LOCUS       DAOMEO010000001        12225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_968_length_12225_cov_2.675349, whole genome shotgun sequence.
ACCESSION   DAOMEO010000001 DAOMEO010000000
VERSION     DAOMEO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_12225_cov_2.675349"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..657
                     /locus_tag="PLH31_00005"
     CDS             91..657
                     /locus_tag="PLH31_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HQR87673.1"
                     /translation="MTRNEFMSRLRRGLAGLPAMTIADIVADHEAHFADALAEGRTEA
                     EAAAALGDPDRLARELRADAGLRRWEETKSPSNAGAAIVALIGLGAIDIMFLLPLLMG
                     VLGAMFGFLVAVVALFVSGGFVFAMGPLAAPPGGPMTAFLAGVGLMAAATFLGALLTV
                     ATVGLFNAVVWYARLHFRLLKPAIDNQG"
     gene            668..1465
                     /locus_tag="PLH31_00010"
     CDS             668..1465
                     /locus_tag="PLH31_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HQR87674.1"
                     /translation="MIRNALIIAGASFVLMLGCFAGVAALAGPVILNEGWTIPFDRVE
                     NGAVRRTHLNVNGAKASPVVSRQLAWTGEALIVDLPIDVTYVQGPVAKVEISGPRDVV
                     DRLRIADGRIHMTDDEGDHRDLYADSLTIDRQGIRIHSNADQTRIVVTAPNVSSFKLD
                     GSGDLEIQAYNQPALTLALNGSGDVNAVGKTTGLVVAAAGSGDAELDDLRALNAEIAI
                     SGSGNADISASNEVKIAISGSGDVNMGLQPTSETSTISGSGSVRHNY"
     gene            1517..>2084
                     /locus_tag="PLH31_00015"
     CDS             1517..>2084
                     /locus_tag="PLH31_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQR87675.1"
                     /translation="MNAPISAPSLLDAAGVDPDQARRILGEALAGADDGELFVERSES
                     EAFVFDDGRLKSASYDSGEGFGLRVVAGETAGYAHAAEISEAAIGRAAASASLAKRGY
                     AGVSAEGPRSTNERLYGEDDPLASPAFSDKVSLLQEIDAFARDRDPRIVQVMASMAGE
                     RRVVEILRADGKLVRDVRPLVRVNVQVTV"
     assembly_gap    2085..2094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2095..2950
                     /locus_tag="PLH31_00020"
     CDS             <2095..2950
                     /locus_tag="PLH31_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HQR87676.1"
                     /translation="GRRESGSAGAGGRAGFSAWISPDKWQGQVDEALRQALVNLDAVP
                     CPAGEMDVVLGSGWNGVLLHEAVGHGLEGDFNRKGISAFSGRIGERVAAKGVTVFDDG
                     SLPGRRGSLTIDDEGTPTERTILIEDGILVGYMHDRMSARLMGMHATGNGRRQSYAHM
                     PMPRMTNTGMLAGRDDPAEMIASMKRGLYCANFGGGQVDITNGKFVFQCTEAYLVEDG
                     KITAPVKGATLIGDGPSALTKVTMIGHDFDFDPGIGVCGKSGQGVPVGVGQPSLKITG
                     LTVGGTAV"
     gene            complement(2957..3166)
                     /locus_tag="PLH31_00025"
     CDS             complement(2957..3166)
                     /locus_tag="PLH31_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87677.1"
                     /translation="MPEPHDLALSKLSAGREKDMDWLVAAIEASIIDVDQMRARLSVM
                     PVERVLGGLEILKQRLTIIESRASA"
     gene            complement(3253..3516)
                     /locus_tag="PLH31_00030"
     CDS             complement(3253..3516)
                     /locus_tag="PLH31_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87678.1"
                     /translation="MMTLAKVEHILRAAGNATGQKAFVLIGSSAIFVWSRIVPATLAM
                     SREADLFATTPDLEEADCIADELDASWARLRHSTMSMDIIATA"
     gene            complement(3513..3860)
                     /locus_tag="PLH31_00035"
     CDS             complement(3513..3860)
                     /locus_tag="PLH31_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87679.1"
                     /translation="MTAGDRFIQSAPLKARFRDAHERRAYQRALEVARRIVDDPSLLE
                     KGRAFLDRFVKDDPRQRRGYALWIETLRLEPEQVVRLLIADDEQGAFLRETAPVFTTI
                     SPDMARQLTSRSA"
     gene            complement(3978..5876)
                     /locus_tag="PLH31_00040"
     CDS             complement(3978..5876)
                     /locus_tag="PLH31_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQR87680.1"
                     /translation="MNIVIALGILVTLVTGIPVLIQLLRQHPKGLIILFFAEMWERFS
                     YYGMRAILIFYLTQHFLFEPKVASAHYGSYTSLVYLLPLIGGFLADKYLGTRKAVAFG
                     AILLVAGHLAMAIEGKPAVQTLTYAGQTYEFQAEGRGQERVAKLIVAGQPYDVSATPT
                     GDFEIKNLPAAAALPAVLPKADYTLAVKDRDPLYLNIFWLALSLIIVGVGFLKPNIST
                     IVGQLYPQGDPRRDAGFNLYYYGINLGSFWASILCGLLGVTVGWWAGFGLAGIGMAAG
                     FVVFVLGKPWLLGKGEPPEPKALKTKVAGPINREIAIYALSLVGVAGVFFLVQFNAIV
                     GLALNIGIVASLGYIGWFMVRKCEKVERERLALAVFLIFGAVVFWTLFEQAGSSLSLF
                     AATNVQLDLVKAPVQLFGGAVTLATSEQLVAAGIDKASTFWVNTSFNAPQTQALNAGW
                     ILIFAPIFAALWTFLGKRGQDLNPVVKFGLALAQVGAGFLILQWGATFADGAFRMPLV
                     FLVLMYMLHTTGEMCLSPVGLSQMTKLSPASVVSFMMAVWFLAVAIAQWVGGKIAGLT
                     ATETVGGQVLNPAAALEASLQVFTVIGLVSIGIGILFLVLSPWLKKWSHGSDDTDAAP
                     VETTATIH"
     gene            6063..6596
                     /locus_tag="PLH31_00045"
     CDS             6063..6596
                     /locus_tag="PLH31_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HQR87681.1"
                     /translation="MKTPDENHPITLEREQARVRVLFEGHEIADSADVIVLREAGYPP
                     VRYFPRDDVRMMFLRRTDKVTHCPYKGAAAYFTIYRDGQVIDNAVWSYEDPYSAVGGI
                     GGRVAFYPQHVEFQVGDLAAEETDAIDVNAVVRHTDSGAGTSQAERWAPTVEDPSSQI
                     VDEAPVVAQPYRETGSI"
     gene            6722..6871
                     /locus_tag="PLH31_00050"
     CDS             6722..6871
                     /locus_tag="PLH31_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87682.1"
                     /translation="MAYISFDQAPSARAFRKAPARRLAWERIGAVAFSLAFWVGLTLA
                     IRAIF"
     gene            complement(6874..7482)
                     /gene="recR"
                     /locus_tag="PLH31_00055"
     CDS             complement(6874..7482)
                     /gene="recR"
                     /locus_tag="PLH31_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQR87683.1"
                     /translation="MPASAGPEIERLIALLSKLPGLGPRSGRRAALALLKKRDVLLAP
                     LTAAMIDAQAKVRTCTVCGSLDTMEPCAVCADETRDGRLICVVEEVGSLWAMERGGQF
                     KGRYHVLGGLLSALDGIGPEALRIGELLARVQGGEVAEVILALPATVDGQTTAHYLAD
                     RLANSNAGVSVTMLARGVPVGGDLDWLDDGTIAQALRARRPA"
     gene            complement(7486..7815)
                     /locus_tag="PLH31_00060"
     CDS             complement(7486..7815)
                     /locus_tag="PLH31_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQR87684.1"
                     /translation="MKDLGGLMKQAQAMQQKLADAQARLAETLVEGTSGGGMVTVTLR
                     GTGELAKVLLDESLIEPGEGEVIADLIVAAHADAKKKLDAAQAQLMQEAAGPMAGMMG
                     GLPGMKF"
     gene            complement(7975..9804)
                     /locus_tag="PLH31_00065"
     CDS             complement(7975..9804)
                     /locus_tag="PLH31_00065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQR87685.1"
                     /translation="MADHDDLTSDSAPPWEEEPAERDENTADIFGAPPAEPVAAPAVV
                     APTPVAVEAPSDPDAAYTVLARKYRPRTFEDLIGQEAMVRTLANAFSSGRIAHAFMLT
                     GVRGVGKTTTARLLARALNYETDTVHQPSVDLTVDGRHCKSIIEGRHMDVLELDAASR
                     TKVDEMRELLDGVRYAPVEARYKVYIIDEVHMLSTAAFNALLKTLEEPPPHAKFIFAT
                     TEIRKVPVTILSRTQRFDLRRVEPDVLVKHFDRIAAKEGARVDADGLALIARAAEGSV
                     RDGLSLLDQAIVQADRGATVSAAVIRDMLGLADRGQTIALFEAVMSGKPADALEGFRA
                     LWGFGADPAVVMLDLLDHCHGSAVAKALGPDALTLPKEQAARLAAIGAHTSAGTLSRL
                     WQMLLKAHEEVRRAPDAMAAAEMAIIRLCYAADLPGPEEALKAMRDGTSVGGGAPGGG
                     SSGGGGGSGGVSAQIVSMASPAAQAMPVLASFDDVLALIAAKRDIGLRLDVEQFVRPI
                     SFRPGAITYEPAPGAPGNLAGRLVRALKEWTGQPWLVAAEGGGGAETLMERQKREDRE
                     AMAQIKADPFVAALLSAFPGSELEVRKIPGPELAPIEPDEVED"
     gene            complement(9891..9983)
                     /gene="ffs"
                     /locus_tag="PLH31_00070"
     ncRNA           complement(9891..9983)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLH31_00070"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            10070..11011
                     /gene="nudC"
                     /locus_tag="PLH31_00075"
     CDS             10070..11011
                     /gene="nudC"
                     /locus_tag="PLH31_00075"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HQR87686.1"
                     /translation="MALSIITNTFAGNPLDRSSERRGDASWLAEKLADAGSLAVAIWN
                     GKPLVEDVLGEDGKPTGAQIAYLRADMAQELAGGPEKLLFLGLWKDIAVFAVDLDSAA
                     DPAEGPLQGLGRFEELRGIAASMPPADAGILATARSMFEWRRRHRWCSACGQPSEVGD
                     GGWKRICPACKAEHFPRTDPVAIMLAVHEDKCLLGRQAMWPEGMFSALAGFLEPGETI
                     EEACARELMEEAGLTATSVRYHSSQPWPWPSSLMMGLIAQVDSNEARPDQTELEEVRW
                     FTKEEAGALIRGELEGVFAPPALAIAHQLIKAWVEEA"
     gene            complement(11145..>12225)
                     /locus_tag="PLH31_00080"
     CDS             complement(11145..>12225)
                     /locus_tag="PLH31_00080"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HQR87687.1"
                     /translation="ASIDLNQADVRSEDIARFFPTTDLAFIGDEIADGVWDAALEETR
                     PLALFDGLRTDFSLARLKHYTGAPAEHVQQFILFTNYHRYVDEFVRWGIEQLQAEGSP
                     YEGMSCSGGLMLTANTVNPELAVAESTWRKHQMPAYHLMGKNGTGITLVNIGVGPSNA
                     KTICDHLAVLRPQAWLMIGHCGGLRDTQTIGDYVLAHAYLRDDHVLDAVLPPEIPVPS
                     IAEVQRALYDASKAISGDSGDQLKKRLRTGTVVTTDDRNWELRHSLSALRFNQSRAVA
                     IDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANAFYERAISQHLQIGILT
                     CKLLLDEGANLHSRKLRAFDEPPFR"
BASE COUNT         2065 a         4095 c         4126 g         1929 t
ORIGIN      
        1 gacgtcggct ggccgcgcca gcttcacgca gcaaaaggcc gaatgggccg ccttcaccac
       61 cgccgtcgaa gatatactgg gagccgccgc atgacccgta atgagttcat gtcccggctg
      121 cgccggggtc tggccggcct gcccgccatg acgatcgccg acatcgtcgc cgatcacgag
      181 gcccacttcg ccgacgccct ggccgaaggc cgcaccgagg ccgaagcggc tgccgccctg
      241 ggcgatcccg accgcctggc ccgcgaactg cgcgccgacg ccggtctccg gcgctgggag
      301 gagaccaaga gcccctccaa cgccggcgcc gccatcgtgg cgctgatcgg cctgggcgcc
      361 atcgacatca tgttcctgct gcccctgctg atgggcgtgc tgggcgcgat gttcggcttc
      421 ctggtcgccg tggtcgccct gttcgtctcg ggcggcttcg tcttcgcgat gggacctttg
      481 gccgcgcctc ccggcggacc gatgacagcc ttcctggccg gcgtgggcct gatggccgcc
      541 gccaccttcc tgggcgcgct gctcaccgtg gccacggtcg gcctgttcaa cgcggtcgtc
      601 tggtacgccc ggctgcactt ccggctgctg aagccggcca tcgacaacca aggctgaggg
      661 gcccgtcatg atccgtaatg cgctcatcat cgccggcgcc agcttcgtgc tgatgcttgg
      721 ctgcttcgcc ggcgtcgccg ccctggccgg tccggtcatc ctgaacgagg gctggaccat
      781 cccctttgat cgtgtcgaaa acggcgcggt ccggcgaacc catctgaacg tcaacggcgc
      841 caaggcctcg ccggtcgtct cccgccagct ggcctggacc ggcgaggcgc tgatcgtcga
      901 cctgccgatc gacgtcacct atgtgcaggg gccggtcgcc aaggtcgaga tcagcggccc
      961 ccgcgacgtc gtcgatcgtc tgaggatcgc cgacggccgc atccacatga ccgatgacga
     1021 gggcgaccat cgcgacctct atgccgacag cctgaccatc gaccgccagg gcatccgcat
     1081 ccacagcaac gccgaccaga cgcgcatcgt ggtcaccgcg ccgaacgtca gcagcttcaa
     1141 gctggatggc tcgggcgatc tggagatcca ggcctacaac cagccagccc tgaccctggc
     1201 gctcaacggc agcggcgatg tcaatgcggt cggcaagacc acgggcctag tcgtcgccgc
     1261 ggccggatcg ggtgacgctg aactggacga cctgcgggcg ctcaacgccg agatcgccat
     1321 ttccggcagc ggcaacgccg acatctcggc cagcaacgag gtcaagatcg ccatctcggg
     1381 cagcggcgac gtcaatatgg gcctgcagcc gaccagcgag accagcacga ttagcggctc
     1441 gggttcggtg cgtcacaact actaacgaca agggggcggg gtgacggaac ggtttcgaac
     1501 gcttacctgt aggcgcatga acgctccgat ctccgcccct tccctgctcg acgccgccgg
     1561 tgtcgatccc gatcaagccc gcagaatcct gggcgaggcc ctggccggcg ccgacgacgg
     1621 cgaactcttc gtcgaacggt cggaaagcga agccttcgtg ttcgacgacg gccgtctcaa
     1681 gagcgcctcc tatgattcgg gcgagggctt tggccttcgc gtcgtggccg gcgagaccgc
     1741 cggttacgca cacgccgccg agatttccga ggccgccatc ggccgggctg ccgcctcggc
     1801 tagcctcgcc aagcgcggct atgcgggcgt cagcgccgaa gggccccgtt cgaccaacga
     1861 gcggctgtac ggcgaggatg accccctcgc ctcgcccgcc ttctccgaca aggtttccct
     1921 gcttcaggaa atcgacgcct tcgcccgcga ccgcgacccg cgcatcgtcc aggtgatggc
     1981 ctcgatggcc ggcgaacgcc gcgtggtcga gattctgcgc gctgacggca agctggtgcg
     2041 cgatgtccgc ccgctggtgc gcgtcaatgt gcaggtcacc gtcgnnnnnn nnnncggccg
     2101 ccgcgagagc ggctcggccg gcgccggcgg ccgcgcaggc ttctcggcct ggatcagccc
     2161 ggacaaatgg cagggccaag tggacgaggc cctgcgccag gcgctggtca atctggacgc
     2221 cgtcccctgc cccgccggcg agatggacgt ggtgctgggc tcgggctgga acggcgtgct
     2281 gctgcacgag gccgtcggcc acggtctgga aggcgacttc aaccgcaagg gcatttcggc
     2341 cttctccggc cgcatcggcg aacgcgtggc ggccaagggc gttaccgtgt tcgacgacgg
     2401 ctcgctgcca ggccgccgcg gctctctgac catcgacgac gagggcacgc cgaccgagcg
     2461 caccatcctg atcgaggatg gcatcctggt gggctacatg cacgaccgca tgagcgcccg
     2521 cctgatgggc atgcacgcca ccggcaacgg tcgccgccag tcctatgccc acatgcccat
     2581 gccgcgcatg accaataccg gcatgctggc cggcagggac gatccggccg agatgatcgc
     2641 ctcgatgaag cgcggcctct attgcgccaa tttcggcggc ggccaggtgg acatcaccaa
     2701 cggcaagttc gtcttccagt gtaccgaggc ctacctcgtc gaggacggca agatcaccgc
     2761 cccggtcaag ggcgccaccc tgatcggcga cggccccagc gccctgacca aggtgacgat
     2821 gatcggccac gatttcgatt tcgaccccgg tatcggcgtc tgcggcaagt ctggccaggg
     2881 cgtgccagtc ggcgtgggcc agccgtcgct gaagatcacc ggcctgacgg tgggcgggac
     2941 ggcggtttaa ggcggatcag gccgacgcgc ggctttcgat gatcgtcagc cgttgcttca
     3001 ggatttcgag gccgcccaag acgcgctcga caggcatgac tgacagccgc gcccgcattt
     3061 ggtcgacatc gatgatggac gcctcaatgg ccgccaccag ccagtccata tccttctcac
     3121 ggccggcgct gagtttcgag agcgccagat cgtggggctc cggcacgagc gcggtgacgc
     3181 cgcccgtatt ggcgctagaa tagagcttgg cgcggtctcg ccaatcggca ggcagtatgg
     3241 ccgtctcagg ccctacgccg tcgcaataat atccatactc atcgtcgaat ggcgaagcct
     3301 ggcccaggag gcgtccagtt cgtcggcgat gcaatcggct tcttcgaggt caggcgtggt
     3361 ggcgaacagg tcggcctcgc gcgacatcgc cagcgtcgcg ggcacgatcc gcgaccagac
     3421 gaaaattgcc gacgacccga tcaagacgaa agccttttga cccgtggcat tgccggcggc
     3481 gcgcaggatg tgctccacct tggcaagcgt catcatgccg agcggctcgt cagttggcgc
     3541 gccatatccg gagaaatcgt cgtgaagacg ggcgcggttt cccgcagaaa agcgccttgt
     3601 tcgtcatcgg cgatcagaag gcgaacaacc tgttccggct ccaggcgcag ggtctcgatc
     3661 cataaggcgt acccgcgtcg ctgacgtgga tcgtccttca cgaagcgatc caggaacgcc
     3721 cgcccctttt ccagcagaga cgggtcgtcg acgatccgcc gggccacttc cagcgcacgt
     3781 tgataggcgc gacgctcgtg agcatcgcgg aaacgcgcct tcaatggagc ggactggatg
     3841 aagcggtcgc cggctgtcat ttcatccaca gtctacacga agtcgcgaag aaggggattg
     3901 atgtcccgct cgccgcccac ccaacaaaaa aggccccgga tcgctccggg gccttcgttg
     3961 ttttaagcgt cccgcgatca gtggatcgtg gcggtcgttt cgaccggcgc agcgtcagta
     4021 tcgtccgagc cgtgcgacca cttcttcagc cagggcgaga gaaccaggaa caggatcccg
     4081 atcccgatcg agaccaggcc gatgacggtg aagacctgca gcgaggcctc cagcgccgcg
     4141 gcggggttca ggacctggcc gccgacggtt tcggtcgccg tcagaccggc gatcttgccg
     4201 cccacccact gagcgatggc cacggccagg aaccagacgg ccatcatgaa cgacaccacc
     4261 gaggcgggcg acagcttggt catctgcgac aggcccacag gggacaggca catctcgccg
     4321 gtcgtgtgca gcatgtacat cagcaccagg aagaccagcg gcatccggaa ggcgccgtcg
     4381 gcgaaggtgg cgccccactg caggatcagg aagccggcgc cgacctgggc cagggccagg
     4441 ccgaacttga cgaccgggtt gagatcctgg ccgcgcttgc ccaggaaggt ccacagcgcc
     4501 gcgaagatcg gggcgaagat caggatccag ccggcgttca gggcctgggt ctggggcgcg
     4561 ttgaagctgg tattgaccca gaaggtcgag gccttgtcga tgccggccgc caccagttgc
     4621 tcggacgtgg ccagggtgac cgcgccgccg aacaactgga ccggagcctt gaccagatcg
     4681 agctggacat tggtggccgc aaacaggctg agcgatgagc cggcctgttc gaacagggtc
     4741 cagaacacca ccgcgccgaa gatcaggaac acggccaggg ccaggcgctc gcgctcgacc
     4801 ttttcgcact tcctgaccat gaaccagccg atatagccca gcgaggccac gatgccgatg
     4861 ttcagggcca ggccgacgat ggcgttgaac tggaccagga agaacacgcc cgccacgccg
     4921 accagcgaca gggcgtagat ggcgatttcg cgattgatcg ggccggcgac cttggtcttc
     4981 agggccttgg gctcgggcgg ctcgcccttg cccagcagcc agggcttgcc cagcacgaac
     5041 acgacaaacc cggccgccat gccgatgccg gccaggccaa agcccgccca ccagccgacg
     5101 gtgacgccca gcaggccgca caggatcgag gcccagaacg agcccaggtt gatgccgtaa
     5161 tagtagaggt tgaagccggc gtcgcgacgc gggtcgccct gcggatacag ctggccgacg
     5221 atggtcgaga tgttgggctt gaggaagccg acaccgacga tgatcagcga cagggccagc
     5281 cagaagatgt tgagatacag cggatcgcga tccttcacgg ccagggtgta gtcggccttg
     5341 ggcaggacgg cgggcagggc ggcggcggcg ggcaggttct tgatctcgaa gtcacccgtc
     5401 ggggtggccg agacatcata gggctggccg gcgacgatca gcttggcgac gcgctcctgg
     5461 ccccggccct cggcctggaa ctcataggtc tggccggcat aggtcagggt ctgaacggcg
     5521 ggcttgccct cgatcgccat ggccaggtgg cccgcgacca gcagaatcgc cccgaacgcc
     5581 accgccttgc gggtgcccag atacttgtcg gccaggaagc cgccgatcaa cggcagcaga
     5641 tagaccagcg aggtatagga tccgtaatga gccgaggcga ccttgggctc gaaaaggaaa
     5701 tgctgggtca gatagaagat caggatggct ctcatgccgt agtaggagaa tcgctcccac
     5761 atctcggcga agaacaggat gatcagcccc ttgggatgtt gtcggagcag ctggataagc
     5821 acgggaatgc cggtcaccag cgtgaccagg atgcccaggg cgataacgat gttcatacac
     5881 taaccttgac gtgatgaggg gtcgggacgg ccgccatggt cgtttcgccc tcgcctcccc
     5941 cagttggtgg ggagaagatc acgcgacaat gcgtttaaca agcaatggct tgccagctgg
     6001 gagccgagtt cgcgtcctgc gcgtttgacg gacgcgccca ttccacgtgc gggagaaaat
     6061 cgatgaagac gccggacgag aaccatccga tcacgctcga acgcgaacag gcccgggtgc
     6121 gggtgctgtt cgagggtcac gagatcgccg acagcgccga cgtgatcgtg ctgcgcgagg
     6181 cgggctatcc gccggtgcgc tacttcccgc gcgacgatgt gcggatgatg ttcctgcgtc
     6241 gcaccgacaa ggtgacccac tgcccctaca agggcgcggc ggcctatttc acgatctatc
     6301 gcgacggtca ggtgatcgac aatgcggtgt ggtcctatga ggacccctac tccgcagtcg
     6361 gcggcatcgg cggtcgggtg gccttctatc cgcagcatgt cgagtttcag gtcggtgacc
     6421 tagccgccga agagaccgat gcgatcgatg tcaacgccgt ggtgcgacac actgacagcg
     6481 gggcgggaac cagccaggcc gagcgctggg cgccgacggt cgaggacccg agcagccaga
     6541 tcgtcgacga agcgcccgtc gtggcccagc cctaccgcga gaccggctcg atctagccct
     6601 gcggtggggc gctttgacgc gccccacccg ggttaaaggt aaggagtcgt taagggcgcc
     6661 gggcgttgca tgggactcac gcaaacgcgt gcagcctcca cagaggggca aggaggaagc
     6721 catggcctat atcagctttg atcaagcgcc gtccgcccgc gcctttcgca aggcccccgc
     6781 gcgccgtctg gcctgggagc gcatcggcgc ggtggcgttc agcctggcct tctgggtcgg
     6841 cctgaccctg gcgatccgcg cgatcttcta gcttcaggcc ggccgccggg ctcgcagggc
     6901 ctgggcaatc gtgccgtcat ccagccagtc caggtcgccg ccgaccggaa ccccgcgcgc
     6961 cagcatcgtc accgacacgc ccgcgtttga gttcgccagc cggtcggcta gatagtgcgc
     7021 cgtcgtctgg ccatccaccg tagccggcag ggccaggatg acctcggcca cctcgccgcc
     7081 ctgcacccgc gccagcagtt cgccaatacg cagggcctcg ggcccgatcc cgtccagcgc
     7141 tgacaaaagc ccgcccagca cgtgatagcg gcccttgaac tgcccgccgc gctccatggc
     7201 ccatagcgag cccacctctt cgaccacgca gatcagccgg ccgtcccgcg tctcgtcggc
     7261 gcagacagcg cagggctcca tcgtatccag cgagccgcat accgtgcagg tccgcacctt
     7321 ggcctgggcg tcgatcatcg ccgccgttag cggggccagc agcacgtcgc gcttcttgag
     7381 aagcgccaga gccgcccgcc ggcccgaacg cggtcccaac cccggcagct tggacagcag
     7441 ggcgatcagg cgctcgatct cgggtccggc ggaggcgggc aaggcctaga acttcatccc
     7501 aggcaggccg cccatcatgc cggccatggg accggcggct tcctgcatca gctgggcctg
     7561 ggcggcgtcc agcttcttct tggcgtcggc gtgggcggcg acgatcaggt cggcaatgac
     7621 ctcgccctca cccggctcga tcaggctttc gtccagcaac accttggcca gctcgccggt
     7681 gccccggagg gtgacggtga ccatgccacc gcccgaggtt ccctcgacca gggtctcggc
     7741 cagccgcgcc tgggcgtcgg ccagtttctg ctgcatcgcc tgagcctgct tcatcaggcc
     7801 gccgaggtct ttcatggtct gctccatctc ataaacacgt tccctccccc ttggatgggg
     7861 gaggggcagg ggtgggggtg acaccgccgg acgatttcac atcgaccgag ccgcaccctg
     7921 aaacgctttc acccccatcc ccaacccttc ccccatctag ggggaaggga gaaatcagtc
     7981 ctcaacctcg tccggctcga tcggcgccag ctctggtccc gggatcttgc gaacttccag
     8041 ctcggagccc ggaaaggcgc tgagcagcgc ggcgacgaac ggatcggcct tgatctgcgc
     8101 catcgcctcg cggtcttcgc gcttctggcg ctccatcagg gtctcggccc cgccgccgcc
     8161 ctcggcggcg accagccagg gctggccggt ccattccttc aacgcgcgaa ccagacgacc
     8221 ggccagattg ccgggcgcgc caggggcggg ctcataggtg atggcgcccg gccggaagct
     8281 gatcgggcga acgaactgct cgacatccag ccgcaggccg atgtcgcgct tggcggcgat
     8341 cagggccagc acatcgtcga acgaggccag cacaggcatg gcctgggccg ccggcgaggc
     8401 cattgaaacg atctgggcgc tgacgccgcc ggagccgccg ccgccgcccg atgaaccgcc
     8461 gccgggcgcg ccgccgccga cggaggtccc gtcgcgcatc gccttgaggg cttcttcggg
     8521 acctggcaga tcggccgcat agcacaggcg gatgatggcc atctcggcgg cggccatggc
     8581 gtcgggcgcg cggcgcacct cctcatgggc cttcagcaac atctgccaca ggcgcgacag
     8641 ggtgccggcc gaggtatggg ccccgatggc ggccaggcgg gcggcctgtt ccttgggcag
     8701 ggtcagggcg tcgggcccca aggccttggc cacggccgaa ccgtggcaat ggtccagcag
     8761 atccagcatc accaccgccg gatcggcccc gaaaccccac agggcgcgga aaccttccag
     8821 ggcgtcggcg ggcttgccgc tcatcaccgc ttcgaacagg gcgatggtct ggccgcgatc
     8881 ggccaggccc agcatgtcgc ggatcacggc ggccgagacg gtggccccgc ggtcggcctg
     8941 gacgatggcc tggtcgagaa gggacaggcc atcacgcacc gaaccctcgg cggcccgggc
     9001 gatcagggcc aggccgtcgg cgtcgacccg cgcgccctcc ttggcggcga tcctgtcgaa
     9061 gtgcttgacc agcacgtccg gctcgacccg gcgcaggtca aaacgctggg tgcgcgacag
     9121 gatggtcaca ggaaccttgc ggatctcggt ggtggcgaag atgaacttgg cgtgcggcgg
     9181 cggctcttcc agtgtcttca gcagcgcgtt gaacgccgcc gtcgacagca tgtgcacttc
     9241 gtcgatgatg tagactttat agcgcgcctc gaccggggcg tagcgcaccc cgtccagcag
     9301 ctcgcgcatc tcgtcgacct tggtgcgcga ggcggcgtcc agttccagca cgtccatgtg
     9361 ccggccttcg atgatcgact tgcagtgccg gccatcgacc gtgaggtcga cagagggctg
     9421 atggacggtg tcggtctcgt agttcagggc gcgggccagc aggcgggcgg tcgtagtctt
     9481 gcccaccccc cgcacgccgg tcagcatgaa ggcgtgggcg atgcggccgc tggaaaacgc
     9541 gttggccagg gtgcggacca tggcctcctg gccgatcagg tcctcgaagg tgcgcggacg
     9601 atatttgcgc gccaggacgg tgtaggcggc gtccggatcg ctcggcgctt cgacggcgac
     9661 cggggtcggc gcgacgacgg cgggcgcggc cacgggctca gccggcggcg cgccgaagat
     9721 gtcggcggtg ttctcgtccc gctcggcggg ctcttcttcc cacggcggcg cggaatcgga
     9781 tgtgaggtcg tcgtggtcgg ccatggcgac aactgtagag cgccggagcg cccagcaaaa
     9841 ggctcgctcg cgtccggcga ccatctgctg acagatttaa gcgggaaagg tggagaccgg
     9901 cggacgaccc gaagcttggc tcgttgtggc tgctgcctct cggccctgac cagattggcg
     9961 agggctccgc ccgtcaccga tctccgaggc ctatatcgcg agtcatcgtc ccatatgcaa
    10021 gaccgcgcga cgtcgtgacg actcttcgtg gctgggctac aggaatcgca tggccctctc
    10081 gatcattacg aataccttcg ccggcaaccc tctggaccgc tccagcgagc gacggggcga
    10141 cgcgtcctgg ctggctgaaa agctggccga cgccgggtcg ctggccgtgg cgatctggaa
    10201 tggcaagcct ctggtcgagg atgtgctggg cgaagacggc aagccaaccg gcgcccagat
    10261 cgcctatctg cgcgccgaca tggcccagga gctggcgggc gggcccgaga agctgctgtt
    10321 tctgggactg tggaaggaca tcgcggtctt tgcggtggat ctggatagtg cggccgatcc
    10381 agccgagggg ccgctgcagg gtctgggccg gttcgaggaa ctgcgcggga tcgccgcctc
    10441 gatgccgccg gccgatgcgg ggattctggc caccgcccgc tcgatgtttg aatggcgtcg
    10501 ccgccatcgc tggtgcagcg cttgcggcca gccctccgaa gtcggcgacg gcggctggaa
    10561 acgcatctgc cccgcctgca aggccgagca ttttccacgc accgacccgg tagcgatcat
    10621 gctggccgtc catgaggaca agtgcctgct gggtcgtcag gcgatgtggc ccgagggcat
    10681 gttctcggcc ctggccggat tcctcgagcc cggcgagacc atcgaggaag cctgcgcccg
    10741 cgagctgatg gaagaggccg gcctgacggc gaccagcgtc cgctatcact ccagccagcc
    10801 gtggccatgg ccatcatcgc tgatgatggg cctgatcgcc caggtcgaca gcaacgaggc
    10861 tcgccccgac cagaccgagc tggaagaagt gcgctggttc accaaggaag aggccggggc
    10921 gctgatccgg ggggaactgg agggggtgtt cgccccgccg gccttggcaa ttgcgcatca
    10981 gctgatcaag gcgtgggtgg aagaggctta ggcggtagct gctcccccca cgggggagct
    11041 gtcgcggagc gactgagggg gccatcgcgg cagtcgccca gctagccccc tccgaccctc
    11101 cgggccacct cccccagagg gggaggagag gagctatttc gcgatcaccg gaacggcggc
    11161 tcgtcaaacg cccgcagctt gcgcgagtgc aggttggcgc cctcgtcaag cagcagtttg
    11221 caggtgagaa tcccgatctg caggtgctgg ctgatcgccc gctcgtagaa ggcgttggcc
    11281 tggccgggca gcttgatctc gccgtgcagc ggcttgtccg acacgcacag cagggtcccg
    11341 tagggcacgc ggaagcgata gccctgggcg gcgatggtgg ctgactccat gtcgatggcc
    11401 accgcgcggc tctggttgaa gcgcagggcc gacaggctgt ggcgaagctc ccagttgcgg
    11461 tcgtcggtgg tgaccacggt gccggtgcgc agacgcttct tcagctggtc gccgctgtcg
    11521 ccgctgatgg ctttcgaggc gtcatagagg gcgcgctgca cctcggcgat cgacggcacc
    11581 gggatctccg gcggcagcac ggcgtccagc acgtggtcgt cgcgcagata ggcgtgggcc
    11641 agcacatagt cgccgatggt ctgggtgtcg cgcaggccgc cgcagtggcc gatcatcagc
    11701 caggcctggg gccgcagcac ggccaggtgg tcgcagatgg tcttggcgtt ggagggaccg
    11761 acgccgatat tcaccagcgt gatccccgtg ccgttcttgc ccatcaggtg ataggccggc
    11821 atctggtgct tgcgccaggt cgactcggcc accgccagtt ccggattgac cgtattggcc
    11881 gtcagcatca gaccgccgga gcacgacatg ccctcatagg gactgccctc cgcctgcaac
    11941 tgctcgatcc cccagcggac gaattcgtcg acatagcgat gatagttggt gaacaggatg
    12001 aactgctgca cgtgctcggc gggggcgccg gtatagtgct tcaggcgggc cagcgagaag
    12061 tcggtgcgca ggccgtcgaa cagggccagc ggccgagtct cttccagcgc cgcatcccag
    12121 acgccgtcag cgatctcgtc gccgatgaag gccaggtccg tggtcgggaa gaagcgcgcg
    12181 atgtcctcgc tgcgcacatc ggcctggttc aggtcgatgc tggcg
//
LOCUS       DAOMEO010000002        10746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1092_length_10746_cov_2.728089, whole genome shotgun sequence.
ACCESSION   DAOMEO010000002 DAOMEO010000000
VERSION     DAOMEO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_10746_cov_2.728089"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLH31_00085"
     CDS             <1..481
                     /locus_tag="PLH31_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HQR87688.1"
                     /translation="AEDKMPVGYWTAMEEDAHGLKVEGQLDPIDTERGRTLLAGLRNG
                     SIDAMSITYSAVDFAYGKATNDPYRTITKVELYEVGPVLWGMNPLAGIDDAKAASRIR
                     TIRDFEAFLRDAGGFSHAAAKAIANGGYKANPKPRDEGGMDDLLDLQRQAERLFSTT"
     gene            495..1862
                     /locus_tag="PLH31_00090"
     CDS             495..1862
                     /locus_tag="PLH31_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HQR87689.1"
                     /translation="MHEHNRAVGRAHALERKDASDLPAVLAEVKKIVTPLMTGFEEYK
                     KTNDQRLREIETTGKADPLTEEKLARIEATLSGFEGLNQKLVNAEALEAKRAEREKQM
                     QEQLDRLQLKVQRPGMGSEEARIERKAYFNDWARGVIGAYTTGVPNLPEPQAKAIARA
                     TTEFKSLSVSNDTTGGYLAPAEYVADIIKGITLVSPVRSLVSVKPTANKSRMQPRRTG
                     QFAAQWVAEQGIRSETTGLAYGMVEIPTHEMFALIDISHQNLEDTAFDLEAEIRGEAE
                     EQFAVAEGAAVVSGNAVGKPEGWLVNADIASTNSGTAATIADANGQADGLLTLKYALK
                     SGYARNAKWALNRTTMGSVRKLKDANRQYIWMPGLAQGQPNTIDGDPYVEVPDMPNEG
                     ANAFPIVYGDFARAYTLVDRIAMSMLRDPYTQATSGNIRFLFYRRIGGQIVLAEAIRK
                     LKCST"
     gene            1936..2364
                     /locus_tag="PLH31_00095"
     CDS             1936..2364
                     /locus_tag="PLH31_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87690.1"
                     /translation="MARDIHNSLHVRRGISPAAAVVDNTPFVSQIADLLGYEAAEFVI
                     LTGALADADATFTTLVEHGDAANMSDAAAVPDDQLIGLETQASFTFADDDKVFKIGYR
                     GPKRYARVTVTPAANTGNAFVAGVWVLGHPRNRPTANPPA"
     gene            2552..3214
                     /locus_tag="PLH31_00100"
     CDS             2552..3214
                     /locus_tag="PLH31_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87691.1"
                     /translation="MSEQLFEVVTPVSNAAARRLTTAEKVQAALRLGSVDSTLIESII
                     DAVSGECVRFCNLARPATGGVPTFGQEALRATWLVTALYRGSVLILPWRTPVATVDSV
                     VEDGTTLALNTDFRLVCGGMLERMTDDAPVSWSPGKIVVSWTAGWSLPTEVPAELEGQ
                     VIEQVKMKYLATDRDPALRSENTPDVWSGSYAVAGGDSIGESGLLKSLEAALSPFKTW
                     AV"
     gene            3202..3546
                     /locus_tag="PLH31_00105"
     CDS             3202..3546
                     /locus_tag="PLH31_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87692.1"
                     /translation="MGGVMAALDVVRNAARLIALHGETMVLKRASEATTITLKGKRLV
                     GSTVDIGGSAVQQEFRVKIGPSELSSSAWTSKAPVRHDSIVIGDRERSILDVRPLGDG
                     GTVALYELLVAG"
     gene            3548..4219
                     /locus_tag="PLH31_00110"
     CDS             3548..4219
                     /locus_tag="PLH31_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87693.1"
                     /translation="MPVIVEGITTEQLGRSIADWVKAATIQTAERALREEVARGFDTE
                     PVVITDGMPRRDYLQVKPFGRIEFAARTSMVEAVRWALTELQNKSPVLTGRYASSHTV
                     MINGAEVEGNIWVALRNARPTDRVQIVNPQPYARKIEGATANRRTGRGKRAALSRQAR
                     SGVYRVVLRALVNRFGKALFFDFKYVNLNIGIKVWGKQGARRVQRDQVYPALQFFIKP
                     TGLPN"
     gene            4229..4726
                     /locus_tag="PLH31_00115"
     CDS             4229..4726
                     /locus_tag="PLH31_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87694.1"
                     /translation="MMYGVHPIASVPLGATATGLVVGDPLRMAFRDRLIVWLADLGIG
                     WPIRDLYNTGENPDVSQGFVALDFPGGTEDQYTFGAPGLNFWREQGQVTLYVKTRLGA
                     GMTMRNLAESYAGALRSRFRNDRFAAGNGAVRITSTGPMGGGHDEAGLWVEAVALGYE
                     TFNIG"
     gene            4809..5738
                     /locus_tag="PLH31_00120"
     CDS             4809..5738
                     /locus_tag="PLH31_00120"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HQR87695.1"
                     /translation="MDSANKQTAVIAEATVGTTPATPAFLLTRDIRVSGAPQRPNTRS
                     PERRSDRQAASMVQGVATYPKQIEMPWVRDAASDVLWASLFCSPWVSNVLKVGSVRSG
                     TTFTLEEKYEGGATDPYRRLTGCMVDNVSIAFQNGNPGQITFSLLGLGETTATTAIAS
                     STYAAPTPAYDPSTPADIVVNSLFGLASPKVRSLQLQISNNLQQQYAFGSADPFAIGL
                     GEFNVQGVVEVYFNALADYSAFVVKQTGQTVDITIGATMNFKDRLVLGNCDVFNPNVD
                     DPGQTGVHAVTLNFLGKYYATDASAMKLTRNAP"
     gene            5759..5920
                     /locus_tag="PLH31_00125"
     CDS             5759..5920
                     /locus_tag="PLH31_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87696.1"
                     /translation="METSVLILQTFHRFVSEGEGRPERKERFAQGQTVKVSEEDAADW
                     IAKGLAREV"
     gene            5972..6400
                     /locus_tag="PLH31_00130"
     CDS             5972..6400
                     /locus_tag="PLH31_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87697.1"
                     /translation="MSQFKFGNIDKLKRNREIEGESGTEIGLPGGISLIALCASDANP
                     AWRRGGEDFLAELKRLSRAHASDERVKRFLAEQLARMLVKDWSGVVDQDGNAIPFSID
                     ACMEFLIEADDAIPALQAVVYETQNFRGQRIEAIVDHAKN"
     gene            6562..6786
                     /locus_tag="PLH31_00135"
     CDS             6562..6786
                     /locus_tag="PLH31_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87698.1"
                     /translation="MLSLSLGMGGGLVLPQPIPREAIRRYGRALRLSGEELADFLEIV
                     SGIDDFWVVTEQRKQTDAAVRAARGMSRQR"
     gene            6790..10500
                     /locus_tag="PLH31_00140"
     CDS             6790..10500
                     /locus_tag="PLH31_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87699.1"
                     /translation="MAEETKIIRLVIDSSKAVDGGRAAQRALEQIERGTSSLDSTMAR
                     MEQSLGRVGGMIKAHLALMLAEMAARLVETAKAAVEAVGGLDELAEQLGLTTKGLQAL
                     QFSGIQNGLKLEQLETGAAKFSQKIGEAANGSKEMIEALDHLGVRILDVDKTLRPTED
                     IMSDVARAILAIEDPARRAAASVEFFGKAGTRFQPVLQDIARGFGFMGEEARKGGALV
                     DEHVVKRLDQLSDAFERGKLRSRAFFAEMAVAVLDMADKAVDAYRKILAQQATRSSGG
                     GYQWERDFQSWVENAVSNMAGVGAGVVEVFRGAAEQVVKLWIDAFNRVLAGAEQVLNK
                     LKEFSTFGLGDAIALGRIPGGGASVNDFVGGITQAAETARADTQAYYARQRSPFGKAS
                     SGVAWAVVNDGLPAQDGPRPGIFNPPVKPGGGGSEDSIDKLKRQTELAMQAAQALADV
                     SAQGSRAVADLELRLKAVKEVQDAYASSAHKAGLSVEELTKRIEDQQRATERAKNLKD
                     FNIETSSLQKANELLEAENRLIDQSTETRAVVIAQIRLKQELELKGIGVATEAERAAI
                     AARGEAIEQNERLKGQAEELKRANELWTEPLKQAFRNIQTAGADAWDRILQSGQVSFQ
                     ALGDIFKTTIRRMVAEFLALATIRPVMSVVVQGLGSLGVVSPAVAQQMGFGGATSGSG
                     SLGGGSIPLPSLGSTGGGSFFSGLGLGGIGDWLNTPFTGPYAGISPSAMQGVPTLSPS
                     MWNPSTWSITPLQGIGAAAGIGMGVYQLANSRSTGQTIAGIGSMVGGAVSLIPGLQPV
                     GMAISLLSQFAPAIFGESNTRTHSSTNASLHYGNGNWYTTGGAYGPNANSGQSESALR
                     GTTAGIDAVLGILGGVKDPSKVWGLNASSWTAQGKDWSYTSNATHLVDPETGSQEAWR
                     MNMDDMMDTGAAQVAIRSILSGAVGEISATMKTALESMRAASMGIKETAESIVFVDDV
                     YERLGKGALAVRTQFRELEKQFGDMTDKATRLGLALAPIEAEQKKATERLGQDYVDNL
                     IDPVAAGLRAWEDEKQSILANIDYIHQHTDVIVDQARIAEALLRKEAALKEQLYGGAI
                     LQLEDAIRRLSLGDLANLSPTTMLTGVRAAYQATVAQARSGDSSAIARVAAEGTTFAQ
                     AAQSYYASGPDYEALKQQILNDLLTLQSQIAGSGGTTGSAMNDNNGATQAAVAQATQL
                     QAVVSEQSRQIAALMAKLSETNDLLLRRAVNG"
     gene            10513..>10746
                     /locus_tag="PLH31_00145"
     CDS             10513..>10746
                     /locus_tag="PLH31_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87700.1"
                     /translation="MASTIYLTGLPGPSGTVDYPALTQFPVDVGADAVDLQSARGLSY
                     LLRAAPPDPGGNIVLSGGAPYPSGMTDWPTPFQV"
BASE COUNT         1964 a         3500 c         3610 g         1672 t
ORIGIN      
        1 ggccgaggac aagatgccgg tcggctactg gactgccatg gaagaggatg cccacggcct
       61 gaaggtcgag ggccagctcg acccgatcga cacggagcgg gggagaacgc tgctcgcggg
      121 cctgcgcaac ggctcgatcg acgccatgtc catcacctat tcggcggtgg acttcgccta
      181 cggcaaggcg accaacgacc cgtaccggac catcaccaag gtcgagctct acgaggtggg
      241 acctgttctc tggggcatga accccctcgc cggcattgac gacgccaagg cggcgtcccg
      301 gatccgcacc attcgcgact tcgaggcctt cctgcgggat gcaggcgggt tctcacatgc
      361 cgccgcaaag gcgatcgcca acggcggcta caaggccaat ccgaaacctc gggacgaggg
      421 cgggatggac gacctcctgg acctgcagcg gcaggccgag aggcttttca gcaccacctg
      481 attccgagga caacatgcac gaacacaatc gcgccgtcgg gcgcgcgcac gcgctcgagc
      541 gcaaggacgc cagcgacctc ccggccgtgc tggccgaggt caagaagatc gtgacgccgc
      601 tgatgacggg cttcgaggag tacaagaaga ccaacgacca gcggctccgc gagatcgaga
      661 ccacgggcaa ggccgatccc ctgaccgagg agaagctcgc ccggatcgag gcgacgctgt
      721 cgggcttcga gggcctgaac cagaagctgg tcaacgccga agcgctggag gccaagcggg
      781 ccgagcgcga gaagcagatg caggagcagc tcgaccggct ccagctgaag gtccagcgac
      841 ccggcatggg ctccgaggaa gcccgcatcg agcgcaaggc ctacttcaac gactgggctc
      901 gtggcgtcat tggcgcctac acgaccggcg tgccgaacct gccggagccc caggccaagg
      961 cgatcgcccg ggcaacgacc gagttcaagt cgctgtcggt cagcaacgac acgaccggcg
     1021 gctacctggc gccggcggag tatgttgccg acatcatcaa gggcatcacc ctggtgagcc
     1081 cggttcgctc gctggtctcg gtcaagccga cggcgaacaa gtcgcggatg cagcccaggc
     1141 gtaccggcca gttcgccgcc cagtgggtag cggagcaggg gatccggtcc gagaccacgg
     1201 gcctggccta cggcatggtc gagatcccga cgcacgagat gttcgccctg atcgacatca
     1261 gccaccagaa cctggaggac acggccttcg atctggaggc cgagatccgc ggcgaagccg
     1321 aggagcagtt cgccgtggcg gaaggggcgg ccgtggtgag cggcaatgcc gtgggcaagc
     1381 ccgagggctg gctggtcaat gccgacatcg cgtccaccaa ctctggcacg gcagccacga
     1441 tcgcggacgc gaacggccag gccgacgggc tgctgaccct caaatacgcg ctgaagagcg
     1501 gctatgcgcg caacgccaag tgggccctga accggaccac gatgggctcg gtgcgaaagc
     1561 tcaaggacgc caacaggcag tacatctgga tgcccggcct ggcgcagggg cagcccaata
     1621 cgatcgacgg cgacccatac gtcgaggtgc cggacatgcc gaacgagggg gccaacgcct
     1681 tcccgatcgt ctacggcgac ttcgcccgcg cctacacgct ggtggatcgc atcgccatgt
     1741 cgatgctgcg ggacccctac acccaggcga ccagtggcaa catccgcttt ctgttctacc
     1801 gccgcatcgg cggccagatc gtgctggccg aggcgatccg caagctgaag tgctcgacct
     1861 gatcggcgac ggcggcgcgg ctccggtcgc gccgctccct tcctcacctt cacgattccc
     1921 ggaaaggaaa gcgccatggc gcgcgatatt cacaatagcc tgcacgtccg tcgcggcatc
     1981 agccccgcgg cggcagtcgt cgacaatacg cccttcgtct cgcagatcgc cgatcttctc
     2041 ggctacgagg ctgccgagtt cgtcatcctg accggcgcgc tcgccgatgc ggacgccacc
     2101 ttcaccaccc tggtggagca tggcgacgcg gccaacatgt cggacgcagc cgccgttcct
     2161 gacgaccagc tgatcggcct cgaaacccaa gcgagcttta ccttcgcgga cgacgacaag
     2221 gtattcaaga tcggctaccg cgggccgaag cgctacgcgc gcgtgacggt cacgccggcg
     2281 gccaataccg gcaacgcctt cgtcgccggc gtgtgggtcc tcggtcatcc gcgcaatcgc
     2341 ccgaccgcca atccgccggc ctgatcatga aggccagggt catcaaggca ttcgtggggg
     2401 cgcccgatgg cgccctccat ccgcggcagt tcgaggtgtc ggagctggtc gagggagatc
     2461 tcgcgagagt cgctattgcc gaggggtggg cagaggcgct gggcgctgcg ccgacggagc
     2521 aagaccctcc gcggcggcga tcgccgagcc ggtgagcgaa cagctgttcg aggtcgtcac
     2581 ccccgtgtcg aacgccgctg cgcggcggct gacgacggcg gagaaggtcc aggcggctct
     2641 ccggctgggc agtgtcgata gcacgttgat cgagagcatc atcgatgccg tcagcgggga
     2701 gtgcgtccga ttctgcaatt tggctcggcc agcgaccggc ggcgtgccga ctttcgggca
     2761 ggaagctctc cgcgccacat ggcttgtgac cgctctgtac cgtggttccg tcctgatcct
     2821 gccgtggcgg acacccgtcg cgacggtgga cagcgtcgtc gaggatggga ccaccctcgc
     2881 cttgaacacc gacttccgcc tcgtttgcgg cggcatgctg gagcgaatga ccgatgacgc
     2941 cccggtctcc tggtcgcccg gcaagatcgt ggtgtcctgg acggccggct ggtcactgcc
     3001 gactgaggtg ccggccgaac tcgaggggca ggtgatcgag caagtgaaga tgaagtacct
     3061 ggccaccgat cgggaccccg cgctcaggtc cgagaacacg cccgacgtct ggtccggctc
     3121 gtatgccgtc gccggcggcg acagcatcgg cgagagcggc ctgctgaagt cactggaagc
     3181 ggcgctgtcc ccgttcaaga cgtgggcggt gtgatggcgg ctttggatgt cgtccgcaac
     3241 gctgctcgcc tgatcgcgct gcacggcgag acgatggtgc tgaagcgggc gagcgaagct
     3301 acgaccatta cgctgaaggg caaacgcctc gtcggttcga cggtcgatat aggcggctcg
     3361 gccgtccagc aggagttccg ggtgaagatc ggcccgtcgg aactgtcatc gtcggcctgg
     3421 acgagcaagg caccggttcg ccacgacagc atcgtcatcg gcgaccgcga aaggtcgatc
     3481 ctcgatgtcc gtcccttggg cgatgggggc acggtcgcac tctacgagtt gctggtcgcg
     3541 ggctgacatg cccgtcattg tcgagggcat cacgaccgag caactcggcc gcagcattgc
     3601 cgactgggtc aaggctgcca ccatacaaac cgccgagcgc gcattgcgtg aggaggtggc
     3661 cagaggcttc gacaccgagc cggtggtcat caccgacggc atgccgcgac gcgactatct
     3721 gcaggtaaag ccgttcggca ggatcgagtt cgcggcgcgc accagcatgg tggaggcggt
     3781 tcgctgggcg ctgaccgaat tgcagaataa gagcccggtg ctgacggggc gatatgccag
     3841 ttcgcacacc gtgatgatca acggtgccga ggtagagggc aatatctggg ttgcgctgcg
     3901 caacgctcgg ccgacggatc gcgtccagat cgtcaatccg cagccctacg cccgaaagat
     3961 cgagggcgcg acggccaata gaagaacggg gcgcggcaag cgggcggccc tcagtcggca
     4021 ggccaggagc ggcgtctatc gcgtcgtgct gcgggccctg gtcaaccggt tcggcaaggc
     4081 gctgttcttc gacttcaaat acgtgaacct gaacatcggc atcaaagtgt ggggcaaaca
     4141 gggcgcccgg cgcgtccagc gggaccaggt gtatccagcg ctgcagttct tcatcaagcc
     4201 cacggggttg cccaactaag gtcaggacat gatgtatggc gttcatccga tcgccagcgt
     4261 gccgctgggc gcgacggcca cgggcctggt tgttggcgac cccctgcgca tggcgttccg
     4321 cgaccggctc atcgtctggt tggctgacct cggtatcggc tggccgatca gggatctcta
     4381 caacacgggt gaaaatccgg acgtctcgca gggctttgtg gcgctcgatt ttccgggtgg
     4441 caccgaggat cagtacacgt tcggcgcgcc aggactgaac ttctggcgcg agcagggtca
     4501 ggtgacgctc tacgtgaaga cgcggctcgg cgccggcatg acgatgcgca atctggccga
     4561 gtcctacgct ggtgccctgc ggtcccggtt ccgcaacgac cgcttcgccg ccggcaacgg
     4621 cgccgttcgc atcacctcga ccgggcccat gggcggcggg cacgacgagg cgggcctctg
     4681 ggtggaagcc gtggccctag ggtacgagac tttcaacatc ggctaagccg gcagagcacg
     4741 ccgccagccg cacctgaccg ccgccccatg ggcggctttt tgttgtctgg aacttgggag
     4801 aagacctctt ggacagcgcc aacaagcaga ccgccgtcat cgccgaagcg accgtcggca
     4861 ccacgccggc gacgccggct ttcctgctga cacgcgacat tcgcgtcagc ggcgcgccac
     4921 aacgccccaa tacccgctcg cccgagcggc gctccgaccg ccaggcggcc tcgatggtgc
     4981 agggtgtcgc gacctatccc aagcagatcg agatgccctg ggtccgggac gcggccagcg
     5041 acgttctgtg ggcctcgctc ttctgcagcc cctgggtgtc caatgttctc aaagtcggct
     5101 cggtgcgaag cggcacgacg ttcacgctgg aagagaagta cgagggcggc gccaccgatc
     5161 cctatcgtcg cctgaccggc tgcatggtcg acaatgtctc gatcgccttc cagaacggca
     5221 acccgggcca gatcaccttc tccctgttgg gcctgggcga gaccacggcg acgacggcga
     5281 tcgcatcatc gacctatgcg gcgccgacgc cggcctacga tccctcgacg ccggcggaca
     5341 tcgtggtcaa cagcctgttc ggccttgcct cgccgaaggt ccgctcgctg caactgcaga
     5401 tctcgaacaa cctgcagcag cagtacgcct tcggttcggc cgatcccttc gccattggcc
     5461 tcggcgagtt caacgtgcag ggcgtggtcg aggtctactt caacgccctt gccgactact
     5521 cggccttcgt cgtgaagcag actggccaga ccgtcgatat cacgatcggc gccacaatga
     5581 acttcaagga ccggctcgtg ctgggcaatt gcgacgtctt caacccgaac gtcgatgatc
     5641 cgggccagac cggcgtccac gcggtcaccc tgaacttcct cggcaagtac tacgccaccg
     5701 acgcctcggc gatgaagctc acccgcaacg cgccgtaatg gcggcactgg agccccacat
     5761 ggaaacttcc gtcctcattc tccagacctt ccaccgcttc gtgtccgaag gcgagggcag
     5821 gcccgagcgc aaggaacgct ttgcccaggg ccagacggtc aaggtgtccg aggaggacgc
     5881 tgccgactgg atcgccaagg gcctcgcgcg agaagtctga atcgcgaaaa cccccaggtc
     5941 ccagctgcat ccccttgttc aggagtaaga catgagccag ttcaagtttg gcaacatcga
     6001 caagctgaag cgcaaccgcg agatcgaagg cgagagcggc accgagatcg gcctgcccgg
     6061 cgggatctcg ctgatcgcgc tctgcgcctc ggacgccaat ccggcctggc ggcgcggcgg
     6121 cgaggatttt ctggccgagc tgaagcggct gagtcgcgcc cacgcctcgg acgagcgcgt
     6181 gaagcgcttc ctggccgagc agcttgcccg catgctggtg aaggactgga gcggtgtcgt
     6241 cgaccaggac ggcaacgcca tcccgttcag catcgatgcg tgcatggagt ttctgatcga
     6301 ggctgacgac gcgatccccg ctttgcaggc agtggtctac gagacgcaga acttccgcgg
     6361 ccagcgtatc gaagcgatcg tcgaccacgc aaaaaactga tccgctggga cagctccaac
     6421 gcatccgaga tcgcgggctg gagcgatcgc gcccgagcgg gcgatgccga gttcgtcgac
     6481 cgcctgctgt cccggccgca acccgacatc gacacggcgc cctactggga agccttccag
     6541 tatctctccc gggaccgaac catgctgtct ctctccctgg gtatgggcgg cggcctggtg
     6601 ctgccgcagc cgatcccgcg tgaggcgatc cggcggtacg gcagggccct tcgtctctca
     6661 ggcgaggagc tggccgactt cttggagatc gtctccggca tcgacgactt ctgggtcgtg
     6721 accgagcagc gcaagcagac ggatgcagcg gttcgcgctg ccaggggcat gagcaggcaa
     6781 cgctgatcca tggctgaaga aaccaagatc atccgcctcg tcatcgacag ctcgaaggcc
     6841 gtcgacggcg ggcgggccgc gcagagggcg ctggaacaga tagagcgtgg cacatcctcc
     6901 ctggacagca ccatggcgcg catggagcag tcgctggggc gcgtcggcgg catgatcaag
     6961 gcgcatctcg ccctcatgct cgccgagatg gcggcgcgcc tcgtcgagac ggcgaaggcc
     7021 gcggtagagg cggtgggcgg cctggacgaa ctggccgagc agctgggcct cacgaccaaa
     7081 gggctacagg cgcttcagtt ctcgggcatt cagaacggtc tcaagctgga gcagctggag
     7141 actggcgctg ccaagttcag ccagaagatc ggcgaggccg ccaacggttc gaaggagatg
     7201 atcgaggccc tcgatcatct gggcgtcagg atactcgacg tcgacaagac cctgcggccg
     7261 accgaggaca tcatgagcga tgtcgcccgg gcgatcctgg ccatcgaaga ccccgcccgg
     7321 cgcgcggcag cgtcggtcga gttcttcggc aaggcgggga cgcggttcca acccgtgctg
     7381 caggatatcg ccaggggttt tggcttcatg ggcgaggaag cccgcaaagg cggggctctg
     7441 gtcgacgagc acgtggtcaa gcgactcgat cagctgtccg atgccttcga gcgcggcaag
     7501 ctccgctcgc gggcgttctt tgccgagatg gcggtcgcgg ttctcgacat ggccgacaag
     7561 gccgtcgatg cctaccgcaa gatcctggcg cagcaggcca cgcgcagttc gggtggcggc
     7621 taccagtggg agcgtgactt ccagtcctgg gtggagaacg ccgtgtcgaa catggcgggc
     7681 gtaggcgcgg gcgtcgtcga agtcttccgg ggagcggccg agcaggtggt taaactttgg
     7741 atcgacgcct tcaaccgcgt gctcgccggc gccgagcagg tgctgaacaa gctgaaagag
     7801 ttctctacgt tcggcctggg tgatgcgatc gcactcggcc ggatcccggg tggcggtgcc
     7861 agcgtcaacg attttgtcgg gggcatcacg caggcggccg agacggcgcg cgccgacacg
     7921 caggcctact acgctcgcca gcgcagtcca ttcggcaagg cttccagcgg cgtggcctgg
     7981 gcggtcgtca acgatggcct gccggcgcag gacgggcccc ggccgggcat cttcaatcct
     8041 ccggtcaaac ccggcggcgg cggctcggaa gattcgatcg acaagctgaa gcgtcagact
     8101 gaactcgcga tgcaggcggc gcaggcgctc gccgacgtct cggcccaggg cagccgggcc
     8161 gttgccgacc tcgagctccg tttgaaggca gtcaaggagg ttcaggacgc ctacgccagc
     8221 tcggcgcaca aggcgggtct ctcggtcgag gagctgacga agaggataga agatcagcag
     8281 cgtgccaccg agcgcgccaa gaacctgaag gacttcaaca tcgagacgtc cagcctgcag
     8341 aaggccaatg agcttctgga ggccgagaac cgtctgatcg atcagtcgac cgagacgcgc
     8401 gccgtcgtca tcgcccagat caggctgaag caggaactcg agctgaaggg cattggcgtc
     8461 gcgaccgagg ccgagcgggc ggccattgca gcgcgcggcg aagcgatcga gcaaaacgag
     8521 aggctgaaag gccaggccga ggagctgaag cgcgccaacg aactctggac cgaaccgctg
     8581 aaacaggcgt tccgcaacat ccagacggct ggcgccgatg cctgggaccg gatcctgcag
     8641 agcggccagg tgtcgttcca ggccctgggc gacatcttca agacgacgat ccggcgcatg
     8701 gtggctgagt ttttggccct cgcgacgatc cggcccgtga tgagcgtcgt ggtccagggc
     8761 ctgggcagcc tcggcgtggt ctcgccggcg gtggcccagc agatgggctt tggtggtgct
     8821 acgtcgggca gtgggagcct gggaggcggc agtattcccc tcccgtcgct tggcagcacc
     8881 ggtggtggca gcttctttag cggccttggc ttgggcggca tcggtgactg gctcaacacg
     8941 cccttcaccg ggccctacgc cgggatatcg ccgtcggcga tgcagggcgt tccaacgctg
     9001 tcgcccagca tgtggaaccc ttcgacctgg agcattacgc ctctgcaggg tatcggcgcc
     9061 gccgctggta tcggcatggg cgtctatcag ctggcgaact ccaggagcac cggccagacc
     9121 atcgctggca tcggcagcat ggtcggcggt gccgtgtcgc tgataccggg cctgcagccg
     9181 gtcggcatgg cgatttcgct gctgtcccag ttcgcacccg ccatcttcgg cgagtccaac
     9241 acccggacgc acagttcgac gaacgcctcg ctgcactacg gcaacggcaa ctggtacacg
     9301 accggcggtg catatggccc gaacgccaat agcggccagt cggaaagcgc gctgcgcggc
     9361 accacagcgg gcatcgatgc ggtgctgggc atcctgggcg gggtgaagga cccatcgaag
     9421 gtctgggggc tcaacgcctc atcctggacc gcccagggca aggactggag ctataccagc
     9481 aacgccacgc acctcgtcga cccggagacg ggcagccagg aagcctggcg gatgaacatg
     9541 gacgacatga tggacaccgg cgccgcccag gtggcgatcc ggtcgatcct gtccggcgcc
     9601 gtcggcgaga tctcggcgac gatgaagacc gcgctggagt ccatgcgcgc cgcttccatg
     9661 ggcatcaagg agaccgcgga gagcattgtc ttcgtcgatg acgtctatga gcggctcggc
     9721 aagggcgcgc tggctgtccg gacgcagttc cgcgagctcg agaagcagtt cggcgacatg
     9781 accgacaagg cgaccaggct cgggctcgcc ctggcgccga tcgaggcgga gcagaagaag
     9841 gccacggaac gcctcggcca ggactatgtc gacaacctga tcgatccggt cgcggccggt
     9901 ctgcgcgcct gggaagacga gaagcagtcg attctggcga atatcgacta tatccaccag
     9961 cacaccgacg tgatcgtcga ccaggcccgc atcgccgagg ccctgctgcg caaggaggcc
    10021 gcgctcaagg agcagctcta tggcggcgcg atcttgcagc tggaggacgc gatccggcgt
    10081 ctgagcctgg gcgacctcgc caacctatcg cccaccacca tgctgacggg cgtgcgagcg
    10141 gcctaccagg ccaccgtcgc gcaggcccgt tcgggtgaca gcagcgccat cgcgcgcgtc
    10201 gcggccgagg gtacgacctt cgcgcaggcg gcccagtcct actatgcgag cggtcctgac
    10261 tacgaggcgc tgaagcagca gatcctcaac gatctgctga ccctgcagtc gcagatcgcc
    10321 ggcagcggtg gcacgaccgg ctcggcgatg aacgacaaca atggcgccac gcaagcggcc
    10381 gtggcccagg cgacccagct tcaggctgtc gtctccgaac aaagccggca gatcgcagcc
    10441 ctgatggcga agctctccga gaccaacgac ctgttgctgc gccgcgcggt aaacggctga
    10501 gggggcgact acatggccag cacgatctac ttgacgggct tgcccggccc ctccgggaca
    10561 gtcgactatc ccgcgttgac gcagttcccg gtcgacgtgg gcgccgatgc cgtcgacctg
    10621 cagtcggcga gagggctttc ctaccttttg cgggccgcac cacctgatcc cggtggcaat
    10681 atcgttctga gcggtggcgc gccctatccc tcgggcatga ccgattggcc gacgcccttc
    10741 caggtc
//
LOCUS       DAOMEO010000003         9998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1149_length_9998_cov_4.603741, whole genome shotgun sequence.
ACCESSION   DAOMEO010000003 DAOMEO010000000
VERSION     DAOMEO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1149_length_9998_cov_4.603741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>2604
                     /gene="traA"
                     /locus_tag="PLH31_00150"
     CDS             73..>2604
                     /gene="traA"
                     /locus_tag="PLH31_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HQR87701.1"
                     /translation="MAIYHFSVKIISRNDGRSAVAAAAYRAATALHDLKIGRTHNFSN
                     KTGVVHSEILAPSGSPARWADRETLWNEVEKRERRDDAQLSREVEFALPRELDQAQGV
                     ALARAFVQREFVDLGMVADLNVHWDFDELGQAKPHVHVMLSLREVGPEGFGLKVREWN
                     ARGLLETWRESWANHVNERLAELDIDVRVDHRSLEAQGLGLVPQNKIGPVDARTYAAG
                     ETPFKAEEHRKIARCNGERLIAEPDLALEAITQQQSTFTERDLQRLAHRQSDDRTQFE
                     EVVRAVNASPNLIALGKDGAGRERYTSRAMVEVERRLEQDAAGLAGLKHYRVSPAASL
                     KARQSAEVTMSQTQEAAFAHVLGPEALALVVGYAGSGKSTMLGAARLAWTEQGYRVRG
                     AALSGMSAEGLQSGSGIASRTIASLEHAWAQGRERLVSGDVLVIDEAGLIGSRQMQRV
                     MAEASKAGAKVVLVGDAEQLQAIEAGAAFRALTERHGSVEITQIQRQREVWQQEATKA
                     LATGRTVQALEAYEAAGMVRGHATREAAGRALVEGWAKERLEPSAPSQIMLAHTRADV
                     AWLNGLAREALIKAAALGVGQSVVTDRGLRTFAEGDRVMFLRNERSLGVKNGSLATLV
                     SIAGDRAVVHLDKGNRRLEVDLKVYNHLDHGYAVTLHKSQGMTVDRAHLLATDGLDRH
                     AAYVGLSRHRQAVTMHYGADDFRNRNELVRTLGRDRSKDTTLDYAAAFAERRWLPAEP
                     IRIPKEIARATVQAARSTFAGFKPKPPAQVAESEVSSLDRAMIAYARAHADVRRMGQA
                     SLPHQAAALERTYGAVAALDPAQALDLRTALEERPSLAARAVEAGD"
     assembly_gap    2605..2614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2676..2936
                     /locus_tag="PLH31_00155"
     CDS             2676..2936
                     /locus_tag="PLH31_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87702.1"
                     /translation="MAQAFVTTWTQLHDDPARDPDVGGTMTKLIEGVESHRVQAVLCL
                     RWQELGLTKAPAAPADLRRALSAVVEKTRSAIEKDDGFELDL"
     gene            2948..3562
                     /locus_tag="PLH31_00160"
     CDS             2948..3562
                     /locus_tag="PLH31_00160"
                     /inference="COORDINATES: protein motif:HMM:NF039997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6118 family protein"
                     /protein_id="HQR87703.1"
                     /translation="MASEAEDPAAQAFEALRRDVASLRDDIKRIADPIDYSPTLQSIV
                     QRLEDLKKPALSRSPIADTAAHEAANFEQARLLSGLKQQISALEGVVGRARDRRQWWV
                     GIGLASLGGALIGAACCVALAGPIARTFPSVSYAMAKAALGQVRWDRGIRFMAHADDA
                     TWNQVATGLVLVQTNAAALDKCAKAASRGKSRTCQIQVPEPAER"
     gene            3648..3818
                     /locus_tag="PLH31_00165"
     CDS             3648..3818
                     /locus_tag="PLH31_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87704.1"
                     /translation="MKVPPTLWPAPDADWRLPADLIETIQQTITQPMPADHSKLQRHN
                     LGSPQGLLAKVR"
     gene            4221..6172
                     /gene="mrdA"
                     /locus_tag="PLH31_00170"
                     /pseudo
     CDS             4221..6172
                     /gene="mrdA"
                     /locus_tag="PLH31_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
     gene            complement(6331..6903)
                     /locus_tag="PLH31_00175"
     CDS             complement(6331..6903)
                     /locus_tag="PLH31_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQR87705.1"
                     /translation="MAANRRDGDILGYARVSTEDQDLTGQRQRLTEAGAIRTFEDIVS
                     GKRFDRPGLTHLLDHARAGDTLCVVRLDRLGRSLKELLETVDDLKRRDINFMSLEEKI
                     DTASAAGELIFHVFGAIAHFERRLIGERTRDGLAAAKARGARAGRPPLDQQKIAAGRA
                     LVESGMSPTAAARHLGLGRSTLYRELSRPK"
     gene            7177..>9998
                     /locus_tag="PLH31_00180"
     CDS             7177..>9998
                     /locus_tag="PLH31_00180"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HQR87706.1"
                     /translation="MTLREVARFYTLSPADLRWLEERRGPANKLGAAVQMALLRHPGF
                     GWSPGQTVPEIVLRFLSDQVHVAPEAMSEYSTRPPTRVEHASQLHQRLGLRLFVRGDL
                     RQAVSIAATAARATDKGGPIIEALIADLRRMGIILPSPDTLERVGLAGRSQARRKAAE
                     DLLAPISPSQLERIDQLLVNDATLKKSPLAWLRDTPESPSAASMVGITERLAYVRAMD
                     IDPAITETIHERRFEQYAREGAVAPAFLLSGYSVGRRRATVVAQLIFLESRLSDAAAD
                     MFDKMVGSLFAKGRRGRERKYQASSREVSQIMRLFSGVIGAVDQARVDGGDVLDRIDA
                     QVGWWKVLAAKPKVDALAALAIEDPLVSAADRYGALRRFVPTFLEHMRFKAGAGGAPL
                     LKALEILRDLNQSGRRELPSDAPLPFASKHWKALVKPVGSPINRRLYETAVAATLRDR
                     LRSGDVWIEGSRSYKRFADYLLPKDEVAAEAETLPVNVEMGTYLAERAGLLDQRLAAF
                     SKRLARDDLVGVTLKGEELSVTPVKANAPDEARSLDRAIDALLPRVRITELLREIDAL
                     TGFSSMFRELRSGKVHDNPSCILAAVLADATNLGLERMANASQGVTYAQLAWTQSWYL
                     SEENYKAALARIIEVHHAQPFARHWGDGQASSSDGQFFRSGRRRGGAGEVNAKYGPEP
                     GLRIYTHLSDMHGSFHTRVLSATSSEAPYVLDGLIGRGGGEHYTDTGGATDHVFALCH
                     LLGYRFVPRLRDLQDRRLAIIGAKPRHKILEPLLGRPIRVDVIEENWDDIVRLAASIK
                     AGSVAPSVMLKKLSAFKRQNRLDMALAEVGRIERTLFTLDWLESPELRRRCQAGLNKS
                     EARHALAQAVFVHKQGRIADRTLHNQEHRASGLNLVIAAIALWNTLYMQRAVEHLRER
                     EVAAPDELLAHLSPMSWAHVGLTGDYL"
BASE COUNT         1726 a         3167 c         3395 g         1700 t
ORIGIN      
        1 gcaccaaaat atattttgag tgcgcgctta tacattgctg tcgcaacgtg gctcgtgagg
       61 taaggtcctc aggtggccat ctatcacttc agcgtcaaga tcatcagccg taatgacggc
      121 cgcagcgccg tggcggcggc cgcctatcgc gcggcgacgg cgctgcatga tctgaagatc
      181 ggccgcactc acaacttcag caacaagacc ggcgtggttc attcggagat cttggcgccg
      241 tccggatcgc cggcccgctg ggcagaccgc gagaccctgt ggaacgaggt cgagaagcgt
      301 gagagacgcg acgatgcgca gctgtcgcgg gaggtggagt ttgctctgcc gcgcgagctt
      361 gatcaggcgc agggcgtggc tctggcgcgg gcgttcgtgc agcgcgagtt cgtcgaccta
      421 ggcatggtcg ccgatctgaa cgtacactgg gactttgacg aactgggcca ggccaaaccc
      481 catgtccacg tcatgctgag cctgcgggaa gtcgggcccg aggggtttgg tctgaaggtg
      541 cgggagtgga acgcccgggg gctgctggag acctggcgcg agagctgggc caaccacgtc
      601 aatgagcgcc tggctgagct cgacatcgat gtgcgggtcg atcaccgcag cctggaggct
      661 caaggtctgg gtcttgtgcc ccagaacaag atcggcccgg tcgatgcgcg gacctatgcg
      721 gccggcgaaa cgccgttcaa ggccgaagag caccgcaaga tcgctcgctg caatggcgag
      781 cggctgatcg ccgagccgga tctggcgctg gaggcaatca cccagcagca gagcaccttc
      841 actgagcggg acctgcagcg gctggcccat cgtcaaagcg atgatcggac ccagttcgag
      901 gaggtggtgc gggcggtcaa tgcctcgccc aacctgatcg cgctgggcaa ggatggggca
      961 gggcgcgagc gctacaccag ccgcgcgatg gtggaggtcg agcgccgcct tgagcaggac
     1021 gcggcgggtc tggccgggct caaacattac cgggtctcgc ctgcggcatc cctgaaggcg
     1081 cggcaaagcg cggaggtaac gatgagccag acccaggagg cggcgtttgc tcatgtcctg
     1141 gggcctgagg ctctggccct ggtggtgggt tatgccggat cgggcaagag caccatgctg
     1201 ggcgcggcgc ggctggcctg gacggagcaa ggctaccgcg tgcggggcgc ggccctgtcg
     1261 ggtatgtcgg ccgaggggct gcagagcggg tcgggtattg cctcacggac catcgccagt
     1321 ctcgaacacg cctgggcgca gggccgcgag cggctggtgt ctggcgatgt gctggtcatt
     1381 gacgaggcgg gtctgatcgg ctcgcgccag atgcagcggg tgatggccga ggccagcaag
     1441 gccggggcca aggtggtgct ggtgggcgac gccgagcagc tgcaggccat cgaggccggg
     1501 gcggcgttcc gggccctgac cgaacgccat ggctcggtgg agatcaccca gatccagcgt
     1561 cagcgcgagg tctggcagca ggaggcgacc aaggccctgg cgacggggcg cacggtccag
     1621 gcgctggagg cctatgaggc ggctggcatg gtgcggggtc atgcgacgcg cgaggcggcg
     1681 ggccgggcac tggtggaggg ttgggccaag gagcggctag agccgtccgc gcccagtcag
     1741 atcatgttgg cccacacccg ggccgatgtg gcgtggctca atgggctggc gcgcgaggct
     1801 ttgattaagg ccgccgccct gggcgtgggc cagagcgtgg tcaccgatcg aggcctacgg
     1861 accttcgccg agggcgaccg ggtgatgttc ctgcgcaatg agcgctcgct gggggtcaag
     1921 aacgggtctc tggcgacctt ggtctcgatc gccggcgacc gggcggttgt gcatctggac
     1981 aagggaaacc gccgtctgga ggtcgacctg aaggtctaca atcacctcga ccacggctat
     2041 gcggtgaccc tccacaagag ccagggcatg accgtcgacc gggcccacct gctcgcgacc
     2101 gacgggctgg atcgtcatgc cgcctatgtg gggctgagcc ggcatcgcca ggcggtgacg
     2161 atgcattatg gcgctgacga cttccgcaac cgtaatgagc tggtgcggac cctgggccgc
     2221 gatcgctcca aggacactac tctggactat gccgcagcgt tcgccgagcg gcgctggctg
     2281 ccggccgagc cgatccgcat ccccaaggag atagcccgcg cgacggttca ggccgcgcgg
     2341 tcgacgtttg ctggatttaa gcccaagcct cccgcccagg tcgccgaaag tgaggtctca
     2401 agcctcgacc gggccatgat cgcttatgcc cgggctcatg ccgatgttcg gcgcatgggc
     2461 caggccagcc tgcctcatca ggctgcggcg ctggagcgga cctatggcgc ggtcgcggcg
     2521 ctcgatccgg cacaggcttt ggatctgcgt accgctctgg aagagcggcc ttctctggct
     2581 gctcgagccg tcgaggcggg ggacnnnnnn nnnncgccag cccgttgcgc caggctgcgg
     2641 agtctgaagc ctcccttcgc ggggccatag accagatggc gcaggcgttc gtgacgactt
     2701 ggacgcaact gcacgacgat cctgctcgtg atcctgacgt cggcgggacg atgaccaagt
     2761 tgatcgaggg cgttgaaagc catcgtgtgc aggccgtcct ttgtctgcgt tggcaagagc
     2821 ttggcctgac caaagccccc gccgcgccgg ccgatcttcg ccgagcgtta agcgccgtcg
     2881 tggaaaagac caggtccgcg atcgaaaagg acgatggctt tgagcttgat ctgtaaggag
     2941 cccagctttg gcgtctgaag ccgaggatcc cgccgcccag gcctttgagg ccctgagacg
     3001 ggatgttgcg tccctgcggg atgacatcaa gcggatcgct gatccgatcg attacagtcc
     3061 gacgctgcag agcattgttc agcgcctgga ggatctgaaa aagcccgccc tcagtcgatc
     3121 gcccatagcc gataccgcgg cgcacgaagc cgcaaacttt gagcaggctc ggttgttgtc
     3181 gggcttaaag cagcagatca gcgcccttga gggcgtggtc gggcgggcgc gtgatcggcg
     3241 ccaatggtgg gttgggatcg gcctggcatc gcttggcggc gcgctgatcg gggcggcgtg
     3301 ctgcgttgcg ttggctgggc caatcgctcg aaccttcccc agtgtgtcct acgccatggc
     3361 gaaagccgcc cttgggcagg tgcgctggga tcgtgggatc aggttcatgg cccatgcgga
     3421 tgatgcgacc tggaaccagg tcgccaccgg gctggtgctc gttcagacca atgccgcagc
     3481 cctggacaag tgcgctaagg ccgctagtcg gggcaagtcg aggacctgtc agattcaagt
     3541 gccggagcct gcagagcgat aggtcggtga gctgaggccg gcgtcggagg gaagggagaa
     3601 ggggaggggg ggggagagcg caatcgcgct tttgaaagga ccctccgatg aaagttcctc
     3661 ccaccctgtg gcctgctccc gacgcggact ggcgcctgcc ggccgatctg atcgagacca
     3721 tccagcaaac tattacccag ccgatgccgg cggaccattc caaactgcaa cgccacaatc
     3781 tgggctcacc tcaaggtttg ctcgctaaag tacgctaagc agaacgtata aggaacgggc
     3841 tggacgtcag cgccctcagt cggcaccgtt tctgcggatg cttgaacggg ttggatgggt
     3901 tggatgggtt ggtgagccct aggtgcaggt agctgcagta tgcctgtggc gggtcggccg
     3961 gcgggccatg ttcttgcagc gatgcgagtg tccggccacg ctcttgagcg gacatcgttc
     4021 acgcttcttg atcacggaaa cgttgctcgg cgtatttatc tgaaccattg acgcgggtgc
     4081 gcgcgggccc actgtaggct gagaccaacc cctatttgcg gccgttaccc ttccatgatt
     4141 tccctggccg ccgcgacgtt gtcgcgccct cgagcgatgc cgctcgagcc ctccgtcacg
     4201 aagttggcgg agcaagcgcg atgagcgagc caacgatctt tttctccgag gtcaacgaac
     4261 gccagggcgc cttccatcgc cgggcttttg tgatgggcgc cttcgccggc ggcggccttc
     4321 tcgccctagg cggacggctg gcccagctgc aattggtcga cgccgcgcgc taccagaagc
     4381 tgtctgcggg caaccaattc aatttccgcc tagtctcgcc accgcgcggc atgatccgcg
     4441 accgcaacgg cgtcgccttg gcttcaaacc gccccaattt tcgccttctc gtcaccaagg
     4501 acaagggctt cgacgccgag gcgactttga aggacttagc ccagctggtc ccgatcgacg
     4561 acgcgcgtcg cgcccgcatt ctcaaggacc tgacccatgc gccgaagaag gcgcccgtgg
     4621 cggtgatgga ggacatgact tgggaggagt tctcccgagt aaatatccgc gcgcccgagc
     4681 tgccgggcgt caccgccgac atgggcgagg tgcgggtcta tccgttcggc ggggccttcg
     4741 cccacgtcat tggctacgtc gccaaggttt ccgaccggga tttggagaag gccaaggacg
     4801 atcccaacca ggaccagacg ctgctgcata accccggctt ccggatcggc aagctgggcg
     4861 tggagaaagc cttcgacgtg ccgcttcgcg gcaaggccgg tgccaccaag gccgaggtcg
     4921 acgcccaggg gcgcaccgtc cgcctggatc ccgagggcga catcccggcc aagcccggcg
     4981 ccgacattct cttgaccctc gacgccgaca tccagaaccg cgccctggag gttatgggcg
     5041 acgagagcgg cgcgatcgtg gtgatggact gccgcaacgg cgacttgctg gccatggcct
     5101 cggcccccag cttcgacgcc aaccgcttcg tcaaaggcct gtcggggccg gagtacaaag
     5161 ccttggccga gtatgagcgc aagccgctgc tggacaaggt gatgactggc acctatccgc
     5221 cgggctcgac gttcaagccg accgtggcca tggcggccct gacggctggc gtcaatccta
     5281 gcgagcgtgt ggtctgcacc ggcggctggg cgttcggcgg tcgggtctgg cactgccaca
     5341 agaagggcgg ccatggcgcc caggatatgc acgcggcgat caagaacagt tgtgacgtct
     5401 atttctacca gatggcgctg cgcatcggcc ccgacccgat tgccgcgacg gccaaggctt
     5461 tcggctatgg cgggaccttc gacattggca ttcccggtca gaagaagggg ctagtgggct
     5521 cgcgcgagtg gaagaagacc gccttcaagc gcgacccgac ctggcatccg ggcgacagcg
     5581 tgccctacgg aattggccag ggttatatca acgtcaacgc cctgcagctg gcggtgatga
     5641 ccgcgcgcct ggccaatggc cgaaaggcgc tgaacccgag gctggtcaag tcggtgggcg
     5701 gagtcgaaca gccgcatggc gacgcggtgc ccgacctgcc gttcagccag gcgcacctgg
     5761 agatcgtccg cgccggcatg gcggccgtcg ccaacgatcc gaccggcgga gccttcaaac
     5821 agagccagct gggcctgggc gacgtcaaga tggcgggcaa gaccggcacc gcccaggtcc
     5881 gccagtacgc ggcgggcgcc tcgcgcagca accagggcgt ggcctggcgc ctaaaggacc
     5941 acaatctgtt catcgccttc gcgccgtacg ataatccccg ctacccgcat cacctcgcga
     6001 gcacggcggc cagttcggct ccagcgccgc cgccccccgt gctcgcgagg tgatgcgggt
     6061 agctttgctg aaggatccgg aaatccgcga gcggatcata aggccgatgc ctctgcctga
     6121 agcccccgac gaaagcgaca tcgatcctga agctccgaca gccgacacgt aggcgtccgg
     6181 gaccactctc tcagagggtg aggcggtcgc tatggattcg gcgctgcgcg gccgccgcac
     6241 tggcgctatg ggcggcgtcg ccgtgcccgt gaacgcgcga aaggcagcga accgacaacc
     6301 gtgcggcggc ccgctcaggc ccggcgatcg tcatttaggg cggctgagtt ctcggtaaag
     6361 cgtggagcgg ccaagcccca aatgccgtgc tgctgcggtt ggcgacatac cgctctcgac
     6421 gagggcacgg ccggcggcga tcttctgttg gtctagcgga gggcgaccgg ctctcgcgcc
     6481 gcgagctttg gccgccgcga ggccgtcacg ggtgcgttca ccgattaggc gcctctcaaa
     6541 atgcgcgatg gcgccgaaca catggaagat cagctcgccg gccgctgagg cggtgtcgat
     6601 cttctcctcc aagctcatga aattgatgtc tcggcgcttt aggtcatcga cggtctccag
     6661 cagttccttc agcgaccggc ctaggcgatc gagccgcacg acgcagagcg tgtcccccgc
     6721 gcgggcatgg tcgaggaggt gggtaaggcc gggccggtcg aaccgcttgc ccgaaacgat
     6781 gtcttcaaag gtgcggatcg cgccggcctc agtaagccgc tggcgttgac cggtcagatc
     6841 ctgatcttcg gtcgacaccc gagcatagcc caagatgtcg ccgtcacgcc tgttcgccgc
     6901 catcgctcgt ctccaaaacg gtcggtttat ggacaccggg ccaacccgta ccaaaggtgg
     6961 cccggcggcg tccataaata ccgtctctat attcggcttc gtcccctaat caaatagacg
     7021 gtttgtggac gaatcgaagc ggctgaattt tagaacggct ggtcaggctc tggtttttcc
     7081 agaattgtga tccgtcgcag aagcggcgga gaccatccca tgacgcggcg aagcttgctt
     7141 acgggtgatg agaggcgaaa gctgtttgag ccgccgttga cgttgcgtga ggtggcgcgc
     7201 ttctacacgc tctcgcccgc cgaccttcga tggctcgaag agcgccgagg gccggccaac
     7261 aagctagggg ccgccgtgca gatggcgctg ctgcgtcatc cagggtttgg atggtcgccg
     7321 ggccagacgg tcccagaaat cgtgctgcgt ttcctctccg accaggttca cgtcgcgccg
     7381 gaagcgatga gtgagtactc gacgcgccca ccaacgcgcg tcgagcacgc cagccagctg
     7441 catcagcgat tgggactgcg cctgtttgtt cgcggcgatc ttcgccaagc ggtgtcgatc
     7501 gccgctacgg cggcccgcgc gaccgacaag ggcggaccga tcattgaggc cctgatcgcc
     7561 gatctgcgcc ggatggggat aatcttgccg tcacccgaca ccctggagcg tgtcggttta
     7621 gccggccggt ctcaggctag acgcaaggcg gcagaggatc tgctggcgcc gatctcaccc
     7681 tcgcaactgg agcgcatcga ccaactgttg gtcaacgacg cgaccctcaa aaagtcgccc
     7741 ctggcttggc tacgcgatac ccccgagtcg cccagtgccg catcaatggt ggggatcacc
     7801 gaacgcttag cctacgttcg ggccatggac atcgatccgg cgattaccga gaccatccac
     7861 gaaaggcggt tcgagcaata tgcccgcgaa ggcgccgtcg ccccggcgtt cctgctcagc
     7921 ggctacagcg tcgggcgccg gcgggcgacc gtggtggcgc agctgatctt tctcgaaagc
     7981 cgcctcagcg acgctgcggc cgatatgttc gataagatgg tcggctcgct gttcgccaag
     8041 ggccgccgcg gccgcgagcg caagtaccag gccagtagcc gcgaggtcag ccagatcatg
     8101 cgcctgttca gcggcgtgat cggcgccgtt gatcaggccc gggtcgatgg cggcgacgtg
     8161 ctcgaccgga ttgacgccca ggtcggctgg tggaaggtgc tggccgccaa gcccaaggtc
     8221 gacgccttag cggccctggc gatcgaggat ccgctggtca gtgccgccga ccgctacggg
     8281 gcgctgcgcc gcttcgtccc gaccttcctg gaacacatgc ggttcaaggc cggggccggt
     8341 ggcgcgcctt tgctcaaggc gttggagatc ctgcgcgacc tcaaccagtc cggccgacgg
     8401 gagttgccca gcgacgcgcc gctgccgttc gcgtccaagc actggaaggc tttggtcaaa
     8461 ccggtcggca gcccgatcaa caggcgactc tatgagacgg cggtggccgc cacgctgcgc
     8521 gatcgtcttc ggtcgggcga tgtctggatc gagggctctc gctcctacaa gcgctttgcc
     8581 gactatctgc tgcccaagga cgaggtcgcc gccgaagccg aaaccctgcc ggtcaatgtc
     8641 gagatgggaa cctacttggc tgaacgggcc ggcctcttgg atcagcggct ggcggcgttc
     8701 tccaagcgac tggccaggga tgatctggtc ggcgtgacgc tgaagggcga ggagctgtcc
     8761 gtcacgccgg tcaaggccaa cgctccggac gaagcgcggt cgctagaccg ggcgatcgat
     8821 gcgctgctgc cacgcgtgcg gatcaccgaa ctcctgcgcg agatcgacgc cctgaccggg
     8881 ttctcgtcga tgttccgcga actgcggtca ggcaaggtgc acgacaaccc cagctgcatc
     8941 ttggccgccg tcctggccga cgccaccaat ctgggccttg agcgcatggc caacgccagc
     9001 cagggtgtca cctacgcgca gttggcctgg acccagagct ggtatctgag cgaggagaac
     9061 tacaaggctg cgctggccag gatcatcgag gtccatcacg cgcagccgtt tgccaggcac
     9121 tggggcgatg gccaggcctc gtcgtccgac ggacagttct tcaggtccgg ccgtcgacgc
     9181 ggcggagccg gcgaggtcaa cgccaagtat gggcccgagc ccggtttgcg gatctacacc
     9241 cacctgtcgg acatgcacgg ctcgttccac acccgcgtgt tgtcggccac ttcatcggag
     9301 gctccctatg tgctcgacgg cctgatcggt cgcggcggcg gggagcacta caccgatacc
     9361 ggcggcgcca ccgaccacgt cttcgcccta tgccacctcc tcggctaccg gttcgtgccc
     9421 cgcctccgcg atctgcagga tcgtcggctg gccatcatcg gcgccaagcc acgtcacaag
     9481 atcctcgagc ctctgctagg ccgccccatc cgggtcgatg tgatcgagga aaattgggac
     9541 gacatcgtcc gtctggccgc gtcgatcaag gccggctccg tcgccccgtc tgtgatgctc
     9601 aagaagctgt cggccttcaa gcggcagaac cggttggaca tggccctggc cgaagtcggc
     9661 cgcattgagc ggaccctgtt cacgctggac tggcttgaga gccccgagtt gcgccggcgt
     9721 tgccaggcgg ggctcaacaa gagcgaagcc cgccacgcgc tggcccaggc tgtgttcgtt
     9781 cacaagcaag gccggatcgc cgatcgcacc ctgcacaacc aggagcaccg cgcctcgggg
     9841 ctgaacctgg tgatcgccgc catcgcgctc tggaacacat tatatatgca gcgcgccgtc
     9901 gagcatctgc gcgagcggga ggtggcggcg cccgatgagc ttctggcgca cctttcaccg
     9961 atgagctggg cgcacgtcgg cttgacgggc gactacct
//
LOCUS       DAOMEO010000004         9772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1171_length_9772_cov_2.468766, whole genome shotgun sequence.
ACCESSION   DAOMEO010000004 DAOMEO010000000
VERSION     DAOMEO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_9772_cov_2.468766"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>507)
                     /locus_tag="PLH31_00185"
     CDS             complement(<1..>507)
                     /locus_tag="PLH31_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQR87707.1"
                     /translation="VYAEQILVHALKSALLLDCSTIDVESARAVAAQARAAGFRFADA
                     PVSGGIMAAEAGTLAFMVGCDAADFPDVEAALAPMSRVTIHAGEHGAGQAAKICNNML
                     LGISMIGTCEAFALAEKLGLAADRFFEIASKSSGQCWSVSTYCPVPGVGPSTPADRGY
                     EGGFATAMM"
     assembly_gap    508..517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<518..666)
                     /locus_tag="PLH31_00190"
     CDS             complement(<518..666)
                     /locus_tag="PLH31_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQR87708.1"
                     /translation="MTRIAFIGLGNMGGGMAANQARAGRPVMAFDLSAQAVERAVAAG
                     CQAAGS"
     gene            complement(678..>1389)
                     /locus_tag="PLH31_00195"
     CDS             complement(678..>1389)
                     /locus_tag="PLH31_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR87709.1"
                     /translation="DQVVKDLSGAAFGSAGERCMALPVAVAVGKKTADELRERMVAEI
                     ETLKIGISSDPAAHYGPVVSAAHRNKIADYIRIGVEEGAELVVDGRDFSLQGFEKGFF
                     IGPSLFDGVKKGMKTYQEEIFGPVLQIVRAESFDEAIALPSEHQYGNGVAIFTRNGRA
                     AREFASRVNVGMVGINVPIPVPVAYHSFGGWKRSSFGDTNQYGAEGVRFYTKVKTVTA
                     RWPEGETEDSAFIIPTMK"
     assembly_gap    1390..1399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1400..2137)
                     /locus_tag="PLH31_00200"
     CDS             complement(<1400..2137)
                     /locus_tag="PLH31_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR87710.1"
                     /translation="MRAIDHFIAGASAPGASGRFGDVFNPNTGEVQARVQLATDAELD
                     AAVQNAARAQVDWAATNPQRRARVMFEFKRLIERDMNSLAEILSSEHGKVIADSKGDI
                     QRGLEVIEFACGIPQMLKGEYTDGAGPGIDVYSMRQPLGVCAGITPFNFPAMIPMWMF
                     GISIAVGNTFILKPSEKDPTVPVKLAELMMEAGAPAGVLNVVHGDKSVVDAILAHPLI
                     RAVSFVGSSDIAHYVYQTGTAHGKRVQA"
     gene            2206..3123
                     /locus_tag="PLH31_00205"
     CDS             2206..3123
                     /locus_tag="PLH31_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR87711.1"
                     /translation="MQAKSAGIQRVFEWDDLRYFLAAARAGSFSGGAQRTGVDTATVS
                     RRVARLETALKATLFMRSAAGLQLTSAGARLMEAGLKAEAAMQSVTGAADPEVSGGTV
                     RISAAEGFGAHVLARALPSFRLSRPTLRIELATTAGLLSPTRREVDLAVTLSAPTDSR
                     LLVEPLTDYQLAVYASPAYLAREGPFESAADLRRGQIVGYIDDLLHTPELRYLDDVAP
                     ALETALSSSSILAQREIIRAGGGAGVLPCFLSEGLERIAPEVLLKRRFWVSTLRDVAA
                     TARVRAVRAWLQDIAAARAHDLNPFASAD"
     gene            3229..>3706
                     /locus_tag="PLH31_00210"
     CDS             3229..>3706
                     /locus_tag="PLH31_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR87712.1"
                     /translation="MTDLASLRAQRQDLYHFIVRRTRDPAAADDLVQETLLRLMTYER
                     ARTVVDRAALGYRIALNLVRDHFRHRSRRAVETLDDDMPCEAPAPEQILMHRQKVEVF
                     GKALDAMPPLRRDVFIRRRLHGHSTRQIAAELAMNEAAVEKHVVRALEQLHREMAKA"
     assembly_gap    3707..3716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3717..4623
                     /locus_tag="PLH31_00215"
     CDS             <3717..4623
                     /locus_tag="PLH31_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQR87713.1"
                     /translation="AAVWVARRLDSQDLDQPGFDAWRSADPANGEAFDRLWATAQDAA
                     LTEAMALSVQRRAAWRLDWKPITALAGGLATCAVALVLAWPQLQLLTVRPVSVETRPG
                     QIRTVALVDGSRVTLDGATQLDVRIGSRRREVRLVRGEAFFDIAHDASRPFTVRAPEG
                     AARVLGTAFDLERGDGRLELSVHRGKVRLAPEGLIHPTVDLTPGQRAFTKDGRLSAVR
                     AFDPAADDWRSGWLETDSVTLARLIERLNRASPTPIVIDDPALGRRRVAGRFRLDEPE
                     TLVRNLALVHGFQVQERGGALRLSR"
     gene            4766..6312
                     /locus_tag="PLH31_00220"
                     /pseudo
     CDS             4766..6312
                     /locus_tag="PLH31_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516562.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     assembly_gap    6313..6322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6323..7655
                     /locus_tag="PLH31_00225"
     CDS             <6323..7655
                     /locus_tag="PLH31_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87714.1"
                     /translation="LPFRRIEFRTNDSTDEEIALQFDAERPVSLGPVSRIAFGVKYRE
                     RERDYNRADINYTTNLTGKFFGATYFEPFPVSDFLGDVDGTLPTTWAMPNPDAFWVLA
                     DKTQLGRARADQRNSYYVSEDIAAGYALADIDTSVMNLPLRGEVGVRAAQTKQVSAGH
                     ADNGRAAIPVRYVKTYSDVLPTANFVLDLSETLQARFAAAKVITRPSLADLAPRLTLN
                     SSGTVLTAVGGNPQLQPFEAWQYDATLEWYFAPGSALIAGAFYKDITTFVYNQKTNIV
                     VDGQTYQLTSPTNGGQATIKGVELAYQHLFKSLPAPFDGLGFLANYTYTDTEATYSPT
                     LKDEMVNVAKNSFNLTGFYEKDRFAARASYSWVDDVLQDVGGAGLSALNDSAFGSLDA
                     SVSYKITPQITAALEAQNLTNAAQWQFVKGGWFGGYTHYGRTVSLGLRAKF"
     gene            complement(7796..>8621)
                     /locus_tag="PLH31_00230"
     CDS             complement(7796..>8621)
                     /locus_tag="PLH31_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87715.1"
                     /translation="GTGPLPGATASESWHQQYNSLFSRNVTVATLTPFLPDPAKATGA
                     AVIVAPGGGFRTLSMENEGWDVARALADKGVAAFVLKYRLNQTPADMPGFERSMTEMF
                     SATARPPRPSPEAAMAGLAPQIADSRAAFALVRQRAAEWRVDPDRVGMIGFSAGAMLT
                     LATTLAGEDAKPAFIGLVYGPLTAVTVPAEAPPMFVALAADDPFFANSGFGLIDSWRA
                     AKRPVEFHLYEQGGHGFGMYQKTTTSTGWFDAYVRWIGMHGMLKPKGLAPNGAPAR"
     assembly_gap    8622..8631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8758..>9772)
                     /locus_tag="PLH31_00235"
     CDS             complement(8758..>9772)
                     /locus_tag="PLH31_00235"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQR87716.1"
                     /translation="TLTNGQGVSATIITYGATLQALIAPDRTGKTADIALGFADAAAY
                     ARNASYFGASVGRFANRIGKGRFTLDGKTYQLALNNNGVAALHGGVKGFDKVVWTVLD
                     AKSGPTASVTFGYLSPDGEEGYPGALNATATYALDEQNNLTVTYGATTDKPTIVNMTN
                     HALFNMAGEGSADGAMGNVLTIPADGYSPVDAELIPTGETTPVAGTAFDFREPTAVRA
                     RIRDASDPQMVMGRGYDHNYVLNGKSGGALRLAARLSDPKSGRVLEVLSDQPGLQVYA
                     GNFIDGTTVGKSGKIYRQGDGIALEPQHFPDAPNKSQFASTRLDPGQTYRNTMVFRLT
                     TTK"
BASE COUNT         1536 a         3303 c         3259 g         1624 t
ORIGIN      
        1 catcatggcg gtggcgaagc cgccctcgta gccgcgatcg gccggcgtcg aggggccaac
       61 accggggact gggcaatagg tcgagaccga ccagcattgg cccgacgact tgctggcgat
      121 ctcgaagaag cgatcggccg ccaggcccag cttttcggcc agggcgaagg cctcgcaggt
      181 cccgatcatc gaaatgccta gcaacatgtt gttgcagatc ttggccgcct gtcccgcgcc
      241 atgctctccg gcatgaatgg tcacgcgcga catcggggcc agcgcggcct cgacgtctgg
      301 aaagtcggcc gcatcgcagc ccaccatgaa ggccagggtc ccggcttccg cggccatgat
      361 cccccctgac acaggggcgt cggcgaagcg gaagcccgcc gcccgggcct gggcggccac
      421 cgcgcgggcg ctctcgacgt cgatggtcga gcagtccagc agcagggcgg acttgagcgc
      481 gtggaccagg atctgctcgg catagacnnn nnnnnnngaa cccgccgcct ggcagcccgc
      541 cgccacggcc cgctcgacag cctgggctga caggtcgaag gccatcaccg ggcgcccggc
      601 cctggcctga ttggcggcca tgccgccgcc catattaccc aggccgatga aggcgatgcg
      661 cgtcatggct gtggtcctta cttcatggtg ggaatgatga aggcggagtc ctcggtctcg
      721 ccttctggcc agcgggcggt gacggtcttg accttggtgt agaaccgcac gccttcggcg
      781 ccgtactggt tggtgtcgcc aaagctcgaa cgcttccagc cgccgaagct gtgataggcc
      841 accggcacgg ggatcggcac attgatgccg accatgccga cattgacgcg gctggcgaac
      901 tcgcgcgccg cgcggccatt gcgggtgaag atggccacgc cgttgccgta ctggtgctcc
      961 gacggcaggg cgatggcctc gtcgaagctc tcggcgcgga cgatctgaag caccgggccg
     1021 aagatctctt cctggtaggt cttcatgcct ttcttcacgc cgtcgaacag cgaggggccg
     1081 atgaagaagc ccttctcgaa gccctgcagg gagaagtcgc ggccgtcgac gaccagctcg
     1141 gcgccttcct cgacgccgat gcggatgtag tcggcgatct tgttgcggtg ggcggcgctg
     1201 accaccgggc catagtgggc ggccggatcg ctggagatgc cgatcttcag ggtctcgatc
     1261 tcggcgacca tccgctcgcg cagttcatcg gcggtcttct tgccgaccgc caccgcgacc
     1321 ggcagggcca tgcagcgttc gccggccgag ccgaaggccg cgcccgacag gtccttgacc
     1381 acctgatccn nnnnnnnnnc gcctggacgc gcttaccgtg ggccgtgccg gtctggtaga
     1441 catagtgggc aatatcggag ctgccgacaa agctgacggc cctgatcagc ggatgggcca
     1501 ggatcgcgtc gacgacgctc ttgtcgccat gcaccacatt gagcacgccg gccggagcgc
     1561 cggcttccat catcagctcg gccagcttca cgggaaccgt cggatccttc tccgacggct
     1621 tgaggatgaa ggtgttgccc acggcgatgc tgatgccgaa catccacatc gggatcatgg
     1681 ccgggaagtt gaacggggtg atgcccgcgc agacgcccag gggctggcgc atcgaataga
     1741 cgtcgatgcc gggaccggcg ccgtcggtat attcgccctt cagcatctgg gggatgccgc
     1801 aggcgaactc gatcacttcc aggccgcgct ggatgtcgcc cttggaatcg gcgatgacct
     1861 tgccgtgctc ggacgacagg atctcggcca ggctgttcat gtcccgctcg atcaggcgct
     1921 tgaactcgaa catcacccgc gcgcggcgct gggggttggt cgccgcccag tcgacctggg
     1981 ccctggccgc gttctgcacg gcggcgtcca gttcggcgtc tgtcgccagc tgaacccggg
     2041 cctgcacctc gccggtgttg ggattgaaga cgtcgccgaa gcggcccgaa gcgccgggcg
     2101 cgctcgcccc cgcgatgaaa tggtcaatgg ctcgcatcgg cgcgtctccg ttcagatctc
     2161 gcagaagaga atgcttgata ttcatgcaaa atagccattg caaatatgca agctaagtca
     2221 gcagggattc agcgcgtgtt tgagtgggac gatctccgct atttcctcgc cgccgcgcgg
     2281 gctggatcgt tcagcggcgg cgcgcagcga accggcgtcg atacggccac ggtaagccgg
     2341 cgggtggcgc ggctggaaac cgcgctgaag gccacgctgt tcatgcgctc ggcggcagga
     2401 ctgcaactga ccagcgcggg cgcgcgattg atggaggccg gtctgaaggc cgaggccgcc
     2461 atgcagtcgg tcacgggcgc ggccgatccc gaagtcagcg gcggcacggt ccggatcagc
     2521 gccgccgagg gttttggcgc ccacgttctg gcgcgggctc tgccaagctt ccgtctcagc
     2581 cggcccaccc tgcggatcga actggcgacc accgctggcc tgctgtcgcc cacgcgccgg
     2641 gaggtggacc tcgccgttac cctgagcgcc ccgaccgaca gccggctgct ggtcgagccc
     2701 ctgaccgact accagttggc cgtctatgcc tcgccggcct atctggcgag ggaaggcccc
     2761 ttcgaaagcg ccgctgacct gcgccgcggg cagatcgttg gctatatcga cgatctgctt
     2821 cacaccccgg agctgcgcta tctggatgat gtcgcgccgg cgctggagac cgcgctgtcg
     2881 agttcctcga tcctggccca gcgtgagatc atccgggcgg gcggtggggc gggcgtcctg
     2941 ccttgcttcc tgtcagaggg tctcgagcgg atcgcgccgg aggtgctgct caagcgccgc
     3001 ttctgggtca gcacgttgcg cgatgtcgcc gccaccgccc gcgttcgcgc ggtgcgcgcc
     3061 tggctgcagg atatcgcggc ggcccgcgca cacgacctca acccgtttgc gagcgccgat
     3121 tgaacgaaag cccgtcagaa ctgtcatcaa cgcatgctag cggccgctca tggttcatag
     3181 aggcgcgcct ggtcctgtcg gttctgcccc taatgcgggt gagcgcggat gaccgacctt
     3241 gccagcctgc gcgcccagcg ccaggacctc tatcatttca ttgtccgacg cacgcgcgat
     3301 ccagccgccg ccgatgatct ggtgcaggag acgctgctgc gcctgatgac ctatgagcgg
     3361 gcccgcacgg ttgttgaccg ggcggccctg ggctaccgga tcgcgctcaa tcttgtccgc
     3421 gatcatttcc gccatcgcag ccgccgagcg gtcgaaacgc tggacgacga catgccctgc
     3481 gaggcccccg caccggagca gatcctgatg catcgccaaa aggtcgaggt gttcggaaag
     3541 gcgctggacg ccatgccgcc cctccggcgt gacgtgttca tccggcgaag gctacatggc
     3601 cattccaccc gccagatcgc tgccgagctg gccatgaacg aggccgcggt cgagaagcac
     3661 gtggtgcggg cgctggaaca gctgcatcgc gagatggcca aggccgnnnn nnnnnnggcc
     3721 gctgtctggg tggcgcgccg cctggacagt caggatctcg accagccggg cttcgacgcc
     3781 tggcgatcag ccgatcccgc caatggcgaa gctttcgacc gcctctgggc gaccgcccag
     3841 gacgcggcct tgaccgaggc catggccctc agcgtccagc ggcgtgcggc gtggcgcctt
     3901 gactggaagc cgatcacggc cctggccggc ggcctggcga cttgcgcggt cgccctggtg
     3961 cttgcctggc cgcagcttca actgctgacc gtgcgtccgg tgagcgtcga gacccggccg
     4021 gggcagatcc ggaccgtggc cctggtcgac gggtcgcggg tcacgctgga cggtgcgacg
     4081 cagctggatg tgcggatcgg ctctcgccgg cgcgaggtgc gcctggtgcg cggcgaggcc
     4141 ttcttcgata tcgcccatga cgcctcgcgc cccttcacgg ttcgggcccc ggagggcgcc
     4201 gcgcgggtgc tgggtacggc cttcgacctg gaacgcggcg acggcaggct ggaactgtcg
     4261 gtgcatcgcg gcaaggtgcg cctggccccc gagggattga tccaccccac cgtcgacctc
     4321 acgccgggcc agcgagcctt cacgaaggat ggccgtctgt ccgcggttcg cgcctttgat
     4381 ccggcggctg acgactggcg ttcgggctgg ctggaaaccg acagcgtgac gctggcgcgg
     4441 ctgatcgagc gtctgaaccg cgcgtcaccg acgccgattg tcatcgatga tcctgcgctg
     4501 gggcgtcgac gcgtggccgg acggttccgg ctggacgagc ctgaaaccct tgtccgcaac
     4561 ctggccctcg ttcacggctt ccaggtccag gagcgcgggg gcgcattgag attgtcccgt
     4621 tgaccgtatc gggtgtcgtg tcctctcgcg gcacgaactc cggcgtcgcg gaaacttcac
     4681 aaaattcact gtcctggatt ggggtccagc aacgtcttca ccgccgaagc cggcgaacgc
     4741 gccgtgcgaa ttcggggatg acgcaatgac cttcaagacc tggacctggc tcggctcggc
     4801 gtcggcagcg gcgctgatga tggccgcccc agccctcgcg gcgcccgccg ccaatagcgt
     4861 cctggccatt ccggcgggcg acctggcttc ggccttgcca gccttctcgc gcgccacggg
     4921 cgttcaggtc ctggccgacc cggccctgct ggcgggcaag cgcaccggcg gcgtggcggg
     4981 cgcgctcaac gccgagaccg gcctgaccca gctgctgcgg ggcaccggtc tgaccttcgt
     5041 gcgcagcggc ggcgcggcca ttcttcgccc cgccgcccag aagatctcaa cggctgcgcg
     5101 cccggcgccg gccccgcgcg tcgagggcgc ggccgctgcg gtcgcccagt ctccccagga
     5161 ggctgaaagc accgtcgagg agatcgtggt gaccggcggc ttcgccaatt cgctggcccg
     5221 ggctctggcc gacaagcgca aggccgccaa cgtcatcgac gccatctcgg ccgaggatat
     5281 cggcaagttc ccgtccaaca acatcgccga ggccctgcag cgggtgccgg gcgtcgccat
     5341 cacccgcgac cggggcgaag gcctgttcgt gcgcgttcgc ggcctgggcc ccaacttcca
     5401 gatcgtgacc ctgaacggcc gcagcgccgc cgtgaacgaa aacatgcgcg acagcggcca
     5461 gagcggccgt cagttccgct tcgacaccct gccgtccgag ctggtggccg gtgtcgaggt
     5521 gggcaagacg ccgctggcct cgctggacga aggcgccatc ggcggcatcg tcaacgtcaa
     5581 gaccttccgc ccactggacc tgggcaagac caccgtcgcc gcctcggtca cagccagccg
     5641 gccggagctg gccgaccaga ccgatccgcg cgtctcgggc ctgatcaact gggtcaatga
     5701 agacaagagc ttcggcgccc ttctggctct ggtctatgac cagcgcaccc tgcgccagga
     5761 ccgcatcacc ggcgtgggct ggagctatgt caacacctcc accaccctgg gcaagagcct
     5821 ggccgctcag gggatcaccc tctatcccac tgccattcgc ccgacgctgg agcgcgagga
     5881 tcgcgagcgc aagggcctgg tgttctcggc gcaatatcgc ccgaccgacg ccacccagct
     5941 gacctttgac gccagctaca ccaagctcga cgaccactat gacgagttga cctattcggc
     6001 ggaccagggc gtcgcgacac tggttcccgg ctcggcggtc gtcaaggacg gcgtcctgac
     6061 cggtggcacg gtgacctcga ccagccagat cggccgcgag gtcagcgacc tggcgcatga
     6121 caacctgatg ctggcgctca acatccgcca gacggcgggc gagtggaccc tgtcgggtga
     6181 cctggcctat ggccgcgcct tctcggagac ctccaagccc atcacccgca cccgcctgct
     6241 ggggccgatc ggccagacga ccttctcgat gaccaaggct gatgacgagg ccgtgccaag
     6301 cgtcaagctg ctnnnnnnnn nngctgccgt tccgccgcat cgagtttcgg accaacgact
     6361 cgacggacga ggaaatcgcc ctgcaattcg acgccgagcg gccggtgtcg ctggggccgg
     6421 tgtcgcggat cgccttcggc gtgaagtatc gcgagcggga gcgcgactac aatcgcgccg
     6481 atatcaacta caccaccaac ctgaccggca agttcttcgg cgcgacctat ttcgaaccct
     6541 tccccgtcag cgacttcctt ggcgacgtgg acggaaccct gcccaccacc tgggccatgc
     6601 ccaatcccga cgccttctgg gtgctggccg acaagacgca gttgggcagg gctcgcgccg
     6661 atcagcgcaa ttcctactat gtctcggagg acatcgccgc cggctacgcc ctggccgata
     6721 tcgacacgag cgtgatgaac ctgcccttgc gcggcgaggt cggcgtgcgc gccgcccaga
     6781 ccaagcaggt ctcggccggc cacgccgaca atggtcgggc ggcgattccc gtccgctatg
     6841 tgaagaccta tagcgacgtt ctgccgacgg ccaatttcgt gctggacctt tccgagaccc
     6901 tgcaggcgcg tttcgcggcc gccaaggtga tcacccggcc ttccctggct gatctggccc
     6961 cgcgtctgac cctgaactcg tcgggcacgg tgctgaccgc cgtgggcggc aatccgcaat
     7021 tgcagccttt cgaggcctgg cagtatgacg ccaccctgga atggtacttc gccccgggca
     7081 gcgccctgat cgccggcgcc ttctacaagg acatcaccac ctttgtttac aaccagaaga
     7141 ccaacatcgt ggtcgacgga cagacctatc agctgacctc gcccaccaat ggcggtcagg
     7201 ccacgatcaa gggcgtggag ctggcctatc agcacctgtt caagagcctg ccggccccgt
     7261 tcgacggcct gggcttcctg gccaactata cctacaccga caccgaggcg acctattcgc
     7321 cgacgctgaa ggacgagatg gtcaatgtcg ccaagaacag cttcaacctg acgggcttct
     7381 acgagaagga ccgcttcgcg gcgcgcgcca gctacagctg ggtcgatgac gtgctgcagg
     7441 acgttggcgg ggccggcctc tcggcgctga atgacagcgc ctttggctca ctggacgcca
     7501 gcgtcagcta caagatcacg ccgcagatca cggcggcgct cgaagcccag aacctgacca
     7561 acgcggccca gtggcagttc gtcaagggcg gctggtttgg tggctacacc cactatggcc
     7621 gcaccgtgag ccttggtctg cgcgccaagt tctaaccctg ttctttgcga tcccctcaac
     7681 tggcccgcgc gcgcgtcgcg cgggcctttt ttgtgggaaa gaccagtaat gggcggcgcc
     7741 agacgccgct tcgtgaccgg gccttccgcg acctggctgc acccggcgcg gaggcttatc
     7801 gcgccggagc gccgttcggg gcgagcccct tgggcttcag catgccgtgc atgccgatcc
     7861 accgcacata ggcgtcgaac cagcccgtgc tcgtggtggt cttctgatac atcccaaagc
     7921 catggccgcc ctgctcatac aggtggaatt ccacggggcg tttggccgcc ctccagctgt
     7981 cgatcagccc aaagccgcta ttggcgaaga acggatcgtc ggcggccagc gccacgaaca
     8041 tcggcggcgc ctcggcgggg acggtcaccg ccgtcagcgg accatagacc aggccgatga
     8101 aggcgggctt ggcgtcctct ccggccaggg tggtcgccag ggtcagcatg gccccggccg
     8161 agaagccgat catgccgacg cggtccggat cgacccgcca ctcagccgcg cgctgacgca
     8221 ccagcgcgaa ggccgcccgg gagtcggcaa tctgaggggc caggccggcc atggccgcct
     8281 cggggctggg gcgcggcggc ctggcggtgg ccgagaacat ctcggtcatc gagcgctcga
     8341 accctggcat gtcggccggc gtctggttca gcctgtattt cagcacgaag gcggccaccc
     8401 ccttgtcggc gagcgcgcgg gcgacgtccc agccctcgtt ttccatcgac agcgtgcgga
     8461 acccgccgcc gggcgcgacg atcaccgccg cgccggtggc cttggccgga tcggggagga
     8521 agggggtcag ggtggcgacc gtgacattgc gcgaaaagag gctgttgtac tgctgatgcc
     8581 aggactccga cgccgtcgcg cccggcaagg ggcccgtgcc cnnnnnnnnn ngggggaaga
     8641 tctggcggcc agatttgtca aagcctgcgt gtttcatggc gcgtgttcct ggggttggtg
     8701 ggctgcgccc gacgtacggg ctgacgaccg ggatcggctc ctcggacggt tctggcctta
     8761 tttcgtcgtg gtcagccgga agaccatggt gttgcgatag gtctggccgg ggtccagacg
     8821 cgtcgacgcg aactgcgact tgttcggcgc atccgggaaa tgctgcggct ccagagcgat
     8881 gccgtcaccc tggcgataga tcttgccgct cttgccgacc gtggtcccgt cgatgaagtt
     8941 gccggcgtag acctgcaggc ccggctggtc gctgagaacc tccagcaccc ggccgctctt
     9001 gggatcggaa agacgcgcgg ccagacgcag ggcgccgccg gacttgccgt tcagcacgta
     9061 gttgtggtcg tagcctcgac ccatgaccat ctggggatcg ctggcgtcgc ggatcctggc
     9121 tctcacagcc gtgggctcgc ggaagtcgaa ggcagtacca gccacgggtg tggtctcgcc
     9181 ggtcgggatc agctcggcgt cgacgggcga gtagccatcg gccggaatgg tcagaacatt
     9241 gcccatcgcc ccgtcggccg agccttcgcc cgccatgttg aacagggcgt gattggtcat
     9301 gttgacgatc gtcggcttgt cggtggtggc gccgtaggtg accgtcagat tgttctgctc
     9361 gtccagggcg taggtggccg tggcgttcaa ggcgccgggg tagccctcct cgccatcggg
     9421 gctgagatag ccgaaggtga ccgaagccgt cgggccagac ttggcgtcca gcaccgtcca
     9481 gacgaccttg tcgaagccct tcacgccgcc gtgcagcgcc gcgacgccat tgttgttcag
     9541 cgccagctgg taggtcttgc cgtcgagcgt gaagcggccc ttgccgatcc ggttggcgaa
     9601 gcggccgacc gaagcgccga aatagctggc gttcctggcg taggcggccg cgtcggcgaa
     9661 gcccagcgcg atatcggccg tcttgcccgt gcggtccggt gcgatcaggg cctgcagggt
     9721 tgcgccatag gtgatgattg tggcgctgac gccctggcca ttggtcaggg tg
//
LOCUS       DAOMEO010000005         9412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1207_length_9412_cov_2.520145, whole genome shotgun sequence.
ACCESSION   DAOMEO010000005 DAOMEO010000000
VERSION     DAOMEO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1207_length_9412_cov_2.520145"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..467
                     /locus_tag="PLH31_00240"
     CDS             165..467
                     /locus_tag="PLH31_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87717.1"
                     /translation="MNKPVTNAPVSVSLPSSAVEDLSRRVGAGEFATLDEAVTAALLE
                     LEHFRAVELVGGEAAFTALAESVEVEAGLGEVDAFEFLHDLKAEYRRQAETRESQG"
     gene            464..751
                     /locus_tag="PLH31_00245"
     CDS             464..751
                     /locus_tag="PLH31_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR87718.1"
                     /translation="MKPVRLSKTATADIRSIGNDIAIDKPRAAEAFVEQIIQRCHSIA
                     DAPEGYGLRSAFGTGVRGVAIRPYVILYRVREKDILILGVRHGARRPTPFR"
     gene            complement(758..1954)
                     /locus_tag="PLH31_00250"
     CDS             complement(758..1954)
                     /locus_tag="PLH31_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQR87719.1"
                     /translation="MHRDTPAAPARNDLPARNDLIDTLRAFALIGVFMVHMFEQFEIY
                     WTAPQQTLTHILITTLMMGKAFALLALSFGLSFYILMDRATRRGEDFSRRFVWRMLLL
                     AVIGIVHGLIYRGDILIVLALLGIPLIPFYRASTGVLAGAAALFLLQPWMWLQIAIAA
                     SGAPWANTPPNHWTDPALAIYQTGDLVAVLKTNLFEAQVTKWWFFIETGRISLVMGLF
                     LIGLLLGRSGFFDAPQRFVGVRRIALAVLAITALALYLAKPHLTALLPAEPKPGMARV
                     MAGNLLGGWFDMALMGTYVLLIVEAYLGLAGKALGWLAPMGRMTLTFYVGQSLIWVPV
                     FYGFGLDAWKTLPQGTALWLGVGFMMLQAVIAQLWFRRFAYGPLEWLWRVATYRTWTV
                     PFVRRA"
     gene            2096..2398
                     /locus_tag="PLH31_00255"
     CDS             2096..2398
                     /locus_tag="PLH31_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87720.1"
                     /translation="MMKPIQPKPVTVRLSAEDAADLQARVDRGEFASLDEGVAAELAE
                     LNYRRAAEIVGSVEELEALLDELDFDLIVPAEPVAGNISLSQMLANLKTQAKAADE"
     gene            2401..3510
                     /locus_tag="PLH31_00260"
     CDS             2401..3510
                     /locus_tag="PLH31_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR87721.1"
                     /translation="MTFDFDAVVVGAGAVGLACGYALSKRGLVVAVLEEQGHIGQGVS
                     SRNSEVIHGGLYYASGSLKARLCVQGRRALYTFLDRHNVAYKKCGKLVVATSDEEVSR
                     LDAIWTQAADNDVEGMARLAGEQARALEPGLNAHAALLSPESGVFDSHGYMLALQGEI
                     EAAGGAVVIATPFEGADALPGGGFSVRAGGAEPTTLTCRLLVTAPGLSAQAVAARIDG
                     YPADRIPEGHFGKGIYFRLTGKAPFQRLIYPPPIPGALGTHYRNDLGGQAVFGPDLEY
                     VPSPDYSVDPAKAQVFAAYIRKFWPGLPDGALTPDYAGVRPKLHGAGQPQPDFQLRGA
                     DDHGLEGLIALFGIESPGLTSSLALGEEVAARLLA"
     gene            3660..4709
                     /locus_tag="PLH31_00265"
     CDS             3660..4709
                     /locus_tag="PLH31_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR87722.1"
                     /translation="MTAILHAMLGKGLGGLERVFLDYQPILEAYAAKHGGTCTGVVRR
                     GGSVSGAEAMRSPPLAVMPAFTDWDPWTVGAARRLVETVRPDLILSHGQRPARLFARL
                     APAGVVRAVCVHKPSFDVVPGTHYLCVGRHLAELAVARGVPSALAHFVPNAVAEPAVQ
                     ADPFAAPGTAIRIVAAGRLHPKKGFDVLIRAIGLLRARGLDVVCDIAGEGEERAHLEH
                     LIAELDLGAAVSLIGWTADISTFLAMGDLFAFPSHQEGFPLALLEAMAVALPVVSSAI
                     DGPVEMVADGETGRLVSPDDPAALADALADLIARPDVARTLGERARETVLRDYGSARL
                     ARRLEAVLDRMLARG"
     gene            4753..4829
                     /locus_tag="PLH31_00270"
     tRNA            4753..4829
                     /locus_tag="PLH31_00270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4787..4789,aa:Met,seq:cat)
     gene            complement(4850..5308)
                     /locus_tag="PLH31_00275"
     CDS             complement(4850..5308)
                     /locus_tag="PLH31_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR87723.1"
                     /translation="MDAKDRQIIRELQKDGRLTNQDLADRVNLSPSPCLRRLRNLEAE
                     GVITGYTALVDQKAYGLPITVFIRVRLDRHGQDVVKGFEDQVARIDQILDCFLLAGGD
                     DYLLRVIVESLEAYEDFIRRKLHAIPGIASIDTSFAYGVVKQTRVFPVAG"
     gene            5385..6596
                     /locus_tag="PLH31_00280"
     CDS             5385..6596
                     /locus_tag="PLH31_00280"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HQR87724.1"
                     /translation="MTHDLGEAGFSTRAIHAGYDPADEHGALTPPVHLTSTFAFDSAE
                     AGGEMFAGTRPGHFYSRISNPTTDLLERRVASLEGAEAGVATASGMGAITAVMWSFLR
                     SGDEVVTDKTLYGCTFAFMRDGLSRFGITVTQVDMTDLDALAAAVSDKTRIIYFETPA
                     NPNMRLVDIAGAAKVARACGAKLIVDNTYATPLLTRPIELGADIVVHSATKYLGGHGD
                     LIGGIAVGGVEDMARVRLTGVKDMTGAVMSPFTAFLVMRGLKTLALRMRQHCASAARV
                     AAWLEAHPAVTRVFYPGLKSFAQHDLAQRQMALPGGMMAFELAGGHDAGVAMMNRLAL
                     IRRAVSLGDAETLIQHPASMTHSTYSAEERALAGIGEGLVRLSIGLEDEADILADLAG
                     ALETAPSPVLA"
     gene            6767..7120
                     /locus_tag="PLH31_00285"
     CDS             6767..7120
                     /locus_tag="PLH31_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQR87725.1"
                     /translation="MSKYASGGEGDRRGAPRLATQRAGKLLCGAFAWDCTIRDVSDGG
                     VRVQMLAGVTPPAKVQLVDLTAGLAHDVQLAWQKDREVGLRILKTHDLRGLTHASART
                     AKNIWAASQARASAS"
     gene            complement(7196..7633)
                     /locus_tag="PLH31_00290"
     CDS             complement(7196..7633)
                     /locus_tag="PLH31_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR87726.1"
                     /translation="MSEETEGRRPNPVDLHVGGRVRMRRKLLGVSQEQLADSLGLTFQ
                     QVQKYERGANRVSASKLYEIAKTLQVPVSFFFDGLADPMGGSDVDDVGLHAERVVQEF
                     LTTPEGLELAEVFPRIARGRVRRQVLDLVRAMADDAGRSDTAA"
     gene            complement(7775..>7984)
                     /locus_tag="PLH31_00295"
     CDS             complement(7775..>7984)
                     /locus_tag="PLH31_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-L-homoserine lactone synthetase"
                     /protein_id="HQR87727.1"
                     /translation="VDALDMFAFGDLGAVQRQVKRAMTPQFSAGAQRDETLAAETLFR
                     LYDRSSQARRIWAEREGALPRVLHA"
     assembly_gap    7985..7994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7995..8601)
                     /locus_tag="PLH31_00300"
     CDS             complement(<7995..8601)
                     /locus_tag="PLH31_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HQR87728.1"
                     /translation="MIHVVTSENRHLYGPQLWAMHEQRRLQCVEKNGWVDLVVLDGGE
                     IDDYDDSRAIYLLGFDDAMALEVGLRLRPTDDRCMLADKFPHLIAPDESPKKGPDIWE
                     ASRLFTTEAYRRRKGAGRGERVFEAWAAAMELGLAHGVSRFVGMIDMQLYPGIMNSPI
                     DTRLVGLPRPYAYGVVAGSEIMLSRALLNRVLEALGREGSIG"
     gene            8815..>9412
                     /locus_tag="PLH31_00305"
     CDS             8815..>9412
                     /locus_tag="PLH31_00305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR87729.1"
                     /translation="MSPQTPIVDHPRVRSWLEIREPLERQLEPLGHAALKALRLSSGE
                     RVLDIGCGIGGTPAALAAAVGTRGDIVGLELLQAAVQVAERDPSLPENVSFVCGDAQD
                     YPFETGSFDAVFSRFGVMFFADPTTAFRNVKRALRPTGRLGFVCWRRFEENELDHLPL
                     RAAAPHLPPKLVSETTSSGWFSFSDSGCVRAILGNAGFV"
BASE COUNT         1548 a         3062 c         3194 g         1598 t
ORIGIN      
        1 cgctggccct gaagatcgcc gagatcccac aggccatccg cggctatggc cacgtcaaag
       61 aggcgtcggt ggttacggcc aaggcggccg aggccaagct gtgggagcag tgggcggggt
      121 gagggcctct ggctctcggg aaggttcgtg ataggcttca cgccatgaac aagccggtca
      181 cgaacgcgcc tgtttccgtc agccttccgt ccagcgcggt ggaggatctg agccgccgcg
      241 tcggcgccgg tgagttcgcg acgctggacg aggccgtcac ggcggccttg ctcgagctgg
      301 aacattttcg ggcggtcgag ctggtgggcg gtgaggcggc gtttaccgcc ttggccgaaa
      361 gcgtggaggt cgaggcgggt cttggcgaag ttgacgcctt cgaattcctc cacgacctga
      421 aggcggagta tcggcggcag gcggagaccc gggaaagcca gggttgaagc cggtccggct
      481 cagcaagacg gctacggccg atatccgcag catcggcaat gacatcgcca tcgacaagcc
      541 ccgcgccgcc gaggccttcg tcgaacagat catccagcgc tgccacagca tagccgacgc
      601 gccggaggga tatgggctgc ggtcagcctt tggaaccggc gtgcgcggcg tggcgatcag
      661 gccgtatgtc attctgtatc gcgtacgcga gaaggacatc ctgatcctcg gcgtgcggca
      721 tggcgccaga cggcccacgc ctttcaggtg atatcgatca agcccgcctc acgaacggca
      781 ctgtccacgt ccgatacgta gccacccgcc acagccactc cagcgggcca taggcgaagc
      841 gcctgaacca cagctgcgcg atcaccgcct gcagcatcat gaagcctacg ccgagccaaa
      901 gcgcggtccc ctgcggcagg gtcttccagg cgtccaggcc aaagccgtag aataccggaa
      961 cccagatcag cgactggccg acatagaagg tcagggtcat gcgacccatc ggggccagcc
     1021 agcccagggc cttgccggcc aggcccagat aggcctcgac gatcagcagc acataggtcc
     1081 ccatcagggc catgtcgaac cagccgccca gcaggtttcc ggccatcacc cgggccatgc
     1141 cgggcttggg ctcggcgggc agcagggcgg tcaggtgcgg cttggccaga tagagcgcca
     1201 gggcggtgat cgccagcacg gccagggcga tgcgtcgcac gccgacaaag cgctgtggcg
     1261 cgtcaaagaa tccgctccgc cccagcagca gaccgatcag gaacaggccc atcaccaggc
     1321 tgatgcgccc ggtttcgatg aagaaccacc acttggtcac ctgggcctcg aaaaggttgg
     1381 tcttcagcac cgcgaccaga tctccggtct ggtagatcgc cagggccgga tcggtccaat
     1441 ggttgggcgg cgtattggcc cagggcgcgc cagaggcggc gatggcgatc tgcagccaca
     1501 tccagggctg gagcaggaac agggccgccg cgccggccag cacccccgtc gaggcgcgat
     1561 agaacgggat cagcgggatg cccagcaggg ccagcacgat caggatgtcg ccgcgataga
     1621 tcagtccgtg gacaatcccg atcaccgcca gcagcagcat ccgccagacg aaccgccggg
     1681 agaagtcctc gccccgccgc gtggcgcggt ccatcaggat gtagaagctg agcccgaacg
     1741 acagggccag caaggcgaag gccttgccca tcatcagggt ggtgatcagg atgtgggtca
     1801 gggtctgctg gggcgcggtc cagtagatct cgaactgctc gaacatgtgg accatgaaca
     1861 cgccgatcag cgcaaaggcc cgcagcgtgt cgatcaaatc gttgcgtgcg ggcaggtcgt
     1921 tgcgcgcggg cgcagccggc gtatcgcggt gcatggtcga gcatcccccc ggaggcgccg
     1981 cttgattggt caggcgcggc gctgtgttgg tctgaccttt ggggctcggg cgcggaggtg
     2041 tcaaccgcac cgccgaggcg tggcatggcg gggcgttcat gataggctgg ccgacatgat
     2101 gaagccgatc cagcccaagc ccgtcacggt tcggctttcc gccgaggacg ccgccgatct
     2161 gcaggcgcgg gtcgatcgcg gcgagttcgc gtcgctggac gagggcgtcg ccgccgaact
     2221 ggccgagctc aactatcgcc gcgccgccga gatcgtgggt agcgtcgaag agcttgaagc
     2281 gttgctcgac gaactggact tcgacctgat cgttccggcc gagccggtcg ccggcaatat
     2341 ctcgctgtca cagatgctgg ccaatctgaa gacccaggcc aaggccgccg acgagtgagc
     2401 gtgaccttcg atttcgacgc cgtggtcgtc ggcgctggcg ccgtggggct ggcttgcggc
     2461 tatgcgctat ccaagcgcgg cctggtcgta gcggtgctgg aagagcaggg acacatcggg
     2521 cagggcgtgt cgtcgcgcaa ttccgaggtg atccacggcg ggctctatta cgcgtccgga
     2581 tcgctgaagg cgcggctgtg cgtgcagggg cgccgggcgc tctacacttt cctggaccgg
     2641 cacaatgtcg cctacaagaa gtgcggcaag ctggtggtcg ccaccagcga cgaggaggtt
     2701 tcgcgcctcg acgccatctg gacccaggcc gccgacaatg acgttgaggg catggcgcgg
     2761 ttggcgggtg agcaggcccg cgcgcttgag cccggcctca acgcccatgc cgccctgctg
     2821 tcgccggaaa gcggggtgtt cgacagccac ggctacatgc tggccctgca gggcgagatc
     2881 gaggccgccg ggggcgcggt ggtgatcgcc acgccgttcg agggcgcgga cgctcttcca
     2941 ggcggcggct tctcggtgcg ggccggcgga gccgagccca ccaccctgac ctgccgcctg
     3001 ctggtcaccg cgccggggct ttcggcccag gcggtggcgg cccggatcga cggctatccg
     3061 gcggaccgca ttcctgaagg ccatttcggc aaggggatct atttccgcct gaccggcaag
     3121 gcgcccttcc agcggctgat ctatccgccg cccatccccg gcgccctggg cacgcactat
     3181 cgtaacgatc tgggcggcca ggcggtgttt gggcccgatc tggaatatgt tccgtctccg
     3241 gactattcgg tcgacccggc gaaggcgcag gtgttcgccg cctatatccg caagttctgg
     3301 cccggcctgc cagacggcgc cctgaccccc gactatgccg gtgtgcggcc caagctgcat
     3361 ggcgcaggcc agccgcagcc cgacttccag ctacgcggcg ccgatgatca tggcctggag
     3421 gggctgatcg ccctgttcgg catcgaaagc ccgggcctga ccagctcgct ggccctcggc
     3481 gaagaggtcg cggcgcgact gctggcctag ctttttccaa tgggcgcgga gccagattga
     3541 agctgggcag ttttggtctg gcgccgcgcc gtccggcgga cgctacaaga gaacgccacg
     3601 ttgacacgac cccctcgtcc gcagatggtc cgcgcgcctc atcgcatcgg agcccgacct
     3661 tgaccgccat ccttcacgcc atgctgggca aggggttggg cgggctcgag cgcgtcttcc
     3721 tcgactatca gccgatcctg gaggcctatg ccgccaagca cggcgggacc tgcaccgggg
     3781 tagtgcgccg gggcggatcg gttagcggcg cggaagcgat gcgctcgccg ccgctggcgg
     3841 tgatgcccgc cttcaccgat tgggatccct ggacggtggg cgcggcgcgc cggctggtcg
     3901 agacggtgcg cccggacctg atcctcagcc atggccagcg gccggcgcgc ctcttcgcaa
     3961 ggctggcccc agccggcgtg gtgcgggccg tctgtgtgca caagccatcc ttcgacgtgg
     4021 ttccgggaac ccattatctc tgcgtgggac gccacctggc cgagctggcg gtggcgcggg
     4081 gcgtaccttc ggctctggcc cacttcgtgc ccaatgcggt ggccgagccg gcggtccagg
     4141 ctgatccgtt cgccgcgcca ggcacggcga tccgcatcgt cgcggccggc cgcctgcatc
     4201 ccaagaaggg ctttgacgtc ctgatccgcg ccatcggcct gctgcgcgcg cgcggcctgg
     4261 acgtggtctg cgacatcgcc ggcgaaggtg aggagcgggc gcatctggag cacctgatcg
     4321 ccgagctgga cctgggcgcc gctgtcagcc tgatcggctg gaccgccgat atctcgacct
     4381 tcctcgcgat gggcgatctc ttcgccttcc cctcgcatca ggagggcttc cccctggcgt
     4441 tactggaagc catggccgtg gctctgcccg tcgtctcctc ggccattgat ggcccggtcg
     4501 agatggttgc ggacggcgaa accggccgtc tggtttcgcc agatgatccc gccgccttgg
     4561 ccgacgcgct tgccgatctg atcgccaggc ctgacgtggc gcgaaccctg ggggaacgcg
     4621 cccgcgagac cgtcctgcgc gattacggct ctgcgcgcct ggcgcgacgt cttgaagcgg
     4681 tccttgaccg gatgcttgcc cggggttgat gccccggccg cttgggcgta tgaccccgct
     4741 ccgtcctggg acggttgggt agctcagatg gttagagcgg tggattcata acccacaggt
     4801 cggcggttcg atcccgcctc caaccaccag gacggtcttc cagcaccctt caccccgcca
     4861 ccggaaacac ccgcgtctgc ttgaccacgc cataggcgaa gctggtgtcg atcgaggcga
     4921 tgccggggat ggcgtgcagc ttgcggcgga tgaaatcctc ataggcctcc aggctctcga
     4981 cgatcacccg gagcagatag tcgtcgccgc cggccagcag gaagcagtcg aggatctggt
     5041 cgatccgcgc cacctggtcc tcgaagccct tgaccacgtc ctggccgtga cggtccaggc
     5101 gcacgcggat gaagacggtg atcggcagac cgtaagcctt ctgatcgacc agggccgtgt
     5161 agccggtgat caccccctcg gcctccagat tgcgcagtcg gcgcaggcag ggggacggcg
     5221 acaggttcac ccggtcggcc aggtcctgat tggtcaggcg cccatccttc tgcagttcgc
     5281 ggatgatctg ccggtccttg gcgtccattg ggcgctcctt gagtggattc tgccaatcta
     5341 gtcaccagat ctggcagact tggcaatctt ccatcgcctg atccatgacg cacgatcttg
     5401 gcgaagcggg cttttcgacg cgggcgatcc acgccggcta tgatccggcc gacgagcacg
     5461 gcgccctgac gccccccgtc cacctgacct cgaccttcgc cttcgacagc gccgaggccg
     5521 gcggcgagat gtttgccggc acgcgtccgg ggcatttcta ttcccggatt tccaacccca
     5581 ccaccgacct gctggagcgc cgcgtggcct cgctggaggg cgccgaggcc ggcgtcgcca
     5641 cggcctcggg catgggcgcg atcacggcgg tgatgtggag cttcctgcga tcgggcgacg
     5701 aggtggttac tgacaagacc ctgtatggct gcaccttcgc cttcatgcgc gacgggctct
     5761 cgcggttcgg gatcaccgtc acccaggtcg acatgaccga tctggacgcc ctggcggcgg
     5821 cggtgtctga caagacccgg atcatctatt tcgagacccc ggccaatccg aacatgcggc
     5881 tggtcgatat cgccggcgcg gccaaggtgg cgcgcgcctg cggggccaag ctgatcgtcg
     5941 acaacaccta cgccacgccc ctgctgacgc ggccgatcga gcttggggcc gatatcgtcg
     6001 tgcactcggc caccaagtat ctgggcggtc atggcgacct gatcggcggc atcgcggtcg
     6061 gcggtgtcga ggacatggcc cgggtgcggc tgaccggggt caaggacatg accggggcgg
     6121 tgatgtcgcc gttcacggcc tttctggtga tgcgcggcct gaagaccctg gccctgcgca
     6181 tgcgccagca ctgcgccagc gccgccaggg ttgcggcctg gctggaggcc catcccgccg
     6241 tgacgcgggt gttctatccg gggctgaaga gctttgccca gcacgacctg gcccagcgcc
     6301 agatggcttt gccgggcggg atgatggcgt ttgagctggc cggtgggcac gacgccgggg
     6361 tggcgatgat gaaccgcctg gccctgatcc gccgggccgt gtcgctgggc gacgccgaga
     6421 ccctgatcca gcacccggcc agcatgaccc actcgaccta tagcgccgaa gagcgcgccc
     6481 tcgccggcat cggggaaggg ctggtgcggc tgtcaatcgg gctggaggat gaggccgata
     6541 tcctggctga tctggccggc gcgctcgaga cggcgccttc gccggtcctc gcctgacggc
     6601 cccaaaaggt taatgccgcc ggcgccatga tccagggcgt cgacgggggt cgactatccg
     6661 acaaaaccga ctggtttcag aggcttgtgc gagacgtatg gtcgcgggcg cgacaaaagc
     6721 gccgcgtctg cacggccgat taaccgcgcc atgcgtttat gcaatcatgt cgaagtacgc
     6781 gtcaggcgga gaaggtgatc ggcggggcgc gccccgtctg gcgacccaaa gggcaggaaa
     6841 actgctctgc ggcgccttcg cctgggattg cacaatccgc gacgtctctg atgggggcgt
     6901 tcgggtccag atgctggcag gcgtcacgcc gcccgcaaag gttcagcttg tcgatctgac
     6961 ggccggcctg gctcacgatg tccagctggc ctggcagaag gaccgcgagg tgggcctgag
     7021 aatcctcaag acccacgatc tgcgcggctt aacccacgct tcggcgcgga cggccaagaa
     7081 tatctgggcg gcgtcccagg cgcgggcctc ggccagctag cctggcgctt gagatacaaa
     7141 aagaccccga cagccacccg tcggggtccc cttgatcata cgtccagcaa tgggctcagg
     7201 cggcggtgtc gctgcgaccc gcatcgtcgg ccatggcccg gaccaggtcg agaacctgac
     7261 gacgcacacg accgcgcgcg atgcgcggga acacttcggc cagctccagg ccctccggcg
     7321 tggtcaggaa ctcctgcacc acgcgctcgg cgtgcaggcc gacgtcatca acgtcagagc
     7381 cgcccatcgg gtcagccaga ccgtcaaaga agaacgacac gggcacctgc agcgtcttgg
     7441 cgatctcgta gagttttgag gcgctgacgc gattggcgcc gcgctcgtac ttctgcacct
     7501 gctgaaaggt caggcccagg gaatcggcca gttgctcctg gctgacgccc agcagtttgc
     7561 ggcgcatccg aacgcgcccg ccgacatgca gatcgaccgg attgggtcgc cggccctcgg
     7621 tttcttcgct catatttcgc ctccagtggt tgtttgtgca acatgacacg accgtgacgg
     7681 cggcgaaagt caggcgcggc gttaggtcgc gcaagttgcg attctgtccc caagatgggt
     7741 tttcactatc gctttcagag agttacgcgt ttggctatgc gtgcaaaacg cgaggtagcg
     7801 ctccctcgcg ctcggcccag attcggcggg cctggctgga ccggtcgtac aggcgaaaga
     7861 gggtctcggc ggccagggtt tcgtcgcgct gggcgcccgc cgaaaactgg ggcgtcatgg
     7921 cgcgcttcac ttgccgttgc acggcgccca gatcgccgaa cgcgaacatg tccagggcat
     7981 ccacnnnnnn nnnnagccga tcgacccctc gcggcccagg gcctcaagca cgcggttgag
     8041 cagggcgcgc gacagcatga tctctgaccc ggcgaccacg ccataggcat agggccgggg
     8101 caggccgacc agacgcgtat cgatgggtga gttcatgatg cccggataga gctgcatatc
     8161 gatcatgcca acgaagcgac tgacgccatg cgccaggccc agttccatcg ccgccgccca
     8221 ggcttcgaac acccgctcgc cacgcccggc gcccttgcga cggcgatagg cttccgtcgt
     8281 gaacaggcgg ctggcctccc agatgtctgg acccttcttg ggcgactcgt ccggggcgat
     8341 caggtgcggg aacttgtcgg ccagcatgca gcgatcatcg gtcgggcgca ggcgcagacc
     8401 gacctccagc gccatggcgt catcaaagcc gagcagatag atggcgcggc tgtcgtcata
     8461 gtcgtcgatc tcgccaccat ccaggacgac caggtccacc cagccattct tttcgacgca
     8521 ctggagccga cgctgctcat gcatcgccca cagttgcggg ccgtacagat gacgattttc
     8581 agacgtgacg acatggatca tgaccgtttc ccccaggaag tgacggtcgc gactaggtgt
     8641 caggttctgg cgcgctggcg ataggccaaa cggcctatgc cgggcgccgg gggctgtttg
     8701 cgaggggcag gaggtgttgt gtcttcgcgt cgggctcaga cggaggcaag gtctgcttgg
     8761 ggtgggttgc tgaaaccggc gcccgcctgg aattttgcgt ggtaaagctc acgagtgtcg
     8821 ccacaaactc ccattgtcga tcacccgcga gttcgaagct ggctggaaat ccgggagccg
     8881 ctggagcggc agctggaacc acttggacac gccgcgctga aggcgcttcg gctttcgtca
     8941 ggcgaacggg ttctcgacat aggctgtgga attggcggga cgccggctgc gctggcggcg
     9001 gccgtcggga cgcggggcga cattgtcggc ctggaactat tgcaggccgc tgtccaagtc
     9061 gctgaacgtg atcctagtct gcctgagaac gttagtttcg tgtgcggcga cgcacaggat
     9121 tatcccttcg agaccggatc attcgacgcg gtgttctcgc ggttcggcgt catgttcttt
     9181 gccgatccaa caactgcctt tcgcaacgtg aagagagcgc ttcggccaac cgggaggctc
     9241 gggttcgtct gttggcgaag gttcgaggag aacgaactgg accacctgcc gcttcgggcc
     9301 gccgcgccgc atctgccgcc gaagctggtg agcgagacca ctagttctgg ttggttctcg
     9361 ttttcagatt cgggctgtgt tcgcgcgata cttgggaacg ctgggttcgt cg
//
LOCUS       DAOMEO010000006         9247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1227_length_9247_cov_2.492711, whole genome shotgun sequence.
ACCESSION   DAOMEO010000006 DAOMEO010000000
VERSION     DAOMEO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_9247_cov_2.492711"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6902)
                     /locus_tag="PLH31_00310"
     CDS             complement(<1..6902)
                     /locus_tag="PLH31_00310"
                     /inference="COORDINATES: protein motif:HMM:NF040115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice nucleation protein"
                     /protein_id="HQR87730.1"
                     /translation="MSISSLSAAQISALPASAIQAQTTTEIQSLSSTQIGAINASGIE
                     VLADTQVAALSATQIKALTTTQIPKFAPNVVFDVSQLTTLSGQQVSALTSTQLAGLDT
                     SHIGALSATQVSVLSATKFSGLVDTQMAALSTTQIKGMTTTQLKGLTTTDIGELANTQ
                     VQALSVAQLGALSGAQVSSFDVTQVAALSTTQIGSLTPTQLKGFTTADIGELSTTQVG
                     ALSAAQIGALAATQVSAMADTQVAALSVTQVRGLTTVQLKDLTTADMGKLLDTQVGAL
                     TAVQVTALSATNVSALVDTQVAALTATQIKGLSVTQVKGLSAADIGELSETQVGAMTT
                     AQVAVLDVTKVQALSETQVSALSAAQVGALGATNFSALADTQVAAFNETQIKGLTASQ
                     LSALTTADVAELADTQVAALTPVQVSSLSATSVSTLNATQTAALSTTQIKALTPIQLK
                     GLTTADIAELADTQVAALTPAQLSSLSATNISALDQTQMAALSVTQVKGLTSVQIKGL
                     STGDVGELSTTQIGALTAEQLRAMSETNISALVDTQIAALSNSQVQALTTTQLKGLSV
                     ADIGELSSTQVSALSTAQIGALSVTNLQQLSTTQIGGISTSQIAALSATNVKLLADTQ
                     FAALSTTQIKGFTAGQLAALTTTNVSYLADTQVGALTPAQIGSLSATNVSALADTQMA
                     ALSATQIKGLTVNQLKGLSAADIGELADTQVGALTAAQVGALSATAVSALNETQTAAL
                     SATQIKGLTPTQLKGLSTADMGELVDTQIQALTVAQLGSLTATNISALNETQTQALTA
                     TQIKGLTTTQLKGLSTADIAELTADQIGAMTPSQTAALGVTNLQALTTTQIGAMSASQ
                     IGAMGATAFLALADTQIAVLNATQIKGLTTSQLSALTAGDILEFADTQVAALAATQIS
                     ALSATGVSALNEDQTAALTTTQIKALTATQLKGLSPADIGDLADTQIAALTPTQLSSL
                     SATGISALNETQTAALSATQIKGLTATQIKGLSTADIGELLDTQVAALTTEQLKALSE
                     TNISALSETQVSALTGTQVAALTATQLKGLSTADIDELSSTQIGQLGAAQISALSVTN
                     LQQLSPTQIGGLTATQIGAISATNLIELNSFQLAALSESQIKGLNVSQIKALGVDEIV
                     ALADTQIGALTATQISALTTTALNNMSGDHFAALSTTQVKALTTTQVAGFNSWAVGSL
                     LDTQVATLSATQVAALSTTSVNDILYNDTQVAALTTTQVKALTATQVGSITGGIQGLD
                     TTQLGALTATQMSSLTGNGVSAMTDTQAATLTTTQIGALKTAAFEALLGAHSDAIQTT
                     QIAGFTTSQIAGLPSGMAVGTLDETQFGAFSASQIGALKITDIQGLSDADIGVLSTTQ
                     IKGFTASQMAAMHPGVTDALVATQIEALTTSQVAALNATSFKYLDETQTAGFTTTQMK
                     ALTTTQLKMLDGGEYADFNVTQLGSLSAAQVGSLQASYLASLDEPQLQAFSATQVKGL
                     TATQLKGLSETDVSQLSTTQIGALSSEQLKAFADTAISALTQTQVGALSNSQVQSLTT
                     TQLKGLSTDDITELSATQVGALASAQVAALSITNLQQLSETQIGGLSATQISALAATS
                     ITQLADTQLAALSTTQIKGLTVAQLSKLTADNIADLADTQVAALSAAQLGALSATGFS
                     ALADTQVAALSTTQIKGVTATQLKALTTADIGELVDTQVAALSTVQLSALSATNISAL
                     NETQTAALSTTQIKGLTATQLKGLSTADMAELTTTQVAAMSAEQLKALSETNVSALSE
                     TQVGALSNAQVQALSTTQLKGLSVADIGELSATQVGALSAAQVGALSVTNLQALTETQ
                     IGGLTATQIGALSVTNVTQLADTQFAALSTTQIKGLTTAQVARLTADNVGDLADTQIG
                     ALSAAQIGALSATNVSALADTQVAALSATQIKGLTATQLKGLSAADIGELADTQIAAL
                     TAIQLGALSTTGVSALADTQMAALSATQIKGLTTTQLGGLSTADIGELLDTQVAALAA
                     SQLAALSATNISALNETQVAAISTTQIRALTATQLRGLSPADIGELSATQIGALTTSQ
                     ITALDTTNLQALSETQVGALSAAQISVLTSTGISALADTQIAVLNATQIKGFNASQLK
                     ALTTADIGELADTQIAALTPTQLTSLTATNISALDDTQVAALSTTQIKALTATQLKGL
                     NTADMAQFSETQIGALTAEQLKALSETNVSALVDTQVAALSNTQVQALTATQLKGLST
                     ADVGELLATQIGALTAAQIGAFTSTNVQQLSTTQVAALSATQIAGLTTTNVKA"
     gene            complement(7431..>9247)
                     /locus_tag="PLH31_00315"
     CDS             complement(7431..>9247)
                     /locus_tag="PLH31_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR87731.1"
                     /translation="PLAESGSFDPHLCERTARAAASEASASGLHWTFAPMVDVARDQR
                     WGRVAEGAGEDVYLGQVLAEARVRGFQGKDLKAEDSMLATPKHFAAYGAVTAGLDYNA
                     VELSEATLREVHLPPFQAAFAAGALTTMSAFNDINGVPSTGNKRLMTDILRGEWGFKG
                     VVISDYTADQELVAHGFAADDRDAARLAILAGVDISMQSGLYLRFLPELTASGEVPMA
                     VVDASVRRVLALKEALGLFDNPYRSINAKVEAMRTATPALKQLSREAGGRSIVLLRNE
                     GGILPLPRAGQRLALIGPFAEDRDNVVGPWAFFGDKSLNVDLATGLRAAMTDPASLIV
                     ERGCEVETTLAGGFERAVAAAQAADVVLLAIGESQDMTGEAKSRTDIRIPPVQQRLAE
                     AIAATGKPVVVLLRHGRAIALEGVVRDARAILATWFLGSEMGNAVADVLFGAVNPSAR
                     LPVSFPLESGQEPFFYNGRTTGRPAPVDPNAQEYKARWRSIRNEALYPFGFGLGYTTF
                     ALSDLKLSTPQLAWGDTLHIAARLTNTGAVQGEHVVQLYIRDRVASRTRPKFELKREH
                     LMFVGEGDRWVVESGLFDVWVANSSVDGLAGSFELLKA"
BASE COUNT         1362 a         2848 c         3349 g         1688 t
ORIGIN      
        1 gccttgacgt tggtcgtcgt cagacctgcg atctgggtcg cgctgagcgc cgcgacctgg
       61 gtggtcgaca gctgctggac gttggtgctg gtgaaggcgc cgatctgagc cgccgtcaga
      121 gcgccgatct gggtggccag cagctcgccg acgtcggcgg tggacaggcc cttcagctgg
      181 gtcgccgtca gcgcctggac ctgggtattg ctcagagccg cgacctgggt atcgaccagg
      241 gccgagacat tggtctcgga cagggcctta agctgttccg ccgtcagggc gccgatctgg
      301 gtctcggaga actgcgccat gtcggcggtg ttcagaccct tcagctgggt ggccgtaagg
      361 gccttgatct gggtggtcga cagggccgcg acctgggtgt cgtcgagcgc cgagatgttg
      421 gtcgccgtca ggctggtcag ttgcgtcggc gtcagggccg cgatctgggt gtcggccagc
      481 tcaccgatgt cggcagtcgt cagggccttc agctgcgaag cgttgaagcc cttgatctgc
      541 gtggcgttca ggaccgcgat ctgtgtatcg gccaaggccg agatgccggt cgaggtcaga
      601 acgctgatct gggcggcgct gagggcgccg acctgggttt ccgacagggc ctgcaggttg
      661 gtggtgtcca gggccgtgat ctgcgacgtg gtcagggcgc cgatctgggt ggccgacagc
      721 tcgccgatgt cggcgggcga caggcccctc agttgggtcg ccgtcagggc cctgatctgg
      781 gtcgtggaga tggccgcgac ctgggtctcg ttcagggccg agatgttggt ggcgcttagc
      841 gccgccagtt gcgaggcggc cagggcggcc acctgggtgt cgagcagctc gccgatatcc
      901 gccgtgctca gaccgcccag ctgggtggtc gtcaggccct tgatctgggt ggcgctcagg
      961 gcggccatct gggtgtcggc cagggccgag acgccggtgg tcgacagggc gcccagctgg
     1021 atggccgtca gggctgcgat ctgggtgtcg gccagctcgc cgatatcggc ggcgctcagc
     1081 cccttcagct gcgtcgccgt caggcccttg atctgggtgg cgcttagggc tgcaacctgg
     1141 gtatcggcca gagccgagac attggtcgcg ctgagggcgc cgatctgggc ggcgctgagg
     1201 gcgccgatct gggtgtcggc cagatcaccg acgttgtcgg cggtgaggcg ggcaacctga
     1261 gccgtggtca agcccttgat ctgggtggtg ctgagcgccg cgaactgggt atcagccaat
     1321 tgggtgacat tggtcaccga cagcgcgccg atctgggtag cggtgagccc gccgatctgg
     1381 gtctccgtca gcgcctgcag gtttgtgacc gacagggccc cgacctgggc ggcgctcagg
     1441 gcgccgacct gggtggccga cagctcgccg atatcggcga ccgacaggcc cttcagctga
     1501 gtggtgctca gcgcctggac ctgggcgttg ctcagggcgc cgacctgggt ctcactgaga
     1561 gccgagacat tggtctccga cagcgccttc agctgctcgg cgctcatggc ggccacctgg
     1621 gtagtcgtca gttcggccat gtcggcggtc gacagaccct tcagctgagt cgccgtcaga
     1681 cccttgatct gggtcgtcga cagggccgcc gtctgggttt cgttcagggc cgagatgttg
     1741 gtggcgctca gggcgctcag ctggacggtg gacagagccg ccacttgggt gtcgaccagt
     1801 tcgccgatat cggccgtcgt cagggccttc agctgcgtgg cggtcacgcc cttgatctgg
     1861 gtggtgctca gggccgcgac ctgggtgtcg gccagggccg agaagccggt cgcgctcagg
     1921 gcgcccagct gggcggcgct cagggcggcg acctgggtgt cggccaggtc agcgatgtta
     1981 tcagccgtca gcttcgacag ctgagccacc gtcagaccct tgatctgggt ggtgctcagc
     2041 gcggccagct gcgtgtcggc cagctgggtg atgctggtcg ccgccagggc gctgatctgg
     2101 gtggcgctca ggccgccgat ctgggtttca gacagctgct gcaggttagt gatcgacagg
     2161 gccgcgacct gggccgaggc cagggcgccg acctgggtgg ccgacagctc ggtgatgtcg
     2221 tccgtcgaca gacccttcag ctgggtcgtg gtcagggact ggacctggct attgctcagg
     2281 gcgccgacct gggtctgggt cagggccgag atggcggtgt cggcgaaagc cttcaactgc
     2341 tcgctgctca gagcgccgat ctgggtcgtg gacagctgac tgacgtctgt ttcggacagc
     2401 cccttcagct gggtcgccgt caggcccttg acctgggtcg ccgagaaggc ctggagctgg
     2461 ggctcgtcaa ggctggcgag gtaggacgcc tgcaggctgc ccacctgggc cgccgagagc
     2521 gagcccagct gcgtgacatt gaagtcggca tattcgccgc catctagcat cttcagctgg
     2581 gtcgtcgtca gggccttcat ttgggtggtg gtgaagcccg cggtctgggt ctcgtccaga
     2641 tacttgaagc tggtcgcgtt gagcgcggcg acttggctgg tcgtcagggc ctcgatctgg
     2701 gtcgcgacca gggcgtcggt cacgcccggg tgcatcgccg ccatctgcga ggcggtgaag
     2761 cccttgatct gggtggtgct cagcacgccg atatcggcgt cgctcagacc ctggatgtcg
     2821 gtgatcttca gcgcgccgat ctggctggcg ctgaaggcgc cgaactgggt ctcgtccagc
     2881 gtgccgacgg ccatgccgct gggcaagccg gcgatctgcg aggtggtgaa gcctgcgatc
     2941 tgggtggtct ggatggcgtc agagtgcgcg ccaagcagcg cttcaaacgc tgccgtcttg
     3001 agggcgccga tctgggtcgt cgtgagcgtg gcggcctggg tgtcggtcat ggcgctgacc
     3061 ccgttgccgg tcagcgacga catctgcgtg gccgtcagcg cgcccagctg ggtggtgtcc
     3121 agcccctgga tgcccccggt gatcgaaccg acctgggtcg ccgtgagggc cttgacctgg
     3181 gtggtggtca gggctgcgac ctgggtgtcg ttatacagta tatcgttgac gctggtggtt
     3241 gacagcgcgg ccacctgagt ggccgacaac gtcgcgacct gggtgtcgag caggctgccc
     3301 acagcccagc tgttgaaccc tgcgacctgg gtcgtcgtca gagccttgac ctgggttgtc
     3361 gacagcgccg cgaaatggtc gccgctcata ttgttgagcg cggttgtcgt cagggcgctg
     3421 atctgggtgg ccgtcagggc cccgatctgc gtatccgcca gcgcgacgat ctcgtcgacg
     3481 cccagggcct tgatctgcga gacgttgagc cccttgatct ggctctcgga caacgcagcc
     3541 aactggaagc tgttgagctc gatgaggttc gtcgccgaga tggcgccgat ctgtgtggcg
     3601 gtgaggccgc cgatctgggt ggggctcagt tgctgcaggt tggtcaccga cagcgcgctg
     3661 atctgggctg cgcccagctg accgatctgg gtggacgaca gttcgtcgat atcggcggtc
     3721 gacaggccct tcagctgggt cgccgtcagc gccgcaacct gcgtgccggt cagcgccgaa
     3781 acctgggtct cgctcagggc cgagatgttg gtttccgaca gcgccttcag ctgttcggtg
     3841 gtcagggccg ccacctgggt gtcgagcagc tcgccgatgt cggcggtcga caggcccttg
     3901 atctgggtgg cggtcagacc cttgatctgc gtcgcgctca gcgcggccgt ctgggtctcg
     3961 ttcagggccg agatgcccgt ggcagacagg ctggacagct gggtcggggt cagggccgca
     4021 atctgggtgt cggcgaggtc gccgatgtca gcgggcgaca ggcccttaag ttgggtggct
     4081 gtcagggcct tgatctgggt cgtggttagc gcggcagtct ggtcttcatt gagcgcggag
     4141 actcccgtcg cgctcagcgc cgaaatctgg gtcgcggcca gggccgcaac ctgggtgtcg
     4201 gcaaattcaa ggatgtcgcc ggcggtcagg gccgacagtt gcgaggtcgt caggcccttg
     4261 atctgcgtgg cgttgagcac ggcaatctgg gtgtcggcca gggccaggaa ggccgtcgcg
     4321 cccatggcgc cgatctgcga agcgctcatg gcgccgatct gagtggtcgt cagggcctgc
     4381 aggttggtca cgcccagggc tgcagtctgg gatggcgtca tggcgccgat ctgatcggcc
     4441 gtcagctcgg cgatgtcggc ggtcgacaga cccttcagct gggtcgtcgt cagacccttg
     4501 atctgcgttg cggtcagagc ctgggtctgg gtctcgttca gggccgagat gttggtcgcc
     4561 gtcagcgagc ccagttgggc caccgtcagg gcctggatct gggtgtcgac cagctcgccc
     4621 atgtcggcgg tagacaggcc cttcagctga gtgggggtca ggcccttgat ttgtgtcgcg
     4681 ctcagcgccg cagtctgggt ttcattcagg gccgaaaccg ccgtcgccga aagggcgccg
     4741 acctgcgccg cggtcagggc gccaacctgg gtgtcagcca gctcgccgat gtcggcggcc
     4801 gagagaccct tcagctgatt gaccgtcagg cccttgatct gggtggcgct cagagccgcc
     4861 atctgggtgt cggcgagggc cgagacgttg gtcgcgctga gcgagccgat ctgggccggc
     4921 gtcagggcgc cgacctgggt atcggccaga tagctgacat tggtggtcgt caaggcggcc
     4981 agctggccgg ccgtgaagcc cttgatctgg gtggtgctca gcgccgcgaa ctgggtatcg
     5041 gccagcagtt tgacgttggt cgcggacagc gcggcgatct gcgaggtgct gatgccgccg
     5101 atctgggtgg tcgacagctg ctgcaggttg gtgaccgaca gcgcgccaat ctgggccgtg
     5161 ctcagggcgc tgacctgggt cgacgacagc tcgccgatgt cggcgaccga caggcccttc
     5221 agttgggtcg tggtcagggc ctggacctgg ctgttgctga gggcggcgat ctgggtgtcg
     5281 accagggccg agatattggt ctcggacatc gccctgagct gctcggccgt cagggcgccg
     5341 atctgggtgg tcgaaagctc cccgacgtca ccggtggaca gacccttgat ctgcacggac
     5401 gtcaggccct tgacctgcgt cacactgagc gcggccatct gggtctgatc cagcgccgag
     5461 atgttggtgg ccgacaggct ggaaagctgc gccggagtca gggcagccac ctgcgtgtcg
     5521 gccagttcgg cgatgtcggc ggtcgtcagt cccttcagct ggatcggcgt cagagccttg
     5581 atctgcgtcg tgctgagcgc ggcggtttgg gtggcgttca gggtggacac gctcgtggcg
     5641 ctgagcgacg aaacctggac cggcgtcagg gccgccacct gcgtgtcggc cagctcggcg
     5701 acatcggctg tggtcagggc cgaaagctgc gaggccgtca ggcccttgat ctgggtctcg
     5761 ttgaaagccg cgacctgggt gtcggccagg gccgagaagt tcgtggcgcc cagagcgccg
     5821 acctgcgcgg ccgaaagggc gctgacctgg gtttccgaaa gggcctgcac cttggtgacg
     5881 tccagcaccg cgacctgggc cgtcgtcatc gcgccaacct gggtctcgga gagctcgccg
     5941 atgtcggcgg cgctgaggcc cttaacctgg gtgaccgaga ggcccttgat ctgggtcgcc
     6001 gtcagggccg caacctgcgt atcgaccaga gccgacacat tggtggcgct gagcgccgtc
     6061 acctgcaccg ccgtcaaggc gccaacctgg gtgtcgagca gcttgcccat gtcggcggtc
     6121 gtcaagtctt tcagctgaac cgtcgtcagg cccctgacct gggtgacgct gagcgccgcc
     6181 acctgggtgt cggccatggc cgacacctgg gtcgccgcca gcgcgccgat ctgcgccgcc
     6241 gacaacgcgc cgacctgggt ggtcgaaagc tcgccgatgt cggcggtcgt gaagcccttc
     6301 agctgcgtcg gcgtgaggct gccgatctgg gtggtggaca gcgccgccac ctgggtgacg
     6361 tcgaaggacg agacctgggc gcccgacagc gcgcccagct gggcgaccga gagagcctgc
     6421 acctgggtat tggccagttc gccaatgtcg gtggtggtca gacccttcag ctgggtcgtc
     6481 gtcatgccct tgatctgggt ggtcgagagc gccgccatct gggtgtcgac gagacccgag
     6541 aacttggtgg ccgacagcac gctcacctgc gtcgcgctca gcgcgccgat atgggaggtg
     6601 tccaagccgg ccagctgagt ggaggtcagg gccgaaacct gctggccgct gagggtggta
     6661 agctgactga catcgaagac cacgtttggc gcgaacttcg ggatctgggt ggtggtgagg
     6721 gccttgatct gggtcgcgct gagcgccgcg acctgggtat cggccagaac ctcaatgccg
     6781 ctggcgttga tggccccgat ctgggtggag ctgagcgact ggatctcggt ggtcgtctgc
     6841 gcctggatgg cgctggcggg aagggccgag atttgagcgg cggagaggct cgagatcgac
     6901 attcagctgt cctggccggg ggccgcgcgg ggcgcgcgca ctctaacact ttgtgggatt
     6961 ggagcctggg tacgcgggcg ccgactcgaa cctgttacgg ttgagttccg gtaaccttat
     7021 tttctttcca agaggtttca acgggacatg ccgacgcagt ctccgttaac cgtgacttgt
     7081 ctggtcagaa gaacgaggtg atgaggccga gcccacacaa acgcctttaa tatcaaggct
     7141 tggctgtgca aatgcagggg ccttcgggtc ttgcgggcgg cccagacgca tgtgtcacgg
     7201 aagtgtagtt cttactgcca ggcgcccaag atgaagcaac ttccagacct ggagagcact
     7261 tggtgattcg cccgatctta atgaacggac caattcacac ctaaaatggc ggctgaaact
     7321 ccgcttggaa tgtcgccggc gatggcgggc tcgcgcgatg ctctcactgc ggctggcctg
     7381 aaggctccga tggaaacgac ggctccgcga ctggcagtgt tcgagcggat ctaggccttc
     7441 agcagctcga aactacccgc cagcccgtcg accgacgagt tggccaccca gacatcgaac
     7501 aagcccgact ccacgaccca gcgatccccc tcgccgacga acatcaggtg ctcgcgcttc
     7561 agctcgaatt tcggccgggt gcggctggcg acgcggtcgc ggatatagag ctgcaccacg
     7621 tgctcgccct gcacggcgcc ggtattggtc agcctggccg cgatgtgaag cgtatcgccc
     7681 caggccagct gcggcgtgct gagcttcagg tcgctcagcg cgaaggtcgt gtagcccagg
     7741 ccgaagccga acggatacag ggcctcgttg cggatcgagc gccagcgagc cttgtattcc
     7801 tgggcgttcg gatcgaccgg cgcgggccga cccgtagtgc ggccattgta gaagaacggc
     7861 tcctggccgc tctccagcgg gaagctgacc ggcagacggg cggagggatt gaccgcgccg
     7921 aacagcacgt cggcgacagc attgcccatc tcactgccca ggaaccatgt ggccaggatg
     7981 gcgcgggcgt cgcgcaccac gccttccagg gcgatggcgc ggccgtggcg tagcagcacg
     8041 acgacaggct tgccggtggc ggcaatagcc tcggccaggc gctgctgaac gggcgggatg
     8101 cggatatcgg tgcgggactt ggcctcgccg gtcatgtcct ggctttcgcc gatcgccagc
     8161 agcacaacgt cggccgcctg ggccgcggcg acggcgcgct cgaagccgcc ggccagggta
     8221 gtctcgacct cgcagccgcg ctcgacgatc aggctggccg gatcggtcat ggcggcgcgc
     8281 aggccggtgg ccagatccac gttcaggctc ttgtcgccaa agaaggccca cggtccgacg
     8341 acattgtcgc gatcctcggc gaacggacca atcagggcca ggcgctgacc ggcgcgcggc
     8401 aggggcagga tgccgccctc gttgcgcagc agcacaatcg agcggccgcc agcctcgcgg
     8461 ctcagttgct tgagagccgg tgtcgccgtg cgcatcgcct cgaccttggc gttgatcgag
     8521 cgatagggat tgtcgaacag ccccagcgct tccttcaggg ccagcacccg gcgcaccgag
     8581 gcgtccacca ccgccatcgg aacctcgccc gaagcggtca gctccggcag gaagcgcaga
     8641 tagagaccgc tctgcatgct gatatcgacg cccgccagga tcgccaggcg cgccgcgtcg
     8701 cgatcatcgg cggcgaagcc gtgggccacc aattcctggt cggcggtgta gtcggagatc
     8761 accaccccct tgaagcccca ctcgccgcgc aggatgtcgg tcatcaggcg cttgttgccc
     8821 gtcgagggca cgccattgat atcgttgaag gccgacattg tggtcagagc cccggccgca
     8881 aaggcggcct ggaacggcgg cagatgcacc tcgcgcaggg tggcctccga cagttcgacg
     8941 gcgttgtagt ccaggcccgc cgtcaccgcg ccataggcgg cgaagtgctt gggcgtggcc
     9001 agcatgctat cctcggcctt caggtccttg ccctggaaac cgcgcacccg ggcctcggcc
     9061 aggacctggc ccagatagac atcctcaccc gcgccctctg ctacccggcc ccagcgctgg
     9121 tcgcgggcta cgtccaccat gggggcgaag gtccagtgca ggccggacgc gctggcctcc
     9181 gacgcggcgg cccgcgcggt gcgctcgcac agatgcgggt caaaactgcc ggactcggcc
     9241 agcggga
//
LOCUS       DAOMEO010000007         9115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1249_length_9115_cov_2.677815, whole genome shotgun sequence.
ACCESSION   DAOMEO010000007 DAOMEO010000000
VERSION     DAOMEO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_9115_cov_2.677815"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLH31_00320"
     CDS             <1..256
                     /locus_tag="PLH31_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HQR87732.1"
                     /translation="FTTGRGTPLGFPAPTLKIASNSGLATRKPGWIDFDAGVVLSGQS
                     MDDAADALMDLVVETASGKATKAELNGEREIAIWKSGVTL"
     gene            253..1698
                     /locus_tag="PLH31_00325"
     CDS             253..1698
                     /locus_tag="PLH31_00325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HQR87733.1"
                     /translation="MTALPVSSALRLRATSSPAALPGVAQPAYDRDRITVGVVHLGPG
                     AFHRAHQAYYFDQLLTNDPRWGICAVSLKSPGVRDALAPQDGLYTLAQLDEETTFRII
                     GSIVEVLVAPEDPPAVFARLSAPMTRMVTLTVTEKGYCLSSDGGLDESHPDIVHDLAH
                     PREPVSAIGYLVEGLRRRFAVGLPPYAVVACDNLADNGWRLKAAVTAFAAKVDPELAA
                     WIEAEGRFPRTMVDSITPATDDALSARVETATGLNDAWPIQREAFTQWVVEDVLGSDA
                     PDLASVGVTLTDDVRGFERAKLRLLNGVHSTLAYAGILRGHETVFEAISDPVLAKLAR
                     DLMIEDIIPTLTPPRGMDLPAYAEAILKRFRNPEIRHLLAQIAWDGSQKLPFRILGTL
                     GEALDAGRSVDRLAIPLAAWMRFIRLRASAGEKIVDPLADKLVAVAAMCTGDARADVA
                     AFVALDAVFPASLAANPAFVGALEKAYADLG"
     gene            complement(1705..2265)
                     /locus_tag="PLH31_00330"
     CDS             complement(1705..2265)
                     /locus_tag="PLH31_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQR87734.1"
                     /translation="MKRVFDVAAAALGLLVLLAPLAVLWLLVRLTSPGPGLYWSQRVG
                     RGSQLFSMPKFRTMRIDTPEVATHLLDDPDRWLTPIGAPLRRLSLDELPQLWSVLVGD
                     MSLVGPRPALFNQDDLIGARRAAGVDHLRPGVTGWAQINGRDELSIPDKVALDADYLA
                     RRTFGFDLMVLARTVAPVVTGRGVTR"
     gene            complement(2279..>2517)
                     /locus_tag="PLH31_00335"
     CDS             complement(2279..>2517)
                     /locus_tag="PLH31_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87735.1"
                     /translation="GAREVVKRLAPAAIRRLVRRLFSDATLRANAERFLKRYAGMIEE
                     AGGQDREGFLVAALLSSDAGRAYLLLDAATGDLA"
     assembly_gap    2518..2527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2528..4802)
                     /locus_tag="PLH31_00340"
     CDS             complement(<2528..4802)
                     /locus_tag="PLH31_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polar localization protein TipN"
                     /protein_id="HQR87736.1"
                     /translation="MSETDLDLEIAAPEPSSPRARAASRRGRISEPSQASFEPTPPSA
                     TSLATSAAQPPAPVVEPERETAPARAETREASADLRRPTEAFELPDRSERGPMPAFLF
                     WSFAVAAAALWALAPIAFALGYARDVPALKVEGFALAVFAGLAIGPALLTLLGAYLLR
                     QALGVSAELRRTRTLTDRLVTPAVLAAAGASGAVEAIRGQIDSATIAADEARERILSL
                     RQSLAEETARLADAAADSARMANQLAQGLGHERQALETLSLTLDTRSTAVVDAIGSQA
                     RMVAEASDLAETQLREAEAALAARAADLAAAAAEASDAARVGAEDISRQIARLETAGQ
                     GLGDQMSAVEKNLAQQRAALVAASQSLRSEQEDFAAETETRTAQLTEFVSYTRVGATE
                     LSDVAAMGAEVLRGLISAASEQFKAMADNAKAERDSLVEDTNRVLTAISDAAAGQREG
                     LEIELKQAMEALAASALKASEGVDARVDSARGRVDQLNEIAFAAGQKADTVFEARMEE
                     ARDLIEQSAQMVEQAGARTAQKLAEAVLTARGTLGELEAMLDEVGQRTAEMPAEALQK
                     AVEVRASIEQGLEDLMAAARRTAEETAAIDQAFQDRVRRNYDALSEAAAAMGGSTPAR
                     PSPPPLVRAKVAPAPAAPPPPPPPIAPQPLAEPEDDLLAEEPGHRPRLRLTPTATDDE
                     FRSVFDTAGGRGAPPAAPEGPDGQGWSWRSLLSGIENSSPGDAALGEKLAAEISAMGI
                     DPHALLPRGRIDEIAAAI"
     gene            complement(5104..5298)
                     /locus_tag="PLH31_00345"
     CDS             complement(5104..5298)
                     /locus_tag="PLH31_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87737.1"
                     /translation="MSLLAEILAVAVVWLSSVALGQFGIVLDTCPLAKTTSERTVSRS
                     PRAAPSPIAIAPQTAPLPNA"
     gene            complement(5386..6552)
                     /locus_tag="PLH31_00350"
     CDS             complement(5386..6552)
                     /locus_tag="PLH31_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR87738.1"
                     /translation="MSSLTRLSFFYVAIYLGTGVSLPYIATYLRDRGLSGAAIGLILA
                     IPMLAKPFTGPALAVWADGFTLRRSPMVLLSLGAGLGYAALLIAPGALGLAIAWFIGQ
                     TLLSTVSPLLDVVTLRRARSEGFNYGLPRGTGSAAFIVANLVMGVALTFAAPAIIPLW
                     IALSALLAALAAGVFVPPERVHAEGAKPLGSERWKDLGALLRDRTFVLAVMTVGLIQG
                     AHAFYYGFSTILWRNQGISEPIIGLLWGVGVAAEVGFMWFLEPWRRKVGPEKLLILGA
                     VAAVLRWIAYAFAPPLWMLFPMQALHALTFAATFMASLRLIEKLTQPANASAGQALNS
                     AFSGGFTLGVATLCAGPLFDAFGSYGYLAMAVTAALGLAGAIVLHRRTGSLQPS"
     gene            complement(6599..8314)
                     /gene="metG"
                     /locus_tag="PLH31_00355"
     CDS             complement(6599..8314)
                     /gene="metG"
                     /locus_tag="PLH31_00355"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQR87739.1"
                     /translation="MARILITSALPYINGIKHLGNLAGSMLPADVYARFQRARGHETL
                     YICATDEHGTPAELAAAAAGQDVETYCAEQHILQRDVAAAFGLSWDWFGRSSSPQNRK
                     LTQHFAEVLEANGLIEERVDQMVYSIDDKRFLPDRYIEGTCPHCGFEKARGDQCDNCG
                     NLLDPTDLKNAYSVISGSRNLEVRDTRHLYLLQTRMADRIRTWVDGHGDWPVLAKSIA
                     YKFLDEGLIDRGITRDLAWGVPVVKDGAPRPGMEGKVFYVWFDAPIEYIAATQEWADA
                     EEGRDWKRWWRTDAGAQDVRYVEFMGKDNVAFHTVSFPATILGSEEPWKSVDMLKAFN
                     WLNWYGGKFSTSSKRGVFMDAALEILPPDFWRWYLTSNAPESSDTAFTWEMFASSVNR
                     DLADVLGNFINRILKFTEGKFDGVVPEGGEPGPLEEKLFADVSARLADLAEQMDAIEV
                     RKSAQALRALWVVGNEYLQEAAPWTAIKTDRDRAAVIVRTALNLAALYARISTPFIPF
                     AAEKIAQAFSLEFPAAWPSNDAAVELSSLPTGLSVRAPEVLFKKIDEEMIAEWTARFG
                     GAEEG"
     gene            8483..8896
                     /locus_tag="PLH31_00360"
     CDS             8483..8896
                     /locus_tag="PLH31_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HQR87740.1"
                     /translation="MKTINWLGLVAALVVGQVIGIVWYGSLFSAAWQEAMEMTQADFA
                     GTEWRMSLGLVNMVVILIGLDWLIGRLDARSWMAGARVALAACIFFALTVVSLDYIYA
                     AGNLPLLWIDGGYQLLTYVIGGALLGGLRLKPKAP"
     gene            8972..9047
                     /locus_tag="PLH31_00365"
     tRNA            8972..9047
                     /locus_tag="PLH31_00365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9005..9007,aa:Thr,seq:tgt)
BASE COUNT         1519 a         3086 c         3092 g         1408 t
ORIGIN      
        1 gttcaccact ggacgcggta cgccgctggg cttccccgcg ccgacgctga agatcgcctc
       61 caacagcggg ctggcgacgc gcaagccggg atggatcgac ttcgacgccg gcgtggtgct
      121 gtctggccag tcgatggacg acgcggccga cgcgctcatg gacctggtcg tcgaaacggc
      181 gtctggcaag gccaccaagg ccgaactcaa cggcgaacgt gaaatcgcca tctggaaaag
      241 tggtgtgacc ctgtgacggc tcttcccgta tcctctgcct tacgtcttcg cgccaccagc
      301 agccctgctg cgctcccggg cgtggcccag ccggcctatg accgcgaccg gatcaccgtc
      361 ggcgtggttc accttggccc gggcgcgttt caccgcgcgc accaggccta ttatttcgac
      421 caacttctga ccaacgaccc gcgttggggc atctgcgccg tctcgctgaa gagcccgggc
      481 gtgcgcgacg cgctcgcgcc gcaggacggc ctctacaccc tggcccagct ggatgaggag
      541 accaccttcc ggatcatcgg ctccatcgtc gaggtgctgg tcgcgcccga ggatccgccg
      601 gccgtcttcg cccgcctttc agctcccatg acccgcatgg tcaccctgac cgtgaccgaa
      661 aagggctact gcctgtccag cgacggcggc ctggatgaga gccatcccga catcgtccac
      721 gacctggccc atccccgcga acccgtcagc gccatcggct atctggtcga gggattgcgc
      781 cgacgcttcg cggttggcct gccgccctac gcggtcgtcg cttgtgacaa cctcgccgac
      841 aacggctggc gcctgaaggc ggccgtcaca gcgttcgccg ccaaggtcga tcccgaactc
      901 gccgcctgga tcgaggctga aggccgcttc ccgcgcacga tggtcgacag catcaccccg
      961 gccaccgacg acgccctgag cgcgcgggtc gaaacagcca cgggcctgaa cgacgcctgg
     1021 cccatacagc gcgaagcctt cacccagtgg gtggtcgagg acgttctggg ttcggacgcc
     1081 ccggaccttg ccagcgtcgg cgtcaccctg accgacgacg ttcgcggctt cgagcgggcc
     1141 aagctgcgcc tgctgaacgg cgtgcattcc accctggcct atgccggaat cctgcgcggc
     1201 catgaaaccg tgttcgaagc gatcagcgac ccggtcctgg ccaagctggc gcgtgatctg
     1261 atgatcgagg acatcatccc caccctgacg cccccgcgcg gcatggacct gccggcctat
     1321 gctgaggcga tcctgaagcg cttccgcaat ccggagatcc ggcacctgct ggcccagatc
     1381 gcctgggacg gctcgcaaaa actgccgttc cggattctgg gcacgctggg cgaggcgctg
     1441 gacgccggac gctcggttga tcgcctggct ataccgctgg cggcctggat gcgcttcatc
     1501 cgcctgcggg cctcggcggg tgaaaagatc gtcgacccgc tggccgacaa gctggtcgcg
     1561 gtcgcggcga tgtgcacggg cgacgccagg gcggatgtcg cggcgttcgt ggcgctggat
     1621 gccgtatttc cggccagcct ggcggcgaac cccgccttcg ttggcgccct tgagaaggcc
     1681 tatgccgatc tgggctagat cgaactagcg cgtgacgccc cggccggtga cgaccggggc
     1741 gaccgttctg gccaaaacca tcagatcgaa cccgaacgtg cgccgcgcca gatagtcggc
     1801 gtccagcgcc accttgtcgg gaatggacag ctcgtcccgc ccgttgatct gggcccagcc
     1861 cgtgacgccg gggcgaaggt ggtcgacgcc ggcggcccta cgcgcgccga tcaggtcgtc
     1921 ctgattgaac agggccggtc tgggaccgac cagactcatg tcgccgacca gcacactcca
     1981 aagctggggc agctcgtcca ggctgaggcg acgcaacggc gcgccgatgg gggtcagcca
     2041 gcggtcggga tcatcgagca gatgggtcgc aacttccggc gtatcgatcc gcatcgtccg
     2101 aaacttcggc atcgagaaca gctgcgagcc ccggccgacc cgctgcgacc agtagagccc
     2161 cggtcccggc gaggtcaggc ggaccagcag ccaaagcacg gccagcggcg ccagcaggac
     2221 cagcagtccc agggcggcgg ccgcgacgtc gaagacgcgc ttcagcatcg gcctgcgatc
     2281 aggccaggtc gccggtggcg gcgtccagta gcagataggc gcggccagcg tccgacgaca
     2341 gcagcgcggc cacgaggaag ccttcgcggt cctggccgcc agcttcctcg atcatccccg
     2401 catagcgctt gaggaaacgc tcggcattgg cccgcagggt cgcgtccgag aacaggcgtc
     2461 gcaccagccg gcggatggcg gccggggcca ggcgcttgac cacttcgcgg gcgccggnnn
     2521 nnnnnnngga tggcggcggc gatctcgtcg atccggccgc gcggcagcag ggcatgcggg
     2581 tcgatgccca tcgccgagat ttcggcggcg agcttttcgc ccagggccgc atcgcccggc
     2641 gagctgttct cgatccccga cagcagcgag cgccaggacc agccctgtcc gtccggtcct
     2701 tctggcgcgg caggcggagc gccccggccg ccggcggtgt cgaacacgga gcgaaactcg
     2761 tcgtcggtgg cggtcggcgt caggcgcaaa cgcggccggt ggccgggctc ctcggccagc
     2821 agatcgtcct ccggttcggc cagcggctgc ggcgcgatcg gcggcggcgg cggcggcggc
     2881 gctgcgggag ccggcgcaac cttggccctg acaagcggcg gcgggctggg acgggccggc
     2941 gtcgatccgc ccatggcggc ggcggcttcg ctgagcgcgt catagttgcg gcgtacgcga
     3001 tcctggaagg cctggtcgat cgccgccgtt tcttcagccg tgcggcgcgc cgcagccatc
     3061 aggtcttcga ggccctgctc gatggaggcc cgaacctcga cggccttctg cagggcctcg
     3121 gccggcattt cggcggtgcg ttggccaacc tcgtccagca tcgcctcaag ctcgcccagg
     3181 gtgccgcgcg cggtcagaac cgcctcggcc agcttctggg ccgtacgggc tccggcctgt
     3241 tcgaccatct gggccgactg ctcgatcaga tcgcgcgcct cttccatgcg cgcctcaaag
     3301 acggtgtcgg ccttctggcc ggcggcaaag gcgatctcgt tgagttgatc gacacggcca
     3361 cgcgccgagt cgacgcgcgc atcgacgccc tcgctggcct tcagcgccga agccgcaagg
     3421 gcttccatcg cctgcttgag ctcgatctcc agaccctcgc gctgtccggc ggcggcgtcg
     3481 gaaatggcag tgagcaccct gttggtatct tccaccagac tgtcacgctc ggccttggcg
     3541 ttgtcagcca tggccttgaa ctgctcggag gcggccgaga tcaggccgcg cagcacctcg
     3601 gcccccatgg ccgcgacatc gctgagctcg gtcgcgccga cccgggtgta ggagacaaac
     3661 tcggtcagct gcgccgtgcg ggtttccgtc tcggcggcga agtcttcctg ttcggaccgc
     3721 agcgactggc tggccgcaac cagcgccgcg cgctgctggg ccaggttttt ctcgacggcg
     3781 ctcatctggt cgcccagacc ctggccggcg gtttccagcc gcgcgatctg gcgcgagatg
     3841 tcttcagcgc cgacgcgcgc ggcgtcagag gcttcggcgg cggcggcggc cagatcggcg
     3901 gcgcgcgccg ccagggcggc ctcggcttcg cgaagctggg tttcggccag gtcagaggcc
     3961 tcggcgacca tccgcgcctg actgccaatg gcgtcgacaa cagcggtgga ccgcgtgtcc
     4021 agggtgaggg acagggtctc cagggcctgg cgctcgtggc caagcccctg cgccagctgg
     4081 ttggccatgc gggcagagtc ggccgcggca tccgcgagac gcgcggtctc ctcggccagc
     4141 gactggcgca gcgacaggat acgttcgcgc gcctcgtcgg cggcgatggt ggcgctgtcg
     4201 atctggccgc gaatggcttc cacggcgcca gaagcgcccg cagccgccag aacggctggc
     4261 gtcaccagac gatcggtaag ggtccgcgta cggcgcagct cggccgaaac gcccagagcc
     4321 tggcggagca gataggcgcc cagcaaggtg agcaacgcag ggccgatcgc cagaccggca
     4381 aagaccgcca gggcgaagcc ttcaaccttc agggccggga cgtcgcgggc atagcccagc
     4441 gcgaaggcga tcggcgccaa ggcccaaagc gcggctgcgg cgacggcgaa tgaccagaac
     4501 aggaaagcgg gcatggggcc gcgctcggaa cggtccggca gctcgaaagc ctctgtgggc
     4561 cgtcgcaagt ctgccgaggc ttcgcgcgtt tcagcgcggg ccggcgcggt ctcgcgctcg
     4621 ggctcgacga caggtgcggg cggctgagcg gcgctggtcg ccagagacgt cgcgctgggc
     4681 ggcgtcggtt cgaacgaagc ctgggagggc tcgctgatcc gccctcgacg cgacgcggcc
     4741 cgcgcccggg gcgatgaagg ctccggcgcc gcgatctcga gatcaaggtc ggtttcgctc
     4801 attggcggat cgacagccgc ggcggcgtca gcctccgcct cgtcctcggc aaccgaggct
     4861 tcgggcgagg agacttgatc ctctacgggc gcctccacgg ggacggcgga gaaatcgagc
     4921 ggttggcgct tcttaggctt catacacagc cagggtccca aggcggtcag cgaagggcga
     4981 agacagtcaa cgcgcgccct gcccccgcgt agagaatgaa cctagcggcg aaacgcccgc
     5041 gcgcaaagcg gcttcggttg agttttaagg gcttcacgtc gtcgcagcgc gccgagcgca
     5101 gcttcaggcg ttcggcaggg gagcggtttg cggcgcaatg gcgatggggc tcggcgcggc
     5161 ccgcggggat cgcgacacgg tgcgctcgga cgtcgtcttg gccaggggac aggtatcgag
     5221 cacgatgccg aactgaccca gcgccacgga ggacaaccag acaacggcga cagccagtat
     5281 ttccgccagc aagctcatgg cccgacccat agtgcgatag cggtgttatg cgatagactc
     5341 gcgccttagc cgagtgaact tttggtcatg atggcaagca tcccgctagg atggctgcag
     5401 gctgccggtg cggcggtgga gaactatcgc gcccgccagg cccagagccg ccgtcaccgc
     5461 catggccaga tagccgtacg atccgaacgc atcgaacagc ggccccgcgc agagggtcgc
     5521 cacgcccagg gtaaacccgc ctgaaaaggc cgagttgagg gcctgaccgg ccgaggcgtt
     5581 ggcgggctgt gtcagtttct cgatcagacg cagcgacgcc atgaacgtgg ccgcgaaggt
     5641 cagggcgtgc agggcctgca tcggaaacag catccagagc ggcggcgcaa aggcataggc
     5701 gatccagcgc agcaccgccg cgacggcgcc gaggatcagc agcttctcgg gacccacctt
     5761 gcggcgccag ggctccagaa accacatgaa gcccacctcg gcggcgacgc cgacgcccca
     5821 cagcaggccg atgatcggtt cagaaatccc ctggttgcgc cagaggatgg tcgagaaacc
     5881 gtagtagaag gcgtgggcgc cctggatcag accgacggtc atcaccgcga gcacaaaggt
     5941 cctgtcccgc aacagcgccc ccaggtcttt ccagcgttcc gatcccaacg gcttggcccc
     6001 ctcggcgtgg acccgttcgg gcggtacgaa aacgccggcc gccaaggccg cgagcaaggc
     6061 cgacagggca atccagaggg gaatgatcgc cggcgcggcg aaggtcagcg cgacccccat
     6121 caccagattg gcgacgatga aggcggcaga gcccgtcccg cgcggcagac catagttgaa
     6181 gccttcggac cgcgcccggc gcagggtgac cacgtcgagc aatggcgata cggtcgacag
     6241 cagagtctgc ccgatgaacc aggcgatcgc caggccaagg gcgcctggcg cgatcagcag
     6301 cgccgcatag ccaagcccgg cgcccagcga cagcagcacc atgggcgagc ggcgcagggt
     6361 gaagccgtcg gcccataccg ccaaagccgg gccggtaaac ggcttggcca gcatcggtat
     6421 ggccaggatc aggccaatgg ccgcgccgct caggccccga tcgcgcagat aggtggcgat
     6481 atagggcagg ctgacgcccg tccccagata gatcgcgacg tagaaaaagc tgagccgcgt
     6541 caggctgctc atcgcgctct accgcccgag caggggccgc ctatgccgcg cttcgggatc
     6601 acccctcttc agccccgccg aagcgagccg tccattcggc gatcatctcc tcgtcgatct
     6661 tcttgaagag gacctccggc gcgcggaccg acaggccggt cggcagcgaa gacagttcga
     6721 ccgccgcgtc gttcgacggc cacgcagccg ggaactccag cgagaaggcc tgggcgatct
     6781 tttccgccgc gaacgggata aagggcgtgg agatcctggc gtacagggcc gccagattca
     6841 gggccgtgcg cacgatcacg gcggcgcggt cgcggtcggt cttgatcgcg gtccacggcg
     6901 cggcttcctg caggtactcg ttgccgacca cccagagcgc ccgcagcgcc tgggcgctct
     6961 tgcgcacctc tatcgcgtcc atctgttcgg ccagatcggc caggcgcgcc gaaacgtcgg
     7021 cgaacagctt ctcttccagc ggaccaggct cgccgccctc ggggacgacg ccgtcgaact
     7081 tgccttccgt gaacttcagg atgcggttga tgaagttgcc cagcacgtcg gccaggtcgc
     7141 ggttcaccga gctggcgaac atctcccagg tgaaggcggt atccgagctc tcgggcgcgt
     7201 tcgaggtcag gtaccagcgc cagaagtcgg gcggcaggat ttccagcgcc gcgtccatga
     7261 acacgccgcg cttggagctg gtcgagaact tgccgccata ccagttgagc cagttgaagg
     7321 ccttgagcat gtcgacgctc ttccatggct cttccgagcc caggatcgtc gccgggaagc
     7381 tgacagtgtg gaacgccacg ttgtccttgc ccatgaactc gacataccga acatcctgcg
     7441 cgcccgcgtc ggtgcgccac caacgcttcc agtcgcggcc ctcttcggcg tcggcccatt
     7501 cctgggtagc ggcgatatat tcgatcggag cgtcgaacca gacatagaag accttgcctt
     7561 ccatgcccgg acgcggcgcg ccgtccttga ccaccggtac gccccaggcc aggtcgcggg
     7621 tgatgccgcg atcgatcagg ccctcgtcca ggaacttgta ggcgatcgac ttggccagga
     7681 ccggccaatc gccatggccg tcgacccagg tccggatcct gtcggccatc ctggtctgca
     7741 gcaggtaaag gtggcgagtg tcgcgcactt ccaggttccg cgagccggag atcaccgaat
     7801 aggcgttctt caggtcggtc ggatccagca ggttgccgca attgtcgcac tggtcgccac
     7861 gcgccttttc gaagccgcag tgcgggcagg ttccctcgat atagcggtcg ggcaggaagc
     7921 gcttgtcgtc gatggaatag accatctggt cgacgcgttc ctcgatcagg ccgttggcct
     7981 caagaacctc ggcgaagtgc tgggtcagct tgcggttctg cggtgaggac gagcgcccga
     8041 accagtccca cgacaggccg aacgcggcgg cgacgtcacg ctgaagaata tgctgctcgg
     8101 cgcagtaggt ttccacgtcc tggcctgcgg cggcggctgc cagctcggcg ggagtcccat
     8161 gttcatcggt ggcgcagata tagagcgttt catggccccg ggcccgctgg aaccgcgcat
     8221 agacgtcggc cggcaacatc gatcccgcca gattgcccag gtgcttgatg ccgttgatgt
     8281 acggcagggc ggaggtgatc aggatacgag ccatgaggcg aagtcttccg gggagtcaga
     8341 gatcaaggaa acaggcgttc gcatatagaa ggctgggccg cgaaacaaaa gcgccgggcc
     8401 gttgaccaaa cgccgttatg cggcttccct tcccttgggt gagccggaca agacgatccg
     8461 gccccatcgg gggatagcgc ttatgaagac catcaactgg ctgggcctcg tcgcggccct
     8521 ggtcgtcggc caggtcatcg gcatcgtctg gtacggctcg ctcttcagcg ccgcctggca
     8581 ggaagcgatg gaaatgacgc aggctgactt cgccggaacc gagtggcgga tgagcctggg
     8641 cctcgtcaac atggtggtca ttctgatcgg cctggactgg ctgatcggcc gtctggacgc
     8701 ccgcagctgg atggccggcg ccagggtcgc cctggccgct tgcatcttct tcgccctgac
     8761 ggtggtcagc ctcgactaca tctatgcggc cggcaacctg ccgctgctgt ggatcgacgg
     8821 cggctatcag ctactgacct atgtgatcgg cggcgcgctg ctgggcggcc tgaggttgaa
     8881 gccgaaagca ccttagtcgc cgacgaccaa agccattgcc cggcggcgat ccggacgcta
     8941 aggaggccac cgtccgcttc aagcggcggt ggccggttta gctcagctgg tagagcagcg
     9001 gttttgtaaa ccgaaggtcg cgggttcgat tcctgcaacc ggcaccactc tcctaagacg
     9061 ccttggtctc ggcgcccagg aacaccacct tggcgccctt ctggacggcc gcgcc
//
LOCUS       DAOMEO010000008         8922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1270_length_8922_cov_2.838841, whole genome shotgun sequence.
ACCESSION   DAOMEO010000008 DAOMEO010000000
VERSION     DAOMEO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_8922_cov_2.838841"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /gene="lpdA"
                     /locus_tag="PLH31_00370"
     CDS             complement(<1..1332)
                     /gene="lpdA"
                     /locus_tag="PLH31_00370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQR87741.1"
                     /translation="MTETLRPKVLVIGGGPGGYVAAIRAGQLGLDTVLVESGRLGGTC
                     LTRGCIPSKALIHAAGLYEEAVLASAETGRFGIHLTQAPTLRFEETVGWKDAIVDRLS
                     GGVGGLLRKAKVKVVTGWATFSDAKTCIVETAKGPVKIQPEHVILATGSTAVELPFLP
                     FGDRVISSTEALSLSEVPATLAVVGGGYIGLELGIAFAKLGSKVTIVEATDRLLPLYD
                     TQLTAPVSRWLEKHGVTVHLGAKALGQDDEGLLIQTAGGKSQLVVPAERILVTVGRKP
                     LTEGWGLDQMALAMDGRFVKVDDRCATSTTNVWAIGDLVGEPMLAHKASAQGEMVAEI
                     IAGRRRRFDPVAIAAVCFTEPEIVSVGLTPDQAPEGVETIVGQFPFQANGRALSMQAG
                     DDGGFVRILARKSDHRILGIQAVGRHCSEFAGEFAALMEMGAVLEDVAGVIH"
     gene            complement(1337..2611)
                     /locus_tag="PLH31_00375"
     CDS             complement(1337..2611)
                     /locus_tag="PLH31_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQR87742.1"
                     /translation="MGLYLFRLPDIGEGVAEAEIVAWRVKVGDVVAEDQDLAEVMTDK
                     ATVEISSPVAGTVLAIHGEIGGMRPVGAVLVELESDAGSDVAEVVAAPAAPAPVQPQV
                     LAQPLATATPPAPVKGPAPASTARPRTSSGRGAGEAPLAAPSTRRRALDLGISLAQVP
                     GSGPGGRITPDDLDAFLVQDAKAGGGSSLVARDGINDTRIIGLRRKIAEKMQEAKRRI
                     PHINYVEECDLTELEALRLDLNEHRADDQPKLTLLPFIMRAMVKALPEFPQINALYDD
                     DNGVLHAHEGVHIGIATQTPNGLIVPVVRHAEARDIWDCAREVARLAKAVRDGSATRD
                     ELSGSTITLTSMGPLGGIVSTPVINHPEVAILNPNKLVDRPMVQGSFITVRKMMNLSS
                     AFDHRIVDGYDAALFVQRVKRLLEHPALIFMD"
     gene            complement(2615..3628)
                     /locus_tag="PLH31_00380"
     CDS             complement(2615..3628)
                     /locus_tag="PLH31_00380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQR87743.1"
                     /translation="MPTMNMIQALNSALDVMLTEDPDTLIFGEDVGYFGGVFRVTDGL
                     QKKHGLTRCFDAPISEGGIIATAIGMGAYGLRPIPEIQFADYILPAFDQLVSEAARLR
                     YRSNGEFWAPITVRSPYGGGIFGGQTHSQSPEAIFAHITGLKTVIPSNPYDAKGLLIA
                     SIEDDDPVIFLEPKRLYNGPFDGRHEQALKTWAGDPTAEVPSGRYTVPLGKAAIVREG
                     AEATVLAYGTMVHVALAGIEDSGVDAELIDLRSIVPLDVETIVASVKKTGRCVILHEA
                     SRFGGFGGELSALVQERCFYHLKSAVQRVAGWDTPYPHAFEWDYFPGPARLAAALKRA
                     MED"
     gene            complement(3633..4862)
                     /locus_tag="PLH31_00385"
     CDS             complement(3633..4862)
                     /locus_tag="PLH31_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR87744.1"
                     /translation="MNNRPPLRLHIPEPDARPGDTPAFDRALIPEPGDTRRPEASTLE
                     AEVRDLPYGLIRVLDDSGEAKGPWNPNLPTNTLLAGLRAMLLTRVFDERLFRAHRQGK
                     TSFYMKSTGEEAIGAAQSLFLDRDDMCFPTYRVLSWLMARNYPLIDLCNQIFSNEKDP
                     LKGRQLPILYSARKYGFYSLSGNVGSRFGHAVGWAMASAFKGGDSIALAYIGEGTTAE
                     GDFHEALTFASVYKAPVILCVTNNQWAISSFSGIAGANETTFAAKALAYGLPGLRVDG
                     NDFLAIWAATEWAAERARQNLGATLIELYTYRASGHSTSDDPTKYRPADEAEAWPLGD
                     PVERLKTHLIGLDAWDEDRHAALIAELDAEVRAAVKEAEAVGTLGKSKPSVKEMFEGV
                     FKDPDWRVTEQRRELGI"
     gene            4893..5531
                     /locus_tag="PLH31_00390"
     CDS             4893..5531
                     /locus_tag="PLH31_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR87745.1"
                     /translation="MPSDIRNKIVYLERYTEPYALADAPLAKIVASRCALRHKVGSAQ
                     SKRASPPSGFFMASPPDLDETDLKILDVVQRNAPMTTAELADRVGMSQSPCWRRLTRL
                     KEEGYIVDQVTRLSAEKLGLRTNVFANVKLSAHGQSNLNEFIEAVSRIPEVLEIHPTM
                     GPFDFLLRIITRDVEDYRDLAFGKLLTLPTVQEINSTMSLGPVKMTTALPIR"
     gene            complement(5695..6087)
                     /locus_tag="PLH31_00395"
     CDS             complement(5695..6087)
                     /locus_tag="PLH31_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQR87746.1"
                     /translation="MFARAERRAKRAGPKPSGEPVARPPSPFSQALQRLGLTATMVRH
                     WEEAGIVEFKRVGGRRIIDDNALECLTTILQLRRAGFTIRQITWTSDILPPTVSAMRH
                     ALEARQGLTEIARATTIARAIVTGRNAT"
     gene            complement(6071..6547)
                     /locus_tag="PLH31_00400"
     CDS             complement(6071..6547)
                     /locus_tag="PLH31_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQR87747.1"
                     /translation="MIPSIAPSRPIRLRSSVLVPINVLAARLGLSHRALRHYEALGLV
                     HSERDFGNTRHYDSQAVATLEVIAFLRRADIAIPVIRSIIALRHDRPRYLQAIREALI
                     DAAEHKERDLAALREALVDLESASAAFQPQEEAGNDPSQRLCGTDDQVGPSDVRPS"
     gene            complement(6544..8538)
                     /locus_tag="PLH31_00405"
     CDS             complement(6544..8538)
                     /locus_tag="PLH31_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HQR87748.1"
                     /translation="MFSKILIANRGEIAVRVIKTCRRLGIATVVVYSDADAGSMAVEM
                     ADEAVHIGPSPANQSYLVADKIIDACKQTGAQAVHPGFGFLSENAGFAQRCADEGIAF
                     IGPNPGAIRAMGDKIESKKFAQAAGVSCVPGHIGEIDDTAHAVKISEEIGYPVMIKAS
                     AGGGGKGIRVAWTRQDVEEGFPAVRAEAKASFGDDRIFIEKFIVSPRHIEIQVLGDKH
                     GNVVHLFERECSIQRRNQKVIEEAPSPLLDEATRNAMGAQAVALAKAVNYDSAGTVEF
                     VAGQDKSFFFLEMNTRLQVEHPVTELITGLDLVEQMIRSAWGEKLAFAQKDLKINGWA
                     IESRIYAEDPYRKFLPSIGRLVRYAPPEEGQHEGYVVRNDAGVREGDEISMFYDPMIS
                     KLCTWAPTRIAAVDGMGRALEDFHIEGLGQNIPFLNAVMDQERFRSGQLSTNYIKDEF
                     PDGFTGTEPTPAQIDILIVVGAAMQRVYAARARSNAVVFGGPVRDEWVVAVGHAKRRV
                     KLAGQGTLSVELLDEGRTLTLSAIDWRPGLPTFRGELDGKPFTVQVAPAAEGFTVRHR
                     AAKARVLVLTPRSAELHDKLPEKQAADTSKLVLSPMPGLVVSMDVTAGQQVREGEVVC
                     VLEAMKMQNIIRAERDGLVKAVNAKGGDPVAADEVLVEFA"
     gene            complement(8837..>8922)
                     /locus_tag="PLH31_00410"
     CDS             complement(8837..>8922)
                     /locus_tag="PLH31_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87749.1"
                     /translation="RRIVRGLKSLKGKELSNPWKKHDNIPL"
BASE COUNT         1494 a         2929 c         2918 g         1581 t
ORIGIN      
        1 gtggatgacg ccggcgacgt cttccagaac ggctcccatc tccatcaggg cggcgaactc
       61 gcccgcgaac tccgagcagt gtcgtccgac cgcctggatg ccgagaatcc gatggtcgga
      121 ttttcgcgcc aggatgcgca cgaacccgcc gtcgtccccg gcctgcatcg acagggcgcg
      181 gccgttggcc tggaaaggga actgacccac gatggtctcg acgccttcgg gggcctgatc
      241 gggcgtcagt ccgacgctga cgatctctgg ctcggtgaaa cagacggcgg cgatggcgac
      301 cgggtcgaat cgtcgccggc gacccgcgat gatctcggcg accatctcgc cctgcgccga
      361 cgccttgtgc gccagcatcg gttcaccgac cagatcgccg atggcccaga cgttggtggt
      421 ggacgtcgcg cagcggtcgt cgaccttcac gaagcgcccg tccatcgcca gcgccatctg
      481 gtccagcccc cacccttcgg tcagcggctt gcggccgacc gtcaccagga tgcgctccgc
      541 cggcacgacc agctggctct tgccgcccgc cgtctggatc agcaggcctt cgtcgtcctg
      601 gcccagggcc ttggcgccca gatggaccgt gacgccgtgc ttttcgagcc aacgcgacac
      661 tggcgcggtc agctgggtgt cgtaaagcgg cagaaggcga tcagtcgcct cgacgatggt
      721 caccttcgac cccagcttgg cgaaggcgat gccgagctcc aggccgatat agccaccgcc
      781 gaccacggcc aaggtcgccg gaacttctga cagggacagc gcctcggtcg aggagatcac
      841 ccgatctccg aacggcagga acggcagttc gacggcggtg gagcctgtgg ccaggatcac
      901 atgttccggc tggattttga cggggccttt cgcggtctcg acgatgcagg tcttggcgtc
      961 ggagaaggtc gcccagccgg tcacgacctt caccttcgcc ttgcgcaaca gtccgccaac
     1021 gccgccgctg aggcgatcga cgatggcgtc cttccagccg acggtctctt cgaagcgtag
     1081 ggtcggcgcc tgggtcaggt gaatgccgaa ccgtcccgtc tcggcgctcg ccaatacggc
     1141 ttcctcgtag aggccggcgg catggatcag ggccttggac ggaatacagc ctcgcgtcag
     1201 gcaagtgccg cccagccgcc cactctcgac cagcacggtg tccagcccca gttgccctgc
     1261 gcggatggcg gccacgtagc cgcccggccc cccgccaatg acgaggacct tgggtctcag
     1321 tgtctcggtc atcgcatcag tccatgaaga tcagggcggg gtgttcgagc agccgcttca
     1381 cgcgctggac gaacagggcg gcgtcgtagc catcgacgat gcggtgatcg aaggccgagg
     1441 acaggttcat catcttgcgc accgtgatga acgatccctg gaccatcggc cggtccacca
     1501 gcttgttggg attgaggatg gcgacctcgg ggtgattgat gaccggcgtc gaaacgatgc
     1561 cgcctagggg tcccatgctg gtcagggtga tggtcgagcc ggacagttcg tccctggtcg
     1621 ccgagccgtc gcgcacggcc ttggccaacc gcgcgacctc gcgggcgcaa tcccagatgt
     1681 ctcgcgcttc ggcgtgacgc acgaccggaa cgatcagccc gttcggcgtc tgggtggcga
     1741 tgccgatatg cacgccttcg tgcgcatgca gcacgccgtt gtcgtcgtca tagagggcgt
     1801 tgatctgcgg gaattcgggc agagccttga ccatggcccg catgatgaag ggcaacaacg
     1861 tcagcttcgg ctgatcgtcg gcgcggtgtt cattgaggtc gagccgaagc gcctccagct
     1921 ccgtcagatc gcattcctcg acatagttga tgtgcgggat gcggcgcttg gcctcctgca
     1981 tcttctcggc gatcttgcgg cgaaggccga tgatgcgcgt gtcgttgatc ccgtcgcggg
     2041 cgacaagaga cgatccgccc ccggcctttg cgtcctggac caggaaggcg tcgaggtcgt
     2101 cgggcgtgat ccgccctcca ggcccactgc ctggcacctg ggccagcgat atacccaggt
     2161 ccagcgcgcg cctccgggtt gacggcgcgg ccaagggggc ctcccccgct cctctgccgg
     2221 acgaagtcct cggtcgagcc gtgctggctg gcgccggccc cttcacgggc gctggcggcg
     2281 tcgcagtcgc caagggctgg gcgagaacct gcggctggac aggcgccggc gcggcgggtg
     2341 cggccacgac ctcggccacg tcgctcccgg cgtcgctctc aagctcgacc aatacggcgc
     2401 cgaccggccg catgccgccg atctcaccgt ggatcgccag cacggtcccc gcgaccggcg
     2461 aactgatctc gacggtggcc ttgtcggtca tgacttcggc caagtcctgg tcttccgcga
     2521 cgacatcgcc gaccttgacc cgccaggcga cgatctcagc ctcggccacg ccttcgccga
     2581 tgtccggcag ccggaacaga tagagcccca tcgcctagtc ctccatcgcg cgcttgagcg
     2641 ccgccgcaag ccttgcgggc ccggggaaat agtcccactc gaaggcgtgt gggtaaggcg
     2701 tgtcccaacc ggcgacccgc tgcaccgcgc tcttcaggtg atagaagcag cgttcctgaa
     2761 cgagcgccga gagctcgccg ccgaaaccgc caaaccgcga cgcctcatgc aggatgacgc
     2821 accggccggt cttcttgacc gaggcgacga tcgtttcgac gtccagcggc acgatcgagc
     2881 gcaggtcgat cagttcggcg tccacgccgc tatcctcgat cccggccagc gcgacatgga
     2941 ccatcgtgcc ataggcgaga acggtcgcct cggcgccctc gcgcacgatg gccgccttgc
     3001 ccagcggcac ggtatagcgc ccggacggaa cctccgccgt cggatcaccg gcccaggttt
     3061 tcagcgcctg ctcgtggcgt ccgtcgaacg ggccgttata gagccgcttg ggctccagaa
     3121 agatcaccgg atcgtcgtcc tcgatggagg cgatcagcag gcctttggcg tcatagggat
     3181 tggacggaat aacggtcttc aggcccgtga tatgggcgaa gatcgcttcc ggcgactggc
     3241 tgtgggtctg tccgccgaag atgccgccgc catagggcga gcgcaccgtg atcggggccc
     3301 agaactcgcc gttcgaccga tatctgaggc gcgccgcctc cgacaccagt tggtcgaacg
     3361 ccggcaggat gtagtcggcg aactggatct cggggatcgg cctcagaccg tacgcgccca
     3421 tgccgatggc ggtggcgatg atgccgcctt ccgaaatcgg cgcgtcgaag cagcgcgtca
     3481 ggccgtgctt cttctgaagg ccgtccgtca cccgaaagac gccgccgaaa tagccgacgt
     3541 cctcgccgaa gatcagcgtg tccggatcct cggtcagcat cacgtcgagc gccgagttca
     3601 gcgcctggat catgttcatc gtcggcatgg cgtcagatcc caagttcgcg gcgttgttcg
     3661 gtgacccgcc agtcggggtc cttgaagacg ccctcgaaca tctccttgac gctcggcttg
     3721 gacttgccga gcgtgccgac cgcctcggcc tccttgacgg cggcgcgaac ctcggcgtcg
     3781 agctcggcga tcagggcggc gtggcgatcc tcgtcccagg cgtcgagccc gatcagatgc
     3841 gtcttcaacc gctcgaccgg gtcgcccagg ggccaggcct cggcctcgtc cgccggtcgg
     3901 tatttggtcg gatcgtcaga ggtcgaatgg cccgacgctc gataggtgta gagttcgatc
     3961 agggtagcgc cgaggttctg ccgcgcccgc tcggccgccc attccgtcgc ggcccaaatg
     4021 gccaggaagt cgttgccgtc gacccgcagg ccaggcagcc cataggccaa agccttggcc
     4081 gcgaaggtcg tttcgttggc cccggcgatg cccgagaagc tggagatggc ccattggttg
     4141 ttggtcacgc ataggatgac cggcgccttg tagacgctgg cgaaggtcaa ggcctcgtgg
     4201 aaatcgccct cggccgtggt gccttcgccg atataggcca gcgcgatcga atcgccgccc
     4261 ttgaacgccg aggccatggc ccagccgacc gcatggccga agcggctgcc gacgttgccc
     4321 gacagggaat agaagccgta cttccgcgcc gaatacagga tcggcagttg ccgccccttg
     4381 agcggatctt tttcgttgga gaagatctgg ttgcacagat cgatcagcgg atagttgcgc
     4441 gccatcaacc agctgagcac ccgataggtc ggaaagcaca tgtcgtctcg gtccaggaac
     4501 aaggactgcg ccgcgccaat cgcctcctcg ccggtcgact tcatatagaa gctggtcttg
     4561 ccctgacgat gcgcccggaa cagccgctcg tcgaaaaccc gggtcagcaa catggcgcga
     4621 agaccggcca ggagcgtgtt ggtcggaagg ttcggattcc agggcccctt ggcctcgcct
     4681 gaatcgtcga gcacgcggat caggccatag ggcagatcgc gaacctcggc ttcaagagtg
     4741 gacgcctcgg ggcggcgtgt gtcgccgggc tcggggatca gggcgcgatc gaacgccggc
     4801 gtgtcgcccg ggcgcgcgtc gggttctgga atgtgcagac gaaggggagg acgattgttc
     4861 atgatagcgg ccagcgagat gggaggccca atttgccttc tgatatcaga aataaaattg
     4921 tctatttgga acgatatacc gagccatacg cattggctga tgcgcctctg gccaaaattg
     4981 tcgcttcgcg ttgcgcgcta cgccataagg ttggatcagc gcagtcaaag cgcgcatcgc
     5041 cgccctcagg attctttatg gcttctccac ccgatctcga cgaaaccgac ctcaaaatcc
     5101 tcgatgtcgt ccagcgcaac gcgcccatga cgaccgcgga actcgccgac cgcgtgggca
     5161 tgtcgcagtc gccatgctgg cgccgcctca cccgtctgaa ggaggaaggc tacatcgtcg
     5221 accaagtcac ccgcctctcc gcggaaaagc taggcctgcg caccaacgtc ttcgccaacg
     5281 tcaagctttc cgcgcacggc cagagcaatc tcaacgagtt catcgaggcc gtgtcgcgaa
     5341 ttcccgaggt gctggaaatc catccgacga tgggaccgtt cgacttcctg ctgcggatca
     5401 tcacccgcga cgtcgaggac tatcgcgacc tggcgttcgg gaaacttttg acgctgccca
     5461 ccgtccagga aatcaactcc acgatgtcgt tgggccccgt caaaatgaca acggcgcttc
     5521 cgatccgata gcgcctaggc gatccccgtc accggttcca gatcgaggag cgacgggttc
     5581 aaggcgctgt gcgatcgcat cggctcgaac agggcctcga cctgcatcag ggttcaaaca
     5641 tgcgggactc gcgatcgcgg atcatcgggc ttgtcctctt gaagcaggcg gcggttaggt
     5701 cgcgttccgg cccgtgacga tggcgcgggc gatggtcgtg gcgcgggcga tttccgtcag
     5761 accctggcgc gcttccagag cgtggcgcat cgccgacacg gtcggcggca ggatgtcgct
     5821 agtccaggta atctgccgga tggtgaagcc tgcgcggcgc agttggagga ttgtcgtcag
     5881 gcactccaga gcattgtcat caatgatccg gcgaccgccc acgcgtttga actcgacgat
     5941 gccggcctct tcccaatgac gaaccatcgt cgctgtcagg cccaaccgct gcagggcctg
     6001 gctgaaggga gacggcggcc gtgcgaccgg ttcgccgctt ggcttgggac ccgcgcgctt
     6061 ggcccgacgt tcagctcggg cgaacatcgc ttgggccgac ctggtcatcg gtcccgcata
     6121 gtcgctgcga aggatcattc cctgcctcct cttggggttg gaatgccgcc gaggcgctct
     6181 ccagatcgac aagcgcctcg cgcaacgccg ccaaatcgcg ctctttgtgc tcggcagcgt
     6241 cgatgagtgc ttcccggatg gcttgcaggt atcgaggtcg gtcgtggcgc aaggcgatga
     6301 tcgaacgaat tactgggatg gcgatgtcgg ctcttctgag aaaggcgatc acttccagcg
     6361 tggcgacggc ttggctgtcg tagtgcctag tgttgccaaa atccctttcc gaatgaacga
     6421 gcccaagcgc ctcgtagtgg cgcaacgcgc ggtgggagag gccaagacgc gcggccaaga
     6481 cattgatcgg caccaggacg gaggaacgaa gccgtatcgg ccgtgacggg gcaatcgacg
     6541 ggatcatgcg aactccacca agacttcatc ggcggccacc ggatcgccac ccttggcgtt
     6601 gacggccttg accaggccgt cgcgctcggc gcggatgatg ttctgcatct tcatggcctc
     6661 gagcacgcag accacctcgc cttcgcggac ctgctgaccg gccgtgacgt ccatcgacac
     6721 caccaggccc ggcatgggcg acagcaccag cttggaggtg tcggccgcct gcttctcggg
     6781 cagcttgtcg tgcagttcgg ccgagcgcgg ggtcaggacc agcacgcgag ccttggcggc
     6841 gcggtggcgg acggtgaagc cctcggctgc cggagcaacc tggaccgtga agggcttgcc
     6901 gtcgagctcg ccccggaagg tcggcaggcc cggccgccaa tcgatggcag acaacgtcaa
     6961 ggtccggccc tcgtccagca gctcgaccga caaggtcccc tggcccgcca gcttcacccg
     7021 gcgcttggcg tggccgacgg ccaccaccca ttcgtcgcgc accgggccgc cgaagaccac
     7081 cgcgttggag cgagcccggg ccgcatagac ccgttgcatg gccgcgccga caacgatcag
     7141 gatgtcgatc tgagccgggg tgggttcggt gccggtgaag ccgtccggga actcgtcttt
     7201 gatgtagttg gtcgacagct ggcccgagcg gaagcgctcc tggtccatca ccgcgttcag
     7261 gaacgggatg ttctggccca gcccctcgat atggaagtcc tccagcgccc ggcccatgcc
     7321 gtcgaccgcc gcgatgcggg tcggggccca ggtgcagagc ttggagatca tcgggtcgta
     7381 gaacatcgag atctcgtcgc cctcgcgcac cccggcgtcg ttgcgcacaa cgtagccttc
     7441 atgctgtccc tcctccggag gggcatagcg aaccaggcgg ccgatgctgg gcaggaactt
     7501 gcggtagggg tcctcggcgt agatgcggct ctcgatggcc cagccgttga tcttcaggtc
     7561 cttctgcgcg aaggccagct tctcgcccca ggcgctgcgg atcatctgct cgaccaggtc
     7621 caggcccgtg atcagctcgg tgaccggatg ctcgacctgc aggcgggtgt tcatctccag
     7681 gaagaagaag ctcttgtcct ggccggccac gaactcgacc gtgccggcgc tgtcgtagtt
     7741 cacggccttg gccagggcca cggcctgggc gcccatggcg ttgcgggtgg cctcgtcgag
     7801 cagcggcgat ggcgcttcct cgatgacctt ctggttgcga cgctggatcg agcattcgcg
     7861 ctcgaacagg tggacgacgt tgccgtgctt gtcgcccagc acctggatct cgatgtggcg
     7921 cggcgagacg atgaacttct cgatgaagat gcggtcgtcg ccgaagctgg ccttggcctc
     7981 ggcgcggacg gccgggaagc cctcctcgac gtcctggcgg gtccaggcga cgcggatgcc
     8041 cttgccgccg ccgccggccg aagccttgat catcaccgga tagccgatct cctcggagat
     8101 cttcacggca tgggcggtgt cgtcgatctc gccgatatgg ccgggcacgc aggagacgcc
     8161 ggcagcctgg gcgaacttct tgctctcgat cttgtcgccc atggcgcgga tggcgccggg
     8221 gttggggccg atgaaggcga tgccctcgtc ggcgcagcgt tgggcgaagc ccgcgttttc
     8281 cgacaggaag ccaaaacccg ggtgcacggc ctgggcgccg gtctgcttgc aggcgtcgat
     8341 gatcttgtcg gcgacgagat acgactggtt ggccggcgac gggccgatat gcacggcctc
     8401 gtcggccatc tcgacggcca tgctgcccgc gtcggcgtcg gaatagacca ccacggtcgc
     8461 gatccccagc cggcgacacg tcttgatgac gcggacggcg atctcgccac ggttggcgat
     8521 caggatcttg ctgaacattg gcgttgcttc ttggcgggag cggaaaggag ggtgtctgaa
     8581 ccgctgttgc tgatttcgag gttcatgacg atgtcgccat agttgtcggt gacgaggatg
     8641 cgatgatcga tcatcaatat gcggtcgctg atctcatcga cggagacggg tgctggagct
     8701 ccgctgtgtt tccaccagat cccaagacga aacagtcgcc ccgtccgcgc gtcgagcatc
     8761 cctggccaac ggccctcgcc ataaccggcc tgtacccagc caagcgcgtc acgcccggga
     8821 tgatccgtcg cgtcgctcat agcgggatgt tgtcgtgctt cttccacggg ttggagagtt
     8881 ccttgccctt gagggacttg agcccccgca cgatccggcg gc
//
LOCUS       DAOMEO010000009         8538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1311_length_8538_cov_2.536720, whole genome shotgun sequence.
ACCESSION   DAOMEO010000009 DAOMEO010000000
VERSION     DAOMEO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_8538_cov_2.536720"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLH31_00415"
     CDS             <1..257
                     /locus_tag="PLH31_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87750.1"
                     /translation="DGRRGVLTLEAGLEAKDPKLMERAQQSEPRLRAAFAQVLMTYAS
                     GMRRGDPPDVDYLSREMQKTADKVLGKPGSRVLLGSALMN"
     gene            complement(254..754)
                     /gene="tadA"
                     /locus_tag="PLH31_00420"
     CDS             complement(254..754)
                     /gene="tadA"
                     /locus_tag="PLH31_00420"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQR87751.1"
                     /translation="MRTDPPDDQDQWAQDQQAMRLALDAARAAAARGETPVGAVILDP
                     KTGEILGTAGNGPIVAHDPTAHAEIAVMRQVAAKLENYRLTDLTLVVTLEPCAMCAGA
                     ISHARIGRVVFGAEDPKGGAVVHGPRFFAQPTCHWRPEVTGGVMAQESADLLRGFFRA
                     RRKAKP"
     gene            806..2221
                     /locus_tag="PLH31_00425"
     CDS             806..2221
                     /locus_tag="PLH31_00425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQR87752.1"
                     /translation="MTDTPHDPLYDPGHDGDLDDPSTGERVAKALARAGVASRREVER
                     LIEAGRVAINGKVLTTPAVKVGPGDFLTVDGKLVAEREPTRVFRYHKPTGLMTTHNDP
                     KERPTVFQALPRELPRLLSVGRLDLNSEGLLLLTNDGELSRKLEAPNNGWVRRYRVRA
                     FGHTDQARLDRLKDGVTIEGVRYGSIEAKLDKAQDKAGGGKNVWITISLTEGKNREIR
                     RVLEHLDLKVNRLIRLSYGPFALATLMPGQIEEVGPRVIRELLAGLVADEDMPTGDKP
                     QFMGVADPLHAPGTAKGGELQRRAIKSAPLPKVLEPGERTLEKPVYKPGWAKPKKKVV
                     IAGVKPIKRAPKSIETKMIGPKPDHLAPRGAKAATGPKSFGAKAAGGVAGARSFDKAG
                     GKPSSARPEPGKTAGKPFAAKPAGAAGKSFGKPSVKPGVKPSTRPAPKPDQAKPTRAA
                     PAAFEARPGAPRPYKGPKKPR"
     gene            complement(2218..3183)
                     /locus_tag="PLH31_00430"
     CDS             complement(2218..3183)
                     /locus_tag="PLH31_00430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQR87753.1"
                     /translation="MAAIFEAMRDSRFFADLTAYDREPRRLLVAVPVGLLAAAAAAVL
                     GAASACLMVMVIVSGLDGAPAAAALFSVFDQPARAETDGQASLFILSVLAGVNGAAAL
                     AFVAVGAGISHRPIRRYVTVETAFRTRLLVAGLVLVGAIMAALILISHAVSAGMPTPP
                     VLTLAATTFDRATYAAFAIVLLVIAAAAEEIIFRGWLLKHAGAFTRNPAILMAVNGLL
                     FALIHFDPNPGAFLVRAAMGAGLTWMTLRTGGIELAIGAHAANNIAILLLLRPLSLTP
                     EPQQPFQLTSLVTVAALGVGYVLLAEAVVRWPIVTRWFGGRPATV"
     gene            3318..3878
                     /gene="rsmD"
                     /locus_tag="PLH31_00435"
     CDS             3318..3878
                     /gene="rsmD"
                     /locus_tag="PLH31_00435"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQR87754.1"
                     /translation="MRIVSGQYRGKAIVAPPGDATRPTSDRARQAVFNILEHAAWAPE
                     LHGARVVDLFAGSGALGLEALSRGAAFCLFVETADPARGAIRENIDAMGLFGVTRVHR
                     RDATDLGLPPSSVGAPFDIAFLDPPYEKALAQLLAHGWLKPGAIVMFERGREEPEPAT
                     PGFTVLDTRDYGAARVLFLKATSPAP"
     gene            complement(3859..4134)
                     /locus_tag="PLH31_00440"
     CDS             complement(3859..4134)
                     /locus_tag="PLH31_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HQR87755.1"
                     /translation="MNISKAQQRTLHALAQGARIEPVRDDHGRLVDVDCITREGWRLS
                     DCNLGVFKALKKRRLIASSGGGPYRITREGAVSLRSQLDNRVTARGW"
     gene            complement(4461..6563)
                     /locus_tag="PLH31_00445"
     CDS             complement(4461..6563)
                     /locus_tag="PLH31_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQR87756.1"
                     /translation="MQGQPEIQALSDHEHIAPKRDPLERLRARWALMPTSARRGAIAA
                     VALFVAVIAFLIIFDWNWLRGPIGRIASAQLDRKVEIVGDLRVHPWSFSPKVEALDLR
                     IGQPDWALKADPTLPPMARVQRLAVQFKLLPLFKGDVILPLLAIDRPQVRLIRDASGQ
                     ANWTFGAKKANAKPLKLPAIQHFIINEGQLRVDDRQRDVLFEGAVSSNEQASGDGHGK
                     FVLEGKGRLNRSPFTAMVTGGPLLNITPNRPYPFDARVVAASTRVTAKGSVTKPFDLG
                     RFVADITVSGTDLNRLYALTGLTLPNTPPYQISGKLTRKGGRFDFNGLSGKIGDSDIS
                     GDLFVLTQRERPYLEAKLQSRRLDFDDLGSLVGAAPATGRGETASAGQKVEASQREAT
                     QRLLPDATLQTERVRAMDAKVQYRALAVNAPGFPLKKVRLDLTLDKGVLEMDPIAFTF
                     SHGDLSGKVRLDARPDVPRTDLDLRLTNARLQDFIPVQSGGKPIIEGPVMARAKLSGV
                     GNSIHRAASSANGTFTMVSPRGTIRQAFAELMGVNLSKGVLMLLAKDTDETAVRCAVA
                     DFTVKNGVATTNHLVADTGVVLVRGKGQINLKTERLDFRIDGDSKKPRLLRLFVPITI
                     SGPFLTPKVGFKATAAVSQGGVATALGVLVNPLAALLPFITTGEAKNADCQGLVADAR
                     GEGVPVKVGQTTAAPVKK"
     gene            complement(6624..6728)
                     /locus_tag="PLH31_00450"
     CDS             complement(6624..6728)
                     /locus_tag="PLH31_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87757.1"
                     /translation="MRTPTKPFFKRYELIAIAAFVVAAIGVSSLGLVY"
     gene            complement(6804..7334)
                     /locus_tag="PLH31_00455"
     CDS             complement(6804..7334)
                     /locus_tag="PLH31_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87758.1"
                     /translation="MTQSRPLPRLAVLVAAAGVLFTTAAMASDHGLRGGPPVSRGGLP
                     LDPELLPPNTGPGDCVTRRVTGPGGAYRWDRVECEPERGWTGFDPWGYGRRPLEVETL
                     PSHNADRYSGRRAEEIDGRRRERRFDAYAGSGDNDSRAPNRPEHRYGPGPSRAYAYRV
                     AGRDADGFLVWPGKQP"
     gene            7469..8110
                     /locus_tag="PLH31_00460"
     CDS             7469..8110
                     /locus_tag="PLH31_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQR87759.1"
                     /translation="MAIEAVIWDFGGVFTTSPFEAFRRFEAERGLPADFIRTVNSTDP
                     DSNAWAKFERSEIDAAGFDTLFLEEATRLGHAVRGADVLPLLYGDLRPRVVSALLACK
                     AKFKVGCITNNVTTGHGAGMASSAEKAAAVAQIMSHFAVVIESSKAGVRKPDPRIYLM
                     MCEALEVAPENCVYLDDLGINCKPAAALGMTAIKVSNEDQLLADLAKATGLAF"
     gene            8137..8418
                     /locus_tag="PLH31_00465"
     CDS             8137..8418
                     /locus_tag="PLH31_00465"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQR87760.1"
                     /translation="MSRPKLGAAAVTAQLSGWSAAPDKDAITKTFKFADFNEAFGFMT
                     RVALMADKLDHHPEWFNVYNRVEVLLTTHDADGVTELDLALATFMDSVA"
BASE COUNT         1408 a         2897 c         2957 g         1276 t
ORIGIN      
        1 gcgacggtcg gcgcggcgtg ctgaccctgg aggccggcct cgaggccaag gatcccaagc
       61 tgatggagcg cgcccagcag tcggaacccc gcctgcgcgc cgccttcgcc caggtgctga
      121 tgacctatgc ctcaggcatg cgtcgcggcg atccgccgga cgtcgactac ctgtcgcgcg
      181 aaatgcagaa gaccgccgac aaggtgctgg gcaagcccgg cagccgggtg ctgctgggtt
      241 cggcgttgat gaactagggc ttggccttcc gccgcgcgcg gaagaaccct cgcagcaggt
      301 cggcgctctc ctgcgccatg accccgcccg tcacctccgg ccgccagtgg cacgtgggct
      361 gagcaaagaa gcgcgggcca tggaccaccg cgccgccttt ggggtcttcg gccccaaaca
      421 ccacccgccc gatccgtgca tggctgatgg ccccggcgca catggcgcag ggctccaggg
      481 tcaccaccag ggtcaggtcg gtgagccgat agttttccag cttggccgcc acctgccgca
      541 tcaccgcgat ctcggcgtgg gcggtagggt cgtgggccac gatcggacca ttgcccgccg
      601 ttccgagaat ttcgccggtc ttcgggtcaa ggatcacggc gcccaccggc gtctcgcccc
      661 gcgcggcggc ggcgcgggcc gcgtccagcg ccaggcgcat ggcctgctgg tcttgggccc
      721 actggtcttg atcgtccgga ggatcggtgc gcatttgccc aaaagcccat cgtgagatgc
      781 gcccagcccc tcgaaggaac gagatatgac cgatacgccc cacgaccccc tatacgatcc
      841 cggccacgac ggcgacctcg acgatccgtc gaccggcgaa cgcgtcgcca aggccctggc
      901 ccgggcgggc gtggcctcgc ggcgcgaggt cgaacggttg atcgaggccg gccgtgtggc
      961 catcaatggc aaggtcctga ccacccccgc cgtgaaggtg gggcccggcg acttcctgac
     1021 ggtcgacggc aagctggtgg ccgagcgcga gcccacccgg gtgttccgtt atcacaagcc
     1081 caccggcctg atgaccaccc acaacgaccc caaggagcgc ccgacggtgt tccaggccct
     1141 gccgcgcgag ctgccgcgcc tgctctcggt ggggcgactg gacctcaact ccgaaggcct
     1201 gctgctgctg accaatgacg gcgagctgtc gcgcaagctg gaagccccca acaacggctg
     1261 ggtgcgccgc tatcgcgtcc gggcctttgg tcacaccgat caggcgcgtc tggaccgact
     1321 gaaggacggc gtcaccatcg agggcgtccg ctatggctcg atcgaggcca agctcgacaa
     1381 ggcccaggac aaggccggcg gcggcaagaa cgtctggatc accatctcgc tgaccgaggg
     1441 caagaaccgc gaaatccgcc gggtgcttga gcacctggac ctgaaggtca atcgcctgat
     1501 ccgcctgtcc tacggcccgt tcgccctggc caccctgatg ccgggccaga tcgaggaggt
     1561 cggtccccgg gtgatccgtg agctgctggc cggcctggtg gcggacgaag acatgcccac
     1621 cggcgacaag ccgcagttca tgggcgtggc cgatccgctg cacgcgccgg gcaccgccaa
     1681 gggcggcgag ctgcagcgcc gggccatcaa gtcggcgcct ctgcccaagg tgctggagcc
     1741 gggcgaacgc acgctggaaa agccggtcta caagcccggc tgggccaagc cgaaaaagaa
     1801 ggtggtgatc gccggcgtca agccgatcaa gcgtgcgccc aagtcgatcg agaccaagat
     1861 gattggcccc aaacccgacc acttggcgcc gcgcggcgcc aaggcggcga cgggtccaaa
     1921 gtcgttcggg gcgaaggcgg cgggcggcgt ggccggcgcc agaagcttcg acaaggccgg
     1981 cggcaagccg tcctcggcga gaccggaacc tggcaagacg gccggaaagc cgttcgccgc
     2041 caagcccgcg ggcgcggcgg gcaagtcgtt tggcaagccg agcgtcaagc cgggcgtcaa
     2101 gccttccacc aggcccgccc caaagcccga ccaagccaag ccgactcgcg cggcgccggc
     2161 ggctttcgaa gcaaggccgg gcgcgccgcg tccctataag ggccccaaga agccgcgcta
     2221 aaccgtggcg ggccgaccgc cgaaccagcg ggtgacaatg ggccagcgca ccacggcctc
     2281 ggccagcagg acgtagccga ccccgagcgc cgcgacggtg accagcgagg tcaactggaa
     2341 gggttgctgc ggttccgggg tcagcgacag cggccgcagc agcagcagga tggcgatgtt
     2401 gttggcggcg tgggcgccga tggccagctc gatcccgccc gtgcgcagcg tcatccaggt
     2461 caggcccgcg cccatggccg cccgcaccag gaaggcgccc ggattggggt cgaagtggat
     2521 cagggcgaac agcaggccgt tcaccgccat caggatcgcc ggattgcggg tgaaggcgcc
     2581 cgcatgtttc aggagccagc cgcgaaagat gatctcctcg gcggcggcgg cgatcaccag
     2641 cagcacgatg gcgaaggcgg cataggtcgc gcggtcgaag gtggtcgccg ccagggtcag
     2701 gaccggcggc gtgggcattc cagcgctgac agcgtgcgag atcaggatca gagcggccat
     2761 gatcgccccc accagaacca ggcccgcgac cagcagccgc gtgcggaagg cggtctcgac
     2821 cgtgacatag cgccggatcg gacgatggct gatcccggcg cccaccgcca caaaggccag
     2881 ggccgccgcg ccgttgacgc ccgccagcac cgacaggatg aacagcgagg cctgaccgtc
     2941 ggtctcggcc cgggccggct ggtcgaacac cgaaaacagc gcggcggcgg ccggcgcgcc
     3001 gtccaggccg ctgacgatca ccatcaccat caggcaggcc gatgcggcgc ccagcacggc
     3061 ggcggcggcg gcggccagca ggcccaccgg cacggcgacc agcaatcgcc gcggctcgcg
     3121 atcataggct gtcaggtccg cgaagaagcg cgagtcccgc atcgcctcga agatcgccgc
     3181 catcgccatc gcgttttctg acacccttgt tcttcgggcg actaagagag ggctcgccca
     3241 aacttgtaaa gcgtccggct tgccgtggcc gctcaacagc ggcacacacg ccggccaaga
     3301 cagggatttg cagctcgatg cgcatcgttt ccggccagta tcgcggcaag gccatcgtcg
     3361 ccccgcccgg cgacgccacc cggccaacct ccgaccgcgc ccggcaggcg gtgttcaaca
     3421 tcctggagca cgccgcctgg gcgccggagt tgcacggcgc gcgggtggtc gacctgttcg
     3481 ccggctcggg cgccctggga ctggaggccc tgtcgcgcgg ggcggcgttc tgcctgttcg
     3541 tcgagaccgc cgatccggcg cgcggcgcga tccgcgaaaa catcgacgcc atgggcctgt
     3601 ttggcgtcac ccgcgtgcat cgccgcgacg ccaccgatct tggcctgccg ccgtccagcg
     3661 ttggcgcgcc gttcgacatc gccttcctgg atccgcccta cgagaaggcc ctggcccagc
     3721 tgctggctca cggctggctc aagcccggcg cgatcgtgat gttcgagcgc ggccgcgagg
     3781 agcccgagcc cgccacgccc ggcttcacgg tgttggacac gcgcgactat ggcgccgcgc
     3841 gtgtcctgtt cctgaaggct accagccccg cgccgtgacg cggttgtcga gctgcgacct
     3901 caggctgacc gcgccctccc gcgtgatgcg gtaggggccg ccgccgctgc tggcgatcag
     3961 ccgccgcttc ttcagcgcct tgaagacgcc gagattgcag tcgctgaggc gccagccctc
     4021 gcgggtgatg cagtcgacgt cgacgaggcg gccgtggtcg tcgcgcacgg gttcgatgcg
     4081 ggcgccctgc gccagcgcgt gcagcgtgcg ttgctgcgct ttggagatgt tcaaggaaat
     4141 atccggggat cgaggcgtag gaacgcgcgt ccagcgggac acgcggacgg atcagggcct
     4201 gatcgacttg cgaaccagag gttcggagcc tgttaggccc gggcctcgaa tacaatctcg
     4261 gacataaaac caccgttggc gtgatggggg tttatagcgc ggctggaggc gcgccctcaa
     4321 gcggctgcgg ttcgttgccc ataaactgtg tcatcctggc cgcagcgcag cggagagccg
     4381 ggaccgccgg aggcgctgag ttcgggcgtc ggtcccggat cggcgcgcta cgcgcttgtc
     4441 cgggatgaca atgcttgtag ctatttcttc accggcgccg ccgtggtctg acccaccttc
     4501 acaggcacgc cctcgccgcg cgcatcggcc accaacccct ggcagtcggc gttcttggct
     4561 tcgccggtgg tgatgaaggg cagcagggcc gccagcggat tgaccagcac ccccaaggcc
     4621 gtcgcgacgc cgccctggct gacggccgcc gtcgccttga acccgacctt gggcgtcagg
     4681 aatggtccgg aaatggtgat cggcacgaac aggcgcagca ggcgtggctt cttgctgtca
     4741 ccatcgatgc ggaagtccag ccgctcggtc ttcagattga tctgaccctt gccgcgcacc
     4801 agcaccacac cggtatcggc cacaagatgg ttggtggtgg ccacgccgtt cttcaccgtg
     4861 aagtcggcca ccgcgcagcg cacggcggtc tcgtcggtgt ccttggccag cagcatcagc
     4921 acgcccttgg acaggttgac gcccatcagc tcggcgaagg cctggcggat cgtgccgcgc
     4981 ggcgagacca tggtgaaggt cccattggcg ctggaagcgg cgcggtggat gctgtttccc
     5041 acgcccgaaa gcttggcgcg cgccatcacc ggcccttcga tgatcggctt gccgccgctc
     5101 tgcacgggga tgaagtcctg cagacgcgca ttggtcaggc gcagatccag atcggtgcgc
     5161 ggcacatcgg gccgggcgtc cagccgcacc tttcccgaca ggtcgccatg cgaaaaggtg
     5221 aaggcgatgg ggtccatctc cagcacgccc ttgtcgagcg tcaggtccag ccggaccttc
     5281 ttgagcggaa agcctggggc gttcacggcc agggcccgat actgcacctt ggcgtccatg
     5341 gcccgcacgc gctcggtctg cagggtggcg tcgggcagca ggcgctgggt cgcctcgcgc
     5401 tggctggcct cgaccttctg tccggccgag gcggtctcgc cgcgaccggt ggccggcgcc
     5461 gcgccgacca ggctgcccag atcgtcgaaa tccaggcggc gcgactgaag cttggcctcc
     5521 agataaggcc gctcgcgctg ggtcagcaca aagagatcgc ccgagatgtc gctgtcgccg
     5581 atcttgcccg acaggccgtt gaaatcgaag cgaccgccct tgcgggtcag cttgccgctg
     5641 atctgatagg gcggcgtatt gggcagggtc aggccggtca gggcatagag gcggttcagg
     5701 tccgtgcccg acaccgtgat gtcggcgaca aaccgcccca ggtcgaacgg cttggtcacc
     5761 gagcccttgg ccgtgacgcg ggtggaggcg gccaccaccc tggcgtcgaa cggatagggc
     5821 cggttgggcg tgatgttcag cagcgggccg ccggtgacca tggcggtgaa cggcgagcgg
     5881 ttgaggcggc ccttgccctc caggacgaac ttgccgtgac catcgccgct ggcctgctcg
     5941 ttggagctga ccgcgccctc gaacagcaca tcgcgctggc ggtcatcgac ccgcaactgg
     6001 ccctcattga tgatgaagtg ctggatggcg ggcagcttca gcggcttggc attggccttc
     6061 ttggcgccga aggtccagtt ggcctggccg gacgcgtcgc ggatcaggcg cacctgcggc
     6121 ctgtcgatcg ccagcagcgg caggatcacg tcgcccttga acagcggcaa cagcttgaac
     6181 tgcaccgcca gccgctggac cctggccatc ggcggcaggg tcggatcggc cttcagcgcc
     6241 cagtccggct gaccgatacg caggtccagc gcttcgacct tgggcgagaa cgaccaggga
     6301 tggacgcgca gatcgccgac gatctcgacc ttgcggtcca gctgggccga ggcgatgcgg
     6361 ccgatgggac cgcgcagcca gttccagtcg aagatgatca ggaacgcgat caccgcgaca
     6421 aacagcgcca cggccgcaat cgcaccgcgt cgcgccgagg tcggcatcag cgcccagcgg
     6481 gcgcgcagcc gctcaagcgg atcgcgtttt ggcgcgatgt gttcgtgatc ggacagggcc
     6541 tggatctccg gttgcccctg cacaacgcgc gggctgggcg atcctgttcc attccggcgc
     6601 caagacgcct ggccggaagc cttttagtag acgaggccca ggctgctgac gccgatcgcg
     6661 gccaccacga aggcggcgat ggcgatcagt tcgtagcgct tgaagaaagg cttggttggc
     6721 gtacgcatgg tttcaggtcc cccgtcccaa attggttcgt cgggaaggac ggcccgcaag
     6781 gctcgggcca gtcaggccaa ggatcagggt tgttttccgg gccagacgag aaagccgtcg
     6841 gcgtctcggc cagccacccg gtacgcgtag gcgcgcgacg ggccagggcc gtagcggtgc
     6901 tcgggccggt tgggagcgcg ggagtcgttg tcgccagatc cggcgtacgc atcgaaccgg
     6961 cgctcccggc gtcgcccgtc gatctcctcg gcgcgacgtc cgctgtagcg gtcggcatta
     7021 tgcgaaggca gggtctcgac ctcgagcggg cggcggccat agccccacgg gtcaaagccg
     7081 gtccagccgc gttcgggctc gcactcgacg cggtcccagc gataggcgcc gccggggccg
     7141 gtgacgcggc gcgtcacgca gtcgccgggg ccggtattgg gcggtagcag ttccggatcc
     7201 agcggcaagc cgccgcgcga aacgggcggg ccgcccctca gaccgtggtc ggaggccatg
     7261 gcggcggtgg tgaagaggac gccagcagcg gcgacgagaa cggcgaggcg cggaagcggg
     7321 cgagactggg tcatggcgac aagaaaccat taacgaagat gaacggcgct agggttcacc
     7381 cgacgcgcga acggagcttg agcttggcgg cgcttgcctc gtcttgggcc tcgccctacc
     7441 gtggatcaaa caactgtttg agggcggcat ggcgatcgag gcggtgatct gggatttcgg
     7501 cggcgtgttc accacctcgc cgttcgaggc cttccgacgc tttgaggccg agcgcggcct
     7561 gccggccgac ttcatccgca cggtcaactc caccgatccc gacagcaatg cctgggcgaa
     7621 gttcgagcgt tcggagatcg acgcggccgg gttcgacacg ctgttcctgg aagaggccac
     7681 ccgcctgggc cacgcggtgc gcggcgccga cgtattaccg ctgctctatg gcgacctgcg
     7741 gcctcgggtg gtgtcggcgc tactggcctg caaggcgaag ttcaaggtcg gctgcatcac
     7801 caacaatgtc accaccggcc acggggcggg catggcctcc agcgccgaaa aggccgcggc
     7861 ggtcgcgcag atcatgagcc atttcgcggt ggtgatcgaa agctccaagg ccggcgtccg
     7921 caagcccgat ccgcgcatct atctgatgat gtgcgaggcc ctggaggtgg cgcccgagaa
     7981 ctgcgtctat ctcgacgacc tggggatcaa ctgcaaaccg gctgcggccc tgggcatgac
     8041 ggcgatcaag gtctcgaatg aggaccagct gctcgccgac cttgcgaagg cgaccggttt
     8101 ggcgttctga agggagacag tcctccagga gccgccatgt cccgccccaa gctcggcgcc
     8161 gccgccgtca ccgcacagct gtccggctgg agcgccgcgc ccgacaagga tgccatcacc
     8221 aagacgttca agttcgccga cttcaatgag gcttttggct tcatgacccg cgtggccctg
     8281 atggccgaca agctcgacca ccatcccgaa tggttcaatg tctacaaccg ggtcgaggta
     8341 ctgctgacca cgcatgacgc cgatggggtg actgagctgg atctggcgtt ggcgacgttc
     8401 atggattcgg tggcttagcg gccgacgtcg ggacgccccc tccgtcacgc tgcgtattcg
     8461 cagcgcgcca cctcccccgc aagcggggca ggagggtgat gctgttcttc tcctgccccg
     8521 tagcgaagcg tgcggggg
//
LOCUS       DAOMEO010000010         8265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1346_length_8265_cov_2.786236, whole genome shotgun sequence.
ACCESSION   DAOMEO010000010 DAOMEO010000000
VERSION     DAOMEO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_8265_cov_2.786236"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="PLH31_00470"
     CDS             complement(<1..1584)
                     /locus_tag="PLH31_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87761.1"
                     /translation="MKTTLFAGASVGLIILASATAASAQSIDYGAMEQLFNEPVTTSA
                     TGSPQRSTEAPVAMEIISAADIKRSGATDLPTIISRVAGLDVLNAGAAGADVSVRGYV
                     MSMSPRLLVLINGRQVYLDHYGYTAWATLPVQLEEIRQIEVVKGPNSALFGFNAVGGV
                     VNIITFNPKFDNVGSVTVRAGVGGHQEISAVKTVKLGDKFSARLSAGAVQMDEWKNTA
                     GIANNLLTDPARVSANLDTLTQLNDKTELRIEGSWSNVQISEVVSNNSYSPTKYVTSS
                     VKGALASETKFGTLELSAYRNMLSAKHLVVGQAAKFDNEITVVSAQNLFKIGAKHTFR
                     VGAEYRHNEVNTAPIAGGQLSYDVYAASGMWTWAVTDKLSTTAAVRFDKLELERSGTF
                     PAGYPVVTNAYWDRSIEEASYNLGLVYRPTGFDSIRATVAKGVQVPTLVDLGAVQLKV
                     VQGPFTIGVIGNPTLQPSIVTNFEIGYDRALPALNAKIGASVFVQKTEDVKGQPSTTQ
                     LDIAPTATSLPIISYRNIGD"
     gene            complement(1611..2141)
                     /locus_tag="PLH31_00475"
     CDS             complement(1611..2141)
                     /locus_tag="PLH31_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87762.1"
                     /translation="MSNKTKPGALALCALALAAAPLSAHADAAKDLQVAGRALTFLEN
                     GPTGKATLGVVFDPSKPASVAEKNAVMAAIGGGMSAGSLTIAGKAVEAGDVGSVSGVQ
                     ALYITTGVNAGPVGAAAKGKKLITIGSDVSCATSGACVMSVSADPKVEIVVSRSAAAA
                     VGAVFKAAFRMMIREV"
     assembly_gap    2241..2250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2683..3264)
                     /locus_tag="PLH31_00480"
     CDS             complement(2683..3264)
                     /locus_tag="PLH31_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQR87763.1"
                     /translation="MTIVRRNLNWLFVLLASVLVFGALSAAPAHAQQPPPAAAQDGDY
                     LLGSSDKVRVTVYGEPTLSGEFFVTGSGMVSLPLIGEVKAAGLSLRQFQDAVQQSLSD
                     GYLKEPRVSAEVLTFRPFYILGEVTKPGTYPYTSGLTVMNAVATAGGYTYRADKKQVF
                     VKRNGATEEQKMELTPSVTVAPGDTIRIGERFF"
     gene            complement(3341..4132)
                     /locus_tag="PLH31_00485"
     CDS             complement(3341..4132)
                     /locus_tag="PLH31_00485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQR87764.1"
                     /translation="MKFKFFRPKPSPTASASTGGRVVYAVGDIHGHLDLLEDLLGVIT
                     RDFAALNRQDQPVLVFVGDYVDRGPASRGVIDRLIALTTAAAERGVFEVRALMGNHEQ
                     TMLSFLDNPEAGAAWVEFGGGETMTSYGVVRPVGRVDQDAWREIQSHFQARVPASHVA
                     FLQGLELSATYGDYFFTHAGVRPGVPLDRQTPEDLMWIRNDFLSQPHRLPCVVVHGHT
                     PDEEPYIGRDRINIDTGAYATGVLTAVRLIDGPPVILQARKSRRV"
     gene            4441..5220
                     /locus_tag="PLH31_00490"
     CDS             4441..5220
                     /locus_tag="PLH31_00490"
                     /inference="COORDINATES: protein motif:HMM:NF017815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HQR87765.1"
                     /translation="MLAVTTTAHAGARPFMARGETVEAPPGFTDLCQRDETTCGSPAA
                     MVAPTSALAMTALSTASLGRTWPMAMAAAERQASDAAAEATALGLTVVESLQAYRPEA
                     VEDFLPAGAVRQAAPAPTVMAATASPGLLNKSQLKLITTINRDVNRDIRKANDFDIYG
                     MPEFWSLPRVIDGKMYGDCEDYALEKRRRLIEAGVPVEALSMAVAVTLRGERHAVLVV
                     AFEGGDMVLDNLTPWPTPWGELNYTWIQRQIAGSAAWTTIS"
     gene            complement(5340..5501)
                     /locus_tag="PLH31_00495"
     CDS             complement(5340..5501)
                     /locus_tag="PLH31_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87766.1"
                     /translation="MSMIPADLSVTPSAAASGFAAVRRLPRAVGWLSAAVVSAGLWSL
                     AVEAIRLIV"
     gene            complement(5636..6741)
                     /locus_tag="PLH31_00500"
     CDS             complement(join(5636..6480,6480..6741))
                     /locus_tag="PLH31_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611445.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQR87767.1"
                     /translation="MKRSKFSEAQVAFILRQVDEGATVGEVCRKAGISEATFYVWRKK
                     YAGLMPSEMKRLRQLEDENARLKRIVADLSLDKEMLQDVIKRKPLGPARRRQLVDYLR
                     AAWQVSIRRACGALQAERSSYHYKGKRTDPVALKKRIKEIAETRVRYGYRRITVLLKR
                     EGWPVNGKKIYRLYKELGLQLRNKTPKRKVKAKLREDRQPASRPNDVWAMDFLHDQLF
                     DGRKIRILAVIDTFSRFSPALDPRFAYKAHDVVETLDRAGKVVGYPKTIRVDNGPEFV
                     SRDLDLWAYQRGVTLDFSRPGKPTDNAFAESFNGKVRAECLNTAWFMSLDDARSKCEA
                     WRRDYNEHRPHSAIGDKCPIELMNGAGPYGPPGA"
     gene            complement(6820..7479)
                     /locus_tag="PLH31_00505"
     CDS             complement(6820..7479)
                     /locus_tag="PLH31_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HQR87768.1"
                     /translation="MATADPAALDQFISAFTGQVGSGFGAIAGPVQGVLATLVVISIT
                     ISAILWAIDETQNIMAALVRKILLIGFFAWLVTNWHSLTLTVINGFAKLGLQASGGGS
                     IGDFTRAPTKAVEAGLSVVIDLIRYIGDLAQQNAGFGALMHIDMIIVAAVAAIGILLA
                     FIILAIEIAVTIIEFYIVTLIAFVVVPFGVLSKTSFLAEKAIGYVVSVGFKFMALAVI
                     A"
     gene            complement(7707..8189)
                     /locus_tag="PLH31_00510"
     CDS             complement(7707..8189)
                     /locus_tag="PLH31_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87769.1"
                     /translation="MKDLNALMQQAQAIGYNSQNIAGQFQQFYPASLSGQSFAQIQQR
                     LASWEANSRLTLQDSMAVQNQLVRSQGTTAGAVNGAVAASQGAAGQTAAIQATNQLLA
                     ALSSQLQGLQTILIAQARTQETILAQQQAGAIAARAETERATAYTKTGSRLPNISDFQ
                     "
BASE COUNT         1515 a         2575 c         2647 g         1518 t
ORIGIN      
        1 gtcgccgata ttgcgatagc tgatgatcgg caggctggtg gcggtggggg cgatgtccag
       61 ctgcgtggtc gacggctggc ccttcacatc ctcggtcttc tgcacgaaca ccgaagcgcc
      121 gatcttggcg ttcagggccg gcagggcgcg gtcgtagccg atctcgaaat tggtgacgat
      181 cgacggctgc agggtggggt tgccgatgac gccgatggtg aacgggccct ggacaacctt
      241 cagctgcacg gcgccgagat cgaccagggt cggaacctgg acgcccttgg cgacagtggc
      301 gcggatcgag tcaaagccag tcggacgata gacgagaccc agattgtagc tggcctcctc
      361 gatcgagcga tcccagtaag cgttggtcac aaccgggtag ccggccggga acgtgcccga
      421 acgctccagc tccagcttgt cgaagcgaac ggcggcggtc gtcgacagct tgtcggtgac
      481 ggcccaggtc cacatgcccg aggcggcata gacgtcatac gagagctggc cgccggcgat
      541 cggggcggtg ttgacctcgt tgtgacgata ttcggcgcca acgcggaagg tgtgcttggc
      601 cccgatcttg aacaggttct gggcgctgac cacggtgatt tcgttgtcga acttggccgc
      661 ctggccgacg accaggtgct tggccgacag catgttgcga taggccgaca gctccagcgt
      721 gccgaacttg gtttcagacg ccagggcgcc cttcaccgag ctggtcacat atttggtcgg
      781 cgaatagctg ttgttggaca ccacttcgct gatctggacg ttcgaccacg agccttcgat
      841 acgcagctcg gtcttgtcgt tcagctgggt cagggtgtcc agattggccg agacacgggc
      901 cggatcggtc agcaggttgt tggcgatacc ggcggtgttc ttccactcgt ccatctggac
      961 ggcgccggcc gacaggcgag ccgagaactt gtcgcccagc ttcacggtct tgacggccga
     1021 gatctcctgg tgaccgccaa cgcccgcgcg gacggtgacc gagccgacat tgtcaaactt
     1081 cgggttgaag gtgatgatgt tgaccacgcc gcccacggcg ttgaagccga acagggcgct
     1141 gttggggccc ttgacgactt cgatctggcg gatctcttcc agctgcaccg gcagggtcgc
     1201 ccaggcggtg tagccatagt ggtccaggta gacctggcgg ccgttgatca ggaccagcag
     1261 gcgcggcgac atcgacatga catagccacg cacgctgacg tcggcgccgg cggcgccggc
     1321 gttcagaaca tccaggccgg caacgcggct gattatggtg ggcaggtcgg tggcgcccga
     1381 gcgcttgatg tcagcggcgg agatgatctc catcgcgacc ggcgcttcgg tcgagcgctg
     1441 cggcgaaccc gtggcgctgg tggtgacggg ttcgttgaac agctgctcca tcgcgccgta
     1501 gtcgatcgac tgggcggaag cggcggtggc cgaagccagg atgatcaggc cgactgaagc
     1561 gccggcgaag agagtcgttt tcatgggttt gtctctttcg aacgctgggg tcagacttcg
     1621 cggatcatca tgcggaaggc ggccttgaag accgcgccga ccgcggcggc cgccgagcgg
     1681 ctgaccacga tttccacctt tggatcggcc gagacgctca tcacgcaggc gccgctggtg
     1741 gcgcaggaaa cgtcagagcc gatggtgatc agcttcttgc ccttggcggc ggcgccaacc
     1801 gggccggcat tgacgcccgt ggtgatgtag agcgcctgga cgccggaaac gctgccgaca
     1861 tcgcccgctt ccacggcctt gcccgcgatg gtcagcgagc cggcgctcat gccgccgccg
     1921 atggcggcca tgacggcgtt cttctcggcc acggaggccg gcttggacgg atcgaaaacg
     1981 acgcccaggg tggctttgcc ggtcgggccg ttttcaagga aggtgagcgc gcggcctgcg
     2041 acctgcagat ccttggcggc gtcggcgtgg gcgctcagcg gcgcggccgc cagcgccagg
     2101 gcgcaaagcg caagagcgcc cggcttggtc ttgttcgaca ttctgcttat cccctgcccg
     2161 gttgctgccg ggcttgctgc aggggagaaa gacccggagc agtaaacaga aagtaactga
     2221 gttccatcga acgccgttac nnnnnnnnnn aattgtcagt aaaggggccg gattaacgta
     2281 cccgatatgg gtttgatacc gcatatcgca tgaactgtat caaaaatacg gcgattttat
     2341 ctttaaatgg catgaataca tgcctagctg tatttgataa gtctcggcct ggatgcgggg
     2401 ccgaccccga ggtcaggcaa atttcagcaa ccccggcgcc ggccaaaccg aaaagccggt
     2461 ctttactacc gttcaatagc tgaaatgggc attttgtcgc attggcgaac aaaacgtcaa
     2521 aaagggtgtg ttttcgggcg gttggacgtc gaacatggcg tcctagggcc ggccttccgc
     2581 tgcccggggc gcggctcagg cgggctgttc aagcgcaaag cccaccggcg ctacgcttga
     2641 acctgcacct gaaagcgcat tcgtgggcgc ggcgaggcgc gcctagaaga agcgctcgcc
     2701 gatccggatg gtgtcgcccg gcgcgacggt caccgacggc gtcagctcca tcttctgttc
     2761 ctcggtcgcg ccgttgcgct tgacgaagac ctgcttcttg tcggcgcgat aggtgtagcc
     2821 gccggccgtg gcgaccgcgt tcatcaccgt caggccggag gtatagggat aggtgcccgg
     2881 cttggtcact tcgcccagga tatagaacgg ccggaaggtc agcacctcgg cgctgacgcg
     2941 cggctccttc agatagccgt cgctgagcga ctgctgcacg gcgtcctgga actggcgcag
     3001 gctcaggccg gcggccttga cctctccgat caagggcagg gagaccatcc cgctgcccgt
     3061 cacgaagaac tcgcccgaca gcgtcggctc gccgtaaacg gtgacccgca ccttgtcgct
     3121 ggaacccagc agatagtcgc catcctgagc ggccgcgggc ggcggttgtt gggcgtgcgc
     3181 tggcgcggcg ctcagcgccc cgaagacaag cacactcgcc aacaggacaa agagccagtt
     3241 taggtttcgt ctgacgatag tcatcggatc aaagtctctg aataagcgtg tccctgccgc
     3301 gtgtcatgac acgggcgcag agcagcgaca agcacgtcac ttaaacgcga cgagactttc
     3361 gggcctgaag aatgacaggc gggccgtcaa tcaagcgaac ggcggtaaga acgcccgtcg
     3421 cataggctcc ggtgtcaata ttaatacgat cgcgaccgat atagggctcc tcgtcaggcg
     3481 tatggccgtg aacaacgaca cagggcagcc gatgcggctg cgacaggaaa tcgttgcgga
     3541 tccacatcag atcctcgggc gtctggcggt ccagcggcac gccgggcctg acgccggcgt
     3601 gggtgaagaa atagtcgcca taggtggctg acagctccag cccctggagg aaggcgacat
     3661 ggctggccgg cacccgggcc tggaaatggc tctggatctc gcgccaggcg tcctggtcca
     3721 cccggccgac gggacgaacc acgccatagg aggtcatggt ctcgccgccg ccgaactcga
     3781 cccaggccgc gccggcctcg gggttgtcga ggaacgagag catggtctgc tcgtgattgc
     3841 ccatcagggc gcgaacctcg aacacgcccc gttcggctgc ggcggtggtc agggcgatca
     3901 gacggtcgat caccccgcgt gaagccgggc cgcggtccac atagtcaccg acaaacacca
     3961 gcacgggctg atcctgacgg ttcagcgcgg cgaaatcccg cgtgatgacg cccagcagat
     4021 cctccaggag gtccagatgg ccgtggatgt cgccgaccgc ataaaccacg cgtccgcccg
     4081 tggacgcgct ggccgtcggg ctgggcttgg ggcggaaaaa cttgaacttc atcatctgtc
     4141 cacttgggcg cttcaaccgc ccactgaggc ggagcgcaac ttgcctgaga caacaagcgc
     4201 catcaaccac ccgccaaaaa tcgttaccag ccagtatacc acattctaat acagagcgtt
     4261 caccacagtt attggcttgt cgttattcct aatattctag ttaccatatg tattcattcc
     4321 caaatcacca gtctttttaa gctgataatt gcggcttaat tgcattgttg cgtcgtcatt
     4381 aagggcgatg tttcgatgaa tatccgcaaa caactcatcc ggttcgcaag tgttgctgtc
     4441 atgctggcgg tcaccacgac ggcgcatgct ggagcccgcc cgttcatggc gcgcggcgaa
     4501 acggtcgagg cgccccctgg cttcaccgac ctgtgccagc gggatgaaac cacctgcgga
     4561 tctccggccg ccatggtcgc gccgaccagc gccctggcca tgaccgcgct gagcaccgcg
     4621 tcgctgggcc ggacctggcc gatggccatg gccgccgccg agcgccaggc ctcggacgcg
     4681 gccgccgagg ctaccgccct gggcctgacc gtcgtcgaaa gcctgcaagc ctaccgcccc
     4741 gaggcggtcg aggacttcct gcccgccggc gctgttcgcc aggccgcgcc cgccccgacc
     4801 gtcatggctg cgaccgccag ccctggtctg ctgaacaaga gccagttgaa gctgatcacc
     4861 accatcaatc gcgacgttaa ccgcgacatc cgcaaggcca acgacttcga catctacggc
     4921 atgccggagt tctggtcgct gccccgggtg atcgacggca agatgtacgg cgactgcgaa
     4981 gactatgcgc tggagaagcg ccgccgcctg atcgaagccg gcgttcctgt cgaggccctg
     5041 tccatggccg tggcggtgac cctgcgcggc gagcgccatg ccgtcctggt cgtggccttc
     5101 gagggcggcg acatggtcct ggacaacctg acgccctggc ccaccccgtg gggtgagctg
     5161 aactacacct ggatccagcg ccagatcgcc ggctccgccg cctggacgac aatcagctag
     5221 acccgttcct cgcgtccgtc gccaaccaga aggatggcgg cggacagaga gcccgacagc
     5281 aaaaagcccc ggagcacagg ctccggggct ttttgcttct tccgtcggtc gaacggtagc
     5341 tagacgataa ggcggatcgc ctcgacggcc aggctccaga gacccgccga gacgacagca
     5401 gcggacagcc agcccacggc gcgcggcaag cgccgaacag cggcgaagcc gctcgcggca
     5461 gcggatggcg tgacagaaag gtcggccggg atcatcgaca tggggtgctc ctgtggcctg
     5521 cagcctagga gagggtgacg tgacccccat ttctcatcca gcgataacga gagtcctggg
     5581 ttgatttgac cttgccccca accttggacc aggtcgggcc gggatttctc gccggtcagg
     5641 cgccgggcgg gccatatggc ccggcgccgt tcatcagctc gatcgggcac ttgtcgccga
     5701 tcgcgctgtg gggacgatgt tcattatagt ccctacgcca agcctcgcat tttgagcggg
     5761 cgtcgtcgag ggacatgaac caggcggtgt tcaggcattc ggcccggacc ttgccgttga
     5821 aggactcggc aaaggcgttg tccgtcggct tgccggggcg gctgaagtcc agggtgacgc
     5881 cgcgctgata ggcccacaga tccaggtcgc gggagacaaa ttccgggccg ttgtcgaccc
     5941 gtatggtctt gggataaccc accaccttgc cggcccgatc gagggtctcg acaacgtcgt
     6001 gagccttgta agcgaagcgg ggatccaggg ccggcgagaa ccggctgaag gtgtcgatga
     6061 ccgccagaat gcggatcttg cgcccgtcga acagctgatc gtgcaggaag tccatggccc
     6121 agacgtcgtt gggccgggag gccggttgac gatcctcgcg cagcttggcc ttgaccttgc
     6181 gcttgggcgt cttgttgcgc agttgcaggc ccaactcctt gtagaggcga tatattttct
     6241 tgccattgac cggccatccc tcgcgtttga gcagcaccgt gatccgccgg tagccgtagc
     6301 gcacccgcgt ctcggcgatc tcttttatcc gcttcttcag ggcgacagga tcggtccgct
     6361 tgcccttgta gtgatagctg gatcgttccg cctggagcgc cccgcaggcg cgccggatgc
     6421 tgacctgcca ggccgctcgc agatagtcca cgagctggcg gcgccgggca ggtcctagag
     6481 ctttcgcttg atgacgtcct gcagcatctc cttgtcgagc gacaggtcgg ccacgatccg
     6541 cttgagtcgg gcgttctcgt cctcaagctg gcgcagtcgc ttcatctccg atggcatcag
     6601 gccggcgtac ttcttgcgcc agacgtagaa cgtcgcctcg gaaatccccg ccttgcggca
     6661 gacctcgcca accgtggcgc cctcatcgac ctgacgcagg atgaacgcca cctgcgcctc
     6721 actgaacttc gacctcttca tggtcgctcc tcgctccgtg gcccttgccg caaaacgaga
     6781 aaattctagc tcaaaccggt ccagtttttc aggagcaggt caagcgatca cagcaagcgc
     6841 catgaacttg aaccctaccg agaccacgta gccgatagcc ttctctgcca agaaggatgt
     6901 tttagagagg acaccaaacg gcactacgac aaaggcgatc agggtgacga tgtagaattc
     6961 gatgatcgta acggcgatct cgatcgccag aatgatgaac gccaacagga tgccgatcgc
     7021 ggcgacagcc gcgacgatga tcatgtcgat atgcatgagc gcgccgaagc cagcgttttg
     7081 ctgcgctagg tcaccgatgt atctgatgag gtcgattacg accgagaggc ccgcttcgac
     7141 cgccttagtc ggcgcccgcg tgaaatcgcc gatcgagccg ccaccgctgg cctgcaagcc
     7201 tagctttgca aatccattga tgaccgtaag ggtcagcgag tgccagttcg tgaccagcca
     7261 agcgaagaac ccgatcagga gtatcttcct gacgagcgca gccatgatgt tttgcgtctc
     7321 gtcgatcgcc caaaggatcg ctgagatcgt gatgctgatg accaccaaag tcgccagtac
     7381 gccctggacg ggccccgcaa tggctccgaa gccactgccc acctggcccg tgaacgcact
     7441 gatgaattga tcgagagcag caggatctgc ggtggccatg gacgaactcg gcgcactacc
     7501 agcctagagg ctgtttttgt cgggaaggct gctgcccttc ttcttgaagt acagcggtga
     7561 acatggatcg ttccggtcac gattgttgat gtccggacac ggcttcgcct tggctggcgg
     7621 gtcggtttga ttcggctctg tggcgggctt tccgcagccc gcgaggccaa cggcaacgag
     7681 acagctgatg acaagcaacg accgcatcat tggaaatccg agatgttcgg cagccggctc
     7741 ccagtctttg tgtaagccgt agcccgctcc gtctcagctc gggccgcgat cgccccggcc
     7801 tgctgctgag ccaggatcgt ctcctgggtc ctcgcttgcg cgatcaggat cgtctgaagg
     7861 ccctgaagct ggctgctgag cgcggccaac aactggttgg tcgcctggat cgccgccgtt
     7921 tgtccggcgg cgccttgcga ggcggccacc gcgccgttga cggcgccggc cgtggtgccc
     7981 tgcgacctga ccagctggtt ctggacagcc atcgagtcct gcaaggtcag tcggctgttc
     8041 gcctcccagc tcgctagccg ctgctggatt tgagcgaagc tctgcccgga caggctcgcg
     8101 gggtagaact gctggaactg gccggcgata ttttggctgt tgtagccgat cgcctgggcc
     8161 tgctgcatga gggcgttgag gtctttcatg cgccctagat aggcggttcc accgcgcggc
     8221 gccaagggcg ctgacggcga ccgcccgtca cgtcacccca ggcgt
//
LOCUS       DAOMEO010000011         8153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1363_length_8153_cov_2.401334, whole genome shotgun sequence.
ACCESSION   DAOMEO010000011 DAOMEO010000000
VERSION     DAOMEO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_8153_cov_2.401334"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1176)
                     /locus_tag="PLH31_00515"
     CDS             complement(136..1176)
                     /locus_tag="PLH31_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR87770.1"
                     /translation="MNKLLGATAALALLAAADTAYAARDYVWAAGSSTVFPFTTRVAE
                     NYAKKTGNKSPKVESLGTGGGFKMFCGGTGEKYPDIANASRPMKKSEFEACQKAGVKD
                     IIELKIGFDGIVVAMDKKAPDYNFKLEHLYLGLADQTLRGGQLVENPYKSWNEIGPGL
                     PNTRILAYGPPPTSGTRDAWIELGIEGGAAKLSTLAKLKASDDKTFKAKVAPMRTDGG
                     WVDAGENDNAIIGTIQKTPNAIGVFGYSFLEQNANNIKAATIEGVEPSVQTITSGAYP
                     ISRSLFIYVKKSQIGVTPGLREFVSEFVSDAATGRGGYLQSRGLIPLPPVQHEANKAK
                     SKALPAMPAPES"
     gene            complement(1289..>1468)
                     /locus_tag="PLH31_00520"
     CDS             complement(1289..>1468)
                     /locus_tag="PLH31_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQR87771.1"
                     /translation="ATDEAILDAMVEHPILVNRPIVVTPKGVALCRPSEAVFPLLEKA
                     PDSFTKEDGEVVSRV"
     assembly_gap    1469..1478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1479..1706)
                     /locus_tag="PLH31_00525"
     CDS             complement(<1479..1706)
                     /locus_tag="PLH31_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HQR87772.1"
                     /translation="MSNADFPVVIFHNPACGTSRNVVAMVEAAGYAPQVVEYLKAGWT
                     REQLRELAGEAGLSLRALMREKGTPAAELGLL"
     gene            complement(1703..2788)
                     /gene="arsB"
                     /locus_tag="PLH31_00530"
     CDS             complement(1703..2788)
                     /gene="arsB"
                     /locus_tag="PLH31_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQR87773.1"
                     /translation="MSVFERYLTLWVALCIVAGVALGHFFAPAFHLIASAEIANVNLP
                     MAGLIWLMIVPMLVKVDFAALGEVRNHWRGIGVTLFINWVVKPFGMAALGWIFIANLF
                     RPWLPADQIESYIAGLILLAAAPCTAMVFVWSNLTKGEPNFTLSQVALNDTIMVVAFA
                     PIVGLLLGLSAITVPWSTLTLSVGLYIVVPVLAAQLIRKALLNAGGQPALDALMARLQ
                     PLSMIALLATLVLLFGFQGEQILRQPLVIAILAVPILIQVYFNSGLAYLLNRMTGEAH
                     CVAGPSALIGASNFFELAVAAAISIFGFQSGAALATVVGVLIEVPVMLSVVAIVNASK
                     AWYERGPAVRKVAAAREPSPQPQTGDV"
     gene            complement(2798..3169)
                     /locus_tag="PLH31_00535"
     CDS             complement(2798..3169)
                     /locus_tag="PLH31_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR87774.1"
                     /translation="MEMTSAVASLSALAHGGRLSAFRMLVQAGAAGLPAGEIARCLKM
                     LPNSLSSHLAILDKAGLIHARREGRSIIYTADYGAMQELLGFLLEDCCGGAPEICAPL
                     GEIFARAVACHGTCLPELETA"
     gene            3224..3943
                     /gene="arsH"
                     /locus_tag="PLH31_00540"
     CDS             3224..3943
                     /gene="arsH"
                     /locus_tag="PLH31_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HQR87775.1"
                     /translation="MTDFPALDARYLDAPATARLLAGAPDHPPRILLLYGSLRERSFS
                     RLLVEEAARILQRLGAETKIFDPRDLPAPDSVDASHPKVQELRALSLWSEGQVWCSPE
                     RHGAITGVIKSQIDWIPLEMGSVRPTQGRTLAVMQVSGGSQSFNAVNTLRLLGRWMRM
                     ITIPNQSSVAMAWKEFDDNDRMKPSSYYDRVVDVMEELVKFTLLTRGHADYLVDRYSE
                     RKASGDAAPLNAPAAAVARGA"
     gene            4065..4820
                     /locus_tag="PLH31_00545"
     CDS             4065..4820
                     /locus_tag="PLH31_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQR87776.1"
                     /translation="MGHPTKAIILSAGQGKRLSPLTDTRPKCLVELSGRTVLHWQLRH
                     LRQAGITEAVVVTGFAAETVEAEIGGLDLPGMTVRTLFNPFFGLTDNLATCWLARGEM
                     VGDFLLLNGDTLFEPAIAERLIAAPPARITVTIDRKGGYDADDMKVLTDGLSLRAIGK
                     TIETYDAESIGFLRFDPEGAALFTAIVEAALRTPEGLKRWYLSVINQIAQEHDVVRVQ
                     SIQGLDWAEMDFPEDLPRNRELAASWVAELVGA"
     gene            complement(4882..5631)
                     /locus_tag="PLH31_00550"
     CDS             complement(4882..5631)
                     /locus_tag="PLH31_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR87777.1"
                     /translation="MSFQALVLAGSRGGVDPVADYAGVSHKGLIVLGGQTLLTRVLRA
                     LDQAGATRIGVSTSDIAVVSALEGQDRVSPFPAAGGPSQSVHDGASLMGTPLLVTTVD
                     HALLQPEWMTEFLTKAPADCDIAILLAPEAVVRAAAPETKRTYLKFKDGRFSGCNLFL
                     LRNETALNAVVFWQTVEGLRKQPWRIAAMIGPMMLLRYALGLMTLDETLERLGRAAGV
                     RAAAVRSSYGLAAVDVDKPADLDLVRMIVER"
     gene            complement(5628..6779)
                     /locus_tag="PLH31_00555"
     CDS             complement(5628..6779)
                     /locus_tag="PLH31_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP pyrophosphohydrolase"
                     /protein_id="HQR87778.1"
                     /translation="MPFDSHGADITIVPVRTRAELTRFIKLPIRLNAGDPNWITPLMM
                     ERAEALSPKTNPFFEHAEVQLWLAVRGGRDVGRISAQIDALAPQTPGLKTGNFGLIAA
                     EDDAVIFSLLFRAAEDWLRARGCVRALGPFNLSVNEEVGLLVDGFDTPPMVMMGHDPA
                     YTGGRVEEQGYAKAKDVYAYLCDPTADLPAVVVRRVRRGLPDGVTLRELDMSRYDEEV
                     VSLTEILNDAWSGNWGFVPTTEAETRQLAKALKPVIEKKMTLFAEIDGEPAGFIVLLP
                     NVNEAIADLKGKLLPFGWAKLLWRLKVSGVKSARVPLMGVKRKFADTMRGQLLPFHLM
                     NAGRNASLALGYTQFEFSWVLEENMPMRRISEAMGATIYKTYRIYEKTL"
     gene            complement(6779..7579)
                     /locus_tag="PLH31_00560"
     CDS             complement(6779..7579)
                     /locus_tag="PLH31_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQR87779.1"
                     /translation="MRAAGISAYRIVAMAAPAALAVMLLDFACAQVIAPRTDPILDAW
                     WKAHTPAADRKAPGPVSFRVGDDLVIAASSSPDGRTLEAVKIYRRDNTGRLIERIEAP
                     SATADTRGWRLSKAVTTRFAGERADTVTTSQMTWASSLRPEDIQALFSENPAPSAGSA
                     RRALLNGGTDRPPSYYETRLWSAYAGPFGALIMLLLSAPVALANFRSSQGATLLAAGL
                     AAGLVFLVIDGLLTALGESGAVSPILAVWGGPVIFGALAINALINLEG"
     gene            complement(7837..8153)
                     /locus_tag="PLH31_00565"
                     /pseudo
     CDS             complement(7837..8153)
                     /locus_tag="PLH31_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
BASE COUNT         1403 a         2653 c         2745 g         1342 t
ORIGIN      
        1 aaagctctcc gaaatcggcg ctcgtgtcat ggggcgctca aggctgcgcg cggtcaaggt
       61 ctttgggtgt ggttgcacga ttgtgaagcc ggcaaacggc gtcatcccgg atcaggtccg
      121 ggatgacgcg caagatcagg actccggtgc gggcatggcg gggagcgcct tggacttggc
      181 cttgttggcc tcgtgctgaa cgggcggcag agggatcagg ccgcgcgatt gcagatagcc
      241 cccgcggccg gtagcggcgt cggacacgaa ctcgctgacg aactcgcgca ggcccggcgt
      301 cacgccgatc tggctcttct tcacgtagat gaagagcgaa cgcgagatcg ggtaggcccc
      361 gctggtgatc gtttggacag atggctcaac gccttcaatg gtggcggcct tgatgttgtt
      421 ggcgttctgc tcaaggaacg aatagccgaa cacgccgatg gcgttgggag tcttctggat
      481 agtgccgatg atggcgttgt cattctcgcc ggcgtcgacc cagccgccat cggtgcgcat
      541 cggcgcgacc ttcgccttga aggtcttgtc gtcgctggct ttcagcttgg ccagggtgga
      601 aagctttgcg gcgccgccct cgatgcccag ttcaatccag gcgtcgcgcg tgcccgaggt
      661 gggcggcggg ccataggcca ggatgcgggt gttgggcagg ccgggaccga tttcgttcca
      721 gctcttgtag gggttctcga ccaactggcc gccgcgcaag gtctggtcgg ccaggccaag
      781 gtaaaggtgc tcaagcttga aattgtagtc aggagccttc ttgtccatgg ccacgacgat
      841 gccatcgaag ccgatcttca gctcgatgat gtccttgacg ccggccttct ggcaggcctc
      901 gaactcggac ttcttcatcg ggcgtgaggc attggcgata tcggggtact tctcgcccgt
      961 gccgccgcag aacatcttga atccgccgcc cgttcccagg ctttcgacct tcggcgactt
     1021 gttgccggtc ttcttggcgt agttctcggc cacgcgcgtc gtgaacggga acacggtgga
     1081 ggagccggcg gcccacacat agtcgcgcgc ggcataggcg gtgtcggcgg cggccagcag
     1141 cgcgagggcc gcagtggctc ccagaagctt gttcatgact ttgtttccgt gtcgtcgaag
     1201 agcgcagtaa actcttaggt cagcgggctt ttgtcacgct ttaatgacgg ctttgcgaca
     1261 tccgcacggc gcgaggaggt gtcaccgttc agacccgtga tacgacttcg ccgtcttcct
     1321 tggtgaagct atcgggagcc ttttccaata gcggaaacac cgcttccgac ggccggcaca
     1381 gggccacgcc cttgggtgtc accacgatgg gccggttcac caggatcgga tgctcgacca
     1441 tggcgtccag gatcgcctcg tcggtcgcnn nnnnnnnnca gaaggcccag ctcggcggcg
     1501 ggcgtaccct tctcgcgcat cagcgcgcgc aggctcaggc cggcttctcc ggccagttcg
     1561 cggagctgct cgcgggtcca ccccgctttc agatattcga ccacctgggg cgcgtagccg
     1621 gcggcctcga ccatcgctac cacgttgcgc gaggttccgc aggcggggtt gtggaagatc
     1681 acgacgggga aatcagcgtt gctcatacgt caccggtctg gggttgaggg gagggttcac
     1741 gggccgccgc gaccttgcgc acggcggggc cgcgctcgta ccacgccttg ctggcgttca
     1801 cgatggcgac gaccgacagc atcacgggca cctcgatcag cactccgacc acggtcgcca
     1861 gggcggcgcc ggactggaaa ccgaagatgc tgatggcggc ggcgacggcc agttcaaaga
     1921 agttgctggc cccgatcagg gccgagggcc cggccacgca gtgggcctcg ccggtcatgc
     1981 ggttgagcag ataggccagg cccgagttga aatagacctg gatcaggatc ggcacggcca
     2041 ggatggcgat gaccaggggc tggcgcagga tctgctcgcc ctggaagcca aacagcagca
     2101 ccagcgtcgc cagcagggcg atcatcgaca gcggttgcag tctggccatc agggcgtcca
     2161 gagctggctg gccgcctgcg ttcagcaggg ccttgcggat caactgggcc gccagcacgg
     2221 gaaccacgat atagagcccc accgacaggg tcagggtcga ccacggcacg gtgatcgccg
     2281 acagacccaa cagcaggccg acaatcgggg cgaaggccac gaccatgatg gtgtcgttca
     2341 gcgccacctg gctgagtgtg aaattgggtt cgccctttgt caggttgctc cagacgaaca
     2401 ccatggccgt gcagggcgcg gccgccagca ggatcaggcc ggcgatatag ctctcgatct
     2461 ggtcggcggg cagccagggg cgaaaaaggt tggcgatgaa gatccagccc agggccgcca
     2521 tgccgaacgg cttgaccacc cagttgatga acagggtcac gccgatgccg cgccagtggt
     2581 tgcgcacctc gcccagtgcg gcgaaatcca ccttgacgag catgggaacg atcatcagcc
     2641 atatcagccc cgccatgggc aggttgacat tggcgatctc ggccgaggcg atcagatgga
     2701 aggcgggcgc gaaaaaatgg cccagggcca cgccggcgac gatgcacagc gccacccaca
     2761 gggttaggta gcgttcaaag acggacatgg cggtcggtca cgcggtttcc agttcgggca
     2821 ggcaggtccc atggcaggcg acggctctgg cgaagatctc gccgaggggc gcgcagatct
     2881 ctggcgcgcc gccgcagcag tcttccagca ggaagcccag cagctcctgc atcgcgccat
     2941 agtcggcggt atagatgatc gagcggccct cgcgccgggc gtggatcaga ccggctttgt
     3001 cgagaatggc cagatggctc gacaggctgt tgggcagcat tttcaggcac cgtgcgatct
     3061 caccggccgg cagtccggct gcgccggcct ggaccagcat ccggaacgcc gaaaggcggc
     3121 cgccatgggc gagggctgaa aggcttgcga cggcgctagt catttccata tttcgacgat
     3181 aatcgaaatg tatgacattg tgaagatgtc tcccggagcg cccatgaccg atttccccgc
     3241 cctcgacgcc cgctatctgg atgcgcctgc caccgccagg cttctggcgg gggctcccga
     3301 tcacccgccg cgcatcctgc tgctgtacgg ctcgctgcgg gagcgctcgt tcagccggct
     3361 gctggtcgag gaggcggcgc gcatccttca gcgcctgggc gcggagacaa agatcttcga
     3421 cccgcgcgac ctgccggcgc ccgactcggt cgacgccagc catcccaagg tgcaggagct
     3481 tcgcgctctg tcgctgtggt cggaagggca ggtgtggtgc agtcctgaac ggcatggcgc
     3541 catcaccggc gtgatcaaga gccagatcga ctggatcccg ctggagatgg gcagcgtgcg
     3601 gcccacgcaa ggccggaccc tggcggtgat gcaggtcagc ggcggctcgc agtcgttcaa
     3661 cgccgtcaac accctgcgcc tgctgggccg ctggatgcgg atgatcacca tccccaacca
     3721 gtcgtcagtg gccatggcct ggaaggagtt cgacgacaac gaccggatga agccctcgtc
     3781 ctattacgac cgcgtagtcg acgtcatgga ggaactggtc aagttcaccc tgctgacgcg
     3841 gggccacgcc gactatctgg tcgaccgcta cagtgagcga aaggccagcg gcgatgccgc
     3901 gcccctgaac gcgcccgccg ccgctgtcgc gcgaggcgcg tgactgcgac cttccgcctc
     3961 ccaccgggcc gccgcggggc ccctatatgg aggccgctgt atcctgtagc ttcccgccct
     4021 gaatcagacg ccgacgcgtg cggccagaag tgagacgagt ccctgtgggc cacccgacca
     4081 aagccatcat cctgagcgcg ggccagggca aacgtctgtc gcccctgacc gacacgcgcc
     4141 ccaagtgcct ggtcgagctg tcgggccgca ccgtgctgca ctggcagctg cgccatctgc
     4201 gtcaggccgg gatcaccgag gccgtggtcg tcaccggctt cgctgcggag acggtcgagg
     4261 ccgagatcgg gggtctcgac ttgcctggca tgacggtgcg caccctgttc aacccgttct
     4321 tcggcctgac cgacaacctg gctacctgct ggctggcgcg cggcgagatg gtcggcgact
     4381 tcctgctgct gaacggcgac accctgttcg agccggccat cgccgaacgc ctgatcgccg
     4441 ccccgccggc acggattact gtcaccatcg accgcaaggg tggctacgac gccgacgaca
     4501 tgaaggtgtt gaccgatggc ctcagcttgc gcgccatcgg caagaccatc gagacctatg
     4561 acgccgagtc gatcggcttt ctgcgcttcg atcccgaggg cgcggcgttg ttcacggcga
     4621 tcgtcgaggc ggccctgcgc acgcccgagg gcctgaagcg ctggtatctc agcgtcatca
     4681 accagatcgc ccaggaacac gacgtggtcc gcgtgcagtc gatccagggc ctcgactggg
     4741 ccgagatgga tttccccgaa gacctgccgc gcaaccgcga gctggcggcg tcttgggtgg
     4801 cggagctggt cggggcgtaa ggctcctctt ataaatccgc tcttcccggc cttcgccggg
     4861 gaaagcgggc aatggattgg ctcagcgttc gacgatcatc cgtaccagat ccagatccgc
     4921 cggcttgtcg acatccaccg ccgccagccc gtacgaagac cgcaccgccg ccgccctaac
     4981 cccggccgct cggcccagcc gttccagggt ctcgtccagg gtcatcagcc ccagggcgta
     5041 gcgcagcagc atcatcgggc cgatcatcgc cgcgatgcgc cagggctgtt tgcgcagtcc
     5101 ctcgaccgtt tgccagaaga ccacggcgtt cagggctgtc tcgttgcgca gcaggaacag
     5161 gttgcagccc gaaaaccggc cgtccttgaa cttcagatag gtccgcttgg tctctggcgc
     5221 ggccgcgcgg accaccgcct cgggggccag caggatggcg atgtcgcagt cggccggggc
     5281 cttggtcagg aattcagtca tccattcggg ctgcagcagg gcatggtcca ccgtggtgac
     5341 cagtagcggc gtccccatca ggctggcgcc atcgtgcacg ctctggctgg gaccgccggc
     5401 agcagggaag gggctgaccc tgtcctgccc ttcgagcgcc gaaacgaccg cgatgtctga
     5461 ggtcgagacc ccgatccgcg ttgcgccggc ctgatccagc gcgcgcagca cccgggtcag
     5521 cagggtctgg ccgcccagga cgatcaggcc cttgtgactg acgccggcat agtcggcgac
     5581 cgggtcgacg ccaccacgcg agcccgccag aaccagtgcc tggaagctca cagcgttttc
     5641 tcgtaaatcc gataggtctt gtagatcgtc gcgcccatgg cctcggagat ccgccgcatc
     5701 ggcatgttct cctccagcac ccacgagaac tcgaactggg tgtagcccag cgccagcgaa
     5761 gcgttacggc cggcgttcat caggtggaac ggcaacagct ggccgcgcat ggtgtcggcg
     5821 aacttgcgtt tcacgcccat cagcggcacc cgcgccgact tcacgccgct caccttcagc
     5881 cgccacagca gcttggccca gccgaacggc agcagcttac ccttcaggtc ggcgatcgcc
     5941 tcgttgacgt tgggcagcag cacgatgaag cccgccggct cgccgtcgat ctcggcgaac
     6001 agggtcatct tcttctcgat gacgggtttc agggccttgg ccagttgccg cgtctcggcc
     6061 tcggtagtcg gcacaaagcc ccagttaccc gaccaggcgt cgttgaggat ttcggtcagc
     6121 gagacgacct cttcgtcgta gcggctcatg tccagctctc gcagcgtcac gccgtcgggc
     6181 aggccgcgcc tgacccggcg caccaccacg gccggcaggt cggcggtcgg gtcgcacaga
     6241 taggcataga cgtccttggc cttggcgtag ccctgctcct cgacacggcc gccggtatag
     6301 gccgggtcgt ggcccatcat caccatgggc ggggtgtcga agccgtcgac cagcaggccg
     6361 acctcttcat tgaccgacag gttgaagggc cccagcgctc ggacgcagcc gcgcgcccgc
     6421 agccagtcct cggcggcgcg gaacagcagg ctgaagatga ccgcatcgtc ttcggccgcg
     6481 atcaggccga aattgccggt cttcaggccg ggcgtctgcg gcgccagggc gtcgatctgg
     6541 gcgctgatcc ggcccacatc gcggcccccg cgcacagcca gccacagctg cacctcggcg
     6601 tgctcgaaga acgggttggt cttgggcgac agggcctcgg cccgctccat catcagcggc
     6661 gtgatccagt tgggatcacc ggcgttcagg cggatgggca gcttgatgaa gcgcgtcagc
     6721 tcggcgcggg tgcgaaccgg cacgatggtg atgtcagcgc catgagagtc gaacggcatc
     6781 agccctccag attgatcagg gcgttgatgg ccagggcccc gaagatcacc ggaccgcccc
     6841 agaccgccag gatgggcgag accgcgccgc tttcgcccag cgccgtcagc agaccgtcga
     6901 tcaccagaaa caccaggcca gccgccagtc ctgctgcgag tagcgtcgcg ccctggctgc
     6961 tgcggaaatt ggccagggcg accggcgcgc tcaacagcag catgatcaac gccccaaagg
     7021 gtccggcata ggcgctccag agacgggtct catagtagct gggcggtcgg tcggtgccgc
     7081 cattcagcag cgcgcgacgg gctgaaccgg ctgaaggggc ggggttttcg gaaaacagag
     7141 cttggatatc ctcggggcgc agggacgaag cccaggtcat ctggctggtg gtcacggtat
     7201 cggctcgctc gccagcgaag cgggtggtca cagccttcga caggcgccag cccctggtgt
     7261 cggcggtggc cgaaggcgcc tcgatccgct cgatcaagcg gccggtgttg tcgcgtcgat
     7321 agatcttcac cgcctcaagg gtgcggccgt ccgggctgct gctggcggcg atcaccagat
     7381 cgtcgccgac cctgaaactg acggggccgg gggccttgcg gtcggcggcg ggagtgtggg
     7441 ccttccacca ggcgtcgagg attggatcgg tccgcggggc gatcacctgt gcgcaggcga
     7501 agtccagcag cattactgcc aacgccgccg gggcggccat ggccacgatg cgataggccg
     7561 agatgccggc cgcccgcatg gcgatcacgg cgctctcgcg ggccagctgc gaaaaggcga
     7621 acaggccgcc ggccagcacg gcgatcgggg cgacctgctc gaacaggcga ggcatgcgca
     7681 ggacggcgta atagaccact ccgcccaggc ccaggccccg gtcgaggatg tcggtggtca
     7741 cctccagcag gtcgaggatc tgcaggatgc ccatcagaat ggcggcggcg ccgacgatgc
     7801 ggatcgcggc gacgctcagg acgtaggatt gcagcctcat gatgcggcct gtcgtttcgg
     7861 agcgaccatg cgcaccgctc tgtcgatagt ttcgccgatc cggcgcagga ggcggctgac
     7921 aggggaacat ccacacgctg aagctggtga agatcaggaa cggcccccag atcgccagca
     7981 ggggattggc cttgccggcc tcggcgaggc tctggcccat cagcaacgag tgctggaagg
     8041 ccagcagcag gatgccggcc acgaccagac ccggggtgcg gtcgccgcgc tttgcggcca
     8101 gaccgagagg aaaggccagc aggggcagga acgggaggaa cgcggcgcgc gcc
//
LOCUS       DAOMEO010000012         7506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1446_length_7506_cov_2.521675, whole genome shotgun sequence.
ACCESSION   DAOMEO010000012 DAOMEO010000000
VERSION     DAOMEO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_7506_cov_2.521675"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="PLH31_00570"
     CDS             <1..1524
                     /locus_tag="PLH31_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87780.1"
                     /translation="FALINNWDTDLAKNQAIYLQDSWDVLPNLTLNLGVRRDQFQNRG
                     INLGGVKGGVFVDFDNEIAFRLGATWDPTNDGRNKIFANYGRYYLPVATNTSYRQASA
                     ILDQSAFFLQPAGFQLYLPGTTTVNTAQVNPISGLPIAGLGTQITGFANSAICLPGGV
                     GAAGVRGCTVRNNGTVQPFAANISQNLKSTADDEYILGYEHRFDSLWKAGATLTFRKS
                     LRAADDVAVDTAVVAYCEANGIANCADVYSGFHQYTIINPGLAQTITLADPLPGETTV
                     RTIDFTAEQLKYPKVDRQYIALQLQFDRAFDGKWALNGSYTLSESKGNYEGYVKTDYA
                     GGQTDAGITADFDTPGLTEGAYGLLPNHRAHTFKAFGSYQVTDQILVGANALVTSGRK
                     YGCMGFNPNDPILNSAYGALSHYCNGVSSPRGSVFSDPWTYRLDLSARYTVPSFGFLP
                     ENGLTLRADVFNVFNTRKTVESNEFGELDSGATNVNFQAPLAYMTPRSVRLGFDLVF"
     gene            complement(1657..3123)
                     /locus_tag="PLH31_00575"
     CDS             complement(1657..3123)
                     /locus_tag="PLH31_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR87781.1"
                     /translation="MTPSASDLLAEAVRAFQARDRDYAAGLLARLNAEDAALGDDWGA
                     VARLAATLGEVSVALAAIRRYVALKPYDVERRLEYGALLAQCGRIEGAAKATRAFLPN
                     APADPRLYLLLGTCQAQLGQTEAALADLRAVLALDPQPALAGAAWLAIADLKRFTEDD
                     PDIAALKAVIASTDPATPSSLNFALGKALDDIGDVDAAFAAYEAGARAARALNPPNLA
                     ASRDFVDQVIAGFTRESLAKLPPSAADSDRPIFVLGLPRSGTTLVEQILASHSGVKDG
                     AELNLFRAATMALGGYAPSDVSRLAFFPGTENVWTLFGRVYLHLLDERFGPEGRIVDK
                     TLNHTRMVGLIHHVLPKARFVWLRRDPADSALSCFRSHFVGGLNWAWSMRDIGAHFAD
                     EDRLHAHWTQQFPDAILTVPYEDLVKDPETWIARILEHCGLPFQAGVRDFHLTERAVT
                     TSSSSQVRQPLNVRGIGAWRRYSQHMQPFFEAYGSRPS"
     gene            complement(3206..4931)
                     /gene="sppA"
                     /locus_tag="PLH31_00580"
                     /pseudo
     CDS             complement(3206..4931)
                     /gene="sppA"
                     /locus_tag="PLH31_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
     gene            complement(5051..5127)
                     /locus_tag="PLH31_00585"
     tRNA            complement(5051..5127)
                     /locus_tag="PLH31_00585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5091..5093),aa:Pro,seq:cgg)
     gene            5279..5352
                     /locus_tag="PLH31_00590"
     tRNA            5279..5352
                     /locus_tag="PLH31_00590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5311..5313,aa:Gln,seq:ttg)
     gene            5705..6145
                     /locus_tag="PLH31_00595"
     CDS             5705..6145
                     /locus_tag="PLH31_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HQR87782.1"
                     /translation="MEDKASLIELTAEIVANYVANNSTPVSDLPALIRATHDALAGIG
                     SPEAPVVEAVTKPTAAQIRKSITPDALISFEDGKPYKTLKRHLTTHGMTVAEYKAKWG
                     LPNDYPTTAPAYSEARSAMAKALGLGQGGRKAKSPAKAPRARKA"
     gene            complement(6206..7276)
                     /locus_tag="PLH31_00600"
     CDS             complement(6206..7276)
                     /locus_tag="PLH31_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR87783.1"
                     /translation="MAQIMEAGALDAEQKQRLSVITRSGHSLLGVINAILDISRIEAG
                     RMEIIPTPFSLEGVLETLQQLYGGLAREKGLTFSLDIARSAAGWRLGDPERLRQVLSN
                     LISNAIKFTDIGEVRVSVAGDTTTLIFTVEDTGGGIPESQRDLVFGKFAQLDSSSTRR
                     AGGAGLGLAICKELVGLMGGQIDFSALPGGGTCFRFSVAMREVEAVASAAAPLIAAPV
                     DEQAPPRILVVDDNDTNRTVLLTLLSHLGVSGQFAVDGRDALAMWEGHRWDAILMDIH
                     MPGMDGVEATRAIRAREGSTGRARTPIIAVTASVLTHERSLYIQAGMDAIVAKPIEVS
                     QLVEILTRTFAAPAPEAGRASA"
     gene            complement(7291..>7506)
                     /locus_tag="PLH31_00605"
     CDS             complement(7291..>7506)
                     /locus_tag="PLH31_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR87784.1"
                     /translation="MGLAGAFAALGMSTLMRGVVGREIRARLASDALNADLQRELQEN
                     RRLQAKAEALALAAQAANRAKTDFLAT"
BASE COUNT         1301 a         2481 c         2456 g         1268 t
ORIGIN      
        1 ttcgcgttga tcaacaactg ggacaccgac ctcgcgaaga accaggccat ctacctgcaa
       61 gactcgtggg acgtcctgcc caacctgacc ctgaacctgg gcgttcgtcg cgaccagttc
      121 cagaaccgcg gcatcaacct cggcggcgtc aagggcggcg tctttgtcga cttcgacaac
      181 gaaatcgcct tccgcctcgg cgcgacctgg gatccgacca acgacggccg caacaagatc
      241 ttcgccaact acggccgcta ctatctgccg gtcgcgacga acacctcgta tcgtcaggcc
      301 tcggccatcc tggaccaaag cgcgttcttc ctgcagccgg ccggcttcca gctgtacctg
      361 ccgggcacga ccacggttaa caccgctcag gtcaacccga tctcgggtct gccgatcgcg
      421 ggtctcggca cgcagatcac cggcttcgcc aactctgcca tctgccttcc gggcggcgtg
      481 ggcgctgcgg gcgtccgggg ttgtacggtt cgcaacaacg gcaccgtcca gccgttcgcc
      541 gccaacatca gccagaacct gaagtcgacg gccgatgacg aatacatcct cggctacgag
      601 caccgcttcg actcgctgtg gaaagccggc gctacgctga ccttccgcaa gtcgctgcgc
      661 gcggccgatg acgtcgccgt cgacaccgcc gtcgtcgcgt actgcgaagc caatggcatc
      721 gccaattgcg cggacgtgta cagcggcttc caccagtaca cgatcatcaa cccgggtctg
      781 gctcaaacca tcaccctggc cgatccgctg ccgggcgaaa ccaccgttcg caccatcgac
      841 ttcaccgctg aacagctgaa gtacccgaag gtcgatcgtc agtacatcgc gctgcagctg
      901 cagttcgacc gtgctttcga tggcaagtgg gcgctgaacg gttcgtacac cctctcggaa
      961 tcgaagggta actacgaagg ctacgtgaag accgactacg ccggtggtca aaccgacgcc
     1021 ggcatcaccg ccgacttcga cacccccggc ctgaccgaag gcgcctacgg cctgctcccc
     1081 aaccaccggg cgcacacctt caaggccttc ggttcgtatc aggtcaccga ccagatcctg
     1141 gtcggcgcca acgccctggt cacctcgggt cgcaagtatg gttgcatggg cttcaacccc
     1201 aacgacccga tcctgaactc ggcctacggc gcgctgtcgc actactgcaa cggcgtctcc
     1261 tcgccgcgcg gttcggtgtt cagcgacccc tggacctatc gcctcgacct gtcggcccgc
     1321 tacaccgtgc cgtcgttcgg cttcctgccg gaaaacggtc tgaccctgcg cgccgacgtc
     1381 ttcaacgtgt tcaacacgcg caagaccgtc gaaagcaacg agttcggcga actggatagc
     1441 ggcgcgacca acgtcaactt ccaggctccg ctggcctata tgaccccgcg ttcggtgcgt
     1501 ctgggcttcg atctggtctt ctagtcagac ctgatcatcg accaagtcag agggcaggcc
     1561 gcaaggcctg ccctttttct ttgggcccag acgaccgggc aaagaaaaag ggcggagacc
     1621 gcggtctccg ccctttttcg tggggtgctg gcagggtcag ctcggccttg agccataggc
     1681 ctcgaagaac ggctgcatat gctggctgta gcgccgccag gcgccgatgc cgcgcacgtt
     1741 caacggctgg cgcacctggc tggagctcga ggttgtcacg gcgcgctccg tcaggtggaa
     1801 gtcgcgcacg ccggcctgga acggcaggcc gcaatgctca aggatcctgg cgatccaggt
     1861 ctcggggtct ttgaccaggt cctcataggg cacggtgagg atggcgtcgg ggaactgctg
     1921 ggtccaatgg gcgtgcagtc tgtcctcgtc cgcgaagtgg gcgccgatat cgcgcatcga
     1981 ccaggcccag ttcagcccgc cgacaaaatg gctcctgaag cacgagaggg ccgagtcggc
     2041 cggatcgcgc cgcagccaga cgaacctggc tttgggcagg acgtgatgga tcaggcccac
     2101 catgcgggtg tggttcaggg tcttgtcgac gatgcggccc tcggggccga agcgttcatc
     2161 cagcaggtgc agatagaccc gcccaaacag cgtccagaca ttctccgtgc cggggaagaa
     2221 ggccagccgc gatacgtcgg acggcgcata gccgcccagg gccatggtcg ccgcccggaa
     2281 caggttcagc tcggccccat ccttgacgcc gctatggctg gccagaatct gctcgaccag
     2341 ggtggtgccc gaacgcggca ggcccagcac gaagatcgga cggtcgctgt ccgccgcgct
     2401 gggcggcagc ttggccaggc tttcgcgggt gaagccggcg atcacctgat cgacaaagtc
     2461 acgcgaggcg gcaaggttgg gcgggttcaa ggcccgggct gcacgggccc cggcctcgta
     2521 ggccgcaaaa gccgcatcga catcgccgat gtcgtccagg gccttgccca aggcaaagtt
     2581 cagactgctg ggcgtagccg gatcggtcga cgcgatcacg gccttcagcg cagcgatgtc
     2641 ggggtcgtct tccgtgaagc gcttcaggtc cgcgatcgcc agccacgccg cgccggccag
     2701 cgccggctgc gggtccaggg ccaggacggc gcgcagatcg gccagggcgg cttcggtctg
     2761 gcccagctga gcctggcatg tgcccagcag caggtacagg cgcggatcgg cgggggcgtt
     2821 gggcaggaag gcgcgggtgg ccttggccgc gccctcgatg cggccgcatt gcgccagcag
     2881 ggcgccgtat tccaggcggc gttcgacatc atagggcttc agcgccacat agcgccggat
     2941 ggccgccagg gcgacggaga cctcgcccag ggtggccgcc aggcgggcca cagcgcccca
     3001 gtcgtcgccg agggccgcgt cctcggcgtt cagccgggcc agcaggccgg ccgcatagtc
     3061 gcgatcacgc gcctgaaagg cgcgcacggc ctcggccagg aggtctgagg cgctcggggt
     3121 catggcagat aatccggaaa catggacgtg agtggcgagg gccgcggacg gcgacgccgg
     3181 ggcgccgtcc gtccgcgatc agggtctagc ggatcggcgt gtcggccaga accgacgcgc
     3241 cgccgggcgc ggcgcgcagg cgggccgtgg ccagttcgtc gagaatggcc tgcgagcgcg
     3301 ggtcgcccag gatccaggcc gaggccgcga gggtctgcat cgaggtcgag ctgatgccca
     3361 gggcctgttc cagggcctcg aacggcgact tgccgccatc catgcgcttg aggcgcacgt
     3421 cgcccttcag gttggccagg ctcttggcct tgtcgaccgc gtcatagaag ccgcccagct
     3481 cgtcgacaag gcccagctgc ttggcctgct cgccggtcca gacgcggccc ttggccagtt
     3541 cgcgcacgcg ctcgggcggc agcttgcgcc cttcggcgac gcgggtgatg aagccggcat
     3601 agatgcggtc catccagccg gcgaacttgg cgcgctgctc gctggtgaag ccgtcgcccg
     3661 cgccaaaggc gccggcatag tcgccgccca cattgatctc gcgggcgtcg acaccaaagc
     3721 gggccagggc ctcgcccacg gcgaacttgc cgccatagac gccgatcgaa ccggtcaggg
     3781 tggtgggctc ggcgatgatc gacgaggccg cgctgctgat ccagtagccg cccgatgcgg
     3841 cataggtgcc catgctgacc accaccggct tgccggccgc cttggcggcc ttcacggcag
     3901 ccaggatctg ttccgaagcg gtgtccgaac cgccgggcga ggacacccgg aagacgatcg
     3961 ccttgacgtc cttgtcggcc gcggcgtcgt ggatcgcctt ggccacgtcg tcggaataga
     4021 ccgtgctgtc gccgccgaac ggcgaggcgg tgttgctggt tccagtgatg atcgggccct
     4081 cggcgccgat caccgcgatg gccgggccgg tcttgaccga agggcgcttg gaccgcgcgg
     4141 cgtagtcgtc gaaatcgatc agctttgcgc ccttgccggc gcgctccaga atctgggtct
     4201 gcaggtcgtt gacctggcca acccggtcga tcaggccctt ggcctgggct tcctgggcgc
     4261 tgtaggggcc agcctccagg tcgcggatca gggcggcggg atccttcatg cggtctgccg
     4321 cagccgccgt cagggtcgag cgatagaccg aacccatcca ggacagcgac gactcgcggt
     4381 gggcctgggt gtagtcgcta tagaggtagg gattgaccgc gttcttgtac tcgtggcgct
     4441 gctcgtagtc ggccttgacg ccgtacttgt cgaagaagcg cttgaagaac agcgactcgc
     4501 tggccacgcc cacggcctgg aacgagctgt cgggctgcat ccagaactcg ctggaggcgg
     4561 cccccagcat ataggtcgag cccctggctg tgcgcataga ctggcttctt gccgaccgag
     4621 cggaaatgct tgaaggcccg gcgcagctca tcggctgtcg ccggcgccat gccgccctcg
     4681 ggcaggcgca cgaacagcgc cttgaccttg tcgtccgtct cggcgcgacg cagggtctcg
     4741 atcaccgaca tcacagagcc accgccgccg ccaaacgcag cgaaggggtt ctgcgccgcc
     4801 tgatcggtca ggccgctgcg cagatccaga tgcagaaccg agtgggcggg aaccggggcc
     4861 gggcgcgcgg cgccggcggc catgacgatc aacaggaagg gaacgccgac ggtcaggaag
     4921 aactgcttca tcgaagatcc cgtcaccaat tcagtcgcaa gctaacgact gtcgcgtccc
     4981 ggtacaaatg aacagcgcgc gcacactgtg caagggacag gaagaaaaag ggattttaag
     5041 gcgggaaaaa tggtcggagt ggcaggattt gaacctgcga cccctgcgtc ccgaacgcag
     5101 tgctctacca gactgagcca cactccgact tggagggcgg cttatagagg cgcggtcgcc
     5161 ccctcgcaag ggtcaattat ggcgctcgaa aaagaagttc aaagcgacgt tgcatccatc
     5221 cgactcctgc gctatctcca ccgcctcgcc gagccccacg gggttttgcc gttcctgctg
     5281 gggaatggtg taatggtaac acagcggttt ttggtaccgt cgttctaggt tcgagtccta
     5341 gttccccagc cacttctcct tttctgcgaa gaacgcctgg cggctgctgc cgccttgcgg
     5401 cgccatgtcc gccgtgggcg gtctttgccc gcggggccgc gcgctcgggt atcgattctc
     5461 aatgtcgaga gttgagtctc tggtctagct gagaccttcg gtcgaacttt gttacatttg
     5521 accaggccag ccctgcatga atccgagcgt gatcgcgggt tacgtaccaa cggtctttat
     5581 ttggcggtcc gaaacagaaa agatcggtcg aagaatcgat ctgcgggttc aattattcca
     5641 gtcagtactt gtattccgac gtaaggggtg tatttcgaat taaagcctga ttggagacgt
     5701 aatcatggaa gacaaagcct cgctcatcga gctgactgcg gaaatcgtgg ccaactatgt
     5761 cgcgaataac agcacgccgg tgtccgatct tccggctctg attcgcgcca cgcatgacgc
     5821 tcttgcgggt attggttcgc cagaagcgcc cgtcgtcgaa gccgttacga agcctactgc
     5881 ggcgcagatt cgcaagagca tcacgcccga cgctctgatc agctttgaag acggcaagcc
     5941 ttacaagacg ctgaagcgtc acctgaccac gcacggcatg accgtcgccg aatacaaggc
     6001 caagtggggc ctgcccaacg actatccgac cacggcgccc gcctatagtg aagcccgctc
     6061 ggccatggcc aaggctctgg gtctgggtca gggcggccgc aaggccaaga gcccggccaa
     6121 ggcgccgcgc gctcgcaagg cctaggtttt tcgacatctt tcgaagaggg gctggccgcg
     6181 aggctggccc ctttttgatt cagcctcagg cggaagcacg ccccgcttcc ggcgcgggag
     6241 cggcgaaggt tcgggtcagg atctcgacca gttgcgagac ctcgatcggc ttggcgacga
     6301 tggcgtccat accggcctgg atatagagcg accgctcgtg ggtcagcacg ctggccgtga
     6361 ccgcgatgat cggggtgcgc gctcggccgg tcgaaccctc gcgagcgcgg atcgcccggg
     6421 tcgcttccac gccgtccatg ccgggcatgt ggatgtccat caggatggcg tcccagcggt
     6481 ggccttccca catcgccagg gcgtcgcggc catcgaccgc gaactggccg ctgacgccca
     6541 gatggctcag cagggtcagc aggacggtgc gattggtgtc gttgtcgtcg accaccagga
     6601 tcctgggcgg ggcctgttca tcgactggcg ccgcgatcag gggcgccgcg gcgctggcga
     6661 ccgcctcgac ctcgcgcatg gcgaccgaga acctgaagca cgtgccaccg ccggggaggg
     6721 cggaaaagtc gatctgcccg cccatcaggc cgaccagttc cttgcagatc gccaggccca
     6781 ggccagcgcc gcctgcgcgg cgggtgctgg aactgtccaa ttgggcgaac ttgccaaaga
     6841 ccaggtcgcg ctggctttcg ggaatgccgc cgccagtgtc ctcgaccgtg aaaatcaggg
     6901 tcgtggtgtc gccggcaacg ctgacccgga cctcgccgat gtccgtgaac tttatggcgt
     6961 tggagatcag gttgctcagc acctgacgca ggcgctcggg atcgcccagg cgccagccgg
     7021 cggccgaccg cgcgatatcg agcgaaaagg tcagcccctt ttcgcgggcc agtccgccat
     7081 agagctgctg cagggtttcc agcacgccct ccagggagaa cggcgtgggg atgatctcca
     7141 tccgcccggc ctcgatcctg gaaatgtcga gaatggcgtt gatcacgccc agcaaggaat
     7201 ggcccgagcg ggtgatgacg ctcaggcgct gcttctgttc cgcatccagc gcgccggcct
     7261 ccatgatctg ggccatgccc agcacgccgt tcaggtggcc aggaagtctg tcttggcgcg
     7321 gttggcggcc tgggcggcca gggccagggc ctcggccttg gcctgcagcc ggcggttctc
     7381 ctgcagttcg cgctgcagat cggcgttcag cgcgtcgctg gccaggcggg ctcggatttc
     7441 ccgtcccacc acgccgcgca tcagggtcga catgcccagc gccgcgaagg cgccggccag
     7501 gcccat
//
LOCUS       DAOMEO010000013         7371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1467_length_7371_cov_2.414844, whole genome shotgun sequence.
ACCESSION   DAOMEO010000013 DAOMEO010000000
VERSION     DAOMEO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1467_length_7371_cov_2.414844"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLH31_00610"
     CDS             <1..712
                     /locus_tag="PLH31_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR87785.1"
                     /translation="VVLTGVDVTSWGTDLPGEPTLGQLVGRILKLVPELPRLRLSSID
                     AAEIDPDLFKLLETEPRLMPYLHLSLQAGDDMILKRMKRRHSRADALKLVSEVRRVRP
                     DTAFGADLIAGFPTESDEAFENTLKLVEEAGLAFLHVFPYSARPGTPAARMPPVKGPV
                     IKDRARRLREAGQAGLERHLQRQVGRVLSGLVERDGVARAEDFTEIAFAGEASSGQIL
                     AFRVTGHDGARVIAEMAA"
     gene            786..1079
                     /locus_tag="PLH31_00615"
     CDS             786..1079
                     /locus_tag="PLH31_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HQR87786.1"
                     /translation="MNVVTKKLLQAFWTTHPGAKGPLSAWYDHAKAAAWRTPQDIKND
                     FRSVDFVGDNRVIFDIGGNNYRLVVRVSYTFKQVLVKFVGTHADYDKIDPETV"
     gene            1079..1450
                     /locus_tag="PLH31_00620"
     CDS             1079..1450
                     /locus_tag="PLH31_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR87787.1"
                     /translation="MARQISIIRDEAGYASAMAEYEAFFDKEPVLGSESGDRFELLGL
                     LLAKYEEEHFPMPKTSSVEAIRFAMDRRGLGQSDLAELLGSRSRASEILSGRRELTLP
                     QIRLLSKAWGIPVQALIVEAA"
     gene            complement(1455..2102)
                     /locus_tag="PLH31_00625"
     CDS             complement(1455..2102)
                     /locus_tag="PLH31_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HQR87788.1"
                     /translation="MDPLLDPLSTALFWLDYAAAAVWGATGALAAARRKHDIITFGFF
                     AAITGVGGGTLRDLLIGAPVFWVQRPGYILACLAAATVVWALGKRGWRFRALLWLDAL
                     GMAAFSVVGAAKALSLGVHPFTAVVMGVLTTAFGGVIRDVLAEEPNLLLRREIYITAA
                     LLGASVFTVLDLLGLGFWPAGIAGFAAAFGLRAAAIKLGWSLPGFVGGEDKAADE"
     gene            2194..2722
                     /locus_tag="PLH31_00630"
                     /pseudo
     CDS             2194..2722
                     /locus_tag="PLH31_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
     assembly_gap    2723..2732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2733..2993
                     /locus_tag="PLH31_00635"
     CDS             <2733..2993
                     /locus_tag="PLH31_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQR87789.1"
                     /translation="PHETLLVLDATVGRNALAQEKIFGNQVGVSGIVMTKLDGTARGG
                     VLVPVARASDSPIKLIGVGEGVEDLQPFDARAFSRSLVGLED"
     gene            2997..3602
                     /locus_tag="PLH31_00640"
     CDS             2997..3602
                     /locus_tag="PLH31_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HQR87790.1"
                     /translation="MDEQKTKKKNAAWVRTAVDYGAPIAFAVSYFTTRNFQTATWVLV
                     AASAVALIVGYAVERRVALLPLFSGVMALIFGALGLVFHSDVFVKIKVTVVNGALAAF
                     MIGGALMNRAPLKALMGEAMQLPDVAWRTLTLRYGAYFASAAVLNEIVRLTQDTDNWV
                     KFRVALLPLAIIFSLTQVPFMMKHMAKGEEPAAPEPPDAGF"
     gene            3706..4260
                     /locus_tag="PLH31_00645"
     CDS             3706..4260
                     /locus_tag="PLH31_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQR87791.1"
                     /translation="MSKTKVEKSGGLLERSPSHLLHRVLQLALDIYAEECGAAGVTQR
                     QFAVLSAVAQNEGVTQTALVKATGIDRSTLADMVARMIAKGHLERQRSDQDARANSVR
                     LSDSGRAMLEEIAPMVAMADARIMALLKPGKREGFLDLLADMAGADLHPAVTAEAPKA
                     KKLKADKPKKDKKLKKDKAAKKAA"
     gene            complement(4266..4535)
                     /locus_tag="PLH31_00650"
     CDS             complement(4266..4535)
                     /locus_tag="PLH31_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87792.1"
                     /translation="MSTIRPAGWPSAPAQPASTSPSGGAATRSAFFQAALGDAAPTRP
                     TQAAAAVQMQTQAQPQRQAPARPFVQPETQPQKVLRPGSLLNIVV"
     gene            complement(4634..5149)
                     /locus_tag="PLH31_00655"
     CDS             complement(4634..5149)
                     /locus_tag="PLH31_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HQR87793.1"
                     /translation="MKRWLAFAPLIVLALLAVLFAGYALKRDPNVQPQAMVGKPMPAL
                     TLPELSTGAATSLASAAQGGPVLINFFASWCAPCEIEHPQLMALSAQGVKVIGIAYKD
                     APPNTQAFLTRLGDPFTARLVDRDGAAGLEFGVTGVPETYLVGSDGVILAKHTGPLTP
                     EAAEDLLAKAR"
     gene            complement(5146..5304)
                     /gene="ccmD"
                     /locus_tag="PLH31_00660"
     CDS             complement(5146..5304)
                     /gene="ccmD"
                     /locus_tag="PLH31_00660"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HQR87794.1"
                     /translation="MDFDAGKYAIYVWPAFGITVSAFAWMIVDSLARARRWKAELARL
                     QAELDARP"
     gene            complement(5306..6043)
                     /gene="ccmC"
                     /locus_tag="PLH31_00665"
     CDS             complement(5306..6043)
                     /gene="ccmC"
                     /locus_tag="PLH31_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HQR87795.1"
                     /translation="MDARHPFDFLTNPERFMAFSRWAAPMFGAIAAMLAAVGLYLTFS
                     VPEDYQQGDTVRMMFIHVPAAIMSMVVYLFLGISSLLALIFRHALADAAARACAPLGA
                     VFTALALITGSLWGKPMWGTWWQWDARMTSVLVLFLFYLGYLALRGALEDEQKAARAA
                     AILALVGLINLPVVKFSVDWWNTLHQGSSTLFAQPEDRLPAVYLWPWALMTLAYLSAF
                     GSLWLVRIRALVWRRKARALALRMAEG"
     gene            complement(6093..6725)
                     /locus_tag="PLH31_00670"
     CDS             complement(6093..6725)
                     /locus_tag="PLH31_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HQR87796.1"
                     /translation="MHKSTRRLALAATAVLAIAASGGAALADMQPPPPPPPAPPAPPM
                     PPLPPPMPFMDAMRMAGHPLAMMSHGKPADPEAHARHLRDVLQLRPDQDSALKAYVEA
                     TAPQKMADAMMWDSKDDGDDKPLTTPQRLDRQAAHMARMAETFQKRASATKAFYAALS
                     PSQQKAFDALGPHAGGPRMRIQHLDIRHNGPPMGEHRDERKIIMKRQAGR"
     gene            complement(6836..>6972)
                     /locus_tag="PLH31_00675"
     CDS             complement(6836..>6972)
                     /locus_tag="PLH31_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQR87797.1"
                     /translation="AGALDALNSGLAWTPGLYLMLAYCAAAVALSPLAMAAACRNALD
                     "
     assembly_gap    6973..6982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6983..>7371)
                     /locus_tag="PLH31_00680"
     CDS             complement(<6983..>7371)
                     /locus_tag="PLH31_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQR87798.1"
                     /translation="AAVAPGIAWLALALSALLSLERLFERDFEDGALDLLTLGPASLE
                     AVAVAKCLAQWLASGAPLALAAPIAALALGADPKIIPLLVLCGLFGGLAFAFLGGLGA
                     SLALGSRRGGLLVAVIVLPLYVPPVIF"
BASE COUNT         1271 a         2464 c         2526 g         1090 t
ORIGIN      
        1 ggtggtgctg accggtgtcg acgtcacctc gtggggaacc gacctgccgg gcgagccgac
       61 tctgggccag ctggtgggcc ggattctcaa gctggtcccg gagctccccc gcctgcgcct
      121 gtcgtcgatc gatgcggccg agatcgatcc cgacctcttc aagctgctgg aaaccgagcc
      181 gcgcctgatg ccctatctgc atctgagcct ccaggccggc gacgacatga tcctcaagcg
      241 gatgaagcgc cgccacagtc gcgccgacgc cctgaaactg gtgtccgagg tgcgccgcgt
      301 acgccccgac accgcgtttg gcgccgacct gatcgccggc ttcccgaccg agagcgacga
      361 ggccttcgaa aacaccctga agctggtcga ggaggcgggt ctggccttcc tgcacgtctt
      421 cccctacagc gcccgccccg gcacgcccgc agcaaggatg ccgccggtca agggcccggt
      481 gatcaaggac cgcgcccgcc gcctgcgtga ggccggtcag gcgggtctgg aacgtcacct
      541 gcagcgtcag gtcggccgtg tgctgtcggg ccttgtcgag cgcgatggcg tggcccgcgc
      601 cgaggacttc accgagatcg cctttgcggg cgaggcctcc tccggccaga tcctggcctt
      661 ccgcgtcacc ggccacgacg gcgcgcgggt gatcgccgag atggcggctt aagcccgaag
      721 gcggcccgtc gggcgggaaa atctgggcgc ttgttcccaa atcgggaaca tgctatggtc
      781 tcgccgtgaa cgtcgtgacg aagaagctct tgcaggcctt ctggaccacc caccccggcg
      841 ccaaggggcc gttgagcgca tggtacgatc acgccaaggc cgccgcgtgg cgaacgccgc
      901 aggacatcaa gaatgacttc cgcagcgtcg atttcgtggg cgacaaccgg gtcattttcg
      961 atatcggcgg caacaactat cgcttggtgg tgcgggtttc ctacaccttc aaacaggttc
     1021 tggtgaagtt cgtcggcacg cacgccgact atgacaagat cgatccggag acggtgtaat
     1081 ggcgcgccag atcagcatca tccgtgacga ggccggttac gcgtcggcca tggcggagta
     1141 tgaggccttt ttcgacaaag aacccgtcct cggcagcgaa agcggtgatc ggtttgagct
     1201 cctcggcctg cttttggcca agtatgagga ggagcacttc cccatgccca agaccagctc
     1261 ggtggaagcg atccgcttcg ccatggatcg acgagggctg gggcagtcag accttgctga
     1321 actgctgggc tcgcggtctc gcgcctctga aattctgagt ggccgccggg aactgaccct
     1381 gccgcagatt cggctgctgt cgaaggcctg gggcatcccg gtccaggctc tgatcgtcga
     1441 ggccgcctga gcctctattc gtccgccgcc ttgtcctcgc cgcccacaaa ccccggcagg
     1501 ctccagccca gcttgatcgc cgccgcgcgc aggccgaagg ccgccgcaaa cccagctatg
     1561 cccgccggcc agaatcccag gcccagcaga tccagcaccg tgaacaccga cgcgcccagc
     1621 agggcggcgg tgatgtagat ttcccggcgc agcagcaggt tgggctcttc ggccagcacg
     1681 tcgcggatca cgccgccaaa agcggtggtc agcacgccca tcaccacggc ggtgaaggga
     1741 tgcaccccca ggctcagggc cttggcggcg ccgaccaccg agaatgcggc catgcccagg
     1801 gcgtccagcc acagcagggc ccggaaccgc cagccgcgct tgcccagggc ccagaccacg
     1861 gtggcggcgg ccaggcacgc caggatgtag ccgggccgct gcacccagaa caccggcgcg
     1921 ccgatcagca ggtcgcgcag ggtgccgccg cccacgccgg tgatcgccgc gaagaaaccg
     1981 aaggtgatga tgtcgtgctt gcgccgcgcg gcggccagcg ctccggtcgc gccccacacg
     2041 gcggcggcgg cgtaatccag ccagaacagg gcggtcgata acgggtcgag aagcgggtcc
     2101 atggccgttc atgccacggc caggtcgccg gcggaaacca tgccccggta atatctgatt
     2161 tacactttcg cccgttcccc taaaggagcg ctgatgagcg acaagcccga gcagaaacaa
     2221 ggctggttcc agcgcctgac ctccggcctg gcccggtcgt cccaggtgat gaccgagcag
     2281 gtcacgggga ccttcaccaa gaagcccctc gaccaggaac agctggacgc cctggaagag
     2341 atgctgatcg aggccgatct gggaccccag atcgccgccc gcatcaccga cgcctttggc
     2401 aaggcgcggt ttggcaagtc gtccaccgat atcgaggtca aggaagccct ggccgaactg
     2461 gtggccgccg agctggccga ccgcgagggg cgctttgatc cgctgtcggg gccgcgcccc
     2521 tatgtggtgc tgttcatcgg tgtcaacggc tcgggcaaga ccaccacgct gggcaagatc
     2581 gcctcggacc tgaccgaaaa gggcgcccgc gtcctgatcg ccgccggcga caccttccgc
     2641 gccgccgccg tcgagcagct gaaggtctgg gccgaccggg ccggggccga tcagcgccgg
     2701 cgcggtgccg cttctgggcc agnnnnnnnn nnccgcacga gaccctgctg gtgctggacg
     2761 ccacggtcgg ccgcaacgcc ctggcccagg agaagatctt cggcaaccag gtcggcgtgt
     2821 cggggattgt gatgaccaag ctggacggca ccgcgcgcgg cggggtgctg gtgccggtgg
     2881 cccgggccag cgacagtccg atcaagctga tcggcgtggg cgagggggtc gaggatctgc
     2941 aacccttcga cgcccgcgcc ttctcgcgct cgctggtcgg gctggaggac taggccgtgg
     3001 acgaacagaa gaccaagaaa aagaacgccg cctgggtgcg caccgccgtc gactatgggg
     3061 cgccgatcgc ctttgcggtc tcatatttca ccacccggaa tttccagacc gccacctggg
     3121 tgctggtcgc cgcctcggcc gtagccttga tcgtcggcta cgccgtcgag cgccgcgtgg
     3181 ccctgctgcc gctgttctcc ggcgtcatgg ccctgatctt tggggccctg ggcctggtgt
     3241 tccacagcga cgtcttcgtg aagatcaagg tgacggtggt gaacggcgcg ctggcggcct
     3301 tcatgatcgg cggcgccctg atgaaccgcg cgccgctgaa ggcgctgatg ggcgaggcga
     3361 tgcagctgcc tgacgtcgcc tggcgcaccc tgaccctgcg ctacggcgcc tatttcgcct
     3421 cggccgccgt gctcaacgag atcgtccgcc tcacccagga taccgacaac tgggtgaagt
     3481 tccgcgtcgc cctgctgccg ctggcgatca tcttctccct cacccaggtg cccttcatga
     3541 tgaagcacat ggccaagggc gaggagcccg ccgcgccaga accgccggac gcgggcttct
     3601 aggcggtctg cggacgggtt ccgccgttca cggattacct gtcgtcaaat cgacgtgtaa
     3661 cgatgatatt gcctgtccgt cggctgaaaa gacgaaggcg aactgatgtc caagaccaag
     3721 gtcgagaaaa gcggtggcct gctggagcgc tcgcccagcc acctgctgca ccgggttctg
     3781 caactggccc tcgacatcta tgccgaagag tgcggcgcgg ccggggtcac ccagcgccag
     3841 tttgcggtgc tttcggccgt ggcccagaac gagggcgtca cccagacggc cctggtcaag
     3901 gccaccggca tcgaccgctc caccctggcc gacatggtcg cccggatgat cgccaagggc
     3961 catcttgagc gccagcgctc cgaccaggac gcccgcgcca acagcgtgcg cctgtccgac
     4021 agcggccggg ccatgctgga agaaatcgcc ccgatggtcg ccatggccga cgcccggatc
     4081 atggccctgc tgaagccggg caagcgcgaa ggctttcttg atctgctggc cgacatggct
     4141 ggcgcggacc tgcatccggc ggtcaccgcc gaggcgccca aggccaagaa gctcaaggcc
     4201 gacaagccga agaaagacaa gaagctcaag aaggacaagg ccgccaagaa ggcggcctga
     4261 ggcttctaaa ccacgatatt cagcagcgag cccgggcgca ggaccttctg cggctgggtt
     4321 tccggctgca cgaacggccg ggcgggagcc tggcgttgcg gctgggcctg ggtctgcatc
     4381 tgcaccgcgg cggcggcctg ggtcggacgc gtgggcgcgg cgtcgccgag ggccgcctgg
     4441 aagaacgccg aacgcgtggc cgcgccgccc gacggtgagg tcgaggcggg ttgcgcgggg
     4501 gctgaaggcc agccagcggg acggatagtg ctcatgactc gaagtctaac atggttaact
     4561 gaaagtctgc gagcatggtc aacgtcgggt taacgcaaaa gcgccgcgac gtgtcagcgc
     4621 accttcagtc ggtctatcgc gccttggcca gcaggtcctc ggccgcctcg ggggtcagcg
     4681 gaccagtgtg cttggccagg atcacgccgt ccgagccgac cagataggtt tccggcacgc
     4741 ccgtgacgcc aaattccagc cccgcagccc catcgcgatc aaccagccgg gccgtgaagg
     4801 gatcgccaag gcgggtcagg aaggcctggg tgttgggcgg ggcgtccttg taggcgatac
     4861 cgatcacctt cacgccctgg gcgctcagcg ccatcagctg cggatgctcg atctcgcagg
     4921 gggcgcacca ggaggcgaag aagttgatca gcaccgggcc gccctgagca gcgctcgcca
     4981 aagaggtcgc cgcgccggtc gagagctcgg gcagggtcag ggccggcatc ggcttgccga
     5041 ccatggcctg gggctggaca ttggggtcgc gcttcagggc gtagccggcg aacagcaccg
     5101 ccagcagggc cagcacgatc agcggcgcga aggccagcca gcgcttcatg ggcgcgcgtc
     5161 cagctcggct tgcaggcgcg ccagctcggc cttccagcgg cgggcccgag ccaggctgtc
     5221 gacgatcatc caggcaaagg cgctgaccgt gatcccgaag gccggccaga catagatcgc
     5281 atattttccg gcgtcgaaat ccatctcaac cttccgccat gcgcagggcc agagctcggg
     5341 ccttgcgccg ccagaccagg gcgcggatcc gcaccagcca cagcgatccg aaggccgaca
     5401 gataggccag ggtcatcagg gcccagggcc acagatagac ggccggcagc cggtcttccg
     5461 gctgggcgaa cagggtcgag ctgccctgat gcagggtgtt ccaccagtcg accgagaact
     5521 tgaccaccgg caggttgatc aggccgacca gggccaggat cgcggcggcg cgcgcggcct
     5581 tctgttcgtc ctccagcgcg ccgcgcaggg ccagatagcc gagatagaac aggaacagca
     5641 ccaggaccga ggtcatccgc gcgtcccact gccaccaggt cccccacatc ggcttgcccc
     5701 acagcgagcc ggtgatcagg gccagggccg tgaacaccgc gcccagcggc gcgcaggcgc
     5761 gggcggcggc gtcggccaga gcgtggcgga agatcagggc cagcaggctg gaaatgccca
     5821 ggaacagata gacgaccatg ctcatgatcg ccgccggcac gtggatgaac atcatccgca
     5881 ccgtgtcgcc ctgctggtag tcctcgggga ccgagaaggt caggtacagc ccgactgcgg
     5941 ccagcatggc ggcgatggcg ccgaacatcg gcgcggccca ccgcgagaag gccatgaacc
     6001 gctcggggtt ggtcaggaaa tcaaacgggt gtcgggcgtc catggattgc tgattagagc
     6061 cctcgatgaa cgcccgcaat gcgtatcgac ccttatcggc cggcctggcg cttcatgatg
     6121 atcttgcgct cgtccctgtg ttcgcccatc ggcgggccgt tgtggcggat gtccaggtgc
     6181 tggatccgca tgcgcgggcc gccagcgtgc gggcccaggg cgtcgaaggc cttctgctgg
     6241 ctgggcgaca gggccgcata gaaggccttg gtcgccgagg cgcgcttctg gaaggtctcg
     6301 gccatcctgg ccatgtgggc ggcctgccgg tcgaggcgct ggggcgtggt caaaggcttg
     6361 tcgtcgccgt cgtccttgct gtcccacatc atggcgtcag ccatcttctg cggcgctgtg
     6421 gcctcgacat aggccttcag ggcgctgtcc tggtcggggc gcagctgcag cacgtcgcgc
     6481 aggtgcctgg catgggcttc cggatcggcg ggcttgccgt ggctcatcat cgccaagggg
     6541 tggccggcca tcctcatcgc gtccatgaac ggcatgggcg gcggcagcgg gggcatgggc
     6601 ggcgccggcg gggcgggggg cggaggcggc ggaggttgca tatcggccag ggcggccccg
     6661 ccgctggctg cgatggcgag aacggcggtg gccgccaaag ccaggcgacg cgtggattta
     6721 tgcatgatgg tttctccctc ggaaccagat ctggcgagag cttggccatc ggcttatggc
     6781 gcgaaaatgt caggttagat gaacaatgtt acaggattgg cgaaggtggg gagcctcagt
     6841 ccagcgcgtt gcggcaggcc gccgccatgg ccagggggct cagcgccacg gccgctgcgc
     6901 aataggccag catcagatag aggcccggcg tccaggccag gccgctgttc agggcgtcta
     6961 aagccccggc ccnnnnnnnn nngaagatca ccggcggcac atacagcggc agcacgatca
     7021 ccgccaccag aaggccgccg cgccggctgc ccagggccag gctggcgccc agtccgccca
     7081 gaaaggcgaa ggccaggccg ccgaacaggc cgcaaagcac cagcagggga atgatcttcg
     7141 gatcagcgcc cagggccagg gccgcaatgg gcgccgccag ggccaggggc gcgcccgagg
     7201 ccagccactg ggccaggcac ttagcgacgg ccacggcctc cagcgacgcg gggcccaggg
     7261 tcagcaggtc cagggcgccg tcctcgaagt cgcgctcgaa caatcgctcc agcgacaaca
     7321 gggccgagag cgccagggcc agccaggcga tgcccggcgc cacggcggcc a
//
LOCUS       DAOMEO010000014         7268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1487_length_7268_cov_3.112020, whole genome shotgun sequence.
ACCESSION   DAOMEO010000014 DAOMEO010000000
VERSION     DAOMEO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_7268_cov_3.112020"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLH31_00685"
     CDS             complement(<1..334)
                     /locus_tag="PLH31_00685"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQR87799.1"
                     /translation="MAQKSSPLRILVTGGAGFVGSHLCDRLIARGDHVVCLDNLFTGS
                     KDNIAHLMDHPNFEFVNHDVNHPIFIPVDQIYNLACPASPIHYQYDPVMTVRTNVMGA
                     INMLDLAKR"
     gene            complement(339..1205)
                     /locus_tag="PLH31_00690"
     CDS             complement(339..1205)
                     /locus_tag="PLH31_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87800.1"
                     /translation="MLVHYFKPEANPRHTSVDESRRDNRTNAIETVIRGYRGLFGSSA
                     KLRFADRSLDITPGPMDLDIRVLTAPGCSLVSQEFRRTHGVWETTAMPNNPRMLGFFA
                     YQIFAKLAGDYDWFIFSEDDLLVRDPLLFDKLAWFNEAFGDSRVLLPNRLEWNPSAKR
                     LKTYVDGDISAGTLANMNRHLPDEDVLKARPLHAEHVFQRARNPHSGFFAITKTQLAH
                     WMAQPHWLDYDVSFVSPLESAATLGLTKTFPIYKPQGNSSAFLEVEHLDDRFSKMPWP
                     IHVPAVTASPLE"
     gene            1345..2292
                     /locus_tag="PLH31_00695"
     CDS             1345..2292
                     /locus_tag="PLH31_00695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR87801.1"
                     /translation="MPLMFLMSVLALADTSPRPFGLPLDCDLGRTCEIQNYVDRDPGP
                     GAKDYRCSSRTYEAHSGTDFRILDMRAQKAGVAVLAAADGKVLRIRDGVADVSVREAG
                     AVLGQECGNGLVIEHADGLSTQYCHMAKGSLAVKPGMTVKAGAKLGLVGLSGNTEYPH
                     LHFTVRRGGAVIDPFAPDATGGPNQCGGGKSLWRASPPYKAGAVLNVGFAAEPVSLAA
                     VEAGVVPPTGSSNALIAYVRAIGLQAGDQQSLSLHAPNGAILAKTDTPPLARDQAQRL
                     LYVGKPRPSTRWGKGRYEAVYRVVRKGAPVIERRWTIAL"
     gene            complement(2533..4215)
                     /locus_tag="PLH31_00700"
     CDS             complement(2533..4215)
                     /locus_tag="PLH31_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR87802.1"
                     /translation="MTNVALYLNPEAFNTKGPALMGRQSAGEGFLRGYLRHARSEDIH
                     FWNVADRPVAELDAFVQAIGAIDRPVKWIARHDRLGLGDAGSVHMASPRLAREAWARR
                     SIRASNRYGISGITHTTASSDVTDMLADMMTAPLEPWDFLICTSQAVRASVDLQLEAV
                     RDDLQIRLGATRLPTPELITIPLGVNVDDFSVSDASRQNWRRELDIPDDAVVVLYVGR
                     FNVRAKMNPAPMAMALQRAVELTGKKIAWVQAGWAGSEALNKTYHDETAAFCPDVIYR
                     VVDGRRADVRFSIWSVGDIFFSLSDNIQETFGLTPVEAMAAGLPNVISDWNGYRDTVR
                     HGVDGYRIATYAPRAGLGEDLAYRAASDWINYDAYVGSASQVTAVDLGEAAQAIADLV
                     ASYDLRRTMGQAAQTRARQIFDWSAIIPQYEALWDEMNTRRGRAGPEQAAPRNLARNP
                     RRLDPFHLFGGYPTEWLTATTMVALTPGQSAQSLGPLIRSSLTNHGGALLPSKLEMDR
                     IIECLSRTPKLRVADLTNLFPVERRLFVERGLLWMAKYQIVTILPRSDHIAV"
     gene            complement(4352..>4908)
                     /locus_tag="PLH31_00705"
     CDS             complement(4352..>4908)
                     /locus_tag="PLH31_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87803.1"
                     /translation="DTISGGAGDDTLTGGALADTILGGADADTITGDAGADTLSGGAG
                     ADTIVAGADADTVNGGTGTDTLTGDAGNDRFVFALGDSGTSVGAIDTITDFVAGSAAD
                     TIDITETGTIALTVGTAASTYAIALATAQGLVATPGANADVVAIQVNADTYVFFDANN
                     DGTIEDAVLLTAVTAANLVVGNFI"
     assembly_gap    4909..4918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4919..6886)
                     /locus_tag="PLH31_00710"
     CDS             complement(<4919..6886)
                     /locus_tag="PLH31_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQR87804.1"
                     /translation="MANLDFNTATQADVDAWAPGASDDLVFATGTATQLTYTITAAVP
                     AQGATPAVEEKITLTLGAKSLTFFTSQIDGAGSVSFTDGSVFAYGTTGADGLAGGALA
                     DLLLGANGDDIITGANGADMIFGGEGADSLEGGDGDDTVQGNQGNDTLVGGAGSDTLY
                     GGQNTDTIVGGAGNDYINGNLGTDSISGGEGADTVMGGNQGDTLDGGNGDDYLSGDLE
                     ADSIVGDNGADTLMGGQGADTLFGANGDDYLDGGTESSTIDAGEGNDTVVSTSNGDDS
                     ILAGLGNDNVASGNGNDTIAASNGDDVVNGGAGNDTIDGGFDNDSVQGALGNDSILGG
                     NGSDTLLGGQGNDTIDGQDQNDYVHGNLGDDVVNGGAGNDTLLGGQGLDTMDGGTGND
                     YVTGDLGNDSIDGAAGNDTILGADGDDVVFGVGAGSDFVYGGAGNDTLDGGTEGDTVN
                     GEAGDDYVEGGAGSDFVNGGEGNDSVSGGADADTVNGDAGNDTIDGGDGSDLVFGGEG
                     NDRILATVGGIDTLDGGAGNDTFVVTGGTASIIGGEGADSVLGGGGADTINGGDGNDV
                     LTSGAAAVTINGGEGTDTITGGAGGDILGGDNGADTINGGGGADTINGGDGNDTITVI
                     TDAATVNGGEGVDIIAGGAGADKLNGDNGSDS"
BASE COUNT         1397 a         2208 c         2205 g         1448 t
ORIGIN      
        1 tgcgcttggc caggtccagc atgttgatcg cgcccatcac attggtgcgc acggtcatga
       61 ccgggtcgta ctggtagtgg atcggcgagg ccgggcaggc caggttgtag atctggtcga
      121 ccggaatgaa gatcgggtgg ttgacgtcat ggttcacgaa ctcgaagttc gggtgatcca
      181 tcaggtgggc aatgttgtcc ttgctgccgg tgaacagatt gtccaggcac accacgtgat
      241 cgccccgcgc gatcaaacgg tcgcacaggt gagagccgac gaagccggct ccgccagtga
      301 ccagaatacg caggggagaa gatttctggg ccaaagcttt attccaaagg agaggctgtc
      361 acggccggga cgtggatcgg ccatggcatt ttcgagaagc gatcatcaag gtgctcgacc
      421 tcaaggaagg ccgacgaatt cccttgtggc ttatagatcg ggaaagtctt ggtcagcccc
      481 aaagtcgcgg cgctttccaa cggcgaaacg aagctgacgt catagtccag ccagtgcggc
      541 tgggccatcc agtgcgcaag ctgggtcttg gtgatggcaa agaaacctga atgcgggttc
      601 cgggctcgct ggaacacatg ctcagcatgc agggggcgcg ctttgagaac atcctcatcg
      661 ggcaagtggc ggttcatatt cgccaacgtg cccgcgctga tgtccccatc cacataggtc
      721 ttcaggcgct tggccgatgg attccactca agccgattgg gcaagagaac tcgactgtcg
      781 ccgaacgctt cgttgaacca agccagcttg tcgaagagca gcgggtcgcg aaccaaaagg
      841 tcatcttcgc tgaagatgaa ccaatcatag tcgcctgcca gcttggcgaa tatttgatag
      901 gcgaagaacc ccaacatccg gggattgttt ggcatggcgg tcgtttccca aaccccgtgg
      961 gttcgtcgaa actcctggct caccaggctg caacccgggg ccgtcaggac cctgatgtcg
     1021 agatccatcg ggcctggtgt gatgtcgagg cttcggtcgg caaagcgaag tttcgccgaa
     1081 gagccaaaca agccacgata gcctcggatt accgtctcaa tggcgttcgt acggttatcc
     1141 cgtcgagact cgtcaacgga cgtatgccgc gggttggcct cgggtttgaa atagtggacc
     1201 agcaccacga gagcccgcat cggtgatcac ctctttaggg cctccagcct gcctggcatc
     1261 ccacgccgcc ttgcctagcg gctccacaag cgtcgctcaa gcaccgcctc gacggcgcct
     1321 tgtttctatg gctgccttgg gcccatgccc ttgatgtttc tgatgtctgt tctggccttg
     1381 gctgatacct cgccacgtcc tttcggcttg ccgcttgatt gcgacctcgg gcgaacttgc
     1441 gagatacaga actatgtgga tcgcgatcct gggcccggag ccaaggacta ccgttgtagc
     1501 tcgcggacct atgaggcgca ttcgggcacg gatttcagga tcctggacat gcgggcgcag
     1561 aaggccggcg tagcggtctt ggcggccgca gacggtaagg tcctgcgaat ccgcgatggc
     1621 gttgccgatg tctcagtgcg cgaagccgga gccgtgctgg gccaggagtg cggcaacggc
     1681 ttggtcattg agcatgctga cggactgtcc acacagtact gccatatggc aaagggcagc
     1741 ctggccgtaa agccaggtat gacggtgaag gctggggcca agctgggcct cgtcggcctt
     1801 tcgggaaata ccgaatatcc tcatctgcac ttcacggttc gccgaggggg cgctgtgatt
     1861 gatccgttcg cgcctgacgc gactggcggg ccaaaccaat gtggaggcgg caaaagcctt
     1921 tggcgggcgt caccgccgta caaggctggt gcggtcctaa atgtcgggtt tgccgcagaa
     1981 cccgtcagcc tggccgcagt ggaggcgggc gtcgttccgc caacaggttc ctctaacgct
     2041 ctgatcgcct atgtccgcgc gatcggactg caggctggcg accaacaaag cctcagtctt
     2101 cacgcgccca atggcgcgat acttgccaag acggacactc ccccccttgc aagagatcag
     2161 gctcaacgtc tgttgtatgt ggggaagcct cgcccctcga cgcgatgggg caagggccgc
     2221 tacgaggcag tttatcgtgt ggtgcgtaaa ggcgcgcccg tgatcgaacg acgttggacc
     2281 atagcgctat aagcctgtca ggcgagtaaa gttgagaaga gtcccgttag gccttttgtc
     2341 tgggaggggc ttatgccatt atggggcgtc agccttgttc tccagcgggc tgtgtcactc
     2401 cgaccgggct gtgaaacgcg catatatttt tcacagtccg gtcgctcgcc ttcgcgagct
     2461 agctggtttc tcttaaaatc tagattcctt gaccctggcg ggctcgattc cttctgtgtt
     2521 gaaggcgtct attcagaccg cgatatggtc tgatcgaggc aggatcgtta cgatctggta
     2581 cttcgccatc cacaacagcc cacgctctac aaagagcctg cgctccaccg gaaacaaatt
     2641 cgtcagatct gcaacgcgaa gcttgggtgt gcgcgacaga cactcaatta tccgatccat
     2701 ctccagcttg gagggcaaaa gagccccgcc gtgattggtc aagctgctgc gaatgagtgg
     2761 gccaagcgat tgtgcagact ggcccggtgt aagagcgacc atagtggtcg cggtcagcca
     2821 ctcggtgggg tagccgccaa aaagatgaaa cggatcgagg cggcgcggat tccgcgcgag
     2881 gttacgaggc gccgcctgtt ccggccctgc gcgaccccgc cgcgtattca tctcatccca
     2941 caaggcttca tattgcggaa tgatcgccga ccaatcgaaa atttgccgcg cccgcgtctg
     3001 agcggcctgc cccattgtcc ggcgaagatc gtagctggcg accaggtcgg cgatcgcctg
     3061 agcggcctcg cccaggtcaa cagccgtgac ttggctggct gagccaacat aggcgtcgta
     3121 gtttatccag tcgctcgccg cgcgatacgc caggtcttcg ccaaggccgg cgcgcggggc
     3181 gtaggtggcg atgcgatagc catcaacacc atgacgaacg gtgtcgcgat agccgttcca
     3241 gtcgctgatc acgttgggca gtcctgcggc catggcttcg accggggtca gaccaaaagt
     3301 ctcctgaata ttgtccgaca gcgagaaaaa gatgtctccc acggaccaga tggaaaaccg
     3361 aacatccgcg cggcgaccat cgaccacgcg atagatgacg tcgggacaga atgccgccgt
     3421 ttcgtcgtga taggtcttgt tcagggcttc actaccggcc caaccggcct gcacccaggc
     3481 gatcttctta ccggtcagct cgacggctcg ctgcaaggcc atcgccatgg gagcggggtt
     3541 catcttggcg cggacattga accggccgac atagagcaca acaacggcgt catccgggat
     3601 atcgagctcg cgccgccaat tctggcgact ggcgtccgat accgaaaaat cgtcgacgtt
     3661 tacgcccaag ggtatcgtga tcagctccgg cgtgggcaat cgtgttgcgc ccaagcggat
     3721 ctggagatca tcgcgaactg cttccagctg aagatcaacg ctggcccgca cggcctggga
     3781 tgtgcagatc aggaagtccc agggttccag cggcgctgtc atcatgtccg ccagcatgtc
     3841 ggtgacatcg gacgaggccg tagtgtgcgt gatcccgcta atgccgtagc gatttgaagc
     3901 ccgtatggac cgtctggccc aggcttcgcg cgccagtcga ggcgaggcca tgtgaacgga
     3961 gcccgcatcg ccgagaccca agcgatcatg tctggcgatc catttcacag gccgatcaat
     4021 ggcgccgatg gcctgaacaa aggcatccag ttcggcgacc ggtcggtcgg ccacattcca
     4081 gaaatggatg tcttcgctgc gtgcgtgtcg cagatagccg cgcaggaacc cctcccccgc
     4141 cgactgccgg cccatcaagg ccggcccctt cgtgttgaag gcttcggggt tcagatacag
     4201 cgctacattg gtcatagacc gcaattcctc cgcgtcagcc cggcacgcgg ggtctgtcgg
     4261 ttcagccaat gacgacaccg ccccccgttg tcgaggccaa aaaaaagggg gccgccccga
     4321 aggacggccc ccaatcttgg atggtctaaa cttagatgaa gtttccaacc acgaggttgg
     4381 cggcagttac ggccgtcagc aacaccgcat cttcaatcgt gccgtcgttg ttggcatcga
     4441 agaagacgta ggtatcggca ttaacctgga tggcaacaac gtccgcattc gcgccaggag
     4501 tggcaacgag gccctgggcc gtcgcaagag cgatggcata ggtggacgca gcagtaccaa
     4561 cggtcagcgc gatcgtacca gtctctgtga tatcgatcgt gtccgcagca gaaccagcaa
     4621 cgaagtcggt gatggtgtcg attgcaccga ccgaggtgcc cgagtcgccg agagcgaaca
     4681 cgaaccggtc attgccagca tcaccagtca gcgtatccgt acccgtacca ccgttgaccg
     4741 tgtcagcgtc ggcgccagca acgatcgtgt cagcgccagc accgcccgag agcgtatcag
     4801 cgccagcgtc gcccgtgatc gtgtcggcat ctgcgccgcc aaggatcgtg tccgccagag
     4861 cgccgccggt gagcgtatcg tcacccgcac cgccgctgat ggtgtcgann nnnnnnnncg
     4921 agtcggagcc gttatcgccg ttcagcttat cagcaccagc gccgccggcg atgatgtcaa
     4981 cgccttcgcc gccgttaacc gtggccgcat cagtgatcac ggtgatggtg tcgttaccgt
     5041 cgccaccgtt gatggtgtca gcaccgccgc caccgttgat ggtgtcagca ccattgtcgc
     5101 cgccgaggat gtcgccacca gcgccgccgg tgatggtgtc agtaccttcg ccgccgttga
     5161 tggtcaccgc agccgcgcca ctggtgagca cgtcgttgcc gtcgccacca ttgatggtgt
     5221 cagcgccgcc gccgcccaga accgagtcag cgccttcgcc gccgatgatg ctggccgtgc
     5281 cgccggtcac cacgaaggtg tcgttaccgg cgccgccgtc gagggtgtca ataccgccga
     5341 ccgtagccag gatgcggtca ttgccttcgc cgccgaacac caggtcgctg ccgtcgccgc
     5401 catcgatggt gtcgttgccg gcgtcgccgt tgacggtgtc agcatcagcg ccgccggaga
     5461 cggagtcgtt gccttcgccg ccattgacga agtcggagcc agcgccgccc tcgacgtagt
     5521 cgtcaccggc ttcaccgttg acggtatcgc cttcggtgcc gccgtcgagg gtgtcgttac
     5581 ccgcgccgcc ataaacgaag tcggagcccg cgcccacgcc gaagacgacg tcatcgccat
     5641 cagcgccgag gatggtgtcg ttgccagcag cgccatcgat gctgtcattg cccaggtcgc
     5701 cggtgacgta gtcgttgccg gtgccaccgt ccatggtgtc gaggccttga ccgcccagaa
     5761 gggtgtcatt gccggcgccg ccgttgacga cgtcatcgcc gagattgccg tggacgtagt
     5821 cgttctggtc ttgaccatcg atggtgtcgt tgccttgacc gcccagcagg gtgtcagagc
     5881 cattaccgcc gaggatcgag tcgttaccaa gagcgccctg gaccgagtcg ttgtcgaagc
     5941 cgccgtcgat ggtgtcgttg ccggcgccac cgttgaccac atcgtcgccg ttgctggcgg
     6001 cgatggtgtc attgccgttg cccgacgcaa cgttgtcgtt gcccaggcca gccaggatgc
     6061 tgtcatcacc gttgctggtc gaaacgacgg tgtcgttgcc ttcgccggcg tcgatggtgc
     6121 tgctttcggt gccgccgtcg agatagtcgt cgccgttagc gccaaacagg gtgtcggcgc
     6181 cttggccgcc catcagggtg tcagcgccgt tgtcgccgac gatcgagtca gcttccagat
     6241 cgcccgacag atagtcatca ccgttgccgc catccagggt atcgccctga ttgccgccca
     6301 tgacggtgtc ggcgccttcg ccacccgaga tcgagtcggt gccgaggttg ccgttgatgt
     6361 agtcattgcc agcgccgccg acgatcgtgt cggtgttttg gccgccgtag agggtgtcag
     6421 agccagcgcc gccaacgagg gtgtcgttgc cttggttgcc ctgcacggtg tcgtcgccgt
     6481 cgccgccttc aaggctgtca gcgccttcgc cgccgaagat catgtcggcg ccattagcgc
     6541 cggtgatgat gtcgtcgcca ttggcgccga gcagaaggtc agccagcgcg ccgccggcca
     6601 ggccatcagc gcccgtggtg ccataggcga acacgctgcc gtccgtgaac gacaccgaac
     6661 ccgcaccgtc gatctgcgac gtgaagaagg tcagcgactt ggcgcccagc gtcagggtga
     6721 tcttttcttc caccgcaggc gtcgcgcctt gagccggaac cgcagcagtg atcgtgtagg
     6781 tcagttgcgt tgcggtgccg gtcgcaaaaa ccaggtcatc gctcgcaccc ggcgcccagg
     6841 catctacgtc tgcctgagtt gccgtattga aatccaaatt agccatccaa gttctccatc
     6901 ttgtgtcaca ggcaataaac gccgacgccc cacccccaag acgggaacat atcggcgcaa
     6961 acccagcctt ctgccgcttt taacggaggc tgagattatt acaacccagc cggatggttg
     7021 cccaaacgcg agccaagcgc aactccgtta aggctggggc cgtagcatcc gcatcgacgc
     7081 ctcctaaaag catcagatat agatatggcg cctatgctca aagggggagc cctcgcaata
     7141 caatattttt gtgtaatttg tgataattat attatataat ttctatattt gaaaggatag
     7201 tccagttttt tcgttgaaaa tgagctgatc tagagcaaaa acttccttta gatattctct
     7261 atcttcca
//
LOCUS       DAOMEO010000015         7207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1502_length_7207_cov_2.858501, whole genome shotgun sequence.
ACCESSION   DAOMEO010000015 DAOMEO010000000
VERSION     DAOMEO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1502_length_7207_cov_2.858501"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /locus_tag="PLH31_00715"
     CDS             complement(<1..1801)
                     /locus_tag="PLH31_00715"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HQR87805.1"
                     /translation="MSTAPVPPRALRSRAWFDNPDNVDMTALYLERYLNFGLSLEELT
                     SGKPIIGIAQTGSDLSPCNRHHLVLAERVREGIRTAGGIALEFPVHPIQETGKRPTAG
                     LDRNLAYLGLVETLYGYPIDGVVLTIGCDKTTPACLMAAATVNIPAIALSVGPMLNGW
                     HKGQRTGSGTIVWKAREMLAAGEIDREGFIKLVASSAPSTGYCNTMGTATTMNSLTEA
                     LGMSLTGSAAIPAPYRDRQQNAYETGLRIVDLVRQDIKPSDILTRDAFLNAIVVNSAI
                     GGSTNAPIHLNALARHIGVQLSVDDWQIHGEEVPLLVNLQPAGEYLGEDYYHAGGVPA
                     VVNQLMTQGLIREDALTVSGQTIGQACGGAVIEDVAVIRPFHNPLVQRAGFVVMRGNL
                     FNSAIMKTSVVTAEFRDRYLSDPNDPDAFEGDAVVFDGPEDYHARIDDPAVGITERSV
                     LFMRGAGPIGYPGAAEVVNMRAPDYLLKRGISALPCIGDGRQSGTSGSPSILNASPEA
                     AAGGGLALLKSGDRVRVDLRKSRVDVLITPEEIVARRAALEAAGGYAYPESQTPWQEI
                     QRGLVGQMETGAVLEPAVKYQRIAQTKGLPRDNH"
     gene            2107..3102
                     /gene="gguC"
                     /locus_tag="PLH31_00720"
     CDS             2107..3102
                     /gene="gguC"
                     /locus_tag="PLH31_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GguC family protein"
                     /protein_id="HQR87806.1"
                     /translation="MVLRFVQLKEHGGERVLAAVNDAGLARRVEGADTLLDLANAALK
                     AGATLAEAAQASPWGDEIDIPAALAEGRVLAPVDHPDPAHLILTGTGLTHLGSAEGRD
                     KMHKAAQASDQPTDSMRMFLMGVEGGKPTEGAAGVQPEWFYKGDGSHLRAYGQPLESP
                     AFALDGGEEPEIAGIYLIGPDGTPHRLGFCLANEFSDHVTERGNYLWLAHSKLRQAAL
                     GPELLTGPLPQDVRGTSRILRDGAVLWADAFLSGEANMSHTIANLEYHHFKYGVFRRP
                     GDLHVHFFGTATLSFSHGITPRAGDIFEISAAPFHYPARNPLALAIAEPVAIRSL"
     gene            3180..4380
                     /locus_tag="PLH31_00725"
                     /pseudo
     CDS             3180..4380
                     /locus_tag="PLH31_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
     gene            complement(4438..6015)
                     /locus_tag="PLH31_00730"
     CDS             complement(4438..6015)
                     /locus_tag="PLH31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HQR87807.1"
                     /translation="MNLTGELLIGGGATRGAQGEIRGVNPATGEVLDPAFGGATLQNL
                     EAACAQAAAAFQPYRALPYETRATFLEAIAEHIEALGDDLIVRVMAESGLPRARLEGE
                     RGRTTGQLRLFASVLRDGGFLEARIDPAMPDRKPMARPDLRLRNIPLGPVAVFGASNF
                     PLAFSVAGGDTASALAAGCPVVVKAHPAHPGTSEMIGRAIQSAVAACGLHPGVFSLIH
                     DSGFEISQALVADPRIKAAGFTGSRRGGLALAAIAQGRPEPIPFYAEMSSINPVLLFP
                     AALAARGPAIARDFVGALTLGAGQFCTNPGLILALDGPELDGFLTAAEEALSPVPAAT
                     MLTPAISRVYADGVRALAQTPVVKQVAVGQEALQNQGQAALFGVSGADFLAHPHLAEE
                     VFGAASIVVRCADLAELHKVVVALEGQLTIALHMDEADHVLAADLLPDLELKAGRILV
                     NGFGTGVEVAHAMVHGGPFPATSDSRVTSVGTLAIARFLRPVSYQNLPGALLPDELKA
                     ENPLGLVRRVDGKLNLI"
     gene            6166..6513
                     /locus_tag="PLH31_00735"
     CDS             6166..6513
                     /locus_tag="PLH31_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87808.1"
                     /translation="MARNLSGKVVASGADVTALADKAWFDAHPERDFMLRDPAPLEFR
                     EPLGDAGEGFSWRVLIVRLGDGSRLRLPISLAWDLHNDHAKEQHLAVIFEQVAPEQAR
                     VLRAAALAGAPRV"
     gene            6927..7136
                     /locus_tag="PLH31_00740"
     CDS             6927..7136
                     /locus_tag="PLH31_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQR87809.1"
                     /translation="MTTGTVKWFNSTKGFGFIQPDDGGADVFVHISAVERSTLGSLNE
                     GQKLSYELERDQRSGKMSAGQLQAA"
BASE COUNT         1217 a         2383 c         2406 g         1201 t
ORIGIN      
        1 agtgattgtc ccggggcagg cctttcgtct gggcgatgcg ctggtacttg accgcaggct
       61 ccagcaccgc gccggtctcc atctgcccga cgaggccgcg ctggatctcc tgccagggcg
      121 tctggctttc cggataggcg tagccgccag ccgcctccag cgccgcgcga cgggccacga
      181 tttcctcggg ggtgatcagc acgtcgaccc gcgacttgcg caggtcgacc cgcacgcgat
      241 cgccggactt cagcagggcc agcccgccgc cggccgccgc ttcgggcgag gcgttgagga
      301 tcgagggcga gcctgaagta cccgactgtc gcccatcgcc gatgcacggc agggcgctga
      361 tcccgcgctt gagcagatag tcgggagccc gcatgttcac cacctcggcg gcgcccggat
      421 agccgatcgg gccggctccg cgcatgaaca gcacgctgcg ctcggtgatg ccgacggcgg
      481 ggtcgtcgat gcgggcgtga tagtcttcgg gcccatcgaa caccaccgcg tcgccctcga
      541 aggcgtcggg gtcattgggg tcggaaagat agcgatcgcg gaactcggct gtgaccacgc
      601 tggtcttcat gatcgccgag ttgaacaggt tgccgcgcat gaccacgaag cccgcgcgct
      661 ggaccagcgg attatggaac ggccggatca cggctacgtc ctcgataacg gccccgccgc
      721 aggcctgacc gatcgtctgg ccggaaaccg tcagggcgtc ctcgcggatc aggccctggg
      781 tcatcagctg gttgaccacg gcgggcacgc cgccggcgtg ataatagtcc tcgcccagat
      841 actctccggc gggctgcaga ttgaccagca gcggtacctc ttcgccatgg atctgccagt
      901 catcgacgct gagctggacc ccgatatggc gggccagggc gttgagatgg atcggcgcat
      961 tggtcgatcc gccgatcgcg gaattgacca caatggcgtt gaggaaggcg tcacgggtca
     1021 ggatatcgga cggcttgata tcctgacgca ccagatcaac gatccgaagg ccggtctcgt
     1081 aggcgttctg ctgtcggtcg cggtaaggcg ccggaatagc cgccgagccg gtcagcgaca
     1141 tgcccagcgc ttcggtcagc gagttcatcg tcgtggccgt gcccatggtg ttgcaatagc
     1201 cggtcgacgg cgccgagctg gccaccagct tgatgaagcc ctcccgatcg atttcgccgg
     1261 ccgccagcat ttcacgggcc ttccagacga tggtgcccga gcctgtccgc tggcccttgt
     1321 gccagccgtt cagcatggga ccaaccgaca gggcgatggc tggaatgttc accgtcgcag
     1381 cggccatcag gcaggcaggt gtggtcttgt cgcagccgat ggtcagcacg acgccatcga
     1441 tcggatagcc gtacagcgtc tcgaccagac caagataggc aaggttacgg tccagccccg
     1501 ccgtgggtcg cttgccggtt tcctggatcg ggtggacagg gaattcgaga gcgatgccgc
     1561 cagcggtgcg gataccctcg cgcacccgtt cggccaggac gaggtggtgg cgattgcacg
     1621 gcgacaggtc actgccggtc tgggcgatgc cgatgatcgg cttgcccgac gtaagttcct
     1681 ccagcgacag gccgaaattc aggtaccgct cgagatagag cgcggtcatg tccacattgt
     1741 cgggattatc gaaccaggcg cgcgaacgga gagcgcgagg cggaacgggg gcagtgctca
     1801 tgacagtcct tggatcaacg cggggcccgg gattatcgca tggtcccgcg ccgctgcgtg
     1861 attgtaatat caataaattg tctgactata ttcagcgtgc accgcggtca acatgccgga
     1921 gccatgcaag gccgcgccga gtgttcctgc gcggcgcttg ccggtcagac ctacgctgat
     1981 gggctcggcg cccgaacgca gaaaaaggga tccaccatgg gttagacccg atccgatccc
     2041 ctttgacagt cagcctcagg cggctatttg tctgaccaga ttggatgctc gggaacggtg
     2101 acggccatgg tgctgcggtt tgtgcaactg aaggaacacg gcggcgaacg cgtgctcgcg
     2161 gcggtcaatg acgccggtct ggcgcgacgc gtcgaaggcg cagacaccct gctcgatctg
     2221 gccaacgccg cgctgaaggc cggcgccacc ctggccgagg ctgctcaggc cagtccgtgg
     2281 ggcgacgaga tcgatatccc cgccgccctt gctgaaggtc gggtgctggc gccggtggac
     2341 caccccgatc cagcccatct gatcctgacc ggcacgggcc tgacgcatct tggttccgcg
     2401 gaaggtcgcg acaagatgca caaggccgcc caggcgtccg accagcccac cgactcgatg
     2461 cgcatgttcc tgatgggcgt ggaaggcgga aagcccaccg agggggccgc cggcgttcag
     2521 cccgaatggt tctacaaggg cgatggctct cacctccgcg cctatggcca gcctctggag
     2581 tcacccgcct tcgcgctgga cggcggcgag gagcccgaga tcgccgggat ctatctgatt
     2641 ggtccggacg gaacgccaca tcgcctgggc ttttgcctgg ccaacgagtt ctccgaccac
     2701 gtgaccgagc ggggcaacta tctgtggctg gcccattcca aactgcgcca ggccgcgctg
     2761 ggtcccgaac tgctgaccgg ccccctgccc caggacgtgc gcgggaccag ccggatcctg
     2821 cgcgacggcg cggtgctctg ggcggacgcc ttcctgtcgg gcgaggccaa tatgagccac
     2881 accatcgcca acctggaata ccatcacttc aagtatggcg tctttcgccg gcccggcgat
     2941 ctgcacgtgc acttcttcgg cacggcgacg ctgtcgttca gtcacggcat tacccctcgc
     3001 gctggggaca tcttcgagat ttccgccgcg ccattccact atccggcgcg caaccccctg
     3061 gccctcgcga tcgccgagcc cgtcgcgatc cgcagcttgt gacccggccg cccttcaagt
     3121 ggggccacgg cttcgaggaa ggcattgccg cgtgcggcgg ctcgtctaaa gtcctgtcca
     3181 tgcaaacgcg tctcctcccc ctcatcgccc tggctctgtg cgcctgcggc cccgctcccg
     3241 atgcgccggt tcaggacgcc tcgggcgcgc cggtcgaaac ccggcccgcc aatacgccgg
     3301 accagaagcc ggccttcgcc ggccagaccc gcgcgcccgg ccaggcgctg ggcgtcgcct
     3361 acagtgtcga gacggtggtc agcggcctgg accatccgtg gggcctggcc ttcctgccgg
     3421 acgggaccag gctggtcacc gagcgcgccg gacggctgcg cctggtcggc gccgacggca
     3481 agctgtcaat gcccgtggcg ggtctcccgg ccgtagacgc cgacggacag ggcgggttgc
     3541 tcgacgtcgc agtcgatccg aatttcgcca gcaatggcct gatctactgg acctatgccg
     3601 aggcggggac tggcggcaac ggtacggcgg cggcgcgcgg caagctggtg tccggctccg
     3661 ccccgcgagt cgaaggcgtg caggtgatct ggaggcaggc gcccaagctg aactcaagcc
     3721 tgcacttcgg atggcaagct gttcatcacc accggtgatc gctcgatcct ggaaggccga
     3781 atgcaggccc agcgtctgga cggggccata ggcaaggtta tccggatcaa cgctgacggc
     3841 tcaatccctg cggacaatcc ccatgtcggc aaggccggag cgaggccgga aatctggtcg
     3901 gcgggccacc gtaacctgca ggccgccgca ctcgacccgt ctggccggct gtggaccgtg
     3961 gagcacggcg ccaagggtgg cgatgagctg aaccgtcccg agccaggcaa ggactatggc
     4021 tggccgacca tcacctatgg cgaggagtat tctggcaggc ccatcggcga cggcgtcacc
     4081 gccaaggcgg gtctggagca gccggtctac tactgggacc cggtcatcgc gccctcgggc
     4141 atggccttct atcaggccga tctgttcccg gcctggaagg gcagcctgct ggtcggcgcg
     4201 ctgcgcggac agcacgtggc ccgtctgaca ctcaacggcg acagggtggt cggcgaggag
     4261 cgcctgttcg aagacgtcga caaacgcatc cgcgatgtcc gcgtcggccc cgacggcgcg
     4321 gtctatctgc tgaccgacga ggaagacggc aagctgctgc gcgtggttcc aaagccctag
     4381 ccgagccgcg tcccgtgaag acagcgccgg ccgtagggca cggccggcgc atcgacttca
     4441 gatcaggttg agcttgccat cgacccggcg caccagaccc agagggttct cggccttcag
     4501 ttcgtcaggc agcagcgcgc cgggcaggtt ctggtagctg accggccgca ggaagcgggc
     4561 gatggccagg gttcccacgg atgtcacgcg gctgtcggac gtggccggga atggcccgcc
     4621 atggaccatg gcgtgagcga cctcgacgcc ggtgccaaag ccgttgacca ggatgcggcc
     4681 ggccttcagc tccaggtcgg gcaacagatc ggcggccagg acgtggtcgg cttcgtccat
     4741 gtgcagggcg atggtcagct ggccttcgag ggccacgacc accttgtgaa gctcggccag
     4801 atcggcgcag cgcaccacga tcgaggcggc gccgaacacc tcttcggcca gatgcggatg
     4861 ggccaggaag tcggcgccgg aaacgccaaa cagcgctgcc tgcccctggt tctgtaaggc
     4921 ctcctgcccc acggcgacct gtttgacgac aggagtctgg gccagggccc gcaccccgtc
     4981 agcatagacg cgcgagatgg ccggggtcag catggtcgcg gccggaaccg gcgacagcgc
     5041 ctcttcggcg gcggtcagga acccgtccag ctccgggccg tccagcgcca ggatcaggcc
     5101 gggattggtg cagaactggc ccgcgcccag ggtcagggcg ccgacgaagt ccctggctat
     5161 agccgggccg cgcgcggcca gggcagcggg aaacaacaac acaggattga tgctgctcat
     5221 ctcggcataa aacgggatgg gctccggccg cccctgggcg atggcggcca gcgccagacc
     5281 gccgcgacgc gacccggtga agccggcagc cttgatgcgg ggatcggcga ccagcgcctg
     5341 gctgatctcg aagccgctgt catggatcag gctgaagacg cccggatgca ggccgcaggc
     5401 ggcgaccgcc gactggatgg cgcggccgat catttccgac gtgccgggat gggcggggtg
     5461 ggccttgacc accaccgggc agccggccgc cagggccgag gcggtgtcgc cgccggccac
     5521 cgagaaggcc agcggaaagt tggaggcccc aaacacggcc accgggccga gggggatgtt
     5581 gcgcaggcgc agatcggggc gcgccatcgg cttgcggtcc ggcatggcgg ggtcgatacg
     5641 ggcctccaga aagccgccgt cgcgcagcac gctggcgaac aggcgcaact ggccggtggt
     5701 gcggccgcgc tcaccttcca gccgggcgcg cggcaggccg ctttcggcca tcacacggac
     5761 gatcaggtca tcgcccagcg cttcgatatg ctcggcgatg gcctcaagga aggtcgcgcg
     5821 ggtctcatag ggcagagcgc ggtagggctg gaaggccgcc gccgcctggg cgcaggccgc
     5881 ctcgagattc tggagcgtgg cgccaccaaa ggccgggtcc agcacctcgc ccgtggcggg
     5941 gttgacgccg cgaatttcac cctgggcgcc gcgcgtcgcg ccgcccccga tcagcagttc
     6001 accggtcaga ttcacggaag cgttccctgt tcgaagtgat gcgtcgctcg tcgttgcgac
     6061 taggtagcgg taacataagc ctttggttgg gttaccgcca tgtgtgagaa tggatggcga
     6121 gcccttgagc gcgtaatagg gcctgacttc gtcaggatct tgaacatggc ccgcaatctt
     6181 tccggcaaag tcgtcgcctc cggcgccgat gtcaccgccc tggccgacaa ggcctggttc
     6241 gacgcccatc ccgagcggga cttcatgcta cgcgatcctg cgccgctgga gttcagggaa
     6301 ccgctgggcg acgccggcga gggtttcagc tggcgggttc tgatcgtgcg actcggcgac
     6361 ggctcgcgcc tgcgcctgcc gatctccctg gcctgggacc tgcacaatga tcacgccaaa
     6421 gagcagcatc tggccgtgat cttcgagcag gtcgcgccgg agcaggccag ggtcttgcgc
     6481 gccgcggccc tcgccggggc gccgcgcgtc tagcggcctt cagaacctat tcggcgtcat
     6541 ctggatcgca ctgcggcttt ccgatcctgc attgcgcacc ggcccacagt ccacgccacc
     6601 atcgcggcgc cggatccagt ttcaccattg ctaagcttgc attgcagtgc gcgccgataa
     6661 atctgacctg atctggagaa atattccgat ctgatgctca ttttcccgcg actgccgaat
     6721 acgcggaaat ttcttcgctt ctacaggcgt tgcgctctca agaatttccg cgtatggtgt
     6781 tcgtattaaa tgacgatttg gttcgattgc tctctaacgc ttcatctcag gattgagctt
     6841 cgagcccatc acgatccttt ccgaaaattt gatcacgtgg cttacattcg agccgcgcgt
     6901 cccttttcac ctgaaaggtg accatcatga cgaccggtac cgtgaagtgg ttcaactcca
     6961 ccaagggctt tggcttcatt caacctgacg acggcggcgc cgacgtgttc gtgcacatct
     7021 cggcggtcga gcgttcgacc ctgggctcgc tgaacgaagg ccagaagctg tcctacgaac
     7081 tcgagcgcga tcagcgcagc ggcaagatgt cggccggcca gcttcaagct gcctaagcca
     7141 cttcggagcg cgggccagtc ccgcgctcca gcctggcttt tctacctagc gacctcgaac
     7201 tgacacg
//
LOCUS       DAOMEO010000016         7187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1508_length_7187_cov_2.626753, whole genome shotgun sequence.
ACCESSION   DAOMEO010000016 DAOMEO010000000
VERSION     DAOMEO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_7187_cov_2.626753"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>538)
                     /locus_tag="PLH31_00745"
     CDS             complement(<1..>538)
                     /locus_tag="PLH31_00745"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR87810.1"
                     /translation="ERIFYGTSAEVRAFEVGGKSAPVPIFPSHDHLAATGSEGQFDGL
                     SALWTPGVGESLRDLYLGRAKAYGLMDSGKPFFTDKMPLSEIWTPVIRAAFPEARLIR
                     MVRHPLDTAVSMLSHNLTHGDYCGYRIEDIASHMQAVHALTGSYAATLGDTALAVRYE
                     DLVTDQETWTRRLLEHLDL"
     assembly_gap    539..548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<549..1560)
                     /locus_tag="PLH31_00750"
     CDS             complement(<549..1560)
                     /locus_tag="PLH31_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR87811.1"
                     /translation="MAADAQTHPLIVQARELLNQGQVEPALATARRRLAEVASDIDAL
                     EIVAMVCQQRGQVAEAIALLEKIINLEPRLDWPRDDLAILLAGRGRRTEAEAVTRAAL
                     TRNARNPQAHAMLGTLLTERRDLPGGEAAFRRALALVGDHPPTQVALAANLIEQGKLE
                     TAELLLARADQLAPGDGATLAHWSRLREAIGDLTASRALLDRAEAAGFDVTLRRAVQL
                     GREQRHAEALAVIEAVPGRKGGDALLESGRLKEKLGRPAEAFADFVEGKARLATRDGL
                     SYDGVAVSREFDTLARLVQTKGSPPGAARREAPQPVFVFGFPRSGTTMVEQILSSHTA
                     VAA"
     gene            complement(1565..2767)
                     /locus_tag="PLH31_00755"
     CDS             complement(1565..2767)
                     /locus_tag="PLH31_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQR87812.1"
                     /translation="MTDEISAEAWLQQAVAASNEGRHPEAQDGFVRALSIDPTLGEAM
                     LGLGLSYMATRDFKAAVAPLRQAAAAPEATAIWQACLAQGLYMTGDFQGCAEAFERAA
                     ALEPLATNARLTYARARTFASMIEGSVDEALSGYPALAGDEAEDIEVIAREAFAILGV
                     FGHAPAAAAVGAWRLARNPDDQIQAYLLQAVSGAAAVDRAPAAYVEAHFDSFADNFDR
                     QLVDLLNYRAPERMAELVAGHQDRFTDILDLGCGTGLSAPALARFGGRLTGVDLSRGM
                     LDRAKARGGYDALIQGEAIAFLSEHPAAFDLVFAADMVIYFGDLSDLFRSAKTALRPG
                     GVFALSTEVGAEGWTLLSSGRFSHADAYVAQAAGPDFHLLDQERIALRHEGTAPVEGT
                     LHVFQRRA"
     gene            complement(3007..3096)
                     /locus_tag="PLH31_00760"
     tRNA            complement(3007..3096)
                     /locus_tag="PLH31_00760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3060..3062),aa:Ser,seq:cga)
     gene            complement(3203..3622)
                     /locus_tag="PLH31_00765"
     CDS             complement(3203..3622)
                     /locus_tag="PLH31_00765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQR87813.1"
                     /translation="MAEDFSETLTQRQSGNLPDILGLEWLEARHGYVKGRFTVAKHHM
                     APNGFLHAASVIALADSASGYGCMVSLPEGGSGFTTIELKSNFLGTVREGGVLCEAKL
                     THGGRQTQVWDAVVTAEATGKTIALFRCTQMVLHPRA"
     gene            complement(3740..4810)
                     /gene="mnmH"
                     /locus_tag="PLH31_00770"
     CDS             complement(3740..4810)
                     /gene="mnmH"
                     /locus_tag="PLH31_00770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HQR87814.1"
                     /translation="MKPLQVLKSADPAALAAFDQIIDVRSPGEFAQDHLPGAINLPVL
                     DDAQRAEVGTIYVQDSRLRANRIGAAHVARNIARHLDTAFADRTEDFKPLIYCWRGGQ
                     RSNAMATILQRVGWQTAVLDGGYRTYRRWVQARLYDTPLNLDLILLDGDTGSAKTAIL
                     GALAERGVQIVDLEGLACHRGSLFGALTGQEQPDQKRFESQLILAFDALDPSRPVVVE
                     AESSKIGNRVLPPMLWTAMRKAPRVEVTAPVSERARYLVETYGDITARREALLAILER
                     LPIHPSRRRLAAWCEMVESGAYADLAEALMSAHYDPAYARERRKADQPLLASIALERL
                     DPASLDLAATRIIDSIGISART"
     gene            4940..6007
                     /gene="selD"
                     /locus_tag="PLH31_00775"
     CDS             4940..6007
                     /gene="selD"
                     /locus_tag="PLH31_00775"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQR87815.1"
                     /translation="MSESPPAPVRLTSLAHGGGCGCKLSPAVLRDILAQSPAAGIFPN
                     LMVGTETSDDAAVWRLNDQQALVATTDFFMPVVDDPHDFGRIAATNALSDVYAMGATP
                     IFALAIVGMPIDRLPTETIGAILAGGAAVCAAAGVPIAGGHSIDSVEPIYGLVALGLV
                     HPDRVLSNRGAQAGDVLILTKALGVGVLSAAFKQQRLSAEGYGALIATTTQLNTVGAG
                     LGEVAGVHAVTDVTGFGLLGHLLEMCRGAGLAAELDADAPALLPGAAGLAQAGVRTGA
                     AVRNWASYGAAVQMPGGTPDWRRDLLADPQTSGGLLIAVAPEAADAVLRKVTEAGFIA
                     ARVVGRMTPGAPAVKVRAAFD"
     gene            complement(6004..>7187)
                     /locus_tag="PLH31_00780"
     CDS             complement(6004..>7187)
                     /locus_tag="PLH31_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQR87816.1"
                     /translation="RAYRSSWLSYLVGLAIVCSGLGSIATQSKVVAENLNTLIGLDFG
                     FTIAGASAEVMVLAIGFLLLVAGIVYRGITESLWANAICTLVESLGLLLVIVVGVRFC
                     GQADLFEVPVSAANPAGALTVGLLLQGSILTFFSFLGFEDMLNVAEEVKQPERTIPLA
                     LIGAMVLASVIYMAVSITAVSVVPWRELAEAPGPLKLVIERAAPWFPAVGFTLITIAA
                     VANTALVNYVMGSRLLYGMARQGLLPSVLGRVHGARQTPHVAIAVLLVIVIGLQFAGG
                     IEQLASATVLLLLLVFAVVNGALIVLTHRDGKIEGCFNAPIAIPAAGVLICLVMIVGR
                     VQQDDWRGPAIAAGLIAMILALYVLTRKSRRAGGVRRKTRHALATDRPPPSRNRPDRP
                     V"
BASE COUNT         1190 a         2358 c         2461 g         1168 t
ORIGIN      
        1 caggtccaga tgctccagca ggcgccgtgt ccaggtttcc tggtcggtca ccagatcctc
       61 gtagcgcaca gccagcgccg tatcgcccag cgtcgctgca tagctcccgg tcagcgcgtg
      121 gacggcctgc atatggctgg cgatatcctc gatccggtat ccgcagtaat cgccgtgggt
      181 cagattgtgc gacagcatcg acaccgccgt atccaacgga tgacgcacca tgcggatcag
      241 cctcgcttca gggaaggcag cgcggatgac cggcgtccag atctcactca gtggcatctt
      301 gtcggtgaag aacggcttgc cgctgtccat cagtccgtag gccttggcgc ggcccagata
      361 gagatcccgc aagctctcgc cgacgccggg cgtccatagc gccgacaggc cgtcgaactg
      421 gccctccgaa ccggtggcgg ccaggtgatc gtggctgggg aaaatgggga cgggagcgga
      481 ttttccgcca acctcaaagg cacggacttc agccgacgta ccgtagaaaa tccgctccnn
      541 nnnnnnnncc gccgcgacgg cggtatgact ggacaggatc tgttcgacca tggtggtgcc
      601 ggagcggggg aagccaaaca cgaacactgg ctggggggcc tcacgccgcg ccgcgccggg
      661 cggcgagccc ttggtctgga ccagtcgcgc cagggtgtcg aactctcggc tgaccgccac
      721 gccgtcatag gagagcccgt cgcgggtggc gaggcgggcc ttgccctcca cgaagtcggc
      781 gaaggcctca gcgggacggc cgagcttctc cttcagccga ccgctttcca gcagggcgtc
      841 gccgcccttg cgaccgggaa ccgcctcgat cacggccagg gcctcggcgt ggcgctgttc
      901 gcgacccagc tggacggcgc ggcgcagggt gacgtcgaaa cccgccgcct cggcccgatc
      961 cagaagagcc cgactggcgg tcaggtcgcc gatcgcctcc cgcagacgcg accagtgggc
     1021 gagggtcgcg ccatcgcccg gggccagctg gtcggcgcga gccaacagga gttccgccgt
     1081 ctccagcttg ccctgttcga tcagattggc ggcgagagcg acctgggttg gcggatggtc
     1141 gccgaccagg gcgagggcgc gccgaaacgc cgcctcgccg cccggcaggt cgcgccgctc
     1201 cgtcagcagg gttcccagca tggcgtgagc ctggggattg cgggcattcc gcgtcagggc
     1261 agcccgcgtc accgcttcgg cttcggtcct gcgaccccgt ccggcgagca ggatcgccag
     1321 gtcgtcgcgc ggccagtcca ggcgcggttc cagatttatg atcttctcca gcaaggcgat
     1381 ggcctcggcc acctggccgc gctgctggca gaccatggct acgatctcca gggcgtcgat
     1441 gtcgctggcc acctcggcca gtcgccgccg cgccgtcgcc agggccggct cgacctgacc
     1501 ctggttgagc agttcgcgcg cctggacaat cagggggtgg gtctgtgcgt cggccgccat
     1561 caggtcaggc gcgccgttgg aaaacgtgga gcgttccctc gaccggcgct gtcccttcgt
     1621 ggcgcagggc gatgcgctcc tgatccagca gatggaagtc ggggccagcc gcctgcgcca
     1681 cataggcgtc ggcgtgggag aaccggcccg agctgagcag ggtccagcct tccgccccga
     1741 cctcggtgct cagggcgaag acgccgcccg gcctcagggc tgttttggcg ctccggaaaa
     1801 ggtcggaaag gtcgccgaaa tagatcacca tgtcggccgc gaagaccagg tcaaaggcgg
     1861 cgggatgctc gctgaggaac gcgatggcct caccctggat caaggcgtca tagccgccgc
     1921 gcgcctttgc gcgatccagc atcccacgcg acagatcaac gcccgtcagc ctgccgccaa
     1981 accgcgccag ggccggcgcc gatagcccgg tgccgcagcc cagatccaga atgtcggtga
     2041 accgatcctg gtgacctgcc actagctccg ccatccgctc gggcgcgcgg taattgagca
     2101 ggtcgaccag ctggcggtcg aaattgtcgg cgaaactgtc gaaatgggcc tcgacatacg
     2161 ccgccggcgc tcgatccacc gctgcggcgc cgctgacggc ctgcaacaga taggcttgga
     2221 tctggtcatc cggattgcgc gccagccgcc aggcgcccac cgcggccgct gcgggagcgt
     2281 gtccaaacac gccgaggatc gcaaaggctt cgcgggcgat gacctcgatg tcctcggcct
     2341 cgtcgccggc cagggcgggg taaccgctca gcgcctcgtc aacggagcct tcgatcatgc
     2401 tcgcgaaggt gcgcgccctg gcgtaggtca ggcgcgcgtt ggtcgccagg ggctcaagag
     2461 ccgcggcgcg ttcgaacgcc tccgcgcagc cctggaagtc gcctgtcatg tagaggccct
     2521 gggccaggca ggcctgccag atcgccgtgg cctccggcgc cgccgctgcc tggcgcagag
     2581 gggcgaccgc cgccttgaaa tcccgtgtcg ccatatagga aaggccaagg ccaagcatcg
     2641 cctcgcccaa ggtcgggtcg atcgacaggg ctcggacaaa tccatcctgc gcctcgggat
     2701 gtcgcccttc gttactcgct gcgacggcct gctgaagcca ggcctcggcg ctgatctcgt
     2761 cggtcacgga aacctcctga gggcggtgtt tgccgttggc cctgatcgac gtcatctaac
     2821 gtgtccggtt ggagacgggc aatccagacc atcaaagtcc gggactgaac atctgggctg
     2881 atgatcctga gccctttcgc cccacctctg cgaagctgcc atatcggaaa gcgctttcag
     2941 cgcaccattg atcggctctt acagaaaggc tgctcgggcg ccgacgggct tcactgtatg
     3001 gggtggtggc ggagagggtg ggattcgaac ccacggtgcc cttccgggca cgccgcattt
     3061 cgagtgcggt acattcgacc actctgccac ctctccaggc gccgttcgtg agcccttttg
     3121 gggagggccc gaggtcgagg gcgcgatacc tagcggtgtc gtcgcggtaa cgcaagtccc
     3181 tgtcggaggc tttctgccgc cgtcaggcgc gggggtgcaa aaccatctgg gtgcagcgga
     3241 aaagtgcgat ggtcttgccg gtcgcctcgg cggtgacgac ggcgtcccag acctgggtct
     3301 ggcggccgcc atgggtcagt ttcgcttcgc acaggacacc gccttcgcgc acggtgccca
     3361 ggaagttcga cttcagttcg atggtcgtga agccgctgcc gccctcgggc agggacacca
     3421 tgcagccata gccgctggcg ctgtcggcca gggcgatgac gctggcggcg tgcaggaagc
     3481 cgttgggggc catgtgatgc ttggcgacgg tgaagcggcc cttcacatag ccgtggcggg
     3541 cttcaagcca ttccaggccc aggatatcgg gcagattgcc ggactggcgc tgggtcaggg
     3601 tttcgctgaa gtcttcggcc atcatgccct cctcgtgttt cgtgcgccaa tcctatggcg
     3661 gcagacaggc tgggggcgca agtgcgtttc taaaaagaac ggatgtgtca gcgagatagc
     3721 cgccgcgccg gcgggaccgt catgttcggg ccgaaatccc tatgctgtcg atgatcctgg
     3781 tcgcggccag atcaagcgag gccgggtcca ggcgttccag tgcgatgctc gccagcaggg
     3841 gctggtcggc ctttcgacgc tcgcgtgcat aggctgggtc atagtgggcg gacatcaggg
     3901 cttcggccag atccgcgtag gcgccgctct ccaccatctc gcaccaggcc gccagtcgcc
     3961 gtcggctggg gtggatcggc aggcgctcca ggatcgccag caaggcctcg cgccgcgcgg
     4021 tgatatcgcc ataggtctcg accagatagc gggcgcgttc cgatactggc gcggtgactt
     4081 caacgcgcgg ggccttgcgc atcgccgtcc acagcatcgg cggcaacacg cggttgccga
     4141 tcttgctcga ctccgcctcc accacgaccg ggcgagaggg atccagcgca tcgaacgcga
     4201 ggatgagctg actctcgaag cgcttctgat cgggctgctc ctggcctgtc aaagcgccaa
     4261 acagcgagcc gcggtgacag gccagaccct ccagatccac gatctggacg ccacgctcag
     4321 ccagggcgcc gagaatcgcc gtcttggccg agccggtgtc gccatccagc aggatcaggt
     4381 ccaggttcaa gggcgtgtca tagagcctgg cctggaccca gcgacgatag gtcctgtagc
     4441 cgccatccag tacggcggtt tgccagccca cccgctgcag gatcgtggcc atggcgtttg
     4501 acctctgccc gccgcgccag caatagatca ggggcttgaa gtcttccgtg cggtcggcga
     4561 aggcggtgtc cagatggcgg gcgatgttgc gggccacatg ggccgcgcca atccggttcg
     4621 cgcgcagccg cgagtcctga acatagatcg tcccgacctc ggcgcgctga gcgtcgtcaa
     4681 gcaccggcag attgatggcg cccggcaggt gatcctgggc gaattcgccg gggctgcgca
     4741 catcgatgat ctggtcgaac gccgccaggg cggcggggtc tgcggatttg agaacttgaa
     4801 gcggcttcat gtccgccttg ggtttcgccg tgtcgtgaac atcattccgc aggatctcgt
     4861 cagcgccgca tgtctttccg cgactatgcg cgacacacgg ggaaatcgta aggtcccggc
     4921 gtagttccag gagcgcttcg tgtcagaatc cccgccggcc cccgttcgac tgacctcgct
     4981 ggcccatggc ggcggatgcg gctgcaagct gtcgccggcg gtgctgcgcg acatcctcgc
     5041 ccagtcgccg gcggcgggga tctttcccaa cctgatggtc ggaaccgaga cttccgacga
     5101 cgccgccgtc tggcgactga acgaccagca ggccctcgtc gccaccacgg actttttcat
     5161 gccggtggtt gacgatcccc atgactttgg tcgcatcgcg gcgaccaacg ccctgtcgga
     5221 tgtctatgcc atgggcgcga cgcccatttt cgctctggcg atcgttggca tgccgatcga
     5281 caggctgccg acagagacca tcggtgcgat cctggccggc ggcgccgcgg tctgcgcggc
     5341 ggcgggcgtg cccatcgcag gtgggcactc gatcgacagc gtcgagccaa tctatgggct
     5401 ggtggcgctt ggactggttc atcccgatcg ggttctgtcc aaccgcggcg cccaggccgg
     5461 cgacgtgctg attctgacca aggcgctggg ggttggcgtc ctgagcgcag cgttcaagca
     5521 gcagcggctt tcggctgagg gctatggcgc cttgatcgcg accaccaccc agctcaacac
     5581 ggtcggggcg gggctgggcg aagtcgccgg ggttcacgcg gtcaccgacg tcaccggctt
     5641 tggcctgctg ggccacctgc tggaaatgtg ccggggcgcg gggctggccg ccgagcttga
     5701 tgctgacgcg ccggccctgc tgcccggcgc cgccggcctc gcccaggccg gggtgcgcac
     5761 cggcgccgcg gtgcgcaact gggccagcta cggggcggcg gttcagatgc cgggggggac
     5821 gcccgactgg cggcgcgacc ttctggccga tccgcagacc agcggcggtc tgctgatcgc
     5881 cgtcgcgccc gaggccgccg acgctgttct gaggaaggtc accgaagcgg ggttcatcgc
     5941 cgccagggtg gtcgggcgga tgacccccgg cgcgccagcg gtgaaggttc gggcagcctt
     6001 cgactaaacc ggacggtcgg gacggttccg ggaaggagga ggtcgatccg tcgcgagcgc
     6061 gtggcgggtc ttccgtcgca ccccgccggc gcggcggctt ttgcgggtca ggacatagag
     6121 ggcgaggatc atcgcgataa ggccggccgc gattgcgggg cctcgccagt catcctgctg
     6181 tacgcgaccg acgatcatca caaggcagat caggacgcct gcggccggta tggcgatcgg
     6241 cgcattgaag cagccctcga tcttgccgtc ccgatgcgtc aggacgatca gggcgccgtt
     6301 aaccaccgcg aagaccagaa gcagcagcaa caccgtcgcg ctggccagtt gctcgatccc
     6361 gcccgcgaat tgcaggccga tgacgatcac cagcagcacg gcgatcgcga catgcggtgt
     6421 ttgacgcgcg ccgtggacgc ggcccagaac ggacggaagc agcccctggc gcgccatgcc
     6481 gtacagcagg cgagagccca taacgtagtt gaccagggcg gtgttggcga ccgcggcgat
     6541 ggtgatcagg gtgaagccga cggcggggaa ccacggcgcc gccctctcga tcaccagttt
     6601 gagggggcca ggcgcctcgg cgagttcccg ccagggcacg actgaaacgg ctgttatcga
     6661 gaccgccata tagatcaccg aggcgagaac catggcgccg atcagcgcga gcgggatcgt
     6721 tcgctccggc tgtttgacct cctcggcgac gttgagcatg tcttcgaagc cgaggaatga
     6781 gaagaaggtc aggatggagc cttgcagcag caatcccacg gtcagcgcac ctgcgggatt
     6841 ggcggcgctg acggggacct cgaaaagatc cgcctggccg cagaagcgca cgccgacgac
     6901 gatgaccagc agcaggccca gagactcgac cagcgtgcag atggcgttgg cccagagcga
     6961 ctctgtgatg ccgcgataga caatgccggc caccagcagc agaaatccga tcgccagaac
     7021 catgacctcg gcggaggcgc cggctatggt gaagccgaaa tccaggccga tgagcgtatt
     7081 gaggttctcg gcgaccacct tggattgggt ggcgatggac cccagtccgg aacagacgat
     7141 ggccaggcca acaagatagc tgagccagga gctgcgataa gcccgct
//
LOCUS       DAOMEO010000017         7161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1512_length_7161_cov_2.450042, whole genome shotgun sequence.
ACCESSION   DAOMEO010000017 DAOMEO010000000
VERSION     DAOMEO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_7161_cov_2.450042"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PLH31_00785"
     CDS             <1..1432
                     /locus_tag="PLH31_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR87817.1"
                     /translation="TIETSCARVFLIGDAAFKVKRPVDFGFLDYSTLELRRWALEREL
                     TFNRAAAPDIYRTVRRLTRDAQGGLEVDGEGEVVEYALEMRRFDEQGVLATQPWAIDD
                     ALEDSLGRTVARFHAGATLRPQGGGVHALGYTIRSNANLLRGLAPRLGSLAVERLIAE
                     TDLALDRLGPLLDARAAEGFARHCHGDLHLGNILIENGQPILFDCIEFNDTLSDIDIQ
                     YDLAFLLMDLDFRRRRDAAGRVLNAYLDEAARAFGAGLWSGLAALPLMLSVRAAVRTH
                     VWAYTGDDEAARAYLATALEHLAPRPVSLVATGGLSGSGKSTFARVCAPGLGSAPGAV
                     VLRTDEIRKRLWGVPSLQRLPREAYTPEMSVRVYDQLFDDAALALKAGRSVVLDAVFM
                     KPEERARAEALAAAADVAFQGVWLEAPPEVLRARVAARVNDASDADVAVLETQLTRET
                     GEIAWRRVDTVSAFEDEARTLAETLG"
     gene            complement(1448..3166)
                     /locus_tag="PLH31_00790"
     CDS             complement(1448..3166)
                     /locus_tag="PLH31_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR87818.1"
                     /translation="MDTHRLLPSRLISALLASVVMAPALGLPTAMAWLACLLAGEGLA
                     RLATRRFRPGVTGSPVLRAAFVLAALPINITWSGLAAMLWLSDIADLKLAALAVWVGQ
                     IVYTQNFRHQPWTLIAVSGVAPLTSLIVFPFFFYDAQGIGPQTARWGLLIVTLSTINT
                     MIANRAAARRMDELTNGLREERERALEATRAKSTFIAVTSHELRTPMNGLLGMAHALS
                     RSGLNETQREQVRLMIRSGDNLMQLLNDVLDLSRIEAGKVELTPAPFRLEETVRDVVD
                     AWRDVAMVKNLDIALRFDADAPAWILGDALRIRQVLTNLISNALKFTTEGGVEVHVHC
                     PVPEIEGRRAIVFAVRDTGPGVPPEATDRVFESFTQADDTISRRHGGVGLGLTISRAL
                     ARQMGGDLTLEPSKTGACFKFSIRAPIAEPQLAANPAEDEREGAMASIRVLMAEDNEI
                     NQLVVQTMLEATGLSLTVVDHGQAALDALADGHFDCVLMDINMPVMDGITALEAIRSG
                     AAGDPALPVIALTASTMAGDRERFLEMGFNDHLGKPVKPAALINAIARAVDPDAPRAE
                     ALHDAA"
     gene            3459..6251
                     /locus_tag="PLH31_00795"
     CDS             3459..6251
                     /locus_tag="PLH31_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HQR87819.1"
                     /translation="MQGLMQHGALTVDKIIDHAAQWHGSREVVTRSVEGPIVRTTYGE
                     IHNRAKRVSNALLSLGIKPGDRVATLAWNTGRHMEAWYGIMGIGAVCHTLNPRLFPEQ
                     IAWIADHAGDRVLFTDLTFLPIVAAILSKVPSIEHVVVFTDGAHMPAFAPAAEAPHFK
                     GVHCFETLIDSHGADCAWGGFDEGTAAGLCYTSGTTGDPKGVMYSHRSNFLHTLITLQ
                     PDVMGLSQRDVILPVVPMFHANAWGVAFSAPGTGAKMVMPGAKMDGASIYELLETEGV
                     TFSAAVPTVWQMLLQHLRENDLKLPVLKKVVIGGAACPEHIIRAFQEDYDVEVVHAWG
                     MTETSPVGTLSVMTEELLKLPYDQQMPYRLKQGRPPLGVELKLTDDAGQRLPHDGRSF
                     GNLKIKGPIIAGEYFRGAGGKILDDEGFFDTGDVATIDEHGFMQITDRAKDVVKSGGE
                     WISTIDIENIAMGHPKAALAAVIGVAHPKWDERPILLVKLKEGETATKEEFLAFLDGK
                     IAKWWMPDDVLFVDDIPLGATGKIDKKLIRQRMKDYVLPTAAAAPVLAAADGGQAARI
                     YAPDPVETPPDVEAIASGELEAGGIDVESFPAEAELTPVVAAEAEALPPPEDLDDQPL
                     RLREPDAPPPVATPVATPVASAADDLPFAMPLTPRDQRKAKAAVAKAGQTPAWAPLYL
                     DLALVIALIPGLLVGAGALGVKLGLLDLSLGYTLMAQDWAPKLAYLSVATGVLGLIIA
                     LMVGFGKLWKRVLLILLITVATIAAMLGVNAAGGRAPPVHDVSTDWKTPLGFSDAALA
                     ARGGSAQAVEDDPSLPVGSVVFAGRRVADVNAETCPAARPLVLERSPADAYEAAKAAV
                     LAAGLTLITDDPMDGRLEATGRSYWYGLVDDLVVRVRPDAAGARVDMRSIGRDPGPDQ
                     GRNCRRIEGLLTAVKG"
     gene            complement(6295..>6560)
                     /locus_tag="PLH31_00800"
     CDS             complement(6295..>6560)
                     /locus_tag="PLH31_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR87820.1"
                     /translation="GAPVRDVEPFIAAYIAHRRAREVQILDALAAGHTQIRAMVPSLY
                     AAVDPRLHPAAAMSVLAHMQQLAREGRVLCDGAPDLDATYRLP"
     assembly_gap    6561..6570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6571..>7161)
                     /locus_tag="PLH31_00805"
     CDS             complement(<6571..>7161)
                     /locus_tag="PLH31_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR87821.1"
                     /translation="GRCDQVSPLIRRVIARNPGPFTYTGTGVYIVGSGEVAVIDPGPE
                     IAEHLEALLAAVKGERVTHILVTHHHLDHSPLARPLAELTGATIYGRAAPADHGEAHA
                     GLEEGADGRFTPDVELADGQVLSGPGWTLEAITTPGHTSNHVCFALKEENALFCGDHI
                     MGWSTTVITPPDGDMGDYFASLEKVKARSFDTLWPTH"
BASE COUNT         1118 a         2384 c         2505 g         1144 t
ORIGIN      
        1 caccatcgag acctcatgcg cccgggtgtt cctgatcggc gacgccgcct tcaaggtgaa
       61 gcgaccggtt gatttcggct ttctggacta ttccaccctg gagttgcggc gatgggcgct
      121 ggagcgggag ctgaccttca accgcgccgc cgcgccggac atctatcgca ccgttcggcg
      181 actgacccgt gacgcccagg gcggccttga ggtcgatggc gagggcgagg tcgttgaata
      241 tgctctggag atgcgccgct ttgacgagca gggcgttctg gccacacagc cctgggcgat
      301 cgacgatgcg ctggaagact cgctgggtcg caccgtggcg cgtttccacg ccggggccac
      361 cctgcggccg cagggcgggg gcgtccacgc cctgggctac acaatccgct caaacgccaa
      421 tctgctgcga ggcctggcgc cgcgtctggg cagcctggcg gtcgagcgcc tgatcgccga
      481 gacggatctg gcgctcgacc gcctcggccc cctgctggac gcccgcgcgg cggagggctt
      541 tgcccgccac tgtcatggcg acctgcatct gggcaatatc ctgatcgaga acggtcagcc
      601 gatcctgttt gactgcatcg agttcaacga caccctgtcg gacatcgaca tccagtacga
      661 tctggcgttc ctgctgatgg acctggattt ccgccgtcgt cgcgacgcgg ctggccgggt
      721 tttgaacgcc tatctcgacg aggctgcccg ggcgttcggc gccggcctgt ggtcagggct
      781 ggccgccctg ccgctgatgc tgtcggtgcg cgcggccgtt cgcacccacg tctgggccta
      841 tacgggcgac gacgaggcgg cgcgggccta tctggccacg gctctggagc atctggcccc
      901 gcgccccgtc agcctggtgg cgactggcgg cctgtcgggc tcgggcaaat cgaccttcgc
      961 ccgggtctgc gcgccgggcc tgggctcggc gccgggcgcg gtggttctgc gcaccgacga
     1021 gatccgcaag cgcctgtggg gcgtgccctc gctgcagcgc ctgccgcgcg aggcctatac
     1081 gccggagatg agcgtccggg tctatgacca gctgttcgat gatgcagccc tggccctgaa
     1141 ggcgggtcgt tcggtggtgc tggacgcggt gttcatgaag ccggaggagc gcgcgcgcgc
     1201 cgaggccctg gccgccgcgg ccgatgtggc ctttcagggg gtctggctgg aagccccgcc
     1261 cgaggtgctg cgcgcccgcg tcgccgcccg ggtcaatgac gcctcggacg ccgacgtcgc
     1321 cgtgctggaa acccagctga cccgcgagac cggcgagatc gcctggcgca gggtcgacac
     1381 ggtcagcgcc tttgaggacg aggcgcggac cctggcggag actttgggct aggggccagg
     1441 aaatactcta ggcggcgtcg tgcagcgcct cggcgcgcgg cgcgtcgggg tcgacggcgc
     1501 gggcgatggc gttgatcagg gcggccggct tgaccggttt gcccagatga tcgttgaagc
     1561 ccatctccag aaagcgctcg cggtcgcccg ccatggtcga ggccgtcagg gcgatgaccg
     1621 gcagggcggg atcaccggcc gcgcccgaac ggatcgcctc cagcgccgtg atgccgtcca
     1681 tcaccggcat gttgatgtcc atcagcacgc agtcgaagtg gccgtccgcc aaggcgtcca
     1741 gcgccgcctg tccgtgatcg accacggtca gggacagccc tgtggcttcc agcatggtct
     1801 ggaccaccag ctggttgatt tcattgtcct cggccatcag tacgcggatc gaggccatcg
     1861 cgccttcgcg ttcgtcctcg gccggattcg cagcgagctg gggctcggcg atcggcgcgc
     1921 ggatgctgaa cttgaagcag gcgccggtct tggacggctc cagcgtcagg tctccgccca
     1981 tctggcgggc cagggcgcgg gagatcgtca gcccaaggcc gacgccgcca tgccggcgcg
     2041 agatcgtgtc atcggcctgg gtgaagctct caaagacccg gtctgtcgct tcgggcggta
     2101 cgcccgggcc ggtgtcgcgc acggcaaaga cgatggcgcg ccgtccctcg atctcaggga
     2161 ccgggcaatg aacatgaacc tcgacgccgc cctcggtcgt aaacttcagg gcgttggaga
     2221 tcaggttggt caacacctgc ctgatgcgca gggcgtcacc caggatccag gcgggggcgt
     2281 cggcgtcgaa ccgcagggcg atgtccaggt tcttgaccat ggcgacgtcg cgccaggcgt
     2341 cgacaacatc gcgcacggtc tcttccagcc tgaacggcgc aggcgtcagc tcgaccttgc
     2401 ccgcctcgat acgcgacagg tccagcacgt cattgagcag ttgcatcagg ttgtcgccgg
     2461 accggatcat cagccggacc tgctcgcgct gggtctcgtt gaggcccgag cgcgacagcg
     2521 cgtgggccat gcccagcagg ccgttcatcg gcgtacgcag ctcatggctg gtgacggcga
     2581 tgaaggtcga tttggcgcgg gtggcttcca gggcccgctc gcgttcttcg cgcaggccgt
     2641 tggtcagctc gtccatgcgc cgggcggcgg cgcggttggc gatcatcgta ttgatcgtgc
     2701 tcagcgtcac gatcagcagg ccccagcgcg ccgtctgcgg accgatgccc tgcgcgtcat
     2761 agaagaagaa cggaaacacg atcaggctgg tcaggggcgc gacgccgctg accgcgatca
     2821 gggtccacgg ctgatggcgg aaattctggg tgtagacgat ctgaccgacc cagaccgcga
     2881 gggccgccag ctttaagtcg gcgatgtcgg acagccacag catggcggcc agtccggacc
     2941 aggtgatgtt gatcggcagg gcggccagaa caaaggccgc gcgcaggacg ggcgagccgg
     3001 tgacgccggg cctgaacctg cgggtcgcca aacgcgccag gccttcgccg gccagaaggc
     3061 aggccagcca ggccatcgcc gtgggcagcc ccagcgcggg cgccatgacg acggacgcca
     3121 gaagggccga gatgagacgc gacggcagca ggcgatgggt atccatcgcc acctcgtcca
     3181 gccgttcgtc ggcccaaggg gattgcagca agcgcaagga attgtcctga tggggccagg
     3241 gcggccgccg agcgccaagg atactctttt aggggaaacg agaccttgag gcggcctgcg
     3301 acggatcagc gcgggcgcga gccgggcgcc ccgacgcaac cttacgccgg tcggcgaacg
     3361 ccaagatgat cgcccggtgc gcgcggcgcg ttgcgcggac aagctccgcc cgatagcttc
     3421 tcccgcataa caagaagcct ttcgggagag ccagttcgat gcaggggttg atgcagcacg
     3481 gggcgttgac ggtcgacaag atcatcgacc acgccgctca gtggcacggt agccgggaag
     3541 tcgtcacgcg gtcggtcgag gggccgatcg tgcgcaccac ctacggagag atccataatc
     3601 gcgccaagcg ggtttccaac gccctgctgt ctctggggat caagccgggc gaccgcgtcg
     3661 cgaccctggc ctggaatacc ggccgccaca tggaagcctg gtacggcatc atgggcatcg
     3721 gggcagtgtg ccacaccctc aatccgcgcc tgttccccga gcagatcgcc tggatcgccg
     3781 accatgccgg cgaccgcgtg ctgttcaccg acctgacctt cctgccgatt gttgcggcca
     3841 tcctgtccaa ggttcccagc atcgagcacg tggtcgtctt caccgacggc gcgcacatgc
     3901 cagccttcgc gccagccgcc gaggccccgc acttcaaggg cgtccactgc ttcgagaccc
     3961 tgatcgacag ccacggcgcc gactgcgcct ggggcgggtt tgacgagggc acggcggcgg
     4021 gactttgcta tacctcgggc acgacgggcg accccaaggg ggtcatgtat tcgcaccgct
     4081 ccaacttcct gcacaccctg attaccctgc agcccgacgt gatgggcctg tcgcagcgcg
     4141 acgtgatcct gccggtggtg ccgatgttcc acgccaacgc ctggggcgtg gcgttctcgg
     4201 ctcccggcac cggggccaag atggtgatgc cgggcgccaa gatggacggc gcctcgatct
     4261 atgagctgct ggaaaccgag ggcgtcacct tctcggccgc cgtgcccacc gtgtggcaga
     4321 tgctgctgca gcacctgcgc gaaaacgacc tgaagctgcc ggtcctgaaa aaggtggtga
     4381 tcggcggggc ggcctgtccc gagcacatca tccgcgcttt ccaggaagac tatgacgtcg
     4441 aggtcgttca cgcctggggc atgaccgaga cctcgccggt cggcaccctg tcggtgatga
     4501 ccgaggaact gctcaagttg ccctacgacc agcagatgcc ctatcgcctc aagcaggggc
     4561 ggccgccgct gggcgtcgag ctgaagctga ccgacgacgc cggccagcgc ctgccgcatg
     4621 acggcaggag ctttggcaat ctgaagatca agggcccgat catcgccggc gaatatttcc
     4681 ggggcgccgg cggcaagatc ctcgatgacg agggcttctt cgatacgggc gatgtggcca
     4741 cgatcgacga gcatggcttc atgcagatca ccgaccgcgc caaggacgtc gtcaagtccg
     4801 gcggtgagtg gatcagcacc atcgacatcg agaacatcgc catgggccat cccaaggcgg
     4861 cgctggcggc ggtgatcggc gtggctcacc ccaagtggga cgagcggccg atcctgctgg
     4921 tcaagctgaa ggagggcgag accgccacca aggaagagtt cctggccttc ctcgacggca
     4981 agatcgccaa gtggtggatg cccgacgacg tgctgttcgt cgacgacatc ccgctgggcg
     5041 ccaccggcaa gatcgacaag aagctgatcc gccagcggat gaaggactat gtcctgccga
     5101 ccgccgccgc cgcgcccgtt ctggccgccg ccgatggcgg gcaggccgcc cgcatctatg
     5161 ccccagatcc cgtggagacg ccgcccgatg tggaggcgat cgcctcgggg gagcttgagg
     5221 ccggggggat cgacgtcgag tccttcccgg ccgaagccga actgacgccc gtggtcgccg
     5281 ccgaggccga ggccctgccg ccgccggagg atctggacga ccagcccctg cgcctgcgcg
     5341 agcccgatgc gccgccgccc gtcgccacac cggtcgccac gcccgtcgcg tctgccgccg
     5401 acgacctgcc cttcgccatg cctttgacgc cacgcgatca gcgcaaggcc aaggcggccg
     5461 tcgccaaggc gggccagacc ccggcctggg cgccgctcta tctggacctg gccctggtca
     5521 tcgccctgat ccccggcctg ctggtgggcg cgggcgcgct gggcgtgaag ctgggcctgc
     5581 tggatctctc cctcggctac actctgatgg cccaggactg ggcccccaag ctcgcctatc
     5641 tcagtgtggc cacaggcgtg ctgggtctga tcatcgcctt gatggtcggg ttcgggaaac
     5701 tgtggaagcg ggtgctgctg atcctgctga tcaccgtggc aaccatcgcc gcgatgttgg
     5761 gggtcaacgc cgcgggcggt cgggcgccgc cggtgcacga cgtctctacc gactggaaga
     5821 cgccgctggg cttttccgac gccgccctcg cggcgcgtgg cggatcggcc caggccgtcg
     5881 aggacgatcc cagcctgccg gtcggctcgg tggtcttcgc cggtcgccgg gttgctgacg
     5941 tcaatgccga gacctgcccg gcggcgcgac cgctggtgct ggagcgcagc cccgcggacg
     6001 cctatgaggc ggccaaggcg gcggtgctgg ccgccggcct gaccctgatc accgacgatc
     6061 ccatggatgg ccggctggag gctacgggcc gcagctactg gtatgggctg gtggacgatc
     6121 tggtggtcag ggtgcgtcct gacgccgccg gcgcgcgggt cgacatgcgc tcgatcggcc
     6181 gcgatcctgg cccggatcag gggcgcaact gccgccggat cgaaggcctg ctgacggccg
     6241 tcaagggcta gagcattttt cgatccaact ggatcgatga aatgctctag cggctcaagg
     6301 cagcctgtac gtcgcgtcca ggtcgggagc gccgtcgcac agcactcggc cctcgcgcgc
     6361 caactgttgc atatgggcca ggaccgacat ggcggcggcc ggatgcaggc gcggatcgac
     6421 ggcggcgtag agcgacggca ccatcgccct gatctgcgta tggccggcgg cgagggcgtc
     6481 caggatctgg acctcgcgcg cgcgccgatg ggcgatatag gcggcgatga acggttcgac
     6541 gtcgcgcacg ggcgcgccat nnnnnnnnnn atgggtgggc cacagggtgt cgaagctccg
     6601 cgccttcacc ttctccaggc ttgcgaaata gtcgcccatg tcgccgtcgg gtggggtgat
     6661 gaccgtggtc gaccagccca tgatgtgatc gccgcagaag agcgcgttct cttccttcag
     6721 ggcaaaacag acatggttgg aggtgtggcc gggggtcgtg atcgcctcca gcgtccagcc
     6781 gggtcccgac agcacctggc cgtcggccag ttcgacatcg ggcgtgaagc ggccgtcggc
     6841 gccttcctca agtccagcat gcgcttcgcc gtgatccgcc ggggccgcgc ggccatagat
     6901 tgtggcgccg gtcagctcgg ccagggggcg ggcaaggggc gagtggtcca gatggtggtg
     6961 ggtgaccagg atatgggtca cgcgctcgcc cttgaccgcc gccagcagcg cctcgagatg
     7021 ttcggcgatc tccgggccgg gatcgatcac cgccacctcg cccgagccga cgatatagac
     7081 cccggtgccg gtataggtga acggccccgg attgcgggcg atcacccggc ggatcagcgg
     7141 cgagacctgg tcgcagcggc c
//
LOCUS       DAOMEO010000018         7054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1537_length_7054_cov_2.712102, whole genome shotgun sequence.
ACCESSION   DAOMEO010000018 DAOMEO010000000
VERSION     DAOMEO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_7054_cov_2.712102"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLH31_00810"
     CDS             complement(<1..451)
                     /locus_tag="PLH31_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQR87822.1"
                     /translation="MDIQLVAEGLLFPEGPIAMADGSVILTEIQGQRISRITPDGQRE
                     TVAETGGGPNGAAIGPDGALYVANNGGSFQFLDMGGLNVPGPTPPSHTGGSIQRVDLA
                     TGAITTLYTECDGKALVGPNDLVFDRQGGFWFTDHGCSTPEGRKFGAL"
     gene            complement(553..>1429)
                     /locus_tag="PLH31_00815"
     CDS             complement(553..>1429)
                     /locus_tag="PLH31_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR87823.1"
                     /translation="AEAKGLRLTMTLHGDDAQQPAVFALDEVRLGQVLLNLLSNAVKF
                     TTVGEVSARMALDPSTGTIRFEIDDTGIGIAPEVQSRLFQRFSQADSSISRSYGGAGL
                     GLAISKALIGQMGGQIGVESVPDLGSRFWITLPATPVETRQAPAVVDQNTLVPAAFRI
                     LLVDDHPMNRELGHALLTLAGCVVTTAEDGAQAVDAARDGGFDLILMDVHMPGMDGLA
                     ATRAIRSLPGAVAKTPIIALSADVMPEQIARCRLAGMNDHVAKPIHRETLIAAVMRAL
                     PLPETAAPVAALGAS"
     assembly_gap    1430..1439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1440..2599)
                     /locus_tag="PLH31_00820"
     CDS             complement(<1440..2599)
                     /locus_tag="PLH31_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR87824.1"
                     /translation="MPQGLNIDRVLVNRRRLIGLSIAYAGCMGYSLLLAGATHGLPTI
                     WTANAVLVAGLLVMNRKDRWTFLAVTAVLHVLVELPTGSPLPFLLLLSVLDTIQAATI
                     AWVLGMLRLPSRVRDMRSLTLLVAISALLTTVSSVLISGLLAVQNGRTFWTGWNDWTA
                     PNVLGMALALPTVLILLDTRLMRGFEVRSLEAAALALIAALTAAAIFSTDPLLQSLLF
                     APALLAVFRGGPRAVAVVITASLAATIPAVLHKTGVDPVLALPPLRQALTFHIVLYAV
                     CMVAALALSRQARLKTLLLRGQALARAAQARAIAANAAKSDFLATISHEIRTPLNSVL
                     GFAGLVADDPGLSAESRHRLDLINRAGRSLAEIVNDLLDFAKVEAGRIDLNLA"
     gene            complement(2696..3844)
                     /locus_tag="PLH31_00825"
     CDS             complement(2696..3844)
                     /locus_tag="PLH31_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQR87825.1"
                     /translation="MDAAGGAVTGGLIGSALEKPEGKAGEAHSGACTDCSQPVSGNFC
                     AHCGQPTHVHRSLLHLGEELLHGVMHFDGRIWRTVPLLVLNPGRLTREWVEGKRSRYV
                     SPLGLFLFTIFLMFFILSFGGGKAFETGAVNTRLDVARETVTDRHQEVAQAKTDLAVA
                     ERNVATAKPPQAEAAKAELDKAKSNFDDAVDDLADASNLVTRLEGVKAAGPRADGLEP
                     GSWQADVHDAVANDRVKVNMGGDGINKKVLKKLSNPDLALYKIQQTLYKFSFLLVPLS
                     IPFVALLFLWKRGFTLYDHGVFVLYSLTFMAMMSMALSFLGQISWLSSIVALIALIAP
                     PVHMFAQLKGAYGLSTFSAAWRTLLLLIFCAIALALFLAAIILLGLGG"
     gene            complement(3944..4456)
                     /locus_tag="PLH31_00830"
     CDS             complement(3944..4456)
                     /locus_tag="PLH31_00830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQR87826.1"
                     /translation="MIPILSIVVARASNGVIGRDGDLPWRLKSDLALFKANTLGKPII
                     MGRKTWDSLPRKPLPGRMNVVLSRDGSFEPANAVVCESFLEAVQMAREQAQEDGADEV
                     CIIGGRALFDMALPKAKRLYLTEVDAEVEGDVTFPAFDEAAWTEVRREEHPAGEGDDY
                     AFIFRVLERR"
     gene            4551..5573
                     /locus_tag="PLH31_00835"
     CDS             4551..5573
                     /locus_tag="PLH31_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR87827.1"
                     /translation="MTLSAFEMHMARTGGVEVLQGRSVALDPPGAGEARVAIEAAGVA
                     FADIVMRTGLYPGVKAPVTPGYDLIGRIEALGPGVEGFTVGQRVAALTVTGSYADRRN
                     VQARYLVAAPEGVAAETLVAGVLNGLTAWQMFHRVTSAQAGEWVLVHGAAGGVGGLLL
                     DLARLAGVRAIGTASAGKRAVVEAKGGVHVDYRSEPVAERARALSGGGVVAAFDHVGG
                     PHLKSASIASLRDGGTAVLYGGYDATRGGKMRPGAMIRMMMADRLSALKLFSASQGVV
                     GYNVTSWRDARPAAYRQDLAAVMAMIGEGQLKPQIGATFPLAEAAKAQELLQSAGVAG
                     KIVLKP"
     gene            complement(5578..6192)
                     /locus_tag="PLH31_00840"
     CDS             complement(5578..6192)
                     /locus_tag="PLH31_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87828.1"
                     /translation="MAFDRSVFINCPFDDAYQPLLRAILFCIYYLGLEPRIAVERLDG
                     AEPRIEKIIELIEASKFGIHDLSRIRAERAGELFRLNMPLELGLDIGCKRFKGRQWSE
                     KRCLILETERYRYQAAISDLSNSDIEAHGDDPAEALTIVRNWLNTACELDAEGPSFIW
                     GRFIDFISETDDVLVARGYSRRDIDRLPMVEWMRRMAAWDGMGK"
     gene            complement(6201..6425)
                     /locus_tag="PLH31_00845"
     CDS             complement(6201..6425)
                     /locus_tag="PLH31_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HQR87829.1"
                     /translation="MSKGQHVVPSIRGGWSVRATGAARASKTFPTQEAAIKHGKALAE
                     KEGAELYIHRADGTIRSRNSYGNDPFSPKG"
     gene            complement(6467..>7054)
                     /locus_tag="PLH31_00850"
     CDS             complement(6467..>7054)
                     /locus_tag="PLH31_00850"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQR87830.1"
                     /translation="IAWLKENGVRIWDEWADENGDLGPVYGKQWRSWATPNGGSIDQI
                     QNLIEGLKTNPNSRRHIVSAWNPADVEDMALPPCHCLFQFFVADGKLSCQLYQRSADV
                     FLGVPFNIASYALLTLMVAKVVGLEPGEFVHTFGDAHLYLNHLDQAREQLTREPFPFP
                     TLTIADKRDLFGFEYADFTLEGYEAHPHIKAEVSV"
BASE COUNT         1208 a         2243 c         2416 g         1177 t
ORIGIN      
        1 agagcgcgcc gaacttgcgg ccctcgggcg tgctgcagcc atggtcggtg aaccagaacc
       61 cgccctgcct gtcgaacacc aggtcgttgg ggccgaccag ggccttgccg tcgcattcgg
      121 tatagagcgt ggtgattgcg cctgtcgcca gatccacccg ctggatcgag ccgcccgtgt
      181 ggctgggcgg ggtgggaccc ggcacgttca ggccgcccat gtcgaggaac tggaagctgc
      241 cgccgttgtt ggcgacatag agcgccccgt ccggtccgat cgccgcgccg ttggggccgc
      301 cgccggtctc ggccaccgtc tcgcgctggc cgtctggcgt gatgcggctg atccgctgcc
      361 cctggatctc ggtgaggatc accgagccgt cggccatggc gatcgggccc tctggaaaca
      421 gcaggccttc ggcgaccagt tggatgtcca tcacgtctct ccctgcgcgc cttgacctgc
      481 cccttggagg ctggctccgt ctggtgcggg gcgcgaacga aaaggactat aaacgccggt
      541 ttggacgcgg ggctaggatg ccccaagggc agcgaccggc gccgctgtct ccgggagcgg
      601 cagggcccgc atcacggcgg cgatcagggt ctcacggtgg ataggcttgg cgacatggtc
      661 gttcatgccc gccaatcggc agcgcgcgat ctgctcgggc atgacgtcgg cgctcagggc
      721 gatgatcggc gttttggcca cggcgccagg cagggaccgg atggcgcggg tcgcggccag
      781 gccgtccatg cccggcatgt gcacgtccat caggatcagg tcgaagccgc cgtcgcgcgc
      841 cgcgtccacc gcctgcgcgc catcctcggc cgtggtgacg acgcagccgg ccagggtcag
      901 cagggcatgg cccagctcgc ggttcatcgg atggtcgtcg accagcagga tgcggaaggc
      961 cgccgggacc agcgtgttct gatcgaccac agcgggcgcc tgacgggtct cgacgggcgt
     1021 ggccggcagg gtgatccaga accgtgaccc caggtccggt acgctttcaa cgccgatctg
     1081 tccccccatc tgaccgatca gagccttgct gatcgccagg cccaggcctg cgccgccgta
     1141 gctgcggctg atcgaactgt cggcctggct gaagcgctgg aacaggcgtg actgcacttc
     1201 gggcgcgatg ccgatgccgg tgtcgtcgat ctcgaagcgg atcgtgcccg tcgacggatc
     1261 cagggccatg cgggccgaga cctcgccgac ggtggtgaac ttcacggcgt tggacagcag
     1321 gttcagcagc acctggccaa gccgcacctc atcgagggcg aagactgcgg gctgttgcgc
     1381 atcgtcgcca tggagggtca tggtcaggcg aaggcccttg gcctcggccn nnnnnnnnng
     1441 ccaggttgag atctattcgg cccgcctcga ccttggcgaa gtccagcagg tcattgacga
     1501 tctcggccag ggatcgcccg gcgcgattga tcaggtccag tcggtggcgg ctctcggccg
     1561 aaaggccggg gtcatcggcg accaggccgg cgaagcccag aacgctgttg agcggggtgc
     1621 gaatctcatg gctgatcgtc gccaggaagt cggacttggc ggcgttggcg gcgatggcgc
     1681 gggcctgggc ggcccgagcg agggcctgcc ctcgcaaaag cagggtcttc aaccgggcct
     1741 gacggctgag cgccagggcc gccaccatgc agaccgcgta gaggacgata tggaaggtca
     1801 gggcctgtcg caggggcggc agggccagga cgggatccac gccggtcttg tgaagcaccg
     1861 ccgggatggt ggcggccagg gaggcggtga tgaccacggc caccgcgcgc ggcccgcccc
     1921 ggaaaaccgc cagcagggcg ggcgcgaaca acagggattg cagcagcggg tcggtcgaga
     1981 agatggcggc ggcggtcagg gccgcgatca gcgccagggc ggcggcctcc agcgaccgca
     2041 cctcgaaacc gcgcatcagg cgggtgtcga gcaggatcaa gaccgtgggc agggccaggg
     2101 ccatgcccag cacattgggc gccgtccagt cgttccagcc cgtccagaac gtccgcccgt
     2161 tctggacagc cagtaacccg ctgatcagca ccgaggaaac tgtggtcaac agcgccgaga
     2221 tggccaccag caaggtcagg ctgcgcatgt cgcgcacccg gctgggcagc cgaagcatcc
     2281 ccagcaccca ggcgatggtc gccgcctgga tcgtgtccag cacggagagc agaagcagga
     2341 acggcagggg gctgccggtg ggcagttcga ccagcacatg cagcacggcg gtcacggcca
     2401 gaaacgtcca gcgatccttg cggttcatga ccagcaggcc cgcgaccagg accgcgttgg
     2461 ccgtccagat cgtgggcagg ccatgggtcg cccccgcgag cagcaggctg tagcccatgc
     2521 acccggcgta ggcgatcgag aggcctatga gcctgcgccg attgaccagc actctgtcga
     2581 tattcaaacc ttggggcacg caactgctct ctaaccgccg cgcaacctcg cgcgaaatcg
     2641 tcggcttttg gttaaggcgg tgtagcggcg cggtgtcgcg ggcggctaga ctcgactagc
     2701 cgcctaggcc gagaaggatg atcgccgcca ggaacagggc cagggcgatg gcgcagaaaa
     2761 tcagcagcag taaagtgcgc cacgcagccg aaaacgtgga aaggccataa gcccccttca
     2821 gttgggcaaa catgtgaacg ggcggtgcga tcagggcgat cagggccacg atgctgctca
     2881 accacgagat ctggcccaga aaactcagcg ccatgctcat catcgccatg aaggtcagcg
     2941 aatacagcac gaatacgccg tgatcataca gcgtgaagcc gcgcttccaa aggaacagca
     3001 gcgccacgaa aggtatggaa aggggcacaa gcaggaacga gaacttgtag agtgtttgct
     3061 ggattttgta gagcgccaga tcgggattgg agagtttctt caggaccttc ttgttgatcc
     3121 cgtcgccacc catattgacc ttgaccctgt cgttcgccac ggcgtcgtgc acgtcggcct
     3181 gccagctgcc cggttccaga ccgtccgccc gggggccggc cgccttgacg ccttccaggc
     3241 gggtgacgag attgctggcg tcggccaggt cgtcgacggc gtcatcgaag ttcgacttcg
     3301 ccttgtccag ctccgccttg gcggcttcgg cctggggtgg cttggcggtc gcaacgttac
     3361 gctccgcgac ggcgaggtct gtcttggcct gggccacctc ctgatgcctg tccgtgacgg
     3421 tctcacgcgc cacgtccagg cgcgtgttga cggcgccggt ttcgaaggcc ttgccgccgc
     3481 cgaagctgag gatgaagaac atcaggaaga tggtgaacag gaacaggccc aggggcgaca
     3541 catagcgcga ccgcttgcct tccacccatt cacgcgtcag gcggcccggg ttgagcacca
     3601 gcaagggcac ggtgcgccag atccgcccgt cgaagtgcat cacgccatgc agcagttcct
     3661 cgcccaggtg cagcagcgag cggtgcacat gggtgggctg gccgcaatgg gcgcagaaat
     3721 tgccgctgac cggctggctg caatcggtgc aggcgccgga atgggcctcg cccgccttgc
     3781 cctcgggctt ttccagcgcg ctgccaatca ggccgccggt gactgccccg ccggctgcat
     3841 ccatgtccac catccgtcgc gccccgtttg cccgcgcctc ctgtgccttg gcagcgcgcg
     3901 accgtcaaaa gattagctgc gccttgcaca cgcgccgatg gttttagcgc cgctccagca
     3961 cccggaagat gaaagcgtag tcatcgccct cgccggcggg gtgttcctcg cggcggactt
     4021 cggtccaggc ggcctcgtcg aacgcgggga aggtcacatc gccctcgacc tcggcgtcca
     4081 cctcggtgag atagagccgc ttggccttgg gcagggccat atcgaacagc gcccggccgc
     4141 cgattatgca gacctcgtcc gcgccgtctt cctgcgcctg ctcgcgggcc atctgcacgg
     4201 cctccagaaa actctcgcag acgacggcgt tggcgggctc gaagctgccg tcgcgcgaca
     4261 gcaccacgtt catgcgaccg ggcaggggct tgcggggcag gctgtcccag gtcttgcgac
     4321 ccatgatgat cggcttgccc agggtgttgg ccttgaacag ggccaggtcc gacttcagcc
     4381 gccatggcag gtcgccgtcg cgaccgatga cgccattgga ggcgcgggcg acaacgatgg
     4441 aaaggatcgg gatcatggcc ctgcttaacc ttaccccctc aaggacgcca gccaagagac
     4501 cttgccagtt atctgaacgc cgttaacttg gcgtaacggg agacagcagc atgaccctat
     4561 cggccttcga gatgcacatg gcccgcactg gcggcgtcga ggttctgcag ggacgctccg
     4621 tcgcgctgga tccccccggc gcgggcgagg cgcgggtggc gatcgaggcg gccggcgtgg
     4681 ccttcgccga catcgtcatg cgcacggggc tctatcccgg ggtcaaggcg ccggtgacgc
     4741 ccggctatga cctgatcggc cggatcgagg ctctgggacc gggtgtcgag ggattcactg
     4801 tgggccagcg cgtcgccgcc ctgaccgtga cgggctccta tgccgaccgc cgcaacgtgc
     4861 aggcgcgcta tctggtcgcc gcgcccgaag gcgtcgccgc cgagaccctg gtggccgggg
     4921 ttctgaacgg cctgacggcc tggcagatgt ttcaccgcgt gacctcggcc caggcgggcg
     4981 agtgggttct ggtgcatggc gcggcgggcg gcgtcggtgg attgctgctg gacctggcgc
     5041 gcctggcggg cgtgcgggcc atcggcacag cctcggcagg caagcgcgcg gtggtcgagg
     5101 ccaaaggcgg ggtccatgtc gactatcgca gcgaacccgt cgccgaacgg gcccgcgccc
     5161 tctccggcgg cggcgtggtc gccgctttcg accatgtggg cgggccgcac ctcaagagcg
     5221 cctcgatcgc atccctgcgc gatggcggaa cggcggtgct ctatggcggc tatgacgcga
     5281 cccggggcgg caagatgcga cccggcgcga tgatccggat gatgatggcc gaccggcttt
     5341 cagcgctgaa gctgttctcg gccagccagg gtgtggtggg ctacaacgtc acctcatggc
     5401 gcgacgcgcg gccggccgcc tatcggcagg atctggcggc ggtgatggcg atgatcggcg
     5461 agggtcagct caagccgcag atcggcgcga ccttccccct ggccgaggcg gccaaggccc
     5521 aggaactgct gcagtcggcc ggggtggccg ggaagatcgt gctgaagcct taggtggcta
     5581 cttacccatt ccgtcccatg ccgccatacg tcgcatccat tcaaccatgg gcaggcggtc
     5641 gatatcacgt ctggagtagc cgcgagcgac aaggacgtcg tccgtttccg agatgaaatc
     5701 gatgaaccgg ccccagatga aggatgggcc ttcggcgtcg agttcacacg cggtgttcaa
     5761 ccagttgcgg acaatggtca gagcttcggc tgggtcatcg ccgtgagcct ctatgtcgga
     5821 gtttgagaga tccgagatcg ccgcctggta gcggtagcgc tcagtctcaa ggatcaagca
     5881 tctcttctcc gaccattggc ggcccttaaa ccgcttgcag ccgatgtcca agcccagttc
     5941 aaggggcatg ttcagcctga acaactcccc ggcccgttca gcgcggatac gggaaaggtc
     6001 gtggatgccg aacttcgagg cttcaatcag ctcgatgatc ttctcgatgc gcggttcggc
     6061 tccatcgagt cgttcgaccg cgatacgcgg ctccagaccc aagtagtaaa tgcagaatag
     6121 aatagctctg agcagcggct ggtaggcatc atcaaaaggg cagttgatga agacgctacg
     6181 gtcgaacgcc atgggatggc tcaacccttt ggcgaaaagg gatcgttgcc gtagctgttt
     6241 cgcgagcgga tggtgccgtc cgcgcggtgg atgtagagtt cggcgccttc cttttcagca
     6301 agcgcctttc catgtttgat cgccgcctcc tgggtaggaa acgtctttga agcgcgagct
     6361 gctcctgtgg ctcggacgct ccagccaccg cggatagacg ggacaacatg ttgtcctttg
     6421 gacatcggac tctccttggt agatgcccaa tatagcgcgg ccgaccctag accgaaacct
     6481 cggccttgat atgcgggtgg gcttcgtagc cttccagcgt gaagtccgca tactcgaacc
     6541 cgaacaggtc gcgcttgtcg gcgatggtca gggtggggaa ggggaagggc tcgcgggtca
     6601 gctgctcgcg ggcctggtcc aggtggttca gatagaggtg cgcgtcgccg aaggtgtgga
     6661 cgaactcgcc gggctccagt cccaccacct tggcgaccat cagggtcagc agggcgtaac
     6721 tggcgatgtt gaacggcacg cccaggaaga cgtcagcgct gcgctgatag agctggcagg
     6781 agagcttgcc gtcagccacg aagaactgga acaggcagtg acagggcggc agggccatgt
     6841 cctcgacatc ggccgggttc caggcgctga cgatgtggcg gcggctgttg ggattggtct
     6901 tcaggccctc gatcaggttc tggatctggt cgatcgaacc cccgttcggc gtcgcccacg
     6961 agcgccactg cttgccatag acgggaccca gatcgccgtt ctcgtcggcc cactcgtccc
     7021 agatgcgcac gccgttttcc ttgagccagg caat
//
LOCUS       DAOMEO010000019         6899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1569_length_6899_cov_2.459527, whole genome shotgun sequence.
ACCESSION   DAOMEO010000019 DAOMEO010000000
VERSION     DAOMEO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_6899_cov_2.459527"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLH31_00855"
     CDS             complement(<1..214)
                     /locus_tag="PLH31_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87831.1"
                     /translation="MTDPTDCKICGMLHAAAPEDVVLDGPHWGVVAMQDVPGMLMAFT
                     RAHDRGMGSLSDAAAAELGPLINRLSA"
     assembly_gap    362..371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<372..1309)
                     /locus_tag="PLH31_00860"
     CDS             complement(<372..1309)
                     /locus_tag="PLH31_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR87832.1"
                     /translation="MLIDLHAHAPLPGYYNQDPHWGPFFEKHDDGHIRLRVGEWILTL
                     GSPERRAAVKRGETMEVAEFMSKWNDPKHRLAGMDALGQDAQVLSVPSHCYMYWADPA
                     FANRFSSTVNDTLADYCDRSGGRLMMWATAALQDPVNAAKEIRRACTELGAKGLSAGG
                     SNFGGMEFDDPRMDPIWETLCDLDLPMFVHGYNQSVTWGNKAIVGMNYDETKCFWYLI
                     CGGVLDRFPNLKVYITHAGGFVPYQLGRLAQTIKNLDVVHNKKDLEAYMPNFWFDPEL
                     HELPMRQALVEVIGADRLLYGTNFGGSDAIRGDLTAG"
     assembly_gap    1586..1595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1596..2862
                     /locus_tag="PLH31_00865"
     CDS             <1596..2862
                     /locus_tag="PLH31_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR87833.1"
                     /translation="TLLSQLDRQLPALLVRPIRAEFGISDTAFSFLQGYAFALFYTFA
                     GLPFGWLIDRTVRRNLIIVGMVLWSLMTVLSGFAQSYEQLVMSRMGVGIGEAVLAPAA
                     YSIIADYVAPERRGRAFSVYYLALAIGSGASLILGALIARIVPDAGLSLPGVGLMSQW
                     RLTFLIAGAPGLLLALLLLTIREPVRRDAAVLLSKAGASWGDFLSHLKRHLATYSRVL
                     TYPGVVAVVGYGTLAWAPTFFDRRFDIPTKTSGLIIGVLVAAGGLTGTLISGWISDRW
                     TAKGVPAARLRVAMLAWLLVLPTVCAWSLVGIPWLSFALLTVVITGFGMAQAAAPTAV
                     QEITPNRMRGKAVAVYLLIGGLVGIGFGPMSIALVTDHVFKNDAGLPYALAMVGGPIS
                     LLGLWLTWSGLKPYQRAVETLKAEAARPS"
     assembly_gap    2944..2953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2954..3908)
                     /locus_tag="PLH31_00870"
     CDS             complement(<2954..3908)
                     /locus_tag="PLH31_00870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR87834.1"
                     /translation="MLIDVHAHLFTPGMLNRHPFWGPFMKPQGLTVGHFSLGTKQPAK
                     AANDAEAEANLLARMTHSARLAVMEQRGVDKLVLSTPSHAFMYWAGEFGNEYAKICND
                     ELSAFCRVMPDKFDFWAHANLADPVAAVDEIDRAVRVLGAKGLCVGGTNFDGIQTYDE
                     RLFPVWAKLAELDVPIMVHGFNQSIYLGERHHEDRFETTSIVGDCVDETLFFWYLICG
                     GALDAFPTLKTYISHAGGMAVFQLGRLSELNGAMAPDAKNQKPLMDYMSNFYFDLDVH
                     HPALRRAVAEVVGADRLLYGTNFGGAYDHGDLTAGIDLSDID"
     gene            4017..5234
                     /locus_tag="PLH31_00875"
     CDS             4017..5234
                     /locus_tag="PLH31_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR87835.1"
                     /translation="MSQQQSERGGRLLLAAGIVGTAFCLRLVFASLSVRLPEVVGATG
                     LAPWQVGLLTTLPVLCLGVCAPMAPQLARRWGIERSLFGALALVGLGTAGRALGPVWA
                     LFSFSILAGAAIATANVLLPTLVKRDFEDRTALLTGAYVTAISGGAALAAAVTVPIEA
                     ALGGDWRVGLSMWTLPVAGALLLWLPQIRGRERQGAGIVFPVSGLWRDPLAWSVALFM
                     GLQSALAFCVLGWLAPILRERGLDAVTAGLVLSVLIVVQLATSLAVPSIAARRPGQRV
                     IAVVLSVAGAAGLLGLLLAPLSGVWVWAVVQGLAQGGLFSLALTMVLLRSPDSHVASH
                     LSGMAQSVGYVPAALAPLGVGLLHQWTGGFEAVAGLVALIGIGLVVTGLIAGRAAVVM
                     ARAAPIHGELARA"
     gene            5231..6037
                     /locus_tag="PLH31_00880"
     CDS             5231..6037
                     /locus_tag="PLH31_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR87836.1"
                     /translation="MTGAIAQEADAMRFTNRVAIVTGAGGGIGKVTAQLLAAQGAVVA
                     VNDLNEAAAQASVDDIRSSGGQAFVLQGDAADEDFAIEAVAEVMRSQGRIDILVNNAG
                     VSVVAPAEDFVAWDRVLRTNLYSQFFWSKAVAKAAMIPAARGAIVNVASIAGLAAVPN
                     QIGYVVSKHGVVGLTRSLAVEWGRHGIRVNCVCPGITATDLTTGPSGMDADRLSARIA
                     RVPLGRMGTAREQAEAIAFLVSDEASYLSGLILNNDGGQLALHSGVMMSA"
     gene            6207..6683
                     /locus_tag="PLH31_00885"
     CDS             6207..6683
                     /locus_tag="PLH31_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR87837.1"
                     /translation="MYRRIVLAYDSTEAGRAALREGALLAKRCGAEVFLLSVLTDSGG
                     LALAEGAHAGAAHGHLEAYGAVLNEGLRKLERLGFTPTGKLVRGEPAQQIGDYAREVG
                     ADLVVVGHRKRSLLERWWSGTNGAYIVDNLDCSLLVARSDISNEAFEAELSQAARP"
     gene            complement(6691..>6899)
                     /locus_tag="PLH31_00890"
     CDS             complement(6691..>6899)
                     /locus_tag="PLH31_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-carotene ketolase"
                     /protein_id="HQR87838.1"
                     /translation="RTSLTKGCVTHVDFGAQRTGAKRPAFGLGGPEPLAPGFFLGGAG
                     IHPGGGVSGGPGRLVSKRVLDYLA"
BASE COUNT         1034 a         2183 c         2334 g         1318 t
ORIGIN      
        1 tggcagaaag gcggttgatc agcgggccga gctcggccgc cgccgcgtcg gagagcgatc
       61 ccatgccccg gtcatgggcg cgggtgaacg ccatcagcat gccgggcacg tcctgcatgg
      121 cgacgacgcc ccagtgcggg ccgtccaaca cgacgtcttc cggcgcggcg gcgtgaagca
      181 tgccgcagat cttgcagtcg gttggatcgg tcatgggcgt cctgaacgtc gcgccaaagg
      241 tgcgcggcgg cccccgaggg accgccgctc aacggaaagt taggccgcga tcggcttgtt
      301 cgcccgaccc atcttgtcca ggtcgatgtg cagcagatcg accgcattct tccagcggat
      361 cnnnnnnnnn nccagccgtc aggtcgccgc ggatcgcgtc gctgccgccg aaattggtgc
      421 catagagcag gcggtccgcg ccgatcacct cgaccagggc ctggcgcatc ggcagctcgt
      481 gcagctccgg atcgaaccag aagttcggca tatacgcctc caggtccttc ttgttgtgga
      541 caacgtcgag gttcttgatg gtctgggcca ggcggcccag ctgataaggc acgaagccgc
      601 cggcgtgggt gatgtagacc ttcaggttgg ggaagcggtc gagcacgccg ccgcagatca
      661 aataccagaa gcatttggtc tcgtcgtagt tcatgccgac gatggcctta ttgccccagg
      721 tcacggactg gttgtagccg tgcacgaaca tcggcaggtc gaggtcacac agggtttccc
      781 agatcgggtc catgcgcgga tcgtcgaact ccatgccgcc gaagttggaa ccgccggcgc
      841 tcaggccctt ggcgcccagt tcggtgcagg cccggcggat ttccttggcg gcgttcaccg
      901 ggtcctgaag cgcggcggtg gcccacatca tcagccggcc gcccgagcgg tcgcaatagt
      961 cggccagggt gtcattgacc gtgctggaga accggttggc gaacgccgga tcggcccagt
     1021 acatatagca gtgcgaaggc accgacagca cctgggcgtc ctgacccaag gcgtccatgc
     1081 cggccaggcg gtgctttggg tcgttccact tggacatgaa ctcggcgact tccatcgtct
     1141 cgccccgctt gaccgcggcg cgacgctccg gcgaaccgag ggtcaggatc cattcgccga
     1201 cccgcaggcg gatatgtccg tcgtcgtgct tctcgaagaa gggaccccag tgcggatctt
     1261 gattgtaata gcccggcagc ggggcatggg cgtgaagatc gatcagcatc tgacttcctc
     1321 ctgcgtcgag gccccctggc ggggctcttc gagccataat acgttccctg acgacgacta
     1381 tcgcatttta tgaacgaatt cgtcgatagt gttcagtcaa ttctctaacg cgaatggcgt
     1441 tcgcttgtca aaacaaccct tgacagcctt gaggctccag cgggtaacgt caggtcgcgt
     1501 cctcgtccct gcgagcgtga aataattgag gaaggcgcgt tcttcggtga cgttgccgtt
     1561 ctcgatgagc gcgccgcgca gggagnnnnn nnnnngaccc tgctttcgca actggaccgg
     1621 cagttgccgg cgctgctggt gcggccgatc cgggccgagt tcggcatttc cgacaccgcg
     1681 ttcagcttcc ttcaaggcta cgctttcgct ctgttttaca ccttcgcggg actgcccttc
     1741 ggctggctga tcgatcgcac ggtccgtcga aatctcatca tcgtcggcat ggtcctgtgg
     1801 agcctgatga cggtcctgtc cggcttcgcg caaagctacg aacaactggt gatgagccgg
     1861 atgggcgtcg gcatcggcga ggcggtgctg gcgccggccg cctattccat catcgccgac
     1921 tatgtcgcgc ccgagcggcg cggacgcgcc ttcagcgtct actatctggc cctggcgatc
     1981 ggttcggggg cgtcgctgat tctgggggcg ctgatcgccc gcatagtccc ggacgccggg
     2041 cttagcctgc cgggcgtcgg gctgatgtcg caatggcgcc tgacgttcct gatcgcgggc
     2101 gcccccggtc tcctgctggc gctgctgttg ctcacgatcc gcgagccggt gcgtcgcgat
     2161 gctgcagttc tgttgtcgaa ggcgggcgcg agctgggggg actttctgtc ccacctgaaa
     2221 cgccacttgg ccacctattc gcgggttctg acttatcccg gtgtcgtcgc cgtcgtcggc
     2281 tatggcacgc tcgcctgggc gccgactttc ttcgaccggc ggtttgatat ccccaccaag
     2341 acgtccggcc tgatcatagg cgtgcttgtc gcggccggcg ggctgaccgg cactctgatc
     2401 agtggctgga tcagcgatcg ttggaccgcc aagggcgttc ctgccgcgcg gctgcgggtg
     2461 gccatgctgg cctggctgct ggtgctgccg acggtttgcg cgtggtcact ggtgggcatc
     2521 ccctggctga gcttcgccct gctgaccgtg gtcatcacag gctttggcat ggcccaggcg
     2581 gcggcgccga cggcggtcca ggagatcacc cccaaccgga tgcgcggcaa ggcagtggcc
     2641 gtctatttgc tgatcggcgg tctggttggg atcggctttg ggcccatgtc gatcgctctg
     2701 gtgaccgacc atgtcttcaa gaacgacgct ggtttgccct acgccctggc catggtcggc
     2761 gggccgatct ctctgctcgg cctgtggctg acctggtcgg gcctgaaacc ctaccaacgc
     2821 gcggtcgaga ctctgaaggc cgaagccgcg cgaccgtcct gacgacgcgc ggcctcggag
     2881 gcttcaggcg acccgggcgg ccttgcccac gtcgagcttg agcagttcga tggcgttgcc
     2941 gctnnnnnnn nnnggtcgat gtccgacagg tcgatgccgg cggtcaggtc gccgtggtcg
     3001 taggccccgc cgaaattggt gccgtacaac agccggtcgg ctcccacgac ctcagccacc
     3061 gcgcggcgca gggccgggtg gtggacgtcc aggtcgaagt agaagttgga catatagtcc
     3121 atcagcggct tttggttttt cgcgtcgggc gccatagcgc cgttcagttc cgacagtcga
     3181 ccgagctgga acaccgccat gccgccggcg tggctgatgt aggtcttcaa ggtcgggaag
     3241 gcgtccagcg cgccgccgca gatcagatac cagaagaaca gggtctcatc gacgcagtcg
     3301 ccgacgatgg aagtggtctc gaaccggtcc tcgtgatggc gctcgcccag atagatcgac
     3361 tggttgaacc catgcaccat gatcggaacg tccaactccg ccagcttggc ccagaccgga
     3421 aacagtcgtt cgtcataggt ctggataccg tcgaagttcg ttccgccgac gcacaggccc
     3481 ttggcgccca gcacgcgcac ggcgcggtcg atctcgtcca cggcggcgac cggatcggcc
     3541 agattggcgt gggcccagaa atcgaacttg tcgggcatca cgcggcagaa ggccgacagt
     3601 tcgtcgttgc agattttcgc gtactcgttc ccgaactctc cggcccagta catgaaggcg
     3661 tgcgacggcg tggacagcac cagcttgtcg acgccgcgtt gctccatgac cgccagcctg
     3721 gccgaatgcg tcatccgggc cagcaggttg gcctcggctt cggcgtcgtt cgccgccttg
     3781 gcgggttgtt tcgtgccgag cgagaaatgc ccgaccgtca atccctgcgg cttcatgaac
     3841 ggaccccaga acggatgccg attcagcatg ccgggtgtga agaggtgggc gtgcacgtcg
     3901 atcagcatgg gcttcctcgt ttatctgtta ttagattgat attctcactg tcgaatggtc
     3961 tgtgacaagt cgcatcggca tgatcatttc cgacgcgggg aatcgaaggc gaaacgatgt
     4021 cgcagcaaca gagcgaacgc ggcggccggc tcctgctggc ggccggcatt gtcgggacgg
     4081 ctttctgcct gcgcctggtg ttcgcgagcc tgtcggtgcg cttgcccgag gtcgtcggcg
     4141 cgacagggct cgcgccctgg caggtcggac tgctgacgac cctgccagtg ctgtgtctcg
     4201 gcgtttgcgc gccaatggcc ccccagttgg cgcggcgatg gggaatcgag cgcagcctgt
     4261 ttggcgcgct ggcgctggtc ggattgggaa cggcggggcg cgcgctcggg ccggtctggg
     4321 cgctgttctc gttctccatt ttggccggag ccgcgatcgc cacggccaat gtgctgctgc
     4381 cgactttggt gaagcgtgat ttcgaggacc ggacggcctt gctgaccggc gcctatgtca
     4441 cggcgatttc cggtggcgcg gccctggccg ccgccgtcac cgtaccgatc gaggccgcgt
     4501 tgggcggcga ctggcgggtc ggactgtcga tgtggactct accggtcgct ggcgcgctgt
     4561 tgctctggct gccccagatc cgtggtcgcg agaggcaagg agccgggatc gtctttccgg
     4621 tctccggcct ttggcgcgat ccgctggcgt ggagcgtggc cctgttcatg gggctgcagt
     4681 cggccctggc gttctgtgtc cttggctggc tggcgccgat cctgagagaa cggggactgg
     4741 atgcggtgac tgcgggactg gttttatcgg tcttgatcgt ggtccagctg gcgacctcgc
     4801 tggccgttcc ctccatcgcc gcccgcaggc cgggccagcg cgtgatcgcg gtcgtcttgt
     4861 cggtcgcagg cgcggccgga ttgctgggcc ttctgcttgc cccactatca ggcgtctggg
     4921 tctgggcggt cgtccagggt ctggcccagg gtggcttgtt ctcgcttgcg ttgacgatgg
     4981 tgctgctgcg ctcgcctgac agccatgtcg cctcccatct gtcgggcatg gcccagagcg
     5041 tgggctatgt cccggccgcg ctcgcgccgc tcggtgtcgg gcttcttcat cagtggaccg
     5101 gcggattcga ggccgtcgcg ggcctggtgg cgctgattgg aatcggtctg gtcgtcaccg
     5161 gcttgatcgc cggtcgagcg gccgtggtca tggcccgagc cgcgccaatc cacggcgaac
     5221 tggcgcgggc atgaccgggg cgatcgctca ggaggcagac gccatgcggt tcaccaacag
     5281 ggtcgccatc gtgaccgggg cgggcggagg catcggcaag gtgacggccc agctgttggc
     5341 cgcccagggc gccgttgtcg ccgtcaacga cctcaacgag gccgcggccc aggcgagcgt
     5401 cgatgacatc cgctcgagcg gaggccaggc cttcgtcctc cagggcgacg cggccgacga
     5461 ggatttcgcg atcgaggccg tcgccgaggt catgcggagc cagggtcgga tcgacattct
     5521 ggtcaacaac gccggtgtgt cggtggtcgc gccggccgaa gatttcgtcg cttgggaccg
     5581 cgtgctccgc accaatctct attcgcagtt cttttggtcg aaggccgtgg ccaaggcggc
     5641 catgatcccg gccgcccgag gcgcgatcgt caacgtggcg tccatcgccg gtctcgcggc
     5701 tgtccccaac cagatcggct atgtggtgtc caagcacggc gtcgtcggcc tgacgcgatc
     5761 cttggcggtc gagtggggcc ggcacggcat tcgggtcaac tgcgtctgtc ccggcattac
     5821 ggccaccgac ctgaccaccg gcccgagcgg catggacgcc gaccgcctca gcgccaggat
     5881 cgcgcgcgtc ccgctgggaa ggatgggtac ggcgcgggag caggcggaag ccatcgcttt
     5941 cctggtgtcg gacgaggctt cctatctctc gggcctgatc ctcaacaatg acggcggtca
     6001 gctggcgctc cactccggcg tcatgatgtc agcctgatcg gttcgaacac gtcgctggcg
     6061 ccaaagagcc ttcgatctgg gtgggcgaaa agctgaacaa gcttgccaat ttcgtccaag
     6121 attggaagat cggctccggg atcgcgccgg ctgtcctgcc gcgggcaggg tgggcggccg
     6181 acctcgcgac ggaataggaa gtcatcatgt atcggcgcat cgttctggcc tacgacagca
     6241 ccgaggcggg gcgcgcggcg ttgcgagaag gggctttgct ggccaagcga tgcggggccg
     6301 aggttttcct attgtcggtc ctgaccgaca gtggaggctt ggccttggcc gagggcgcgc
     6361 atgccggcgc ggcacatggt catcttgaag cctatggcgc cgttctcaac gaagggctgc
     6421 ggaagctgga acggttgggc ttcaccccga ctggcaagct tgtccgcggc gagcctgccc
     6481 agcagatcgg tgactacgcg cgagaggtcg gcgccgacct cgtcgtggtc ggccatcgca
     6541 agcgcagctt gctcgagcgc tggtggagcg gcacgaacgg cgcctacatc gtcgacaacc
     6601 tcgactgcag tctgctggtg gcgcgaagcg acatcagcaa tgaggccttc gaggccgaac
     6661 tttcccaagc cgcgcgaccg tgagggcggt tcaggccaga tagtcgagca cccgtttcga
     6721 caccaggcgt cccggccccc ccgagactcc gccgcccgga tggattcccg cgccgcccag
     6781 gaaaaacccc ggcgccagcg gctcgggtcc gcccagcccg aaggccggtc gcttcgcacc
     6841 cgttcgctga gcgccgaaat cgacatgggt gacgcagccc ttcgtcaggc tcgttctcg
//
LOCUS       DAOMEO010000020         6876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1577_length_6876_cov_2.775400, whole genome shotgun sequence.
ACCESSION   DAOMEO010000020 DAOMEO010000000
VERSION     DAOMEO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_6876_cov_2.775400"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLH31_00895"
     CDS             <1..294
                     /locus_tag="PLH31_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQR87839.1"
                     /translation="HRRAHSVDPHISIATVYRTVRLFEESGIIERHDFRDGRSRYEET
                     PSHHHDHLIDMKTGKVVEFVDEEIEALQNAIAQRLGYRLIDHRLELYGVPIEE"
     gene            382..1734
                     /gene="miaB"
                     /locus_tag="PLH31_00900"
     CDS             382..1734
                     /gene="miaB"
                     /locus_tag="PLH31_00900"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQR87840.1"
                     /translation="MSETPQKRLYIKTYGCQMNVYDSERMADVLRPLGYGVVDEPEGA
                     DLVVLNTCHIREKATEKVYSELGQIKLMKDRKAADGGRMTIAVAGCVAQAEGQEIMTR
                     QKAVDLVVGPQSYHQLPELIARAHRATGERLSTSFAADEKFDALPAERQVNGVTAFLT
                     VQEGCDKFCTFCVVPYTRGGEWSRPVNDILEEARRLADQGVREVTLLGQNVNAYDGDG
                     STLAKLVRQLAKIDGLDRIRYTTSHPRDMGDDLIEAHGELPELMPYLHLPVQAGSDKI
                     LKAMNRDHTAESYVRLIEKIRAARPDIAMSGDFIVGFPGERDGDFEKTLELVREVGFA
                     SAFSFKYSRRPGTPASAMTGQVDEAVKAQRLERLNQLLDEQQKAFNATQVGKVLPVLF
                     EKKGRHAGQIVGRSPYLQAVHAEGGDHLLGTIVPVRIDSAAKMSLGGVLETSAVLETA
                     "
     gene            1731..2675
                     /locus_tag="PLH31_00905"
     CDS             1731..2675
                     /locus_tag="PLH31_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR87841.1"
                     /translation="MSRTPEFLPLSDDAVHAVSGPGGRHAALIEDAFRVLIETPGGGV
                     TITGDVKGRTGAKRALNDLAARADAGEEVVEADVRIAVGEAQSTGGQASARTVRKGSV
                     SPKTKGQARYMEAMGSHPLSFGLGPAGTGKTFLAVAHGAGMLLRGEVDRLIVTRPAVE
                     AGEKLGFLPGDLNEKVDPYMAPVWEALTDIMGAEQLRRRREKLEIEVAPIAFMRGRTL
                     AHAFVIVDEAQNCSRLQMKMVLTRLGEGSRMVVTGDPTQIDLLNPRDSGLAHAVSILE
                     GVEGVSVSRFTAADVVRHPLVERIVKAYDADAAQSTPR"
     gene            2721..3191
                     /gene="ybeY"
                     /locus_tag="PLH31_00910"
     CDS             2721..3191
                     /gene="ybeY"
                     /locus_tag="PLH31_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQR87842.1"
                     /translation="MEIEDEAWLKAEPETEALVWRAAQAVLDAHQDIEGQGIVILLAD
                     DDSVQALNRDFRQKDYATNVLSFPSPQGPEANPEGQIGDIVLAFGVCQREAIEQGKPL
                     AHHLQHLVAHGVLHLLGYDHEGDEEAETMEALEREILATLDIPDPYAADVTEQG"
     gene            3203..4108
                     /locus_tag="PLH31_00915"
     CDS             3203..4108
                     /locus_tag="PLH31_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQR87843.1"
                     /translation="MPSDDSSPQPGGPARQSRGVRAFFRRMRKQLSGAAEPARPPEPV
                     QDGAVDIVDQAEAFKTLRVADVMTPRADIVSVELSTPFDALIAQFTEAEHSRMPIYRE
                     TLDDPVGVVHVKDVFRMLAAGEKRPSGTDVVLHKVRREALYVPASMRAADLLLRMRTS
                     RIHLALVIDEFGGTDGLVTMEDLIEAVVGDIDDEHDDATVAGIVARPGGVFEADARAP
                     LEELEAALGRELAPPDMEEDIDTVAGLVVALAGRVPQRGEVIAHPHGYDLEVVEADPR
                     RVRRVRVRPAAIPGAASQPAEAAAS"
     gene            4105..>5015
                     /locus_tag="PLH31_00920"
     CDS             4105..>5015
                     /locus_tag="PLH31_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQR87844.1"
                     /translation="MNLKLATPWRERGLALLAGLAAALAHPPFGVLPGLLGYALLLWL
                     LDTIDDPRPLRAAFFRGWLAGLGYFGLGTWWIGEAFLVDAANQGWMAPFAIAAMAGGL
                     ALFWGVAAVAYRLARPPGVRRVLVFAGAFAALEWTRGHVLTGFPWNLPGETWKAGSAP
                     SQAAAIVGAYGLTWITLAIAAAPAVWRDGLAGRKAIAAAAALLAALYINGWMPAAPAP
                     KTTTPKTTTPTVRIVQANIQQDLKWDEGRFVQIAQSYVDLTARPYKDGKPADIVVWPE
                     GALPLSLNEYLAPGVWVRDAIIDALQPG"
     assembly_gap    5016..5025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5026..5658
                     /gene="lnt"
                     /locus_tag="PLH31_00925"
     CDS             <5026..5658
                     /gene="lnt"
                     /locus_tag="PLH31_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQR87845.1"
                     /translation="RQDGTLQDPVYFNSLVALRRGADDIEVVGIYDKHRLVPFGEFLP
                     MDALLTDLGVKSMAHLGDGFETGPRPAPLQVAPDLIVQPLICYEALFPGLAKPDPRVR
                     ALVNVSNDAWFGVTSGPLQHLNLASYRAIETGLPIIRATPTGVSALIDARGKIVGGAQ
                     LNLGDAGVIDGVLPDMGTATPFGRLGNTPFILMILISAFAGVRLKVGKGF"
     gene            5722..6153
                     /locus_tag="PLH31_00930"
     CDS             5722..6153
                     /locus_tag="PLH31_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR87846.1"
                     /translation="MSAPSDQDRHPNPIDLHVGARIRMRRKILGVSQERLAEDLGLTF
                     QQVQKYERGANRVSASKLYEIARSLQSSVAYFFEGLADPTSAEGMAETGSEQFVHDFL
                     MTPEGLELASLFPKINRGKVRRRILDLVRSMADEETPEAED"
     gene            6264..>6876
                     /locus_tag="PLH31_00935"
     CDS             6264..>6876
                     /locus_tag="PLH31_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQR87847.1"
                     /translation="MSRSSYIFTSESVSEGHPDKVADRISDTVVDAFLSVDPEARVAC
                     ETLVTTNRIVLAGEVRAGKPDGDKAANKQLTKDILKSLEPKVRAAIKDIGYEQKGFHW
                     EKAKFANYLHGQSAHIAQGVDASDKKDEGAGDQGIMFGYASTETPELMPATLQYSHNI
                     LSHLARLRHSGELSALEPDAKSQVTLEYDANGKPTRVVSIVVSH"
BASE COUNT         1177 a         2354 c         2250 g         1085 t
ORIGIN      
        1 caccgccgcg cccactctgt tgacccgcac atctcgatcg ccacggtcta tcgcaccgtg
       61 cgcctgttcg aggagagcgg catcatcgag cgccacgact tccgcgacgg ccgctcgcgc
      121 tatgaagaaa cgcccagcca tcaccacgac cacctgatcg acatgaagac cggcaaggtc
      181 gtcgagttcg tggacgagga gatcgaggcc ctgcagaacg ccatcgcgca gcggctgggc
      241 tacaggctga tcgaccaccg gctggagctt tatggggttc cgatcgagga gtgatcgcgg
      301 cctaaagtcc tccccctgaa gggggaggac ttgcagcgcc gatcttgcgc cagcgccctc
      361 acgccctcat aacctcgccg gatgagcgag accccgcaaa agcgcctcta tatcaagacc
      421 tacggctgtc agatgaacgt ctatgacagc gagcgcatgg ccgatgtcct gcgtccgctg
      481 ggctatggcg tggtcgatga gcccgaaggc gccgatctgg tggtgctcaa cacctgccac
      541 atccgcgaga aggccaccga aaaggtctat tcggagcttg gccagatcaa gctgatgaag
      601 gaccgcaagg cggcggacgg cggtcgcatg accatcgccg tggccggctg cgtggcccag
      661 gccgagggcc aggagatcat gacccgccag aaggcggtgg atctggtcgt gggtccgcaa
      721 tcctatcacc agctgcccga gctgatcgcc cgcgctcacc gggccaccgg cgagcggctg
      781 tcgaccagct ttgccgccga cgagaagttc gacgccctgc cggccgagcg ccaggtcaat
      841 ggcgtcacgg cgttcctgac cgtgcaggag ggctgcgaca agttctgcac cttctgcgtg
      901 gtgccctata cgcgcggcgg cgaatggtcg cggcccgtga acgacatcct cgaagaggcc
      961 cgccgcctgg ccgaccaggg tgtgcgcgag gtcaccctgc tgggccagaa cgtcaacgcc
     1021 tatgacggcg atggctccac cctggccaag ctggtgcgcc agctggccaa gatcgatggc
     1081 ctggaccgca tccgctatac gacgagccat ccgcgcgaca tgggcgatga cctgatcgag
     1141 gctcatggcg agctgccgga actgatgccc tatctgcacc tgccggtgca ggcgggatcg
     1201 gacaagattc tcaaagcgat gaaccgcgac cacacggccg agagctatgt gcggttgatc
     1261 gagaagatcc gcgctgcgcg gcccgacatc gccatgagcg gcgacttcat cgtcggcttc
     1321 cccggcgagc gcgacggcga tttcgaaaag accctggagc tggtccgcga ggttggcttc
     1381 gcctctgcct tctcgttcaa atattcgcgc cgccccggca cgcccgcctc ggccatgacc
     1441 ggccaggtcg acgaagcggt gaaggcgcag cggctggagc gcctgaacca gctgctggat
     1501 gagcagcaaa aggccttcaa cgcgacccag gtcggcaagg tgctgccggt gctgtttgag
     1561 aaaaagggcc gtcacgccgg tcaaatcgtc ggccgcagtc cctatctcca ggccgtccac
     1621 gccgagggcg gggatcatct gctcgggacg atcgttccgg tccgtatcga cagcgccgcc
     1681 aagatgagcc tgggcggcgt tctggaaacc tctgccgttc tggagaccgc ttgagccgca
     1741 cccctgagtt cctgccgctg tcggacgacg ccgtccatgc cgtctccggt cccggtggcc
     1801 gtcacgccgc cctgatcgag gacgccttca gggtgttgat cgagaccccg ggcggcggcg
     1861 tcaccatcac gggcgacgtc aagggccgca cgggcgccaa gcgggcgctc aacgatcttg
     1921 cagcgcgcgc cgacgccggc gaggaagtgg tcgaggccga tgtgcggatc gccgtcggcg
     1981 aggcccagag caccggcggc caggcctcgg cgcgcacggt gcgcaagggc tcagtctcgc
     2041 ccaagaccaa gggccaggcc cgctacatgg aagccatggg cagccatccg ctgtcgtttg
     2101 gcctgggtcc ggccggcacc ggtaagacct tcctggccgt ggcccacggg gccggcatgc
     2161 tgctgcgcgg cgaggtcgat cgcctgatcg tcacccgccc cgccgtcgag gccggcgaga
     2221 agctgggctt tctgcccggc gacctgaacg agaaggtcga cccctacatg gccccggtct
     2281 gggaagccct gaccgacatc atgggcgccg agcagctgcg tcggcgccgc gagaagctgg
     2341 aaatcgaagt cgccccgatc gccttcatgc gcggccgcac cctggcccat gcgttcgtca
     2401 tcgtcgacga ggcccagaac tgctcgcgcc tgcagatgaa gatggtcctg acccgcctgg
     2461 gcgaaggctc gcgcatggtg gtgacgggcg atccgaccca gatcgacctg ctcaatccgc
     2521 gcgattcggg cctggcccac gccgtgtcga tcctggaagg ggtcgagggg gtttcggtct
     2581 cgcgcttcac cgccgccgac gtggtgcgcc acccgctggt ggagcggatc gtcaaggcct
     2641 acgacgccga cgcggcccag agcacgcccc gctgatgacc ccccaccaat gaccccccac
     2701 caatgaccat gactgtcgac atcgagatcg aggacgaggc ctggctgaag gccgagcccg
     2761 aaaccgaagc cctggtctgg cgcgccgccc aggcggtgct ggacgcccat caggacatcg
     2821 agggccaggg gatcgtcatc ctgctggccg acgatgacag cgttcaggcc ctgaaccgcg
     2881 actttcgcca gaaggactat gccaccaacg tcctgtcgtt cccgtcgccc caaggacctg
     2941 aggccaatcc ggaaggtcag atcggcgata tcgtcctggc gttcggcgtc tgccagcgcg
     3001 aagcgatcga gcagggcaag cctcttgcgc atcacctgca acatctggtg gcgcatgggg
     3061 tattgcatct tctaggatac gaccacgaag gcgacgaaga ggcggagacc atggaggccc
     3121 tggaacgcga gatcctggcc accctggata tcccggaccc ttacgccgcc gatgtgaccg
     3181 agcaggggtg actccagagg ccatgccaag cgacgactct agtccacagc ccggcggtcc
     3241 agctcgacag agccggggcg tgcgggcgtt ttttcgcagg atgcgcaagc agctttcggg
     3301 ggcggcggaa cccgcccgcc cgcccgagcc tgtgcaggac ggcgctgtcg acatcgtcga
     3361 ccaggccgag gccttcaaga ccctgcgcgt cgccgacgtg atgacgccgc gcgccgacat
     3421 tgtctcggtc gagctgtcca cgccgttcga cgccctgatc gcccagttca ccgaggccga
     3481 acactcgcgg atgccgatct accgagaaac gctcgatgat ccggtgggcg tggtccacgt
     3541 caaggacgtg ttccgcatgc tggcggccgg cgagaagcgc cccagcggaa ccgacgtcgt
     3601 tctgcacaag gtgcggcgcg aggcgctgta cgtgcccgcc tcgatgcggg ccgccgacct
     3661 gctgctgcgc atgcggacca gccgcatcca cctggccctg gtcatcgacg aatttggcgg
     3721 caccgacggc ctggtcacca tggaagacct gatcgaggcg gtggtcggcg acatcgacga
     3781 cgagcacgat gacgccaccg tcgccggcat cgtcgcccgt cccggcgggg tgttcgaggc
     3841 tgacgcccgc gcgccgctcg aagaactgga agcggccctg ggtcgcgaac tggccccgcc
     3901 cgacatggaa gaagacatcg atacggtcgc tggccttgtc gtggccctgg ccggccgcgt
     3961 gccgcagcgg ggcgaggtga tcgcccatcc gcatggctat gacctggaag tggtcgaggc
     4021 cgatccgcgc cgggtgcgcc gggtgcgggt gcggccggcc gccatccccg gcgcggcctc
     4081 tcaaccggcg gaagctgcgg cctcgtgaac ctgaaactgg cgacgccgtg gcgcgaacgg
     4141 ggcctggccc tgttggcggg tctcgccgcc gccctggccc atccgccgtt cggcgtgctg
     4201 ccgggcctgc tgggctacgc cctgctgctt tggctgctcg acacgatcga tgacccgcgc
     4261 ccgctgcgcg cggccttttt ccggggctgg ctggcggggc tgggctattt tggtctgggc
     4321 acctggtgga tcggcgaggc ctttctggtc gacgcggcca accagggctg gatggccccg
     4381 ttcgcgatcg ccgccatggc ggggggtctg gccctgttct ggggcgtggc cgccgtggcg
     4441 tatcgcctgg ccagaccgcc gggcgtgcgc cgcgtgctgg tgttcgccgg cgcgtttgcg
     4501 gcgctggaat ggacccgagg ccatgtcctg accggctttc cctggaacct gcccggcgag
     4561 acctggaagg ctggctcggc cccctcccag gccgccgcca tcgtcggcgc ctatggcctg
     4621 acctggatca ccctggcgat cgccgccgcc ccggctgtct ggcgcgacgg cctggccgga
     4681 cgcaaggcca tcgcggcggc tgcggccctg ctggcggcgc tctacatcaa tggctggatg
     4741 cccgccgcgc cggcccccaa gaccaccacg cccaagacca ccacgcccac ggtccgcatc
     4801 gtccaggcca atatccagca ggacctcaag tgggacgagg gccgcttcgt ccagatcgcc
     4861 cagtcctatg tcgacctgac cgcccggccc tacaaggacg gcaagccggc cgacatcgtc
     4921 gtctggcccg aaggcgccct gccgctgtcg ctgaacgagt atctggcgcc cggcgtctgg
     4981 gttcgcgacg ccatcatcga cgccctgcaa cccggnnnnn nnnnncgcca ggacgggacc
     5041 ctgcaggatc cggtctattt caacagcctg gtcgccctga gacgcggcgc cgacgacatc
     5101 gaggtggtcg ggatctatga caagcaccgc ctggtgccgt tcggcgagtt cctgccgatg
     5161 gacgccctgc ttacggacct gggcgtcaag agcatggccc atctgggcga cggcttcgag
     5221 accggcccgc gcccggcccc cctgcaggtg gcgcccgatc tgatcgtcca gccgctgatc
     5281 tgctacgagg cgctgtttcc gggtctggcc aagcctgatc ccagggtccg cgccctggtc
     5341 aatgtctcga acgacgcctg gttcggtgtc acctccggac cactgcagca cctcaacctg
     5401 gccagctatc gcgccatcga gacgggcctg ccgatcattc gcgccacgcc taccggcgtc
     5461 tcggccctga tcgacgccag gggcaagatc gtgggcggcg cccaattgaa cctgggcgat
     5521 gcgggcgtga tcgacggggt tctccccgat atggggacgg ccacgccctt tggccggctg
     5581 ggaaacaccc cgtttatcct gatgattttg atatcggcgt tcgccggagt tcgcctgaag
     5641 gtgggtaagg gcttttaatt attacaattt cgttagagat aggctgaata aaaaccgcaa
     5701 tttcagctat caagcgtagg catgagcgct ccctccgacc aagaccgtca ccccaatccg
     5761 atcgacctgc atgtcggcgc ccgtatccgg atgcgtcgca agatcctcgg cgtcagccag
     5821 gaacggctgg ccgaggacct gggcctgacc ttccagcagg tgcagaaata cgagcgcggc
     5881 gccaatcgcg tcagcgccag caagctgtat gagatcgccc gcagcctgca gtcgtcggtc
     5941 gcctatttct tcgagggcct ggccgatccg acctccgccg agggcatggc cgagacgggc
     6001 tcggagcagt tcgtgcacga cttcctgatg acccccgaag gcctggagct ggccagcctg
     6061 ttccccaaga tcaatcgggg caaggtgcgc cgccggatcc tggatctggt ccgctcgatg
     6121 gccgacgaag agacgcccga agccgaagat tagggtgtga cggtcggcgc cgcttgccga
     6181 cataaacata tctttatatg gtcgccggct aactgggtgg tcacatcgtc cgcctgggcg
     6241 aatttattgg ccggagctgt cccttgagcc gttcgtccta catcttcacc agcgagagcg
     6301 tctccgaagg ccaccccgac aaggtcgccg accggatcag cgacaccgtg gtcgacgcct
     6361 tcctgagcgt tgaccccgaa gcccgtgtcg cctgcgagac cctggtcact accaaccgca
     6421 tcgtgctggc cggcgaagtc cgggccggaa agcccgatgg cgacaaggcc gccaacaagc
     6481 agctgaccaa ggacatcctc aagtcgctgg agcccaaggt gcgggccgcg atcaaggaca
     6541 tcggctacga gcagaagggc ttccactggg agaaggccaa gtttgccaac tacctgcacg
     6601 gccagtcggc gcacatcgcc cagggcgtgg acgccagcga caagaaggac gaaggcgccg
     6661 gcgaccaggg catcatgttc ggctacgcca gcaccgagac cccggagctg atgccggcca
     6721 ccctgcaata cagccacaac atcctcagcc acctggccag gctgcgtcac agcggcgagc
     6781 tgtcggcgct ggagccagac gccaagagcc aggtgaccct ggaatatgac gccaacggca
     6841 agccgacccg cgtcgtctcg atcgtcgtat cgcacc
//
LOCUS       DAOMEO010000021         6866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1579_length_6866_cov_2.694612, whole genome shotgun sequence.
ACCESSION   DAOMEO010000021 DAOMEO010000000
VERSION     DAOMEO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1579_length_6866_cov_2.694612"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLH31_00940"
     CDS             <1..336
                     /locus_tag="PLH31_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87848.1"
                     /translation="GCDLLLIEGKVLAQTDHDAGDILSAFAGAAGAAKIVVMWGGDAT
                     EGLQLQEAGADLVVQKPISASDLVDRLKTLFPSHDVGGQGADTDEASHPPPRRNVHPP
                     AIHAEVCAL"
     gene            293..787
                     /locus_tag="PLH31_00945"
     CDS             293..787
                     /locus_tag="PLH31_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR87849.1"
                     /translation="MSTRPPSMRKFVRCSLVAGAAHLDQRSCNPAETAMRVLFVDDNA
                     MNRTVVKSMLAAAEVEMVEAADAEIGLALVEAQHFDVILMDYRMPRMDGLAAIGAIRA
                     RPDSKARLPIIMVTADDGPDLRNQAIAAGADDLLHKPIQMQPLFDAIGQILARGDVGA
                     GMLV"
     gene            complement(1796..3175)
                     /gene="otsA"
                     /locus_tag="PLH31_00950"
                     /pseudo
     CDS             complement(1796..3175)
                     /gene="otsA"
                     /locus_tag="PLH31_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285819.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
     gene            complement(3172..3942)
                     /gene="otsB"
                     /locus_tag="PLH31_00955"
     CDS             complement(3172..3942)
                     /gene="otsB"
                     /locus_tag="PLH31_00955"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HQR87850.1"
                     /translation="MTAQILAFPDLLPPPPPLDLAACALFLDLDGVLAPIVARPTDVG
                     PDPRRNFILRLLSGRLDQRLAIVSGRTLAEVSTITENAVRAVAGVHGLERRTVLGHEV
                     IATADPDLHEAVAVFRALARAQPELLVEEKGLSVALHYRACPSACEAVREIARRLEAA
                     TGLVLQEGDMVAELRTAGADKGQAVGAFMAEWPFQGSRPIFVGDDLTDEDGFAAAAGA
                     GGYGILVGSGRQTMARYRLADVPAVLDWLEAAITEPVS"
     assembly_gap    4913..4922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4923..5164
                     /locus_tag="PLH31_00960"
     CDS             <4923..5164
                     /locus_tag="PLH31_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR87851.1"
                     /translation="SLLIKNHSAVELVARLVERDLVTRRDSDADRRRVVLDLRPRGAE
                     ILEGISRRNLGRLNQGADILENIIEAARRAASSRG"
     gene            5395..5754
                     /locus_tag="PLH31_00965"
     CDS             5395..5754
                     /locus_tag="PLH31_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87852.1"
                     /translation="MIDHDKETILLICGDETDAATIMTNLYEADIRVVGPIHTARMAL
                     AMAAQTSPTLALLAGQPTGERKAAELAQAMMSTWGVRSMLLNPTEHDAHEASDWRASE
                     HQVALVRRALTQTLPSI"
     gene            6034..>6866
                     /locus_tag="PLH31_00970"
     CDS             6034..>6866
                     /locus_tag="PLH31_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR87853.1"
                     /translation="MASSDAFWPALAVRARRPTRLAFIVTALSVAVSTAPFTETQAAG
                     ARHGLLILLILLLGWIVMTALDIAAALYLRRFRVDVEDNLLARKHLTQVRILRRASAV
                     IVGIFTISLALMTIPGVRQWGVSLLAAGGAASLILGLALQPLLTNLIAGIQIAITQPI
                     RIDDAVIVEKEWGNIEEINATYVVVRLWDWRRMVLPLSYFIQTPFQNWTRESAALIGT
                     VMLYVDHAAPIDALRAKLEEIAKASRLWDGNVVNLAVTDITAQVLEVRCLVSGRNAPT
                     V"
BASE COUNT         1246 a         2247 c         2189 g         1174 t
ORIGIN      
        1 ggctgcgacc tgctgctcat cgaaggcaag gtccttgccc agaccgacca tgacgcaggc
       61 gacatcctca gtgcatttgc cggcgctgca ggcgcggcca agatcgtcgt gatgtggggc
      121 ggcgacgcca cggagggact tcagcttcaa gaggccggcg ccgatctggt cgtgcaaaag
      181 ccgatctcgg ccagcgacct cgtcgaccgg ttgaagacgc tcttcccgtc tcatgatgtg
      241 ggtggccaag gcgcggatac ggatgaggcc tcgcatccgc ccccgcgacg aaatgtccac
      301 ccgcccgcca tccatgcgga agtttgtgcg ttgtagtctg gtggctggcg cggctcactt
      361 ggatcagcgc tcgtgcaacc ctgcggagac cgcgatgcga gttctatttg ttgatgacaa
      421 cgcgatgaac cggacggtcg tcaagagcat gctggccgcc gccgaggttg agatggtcga
      481 ggccgccgat gccgaaatcg gcctggccct ggtcgaggcc cagcacttcg acgtgatttt
      541 gatggactat cggatgccca gaatggacgg cctggcggcc ataggggcga tccgggctcg
      601 cccggactcc aaggcgcgct tgccaatcat catggtcacc gccgacgatg gccccgattt
      661 gagaaaccag gcgatagccg caggcgccga tgatctcttg cacaagccaa tccagatgca
      721 gccgctgttt gacgcgatcg gtcagatcct ggcgcgcggc gacgtcggtg cggggatgct
      781 cgtatagatt agccacaacc ggtagcgcaa aaagtgcggc ctcggcgtgt gccgctggag
      841 atacggttct tcgggtagcc tacgacgcgt cgacagcgaa acggcgctgc gtgcaaatcc
      901 agccgtgacc tgcgaacgaa ttgggccttc gcccgacggg gtagaacacg cggtatctct
      961 tcgcctcgtg gcgcggccag cttctggtga aggcggatac ctcaccacta cgccggccgt
     1021 tcttgggttg gtggtggtgg cgaacgaaac tctcggcatg ttcgccacgg cgagaatgat
     1081 tggaaaggct agagggttgc tgagcggctt tcccgcagtg cgcgaggcct cgcaatagtc
     1141 ccgcagccgg cgccaaaatg ctgtaaatct attatttttc aagattttga ccccacctaa
     1201 ccgcaccgcc caaaatttgg cggccgcgca ggacattgtc gcccctgcag gcaacgggcg
     1261 acatcgacga ggtttttgac aacctcgcgc tcagatgaaa aaaatagcat gagttccatt
     1321 cccggccttg gtggaaacgg gcgatctcgc ggcgcaaagc cgaaggcgcg acacgccttc
     1381 cagtccgacc acgcttctcg aacacaccct cgcaaccaac ggtgcgcggg atccgtctcc
     1441 atgcgcggat gccagagcaa ggataccgcg aactcgggaa tcggaatggg aaggctgaag
     1501 ctgaccagcc cctctcggag gccccgcata tggcgttttc gggagtttgg cagccgtacc
     1561 tatcgccctt cctacaggcg cgccgtgatt ttttcctgct cacggctgca tgaggcgacg
     1621 ctggttacgc aaggcgagcg ttacgatttt tgccgttttc tacaaggaag acgccgcgcg
     1681 tatccgccgt gagaacgagc agtttctggc gccctgccct gaccggccaa tgggtcagtc
     1741 gttctagcgg gcaggcagcg caggaatggc ctgagcgcta gactggagcc aggcactacg
     1801 aagcagcatc gccttccagc gccgagacga agcgatcacg ccaggcagcg acgtcgtcgc
     1861 ggacgacacc ctccatcagc ctggtccagc gcgcgatccg ctcgcgacgg ggcatggcca
     1921 gggccaactc gagcgcttcg gaggtctcct cccgactgaa cggattgacg atcagggcgt
     1981 cttccatctg ctcggccgcg ccggcatacc gcgacagcac caggaccccg gggtcctcgg
     2041 ggtcctgcgc cgcgacatat tccttggcca ccaggttcat cccgtcccgc aacggcgtga
     2101 ccaggcccac gtgcgccgcg cgatagacgc ccgccagctc gtcgcgtcgg tgggtgcggt
     2161 tcacgtagcg gatcggcacc cagtcgaccg tggcataggc gccgttgatg cgccccgaaa
     2221 cgccgtccag ccgtgcgcgg atttcctgat aggcttggac cgcatcgcgg ctaggcgtgg
     2281 cgatctgcag caggaacacg cgctccgccc agtccggatg atcagccagc atctgctcat
     2341 aggccaggaa gcgctattcc aggcccttgg agtagtccag gcgatcaacg ccgacgatca
     2401 tcttgcggcc tgtctggctg agcctgacac gatcgaacgt tgccttggcc gcctcgcctt
     2461 gcgcgagctc ggcgaaagcg gtggcctcga ttccaatcgg gaaggcgcca atgcgagtgc
     2521 gacggccgtt ggcttcgatg acgccatcgt cgatcaccag ttccagttcg cggccgacat
     2581 agtcgctgaa cgcctcgagg gcgtcgcgcg tctggaagcc gaccagatca taggccagca
     2641 gcgattccac cagttgccga tgctggggca aggtggtcag gagccgtcgc cccggccacg
     2701 ggatatgcag gaagaagcca atacggttgg tcacgccaag gcggcgcagc tcctcggcca
     2761 gcgggatcag gtgatagtca tggatccaga caaggtcgcc agggccgatc aagggcgcca
     2821 gcgtctcggc gaagcgccgg ttgacccgct gatagccttc gccaaacgag cgttcatagg
     2881 ccgccaggtc ggtgcgggag tggaacagcg gccaaagcgt acgattggcg tagccgtcgt
     2941 aatattcctc gcgatcctgc tcttccagat cgaccagggc cacggtcacc ccgccgaccc
     3001 tttgcatcga gatctggccg gtaaaggcgt cggtggtctc gccgctccag ccaaaccaga
     3061 cgccggaata ttcgcgcagg gcggcggcca gggccatggc aagccctccg gccgtgccga
     3121 cgccggcgtc gctgggcggg ttcacccggt tggagacgac gatgagccgg ctcatgacac
     3181 gggctcggtg atcgcggcct caagccagtc gaggacagcg ggcacatcgg ccagacgata
     3241 gcgcgccatg gtctgtcggc cagaaccgac cagaatccca tagccaccgg cccccgcggc
     3301 tgccgcgaag ccatcctcgt cggtcaggtc atcgccgacg aagatcggac gagagccctg
     3361 aaacggccat tccgccatga aggcgccgac agcctgaccc ttgtccgccc cggccgtgcg
     3421 cagttcggcg accatgtcgc cttcctgcag aaccagacca gtggcggcct caaggcggcg
     3481 ggcaatctcc cgaactgcct cgcaggctga agggcaggcc cgatagtgta gcgccacgct
     3541 caagcccttc tcctcgacaa gcagttcggg ctgagcgcgg gccagagccc ggaagaccgc
     3601 cacggcctcg tgcagatccg gatcagcggt ggcgatcact tcgtgaccca acaccgtacg
     3661 ccgttccagg ccatggacgc ccgcgacggc gcgtacggcg ttctcggtga tggtcgacac
     3721 ctcggcgagg gttcgaccgc tgacgatcgc cagccgctgg tcaaggcgcc cggagagcag
     3781 gcgcaggatg aagttgcgtc gaggatctgg gcccacatca gtgggccggg caacgatcgg
     3841 cgccagtacg ccgtcgaggt cgaggaatag cgcgcaggcc gccagatcaa gcggcggggg
     3901 aggaggcagc aagtctggaa aggcgagaat ctgggccgtc atagcggact ccatggctgc
     3961 gcccgaaaaa ctttcggacg cgagacgtcg atacgccgac caagaatcgg tgacggcgtc
     4021 agaggcgacg ggatcagccg ccgcggcgaa tggagcgtgt cagagccggc gcggccgtgt
     4081 ggttcggcct agcggcgtgg cgcggcgggc gcgcggaagg cgaaactcgc cctggcggat
     4141 cggtggtctc atctgtggcc ccttgcccaa ttcggcgccg tgcgcggcgc ggtctttctc
     4201 cggattgatg gatcggccgt cgaagggacg gcgaacctgt cgcacgatgc gacgtgagcc
     4261 aaagctctgt ccaaggcggt gcgccccgag tggatgaccc tagcaggctg aaggcgagca
     4321 agaccgcgcc tcacgcgata gtgacgccgg tttgaccgcc gccctgtccc ggcaggacgt
     4381 gaagataggg cccgcgaaca ggccgcatct ccagatcgtc caactgcaat tgggatccag
     4441 cgaaggcgcc tgccgccacc attacggcgg cgccgaccaa aactgtcagg atctgttttc
     4501 gccgtcgcaa cgccgacatg cagagacttc tgaaggcccg cgccggcgga gcgtcagcct
     4561 cgaggcggca aaccttccaa accaacatga cggataaata tcgtgagacg atctattcgt
     4621 caaatgccgc cgacttcagc cctgcttgcg actcggcctt cgtgttaggc ttgtggttgg
     4681 tcgcgagcat ctcgcgcgtc cttttcggat ctcatcgtga cgcctgtctc tccgccctca
     4741 caggatccga ccgatgccgg cgccatcacc caggatgact accgcgcgct cggcgaattt
     4801 cgccgcgcca tccgccagtt cctggccttc agcgagcagg gcgcgcgcga ccacggcctg
     4861 acggcccagc agcatcaggc cctcctggcc atcaaggcgc atgccgggcc ggnnnnnnnn
     4921 nnagagcctg ctgatcaaga accatagcgc cgtcgaactg gtcgcccgcc tggtcgaacg
     4981 ggacctggtg acgcgccgcg attcagacgc ggatcggcgc cgggtggtgc tggacctgcg
     5041 cccccggggg gccgagatcc tcgaaggcat ttcccgacgc aatctggggc gactgaatca
     5101 gggtgcggac attctggaaa atatcatcga ggccgcgcgc cgcgcagcct cttcgcgggg
     5161 ctagcaaacc aaggctggcg tccgggcgct ccacaatcgg ccggtgtaag ggcgccccgt
     5221 cgacttgccc tccgagcacc tgccggccgg caggcagtca tcccgatcat ctccccttct
     5281 gaactcagcc agcgaagcct cacaacttcg tcaggttgat gtgtcctgag gccaagttag
     5341 atcgtgatac gatctttgtg ggtcggcgta acgaccctga aaagtgggga cgcaatgatt
     5401 gatcatgaca aagagacgat cctgctcatc tgcggtgatg aaacagacgc cgccacgatc
     5461 atgactaacc tttacgaggc tgatattcgg gtggtcggtc ccatccatac cgccagaatg
     5521 gccctggcga tggccgccca gaccagcccg acgctggcct tgctggccgg ccagcccacc
     5581 ggagagcgca aggctgccga actagcccaa gccatgatgt ctacctgggg cgtgcgctcg
     5641 atgctgctca accccaccga acatgacgcc cacgaggcgt ccgactggcg cgcgtccgaa
     5701 caccaggtcg ccctggtgcg ccgcgccctg actcagaccc tcccctcgat ctgacgtgag
     5761 gggccctgcc ccgcccctgc ctgctgtagc ggctgcggta atcgacgagg attgtacgcc
     5821 ggcacatcca ccttcctggc gggcgccccg tcgggagccc cgccccgtga caggcgttga
     5881 actgtgatgt ctttatctct cgttcggctt gccggcctgc catgggttcc cgcttgggcg
     5941 ccgccatggg ccatcagctt ggcgctcatt gtgctcgcga tcgtcgtggc gatcgccgca
     6001 catcaggcgg cctccctcat cgtacgccgt ggcttggcta gcagtgacgc cttctggccc
     6061 gcgctcgcgg tgcgggccag aaggccgaca cggttggcgt tcatcgtcac cgccctgagc
     6121 gtagcggtct ccaccgcgcc ctttacggag acccaggccg ccggcgcccg gcatggactt
     6181 ctgatcctgc taatcctgct gctgggctgg atcgtgatga cggcgctgga catcgccgcc
     6241 gccctctacc tgcggcgctt ccgggtcgat gtcgaagaca accttctggc ccgcaagcat
     6301 ctgacgcagg tacggatcct tcgccgggcc agcgccgtca tcgttggcat cttcacaatc
     6361 tccctggccc tgatgacgat cccgggcgtg cgccagtggg gcgtgagcct tctggcggcg
     6421 ggcggggccg ccagtctgat cctcggcctc gccttgcagc ctctgctcac caatctgatc
     6481 gccggcatcc agatcgccat cacccagccc atccgcatcg acgacgccgt catcgtcgaa
     6541 aaggaatggg gcaatatcga agagatcaac gctacctatg tggtggtgcg cctgtgggac
     6601 tggcggcgga tggtgctgcc cctgagctac ttcatccaga ccccgttcca gaactggacg
     6661 cgggagtcag cggcgctgat cggtacggtg atgctctatg tcgaccacgc cgcgccgatc
     6721 gatgccctgc gcgcaaagct ggaggagatc gccaaggcct caaggctatg ggatggcaac
     6781 gtggtcaatc tggccgttac cgacatcacg gcccaggtgc tggaagtgcg ctgcctggtc
     6841 agcggccgca acgcgccgac ggtctt
//
LOCUS       DAOMEO010000022         6744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1607_length_6744_cov_2.367768, whole genome shotgun sequence.
ACCESSION   DAOMEO010000022 DAOMEO010000000
VERSION     DAOMEO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_6744_cov_2.367768"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..671)
                     /locus_tag="PLH31_00975"
     CDS             complement(69..671)
                     /locus_tag="PLH31_00975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR87854.1"
                     /translation="MIYLCRHGQTEWNRERRLQGQTEADLTPLGRLQAAAMADLLLDL
                     TAREPSADWRIVASPLRRARDTAQAIADRLGRKIEFDDRLMELTVGEWEGRLYADVQR
                     ENPEAFDSPEWFFTAPGGETYEQVMARVSDWLAEQAAEPGRRLIVVSHGIAGRLLRGA
                     YAGLSREETLAQDVPQDALYRLHCGQIDRLDCEPVEEPAC"
     gene            complement(708..1847)
                     /locus_tag="PLH31_00980"
     CDS             complement(708..1847)
                     /locus_tag="PLH31_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HQR87855.1"
                     /translation="MDFALSDDQIAIQDAARAFAEGQLAPHSAEWDEKKHFPVDVLRQ
                     AAELGFAGIYVQEDVGGTGLSRLDASIIFEALSYGDVPTAAYLTIHNMAAWMIDRFGS
                     EDLRRRYLPRLTTMELIASYCLTEPGSGSDAANMRTSARLDGDHYVLNGGKAFISGGG
                     VSDVYVVMARTGGDGAKGVSAFVVEKGTPGLTFGANERKMGWNSQPTAQVNFDDCRVP
                     VANRIGQEGEGFRFAMMGLDGGRLNIASCSLGGAQLALDTAKAYLETRNQFGKPLKDF
                     QALQFKLADMATELEAARLMVRRAAHALDSKDPQATKLCAMAKRFATDAGFQVANDAL
                     QLHGGYGYLQDYPLERIVRDLRVHQILEGTNEIMRVIIAREMFRQ"
     gene            2106..2549
                     /locus_tag="PLH31_00985"
     CDS             2106..2549
                     /locus_tag="PLH31_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HQR87856.1"
                     /translation="MRKLKLFFVALAALVGFASAPAHAGGEGHGVWRNPKDNVRVEIK
                     SCGASTCGVVVYASPKAQADARKSGYDTLIGQQLLREFEPIGNGAMRGKVFVPTLKVT
                     LSGTAEFIDARTMRAKGCVLGKVLCKSQIWTRVDTVTAGTSRVSS"
     gene            2742..4244
                     /locus_tag="PLH31_00990"
                     /pseudo
     CDS             2742..4244
                     /locus_tag="PLH31_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            4397..4855
                     /locus_tag="PLH31_00995"
     CDS             4397..4855
                     /locus_tag="PLH31_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HQR87857.1"
                     /translation="MRIVKLAVIAVSATLGMASVSAHAASAADTARTYGVWRNPKDSV
                     HLEIKDCGETACGVVIWASDRAKADARKGGSPNLVGLRLLRDFAPDKNGAWRGKVFVP
                     DLNMTFSGAAEVLDTNTLRAKGCVLGGIICKSQIWTRVDAASIIASTATR"
     gene            4917..>5422
                     /locus_tag="PLH31_01000"
     CDS             4917..>5422
                     /locus_tag="PLH31_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQR87858.1"
                     /translation="MTTPPLAPYPATRLRRVRQADWTRRLVRETEVRPSDLIWSMVVH
                     EGEGVIPVASMPGVERLSVKEAAKAAIRARDLGIPAIAIFPHIDGARKDATGSIAADP
                     DGVIPRAVKAMKDAAPEVGIMCDVALDPFTDHGHDGVVEGGRILNDPTIERLIEQGLM
                     QAQAGADIL"
     assembly_gap    5423..5432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5433..5931
                     /locus_tag="PLH31_01005"
     CDS             <5433..5931
                     /locus_tag="PLH31_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQR87859.1"
                     /translation="LAPSDMMDGRIGALRAALEGANFQDTMIMSYAAKYASAFYGPYR
                     DAIGSAKLSAGQGDKKTYQMDPANTEEALREVALDIAEGADMVMVKPGMPYLDILRRL
                     VDEFRMPTYAFQVSGEYAMIKAAGLNGWIDEERAILESLTAFKRAGAAGIITYFAPWA
                     AEKLG"
     gene            5931..>6744
                     /locus_tag="PLH31_01010"
     CDS             5931..>6744
                     /locus_tag="PLH31_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR87860.1"
                     /translation="MTHPGSAFRPGRFYGAVAVEHHLADARVAHLAATTPPEEVAEHS
                     HDDAHFVLATRGRYVTTAHGDEAEGPVLVFNPPGVVHRDRFEGVDGWFMAVSVEADAW
                     RDLTSQSGAAVDALRLRSPRAVGAALRLTRAALSRDVEQIDLETHALALAAEAMAPMQ
                     RESQKRPAWLDTAETYLADGFDQPISIADIARAAGVHRVHLARGYQRWIGAAPGERLR
                     ARRLERAADLLMHGRMGISEIALQAGFCDQSHLNRQFRAAYGLTPGEFARLCG"
BASE COUNT         1159 a         2310 c         2123 g         1142 t
ORIGIN      
        1 cttgggcctg ttgagcgtga tccggccgac gtttttatga acgcggacaa tgatttcggc
       61 cgtatcgttc agcatgccgg ttcctcgacg ggttcgcagt ccaggcgatc aatctgaccg
      121 caatgcaatc gatagagcgc gtcctggggc acgtcctgag ccagggtctc ttcgcgcgac
      181 agaccagcat aggcgccgcg cagcagccgg ccggcgatgc catggctgac cacgatcagc
      241 cggcgcccgg gctcggcggc ctgctcggcc agccagtcgg agacacgcgc cataacctgc
      301 tcgtaggtct cgccgcccgg cgcggtgaag aaccattcgg gactgtcgaa ggcctcggga
      361 ttttcgcgct gcacatcggc atagagacgc ccctcccatt cgccgaccgt cagctccatg
      421 agtcgatcgt cgaactcgat cttccggcca agccggtcag cgatggcctg agccgtatcg
      481 cgcgcccgac gcagcgggct ggcgacgatc cgccagtcgg ccgaaggttc gcgcgcggtc
      541 agatcaagca gcaggtcggc catggccgcg gcctgcaagc gccccagagg cgtcaggtcg
      601 gcttcggtct gcccctgcag gcggcgttcg cggttccact cggtctggcc gtggcggcag
      661 agatagatca cgtctggtcc tgggttcggc ggaggctggc cgtggctcta ctgccgaaac
      721 atctcgcgcg ctatgatcac gcgcatgatc tcgttggtgc cttccaggat ctggtgcacc
      781 cgcaggtcgc ggacgatgcg ctccagcggg tagtcctgca gatagccgta ccctccatgc
      841 aactgaaggg cgtcattggc cacctggaag ccggcgtcgg tggcgaagcg tttggccatg
      901 gcgcacagct tggtggcctg cgggtccttg ctgtccagcg cgtgggcggc gcggcgcacc
      961 atgaggcggg cggcctccag ctcggtggcc atgtcggcca gcttgaactg cagcgcctga
     1021 aagtccttca gcggcttgcc gaactggtta cgggtctcga gataggcctt ggcggtatcg
     1081 agggcgagct gcgccccgcc cagtgagcac gaggcgatgt tcaggcggcc gccatccagc
     1141 cccatcatgg cgaagcgaaa gccctccccc tcctggccga tgcggttggc gacgggcaca
     1201 cggcaatcgt cgaaattgac ctgggcggtc ggctggctgt tccagcccat cttgcgctca
     1261 ttggccccga aggtgaggcc gggcgtcccc ttctcgacga cgaaggccga gacgcccttg
     1321 gccccgtcgc ctccggtgcg ggccatcacc acatagacgt ccgaaacacc gccgcccgag
     1381 atgaaggcct tgccgccgtt caggacgtag tgatcgccgt ccagccgggc tgaagtgcgc
     1441 atattggccg cgtctgaacc tgagcccggc tcggtcagac aatagctggc gatcagctcc
     1501 atggtggtca ggcgcggcag atagcgccgg cgcagatcct cggaaccaaa gcggtcgatc
     1561 atccacgcag ccatgttgtg aatggtcaaa tatgcggcag tcggcacatc gccgtaggac
     1621 agggcctcga aaatgatcga ggcgtccagg cggctcagac ctgtgccgcc gacgtcttcc
     1681 tgcacataga ttccagcgaa gccgagctcg gcggcctggc ggagcacgtc gaccgggaag
     1741 tgcttcttct cgtcccattc cgccgaatgc ggggcaagct gtccttcggc gaaggcgcgc
     1801 gcggcgtcct ggatggcgat ctgatcgtcg ctgagcgcga aatccatgcg cgaaatcctc
     1861 ccgtgaaggg cagaaccgcc cttgcctctc gttgctgcag gacgtgccgt gcggctacca
     1921 ggatgtcaac ggcgccgcgc ctgactttgc ccaatcgtcg cgctcggcgc ccgcgaccac
     1981 cgtgagcggc cctgtaacct gaaatccact gttacacctt gtgtagcgcc ttgaatgcca
     2041 cacccccttg ataaccatcc gctttgacct cagatcacgc tccctggagg cgtcactgtt
     2101 tcgtcatgcg taagctcaag cttttctttg tcgcgctcgc cgcgctcgtt ggtttcgcat
     2161 ccgctcccgc ccatgccggc ggcgagggtc atggcgtgtg gcgcaacccc aaggacaacg
     2221 tccgggtcga gatcaagagc tgcggcgcca gcacctgtgg cgtggttgtc tatgcaagcc
     2281 cgaaggccca ggccgacgct cgcaagagcg gctatgacac cctgatcggt caacagttgc
     2341 tgcgcgagtt cgagcccatc ggcaacggcg cgatgcgcgg caaggttttt gtgccgaccc
     2401 tgaaggtcac cctgtcgggc accgccgaat tcattgacgc ccgcaccatg cgcgcgaagg
     2461 gctgcgtctt gggtaaggtg ctgtgcaagt cgcagatctg gacccgtgtc gacaccgtca
     2521 ccgccggaac cagccgggtc tccagctaag cttctgcgcc ggctgaaggt ccggcggcga
     2581 caaggccctc gcgcgcctgt cgaaaacaag tcgcttgatc cctatcaaac ctcgcggcag
     2641 cgtgtaggtt tacagtgttt agttaggcgc ccgcagaggc gtcgactttg gcgaggacac
     2701 gcggcgggtc tctgacggtc ctccgctcag ggaagacgcg catgacggac acctccatgc
     2761 tcgaaagagc gccgcaacag caatctatcg aacatttcga cgtcatcatc gtcggcgctg
     2821 gcatatccgg agtcggcgga gcctatcatc tgacccagca acgcccgggc gcgcggttcg
     2881 tcgtactcga agccctgggc gatttcgggg gaacctggcg cagccatacc tatcccggca
     2941 tccgctccga cagcgacctc tatactttcg gctaccgctt caagccgtgg gtcgggccgc
     3001 ccatcgccag cgcggccgaa atcctcaaat acatgggcga ggtgatcgag gacaatgatc
     3061 tggcccgcca catccgctat ggccacaaga tcacccacgc cagctggtcc agtcaggaca
     3121 agctgtggac cctggacgtc acccgcagcg acggcgcggt ccagcgcttc accaccaatt
     3181 tcctgtggat gtgccagggt tactaccgac agcagcaggg ctacacaccg caatggcctg
     3241 gcatgagcga ttataagggc cagatcatcc acccgcagta atggcccgaa aaccttgact
     3301 acaagggcaa gaaggtcgtg gtcatcggct caggagccac ggcggccacc ctggtgccgg
     3361 ccatcgccgg cgactgcggt cacgtcaccc tgctgcagcg ttcgcccacc tattttgtgc
     3421 ccggccgcaa tgagaacgac ctagccaaca ccctgcgcga actgcagatc gacgaaacct
     3481 ggatccacga gatcgttcgt cgcaaggccc tgttcgacca ggagatgttc acccatcgcg
     3541 ccgtcgcgga gcccgaggtg gtcaaggccg aactgctgga gggtgtgaag gccttcctgg
     3601 gcgaggactt cgatatcgcc aagcatttca cgccgcgcta tcgcccctgg cgtcagcgga
     3661 tcgctttcgt gcccgacggc gacctgttcc agggcatcgc ctcgggcaag gcctcggtag
     3721 tgaccgatga gatcgagcgg ttcaccccga ccggtctgct gctgaaatcg ggtgagaccc
     3781 tcgacgccga catcatcatc acagccacgg gcttcaacct gaacgtcctg ggcgacatcg
     3841 acttcgtcgt cgacggcaag ccgctgaact tcgccgacac cgtcacctat cgcggcatga
     3901 tgttcaccgg cgtgcccaac atggtctggg tcttcggcta tttccgcgcc agctggaccc
     3961 tgcgggccga tctgatcgcc gacttcgtct gccggctgct ggcccacatg gacgagaagg
     4021 gcgtcagaca ggtcgaagtc gccctgcgtc cagaggacag ggatatgccg ctgtccggct
     4081 ggatcgatcc cgaaaacttc aatccgggct acctgatgcg cggcatgcat ctgttgccca
     4141 aggccggcga caagcccgaa tggcgtcaca cccaggacta ctggctggag aaagacgagc
     4201 ttcccgccat tgatcttgat gaggccagct tcgtctattc ctgaggccca ccggagtttg
     4261 agcggtcgga ttttcgcgat ccggccgctc cagcgaatgc cccgtgacat gtcaggagat
     4321 cgcgtcagac gcgcggcctc ttgctaatct tccgggtcgc ccccaattct gcgggtccct
     4381 ggaggcgccg attttgatgc gtatcgtgaa actcgccgtg atcgcggtct cggcgaccct
     4441 tggcatggcc tcggtttccg ctcacgcggc cagcgcggcc gacaccgcgc gcacctatgg
     4501 tgtttggcgc aatccaaagg acagcgtcca tcttgaaatc aaggactgcg gcgaaaccgc
     4561 ttgcggcgtc gttatctggg ccagcgaccg cgccaaggcc gacgcccgca aaggcggctc
     4621 gcccaacctg gtcggcctga ggcttctgag ggacttcgcc cccgacaaga acggcgcctg
     4681 gcgcggcaag gtctttgtgc cggacctgaa catgaccttc tcgggcgccg ccgaggtgct
     4741 ggacaccaat accctgcgcg ccaagggctg tgtgctgggc ggcattatct gcaagtcgca
     4801 gatctggacg cgcgtcgatg cagcttcgat catcgcgtca acggcgactc gctaggacgc
     4861 tcacttaagg gcgggggcgc cttgcctcgc ggccccgacc gcgcgaaacc ctagagatga
     4921 ccacgcctcc gctcgccccc taccccgcca cccgtctgcg ccgcgttcgc caggccgact
     4981 ggacccgccg cctcgtccgt gagaccgaag tgcgcccgtc cgacctcatc tggtcgatgg
     5041 tggtgcacga aggcgaaggc gtgatccccg tagcctccat gcccggcgtc gaacgcctga
     5101 gcgtcaagga agcggccaag gctgccatac gcgcccgcga cctgggcatc cccgccatcg
     5161 cgatcttccc gcacatcgac ggggcccgga aggacgcgac cggctcgatc gccgccgatc
     5221 ccgacggcgt catcccgcgc gccgtcaagg ccatgaagga cgccgccccc gaggtcggga
     5281 tcatgtgcga cgtggcgctg gaccccttta ccgaccacgg ccacgacggc gtggtcgagg
     5341 gcggcaggat tctcaacgat ccgaccatcg agcgcctgat cgagcagggc ctgatgcagg
     5401 cgcaagccgg cgccgatatc ctnnnnnnnn nncctggccc cgtccgacat gatggacggc
     5461 cgcatcggcg ccctgcgcgc ggccctggaa ggcgccaatt tccaggacac gatgatcatg
     5521 tcctacgccg ccaagtacgc ctcagccttc tatggcccgt accgcgacgc catcggctcg
     5581 gccaagctct ccgccggcca gggcgacaag aagacctacc agatggatcc ggccaacacc
     5641 gaggaagccc tccgcgaggt cgcgctcgac atcgctgaag gggccgacat ggtcatggtc
     5701 aagccgggca tgccgtatct ggatatcctg cgccgcctgg tcgacgagtt ccgcatgccc
     5761 acctacgcct tccaggtgtc gggcgagtac gcgatgatca aggccgccgg cctgaacggc
     5821 tggatcgacg aggaacgcgc catcctcgaa agcctgaccg ccttcaagcg cgccggcgcg
     5881 gccgggatca tcacctattt cgcgccatgg gcggctgaga agctgggtta gtgacccacc
     5941 ccggaagcgc cttccgcccg ggccggttct acggcgcggt cgcggtggag caccatctgg
     6001 ccgacgcccg cgtcgcccat ctggccgcca caacgccgcc cgaagaggtg gccgagcaca
     6061 gtcacgacga cgcccatttc gtgctggcca cgcgcggccg ctatgtcacc accgcccacg
     6121 gcgacgaggc ggaaggcccg gtgctggtct tcaacccacc cggcgtcgtc catcgcgacc
     6181 gcttcgaggg cgtggacggc tggttcatgg cggtcagcgt tgaagctgac gcgtggcgcg
     6241 acctgaccag ccagtcgggg gcggctgtcg acgccctgcg cctgcgatcg ccccgggcgg
     6301 tcggcgccgc cctgcgcctg acccgcgcag ccctgtcccg ggatgtcgaa cagatcgatc
     6361 tggaaaccca cgccctggcg ttggccgccg aggccatggc cccgatgcag cgcgaaagcc
     6421 agaagcgtcc cgcctggctg gacaccgccg agacctatct ggctgacggc ttcgaccagc
     6481 cgatcagcat cgccgatatc gcccgcgccg ccggcgtcca ccgcgtgcat ctggcgcggg
     6541 gctatcagcg ctggatcggc gccgccccgg gcgagcggct gcgggcccgc cggctggagc
     6601 gggccgccga cctgctgatg cacggccgca tgggcatcag cgagatcgcc cttcaggccg
     6661 gcttctgcga ccagagccac ctgaaccgcc agttccgtgc cgcctatggc ctgacgcccg
     6721 gcgagttcgc gcggctgtgc ggcc
//
LOCUS       DAOMEO010000023         6549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1648_length_6549_cov_2.821374, whole genome shotgun sequence.
ACCESSION   DAOMEO010000023 DAOMEO010000000
VERSION     DAOMEO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_6549_cov_2.821374"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLH31_01015"
     CDS             complement(<1..387)
                     /locus_tag="PLH31_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR87861.1"
                     /translation="MVLTASASALAMMVAMPAFAQQDTVDEVVVTGIRASLQKAMQQK
                     RNSDLVSEVVTAEDIGKFPDKNVADSLSRVSGVNVVTGSAAAGGFGENERVSVRGTDP
                     NLNLTLLNGHNMATGDWFVLDQTSGGR"
     gene            660..1379
                     /locus_tag="PLH31_01020"
     CDS             660..1379
                     /locus_tag="PLH31_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR87862.1"
                     /translation="MMFSERIGRLDAGGAQHAPLYKQLQRALRDAIHKKILAPDDALP
                     SISRITVRKALDGLVSEGLLTRRQGAGTFVAARVEKNFSKLSSFTEDMISRGRVPRSE
                     WISKSEGSVTPEESLTLGLSPGTPVYRFARIRYADGAPMAVEYTTVAAFALPSAETVG
                     TSLYEALEATGHRPVRALQRLRAILFTSEHADLLGVPVKDAGLLIERRGFLKDGRAVE
                     VTQSYYRGDAYDFVAELNALS"
     gene            1393..2472
                     /locus_tag="PLH31_01025"
     CDS             1393..2472
                     /locus_tag="PLH31_01025"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQR87863.1"
                     /translation="MEATTLTRPQAPAKSARLTPESTRMFLEAGQASQVVATQLGANA
                     ARVEAIAQRLRANPPRAVVTCARGSSDHAATFAKYLIETRTGVLTSSAGLSVSSVYAA
                     TQNMDDVLYLAISQSGKSPDLLASVRAAKEAGAFTIALVNDTSSPLAALVDEVLPLHA
                     GPELSVAATKSYIAALSAITQLVAAWTGDEDLAEGLAALPAGLDEAWTLDWTPAIERL
                     KLARNLYVLGRGVGFGVAQEAALKFKETCGLHAEAFSAAEVLHGPMALVKAGFPVLVF
                     AQNDESRRSVDEMAASLTARDADVLLVGAGEVGAGVLPALAAHPVIEPILMIQSFYRM
                     ANALSIARGYDPDSPPHLNKVTETV"
     gene            2472..3611
                     /gene="nagA"
                     /locus_tag="PLH31_01030"
     CDS             2472..3611
                     /gene="nagA"
                     /locus_tag="PLH31_01030"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQR87864.1"
                     /translation="MQAVLVNGRILAESGFVDGKSLVVENGRIAAILNAADAPADATR
                     HDLDGGLLVPGFIDTQVNGGGGVLFNDAPTVETIAAIGAAHRPFGTTGFLPTLISDDL
                     TVVDTAMRAVEAAIEAGVPGVLGIHIEGPFLNAKRKGIHDAGKFRVIDDAAAALLGSL
                     KIGKTLVTLAPETTTPDTIRKLTNNGVIVAAGHTNAAYGTMRKALAAGLTGFTHLFNA
                     MSPLTSREPGAVGAALESQTAWCGLIVDGRHVDPVVLRIALRTRPLDRFMLVTDAMPT
                     VGMIDKSFDLQGRQIHVVNGVCVDAHGTLAGSDLDMAAAVRNAISMLNLTVEDAVSLA
                     SASPAAFLGLAGQRGRIAVGQAADLVLLDDNLKVRETWIDGASSR"
     gene            complement(3690..>4160)
                     /locus_tag="PLH31_01035"
     CDS             complement(3690..>4160)
                     /locus_tag="PLH31_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87865.1"
                     /translation="LPGLWSNFGGIANVTLVDSDVEYRGIRGAATNPLGTFPTLAPAV
                     TLTERLSNLSRRAFNATLYYEADKFSARASAAYRSGFIDGTSGTGNVFEGYNSSINVD
                     ASMRYRLNDNLEFSLEGVNLTDEYRDRFTDLEADRNYEYNHFGRTIMFGVRYKM"
     assembly_gap    4161..4170
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4171..6396)
                     /locus_tag="PLH31_01040"
     CDS             complement(<4171..6396)
                     /locus_tag="PLH31_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87866.1"
                     /translation="MAGAASAQTAPAAGEEAVVEEVVVTGFRRSLDQALNVKRQSVAA
                     VDVIVAEDIAKFPDQNLAESLQRIPGISIVRDGGEGRAITVRGLGSQFTRVRVNGMEA
                     IATTSDGASANRERGFDFNVFASELFNSLVVHKTAEASLDEGSLGAVVDLNTGNPIAG
                     KDGLTLALSAQGQYNDLSKNLGPRLAGLIGYRAPSGVWAASMSAAYSKQEQIESTNNT
                     VRWAQARFNSVAGTNCFTTQNAGGTYVPSASCNQVALAFHPRIPRYGEVTHDRERLGL
                     TGSFQYQPTDRTKLSVDGLYSRYNAIRNEKWAEVLFRSNERSISVRDYTIDNNNNLIK
                     GTFDNAWVRIEHYERELDTTFYQFSANLTHEFNDQFKIQGLAGFSKSDADIPYETTII
                     FDDRDATGYKYDYTDMKKPVLAFGTSVTEPTNFQFAEFRDRPSNNTNEFKTVALNGDY
                     AITNELTWRAGAVYREFEFSLREGARDSAYCRAFTCAAGAYGAPVTAALTEQFELTGA
                     DAPAGTTTSWVVTNLQAAVSMIDLYNRPLIQQAGNTRGVTEKDTGGFVQFDVKSRFGD
                     IVDYALNLGVRYMKTEQSSTGISNGQTVTIERQYEDWLPAGNLALYPREDLILRGSIA
                     KVMTRPNLGNLTPGGSVDGFNYRVTSGNPQLDPFRATAYDVSAEWYFARESIFSVAVF
                     MKDIESFPVGVSSTGTYASTGLPLSLILASSPAAANPEGQPWTINTTTNGQGGKLKGV
                     EF"
BASE COUNT         1155 a         2100 c         2098 g         1186 t
ORIGIN      
        1 acgaccgccg ctggtctgat ccagaacgaa ccagtcgccg gtagccatgt tatggccgtt
       61 cagcagggtc aggttgaggt tgggatcggt gccgcgaaca ctgacgcgct cgttttcgcc
      121 aaagccgccc gccgcggccg agccggtgac gacgttgaca cccgaaacgc gcgacagaga
      181 gtcggcgacg ttcttgtccg ggaacttgcc gatgtcttca gcggtgacca cttccgacac
      241 gaggtcggag ttacgcttct gctgcatcgc cttctgcagc gaggcgcgaa tgccggtgac
      301 gacaacttcg tcgaccgtat cctgctgcgc gaaggcgggc attgcgacca tcatcgccag
      361 agcactggcg gacgcggtca ggaccatttt gtgaaagctt ctcataactc ggcttctccc
      421 catttcgccg ggcctgggcc cggagccgaa ccccaagccc aatctggtac caattatctg
      481 cggacgtccg cagattgcgg tacttctcag gcctcggaag cagactgtga cggaaattta
      541 tcagatgaca aggccaaaaa gaaaccaatt gcagaccatg ttactattgg tatgaaaatg
      601 gtcatatgct atcgagacaa catggtggcg ccgtcgattt gcgcggggag gcttgcttga
      661 tgatgttttc ggaacggatt ggccggctcg acgcgggcgg cgcccaacac gcgcccctct
      721 acaagcaact gcagcgcgcc ctgcgtgatg cgattcacaa aaagatcctg gcgcctgacg
      781 acgccctgcc cagcatttcg cgcatcacgg tgcgaaaggc gctggacggc ctggtcagcg
      841 agggcctgct gacccgccgc cagggcgcgg gcacctttgt cgccgcgcgg gtggaaaaga
      901 acttctccaa gctctcgtcc ttcaccgaag acatgatctc gcgtggccgg gtgccgcgca
      961 gcgaatggat cagcaagagc gagggctcgg tcacgccgga ggaatcgctg acgcttggcc
     1021 tgtcgcccgg cacgccggtc tatcgcttcg cgcgtatccg ctatgccgac ggcgcgccca
     1081 tggcggtcga gtacaccacc gtcgccgcct tcgccctgcc ctcggccgaa accgtcggga
     1141 cctcgcttta cgaagcgctg gaggccacgg gccaccggcc ggtccgcgcc ctgcagcgcc
     1201 tgcgcgccat cctgttcacc agcgaacatg ccgacctgct gggcgtgccg gtcaaggacg
     1261 ccggcctgct gatcgagcga cgcggcttcc tgaaggacgg ccgggctgtg gaagtcacac
     1321 aatcctatta tcgcggtgac gcctacgact tcgtcgctga actgaacgcc ctgtcctgaa
     1381 ggaagacgta tcttggaggc gacgaccttg acccgaccgc aggcccccgc gaaatcggcg
     1441 cggctgacgc cggaatcgac ccgcatgttc ctggaagccg gccaagcctc ccaggtcgtc
     1501 gccacgcagc tgggcgccaa cgccgcccgg gtcgaggcta tcgcccagcg cctgcgcgcc
     1561 aatccgcctc gcgccgtggt cacctgcgcg cgaggcagtt cggaccacgc cgctaccttc
     1621 gccaaatatc tgatcgagac ccgcacgggc gtcctgacct cgtcggcggg cttgtcggtc
     1681 agttcggtct atgccgcgac ccagaacatg gacgacgtgc tctatctggc catttcgcag
     1741 tcgggcaaga gccccgacct gttggcctct gtgcgcgccg ccaaggaggc tggagccttc
     1801 acgatcgccc tggtcaatga cacgtcctcg cctctggccg cactggtcga cgaggtcctg
     1861 ccgctgcacg cgggtcccga gctcagcgtc gcggccacga agtcctatat cgccgctcta
     1921 tcggccatca cccagctggt ggcggcctgg actggcgacg aggatctggc cgagggcctg
     1981 gccgccctgc ccgccggtct ggacgaggcc tggacgctgg actggacgcc cgccatcgag
     2041 cgtttgaagc tcgcccgcaa tctttatgtg cttggacgcg gcgtcggctt cggcgtcgcc
     2101 caggaagcgg ctttgaagtt caaggaaacc tgcggcctgc acgccgaggc tttcagcgcc
     2161 gccgaggtgc tgcacggccc gatggccctg gtgaaagccg gatttccagt gttggtcttc
     2221 gcccagaacg acgaaagccg gaggagcgtc gacgaaatgg ccgccagcct gaccgcgcgc
     2281 gacgccgacg ttcttctggt cggggcggga gaagtcgggg ccggcgtcct gcccgctctc
     2341 gcggcccatc cggtgatcga accgatcctg atgatccaga gcttctatcg catggccaat
     2401 gcgctctcga ttgcgcgggg ctatgatccc gacagcccgc cccatctcaa caaggtgaca
     2461 gaaaccgtct gatgcaggct gtactggtaa atggccgcat cctcgcggag tccggcttcg
     2521 tcgacggcaa gagcctggtg gtcgagaacg gccggatcgc cgcgatcctc aacgccgccg
     2581 acgctccggc ggacgctacg cgccacgacc tggacggcgg gctgctggtt cccggcttca
     2641 tagacaccca ggtcaatggc ggcggcggcg tgctgttcaa cgacgcgccc acggtcgaga
     2701 ccatcgccgc tatcggcgct gcccaccggc ccttcggcac caccggcttt ctccccaccc
     2761 tgatcagcga cgatctgacc gtcgtcgaca ccgccatgcg cgcggtcgag gcggcgatcg
     2821 aagccggcgt acccggcgtg ctggggatcc acatcgaagg cccgttcctc aatgcgaagc
     2881 gcaagggcat ccatgacgct ggcaagtttc gggtgatcga cgacgcggcg gcagcccttc
     2941 tgggctcgct gaaaatcggc aagaccctgg tgaccctggc gccggaaacc acgacgccgg
     3001 atacgatccg caagctgaca aacaacggcg tgatcgtcgc cgcgggtcac accaacgccg
     3061 cctacggcac catgcgcaag gcgctggcgg ccggattgac cggctttacc cacctgttca
     3121 atgccatgtc gccattaacc agccgcgagc ccggcgcggt cggcgcggcc ctggaaagcc
     3181 agaccgcctg gtgcggcctg atcgtcgatg gccgccacgt cgaccctgtc gtcctgcgca
     3241 tcgccctgcg cacccggccg ctggaccgtt tcatgctggt gaccgacgcc atgccgacgg
     3301 tcgggatgat cgacaagagc ttcgacctgc agggtcgcca gattcatgtc gtcaacgggg
     3361 tctgcgtcga cgcacatggc accctggcgg ggtccgacct cgacatggcc gccgccgtgc
     3421 gcaatgcgat ctcgatgctg aacctgactg tcgaggacgc cgtctcgctc gcctcggcct
     3481 ctccggcggc cttcttgggc ttggccggcc aacgcggccg tatcgccgtc ggtcaggctg
     3541 cagacctggt gctgttggac gacaacctga aggtccgcga gacctggatc gacggcgcct
     3601 ccagtcgctg atccgacaga ttgtacagtc gacatgaaaa aggccgccgg cgggagccag
     3661 cggccttctt cgttccagcg ctttagaaat tacatcttgt agcggacgcc gaacatgatc
     3721 gtacggccga agtggttgta ttcgtagtta cgatccgcct cgagatcggt gaagcgatca
     3781 cgatactcgt cagtcaggtt cacaccctca agcgagaact cgaggttgtc gttcaggcga
     3841 tagcgcatcg aggcatcaac attgatcgag ctgttgtagc cctcgaacac gttgcccgtg
     3901 ccactggtcc catcgataaa gccgctacga taggcggccg aggcgcgggc gctgaacttg
     3961 tcggcttcat agtagagcgt ggcgttgaac gcccggcgcg acagattgga caggcgctcg
     4021 gtcagggtca cagccggggc gagggtcggg aaggtcccga gggggttggt ggcggccccg
     4081 cggatgcccc gatattcgac gtcggagtcg accagggtca cattggcgat accgccaaag
     4141 ttcgaccaca gaccgggcag nnnnnnnnnn gaactcaacg cccttcagct tgccaccctg
     4201 gccgttggtg gtggtgttga tcgtccaggg ctgaccttcg gggttggcgg cggccggcga
     4261 actggccagg atcagggaca gcggcagacc cgtcgacgca taggtgcccg ttgaggacac
     4321 accgacgggg aagctttcga tatccttcat gaacaccgcg accgagaaga tcgactcgcg
     4381 ggcgaagtac cattctgccg agacgtcgta ggcggtggcc cggaacggat ccagttgcgg
     4441 attgcccgag gtgacccggt agttgaagcc atcgacactg ccaccggggg tcaggttacc
     4501 caggttgggg cgggtcatga ccttggcgat cgagccgcgc aggatcaggt cctcacgcgg
     4561 atagagagcc aggttacccg ctggcagcca gtcttcatac tgacgctcga tcgtcacagt
     4621 ctggccgttg ctgataccgg tcgaggactg ctcggtcttc atgtagcgca cgcccaggtt
     4681 cagggcgtag tccacgatat cgccgaagcg gctcttgacg tcgaactgca cgaagccgcc
     4741 ggtgtccttt tcggtgacgc cccgagtatt gcccgcctgc tggatcagcg gacgattgta
     4801 gaggtcaatc atcgagaccg cagcctggag gtttgtcacc acccagctgg tcgtcgtacc
     4861 ggccggcgcg tctgctccgg tcagctcgaa ctgctcggtc agcgccgctg taaccggcgc
     4921 accgtaggcg cccgccgcgc aggtgaaggc tcgacagtag gccgagtcgc gcgcgccctc
     4981 ccgcagacta aactcgaact cgcgataaac ggcgccggcc ctccaggtca gttcgttcgt
     5041 gatcgcatag tccccgttca acgccaccgt cttgaactcg ttggtattgt tggacggccg
     5101 gtcgcggaat tcggcgaact ggaagttcgt cggctcggtg acgctggtgc cgaacgccag
     5161 caccggcttc ttcatgtcgg tatagtcgta cttgtagccg gtggcgtcgc ggtcatcgaa
     5221 gatgatcgtg gtttcgtagg ggatgtcggc gtcggacttc gagaaaccgg ccaggccctg
     5281 gatcttgaac tggtcgttga actcgtgggt caggttggcg ctgaactggt agaaggtggt
     5341 gtccagctcg cgctcatagt gctcgatgcg cacccaggcg ttgtcgaacg tgcccttgat
     5401 caggttattg ttgttgtcga tcgtatagtc gcgaaccgag atcgagcgct cgttcgaacg
     5461 gaacaggacc tcggcccact tctcgttacg aatggcgtta tagcgcgaat agaggccatc
     5521 aactgacagt ttggtccggt cggtgggctg gtactggaag ctgcccgtca ggcccaggcg
     5581 ctcgcggtca tgggtgactt cgccatagcg cgggatacgg gggtggaagg ccagggccac
     5641 ctgattgcag gacgcgctcg gcacataggt cccgcccgcg ttctgggtgg tgaagcagtt
     5701 ggttccggcg accgagttga agcgcgcctg cgcccaacgc acggtgttgt tggtggattc
     5761 gatctgctcc tgcttcgagt aggccgccga catcgaggct gcccagacgc cgctgggggc
     5821 gcgataaccg atcagaccgg cgaggcgcgg gccaaggttc ttgctaaggt cattatactg
     5881 gccctgagcg ctcagggcga gggtcaggcc atccttgcca gcgatcggat taccggtgtt
     5941 caggtcgacg acggcgccca gcgacccttc gtcgagcgag gcctcggcgg tcttgtgcac
     6001 gaccagggag ttgaagagtt ctgaagcgaa aacgttgaag tcaaagccac gctcgcggtt
     6061 ggcgctggca ccgtccgagg tggtggcgat cgcctccatg ccattgacgc gaacgcgcgt
     6121 gaactgcgag cccaggccgc gaacggtgat ggcgcgacct tcgccgccgt cccgaacgat
     6181 cgagatgccg gggatgcgct gcagcgattc cgcgaggttc tggtcgggga acttggcgat
     6241 gtcttcggcg acgatcacgt ctacggccgc aaccgactgg cgcttgacgt tcaaggcctg
     6301 gtccagtgag cgccggaagc cggtgaccac cacttcctcg accacagcct cttcgcccgc
     6361 ggccggcgcg gtctgagccg atgcagctcc agccattgcg acagcgagcg caagggcccc
     6421 cgtggagaca ccaagcttca ggacgttgag acgtcctccg gaaatcttag acatgtatcc
     6481 tcctcccgtt cgacggaaac gacgctgtac gtcgcccggc ttcgaatcga tcgcgcttcc
     6541 ttccggcgc
//
LOCUS       DAOMEO010000024         6474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1666_length_6474_cov_2.488550, whole genome shotgun sequence.
ACCESSION   DAOMEO010000024 DAOMEO010000000
VERSION     DAOMEO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_6474_cov_2.488550"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLH31_01045"
     CDS             <1..769
                     /locus_tag="PLH31_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQR87867.1"
                     /translation="TAQGRVRQLVDKLDVLPNPISLFVDVGRDADSDAAISRIADAAL
                     ADLSGTPANARKAFVKRLQDVIIGIGGLIVAAPVMAIVAVAVRLDSPGPIFFRQRRHG
                     FNNEEILVWKFRSMRHEMADARAARQISANDDRVTKVGKFIRKTSLDELPQLFNVLRG
                     EMSIVGPRPHAVGMRTGDVESAKLVEQYAHRHRMKPGVTGWAAINGSRGPVDTPELVR
                     ERVALDVDYIERQSFWLDLYIIVMTIPCLLGDRSTVR"
     gene            778..>1914
                     /locus_tag="PLH31_01050"
     CDS             778..>1914
                     /locus_tag="PLH31_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQR87868.1"
                     /translation="MFWRGVLGYLPVNIAQGVVGLLTIVLFTRVLTPEEYGAYALAFS
                     ALSLTQTVLLTWSEAAMARFFAAREEDGRLPDHFATLYRLWGACALATPVIAAIVLYV
                     LPLDPPMKVALAAAFAAVIVKSLTKLSQERRRAAGEVSGAALLDVVQTVGGFALGLIL
                     ALVGLGGAAPLLGMGAISLLCLVFVLPTDLGRMRGGRFDRAMARDYATYGVPVALSLI
                     LALVLSTTDRFLLAAYLGEATVGVYHAGYSLGSRTLDVVFIWLGMAGGPALVSALERG
                     GPTALKDAAREQAAFMALLTLPAATGLALVAPSLVELMVGESLRAGAVKVVPWIAASG
                     WLSGVTTYYLLQAFTLGRRTPMLIAAMAIPALANLVLNLLLIPRF"
     assembly_gap    1915..1924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1925..2225
                     /locus_tag="PLH31_01055"
     CDS             <1925..2225
                     /locus_tag="PLH31_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQR87869.1"
                     /translation="DGALWATTASYGVGAVAAWALGRRAQCLPIPWDTLAKAGGACVL
                     MAAAVLILPSPGGILELILKASVGAAVYGGVVLGLNVDGLRGKLSGLARRKARPA"
     gene            2222..3154
                     /locus_tag="PLH31_01060"
     CDS             2222..3154
                     /locus_tag="PLH31_01060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR87870.1"
                     /translation="MTSRPSEIIIDNAAWASAAPRLSVLIPTHRDDPSALLRALDDPM
                     ALAEIIVLDDGSGDDALAKRIAGRIERLRLPARFIRLASNEGRAKGRNRLASHARAGH
                     FLFLDSDMLPDPENFLARWAALAEGENTPIAFGGFTLEQTPLRPEHALHRAMALKSDC
                     TPAPERAKSPEKHVFTSNLLVRRDIFETVAFDEGFTGWGWEDVEWAMRVGRQHPILHI
                     DNSATHLGLDTAPAMAAKYEQSAANFARVVSAHRDIVSTYPSYRVAKILKSFPARDIW
                     RPMLKSFALNEAAPTSLRAFAMRLYRAALYCEAV"
     gene            3154..3927
                     /locus_tag="PLH31_01065"
     CDS             3154..3927
                     /locus_tag="PLH31_01065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQR87871.1"
                     /translation="MQVSHDKVDAYEPDRTLKGKLRRRLIRLAHRRPARVKLERPMVS
                     FSFDDAPATACEAGARVLEARGLRGTYYFAGGLTGRDGPMGRFATGEDAARLHAAGHE
                     IGCHTFSHLDCGQSSREATLADINQNASALKSWGAGEPVSFAYPYGDVGAAAKTLLAG
                     RFKTVRALHHGLVANGADLNQAPAVGIEGEDGEAVALKWLDRAHARKAWLILYTHDVA
                     TAPSQWGCSVEVLERLVDGAMNAGFDVVTVAEGARRIGL"
     gene            complement(3924..5030)
                     /locus_tag="PLH31_01070"
     CDS             complement(3924..5030)
                     /locus_tag="PLH31_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQR87872.1"
                     /translation="MKPAALQAEPDQPARIFAPASDEKSPRVSFARIAVILSFLAYVA
                     AMGAAWWVVLQVVPAHVSFTVFGRTLDTESAHEHINNTALIFLLLPSALWIEAVVVGW
                     SKSSARQLIFAPTASLKTDLACFFLGQARVLDIFGRIMMLGASMISGAWIHDWLKTTT
                     GLSIGPAPMPLLLQVVFYFLVYTFFDYWTHRLDHTPRFWPLHRYHHSASDFGVITAGR
                     QHPAAFTGLFVVNLPMAILGAPADVMIYVNVLVVGIGFLIHSKIDANWGWIGRWVIQS
                     PNHHRLHHKLDMSHPTGHFAVAPIWDRLFGTWYGDADASIPIGVDTPYNHGLFVPRDM
                     LRDYWHFWIGLFGGRSDGPDSDIRLFQKRDAVKG"
     gene            complement(5117..6295)
                     /locus_tag="PLH31_01075"
     CDS             complement(5117..6295)
                     /locus_tag="PLH31_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HQR87873.1"
                     /translation="MLLLYSGGWALPLFGENADESAAGFMRVAYLPAYAVGFLLIALN
                     PRNMFRVLLRQPLLIILMLVVIGSTAWSIAPDVTTRRAFAVTCTTLGGLALAARYKWA
                     ELAEVVGATFIVLIVGSFVAALAVPSIGVMTELFPGAWRGLWMEKNGLGGLMALGFCL
                     LSAAALLNPQRAKLWWPFAGLALVLVLMSTSKTSLASLLVGAAALAFVLIARRSPAAG
                     AAITWTGVTGAVLLGGFLLVASDVFFALLGKDATLTGRTEIWSAAMRQIELRPWTGYG
                     YAAVWDNKSGWGPFAWITKDAGFQAHHAHNSWIEQWLGMGIIGLAAWGLFYLQTLALA
                     IIAVFRDRGALLVFPFLVVYTLVSLTESIAVVYNDFRWALFVAFAAKLAFSDREQERR
                     "
BASE COUNT         1131 a         2151 c         2124 g         1058 t
ORIGIN      
        1 aacagcgcag ggacgtgttc ggcaattggt cgacaagctg gacgtcctgc ccaacccgat
       61 cagcctgttt gtcgatgtcg gccgagacgc cgacagcgac gccgcgatct cgagaatcgc
      121 tgacgccgct ctcgccgacc tgagcggcac gccggccaat gcccgcaagg ctttcgtcaa
      181 gcgcctgcag gatgtgatca tcgggattgg cggcctgatc gtcgccgccc ccgtcatggc
      241 cattgtcgct gtcgctgtgc ggctcgacag ccccggcccc atcttcttcc gccagcgtcg
      301 ccacggtttc aacaacgagg agatcctcgt ctggaagttc cgctcgatgc gccacgaaat
      361 ggccgacgcc agggccgcac gccagatcag cgccaatgat gaccgcgtca ccaaggttgg
      421 caaattcatc cgcaagacca gcctcgatga gctaccgcaa ctgttcaatg tgctacgcgg
      481 cgagatgtcg atcgtgggcc cccgtccgca cgccgtgggt atgagaactg gcgacgtcga
      541 gtccgccaag ctggtcgagc aatacgccca ccgccaccgc atgaagcctg gtgttacggg
      601 ttgggccgca atcaacggct cgcgcggccc ggtcgacacc ccggaactgg tgcgcgagcg
      661 cgtcgcgctg gatgtcgact atatcgagcg ccagtctttc tggctggacc tctacatcat
      721 cgtcatgacc atcccctgcc ttcttgggga tcggtcgacg gtgcgctgag gcgggcgatg
      781 ttctggcgcg gcgtcctcgg ctatctgccg gtcaatatcg cccagggcgt ggtgggcctg
      841 ctgaccatcg ttctgttcac gcgggttctg acacccgagg aatacggcgc ctacgccctg
      901 gccttctcag cccttagcct gacccagacc gtcctgctga cctggtcgga agcggccatg
      961 gcgcgcttct ttgcggcgcg cgaggaggac ggccgcctgc cggatcactt cgccaccctc
     1021 tatcggctgt ggggcgcctg cgccctggcg acgcccgtca tcgccgccat cgtcctgtac
     1081 gtcctgcccc tcgatccgcc gatgaaagtg gctctggccg cggccttcgc ggccgtgatc
     1141 gtcaagagtt tgaccaagct gtcccaggag cggcgacgcg cggcgggcga ggtttcaggc
     1201 gcggcgctgc tggacgtcgt ccagaccgtc ggcgggttcg cgctgggctt gatcctggcc
     1261 ctcgtcggtc tgggcggagc cgcacctctg ctgggcatgg gcgccatctc gctattgtgc
     1321 ctggtattcg ttctgccgac cgacctggga cgcatgcggg gcggccggtt cgaccgcgcc
     1381 atggcccgcg actacgcgac ctacggcgtg ccggtggctc tgtcgctgat cctggccctg
     1441 gtactgtcga ccaccgaccg cttcctgctg gcggcctatc tgggtgaagc gaccgtaggc
     1501 gtctatcacg ccggctattc tctcggcagc cgcaccctgg acgttgtgtt catctggctg
     1561 ggcatggcgg gcggaccggc cctggtctcg gccctggagc gcggcggccc gaccgccctg
     1621 aaagacgccg cccgcgagca agccgcgttc atggccctgc tgacccttcc cgccgccact
     1681 ggcctggccc tggtcgcccc ctccctggtc gagctgatgg tcggcgagtc gctgcgcgcc
     1741 ggcgcggtta aggttgtgcc gtggatcgcc gccagcggct ggctgtctgg tgtgacgacc
     1801 tactatctgc tccaggcctt caccctgggt cgccgcacgc cgatgctgat cgcagcgatg
     1861 gcgatccccg ccctggccaa cctggtcctc aacctgcttc tgatccccag gttcnnnnnn
     1921 nnnnggacgg cgcgctgtgg gcgacaacgg ccagctacgg cgtcggggct gtggccgcgt
     1981 gggccttggg ccgtcgcgcc cagtgcctgc caatcccgtg ggacaccctg gccaaggccg
     2041 gcggcgcctg cgtcctgatg gcagctgctg tgctgatcct cccctcgccc ggcggaattc
     2101 ttgagctgat cctgaaagct tcggtcggcg ccgctgtcta cggcggcgtg gtgctgggac
     2161 tcaatgtaga cggtctgcgc ggcaagctct ccggccttgc caggcgaaag gcgcgtccag
     2221 catgacctcc aggccgagcg agatcatcat cgacaacgcc gcatgggcga gcgccgcgcc
     2281 gcgtctgtcg gtgctgatcc cgactcatcg cgacgatccc tcggccctgc tcagggcgct
     2341 ggacgatccg atggcgctcg ccgagatcat cgtgctggac gatggcagcg gcgacgacgc
     2401 cctggccaag cgtatcgccg gccggatcga gcgcctgcgc ctgcctgcgc ggttcatccg
     2461 cctggcctcg aacgagggcc gcgccaaggg gcgcaatcgc ctggccagcc atgcgcgcgc
     2521 cggccacttt ctgttcctcg acagcgacat gctgcccgat cctgagaatt ttctggctcg
     2581 ctgggcggcc ttggcagagg gggagaacac ccccatcgcc tttggcggct tcaccctgga
     2641 gcagacgccc ctgcgacccg agcacgccct gcaccgggcc atggcgctga agagcgactg
     2701 caccccggcg cccgagcgcg ccaagtcacc cgaaaagcac gtcttcacct ccaacctgct
     2761 ggttcgtcgt gacatcttcg agaccgtcgc attcgacgag ggctttaccg gctggggctg
     2821 ggaggatgtc gagtgggcca tgcgcgtggg ccgtcagcac ccgatcctgc acatcgacaa
     2881 ttcagccacc catctgggcc tcgacaccgc tccggccatg gccgccaaat acgagcagtc
     2941 agccgccaac ttcgcacggg tggtcagcgc gcaccgcgac atcgtcagca cgtatccgag
     3001 ctacagggtc gccaagatcc tgaagtcttt cccggcgcgc gacatctggc gcccaatgtt
     3061 gaagtcgttc gccctcaatg aagctgcgcc gacgtcgttg cgcgctttcg ccatgcggct
     3121 ttaccgggcc gccctctatt gcgaggccgt ctgatgcaag tgtcccacga caaggtcgac
     3181 gcctacgagc cggaccggac cctgaagggc aagctgcgtc gccggctgat ccgcctggct
     3241 caccgccggc cagctcgcgt gaagctggag cggcccatgg tgtcattcag cttcgatgat
     3301 gcgccggcca cagcctgcga agccggagcc agggtgctgg aagcgcgcgg cctgcgtggc
     3361 acctactatt tcgcaggggg actgacgggt cgcgacggcc ccatgggtcg cttcgcgacg
     3421 ggcgaggacg cggcgcgttt gcacgccgct ggtcacgaga tcggctgcca cactttttcg
     3481 cacctggatt gcggtcaatc ctcgcgggaa gccaccctcg ccgacatcaa ccagaacgct
     3541 tcggcgctta agtcctgggg cgcgggcgag ccggtcagct tcgcctaccc ctatggtgat
     3601 gtcggcgcgg cggcgaagac gctgctggct gggcgcttca agaccgtgcg cgccctgcac
     3661 cacgggctgg tcgccaatgg cgcggacctg aaccaggcgc cagccgtcgg gatcgagggc
     3721 gaggatggtg aggctgtcgc gttgaagtgg ctggatcgcg ctcacgcccg caaagcgtgg
     3781 ctgatcctct acacccacga tgtggcgacg gcgccctccc agtggggctg ttcggttgaa
     3841 gtgctggagc ggctggtgga tggcgcgatg aacgccggct tcgacgtggt caccgtcgcc
     3901 gagggcgcgc gccggatcgg gctttagccc ttcacagcgt cgcgcttctg aaacaggcgt
     3961 atgtccgagt ctggcccatc gctgcggccg ccgaacaagc cgatccagaa atgccagtag
     4021 tcgcgcagca tgtcgcgcgg cacgaacagg ccgtggttgt agggcgtatc caccccgatc
     4081 gggatcgagg cgtcggcgtc gccgtaccag gtgccaaaca gccggtccca gatcggcgcg
     4141 acggcgaagt ggccagtcgg atggctcatg tccagcttgt ggtgcagacg gtgatggttt
     4201 ggcgactgga tcacccagcg gccgatccaa ccccagttcg catcgatctt ggagtgaatc
     4261 agaaagccaa ttccgaccac caagacgttg acatagatca tcacgtcggc cggggcgccg
     4321 agaatggcca tcggcaggtt gaccacgaag agccctgtaa aggctgcggg atgctggcgg
     4381 cctgcggtaa tgaccccgaa gtccgacgcc gagtggtgat agcgatgcag cggccagaag
     4441 cggggcgtgt ggtccagcct gtgggtccag taatcgaaga aggtgtagac gaggaaatag
     4501 aacacgacct gcagaagcag cggcatcgga gcgggaccga tggacagtcc cgtcgttgtc
     4561 ttcagccaat catggatcca ggccccggaa atcatcgagg ccccgagcat catgatccga
     4621 ccaaagatgt cgaggacacg cgcctgaccc aggaagaagc aggcaagatc ggttttcagc
     4681 gaggcggtcg gcgcgaagat cagttggcgc gccgagcttt tggaccagcc gacgacgaca
     4741 gcctcgatcc acagggctga tggcagcaac aggaaaatca gggcggtatt gttgatgtgt
     4801 tcgtgcgccg actccgtgtc gagcgtacgg ccaaacaccg tgaaggacac atgggctgga
     4861 acaacctgca agaccaccca ccaggccgcg cccatggcgg cgacataggc caggaagctc
     4921 aggatgacgg ctatgcgcgc aaagctgacg cgcggggatt tttcgtctga cgctggcgcg
     4981 aagatccgcg caggctgatc cggctccgcc tgaagtgctg caggtttcac ttaagaggct
     5041 ccgccttctg tgacgcttga agtcagcaga gctagctcta ggtcaggcgc gttaaaaact
     5101 tcgttcggcg aaacggttaa cggcgttcct gttcccggtc cgagaaggcc agtttggcgg
     5161 cgaaggccac gaacagagcc cagcggaagt cattgtagac gacggcgata ctctcggtca
     5221 gcgacaccag agtgtagacc accagaaacg ggaagacgag caaggcgccg cggtcgcgaa
     5281 acaccgcgat gatcgccagg gccagggtct gaagatagaa gagcccccag gcagccaggc
     5341 caataatgcc catgcccagc cattgctcga tccaggaatt gtgcgcgtgg tgcgcctgga
     5401 agcctgcgtc cttggtaatc caggcgaacg gcccccagcc ggacttgttg tcccagacgg
     5461 cggcatagcc atagccggtc cagggtcgca gctcgatctg acgcatcgcg gcgctccaga
     5521 tctcggtccg gccggtgagc gtggcgtcct tgcccagcaa ggcaaagaag acatccgaag
     5581 cgacaagcag gaagccgcca agcaaaaccg cgccggtcac cccggtccag gtgatggccg
     5641 ctcccgcagc ggggctgcgc cgggcgatca gcacaaaggc cagggcggcg gccccaacca
     5701 gtagcgaagc cagggaggtc ttggacgtcg acatcagcac cagcactagc gccagccccg
     5761 caaacggcca ccagagcttg gcgcgctgcg ggttcagcag cgccgcggcc gacagcaggc
     5821 agaagcccag cgccataagg ccgcccagac cgttcttctc catccacaac ccgcgccagg
     5881 cgcccgggaa aagttccgtc atgacgccga tcgagggcac tgccagagcc gccacgaagc
     5941 tgccgacgat caggacgata aaggtggccc ccaccacctc ggccagctcg gcccacttgt
     6001 agcgcgcggc cagggccagc ccgcccagag tggtgcacgt caccgcgaag gcgcgccgcg
     6061 tggtgacgtc gggcgcgatc gaccatgcgg tcgaaccgat tacgaccagc atcaggatga
     6121 tcagtagcgg ctggcggagc aggacgcgga acatgtttcg cggattgagc gcgatcagga
     6181 ggaagccgac ggcataggcg gggagatagg ccacgcgcat gaagcccgcc gcggactcgt
     6241 cggcgttctc gccaaacagg ggcagcgccc agccgccgct gtagagcagc agcatggcga
     6301 ccgaggcgca gaagatcgcg atctggggca ggaggagcgg cgccctcccc ggcggcgtca
     6361 cgggaggatc gcccgcagga acgccggcgg ctccaccgaa acctgattga agaacacacc
     6421 ggcacaacga ccacccgccg tggcgagggc atcgcgtagc tgtgccggcg gctt
//
LOCUS       DAOMEO010000025         6459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1669_length_6459_cov_2.391162, whole genome shotgun sequence.
ACCESSION   DAOMEO010000025 DAOMEO010000000
VERSION     DAOMEO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1669_length_6459_cov_2.391162"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLH31_01080"
     CDS             complement(<1..257)
                     /locus_tag="PLH31_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR87874.1"
                     /translation="MEFELLPDPFKPPVRPAQGLNPARVWRMGWLAAIGMAAFATAFL
                     PGEFSWPVWAAFLAGAAPALLSLPLSGENLREDDHVQSILLV"
     gene            454..1239
                     /locus_tag="PLH31_01085"
     CDS             454..1239
                     /locus_tag="PLH31_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR87875.1"
                     /translation="MPEPASLDALPEYDASAARRAAAHERRRAALDDRKRSFLRMVSH
                     ELRTPLNAVIGFSEILSRELYGPLGSPQYREYAEIVHDSGLKLLKLVNQIVEIARLEG
                     HVTDLDLGPEGLDEAIEDAIEQLKGEIAQRQVTVTVPGSGALPAVMADSRGLRTMLGN
                     LLQNAITHSPVGGEVCIFVQALPGGVVEISIADQGLGVEPEELPRLLKPFEQGGSTLT
                     RTSEGAGLGLPIVDLLARAMHGALRLTSRPGAGLTAQLSLPEG"
     gene            1291..>1535
                     /locus_tag="PLH31_01090"
     CDS             1291..>1535
                     /locus_tag="PLH31_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQR87876.1"
                     /translation="MTRPIDAALEELADEFELLGDWEERYRYVIELGHGLAPLSDAER
                     SEANKVRGCASQVWLVTEPQADQTIRFRGDSDAHIVSG"
     assembly_gap    1536..1545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1632..1793)
                     /locus_tag="PLH31_01095"
     CDS             complement(1632..1793)
                     /locus_tag="PLH31_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87877.1"
                     /translation="MSILIVIIIALVLLGLALYVVQRSPIPSPVNWIIQLLLVVVAIL
                     FIGNRAGFF"
     gene            1886..>2709
                     /locus_tag="PLH31_01100"
     CDS             1886..>2709
                     /locus_tag="PLH31_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQR87878.1"
                     /translation="MTSDTPRSAPGAFRVAALYRFTRFEDPAALQGPLARACCAVGVR
                     GTLLLAREGINGTIAGTDAAIESVLAHIRALQGCADLQPKTAWADHMPFNRMKVRLKK
                     EIVTLGLPDLDPVANAGAYVEPANWNALIADPDVVVIDTRNDYEVAIGAFEHAVDPQT
                     SSFSAFPEWFRDYRAGLEQERGPNAPPLKVAMYCTGGIRCEKSTAFLKSEGIEDVFHL
                     KGGILDYLEQVPEPESLWRGECFVFDERVAVGHGLSPGTHGLCRGCRRPVSPEQRAS"
     assembly_gap    2710..2719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2720..2863
                     /locus_tag="PLH31_01105"
     CDS             <2720..2863
                     /locus_tag="PLH31_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87879.1"
                     /translation="CPACHHERDEASKARYAERHRQVARARELGIAHVGARVGEPQET
                     GED"
     gene            2932..3246
                     /locus_tag="PLH31_01110"
     CDS             2932..3246
                     /locus_tag="PLH31_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87880.1"
                     /translation="MVAYDSIYGAPPAAPKRSRAADKISSFLGRKPKDAGLGPIAPAP
                     DYALTPDQHGKITLAGKKIPLGLALLGVGIAAVLLVNPRTRGPVIAAATTAWGFFGKK
                     TA"
     gene            3303..3755
                     /locus_tag="PLH31_01115"
     CDS             3303..3755
                     /locus_tag="PLH31_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HQR87881.1"
                     /translation="MAHTNEDHIKLINGLVETTIDSADGYAEAAKDADTGRYKDLFEK
                     RAQERRTVATDLQDEVRRLGGAPKDDGTILAAAHRAFVNLRDSLSKGDEAVINEVESG
                     EDFIKAKYEKALEDNDVDVQTRAVIEKAWTSVKSGHDQMRDIKQALKH"
     gene            complement(3816..4550)
                     /locus_tag="PLH31_01120"
     CDS             complement(3816..4550)
                     /locus_tag="PLH31_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HQR87882.1"
                     /translation="MTEAMDLAVEHLAMRAARLLARPGAVIEAAPLGYAVRLGPNRRR
                     RPMLVIDETAFRALVRLTTLTPRKQGGWSLVFTPPARPAPPPGKPGVIEGEVTIEEPG
                     LGRTAFKANLGESPLAWLARRRDGQGRPWLTPVEAAAGERLREEFHRAGTVGRLTMNW
                     DAGPRVDGGGGRRLEPAERARAAKDRIAAALQAAGPGLREILEHVCLAGTALEAAERA
                     LGLPRRAGKTVLKLALQRLVLHYRMA"
     gene            complement(4547..4918)
                     /locus_tag="PLH31_01125"
     CDS             complement(4547..4918)
                     /locus_tag="PLH31_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR87883.1"
                     /translation="MLVQALEDIWAITPDPRRDRLTVAFVTHLVAMATDVSPGDISAH
                     KRTSVAAARARQIAIYLTHVTFHWPLARVAFAFGRDRTTCAHACHRIEDLREDAGFDA
                     KLVSLEACLRQAPRQILEGAR"
     gene            complement(5057..5905)
                     /locus_tag="PLH31_01130"
     CDS             complement(5057..5905)
                     /locus_tag="PLH31_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87884.1"
                     /translation="MLVTSRHQAALTARFLKPVAPMLEDLFLALRAETDLALGPDAPP
                     VYGKPYPYGYCLEITKDVQARLNARLRQPRHPAERAIKAYLNSGGAARRIWGVLRDRF
                     FQNAFQIGGLYIDVSNDTVDVSKPKVEILPMDQSGLEPVRDAAHFARIAERYWGVTFF
                     ANHALPSLAPLFPMIGVDARGKARLYSDIRYMVNLFRSSQFVQAECWLEDGPAPSMAL
                     IRAIRARCPADLLAANPVATQEAALQACRSARAEGRASDDDWLEARARDLLAIGRSPI
                     PVLERG"
     gene            complement(5986..>6459)
                     /locus_tag="PLH31_01135"
     CDS             complement(5986..>6459)
                     /locus_tag="PLH31_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQR87885.1"
                     /translation="ILAYVHSLGVAVALDFNYRPALWPSPEAARSALDGVVPSCRYIS
                     LSSEDSRPLYGREAEDLAAEWAAPGAEVVARDESHAVNIHRPEGRTATPAPDRVKAVD
                     TTGAGDSFNAAYLSSRLAGLPIETAVARAHRLAAIVVGCRGAIAPHGTRLRLDVP"
BASE COUNT         1187 a         2175 c         2075 g         1002 t
ORIGIN      
        1 accagcagga ttgactgtac gtggtcgtcc tcgcgcaggt tttcgcctga aagcggtaga
       61 gacagcagcg ccggcgcggc gcccgccagg aacgccgccc agacgggcca gctgaactcg
      121 cccggcagaa acgctgtcgc gaaagccgcc atgccgatgg ccgccagcca gcccatacgc
      181 catacgcgtg ctgggtttag cccctgcgcg gggcgcacgg gcggcttgaa cgggtccggt
      241 agcagttcga attccaaacc gggcgacttc tttcaatgca acgccgagga gcgctgtcgc
      301 caaaccttaa cgcgcctgag cccatccctc cagaccactc aatcggcgag cgatcattcc
      361 tggtgacgac ctgtggacgg aaacggattc gtaagcgcct tgggagcaag gtccaggctg
      421 cacgaatgcg gacagaatgt cccggagcct atcatgccag aaccggcgtc cctcgacgca
      481 ttgcccgaat acgacgcctc cgcagcgcga cgcgccgcgg cccatgaacg tcgccgggct
      541 gcgctggatg accgcaagcg atcgtttctg cgcatggtca gccatgaact gcgcaccccg
      601 ctcaacgcgg tgatcggctt ttccgagatc ctgtcccgcg aactctatgg cccgctgggc
      661 tcgccccagt accgggagta tgccgagatc gtgcatgaca gcgggctgaa gctgctgaag
      721 ctggtcaacc agatcgtcga gatcgcccgc ctggaagggc atgtcacgga tctggatctc
      781 ggccccgaag ggctggacga agccattgaa gatgcgatcg aacagctgaa gggcgaaatc
      841 gcccagcgac aggtgaccgt cacggttcct ggcagtggcg cgctgcctgc ggtgatggcc
      901 gacagccggg gcctgcggac catgctcggc aatctgctgc agaacgccat cacccactcg
      961 cccgtcggtg gtgaggtctg catctttgtt caggcccttc cgggcggggt ggtcgagatt
     1021 tccatcgccg atcagggact cggcgtcgaa cccgaggaac tgccacgcct gctgaagccc
     1081 tttgaacagg gcggctcgac gctgacccgg accagcgagg gtgcgggcct tggcctgccg
     1141 attgtggatt tgctggctcg ggccatgcat ggcgctctgc gcctgacctc ccgccccggc
     1201 gccggcctga cggcacaatt gagcctgccc gaaggctgag gcttaccagc agaccttgcc
     1261 agcagatggt cgcgccccta agtggcggcc atgacacgcc ccatcgacgc cgcccttgag
     1321 gaactcgctg acgagttcga actgctgggc gactgggaag aacgctatcg ctatgtgatc
     1381 gaactcggcc acggcctggc gccgctcagc gacgccgaac ggtcagaagc caacaaggtg
     1441 cgcggctgcg ccagccaggt gtggctggtc accgaaccgc aggccgacca gacgatccgc
     1501 ttcaggggcg acagcgacgc gcacatcgtg agcggnnnnn nnnnngactc aaggcgatgg
     1561 tcgcccgcat tcagcgcgac gcccaggcct tgacggccta gggacgcgcc ttgccgggct
     1621 aagccaatgg cctagaagaa gcctgcgcga ttaccgatga acaggatcgc gaccaccacc
     1681 agaaggagct ggataatcca gttcactggc gacggaatcg gcgaccgctg cacgacataa
     1741 agggcaagtc cgaggaggac cagggcgatg atgatgacga tcaggatgct catgtcagct
     1801 taaccgctat cgcgggtgtt ggttccccga cagttgcgac gaatacttga gacacaccgt
     1861 gtaagcgcct atggcctcgc ccctgatgac ctccgacacc ccaagatcag cccccggggc
     1921 ctttcgcgtg gccgcgctct atcgcttcac gcgttttgaa gacccagccg ccctgcaggg
     1981 cccgctggcc agggcctgct gcgccgtggg cgtgcggggc acgctgctgc tggcccgcga
     2041 ggggatcaat ggcacgatcg cgggaaccga tgccgccata gagagcgtgc tggcccatat
     2101 tcgcgccctg caaggttgcg ccgatcttca gcccaagact gcctgggccg atcatatgcc
     2161 cttcaaccga atgaaggttc ggctgaagaa agagatcgtc accctgggcc tcccggatct
     2221 cgaccccgtc gccaatgccg gggcctatgt cgagcccgcc aactggaacg ccctgatcgc
     2281 cgatcccgac gtcgtggtga tcgacacccg caacgactac gaggtcgcca tcggcgcctt
     2341 cgagcacgcg gtcgatccgc agacctccag cttctcggcc tttcccgaat ggttccggga
     2401 ctatcgcgcc ggactggagc aggagcgcgg tccgaatgct ccgccgctca aggtggccat
     2461 gtactgcacc ggcggcatcc gctgcgagaa gtccacggcc ttcctgaaat ccgagggtat
     2521 cgaggacgtc ttccatctga agggcggcat tctcgactat cttgagcagg tccccgaacc
     2581 tgaaagcctt tggcgcggcg aatgcttcgt gttcgacgag cgcgtggcgg tcggtcacgg
     2641 tctgtctccc gggacccacg gcctatgccg gggttgccgc cgaccggtca gcccggagca
     2701 gagggcctcn nnnnnnnnnt gcccggcctg tcaccatgag cgcgacgaag ccagcaaggc
     2761 tcgttacgcc gaacgccaca ggcaggtcgc ccgcgctcgc gaactgggca tcgcccatgt
     2821 cggcgccagg gtcggcgagc cgcaggaaac cggcgaagac tgacacaggc gagggatcgc
     2881 ctcgccgttc atcgcgttat cggaggcgaa cccacgccaa ggagcctccc catggtcgca
     2941 tatgactcca tctatggtgc gccgcccgcc gcgcccaagc gttcgcgcgc cgctgacaag
     3001 atctccagct tcctgggccg caagccgaag gacgccggtc tgggtccgat cgctccggcg
     3061 cccgactacg ctctgacgcc cgatcagcac ggcaagatca ccctggcggg caagaagata
     3121 ccgctgggcc tggcccttct gggtgtcggt atcgccgcgg tgctgctggt caatccgcgt
     3181 acgcgtggcc cggtgatcgc tgcggctact accgcctggg gcttcttcgg caagaagacc
     3241 gcctgaccca ccacgcctca cccccacgcc actctcgctg atcccccgca aaggaggccc
     3301 ccatggccca taccaacgaa gaccacatca agctcatcaa cggcttggtc gagaccacca
     3361 tcgacagcgc cgacggctat gccgaggccg ccaaggatgc ggataccggc cgctacaagg
     3421 acctgttcga gaagcgcgct caggagcgtc gcaccgtcgc gaccgacctg caggacgaag
     3481 tccgtcgcct cggcggcgcg ccgaaggacg acggcacgat cctggccgcc gcccaccgcg
     3541 cctttgtcaa tctccgcgac agcctgtcca agggcgacga ggcggtgatc aacgaggtcg
     3601 agtccggcga agacttcatc aaggccaagt acgaaaaggc tctggaagac aacgatgtcg
     3661 acgtccagac ccgcgctgtg attgaaaagg cctggacctc ggtgaagtcc ggccatgacc
     3721 agatgcgcga catcaagcag gcgctgaagc actaagacgt caccgcttgt gaagctgatg
     3781 aagggcggtg ccgaaaggtc ccgccctttt cgcgttcagg ccatgcggta atgcagcacc
     3841 agacgctgga gggccagttt cagcaccgtc ttgcccgcac gacgcggcag gcccagcgcc
     3901 cgctcagcag cctccagagc cgttcccgcc agacagacat gttcaaggat ctcgcgcagg
     3961 ccggggcccg ccgcctgcag cgcggcggcg atgcggtcct tggccgccct ggcgcgttcg
     4021 gcgggctcca ggcggcgccc gcctccgcca tcgacgcgcg gcccggcgtc ccagttcatg
     4081 gtcagccggc ccaccgtacc cgccctatga aactcctcgc gcaggcgttc tccggccgcc
     4141 gcctccacag gcgtgagcca gggccgcccc tggccatccc gccgccgcgc cagccaggcc
     4201 agcggggact cgccgagatt ggccttgaag gccgtacggc ccaacccggg ttcttcgata
     4261 gtgacttcgc cttcgatgac gcccggcttt cccggcggcg gcgccggtct ggcgggcggc
     4321 gtgaacacca gcgaccagcc gccctgcttg cggggtgtca gggtggtcag gcgtacgaga
     4381 gcccggaagg cggtctcgtc gatcaccagc atgggccgcc gccgacggtt gggtcccaag
     4441 cgcacggcgt accccagggg cgccgcctcg atcacagcgc ccggtctggc cagcaggcgg
     4501 gcggcgcgca tcgccagatg ctcgacagcc aggtccatcg cctcggtcat cgcgcgccct
     4561 ccaggatctg gcgcggcgcc tggcgcaaac aggcttcgag ggaaaccagc ttggcgtcga
     4621 aaccggcgtc ctcgcgcagg tcttcgatcc tgtggcaggc gtgggcgcag gtcgtgcggt
     4681 cgcgcccgaa ggcgaaggcc acccgggcca gcggccagtg gaaagtgaca tgggtcaggt
     4741 agatggcgat ctgccgcgcc cgggccgcag cgacgctggt gcgcttgtgg gcggagatgt
     4801 cgccgggcga gacatcggtg gccatggcga ccagatgggt gacgaaggcg accgtcagac
     4861 gatcgcggcg gggatcgggc gtgatcgccc agatatcctc aagagcttga accagcatgg
     4921 cgtcctctaa atcggtggcg cgatccagcg accacgactc gatgttaggc ttaatttctg
     4981 ctggtgagga taattttcct atcgtgacgc acaagtagga aaataagccg ttgaaaatcc
     5041 tacgtccgaa tctgtcctat ccccgttcga gcacggggat aggcgatcgt ccgatggcca
     5101 gaagatcgcg cgcgcgcgcc tccagccaat catcatcgga cgcccgcccc tccgcccgcg
     5161 cggatcgaca ggcctgaagc gcagcctcct gcgtagccac cggattggcc gccagcagat
     5221 ccgcgggaca acgggcccgg atcgcccgga tcaaggccat ggacggagcg ggtccgtcct
     5281 ccagccagca ctccgcctga acgaactggc tcgagcggaa caggttgacc atgtagcgaa
     5341 tatccgaata gaggcgggcc ttgcctcggg cgtccacccc aatcatcgga aacagaggcg
     5401 ccagactggg cagggcatgg ttggcgaaaa acgtcacccc ccagtatcgc tccgcaattc
     5461 tggcaaaatg ggcggcatcg cggaccggct cgagaccaga ctggtccatc ggcaggatct
     5521 cgaccttggg cttgctgaca tcgacggtat cgttggagac gtcgatataa agaccgccta
     5581 tctggaaagc gttctggaaa aaacggtccc gcagcacgcc ccagatccgc cgcgccgcgc
     5641 cgccggagtt cagataggcc ttgatcgccc gctcagcggg atgtctaggc tgtctcaacc
     5701 gagcattcag tcgcgcctgc acatccttgg tgatttccag gcaatagccg tagggatagg
     5761 gcttgccgta gacaggcggc gcatccggcc caagcgccag gtcggtttcg gcgcgcagag
     5821 ccaggaacag gtcctcaagc atcggcgcga cgggcttcag aaaccgggca gtcagagcag
     5881 cctgatgccg gctcgtgacg agcatacgcc tactctccac tcgagctcca ccacgcgtac
     5941 tgagcgccct gtcgcccttc cttagccgac cacgcccgag agtcgctatg ggacatcaag
     6001 gcgcagtctg gtcccatgag gcgcgatggc cccgcgacag cctacaacga tagcggccaa
     6061 ccggtgcgcc cgcgccacgg cggtctcgat cggcaggccg gccagacgcg acgacaggta
     6121 ggcagcgttg aaactgtcgc ctgcgcccgt ggtgtcgacc gccttcaccc tgtccggcgc
     6181 gggggtggcg gtgcggcctt cggggcgatg aatgttgaca gcgtggctct cgtcgcgggc
     6241 tacgacctcg gcgcccgggg ccgcccattc agcggctagg tcctcggcct cgcgaccata
     6301 gagcgggcga gaatcctcgc tgctcagcga gatgtagcgg caggacggga ccacgccgtc
     6361 gagggcgctg cgggccgcct cggggctggg ccacagggcc gggcgatagt tgaagtctag
     6421 cgccacggcg acgcccagcg aatggacata ggccaggat
//
LOCUS       DAOMEO010000026         6414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1678_length_6414_cov_2.475075, whole genome shotgun sequence.
ACCESSION   DAOMEO010000026 DAOMEO010000000
VERSION     DAOMEO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1678_length_6414_cov_2.475075"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="argE"
                     /locus_tag="PLH31_01140"
     CDS             <1..978
                     /gene="argE"
                     /locus_tag="PLH31_01140"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HQR87886.1"
                     /translation="PAVEGGVVLSGHTDVVPVDGQPWSADPFVLTEKDGRLYGRGTCD
                     MKGFLALALAAAPDLAAATLSRPVHLAFSYDEEVGCLGAPDMIEVIARDVPRPALVVV
                     GEPTDMVAVSGHKGIASFRVTVSGREAHSSLTHLGVSANMAAIKLMAGLVELSERLER
                     EADPASPFTPKGATLTIGQVNGGTAVNILARECVFIFDLRTPPGLDPMAILADFFARA
                     EAMDVELKARAPEAGVRVERRSLTPSFAPEVDGVAEAFARRMAGDNGPGRVVPYAAEA
                     GQFQGAGFSTVICGPGSIDQAHQPDEFVEISQMQRGAAFMARLIEDLSF"
     gene            complement(1104..2126)
                     /locus_tag="PLH31_01145"
     CDS             complement(1104..2126)
                     /locus_tag="PLH31_01145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR87887.1"
                     /translation="MPSVTADDKSTPTVSVIIVSYESGSTLERCLAGLKAQTFTDFEI
                     LLVDNASTDGAPQAAVAADPSIRFLEPGSNLGFAAGNNLAVRSARGRWLALLNPDAYP
                     EPGWLAALMDGVARHPDVESFASLQTSADDPGVLDGAGDNMTSAGIPFRGGYGRKLPT
                     NLPEGEVFSACGAAMLIDRALFTELGGFDERFFCYCEDVDLGYRLRLTGRTTLLLPAA
                     RVAHVGSASTGVRSDFAIFHGSRNRVWTFLKNTPPLLLWLTAPLHVAVTGALLLLHLR
                     RGDAGPAVRGIRAALKKPALDAVLADRKAVQARRKASSGAILRVMSIDPAAFIGRRFV
                     IRKSQL"
     gene            2160..2723
                     /gene="rfbC"
                     /locus_tag="PLH31_01150"
     CDS             2160..2723
                     /gene="rfbC"
                     /locus_tag="PLH31_01150"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQR87888.1"
                     /translation="MKITPLAIPEVLLITPARHGDERGWFSETFRQSALEEAGFTRPF
                     VQDNHVRSTTRGIQRGLHYQKPPHAQDKLLRCVVGAIFDVAVDIRKGSPTYGQWVGAE
                     LSAENRQQLLVPQGFAHGYCTLTDDCEVLYKCTDYYTPSAEGGVRWSDPAIGIDWTIP
                     NAQIGANARDEGAPLLAEIDSPFVYGE"
     gene            2720..>3163
                     /locus_tag="PLH31_01155"
     CDS             2720..>3163
                     /locus_tag="PLH31_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR87889.1"
                     /translation="MTGFTDLFWTSGEGLPLHARDYAPMGVHGGEESGLPVICIHGLT
                     RNARDFEDLAPRIAARGRRVLAVDVRGRGLSAWDPQPLNYHPGTYAGDIVALLAATGI
                     DKAVFVGTSMGGLITMVLASFKPEAIAAVVLNDVGPELSPIGLTRI"
     assembly_gap    3164..3173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3174..3590
                     /locus_tag="PLH31_01160"
     CDS             <3174..3590
                     /locus_tag="PLH31_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87890.1"
                     /translation="DEAVAYARRINQAAFPAYGDADWDRFARRFFDEKDGGYVLACDP
                     DISAPIKAAAAAGQEGGAALAPDMYPLFRALTKDRPVLLVRGGISDLIDPPIVERMCA
                     AAPDMAVVEVPGVGHAPMLTEPEAWSAIADLLDRAP"
     gene            3675..4877
                     /locus_tag="PLH31_01165"
     CDS             3675..4877
                     /locus_tag="PLH31_01165"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HQR87891.1"
                     /translation="MTLDLAAIQAAAGRLQGHIERTPCRYSKTLSKITGAEVWVKFEN
                     LQFTAAYKERGALNKLMLLSDAQRAKGVIAASAGNHAQGLAYHGARLGVPVTIVMPKT
                     TPFVKVQHTRDFGATVIIEGETYDDANAHALRLREEQGLTFVHPFDDYDIMAGQGTIA
                     LEMLEDVPDLEMLPVPIGGGGLISGVATAAKALKPDIRIIGCEPAMYPSFTAKMRGIT
                     AHCGGQTIAEGVAVKTVGELTYGVARPLIDDVLLLEEPHIEQAVALYCNVEKTIAEGA
                     GAASLAALLAYPERFRGKKCGLILCGGNIDTRLLASVLTRELVRAQRLVSLRIVGDDR
                     PGLLSTVAHVIGSMGANIIEVNHNRLALDVPAKGAEFDITIETRDAQHTQEIIDALRE
                     KGYPPRSV"
     gene            complement(5008..5964)
                     /locus_tag="PLH31_01170"
     CDS             complement(5008..5964)
                     /locus_tag="PLH31_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HQR87892.1"
                     /translation="MTQKIIFDTDPGIDDAMALLFIEASPALDLLAITTIFGNADIET
                     TTRNALYLKQRFGLSAPVYKGTDKPLTRPRNPSPTFVHGENGLGDVELTGLVPAQPEA
                     KPAYQAIIDIARQHPGEVTLVAVGPLTNLALALKADPEVATLLKAVVIMGGAFGVAGK
                     PGNVTPVAEANIWNDPEAADLVFTAPWALTAVSLDVTTQVVMSPAYMDALEASAGPAG
                     AFLNAISKPYAAFYGSRDGVSGCCVHDAAAVAFVIAPSLFEVRRGSVRVVTEGITLGQ
                     TCQKAEGELFGPSAWDDQPIQAVTVAVDEAGLLKLYAETMRA"
     gene            complement(5974..>6034)
                     /locus_tag="PLH31_01175"
     CDS             complement(5974..>6034)
                     /locus_tag="PLH31_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87893.1"
                     /translation="VSILQAGADQRLAPTTESR"
     assembly_gap    6035..6044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6045..6389)
                     /gene="hpxZ"
                     /locus_tag="PLH31_01180"
     CDS             complement(<6045..6389)
                     /gene="hpxZ"
                     /locus_tag="PLH31_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HQR87894.1"
                     /translation="MIVNDPAALAEVTAAVEAYETALMDNDVEALDGAFWNSPHTVRM
                     GVTENLWGFDQIAAFRVGRAGGSPPRTRLRTEITTFGADFAIANVAFQRDGSDKIGRQ
                     SQTWIRTGDGWKV"
BASE COUNT         1010 a         2193 c         2179 g         1012 t
ORIGIN      
        1 ccggcggtgg agggcggtgt ggtgctgtcg ggccataccg acgtggtgcc ggtcgatggc
       61 cagccgtggt cggccgatcc gttcgtgctg acggagaagg acggccgcct ctacggccgg
      121 gggacctgcg acatgaaggg gttcctggcc ctcgccctgg ccgccgcgcc ggacctggcg
      181 gccgcgacgc tgagtcggcc ggtgcacctg gccttctcct acgacgagga ggtcggctgc
      241 ctcggcgcgc ccgacatgat cgaggtcatc gcccgtgatg tgccccgtcc cgccctggtg
      301 gtggtcggcg agcccaccga catggtcgcc gtcagcggcc acaagggcat cgcctcgttc
      361 cgggtcaccg tttccggccg cgaggcccat tccagcctga cccatctggg cgtctcagcc
      421 aacatggcgg cgatcaagct gatggctggc ctggtggagc tttcggagag gctggagcgc
      481 gaggccgatc ccgcctcgcc gttcaccccc aagggcgcga ccctgaccat cggccaggtc
      541 aatggcggca cggccgtcaa catcctggcc cgcgaatgcg tgttcatctt cgatctgcgc
      601 accccgccgg gcctggatcc gatggcgatc ttggccgact tcttcgcgag ggccgaagcg
      661 atggacgtcg aactgaaggc tcgcgcgccc gaggccggcg tcagggtcga gcgccgctcg
      721 ctgacgccgt cgttcgcccc cgaggtcgac ggcgtcgccg aggccttcgc gcggcggatg
      781 gccggcgata atgggccggg ccgggtcgtg ccctacgccg cggaggccgg gcagtttcag
      841 ggcgcggggt tttccacggt gatctgcggt ccaggctcga tcgaccaggc gcaccagccc
      901 gatgagttcg tcgagatctc ccagatgcag cggggggcgg cgttcatggc gcggttgatc
      961 gaggatcttt ccttctagat ctccgtcatc ccgcgacttg ttcgcgggac ccatgtttca
     1021 gcttctacgt gaaagttcgt cctgctccgc acttgcggcg gacagatggg tcccgcgaac
     1081 acgtcgcggg atgacgggaa aatttagagc tgcgatttcc gaatgacaaa ccgccgcccg
     1141 ataaacgccg ccgggtcgat cgacatcacc cgcaggatcg cgccggacga cgcctttcgc
     1201 cgcgcctgaa ccgccttgcg gtcggccagc accgcgtcga gcgccggctt cttcagcgcc
     1261 gccctgatcc cccgcaccgc cggcccggcg tcgccccggc gcaggtggag cagcagcagg
     1321 gcgcccgtca ccgccacatg cagcggcgca gtcagccaca gcagcagcgg cggggtgttc
     1381 ttcaggaagg tccagactcg gttgcgcgag ccgtggaaga tggcgaagtc cgaccgcacc
     1441 ccggtgctgg ccgagccgac atgggccacg cgggcggcgg ggaggagcag ggtggttcgc
     1501 ccggtcagcc gcaggcggta acccaggtcg acatcctcgc agtagcagaa gaaccgctcg
     1561 tcgaagccgc ccagctccgt aaacagcgcc ctgtcgatca gcatcgccgc gccgcaggcc
     1621 gagaacacct cgccctcggg caggttcgtc ggcagcttgc ggccatagcc gccccggaag
     1681 ggaataccgg ccgaggtcat gttgtcgccg gccccgtcca gcacgccggg atcatcggcc
     1741 gaggtctgca gcgaggcgaa gctctcgacg tccgggtggc gcgcgacgcc gtccatcagg
     1801 gcggccagcc aaccgggctc gggataggcg tcgggattga gcagggccag ccagcggccc
     1861 cgcgccgacc tcaccgccag attgttgccg gccgcgaaac ccaggttcga gccaggctcc
     1921 aggaaccgga tcgacggatc ggccgcgacc gccgcctggg gcgcgccgtc ggtcgaggcg
     1981 ttgtcgacca gcaggatttc gaaatcggtg aaggtctggg ctttcagccc cgccaggcag
     2041 cgttccaggg tcgaaccgct ctcatacgag acgatgatca cgctgaccgt cggcgtgctc
     2101 ttgtcatcgg cggtcacaga cggcatcggt ggcggacttg tccctggagc gttctggtca
     2161 tgaaaatcac cccgctggcg attcccgagg tcctgcttat cacgcccgcc cgccatggcg
     2221 acgagcgcgg ctggttttcc gagaccttcc gccagtcggc gctggaggag gccggcttca
     2281 cccggccgtt cgtgcaggac aaccatgtgc gctcgaccac gcgcggcatc cagcgcggcc
     2341 tgcactatca gaagcccccg cacgcccagg acaagctgct gcgctgcgtg gtcggcgcga
     2401 ttttcgatgt ggccgtggat atccgcaagg gctcgccgac ctatggccag tgggtcggcg
     2461 ccgagctgtc ggccgagaac cgccagcaac tgctggtgcc ccagggcttc gcccacggct
     2521 actgcaccct gaccgacgac tgcgaggtgc tctacaagtg caccgactat tacaccccgt
     2581 cggccgaagg cggcgtgcgc tggagcgacc cggccatcgg catcgactgg acgatcccca
     2641 acgcgcagat cggcgccaac gcccgcgacg agggcgcgcc tttgctcgcc gagatcgact
     2701 cgccgttcgt gtacggcgaa tgaccggctt taccgacctc ttctggacct caggcgaagg
     2761 cctgccgctg cacgcccgcg actacgcgcc aatgggcgtt catgggggcg aggaatcggg
     2821 tctgccggtg atctgcatcc acggcctgac gcgcaacgcg cgcgacttcg aggacctggc
     2881 gccgaggatc gccgccaggg gccggcgcgt cctggccgtc gacgtgcgcg gccgtggcct
     2941 gtcggcctgg gatccacagc cgctgaatta ccaccccgga acctatgccg gcgacatcgt
     3001 cgccctgctg gcggcgaccg ggatcgataa ggcggtgttc gtcggcacca gcatgggcgg
     3061 gctgatcacc atggtgctgg cgtccttcaa gccggaagcc atcgcggcgg tcgtactcaa
     3121 cgatgtcggc cccgaactgt cgcccatcgg cctgacccgc atcnnnnnnn nnngacgagg
     3181 ccgtcgccta tgcgcgccgt atcaaccagg cggcctttcc ggcctatggc gacgccgact
     3241 gggaccgctt tgcccgccgg ttctttgatg agaaggacgg cggctacgtc ctggcctgcg
     3301 atccagacat ttccgcgccg atcaaggcgg cggccgccgc cggacaggag ggcggggctg
     3361 ccctggcccc ggacatgtat ccgctgttcc gcgccctgac caaggaccgc ccggtgctgc
     3421 tggtgcgcgg cggcatttcc gacctgatcg acccgccgat cgtggaacga atgtgcgccg
     3481 ctgcgcccga catggcggtg gtcgaggtcc ccggcgtcgg tcacgcgcca atgctcaccg
     3541 aaccggaagc ctggagcgcc atcgccgatc tgctggatcg cgcgccctga agccgcaagg
     3601 gcgtgcggag cgcgggtcga ctgtgctaga cgcgcccgga cgccgccagt gttccgcgtc
     3661 cagataggtt gtccatgact ctcgacctcg ccgccatcca ggccgccgcc ggccgtctcc
     3721 agggccatat cgagcgcacg ccgtgccgct attccaagac cctgtcgaag attaccggcg
     3781 ccgaggtgtg ggtgaagttc gagaacctgc agttcacggc cgcctacaag gagcgtggcg
     3841 cgctcaacaa gctgatgctg ctctccgacg cccagcgcgc caagggcgtc atcgccgcca
     3901 gcgccggcaa ccacgcccag ggcctggcct atcacggcgc gcggctgggc gtgccggtga
     3961 caatcgtcat gcccaagacc acgccgttcg tgaaggtgca gcacacccgc gacttcggcg
     4021 cgacggtgat catcgagggc gagacctatg atgacgccaa cgcccatgcc ctgcggctgc
     4081 gcgaggaaca gggcctgacc ttcgtccacc cgttcgacga ctacgacatc atggcgggcc
     4141 agggcaccat cgccctggaa atgctggaag acgttccgga tctcgaaatg ctgcccgtgc
     4201 cgatcggcgg cggcggtctg atcagcggcg tggccacggc ggccaaggcc ctgaagcccg
     4261 acattcgcat catcggctgc gagccggcca tgtatccgtc cttcaccgcc aagatgcgcg
     4321 gcatcaccgc ccattgcggc ggccagacga ttgccgaggg cgtggcggtc aagaccgtgg
     4381 gcgagctgac ctatggcgtc gcccgcccgc tgatcgacga cgtgctgctg ctggaagagc
     4441 cgcatatcga gcaggccgtg gcgctgtact gcaacgtgga aaagaccatt gctgaaggcg
     4501 cgggggcggc ctcgctggcg gcgctgctgg cctatcccga acgcttccgg ggcaagaagt
     4561 gcggcctgat cctgtgcggg ggcaatatcg atacgcggct gctggcctcg gtgctgaccc
     4621 gcgaactggt ccgcgcccag cgcctggtgt ccctgcgcat cgtcggcgac gaccgtccgg
     4681 gcctgctgtc gaccgtggcc cacgtgatcg gctcgatggg ggctaacatc atcgaggtca
     4741 accacaaccg cctggccctg gacgtgccgg ccaagggcgc ggagttcgac atcaccatcg
     4801 aaacccgcga cgcccagcac acccaggaaa tcatcgacgc cctgcgcgag aagggctatc
     4861 cgccgcggtc ggtttaaggc gcgagagatg ctcccctttt gggggagctg tcgcggagcg
     4921 actgaggggg ctaatgcggc ttaggccgag ctggccccct ccggccctct gggccacctc
     4981 ccccagaagg ggaggatcta acaagagcta cgccctcatc gtctccgcat acaacttcaa
     5041 cagccccgcc tcatccaccg ccacggtcac cgcctggatc ggctggtcgt cccaggcgct
     5101 ggggccaaac agttcgccct cggccttctg gcaggtctgg cccagggtga tgccctcggt
     5161 caccacccgc accgacccgc gacgcacctc aaagagcgac ggggcgatca cgaacgccac
     5221 cgccgccgcg tcgtgcacgc aacagccgct gacgccgtcg cggctgccgt agaaggccgc
     5281 atagggcttg gaaatcgcgt tcaggaacgc gccagccggt ccggccgagg cttccagcgc
     5341 gtccatatag gccggcgaca tcaccacctg ggtggtgacg tcaaggctga ccgcagtcag
     5401 ggcccacggc gcggtaaaga ccagatcagc ggcttccgga tcgttccaga tattggcctc
     5461 ggcgacgggg gtcacattgc ccggcttgcc ggccacgccg aacgcgccgc ccatgatcac
     5521 cacggccttc agcagggtgg cgacctcggg atcggccttc agcgccaggg ccagattggt
     5581 cagcgggcca acggcgacca gggtcacctc accgggatgc tggcgggcga tgtcgatgat
     5641 cgcctgatag gccggcttgg cctcgggctg ggccgggacc agacccgtca gttccacatc
     5701 gcccaggccg ttctcgccat gcacgaaggt gggcgagggg ttgcgcgggc gggtcagggg
     5761 cttgtcggtt cccttataga ccggcgctga caggccaaag cgctgcttca gatagagcgc
     5821 gttgcgggtg gtggtttcga tgtcggcatt gccgaagatc gtggtgatgg ccagcaggtc
     5881 cagcgccggg ctggcctcga tgaacagcag ggccatggcg tcgtcgatgc cgggatcggt
     5941 gtcgaaaatg atcttttgcg tcatgtgcgg gctctagcgg ctttctgtcg ttggcgcgag
     6001 gcgttggtcg gccccggcct gcaggatcga gacgnnnnnn nnnngacctt ccagccgtcg
     6061 ccggtgcgga tccaggtctg gctctgacgg ccgatcttgt cagagccgtc gcgctggaac
     6121 gcgacattgg cgatggcgaa gtcggcgccg aaggtggtga tttcggtcct gagccgggtg
     6181 cggggcggcg agccgccggc ccggccgacg cggaaggcgg cgatctggtc aaagccccag
     6241 aggttttcgg tgacgcccat gcgcacggtg tgcggcgagt tccagaacgc gccgtccaga
     6301 gcctcgacgt cgttgtccat cagcgcggtc tcataggcct cgacggcggc ggtcacctcg
     6361 gccagtgcgg cgggatcatt gacgatcaca gcgggcctcc cggcgcatga gccg
//
LOCUS       DAOMEO010000027         6270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1711_length_6270_cov_2.749155, whole genome shotgun sequence.
ACCESSION   DAOMEO010000027 DAOMEO010000000
VERSION     DAOMEO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1711_length_6270_cov_2.749155"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..651)
                     /locus_tag="PLH31_01185"
     CDS             complement(178..651)
                     /locus_tag="PLH31_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheE"
                     /protein_id="HQR87895.1"
                     /translation="MSVVRKFRPPNRLAKLIKDRGGLMAKDAIAAAEVGVESLREASL
                     AVLDEALSEIERRFGPGAPDRESESFESLYGLTLQMIDVSTFVADAGVDQAAISLAAI
                     TDSCAEAGVWRWNAIDVHLNALRLLRTVGAQLPAADRQAMLEGLYKVSHRKIDEL"
     gene            complement(648..1247)
                     /locus_tag="PLH31_01190"
     CDS             complement(648..1247)
                     /locus_tag="PLH31_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR87896.1"
                     /translation="MDVLQPSTRINLEKASVLVLDDNGPSLDILSQVVSGFGVNQLSR
                     AESVKEAQALVATKTFDLIISDVQIPEVDGIEFINWLRREAPETNRYVPVILVTGHTR
                     TTDIFRTRDAGANFTVAKPITPKVLLERIFWVAREERAFIECDTYAGPDRRFKYEGPP
                     IGVEGRRRDDLPAEVGTAQEANMSQDEINSLMKVAKVQI"
     gene            complement(1257..1643)
                     /locus_tag="PLH31_01195"
     CDS             complement(1257..1643)
                     /locus_tag="PLH31_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheU"
                     /protein_id="HQR87897.1"
                     /translation="MTAMPLSTSSIVTPEHLTPVGEFSRRLAAELMSAAAVCRDCEHI
                     AGDLAQGGAALESISRLQALDELSQRLHGLSDVLQRIGEHASGEWSLSIEPLLEGLGL
                     ADLVVRLRAAGEPEAWGVEAGELDFF"
     gene            complement(1649..2215)
                     /locus_tag="PLH31_01200"
     CDS             complement(1649..2215)
                     /locus_tag="PLH31_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515822.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HQR87898.1"
                     /translation="MTTGHPHDDLERSIKVHVTQGESHVSSDPNVVMTTVLGSCIAAC
                     IRDPQAGVGGMNHFLLPDAGDGRKDGDAVRYGAYAMELLINGLLKRGARRERMEAKIF
                     GGGKLFEGLSDVGASNASFAERFLRDEGIPIVSSSTGGVSARRVEFWPASGRVRQRLV
                     AVDNAPSDVRRPTPAPMPVQSGGDLELF"
     gene            complement(2212..2601)
                     /locus_tag="PLH31_01205"
     CDS             complement(2212..2601)
                     /locus_tag="PLH31_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR87899.1"
                     /translation="MPQASTISVLVVDDQLTMRALIRNALQQIGFKDIREAPDGEEAL
                     KNLLAKPANLVISDFNMPKMDGLALLRAVRSHPPIRQTAFVMLTGRADRELVQRAVQF
                     GVNNYCVKPFTVQGLKEKIEQVFGQLT"
     gene            complement(2606..3633)
                     /locus_tag="PLH31_01210"
                     /pseudo
     CDS             complement(2606..3633)
                     /locus_tag="PLH31_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
     gene            complement(3633..4511)
                     /locus_tag="PLH31_01215"
     CDS             complement(3633..4511)
                     /locus_tag="PLH31_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQR87900.1"
                     /translation="MTSASQTERREAIVDGEFAFTNTDFKKIAALLYAQAGISLPETK
                     ATLVYSRLAKRLRALGMKTFTEYCAFVALESSVDEQQEMLRALTTNVTRFFREPHHFD
                     DLRANILEPMADKVRAGGRLRLWSAASSSGQEPYSMAFTVLSVWPNAADLDIRILGTD
                     IDTNVLATGRAAVYDESLLEGIPAHVRNQYFERDPSDRRAYRVCEAARSLVAFRELNL
                     NGPSWPMKGPFDAIFCRNVVIYFDEPTQERVWNRFAPLLAPGGKLYVGHSERVGSSIT
                     AFESAGLTAYRKVGGR"
     gene            complement(4511..4978)
                     /locus_tag="PLH31_01220"
     CDS             complement(4511..4978)
                     /locus_tag="PLH31_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR87901.1"
                     /translation="MTDPATAPSTERRELISFRVGDQEYCVNIMAVREIRGWSPATVL
                     PQTPGYMRGVINLRGAVLPIMDLACRLGMPVIEPSVRSVFIVVQAGDRTVGLLVDAVS
                     DILSINDDMIQPTPDVACDTVRSFVRGIISIEDRMISEISLDRILPEREALAA"
     gene            complement(4982..>6270)
                     /locus_tag="PLH31_01225"
     CDS             complement(4982..>6270)
                     /locus_tag="PLH31_01225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQR87902.1"
                     /translation="RKGRFLTLTDDMVTQVQVQVDVPGPGQSVIRVDPERIDSLIDLV
                     GELVINQAMLAQRVGEYGIAPSSSLAMGLDELEQLTRQIQDSVMAIRAQPVKSVFQRM
                     PRLVREVANMTGKQARLVMDGENTEVDKTVIERLADPITHMLRNAIDHGLESPEERRA
                     AGKNPEGVVRLAALHRSGRIVIEVQDDGKGINRERVFSIAVKKGLIAPDLQLTDEEID
                     NLIFLPGFSTADKISDVSGRGVGMDVVRRSVQALGGRISITSRPGLGSTFTLSLPLTL
                     AVLDGMVVDVAGETLVVPLAAIVESLRPKAEEVRPLGPVGSVLAVRDSFVPLIDVGLT
                     LGYRTTSPSPTEGVVLLVEGEDGSRAALVADAIHGQRQVVIKSLEQNYQQVEGVAAAT
                     ILGDGRVALILDVDAVINLRRRETPRPVEPTLIAAE"
BASE COUNT         1120 a         1996 c         2082 g         1072 t
ORIGIN      
        1 gtcagataca gacaaacaag cgtccttgat tttgacaccg gtagcaaaga gctaacaaac
       61 cgtcgttatg acgacaaggc aagattgtga cggacgacag gagaagtcgt actcgtggct
      121 tttccgacac atcggttcgg atttcgaaga gccgtcaccg gccggagata gcgacggcta
      181 aagctcgtcg atcttgcggt ggctgacctt gtagaggccc tcaagcatgg cctggcgatc
      241 tgcggcaggg agctgggcgc ccacagtgcg cagcaggcgc agggcgttca ggtgcacgtc
      301 gatggcgttc cagcgccaga cgccggcttc cgcgcaactg tcggtgatcg ccgccaggga
      361 gatggccgcc tggtccacgc ccgcatcggc gacgaaggtg ctcacgtcga tcatttgcag
      421 ggtgaggcca tagagcgact cgaagctctc agactcgcga tcaggagcgc cgggcccgaa
      481 ccgacgttcg atttcggaca aagcctcgtc cagaaccgcc agcgaggcct cgcgcagact
      541 ttccaccccc acctcggcgg cggcgatggc gtccttggcc atcaggccac cacgatcctt
      601 gatcagcttg gccaggcggt tgggcgggcg gaatttgcga acgacgctca tatctgcacc
      661 ttcgccactt tcatcagact gttgatttcg tcctgagaca tattggcttc ctgggcggtt
      721 ccgacctcgg ctggcaggtc gtcgcgacgt cgcccctcga cgccaatggg cggtccctca
      781 tacttgaagc gacggtcagg gcccgcatag gtgtcgcact cgatgaaggc gcgttcttcg
      841 cgcgccaccc agaaaatccg ctccagcaga actttgggcg tgatcggctt ggccaccgtg
      901 aaattggccc ccgcgtcccg cgtccggaag atatcggtcg tgcgggtgtg gcccgtgacc
      961 agtatcaccg ggacatagcg attggtctcc ggcgcctcgc ggcgcagcca gttgatgaat
     1021 tcaatgccgt cgacctcggg aatctgaacg tcgctgatga tcaggtcgaa ggtcttggtt
     1081 gcgaccaggg cctgggcctc cttgaccgac tcggcacgac tgagctggtt aacgccaaaa
     1141 cccgagacca cctgcgacag gatatcgagt gaaggtccat tgtcgtccag caccagaacg
     1201 ctggccttct ccagattgat ccgtgtcgaa ggctggagga catccacgga cagggcctag
     1261 aaaaagtcga gttcgcccgc ctcgacgccc caggcctccg gttcaccggc ggcgcgcaac
     1321 cgcaccacca gatcagccag acccagacct tcgaggaggg gttcgatcga gaggctccat
     1381 tcaccggacg cgtgctcgcc aatgcgctgc agaacatcgg agagaccgtg cagacgctgg
     1441 ctcagctcgt ccagcgcctg gaggcgggag atgctctcca gcgccgcgcc gccctgggcc
     1501 agatcgccgg cgatatgttc gcagtcccgg cataccgccg cagccgacat cagctcggcc
     1561 gcgaggcggc gcgagaattc gcccaccgga gtcaaatgct cgggtgtgac gatcgaagat
     1621 gtcgaaagcg gcatggcggt catgtggatc agaaaagctc caggtcgccg ccggactgga
     1681 ccggcatcgg tgcgggggta ggacgacgga cgtcgctcgg cgcattgtcg accgccacca
     1741 ggcgctgacg cacgcggcca gaggccggcc agaactccac gcgacgggct gagacgccgc
     1801 cggtgctcga cgagacaatt ggaatgcctt cgtcgcgaag gaaacgctcg gcgaagctgg
     1861 cgttgctggc cccgacgtcg gacaggccct cgaacagctt gccgccgccg aagatcttgg
     1921 cctccatccg ctcgcgacgg gcgccgcgct tcagcaggcc gttgatcagc agctccatgg
     1981 cgtaggcgcc atagcggacg gcgtcgccat ccttgcggcc gtcgcccgca tcgggaagca
     2041 ggaagtggtt catgccgccg acgccagcct gcggatcacg gatgcaggcg gcgatgcacg
     2101 agcccagaac cgtggtcatc accacgttgg ggtcggacga cacgtggctc tcgccctgcg
     2161 tgacgtgaac cttgatcgag cgttccagat cgtcgtgtgg gtgaccagtc gtcatgtgag
     2221 ctgcccgaag acctgttcga tcttctcctt gaggccctga acggtaaacg gcttgacgca
     2281 ataattgttg acgccgaact gcacggcccg ttgcacgagc tcgcgatcgg cgcggccggt
     2341 cagcatgacg aaggctgtct gccggatcgg cgggtgcgac cgcaccgcgc gcagcagggc
     2401 cagaccatcc atcttcggca tgttgaaatc cgagatgacc aggttggccg gcttggccag
     2461 gaggttcttc agagcctcct cgccatcggg cgcctcccgg atgtccttga acccgatctg
     2521 ctgcaacgca ttgcggatca aggcccgcat ggtcagctga tcatcgacga cgagaacgga
     2581 aattgtgctt gcttggggca tgggttcatc gcctcgcgga ggccagatcg aggatggatt
     2641 ggcccatgga ggacagggaa acctgcttct ccaccgcgcc gatttcaaag gccgaggggg
     2701 catgccgtaa acgacacagc tttgctcgtc ctgaccgagg gtttttgcgc cggccttgcg
     2761 catcgtcaga aggccctggg ctccgtcacg gcccatgccg gtcaggatga cgccaacagc
     2821 cttgtcgccg accgcattgg ccaccgagtt gaacagcacg tccaccgatg gacggtggcc
     2881 gctgaccgga tcgccctgca ccaggcggca gcgcagaccg gccgaacgaa cgacctcaag
     2941 gtgggtcgcg ccgcctgggg cgacatagac cttgcccggc tccagcatcg cgccgtcagt
     3001 agcttcctgc accttggcgc cgctggcgcg atcgagcctt gcagcgaagc tggcggtaaa
     3061 ggtcgccggc atgtgctggg tgatgaccgt aggcggacag gtgtccggga acatcgtcag
     3121 aatggccagc agcgcctcga cgccgccggt cgacgatccg atcgcgacga tgtcgcccga
     3181 cggcatgaag gtcttggtcc gcgcggcggc gggggcggtg tcggccttgg tccgcaccga
     3241 ggcgcgggcc gcgatcttga ccttgaccac gatctcgttg agcgcgtcct gggtgcccag
     3301 ggcgtcggcg ggcttggcca cgcagtcgat ggcgccaagc tccagggctc gtagcgtcat
     3361 ctcggcgccg gcctgggtca gggtcgagac catcaccacc ggcatcggtc gcaggcgcat
     3421 gatcttctcg aggaactcga tgccgttcat gttcggcatc tcgatgtcga gggtgacgac
     3481 gtcgggattg agggccttga tcatgccccg ggcctcgaag gggtcgccgg cggcgccgca
     3541 gacctcgata tccggatccc gattgagggc tgccgtgatc aggctccgca tggtggcgga
     3601 gtcatcgacg acgagaacgc gaatcttggc catcagcgac ctcccacctt gcgataggcc
     3661 gtcaggcccg cgctttcaaa ggcggtgatc gagctgccga cccgctcgga gtggcccaca
     3721 tagagcttgc cgcccggcgc cagtagcggg gcgaaacggt tccagacgcg ctcctgggtc
     3781 ggctcgtcga aatagatgac gacgttgcgg cagaagatgg cgtcaaacgg acccttcatc
     3841 ggccagcttg ggccattgag gttcagttca cggaaggcca ccaggctgcg cgcggcctcg
     3901 cagacgcggt aggcgcgtcg atcactggga tcgcgttcga aatactggtt gcgaacgtga
     3961 gccggaatgc cttccagcag gctctcgtca tagacagcgg cgcggccggt ggcgagcacg
     4021 ttggtgtcga tgtctgtgcc caggatccgg atgtcgagat ccgccgcatt gggccacacc
     4081 gacagcaccg tgaaggccat cgaatagggc tcctggcccg acgagctggc cgccgaccac
     4141 aggcgcagac ggcccccggc gcgaaccttg tcagccatcg gctcgaggat attggcgcgc
     4201 aggtcgtcga agtggtgcgg ttcgcggaag aagcgggtga cgttggtcgt cagggcgcgc
     4261 agcatttcct gctgctcgtc cacactgctc tccagcgcca cgaaggcgca gtactcggtg
     4321 aaggtcttca tgcccagggc gcggagacgc ttggccagac gggaatagac cagcgtcgcc
     4381 ttggtctcgg gcaggctgat gccagcctgc gcatagagca gcgccgcgat cttcttgaag
     4441 tcggtgttcg tgaacgcgaa ctcgccatcg acgatggcct cgcgacgttc tgtttgggag
     4501 gctgaggtca tcaggcggcc agggcctcgc gttcggggag gatacggtcg agcgagatct
     4561 cgctgatcat gcggtcctcg atcgaaatga tgccgcggac gaaactgcga accgtgtcgc
     4621 aggccacgtc gggcgtaggc tgaatcatgt cgtcgttgat cgacaggatg tccgacacgg
     4681 cgtcgaccag caggccgaca gtgcggtcgc ccgcctgaac aacgataaac acactccgta
     4741 cgctgggctc gatcaccggc atgcccagac ggcaggccag gtccatgatc ggcaacaccg
     4801 cgccgcgcag attgatcacg ccgcgcatgt agccgggcgt ctgcggcagc acggtcgccg
     4861 ggctccagcc gcggatctcg cgcacggcca tgatgttcac gcagtattcc tggtctccga
     4921 cccggaagga aataagctcc ctgcgttcag tgctcggggc ggtagctggg tcagtcatga
     4981 cttactccgc tgcaatgagg gtgggttcga cgggacgggg cgtctcccgg cggcgaagat
     5041 tgatcaccgc gtcgacgtcc aggatgaggg ccacacggcc atcgcccagg atcgtcgcgg
     5101 cggcgacgcc ttcgacctgc tggtagttct gctccagcga cttgatgacg acctggcgct
     5161 gaccgtggat ggcgtcagcc accagggcgg cgcgcgagcc gtcctcgccc tcgaccagca
     5221 gcaccacgcc ttcggtcggc gacggcgagg tggtgcgata gcccagggtc aggccgacat
     5281 cgatcagcgg cacgaagctg tcgcggaccg ccagcaccga gccgacgggg cccaaggggc
     5341 gaacctcttc ggccttcggg cgcaggcttt cgacgatagc ggccagcggc acgaccaggg
     5401 tctcgccggc cacgtcgacc accatgccgt ccagaacggc cagagtgagg ggcaggctga
     5461 gcgtgaaggt cgagcccagg ccaggacgcg aggtgatcga gatgcgaccg cccagagcct
     5521 ggacgctccg gcgcaccacg tccatgccga cgccgcggcc cgacacgtcc gagatcttgt
     5581 cggcggtgga gaagcccggc aggaagatca gattgtcgat ctcctcgtcg gtcagctgca
     5641 ggtcgggcgc gatcaggccc ttcttgacgg cgatcgagaa gacccgttcg cgattgatac
     5701 ccttgccgtc atcctggacc tcgatgacga tccggccgct gcggtgcagg gcggccaggc
     5761 gcacgacgcc ctctggattc ttgccggcgg cgcgtcgctc ttcggggctt tccagcccgt
     5821 ggtcgatggc gttgcgcagc atgtgggtga tcgggtcggc cagccgttcg atgaccgtct
     5881 tgtcgacctc ggtattttcg ccgtccatca ccaggcgggc ctgcttgccc gtcatgttgg
     5941 cgacttcgcg aaccagacgc ggcatgcgct ggaacaccga cttcaccggc tgggcgcgga
     6001 tggccatcac gctgtcctgg atctggcggg tgagctgttc cagctcatcc agacccatgg
     6061 ccaggctgga ggacggcgcg atgccgtact cgccgacccg ctgggccagc atcgcctgat
     6121 tgatcaccag ttcgccgacc aggtcgatca ggctgtcgat acgctcggga tcgacgcgga
     6181 tcaccgactg gccggggccg ggcacgtcaa cctggacctg gacctgggtc accatgtcat
     6241 cggtgagcgt gaggaaccgg cccttgcgga
//
LOCUS       DAOMEO010000028         6191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1734_length_6191_cov_2.770209, whole genome shotgun sequence.
ACCESSION   DAOMEO010000028 DAOMEO010000000
VERSION     DAOMEO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_6191_cov_2.770209"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLH31_01230"
     CDS             <1..213
                     /locus_tag="PLH31_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HQR87903.1"
                     /translation="NAPGGERLKFEGTVAGRRIPTPEEIAEINKSLEKLDGAAEDKSA
                     AVEAPKPPPPPPKPRARPPSLFNPLN"
     gene            complement(295..>408)
                     /locus_tag="PLH31_01235"
     CDS             complement(295..>408)
                     /locus_tag="PLH31_01235"
                     /inference="COORDINATES: protein motif:HMM:NF022199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR87904.1"
                     /translation="AVIQGLSTLARDGDAREKLTRVAEMAMAAWPEAGRPG"
     assembly_gap    409..418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<419..926)
                     /locus_tag="PLH31_01240"
     CDS             complement(<419..926)
                     /locus_tag="PLH31_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR87905.1"
                     /translation="MNHADTKPSRRIGRPLSFDRDAALHQAMLLFWRYGYEGTSLSDL
                     TAAMGVTAPSIYAAFGDKRRLFLEAVDRYLSGPVTAKSIIAGAATAREAAAGLLHASV
                     AGFTGADTPPGCLLASATISCSAAAMDIQQALAARRGGIEADLRVRILKAMETGELPA
                     RTDADALAG"
     assembly_gap    1021..1030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1031..2131
                     /locus_tag="PLH31_01245"
     CDS             <1031..2131
                     /locus_tag="PLH31_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR87906.1"
                     /translation="DYRTLGRSGLAVSPLALGTMTFGTARWGTGQAGSKAVFDAYVDA
                     GGNFVDTADVYSGGRSEEMLGDFIAERGLRDQLVVATKAGFSAGQGPHAGGLGAKHLH
                     TALEGSLRRLRTDHVDLFWTHVWDAVTPAEELLETMAGLIRSGKIRYWGMSNAPAWYV
                     AKVATLATVRGLPGPIALQYFYSLANRDIEDEHLPLAAEFGLGVLPWSPLAYGLLTGK
                     YDRATVEASAPRAGGLPRDAALVAEQRPDDDKRLDGANPFGDTLFTDRNWAIVDAVAQ
                     VARETGHSPASIALAWVTGRRGVTSTLMGVSRVQQAQDNIAALAIELSDEHRATLDAV
                     SAPDGRMLYSLFLPAMRRHAVSGGSLVRAWRD"
     gene            complement(2145..3155)
                     /gene="trxB"
                     /locus_tag="PLH31_01250"
     CDS             complement(2145..3155)
                     /gene="trxB"
                     /locus_tag="PLH31_01250"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQR87907.1"
                     /translation="MSTSTAPRTTRCLIIGSGPAGYTAAIYAARALLKPVLIAGIQPG
                     GQLTITTDVENYPGFADVIQGPWLMDQMKAQAEHVGTEFVNDIVLSADLSKRPFHIKC
                     DSGEEWLAETLIIATGAQAKWLGLESESKFQGFGVSACATCDGFFYRNKEVVVVGGGN
                     TAVEEALFLTNFASKVTLVHRKDELRAEKILQERLLAHPKIELAWNSAIDEIVGDTNP
                     MGVTGVRLKNVVTGDLTELKCDGVFIAIGHAPSSELFKGQLDTHANGYLTVKPGTTST
                     TIPGVYAAGDVTDDIYRQAVTAAGMGCMAALEAVRFLAEEDHAKAHHPISHAEAEKIG
                     VW"
     gene            3214..3489
                     /locus_tag="PLH31_01255"
     CDS             3214..3489
                     /locus_tag="PLH31_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87908.1"
                     /translation="MKAGLPVGMGAGIAIGVALGLALDNLALGIGLGVAIGAGSGVAM
                     MGSGAAKEKKKDGGDGGAHGGGASDTSSDCGSSDGGSCGDGGGGGGD"
     gene            complement(3515..3739)
                     /locus_tag="PLH31_01260"
     CDS             complement(3515..3739)
                     /locus_tag="PLH31_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQR87909.1"
                     /translation="MPNADYRVVVEPIPAEDGGGFLAVVPDLPGCMSDGETQAEALQN
                     VQKAIIDWIGHAQSEGQTVPPPSRHFHYGV"
     gene            4151..>5227
                     /gene="pseI"
                     /locus_tag="PLH31_01265"
     CDS             4151..>5227
                     /gene="pseI"
                     /locus_tag="PLH31_01265"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HQR87910.1"
                     /translation="MTASPSITIAGRQIGADHSPYVICELSGNHNGSLERCLTMVDAA
                     AATGCDAIKIQTYTADTITLDVDRPEFRIHGGLWDGRTLYELYQEAQTPFEWHAAIFE
                     RARQHGVTLFSSPFDETAVDLLDGLGAPAFKIASFEAVDLPLIKYAASKGKPLIISTG
                     MANLQEMQTALDTALGAGADGVMLLHCVSSYPSTFADANVRTVADMAQRFGCPVGLSD
                     HTPGTAASVAAVSVGACAVEKHFTLARSDGGPDAAFSLEPVEFKALVDDCRNAWAALG
                     RAHYDTLGSEQASLLFRRSLYVTADVKAGEPLTRANVRSVRPAGYSLRRSCPCALIEG
                     AYSAAAAMARSARRSRAVRRSVSLL"
     assembly_gap    5228..5237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5238..5350
                     /locus_tag="PLH31_01270"
     CDS             <5238..5350
                     /locus_tag="PLH31_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HQR87911.1"
                     /translation="VRPGNGLPPADLDKVLAGKASRDLTRGEPLDWSMVG"
     gene            complement(5361..5750)
                     /locus_tag="PLH31_01275"
     CDS             complement(5361..5750)
                     /locus_tag="PLH31_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HQR87912.1"
                     /translation="MTAPCQSPVEIVRLFLKDMERLDYDSALKWVSDSCEYVNPPPLP
                     VVHGPAGIRGVLEPFFAPTLENEFKLLREAVSGPLVFLERLDRHRLADKWVELPVTGV
                     FEVHDGKITYWRDYFDAATILSQWPAG"
     gene            complement(5843..>6191)
                     /locus_tag="PLH31_01280"
     CDS             complement(5843..>6191)
                     /locus_tag="PLH31_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HQR87913.1"
                     /translation="HVDTQDMPRLTLAADLAIGAGGSSSWERCILALPTLTLVLADNQ
                     VAVAAALTEAGASLRLDASSADFDVAFGQQVDRLVADGALRAMLSAASALVCDGDGAR
                     RVAAAFLAHISRT"
BASE COUNT          991 a         2011 c         2122 g         1037 t
ORIGIN      
        1 aatgcgccgg gcggcgagcg cctgaagttc gagggcacgg tcgccggccg ccgcatcccg
       61 acgcccgaag agatcgccga gatcaacaag tccctggaaa agctggacgg cgcggccgag
      121 gacaagtcgg cagcggtcga ggccccgaag ccgccgccgc cgccgcccaa gccgagggcg
      181 cggccgccga gcctgttcaa tccgttgaac tagggcgagt tccggctcga ccggatcctg
      241 gattgcagac tgtaatccac ggcgggcttg aacgatcccg cgctgacccg ctgctcagcc
      301 cgggcgccct gcctccggcc aggcagccat ggccatttcg gccacgcgcg tcagcttctc
      361 ccgcgcgtcg ccgtcccggg ccagggtgga caggccctgg ataaccgcnn nnnnnnnngt
      421 ccggccaggg cgtcggcgtc cgttcgcgcg ggcagctcgc cggtctccat ggctttgagg
      481 atccggacgc ggagatcggc ttctatacct cccctgcgcg ccgcgagggc ctgctgaatg
      541 tccatcgcgg cggctgaaca actgatcgtg gcgctggcca acaggcaccc gggtggagtg
      601 tcggcccccg tgaacccggc caccgaggcg tggagcagac ccgcagcggc ctcgcgtgcg
      661 gtggcggcgc ccgcgatgat cgatttcgcg gtcacggggc ctgagaggta gcgatcgacg
      721 gcctccagga acagccgtcg cttgtctccg aacgccgcat agatgctggg cgccgtcacc
      781 cccatggcgg cggtcaggtc gctcagcgag gtcccctcat agccgtaccg ccagaacagc
      841 agcatggcct gatgcagcgc ggcgtcgcga tcgaacgata ggggccggcc gatcctgcgt
      901 gagggtttgg tgtccgcatg attcatagcg atcaatatga aacccttgcg acggcgcgac
      961 aagcggtgat aggcattcca tatcgatcac taggaaattt tgacatgggc ctctccgatt
     1021 nnnnnnnnnn gattaccgca cgctgggccg ttcgggtctg gccgtcagcc cgctggcgct
     1081 ggggacgatg acgttcggca cggcgcgctg gggcacgggc caggcggggt cgaaggccgt
     1141 gttcgacgcc tatgtcgacg ccggcggcaa tttcgtggat acggcggacg tctattcggg
     1201 cggtcgcagc gaggaaatgc tgggcgactt catcgccgag cgcgggctgc gcgatcagct
     1261 tgtcgtggcg accaaggccg gcttctcggc agggcagggg ccccatgccg gcggcctggg
     1321 cgccaagcac ctgcacacgg ccctggaagg ctcgcttcgg cgcctgcgca ccgaccatgt
     1381 cgacctgttc tggacccatg tctgggacgc tgtcaccccg gccgaggaac tgctggaaac
     1441 gatggccggg ctgatccgct cgggcaagat ccgctattgg ggcatgtcga acgcgccggc
     1501 ctggtatgtc gccaaggtcg ctaccctggc caccgtgcgc ggactgccgg ggccgattgc
     1561 gctgcagtat ttctattccc tggccaaccg cgacatcgag gacgagcatc tgcccctggc
     1621 cgctgagttc ggcctgggcg tgctgccctg gagccccttg gcttacgggc ttctgaccgg
     1681 caaatatgat cgcgcgacgg tcgaggcctc ggccccccgg gcgggcggac tgccccgcga
     1741 cgcggcctta gtcgccgagc aacgcccgga cgacgacaag cgcctggatg gcgccaatcc
     1801 gttcggcgac acgctattta ccgaccgcaa ctgggctatt gtcgatgcgg tggcgcaggt
     1861 ggcccgggaa accggccaca gccccgccag tatcgccctg gcctgggtga ccggacggcg
     1921 cggggtcaca tccaccttga tgggcgtcag ccgcgttcag caggcgcagg acaacatcgc
     1981 cgccctggct atcgagctgt cggacgaaca ccgcgccacg ctcgacgccg tcagcgcgcc
     2041 agacggccgc atgctctaca gcctgttcct gcctgcgatg cggcggcatg ccgtgtcggg
     2101 tggaagcctg gttcgggctt ggcgcgactg acggcaggcc agccctacca gacgccgatc
     2161 ttttcggcct cggcgtggct gatcgggtgg tgagccttgg cgtggtcttc ctcggccagg
     2221 aaacgcacgg cctccagggc ggccatgcag cccatgccgg cggcggtgac ggcctggcgg
     2281 tagatgtcgt cagtgacgtc gccggccgca taaacgccgg ggattgtggt cgaggtggtg
     2341 ccgggcttga cggtcagata gccgttggcg tgggtgtcca gctggccctt gaacagctct
     2401 gaagacgggg catggccgat ggcgatgaac acgccgtcgc atttcagctc ggtcaggtcg
     2461 ccggtgacca cgttcttgag gcgaacgccc gtcacgccca tcggattggt gtcaccgacg
     2521 atctcgtcga tggcgctgtt ccaggcgagt tcgatcttcg ggtgggccag caggcgctcc
     2581 tgcaggatct tctcggctcg cagctcgtcc ttgcggtgga ccagggtgac cttgctggcg
     2641 aagttggtca ggaacagggc ttcctcgacg gccgtgttgc cgccgccgac caccacgacc
     2701 tccttgttgc gatagaagaa gccgtcgcag gtggcgcagg cgctgacgcc aaagccctgg
     2761 aacttgctct cgctctccag gcccagccac ttggcctggg cgccggtggc gatgataagg
     2821 gtctcggcca gccactcctc gccgctgtcg cacttgatgt ggaacgggcg cttggacagg
     2881 tcggcgctca gcacgatgtc gttgacgaac tcggtgccca catgctcggc ctgggccttc
     2941 atctggtcca tcagccaggg gccctggatc acgtcggcga agcccggata gttctcgaca
     3001 tcggtcgtga tggtcagctg gccgcccggc tggatgccgg cgatcagcac cggcttgagc
     3061 agcgcgcgcg cggcatagat ggcggcggta tagccggcgg ggcctgagcc aatgatcagg
     3121 cagcgggtgg tgcggggggc ggtggaggtc gacatgagcg cagatatagt gtcgattccg
     3181 atttagtgcg atggccgcag caagggggaa atcatgaaag cgggtttgcc tgtcgggatg
     3241 ggcgccggga tcgccatcgg cgtggcgctg ggcttggcgc tggacaatct ggccctcggt
     3301 atcggccttg gcgtcgccat cggcgcgggc tcgggcgtgg cgatgatggg gtcgggcgcg
     3361 gccaaggaaa agaagaagga tggcggcgac ggcggagcgc acggcggcgg cgcgtcggac
     3421 acatccagcg actgcggcag ctctgacggc gggagctgtg gagatggcgg cggcggtggc
     3481 ggggattgat gccgcggcct atgccgcctg aaactcagac gccgtagtga aaatgccggc
     3541 tgggcggcgg cacggtctgg ccttctgact gtgcgtgtcc gatccagtcg atgatcgcct
     3601 tctggacatt ttgcaaagcc tcggcctgcg tttcgccgtc cgacatgcag cccggaaggt
     3661 cggggacaac cgccagaaag ccgccgccgt cttcagcggg gatgggttcg acaacgacgc
     3721 gatagtcagc gttaggcatc gtttgcgggt cctattgcct gtctaaggta ggccacgagg
     3781 tgttttatgt acaccggctt gatcgggcga cgggcgggaa ccgtaaggat tccaacgacc
     3841 aaggggtggg atatcgtata gtgcgaaccg ccgcccgatg gcggttcgca ggttaggccg
     3901 aagtagcggc agacaacttc gacatcggcg atgcgccaat ccccaacggg atttcgcgac
     3961 atggcgacga acagcttttc tccttgtgac aactcgccct cgcctgctga gaagcgattt
     4021 acccacaagg ttgagcttct tgtaaattgc tcaacctata atacttgttc agaagcagcg
     4081 gcggcgattg actcatcgtc cacctttccc gggttaaact ggcggaaacc atgttgcccc
     4141 ggaccgcgcc atgaccgcct ccccctccat caccatcgct ggccgccaga tcggcgctga
     4201 ccactcgccc tatgtcatct gcgagctgtc gggcaatcac aacggtagcc ttgagcgctg
     4261 cctgaccatg gtggacgccg ccgccgcgac cgggtgcgat gcgatcaaga tccagaccta
     4321 taccgccgac accatcacgc tggatgtcga ccggcccgag ttcaggatcc acggcgggct
     4381 gtgggacggt cgcacgctgt atgagctgta ccaggaagcc cagaccccgt tcgaatggca
     4441 cgcggcgatc ttcgagcgcg cccgccagca cggcgtgacc ctcttctcca gcccgttcga
     4501 cgagacggcc gtcgatctgc tcgacggcct gggcgcgccg gccttcaaga tcgcctcctt
     4561 cgaagccgtc gacctgccgc tgatcaagta tgccgccagc aagggcaagc cgctgatcat
     4621 ctcgaccggc atggccaatc tgcaggagat gcagaccgcg ctcgacaccg ccctgggcgc
     4681 cggggccgac ggggtgatgc tgctgcactg cgtgtccagc tacccctcca ccttcgccga
     4741 cgccaatgtg cgcacggtgg ccgacatggc ccagcgtttc ggctgcccgg tggggctgtc
     4801 ggaccacacg ccgggaaccg cggcctcggt ggcggcggtt tcggtcggcg cctgcgcggt
     4861 ggaaaagcac ttcaccctgg cccgctctga tggggggccg gatgcggctt tcagcctgga
     4921 gccggtcgag ttcaaggccc tggtcgacga ttgcaggaac gcctgggccg cgctcggccg
     4981 cgcccactac gacacgctgg gttccgagca ggccagcctg ctcttccgcc gctcgctcta
     5041 tgtcaccgcc gacgtcaagg ccggcgagcc cctgacccgc gccaatgtcc gctcggtccg
     5101 cccggcggga tatagcctgc ggcggtcttg cccctgcgcc ctcatcgaag gcgcctattc
     5161 cgccgccgcc gccatggcgc gcagcgccag gcgctcgcgg gccgtgaggc gctcggtttc
     5221 gctcttgnnn nnnnnnncgg tccgccccgg caacggcctg ccgccggccg atttggacaa
     5281 ggtgctggcc ggcaaggcct cgcgagacct gacgcgcggc gagccgctgg actggtcgat
     5341 ggtggggtag gcgccggcgc ttatccggcg ggccactgcg agaggatggt cgccgcgtcg
     5401 aaatagtcgc gccaataggt gatcttgccg tcgtgaacct cgaacacgcc ggtcaccgga
     5461 agctcgaccc atttgtcggc gaggcgatga cggtccagcc gctccagaaa gaccaggggc
     5521 ccgctgacgg cctcgcgcaa cagcttgaac tcattctcca gggtcggggc gaagaaaggc
     5581 tccagaacgc cccggatacc ggccgggcca tgcaccacgg gcagcggcgg cgggttcaca
     5641 tattcgcagc tgtccgaaac ccacttcagg gctgaatcgt agtcgaggcg ctccatgtcc
     5701 ttcaggaaca ggcggacaat ctcgacgggg ctttggcagg gggcggtcat gagggctctc
     5761 cggtgtgcga tggctcatgg tctgcatctc tgggtgtgtc cgcaattacc tcacaggtca
     5821 tcgcagccgg cgctgtagcg cttcaggtcc gggatatgtg cgccagaaac gccgccgcca
     5881 cccggcgggc gccgtcgccg tcgcagacaa gggcgctggc cgccgacagc atcgcgcgca
     5941 aggctccgtc cgcgaccaag cggtcgacct gttgcccgaa cgcgacgtcg aaatctgccg
     6001 acgaggcgtc aaggcgaagg cttgcgcccg cctccgtcaa ggcggcggcg acggcgacct
     6061 ggttgtcggc cagaaccagg gtcagggtcg gcagggcgag gatgcaacgc tcccagctgg
     6121 atgagccgcc ggccccgatg gcgaggtcgg ccgccagggt caggcggggc atgtcctggg
     6181 tgtcgacatg c
//
LOCUS       DAOMEO010000029         6141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1746_length_6141_cov_2.464673, whole genome shotgun sequence.
ACCESSION   DAOMEO010000029 DAOMEO010000000
VERSION     DAOMEO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_6141_cov_2.464673"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PLH31_01285"
     CDS             complement(<1..1497)
                     /locus_tag="PLH31_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HQR87914.1"
                     /translation="MSNLVLSAPLKGWIAPLDETPDAVFAERMLGDGLAIDPTGSMLH
                     APCDAVVMTVHRARHAVTLRAANGAEILMHLGLETVALEGAGFEVQVREGQSVKAGDP
                     LIAFDLDFLARNVRSLMTPIVITNLDAFQIVRRQQDQLAEVGDVLMELSPIGGPAAPL
                     AVAGREIRREVVVPLVHGIHARPAARIAEIARRFAADVALVAVNRRASAKSPVGVMSL
                     AIRHGDTITIAASGLDADFAIAAIAELIEGGMGEGGPVAAPVAPAPTEPVIEAGPARL
                     PKDRVLKGVLAAPGLAIGKAVRLSSSEIIVREAGEGVAIEHAALTQALAKVRGRLETA
                     ASQESAKGGDKARKAILGAHLAFLEDPELSAGARALIDQGKSAGFAWRKAVGGYVDAL
                     RGLGDRRLAERVDDLVDLERQVLRALTGEEDSVAIMAAGSILLADELLPSQLMGVDPS
                     LVAGFCTARGGPTSHVAILAAAMGIPALVAVGGAVMSVADGTALILDAD"
     gene            complement(1518..3806)
                     /locus_tag="PLH31_01290"
     CDS             complement(1518..3806)
                     /locus_tag="PLH31_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQR87915.1"
                     /translation="MILVRSVAALASAIALVAAVPALADPVALTPMPAKLSTAEGVFP
                     LTAKTRIFVAKGDIEARGVAAQLSDLLFRARGLKPVVVEGAPSAGEAAIVLARGGATG
                     EGYALQSGPSGVTITAAKRAGLFYGAMTLWQLATQEAGKGPADLPSVAIEDAPRFGWR
                     GLMIDSARHVQSVETLRGIIDAMAVHKLNTFHWHLVDDQGWRLEIKKYPKLTEVGAWR
                     TPAGAAGRDPKTGKAIRYGGFYTQDEVRELVAYAAARNVTIVPEIEMPGHALAPIVAY
                     PHLGVNPNPPAIDRNNWGIFPWLYNLDDSTFAFLNDVLDEVMDLFPSDYIHVGGDEAV
                     KDQWKASPKIQAQIKALALKDEHEAQSYFIQRIGKHLDKRGRRLIGWDEILEGGLAPN
                     ATVMSWRGLEGAIAAAKLGHDTVLSPWPLMYLDNRQSASPDEPPGRGRVVSLKDVYDF
                     DPAPKEMTEVERKHVIGLQGNAWTEHMRTDVRLERMIFPRLVAVAERGWSPQTTTDWA
                     DFNRRMPAEFARLDALGVAYDAVPFEPQAKLSPGEGGKVDVALASSLAIGEIRYTVGG
                     AEPTTGSNLYEKPLALAANDKLRARTFVDGRPLGRVRDLSVNPDSLLTRTGHQLKACS
                     DKLTLLLEDDAPVQGDRAVFHVDIMNPCWIWEGADLAKGATLTARIGQAPFNFQIGAD
                     REKIPLRAPATPEGELEVLLGCEGERIAAIPLGKAVREPALSTISGKIPARPGKHDLC
                     LMFTSKSVDPTFAIDRVTLKSN"
     gene            complement(4023..>6141)
                     /locus_tag="PLH31_01295"
     CDS             complement(4023..>6141)
                     /locus_tag="PLH31_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87916.1"
                     /translation="ANASVQGLYATRAEKWTPQVSGMVSWKNEASTFGALVGAFYQER
                     EFRRDGQEILGYTEYQNFAGSGQTVLAPNLIGNAFFQQTRKRKGANFVTQWEPNEKLE
                     LSFSGLYTKMDADNTNVNRMAWVSNLITGSTGPAGSAGNALTAYKISNGILNSATRAA
                     AAPNGTKSRGMVQDDIFRTAHSTSWDLNLDGQYQATDNLKISGNIGYTKGEGATDDTE
                     AWETYWETGMSYELGAPVTSVSYPGLPAVGSAAYLNNFYSWSWGGKVESPDEEFYAKG
                     DVEYTVDNGVLKTLLFGGRYTDHTREVNYTAYAWAGNGVYSGTSGVDLGSVVGKSTPS
                     NYGEDIGLSGQGYVLADRGKVLAALAGNGGRQFAYYPPLSFAVQEKTQAVYAMAKLGG
                     DRWRGNVGVRAVHTDQDTTQYSASVTPATVTNVFGSFGPRNVNRDYWDILPSATLVVN
                     ADEDVVVRLSAAKVMSRPGYAQLAGAFSLNDTAVTGTAGGNPELDPFRAWQYNAAAEW
                     YYGPEALLSVGVFLLDIESYITSRTYQEFHITQQTQQRLGPGKGDTFTMTGPINGDGG
                     TNRGFEVTLQQPIGGGFGFIGNYTYSDAKTDLGKPIDGSSKHTYNLTGYFENDLLSAR
                     LAYSYRSKFKSGTDRGTDMFQDNFATLDGSVSVNLNKTIALTFDAQNLLNEKLKYFVG
                     TPDVPRAIYDNGRTFYAGVRFKF"
BASE COUNT         1004 a         1991 c         2111 g         1035 t
ORIGIN      
        1 gtcggcgtcg aggatcaggg cggtcccgtc ggccacgctc atcaccgcgc cgccgaccgc
       61 gaccagggcc ggaatgccca tcgcggcggc caggatcgcc acgtgcgagg tgggtccgcc
      121 gcgcgcggtg cagaacccgg cgaccagcga gggatcgacg cccatcagct gcgagggcag
      181 cagctcgtcg gccagcagga tcgatccggc cgccatgatc gcgacgctgt cctcctcgcc
      241 ggtcaaggcg cgcaggacct ggcgctcgag gtccacgagg tcgtcgaccc gctcggccag
      301 gcggcggtcg cccagaccac gcagcgcgtc gacatagccg ccgaccgcct tgcgccaggc
      361 aaagccggcg ctcttgccct gatcgatcag ggcgcgggct ccggccgaca gttcgggatc
      421 ttcgaggaag gccaggtggg cgcccaggat cgccttgcgc gccttgtcgc cccccttggc
      481 gctttcttgg gacgcggccg tctccagtcg gccgcgcacc ttggccagcg cttgcgtcaa
      541 ggcggcatgc tcgatcgcga cgccctcccc ggcctcgcgg acgatgatct cggaagacga
      601 cagtcgcacg gccttgccga tggccaggcc cggtgcggcc agcacgcctt tcagcacccg
      661 atccttgggc agcctggcgg gcccagcctc gatgaccggc tcggtcggcg cgggagcaac
      721 cggtgcggcg acgggaccgc cctcgcccat gcccccttcg atcagttcgg cgatcgccgc
      781 gatggcgaaa tccgcgtcga gaccgctggc ggcgatggtg atcgtgtcgc catggcggat
      841 cgccaaggac atcacaccca ccgggctctt ggcgctggcc cgacgattga ccgccaccag
      901 agccacgtcg gcggcgaagc ggcgcgcgat ctcggcgata cgggcggcgg gacgggcgtg
      961 gatgccgtgg accaggggca cgacgacctc acgcctgatc tcgcgccccg cgaccgccag
     1021 gggtgcggcg gggccgccga tcggtgacag ctccatcagg acatcgccga cctccgccag
     1081 ctgatcctgc tggcgacgca caatctggaa ggcgtccagg ttggtgatga caatcggcgt
     1141 catcaggctt cggacattcc gggccagaaa atcgagatcg aaagcgatca gcgggtcgcc
     1201 ggccttgacg ctctggccct cgcggacctg cacctcgaaa cccgccccct ccagcgccac
     1261 agtctccaga cccagatgca tgaggatctc ggcgccgttg gcggcccgca gggtaaccgc
     1321 gtggcgcgcc cgatgcaccg tcatcaccac cgcgtcgcaa ggcgcgtgca gcatcgagcc
     1381 cgtcgggtcg atggccagac cgtcacccag catgcgctcg gcgaacacag cgtcgggcgt
     1441 ctcgtcgagc ggcgcgatcc agcccttcag cggcgcggag agaacgagat tggacacggg
     1501 gctactctct cgaaaagcta gttcgacttg agcgtgacgc gatcgatggc gaaggtcggg
     1561 tcgacgctct tggaggtgaa catcaggcac aggtcgtgct tgcccgggcg cgcagggatc
     1621 ttgccgctga ttgtcgacag ggctggctcg cggacggcct tgcccagcgg gatggcggcg
     1681 atgcgctcac cctcgcagcc cagcagcacc tccagctcac cctcgggcgt agccggcgcg
     1741 cgcagcggaa tcttctcgcg atcggcgccg atctgaaagt tgaacggcgc ctggccgatg
     1801 cgggcggtca gggtcgcgcc cttcgccagg tctgccccct cccagatcca gcaggggttc
     1861 atgatatcga cgtggaacac ggcgcggtca ccctggaccg gggcgtcgtc ttccagcaac
     1921 agggtcagct tgtcgctgca cgctttcagc tggtggcctg tgcgggtgag cagggaatcc
     1981 gggttgacgc tgagatctcg aacgcggccc agcggccggc cgtcaacaaa cgtccgtgca
     2041 cgaagcttgt cgttcgccgc cagggcgaga ggtttctcat agaggttcga acccgtcgtc
     2101 ggctcggccc cgccgaccgt gtagcggatc tcgccgatag ccagactgga ggccaaggcg
     2161 acatcgacct tgccgccctc tccgggcgac agcttggcct ggggctcgaa gggaaccgcg
     2221 tcataggcca cgcccaaggc gtccaggcgc gcgaactcgg ccggcatgcg gcgattgaag
     2281 tcagcccagt cggtcgtggt ctgtggcgac cagccccgct cggccacggc gaccaggcgc
     2341 gggaagatca tgcgctccag ccgcacgtcg gtgcgcatgt gctcggtcca ggcgttgccc
     2401 tgcaggccga tgacgtgctt gcgctcgacc tcggtcattt ccttgggggc cgggtcgaag
     2461 tcgtagacgt ccttgaggct gaccacgcga ccccggcccg gaggctcgtc gggcgaggcg
     2521 ctctggcggt tgtccagata catcagcggc cagggcgaca gcaccgtgtc gtggcccagc
     2581 ttggcggcgg cgatcgcgcc ctccagaccg cgccaggaca tcacggtagc attgggggcc
     2641 agaccgcctt cgaggatctc gtcccagccg atcaggcgcc gaccgcgctt gtccagatgc
     2701 ttgccgatac gctggatgaa gtagctctgg gcttcgtgct catccttcag ggccagggcc
     2761 ttgatctggg cctgaatctt gggcgaagcc ttccactggt ccttcacggc ctcgtcgccg
     2821 ccgacgtgga tgtagtcgct ggggaagagg tccatgacct cgtccagcac gtcgttcagg
     2881 aaggcgaagg tgctgtcgtc gagattgtag agccagggga aaatgcccca gttgttgcgg
     2941 tcgatggccg gcggattcgg gttcacgccc aggtggggat aggcgacgat cggggccagg
     3001 gcgtgtcccg gcatttcgat ctcgggcacg atggtgacat tgcgggcggc ggcgtaggcg
     3061 accagttcgc gcacctcgtc ctgagtgtag aaaccgccat agcggatagc cttgccggtc
     3121 ttcggatccc tgcccgccgc gccggccggg gtgcgccagg cgcccacttc ggtcagcttg
     3181 ggatacttct tgatctccag ccgccagccc tggtcatcga ccaggtgcca gtggaaggtg
     3241 ttgagcttgt ggaccgccat ggcgtcgatg atgccgcgca gggtctcgac cgactggacg
     3301 tgacgggcgc tgtcgatcat caggccgcgc cagccaaagc gcggcgcatc ctcaatggcg
     3361 acagagggca gatcggccgg ccccttgccc gcctcctgcg tggccagctg ccagagggtc
     3421 atggcgccat agaacagacc cgcgcgcttg gcggcggtga tggtcacgcc ggacggacca
     3481 gattgcagag cgtagccctc gcctgtcgcg ccgccgcgcg ccaggacgat ggcggcctcg
     3541 ccggcggagg gcgcgccttc gacgacgacg ggcttcaggc ctcgggcgcg gaacagaaga
     3601 tcagaaagct gggcagcgac gcccctggcc tcgatatcgc ccttggcgac gaagatccgg
     3661 gtcttggcgg tcagcggaaa aacgccctct gcagtcgaga gcttggccgg catcggcgtc
     3721 agggccacag ggtctgccag cgccggtacg gcggcgacga gcgcgatggc ggacgccagg
     3781 gcggcgacgg atcggacgag gatcatgaac aggctcccag agatacaggc gcgcggcaaa
     3841 aagcgggcgg agcgacgagg cgccccgccc aagctccatg cttgcaggtc aggaggatgg
     3901 tgctggcggc gacgcagagc gagcgggtca tcaggtgatc ctgaaccatg ccgcagccga
     3961 tgcaatcgcc gtcggtagag atcgggactt ccgaaagagg ggtccggaag tcccgatggg
     4021 ctttagaact tgaagcggac gccggcgtag aaagtgcggc cgttgtcgta gatcgcgcgc
     4081 ggcacatcag gcgtgcccac gaagtacttc agcttctcgt tcagcaggtt ctgggcgtcg
     4141 aacgtcagcg cgatcgtctt gttgaggttg acgctgaccg agccgtccag ggtggcgaaa
     4201 ttgtcctgga acatgtcggt gccccgatcg gtgcccgact tgaacttcga gcgatagctg
     4261 taggccagtc gcgcgctgag caggtcgttc tcgaagtagc ccgtcaggtt gtaggtgtgc
     4321 ttcgaagagc catcgatcgg cttgcccagg tcggtcttgg cgtccgaata ggtgtagttg
     4381 ccgataaagc cgaagccgcc gccgatgggc tgctgcaggg tgacttcgaa gccgcggttg
     4441 gtgccgccgt ccccgttgat cggacccgtc atcgtgaagg tgtcaccctt gcccggaccc
     4501 agacgctgct gggtctgctg ggtgatgtgg aactcctggt aggtccggct ggtaatgtag
     4561 ctctcgatat ccagcaggaa gacgccgacc gacagcaggg cttcggggcc gtagtaccat
     4621 tcggcggcgg cgttatactg ccaggcgcgg aagggatcca gttcagggtt gccgccagca
     4681 gtgccggtga cggccgtatc gttcaggctg aaggcgccgg ccagctgggc atagcccgga
     4741 cgcgacatca ccttggcggc cgacaggcgg accacgacgt cttcgtcggc gttcacgacc
     4801 agggtcgcgc tgggcaggat atcccagtag tcgcgattga cgttgcgcgg gccaaacgaa
     4861 ccgaacacgt tggtgaccgt agcgggggtc accgaggcgg agtactgggt ggtgtcctgg
     4921 tcggtatgga cggcgcggac gccgacattg ccgcgccagc gatcgccgcc gagcttggcc
     4981 atcgcataga cggcctgggt cttttcctgc accgcgaagc tgagcggcgg atagtaggcg
     5041 aactgacggc cgccgttgcc agccagggcg gccagcacct tgccgcgatc ggccaggacg
     5101 tagccctgac ccgacaggcc gatgtcttcg ccatagttgg acggggtcga cttgccaacc
     5161 accgagccca gatcgacacc ggaagtgccg ctatagacgc cgttaccggc ccaggcatag
     5221 gcggtatagt tcacctcgcg ggtgtggtcg gtgtagcggc cgccgaacag cagggtcttc
     5281 aggacgccgt tgtcgaccgt gtattcaaca tcgcccttgg cgtagaattc ttcgtccggg
     5341 ctctcgacct tgccgcccca cgaccagctg tagaagttgt tcagataggc ggccgagccc
     5401 acagccggca gaccgggata gctgaccgag gtcaccggcg cgccgagctc gtagctcatg
     5461 ccggtttccc agtaggtttc ccaggcttcg gtgtcgtccg tggcgccttc gcccttggtg
     5521 taaccgatgt tgccgctgat cttcagattg tcggtcgcct gatactggcc atccaggttc
     5581 aggtcccacg aggtcgagtg cgcggtgcgg aagatgtcat cctgcaccat gccgcggctc
     5641 ttggttccgt tcggcgcggc ggccgccctg gtcgccgagt tgaggatgcc gttgctgatc
     5701 ttgtaggcgg tcagggcgtt gcccgccgaa cccgcaggac ccgtgctgcc ggtgatcaga
     5761 ttgctgaccc aggccatgcg gttcacgttg gtgttgtcag cgtccatctt ggtatagagg
     5821 cccgaaaacg acagctccag cttctcgttc ggctcccact gggtgacgaa gttggcgccc
     5881 ttgcgcttgc gggtctgctg gaagaaagcg ttaccgatca ggttcggcgc cagcaccgtc
     5941 tgacccgagc cggcaaagtt ctgatactcg gtatagccaa ggatttcctg accatcgcgg
     6001 cggaactcgc gctcctgata gaaggcgccg accagggcgc cgaacgtgct ggcctcgttc
     6061 ttccagctga ccatgcccga aacctggggc gtccacttct cggcgcgggt cgcatacaga
     6121 ccctggaccg aggcgttggc c
//
LOCUS       DAOMEO010000030         5922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1800_length_5922_cov_2.399182, whole genome shotgun sequence.
ACCESSION   DAOMEO010000030 DAOMEO010000000
VERSION     DAOMEO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1800_length_5922_cov_2.399182"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLH31_01300"
     CDS             <1..254
                     /locus_tag="PLH31_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQR87917.1"
                     /translation="DDVRREAMKEQTNIFRVLVKTLLNAGIITDRTKAFYAMYVDLDE
                     LRGEGERMVGDDIAEEGIRHLQAINFKDRPVNAVSIAAE"
     gene            342..884
                     /locus_tag="PLH31_01305"
     CDS             342..884
                     /locus_tag="PLH31_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR87918.1"
                     /translation="MRRLNPIPSGALACALVLGVMAGPALAGARPPAKPVSAEFYSGK
                     WYEIARTPNSGQRDCEAASTQFTSTGANGFKVVQICRKGSATGEAKTFNTTGAIVAAT
                     PRNAKFSMSFLGGLKKQEYWVLDRADDQSWAIMATPGGNYVWLLSRKAVMSPAARDAA
                     LARVRALGYAKLEFPAQPGA"
     gene            889..>1200
                     /locus_tag="PLH31_01310"
     CDS             889..>1200
                     /locus_tag="PLH31_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR87919.1"
                     /translation="MKPWPLLALPLLLAACVAGPSGNPNPPQPAKTVEIDRYLGRWYE
                     IARYDMRFEKGCEGVTAEYSKRPDGLIRVLNTCHQGAVDGPVKSSEGKARVVDTATNA
                     KL"
     assembly_gap    1201..1210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1211..1398
                     /locus_tag="PLH31_01315"
     CDS             <1211..1398
                     /locus_tag="PLH31_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR87920.1"
                     /translation="YWVMDRADDYSWAIVGEGSGKYLWLLTRKPPTDLEREALTARAK
                     AMGYDTAMLRPTLQPAP"
     gene            complement(1395..2489)
                     /locus_tag="PLH31_01320"
     CDS             complement(1395..2489)
                     /locus_tag="PLH31_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87921.1"
                     /translation="MTIFVSSAGASTAQVVFSEPETVAYASGETPSFFAMTPAKVGLI
                     GGLVGGVVMYATGAELIKKNGVPDPSADMARDLAQRLAERRMARLAGQPILTNVKKPT
                     AVAAAGAGAQYVLTVQTTNWGHLYYPLDWARYSVFYNAKMQVIDVPTRTVIHGELCSW
                     RSPKPGSPTRAELLRNNAEGLKAFVATAADVCFHQFERGLDKIAPPASRSTDRTLAAN
                     LNAGLAPAVTPSPEDQPVSPMPAAMATLVESRLPLPPVTPVAPPEAVSLSFLPPARNE
                     TSAEPLAVAATGVTTVAPRSLAPVVQAPAVEIAQTERPVAQAQPLPPYVEYPYARPHE
                     APPSRKPPPPEYRYAGRDADGFLTWPGKRP"
     assembly_gap    2651..2660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2685..3137
                     /gene="mmcB"
                     /locus_tag="PLH31_01325"
     CDS             2685..3137
                     /gene="mmcB"
                     /locus_tag="PLH31_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair putative endonuclease MmcB"
                     /protein_id="HQR87922.1"
                     /translation="MDVVIDLRPSRPETTDAVTRGAARFLVSLGYAPLAEVTLPNGRR
                     ADLMALGRKGDIIIVEVKSGLEDFRIDRKWPDYAPYCDGFYFAVAPYFPEGILPDEPG
                     LIVADAFGGGVIREAPVAPLAPARRKALTLAFARLGAMRAAGVKADTL"
     gene            complement(3134..4978)
                     /gene="recQ"
                     /locus_tag="PLH31_01330"
     CDS             complement(3134..4978)
                     /gene="recQ"
                     /locus_tag="PLH31_01330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HQR87923.1"
                     /translation="MPTIVDSAALETARDVLRRTFGHADFRGLQAGVIGELLAGRSAM
                     AVLPTGGGKSLCYQIPALIRPGLGLVVSPLIALMADQVAGLQQAGVAAERLDSNTLPA
                     ERADIWRRIDEGQLDLLYLSPEGLMQPSMLDRLSRLNLALVAVDEAHCVSQWGHDFRP
                     EYRMLGRIAQVFPNVPRLAVTATADARTRDDIRAELRLEGAAEFVDSFARPELALNAE
                     RKRGKGHDRVVELVLERPGRAGVVYAGSRDSTEKLAEKLIAQGVPALAYHAGLDKNVR
                     ARRLEEFLEADAAVMVATIAFGMGVDKPDVRFVIHADPPAAIEAYWQEIGRAGRDGQP
                     AEGITLYGSADMAWASKRIEGREAPDEVRQVQTRKLRQFYAMLEGTTCRAAAVRRYFG
                     EEGVAVCGVCDICTSPPQGIDATEAAQKALSAAHRLGGRFGRGRLIDHLLGKTKDVTP
                     QEAQMSTFGIGREYSPGTWRDLLDTLVFEGLLREDPNDGRPLIGLGEVDGVKAVYRGE
                     RRVSLRQQVEASDPTTRSGAPRKRREGKALTVPLEDQALFEALRAWRKEQAQAQHVPP
                     YVIFHDATLAEIAAARPASLPALAKAGGVGQGKLDRYGEAVLKVVREN"
     gene            5209..>5922
                     /locus_tag="PLH31_01335"
     CDS             5209..>5922
                     /locus_tag="PLH31_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQR87924.1"
                     /translation="MANGTFGGNRPATPTRTPLQALVYWGAVLSLWGLIFVIAFFAVF
                     AWDLPDTSKLYDVKRQPSINYLDRSGALLAVRGSQYAPPVDIDALPKYVPAAFVAIED
                     RQFYRHFGFNPWGIIRSLAWNMTHDGPQRGGSTITQQLARNLFLSPAQNYRRKAQELI
                     LAVWLELKFSKKQILALYMNRVYFGAGAYGIEAASQRYFNKPANQLTIGESALLAGMM
                     KGPSRYSPVSATDRAARRAT"
BASE COUNT          988 a         2008 c         1957 g          949 t
ORIGIN      
        1 ccgacgacgt gcgtcgcgag gccatgaagg agcagaccaa catcttccgg gtgctggtca
       61 agacgctgct gaacgccggc atcatcaccg accgcaccaa ggccttctac gccatgtatg
      121 tcgacctcga cgaactgcgc ggcgagggcg agcggatggt gggcgacgac atcgccgagg
      181 aaggcatccg ccacctgcag gccatcaact tcaaggatcg cccggtgaac gcggtcagca
      241 tcgccgcaga atagacacgc gattgcgcga gacacgccgt ccgcccggaa caccgggcgg
      301 acggtttgcg tttgagttcc cttgttggca aggtttcttc catgcgtcgt ctcaacccga
      361 tcccttctgg cgcgctcgcc tgcgccctgg tccttggcgt catggccggt ccggccctgg
      421 ccggcgccag gccgcccgcc aagcctgtct cggccgaatt ctattcgggc aaatggtacg
      481 agatcgcccg cacccccaat tccggccagc gcgactgcga ggcggcgtcc acccagttca
      541 ccagcaccgg cgccaatggc ttcaaggtcg tgcagatctg tcgcaaaggc tcggccaccg
      601 gtgaggccaa gaccttcaac accactggcg cgatcgtggc cgcaacgcct cggaacgcca
      661 agttctcgat gagctttctg ggcggcctga agaagcagga atactgggtg ctggaccgcg
      721 ccgacgacca gtcctgggcg atcatggcca cgcccggcgg caactacgtg tggctgctgt
      781 cgcgcaaggc cgtgatgagt ccggccgcca gggacgctgc tctggccagg gtcagggcgc
      841 tgggctacgc caagctcgaa ttccccgccc agcccggagc gtgatcgcat gaagccctgg
      901 ccccttctgg ccctccccct gctgctggcc gcctgcgtcg cgggccccag cggcaatccc
      961 aatccgcccc agccggccaa gaccgtcgag atcgatcgct atctgggtcg ctggtacgag
     1021 atcgcccgct acgacatgcg gttcgagaag ggctgcgagg gcgtcaccgc cgaatattcc
     1081 aagcgtcccg acggcctgat ccgggtgctc aacacctgcc accagggcgc ggtcgatggg
     1141 ccggtcaaga gcagcgaggg caaggccagg gtggtcgaca cggccaccaa cgccaagctg
     1201 nnnnnnnnnn actactgggt catggaccgc gccgatgact atagctgggc gatcgtcggc
     1261 gagggctcgg gcaagtatct gtggctgctc actcgcaagc cgcccactga cctggagcgc
     1321 gaggccctga ccgcccgggc caaggccatg ggctatgaca ccgcgatgct gcgcccgacg
     1381 ctgcagcccg cgccttaagg ccgcttgccc ggccaggtca ggaagccatc ggcgtcgcgg
     1441 ccagcgtagc gatactcggg cggcggcggc ttgcgtgacg gcggcgcctc atggggccgg
     1501 gcatagggat attcgacata gggcgggagc ggctgagcct gagctacggg gcgctcggtc
     1561 tgcgcaatct cgacggctgg agcttgaacg acaggcgcca atgaacgcgg agcgacggtc
     1621 gtgacgccgg tggctgcgac tgccagtggc tcagcgctcg tctcgttgcg cgctgggggc
     1681 aagaacgaga gggaaacggc ttccggaggc gcaacaggcg tgacgggcgg caatgggaga
     1741 cgactttcga cgagcgtcgc catggcggct ggcattggcg atacgggctg gtcctccgga
     1801 gagggtgtga ccgccggcgc caaacctgcg ttaagattcg ccgccaaagt gcgatccgtc
     1861 gatcgtgacg ccggtggcgc gatcttgtcc aatccccgtt cgaactggtg aaagcagacg
     1921 tcagcggccg tcgcgacaaa ggctttcagg ccttcggcgt tgttgcgaag gagctcagca
     1981 cgggtcggcg aaccaggctt gggtgaccgc cagctacaga gttcgccgtg aatgaccgtc
     2041 cgggtcggca cgtcgatcac ctgcattttg gcgttgtaga aaaccgaata gcgcgcccag
     2101 tcgagcggat agtacagatg gccccaattc gtggtctgca ccgtcagaac gtactgggcg
     2161 ccagcccccg ccgcagccac agctgtcggc tttttgacat tggtgaggat tggctgcccg
     2221 gcgaggcgcg ccatacgccg ctctgcaagt ctctgggcca gatcgcgcgc catatctgcc
     2281 gatggatcgg gcacgccgtt cttcttgatg agctcagcac ccgtggcgta catcacgacg
     2341 ccgccgacca acccgccgat caaccctact ttcgcgggtg tcatcgcgaa aaagctcggt
     2401 gtttcaccgc tagcgtaggc gacggtctct ggttcggaga acacaacctg cgcagtagag
     2461 gcgccagcgg acgacacgaa gatcgtcatt gccgtggccg tcagaacaaa ggactttccc
     2521 accagaccac ctcacgcccc acttgctgcg tccgagctat tgcgccgaaa ggttgtggtc
     2581 aaagcgtcgc cctcgacgag ggaagccagc tcgcccggcg ggatgctatt taaccttgat
     2641 ccgcgaacaa nnnnnnnnnn acaaagctgg aacaggatcg cgccatggac gtcgtgatcg
     2701 acctgcgccc gtcgcggccc gagaccaccg acgcggtcac ccgcggggcg gcgcgattcc
     2761 tggtttcgct gggctatgcg ccgctggccg aggtgacgct gcccaatggc cggcgggccg
     2821 acctgatggc cttgggccgc aagggcgaca tcatcatcgt cgaggtgaag tcggggcttg
     2881 aagacttccg catcgatcgc aagtggccag actatgcccc ctattgcgac ggcttctatt
     2941 ttgcggtggc cccgtacttt cccgagggaa tcctgcccga tgaacccggc ctgatcgtcg
     3001 ctgacgcctt tggcggcggc gtgatccgcg aggcgccggt cgcgccgctg gcgccggccc
     3061 gccgcaaggc cctgaccctg gccttcgcac ggctaggggc gatgcgggcg gctggcgtca
     3121 aggccgacac cctctagttt tcccgcacca ccttcagcac cgcctcgcca taccggtcca
     3181 gcttgccctg acccacgccg ccagccttgg ctagggccgg caggctggcc ggtcgcgcgg
     3241 cggcgatctc ggccagggtg gcgtcatgga agatgacgta gggcggcaca tgctgggcct
     3301 gggcctgctc tttgcgccag gcgcgcaggg cctcgaacag ggcctgatcc tccagaggca
     3361 cggtcagggc cttgccctcg cggcgcttgc gcggcgcacc agagcgggta gtcgggtcgg
     3421 acgcctcgac ctgctggcgc aggctgaccc gccgctcgcc gcgatagaca gccttgacgc
     3481 cgtcgacttc gcccaggccg atcaagggcc ggccgtcatt gggatcctcg cgcagcaggc
     3541 cttcgaacac cagggtgtcg agcaggtcgc gccaggttcc agggctatat tcgcggccga
     3601 tgccgaaggt cgacatctga gcttcctgcg gcgtgacgtc cttggtcttg cccagcaggt
     3661 ggtcgatcag gcggccgcgc ccgaaccgtc cgcccaggcg atgggccgcc gacagcgcct
     3721 tctgggccgc ctcggtggcg tcgatcccct ggggcggcga ggtgcagata tcgcagaccc
     3781 cgcagacggc cacgccttct tcgccgaaat agcggcgcac ggcggcggcg cggcaggtgg
     3841 tcccctccag catggcgtag aactgccgca gcttgcgggt ttggacctgg cgaacctcgt
     3901 cgggggcctc gcggccttcg atgcgcttgc tggcccaggc catgtcggcc gagccataga
     3961 gggtgatgcc ttcggccggc tggccgtcgc gcccggcccg gccgatctcc tgccaatagg
     4021 cctcgatggc ggccggcgga tcggcgtgga tcacaaagcg gacgtcaggc ttgtcgacgc
     4081 ccatgccgaa cgcgatcgtc gccaccatca ccgcggcgtc ggcctcaagg aactcctcaa
     4141 gccggcgggc gcggacgttc ttgtcgagac cggcgtgata ggcgagcgcg gggacgccct
     4201 gggcgatcag tttctcggcc agcttctcgg tggagtcgcg gctgccggca tagacgacgc
     4261 cggcccggcc cgggcgctcc agcaccagct cgacgacgcg atcgtgacct ttgccgcgct
     4321 tgcgctcggc gttgagggcc agttcgggcc gggcgaagct gtcgacgaac tcggccgcgc
     4381 cttccaggcg cagctcggcg cggatatcgt cgcgggtccg ggcgtcggcg gtggcggtga
     4441 cggccaggcg cgggacattg ggaaacacct gagcgatgcg gcccagcatc cggtattcgg
     4501 gccggaaatc atgaccccac tggctgacgc agtgggcctc gtcgaccgcc accagggcca
     4561 ggttcaaccg cgacagccga tccagcatcg agggctgcat caagccctca ggcgacaggt
     4621 acagcagatc gagctgcccc tcgtcgatgc gccgccagat atcggcgcgt tcggcgggca
     4681 gggtgtttga gtccagccgc tcggccgcga cgccggcctg ctgcaggccc gccacctggt
     4741 cggccatcag ggcgatcagc ggcgagacca ccaaaccgag gcccgggcgg atcagggcgg
     4801 ggatctggta gcacaggctc ttgccgccgc cggtcggcag caccgccatg gcgctgcgcc
     4861 cggccagcag ctcgccgatg acgcccgcct gcagcccccg gaagtcggca tggccaaacg
     4921 tccggcgcag cacgtcgcgg gcagtttcaa gagcggcgga atcaacgatc gtgggcacgg
     4981 cggacttgtg acagcggcga tgcgcagcgc caagccattc ctcgcgcggc aaccgaaacc
     5041 aagatcatct gactcaacct aaaatcgcaa tctcgaaaat gcgccggcgc gccacaacac
     5101 cgccgcgttc agcgttcacg tctcgtcaat cttgtcgcgc gtttggtggg cttcggctaa
     5161 acggggcgca ggcgagcgcc gttcgaagca ccctgggata gagacgacat ggccaacggt
     5221 accttcggcg ggaacaggcc ggcgacgccg acgcgtacgc cgctgcaagc cctggtctat
     5281 tggggggcgg tgctcagcct ctggggcctg atcttcgtca tcgccttctt cgcggtcttc
     5341 gcctgggacc tgcccgatac gtccaagctg tatgacgtca agcgccagcc ctcgatcaac
     5401 tatctggacc gctccggcgc cctgctggcc gtgcgcggca gccagtatgc gccgccggtc
     5461 gatatcgacg ccctgccaaa gtacgtcccg gccgccttcg tggccatcga ggaccgccag
     5521 ttctatcgcc acttcggttt caatccctgg ggcatcatcc gctcgctggc ctggaacatg
     5581 acccatgacg gtccccagcg cggcggctcg acgatcaccc agcagctggc gcggaacctg
     5641 ttcctgagcc cggcccagaa ctatcgccgc aaggcccagg agctgatcct ggcggtctgg
     5701 ctggaactga agttcagcaa gaagcagatc ctggccctct atatgaaccg ggtctatttc
     5761 ggggccggcg cctatggcat cgaggccgcc tcgcagcgct atttcaacaa gcccgccaac
     5821 cagctgacca tcggcgagag cgccctgctg gccggcatga tgaagggccc ctcacgctat
     5881 tcgccggtct cggccaccga ccgcgccgcc cggcgcgcca cc
//
LOCUS       DAOMEO010000031         5862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1813_length_5862_cov_2.770277, whole genome shotgun sequence.
ACCESSION   DAOMEO010000031 DAOMEO010000000
VERSION     DAOMEO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1813_length_5862_cov_2.770277"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1151)
                     /locus_tag="PLH31_01340"
     CDS             complement(15..1151)
                     /locus_tag="PLH31_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR87925.1"
                     /translation="MRPDLDPFHAIAISRLAHQLKSEGRSVIHMEFGQPSTGAPKAAI
                     ECAHHVLDTDGLGYWESPALKARIARHYDDKHGVHVDPEQIILTCGASPALVMALASA
                     FEAGDRVALARPGYVAYRNTLKALNLVPVEIACGPEDRYQLTAAHLAALDPAPAGVIV
                     ASPANPTGTIIAEAEMAAIAEVCRTRNIRIVSDEIYHGLSYVGPARSTLEFEPTALVV
                     NSFSKYFSMVGWRLGWLLSPLDHVHRARAYMGNLFLTAPSLSQHAGLIAMDCAEELEG
                     HVEVYRKNRQLLLDALPSLGLAKIAPPDGAFYIWADIGHLTDDSMAFCKQLLTDTGVA
                     TAPGVDFDPVEGHRFMRFSFAVSTAEVEEALRRLVPWFGGRSKI"
     gene            1217..2257
                     /locus_tag="PLH31_01345"
     CDS             1217..2257
                     /locus_tag="PLH31_01345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQR87926.1"
                     /translation="MRSILLALVGVLSLSAAPVAAQQTALTPHIGGRVAPAAEGGLLF
                     GWPGVYFEGRFSGPSVTVAVDSGSEHLTISIDGQVRAELLKPGLARLTFDQLAPGEHV
                     VRLDKLTESQTGRARFVGFFVGPEGRPLAPVMRPRRIEFIGDSYTVAYGVRSNRFDCT
                     EQQVHDLTDTSQSFAPVLAGRLNADYRIIAFSGRGVVRNYSGLAPGEPLPALYSRLIP
                     GQAEPRVDPADPWRPDLIVVGLGTNDFSTPLKADEGWKTEADLRADYRDSYVTFVAAL
                     ARRHPQARFLLIAGDTFADDVEAVVKRLNGASAGLATAVRITGLERTACHGHPSAADG
                     RMIADRLEAAIQ"
     gene            complement(2284..>4484)
                     /gene="rnr"
                     /locus_tag="PLH31_01350"
     CDS             complement(2284..>4484)
                     /gene="rnr"
                     /locus_tag="PLH31_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQR87927.1"
                     /translation="KADIARHFGLKGADRRALREMLRELEAQGDLGKRGRKGFAEAGS
                     LPPVGVADVVDRDNDGDMFVRLTKAGEDAPLVRLSPARNDNAGGAPGMGDRVLVRFER
                     LENGEMEARLIKRLGQSAHKILGVIRKRRREIRVEPVDRKSKEVLVLSEHDARDLKDG
                     DLVLASVGMATGRYGPKQGKVLEVVGSEDDPRAASLIAIHSHGIPVGFSEEAEAEASA
                     AQPPTLEGRDDLRDLPFVTIDPVDARDHDDAVYAHPDPDAGNAGGWIVWVAIADVAAY
                     VRPGSALDREAREKGNSVYFPDRVEPMLPEVLSNGLCSLREGENRATLAVRMVFDSAG
                     RKKSHRFVRGLMRSAAKLSYEQAQAAIDGVIPPDGQDADKAGPLLDPILKPLWAAFRL
                     MGKGREIRSPLAIESDERKIVITQEGEIASINRRASLEAHKLIEEMMIQANVSAAESL
                     EAKRTPLIYRVHDTPSQEKVQNLAEFLATLEIKWNKGEAPQTSRFNKLLDETREGPHA
                     AIVNEVVLRTQMQAHYSADNLGHFGLNLAKYAHFTSPIRRYADLIVHRALIRALNLGT
                     DGLTDQDISQIKDTAETITYAERRAMAAERDATDRYIAAFLSDRVGATFEGRITGVTR
                     FGLFIKLSETGADGLIPVSTLGQEFFVHDERMHALVGERTGKRWPLGLAVEVKLVEAT
                     PVTGGLLFEMLSEPVAADPNAPRPRLGARRQAAVKPRGGLPKGVRRGKRR"
     assembly_gap    4485..4528
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4652..4915)
                     /locus_tag="PLH31_01355"
     CDS             complement(4652..4915)
                     /locus_tag="PLH31_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87928.1"
                     /translation="MRLAVAASILFATVAAGACTDSKRASRDATWGDKPADIVCWSYG
                     TQTFAGRSTGKVEYDEGGRISFVDAANGRYTTVEGDCRVTYSR"
     assembly_gap    4995..5004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5034..5435
                     /locus_tag="PLH31_01360"
     CDS             5034..5435
                     /locus_tag="PLH31_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87929.1"
                     /translation="MVVSIFKVTLGVVVSCLLAMPGMAHSAQAELPEVSNAIEYASVS
                     DAREALKVRPGVVFSLMNGWDIAIDEAARTVWSFSPAHYPAYPAVVKRQVLQDAGKVY
                     IKMSVHCEASKAVCDDLVRTFADMTGLESPR"
     gene            complement(5464..5829)
                     /locus_tag="PLH31_01365"
     CDS             complement(5464..5829)
                     /locus_tag="PLH31_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87930.1"
                     /translation="MTTLDRPLPLTVVVAALILASGLGFAMGLTNALKAGKSNVTAST
                     APLAEVSGVPVKDAAPAPLYEPPPPEKPKVKAVEVKTAEIEAPVAVIEAPPAPSESAP
                     PATAPAPVQPPPQSIEDLY"
BASE COUNT          896 a         1883 c         2022 g         1007 t
ORIGIN      
        1 gcaaggggag aaggctagat tttcgaccgt cccccaaacc acggaaccag ccgccgcagc
       61 gcctcctcga cctccgccgt cgagaccgcg aaggaaaacc gcatgaaccg gtggccctcg
      121 acggggtcga agtcgacgcc cggtgcggtg gcgaccccgg tgtcggtcag caactgtttg
      181 cagaacgcca tcgagtcgtc ggtcaggtgg ccgatatcgg cccagatgta gaacgccccg
      241 tccggcggcg cgatcttcgc caggcccaag gaggggaggg cgtccagcag cagctggcgg
      301 ttcttgcgat agacctcgac atggccttcc agctcttcgg cgcagtccat ggcgatcagg
      361 ccggcgtgct ggctcagcga cggggcggtg aggaacaggt tgcccatata ggcccgcgcc
      421 cgatggacat ggtccagcgg cgacaacagc cagcctaacc gccagcctac catgctgaaa
      481 tatttggaga aactgttgac caccagggcg gtgggctcga actccagcgt cgagcgggcg
      541 gggcccacat aagacaggcc gtgatagatt tcgtcgctga cgatgcgaat attccgtgta
      601 cggcatacct ctgcaatggc cgccatttcg gcttcggcga tgatggtgcc ggtcgggttg
      661 gcggggctgg cgacgatgac gccggccggg gcggggtcta gcgccgccag gtgcgcggcg
      721 gtcagctgat agcgatcctc cggtccgcag gcgatctcga ccgggaccag gttcagggcc
      781 ttcagcgtat tgcgataggc cacatagccc ggcctggcca gggccacacg gtcgccggcc
      841 tcgaacgccg aggccagggc catgaccagg gccggcgagg cgccacaggt caggatgatc
      901 tgctcgggat cgacgtgaac gccgtgcttg tcgtcatagt gcctggcgat ccgcgccttc
      961 agggccggac tttcccaata gcccaggccg tcggtgtcga gcacgtggtg ggcgcattcg
     1021 atggccgcct tgggcgcgcc ggtcgagggc tggccaaact ccatgtggat caccgagcgg
     1081 ccttcgctct tcaactggtg agccaggcgg ctgatggcga tggcgtggaa cgggtcgaga
     1141 tcggggcgca tgatttcctg ttagcgggga tcgcgccgtc atgacagatg gagcggtgat
     1201 ctgaccagag ccgttcatgc gatccatcct actcgccctc gtcggcgtcc tgtcattgtc
     1261 ggccgcgccg gtggccgccc aacaaacggc cctgacgccc catatcggcg gacgggtcgc
     1321 gccggccgcc gagggcggac tgctgtttgg ctggcccggg gtctatttcg aggggcgctt
     1381 ttccggcccg tcggtgaccg tggcggtcga ttccggtagc gagcacctga cgatcagcat
     1441 cgacggtcag gtccgcgccg agctgctcaa gccgggtctg gcccgcttga ccttcgatca
     1501 gttggcgccg ggcgagcatg tggtccgcct ggacaagctg accgaaagcc agacgggacg
     1561 cgcgcgcttc gtcggcttct tcgtcgggcc ggagggacgg cccctggcgc cggtcatgcg
     1621 gccccgccgg atcgagttca tcggcgactc ctacacggtg gcctatggcg tgcggtcgaa
     1681 ccgcttcgac tgcaccgaac agcaggtcca cgatctcacc gacaccagcc agtcgttcgc
     1741 cccggtcctg gccggacggc tgaacgccga ctaccgcatc atcgccttct ccgggcgggg
     1801 cgtagtgcgc aactacagcg gcctggcccc gggcgagccc ctgccggccc tctattcgcg
     1861 cctgataccc ggacaggccg agccgcgcgt cgatccagcc gatccctggc ggccagacct
     1921 gatcgtggtg ggcttgggca cgaacgactt ctcgaccccg ctaaaggccg acgagggttg
     1981 gaaaaccgag gccgacctgc gcgccgacta tcgcgattcc tacgtgacct tcgtggccgc
     2041 cctggcgcgc cgccatccgc aggcgcggtt cctgctgatc gcgggcgaca ccttcgccga
     2101 tgacgtggag gcggtcgtca agcggctgaa cggggcgagc gcgggtctgg cgacggccgt
     2161 gcggatcacc ggccttgagc gcaccgcctg ccacggccat ccctcggctg ccgatgggcg
     2221 gatgatcgcg gatcggctgg aagcggcgat ccagtaggcc ctgccccggt agagggagaa
     2281 tgtttagcgc cgcttccccc gccgaacgcc cttgggcagc ccgccgcgcg gcttcacagc
     2341 cgcctgccgc cgggcgccaa gacgaggcct tggcgcattc ggatcggccg ccaccggctc
     2401 gctcagcatc tcgaacagca ggccgccggt gaccggcgtg gcctcgacca gcttgacctc
     2461 cacggccagg cccaacggcc agcgcttgcc ggtgcgttcg ccgaccaggg cgtgcatccg
     2521 ctcgtcatgg acgaagaatt cctgacccag cgtcgagacc gggatcaggc cgtcagcgcc
     2581 ggtctcgctc agcttgatga acaggccaaa gcgcgtgacg ccagtgatac gtccctcgaa
     2641 ggtcgctccc acccggtcgg acaggaaggc ggcgatatag cggtcggtgg cgtcgcgctc
     2701 ggcggccatg gcccggcgct cggcataggt gatggtctcg gccgtgtcct tgatctggct
     2761 gatgtcctgg tcggtcaggc catcggtccc caggttcagg gcgcggatca gggcgcggtg
     2821 cacgatcagg tcggcatagc ggcggatcgg gctggtgaag tgggcgtact tggccaggtt
     2881 caggccgaaa tggcccagat tgtcggcgct gtagtgggcc tgcatctggg tgcgcagcac
     2941 cacctcgttg acgatggcgg cgtgcggccc ctcgcgggtc tcgtccagca gcttgttgaa
     3001 gcgcgaggtc tgtggggcct cgcccttgtt ccacttgatc tccagcgtcg ccaggaactc
     3061 ggccaggttc tgcaccttct cctggctggg cgtgtcgtgg acgcgataga tcagcggcgt
     3121 acgcttggct tccaggctct cggcggcgct gacattggcc tggatcatca tttcctcgat
     3181 cagcttgtgg gcttcgaggc tggcgcggcg gttgatcgag gcgatttcgc cttcctgtgt
     3241 gatcacgatc ttgcgctcgt cgctctcgat ggccagcggc gagcggatct cgcgaccctt
     3301 gcccatcagg cggaaggcgg cccacagggg tttcaggatc ggatccagca gcggaccggc
     3361 cttgtcggcg tcctggccgt cgggcgggat cacgccgtcg atggcggcct gggcctgttc
     3421 gtaggacagc ttggcggcgc tccgcatcag gccgcgcacg aacctgtggc tcttcttgcg
     3481 accggcgctg tcgaacacca tccgcacggc cagcgtcgcc cggttctcgc cttcgcgcag
     3541 gctgcacagg ccgttggaca gcacctcggg cagcatcggc tcgacgcggt cggggaagta
     3601 gacgctgttg cccttctcgc gggcttcgcg gtccagggcc gagcccgggc gcacataggc
     3661 ggcgacatcg gcgatggcca cccagacgat ccagcccccc gcattgccag cgtccggatc
     3721 gggatgggcg tagacggcgt cgtcgtggtc gcgggcgtcc acggggtcaa tggtcacgaa
     3781 cggcaggtcg cgcaggtcgt cgcggccctc cagggtgggg ggctgggcgg cggaggcttc
     3841 ggcttccgcc tcttcggaga agccgaccgg gatgccgtgg ctgtggatgg cgatcaggga
     3901 ggcggcgcgg ggatcatcct cgctgcccac cacttccaga accttgccct gcttggggcc
     3961 atatcggccc gtggccatgc ccaccgaggc cagcaccagg tcgccgtcct tcaggtcccg
     4021 ggcgtcgtgc tctgagagga ccagcacttc tttcgactta cggtccaccg gctcaacccg
     4081 gatctcgcgg cgacgcttgc ggatgacgcc caggatcttg tgagcgctct ggcctaggcg
     4141 cttgatcagc cgggcttcca tctcgccgtt ttcgaggcgc tcgaagcgga ccagaacccg
     4201 gtcgcccatg cccggcgcgc cgccggcatt gtcgttgcgc gcgggtgaca ggcgcaccag
     4261 cggcgcatcc tcgccggcct tggtcaggcg cacgaacatg tcgccgtcat tgtcgcgatc
     4321 caccacgtcg gccacgccca cgggcggcag cgagccggct tcggcgaagc ccttgcggcc
     4381 gcgcttgccc agatcgccct gggcttccag ttcgcgcagc atctcgcgca gagcgcggcg
     4441 atcggcgccc ttcagaccaa agtggcgagc gatgtcggcc ttgcnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gaaatttcga agacttgggc gtgcgcggtt
     4561 tggccatgtg ctgggataac ccggcagcgg gggggaaggc caagggaaac gcgctgaaac
     4621 cggcagcgat ctgctaccgg acgatgatgg actatctcga ataggtgacc cggcagtcgc
     4681 cttctaccgt ggtgtagcgg ccgttggcgg cgtcgacgaa cgagatccgg ccaccctcgt
     4741 catactcgac cttgccggtc gagcggccgg cgaaggtctg cgtcccatag ctccaacaga
     4801 cgatgtccgc cggcttgtcg ccccaggtcg catcgcgact ggcccgctta ctgtcggtgc
     4861 aggcgcccgc agcaaccgtg gcgaaaagga tcgaggcggc aacggcaagg cgcattgtgg
     4921 gtctcacagg aaagccccgg ctggtggggc gatgaacggg tcagtctcga gcgtaggcga
     4981 caatggacgg ggcgnnnnnn nnnngggcgc tgtcggcgct atagcttcaa tctatggtcg
     5041 tttctatatt caaagtcacg cttggggttg tggtgagctg tctactggcg atgcccggga
     5101 tggctcattc ggctcaggct gaacttcccg aggtttccaa cgcgatcgaa tatgccagcg
     5161 tatcggatgc gcgggaggcc ttgaaggtca ggccgggcgt cgttttcagt ctgatgaatg
     5221 gatgggatat cgcgatagac gaggccgccc ggacagtctg gtcgttttcg cctgcccact
     5281 atccagccta tcccgccgtc gtgaaacggc aggttctgca agacgctggc aaggtctata
     5341 tcaagatgag cgtccactgc gaggcgtcga aagccgtttg cgacgacctt gtccgaacct
     5401 tcgccgacat gacgggcctg gaatcgccgc gataaggaaa gcccgtttcc ggcgggtggc
     5461 ggatcagtaa aggtcttcga tcgactgggg cggcggctgg accggcgcag gcgccgtcgc
     5521 tggcggcgcg gattccgatg gggcaggcgg ggcctcgatc acggcgacgg gcgcctcgat
     5581 ctcggcggtc ttgacctcga cggccttgac cttgggcttc tcgggcggcg gcggttcgta
     5641 gagcggggcc ggcgccgcgt ccttgacggg cacgcccgaa acctcggcca ggggcgcggt
     5701 cgaggcggtg acgttcgact tgccggcctt cagggcattg gtcaggccca tggcgaagcc
     5761 caggccgctg gccaggatca gggcggcgac cacgacggtc agcggcaggg gacggtcaag
     5821 cgtcgtcatg ccttcaatct aaccccggtt cgcggtcatg gg
//
LOCUS       DAOMEO010000032         5829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1828_length_5829_cov_2.648078, whole genome shotgun sequence.
ACCESSION   DAOMEO010000032 DAOMEO010000000
VERSION     DAOMEO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1828_length_5829_cov_2.648078"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /gene="sfsA"
                     /locus_tag="PLH31_01370"
     CDS             complement(<1..704)
                     /gene="sfsA"
                     /locus_tag="PLH31_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQR87931.1"
                     /translation="MLLPQPLVRGRLVQRYKRFFADIVLDDGRAITAHCPNPGAMLGV
                     NLPGQGAWVSWSDDPKRKLPWTLQLVEANGGPQGSVLVGVNTMLPNKLVAEALAADAI
                     PELSGYATLRPEVKYADASRVDFLLTGPDRAHCWLEVKNVHLSRTPGLAEFPDCVAAR
                     STRHLADLAAQVSAGDRAVALFVVQREDCESFKACADLDPAFARGLAQAHAGGVEILV
                     YACDVTIEGVRITHRLR"
     gene            758..1525
                     /locus_tag="PLH31_01375"
     CDS             758..1525
                     /locus_tag="PLH31_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQR87932.1"
                     /translation="MTASKDRVTAAVLIIGDEILSGRTQDVNLSAIAKYLATYGVDLS
                     EARVVPDVEQEIIDAVNALRTKYDYVITTGGIGPTHDDITADSVAKAFGVTAPEHPEI
                     MALLRERWGEPNAARRRMAHVPEGGVLVKNPVQGPPGFQIENVFVLAGVPAIMRGMLG
                     DVGPRLRTGAVVISRTVRVTGSGEGLIAAPLEAVAKAHPDMSLGSYPFFSPPDIYGAN
                     LVVRGRDPAEVDTAVEELVVALTEAGAAQIERIAPDA"
     gene            1685..2292
                     /locus_tag="PLH31_01380"
                     /pseudo
     CDS             1685..2292
                     /locus_tag="PLH31_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519106.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     assembly_gap    2293..2302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2303..2880
                     /locus_tag="PLH31_01385"
     CDS             <2303..2880
                     /locus_tag="PLH31_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQR87933.1"
                     /translation="AFSGAAKISLDYAVMERTQKAAVVPAAFSWSDLGAWDAIWDAAA
                     RDEAGNAVFGDGTLIDSRNVLIRSTGPFVGVIGCEDLVVVAEPDAILVCRRDASQSVK
                     TLVDGLKRDGRSIASGRAREGAPRRVLATTAGARVEQWTLKASETVELPIGTVQVLEG
                     AVEGLNALAAIDAVTQVTASVATVLLVTRKL"
     gene            3363..3737
                     /locus_tag="PLH31_01390"
     CDS             3363..3737
                     /locus_tag="PLH31_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR87934.1"
                     /translation="MRRILILSLLLVAGCAPSSPSGLTPVTAAATPVVLADPAARGRV
                     LVEARCATCHAVEAVGDSPVAAAPPLRTLGQRYPVANLQEAFAEGISTGHPQMPQFVL
                     EAGQVQDLIAYLESIQIPARKS"
     gene            3796..5082
                     /locus_tag="PLH31_01395"
     CDS             3796..5082
                     /locus_tag="PLH31_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR87935.1"
                     /translation="MNMRVGIKAVAIAAAFCAAGSAQAAEITAVSAARLLDVASGKYV
                     DNPMVIITDGRITAVGKQGEVAVPAGAKVTDLPGATLLPGLIDMHVHIDSLAEVGGYN
                     SLQYSDAFWSVVQTANARATVEAGFTTIRNLGADRFNDVGLREAIEAGYVPGPRIVPA
                     AYSIGATGGHCDSTYFPPSMAQKGPYVADGPEQGRQKVREVRKYGARVIKICATGGVF
                     SRNTEPGQQQLSLAEMTAIVDEAHMWGLKVAAHAHGAAGIKDAIRAGVDTIEHVSLVD
                     DEGIKLAVQKGAWFSMDIYNTDYTQAEGKKNGVLEDNLRKDREIGDVQRENFRKAVKA
                     GVKMVLGTDAGIYPHGQNAKQFAVMVRYGATPLQAIQAATVNAAQALGQEKDVGQVAV
                     GRYGDIIAVTGDPLADVTTLEKPVFVMKGGAVVRQP"
     gene            5168..>5829
                     /locus_tag="PLH31_01400"
     CDS             5168..>5829
                     /locus_tag="PLH31_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR87936.1"
                     /translation="MIAFEAASKTYAPKANAKGGGHAALSEVSLSVQAGEVFGVIGAS
                     GAGKSTLIRLINGLEKPTGGRVVVDGDDVAALGVEGLRALRRRVGMIFQHFNLLSSKT
                     VADNVAFPLKLAGRPAVEVKARTAELLARVGLSDQATKYPAQLSGGQKQRVGIARALA
                     TGPKILLCDEATSALDPETTEQILDLVSNLNRELGLTIVLITHEMDVVRRVCDRVAVL
                     EAG"
BASE COUNT          950 a         1921 c         2002 g          946 t
ORIGIN      
        1 cgaagccggt gggttatgcg gacgccttcg atggtgacgt cgcaggcgta gactaggatc
       61 tcgacgccgc ccgcgtgcgc ctgcgccaag cctcgcgcga acgccgggtc caaatccgcg
      121 caggccttga agctttcgca gtcttcccgt tgcaccacaa acagcgccac cgcccgatct
      181 ccagcggaga cctgagccgc taggtccgcc agatgccgcg tcgacctagc cgctacgcaa
      241 tcgggaaact ccgccaggcc cggcgtgcgc gacaggtgca cgttcttcac ctccagccaa
      301 cagtgcgccc ggtccggtcc ggtcagcaga aagtccacgc ggctggcgtc ggcgtacttc
      361 acctccggcc gcaaggtcgc gtagccggaa agctcgggga tggcgtcggc ggccagggcc
      421 tcggcgacca gcttgttggg cagcatggtg ttgacgccga ccaatacgct cccttgggga
      481 cccccgttcg cctccaccag ttgcagcgtc cagggcagtt tgcgcttggg atcgtccgac
      541 cacgacaccc aggcgccctg tcctggcagg ttcacgccca gcatcgcgcc ggggttgggg
      601 cagtgggcgg tgatcgcccg gccgtcatcc aggacgatgt cggcgaagaa gcgcttgtag
      661 cgttgcacaa gtcggccgcg aacgagcggt tgcgggagca gcatggcggc cctaagtcgg
      721 attggagaac agacaggcga tgtaggggag cgcggcgatg acggccagca aggatcgggt
      781 gacggcggcg gtgctgatca tcggcgacga gattctttcg gggcggaccc aggacgtcaa
      841 tctcagcgcc atcgccaaat atctggccac ctatggggtc gacctgtccg aggctcgggt
      901 cgtgcccgat gtcgagcagg agatcatcga cgcggtgaac gccctgcgca ccaagtatga
      961 ctatgtgatc accaccggcg gcattggtcc cactcacgac gacatcaccg ctgattctgt
     1021 ggccaaggcc tttggcgtta ccgcgcccga gcatcccgag atcatggccc tgctgcgcga
     1081 acgctggggt gagcccaacg ccgcccgccg ccgcatggcc cacgtgcctg agggcggggt
     1141 gctggtgaag aacccggtgc aggggccccc aggattccag atcgagaacg tcttcgtgct
     1201 ggcgggcgtg ccggcgatca tgcgcggcat gctgggcgat gtcggcccgc gtctgcgcac
     1261 cggggcggtg gtgatcagca ggactgtccg cgtcaccggc tcgggcgaag gcctgatcgc
     1321 cgcgccgctg gaggcggtgg ccaaggccca tccggacatg tcgctgggca gctatccgtt
     1381 cttctcgccg cccgacatct atggcgccaa cctggtcgtg cgcgggcgtg atccggccga
     1441 ggtcgatacg gcggtcgagg aactggtcgt ggcgctgacg gaggccggcg cggcccagat
     1501 cgagaggatc gcgccggacg cctgagttcc gctcacaaga cgtcagcttc gcagcgcgac
     1561 gtccccagca cgggtcatgt tcgcggaaaa tggtgttggg ccgcacagac gtcgcgagtc
     1621 cttaagatac ccgtgctagt tgaccgtgag cctcggggac gaggcgcgtg aaggtggggg
     1681 ctttgtgagc gcgatctatc cggtgatcct gtgcggcggc tcgggtacgc ggctttggcc
     1741 ggcgtctcgc gcggatcagc ccaaacagtt cctcaaactg attggtgaga cctccagctt
     1801 ccaggatacc gtcctgcgcg tgaaggatct gcccggccgg gccgagatcg tcatcgtcac
     1861 gggcggggcc atggcctcgt tcgtgcgaga ccagaccgcc cagatcggcg tcgaggtcac
     1921 ggtgctgatc gagcccgagg cgcgtgattc cgcgccggct gtggcggcgg cggccgccta
     1981 tgtgcagtcg cgtgatccgg agggcgtggc cctgatgctg gccgccgacc actacatcgc
     2041 cgagcccgag atcttcggcc aggccgccgt gaccgccgcc aaggcggcca gccaaggctg
     2101 gatcgtcacc tttggcgtca agccgaccgg accggcgacg ggctttggct atatccgccc
     2161 cggcgcggct ctgctggacg gcagcgtcaa cgccgtcgcc gcctttgtcg aaaagcccga
     2221 ccgcgccacc gccgagcgct atgtggccga cggctatctg tggaacagcg gcaatttcgc
     2281 cttcaaggcc ggnnnnnnnn nnccgcgttc tccggcgccg ccaagatctc gctcgactac
     2341 gccgtcatgg agcgcaccca gaaggccgcc gtggtccccg ccgccttctc gtggtcggac
     2401 ctgggcgcct gggatgcgat ctgggacgca gcggcccgtg acgaggccgg caacgccgtc
     2461 tttggcgacg ggaccctgat cgacagccgc aacgtgctga tccgctcgac cggcccgttc
     2521 gtcggggtga tcggctgcga ggacctggtc gtggtggccg agcccgacgc catcctggtc
     2581 tgtcgccgcg acgccagcca gtcggtcaag accctggtcg acggcctcaa gcgcgatggc
     2641 cgctccatcg ccagcggccg tgcgcgcgaa ggcgcgccac gacgcgttct ggccacgacc
     2701 gccggcgccc gggtggaaca gtggaccctg aaggccagcg agacggtcga gctgcccatc
     2761 ggtacggttc aggtgctgga aggcgccgtc gagggcctga acgcgctggc ggccatcgac
     2821 gccgtcacgc aagtcaccgc cagcgtcgcc accgtccttc tggtgacgcg caagctttga
     2881 ggcgctgaag cgtttccaac gcgaggtggt gcgggcggcc ggaatcgaac cggcatagcc
     2941 tttcggctga cggattttct taccacttcg gctttcgccg ccgcccagag ggtcgttcgt
     3001 ggtctggact atcccttcac cgtactccgg cgagccggag gttaggtgct gcccgtctag
     3061 tctctacacc ttccctcgaa gagggcttgg ctcgggatcg ccatctgaca gggttccccg
     3121 aatttgagca gttctacatc ccggatttcc ccgggcgcac tcaatcgtct ggttcaagtc
     3181 cgttgcgtct accagttccg ccacgcccgc ctaaaccgag ccgagctata atcgggcgag
     3241 ccgaagacgt cgaggctttc gaggcgcgac ggtgcgtggc gtgctagcct tgagttgacc
     3301 aggatgaaag cccgcatcgc gatgccgcga agcgtccggc gcgtcctgcc ctggagaccg
     3361 ccttgcgccg catactgatc ctgagcctcc tgcttgtcgc cggctgcgcc ccgtccagtc
     3421 catccggctt aaccccggtg actgccgccg cgacgccggt tgtcctggcg gatccggcgg
     3481 cccgcgggcg tgtgctggtc gaggcgcgct gcgcgacctg tcacgcggtg gaggctgtcg
     3541 gcgacagccc cgtggcggca gcgccacctc tgcgcaccct gggtcagcgc tatcccgtcg
     3601 ccaatctgca ggaagcgttc gccgagggca tctcgacggg tcacccgcag atgccgcagt
     3661 tcgttctgga ggcgggtcag gtgcaggatc tgatcgccta tctggaaagc atccagatcc
     3721 ccgccaggaa gtcctgatcc aggctcgacc gtacgcgatc ccatgctagc gtgaccgttc
     3781 tgaatcagga cggggatgaa catgcgggtt ggtatcaagg ctgtggcgat cgcggcggcg
     3841 ttttgcgcgg cgggctcggc tcaggcggcg gagatcacgg ccgtgagcgc agcgcggctg
     3901 ctggacgtgg cgtcgggcaa atatgtcgac aatccgatgg tgatcatcac cgacggtcgc
     3961 atcaccgcgg tgggcaagca gggcgaggtc gccgtgccgg cgggggccaa ggtcaccgac
     4021 ctgccgggcg cgaccctgct cccgggtctg atcgatatgc acgtccacat cgacagcctg
     4081 gccgaggtgg gcggctataa cagcctgcaa tatagcgacg cgttctggag cgtggtccag
     4141 acggccaacg ccagggccac ggtcgaggcc ggcttcacca ccatccgcaa cctgggcgcg
     4201 gaccgcttca acgatgtggg cctgcgcgag gcgatcgagg cgggctatgt gccgggaccg
     4261 cgcatcgttc cggccgccta ttcgatcggc gccaccggcg ggcattgcga ttccacctac
     4321 ttcccgccct cgatggctca gaaaggccct tatgtggccg acggtcccga acagggccgg
     4381 cagaaggtgc gcgaggtgcg caagtatggc gcgcgggtga tcaagatctg cgccaccggc
     4441 ggggtgttct cgcgcaacac cgagccaggc cagcagcaac tgtccctggc cgagatgacc
     4501 gccatcgtcg acgaggcgca catgtggggc ctgaaggtcg ccgcccacgc ccacggcgcg
     4561 gcgggcatca aggacgccat ccgcgccggc gtcgacacca tcgaacatgt cagcctggtg
     4621 gatgacgagg gcatcaagct ggccgtccag aagggagcct ggttctcgat ggacatctac
     4681 aacaccgact acacccaggc cgaaggcaag aagaacggcg tgctggaaga caacctgcgc
     4741 aaggaccgcg agatcggcga cgtgcagcgc gaaaacttcc gcaaggcggt caaggccggt
     4801 gtgaagatgg tgttgggcac cgacgccggc atctatccgc acggtcagaa cgccaaacag
     4861 ttcgcggtga tggtgcgcta tggcgccacc ccgctgcagg ccatccaggc cgccacggtc
     4921 aacgccgccc aggcgctggg ccaggagaag gacgtgggcc aggtggcggt gggtcgctat
     4981 ggcgacatca tcgccgtgac cggcgatcct ctggccgacg tgaccaccct ggaaaagccg
     5041 gtcttcgtga tgaagggcgg ggcggtggtg cgccagccct gagacacctc tgggaaaacg
     5101 gcgtcaggat ggctagttac gggccaacgc tccgccatgg agccgccagc ccgggatgcc
     5161 tgacgccgtg atcgccttcg aggccgcttc caagacctat gcccccaagg caaatgcaaa
     5221 gggtggcggc cacgccgctc tgagcgaggt gtccctgtcg gttcaggccg gcgaggtgtt
     5281 cggcgtgatc ggggcttcgg gcgccggcaa gtccaccctg atccggctga tcaacggcct
     5341 ggaaaagccg accggcggcc gcgtcgtggt ggatggcgac gatgtcgccg ccctgggcgt
     5401 cgagggcctg cgcgcccttc gccgccgggt gggcatgatc ttccagcact tcaacctgct
     5461 gtcgtccaag accgtggccg acaatgtggc ctttccactg aagctggccg ggcgtcccgc
     5521 cgtcgaggtc aaggcgcgca cggccgagct gctggcgcgg gtggggctgt cggaccaggc
     5581 cactaaatat ccggcccagc tgtcgggcgg ccagaagcag cgggtgggca tcgcccgggc
     5641 cctggcgacc ggtcccaaga tcctgctgtg cgacgaggcg accagcgcgc tggatccgga
     5701 aaccaccgag cagatcctcg acttggtttc caacctcaat cgcgaactgg gcctgaccat
     5761 cgtgctgatc acccacgaga tggacgtggt tcgccgcgtc tgtgaccggg tggcggtgct
     5821 ggaggccgg
//
LOCUS       DAOMEO010000033         5786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1838_length_5786_cov_2.815564, whole genome shotgun sequence.
ACCESSION   DAOMEO010000033 DAOMEO010000000
VERSION     DAOMEO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_5786_cov_2.815564"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1600)
                     /locus_tag="PLH31_01405"
     CDS             complement(83..1600)
                     /locus_tag="PLH31_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR87937.1"
                     /translation="MADGGLKSLTIVGGGTAGWMSAAMLSRALGSTVAITVVESDEIG
                     TVGVGEATIPAIKLLNTFLGLDENDFLATTNGTIKLGIEFVDWHTVGQSYLHAFGPVG
                     RPLGLAAFHHYWNRRRLQGHDESLWDYSLNALAARAGRFDRLQTAGGGYLEGLAHAFH
                     FDAALYAQYLRRYAEAQGVRRVEGKIVGVSQHPETGFVTGLSLQDGRNVGGEFFIDCS
                     GFRGLLIEETLKTGYDDWRSWLPMDSAIAVPCASVTPLTPFTRATAREAGWQWRIPLQ
                     HRTGNGYVFCSDYIDAAQATDVLMRNLDGAPLADPRQLTFTTGRRKRFWNRNVVAMGL
                     ASGFMEPLESTSIHLVQSALSRLIALFPNADFNTVEIDEYNRQTALEYEYIRDFLVLH
                     YKATTREDTPFWRACKAMEIPDTLKARIELFAQSGRIFSKEDDLFKEASWVQVLIGQG
                     VLPGAAHPLTGVVTDQQLDEYMANIRQIMGRAVEALPDHADFIARQCAAASRPAA"
     gene            complement(1698..4469)
                     /locus_tag="PLH31_01410"
     CDS             complement(1698..4469)
                     /locus_tag="PLH31_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87938.1"
                     /translation="MNSRTQRSRARLMGGAATSLALLFASAGLAQAQETTTNTAEQDS
                     AVEEIVVTGIRASIENAIALKKTSTSIVEAISAEDIGKLPDNSIAESLARLPGLTAQR
                     LDGRAQVISIRGLGPDFTSTLLNGREQVSTGDNRGVEFDQYPSEILSGVVVYKTPDAA
                     LIGQGLSGTADLQTIRPLKYGKRILSANARYEMNSEDKLNPDAKDTGHRVSATYVNQF
                     ADDTIGVAVAVSDISTPTQSRRFNAWGYPTINAANDRVIGGAKPYNQSNNLKRTGVIG
                     VLEFKPNDKLSTSLDLYYSEFAEEQILRGIELPLYWSSAMLQPGATVQNGLVTQGTYT
                     GVKGVMRNDLNTRDTTLKSAGWNINYQTDNGWGLIADLSYSSADRTDVIFESYSGTGP
                     NGVGATDTLGFTTTPGAGTVFQSTLDYTNRNLFMLTDPQGWGGGAAGGALTQAGFYNT
                     PSITDELSAARFSAKHDLAWGPVTSVEFGVNASRREKEKNVNESFLTFGGRIADGAPL
                     SRAIPEAAIVGVAKLDMIGISGMLAYDPTYLLKNGVYTLIADQNPAVQTRNWSVREDV
                     QVAYAKFGVDTQAGAIPVRGNFGLQVVRTDQFSSGVAVNPNDTANPTVTDDGDTYTYA
                     LPSLNLTFDMGNELYVRVGAARTLARARMDELRASQSFNQNPTRLTSTDPNTSYFSAN
                     GGNPKLRPYIADGVDLSVEKYFGRSGYISGAVYHKELSNFVNSSSSSLKDFSDFLFVL
                     SPAQRAALGTPLGLVSGPDNGDGGYIRGIELSASVQGDMLWQPLKNFGLIVSGSFTDS
                     EVTLEKGQDPITIPGLSKKVVNTTLYYENNGFNARVSNRYRGKFLGEVAGLSAARVFR
                     TVEAESVLDAQIGYEFRDGPLNGMALLLQANNITNEPFKTYDNNDSRQTIDYQEYGAT
                     YMIGVSYRY"
     gene            4782..>5786
                     /locus_tag="PLH31_01415"
     CDS             4782..>5786
                     /locus_tag="PLH31_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQR87939.1"
                     /translation="MSAWGSWRQGLMAMAAGVAMVAVGGVACAAPGSAAYLQRKPADE
                     VIYFVLPDRFANGDAVNDRGGLTGGPLVTGFDPRRAGFYHGGDLKGLTGKLDYIQGLG
                     ATAVWLAPIFRNKPVQGAPGRESAAHHGYWITDFTDVDPHFGTRADFKALVDAAHARG
                     MKVYMDIITNHTADVIQYRECPANDCGYRSKADYPYATRGGVGGAAINDGFDGRDFTR
                     LTRPDYAYTPYVPAGEERVKTPAWLNDLAHYHNRGDSTFAGESSLHGDFAGLDDLFTE
                     NPRVVQGFIDIYGQWIDDFGIDGYRIDTARHVAPAFWQAFIPAMQARAKARGIDNFHI
                     F"
BASE COUNT          960 a         1767 c         2000 g         1059 t
ORIGIN      
        1 gccgcagtga cgcctggcgg caaaaagaac gcctccgcca ggcaggtccg gcggaggcgt
       61 caggggcgtt tggaaaacac agtcaggcgg cggggcgtga agccgctgcg cattgccggg
      121 cgatgaagtc ggcgtggtcg ggcagggcct cgaccgcgcg acccatgatc tggcggatgt
      181 tggccatgta ctcgtcgagc tgctggtcgg tgacgacgcc ggtcaacgga tgcgccgcgc
      241 cgggcagcac gccctggccg atcagcacct ggacccagct ggcctccttg aagaggtcgt
      301 cttccttgct gaagatccgg cccgattggg caaatagctc gatgcgagcc ttcagggtgt
      361 cggggatctc catggccttg caggcccgcc agaacggcgt gtcctcgcgg gtggtggcct
      421 tgtagtgcaa gaccaagaag tcgcggatgt actcgtattc cagcgcggtc tgacgattgt
      481 actcgtcgat ctcgacggtg ttgaagtcgg cgttggggaa cagggcgatc aggcggctga
      541 gggcagattg caccaggtgg atactggtcg actccagcgg ctccatgaag ccgctggcca
      601 gacccatggc cacgacgttc ctgttccaga accgcttgcg cctgccggtt gtgaacgtca
      661 gctgccgggg atcggccagg ggcgcgccat cgagattgcg catcagcacg tcggtcgcct
      721 gggccgcgtc gatatagtcg ctgcagaaca catagccgtt gccggtgcgg tgctgcagcg
      781 ggatgcgcca ctgccagccg gcctcgcgag ccgtggcgcg ggtgaagggc gtcagcggcg
      841 taaccgaggc gcagggcacg gcgatggcgc tgtccatcgg cagccagctg cgccagtcgt
      901 catagccggt cttcagcgtc tcctcgatca gcaggccgcg aaaacccgag cagtcgatga
      961 agaactcccc gcccacgtta cggccatcct gcaggctcag gccggtgacg aacccggttt
     1021 cgggatgctg gctgacgccg acgatcttgc cctccacgcg gcgaacgccc tgggcctcgg
     1081 catagcggcg caggtactgg gcatagagcg ccgcgtcaaa gtggaaggcg tgggccagcc
     1141 cctccagata gccgccgccc gccgtctgca ggcggtcgaa cctgccggcg cgggcggcca
     1201 gggcgttcag cgaatagtcc cacaggcttt cgtcatggcc ctgcagccgg cggcggttcc
     1261 agtagtgatg gaaggccgcc aggcccagcg gacgcccgac ggggccgaag gcgtgcaggt
     1321 aggactggcc cacggtgtgc cagtcgacga actcgatacc cagcttgatc gtgccgttgg
     1381 tagtcgccag gaagtcgttc tcgtccaggc ccaggaaggt gttcagcagc ttgatcgccg
     1441 ggatggtggc ctcgccgacg cccaccgtgc cgatctcgtc ggactcgacc accgtgatcg
     1501 ccacggtcga gcccagggcg cgggagagca tggcggcgct catccagccg gccgttccgc
     1561 cgccgacaat ggtcagggac ttcagtccgc cgtccgccat cactgctccg cccggtttgc
     1621 ccctgatggg gtctctcata aaaaagcccc cgccagacag gtctggcggg ggctggtagt
     1681 tggggatctt ggaccgccta gtagcggtag gaaacaccga tcatgtaggt cgcgccgtac
     1741 tcctgatagt cgatggtctg gcgcgagtcg ttgttgtcat aggtcttgaa gggctcgttg
     1801 gtgatgttgt tggcctgcag cagcagggcc atgccattga gcgggccgtc gcggaactca
     1861 tagccgatct gggcgtccag caccgactcc gcctccaccg tacggaacac ccgcgcggcg
     1921 ctgaggccgg cgacttcacc caggaacttg ccgcgatagc ggttgctgac ccgggcgttg
     1981 aagccgttgt tctcataata gagggtcgtg ttgacgacct tcttggacag gcccggaatg
     2041 gtgatcggat cctggccctt ttccagcgtg acttcgctgt cggtgaacga gccgctgacg
     2101 atcagaccaa agttcttgag cggctgccac agcatgtcgc cctggaccga ggccgacagt
     2161 tcgatgccgc ggatatagcc cccgtcacca ttgtccggac cggaaaccag gcccagcggc
     2221 gtgccgagcg cggccctctg ggcggggctg agcacgaaca ggaagtccga gaagtccttc
     2281 aggcttgacg agctggagtt gacgaagttc gacagctcct tgtggtagac cgctccggag
     2341 atgtagcccg agcgaccgaa gtacttctcg accgacagat cgacgccgtc ggcgatatag
     2401 ggccgaagct tgggattgcc gccattggcg ctgaagtagg acgtgttcgg atcggtggag
     2461 gtcagacgcg tcgggttctg gttgaaggac tggctggcgc gcagttcgtc catccgggcg
     2521 cgggccaggg tgcgcgccgc gccaacgcgg acgtacagct cattgcccat gtcgaaggtc
     2581 aggttcaggc tgggcagggc gtaggtatag gtgtcgccat cgtcggtcac ggtcgggttg
     2641 gccgtgtcgt tcggattgac ggcgacgccg ctcgagaact gatcggtgcg aaccacctgc
     2701 aggccgaaat tgccgcgaac cgggatggcg ccggcctggg tgtcgacgcc gaacttggcg
     2761 taggcgacct ggacatcttc gcgaaccgac cagttgcggg tctgcacggc cgggttctgg
     2821 tcggcgatca gggtatagac gccattcttc agcagatagg tcggatcata ggccagcatg
     2881 ccggagatgc cgatcatgtc cagcttggcg acgccgacga tggcggcttc cggaatggcc
     2941 cggctcaggg gcgcgccgtc ggcgatgcgg ccaccgaagg tcaggaagct ctcgttgacg
     3001 ttcttttcct tctcccggcg gctggcgttg acgccgaact cgacgctggt gaccggaccc
     3061 caggccaggt catgcttggc cgagaagcgg gccgccgaca gctcgtcggt gatcgaaggg
     3121 gtgttgtaga agccggcctg ggtcagggcg ccgccggcgg cgccgccgcc ccaaccctgc
     3181 gggtcggtca gcatgaacag gttgcggttg gtgtagtcca gcgtcgactg gaacaccgtg
     3241 cccgcgccag gcgtggtggt gaagcccagg gtgtcggtgg cgccgacgcc gttggggccg
     3301 gtgcccgagt aggactcgaa gatcacgtca gtgcgatctg ccgacgagta gctgaggtcg
     3361 gcgatcaggc cccagccgtt gtcggtctgg tagttgatgt tccagccggc tgatttcagg
     3421 gtggtgtcgc gggtgttgag gtcgttgcgc atcacgccct tgacgcccgt ataggtcccc
     3481 tgggtgacca ggccgttctg cacggtggcg ccgggttgca gcatggccga cgaccagtac
     3541 agcggaagct cgatgccgcg caggatctgc tcttcggcga actccgaata atacaggtcc
     3601 aacgaggtgc tgagcttgtc gttgggcttg aactccagca cgccgatcac gccggtgcgc
     3661 ttgaggttgt tggactggtt atagggcttg gcgccgccga tcacgcgatc gttggccgcg
     3721 ttgatggtcg gatagcccca ggcgttgaag cgacggctct gggtcggggt cgagatatcc
     3781 gaaacggcca cggccacgcc gatggtgtcg tcggcgaact ggttgacata ggtggcgctg
     3841 acgcggtggc cggtgtcctt ggcgtccggg ttcagcttgt cttcgctgtt catctcatag
     3901 cgggcgttcg ccgagaggat gcgcttgccg tacttcagcg ggcggatggt ctgcaggtcg
     3961 gcggtgcccg agaggccctg gccgatcagc gcggcgtccg gggtcttgta gaccacgacg
     4021 cccgacagga tttccgacgg atactggtcg aattcaacgc cgcggttgtc gccggtgctg
     4081 acctgctcgc ggccgttcag cagcgtggag gtgaagtcgg ggccaaggcc gcggatcgag
     4141 atcacctggg cgcggccatc aaggcgctgg gcggtcaggc cgggcaggcg ggccagggat
     4201 tcggcgatcg aattgtcggg cagcttgccg atgtcctcgg ccgagatggc ttcgacgatc
     4261 gaggtcgagg tcttcttgag cgcgatggcg ttttcgatgc tggcgcgaat gccggtgacc
     4321 acgatctcct cgacggccga atcctgttcg gcggtgttgg tggtggtttc ctgggcctgg
     4381 gccaggccgg ccgaggcgaa cagaagcgcc aggctggtcg ctgcgccgcc catcagacgc
     4441 gcacggctgc gctgagttct tgagttcatg gtctcaccct ccctgtatcg gctctgcgcg
     4501 tttctgacgc tgcggtcgga gccgttgtcg caaattactg caaattaaca cgatgtaatc
     4561 aacccaaaaa aacgcaaagc ctgtcacgca gcaagcccat cagcctcgtt tgccgggctg
     4621 actgatgcaa atttgctgcg gataagcccc ggcccgcatg gatccgcgca ggtagatggc
     4681 aggcttgcga ttgacagccg cgcacgctgc ctgcaaattt atgcaaatta ttgcaacgga
     4741 tgacgccggt ctcggtgcgt tcgtgcggct agggggaaac gatgtcggcc tgggggtcct
     4801 ggcgacaagg gctgatggcc atggcggccg gcgtcgccat ggtggcggtc ggcggcgtgg
     4861 cctgtgccgc gcccggctcc gcggcctatc ttcagcgcaa gccggccgat gaggtgatct
     4921 acttcgtgtt gcccgaccgg ttcgccaatg gcgatgcggt caatgatcgc ggcggcctga
     4981 cgggcggtcc gctggtcacc ggctttgatc cccgccgggc cggcttctat catggcggcg
     5041 atctcaaggg cctgaccggc aagctggact atatccaggg tctgggcgcg accgccgtct
     5101 ggctggcgcc gatcttccgc aacaagccgg tgcagggtgc gccaggacgc gagtcggcgg
     5161 cccatcacgg ctactggatc accgacttca ccgacgtgga tccgcatttc ggcacgcggg
     5221 cggacttcaa ggccttggtc gacgccgccc atgcgcgcgg gatgaaggtc tatatggaca
     5281 tcatcaccaa ccataccgcc gacgtgatcc agtatcgcga gtgtccggcc aacgactgcg
     5341 gctaccgcag caaggccgac tacccctatg cgacacgcgg cggggtaggg ggcgcagcga
     5401 tcaatgacgg cttcgacggt cgcgatttca ccaggctgac ccggcctgac tacgcctaca
     5461 cgccctacgt tccggctggc gaggagaggg tcaagacgcc cgcctggctg aacgatctgg
     5521 cccactatca caatcgcggt gacagcactt ttgccggcga aagctcgctg cacggcgact
     5581 tcgccgggct ggacgaccta ttcaccgaga accctcgcgt ggtgcagggc ttcatcgaca
     5641 tctatggcca gtggatcgac gatttcggca tcgacggcta ccgcatcgac accgcccgcc
     5701 atgtggcgcc ggcgttctgg caggccttca ttccggccat gcaggcgcgc gccaaggccc
     5761 ggggcatcga caacttccac atcttt
//
LOCUS       DAOMEO010000034         5749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1849_length_5749_cov_2.428872, whole genome shotgun sequence.
ACCESSION   DAOMEO010000034 DAOMEO010000000
VERSION     DAOMEO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_5749_cov_2.428872"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLH31_01420"
     CDS             complement(<1..1215)
                     /locus_tag="PLH31_01420"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQR87940.1"
                     /translation="MADIASLQERPLTLDELTAYFASGSKPREQFRVGAEHEKFGFYL
                     NSHAPVPYYGDNGVHALLTGLQRFGWKPVMEGEVVIGLERNGANVSLEPGGQFELSGA
                     PLLTMHDICAETGQHLDEVKTVADELGLGFMGLGFSPVWKREDVPVMPKGRYVIMRNY
                     MPKVGGLGLDMMLRTCTVQANLDFSSEADMVAKFRMSLALQPIVTALFANSPFTEGKP
                     NGFLSARANVWTDTDPDRTGLLDFVFEDGFDFERYARYALDVPMYFAKRGDRYIDLAG
                     RSFRDFIDGRIDELPGERASLKDWADHTTTLFPEVRLKTYLEMRGADAGPWSSLCALP
                     ALWTGLFYDDAALAAAWDLCKHWTPEDRAGLRRDVPVLGLKAPIAGRTTQDVARDLVA
                     IAKSGLKNRAHVN"
     gene            complement(1334..2062)
                     /locus_tag="PLH31_01425"
     CDS             complement(1334..2062)
                     /locus_tag="PLH31_01425"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQR87941.1"
                     /translation="MIRLFVPQDLAPGYGVVLSLDQSRYLTSVMRQAIGAQVLLFNGR
                     DGEWTATLTETGKRGSLLKVDAQVRPMALGPDLDLVIALVKRGRVETIVEKAAELGVR
                     RVRLTLTRRTNVDFVKLGRLDAIAMEAAEQTGRLDVPTVEDPVKLAEILDGWDPARKL
                     VFCDEGGEALPAFQALAGSAAPAAILIGPEGGFSPEERERLRSLPFVTPVSLGPRILR
                     ADTAAISALTLWQAAAGDWRENES"
     gene            2208..2969
                     /gene="ppk2"
                     /locus_tag="PLH31_01430"
     CDS             2208..2969
                     /gene="ppk2"
                     /locus_tag="PLH31_01430"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQR87942.1"
                     /translation="MSKSDDYEAELARLQLALIGMQKKAIAEGWQILAVFEGRDAAGK
                     DGAISRITEHLDRRQTIAVALPKPSDRERSEWYFQRYVEWLPACGEAVLFNRSWYNRA
                     GVEKVMGFSTPQQQEQFLRDVPAFEKMLVENGMRYAKFWLDITRETQAERLKARSADP
                     LKAFKISPLDAVAQEKWDDYTDARDDMLTRTSTTEAPWICVRADHKKAARLNIIRWLL
                     HASGDKTLLKGVAQPDPEVIFPFDVAALTDGRLAR"
     gene            complement(3134..3514)
                     /locus_tag="PLH31_01435"
     CDS             complement(3134..3514)
                     /locus_tag="PLH31_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQR87943.1"
                     /translation="MKAAAASIDAYLADLPVAQRTALEVLRRQLLAAAPGAEECISYG
                     LPTFKLNGNLVHFGAAARHCAFYPGAVVDRFAARLAGFETSKGTIRFQPEKPLPPDLV
                     REIVEHCVARNAEIAAGRAARKRK"
     assembly_gap    3817..3861
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3862..>4633
                     /locus_tag="PLH31_01440"
     CDS             <3862..>4633
                     /locus_tag="PLH31_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HQR87944.1"
                     /translation="VLAALAVPFVILAGIGALRRNSLIVWALGAAALAAYLAFHGVWR
                     ETTPDKMPDVPVFLAIAGGLFIAHHLIQPAQAERRWVARYPAYFDVTWMHGVQLALSA
                     AFTGVFWILLYLGASLFKLIGIEAIQKLISEEAFAFPATTVMFAAAVQLTDVRSTLVR
                     GVRTVALTLLAWLLPLMTLIAAGFLASLPFTGLEPLWKTRAATALLLTAAAHLIVLIN
                     AAYQDGEDAPHLILRWAARIAGVLLVPIGLSCRIYPTTN"
     assembly_gap    4634..4648
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4649..5485
                     /locus_tag="PLH31_01445"
     CDS             <4649..5485
                     /locus_tag="PLH31_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HQR87945.1"
                     /translation="GLALRIGQYGLTPERVFAAAFVLVALGHAAGYAMAALRRDVWMK
                     TLERTNVVMAFVSVALILALFTPLADPARLSVSDQVARLERGKVKPAEFDYAFLRFDG
                     GRYGRAVLTRLKTDKNPEIAKGAAQALAWTARPPLDARPRFTKTTVYPTGRVLPESLV
                     KQDWSLSEGDSVCVYPDNPCSALLLDVDGDGKEEVLFANGGTLDVIKQFPKDSWKKIG
                     TASARCSGTDLDQALKSGTARMAEPVPRQDILVGDLRLGFTPFAAGCAVEPELESVLT
                     RK"
     gene            complement(5618..>5749)
                     /locus_tag="PLH31_01450"
     CDS             complement(5618..>5749)
                     /locus_tag="PLH31_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87946.1"
                     /translation="TVRGADRILVFQQGRVVEEGSHAELKARGGVYARLNAVSEGVG"
BASE COUNT          913 a         1886 c         1948 g          942 t
ORIGIN      
        1 gttcacgtgg gcgcggttct tcaggccgga cttggcgatg gcgacgaggt cgcgggccac
       61 gtcctgggtg gtgcggccgg cgatcggggc cttcaggccc agaaccggca catcgcggcg
      121 caggcccgcg cgatcctcgg gcgtccagtg cttgcagaga tcccaggcgg cggccagggc
      181 ggcgtcgtca tagaacaggc ccgtccacag ggccggcagg gcgcacaggc tgctccatgg
      241 gccagcatcg gcgccgcgca tttccagata ggtcttcagg cgtacttcgg ggaacagggt
      301 ggtggtgtgg tcggcccagt ccttgagcga ggcgcgctcg ccgggcagct cgtcgatcct
      361 gccatcgatg aagtcacgga agctgcggcc ggccaggtcg atatagcggt cgccgcgctt
      421 ggcgaaatac atcggcacgt ccagggcgta gcgggcgtag cgctcgaaat cgaagccgtc
      481 ctcgaacacg aaatccagca ggccggtgcg atcggggtcg gtgtcggtcc agacgttggc
      541 gcgcgcggag aggaagccgt tgggtttacc ttccgtgaac ggagaattgg cgaacagggc
      601 cgtgacgatg ggttgcaggg ccaggctcat gcggaacttg gcgaccatgt cggcttcgct
      661 ggagaagtcg agattggcct gcaccgtgca ggtgcgcagc atcatgtcga ggcccaggcc
      721 cccgaccttg ggcatgtagt tgcgcatgat gacatagcgg cccttgggca tcaccggcac
      781 gtcttcgcgc ttccacacgg gcgagaagcc caggcccatg aagcccaggc ccagctcgtc
      841 ggcgacggtc ttgacctcgt ccaggtgctg gccggtctcg gcgcagatgt cgtgcatggt
      901 cagaagcggc gcgccggaca gctcgaactg accgccgggc tccaggctga cattggcgcc
      961 gttacgctcc agcccgatga cgacctcgcc ttccatcacc ggcttccagc cgaagcgctg
     1021 aaggccagtc agcagggcgt ggacgccgtt gtcgccgtaa tagggaacgg gcgcgtggct
     1081 gttcagatag aagccgaact tctcgtgttc tgccccgacg cggaactgct ccctgggctt
     1141 ggagcccgag gcgaaatagg cggtcagttc gtcgagcgtc agcggacgct cctgcaggct
     1201 agcgatgtcg gccatgagcc gtctctcccc aaggtctcat acgcattgag tagggagagg
     1261 ccctcccccc gaccagccgc gatcttgtaa cgcgcaattg ggcgttttct ttgtgaacct
     1321 caagaggcga agcctagctt tcgttctcgc gccagtcgcc ggcggcggcc tgccagaggg
     1381 tcagggcgct aatggcggcg gtgtcggcgc gcaggatgcg ggggcccagt gacaccggcg
     1441 tgacgaaggg caaagaccgc aggcgctcgc gttcctcagg cgaaaagccg ccctcgggcc
     1501 cgatcaggat ggcggccggc gcggcgcttc ccgccagggc ctggaaggcc ggaagcgcct
     1561 cgccaccctc gtcgcagaac accagcttgc gggccggatc ccagccgtcg aggatctcgg
     1621 ccagcttgac cgggtcttcc acggtcggca cgtccaggcg gccggtctgc tcggcggcct
     1681 ccatggcgat ggcgtccaga cgccccagct tgacgaagtc gacattggtc cgccgcgtga
     1741 gggtcagccg aacccggcgc acgcccagct cggcggcctt ttcgacgatg gtctcgaccc
     1801 ggccacgctt gaccagggcg atgaccagat caaggtcggg acccagggcc atgggccgga
     1861 cctgcgcgtc caccttcagc agcgagccgc gcttgccggt ttcggtcagg gtggcggtcc
     1921 actcgccgtc gcggccgttg aacagcaaaa cctgggcgcc gatggcctgg cgcatcaccg
     1981 aggtcagata gcgcgactgg tcgaggctga ggacgacgcc atagccgggg gccaggtctt
     2041 gcggaacgaa aagacggatc atgtcgctct tctagcgcgt tccgcaaaag tgtcagcggt
     2101 tttgcggccg gaacgcgcgt caactttatg aatgtagagc cctttttcct ccccattcgg
     2161 ggagcgggct ctaccgcaac ttcactttcc gggggtaggc tcaggtcatg agcaagtcag
     2221 acgactacga agccgaactc gcccgccttc agctggccct gatcgggatg cagaagaagg
     2281 ccatagccga gggctggcag atcctggccg tgttcgaggg ccgcgacgcc gccggcaagg
     2341 acggggcgat ctcgcggatc accgagcatc tggaccgtcg ccagaccatc gccgtcgccc
     2401 tgcccaagcc ctcggaccgc gaacgcagcg agtggtattt ccagcgctat gtggaatggc
     2461 tgccggcctg cggcgaggcg gtgctgttca accgctcgtg gtacaatcgc gccggggtgg
     2521 aaaaggtgat gggcttctcg accccgcagc agcaggagca gttcctgcgc gatgtgccgg
     2581 ccttcgagaa gatgctggtc gagaacggca tgcgctacgc caagttctgg ctggatatca
     2641 cccgtgagac gcaagccgag cgcctgaagg cccgcagcgc cgatcccctg aaggccttca
     2701 agatcagccc gctggacgcc gtggcccagg aaaagtggga cgactatacc gacgcccgcg
     2761 acgacatgct gactcgcacc agcacaaccg aggccccgtg gatctgcgtg cgggccgatc
     2821 acaagaaggc ggcgcggctg aatatcatcc gctggctgct gcatgcgtcg ggcgacaaga
     2881 cgctgctgaa gggcgtggcg caacccgacc ccgaggtgat cttcccgttc gacgtggcgg
     2941 cgctgacgga tggacggctg gcgcggtgac ttccttctcc ccttgcggga gaaggtggcc
     3001 cgaagggccg gatgaggggt cgcgcgaatc tatcaagatc ggcgggctac agggcgcgga
     3061 tggtggatag ggtgttcgac ccctcatccg tcggcctgcg gccgccacct tctcccgcaa
     3121 ggggagaagg atgttacttg cgcttccgag cagcccgtcc ggcggcgatc tcggcgttgc
     3181 gggccacgca gtgctcgacg atctcgcgga ccaggtccgg cggcagaggc ttctcgggct
     3241 ggaaccggat cgtgcccttg ctggtctcaa agccggccag gcgcgcggcg aaccggtcaa
     3301 cgaccgcgcc cggatagaag gcgcagtgcc tggccgcagc gccgaagtgg acgaggtttc
     3361 cgttcagctt gaaggtcggc aggccgtagc tgatgcactc ctccgcgccc ggggcggcgg
     3421 ccaggagctg gcggcgcagg acctccagcg ccgtccgctg agcgacgggc agatcggcga
     3481 gataggcgtc gatggaggcg gcggcggctt tcatggcggt ttcccctgct caatctctcc
     3541 aggacgtcgc cagacggcct catccggcat ccgccgcgat tgagacaaaa agtgcgccca
     3601 catcgccagc ttcacaacgg gcgcgcggcg tggtcctgtc acgcccggcg tcgaggttgc
     3661 gcgaggactt gagcgatgga ccagacgaca gaccgatcgg cgcggaccat caccgcgatc
     3721 cggctgggcg tgggcctgct gcagggcctg gccctgttcc tgctgcatca tgccgagaac
     3781 gtcaaagcct ggcccgccac ccagccgctg gtcttcnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nggtgctggc ggcgctggcc gtgcccttcg tgatcctggc
     3901 cgggatcggg gccttgcgcc gaaacagcct gatcgtctgg gcgctggggg ctgcggcctt
     3961 ggcggcctat ctggccttcc acggcgtgtg gcgtgagacg acacccgaca agatgcccga
     4021 cgtcccggtg ttcctggcca tcgccggcgg cctgttcatc gcccaccacc tgatccagcc
     4081 ggcgcaggcc gagcggcggt gggtggcccg ctatccggcc tatttcgacg tgacctggat
     4141 gcacggggtg caactggccc tgtcggcggc cttcaccggg gtgttctgga tcctgctcta
     4201 tctgggcgcg tcgctattca agctgatcgg catcgaggcc atccagaagc tgatctccga
     4261 agaggccttc gcctttccgg ccaccacggt gatgttcgcc gccgccgtgc agctgaccga
     4321 cgtacgctcg accctggtgc gcggcgtgcg caccgtggcc ctgaccctgc tggcctggct
     4381 gctgccgctg atgaccctga tcgcggcggg ctttctggcc agcctgccgt tcaccggtct
     4441 ggagccgctg tggaagaccc gcgccgccac ggccctgctg ctgaccgccg ccgcccacct
     4501 gatcgtgctg atcaacgccg cctatcagga cggcgaggac gcgccgcacc tgatcctgag
     4561 atgggccgcg cggatcgccg gcgtgctgct cgtccccatc ggactttcgt gccgcatcta
     4621 ccccacaacc aacnnnnnnn nnnnnnnngg cctggccctg cggatcggcc agtatggcct
     4681 gacgccggaa cgggtgttcg ccgccgcctt cgtgctggtc gccttgggcc atgcggcggg
     4741 ctacgccatg gccgccttgc gccgcgacgt ctggatgaag accctggagc ggaccaatgt
     4801 ggtgatggcc ttcgtcagcg tggccctgat cctggccctg ttcacgccat tggccgatcc
     4861 ggcccggctg tcggtgagcg atcaggtcgc gcgtctggag cgcggcaagg tcaagcccgc
     4921 cgagttcgac tatgccttcc tgcgctttga cggcggtcgc tatggccgcg ccgtgctgac
     4981 gcgtttgaag acagacaaga accccgagat cgccaagggc gctgcgcagg ccctggcctg
     5041 gaccgcccgc ccgccgctgg acgcccgccc gcgctttacg aagaccactg tctatcccac
     5101 cggccgggtc ctgccggaga gcctggtcaa gcaggactgg tcgctgtcgg aaggcgactc
     5161 ggtctgcgtc tatcccgaca acccctgctc ggccctgctg ctggacgtgg acggcgacgg
     5221 caaggaggag gtgctgttcg ccaatggcgg gacgctggac gtcatcaagc agttcccgaa
     5281 ggacagctgg aaaaagatcg gcacggcctc ggcccgctgc agcggaaccg acctggacca
     5341 ggcgttgaag tcggggacgg cgcgtatggc cgagccggtt ccgcggcagg atattctggt
     5401 cggggacctg cggctgggtt tcacgccgtt cgcggcgggc tgcgcggtgg aacctgaact
     5461 ggaatccgtg ctgacccgga agtagacacc ccatcaaagc ttgtcatccc gggcgaagcg
     5521 cagcgaagac ccgggaccgc ggcaagcgtc agcgcttccg gcggtcccgg ctcggcgcgc
     5581 ttcgcgcttg gccgggatga caagctattt gaggcgccta ccccacgccc tcgctcaccg
     5641 cattcagccg cgcatagacc ccgcccctgg ccttcaactc ggcatggctg ccctcttcaa
     5701 ccacccgccc ctgctggaac accaggatac ggtcggcccc gcgcacggt
//
LOCUS       DAOMEO010000035         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1866_length_5688_cov_1.813243, whole genome shotgun sequence.
ACCESSION   DAOMEO010000035 DAOMEO010000000
VERSION     DAOMEO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_5688_cov_1.813243"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="PLH31_01455"
     CDS             <1..1542
                     /locus_tag="PLH31_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87947.1"
                     /translation="SLPPGTKAKRADVDFTGADLANNDLRNNIKTFTQELRLVSKSQG
                     PFNWLVGGFYQNEKLATGRTINYGTDIRAFGEGLSGPVPAALLGALPAPLRPALTGRS
                     NLYALEFLQSLVTPSVVPGATYFQAGQGIDDYYTMEQTSFSLFGQADYEVTERLTLTG
                     GLAFLSDKKDAESAVVLRDPFSSLNLSAVPQLAALGLPGNLYSALGGLQFYYGNTTNH
                     APVNFPNATESGRLKDDKVTYAVRAAYDFGAINTYVSYSTGWKAGAFNLSSDSRPPNA
                     NGVGRTAAPEDVSVLEAGLKANFPGGYFNVAVFDQAIEGFQSNAFTGLGYSLVNAGKE
                     SVRGFEVDSAYRPLPWLALTGAVTYLDPKYDSFTGAACVNYDTARCPVDPATGRRPNF
                     RDLTGDKPAGIPKWSLSTSATANHNFGNGYSGYLRVEYDYTSDVQLSETTPPELSTWG
                     QKNVNASLGLLNETHQLEAMLWVRNLTKHESLISTFPTVAQDGSYSGYPSAPRTYGVT
                     LRKTF"
     gene            1819..3639
                     /locus_tag="PLH31_01460"
     CDS             1819..3639
                     /locus_tag="PLH31_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQR87948.1"
                     /translation="MSRPLLFLDPDSDLSLQEQVRRKLVEAAYAGAFPPGKRLPSSRG
                     LATQLGVARITVTLAYQKLLDEGFFVSRERSGIYVDHDMLRGLTAVQSVAGRREPAPS
                     SPWGARFKSSEGDEPVARRSPNWRQYPYPFIDGLFDASLFPVSEWREASRQALALADI
                     SQWSMDGGDADDPMLIEEIRTKVLPRRGIQARPDEILITVGAQQALALISQMLVDRTS
                     VVAVENPGYPDLRRLFMDRGARLVHQPVDDEGMVVDRRLDDCDLVYVTPSHQFPTGVM
                     MTQARREALLKKASARDLIIVEDDFACETNYLDEACPALRSLDRDDRVIYVAGFSKVL
                     GPGLRLGFMVASADVIAEARRLRHLAVRHPPLNNQRTAAHFLAMGHYDATMMRLGRLF
                     RERRTALRDALNHYLQNAVAIAPLRGGATYWVRGPDHLDVEVFAAEAERRGVLIEPVG
                     PYFADSKAPRNIFRLGVTSLPLDRIRQGVAALADLMRDLPGTAHAFPDTASAHLVGAA
                     LQTAMSGAVLLCKTVYGDPCTIELLPNGRMSGRAGYANEDCDEGRWWVEGDFWCRQWS
                     RWSYGETSRLMTTITGDRIGWFDAGGRLVDSAVIRRADLS"
     gene            3782..4657
                     /locus_tag="PLH31_01465"
     CDS             3782..4657
                     /locus_tag="PLH31_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQR87949.1"
                     /translation="MRPIGIAGLQLQLGKGDNGDRIAAEIAAVKARLPWVDMVVLGEL
                     SLFGASISTAQPLPGDAEAKMCAAARASGVWLVPGSLFEADGGRVYNTAPVIDPTGQV
                     VARYRKIYPFLPYEQGVTPGTEFVVFDVPGVGRFGLSICYDMWFPETTRTLVWMGAEV
                     IIHPGMTNTIDREAELAVARASAVTNQVYFVDINTAGQLGVGLSGVFGPGGEVIHQAG
                     SGHEVIAVELDLDQVTRVRERGWHGLGQPLKSLRDNPITFPPYRDGFAASPAFSGLGP
                     LQKPGSEQPGKPRLG"
     assembly_gap    4678..4687
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4717..>5688
                     /locus_tag="PLH31_01470"
     CDS             4717..>5688
                     /locus_tag="PLH31_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR87950.1"
                     /translation="MNKSNLRGAASIGALVMALASAGAGAAQEIRDTTIGEIVVTANR
                     REQSVLEVPYNISAVSGDAIEDGKMLDAAELMRGIPGVSVVDRGYRNGGVVNNVLIRG
                     INVDSNALGDYAVSSVAPVSTYQNDTPLFAGFLLKDLDRVEVLRGPQGTLYGSGSLGG
                     TVRYITRAPRLGQFGGAANITASQVEGSGSTGWAGDLTVNLPLGDMFALRATVSQIDY
                     PGLTDYVNLYVLDSTGAPAAPSGVLSPNAAYTGKKDADTVEISFARVALLFEPSDRFN
                     ATLTYSQQSDEVGGRRQVTRGLDGYGKRYGDYQNGSIQLEPSSRDVSL"
BASE COUNT          982 a         1794 c         1850 g         1052 t
ORIGIN      
        1 tcgctcccgc ccgggaccaa ggcgaaaagg gccgacgtcg acttcaccgg cgccgacctg
       61 gccaacaacg atctgcgcaa caacatcaag accttcaccc aggaactgcg actggtctcc
      121 aaaagccagg ggccgtttaa ctggctggtg ggcggcttct atcagaatga gaagctggcg
      181 acgggccgta caatcaatta cgggaccgac atccgggcct tcggcgaggg gctgagcggt
      241 ccggtgccag cggccctgct gggcgccttg cccgcacctc tgcgccccgc cttgacgggc
      301 aggtccaacc tctatgcgct agaattcctg cagagcctgg tgacgccatc ggtggttccg
      361 ggcgcgacct atttccaggc agggcagggc atcgacgact actacacgat ggagcagact
      421 tcgttctcct tgttcggcca agccgactat gaggtgaccg agcgcctgac cctgaccggc
      481 ggcctggcct tcttgagcga caagaaggac gccgaatctg cagtcgtgct gcgtgacccg
      541 ttctcttcgc tgaacctgtc ggcggtgccg caactggcgg ccctgggtct gccgggcaat
      601 ctctacagcg cgctgggcgg cctgcagttc tactacggca acaccaccaa ccacgcgccg
      661 gtcaacttcc ccaacgctac cgagtcgggg cgcctaaagg acgacaaggt cacctatgcg
      721 gtgcgtgcgg cctatgactt cggcgccatc aacacctatg tcagctactc gaccggctgg
      781 aaggccgggg cgttcaacct gtcgtccgac agccgtccgc cgaacgccaa tggcgtgggc
      841 cgcacggcgg cgcccgagga tgtcagcgtg cttgaggcag gcctgaaggc caatttccca
      901 ggcggctact tcaacgtcgc tgtctttgat caggcgatcg agggcttcca gtccaacgcg
      961 ttcacgggcc tgggctacag cctggtcaac gccggcaagg agtccgttcg cggcttcgag
     1021 gtcgacagcg cctatcgtcc gctgccctgg ctggccctta ccggcgcggt cacctatctg
     1081 gacccaaaat acgactcctt caccggcgcg gcctgcgtga actatgacac cgcccggtgc
     1141 ccggtggatc cggcgaccgg ccggcgtccg aacttccgcg atctgacggg cgacaagccg
     1201 gcgggcattc ccaagtggag cctctcgacc tcggccaccg ccaaccacaa cttcggcaac
     1261 ggctattcgg gctatctgcg ggtcgagtat gactacacaa gtgacgttca gctgagtgag
     1321 accactccgc ccgagctgtc gacctggggt cagaaaaacg tcaatgccag cctgggcctg
     1381 ctgaatgaga cgcatcagct ggaagccatg ctttgggttc gcaacctgac caagcacgag
     1441 agcctgatca gcaccttccc gacggtggct caggacggca gctacagcgg ctatccgagt
     1501 gcgccccgca cgtacggcgt caccttgcgc aagacgttct agttcctcga cttagcgccg
     1561 ctcggccctc gtcgagcggc gctcttctct attccgaaat gacggcaatg ttctaagatg
     1621 tcgtcggtat cggctccagg atcatggtac taagttgccg aacgttagag cgcaaaattc
     1681 ctgacaatgg ggagttagcg agcagcggcc aagacggggt ctggcgctat aaggaccaat
     1741 cagggtgaag attggttttg agtgatggct tacgcatggt ggtagacatt gctggcaaca
     1801 gcgcgccagg attggcgcat gtctaggccg cttctatttc ttgatcccga tagcgatctc
     1861 agtcttcagg agcaggtgcg gcgcaaactg gtcgaggccg cgtacgccgg agcttttccg
     1921 cctggcaagc gcctgccgtc ttcgcgggga ctggcgaccc agttgggcgt ggcgcgcatt
     1981 accgtgacgc tggcctatca gaaattgctc gatgaggggt tttttgtcag tcgcgaacgc
     2041 agcggcattt atgtcgacca cgacatgctc cgcggcctta cagcggtcca gtcggtcgcg
     2101 ggccgccgcg aaccggcgcc atcctcaccc tggggcgcgc ggttcaagtc aagcgaggga
     2161 gatgaaccgg tcgcccgacg ctcgcccaac tggcgccagt atccgtatcc cttcattgac
     2221 ggtctttttg acgcctcgct gtttccggtt tcagagtggc gcgaggccag tcggcaggcc
     2281 ctggccctcg ctgacatcag ccagtggtca atggatggtg gcgatgcgga cgatccgatg
     2341 cttatcgagg agattcgcac caaggttctg ccacgacggg gaattcaggc ccggccggac
     2401 gagatactga ttacggtcgg cgcccagcag gcccttgccc tgatcagcca gatgctcgtc
     2461 gacaggacaa gcgtcgtcgc cgttgaaaac cccggctacc ccgacctgcg ccgactgttc
     2521 atggatcgcg gggcgcggct ggttcaccag ccggtcgatg acgagggaat ggtcgttgat
     2581 cggcggcttg acgactgcga tctggtctat gtcacgccaa gtcatcagtt tcccaccggt
     2641 gtgatgatga cccaggcgcg tcgggaagcc ttgctaaaga aggcttcagc gcgcgacctg
     2701 atcatcgtcg aggacgattt cgcctgcgag accaattatc tggacgaggc ctgtccggcc
     2761 cttcggagcc tggatcgcga tgaccgggtg atctatgtgg ccggcttttc aaaggtgctc
     2821 ggcccggggc tgcgattggg gttcatggtc gcctccgccg acgtgatcgc ggaagcgcgc
     2881 cggcttcgcc atctggcggt caggcacccg cccctgaaca accagcgcac ggcggcccat
     2941 tttctcgcca tgggccacta tgatgcgacg atgatgcgtc tgggccggct gtttcgggag
     3001 cgccgaacgg cgctgcgcga cgccctcaat cactatctcc agaacgccgt ggccatcgcg
     3061 cccttgcggg gcggcgccac ctactgggtg cggggcccag accatctaga cgtcgaggtg
     3121 tttgcggcgg aggcggaacg acggggcgtg ctgatcgagc cggtcggccc ctattttgcc
     3181 gacagcaagg cgccccggaa tatcttccgg ctcggggtca ccagtctgcc cctggaccgc
     3241 atccgtcagg gcgtggcggc cctggccgac ctgatgcggg acctgccggg gacggcccat
     3301 gcctttccgg atacggccag cgcccatttg gtgggcgcgg cgcttcagac cgcaatgtcc
     3361 ggcgccgtgc tactgtgcaa gacggtctat ggcgacccgt gcacgatcga gttgctgccc
     3421 aatggccgga tgtcgggccg ggccgggtac gccaacgaag actgcgacga gggccgatgg
     3481 tgggtcgagg gcgacttctg gtgccggcag tggagccgct ggagctatgg tgagacgtcc
     3541 aggctgatga cgacgattac tggagaccgc atcggctggt tcgacgctgg cgggcgcctg
     3601 gtggattcgg ccgtcattcg gcgtgcggat ctgtcatgac ctcttagggc cagatcattc
     3661 tttaggtgca ggccggctca gatctggccc tatacgcgcc cgacgactgg cgctatgggg
     3721 cagccgtttg cgcagtcact ctgagtgctc agggccgccc ggcccgcctt agagagttcc
     3781 catgcgccct atcggtatag ccggccttca actgcagctt ggcaagggcg acaatggcga
     3841 ccgcatcgcg gccgagatcg cggcggtaaa ggcgcgcctg ccctgggtgg acatggtggt
     3901 gctgggcgaa ctgtccctgt tcggggcgtc catttccacc gcccagccgc tgcctggcga
     3961 cgccgaggcc aagatgtgcg ccgccgcacg ggcgtccggc gtttggctgg tgcccggttc
     4021 gctgttcgag gcggatggtg gacgggttta caatactgcc ccggtgattg atccgaccgg
     4081 tcaggttgtc gctcgctatc gcaagatcta ccccttcctg ccctatgagc agggggtgac
     4141 ccccggaacc gagttcgtgg ttttcgacgt gcccggggtc ggccggttcg gtctgtcgat
     4201 ctgctacgac atgtggttcc cggagacgac ccggacgctg gtctggatgg gggcggaggt
     4261 catcatccat ccgggcatga ccaacaccat cgaccgtgag gccgaactgg ccgtcgcccg
     4321 cgccagcgcg gtgaccaacc aggtctattt tgtcgatatc aacaccgccg gtcagttggg
     4381 cgtcggcctg tcgggggtat tcgggcccgg aggcgaggtc attcatcagg cggggtcggg
     4441 ccatgaggtc atcgctgtcg aactggatct ggaccaggtc acccgggtgc gagagcgcgg
     4501 ctggcacggg ctagggcaac ccctgaagag cctgcgggac aatccgatca cctttccgcc
     4561 ctatcgggac ggatttgcgg cttcgccggc cttttcagga ctgggcccgt tgcaaaagcc
     4621 gggatcggaa cagccgggga agccccgttt ggggtgatca tggcgccggc ctgatccnnn
     4681 nnnnnnnaga tcggctgata aaaacggggg agtgagatga acaagtcgaa tctgagaggc
     4741 gctgcgtcga tcggtgcgct ggtcatggcc ctggcgtccg ccggcgccgg cgccgcgcag
     4801 gagatcaggg acaccacgat cggtgaaatc gtggtcaccg caaaccgtcg cgagcagagc
     4861 gttctggagg tgccctacaa catctcggcc gtctccggcg acgcgatcga ggacggcaag
     4921 atgctcgacg cagccgagct gatgcgcggc attcccggcg tctcggtagt cgatcgcggc
     4981 tatcgcaacg gcggcgtggt caacaacgtg ctgatccgcg gaatcaatgt cgatagcaac
     5041 gccctgggcg actatgcggt ctccagcgtc gctccggtgt cgacctatca gaatgatacg
     5101 ccactgttcg cagggttcct tctgaaggac ctcgaccgcg tggaagtgct gcgcgggccg
     5161 caaggcacgc tttacgggtc tggttccctg ggcggaactg tacgctacat cacgcgcgcg
     5221 ccccggcttg gtcagtttgg cggcgccgcc aacatcaccg ccagccaggt cgagggctcg
     5281 ggcagcaccg gctgggccgg cgacctgacc gtcaacctgc cgcttggcga catgttcgcc
     5341 ctgcgcgcca cggtcagcca gatcgattat ccgggcctca ccgactatgt gaacctgtat
     5401 gtgctggaca gcaccggcgc gccggcggcg cccagcggcg tcctgtcgcc caacgcggcc
     5461 tataccggca agaaggacgc tgacaccgtc gagatcagct ttgcgcgggt cgccctgttg
     5521 ttcgaaccga gtgaccggtt caacgccacc ctgacctaca gccagcagtc cgacgaggtc
     5581 ggtggccgac gccaggtgac gcgcggcctt gatggctatg gcaagcgcta cggcgactac
     5641 cagaacgggt ctattcagct agagccgtca tcgcgcgatg tcagcctc
//
LOCUS       DAOMEO010000036         5680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1870_length_5680_cov_2.709689, whole genome shotgun sequence.
ACCESSION   DAOMEO010000036 DAOMEO010000000
VERSION     DAOMEO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_5680_cov_2.709689"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLH31_01475"
     CDS             complement(<1..556)
                     /locus_tag="PLH31_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HQR87951.1"
                     /translation="MSGHSTYEPKTGFERWLDARLPIVRLGYDSFVDYPTPRNLNYWY
                     TFGGILSLCLATQLISGIILAMHYDVGDAFNSVERIMRDVNHGWLIRYMHANGASMFF
                     IAVYIHMFRGLYYGSYKAPREVLWLLGCVIYLLMMATAFMGYVLPWGQMSFHGAVVIT
                     NLFGALPIVGESITTWLWGGFAVDN"
     gene            complement(562..1113)
                     /gene="petA"
                     /locus_tag="PLH31_01480"
     CDS             complement(562..1113)
                     /gene="petA"
                     /locus_tag="PLH31_01480"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQR87952.1"
                     /translation="MADTAVGATTEPEHGNDPSRRDFIYIATIAAAAGGAASLVWPFI
                     DQMNPSADTLALASTEFDLTKVAEGQQVTLKWCGKPLFVRNRTAGEIQKAVADDSAAM
                     NDPQKDADRVKPGKAQWLVLIGSCTHLGCVPTFGGGDYGGWFCPCHGSHYDTSGRIRK
                     GPAPLNLAVPEYEFLSDTTVKIG"
     gene            1317..1781
                     /locus_tag="PLH31_01485"
     CDS             1317..1781
                     /locus_tag="PLH31_01485"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HQR87953.1"
                     /translation="MRLALFQPGIPQNLGAAIRLAACFGVGLDVIEPCSFPLDDKSLK
                     RAALDYGPLAHMKRHISWEAFLAAPERAEGRLLLFTTRGAVAFHRFSFAPGDTLLFGN
                     ESRGAPDAVHAAAQGRLIIPLRPEARSMNLATSAAMALGEALRQTDGFTDLA"
     gene            1895..2767
                     /gene="hemF"
                     /locus_tag="PLH31_01490"
     CDS             1895..2767
                     /gene="hemF"
                     /locus_tag="PLH31_01490"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HQR87954.1"
                     /translation="MTDALDLDQKKAAAKAWFETLRDQICAAFERLEDEAPADLYPGE
                     PGRFERKPWDREAGGGGVMSMMHGRLFEKVGVHCSTVFGTFTPEMAKAMPGADEDPRF
                     FATGISLIAHMVSPRVPAVHMNTRFIATTKSWFGGGGDLTPLLGYQRQQDFPDAVDFH
                     AAYQRACDAHDPTWHAKYKAWCDEYFFLPHRNEPRGIGGIFYDHHDSGDWSRDFVFTQ
                     DVGKAFLEIYPQLVRRRMGETWTAEEREQQLVQRGRYVEFNLLYDRGTMFGLKTGGNV
                     ESILSSMPPTVKWP"
     gene            complement(2780..3640)
                     /locus_tag="PLH31_01495"
     CDS             complement(2780..3640)
                     /locus_tag="PLH31_01495"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87955.1"
                     /translation="MTLYVSLRAGGGSRSMAAEACMLDGDGRLKRPSVRRLSPQEAAA
                     AVAGKTLVFVIHGFNVSYESGLRSLASLESELGKRLPENYRMIGVLWPGDFIIPAINY
                     PGEWRDAVESGRLLARFANRWMRAASDVCFLSHSLGGRVALEAVAHLDRKAAHVCLTA
                     AATDNDCLEAPYDVSVANADQMTWLASKKDVVLKLAYPVGDWAGDVFLGDDDSALRGA
                     LGRHGARWPRTGADTVRGVRLADGEKYGHGSYFPSGEFGAAPSPSSQAVARFAANFFK
                     GGGRPWPPPP"
     gene            complement(3645..4337)
                     /locus_tag="PLH31_01500"
     CDS             complement(3645..4337)
                     /locus_tag="PLH31_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87956.1"
                     /translation="MQNIDMVIAVIAATGGLGLAAMSLVDAFKAVPGGGVSRIGFRHI
                     RDVALLFDTVLERAVGAQWEPVILSHWINGRARSDQIGIVRSLLRLGLNPDTSDQLAA
                     IGNVDPKALSSAAGKLVKGAAMTEAEVNLIGRVEAAVEARLDAAFDLAEQAYRNQARI
                     LAGVIAVVLAMIASLLMEARPAISGLEWLDTRLLLGVLVGLLAVPIAPIAKDLVSALT
                     VAASAVKSTRRA"
     gene            complement(4404..5060)
                     /locus_tag="PLH31_01505"
     CDS             complement(4404..5060)
                     /locus_tag="PLH31_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HQR87957.1"
                     /translation="MRAATLIAVLGLALAGGAVITSVNAAPEGSAPGAATPAPVPLKA
                     AATFLSIKDPAARSAALFQEAGKVIESPRCMNCHPVERRPTQGDDLHPHNPPVAGGQS
                     GHGVPGLPCLSCHGPANVPTWGEKIQSIPGDPKWALAPVEMAWQGKSLGQICAQIKDP
                     ARNGGKTLDQIHHHMAEDSLVGWGWNPGAGRSPAPGTQAQFGQIIRAWIDTGAKCPKA
                     "
     gene            complement(5093..>5680)
                     /locus_tag="PLH31_01510"
     CDS             complement(5093..>5680)
                     /locus_tag="PLH31_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HQR87958.1"
                     /translation="YDVQAGQLPATPDEQDAYVITGSAAGVYDALPWIDPLSDFLRAA
                     RGQAALVGVCFGHQIMAQAFGGQVVKSDRGWGVGLHRYVVERPQGWTDGRSVAIPASH
                     QDQVVTLPPSATVVGGSPFTPYGLLAYDDVPAISMQFHPEFEPAYAAALIESRRGTRY
                     TDAQADAALATLQGPNDRARVGGWIRAFLREATGR"
BASE COUNT          962 a         1928 c         1870 g          920 t
ORIGIN      
        1 cattgtcgac ggcgaagccg ccccacagcc aggtggtgat gctctcgccc acgatcggca
       61 gggcgccgaa caggttggtg atcacgaccg cgccgtggaa ggacatctgg ccccagggca
      121 ggacgtagcc catgaacgcc gtggccatca tcagcaggta gatcacgcag cccagcagcc
      181 acagcacttc gcgcggagcc ttgtaggatc cgtaatagag gccgcggaac atgtggatat
      241 agacggcgat gaagaacatc gacgcgccgt tggcgtgcat gtagcggatc agccagccgt
      301 ggttgacgtc gcgcatgatg cgctcgacgc tgttgaaggc gtcgccgacg tcatagtgca
      361 tcgccaggat gatgccgctg atcaactggg tcgccagaca cagcgaaaga atgccgccga
      421 aggtgtacca gtagttcagg ttacgtggcg tcgggtagtc aacgaagctg tcgtagccca
      481 gacgcacgat cggaagacgg gcgtcgagcc agcgctcgaa accggtcttg ggttcatagg
      541 tcgaatgtcc gctcatctcg cttaaccgat cttgaccgtg gtgtcggaca ggaactcgta
      601 ctccggaacc gcgaggttca ggggcgccgg acccttacgg atccgccccg acgtgtcgta
      661 gtgcgagcca tggcaggggc agaaccagcc gccgtaatca ccgccgccaa aggtcggcac
      721 gcagcccaga tgggtgcagg aaccgatcag cacaagccac tgggccttgc ccggcttgac
      781 gcggtcagcg tctttctgcg ggtcgttcat cgccgcgctg tcatcggcga ccgccttctg
      841 gatttcgcca gcggtgcggt tgcggacaaa cagcggcttg ccgcaccatt tcagcgtcac
      901 ctgctgacct tcggcgacct tggtcaggtc gaactcggtc gaggccaggg ccagcgtgtc
      961 ggctgacggg ttcatctgat cgatgaacgg ccagacaagc gacgccgcgc cgccagcggc
     1021 agcggcgatc gtcgcgatat agatgaagtc gcgccggctg ggatcattcc catgttccgg
     1081 ctcggtcgtt gcgcccacgg cggtgtcggc caccttaagt cacccttcga ttccgccgcg
     1141 ccgagaaacc ggccggccag gagatgcgcc ttgcggcgcc gtatcgttct cgacccgcaa
     1201 gcggatctcc gccacaaggc gacggttgga tcgtcctatc ggacctgcgg cacgagtccg
     1261 caagcccttt cgattcgcag caatgtccct gctagccttg tggtggttcg cttagaatgc
     1321 gtctcgcgct ttttcaacca ggcattcctc aaaacctcgg cgccgcgatc cgcctcgccg
     1381 cctgttttgg cgtcggcctg gacgtgattg agccctgcag cttccccctg gacgacaaaa
     1441 gtctcaagcg ggcggcgctg gattatgggc cgctggccca catgaaacgc catatttcct
     1501 gggaagcctt cctcgccgcg cctgaacgcg ccgaaggccg gctgctgctg ttcacgacca
     1561 ggggcgccgt cgccttccac aggttttcct tcgctccggg cgatacactt ttgtttggca
     1621 acgaaagccg gggcgcgccc gacgcggttc acgccgcggc gcagggccga ctgatcatcc
     1681 cgctgcggcc cgaagcccgg tcgatgaatc tggccaccag cgccgccatg gcgctgggcg
     1741 aggcgctgag acagaccgac gggttcaccg acctggccta aaccctgggg catgcgacat
     1801 tttgcctggg agtgccgccc gctccggccc ggaggccagc cttacgcatc atcccggatt
     1861 tgcgcgggac gctggttggc cttaagcggc tcccatgact gacgccctcg atctcgacca
     1921 aaagaaagct gcggcgaaag cctggtttga aaccctgcgc gaccagatct gcgcggcgtt
     1981 cgagcgcctg gaggacgagg cccccgccga cctctatccg ggcgagcccg gccgcttcga
     2041 gcgcaagccg tgggaccggg aggccggcgg cggcggcgtc atgtcgatga tgcacggccg
     2101 cctgttcgag aaggtgggcg tccactgctc caccgtgttt gggaccttca cgcccgagat
     2161 ggccaaggcg atgccgggcg cggacgagga tccccgcttc ttcgccaccg gcatcagcct
     2221 gatcgcccac atggtcagcc cgcgcgtgcc ggccgtgcac atgaacaccc gctttatcgc
     2281 caccacgaaa agctggtttg gcggcggcgg cgacctgacc cccctgctgg gctatcagcg
     2341 ccagcaggac tttccggacg ccgtcgactt ccatgccgcc tatcagcgcg cctgcgacgc
     2401 ccacgacccg acctggcacg ccaagtacaa ggcctggtgc gacgagtatt tcttcctgcc
     2461 acatcgcaac gagccgcgcg gcatcggcgg catcttctat gaccaccacg acagcggcga
     2521 ctggtcgcgc gacttcgtct tcacgcagga cgtcggcaag gcgtttctgg agatctatcc
     2581 gcagctggtc cgtcgccgga tgggcgagac ctggaccgcc gaggagcgcg agcagcagct
     2641 ggtgcagcgc ggacgctatg tggagttcaa cctgctttac gaccgcggca cgatgtttgg
     2701 tctgaagacc ggcggcaatg tcgagtcgat cctcagctcg atgccgccca cggtgaagtg
     2761 gccctgagcc aggcaagtct cagggcggcg gcggccacgg tcgtccgcca cccttgaaga
     2821 agttggcggc gaatcttgcg acggcctgac tgctgggact gggcgccgcg ccgaattcac
     2881 cggacggaaa atagctgccg tggccgtact tttcgccgtc ggccagcctg acgccccgga
     2941 cagtgtcggc gcccgtgcgc ggccatctgg ccccatggcg gcccagggcg ccgcgcaggg
     3001 cgctgtcatc atcacccaga aagacatcgc ccgcccagtc gccgaccgga taggccagct
     3061 tcagcaccac gtcctttttc gaggccagcc aggtcatctg gtcggcgttg gcgacgctga
     3121 cgtcataggg cgcttccagg cagtcattgt cggtcgccgc tgcggtcagg cagacatggg
     3181 cggccttgcg gtccaggtgg gccaccgcct ccagggccac gcggccgccc aggctgtgcg
     3241 acaggaagca gacgtcgctg gccgcccgca tccaccggtt ggcgaagcgg gccagcaggc
     3301 ggccgctttc gaccgcgtcg cgccactccc ccggatagtt gatggccgga atgatgaaat
     3361 cgccgggcca cagcaccccg atcatccggt agttttcggg caggcgcttg cccagttcgc
     3421 tctccagtga ggccagggag cgcagcccgc tctcatagct gacattgaag ccgtggatga
     3481 cgaacaccaa ggtcttgcct gcgacggcgg ctgcggcctc ctgggggctc agccgtctga
     3541 cgcttggtcg cttgaggcgg ccatcgccgt ccagcatgca cgcctcggcg gccatgctgc
     3601 gactgccgcc gccagcccgc agggacacat acagcgtcat ggcgctaggc cctgcgcgtc
     3661 gacttgaccg cgctggcggc gaccgtcagg gcgctgacca gatccttggc gatcggcgcg
     3721 atcggcacgg ccagcaggcc caccagcacc ccgagcagca accgcgtgtc cagccattcc
     3781 aggcccgaaa tcgccgggcg cgcctccatc agtaacgagg cgatcatggc caggaccacc
     3841 gcgatcacgc ccgccaggat gcgcgcctga ttgcgatagg cctgttcagc cagatcgaag
     3901 gccgcgtcca ggcgcgcctc gaccgccgcc tcgacccggc caatcagatt gacctcggcc
     3961 tcggtcatcg ccgcgccctt gaccagcttg ccagcggcgc tgctcagggc cttggggtcg
     4021 acattgccga tggcggccag ctggtcggag gtgtcgggat tgaggcccag gcgcagcaag
     4081 gaccggacga tgccgatctg gtcgctccgt gctcgcccat tgatccagtg cgacaggatc
     4141 acgggctccc actgggcccc gaccgcgcgc tccagcaccg tgtcgaacag tagtgcgacg
     4201 tcccggatgt ggcgaaagcc gatccgcgag acgccgcccc cgggtacggc cttgaaggcg
     4261 tcgaccagac tcatggcggc cagtcccaga ccgccggtcg cggcgatgac ggcgatgacc
     4321 atgtcgatat tctgcatgcg cccctcccct aaaggaggaa cgatgttatc tcaaagcgcc
     4381 ccagaacaac agggggttgt acttcaggcc ttggggcact tggctccggt atcgatccag
     4441 gcgcggatga tctggccaaa ctgggcctgg gttccagggg ccggcgagcg gcctgcgccc
     4501 gggttccagc cccagcccac cagagagtcc tcggccatgt gatggtggat ctgatccagg
     4561 gtcttgccgc cgttgcgggc cgggtccttg atctgggcgc agatctgacc cagcgacttg
     4621 ccctgccagg ccatctcgac cggcgcgagg gcccatttcg gatcgccggg gatgctctgg
     4681 atcttctcgc cccaggtcgg aacattggcg gggccatggc acgacaggca cggcaggccg
     4741 ggcacgccgt gaccgctctg gcccccggcg acgggcggat tgtgcgggtg caggtcgtcg
     4801 ccctgggtgg gccggcgctc gaccgggtgg cagttcatgc agcgagggct ctcgatgacc
     4861 ttgcccgcct cctggaacag cgctgccgac cgggcggccg gatccttgat cgagaggaag
     4921 gtcgcagccg ccttcagcgg gaccggagcg ggcgtggccg cccctggcgc gctcccctcg
     4981 ggcgccgcat tgacgctggt gatgacagcg ccgcccgcga gggcgaggcc taaaacggcg
     5041 ataagggtgg cggcgcgcat gtcagggtct ccctccgaca gcaacgctct agctagcggc
     5101 cggttgcctc acgcaggaaa gcccggatcc agccgcccac ccgcgcgcgg tcattgggcc
     5161 cctgcagcgt ggcgagggcg gcgtcggcct gggcgtcggt gtagcgcgtc ccgcgtcgcg
     5221 actcgatcag ggccgccgca taggctggct cgaactcggg gtggaactgc atcgaaatgg
     5281 ccggaacgtc gtcataggcc aacaggccat agggcgtgaa ggggctgcca ccgaccacgg
     5341 tcgccgaggg cggcagggtc accacctgat cctggtgcga cgcgggaatg gcgacgctgc
     5401 gcccatcggt ccagccctgg gggcgctcga cgacgtagcg atgcagaccc acgccccagc
     5461 cacgatcaga cttgaccacc tggcccccga acgcctgggc catgatctgg tggccaaaac
     5521 agaccccgac cagagcggcc tgcccccgcg ccgctctgag aaaatccgac aacggatcaa
     5581 tccagggcaa ggcgtcatag accccggccg ccgagccggt gatgacatag gcgtcctgct
     5641 cgtccggcgt ggccggaagc tggccagcct ggacatcata
//
LOCUS       DAOMEO010000037         5655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1875_length_5655_cov_2.870893, whole genome shotgun sequence.
ACCESSION   DAOMEO010000037 DAOMEO010000000
VERSION     DAOMEO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_5655_cov_2.870893"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1484
                     /locus_tag="PLH31_01515"
     CDS             186..1484
                     /locus_tag="PLH31_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87959.1"
                     /translation="MITRLASLAAALLLGAAAPALAASAGGPKLDVIARDFVALTLEA
                     GEREPGYVDAYYGPKEWAAAAKANPRAVPALKAEAIRLTAATKAVDDASLTPDERRRK
                     AFMLGQLKAAETRLAMLEGQKFSFQDEAEGLFSVRPPIKPLESFDPILARIDKLVPGD
                     GDLAARVDAFQNKYVIPADKLDAVMKAGIAACKARTQAHIKLPKDEKFDLAFVTGKPW
                     SGYNWYKGGAHSLIEINTDLPVAISRAVDLGCHEGYPGHHVLNAMLEDKLTNAKGWVE
                     FSVYPLYSPQSLIAEGSANYGIALAFPGAEKVKFEREQLYPLAGLDPKTADTYDALMA
                     AKADLASSENTIADAYLSGKMDKPAAIAALAKYGLSSVARAEKRLSFIETYRSYVINY
                     NLGQEMVRAHVERAGPSQDARWKAMEAVISEPTTPADLAR"
     gene            complement(1644..3497)
                     /locus_tag="PLH31_01520"
     CDS             complement(1644..3497)
                     /locus_tag="PLH31_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQR87960.1"
                     /translation="MMTDVNGGEISVEGRPGAGAELAQGMAEAAARRPRRKDIRPLAH
                     LLPFVRAHVGDALATGFFLLFSTGATLGLTAAFKGVIDKGFASGAEADVNGAFLGLGA
                     VALVLAVASAARFFFVTRLGERVVADLRRALYGHTLTLDPAYFLKTRTGEVLSRLTTD
                     VTIVEQLVGTSASVAVRNLLSLVGGLGYMAVVSPKLAGFIVLMVPVVLAPMFLMGRKV
                     RKLSVTAQDRFADAVGYAGESLDALDTVQAFGRAKASFGRFGEAVEMAYQASVRRISA
                     RAGMTAVVIVLAFGGITLLLWTGARLVLAGEMTPGTLAQFALLAIMAAGSIGALGEVW
                     GDVQKAAGAMERIAELLNSRPGIAAPEKPRTLPTPATGAVAFENVTFAYPGRPDLPAL
                     RGFDLTVRPGETVALVGPSGAGKSTVLRLLLRFYDPQEGVIRLDGVDLRDADPAEVRQ
                     RMALVAQDSPLFSGSALDNIRFGREDATEEEARTAAAAAQATGFLEALPQGFDTPVGE
                     RAKTLSGGQRQRLAIARALVRQAPILLLDEATSALDAENERLVQQALHAAMAGRTTLV
                     IAHRLATVLKADRIVVMEDGRVVEQGTHAELSARGGLYSRLAKLQFGVEAA"
     gene            3609..3833
                     /gene="rpmE"
                     /locus_tag="PLH31_01525"
     CDS             3609..3833
                     /gene="rpmE"
                     /locus_tag="PLH31_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQR87961.1"
                     /translation="MKQDIHPDYHFITVTMTDGTSYPTRSTYGKEGSVLALDIDPRVH
                     PAWTGGNAHLLDRGGRVSRFNAKFAGFTKK"
     gene            complement(3970..4482)
                     /locus_tag="PLH31_01530"
     CDS             complement(3970..4482)
                     /locus_tag="PLH31_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HQR87962.1"
                     /translation="MTELNASASTPWRAGVIQDFAKSELFDRTFEEGMMLVEETAGYL
                     DGAGRHDSKILSRNAALAYATESMRLTTRLMQVASWLLVQRAVKEGEMAAEAACAESY
                     RLAEEAVVIGAAVEELPYGLVNLLQRSERLYERVRHLDKRMYVEVALEDAPRPVQAQF
                     DRLAAAFGAA"
     gene            complement(4655..4861)
                     /locus_tag="PLH31_01535"
     CDS             complement(4655..4861)
                     /locus_tag="PLH31_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 family protein"
                     /protein_id="HQR87963.1"
                     /translation="MFEEPVEPRAFSGRALSETTHEDLEIYGVAELEERIEALQAEIT
                     RTRTQLDKKRAGRDAANALFGRLD"
     gene            4953..>5655
                     /locus_tag="PLH31_01540"
     CDS             4953..>5655
                     /locus_tag="PLH31_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR87964.1"
                     /translation="MSDTMTAIAIDGGKGPADALVPTTLERPVPGPGEILIKVAAAGV
                     NRPDLIQRLGYYPPPPGAPATLGLEVAGEVVALGEGGGRWRVGDKVCALLGGGGYAQY
                     AVTDARHALPIPAGFDMVRAAALPETVFTVYANVFEHGALKAGETLLIHGGTSGIGTT
                     AIAMAKAAGARVIATGRGADKKAKALELGADIAVDTRTEDFAEIARAAGGVDVVLDMV
                     GASYFEKNLQALNIGG"
BASE COUNT          948 a         1998 c         1874 g          835 t
ORIGIN      
        1 atggccctgc ccggcgaggt ggcgccgctg ctgaaggccc tctacgcggc cggcgtcgat
       61 ggggtgttca gcgacttccc ggacctggcg gtcgccgcgc gctgagtttc attacatttg
      121 gttcatttgc cgcccgcccc gcgcgggggc agcgtgcgcg cctcacgttc gatcgaggcg
      181 cttcaatgat cacccgtctg gccagcctgg ccgccgctct cctgctcggg gccgccgcgc
      241 ccgccctcgc cgcttccgct ggcggtccca agctggacgt catcgccagg gacttcgtgg
      301 ccctgacgct ggaggccggc gagcgcgagc ccggctatgt cgacgcctat tacggcccca
      361 aggaatgggc cgccgccgcc aaggccaatc cgcgcgccgt gccagccctg aaggccgagg
      421 ccatccgcct gaccgccgcg accaaggccg tcgatgacgc ctcgctgacg cccgacgagc
      481 gccgccgcaa ggcgttcatg ctgggccagc tgaaggccgc cgaaacccgc ctcgccatgc
      541 tggaggggca aaagttcagc ttccaggacg aggccgaggg cttgttcagc gtccgcccgc
      601 cgatcaagcc cctggaaagt ttcgacccga tcctggcccg catcgacaag ctggtccccg
      661 gcgacggcga cctggccgcc cgcgtcgacg ccttccagaa caagtacgtc atcccggctg
      721 acaagctgga cgcggtgatg aaggccggca tcgcggcctg caaggccagg acgcaggctc
      781 acatcaagct gcccaaggat gagaagttcg acctggcctt cgtcaccggc aagccctgga
      841 gcggctacaa ctggtacaag ggcggggccc acagcctgat cgagatcaat accgacctgc
      901 cggtcgccat cagccgcgcc gtcgacctgg gctgccacga gggctatccg ggccaccacg
      961 tcctgaacgc catgctggaa gacaagctga ccaacgccaa gggctgggtc gagttcagcg
     1021 tctatcccct ctattcgccg caatccctga tcgccgaggg ctcggccaat tacggcatcg
     1081 ccctggcctt cccgggcgcg gagaaggtca agttcgagcg cgaacagctc tatccgctgg
     1141 cgggcctcga ccccaagacc gccgacacct acgacgccct gatggccgcc aaggccgatc
     1201 tggccagcag cgaaaacacc atcgccgacg cctatctgtc gggaaagatg gacaaacccg
     1261 ccgccatcgc ggccttggcc aaatacggcc tcagctcggt ggcccgcgcc gagaagcgcc
     1321 tgagcttcat cgagacctac cggtcctacg tgatcaacta caacctcggc caagagatgg
     1381 tccgggcgca tgtcgagcgg gcggggccga gccaggacgc gcgctggaag gcgatggaag
     1441 cggtgatcag cgagcctacg acaccggctg atttggcgag gtagtcagcg aagcccttct
     1501 ccccttgcgg gagaaggtgg cgaccgcacg tcgacggatg aggggtcgcg caatcgccga
     1561 cgcctcatcg cagcggaact gaccttcgac ccctcatccg gccgctatcg cggccacctt
     1621 ctcccgcaag gggagaaggc agatcaagcc gcctccaccc cgaactgcag tttcgccagc
     1681 cgcgaataca gcccgccgcg cgccgaaagc tcggcgtggg tgccctgctc cacgacgcgg
     1741 ccgtcctcca tgaccacgat gcggtcggct ttcaggaccg tggccaggcg gtgggcgatg
     1801 accagggtgg tgcggccggc catcgccgcg tgcagggcct gctggaccag gcgctcgttc
     1861 tcggcgtcca gggcgctggt ggcctcgtcg agcagcagga tcggggcctg gcgcaccagg
     1921 gcgcgggcga tggccaggcg ctggcgctgg ccgcccgaca gggtcttggc gcgctctccc
     1981 accggggtgt cgaagccctg cggaagggcc tcgaggaagc cggtggcctg ggcggcggcg
     2041 gcggcggtgc gggcttcttc ctcggtggcg tcctcgcggc caaagcggat gttgtccagg
     2101 gccgagcccg agaacagcgg cgagtcctgg gccaccaggg ccatgcgctg gcgcacctcc
     2161 gcaggatcag cgtcgcgcag atcgacgccg tccagacgga tgacgccttc ctgcgggtcg
     2221 tagaagcgca gtagcagccg caggacggtg ctcttgcccg cgccagaggg tcccaccagg
     2281 gcgacggtct cgccgggcct gacggtcaaa tcaaagcccc gcagcgccgg cagatccggg
     2341 cggccgggat aggcgaaggt gacgttctcg aaggcgaccg cgcccgtggc gggggtcggc
     2401 agggtcctgg gcttctccgg cgcggcgatg ccgggccgcg agttcagcag ctcggcgatg
     2461 cgctccatgg cgccggcggc cttctggaca tcgccccaga cctcgcccag ggcgccgatc
     2521 gagccggcgg ccatgatggc cagcagggcg aactgggcca gggtgccggg ggtcatctcg
     2581 ccggccagca ccagacgtgc tccggtccac agcagcaggg tgatgccgcc aaacgccagc
     2641 acgatgacca ccgccgtcat gcccgcccgg gcgctgatgc ggcgcacaga ggcctggtag
     2701 gccatctcta cggcttcacc aaagcggcca aacgaggcct tcgcccggcc aaaggcctgg
     2761 acggtgtcga gcgcgtccag gctctcgccg gcatagccca ccgcgtcggc gaaacgatcc
     2821 tgggccgtca ccgacagctt gcgcaccttg cgccccatca ggaacatcgg ggccagcacc
     2881 accggcacca tcagcacgat gaagccggcc agcttggggc tgaccaccgc catatagccc
     2941 aggccgccca cgaggctgag aaggttgcgc accgccaccg acgccgaggt gcccaccagt
     3001 tgctcgacga tcgtgacgtc ggtggtcagg cgcgacaaaa cctcgccggt gcgggttttc
     3061 aggaaatagg ccggatccag cgtcagggta tggccataga gcgcgcggcg caggtcggcc
     3121 acgacccgct cgcccagccg ggtgacgaag aagaaccgcg cggcgctggc cacggccagc
     3181 accagggcca cggcgccaag gcccaggaaa gcgccattga cgtccgcctc ggccccggac
     3241 gcaaacccct tgtcgataac gcccttgaag gccgccgtca ggcccagggt ggcgccggtg
     3301 gagaacagca ggaagaagcc ggtggccagg gcgtcgccca catgggcgcg cacgaacggc
     3361 agcagatggg ccaggggacg gatgtccttg cggcgggggc ggcgggcggc ggcctcggcc
     3421 atgccctggg ccagttcggc cccggcgccg gggcgtccct cgaccgaaat ctctccgccg
     3481 ttcacatcgg tcatcaactg tcccttgcca aattcgcgcg tgttctttat agccgcgcct
     3541 cttcgagggc cacccgcaag ggagtccgct caccaacttt cacaccgcca gtcggttgga
     3601 ctcgcccgat gaaacaagac attcaccccg actatcactt catcaccgtc accatgaccg
     3661 atggcaccag ctatccgacc cgttcgacgt acgggaaaga aggctcggtc ctggcgctgg
     3721 atatcgatcc gcgcgtccac ccggcctgga ccggcggcaa cgcccacctg ctagaccgtg
     3781 gcggccgcgt ctcgcgcttc aacgccaagt tcgcgggctt caccaagaag tagcccttct
     3841 cgccgcaagg gcgacgaaaa gccggctccg cgtttgcggg ccggcttttt ctgtgcctgg
     3901 tgctcaggcg tcaaaaaagc ccgccctctc ggcgagaggg cgggcttttt gttgggatga
     3961 tggggtggct taagccgcgc cgaacgctgc cgcgaggcgg tcgaactggg cctggaccgg
     4021 gcggggcgcg tcctcgaggg cgacctcgac atacatccgc ttgtccaggt gacgcacgcg
     4081 ctcgtacagg cgctcggagc gctgcagcag gttgaccagg ccgtagggca gttcctcgac
     4141 cgcggcgccg atcacgacgg cttcctcggc caggcggtag ctttcggcgc aggcggcctc
     4201 ggcggccatc tcgccttcct tcaccgcgcg ctgcaccagc agccacgagg ccacctgcat
     4261 caggcgggtg gtcaggcgca tgctctcggt ggcataggcc aaggcggcgt tgcgcgagag
     4321 gatcttgctg tcgtgccgac cggcgccgtc gagatagccg gcggtctcct cgaccagcat
     4381 catgccttcc tcgaacgtgc ggtcgaacag ctccgacttg gcgaagtcct ggatgactcc
     4441 ggcgcgccag ggcgtcgacg cggatgcgtt cagttcggtc ataggtactc aatgcccctt
     4501 cgaaaggccg cgagaacgtc tcgcctgacg caaggactgc aacccccgtg ccaaccgaac
     4561 ccgtcccgtt tcgcgacccg cccccttcga acctcgacac gtctggaaca aaggcggtta
     4621 ccgcctttag atattgatta aggtaaacga atccctagtc gaggcgacca aacagcgcat
     4681 tggcggcgtc gcgccccgcg cgcttcttgt ccagctgggt tctggtgcgc gtgatttcgg
     4741 cctgcagggc ctcgatccgc tcctccagct cggccacccc atagatctca aggtcttcgt
     4801 gtgtcgtctc actgagggcg cgaccactga aagcgcgcgg ctcgacgggt tcctcgaaca
     4861 tcgactggct ccccaagacc tatgaacgtc cttttcgatc ctccatctga cgcctaccta
     4921 gggcgcaagg caagcttgag ggatcacaga ccatgtccga cacgatgacc gcgatcgcca
     4981 tcgacggcgg caagggaccg gccgacgctc tggtccccac caccctggag cgcccggttc
     5041 ccggccccgg cgagatcctg atcaaggtgg cggcggccgg ggtcaatcgc cccgacctga
     5101 tccagcgcct gggctattac ccgcccccgc ccggcgcgcc ggccaccctg ggcctggagg
     5161 tcgccggcga ggtcgtcgcc ctgggcgagg gcgggggtcg ctggcgcgtc ggcgacaagg
     5221 tctgcgccct gctgggcggc ggcggctatg cgcaatatgc cgtgaccgac gcccgccacg
     5281 ccctgccgat cccggccggc tttgacatgg tccgcgccgc cgccctgccc gagacggttt
     5341 tcaccgtgta cgcgaatgtg ttcgagcatg gcgcgctgaa ggccggcgag accctgctga
     5401 tccacggcgg aacgtccgga atcggaacaa ccgccatcgc catggccaag gcggccggcg
     5461 cccgggtgat cgccaccggt cgcggcgccg acaagaaggc caaggccctg gagctgggcg
     5521 ccgacatcgc cgtcgacacc aggaccgagg acttcgctga aatcgcccgc gcggccggcg
     5581 gcgtcgacgt ggtgctcgac atggtcgggg ccagctattt cgagaaaaac ctgcaggcgc
     5641 tgaacatcgg cggcc
//
LOCUS       DAOMEO010000038         5596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1886_length_5596_cov_2.467605, whole genome shotgun sequence.
ACCESSION   DAOMEO010000038 DAOMEO010000000
VERSION     DAOMEO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_5596_cov_2.467605"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLH31_01545"
     CDS             complement(<1..639)
                     /locus_tag="PLH31_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87965.1"
                     /translation="MPTVVEQIQLEAVSSETPVSQLLRRVKLAAVKLKLKTVAAWVDH
                     EIDGYGDAPVPDYRIVAGAPVAHNPYNGWIPIQTSVEQMDSLRRLKIRQSIASLEALI
                     PGDGTIYFQYSPAMIDWINSGVDIPFARMGVQLDRSAIIGLLDRVRGKVLEWALELEL
                     AGITGEGVSFTTQEVERALSMALQIENFTGTLNTGVANGPNARINNNSTDNST"
     gene            complement(689..>1106)
                     /locus_tag="PLH31_01550"
     CDS             complement(689..>1106)
                     /locus_tag="PLH31_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQR87966.1"
                     /translation="AVTRLGFKPRVAFMSYSTFGNPMGLRSEKVREAVAMLDEMDVDF
                     EYEGEMPPELALDPEKRANYPFMRLTDSANILIMPAIHAASISTKLVQSLGGATVIGP
                     VLLGLSKSVQITPLSASVSRILNMAMMAAYDAPAEG"
     assembly_gap    1107..1116
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1117..2960)
                     /locus_tag="PLH31_01555"
                     /pseudo
     CDS             complement(1117..2960)
                     /locus_tag="PLH31_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288959.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
     gene            3185..3958
                     /gene="dapB"
                     /locus_tag="PLH31_01560"
     CDS             3185..3958
                     /gene="dapB"
                     /locus_tag="PLH31_01560"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519037.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQR87967.1"
                     /translation="MTTPVKIAVAGALGRMGQAVARTLDGHALATLAARFDRPGAEGP
                     GLVERDAALEGADAVIDFTLPEASVLLAETCAQRGGPAVVIGSTGFSAEQIARIDAAA
                     TKIAIVRSGNYSLGVNMLMGLVKRAAAALPASEWDIEVFEAHHKRKIDAPSGTALMLG
                     QAAADGRGVALSDVEERARDGVTGARTQGAIGFSVVRGGGIIGEHSVIFAGEEEVLTL
                     SHSATDRGLFARGAVAAAVWAVGRPPGLYDMQDVLGFRE"
     gene            4017..>5596
                     /locus_tag="PLH31_01565"
     CDS             4017..>5596
                     /locus_tag="PLH31_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87968.1"
                     /translation="MAEPTPDPAPPTRAFRRRERLIDAGLEAVSLSMLAFIAVGLLAH
                     GGRREISRELAQSWLRERGIDAVVVVDDLDASGFSGSVLLGSKSDPIFAADRIEVAYD
                     LTTPWNGDGFAVDTRAVRLVRPRIKASLTEQGGLNFGALQPLIDEALASPKDPKATGP
                     AILVENARLDLRTPGGLARVTGDASLDNGKLLRFDGRLARMRYATGDLVIEARGALLS
                     ARKRGERLTVAASFDIDALVQPALELDDAQGQIDADLAYPDLVKLSATGPAEVRLALK
                     AASARAGATRINDAATTLALLGVLDGTLTQGRFEGQVTGKARGETLRTQGLDARDISL
                     DLNLTQVAASRLGEKIAVQTRGHVQAEAGHALASGAALREVSARLDLAGLILSADKAG
                     AVVSGPLALQLGASRLASGGLALNSLAVEARGRVAGPLTHPILTASGSAGADSGVAGP
                     DAERLAAALPDPADARAVAQALQTFDLRAPAFDLTLRDGRTILALPRPITLTAANGVR
                     ASLAAPRGPLLDALAGQMR"
BASE COUNT          871 a         1851 c         1907 g          957 t
ORIGIN      
        1 cgtcgaatta tcggtggaat tgttattgat ccgggcattt gggccgttgg cgacgccagt
       61 gttgagcgtt ccagtgaaat tttcgatctg caaagccatg ctcaacgctc tttcaacttc
      121 ctgggtagtg aagctgactc cctcccccgt tattcccgct aactcaagct ccaatgccca
      181 ttccaagact tttccacgga cccgatcaag cagaccaatt atggcactac ggtcaagttg
      241 cacacccatg cgggcaaacg gaatatccac gccgctattg atccaatcga tcatcgcggg
      301 cgaatattga aaatatattg ttccatcgcc aggaattaaa gcctctaggc tcgctataga
      361 ctggcgaatt ttcaatcgcc ttagactatc catctgctca actgaggtct ggattgggat
      421 ccagccatta tatggattgt gtgcaaccgg cgcacccgcg acgatccggt agtccgggac
      481 aggcgcatca ccgtagccat cgatctcatg gtccacccaa gccgcgacgg tcttgagctt
      541 gagcttaact gccgccaact ttacgcggcg aagcagctga gagacggggg tctcgctcga
      601 cacggcttcg agctgaattt gttcgaccac cgttggcatg ctcactcctg ttctcaggcg
      661 ctcaatggac gcgatccgga ctacctcgtc acccctccgc cggcgcgtca tacgccgcca
      721 tcatcgccat attcagaatc cgcgacaccg acgccgacaa cggcgtgatc tgcaccgact
      781 tgctcaggcc cagcagcacc ggcccgatca ccgtcgcccc gcccaggctc tgcaccagct
      841 tggtcgagat cgaggcggcg tggatggccg gcatgatcag gatgttggcg ctgtcggtca
      901 ggcgcatgaa cgggtagttg gcgcgctttt cggggtcgag cgccagttcg ggcggcattt
      961 cgccctcata ttcgaagtcg acgtccatct cgtcgagcat ggcgacggcc tcgcgcacct
     1021 tttccgagcg caggcccatc gggttgccga aggtggaata gctcatgaag gcgacgcgcg
     1081 gcttgaagcc caggcgggtg acggcgnnnn nnnnnntcgc aggcgatctc gaccagctcc
     1141 tcggcctcgg gcagttcggt gacattggtg tcagccacga agatggtgcg gcccttggcc
     1201 agcaccaccg acatgcccat gatccggccg ccgggggcgg gatcgacgac gcgcagcacg
     1261 tcttccaggg cctggtcgaa gctgcgggtg acgccggtga ccatgccgtc ggcctcgcca
     1321 tgggtgacca tcgaggcggc gaaactgttg cggtcctggt tgatcaggcg ctggacgtcg
     1381 cgcttcagat agccgtggcg ttgcagacgg gcatagaggg cgtcgacata gtcaggattg
     1441 cggtcgctga ggcgggcgtt gatgatttcc aggcccgccg tctcggggtc caggcccacg
     1501 acgcgcatgt tctcggccac caggttctcg cggccgcaca ggatggcctt gccgtagccg
     1561 ccggtctgat aggcgtaggc ggcgcggatc accgagggat cctcgccgaa gacgatgcgc
     1621 ttgggattgg ccagcacggc gccggcgatc ttctgcagga agccggcggt gggatccagg
     1681 cgctgggcca ggctggcgcg ataggcgtcc atgtcctcga tgggcttcct ggccacgccg
     1741 gtgtccatcg ccgcctgggc gacgaagggc ggcacgtacc agatcaggcg cgggtcgaag
     1801 gccgagggga tgatgtattg cgggccaaac ttcagctggc ggccgtgata ggcggcggcc
     1861 acttcatccg gaacgtcctc gcgggccagc atggccaggg cgttggcgca ggcgatcttc
     1921 atctcgtgat tgacgcggcg ggcccgcacg tccagcgcgc cgcggaagat gtagggaaag
     1981 cccaggacgt tattgacctg gttgggatag tcgctgcggc cggtgcccat gatggcgtcc
     2041 ttgcggacgg cgtggacctc ctcgggcgta atctcgggat cgggattggc catggcgaag
     2101 atcaccggat tgggcgccat gctggcaatc atttccgggg tgaaggcccc cttggccgag
     2161 agccccagca gcacgtcggc gcccttgacc gcttcagcca gggtgcgctt gtcggtctcg
     2221 acggcgtggg cgctcttcca ctggttcatg ccctcggtgc ggccgcgata gagcacgccc
     2281 ttggagtcga cggcgatgca gttctcggcg cgcacgccca tggccttgat cagctccagc
     2341 gaggcgatgc ccgccgcgcc ggggccgttg agcaccacct tgacgtcttc cagcttcttg
     2401 ccggtgatgt ggcaggcgtt gatcaggccg gcggcgcaga tgatcgccgt gccgtgctgg
     2461 tcatcgtgga acaccgggat gtcgagcagt tcctgcagct cggtctcgat gcggaagcat
     2521 tcggggctct tgatgtcttc cagattgatg ccaccgaagg tgacgccgat attcttgacc
     2581 accgtgatga tctcatcggc gtccttggag ctgatctcga tgtcgatgct gtcgacgtcg
     2641 ccaaagcgct tgaagaggac gctcttgcct tccatcaccg gcttggaggc cagcgcgccg
     2701 agatcaccta aacccagaat ggcggtgccg ttggagatca ccgccaccag gttgcccttg
     2761 gaggtgtatt cgtaggccat gtccgggtcg gcggcgatgg cgtggacggg tacggcgacg
     2821 ccgggcgaat aggccagcga caggtcgcgc tgggtggcca tcggcttggt tgggacgatc
     2881 gcgatcttgc cgggggtcgg atagcggtgg aaggccagcg cttcttcgtc ggtgaaggtc
     2941 ttcttctcgg tgtcgctcat aacccgcgtt tccgtcctgc atgccagttg aggggacgag
     3001 ccggtcaatg cggagcgccc ttatgggcgg caccgtagcg agggcgcggc gccctcacaa
     3061 cctgcggcgc ggcataaaaa agcgcgcaaa cgcttgttgg gcttggcgtt gcgtcaacct
     3121 ggggcaggcc cgcgcagacg atccgcgatt gaccgaaggc ccaggaatgg gtgactaggc
     3181 ggtcatgaca acgcctgtga aaatcgccgt cgccggagcc ctgggccgca tgggtcaggc
     3241 cgtggccagg acgctggacg gtcatgccct ggcgaccctg gccgcgcggt tcgaccgtcc
     3301 gggcgcggaa ggtcctggcc tggtcgagcg cgacgcggcc ctcgagggcg ccgacgcggt
     3361 gatcgacttc accctgcccg aggcctcggt gctgctggcc gagacttgcg cgcagcgcgg
     3421 cgggcccgcc gtggtgatcg gctcgaccgg cttttcggcc gaacagatcg cccgtatcga
     3481 cgcggcggcg acgaagatcg ccatcgtccg ctcgggcaac tactcgctgg gcgtcaacat
     3541 gctgatgggc ctggtgaaac gggccgccgc cgccctgccg gcgtccgagt gggacatcga
     3601 ggtcttcgag gcccaccaca agcgcaagat cgacgccccc tcgggcacgg ctctgatgct
     3661 gggccaggcc gccgccgacg gccggggcgt ggccctgtcc gacgtcgagg agcgcgcccg
     3721 cgacggcgtc accggggcgc gcacgcaggg cgcgatcggc ttttccgtgg tgcgcggcgg
     3781 cggcatcatc ggcgagcaca gcgtgatctt cgccggcgag gaagaggtgc tgaccctgtc
     3841 gcactcggcc accgaccggg gcctgttcgc gcgcggcgcg gtggcggcgg ctgtctgggc
     3901 cgtaggcaga ccgccgggtc tgtacgacat gcaggacgtt ctgggctttc gcgaataaac
     3961 gccgcgaact agacattggg ttgagccggg gggcttggaa ccagacctag gacctgatgg
     4021 ccgagccgac gcccgatccc gctcccccga ccagagcttt ccgccgtcga gaacggttga
     4081 tcgacgccgg gctggaagcc gtctcgctgt cgatgctggc cttcatcgcc gtcggcctgc
     4141 tggcgcacgg cggtcgccgc gagatcagcc gcgagctggc ccagagctgg ctgcgcgaac
     4201 gcggcatcga cgccgtggtg gtagtcgacg acctggacgc cagcggcttt tccggctcgg
     4261 tgctgctggg ctcaaagagc gacccgatct tcgccgccga ccgcatcgag gtcgcctatg
     4321 acctgacgac gccgtggaat ggcgacggct ttgctgtcga cacgcgcgcc gtgcgcctgg
     4381 tgcgcccccg tatcaaggcc agcctgaccg agcagggcgg cctgaacttt ggggcccttc
     4441 agcccctgat cgacgaggcc ctggcctcgc ccaaggatcc gaaggccacc ggcccggcca
     4501 tcctggtcga gaacgcgcgg ctggacctgc gcacccccgg cggcctggct cgcgtcaccg
     4561 gcgacgccag tctcgacaac ggcaagctgc tgcgcttcga cggccggctg gcgcggatgc
     4621 gctacgccac cggcgatctg gtgatcgagg cgcgcggcgc gctgctgtcg gcccgcaagc
     4681 gcggcgagcg cctgaccgtc gcggccagct ttgacatcga tgcgctggtc cagccggccc
     4741 tcgagctcga cgacgcccag ggccagatcg acgccgacct ggcctatccc gacctcgtca
     4801 aactgtcggc cacgggcccc gccgaggtgc ggctggcgct caaggccgcc tcggcccggg
     4861 ccggcgcgac ccggatcaac gacgcggcga cgaccctcgc cctgctcggc gtcctggacg
     4921 ggaccctgac ccagggccgg ttcgaagggc aggtgaccgg caaggcgcgg ggcgaaaccc
     4981 tgcggaccca gggtctggac gcccgtgaca tcagcctgga ccttaatctc acccaagtgg
     5041 ccgcctcgag gctgggcgag aagattgccg tccagacccg gggccacgtg caggccgagg
     5101 ccggccacgc cctggccagc ggcgcggccc tgcgcgaggt ttccgcgcga ctggacctgg
     5161 ccggtctgat cctgtcggcc gacaaggccg gcgctgtggt cagtggtccg ttggcgctgc
     5221 agctgggcgc gagccggctg gccagcggcg gcctggccct gaacagcctg gcggtcgagg
     5281 ccaggggccg cgtggccggc ccgctgaccc atcccatcct gaccgccagc ggctcggccg
     5341 gcgccgatag cggtgtcgcg ggtcccgacg ccgagcggct ggctgcagcg ctgcccgatc
     5401 cggccgatgc ccgcgccgtc gcccaggccc tgcagacctt cgacctgcgc gccccggcct
     5461 tcgacctgac cctgcgcgat ggccggacca ttctggccct gccccgcccg atcaccctga
     5521 ccgccgccaa tggcgtgcgg gccagcctcg ccgcgccgcg cgggccgctg ctggacgccc
     5581 tggccggcca gatgcg
//
LOCUS       DAOMEO010000039         5570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1895_length_5570_cov_2.586219, whole genome shotgun sequence.
ACCESSION   DAOMEO010000039 DAOMEO010000000
VERSION     DAOMEO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1895_length_5570_cov_2.586219"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLH31_01570"
     CDS             complement(<1..466)
                     /locus_tag="PLH31_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87969.1"
                     /translation="MLFVIAGVLGFITITALGFAFAGGDQNAARTAKRAQAIVGGGAR
                     DAGVRAKAVANTPDARRKQILKTLKEQDKQQKKASLTIAARLRAAGLGEKVQMFWIIS
                     GVLGLSIGLVILVLRQHPLIALGGAFAAGFGLPRWGNPGRYGRRDERDQHDAA"
     gene            complement(470..1975)
                     /locus_tag="PLH31_01575"
     CDS             complement(470..1975)
                     /locus_tag="PLH31_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HQR87970.1"
                     /translation="MFGKRDQNPATDRQAPPPAPVDGSAIATRPQRVEPAADRGGERS
                     APSPKVAAPKATPGLEQLRAAQGAPATTHIVREQSDYYHATKTVIFNALLNTIDLSQL
                     AQLDLKAAAEEIRDIVAELVAIKNVSMSVSEQEHLVQDIINDVLGYGPLEPLLARDDI
                     ADIMVNGAQRVFIEVGGKVQLTNVRFRDNLQLMNICQRIVSQVGRRVDESSPICDDRL
                     PDGSRVNVIAPPPALDGPTLTIRKFKKDKLTMKNLVDYASISPEGARVLGVIGACRCN
                     IVISGGTGSGKTTLLNTLTAFIDPTERVVTCEDAAELQLQQPHVVRLETRPPNLEGQG
                     AVTMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREAISR
                     IESMITMGGYGLPSKTIKEMIVGSVDVIVQAARLRDGSRRITHITEVVGLEGDVIVTQ
                     DLFVYEITGEDADGKVIGKHRSTGIARPRFWDRARYYGLERELAEALDAAE"
     gene            complement(1982..3565)
                     /locus_tag="PLH31_01580"
     CDS             complement(1982..3565)
                     /locus_tag="PLH31_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE family protein"
                     /protein_id="HQR87971.1"
                     /translation="MARPPSDHDPFDLGFEAADEFTSAGADPWRSSAPALTPAMGGLD
                     DPFADFPPARPRDERPSAAQDPFAAPAAARQAQVQTAAPARAPAPAPQPQPEVQRAPA
                     AEAALAAAPEPFAPILHDGLTGSDAALGEMAVPRITIHAFCVRPETAQMIEQAAADRR
                     MARASTIVRDGGLDAAVELYQNQSTPSLVLVESHDGAQRLLHLLDALAQVCDPGTKVV
                     VIGQANDIALYRELMRRGVSEYLTQPLGPLQIIRAVSGLYADPSAPFVGRQVAFVGAK
                     GGVGASTLAHNFAWSMAERMQTATVIVDLDLPFGTAGLDFNQDPLQGVIDALTQPERL
                     DPVLMDRMMVRCGDRLSLFAAPATLDQDYEISAEAYEEVTQKIRGAAPFVVLDLPHSW
                     AAWTRRVLIGSDDLVVVATPDLASLRNAKNIVDMVRQARPNDAPPRLVLNQVGVPGRP
                     EIPVKDFGEALGVTPSLVLPFDPKSFGQAANNGQMVGEVAPKSKAAEGIDHLAQLISR
                     REPPQVQKASMLAGLFRKK"
     gene            complement(3565..>3842)
                     /locus_tag="PLH31_01585"
     CDS             complement(3565..>3842)
                     /locus_tag="PLH31_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HQR87972.1"
                     /translation="FEIFTAEGPTCGRWENLTATRKNEAYGNFGCAVAANLAAQIANP
                     EDLIRPRDSTPIDAGRRDTVVGKYRKGEVTSSAKDDQANGAISKAIN"
     assembly_gap    3843..3852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3853..4272)
                     /locus_tag="PLH31_01590"
     CDS             complement(<3853..4272)
                     /locus_tag="PLH31_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HQR87973.1"
                     /translation="MNKPPVKSVLLLGAALLTLSACASARVEPPVPAPVSRTDSEKWR
                     DQIKVDGRADEIQLAVHATGVSANQDQALSALVSRWLSAQAREIVVSAPVGGGDAAGG
                     MAVQVRERLVFYGAAPGQVRVVGYDAIGLDRAPLRVGF"
     gene            complement(4283..>5570)
                     /locus_tag="PLH31_01595"
     CDS             complement(4283..>5570)
                     /locus_tag="PLH31_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HQR87974.1"
                     /translation="EVRIVEVQRNVIKQLGVDLNAVLGQLGSDQFSFGLSPTYGVNGS
                     LLGGVRGGYNVNTTSQPTNLIPSLFGAVPGAQSGVSLLNYLQRFLSNDSSLSSAQSSW
                     LGSYANSIAGKTTAVVGTGANAVTYTMKDVGISAASLSSDVQSFLAGGSGTAGNDAKN
                     LWIQNFLTNVNTVTPGSGFYQDKGNPLASVTNGLAGSDDVNSAKAMIQAFERVGLVRT
                     LAEPNLTVLSGEAGKFLAGGEFPVPVAADNTGKITIEFKPYGVGLGYTPVVLSGGRIS
                     LKLSTEVSELSSLGAYSVTTASSVLVVPGLSVRRAETTVELPSGGSLMIAGLLQQTTK
                     QTIDAIPGMTSMPVLGALFRSRDYLNGETELVIIITPYIVDPTRPQNLQTPADGLKFA
                     GDMSTALLGRLNQVVNAPAGANAGRAYQGPVGYVIE"
BASE COUNT          849 a         1810 c         1987 g          914 t
ORIGIN      
        1 cagcggcgtc atgttgatct cgttcatcac gtcgtccgta tcgtcctggg ttcccccagc
       61 gcggcaggcc aaagccggcc gcgaaggcgc cgcccagggc gatcagcgga tgctggcgca
      121 ggaccaggat caccaggccg atgctgagac ccagcacgcc gctgatgatc cagaacatct
      181 ggaccttttc gcccaggccg gcggcgcgca ggcgcgcggc gatggtcagc gaggccttct
      241 tctgctgctt gtcctgctct ttgagggtct tgaggatctg cttgcggcgg gcgtcgggcg
      301 tgttggccac ggccttggcg cggacgccgg cgtcgcgggc tccgccgccg acaatggcct
      361 gggcgcgctt ggcggtgcgg gccgcgttct ggtcgccgcc ggcgaaggcg aaacccaggg
      421 cggtgatggt gatgaagccc agaacgccgg cgatcacgaa cagcatcgct tactccgccg
      481 cgtcgagggc ttcggccagc tcgcgttcca gaccgtaata gcgggcgcgg tcccagaagc
      541 ggggccgggc gatgccggtc gagcggtgct tgccgatcac cttgccgtcg gcgtcctcgc
      601 cggtgatctc gtagacgaac aggtcctggg tgacgatcac gtcgccttcc aggcccacca
      661 cctcggtgat gtgggtgatc cggcgcgagc cgtcgcgcag acgggcggcc tggacgatga
      721 cgtcgaccga gccgacgatc atctccttga tggtcttcga gggcaggcca tagccgccca
      781 tggtgatcat gctctcgatc cggctgatgg cttcgcgcgg gctgttggcg tgcagcgtgc
      841 ccatcgagcc gtcgtggccg gtgttcatgg cctgcagcag gtcgaacgcc tcagggcccc
      901 ggacctcgcc gacgatgatg cgctcgggac gcatccgcag acagttcttg accagatcgc
      961 gcatggtcac cgcgccctgg ccttccaggt tgggcggccg ggtttccagg cgcaccacgt
     1021 gcggctgttg cagctgcagt tcggcggcgt cctcgcaggt caccacccgc tcggtcgggt
     1081 cgataaaggc ggtcagggta ttgagcaggg tggtcttgcc agagcccgta ccgccggaaa
     1141 tgacgatgtt gcagcggcag gcgccgatga cgcccaggac ccgcgcccct tccggactga
     1201 tcgacgcata gtcgaccagg ttcttcatcg tcagcttgtc cttcttgaac ttccggatgg
     1261 tcagggtggg accatccaga gccggagggg gcgcaatgac attgacgcga ctgccgtcgg
     1321 gcaggcggtc gtcgcagatc ggcgaacttt catcgacccg gcggccgacc tggctgacga
     1381 tccgctggca gatgttcatc agctgaagat tgtcgcggaa gcgcacattg gtcagctgga
     1441 ccttgccgcc gacttcgatg aacacccgct gggcgccatt gaccatgatg tcggcgatgt
     1501 cgtcgcgggc cagcaggggc tccagcgggc cgtagcccag gacgtcgttg atgatgtcct
     1561 ggaccaggtg ctcctgctcg gagaccgaca tcgaaacgtt cttgatcgcc accagctcgg
     1621 cgacgatgtc gcggatctct tcggcggccg ccttcaggtc cagctgggcc agctggctca
     1681 ggtcgatggt gttgagcagc gcgttgaaga tgaccgtctt ggtggcgtgg tagtagtcgc
     1741 tctgctcacg cacgatatgg gtggtggccg gagcgccctg ggcggcgcgc agctgttcca
     1801 ggcccggcgt cgccttggga gccgccacct tgggcgacgg cgcgctgcgc tcgcccccac
     1861 ggtcggcggc cggctcgacc cgctgcggac gcgtggcgat cgccgacccg tcgacgggcg
     1921 ccggaggcgg cgcctgacgg tcggttgcgg ggttctgatc gcgtttgccg aacatccgtc
     1981 gctatttctt cctgaacaga ccagccagca tcgaggcctt ctgaacctgc ggcggttcgc
     2041 gacggctgat cagctgggcc aggtggtcga tgccctcggc cgccttggac ttgggcgcca
     2101 cctcgccgac catctggccg ttgttggcgg cctggccgaa ggacttggga tcgaagggca
     2161 ggacgagcga gggcgtcacg cccagggcct cgccaaaatc cttgaccggg atttcgggac
     2221 ggccgggcac gcccacctgg ttcagcacca ggcgcggcgg ggcgtcattg gggcgggcct
     2281 gacgcaccat gtcgacgatg ttcttggcgt tgcgcaagct ggccaggtcc ggcgtggcga
     2341 ccacgaccag gtcgtcagag ccgatcagca ccctgcgcgt ccaggccgcc cagctgtgcg
     2401 gcaggtccag caccacgaag ggcgcggcgc cgcggatctt ctgggtgacc tcttcatagg
     2461 cctcggcgct gatctcgtag tcctggtcca gggtggccgg ggccgcaaac agcgacaggc
     2521 gatccccgca acggaccatc atccggtcca tcagcaccgg atccaggcgc tcgggctggg
     2581 tcagggcgtc gatcacgccc tgcagcgggt cctggttaaa gtcgaggccg gcggtgccga
     2641 acggcagatc caggtcgacg atcaccgtgg cggtctgcat gcgctcggcc atcgaccagg
     2701 cgaaattgtg ggccagggtc gaggccccga ccccgccctt ggcgccgaca aaggccacct
     2761 ggcggcccac gaagggcgcc gacggatcgg cgtaaagacc agacaccgcg cggatgatct
     2821 gcagcgggcc cagcggctgg gtcagatatt cgctcacccc ccgccgcatc agctcgcgat
     2881 agagggcgat gtcgttggcc tggccgatca caaccacctt ggtgcccggg tcgcagacct
     2941 gggccagggc gtccagcagg tgcagcaggc gctgggcgcc gtcatggctt tccaccaaca
     3001 ccagcgaggg cgtcgactgg ttctgataga gctcgacggc ggcgtccagg ccgccatcgc
     3061 gcacgatggt cgaggcgcgg gccatgcggc ggtcggcggc ggcctgctcg atcatctggg
     3121 cggtttcggg ccgcacgcag aaggcgtgga tggtgatgcg cggcaccgcc atctcgccca
     3181 gggcggcgtc gctgccggtc aggccgtcat gcaggatcgg ggcgaacggc tcgggcgccg
     3241 cagcaagggc cgcctcggct gcaggcgcgc gctggacctc gggctggggc tggggcgcgg
     3301 gcgcgggcgc gcgggccggc gctgccgtct ggacctgggc ctgccgcgcg gcggccgggg
     3361 ccgcgaaggg atcctgcgcg gccgagggtc gttcgtcgcg cggccgggcc ggggggaagt
     3421 cggcgaaggg gtcgtccagc ccgcccatgg ccggcgtcag ggccggggcg gacgagcgcc
     3481 agggatcggc gccggccgag gtgaactcgt cggcggcctc gaagcccagg tcgaacgggt
     3541 cgtgatccga aggaggacgc gccatcagtt gatcgccttc gaaatggcgc cgttggcctg
     3601 gtcgtccttg gccgaggagg tgacctcgcc cttgcggtac ttgccgacga cggtgtcgcg
     3661 gcggcccgca tcgatcggcg tggagtcgcg cggacggatc aggtcttcgg gattggcgat
     3721 ctgggcggcc aggttggcgg ccacggcgca gccgaaattg ccataggcct cgttcttgcg
     3781 cgtggcggtg aggttttccc agcggccgca ggtcggtccc tcggcggtga agatctcgaa
     3841 acnnnnnnnn nngaaaccga cccgcagggg cgctcggtcc aggccaatgg cgtcatagcc
     3901 caccacccga acctggcccg gggccgcgcc atagaacacc agacgttcgc gaacctgcac
     3961 ggccatgccg ccagccgcgt cgccgccgcc aacgggggcg ctgacgacga tctcgcgggc
     4021 ctgggccgac agccagcggc tgacgagggc cgacagggcc tgatcctgat tggccgagac
     4081 gccggtggcg tgaacggcca gctggatttc gtccgcgcgg ccgtcgacct tgatctggtc
     4141 gcgccacttt tcgctgtcgg tgcgcgagac gggcgcagga accggcggtt caacccgggc
     4201 gctggcgcag gccgacaggg tcagcagggc cgcgccgagg agcaggacgg atttgacggg
     4261 gggcttgttc atgtccgcgt cctcactcga tcacatagcc gacggggccc tgataggcgc
     4321 gccccgcatt ggcgccggcc ggcgcattga ccacctggtt gagccggccc agcagggcgg
     4381 tgctcatgtc gccggcgaac ttgaggccgt cggccggggt ctgcaggttc tggggccggg
     4441 tgggatcgac gatgtagggg gtgatgatga tcaccagctc ggtctcgccg ttcaaatagt
     4501 cgcgcgaccg gaacagcgcg cccagcaccg gcatcgaggt catgccgggg atcgcgtcga
     4561 tcgtctgctt ggtggtctgc tgcagcagac cggcgatcat cagcgatccg cccgagggca
     4621 gctcgaccgt ggtctccgct cggcggacgg aaagacccgg caccaccagg acgctgctag
     4681 cggtcgtcac cgaataggcg cccaggctgc taagctccga cacttcggtc gacagcttca
     4741 gcgagatgcg gccacccgac agcacgaccg gcgtatagcc caggccaacc ccgtagggtt
     4801 tgaactcgat ggtgatcttg ccagtattgt cggccgcgac cggcaccgga aactcgccgc
     4861 cggccaggaa cttgcctgcc tcgcccgaca gcacggtcag gtttggctcg gccagggtgc
     4921 ggacaaggcc tacgcgctca aacgcctgga tcatcgcctt ggcggaattg acgtcgtcgc
     4981 tgccggccag cccgttggtg acgctggcca gggggttgcc cttgtcctga tagaagcccg
     5041 agcccggcgt gaccgtgttc acattggtca ggaaattctg gatccacagg ttcttggcgt
     5101 cgtttccggc tgtgccggaa ccgccggcga ggaagctctg cacatcggat gacagcgagg
     5161 ccgccgaaat cccgacgtcc ttcatggtat aggtcacggc gttggcgccc gtcccgacga
     5221 cggcggtagt cttgccggcg atagaattgg cgtaggagcc cagccacgag ctctgggccg
     5281 agctcaggct gctgtcattg gacaggaagc gctggagata attcagcagc gacacgcccg
     5341 actgagcgcc tgggaccgcg ccgaacaggc tggggatcag attggtcggc tggctggtgg
     5401 tgttgacatt atagccgccc cggacgcccc ccagcaggct gccgttgacg ccataggtcg
     5461 gcgagagtcc aaacgagaac tggtcgctgc ccaactggcc gagcacggcg ttcagatcga
     5521 cgcccagctg cttgatgaca ttgcgctgca cctcgacgat gcgcacttcc
//
LOCUS       DAOMEO010000040         5530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1904_length_5530_cov_2.659178, whole genome shotgun sequence.
ACCESSION   DAOMEO010000040 DAOMEO010000000
VERSION     DAOMEO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1904_length_5530_cov_2.659178"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLH31_01600"
     CDS             <1..191
                     /locus_tag="PLH31_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQR87975.1"
                     /translation="VNGPLTHPLYAFLKKEQKGLLGTEGIKWNFTKFLIGRDGEVVER
                     FAPTTKPEDLKVAVEALL"
     gene            274..1674
                     /gene="lpdA"
                     /locus_tag="PLH31_01605"
     CDS             274..1674
                     /gene="lpdA"
                     /locus_tag="PLH31_01605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQR87976.1"
                     /translation="MSTEFDVVVIGAGPGGYVAAIRASQLGLKTAIVERENLGGICLN
                     WGCIPTKALLKSGEIFEQLSHLDAYGLSVEKPAFDFTKIIERSRGVAKTMSGGIAFLM
                     KKHKIEVIEGEARLEKGSPAPKVVVALKAGGSRAIQAKNVILASGARARNIPAIGAVS
                     DGDKVWTYRDALAPKTMPKSLVVIGSGAIGIEFASFYRALGAEVTVVEAVDRIMPVED
                     AEVSKAAQKAFEKRGIKFRIGAKVTKIDKTGTGVSVSVEVGGKAEQLTAEKCIVAIGI
                     VPNTEGTEAVGLTTDRGHVVTGKHGETNVPGLYAIGDVAGAPWLAHKASHEGIHAAEH
                     IAGYKTPAVHSPIPGCTYANPQVASVGYTEAAAKAAGIDVKAGRFPFKVNGKAVAAGE
                     TEGFVKVVFDGKTGALIGAHMIGHEVTEMIQGFVTAITLEATEEDLHGIVYAHPTMSE
                     AMHEATLDAYGRVLHI"
     gene            1683..3188
                     /locus_tag="PLH31_01610"
     CDS             1683..3188
                     /locus_tag="PLH31_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQR87977.1"
                     /translation="MARATATETDKSAKALAKTSAKTRKGAKTTLSEANLADLGAERL
                     ASILMELSTDAYVKRALRLELFAEVSPEGLALEITKRLAAIAKATSRIHWRKRAAFAR
                     DLELHLRMIQRLAKTDPKTALPLLMDFLDLAGSVFDRISQPEGPIGEVFAEARDTFGD
                     IAPAARIAPVDLADRVARLILCDDHFVFGPLVAVAAPSLGPAGLARLGERLQAALADR
                     PARSAGAYDAKASVVRDALRRLADISGDVGGFIATFSETQRRFPAIAAEIGRRLLAAG
                     KAREALAALDIAAPNLKPRSLLSSRAAEPDDPVDRAVWEDVYLDALQANGRQDEAQRR
                     RWAAFEATLSIPRLKAFLKGLPDFDDVVAEDDAKKIATEFPRFDTALAFLIEWPDLPA
                     ASRLIVNRHGAINGADEELLTAAARRLEGAYPLAATLLLRAMIRNTLTYGIAGRYKAA
                     AQQWLEVESLAPMIADFGEFPDHETFMADLRATHGRKQGFRAELEALGGVF"
     gene            complement(3198..4751)
                     /locus_tag="PLH31_01615"
     CDS             complement(3198..4751)
                     /locus_tag="PLH31_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR87978.1"
                     /translation="MPVVFNAEDGQKGLFDALIDARDTYCAKRPILEDQDRNPLSYTD
                     LIRASFALGRKIASMTKPGEHVGVLLPSGSAAVVTFFALHAFGRVPTMLNFTAGLRNL
                     KAACALAKVQRVLTAHKFIELGNLHDIVDALESQTKITYLEDVRSAIGVTDKLFAAAA
                     GLFPRQFRAQAKPSDKGVVLFTSGSFGAPRGVVLSQANLVSNAEQIAAHIALDPDWVF
                     FNPLPVFHCFGLTGGVILPLLNGMKAFQYPSPLHTKQIPALVKDTGASVLFATDTFLN
                     HYARASDSDELSGLRFVVCGAEKVREETHNLIRERFGDVPVLEGYGATEASPVIAVNK
                     PEDNRRGTVGGLLPGQEFRIEPVEGIVEGGRLLVRGPNIMSGYLREDGGVDAPEGGWH
                     DTGDVVVMSEDNWITIKGRVKRFAKIGGEMVSLTAAEDLASAVWPDGRHAVIAMPDKK
                     KGEKLVLVTDRHDAAVSDLVAHAQTIGAPELSVPRKILKVPEVPVLGSGKTDYVAIQR
                     MADQDAKAA"
     gene            complement(4853..>5075)
                     /locus_tag="PLH31_01620"
     CDS             complement(4853..>5075)
                     /locus_tag="PLH31_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87979.1"
                     /translation="APVLLLRGGEDRLTASARATSACVAFADCRNVTFPGGRHELHME
                     SDAVREAWLGQVEAFVRARILAKAPRHKG"
     assembly_gap    5076..5085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5086..>5530)
                     /locus_tag="PLH31_01625"
     CDS             complement(<5086..>5530)
                     /locus_tag="PLH31_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87980.1"
                     /translation="ETDAAALRALVKVVIRPSGETPLVVLGHSDGAVVALRAVQTGLP
                     TDGLMLSSPAFALNNLPHLPTDPRQTTLWASRLKLGWLKAPGAPGWRRDGQGSVALGL
                     TSDRLRGAVQQAWLTANPDLRIGAPSLGWMAALYEAADRAQAEAAR"
BASE COUNT          969 a         1825 c         1804 g          922 t
ORIGIN      
        1 acgtcaacgg ccccttgacc catccgctct atgccttcct gaagaaggag cagaagggtc
       61 tgctgggcac cgagggcatc aagtggaact tcaccaagtt cctgatcggc cgcgacggcg
      121 aagtcgtcga gcggtttgct cccaccacca agcccgaaga cctcaaggtc gcggtcgagg
      181 cgctgctcta attctcccgg atcggcttgc aggccgatcg atcgaggccg cccggctccc
      241 ctcgggcgac gtccaagcta gggtaagaac accatgtcca cggaattcga tgtcgtcgtt
      301 atcggcgctg gtcctggcgg ctatgtggcc gcgatccgcg ccagccagct tggcctgaag
      361 acggcgatcg tcgagcgcga gaatctgggt ggcatctgcc tgaactgggg ctgcatcccc
      421 accaaggctc tgctgaagtc cggcgagatc ttcgagcaac tgtcgcacct ggacgcctac
      481 ggcctgtcgg tggaaaagcc ggccttcgac ttcaccaaga tcatcgagcg ctcgcgcggc
      541 gtggccaaga ccatgtcggg cggcatcgcc ttcctgatga agaagcacaa gatcgaggtc
      601 attgagggcg aagccaggct cgaaaagggt tctcccgctc ccaaggttgt cgtggccctg
      661 aaggccggtg gcagccgcgc gatccaggct aagaacgtca tcctggccag cggcgcgcgg
      721 gcccggaaca ttcccgccat cggcgcggtg tcggacggcg acaaggtctg gacctatcgt
      781 gacgcccttg cccccaagac catgccaaag tcgctggtcg tcatcggctc gggcgccatc
      841 ggcatcgagt tcgcgagttt ctatcgcgcc cttggcgccg aggtgaccgt cgtcgaggcc
      901 gtcgaccgca tcatgccggt cgaggacgcc gaggtgtcca aggccgccca gaaggcgttc
      961 gagaagcgcg gcatcaagtt ccgtatcggc gccaaggtga ctaagatcga caagaccggc
     1021 acgggcgtct cggtttcggt cgaggtcggc ggcaaggccg agcaactgac cgccgagaag
     1081 tgcatcgtcg ccatcggcat tgtgcccaat actgaaggca cggaagccgt gggcctgacc
     1141 accgatcgcg gtcatgtggt caccggcaag cacggcgaga cgaacgttcc gggcctctac
     1201 gccatcggtg acgtcgccgg cgcgccctgg ctcgctcaca aggccagcca tgaaggcatc
     1261 cacgcggccg aacacatcgc cggctacaag acgcccgccg tgcactcgcc gatcccgggc
     1321 tgcacctatg ccaacccgca agtcgcctcg gtgggctaca ccgaagctgc cgccaaggcg
     1381 gccggcattg acgtaaaagc cggccgtttc ccgttcaagg tcaatggcaa ggccgtggcc
     1441 gccggcgaga ccgagggctt cgtcaaggtg gtgttcgacg gcaagaccgg cgccctgatc
     1501 ggcgcccaca tgatcggcca cgaagtgacc gagatgatcc agggtttcgt taccgccatc
     1561 acgctggaag cgaccgagga agacctgcac ggaatcgtct acgctcaccc gaccatgtcg
     1621 gaagccatgc acgaagccac gctcgatgcg tatgggcggg tgctgcacat ctaggggcct
     1681 gaatggcgcg cgcgacggcc acagagaccg acaagtcagc caaggctttg gccaaaactt
     1741 cggccaagac tcggaagggg gccaagacca ccctttccga agccaatctg gccgatctgg
     1801 gcgcggaacg gctggcgtcc attctgatgg agctatcgac cgacgcctac gtcaagcgcg
     1861 ccctgcggct ggaactgttc gccgaggtca gtcccgaggg gctggcgctg gagatcacca
     1921 agcgtcttgc ggcgatcgcc aaggcgacct cgcggattca ctggcgcaaa cgcgcggctt
     1981 tcgcgcgcga tctcgaactt catctgcgca tgatccagcg cctggccaag accgatccca
     2041 agacggcgct gccactactg atggacttcc tggatctggc gggttcggtc tttgaccgga
     2101 tcagccagcc cgaaggtccg atcggcgagg ttttcgctga ggcgcgcgac accttcggtg
     2161 acatcgcccc ggccgccagg atcgcgccgg tcgatctggc tgacagggtg gcgcggctga
     2221 tcctttgtga tgaccacttc gtctttggcc ctctggtcgc ggtcgcggcg ccgtcgctgg
     2281 gtcctgcggg gctggcgcga ctgggcgagc gcctgcaagc agccttggcc gaccgtcccg
     2341 cacgctcggc aggggcctat gacgccaagg cgtccgtggt gcgcgacgcc ctgcgccgcc
     2401 tcgccgacat cagcggcgat gtcggcggct ttatcgcgac cttcagcgag acgcagcgtc
     2461 ggtttcccgc catcgccgcc gagatcgggc gccggctctt ggccgcagga aaagcgcgcg
     2521 aggccttggc cgcattggat attgccgcgc ccaacctcaa gccgcgctca ctgctttcgt
     2581 cccgcgcggc cgagcctgac gatccggtcg atcgggccgt gtgggaagat gtctatctcg
     2641 acgcgctgca ggccaatggc cgccaggacg aggcccagcg ccggcgctgg gcggctttcg
     2701 aggccaccct gtccatcccg agactgaagg cgtttctcaa gggcctgccg gacttcgatg
     2761 atgtggtggc cgaggacgac gccaagaaga tcgccaccga gtttccgcgc tttgacacgg
     2821 ccctggcctt tctgatcgaa tggccggatc tgccggcggc gtcgcggctg atcgtcaacc
     2881 ggcacggcgc catcaacggc gcggacgaag agctgctgac cgccgccgcg cgccgtctgg
     2941 aaggcgccta tcccctggcc gcgaccctgc tgctacgcgc catgatccgc aacacgctga
     3001 cctatgggat cgcgggccgc tacaaggccg cagcccaaca gtggcttgag gtggagtccc
     3061 tggccccgat gatcgccgac ttcggtgagt ttcccgacca tgagaccttc atggctgacc
     3121 tgcgggcgac gcatggccgc aaacagggct tccgggccga gctggaagcc ctgggcggcg
     3181 tcttttagcg cgtcagtcta ggcggccttg gcgtcctgat cagccatgcg ctggatagcg
     3241 acatagtcgg tcttgccgct gcccaggacg ggcacctccg gcaccttcag gatcttgcgc
     3301 ggcaccgaca gctccggcgc gccgatcgtc tgggcgtggg ccacaaggtc ggacaccgcc
     3361 gcatcgtgac gatcggtgac cagcacaagc ttctcgcctt ttttcttgtc aggcatggca
     3421 atcacagcgt ggcggccatc gggccagacg gccgaagcca gatcctcggc ggctgttagc
     3481 gaaaccattt cgccgccgat cttggcgaag cgcttcacgc gacccttgat ggtgatccag
     3541 ttgtcttccg acatcacgac cacgtcgccg gtgtcgtgcc agccgccttc cggagcgtcg
     3601 acgccgccgt cctcgcgcag atagcccgac atgatgttgg gcccccgcac cagcaggcgt
     3661 ccgccctcga ctatgccctc gacgggttcg atgcggaact cttggccggg cagcaagccg
     3721 cccaccgtgc cgcgccggtt gtcttcgggc ttgttgaccg cgatcaccgg cgaggcctcg
     3781 gtcgcgccat agccttccag aacggggacg tcgccaaacc tctctcggat gaggttgtgc
     3841 gtttcttccc taactttttc agcgccgcaa acgacaaacc tcaggccgga aagctcgtcg
     3901 ctgtcggacg cgcgcgcata gtggttcagg aaggtgtcgg tcgcgaaaag caccgaggcg
     3961 ccagtgtcct tgaccagggc cgggatctgc ttggtatgca gcggcgaggg atactggaaa
     4021 gccttcatgc cattcaacag cggcaggatc accccgcccg tcaggccaaa gcagtggaac
     4081 acggggaggg ggttgaagaa cacccagtcg gggtccagcg cgatgtgggc ggcgatctgc
     4141 tcggcattgc tgaccaggtt ggcctgactc aacacgaccc cgcggggcgc cccgaagctg
     4201 ccggacgtga agagcacgac ccccttgtct gaaggcttgg cctgggcgcg gaactggcgc
     4261 gggaatagcc ccgccgcggc cgcgaacagc ttgtcggtca cgccgatcgc gctgcgcacg
     4321 tcctccaaat aggtaatctt cgtctggctc tccagagcgt cgacgatgtc gtgcagattg
     4381 cccagttcga tgaacttgtg agcggtcagc acccgctgga ccttggccag ggcgcaggcg
     4441 gccttcaggt ttcgcagacc cgccgtgaag ttgagcatgg tcggcacgcg gccaaaggcg
     4501 tgcagggcga agaaggtcac cacggcggcc gagcccgagg ggagcagcac gccgacatgt
     4561 tcgccaggct tggtcatgga cgcgatcttg cggcccagcg cgaagctggc ccggatcagg
     4621 tcggtgtagc taagcggatt tctgtcctga tcctcaagga tgggcctctt ggcgcaatac
     4681 gtgtcgcgcg catctataag ggcgtcaaat agtccctttt ggccatcttc cgcattaaaa
     4741 actaccggca ttccggcgga acctccccga agagcgacat cgctttcgtt cgttgagccc
     4801 gagactgccg gggaaggtcc ggctttgcaa ggaagagtca cgttacgcta ggtcagccct
     4861 tgtggcgtgg ggccttggcc aggatccgcg cgcgaacaaa ggcctcgacc tggcccagcc
     4921 aggcctctcg cacagcgtct gattccatat gaagctcatg gcgtccgccc gggaatgtca
     4981 cgtttcggca gtcggcgaag gcgacacagg cggaggttgc gcgggccgag gcggtcaggc
     5041 ggtcctcgcc gccccgcaac agcaggaccg gcgccnnnnn nnnnncgggc cgcctcagcc
     5101 tgcgcgcgat cggcggcttc gtagagggcg gccatccagc ccagcgacgg cgcgcctatc
     5161 cgcaaatcag gatttgccgt cagccaggcc tgctgcaccg cgccgcgaag ccggtcggag
     5221 gttaagccga gcgccacgct accctggcca tcgcggcgcc agcccggcgc gcccggcgct
     5281 ttcagccagc ccagcttcag ccggctggcc cagagcgtgg tctggcgcgg gtcggtcgga
     5341 agatgaggca ggttattcag ggcgaacgcc ggagaggaca gcatcaggcc atccgttggg
     5401 aggccggtct gaaccgctct gagggccacc acggcgccat cggaatggcc aagaacgacc
     5461 aacggcgtct cgccgctggg ccggatcacc accttgacca gggcgcgcag ggcggcggcg
     5521 tcggtctcga
//
LOCUS       DAOMEO010000041         5523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1907_length_5523_cov_2.394477, whole genome shotgun sequence.
ACCESSION   DAOMEO010000041 DAOMEO010000000
VERSION     DAOMEO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_5523_cov_2.394477"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLH31_01630"
     CDS             complement(<1..332)
                     /locus_tag="PLH31_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR87981.1"
                     /translation="MTESPEFLVRADNQRIAYRRVDGEGPTVVWLGGFHSDMTGTKAQ
                     VLADHAQATGGAYLRFDYFGHGQSDGQFRDGTISRWREDALAVIDELTEGPLVLVGSS
                     MGGWIACLA"
     assembly_gap    373..382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            393..1574
                     /locus_tag="PLH31_01635"
     CDS             393..1574
                     /locus_tag="PLH31_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR87982.1"
                     /translation="MPALPADFTLLQVTPELETGGAEQTTIDVAHAVVAQGGKALVAT
                     KGGRMASRLEADGGRLAQMPAQSKNPLVMLGNAARLIDLIRREKVSLVHARSRAPAFS
                     ALWAAHATGTPFVATYHGVYNARSNLKRWYNAVMTKGDLVIANSEYTRAHVIKEHGID
                     PARVVSIPRGVDLTRFEPGMVGGDRIAALRQAWGVAPDDRRLKVLLAGRLTRWKGQAL
                     LIEAMANLKARGEARVLLLLVGDDQGRKAYRAELEAQIAAADLQDDVRIVGHCDDMPA
                     AYLLADLAIAPSIEPEAFGRTAVEPQVMGRPVLASDHGAARETVLPGQTGWLVAPGDA
                     QAWADALATARDAGAATRQTMGQTARARARRLYSVDAMCEATLQVYARVLEARVLERR
                     P"
     gene            1571..2539
                     /locus_tag="PLH31_01640"
     CDS             1571..2539
                     /locus_tag="PLH31_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQR87983.1"
                     /translation="MSKEIKKVLVIKLGALGDFVLALAAMKKIREAHPKAKITLLTTP
                     PFEALAKLSPYFNSVETDGRPEDFGDLTQMLGRLRKARYDRVYDLQTNSRTNWYFQAL
                     RPFPPQWSGIAFGCSLPQRNKARQTMHTLERQADQLRAAGIWPDAPVEHGNAPPPDLS
                     WILRRHKEARPVAGAAAPRPYVLFVPGGSAHRPEKRWPVEAFAQLGSLLKARGLDIVI
                     IGGPQESAMARAIQKSVGQARDLTGRTDFAQLAVLGAKAALVVGNDTGPTHLLAAAGA
                     PTIALFSDASDPDLCGPRGHVAVIRSPDLKALPVSTVASAAISLLP"
     gene            2745..3266
                     /gene="infC"
                     /locus_tag="PLH31_01645"
     CDS             2745..3266
                     /gene="infC"
                     /locus_tag="PLH31_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQR87984.1"
                     /translation="MQTPPVKDGPRINDEIRVPRVLLIDQNGEKQGEMPTASALEAAE
                     EAGLDLVEIVPNANPPVCKILDYGKFKFQEQKKKNEARKRQKIVELKEIKLRPNIDIH
                     DYEVKTKAMTRFFEEGDKVKVTLRFRGREMAHPELGMKLLIKVKTDFEEIAKVEYEPR
                     MEGRQMIMILAPR"
     gene            3418..4629
                     /locus_tag="PLH31_01650"
     CDS             3418..4629
                     /locus_tag="PLH31_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR87985.1"
                     /translation="MSEPLPAAAPAHSNRALYVLLLVVFINLVGFGLVIPLLPFYAQS
                     LKAEPWQITALFSAYSLGQFFAEPFWGRLSDRIGRRPVLIVTILANTLSYVALAFAPT
                     IWIAFAIRLIGGFGSGNISTIQGYMADVTPPEQRAARMGLLGAAFGAGFVIGPALGGL
                     LAHPEAGRLGFQIPLFVAAGLAALASLGVFLFVVESRSPSAPDALRPHRREALQAAMA
                     HPVLSRVLLVTLVSTAAFAGMESVFGLWTHARFDWGPREVGLCFAVIGVIASIGQGAV
                     TGRLARRFGEPRVLTAGLSIIAFSLALTPFAPSGAAVPLFVGLTAFGQSLVFPCVAAL
                     ISRATPPDQQGAMLGLNMAAGSLARMAGPLLAGPLFGLALGGPYWFGALLTLPAIWLG
                     ASLVRRARAPA"
     gene            4693..>5523
                     /locus_tag="PLH31_01655"
     CDS             4693..>5523
                     /locus_tag="PLH31_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQR87986.1"
                     /translation="MLKMFAGAALAALIGMGVAQAAPLEAYGRLPAMSSVDISSDGQK
                     LAYIRTDGERSTIVVQGVDGTVLQALEMGARKVRDVQWTDNNHVLIRITETTTVSGIF
                     YAGEFALGASLNVTTGNATSLPSRTATRVANALFGGRGGTYNGKPVLYAGLFASTDGP
                     SFQHKDGSLDLYRIDPDSGIGQLHQRGDYTTGQYLFKSDGEVLARTQYQSEDGRWSLD
                     LRQNGWREAYSVIAPIDTPDLIGRSLDETSIILNVWDDKERIAFTFFLQSGYALEDTL
                     V"
BASE COUNT          883 a         1948 c         1754 g          928 t
ORIGIN      
        1 gccaggcagg cgatccagcc gcccatggac gagcccacca gcaccagagg cccctcggtg
       61 agctcgtcga tcaccgccag ggcgtcctcg cgccaccggc tgatcgtgcc gtcccggaac
      121 tggccgtccg actggccatg cccgaaatag tcgaagcgca gataggcgcc gcccgtggcc
      181 tgcgcatgat cggccagaac ctgggccttg gtcccggtca tgtccgaatg gaacccaccc
      241 agccagacaa cagtcggccc ctcgccatcc acgcgtcgat aggcgatgcg ctgattgtcg
      301 gcgcgaacca ggaattccgg ggattcggtc atggcgcggc cttgatcctt tcgctaacta
      361 aacccgtaat ctnnnnnnnn nncgagagtc gcgtgcccgc acttcccgcc gatttcacct
      421 tgctgcaggt cacccccgaa cttgaaacgg gcggcgccga gcagacgacg atagatgtcg
      481 ctcacgccgt cgtggcgcaa ggcggcaagg cgttggtggc gaccaagggc ggacgcatgg
      541 cctcgcggct ggaggccgac ggcggccgtc tggcccagat gcccgcccag tccaagaatc
      601 cgctggtgat gttgggcaat gccgcccggc tgatcgatct gatccggcgg gagaaggtca
      661 gtctggttca cgcccgttcc cgcgccccgg ccttcagcgc cctgtgggcg gcgcacgcca
      721 cgggcacgcc attcgtcgcc acctatcacg gcgtctacaa cgccaggtcc aatctgaagc
      781 gctggtacaa tgcggtgatg accaagggcg acctggtgat cgccaattcc gagtacaccc
      841 gcgctcacgt gatcaaggaa catgggatcg accccgcccg cgtcgtgtcc attccgcgcg
      901 gcgtcgacct gacccgtttc gagcccggca tggtcggcgg cgatcgcatc gcggcccttc
      961 gccaggcctg gggcgtcgcg cccgacgacc gtcggctgaa ggtgttgctg gcgggtcgcc
     1021 tgacccgctg gaagggccag gccctgctga tcgaggcgat ggccaatctg aaggccaggg
     1081 gcgaggcccg tgtgctgctg ctgctggtcg gcgatgatca gggccgcaag gcctatcgcg
     1141 ccgagctgga agcccagatc gccgctgccg acctgcagga cgacgtcagg atcgtcggcc
     1201 attgcgacga catgcctgcg gcctatcttc tggccgatct ggccatcgcc ccttcgatcg
     1261 agccggaagc cttcgggcgc acggcggtgg agccgcaggt tatgggacgt ccggtgctgg
     1321 cgtccgacca tggcgcggcg cgcgagaccg tgctgcctgg ccaaaccggc tggctggtcg
     1381 cgccgggcga cgcccaggcc tgggccgacg ccctcgccac ggcccgcgac gcaggcgccg
     1441 cgacccgcca gaccatgggc cagaccgccc gggcccgcgc tcggcggttg tattcagtag
     1501 acgccatgtg cgaagccacg ctgcaagtgt atgcgcgcgt gctggaagcc agagttttgg
     1561 agcggagacc gtgagtaagg aaatcaagaa ggtcctggtg atcaagctgg gggcccttgg
     1621 cgatttcgtt ctcgctctgg cggcgatgaa gaagatccgc gaggcccacc ccaaggccaa
     1681 gatcacgctt ctgaccacgc cccccttcga ggccctggcc aagctcagcc cctacttcaa
     1741 ttcggtcgag actgacgggc gcccggaaga tttcggcgac ctgacccaga tgctgggccg
     1801 gctgcgcaag gcccgctacg accgcgtcta cgacctgcag accaacagcc gcaccaactg
     1861 gtacttccag gccctgcggc cgttcccgcc gcaatggagc ggaatcgcct ttggctgttc
     1921 cctgccccag cgcaacaagg cccggcagac catgcacacg ctggaacgtc aggctgacca
     1981 gttgcgcgcc gccgggatct ggcccgacgc gccggtcgag catggcaacg ccccgccgcc
     2041 ggacctgtcc tggatcctgc gtcgccacaa ggaagcccgc cccgtcgccg gcgccgcagc
     2101 cccgcgcccc tatgtgctgt tcgtgccggg cggctcggcc caccggcccg aaaagcgctg
     2161 gccggtcgag gccttcgccc agctgggctc gctgctgaag gcccggggcc tggacatcgt
     2221 gatcatcggc ggaccgcagg aaagcgccat ggcgcgcgcc atccagaagt ccgtgggtca
     2281 ggcccgcgat ctgacgggcc gaaccgattt tgcgcaactg gccgtgctgg gcgccaaggc
     2341 ggcgctggtc gtcggcaatg acaccggtcc aacccatctg ctggccgccg ccggcgcccc
     2401 gaccatcgcc ctgttctctg acgcttcgga tcccgacctg tgcggcccgc gcggccacgt
     2461 ggcggtcatc cgatcccccg acctgaaggc cctgcccgtc tccaccgtcg ccagcgcagc
     2521 gatctcgctg ctgccctgac acaacgcgct agcggggctg agcacagcct tgggacaggc
     2581 cgttcttcgc cttgcgtcca aaaggtttgg cggcgctggg gtctgtttgg ctcgcgccgc
     2641 ttgatagggg gcgcccaaca ggtcatatat cgtttcctcg cagcgcgtct ccgcgcagcg
     2701 cgattacctt caactgtacc ggagccgccc ctattcgccg tcctatgcaa acgccgcccg
     2761 tcaaagacgg accgcgtatc aacgatgaaa tccgcgtccc gcgcgtcctg ctgatcgacc
     2821 aaaacggcga gaagcaaggc gagatgccga ccgcctccgc ccttgaagcg gccgaggaag
     2881 ctggtctgga cctggtggaa atcgttccaa acgccaatcc tcccgtctgc aagatcctgg
     2941 actacggcaa gttcaagttc caggagcaga agaagaagaa cgaggcgcgc aagcggcaga
     3001 agatcgtcga gctcaaggaa atcaagctcc gcccgaacat cgacatccac gactacgaag
     3061 tgaagaccaa ggccatgacc cggttcttcg aggagggcga caaggtcaag gtgaccctgc
     3121 gcttccgcgg ccgtgaaatg gcgcacccgg aactgggcat gaagctgctg atcaaggtga
     3181 agaccgactt tgaggaaatc gccaaggtcg agtacgagcc gcgcatggaa ggccggcaga
     3241 tgatcatgat cctggcgccg cgctaagcgc gtcaatccga tgtcgagaca gacgccccgg
     3301 ccctggccgg ggcgtttttc gtttgcggca agcgcgccag gacgtcgcgg tcacgacatt
     3361 cccgtcgcga gcctggccaa aaaagcgtat tcgggcggcc cttcgaaagc gccgcccatg
     3421 tccgagcccc tccccgccgc cgctcccgcc cacagcaacc gcgccctcta cgtcctgctg
     3481 ctggtggtgt tcatcaacct ggtgggcttc gggctggtga ttccgctgct gccgttctac
     3541 gcgcagtcgc tgaaggccga gccctggcag atcacggcgc tgttctcggc ctacagcctg
     3601 ggacagttct tcgccgagcc gttctggggc cggctgtcgg atcgcattgg ccgccgcccg
     3661 gtgctgatcg tgaccatcct ggccaatacc ctgtcctatg tggcgctggc cttcgccccg
     3721 accatctgga tcgccttcgc catccgcctg atcggcgggt tcggcagcgg taacatctcg
     3781 accatccagg gctacatggc cgacgtcacc ccgcccgagc agcgggccgc gcgcatggga
     3841 ctgctgggcg ccgcctttgg cgcgggtttc gtgatcggcc ccgccctggg cggcctgctg
     3901 gcccatcccg aagccggacg cctgggtttc cagatcccgc tgttcgtggc cgccggcctg
     3961 gcggccctgg cctcgctggg cgtgttcctg ttcgtggtcg agagccgctc gcccagcgcg
     4021 cccgacgccc tgcgcccgca tcgtcgcgaa gccctgcagg ccgccatggc ccatcccgtg
     4081 ctgtcgcggg tgctgctggt caccctggtc tcgaccgccg ccttcgccgg catggagtcg
     4141 gtctttggcc tgtggaccca tgcccggttc gactggggcc cgcgcgaggt tggcctgtgt
     4201 ttcgccgtca taggcgtcat cgcctcgatc ggtcagggcg cggtgaccgg ccgcctggcc
     4261 cggcgctttg gcgagcctcg cgttctgacg gcgggcctgt cgatcatcgc cttcagcctc
     4321 gccctgaccc cgttcgcccc cagcggagcg gccgtgcccc tgttcgtcgg cctgaccgcc
     4381 tttggccagt ccctggtctt tccctgcgtc gccgccctga tctcgcgcgc cacgccgcca
     4441 gaccagcagg gcgcgatgct gggtctcaac atggccgccg gctcattggc ccgcatggca
     4501 ggccccctgc tggccggccc gctgtttggc ctggcgctgg gtgggcccta ctggttcggc
     4561 gcccttctga ccctgccggc catctggctg ggggccagcc tggtccgccg cgccagggcc
     4621 ccggcctgat ccccggctcg acaacctgag cgagcgccac tatcgtctcg cttgtcgagt
     4681 tgaggggatt gcatgttgaa gatgttcgcg ggcgccgccc tggccgctct gatcggcatg
     4741 ggcgtggccc aggccgcgcc cctggaggcc tatggtcgac tgccggccat gagctcggtc
     4801 gatatctcca gtgatggcca gaaactggcc tatatccgca ccgacggcga gcgttcgacg
     4861 atcgtcgtgc agggcgtcga cggaaccgtg ctgcaggcgc tggaaatggg cgcgcgcaag
     4921 gtgcgcgacg tccagtggac cgacaacaac cacgtgctga tccgcatcac cgagaccacc
     4981 acggtcagcg gcatcttcta tgccggcgaa ttcgcgctgg gagccagcct caacgtcacg
     5041 acaggcaacg ccacctctct gcccagccgg accgccaccc gcgtcgccaa cgcgctgttc
     5101 ggcggccgcg ggggaaccta taatggcaag cccgtgctct atgccggcct gtttgcctcg
     5161 accgacggtc ccagcttcca gcataaggat ggcagcctgg acctctatcg gatcgatccc
     5221 gactcgggca tcggtcagtt gcaccagcgc ggcgactata cgaccggcca atacctcttc
     5281 aaatccgacg gcgaagtgct ggcgcgcacg caataccagt ccgaggacgg tcgctggtcc
     5341 ctggacctgc gccagaatgg ctggcgcgag gcctattccg tcatcgcgcc catcgacaca
     5401 cccgacctca tcggccgctc cctggacgaa acctcgatca ttctcaatgt ctgggatgac
     5461 aaggagcgca tagcgttcac tttttttctc caatcaggat atgcattaga agacacattg
     5521 gtg
//
LOCUS       DAOMEO010000042         5464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1926_length_5464_cov_2.519874, whole genome shotgun sequence.
ACCESSION   DAOMEO010000042 DAOMEO010000000
VERSION     DAOMEO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1926_length_5464_cov_2.519874"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLH31_01660"
     CDS             complement(<1..357)
                     /locus_tag="PLH31_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR87987.1"
                     /translation="MRAIHTTLLMSAASVLALSFASAGMAAEVETAQVDQVIVTAQKR
                     EQNRVEVPIAVTAYSGQFLEAIGVQEFDQLSAFTPGFVVQNQSNNNSGFVMRGITSDS
                     GSATDETRVSIYQDGVP"
     gene            538..1476
                     /locus_tag="PLH31_01665"
     CDS             538..1476
                     /locus_tag="PLH31_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQR87988.1"
                     /translation="MSANDAGERLDKVLATRFDSLSRARLQALIADGRLSLEGRAVTD
                     GSQKARPGLYQLLIPAPIPAEPSPEDIPLTVLYEDAHLIVVDKPPGMAAHPAPGNYTG
                     TLVHALLHHCRGSLSGVGGVARPGIVHRLDKETSGVMVAAKTDAAHQGLSALFSTHDI
                     DRLYIALTRGAPHPASGTIQTQLGRSPHDRKKMAILKSGGREAITHYRVEEAFGPADR
                     PLAARVTCRLETGRTHQIRVHLASKTAPCLGDPVYGGGPPAAGVKAVMEEIGFRRQAL
                     HAAVLGFVHPITGETLKFETPLPADMAALEAGLRAL"
     gene            1572..2264
                     /locus_tag="PLH31_01670"
     CDS             1572..2264
                     /locus_tag="PLH31_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR87989.1"
                     /translation="MTSTRSLIVLLMGLALAGPALAQGVTGQDEAVSTAAHRVDAKTA
                     AEAQAAPVAPTPAPRILTTDEQIAAFLAASPALERGEGLAGLAPTDEAARRKVHGSAG
                     VTVGTGGYRSAYVSALIPIGETSTLGLAYSQTDHGRNAVFFPRDAYGYGYGHDQGGWN
                     GDSVGLGRPGRWARGGKQQSVAVSLAVGSDRRGPPEGCAPAFRAGDRYVEPLWATQMR
                     GESSPCALDGTR"
     gene            2378..3223
                     /locus_tag="PLH31_01675"
     CDS             2378..3223
                     /locus_tag="PLH31_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR87990.1"
                     /translation="MQPEIIAHHHPATGTVTYLVVDPATLLCAIVDPVLDFDPETEAT
                     DTSFVDAIIAQIRARDMGPTWILETGLHAAHLSAAGHLKYETGASVCIGAGVIESLKR
                     LAPAMGEHGVDLDGWDFDKLAQDGEVMPMGGLEITAHAVTGRLPGAVAYQIGDAVFTG
                     DVILAPGLGAGRADLPGADAQALYAAARKFLSLPDDTRLMAGWEAAEGAATTTVAQQR
                     AQNVQLRDGVDLPTFVAARQAADAALPSLPYAAQALRVAIRSGKLPPQGDDGRRFPPT
                     DIPSF"
     gene            complement(3267..4535)
                     /locus_tag="PLH31_01680"
     CDS             complement(3267..4535)
                     /locus_tag="PLH31_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR87991.1"
                     /translation="MTRPFPIFGLLAALAATTTVSAGEAPGFARPSFLPADSAILSAL
                     DASPALAEARAGLQGARAQLRMSAAGDHEFMGSLTADRRQVRGEGDYAEWSAQISRGW
                     RSPGKRALDLKAGAAGERAAMDTVEDARHQASLQLASLWVGWLETEALADIDRREVET
                     YERDLLALTRRVELKDAAPLDRDQALAAAARAKSAATVSRAAAQSIRTELDTLYPQLA
                     PARPPELPAPLAPLRPFETWLTVIPQRSHEITIARALADREAALSRRARLDQRPDPTI
                     GLRAFNERGGAESGVGLAVSIPLGGALRSATAERQAATASAAEARYVMVVRSVDLTAK
                     RDVIEARSGLLAWEEALTSARAASVASSRIQRAYELGERDLTDLLAAKRQGFEAERQE
                     LRARSTAHAALLRLALDAHEYWLSEEGGAH"
     gene            complement(4532..>5082)
                     /locus_tag="PLH31_01685"
     CDS             complement(4532..>5082)
                     /locus_tag="PLH31_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR87992.1"
                     /translation="GGQFENQKRAAARLGVVVPASLALIFLVLFVTLRSIRQALLILA
                     NIPFAMIGGLVALWMSGEYLSVPAAVGLIALLGIAVLNGLVLVSHFNELLDRGLDLQR
                     AIVEGARRRLRPVLMTASITALGLVPLLLASGPGSEIQRPLAIVVVGGLITSTLLTLI
                     LLPILYRRFGLERGAALEESAS"
     assembly_gap    5083..5092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5093..>5464)
                     /locus_tag="PLH31_01690"
     CDS             complement(<5093..>5464)
                     /locus_tag="PLH31_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR87993.1"
                     /translation="SYLQFDVDRSAAVRAGLSVQAIQSELRAQLEGSSAGMVFEPGRR
                     TPIIVRGLENLRDDPGAFLQTQIATPDGGLVRAADVASVARREGPVKIDRENASRFAV
                     IQAYVSGRDLVGFVKEAQANVA"
BASE COUNT          920 a         1921 c         1816 g          797 t
ORIGIN      
        1 cggcacgccg tcctgataga tcgacacccg ggtctcgtcg gtggccgagc cgctgtcgga
       61 ggtgatgccg cgcatcacga agccggaatt gttgttggac tggttctgga ccacgaagcc
      121 cggcgtaaag gccgacagct ggtcgaactc ctgcacaccg atggcttcca ggaactggcc
      181 ggaataggcg gtgacggcga tcggcacctc gacgcggttc tgctcgcgct tctgggcggt
      241 gacgatgacc tggtcgacct gagcggtctc gacctcggcg gccatgccgg ccgaggcgaa
      301 gctcagggcc agaacgctgg cggctgacat gagcaaggtg gtgtggatcg cgcgcatcga
      361 ttatcccctt cgacgacaag ccggacttga gcccggcgga tagggaccgg cgatgtcggt
      421 cgcgcgacga gattgtgacg gaggcgtgaa acgcccttga acccgctgga cgacgataca
      481 gacgagcttg agaccgaggc gcaggcgcca caatccgagg ccaggcttga ggttgcgctg
      541 tcggcgaatg acgctgggga acgcctggac aaggtcctgg cgacgcgatt cgacagcctg
      601 tcgcgcgccc gcctgcaggc ccttatcgcg gatggacggc tcagccttga gggccgggcg
      661 gtgaccgacg gctcgcagaa ggcccgcccc ggcctctatc agttgctgat ccccgccccg
      721 atccccgccg agccctcgcc cgaggacatc cccctgacgg tgctctacga ggacgcccac
      781 ctgatcgtgg tcgacaagcc gcccggcatg gccgctcacc cggcccccgg caactatacc
      841 ggcaccctgg tccacgccct gctgcaccac tgccggggca gcctgtcggg cgtcggcggc
      901 gtggcccggc ccggcatcgt ccaccggctg gacaaggaaa cctccggcgt catggtggcg
      961 gccaagaccg acgccgccca ccagggtctg tcagcgctgt tttccaccca cgacatcgac
     1021 cggctctata tcgccctgac gcgcggcgcg ccccatcccg ccagcgggac gatccagacc
     1081 cagctgggcc gctcgccaca tgaccgcaag aagatggcga tcctgaaaag cggcggccgc
     1141 gaggcgatca cccattaccg ggtcgaggaa gccttcggtc ccgccgacag gccgttggcc
     1201 gcgcgggtga cgtgccggct ggaaaccggc cgcactcacc agatccgcgt ccacctggcc
     1261 agcaagaccg cgccgtgcct gggagatccg gtctatggcg gcggtccgcc cgccgccggc
     1321 gtcaaggccg tgatggagga gatcggcttc aggcgacagg ccctgcacgc cgctgtcctg
     1381 ggcttcgtgc atccgatcac cggcgagacc ctgaagttcg agacccccct gcccgccgac
     1441 atggcggcgc tggaagccgg gctcagagcg ctgtgagccg acactctctt gcggtcgcgt
     1501 tacaatcgcg ccctaattcg gctgggacgt cggcctagat cttgagacaa cgccgctgcc
     1561 ggaaagctcc gatgacctcg acgcgatcgc ttatcgttct cctgatgggc ctggccctgg
     1621 cggggccagc cctggctcag ggcgtgacgg gccaggacga agccgtctcg accgccgccc
     1681 atcgcgtcga cgccaagacg gctgccgaag cccaggccgc gcccgtcgct cccacccccg
     1741 cgccgcgcat cctgaccacc gatgagcaga tcgcagcgtt cctcgcggcc tcccctgccc
     1801 tggagcgcgg cgaaggcctg gcgggtctgg ctccgaccga cgaggccgcc aggcgcaagg
     1861 tccacggctc ggccggcgtc accgtgggta cgggcggcta tcgcagcgcc tatgtctccg
     1921 ccctgatccc catcggcgag acctccaccc tgggcctggc ctattcccag accgatcatg
     1981 gccgcaatgc cgtcttcttc ccacgtgacg cctatggata tggctacggc catgaccagg
     2041 gcggctggaa cggcgacagt gtcggcctgg gccgaccagg acgctgggcc cgcggcggaa
     2101 agcagcagtc cgtagccgtt tccctggccg tcgggagcga ccgcagagga ccgccagagg
     2161 gctgcgcccc ggcctttcgc gccggagatc gttatgtaga gccgctttgg gccacgcaga
     2221 tgcgcggaga atccagtccc tgcgcgctgg acggaacacg ctaatccgcc actcgccgta
     2281 gacgtgtcga aacaatcttc gcgcgagacg atcggcgttc gctgatgctt aacagcttgc
     2341 ggtcatggtt gcgtatcggg atctatcggg cgttcgcatg cagccggaaa tcattgctca
     2401 ccaccacccc gccacgggga cggtgaccta tcttgtcgtc gatccggcca cgctgctttg
     2461 cgcgatcgtc gacccggttc tggatttcga tccggaaacc gaagccaccg acaccagctt
     2521 cgtcgatgcg atcatcgccc agatccgcgc ccgcgacatg ggcccgacct ggatcctgga
     2581 aaccggcttg cacgccgcgc atctatcggc ggccggacac ctcaaatatg aaaccggcgc
     2641 gtcggtctgc atcggggctg gcgtgatcga aagcctcaag cgcctggccc ccgccatggg
     2701 cgagcacggc gtcgaccttg acggctggga ctttgacaag ctggcccagg acggcgaggt
     2761 catgccgatg ggcgggctgg agatcacggc ccacgccgtc accggtcgtc tgccgggcgc
     2821 cgtggcctat cagatcggcg acgcggtctt tacgggcgac gtcatcctgg ctccaggact
     2881 gggcgccggc cgcgctgacc ttcccggcgc cgacgcccag gccctctacg ccgccgcccg
     2941 caagttcctg tccctgcccg acgacacccg gctgatggcg ggctgggaag cagccgaggg
     3001 cgccgcgacc acgacggtcg cccagcagcg tgcgcagaat gttcagcttc gcgacggcgt
     3061 ggacctgccc accttcgtcg ccgcccgcca ggccgccgac gcggccctgc cgtccctgcc
     3121 ctatgccgcc caggcgctgc gtgtggccat ccggtcggga aagctgccgc cgcaaggcga
     3181 cgatgggcga cgcttcccgc cgacggatat cccttccttc tagccgatcg atcgagcagc
     3241 cctgatgggg cccggcgaac cagggatcag tgcgcgccgc cctcttcgga aagccagtac
     3301 tcgtgggcgt ccagagccag ccgcagaagc gcagcgtgcg ctgttgagcg ggcccgcagc
     3361 tcctgccgct cggcctcgaa gccctggcgc tttgcagcca gaagatcggt caggtcccgt
     3421 tcgcccagtt cgtaggcgcg ctgaattcgg ctggaggcga cgctcgcggc gcgggcggac
     3481 gttagggctt cctcccaggc cagaagaccg gaacgcgcct cgatcacgtc acgcttcgcc
     3541 gtcaggtcga ctgacctgac caccatgacg tagcgagcct cggcagcaga ggccgtcgcg
     3601 gcctgtcgct cggccgtcgc cgagcgcaaa gcgccgccca acgggatcga gaccgccaga
     3661 ccaaccccgc tttcagcccc gccccgttcg ttgaaggccc taaggccgat ggtcgggtcc
     3721 ggccgttgat ccagcctcgc gcggcgcgac aatgcggcct cgcgatcggc cagggcccgg
     3781 gcgatcgtga tctcatggct ccgctgcggg atcacggtaa gccaggtttc gaaaggacgg
     3841 agcggcgcca agggcgcagg cagttcgggc ggacgagccg gggcaagctg cggatacagc
     3901 gtatccagtt ctgtccgaat ggactgggcg gccgcgcggc tcaccgtcgc cgctgatttc
     3961 gcacgtgcag ccgccgccag cgcctgatcc cggtccaggg gcgctgcgtc tttcaattca
     4021 acgcggcgcg tcagcgccaa gagatcgcgc tcataggtct caacctcccg gcggtcgata
     4081 tcagcgagcg cctcggtctc cagccatccg acccaaaggc tcgccagttg aagcgacgcc
     4141 tgatgacgcg cgtcctcgac agtgtccatc gccgcgcgct cgccagccgc ccccgccttc
     4201 agatccagcg cgcgcttgcc tggtgatcgc cagccacggc tgatctgggc ggaccactcc
     4261 gcatagtcgc cctccccccg gacctggcgg cggtcagcgg tcaacgaacc catgaattcg
     4321 tggtcgccgg ccgcagacat ccggagctgc gcccgggcgc cctgaaggcc ggcgcgcgct
     4381 tcggccaggg ccggagacgc atcgagcgcc gagaggatcg cgctgtccgc tggcaggaag
     4441 gacggccgcg caaagccggg agcctcaccc gccgacacgg tcgtcgtggc ggccagggcg
     4501 gccagaagtc cgaaaatcgg gaaagggcgg gtcacgacgc ggactcctcg agagcagcgc
     4561 cgcgttccag accaaaccgg cgatagagaa tggggagaag gatcaaggtc aggagcgtcg
     4621 aggtgatcag gccgcccaca acgacgatcg cgaggggccg ctggatttca gaccctggac
     4681 cgctggccag cagcaggggc accagcccca gcgcggtgat gctggcggtc atcagaacgg
     4741 gccggagacg gcgccgggcg ccttcgacaa tggcccgctg aagatccagc ccgcggtcga
     4801 gcagctcatt gaagtgcgag accagcacaa ggccgttcag gaccgcgatc cccagcagcg
     4861 cgatcaggcc cacggcggcc ggcaccgaca gatactctcc ggacatccag agggcgacga
     4921 ggccgccgat catggcgaag gggatattgg ccaggatcag cagggcctgg cggatcgacc
     4981 ggagggtcac gaacagcacc aggaagatca gggcgaggct ggcgggaacg acgacgccca
     5041 gccgcgctgc ggcccgcttc tggttttcga actgcccgcc ccnnnnnnnn nngcgacatt
     5101 ggcctgagct tccttgacga agcccacgag gtcccggccg gagacatagg cctgaatgac
     5161 cgcaaagcgc gaggcgttct cgcgatcgat cttcaccggc ccttcgcgcc gcgcgacgct
     5221 ggcgacatcg gcggcgcgca ccagaccacc atccggagta gcgatctggg tctggagaaa
     5281 ggcgcctgga tcatcgcgga ggttctcaag gccgcgcacg atgatcggcg tccgtcggcc
     5341 aggctcgaac accatgccgg cgctggagcc ctcaagttgg gctcgcagtt ccgactggat
     5401 cgcctggacg gaaagacccg ccctgacggc agcgctgcga tcgacatcga actgcaggta
     5461 ggag
//
LOCUS       DAOMEO010000043         5414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1946_length_5414_cov_2.077067, whole genome shotgun sequence.
ACCESSION   DAOMEO010000043 DAOMEO010000000
VERSION     DAOMEO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_5414_cov_2.077067"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="hflK"
                     /locus_tag="PLH31_01695"
     CDS             complement(<1..854)
                     /gene="hflK"
                     /locus_tag="PLH31_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQR87994.1"
                     /translation="MPWNDNAGPGPWGSPPPSGDKDNTPRPGPRRPPGGPQAPIDVNQ
                     LMEDLTDRVRRYFQGPDRPRAIATAAGAVVALWAATGIYVVQPNQQGVVSTFGAFSRV
                     AGPGLRYHVPFPVESVEKVAVTEIRTTDIGGSGTEAGEERLMLTSDENIIDLSFSVQW
                     RVSDAPKYLFNVREPESVIRAVGESAMREVVGKTALTPILTTGRGQLQAETERLMQQI
                     LDRYDAGVFVESVQIQTATPPPPVIDAFRDVQRASQNAQSAANVARGQSAQIVQQAKG
                     YREQVVREA"
     gene            959..>1706
                     /locus_tag="PLH31_01700"
     CDS             959..>1706
                     /locus_tag="PLH31_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQR87995.1"
                     /translation="MTGAASTTLEDARAALDRVVDPVTGQGLVAAGLVQGLVVRNGRA
                     GFMLEVPAAKAAAYGTVRAAAEKALADLPGVDQAQVVLTAQAAEGATRVRKGARVSED
                     PQARMVPPPEAEKPAHVKHVIAVASGKGGVGKSTVATNLACAFAGMGLRVGLLDADIY
                     GPSAPRMMGVDGEPSFEDGKLQPLEAHGIKIMSIGFLVDEGKAMIWRGPMASSAVRQM
                     IHDVAWGSEAQPLDMLVVDLPPGTGDVQLTL"
     assembly_gap    1707..1716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1717..2054
                     /locus_tag="PLH31_01705"
                     /pseudo
     CDS             1717..2054
                     /locus_tag="PLH31_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
     gene            complement(2051..2542)
                     /locus_tag="PLH31_01710"
     CDS             complement(2051..2542)
                     /locus_tag="PLH31_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR87996.1"
                     /translation="MRIEKLRRDHAIDAFDCGGPALNRFLQKHALQSQQSGASQTYLA
                     LESESVVGFHSLVVGQIDYADAAERLTKGLARHPVPVMILARLAIAATHQGKGLGAGL
                     LKDAMARTLAAADIAGIRAFIVHAKDERARRFYERFDFLPSPTDPNHLFALMKDVRAA
                     LGN"
     gene            complement(2551..2838)
                     /locus_tag="PLH31_01715"
     CDS             complement(2551..2838)
                     /locus_tag="PLH31_01715"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HQR87997.1"
                     /translation="MAATAKSEKLDLRLTAEAKQMLQTAAAASHRSVSEFVLESAMAR
                     AQETLPDRHVFNLDAESWAAFQAALDAPPRSMPRLARLLSTPSVFERSDTE"
     gene            complement(2965..4263)
                     /locus_tag="PLH31_01720"
     CDS             complement(2965..4263)
                     /locus_tag="PLH31_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR87998.1"
                     /translation="MSFSRNRLAAGVAFGVLVAASQASASAFYLQEQSVRGAGRAYSG
                     EVADRGAASLWWNPASIAGLESNEVYGGLHAIQVDSDVNDSGSTITRPVPPAGLTTPV
                     GGDLRTSKPIKDGVVPNFASAWRINDQLTLGLSVAAPYNFTTEYGPRSWTRYDALKSR
                     LTTADLQGTVAYRVTDKLDLGVGVSAVYADAELTNALPNISPLQPDGYQSLKGDGWAY
                     GWTVGAQYRPTPALTLGASYRAKVEQTLKGDIAVSGLQAPIPAASNFKTAGEAEITTP
                     WMAIIGARWAINDRVTLNGSVSRIGWSEFDAIRVKYASGGSVSDQDYKDITTAAVGVD
                     YQASPRLTLRAGVQYDPTPTPDIGRTARVPDGDRMMYAAGATWAAKPNLKLDAAISYI
                     GFDDTKISRTDLTATSSTVNLRGDVTGSAVVMSTGVRYSF"
     gene            complement(4496..5401)
                     /locus_tag="PLH31_01725"
     CDS             complement(4496..5401)
                     /locus_tag="PLH31_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR87999.1"
                     /translation="MSDIRFDGKVAIVTGAGGGLGRQHALELARRGAKVVVNDLGGSV
                     DGSGGSSDAALKVVEEIKAAGGEAIAHGASVTDDAGVADLVKTTMDTWGRIDILIANA
                     GILRDKTLSKMELADFEAVMQVHVWGTFKPIKAVWDIMKAQNYGRIVVTTSSSGMYGN
                     FGQSNYGAAKMAVLGLMNTLKLEGQKNNVHINAISPVAATRMTEGLMPPEVLAKLAPE
                     AVTPGVMYLVSDEAPTGAILTAGAGAFALTRLYETEGVYLSDGELSAEGIRDHWGQIT
                     AEDGQKAYFNGGEQGQKFFRKMAGG"
BASE COUNT          852 a         1692 c         1887 g          973 t
ORIGIN      
        1 gcttcccgca cgacctgttc gcgatagccc ttggcctgct gcacgatctg ggcgctctgg
       61 ccgcgggcga cgttggcggc cgactgggcg ttctggctgg cgcgctgcac gtcgcggaag
      121 gcgtcgatga ccggcggcgg cggggttgcg gtctggatct gcacgctttc gacgaatacg
      181 ccggcgtcgt agcgatccag aatctgctgc atcagccgct cggtctcggc ctggagctgg
      241 ccgcggccgg tggtcaggat cggggtgagg gcggtcttgc cgaccacctc gcgcatggcg
      301 ctctcgccga cggcgcggat cacgctttcg ggttcgcgca cattgaacag gtacttgggg
      361 gcgtcggaga cccgccattg caccgagaag ctgaggtcga tgatgttctc gtcgctggtc
      421 agcatcaggc gttcttcgcc cgcctcggtt cctgagccgc caatatcggt ggtgcggatt
      481 tcagtgacgg ccaccttctc gaccgactcc accgggaacg gcacatgata gcgcaggcct
      541 gggccggcga cgcgcgagaa cgcgccaaag gtgctgacca cgccctgctg gttgggctga
      601 accacataga tgccggtcgc ggcccacaga gccacgacgg cgccggcggc ggtggcgatc
      661 gcgcgagggc ggtcagggcc ctggaagtag cgacgcacgc ggtcggtcag gtcttccatc
      721 aactggttta catcgatcgg cgcctgcggc ccgccgggcg ggcggcgcgg accggggcga
      781 ggggtattgt ccttgtcgcc gctgggcggc ggagaacccc agggaccagg gccagcattg
      841 tcgttccacg gcatgagcgg cgtcgtcttc ccgtcgtctt ttgattttca gcggggattg
      901 taaatcgtgc cccacacgcg gatatgagga cccctgctca acaaggcaag acattgacgt
      961 gaccggcgct gcttccacaa ccctcgaaga tgctcgcgcg gctctggatc gggtcgttga
     1021 tccggtcacc gggcaggggc ttgtcgccgc cggattggtg cagggtctgg tggtgcgcaa
     1081 cggccgcgcc ggcttcatgc tggaagttcc cgccgccaag gccgccgcct atgggaccgt
     1141 gcgcgccgcc gccgaaaagg cgctggcgga cctgccgggc gtcgatcagg cccaggtggt
     1201 cctgactgcc caggcggccg aaggcgcgac ccgggtgcgc aagggcgccc gggtttcaga
     1261 agacccgcag gcccgcatgg tcccgccgcc ggaagccgag aagccggctc atgtgaagca
     1321 cgtgatcgcc gtcgcctcgg gcaagggcgg ggtcggcaag tccaccgtcg ccaccaacct
     1381 ggcctgcgcc ttcgccggca tgggcctgcg cgtggggctg ctggacgccg acatctacgg
     1441 cccctcggcc ccccgcatga tgggcgtcga cggcgaaccc agcttcgagg acggcaagct
     1501 gcagcctctg gaggctcacg gcatcaagat catgtcgatc ggcttcctgg tcgacgaggg
     1561 caaggcaatg atctggcgtg ggccgatggc ctcgtcggcg gtgcgccaga tgatccacga
     1621 tgtggcctgg ggctcagagg cgcagccgct ggacatgctg gtcgtcgatc tgccgcctgg
     1681 caccggcgac gtgcaactga ccctggnnnn nnnnnntgag agcggacgaa ttattgctgt
     1741 ggaggcattg atacggtggt tgcatcctga tcatgtttgg caagaccgcg accccgatcc
     1801 tgggcctgat cgagaacatg gccttcttcg ccgatcccgc gaccggcgcc ccgatcccga
     1861 tcttcggttc cggcggcggc gtggccgagg ccaggacgct gggcgtgccg gtgctggccg
     1921 aggttccgat cgaactggcc gtccgcgaag gcggcgatgg cggggttccg gtcgtgctgg
     1981 gcaagcccga cagcgcggcg gccaaggcct ttgcgggggc ggctgaaatg ctgtggggcg
     2041 tggtgaaggg ctagttgccc aacgcggccc gcacgtcttt catcaatgcg aataaatggt
     2101 tcggatcggt gggcgaaggc aggaagtcga accgttcgta aaagcgccgg gcgcgctcgt
     2161 ccttggcatg aacgatgaag gcccggatgc ccgctatatc cgctgccgcg agcgtccgcg
     2221 ccatggcgtc tttcagcagg cccgcgccca gccccttgcc ctgatgagtg gcggcgatgg
     2281 ccaggcgcgc caggatcatc accggaacgg gatggcgcgc caggcccttg gtcaggcgct
     2341 cggcggcgtc ggcatagtcg atctggccaa cgacgaggct gtgaaaaccg acgacacttt
     2401 cgctctcaag cgccagatag gtttgagacg cgcccgattg ctggctctgc agagcgtgtt
     2461 tctgcaaaaa gcgattcagg gccggcccgc cgcagtcgaa agcgtcgatg gcgtgatcgc
     2521 gtcgcagttt ttcgatccgc acagcttacg tcattcagtg tcggagcgct cgaacacgct
     2581 gggcgtcgac agcaggcggg cgaggcgagg catggaccgg ggcggcgcat ccagagcggc
     2641 ttgaaaggcc gcccagcttt ctgcgtcgag attgaagacg tgacggtcgg gaagggtttc
     2701 ctgggcgcgc gccatggcgc tttccagcac gaattcactg actgagcggt ggctcgcggc
     2761 ggcggctgtt tgcagcatct gctttgcttc cgctgtcagg cgtaggtcga gcttctctga
     2821 tttcgctgtt gcggccatgg tttctcgcct cgatgtggca gaatggcata tttgcccgac
     2881 aatgtcatga cactagtcgt cgccacaaaa agggccgccc gagcatcgcc cggacggcct
     2941 cttccgttca gcctatgcca gcctctagaa gctgtagcga acgccggtcg acatcacgac
     3001 ggccgacccc gtcacatcgc cgcgcaggtt gacggtcgaa ctggtcgccg tcaggtcggt
     3061 gcggctgatc ttggtgtcgt caaagccgat gtagctgatc gccgcgtcga gcttcaggtt
     3121 cggcttggcc gcccaggtgg cgccggccgc gtacatcatg cggtcgccgt ccggcacgcg
     3181 ggccgtgcgg ccgatgtccg gggtcggggt cgggtcgtac tgaacgccgg cccgcagggt
     3241 caggcgcggg ctggcctgat aatcgacgcc gacggcggcc gtggtgatgt ccttgtagtc
     3301 ctgatcgctg accgagccgc cggaggcgta cttgacgcga atggcgtcga actcgctcca
     3361 gccgatgcgg ctgaccgagc cgttcagggt gacgcggtca ttgatcgccc agcgcgcgcc
     3421 gatgatggcc atccacgggg tggtgatctc ggcttcgccg gcggtcttga agttgctggc
     3481 ggccgggatc ggggcctgca ggcccgaaac ggcgatgtcg cccttcaggg tctgctcgac
     3541 cttggcgcga tagctggcgc ccagggtcag ggccggggtg gggcggtatt gcgcgcccac
     3601 cgtccagcca taggcccagc cgtcgccctt cagcgactga tagccgtcgg gctgcaaggg
     3661 cgagatgttg ggcagggcgt tggtcagttc ggcgtcggca tagacggcgc tgacgcccac
     3721 gccgaggtcc agcttgtcgg tgacgcgata ggccaccgtg ccctgaaggt cggcggtggt
     3781 caggcgcgac ttcagcgcgt catagcgggt ccagctgcgc ggaccgtatt cggtggtgaa
     3841 attgtagggc gcggcgaccg acaggcccag ggtcagttgg tcgttgatcc gccaggcgct
     3901 ggcgaagttg gggaccacgc cgtccttgat cggcttggag gtgcgcaggt cgccgccgac
     3961 cggggtggtc aggccagcag gcggcaccgg gcgggtgatg gtcgagccgc tgtcattgac
     4021 gtcggaatcg acctggatgg cgtgcaggcc gccatagact tcgttgcttt ccaggccagc
     4081 gatcgaggcg gggttccacc acaggctggc ggcgccgcga tcagcaacct cgcccgaata
     4141 ggcccggccc gcgccgcgca ccgattgttc ctggagatag aaggccgaag ccgaggcctg
     4201 ggaggcggcg acgagcacgc cgaaagccac gccggcggcg aggcggttac gcgaaaaaga
     4261 catgagactg acgagacctt cttggggagg agaagtcccc gagcgtcttt cggcgccaga
     4321 ggagcgccgc gtctaacgga atgagccaga aaccgttgct atttttgtgt cacacggtga
     4381 attttataca ggtagtcaaa gaaacggggc catgaaggcc ccgtttcaat cactgttatg
     4441 tcagtaatgg ctggaatgtg acgctactgg tgtcactttc actgcaagac tattattagc
     4501 cgcccgccat cttgcggaag aacttctgac cctgctcgcc gccattgaaa taggccttct
     4561 ggccgtcttc ggcggtgatc tggccccagt ggtcgcggat gccctcggct gacagttcgc
     4621 cgtcgctgag atagaccccc tcggtctcat agaggcgcgt cagggcgaag gcgccggcgc
     4681 cggcggtcag gatcgcgccg gtcggggcct cgtcagagac cagatacatc acgccgggcg
     4741 tcacggcctc gggggccagc ttggccagca cttcgggcgg catcaggcct tcggtcatgc
     4801 gggtggcggc caccgggctg atggcgttga tgtggacgtt gttcttctgg ccttcgagct
     4861 tgagggtgtt cattaggccc agcaccgcca tcttggccgc gccatagttg gactggccga
     4921 aattgccata catgcccgac gacgaggtcg tcacgacgat ccggccatag ttctgggcct
     4981 tcatgatgtc ccagacagcc ttgatcggct tgaaggtgcc ccagacgtgg acctgcatga
     5041 ccgcctcgaa gtcggccagc tccatcttgc tcagggtctt gtcgcgcagg atgccggcat
     5101 tggcgatcag gatgtcgatg cggccccagg tgtccatggt ggtcttgacc aggtcggcga
     5161 cgccagcgtc gtcggtcacc gaggcgccgt gggcgatggc ttcgccgccc gccgccttga
     5221 tctcttccac gaccttcagg gccgcgtcgg aagagccgcc cgagccatcg acgctgccgc
     5281 ccaggtcgtt gaccaccacc ttggcgccgc gacgggccag ttccagagcg tgctgtcggc
     5341 ccaggccgcc gcctgcgccc gtaacgatcg ccaccttgcc gtcaaaacgg atgtccgaca
     5401 tgccctagtc tccc
//
LOCUS       DAOMEO010000044         5412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1949_length_5412_cov_2.748740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000044 DAOMEO010000000
VERSION     DAOMEO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_5412_cov_2.748740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..536)
                     /locus_tag="PLH31_01730"
                     /pseudo
     CDS             complement(1..536)
                     /locus_tag="PLH31_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            728..>1021
                     /locus_tag="PLH31_01735"
     CDS             728..>1021
                     /locus_tag="PLH31_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518426.1"
                     /note="seems to be involved in biofilm formation; in
                     asymptomatic bacteriuria E. coli strains 83972 and VR50,
                     the ygiD gene is upregulated during biofilm formation in
                     urine; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HQR88000.1"
                     /translation="MTRMPALFVGHGSPMNAIEDNAWSRAWAAIGRDLPRPRAIAMVS
                     AHWETRGATAVSAAPHPETIHDFGGFPQTLFDVRYPAPGHPALAARIGEQLSPD"
     assembly_gap    1022..1046
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1047..1502
                     /locus_tag="PLH31_01740"
     CDS             <1047..1502
                     /locus_tag="PLH31_01740"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQR88001.1"
                     /translation="RGLDHGAWGVLKPMYPDADIPVVQISLDRDRSSRWHYEAGRRLS
                     ALRDEGVLMMGSGDIVHNLRAADFRRPEAPEWADRFNETAKALILAGDHDPLIDWETL
                     GPDAEIAINSAEHYLPLLYVLGAQGAGEPVSFFTDDVFAAISMTGVKVG"
     gene            1743..>2178
                     /locus_tag="PLH31_01745"
     CDS             1743..>2178
                     /locus_tag="PLH31_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HQR88002.1"
                     /translation="MTKPLKIDFVSDVSCPWCIVGLGGLETALERVGDLVEAEIHFQP
                     FELNPNMPPEGQNIVEHIGEKYGATPEQSAANRKAIHERAASVGFTMKTSETSRIYNT
                     FDAHRLLHWAGIIGGQKALKRALFDAYFTDGLSPADHDVLVAT"
     assembly_gap    2179..2188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2189..2401
                     /locus_tag="PLH31_01750"
     CDS             <2189..2401
                     /locus_tag="PLH31_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR88003.1"
                     /translation="AQKAGLDGAAARDVLASGQYAQEVREAERAWHTAGISSVPAVII
                     NDRYLISGGQPAEYFEKALRQIAAEA"
     gene            complement(2406..4100)
                     /locus_tag="PLH31_01755"
     CDS             complement(2406..4100)
                     /locus_tag="PLH31_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR88004.1"
                     /translation="MRKPWLLLVLGLVLVLGGSALAWKVQTAGGIKVTEVSFPGTGGA
                     PMSGLLYTPPNATPATPAPGVLAVHGYINSRETQSGFAIEFARRGYVVLALDQTGHGY
                     SGGAAFSNGFGGPDGLNYLRGLPTVDPANIGLEGHSMGGWTVLAAAATMPDAYKAVVL
                     EGSSTGKPFAAEGSPTWPRNLALVFSQYDEFSKLMWGVDRARDVTGSPKLQTVFGATA
                     PVQPGRVYGSIADGTARVLQTPATTHPGDHISPTAIGHALDWFAATLTGGTPRPAGDQ
                     IWMWKEAGTGLALVGAVALLLGTFQVLLTLPLFAALRQPIQPTVARRDRGWWSAVALT
                     ALLPAVTYFPFFILGMVALPPNAIFKQGITNQVLTWAALTALITLGLALLGKRDKPLF
                     SNRWRLSLLIGLFSVAVVYGALELVHRLFLVDFRFWVVALKRLSPGQADLVLVYVLPL
                     TLAFGVTLRTLLARIPVAGERNLARYAGAVLALSGGFALLLALDYGALFLTGRLPTAF
                     DPLSTVVAIQFLPLTALIAVIGVFTWKRTGGYAVATVICGLLLTWYMVAGTATQVV"
     gene            complement(4384..5070)
                     /locus_tag="PLH31_01760"
     CDS             complement(4384..5070)
                     /locus_tag="PLH31_01760"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQR88005.1"
                     /translation="MAEPQPLNIVFALFPGVTHLDFTGPHQILCRLPGAKVTVASLEG
                     GEIEADGLIFAHLPRLTDVEACDVLVVPGGFGTTEAMANPAFLDEIRRLAAGARYVTS
                     VCTGSLVLGAAGLLKGKRAACHWAWRDQLSLFGAIPDPGRVARDGNVFTGGGVTAGID
                     FALTIAAEIAGEDVAQTIQLAVEYAPAPPFNAGRPETAPARVLERVQRLYGQGMPERL
                     AAAEKAGALV"
     gene            5149..>5412
                     /locus_tag="PLH31_01765"
     CDS             5149..>5412
                     /locus_tag="PLH31_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HQR88006.1"
                     /translation="MPRTIGFLVYPDFQLLDATGPIATFEIAARFSPGAYRLVFLSKD
                     GGMVASSSGLTMATTALADAPPLDTLILAGGDGVDAGGPAGLVL"
BASE COUNT          943 a         1868 c         1822 g          744 t
ORIGIN      
        1 tcgaagacca gcaccacggc tgcgccgacc agcggcagca ccaccgcgtg gcgcacccgg
       61 tcggccgcct tccacaggaa cgggaagatc gcgtagcaga cgatcagggc agacagcgac
      121 cagctgggca ggttccagcc gtcgctgggg aagccccagg cgtggaccag cagggcctgg
      181 accggcagct ggtcccaggc aaagcggctg ggcttgtggg cgtcggtgcc gatggcgttc
      241 aggatcagca ccagcacggc catggccgcc agcaccatca ggtgccccgg ccagacccgc
      301 gcggcgcggc gaacccagaa ccggcccggc gtgatgtgac ctgacagggt cgaggcgcca
      361 taggcccggc ccagcacata gcccgacagg atcaggaaga agtccgtggc cagaaagccg
      421 cgcgaaaaca cctggtgaaa gcgctcgatc cgcagcgggc tctcggcccc gaagtggtag
      481 agcacgatca gcagcgaggc caggaaccgc aggaagtcca gggccccgcc ccgaacggcg
      541 cggcgggcgc gggccattac cggcgtctcg accgtggcgg aactgtcagg gggcgaagca
      601 gagctggaca ccgccatggc cttaacaaag cccgccgcgc cggaaaagcc gtcgcgtggg
      661 ttccgcttgc ggtatggcga cgcggcgcct atctgcaacc agtactgttt ctaaaaaagg
      721 cgccgccatg acccgcatgc ccgccctgtt tgtcggccac ggctcgccga tgaacgccat
      781 cgaagacaac gcctggagtc gggcctgggc cgcgataggc cgcgacctgc cgcgcccgcg
      841 cgccatcgcc atggtcagcg cgcactggga gacgcgcggc gccaccgccg tcagcgccgc
      901 gccgcacccc gagaccatcc atgatttcgg cggcttcccg cagacgctgt tcgacgtgcg
      961 atacccggcg ccgggccatc cggccctggc ggcgaggatt ggcgagcagc tgtcgcccga
     1021 tnnnnnnnnn nnnnnnnnnn nnnnnncggg gcctcgacca cggcgcctgg ggcgtgctga
     1081 agccgatgta tcccgacgcc gacattcccg tggtgcagat cagcctcgac cgcgaccgct
     1141 ccagccgctg gcactacgag gccggtcgcc gcctctcggc cctgcgcgac gagggcgtgc
     1201 tgatgatggg cagcggcgac atcgtccaca acctgcgcgc cgccgacttt cgccggcccg
     1261 aggcgccaga atgggccgac cgcttcaacg agactgccaa ggcgcttatc ctggcggggg
     1321 accatgatcc gctgatcgac tgggagaccc tcggccccga cgccgagatc gcgatcaaca
     1381 gcgccgagca ctatctgccg ctcctctacg tgctgggcgc gcagggggcg ggcgagccgg
     1441 tcagcttctt caccgacgac gtcttcgcgg cgatctcgat gacgggggtc aaggtcggct
     1501 gaatacgaac aacgcccgtg ccgggggagc acgagcgttg cgcgtttaag gacgccgggg
     1561 ccggccgggg ggaagccggg ttgtccgcgt cgagtcctag attggtgggc gaacccggct
     1621 ttgcaacggc gcgactgggg tcatttcaag accgactgtg gcaggcggca aggtcagcgc
     1681 cgggctggaa ccgtccgcgc ccgcgcctat atctgcaagc gcaacacgcc aggacctcgc
     1741 ccatgaccaa gcccctgaag atcgatttcg tctccgacgt ctcctgcccc tggtgcatcg
     1801 tcggcctggg cgggctggag acggccttgg agcgtgtggg ggatctggtc gaggcggaga
     1861 tccatttcca gcccttcgag ctgaatccca acatgccgcc cgaaggtcag aacatcgtcg
     1921 agcatatcgg cgagaagtat ggcgcgacgc cggagcagtc ggccgccaac cgcaaggcga
     1981 ttcacgagcg tgcggcctcg gtgggcttta cgatgaagac cagcgagacc agccgcatct
     2041 acaacacctt cgacgcccac cgcctgctgc actgggccgg catcattggc ggccagaagg
     2101 ccctgaagcg cgcgctgttc gacgcctatt tcaccgacgg cctcagcccc gccgaccatg
     2161 atgtcctggt cgcgaccgnn nnnnnnnngc gcaaaaggcc ggcctggacg gcgcggcggc
     2221 ccgcgacgtg ctggcctccg gccagtacgc ccaagaagtg cgcgaagccg agcgcgcctg
     2281 gcataccgcg ggcatcagct cggtccccgc cgtgatcatc aatgaccgct acctgatctc
     2341 gggcggccag ccggcggagt atttcgagaa ggccctccgc cagatcgccg ccgaggcctg
     2401 agccctcaca ccacctgcgt cgccgttccc gccaccatgt accaggtcag cagcaggccg
     2461 cagatcacgg tcgcgaccgc atagccgccg gtgcgcttcc aggtgaagac gccgatcacc
     2521 gcgatcaggg ccgtcagggg caggaactgg atggccacca cggtcgacag cgggtcgaac
     2581 gccgtgggca gccggccggt caggaacagc gcgccgtagt cgagcgccag cagcagggcg
     2641 aagccgccgc tgagcgccag gacggcgccg gcatagcggg ccaggtttct ctcgcccgcc
     2701 accgggatgc gggccagcag ggtgcgcagg gtgaccccga aggccagggt gaggggcagc
     2761 acatagacca gcaccagatc ggcctggccg gggctgaggc gcttcagggc cacgacccag
     2821 aagcggaaat cgaccaggaa caggcgatgg accagctcca gcgcgccata gaccaccgcc
     2881 acgctgaaca gaccgatcag cagcgacagc cgccagcgat tgctgaacaa cggcttgtcg
     2941 cgcttgccca gaagcgcgag cccaagggtg atcagggccg tcagcgccgc ccaggtcagg
     3001 acctggttgg taatgccctg cttgaagatt gcgttaggcg gcagggccac catgcccagg
     3061 atgaagaacg ggaaataggt cacggccggc agcagggcgg tcagggcgac agcgctccac
     3121 cagccccggt cgcggcgggc gacggtcggc tgaatcggct gacgcagagc cgcgaacagc
     3181 ggcagggtca gcagcacctg gaaggtcccc agcagcagag ccaccgcccc gaccagggcg
     3241 agacccgtgc cggcctcctt ccacatccag atctggtcgc cggccgggcg cggggtcccg
     3301 cccgtcagag tggcggcgaa ccagtccagg gcgtggccga tggcggtcgg tgagatatgg
     3361 tcgccgggat gggtggtggc gggggtttgc agcacccgcg ccgtcccgtc ggcgatcgag
     3421 ccatagacgc ggcccggctg gaccggcgcc gtggctccaa acacggtctg cagcttggga
     3481 ctgcccgtga cgtcacgtgc ccggtcgact ccccacatca gcttggagaa ctcgtcgtac
     3541 tggctgaaca ccagggccag gttcctgggc caggtcggcg agccctcggc ggcgaacggc
     3601 ttgccggtcg aggagccttc cagcaccacg gccttgtagg cgtcaggcat ggtcgccgcg
     3661 gcggccagca ccgtccagcc gcccatgctg tggccttcca gcccgatatt ggccgggtcg
     3721 acggtcggca ggccgcgcag atagttcagg ccatcgggac cgccaaagcc gttcgagaac
     3781 gccgccccgc cgctatagcc gtggccagtc tggtcgagcg ccagaaccac atagccgcgc
     3841 cgcgcgaact cgatggcgaa gccgctctgg gtctcgcggg aattgatata gccgtgcacg
     3901 gccagcacgc cgggggccgg agtcgcgggc gtggcgttgg gcggcgtgta gagcaggccg
     3961 ctcatcggcg cgccgccggt tccggggaag ctgacctcgg ttaccttgat cccgcccgcc
     4021 gtctggacct tccaggccag cgcgcttccg cccagcacca ggacaagacc cagcaccagc
     4081 aacagccatg gtttgcgcat gacatcccct cccccacgcc ggtcgttcgc cggctttgaa
     4141 gagagggtag atgaggatgg tggaatgtcg aatccggctt ctatctccag ttcactatcc
     4201 aagaacccgc cttcctaggc cttgcaccta ggacccattg tcgagggcgg cgctgtcttg
     4261 aaatcctagc gatgcggaaa ggccaagagc accggccctc tcagctgttc ctgccggcat
     4321 ggatcctggg cacaaggccc aggaaggcaa cgaaattggg cgttaggtta ggatggagtg
     4381 cgcctaaacc agcgccccgg ccttctccgc cgccgccaga cgctcgggca tgccctggcc
     4441 gtagaggcgc tgcacccgct ccagcaccct ggccggcgcg gtctcgggcc gcccggcgtt
     4501 gaacggcggg gccggggcat attccacggc cagctggatc gtctgggcca cgtcctcgcc
     4561 ggcgatctcg gcggcgatgg tcagggcaaa atcgatgccg gccgtgaccc cgccgccggt
     4621 gaagacattg ccgtcgcgcg ccacacggcc gggatcaggg atcgcgccaa acagagaaag
     4681 ctgatcgcgc cacgcccagt gacaggccgc ccgcttgccc ttcaacagcc ccgcagcgcc
     4741 cagcaccagc gagccggtgc aaaccgacgt cacatagcgg gccccggcgg ccaggcggcg
     4801 gatctcatcg aggaaggccg gattggccat ggcctccgtc gtcccgaacc cgccggggac
     4861 caccagcaca tcgcacgctt cgacatcggt caggcgcggc aggtgcgcga agatcaggcc
     4921 gtcagcctcg atttcgccgc cctccaggct ggcgaccgtg actttcgcgc cgggaaggcg
     4981 gcacaggatc tggtgaggtc cggtaaagtc caggtgcgtc acgcccggaa acagggcgaa
     5041 gacgatattc agcggttgcg gctcggccat ggggacatct ccgtttgacg ccgcaccatc
     5101 ccgcgcctag cgtctggctg aaatgacata attccctcgg attccgccat gccccgcacg
     5161 atcggctttc tggtctatcc cgacttccag ctgctggacg ccacggggcc gatcgccacc
     5221 ttcgagatcg ccgcgcggtt ctcgccgggg gcctaccggc tggtctttct gtcgaaggac
     5281 ggcggcatgg tggccagctc gtctggcctg accatggcca ccaccgcact agctgacgcg
     5341 ccgccgctgg acaccctgat cctggcgggc ggcgacggcg tcgacgcggg cggcccagcg
     5401 ggcctcgtcc tc
//
LOCUS       DAOMEO010000045         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1951_length_5405_cov_2.916636, whole genome shotgun sequence.
ACCESSION   DAOMEO010000045 DAOMEO010000000
VERSION     DAOMEO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_5405_cov_2.916636"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..328)
                     /locus_tag="PLH31_01770"
     CDS             complement(47..328)
                     /locus_tag="PLH31_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR88007.1"
                     /translation="MIRGFADRETRRLWEGIRSRRLPPDIQDAALRKLRLLNRVVRLE
                     DLRLPPGNRLEALSGDRAGQHSIRINSQWRICFRWREESVEDVEICDYH"
     gene            complement(487..837)
                     /locus_tag="PLH31_01775"
     CDS             complement(487..837)
                     /locus_tag="PLH31_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HQR88008.1"
                     /translation="MLYLILKAALSGVIIAAISEIARKSPGFGALVASLPLISILGMI
                     WLWRDTRDITRMASHVEATFWYVLPSLPMFLVIPALLRRGLGFWPALLAGCVLTIGLY
                     WAMMAVGPRFGVKL"
     gene            942..1187
                     /locus_tag="PLH31_01780"
     CDS             942..1187
                     /locus_tag="PLH31_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HQR88009.1"
                     /translation="MTDITYTIAEHDGGWAYKLGDVFSETYPSHDAALAAAQRAAAEQ
                     QVAGETTGISYEDAKGQWREEIAAGDDRPTTHVVDSQ"
     gene            1261..1686
                     /locus_tag="PLH31_01785"
                     /pseudo
     CDS             1261..1686
                     /locus_tag="PLH31_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518490.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
     gene            complement(1992..2156)
                     /locus_tag="PLH31_01790"
                     /pseudo
     CDS             complement(1992..2156)
                     /locus_tag="PLH31_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2469..2894)
                     /locus_tag="PLH31_01795"
     CDS             complement(2469..2894)
                     /locus_tag="PLH31_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88010.1"
                     /translation="MARKISTTIGHVAAGLAALSMVAAATTASADSRRHHRHHRNDNK
                     GAEAAIVAGVIGLALGAAIASSSNGNDRYARPSRGYGNGYGAGSGYGYGNGYSNGYDY
                     APPPRYYGRPHNGGYREDCWTTREYDRRSGAVYERTVCR"
     gene            3092..3562
                     /locus_tag="PLH31_01800"
     CDS             3092..3562
                     /locus_tag="PLH31_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR88011.1"
                     /translation="MTSSVAYKTGEALQVLSFEVGTQTYCVPVNSVREIRGVTPPTQL
                     PDAPPYVRGVINLRGQVMPVIDLSARLGKGVAEDGPRQVIIVIESGDDVAGLLVDAVC
                     DSFIVDGDQINPPPSMGEENSPLVSAVITSDTGMTVLLSVGHIIPERAAPAIAA"
     gene            3659..4792
                     /locus_tag="PLH31_01805"
     CDS             3659..4792
                     /locus_tag="PLH31_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HQR88012.1"
                     /translation="MKAAVLREVGKPLQIETVSIGKPGPREVLIRTKAAGVCHSDLHF
                     VEGSYPHPLPAVLGHESAGIVEAVGSEVRTVKVGDHVITCLNPYCGHCEVCLTGHMNL
                     CISPETRRSKTDEPRLFKEDLSGSGDTKMAQFLNLSSFAEMMLVHEHACVAIRRDMPF
                     DLAALIGCSVMTGVGAVIHTSSVRPGETVAVIGCGGVGLATINGAAIAGAGRIIAIDR
                     VASKLELAKQFGATDLINADDGVCSGKEVDDDGDVVKQVQELTGGGVQHSFEAIGLKV
                     TAEQSFKMLRRGGTANIIGMIPVGTKIELTGSDFLGERRIQGSYMGSNRFPVDMPRLV
                     DFYMSGKLKLDQMISRRIKLEEINSAFDELKRGELARSVIVFD"
     gene            complement(4877..>5405)
                     /locus_tag="PLH31_01810"
     CDS             complement(4877..>5405)
                     /locus_tag="PLH31_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQR88013.1"
                     /translation="GVKMLFAGDKPLDLNTNPALKWLRRVMPVSDKLHGDRFFVQGVD
                     PRTGKVARLATPLFLALVLVELADLVFAVDSVPAIFAITTDPYIVYTSNIMAILGLRA
                     LYFALAAVLHRFAYLKQALAVLLVFIGGKIFIADALGWEKFPAALSLSITFAILAAGV
                     GWSLWKTRQVQPAKA"
BASE COUNT          998 a         1825 c         1734 g          848 t
ORIGIN      
        1 gtcgccgggc gtggggtttt ttagccagtc ctcgggcgag cccacatcag tgatagtcgc
       61 agatttcgac atcctcgacg ctttcctcac gccagcggaa gcagatccgc cactggctgt
      121 tgatgcgaat ggagtgctgc cccgcgcgat cgcccgacag cgcctccagt cggttgcccg
      181 gtgggagccg caagtcctcc agacgcacca cccggttcag aagacgcagc tttcggaggg
      241 ccgcgtcctg gatgtcgggc gggaggcggc gggatcgtat gccctcccac agacgccggg
      301 tctcgcggtc agcaaaaccc ctgatcacga aggtctatca taacgcataa cgttataagg
      361 gtcaatattc tgatacatgg tccgcacccg atatcgatcc atgcgatttt atcgggaaca
      421 attcacaaat atagtctcag attgtaaagc gggggctcgc ctgtctggcc gggaggcaaa
      481 aggtccctac agcttcaccc caaacctcgg ccccaccgcc atcatcgccc agtagagccc
      541 gatcgtcaga acgcagccgg ccagcagggc cggccagaag cccaggccgc gtcgcagcag
      601 cgccgggatg accaggaaca tcggcaggga cggcagcaca taccagaagg tggcctcgac
      661 atggctggcc atgcgggtga tgtcgcgggt gtcgcgccaa agccagatca tgcccaggat
      721 cgagatcagc ggcagcgaag ccaccagggc gccaaagccc ggtgacttgc gggcgatctc
      781 cgagatcgcg gcgatgatga cgcccgagag cgcggccttg aggatgagat acaacatccc
      841 gctgttgtcc gcgctccaac ccttcccgac aagacggggc cttcggaacc cgggctactg
      901 acggctcgtt gatccttcca cagcctcagg agccctcccc catgaccgac atcacctaca
      961 cgatcgccga gcacgacggc ggctgggcct acaagctggg cgacgtgttc tcggaaacct
     1021 atcccagcca tgacgcggcg ctagccgccg cccagcgggc cgcagccgag caacaggtgg
     1081 ccggcgaaac caccggcatc tcctacgaag acgccaaggg ccagtggcgc gaggaaatcg
     1141 cggccggcga cgatcggccc acgacccacg tcgtcgatag tcagtaacgg cgtttttcgg
     1201 ctggcccctg tcttgctcct gagatgagcg acgagccatt tcgagacagg gtccacgcca
     1261 atgttcgcca agaagaaaga cacctccgcc ccggtcgccc tggcgccgct ggaacctgcc
     1321 cgcgcgccgg ccgcccaggc cgccgcgccg gcgtccgcgc cccgacccaa gccggcctcg
     1381 atgatcgccc agggtgtcac catcaagggt gacgtcgtcg gcgacggcga gctgcatctg
     1441 gactgcgtgg tgctgggcga catccgggtc ggcaagctga tcctgggtcc caacagccgg
     1501 gtcgagggaa gcgtcaccgc tcaggtgatc gagatccacg gcaatgtgct gggcagcgtc
     1561 accgcccagg cggtgcgcct gtacggcacg gcgcggatcg atggcgacat cacccacgag
     1621 cagctggcca tggaaaccgg cgccgagttc cagggccgca gcctgaagtt ccagcgcccc
     1681 gctccggtcg ccgcccagcc cgcaagccac gttccggcca tgccgcagag cgcgcccgcc
     1741 tatagcgcct aggggtggtc ggtctggccg cccttctgca agctggctga cgtcatcggg
     1801 gtccctccgc cctccctctg ggaaacggcg gaaggacccc gcggctcccc gccgttttgt
     1861 ggggggacgg gacggcgggt gcgctcaggc cggcctgact ttcgtcaccc ggacaattgc
     1921 gtgcaaacgc agctacgcgc cgccccgccc cctggatgcg cggaaatgtc gcgctgatcg
     1981 tcgatcaagg atcagccggc ggaattggcc atttttcgca ggcggcgagc caggtcttca
     2041 ggccggtcag cgatctggtc gaacagggcc tggacctcgg cttcgtggaa gccggccgcc
     2101 cgcagggtgc gccaggcgcc cgccagcaca gcctcggcaa tctcgatgcg atcctcgccg
     2161 gacaccggcc catcatgccg actggagaag gcgcccgcga aggcggaacc gggcgcgaca
     2221 gacaggatcg aggcggcgga catcggcgtg ctccctttgg atggcgcacc acaatgttcc
     2281 tattttgttc gcaatgacaa gccctctcgg cgcgccagaa aaaaagcccc ggagcggggg
     2341 ctccggggct ttcctcaatc gcgggcgtta gacggctcat gaccattgga agcattcgtc
     2401 cagattttaa gccctggcgc tagagacgaa acgctcacca acgggaacgt tcacattcac
     2461 ggtctagatt agcggcagac cgtccgctca tagacagcgc cgctgcggcg gtcatactct
     2521 cgggtcgtcc agcaatcttc gcgatatccg ccattatggg gccgcccata gtagcggggc
     2581 ggcggagcgt aatcataacc attgctatag ccattgccat agccataccc cgaaccggcg
     2641 ccgtaaccat tgccatagcc gcgactgggg cgggcatagc ggtcattgcc attgctgctg
     2701 ctggcgatcg ccgcgccaag cgccaagcct atcacgccgg ccacgatcgc cgcctcggcg
     2761 cccttgttgt cgttgcggtg atggcgatga tgacgacgcg aatccgccga ggccgtggtc
     2821 gccgcggcga ccatcgacag cgcggcaagg ccggccgcga catggccaat cgtggtgctg
     2881 atcttgcggg ccatcggccg ctccccttcg tctcgatccg cccggcggcg gtcgaatgat
     2941 accgtgaatg caatctcgcg ccgcctgcct gaaccgcacc tgaacggcgc gagacccgat
     3001 aaacggcgcc ggtttattca ctatttgccg ccaagctcgc gtggccggat gagctgagtg
     3061 tcgcccggcg catcgaccag cggagactcc catgaccagc agcgtggcgt acaagacggg
     3121 cgaagccctt caggttctgt cgttcgaggt cggaacccag acctattgcg tgccggtcaa
     3181 ttcggttcgc gagatccgcg gcgtcacccc gcccacccag ctgcccgacg ctccgcccta
     3241 tgtgcgcggc gtgatcaacc tgcgcggcca ggtcatgccg gtcatcgacc tgtcggcccg
     3301 cctgggcaag ggcgtggccg aggacggtcc ccgccaggtc atcatcgtca tcgaaagcgg
     3361 cgacgacgtc gccggcctgc tggtcgacgc ggtgtgcgac agcttcatcg tcgacggcga
     3421 ccagatcaat ccgcccccct cgatgggcga agagaactcg ccgctggtct cggccgtgat
     3481 cacctcggac accggcatga ccgtgctgct gtcggtcggc cacatcattc ccgagcgcgc
     3541 cgcccccgcc atcgcggcct gagcaaagcc gcaacacttc gaacgattgt tcggataagc
     3601 ccttccgctc gcccccgggg gaacgctaga ctcgctccaa atctcaggga gtgagcgaat
     3661 gaaggcggcc gtgctgcgcg aggtgggcaa gcccctccag atcgaaaccg tgtccatcgg
     3721 caagccgggc ccgcgcgagg tgctgatccg caccaaggcc gccggcgtct gccactccga
     3781 cctgcacttc gtcgaaggct cctaccccca ccccctgccc gccgtgctgg gccatgagag
     3841 cgccggcatc gtcgaggcgg tgggctccga ggtccgcacg gtgaaggtgg gcgaccacgt
     3901 catcacctgc ctcaacccct attgcggcca ctgcgaggtc tgcctgacag ggcacatgaa
     3961 cctgtgcatc agccccgaga cccgccggtc caagaccgac gaaccgcgcc tgttcaagga
     4021 agacctgtcc ggatctggcg acaccaagat ggcccagttc ctgaacctgt cgtccttcgc
     4081 cgagatgatg ctggtgcatg agcacgcctg cgtcgccatc cgccgcgaca tgccgtttga
     4141 cctggccgcc ctgatcggct gctcggtcat gacgggcgta ggggcggtga tccatacctc
     4201 cagcgtccgt ccgggcgaga ccgtggccgt gatcggctgc ggcggcgtgg gcctggccac
     4261 catcaatggc gcggccatcg ccggcgccgg ccgcatcatc gccatcgacc gcgtggcctc
     4321 caagctggag ttggccaaac agttcggggc caccgacctg atcaacgccg acgacggcgt
     4381 ctgctcgggc aaggaggtcg acgacgacgg cgacgtggtc aaacaggtgc aggaactgac
     4441 cggcggcggc gtgcagcaca gcttcgaggc catcggcctg aaggtcaccg ccgagcagtc
     4501 cttcaagatg ctgcgccggg gcggcacggc caacatcatc ggcatgatcc cggtcgggac
     4561 caagatcgag ctgaccggct ccgacttcct gggcgagcgg cgcatccagg gcagctatat
     4621 gggctccaac cgcttcccgg tcgacatgcc ccggctggtc gacttctaca tgtcgggcaa
     4681 gctgaagctg gaccagatga tctcgcggcg gatcaagctg gaagagatca attcggcgtt
     4741 cgacgagctg aagcgcggtg aactggcgcg gtcggtgatc gtgtttgact aagccccgcc
     4801 gggacccgta gccccaaaaa cgaaggtggg gagaggccgg cgctcgccgg tccctccccc
     4861 ttcgacagac gcgcgctcag gccttggccg gctgaacctg gcgggttttc cagagcgacc
     4921 agccgacgcc ggcggccagg atggcgaagg tgatcgacag cgacagggcg gcggggaact
     4981 tctcccagcc cagagcgtcg gcgatgaaga tcttgccgcc gatgaacacc agcaacaccg
     5041 ccagggcctg tttcagatag gcgaagcgat gcaggaccgc ggccagcgcg aaatacaggg
     5101 cgcgcaggcc aaggatcgcc atgatgttgg aggtgtagac gatataggga tcggtggtga
     5161 tggcgaagat cgccggcacc gagtcgacgg cgaacaccag gtcggccagt tcaaccagca
     5221 ccagggccag gaacaggggt gtcgccagac gggcgacctt gccggtgcgc ggatcaacgc
     5281 cctgaacaaa gaagcgatcg ccgtgcagct tgtcgctgac gggcatgacg cgccgcagcc
     5341 atttcagggc ggggttggtg ttcaggtcca ggggtttgtc gccggcaaac agcatcttca
     5401 cgccg
//
LOCUS       DAOMEO010000046         5393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1957_length_5393_cov_3.059386, whole genome shotgun sequence.
ACCESSION   DAOMEO010000046 DAOMEO010000000
VERSION     DAOMEO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_5393_cov_3.059386"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="PLH31_01815"
     CDS             complement(<1..90)
                     /locus_tag="PLH31_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQR88014.1"
                     /translation="MDRLEKLCIEKGMRMTDQRRVIARVLSAAD"
     gene            complement(136..588)
                     /locus_tag="PLH31_01820"
     CDS             complement(136..588)
                     /locus_tag="PLH31_01820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR88015.1"
                     /translation="MILRPVGLEACFILADLHDRAFERPWDAMAFEDLLKSPGVFAVL
                     GEAGEPGVEKGFILCRAIAGEAEILTIGVDPAARRRGWGGALVEMAAGIASETGSEAM
                     FLEVAADNAGAIALYAATGFARVGLRKGYYPHQDGARDAIVMRRALNS"
     gene            complement(585..1220)
                     /gene="tsaB"
                     /locus_tag="PLH31_01825"
     CDS             complement(585..1220)
                     /gene="tsaB"
                     /locus_tag="PLH31_01825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQR88016.1"
                     /translation="MILSIDTCLGASAVAILDGDRVLAARSEPMTRGHQERIGVLARE
                     LALEAGVTFSDLTRIGVTVGPGSFTGLRVGLAFAKGLATALSIPCVGVSTLEALAFGT
                     PGFVAAVLDARMSQVYLQIFDRGTALMAPDALDLGSAAARLAELHGGQAVTLIGSGAP
                     LLAEAFPKATVLTPVSPDPVAVAKLAAAKPAPAHSPRPLYLRAPYATLPAA"
     gene            complement(1226..>1522)
                     /locus_tag="PLH31_01830"
     CDS             complement(1226..>1522)
                     /locus_tag="PLH31_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1131 domain-containing protein"
                     /protein_id="HQR88017.1"
                     /translation="GDLGAIRLEGGDVRGPRDETLLTKWSALGLDIAHCRAGEGRDVN
                     TVVCVRPEAPNVQYLIGVRGWTQGGLPTVETLNAKGQLNGLVWRPIEGAAVGSA"
     assembly_gap    1523..1532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1533..1866)
                     /locus_tag="PLH31_01835"
                     /pseudo
     CDS             complement(1533..1866)
                     /locus_tag="PLH31_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515433.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1131 domain-containing protein"
     gene            complement(1983..2570)
                     /locus_tag="PLH31_01840"
     CDS             complement(1983..2570)
                     /locus_tag="PLH31_01840"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HQR88018.1"
                     /translation="MAKLDAPALAQLFTEARTRNGWKTDPLPESLLRELYDLVKFGPT
                     AANSTPARFVFVTSPEAKAKLAALSSGSNGPKILQAPATVIVGYDLDFPETLDKLFPH
                     APGAKTWFADPVAKEWGAFRNSSLQGGYFILAARALGLDVGAMSGFDNAGVDAEFFAG
                     TNIKSNFIASIGYGTDEGLFPRGPRLAFEEAAKII"
     gene            complement(2661..3230)
                     /locus_tag="PLH31_01845"
     CDS             complement(2661..3230)
                     /locus_tag="PLH31_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQR88019.1"
                     /translation="MFIQTETTPNPEVLKFLPGRDVLGEGAREFKTADEGESSPLAKA
                     LFQLGDVTRVFFGPDFLTVTKDPDAEWPHLKAPILAAIMDHFTSGRPLLLDATESGHD
                     DDGVYDEESSQIVAEIKELLDTRIRPAVAQDGGDIVFSRFEPATGVVWLHMRGACSGC
                     PSSSATLKSGVENMLKHYVPEVTRVEQTL"
     gene            3353..4624
                     /locus_tag="PLH31_01850"
     CDS             3353..4624
                     /locus_tag="PLH31_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR88020.1"
                     /translation="MSDGSIDLSKAALSAAAWSTPLEDFHVADPALFQADAMWPYFER
                     LRKEDPVHWSRGNEEVGPYWSVTRYNDIMAVDTNHQVFSSDAHLGGITIRDFDEDFVL
                     PMFIAMDPPKHDIQRKTVSPIVSPQNLAKLEGLIRERVNLILDDLPIGEPFDWVDRVS
                     IELTTQMLATLFDFPWEERRKLTRWSDVATASPESGIVESEDARRAELLECLAYFTNL
                     WNERVNAEEPGGDLISMLAQGEATKNMPPMEYLGNIILLIVGGNDTTRNSLTGGLYAL
                     SKNPEQEAKLRANPELIPSMVSEIIRWQTPLAHMRRTALQDVELAGKQIKKGDKVVMW
                     YVSGNRDDTVIEDADSFIIDRENPRRHLSFGFGIHRCVGNRLAEMQLKIVWEEILKRF
                     PKIEVLDEPKRVYSSFVKGYETLTVRIPERV"
     gene            complement(4792..>5393)
                     /locus_tag="PLH31_01855"
     CDS             complement(4792..>5393)
                     /locus_tag="PLH31_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQR88021.1"
                     /translation="LIYKATHVPVGEDQKQHLELTRDIAAKFNHDFAAPNFFPQPDPL
                     IQGPGARIMSLRDGSAKMSKSDPSDYSRINLTDDADAIATKVKKARTDPEPLPETLEE
                     LATRPEADNLVGIFAALDGRTKAEVLADYAGKGFGTFKPALAELAVEKLAPVGERMRS
                     LLADPAEIDRILARGAERAREVAAPTVAEVKTLVGFWGA"
BASE COUNT          918 a         1775 c         1831 g          859 t
ORIGIN      
        1 gtcggcggcc gacagcacgc gggcgatgac ccgacgctgg tcggtcatgc gcatgccctt
       61 ctcgatgcag agtttttcga gtcgatccaa ggcggcgcct ccgtctgagt cgatggcagg
      121 ataggcggtc gagccctagc tgttaagcgc tcgccgcatg acaatcgcgt ccctagcgcc
      181 atcctgatgg ggataatagc ccttgcgaag gccaaccctg gcaaagccgg tggcggcata
      241 gagcgcaatg gcccctgcgt tgtcggccgc cacctccagg aacatggctt cggagccggt
      301 ttcggaggca atgccggcgg ccatctcgac cagggcgccg ccccagccgc gtcgccgcgc
      361 ggccgggtcg acgccgatcg tcaggatctc ggcctcgccg gcgatcgcgc ggcacaggat
      421 gaagcccttt tccacgcccg gctcgcccgc ctcgcccagc acggcgaaga cgccggggga
      481 tttcagcagg tcctcgaacg ccatggcgtc ccacggccgc tcgaacgcgc ggtcgtgcag
      541 gtcggcgagg ataaaacagg cctccaggcc cacgggacgc aggatcatgc tgcgggcagg
      601 gtggcgtagg gcgcgcgcag atagagcggc ctgggtgaat gggcgggagc cggtttggcg
      661 gcggccagtt tggccacggc gaccgggtcg ggcgaaacgg gcgtcagcac ggtcgctttc
      721 gggaaggcct cggccagcaa gggcgcgccg gacccgatca gggtcacggc ctgaccgcca
      781 tgcagttcgg ccagccgcgc ggcggccgag cccagatcca gggcgtcggg cgccatcagg
      841 gcggtcccgc ggtcgaagat ctgcagatag acctggctca tccgcgcatc cagcacggcg
      901 gcgacgaaac cgggcgttcc gaaggccagg gcctccagcg tgctaacccc gacgcagggg
      961 atcgacaggg ccgtggccag gcccttggcg aaggccaggc ccacgcgcag gccggtgaac
     1021 gagcctggcc ccaccgtgac gccgatccgg gtcaggtcgg aaaacgtcac gccggcctcg
     1081 agcgcgagct ctcgcgccag aacgccgata cgctcctggt ggccgcgggt catcggctca
     1141 ctgcgcgccg ccagcacgcg gtcgccgtcc aggatcgcga cggcgctcgc gcccaggcag
     1201 gtgtctatgg acaggatcat gggtgtcagg ccgagccgac ggccgcgccc tcgatgggtc
     1261 gccagaccaa gccgttcagc tgacccttgg cgttcagggt ctcgaccgtc ggcaggccgc
     1321 cctgggtcca gccgcgcacg ccgatcaggt actgcacatt gggcgcctcg gggcgaacgc
     1381 agaccacggt gttgacgtcg cgaccctcgc cggcccggca atgggcgatg tcgaggccaa
     1441 gcgccgacca tttggtcagc agggtctcgt ccctggggcc gcgcacatcg ccgccttcaa
     1501 ggcggatcgc gccgaggtcg ccnnnnnnnn nnccatcagc gggatctggt tctgctcgac
     1561 cgtgatgatc ggcgcggctg gccccggccc cgaatgcagg aaggcgctct tgaccaccgc
     1621 cttggggaac agcgccgcca gggtcggggc gtcgaaggcc gtcgcgctgg tgatcgggcc
     1681 cacgccctgg gcgctgacgg ccagcgggcc cccggcatgg ggcgtcacgg cgacggcgac
     1741 cgaggcgctt tggccctctg cggcgggcgg cggggcggct ccggcctcgt cggccttctt
     1801 ctccaccgga ccgcaggcgg ccaggagcag gatgggcagg gcgatgaagg cgaggcgctg
     1861 ggtcatgggc tggttttagc gcgtgtgcgt cacatgcaca aaacaaccgt cgcctccgga
     1921 cgccgaaacg accggaggtt caggttcgga cccgatcctt actcacgcgg accgggccaa
     1981 actcagatga tcttggcggc ttcctcgaag gcgaggcgcg ggccgcgcgg gaacaggccc
     2041 tcgtcggtgc cgtaaccaat ggaagcaatg aaattggact ttatgttggt cccggcgaaa
     2101 aattccgcgt cgaccccggc attgtcgaag cctgacatcg cgccaacgtc caggccgagc
     2161 gcccgggcgg cgaggatgaa atagccgccc tgcagcgagc tgttgcggaa cgcgccccat
     2221 tccttggcca ccggatcagc gaaccaggtc ttggcgcccg gagcgtgcgg gaacagcttg
     2281 tcgagggttt cggggaagtc gaggtcgtaa ccgacgatca ccgtggccgg ggcctgcagg
     2341 atcttggggc cgttcgagcc ggacgacagc gccgccagct tggccttggc ttccgggctg
     2401 gtcacgaaca cgaaacgggc cggggtcgag ttggcggccg tggggccaaa cttcaccagg
     2461 tcgtacagct cgcgcagcag gctctcggga agcgggtcgg tcttccagcc attgcgcgtg
     2521 cgcgcctctg taaacagttg ggcgagcgcc ggagcgtcga gcttggccat gtgcgatcct
     2581 cagaggggct atcagcaact aatctggttg cgatataggt cttcgacgcg cccgcgcaag
     2641 tcggggccgg ggcagaaaag ctagagcgtc tgctcgaccc gggtgacctc gggcacatag
     2701 tgcttgagca tgttctcgac gccggacttc agggtcgccg acgaagacgg gcagcccgag
     2761 caggcgccgc gcatgtgcag ccagaccacg ccggtggccg gctcgaacct gctgaaaacg
     2821 atgtcgccgc cgtcctgggc cacggccggg cggatgcggg tgtcgagcag ttccttgatc
     2881 tcggcgacga tctggctgct ttcctcgtca tagacgccgt cgtcgtcatg accgctttct
     2941 gttgcgtcga gcagcagggg ccggccgctg gtgaagtggt ccatgatggc cgccaggatc
     3001 ggcgccttca ggtgcggcca ctcggcgtcc gggtccttgg tcacggtcag gaagtcagga
     3061 ccgaagaaca cccgcgtcac gtcgcccagc tggaacaggg ccttggccag cggcgaggac
     3121 tcgccttcgt ccgccgtctt gaactcgcga gcgccctccc ccagcacgtc gcggccgggc
     3181 aggaacttca ggacctcggg attgggggtg gtttcggtct ggatgaacat ggtggagaaa
     3241 tggacctcgc cagccttcgg cgcaagatgc gcgggtgacc acaaagaaag gtgacacagg
     3301 cgtcattttc tggcgtgatg cggaaaacaa cgatccttgg gaggggtccg ccatgagcga
     3361 tggttcaatc gatctgagca aggccgcgct gagcgccgcc gcctggtcca cgccgctgga
     3421 agactttcac gtcgccgatc cggccctgtt ccaggccgac gccatgtggc cctatttcga
     3481 gcgcctgcgg aaagaagacc ccgtccactg gtccaggggc aacgaggagg tcgggcccta
     3541 ctggtcggtg acccgctata acgacatcat ggcggtcgac accaatcacc aggtgttctc
     3601 gtcggacgcc catctgggcg gcatcaccat ccgcgacttc gacgaggact tcgtcctgcc
     3661 gatgttcatc gccatggacc cgcccaagca cgacatccag cgcaagaccg tcagcccgat
     3721 cgtctcgccc cagaacctgg ccaagctcga aggtctgatc cgcgagcggg tcaatctgat
     3781 cctcgacgac ctgccgatcg gcgagccctt cgactgggtc gaccgggtgt ctatcgagct
     3841 gaccacccag atgctggcca ccctgttcga cttcccgtgg gaagagcgcc gcaagctgac
     3901 ccgctggtcg gacgtggcca cggcctcgcc ggaaagcggc atcgtcgaga gcgaggacgc
     3961 ccgccgggcc gagctgctgg aatgcctggc ctatttcacc aatctctgga acgagcgggt
     4021 caatgccgaa gagccgggcg gtgacctgat ctcgatgctg gcccagggcg aagccaccaa
     4081 gaacatgccg cccatggaat atctgggcaa catcatcctg ttgatcgtcg gcggcaatga
     4141 caccacccgc aactcgctga ccggcggcct ctatgccctg tccaagaacc ccgagcagga
     4201 agccaagctg cgggccaatc ccgagctgat cccctcgatg gtctcggaaa tcatccgctg
     4261 gcagacgccg ctggcccaca tgcgccgcac cgccctgcaa gatgtggaac tggccggcaa
     4321 gcagatcaag aagggcgaca aggtcgtcat gtggtacgtc tcgggcaacc gcgacgacac
     4381 ggtgatcgaa gacgccgaca gcttcatcat cgaccgcgaa aacccccgcc gccacctgtc
     4441 gttcggcttt ggcatccacc gctgcgtggg caaccgcctg gccgagatgc agctgaagat
     4501 cgtctgggaa gagatcctca agcgcttccc gaagatcgag gtgctggacg aacccaagcg
     4561 ggtctattcc agcttcgtga agggctatga gaccctgacg gtgcggatcc ccgagcgggt
     4621 ctgatttcct tctccccttg cgggagaagg tggcggctga aagccgacgg atgaggggtt
     4681 gcgcgaacgc ccctcatcca gccggtttcg aaccaaggcc gaaaggtctt tcgacccctc
     4741 atccggcgct gcgcgccacc ttctcccgca aggggagaag gaagaggcct tctaagcccc
     4801 ccagaacccc accagcgtct tcacctcggc cacggtcggg gccgcgactt cacgcgcgcg
     4861 ctccgcgccc ctggccagga tgcgatcgat ctcggccggg tcggccagca gcgaacgcat
     4921 acgctcgccg accggggcca gcttctcgac ggccagctcg gccagggccg gcttgaaggt
     4981 tccaaagccc ttgccggcgt agtcggccag cacttcagcc ttcgtccggc cgtccagcgc
     5041 cgcgaagatg ccgaccaggt tgtcggcctc gggacgggtc gccagttctt ccagggtttc
     5101 cgggagcggc tcaggatcgg tccgggcctt tttcaccttg gtggcgatgg cgtcggcgtc
     5161 gtcggtcagg ttgatgcggc tgtagtccga cgggtcagac ttgctcatct tggccgagcc
     5221 gtcgcgcagg ctcatgatcc gcgcgccggg accctggatc agggggtcgg gctgcgggaa
     5281 gaagttgggc gctgcaaagt cgtggttgaa cttggcggcg atgtcgcggg tcagttccag
     5341 atgctgcttc tggtcctcgc ccaccggcac gtgcgtggcc ttgtagatca gga
//
LOCUS       DAOMEO010000047         5363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1967_length_5363_cov_2.396383, whole genome shotgun sequence.
ACCESSION   DAOMEO010000047 DAOMEO010000000
VERSION     DAOMEO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1967_length_5363_cov_2.396383"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLH31_01860"
     CDS             <1..363
                     /locus_tag="PLH31_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HQR88022.1"
                     /translation="EAGRVVETGPVEDVFLHPASATTRRFVREADGEPAAEAVLAGRV
                     VRLTFRGEATYKPVLGQVARATGVDYSILGGRIHRLRETPYGQLTLALTGGDVDAAIA
                     RFQAEGVRVDPVLAGEGA"
     gene            363..1016
                     /locus_tag="PLH31_01865"
     CDS             363..1016
                     /locus_tag="PLH31_01865"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HQR88023.1"
                     /translation="MNNIDWAEIGQATLDTLTMLGGSMVLTVALGLPLGVILFLTGKG
                     QMLENRVANGLLSLLVNILRSVPFVILLIVMIPLTVALVGTSLGVQGAIPPLVAGAAP
                     FFARLVETALREVDKGVIEASFAMGARRRQVVLGALLPEAMPGIIAAATVTAIALVSY
                     TAMAGVVGAGGLGDLAIRFGYQRFQTDVMVVTVVLLLILVQALQMVGDTVVRRVSHR"
     gene            1026..1388
                     /locus_tag="PLH31_01870"
     CDS             1026..1388
                     /locus_tag="PLH31_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88024.1"
                     /translation="MTDETPLFIAPPPEHWDEDFAQTLIGKTLMLGLTFLDDDGELLE
                     RQQFFGVVIEANDQTGIVLDLLGEQDGDIYTLPPQTSAITAAPPGVTTLAGEAPDFVV
                     NWTIHGSPDEPANDEDQA"
     gene            complement(1379..1627)
                     /locus_tag="PLH31_01875"
     CDS             complement(1379..1627)
                     /locus_tag="PLH31_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88025.1"
                     /translation="MAKETVYIVQAYIAGRGKGLKAEQQIGCKDAAEARRKAERLAPL
                     REGVVAFAASADVELGDYDENPDILFKAGRLPFPFDQA"
     gene            1757..>1897
                     /locus_tag="PLH31_01880"
     CDS             1757..>1897
                     /locus_tag="PLH31_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR88026.1"
                     /translation="MVARRALVALSLAALTLAACGQKPAASSASSDTLTVAATAIPHA
                     EVL"
     assembly_gap    1898..1907
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1908..2566
                     /locus_tag="PLH31_01885"
     CDS             <1908..2566
                     /locus_tag="PLH31_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR88027.1"
                     /translation="PKLAEQGLKIEIKVFNDYVQPNTQVAEKRIDVSYFQTLPYLETL
                     HKDKGTKLIAIKGVHIEPIGAYSAKYKGIAQLPQGATVAIPNDASTEGRALLLLSRNN
                     VITLKDPANPLSSLKDIAANPKGLKVKTLEAATLPRVLNQVDLAIINTNYALDAKLNP
                     SKDALLIEDSKSPYVNYLVGRPDNKDDPRVAKLAAALTTPDVAAFMAQKYAGAVVPAF
                     "
     gene            2672..3364
                     /locus_tag="PLH31_01890"
     CDS             2672..3364
                     /locus_tag="PLH31_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR88028.1"
                     /translation="MLVLDVCSSSGLEDWSPDLQKAKVYERILLDLILGELAPGARLD
                     EQSLAARYEAGLAGVRDALGRLALEGLVIRRARSGTLVAPLDLVELRQGYEARALIEP
                     HCAGLAARHASKVEAEAIFDAFEGGEEAARNRDLPLLVAMDQRFHAAVARASQNLSLA
                     RILIPLQHKAARFWVFSFNGATEAELLSDIEQHRDVARVIASGDVEGARTAMMRVLNV
                     TPEVVQRPTVEA"
     gene            3543..>3617
                     /locus_tag="PLH31_01895"
     CDS             3543..>3617
                     /locus_tag="PLH31_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88029.1"
                     /translation="MSFRTLLFSVTALSMLAAMGARAET"
     assembly_gap    3618..3627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3628..>4502
                     /locus_tag="PLH31_01900"
     CDS             <3628..>4502
                     /locus_tag="PLH31_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR88030.1"
                     /translation="TQVADEVDAVLVLARDKAGLLEKQPSSTVFGIDKPLIETPRSAS
                     LVSDLTLERFGIETLDGISAVSPGTHTASFYGVPGALNIRGTLAENYFRGFKRVENRG
                     TYSTPIGGAARIEILRGPPTPVYGAGKVGGLVNFIPKSARDEGRFLAQPEGQVTATFG
                     SYNKKLVTGQFGAPVSLGAAEGGVYVYGEVEDSHSYYKGIYPRRQLIELSADFDLGNG
                     WSTAFGGMVFHSDGDVQTAGWNRLTQDLIDNRTYITGRDTSLVDADGNGRLTPNEVGF
                     YPFASALYIPYYGFP"
     assembly_gap    4503..4512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4513..>5363
                     /locus_tag="PLH31_01905"
     CDS             <4513..>5363
                     /locus_tag="PLH31_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88031.1"
                     /translation="ISPADFSKTTTGTLYFDLAKDLGDDRTLKLQLFHDRQDNERFVS
                     YGYPSDIDAKVSEARVTLALPTTVGAISTRTLVGASHRRFEGRRRESFNSGLIALDRR
                     DIAFGVTATDTIDSPFTDEPGGIGLRWENDNRSDWNQSGAFFTSDIEAGRWNLILGGR
                     YDHYEVESQDTGFLSYTVAGQQADDRNKATYNASLTYKAPFGLMPYIAYAKASALEMS
                     QAGDIAPSLVANGSWLSNSDLAETGVKFQLLNGTLVGSLAAYRQNRTQLTNTSPPTVQ
                     GTRAKGV"
BASE COUNT          889 a         1821 c         1707 g          916 t
ORIGIN      
        1 gaggccggac gcgtggtcga gaccggcccg gttgaggacg tcttcctgca tcccgccagc
       61 gccaccaccc gccgcttcgt gcgcgaggct gacggcgagc ctgctgccga ggccgtgctg
      121 gccggtcgcg tggtgcgcct gaccttcagg ggtgaggcca cctacaagcc tgtgctgggc
      181 caggtggccc gcgccacggg ggtcgactat tcgatcctgg gcggccgcat ccatcgcctg
      241 cgcgagacgc cctatggcca gctgaccctg gccctgaccg gcggcgacgt cgacgccgcc
      301 atcgcccggt tccaggccga gggtgtgcgg gttgatcccg tgctggccgg ggagggcgcg
      361 tgatgaacaa catcgattgg gccgaaatcg gccaggcgac cctcgacacc ctgaccatgc
      421 tgggcggctc gatggtgctg accgtggcgc tgggcctgcc gctgggcgtg atcctgttcc
      481 tgaccggcaa gggccagatg ctggagaacc gcgtcgccaa cggcctgctg tcgctgctgg
      541 tcaatatcct gcgctcggtg ccgttcgtga tcctgctgat cgtgatgatt ccgctgaccg
      601 tggcgctggt cggcacatcg ctgggtgtgc agggcgcgat cccgccgctg gtggcgggcg
      661 ccgcgccctt cttcgcgcgg ctggtcgaga ccgccctgcg cgaggtcgac aagggcgtga
      721 tcgaggccag tttcgccatg ggcgccaggc gtcggcaggt ggtgctgggc gcactgctgc
      781 ccgaggccat gccggggatc atcgccgcgg cgacggttac cgccatcgct ttggtgtcct
      841 acaccgccat ggccggcgtg gtcggggcag ggggcttggg cgatctggcg atccggtttg
      901 gctatcagcg cttccagacc gacgtgatgg tggtcaccgt ggtgctgctg ctgatcctgg
      961 tgcaggcgct gcagatggtc ggcgatacgg tggtgcgccg ggtttcgcat cgctagaaga
     1021 cgcccatgac cgacgaaacg cccctcttca tcgcgccgcc tcccgaacat tgggacgagg
     1081 actttgctca gaccctgatc ggcaagaccc tgatgctggg cctgacgttt ctggacgacg
     1141 atggcgagtt gctggagcgc cagcagttct tcggcgtggt catcgaggcc aacgaccaga
     1201 cgggcatcgt gctggatctg ctgggcgagc aggacggcga catctacacc ctgccgcccc
     1261 agacctcggc catcaccgcc gcgcctccgg gcgtgactac cctggcgggc gaggcgcctg
     1321 acttcgtcgt caactggacg atccacggct cgccggatga gccagccaac gacgaagatc
     1381 aggcctgatc gaacgggaac ggcagtcgac cggccttgaa caggatgtca gggttctcgt
     1441 catagtcacc cagctcgaca tcggccgagg cggcgaaggc cacgacgccc tcgcgcagcg
     1501 gggccaggcg ctcggccttg cggcgggcct ccgcggcgtc cttgcagccg atctgctgct
     1561 cggctttcag gcctttgccg cgaccggcga tataggcttg cacgatgtag acggtttcct
     1621 tggccatccg cactcaggcc gcttgttcgg aaggcgggtc aagcgatccc tctcgcctcg
     1681 atcaggaaaa ctgatttcgt caggcgacgg aaaccgggca aaaccctacg cctattaccg
     1741 ccttatggag ccgttcatgg tcgcccgtcg cgcactcgtc gccctcagcc tcgccgcctt
     1801 gacgcttgcc gcctgcggcc agaagcccgc cgcctcgagc gccagcagcg acaccctgac
     1861 cgtcgccgcc acggccattc cgcacgccga ggtgctgnnn nnnnnnnagc ccaagctggc
     1921 cgagcagggc ctgaagatcg agatcaaggt cttcaacgac tacgtccagc ccaacaccca
     1981 ggtggccgag aagcgcattg atgtcagcta tttccagacg ctgccatacc tggagacctt
     2041 acacaaggac aagggcacca agctgatcgc catcaagggc gtccacatcg agccgatcgg
     2101 cgcctattcg gccaagtaca aggggatcgc gcagctgccg cagggcgcca ccgtggccat
     2161 ccccaacgac gccagcaccg agggccgcgc cctgcttctg ctgtcgcgca acaacgtcat
     2221 caccctgaag gacccggcca atccgctgtc gagcctgaag gacatcgccg ccaatcccaa
     2281 gggcctgaag gtcaagaccc tggaggccgc caccctgccg cgcgtgctga accaggtgga
     2341 cctggcgatc atcaacacca actacgccct cgacgccaag ctgaacccgt ccaaggacgc
     2401 cctgctgatc gaggacagca agagccccta tgtgaactat ctggtcggcc gtcctgacaa
     2461 caaggacgac ccccgcgtcg ccaagctggc ggcggccctg accacgccag acgtggcggc
     2521 cttcatggcc cagaagtacg cgggcgcggt ggtgccagct ttctagctgg ctctggctct
     2581 ttgtgacatt tcaccttacc tatcagagca gaacgtctct gaaggagcct tgccggtgtc
     2641 accccttcca gcccaggacg cccgctcgcg aatgctggtg ctcgacgtct gttcgtcgtc
     2701 gggcctggag gactggtcgc cggatctgca gaaggccaag gtctatgagc ggattctgct
     2761 ggatctgatc ctgggcgaac tggcgcccgg cgcgcgcctg gacgagcaat cgctggcggc
     2821 gcgctacgag gcgggactgg ccggcgtgcg cgacgccctg ggccggttgg ctctggaagg
     2881 tttggtcatc cgccgcgccc gctcgggcac cctggtcgcg ccgctggacc tggtggaact
     2941 gcgtcagggc tatgaggcgc gcgccctgat cgagccgcac tgcgcgggtc tcgctgctcg
     3001 ccatgccagc aaggtcgaag ccgaggccat ttttgacgcc ttcgagggcg gagaagaggc
     3061 cgcgcgcaac cgcgacttgc cgctgctggt ggccatggat cagcgcttcc acgccgccgt
     3121 ggcgcgcgcc agccagaacc tgtccctggc ccgcatcctg atcccgctgc agcacaaggc
     3181 cgcgcggttc tgggtgttct cgttcaatgg cgcgaccgag gctgaacttc tttctgacat
     3241 cgagcagcac cgcgatgtgg cccgggtgat tgccagcggc gacgtcgagg gcgcgcgcac
     3301 tgcgatgatg cgcgtgctga acgtgacgcc cgaggttgtt cagcggccga cggtcgaggc
     3361 ctgaagctct gcattgatcg agaacaacct tcagtcggta ttgtgaggct ttccggccac
     3421 acccccttca aaataccaac tgaaatgtca gagagcattt tgacggaagc gcaatgatcg
     3481 ccagatggtg gggctaaccg cccggctatc cctcgcgccg ggctctatcg ggtcttgtcg
     3541 ccatgtcatt tcgaaccctg ttgttctccg tcaccgctct gtcgatgctg gccgccatgg
     3601 gcgcgcgggc cgagaccnnn nnnnnnnaga cccaggttgc cgacgaggtc gacgctgtgc
     3661 tggtgctggc gcgcgacaag gccgggctgc tggagaagca gccgtccagt accgtctttg
     3721 gcatcgacaa gccgctgatc gagacgccgc gttcggccag cctggtcagc gacctgaccc
     3781 ttgagcgctt cggtatcgaa acgctggacg gcattagcgc cgtctcgccc ggcacccata
     3841 ccgccagctt ctatggcgtg cccggcgcgc tgaacattcg cggcaccctg gccgagaact
     3901 atttccgcgg cttcaagcgc gtcgagaacc gcggcaccta ctccaccccc atcggcgggg
     3961 cggcccggat cgagatcctg cgcggcccgc cgacccccgt ctatggcgcc ggcaaggtcg
     4021 gcggtcttgt gaatttcatt cccaagtcgg cccgcgatga gggacgattc ctggctcagc
     4081 cggaagggca ggtcacggcg accttcggca gctataacaa gaagctcgtc accggccagt
     4141 tcggcgcgcc ggtcagcctg ggtgcggccg agggcggcgt ctatgtgtat ggcgaggtcg
     4201 aggacagcca cagctactac aagggcatct atccgcgccg ccagctgatc gagctgtcgg
     4261 ccgatttcga tctgggcaac ggctggagca cggctttcgg cggcatggtc ttccattccg
     4321 acggcgacgt gcagacggcg ggctggaacc gcctgaccca ggacctgatc gacaaccgca
     4381 cctacatcac cggtcgcgac accagcctgg tcgacgccga cggcaatggt cgcctgacgc
     4441 ccaacgaggt gggcttctac cccttcgcca gcgcgctcta cattccctac tacggcttcc
     4501 ccnnnnnnnn nnatatcagc ccggctgact tctcgaagac gaccaccggc acgctctatt
     4561 tcgatctggc caaggatctg ggcgacgacc ggacgctgaa gctgcagctg ttccacgacc
     4621 gtcaggataa cgagcgcttc gtctcctacg gctatccctc cgacatcgac gcgaaggttt
     4681 ccgaggctcg ggttacgctg gccctgccaa ccaccgtcgg cgcgatcagc acccgcaccc
     4741 tggttggcgc ctcgcatcgc cggttcgagg gccgtcgccg ggaaagcttc aacagcggcc
     4801 tgatcgccct ggaccgccgc gacatcgcct ttggcgtcac cgcgaccgac accatcgaca
     4861 gccctttcac cgacgagccg ggcgggatcg gcctgcgctg ggaaaacgac aatcgctccg
     4921 actggaacca gtcgggcgcg ttcttcacct ccgatatcga ggccggtcgc tggaacctga
     4981 tcctcggcgg tcgctacgac cactacgagg tcgagagcca ggatacgggc ttcctcagct
     5041 acacggtcgc tggccagcag gccgacgacc gcaacaaggc gacttataac gccagcctca
     5101 cctacaaggc tccgttcggc ctgatgccct acatcgccta cgccaaggcc tccgccctgg
     5161 agatgagcca ggcgggcgat atcgcgccgt ctctggtggc caatggctcg tggctatcga
     5221 actcggatct ggccgagacc ggcgtgaagt tccagttgct gaacgggacc ctggttggct
     5281 cgctggccgc ctatcgccag aaccgcaccc agctgactaa cacctcgccg cccaccgtgc
     5341 agggcacgcg cgccaagggc gtc
//
LOCUS       DAOMEO010000048         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1989_length_5302_cov_2.446731, whole genome shotgun sequence.
ACCESSION   DAOMEO010000048 DAOMEO010000000
VERSION     DAOMEO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_5302_cov_2.446731"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLH31_01910"
     CDS             complement(<1..446)
                     /locus_tag="PLH31_01910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR88032.1"
                     /translation="MNGHHKDYVAGLDGLRAASVSMVILGHVGLGDLIPGGMGVTIFF
                     FISGFLITRLLWAERDANGGVDLLRFYGRRTLRLLPEMLVLLLVLTFIVGPLLNQPMQ
                     PLQALAGLTYWTNYYIISGMGDCASCAVTGHLWSLAVEEHFYLVMPL"
     gene            complement(507..1601)
                     /locus_tag="PLH31_01915"
     CDS             complement(507..1601)
                     /locus_tag="PLH31_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQR88033.1"
                     /translation="MISAAGRLSRRSLLASSLALAACGRGASADPASPARSGPVPFLR
                     DLAPFPIGTEIVTDEFGRPGFDAAASSQFSQITAGFEMKMEYILQQDGSLRFEGGDRV
                     AAFCRANNQALHGHTLIWYIDRPAAFQKHVGDKRAFDRAYASYIQAVAGRYKGLARSW
                     DVVNEPVADDGVSLRESFWTENLGQEGHFIRAHEEAHAADPDALLFVNEYHLESKPHK
                     RRTFLKLVEDMLKRGVKIDGIGNQTHIDVDLPPGKITEAMRDLASLGLPIHLSEIDVS
                     FQTEGVSLKSKAEKQASQLRLYREAMESFIALPEKQQFAFTIWGVRDADSWLRNASWV
                     KDKTDTPLLFDDMGQPKETFWTLVDVLQGR"
     gene            complement(1598..2434)
                     /locus_tag="PLH31_01920"
     CDS             complement(1598..2434)
                     /locus_tag="PLH31_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQR88034.1"
                     /translation="MLSEWNQATSATQGVSIEGVRINLPTLDTAAEAAIRRAERGEGF
                     TLFTVNLDHLVKLTSNDAFRAVYRRATYVTADGWPVVWLAGRKQARLERATGADLVEP
                     LCRAAAERNLGVYFVGPGPKAQAGALEKLTQRFEGLKVAGAEAPMLPSGNGPAMLAAM
                     DLDAMAQRINASDARICFVSLGAPKQEILADALAARCPGVGFICVGAALDFISGQARR
                     APRWMQRGKLEWFWRLANDPRRLAVRYAECAMLFAVLALGVAGTQVGSPVTAAEPRGV
                     LR"
     gene            complement(2657..3106)
                     /locus_tag="PLH31_01925"
     CDS             complement(2657..3106)
                     /locus_tag="PLH31_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR88035.1"
                     /translation="MAAKLSGGGGGRYSLNQNSEINVTPFVDILLVLLIIFMVAVPMA
                     TVAIKVDLPPATIPPPNAPKPKEPVFISIQKSGALFIAEKQTSLDNLEFDLDAKFTST
                     GQAGPKDDQRVMIRADADVLYADFMGVLNQLQTAGWYKVGLINEDIR"
     gene            3330..4361
                     /locus_tag="PLH31_01930"
     CDS             3330..4361
                     /locus_tag="PLH31_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HQR88036.1"
                     /translation="MIFSFDAFVTAALFDRAGRAAFALGDGTVRLEADGGYVTVEAHG
                     GPIGGAVLSAVVHPSGHGLVTGGDDGRVVWSRIEGGEVVATVLAEVKGKWIEHLAASP
                     ASGLIAFTAGKEAHVRDTADPAFSRVFAHEKTVSGLAFDPKGRRLATAAYGGAQLWWA
                     KIAEQKPQILKWAGSHIAVAFSPDGKFLVSSMQENQLHGWRLSDGKDMRMGGYPAKIK
                     SMAFFDKGNLMVTAGANGAVVWPFAGASGPMGKEAAEIGFSRDSMVVRVAGVEALPVA
                     IAGQDNGKIWIAHMRNGRIETLKAEKGASISALAVSPDGQRLAWGDEDGEAGVITLPD
                     ISAPRQFAG"
     gene            complement(4366..5028)
                     /locus_tag="PLH31_01935"
     CDS             complement(4366..5028)
                     /locus_tag="PLH31_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQR88037.1"
                     /translation="MDVLDAVNRRMSVRAFKSDPVEAALLRELLEASARAPSGGNLQP
                     WRVQAIAGQPLEALRATMLQRVAAPDTPEYDVYPANLWEPLRSRRFQVGEDLYGALKI
                     PREDKMARLQWFASNGQGFGAPVLLFFSVDRRCGPPQWSDLGMFMQTLMLLAVERGLD
                     TCAQEFWSHYHQVVDQHVGLPEGHMLFSGMALGYRDEAAPVNTWRSRRDPFEVWGEMK
                     GF"
     gene            5134..>5302
                     /locus_tag="PLH31_01940"
     CDS             5134..>5302
                     /locus_tag="PLH31_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQR88038.1"
                     /translation="MKLLRNIFLALFIVLVAGPVLAVLVFRFVPPPVTPLMLIRAAEG
                     KGLDHRWRSIKT"
BASE COUNT          886 a         1699 c         1849 g          868 t
ORIGIN      
        1 agcggcatga cgaggtagaa gtgctcctcg accgccagcg accacaggtg tcccgtcacg
       61 gcgcagctgg cgcagtcgcc catgccgctg atgatgtaat agttggtcca gtaggtcagg
      121 ccggccaggg cctgcagcgg ctgcatcggc tggttcagca gcgggccgac gatgaaggtc
      181 agcaccagca gcagcaccag catctcgggc agcaggcgca gggtgcggcg gccatagaac
      241 cgcagcaggt cgacgccgcc attggcgtcg cgctcggccc acagcaggcg ggtgatcaga
      301 aagccgctga tgaagaagaa gatggtcacg cccatcccgc cggggatcag gtcgcccagg
      361 cccacatggc ccaggatcac catgctgacc gaggccgctc tcagcccgtc caggccggcg
      421 acatagtcct tgtgatgacc gttcatcagc gacccctccg cgcaacgtgc ggtcgagact
      481 aggggcgtag tcttaaaaaa ccgggatcag cggccctgaa gcacgtcgac cagggtccag
      541 aaggtttcct tgggctggcc catgtcgtcg aacagcagcg gcgtgtcggt cttgtccttg
      601 acccagctgg cgttgcgcag ccaggaatcg gcgtcgcgga cgccccagat cgtgaaggcg
      661 aactgctgct tttccggcag ggcgatgaag gattccatcg cctcgcgata caggcgtagc
      721 tggctcgcct gcttctcggc cttcgatttc aggctgacgc cttcggtctg gaacgagaca
      781 tcgatctccg acaggtggat cggcaggccc aggctggcca ggtcgcgcat ggcctcggtg
      841 atcttgcccg ggggcaggtc gacgtcgatg tgggtctggt tgccgatgcc gtcgatcttc
      901 acgccgcgct tcagcatgtc ctcgaccagc ttcaggaagg tgcgccgctt atgcggcttg
      961 ctctccagat ggtactcatt gacgaacagc agggcgtcag gatcggcggc gtgagcctct
     1021 tcatgggccc ggatgaagtg accttcctgg cccagattct cggtccagaa cgattcgcgt
     1081 aaggaaacgc cgtcatcggc gacgggctcg ttgaccacgt cccagctgcg ggccaggccc
     1141 ttgtagcgtc cggcgacggc ctggatgtag ctggcatagg ccctgtcgaa ggcgcgcttg
     1201 tcgccgacgt gcttctggaa ggcggccggc ctgtcaatgt accagatcag ggtgtggcca
     1261 tgcagggcct ggttgttggc acggcagaac gcggccacac ggtcgccgcc ctcgaagcgc
     1321 agggagccat cctgttgcag gatgtactcc atcttcatct cgaagccggc ggtgatctgc
     1381 gagaactggc tgctggcggc ggcgtcgaag ccgggccggc caaattcgtc ggtgacgatc
     1441 tctgtcccga tcgggaacgg cgccaggtcg cgcaagaatg ggacaggtcc gctgcgggcc
     1501 ggcgaagcgg gatcggccga ggcgcccctg ccgcaggcgg ccagggccag gctcgaggcc
     1561 agaagcgagc gtcgcgacag ccgtcccgcc gccgagatca tcgcagaacg ccgcgcggtt
     1621 cggcggcggt gactggcgag ccgacctggg ttccggccac gcccagggcc aggacggcga
     1681 acagcatggc gcattcggca tagcgtacgg ccaggcggcg cggatcattg gccaggcgcc
     1741 agaaccactc cagcttgccg cgctgcatcc agcgcggcgc gcggcgggcc tggccggaga
     1801 tgaagtccag agcggcgccg acgcagatga agcccacgcc cgggcagcgg gcggccaggg
     1861 cgtcggccag gatttcctgc ttgggcgcgc ccagcgagac aaagcagatg cgggcgtcgg
     1921 aagcgttgat gcgctgggcc atggcgtcga gatccatggc cgccagcatg gccggaccat
     1981 tgcccgaggg caacatcggc gcctcggcgc cggccacctt caggccctca aagcgctggg
     2041 tcagcttttc cagcgcgccg gcctgggcct tggggccagg acccacgaaa tagacgccca
     2101 gattgcgttc ggcggcggcg cggcacaggg gctcgaccag atcggcgccc gtggcgcgct
     2161 ccagcctggc ctgcttgcgg ccggccagcc acacgacggg ccaaccatcg gcggtgacat
     2221 aggtggcgcg gcgatagacg gcccggaagg cgtcgttcga ggtcagcttg accaggtgat
     2281 cgagattgac ggtgaacagc gtaaagcctt cgccgcgctc ggcgcgacgg atcgcggctt
     2341 cggccgcggt gtccagcgtc ggcaggttga tgcgcacgcc ttcgatcgaa acgccctggg
     2401 tggcggacgt cgcctggttc cattcgctga gcattcggct ctccctggtc tgtgcccggg
     2461 gcgccgttcc tgtccggaaa cgggacgccc aaggcctctt gggagggacg gtttcaagcg
     2521 tcgtgccgtg cgcgactggc ggctcgcccg ccgcgcgatt tgcggctcag gggaaaggcc
     2581 aaagaaaaag ccccggaagc ggcgcttccg gggctttctg cattttggtg cgccagaagg
     2641 cctggcgccg aagatcctag cggatgtctt cgttgatcag gccgaccttg taccagccag
     2701 cggtctgcag ctggttcagc acgcccatga agtcggcgta cagcacgtcg gcgtcggcgc
     2761 ggatcatgac gcgctgatcg tccttggggc cagcctggcc ggtcgaggtg aacttggcgt
     2821 ccagatcgaa ctcgagattg tcgaggctgg tctgcttttc agcgatgaac agcgcgcccg
     2881 acttctggat cgagatgaac accggctctt tcggcttcgg cgcgttgggc ggcgggatcg
     2941 tcgccggggg caggtcgacc ttgatcgcga cggtggccat cggcaccgcg accatgaaga
     3001 tgatcagcag aacaaggagg atgtccacga acggcgtgac gttgatttcg gaattctggt
     3061 tcagggagta gcgaccaccg ccaccaccag agagtttagc cgccatagcc tgactgtctc
     3121 ccaagggccg cgtcactcgc ggtcccgcaa ttgttaggtc gccacgtctg gctcagacgg
     3181 acgcggaagt aaagcccgac gtggcggttc gatggcaacc ccctttttga ctctttgtga
     3241 cgtaacgccg ttcgcatcgg acagggcgcc ggaggtcttc aggcgccatt cggctaggcg
     3301 ccagagccct gtcggggtat ggcgcgggca tgattttctc cttcgacgcc tttgtcaccg
     3361 cagccctttt cgatcgcgcc ggccgcgcgg ccttcgccct gggcgacggc acggtccggc
     3421 ttgaagctga cggcggctat gtcaccgtcg aggcccatgg cggtccaatc ggcggcgcgg
     3481 tcctgagcgc ggtcgtgcat ccgtcgggtc acggcctggt caccggcggc gatgacggcc
     3541 gggtggtctg gagccgaatc gagggcggcg aggtggtcgc cacggtcctg gccgaggtga
     3601 agggcaagtg gatcgagcat ctggccgcca gcccggcctc gggcctgatc gccttcaccg
     3661 ccggcaagga ggcccatgtc cgcgacaccg ccgacccggc cttttcccgc gtttttgccc
     3721 acgagaagac ggtgtcgggt ctggccttcg atcccaaggg ccggcgcctg gccacggcgg
     3781 cctatggcgg cgcgcagctg tggtgggcca agatcgccga gcagaagccg cagatcctga
     3841 aatgggccgg cagccacatc gccgtggcct tcagcccgga tggcaagttc ctggtctcgt
     3901 cgatgcagga aaaccagctg catggctggc gcctgagcga tggcaaggac atgcggatgg
     3961 gcggctatcc ggccaagatc aagagcatgg ccttcttcga caagggcaat ctgatggtca
     4021 ccgccggggc caacggcgcg gtggtctggc cgttcgccgg agccagcggc cccatgggca
     4081 aggaggccgc cgagatcggt ttctcacggg attcgatggt ggtgcgggtg gcgggcgtcg
     4141 aggccctgcc ggtggccatc gccggtcagg acaacggcaa gatctggatc gctcacatgc
     4201 gcaatggccg gatcgagacc ctgaaggccg agaagggcgc ttcgatcagc gccctggcgg
     4261 tgtcgcccga cggccagcgc ctggcctggg gcgacgagga cggcgaggcg ggcgtgatca
     4321 cgctgccgga tatcagcgcg ccacggcagt tcgccggtta ggcgatcaga accccttcat
     4381 ctcgccccac acctcgaacg gatcgcggcg agagcgccag gtgttcaccg gcgcggcttc
     4441 gtcgcgatag cccagggcca tgccggaaaa cagcatgtgg ccctcgggca ggccgacatg
     4501 ctggtcgacc acctggtgat agtgcgacca gaactcctgg gcgcaggtgt ccagaccccg
     4561 ctcgacggcc agcagcatca gggtctgcat gaacatgccc agatcgctcc actgcggcgg
     4621 gccgcagcgg cggtcgaccg agaagaacag cagcaccggc gcgccaaagc cctgaccatt
     4681 gctggcgaac cactggagac gcgccatctt gtcttcacgc gggattttca gcgcgccata
     4741 gaggtcctcg cccacctgga agcgccggct gcgcaggggc tcccacagat tggccggata
     4801 gacgtcgtac tccggcgtgt cgggcgcggc gacccgctgc agcatggtgg cgcgcagcgc
     4861 ttccaggggc tggccggcga tggcctgcac ccgccagggc tggagattgc cgcccgaagg
     4921 cgcccgggcc gaggcttcca gcagctcgcg cagcagggcg gcctccaccg ggtctgactt
     4981 gaaggcccgc accgacatgc ggcggttgac ggcgtctagg acatccatgc caaagctccc
     5041 tgagcaaggt cgttcatgcc cccgcgacag ggacctatcc agtcgctagc atccggccgc
     5101 agcgtcaaga caagcaggat ctgagggcga tacgtgaagc tgttgcggaa tatctttctg
     5161 gcgctgttca tcgtgctggt ggccggaccc gtgctggcgg tgctcgtgtt ccgcttcgtg
     5221 ccgccgccgg tgacgccgct gatgctgatc cgcgccgccg agggcaaggg cctggatcac
     5281 cgctggcggt cgatcaagac gg
//
LOCUS       DAOMEO010000049         5279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_1999_length_5279_cov_2.233155, whole genome shotgun sequence.
ACCESSION   DAOMEO010000049 DAOMEO010000000
VERSION     DAOMEO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_5279_cov_2.233155"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..163
                     /locus_tag="PLH31_01945"
     CDS             11..163
                     /locus_tag="PLH31_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQR88039.1"
                     /translation="MPTSEFPTPARRPPYSVLANAKVEKALGRSMPAWQDALDRYLKV
                     KGHRQT"
     gene            197..1066
                     /gene="rfbA"
                     /locus_tag="PLH31_01950"
     CDS             197..1066
                     /gene="rfbA"
                     /locus_tag="PLH31_01950"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQR88040.1"
                     /translation="MKGIILAGGSGTRLHPVTLGVSKQLLPVFDKPMIYYPISILMMA
                     GIRDIMIISTPRDLPLFQGLLGDGSGWGVNFVYAEQAQPTGLADAYRIGAEFVGNDPS
                     TLILGDNLFYGAGMGRMLEGAATRAQNGASVFAYLVKDPDRYGVVEFDDNNRAVSIEE
                     KPAQPRSNWAVTGLYMYDNKVVDYARDLKPSPRGELEITDINRIYLENGALSVERMGR
                     GFAWLDTGTHDSLLEASEFVRAIEHRQGLKIGCCEEVAYQNRWINTDQLLGLAHALQK
                     SPYGAYLRSLAES"
     gene            1308..>1646
                     /locus_tag="PLH31_01955"
     CDS             1308..>1646
                     /locus_tag="PLH31_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR88041.1"
                     /translation="MSLVGSFKAAALDLYRGVLLTPLWWRVSVEQTVTRYRRSLLGPV
                     WMASTTLATAFSLAIVFGSIFGGDAKDNLSFIMIGVVGWSITGGILPEIANTFVNAAG
                     VMQVRSLPLSF"
     assembly_gap    1647..1656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1657..2122
                     /locus_tag="PLH31_01960"
     CDS             <1657..2122
                     /locus_tag="PLH31_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR88042.1"
                     /translation="SFHAFQQMSKIFINFMHQVVGLWLVLMLMKLAVVPHWTLLVNLP
                     IALLTGLFVSIPLGMVSVRFRDINYMVGFIAQSLFMLTPVFWRRSQVPETMRWIVDLN
                     PFSHLLEIVRQPLMGAAAPIHDVNASLAFLGVAALLTLISLTLVRTRVVFWL"
     gene            2128..2874
                     /locus_tag="PLH31_01965"
     CDS             2128..2874
                     /locus_tag="PLH31_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR88043.1"
                     /translation="MTSSIELTDVTLDYNLYSVRAQSLRNTVLNMAVGGRLIQAGKDV
                     TYVRALSNVSLKLNEGDRLAVIGHNGSGKTSLLKILAGIYSPTLGKVDVRGRISSMIS
                     ISVGLDIEASGLENIRNLAMMQMMSDREIKRRMPAIVEFSGLGAFVHMPLKTYSAGMV
                     ARLTFSVATQVDADILIMDEWISAGDADFQKRAADRLNNMVDSAKIVVLATHDYQLAA
                     KVCNKVMTMESGVPTFLGTTEEWLERSSAA"
     gene            2958..>5279
                     /locus_tag="PLH31_01970"
     CDS             2958..>5279
                     /locus_tag="PLH31_01970"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HQR88044.1"
                     /translation="MLFRLTRAEQPGFDDVASVRVTLKIRAIEGHIEAPSLLIDWGDG
                     LALATPVALKRLGEDAYVVVAHTNAGALSQIVLRPSAAPCLFELTAFQVEPAAEPAPG
                     APRLSAPRRLLRAALRGLSPGAARAIRISLRTLRDPKSTIRLARQTIRNRRLDPWRES
                     YQHAFNVARHAFSPHYAAPALNPPPRADDAPRVLAFYLPQYHPFPENDQWWGKGFTEW
                     TNVSKATPQFVGHYQPRLPGELGFYDLRVPEVLQAQARMARNAGVDAFCFHYYWFGGK
                     RLLERPLDAFVADPEIDLPFALCWANENWTRRWDGDESDVLMAQDHSPEDDLALLDDL
                     ARYMASPRYVRVGGKPLLIVYRPDTLPDAAATLERWRKRARELGIGELFILCTNAFGF
                     SAYRDAGFDGLVEFPPHALQKGEITDTVERLNLDFAGRVYDYDAVVEERIEALERRAD
                     PRIYPGVMPSWDNEARKPGCGNIFHNATPQRFHDWAGAALDCTTRLAPPDERLVFVNA
                     WNEWGEGTYLEPDRWFGHASAQALRNAVARRAPAPARDHPAVVVSHQSPRLHDAVMLF
                     HIFYADLIDDLAQRLATLRDGMDAIITFPEQWPATAVDALATAVPGAVLVPLPNHGRD
                     VVPFLTALEIARARGHTVFCKAHTKKSPHRSDGAAWRDRLLDGLMSPERAQAALAAFA
                     EDPRLGLLAPENARMLYSDPGVMTHNSEGVDYLSRYLNFRYSPDTAFPAGTMFWGRIA
                     AFEALVGHALTFEPELGRVDGTLAHAFERIVGCL"
BASE COUNT          848 a         1841 c         1675 g          905 t
ORIGIN      
        1 ggtctcgccg atcccgacgt ccgagtttcc gacccccgcc cgccggccgc cctacagcgt
       61 gctggccaac gccaaggttg aaaaggcgct gggccgatcc atgccggcgt ggcaagacgc
      121 tctggacaga tatctgaagg tcaaaggtca ccggcagacg tgaccggtcg cgcggggcag
      181 tagaggtagt atttcgatga agggcatcat tctggcgggc ggctcgggca cgagactgca
      241 cccggtgacc ttgggcgtca gcaagcagct gctgccggtc ttcgacaagc cgatgatcta
      301 ttatccgatc tcgatcctga tgatggcggg gatccgcgac atcatgatca tcagcacgcc
      361 ccgcgacctg ccgctgttcc agggcctgct gggcgacggc tcgggctggg gcgtcaattt
      421 cgtctatgct gagcaggcgc agcccaccgg cctggccgac gcctatcgca tcggcgcgga
      481 gttcgtcggc aacgacccct cgaccctgat cctgggcgac aacctgttct acggggccgg
      541 catgggccgg atgctggagg gcgcggcgac ccgcgcccag aacggcgcct cggtcttcgc
      601 ctatctggtc aaggatcccg accgctacgg ggtggtcgag ttcgacgaca acaaccgcgc
      661 cgtcagcatc gaggaaaagc cggcccagcc gcgctccaac tgggcggtga ccggcctcta
      721 catgtacgac aacaaggtcg tcgactatgc ccgcgacctg aagccctcgc cgcgcggcga
      781 gctggaaatc accgacatca accgcatcta tctcgaaaac ggcgccctgt cggtcgagcg
      841 gatgggccgg ggctttgcct ggctcgacac cggcacccac gacagcctgc tggaagcctc
      901 cgaattcgtg cgggccatcg agcaccgcca gggcctgaag atcggctgct gcgaggaggt
      961 cgcctaccag aatcgctgga tcaataccga ccagctgctg ggcctggcgc acgccctgca
     1021 gaagtcgcca tacggcgcct atctgcgctc gctcgcggaa agctgatcgc gggtctttct
     1081 agccgcgctg gaggccactg gcggccgctg tttcgcgcgc tcgaatggcc taaacaccca
     1141 gaagaatcag cggagtcgcg ccgccggatg agatgtttcc caagcttcca aggcctgtta
     1201 agaagacgcc cgcttcgacg gagcgaggtt tcggggcgca cccttcgccg ccagcctgtt
     1261 tgcgagagtt tccggatttg tctgccgtca acaggaatgg cttctgaatg agcctcgtcg
     1321 gaagttttaa ggccgccgcg ctggatctgt atcgcggcgt gctcctgacg cctctgtggt
     1381 ggcgggtcag cgtcgagcag accgtaacgc gctaccggcg cagccttctg ggtccggtct
     1441 ggatggccag caccaccctg gcgacggcct tttccctggc catcgtgttc ggctcgatct
     1501 tcggcggcga cgccaaggac aacctgtcct tcatcatgat cggcgtggtc ggctggtcga
     1561 tcaccggcgg tatcctgccc gagatcgcca acaccttcgt caacgccgca ggcgtgatgc
     1621 aggtgcgcag cctgccgctg agctttnnnn nnnnnngagc tttcatgcct tccagcagat
     1681 gagcaagatc ttcatcaact tcatgcacca ggtcgtcggg ctgtggctgg tgctgatgct
     1741 gatgaagctg gccgtggttc cgcactggac gctgctggtg aacctgccga ttgcgctgct
     1801 gacgggtctg ttcgtcagta ttccgctcgg catggtctcg gtgcgtttcc gcgacatcaa
     1861 ctatatggtc ggctttatcg cccagagcct gttcatgctc acccccgtat tttggcgccg
     1921 atcacaggtt cccgagacaa tgcgctggat cgtcgacctc aatccgttca gccacctgct
     1981 tgagatcgtc cggcaaccgt tgatgggcgc cgccgcgccg attcacgatg tcaacgcgtc
     2041 gctggccttc ctcggcgtcg ccgcgctgtt gacgctgatc agcctcaccc ttgtccgcac
     2101 ccgcgtcgtg ttctggctgt aggcgacatg accagctcca tcgaactcac cgacgtcacg
     2161 ctcgactaca acctgtacag cgtgcgcgcg cagtcgctgc gcaacaccgt gctgaacatg
     2221 gccgtcggcg gccgactgat ccaggccggc aaggatgtga cctatgtccg ggcgctttcg
     2281 aacgtcagcc tgaagctgaa cgaaggcgac cgcctggccg tgatcggcca caacggctca
     2341 ggcaagacct cgctgctgaa gatcctggcc ggcatctact cgccaaccct gggcaaggtg
     2401 gacgtgcgcg ggcggatcag ttcgatgatc tcgatctcgg tcgggctcga catcgaggcc
     2461 tcgggcctgg agaacatccg caacctggcc atgatgcaga tgatgtcgga ccgcgagatc
     2521 aagcgccgca tgccggccat cgtcgagttc tccggcctgg gcgcctttgt gcacatgccg
     2581 ctgaaaacct attcggccgg catggtcgcg cgcctgacct tctcggtcgc cacccaggtc
     2641 gatgccgaca tcctgatcat ggacgagtgg atcagcgccg gcgacgccga cttccagaag
     2701 cgggccgccg accgcctgaa caacatggtc gactcggcca agatcgtcgt tctcgccacc
     2761 cacgactacc aactggccgc caaggtctgc aacaaggtca tgaccatgga atcgggcgtg
     2821 ccgaccttcc ttgggaccac cgaggaatgg ctcgagagaa gcagcgccgc gtgagcgggc
     2881 ggcttcccgc gccccggaag gctgtcgagc cgaccggatc gcctggccag ttcagggcca
     2941 cgacctcaga cgcggagatg ctgtttcgcc tgaccagggc cgagcagccc gggttcgacg
     3001 acgtggcgtc ggtccgcgtg acgctgaaaa tccgcgcgat cgagggccat atcgaagccc
     3061 ccagcctcct gatcgactgg ggcgacggcc tcgccctggc gacgcccgtg gcgctgaagc
     3121 ggttgggcga ggacgcctat gtcgtggtgg cccacaccaa tgccggcgcc ttgagccaga
     3181 tcgtgctgcg cccatccgcc gcgccctgcc tgttcgagct gaccgcgttt caggtcgagc
     3241 cagcggccga accggcgccc ggcgctccga ggctttcggc gccgcgccgg ctgctgcgcg
     3301 ccgcgcttcg cggtttgtcg cccggcgccg cacgcgcgat ccggatcagc ctgcgcacgc
     3361 tgcgcgatcc gaagagcacg atccgcctgg cccgtcagac gatcagaaac cgccgcctgg
     3421 atccctggcg cgagtcctac cagcacgcct tcaatgtggc gcgccacgcg ttcagccccc
     3481 actacgccgc gccggccctg aacccgccgc cgcgcgccga cgatgcgccc agggtgctgg
     3541 ccttctacct tccgcagtac catccgttcc ccgagaacga ccagtggtgg ggcaagggct
     3601 tcaccgagtg gaccaatgtc agcaaggcga cgccgcagtt cgtgggccac tatcagccgc
     3661 gcctgcccgg cgagctgggc ttctacgacc tgcgggtgcc cgaggtgctg caggcccagg
     3721 cccggatggc ccgcaacgcc ggcgtcgacg ccttctgctt ccactactac tggttcggcg
     3781 ggaagcgcct gctggagcgt ccgctagacg cctttgtggc agaccccgag atcgacctgc
     3841 cgttcgccct ttgctgggcc aacgagaact ggacgcggcg ctgggacggc gacgagagcg
     3901 acgtcctgat ggcgcaggac cattcgccgg aagacgacct ggccctgctc gacgacctgg
     3961 cccgctacat ggcttcgcca cgctatgtgc gggtcggcgg caagcccctg ctgatcgttt
     4021 atcgccccga caccctgccc gacgccgccg ccacgctgga gcgctggcgc aagcgggcgc
     4081 gcgagctcgg gatcggcgag ctgttcatcc tttgcacgaa cgccttcggc ttctcggcct
     4141 acagggacgc cggcttcgac ggcctggtcg agttcccgcc ccacgccctg cagaagggcg
     4201 agatcaccga cacggtcgag cgcctgaacc tggatttcgc cggccgggtc tatgactacg
     4261 acgccgttgt cgaggaacgg attgaggctc tggagcgtcg cgcggacccc cggatctatc
     4321 ccggcgtcat gccctcgtgg gacaacgagg cccgcaagcc cggctgcggc aacatcttcc
     4381 acaacgccac gccgcaacgc ttccacgact gggctggcgc ggccctggac tgcacgacgc
     4441 gtctggcgcc gccggacgag cggctggtct tcgtcaacgc ctggaacgag tggggcgaag
     4501 ggacctatct ggagcccgac cggtggtttg gccacgcctc ggcccaggcc ctgcgcaacg
     4561 ccgtcgcccg ccgcgccccg gcccccgcgc gcgatcaccc cgccgtcgtc gtctcgcacc
     4621 agagcccccg gctccacgac gccgtgatgc tgttccacat cttctatgcc gacctgatcg
     4681 acgacctcgc ccagcgcctg gcgacactgc gggacggcat ggacgccatc atcaccttcc
     4741 cggagcagtg gccggccacg gcggtggacg ccctggctac ggccgtcccc ggcgccgtgc
     4801 tggttcccct gcccaatcac ggacgcgacg tggttccgtt cctgacggcg ctggagatcg
     4861 cccgggcgcg cggccacacc gtcttctgca aggcccatac gaagaagtcg ccgcaccggt
     4921 cggacggcgc ggcctggcgc gatcgcctgc tggacggcct gatgtcgccc gagcgcgcgc
     4981 aggcggccct ggcggcgttc gccgaggacc cgcggctggg cctgctggct ccggaaaatg
     5041 cccggatgct ctacagtgac cccggcgtca tgacccacaa cagcgagggc gtagactatc
     5101 tttcgcgcta tctgaacttc cgctacagcc ccgacaccgc cttcccggcg ggcacgatgt
     5161 tctggggtcg catagccgcg ttcgaggcgc tggtcggcca cgcgctgact ttcgagcccg
     5221 aactggggcg cgtcgacggc accctggcgc acgccttcga gcgcattgtc ggttgcctt
//
LOCUS       DAOMEO010000050         5274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2001_length_5274_cov_2.705116, whole genome shotgun sequence.
ACCESSION   DAOMEO010000050 DAOMEO010000000
VERSION     DAOMEO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_5274_cov_2.705116"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLH31_01975"
     CDS             <1..498
                     /locus_tag="PLH31_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HQR88045.1"
                     /translation="IPRFDKVTDDRAPAADWERFDGPADVILLEGWCIGARPQSEAAL
                     IEPVNALERDEDPAGVWRRYANAALAGPYRALFDRIDLLVAMTAPDFPTVRVWRGQQE
                     QRLRDSLAAQGRDPALAMDEAALDRFVAHYQRLTAWIAEDLSPRADIAIRLDANRQPL
                     ELRGL"
     gene            complement(495..1043)
                     /locus_tag="PLH31_01980"
     CDS             complement(495..1043)
                     /locus_tag="PLH31_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88046.1"
                     /translation="MTRPVWDKPRRMPLLPRLFSGVIVVGILGAVTLSVIDLVAVEKA
                     NATLPPPTAAQMLVEKPRARAEWGHVRANLKARLNQSSPLDLGAVWATRAGRICGLVN
                     GRGSFGGLTGMVRFYSVDRVPVFHRDVDHEAFQYAWFECARDRWVRLREGSEEPGFCG
                     TELGKRRCYTVRIVDRVRTVED"
     gene            1439..2320
                     /locus_tag="PLH31_01985"
     CDS             1439..2320
                     /locus_tag="PLH31_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQR88047.1"
                     /translation="MSGNYEYEEEDGQEGHEDDNQNDTIYENEYDDNLTGDGEDYDED
                     EDEDDNDAEDDAGDVDKDDSLNGGDNDDHLDAGDGDDDVAGGLGDDDLHGGLGGDELH
                     GDAGDDTVHGDDGDDLLRGEDGNDHIDGGTGDDDLHGNAGDDVEAGGEGEDWVVGGQG
                     RDSLSGDQGDDIVLGNLGADTLSGGDGDDIVRGGQDNDQIDGGIGADYISGDRGDDTL
                     AGGAGADSFHFSSGSGRDVISDFSASSGDRLYLDDAGFYSVSQVGGDVVVTLDSGDHI
                     TLTGVDLATQPSDWIVA"
     gene            complement(2513..4423)
                     /gene="uvrC"
                     /locus_tag="PLH31_01990"
     CDS             complement(2513..4423)
                     /gene="uvrC"
                     /locus_tag="PLH31_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQR88048.1"
                     /translation="MSVVNDDIPASAVQDANALRGAALIKDEVSRLPDAPGVYRMIGE
                     ADEVLYVGKAKSLKKRVIQYAQGRFHTNRIAHMVDATRAMEFVTTRTEADALLLEINL
                     IKSLKPRFNVLLRDDKSFPEIMIRRDHEAPQLRKHRGAHAVKGDYFGPFASAWAVNRT
                     LNTLQKAFLLRSCSDSVYESRDRPCMLHQIKRCAAPCTGLVSIPDYQALVDQAEAFLR
                     GKSRAVMGQMAKEMEAAAEDLEFERAARLRDRIRALSAVAQESQINPETVEEADVVAL
                     HVEGGQACVQVFFFRAGQNWGNRAYFPRITGAAEEEGVSEEAQAMTAFLGQFYDDKPI
                     PRLILANIEPAESALLAEAFALKSGRKVEISVPKRGEKADLVAHALTNAREALGRKMA
                     EGSAQTKLLAGVCEAFGLDAPPERIEVYDNSHIQGTNAVGGMIVAGPDGFMKGQYRKF
                     NIKSTELTPGDDYGMMKEVLKRRFARLVKEEEEGQSDNRPDLVLVDGGKGQLDAALEI
                     MADLGVDDIAVVGVAKGPDRDAGLERFFMPDKTPFMLEPKSPVLYYLQRLRDEAHRFA
                     IGAHRTRRSMDLKKNPLDEIEGIGPGRKKALLHAFGSAKGVGRAGIEDLLKVEGVSRP
                     MAERIHAFFRKG"
     gene            complement(4443..4724)
                     /locus_tag="PLH31_01995"
     CDS             complement(4443..4724)
                     /locus_tag="PLH31_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR88049.1"
                     /translation="MKPVFVSPRAYADIRYLEAWLAERNTIAALKVGPLPFEAMMSLR
                     EFPERGRQAVNYRGRELNVPFGAHAYIIRYRVEPDRVIIATVHHSLERR"
     gene            complement(4781..4972)
                     /locus_tag="PLH31_02000"
     CDS             complement(4781..4972)
                     /locus_tag="PLH31_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88050.1"
                     /translation="MADGDITLTIDAALAERLKPRAEAAGQSIEEFAIHLLEEDATVW
                     TEVDAICDAVMAENTGIPL"
     gene            5032..>5274
                     /locus_tag="PLH31_02005"
     CDS             5032..>5274
                     /locus_tag="PLH31_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88051.1"
                     /translation="MTTRPLFVTPLYEASLAGDKDFESFIDDLHDACIAVAEDDEAGQ
                     VWAEKQGYLGYTPYASLDELPQRDSLFADLKKKLDKH"
BASE COUNT          907 a         1688 c         1738 g          941 t
ORIGIN      
        1 atcccgcgct tcgacaaggt cacggacgat cgtgcgccgg ctgcggactg ggagcggttc
       61 gacggaccgg ctgacgtcat cctgctggaa ggctggtgca tcggcgcgcg gccccagtcc
      121 gaagccgccc tcatcgaacc ggtcaacgcc ctggagcgcg acgaggaccc ggcgggcgtc
      181 tggcggcgct acgccaacgc cgccctggcc ggaccctatc gcgcgctgtt tgaccggatc
      241 gaccttctgg tcgcgatgac tgcgcctgac tttcccaccg ttcgcgtctg gcgggggcag
      301 caggagcagc gcctgcgcga cagtctcgcc gctcaggggc gagatccggc gctggccatg
      361 gacgaggcgg ccctcgaccg cttcgtcgcc cactatcagc gcctgaccgc gtggatcgcc
      421 gaagacctgt cgccgcgcgc cgacatcgcc atccgcctgg acgccaaccg ccagccgctc
      481 gagctgcggg gcctttagtc ttccaccgtc cgcacccggt cgacgatccg caccgtatag
      541 cagcggcgct tgcccagttc ggtgccgcag aagccgggtt cctccgaacc ctcgcgcagc
      601 cgcacccagc ggtcgcgggc gcattcgaac caggcgtact ggaaagcctc gtgatccacg
      661 tcacgatgga aaaccggaac ccggtcgacc gaatagaacc gcaccatgcc ggtcagcccg
      721 ccgaaactgc cccggccatt gaccagcccg cagattcgtc cggcgcgggt agcccatacc
      781 gcccccaggt ccaggggact ggactggttc agccgggcct tcagattggc ccgcacatgg
      841 ccccattcgg cccgggccct gggcttctcg accagcatct gcgcggcggt cggcgggggc
      901 agggtggcgt tggccttttc gacggccact aggtcgatga ccgacagcgt caccgcgccc
      961 agaatgccga ccacgatcac gcccgagaag aggcgcggca gcaagggcat ccggcgcggc
     1021 ttgtcccaga ccggtctcgt catcggcgtt ctccccagcg cgatagcggc agtctggaag
     1081 tcgcgtatcc gttttacaag tctaaaaaat tactgacgtg aatttctatg gcctcaaacc
     1141 tacgctctcc ccggcgaaag ttgggaagag cgggaagatt gggttagtcg ggccgcgttc
     1201 cgtctgccgc gccgtcttcg tcctcttcgt cgcgcgcgat ggatttcgag cgccgagccc
     1261 ccggcggttc gcaggctcga ccagctagaa ggcggcgggt gagaacgatt gtaggtgggg
     1321 gcgtctatgt cagaccaata gcgatcctca caaaatctct gcggcaaaat gaagcagggc
     1381 tatttcaggt tcagttcagc atgcggggct atcgtggcaa tctaaaatgg aggctgccat
     1441 gtccggtaac tatgaatatg aagaagaaga cggtcaggaa ggtcatgaag atgacaatca
     1501 aaatgacact atctatgaaa atgaatacga cgataaccta accggcgatg gcgaagatta
     1561 cgatgaagac gaagacgaag acgacaacga cgcagaagat gacgccggag acgtcgacaa
     1621 agacgacagt ctgaacggcg gcgacaatga cgatcacctt gacgctggcg acggtgacga
     1681 cgatgtcgcc ggaggccttg gtgacgacga tttgcacggc gggctaggtg gcgacgagct
     1741 tcacggcgac gcgggagatg acaccgttca tggcgacgac ggcgacgacc tgctgcgcgg
     1801 cgaggatggt aacgaccata tcgacggggg cacaggcgac gacgacctcc acggcaatgc
     1861 gggcgatgat gtcgaagccg gcggtgaggg tgaggactgg gtcgtaggcg gccaagggcg
     1921 ggatagcctt tcgggcgacc agggtgacga cattgtcctg ggcaatctcg gcgcggacac
     1981 gctctcgggc ggtgatggcg acgacatcgt tcggggcggc caggacaatg atcagatcga
     2041 tggcggcatc ggtgctgact atatttctgg tgacagaggc gatgatacgc ttgccggcgg
     2101 cgcaggagcc gacagtttcc atttctcctc agggagcgga cgcgatgtca tcagcgattt
     2161 ctccgcttca tccggcgatc gtctgtatct ggacgacgca ggcttctatt cggtcagtca
     2221 ggtcggcgga gacgtcgtcg tgaccttgga cagcggcgat catatcacgc tgaccggcgt
     2281 cgatttagcc actcagccct ctgactggat cgtcgcctga ttctcagagg ccgttgttaa
     2341 agctacaggc caaggaccgc gcatcgctca ggtgacgcgc ggtctggagc ccttcgtatc
     2401 cgagggaatg gaacaggtcg cgcggaaccg tcgcgtcagg tctgcggttt cggcgacaga
     2461 ccgctccctt cagcgacctt ccaaacgctc gctaccaggc ccgggcccgc cctcaccctt
     2521 tgcggaagaa cgcgtggata cgctcggcca tgggacggct gacgccctcc accttcagca
     2581 ggtcctcgat gcccgcccgt ccgacgccct tggctgagcc aaacgcgtgc aacagggcct
     2641 tcttgcggcc cggaccgatg ccctcgatct cgtcgagcgg gttcttcttc agatccatcg
     2701 agcggcgggt gcggtgggcg ccgatggcga agcggtgggc ctcgtcgcgc aggcgctgga
     2761 ggtagtagag caccggcgat ttgggctcga gcatgaacgg cgtcttgtcg ggcatgaaga
     2821 accgctccag cccggcgtca cggtccggac ccttggccac gcccacgacg gcgatgtcgt
     2881 cgacgcccag gtcggccatg atctccagcg ctgcgtccag ctggcccttg ccgccgtcga
     2941 ccagaaccag gtcggggcgg ttgtcgcttt gcccctcttc ctcttccttg accaggcggg
     3001 cgaagcggcg cttcagcacc tccttcatca tgccgtagtc gtcgccgggg gtgagctcgg
     3061 tgctcttgat gttgaacttg cggtactggc ccttcatgaa gccgtccggc ccggccacga
     3121 tcatgccgcc cacggcgttg gtcccctgga tgtggctgtt gtcatagacc tcgatccgct
     3181 cgggcggggc gtccaggccg aaggcctcgc acaccccggc cagcagcttg gtctgggcgc
     3241 tgccctcggc catcttgcgg cccagcgctt cgcgggcatt ggtcagggcg tgggccacca
     3301 ggtcggcctt ctcgccgcgc ttgggcacgc tgatctcgac cttgcgaccg ctcttgaggg
     3361 cgaaagcctc ggccagcagg gcgctttcgg cgggctcgat attggccagg atcaggcgcg
     3421 ggatcggctt gtcgtcgtag aactggccga ggaaggcggt catcgcctgg gcctcctcgc
     3481 tgaccccttc ttcttccgcc gcgccggtga tgcgggggaa gtaggcgcgg ttgccccagt
     3541 tctggccggc ccggaagaag aacacctgca cgcaggcctg gccgccctcg acgtgcaggg
     3601 ccacgacgtc ggcctcttcc acggtctcgg gattgatctg gctttcctgg gccacggccg
     3661 acagggcgcg gatgcggtcg cgcaggcggg cggcgcgttc gaactccaga tcctcggcgg
     3721 cggcttccat ttccttggcc atctggccca tcaccgcgcg ggacttgccc cgtaggaaag
     3781 cctcggcctg gtcgaccagc gcctgatagt cggggatgct gaccaggccc gtgcacggcg
     3841 cggcgcagcg cttgatctgg tgcagcatgc agggccggtc gcggctctca tagacgctgt
     3901 cgctgcacga gcgcagcagg aacgccttct gcagggtgtt gagcgtgcgg ttcaccgccc
     3961 aggcgctggc gaacggtccg aaatagtcgc ccttgaccgc atgggcgccg cgatgcttgc
     4021 gaagctgcgg ggcctcgtga tcgcggcgga tcatgatctc gggaaagctc ttgtcgtcgc
     4081 gcagcagcac gttgaagcgc ggcttcagcg acttgatcag gttgatctcg agcagcaggg
     4141 cgtcggcttc ggtgcgggtg gtgacgaact ccatggcccg cgtggcgtcg accatgtggg
     4201 cgatgcggtt ggtgtggaac cggccctgcg cgtactggat cacccgcttc ttcaggctct
     4261 tggccttgcc gacgtaaagc acctcgtcgg cctcgccgat catccggtac acgcccggcg
     4321 catcaggcag gcgcgagacc tcgtccttga tcagggcggc gccgcgcagg gcgttggcgt
     4381 cttggacggc ggaggcgggg atgtcgtcgt tcacgacgga catataggcg agtctgtcgg
     4441 cgtcagcgac gttccaggct gtgatgaacg gtcgcgatga tcacgcggtc cggttcgacg
     4501 cgatagcgaa tgatgtaggc gtgcgcgccg aaaggcacgt tcagctcgcg gccccgatag
     4561 ttcaccgctt gccggccacg ttcgggaaac tcccgtaggg acatcatcgc ctcgaaaggc
     4621 aacgggccta ccttcagagc cgctattgtg tttcgttccg ccagccacgc ttccagataa
     4681 cggatatccg cataggcgcg gggcgagacg aagacaggct tcatcgtggc ggaggcggac
     4741 cgtcaggctt gccccagccg ttcatccagg gccccagttc ttagagtgga atgccggtgt
     4801 tctcggccat taccgcatcg cagatcgcat cgacctcggt ccaaacggtg gcgtcttctt
     4861 cgagcagatg aatggcgaat tcttcgatgc tctggcccgc cgcttcggcg cgcggcttca
     4921 gccgctcggc gagtgcggcg tcgatcgtca gggtgatgtc accgtcagcc atgcctggat
     4981 gatccctcca agcccgtttg tcgtcaaccg cgcggggttg taagaggcgg catgaccacg
     5041 cgcccgctct tcgtcacccc gctctacgaa gccagcctcg ccggcgacaa ggacttcgag
     5101 tccttcatcg acgacctgca cgacgcctgc atcgccgtgg ccgaagacga cgaggcgggg
     5161 caggtctggg ccgagaagca gggctatctg ggctataccc cctacgcctc gcttgacgag
     5221 ctgccgcagc gcgacagcct gttcgcggac ctcaagaaga agctcgacaa gcac
//
LOCUS       DAOMEO010000051         5265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2004_length_5265_cov_1.920729, whole genome shotgun sequence.
ACCESSION   DAOMEO010000051 DAOMEO010000000
VERSION     DAOMEO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_5265_cov_1.920729"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLH31_02010"
     CDS             <1..261
                     /locus_tag="PLH31_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQR88052.1"
                     /translation="KAADLGAATWPRAETPTHYIVMGFNEDLDLAMKQALRGMINFIT
                     ARSALTPVQAYQFCSLAVDFRVTQTVNGEKGVHGMLEKGVLF"
     gene            401..2215
                     /locus_tag="PLH31_02015"
     CDS             401..2215
                     /locus_tag="PLH31_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQR88053.1"
                     /translation="MLLNGSEKADTLTGGADADTLYGYGGNDKLTGGAGDDVLAGMAG
                     NDTLLGGEGEDYILGGAGADTIDGGAGADWAAYEDAAAGVKVDLNLTGSQNTLGGGSD
                     RLISIENVYGSAFNDTLIGDAGVNYLAGGDGHDSLSGGKGEDNLFGGAGADTLLGGQN
                     DDYMQGGAGNDLIDGGVGIDWSAYDDATAGVTVNLTLTAAQNTGGGGVDTLVGVEYVY
                     GSAFADVLTGDANNNTLIGDLGDDSLSGGMGDDFLVGGAGDDVIDGGEGFDTIALVGD
                     RDGRIDLSVAIQDFSNYGLDTLISIEGVIGSDGRDSLAGNAAENYLAGKGGSDFLYAV
                     GGGDVLDGGEGDDFLRASKVGSGDYMSGGAGADTFFTMWGAVTLDGGDGADLFYVLRA
                     PGMIGTTVVNGGAGTDTLDLSSGYIPIGGAMVDLSIIGPQDIGAGQVLDISGVENLVG
                     GPGNDLLKGDVGANLIEGREGADTLIGGQGRDQLIGGTGIDTFVFAVGDSTTIDVTGS
                     GVDVISDFQAGDELRFSDAPAGFSYVEGSAGSFASALATVNSMLTYPSTFHEYIAFQV
                     DADVYVFSGHPDAGGAGLENVILLAGVNLDAVSYDSFL"
     gene            2359..3042
                     /locus_tag="PLH31_02020"
     CDS             2359..3042
                     /locus_tag="PLH31_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR88054.1"
                     /translation="MQKILLATVFLGLAACSPKPVAEKADAPAAAPAAKPAPANVPAG
                     AYRLDKTHASLVFTVNHLGFSNYTAAFADFDARLIFDPARPELTTLEATIDPRSLTLP
                     SPPAGFKDELVGPQWLNAAQYPAITFKSTKVDVTGVNTARVTGDFTLHGVTKPVVLEV
                     TFNGGYAGHPMDPNARIGFSATGVFKRSDFGIAYGVPAPGTTMGVGDEVSVVIETEFS
                     GPPLVQKPA"
     gene            complement(3039..3719)
                     /locus_tag="PLH31_02025"
     CDS             complement(3039..3719)
                     /locus_tag="PLH31_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR88055.1"
                     /translation="MTDALPLEAPAPGGELAARPRKRDAERTRKSILAAALKEFSQAG
                     FAGARIERIVKAAKCNIRMLYHYFGDKKGLYLAVLEAAYVDLRAREAELKIDFDKPLE
                     GFLELQRFTFDYFEKHPRFEGLLRNENLMHGKFAAQSRVVTETAFPLRRTIEGLIASG
                     QKQGVFGPDLDPVQIYVTIAAMSRFHLANGYSLSATLDTDMTTAEWRQARLTHALALL
                     DGWRARLG"
     gene            complement(3716..>4109)
                     /locus_tag="PLH31_02030"
     CDS             complement(3716..>4109)
                     /locus_tag="PLH31_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR88056.1"
                     /translation="RYFFILKHSGQIRHEFLFYGAMLVFAVSVIASSKPKKDVAAMSP
                     VPFSTAQAIVDRHCATCHAATPSHKGFAAAPLGAAFDTPQQIQKYASKIHERTVATTS
                     MPLGNETGMTDDERAQLGAWIQQGAKIP"
     assembly_gap    4110..4119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4120..4914)
                     /locus_tag="PLH31_02035"
     CDS             complement(<4120..4914)
                     /locus_tag="PLH31_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HQR88057.1"
                     /translation="MDYDFATWGSLVLRWLHVITGVAWIGASFYFVWLDNNLRAPAPE
                     KEGVKGELWAVHGGGFYHSQKYMTAPPHMPEHLHWFKWEAYTTWLSGFALLIVLYYVG
                     APVYLIDTAKHAFTQGQAIGVGLAFIFGGLAVYEALCRSPLGQRPRLFGIVWFAALTA
                     ATWALTQIFTDRGAFIHVGAIIGTCMVGNVFLIIIPNQRKIVADMLAGRPVDPRLGAM
                     GKQRSVHNTHMTLPVIFIMISGHYPMVTGHPLAWLLLALISAGGVSI"
     gene            complement(4914..>5265)
                     /gene="uraD"
                     /locus_tag="PLH31_02040"
     CDS             complement(4914..>5265)
                     /gene="uraD"
                     /locus_tag="PLH31_02040"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HQR88058.1"
                     /translation="DASTTADKLALIRAHPELAGKAAIAKALTAESNAEQASAGLDRL
                     TAEEFERFHALNAAYAMRFGFPFIIAVRLNDKTSILAAMQSRLANDEAQEIAEAITQI
                     GHISRLRLLDAVSA"
BASE COUNT          899 a         1628 c         1808 g          920 t
ORIGIN      
        1 aaggccgccg atctgggcgc agcaacctgg ccgcgcgccg agaccccgac ccactacatc
       61 gtcatgggct tcaacgagga tctcgacctc gccatgaagc aggccctgcg cggcatgatt
      121 aacttcatca ccgcccgcag cgccctgacg ccggtgcagg cctaccagtt ctgttcgctg
      181 gccgtggact tccgcgtcac ccagactgtc aatggtgaga agggcgtaca cggaatgctg
      241 gagaagggtg ttctattctg aaacagttct gtggattact ttgtagggcg ctcaatgttt
      301 acccttaata ttccctcacc aagtgaaacc aattctggtg tgatccgccc ctgactaaac
      361 cttcccgcgt gcccgtttgg ggcgagtgcg gggcgagcag atgttgttga acggtagcga
      421 aaaggccgac accctgacgg gcggcgccga tgcggatacg ctttacggct atggcggcaa
      481 cgacaagctg accggcggcg cgggcgacga tgtcctggcc gggatggcgg gcaacgacac
      541 gctgctgggc ggcgagggcg aagactatat cctcggcggc gcgggcgccg acacgatcga
      601 cggcggcgcg ggcgccgact gggccgccta tgaggatgcg gcggcgggcg tgaaggtcga
      661 cctgaacctg accggttcgc agaataccct gggcggcggc agcgacaggc tgatctcgat
      721 cgagaacgtc tatggctcgg ccttcaacga caccctgatc ggcgacgccg gcgtcaacta
      781 cctggccggc ggcgacggcc atgacagcct gtcgggcggc aagggcgagg acaatctgtt
      841 cggcggcgcc ggcgccgata cgctgctggg cggccagaac gacgactaca tgcagggcgg
      901 ggcgggcaac gacctgatcg acggcggcgt gggcatagac tggtcggctt atgacgacgc
      961 gacggccggc gtgacggtga acctgaccct gacggctgcg cagaacacgg gcggcggcgg
     1021 cgtcgatacg ctggtcggcg tcgaatatgt ttacggttcg gcgtttgctg atgtgctgac
     1081 gggggacgcc aacaacaaca cgctgatcgg cgatcttggc gacgacagcc tgagcggcgg
     1141 catgggtgat gacttcctgg tcggtggcgc cggcgatgac gttatcgacg gcggcgaagg
     1201 tttcgacacc atcgccctgg ttggagaccg tgacggccgg atcgatctat ccgtcgccat
     1261 tcaggacttc agcaactacg gcctcgacac tctgatctcc atcgaaggag tcatcggctc
     1321 ggacggccgg gacagtttgg cgggcaacgc cgccgagaac tatctggccg gcaagggcgg
     1381 cagtgacttt ctgtacgcgg tgggcggcgg tgacgttctg gatggcggcg agggcgacga
     1441 ctttcttaga gccagcaagg tcggctcagg tgactatatg tccggcggcg ccggcgccga
     1501 caccttcttc acgatgtggg gcgcagtcac gctggatggc ggcgacggcg ccgacctgtt
     1561 ctatgtcctg cgcgcgcccg gaatgatcgg tacgacggtc gtcaatggcg gcgccgggac
     1621 cgacacgttg gacctctcca gcggctacat accgatcggc ggcgcgatgg tcgatctctc
     1681 gatcatcggt cctcaggaca tcggcgccgg acaggttctg gatatcagcg gagtggagaa
     1741 cctcgtcggc ggccccggca atgaccttct gaaaggtgac gtcggcgcca accttatcga
     1801 gggccgggag ggagctgaca ccctgatcgg cggccaaggg cgcgatcaac tgattggcgg
     1861 cacgggaatt gacaccttcg tgttcgcggt cggcgactcc acgaccatcg acgtcacggg
     1921 ttcgggcgtg gatgtcatca gcgacttcca ggctggcgac gagctgaggt tctctgacgc
     1981 cccggccggc ttcagctatg tcgagggcag cgcgggcagc ttcgcctccg ccctggcgac
     2041 ggtgaattcc atgctgacct atcccagcac cttccacgaa tacatcgcct tccaggtcga
     2101 cgcggacgtc tatgtgttca gcggccaccc cgacgcgggt ggggcggggc tcgaaaacgt
     2161 catcctgctg gcgggcgtca atctcgacgc cgtcagctac gacagcttcc tgtagtggtc
     2221 ttgcgccgga tcgtctggag acggtccggc gccacttatt tgcgagtcgg agtctgccga
     2281 tggcggttta gaacgttcct ggcttatcga ccgacaaaaa aagcgcgaag ttcgtctagc
     2341 gccgccaagg gacacgccat gcagaagatc ctcctcgcca cggttttcct gggccttgcc
     2401 gcctgttctc ccaagccggt cgctgaaaaa gccgacgcgc cagcggccgc ccccgccgcc
     2461 aagcctgcgc cagccaatgt gccggccggc gcctataggc tcgacaagac acacgcctcg
     2521 ctggtgttta ccgtcaacca cctgggcttt tccaactaca ccgccgcctt cgccgacttc
     2581 gacgccaggc tgatcttcga tccggccagg cctgagctga caacgctgga ggcgacgatc
     2641 gatccgcgct ccctcacgct gccgtccccg ccggccgggt tcaaggacga gctggtcggg
     2701 ccgcaatggc tgaacgcggc gcaatatccg gccatcacct tcaagtcgac gaaggtggat
     2761 gtgaccggcg tcaacacggc cagggtcacc ggcgatttca ccctgcatgg cgtgaccaag
     2821 ccggtggtgc tggaggtgac cttcaatggc ggctatgccg gccatcctat ggatccgaac
     2881 gcccggatcg gcttctcggc gaccggcgtg ttcaagcggt cggacttcgg gattgcctat
     2941 ggcgtgccgg cgcccggcac gaccatgggc gttggcgacg aggtatccgt ggtcatcgag
     3001 accgagttca gcggcccgcc tctggtccag aagcccgcct agcctaaccg cgcgcgccag
     3061 ccgtcgagca gggccagggc gtgcgtcagc cgcgcctggc gccactcggc ggtggtcatg
     3121 tcggtgtcga gcgtcgccga cagcgaatag ccgttggcca ggtggaaccg gctcatcgcc
     3181 gcgatggtca catagatctg cacggggtcg agatcggggc cgaacacccc ctgtttctgg
     3241 ccgcttgcga tcagtccctc gatcgtccgg cgcagcggga aggcggtctc ggtgaccacg
     3301 cgtgactggg cggcgaactt gccgtgcatc aggttttcgt tgcgcagcag gccctcaaac
     3361 cgcgggtgct tttcgaagta gtcgaaggtg aagcgctgca gttccaggaa cccctcgagc
     3421 ggcttgtcga agtcgatctt cagctcggcc tcgcgggcgc gcaggtccac ataggcggcc
     3481 tccaggacgg ccaggtacag acccttcttg tcgccgaaat agtgatagag catccggata
     3541 ttgcacttgg cggccttgac gatccgctcg atccgggccc cggcgaaacc ggcctgcgag
     3601 aactccttca gggccgccgc caggatggat ttgcgggtgc gttcggcgtc gcgcttgcgg
     3661 ggcctggcgg cgagttcgcc gccaggggcg ggggcttcca gaggcagggc gtcggtcacg
     3721 ggatcttggc tccttgctgg atccaggcgc ccagctgggc gcgttcgtcg tcggtcatgc
     3781 ctgtctcgtt gccaagcggc atgcttgtgg tggctaccgt gcgttcgtga attttcgacg
     3841 catatttctg gatctgctgg ggcgtatcga aggcggcgcc caacggtgcg gcggcgaagc
     3901 ccttgtggct gggcgtagcg gcgtgacagg tggcgcagtg cctgtcgacg atggcctggg
     3961 cggtcgaaaa cggcacgggc gacatcgcag cgacgtcctt cttgggcttg ctgctggcga
     4021 tcacgctgac ggcgaagacc agcatggccc cgtagaacag gaactcgtgc cggatctggc
     4081 ccgagtgctt gaggatgaag aagtagcggn nnnnnnnnng atcgacaccc cgccggcgct
     4141 gatcagggcc agcagcagcc aggccagcgg gtggcccgtc accatcggat agtggccgct
     4201 gatcatgatg aagatgaccg gcagggtcat gtgggtgttg tgcaccgacc gctgcttgcc
     4261 catcgccccc aggcgcgggt ccaccgggcg gccggccagc atgtcggcga cgatcttgcg
     4321 ctggttggga atgatgatca ggaacacgtt gccgaccatg caggtgccga tgatcgcgcc
     4381 cacatggatg aaggcgcccc ggtcggtgaa gatctgggtc agggcccagg tcgccgccgt
     4441 cagggcggcg aaccagacga tcccgaacag gcgcgggcgc tggcccagcg gcgagcggca
     4501 cagggcctca tagactgcaa ggccgccgaa gatgaaggcc aggccgacgc cgatggcctg
     4561 gccttgcgtg aaggcgtgct tggcggtgtc gatcagatag accggcgcgc ccacgtaata
     4621 gagcacgatc agcagggcga agcccgacag ccaggtggtg taggcctccc acttgaacca
     4681 gtgcagatgc tcgggcatgt gcggtggggc cgtcatgtac ttctgcgagt gatagaaccc
     4741 gccgccgtgc acggcccaca gctcgccctt cacgccctcc ttttcggggg ccggcgcgcg
     4801 cagattgttg tccagccaga cgaagtagaa cgacgcgccg atccaggcga cgccggtgat
     4861 cacatgcagc cagcgcagga ccaggctgcc ccaggtggcg aaatcatagt ccatcaggcg
     4921 ctcaccgcgt cgagaaggcg gagccgggag atgtggccga tctgtgtgat cgcctcggcg
     4981 atctcctggg cttcatcgtt ggccagccgg gactgcatgg cggccaggat cgaggtcttg
     5041 tcgttcagcc gtacggcgat gatgaacgga aagccgaagc gcatcgcata ggcggcgttc
     5101 agggcgtgga agcgctcgaa ctcctcggcc gtcagcctgt cgaggccggc gctggcctgt
     5161 tcggcgttgc tctcggcggt cagggccttg gcgatggcgg ccttgccggc cagttcgggg
     5221 tgggcgcgga tcagggccag cttgtcggcg gtagtcgagg cgtcg
//
LOCUS       DAOMEO010000052         5255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2008_length_5255_cov_3.193846, whole genome shotgun sequence.
ACCESSION   DAOMEO010000052 DAOMEO010000000
VERSION     DAOMEO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_5255_cov_3.193846"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLH31_02045"
     CDS             complement(<1..732)
                     /locus_tag="PLH31_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HQR88059.1"
                     /translation="MSHLPPDPPLDGRPSYEPERKASSASVLSAPRFPVTRWNRKYLM
                     AGAAVLASIVAGGFYLGFGGAHASKGRPDDSQNAADTSSPQTPEIATRYAAGYADPAV
                     RPGTTSLPPPEALPPAGGTAPAAGQPGQPTPVDPAVQAAREQALAARAASPFFGGAQA
                     QPQAASQTPDPAPMLAAALAPGFGALPVPAAGDVQPANGQAGKRQFAAGARVDDYLTN
                     PLQAPISPWEVKAGTIISAALITAIN"
     gene            complement(736..1764)
                     /gene="trbG"
                     /locus_tag="PLH31_02050"
     CDS             complement(736..1764)
                     /gene="trbG"
                     /locus_tag="PLH31_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HQR88060.1"
                     /translation="MRDKSLRAWGVPGRMGGLVVVLAMAGGGAVLAQAAAPQSVTVRS
                     VIPAPASRSAPASAVTTTTTTSVAPVAKAAAPRRPQRRAAPRTYARPGPLRTVQSANS
                     SARDYPTSGAYINSALYYDFEPGRLYTVHASPHFLTAITLRPGEKLISKAAGDTIRWV
                     LGETVQGAGASQQVIVMVKPIRGNLRTNIILTTDQRTYLLDARSYEGDTYTSVISWNY
                     PQEEMRDAQAARAAEAQSVVASGLAIDQLHFGYDVRAIHSRKPSWQPIRVFDDGLKTY
                     IQFPTNMAATEAPPLFLIGADKQVQLVNYRYLGGYYVVDRLIDVAELRLGEKNQTVVR
                     ITRTRDRG"
     gene            complement(1770..2453)
                     /gene="trbF"
                     /locus_tag="PLH31_02055"
     CDS             complement(1770..2453)
                     /gene="trbF"
                     /locus_tag="PLH31_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HQR88061.1"
                     /translation="MNPFKRPNDRYGSSAPVESPYLRASREWDNRIGSAVVQAKNWRM
                     AAFGCMGLAALALGGFIYEASNTNIATYVVPIDKYARPGRIELAGRTYQPTTAEIGYF
                     LADWVRRTRSKSIDPIVIRDNWTGAYHFVAGPAIGQLNSYAKANDPFANAGTQAINVE
                     IVSVLARSPSTYQVQWRETQFNAGTSGVTENWTGLFTAKINPPKNEAELRANPLGIFI
                     TAFQWSREL"
     gene            complement(2474..3793)
                     /gene="trbL"
                     /locus_tag="PLH31_02060"
     CDS             complement(2474..3793)
                     /gene="trbL"
                     /locus_tag="PLH31_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HQR88062.1"
                     /translation="MATAGTSSLDEFITRFTTQIDSGFGVISGDVQVVLGTLVVISIV
                     LTAIMWAVDETQNVIAPLVRKILLVGFFAWLVGNWHSLTVTVVTGFGQLGLKAGGGAS
                     IGDFMSAPTRAVEAGFKVFLQIIQYIGELASQNGGFGALQHIDMIIVAAVAAIGILLA
                     FIILAIEVAITIIEFHLVTLIAFVTIPFGVLTQTSFLAERAIGYVVSVGLKFMALGVI
                     LGVGLNIFESYTLSPEPTVPEECGLLLAAIFLMMLALKIPAIAGALISGGPQLNSGSA
                     LMGAAGVAAGAAGVALAGRAVAGAVGSLVGGGGQVAAARAASGAINSGGSGGGGPSGG
                     GGSPSGSGPAPASPRSPASPAASAGRAAGGGVSPEGAPPPEPAPNKPSPTSSPGGASL
                     VARAEARRGDGLTSMARNTAAAATAGGDSQGPGMSAAATRRDPEPEA"
     gene            complement(3825..4052)
                     /locus_tag="PLH31_02065"
     CDS             complement(3825..4052)
                     /locus_tag="PLH31_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88063.1"
                     /translation="MVISLRTTASLAALMCLGLAGCSREAAQPTAGQKAEPVPAAGRK
                     CPDPNIRDRNDPCSPYYWKPKDSALKAAKTF"
     gene            complement(4052..4771)
                     /locus_tag="PLH31_02070"
     CDS             complement(4052..4771)
                     /locus_tag="PLH31_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HQR88064.1"
                     /translation="MRKLLSTTAAIAALAMGMSAATPPPARAQMTVIDPAAIAQMTSQ
                     VANSLRQIEQLQSQITNQVAMLQKLGTDVTQPLTQINQQATQLLQQAQAIGYNSQNIA
                     QQFQQLYPSDMTGQSFAQIRQRLASWESNSRLTMQDSMAAQNQLVRSQATTAGAVNSA
                     VAASQGAAGQTAAVQATNQLLAALSTQLQGLQTILIAQARTQDTILAQQQAGAIAARA
                     ETQRATQWTPQGSSLSKATSF"
     gene            complement(4764..5129)
                     /locus_tag="PLH31_02075"
     CDS             complement(4764..5129)
                     /locus_tag="PLH31_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88065.1"
                     /translation="MDGSIVSDADDGRDLGPEPSPLSARDIARLAWFARRLPLAWIAN
                     RCRRRLFFLALAAPGFFAVFGTILHHNFFLMLAGFVLFAAAVIFRQRTEPLFERAAAA
                     GLWPLPHSAIPPAKDTHHA"
BASE COUNT          814 a         1678 c         1919 g          844 t
ORIGIN      
        1 gttgatagcc gtgatcaggg cggccgagat gatcgtgccg gccttgacct cccaaggact
       61 gatcggcgcc tgcagggggt tggtcagata gtcgtcgacc ctggcgccgg ccgcgaactg
      121 gcgcttgccg gcctggccat tggccggctg tacatcgccg gccgccggca cgggcagcgc
      181 gccgaagcca ggcgccagcg ctgcagccag catcggcgcc ggatcgggag tctgcgaggc
      241 ggcctgcggc tgagcctggg cgccgccgaa gaacgggctg gccgcgcggg cggccagcgc
      301 ctgctcgcgc gccgcctgca cggcgggatc caccggcgtc ggttgcccgg gttggccagc
      361 ggccggcgct gtcccgccag cgggcggaag cgcttctggc ggcggcagac tcgtcgtgcc
      421 cggtcgcacc gccggatcgg cgtagccagc cgcgtagcgg gtggcgatct ccggcgtctg
      481 cgggctcgat gtgtcggcgg cgttctgcga gtcgtcgggc cgacccttgg aggcgtgcgc
      541 cccgccaaag cccaggtaga atccgccggc cacgatgctg gccaggacgg cggcgcccgc
      601 catcagatac ttgcggttcc agcgggtgac cgggaacctg ggcgcgctga gcacgctcgc
      661 cgagcttgcc ttgcgttcgg gctcgtaaga cggccgccca tcaagcggcg gatctggggg
      721 gagatggctc atcgatcagc cccgatcccg ggtgcgggtg atccgcacga ccgtctggtt
      781 cttctctcca agccgcagct cggccacgtc gatcagccgg tccacgacgt agtagccgcc
      841 caggtagcgg tagttgacca gctggacctg cttgtcggcc ccgatcagga acagcggcgg
      901 ggcctcggtc gcggccatat tggtcgggaa ctggatgtag gttttcagtc cgtcgtcgaa
      961 cacccggatg ggctgccagc taggcttgcg cgaatggatc gccctgacgt cgtagccgaa
     1021 atgcagctgg tcgatggcca gccccgaagc cacgaccgac tgggcttcag cggcgcgggc
     1081 cgcctgggcg tcgcgcatct cctcctgcgg atagttccag ctgatgacgc tggtataggt
     1141 gtcgccctcg tagctgcggg catccaggag gtaggtccgc tggtccgtgg tcaggatgat
     1201 gttggtccgc aggttcccgc gaatgggttt gaccatcacg atgacctgct gcgacgcgcc
     1261 cgcgccctgg acggtttcgc cgagcaccca tcgaatggtg tcgccggccg ccttggagat
     1321 cagcttctcg cccggccgca aggtgatggc ggtgaggaag tgcggactgg cgtggacggt
     1381 gtagagccga cccggctcga agtcgtaata gagcgccgaa ttgatgtagg cgccggaggt
     1441 cggatagtcg cgggcgctgc tgttggccga ctgcacggtg cggagcgggc ccggccgagc
     1501 gtaggtccgc ggcgccgcac gccgctgggg ccgcctgggc gccgccgcct tggcgaccgg
     1561 cgcgacgctc gtggtcgtcg tcgtggtgac cgcgctggcg ggcgcggagc gtgaggcggg
     1621 cgcggggatg accgaccgga ccgtcactga ttgcggcgcc gcagcctgcg ccaggaccgc
     1681 gccgccgccg gccatcgcca gaacgaccac caggccgccc atccgtccgg gcacgcccca
     1741 ggcgcggagg gatttatctc tcatggcgct catagctcgc gactccactg aaatgcggtg
     1801 atgaagatgc ccagcgggtt ggcgcgcagc tcagcctcgt tcttcggagg gttgatcttg
     1861 gcggtgaaga gcccggtcca gttctcggtg acgccggacg tcccggcgtt gaactgcgtc
     1921 tcgcgccact gcacctggta ggtgctggga ctgcgggcca ggaccgagac gatctccaca
     1981 ttgatcgcct gagtgccggc gttggcgaac ggatcgttgg ccttggcgta gctgttgagc
     2041 tggccgatcg ccgggccggc gacgaagtgg taggccccgg tccagttgtc ccggatgacg
     2101 atggggtcga tgcttttgga gcgggtgcgc cgcacccagt cggccaggaa gtagccgatc
     2161 tccgccgtcg tcggctggta ggtccggccc gccagctcga tgcggccggg ccgggcgtat
     2221 ttgtcgatcg gcacgacata ggtggcgatg ttggtgttgc tggcttcata gatgaagccg
     2281 cccagcgcca gcgcggcgag gcccatgcag ccgaaggcgg ccatgcgcca gttcttggcc
     2341 tggacgacgg cggagccgat ccggttgtcc cattccctgg aggcccgcag gtaggggctc
     2401 tcgaccggcg ccgagcttcc atatcgatca ttggggcgtt tgaaggggtt catctacgct
     2461 cttctcactg gggttaagcc tccggctcgg gatcgcggcg ggtcgccgcg gcggacattc
     2521 cggggccctg gctgtcgccg cccgccgtgg cggccgcggc ggtgttgcgc gccatgctgg
     2581 tcaggccatc gccgcgacgg gcttcggcgc gggccacgag ggaggcgccg ccaggggaag
     2641 acgtagggga gggcttgttc ggcgccggct ccggaggcgg cgcaccctcg ggcgaaacgc
     2701 cgccaccggc agcacgaccg gcagaggcgg ccggagaggc tggcgatctg ggggacgctg
     2761 gagccgggcc ggatcctgac ggagaaccgc cgccgcccga cgggccaccg ccacctgaac
     2821 cgccagaatt gatggcgccg gacgcggcac gggccgcagc gacttgcccg ccgccgccca
     2881 ctaggctgcc cacggctccg gcaaccgcgc gaccggccag ggccacgccg gcggcgccgg
     2941 ccgccacacc ggcggcgccc atcagcgcgc tgcccgagtt gagctgcggg ccacccgaga
     3001 tcagcgcgcc ggcgatggcc ggaatcttca gcgccagcat catcaggaag atcgcggcca
     3061 gcagcagccc gcattcctcc gggaccgtgg gctcgggaga cagggtgtaa ctttcgaaga
     3121 tgttcaggcc gacgccgaga atgacgccga gcgccatgaa cttcagaccg accgagacga
     3181 cgtagccgat cgcccgctcg gcgagaaacg aggtctgtgt cagcaccccg aaggggatcg
     3241 tcacgaacgc gatgagcgtc acaaggtgga actcgatgat cgtgatcgcg acctcgatcg
     3301 ccaggatgat gaaggcgagc aggatcccga tcgccgccac cgctgcgacg atgatcatgt
     3361 cgatatgctg cagcgcgccg aagccgccgt tctgggaggc aagctccccg atgtactgga
     3421 tgatctgcag gaagactttg aagccggctt ccacggcgcg ggtcggcgcg ctcatgaagt
     3481 cgccgatgct ggcgccgccg cccgctttga gaccgagctg accaaagccg gtgacgacgg
     3541 tcaccgtcag ggagtgccag ttccccacca gccaggcgaa gaagcccacc aacaggattt
     3601 ttcggacgag cggggcgatg acgttctggg tttcatcgac agcccacatg atggcggtga
     3661 gcacgatgct gatgacgacg agcgtgccga gaacaacctg aacatcgccc gaaatgacgc
     3721 cgaagccgct gtcgatctgg gtcgtgaacc gagtgatgaa ctcgtcgagc gatgatgtgc
     3781 cggctgtcgc cattactaaa atgtccttgg atatttcccc tcgttcagaa ggtcttggcg
     3841 gccttcaggg cgctgtcttt tggcttccag tagtagggag agcagggatc gtttcggtcg
     3901 cggatgttgg ggtccggaca cttgcggccg gccgccggga ccggctcggc cttctgaccg
     3961 gccgttggct gcgcggcctc tcggctgcaa ccagcaaggc cgaggcacat cagagcggcg
     4021 agcgaggccg tggttctcag agatatgacc atcagaaact cgttgccttg gagaggctgc
     4081 tgccctgcgg cgtccattgg gtggcgcgtt gggtttcggc gcgagcggcg atggcgccgg
     4141 cctgctgttg ggccaggata gtgtcctggg tgcgcgcctg agcgatgagg atggtctgca
     4201 ggccctgcag ctgagtgctc aaggccgcca gaagctggtt ggtggcctga acggccgccg
     4261 tctggccagc ggcgccctgc gacgcggcga ccgcgctgtt cacggcgccg gcagtcgtcg
     4321 cctgcgaccg gaccagttgg ttctgggccg ccatcgaatc ctgcatggtc agccggctgt
     4381 tgctctccca gctggccaac ctctgacgga tctgagcgaa gctctggccg gtcatgtccg
     4441 acgggtagag ctgctggaac tgctgggcga tgttctggct gttgtagccg atggcctggg
     4501 cctgctggag cagctgggtg gcctgctgat tgatctgcgt cagcggctgg gtgacgtcgg
     4561 tccccagctt ctgcagcatc gccacctggt tggtgatctg cgactggagc tgctcgatct
     4621 ggcgcaggct gttggcgacc tgggaggtca tctgggcgat ggcggccgga tcgatgaccg
     4681 tcatctgcgc gcgcgccggc gggggcgtcg cggcggacat ccccatcgcc aaggccgcga
     4741 tcgcggccgt cgtcgacaac aacttacgca tggtgagtgt ccttcgctgg ggggatggca
     4801 gagtggggga ggggccagag gcccgccgcg gccgcccgct caaacagggg ttcggtccgc
     4861 tgccggaaga tcaccgcggc ggcaaagaga acgaagccgg ccagcatcag gaagaagttg
     4921 tggtggagga tcgtgccaaa gacggcgaag aaccccggag cggcgagcgc caggaaaaac
     4981 agccgacgac ggcaacggtt ggcgatccac gccaggggaa ggcgccgggc aaaccaggcg
     5041 agccgcgcga tatcgcgcgc gctaagcggc gagggctccg gcccaagatc gcggccgtcg
     5101 tcagcatccg agacgatcga accatccatc acgccacctt cggatgggcg gcgtcacgct
     5161 gggcggcaag gtagctggcc acggccgcct ggcccttggc ggcgaagaac cgttcgcaga
     5221 attgcgcggc gccgtgctcg gccagcagcc gggtc
//
LOCUS       DAOMEO010000053         5244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2015_length_5244_cov_2.556755, whole genome shotgun sequence.
ACCESSION   DAOMEO010000053 DAOMEO010000000
VERSION     DAOMEO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2015_length_5244_cov_2.556755"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLH31_02080"
     CDS             <1..474
                     /locus_tag="PLH31_02080"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQR88066.1"
                     /translation="MSTNGCGEGFEDWWGALQGETQRGERIGFAIRRLSDGKVVGTSS
                     YLNIRKLHKGLEVGSTFLHPDARSGPVNPESKRLLLANAFNAGAIRVEFLVDVRNGRS
                     QAAVEKLGAHKDGVLRNHKITWTGHVRDTAVFSITDADWPGVKQRLDFRLTESFV"
     gene            579..>1845
                     /locus_tag="PLH31_02085"
     CDS             579..>1845
                     /locus_tag="PLH31_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88067.1"
                     /translation="MTAIACLRLLIDDDAFPAARAQAAADVYLKERWKAPRRYGAVAP
                     LAFVLADNRTGQLDPTEMQALAAELESALFPGRKSGQVCLMTFEGDESAVLKFASLTQ
                     AELAGLVAGKGYGGAAGRVQVITADAIRAVPSTHEEARLAAAAASAPAPAPAPLVTKA
                     PEPAPSPALVPAEKRAAAPAPAPVAKVKAPPPPPPPPPPSQTGWWGLYDLPTEAFVGS
                     IIGLRADLTQPPPEADSALLTRDLTGLNDAQTAFKTAPFGEFHVPFGFWNLTTPHAQD
                     AYKGRLSRYPLDLQPRLGATVYGTPREASLGLVSQIRAFLKGSFAFLDLNVVDPTFPV
                     HNLPPELIDSVTLTLTGEDERERLAQILKFVERQGVYRGKGVAQIVAGVSSANELEAC
                     RQGGVARVSGPAVTAFLDKPVAQAEGAPLV"
     assembly_gap    1846..1855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1961..3193
                     /locus_tag="PLH31_02090"
     CDS             1961..3193
                     /locus_tag="PLH31_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88068.1"
                     /translation="MTALACLRIFVGADLCFTDTDSMAALVAKVAGAYLETTWKWPRR
                     YGLVAPFSFVLADPRAERLDARELQDLARALQHKLFGDQGDGDVTLLTFEGDQTDVMR
                     FAGATALQLKGLLDGEDDGAFAGRVCKITPTDVSSVAPPGGPVAGEPPMEALRAQEHM
                     VAPARTAYRGVFHTQREVFVGNISVWREAGSVSVFGMEPPAADASEAGHDIPNLESAR
                     RALEGETSGVMFFPISFSTLVKPSARAELTPYLETLPQASRARLAAAVYDTPRAPSFS
                     ALSQMKQYLNPFFGRIDLRVSDPAFQVDDLPPDLASSVTLMLPTGVEAVRLAAISRFM
                     RDAAGYRRKRIWQGVTDVRNRREVNACIELGVPFITGPGVTDLLETPAHVSPCSALHL
                     PLHDWSARVTGAAASQVA"
     gene            complement(3194..5098)
                     /gene="mutL"
                     /locus_tag="PLH31_02095"
     CDS             complement(3194..5098)
                     /gene="mutL"
                     /locus_tag="PLH31_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQR88069.1"
                     /translation="MPIRRLPPETVNRIAAGEVVERPASAIKELVDNAVDAGATRIEV
                     EAHGGGLTRILVADDGMGLSAEELPVAIERHATSKLAPDADGLWDLLRIHTMGFRGEA
                     LPSIGSVARLSISSRAKGAKDAFSILVEGGQVGDVAPAAFPGPHGARIEVRDLFYATP
                     ARLKFMKSERAEALAITEEIKRQAMAHEAVGFSLDIDGRRVLRLLPEHPGPQGRLARL
                     AAVLGRDFQDNAIEIDQTRDGVRLTGFAGLPTYNRGNAAHQYLFVNGRPVRDRLLQGA
                     LRAAYADFLARDRHPTAALYVSLDTAEVDVNVHPAKAEVRFRDPALVRGLIVGALRHA
                     LAGAGHRASTTVATQALDSIRAQGAGLGSSPFQFQAGPSPAGFSAWREGGWAPPRPQV
                     LPGLSDVSARVEPGFSDVLGQAYDAVQNTEASPSYPQAGDYAAPPFDPVDFPLGAARA
                     QVHETYIVAQTRDGMVIVDQHAAHERLVYERMKGEMAAGGVARQTLLLPEVVDLDPAE
                     AERVLARAEELATLGLIVESFGPGAVLVRETPALLGKTDAAGLIRDIADDLAENGQAL
                     ALKERLEEVCSTMACHGSVRAGRRLNGAEMNALLREMEATPHSGQCNHGRPTYVELKL
                     ADIERLFGRR"
BASE COUNT          775 a         1831 c         1786 g          842 t
ORIGIN      
        1 atgtcgacca atggctgcgg cgagggtttc gaggactggt ggggcgcgct gcagggcgag
       61 acccagcgtg gcgagcggat cggcttcgcc atccgccggc tgtcggacgg caaggtggtg
      121 ggcacctcca gctatctgaa cattcgcaag ctgcacaagg gtctggaggt gggctccacc
      181 ttcctgcatc ccgatgcgcg ctcgggtccg gtcaatccgg aatccaagcg attgctgctg
      241 gccaacgctt tcaatgccgg tgcgatccgg gtcgagtttc tggtcgacgt ccgcaacggc
      301 cgcagccagg cggcggtgga aaagctgggc gcccacaagg atggcgtgct gcgcaatcac
      361 aagattacct ggaccggcca tgtgcgcgac accgccgtgt tctcgatcac cgacgccgac
      421 tggccgggcg tcaagcagcg actggatttc cgcctgacgg agtcgttcgt ctagagggcg
      481 acccgcgcgc aattgtcgca atccccgcaa caaagataac gccgttccac cattacgtgt
      541 aacgaattgt cgagcattcg accctagcgt cgcggaccat gaccgcgatt gcttgtctcc
      601 ggctgttgat cgatgacgac gcctttcccg ccgcgcgggc ccaggcggcg gcggacgtct
      661 atctgaagga acgctggaag gcgccgcgtc gctatggcgc cgttgcgccc ctggctttcg
      721 tcttggccga caatcgcacc gggcagcttg atccgaccga gatgcaggcc ctggcggccg
      781 aactggaatc ggcgctgttc ccgggccgca agagcggtca ggtctgcctg atgaccttcg
      841 agggcgacga gagcgcggtg ctcaagttcg ccagcctgac ccaggccgag ctggccggtc
      901 tggtcgccgg caagggctat ggcggcgccg ctggccgggt tcaggtgatc accgccgacg
      961 ccattcgcgc cgtgcccagc acgcacgagg aagcgcgtct ggccgccgcc gcggcctcgg
     1021 cgccggcccc cgcgccagcg ccgcttgtga ccaaggctcc cgagcctgcg ccctcgcccg
     1081 cgcttgttcc ggccgagaaa cgcgccgccg ccccggcgcc cgcaccggtc gccaaggtca
     1141 aggcgccgcc gccgccaccg cctccgccac cgccgtccca gacgggctgg tgggggctct
     1201 atgatttacc gaccgaggcc ttcgtcggct cgattatcgg cctgcgcgcc gacctcaccc
     1261 agccgccgcc ggaagccgac tcggcgctgc tgacccgcga tctcacgggc ctcaacgacg
     1321 cccagacggc cttcaagact gcgccgtttg gcgagtttca tgtgccgttc ggcttttgga
     1381 acctgaccac gccccacgcc caggacgcct ataagggccg actgtcgcgc tatccgctgg
     1441 acctgcagcc tcgtctgggc gctaccgtct atggcacgcc gcgcgaggcc tcgctgggcc
     1501 tggtcagcca gatccgcgcc ttcctgaagg gcagcttcgc cttcctcgat ctcaacgtgg
     1561 tggaccccac cttcccggtc cacaacctgc cgcccgagct gatcgacagc gtcaccctga
     1621 ccctgaccgg tgaagacgaa cgcgagcggc tggcgcagat tctgaagttc gtcgaacgcc
     1681 agggcgtcta tcgcggcaag ggcgtggccc agatcgtggc cggcgtctcc agcgccaacg
     1741 agctggaagc ctgccgccag ggcggcgtcg cgcgggtgtc gggccctgcc gtgaccgcct
     1801 tcctcgacaa gccggtcgcc caggccgaag gcgcgccgct ggtccnnnnn nnnnnggccc
     1861 gccccaatgc tcaagctatg attgcgccgc gtcggttggt cgcgggttag atacgtctct
     1921 tcgttcaggt tcgataacct tccccggtca cgatgggcgc atgacggctt tagcgtgttt
     1981 gagaatcttc gtcggcgcgg acctgtgctt taccgatacc gactccatgg ccgcgctggt
     2041 ggccaaggtg gccggcgcct atctggaaac cacctggaag tggccgcgcc gctatggcct
     2101 ggtcgcgccg ttctcgttcg tcctggccga tccgcgcgcc gagcggctgg atgcgcgcga
     2161 gcttcaggac ctggcgcggg ccctgcagca caagctgttt ggcgaccagg gcgacggcga
     2221 tgtcaccctg ctgaccttcg agggcgacca gaccgacgtc atgcgtttcg ccggcgccac
     2281 ggccttgcag ctcaagggtc tgcttgacgg cgaagatgac ggcgccttcg cggggcgggt
     2341 ctgcaagatc accccgactg atgtctcctc cgtcgcgcct ccgggcggac ccgtggcggg
     2401 cgagccgccc atggaggccc tgcgcgcgca ggagcacatg gtcgccccgg cccgcaccgc
     2461 ctatcgcggc gtcttccaca cgcagcgcga agtgtttgtc ggcaatatct cggtctggcg
     2521 cgaagccgga tcggtctcgg tcttcggcat ggagcccccg gccgctgacg ccagcgaggc
     2581 gggtcacgac atacccaatc tggaaagcgc ccgccgggcc ctggaaggcg aaacgtccgg
     2641 ggtgatgttc ttcccgatca gtttttccac cctggtcaag cccagcgcgc gggccgaatt
     2701 gacgccctat ctggaaaccc tgcctcaggc ttcgcgggcc cggctggcgg cggcggtcta
     2761 tgacacgccc cgcgcgccgt cgttctcggc cctgtcgcag atgaagcagt atctgaatcc
     2821 gttctttggc cgcatagacc tgcgggtctc cgatccggcc tttcaggtcg atgacctgcc
     2881 gccggacctc gcctccagtg tcaccctgat gctgccgacc ggggtggagg ccgtgcgcct
     2941 ggcggccatt tcccgtttca tgcgcgatgc ggccggttat cgtcgcaagc gcatctggca
     3001 gggggtgacc gatgtgcgta accggcgcga agtgaacgcc tgcatcgaac tgggcgtgcc
     3061 gttcatcacc ggcccggggg tcaccgatct gctcgaaacg cctgcgcatg tgtcgccatg
     3121 ctcggccctg catctgccgc tgcatgactg gtccgcccgc gttaccgggg cggcggcctc
     3181 gcaggtcgcc tgactaccgc cggccgaaca atcgctcgat atcggccagt ttcagctcca
     3241 cataggtggg acggccgtgg ttgcactggc ccgaatgggg cgtggcttcc atctcgcgca
     3301 gcagggcgtt catctcggcg ccgttgaggc ggcggccggc gcggacgctg ccgtggcagg
     3361 ccatggtgga gcacacctct tccagccgct ccttcagcgc cagggcctga ccgttctcgg
     3421 ccagatcgtc ggcgatgtcg cggatcaggc cggcggcgtc ggtcttgccc agcagggcgg
     3481 gcgtttcgcg caccagcacc gcgccggggc cgaagctctc gacgatcagg cccagggtcg
     3541 ccagttcttc ggcccgggcc aggacacgct cggcctcggc agggtccaga tccaccacct
     3601 caggcagcag cagggtctga cgggcgacgc cgccggccgc catctcgccc ttcatccgct
     3661 catagacgag gcgctcgtgg gcggcgtgct ggtcgacgat gaccatgccg tcgcgggtct
     3721 gggccacgat ataggtctcg tgcacctggg cccgcgccgc gcccagcggg aagtcgaccg
     3781 ggtcgaacgg cggggcggca tagtcgccag cctgtggata actcggcgag gcctcggtgt
     3841 tctggaccgc gtcataggcc tgtcccaaca cgtcgctgaa gccaggctcg acccgcgccg
     3901 aaacgtccga gaggcccggc agcacctgcg gacggggcgg cgcccagccg ccttcgcgcc
     3961 aggccgagaa gccagcgggt gaggggccgg cctggaattg aaacggcgaa ctgcccaagc
     4021 ccgccccttg cgcgcgaatg ctgtccagcg cctgggtcgc cacggtggtc gaggcccggt
     4081 gcccggcgcc ggccagggca tggcgcagcg ctccaacgat caggccgcgc accagggccg
     4141 gatcgcgaaa ccgcacctcg gccttggcgg ggtggacgtt gacgtcgacc tcggcggtgt
     4201 cgagcgacac atagagcgcc gccgtcgggt ggcggtcgcg ggccaggaaa tcggcatagg
     4261 cggcgcgcag ggcgccctgc agcagccggt cgcgcacggg gcggccgttg acgaacagat
     4321 actgatgggc ggcgttgccg cgattgtagg tcggaaggcc cgcgaacccc gtcaggcgga
     4381 cgccgtcgcg ggtctggtcg atctcgatgg cattgtcctg gaagtcgcgc cccagaacgg
     4441 cggcgaggcg cgccagccgg ccctgcggcc cgggatgttc gggcaacagg cgcaacaccc
     4501 ggcggccgtc gatatccagc gagaagccga ccgcctcgtg ggccatggcc tggcgcttga
     4561 tctcctcggt gatcgccagg gcctcggccc gttccgactt catgaacttc agccgggcgg
     4621 gggtggcgta gaacaggtcg cgaacctcga tccgcgcccc gtgcgggccc gggaaggcgg
     4681 cgggggccac atcgccgacc tgaccaccct cgaccaggat cgagaaggcg tccttggcgc
     4741 ccttggcccg cgagctgatc gacagccgcg ccaccgagcc gatcgagggc agggcctcgc
     4801 cccggaagcc catggtgtgg atgcgcagca ggtcccacag gccgtcggcg tcgggggcca
     4861 gtttcgaggt ggcatggcgc tcgatggcaa cgggcagttc ctcggcgctc aggcccatgc
     4921 cgtcatcggc caccaggatg cgggtcaggc cgccgccatg ggcttccacc tcgatccgcg
     4981 tcgcgccggc gtcgacggcg ttgtcgacca gctccttgat ggcgctggcc ggccgttcca
     5041 ccacctcgcc ggcggcgatg cggttgacgg tctcgggcgg caggcggcgg atcggcatgg
     5101 tcgaactttc ggaggcaggc gcacgctggc ccgccgggac gcagagaaac ctcacactag
     5161 agcgattccg ccagcccatg gcgcaaggac atcggatgga aagcccgcat cttgcggcca
     5221 cgctttcgcc gcagcccggc tatc
//
LOCUS       DAOMEO010000054         5231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2023_length_5231_cov_2.610124, whole genome shotgun sequence.
ACCESSION   DAOMEO010000054 DAOMEO010000000
VERSION     DAOMEO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2023_length_5231_cov_2.610124"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLH31_02100"
     CDS             <1..538
                     /locus_tag="PLH31_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQR88070.1"
                     /translation="IGDMPPALIAAGATLVLNRGGERRQMPLEAFFLDYGRQDRRPGE
                     FVEAVIAPTPRPGLVFKVFKLTKRFDQDISAVCGAFALTIEGGVVASARIAFGGMAGT
                     PKRAGACEAALIGQPWSEATVEAAMAALDIDYTPMSDMRASAAYRSLTARNMLRKVFL
                     ESSSPGLLARVEPESAHG"
     gene            561..2870
                     /gene="xdhB"
                     /locus_tag="PLH31_02105"
     CDS             561..2870
                     /gene="xdhB"
                     /locus_tag="PLH31_02105"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HQR88071.1"
                     /translation="MTDAFQGVHASLPHDSAARHVAGSAVYIDDLPEPAGMLHVHFGL
                     STRAHARITAMDLSAVRVAPGVVLVLTAADIPGENDVSPVIHDDKLFATDTVTYVGQS
                     LFAVAATSIAAARAAAAKAVIAYAELPAAIDISAAREADLKIENSQRMARGDAAAALA
                     ESPRRLQGQFAIGGQDHFYLEGQIALAIPGEDGDVHVWSSTQHPSEVQHLVARVLDRP
                     HTSVTIEVRRMGGGFGGKETQASLFAAAAALVAVKTGRAAKCRPDRDEDMIMTGKRHD
                     FEVGYDVGFDDDGRLTGIALELASRCGATTDLSMAINDRAMFHADNCYFLPAVEILSH
                     RFRTHTVSNTAFRGFGGPQGMIAIERVMDAVAQATGLDPLEVRQRNLYGEPGRDLTPY
                     HQTVEDNVAPQLLAELAASCDYEARQREIDAFNAASPILKKGLALTPVKFGISFTTTH
                     LNQAGALIHLYADGSILLNHGGTEMGQGLFTKVAQIVAEAFQVDVSLVKATATVTDKV
                     PNTSATAASSGTDLNGMAALNAANTLKARLVEFAAKKWGCAEAEVAFTPRGLKAGAEL
                     IPFNDLCRQAHLGRVSLSSTGFYATPKIHYDRASHSGRPFYYFAYGAACSEVIIDTLT
                     GEMKVTRADILHDVGKSLNPAIDLGQIEGGFIQGMGWLTTEELVFDDQGRLRTHAPST
                     YKIPTIGDRPAHLAVKLWEAGRNVEATVHRSKAVGEPPLMLAISVFSAITRAVASVAD
                     HRIMPNLDAPATPERILMAVEDVKARARV"
     gene            3030..4037
                     /gene="xdhC"
                     /locus_tag="PLH31_02110"
     CDS             3030..4037
                     /gene="xdhC"
                     /locus_tag="PLH31_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HQR88072.1"
                     /translation="MNWARSALARISEDQPAALVTVLATEGSAPREAGTKMVVWQGGQ
                     SGTIGGGNLEHQAADQARKMLTGSQASFAIQDYPLGPLLAQCCGGRVRLLIERIEAAS
                     RDWLTDAARRMDAAQPFEVRTRFEPGLLVKTIVPIAALSDAPPVSLSGAAASARGARP
                     ELGDELVERNDAPRPPLLLFGAGHVGQAIARAFAPLPFRLFWYDSRPETAEIPGVTVA
                     EPAEMAAAAMGATREAYSLILTHDHGLDYALTSAGLAGCAGYLGLIGSKTKRARFMSR
                     LRDDGFSEAALTRLTCPIGLPHLKSKAPEVIAVSVAADLLMRLEAARISNDEGAAVAS
                     L"
     gene            4024..>5231
                     /gene="guaD"
                     /locus_tag="PLH31_02115"
     CDS             4024..>5231
                     /gene="guaD"
                     /locus_tag="PLH31_02115"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HQR88073.1"
                     /translation="MQAYRASILHLLDDPTKTEEAVAFHEDGLLLVEDGHVVACGDYA
                     SLHELLGDAPVEDLSGKLITSGFIDTHIHFPQVDIIAAHGAQLLDWLEQHTFPAEAAF
                     ADPEHAAEAATFFVDELLRNGTTTALVFGSVHKGSVEALFRAALARDMRLIAGKALMD
                     RNAPDGLTDTVETSRADMESLIADWHGKGRLGYAVTPRFAISCSDAQLAMAGEVLAQH
                     PGVWMQTHLSENPREIDETAALFPEARDYLDVYDRFGLLGQRSVFAHCVHLQGEAFGR
                     LAAKGGAVAFCPTSNLFLGSGLFPLETACAHGVKVGIGTDVGAGTSFSILHTLGEAYK
                     VGQLRGAALDPFHALYLATLGGARALDLGDRIGNLAPGKEADFLVLDLAATPLLARRL
                     AGDRSLENRL"
BASE COUNT          798 a         1810 c         1788 g          835 t
ORIGIN      
        1 gatcggcgat atgccgcccg ccctgatcgc agcgggggcg actctggttc tgaaccgggg
       61 cggtgagcgg cgccagatgc cgctggaggc cttctttctc gactatggcc ggcaggaccg
      121 gcggcccggc gagttcgttg aggcggtgat cgcaccgacg ccaagacccg ggcttgtctt
      181 caaggttttc aagctgacca agcgtttcga ccaggatatc tcggccgtct gcggggcctt
      241 cgccctgacg atcgaaggcg gtgtcgtggc atcggcgcgg atcgcgttcg gcggcatggc
      301 gggcacgccc aagcgggcgg gagcctgcga ggcggccctg atcggccagc cgtggagcga
      361 ggcgacggtt gaagcggcca tggcggcgct cgatatcgac tatacgccga tgagcgacat
      421 gcgcgcctcg gcggcctatc ggtcgctcac cgcccgcaac atgctgcgca aggtgttcct
      481 cgagtccagt tcgcctggcc tcttggctcg cgttgaaccg gaaagcgccc atggctgact
      541 ccgccgcgac agcgcctccg atgaccgacg cgttccaggg cgtgcatgcc tcgctgccgc
      601 atgacagcgc cgcgcgccat gtcgcgggct cggccgtcta tatcgacgac ctgcccgagc
      661 cggcggggat gctgcacgtg catttcggcc tcagcaccag ggctcatgcg cgcatcaccg
      721 cgatggacct gtcggccgtc cgcgtcgcgc ccggtgtggt gctggtgctg accgccgccg
      781 acatccccgg cgagaacgac gtcagtccgg tgatccacga cgacaagtta ttcgccaccg
      841 acaccgtgac ctatgtcggc cagagcctgt tcgcggtggc cgccacctcg atcgccgccg
      901 cccgcgccgc cgccgccaag gcggtgatcg cctatgccga gctgccggcc gccatcgata
      961 tttccgccgc gcgtgaggcc gatctgaaga tcgagaacag ccagcggatg gcgcgaggcg
     1021 acgcggccgc cgcgctggcg gaatcgcccc gacgtctgca gggccagttc gccatcgggg
     1081 gacaggacca cttctatctc gaggggcaaa tcgccctggc catcccgggc gaggacggcg
     1141 acgtccacgt ctggtcgtcc acccagcatc ccagcgaggt acagcatctg gtggcccgcg
     1201 tgctggaccg gccgcacact tcggtgacca tcgaggtgcg ccggatgggc ggcgggttcg
     1261 ggggcaagga aacccaggcc tcgctgttcg ccgccgccgc cgccctggtg gcggtgaaga
     1321 ccggccgcgc cgccaaatgc cggcccgacc gcgacgaaga catgatcatg accggcaagc
     1381 ggcatgactt cgaggtcggc tatgatgtcg gctttgacga cgacggccgc ctgaccggca
     1441 tcgctctgga actggcctcg cgctgcgggg cgaccaccga cctgtcgatg gcgatcaacg
     1501 accgggcgat gttccacgcc gacaactgct atttcctgcc ggcggtcgag atcctgtcgc
     1561 accgcttccg cacccatacg gtgtcgaaca cggcgttccg aggctttggc ggaccgcagg
     1621 gcatgatcgc catcgagcgg gtgatggacg ccgtggccca ggccaccggt cttgatccgc
     1681 tggaggtgcg tcagcgcaat ctatacggcg agcctggccg cgacctgacg ccctatcacc
     1741 agaccgtcga ggacaatgtc gcgccgcagc tgctggccga gctggccgcc tcctgcgact
     1801 acgaggcccg tcagcgcgag atcgacgcgt tcaacgccgc cagcccgatc ctgaaaaagg
     1861 gcctggccct gacgccggtg aagttcggca tctcgttcac gaccacgcac ctgaaccagg
     1921 ccggcgccct gatccacctc tatgccgacg gctcgatcct gctgaaccat ggcgggacgg
     1981 agatggggca gggcctgttc accaaggtcg cccagatcgt cgccgaggct ttccaggtgg
     2041 atgtgtcgct ggtgaaagcc actgctaccg tcaccgacaa ggtgcccaac acttcggcca
     2101 cggccgcctc gtcgggcacc gacctgaacg gcatggcggc gctcaatgcg gccaacaccc
     2161 tcaaggcccg gctggtcgag ttcgccgcaa agaagtgggg ctgcgctgag gccgaggtcg
     2221 ccttcacgcc ccggggcctg aaggccggcg ccgagctgat cccgttcaac gatctgtgcc
     2281 gtcaagccca tctgggccgg gtgtcgctgt cctcgaccgg cttctacgcc acgcccaaga
     2341 ttcactacga ccgggccagc cacagcggcc ggccgttcta ctacttcgcc tatggcgcgg
     2401 cctgcagcga ggtgatcatc gacaccctga ccggcgagat gaaggtgacc cgcgccgaca
     2461 tcctgcacga cgtgggcaaa tccctgaacc cggccatcga cctgggtcag atcgagggcg
     2521 gcttcatcca gggcatgggc tggctgacca ccgaagagct ggtgttcgac gatcagggcc
     2581 gcctgcgcac ccacgcgcct tcgacctaca agatcccgac catcggtgac cgtccggccc
     2641 atctggcggt caagctctgg gaggccggcc gtaatgtcga ggccaccgtt caccgctcca
     2701 aggccgtggg cgagccgccg ctgatgctgg cgatctcggt gttcagcgcc atcacccggg
     2761 cggtggctag cgtggcggat catcggatca tgccgaacct ggatgcgccg gcgacgccgg
     2821 agcggatcct gatggcggtc gaggatgtga aggcgagggc ccgtgtttaa gtccctctcc
     2881 cagagggaga gggtggggcc cggcgcgaag cgctgggagg gtgaggggtt acgccctcaa
     2941 ccggcgtgcc ggcacgccgt catgctcctt aacccctcac cctcccatgc tgcgcatggg
     3001 cccctccctc tccctctggg agagggttta tgaattgggc tcgttccgcg ctggcccgga
     3061 tcagcgaaga ccaacccgcc gccctggtca ccgttctggc caccgaaggc tcggcgccgc
     3121 gcgaggccgg gaccaagatg gtggtctggc agggcggcca gtcgggcacc atcggcggcg
     3181 gcaatctgga gcatcaggcc gccgaccagg cccgcaaaat gctgaccggc tcgcaggcca
     3241 gcttcgccat ccaggactat ccgctgggtc cgctgctggc ccagtgctgc ggcggccgcg
     3301 tgcggctgct gatcgagcgg atcgaggccg ccagccgcga ctggctgacc gacgccgccc
     3361 ggcggatgga cgccgcccag ccgttcgagg tgcgcacccg ctttgagccg ggcctgctgg
     3421 tcaagaccat tgtcccgatc gccgccctgt cagatgcgcc gccggtcagc ctgtccggcg
     3481 ccgccgcctc ggcgcgcggg gcgcggccgg agctgggcga cgagctggtc gagcgcaatg
     3541 acgcgccgcg tccgccgctg ctgctgttcg gcgccggcca tgtgggtcag gccatcgcga
     3601 gggccttcgc gccgttgccg ttccgactgt tctggtacga cagccggccc gagacggctg
     3661 aaattcccgg ggtcactgtg gctgagccgg ccgagatggc cgccgccgcc atgggcgcga
     3721 cgcgggaagc ctacagcctg atcctaaccc acgaccacgg cctggattac gccctgacct
     3781 cggccggcct ggccggctgc gccggctatc tgggcctgat cggctcgaag accaagcgcg
     3841 cccggttcat gagccggctg cgcgacgacg gcttctcgga ggcggcgctc acccgcctga
     3901 cctgcccgat cggcctgccg cacctaaaga gcaaggcccc cgaggtgatc gccgtctcgg
     3961 tggccgccga cctgctgatg cgcctggagg cggcgcggat ttccaatgac gagggcgccg
     4021 ccgttgcaag cctatagggc gtcgatcctc cacctgctgg acgatccgac gaagaccgag
     4081 gaggccgtgg ccttccacga ggacggcctg ctgctcgtgg aggatggcca tgtcgtggcc
     4141 tgtggtgact acgcgtcgct gcatgagctg ctgggcgacg cgccggtcga ggacctgagc
     4201 ggcaagctga tcacctccgg cttcatcgac acccacatcc acttcccgca ggtcgatatc
     4261 atcgccgccc atggcgcgca gctgctggac tggctggagc agcacacctt cccggcggag
     4321 gcggcgttcg ccgatccgga acacgccgcc gaggccgcga ctttctttgt cgacgagctg
     4381 ctgcgcaacg gtacgaccac ggcactggtg tttggctcgg tgcacaaggg ctcggtcgag
     4441 gccctgttcc gcgcggcgct ggcgcgcgac atgcgcctga tcgccggcaa ggcgctgatg
     4501 gaccgcaacg cgcccgacgg cctgaccgac acggtcgaga ccagccgcgc tgacatggaa
     4561 agcctgatcg ccgactggca tggcaagggg cgcctgggct acgccgtcac cccgcgcttt
     4621 gcgatcagct gcagcgacgc gcaactggcc atggccggcg aggtgctggc ccagcacccc
     4681 ggcgtctgga tgcagaccca cctgtcggag aacccgcgcg agatcgacga gacggcggcg
     4741 ttgttccctg aggcccgcga ctatctggac gtctatgacc gctttggcct gctgggccaa
     4801 cgctcggtct tcgcccactg cgtccacctg cagggcgagg cgttcgggcg gctggcggcc
     4861 aagggcgggg cggtggcttt ttgcccgaca tcgaacctgt tcctgggttc aggcctgttc
     4921 ccgctggaga ccgcctgcgc ccatggggtg aaggtgggca tcggcaccga tgtcggggcg
     4981 ggaacgagct tctcgatcct tcataccctg ggcgaggctt acaaggtcgg ccagctgcgc
     5041 ggcgcggcgc tggatccgtt ccatgcgctg tatctggcca ccctgggcgg tgcgcgggcg
     5101 ctggatttgg gcgacaggat cggcaatctg gcgcccggca aggaggctga cttcctggtg
     5161 ctggatctgg ccgccacgcc gctgctggcg cggcgtctgg cgggcgatcg cagtctggag
     5221 aaccgattgt t
//
LOCUS       DAOMEO010000055         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2034_length_5202_cov_2.769769, whole genome shotgun sequence.
ACCESSION   DAOMEO010000055 DAOMEO010000000
VERSION     DAOMEO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_5202_cov_2.769769"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLH31_02120"
     CDS             <1..739
                     /locus_tag="PLH31_02120"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HQR88074.1"
                     /translation="DAMEACIDRGMRQDGVLPGGLDVKRRARQIHQTIQGRMERQISD
                     PLAAMDFVNLWAMAVNEENAAGGRVVTAPTNGAAGLVPAVLRFFVRFYNGTPEQIRVF
                     LLTAAAIGALYKRNASISGAEVGCQGEVGVACSMAAAGLCAALGGTNAQIENAAEIGM
                     EHNLGLTCDPIGGLVQIPCIERNAMGAIKAIDAARLALLGDGQHSVSLDKVIATMKRT
                     GEDMNEIYKETSMGGLAVGLSVNRVEC"
     gene            771..923
                     /locus_tag="PLH31_02125"
     CDS             771..923
                     /locus_tag="PLH31_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88075.1"
                     /translation="MLLALAAVPVARCTREYFTVDACLDSGGAWIEQIDKCSHDQAEL
                     DRYKPR"
     gene            1259..2014
                     /locus_tag="PLH31_02130"
     CDS             1259..2014
                     /locus_tag="PLH31_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HQR88076.1"
                     /translation="MSNGVIRTGIGGWTFEPWRGVFFPDTVKQKDELKYASSQLTSIE
                     INGTYYSTFKPNSWMKWRDETPDDFVFAVKASRYCTNRKVLSENNDSLEKFLTQGLEE
                     LGDKLGPINWQFMATKKFDPVDFEGFLKLLPREIGGRPARHALEVRSPTFQTQAFYDL
                     AAKYGAAIVYAVDDEAPEWPRIDEPTADFTYARLMSSREDEPTGMTSDELDGVVVQTR
                     NWAKRGDVFAYFIAGAKVRNPAAAMALIEKLKG"
     gene            2086..2385
                     /locus_tag="PLH31_02135"
     CDS             2086..2385
                     /locus_tag="PLH31_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88077.1"
                     /translation="MRRVLALLLVSLALASPASADQKPAPKAAPPPVVVAAPQTALQP
                     LIPLASGRSGGDTGQCRAACARSYYFCMAGGDDTCPGNWAQCNARCTSTYKRLGS"
     gene            complement(2394..3062)
                     /gene="yihA"
                     /locus_tag="PLH31_02140"
     CDS             complement(2394..3062)
                     /gene="yihA"
                     /locus_tag="PLH31_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQR88078.1"
                     /translation="MTEPNFAPPAGPVFTEEEIEAARVLFAQPVAFMMGAVNMDGLPH
                     PIAPEVCFAGRSNVGKSSLINGLVGQKYLARASNEPGRTREINFFLLAEKLRLVDLPG
                     YGFARASKTTVSKFQDLGRAYLRGRATLRRVYLLIDSRHGLKKVDQEALDALDTAAVS
                     YQIILTKADKIKPHEVEKVTAETQKAIAKRPAAFPRVLATSSEKGLGMPELRAEIARV
                     CLDE"
     gene            complement(3062..4915)
                     /gene="yidC"
                     /locus_tag="PLH31_02145"
     CDS             complement(3062..4915)
                     /gene="yidC"
                     /locus_tag="PLH31_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQR88079.1"
                     /translation="MQNDNKNTLMFIVSAFAILIGYQFFILGPQQKQAEAELRARKAA
                     EAKIVATQPGVVIGPDGRPAPLKLSRDAAKAQSPRLSVDTPALSGSISLRGARIDDLW
                     LKRYDQTTSKTSPPVELFRPEGAEHAWFADFGWAGANLPGLPDSRTVWTAAPGQVLRP
                     DSPVVMTHDNGQGLIFTRTISVDSDAMFTVADSVRNQGGQTLSLAPYASVQRQGIPDK
                     LGKNQIIHEGAIGVFGGADGKNHELKQSKYGKWKKDKPLETFTSKGGWTGVTDQYWLA
                     ALIPDQSQDVAAQYRVTQAGPVDIYDVNIVGPTKVLAPGQTLTQNTRLFAGAKTVPLL
                     RQYEFGGQPPAWWKFWDRRQAQVPRFDDAVDWGMFSFFTRPIFNILEMFYKLVGNFGV
                     AILLLTVALKLVLYPLADKSYESMAKMKKIAPEVEKLKAKLKDDPTKMQQEMMGLYAK
                     EKINPMMGCLPMLVQIPVFYSLYKVLTVTIEMRHAPFFGWIQDLSAPEPTSLFNLFGL
                     LPYDPGALPLIGAMIAHLGIWPLLYGFTMWLTTAMNPPAADPIQQKIFQWFPVIFTFT
                     LSGFAVGLVIYWCWSNVLTIFQQYIIMRRYKVENPIDQIIARFKGKSVTAT"
     gene            complement(4923..>5202)
                     /locus_tag="PLH31_02150"
     CDS             complement(4923..>5202)
                     /locus_tag="PLH31_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQR88080.1"
                     /translation="RAKRRLREAARLLLPLHGRPMYDYVFIARGGTRTREWGRLLDDV
                     KTALISLAAEHDRAEARARLAQSVDLPKDPSSDALPATPGAPDSSVSG"
BASE COUNT          904 a         1663 c         1739 g          896 t
ORIGIN      
        1 cgacgccatg gaggcctgta tcgaccgggg catgcgccag gacggcgtcc tgcccggcgg
       61 cctggacgtg aaacgccgcg cccgccagat ccaccagacc atccagggcc gcatggagcg
      121 ccagatcagc gaccccttgg cggccatgga cttcgtcaat ctgtgggcca tggcggtcaa
      181 tgaagagaac gccgccggcg gccgcgtggt caccgccccg accaatggcg cggcggggct
      241 ggttccggct gtgctgcggt tcttcgtgcg cttttataac ggcacgcccg agcagatccg
      301 cgtcttcctg ctgaccgccg ccgccatcgg cgcgctctac aagcgcaacg cctcgatcag
      361 cggcgccgag gtgggctgcc agggcgaggt gggcgtggcc tgctcgatgg ccgccgccgg
      421 cctgtgtgcg gccttggggg ggaccaacgc ccagatcgag aacgccgccg agatcggcat
      481 ggagcataac ctcggcctca cctgcgatcc catcggcggg ctggtgcaga tcccctgcat
      541 cgagcgcaac gccatgggcg ccatcaaggc catcgacgcc gcccgtctgg ccctgctggg
      601 cgacggccag cactcggtct ccctcgacaa ggtgatcgcc acgatgaagc gcaccggcga
      661 ggacatgaac gagatctaca aggaaacatc catgggcggg ctggcggtgg ggctgtcggt
      721 caaccgcgtg gagtgctgag gtgaagcggc gcggcgtctg aattgccgtc gtgctgctgg
      781 cgctggcggc ggtcccggta gcccgttgta cgcgagaata cttcactgtg gacgcctgct
      841 tggacagcgg cggggcctgg atcgagcaga tcgacaaatg ctcgcacgat caggccgagc
      901 tcgaccggta caagccgcgc tgaccctcag tcgcggagtt tatcctgggg gggcgtttgg
      961 ccggacccag ggaacaactc ccccgaaggg gagcatctta ctggttcggc ggttcttgat
     1021 ccttcccctc cggggcagac ggcccggagg gctgtagggg gctggccgca acggccacct
     1081 ccacaagagc tgccacctct tcaagaccgg ccatgacacg gttgaagttg acgtcttcgc
     1141 tctcagtcat cgtttttacc ccgatccatg atgaaacgct ctggtcccgc cttcgccttt
     1201 tccctcgacg ccccgtcaaa gctaacctaa cttcaaaaag aacaggaagg gaacagatat
     1261 gtccaacggc gtgatccgca ccggcatcgg cggctggacg tttgagccct ggcgcggcgt
     1321 gttctttccc gacacggtca agcagaagga cgagctgaaa tacgcctcca gccagctgac
     1381 cagcatcgag atcaacggca cctactattc caccttcaag cccaacagct ggatgaagtg
     1441 gcgcgacgag acgcccgatg atttcgtctt tgcggtcaag gccagccgct actgcaccaa
     1501 tcgcaaggtg ctgtcggaga acaacgactc gctggagaaa ttcctgaccc aggggctgga
     1561 agagctgggc gacaagctgg ggcccatcaa ctggcagttc atggccacca agaagttcga
     1621 tccggtcgac ttcgagggct ttctgaagct gctgcccaga gagatcggcg gccggccggc
     1681 gcgccatgcg ctggaggtgc gcagtcccac cttccagacc caggccttct acgatctggc
     1741 cgccaaatat ggcgcggcca tcgtctatgc cgtggatgac gaggcgcccg agtggccgcg
     1801 tatcgacgag ccgaccgccg acttcaccta tgcccgcctg atgtcaagcc gcgaggacga
     1861 gccgaccggc atgacctccg acgaactgga cggggtagtg gttcagacgc ggaactgggc
     1921 caagcgcggc gacgtgttcg cctatttcat cgccggggcg aaggtcagga acccggcggc
     1981 ggcgatggcg ctgatcgaga agctgaaagg ctgacccttt cccccttgcg ggagagggaa
     2041 acatggttag ccaaacctta accgcccccg cgctaggctc cgcccatgcg ccgcgtcctc
     2101 gccctgctgc ttgtgtctct cgccctggcc tcgccggcca gcgctgacca gaaacccgcg
     2161 cccaaggcgg ccccgccacc ggtcgtagtc gcggcgccgc agacggccct tcagccgctg
     2221 atcccgttgg cctctggccg ctcgggcggc gacaccggcc agtgccgggc cgcctgcgcg
     2281 cgatcctatt atttctgcat ggcaggcggc gacgatacct gccccggcaa ttgggcgcag
     2341 tgcaatgcac gctgcacctc gacctacaag cggctgggct cctaagcccg cgcctactcg
     2401 tccaggcaga cccgggcgat ctcggcccgc agttccggca tgcccaggcc cttttccgac
     2461 gaggtggcca gcacgcgcgg gaaggccgcg ggacgcttgg cgatggcctt ctgcgtctcg
     2521 gcggtgacct tctcgacctc gtgcggcttg atcttgtcgg ccttggtcag gatgatctgg
     2581 taggaaaccg ccgccgtatc cagggcgtcc agagcctcct ggtccacctt cttgaggcca
     2641 tggcgcgagt cgataagcag atagacgcgg cgcagggttg cccggccgcg cagataggcc
     2701 cggcccaggt cctggaactt gctgaccgtg gtcttggagg cgcgcgcgaa gccatagccc
     2761 ggcaggtcga ccaggcgcag cttctcggcc agcaggaaga aattgatctc ccgcgtgcgg
     2821 cccggctcgt tggaggcgcg ggccaggtac ttctgcccca ccagcccgtt gatcaggctg
     2881 gacttgccga cgttggagcg accggcaaag cacacttccg gcgcgatcgg atggggcagg
     2941 ccgtccatgt tgacggcccc catcatgaag gccaccggct gggcgaacag cacccgcgcc
     3001 gcctcgatct cttcctcggt gaagacggga ccggcaggcg gcgcgaagtt gggttcagtc
     3061 atcaggtcgc cgttacgctc ttgcccttga agcgggcgat gatctggtcg atcgggttct
     3121 cgaccttgta gcggcgcatg atgatgtact gctggaagat ggtcaggacg ttggaccagc
     3181 accagtagat caccaggccc acggcgaagc ccgacagggt gaaggtgaag atcaccggga
     3241 accactggaa gatcttctgc tggatcgggt cggcggccgg cgggttcatg gccgtggtca
     3301 gccacatggt gaagccgtac agcagcggcc agatgcccag gtgggcgatc atggcgccga
     3361 tcagcggcag cgcgcccgga tcatagggca gcaggccaaa caggttgaac aggctggtcg
     3421 gctcgggggc cgacaggtcc tggatccatc cgaagaacgg cgcatgccgc atttcgatgg
     3481 tgaccgtcag caccttgtac agcgagtaga acaccgggat ctgcaccagc atgggcagac
     3541 agcccatcat cggattgatc ttttccttgg catacaggcc catcatctcc tgctgcatct
     3601 tggtcggatc gtccttgagc ttggccttca gcttttcgac ctcgggggcg atcttcttca
     3661 tcttggccat cgactcgtag ctcttgtccg ccagcggata gaggacgagc ttgagggcca
     3721 cggtcagcag caggatggcg acgccgaagt tgccgaccag cttgtagaac atctccagga
     3781 tgttgaagat cgggcgggtg aagaagctga acatgcccca gtcgacggcg tcgtcgaagc
     3841 gcggcacctg ggcctggcgg cggtcccaga acttccacca ggcgggcggc tgaccgccga
     3901 actcgtactg gcgcagcaat ggcacggtct tggcgccggc aaacaggcgg gtgttctggg
     3961 tcagggtctg gccgggggcc agcaccttgg tcgggcccac gatattgacg tcatagatgt
     4021 cgaccgggcc agcctgggtg acgcgatact gggccgccac gtcctgcgac tggtcgggga
     4081 tcagggccgc cagccagtac tggtcggtga cgccggtcca gccgcccttc gaggtgaagg
     4141 tctccagcgg cttgtccttc ttccacttgc cgtacttgga ctgcttgagc tcgtggttct
     4201 tgccgtccgc gccgccgaac acgccgatgg cgccttcgtg gatgatctgg ttcttaccca
     4261 gcttgtcggg aatgccctgg cgctggaccg aggcgtaggg ggccagagac agggtctggc
     4321 cgccctggtt gcggacgctg tcggcgacgg tgaacatcgc atcgctgtcg accgagatgg
     4381 tgcgggtgaa gatcagaccc tggccgttgt catgggtcat gaccaccggc gagtcgggac
     4441 gcagcacctg gcccggcgcg gcggtccaga cggtgcggct gtcgggcagg cccggcaggt
     4501 tggcgccggc ccagccgaaa tcggcgaacc aggcgtgctc ggcgccctcg gggcggaaca
     4561 gctcgaccgg cggcgaggtc ttggaggtgg tctggtcgta gcgcttgagc cacagatcgt
     4621 cgatccgggc gccgcgcagc gagatcgaac ccgacagcgc cggcgtatcc accgacaggc
     4681 gcgggctctg ggccttggcc gcgtcgcgcg acagcttcag cggcgcggga cgaccatcgg
     4741 ggccgatcac cacgcccggc tgggtagcga cgatcttggc ctcggccgcc ttcctggcgc
     4801 gcagctcggc ctcggcctgc ttctgctgcg gacccaggat gaagaactgg tacccgatca
     4861 ggatcgcgaa cgcgctgacg atgaacatca acgtgttttt gttgtcgttc tgcatggatg
     4921 acttaaccgg aaacagagga atcgggcgcg cctggggtgg caggtaaagc gtctgaagat
     4981 gggtctttgg gaagatcgac ggattgagcc agcctggccc gagcctccgc gcgatcatgt
     5041 tcggccgcga ggcttatcag cgcggttttc acatcgtcaa gcaaacgccc ccattcgcgg
     5101 gtgcgggtgc cgccgcgcgc gatgaataca tagtcgtaca tgggacggcc atgaaggggt
     5161 agcaacagtc tcgcggcctc gcgcaggcgg cgcttggcgc gg
//
LOCUS       DAOMEO010000056         5159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2052_length_5159_cov_2.750392, whole genome shotgun sequence.
ACCESSION   DAOMEO010000056 DAOMEO010000000
VERSION     DAOMEO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_5159_cov_2.750392"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1802
                     /gene="nusA"
                     /locus_tag="PLH31_02155"
                     /pseudo
     CDS             121..1802
                     /gene="nusA"
                     /locus_tag="PLH31_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
     gene            1860..2169
                     /locus_tag="PLH31_02160"
                     /pseudo
     CDS             1860..2169
                     /locus_tag="PLH31_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515417.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2180..2427
                     /locus_tag="PLH31_02165"
     CDS             <2180..2427
                     /locus_tag="PLH31_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQR88081.1"
                     /translation="DGADDGRRKMLTAVYKAPNSPRILGMFTSDELGLALGGENVIHT
                     ALLAGRGTDRWTLDVERLSGFRPLLPPTWSASWREKP"
     gene            2625..>5159
                     /gene="infB"
                     /locus_tag="PLH31_02170"
     CDS             2625..>5159
                     /gene="infB"
                     /locus_tag="PLH31_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQR88082.1"
                     /translation="MSDENDNGRPGTPGGRAPITLKPRQGSVSAGVVKQSFSHGRTKT
                     VVVETKRTRPHAAPAGNLAAPSSAERRHTNEPRPSQSSGGGGSAGGLSQDEMRARQRA
                     IEAARENQDRQATQRAADEARARAAEAAARDAAAKAAAVAKAAAEPAPAPTPAPAPAP
                     VAAAPAAQAPVAPAPVAQAPAPVAPPAPVAPPVAAAPAAPRAEAPRPAAPSSQTRTYE
                     PSRERRDDRTSTTTYRPPAGERPPQGDRPQGDRPFNQRAPRQDGPYNQRAPRPDAAPR
                     GPRPEGAGGFRNDRPQGDRPQGDRPQGDRPQGDRPTQTVRYSALAPRPAPGARPGPGG
                     PQRGPRPGVPAAAPATPEVQRAMRSAPRPGGEVSRRPEDDDDRRKAAAPGKAVSRAKG
                     APVRREGRLTIQTVAGDGDSADRMRSLASVRRAREREKEKRRGGPAEVVKVSREVVIP
                     DVITVQELSNRMAVRGVEIIKFLMRQGVMLKINDVIDNDTAELVATEFGHTVRRVSEA
                     DVEEGFIGAEDIDDHLESRPPVVTVMGHVDHGKTSLLDALRKADVAGGEHGGITQHIG
                     AYQVRLENGEKVTFLDTPGHAAFSQMRARGANITDLVILVVAGDDGVMPQTIEAIKHA
                     RAAEVPIIVAVNKMDKPGSDPTRVVNELLQHEIVVESLGGETQIVEVSAKTGQGLDEL
                     IERILLQAEVLDLKANPDRTADGVVIEAKLDKGRGAVSTVLVNRGTLKRGDIVVAGSQ
                     FGRVRALLNERNEQLTEAGPATPVEILGLDGVPSPGDTFAVVENEARARELTEYRIRQ
                     KREKSMHPVGAGATSMADMMAKLQDKKFRELPLVIKADVQGSAEAII"
BASE COUNT          919 a         1767 c         1752 g          711 t
ORIGIN      
        1 cggcggagac gcagacgagg atgacgtcgc cgccgacggc ggagacgcag acgaagaaga
       61 tctcggcgac gacgccgaag acgaagattt tgacctgtcc gaaagtgaag aggactgacc
      121 atggccatcg gcatttccgc caaccggctc gagctgctgc agatcgctga cgcggtcgcg
      181 cgcgaaaaag gcatcgagaa ggaagtcgtc atcgaggcga tcgaggacgc cctgcagaag
      241 gcggctcgcg cccgttacgg cgccgagcac gacattcggg tgaagatcga tccccgcacc
      301 ggcgagacct cgcagaagcg gatcatcacc gtggtcggcg acgaccagga gatcgaaggc
      361 gagatcggcc aggtccagct gtcgattgcc aagcgcacct ggcgcgacgc cgaaatcggc
      421 aaggtctatg aagaagacct gccgccgttc gagatcggcc gcgtccagac ccagatggcc
      481 cgccaagtgg tcatgcataa ggtccgcgaa gccgagcgcg agcgccagta cgacgagtac
      541 aaggaccgcg ccggcgagat catcaacggc agcgtcaagc gcgtcgaata tggcaatgtc
      601 atcgtcgacc tgggccgtgg cgaaggcatc atgcgccgcg accagtcgat cccgcgcgag
      661 aacttcaacg tcggcgaccg catccgcgcc tatatctacg acgtccgtcg cgagaccaag
      721 cgcgcgccca cggcggcttc atggcaaagc tgttcgcgca ggaagtgccg gaagtctatg
      781 acggcgtcat cgagatccgc gccgtggccc gcgacccggg ttcgcgcgcc aagatggccg
      841 tgatctccaa tgacggctcg atcgaccccg tcggcgcctg cgttggcatg cgcggttcgc
      901 gcgtgcaggc ggtggtggcc gagctgcagg gcgaaaagat cgacatcatc cagtggtccg
      961 aggacgaggc gaccttcatc gtcaacgccc tggccccggc cgaggtctcc aaggtcgtga
     1021 tggacgagga agacgagcgc gtcgaagtcg tggtgcccga tgagcagctg tcgctggcca
     1081 tcggccgtcg cggccagaac gtgcgcctgg ccagccagct gaccggctgg cagatcgaca
     1141 tcatgacgga aagccaggag agcgagcgcc gtcagaagca gttcaccgag accacggccc
     1201 tgttccagga agccctggac gtcgacgagg tcatcgccca gctgctggtc accgaaggct
     1261 tcgctgcggt ggaagatgtg gcctatgtcg agccgcacga aatcgcggcc atcgaaggct
     1321 tcgacgacga gaccgccgac gagctgcaga cccgcgcccg cgaattcctc gacaaggaag
     1381 ccgccgctct cgacgccaag cgcgtcgagc tgggggtcga ggattccgtg ctcgagatcg
     1441 aaggcgtcac cctggccatg gctgtggccc tgggcgaagg cgatgtgaag accgtcgagg
     1501 atctggccgg tctggtgccc gacgacatgc gcggctggtt cgaaaccaag gacggcgccc
     1561 gcgttcgcga gccgggcatc ctggaaagct tcaacctgtc gcccgaagac gccgaagcgc
     1621 tgatcatgcg cgcccggatc gtcatgggct gggtcgaggc cccgccggag ccggaatacg
     1681 aagagcccgt ctatgacgag gccgaattcg agggcgaggc cccggaagct gagggcgagg
     1741 ctgaagtcga agtcgaagcc gaagaagagc ccgccgccgt cgaagagacg caggaaggct
     1801 aagccagatg tcccgacctt tcccgctcgc cggggaaggt cgggcctttc gcgagacgca
     1861 tgaccgagcc cgacgcgccc aagacccacg ctgaagcctc ccgcctgcgc aaggacgtcg
     1921 tcctgggcga ggcgacggac gaggcgcggc tgatccgctt tgtcgagggg ccgggcggaa
     1981 tcgtggttcc agacctggcg cgcaagctgc cgggccgcgg gatctgggtc ggcgccgacc
     2041 gccagtcggt gaccttggcc gccaagaagg gtctgttttc gcgctcggcc aagaccaagc
     2101 tgaccgctcc ggccgacctc gccgaccagg tcgaggccct gctggtcagg cgcgttctgg
     2161 acggccttgn nnnnnnnnnc cgacggcgcc gatgatggcc ggcgcaagat gctgaccgcc
     2221 gtctacaagg cccccaatag cccgcgaatt ctcgggatgt tcacatccga cgaattgggt
     2281 ttggccttgg gcggggagaa tgtgatacac acggcgcttc ttgccgggcg cggcactgat
     2341 cgctggactt tggacgtcga gcgtttatca ggctttcgcc cgctactccc cccgacctgg
     2401 tcggcgagtt ggcgcgagaa gccctagggc ctttcgattg aaccggcgtg gccggtctga
     2461 tcgcaaggct ctagaatctt tgagatggtg cgcccgcaag gtcgcgcccg tgagtgaagg
     2521 gtcgggaaac gcgctttctt cgtgaaagta cgtccgggcc cctttctcgc atggaacgaa
     2581 cggtttttgg ccaggctttg tctggcggaa gtacaagcga gcgcatgagc gacgagaacg
     2641 ataacggtcg gcccggcacc ccaggtgggc gcgcgcccat cacactgaag ccccgccagg
     2701 gctcggtgag cgccggcgtg gtgaagcaaa gcttcagcca cggccgcacc aagacggttg
     2761 tggtggaaac caagcggacc cgtccgcacg ccgcgccggc cggcaacctg gccgcgccgt
     2821 cctcggccga gcgccgtcac accaacgagc cgcgcccgtc ccagtcttcg ggtggcggcg
     2881 gttcggcagg gggcctctcg caggatgaga tgcgcgcccg ccagcgcgcc attgaagcgg
     2941 ctcgcgagaa ccaggaccgt caggcgacgc agcgcgccgc cgacgaggcc agggcccgcg
     3001 ccgccgaagc cgcggctcgc gacgctgccg ccaaggctgc ggccgtcgcc aaggccgccg
     3061 ccgaacctgc gcccgcgccg accccggcgc cggcgcccgc tccggtggct gctgctccgg
     3121 cggctcaggc ccccgttgct ccggccccgg ttgctcaggc tcctgcaccc gttgcgcccc
     3181 cggcgcctgt cgcgcctccg gtcgctgctg ctccagctgc gccgcgcgcc gaggcgccgc
     3241 gtccggcggc tccgtccagc cagacgcgca cctacgagcc cagccgcgaa cgtcgcgacg
     3301 accgcacgtc gaccaccacc tatcgtccgc ccgccggcga gcgcccgccc cagggtgatc
     3361 gtccccaggg cgaccggccg ttcaaccagc gcgctccgcg ccaggacggc ccctacaatc
     3421 aacgcgcgcc gcgtcccgac gctgcgccgc ggggtccgcg tccggaaggc gccggcggtt
     3481 tccgcaacga tcgtccgcaa ggggaccgtc cgcaaggcga ccggccccag ggtgatcgtc
     3541 cgcagggcga tcgtccgacc cagaccgtgc gctattcggc tctggccccg cgcccggcgc
     3601 ccggcgcccg tccggggcct ggtggtccgc agcgcggtcc gcgtcccggc gttccggccg
     3661 ccgctccggc gaccccggaa gtccagcgcg ccatgcgctc ggcgccgcgt cccggcggcg
     3721 aagtcagccg tcgtcccgag gatgacgatg atcgccgcaa ggccgccgcc cccggcaagg
     3781 ccgtgtcgcg cgccaagggc gctccggtgc gccgcgaagg ccgcctgacc atccagaccg
     3841 tggccggcga cggagattcc gccgaccgca tgcgctcgct ggcctcggtt cgccgggccc
     3901 gtgaacgtga aaaggaaaag cgccgcggcg gtcccgccga ggtcgtcaag gtcagccgcg
     3961 aagtggtcat ccccgacgtc atcaccgtgc aggagctgtc caaccggatg gccgtccgcg
     4021 gtgtcgagat catcaagttc ctgatgcgtc agggcgtgat gctgaagatc aacgacgtca
     4081 tcgacaatga caccgccgag ctggtggcga ccgaatttgg tcacaccgtg cgccgcgtgt
     4141 cggaagccga cgtcgaagaa ggcttcatcg gcgccgagga catcgacgat cacctggagt
     4201 cgcggccccc ggtggtcacg gtcatgggtc acgtcgacca cggcaagacc agcctgctgg
     4261 acgcgctgcg caaggccgat gtggccggcg gcgaacatgg cggcatcacc cagcacatcg
     4321 gcgcttacca ggttcgcctg gaaaacggcg agaaggtcac cttcctcgac accccgggcc
     4381 acgccgcctt ctcgcaaatg cgggctcgcg gcgccaacat caccgacctg gtgatcctgg
     4441 tggtggccgg cgacgacggc gtcatgccgc agaccatcga agcgatcaaa cacgcccgcg
     4501 ctgcggaagt gccgatcatc gtggccgtca acaagatgga caagccgggc tcggacccga
     4561 cccgcgtggt caacgagctg ctgcaacacg agatcgttgt cgaaagcctg ggcggcgaaa
     4621 cccagatcgt cgaggtctcg gccaagaccg gccagggcct ggacgagctg atcgagcgca
     4681 tcctgctgca ggccgaggtt ctcgacctga aggccaaccc cgaccgcacc gccgacggcg
     4741 tggtgatcga ggccaagctc gacaagggtc gcggcgcggt gtccaccgtg ctggtcaatc
     4801 gcggcacgct gaagcgcggc gacatcgtcg tggccggcag ccagtttggc cgggttcgcg
     4861 ccctgctcaa cgagcgtaac gagcaactga ccgaggctgg ccccgccacc ccggtcgaga
     4921 ttctcggcct ggacggcgtt ccctcgcctg gcgacacctt cgccgtcgtg gaaaacgaag
     4981 cccgcgctcg cgagctgacc gaataccgca tccgccagaa gcgcgagaaa tcgatgcatc
     5041 cggttggcgc gggcgccacc agcatggccg acatgatggc caagctgcag gacaagaagt
     5101 tccgcgaact gccgctggtc atcaaggccg acgtgcaggg ctcggccgag gcgatcatc
//
LOCUS       DAOMEO010000057         5118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2063_length_5118_cov_2.574166, whole genome shotgun sequence.
ACCESSION   DAOMEO010000057 DAOMEO010000000
VERSION     DAOMEO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_5118_cov_2.574166"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLH31_02175"
     CDS             complement(<1..444)
                     /locus_tag="PLH31_02175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR88083.1"
                     /translation="MTPLQPLYATLFALALAVAPCQAASPVSPPGTPAEAKDIDVFVR
                     KALAQAQVVPGLSVTVVQADKVVMTEGYGVADLRTGRTVDADTGFYIASATKAFTALA
                     IAAMAQRGELALDAPLSGWMGKSSLPADLASSTSLTDLLSHRSGLE"
     gene            complement(523..1242)
                     /locus_tag="PLH31_02180"
     CDS             complement(523..1242)
                     /locus_tag="PLH31_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR88084.1"
                     /translation="MSVSAILSVSSDHRWVLTHYEPGESSRAHRHDTAQTSLLLAGGY
                     IEESPEGILDVEGLHLSRKESGFEHQNQFSDAGALILSVQGPDSAAGAAGYHVATCGS
                     REAGHAILTRTAAGAALPGAVGGRLGEDAGRRRPAWISQARERLIAEPHAPIATLART
                     IGRHPVAFARAFRGVYGRGPAQYRHDWRVANALHQIVRSQAPLAEVAIVEGFSDQAHM
                     TRAIRQACGWSPGALRRLFAA"
     gene            complement(1533..2711)
                     /locus_tag="PLH31_02185"
     CDS             complement(1533..2711)
                     /locus_tag="PLH31_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HQR88085.1"
                     /translation="METKAAGPGAFARFDLTRVPSPCFVIDEVAVRRNLAMLKAVGDG
                     SGARVLLALKAFSMWSLAGVVAEYLDGVCASGLWEARLAREFYHGHLTTYSPAYKRED
                     LPAILRISDSVIFNTPDQIARFADLIAAAREQGEVFDIGLRLNPEHSEAEVAKYDPCQ
                     RGSRLGFPVSQLRPEHLEGVDGLHIHALCEQDFAPLQRIWQSLEPRLAGMLGQLKWLN
                     LGGGHHVTRADYQTADLIAFLAGIRQRYGLEVYIEPGEAIALDAGILVGEILDTFDNS
                     VPVAITDISATCHMPDVIEAPYRPAMLGEVEDGAGVTVRIGGPSCLAGDIIGDYGFAT
                     RPVPGQRIAFLDQAHYSMVKTNTFNGVPLPAIALWNSDTDALELVRQFDYAEFRDRLS
                     "
     gene            complement(2714..3919)
                     /locus_tag="PLH31_02190"
     CDS             complement(2714..3919)
                     /locus_tag="PLH31_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HQR88086.1"
                     /translation="MSRVLVIGAGGVGSVAVHKMAMNADVFSHITLASRTKSKCDAIA
                     ASVKQRTGVTVDTAAIDADDVAATTALIQAVKPALVVNLALPYQDLNIMDACLAAGVN
                     YLDTANYEPRDEAKFEYKWQWAYQDRFKDAGLMALLGSGFDPGVTSVFTTYTKKHLLD
                     RIDTLDILDCNGGDTGLPFATNFNPEINLREVTAPSRHWENGQWIEGPALSHKQVFDF
                     EQVGEKNMYLMYHEELESLVKFYPEIKRIRFWMTFGDSYLKHLELLGNIGMTSIEPMM
                     FQGREIIPIEFLKALLPEPSSLGPITKGKTNIGTIATGMKDGKARTVYVNNICDHEEA
                     YAETNNQAVSYTTGVPAMIGAALMLTGQWKGAGVFNMEQLDPDPFMDMLNKHGLPWKV
                     QDLAAPLAF"
     gene            complement(4040..>4565)
                     /locus_tag="PLH31_02195"
     CDS             complement(4040..>4565)
                     /locus_tag="PLH31_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HQR88087.1"
                     /translation="WPLSFDYAEEIYQYSGFDPARVRVLQTLDFAGTPLKRPYAVPVA
                     WARQIGKGRLFFTNLGHTPSTWDDPRFRRQVAEAIRWAGKRTRGAAKPDVARQATWQF
                     RALLAYQPVAGRDDTAILERLEKADPAWREATASRIAALQPAYPAKPESDRVPFETAY
                     RAILAEVLMRAQSR"
     assembly_gap    4566..4575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4576..4945)
                     /locus_tag="PLH31_02200"
     CDS             complement(<4576..4945)
                     /locus_tag="PLH31_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQR88088.1"
                     /translation="MRRLIAFLVALTTLAMASTAAAAEKPLRVLYLDQSVGWKHAPVV
                     RPEHGGLALSETAMIAIGQESGTFVAEVTQDAREITPQRLESVDVLVFYTTGALPISA
                     EAWSAVQARLAAGKLGFVGLH"
BASE COUNT          866 a         1665 c         1697 g          880 t
ORIGIN      
        1 ttccagaccc gacctgtgac tgagcagatc cgtcaggctg gtcgagctgg cgaggtcggc
       61 ggggagcgag ctcttgccca tccagccgct gaggggcgcg tcgagggcca gctccccccg
      121 ttgggccatg gcggcaatcg ccagagccgt gaaggccttg gtcgccgagg cgatatagaa
      181 gcccgtgtcg gcgtcgacag tgcggccggt ccgcagatcc gccaccccgt agccttcggt
      241 catgaccacc ttgtctgcct ggacgaccgt cacggaaagg ccgggcacga cctgggcctg
      301 ggccagcgcc ttgcgtacga agacatcgat gtccttggct tcagcgggcg tccctggcgg
      361 ggatacgggt gaggccgcct ggcagggcgc aacggccagg gcgagcgcga aaagcgtggc
      421 gtacagtggt tgaagcgggg tcatgtgcga ggtctcgtgg gcgaaagcga cagctacacc
      481 atggtctcgc gccagggtct tgaacggacg aaaccgtcgg acctaggccg caaacagccg
      541 acgcagcgcg cctgggctcc atccgcaagc ctggcggatg gcccgggtca tgtgggcctg
      601 atccgaaaac ccttcaacaa tcgccacctc cgccagcggc gcctgtgaac ggacgatctg
      661 atggagcgca ttggctacgc gccagtcgtg ccggtactgc gccggaccgc gcccgtacac
      721 gcctcggaac gcccgggcga aggcgaccgg gtggcggccg atcgtgcgcg ccagggtggc
      781 tatgggcgcg tgcggttcgg cgatcagccg ctctcgcgcc tgggaaatcc aggctggacg
      841 tctgcggccg gcgtcctcgc ccaggcgccc gcccaccgcg ccgggcaagg ccgcgccggc
      901 cgccgttcgg gtcaggatcg catggcccgc ctcccgcgat ccgcacgtcg cgacatgata
      961 gcccgccgcg cccgccgcag agtccgggcc ctgcaccgac aggatcagcg cccccgcgtc
     1021 actgaactgg ttctgatgct cgaagccgct ttccttgcgg ctcaggtgaa ggccttcgac
     1081 gtcgaggatc ccttcgggac tttcctcgat atatccgccc gccagcagca gggaggtttg
     1141 cgccgtatcg tgacggtgcg cccgtgagga ctcccccggc tcgtaatgcg tcagcaccca
     1201 gcgatggtcg gacgatacag acaagatagc gctgacggac atggtgagag cctcccattg
     1261 gttagaggct ccgccgacga catttggagg cgctctctgg aaattttttt ccagtcgggg
     1321 ttctggagcc aagggacagc gggcctcgac ttcggcggga cgatgccggg ctcggtcgtg
     1381 cgtaagtatt accggaggtc ccgcatcccg accttcagat cgccaccgtc agccattgtc
     1441 gggcctagcg aacacacgcc cttgtggcgg gtatgacgga gtttaagaga tcggccgtca
     1501 tcgcgatcct gaatgtcgat ccgctcaaga cttcaagaca accggtcgcg gaactcggca
     1561 tagtcgaact ggcgcaccag ttcgagggcg tcggtgtcgc tgttccacag ggcgatggcc
     1621 ggcaggggca cgccgttgaa ggtgttggtc ttgaccatcg aatagtgcgc ctggtccagg
     1681 aaggcgatgc gctgccccgg aaccggccgc gtggcgaagc catagtcgcc gatgatgtcg
     1741 ccggccaggc acgacggccc gccgatcctc accgtcacgc cggccccgtc ctcgacctcg
     1801 cccagcatcg cgggccggta cggcgcctcg atgacgtcgg gcatgtggca ggtggccgag
     1861 atgtcggtga tggcgaccgg tacgctgttg tcgaaggtgt cgaggatctc gccgaccaga
     1921 atgccggcgt cgagcgcgat ggcctcaccc ggttcgatat agacctccag cccgtagcgc
     1981 tggcggatcc cggccaggaa ggcgatcagg tcagcggtct ggtagtcggc gcgggtgacg
     2041 tggtgaccac cccccaggtt cagccacttc agctggccca gcatgcccgc gagccggggc
     2101 tccagagact gccagatccg ctgcagcggc gcgaaatcct gctcgcacag ggcatggatg
     2161 tgcagaccat cgacgccctc cagatgctcg ggacgaagtt gcgacaccgg aaagcccagc
     2221 cgcgaaccgc gctggcaggg atcgtacttg gcgacctcgg cctcgctgtg ctcggggttc
     2281 aggcgcaggc cgatatcgaa cacctcgccc tgctcccgcg ccgccgcgat cagatcggca
     2341 aagcgcgcga tctggtcggg cgtgttgaag atcaccgagt cggagatccg caggatcgcg
     2401 ggcaggtcct cgcgcttgta ggccggcgag taggtggtca ggtggccgtg gtagaactcg
     2461 cgggccagcc gcgcctccca caggcccgag gcgcagacgc cgtccagata ttcggcgacg
     2521 acgccggcca gcgaccacat cgaaaacgcc ttcagcgcca gcagcaccct ggcgccagac
     2581 ccgtcgccca cggccttgag catggccagg ttgcggcgca ccgcgacctc gtcgatcacg
     2641 aaacagggcg acgggacgcg ggtcaggtca aagcgggcga atgcgccggg gccggcggcc
     2701 ttggtttcca tgactagaag gccaacgggg ccgccaggtc ctgcaccttc cagggcaggc
     2761 cgtgcttgtt cagcatgtcc atgaacggat cgggatccag ctgctccatg ttgaacacgc
     2821 ccgcgccctt ccactgaccg gtcagcatca gggccgcccc gatcatggcc ggaacgcccg
     2881 tggtgtagct gaccgcctga ttgttggtct cggcataggc ttcttcgtgg tcgcagatgt
     2941 tgttgacgta gaccgtgcgg gccttgccgt ccttcatccc cgtggcgatc gtgccgatat
     3001 tggtcttgcc cttggtgatg ggtcccagcg acgacggctc gggcagcagg gccttcagga
     3061 actcgatcgg aatgatctcg cggccctgga acatcatcgg ctcgatgctg gtcatgccga
     3121 tattgcccag caactccagg tgcttgagat agctgtcgcc gaaggtcatc cagaagcgga
     3181 tgcgcttgat ctcgggatag aacttcacga gggactcaag ttcctcgtgg tacatcaggt
     3241 acatgttctt ctcgccgacc tgctcgaagt cgaacacctg cttgtggctc agcgccggtc
     3301 cttcgatcca ctggccattt tcccagtgcc gcgacggggc ggtcacttcg cgcagattga
     3361 tctcgggatt gaagttggtg gcaaacggca ggccggtgtc gccgccgttg cagtccagga
     3421 tgtcgagcgt gtcgatccgg tccagaaggt gcttcttggt gtaggtggtg aacaccgagg
     3481 tcacgccagg atcgaagccg ctgcccagca gcgccatcag accggcgtcc ttgaagcggt
     3541 cctgataggc ccactgccac ttgtattcga acttggcctc gtcgcgcggc tcgtaattgg
     3601 ccgtgtcgag atagttcaca ccggccgcca gacaggcgtc catgatgttc aggtcctgat
     3661 agggcagggc cagattgacc accagggcgg gcttgaccgc ctggatcaat gcggtcgtgg
     3721 cggcgacatc gtcagcgtcg atcgcggcag tgtcgactgt cacgccggtg cgctgcttca
     3781 ccgaggcggc gatagcgtcg catttcgact tcgtgcgact cgccagcgtg atgtgcgaga
     3841 acacgtcggc attcatcgcc atcttgtgga cggcgaccga accaacgcca ccagcaccga
     3901 taaccagcac cctgctcatt tgggtttctc ctaacgccta tagcctccag cagcctgact
     3961 agcgcgggag gggggcgggg gtcttatcac gaggacagcg agtccgctaa ccaccgtagg
     4021 cagaagctgc gacggttctt tatctgctct gtgcccgcat caaaacttcc gcgaggatcg
     4081 cccgataggc cgtttcgaag ggaacacgat cgctttcggg cttggccgga taagcgggct
     4141 gaagcgcagc gatgcggctg gcggtggcct cgcgccaggc tggatcagcc ttttccaggc
     4201 gctccaggat cgccgtatcg tcgcggccgg cgaccggttg ataggccagc agcgccctga
     4261 actgccatgt cgcctgacgc gcgacgtccg gcttggccgc gccccgcgtg cgcttgcccg
     4321 cccaccggat cgcctcggcc acctggcggc ggaaacgggg atcgtcccag gtcgagggcg
     4381 tgtggcccag attggtgaag aacagtcgcc ccttgccgat ctggcgggcc caggccaccg
     4441 gcacggcata gggccgcttt agcggcgttc cggcgaaatc cagggtttgc aacacccgca
     4501 cccgggccgg gtcaaagccc gaatactgat agatctcctc ggcatagtcg aagctgagcg
     4561 gccacnnnnn nnnnntgtgc agacccacaa agcccagctt gcccgccgcc agccgcgcct
     4621 gcaccgcgct ccacgcctcc gccgagatcg gcagggcgcc ggtggtgtag aaaactagca
     4681 catccacgct ctccagccgc tgcggggtga tctcgcgggc gtcctgggtg acctcggcga
     4741 cgaaggtccc gctttcctgg ccgatggcga tcatcgcggt ttcggacagg gccaggccgc
     4801 cgtgctcggg cctgaccacc ggggcgtgct tccagcccac ggactgatcc agatagagca
     4861 cgcgcagcgg cttttcggcg gcggcggccg ttgaggccat ggccagggtc gtcagggcga
     4921 ccagaaaggc gatgagccga cgcatctgct gcccctttgt cttcttcacc agcctctcga
     4981 cagccgcctg cagcgacggg tcctggcgac caagccgggc catttgaagc acggtcgcgg
     5041 caggggcgta aggggcgtcc ggtttggggg tcagaacttc ccgctctgga agtcctgaaa
     5101 cgcctgaacg atctcgtc
//
LOCUS       DAOMEO010000058         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2073_length_5099_cov_2.799960, whole genome shotgun sequence.
ACCESSION   DAOMEO010000058 DAOMEO010000000
VERSION     DAOMEO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2073_length_5099_cov_2.799960"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLH31_02205"
     CDS             complement(<1..190)
                     /locus_tag="PLH31_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQR88089.1"
                     /translation="MSLSVSARAVHHPLKAPFRISRGVKTAAEVVVVEVRSGDHIGRG
                     ESVPYARYGETPEQVVALL"
     gene            complement(234..455)
                     /gene="didA"
                     /locus_tag="PLH31_02210"
     CDS             complement(234..455)
                     /gene="didA"
                     /locus_tag="PLH31_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-induced SOS-independent cell division
                     inhibitor A"
                     /protein_id="HQR88090.1"
                     /translation="MTDYAMPQYHNAPRPSVSRRQGRPGRPTGWLGAINYGGVLLVWA
                     GLLLINPPLALKVFRERRADSPIRHDWRW"
     gene            579..893
                     /locus_tag="PLH31_02215"
     CDS             579..893
                     /locus_tag="PLH31_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HQR88091.1"
                     /translation="MSKPITITIPHQLGAVEARRRIENGFAQMAGQIPGGGADKVHQS
                     WNGDTMTFSAVAMGQTISGTMDVLGDMVKLDIVLPGMLGMIAGKIRDQLQKRGQLLLE
                     KK"
     gene            complement(906..1313)
                     /locus_tag="PLH31_02220"
     CDS             complement(906..1313)
                     /locus_tag="PLH31_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQR88092.1"
                     /translation="MADPLYNSPSPIVSGLRCRCPRCGEGKLFRGFLKLAPGCDRCGL
                     SYAFADPADGPAFFVMSGVGIVVIAVFAWVEVAYHPPIWVHFATVFPALLAGCLGTLR
                     PVKAWLVASQFIHKAEEGRFESVGRHDRDPRRL"
     gene            1396..2754
                     /locus_tag="PLH31_02225"
     CDS             1396..2754
                     /locus_tag="PLH31_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQR88093.1"
                     /translation="MPSNPSTAWLKRAEGLIGPAYKRLAQALEAAIAQGELQAGDQIP
                     AQREVARLVGVDFTTVTRAYALARERGLIEGTTGRGTFVRSQTGEDEAGLVDLSMNLP
                     PQPRSLNLSALLRETTGAVLARTDPAVLLAYHPGAGSLAQRAAAASWLAPTLGEIAPE
                     RVLIGSGAQTALSALLELLAAPGDAIIVEALTYPGLLTAAARRGLHVIPCPMDAEGLE
                     PGALARLCAAHAPRAIYCNPTFQNPTTATMSQARREAIAAVAKAAAVPILEDDAYGLL
                     PAAPLPALASLWPEGVFHIATTAKTLSPGLRIAFVVAPEDRVAETTRALHAIAQMPAP
                     LMAAVVTAWIRDGDALRVLDGVRREAVERRRLAAELLPAAVGGPESLHIWLDLPSGWS
                     RGRLPEAARARGLSLVDAESFRAPGVTREGVRLSLGAPGKAATLARALRELAGLLTAG
                     PA"
     gene            complement(2751..2957)
                     /locus_tag="PLH31_02230"
     CDS             complement(2751..2957)
                     /locus_tag="PLH31_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88094.1"
                     /translation="MAKLQPLQSFSLQETEDGYRLLVSAIGGDTLYVSITAEQMDDII
                     DSLELATGGEDDVAGEGDEDEGEF"
     gene            complement(3019..3651)
                     /locus_tag="PLH31_02235"
     CDS             complement(3019..3651)
                     /locus_tag="PLH31_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR88095.1"
                     /translation="MKTPTTRSPRKAKGQGSERREEILDAALALFGEKGVHAVSTRQI
                     AEQAGISQPALYAYFATKDDMAAELCVRAFAILVERMCAARTNHVPTPANFERAMRVY
                     IDFGMEHPNAYRVAFMIEKGPNGEFLDKTNSRPMIAGKEAFAMFCEIVGEYHVAGLTA
                     LDDVVVTAQALWAGLHGLVSLLIARPEFPWVERETLIAAHIAMLRRGALK"
     gene            3742..4833
                     /locus_tag="PLH31_02240"
     CDS             3742..4833
                     /locus_tag="PLH31_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR88096.1"
                     /translation="MRRSPQPLLQRAWRPLVFAAALPLLASCGGKAKEAEAPALPTPV
                     EAARVAAPNAAGTVSGTGTLERRREMALSFRIAGVLTRMTVEAGDSVRAGQVIATIDP
                     ANLDARQQQTSADLERARRDVERDKALFEKGYVSRQRLDDRQSALKSAQAAYSSASFD
                     RRWAQLVSPASGVVLARAVQTGEVVQPGQVVARVADLNSPLVLRLPLAARDAARVRVG
                     DTAQVKVEDLGGQSLDGTVTRVGEAADTRTGAVTVEIELAGRPDLRSGQIASAQLSVR
                     AAASAGNGFARIPAEAVLEAAGARAFVLRLDGGVARRTAVAFGGFDGDDALVSGLADG
                     AQVITAGAGFVSDGEKVRVVDPAKLAGGR"
     gene            4836..>5099
                     /locus_tag="PLH31_02245"
     CDS             4836..>5099
                     /locus_tag="PLH31_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88097.1"
                     /translation="MKFDLAGIAVHRWQFTLVAFGLLVMLGVNAFTSIPRSEDPHFPI
                     PIVVIRAVLPGAEPSEMEQLVVDPIEDAVDGLDNIDKVESTSLD"
BASE COUNT          792 a         1758 c         1772 g          777 t
ORIGIN      
        1 gcaggagggc gaccacctgc tccggcgttt ccccatagcg ggcatagggc acgctctcgc
       61 cgcgtccgat gtggtcgccg gagcggacct cgaccaccac gacctcggcg gcggtcttga
      121 ccccgcgcga gatgcggaac ggggccttca gcgggtgatg gacagcgcgg gcggagacgg
      181 acagcgacat gggccaaggc ttgtcactgt tcgccgaggt tggcgagacc ctcctaccag
      241 cgccagtcgt gacggatcgg cgagtcggcg cggcgctcgc gaaacacctt cagggccagg
      301 ggcggattga tcagcaggag gcccgcccag accagcagca cgccgccgta gttgatggcg
      361 cccagccagc ccgtgggacg acccggccgc ccctggcgac gcgacaccga cgggcgcggc
      421 gcattgtgat actggggcat ggcgtagtcg gtcatggcat cctccttgga tgccccgacc
      481 atcgccccta tctacgtcgg aaacggacgc atgcatcgcg accgaaccgt cagccgcttc
      541 ggggcgttga tcccgaaccc tcagccagag atccaagcat gtccaagccg atcacgatca
      601 ccatccccca ccagcttggc gcggtcgagg cgcggcgacg gatcgagaac ggcttcgctc
      661 aaatggccgg ccagattcca ggcggcggcg ccgacaaggt tcaccagtcg tggaacggcg
      721 acaccatgac cttctcggcc gtggccatgg gtcagacgat cagcggcacc atggatgtgc
      781 tgggcgacat ggtgaagctg gatatcgtcc tgccggggat gctgggcatg atcgcgggca
      841 agatcagaga ccagttgcag aagcgtggtc agctgctgct cgagaagaag taggctgacc
      901 ggggcctaga ggcggcgcgg atcgcgatcg tgccggccca cgctctcgaa ccggccttcc
      961 tcggccttgt ggatgaactg cgacgccacc agccaggcct tgacggggcg aagcgtgccc
     1021 agacagcccg ccagcaaggc cgggaacacg gtggcgaaat gcacccagat cggcgggtgg
     1081 taggcgacct ccacccaggc gaacacggcg atgaccacga tgccgacgcc gctcatcacg
     1141 aagaaggccg gaccatcggc cgggtcggcg aaggcatagc tcaggccgca gcgatcgcag
     1201 cccggggcca gcttcagaaa gcccctgaac agcttgccct cgccgcaacg cggacagcgg
     1261 cagcgcaggc ctgagacgat cggcgacggg gagttgtaga gcggatcggc cattgaaagc
     1321 ccttcctaca atcgtgcgac tgatacatac attacgggca caatgtatgc attcaggggt
     1381 cgcgagacaa ccgtcatgcc atcgaacccc tctaccgctt ggctgaaacg cgccgaaggg
     1441 ctgatcggcc cggcctacaa gcgtttggcg caggcgcttg aggcggcgat cgcgcagggc
     1501 gagctccagg ccggcgacca gatccccgcc cagcgcgagg tcgcgcggct ggttggcgtc
     1561 gatttcacca ccgtcacccg cgcctatgcc ctggcgcgcg agcgtgggct gatcgagggg
     1621 acgacggggc gcgggacctt cgtcaggagc cagaccgggg aggacgaggc gggtctggtc
     1681 gacctgtcga tgaacctgcc gccccagccc cgcagcctga acctcagcgc cctgctgcgg
     1741 gaaacgaccg gggcggtgct ggcccgcacc gatccggcgg tgttgcttgc ctatcacccc
     1801 ggcgccgggt cgctggccca gcgcgcggcg gcggccagct ggctggcgcc gactttgggc
     1861 gaaatcgcgc ccgagcgggt gctgatcggc agcggcgccc agaccgcgct cagcgccctg
     1921 cttgagctgc tggcggcgcc gggcgatgcg atcatcgtcg aggccctgac ctatcctggc
     1981 cttctgaccg ctgcggcccg acggggtttg cacgtcatcc cctgccccat ggacgccgaa
     2041 gggctggagc cgggcgccct ggcgcggctc tgcgctgcac atgcccctcg cgcgatctac
     2101 tgcaacccca ccttccagaa cccgaccacc gcgaccatga gccaggcgcg gcgcgaggcc
     2161 atcgcggccg tggcgaaggc ggcggcggtt ccgatcctcg aggacgacgc ctatggcctg
     2221 ctgccggcgg cgcccctgcc cgccctggcc agcctgtggc cggaaggggt gtttcacatc
     2281 gccactaccg ccaagaccct gtcgccgggc ctgcggatcg ccttcgtggt cgcgccggag
     2341 gatcgagtcg ccgaaaccac ccgggcgctg cacgccatag cgcagatgcc ggcgcctttg
     2401 atggccgccg tggtcacggc ctggattcgg gacggcgacg ccctgcgcgt gttggacggt
     2461 gttcggcgcg aggcggtcga acgacggcgg ctggccgccg aactcttgcc ggcggcggtg
     2521 ggcgggcccg aaagcctgca catctggctg gacctgccga gcggctggag tcggggccgg
     2581 ctgccggagg cagcgcgcgc gcgcgggctg tcgctggtcg acgctgaaag cttccgggcg
     2641 ccgggcgtca cgcgggaggg tgttcgcctg tcgctgggcg cgccgggcaa ggcggcgacc
     2701 ctggcgcggg ccttgcggga gctggcgggt ttgctgacgg cgggaccggc ctagaactcg
     2761 ccctcgtcct cgtcgccctc gccggccacg tcatcctcgc cgccggtcgc cagctccagc
     2821 gagtcgatga tgtcatccat ctgctcggcc gtgatcgaga catacagggt gtcgccaccg
     2881 atggccgaga ccagcaggcg atagccgtct tcggtctctt gcaggctgaa cgactggagg
     2941 ggctgcagct tggccatgga aggctccggt aaaatcaagg cgcggatata ggctcacgcg
     3001 cccggttttg ccacgcccct acttcagcgc cccacgtcgc agcatggcga tgtgggcggc
     3061 gatcagggtt tcgcgctcca cccagggaaa ctcgggtcgg gcgatcagca gcgagaccag
     3121 gccgtgcagt cccgcccaca gggcctgggc ggtcacgacg acgtcgtcca gcgccgtcag
     3181 gcccgccacg tgatattctc cgacgatctc gcagaacatc gcgaacgcct ccttgccggc
     3241 gatcatcggg cggctgttgg tcttgtccag gaactcaccg ttcgggccct tctcgatcat
     3301 gaaggccacg cggtaggcgt tggggtgctc catgccgaaa tcgatataga cccgcatggc
     3361 gcgctcgaaa ttggccgggg tcgggacatg gttggtgcgc gcggcgcaca ttcgctcgac
     3421 caggatggcg aaggcccgca cgcacagctc ggcggccata tcgtccttgg tggcgaaata
     3481 ggcatagagc gccggctgcg agatgccggc ctgctcggcg atctggcgcg tggacacggc
     3541 gtgaacgccc ttctcgccaa acagcgccag ggctgcgtcg aggatttctt cccgccgctc
     3601 gctgccttgc cccttggcct tgcgcggcga tcttgtggtt ggcgtcttca ttttccgacc
     3661 ttcatcatcc cgcgtcgcgc ttgacaagcc accacaatct atcactgata acttatcacc
     3721 gattgatttg gaactcggac gatgcggcgc tctccccagc cccttctcca acgcgcctgg
     3781 cggccgctgg tcttcgcggc cgccctgcct ttgcttgcct cgtgcggcgg caaggccaag
     3841 gaagccgaag cgccagcctt gccgacgccc gtcgaggccg cccgggtcgc cgcgcccaac
     3901 gccgctggaa cggtgtcggg gaccggcacg ctggagcgcc gccgcgagat ggcgctgtcg
     3961 ttccgcatcg ccggcgtgct gacccggatg accgtcgagg ccggcgacag cgtgcgggcc
     4021 ggccaggtga tcgccaccat cgatccggcc aatctggacg cccgccagca gcagacctcg
     4081 gccgacctgg agcgcgcccg ccgagacgtc gagcgcgaca aggccctgtt cgagaagggc
     4141 tatgtcagcc gccagcgact ggatgaccgc cagagcgcgc tgaaatccgc ccaggcggct
     4201 tacagttccg cgtcgttcga ccgccgctgg gcccagctgg tctcgccggc ctcgggcgtc
     4261 gtgctggccc gcgcggtcca gaccggcgag gtggtccagc ccggccaggt ggtggcgcgc
     4321 gtcgccgacc tgaacagccc gctggtcctg cgcctgccgc tggccgctcg cgacgccgcc
     4381 cgcgtgcggg tcggagacac cgcgcaggtc aaggtcgagg atctgggcgg gcagtcgctc
     4441 gacggaaccg tcacccgcgt cggcgaggcg gccgacaccc gcaccggagc cgtcacggtc
     4501 gagatcgaac tggcgggccg ccccgatctg cgcagcggcc agatcgctag cgcccagcta
     4561 tcggtccgcg ccgccgccag cgccggcaat ggctttgccc gcatccccgc cgaggccgtg
     4621 ctggaggccg ccggcgcccg cgccttcgtg ctgcggttgg acggcggcgt cgctcgccgg
     4681 acggcggtgg cctttggcgg ctttgacggc gacgacgccc tggtctcggg cctggctgac
     4741 ggcgcccagg tgatcacggc cggcgccggc tttgtcagcg atggcgagaa agtgcgcgtc
     4801 gtcgatccgg ccaagctggc cgggggccga tagtcgtgaa gttcgatctg gccggaatag
     4861 cggttcaccg ctggcagttc accctggtgg cgttcggcct gctggtgatg ctgggcgtca
     4921 acgccttcac cagcattccg cggtcggagg atccgcactt cccgatcccg atcgtcgtca
     4981 tccgcgccgt gctgccgggg gccgaaccct cggagatgga gcaactggtc gtcgatccta
     5041 tcgaggacgc ggtcgatggt ctggacaata tcgacaaggt ggaatcgacc agcctggac
//
LOCUS       DAOMEO010000059         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2074_length_5099_cov_2.409001, whole genome shotgun sequence.
ACCESSION   DAOMEO010000059 DAOMEO010000000
VERSION     DAOMEO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_5099_cov_2.409001"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1413)
                     /locus_tag="PLH31_02250"
     CDS             complement(430..1413)
                     /locus_tag="PLH31_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQR88098.1"
                     /translation="MAPVTETPTVERGAPATERLSTRLTGLARVTSGDVKEKTLKLVD
                     PARCRMWSRHNRRYDLLNEASCADLLESLRAQGEQEFPAIVRRVEDDPAFDYEVISGA
                     RRHWSVSYLRNVEHREIRYLIEERELTDEQAFRLADVENRARQDISDYERALDYRHAV
                     ETFYGGVAQRMAERLEVPKAWLSRFLDLGKLPTDVVEAFGDPRQLKERHARTLKPLLA
                     DDASRPKVMAEAKRLAGVQTAAVASGKGLLDPAKVVTLLVKATAAPASAKKPAAKGVV
                     SVTNDAGATLFTLARKGAKRVVLELSLDAEASNADFQAAFARELDRVRPKA"
     gene            complement(1471..2817)
                     /locus_tag="PLH31_02255"
     CDS             complement(1471..2817)
                     /locus_tag="PLH31_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR88099.1"
                     /translation="MADRLACRRLEPRGFRLGALLTLSSLYPTENLANSRHDVGYLQR
                     RRTMAKQALAESTASGPVLLAAMSALSDRAHDVITRLRATVFAPGEQKIVDLRFTVTK
                     AAEMVGRTSEAIRQAEADGRLPAPRLSATGRREGYSLSEVNHMRDVFGTRPRRGPDDP
                     PIVLAVQNFKGGVGKSTLTCHVAQFLALKGYRVAVIDCDSQASTTTIFGFNPDIDIDD
                     EETLLPFFRHGGEPDLKYALRSTAWPGIDLVPANLGLYQAEYEAAARLRGNPDALDRL
                     RRGVESMAGDYDVVLLDPPPALGMLSLAVLRAANALLIPTPPSTVDFASTAHFLRMIV
                     ETLEVMQNHLGARGYHFLRVVATKVDEGKSAHTQIRDMMEAVFGADMLSASLLDSAEI
                     DNANVQLRTVYELSGPTTKTYERCRNNLDRLNGEIELLIRKAWPSHRPELRRLGLG"
     gene            complement(2987..>3839)
                     /locus_tag="PLH31_02260"
     CDS             complement(2987..>3839)
                     /locus_tag="PLH31_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HQR88100.1"
                     /translation="KRKRLKPIEYTSPDGEVWVHVSANPAFGIATIWDADILIWATSR
                     INQMREQGVNDLPRTLHTTSYDLLKAIKRDTGGKGYKELQAALQRLEATTIQTSIRGS
                     KRNTKAQFGWLDEWVLESDPVTGAPKGLSLTLSNWVYEGVIKDRSLLTLHPDYFALAG
                     GVERAIYRIARKHAGDQPGGWTCRVSVLHDKTGSESPLKQFNYLLKRIVTANDLPEYD
                     LSFVNTADNSQAIHFVRRDAAERAAIKADLERLAADSARREREDARALQVDAMFEKRR
                     VPPSGDR"
     assembly_gap    3840..3849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4139..4498)
                     /gene="ssb"
                     /locus_tag="PLH31_02265"
     CDS             complement(4139..4498)
                     /gene="ssb"
                     /locus_tag="PLH31_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR88101.1"
                     /translation="MLNKVQLIGFTGADAEIRTVSGGKKVATLRVATNRYTKKDGDRK
                     DYTTWHQVEIWNQGTVGWLEGRHLPKGSKVFVEGEIRHDRYTDQSGQERFFSKVVVAT
                     PQHELKSLDRQEAADED"
BASE COUNT          876 a         1612 c         1704 g          897 t
ORIGIN      
        1 ggccgctggc gcgggaaagg cgaccagcgc gccgaccctc accggctcgg cctgtcgcag
       61 ataaagcccc tccggctcac tggccgttcg gttccacaag agtagcggac gagcgccccc
      121 ggccgtggcc atcgacagtg cgctgatgag cgcggccccg ccgcccagga cgcaggccaa
      181 cggcgtccaa cgcggggcag taggagtgag aaacatcagg tgggaccggc gcagccaaaa
      241 cgagccgaag gctcggcagg gcttgcggcc acgatgtggc taatgcccgg cgattgtggg
      301 cttagcgggc gtctagccgg tcactgatac cccgacaaac ggggcgagtt gcccatgggc
      361 aactagtcgg gaaatctcgt taggaggcga ggtccacgag ggtggaaggg agaagttgcc
      421 catgggcaac taggccttcg gacggacccg atcgagctcg cgggcgaagg cggcctggaa
      481 gtccgcgttg ctcgcctcgg cgtctagtga cagttcgagc acgacccgct tggcgccctt
      541 gcgcgccagg gtgaacaggg tcgccccagc gtcattggtc acgctcacga cgcccttggc
      601 ggcaggtttt ttggccgacg ccggcgccgc ggtcgccttg accagcaggg tcacgacctt
      661 ggcggggtca agcagcccct tcccgctcgc caccgccgcc gtctgaacgc cggccagacg
      721 cttggcttca gccatcacct tgggacgcga agcgtcgtcg gccagcaggg gtttcagcgt
      781 ccgggcatgg cgctccttca gctggcgcgg atcgccgaag gcttcgacga cgtcagtcgg
      841 cagcttgccg aggtccagga agcgcgagag ccacgctttg ggcacctcca gccgttcggc
      901 catgcgctgc gcgaccccgc cgtagaaggt ctcgaccgca tggcgataat cgagcgcgcg
      961 ctcgtagtcg ctgatgtcct ggcgggcgcg gttctcgacg tcggcgaggc gaaaggcctg
     1021 ctcgtcagtg agttcacgct cctcgatcag gtagcggatc tcgcgatgct cgacattgcg
     1081 caggtagctg accgaccaat gccggcgcgc gccactgatg acctcgtaat cgaaggccgg
     1141 atcgtcttcg acccgccgca cgatcgcggg aaactcctgc tcgccctggg cacgcaagct
     1201 ctctagcaga tcggcgcagg aggcctcgtt gagcaaatcg tagcgtcgat tatgacggct
     1261 ccacatccgg caacgggctg gatcaacgag cttgagggtc ttttccttga cgtccccgga
     1321 cgtcacccgc gccagcccgg tcaggcgggt gctcagacgc tccgttgcgg gcgcgccgcg
     1381 ttcaacggtc ggggtttcag tgaccggagc caccaagccc gcgaggatgg agcggttctt
     1441 gcctgttgtg gatgccatga ccgccctccc ctaccctagt ccaagccggc gaagctcggg
     1501 ccgatggctt ggccaggctt tgcgaataag cagttcgatc tcgccgttca ggcgatccaa
     1561 attgttgcgg cagcgctcat aggtcttggt cgtcggaccg ctcagctcat agacggtgcg
     1621 cagctggacg ttggcgttgt cgatctcggc ggagtcgagc agcgaggccg acagcatgtc
     1681 ggcgccgaac accgcttcca tcatgtcccg gatttgcgtg tgggcgctct tgccctcatc
     1741 gaccttggtc gcgacgacgc gcaggaaatg gtagccgcgg gcgcccaggt ggttctgcat
     1801 cacctcgagc gtctcaacga tcatccgcag gaagtgcgcc gtcgaggcga agtcgacggt
     1861 cgagggcggc gtcggaatca gcagcgcatt ggcggcgcgc agcacggcca gcgaaagcat
     1921 acccagcgct ggcggcggat ccagtaggac cacgtcgtag tccccggcca tactttccac
     1981 gccgcgacgc agacggtcga gcgcgtccgg attgccgcga agccgtgccg ccgcctcgta
     2041 ttcggcctgg tataacccaa ggttcgcagg caccagatcg atgcccggcc aggcggtaga
     2101 ccgaagggca tacttcaggt ccggttcgcc accatggcgg aagaagggga ggagggtctc
     2161 ctcgtcgtcg atgtcgatgt ctgggttgaa gccgaagatc gtcgtggtac tcgcctggct
     2221 gtcacagtcg atcaccgcca cccgatagcc cttcagagcc agaaactggg cgacgtggca
     2281 ggtcagcgtg ctcttgccga ccccaccctt gaagttctgc accgccagga cgatgggtgg
     2341 atcgtcaggc ccacgccggg ggcgtgtgcc gaagacgtcg cgcatgtgat tgacctcgga
     2401 caggctgtag ccttcccggc ggccggtcgc gctcaggcga ggggccggga ggcgtccgtc
     2461 ggcttcggcc tggcgaatgg cttctgaagt ccgcccgacc atctcggccg ccttggtgac
     2521 cgtgaaccgc aggtcgacga tcttctgctc acccggcgca aacaccgtgg cgcgaaggcg
     2581 ggtgatgacg tcatgcgccc ggtcgctgag cgccgacatg gcggcgagca ggaccgggcc
     2641 ggaagcggtg ctttcagcta gggcttgctt cgccatggtc cgcctccttt gcaaatatcc
     2701 gacatcatgt ctggaatttg ccaagttttc ggtcggatac aatgaagaaa gggttaacag
     2761 cgcgccgagc ctgaaccctc ttggttcaag gcgacggcaa gccagcctgt ccgccacacc
     2821 ccgttagaag atatcttagg gataaggtta gacagagtta ggcgccgaac cgaagccgga
     2881 aaagccagtc cctacaaggg gttttcagat cggccggtca ccgaaacccc gaggagaggg
     2941 tcaccgatcc cccggcaaga cggtcatcga tcccccgtgg aaaaggtcac cgatcccccg
     3001 agggcggaac cctacgcttt tcaaacatcg catcgacctg caaggcccga gcgtcctcgc
     3061 gctcacggcg ggccgaatcg gcggcgagtc gctcgagatc ggccttgatg gcggcgcgct
     3121 cggcggcgtc gcgccgcacg aaatggatgg cctgggaatt gtcggcggta ttgacgaagc
     3181 tgaggtcgta ttccggcaga tcgttggcgg tgacgatccg cttgagcagg tagttgaact
     3241 gcttgagcgg gctctcgctc ccggtcttgt cgtgcagcac gctcacccga caggtccagc
     3301 cgccgggttg gtcgccggcg tgcttgcggg cgatccgata gatggcccgc tcgaccccgc
     3361 cggccagtgc gaagtagtcc ggatgcaggg tcagcaggga gcgatccttg atcaccccct
     3421 catagaccca gttggacagg gtcaggctga gccctttggg cgcgccggtg accggatccg
     3481 attccagcac ccactcgtca agccagccga actgcgcctt ggtgttgcgc ttcgagccgc
     3541 ggatcgaggt ctggatcgtg gtggcttcga gacgctgtag cgccgcctgc agctccttat
     3601 agcccttgcc gccggtgtcg cgcttgatcg ccttcaacag gtcatagctg gtggtgtgca
     3661 gggtgcgcgg cagatcgttg acgccctgct cgcgcatctg attgatccgt gaggtcgccc
     3721 agatgaggat gtcggcgtcc cagatggttg cgatgccaaa tgccgggttg gcgctgacat
     3781 gcacccagac ctcgccgtcg ggactggtgt attcgatggg cttcagccgc ttgcgtttgn
     3841 nnnnnnnnnc gaaaagaacg gccgttccat cacctcccgc tggtctttca gcggcaggct
     3901 gctcatccga gggatgaaga gatcgaactc ctgttgtcga atcgcgcgct tggacatctt
     3961 gggcgccacc atgctcattt gtgagaggga agggtagggg agggcgcgcc aaggcgacct
     4021 cggaccgaaa agttgcccat gggcaacttc acccggaacc accctataaa cgcccttcct
     4081 ttgcggcctt tgcggccttt gcgggcccgc ccggagaccg gacgggccct gccgaaggtt
     4141 agtcctcgtc ggccgcctcc tgacgatcga gggacttcag ctcgtgctgc ggcgtggcga
     4201 cgaccacctt ggagaagaag cgctcctgac cgctctggtc ggtgtagcgg tcgtggcgga
     4261 tctcgccttc gacgaacacc ttggagccct tgggaagatg gcggccttcc agccagccga
     4321 ccgtgccttg gttccagatc tccacctggt gccaggtggt gtagtctttc cgatcgccgt
     4381 ccttcttggt gtagcggttg gtggcgaccc gcagggtggc gaccttcttg ccgcccgaga
     4441 cagtgcggat ttcagcgtcg gcgccggtga agccgatgag ttgaaccttg ttgagcatca
     4501 cgtttctcca atcgaccgcc gggaaaagcc ccggcggctc gcggtccctg ccgcgatcac
     4561 gcgacctgca gggccgggca aaggggccgg gccaaagccc gcggggggtg gaaaccggcc
     4621 tgcacagagc gcagcggcgg aaggccggag ggtccgcacc ccgcgcctcg cgcgcgaggt
     4681 cggctttgac ctggaaccgc ccggtctagg ttcgtgggat tgatcgcggg ggaccgcagc
     4741 ccgctggacg tttcctcggg gtattggtga ccggcctcaa cggtgaactg gcttcatgcg
     4801 ccaggccaga tttccacgcc gcgggcttaa gaggtcgcca gcagggcgtc attgccgccg
     4861 ccaaggacgg ctcggaccac taccctagga aggcgactgg tctggaccac ggaccgtaac
     4921 tgctaggaaa ccggcgaacc cgagggcaga agaccgtcct ggagcgatcc ccgtccgaca
     4981 tccgccaagg tcagatgctg cggctcgcgg tcggcggtcc acgaggggta agggtagaaa
     5041 ctccgccagg cggatcgaca ggcgcgccca gttgagtttt gatccgctcg aaggcgatc
//
LOCUS       DAOMEO010000060         5097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2075_length_5097_cov_2.499802, whole genome shotgun sequence.
ACCESSION   DAOMEO010000060 DAOMEO010000000
VERSION     DAOMEO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_5097_cov_2.499802"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLH31_02270"
     CDS             complement(<1..481)
                     /locus_tag="PLH31_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR88102.1"
                     /translation="MDRLILMRHGQAERQAATGGDFERGLTPKGREDATLMGRLLAKS
                     GATPDLVLVSSARRTRETFAAVSAAFPKARVEFRRDLYHAEPEEVVTALEDEGDSASV
                     VMVVGHNPGLHELALRLILQGGAEPVALNKVRSRFPTATTAVFALGAGDPPVLEHLFY
                     "
     gene            560..1714
                     /locus_tag="PLH31_02275"
     CDS             560..1714
                     /locus_tag="PLH31_02275"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQR88103.1"
                     /translation="MADLDLAPAALSDGGTWRFPASEPLRLDSGGVIEGLEVAYQTYG
                     QLNADRSNAVLICHALTGDQHVASPHPVTGKPGWWLRLVGPGKPLDPTRHFIICSNVV
                     GGCMGSTGPASINPATGKPWALSFPVITIADMVRAQAMLVKALGVETLFAVVGGSMGG
                     MQVQQWAVDYPERLASAVIIASASRHSAQNIAFHEVGRQAIMADPDWRGGAYADLGAR
                     PEKGLAVARMAAHITYLSEPALQRKFGRELQRDGLSWGFDADFQVESYLRHQGASFVD
                     RFDANSYLYITRAMDYFDIAASHGGVLANAFTRARHMRFCVLSFTSDWLYPTAENRHI
                     VRALTAAGCRVAFVEIESDKGHDAFLLDEPVMDAALTGFLGSVERERGLA"
     gene            1711..2370
                     /gene="metW"
                     /locus_tag="PLH31_02280"
     CDS             1711..2370
                     /gene="metW"
                     /locus_tag="PLH31_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HQR88104.1"
                     /translation="MSLVREDFREILRLVRPGSRVLDVGCGEGALLDLLIHDKQVDGR
                     GLEISAGGVAACMARGLSVVQGDADQDLDHFPDKTFDYAILSQTLQATRQPRHVLDEL
                     LRIADRAIVSFPNFGHWRVRWSLLSRGRMPETKALPMPWWSTPNIHLCTLVDFLALTD
                     DLDVRVDACAAFAGAGPARHIDPASPIENWRAETCLFMLSRGSAPAREPPRVSGDLFD
                     A"
     gene            2367..3164
                     /locus_tag="PLH31_02285"
     CDS             2367..3164
                     /locus_tag="PLH31_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR88105.1"
                     /translation="MKLRLMTYNVHRCVGTDRRLDVERVAEVIAAEMPDVVALQELDV
                     LRRRTRTIDQAHRLAELLKMKFHFHPAMAVEEELYGDAILTALPERRIKAAGLPLYRR
                     VPGLEPRGALWVEVMVGDTPVQIINTHLGLVPQEQKRQAAALLGEKWMASDQWTAPGV
                     LLGDFNASPYSGTYRMLRAAVRDAQTPGAGWGRAPTATFPSSFPFMRIDHVFLTKGLE
                     TIAVRSPYDARARVASDHLPLVVDLEVTAAGDAAGPAAGPSGEPDPA"
     gene            complement(3100..4599)
                     /locus_tag="PLH31_02290"
     CDS             complement(3100..4599)
                     /locus_tag="PLH31_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQR88106.1"
                     /translation="MGERSSVFWPGHNCWRVEPADRLALFIDNDEAFDALKHLLLSAR
                     RSIWILAWVFDPLTRLDPDRVKRSRDPTRADRIGLLLRRQAALNPALDVRILTWDMPF
                     PIAAAQLFGPQRGAAFFAGSRVKYQLDNTLPASACHHQKVVVIDGAAALLSGGDIGVD
                     RWDTCEHLDNDPRRRLPTGRRYPPRHEVAMLVDGRAAEALGELFAARWRNATGEALAP
                     PERPEDIPWPPGLLPDLRRAQVAISRTEAAWKGRPEVAESLQLHLAGIRAAKRLIYLE
                     NQYLTSPIIVEALAERLAEPDGPEVVTVGPARSPSYFDQLTMDSARTSAINRLRSIDA
                     YNRFSAFSAHTPKGGPIIVHSKVAIIDDRMLRVGSANLNNRAIGLDTECDLAVEGRDP
                     LEREVITAFLARLIGHFLADVIEAMQREGGLAAAIHALDVKGGPPRRLQPVPVRHLTG
                     VQQFIADWSLGDAISPDDAWRPWGRRERLKRDLVRLTAPPPALPHHLQP"
     gene            complement(4741..5021)
                     /locus_tag="PLH31_02295"
                     /pseudo
     CDS             complement(4741..5021)
                     /locus_tag="PLH31_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
BASE COUNT          777 a         1759 c         1752 g          809 t
ORIGIN      
        1 cgtagaagag atgctccagc acgggcgggt cgcccgcgcc cagcgcaaag accgccgtgg
       61 tggcggtggg aaagcggctg cggaccttat tgagcgcgac cggctcggcc ccgccctgca
      121 ggatcagccg cagggccagt tcgtgcaggc cgggattgtg gccgaccacc atcacgacgc
      181 tggcgctgtc gccctcgtcc tccagggccg tgaccacctc ttcgggctcg gcgtgataga
      241 ggtcgcggcg gaactcgacc cgggccttgg gaaaggcggc cgagacggcg gcgaaggtct
      301 cgcgggtgcg gcgggcgctg gagaccagca ccagatcggg cgtcgcgccg cttttggcca
      361 gcaaccggcc catcagagtc gcgtcctcgc gccctttcgg ggtcaagcca cgctcgaaat
      421 cgccgcccgt ggcggcctgc cgttcggcct ggccgtggcg catgaggatc agacggtcca
      481 tgcgccctcc atagcctcac ccggggcccg ccggaagcgg gcggttgaca ccttcttcgc
      541 caagcggtcc aaggcggtca tggctgacct ggatttggcc ccggccgcgc tgtccgacgg
      601 cggaacctgg cggttcccgg cgagcgagcc cctgcgcctc gactcgggcg gcgtcatcga
      661 ggggctcgag gtcgcctacc agacctatgg ccagctgaac gccgaccgct ccaacgccgt
      721 gctgatctgc cacgccctga ccggcgacca gcatgtagcc tcgcctcatc cggtgaccgg
      781 caagcccggc tggtggctgc ggctggtggg acctggcaag ccgctggacc cgacgcggca
      841 tttcatcatc tgcagcaatg tggtcggcgg ctgcatgggc tcgacgggcc cggcctcgat
      901 caaccccgcc accggcaagc cgtgggccct gtcgttcccg gtgatcacca tcgccgacat
      961 ggtccgcgcc caggcgatgc tggtgaaggc cctgggcgtc gagaccctgt ttgccgtggt
     1021 cggcggctcg atgggcggga tgcaggtgca gcaatgggcg gtggactatc ccgagcggct
     1081 ggccagcgcg gtgatcatcg cctcggcctc gcggcactcg gcccagaaca tcgccttcca
     1141 cgaggtgggg cgccaggcga tcatggccga tcccgactgg cgcggcggcg cctatgccga
     1201 ccttggcgcc cggcccgaaa agggcctggc cgtggcgcgg atggccgccc acatcaccta
     1261 tctgtcggaa ccggccctgc agcggaagtt tggccgcgaa ctgcagcgcg acggcctgtc
     1321 ctggggcttt gacgccgact tccaggtgga gagctatctg cgccaccagg gggccagttt
     1381 cgtcgaccgc ttcgacgcca acagctatct ctacatcacc cgggcgatgg actatttcga
     1441 catcgccgcc agccatggcg gggtgctggc caacgccttc acccgggcgc ggcacatgcg
     1501 cttctgcgtg ctgagtttca ccagcgactg gctgtatccc accgccgaga accgccacat
     1561 cgtgcgggcc ctcacggcgg ccggctgccg cgtggccttc gtcgagatcg agagcgacaa
     1621 gggccacgac gccttcctgc tggacgagcc ggtgatggac gccgccctga ccggcttcct
     1681 gggctcggtt gaacgcgaac gggggctggc gtgagtctgg tgcgcgaaga cttccgcgaa
     1741 atcctgcgcc tggtgcgccc cggctctcgc gtgctggacg tcgggtgcgg cgaaggcgcc
     1801 ctgctggacc tgctgatcca tgacaagcag gtggatggcc ggggcctgga gatcagcgcc
     1861 gggggcgtcg ccgcctgcat ggcgcgcggc ctgtcggtgg tgcagggcga cgccgaccag
     1921 gatctggacc acttccccga caagaccttc gactacgcca tcctgtccca gaccctgcag
     1981 gccacacgcc agccgcgcca tgtgctggac gagttgctgc ggatcgccga ccgggccatc
     2041 gtctcgttcc ccaatttcgg ccactggcgg gtgcgctggt cgctgctgag ccggggccgg
     2101 atgcccgaga ccaaggccct gcccatgccc tggtggtcga cgcccaacat ccacctgtgc
     2161 accctggtcg acttcctggc cctgaccgat gacctggacg tgcgcgtcga cgcctgcgcg
     2221 gccttcgcgg gcgccgggcc ggcccgacac atcgatccgg ccagcccgat cgagaactgg
     2281 cgggccgaga cgtgcctgtt catgctgagc cggggaagcg cgcctgcgag ggaacctccg
     2341 cgggtttcag gcgatctctt cgacgcatga agcttcgcct gatgacctat aacgtccacc
     2401 gctgtgtggg cacggaccgc aggctcgatg tcgagcgcgt ggccgaggtg attgcggctg
     2461 aaatgccgga cgtggtggcg ctgcaggaac tggatgtcct gcgtcggcgc accaggacca
     2521 tcgatcaggc ccaccgtctg gcggaactgc tgaagatgaa gttccacttc catccggcca
     2581 tggctgtgga agaagagctg tatggcgacg cgatcctcac cgccctgccc gagcgccgga
     2641 tcaaggccgc aggcctgccg ctgtatcgcc gggtgccagg ccttgaaccg cgcggcgccc
     2701 tgtgggtcga ggtcatggtc ggcgacacgc cggtccagat catcaacacc catctgggtc
     2761 tggtgccgca ggaacagaag cgccaggccg ccgccctgct gggcgaaaaa tggatggcct
     2821 ccgaccagtg gaccgccccc ggcgttctgc tgggcgactt caacgcctca ccctattcgg
     2881 gcacgtaccg gatgctgcgc gccgccgtgc gcgacgctca gacgccgggg gccggctggg
     2941 gccgggcgcc caccgcgacc tttccgtcca gctttccgtt catgcgcatc gaccatgtct
     3001 tcctgaccaa gggcctggag accatcgccg tgcgttcgcc ctatgacgcc cgggcgcgcg
     3061 tggcctccga ccacctgccg ctggtggtcg atctggaggt cacggctgca ggtgatgcgg
     3121 cagggccggc ggcggggccg tcaggcgaac cagatcccgc ttgagacgct cgcggcggcc
     3181 ccagggccgc caggcgtcgt cgggcgagat ggcgtcgccc aggctccagt cggcgatgaa
     3241 ctgttgaacg cccgtgaggt ggcgcaccgg caccggttgc agccggcgcg gcgggccgcc
     3301 cttgacgtcc agggcgtgga tcgccgccgc cagcccgccc tcacgctgca tggcctcgat
     3361 cacgtccgcc aggaaatgcc cgatcagccg cgccaggaag gcggtaatca cctcgcgttc
     3421 cagcgggtcg cgcccctcga cggccaggtc gcactcggtg tcgagaccga tggcgcggtt
     3481 attgagattg gccgagccca cccgcagcat ccggtcgtcg atgatggcca ccttggagtg
     3541 gacgatgatc ggcccgccct tgggcgtatg ggcgctaaag gccgaaaagc gattataggc
     3601 gtcgatcgac cgcagccggt tgatggccga ggtgcgcgcg ctgtccatgg tcaactgatc
     3661 gaaatagctg gggctgcggg ccggtcccac cgtcaccacc tcgggtccgt cgggctcggc
     3721 cagacgctcg gccagggcct cgacgatgat cggcgaggtc agatactggt tttccagata
     3781 gatcagccgc ttagccgccc ggatcccggc cagatgcagc tgcaggctct cggcgacctc
     3841 ggggcggccc ttccaggccg cttcggtccg gctgatcgcc acctgggcgc ggcgcaggtc
     3901 gggcaagagc cccggcggcc aggggatgtc ctcgggtcgt tcgggcgggg ccagcgcttc
     3961 gccggtggcg ttgcgccagc gggcggcgaa cagttcgccc agagcctcgg ccgcccggcc
     4021 gtcaaccagc atcgccacct cgtgccgcgg gggatagcga cgtcccgtcg gcaggcggcg
     4081 gcggggatca ttgtccagat gctcgcaggt atcccagcga tcgacgccga tatcgccgcc
     4141 gctcagcagg gccgcggcgc cgtcgatgac caccaccttc tggtgatggc aggccgaggc
     4201 cggcagggtg ttgtccagct gatacttgac ccgcgacccc gcgaagaacg ccgccccgcg
     4261 ctgcggccca aacagctggg cggcggcgat cggaaagggc atgtcccagg tcaggatccg
     4321 cacgtccaga gccggattca gggccgcctg acggcgcagc agcagcccga tgcggtcggc
     4381 gcgcgtcggg tcgcggctgc gcttgacccg gtcaggatcc aggcgggtca gggggtcgaa
     4441 cacccaggcc aggatccaga tcgaacgccg ggccgacagc agcagatgct tgagggcgtc
     4501 gaaggcctcg tcattgtcga tgaacagggc cagcctgtcg gccggctcga cgcgccagca
     4561 gttgtgacct ggccagaaaa cagaagaacg ctcgcccatg acgctccaat cgcggtgaat
     4621 ttcgacaacg cgcggacccg gattatggct ccgcttcaaa tgcgtttcga agacatcgcc
     4681 gtcacaggcg gtttcgcccg accctgatga aacaatcagg tcgcaaaggg ctctggcaat
     4741 ctagccggcg atggcggccg cgtcacgttc gccggtgcgg atgcgcaggg cctgttccag
     4801 gtccatgacg aacaccttgc cgtcgccgat cttgccggtg ttggccgtgc gctgcagcgc
     4861 ttcacctcgt attcggcgcc gcgatagacc tcggtcttgc ccttctgccg gccatagccc
     4921 cgcacctcgg tcaccgtcag tcctgacgcg ccggcctcgg tgaccgcttc gagaacggcg
     4981 tcaaggcggc tcggcttgat gatggcgacg atcattttca tggcgcggct cccgcagcga
     5041 atagtgatta cgaccctaag ctaggctccg aacgcggcgc gtccaagtgc gatccgt
//
LOCUS       DAOMEO010000061         5093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2078_length_5093_cov_2.695316, whole genome shotgun sequence.
ACCESSION   DAOMEO010000061 DAOMEO010000000
VERSION     DAOMEO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2078_length_5093_cov_2.695316"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLH31_02300"
     CDS             <1..904
                     /locus_tag="PLH31_02300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR88107.1"
                     /translation="PTQAWDALICTSPSVQSAMTSMFDEWTAYLADRFGGKAQPRPQL
                     PLIPLGVNAAALGGAADRPEVRAATRENLAIGTDDILILWVGRLSFFEKAFPQAMMRA
                     AEEAAQATGKRVHFAMVGWFPNGADGERMYRQAVEAHAPSIVFHVIDGNDRQRLATLW
                     SAADVFISLVDNIQETFGITPLEAMASGLPVVVSDWDGYRYTVRDGEEGFLIPTLGGP
                     EEGFVGDLVGEHALGFKSYQQYVGIVAQHTAVHVGRAVEALTQLIQSPDLRRRMCARD
                     VRLVRGRTAIYGPGRGASRDPTCL"
     gene            843..1244
                     /locus_tag="PLH31_02305"
     CDS             843..1244
                     /locus_tag="PLH31_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88108.1"
                     /translation="MVAPQYTALAAELAAIRHASDDPRALSRAHPVKGDPFRDFAGFA
                     TSTLNLETRLRLRQGVTAEAVERVRTIELDMFAANWRGTLEEALRVIQLLSNDAVLTV
                     RELLTAFPTPRRRHVQMSLVWMAKQGFLDWL"
     gene            complement(1251..1610)
                     /locus_tag="PLH31_02310"
     CDS             complement(1251..1610)
                     /locus_tag="PLH31_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88109.1"
                     /translation="MPLKDMLLCLGFSLALPAGQMLFKWGADYSRGIQGNFIAKVIFN
                     YPLMGAFAWYGLTALFWFYILTRVPLSLAYPFSILGAGLVPILAWVLFRETITPQAWL
                     GYVIMLGGFLLIARSGA"
     gene            complement(1616..2626)
                     /locus_tag="PLH31_02315"
     CDS             complement(1616..2626)
                     /locus_tag="PLH31_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR88110.1"
                     /translation="MISVAQDRADRSAPARIAAILPCYRSSAHVLDVIAAIGPEVEFI
                     VCVDDACPDKVGDHIEAGTKDTRVVVLRHAANQGVGGAVCTGYAHALDLGADILVKID
                     SDGQHDPAEILALADPIARGDADYTKGNRFFNIEDLQGMPTIRLLGNAGLSFLTKLSS
                     GYWSVFDPTNGYTAVQARVIERVPLDKLAKRYFFESDLLFRLGTLRARVVDVPMTAIY
                     ADEVSQMQVGKIVLPFLAANIVNFHKRLFYNYVLRNFSVASLYFIGGGPLALIGMIRG
                     VRAWADGVADARPATSGQVMLAALPILVGVQMLLSFVAYDVEAEPRTLLRSKRLLAPK
                     KA"
     gene            complement(2623..3327)
                     /locus_tag="PLH31_02320"
     CDS             complement(2623..3327)
                     /locus_tag="PLH31_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88111.1"
                     /translation="MSPRSFALTLSTFALILTACDQPKGAPRVDDPAPPSLAQAPAAA
                     AAPAAPKPPRPPEPIVWDEVSERFTLDGMVVKTAHLWRFDRSLEAFSSLGSRLQPAPG
                     GGLQVIEDAPDAVLRSPQNLAIDGKRYNAVLVRLTRVQPSEKWDGSIFYTTKAHGEAA
                     AFHTKPLRGANPVQGETTIMVYDMANLKKGGGDWTASTISGLRLDFDDSVGGGFLVHQ
                     IAVVSLPPAAMLRPSQ"
     gene            complement(3324..4730)
                     /locus_tag="PLH31_02325"
     CDS             complement(3324..4730)
                     /locus_tag="PLH31_02325"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HQR88112.1"
                     /translation="MRKLEIPGLGLTFTASGLVRAALILGLLFMLGASLPGQMSYDSL
                     AQLHEGRFAVRETWGPAIYGWLLGVFDEIVPGTGLYVVASALLLFLSLSSLRQLRPDI
                     SWWAPPVTVLLVFSPLVLIYQGVVWKDVLFANLAIASFACLAHAPRFWDRPGPRWLVL
                     IGAMVLLATAALVRQNGLVAVLMAAIVLGVLRRREGWGRALTWAVGGLVATLVLAQVL
                     AVIAQPKSAGEDKAGGIGVRIVQHYDLIGAMAHDPTYRLSRIEQANPAAAAAMRRGVA
                     VYSPERVDFFDRDPTLGPNIWPMPNELVGAEWRDLITKHPKTYLAQRADVFRWLFLTP
                     KLERCLPVFVGVEGPEPLVSSLKLTSGRDPADVSLANYATYFYGTPVFSHLAYALIAL
                     AVTVFLLWRRDEADYAIAGLMLSALGFTASFFVISIACDYRYLYFLDLAAMAGLFYLA
                     LDPSLRRSSDTDPRSIPA"
     gene            complement(4808..>5093)
                     /locus_tag="PLH31_02330"
     CDS             complement(4808..>5093)
                     /locus_tag="PLH31_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88113.1"
                     /translation="KAEREAGMAQALSENLQAAITHYQGVAEQWKREALAEQVKVEIL
                     RDRVAKAEREAGMAQALSENLQAQNAAILSSTSWRVTWPLRAIVRRLRPG"
BASE COUNT         1048 a         1603 c         1598 g          844 t
ORIGIN      
        1 gccaacccag gcctgggacg ccttgatctg cacctccccc tcagtccaga gcgctatgac
       61 gtccatgttc gacgaatgga cagcctatct cgccgatcgg ttcggcggaa aggctcaacc
      121 tcgaccgcaa ttgccgctga ttcctctggg cgtgaacgct gcggcccttg gcggcgccgc
      181 cgacaggccc gaggtgcggg cggcgacccg cgaaaatctg gcgatcggga ctgacgatat
      241 tctcattttg tgggtcggcc gcttatcgtt cttcgagaag gcctttcccc aggcgatgat
      301 gcgggcggct gaggaagcgg cgcaggcgac cggcaagcgc gttcatttcg ccatggtcgg
      361 ctggttcccg aacggcgccg atggcgaacg catgtatcgg caggcggtcg aggctcatgc
      421 cccctcgatc gtcttccacg tcatcgacgg caatgaccgg caacgcctgg ctaccctgtg
      481 gtcggccgca gacgtcttca tttcactagt ggacaacatc caggagacct tcgggatcac
      541 ccccctagag gccatggcct ccggcctgcc cgtcgtcgtc agcgactggg acggctatcg
      601 ctacaccgtt agagacggcg aagaggggtt cctgatccca accctagggg gacctgaaga
      661 gggctttgtc ggcgatctgg tcggcgaaca cgctttgggt ttcaaatcct atcagcagta
      721 tgtcggcatt gtcgcccaac ataccgctgt ccatgtgggt cgggcagttg aggccctgac
      781 ccagcttatt caatcgccag atctgcgacg ccgaatgtgt gcgcgagacg ttcgactggt
      841 ccgtggtcgc accgcaatat acggccctgg ccgcggagct agccgcgatc cgacatgcct
      901 ctgatgaccc aagagcgctt tcgcgcgcgc acccggtcaa gggggatccg ttccgcgact
      961 tcgccggttt cgccacctcg accctgaacc tcgaaacccg gctgcgtctt cgccaaggtg
     1021 taacagccga agccgtggaa cgcgtccgga ccatcgagct ggatatgttt gccgcgaact
     1081 ggcggggaac ccttgaagaa gcgctcaggg tcatacagtt gctgagcaat gatgcggtgt
     1141 tgacggttcg ggagctgctg accgccttcc cgacgccccg gcgtcgccat gtccagatga
     1201 gtctcgtctg gatggccaag caaggcttcc tggattggct atagccaatc ctaggcgccg
     1261 ctccgcgcaa tcagcaggaa accgcccagc atgatcacat agcccagcca ggcctgaggg
     1321 gtgatggtct cgcgaaacag gacccaggcc aggatcggca ccaggccggc gccaagaatc
     1381 gaaaagggat aggccagact caagggcacc cgggtcagga tgtagaacca gaacagggct
     1441 gtgagcccgt accaggcgaa ggcgcccatc agcggatagt tgaagatcac cttggcgatg
     1501 aaattccctt ggatcccccg actgtagtcg gcgccccact tgaacagcat ctggcccgcc
     1561 ggaagggcga gggaaaagcc caggcaaagc agcatgtcct tgagcggcat ggcgtctagg
     1621 ccttcttggg cgcgagcagg cgctttgagc gaagcagggt cctgggctcg gcctcaacgt
     1681 cataggccac aaagctcaat agcatttgta cgcccaccag gatcggcaag gccgccagca
     1741 tcacctggcc tgatgtcgcc ggccgcgcgt cggcgacgcc atcggcccag gcgcgcacac
     1801 cgcggatcat gccgatcagg gccaacggcc ctccgccgat gaaatagagt gaagccaccg
     1861 agaaattgcg caggacgtag ttgtagaaca gacgcttgtg gaagttgacg atattggccg
     1921 ccaggaatgg cagaacaatc ttgcccacct gcatctggct gacttcgtcg gcgtagatcg
     1981 ccgtcatcgg cacgtcgact acgcgggccc gaagggtgcc cagccggaac aacaggtctg
     2041 actcgaagaa atagcgcttg gccagtttgt ccaacggcac ccgctcgatc acgcgggcct
     2101 ggacagcggt atagccgttg gtcggatcga atacgctcca atagcccgaa gacagcttgg
     2161 tcagaaacga caggccagca ttgccgagca gccgtatggt cggcatgccc tgcaggtcct
     2221 cgatgttgaa gaagcgattg cccttggtgt agtcggcgtc tccccgcgcg atgggatcgg
     2281 ccagggccag gatctcggcg ggatcgtgct gtccatcgct atcgatcttc accaggatgt
     2341 cggcgccaag gtccagggcg tgggcgtaac cggtacacac cgcgccgccg acgccttggt
     2401 tggccgcatg tcgcaacacg acaacccgcg tatctttcgt tccggcctcg atgtggtcgc
     2461 cgaccttatc agggcaggcg tcatcgacgc acacgatgaa ctcgacctcg ggcccgattg
     2521 ccgcgatcac gtcaagaaca tgagcgctgg atcgatagca cggcaagatc gccgcgatcc
     2581 tggcgggggc tgagcggtcc gcgcgatctt gggcgacaga gatcattgcg acggtcgcag
     2641 catcgccgcg ggcggcaagg acacgacggc gatctggtgg acaagaaagc cgcccccgac
     2701 actatcgtcg aaatccagac gcaggcccga aatggtcgac gccgtccagt ctcccccgcc
     2761 cttcttcaga ttggccatgt catagaccat gatcgtcgtc tcgccctgaa cggggttggc
     2821 gccccgcaag ggcttggtgt gaaacgccgc cgcttcgcca tgcgccttgg tcgtgtagaa
     2881 aatcgatccg tcccactttt cggaaggctg cacacgcgtc agccgcacca gaacggcgtt
     2941 gtagcgtttg ccatcgatgg ccagattctg aggcgagcgt aacaccgcgt caggagcatc
     3001 ttcgatcacc tggaggccgc cacccggcgc cggctgaagc ctggagccga gtgaagaaaa
     3061 cgcctccagg cttcggtcga aacgccaaag atgggcggtc ttcaccacca tgccatccag
     3121 tgtgaagcgt tcgctgacct cgtcccagac aatcggttcc ggtgggcgcg gcggcttggg
     3181 agccgcgggc gccgccgccg cggcgggagc ttgagccagc gacggcgggg cggggtcatc
     3241 gacgcgtggg gcgcccttgg gttggtcgca ggccgtcagg atcaaggcga acgtcgacag
     3301 ggtcagggcg aaagaacgag ggctcaagcc ggaatgctcc gtgggtcggt gtcgctcgaa
     3361 cgccgaagcg agggatcgag ggcgagataa aacaagcccg ccatggcggc cagatccaga
     3421 aaatatagat agcgatagtc acaggcgatc gagatcacga agaaactggc cgtaaatcca
     3481 agggccgaca gcatgagtcc cgcgatcgca tagtcggcct cgtcgcgccg ccacagcaga
     3541 aacacggtca cggccaaggc gatcagggca taggccagat gggagaacac gggggtgccg
     3601 taaaaatagg tcgcgtagtt ggccagggag acatcagccg gatcccggcc gctggttagc
     3661 ttcagggagg acaccagggg ctcagggccc tcgacgccga caaagaccgg caaacaacgc
     3721 tccagcttgg gcgtcagaaa caaccaacgg aacacgtcag cgcgttgcgc gagataagtc
     3781 ttgggatgtt tcgtgatcag gtcgcgccac tcggcgccca ccagctcgtt gggcatgggc
     3841 cagatgttcg gacctagggt gggatctcgg tcaaaaaaat cgacccgttc cggcgaatag
     3901 accgccaccc cacgccgcat ggcggcggcg gcggcgggat tggcttgctc gatacggctg
     3961 agacgatagg tcggatcgtg cgccatggcg ccgatcaggt cgtaatgctg cacaatccgc
     4021 acgccgatgc cgccggcctt gtcctcgccc gcggattttg gctgggcgat gacggccagc
     4081 acctgagcca gaaccagggt cgcgaccagg cctcccacgg cccaggtgag cgcccggccc
     4141 catccctcac gacgacgcaa gacgcccagg acgatcgccg ccatcagcac ggccaccagg
     4201 ccgttttgac gaaccagcgc cgctgtcgcc aacagcacca tcgccccgat cagaaccaac
     4261 caacgggggc caggacgatc ccagaaacgt ggagcatggg ccagacacgc gaagctggcg
     4321 atggccagat tggcgaagag cacgtccttc cagacgacgc cctgatagat cagcacaaga
     4381 ggtgagaaga ccagcagcac cgtcaccggc ggcgcccacc aggagatgtc cggccgaagc
     4441 tggcgaagcg aggacaggct taaaaacagc agaagggcgc tggctacaac ataaaggccc
     4501 gtgccaggga caatctcgtc aaagacgccc agcagccagc catagatcgc tgggccccag
     4561 gtttcgcgta ccgcgaagcg gccttcatgc agttgcgcca ggctgtcata ggacatctgg
     4621 cccggcaggc tggcgccaag catgaacaat aagccgagga tcagcgccgc ccgcaccagt
     4681 cccgacgccg tgaaggtcag acccaggccg ggaatctcca gctttcgcat tggcgagtcg
     4741 ccccttccgt ttagtcctag atatcgtccg ccaaggctta gacgagaaaa aacgtctcgg
     4801 ccgccgatca gcccggacgc aggcgacgca cgatagcccg caggggccag gtcacgcgcc
     4861 aactggtgct ggacagtatg gcggcgtttt gagcctgcag attttcgcta agcgcctggg
     4921 ccataccggc ttcacgctcg gcttttgcga cccggtcacg caaaatctcg accttgacct
     4981 gttcagccag ggcctcacgt ttccactgct cggcgacgcc ctggtagtgg gtgatcgcag
     5041 cctgcagatt ttcgctaagc gcctgggcca taccggcttc acgctcggct ttt
//
LOCUS       DAOMEO010000062         5090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2080_length_5090_cov_2.408540, whole genome shotgun sequence.
ACCESSION   DAOMEO010000062 DAOMEO010000000
VERSION     DAOMEO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_5090_cov_2.408540"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLH31_02335"
     CDS             <1..866
                     /locus_tag="PLH31_02335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR88114.1"
                     /translation="LSGFVLAHAYGARTMGWLGFMRARLIRVWPLIALSMVAGALVMI
                     GHRDNILICLLMGLLVFPRIWVSDEDSFSPLFPLNPPAWSLFLELLASALWFPARRLS
                     TMWLVLVIVVSGGVLIAIAYGMGGVQTGWDRATYWIGIIRTIFPFALGWACYRIQAYV
                     TVSLPAWILALVLLAVLAVPPMDYTATYDLVCVAIVFPLIVLLGHRDPTGRVGAICRF
                     SGEISYPLYALHWVLWELSLRAFRAMGGKGYPDVLVVTAILLIIAGAWGVLKVYDLPV
                     RRWLRARLVRD"
     gene            complement(927..2012)
                     /locus_tag="PLH31_02340"
     CDS             complement(927..2012)
                     /locus_tag="PLH31_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQR88115.1"
                     /translation="MAVPSHMNKVALRGRGARTNDTSRFETFRSEAFEDGWTEADEAA
                     RPLTTTIQPMKSKTIIARNDSPDISFSRSINPYRGCEHGCIYCYARPAHAYLGLSPGL
                     DFESKLFFKPEAGRLLERELSKPGYKPQVIHIGGDTDPYQPQEKTLRVTRAVIETLQR
                     FNHPFTIITKSALIVRDLDILGPMGQAGLARAAVSVTTLDRKLARSMEPRAATPDRRI
                     DAIRQLAAAGVPVTVMFAPAIPSLNDHEMEAVLQRAAEAGATSAGYVALRLPLEINDL
                     FQQWLATDHPDRAKRVMSLVRQMRGGAAYDSEWGKRMTGEGPVAEVMNQRFLMARRKL
                     GLDEPSQRMDIGAFRVPAKAGDQLSLF"
     gene            2091..2579
                     /locus_tag="PLH31_02345"
     CDS             2091..2579
                     /locus_tag="PLH31_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR88116.1"
                     /translation="MITVVIPTHDSAHLIGPCLSSLVTAAVDGLVREVVLADGGSTDG
                     TLDIAEDCGARVVTDGADVGLRLALACASPRGDWVMLLEPDAILPEGWRLVIERQINA
                     GWDKAVSLPRPGLPWFRRPFAAQQGLLLSARRYEASGGFKAGDKGFARLIGAVGAQRL
                     VW"
     gene            complement(2581..>4012)
                     /locus_tag="PLH31_02350"
     CDS             complement(2581..>4012)
                     /locus_tag="PLH31_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQR88117.1"
                     /translation="AAFSASERGDFDAAQTAFANTRDKSLAGRLAYAKVMHPRAYTAD
                     YEDLTAWLKAHSDEAGADRVYTLAVKRKPAKAPPPRPPVLFVASEKGPPPADKARGAR
                     EAYYSGDMDKALTLAQISGERWIAGLASYRLGQHIQAQTYFDQVARDESQDDWLRAAG
                     AFWAARAASAAGRSGDARDALIEAARSPNTFYGMIAARQLSLAGVALPNDPDDPIAAL
                     ISRFSYDGPDITSLSRLLNADDRARRAAALAQIGRWAEAGQELRAGLSLAESEKARRD
                     WMSLTLAINARAPLNAGRPVARLGVEEYPLPPLEPKGGFTIHKAMVYALVRQESRFDP
                     MAVSGAGATGLMQLMPIAAARAAGDDKLLADTSPLLDPAFNLRVGQDYFTWLMDRGLQ
                     SYDMLRAVAAYNGGPGTLLKTQAQLGADCDSLLLIESLPAVETRNYVEKVVASYWTYR
                     RLMGAETPTLDAAAMGAREIDFRLDL"
     assembly_gap    4013..4022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4184..5002)
                     /locus_tag="PLH31_02355"
     CDS             complement(4184..5002)
                     /locus_tag="PLH31_02355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQR88118.1"
                     /translation="MNLALDPRAVESLLAFWADAGVDASYAEAPIDRLAEGAALLRAR
                     SQPTPAAPAPRPTLVVAGSPDITAAIAQAKAAASACNDLEALAAAIAAFDGCALKTQG
                     AKQAVVSRGPGNAPLMIIGEGPGADEDAQGAPFVGRAGQLLDRMLKAASLTDRVFITN
                     TVFWRPPGNRTPTPQEQAVCAPFLERAIALVNPRMLMLVGGASAKAMLKREEGILSLR
                     GRWFEWTASDGSNELPALPTLHPAFLLRQPAAKKKAWQDLLTLTERLDRPERPH"
BASE COUNT          740 a         1606 c         1817 g          917 t
ORIGIN      
        1 gcctgagcgg ctttgtcctg gcccacgcct atggcgcgcg gacgatgggt tggctgggct
       61 tcatgcgcgc ccgcctgatc cgtgtgtggc cgctgatcgc gctgtcgatg gtcgccggcg
      121 ccctggtgat gatcgggcac cgcgacaaca tcctgatctg cctgctgatg gggctgctgg
      181 ttttccccag aatttgggtt agcgacgagg acagcttctc accgcttttc cctttgaatc
      241 cgcccgcctg gtcgctgttt ctcgagttgc tggctagcgc cctgtggttt cccgcccggc
      301 ggctttcgac gatgtggctg gtgctggtca tcgtcgtgtc gggcggcgtg ctgatcgcca
      361 tcgcctatgg catgggcggg gtgcagaccg gctgggatcg ggccacctac tggatcggga
      421 tcatccgcac catctttccc tttgccctgg gctgggcctg ctatcggatc caggcctatg
      481 tgacggtgag ccttccggcc tggattttgg cgctggtgct gctggccgtg ctggcggttc
      541 ctcccatgga ctatacggcg acctatgatc tggtctgcgt cgcgatcgtg ttcccattga
      601 ttgtgctgct gggccatcgc gaccccacag gccgggtcgg ggcgatctgc cggttcagcg
      661 gcgagatttc ctatccgctc tatgccctgc actgggtgct ctgggagctg tcgctgcgcg
      721 ccttccgggc gatgggcggc aagggctatc ccgatgtgtt ggtcgtgact gcgattcttc
      781 tgatcatcgc tggcgcctgg ggcgtgctga aggtctatga tctgcccgtc cgtcgctggc
      841 tgcgggcgcg tctggtccgg gactgaagtc ttttttcccc gcctgctgtc ggccggcgcg
      901 acccgaacgg cgggctgagg aagcctctag aacaggctga gctggtcgcc tgctttggcc
      961 gggacccgga acgccccgat gtccatcctt tgcgacggct catcgagacc cagcttgcga
     1021 cgcgccatca ggaagcgctg gttcatcacc tcggccaccg ggccttcgcc cgtcatgcgt
     1081 ttgccccact cggaatcata ggccgcgccg ccgcgcatct ggcgcaccag cgacattacc
     1141 cgcttggcgc ggtcggggtg atcggtggcc agccactgct ggaaaagatc gttgatctca
     1201 agcggcaggc gcagggcgac atagccggcc gaggtggcgc cggcctcggc ggcgcgctgg
     1261 agcacggctt ccatctcgtg gtcattcagc gacgggatcg cgggcgcgaa catcaccgtg
     1321 acaggcaccc cggcggcggc caattggcgg atggcgtcga tgcggcggtc gggcgtcgcg
     1381 gcgcgcggct ccatgctgcg ggccagcttg cggtccagcg tggtgacgga caccgccgcc
     1441 cgcgccagac ccgcctggcc catgggcccc aggatgtcga gatcgcggac gatcagggcg
     1501 gacttggtga tgatcgtgaa ggggtggttg aacctctgca gggtttcgat caccgcccgg
     1561 gtgacccgca gggtcttttc ctgcggctga tagggatcgg tgtcgccgcc gatatggatg
     1621 acctgcggct tgtagccggg cttggacagc tcccgctcca gcagcctgcc ggcctcgggc
     1681 ttgaagaaca gcttggactc gaaatcgaga ccgggcgaga ggcccagata ggcgtgggcc
     1741 gggcgggcgt agcagtaaat gcagccgtgc tcgcagcccc gataggggtt gatcgagcgg
     1801 ctgaagctga tgtcgggact gtcgttcctg gcgatgatgg tcttggactt catcggctgg
     1861 atggtggtgg tcagcggccg ggccgcttcg tccgcctcgg tccagccatc ctcgaaagcc
     1921 tcggagcgga aagtctcgaa acggctggtg tcgtttgtgc gagctccgcg accgcgaagg
     1981 gcgaccttgt tcatgtggga cggaacagcc atgcccgacc ttagtcctga aatcagaaca
     2041 taacaagaac tacccttgac cgatgcagcc gacatcgtga agcagggagg atgatcaccg
     2101 tcgtcattcc cacccatgac agcgcccacc tgatcgggcc gtgcctttcc agcctggtga
     2161 cagcggcggt ggacggtctt gtccgtgaag tggtgctggc cgacggcggc tcgaccgatg
     2221 gtactctgga catcgccgag gactgcggcg cgcgggtcgt gaccgatgga gccgatgttg
     2281 gcctgcgtct ggccttggcc tgcgcttcgc cgcgcggcga ctgggtgatg ctgctggaac
     2341 ccgatgcgat cctgccggaa ggctggcggc ttgtgatcga aaggcagatc aatgctggct
     2401 gggacaaggc ggtcagtctg ccgcgtccgg gtctgccgtg gtttcgacgg ccgttcgcgg
     2461 cgcagcaggg cttgctgctg tcggcccggc gctatgaggc cagcggtggc ttcaaggccg
     2521 gggacaaggg ctttgcgaga ctgatcgggg ctgttggcgc ccagcggctg gtctggtgac
     2581 ctacaggtcc agccggaagt cgatctcacg cgcgcccatg gctgcggcgt ccagggtggg
     2641 ggtctcggcg cccatcaggc ggcgataggt ccagtacgaa gccacaacct tctcgacata
     2701 gttgcgggtc tcgacggccg gcaagctttc gatgagcagc aggctgtcgc agtcggcgcc
     2761 cagttgcgcc tgggtcttca gcagggttcc gggaccgccg ttataggccg ccacggcgcg
     2821 cagcatgtcg tagctctgca ggccgcgatc catcagccac gtgaaatagt cctggccgac
     2881 ccgcaggttg aaagcgggat ccagcagggg cgaggtgtcg gccaacagct tgtcatcgcc
     2941 ggcggcgcgg gcggcggcga tcggcatcag ttgcatcagg cccgtcgcgc cggcgccgga
     3001 gaccgccatg gggtcaaacc ggctttcctg gcgcaccagg gcatagacca tggccttgtg
     3061 gatggtgaag ccgcccttgg gctccagcgg gggcagggga tattcctcga cgcccaggcg
     3121 cgccaccggg cgcccggcat tgagcggggc ccgggcgttg atggccaggg tcaggctcat
     3181 ccagtcgcga cgcgccttct cggactcggc cagcgacagg ccggcgcgca gttcctgacc
     3241 ggcttcggcc cagcgtccga tctgggccag ggcggcggcc cggcgggcgc gatcatcggc
     3301 gttcagcagg cggctcagcg aggtgatatc cgggccgtca tagctgaagc ggctgatcag
     3361 ggcggctatg ggatcatcgg gatcattggg cagggccacg cccgccaggc tgagctgccg
     3421 cgcggcgatc atgccgtaga aggtgttggg cgagcgggcg gcctcgatca gggcgtctcg
     3481 cgcatccccg ctgcgtccgg ccgcgctggc ggcgcgggcg gcccagaagg cgccggcggc
     3541 gcgcagccaa tcgtcctggc tctcgtcgcg ggccacctgg tcgaaatagg tctgggcctg
     3601 aatgtgctgg cccagccggt acgacgccag ccccgcgatc caacgctcgc cgctgatctg
     3661 ggccagggtc agggccttgt ccatgtcgcc ggaataatag gcctcgcggg ctccccgcgc
     3721 cttgtcggcg ggcggcgggc ctttttcgga ggcgacgaac aggaccggcg ggcgaggggg
     3781 cggcgccttg gcgggcttgc gcttgaccgc cagcgtgtag acccggtcgg cgccggcctc
     3841 gtcgctatgg gcctttagcc aagccgtgag gtcttcatag tcggcggtat aggcgcgcgg
     3901 gtgcatgacc ttggcatagg ccaggcgacc ggccaggctt ttgtcgcggg tattggcgaa
     3961 ggcggtctgg gcagcgtcaa agtcgccccg ctcggaggcc gagaacgcgg ccnnnnnnnn
     4021 nnggacagcg gctcaaggct ggaagcgttg gcttgcaagc cggccagaca gagcatcagg
     4081 gccgacagtg cggccagagt gcgcgccaag gatcgcaaat cgtattcgcg cctgcttttg
     4141 aaaagcgacg acgcgccccc gttcccactg gtcgccagga gtcctagtgg ggacgctccg
     4201 gacgatcaag cctttcggtc agggtcagaa gatcctgcca ggcctttttc ttggccgccg
     4261 gctgtctgag caggaacgcc gggtggagcg tcggcaaggc gggcagttcg ttggaaccgt
     4321 cggaggcggt ccactcaaac cagcggccgc gcagggagag aatgccttcc tcgcgcttca
     4381 gcatggcctt ggccgaggcg ccgcccacga gcatcagcat tctgggattc accagagcga
     4441 tggcgcgttc cagaaacggc gcgcagaccg cctgctcctg cggtgtcggc gtccggttgc
     4501 ccggcggacg ccagaagacg gtgttggtga tgaagacccg gtccgtcagg ctcgcagcct
     4561 tcagcatgcg atccagcagt tggcccgcgc gacccacgaa cggcgcgccc tgggcgtctt
     4621 cgtcggcgcc agggccttcg ccgatgatca tcaagggcgc gttgcccggc ccgcgagaga
     4681 ccacggcctg cttggcgccc tgcgtcttca gcgcgcagcc gtcgaaggcg gcgatggcgg
     4741 cggccagggc ctccagatcg ttacaggcgg aagcggccgc cttggcctgg gcaatggcgg
     4801 cagtgatatc gggcgagccg gcgacgacca gggttggacg cggggcgggc gccgccggag
     4861 tgggctggct cctggcccgc aacagggccg caccctcggc caggcggtcg atgggcgcct
     4921 cggcatagga ggcgtcgacg cccgcatcag cccagaaggc gagcaggctt tcgacggcgc
     4981 gagggtcgag cgcgaggttc atggagccat ccttagcgtg atctttgccg cccctccacg
     5041 ctgcgccgca acaaaagtcg tcgctttctc cttgaccacc gatgcgtcac
//
LOCUS       DAOMEO010000063         5080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2082_length_5080_cov_2.367960, whole genome shotgun sequence.
ACCESSION   DAOMEO010000063 DAOMEO010000000
VERSION     DAOMEO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_5080_cov_2.367960"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLH31_02360"
     CDS             complement(<1..363)
                     /locus_tag="PLH31_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88119.1"
                     /translation="MEEPRSAQDADKEAVRLRATKALKAFDKLGDDPRFLRLVRLAAT
                     HFQAPHAALWLIGASRLHCKASFGGGGGVTPRQGSVADLLIALREPLICPDARSDARL
                     ASLSLKTIGFLACAPLIDP"
     gene            536..886
                     /locus_tag="PLH31_02365"
     CDS             536..886
                     /locus_tag="PLH31_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88120.1"
                     /translation="MPADVLKTAAKDADKVKSAAAAAVDHAEHSFVEAANAAKVSLAE
                     AAKRVEKSVAEGFDTLRAQSRTYTDTAGQQLDDAQRYVTERVRERPLTATLAGLGVGV
                     LIGLLIAGNRNTHR"
     gene            883..1281
                     /locus_tag="PLH31_02370"
     CDS             883..1281
                     /locus_tag="PLH31_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88121.1"
                     /translation="MIFEKTLMTLAAAAAIAAAAAVGVFAAAFALYAVVQVYLGAAGA
                     AAVVAAVAALIVAIAGLIAARKVEQRQAPSQGATPDSFGLTEKIVGIVRDRPLLSIGA
                     GLAAGVFALKNPVLTAAVFKAFMEPRQPPR"
     gene            1343..1963
                     /locus_tag="PLH31_02375"
     CDS             1343..1963
                     /locus_tag="PLH31_02375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQR88122.1"
                     /translation="MSYILPELPYAYEALEPTVSANTLHFHHDKHHAAYVTALNGLLN
                     GDDKGSLEEVIKTAAPGKVFNNAAQAWNHAFFWDAMSPEKTAPGAELAAAINEAFGDL
                     AALKTAFVTEAVGHFASGWVWLVAEGGKLKVISTHDAATTVTEAGVTPLLVCDVWEHA
                     YYLDHQNNRKGFLEAWFDTLANWTLADSQFAASKGAGEVWAYPAPQ"
     gene            complement(2018..2794)
                     /locus_tag="PLH31_02380"
     CDS             complement(2018..2794)
                     /locus_tag="PLH31_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQR88123.1"
                     /translation="MKIIQVSDIHFGGENQAATEAALARFHQEKPDLMVAAGDLTRDG
                     VVAEFDAARAWLDRAPTPLFTTPGNHDTPYVGPGELLERFIAPWRRYGPADGGVWRAP
                     GVSVFGVNTARRAQLRWNWSKGAISGSQIRRLEGQLASAPGDARIVVCHHPLMELTGE
                     PMTSEVHGGTRAANSLTMAGADLVLSGHIHTPFVSPYPFGDGKTRAVVSGTLSVRERG
                     VPPSFNIIEIDPTQIRVTAMGFERTDFVTLRSWAFDRRQA"
     gene            complement(2803..3657)
                     /locus_tag="PLH31_02385"
     CDS             complement(2803..3657)
                     /locus_tag="PLH31_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQR88124.1"
                     /translation="MPVRRVEIIANTASGSVGADAPAIAERILAEQGVAGTVHTPDGD
                     LAACLRRVIDSDPDAVFVIAGDGTARAAAEMSGPDGPPIAPLPGGTMNMLPRAFYGER
                     SWPEAMAACLTDGAPRMVSGGEVAGRLFFVAAILGSPALWADAREAARAGQIRLTALR
                     AQRAFRRAFSGRLRFTLDGRPKQKAEALTLMCPLVSTVLDEDDRALEAAAVDPSNAIE
                     IFRLGVSALSGTWRDDPSVSMGRCRVGRVWAGGRIPALLDGEPMRLGPEIQIRFRPKA
                     FRAVALPE"
     gene            3777..4118
                     /locus_tag="PLH31_02390"
     CDS             3777..4118
                     /locus_tag="PLH31_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HQR88125.1"
                     /translation="MKMTMIALLGVGLMATACTSTGNVERNAAGGAALGALAGAVIGN
                     NVGSGSASTGAAIGAALGGTAGAVKGCREDGGCGANTVNRRQYYDERAGRYYYYDAGS
                     GRYFWEDGRPR"
     gene            4205..4657
                     /locus_tag="PLH31_02395"
     CDS             4205..4657
                     /locus_tag="PLH31_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR88126.1"
                     /translation="MTIKMTVATLGAGLMLAGCASAPWSASRAAIETQPPACADFQVS
                     IYFERDEVKVTREARSVLNSARTMARGCKVEKARVVGLADAVGAADVNMDLSRARAVS
                     VTKALTRAGFGQVEFDVAAAGDIGATTTAGASAPLRRRADIFFDVSKP"
BASE COUNT          868 a         1684 c         1727 g          801 t
ORIGIN      
        1 cggatcgatc aggggcgcgc aggccagaaa gccgatcgtc ttcagcgaca gggacgccag
       61 ccgggcgtcc gagcgcgcgt ccgggcaaat caggggttcg cgcaaggcga tcagcaggtc
      121 ggcgaccgag ccttgccggg gcgtcacgcc gccgccgccg ccgaaactgg ccttgcaatg
      181 cagccggctc gcaccgatca gccagagggc cgcatgaggc gcctgaaaat gcgtggccgc
      241 caggcgcacc agccgcagga aacgcggatc atcgcccagc ttgtcaaagg ccttcaacgc
      301 cttggtggcg cgcagccgaa cggcttcctt gtcggcgtct tgcgcggaac ggggctcctc
      361 catattccga ccatagactc gcttcgcgtc aggcgcgagt ggcgataaaa attctgcagt
      421 ggtgaagctt ccgccgcgcg ttattatgga actgcacgcc gaggctccgc gttgacgggg
      481 ctaagctaga tcgccagcgc gcgccgctgg ttgcgcccgc gaaccggaga tgaccatgcc
      541 tgctgatgtc ctgaagaccg ccgcgaaaga cgctgacaag gtcaagagcg ccgccgccgc
      601 cgcggtcgat cacgccgagc acagcttcgt cgaagccgcc aacgccgcca aggtgtcgct
      661 ggccgaggcc gccaagcggg ttgaaaagtc ggtcgccgag ggctttgaca ccctgcgcgc
      721 ccagagccgc acctataccg acaccgcagg ccagcaactg gacgacgccc agcgctatgt
      781 caccgagcga gttcgcgaac gtccgctgac ggccaccctc gccggtctcg gcgttggcgt
      841 gctcatcggc ctgctgattg ccggcaaccg aaatacccac cggtgatctt cgaaaaaacc
      901 ctgatgacgc ttgcggcggc ggcggccata gcggctgcgg ccgccgttgg cgtgttcgcc
      961 gcagccttcg ccctctatgc ggttgtccag gtctatctgg gcgctgcggg cgccgccgcc
     1021 gttgttgcag cggtcgcagc tctgatcgtc gcgatcgccg gtctgatcgc tgctcgcaag
     1081 gtcgaacagc gccaggctcc cagccagggc gctacgccgg actcgtttgg tctgacggaa
     1141 aagattgtcg gcattgttcg cgatcgtccc ctgctgtcga tcggcgcggg gctcgcagcg
     1201 ggtgtcttcg cgttgaagaa tccggttctg acggcggctg ttttcaaggc cttcatggaa
     1261 ccgcggcaac cgccacgcta aatacccttc tgcctgcgtt cccgtcctcc cctccttggg
     1321 aacgccatcc catggagtga ccatgtccta catcctgcct gagctgccct acgcctatga
     1381 ggcgctggag ccgacggttt cggccaatac cctgcatttc caccatgaca agcaccacgc
     1441 cgcctatgtc acggccctca acggcctgct gaacggtgac gacaagggct cgctggaaga
     1501 ggtcatcaag accgccgccc cgggcaaggt gttcaacaac gccgcccagg cctggaacca
     1561 cgcgttcttc tgggacgcca tgagcccgga aaagaccgcg ccgggcgccg agctggccgc
     1621 cgcgatcaat gaagcctttg gcgatctggc cgccctgaag accgccttcg tcaccgaggc
     1681 cgtcggccac ttcgcctcgg gctgggtgtg gctggtcgcc gaaggcggca agctgaaggt
     1741 catctcgacc cacgacgcgg ccaccacggt gacggaagcc ggcgtgaccc cgctgctggt
     1801 ctgcgacgtc tgggagcacg cctattatct ggaccaccag aacaaccgga agggtttcct
     1861 ggaagcctgg ttcgacaccc tggccaactg gaccctggcc gacagccagt tcgcggcgtc
     1921 caagggcgcg ggcgaggtct gggcctatcc ggctccgcaa tagccacagc ggcctgaagt
     1981 gtgggacggc gcggccatgg ccgtgccgtt ttgcgtttca ggcctgccga cggtcgaagg
     2041 cccagctgcg cagcgtcacg aaatcggtgc gctcaaagcc catggccgtg acccggatct
     2101 gggtcggatc aatctcgatg atgttgaagc tgggcggcac gccgcgctcg cgcaccgaca
     2161 gcgtacccga caccaccgcc cgcgtcttgc cgtcaccgaa aggatagggc gacacgaacg
     2221 gcgtatggat atggccagac aggaccaggt ccgccccggc catcgtcagg ctgttggcgg
     2281 cccgggttcc gccatgcacc tcgctggtca tcggctcgcc ggtcagctcc atcagggggt
     2341 gatggcagac gacgatgcga gcgtcgcccg gcgcgctggc cagctggcct tccagtcggc
     2401 ggatttggct gccggagatg gcgcccttcg accagttcca gcgcaactgc gcgcgccggg
     2461 cggtgttgac cccgaatacc gacacccccg gggcgcgcca gacgccgcca tcggcggggc
     2521 catagcgccg ccagggcgcg atgaaccgct ccagcagttc gcctggcccg acatagggcg
     2581 tgtcgtgatt gccgggcgtg gtgaacaggg gtgtgggcgc gcgatcgagc caggccctgg
     2641 ccgcgtcgaa ctcggccacg acgccgtcgc gggtcaggtc gcctgccgcc accatcagat
     2701 cgggcttttc ctgatgaaac cgcgccagcg ccgcctctgt cgcggcctgg ttctcgccac
     2761 cgaaatgaat gtcggaaacc tggatgatct tgatggtcat ggtcactcgg gcaaagccac
     2821 ggcgcggaag gccttgggac ggaaacggat ttgaatctcc ggccccaacc gcatcggttc
     2881 tccatcgagc agggcgggaa tccggccgcc ggcccacacc cggccgacgc ggcagcgacc
     2941 catgctcacc gaaggatcat cacgccaggt tccgctcagc gcgctgaccc ccaggcggaa
     3001 gatctcgatg gcgttggacg gatcgacggc cgccgcctcc agggcccggt cgtcttcgtc
     3061 caggaccgtg gagaccaggg ggcacatcag ggtcagggct tcagccttct gcttgggtcg
     3121 accatcaagg gtaaagcgca ggcggcctga aaaggcgcgg cgaaaggccc gctgggcgcg
     3181 cagggcggtc agccggatct gtccggcccg cgcggcctcg cgggcgtccg cccaaagggc
     3241 gggactgccc aggatggcgg cgacgaagaa caggcgtcca gccacctcgc cgcccgacac
     3301 catgcgcggc gcgccgtccg tcaggcaggc cgccatggcc tcgggccacg agcgctcgcc
     3361 gtagaaggcg cgaggcagca tgttcatcgt cccgccgggc aggggcgcga tgggcgggcc
     3421 gtcgggacct gacatctccg cagcggcccg ggccgtgccg tcgccggcga tgacaaagac
     3481 ggcgtcagga tcggaatcga tgacgcggcg caggcaggcg gccagatcgc catcgggcgt
     3541 gtgcaccgtt ccggctacgc cctgttcagc gagaatgcgc tcggcgatgg ccggcgcatc
     3601 ggcgccaact gatcccgaag cggtgttggc gataatttcg acgcgacgca cgggcatcct
     3661 cgctgcatgg ggagtcggtt aacggccaag cttttcggcg gttccgagcc tcacaacgga
     3721 accttgcata cggcgggtcg ttagtcagac cgggatagct cgatgggaaa cccactatga
     3781 aaatgaccat gatcgccctg ctgggtgtcg gcttgatggc cacggcctgc acatcgaccg
     3841 gcaatgtaga acgtaatgcc gccggcggcg cagcgcttgg cgcgctcgca ggcgctgtga
     3901 tcggcaataa tgtgggcagc ggcagcgcca gcacgggcgc agccatcggc gcggccctcg
     3961 gcggcaccgc tggcgccgtc aagggctgcc gtgaagacgg cggttgcggc gcgaacacgg
     4021 tcaatcgccg ccagtactac gatgaacgcg cgggccgcta ttactattac gacgctggca
     4081 gcggccgata cttctgggaa gacggtcgtc cccgttaggg agcgatcgcg ccgatgaaaa
     4141 acccaagggg cggccggcag attgctggtc gccccttgtc atgtccatca cacggggagg
     4201 cactatgacc atcaagatga ctgtggcgac gcttggcgcc gggctgatgc tcgcgggctg
     4261 cgcttccgcg ccctggagcg cgagccgcgc cgcgatagaa acccagccgc cggcctgcgc
     4321 cgatttccag gtttccatct attttgagcg ggacgaggtg aaggtgacgc gtgaggcgcg
     4381 atcggtgctg aacagcgccc gcaccatggc gcgcggctgc aaggttgaga aggctcgtgt
     4441 tgtcggcctg gctgacgccg tcggcgcggc ggacgtcaat atggatctgt ccagggcgcg
     4501 ggcggtctcg gtcaccaagg ccctgacccg ggcgggcttt ggccaggtag agttcgatgt
     4561 cgccgcggcc ggggatatcg gcgcaacaac cacagccggg gcttccgccc cgctgcgccg
     4621 acgggccgat atcttctttg acgtcagcaa gccctgaggc ctgaaacgcc gagacgcccg
     4681 ccctggtcag ctggggcggg tttcctgccg ggcgcatgac gtgcgggctt gaagccgtga
     4741 cggaaaaggg gggaggggtg cggaggcggc aggctggctt gcgccggcct caagtcgggg
     4801 gggcgtcgcc gcctccgcac tgaggaaaac gcgatctcca aaagctggtt cccggcggcg
     4861 gcgatttttt tttgcggccg gtggaaatcg ccgtccgcct gggcgccatg gaacagtcgg
     4921 gggtcgcaaa cgttgttctg gcgcggccaa gcagggccgc attccaacca agagagcacg
     4981 tcgtgatccg gagctaccag gctgaacatg gcggaacggt tttctacgcg gcgcccagcc
     5041 ggcgtcagaa gacgaggcgc acgcgcctca tggcgctgat
//
LOCUS       DAOMEO010000064         5054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2088_length_5054_cov_2.531706, whole genome shotgun sequence.
ACCESSION   DAOMEO010000064 DAOMEO010000000
VERSION     DAOMEO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_5054_cov_2.531706"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="PLH31_02400"
     CDS             <1..2187
                     /locus_tag="PLH31_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88127.1"
                     /translation="ISALVVPISSIALQVSFLVVLGVGGFRVATGAIEIGDLVAFILF
                     LFLLNVRTTVISLMAIPVSLLATALVFHYFGLSINTMTLGGLAVALGVVIDDAIVGVE
                     NIVRRLREDRSGTDRVGIIEAASVEVRAPVVYATYVLALTIAPILFLTGLQGAFFGPL
                     AFAFLLATLASLAVAITVTPALSLLLLRRIRLHDEPGYLRKLKSLHIRVLEPICARPG
                     VVVAGAALVGVLALGAFFVFGSELLPAFRERHYVLQVNGPAGASMGWMRDLGVRITKD
                     LVAIPEVATVEQQIGRAEAGEDTFPPHRSEFHVELRPVGGAGEDRVLARIREVLAQYP
                     GVQSEALTFLGDRISESLSGETAKVAVNVYGPDLDVLDRVAADVARALQSVPGAADVQ
                     VKAQPGAPTLKIALDSDRMALRGVAPTDAYDAIETAFQGRTVAQVTGADRITEVAIAL
                     PPEMQRDPEGIGALLVRTADGATTSLSDIATIRLGEGRTVINHDGGQRRQVVTANPTG
                     ADVSGFVRDARAKIASTVSLPPGVFLEFTGVAQGQAAAASQILVNVAAASIGIVALLV
                     LAFGGGRPAGLILAGTPFALAGGVAAVALTGGVLSLGALVGFVTLFGIAARNAILLIS
                     HVDHLVSYEGQPWGLATVLRATQERVTPILMTALVTGLGLAPLALEAGQSGREVQGPM
                     AAVILGGLITSTLMSLLLLPALVLAYRYPPGQPHERSRDAPAADRG"
     gene            2162..2833
                     /locus_tag="PLH31_02405"
     CDS             2162..2833
                     /locus_tag="PLH31_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR88128.1"
                     /translation="MRLLLIEDDAETAAFVVEGLSARGHACETVMDGQAGLVTAMAGS
                     FDVMIIDRMLPSLDGLSVAKALRDAGRETPILILTALGSVEDRVAGLEGGADDYLVKP
                     FSMSELAARVDALGRRGASASPTRLAIADLTLDRLTRQVRRGGELVDLQHREFQLLEA
                     LMLQTPSVVTRTMLLEGVWKIRFDPGTSLVESHVSRLRAKIDRAGSPMLIHTVRGEGY
                     AARTP"
     gene            2814..4262
                     /locus_tag="PLH31_02410"
     CDS             2814..4262
                     /locus_tag="PLH31_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR88129.1"
                     /translation="MPLARLSPLWRSTSFRISLIHAALLAAAIVGAGTGGWVTTRGAA
                     EREAHDRIALEVHAVTLEIQAEGLKAAGDAVMARAERPGALEYRLVDSTGRHVAGDLT
                     DLGPKEGWRSLDFDGTAPGLEGKDRLIILTQRLPDGARLTVGEDLGRAEALRNTLFRT
                     LLLWGGLALALGLAAGLYLTHRALERMNALVRTVRTVADGDLAARASDARPEGDDIGQ
                     LAAGVNQMLDRIGVLMAAVRRVSAEVAHDLRTPLSHVRQRLDAAAAAATPELRNAALQ
                     SANDGIDRALRMFDAMLRLAEIDAGAARARFAAVDLMDLTDRVADAYRAELEASGRTL
                     IVQIAECRVVEGDVDLLTQALANLLENALKHSRAGAAVRVRLVEEPDGIKLSVEDDGP
                     GIPPEMFGVAVRPFSRLDPARAAPGTGLGMAITASVARLHGGRLLLEDARPGLKVTML
                     MVPAQGPMRSQNDASCDRGRPDLAAVRDADGN"
     gene            4231..>5054
                     /locus_tag="PLH31_02415"
     CDS             4231..>5054
                     /locus_tag="PLH31_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88130.1"
                     /translation="MQPFVTPMGTDKTKALWRSGISAVPRGVWALGLASMFMDISSEM
                     IHSLLPVFLVGTLGASVALVGLIEGAAEATASITKIFSGWLSDRLGKRKLLAVLGYGL
                     GALSKPIFPLAVTPFEVFGARFADRIGKGVRGAPRDALVADLSPPSIRGAAFGVRQSL
                     DTIGAFTGPLVAMALMLVLAGNIRAVFAWAAVPAIVAVLLLVFGVQEPRAPAGAHGAA
                     RPPIRWAEVRGIGPAFWGVVALGVVFTMARFSEAFLVLRAQDAGLPVALLPVVLVV"
BASE COUNT          700 a         1685 c         1717 g          952 t
ORIGIN      
        1 atctcggccc tcgtcgtccc catctcgtcg atcgcgctgc aggtgtcgtt cctcgtcgtc
       61 ctcggcgtcg gcggcttccg cgtcgcgacg ggcgcgatcg agatcggcga cctcgtcgcg
      121 ttcatcctct tcctgttcct gctcaacgtg cgcaccaccg tgatttcgct gatggcgatt
      181 ccggtgtcgc tgctggccac ggcgctggtc ttccactact tcggcctgtc gatcaacacc
      241 atgaccctgg gcggcctggc ggtcgccctc ggcgtggtca tcgacgacgc catcgtcggg
      301 gtggagaata tcgtgcgccg ccttcgcgag gatcggtccg gaaccgacag ggtggggatc
      361 atcgaggcgg cgtcggtgga ggtgcgcgcg ccggtcgtct atgcgaccta cgtgctggcc
      421 ttgaccatcg cgcccatcct cttcctgacc ggcctgcagg gcgcattctt cggtccactc
      481 gcctttgcgt tcctgctggc gacactggcc tccctcgcgg tcgccatcac cgtgacgccc
      541 gcgctgtcgc tgctgctcct gcgtcggatc cggctacacg acgagccggg ctatcttcgg
      601 aagctgaaga gccttcatat tcgggtctta gagccgatat gcgcgcggcc cggcgtggtc
      661 gtggcgggcg ccgcgctggt cggtgtgttg gcgctagggg ccttcttcgt ctttggctcg
      721 gagctgctgc cggcgttccg cgagcgtcac tatgtgctgc aggtcaatgg gcccgccggc
      781 gcctcgatgg ggtggatgcg cgatcttggc gtccgcatca ccaaggatct ggtggcgata
      841 cccgaggtcg cgacggtcga gcagcagatc gggcgcgccg aggccggaga ggacaccttt
      901 cccccgcatc gcagcgagtt ccatgtcgag ttgcgccccg tgggcggggc cggcgaggac
      961 cgggttctgg cgcgcatccg agaggttctg gcgcaatatc ccggcgttca gagcgaagct
     1021 ctgaccttcc tcggagaccg catcagcgaa tccttgtcgg gggagacggc gaaggttgcg
     1081 gtcaatgtct atggacccga cctcgacgtt cttgaccgtg tcgcggccga tgtggctcgc
     1141 gccctgcagt cggtccccgg cgcggccgac gtgcaggtga aggcccagcc cggcgctccg
     1201 accctgaaga tcgccctgga ctcagaccgc atggcgcttc gcggcgtcgc ccctaccgac
     1261 gcctatgacg ccatcgaaac ggccttccag ggccgcaccg tggcgcaggt cacgggtgcc
     1321 gatcgcatca ccgaggtggc catcgccctc ccaccggaga tgcagcgtga tccggaaggc
     1381 atcggagcct tgctcgtgcg caccgcagat ggtgcgacga cctccttgtc agacatcgcc
     1441 accattcgct tgggcgaggg gcgcaccgtc atcaatcacg acggcggtca acgtcggcag
     1501 gtggtcaccg ccaatcccac gggggcggac gtctccgggt tcgtgcgcga cgcccgcgcc
     1561 aagatcgcaa gcaccgtttc gctcccgccg ggtgtgttcc ttgaatttac cggcgtagcc
     1621 cagggtcagg ccgctgcggc tagccagatc ctggtcaatg tcgcggccgc ttcgatcggc
     1681 atcgtcgccc tcctcgtgct ggccttcggc ggcggtcgtc ccgccggact gatcctggct
     1741 gggacgccgt ttgcgctggc cggcggcgtc gcggcggtgg ccttgaccgg cggggtgctg
     1801 tcactgggtg ccctggtcgg cttcgtcacg ctattcggaa tcgccgcccg caacgccatt
     1861 ctcctgatct cccacgtcga tcatctagtg agctatgagg gccaaccctg gggcttggcg
     1921 acggtactgc gagcgaccca ggaacgcgtg acgccaatcc tgatgaccgc cctggtcacc
     1981 ggacttggcc tggcgccgct cgcgctcgag gcgggccaat ccggacgcga ggtgcagggt
     2041 ccgatggccg cggtcatcct cggcggattg atcacctcga ccctgatgag cctgctgctg
     2101 ctccctgcgc tcgttctcgc ctatcgttat ccgccgggac aaccccacga gagatcacgc
     2161 gatgcgcctg ctgctgatcg aggatgatgc tgagactgcg gccttcgtcg tcgagggcct
     2221 cagcgcgcgt ggtcatgcgt gcgagaccgt gatggatggc caggccgggc tggtcacggc
     2281 gatggctggc agcttcgacg tgatgatcat cgaccggatg cttcccagcc tggacgggct
     2341 gtcggtcgcc aaggccctgc gcgatgccgg gcgtgaaacg cccatcttga tcctgacggc
     2401 gctcggctcg gtcgaggatc gggtcgccgg cctggagggg ggcgctgacg actatctggt
     2461 caagccgttc tcgatgtcag aactggcggc ccgcgttgac gccctcggcc gcagaggcgc
     2521 atcggcttcg cccacccggc tggccatcgc cgatttgacg ctcgatcgtt taacgcgcca
     2581 ggtccgacgg ggcggcgaac tggtcgatct gcagcatcgc gagtttcagc tgctcgaagc
     2641 cttgatgctt cagacgccca gtgtcgtcac ccgcaccatg ctgctggaag gcgtttggaa
     2701 aatacgcttt gatccgggca ccagcctggt ggagagccac gttagccgcc ttcgcgccaa
     2761 gattgatcgc gcggggagtc caatgctgat tcacaccgtg cgaggagaag gctatgccgc
     2821 tcgcacgcct tagcccgctc tggcgctcga ccagctttcg gatctccctc atccacgccg
     2881 cgctactggc ggctgcgatc gtgggcgcag gaaccggcgg ctgggtgacg acccgcggcg
     2941 cggccgagcg cgaagcccat gatcgcatcg cccttgaggt ccacgccgtc acgcttgaga
     3001 tccaggccga gggtctgaag gccgccggcg acgcggtgat ggcgcgcgcg gaacgacccg
     3061 gcgccctgga ataccgtctg gtcgattcca cgggccgcca tgtcgcgggc gacttgaccg
     3121 acctgggccc caaggagggg tggcgaagcc tcgactttga tggaaccgcg cccggactgg
     3181 aaggcaagga ccgcctcatt atcctgaccc agcgcctgcc ggatggtgcg cggctcacgg
     3241 tcggagagga ccttgggaga gccgaagcgc tgcgcaacac gctctttcgg accctgttgc
     3301 tatggggcgg tttggccttg gccctagggt tggcggccgg cctttacctg acacaccggg
     3361 cgctcgaacg aatgaacgcc ttggttcgca ctgtgcgcac cgtcgccgac ggcgacctcg
     3421 cggcccgcgc ttccgacgcc aggccggaag gcgacgacat cggccagctg gctgcgggcg
     3481 tcaatcagat gctggatcgc atcggtgttt tgatggccgc cgtgcgccgg gtttccgccg
     3541 aggttgccca tgatcttcgg acccccttgt cgcatgtccg ccagcggctc gacgcggccg
     3601 ccgccgctgc gacgccagaa ctccgaaacg ccgcccttca gtcggcgaat gacggcatcg
     3661 acagggccct gcgaatgttc gacgcgatgc ttcgcctcgc ggagatcgac gccggcgcgg
     3721 cgcgggcgcg tttcgcggcc gtcgacctga tggatctgac cgatcgggtc gcggacgcct
     3781 accgcgcgga gttagaggca tcggggcgga ccttaatcgt ccagatcgcg gaatgcaggg
     3841 tggtagaggg cgacgtcgac cttctgaccc aggcgctcgc caatctgctg gagaacgccc
     3901 tgaaacattc ccgcgcgggc gccgcagttc gggtgcgcct ggtcgaggag ccggacggaa
     3961 tcaagctcag cgtcgaggac gatggacccg gcattccgcc tgagatgttc ggggtcgcgg
     4021 tccggccgtt ctcccggctg gaccctgctc gcgcggcgcc gggcacgggt cttggcatgg
     4081 cgatcaccgc tagcgtcgcc cgcctgcacg gcggacggct gcttctcgaa gacgcaaggc
     4141 ccggcttgaa agtgaccatg ctcatggtcc cagctcaggg gcctatgcgt tcccagaatg
     4201 acgcctcttg cgatcgcggc cgtccggacc ttgcagccgt tcgtgacgcc gatgggaact
     4261 gacaagacga aggcattatg gcgctccggc atctcggctg tcccacgagg ggtatgggcg
     4321 ctcggcctgg cgtcgatgtt catggatatc tcttcggaga tgatccacag cctgctgccg
     4381 gtgttcctgg tcggaaccct gggggccagc gtcgcgctgg tcggactcat tgaaggggcc
     4441 gctgaggcca cggcttcgat caccaaaatc ttctccggct ggctgagcga caggctcggc
     4501 aagcgcaagc ttctggccgt tctaggctac ggcctcggag ctctgagcaa gcctatcttt
     4561 cctttggcag tgaccccgtt cgaggtgttc ggcgcccgtt tcgccgaccg gatcggcaag
     4621 ggcgtgcgcg gcgcgccgcg agacgcgctg gtcgccgatc tgtcgccccc ctctattcgc
     4681 ggtgcggcct ttggcgttcg ccaatctctc gacaccatcg gcgcgttcac gggcccgctg
     4741 gtggcgatgg cgctcatgct ggtgctcgcc gggaatatcc gcgcggtctt tgcgtgggct
     4801 gccgtcccgg ccatagtggc ggtcctgctg ctggtgtttg gcgttcagga gccgagggcc
     4861 cccgcgggcg cccacggcgc ggcgcggcca cccattcgat gggccgaggt gaggggcatt
     4921 gggccggcgt tctggggtgt cgtcgccctg ggcgttgtct tcaccatggc tcgcttcagc
     4981 gaagccttcc tggtccttcg ggctcaggat gccggtctgc cggtcgcgct gctcccggtt
     5041 gtcctggtgg tgat
//
LOCUS       DAOMEO010000065         5025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2101_length_5025_cov_2.580885, whole genome shotgun sequence.
ACCESSION   DAOMEO010000065 DAOMEO010000000
VERSION     DAOMEO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2101_length_5025_cov_2.580885"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>294)
                     /locus_tag="PLH31_02420"
     CDS             complement(<1..>294)
                     /locus_tag="PLH31_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88131.1"
                     /translation="EAPGYSPLEAEQRITFPVETAVAGVPGLQYTRSVSRYGLSQVTV
                     IFDDGTDIFFARQLVNERLQNALSQLPPGIRPELGPIATGLGEIFICLAQQRQL"
     assembly_gap    295..304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<305..458)
                     /locus_tag="PLH31_02425"
     CDS             complement(<305..458)
                     /locus_tag="PLH31_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88132.1"
                     /translation="MLERIIAQSIRFRWLVMALVLVSAAVGVWSFQRLPVDATPDITN
                     VQIQINT"
     gene            complement(473..1729)
                     /locus_tag="PLH31_02430"
     CDS             complement(473..1729)
                     /locus_tag="PLH31_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR88133.1"
                     /translation="MTPVQSRRVAPFALILSACLIVGCGDGSKTPKTEAGAEAGATEF
                     ERGPHNGRLLRDGDFSLEITLYEEGPEPLFRLYPYLKNKPLDPRQVQVAIALTRLGPK
                     VDRFTFTPEADYLASPAVVFEPHSFDVSITATRDGARSAWTYQSYEGRTVIKADAARA
                     GGVKTERAGAALLGETLPISGRVEITPEGQSEVFARYPGRVVALNASLGQRVKRGQVL
                     ARVESSESLQTYSITAPISGVILTKTVNVGTMAGTDPMLTIGDPTRLHAEFFLYPKDA
                     ERVRVGQRVEVVSQAGAHRATGVIEAILPTADPISQTLVAHVELPFQGGVWRPGLGVQ
                     GVVQVGQGDVPLAVRTKALQPFREFTVVYAKVGETYEVRMLTLGRRTDEWTEVLGGLE
                     PGTEYVSDGAFLIRADIEKSGASHDH"
     gene            complement(1729..3021)
                     /locus_tag="PLH31_02435"
     CDS             complement(1729..3021)
                     /locus_tag="PLH31_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR88134.1"
                     /translation="MSFKFRCRANARLSMAVTAMLALSLVNLTMAKAQTAPPALSLTE
                     ALTKAATSDPTLAGSEARVAAAQANQRQAGVGPNPTLGVELENFAGTGAYSLLDRTEA
                     TVSYQQTLERGGKREARTGRARAEVEVARLRRAVRTLDLLRDVQVSYAEAAAAEADLL
                     IAQARFIAAHRSQTDIDRRVKAARDPLFAGSRADMMTAQAQIARDQAQDRARNARVEL
                     ARFWGGGQEFRLALEPFFEAKGPVIPATLTVADLALLEAERDVAVANIRLEQSRAVVD
                     PTVRAGVRYLGDGNNLAFIVGGTIPLRRFDTNRSGIERAQAERNAAEADIEAARNARE
                     REIAQLVARILSGTTEVERIRTEVIPPAMRAVEQVRDGFNRGGFSYLDVTEAERALAD
                     ARARRVSVLRQYHLDQAALDRLTGRQASYIQTQTAETR"
     gene            3247..3417
                     /locus_tag="PLH31_02440"
     CDS             3247..3417
                     /locus_tag="PLH31_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88135.1"
                     /translation="MRGSVRRDIIDRADLLVMILLMVEVVIVSQMNFAAKKGRRVVSH
                     LTMLSLIHRSSD"
     gene            complement(3571..4917)
                     /locus_tag="PLH31_02445"
     CDS             complement(3571..4917)
                     /locus_tag="PLH31_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88136.1"
                     /translation="MLSVLADRTYRHLFLAQVIALVGTGLATVALGLLSYELAGANAG
                     VVLGGALAIKMIAYIGVGPIVNAFVDRLPRRAFLVSMDLVRAAVAVMLPFVTEIWQIY
                     LLIFLLQSASAAFTPTFQAVIPDILPDEKRYTRALSLSRLAYDMESLVSPILAASLLT
                     VISFHWLFGGTVIGFMASAALVVSVVVPQPKAVKREGGIYARTTRGIRIYLKTPRLRG
                     LLALNLAAAAAGAMVIVNSVVYVRTLIGRSSDDMALALAAFGGGSMATALLLPRVLER
                     VGDSTTMVAASWLLTIALVGMAILTVAPGVDRWVALLILWAILGVGYSAVLTPSGRLL
                     RRSAAPDDLSAVFAAQFALSHGCWLLTYTVAGSLGAAFGMAPTLMALAALSLAGVIVA
                     LRAWPAVDPEQLAHLHPDLPPQHSHLREGEPSSAPAGHQHPFVIDDLHPNWPRGVM"
BASE COUNT          970 a         1527 c         1639 g          879 t
ORIGIN      
        1 gagttggcgt tgttgcgcga gacagatgaa gatttcgccc agaccggtcg cgattggacc
       61 cagctctggc ctgatccccg gcgggagttg gctaagcgca ttctgcaaac gctcattcac
      121 caattggcga gcgaagaaga tgtcggtgcc gtcgtcaaat atgacggtga cttgggacag
      181 gccgtagcgc gagaccgacc gggtgtactg gagacccggc accccggcga cggcggtttc
      241 gaccgggaag gtaatgcgct gttcagcctc cagaggtgaa tagcccggag cctcnnnnnn
      301 nnnncggtgt tgatctggat ctggacgttg gtgatgtcag gcgtagcgtc gacgggcagg
      361 cgctggaagc tccagactcc gacggccgct gagaccagga cgagggccat gacaagccat
      421 cggaagcgga tcgattgggc gataatgcgt tcaagcatgg tctgcgcgct ccctagtggt
      481 catggctcgc gccggacttt tcgatgtccg cgcgaatgag gaaggcgccg tcgctgacgt
      541 actcggtacc aggctccagc ccgccaagca cctcggtcca ctcgtcggtg cggcggccca
      601 atgtgagcat ccgcacctcg taggtctcac cgaccttggc gtagacaacc gtgaactccc
      661 gaaagggctg tagggccttg gtgcgcaccg cgagcggaac atcgccctgc ccgacctgga
      721 ccacgccctg gacccctagg cctgggcgcc agacgccgcc ctggaacggt aactccacgt
      781 gagcaacaag ggtctgactg attggatcgg ccgttggcag tatggcctcg atcacccctg
      841 tggctcggtg agcgccggct tggctgacga cctcgacccg ttgaccgacc cggacgcgct
      901 cggcgtcctt cgggtagagg aagaactcgg cgtgcagtcg cgtcggatca ccaatggtca
      961 gcattggatc cgttccggcc atcgtcccga cattgaccgt cttggttaga attacccctg
     1021 agatcggggc ggtgatcgag taagtctgca ggctctcgct cgactccact cgcgccagaa
     1081 cctggccccg cttcacgcgc tgccccaagg aggcgttgag agcgacgacc cgtcctgggt
     1141 agcgagcaaa gacttcgctt tggccttcgg gggtgatctc aacacggccc gatatgggga
     1201 gtgtctcgcc gagcaaggca gcccctgctc gctcggtctt cacaccgcca gctctggcgg
     1261 cgtcggcctt gatcaccgtg cggccttcat aggattggta ggtccacgcc gagcgcgccc
     1321 cgtcgcgcgt ggctgtgatg ctaacgtcaa aggagtgagg ttcgaagacc actgccggac
     1381 ttgccaggta gtctgcctca ggcgtgaagg tgaagcgatc gaccttgggg cccagacggg
     1441 taagcgcgat cgcaacctgg acctggcgcg gatccagcgg cttgttcttc aaatagggat
     1501 agaggcggaa cagcggctct gggccctctt cataaagggt tatctcaagg ctgaaatctc
     1561 cgtcgcgcag cagacgaccg ttatggggcc cgcgttcgaa ctcagttgca ccagcctcgg
     1621 cgccggcttc ggttttggga gtcttggagc catcgccaca gccgacaatg aggcatgcag
     1681 agaggatgag ggcgaaaggc gcaacgcgcc tgctttggac gggcgtcatt agcgggtctc
     1741 cgcagtctgg gtctgaatat agctagcctg acggccggta agccgatcga gagcggcttg
     1801 atcaaggtgg tattggcgca aaacgctgac gcgacgagcc cgtgcgtcgg cgagcgcccg
     1861 ctcggcctcg gtaacgtcca gatagctgaa gccgccgcga ttgaacccgt cgcgcacctg
     1921 ctcaactgcc ctcatcgctg ggggaatgac ttcagtgcgg atccgctcga cctcggtcgt
     1981 gcccgacagg atccgggcga ccagttgagc gatctcgcgt tctctggcgt ttcgcgcagc
     2041 ctctatgtcg gcttcagcgg cattccgctc tgcctgagcg cgctcaattc ccgatcgatt
     2101 ggtatcgaag cgccgcagcg ggatagtgcc gccgacgatg aaggcgagat tatttccatc
     2161 gccgaggtag cggaccccgg cgcggacggt tgggtcaaca acggcccggg attgttcgag
     2221 gcggatattg gcgacggcta cgtcccgttc agcctcaagg agcgcgaggt cggcgactgt
     2281 cagagtggcc gggatcacag gtcccttggc ctcaaagaac ggctcgaggg ctaagcggaa
     2341 ctcctgcccg ccaccccaga accgggcgag ctcaacacga gcgttgcggg cgcgatcctg
     2401 agcttggtca cgcgctatct gcgcttgagc cgtcatcatg tcggcccggg agccagcgaa
     2461 gagcgggtcg cgcgcagcct tcacccgccg atcaatgtcg gtttgagacc ggtgagcggc
     2521 gatgaagcga gcttgggcaa taagcagatc ggcctcggct gccgccgcct cagcgtagga
     2581 aacttggaca tcccgcagca gatcgagtgt gcgcaccgcg cgccgtagcc gggcgacctc
     2641 gacttcagcc cgcgcccgcc ccgtgcgggc ctcacgcttg ccgccccgtt caagggtctg
     2701 ctggtaactg accgtcgctt ccgtccggtc aaggagggaa taggcgccgg tcccggcgaa
     2761 attctccagc tccaccccca gcgttgggtt aggccccacc ccggcttgac gttgattggc
     2821 ctgcgccgcc gcaacacggg cctccgaccc ggcgagggtc ggatcggagg tcgctgcttt
     2881 ggtcagcgcc tcggtcaggc tgagcgcggg tggcgcggtc tgcgcctttg ccatcgttaa
     2941 gttgacgagg ctgagtgcga gcatggccgt aacggccatg ctcagccgcg cgttggcgcg
     3001 acaccggaat ttgaatgaca tgtgtctctc gaagcatggg tgatcaggcg cgcacggcct
     3061 gattgccgcg ctacgtcagg cgaagacttc gagaggggct cttggtgggc gttctaggcc
     3121 caggatcatt cctgaagatg gtggcgcatc gctgggcgaa cgcatcggca aagcctgcgc
     3181 caaaattacc gaggtggcga catcgcaagg aaggggcgca atctgaggac cgtcaccgtg
     3241 atggtggtgc gagggtccgt ccggagagac atcatcgacc gcgctgacct gctcgtcatg
     3301 atcctcctga tggtcgaggt ggtcatcgtg tcccagatga acttcgccgc caagaaagga
     3361 cgcagggtgg tcagccatct gactatgctg agcttgatcc accgtagcag tgattgactg
     3421 ggaggctagc acagccacca acgccacgca gagcgtggcg aggaacgacc tcagctttct
     3481 ggctcgcaga gcaatcaccc gttagcactc ccggtatcgg ctattaggtc gtgtggcttt
     3541 tgagcgcaag ggtgggaggc tggcatgcga ctacatgacc ccccgcggcc agttggggtg
     3601 aagatcgtcg atcacaaagg ggtgctggtg ccccgcaggt gcgctagacg gctcgccctc
     3661 gcgtaggtgc gagtgctgag gcggcaggtc tgggtgtagg tgcgcgagtt gctctgggtc
     3721 gaccgccggc caggccctga gtgcgacaat gacgccggct aggctcaaag cggcgagcgc
     3781 catcagcgtc ggggccatgc caaacgctgc cccaagagag cctgctacgg tgtaggttag
     3841 cagccagcag ccgtgggaca gtgcgaactg cgcggcgaag acggcgctga ggtcatcggg
     3901 cgccgcggat cggcgcaaaa gacgtcctga cggggtcaga acagcagaat agcctactcc
     3961 caagatcgcc cataggatca ggagcgcgac ccagcgatcg acgccgggcg caactgtaag
     4021 gatggccatg ccgaccaggg cgatggtcag aagccacgag gcggcgacca tagttgtgct
     4081 atcgcccacg cgctctagta cacgcgggag cagcaaggct gtggccatgg agccgcctcc
     4141 gaaggcggct aacgccagag ccatgtcgtc actcgatctg ccgatcaatg ttcgcacata
     4201 gacgacggag ttaacgatca ccatggcgcc ggcagcggca gctgccaaat tgagcgccag
     4261 caggcctcgc aacctcgggg tcttgaggta aatgcgaata cctcgagtgg tgcgtgcgta
     4321 gatcccgcct tcgcgtttca ccgccttcgg ctgcgggaca acgacggaaa ccaccaaggc
     4381 ggccgaggcc atgaagccga tgacggtccc gccgaagagc caatggaaac tgatgaccgt
     4441 cagcagcgag gcggcgagta tcgggctgac aaggctttcc atgtcgtagg ccaagcgcga
     4501 gagcgataac gcccgcgtgt agcgcttctc gtcgggaagg atatccggga tgacagcctg
     4561 aaacgtcggc gtgaacgccg ctgaggccga ctgcaagagg aagatgagca ggtagatttg
     4621 ccagatctca gtaacgaagg gcagcatgac agccacggct gcacgcacca aatccatcga
     4681 tacaaggaaa gcgcgccttg gaagacgatc cacaaaagcg ttcacgatag ggccaacgcc
     4741 gatataggcg atcatcttga tcgctaaggc gccgccaagc acaacgccag cgttcgctcc
     4801 ggccagttcg taagacagca gacccagcgc taccgtcgct aggcccgtgc caaccagcgc
     4861 aatcacctgc gccaggaaca ggtgtctgta ggttctgtcc gcaagaacgc tcaacattgc
     4921 caatccctcc ggtggctcat cacagcgagc cccgttgagg cgccgccata tcactggggg
     4981 ttgggctctt cccctgatcc ttcaaccgct cgacgcggcg cttca
//
LOCUS       DAOMEO010000066         5002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2109_length_5002_cov_2.833030, whole genome shotgun sequence.
ACCESSION   DAOMEO010000066 DAOMEO010000000
VERSION     DAOMEO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_5002_cov_2.833030"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="otsB"
                     /locus_tag="PLH31_02450"
     CDS             <1..553
                     /gene="otsB"
                     /locus_tag="PLH31_02450"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HQR88137.1"
                     /translation="DLDRILERGVTPVAAVHGLVRRDAAGQISRTPPHPGVADARVLL
                     RELARCDKDLQFEDKGVSVTLHYRNAPAAGEAVIEAAERIARTTGLVLQLGDMVVELR
                     TPGQDKGQSVAAFLREHPFTGSTPIFVGDDLTDEDGFAAALQLGGYGVLVGEMRATSA
                     AYRLKDTAAVSAWLEGVATLMPA"
     gene            550..2058
                     /locus_tag="PLH31_02455"
     CDS             550..2058
                     /locus_tag="PLH31_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HQR88138.1"
                     /translation="MSRLIVVSNRVNPPPSPEKGDGDGSVGGLAMALAAALREYSGIW
                     FGWSGKTTPQFTGQLAMQRVDGVTTATVDLEDGDYQEYYNGYANKTLWPLFHYRVDLT
                     AYDRSFGEGYDRVNKRFAETLRPLIEPDDIIWVHDYHLIPMARELRRMGVTNRIGFFL
                     HIPWPAHQLVVTLPRHRPLVEAMFDYDLIGFQTEESRHAFEGYVTSEVDGSVAPNGEL
                     TAFGKTTRAATFPIGIDAEDFARIIESPRARKVYDRMLANSVFRKMVVGVDRLDYSKG
                     LEERLIGFERFLHDNPDMRREVMLLQIAPLSRDEVEAYQDLRSRLDGLIGRINGAYAE
                     MDFTPIRYVNKSYRRDELAGIYRAAKVGLVTPLRDGMNLVAKEYVASQDPEDPGVLIL
                     SRFAGAARQLSDALIVNPNSPEEISDALSRALAMGLPERKRRWETLFDNVQRKDVTAW
                     RDDFVGALTASTQEIQPVTPPMSLQEASTSAYVEARAAKILPATATENRRQS"
     gene            complement(2055..2483)
                     /locus_tag="PLH31_02460"
     CDS             complement(2055..2483)
                     /locus_tag="PLH31_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88139.1"
                     /translation="MSGFWRNWLAVWCGAVGVFGAVLVGGGLEVTSGPTRLIFDILNG
                     PMELDLNAQIRFSLAVMGAVTLGWAITAAATIRAAILLGERGTPIWRLLLGGVMVWFV
                     IDTAMSILTGYGNNAIPNLVLLTTFLVPIIRSGVLQQARG"
     gene            complement(2601..2969)
                     /locus_tag="PLH31_02465"
     CDS             complement(2601..2969)
                     /locus_tag="PLH31_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQR88140.1"
                     /translation="MHITSAESHVMEALWRREPLTADELVAEVGASQTWGEATVKTLI
                     NRLLKKKAIKSERADGKHGYRPLIARSVYVQAESQGLLDRLFEGQLAPMISHFAQHRE
                     LKAEEIARLKRLIEELEQGD"
     gene            3381..4199
                     /locus_tag="PLH31_02470"
     CDS             3381..4199
                     /locus_tag="PLH31_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQR88141.1"
                     /translation="MTVSAAESDVFAPASIAPQFVQARLTGVSVPGYPGGVLPETMAD
                     GYAVQDIAIDSWPDELVGWKVGYVPPQHQARLGAERLAGCIFKKNLWEAGAAPTPFRA
                     IEGGFTAVEAEFIIRLGKDAPEGKTQWTAEEAAEYVGELILGVEIAGSPLKTINVLGP
                     PIVASDFGNNDGQILGPVLENWRDIAWEDMAVETTINGVVVGKGTAASIPGSPLAALA
                     FLLGHVASRGRPLKKEMLVTTGASTGIHDVVAGDAATVSFGALGQVECVAVPAK"
     gene            4233..>5002
                     /locus_tag="PLH31_02475"
     CDS             4233..>5002
                     /locus_tag="PLH31_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88142.1"
                     /translation="MVIPKAPEPSEKIGRYRWVIVSLLFAAMVINYVDRQTIGILKSD
                     LSKEFGWSEGDYADLVFYFQLSYAVAYLAWGKIMDKIGARWGFGIAFAIWQIAHIAHA
                     GARGLNGFIFARMGLGIGEAGGFPGGIKAVAEWFPKKERALATGIFNAGTNIGAIVTP
                     LVVPGIVLAWGWQMAFIVTGVAGLIWLPIWLIMYRTPRQHKKIGAAELAHIEQDEADP
                     VEKIPWIKLLTKRETWAYSLGKFLIDPIWWMFLFWLPD"
BASE COUNT          922 a         1623 c         1564 g          893 t
ORIGIN      
        1 ggatctggat cgcattcttg agcgtggcgt gacgcccgtc gcggccgtgc atggcttggt
       61 ccgtcgcgac gccgccgggc agatatccag gaccccaccg catccgggcg tggcggacgc
      121 acgcgtgcta ctgcgtgagc tggcgcgatg cgacaaggac cttcagtttg aggacaaggg
      181 cgtcagcgtc accctgcact atcgaaacgc gccggccgct ggcgaagccg tgatcgaagc
      241 tgccgagcgt atcgcgcgca ccacagggct ggtgcttcag ctgggcgaca tggtcgttga
      301 gctgcgaacg ccaggccagg acaagggcca gtccgtcgcc gcctttctgc gcgagcaccc
      361 ctttaccgga tccaccccaa tcttcgtcgg cgacgacctg accgacgaag atggctttgc
      421 cgccgctctg caactgggcg gctacggcgt tctggtcggc gagatgcgcg cgacctccgc
      481 tgcctaccgt ttgaaggaca cggccgccgt ttcggcctgg ctggagggcg tggcgacgct
      541 gatgcccgca tgagccggct gatcgtcgtc tccaatcggg tcaacccgcc tccaagtcca
      601 gaaaagggcg atggcgacgg cagcgtcggc ggactggcaa tggctctggc tgcggcgttg
      661 cgcgagtatt ccgggatctg gtttggctgg agcggaaaga cgacgccgca gttcacgggc
      721 cagcttgcga tgcagcgggt cgatggcgtg accacagcaa cggtggatct cgaggacggc
      781 gactatcagg aatattacaa cggctacgcc aacaagaccc tgtggccgct ctttcactac
      841 cgtgtcgacc tgaccgccta tgatcgctcg ttcggcgagg gctatgatcg ggtcaacaag
      901 cgtttcgccg agaccctgag gcccttgatc gaacccgatg acatcatctg ggtccacgac
      961 taccacctga tcccgatggc gcgggagctg cgccggatgg gcgtgaccaa ccgtatcggc
     1021 ttcttcctgc acatcccgtg gcccgctcac cagctggtgg tcaccctgcc ccgccaccgg
     1081 ccgctggtgg aggcgatgtt cgactacgac ctgataggct tccagaccga ggaatcgcgt
     1141 cacgccttcg agggctatgt cacctccgag gtcgacggct cggtcgcgcc gaacggcgag
     1201 ttgacggcgt ttggcaagac cacgcgcgcg gccaccttcc cgatcggcat cgacgccgag
     1261 gacttcgccc ggatcatcga aagcccccgc gcccgcaagg tctatgaccg catgctggcc
     1321 aacagcgtgt tccgcaagat ggtcgtgggc gtcgatcgcc tggactattc caagggtctg
     1381 gaagagcggc tgatcggctt cgagcgcttc ctgcacgaca atcccgacat gcgccgcgag
     1441 gtcatgctgc tgcagatcgc acccctgtcg cgcgatgagg tcgaggccta tcaggacctg
     1501 cggtcgcgac tggacggttt gatcggccgg atcaacggcg cctacgccga gatggacttc
     1561 acgccgatcc gctacgtgaa caagtcctac cgtagggacg agcttgcggg gatctatcgc
     1621 gcggccaagg tcggcctggt gacgcccctg cgcgatggca tgaacctggt ggccaaggag
     1681 tatgtcgcct cacaggaccc tgaggatccc ggtgtcctga tcctgtcgcg cttcgccggc
     1741 gcggcgcgac agctgtcgga cgccctgatc gtcaatccca acagtcctga agaaatctct
     1801 gacgccctga gccgggccct ggcgatgggg ctgcccgagc gcaaacggcg ctgggagact
     1861 ctgttcgaca atgtgcaacg caaggatgtc acggcctggc gcgatgattt cgtcggcgct
     1921 ctcaccgcgt cgacccaaga aattcagccc gttaccccac ccatgtccct gcaggaggcc
     1981 tctacttcgg cctatgtcga ggcaagggcg gctaagatcc tgcctgcgac ggcaacggaa
     2041 aaccggcgac aatcctagcc tcgcgcctgc tgaagcacgc cgctgcggat gatcggcaca
     2101 aggaaggtgg tcagcaggac caggttcgga atggcgttgt tcccgtagcc agtaaggatc
     2161 gacatcgcag tgtcgatcac aaaccagacc atcacgcctc ccagcagaag gcgccagatg
     2221 ggcgtaccgc gttcacccag caggatcgct gcccggatcg tggccgccgc ggttatggcc
     2281 cagccgagag tcaccgcacc catcactgcc aggctgaagc gaatctgggc attcagatcc
     2341 agctccatcg ggccattcag aatatcgaag atcagccggg tcggaccgct ggtgacctcc
     2401 agaccgcccc cgaccagtac ggcgccaaac acgccgaccg ccccgcacca aaccgccaac
     2461 caattgcgcc agaagcctga catggccttc tcccaaattg tgatgacaga gccttggtcg
     2521 acggggcaag cgcgcgcaat tacccgtcag gttatggcct ttggaacagg cgggcaggcc
     2581 agcgctccct gacgccgacc tcagtcgccc tgttcgagct cctcaatcag gcgctttagt
     2641 cgggcgatct cctcagcctt cagctcacga tgctgggcaa aatggctgat catgggggcc
     2701 aactggcctt cgaacaggcg atccagaagc ccctggctct cggcctggac atagacgctt
     2761 cgcgcgataa gcggacggta gccatgcttg ccatcggcgc gctcggactt gatggccttt
     2821 ttcttcagca gccgattgat cagcgttttg accgtggctt cgccccaggt ctggctggcg
     2881 ccgacttcag ccaccaactc gtcggctgtc aggggctcac gccgccacag cgcctccatg
     2941 acatgacttt cagccgaagt gatatgcata gctcattaca tccgtaatac cacacgacgc
     3001 tatacccgtg ctgcagcctg tcaagcgatg gcgacgatca agcccaaaca ggcgttctgc
     3061 atccgcgcgc cagcagcagc ggctcagggt caatcgattg cccattgaac ccagagacaa
     3121 gccgccgtgc gggcgatctg gcgagcatga tcttcagtga cgtcgcgaac taccccgatc
     3181 aaggttagcg gcgcgcctgt cggatcaatc tctcccgccc ttttggacgg cagggcgccc
     3241 ccaaacgggg cgatcgcaac aaggtgtcga acgtagcgac cacaaccgcc cggcctcatt
     3301 gtcaatgaca ccggtttcca ttagagacga cgcagacccg caaaacgggc tatcgggtta
     3361 gtgtagggag gattagcggc gtgaccgttt ctgcggccga gagcgatgtg ttcgcaccgg
     3421 cgtcaatagc cccccaattc gtgcaggcgc gcctgactgg cgtctccgtt cccggctatc
     3481 cgggcggcgt gctccccgag accatggccg acggctacgc cgttcaggac atcgccatcg
     3541 attcttggcc cgacgaactg gtcggctgga aggtcggcta tgtgccgcct cagcatcagg
     3601 cccgcctggg cgccgagcgt ctggccggtt gcatcttcaa gaagaaccta tgggaagccg
     3661 gggccgcgcc gacgccgttc cgcgccattg agggcggctt caccgctgtt gaggccgagt
     3721 tcatcatccg cctgggcaag gacgcgcccg agggcaagac gcaatggacc gccgaggaag
     3781 ccgccgagta tgtgggcgag ctgatcctcg gcgtcgagat cgccggcagc ccgctgaaga
     3841 ccatcaatgt gctgggaccg cccatcgtgg cctcggactt tggcaacaat gacggccaga
     3901 tcctgggtcc ggtgctggaa aactggcgcg acatcgcctg ggaagacatg gccgtcgaga
     3961 ccacgatcaa cggcgtcgtc gtcggcaagg gcacggcggc ctcgatcccc ggttccccgc
     4021 tggcggccct ggccttcctg ctgggtcacg tcgcctcgcg cggccgtccg ctgaagaagg
     4081 agatgctggt caccaccggc gccagcaccg gcatccacga tgtggttgcg ggcgacgcgg
     4141 caacggttag cttcggcgcc ttgggacagg tggagtgcgt cgccgtcccg gcaaaataag
     4201 aaacgacaat ccagggtaag gaagacgagt ccatggtcat tcccaaagcc cccgaaccct
     4261 ccgaaaagat cggtcggtat cggtgggtga tcgtcagcct gctgtttgca gcgatggtca
     4321 tcaactatgt tgatcggcag acgatcggca ttctgaagtc cgatctctcc aaggaatttg
     4381 gctggagcga gggcgactat gctgacctcg tgttctattt ccagctgtcc tatgccgtgg
     4441 cctatctggc ctggggcaag atcatggaca agatcggggc ccgctggggc ttcggtatcg
     4501 ccttcgccat ctggcagatc gcccacatcg cccacgccgg cgcgcggggc ctgaacggct
     4561 tcattttcgc ccgcatgggc ctgggcattg gtgaggccgg cggcttcccg ggcggcatca
     4621 aggcggtggc cgagtggttc cccaagaagg aacgcgccct cgccaccggc atcttcaacg
     4681 ccggcaccaa tatcggcgcg atcgtcaccc cgctggtggt gccgggcatc gtgctggcct
     4741 ggggctggca gatggccttc atcgtcaccg gcgtggccgg cctgatctgg ctgcccatct
     4801 ggctgatcat gtaccgcacg ccccgccagc ataagaagat cggcgccgcc gagctggcgc
     4861 acatcgagca ggacgaggcc gatccggtgg agaaaatccc ctggatcaag ctgctgacca
     4921 agcgcgaaac ttgggcctat tcgctgggca agttcctgat cgacccgatc tggtggatgt
     4981 tcctgttctg gttgcccgac tt
//
LOCUS       DAOMEO010000067         4998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2110_length_4998_cov_2.387214, whole genome shotgun sequence.
ACCESSION   DAOMEO010000067 DAOMEO010000000
VERSION     DAOMEO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_4998_cov_2.387214"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLH31_02480"
     CDS             complement(<1..609)
                     /locus_tag="PLH31_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HQR88143.1"
                     /translation="MSVNLTPDLAHVETWLFDLDNTLYPAECEYMALIEGKMTDFMER
                     TTGLPREEARAIQKRYYHEHGTTLAGLMAHHGIAPKAFLDDVHDVSMDRLVPDPVLRA
                     SIDRLPGRRLIFTNGSLGHAERVLGHLGLDHLFEDVFAIETADYLPKPAMATFEKIVS
                     RHALAAPLTAFFEDSEKNLAPAAVLGMTTVLVGAHAAASTADF"
     gene            complement(606..1520)
                     /gene="argB"
                     /locus_tag="PLH31_02485"
     CDS             complement(606..1520)
                     /gene="argB"
                     /locus_tag="PLH31_02485"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQR88144.1"
                     /translation="MTDAAEEAGWATAKTLAEALPYIQIYDRETVVIKYGGHAMGQEQ
                     VAKLFAADAVLLKLLGVHPVVVHGGGPQISRMLERAGVKSTFIDGLRVTDEATMEVAE
                     MVLSGAINKEIANWITLAGAEADVRGVGLSGKDARMITAEKVTRTKRDPGSNIEQVVD
                     LGFVGEPTKVDPHIIQALLSSETDYIPVIAPIGVSEAGQTFNINADTVAGALAGALKA
                     KRMLMLTDIAGVLDADGQLIRQMTVEEARGLIESGVASGGMIPKLENAIHAVESGVEA
                     VVILDGRRPHAMLVELFSEHGAGTLISK"
     gene            complement(1602..1877)
                     /locus_tag="PLH31_02490"
     CDS             complement(1602..1877)
                     /locus_tag="PLH31_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HQR88145.1"
                     /translation="MAASSAAVAVGVAAALLSITSFAPQMLKIWKERDASSVSLRTYL
                     ITVTGFVCWTLYGVMIQAWPVVASNLACLAMSSGVLVMKWRFDRKAA"
     gene            complement(1880..2344)
                     /locus_tag="PLH31_02495"
     CDS             complement(1880..2344)
                     /locus_tag="PLH31_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88146.1"
                     /translation="MNDQPLGVLIIEDEVLLATELEFLLEEAGCCSVGHAMSSHEAVT
                     LARDLKPDLALIDVHLCDGPTGVDVARRIQDDCGAVALFMTANVKRLPEDFAGACGVI
                     GKPYSSHGVKTALGYLSICMREGHAPGPPPIALTLSPQYLALWGLLDMPRAG"
     gene            complement(2366..3472)
                     /locus_tag="PLH31_02500"
     CDS             complement(2366..3472)
                     /locus_tag="PLH31_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR88147.1"
                     /translation="MTDESQSADAAGASRPGSRAQDAFAAAFQATRVAMIVTDADQPD
                     NPILFANDAFLRLTGYGREEVIGRNCRFLQGADTDPVQVARIRAAIEAGQDIAAEVVN
                     YRKDGSTFWNALYVSPVRGEDGAVSYFFATQLDVSDKRKAEQTLTDSRHDLEQLVAER
                     TSELTQALEQKTALLHEVDHRVKNNLRLISSLLLLQNRRVSDPAVKAALRGMLDRVSA
                     IATVHRRLFQSDDVARFDVSAFVRDLVADVIGASGREDIAFSLDLERVDVAASKAAPL
                     ALVINELLSNTLRHAFPGGRPGRIFVGINRIDEAFRIEITDDGVGVDSSTRLSGFGFT
                     IVQLLCQQLKARFESTDASPGTRVVIDLPVDVAP"
     gene            complement(3673..4725)
                     /locus_tag="PLH31_02505"
     CDS             complement(3673..4725)
                     /locus_tag="PLH31_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR88148.1"
                     /translation="MTRRFEKPTRRGVLGGASAGAAALAAPGLIAGQAQAQGVRPLTL
                     LNVSYDPTRELYKDINAAYARYWKEKVGQTLTINQSHGGSGKQARSVIDGLEADVVTL
                     ALAYDIDEIATRSRLLPANWQSRLPNNSTPYSSTIVFLVRKGNPRKIKDWSDLVKPGV
                     GVITPNPKTSGGARWNYLAAWAWALKQPGGNPAKAQAYVAALFKNVPILDTGARGATT
                     TFTQRGIGDVFLSWENEAYLAQEELPGKFDIVYPSLSILAEPPVALVDRNVDRRKTRL
                     AAQGYLNFLYSPLAQNLIAKHHYRPRNPVVAAKHAGKFKTLPLVTIDDTFGGWKTAQA
                     THFADEGVFDRIYQPR"
     gene            complement(4754..>4998)
                     /locus_tag="PLH31_02510"
     CDS             complement(4754..>4998)
                     /locus_tag="PLH31_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88149.1"
                     /translation="AGGPGNPTLPGAIRGGEQNIISLGVNWYLNSVVMVSGAYRNVEV
                     RRLSPGGTAFGAATPAAGAQVGQDLDIWSLRTQYVF"
BASE COUNT          826 a         1588 c         1711 g          873 t
ORIGIN      
        1 aaagtcggcg gtcgaggcgg ccgcatgggc gccgaccagc acggtggtca tgcccagaac
       61 ggcggcgggg gccaggttct tctcgctgtc ctcgaagaag gcggtgagcg gcgcggccag
      121 ggcatggcgc gagacgatct tctcgaacgt ggccatggcc ggcttgggca gatagtcggc
      181 cgtctcgatg gcgaagacgt cctcgaacag atggtccagg cccagatggc ccagcacgcg
      241 ctcggcatgg cccagcgagc cgttggtgaa gatcagccgg cggccgggca ggcggtcgat
      301 cgaggcgcgc agaaccggat cggggaccag acgatccatc gagacgtcgt gcacgtcgtc
      361 gaggaaggcc ttgggcgcga tgccgtgatg ggccatcagg ccggccaggg tggtgccgtg
      421 ctcgtggtag tagcgcttct ggatggcccg ggcctcttca cggggcaggc cggtggtccg
      481 ctccatgaag tcggtcatct tgccctcgat cagggccatg tattcgcact cggcgggata
      541 cagggtgttg tcgagatcga agagccaggt ctcgacgtgt gccagatcgg gggtgaggtt
      601 gacgctcatt tcgagatcag cgtgccggcg ccgtgctcgc tgaacagttc caccagcatg
      661 gcgtgcggac ggcggccatc caggatgacc acggcctcga cgcccgattc caccgcgtga
      721 atggcgtttt ccagcttggg gatcatgccg ccgctggcga cgccgctttc gatcaggccg
      781 cgggcttcct cgaccgtcat ctggcggatc agctggccgt cggcgtcaag caccccggcg
      841 atgtcggtca gcatcagcat ccgctttgcc ttcagggcgc cggcgagcgc tccggccacc
      901 gtgtcggcgt tgatgttgaa ggtctggccg gcctcggaga cgccgatcgg ggcgattacc
      961 gggatgtagt cggtttccga cgacagcagg gcctggatga tgtgcggatc aaccttggtc
     1021 ggctcgccca cgaagcccag gtcgacgacc tgctcgatat tgctgcctgg gtcgcgcttg
     1081 gtgcgggtca ccttctcggc ggtgatcatc cgggcgtcct tgcccgacag gcccacgccg
     1141 cggacgtcgg cctcggcgcc ggccagggtg atccagttgg cgatttcctt gttgatggcg
     1201 cccgacagca ccatctcggc cacttccatg gtcgcttcgt cggtgacgcg caggccgtcg
     1261 atgaaggtcg acttgacgcc agcccgctcc agcatccggc tgatctgcgg accgccgccg
     1321 tgcaccacta ccggatggac gcccagcagc ttcagcagca cggcgtcggc ggcgaacagc
     1381 ttggcgacct gttcctggcc catggcgtgg ccgccgtatt tgatcaccac cgtctcgcgg
     1441 tcgtagatct ggatgtaggg cagagcctcg gccagggtct tggcggtggc ccagcccgct
     1501 tcctcggcgg cgtcggtcaa ggttgcggat cctgtgcgaa aagggcgggt ctcgatagcg
     1561 gaccccgccc cccctgtcac tcaaaagagg acgttgaagg ctcaggccgc cttgcggtcg
     1621 aagcgccatt tcatgaccag cacgcccgac gacatggcca ggcaggcgag gttggaggct
     1681 acgaccggcc aggcctggat catgacgccg tagagcgtcc agcagacaaa gccggtaacg
     1741 gtgatgaggt aggtgcgtag cgacaccgac gaggcgtcgc gctctttcca gatcttgagc
     1801 atctgggggg cgaaactggt gatcgacagc agcgcggcgg cgacgccgac cgcgaccgcc
     1861 gcggacgagg ccgccatggt cagccggccc tgggcatgtc gagaaggccc cataaagcca
     1921 gatattgcgg cgacagggtc agggcgatcg gcggcgggcc gggcgcatgc ccctcgcgca
     1981 tgcagatgct cagatagccc agcgcagtct tcacgccatg actcgaatag ggcttgccga
     2041 tcacgccgca cgcaccggcg aaatcctcgg gcaggcgttt gacgttggcg gtcatgaaca
     2101 gggccaccgc cccgcaatcg tcctggatgc gccgcgccac gtccaccccc gtcggaccat
     2161 cgcaaagatg gacgtcaatc agggccagat cgggcttgag atcccgcgcc agcgtcaccg
     2221 cctcgtgtga actcatcgca tggcccaccg aacagcaccc ggcctcctcc agcaggaact
     2281 ccagttccgt ggcgagcaga acctcgtctt cgatgatcag aacgcccagg ggttgatcgt
     2341 tcatggcgca cccttgccct tgcgactaag gcgcgacgtc aacgggtagg tcgatgacaa
     2401 cgcgcgtacc gggcgaggcg tccgtgcttt cgaacctggc cttcaactgc tggcacagca
     2461 gttgaacgat cgtgaacccg aaacccgaca acctcgtcga actgtccaca ccaacgccgt
     2521 cgtcggtgat ttcgatccgg aaggcctcgt cgattcggtt tatgccgacg aaaatccgtc
     2581 cgggacgccc gcccgggaag gcgtgtcgca gggtgttgga cagcagctcg ttgatcacca
     2641 gggccagggg agcggccttg gaggcggcca catccacccg ttccagatcc aggctgaagg
     2701 cgatgtcttc gcggcctgac gcgccaatga cgtcagccac cagatcgcgg acgaaggccg
     2761 agacatcgaa gcgcgcgaca tcgtcgctct ggaacagccg gcgatgcacg gtggcgatgg
     2821 cgctgacccg atccagcatg ccgcgcaggg cggccttgac cgccggatcc gagactcgcc
     2881 ggttctgcag cagcagcagg gacgagatga gccggaggtt gttcttgacc cgatggtcga
     2941 cctcgtgaag cagggcggtc ttctgctcca gggcctgggt cagttcgctg gtccgttcgg
     3001 ccaccagctg ttccagatca tgacgcgagt cggtcagggt ctgctcggcc ttcctcttgt
     3061 cgctgacgtc cagctgtgtg gcgaagaaat agctgacggc gccatcctca ccgcgcaccg
     3121 ggctgacata gagggcgttc cagaacgttg agccgtcctt gcgatagttc acaacctcgg
     3181 cggcgatgtc ctggccggct tcaatggcgg cgcggattct ggccacctgg acggggtcgg
     3241 tgtcggcgcc ctgcaagaag cggcaattgc ggccgatgac ctcttcgcgg ccatagccgg
     3301 tgagccgcag aaaggcgtca ttggcgaaga ggatgggatt gtcgggctgg tcggcgtcgg
     3361 taacgatcat cgccacgcgt gtggcctgaa aggctgcggc gaaggcgtcc tgggccctgg
     3421 atccgggcct ggatgcgccc gcggcgtctg ccgattggga ttcgtccgtc atcagatggg
     3481 ctggcccggc atgtcgtctc ccatgcggcg ctaacgcgct ctacaactgt cggttcccgc
     3541 tgtgtcgagt ttccgacctc tgtggaagca tgccccaaaa ctggcgcggc gtgaatccgc
     3601 atgggaatca ggccgcgccc gcgagacgac aagcggagga tgggcgtccc gcgatcccga
     3661 tcctgggccg acctatctgg gctggtagat cctgtcgaac accccctcgt cggcgaagtg
     3721 cgtggcctgg gccgtcttcc acccgccgaa ggtgtcgtcg atggtgacca gcggcagggt
     3781 cttgaacttg ccggcatgct tggccgccac caccgggttg cgcggccgat agtggtgctt
     3841 ggcgatcagg ttctgggcca gggggctgta gaggaagttc agatagccct gagcagccag
     3901 ccgggtcttg cgacggtcga cattgcgatc caccagcgcc accggcggct cggcaaggat
     3961 cgacagcgac ggatagacga tgtcgaactt ccccggcagc tcctcctgcg ccagataggc
     4021 ctcgttttcc caggacagga acacatcgcc gatgccgcgc tgggtgaagg tggtggtcgc
     4081 gccgcgcgcg ccggtgtcaa ggatcggcac attcttgaac agcgcggcga cataggcctg
     4141 ggccttggcc ggattgccgc ccggctgctt cagcgcccag gcccaggcgg ccagatagtt
     4201 ccagcgcgcg ccgcccgagg tcttgggatt gggcgtgatc accccgacgc ccggcttcac
     4261 cagatcgctc cagtccttga tcttcctggg attgcccttg cggaccagga aaacgatcgt
     4321 cgagctgtag ggcgtggaat tattgggcag gcgtgattgc cagttggccg gcagcaaccg
     4381 ggagcgggtc gcgatctcgt cgatgtcgta ggccagcgcc agggtgacca catcggcctc
     4441 caggccgtcg atcaccgagc gggcctgctt gcccgatccg ccatggctct ggttgatggt
     4501 cagggtctgg ccgacctttt ccttccagta cctggcgtag gcggcgttga tgtccttgta
     4561 gagctcgcgc gtcggatcgt agctgacgtt cagcagggtc agcggcctga cgccctgagc
     4621 ctgggcctga cccgcgatca gtccgggcgc ggcaagggcc gccgcgcccg ctgatgctcc
     4681 gccaaggacg ccccgacgcg tgggtttttc aaaacgacgg gtcatggggc gtccttggct
     4741 tggtggggtg gtgttagaac acgtactggg ttcgcaggga ccagatatcc agatcctggc
     4801 cgacctgggc gccggcggcg ggcgtcgcgg cgccgaaggc cgtcccgccc ggcgacaggc
     4861 ggcggacctc gacattgcga taggcgcccg acaccatgac gacgctgttg aggtaccagt
     4921 tcacgcccag cgagatgatg ttctgctcgc cgccgcggat cgcgccgggc agggtcggat
     4981 tgccggggcc gccggcca
//
LOCUS       DAOMEO010000068         4994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2114_length_4994_cov_2.711885, whole genome shotgun sequence.
ACCESSION   DAOMEO010000068 DAOMEO010000000
VERSION     DAOMEO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_4994_cov_2.711885"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1196
                     /locus_tag="PLH31_02515"
     CDS             99..1196
                     /locus_tag="PLH31_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88150.1"
                     /translation="MVRPDDLTPTGSDLQNSYRVGEQVAAIYARLDFEGQAAAWPFFG
                     NVGLRLVESRTHVEGARMELVDGAATIRPVSFDGAHEILLPSLNMAFDVAEDAVVRLA
                     VSRTMTRPSLADLRASTVPASSLVSAIYTDGQAAIDDAGPGLVFAGVGGNPALTPYVS
                     DNIDFSYEVTRRWSSLSLAIFHKTVNDFIQTVEAPEILTFETLNGSPVQAEVRMARPR
                     NLGRAVVNGLEIGVHRRLADQLGVWSSATWTHSRVGDRRLTGVSDWSWSVSPYVERGP
                     LALNLSWSWRSAFTSEADLQGGGVSNFTVGAAGYLDAQVRYEIASNAQLVIAGSNLTD
                     THDLAYEGSKSRLLQLGSSGRSFSMRLNWRW"
     gene            2201..2857
                     /locus_tag="PLH31_02520"
                     /pseudo
     CDS             2201..2857
                     /locus_tag="PLH31_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            2876..4867
                     /locus_tag="PLH31_02525"
     CDS             2876..4867
                     /locus_tag="PLH31_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR88151.1"
                     /translation="MSVSRDISDRKALEFEMMAARDAAREQAQRARLAEDIGGVCYWR
                     YDLESQDLDVSPKMFEIYGLERVERPRVEVFDDCIHPDERAWTKARIAERLKTGQADY
                     NVTSRVVRPDGEERYISGSSIIEMGPDGSPAVIMGTVRDITDERRSQADLAASESRYR
                     LLADNASDMITTFGPGGAIRFISPACYPILGYTPQEMIGRRIIEMTHPEDEAVIMAYY
                     ADLIAKGPSAISEPYQFRGRHKNGHWVWLEGQPKLFFDASTGKVVAIQDVARDITVRK
                     ALEAALELARAEAEAAAAVKAEFLSNMSHELRTPLTAVLGFSRLIEGQPELSPATRRF
                     VDRVSNAGKALLSTVNDILDFSKLEAGQVEIVVTSCNPRQLLEEAMDLFSGQAQEKGL
                     DLVMTGLDQLPAGLALAPDRIRQILLNLIGNAVKFTEGGRVVLDARWTARSRRLRISI
                     TDSGAGIPADRLDQLFKRFSQVDGSSTRRHGGTGLGLAICKGLVDAMGGQIGVSSEAG
                     QGSSFWFEIPADRASGAVAEVDGGTSVVPEGCRVLVVDDNPVNRDLVCAVIRAFGAEM
                     TEAPDGEAAVRLASNEPFDVILMDLRMPSMGGEAATRRIRQDSLNGNVPIIAFSADVG
                     GVLPTGVFDGVVPKPLDARVLVAEINRTLSVDGDIENAA"
BASE COUNT          913 a         1506 c         1587 g          988 t
ORIGIN      
        1 tcggcgatct tctagcggtg cgctccgcac cctgggttat cgctgactac gatcacctgc
       61 gcgcggcctt cctgattccc ggggaaggcg gcggcgtcat ggtcaggcct gacgatttga
      121 cgccgacagg ctctgacctt cagaactcct atcgtgtcgg agagcaggtg gcggcgatct
      181 acgcccgtct cgattttgaa ggccaggctg cggcatggcc ctttttcggc aatgtcgggc
      241 tccgcctggt agaaagccgc acccatgtcg aaggcgcgcg gatggagctc gtcgatggcg
      301 cggctacgat cagacctgta tcgtttgatg gcgcgcacga gattttactg cccagcctga
      361 acatggcgtt cgatgtcgcg gaggacgccg tagtgcgcct tgcggtgtcg cgcactatga
      421 cgcgtccctc gctggcagat ctgcgcgcct cgaccgtgcc ggccagcagc ctggtgtcag
      481 caatctatac cgatggtcag gcagccattg acgacgcagg gcctggcctc gtctttgctg
      541 gcgttggtgg aaacccggcc ctgaccccgt atgtatctga taatattgac ttttcatacg
      601 aggtaactcg tcgctggagc agtctcagcc ttgccatctt ccacaagacc gtcaacgact
      661 tcatccagac cgtcgaagcg cctgaaatcc tgaccttcga gacgctgaac ggctcccccg
      721 ttcaggcaga ggtgcgaatg gcgcggccgc gaaacctagg gcgtgctgtc gtcaacggcc
      781 ttgagatcgg ggttcatcgt cgcctggccg atcagttggg ggtctggagc agcgcgacct
      841 ggacccatag tcgggttggt gaccgacggc ttacgggtgt gtcggactgg tcctggtcgg
      901 tcagccccta tgtggagcgc ggcccgctgg cgctgaacct gtcatggtcg tggcggtcgg
      961 ccttcacgtc ggaggcggac ctgcagggcg gcggtgtctc gaacttcacc gttggcgcag
     1021 cgggctacct cgacgcccaa gtccgctatg agatcgcaag caacgcccaa ctggttatcg
     1081 caggctcgaa cctcacagac acgcatgacc tcgcttatga agggtcaaaa tcgcgtcttc
     1141 tgcagctcgg atcttcaggc cgatcgtttt cgatgcggtt gaattggcgc tggtaggcct
     1201 tggtcctctc gcgatgcgcc agaaagccta tctgttaccg acgccaaacc ccgagggcgc
     1261 ggtgcagctt aagcccagcg gcgtgcagaa ttgggtttgg cgcgaccgtg gccgttacac
     1321 gcttgttccg ggctttcgat tatccagagc ctttctgacg ccgtcctcgg ctctcctggt
     1381 gccggagcaa tctcaaaggt ctggagatgg cgaagcgcta tcccatgcag gaggtcgctt
     1441 ttgtcgcgat gggcgcagct tttacagggt ccgaaagtgg tcgcaatgct gaccagcgat
     1501 aggccgcaat ccgaagtggc tccggcattc gctccgtgtg ccaagcggct gacgcccggt
     1561 cgtagcgagc gcctgagaat tttggaacgc tgcgtcactc tgccacaggc actttaacca
     1621 tacccctatc tctttcatcc aacgatcatg aaaacgggga ataggcggca tggacgatcg
     1681 cgagaggcga aggctggagg ccttgcgagc ctacaacgtg ctgaacaccg aggcggacga
     1741 cgcgattgat cggctggcgg gactggccgg gaacctcttc tcgacaccga tttcgctcgt
     1801 ctcgctgatt gacgagcatc gtcagtggtt caaggccagg gttggccttg aggcttcaga
     1861 aaccccgcgc gaccaggcct tttgcgctca tgccatcacc atgggacccg acatggttat
     1921 ggttgtcgaa gacgccaggc tggatcctcg cttcgcgacc aatcccttgg tcacgggcgc
     1981 gccgggtatt cgtttctacg cgggcgcgac gctgaccaca aaggatggtc ataatctggg
     2041 cacgctctgt gtcattgacg ataagccgag gccgcgtcca agccccgccg agctggaacg
     2101 gcttcagcta ctggcgcgta tcgtggtcga cgagttcgag ctggcgcgtg ctcatcgcca
     2161 ggctcgcgaa aagcaacgcc ttcttgaaat cgctgagacc atgtcgggtg taggccactg
     2221 gcgtttggac ctgggcgcca acaagccaac atggtccgat acggtctatg cgatccacgg
     2281 cgtcgatcgc gcaagcttcg atccggggct cgacgatgcc gtcgccttct atcaccccga
     2341 tgatcgcgag atggtgttgg gccatctggc ctccgcgata gagaccgggt ccggttttga
     2401 cttccaactc aggttggtcc gcgctgacgg ctcgttgcgc cacgttatct cgcgcggcgt
     2461 ttgcgaaata gacgacctgg gccgcccaac tgcgatcgtt gggttgttcc aagatataac
     2521 tgagaccgtc gagacgatcg caacgctgca tcgccgcgag cagcattatc ggctcgtgac
     2581 cgaaaactcg gccgacgtca tcacgcgcta tgatttcgac ggcgacggta gtttcatttc
     2641 gcccgccatt gaaaagctgc tcggctacac agtcgcggaa accaaagaac tgaacctgtg
     2701 ggaggttctg catccaggcg atcgtgaacg tgtgacggaa gcgttcgggg cgatgtcgcg
     2761 cgggcttgac cagagcacgc ttcaacaccg ttcgcgccac aagcgcggcc attacatctg
     2821 ggtagaatca aacctgcaac tggtgcgtga cgcctccggc gcgccccggg aaatcgtgtc
     2881 ggtttcgcgt gatatctccg accgcaaagc cctggaattc gagatgatgg ccgcccgaga
     2941 tgcggcgcgg gaacaggctc agcgcgcgcg actggcggag gatatcggcg gggtttgcta
     3001 ctggcgctac gatctagagt cccaggacct ggatgtgtcg cccaagatgt tcgagatcta
     3061 cgggctggaa cgcgtcgagc gtccgcgcgt cgaggtcttc gacgactgta tccatcccga
     3121 cgagcgggcc tggactaagg ccaggatcgc cgagcgactg aagaccggcc aggcagatta
     3181 caatgtcacc agtcgcgttg tgcgaccgga tggcgaggaa cgttacattt cgggtagttc
     3241 gatcatcgag atgggccccg atggctcgcc ggccgtcatc atgggcacgg tccgggacat
     3301 cactgacgaa cgccggagcc aggccgacct ggcggccagc gaaagccgct accgtctgct
     3361 ggccgataac gccagcgaca tgatcacgac gtttggccca ggcggcgcta ttcgattcat
     3421 ctcgccagct tgctacccaa tcctgggcta cacgccccag gagatgatcg gccgccggat
     3481 catcgaaatg acccatcccg aggatgaggc ggtgattatg gcttattatg ccgacttgat
     3541 cgccaaaggg ccttccgcga tctccgaacc ctaccagttt cggggacggc acaagaacgg
     3601 ccactgggtg tggctggaag ggcagcccaa gctgtttttt gacgcgtcca ccggcaaggt
     3661 ggtcgccatc caggacgtcg cgcgggacat caccgtccgc aaggcgctgg aagcggccct
     3721 ggaactggcc cgcgctgagg cggaggcggc cgcagcggtg aaggccgaat tcctgtcgaa
     3781 catgagccat gagctgcgca cgccattgac ggctgtgctg ggcttttcgc gcttgattga
     3841 gggtcaaccc gagctgtcgc cagccacccg tcgcttcgtg gatcgggtct ccaacgctgg
     3901 caaggcgctg ctatcgacag tcaatgacat tctggacttc tccaagcttg aggccggtca
     3961 ggtcgagatc gtcgtcacct cctgtaatcc gcgccagctt ctggaagagg ccatggatct
     4021 cttctcgggc caggcccagg agaagggcct ggatctggtc atgaccggcc tggatcagct
     4081 gcctgcaggg ctagccctgg cccccgaccg catccgtcag atcttgctga acctgatcgg
     4141 caacgcggtg aagttcaccg agggtggtcg ggtggtgctg gacgcaaggt ggacggcacg
     4201 atcccgccgc cttcgcatat caatcaccga cagcggcgcg ggcatccctg ccgaccgcct
     4261 ggatcagctg ttcaagaggt tctcgcaggt tgatggctcc tcaacgcggc gacatggcgg
     4321 gacaggcctc ggtcttgcga tctgcaaggg ccttgttgac gccatgggcg gccagatagg
     4381 ggtttcaagc gaggctggcc agggctccag cttctggttc gagattccgg ccgacagggc
     4441 ctcgggcgcc gtagccgagg ttgatggtgg gacatcggtc gtccctgagg gctgccgtgt
     4501 gctggtggtt gacgacaacc cggtcaatcg tgacctggtt tgcgcggtga tacgggcttt
     4561 cggcgctgaa atgaccgagg cgcccgacgg tgaagcggcg gtccggctgg ccagcaacga
     4621 gcctttcgac gtgatcctga tggacctccg catgccgagc atggggggag aggccgcaac
     4681 ccggcggatc cggcaggata gcctcaatgg caacgtcccg atcatcgcct tctcggccga
     4741 tgtgggcggc gtcttgccaa ccggtgtctt cgacggcgtg gtccccaaac cactggatgc
     4801 ccgcgtgctt gtcgcggaga tcaaccgcac cctgtctgtc gatggggata tcgaaaatgc
     4861 cgcctaaagc gcgcgcgcca cgaatcttca tcgcagaaga cgaccgcaac gtgctggaac
     4921 tgctgaccac ccggcttggt ctcgcaggct atgaaaccag tttcggccgc gacggctggg
     4981 aggcgcttga aggc
//
LOCUS       DAOMEO010000069         4970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2121_length_4970_cov_2.693591, whole genome shotgun sequence.
ACCESSION   DAOMEO010000069 DAOMEO010000000
VERSION     DAOMEO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_4970_cov_2.693591"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLH31_02530"
     CDS             complement(<1..1254)
                     /locus_tag="PLH31_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88152.1"
                     /translation="MSSTRTSAGVTPEQLATLSHEFRTPLNGVLGMARLLEGTRLTGE
                     QRAYVTALRESGDHLLALVNDVLDFARLGATAIELAPAPVDIEGLLRQVAELMSPRAH
                     EKGVEIAWAVSPGLPTILADEGRLRQILLNYAGNAVKFTQKGGVLLTATAAGEGRVRF
                     SVADTGPGVAPEARAKIFEAFVQTDASHRTQLGGAGLGLAIVARLARAMDGQAGVGGE
                     VGQGADFWFEAPFAVAAPATPERPLTGQTVAVASPNPIVRIATARQIEAAGGRAIVAA
                     DTAAALAAAPADAVLLIDAALAGPRGALKPPVDRRAVVLLTPDQRGWIDRLKTAGFSG
                     YLIKPLRAASIVAQVLQATAHSPAPAAERHAEDDRIAEAAAPGVRVLLAEDNPINALL
                     ARTLLEREGCKVDRVADGEQDVAALS"
     gene            complement(1306..2856)
                     /locus_tag="PLH31_02535"
     CDS             complement(1306..2856)
                     /locus_tag="PLH31_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQR88153.1"
                     /translation="MAARVVTVAFEGVEARRVDVEVQLTSGQQIFVIVGLADKAVAES
                     RERVRGAFAGLGLSLPGKRIVANLAPADMPKEGTHFDLPIALAVMAAIGVIPLDALDG
                     WAAMGELSLDGRIVPAAGALPAAMAAGAMGLGLICPEASGPEAAWAGGTRILAPRSLV
                     ALINHFRGSQVLSAPSPGPVIEGERAKDLRDVKGQEHAKRALEIAAAGGHNLLFVGPP
                     GSGKSMLAQRLPGLLPPLTSTELLQTSMVHSVAGLIARGALTRARPFRSPHHSASMAA
                     LTGGGHKAKPGEVSLAHNGVLFLDELPEFGVQALDSLRGPLETGEVMVARANAHVRYP
                     AKVQLVAAMNPCRCGHGGAGRGYCGKAPRCQRDYQGRVSGPLMDRIDLSVDMPAVTAA
                     DLALPPPSEGSAEFAARVARARQLQIDRAEAHESLEALNGRAEGAFLEKIVAIDEAGR
                     SLLARAAEAGKISARGWTRVLRLSRTIADLEGADGVRRVHVAEALAHRRSATPGEDVA
                     PSFGQPVF"
     gene            complement(2970..>3610)
                     /gene="gshB"
                     /locus_tag="PLH31_02540"
     CDS             complement(2970..>3610)
                     /gene="gshB"
                     /locus_tag="PLH31_02540"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQR88154.1"
                     /translation="LEKVHPHTLVVNNPAEVRNAPEKLFVTDFPGVQPPTLITSDYEA
                     IYDFRARHGDIVLKPLYGGGGSGVARLLADDPNLDALLELHAMIGREQVIAQKFVPAV
                     SKGDKRVLLVDGEPVGAINRVPAAGQVRSNLRVGGRAEAVELTARDLELCAIIAPELK
                     RRGLLFVGIDVIGEYLTEINVTSPTGAQQLKRFGGADASAALWDRIEAIRAA"
     assembly_gap    3611..3620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3621..4030)
                     /locus_tag="PLH31_02545"
     CDS             complement(<3621..4030)
                     /locus_tag="PLH31_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQR88155.1"
                     /translation="MSLRVAVQMDPVHGINIDTDTTFQMMMEAQTRGHKLWFYTPDKL
                     SMEDGRVFARAQSLVLSSVKGAHAELGESLLLDMKDDVDVVLMRQDPPFDMAYITTTH
                     LLEMIQPKTLVVNDPFWVRNSPEKLLVLRFPSLTP"
     gene            complement(4111..4926)
                     /locus_tag="PLH31_02550"
     CDS             complement(4111..4926)
                     /locus_tag="PLH31_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQR88156.1"
                     /translation="MTREEAEAILTRAGQGPDDGFPLFEAALACAVHDDPDRDTGPVI
                     ALAQQAVERLAVRMQTESPEEAVAETLAGDLHLTGDLITYDHPDNADVIAIARRRKGL
                     PVALGVFYLHAARMTGVDLHGVDFPGHFLLRIETDEGPLALDPFGEGRVVLPSELSRR
                     ALRTGLMPDVADKLERLMAPIPDRAVLIRLQNNIFARAQQARDWLRAERSALRRALLN
                     PADHRPWLDVAAAREGQGALAGALQALTQAQILDGGAAIAARAARERVRLRLN"
BASE COUNT          792 a         1700 c         1746 g          722 t
ORIGIN      
        1 ggacagggcc gcgacgtcct gctcgccatc agcaacgcgg tcgaccttgc aaccttcgcg
       61 ctccagcagg gtgcgggcga gcagggcgtt gatcggatta tcctcggcca gcagcacccg
      121 cacacccggc gccgcggcct cggcgatccg gtcgtcttcg gcatgacgct cggcggcggg
      181 agcggggcta tgggcggtgg cctgcagcac ctgggcgacg atggaggcgg cgcgcagggg
      241 cttgatcaga tagccgctga agccggccgt cttcagccgg tcgatccagc cgcgctggtc
      301 aggggtcagc agcaccacgg cgcgacggtc gacggggggc ttcagcgcgc cgcgcggccc
      361 ggccagggcc gcgtcgatca gcagcacggc gtcggccggg gcggcggcga gggcggcggc
      421 cgtatcagcg gcgacgatgg cgcggccgcc ggcggcctcg atctggcggg cggtggcgat
      481 gcggacaatg gggttgggcg aggcgacggc caccgtttgg cccgtcaatg ggcgttcggg
      541 cgtggcgggc gcagcgaccg cgaacggcgc ctcgaaccag aagtcggcgc cctggccgac
      601 ctcgccgccg acgccggcct gcccgtccat ggcgcgggcc agacgggcga cgatggccag
      661 gcccaggcct gctccgccca gctgggtgcg gtggctggcg tcggtctgga cgaaggcctc
      721 gaagatcttg gcgcgggcct cgggcgcgac gcccggcccg gtgtcggcga cgctgaagcg
      781 cacgcggcct tcgccggcgg cggtggcggt cagcagcacg ccgccctttt gcgtgaactt
      841 cacggcgttg ccggcatagt tcagcaggat ctgccgcagg cggccctcat cggccaggat
      901 ggtcggcagg cccggcgaga cggcccaggc aatctcgacg cccttttcgt gggcgcgcgg
      961 gctcatcagc tcggccacct ggcgcagcag gccctcgata tcgaccggcg ccggggccag
     1021 ctcgatggcc gtggccccaa ggcgggcgaa gtccagcacg tcattgacca gcgccagcag
     1081 gtggtcgccg ctttcacgca gggccgtgac ataggcccgc tgctcgcccg tcaggcgtgt
     1141 gccctccaac agccgcgcca tgcccagcac gccattcagc ggcgtgcgaa attcatggct
     1201 caacgtggcc aattgctcgg gcgtaacgcc agcggacgta cgggtgctgc tcatgggcgg
     1261 gagcctagcg agcacggctt aacggccggt caaaacgccg ccctctcaaa acaccggttg
     1321 cccaaagctc ggcgccacgt cttcgcccgg cgtggccgag cggcggtggg ccaacgcttc
     1381 agccacatgg acacggcgga cgccatcggc gccttcaagg tcggcgatcg tgcgcgagag
     1441 gcgcaggacg cgggtccagc cccgggcgct gatcttgcca gcctcggcgg cgcgggccag
     1501 cagggatcgg ccggcctcgt cgatggcgac gatcttttcc aggaaggcgc cctcggcccg
     1561 gccgttgaga gcctcaaggc tctcatgcgc ctcagcccgg tcgatctgca actgcctggc
     1621 ccgcgccacc cgggcggcga actcggccga gccttcgctg ggcggcggca gtgccaggtc
     1681 agcggcggtg acggcgggca tgtccaccga caggtcgatg cggtccatca gcggccccga
     1741 cacccggccc tgatagtcgc gctggcaccg gggggccttg ccgcaatagc cgcgccccgc
     1801 gccgccatgg ccgcagcggc aggggttcat ggccgccacc agttgcacct tggccgggta
     1861 gcgcacatgg gcgttggcgc gggccaccat cacctcgccg gtttcgagcg gacctctaag
     1921 actatcaagg gcctggactc cgaattcggg cagttcatca aggaacaaga cgccattgtg
     1981 cgccagcgac acctcgcccg gcttggcctt gtggccgccg ccggtcagag ccgccatgct
     2041 ggccgagtgg tgcggcgagc gaaacggccg cgcgcgggtc agggcccctc gagcgatcag
     2101 tccggccacc gaatgcacca tcgaggtctg caacagctca gtagaggtca ggggcggcaa
     2161 caaacccggc agacgctggg ccagcatcga cttgcccgag ccaggcgggc cgacgaacaa
     2221 gaggttgtga ccgccagcgg cggcgatctc caaggcgcgc ttggcgtgtt cctggccctt
     2281 gacgtcgcgc aggtccttgg cgcgctcgcc ttcgatcacc gggcccggcg agggcgcgga
     2341 cagcacctgg ctgccccgga aatggttgat caacgccacc agtgagcgcg gcgccaggat
     2401 gcgcgtaccg ccggcccagg ccgcttccgg tccgctggcc tccgggcaga tcaggcccag
     2461 gcccatggcc ccggcggcca tggcggcggg cagggcgccg gcggcgggca cgatgcggcc
     2521 gtccagagac agctcaccca tggccgccca gccgtcgagg gcgtccagcg ggatcacgcc
     2581 gatcgccgcc atcaccgcca gggcgatggg cagatcaaag tgcgtgccct ccttgggcat
     2641 gtcggccggg gccagattag cgacgatgcg cttgccgggc agggacagcc ccaggcccgc
     2701 aaaggctcca cgcacccgct cgcggctctc ggccacggcc ttgtcggcca agcccacgat
     2761 gacgaaaatc tgctgaccgc tggtcagctg cacctcgaca tcgacccggc gcgcctcaac
     2821 gccttcgaac gcaaccgtaa cgaccctggc ggccatattc gccccgtgga ggacctgtag
     2881 ttatccacac acgaaccatg gcgccgagag cggagcaaga acgaaaaacg aacgtttcgg
     2941 gaaatcagcg ttccaagcta ctcgctttat caagcggcgc ggatcgcctc gatgcggtcc
     3001 caaagagccg ccgacgcgtc ggcgccgccg aaccgcttga gctgctgggc cccggtcggc
     3061 gaggtcacgt tgatctcggt cagatactcg ccgattacgt caatgcccac gaacagcagg
     3121 ccccggcgct tcagttcggg ggcgatgatg gcgcacagtt ccaggtcgcg ggcggtcagc
     3181 tcgacggcct cggcgcgtcc gccgacccgc aggttggagc gtacctgacc ggcggccggc
     3241 acgcggttga tcgcgcccac gggctcgcca tcgaccagca gcacgcgctt gtcgcccttg
     3301 ctgacggcgg gcacgaactt ctgggcgatc acctgctcgc ggccgatcat ggcgtgcagc
     3361 tccagcaagg cgtcaagatt gggatcgtcg gccagcagcc gcgccacgcc cgaaccgcca
     3421 ccgccgtaga gcggcttgag cacgatgtcg ccatgccggg cgcggaagtc gtagatcgcc
     3481 tcatagtccg aggtgatcag ggtcggcggc tgcacgccgg ggaaatcggt gacgaacagc
     3541 ttctcgggcg cgtttcgcac ttcggccggg ttgttgacca cgagcgtgtg tggatgaacc
     3601 ttttcgagaa nnnnnnnnnn ggggtgagcg acggaaagcg cagcaccagc agcttttcgg
     3661 gagagttgcg cacccagaac gggtcgttca cgaccagggt cttcggctgg atcatctcca
     3721 gaagatgggt ggtggtgatg taggccatgt cgaagggcgg atcctggcgc atcagcacca
     3781 cgtcgacatc gtccttcatg tcgagcagca aactttcgcc gagctcggca tgggcgccct
     3841 tgacgctgct cagcaccagc gactgggcgc gggcgaagac ccggccgtct tccatcgaca
     3901 gcttgtcggg cgtgtaaaac cacagtttgt ggccccgggt ctgggcctcc atcatcatct
     3961 ggaatgtggt gtcggtgtcg atgttgatcc cgtgcacggg gtccatctgg acggcgactc
     4021 tcagcgacat gacgactccg gattttcgtg tgcgcgtcgc gccaggggcg acgcgtgtct
     4081 agttaagcct gaatggcggg aatgcgaggc ctagttcagc ctcaaacgta cccgctcccg
     4141 cgccgcccga gccgcgatcg cagcgccacc gtccaggatc tgggcctggg tcagcgcctg
     4201 cagcgctccc gcaagagcgc cctgcccctc ccgcgcggcc gccacatcca gccaaggccg
     4261 gtggtccgcg ggattgagca acgcccgccg cagggccgaa cgctcggccc ttagccagtc
     4321 acgggcctgc tgggcgcgag cgaagatatt attctgcaga cggatcagca cggcgcgatc
     4381 cggaatcggg gccatcaatc gttccaactt gtcggcgaca tcgggcatca gacccgtgcg
     4441 cagagcgcgg cgcgagagct ccgacggtag caccacccgc ccctcgccga acgggtcaag
     4501 ggccagaggg ccctcatcgg tctcgatgcg cagcaggaag tggccgggga aatcgacgcc
     4561 gtgcagatcg acacccgtca tgcgggccgc gtgcagatag aagacgccca gagccacggg
     4621 aaggcccttg cggcgccggg cgatggcgat gacgtcagcg ttgtcgggat ggtcgtaggt
     4681 gatcaggtcg cccgtcagat gcaggtcgcc ggccagggtc tcggcgacgg cttcctcggg
     4741 cgactcggtc tgcatgcgca cggccaggcg ttcgaccgcc tgttgggcca gggcgatgac
     4801 cggacccgtg tcgcggtcag ggtcatcgtg aacggcgcag gccagggccg cctcgaacag
     4861 cggaaagccg tcgtcagggc cttgcccggc ccgggtcagg atcgcctcgg cctcttcgcg
     4921 ggtcattgcg cctccatcca ggcatcgcgc aggtggcgtg gaagccggcc
//
LOCUS       DAOMEO010000070         4965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2124_length_4965_cov_2.467006, whole genome shotgun sequence.
ACCESSION   DAOMEO010000070 DAOMEO010000000
VERSION     DAOMEO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_4965_cov_2.467006"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>809)
                     /locus_tag="PLH31_02555"
     CDS             complement(<1..>809)
                     /locus_tag="PLH31_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88157.1"
                     /translation="EVLMWTVAYDNPGGRGAKVKDGEPGWQSDGTYLTATGERLTDAT
                     AQAAYLREVQDWIVRPQMRGVAGVAGVDSIGGYEKQFAVQADPARMAAYGVSFTELGK
                     ALEAANIAVGANFLERGGEAYLVRADARVRNIDEIANATVASREGVSIRVRDVANVQV
                     GGGLRTGAASENGEEVVVGTVLMLTGENSRTVAGASAERLAEIIKSLPPGIKVQVVYD
                     RSKLVNATIKTVEKNLVEGALLVIVVLFLLLGDFRAALITALVIPLSMLMT"
     assembly_gap    810..819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<820..1173)
                     /locus_tag="PLH31_02560"
     CDS             complement(<820..1173)
                     /locus_tag="PLH31_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88158.1"
                     /translation="MIGRILDFSVRARWAIIGVVALIAALGVYNLVRLPIDAVPDITN
                     KQVQINTVVPALAPAEVEKLVTFPVETAMAGIPGLESTRSISRNGFSQVTIVFKENTD
                     LYFARQQVSERLTGVG"
     gene            complement(1170..>1403)
                     /locus_tag="PLH31_02565"
     CDS             complement(1170..>1403)
                     /locus_tag="PLH31_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88159.1"
                     /translation="HWKTGVQGHLDVEAYDVVGARLGSSSVVWRGSLGGGGHNQAARY
                     RADFPGLSASQVTRVVVRYSPVAHAAEPSEAAR"
     assembly_gap    1404..1416
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1624..2859)
                     /locus_tag="PLH31_02570"
     CDS             complement(1624..2859)
                     /locus_tag="PLH31_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR88160.1"
                     /translation="MPRPTDKKTLLAAVAISSLLAAGVGAVIGRTMLAPKAETPTASA
                     AAAAEEGEHKEGEADHKEGEAEAGHEEEEGHIEMNAERLATSGIKLETLAVGGLGAGI
                     VAQASVIASPDGAATLAARADGAVVRINKRLGDPVRAGEALASIESRDAAAISAERAA
                     AAAKATASRQAYAREKRLFDAKITARQDLEAAQAEVAIAEAELRRASSAGLAAGVSAN
                     GRFVTVSSPISGRITAAPAVLGSYVTAGTELFRIADPRKIEVQASLPASDAGRVASGD
                     RAMIETAQGTIPAVVRSITPGVDVESRAATAILTVAQGQAGLVPGQALRVRITPRGAA
                     SGDRFSVPEESVQSVEGADIVFIRGKDGFTARPVKIGRRGGGRVEILSGVTAGEQIAG
                     QGAFLLKAELGKGEAEHGH"
     gene            complement(2869..4098)
                     /locus_tag="PLH31_02575"
     CDS             complement(2869..4098)
                     /locus_tag="PLH31_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR88161.1"
                     /translation="MPSPYARPAAVFGAVLALVSLGAPLRAAAAPVTLAAALERAQAQ
                     SPLITSAQAALAAAQGRARQAGFSPNPEASLESENIAGSGPYEGFSNAETTFSIGQRL
                     ELGGKRRTRAAAAQAEVEAAIVRVAIARADLIQEVKTQYAQALEADARVALAKEAAER
                     ANDLAGVAATLVEAGREPPLRALRARTASDEADAAVLAAQAQAAAARRALTSLWADPD
                     PEIDLVEPPAGPTLSAAIDPTASLDVRLAQAEREAAQAAIDRERAAGKPDLTIQAGVR
                     RFEQTGDQALIVGFTAPIPIRDRNQGNVAAARADATAAEARERLVLARSVRAVRDAQA
                     SLRAAEARLEVLQSRTVPQAQQAVDLARQGFQAGKFSLLDVLDAQAALSASRNDLIAA
                     RLERAKALAALERAAAQ"
     gene            complement(4156..4515)
                     /locus_tag="PLH31_02580"
     CDS             complement(4156..4515)
                     /locus_tag="PLH31_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88162.1"
                     /translation="MIKLTTWLKRTGGVVIACLLLSLVVAPAVDSAICGVELSSAESL
                     VVVESTASLDTAGDEHGKGDVDACLHGHCHHATPLIGAQTTQTDALAIAHARLTPAIA
                     GLPPSRASDRLSKPPRA"
BASE COUNT          766 a         1664 c         1717 g          795 t
ORIGIN      
        1 ggtcatcagc atcgacagcg ggatcaccag ggcggtgatc agcgccgccc ggaaatcacc
       61 cagcagcagg aagagcacca cgatgaccag cagcgcgcct tcgaccaagt tcttctcgac
      121 agtcttgatg gtggcgttga ccagcttgga gcggtcatag accacctgca ccttgatacc
      181 gggcggcagg ctcttgatga tctcggccaa tcgctcggcc gaggcgccgg cgacggtgcg
      241 gctgttctcg ccggtcagca tcaggacggt gccgaccacg acctcctcgc cgttctccga
      301 agccgcgccg gtccgaagtc cgccgccgac ttggacattg gccacgtcgc gcacccggat
      361 cgagacgcct tcgcggctgg cgaccgtggc gttggcgatc tcgtcgatat tgcgcacccg
      421 cgcgtcggcg cgaaccagat aggcctcgcc gccccgctcc aagaaattgg cgccgacggc
      481 gatgttggcc gcctccaggg ccttgcccaa ctcggtgaac gacacgccat aggcggccat
      541 gcgggccgga tcggcctgga cggcgaactg cttttcgtag ccgccgatcg agtcgacgcc
      601 ggccacgccg gccacgccgc gcatctgcgg tcgcacgatc cagtcttgaa cctcgcgcag
      661 ataggccgcc tgggcggtgg cgtcggtcag acgctcgccg gtggcggtca gataggtccc
      721 atccgactgc cagccgggct cgccgtcctt gaccttggcg ccgcgaccgc cggggttgtc
      781 gtaagcaacg gtccacatca gcacctcgcn nnnnnnnnna cccacgccgg tcagccgttc
      841 ggagacctgc tggcgcgcga aatagaggtc ggtgttttcc ttgaagacga tggtgacctg
      901 gctaaagccg ttgcgcgaga tcgagcgcgt gctctccagg cccgggatgc cggccatcgc
      961 cgtttccacc gggaaggtga ctagcttttc gacctcggcc ggggccaggg ccgggacgac
     1021 ggtgttgatc tgtacctgct tgttggtgat gtcgggcacc gcgtcgatcg gcagccgcac
     1081 cagattgtag acacccaagg ccgcgatcag ggcgacgacg ccgatgatcg cccagcgagc
     1141 gcggacggag aaatcgagaa tcctgccgat catcgcgcgg cctccgaagg ctcggccgca
     1201 tgggcgactg gactgtagcg aacgacgacg cgcgttactt ggctggcgct aaggccgggg
     1261 aaatcggccc gatagcgggc cgcctggttg tgaccgcccc cgccgaggga accgcgccag
     1321 acgaccgaag atgagcccaa tcgagcgccg accacatcat aggcctcgac gtccaggtgg
     1381 ccttgcacgc cggtcttcca gtgnnnnnnn nnnnnncgac gcaccaagcc ggatacgcta
     1441 aggccatcgc cgtcgttccg agccgtcgcg gtgagcacct tcagatggcc ttggcttacc
     1501 agctcgaagg cgggttgagg cgttgtccga ccctgggcta acgctggcgt cgccagagcc
     1561 gccaagatgg acagtccagc gcaagcgctt gcggggcgtg cgaggtttcg cattgttgcg
     1621 cgcctagtgc ccatgctcag cctccccctt gccaagctcg gccttcaaca ggaacgcgcc
     1681 ctggccagcg atctgctctc cggcggtcac gcccgagagg atttccaccc ggccgccacc
     1741 gcgccggccg atcttcaccg gccgcgccgt gaacccgtcc ttgccgcgaa taaagacgat
     1801 gtcggcgcct tcgaccgact gcaccgactc ctcgggcaca gagaaccgat cacccgaagc
     1861 cgccccgcgc ggcgtgatcc gcacccgcag ggcctggccc gggacgaggc cggcctgacc
     1921 ttgcgcaacg gtgaggatcg ccgtggcggc ccggctttcg acgtcgacgc cgggggtgat
     1981 cgaccggacc acggccggga tcgtgccttg ggctgtttcg atcatggcgc gatcgcctga
     2041 agcgacccgg ccagcgtccg acgctgggag cgaggcctga acctcgatct tgcgcggatc
     2101 ggcgatgcga aagagttcgg ttccggccgt cacataggat ccgagcacgg ccggggcagc
     2161 ggtgatgcgg ccggagatcg gactggagac ggtcacgaag cggccgttgg cgctgacgcc
     2221 ggcggcaagg ccggcgctgg aggcgcgccg cagctctgcc tcggcgatgg cgacctcggc
     2281 ctgggccgct tcaagatcct ggcgggcggt gatcttggcg tcgaacaggc gcttctcgcg
     2341 cgcataggcc tggcgagagg cagtcgcctt ggcggcggcg gcggcgcgtt ctgcgctaat
     2401 ggctgccgcg tcgcggctct cgatcgaggc caaggcttct ccggcgcgga ccgggtcgcc
     2461 gagacgcttg ttgatccgca ccacggcgcc gtcggcgcgg gccgccaggg tcgcggcgcc
     2521 gtcgggcgaa gcgatcaccg aggcctgcgc cacgatcccc gctcccagcc cgccgacggc
     2581 gagagtctcc agcttgatgc ccgacgtggc cagccgctcg gcattcatct caatgtggcc
     2641 ttcttcttcc tcatgtccgg cctcggcttc accctccttg tggtcggctt cgccttcctt
     2701 gtgctcgcct tcctcggccg cggctgcggc cgaggccgtg ggggtctcgg ccttgggggc
     2761 cagcattgtc cggccgatca cggcgccgac gccggcggcc agcaggctgc tgatcgccac
     2821 ggcggccagc agggttttct tgtcagtggg tctgggcatg tgggggtcct attgcgcggc
     2881 ggcgcgttcg agcgcggcca gcgccttggc gcgctcaagg cgcgcggcga tgaggtcatt
     2941 gcgggaagcc gacaacgcgg cctgggcgtc caggacatcg agcagcgaga acttgccggc
     3001 ctggaagccc tgacgggcta ggtccacggc ctgctgcgcc tggggcacgg ttcggctttg
     3061 cagcacctcc aggcgagcct cggcggcccg cagggaggct tgggcgtcgc gaaccgcgcg
     3121 gacggaccgg gccagaacca ggcgctcgcg cgcttcggca gccgtggcgt ctgcgcgggc
     3181 ggcggcgacg ttgccctggt tgcggtcccg gatcgggatg ggcgcggtaa agccgacgat
     3241 cagggcttga tcgcctgtct gttcgaagcg ccggacaccc gcctggatcg tcaggtcggg
     3301 cttgcccgcc gcgcgctcgc ggtcgatcgc cgcttgggct gcctcccgct cggcctgggc
     3361 cagccggacg tcgagggaag ccgtcgggtc gatcgcagcc gacaaggtcg gcccggctgg
     3421 cggttcgacc agatcgatct cagggtcggg atcggcccag agactggtca aagcgcgacg
     3481 ggcggcggcg gcctgggcct gggccgcgag caccgcagcg tcggcttcgt cgctggcggt
     3541 gcgggcgcgc agagcgcgta gcggcggctc acgacccgcc tcgaccagtg tcgctgcgac
     3601 gcccgccaga tcgttggcgc gttcggcggc ttccttggcc aaggcgacgc gggcgtcggc
     3661 ctcgagcgcc tgcgcatatt gcgtcttcac ctcttggatg aggtcggccc gagcgatggc
     3721 cacgcgcacg atggcggctt cgacctcggc ctgggccgcg gcggctcggg ttcggcgctt
     3781 gccgcccaac tcaaggcgct ggccgatcga gaacgttgtc tcggcgttgg aaaacccctc
     3841 atagggcccg gagccggcga tgttctcgct ctccaggcta gcttccggat tgggcgagaa
     3901 gccggcctga cgcgcccgac cttgggcagc ggcgagcgcg gcctgggcag aggtgatcaa
     3961 gggtgactgg gcctgggctc tttcgagcgc ggcggccagg gtgacgggag cggcggccgc
     4021 gcgcaaaggc gcgccgaggc tcacgagggc caggaccgcg ccgaaaacgg cggcgggccg
     4081 ggcatatggc gatggcatgt aatgtcccta cttacgttct gacgaaggcg aacacgcgcg
     4141 tcggcgcggt cggggtcagg cgcgaggggg cttactaaga cggtcggagg cccgtgaggg
     4201 cggcaggccg gcgatggccg gggtaagacg tgcgtgggcg atggcaagcg cgtctgtttg
     4261 cgtggtctgc gcgccaatca gcggcgtagc atgatgacag tgcccatgca ggcacgcatc
     4321 gacgtcgcct ttgccatgct catcgcccgc cgtgtcgagc gatgcggtgg attccaccac
     4381 caccaggctt tcggcagaag acagctcgac gccacagatc gccgaatcga ccgcaggcgc
     4441 aacgaccagc gacagcagca ggcaggcaat cacaacgcct cccgttcgct tcagccatgt
     4501 cgtcagcttg atcataaagt ttcctatatc ggcgaaagtc ttagccggga atgcgttaca
     4561 tagtcacaaa cgcaacctta ttcttatcca gtctgatcgt cgcaaaatcg ggccagatcg
     4621 cttggcagag ccatgggctt ctttggggcg acctccgcgg cgcctgtgtt tcccaccggc
     4681 agccagccaa acagggatag cagaccgccc gcagccaggc ggaggatctg gcctctcact
     4741 tcgcggccat ccctgcgttt ccacccgatc ctgagcatca tccaatgggt ccaagtgtgc
     4801 gctccggccc aaggctgacc caggatgtgg gctcgctcaa ggcggccaaa ggcttcggcg
     4861 aggcgccccg ccgcgaaggc ggccttggca agatcagcct cttggacata ggccaaccag
     4921 cgttcctgcg agttaagcat cgtgatcccc tgcagcgggc gaacg
//
LOCUS       DAOMEO010000071         4947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2130_length_4947_cov_3.718724, whole genome shotgun sequence.
ACCESSION   DAOMEO010000071 DAOMEO010000000
VERSION     DAOMEO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_4947_cov_3.718724"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLH31_02585"
     CDS             complement(<1..199)
                     /locus_tag="PLH31_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR88163.1"
                     /translation="MSLAALQSQTLQDLLKVEAERFVAQHPRSIAMAGQAAQVWRGGV
                     PMHWMGDWACPSPIFAMQGVGA"
     gene            complement(196..1686)
                     /locus_tag="PLH31_02590"
     CDS             complement(196..1686)
                     /locus_tag="PLH31_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQR88164.1"
                     /translation="MTLPAAITDAVARLTLPGLAVIDGALVEAASGAVFHNVSPRDGQ
                     VLNAVASCGQVDVDRAVASARAAFEDGRWRDLGPRQKKAILFRLAELMERDAEELALL
                     ESLDVGKPIRDARAVDIPLAINTCRWYAEALDKVYGEVGTSPVDRLSYAVHEPLGVIG
                     AIVPWNFPLHMAMWKVAPALAMGNSVVLKPAEQSPLTALKLGELALEAGLPPGVLNVV
                     PGLGATAGEALALSMDVDMIAFTGSGPVGRKLMEYSARSNLKRVSLELGGKSPQIVFA
                     DCPDLDAAAQAAAWGVFYNQGEVCTAASRLLVEASIKTDFVARVAAVARDMVPGDPLD
                     PGTVFGAMVSERQMNTALDYIATADSQGARRVLGGQRVRRDSGGFYVEPTIFDRVEAD
                     STLAREEVFGPVLGVMGFETEAEAFHLANDTVYGLAAGLWTSDINRALGGARKLKAGL
                     VWVNGWDACDITMPFGGFKQSGFGRDRSLHALHKYADLKSVSVTLR"
     gene            complement(1751..2410)
                     /locus_tag="PLH31_02595"
     CDS             complement(1751..2410)
                     /locus_tag="PLH31_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR88165.1"
                     /translation="MPLADSTAIHDQVYEAIRQRLITGRIAPGVGVSLRSLAAELGVS
                     PMPVRDAVRRLVAERALAINPANKRLSVPSLTAGRLEQLSQARLWIEPELAARAAPLA
                     DEALIRALQGIDARLDTALAAGDVDGYMTANHAFHFAIYARSGADVLLSMAGGLWLQI
                     GPFMRVVFGRVGTGSLPADRHAEAIAALKARDAQGARRAIAADLFEGMDKMRVAVEAG
                     S"
     gene            2521..3840
                     /locus_tag="PLH31_02600"
     CDS             2521..3840
                     /locus_tag="PLH31_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR88166.1"
                     /translation="MPDFGANNLDAFWMPFTPNRRFKAHPRMLSSARDMHYKTPEGRE
                     ILDATSGLWCVNAGHDRPRIREAIQKQAAEMDYAPCFNMGHPLAFAFASQLAQITPQG
                     LDRIFFTNSGSESVDTALKIALAYHRARGKGAKTRLIGRERGYHGVGFGGISVGGIPK
                     NRMYFGSLLTGVDHLPHTHGVAGNQFAKGEPEHGADLADTLEKIVALHDASNIAAVIV
                     EPVAGSTGVLIPPKGYLQRLRTICDRHDILLIFDEVITGFGRLGAPFAADRFGVTPDL
                     ICMAKGLTNAAVPCGAVAASGKIYDAMMDGADAPIELFHGYTYSAHPLACAAGIATLE
                     TYKEDDLFARAAGLEAYWQEAIHSLKDARHVIDIRNLGLIGGIELEPRAGAPTARAME
                     VFEAAFDAGLLIRVTADIVALSPPLILEKAHVDQVVETLRRVLATVD"
     gene            complement(4428..4613)
                     /locus_tag="PLH31_02605"
     CDS             complement(4428..4613)
                     /locus_tag="PLH31_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88167.1"
                     /translation="MASSSLQSAPFDARRAKRRTRQWPLGLSVLVMTVANAALWYGIY
                     ALTHGIFALDPSFFAGL"
     gene            4760..>4947
                     /locus_tag="PLH31_02610"
     CDS             4760..>4947
                     /locus_tag="PLH31_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR88168.1"
                     /translation="MPAPNALIALEQIGKIYDGAAVLTDASLSIAKGQFVALVGGSGA
                     GKTTLLKTINALIIPTTGR"
BASE COUNT          850 a         1769 c         1608 g          720 t
ORIGIN      
        1 gcgccccgac gccctgcatc gcgaagatcg gcgaggggca agcccagtcg cccatccagt
       61 gcatcggcac gccgccacgc cacacctgcg ccgcctgccc cgccatcgcg atgctgcgcg
      121 gatgctgggc gacgaagcgc tcggcttcga ccttcagcag gtcctgcagg gtctgtgact
      181 ggagggcggc gaggctcatc tcagggtcac cgacaccgac ttcagatcag cgtatttgtg
      241 cagggcgtgc aggctccggt cgcggccaaa ccctgattgc ttgaagccgc cgaacggcat
      301 ggtgatgtcg caggcgtccc agccattgac ccagaccagc ccggccttca gcttgcgcgc
      361 cccgcccagg gcccggttga tgtcggacgt ccacaggccg gcggccagac catagacggt
      421 gtcgttggca agatggaacg cctccgcctc ggtctcgaag cccatcaccc ccaggacggg
      481 accaaacact tcctcgcggg ccagggtcga gtcggcctcg acacggtcga agatggtcgg
      541 ctcgacatag aagccgccgc tgtccctgcg caccctctgg ccgcccagca cccgccgggc
      601 gccctggctg tcggcggtgg cgatatagtc cagggcggtg ttcatctgcc gctcgctgac
      661 catggccccg aacaccgtcc cgggatccag cggatcgccg gggaccatgt cacgcgccac
      721 ggctgcgacg cgcgccacga aatcggtctt gatcgaggcc tcaaccagca gccgagaggc
      781 ggccgtgcag acttcgccct ggttatagaa cacgccccag gccgcggcct gggcggcggc
      841 gtccaggtcg ggacagtcgg cgaaaacgat ctggggcgac ttgccgccca gctccagcga
      901 cacccgcttg aggttcgagc gcgccgaata ttccatcagt ttgcggccca ccgggcccga
      961 gccggtgaag gcgatcatgt cgacatccat cgacagggcc aaggcctcgc cagccgtggc
     1021 gcccaggccg gggaccacgt tcagcacgcc cggcggcagg cccgcctcga gcgccagttc
     1081 gcccagcttc agcgccgtga gaggcgattg ctcagcgggt ttcaggacca ccgaattgcc
     1141 catggccagg gcgggcgcga ccttccacat cgccatgtgc agcgggaagt tccacggcac
     1201 gatcgcgccg atcacgccca gcggctcgtg caccgcatag ctcagccggt cgaccggcga
     1261 ggtcccgacc tcgccataga ccttgtccag ggcttcggcg taccagcggc aggtgttgat
     1321 cgccagcggg atgtcgaccg cccgcgcgtc gcggatgggc ttgccgacat ccagactctc
     1381 cagcagggcc agctcttcgg cgtcccgctc catcagctcg gcgaggcgga acaggatcgc
     1441 cttcttctgg cgcggaccaa ggtcccgcca gcggccgtcc tcgaaggcgg cccgggcgct
     1501 ggcgacggcg cggtccacat cgacctgacc gcaggacgcc accgcattga gcacctgccc
     1561 gtcacgcggc gagacattgt ggaagaccgc gcccgacgcc gcctcgacca gcgcgccatc
     1621 gatcaccgcc agccccggca gggtcaggcg agcaacggcg tcggtgatgg cggcgggcaa
     1681 ggtcacggtc gcatattcct gaaactgtga tcacagttct cggaccagac gggactgtca
     1741 agccaggccg tcacgaccca gcctccacgg ccacccgcat cttgtccatg ccttcgaaaa
     1801 gatcagcggc gatggcgcgg cgggcgccct gcgcgtcgcg ggccttcagg gcggcgatgg
     1861 cctcggcgtg acggtcggcc ggcaggctgc cggtgcccac ccgcccgaac accacccgca
     1921 tgaacggccc gatctgcagc cacaacccgc cggccatcga cagcagcaca tcggcccctg
     1981 agcgggcgta gatcgcgaaa tgaaaggcgt ggttggcggt catgtagcca tcgacgtcgc
     2041 cggccgcgag cgcggtgtcg agccgcgcgt cgattccctg cagcgccctg atcagcgcct
     2101 cgtcggcaag gggcgcggcg cgggcggcca gttcgggctc gatccacagc cgggcctggg
     2161 acagctgctc cagacgcccg gcggtcaggg agggcaccga gagccgcttg ttggccggat
     2221 tgatcgccag ggcccgttcg gccaccaggc gccgcaccgc gtcgcgcacc ggcatcggac
     2281 tgacgcccag ctcagccgcg agactgcgca gggacacccc caccccgggg gcgatccgac
     2341 ccgtgatcag gcgctggcgg atggcctcat agacctggtc atggatggcg gtggaatcag
     2401 cgagcggcac tctttgcggg gcggcgggca cgggagggct tccagaaagc ggccaattgc
     2461 gatcacagtg catgatctgc cgcccaacac cagcccagcc gccagctctc gagacccgcc
     2521 atgcccgatt tcggcgccaa caacctcgac gccttctgga tgccgttcac gcccaaccgg
     2581 cggttcaagg cccatccgcg catgctctcg tccgccagag acatgcacta caagacgccg
     2641 gaaggccgcg agatcctcga cgccacctcg ggcctgtggt gcgtcaatgc cggccacgac
     2701 cgtccccgca tccgcgaggc gatccagaag caggcggccg agatggacta tgccccctgc
     2761 ttcaacatgg gccatcccct ggccttcgcc ttcgccagcc agctggccca gatcactccc
     2821 cagggcctgg accggatctt cttcaccaac tcaggctcgg agtcggtcga caccgccctg
     2881 aagatcgccc tggcctatca ccgcgcccgg ggcaagggcg ccaagacccg gctgatcggc
     2941 cgcgagcgcg gctatcacgg cgtcgggttc ggcggcatct cggtgggcgg catccccaag
     3001 aaccggatgt atttcggctc actgctgacc ggcgtcgacc acctgcccca cacccacggc
     3061 gtggccggca accagtttgc caagggcgag cccgagcacg gcgccgacct ggccgacact
     3121 ctggaaaaga tcgtcgccct gcacgacgct tccaacatcg ccgccgtgat cgtcgagccc
     3181 gtggccggct cgaccggcgt gctgatcccg cccaagggct atttgcaacg cctacggacg
     3241 atctgcgaca ggcacgacat cctgctgatc ttcgacgagg tgatcaccgg ttttggccgc
     3301 ctgggcgccc cgttcgccgc cgaccgcttc ggcgtcacgc ccgacctgat ctgcatggcc
     3361 aagggcctca ccaacgccgc cgtgccgtgc ggcgccgtgg ccgcctcggg caagatctac
     3421 gacgccatga tggacggcgc cgacgccccc atcgagctct tccacggcta cacctattcg
     3481 gcccacccac tggcctgcgc ggccggcatc gccaccctgg aaacctacaa ggaagacgac
     3541 ctgttcgccc gcgcggcggg cctggaggcc tactggcagg aggccatcca tagcctgaag
     3601 gacgcccgcc acgtcatcga tatccgcaac cttggcctga tcggcggcat cgagctggaa
     3661 ccccgcgccg gcgcccccac ggcgcgggcc atggaagtgt tcgaggccgc cttcgacgcg
     3721 ggcctgctga tccgcgtcac cgccgacatc gtcgccctgt cgccgccgct gatcctcgaa
     3781 aaagcccatg tggaccaggt ggtcgagacg ctgcggcgcg tgctggcgac ggtggattga
     3841 ggggaaaggg aggactgatc ccgctctatc cccggcgttc agcgaatcga agccagcaaa
     3901 atcaattact tctaactttc tcacacctct gtatccccgg ctccaaaacc tgagggatac
     3961 tgcttcctgt ccccgtcgca gggggagggc gcctggtacc ggcccaaagc ggcgacgggg
     4021 atggagctga gcggggccgc gacgccctgg aaggcgggtc gaggcgtgtg ggctgtctca
     4081 gaaggacgga ccaatcgctg agtggcttgt cggccgggtg aacgcgggtc gaagagggcg
     4141 agggatcgta aacccgccta tcgcgcattg tcggcgtcga gacggttagc cgattgacgg
     4201 aatgtgcgcc cgtacggggg ctcttgggta tcagagctga aagacgctcg cttggcccaa
     4261 tcctcacgca agaacagctt ctgcgaagcg gacaggtcga gccgaaacgc acaaaccaca
     4321 tcacggtcat ccgggttcgc cccggccgct tcgcgcctcc ccgacccctt cagtggctga
     4381 tgccgcgtga ggctgagaag ccgtgtccgg cgcaggccgg cgagtcccta aagccccgcg
     4441 aaaaagctcg gatccagggc gaagatgccg tgggtgaggg catagatgcc gtaccacagc
     4501 gccgcattgg ccacggtcat gaccagcaca gacaggccca gcggccactg gcgggtgcgg
     4561 cgcttggcgc ggcgggcgtc gaagggggcc gactgcaggg atgacgaggc catctgtgat
     4621 ctccgaatta accatcatca ggatcgagat tgagtcggtt ccccgccact gcgacattcg
     4681 ccgcgcgccg ccttgtcgca cccaggcttt ccccgcgcgg cgatccgggc cagggtggac
     4741 gaccgccgcc ggagaccgca tgcccgcccc aaacgccctc atcgccctgg agcagatcgg
     4801 caagatctat gatggcgcgg cggtgctgac ggacgccagt ctgagcatcg ccaagggcca
     4861 gttcgtggcc ctggtgggcg ggtcgggggc gggcaagacc accctgctga agacgatcaa
     4921 cgccctgatc atcccgacca ccggccg
//
LOCUS       DAOMEO010000072         4920 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2142_length_4920_cov_2.489825, whole genome shotgun sequence.
ACCESSION   DAOMEO010000072 DAOMEO010000000
VERSION     DAOMEO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_4920_cov_2.489825"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..371
                     /locus_tag="PLH31_02615"
     CDS             54..371
                     /locus_tag="PLH31_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HQR88169.1"
                     /translation="MTTFDDREQGFERKFVLDQDLEFKAAARRNRLLGEWAAGLMGLA
                     TVEEYARAVVKSDFEQPGDEDVLRKVFGDLKGSGVSVSEGEVRMKMDELLAQAREQLK
                     TGV"
     gene            554..1711
                     /locus_tag="PLH31_02620"
     CDS             554..1711
                     /locus_tag="PLH31_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQR88170.1"
                     /translation="MTTYHFETIGSADALAITAADTLIFATGPASAVGVRYSPLDLPL
                     PPRIDVTVGGRTVAFGSELSTLTQNGGAILPDGSRLFIGGSQNDQVTGTDASDGLYGG
                     AGADSLDGERGADFLQGNSGNDSLTGGFGANTVYGGQGDDLIIAGNGAETAGSFAHGN
                     LGHDEVIGGGGNDTLLGGQGDDFIAGRNGDDYLSGDLGEDELHGGEGADTLLGGAGKD
                     VIQTGGGADRVLGGDGDDLIVIELCGGAIVDGQAGNDTIVAAGLGKDILSGGEGSDRF
                     EFVTKTGPTEGQDDVILDWEAGDQFNFPQVSIYSILPMSYSEFAADSYAQALAIANQH
                     ISGPGAAYVAAQVGADVIVFADTNGDRSDGADIAVVLAGTSLNEIGLENFV"
     gene            1807..2055
                     /locus_tag="PLH31_02625"
     CDS             1807..2055
                     /locus_tag="PLH31_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQR88171.1"
                     /translation="MTVVTIHYAKTNLSRLLAEVEAGGEVVIARGKTPVAKLVPTPAE
                     PVKPKRYAGMWKGRIGFDESFFDPLPEDELALWNGEGD"
     gene            2052..2438
                     /locus_tag="PLH31_02630"
     CDS             2052..2438
                     /locus_tag="PLH31_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR88172.1"
                     /translation="MRLLLDTHVLIWWGEKNERLSRGAAQAIDSGDHEVLVSAVSAME
                     IAMKFRLGKLPSAKPLALAFEAETAAAGFLPLSISLTHARVAGSLPIAHKDPFDRLLI
                     AQALVEGITLVSNETVFDGAGISRLW"
     gene            complement(2422..>2773)
                     /locus_tag="PLH31_02635"
     CDS             complement(2422..>2773)
                     /locus_tag="PLH31_02635"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQR88173.1"
                     /translation="VALPYADRLTTLRQRGIGLWDVVEQAERRGSLDAAILNPRTNDL
                     LALVETLPALRAVAFNGGTSAKLGSRLLAPVADRIEFVALPSSSPAHASRSFAEKATA
                     WAVMKNWLLPTRGG"
     assembly_gap    2774..2800
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2801..2954)
                     /locus_tag="PLH31_02640"
     CDS             complement(<2801..2954)
                     /locus_tag="PLH31_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQR88174.1"
                     /translation="MAAERKRSFPPVVDPHTRVLILGSLPGEASLAAIQYYAHPRNAF
                     WRLLETV"
     gene            complement(2944..3684)
                     /locus_tag="PLH31_02645"
     CDS             complement(2944..3684)
                     /locus_tag="PLH31_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HQR88175.1"
                     /translation="MSDAPVLRLNPALDPAAFAQAYARDGLVKIPDVFEPAVVDRLAG
                     ILEQTLDWDVICSNEAGGAEILDRARRAQLGPDAVAARLHAATLRARTGFAYVHLGYR
                     MTDALVEGRDPGHPVHALTGFLNSPAFIAFGAAVTGETGLTGVDAQATLHRPADFQTL
                     RDGADPGHRPAGYTLGLTRQWRPDWGGQLLFHDKAGDVEHGFAPAFNGLTLFKAPRRH
                     SVAPVAPYAAAPRLAVTGWLRKDPPHGR"
     gene            complement(3687..4653)
                     /gene="purB"
                     /locus_tag="PLH31_02650"
                     /pseudo
     CDS             complement(3687..4653)
                     /gene="purB"
                     /locus_tag="PLH31_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517945.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
     assembly_gap    4654..4663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4664..>4920)
                     /locus_tag="PLH31_02655"
     CDS             complement(<4664..>4920)
                     /locus_tag="PLH31_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQR88176.1"
                     /translation="TKYKIWFEIEAHAADAMAELGVIPKLSAQTIWEKGRDAVWDSDR
                     IDEIERVTKHDVIAFLTHVSEIVGPEARFLHQGMTSSDVLDT"
BASE COUNT          824 a         1495 c         1718 g          846 t
ORIGIN      
        1 cgggccagcc cgtcacggga tatgcagccg acttacatgc tattggggcc ttcatgacca
       61 ccttcgacga tcgcgaacag ggtttcgagc gcaagtttgt tctggaccag gaccttgagt
      121 tcaaggcggc cgcgcgccgc aaccgcctgc tgggcgaatg ggctgcaggc ctgatgggtc
      181 tggccacggt ggaggagtat gctcgcgccg tcgtgaagtc cgattttgag cagccgggcg
      241 acgaagacgt tctgcgcaag gtctttggcg acctcaaggg ttcgggcgtc tcggtgtccg
      301 agggcgaggt tcgcatgaag atggacgaac tgctggccca ggctcgcgag cagctcaaga
      361 ccggcgtcta accgcgtcag gccagggctg aacgaacaag ggccgccctt tcggggcggc
      421 ccttttcttt tggccctcac ccggggtgga aacattgcat tttatttagt tgcgttttaa
      481 cgcaacagga tcgacgaacg tcgttatggc tgagctttgg cctagaggga ccctccacta
      541 gcgagattat tccgtgacga cctatcactt cgagaccatc ggatccgccg acgccctggc
      601 cattaccgcc gccgacaccc tgatcttcgc gacgggcccg gccagcgcag tcggagtgcg
      661 ctacagcccg ctggaccttc ccctgccgcc ccggatcgat gtgacagtgg gcggtcgcac
      721 ggtcgccttc ggctcggagc tttcgaccct gactcagaac ggcggcgcga tcttgcctga
      781 cggctccagg ctgttcattg gcggatccca aaacgatcag gtcaccggca cggacgccag
      841 tgacgggctc tatggcggcg ccggcgccga cagcctcgac ggcgagcgtg gcgctgactt
      901 cctgcagggc aacagcggca atgacagcct gaccggcggg tttggggcca ataccgtcta
      961 tggcggccag ggcgacgatc tgatcatcgc cggcaatggc gccgagaccg ccggctcctt
     1021 cgcgcacggc aatctgggtc atgatgaggt gatcggcggg ggcggaaacg acaccctgct
     1081 cggcggccag ggcgacgact tcatcgcggg ccgcaacggc gacgactatc tcagcggcga
     1141 cctgggcgaa gacgaactgc acggcggcga gggcgccgac acgctgctgg gcggcgccgg
     1201 aaaggacgtc atccagaccg gcggcggcgc ggatcgggtg ctgggcggcg acggtgacga
     1261 cctgatcgtc atcgagctgt gcggcggcgc gatcgtcgac ggccaggccg ggaacgacac
     1321 catcgttgcg gcgggcctcg gcaaggacat cctgtcgggc ggcgagggca gcgaccggtt
     1381 cgagttcgtg accaagaccg gcccgaccga ggggcaggat gacgtgatcc tcgactggga
     1441 ggccggcgac cagttcaact tcccgcaggt cagcatctat tcgatcctgc cgatgtccta
     1501 ttcggaattc gcagccgaca gctatgctca ggccctggcc atcgccaacc agcacatttc
     1561 cggccctggc gcggcctatg tggcggccca ggtcggggcg gatgtcatcg tgttcgccga
     1621 taccaacggc gaccggtcgg acggcgccga tatcgcggtc gtgctggctg gaacgtcgct
     1681 gaacgagatc ggtctggaga acttcgtcta ggcggtcgtg cgaggcgggg atgctgatga
     1741 gcgtccccgt cgccggattg atttttgccg aaaattagct aatctactta gctaagcgag
     1801 gcgcccatga ccgtcgtcac catccactac gccaagacca atctctcacg cctgctggcc
     1861 gaggtcgaag ccggcggcga ggtcgtcatc gcgcgcggga agacgccggt ggccaagttg
     1921 gtcccgacac cggccgagcc agtgaagccg aagcggtatg ctggcatgtg gaaggggcgc
     1981 atcgggtttg acgagagttt ctttgatccg ctaccagagg atgagttggc gctctggaac
     2041 ggtgaaggtg attgaggctc ttactcgaca cacacgtctt gatttggtgg ggtgaaaaaa
     2101 acgagcgcct gagtcgtggc gccgctcaag ccatcgactc tggcgatcac gaagttctgg
     2161 tcagcgcggt ttccgctatg gagatcgcga tgaagtttcg gctgggcaag ctgcctagcg
     2221 cgaagccctt ggccttggcc tttgaggctg aaacggcggc ggcgggcttt ctgcctttgt
     2281 cgatcagcct gacgcacgcc cgtgtcgctg gttcacttcc catagcccat aaggatccgt
     2341 ttgatcgttt gctgatcgct caggcgctgg tcgaagggat caccttggtg tcgaacgaga
     2401 cagtcttcga cggcgcgggc atcagccgcc tctggtaggc aaaagccagt tcttcattac
     2461 agcccacgcc gtcgctttct ccgcaaagct ccgcgacgca tgagcggggc tggaggacgg
     2521 taaggcgacg aattcaatgc ggtccgccac gggcgccagc aggcgcgagc ccagcttggc
     2581 cgacgtgccg ccgttgaagg ccacggcgcg cagggcgggc aaggtctcga ccagggccag
     2641 caggtcattg gtgcggggat tgaggatcgc ggcgtccagg ctgccgcgcc gctcggcctg
     2701 ctccaccaca tcccacaggc cgatcccgcg ctgtcgcagc gtcgtcaggc gatcggcgta
     2761 gggcagggcg accnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaccgtctc cagcaatcgc
     2821 cagaaggcgt tgcgggggtg ggcgtaatac tggatcgcgg ccagcgaggc ctcaccgggc
     2881 aggctgccca ggatcaggac gcgggtgtgc gggtcgacga ccggcggaaa gctgcgtttg
     2941 cgctcagcgg ccatggggcg ggtctttccg cagccagccg gtgacggcca gacgcggggc
     3001 ggcggcatag ggggcgacgg gcgccaccga gtgtctgcgc ggcgccttga acagggtcag
     3061 cccattgaag gcgggcgcaa agccgtgttc cacgtctccg gccttatcat ggaacagcag
     3121 ctggccgccc caatcgggcc gccactggcg ggtcaggccc agggtgtagc cggctgggcg
     3181 atgaccaggg tcggcgccgt cgcgcagggt ctgaaagtcg gccgggcgat gcagggtggc
     3241 ctgcgcgtcg accccggtga ggcccgtctc gccggtgacg gccgcgccaa aggcgatgaa
     3301 cgccgggcta ttgagaaagc ccgtcagggc gtggaccgga tggccggggt cgcggccttc
     3361 gaccagggcg tcggtcatcc gatagcccag atggacatag gcaaagccgg tgcgggcgcg
     3421 aagggtggcg gcatggaggc gggcggccac ggcgtcgggc cccaactggg cgcggcgggc
     3481 gcgatccagg atctcggcgc cgccggcctc attggagcag atgacgtccc agtcgagggt
     3541 ctgctccaga atgccggcca ggcggtcgac gaccgccggc tcgaacacat cgggaatttt
     3601 caccaggccg tcgcgggcat aggcctgggc gaaggctgcg gggtccagcg ctggattgag
     3661 gcgaaggacc ggcgcgtcgc tcatgctcag ccctgctcgc cgaacacgcg ccggaagatc
     3721 acgtcgacat tcttggtgtg gtagccgtag tcgaacagct cttcgaggac gctgtcggac
     3781 agggccttgg agaccacggg gtcggccttg aggaagtcga tgaaccgacc ctcgccgcgc
     3841 cacaccttca tcgaattgcc ctgcacggcg gcgtaggcgt cttctctgga gacgccctct
     3901 tgcgtcaggg ccagcatgac ccgctgcgag aacaccaggc cccccagctt gtcgaggttc
     3961 ctggccatgt tctcgggata gatgttgaag cgctcgatca cgctggccat gcggcgcagt
     4021 gcaaaatcga ggtggacggt ggcgtcgggg ccaatgccgc gctcgaccga cgagtggctg
     4081 atgtcgcgct cgtgccagag ggcgacgttc tcaagcgccg gggtcacggc cgagcggacc
     4141 agccgggcca ggccggtcag gttctcggtc aggatcgggt tgcgcttgtg cggcatggcc
     4201 gacgagccct tctggcccac atcgaaaggc tcctcggctt cgaggacttc ggtgcgctgc
     4261 aggtggcgga tctcggtggc caggcgctcg atcgacgagg cgaccacgcc cagggcgcag
     4321 aaataggccg cgtggcggtc gcgcgggatg acctgggtcg agaccggctc gacggtcagg
     4381 cccatcatct tggcgacgtg ttcctcgacg cgcggatcga cattggcgaa ggtgccgacg
     4441 gcgccgctga tcgcgcaggt ggcgatctcg aacttcgcca tcgccaggcg ctccttggcg
     4501 cgctggaatt cggcgtagta gccggcgagc ttgaggccaa aggtgatggg ttctgcgtgg
     4561 atgccgtggc tgcgaccgac gcagacggtc atcttgtgcc agatactgct cgtggaccgt
     4621 ggcgagcgaa ttgagccgtt gttgatacga cgcnnnnnnn nnngtgtcca gaacgtcgct
     4681 gctggtcatg ccctggtgca ggaagcgggc ctcggggccg acgatttccg acacgtgggt
     4741 caggaaggcg atgacgtcat gcttggtgac gcgctcgatc tcgtcgatgc ggtcgctgtc
     4801 ccagaccgcg tcgcggccct tttcccatat cgtctgggcc gacagcttgg ggatgacccc
     4861 aagttcggcc atggcgtcgg cggcgtgggc ctcgatctcg aaccagatct tgtacttggt
//
LOCUS       DAOMEO010000073         4903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2153_length_4903_cov_2.335396, whole genome shotgun sequence.
ACCESSION   DAOMEO010000073 DAOMEO010000000
VERSION     DAOMEO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_4903_cov_2.335396"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /gene="dnaK"
                     /locus_tag="PLH31_02660"
     CDS             complement(<1..1816)
                     /gene="dnaK"
                     /locus_tag="PLH31_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQR88177.1"
                     /translation="MSKIIGIDLGTTNSCVAIMDGKTPKVIENAEGARTTPSVVAFLE
                     DGERLVGQPAKRQAVTNPTNTLFAIKRLIGRNFADPVVAKDKAMVPYDIVKGPTGDAW
                     VKAQGKDYSPQEVSAFILQKMKEAAETHLGETVTKAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKNDGKKIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTFLGGEDFDLRIVDYLADEFKKEQGVDLRKDKLALQRLREEAEKAKKELSSTAQYEV
                     NLPFISMNASGPLHLNIKLSRSKLEALVEDLIARTIGPCEQALKDAGLKKSDIDEVIL
                     VGGMSRMPKVQQAVQDFFGREPHKGVNPDEVVALGAAVQAGVLQGDVKDVLLLDVTPL
                     TLGIETLGGVFTPLIERNTTIPTKRSQTFSTADDNQSAVTIRAFQGERPMAADNKFLG
                     QFDLQGIPPAPRGVPQIEVTFDIDANGIVNVHAKDKATNKEHSIRIQANGGLSDADIE
                     RMVKEAEANKASDEKKKALVEAKNQGEATVHATEKAMAEHGDKVAGEDKVAIETGLTD
                     LKTALEGEDAELIKAKTDALRQASMKLGEAMYASQQDAG"
     gene            complement(2154..4775)
                     /locus_tag="PLH31_02665"
     CDS             complement(2154..4775)
                     /locus_tag="PLH31_02665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQR88178.1"
                     /translation="MLLFAATGAPTVALASQTPGATAQTVTTQLPRTVVPSHYDVSFT
                     PDAANLTFTGSVQITVEVLEPTSTIVLQAHELTFAKASIKGSGKGAGAATIAVDDKAQ
                     TASFTFAKPVARGRHVLSIDYAGKINTQATGLFALDYETDAGKKRAIYTQFEAPDARR
                     FIPSWDEPFYKATFSLTATVPTGQMAISNMPAEKTVDVGGGKSRVTFATSPKMSTYLL
                     FFGLGEFERATTTAAGTQIGVITKKGDLAQAQFALKSAGPILEWYNDYFGVNYPLPKL
                     DHIAAPGQSQFFAAMENWGAIFYFEYALLLDPALSTQEDREGVYTTVAHETAHQWFGN
                     LVTMAWWDDLWLNEGFATWLEGRTTEKFFPEWNSALNAIAGREYAMGLDALATTHPVV
                     QPMKTVEEASEAFDGITYQKGQAVIGMLESYAGADAWRDGVRSYIKANTYGSTVTDDL
                     WGAVEVAAGKPIKAIARDFTLQPGVPLITVSSAGCVNGAAQLTLTQGEFTKDRPGKTP
                     LRWSVPVASRAVSGGAPVQTLVENGEAKVGVPGFGPVVVNAGQAGYFRTLYTPELFKG
                     LAANFASLQPIDQLGLLSDVYALGLAGLQPATDALDLSIATPIAADPKIWSRVTAIYG
                     AIDSYYDGMPAEQAQFRKVALASLSPLMTRVGWTAKSGESGPEATLRGDVIAVLGRLG
                     DPAVVAEAKRRYAADKTDPAAVPGPLRKTILGIVAASADAAGWEAMRAQAKAETNALL
                     RTELYTLLGAARDPALARRALELAMTDEPGATTSPNIISRVAGGHPDLAFDFAVANKA
                     AVEARVDASGRTGYLASLGGRSSDPAMLTKLDALTASDASLSREVEKAKAAVAYRLSV
                     REDQLPAITAWVKKTGG"
BASE COUNT          746 a         1553 c         1682 g          922 t
ORIGIN      
        1 cgcccgcgtc ctgctgcgag gcgtacatgg cttcgcccag cttcatcgag gcctgacgca
       61 gagcgtctgt cttggccttg atcagctcag cgtcctcgcc ttccagcgcg gtcttcaggt
      121 cggtcaggcc ggtctcgatg gcgaccttgt cctcgccagc aaccttgtcg ccatgctcgg
      181 ccatggcctt ttccgtggcg tgcacggtgg cctcgccctg gttcttggcc tcgaccagcg
      241 ccttcttctt ttcgtccgac gccttgttgg cctcggcttc cttgaccatg cgctcgatgt
      301 ccgcatcgga gaggccgcca ttggcctgga tgcggatcga gtgctccttg ttggtcgcct
      361 tgtccttggc gtgaacattg acgatgccgt tggcgtcgat gtcgaaggtg acctcgatct
      421 gcggcacgcc acgcggcgcc ggcggaatac cctgcaggtc gaactgaccc aggaacttgt
      481 tgtcggcggc catcggacgc tcgccctgga aggcgcggat cgtcacggcc gactggttgt
      541 cgtcagcggt cgagaaggtc tgcgagcgct tggtcgggat ggtggtgttg cgctcgatca
      601 gcggggtgaa cacgccgccc agggtttcga tgcccagggt gagcggggtc acgtccagca
      661 gcagcacgtc cttgacgtcg ccttgcagaa cgccagcctg aacggcggcg cccagggcga
      721 ccacttcgtc aggattgacg cccttgtgcg gctcgcggcc gaagaagtcc tgcaccgcct
      781 gttgcacctt gggcatgcgg ctcatgccgc cgaccaggat gacttcgtcg atgtcgctct
      841 tcttcaggcc ggcgtccttc agggcctgtt cgcacggacc gatggtgcgg gcgatcaggt
      901 cttcgaccag ggcttccagc ttggagcgcg acagcttgat gttcaggtgc agcgggcccg
      961 acgcattcat cgagatgaag ggcaggttca cttcgtactg agcggtgctg gacagttcct
     1021 tcttggcctt ttcggcctct tcgcgaaggc gctgcagagc cagcttgtcc ttgcgcaggt
     1081 cgacgccctg ctccttcttg aactcgtcgg ccagatagtc gacgatccga aggtcgaagt
     1141 cctcgccgcc caggaaggtg tcgccgttgg tcgacttcac ttcgaagacg ccgtcgccga
     1201 tttccagaac cgaaacgtcg aaggtgccgc cgcccaggtc atagacggcg atcttcttgc
     1261 cgtcgttctt gtccaggcca taggccagag cggccgcggt cggctcgttg atgatgcgca
     1321 gcacttccag gccggcgatc ttgccggcgt ccttggtggc ctggcgctgg gcgtcgttga
     1381 aataggccgg aacggtgatc acggccttgg tgacggtctc gcccagatgg gtctcggccg
     1441 cttccttcat cttctgcagg atgaaggcgg aaacttcctg cgggctgtag tccttgccct
     1501 gggccttgac ccaggcgtcg ccggtcggac ccttgacgat gtcataggga accatggcct
     1561 tgtccttggc cacgacgggg tcggcgaagt tgcggccgat caggcgcttg atcgcaaaca
     1621 gcgtgttggt cgggttggtg acggcctgac gcttggcggg ctggccaacc aggcgctcgc
     1681 catcctcaag gaacgcgacc accgacgggg tggtgcgggc gccctcggcg ttttcgatca
     1741 ccttgggggt cttgccgtcc atgatggcca cacacgaatt ggtggtgcca aggtcgatac
     1801 cgataatctt gctcatagtg tgaacctgtc gctcctgaat ggcggacggc ggtgacaagg
     1861 gccttagacg caagcgcccc ggtttttctc gctccgtccc cgtctggtcg aactttctaa
     1921 ccgttgatcc aatcctgccc gttgcgaccg aagtcgagcg ggcagagtgc gttccccgaa
     1981 gttgacgagc ccgtcaaccc cgcttggaag ccgatatggg ggatgcatac cgccccgcaa
     2041 gtccattccc tcatcttacg aaggggaatc ttcaagcccg atgatggaaa atggccgcgc
     2101 accccagccc tgggggcggc gcggccatcg tcaaggcata gacggtgtgg cgttcagccg
     2161 ccggtctttt tcacccaggc ggtgatagcc ggcagctggt cttcgcgcac ggagaggcga
     2221 taggccaccg ccgccttggc cttttcgacc tcgcgcgaca agcttgcgtc cgatgcggtc
     2281 agggcgtcca gcttggtcag catggccggg tctgacgagc ggccgcccag cgaggccagg
     2341 tagccggtgc ggcccgaggc gtcgaccctg gcctcgaccg ccgccttgtt ggcgaccgcg
     2401 aagtcaaagg ccaggtccgg atgcccgccg gcgacccggc tgatgatgtt gggcgaggtg
     2461 gtggcgcccg gctcgtcggt catggccagc tccagcgccc gcctggccag cgccggatcg
     2521 cgcgccgcgc ccagcagggt atagagctcg gtgcgcagca gggcgttggt ctcggccttg
     2581 gcctgggcgc gcatggcctc ccagccggcg gcgtccgccg aggcggcgac aatgcccagg
     2641 atggtcttgc gcagcgggcc tggtacggcc gccgggtcgg tcttgtcggc ggcgtagcgg
     2701 cgcttggcct cggccaccac cgccgggtcg cccagtcggc ccagcacggc gatcacgtca
     2761 ccgcgcaggg tggcttccgg accgctctcg ccagacttgg cggtccagcc gacccgggtc
     2821 atcagcggcg acaggctggc cagcgccacc ttgcggaact gggcctgctc ggccggcatg
     2881 ccgtcatagt agctgtcgat ggccccatag atggcggtca cccggctcca gatcttcgga
     2941 tcggccgcga tcggcgtggc gatggacagg tccagcgcgt cagtggcagg ctgcagaccc
     3001 gccaggccca gggcgtagac gtcgctgagc aggcccagct gatcgatggg ctgcaggctg
     3061 gcgaagttcg ccgccaggcc cttgaacagt tccggcgtgt agagggtgcg gaaatagccc
     3121 gcctggccgg cattgacgac cacggggccg aagcctggca cgccgacctt cgcctcgccg
     3181 ttctcgacca gggtctggac cggcgcgccg cccgacacgg cgcgcgaggc gaccggcaca
     3241 ctccagcgca gcggggtctt gccggggcga tccttggtga actcgccctg ggtcagggtc
     3301 agctgagcgg cgccgttgac gcagccggcg cttgagacgg taatcagcgg cacgccgggc
     3361 tgcagggtga agtcgcgggc gatggccttg atcggcttgc cggccgcgac ctcgaccgcg
     3421 ccccacaggt cgtcggtgac ggtgctgcca taggtgttgg ccttgatgta gctacgcacg
     3481 ccgtcgcgcc aggcgtcggc gccagcatag gattccagca tgccgatgac agcctggccc
     3541 ttctggtagg tgatgccgtc aaaggcctcg ctggcctcct cgacggtctt catcggctgg
     3601 accaccgggt gggtcgtggc cagggcgtcc aggcccatgg cgtattcacg gccggcgatg
     3661 gcgttgaggg cgctgttcca ctcggggaag aacttctcgg tggtgcggcc ttcaagccag
     3721 gttgcgaagc cctcgttcag ccaaaggtcg tcccaccagg ccatggtgac caggttgccg
     3781 aaccactggt gagccgtctc gtgggcgacc gtggtgtaga cgccttcgcg gtcctcctgg
     3841 gtcgaaaggg ccggatccag cagcagggca tattcgaaat agaagatcgc gccccagttc
     3901 tccatggcgg cgaagaactg gctctgaccc ggggcggcga tgtggtcgag cttcggcagc
     3961 ggatagttga cgccgaaata gtcgttgtac cactccagga tcgggccagc cgacttcaac
     4021 gcgaactgcg cctgggccag gtcgcccttt ttggtgatca cgccgatctg tgtcccggcc
     4081 gccgtcgtcg tggcgcgctc gaactcgccc aggccgaaga acagcaggta ggtcgacatc
     4141 ttgggcgagg tcgcgaaggt gacccggctc ttgccgccgc cgacatcgac cgtcttttcg
     4201 gccggcatgt tgctgatcgc catctggccg gtcggcacgg tggccgtcag gctgaaggtg
     4261 gccttgtaaa agggctcgtc ccacgagggg atgaaccggc gggcgtcggg agcctcgaac
     4321 tgggtgtaga tcgcgcgctt cttgccggcg tcggtctcat agtccagggc gaacagacca
     4381 gtggcctggg tgttgatctt gccggcatag tcgatggaca gcacatggcg gcccctggcg
     4441 acaggctttg cgaaggtgaa gctggcggtc tgggccttgt cgtccaccgc gatggtcgcc
     4501 gcgccggcgc ccttgcccga acccttgata gaggccttgg cgaaggtcag ctcatgggcc
     4561 tgcagcacga tggtgcttgt gggctccagc acctcgacgg tgatctgcac cgagcccgtg
     4621 aaggtcaggt tggcggcgtc gggcgtgaag cttacatcgt agtgcgaggg cacgacggtg
     4681 cgcggcagct gggtggtgac ggtctgggcg gtggcgcccg gggtctggct ggccagggcg
     4741 acagtcgggg cgccggtcgc ggcgaaaagg agcacggcga cagccgcgga agacagaaga
     4801 cgacgcatga acggccccaa gttggaaaat atggtcgacc aagatggtcg aaggcgcgaa
     4861 ccaacacctt ccgcccggtt ccggcgcgtt aaatatacgt aat
//
LOCUS       DAOMEO010000074         4841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2178_length_4841_cov_2.388425, whole genome shotgun sequence.
ACCESSION   DAOMEO010000074 DAOMEO010000000
VERSION     DAOMEO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_4841_cov_2.388425"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..424)
                     /locus_tag="PLH31_02670"
                     /pseudo
     CDS             complement(98..424)
                     /locus_tag="PLH31_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516778.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            830..3514
                     /gene="ppdK"
                     /locus_tag="PLH31_02675"
     CDS             830..3514
                     /gene="ppdK"
                     /locus_tag="PLH31_02675"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQR88179.1"
                     /translation="MPVETLTKSRWVYSFGGGGADGDASMKNLLGGKGANLAEMSSLG
                     LPVPPGFTVTTEACVHYYANGKQYPAELAQQVKDGLATIEKLTGKLFGDVANPLLVSV
                     RSGARASMPGMMDTVLNLGLNDETVEGLARLSGVRRFAYDSYRRFIQMYSNVVLDLEH
                     HMFEEILDDHKDRLDVHVDTGLTADDWAGVIKDYKAAVLEQLGRPFPQDAQEQLWGAV
                     GAVFASWMNDRAKFYRRMHDIPEAWGTAVNIQSMVFGNMGETSATGVAFTRNPSNGDN
                     RLYGEFLINAQGEDVVAGIRTPQSLTKIAREEMGDTVPSMEEAMPVVFAQFKSVVETL
                     ERHYRDMQDIEFTVEQGKLYMLQTRNGKRTAKAALKIAVDMAAEGVISRTEAINRVEP
                     ASLDQLLHPTIDPSAHRDVIATGLPASPGAATGKIVFDSDAAEKAAANGEAVILVREE
                     TSPEDIHGMHAARGIITARGGMTSHAAVVARGMGRACVSGAGEVGIFPKEGLFRVRGR
                     EFKAGEVITIDGSTGEILSGSPKMIEPELTGDFAMLMGWADEVRRLKVRANAETPLDA
                     KTARGFGAEGIGLCRTEHMFFDDTRIAAVREMILADDEAGRRTALAKIAPFQKADFVE
                     LFTIMEGLPVTIRLLDPPLHEFLPHTEEDVVAVAEATGLDAGKLMRRAKELHETNPML
                     GHRGCRLGVSYPEIYEMQVRAIIEAACDIAKSGKAAPIPEIMHPLVAKGEEMKYLRDL
                     TDRTAKAVLAEQGIAIEYTVGTMIELPRAALRAGDLAANAEFFSFGTNDLTQTTFGIS
                     RDDAGKFLGAYIDKGIFDKDPFVSLDQEGVGDLIRIAAERGRAARPDVKLGICGEHGG
                     DPASIAFCESVGLDYVSCSPYRVPIARLAAAQAALASK"
     gene            3698..4021
                     /locus_tag="PLH31_02680"
                     /pseudo
     CDS             3698..4021
                     /locus_tag="PLH31_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4124..4723)
                     /gene="wrbA"
                     /locus_tag="PLH31_02685"
     CDS             complement(4124..4723)
                     /gene="wrbA"
                     /locus_tag="PLH31_02685"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQR88180.1"
                     /translation="MAKVLVLYYSSYGHIETMAQAVAEGAREAGATVDIKRVPETAPL
                     EVAKAAHFKLDQAAPVATVAELADYDAVIIGTGTRFGRMSSQMAAFLDQAGGLWARGA
                     LNGKVGGAFTSTASQHGGQETTLFSIITNLLHFGMVIVGLDYGHADQMRIDEVTGGSP
                     YGATTIAGGDGSRQPTEGELAGARYQGRKVAETAIKLHG"
BASE COUNT          881 a         1567 c         1580 g          813 t
ORIGIN      
        1 gtcctgctcc gttcttgcgg cggacagatg ggtcccgcga acacgtcgcg ggatgacggt
       61 atttatagcg gtttcatggt ttatagcgcg ggcgccttca aacctgcctc ggaaccggcg
      121 tccagttcag cacggcgtcg taaaggtcat cccaacgcgg gttatcgcgt tcgagcaggt
      181 tgatcttcca ctgccgaggc cattccttca ttctccgttc ccgccggatc gcgaccgtca
      241 tgacctcgtg ggtttcgtaa cagaccaggc gcgtcaggcc ttagctcttg gtgaagccat
      301 ccctcaagcc ttcgcggtgc tggatcaacc gggcctcgaa ctgactgctg acgccgatat
      361 agagcgtgcc atagggctgg ttggtcatca tgtagacggc gatggatgcg tctctgggga
      421 ccattgcgcc aatatacact tatagataga tttcgtcatc ccgggacttg ttcccggggc
      481 ccatcgatcc gccgcgatgc ggagcaggat gattccgcgt cgtgaagctg aaccgtgggt
      541 cccgggaaca agtcccggga tgacgaagct caaatcaaaa tctatccgac tacggatccc
      601 cctccatccg cgaaatccca atcaagacaa cgttgtcatt ggaaatattt accgaatttc
      661 gtgacttcag cgcggaacgc aaagcgctgg tggacgcgtt cttgacctaa gtgctagtga
      721 acgccccgga tattgcagtg cgggatcacc gttcgacgct ttgtcccaag gcaagacgac
      781 cacccagagg gtcgcggcct gtttcactta tcgcagggtg aatttcatta tgccggttga
      841 gacgctgacc aagtcccgtt gggtctattc gttcggaggg ggcggcgccg atggcgacgc
      901 ctcgatgaag aatctgctgg ggggcaaggg cgccaacctg gccgagatgt cttcactggg
      961 cctgcctgtg ccgcccggct tcacggtcac caccgaggcc tgcgtccact attacgccaa
     1021 cggcaagcag tatccggccg agctggcgca gcaggtcaag gatggcctgg ccaccatcga
     1081 aaagctcacc ggcaagctgt ttggcgatgt cgccaacccg ctgctggtct cggtgcgctc
     1141 gggcgcccgg gcctcgatgc ccggcatgat ggacacagtg ctgaacctgg gcctcaacga
     1201 cgagaccgtc gaaggcctgg ccaggctgtc gggcgtccgg cgcttcgcct atgacagcta
     1261 ccgccgcttc atccagatgt attcgaacgt cgtgctcgac ctggagcacc acatgttcga
     1321 agagatcctc gacgaccaca aggatcgcct ggacgtccat gtcgacaccg gcctcacggc
     1381 cgatgactgg gccggggtga tcaaggacta caaggccgcc gtgctggaac agctgggccg
     1441 gcccttcccg caggacgccc aggagcagct gtggggcgct gtcggcgccg tgttcgccag
     1501 ctggatgaac gaccgcgcca agttctatcg ccgcatgcac gacatccccg aggcctgggg
     1561 caccgccgtc aacatccagt cgatggtgtt tggcaacatg ggcgagacct cggccacggg
     1621 cgtggcgttc acacgcaatc cgtccaatgg cgacaaccgc ctgtatggcg aattcctgat
     1681 caacgcccag ggcgaggatg tggtggccgg catccgcacg ccgcagtcgc tgaccaagat
     1741 cgcccgcgaa gagatgggcg ataccgtccc ctcgatggaa gaggcgatgc cggtggtgtt
     1801 cgcccagttc aagtccgtcg tcgagacgct ggagcgccac tatcgcgaca tgcaggacat
     1861 cgagttcacg gtagagcagg gcaagctcta catgctgcag acccgcaacg gcaaacgcac
     1921 cgccaaggcg gcgctgaaga tcgccgtcga catggcggcc gagggcgtga tctcgcgcac
     1981 cgaagccatc aaccgcgtcg agccggcctc gctggaccag ctgctgcacc cgaccatcga
     2041 cccgtcggcc catcgtgacg tgatcgccac gggcctgccg gcatcccctg gcgccgcgac
     2101 cggcaagatc gtcttcgaca gcgacgccgc cgaaaaggcc gccgccaacg gcgaggccgt
     2161 gatcctggtg cgcgaggaaa cctcgccgga agacatccac ggtatgcacg cggcgcgggg
     2221 tatcatcacc gcgcgcggcg gcatgaccag ccacgccgcc gtcgtggcgc gcggcatggg
     2281 ccgggcctgc gtctcgggcg ctggcgaagt gggcatcttc cccaaggagg gcctgttccg
     2341 ggtgcgcggt cgcgagttca aggccggcga agtgatcacc attgacggct cgaccggcga
     2401 gatcctcagc ggctcgccca agatgatcga gcccgagctg accggcgact tcgccatgct
     2461 gatgggctgg gccgacgagg ttcgccgcct gaaggtgcgc gctaacgccg agacgccgct
     2521 ggacgccaag accgcccgcg gtttcggcgc cgaaggcatc ggcctgtgcc gcaccgagca
     2581 catgttcttc gacgacaccc ggatcgccgc cgtgcgcgag atgatcctgg ctgacgacga
     2641 ggctggtcgc cgcacggccc tggccaagat cgcgcccttc cagaaggccg actttgtcga
     2701 gctgttcacg atcatggaag gcctgccggt caccatccgc ctgctcgacc cgccgctgca
     2761 cgagttcctg ccgcacaccg aggaagacgt cgtggcggtg gccgaggcga cgggcctgga
     2821 cgccggcaag ctgatgcgcc gggccaagga gctgcacgag accaacccca tgctgggcca
     2881 tcgcggctgc cgtctgggcg tctcctatcc cgagatctac gagatgcagg tccgggcgat
     2941 catcgaggcc gcctgcgaca tcgccaagtc gggcaaggcc gcgccgatcc ccgagatcat
     3001 gcacccgctg gtcgccaagg gcgaggagat gaagtacctg cgcgacctga ccgaccgcac
     3061 cgccaaggcg gttctagccg agcagggcat tgcgatcgaa tatacggtcg gcaccatgat
     3121 cgagcttccg cgcgccgccc tgcgggccgg cgacctggcc gccaatgccg agttcttcag
     3181 cttcggcacc aacgacctga cccagacgac gttcggcatc agccgcgacg acgccggcaa
     3241 gttcctgggc gcctatatcg acaagggcat cttcgacaag gatcccttcg tcagcctcga
     3301 ccaggaaggc gttggcgacc tgatccgcat cgccgccgag cgtggccgcg cggcgcgtcc
     3361 ggacgtcaag ctgggcatct gcggcgagca tggcggcgat cccgcctcga tcgccttctg
     3421 cgaaagcgtc ggtctcgact atgtgtcgtg ctcgccctat cgcgtgccga tcgcgcgtct
     3481 ggcggcggcc caggcggctc tggccagcaa gtaggtcaag gcttaagcgc aaaatcaggg
     3541 gcggtcgctg gtgaggcggc cgcccttttt gcattccaga acattgttat cgatttgccg
     3601 cgcgtcatgg ttaagctccc ggcgtccccg cgaagacggg gctggcaaag tgggagaaac
     3661 acctatgcgg accttgatga tgacggcggt tctggccctg gggatcagcg gcgcggccct
     3721 ggccgacccg atggccagcg cctatggcaa caccgttgcg atcacctatc ccaatggcgc
     3781 ggtggcgaag atgcacattg acgccgatgg cacctacacg accaaggcgc ccgacggcgc
     3841 gaccgcaaag ggggcgtgga agatcgaggg cggtaatacc tgcttcaccc agaccgaccc
     3901 ggccccgccg gcgggcatgg cggccaactg cagcccgacg gctgacaaga aggtcggtga
     3961 cagctggacc gccccgggtc ccaacggctc aaccctgacc gtcagcatcg tggcggggcg
     4021 ttaagccctc aagacgcccc atgaaaactg ccccgaagcc tgcgaggctt cggggcggct
     4081 cttcgccgtc ctaccggggg agggaacgcg gacggcggaa cgcttagccg tgcagcttga
     4141 tcgccgtctc ggcgaccttg cggccctggt agcgggcgcc ggccagttcg ccttcggtgg
     4201 gctgacgcga gccgtcgcca ccggcgatgg tggtggcgcc ataggggctg ccgccggtga
     4261 cttcatcgat ccgcatctgg tcggcgtgac catagtccag acccacgatc accatgccga
     4321 agtgcagcag gttggtgatg atcgaaaaca gggtggtttc ctggccgcca tgttgcgagg
     4381 cggtcgaggt gaaggcgccg ccaaccttgc cgttcaaggc gccgcgcgcc caaagaccgc
     4441 cggcctggtc gaggaaggcc gccatctgcg acgacatccg gccaaagcgc gtgcccgtgc
     4501 cgatgatcac cgcgtcatag tcggccagtt cggcgacggt ggcgacgggc gcggcctggt
     4561 cgagcttgaa gtgggcggct ttcgccacct ccagcggggc ggtctcagga acgcgcttga
     4621 tgtcgacggt tgcgccagct tcgcgggcgc cttcggcgac cgcttgagcc atggtctcga
     4681 tatgaccgta ggacgaataa taaagaacca gaaccttggc catgagggcc tccttgactg
     4741 tgggattggg gaaatggggc ggggcaagcg tgaaggctcg tctcgcccgg ggattgaagg
     4801 aaacggccgc gtttaggcgg cgtcgaccag gaccagctcg g
//
LOCUS       DAOMEO010000075         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2208_length_4766_cov_3.281893, whole genome shotgun sequence.
ACCESSION   DAOMEO010000075 DAOMEO010000000
VERSION     DAOMEO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2208_length_4766_cov_3.281893"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>245)
                     /locus_tag="PLH31_02690"
     CDS             complement(<1..>245)
                     /locus_tag="PLH31_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HQR88181.1"
                     /translation="DLNCRNQDRRRHYLCEPGWRADAAIQGLGRSHRTNQASAPLFCP
                     VTTDIHGEKRFTSTISRRLDSLGALTKGERRTAGNGL"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(256..3134)
                     /locus_tag="PLH31_02695"
                     /pseudo
     CDS             complement(256..3134)
                     /locus_tag="PLH31_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664960.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
     gene            complement(3352..4008)
                     /locus_tag="PLH31_02700"
     CDS             complement(3352..4008)
                     /locus_tag="PLH31_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88182.1"
                     /translation="MVAQLFKQLALHGGSGQEESALTINATGYRRGQTPAIGALDGDV
                     RGRLEARRVDYKASGLRPIAWVDGLAHGDKMALLAELTAITLNLREARTSNIRNSARA
                     EAIELAELCAADISAHWTPDPDYLAVHSKKQLLVLLDEMQVDDPRAKTLKKDELVVLV
                     ADAAAERQWAPRVLSWEGATVETPPADEEQDDGDEALADDLTPGPAAPSPEVAVQHAA
                     "
     assembly_gap    4090..4099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4100..>4766)
                     /locus_tag="PLH31_02705"
     CDS             complement(<4100..>4766)
                     /locus_tag="PLH31_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR88183.1"
                     /translation="MARYTAAGGRVESDLFAELADVLLDPGKLQDLWRERAAPFIEGF
                     KQLGLAVYIGRDAGFRAPEGFETLPYVYPGDLTDETKAVLAAARQRVAQAARDLGGID
                     LAADDAALTIFPLLQAKMEVASAPLKRLALGAVILSPDGATGISAEFFAAPVSEELLD
                     EAGDDEADEDDASGGQGNGARYGRSASDVEVPKADVDVEGSSHVLHETRTDVATRGLI
                     RDLA"
BASE COUNT          693 a         1673 c         1674 g          706 t
ORIGIN      
        1 aagaggccat tgccggccgt tcggcgctcg cccttggtca gggcgcccag gctgtccagc
       61 cgccgcgaga tcgtcgaggt gaaccgcttc tcgccgtgga tgtcggtcgt gaccggacag
      121 aacagcgggg cgctggcctg gttggtccgg tgcgaccggc caaggccctg gatcgccgcg
      181 tcggcccgcc agccgggctc gcagagatag tgccggcgac ggtcctggtt ccggcagttc
      241 aggtcnnnnn nnnnnttgct ggttcttcgc atccaggctg gcgtggtagc tgcgccccgt
      301 gccgccggcg tcggagaaca ccagcacccg cttcttgccg ctcatgaagg cgtcggtctc
      361 ggccttggcg ctcgacgcgc tgcggcgctc cacgacccgg cgtccatcgc gcaggacgac
      421 ccggcgcgag cggccggtga tctcggcgac gttgtcgggg ccgagcgcgc ccaggaccgc
      481 gtccagcaca cccggaacgg cgggcaggca ggccaggtgg acgatcagcg cctcccgcag
      541 acgcagggct tcctggctga cgaccggctg accatccacg atcaacggac gcatcgtcac
      601 attgccttcc tcatcctcga tcgcctccat ggcggcgata gggaaggccc ccatcagata
      661 gtcgagcacc tggtccttgg gggtcaggtc gatggtcagg ttgttccact cttcgggcgg
      721 gatctcggcc agccggcgct ccatcaccgc ctcattggtc gagacgatct gcacgacgct
      781 ggagcgtccc tggacgaggt cgtcgcggat cgccgcgatc agggtcggcg acttcaggcc
      841 cgccagcagc gcgccgaaga agcgcagctt ggcgccctcg aaagccgaca tgaccgccga
      901 agcggcctgg ccgctcttag ccttgccgtc ctcggtgatg ccgaccgcct ccagggcggc
      961 gcgcaggttc tggtggatca gctggtaggc gtcggcccag gcgttccaga tctcgacgtc
     1021 gtcgccggtc agcacatggc gcagcgcctg atattccacc ccgtcgaacg acagcgaccg
     1081 ggcgatgtag aggccgagcg ccttcagttc gcgggcgatc aactccatga ccgccacgcc
     1141 gcccttctcg acggcgtcca tgaaggcgtc gcgggtgttg aacggcgcct ccggcccgcc
     1201 ccacaggccc agacgagcgg cataggccag gttctcgggc gtggtcgcac ccgtggcgct
     1261 gacatacatc acccgcgcat tgggcaacct gttctgcagg gccaggcccg ccatcccctg
     1321 ttgggaggcc ttcttgggcc cacgagcgcc ctggccgccg cccgccgcgt tggccatggc
     1381 gtgggcttcg tcaaaggcga tcaccccgtc gaagtcggcg cccagccagc cgacgatctg
     1441 gtcgaggcgc gaaagacgct cgccccgcgc cggctgacgc agggtggcgt aggtggtgaa
     1501 caggaccccc cggtccatgc ggatcgcgtc ggcctgcttc caggcgctct gcggggtgat
     1561 gtcggaggcg gagccgccga tcgccgtcca gtcccgacgt gcgtcctcca gaagcgtgtc
     1621 gttcctggaa atccacaccg ctttcaggcg gccctgggcc atgttgtcgg cgatgacccc
     1681 ggcggcctgg cggcccttgc cgcagccggt gccgtcgccg aggaagaagc cgcggcgcag
     1741 ctggaaggcg ccttcaacgc cctccttgac cagcaccagc ttgtgcggcg cttcgcccag
     1801 catccaccag cccggcagaa gccccgactg cgcctcgccg gcgtagatca cggtctcgat
     1861 ctgggcgtcc gacagcatcc cctcctggac gatgttggtc ggcaggagcg ggcgataggt
     1921 cggggccggc ggcgccaccg acgccatggg gccggactcc accagcgcgg aggggtgtgg
     1981 acggctcccc tgcaaggcca ggcggctgac ccggtaggcc tggtagaggc cgacgtcgtg
     2041 gccctcgccc gaccaggcga tggtttcgaa cgccaccgac gacgccccgg ccaggaaccc
     2101 caggcgggtc gagggggtgg ccagggcgcg ggcgcgcggc gccaggaagg cgacgttgga
     2161 gaccgatcgg aactggcgcg gctgggcggt cgggcgcgga tccagcacgg cgatcagctt
     2221 ggcgatgtcg gccagggtct cgccgctgac gaccttgacg gcgccgacgc cgcactcttc
     2281 gaggcggtcc aagaccagca ggccggtctc gacggaggtc ccgtgcttgg cataggcgcg
     2341 gggcgggaag gcgatcaacc ccaccaccct gccgcggcgg gcgagcgcct gcatcgcccc
     2401 ggcgtcctcg aagaggcgcg tgggtacgat ggcgctgagc cgtccgccct cggcaaggcg
     2461 gcgtgcagat gaccctccag ccgctggaac ggcggattgg cgatcacggc ctggaagctg
     2521 cctgaagagg gcaggatgtc cttcaggtgc gccgcgtcat ggcgggtgcg cgaggcggcc
     2581 gggaacaggc catccagcag agccgaacgg tgcggcgcga tctcattgag ctccagcgtc
     2641 gccccgcacg cctcggcgac gatcgccaac aggccggtgc cggccgacgg ttcgagcacc
     2701 ttgtcgccag gccggacctg ggcggccagc accgccaagg cggccaaggc gggcggcgtg
     2761 gagaactggt cgagcgcgac ctgctcctcg ctgcggcggg tgtgggtcaa ggtcaggtcg
     2821 gtgacgctgg ccagcaaggc cgcgatctcg gccggagcgt cctcgagccg gccaatctgc
     2881 gggctgagcc gacgcagctg caggaccagg gccgcctcga tggcgtcgta ggcgtcgcgc
     2941 cacgaccagg cgccctcggc gtccgagccc ccgaaggaga tggtcatggt gctggccacc
     3001 agcttgcgat ccagcgcccg cgaccgattg aggtgcgggg tgagcgcccg ggcggcggcc
     3061 agcgtgttgg cggccttgtc ctgggcggcc cctggggtct gaagggcgct gaagaggggg
     3121 aatgtcgcgt tcatggcgtg ggtctcggat cacccgtggc ggagggctcc tccctccagt
     3181 cccatcgggc tcaccaagcc cgctcccttc ccccctcctt taggcgccaa gggcgagatc
     3241 accggatcca gccgcgtcga aggcggaaac gaaaagagga ccccgccggc gctgccggcg
     3301 aggtcctctg agagccaagg ccgcctggac gtatccagat gcgacccccg atcaggccgc
     3361 gtgctgaacc gcgacctccg gggaaggtgc ggccgggcct ggggtcaggt cgtcggccag
     3421 ggcttcatca ccgtcgtcct gctcctcatc ggccggcggc gtctcaaccg tggcgccctc
     3481 ccaggacagc acccgcggcg cccactggcg ctcggccgcg gcgtcagcca ccaggacgac
     3541 cagctcgtcc ttcttcaggg tcttggcccg aggatcgtcc acctgcatct cgtccagcag
     3601 caccagcagc tgcttcttgg agtggacggc caggtagtct gggtcgggcg tccagtgcgc
     3661 cgagatgtcg gcggcgcaga gttcggccag ctcgatggcc tcggcccgag ccgagttgcg
     3721 gatattgctg gtccgggctt cccgcaggtt cagggttatg gcggtgagtt ccgccagcaa
     3781 ggccatcttg tcgccgtgag cgaggccatc gacccaggcg atgggccgaa gccccgaagc
     3841 cttgtagtcc acccgccgcg cctccagccg gccccgaaca tcaccgtcca gcgcgccgat
     3901 cgccggcgtc tggccccggc ggtagccggt ggcgttgatg gtcagcgccg actcctcctg
     3961 gcccgacccg ccgtgcagcg ccagctgctt gaacagctgg gcgaccaagg ccgtcagcgc
     4021 agcggccgga ttgtcggccc cattttctct actggggttg gcttaggtga gtcaggctta
     4081 gtggcaattn nnnnnnnnng gcgagatcac ggatcagccc gcgcgtggcg acgtcggtgc
     4141 gggtctcgtg caggacgtgg ctggagccct cgacatcgac gtcggccttg ggcacctcca
     4201 cgtcgctggc ggagcgaccg tagcgcgcgc cgttgccctg gccgccgctc gcatcgtcct
     4261 cgtcggcctc gtcatcccca gcctcgtcca gcagctcctc ggacaccggc gcggcgaaga
     4321 actcggccga gatccccgtc gccccgtcgg gcgacaggat gacggcgccg agcgctagcc
     4381 gcttcagcgg agccgaggcc acctccatct tcgcctgcag cagggggaag atcgtcagcg
     4441 ccgcatcgtc ggcggcgaga tcgatgccgc cgagatcacg cgccgcctgg gccacccgct
     4501 gccgcgcagc cgccagcacc gccttggtct catcggtcaa gtcgccaggg tagacgtagg
     4561 gcagggtctc gaagccttcc ggagcccgga agccggcgtc tcgcccgatg tagacggcga
     4621 gcccaagctg cttgaacccc tcgatgaagg gggcggcgcg ctcccgccac agatcctgca
     4681 gcttgccagg atcgagcagg acatcggcca gttcggcgaa caggtcggac tcgacccgcc
     4741 cgccggcggc ggtgtagcgg gccatc
//
LOCUS       DAOMEO010000076         4766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2209_length_4766_cov_2.528550, whole genome shotgun sequence.
ACCESSION   DAOMEO010000076 DAOMEO010000000
VERSION     DAOMEO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_4766_cov_2.528550"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1842
                     /locus_tag="PLH31_02710"
     CDS             37..1842
                     /locus_tag="PLH31_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HQR88184.1"
                     /translation="MPGLEAMMSTEQRQMLEALSANLARAAVTAQGAIAEAALRQADR
                     PAALNADPFHVAPAMNEVMSRLAAQPERMLRAQADLFSRYMDLWQSAARRAAGEETKP
                     VVTPASGDKHFNDPDWASNPMFDMMKQSYLLTSNWLNGLVSEVDGVDPGSKRRVEFFT
                     KMLTDAFSPSNFLISNPAALREVMQTNGESLVRGMENFAADLDRGGGQLAISQTDLEK
                     FKVGENVATAPGKVVYQNDILQLLQFDPTTEQVHEIPLLIFPPWINKFYIMDLRPENS
                     MIRWLTGQGFTVFVTSWVNPDAKLAAKTFEDYMLEGVYDATQQVMTQTGVDKVNTVGY
                     CIGGTLLSCALAHMAAKGDKRINSTTFFAAQQDFSEAGDLLLFTNEEWLQSIEQQMDA
                     TGGFLPSQSMADTFNSLRGNDLIWSFFISNYLMGKEPRPFDLLFWNADQTRMPKSLHM
                     FYLRNFYKDNALTTGALTLGGEKLDLSKVKIPIYVQSSRDDHIAPYRSVYRGARAFGG
                     PVTFTMAGSGHIAGVINHPDAKKYQHWTNGALPGSVEDWISGAQEHPGSWWPHWAEWL
                     RARSGPLVPARDPAKGKLKPIEDAPGSYVRVRSDA"
     gene            1895..2527
                     /locus_tag="PLH31_02715"
     CDS             1895..2527
                     /locus_tag="PLH31_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HQR88185.1"
                     /translation="MLELGDMPAISRRTVLGMILAAPAFGAAAPARDGMGLARAARAQ
                     IGVTKAYDASYRAIAYPQGDVLRTTGVCADVLVRAARDAWKIDLQQRIHEDMVRDFDA
                     YPSRRTWGLKRPDANIDHRRVLNLETYLTRQGALLKRNAKRVAGDRFDDPLPGDILTW
                     RVWGERPHLGIVARGPEGVRVVHNIGFGTKEEPLWMFKLHGAVAHYRWRV"
     gene            2587..2841
                     /locus_tag="PLH31_02720"
     CDS             2587..2841
                     /locus_tag="PLH31_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQR88186.1"
                     /translation="MAAELSITAKGQITLKKSVLDHLGVSPGQKVDVRLLPNGRVELR
                     PTSSKRDLSSVFGILKREGQRTVSIEEMQDAIEAGACGEP"
     gene            2838..3224
                     /locus_tag="PLH31_02725"
     CDS             2838..3224
                     /locus_tag="PLH31_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR88187.1"
                     /translation="MRVALDTNVIVRFLVVDDEPQALAARALLHTADQILISTLVLCE
                     TVWVLSRAYRYPAASLADALRQLTDIQGVEIDRPIFEAGLAMLERGGDFADGCILFEA
                     GRAKCDRLATFDAGLIALSQGRASLP"
     gene            complement(3246..4193)
                     /gene="argC"
                     /locus_tag="PLH31_02730"
     CDS             complement(3246..4193)
                     /gene="argC"
                     /locus_tag="PLH31_02730"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQR88188.1"
                     /translation="MAPKVFIDGEVGTTGLQIRERLVGRTDLQLISIDPDKRKDPAAR
                     AAMLNGADAVILCLPDEASKEAVGLITNPDVKVIDASTAYRIDPGWAFGFPELDAEQR
                     GKIAASRRISNPGCYSTGAIALTRPLVSAGILPAELPLSFNAVSGYTGGGKAMIAEFE
                     DEAAPNYTRAPYRIYAMGLSHKHVPEIQKHGGLLTRPIFTPAVGRYAQGMLVELPLHL
                     AMLEDGRCLHDIHAAYAAHYKGEQFVEVASLDEAKGLTTLDPEGLNGTNRLKVFVFGS
                     DTSGQARLVALLDNLGKGASGAAVQNLNLVLGLEEAAGL"
     gene            complement(4282..4755)
                     /gene="rpsI"
                     /locus_tag="PLH31_02735"
     CDS             complement(4282..4755)
                     /gene="rpsI"
                     /locus_tag="PLH31_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQR88189.1"
                     /translation="MSEAQGFDALASLSSNPEAAAPAVQKIDAQGRAYATGKRKNAIA
                     RVWIKPGKGTITINGRDQEVYFARPVLRMMIAQPLQVTDRLGQFDVVVTVEGSGLSGQ
                     AGAVRHGLSKALTYYEPSLRPVLKPHGFLTRDSRVVERKKYGKAKARRSFQFSKR"
BASE COUNT          872 a         1566 c         1499 g          829 t
ORIGIN      
        1 gctacggccg atcgggtggc gccgataggg accaagatgc ccggtcttga ggccatgatg
       61 tcgacggaac agcgccagat gctggaagcc ctgtcggcca atctcgcgcg cgccgccgtc
      121 accgcccagg gcgccatcgc cgaagccgcc ttgcgccagg ctgatcgccc cgcggctctc
      181 aacgccgacc cgttccacgt ggccccggcc atgaacgagg tgatgtcccg cctcgccgcc
      241 cagcccgaac ggatgctgcg cgcccaggcc gacctcttca gccgctatat ggacctctgg
      301 cagtccgccg cccgccgcgc cgccggcgaa gagaccaagc cggtcgtgac gcccgccagc
      361 ggcgacaagc acttcaacga tccggactgg gcgtccaacc ccatgttcga catgatgaag
      421 cagtcctacc tgctgacctc gaactggctg aacggcctgg tgtccgaggt cgatggtgtg
      481 gatcccggct ccaaacgacg ggtcgagttc ttcaccaaga tgctgaccga cgccttctcg
      541 ccatcgaact ttctgatctc caaccccgcg gcgctgcgcg aagtgatgca gaccaacggc
      601 gagagcctgg tgcgcggcat ggaaaacttc gccgctgacc tggatcgcgg cggcggccag
      661 ctggcgatca gccagaccga tctggaaaag ttcaaggtcg gcgagaacgt cgccaccgcc
      721 ccgggcaagg tcgtctacca gaacgacata ctgcagctgc tgcagttcga tccgaccacc
      781 gagcaggtcc acgagattcc gctgctgatc ttcccgccgt ggatcaacaa gttctacatc
      841 atggacctgc ggcccgaaaa ctcgatgatc cgctggctga ccggccaggg cttcacggtg
      901 tttgtgacct cgtgggtcaa tcccgacgcc aagctcgccg ccaagacctt cgaggactac
      961 atgctggagg gcgtctatga cgccacacag caggtcatga cccagactgg cgtggataag
     1021 gtcaacacgg tcggctactg cataggcggc acccttctgt cctgcgccct ggcccacatg
     1081 gcggccaagg gcgacaagcg gatcaattca accaccttct tcgccgccca gcaggacttc
     1141 tccgaggccg gcgatcttct gctcttcacc aacgaggaat ggctgcagtc gatcgagcag
     1201 cagatggacg cgacgggcgg cttcctgccc agccagtcaa tggccgacac cttcaacagc
     1261 ctgcgcggca acgacctgat ctggtcgttc ttcatcagca actatctgat gggcaaggag
     1321 ccccgcccct tcgacctgct gttctggaac gccgaccaga cccgcatgcc caagtcgctg
     1381 cacatgttct acctgcgcaa tttctacaag gacaacgccc tgactaccgg cgccctcacc
     1441 ctgggcggcg agaagctgga cctctcgaag gtcaagattc cgatctatgt ccagtcctct
     1501 cgggacgacc acatcgcccc ctatcgcagc gtctatcgcg gcgcacgcgc ctttggcggt
     1561 cctgtgacct tcaccatggc cggctcgggc catatcgcgg gagtcatcaa ccatccggac
     1621 gccaagaagt accagcactg gaccaatggg gccctgcccg gctcggtcga ggactggatc
     1681 tccggcgcgc aggagcatcc ggggtcgtgg tggccgcact gggccgagtg gctgcgggcc
     1741 aggtccggcc ccctggtccc ggcccgcgat ccggccaaag gcaagctgaa gccgatcgag
     1801 gacgcgccgg gcagctatgt acgagtcaga tccgacgcct gatcgtcaaa aagagtcttc
     1861 agcgcaccct gttcgcccaa aactcgcccg gatcatgctt gagcttggcg acatgcccgc
     1921 gatcagtcgt cgaaccgtcc ttggaatgat tctggccgcg ccggcgttcg gcgcggcggc
     1981 gcccgcccga gacggcatgg gtctggctcg ggcggcgcgg gcgcagatcg gcgtgacgaa
     2041 ggcctatgac gccagctatc gcgctatcgc ctatccgcag ggcgacgttt tgcgcaccac
     2101 cggcgtatgc gccgacgtac tggtccgggc agcacgcgac gcctggaaga tcgacctgca
     2161 acagcgcatc cacgaggaca tggttcgtga cttcgatgcc tatccttccc gccggacctg
     2221 gggcctcaaa cgccccgacg ccaatatcga tcatcggcgt gtgctgaacc tggaaaccta
     2281 tctgacccgg caaggcgcct tgctgaagcg caacgccaag cgcgtcgccg gcgaccgctt
     2341 tgacgatccg ctgcccggcg acatcctgac ctggcgcgtc tggggcgagc gccctcacct
     2401 gggcatcgtc gcgcgcgggc ctgagggcgt gcgcgtggtc cacaatatcg gcttcgggac
     2461 caaggaagag ccgctgtgga tgttcaagct gcacggcgct gtcgcgcact accgctggcg
     2521 ggtgtgagct tgcggtaagg cacatgatgc cttactttca gaacccaagc ttccagcgag
     2581 gacgctatgg ccgccgaact gtcgatcacc gccaaggggc agatcaccct taaaaaatcc
     2641 gtactcgatc acctcggggt tagcccgggg cagaaggtgg atgtcaggct gttgcccaat
     2701 gggcgagttg aattgcggcc taccagcagc aaacgcgacc tctcaagcgt gttcggcatt
     2761 ctgaaaaggg agggtcagcg tacggtctcg atcgaggaaa tgcaggacgc gatcgaagct
     2821 ggcgcctgcg gcgagccgtg agggtcgcgc tcgacacgaa tgtcatcgtt cgatttctgg
     2881 tagtcgatga tgagccgcaa gcactggccg ctcgagctct ccttcacact gcggaccaga
     2941 ttctgatatc cacgctcgtt ttgtgtgaaa cggtctgggt tctgtcgcgg gcttaccgat
     3001 atcccgccgc ttccttggcc gacgccttgc gccaactgac agacatacag ggcgtggaaa
     3061 tcgatcgccc gatctttgag gccggcctcg ccatgctcga acgcggcggt gatttcgccg
     3121 atggctgtat cctgttcgag gcggggcgcg ctaaatgcga tagactggca accttcgacg
     3181 caggcctcat cgccctcagc cagggccgcg cctctcttcc ctaaaccacc ttaggtcgcg
     3241 ggggatcaca gacccgccgc ctcctccagg cccagcacca gattaaggtt ctgcaccgcc
     3301 gcgcccgagg cgcccttgcc cagattgtcg agcagggcca ccagacgcgc ctgaccactg
     3361 gtgtcggaac cgaacacgaa gaccttcagg cgattggttc cgttcaggcc ttccgggtcc
     3421 agggtggtca aacccttggc ctcgtccagc gaggcgacct cgacaaactg ttcgcccttg
     3481 tagtgggcgg cataggcggc atggatgtcg tgcaggcagc ggccatcttc cagcatcgcc
     3541 aggtgcaggg gcagctccac cagcatgccc tgagcatagc gacctacggc gggggtaaag
     3601 atcggtcggg tcagcaggcc gccatgcttc tggatctccg gcacatgctt gtgcgacagg
     3661 cccatggcgt agatccggta aggggcacgg gtgtagttcg gcgcagcttc gtcctcgaac
     3721 tcggcgatca tcgccttgcc gccgccggta tagccggaca cggcattgaa cgagagcggc
     3781 agctcggcgg gcaggatgcc ggccgagacc agcggccggg tcagggcgat ggcgcccgtc
     3841 gagtagcagc ccggattgga gatgcgcctg gacgcggcga tcttgccgcg ctgctcggcg
     3901 tcgagttcgg ggaaaccgaa cgcccagccc ggatcgatgc gataggccgt cgaggcgtcg
     3961 atcaccttga cgtcgggatt ggtgattagg ccgacggcct ccttcgaggc ctcatcgggc
     4021 aggcacagga tgaccgcgtc ggcgccgttc agcatggcgg cgcgggcggc cgggtccttg
     4081 cgcttgtcgg gatcgatcga gatcagctgc agatcggtgc ggccgaccag gcgctcgcgg
     4141 atctgcagac ccgtggtgcc gacttcgcca tcgatgaaga ccttgggagc catgctgacg
     4201 tccaatcaat ccagacaata gacaaagaaa agggcgctcc ggtttcccga agcgcccttt
     4261 tccaaacacg gtgaaccgcg tttagcgctt cgagaactgg aagctacgac gggccttggc
     4321 cttgccgtac ttcttgcgct caaccacgcg gctgtcgcgc gtcaggaagc cgtgcggctt
     4381 caggacggga cgcagcgagg gctcatagta ggtcagggcc ttggacaggc cgtggcgcac
     4441 cgcgccggcc tggcccgaca ggcccgagcc ttcaacggtg acgacgacat caaactgacc
     4501 caggcggtca gtgacctgca ggggctgagc gatcatcatg cgcagcaccg gacgcgcgaa
     4561 gtaaacttcc tggtcgcggc cgttgatggt gatggtgccc ttgccgggct tgatccagac
     4621 gcgagcgatc gcgttcttgc gcttgccggt cgcataggcg cggccttggg cgtcgatctt
     4681 ctggacggcc ggagcggccg cttcggggtt gctggacagg ctggccagcg cgtcaaagcc
     4741 ttgagcttcg gacatgactt aggcgc
//
LOCUS       DAOMEO010000077         4760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2216_length_4760_cov_2.315622, whole genome shotgun sequence.
ACCESSION   DAOMEO010000077 DAOMEO010000000
VERSION     DAOMEO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_4760_cov_2.315622"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>606
                     /locus_tag="PLH31_02740"
     CDS             <1..>606
                     /locus_tag="PLH31_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88190.1"
                     /translation="PVADLTAKATYGESFIAPSLGELNDPGRVSPVNVSNGSRTILTL
                     IRYGGNKDLRPETAKSWTAGLDYAPAAYPTLRLSGTLFRTAFKNRIGQPANDNPATVL
                     TAPDLAPFRTFISPTTNPADLALVQELLLGATAATAALYPATAYGAIADARYTNTGTF
                     TVRGLDLNGTYGLKIGDDPLTLSGNLSWLMSYRRKISASAQA"
     assembly_gap    607..616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <617..960
                     /locus_tag="PLH31_02745"
     CDS             <617..960
                     /locus_tag="PLH31_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88191.1"
                     /translation="LAGMTEYPADLRGRVSAAWTHGSLTTTASLNHVGDLHAASGQRL
                     KAMTTADLQLQYAPRVQSGPWHGVALALSAQNLFDQDPPFYDNRVGVAYDPANYDPFG
                     RVVALQLTKAW"
     gene            997..3444
                     /locus_tag="PLH31_02750"
     CDS             997..3444
                     /locus_tag="PLH31_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR88192.1"
                     /translation="MRAFAWLPLACVLASGEALAARPFTVEDLLSLREAGRAAFSPDQ
                     NWLVVETFAPWREAPRYDHDTLTSQTLGRLVVVDLKAGGAAKPLLATDPLAGDTLGAF
                     SPDGSRVIVFRLREHRRVLGIVTLATGAVVWTDLTVEPELWTAAARWRNDQEIVAISR
                     PPEAASRGLGGGWQPQARIAAARAAATAGHLSVAPLGSGRFTDLNPPADLARLALIEA
                     DSGQVRTLASGAFADLSVSPDGRTAAVIEEGALNPPIADGAIRTGDPPRQRRLTLVDL
                     ADGHRLTPCVTCDLQPYVWSWSPDGASLLAYVRAGGPAGPGYWRFSRAGAAPLAPGLL
                     AGETGGKDPRTQADAAWLGRDAVVLARPADKTRTDWWRLAAQGPVNLTAELSAPAGRR
                     LIAGSDGLVFTAGGQIWRIAERGAPRPVGGPETRLKPGPTLPGQAPVLALATDSQGAR
                     LHSASGDAGPAALLPSGARLLALGPNDAAAVLLSDPNGLGHIELRAPNRTPRPVLTLN
                     PDLADVAFAAPVRIDHAGPDGEALASWLYLPPGHRASNDRPIIVVPYPGARYGAPPEE
                     AAPGALSLPTNVQLMAAAGYAVLVPSLPLSPAREPMDGLADAMLRVVDAARAAQPGLS
                     ATRLAVWGQSYGGYGALAAGVQSPRFKAVIASAPITNLLSFYGAWPASLATLPEAGLP
                     IASQLAWSETGQGRMGVPPWRDPQRYLRNSPGLQTDRLAAPVMLIYGDLDFDIGQVQT
                     VFASLYRQGKDAQLLIYRGEGHVVLSPDNVRDLYGRAFAFLRATLGDPSALAVALPPS
                     PASDVASAAPAAIRPSQ"
     gene            complement(3456..4517)
                     /locus_tag="PLH31_02755"
                     /pseudo
     CDS             complement(3456..4517)
                     /locus_tag="PLH31_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
BASE COUNT          665 a         1752 c         1663 g          670 t
ORIGIN      
        1 gccggtcgcc gacctgaccg ccaaggccac ctatggcgag tcgttcatcg cgccctcgct
       61 gggcgaattg aacgatccgg gccgggtctc gccggtcaat gtcagcaacg gctcgcgcac
      121 gatcctgacc ttgatccgct atggcggaaa caaggacctg cgtcccgaga cggccaagtc
      181 gtggacggcc ggcctcgact acgccccggc cgcctatccg accttgcgcc tgtcggggac
      241 gctgtttcgg acggcgttca agaaccggat cggtcagccg gccaacgaca acccggctac
      301 cgtcctgacg gcgcccgatc tcgcgccctt ccgcaccttc atcagcccga ccaccaaccc
      361 ggccgatctg gcgctggtgc aggagctgct gctgggcgcg acagcggcga cggcggccct
      421 ctatccggcc acggcctatg gcgcgatcgc cgacgctcgc tacaccaaca ccgggacgtt
      481 caccgtccgg ggtctggacc tgaacgggac ctatgggctg aagatcggcg acgatcccct
      541 gaccctcagc ggcaacctct cctggctgat gtcctatcgg cgcaagatct cggcctcagc
      601 ccaggcnnnn nnnnnnaact ggccggcatg accgaatatc ccgccgacct tcgcggccgc
      661 gtctcggcgg cctggaccca cgggtcgctg accaccacgg ccagtctcaa ccacgtcggc
      721 gatctgcatg ccgccagcgg ccaacggctg aaggcgatga ccacggccga cctgcaactg
      781 cagtatgcgc ccagggttca gagcggtccc tggcatggcg tggccctggc cctgagcgcc
      841 cagaacctct tcgatcagga cccgccgttc tacgacaacc gggtcggggt cgcttatgac
      901 cccgccaatt acgacccgtt cggccgggtc gtggcgctgc aactgaccaa ggcctggtag
      961 gcctccaccg cacccccaaa tcttgaggag accgcgatgc gcgctttcgc atggcttccg
     1021 ctcgcctgcg tcctggccag cggcgaggcg ctggccgccc gtccgttcac cgtcgaagac
     1081 ctgctctccc tgcgcgaagc cggtcgggcg gcgttctcgc ccgaccaaaa ctggctggtc
     1141 gtcgagacct tcgcgccctg gcgcgaggcc ccccgctacg atcacgacac cctgacgtcc
     1201 caaaccctgg ggcgtctggt cgtggtcgat ctcaaggccg ggggtgcggc caagcccttg
     1261 ttggcgaccg atcccctggc cggcgacacc ctgggggcgt tttcgccaga cggatcgcgg
     1321 gtgatcgtct ttcggctgcg cgaacaccgc cgtgtcctgg gcatcgtcac cttggcgacc
     1381 ggcgccgtgg tctggaccga cctgaccgtc gagccggaac tgtggaccgc cgccgcccgc
     1441 tggcgaaacg accaggagat cgtggcgatc agccgaccgc ccgaggccgc gtctcgcggt
     1501 ttgggcggcg gctggcagcc gcaagcgcgg atcgcggccg cccgggcggc ggcgacggcg
     1561 ggccatctgt cggtggcgcc cttgggcagc ggccggttca ccgacctcaa tccgccggcc
     1621 gacctggcgc gtctggccct gattgaggcc gacagcggcc aggtgcgaac cttggccagc
     1681 ggcgccttcg ccgatctgtc ggtgtcgccg gacgggcgga cggcagccgt gatcgaagag
     1741 ggcgcgctga acccgccgat cgccgacggc gcgatccgca ccggcgatcc gccgcgccaa
     1801 cgccggctga cgctggtcga tctggccgac ggccatcgcc tgacgccgtg cgtgacctgc
     1861 gatctgcagc cctatgtctg gagctggtcg cccgatgggg catcgctact cgcctatgtc
     1921 cgggccggcg ggccggccgg tcccggctat tggcggttct cgcgcgcagg ggccgcgccg
     1981 ctcgcgcccg gcctgctggc cggcgagacc ggtggcaagg accctcgcac ccaggccgac
     2041 gctgcttggc tcggccgaga cgcggtcgta ctggcccgac cggccgacaa gacccggacg
     2101 gactggtggc ggctggccgc ccagggacca gtcaacctca ccgccgagct gtcggctccg
     2161 gccgggcgac ggctgatcgc gggatccgac ggcctggtgt tcacggccgg cgggcaaatc
     2221 tggcggatcg cggagcgcgg cgcgccgcgc ccggtcggcg gcccggagac ccgtctcaaa
     2281 cccggaccga ccctgcccgg ccaggcgcca gtcttggccc tggcgacgga cagccaaggc
     2341 gcgcggctgc attcggcctc cggcgacgcc ggccccgccg cgctgctccc gagcggcgcg
     2401 cgcctgctcg cccttgggcc aaacgacgcg gccgcggttc tgctcagcga tccgaacggc
     2461 cttggccaca tcgagctacg cgcgcccaac cgaacgccgc gtccggtcct gaccctcaac
     2521 cccgacctgg ccgacgtggc cttcgccgcg cctgtgcgca tcgaccacgc cggccccgac
     2581 ggcgaggccc tggccagctg gctctatctg ccgcctggtc atagggcgtc caatgatcga
     2641 ccgatcatcg tcgtgcccta ccccggcgcc cgctacggcg cgccgccgga ggaggccgcg
     2701 ccgggcgcct tgagcctgcc gaccaatgtc cagctgatgg ccgccgccgg ctatgcggtg
     2761 ctggtcccca gcctgccgct gtcgccggcg cgcgagccga tggatggcct agccgacgcg
     2821 atgctgcgag tggtcgatgc ggcgcgcgcc gcgcagccgg gcttgtcggc cacgcgcctg
     2881 gcggtctggg gccagtccta tggcggctat ggcgcgctgg cggccggggt tcagtcgccc
     2941 cgcttcaagg cggtgatcgc ctcggccccg atcaccaatc tgctgtcgtt ctacggggcc
     3001 tggccggcga gcttagcgac cctgccggag gccggcctgc cgatcgccag ccagctggcc
     3061 tggtcggaga ccggacaggg gcggatgggg gtgccgccct ggcgcgatcc ccagcgctat
     3121 ctgcgcaaca gccccggcct gcagaccgac cggctcgccg ccccggtgat gctgatctat
     3181 ggcgatctgg atttcgacat cggtcaggtc cagacggtgt tcgcctccct ctatcggcaa
     3241 ggcaaggacg cgcagctgct gatctatcgc ggcgaaggcc atgtggtcct cagtccggac
     3301 aatgtccgcg acctctatgg acgggccttc gctttcctgc gggcgacgct gggcgatccg
     3361 tcggcgctgg cggtcgcctt gcctccttcc ccagcatcag acgtcgcctc ggcggcgccg
     3421 gccgcgatcc gcccctccca gtgacgcagc caagcctcga cctgggcggc ggtcaggatc
     3481 gcgccatagt cgtcgcgcca gaccagggcc tccggcgcca aagccgcttc gagccgcgaa
     3541 cggtcgatca agcccagagc cgccagccgg ccttccagca ggaagggccg caggaaagac
     3601 aggctggcgg cgacgctgcg cccgaagaag acggtgacgt cgcccttcga ccgacggtgc
     3661 agaatagaag gcggcaagtg atcggcgaag gcggcccgcg cgaagctgcg gtcgccctct
     3721 ccggcgctga ggatcggggc cgggatcgcc agacagagct cgacgacggg ctgggtcagc
     3781 aacggatggg ccagtcgcgc gacctgcccg cgccggctgg ccgagaagac gctctggttg
     3841 ttgaccaggc cggcgatctg caggcgcttg gccggcgcga tgccgccgag gttggtcagc
     3901 cagggatgcg gcgcgccggg cgcccgacgg atgaagccgg tggccggccg gatcagggcg
     3961 ggcgagccct gccccggccg cctgtgcagc gcttcccaag ccaggctcca gaccgagcgc
     4021 cgggtgcgcc gggcgatgtc gcccaggcgg gccaggcgcg agcccgcgca agggacgccg
     4081 gctaacaggt cggcggcgat ctgggccgcg cccaactggt agaagaccac gtcgccgccg
     4141 tgaccggtga agagggcgtc ggccttggcc gtcttcaggg cctgggccag cagccggtca
     4201 tagtccggat cgatgccgtt gaaacccggc cgcgcggtcg ccgccccatt ggccagcttg
     4261 gcctgggtca ggacgaacgg tgcgcgccgc acgacctcga gcggcgtccc cgccagatcg
     4321 gcggccgcct gggcataaac ccgctcgtcg ctctcggccc ggtcgcgata gaagttgatc
     4381 gccgcgtccg gcggccggcc gatctgagcc aaggtcccgg cgacgatcgc cgagtccaga
     4441 ccgccggaga tctcgcagag gatccgcttg cggtcctggg tcaaggcggc gacgcaggcc
     4501 tcgacggtcc gccgcaggtc gtcggtcgta gggatcgcct tggcctgccg agcgacctgc
     4561 gccggcgtcc acagggtcag gacctcgcgg ccgcccggtc catgacggca ggtccccggc
     4621 gcgatcgcct cgaccccgac cagcggcagg gggccggcgg cgcgtggcgt atcggccagg
     4681 ataccggcga ggcgggaggg ggcgatggcc aggtcgcgcg gctggaaggc cccgtccggc
     4741 aggtccgagg caaccagggt
//
LOCUS       DAOMEO010000078         4747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2226_length_4747_cov_2.655797, whole genome shotgun sequence.
ACCESSION   DAOMEO010000078 DAOMEO010000000
VERSION     DAOMEO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2226_length_4747_cov_2.655797"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLH31_02760"
     CDS             complement(<1..616)
                     /locus_tag="PLH31_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR88193.1"
                     /translation="MTISIRSVEKKFGRYPALNKIGLEIADGELLALLGPSGSGKTTL
                     LRVIAGLEFPDAGQVLFHDQDVTFASAAARRVGFVFQQYALFKHMTVAKNIAFGLDVR
                     KGKDKPSRAEITRRVDELLRLVELEGLGGRYPSQLSGGQRQRVALSRALAVQPSVLLL
                     DEPFGALDATVRKSLRRELRRVHDATGVTTIFVTHDQEEALDLAD"
     gene            complement(634..1476)
                     /gene="cysW"
                     /locus_tag="PLH31_02765"
     CDS             complement(634..1476)
                     /gene="cysW"
                     /locus_tag="PLH31_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HQR88194.1"
                     /translation="MAAPRLSTEDPAWAKALIITLVLVFLGLVLVLPLAAVFAEALRK
                     GLQPALDAIANPDAIAAVKLTLLTAAITVPFNVVFGLCAAWAVAKHEFPGKSLLITLI
                     DLPFSVSPVVAGLIYVLVFGLQGWFGDHLVDNGVRIIFAVPGIVLATIFVTFPFIARE
                     LIPLMQEQGTSEEEAAVSMGASGLYTFWRVTTPNVRWGLLYGVLLCNARAMGEFGAVS
                     VVSGHIRGLTNTMPLHVEILYNEYDFVGAFAVAALLCLLAIVTLVLKTALEVAQPGAG
                     RRGH"
     gene            complement(1476..2345)
                     /gene="cysT"
                     /locus_tag="PLH31_02770"
     CDS             complement(1476..2345)
                     /gene="cysT"
                     /locus_tag="PLH31_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HQR88195.1"
                     /translation="MTAEAAAIRTGPAARRPLFRRRSAIPGFTLTLGVTLTVLSLIVL
                     IPLSAVALKAAELSPEQFVAAAFSERALAAYRLTFGAAFVAAAINGVFGVLTAWALVR
                     YDFPGKTLVNALVDLPFALPTAVAGIALATLYSPNGWIGAYLTPLGIKAAYNPIGVTI
                     ALIFIGLPFVVRTIEPVLRDLSNDVEEAAASLGAGRLSTIARIVLPALGPSWLTGFAL
                     AFARGVGEYGSVIFIAGNMPYKSEIAPLLIIIQLEQFEYANAAAIALVMLGVSFLMLL
                     VINAVQAWARRFS"
     gene            complement(2607..2681)
                     /locus_tag="PLH31_02775"
     tRNA            complement(2607..2681)
                     /locus_tag="PLH31_02775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2647..2649),aa:Lys,seq:ttt)
     gene            2807..3457
                     /locus_tag="PLH31_02780"
     CDS             2807..3457
                     /locus_tag="PLH31_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88196.1"
                     /translation="MRGFLLTALAIPLLLTGAPASAGQEAPPPSPADSKIQTTSDANK
                     ESLTGAVSAPLRDVNIIRTKIPPVLIEAMRDPYARPMRVNCAALIALVGPLNDALGED
                     LDAHPPEENEDLMDRGRKAAGTAALGAVASAAQDMIPMRGWVRKLSGAERHDRMVQSA
                     ITSGNVRRAYLKGLGEARGCNPPATPQHKPPAPIPVVDPGMPPPVEGFKPRFPIKN"
     gene            complement(3462..4574)
                     /gene="tgt"
                     /locus_tag="PLH31_02785"
     CDS             complement(3462..4574)
                     /gene="tgt"
                     /locus_tag="PLH31_02785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQR88197.1"
                     /translation="MAFPFEISATDGKARTGVLKTPRGDIRTPAFMPVGTAATVKAMT
                     VDQVKDTGADIILGNTYHLMLRPSAERVRRLGGLHKFMRWDKPILTDSGGFQVMSLSG
                     ISKVTEEAVTFASHIDGSKHVLSPERSMEIQADLLGSDIVMQLDECVAWPAEEKRARE
                     GMELSARWGIRSKAAFGTRDTQVLFGIQQGSTFENLRRESSQRLQDIGFDGYAIGGLA
                     VGEGHTAMCEVLDYAPDMLPADKPRYLMGVGKPIDLVEAVARGVDMFDCVLPTRSGRH
                     GQAWTWDGAINIKNARYADDESPLDPGSACSASRDYSKAYLRHLFKAEEILGQVLLSW
                     HNIAFFQALTSAMREAISEGRFEAFRRDFAARQSPV"
     gene            complement(4577..>4747)
                     /locus_tag="PLH31_02790"
     CDS             complement(4577..>4747)
                     /locus_tag="PLH31_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HQR88198.1"
                     /translation="LMTNFHLPKSTLFMLVSAFAGQKTMREAYERAIATGYRFYSYGD
                     SSLLFPAEPDET"
BASE COUNT          880 a         1566 c         1596 g          705 t
ORIGIN      
        1 ggtcagccag gtcgagggcc tcttcctggt cgtgggtgac gaagatggtg gtgacgccgg
       61 tggcgtcatg cacccggcgc agttccctgc gcagcgactt gcggaccgtg gcgtccaggg
      121 cgccgaacgg ctcgtccagc agcagcacgc tgggctgcac cgccagcgcc cgtgacaggg
      181 ccacacgctg acgctggccg ccggacagct gtgaggggta gcggccgccc agaccttcca
      241 gctcgaccag ccgcagcagc tcatcgaccc gccgggtgat ctcggccctg gacggcttgt
      301 ccttgccctt gcgaacgtcg aggccgaacg cgatgttttt ggccacggtc atatgcttga
      361 acagcgcata ttgctggaac acgaagccca cgcgccgcgc cgccgccgag gcgaaggtga
      421 cgtcctgatc gtggaacagc acctggccgg cgtcggggaa ttccaggccg gcgatcaccc
      481 gcagcagggt ggtcttgccc gagcccgaag gccccagcaa ggccagcaac tcgccatcgg
      541 cgatctcaag cccgatcttg ttcagggccg gatagcgacc gaacttcttt tcgacggaac
      601 ggatggagat ggtcatgagg tctggcgttc cgatcagtgc ccgcgccggc ccgcgccggg
      661 ctgagcgact tcaagcgcag tcttgagaac cagggtcacg atcgccagca ggcacagcaa
      721 ggcggcgacg gcgaaggccc cgacgaaatc gtactcgtta taaagaatct cgacgtgcag
      781 cggcatggtg ttggtcagcc cccggatgtg accgctgacc accgagacgg cgccgaactc
      841 gcccatggcc cgggcgttgc acagcaacac gccatacagc agcccccagc gcacgttcgg
      901 cgtggtgacc cgccagaagg tgtagaggcc cgaggcgccc atcgagacgg cggcctcttc
      961 ctcgctggtc ccctgctcct gcatcagcgg gatcagctcg cgcgcgatga aggggaaggt
     1021 cacgaagatg gtcgccagca cgatgcccgg cacggcgaaa atgatccgga cgccgttgtc
     1081 gaccagatgg tcgccaaacc agccctgcag gccaaacacc agcacataga tcaggcccgc
     1141 cacgacgggc gaaaccgaga acggcaggtc gatcagggtg atcagcaggc tcttgcccgg
     1201 gaactcgtgc ttggcgacgg cccaggcggc gcagaggcca aacaccacat tgaacggcac
     1261 ggtgatcgcg gcggtcagca gggtcagctt cacggcggcg atggcgtccg ggttggcgat
     1321 cgcgtccagc gccggctgca ggcctttgcg cagggcttcg gcgaacacgg cggccagagg
     1381 cagcacaagc accaggccca ggaacaccag gaccagggtg atgatcaggg ccttggccca
     1441 ggccggatct tcggtagaca ggcgcggcgc ggccatcagg agaaccgtct tgcccaggcc
     1501 tgaacggcgt tgatgaccag cagcatcagg aacgagacgc ccagcatcac aagagcgatg
     1561 gcggcggcgt tggcgtattc gaactgctca agctggatga tgatcagcag cggcgcgatc
     1621 tcggacttgt agggcatgtt gccggcgatg aagatcaccg agccgtactc gcccacgccg
     1681 cgggcgaagg ccagggcgaa ccccgtcagc cacgacggcc ccagggccgg caagacgata
     1741 cgggcaatgg tcgacaggcg ccccgcgccc aggctggcgg cggcctcctc cacgtcgttg
     1801 gacaggtcgc gcagcaccgg ctcgatggtg cgcaccacaa acggcagacc gatgaagatc
     1861 agggcgatcg tcacgccgat cgggttgtag gcggccttga tccccagcgg ggtcagatag
     1921 gcgccgatcc agccattggg cgaatagagg gtggccaggg cgatgccggc caccgccgtg
     1981 ggcagggcga acggcaggtc gaccagcgcg ttgaccagcg tcttgcccgg aaagtcgtaa
     2041 cgcaccagcg cccaggcggt cagcaccccg aacacgccat tgattgcggc ggcgacaaag
     2101 gccgcgccga aagtcagacg ataggccgcc agggcgcgct cggaaaaggc ggcggcgacg
     2161 aactgctcgg gcgacaactc ggcggccttc agggccacgg ccgagagcgg gatcagcacg
     2221 atcagcgaca gcaccgtcag ggtgacgccc agggtcaggg tgaagcccgg tatcgccgac
     2281 cgacgccgaa acagcggtct tcgagcggcg ggacccgttc tgatcgcggc ggcttcggcg
     2341 gtcatcgtct gatcgtcgtc ctcaatccgg cgaacacagg gcgcgggcgc gcccgctggc
     2401 tccgcttaaa tccctaccag cctagtgggg tcaattgaga attagcgagc gacggtcgca
     2461 gaaaaactca ccgcgcgcca tcgcggcggc aaaggactgg gaggggaacg catgggccag
     2521 aggcggcgtt tggatcggct ggaagcggcg ctctgctcgg atcggacgct gcgatgcgcc
     2581 ggccagcacc agaggctgac ggaatatggt aggccgggtg gggttcgaac ccacgaccat
     2641 tcgattaaaa gtcgaatgct ctaccactga gctaccggcc cgctcacgac gcggcgtggc
     2701 cggtggaagc ggcgcggaac atagtcgggg ggcgccgcag ccgcaagcgg ggtttcatcc
     2761 gttgtttgac atcacagcgc cgcttttggt gcgataaggt tcgggtatgc gtggttttct
     2821 cctcaccgcc cttgcgattc cgctcctgct gacaggcgcg cccgcctccg ccggccagga
     2881 agcgccgccg ccgtcaccgg ctgattcgaa aatccagacc acgtctgacg ccaacaagga
     2941 aagtctgacg ggcgctgtca gcgcgccgct gcgtgacgtg aatatcatcc gcaccaagat
     3001 ccctcccgtg ctgatcgagg ccatgaggga cccctatgcg cggccgatgc gcgtcaactg
     3061 cgccgcgctg atcgccctgg tgggtccgct caatgacgcc ctgggggaag acctggacgc
     3121 ccatccgcca gaggaaaacg aagacctgat ggatcgcggc cgcaaggcgg ccggcacagc
     3181 ggcccttggc gccgtggcca gcgccgccca ggacatgatc ccgatgcgcg gctgggttcg
     3241 caagctcagc ggcgccgagc ggcacgaccg gatggtgcag agcgccatca cctcgggcaa
     3301 tgtgcgccgg gcctatctga aagggctggg cgaggcccgc ggctgcaacc cgccggccac
     3361 gccgcagcac aagccgcccg cgccgatccc tgtggtcgat cccggcatgc cgccgcccgt
     3421 cgagggcttc aagccgcgct ttccgatcaa gaactagaga cttaaaccgg gctctggcgc
     3481 gcggcgaagt cgcggcggaa ggcctcgaac cggccctcgc tgatcgcttc ccgcatggcc
     3541 gacgtcaggg cctggaagaa agcgatgttg tgccaggaca acagcacctg acccaggatc
     3601 tcctcggcct tgaacaggtg gcgcagatag gccttggaat agtcgcggct ggccgagcag
     3661 gcgctgccgg ggtccagcgg cgactcgtcg tcggcatatc gagcgttctt gatgttgatc
     3721 gccccatccc acgtccaggc ctggccgtgg cggcccgagc gcgtgggcag cacacagtcg
     3781 aacatgtcga cgccccgcgc cacagcctcg accagatcaa tcggcttgcc cacgcccatc
     3841 aggtagcgcg gcttgtcggc gggcagcatg tcaggcgcat agtccagcac ctcgcacatg
     3901 gcggtgtgcc cctcgcccac cgccagcccg ccgatggcgt agccgtcaaa gccgatgtcc
     3961 tgcaggcgct gtgacgactc gcgacgcagg ttttcgaagg tcgagccctg ctggatgcca
     4021 aacagcacct gcgtatcccg cgtaccgaac gccgccttgg accgtatgcc ccaacgggcc
     4081 gacagctcca tgccctcgcg agcccgcttc tcctcggcgg gccaggcgac gcattcgtcc
     4141 agctgcatga cgatgtcgct gcccagcagg tcggcctgaa tctccatcga gcgctcgggc
     4201 gagaggacgt gcttggagcc gtcgatatgg ctggcgaagg tgacggcttc ctcggtgacc
     4261 ttgctgatcc ccgacaggct catcacctgg aagccgccgc tgtcggtcag gatcggcttg
     4321 tcccagcgca tgaacttgtg aagcccaccc agacgcctca cccgctcggc gctgggccgc
     4381 agcatcaggt gataggtatt gcccaggatg atgtcggccc cggtgtcctt gacctggtcg
     4441 acggtcatcg ccttgacggt ggccgccgtg cccaccggca tgaaggccgg ggtgcggatg
     4501 tctccacggg gcgtcttcag caccccggtg cgcgccttgc cgtccgtggc ggagatctcg
     4561 aaggggaacg ccatgctcaa gtctcgtcag gctctgcggg gaacagcagc gagctgtcgc
     4621 cataggaata gaagcgatag cccgtggcga tggcccgctc ataggcctcc cgcatggtct
     4681 tctggccagc aaaggcgctg accagcatga acagggtcga cttgggcagg tggaaattgg
     4741 tcatcag
//
LOCUS       DAOMEO010000079         4744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2229_length_4744_cov_2.505865, whole genome shotgun sequence.
ACCESSION   DAOMEO010000079 DAOMEO010000000
VERSION     DAOMEO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_4744_cov_2.505865"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLH31_02795"
     CDS             complement(<1..439)
                     /locus_tag="PLH31_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HQR88199.1"
                     /translation="MNQAALKLVGKEDGDKQRALEAALAQIDRAFGKGSVMKLGEKGK
                     VEMESVSTGSLGLDIALGIGGLPKGRIIEIYGPESSGKTTLALHVVAEVQKAGGTAAF
                     VDAEHALDPGYAFKLGVNLDNLLVSQPDNGEQALEITDTLVRSG"
     gene            694..>2237
                     /locus_tag="PLH31_02800"
     CDS             694..>2237
                     /locus_tag="PLH31_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR88200.1"
                     /translation="MSIDPRRLLGLAFASADLLIEIRDGKIAMALGAAQRILGQSEAA
                     LTGKVWRDLFAPADQALMDAIVACADDGARRGPVVIRLAGPVERYVSVVMRALPENGG
                     RISCALTSSQAPSPAVGPEGLHPRESFEDIAKGLFEAARVTGIELELAMIEFAGLGER
                     RAALPAAEAEVLDAQVAGAVRAESHGGAAATRLSSDRFALLRAKDENPENLTRRLTQM
                     VDLDATAHVVSLEVGSASARAMRALRYAMDDFLREGLKDAPPMSLAEAMNRSVKRTLA
                     RAGALGVAVSQRRFNLAFQPVVNLATGVAHHHEALVRFEDGVSPFALIRMAEEFDLIE
                     ELDHAIVEQAVRRLAQDRSGELKLAVNVSGRTIINESFVDNVSRTLAKFDKAKGRLIF
                     EITESAAIDDLPRANRHIQALRGLGSMVCLDDFGAGAASFAYLQELNLDIVKIDGRYV
                     RELAGNGRDGAMVRRLVELCRDLKIRTVAEMVETVEVEEVVRNSGVDFAQGWLFGRPA
                     DQPQPAL"
     assembly_gap    2238..2247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2302..2437)
                     /locus_tag="PLH31_02805"
                     /pseudo
     CDS             complement(2302..2437)
                     /locus_tag="PLH31_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2444..4270)
                     /locus_tag="PLH31_02810"
     CDS             complement(2444..4270)
                     /locus_tag="PLH31_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HQR88201.1"
                     /translation="MQTRRQMLLSTVAAGFAAASAPSLARAQAASEQATRMNALFDDM
                     MVKLLRQSPEMATGLGLDKGDLAWTKGLLTDRSAEAIAAGAAQTRQQLAALRSIDRRA
                     LSGMDAVNYDTVEFVLAVQDEGNQKFTYAGGGSGAPYVLSQLTGSYQQMPDFLDTQHA
                     IETKADADAYLQRMEGFARLMDQETLIAKRDYADGVIPPDFVIDKALIQMKSFADTPT
                     ADAPLIKSIVRRTTEKKIEGDWAGEATKVYLNSVLPALKRQIALLESVRPKATHDAGV
                     WRLKDGADYYATSLKNYTTANLTPDEIHQLGLDMVKSIEAQADKIFKSMGMSKGTVGE
                     RMAELGKDVYDNTDAAKEQLIADLNVKAKWIEKQLPAYFGQLPKAPLEIRRVPKATEA
                     GAPAGYYSSPSLDGKRPGIYWINLRDTKEQAKYILSTLTVHEGVPGHHLQLSLSNEAK
                     GLPLIRKVIGFSGYTEGWALYSEELAVEMGVYKTDPRGHIGMLHDALFRAVRLVVDSG
                     MHHKKWSREQAIKYMAETMGDEESGSITEIERYCVWPGQACSYMIGKIVWLRAREKAR
                     KALGRKFDIREFHDAGLLSGMTPLTVLETVIDNYIATRKAGK"
     gene            complement(4384..>4744)
                     /locus_tag="PLH31_02815"
     CDS             complement(4384..>4744)
                     /locus_tag="PLH31_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HQR88202.1"
                     /translation="ARRKLTNKLIAAKAAARLAPFFDPQVRLIAGDGSLLMGSAEVIA
                     AFDAQFRDPDFIAYVRTGEGVELDSAGLRAAEHGRWVGSWRGAPDQSGTYLAVWKKVT
                     GQWVIESELYVTLDRGD"
BASE COUNT          723 a         1552 c         1592 g          867 t
ORIGIN      
        1 cgcccgagcg caccagggtg tcggtgatct ccagagcctg ttcgccgttg tcgggctgcg
       61 agaccagcag gttgtcgaga ttgacgccca gcttgaaggc gtagcccgga tccagggcgt
      121 gctcggcgtc aacgaaggcg gcggtgccgc cagccttctg gacctcggcc accacgtgca
      181 gggccagggt ggtcttgccc gagctttcag ggccgtagat ctcgataatc cgccccttgg
      241 gcagaccgcc gatacccagg gcgatatcca ggccgagcga gccggtggag accgactcca
      301 tctcgacctt gcccttctcg cccaacttca tcaccgagcc cttgccaaac gcccgatcga
      361 tctgggcaag cgccgcctcg agcgcgcgct gcttgtcgcc gtcttcctta cccacgagtt
      421 tcaaagccgc ctggttcatt ggagatgcgc ccttattcca tgattccacc cggccaagcc
      481 gggcttcgcg tttcgcgcgc ccatcgatcg gtggaacacc caaagtcgta ccggctttgt
      541 tctgtgttcg caagatgttc ttttcgagtg gcgtccatgt tcgattaccc ggatcgggga
      601 caagcggctc ttggccgcac gtcttttccg gttcgggaaa cacgtcctta agccccccat
      661 gcgacggttc cggtcgtatg ggggagaaac gccttgtcga tcgatccgcg tcgcctgctt
      721 ggcctggcgt tcgccagcgc tgatttgctc atcgagattc gcgacggcaa gatcgccatg
      781 gccctgggcg cggcccagcg gatcctgggc cagagcgagg cggcgctgac cggcaaggtc
      841 tggcgcgacc tgttcgcccc ggctgatcag gccctgatgg acgccattgt cgcctgcgct
      901 gacgatggcg cgcgccgggg acccgtggtg atccgcctgg ccggtccggt ggaacgttat
      961 gtcagcgtgg tcatgcgggc cctgccggaa aacggcgggc gcatatcgtg cgccctgacc
     1021 tccagccagg cgcccagccc ggcggtgggg cccgaaggtc tccatccgcg cgagtcgttc
     1081 gaggatatcg ccaaaggcct gttcgaggcg gcgcgggtca ccggcattga gctggaactg
     1141 gccatgatcg aatttgccgg cctgggtgag cggcgggccg ccctccccgc cgccgaagcc
     1201 gaggtgctgg acgcccaggt cgccggcgcc gtgcgcgccg agtctcacgg cggcgcggcg
     1261 gccacgcggc tgtcgagcga ccgtttcgcc ctgctgcgcg ccaaggacga aaatcccgaa
     1321 aatctgacgc ggcgtctcac ccagatggtg gatctggacg ccaccgccca tgtggtgtcg
     1381 ctggaggtgg gctccgcctc ggcccgcgcc atgcgcgcgc tacgctacgc catggatgac
     1441 ttcctgcgcg aaggcctgaa ggacgccccg cccatgagcc tggccgaggc gatgaaccgc
     1501 tcggtcaaac gcactctggc gcgggccggg gccctgggcg tcgcagtgag ccagcgtcgg
     1561 ttcaacctgg cctttcaacc ggtcgtcaac ctggcgacgg gtgtggccca ccatcacgag
     1621 gcgctggtgc gcttcgagga cggcgtcagt ccgttcgccc tgatccggat ggccgaggag
     1681 ttcgacctta tcgaggaact cgaccacgcc atcgtcgagc aggccgtgcg ccgcctggcg
     1741 caggaccggt ctggcgagct gaaactggcg gtcaatgtct cggggcgcac aatcatcaac
     1801 gagagcttcg tcgacaatgt gtcgcgcacc ctggccaagt tcgacaaggc caaggggcgc
     1861 ttgatcttcg agatcaccga gagcgccgcc attgacgacc tgccgcgcgc taaccggcac
     1921 atccaggcgc tgcgcggcct gggatccatg gtgtgtcttg acgattttgg cgctggggcg
     1981 gcgtccttcg cctatctgca ggagctcaat ctcgacatcg tcaagatcga cggccgctat
     2041 gtgcgcgagc tggccggcaa tggccgcgac ggggccatgg tgcgccgact cgtcgagctg
     2101 tgtcgcgacc tgaagatccg cacggtcgcc gagatggtcg agaccgtcga ggtcgaggag
     2161 gtggtgcgca actccggcgt cgactttgcc cagggctggc tgtttggccg gccggcggac
     2221 cagccgcagc cggccctnnn nnnnnnncca agccctcggc gcggcgcgcg ggggcgtcgg
     2281 agagctgggg ctgaagagac tctcctcccc ctcctggggg aggtggcgcg gcgcgaatac
     2341 gcgccgtgac ggagggggaa ccggcgcacg agcccttgcg cgccgcagcc ccctccaccg
     2401 cttcgcggtc cccctccccc acgagggggg aggagatttc agcctatttg ccggccttcc
     2461 gggtcgcgat gtagttgtcg atcaccgtct cgagaacggt cagtggggtc atgcctgaca
     2521 gcaagcccgc gtcgtggaac tcgcggatgt caaacttgcg gcccagggcc ttccgggcct
     2581 tctcgcgggc gcgcagccag acgatcttgc cgatcatgta gctgcaggcc tggcccggcc
     2641 agacgcagta gcgttcgatc tcggtaatcg agccgctttc ctcgtcgccc atggtctctg
     2701 ccatgtactt gatggcctgt tcgcggctcc acttcttgtg gtgcatgccg ctgtcgacca
     2761 ccaggcgcac ggcgcggaac agggcgtcgt gcagcatgcc gatatgaccg cgcggatcgg
     2821 tcttgtagac gcccatctcg acggccagct cttccgaata gagcgcccag ccttcggtat
     2881 agcccgagaa gccgatcacc ttgcggatca gcggcaggcc cttggcctcg ttcgacagcg
     2941 agagttgcag gtggtgaccc gggacgccct cgtgcaccgt cagcgtgctc aggatgtact
     3001 tggcctgctc cttggtgtcg cgcaggttga tccaatagat gccaggacgc ttgccatcca
     3061 gcgagggcga actgtagtag ccggccggcg cgccggcctc tgttgccttg ggtacgcggc
     3121 ggatttccag cggggccttg ggcagctggc cgaaataggc gggcagctgc ttttcgatcc
     3181 acttggcctt gacgttgagg tcggcgatca gctgttcctt ggccgcgtcg gtattgtcgt
     3241 agacatcctt gcccagctcg gccatgcgct cgccgaccgt gcccttggac atgcccatgg
     3301 acttgaagat cttgtcggcc tgggcctcga tcgacttgac catgtccaga cccagctggt
     3361 ggatctcgtc gggggtcagg ttggcggtcg tgtagttctt cagcgaggtg gcgtagtagt
     3421 cggcgccgtc cttcaggcgc cagacgccgg cgtcgtgagt ggccttgggc cgcacgcttt
     3481 ccagcagcgc gatctggcgc ttcagggcgg gcaggaccga gttcagatag accttggtcg
     3541 cctcgccggc ccagtcgcct tcgatcttct tttccgtggt gcggcgcacg atcgacttga
     3601 tcagcggggc gtcggccgtg ggcgtgtcgg cgaacgactt catctggatc agggccttgt
     3661 cgatcacgaa gtcgggcggg atcacgccgt cggcgtagtc gcgcttggcg atcagggtct
     3721 cctggtccat caggcgggcg aaaccctcca tccgctgcag gtaggcgtcg gcgtcggcct
     3781 tggtctcgat ggcgtgctgg gtgtcgagga agtcgggcat ctgctgatag gagcccgtca
     3841 actggctcag cacatagggc gcgcccgaac cgccgccagc ataggtgaac ttctgattgc
     3901 cctcgtcctg caccgccagg acgaactcga cggtgtcata attgaccgcg tccatgccgc
     3961 tgagggcgcg gcggtcgatg ctgcgcaagg cggccagttg ctggcgggtc tgggcggcgc
     4021 cggcggcgat ggcctcggcc gagcggtcgg tcagcagacc cttggtccag gccagatcac
     4081 ccttgtcgag acccaggccg gtggccattt ccggcgactg gcgcagcagt ttgaccatca
     4141 tgtcgtcgaa cagcgcgttc atccgggtcg cctgctcgct tgccgcctgg gcgcgtgcga
     4201 gcgaaggggc cgaggccgca gcgaagccgg cggcgacggt ggacagcagc atttggcggc
     4261 gagtttgcaa ggcgagaccc ctaaagacgc atttgttggg cgaaggcgta ggcccagacc
     4321 tcgatagccg taaattaatt tctgttaatg caacgccgcg ccgatcctct ggcggcgaga
     4381 cgctcagtcg ccgcgatcca gggtcacata gagctcgctc tcgatcaccc actggccagt
     4441 gaccttcttc caaaccgcga gataggtccc cgactgatct ggcgcgcccc tccacgaacc
     4501 gacccagcgc ccgtgctcgg ccgcacgcag gccggcgctg tcgagctcca caccctcccc
     4561 ggtgcggaca taggcgatga agtccgggtc gcggaactgg gcgtcaaagg cggcgatcac
     4621 ctcggccgat cccatcagca gcgagccgtc gccggcgatc agcctgacct gcgggtcgaa
     4681 gaacggcgcc agccgcgcgg cggcctttgc ggcgatcagc ttgttggtca gcttgcggcg
     4741 ggcg
//
LOCUS       DAOMEO010000080         4734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2236_length_4734_cov_2.738833, whole genome shotgun sequence.
ACCESSION   DAOMEO010000080 DAOMEO010000000
VERSION     DAOMEO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2236_length_4734_cov_2.738833"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /gene="xylD"
                     /locus_tag="PLH31_02820"
     CDS             <1..1494
                     /gene="xylD"
                     /locus_tag="PLH31_02820"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050401 - xylonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0042843 - D-xylose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase XylD"
                     /protein_id="HQR88203.1"
                     /translation="RRPTAALDRNLSYLGLVETLHGYPIDAVVLTTGCDKTTPAGIMA
                     ATTVNIPAIVLSGGPMLDGWHDGELVGSGTVIWRSRRKLAAGEINEEEFIQRAADSAP
                     SAGHCNTMGTASTMNAVAEALGLSLTGCAAIPAPYRERGQMAYRTGQRIVDLAYEDIK
                     PKDILTQKAFENAIALVAAAGGSTNAQPHIAAMARHAGLEITADDWRAAYDIPLIVNM
                     QPAGKYLGERFHRAGGAPAVLWELLQQGRLHGDALTVTGKTMAENLEGRETKDREVIF
                     PYAQPLAEKAGFLVLKGNLFDFAIMKSSVIGADFRARYLSDPGKEDIFEARAIVFDGS
                     GDYHARINDPALNIDEHCILVIRGAGPIGWPGSAEVVNMQPPDALLKRGIMSLPTLGD
                     GRQSGTADSPSILNASPESAIGGGLSWLRTGDTIRIDLNTGRCDALLDEATIAERRKE
                     GLPPVPETMTPWQEIYRAHTGQLETGGVLEFAVKYQDLAAKLPRHNH"
     gene            1598..2935
                     /locus_tag="PLH31_02825"
     CDS             1598..2935
                     /locus_tag="PLH31_02825"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR88204.1"
                     /translation="MRPRDSAGRRLTKFYRTRVNTYAFRRRPRWRTAAAVVAVVAAVG
                     AAGLTVSRCVAPKKPEQTAAVSAAPPALAPLVAPETSRGQIDYRVLDARIQAMMADKT
                     MQGLAIAVVEDGRLAFVKGYGRTAPEGGEPVKAETIFRWASLSKTVAGTLSAQLAQDG
                     VLSLTDPLGKFGTSLRLPNDAQRTLTVEDLLAQRTGLPKNAYDGWLEGGRDPRLIRES
                     LAAVPLLCPPGACYSYQNIAYDTIREVIETATRQPYAEEVRQRLFLPLGMTSTTLGME
                     SLQSARHWARPSRGGRPATLVDAYYRVPAAAGVNSNILDLARWMQAQMGLAPAVLPAG
                     LVADIQRSRVNTGRPYGNSPLGRSLERPGYGLGMRSFTYKGHRLVGHSGAVTGYRSTM
                     MFDPAARTGVVMLWNSEASLPFKLQAEVFDLAYRQPFTDWLELKGDEGAAIAP"
     gene            complement(2943..>3366)
                     /locus_tag="PLH31_02830"
     CDS             complement(2943..>3366)
                     /locus_tag="PLH31_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88205.1"
                     /translation="NFGIIRRITRKYEAARIDPAPAEWMERAPAYATPKALADYEGGE
                     DYSYIEFVGMTGEGMYSKTDLYALGPKFALPVYVILGEQDLVSTPEVARAWFDTIQAP
                     DKAFVLLPRTGHDPNPAMAAAQLEVLRSRVLPRIGKTN"
     assembly_gap    3367..3376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3377..4022)
                     /locus_tag="PLH31_02835"
     CDS             complement(<3377..4022)
                     /locus_tag="PLH31_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR88206.1"
                     /translation="MSKFLGLIVALVLSGAPGLALAQAPAKPPVSAAVKTIHEEGFVR
                     IGGIEQWVTLHGQDRTKPVILMAHGGPGNPMTPYANPYFKAWEKDFVIAQWDQRGAGM
                     TYGRNPPAEGEPLTVETLRDDGIEVANHVRQRLGQQKLILMGGSWSSILGTHMAKARP
                     DLFHAYIGSSHLARSADNLKASYERTLGLARAAEDQDAIGKLETIGPPPWINPRN"
     gene            complement(4133..>4734)
                     /locus_tag="PLH31_02840"
     CDS             complement(4133..>4734)
                     /locus_tag="PLH31_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQR88207.1"
                     /translation="ASYLATAYFRQSSKAVTSVTRDIGGGVLLTTRENLGESRNGGLE
                     LVANGRLTPKITYNVSANAFWNEIEAQALGFTGTRSGTTVSGRANVNWQVTPKDFVQV
                     NAFTSGKQLTPQGSRSAMQMVNLGYRRKMNDKLSFVVTAQDVLDTFKNDTVIDTPLLR
                     DRTERRPKVRAVFIGFTYLFGGGKQREPGFDFGGAQVGG"
BASE COUNT          767 a         1637 c         1570 g          750 t
ORIGIN      
        1 cgtcgcccga cggcggcgct ggatcgcaac ctctcgtacc tcggcctggt cgagaccctg
       61 cacggctatc ccatcgacgc cgtggtgctg accaccggat gcgacaaaac caccccggcc
      121 ggcatcatgg ccgccaccac ggtcaacatc ccggccatcg tcctgtcggg cgggccgatg
      181 ctggacggct ggcatgacgg cgaactggtc ggctcgggca cggtgatctg gcgctcgcgc
      241 cgcaagctgg cggccggcga gatcaacgag gaagagttca tccagcgggc cgccgacagc
      301 gccccctcgg ccggccactg caacaccatg ggcacggcct cgaccatgaa cgccgtggcc
      361 gaggccctgg gcctgtccct gaccggctgc gcggccatcc cggcccccta tcgcgagcgc
      421 ggccagatgg cctatcgcac gggccagcgg atcgtcgacc tggcctatga agacatcaag
      481 cccaaggaca tcctgaccca gaaggcgttc gagaacgcca tcgccctggt agcggcggcc
      541 ggcggctcga ctaacgccca gccgcacatc gccgccatgg cgcgtcatgc gggcctggag
      601 atcactgccg acgactggcg cgctgcctat gacatcccgc tgatcgtcaa catgcagccg
      661 gccggcaagt atctgggcga gcgcttccac cgggccggcg gcgcccccgc cgtgctttgg
      721 gagctgctgc agcagggccg cctgcatggc gacgcgctga ccgtcaccgg caagaccatg
      781 gccgaaaacc tcgaaggccg cgaaaccaag gatcgcgagg tgatcttccc ctacgcccag
      841 ccgctggccg aaaaggccgg cttcctggtg ctgaagggca acctgttcga tttcgcgatc
      901 atgaagtcca gcgtcatcgg cgccgacttc cgggcccgct atctgagcga tccgggcaag
      961 gaagacatct tcgaggcccg cgccatcgtg ttcgacggct ccggcgacta tcacgcccgc
     1021 atcaacgatc cggccctgaa catcgacgag cactgcatcc tggtgatccg cggagccggt
     1081 cccatcggct ggccgggttc ggcggaagtc gtcaacatgc agccgccgga cgccctgctg
     1141 aagcgcggca tcatgagcct gccgaccctg ggcgacggcc gccagtcggg caccgccgac
     1201 agcccctcga tcctcaacgc ctcgcccgaa agcgccatcg gcggcgggct gtcgtggctg
     1261 cgcaccggcg acaccatccg catcgacctc aataccggcc gctgcgacgc cctgttggac
     1321 gaggcgacca tcgccgaacg ccgcaaggaa ggcctgccgc ccgtgccgga aaccatgacc
     1381 ccctggcagg aaatctaccg cgcccatacg ggccagctgg agaccggcgg ggtgctggag
     1441 tttgcggtca aataccagga tctggcggcc aagctgccgc gccacaacca ctgatcatca
     1501 tcaggcctcc ggagcgatcc ggaggccttt tgatttgcgg cggcggcctt tgtctcgccg
     1561 cagcaaaggg gtaggtggaa gtctccacga tgcggtgttg aggccgcgcg attcggcggg
     1621 cagacggctg accaagttct acaggacccg ggtaaacacc tacgcgtttc ggcgtcggcc
     1681 gcgctggcgg acggcggctg cggtcgtcgc tgtggtggcg gcggtcggtg cggctggcct
     1741 gacggtgtct cgctgcgtcg cgccgaaaaa gccggaacag acggctgcgg tttcggcggc
     1801 ccctcccgcc ctcgccccac ttgtggcgcc cgagacctcg cgcggccaga tcgactatcg
     1861 cgttctggac gcccgcatcc aggcgatgat ggccgacaag accatgcagg gcctggccat
     1921 cgccgtggtc gaggacggcc gcctcgcgtt cgtcaagggc tatggccgca ccgcgcccga
     1981 ggggggcgag ccggtcaaag ccgagaccat cttccgctgg gcttcgctgt ccaagaccgt
     2041 ggccggcacg ctcagcgccc agctggccca ggacggggtg ctgtcgctga ccgacccgct
     2101 gggcaagttc ggcaccagcc ttcgcctgcc aaacgacgcc cagcgcaccc tgacggtcga
     2161 ggacctgctg gcccagcgca cgggcctgcc caagaacgcc tatgacggct ggctggaggg
     2221 cggacgcgac cccagactga tccgcgagag cctggcggcg gtgccgctgc tgtgcccgcc
     2281 cggcgcctgc tacagctatc agaacatcgc ctatgacacg atccgcgagg tcattgagac
     2341 cgccactcgc cagccctatg ccgaggaagt gcgccagcgc ctgttcctgc ccctgggcat
     2401 gaccagcacc accctgggca tggagagcct gcagtcggcc aggcactggg cgcggccgtc
     2461 gcgcggcggt cgcccggcga cgctggtcga cgcctattat cgggtgccgg cggcggcggg
     2521 ggtcaattct aacatcctgg atctggcgcg ctggatgcag gcccagatgg gcctggcgcc
     2581 ggccgttctg ccagcgggac tggtggccga tatccagcgt tcccgcgtca ataccggccg
     2641 tccctatggc aattccccgc tgggccgctc gctggaacgc ccgggctatg gcctgggcat
     2701 gcgcagcttc acctacaagg gccaccgcct ggtcggccac agcggcgccg tgaccggcta
     2761 tcgctcgacc atgatgttcg acccggcggc caggaccggc gtggtgatgc tgtggaacag
     2821 cgaggccagc ctgcccttca agctgcaggc cgaggtcttc gacctggcct accgccagcc
     2881 cttcaccgac tggctggaac tgaagggcga cgaaggggcg gcgatcgcgc cctgacggcc
     2941 cttcagttcg ttttgccgat gcggggcagc accctgctcc tcaggacttc cagctgggcc
     3001 gccgccatgg cggggttggg gtcgtggccg gtgcggggca gcagcacaaa ggccttgtcg
     3061 ggcgcctgga tcgtgtcgaa ccaggcccgg gccacctcgg gcgtcgagac caggtcctgc
     3121 tcgcccagga tgacatagac cggcagggcg aactttggcc ccagggcgta gaggtcggtc
     3181 ttcgaataca tgccctcgcc ggtcatgccg acaaactcga tataggaata gtcctcgccg
     3241 ccctcatagt cggccagggc cttgggcgtg gcgtaggcgg gggcgcgctc catccattcg
     3301 gccggcgcgg ggtcgatccg ggccgcctcg tacttgcgcg tgatgcggcg aataatcccg
     3361 aagttgnnnn nnnnnnagtt gcgcggattg atccagggtg gcgggccaat cgtctccagc
     3421 ttgccgatcg cgtcctgatc ttcggcggcg cgcgccaggc ccagggtgcg ctcgtagctg
     3481 gccttcagat tgtcggccga gcgcgccaga tgcgacgagc cgatataggc atgaaacagg
     3541 tcgggccgag ccttggccat gtgcgtccct aggatagaac tccacgaccc gcccatcagg
     3601 atcagctttt gctggcccag acgctggcgg acatggttgg ccacctcaat cccgtcatcg
     3661 cgcagcgtct cgacggtgag aggctcgccc tcggcgggcg ggttacggcc ataggtcatg
     3721 cccgcgccgc gctggtccca ctgggcgatg acgaagtcct tctcccacgc cttgaagtag
     3781 gggttggcgt agggcgtcat cggattgccc ggaccgccgt gcgccatcag gatcacaggc
     3841 ttggtgcggt cctggccgtg gagggtgacc cactgctcga tcccgccgat gcggacgaag
     3901 ccttcctcgt ggatggtttt gaccgccgcc gagacaggag gtttcgcggg cgcctgcgcc
     3961 agggcaaggc ccggcgcgcc ggacagcacc agcgccacga tcagacccag aaacttcgac
     4021 atcacgccct cacaagcccg atgagatcgc tatcgactca tcgtccaccc atcactgacc
     4081 taacggtttc cgggcgcgga cgatatcgcc cgttgtctgg ccggccggcg ccctagccac
     4141 ccacctgcgc gccgccaaag tcgaagccag gctcgcgctg cttgccgccg ccgaacaggt
     4201 aggtgaagcc gatgaacacc gcgcgcacct tgggccggcg ctcggtgcgg tcgcgcagca
     4261 acggggtgtc gatgaccgtg tcgttcttga aggtgtcgag cacgtcctgg gccgtcacca
     4321 cgaaggacag cttgtcgttc atcttccgcc gatagcccag attgaccatc tgcatcgccg
     4381 aacgcgaacc ttgaggggtc agctgcttgc ccgaggtgaa ggcgttgacc tggacgaagt
     4441 ccttgggcgt gacctgccag ttgacgttgg cccggcccga cactgtcgtg cccgaccgcg
     4501 tgccggtaaa gcccagggcc tgggcctcga tctcgttcca gaaggcgttg gcgctgacat
     4561 tgtaggtgat cttgggcgtc agccggccat tggccaccag ctccagaccg ccattgcggc
     4621 tttcgcccag gttctcacgg gtagtcagca gcaccccgcc gccgatgtcg cgggtgacgc
     4681 tggtgacggc cttggagctc tggcggaaat aggcggtggc cagatacgag gccg
//
LOCUS       DAOMEO010000081         4729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2241_length_4729_cov_2.363500, whole genome shotgun sequence.
ACCESSION   DAOMEO010000081 DAOMEO010000000
VERSION     DAOMEO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_4729_cov_2.363500"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="folE"
                     /locus_tag="PLH31_02845"
     CDS             complement(<1..447)
                     /gene="folE"
                     /locus_tag="PLH31_02845"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HQR88208.1"
                     /translation="MDALTPESALLERCACAADDLQHSLDARPSREQALAAVRTLLAW
                     AGDNPNREGLLDTPQRVVDAYGEWFAGYEGDPAKELSRTFEDVQGYDDMVMLRGIDVQ
                     SHCEHHMAPFLGKAYVAYIPTDKVVGISKIARVVEIFSKRLQTQETL"
     gene            639..2030
                     /gene="cysS"
                     /locus_tag="PLH31_02850"
     CDS             639..2030
                     /gene="cysS"
                     /locus_tag="PLH31_02850"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQR88209.1"
                     /translation="MTFKLHDTLTREKRAFVPADPSRVTMYVCGPTVYNHAHIGNFRP
                     VVVFDVLYRMLRHIYGEDAVVYARNVTDVDDKINKKAFEEGVPIKTITDRYLAAYHAD
                     ADGLLALRPNLEPKATDHIDEIVAMIGKLIDNGSAYAAEGHVLFDTQAFPDYGQLSGR
                     NMDDMIAGARVEVAPYKRHPADFVLWKPSKENEPEWDSPWGAGRPGWHIECSAMIDAS
                     LGQTIDIHGGGIDLAFPHHENEVAQSRCAHDLPVLANYWLHNGFLDMSGEKMSKSLGN
                     VILPHELLKTTPGEVIRWALLSAHYRQPLDWTPELLEQSRKALDRLYGALRRAKDVPM
                     DQALEAPAEVIAALEDDLNTPQAISCFFEVSSAIERAVTAGDTVAIAANKARLIEAGA
                     LLGFLQADPDSWFEGDASDDLKAQVEDLLKRRVEARTAKDWTAADAIRAELDALGVVV
                     MDGPAGATWRMKD"
     gene            2035..2796
                     /locus_tag="PLH31_02855"
     CDS             2035..2796
                     /locus_tag="PLH31_02855"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HQR88210.1"
                     /translation="MKLEDQIVLISGAGRGLGAAIARAFASQGACVVINYRRSEAAAF
                     ALAAELGDRAVAIQADVSDREETRRLVNAAAEHFGSHVTTVVNNALGDFSFNGDGRET
                     IQSIPWEDFQGQLDVSLRGALNLIQATAPAMENAGFGRVINIGTNLFQNPVVPYHDYT
                     AAKAALLSLTRTAAADLGPGGITVNMLSGGLLRTTDASSATPEAIFDLIAQSTPLRRV
                     TTPAEFADAVLFFASPWSRAVTGQNLVVDGGLVRD"
     gene            2806..3276
                     /locus_tag="PLH31_02860"
     CDS             2806..3276
                     /locus_tag="PLH31_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HQR88211.1"
                     /translation="MQRAVEHRIGIQAPAELVWELVSDFSTWEHWNPVHPRAEGQLKI
                     GTALTVHQALPGEPVRVIQPVVQDWVPYEQLHWRSTRLGGFVTAIRYIEIENMGPSNS
                     TFSNGELFMGMLVRWVSKEERRKLRAAYAQMGEALRDRAETLWSQRQQDPIQTP"
     gene            3273..3734
                     /locus_tag="PLH31_02865"
     CDS             3273..3734
                     /locus_tag="PLH31_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQR88212.1"
                     /translation="MIDELYSAKILTLVANIPRAGRLAAPDASSEKTAKLCGSKITVD
                     VTLEGGKVSDFAQEVAACALGQASAAILGANIIGADLSEIELARDSLRAMLKTNGLPP
                     AGRFAELAVLEPVKAYPARHASTLLAFEAAVEAVSRATAATETRTSSAGAA"
     gene            3787..4041
                     /gene="yidD"
                     /locus_tag="PLH31_02870"
     CDS             3787..4041
                     /gene="yidD"
                     /locus_tag="PLH31_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQR88213.1"
                     /translation="MTFYDRTVDLGLRVYKLTLSPLIGRQCRFMPSCSEYAAQALKDH
                     GPAKGSWLALRRICRCNPFGGSGFDPPPPRDQPRKWTCEE"
     gene            4038..>4729
                     /locus_tag="PLH31_02875"
     CDS             4038..>4729
                     /locus_tag="PLH31_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HQR88214.1"
                     /translation="MIDLVFPDGSSRQYPAGSSGRDVAAAISKSLEKKALLIKLDGVL
                     LDLDRPLTPDLLTGERRIEIITRESPDALEVIRHDTAHVLAEAVQELFPGTQVTIGPN
                     VEDGFYYDFARDEPFSLDDLPRIEERMRQIVDRDEKIRREEVDRDAAIADFEALGESY
                     KAQIIRDLPASDTITVYHQGEKWKDLCRGPHLPSTKAVGKAFKLTKLAGAYWRGDQNN
                     AQLQRIYGTSWAT"
BASE COUNT          826 a         1602 c         1528 g          773 t
ORIGIN      
        1 cagggtctcc tgggtctgca ggcgtttgga gaagatctcg accacccggg cgatcttgga
       61 gatgccgacg accttgtcgg tcgggatgta ggcgacatag gccttgccga ggaagggggc
      121 catgtggtgc tcgcaatggc tctgcacgtc gatgccgcgc agcatgacca tgtcgtcata
      181 gccctgcacg tcctcgaagg tgcgggagag ctccttggcc gggtcgccct catagccggc
      241 gaaccactcg ccataggcgt cgaccacgcg ctgcggcgta tcgagcaggc cctcacggtt
      301 gggattgtca ccggcccagg ccagcagggt gcgcacggcg gccagggcct gttcgcggct
      361 cgggcgcgcg tcgaggctat gctggaggtc gtcggcggcg cacgcgcagc gctcgagcag
      421 ggcggattca ggcgtcaaag cgtccatatc ggcagggtct ttccacgggc tgagttgcgc
      481 cggagcgcct ctcgccccgg aatgacgcgt gccacccgtc tcacagctac ggtcgagatc
      541 gcccaaaaga tgacgtgtcg tccgcgtgcc cagtggctat atgggaccct tccaaccctc
      601 ggcaacacct cccgttacgt caggcagacg cgttcctcat gacctttaag ctccacgaca
      661 ccctgacgcg cgaaaagcgc gccttcgttc ccgccgatcc gagccgggtg acgatgtatg
      721 tctgcgggcc cacggtctat aaccacgccc atatcggcaa cttccggccg gtggtggttt
      781 tcgacgtgct gtaccggatg ctgcgccaca tctatggcga ggacgcggtg gtctatgcgc
      841 gtaacgtcac cgacgtggac gacaagatca acaagaaggc cttcgaggaa ggcgtgccga
      901 tcaagacgat caccgatcgc tatctggccg cctatcacgc tgacgccgac ggcctgctgg
      961 ccctgcgccc caacctcgag cccaaggcca ccgaccatat cgacgagatc gtcgcgatga
     1021 tcggcaagtt gatcgacaac ggatcggcct atgccgccga gggccatgtg ctgttcgaca
     1081 cgcaagcctt ccccgactac ggccagctct cgggccgcaa catggacgac atgatcgccg
     1141 gtgcccgggt cgaggtcgcg ccctacaagc gccaccccgc cgacttcgtg ctgtggaagc
     1201 cgtccaagga aaatgaaccc gagtgggaca gcccctgggg cgcggggcgt ccgggctggc
     1261 acatcgaatg ctcggccatg atcgacgcct cgctgggcca gaccatcgac atccatggcg
     1321 gcggcatcga cctggccttc ccgcaccacg agaacgaagt cgcccagagc cgttgcgccc
     1381 atgacctgcc ggtcctggcc aactactggc tgcacaacgg cttcttggat atgtccggcg
     1441 agaagatgtc caagagcctg ggcaatgtca tcctgcccca tgagctgctg aagaccacgc
     1501 ccggcgaagt gatccgctgg gccctgctca gcgcccatta ccgccagccg ctggactgga
     1561 cgcccgagct gctggagcag tcgcgcaagg cgctggatcg cctgtacggc gccctgcgcc
     1621 gcgccaagga cgtgccgatg gaccaggcgc tggaagcccc ggccgaggtg atcgccgcgc
     1681 tggaagacga tctcaacacg ccgcaggcga tctcgtgctt cttcgaggtc tccagcgcca
     1741 tcgagcgggc tgtcaccgcc ggtgacactg tcgccatcgc cgccaacaag gcccgcctga
     1801 tcgaggccgg cgccctgctg ggcttcctgc aggccgaccc ggacagctgg ttcgagggcg
     1861 acgcttccga cgacctgaag gcccaggtcg aggatctgct gaagcgccgg gtcgaggctc
     1921 gaaccgccaa ggactggacc gccgccgacg ccatccgcgc cgaactggac gccctgggcg
     1981 tggtggtcat ggatggcccg gccggcgcga cctggcggat gaaggactga tcgcttgaaa
     2041 ctggaagacc agatcgttct gatctccggc gcgggccggg gactgggcgc ggccatcgcg
     2101 cgggccttcg ccagccaggg cgcgtgcgtg gtcatcaact atcgccgcag cgaggccgcg
     2161 gccttcgccc tggcggccga gctgggcgac cgcgccgtgg cgatccaggc cgatgtcagc
     2221 gatcgcgaag agacccgccg gctggtcaac gccgccgccg agcatttcgg cagccacgtc
     2281 accacggtgg tcaacaacgc gctcggcgat ttcagcttca acggcgatgg ccgcgaaacc
     2341 atccagtcga tcccgtggga ggatttccag ggtcagctgg acgtgtcgct ccgcggcgcg
     2401 ctgaacctca tccaggctac ggcgccggcc atggaaaacg ccggctttgg ccgggtgatc
     2461 aacatcggca ccaacctgtt tcagaacccg gtggtgccct atcacgacta caccgccgcc
     2521 aaggccgcgc tgctgtcgct gacccgcacg gcggcggcgg atcttggtcc cggcgggatc
     2581 accgtcaaca tgctgtcggg cggcctgctg cgcaccaccg acgccagctc ggccaccccc
     2641 gaagcgatct ttgacctgat cgcccagagc acgccgcttc gccgggtgac gaccccggcc
     2701 gagttcgccg acgcggtgct gttcttcgcc tcgccctgga gtcgcgccgt caccggccag
     2761 aatctggtgg tcgacggcgg tctggtgcgg gactaaaagc ccgccatgca aagagccgtc
     2821 gaacatcgca ttggcatcca ggcgcccgcc gaactggtct gggagctggt ttccgacttc
     2881 tcgacctggg agcactggaa ccccgtccat ccccgggccg aggggcagct gaagatcggc
     2941 acggcgctga ccgttcatca ggccctgcct ggcgaaccgg tgcgggtgat ccagccggtc
     3001 gtgcaggact gggtgcccta tgagcaactt cactggcgct cgacccggct gggcggtttc
     3061 gtcaccgcga tccgctatat cgagatcgag aacatgggcc ccagcaactc gacgttctcg
     3121 aacggcgagc tgttcatggg catgctggtg cgctgggtca gcaaggaaga gcgccgcaag
     3181 ctgcgcgcgg cctatgccca gatgggcgag gccctgcgcg accgggccga aacgctctgg
     3241 tcgcaacgcc agcaggaccc catccaaacg ccatgatcga cgagctctac agcgccaaga
     3301 tcctgaccct ggtcgccaat atcccgcgcg ccgggcggct ggccgcgccc gacgcctcgt
     3361 cggagaagac cgctaagctg tgcggcagca agatcacggt ggatgtcacg ctggaaggcg
     3421 gcaaggtcag cgacttcgcc caggaggtcg cggcctgcgc cctgggtcag gccagcgccg
     3481 cgatcctggg cgccaacatc attggcgcag atctttccga gatcgagttg gcgcgggatt
     3541 ccctacgcgc tatgctgaaa acgaacggtc tgcctcctgc gggccggttt gcggagcttg
     3601 ccgtgctgga gcctgtaaag gcctatcccg cccgtcacgc ctcgacgctt ctggcgttcg
     3661 aggctgcggt ggaggctgtc tctcgggcca cagccgcgac ggaaacgcga actagcagcg
     3721 ccggcgccgc ttgattgcgg cttttcgcgg gtgcgataac caccctcgaa acgattgcgc
     3781 gctggcatga ccttttacga ccgcaccgtc gaccttggcc tccgggtcta caagttgacg
     3841 ctctcgcccc tcattgggcg tcagtgccgt ttcatgccga gttgttcgga gtatgcggct
     3901 caggcgctca aggatcacgg acccgccaag gggtcctggc tggctttgcg acggatttgc
     3961 cgatgcaatc cgtttggagg atcgggcttc gacccgcctc cgccgcgcga ccagccacgc
     4021 aagtggacat gtgaagaatg atcgatctgg tttttcctga cggttcgtcc cgtcaatatc
     4081 ccgcgggctc cagcgggcgc gacgtggcgg ccgccatttc caagtcgctg gaaaagaagg
     4141 cgctgctgat caagctggac ggcgtgctgc tcgaccttga tcgcccgctg acgcccgacc
     4201 tgctgaccgg cgagcgcagg atcgagatca tcacgcgcga gtcgcccgac gccctggagg
     4261 ttatccgcca cgataccgcc cacgtgctgg ccgaggccgt gcaggagttg ttccccggca
     4321 cccaggtgac catcggtccc aatgtcgagg acggcttcta ttacgacttc gcccgcgacg
     4381 agccgttcag cctggacgat ctgcccagga tcgaagagcg catgcgccag atcgtcgatc
     4441 gcgacgagaa gatccgccgc gaagaggtcg accgcgacgc cgccatcgcc gacttcgagg
     4501 ccctgggcga aagctacaag gcccagatca tccgcgatct gccggccagc gacaccatca
     4561 cggtctatca ccagggcgaa aagtggaagg acctgtgccg cgggccgcac cttccctcga
     4621 ccaaggcggt gggcaaggcc ttcaagctga cgaagctggc cggagcctac tggcgcggcg
     4681 accagaacaa cgcccagttg cagcggatct acggcacctc gtgggccac
//
LOCUS       DAOMEO010000082         4701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2255_length_4701_cov_2.155833, whole genome shotgun sequence.
ACCESSION   DAOMEO010000082 DAOMEO010000000
VERSION     DAOMEO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_4701_cov_2.155833"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1769)
                     /locus_tag="PLH31_02880"
     CDS             complement(186..1769)
                     /locus_tag="PLH31_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HQR88215.1"
                     /translation="MRTLIQTLGALALTAALCASTALGPAAAAEEIPRLARRGDATQL
                     LVDGKPFLILGGELGNSTASSLPYLQKQWPTLKALNLNTVLAPVQWDQVEPVEGRFDF
                     TVVDGVIAQARASDMRLVLLWFGAWKNSMSTYTPAWVKRDGQRFPLARSAQGQAQEIL
                     SPAAPATLAADARAFAALMAHLKAVDGDRHTVIMVQVENEIGMLPSARDHSPAAEAEF
                     RKPVPAAVLKATGKTGSGNWEQVFGKSPATDELYQAWTYGRFVEGLARAGASAYPLPM
                     YVNAALNKPGVAPGGYPSAGPLPHLYAVWKAAAPSVFQAPDIYYPDFTHWAAQYDRPD
                     NPLFLPEADRAGKVEAPANAFWAIGEHDAIGFSPFAIETISDPAGNPLTGAYALLDEL
                     TPVLLAHQGKGVTRGFRPPVSFEGVVDETPQTAKMGDWTVKVSFVDPWTPRDKQVFTA
                     HGGLVIRLSADEFLVAGSGITLTFGEGVGLEKVTEGRFVDGAWVEGRWLNGDETHQGR
                     HVRLEPGRFQTQRVKLYRY"
     gene            complement(1832..2191)
                     /locus_tag="PLH31_02885"
     CDS             complement(1832..2191)
                     /locus_tag="PLH31_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88216.1"
                     /translation="MVPRHALIIEDEILIALEVEALLREQGFTSVDVADNAREALDLA
                     LRRKPDLITADYRIIGGTGEDAVSAIMAHLGPIPVVYVTGNASCLSSRATNTVVEKPV
                     SPTRLARACQMAIQTAA"
     gene            complement(2313..>2491)
                     /locus_tag="PLH31_02890"
     CDS             complement(2313..>2491)
                     /locus_tag="PLH31_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88217.1"
                     /translation="GAVRILPRCQGFGALIQSGWRAYPIKAPTVRVPPPVVVTRTPLD
                     QVKQKLPWGADRKK"
     assembly_gap    2492..2501
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2502..2987)
                     /locus_tag="PLH31_02895"
     CDS             complement(<2502..2987)
                     /locus_tag="PLH31_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HQR88218.1"
                     /translation="MRPIRTALLAAAALLGASILASCATETHQTVYIPVVAPTPPSSL
                     TPQPVVLFNQTKAGRKLFTLDAEFPYCDVQTGKVIVVPRWYVTDFASVPWYGQGFIDP
                     QGPTARAAIVHDWLYAVGEPGKREEADAIFHRAMLKYGVSPFHANIAYQAVRAGGQKG
                     YG"
     gene            complement(3104..3556)
                     /locus_tag="PLH31_02900"
     CDS             complement(3104..3556)
                     /locus_tag="PLH31_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR88219.1"
                     /translation="MIQPHLSGGYILEELVVGMVAEKRVIVTEQRIQQFAEASDDFNP
                     VHMDEAFASRTAYRGRIAHGLLSASFGSAVVGTILPGAGAIYLGQTLAFHKPVRIGDE
                     VVSRVTVSAIDEASARVVLRCEAFVGEDLVMDGDATVRVPRRRRPARG"
     gene            3671..3910
                     /locus_tag="PLH31_02905"
     CDS             3671..3910
                     /locus_tag="PLH31_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88220.1"
                     /translation="MKDLNSASLADISAAGFDPVLAREVGFWRPYQTWDQLLLVGGVD
                     EAALERLQRRGFEIGSPNQDALTPPKPFRLSVSAR"
     gene            4073..>4701
                     /locus_tag="PLH31_02910"
     CDS             4073..>4701
                     /locus_tag="PLH31_02910"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR88221.1"
                     /translation="MTPFATDVSSKDLIDTLRSALVVLDEGLRVKSANRSFYALFQVQ
                     QAETEGRLIYDLGNRQWDIPALRELLERIIPRDEAIEGYEVDHHFEGIGRRTLLLNAR
                     KVYRPADHTMSLLLAIDDITAEREASRDSERAWRLAQTIVDTVRDPLVILEGDMTVAS
                     VNRAFLGLFGVTEGQVVGRQLKDLGEGQWDVSALGVLLAKVVPDEAAFDG"
BASE COUNT          743 a         1531 c         1650 g          767 t
ORIGIN      
        1 agctcatagg ttgattttgc cgcagaacgc cccctccgtc acgatgcgta ttcgcatcgc
       61 gccacctccc ccgcacgctt cgctacgggg caggagggta aagttgctct tctcctgccc
      121 cgcttgcggg ggaggtggcg cggcgcgaag cgacgtgacg gagggggcgt tctgagctga
      181 cagcctcagt accgatagag cttcacccgc tgcgtctgga acctcccggg ctccagtcgc
      241 acatgtcggc cctggtgggt ctcgtcgccg ttgagccagc ggccctcgac ccaggcgccg
      301 tcgacaaacc ggccttcggt caccttctcc agcccgacgc cttcgccgaa ggtcagggtg
      361 atccccgaac ccgcgaccag gaactcgtcg gcagagagcc ggatcaccag accgccatgc
      421 gcggtgaaga cctgcttgtc gcgcggcgtc cacggatcga cgaatgacac cttcacggtc
      481 cagtcgccca tcttcgcggt ctgcggcgtc tcatccacga cgccctcgaa gctcacgggc
      541 gggcggaagc cacgcgtcac gcccttgcct tgatgagcca ggagcacggg cgtcaactcg
      601 tccagcaggg cgtaggcgcc ggtcagcgga ttgccggccg gatcgctgat cgtctcgatg
      661 gcgaatggcg agaaaccgat cgcgtcgtgc tcgccgatcg cccagaaggc gttggctggg
      721 gcttccacct tgccggccct gtcggcctcg ggaaggaaaa ggggattatc cggccggtcg
      781 tactgcgccg cccagtgtgt gaagtcgggg tagtagatat ccggcgcctg gaacaccgac
      841 ggcgccgccg ccttccagac cgcgtacaga tgtggcaggg ggccggcgct gggatagccg
      901 cccggcgcaa cgcccggctt gttgagggcg gcattgacgt acatcggcag cgggtaggcg
      961 ctcgcccccg cgcgggccag gccctcgacg aaacggccat aggtccaggc ctgatacaat
     1021 tcgtccgtgg cggggctctt gccgaacacc tgttcccagt tgcccgagcc ggtcttgcct
     1081 gtggccttca gcacggcggc gggcacgggt ttcctgaact cggcctcggc cgccggcgag
     1141 tggtcgcggg ccgagggcag catgccgatc tcgttctcga cctggaccat gatcacggtg
     1201 tggcggtcgc catccaccgc cttcagatgc gccatcaggg cggcgaaggc ccgggcgtcg
     1261 gcggccaggg tggcgggcgc ggcgggcgac aggatttcct gggcctggcc ctgcgccgac
     1321 cgcgccagcg ggaagcgctg accgtcgcgc ttcacccagg ccggcgtata ggtcgacatg
     1381 ctgttcttcc aggccccgaa ccacagcagg accagccgca tgtcgctggc gcgggcctgc
     1441 gcgatcacgc catccacgac cgtgaagtcg aagcgcccct cgacaggctc gacctggtcc
     1501 cactgcacgg gagccagcac ggtgttgagg ttcagggcct tgagggttgg ccactgcttt
     1561 tgaagatagg gcaggctgga ggcggtggag ttgcccagct cgccgcccag gatcaggaac
     1621 ggcttgccgt cgaccagcag ctgggtcgcg tcgcccctgc gcgccaggcg ggggatctct
     1681 tcggcggccg cagcgggccc cagggcggtg gaggcgcaaa gcgcggcggt cagcgccagg
     1741 gcgcccaggg tctggatcag ggttcgcatc ggtggtcaga tgaccccatg cgacttgtaa
     1801 gatcaatccg gaaagcgctt gtccgcgtcc ctcaggccgc cgtctggatc gccatctggc
     1861 aggcccgcgc cagccgggtg ggcgagaccg gcttttccac cacggtgttg gtggcgcggc
     1921 tggagaggca cgaggcgttg ccggtgacat agaccaccgg gatggggcca agatgggcca
     1981 tgatcgccga gaccgcgtcc tcgccggtgc cgcctatgat gcgatagtcg gcggtgatca
     2041 ggtcgggctt gcggcgcagg gccagatcga gcgcctctcg tgcgttgtcg gccacatcga
     2101 ccgaggtgaa gccctgctct cgaagcaagg cttcgacttc cagagcaatc aggatctcgt
     2161 cttcgataat cagagcgtga cgcgggacca tctcatgcaa cctgttacaa atatccccac
     2221 gcctccacac agccgactga ttccaagcaa aggcaagggc cacgtgattt cgccggcgcg
     2281 acagggctgc ggcgtaaggt caccggcggg cgctatttct tgcggtcggc gccccagggc
     2341 agcttctgct tgacctggtc cagcggtgtc ctggtgacca ccacgggggg cggcacgcgc
     2401 accgtggggg ccttgatcgg ataggcgcgc cagccgctct ggatcagggc cccaaagccc
     2461 tggcagcgcg gcaggatccg cacggcgccg gnnnnnnnnn ngccatagcc cttttgcccg
     2521 ccggcccgca cggcctggta ggcgatattg gcgtggaagg gcgagacgcc gtatttcagc
     2581 atcgcgcggt ggaagatggc gtcagcctcc tcgcgcttgc ccggctcgcc cacggcgtac
     2641 agccagtcat ggacgatggc ggcgcgggcg gtggggccct gggggtcgat gaagccctgg
     2701 ccgtaccagg gcacgctggc gaagtcggtg acgtaccagc gggggacgac gatgaccttg
     2761 ccggtctgga cgtcgcaata ggggaactcg gcgtccaggg tgaacagctt gcggccggcc
     2821 ttggtctggt tgaacagcac caccggctgc ggcgtcagcg agctgggcgg ggtgggcgcg
     2881 accacgggga tgtagacggt ctggtgcgtc tccgtcgcgc agctggcgag gatcgaggcg
     2941 cccagcaagg cggcggcggc gagaagggcg gttctgatcg ggcgcaaggc ggtcttcgga
     3001 gcgtgcggag gcgagatcgg cctatagcac cgtttccgtg gcgctttcga ggcggcatga
     3061 gcccgcggcg accaggcccc cgcgattgct caggcggcgg gcttcagccc ctggcgggcc
     3121 gccgccgacg cggcacgcga acggtggcgt cgccgtccat gaccagatcc tcgccgacga
     3181 aggcctcgca gcgcagaacc acgcgggcgc tggcctcgtc tatggccgag acggtgacgc
     3241 gggagacgac ttcgtcgccg atccgcaccg gtttgtggaa ggccagggtc tgacccagat
     3301 agatcgcgcc cgccccgggc aggatggtgc cgaccacggc cgagccgaac gaggccgaca
     3361 gcaggccatg ggcgatgcgg ccgcgatagg cggtgcgcga ggcgaaggcc tcgtccatgt
     3421 gcacgggatt gaagtcgtcc gaggcctcgg caaactgctg gatgcgctgc tcggtgacga
     3481 tgacgcgctt ttcggcgacc atcccgacca cgagctcttc cagaatatag ccgccggaaa
     3541 gatgaggttg gatcataccg ggcggttagc gatgatgggg cgtcgtggct agtggaaatg
     3601 ctccatgccg gccgccagtc tttttcctgg acggcatgga accttggccg tggacgcgcg
     3661 ctgtaagtcg atgaaggatc tgaacagcgc cagcctggcc gatatctctg cggcgggttt
     3721 cgatcccgta ctggcgcgcg aggtcgggtt ctggcgcccc tatcaaacct gggatcaact
     3781 gctgctggtg ggcggcgtcg atgaggccgc gctggagcgg ctccagcgcc ggggcttcga
     3841 gatcggctcg ccgaaccagg acgccctgac gccgcccaag cctttcaggc tttcggtcag
     3901 cgcgcgctga cggctcgggt cgaagcctcc gtcgggggcg gccaccgccg ccttgaacga
     3961 tcttcagcct gcccgacgcc gcctgccggt gtcggggccg atcccgaccc tctgagggtc
     4021 gaaacctctc ctgcgcctcc tgatgggcag cgcggcgcca aaaggaccgc gcatgacccc
     4081 ctttgccacc gacgtttcgt cgaaagatct gatcgacacc cttcgcagcg ctcttgtggt
     4141 gctggacgag ggcctgcgcg taaagtccgc caaccggtca ttctacgccc tgttccaggt
     4201 gcagcaggcc gagaccgaag gccgcctgat ctacgatctg ggcaaccgcc agtgggacat
     4261 ccccgccctg cgcgagctgc tggagcggat catcccgcgc gatgaggcga tcgagggcta
     4321 tgaggtcgat catcatttcg aggggatcgg tcgacgcacg ctgctgctga acgcccgcaa
     4381 ggtctatcgg ccggctgatc acaccatgtc gctgttgctg gccatcgacg atatcaccgc
     4441 cgagcgggaa gcctcgcgcg acagcgagcg cgcctggcgc ctggcccaga cgatcgtcga
     4501 cacggtgcgc gaccctctgg tgatcctgga gggcgacatg accgtcgcca gcgtcaatcg
     4561 cgcctttctg ggtctgtttg gggtcaccga aggccaggtc gtggggcgcc agctgaagga
     4621 cctgggcgag gggcaatggg atgtctcggc cctgggcgtg ctgctggcca aggtggtgcc
     4681 tgacgaggcc gccttcgacg g
//
LOCUS       DAOMEO010000083         4694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2261_length_4694_cov_2.606596, whole genome shotgun sequence.
ACCESSION   DAOMEO010000083 DAOMEO010000000
VERSION     DAOMEO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_4694_cov_2.606596"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..379)
                     /locus_tag="PLH31_02915"
     CDS             complement(8..379)
                     /locus_tag="PLH31_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR88222.1"
                     /translation="MILVDTSVWIDHFRAASPDLVRLLEQGRVLVHPFVVGELALGNL
                     RDRTTVLGALKQMPQAVRAHDDEVLSFIEANRLHGLGVGYIDAHLLASARLTTGARFW
                     TRDRRLNAVSEQLSVAFHPQG"
     gene            complement(376..573)
                     /locus_tag="PLH31_02920"
     CDS             complement(376..573)
                     /locus_tag="PLH31_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQR88223.1"
                     /translation="MRTTLALDDELLARAIDLTGIEEKTALVREALNALVERESARRL
                     ARLGGSQPDLKAAPRRRPQSA"
     gene            complement(638..1348)
                     /locus_tag="PLH31_02925"
     CDS             complement(638..1348)
                     /locus_tag="PLH31_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HQR88224.1"
                     /translation="MTDEEPPRKRMVKPPAGGTDAGRTKPARLKKTYGRTTSQQAWLE
                     RQINDPFSAKARAMGYRSRAAFKISEIDEKFGFFKKGVRVIDLGCAPGGWLQIAVERG
                     VTQLVGVDLLPVDPVEPAHILEMDFTDDACPPKLIELLGGAPDVVLSDMAPNTVGHRE
                     TDHLRIVGLIEMGAEFAIEVLKPGGAFVAKAFQGGETADVIARLKKNFDKVVHYKPKA
                     SRADSSEVFLVATGFRGR"
     gene            complement(1345..2459)
                     /locus_tag="PLH31_02930"
                     /pseudo
     CDS             complement(1345..2459)
                     /locus_tag="PLH31_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
     gene            2614..2943
                     /gene="hspQ"
                     /locus_tag="PLH31_02935"
     CDS             2614..2943
                     /gene="hspQ"
                     /locus_tag="PLH31_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HQR88225.1"
                     /translation="MNSRFAKFAIGQVVKHRIFPFRGVVFDVDPVFANTDEWWESIPE
                     DIRPIKDQPFYHLLAENDENTYVAYVSEQNLLADETGEPVQHPQTAVIFESFDHGAYK
                     LRPRISH"
     gene            3025..3906
                     /gene="phhA"
                     /locus_tag="PLH31_02940"
     CDS             3025..3906
                     /gene="phhA"
                     /locus_tag="PLH31_02940"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HQR88226.1"
                     /translation="MSADGFTTSPPPGARADWTIDQGWESYSRAEHDVWITLYERQTA
                     MLPGRACDAFLRGLDALDLHRSGIPDFRRINEELARLTGWSVVAVPGLVPDDVFFDHL
                     ANRRFPAGQFIRKPDELDYLQEPDIFHDVFGHVPMLTDPVFADYMQAYGQGGQRALEL
                     GRLANLARLYWYTVEFGLMQTPQGLRIYGAGIVSSRAESIFALDDPSPNRIGFDLERV
                     MRTPYRIDDFQQAYFVIDSIQTLQAVTLQDFAPIYDRLEGASDIGIAEILPGDTVFTR
                     GTQAYAGAGGRLASARG"
     gene            complement(3864..>4694)
                     /locus_tag="PLH31_02945"
     CDS             complement(3864..>4694)
                     /locus_tag="PLH31_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQR88227.1"
                     /translation="GFCLKYATASGHWIGPDEGGRLITPTWNHMWFVAYLLVYSVILA
                     MMLAFMPRLGATLQAGLERTLKGPGLLIWPVVFLAATRMFLMPRFEITHALVDDWYNH
                     AVSFSAFLLGYGLAKSEIVRERLVGARWMTLILAVVCWAGWATYAWLYRADDANPPEA
                     LRLVMRGVYATDQWCAIAAILGFGARHLNRDAPALRYLTLAVFPFYIVHQTLIVVLAY
                     HLARLNLPQGLEAALLIGATFAGCFMTYEIVRRIPVVCWLFGLKSQPRAEASRPPAPA
                     "
BASE COUNT          853 a         1590 c         1468 g          783 t
ORIGIN      
        1 aattcgttca cccctgggga tgaaacgcga cagacagttg ctcactgacg gcgtttagcc
       61 gccgatcccg tgtccagaac ctagcgcctg tcgtcagccg ggcgctggca agcagatggg
      121 cgtcaatata accaacgcca aggccatgga gacgattggc ttctatgaag ctcagcacct
      181 cgtcgtcatg agcgcggaca gcctgcggca tttgcttcaa ggcgcccaga accgttgtac
      241 ggtctcgcag atttcccagc gcgagctctc cgaccacaaa tgggtgaacc agcacccgcc
      301 cctgttccag cagacgcacc aggtcgggac tggccgcacg gaaatgatcg atccagaccg
      361 aggtatcaac caggatcacg cggactgggg cctacggcga ggagccgcct tcagatctgg
      421 ctggctaccg cctagtcgcg ccagcctgcg tgcgctttca cgttcgacca gggcgttgag
      481 cgcctcacga accagcgccg tcttctcttc gatcccggtc agatcgatcg cccgagccag
      541 cagctcgtca tcgagcgcca atgtggtgcg cattgtgcaa cctctagtta ggcacggatc
      601 atgacatcaa atgatgccaa aatccatgca agcgtgttca ccgcccccgg aaccccgtcg
      661 ccaccaggaa cacctcggag ctgtccgccc ggctggcctt gggcttgtag tgcacgacct
      721 tgtcgaagtt cttcttcagg cgggcgatga cgtcagccgt ctcgccgccc tggaaggcct
      781 tggccacgaa ggcgccgccg ggcttgagca cctcgatggc gaactcggcg cccatctcga
      841 tcaggccgac gatgcgcagg tggtcggttt cgcggtggcc gacggtattg ggcgccatgt
      901 ccgacagcac cacgtccggc gcgccgccca gcagctcgat cagcttgggc ggacaggcgt
      961 cgtcggtgaa gtccatctcc aggatgtggg cgggctcgac gggatcgacc ggcagcaggt
     1021 cgacgccgac cagttgggtc acgccgcgct cgaccgcgat ctgcagccag ccgccgggag
     1081 cgcagcccag gtcgatgacg cgaacgccct tcttgaagaa gccgaacttc tcgtcgatct
     1141 cgctgatctt gaaggccgcg cggctgcgat agcccatggc gcgggccttg gccgagaacg
     1201 ggtcgttgat ctgccgctcc agccacgcct gctgcgaggt ggtgcggcca taggtctttt
     1261 tcagccgcgc gggcttggtg cggcccgcgt cagtgccacc ggccggcggc ttgaccatgc
     1321 gcttgcgggg cggttcttcg tcggtcattc ggacaccggt ttggacttgg acgcgccgcc
     1381 acggcgtcgc cgacgcgggc ggcgctgctg ttcggacatc agggacatca gaagtccctc
     1441 tctaagccct cgatcggcca cacgcacgcg cgaacacggc caaagctcct gaacagcctg
     1501 aagaatcgcc gcgccggcta ggaccagatc cgcacgatcg gccccgatac acggctccag
     1561 cgcccgttcg gcggcggtca ggcccagcag gcggtcagcg gcggcttcgc aatccttgcg
     1621 ctccatccac agcccatcga ccacattgcg atcatagcgt tggaggccca ggtgcaggcc
     1681 ggccaggctg gtgatcgcgc ccgaggtgcc gaccagatgc gcccggtcct gttcaaatag
     1741 ctcccgcatc ggctcggcat ggggaaaggc ctcgatccgc gccttgacgt cgtcgaccat
     1801 tgcccggaac cagcccgcct cggcccgctc gccctcggga aagcgttcgg ccagggtgac
     1861 gactcccacc gggatcgaca gccaggccct gatcggcagc ttccaggcgg cgaactgccg
     1921 cggcttggcg tccagccccc cgcccttcag gtcgacccag gacaactcgg tcgaaccgcc
     1981 ccccacatcc accaccagcg cagcgtcggc gttgcgatca aacaggctca tgcagccggc
     2041 cacggagagc tgggcttctt cacgcggcgt gatgatctgc aggcgcagcc ccgtctcttc
     2101 ggccacgcgt tgcacgaagg ccgcgccatt gtcggcgccg cgacaggcct gggtggcgac
     2161 ggccttgatc ttgatggcct tgcggcggcg gatcttctcg gcgcagacct tcaaggccgc
     2221 catggaacga tccatggccg catcggacag catgcccgtc tgcgagaggc cttcgcccag
     2281 acgtacgatg cgagaatagg cctcgacgac gcggaacccg cgcggagacg gcgtcgccac
     2341 caggaggcga caattattgg tgcccagatc gagcgccgct cgctgggcgg ccgccggcgc
     2401 cgcccgatct cctcacgagg agaaattggg acgccgggcg cacgcagcgc ctccgacatg
     2461 gtctttccac ctgtctcgca gacgccgcaa atcacgacgc ctcacttcga gacagagcat
     2521 agcaacgcga cggcgacggg cggaagcctt gttatccgtc tgcggaattc tacgttcagg
     2581 attcagtcag gtgagcctgt tatctttaac cgcatgaatt cgagattcgc caaattcgcg
     2641 atcggccagg tggtcaagca ccggatcttc ccgtttcggg gggtggtgtt cgacgtcgac
     2701 cccgtgttcg ccaacaccga cgagtggtgg gaatcgatcc cggaggacat ccggccgatc
     2761 aaggaccagc cgttctatca cctgctggcc gagaacgacg aaaacaccta tgtcgcctat
     2821 gtgtcggagc agaacctgct ggctgacgag accggcgagc cggtgcagca tccgcaaacg
     2881 gcggtgatct tcgagtcctt cgatcacggc gcctacaagc ttcgaccccg gatttcgcac
     2941 taggcgcacg gcctctccag aaaacaccgg tttaagggta aattccggac gatatttgcg
     3001 ccgaacgggg cgtagcgtcg ttgcatgagc gctgacggtt ttaccacctc gccaccgccc
     3061 ggagcccggg cggactggac catagatcag ggctgggaaa gctattcccg ggccgaacac
     3121 gacgtctgga tcacgctcta tgagcgccag accgccatgc tgccgggccg cgcctgcgac
     3181 gccttcctgc gcggtctcga cgccctggac ctgcatcgct ccggcatccc cgatttcagg
     3241 cggatcaacg aagagcttgc gcgcctgacc ggctggagcg tggtcgccgt gccgggcctg
     3301 gtgcccgacg acgtgttctt cgaccatctg gccaaccgcc gcttccccgc gggccagttc
     3361 atccgcaagc ccgatgagtt ggactacctg caggaacccg acatcttcca cgacgtcttc
     3421 ggccacgtgc cgatgctgac tgacccggtc ttcgccgact acatgcaggc ctatggtcag
     3481 ggcgggcagc gggcgctgga gctgggccgc ctggccaatc tcgcccgcct ctactggtac
     3541 acggtcgagt ttggcctgat gcagacgcct cagggcctgc ggatctacgg cgccggcatc
     3601 gtctcgtcgc gggccgagtc gatcttcgcc ctggacgatc cctcccccaa ccgcatcggt
     3661 ttcgacctgg agcgggtgat gcgcacgccc taccggatcg acgacttcca gcaggcctat
     3721 ttcgtcatcg actcgattca gaccctgcag gcggtcacac tgcaggactt cgcgccgatc
     3781 tatgatcgtc tggagggcgc cagcgacatc ggcatcgccg agatcctgcc cggcgacacc
     3841 gtgttcacgc gcggaaccca ggcctacgcc ggcgccggcg gccggctggc ctcggcgcgc
     3901 ggctgagact tcaggccaaa cagccagcac accaccggga tgcgtcgcac gatctcgtag
     3961 gtcatgaaac agccagcgaa ggtcgcgccg atcaggaggg ccgcctccag gccttgcggc
     4021 aggttcagcc tggccaggtg ataggccagg accacgatca gggtctggtg gacgatgtag
     4081 aacggaaaca ccgccagggt caggtagcgc agggccggcg catcgcggtt cagatgtcgg
     4141 gcgccaaagc ccaggatggc ggcgatcgcg caccactgat ctgtcgcata gacgccgcgc
     4201 atcacgaggc gcagcgcttc aggcggattg gcgtcgtcgg cgcgatagag ccaggcatag
     4261 gtcgcccagc cggcccagca gacgacggcc aggatcaggg tcatccagcg tgcgccaacc
     4321 agtcgctcgc gcacaatctc cgacttggcg aggccgtagc ccagcaggaa cgcggaaaac
     4381 gagacggcgt ggttatacca gtcatccact agggcgtggg tgatctcgaa ccgcggcatc
     4441 aggaacattc gagtcgctgc cagaaagacg accggccaga tcagcaggcc aggtcccttc
     4501 agcgtacgct ccagtcccgc ctgcagcgtc gcgcccagcc tgggcatgaa cgccagcatc
     4561 atggccagga tcaccgaata gaccagcaga taggccacga accacatgtg gttccaggtc
     4621 ggcgttatca ggcgtcctcc ttcgtcgggc ccgatccagt ggcccgatgc ggtggcgtat
     4681 ttcagacaga agcc
//
LOCUS       DAOMEO010000084         4679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2276_length_4679_cov_2.722967, whole genome shotgun sequence.
ACCESSION   DAOMEO010000084 DAOMEO010000000
VERSION     DAOMEO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2276_length_4679_cov_2.722967"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1972
                     /locus_tag="PLH31_02950"
                     /pseudo
     CDS             47..1972
                     /locus_tag="PLH31_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515713.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2056..3372
                     /locus_tag="PLH31_02955"
     CDS             2056..3372
                     /locus_tag="PLH31_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88228.1"
                     /translation="MSQSQSQIEPSAPGSRYRYVVLAMLILAYTFNFLDRQILGILAG
                     SIKADLNLTDTQLGLMGGVAFAALYTTLGVPLAWLADRVSRTWIMTVALTVWSGFTVA
                     CGFAGGFWSLFLSRMGVGVGEAGGVAPAYSLIADYFPKEQRARALAVYSFGIPLGTAL
                     GVLFGGLIAAYVNWRVAFIVVGLAGVALAPIFKWVVKDPPRGGMDRAPQTLASGEAAP
                     LIAPKAPAFGKVLATILPKPSFWLLSFGAACSSICGYGVAFWLPTFFQRSFGLSLTDR
                     ALFYSALSLIGGVAGIWLGGVLADRFGAKNKAAYALAPAICFLVALPCFLLAMNVNSM
                     VWAFLIFLIPTGLNLAWLGPVVAAVQHLAPASMRTTTSALFLLINNLLGLAVGLWFFG
                     YVSDLLAPRYGAESMRHAIYIGLGFYVISAVLLIFASRRLKRDWVD"
     gene            3581..>4679
                     /gene="sucC"
                     /locus_tag="PLH31_02960"
     CDS             3581..>4679
                     /gene="sucC"
                     /locus_tag="PLH31_02960"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQR88229.1"
                     /translation="MNIHEHQAKAVLAEFGAPVPRGFAVFTPEEAFEAAQKLGTPVFV
                     VKSQIHAGGRGKGTFEGLGPDAKGGVRVVKTAEDVKTNAAEMLGRVLVTHQTGPKGKQ
                     VNRLYIEEGAAIAKEFYLSLLVDRETSMVSVVASTEGGMDIEDVARATPEKIHSFSID
                     PATGVWPTHQRALAKALGLTGGLAKEAAVLLTQLYTAFLAKDMAMLEINPLIVTADDH
                     LKVLDAKLSFDGNALYRHPDIKALRDETEEDPKEIEASKYDLAYIALDGEIGCMVNGA
                     GLAMATMDIIKLYGAEPANFLDVGGGANKEKVTAAFKIITADPAVKGILVNIFGGIMR
                     CDIIAEGVIAAVKEVGLQVPLVVRLEGTNVEL"
BASE COUNT          784 a         1617 c         1461 g          817 t
ORIGIN      
        1 gcattagggg cgggtccgac ctcatttctt tccttgcatt gagttcatct tttggtatga
       61 gcccggcgtt ggcctgtatg tcgacgatgt ctatatctat cgcccgcagg gcgcggtgct
      121 ggacgtgttc gacgtcgagc gcatcgaagt gctgcgcggc ccgcagggca cgctgtacgg
      181 ccgcaacacc atcggcggcg ccatcaagta cgtgaccaag cgcctgggcg acgaggccca
      241 tggcaaggtg cgcggcacct atggcagcta caaccagacc gacctgctgg tctcgggcca
      301 gacgccgatc ggcggcacgg gcctgtcggt gggcggcgcc tatgcgctct acaagcgtga
      361 cggctacggc aagaacctga ccaccggcgc cgagcactac aacaaggacg tccaggccat
      421 ccgcctcagc gccgaatatg cgcccaatga cgacctgttc ttccgcctgg cctatgacca
      481 cgtgcaggac gattccaacc cccgtcacgg ccaccgcgaa ctgccggccc tggtcggcgg
      541 ctacaaggtt ctggacgagt acgacaccca ggccggcttt ggcgacgaca actcggtgga
      601 aaccgaaggc gtctcgctga ccgcccagtg gaacgcctcc gaggccctga ccctcaagtc
      661 gatcaccgcc agccgcaagg gtcacaccga cacggtcatc gacttcgacg gcacgccgct
      721 gtcgacgctg gacgtgcccg cctactacga cgaccgctcg ttctcgcagg aactgcaggc
      781 cgtctattcg gacgacaacg tccagggcgt gtttggcctg tactacatga acagccaggc
      841 ctcgggcgag ttcgacacca tcgccggtaa cctgggtctg tccatcgccg acggcggcaa
      901 ggtcagcacc cagagcttcg cggccttctt cgacttcagc gccaacctga ccgacaagtt
      961 caaggtctcg ctgggcgccc gcgccacgcg cgatcacaag cgcagcacga ccttccgcct
     1021 gttctatctg ggcgccacgc gctcgcccta tcagggcggc acgccgcgtc cgatcctgca
     1081 gacccgcacc aactacggcg cccagaccac cttctcgcag ttcacgccgc gccttagcgc
     1141 ctcgtatgag ctgaccgacg acctgagcac ctatgtctcc tattccaagg gcttcaagtc
     1201 cggcggctgg gacatgcgcg gcgacgccct gatcaccccg cagaccgtca acggctacaa
     1261 gcccgaggtg gtcgacgcct atgaagtggg cctgaagggc tatgccttcg atcgccgggt
     1321 ctcgttcgcc tcggcggcct tcctgtcgaa gtacgaagac cagcaggtct cgacccaggt
     1381 ggtggtgccc agcggcatcg ccagctcgat cgacaacgct ggctcgtcca ccctgtatgg
     1441 cgtggaattc gaagccaagg ccaggctgac cgccaacctg tcggccaatg tcgccgtcgg
     1501 ctacatccac gccgagtacg acaccttcaa ccgcttcgtg ccggccggcg cgcccaaccc
     1561 gctgaacccg tcgacggtta tcccggccgg cggccaggtg gtgaacgtgg ccgacttgta
     1621 cggcttccag aacaccccgg aatggaccgg caatgtcagc ctgacctggg tgggcgaact
     1681 ggccggcggc gtgctggccg tcaccccgat ggtcagctat cgcgacagct atcagcagtt
     1741 cgagcagcct cttccgctgc tggaccagaa ggccttcacc ctggtggacc tgacggcgat
     1801 ctggacggcg ccgggcggtc actacaagct ggcgctgacc ggcaagaacc tgaccgacga
     1861 acgttatcgc acgggcggct ataatttcgg cgtgccgacc tataacaact cggtcatcgg
     1921 tttctacggc ccgccgcgca ccgtcgcggc cagcctcgaa atcgcattct gatctaggta
     1981 aaaactggcg gcggcccgct gcaacgggcc gccgccattt ctttgtctgc agcgtaagaa
     2041 tacagcgggg ggaccatgag ccagagccag agccagatcg agccatcggc gcccgggtcc
     2101 cgctatcgct acgtggtgct ggcgatgctg atcctcgcct acaccttcaa tttcctggac
     2161 cggcagatcc tcggcatcct ggccgggtcg atcaaggccg acctcaatct caccgacacc
     2221 cagctgggcc tgatgggcgg ggtcgccttc gcggcgctct acaccaccct gggcgtgccg
     2281 ctggcctggc tggccgaccg ggtcagccgc acctggatca tgaccgtcgc cctgaccgtc
     2341 tggagcggct tcacggtggc gtgcggcttt gccggcgggt tctggagcct gttcctgtcg
     2401 cgcatgggcg tgggtgtcgg cgaggcgggc ggcgtggcgc cggcctattc gctgatcgcc
     2461 gactatttcc ccaaggagca gcgggcccgg gccctggcgg tctattcgtt cggcattccg
     2521 ctgggcacgg ccctgggcgt gctgtttggc ggcctgatcg cggcttacgt caactggcgg
     2581 gtggccttca tcgtggtggg cctggctggc gtggccctgg ccccgatctt caagtgggtg
     2641 gtcaaggatc cgccgcgcgg cggcatggat cgcgcgcccc aaaccctcgc ttcaggcgag
     2701 gcggcgccgc tgatcgcccc caaggccccg gccttcggca aggtgctggc caccatcctg
     2761 cccaagccca gcttctggct tctgtcgttc ggcgcggcct gctcgtcgat ctgcggctat
     2821 ggcgtggcct tctggctgcc gaccttcttc cagcgcagct tcgggctgag cctcaccgac
     2881 cgcgccctgt tctacagcgc cctgtcgctg atcggcggcg tggccggcat ctggctgggc
     2941 ggcgtgctgg ccgaccggtt cggcgccaag aacaaggccg cctacgccct ggccccggct
     3001 atctgcttcc tggtggccct gccgtgtttc ctgctggcca tgaacgtcaa ttcgatggtc
     3061 tgggccttcc tgatctttct gatccccacc ggcctgaacc tggcgtggct gggaccggtg
     3121 gtcgcggcgg tgcagcacct ggccccggcc tcgatgcgga ccaccacctc ggccctgttc
     3181 ctgctgatca acaacctgct gggcctggcc gtgggcctgt ggttcttcgg ctatgtctcc
     3241 gacctgctcg cgccccgtta cggagcggag tcgatgcgcc atgcgatcta tatcggcctg
     3301 ggcttctatg tgatcagcgc cgtgctgctg atcttcgcct cgcgccggct gaagcgcgac
     3361 tgggtcgact gacccggccg cggggtatgg gaccggtaac agttccgcaa ggtcaggggc
     3421 ttccttgccc tgggcgctca cgagtgcggc gccgctgtcc tttcggacat aagaagcgcg
     3481 caaggggcga ccttatcgcc caagcgcggt tgcgcctgcg aaatcacgct cctataaccc
     3541 ggccacattt cagaaaagcc gtcgtcgaga ccgggccctc atgaacattc acgaacacca
     3601 agccaaagcc gtactcgcag agttcggcgc ccctgttccg cgcggctttg ccgtgttcac
     3661 ccccgaagaa gccttcgagg ccgcccagaa gctgggcacg ccggtcttcg tggtgaaaag
     3721 ccagatccac gccggtggcc gtggcaaggg cacgttcgag ggcctgggcc ccgacgccaa
     3781 gggcggcgtt cgcgtcgtca agaccgccga ggacgtcaag accaacgccg ccgagatgct
     3841 cggccgcgtg ctggtgacgc accagaccgg ccccaagggc aagcaggtca accgcctcta
     3901 catcgaggaa ggcgcggcga tcgccaagga attctacctg tcgctgctgg ttgatcgcga
     3961 aacctcgatg gtttcggtgg tcgcctcgac cgaaggcggc atggacatcg aggacgtggc
     4021 ccgcgccacg cccgagaaga tccacagctt ctcgatcgac cccgccaccg gcgtgtggcc
     4081 gacccaccag cgcgcgctgg ccaaggccct gggcctgacc ggcggcctgg ccaaggaagc
     4141 cgccgtgctg ctgacgcagc tctatacggc gttcctggcc aaggacatgg ccatgctgga
     4201 gatcaacccg ctgatcgtca ccgccgatga tcacctgaag gtgctggacg ccaagctgtc
     4261 gttcgacggc aacgccctct atcgccaccc ggacatcaag gccctgcgcg acgagaccga
     4321 ggaagacccc aaggaaatcg aagccagcaa gtatgacctg gcctatatcg ccctcgacgg
     4381 cgaaatcggc tgcatggtca acggcgcggg cctggccatg gccaccatgg acatcatcaa
     4441 gctgtacggc gccgagccgg ccaacttcct cgatgtcggc ggcggcgcca acaaggagaa
     4501 ggtcaccgcg gccttcaaga tcatcaccgc tgaccccgcc gtgaagggca ttctggtcaa
     4561 catcttcggc ggcatcatgc gctgcgacat catcgcggaa ggcgtcatcg ccgccgtgaa
     4621 ggaagtcggg ctgcaggttc cgctggtggt gcgcctggaa ggcaccaacg tcgaactcg
//
LOCUS       DAOMEO010000085         4678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2278_length_4678_cov_2.398875, whole genome shotgun sequence.
ACCESSION   DAOMEO010000085 DAOMEO010000000
VERSION     DAOMEO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_4678_cov_2.398875"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLH31_02965"
     CDS             complement(<1..732)
                     /locus_tag="PLH31_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR88230.1"
                     /translation="MVLSRPPPGGLGGAFDPTLQLLHQAVAAFNAGLWAEAERFARQV
                     LAVRKNDPSALNVLGGAAMNTGRSEAAVPLFEAAARGQPRNPFIAFNLGEAHRRAGRF
                     REAAGALRRAIQLKPDFAEAHAYLGEAYRALGRSDEAEAAYRRALAIQTQLPTALMGL
                     GALARKAGDLELAARFLGGARDALAGQPSRARACSQLGELLLDLGQRPMALAALDEAV
                     RLAPEVPGYWRQLAHGLRDSKVAPEG"
     gene            complement(754..1632)
                     /locus_tag="PLH31_02970"
     CDS             complement(754..1632)
                     /locus_tag="PLH31_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQR88231.1"
                     /translation="MTQRPAGERAQYRVTGMDCGDCAAKIEAGVRKLEGVDDVRVSIA
                     SELMTLHVDDRERRLPEVERTVQGLGYHLERIDAAAPMGSGEAKDTVRSDPGYRRALW
                     IVVLLNVGYGLVEIGAGLFGRSQALQADALDFFGDGLITFLGLLAIGWSLAWRARAAL
                     IQGIFLAVLGLGVLVSTTYRVLVLNEPNAEIMGVFGAIALVVNVIAAIVLIPHRAGDA
                     NARAVWLFSRNDALGNLAVVIAAGLVFWMRTPWPDLVVAFVIAGLFLQSASSIIRHAR
                     ADLRDAGADPLPAPRP"
     gene            complement(1722..2264)
                     /locus_tag="PLH31_02975"
     CDS             complement(1722..2264)
                     /locus_tag="PLH31_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR88232.1"
                     /translation="MPLSPDQMGIVRGSALALAIAGATLAAAWAWLPAQSMGVAPGFD
                     AGERLVYALKTALPVFLWLAIAIRGVSGRRFTSPADIRGSAFAPPSPELKVPVAILQN
                     SLEQTVLFMGAHLILAAVLRDGELVLLPTMVLLYMAGRVCFAVGYARGASGRAFGMAL
                     TAGPTIFGLVTAGVLMGLGR"
     gene            2413..4341
                     /locus_tag="PLH31_02980"
     CDS             2413..4341
                     /locus_tag="PLH31_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HQR88233.1"
                     /translation="MPAALRLFAFVLLLTATAAPLRALAQEAPPAQVAWRLLDYISVD
                     YPGAVQGDRVISAAEYAEMQEFAKTVRVRLASLPATPAKAGLLQGADALATEIAHKAP
                     PAEVAVLARRLAGELLAAYPTPLAPATTPDLALGARLYAEQCAACHGATGHADGPNAK
                     GLDPAPVAFADPTRARERSLFGLYQVIGQGLDGTAMASFAHLSDTDRWALAFYAGGFA
                     FDADAAQAGEALWKADPNLRARFTNLQAVTQVTPAALAAELGEPRATQLTAFLRRQPS
                     AVTPSGEGSLDVARTKLQQSLAAYQAGDPKRAENLALPAYLDGFEPVEPTLKARDGAL
                     MVRIETAMGELRASVSSRAPAADVATKVERLIALLNVAEQALAPERASAASSFAGAFA
                     ILLREGLEALLIVVAMIAFLGKADRRDVLPYVHAGWISALAAGAATWAAATWLISISG
                     ASRELMEGFGSLVSAAVLVSVGVWMHGKSHAEAWQRYIKEKLSAALSKGSAWFLFGLA
                     FVVVYREVFETILFFAALWAQGGHLALMAGAGVAMVFLAVIARLLLTYSRRLPITQFF
                     AYSALLIAVLAVVLAGKGVAGLQEAGLLDVHPLAGAPRIEMLGVYPTWEGLWAQLATL
                     ALLVVGFRIAGRKTATAA"
     gene            complement(4338..>4678)
                     /gene="nadE"
                     /locus_tag="PLH31_02985"
     CDS             complement(4338..>4678)
                     /gene="nadE"
                     /locus_tag="PLH31_02985"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522484.1"
                     /note="catalyzes the formation of nicotinamide adenine
                     dinucleotide (NAD) from nicotinic acid adenine
                     dinucleotide (NAAD) using either ammonia or glutamine as
                     the amide donor and ATP; ammonia-utilizing enzymes include
                     the ones from Bacillus and Escherichia coli while
                     glutamine-utilizing enzymes include the Mycobacterial one;
                     forms homodimers; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQR88234.1"
                     /translation="LQDFNLFHITRFGLRPSKVAFLAWHAWRDVAEGAWPENFPEETR
                     NAYDLATIKKWLGVFLFRFFEISQFKRSALPNGPKVISGGALSPRGDWRAPSDGTAKI
                     WLDELEANVP"
BASE COUNT          736 a         1646 c         1594 g          702 t
ORIGIN      
        1 gccttccggt gcgaccttgc tgtcgcgcaa gccgtgcgcc agctggcgcc aatagcccgg
       61 cacctcgggc gctagccgga ccgcctcgtc caacgccgcg agcgccatgg gccgctggcc
      121 caggtcgagc agtaactcgc cgagctgcga acaggccctc gcgcggcttg gttgtcccgc
      181 gagcgcgtcg cgtgcgccgc cgaggaaacg tgccgccaat tccaggtcgc ccgccttccg
      241 cgccaaggcg ccaaggccca tcagggccgt tggcaactgg gtctggattg ccagggcgcg
      301 gcggtaggcc gcctcggcct cgtcagagcg accgagtgcg cggtaggcct ccccgagata
      361 ggcgtgagcc tccgcgaagt cgggtttcag ctggatggcg cggcgcaacg cccctgcagc
      421 ctcccggaaa cggcctgctc tgcgatgcgc ctcaccaaga ttgaaggcga tgaacggatt
      481 gcggggctgg ccgcgggccg ccgcttcgaa gagggggacc gccgcctcag agcggccggt
      541 gttcatggcg gccccaccga ggacattgag agccgagggg tcattcttgc ggacggccag
      601 gacttggcgg gcgaatcgct cggcctccgc ccagagcccg gcgttgaagg cggcgacggc
      661 ttggtggagc agttggagcg tgggatcgaa cgctccgcca aggcccccgg gcggcggacg
      721 gctcaacacc atgagagcga ccgcctcacc aagtcacggt cgcggcgcgg gcagcggatc
      781 ggcgccagcg tcccgaaggt cggcgcgggc gtgacggatg atcgacgagg cggactgtag
      841 gaacaggccg gcgatgacga aggccacgac cagatcgggc catggtgtcc gcatccagaa
      901 caccaggccc gccgcgatca ccactgccag gttccccagc gcatcgttgc gggagaacag
      961 ccagaccgcc cgcgcgttgg cgtcgccggc gcggtgcggg atcagcacga tggcggcgat
     1021 cacgttgacg accagggcga tcgcgccgaa cacgcccatg atctcggcgt tgggttcgtt
     1081 gaggaccagg acgcggtacg tcgtgctgac caggacgccc aggcccagca cggccaggaa
     1141 tatcccctgg atcagggcgg cgcgagcgcg ccacgccaga ctccagccga tcgccaacaa
     1201 cccaaggaag gtgatcagcc cgtcgccgaa gaagtcgagg gcgtcggcct gcagggcctg
     1261 ggatcggccg aacaggcccg cgccaatctc caccaggccg tagccgacgt tgagcagaac
     1321 gacgatccac agcgcgcggc ggtagcccgg gtcactgcgc acggtgtcct tcgcctcgcc
     1381 ggaacccatc ggcgcagcag cgtcgatccg ctcgaggtga tagccaaggc cttgcacagt
     1441 acgctcgacc tcgggaagcc ggcgttcgcg gtcgtcgacg tgcagggtca tcaactccga
     1501 ggcgatcgaa accctgacgt cgtccacgcc ctcgagcttg cggactccgg cctcgatctt
     1561 ggcggcgcaa tcgccgcagt ccatcccggt gacccggtac tgggcgcgct cgcctgcagg
     1621 gcgctgtgtc atggcggaac catcctcatc acttcaccct agctcctcta gccacttgag
     1681 gatcaagccc gaaacgggcg gggaccgccg gacctcgcag gtcaccggcc gagccccatc
     1741 aacacgcccg ccgtcaccaa cccgaagatc gtcggcccag cggtcagggc catgccgaac
     1801 gcccggccgg aggcgccccg cgcgtagccc acggcgaagc acactcggcc cgccatgtag
     1861 aggaggacca ttgtgggcag gagcaccagc tcgccgtcgc gaagcacggc ggccaggatc
     1921 aagtgcgcgc ccatgaacag cacggtttgc tccagggagt tctgcaggat tgcgaccgga
     1981 accttgagct ccgggctggg gggcgcaaac gccgagccgc ggatgtcggc cggcgaagtg
     2041 aagcgacgtc cggagacccc gcggatcgcg atcgccagcc agagaaagac cggcagcgcg
     2101 gtcttcaggg cgtaaacaag acgctcgccg gcatcgaagc ccggagcgac gcccatcgac
     2161 tgggcgggca gccacgccca ggccgcggcc aaggtggcgc cggcgatggc cagtgcaagc
     2221 gccgatccgc gcacgattcc catttggtcg ggcgacaatg gcacgtccgg gcctccagaa
     2281 agccaagagt ctacgacctc tagctactag aggatcaagt tcgaatctta ggcgggttcc
     2341 gcctcttgct cctcaagtgg ctagagggta taaggtccct gcaaaccagt cttaatcgca
     2401 cccgagtcct tcatgccggc cgccctccgc ctcttcgcct tcgtcttgct gctgacggcc
     2461 acggcggcgc cgctccgcgc cctcgctcag gaggcgccgc ccgcgcaggt cgcttggcgg
     2521 ctgctcgact acatctccgt ggattatccc ggcgcggttc agggcgatcg ggtgatcagc
     2581 gcggcggaat acgccgagat gcaggaattt gccaagaccg tgcgggtgcg gctcgccagc
     2641 ctgccggcga ccccggcgaa ggccggcctc ctgcagggcg ccgacgccct ggccaccgag
     2701 atcgcgcata aggccccgcc cgccgaggtc gccgtcctcg cccgccgctt ggctggcgag
     2761 ttgctggccg cgtatcccac gccgcttgcc ccggcgacga cacctgacct ggcactcggc
     2821 gcgcggctct atgctgagca gtgcgcagcc tgccacggtg cgaccggcca tgcggacgga
     2881 ccgaacgcca aagggctcga ccccgcccca gtcgccttcg ccgaccccac gcgcgcccgg
     2941 gaacgcagcc tgttcggcct ctaccaggtg atcggccagg gcctggacgg caccgcgatg
     3001 gcgagcttcg cccacctcag cgacactgat cgatgggcgt tggcctttta cgccgggggc
     3061 ttcgcttttg acgctgacgc cgcacaggcc ggcgaggcgc tttggaaggc cgatccgaac
     3121 ctgcgcgccc gcttcactaa tctgcaggcg gtgacccagg ttacccccgc agccctcgct
     3181 gcggagctcg gcgagccccg cgccacccag ttgaccgcct tcctgcggcg ccagccgtcg
     3241 gctgtcacgc catcgggcga aggcagtctc gacgtggcgc gcaccaagct gcagcagagc
     3301 ctagcggcct atcaggccgg cgatccgaag cgggccgaaa atcttgccct tcccgcgtac
     3361 ctcgacgggt tcgagccggt ggagcccact ctcaaggcgc gcgacggcgc tctgatggtg
     3421 cgcattgaga ccgccatggg cgagttgcgc gcaagtgtgt cgtcgcgggc gccggcggcg
     3481 gacgtggcga ccaaggtcga acggctcatc gcgctcctga atgtcgccga acaggcgctg
     3541 gcccctgagc gcgccagcgc cgcctcaagc ttcgccggcg ccttcgccat cctgctgcgc
     3601 gagggcctgg aagccctgct gatcgtcgtc gccatgatcg ccttcctcgg caaggccgac
     3661 cggcgcgacg tgctccccta tgtccacgcc ggctggattt cggcgctggc cgccggcgcc
     3721 gccacctggg cggctgccac ttggctgatc tcgatcagtg gggcgagccg ggagctcatg
     3781 gaggggttcg gatcactggt atcggccgcc gtgctggtct cggtcggggt ctggatgcac
     3841 ggcaagtccc acgcggaagc ctggcagcgg tacatcaagg aaaagctctc ggcggcgctc
     3901 tctaaaggct cggcttggtt cctgttcggc ctggcgttcg tggttgtcta tcgcgaggtc
     3961 ttcgagacga tcctgttctt cgcggccctc tgggcccagg gcggccacct cgccttgatg
     4021 gccggggccg gcgtcgcaat ggtcttcctg gcggtgatcg cgcgcctgct gctcacctac
     4081 agtcgtcgcc tgccgatcac ccagttcttc gcttacagcg ccctgctgat cgccgtgctc
     4141 gcggttgtcc tggccggcaa aggcgtcgcg ggcttgcagg aggccggcct cctcgatgtc
     4201 cacccgctcg ccggtgcgcc gcggatcgag atgctgggcg tctacccgac ctgggagggc
     4261 ctctgggcgc aactggcgac cctcgcgctg ctcgtggtgg gcttccgcat cgccggccgc
     4321 aagaccgcca cggccgccta ggggacgttc gcttccagtt cgtcgagcca gatcttggcc
     4381 gtgccgtcgc tcggcgcgcg ccagtcgccg cgcggggaca gcgcgccgcc ggagatcacc
     4441 ttggggccat tcggcagcgc gctgcgcttg aactggctga tctcgaagaa gcggaagagg
     4501 aagacgccga gccacttctt gatggtcgcc aggtcgtagg cgttgcgcgt ctcctcgggg
     4561 aagttctccg gccacgcgcc ctcggccacg tcgcgccagg cgtgccaggc gaggaacgcc
     4621 accttcgagg ggcgcaggcc aaagcgggtg atgtggaaga ggttgaagtc ctgcaact
//
LOCUS       DAOMEO010000086         4669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2286_length_4669_cov_2.501734, whole genome shotgun sequence.
ACCESSION   DAOMEO010000086 DAOMEO010000000
VERSION     DAOMEO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_4669_cov_2.501734"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1406)
                     /gene="ccrA"
                     /locus_tag="PLH31_02990"
     CDS             complement(132..1406)
                     /gene="ccrA"
                     /locus_tag="PLH31_02990"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HQR88235.1"
                     /translation="MTTATLEKSPVKDLYEIGEIPPPFHVPKNMYAWSIRKERHGKPS
                     TAMQVEVVPTWEIGEDEVLVLVMAAGVNYNGVWAALGEPISPLDGHKQPYHIAGSDAS
                     GIVWAVGSKVKRWKLGDEVVIHCNQDDGDDEECNGGDPMFSTSQRIWGYETPDGSFAQ
                     FCRVQSRQLLQRPKHLTWEEAACYTLTLATAYRMLFGHKPHELRPGQNVLVWGASGGL
                     GVFAVQLAAVAGANAIGVVSSEDKREFVLSMGAKAVLNRNEFNCWGQLPKVNGPEFND
                     YMKESRKFGKALWQITGNKDVDMVFEHPGEQTFPVSVFVVKRGGMVAICAGTSGFNLT
                     MDARFLWMRQKRVQGSHFANLMQASAANQLVIDRRVDPCLSEVFPWNDIPLAHEKMLA
                     NQHLPGNMGVLVCAQRPGLRTFEEVQELSGPL"
     gene            1636..2364
                     /locus_tag="PLH31_02995"
     CDS             1636..2364
                     /locus_tag="PLH31_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQR88236.1"
                     /translation="MNPTLHVSRPAEAIILLELDAPPANGLGMALRRQLSDAVATVNA
                     DLSVRVVVLTGRGGVFCAGDDLREAAQRGPGQLDAVLGFNVLMDEVETCRVPVIAAID
                     GWCLGGGLELALSCDVRIASERALLAASGVNIGLMASVVRLPRLIGEARAKAHLLTGA
                     SFDAARALSDGVVTAVHPVDALLPAALDLAQRIATRAPLAVEAAKRAVAGAREDLAGL
                     VNSADHAEAVMAFMTKRSALFRRA"
     gene            2471..2713
                     /locus_tag="PLH31_03000"
     CDS             2471..2713
                     /locus_tag="PLH31_03000"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HQR88237.1"
                     /translation="MSNTPHELHEEFPEDAARIHALKTSDAHFAKLLEGYNEVNRAVH
                     RMETNIEAVADAVLEDFKKQRLELKDKIASALAKLA"
     gene            2742..3176
                     /locus_tag="PLH31_03005"
     CDS             2742..3176
                     /locus_tag="PLH31_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQR88238.1"
                     /translation="MIIQLAVATLMVLLTVTIHGVGLALLGKYLRLEARDEMSRHVPA
                     LSRRTLVFTLVLVVALFVLHGVEIWSYALLFMALGAIGDLETAVYFSTIAYAGIGFDD
                     AYISPAWRLVSGIEGVNGVLLMGWSTAFFVTVVTRLGGGFRP"
     gene            3262..>4669
                     /locus_tag="PLH31_03010"
     CDS             3262..>4669
                     /locus_tag="PLH31_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQR88239.1"
                     /translation="MSGKPYQHEPDAPWIFRTYAGHSTAEKSNALYRANLSKGQTGLS
                     VAFDLPTQTGYDPDHILSRGEVGKVGVPISHLGDMRQLFDQIPLDQSNTSMTINAPAA
                     WLLAMYVALADEQGADRKLLQGTTQNDLIKEYLSRGTYIFPPGPSLRLIGDMIAWCYL
                     EVPKWNPMNVCSYHLQEAGATPEQELAFALATAISVLDTVKAGGQVPEQDFEIVASRI
                     SFFVNAGVRFVTELCKMRSFTRLWDQILLERYGVQDKKARRFRYGVQVNSLGLTEQQP
                     ENNVYRILIEALAVTLSKDARCRALQLPAWNEALGLPRPFDQQWSLRLQQVLAYETDL
                     LEYEDLFDGSHVVEAKVAELSKGALEQLALIDGMGGAASAIDYMKTQLVASNAKRVRS
                     VETGDMTVVGVNRWTDTDVSLLSAGESAIETVDPRLEAGVVERIKAWRAARDAGAVEA
                     ALADLKAAAKDGRNIMEPS"
BASE COUNT          767 a         1519 c         1542 g          841 t
ORIGIN      
        1 gacctcggac ttatcctgga actgttcact ggcgctcgtc tctccggcgg ccatctctcc
       61 ggcgatcggg gaggcgcctc cggggagaga tacggacccg aaggtccgcc ggatggcctg
      121 aggcggctag atcagagcgg accgctcagc tcctgcacct cttcgaacgt ccgcagaccc
      181 ggccgctggg cgcagacgag cacgcccatg ttgccgggca gatgctggtt ggccagcatc
      241 ttttcgtgag ccagggggat gtcattccag gggaagacct ccgacaggca ggggtcgacg
      301 cggcgatcga tgaccagctg gttggcggcg ctggcctgca tcaggttggc gaagtgactg
      361 ccctggacgc gcttctggcg catccacagg aagcgggcgt ccatggtcag gttgaagccg
      421 ctggtgccgg cgcagatggc gaccatgccg ccgcgtttga ccacgaacac cgagaccggg
      481 aaggtctgct cgccagggtg ttcgaacacc atgtcgacgt ccttgttgcc ggtgatctgc
      541 cacaaggcct tgccgaactt ccggctctct ttcatgtagt cgttgaattc ggggccattg
      601 accttgggca gctggcccca gcagttgaac tcgttgcggt tcaacaccgc cttggcgccc
      661 atcgacagga cgaattcgcg cttgtcctcg cttgagacca cgccgatggc gttggcgccg
      721 gcgacggcgg ccagttgcac cgcaaagacg cccaggccgc ccgaagcgcc ccagaccagc
      781 acgttctgac cggggcgcag ctcatgcggc ttgtggccaa acagcatgcg ataggctgtg
      841 gccagggtca gggtgtagca ggcggcttct tcccatgtca ggtgcttggg gcgctgcagc
      901 agctgacgcg actgcacccg gcagaactgg gcgaagctgc cgtccggcgt ctcgtagccc
      961 cagatgcgct ggctggtcga gaacatcggg tcgccgccgt tgcactcttc gtcgtcgccg
     1021 tcgtcctggt tacagtggat gacgacctcg tcgcccagct tccagcgttt gaccttggag
     1081 cccaccgccc agacgatgcc cgaggcgtcg ctgccagcga tatgataggg ctgcttgtgg
     1141 ccgtcgagcg ggctgatcgg ctcgccgagc gcggcccaga cgccgttgta gttgacgccg
     1201 gcggccatca cgaggaccag cacctcgtcc tcgccgatct cccaggtggg cacgacctcg
     1261 acctgcatgg cggtggacgg cttgccgtgg cgctccttgc ggatgctcca ggcgtacatg
     1321 tttttcggca cgtggaaggg tggcgggatt tcgccgatct cgtagagatc cttcacgggc
     1381 gacttttcta gcgtggcggt cgtcatgtca ctccccattc tttcggcgct gcaacaacgt
     1441 ccgaaagctc aactcggctg cgcgcggggc ggtcgaaatg gtgaaaatac ctcgccgacc
     1501 cgtctttttt ggtcccgagg cggataaatc gaccccgaag tcgcttaagc tgagacaagt
     1561 gtttgaaact ggcaagcgga atcttgtgcg acgcaacata ctggtcaggc gggctgcgat
     1621 cggcgaggct tgggcatgaa cccgaccctc catgtctccc gacccgccga agccatcatc
     1681 ctgctggaac tcgacgctcc gcccgccaac gggctgggca tggcgctgcg tcgtcagctt
     1741 tcggacgccg tcgcgacggt caacgcagat ctttcggtgc gggtcgtggt gctgaccggc
     1801 cggggcggcg tcttctgtgc gggcgatgac cttcgcgagg cggcgcaacg cggcccgggc
     1861 cagctggacg cggttctggg cttcaacgtc ctgatggacg aggtcgagac ctgccgggtc
     1921 cccgtcatcg ccgcgatcga cggctggtgt ctgggcggcg ggctggagct tgcgctgtcc
     1981 tgcgacgtcc gcatcgcaag cgagcgcgct ctcttagcgg cctcgggcgt caatatcggc
     2041 ttgatggcgt cggtggtcag gcttccccgc ctgatcggcg aggcgcgggc caaggcgcat
     2101 ctgctgacgg gagcgagctt cgatgcggcg cgggcgctgt cggacggcgt ggtgacggcg
     2161 gtccatcccg tcgacgcgct tttgccggcg gctctggatc tggcgcagcg catcgccacg
     2221 cgcgcgcccc tggccgtcga ggccgccaag cgggcggttg cgggcgcccg ggaggatctg
     2281 gcggggctgg tgaacagcgc tgatcacgcc gaggctgtca tggccttcat gaccaagcga
     2341 tcagccctgt ttcgcagggc ctgatcccaa agcccccagc gaaattgatc gaccgcaagg
     2401 ctgccgtccg ccgggcgcgc aggatggcgc cggcgccaag ctgggcgtca tttcctggaa
     2461 gtcccgaccg atgagcaaca ccccccacga actgcacgaa gagtttcccg aggacgccgc
     2521 gcggatccat gcgctaaaga ccagcgacgc ccacttcgcc aagcttcttg agggctacaa
     2581 cgaggtcaat cgcgccgtgc accgcatgga aaccaacatc gaggcggtgg ccgacgcggt
     2641 tcttgaggac ttcaagaagc agcgtcttga gctgaaggac aagatcgcct ccgccctggc
     2701 caagctggcc tgatccccgc caggccgacg ctcacgtgca catgatcatc caactggcgg
     2761 tcgccaccct gatggtgctg ctgaccgtca cgatccacgg cgtcggcctg gctctactgg
     2821 gcaaatacct gcgcctggag gcgcgggacg agatgtcacg gcatgttccc gccctgtccc
     2881 gacgaaccct ggtcttcacc ctggtgctgg tggtggcgct gtttgtgctg cacggggtcg
     2941 agatctggtc ctacgccctg ctgttcatgg ccctgggcgc gattggcgat cttgagaccg
     3001 ccgtctattt ctcgaccatc gcctatgccg ggatcggctt cgatgacgcc tatatctcgc
     3061 ccgcctggcg gctcgtcagc ggtatagagg gcgtcaatgg cgtgctgctg atgggttggt
     3121 ccaccgcctt cttcgtcacg gtcgtgaccc ggttgggcgg gggctttcgt ccgtaaggca
     3181 ggcttcgctt ttgcgatatt gcatcgcagc aaaaccgggt tcatgatcgg cggtaatcaa
     3241 gaacacaggt gggagcgcag gatgtcaggc aagccgtatc agcacgagcc ggatgcgccg
     3301 tggattttcc gcacctatgc cggccattcg acggccgaga agtccaacgc cctctatcgg
     3361 gccaacctgt ccaagggcca gaccgggctg tcggtggcgt tcgacctgcc gacccagacg
     3421 ggctacgatc ctgaccacat cctgtcgcgc ggcgaagtgg gcaaggtcgg cgtgccgatc
     3481 agccatctgg gcgacatgcg ccagctgttc gaccagatcc cgctggacca gtccaacact
     3541 tcgatgacca tcaacgcccc agccgcctgg ctgctggcga tgtatgtggc cctggccgac
     3601 gagcaggggg ccgatcgcaa gctgctgcag ggcaccaccc agaacgacct gatcaaggag
     3661 tacctctccc gcggcaccta catcttcccg ccgggcccgt ccttgcggct gatcggcgac
     3721 atgatcgcct ggtgctatct ggaggttccc aagtggaacc cgatgaacgt ctgcagctat
     3781 cacctgcagg aagccggcgc gacgccggag caggagctgg ccttcgccct ggccaccgcc
     3841 atttcggtgc tcgacaccgt caaggcgggt ggccaggtgc ccgagcagga cttcgagatc
     3901 gtcgcctcgc gcatcagctt cttcgtcaat gccggcgtgc ggttcgtcac cgagctgtgc
     3961 aagatgcgta gcttcacgcg cctgtgggac cagatcctgc tggagcgcta tggcgttcag
     4021 gacaagaagg cgcgccgctt ccgctacggg gttcaggtca attcgctggg gctcaccgag
     4081 cagcagcccg agaacaacgt ctatcgcatc ctgatcgagg ccctggcggt gacgctgtcc
     4141 aaggacgccc gctgccgcgc cctgcagctg cccgcctgga acgaggcgct tggcctgccg
     4201 cgtccgttcg accagcagtg gtcgctgcgc ctgcaacagg tgctggccta cgagaccgac
     4261 ctgctggaat acgaagacct gttcgacggc tcccacgtgg tcgaggccaa ggttgccgaa
     4321 ctcagcaagg gcgcgctgga gcaactggcc ctgatcgacg ggatgggcgg cgccgcgagc
     4381 gccatcgatt acatgaagac ccagctggtg gcgtccaacg ccaagcgcgt gcgctcggtc
     4441 gagaccggcg acatgaccgt ggtcggcgtc aaccgctgga ccgataccga tgtctccctc
     4501 ctgtcggcgg gcgaaagcgc gatcgagacc gtcgatccgc gtcttgaggc cggcgtcgtc
     4561 gaacgcatca aggcctggcg cgcggcccgc gacgcgggcg ccgtcgaggc ggctctcgcc
     4621 gacctgaagg cggcggccaa ggacggtcgc aacatcatgg aaccctcga
//
LOCUS       DAOMEO010000087         4651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2289_length_4651_cov_2.366841, whole genome shotgun sequence.
ACCESSION   DAOMEO010000087 DAOMEO010000000
VERSION     DAOMEO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_4651_cov_2.366841"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..2482
                     /locus_tag="PLH31_03015"
                     /pseudo
     CDS             1..2482
                     /locus_tag="PLH31_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519059.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2616..>4651
                     /gene="pbpC"
                     /locus_tag="PLH31_03020"
     CDS             2616..>4651
                     /gene="pbpC"
                     /locus_tag="PLH31_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HQR88240.1"
                     /translation="MKGERRAKYVPLLSKALVGFLTVQVVLAALSAIFPPDMTRARQS
                     SPVALDRRGTWLRALPVEDGRWRIRADLERTDQTFRKRLIAVEDGRFYQHLGVDPLAV
                     VRAFGSAVLNGRPTSGASTLTMQTARLLEPRPRNLGAKLIEMIRAVQLESRLTKRQTL
                     ALYLTLAPYGGNLEGVRAASLAYFGQEPSSLTDGQQALLIALPQSPEARRPDRRPEAA
                     RLARRAVLDKMVRAGVLTEAAAVEADAEPLPRRAPFPALAWNVAGELARAAPASQASV
                     ITTLDADLQARLEPMAAAIAASQGADATAAILVVEIKGRAVRGAVGSAGRDRAGGWVD
                     MTRALRSPGSALKPFIYAMAMDDGLVAADTQLADSATRFADYQPENFDRVFHDKVTVR
                     EALTHSLNVPAVATLERLGPEAFAGRIEAAGAHLARPKSELKAAGLALALGGEGITLR
                     DLALLYAALGDGGLAKPLAWTEADARLRETSGGTRLVRPEAARQVLDILREAPAPRGR
                     APSALTRGGPAMAFKTGTSYGFRDAVAAGVVGGYAIIVWTGRADGGARGGLTGRDAAL
                     PLLFDVADVVGAPAAAPRPIAPKSAPQALQTLRAQTEGPRLIFPPDGAAVQVEALGPR
                     SRGLVLAAGGEDLTWYVGGAPLAADPVSGRVVWRPAAPGFYRLSVVDGQGRKASA"
BASE COUNT          660 a         1621 c         1689 g          681 t
ORIGIN      
        1 acgaccgtgc gcctgaagac ctcggccgcc tggggcggcg gggcctatgt gatggtcagc
       61 gtcatccagc cgcgcgaccc ggtcgcctcg cccaagccgc gccgggccct gggcctggtc
      121 tatgtgccgc tcgatcccaa ggaccggaaa ctgaccgtcg atctgggcac gcccgtgaag
      181 atcgactcca aggcgccggt cgaaatcccc gtggcggtga agggcctggg cctgggccag
      241 cgcgcccacg tcaccatcgc ggcggtcgac gagggcattc tgcgcctgac ccgccaggac
      301 agtccagatc cggtgaaatg gtatttcggc aagcgcgccc tgggcgtcga ctatcgcgac
      361 gactacggcc gcctgctcga tcccaatctg ggcgcgcccg ccaatgtcaa tttcggcgcc
      421 gacgaggtcg ggggcgaggg tctgaccgtc accccgatca agaccgtggc cctgtggtcg
      481 ggcgtggtcg agaccggcct tgatggcaag gcggtgatca ggctgcccgc cgccgacttc
      541 aacggtgagc tgcgtctgat ggccgtggcc tggaccgata ccgccgtcgg ctcgggctcc
      601 aagcccctga ccgtgcgcca gccggtggtg gccgacctga acctgccgcg cttcctggcc
      661 cccggcgaca aggccatggc gacgctggaa ctgcacaatg tggaaggcaa acccggcgcc
      721 tataccgccg aggccttctc gtcgggcggg ctgaaggtgg ctttcaagaa ggtgttccag
      781 ctaatcctgg gacaacggat cgccgagaag atccccttcc tggcccccga cgtcaccggc
      841 atcggcaaga tcggcttcaa ggtggccggg ccgggcttta cgaccagcaa ggactatccg
      901 atccagacgc gcctcggttg gggcgacgtg gtacgcacca ccacgcagct gcagcagccg
      961 ggcgcggcct atacgccgac ggcggatctg atgagcggtc tggcggccgg ggatgtgacg
     1021 ctgcaggtca gctattcgcc gttcaagggc ttcgacccgg cggcgatcgc ggtggccctg
     1081 caacgctatc cctatggctg caccgagcag ctggtctcga cggcttatcc gctgctctac
     1141 gcccagagcc tgaccagcga cccgaagctg aaacgcaccc cgacggccct ggccaatgcg
     1201 gtgggcaagc tgctggatcg ccagaccctc gacggggcct ttggcctgtg gcgggtgggc
     1261 gatggcgagg ccgacgcctg gctgggcgcc tatacgaccg atttcctgct ggaagcgagg
     1321 gcccagggcg tggccgtgcc ggacgcggca atcgacaagg ccatgaacgc catgcgccag
     1381 atcagccgtc ccgacggctg ggcctcggtc tcgtaccgga tggaatatcc cgagtggtgg
     1441 gcgtcttcgc cggaggcctc caagaaggcc accgaacgga tgcgtcgccg ggcctcggcc
     1501 tatgcgctgt atgtgatggc caagggcggc aagggcgacc tggcccgcct gcgctggtgg
     1561 cacgacgtgc agatgaagga cgaagaccag cccatggcga aggcctatgt cgccgccggt
     1621 ctggcccgga tgggcgacaa tgcccgggcc cgttcggcca tgcgccaggc ggtccggtcg
     1681 ctgggctatc gtgacgagca ggactggtac cagagcccgc tgcgcgacgt ggcgggactg
     1741 acggccctgg ccgtcgaggc gggccagggt gatgtcgccc gccagctgca gggccggctg
     1801 gagaatgtgg tcaaggatcc cgacgcgctg aacacccagg aacaggctgc cgtgcttcac
     1861 gcggcctacg ccctgctgaa ggctgcgggc ccgatctcga tcaacgccac gggggccctg
     1921 gccctgccgc cagccggcgg cgcgccgcgc tgggcggtgg gcaagctggc cgatagtcgc
     1981 ttcgtcaaca ccggcaaggg cgccctgtgg cgtaccgtca gcgttcgcgg cacgcccacg
     2041 gtcgcgccgg gggcggaagc caatggcctt tcggttaaca agcgcttgct gtcgatgacc
     2101 ggggcggcgg tcgatccggc caccatccgc cagggcggtt tcgggccgct cgatgcaggc
     2161 ccgctcgacc gccctggtgg tcgacgacgc cctgccggcc ggcttcgaga tcgagacgac
     2221 gctcggcgcc gacgacgccc agaacgggcc ctacagattc ctgggcgaac tgacgggggc
     2281 cgacgtgcag gaaagccgcg acgaccgctt catcgcggcc atggacctgg aaggaaacaa
     2341 gcccttcgcc ctggcctatg tggcgcgggc ggtgaccccg ggcgagttct tcctgcccgg
     2401 cgccgtggcc aaggacatgt accggcccgc gatcaccgca cggtcggacg ccggccgggc
     2461 ggtgatcgcg ccggggcagt agtgatgtcg aatgtgggct cccttccccc tttgatgggg
     2521 gaagggttgg ggatgggggt gaaagcctgt ccggttgagg cgcggccttc gtgaaatcgt
     2581 ccgacgctgt cacccccact cccgacctct cccccatcaa gggggagagg agagcgaaat
     2641 acgttccgct tctgtcaaag gcgctcgtcg gcttcctcac cgtccaggtc gtcctcgctg
     2701 ccctgagcgc catcttcccg ccggacatga cccgcgcccg gcagtcctcg cccgtggcgc
     2761 tggaccggcg cgggacgtgg ctgcgggccc tgccggtcga ggacggccgc tggcggatcc
     2821 gggctgatct ggagcgcacc gaccagacct tccgtaagcg tctgatcgcg gtggaggacg
     2881 gccggttcta ccagcacctc ggcgtcgatc ccctggcggt ggtccgggcg ttcggttcgg
     2941 cggtgctgaa cggtcgcccg acctccggcg cctcgaccct gaccatgcag accgcgcgcc
     3001 tgctggagcc gcgcccgcgc aatctgggcg ccaagctgat cgagatgatc cgcgccgtgc
     3061 agctggaatc gcggctgacc aagcgccaga ccctggccct gtacctgacg ctggcgccct
     3121 atggcggcaa cctggaaggc gtgcgcgccg ccagtttggc ctatttcggc caggagccga
     3181 gcagcctgac cgacggccag caggctttgc tgatcgccct gccgcaatcg cccgaagccc
     3241 gccgtccaga tcgccggccc gaggcggcgc ggctggcgcg gcgggcggtt ctggacaaga
     3301 tggtccgcgc cggggttctg accgaggccg ccgctgtcga agccgacgcc gaacccctgc
     3361 cgcgccgtgc gccgttcccg gccctggcct ggaatgtggc cggcgaactg gcccgcgccg
     3421 cgccagcctc tcaggccagt gtgatcacca ccctcgacgc cgacctgcag gcgcggctgg
     3481 agccgatggc cgccgccatc gcggcctcgc agggggccga cgccaccgcc gcgatcctgg
     3541 tggtggaaat caagggccgg gcggtgcgcg gcgcggtggg atcggcaggc cgcgaccggg
     3601 ccgggggctg ggtggacatg acccgcgccc tgcgctcgcc gggttcagcc ctgaaaccct
     3661 tcatctacgc catggccatg gacgatggcc tggtcgccgc cgacacccag ctggccgaca
     3721 gcgccacgcg ctttgccgac tatcagcccg agaactttga ccgcgtgttc cacgacaagg
     3781 tcacggtccg cgaggccctg acccattcgc tgaacgtgcc ggccgtggcg acgctggagc
     3841 ggctgggtcc cgaggccttc gccggccgga tcgaggcggc cggcgcccat ctggcccggc
     3901 cgaaatcgga gctgaaggcg gcgggcctgg ccctggccct gggcggggag gggatcaccc
     3961 tgcgcgacct ggccctgctg tatgccgccc tgggcgacgg cggcctcgcc aagccgctgg
     4021 cctggacgga agccgacgcc cggctgcggg agacgtccgg cggaacccgg ctggtgcgcc
     4081 cggaggcggc gcgacaggtg ctggatatcc tgcgcgaggc ccccgcgccg cgtggccggg
     4141 cgccctcggc cctaacccgg ggcggacccg ccatggcctt caagaccggt acctcctacg
     4201 gcttccgcga cgccgtggcg gctggggtgg tcggcggcta tgcgatcatc gtctggaccg
     4261 gccgcgccga cggcggcgcg cggggtgggc tgaccgggcg agacgcggcc ctgccgctgc
     4321 tgttcgacgt ggccgacgtg gtgggcgccc cggccgccgc gccgcgtccg atcgcgccga
     4381 agtcggcgcc tcaggctttg cagaccctgc gcgcccagac cgaaggtccg cgcctgatct
     4441 tcccgccgga cggcgcggcc gtgcaggtcg aggccctggg gccaaggtcg cgcgggctgg
     4501 tgctggcggc gggcggtgag gacctgacct ggtatgtcgg tggcgcacct ctggcggccg
     4561 atccggtcag cggtcgggtg gtctggaggc cggcggcgcc ggggttctac cggttgtcgg
     4621 tggtggacgg gcaggggcgc aaggcgtcgg c
//
LOCUS       DAOMEO010000088         4648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2291_length_4648_cov_2.677988, whole genome shotgun sequence.
ACCESSION   DAOMEO010000088 DAOMEO010000000
VERSION     DAOMEO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_4648_cov_2.677988"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="kdpC"
                     /locus_tag="PLH31_03025"
     CDS             <1..610
                     /gene="kdpC"
                     /locus_tag="PLH31_03025"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HQR88241.1"
                     /translation="SRRRPRHRLGRDRRQSYRPALVSMGLFTALLGLAYPLAITGVAQ
                     AAFPDQANGSLVRDAKGTVIGSALIGQPFAAAKYLHPRPSAAGAGYDASASSGSNMGP
                     MNEALAERVKTDADALRAENPGVVIPADAVTTSGSGLDPEISPAFARLQAPRIAKARG
                     LTTPQVEAVIDANVQGRLLGFIGQPRVNVLAVNRVLDARAPG"
     gene            741..2733
                     /locus_tag="PLH31_03030"
                     /pseudo
     CDS             741..2733
                     /locus_tag="PLH31_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615360.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
     assembly_gap    2734..2743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2744..3097
                     /locus_tag="PLH31_03035"
     CDS             <2744..3097
                     /locus_tag="PLH31_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88242.1"
                     /translation="LLEQAIVNILENAIAYSPAEARIEMAAYEDRANVVISIEDEGRG
                     IATAELERVFEKFRRMEEPSDRNQGAGLGLSIAKGFIDAMGGRIAAASPIHDGKGTRI
                     LISLPKAVATPHHLL"
     gene            3105..>3533
                     /locus_tag="PLH31_03040"
     CDS             3105..>3533
                     /locus_tag="PLH31_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88243.1"
                     /translation="MSISRPRILVIDDEPQIHRFLGPALDAAGYEPLRADSGQEGLRG
                     VATWSPDAVVLDLGLPDMDGKDVLIRARDFYDGPILILSARDREAEKIQALDHGANDY
                     LEKPFGVGELLARLRAALRQRASTRLPPGPIRAGDVEIDLD"
     assembly_gap    3534..3543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3544..3801
                     /locus_tag="PLH31_03045"
     CDS             <3544..3801
                     /locus_tag="PLH31_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQR88244.1"
                     /translation="RVTRGGAVVKVSPKEFELLARLASGRGKLLTHKDLLVAVWGPAH
                     ATDTQYLRVFVGQLRQKLEIDPAHPKIIETEPGVGYRFIAD"
     gene            complement(4460..4648)
                     /locus_tag="PLH31_03050"
                     /pseudo
     CDS             complement(4460..4648)
                     /locus_tag="PLH31_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          683 a         1564 c         1670 g          711 t
ORIGIN      
        1 gtctcgccga cgccctcgac atcgactcgg ccgggacagg cgccagtcat atcgtcccgc
       61 cctggtttcc atgggccttt tcaccgccct gctgggcctg gcctacccgc tggcgatcac
      121 cggcgtcgcc caggcggcgt tcccggatca ggccaatggc agccttgtcc gtgacgccaa
      181 ggggaccgtc atcggctcgg ccctgatcgg ccagccgttc gcggcggcga aatatctgca
      241 cccccgcccg tcggcggccg gcgccggcta tgatgcctcg gcctccagcg gctccaatat
      301 gggcccgatg aacgaggccc ttgccgagcg ggtcaagacc gacgccgacg ccctgcgcgc
      361 cgagaacccc ggcgtggtga tccccgccga cgccgtcacc acctcgggct cgggcctgga
      421 tcccgagatc tcgccggcct tcgcccgcct gcaggcgccc cgcatcgcca aggcgcgcgg
      481 cctgacgacg ccgcaggtcg aggcggtgat cgacgccaat gtccaaggtc gtctgctggg
      541 cttcatcggc cagccgcgcg tcaatgtgct ggcggtcaac cgggtgctcg acgcccgcgc
      601 gccgggctag acgcttgccg gccgacgaac ccaaaaggcc cgatccagag gcgcttctgg
      661 ccgccgccaa caaggcgggc cgggggcgcc tcaaggtgtt tctgggcatg gcgccgggcg
      721 tgggcaagac ctacgagatg ctgcgcgccg cgcggcgtcg caaggccgag ggcaaggacg
      781 tggtcgtcgg tctggtcgag acccacggcc gccgcgagac cgaaagcctg ctgcgcgaca
      841 tggaggtcat gccgcgtcgg ccgatcgatc atcgcggcca cgtgatgatg gagttcgacc
      901 tcgacgcggc cttggcgcgc aagccggccc tgctgctggt cgacgaatac gctcacgcca
      961 acgcgccggg ctcgcgccat cccaagcggt ggcaggatgt ggaagagctg ctggcggccg
     1021 gcatcgatgt ctggacgacc ctgaacgtcc aacatctgga aagccttgtc gatgtggtgc
     1081 agcgcatcac cggcgtgcgc cagcgcgaga cggtgcccga cagcgctctg tcgcgggccg
     1141 acgccatcga ggtgatcgat atcacccccg acgagctgcg cgagcggatg gcggcgggca
     1201 aggtctatgt gcccgagacg gcgcggctgg cctcggagaa cttcttcaag gtcgagaacc
     1261 tgaccgccct gcgggaactg gccctgcgcc gcgccgccca gacggtggac gaccagctgg
     1321 tcgccgccat gcgcgaaaag ggggtcgagg gcccctgggc ggcgggcgag cggatcctgg
     1381 tgctgatcgg cggcgacgcc atgtcggcgg ggctggtccg taccggccga cgcctgtcgg
     1441 acatgatgat ggatgcgccg tggaccgtca cccatgtgga caggcccgac cacgccccgc
     1501 cgggtccggt ggccggcgcc cgggttcgcg acgcgctgaa gctggccgag cagctgggcg
     1561 gatcgaccgt ggtgctgacg gggtcggata tcgtcgccac ggtgatggcc tatgcccggc
     1621 gcaacaacgt cacccagatc gtcatcggca agtcggccga cagccgctgg cgcgagatgc
     1681 tgggtcagtc cctggcggcg gccctactga gagaggcgcg cggcgcggcg atccatgtgg
     1741 tcaccgaggc ggcgcatgag gctgcgcccg agccgcccaa gccccgcgct cggccgccct
     1801 ggcgcgccta tttcacgggc gcggctttcg tcggcgtcgc caccttgtgc gcctggggtc
     1861 tggacgtggc gttcgacagc gtggacctgg ggatgatctt cctggcggcg gtgctggcgg
     1921 ccggtgttcg gcacggcctg cgccccgcct tggcggcggc gaccgtcgcc tttctcgtct
     1981 acaattatct gttcctggag ccgcgctaca gctttcagat cggttcgccg accgacgtcc
     2041 tgaccctgat cgtcttctgg gctgtggcgc tggcggccgg tgggcttgca gggcgggtgc
     2101 gtgatcaggg acaggacgcc cagcggcgcg cctcggcggt gtcggccctg ctggcggcca
     2161 gccgcatctt ctcggccgcc gccgatcgcg gcgccaccgc ccgggccatc gccgaccagg
     2221 cggcggccgc agcgggcgcc aaggccatgg tgctgttgcc gcgcgatggc gagctggagc
     2281 ccgccgccgg cgcgccgacc ctggaaccgc tcgacgccgc cccgatggcc gccgcgcgct
     2341 gggcctggga gaagggcgag ccggccggct ttggcaccgg caccctgccg cagacccgct
     2401 ggaccttctg gccgctggac ggcgtgcggt cccgcgccgg cgtggccggt gtcgaggccg
     2461 gggtgatggt ggccggctcc gacaccgagc gtctggttct ggccttgctt gagcagggcg
     2521 ccgtggctct ggaacgggcc gaactggcga ccgaggccgt cgagaccgag accctgcgcc
     2581 gggccgaccg ctttcgctcg gcgctgctga actcgatcag ccatgacctg cgcacgccgc
     2641 tctcgaccgt gctgggcgcc tccagcaccc tgatcgacta tggcaagacc ctgacgcccg
     2701 ccgtgcgcgc tgacctgacc ttgagcatcc gcgnnnnnnn nnnctgctgg agcaggccat
     2761 cgtcaacatc ctggaaaacg ccatcgccta tagcccggcc gaggcgcgca tcgagatggc
     2821 ggcctatgag gatcgcgcca atgtggtgat cagcattgag gacgaggggc gcggtatcgc
     2881 gaccgccgag ctggagcggg tgttcgaaaa gttccgccgc atggaggagc cgagcgaccg
     2941 caatcagggc gcaggcctgg gcctttcgat cgccaagggc ttcatcgacg ccatgggcgg
     3001 ccgcatcgcc gccgccagcc cgatccatga cggcaagggc acgcgcatcc tgatcagcct
     3061 gcccaaggcg gtcgccacgc cgcatcacct gttatagcgg cgccatgagc atctcgcgtc
     3121 cacgcatcct ggtcatcgac gatgagcccc agatccaccg cttcctgggt ccggcgctcg
     3181 atgcggccgg ctatgagccc ctgcgcgctg acagcgggca ggagggcctg cggggcgtgg
     3241 cgacatggtc gcccgacgcg gtggtgctgg acctgggcct acccgacatg gatggcaagg
     3301 atgtgctgat ccgggcgcgc gacttctatg acggtccgat cctgatcctg tcggcccgcg
     3361 atcgcgaggc cgagaagatc caggctctgg accacggcgc taacgactac ctggaaaagc
     3421 ctttcggggt gggcgagctg ctggcccgtc ttcgcgccgc tttgcgccag cgcgcctcca
     3481 ccaggctgcc gcccggacca atccgggccg gcgatgtcga gatcgatctg gacnnnnnnn
     3541 nnncgggtta cccgaggcgg cgcggtggtg aaggtttcgc ccaaggaatt cgagctgctc
     3601 gcccgcctgg cttcggggcg cggcaaattg ctgacgcaca aggacctgct ggtcgccgta
     3661 tggggccccg cccacgcaac ggacacccag tatctgcgcg tcttcgtcgg ccagctgcgc
     3721 caaaagctgg aaatcgatcc ggctcatccg aagatcatcg aaaccgagcc tggggtcggt
     3781 taccgcttca ttgcggacta gccaatgtgc gacgagcatg gaccatgccg ttcgagccga
     3841 tctttggcat gtgaagcatg gccgagtgaa tgtccggaag ttggcgcgct gtgaagttcc
     3901 gttcctgttg agggtttcgg atgccggccc gtgcactggg tctgcggccg acgctgaggg
     3961 ccggctttcg aaagccttca ggggaggaag ccgcggtcag tacgccgagc gtgtcaggct
     4021 atggcgatag cggttgtcgc tgcggtgctg acgatagccc aataggcctg ggcagtggcc
     4081 acgaagagcc ccaaacgttc aacatgtcgg tccctgagcg gtgttagggg ccgacattgg
     4141 cggtcgcgga acggcgcttg aggcgcggct ggacgcgagg cgcagcaggc tctgcgccaa
     4201 ggccgggggc gatggcgggc atgggcgcgg acgatcgttg tggctagact catgagctgt
     4261 cacaccacag tggcgcgaag ggggcgggcg cgccaggcgc cgctgcggcc gatgacctcg
     4321 atgatccgca cggcgccgcc gcacgggcag gtcagggccg ctgtggcctt gggctcactg
     4381 ggtccttgtg cacggcggcc ggatgcggcg ttcgctccag gatcagggcg cgcagggtct
     4441 tcaagggcac ggcgttggga cccttggcta gcaggccata gtggcggatg cggtggaagc
     4501 cggacggcag cacgtggatc aggaaccgcc ggatgaactc ggccggcgac agggccatgg
     4561 tggccggccc cccgaacggt ggcttggcgt agaccaccca ttcggcgcga cgcaacgggg
     4621 cgagataggc ggcgagggcc tcgggcgc
//
LOCUS       DAOMEO010000089         4645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2295_length_4645_cov_2.737255, whole genome shotgun sequence.
ACCESSION   DAOMEO010000089 DAOMEO010000000
VERSION     DAOMEO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_4645_cov_2.737255"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLH31_03055"
     CDS             complement(<1..159)
                     /locus_tag="PLH31_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR88245.1"
                     /translation="MLRTLTLTLLAATSLTACTLAPPHERPTLPVAQSWPTAEPAGTV
                     SAADLGWRG"
     gene            complement(161..>1779)
                     /locus_tag="PLH31_03060"
     CDS             complement(161..>1779)
                     /locus_tag="PLH31_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88246.1"
                     /translation="KGFFGWFNRSFNDLSGRYQGSVRGILGKSGRWMVVYGAIIVVMA
                     FLFIRMPTSFLPEEDQGTIYTLVQLPAGATDERTQGVLKQVENHFLADKAVKSVFTVS
                     GFSFAGAGQNAGLAFISLKPFEERKSADLKAPAVAGRAFGAFSQIRDAMVFPIVPPAV
                     QELGNASGFDFQLQDTGGVGHEALINARNMMLGMAAQNPVLAGVRPNGQDDTPQLRID
                     VDQARAGAMGLTTADINAALSTALGGSYVNDFIDRGRIKKVYVQADAPFRMKPEDLDR
                     WYVRNSSGAMVPFSAFATTAWTYGSPRLERYNGLSSVNIQGTPAPGQSSGAAMAAMEE
                     IAAKLPPGIGFEWTGLSAQERESGNQAPALYAISILVVFLLLAALYESWSIPLSVIMV
                     IPLGVVGALLATTLRGLSNDVYFQVGLLTTMGLAAKNAILIVEFAKDLHEKGKPLIEA
                     TLEAVRIRLRPIIMTSLAFIFGVLPLAISNGAGSGSQHAIGTGVIGGMISGTLLAIFF
                     VPLFFVVVETVFKPKHKRVDETPAIPAEGH"
     assembly_gap    1780..1789
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1790..3292)
                     /locus_tag="PLH31_03065"
     CDS             complement(<1790..3292)
                     /locus_tag="PLH31_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88247.1"
                     /translation="MLSRFFIDRPIFAWVIAIVIMLAGLLAIRTLPIAQYPSIALPQV
                     SVSANYPGASAKTVEDSVTQVIEQKMKGLDGLEYMSSTSDSSGAATTTLTFKAGTDVD
                     IAQVQVQNKLQTATALLPQEVQQQGLSVVKSTRNFLMVVGLYSKAPGTTNVDLADYIA
                     ANIQDPLSRVEGVGDVQLFGAQYAMRIWLDPNKLASFSLTPSDVTAAIRAQNAQVSAG
                     QLGGTPAAPGVALNATISAQSRLQTPEQFGAIIIRNTTGGAIVHLRDVARVELGAESY
                     VFNAHFNGAPAAGLAVKLAPGGNALDTAEAVKARMTELSKSFPAGYEYVVPYDSTPFV
                     EQSIHEVVKTLVEAIILVFIVMFLFLQNWRATLIPTIAVPVVLLGTFGILAVFGYSIN
                     TLTMFGVVLAIGLLVDDAIVVVENVERVMSEEGLSPVEATRKSMTEISGALIGIALVL
                     SAVFVPMAFFGGSQGVIYRQFSITIVSAMLLSVVVALVLTPALTATLLKPV"
     gene            complement(3305..4450)
                     /locus_tag="PLH31_03070"
     CDS             complement(3305..4450)
                     /locus_tag="PLH31_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR88248.1"
                     /translation="MQFQRSTASAVVLAAISLTLAACGQKGAEGAPGMGGPSDVGVVI
                     ARTEPVSLTTELSGRTSAYLVSEVRPQVSGIVKARLFQEGGYVRAGQALYQIDPATYQ
                     AAYNSAAAGLAQAQAAYTAAKLKADRFGALVATGAVSKQDNDDAQAASQQAAANVAAQ
                     KAAVDAARINLGYTRVLSPISGRIGKSSVTPGALVTASQAAALATVQDTSRVYVDLTQ
                     SSAELLKLQRDLTNGSVGRSGSAQVTLKLEDGSTYPTPGKLEFSDVTVDPTTGSVGLR
                     ATFANPNGVLLPGMYVRAVLGTVTAQNGMLIPQPAVSRDPKGNATVMVVGAKNLAEPR
                     PITVRQMVGDKWLVTSGLKPGDQVIVEGLQKVRPGAPVKPAVITAQR"
BASE COUNT          751 a         1429 c         1694 g          761 t
ORIGIN      
        1 cccgcgccag ccgagatcgg cggccgagac ggttcccgcc ggctcggccg tgggccagct
       61 ttgggcgacc ggcagggtcg ggcgctcgtg cggcggggcc agggtgcagg cggtcagcga
      121 ggtggccgcc agcagggtca gtgtcagtgt gcgaagcatg tcagtggccc tcggccggga
      181 tcgcgggcgt ctcgtcgacg cgcttgtgct tgggtttgaa gaccgtctcg acgaccacga
      241 agaacagcgg gacgaagaag atcgccagca gggttcccga gatcatgccg ccgatcacgc
      301 cggtgccgat ggcgtgctgg ctgcccgagc cggccccgtt ggagatcgcc agcggcagga
      361 cgccgaagat gaaggccagc gaggtcatga tgatcggccg cagacggatc cgcaccgctt
      421 ccagggtggc ctcgatcagg ggcttgccct tttcgtgcag gtccttggcg aactcgacga
      481 tcaggatggc gttcttggcc gccagaccca tggtcgtcag cagtccgacc tggaagtaga
      541 cgtcgttgga cagaccgcgc agggtggtgg ccagcagggc gccgacgacg cccagcggga
      601 tgaccatgat caccgacagc gggatcgacc agctctcgta cagcgccgcc agcagcagga
      661 acaccaccag gatcgagatg gcgtacaggg cgggggcctg gttgccggac tcgcgttcct
      721 gggccgacag accggtccat tcaaagccaa tgcccggggg cagcttggcg gcgatctctt
      781 ccatggcggc catggccgcg ccggagctct gacccggggc aggggtgccc tggatgttga
      841 ccgatgagag gccgttatag cgctccagac gcggcgagcc gtaggtccag gcggtggtgg
      901 cgaacgccga gaagggcacc atcgcgccgc tgctgttgcg cacgtaccag cggtccaggt
      961 cttcgggctt catgcggaac ggcgcgtcgg cctggacata gaccttcttg atgcggccgc
     1021 gatcgatgaa gtcgttgacg taggaaccgc ccagggccgt gctcagcgcc gcgttgatgt
     1081 cggcggtggt caggcccatg gcgccggccc tggcctgatc gacatcgatg cgcagctgcg
     1141 gggtgtcgtc ctggccgttg gggcgaacgc cggccagcac cgggttctgg gcggccatgc
     1201 ccagcatcat gttgcgggcg ttgatcaggg cctcatggcc cacgccgccg gtgtcctgca
     1261 gctggaagtc gaagcccgag gcgttgccca gctcctgcac cgccggcggc acgatcggga
     1321 agaccatggc gtcgcggatc tgggagaagg cgccgaaggc gcgaccggcg acggccggag
     1381 ccttcaggtc ggcgctcttg cgctcctcga acggcttgag gctgatgaag gccaggcccg
     1441 cgttttgccc tgcgccggcg aagctgaagc cggagacggt gaagaccgac ttgacggcct
     1501 tgtcggccag gaagtggttt tcgacctgct tgagcacgcc ctgggtgcgc tcgtcggtgg
     1561 cgccggccgg cagctggacc agggtgtaga tggtgccctg gtcttcttcc ggcaggaacg
     1621 aggtcggcat ccggatgaac aggaaggcca tgaccacgat gatggcgccg tagacgacca
     1681 tccagcggcc gctcttgccc aggatgccgc gcaccgaacc ctgatagcga cccgacaggt
     1741 cgttgaagct gcggttgaac cagccgaaga agcccttgtn nnnnnnnnng acgggcttga
     1801 gcagggtggc ggtcagggcg ggggtcagca ccagggcgac gaccaccgac agcagcatgg
     1861 ccgagacgat ggtgatcgag aactggcgat agatcacgcc ctgcgagccg ccgaagaagg
     1921 ccatgggcac gaacacggcc gacagcacca gggcgatgcc gatcagggcg ccgctgattt
     1981 ccgtcatcga ctttcgggtg gcctcgaccg gcgacagccc ttcctcgctc atcacccgtt
     2041 cgacgttctc gaccacgact atggcgtcat cgaccagcag gccgatggcc agcaccacgc
     2101 cgaacatggt cagggtgttg atcgagtagc cgaagaccgc caggattccg aaggtgccca
     2161 gcagaaccac cggcacggcg atggtcggga tcagggtcgc gcgccagttc tgcaggaaca
     2221 ggaacatgac gatgaagacc aggatgatgg cttcgaccag ggtcttgacc acctcgtgga
     2281 tcgactgctc gacgaacggc gtggagtcgt aggggaccac atattcgtag ccggccggga
     2341 agctcttgga gagctcggtc atgcgcgcct tcaccgcctc ggcggtgtcc agcgcgttgc
     2401 cgccgggggc cagtttgacg gccaggccgg ccgcgggcgc gccgttgaag tgggcgttga
     2461 agacatagct ttccgcgccc agctcgacgc gggccacgtc acgcaggtgc acgatggcgc
     2521 cgccggtggt gttgcggatg atgatcgccc cgaactgctc gggcgtctgc aggcgcgact
     2581 gggccgagat ggtggcgttc agggccacgc cgggcgcggc gggcgtgccg cccagctggc
     2641 ccgccgagac ctgggcgttc tgggcccgga tggcggcggt gacgtcgctg ggggtcagcg
     2701 agaagctggc cagcttgttg gggtccagcc agatgcgcat ggcatattgc gcgccaaaca
     2761 gctggacgtc gcccacgcct tcgacgcggc tcagcgggtc ctggatgttg gcggcgatat
     2821 agtcggccag gtcgacattg gttgtgcccg gcgccttgga atagaggccg accaccatca
     2881 ggaagttgcg ggtcgacttg accacgctca ggccctgttg ctgcacctct tgcggcagaa
     2941 gcgcggtggc cgtctgcagc ttgttctgga cctgcacctg ggcgatgtcg acgtcggtgc
     3001 cggccttgaa ggtcagggtg gtggtggccg cgcccgagct gtcactggtc gaggacatgt
     3061 attccaggcc gtccaggccc ttcatcttct gctcgatgac ctgggtgacg ctgtcctcga
     3121 cggtcttggc cgaggcgccg ggatagttgg ccgacaccga cacctggggc agggcgatgc
     3181 tgggatactg cgcgatgggc agggtgcgga tggccaggag gccggccagc atgatgacga
     3241 tggcgatgac ccacgcgaag atgggcctgt cgatgaagaa gcgggaaagc atggcggaca
     3301 ggccttagcg ttgggcggtg atgacggcgg gctttacggg cgcgccggga cggaccttct
     3361 gcaggccttc gacgatgacc tggtcgccgg gcttcagccc gctggtcacc agccacttgt
     3421 cgccgaccat ctggcgcacc gtgatcgggc gcggctcggc caggttcttg gcgccgacca
     3481 ccatgacggt ggcgttgccc ttggggtcgc ggctgacggc gggttgcggg atcagcatgc
     3541 cgttctgcgc cgtgaccgtg cccagcaccg cgcggacata catgcccggc agcagcacgc
     3601 cgttggggtt ggcgaaggtg gcgcgcagac ccaccgagcc cgtggtcggg tcgacggtga
     3661 cgtccgagaa ttccagcttg ccgggtgtgg gataggtgga gccgtcctcc agcttcaggg
     3721 tcacctgggc cgaacccgag cggcccacgc tgccgttggt cagatcgcgc tgcagcttca
     3781 aaagttcggc cgaggactgg gtcaggtcga catagacccg gctggtgtcc tgcacggtgg
     3841 ccagggcggc cgcctggctg gcggtgacca gggcgcccgg cgtgaccgag ctcttgccga
     3901 tgcggcccga gattggcgac agcaccctag tgtagcccag attgatgcgg gcggcgtcga
     3961 cggcggcttt ctgggcggcg acattggcgg cggcctgctg gctggcggcc tgggcgtcgt
     4021 cattgtcctg cttgctgacg gcgccggtgg cgaccagcgc gccgaagcgg tcggccttca
     4081 gcttggcggc ggtgtaggcg gcctgggcct gggccaggcc cgcggcggcg ctgttatagg
     4141 cggcctgata ggtggccgga tcgatctggt acagcgcctg gccggcgcgg acatagccgc
     4201 cttcctggaa caggcgggcc ttgacgatgc cgctgacctg cgggcgcact tcgctgacca
     4261 gataggccga ggtccggccc gacagctcgg tggtcaggct gaccggctcg gtcctggcga
     4321 tgaccacgcc cacatctgat ggtccgccca tgccaggcgc gccttcggcg cccttctgcc
     4381 cgcaggcggc cagggtcagc gagatagcgg cgagcacgac ggcggacgcc gtcgaacgtt
     4441 gaaattgcat gtgagacccc ggaggcgccg ggtccgccgg gctcttgccc ttgacgtcgc
     4501 cggtatgatg aacaacatga gaatgaaagt tcattctcat tcgaggctgg acataagtgc
     4561 gaggctgtcg caagtcaaca ccagcagcgc cgcttttcgg ctgacctaac ggaaatgtaa
     4621 tgcgcgacgg gatagtcaat gggtg
//
LOCUS       DAOMEO010000090         4618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2302_length_4618_cov_2.122945, whole genome shotgun sequence.
ACCESSION   DAOMEO010000090 DAOMEO010000000
VERSION     DAOMEO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_4618_cov_2.122945"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>786)
                     /locus_tag="PLH31_03075"
     CDS             complement(<1..>786)
                     /locus_tag="PLH31_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQR88249.1"
                     /translation="EHDDVRNAHLACQEDVLMTNASGAESVVRRCTGEFACPFQRVEH
                     LRHFVSRRAFDIEGLGEKQLQAFFDEGWIREPADIFRLARDAEKLDALRTRDGYGETS
                     VANLVKGIEARRAIGLDRVIYGLGMRDIGETTSTVLARHFDTFADLQAAAEAAATGLP
                     TATYLELSTAPGVGPKALDLLVEVGKGGLKADPWPEADSLEIKIGHAVPKLTKPARAA
                     LTQRYGTWDAFARALAEAANGAPGEDYLHLAAVDGVGPVAAQSL"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<797..>1334)
                     /locus_tag="PLH31_03080"
     CDS             complement(<797..>1334)
                     /locus_tag="PLH31_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQR88250.1"
                     /translation="RFFGYAWGLVSDSFAEGQWEALAKLEAWGFVTTAPPAKRVENAQ
                     GLLDIYAAFETLRPTLDFDIDGVVYKVDDLNLQKRLGFVSRSPRWAIARKFPAQKART
                     ILEAIDLQVGRTGAITPVARLKPVTVGGVSVTNATLHNGDEIARLDVRIGDTVVIQRA
                     GDVIPQIVEVALEARPDPA"
     assembly_gap    1335..1344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1345..1957)
                     /locus_tag="PLH31_03085"
                     /pseudo
     CDS             complement(1345..1957)
                     /locus_tag="PLH31_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516963.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     gene            2064..>3147
                     /locus_tag="PLH31_03090"
     CDS             2064..>3147
                     /locus_tag="PLH31_03090"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR88251.1"
                     /translation="MPKAPQTSRIGSRELSRGGPLDALRFFAAFFMVIYHYAEQAPVS
                     LFSIHPALGRGYLATDFFLMLSGYVLGRAYGDRVTRRQVGDREFLLKRFARVWPSHLV
                     MLSGFVALILATTLVGIAPQNPQWFQWSQLPAQALLIQAWGFDGPSGWNTPTWTLSAL
                     IFCYAAFPVLWRSFAAIRSPWISLAVGVVIFIAVDLACRYWLGQASYRLALKYGVIRA
                     LPLFLIGMLIARMGREVVVSGRAADLLGAAAIAGVVGLQLIGQFDHASLALLGVLIFA
                     AGASARKDWDWAKTAGRLAFALYLTNQFVGVTWFGAQHVAAGKLALNDTALWLTWALA
                     FPAAVVFAALFERFVDTPLHAWVNPRV"
     assembly_gap    3148..3157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3809..>4618)
                     /locus_tag="PLH31_03095"
     CDS             complement(3809..>4618)
                     /locus_tag="PLH31_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQR88252.1"
                     /translation="VAETFEFEPDRLEKTEERLFALRGMARKLNVATENLPSLRAEFA
                     ARLQAIETSGEALKAAEEEAASARDGYVFAAEALSAERRAAGDRLALAVEAELAPLKL
                     EKAKFRVAVEPLPEDRVGPTGMDRVAFEISTNPGAPFGPMETIASGGELARFALALKA
                     ALAGRGTGPQPLMIFDEVDQGVGGAVADAVGLRLKRLAQNAQVLVVTHSAQVAARSDA
                     HWRIAKSGDDQSTRTRVEPLSPAQREEEIARMLSGAEVTEAARAAARALIN"
BASE COUNT          650 a         1543 c         1568 g          827 t
ORIGIN      
        1 agggactgcg cggcgaccgg gccaacgcca tcgacggcgg ccagatgcag atagtcctcg
       61 cccggcgcgc cattggccgc ctcggccagg gcccgggcga aggcgtccca ggttccgtag
      121 cgctgggtca gggcggcgcg ggccggtttg gtcagcttgg ggacggcgtg accgatcttg
      181 atctccaggc tgtcggcctc tggccatgga tcggccttca gcccgccctt gccgacctcg
      241 accaacaggt ccagcgcctt gggcccgacg cccggcgcgg tggaaagctc cagataggta
      301 gcggtgggca ggcccgtggc ggcggcctcg gcggccgcct ggaggtcggc gaaggtgtcg
      361 aagtggcggg ccaacacggt cgaggtcgtc tcgccgatat cgcgcatgcc caggccatag
      421 atcaccctat cgaggccaat ggcgcgccgc gcctcgatgc ccttgaccag attggcgacc
      481 gaggtctcgc catagccgtc acgggtgcgc agggcgtcca gcttttccgc gtcgcgggcc
      541 aggcggaaga tgtcggcggg ctcgcggatc cagccctcgt cgaaaaaggc ctgcagctgc
      601 ttctcgccca gcccctcgat gtcgaaggcc cggcgcgaca cgaagtggcg caggtgctcc
      661 acccgctgga acggacaggc gaactctccc gtacagcggc gcaccaccga ctcggccccc
      721 gaggcgttgg tcatgagcac atcctcttgg cacgccaagt gggcgttcct tacatcgtcg
      781 tgttccnnnn nnnnnngcgc gggatcgggc cgcgcctcca gcgccacctc gacgatctgc
      841 gggatcacgt cgccggcccg ctggatcacc accgtgtcgc caatccgtac gtccagccgg
      901 gcgatctcgt cgccattgtg cagggtggcg ttggtcaccg agacgccgcc caccgtcacc
      961 ggcttcagcc gcgccaccgg ggtgatcgcg ccggtgcggc ccacctgcag gtcgatggcc
     1021 tccaggatcg tccgagcctt ctgggccgga aacttgcggg cgatggccca gcggggcgag
     1081 cgcgagacga aacccaggcg cttttgcaaa ttcaggtcgt cgaccttgta gacgacgccg
     1141 tcgatgtcga aatccagggt cggtcgcagg gtctcgaagg cggcatagat gtcgagcagg
     1201 ccttgcgcgt tctccacccg tttggccggc ggggccgtgg tgacgaaacc ccacgcctcc
     1261 aacttggcaa gagcctccca ctggccctcg gcgaagctgt cgctgaccag gccccaggca
     1321 tagccgaaga accgnnnnnn nnnnggccgg ccgcctcggc cgccgcgttg aacgcggcga
     1381 aggcggccag ctcgacatag acctcgccgc gcacctcgat cacctcgggc caaccggagc
     1441 ctttgaggcg atgggggatg tcggcgatgg tgcgcagatt ggcggtgacg tcctcgccca
     1501 cccggccgtc gccccgcgtc gcgccctgga ccagcacgcc cttttcatag cgcaatgagg
     1561 ccgacagacc gtcgatcttg ggctcggccg tataggccac ggtctcgtcg ggcgacagct
     1621 tcaggaaccg ccggatgcgg gcgtcgaact cgatcgcctc gtcattggaa aaggcgttgt
     1681 cgaggctcag catcggcacg ccgtgctcga ccggcgagaa ctggtcggcc cgcgccgcgc
     1741 ccacgctgac ggacggcgag ttctcgcgga tcaggtgcgg aaagcgggtc tctatgtcga
     1801 ggttgcggcg cttcagagcg tcgtactcgg cgtcgctgat cgtgggcgcg tcctgctggt
     1861 gatagcgcag gtcatgggcc gccagctgat cggccagcgc ctccagttcc agggcggcct
     1921 gggcttcgga aaggtcagcg acgggcgtca gggtcacggg cgaatccggc tgggaaggtc
     1981 aggtcgtctt tttaaccgat gccggggcaa aacgctcctg ctggcgcgcg gcttgctgca
     2041 gcggcgcgag acaggagccc gtgatgccga aagccccgca aacgtcccgt attggctcgc
     2101 gcgagctttc tcgtggcggt cccctggacg cccttcggtt cttcgcggcc ttcttcatgg
     2161 tgatctatca ctatgccgaa caggcgccgg tcagcctgtt ctcgatccat ccggcgctgg
     2221 gacgcggcta tctggctacc gacttcttcc tgatgctgtc gggctatgtg ctgggccgcg
     2281 cctatggcga ccgggtcacc cggcgccagg ttggcgaccg cgagttcctg ctcaagcgtt
     2341 tcgcccgcgt ctggccctcg catctggtga tgctgagcgg ctttgtcgct ctgatcctcg
     2401 ccaccaccct ggtcggcatc gcgccgcaga acccgcagtg gttccagtgg tcgcagctgc
     2461 cggcccaggc gctgctgatc caggcctggg gcttcgacgg cccgtccggc tggaacactc
     2521 cgacctggac cctgtcggcc ctgatcttct gctacgccgc cttcccggtg ctgtggcgca
     2581 gctttgccgc cattcgctcg ccgtggatct ccctggccgt tggcgtggtg atcttcatcg
     2641 cggtggatct ggcctgccgc tactggctgg gtcaggcgtc ctatcgcctg gcgctgaagt
     2701 acggcgtgat ccgcgccctg cccctgtttc tgatcggcat gctgatcgcc cgaatgggtc
     2761 gcgaagtggt ggtcagcggc cgcgcggccg acctgctggg cgcggcggct atcgccggtg
     2821 tcgtcggcct gcaattgatc gggcaatttg accatgccag cctggccctg ctgggcgtgc
     2881 tgatctttgc ggccggcgct tcggcccgga aggactggga ctgggcgaag acggcggggc
     2941 ggctggcctt cgccctctac ctgaccaacc agttcgtggg cgtgacctgg tttggcgccc
     3001 agcacgtggc ggccggcaag ctcgccttaa acgataccgc cctgtggctg acctgggcct
     3061 tggccttccc ggccgccgtg gtcttcgccg ccctgtttga gcgtttcgtg gacaccccgc
     3121 tgcacgcctg ggtcaatccg cgcgtcgnnn nnnnnnncga gatcagcgcc cggcgggcga
     3181 tgttccgcgt caacccggcg atgtgggcca agcggcgggt ggtctgaggg ctattgggga
     3241 ttcgccgtct tgcggggcgt tcgctgggcg tgagactggc tcgccatagg gcttttgaag
     3301 gtctggaatc ggtcacagag ccaaagcttg cttccgaccc tgagcggaca tgccgagacg
     3361 cgaccggagg cttgggctac gcttgagtca tgacccgtcg cagatttagt gcgctccgta
     3421 cttggtggat gtggcacaaa caaaccgctg ggctagtgct cgccgctggc gctttggttt
     3481 ccattgtctt gctcgtcatg ccgttcaccg gtgaaactgc cttggaaggc actgtaaccg
     3541 acgttcgata tctaggctca aaaggcgctg tgacgacata cgccgttgtc gagacgtccg
     3601 gacaacgggc gatggtgagg ctagatggcg tgcatggctg tcatgtcggg agccccattc
     3661 cgcttcagaa gcgactgtct ctgacgggcg ctagatatac cgcatcactc ggctgtcacc
     3721 gaattgctgc tccccgtgct cctgcccgct aaccaccctt ctcagccgtt gggatggtcc
     3781 gctttcggcg cgttcggctc gacgctgcct agttgatcag agcccgcgcc gccgccctcg
     3841 ccgcctcggt gacttccgcc cccgacagca tccgcgcgat ctcttcctcg cgctgagccg
     3901 gcgatagcgg ctcgacgcgc gtgcgggtgg actgatcgtc gccggacttg gcaatgcgcc
     3961 agtgggcgtc actgcgggct gccacctggg ccgagtgggt gaccaccagg acctgggcgt
     4021 tctgggccag gcgcttcagg cgcagaccca ccgcgtcggc tacggcgccg ccgacgccct
     4081 gatcgacctc gtcgaagatc atcagaggct gcgggccggt tccgcgaccc gccagagccg
     4141 ccttcagcgc cagagcgaaa cgggccagtt cgccgcccga ggcgatggtc tccatggggc
     4201 cgaacggcgc gccgggattg gtcgagatct cgaaggcgac gcggtccatg ccggtggggc
     4261 cgacgcggtc ctcgggcagg ggctcgacgg cgacgcggaa cttggccttt tccagcttca
     4321 gcggcgccag ttcggcctcg acggccaggg ccagccggtc gccggcggcg cggcgttcgg
     4381 ccgacagggc ctcggcggcg aagacatagc cgtcgcgggc cgaggccgcc tcttcctcgg
     4441 cggccttcag ggcctcgcca gaggtctcga tggcctgcag acgggccgcg aactcggcgc
     4501 gcagggacgg caggttctcg gtggcgacgt tcagcttgcg ggccatgccg cgcagggcga
     4561 acaatcgctc ctcggtcttt tccagacggt cgggttcgaa ttcgaaggtt tcggcaac
//
LOCUS       DAOMEO010000091         4592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2316_length_4592_cov_2.825656, whole genome shotgun sequence.
ACCESSION   DAOMEO010000091 DAOMEO010000000
VERSION     DAOMEO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2316_length_4592_cov_2.825656"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLH31_03100"
     CDS             complement(<1..708)
                     /locus_tag="PLH31_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HQR88253.1"
                     /translation="MELECYPTENRPPEIVPGRPQRAWMDHFADRHPYRCLPLSMANT
                     SGWEILCPVGFSMTWDGGAHQDCIKFQPDHPYPGFTEFVKSHFSRGTVTFHTGYLFRT
                     PPGWSILTMGPPNHIKDGIQPLAGLVETDWLPFPFTMNWLFTRPGTVRFEKGEPFCFI
                     MMMQDKVMDNVQPVIRSMNSNLDLRRQYDVWAEKRSEFNSLIFKRDPEATKEAWQRFY
                     FKGEYPEEVQAEAPSHHV"
     gene            812..1318
                     /locus_tag="PLH31_03105"
     CDS             812..1318
                     /locus_tag="PLH31_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HQR88254.1"
                     /translation="MKTRADLFAFLDAHGIAHSTLDHPPVFRVEEGLEIKAALPGGHT
                     KNLFLKDARGQLWLISALGETVIDLKRLHHVIGSGRLSFGPEAMMVETLGVTPGSVTA
                     LGLINDTDRRIRFVLDAALAKADPVNFHPLSNNATTALSQDGLRTFLAALGITPMIVD
                     FEAMAVVS"
     gene            1396..2316
                     /locus_tag="PLH31_03110"
     CDS             1396..2316
                     /locus_tag="PLH31_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HQR88255.1"
                     /translation="MNLIGDKPAPIQAGGPNGGKSAHIKDGTDASFMADVIEASKTQP
                     VIVDFWATWCGPCRQLTPALEKAVTAAGGAVKLVKIDVDKNPGYSGQLRVQSIPTVYA
                     FVNGQPVDGFQGAVPESEIKAFIARISGPPAQSDLDALLAMARESVEIGDIGGAAQAY
                     AQALQMDPANVKAIGGMARCYLDSGDLEGAAATAAMAPANAKDADLESVRAALALAAD
                     APSETATFEARLAADPNDHEARFELAKALAGAGHLQAAADHLLTIIERDRTWNDEAAR
                     KQLLTVFEAAGAMSEVAKAGRKRLSSILFS"
     gene            2328..2996
                     /locus_tag="PLH31_03115"
     CDS             2328..2996
                     /locus_tag="PLH31_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQR88256.1"
                     /translation="MPGIRKLSDLPLVIPVFPLDGVLLLPGGQLPLNIFEPRYLNMLD
                     DVMSGERMIGIIQTRPGNAGPDDEHNPGLAPIGCAGRVTSFAETSDGRYLITLTGVCR
                     FRAGDELPVRTPYRQVRADFSSFAADLREDSAEPAADPGPLLAALRRYLDHRGLAIDW
                     TSAETAPSDALINSLSMALPFDAMEKQALLEAPTMADRRETLVALLEIDAVVGDDDEP
                     PSIQ"
     gene            3040..3246
                     /locus_tag="PLH31_03120"
     CDS             3040..3246
                     /locus_tag="PLH31_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQR88257.1"
                     /translation="MTATPTPPPADIDPRLLEVLVCPVTRTTLDYNRVRGELVSRAAK
                     LAYPIRDGVPIMLPEEARSLEDGE"
     gene            3251..>3550
                     /locus_tag="PLH31_03125"
     CDS             3251..>3550
                     /locus_tag="PLH31_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQR88258.1"
                     /translation="MAFDAGLAKPPRLASLDVLRGLTIVGMILVNSAAYLHYVSGYDV
                     FPVLMHARWAGFTLADAVFPAFIVMTGVSIPLATAERGLDGAGLLRLLVRTGRLIL"
     assembly_gap    3551..3560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3561..4349
                     /locus_tag="PLH31_03130"
     CDS             <3561..4349
                     /locus_tag="PLH31_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88259.1"
                     /translation="VSNLYWLATPESVVFRPMGVLQRIALAFFAAAVLYRLTGPRTRL
                     TIAVVLLLLYWPLCLLPYPGGAADLTQPGASLIAYADRLVLGVHTFVKGPLGYDPEGL
                     LGTLPAIAQALIGVAAGEWLAARRGTRGLAIAGALLLVVGIAWGLAFPVVKDLWTSSY
                     VLVSSGATLLVLAALHQALDIKLWRVPGRSFLTDFGVNAIFAYVLHQLASIMLTAHML
                     KIPYDAASPVVGTRTAALIPVAIFIGLIWAVVAYMRKRNWIVKV"
     gene            complement(4472..>4592)
                     /locus_tag="PLH31_03135"
     CDS             complement(4472..>4592)
                     /locus_tag="PLH31_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88260.1"
                     /translation="ARGIGLAAVNRIAPARRFFMHEAGGAVGDLPRLLRGHAI"
BASE COUNT          712 a         1597 c         1498 g          775 t
ORIGIN      
        1 gacatggtgc gacggcgcct cggcctgcac ttcctcggga tactcgccct tgaagtagaa
       61 gcgctgccag gcctccttgg tggcctcggg gtcgcgcttg aagatcaggc tgttgaactc
      121 gctgcgcttc tcggcccaga cgtcgtactg gcggcgcaaa tccaggtttg agttcatcga
      181 gcggatcacc ggctggacgt tatccatcac cttgtcctgc atcatcatga tgaaacagaa
      241 cggctcgccc ttttcgaagc gcaccgtgcc ggggcgggtg aacagccagt tcatcgtgaa
      301 ggggaagggc agccagtcgg tttcgaccag gccggcgagg ggctggatgc catccttgat
      361 gtggttgggc ggccccatgg tcaggatcga ccagcccggc ggcgtgcgga acagatagcc
      421 cgtatggaaa gtcaccgtgc cgcgcgagaa gtgcgacttc acgaactcgg taaagcccgg
      481 ataggggtgg tcgggctgga acttgatgca gtcctggtgc gcgccgccgt cccaggtcat
      541 cgagaagccg accgggcaca ggatctccca gcccgaggtg ttggccatcg acagcggcag
      601 gcagcgatag gggtgacggt cggcgaagtg gtccatccag gcccgctgcg gacggcccgg
      661 cacgatctct ggcgggcggt tctcggtggg atagcactcc agttccatgc cgcgcgtctt
      721 cctgttttcc ttgacccttg gctctggtct agcgcccgcg aggcgaggcc gtccagttcc
      781 ccgaacagca aggctcggct aggaagccct gatgaagacc cgagccgacc ttttcgcctt
      841 cctcgacgcc cacggcatcg cccattccac cctcgatcat ccgcccgtct tccgcgtgga
      901 ggaagggctg gagatcaagg cggccctgcc gggcggccat accaagaacc tgttcctgaa
      961 ggacgccagg gggcagctgt ggctgatctc ggcgctcggc gagacggtga tcgatctcaa
     1021 gcgcctgcac cacgtgatcg gctcgggccg gctttcgttc gggccagaag ccatgatggt
     1081 cgagacgctg ggcgtgaccc cgggctcggt gacggccctg gggctgatca acgacaccga
     1141 ccgccgcatc cgcttcgtgc tggacgcggc cctggccaag gccgatccgg tcaacttcca
     1201 ccccttgagc aacaacgcga ccacggcgct cagtcaggac ggcctccgaa cattcctggc
     1261 ggcgctgggg atcacgccga tgatcgtcga tttcgaggcc atggcagtcg tctcttgagc
     1321 ccccccgatt gcggttgtcg ccttgaggga ccatcttgcc cctgaccgcg ttcctgcctg
     1381 tatgagagac ttccgatgaa cctgatcggc gacaagcccg cccctattca agctggcggc
     1441 ccgaatggcg gcaagagcgc gcatatcaag gacggcacag acgccagctt catggctgac
     1501 gtcatcgagg cgtccaagac ccagcccgtc atcgtcgact tctgggccac ctggtgcggc
     1561 ccctgtcgcc agctgacccc ggcgctggaa aaggccgtca ccgccgccgg cggcgcggtc
     1621 aagctggtca agatcgacgt cgacaagaac cccggctatt cgggccagct gcgcgtgcag
     1681 tcgatcccca ccgtctacgc cttcgtcaac ggccagccgg tggacggctt ccagggcgcg
     1741 gtgcccgaga gcgagatcaa ggccttcatc gcccgtatct ccggcccgcc ggcccagagc
     1801 gatctggacg ccctgctggc catggcccgg gagtcggtcg agatcggcga tatcggcggc
     1861 gcggcccagg cctacgccca ggccctgcag atggatccgg ccaacgtgaa ggccatcggc
     1921 ggcatggccc gctgttatct cgacagcggc gaccttgaag gcgccgccgc aacagccgca
     1981 atggcgccgg ccaacgccaa ggacgccgat ctggaaagcg tccgcgccgc cctggccctg
     2041 gccgccgacg cgccgtccga gacggcgacg ttcgaggcgc gtctggccgc cgatcccaac
     2101 gaccacgaag cccgcttcga gctggccaag gccctggccg gcgcgggcca tctgcaggcc
     2161 gccgccgatc acctgctgac catcatcgag cgcgaccgca cctggaatga cgaggcggcg
     2221 cgcaagcagc tgctcaccgt gttcgaggcg gccggcgcga tgtcggaagt cgccaaggcc
     2281 ggccgcaagc ggctgtcctc catcctgttc agctagaagg acctccgatg ccgggtattc
     2341 gaaagctcag cgacctgccg ctggtgatcc cggtattccc gctggacggg gtgctgttgc
     2401 tgcccggtgg gcagctgccg ctcaacatct tcgagccgcg ctatctgaac atgctggacg
     2461 acgtgatgtc cggcgagcgg atgatcggca tcattcagac ccggcccggt aacgcgggcc
     2521 ccgacgacga gcacaatccc ggcctggcgc cgatcggctg cgccggccgg gtgaccagtt
     2581 ttgccgagac cagcgatggc cgctacctga tcaccctgac cggggtctgc cggttccggg
     2641 ccggcgacga gctgccggtg cgcacgccgt atcgccaggt gcgcgctgac ttctcgtcgt
     2701 tcgcggctga cctgcgcgaa gatagcgccg agcctgccgc cgacccgggc ccgctgctgg
     2761 ccgcactgcg tcgctatctg gaccacaggg gcctggccat cgactggacc agcgccgaga
     2821 ccgccccctc ggacgccctg atcaacagcc tgtcgatggc cctgcccttc gacgccatgg
     2881 aaaaacaggc cttgctggag gccccgacca tggccgaccg gcgcgagact ctggtggcgc
     2941 tgctggagat cgatgcggtc gtgggcgacg acgacgagcc cccttcgatc cagtaagcgc
     3001 cccctatcca gtcggaatgt ggggcggtag agagatttca tgaccgcgac cccgactccg
     3061 ccgcccgccg acatcgaccc gcgcctgctg gaggtgctgg tctgtcccgt cacccgcacg
     3121 acgctggact acaaccgcgt gcgcggcgaa ctggtcagcc gggccgccaa actggcctat
     3181 ccgatccgcg acggcgtgcc gatcatgttg ccggaagagg ctcgctcgct cgaagacggc
     3241 gaataggtcc gtggccttcg acgctggcct ggccaagccg ccacgcctgg cgtcgctgga
     3301 tgtgctgcga ggcctgacca tcgtcggcat gatcctcgtc aattcggccg cctacctgca
     3361 ttacgtcagc ggctacgacg tttttccggt gttgatgcac gcgcgctggg ccggcttcac
     3421 cctggccgac gcggtgttcc ccgccttcat cgtcatgacc ggcgtctcga tcccgctggc
     3481 caccgccgag cgcggcctgg acggcgcggg cctgcttcgc ctgctggtgc ggaccggacg
     3541 gctgatcctg nnnnnnnnnn gtcagcaacc tttactggct ggccacgccc gagagcgtcg
     3601 tcttccggcc gatgggcgtg ctgcagcgca tcgccctggc cttcttcgcc gccgcagtcc
     3661 tgtaccgcct caccgggccc cgcacgcgcc tgacgatcgc ggtggtcctg ctgctgctct
     3721 actggccgct gtgtctactg ccctatcccg gcggcgcagc cgacctgacc cagcccgggg
     3781 ccagcctgat cgcctatgcc gatcgcctgg tgctgggcgt ccataccttc gtcaagggcc
     3841 cgctgggcta tgaccccgag ggtttgcttg gaaccctgcc cgccatcgcc caggccctga
     3901 tcggcgtggc ggccggcgaa tggctagcag cccggcgcgg cacccgaggc ctggcgatcg
     3961 cgggcgccct gctgctggtg gtcgggatcg cctggggctt agccttcccg gtcgtgaagg
     4021 acctgtggac cagcagctat gttctggtct ccagcggcgc gaccctgctg gtgctggcgg
     4081 ccctgcacca ggcgctggac atcaagctat ggcgcgtgcc ggggcgaagc ttcctgaccg
     4141 atttcggggt caacgccatc ttcgcctatg tgctgcacca gctggcctcg atcatgctga
     4201 ccgcgcacat gctgaagatc ccctatgacg cggcctcgcc ggtggtgggg accaggacgg
     4261 cggccctgat cccggtcgcg atcttcatcg gcctgatctg ggcggtggtc gcctacatgc
     4321 gcaagcggaa ctggatcgtg aaggtttagg tccaaagtcc tccccctgaa gggggaggcg
     4381 gtcgcatagc gaccggaggg ggaagtaccc tctgttcatg gcagcccctc tccgccggct
     4441 tcgccgaccc ctctcccaca gggagaggac ttcagatcgc gtgccccctg agcagcctgg
     4501 gcaggtcgcc cactgcgccg ccggcctcat gcatgaagaa ccgtcgcgcc ggggcaatgc
     4561 ggttgaccgc cgccaggccg atgccccgcg cc
//
LOCUS       DAOMEO010000092         4584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2321_length_4584_cov_2.822477, whole genome shotgun sequence.
ACCESSION   DAOMEO010000092 DAOMEO010000000
VERSION     DAOMEO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_4584_cov_2.822477"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLH31_03140"
     CDS             <1..799
                     /locus_tag="PLH31_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQR88261.1"
                     /translation="VAVACGWDAFEGFLAGGAAMDAHFASAPLDRNAPVLLALAQIFN
                     RNGLDRRARSVVPYSHRLRRLASFLQQLEMESNGKSVGPDGQPAKRGTATVVFGDEGT
                     NVQHAYFQCMHQGTDITPMELIGLAKSDEGPAGMHEKLLSNLLAQAEAFMVGRTTDDV
                     RAELVAKGVSDAEIATLAPQRTFAGNRPSTLLLLERLTPQTFGALIALYEHKTFVEGV
                     IWGINSFDQWGVELGKVMAGRILPELESGAVGRHDPSTTALIGRLKV"
     gene            complement(803..1792)
                     /locus_tag="PLH31_03145"
     CDS             complement(803..1792)
                     /locus_tag="PLH31_03145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HQR88262.1"
                     /translation="MPAPFPPPFRGHEVEARLRPAAEAYPFDLDRVLASVVALESRVP
                     SDAYTANILGTERIGNGVVISPTGLVLTMAYLVTEAREVILTLNDGRRVSGHVLGFDP
                     RSGLGLVQALSPLDVPVMPIGTAKGLTAGDAVIAAGAGGRAHAAAGQILTRMPFSGYW
                     EYHLDEAIMTEPAHPHWSGAALIGPKGDLVGLGYLSLEARAADGSPRPINMFVPAELL
                     PPILDDLARGQPPHPPRPWLGVFTQEIGDHVVIVGISPKGPAARAELKPGDIILAVDG
                     QGVADLAEFYNALWSQGPAGATIPLQVLREGDTFEVEVRSMDRGTLLKKPKFN"
     gene            complement(1884..2600)
                     /gene="rph"
                     /locus_tag="PLH31_03150"
     CDS             complement(1884..2600)
                     /gene="rph"
                     /locus_tag="PLH31_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQR88263.1"
                     /translation="MRPSKRAPDQLRAVTLETGVNRYAEGSCLIGFGHTKVLVTASVE
                     ESIPGWMRGKGQGWVTAEYGMLPRATHTRGRREAAAGKQTGRTQEIQRLIGRSLRAVV
                     DMKALGERQISLDCDVIQADGGTRTAAITGAWVALRLATQYLLDEGVLKTDPIIGQVA
                     AVSCGVFADTPVLDLDYEEDSNAEADSNFVLTENGDIVEIQATGEKRGFTRAEFEALY
                     GLAEKGIGELFAFQMAAIGK"
     gene            2711..3775
                     /gene="hrcA"
                     /locus_tag="PLH31_03155"
     CDS             2711..3775
                     /gene="hrcA"
                     /locus_tag="PLH31_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQR88264.1"
                     /translation="MTQLFGRTPGLTELDARARDIFKRVVEAYLETGEPVGSRTLSRG
                     GMSLSPASIRNTMQDLAQLGLLDSPHTSAGRMPTHAGLRMFVDGFLEVGDVGQEERQA
                     IEARLFSRGRSFEEALAEAGSILSGLAGGASIVVTPVREGGVKHVEFVALGGDQALAV
                     MVFEDGTVENRLMKRAAGMTPSAFQEASNFLNSRLRGRTLVEAKDEVRAELEAARRQL
                     DETAARLVEDGLAAWGGGEGDARALIVRGQANLLGEPGAREDLERVRMLFDDLEQKGQ
                     LIGLLDDVREAQGVRIFIGAETRLFSLSGSSVIAAPYMTGQRKVLGAIGVIGPTRLNY
                     ARVIPLVDYTARVLGRMMDG"
     gene            3786..4421
                     /gene="grpE"
                     /locus_tag="PLH31_03160"
     CDS             3786..4421
                     /gene="grpE"
                     /locus_tag="PLH31_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQR88265.1"
                     /translation="MSDDQAPVEEMGMAPEAEDAAQEIEALKLEVAALKDQVFRYAAE
                     AENTKRRAEREMNDARAYAIQKFARDLLGAADNLSRATAMSPRDSQDPAVTNYIIGVE
                     MTEKELLAAFERNGLKKIEPAKGEKFDPHLHQAVMEQPSTEVAAGGVIQFLQAGYELM
                     GRLIRPAMVAVAAKGSTAPGSAEPSSGGGSNPYAGANTEADGSGGSVDIKS"
     gene            complement(4430..4582)
                     /locus_tag="PLH31_03165"
                     /pseudo
     CDS             complement(4430..4582)
                     /locus_tag="PLH31_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
BASE COUNT          703 a         1527 c         1652 g          702 t
ORIGIN      
        1 ggtggccgtg gcctgcggct gggacgcctt cgagggcttc ctggccggcg gcgccgcgat
       61 ggacgcccac ttcgccagcg cgccgctgga caggaacgcc ccggtgctgc tggccctggc
      121 ccagatcttc aaccgcaatg gcctggaccg ccgggcccgc tcagttgttc cgtactcgca
      181 ccgcctgcgc cgcctggcct cgttcctcca gcagctggag atggagagta acggcaagtc
      241 ggtgggcccc gacggccagc cggccaagcg cggcacggcc acggtggtgt tcggcgacga
      301 gggcaccaac gtccagcacg cctatttcca gtgcatgcac caggggaccg acatcacgcc
      361 gatggagctg atcggtctcg ccaaatccga cgaaggcccg gctggcatgc atgaaaagct
      421 gctgtccaac ctgctggccc aggccgaggc cttcatggtg ggccgcacca ccgacgacgt
      481 gcgcgccgag ctggtggcca agggcgtctc ggacgccgaa atcgccaccc tggccccgca
      541 gcgcaccttc gccggcaacc ggccctcgac cctgctgctg ctggagcgcc tgacgccgca
      601 gaccttcggc gccctgatcg ccctctatga gcacaagacc ttcgtcgagg gcgtgatctg
      661 ggggatcaac agcttcgacc agtggggcgt ggagctgggc aaggtgatgg ccggccggat
      721 cctgccggag ctggagagcg gcgcggtggg ccgccacgat ccgtcgacga cggcgctgat
      781 cgggcggctg aaggtttagg cctcagttga acttcggctt cttcagcagc gtcccgcgat
      841 ccatcgagcg gacctcgacc tcgaaggtgt cgccctcgcg cagcacctgc agcggaatgg
      901 tcgcgccggc cggtccctgg gaccacaggg cgttgtagaa ctcggccagg tcggcgacgc
      961 cctggccgtc gacggccagg atgatgtcgc cgggcttcag ttcggcgcgg gccgccgggc
     1021 ccttgggcga gatgccgacg atcaccacgt ggtcgccgat ctcctgggtg aacaccccca
     1081 gccacggtcg cggcggatgc ggcggctggc cgcgcgccag gtcgtcgagg atgggcggca
     1141 gcagttcggc gggcacgaac atgttgatcg gccggggcga gccatcggcg gcgcgggctt
     1201 ccaggctcag atagcccagg ccgaccagat cgcccttggg accgatcagg gccgcgccgc
     1261 tccagtgcgg gtgggccggc tcggtcatga tggcttcgtc cagatggtac tcccaatagc
     1321 ccgagaacgg catccgggtc aggatctggc cggccgcggc atgggcgcgt ccgccggcgc
     1381 cggcggcgat caccgcgtcg cccgccgtca gccccttggc cgtgccgatc ggcatgaccg
     1441 gcacatccag cggcgacagc gcctggacca ggcccagccc gctgcgcgga tcaaagccca
     1501 gcacatggcc cgagacgcgg cggccgtcat tgagggtcag gatcacctct cgcgcctcgg
     1561 tcaccagata ggccatggtc aggaccagcc ccgtggggct gatcacaacg ccgttgccga
     1621 tgcgctccgt gcccaggata ttggccgtgt aggcgtcgga cggcacgcgc gattccaggg
     1681 cgaccaccga ggccagcacc cggtccaggt cgaacggata ggcttcggcg gcggggcgta
     1741 agcgcgcttc gacttcgtgg ccccgaaaag gagggggaaa gggtgcgggc acttggatct
     1801 cctctcggaa atcgaatctg gggctcgtcg acgccccggg caagggggcg gggcgtcgcg
     1861 ggccgcaata gccgtgtggg cgcctacttc ccgatagccg ccatctggaa cgcgaacagc
     1921 tcgccgatgc ccttttccgc caggccgtac agggcctcga actcggcgcg ggtgaagccg
     1981 cgcttttcgc cggtggcctg gatctcgacg atgtcgccgt tctcggtcag gacgaaattg
     2041 ctgtcggcct cggcgttgga gtcttcctca tagtcgaggt ccagcaccgg ggtgtcggcg
     2101 aacacaccgc acgagacggc ggcgacctgg ccgatgatcg ggtcggtctt cagcacgccc
     2161 tcgtccagca gatactgcgt ggccaggcgc agggccaccc aggcgccggt gatcgcggcg
     2221 gtgcgggtgc cgccgtcggc ctggatgacg tcgcagtcca ggctgatctg gcgctcgccc
     2281 agggccttca tgtcgaccac ggcgcgcagg gagcggccga tcaggcgctg gatttcctgg
     2341 gtgcggccgg tctgtttgcc ggcggcggcc tcgcggcggc cgcgggtgtg ggtggcgcgg
     2401 ggcagcatgc cgtactcggc cgtgacccag ccctggccct tgccgcgcat ccagccgggg
     2461 atgctttcct cgacgctggc ggtgaccagc accttggtgt ggccaaagcc gatcaggcac
     2521 gagccctcgg catagcggtt gacgccggtc tccagcgtga cggcgcgcaa ttggtcgggg
     2581 gcgcgtttcg aaggacgcat gggaaactcc ggctttgact gtgagaaggg gccgcttatc
     2641 ctatctgcga cgggaacttg aaagagcgcc cggcgctttt ctgtctcagc gaggtctccg
     2701 gcttaacgcc atgacgcaat tgtttggtcg cacccccggt ctgacggagt tggacgcgcg
     2761 ggcccgcgac atcttcaagc gggtggtcga agcctatctg gagaccggcg agcccgtggg
     2821 gtcgcgcacc ctctcgcggg gcgggatgtc actgtcgccg gcctcgatcc gcaacaccat
     2881 gcaggacctg gcgcagctgg gcctgctgga ttcgccccat accagcgctg gccggatgcc
     2941 cacccatgcg ggcctgcgga tgttcgtcga cggctttctg gaggtcggcg atgtcggcca
     3001 ggaggagcgc caggccatcg aggcccggct gttctcccgc ggccggtcgt tcgaagaggc
     3061 cctggccgag gccggctcga tcctgtcggg tctggccggc ggggccagca tcgtggtcac
     3121 gccggtgcgc gagggcgggg tcaagcatgt cgagttcgtg gccctgggcg gcgaccaggc
     3181 cctggcggtt atggtcttcg aggacggcac ggtcgagaac cggctgatga agcgcgcggc
     3241 cgggatgacg ccctcggcct tccaggaggc gtccaacttc ctcaacagcc gcctgcgggg
     3301 ccgcaccctg gtcgaggcca aggacgaggt ccgggccgag ctggaggccg cccgccggca
     3361 gctggacgag acggcggcgc ggctggtcga ggatggtctg gcggcctggg gcggcggcga
     3421 gggcgacgcc cgggccctga tcgtgcgcgg ccaggccaac ctgctgggcg agcccggggc
     3481 ccgcgaggat ctggagcgcg tccggatgct gtttgacgat ctggagcaga agggtcagct
     3541 gatcggcctg ctcgacgacg tccgcgaagc ccagggcgtg cgcattttca tcggggccga
     3601 aacgcgactc ttttcgcttt cgggttcctc cgtgatcgcg gcgccctata tgacgggcca
     3661 acgcaaggtg ctgggcgcga tcggggtgat cggccccacc cgcctcaact atgcccgggt
     3721 gatcccgctg gtggactata ccgcccgcgt gctcggccgg atgatggatg gataaggact
     3781 aggctatgag tgacgatcaa gcgccggttg aagagatggg catggctccg gaagccgagg
     3841 acgccgccca ggaaatcgag gcgctgaaac tggaagtggc ggccctgaag gatcaggttt
     3901 tccgctacgc cgccgaagcg gaaaacacca agcgccgggc cgagcgcgag atgaacgacg
     3961 cccgcgccta tgcgatccag aagttcgccc gcgacctgct gggcgccgcc gacaacctgt
     4021 cgcgcgccac ggccatgagc ccgcgcgaca gccaggatcc ggcggtgacc aactatatca
     4081 tcggcgttga aatgaccgag aaggagcttc tggcggcctt cgagcggaat ggtctgaaga
     4141 agatcgagcc ggccaagggc gagaagttcg atccccacct gcaccaggcg gtgatggagc
     4201 agccctcgac cgaggtcgcg gccggcggcg tcatccagtt cctgcaggcc ggctacgagc
     4261 tgatgggccg cctgatccgt ccggccatgg tcgccgtggc ggccaagggc tcgaccgcgc
     4321 ccggctcggc cgagccctcg tcgggcggcg gttcaaaccc ctatgcgggc gccaacaccg
     4381 aggccgacgg ctcgggcggt tcggtcgaca tcaagtcctg acggcgcttc tggtcgcggc
     4441 gcctgtcggc ccgcgaccag gcccgttcgt gcaggttgaa gaacaccgtc tggaccagcg
     4501 gctccacgat gccgacggcc agggccacgc gccagtcgcg ggtcagggca taggcgacgg
     4561 cgaccgccac ggtcaggtgc atgg
//
LOCUS       DAOMEO010000093         4572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2324_length_4572_cov_2.292894, whole genome shotgun sequence.
ACCESSION   DAOMEO010000093 DAOMEO010000000
VERSION     DAOMEO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2324_length_4572_cov_2.292894"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2011
                     /locus_tag="PLH31_03170"
     CDS             <1..2011
                     /locus_tag="PLH31_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE2 and HATPase_c domain-containing protein"
                     /protein_id="HQR88266.1"
                     /translation="DHLTAAGARTIVYDVLFSQPGANPGDDVALASSIALSGRVYLPQ
                     FIEPGFGEGAPQQVVGPLPEIARGAAGLGSAHVLFDADGVVRRMEPFQAFGPDLTPDL
                     MTVVHRATRAFDTRDITRGAETTLLIPFAGAPNRFPRVSFSSVLAGDIPSELLAGRTV
                     FVGMTAPGLGPAFPTPVTPDAASMTGVQLQASILDGLRSGTMIRPAGLMSRIAFALSL
                     LWLLLAGLLVLPPRANLMLVVGLSSLALALSAAFFWSLDLWLSPVATIIGLATVSLVW
                     GWGQLGRVSDLLGHELRRLLPVAQTPPAEGFESDLVARQALSLGAAINRIDDLRRFSA
                     DALFSLPDATVVLDARRRVVAANAAAQTLLRATHLPLAGASVASLLNALEPLGRTFDE
                     SWPPEAERIEPVDLHLSDGRAYQSQTVFRRDEAGESAGWIVRLADITTLTAAMRQREQ
                     ALQLLTHDMRSPQTSILALLATTPDLPEDVSDRLAACARRTLALADGFVQLARAEVTP
                     VAMEPVDLCDVAVEAVDELWPQCRQRHIEIEQEGCDAPLMVLGDRSVLARALVNLLGN
                     AVKYSPEGSRIRVSARATREDGRAFVDVSISDHGPGLSPEEAAMAFRAFQRFDRPGSE
                     AAGGVGLGLAFVDAAAARHGGGVLCASAPGAGATFTLRLPEYRPA"
     gene            complement(2072..2533)
                     /locus_tag="PLH31_03175"
     CDS             complement(2072..2533)
                     /locus_tag="PLH31_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88267.1"
                     /translation="MSGCATAPSIVREGNLSALRGQSLASLEGPSAAVDGTLAAAIQT
                     GVLKRLADNGADVSGVKPPAYLLQVGVGVSAPAVGVSGVAAPSFKETAWRSAPTKRHF
                     WNRRGPAQTATAVVLEVSTGKPAAWATVRADGADAETTADRLVRALAAPAP"
     gene            complement(2676..2852)
                     /locus_tag="PLH31_03180"
     CDS             complement(2676..2852)
                     /locus_tag="PLH31_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88268.1"
                     /translation="MKLLVWMHHMADRLGVVPGRRASKGWILRFAAAIMASLALWVLF
                     LWGLLLIVDGIAPL"
     gene            complement(2849..3196)
                     /locus_tag="PLH31_03185"
     CDS             complement(2849..3196)
                     /locus_tag="PLH31_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88269.1"
                     /translation="MNQASMSARIEDSRAANVAILNPLVQEITDALLDLGGAAHRDAV
                     VAHVAKKRGVFRPSAAMAQELDAAFSTYCSGASDRHAAGLLHLPYGPLSRRWALTEQA
                     YGLLRDGADAGSR"
     gene            complement(3398..>4572)
                     /locus_tag="PLH31_03190"
     CDS             complement(3398..>4572)
                     /locus_tag="PLH31_03190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR88270.1"
                     /translation="GAPLIEMASGDTRLTKAAGGLYWQVSTPRGSERSVSLWDQTLKA
                     PVRSEADAWSARIAPGPFDDRILIVERSIRPDRNGPAVLIQVASDEKILRLARQEFGR
                     DLALFLAGLWVVLSAAAAAQVSLGLAPMARVRDDLARLRRSPSARLSDDHPREIAPLA
                     QAINALAEAREADLARARRRAGDLAHSLKTPLSALSAQSRRAREAGAVEAADGLDSAI
                     AAVGAALEAELARARAAAARETSLAAETAPLAVVERLVEVIERTAEGERLMFDIEIDP
                     ALSIPVSDDVLTEMLGALIENAARFGRRRVRITAIATTGHVVLGVDDDGPGNDGRTEA
                     ALARGGRLDEAGPGHGLGLAIVRDLAEASGGMLRMDRSPLGGLRAEIAWLNPRAAS"
BASE COUNT          701 a         1625 c         1527 g          719 t
ORIGIN      
        1 cgaccacctg acggcggcag gcgcgcgcac catcgtctac gatgtgctgt tcagccaacc
       61 gggggccaat cccggtgacg atgtcgccct ggccagttct atcgccctgt cggggcgggt
      121 ctacctgccg cagttcatcg agcccggctt cggcgagggc gccccacagc aggtcgttgg
      181 tcctctgccg gagatcgccc ggggcgcggc gggtctgggc tcggcccatg tgctgttcga
      241 cgcggacggc gtggtcaggc gcatggagcc cttccaggcc ttcggccccg acctgacccc
      301 ggatctgatg accgtggtcc atcgcgccac ccgcgcgttc gacacccgcg acatcacgcg
      361 gggcgccgaa accaccctgt tgatcccctt cgccggcgcg cccaaccgct ttccgcgcgt
      421 atcgttttcg agtgtcctgg ccggtgatat cccgtcggag ttgctcgccg gtcgcacggt
      481 ctttgtgggc atgaccgcgc cgggccttgg tccggccttt cctaccccgg tgacgccgga
      541 cgccgccagc atgaccgggg ttcaactgca ggccagcatt ctggatggtc tgcgcagcgg
      601 aaccatgatt cggccagcgg ggctgatgag ccgcatcgcc ttcgccctca gcctgctctg
      661 gctgctgctg gcgggcctgt tggtattgcc gccacgcgcc aatctgatgc tggtcgtggg
      721 gttatcgagc ctggccctgg cgctcagcgc ggccttcttc tggagccttg atctgtggct
      781 ctcgcccgtg gcgacgatca ttggccttgc gaccgtgtcg ctggtctggg gctggggcca
      841 gctgggtcgg gtcagtgatc tgttgggcca cgaactgcgc cggctgctgc ccgtggccca
      901 gacgccgccg gccgagggct tcgagagcga tctggtggcc cgtcaggccc tgagcctggg
      961 cgctgcgatc aaccggatcg acgacctgcg ccgcttctcg gccgacgcgc tgttcagcct
     1021 gcccgacgcg accgtggtgc tggacgcccg gcgacgtgtg gtggccgcca acgccgccgc
     1081 ccagaccttg ttgcgcgcaa cccacttgcc gctggccggg gcttcggtgg cctccctgct
     1141 caacgccctc gagcccttgg gccgaacctt tgacgagtcc tggccacccg aggccgaacg
     1201 gatcgagccc gtcgacctgc atttgagcga cggccgcgcc tatcaatccc agaccgtgtt
     1261 tcgccgcgac gaggccggcg aaagcgcggg ctggatcgtc cgcctggccg acatcaccac
     1321 attgactgcc gccatgcgcc agcgcgagca ggccttgcaa ctgctcaccc acgacatgcg
     1381 gtcgccccag acctcgatcc tggcgctgtt ggcgacgacg ccggacctgc cagaagacgt
     1441 gtcggatcgt ctggcggcct gcgcccgtcg caccctggcc ctggccgacg gcttcgtgca
     1501 gctggcgcgc gccgaggtga cgccagtcgc gatggagccc gtagacctgt gcgacgtcgc
     1561 ggtcgaggcg gtggacgagc tgtggccgca gtgccgccag cgtcatatcg agatcgagca
     1621 ggagggctgc gacgcgccgc tgatggtgct gggcgatcgc agcgtactgg cgcgggccct
     1681 ggtcaacctg cttggcaatg cggtcaagta cagcccggaa gggtcgcgga tcagggtgtc
     1741 ggcgcgcgcc acccgcgagg acggccgcgc cttcgttgat gtctcgatca gcgatcacgg
     1801 tccggggctg tcgccggaag aggcggcgat ggcgttccgg gccttccagc ggttcgaccg
     1861 gcccgggtcg gaggccgctg gcggggtcgg tcttggcctg gcgttcgtcg acgcggccgc
     1921 tgcgcggcat ggcggcggcg ttctgtgcgc cagcgcgccc ggcgccggag caacctttac
     1981 gctgcgcctg ccggaatatc ggcccgccta aagcttctga cccagggccc tttcggccag
     2041 ccgtgacgaa gtcgtcaggt cggaaagggc tttagggagc gggcgcggcg agggccctga
     2101 ccaggcgatc ggctgtcgtc tcagcatcgg cgccatcggc ccgcaccgtg gcccaggcgg
     2161 ctggctttcc ggtcgagacc tccagcacca ccgccgtggc cgtttgggcc ggaccgcgac
     2221 ggttccagaa atgccgcttg gtcggcgcgc tgcgccaggc ggtttccttg aacgacggcg
     2281 cggcgacgcc ggacaccccg acagcaggcg ccgaaacgcc gacgccgacc tgaagcaggt
     2341 aggccggtgg cttgacgccc gagacgtcgg cgccgttgtc cgcaaggcgc tttagaacac
     2401 cggtctggat cgcggcggcc aaggtcccgt caaccgccgc cgatggcccc tccagcgacg
     2461 ccaggctctg gccccgcaga gccgacagat tgccctcgcg gactatgctc ggcgccgtgg
     2521 cgcaaccgct cacagctaga gctgccccaa ggctaaagac caagatgcgc attgaacccg
     2581 ctctccctgt ccccgccatc gcggtggggc tcgcccaaag cgaaatctag ccgccgcccc
     2641 gcgtgcggtc acccgctgat cacgcgagga ggcgtttaga gcggcgcgat gccgtcgaca
     2701 atcagcagca gcccccagag aaacagcacc cagagcgcca ggctggccat gatggcggcc
     2761 gcaaaccgca ggatccagcc cttgctggcg cgccgacctg gaaccacgcc caggcggtca
     2821 gccatatggt gcatccagac caacagcttc accgcgaccc ggcgtccgcg ccgtcacgca
     2881 gcagaccata ggcctgttcc gtcagggccc agcgacggct gagggggccg tagggcagat
     2941 gtagcaagcc tgccgcgtga cggtcgctgg cgccgctgca ataggtggaa aacgccgcgt
     3001 ccagctcttg ggccatcgcg gccgacggcc gaaagacccc acgcttcttg gccacatggg
     3061 ccacaaccgc atcgcgatgc gccgccccgc ccagatcaag cagcgcgtcg gtgatttcct
     3121 ggaccagcgg attgaggata gccacgttgg cagccctgct atcctcgatc cgcgcgctca
     3181 tgctcgcctg gttcatactc gcccctcaac tgcttcacac aacctttaaa gagcatggac
     3241 caaggctctg ccagaggtag agggggtgtt tttacaatgc gttacaaaga tcgcgtcaca
     3301 gctgaaaaga agaccgatct ccggtcagag acgatccctg cgcacctccc agacttcgca
     3361 aggtttcaga tgtgtagaac gagcattggg gcggccatca ggacgccgcg cgtgggttga
     3421 gccaagcgat ttccgcccgc agccctccga gcggcgagcg atccatccgc agcatacctc
     3481 ccgaggcctc ggccaggtcg cggacgatcg ccaggccaag gccgtgtcca gggccggcct
     3541 catcgagcct gccccctcgc gccagggcgg cttcggtccg gccgtcgttg ccggggccat
     3601 catcatcaac accgaggacc acgtgcccgg tcgtcgctat cgcggtgata cgcacgcggc
     3661 gacgcccaaa acgcgcggcg ttctcgatca gggcgcccag catctcggtc agtacgtcgt
     3721 ccgagaccgg aatgctcagg gctggatcga tctcaatgtc gaacatcagc cgctcgccct
     3781 cggcggtgcg ctcgatcacc tcgaccaggc gctcgaccac ggccaggggc gccgtttcgg
     3841 cggcgaggga cgtctcgcgc gcagcggcgg cgcgggcgcg ggccagctct gcttccagcg
     3901 ccgcaccgac ggcggcgatc gcgctatcga gaccatcggc cgcctcgacc gcgccagcct
     3961 cgcgcgcccg ccggctctgg gcggacagcg ccgagagcgg tgtcttgagg ctgtgggcca
     4021 ggtcgccggc ccgtcgacgc gcgcgggcca gatcggcctc gcgcgcctcg gccagggcgt
     4081 tgatcgcctg cgccaggggc gcgatctcac gcggatgatc gtcagaaaga cgcgccgacg
     4141 ggctgcggcg caggcgggcc agatcgtccc tgacccgcgc catcggcgcc aggccaagac
     4201 tgacctgcgc agccgcagca gctgatagaa cgacccacag gccggccagg aaaagcgcca
     4261 ggtcccggcc aaattcttgc cgcgccaaac gcaagatctt ctcgtcgctg gccacctgga
     4321 tcagcaccgc cggaccattc cggtcaggcc ggatgctgcg ttccacgatc agaatgcgat
     4381 cgtcaaaggg gcccggggcg atgcgggctg accaggcgtc ggcctccgaa cgcaccggcg
     4441 ccttcagggt ttggtcccac agcgacacag aacgttccga gccacgcggc gtcgagacct
     4501 gccaatagag acctccagca gccttcgtca gccgggtgtc gcctgacgcc atttcgatca
     4561 gcggcgcgcc ct
//
LOCUS       DAOMEO010000094         4568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2328_length_4568_cov_2.661201, whole genome shotgun sequence.
ACCESSION   DAOMEO010000094 DAOMEO010000000
VERSION     DAOMEO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_4568_cov_2.661201"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..2819
                     /locus_tag="PLH31_03195"
     CDS             6..2819
                     /locus_tag="PLH31_03195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQR88271.1"
                     /translation="MTASPPTLDLAPALVVLPGPRAGYADGGGEGRMLRAPDARDLME
                     HGPVLVAHAAMTAKRLNLHAPARSGRLFDVLELYAFTRPATFCAPSAVGLSMALGLAE
                     PKGAAEQAASLRISADALLAELRLTPQPSREEALAVAETLAKAGWAWGPAVIGALRSA
                     PVGNQFRSSGLDVWSRIIEWEDQAPLGESGTRPIDPERAGERLTELLQRAGLDEVREA
                     QATYAREAAFVFQPRDREGEPNMMLAEAGTGVGKTLGYLAPASLWAEANGPSVWISTY
                     TRALQRQIERESQSIYPDPAVRARKAVVRKGRENYVCLLNFQEQVNGAQLGNGDLIGL
                     ALTARWARATRDGDMTGGDFPAWLPTLFSIAPMQQASPANLVDRRGECVHAGCQHYRI
                     CYIEKAVRASKRADIVIANHALVLTQAAFDGARAARGLKGDNETTAVKRVVFDEGHHL
                     FDAADSAFSAALSGAEAAELRRWIRGPEGRGRRGRGLEARLLDILGDREGARQALTAV
                     LHAAAALPGEGWSGRVAPPDGQVNPIGPIESYLVAVIEQLRARSSERGGVEQGLQCAA
                     RPAVDLVRERAGEAAKALAGVEAPLLALARQLEDVLDEDAEHLGPSERARIEGALRGL
                     DRRARMTLPAWRSILKAIDEDAEDDPDFVDWFEATFLYGRVVDAACRRHWVDPTEPLR
                     AAVLSPAHGVLVTSATLTDPALEDPFALAEMRTGAARMPQSPKVLRLVSPFDYETNAR
                     AIVVTDVNKEDARQVSTAMRELFLAAGGGGLGLFTAIRRLKAVHEMIAAPLADRGLAL
                     YAQHVDPLEAGALVDIFRAEQDACLLGTDAIRDGIDVPGRSLRLLVFDRVPWPRPDIL
                     HKARRARFGGKGYDDSTARARISQAFGRLIRRADDRGMFVMLDAAAPTRLFSGLPEGI
                     TVERLCLVDAIEATAAFLTEASV"
     gene            2870..3319
                     /locus_tag="PLH31_03200"
     CDS             2870..3319
                     /locus_tag="PLH31_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88272.1"
                     /translation="MIRVLGIAAAMMLLAGGANAQEFVRTDCRTTVQSTHTLKFEDPK
                     HALWYKRFWTGSCADLSLCMPGSPNWNDIVSKLLIKGGPADRGVLLPKACRLGQMIGM
                     EWARDRRIKRIKTADLKTFNSILEASGDAVRGVEQVELKARSMISHR"
     gene            3397..3684
                     /locus_tag="PLH31_03205"
     CDS             3397..3684
                     /locus_tag="PLH31_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88273.1"
                     /translation="MIMSAHSMDPAIDEAAAPAQAYSARALVRAVRWRSGLSAQQFAR
                     VYHIDPDRLDALEHGDARPDAALTAYLRVIDHAPDVVREALERRALTGPAS"
     gene            complement(3681..4055)
                     /locus_tag="PLH31_03210"
     CDS             complement(3681..4055)
                     /locus_tag="PLH31_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR88274.1"
                     /translation="MAKVSYYTVGSNKLEEAKVFYDALLGSIGMTPMFDHPSGGRLYR
                     GKGTGLFGVLGPYDGNPACVGNGSMGGFSFDTVEEVAAFHAKALELGATDEGPPGERM
                     PGVYFAYFRDLDGNKLCGYKFG"
     assembly_gap    4248..4257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4258..>4568)
                     /locus_tag="PLH31_03215"
     CDS             complement(<4258..>4568)
                     /locus_tag="PLH31_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88275.1"
                     /translation="HSQPLAGADRPRHLTQIRLLVAEDNPANRKVVALLLQEAPVSLT
                     FAEDGAQALDAWRTQPFDLILMDVNMPVLTGLEAVREIRRAEPPDQRTPIWMLTANVF
                     D"
BASE COUNT          687 a         1537 c         1617 g          717 t
ORIGIN      
        1 ctcgcgtgac cgcttcgccg ccgactctgg atcttgctcc cgcgctggtc gtcctgcccg
       61 ggccccgcgc tggctatgcg gatggcggcg gcgaggggcg gatgctgcgc gcgccggacg
      121 cccgcgacct gatggagcat ggcccggttc tggtcgccca cgcggcgatg acggccaagc
      181 gactgaacct gcatgccccg gcccgcagcg gccgactgtt cgacgtgctg gagctctatg
      241 ccttcacccg gccggcgacg ttctgcgcgc cgtcggccgt gggcctgtcg atggccctgg
      301 gcttggccga gcccaagggc gcggccgagc aggcggccag tctgcggatc tcggccgacg
      361 ccctgctggc cgagctgcgc ctcacgcccc agccgtcgcg cgaggaggcc ctggcggtcg
      421 ccgagaccct ggccaaggcc ggctgggcct ggggccccgc cgtgatcggc gccctgcggt
      481 cggctccggt cggcaaccag ttccgaagct ccggcctgga tgtctggtcg cgcatcatcg
      541 agtgggaaga tcaggccccc ctgggcgaat ccggcacgcg gccgatcgac cccgagcgcg
      601 ccggcgagcg tctgaccgaa ctgctgcaac gcgcgggcct ggacgaggtg cgcgaggccc
      661 aggcgaccta tgcgcgcgag gccgccttcg tcttccagcc gcgcgaccgc gagggcgagc
      721 caaacatgat gctggccgag gccggcaccg gcgttggcaa gaccctgggc tatctggccc
      781 ccgcctcgct gtgggccgag gccaatgggc catcggtctg gatcagcacc tacacccgcg
      841 ccctgcagcg ccagatcgag cgggagagcc agtcgatcta tcccgacccg gcggtgcggg
      901 cccgcaaggc ggtcgtgcgc aaggggcgcg aaaactatgt ctgcctgctg aacttccagg
      961 aacaggtcaa cggcgcccag ctgggcaatg gcgacctgat cggcctggcc ctgacggcgc
     1021 gttgggcaag ggcgacccgc gacggcgaca tgaccggcgg ggatttcccg gcctggctgc
     1081 cgacgctgtt ttcgatcgcg ccgatgcagc aggccagccc ggccaacctg gtggaccggc
     1141 gcggcgagtg cgttcacgct ggctgccagc actatcgcat ctgctacatc gagaaggccg
     1201 tgcgggcctc caagcgggcg gacatcgtca tcgccaacca cgccctggtg ctgacccagg
     1261 cggcgttcga cggcgcgcgg gcggcgcggg ggctgaaggg tgacaacgag accacggccg
     1321 tcaagcgggt ggtgttcgac gaaggtcatc acctgttcga cgccgccgac agcgcctttt
     1381 cggcggccct ctctggcgct gaagcggcgg agctgcggcg ctggatccgg ggacccgagg
     1441 gacgcggtcg gcgcgggcgg gggcttgagg cccgtctact ggacatcttg ggcgaccgcg
     1501 aaggggcgcg tcaggccctg actgccgtgc tgcacgccgc cgccgccctg cccggcgagg
     1561 gctggtcagg ccgcgtcgcg ccgccggacg gacaggtcaa tccgatcggg ccgatcgaga
     1621 gctatctggt cgccgtcatc gagcaattgc gggcccgtag cagcgagcgc ggcggggtcg
     1681 agcaggggct gcagtgcgcc gcgcggccag cggtggacct ggtgcgcgaa cgggccggcg
     1741 aggcggccaa ggccctggcc ggggtcgagg cgccgctgct ggccctggcc cgccagctgg
     1801 aagacgtgct ggacgaagac gccgagcatc tgggcccctc tgaacgcgcc cgcatcgaag
     1861 gcgccctgcg cggcctggac cgccgcgcgc gcatgacgct gccggcctgg cgctcaattc
     1921 tcaaggcgat tgacgaggac gccgaggacg accccgattt cgtcgactgg ttcgaagcca
     1981 ccttcctcta tggccgcgtg gtcgacgccg cctgtcgccg gcactgggtg gacccgacag
     2041 agcctctgcg cgcggcggtg ctgagccccg cccacggggt gctggtcacc agcgccaccc
     2101 tgaccgatcc ggcgctggag gatcccttcg ccctggccga gatgcggacc ggggccgcgc
     2161 ggatgccgca gtcgcccaag gtgctgcggc tggtctcgcc gttcgactat gagacgaacg
     2221 cccgggccat cgtggtcacc gacgtcaaca aggaggacgc ccggcaggtt tcgacggcga
     2281 tgcgcgagct gttcctggcg gccggcggcg gcgggctggg gctgttcacc gccattcggc
     2341 ggctgaaggc cgtacacgag atgatcgctg cgcccctggc cgaccgggga ctggcgctct
     2401 acgcccagca tgtcgatccg ctggaggctg gggcgctggt cgacatcttc cgggccgagc
     2461 aggacgcctg cctgctgggc accgacgcga tccgcgacgg gatcgacgtg ccgggccggt
     2521 cgctgcgcct gctggtcttt gaccgggtgc catggcccag gcccgacatc ctgcacaagg
     2581 cccgccgcgc ccggtttggc ggcaagggct atgacgactc caccgcgcgc gcgcgcatca
     2641 gccaggcctt cggacgcctg atccggcgcg cggatgatcg gggcatgttc gtgatgctgg
     2701 acgccgccgc gcccacacgt ctgttctccg gcctgccgga gggcattacc gtcgagcggt
     2761 tgtgtctggt tgacgcgatc gaggccaccg ccgcattttt gacggaagcg tcggtttaat
     2821 ggttgctgtg tacagtgtgc cttccggggg gagggtgttg ggaggtcacg tgatcagggt
     2881 tttggggata gctgcggcga tgatgctgtt ggcgggcggc gccaatgcgc aggaatttgt
     2941 ccgtacagac tgccgaacga cggtgcagtc cacccacacg ctgaagttcg aagatcccaa
     3001 acatgcgctc tggtacaagc gcttctggac gggatcgtgc gctgacctca gcctgtgcat
     3061 gcccggctcg ccaaactgga atgacatcgt cagcaagctg ctgatcaagg gcggtcctgc
     3121 cgatcgcggt gtcctgctgc ccaaggcctg tcgtctgggg cagatgatcg gcatggaatg
     3181 ggcccgggac cggcggatca agcgcatcaa gaccgctgac ctgaagacct tcaattccat
     3241 ccttgaggcc tcgggcgacg ccgtgcgcgg cgtggagcag gttgaactga aggcccgctc
     3301 gatgattagc caccgctgat ctcaggtccg actcaaaggc gtcgaatttc tgcaatcgag
     3361 gcggactaga agggcctcct gtaaaggagc gtacccatga tcatgtccgc ccattcgatg
     3421 gaccccgcca tcgatgaagc cgcagccccg gctcaagcct attcggcccg cgcgctggta
     3481 cgggccgtac gctggcgcag cggcctgtcg gcccagcagt tcgctcgagt ctaccatatc
     3541 gatcccgacc ggctcgacgc gctggagcat ggcgacgccc gaccggacgc cgccctgacc
     3601 gcctatttac gagtgatcga ccatgcgccc gacgtggtgc gcgaggcgct ggagcgccgg
     3661 gctctgacag ggccagccag ctagccgaac ttgtagccac acagcttgtt gccatccaga
     3721 tcgcggaaat aggcgaaata gacgccgggc atgcgctcgc cgggcgggcc ctcgtcggtg
     3781 gcgcccagtt ccagggcctt ggcgtggaag gcggcgacct cttcgacggt gtcgaagctg
     3841 aacccgccca tagagccgtt gccgacgcag gcagggttgc cgtcataagg acccagcacg
     3901 ccaaacaggc ccgtgccctt gccccgatac agacggccgc ctgaagggtg gtcgaacatc
     3961 ggcgtcatgc cgatcgagcc cagcagggcg tcgtagaaga ccttggcctc ttcgagtttg
     4021 ttggaaccga ccgtgtagta agacaccttc gccatgcgct tctcccgttt gtgcttgggg
     4081 ataaggctag catgcggcgg ccagccggca acgctgacgc aaggtcaggc ttcgggcgcg
     4141 gtccctatca ggccttcggc gacctcggcc agcaggttga acaaggccgg cacgtcgatc
     4201 ggcttgcgca atactccatc agcgccgttc gccatgtaac gggcgatnnn nnnnnnncgt
     4261 cgaagacgtt ggcggtcagc atccagatcg gcgttctctg gtccgggggc tcggcccggc
     4321 gaatctcgcg caccgcctcc agcccggtca gaaccggcat gttgacgtcc atcaggatca
     4381 ggtcgaacgg ctgggtgcgc caggcgtcca gcgcctgtgc gccgtcctcg gcgaaggtca
     4441 gggacaccgg ggcctcctgt agcagcagcg ccactacctt gcggttggcg gggttgtcct
     4501 cggccaccag caggcggatt tgggtgaggt ggcgcggccg gtccgccccc gcgagcggct
     4561 ggctatgg
//
LOCUS       DAOMEO010000095         4559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2331_length_4559_cov_2.160746, whole genome shotgun sequence.
ACCESSION   DAOMEO010000095 DAOMEO010000000
VERSION     DAOMEO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_4559_cov_2.160746"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1119
                     /locus_tag="PLH31_03220"
     CDS             121..1119
                     /locus_tag="PLH31_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88276.1"
                     /translation="MCVQRFKVDIKETVEIDAAKVYRLYALTNDASADHWSAEFDGGL
                     PKAISGTADHQGGEILAAGVKSIASIQALGLGPSLPTVGVSGKDADALVAALKALRAK
                     PLTPEKLNDLKIALEAAKPQVLAPIDITLPVHKVYTVADFSEGVLIVHDALPVALRAD
                     CSPAPDLLSGDDPGDGIAVSASRACQLTILSRADYEAAKALDAVGRVAFKPAQASLAR
                     VKVWALDSSRVATLPLDRTTLIKTTTAYTFTAGRASKVDFDRPAPALQVVSLPFKLIG
                     AAVGGFVDTAKGETSKVQARTDLTKANTAELEAAAALAKVRKDQADAAKAAATPTP"
     gene            1165..1947
                     /locus_tag="PLH31_03225"
     CDS             1165..1947
                     /locus_tag="PLH31_03225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HQR88277.1"
                     /translation="MAALTAQGDMPQAPLAPALVMSPPEGLAEADVQILGGRKVADPE
                     ATWPATLKFNGGAGPCTATAIGPHVVLTAAHCVLPNQTGKITVGTVNPFSTTISCQHH
                     PDYDEDVKLDVALCHATAAIRLPGANSAYETVRISEPPRPDTPVVLLGYGCRQELGYG
                     PAGVLFEGDNKIFRITTGDPWIETRGGVAVCSGDSGGGAFVPTGTLRRWMFGVNSQGD
                     LLTRSMITSLADPKVAKFLKDWRRDGRDVKICGIDEMDDCHG"
     gene            1997..>3899
                     /locus_tag="PLH31_03230"
     CDS             1997..>3899
                     /locus_tag="PLH31_03230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HQR88278.1"
                     /translation="MSAALAQEVVDVRLTGERQTQTVLAIARVGQSEKVLVQKDQTFE
                     SIVQTKCGVVHPAYVQAFASLNGLTNGAAVANLTADATYEFPACAQAPLAESIPIAPK
                     QKSLKSILEKAGAVGIDVSKLDGFQPIGALPSLNTEVDYEAVLKSEGRSGQMALENFV
                     GRANAQMLAVLNPDLSPRRMTPGEALVIPGQAPTWTSISLKSGVSADEAKTQIEEAIK
                     ADKGKAEVTEVVTANLISGVGGYGVGYCPGPHPSWPYSIDTLLEARRANDSARPEGVY
                     PRPVKILILDTGFDGAILTQDSIPRDRIADVLGPVPPNPFRTKLGVNLADNQMTAMPP
                     PGLANRLHGSEVAVMALGGRHALRSRELGGIPIQVAFASVATQTPNLSARAIGKAIGF
                     AKRNDIAVINVSLMATAELAEFETYLKTGEDVLMVAAAGNDRQEFGGTTPTWPGGNGG
                     ALSHAQGGNVITVGSHDQRGAISPFSRFGGEYVDLLAPGCLMPTFTAGAEGGPAVDVE
                     REGTSFSAPLVSNIAAQLISEGVEVAFVKNRILASVDVDAELEDVVYSRGRLNLRKAL
                     SVWRDVVEYHEGAEDGPVEIKTGSVLRPGKILKPCTSDVEVGRLMKLSFTQRVGEPKK
                     AHVWLRPEPS"
     assembly_gap    3900..3909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4039..>4559)
                     /locus_tag="PLH31_03235"
     CDS             complement(4039..>4559)
                     /locus_tag="PLH31_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HQR88279.1"
                     /translation="AKRENSSSNRSSFSGAGHYAVPSGYVTDFASIPGFAKLLFPPFG
                     TWAEAAVIHDWLYDVGAKGRKAQADRIFKEAMTDMKVGGLRKTVMHWAVSLGGGGAYR
                     AAERRKPQDWAEHFVDRQGRARPAPFPQPVEAVWKRDFDCSRLEDQEEIWALQEDYLT
                     QFPNAAWGRAGG"
BASE COUNT          870 a         1444 c         1414 g          821 t
ORIGIN      
        1 ggcgtcgacg actactttgc ggctaagcgc gccatcgacg aggtcgttga gcgcgccaaa
       61 cgcaacgtcc aactgatggg cgtcaatcgt gacggcgtca ccgacgatgg tagggatcca
      121 gtgtgcgtcc agcgcttcaa ggtcgacatc aaagaaaccg tcgagatcga tgccgccaag
      181 gtctatcgcc tctacgcgct gaccaatgac gcctcagccg accactggag cgccgagttc
      241 gacggcggct tgcccaaggc gataagcgga acggccgacc atcaaggcgg cgagattttg
      301 gcggccggcg tcaagtcgat cgcctcaatt caggcgctcg gccttgggcc cagcctgccg
      361 accgtcggcg tctcgggtaa agacgccgac gccctggttg cggccttgaa ggcgttgcgt
      421 gccaagccgc tcacccctga gaagctcaac gatttaaaga tcgcattgga agccgccaag
      481 cctcaagtgc ttgcgccgat cgacatcacc ctgcctgtcc acaaggtcta cacggtcgcc
      541 gatttcagcg aaggcgtgtt gatcgttcac gacgccctac ctgtggcgtt acgggccgat
      601 tgctcgcctg ctccggactt gctttctggc gacgatccgg gcgacggtat cgcggtttcg
      661 gcctcacggg cttgccaact gaccatcctt tcgcgcgccg actatgaggc cgcaaaggcg
      721 ctcgacgcgg tgggccgggt cgctttcaag cctgctcagg cgtccttggc cagggtgaag
      781 gtctgggcgc tcgacagcag ccgggttgcg acgctgccac tggaccgcac gaccctgatc
      841 aagacgacca cggcgtacac cttcaccgcc gggcgggcta gcaaggttga tttcgatcgc
      901 ccggcgcccg ctctccaagt ggtctcgctg ccgttcaagc tcattggcgc ggccgtgggc
      961 gggtttgttg ataccgccaa gggcgagacc agcaaagtcc aggctcgcac cgatttgaca
     1021 aaggccaata cggcagagct cgaagccgcg gccgcgctcg ccaaagttcg caaagatcag
     1081 gccgacgccg ccaaggctgc ggccacgccc acaccttagg tataaagcga catgcgcgcc
     1141 ctaatcgcta tggcttgggc ttcagtggcc gccctgaccg cccagggcga catgccccaa
     1201 gcccccttgg cgccggccct ggttatgtct ccgcccgaag ggttggccga ggcggatgtc
     1261 caaattcttg gcggtcgcaa ggtcgccgat cctgaagcga cctggcccgc gactttgaaa
     1321 ttcaacggcg gagcaggtcc gtgcacggcg acggcgattg gtccgcatgt cgtgctcacc
     1381 gcagcccatt gcgtgcttcc caaccagacg gggaagatta ccgttggcac cgtcaacccg
     1441 ttcagcacca ccattagctg tcagcaccac ccagactatg atgaggacgt caaacttgac
     1501 gtcgcactgt gccatgcgac cgccgcgatc aggcttccgg gcgccaattc ggcttacgaa
     1561 accgtccgca taagtgaacc gccaagaccg gacacccctg tggtgttgct gggatatggc
     1621 tgtcgacagg agctgggcta cggtccggca ggcgtgctgt tcgaaggcga taacaagatt
     1681 ttccgcatca cgaccggtga cccctggatc gaaacccgcg gcggagtggc ggtctgttca
     1741 ggcgacagcg gtggcggagc ctttgtaccc actggcacgc tgcgtcgatg gatgttcggc
     1801 gtgaactccc agggcgacct actgacgcgc tcgatgatca cctcgctggc tgatccaaag
     1861 gtcgccaagt ttctgaaaga ttggcgccgc gacggtcggg acgtgaagat ttgcggcatc
     1921 gacgagatgg atgactgcca tggttgatcg ttgggcccgg ggcggattgt gcatggcatt
     1981 gacgatgtcg ggggccatgt cggcagccct ggctcaagag gtggttgacg tacggttgac
     2041 cggagagcgt caaacccaaa cggtattggc catcgccaga gtagggcaga gcgagaaggt
     2101 gctcgtccaa aaggaccaga ccttcgagtc gatcgtacag accaagtgcg gagtggttca
     2161 tcccgcctat gttcaggcct tcgccagcct caacggcttg accaacggcg cggcggtggc
     2221 gaacctgacg gccgacgcga cctatgagtt tcctgcgtgc gcccaagcgc ctctggccga
     2281 atccatcccg atcgctccaa agcaaaaatc actgaagtcc atcctagaga aggccggcgc
     2341 ggttggcatc gacgtgtcta agctcgatgg ctttcaacct atcggcgcct tgccgtcgct
     2401 gaacaccgag gtcgactatg aggccgtgct gaagtccgaa ggacgctccg gccagatggc
     2461 gttggaaaac tttgtcggtc gcgccaacgc gcaaatgctg gccgtgctca atcccgacct
     2521 ttcgccacgt aggatgacgc caggcgaagc cctggtgatc ccgggacagg cgccgacctg
     2581 gacctctatc tcgctcaagt cgggcgtatc ggccgacgaa gccaaaacgc agatcgagga
     2641 ggcgatcaaa gccgacaaag gcaaggccga ggtgaccgaa gtggtgacgg ccaacctaat
     2701 ctccggcgtc ggcggctacg gtgtgggata ctgccctgga ccccacccgt cctggcccta
     2761 ctcgatcgac accttgctcg aagcccgtcg ggccaatgac agcgctcggc cagaaggcgt
     2821 ttacccgaga ccggtcaaga tcctgatcct ggacaccggt tttgacggcg cgatcctgac
     2881 ccaggattcg atcccgagag atcggatcgc cgatgttctg gggcccgtcc ctcccaatcc
     2941 cttcagaacc aaactgggcg tcaacctagc cgacaaccag atgaccgcca tgccgccgcc
     3001 cggacttgcc aatcgcttgc atggctcgga agtggcggta atggccctgg gggggcgcca
     3061 tgcgctgaga agtcgagagc tgggcggcat accgattcag gtcgccttcg ccagtgtcgc
     3121 gacccagacg cctaacctta gcgcccgcgc gatcggcaag gctatcggct tcgccaagcg
     3181 caacgacatt gctgtgatca acgtctcgct gatggccacc gccgagctgg ccgagttcga
     3241 gacctatctg aagaccggcg aggacgtgtt gatggttgcc gccgccggta acgatcgtca
     3301 ggagtttggc gggacgaccc cgacctggcc ggggggcaat ggcggcgcgc tctcccacgc
     3361 tcaaggcggc aacgtgatta ccgtcggatc ccacgatcaa aggggcgcca tctcgccatt
     3421 ctctcggttt ggcggcgaat acgtcgatct tctcgcgccg ggctgcctga tgcccacttt
     3481 cacggctgga gccgagggcg ggcctgcggt cgatgtcgag cgcgaaggca cctccttctc
     3541 ggcgccgctc gtcagcaata tcgccgcaca actgatctcg gagggtgttg aggtcgcgtt
     3601 cgtcaagaac aggatactgg ccagcgtcga cgtagacgcc gagctcgagg atgtcgtcta
     3661 ttcccgtggg cgcctcaacc tgcgcaaggc gctgagcgtc tggcgcgatg tcgtcgaata
     3721 ccacgagggc gcggaggacg ggccagtgga gatcaaaacc ggttccgtat tgcgcccggg
     3781 taagatcctc aaaccctgca cctcggacgt cgaggtcggt cggctaatga aactcagctt
     3841 cacgcagcgt gtgggcgagc ccaagaaggc ccatgtttgg ctaaggcccg aaccctcccn
     3901 nnnnnnnnng cgcaaagcgc tctgcaccgc tcagaacctc gcgggccaga agatcacctt
     3961 gatcgaggac gggacgagaa gcaggatcga catcccgctg tcgcacctaa tcgacttcgt
     4021 accggccttt ctcgggccct agccgcccgc gcggccccag gcggcattcg ggaattgcgt
     4081 caggtaatcc tcctgtagag cccagatctc ttcctggtcc tcgagcctgg agcagtcgaa
     4141 atcgcgcttc cagaccgcct ctactggctg agggaagggc gctgggcgcg cgcgaccctg
     4201 ccggtcgacg aagtgttcgg cccagtcctg cggtttacgg cgttcggcgg ctcgataggc
     4261 tccaccgccg cccaggctga cagcccagtg catgacggtc ttgcgtagcc cgcccacttt
     4321 catatccgtc atcgcttcct tgaagatgcg atcggcctgg gccttccgac ccttggcccc
     4381 cacgtcgtag agccagtcat ggatgaccgc cgcctcggcc caggttccga acggcggaaa
     4441 gagcagcttg gcgaagccgg ggattgaagc gaaatcggtc acgtagccgg aaggcacggc
     4501 gtagtgacct gctcctgaaa aactggaccg gtttgagcta gaattttctc gttttgcgg
//
LOCUS       DAOMEO010000096         4540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2339_length_4540_cov_2.275808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000096 DAOMEO010000000
VERSION     DAOMEO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2339_length_4540_cov_2.275808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>273
                     /locus_tag="PLH31_03240"
     CDS             <1..>273
                     /locus_tag="PLH31_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HQR88280.1"
                     /translation="AILNQGVIEQVGTPAQVHDQPISPFVCGFVGEANRFEGGVLNGR
                     FTAGSVVLPAARVADGKAVAFVRPHDLVLDADGFAVQVNRVHVQGAL"
     assembly_gap    274..283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(376..1361)
                     /locus_tag="PLH31_03245"
                     /pseudo
     CDS             complement(376..1361)
                     /locus_tag="PLH31_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            complement(1433..2350)
                     /locus_tag="PLH31_03250"
     CDS             complement(1433..2350)
                     /locus_tag="PLH31_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQR88281.1"
                     /translation="MMLARVADSLYWLGRYIERAEHLSRLSSVMLNATLDQTDSGVQA
                     VWIALSAVGEPEVSSAIGSFEAAEALVLDRADDNSVVSSLSRARENARQVRDQITTET
                     WERLNLLYLKVTDENARRDFADGSTTFLHDVIADLHLFKGAADTTMSHGESWRFMMLG
                     MYLEVCFAETPGRQINDHIALVSLLRMACALEPYLRVYTAEIEPRHILEFLVFDEDFP
                     RSIRFATSQIEANLSVLARRAGGDGAGAGPERIAGRLKARLQFADINELENQGAGALL
                     TTVVNECARIHEAIYETFVAYPLEMRLPA"
     gene            complement(2347..3870)
                     /locus_tag="PLH31_03255"
     CDS             complement(2347..3870)
                     /locus_tag="PLH31_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQR88282.1"
                     /translation="MAAKIQDLEAPTLPMTEAAYLPGVAYDEMFAPDGAVRSHYDPLH
                     ARMSTLGGDELAERQQTLERSFLLQGITFTVYGGESTTERIIPTDLFPRIIPATEWAQ
                     IEAGLTQRLLALNLFLDDIYGDQQILMDGVVPRELVLGAPSYRREMQHIYVPHKSYAN
                     VCGSDLIRCQDGQFAVLEDNLRVPSGVSYMLANRDAAKRTFPGTYRAAGVRPVERYPD
                     LLLATLKSMSPEWRPNPQVVVLTPGVYNSAYYEHAYLARLMGVPLVEGRDLVVHDNMV
                     YMRTTTGIRRVDVIYRRVDDDFIDPLTFRRDSSLGAAGLFNAYRAGNVVICNAPGTGV
                     ADDKAVYAYVPEIIKYYLGQDAILPNIETFLCREPTQMGYVLANLDKLVVKAVGASGG
                     YGMLVGPHATQKERDEFAIVIKADPDNYIAQPTIQLSTAPCLVDGRIEPRHVDLRPFI
                     LSGEKTVVTPGALTRVALKRGSLVVNSSQGGGSKDTWVLADDNHAHSASPGNGGVRP"
     gene            4257..>4540
                     /locus_tag="PLH31_03260"
     CDS             4257..>4540
                     /locus_tag="PLH31_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HQR88283.1"
                     /translation="MLSTSRSAPQSRAEARRRARDAGDDVVAVLRAEGYYAYAVEPDI
                     SEGDPPRGVVKVTPGPVFVLANPNIVWRGAPPDEGVGQRATAAMRLVPGEP"
BASE COUNT          751 a         1430 c         1553 g          796 t
ORIGIN      
        1 gccattctca atcagggcgt catcgaacag gtcggcacgc ccgcccaggt tcatgatcag
       61 ccgatctcgc ccttcgtctg cggtttcgtg ggcgaggcca accggtttga gggcggggtc
      121 ctgaacggac gtttcaccgc cggctctgtg gttctgcctg ccgccagggt ggccgacggc
      181 aaggccgtgg ccttcgtgcg tccgcacgat ctggtgctcg acgccgacgg cttcgccgtg
      241 caggtcaatc gcgtccatgt gcagggcgcc ctgnnnnnnn nnnatcgagg tgacggtgtc
      301 gcgcgatcag gccgaccagt tcaccggggc ggtgaagctg tcggctcgca gggcgcatgt
      361 cttcccgggc tgagcctatt gctgctgctg ttgctgctgc tgatgaacgt cctgtcgcag
      421 ggcttcggcg ttctccgggc ggcgtcggcc gccggcgaag ttgggtcgcg ccatgcgaag
      481 gaactctggc tccgccaggg cagcacgggc ccggcgcacg gaaacgccca ccgccagctc
      541 gctgtctgag tcacccttgt agacgccgcg cgacggggtg acgtcgccat agtcgcgacc
      601 caccgcaaca gccacgtgcc gctcgccggt catcatgttg ttggtcggat caaacccgac
      661 ccagcgcagg ctgggcagga aaacctcgac ccaggcatgg gtggcgtccg ggtcagagcg
      721 gtcgcccgcg tcgcggtcgg tgaacaggta gcctgagacg tagcgcgccg gaatgttcca
      781 caccggcaga tcgccagcat gatgtgggca aagtcctggc agaccccgcg cccggcgctc
      841 agggccaggt cgatggggct gtcggcgtcg gtgacgccag gctggtactc gaacgcctga
      901 tagatggtct ccgaaagcac ccgcatcgcc gtcagcggat ccttgcggcg caggtcttcc
      961 agattgtgct cgttgacgaa ggcttccagc ccctcggtgg tctggacaaa gccgtgcatc
     1021 cgcagaaagt cgaagcactc gccgcgcacg aactcgctgc gcagccggtc ccattcaccc
     1081 atatcgaggg cctggggtcg ttcgctcgga gcctcggttt cgaccgccga gcgggcgacg
     1141 atggtcagct tgtcgtgcgg ctggggcacg tcgaaatgat agaccgcgtt gccgaagctg
     1201 tcggcgtagg agaacacctg cgccgcggga ttaaggtcca gctcgaagct gatcaggcgc
     1261 tggcgagccg tcttctgggg ctgcatccac agctccatca ggctttcccg aaccgggcgc
     1321 tcgtagtggt actgcgtgac gtggcgaatt tcgaggagca ctggggagtc cttactgggt
     1381 cgcgtgacgg gcgccgaaac cgctggcact ttcggctgtc acgctcgcgg tcctaagccg
     1441 gaagacgcat ttcgagcggg taggcgacga aggtctcgta gatcgcttcg tggatgcgcg
     1501 cgcattcatt gacgacagtg gtcagcaagg cgccggcccc ctggttttcc agctcattga
     1561 tgtcagcaaa ctgcagtctg gccttcaggc gtccggcgat ccgttcagga ccggcgccag
     1621 ccccgtcgcc gccggcgcgg cgcgccagta cgctgagatt ggcctcgatc tgcgaggtgg
     1681 cgaaacggat cgagcggggg aagtcctcgt cgaagaccag gaactccagg atgtgccggg
     1741 gctcgatctc ggcggtgtag acccgcagat agggctccag ggcgcaggcc atgcgcagca
     1801 gactgaccag ggcgatatgg tcgttgatct gccggccggg cgtctcggcg aaacacacct
     1861 ccagatacat gcccagcatc atgaaacgcc agctctcgcc gtggctcatg gtggtgtcgg
     1921 cagcgccctt gaacaggtgc aggtcggcga tcacgtcatg caggaaggtg gtcgagccgt
     1981 cggcgaagtc gcgcctggcg ttttcgtcgg tgaccttgag atacagaagg ttcagccgct
     2041 cccaggtttc ggtcgtgatc tggtcgcgca cctggcgggc gttttcgcgg gcgcgcgata
     2101 gcgacgagac gacggaattg tcgtcggcgc ggtcaagcac cagggcctct gcggcctcga
     2161 acgagccgat ggcgctgctc acttcgggct cgccgacagc ggagagggcg atccacaccg
     2221 cctgcacgcc cgaatcggtc tgatccaggg tggcgttcag catgacgctg gacaatcgcg
     2281 acaggtgttc ggcgcgttcg atgtatcgac cgagccagta gagactatcg gcaacccggg
     2341 cgagcatcat gggcgtacgc ctccattgcc gggacttgcg ctgtgggcgt gattgtcgtc
     2401 ggccaatacc caggtgtcct tcgaaccgcc gccctggctg gaattgacca ccagtgaacc
     2461 gcgtttcaga gccacgcgcg tgagcgcgcc gggcgtaacg acggttttct cgccggacag
     2521 gatgaagggg cgcagatcga cgtggcgcgg ctcgatccga ccatcaacga ggcatggcgc
     2581 ggtcgacagc tggatggtcg gctgggcgat gtagttatcg ggatcggcct tgatgacgat
     2641 ggcgaactcg tcgcgctcct tctgggtggc gtgcgggccg accagcatgc cgtagccgcc
     2701 cgaggcgccc acggccttga ccaccagctt gtccagattg gccagcacat agcccatctg
     2761 ggtcggctcc cggcatagga aggtctcgat attgggcagg atggcgtcct ggcccaggta
     2821 gtatttgatg atctccggca cataggcata gacggccttg tcgtcggcga cgccggtgcc
     2881 gggcgcattg cagatcacca cattgccggc gcgataggcg ttgaacaggc ccgcggcgcc
     2941 cagcgacgaa tcccgccgga aggtgagggg atcgatgaag tcgtcgtcga cccggcgata
     3001 gatcacgtcc acccggcgga tgccggtggt ggtgcgcatg tagaccatgt tgtcatggac
     3061 cacgagatcg cgaccctcga ccagtggcac gcccatcagc cgcgccagat aggcatgctc
     3121 ataataggcc gagttgtaga cgccgggcgt cagcaccacg acctgcggat tgggccgcca
     3181 ctcggggctc atgcttttca gcgtggcgag caacaggtcg ggatagcgct cgaccggccg
     3241 caccccggcg gcgcgatagg tgccggggaa ggtgcgcttg gccgcgtcgc gattggccag
     3301 catgtaggag acgcccgagg gcacccgcag attgtcctcc agcaccgcaa actgcccgtc
     3361 ctggcagcgg atcaggtcgc tgccgcagac attggcgtag gatttgtgcg gcacatagat
     3421 gtgctgcatc tcgcggcgat acgacggcgc ccccagcacc agttcgcgtg gcaccacccc
     3481 gtccatcagg atctgctggt cgccatagat gtcgtccagg aacaggttca gggccaggag
     3541 gcgctgggtc aggcccgctt cgatctgggc ccattcggtg gccgggatga tgcgcgggaa
     3601 aagatcggtc ggaatgatcc gctcggttgt gctctcgccg ccatagaccg tgaaggtgat
     3661 gccctgcagc aggaacgacc gttccagcgt ctgctgccgt tccgccaact catcgccgcc
     3721 cagcgtcgac atgcgggcgt gcagcggatc gtagtgcgag cgcaccgcgc catcgggcgc
     3781 gaacatctcg tcataggcga cgcccggcag atacgccgcc tcggtcatcg gcaaggtcgg
     3841 agcctcaaga tcttgaattt tcgccgccac gccttcgtcc acgcttgttc gccttgggtc
     3901 tgcgtcgagt cagacgcgcc atgagcaccc tagaacggcg cggcgtctga agtcggtgat
     3961 tttctttatc gccgcgccaa tgcgcacgac tgcctgtcat ttgggcgcct ttttcccgag
     4021 tagggaagcg ctcgccgtct tgcgtcccgc gcatttacgg ccgtgacagc ggcgtgaccg
     4081 gcgactacag ggggagcggg ggcgctccgc gcggggatta cggcttggtg cgtagacgat
     4141 tggccctcgg gatcgcgaca gcagcatggc tggctgctgg cggcgcgtgg gctgacgagc
     4201 cttcagcgac gatcaccggg gtagaagaca gggcgctgcg cgaggccatc cttcgcgtgc
     4261 tttccacatc caggagcgcg ccccagagcc gcgccgaagc gcgccgccgg gcccgggacg
     4321 cgggcgacga cgtggtcgcc gtgctgcgcg ccgagggcta ctacgcctat gccgttgaac
     4381 ccgacatcag cgaaggcgat ccgccgcgcg gcgtggtcaa ggtcacgcca ggaccggtct
     4441 tcgtgctggc caatcccaat atcgtctgga ggggcgcgcc gcccgatgag ggcgtgggcc
     4501 agagggccac agcggccatg cgcctcgtcc cgggcgagcc
//
LOCUS       DAOMEO010000097         4534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2342_length_4534_cov_2.467738, whole genome shotgun sequence.
ACCESSION   DAOMEO010000097 DAOMEO010000000
VERSION     DAOMEO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2342_length_4534_cov_2.467738"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /gene="lptD"
                     /locus_tag="PLH31_03265"
     CDS             <1..1829
                     /gene="lptD"
                     /locus_tag="PLH31_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HQR88284.1"
                     /translation="EKPTPTWSVAADKVVQDQNRQLVYYKNAVIRLWGAPLMYLPVFW
                     HPDPQATRSSGFLTPKLGASKRRGMSYQQPYLHVISPSQDIVLSPQINSSVNPFLNAH
                     WRKRFYSGSAEIRGGLTYEKDIDNRGERFGEATARSYILARGLFDINDKWKWGFSAER
                     TSDKLLFDNYTINDVYQQRGLFTSEDRRLSSQIYATRQDKRSYLSLSAVSVQGLRVSG
                     IDAVTGLASFENSDVFPLIGPLVEGRWQPESPVLGGRLRLQGAGVMLTRAESPYGATL
                     DGVDSQRGSLSADWRSTLTLKSGVRVSPFAQARGDAYRMSDLPGTEDSRTYSRALGVT
                     GVDFAYPFFKALKGGTIVLEPLAQVAAGSESRRVPIVVGTSGTTTYLYNEDSTSFEFD
                     ETNLFRANKSPGFDLYEGGQRLNVGARATVKYKDTRGASLLIGRSYRSEFDPLLPARS
                     GLQNKSSDWIVAATITPIRGVNASVRTRYAPDLASNKFRRIEAGVDAYHKIANGAVRY
                     IKDQQDAGGFPLETFELRGQLNLTKKWGITAYGQQDLVAGVWRRRDLGVVYADDCIRI
                     DVIYQNEDRFSQASTGGLKLQADESIVFRLTLATLGDTGYSQ"
     gene            1928..3076
                     /locus_tag="PLH31_03270"
     CDS             1928..3076
                     /locus_tag="PLH31_03270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR88285.1"
                     /translation="MKTLAAAGAASLIALAMASLGAPAFAQDAETAAPPVVAAPAAPP
                     RGLSESVAAVVNDDIVSSYDLMQRMRLLMISSGVQPTQENLPQLEQEALRSLIDEQVQ
                     LQELRRVEKAQKITIISTDKEVDDQIDEIAKGNSANMTGAQLLAQLAAQGVGAETFRS
                     QIRIDSSWQSWISGRYGSRLRVGEDQIKAYQRRLAESASKPQYQVSEVFIDAVRVGGM
                     ETAVNGATQLVAQMQQGAPFPAVARQFSSAATAANGGDAGWISPGEMPPEVDAALEEL
                     RPGQLSRPIPVRDGVYIIYLRDKRAGAKTALVDLKQVAYPLAADATAQQVEAANSALL
                     ALKPQITSCSTLEPAASKVDGLVAGDLGEAQITDLAPAFQAVPTPAFT"
     gene            3082..3222
                     /locus_tag="PLH31_03275"
     CDS             3082..3222
                     /locus_tag="PLH31_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88286.1"
                     /translation="MCGKRQSGGGAPTHDQIENRLRGQQLALISKRYLRDLRNSATIE
                     TR"
     gene            3219..4209
                     /gene="pdxA"
                     /locus_tag="PLH31_03280"
                     /pseudo
     CDS             3219..4209
                     /gene="pdxA"
                     /locus_tag="PLH31_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            4234..>4534
                     /locus_tag="PLH31_03285"
     CDS             4234..>4534
                     /locus_tag="PLH31_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HQR88287.1"
                     /translation="MSLADMPPLRDALERHDLLARKSFGQHFLLDLNITRKIARLAGI
                     GPADVVVEVGPGPGGLTRALLETGARVVAIEMDSRFMPILAELSEAADQRLEVVQG"
BASE COUNT          751 a         1638 c         1439 g          706 t
ORIGIN      
        1 ccgaaaagcc gacccccacc tggtcggtcg ccgccgacaa ggtggtccag gatcagaacc
       61 gccagctcgt ctactacaag aacgcggtca ttcgcctgtg gggcgcgccg ctgatgtatc
      121 tgccagtgtt ctggcacccc gatccgcagg ccacgcgcag ttcgggcttc ctgacgccca
      181 agctgggcgc gtccaagcgg cgcggcatgt cctaccagca gccctatctg catgtgatct
      241 cgccgtctca ggatatcgtc ctaagtcccc agatcaattc aagtgttaac ccgttcctca
      301 acgcccactg gcgcaagcgg ttctattccg gcagcgccga aatccggggc ggcctgacct
      361 acgaaaagga catcgacaac cgcggcgagc ggtttggcga ggccacggcg cgcagctaca
      421 tcctggccag gggcctgttc gatatcaacg acaagtggaa gtggggcttc agcgccgagc
      481 gcacgtccga caagctgctg ttcgacaact atacgatcaa cgacgtctac cagcagcgcg
      541 ggctgttcac gagcgaggac cgccgtctca gctcgcagat ctacgccacg cgccaggaca
      601 agcggtcgta cctgtccctt tccgccgtct ccgtacaggg cctgcgggtg tcggggatcg
      661 acgccgtcac gggcctggcc agtttcgaaa acagcgacgt ctttcccctg atcggaccgc
      721 tcgtcgaagg tcgctggcag ccggaatcgc cggtgctggg cggacgtctg cggctgcagg
      781 gcgccggggt gatgctaacc cgcgccgagt cgccctatgg cgcgacgctg gacggcgtcg
      841 acagccagcg cggcagcctg agcgccgact ggcgctcgac cctgaccctg aaatcgggcg
      901 tgcgcgtctc gccctttgcc caggccaggg gcgacgccta tcgcatgtcc gacctgcccg
      961 gaaccgagga cagccggacc tattcacgcg cccttggcgt gaccggcgtt gatttcgcct
     1021 accccttctt caaggcgctc aagggcggca cgattgtgct ggagcccctg gcccaggtcg
     1081 ccgccggctc cgaaagccgc cgcgtgccga tcgtggtggg aacctcgggg acgacgacct
     1141 atctctacaa cgaagacagc acctcgttcg agttcgacga gaccaacctc ttccgcgcca
     1201 acaagtcgcc gggctttgat ctgtatgagg gcggtcagcg cctgaacgtc ggcgcgcgcg
     1261 ccaccgtcaa gtacaaggat acccgaggcg ccagcctcct gatcggccgc agctaccgct
     1321 ccgagttcga ccccttgctg cccgctcgct cgggcctgca gaacaagtcg tccgactgga
     1381 tcgtggcggc caccatcacg ccgatccgcg gcgtcaacgc ctccgttcgc acccgctatg
     1441 cgccagacct ggccagcaac aagttccgcc gcatcgaggc gggcgtcgac gcctatcaca
     1501 agatcgccaa cggcgcggtt cgctacatca aggaccagca ggacgccggc ggcttcccgc
     1561 tggagacctt cgaacttcgc ggccagctga acctgaccaa gaagtggggc atcaccgcct
     1621 atggccagca ggacctggtg gctggcgtct ggcgccgccg cgatctgggc gtggtctatg
     1681 ccgacgactg catccgcatc gacgtcatct atcaaaacga ggaccgtttc agccaggcct
     1741 caacgggcgg cctgaagctg caagccgacg agtcgatcgt gtttcgccta accctcgcca
     1801 cattgggtga tacagggtac agccagtaaa ttagtctcgc gtcgccgccg gcgcgcgtga
     1861 ttttcgcgcg cgtgatcggc acgtcgcgcc tagaggaacg acactaccca tgcggcctgc
     1921 gcgtagcgtg aagacccttg ccgcggctgg cgccgcgtcc ctcatcgccc tggccatggc
     1981 gagcctcggc gcgcccgcct tcgctcaaga cgccgaaact gcagcgcccc cggtggtcgc
     2041 ggcgccggct gcgccgccgc gcgggctgtc ggagagcgtt gcggctgttg tgaacgacga
     2101 catcgtctcc agctacgacc tgatgcagcg catgcgcctg ctgatgatca gctctggcgt
     2161 ccagcccacc caggaaaacc tgccccagct ggagcaggaa gccctgcgct cgctgatcga
     2221 tgagcaggtc cagctgcagg aactgcgacg ggtcgagaag gcccagaaga tcacgatcat
     2281 ctcgaccgac aaggaagtcg acgaccagat cgacgagatc gccaagggca acagcgccaa
     2341 catgaccggc gctcaactgc tggcccagct ggcggctcag ggcgttggcg ccgagacctt
     2401 ccgctcgcag atccgtatcg acagcagctg gcagagctgg atcagcggac gctatggctc
     2461 gcgcctgcgg gtcggcgaag accagatcaa ggcctatcag cgccgcctgg ccgagtcggc
     2521 cagcaagccg cagtatcagg tcagcgaagt gttcatcgac gccgttcgcg tcggcggcat
     2581 ggagacggcc gtcaatggcg cgacccagct ggtcgcccag atgcagcagg gcgcgccctt
     2641 cccggccgtc gcccgccagt tttcatcggc ggccacggcg gccaacggcg gcgacgccgg
     2701 ctggatcagc cctggagaga tgccccccga ggtcgacgcc gcgctcgaag agctgcgtcc
     2761 cggccagctg tcgcgcccga tcccggtccg cgatggcgtc tacatcatct acctgcgtga
     2821 caagcgcgct ggcgccaaga cggctctggt cgacctgaaa caggtcgcct atccgctggc
     2881 cgcggacgcg acagcccagc aggtcgaggc cgccaacagc gccctgctgg cgctgaagcc
     2941 tcagatcacc agctgctcca cccttgagcc cgccgccagc aaggtggacg gtctggtcgc
     3001 gggcgacctc ggtgaagccc agatcaccga cctggctccg gccttccagg cggtaccgac
     3061 gccggccttc acctgatcgc cgtgtgcggc aagcgtcagt cgggcggcgg cgcgccgacg
     3121 cacgaccaga tcgagaaccg cctgcgcggc cagcaactgg ccctgatctc caagcgctac
     3181 ctgcgcgacc tccgcaactc ggcgaccata gaaacccgtt gaagaccaag cccctggccc
     3241 tgagcgctgg cgatcccgcc ggcgtcgggg ccgagatcat cgccaaggcc tgggcggcgc
     3301 tccgttggga ggggccgccg ttcatggtgg tgggcgacgc ccggctgctg gcctcggccg
     3361 gctcgggcgt caaggtcaag accgtgacgg ggcctgcaga gaccctggac gttttcccga
     3421 ccgccctgcc cgttctggac gttgccctgc tgagccccgt tgtcgccggc cagccctcca
     3481 gcgcccacgc cgcccagatc atccgctgca tcgagaccgg cgccggtctg gcgctgtccg
     3541 gcgcggtgtc gggtctggtc accgccccca tcgccaaggc cccgctctac gatgcgggct
     3601 tcagctttcc gggccatacc gagtttctcg ccgaactgac cgccggagcg cctcacgagg
     3661 gtgttcgcgg cccggtgatg atgctgaccg ccggcggcct gcgcgccacc ctggtgacga
     3721 tccacaaggc gctgaacgat gtctcggccg ccctgtcggt ggaagccatc gtcacggccg
     3781 gcatggtgac caaccaggct ctcaaacgcg acttcggcat cgccagacct cgcctggcgg
     3841 tggcggccct caatccccac gcgggcgaga gcggggcgct gggccgcgat catcgagccc
     3901 gccgcccgcg tgctgcgcga cctgggcgtc gaggtcctgg gccccgcccc cgccgacacc
     3961 ctgttccacg ccgaagcgcg cgcccgctat gacgccgtgc tgtgcatgta tcacgaccag
     4021 gccctgatcc cggtcaagat gctcgacttc tggagcggcg tgaacgtcac cctgggcctg
     4081 cccatcgtcc gcacctcgcc cgaccacggc accggcttcg acattgctgg acgcggcctg
     4141 gcccgagccg atagcctgat cgccgccatc cggctggcgg cccagatcgc cgcccgccgc
     4201 gcggcctgac cttttcgttc gagatccaag accatgagcc tggccgacat gccgccgctg
     4261 cgcgacgccc tggagcgtca cgaccttctg gcccgcaaga gctttggtca gcacttcctg
     4321 ctggacctca acatcacccg caagatcgcg cggctcgccg ggatcggccc cgctgatgtg
     4381 gtggtcgagg tgggccccgg tcccggcggc ctgacccgcg ccctgctgga gaccggcgcc
     4441 cgcgtcgtgg ccatcgagat ggacagccgc ttcatgccga tcctggcaga gctctccgaa
     4501 gccgccgacc agcggctgga agtcgtacag ggcg
//
LOCUS       DAOMEO010000098         4524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2346_length_4524_cov_2.750503, whole genome shotgun sequence.
ACCESSION   DAOMEO010000098 DAOMEO010000000
VERSION     DAOMEO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_4524_cov_2.750503"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..2476)
                     /locus_tag="PLH31_03290"
     CDS             complement(368..2476)
                     /locus_tag="PLH31_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88288.1"
                     /translation="MLIIDTAPDLEQRRLAALWALDILDSLEEDGFNDLVAEVSETCD
                     TPISLISLIDTDRQWFKARVGLDAQETPRDVAFCNHAIQTPALLVIEDVSLDPRFRDN
                     AFVTGAPGIMAYAGAPLTTVDGLRIGALCAIDTRPKRWTPRELRCLERLSRVAMTMIE
                     TRAGARVAVAQIQTLEDLRRSDQRYRTIIAAMREGVVLQDQASVIIEHNAHAARLLGM
                     TSDQLAGRSSVDPDWSLVTAEGAPLTVDQQPSMQCLRTGKPVLDFMLGVNLPNRDQRW
                     LNVNSLPIFEGDGPTPAQTLTTFVDITQLKRQETALIEAVAAAERASAAKSTFLANVS
                     HEIRTPLNGVIALAKALSRLDMPDRQREMVGIIVNSGESLERILNDLLDIAKIEASRM
                     ELEIAPFDLAAEIQTCTDLMRLRADGKGIAFEVAFSSEAKGFFLGDALRLRQVVANLT
                     ANAIKFTDSGGVSLTVEVEPLAADDCRLTITVNDTGIGLDADVLPLLFERFVQGDDTF
                     TRRFGGTGLGLAICKNLVTLMGGKIEARALPERGSSFAFSVPLRRAEAPAAAASESSA
                     PAVRTDRRLRVLLAEDHPVNRRVVELLLEPLEVELVMAENGAQAFATFSKSQFDVVLM
                     DMQMPEMDGLSATASIRRWEAAQQRSPTPVVMMTANASTDHREKAMGAGADVFITKPV
                     TPDSLYAGLNAALSLSTAAA"
     gene            complement(2588..>3571)
                     /locus_tag="PLH31_03295"
     CDS             complement(2588..>3571)
                     /locus_tag="PLH31_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR88289.1"
                     /translation="EARGIVRQEGKIASVQQNGETGFVTSVTLESGQVVEGDLFIDCS
                     GFRGLLIEGTLQTGYEDWSQWLLNDRAVAVPCALGGSMAPVTRATARPAGWQWRIPLQ
                     HRLGNGYAYSSAHISEDEATAYLLAHLDGEPLAAPNHIRFTAGRRKKSWNRNVVAIGL
                     AAGFMEPLESQSIHLIQVGIARLLAMFPDREFRQPDIDRYNRVMAFEYEKIRDFIILH
                     FKATQRSDTPYWDYLREMSVPDYLADKMAVFESYGRIFRENEELFNDTSWFAVMVGQG
                     IVPKGYDPMVDVMSDDELRRRMAEIRSVIAKSAEVMPGHMAFIRANCDAMA"
     assembly_gap    3572..3581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3582..4096)
                     /locus_tag="PLH31_03300"
     CDS             complement(<3582..4096)
                     /locus_tag="PLH31_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR88290.1"
                     /translation="MTRVNRLRKVVIVGGGTAGWMTAAALGRFLTKDGQTSVTLVESD
                     SIGTVGVGESTIPQINIFNRMLGVDENDFIRKTRATFKLGIDFVDWKQIGHSYHHPFG
                     PYGVDMEAVSFHAYWLKAHAMGEDDDLSAYSLNALAARQHKFMRPNGQPNSPLGSIAY
                     AFQFDAGLYAK"
     gene            complement(4093..>4524)
                     /locus_tag="PLH31_03305"
     CDS             complement(4093..>4524)
                     /locus_tag="PLH31_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR88291.1"
                     /translation="DKGFDPIEIAEYNRLTILQTELIRDFIILHFKANERPEPYWQRA
                     REMAIPESLQRKIDLFAARGRLFQSDYDLFAEPSWIAVMLGQNIRPRNYDPLVDTLDE
                     TLVRGQMQRLAALVRQTADALPSHEAFIARYCAAPEFEKTR"
BASE COUNT          764 a         1399 c         1556 g          795 t
ORIGIN      
        1 gccgatgaac gccgacgaca ggcaggtagc gccaatcagg gccatgcggc ggccagattt
       61 atggcgtgcg gatgaagaca tgtttctctc cccttgagac ggcgctgtgc gcgctcggtt
      121 gtccttgccc gccgccttcc gatggtagcg gtctcagagt gagagggtta cgaggtaagg
      181 cctttcaagt caacgtggtc ataccttgtg ggttgtgtgt ggtccaattg gcgtgaaatc
      241 gtgtcgcaaa gccggcctgg gaccgccggc gcaggaccgc tggcggcctg aagcgttgaa
      301 tcgggccggg gccgttaagg gcgaaggtta taggtcagac cttgttgcgc ctgccggcgc
      361 cgtgggctca ggctgcggcg gtgctgagcg acaaggcggc gttgagcccg gcatacaggc
      421 tgtcaggcgt caccggcttg gttatgaaca catcagcgcc ggcgcccatc gccttttcgc
      481 gatggtcggt cgaggcgttg gccgtcatca tgacgaccgg tgtcggcgat ctttgctgcg
      541 ccgcttccca gcgccggatg ctggcggtgg ccgacaggcc gtccatttcc ggcatctgca
      601 tgtccatcag gacgacgtcg aactgcgact tgctgaacgt cgcaaaggcc tgagccccgt
      661 tctcggccat caccagctcg acctccagcg gttcgagcag caattcgacg acccggcgat
      721 tgaccggatg atcctcggcc agcaggacgc gaaggcgccg atccgtgcgc accgccgggg
      781 cgctggattc cgacgcggcc gcagcgggcg cctcggcgcg gcgtaagggc acgctgaagg
      841 caaagctgct gccgcgttcg ggaagcgccc gcgcctcgat cttgccgccc atcagggtga
      901 ccaggttctt gcagatggcc aggcccagtc cggtgccgcc aaagcgccgc gtgaaagtgt
      961 cgtcgccctg cacaaagcgc tcgaacagca gcggaaggac atccgcatcc aagccgatcc
     1021 ccgtgtcgtt gacggtgatg gtcagccgac agtcgtccgc tgcgagaggc tcgacctcga
     1081 ccgtcaggct gacgccgccg ctgtcggtga acttgatggc gttggccgtc aggttggcga
     1141 ccacctgtcg caaccgcagg gcgtcgccca ggaagaagcc cttggcctca gacgaaaagg
     1201 cgacctcaaa ggcgatcccc ttgccgtcgg cgcgcaggcg catcaggtcg gtgcaggttt
     1261 ggatctcggc cgcaagatcg aagggcgcga tctccagttc catgcgcgag gcctcgatct
     1321 tggcgatgtc gagcaggtcg ttgagaatgc gctccagcga ctcgccagag ttaacgatga
     1381 tgcccaccat ctcgcgctgt cggtcgggca tgtcgaggcg cgacagggcc ttggccaggg
     1441 cgatgacgcc attgagcggc gtgcggatct cgtggctgac attggccagg aaggtcgatt
     1501 tggccgcgct ggcgcgctcg gcggcggcga ccgcctcgat cagggcggtc tcctgacgct
     1561 tcagctgggt gatgtcgacg aaggtggtca gggtctgggc gggggtgggg ccgtcacctt
     1621 cgaagatcgg cagggaattg acgttcagcc agcgctggtc gcggttgggc agattgacgc
     1681 ccagcatgaa atccagaacc ggcttgcccg tgcgcaggca ctgcatcgaa ggctgctggt
     1741 cgacggtcag gggcgcgccc tcggccgtga ccaggctcca gtcgggatca acagaggaac
     1801 ggccggccag ctggtcgctg gtcatgccca gcaggcgcgc ggcatgggcg ttgtgctcga
     1861 taatgacgct ggcctggtcc tgcagcacca cgccttcgcg catggcggcg atgatggtgc
     1921 gatagcgctg gtcgctgcgg cgcaagtcct cgagcgtctg gatctgggcg accgcgaccc
     1981 gggcgccggc ccgcgtctcg atcatggtca tggcgacgcg cgacaggcgt tccaggcagc
     2041 gcaactcgcg gggcgtccag cgcttggggc gagtgtcgat ggcgcatagg gcgccgatcc
     2101 gcaagccatc gaccgtggtc aggggcgcgc cggcataggc catgatgccc ggcgctccgg
     2161 taacaaaggc gttgtcccgg aaccgggggt ccaggctcac atcctcgatc accaatagcg
     2221 ccggcgtctg gatggcgtgg ttacaaaagg ccacgtcgcg cggagtttcc tgcgcgtcga
     2281 gtccgacccg cgccttgaac cactggcggt cagtgtcgat caaagagatc agggagatcg
     2341 gcgtgtcgca ggtctcggag acctcggcga ccaggtcgtt gaagccgtct tcctcgagac
     2401 tgtcgagaat gtccaatgcc cacagcgccg cgagacgccg ttgctcaagg tcaggcgcag
     2461 tatcgataat caacatgccg cgccgccgcc ctctccctca tcgagataag ggagcatgca
     2521 cgcgcggata gggcgtttca ataaagggcg gcagggcgaa acgccacagg gcgcgggctg
     2581 gccggcctca ggccatcgca tcacaattgg cgcggatgaa cgccatgtgc cccggcatca
     2641 cctccgccga cttggcgatc accgaccgga tctccgccat ccggcgccgc agttcatcat
     2701 cgctcatcac gtcgaccatc gggtcatagc ccttgggaac aatgccctgg ccgaccatca
     2761 cggcgaacca cgaggtgtcg ttgaacagtt cctcgttctc gcggaagatc cggccatagc
     2821 tctcgaacac cgccatcttg tcggccaggt agtcagggac gctcatctcc cgcagatagt
     2881 cccagtaggg cgtgtcgctg cgctgggtgg ccttgaagtg caggatgatg aagtcgcgga
     2941 tcttctcgta ttcgaaggcc atcacccggt tgtagcggtc gatatcgggc tggcggaact
     3001 cgcggtcggg gaacatggcc agcaggcgag ctatgcccac ctggatcagg tggatcgact
     3061 ggctttccag cggctccatg aagcccgccg ccaggccgat ggcgacgacg ttgcggttcc
     3121 agcttttctt gcgccgaccg gcggtgaacc ggatgtggtt gggcgcagcc aggggctcgc
     3181 cgtccaggtg ggccagcaga taggcggtgg cctcatcttc gctgatatgg gcgctggaat
     3241 aggcatagcc gttgcccagg cggtgctgca gcgggatgcg ccattgccag ccggccggcc
     3301 gggccgtggc gcgggtgacc ggggccatcg agccgcccag ggcgcagggt acggccacgg
     3361 cgcggtcatt gagcagccac tgtgaccagt cctcatagcc ggtctgcaag gtcccctcga
     3421 tcagcaggcc ccggaaaccc gagcagtcga tgaacaggtc gccctcgacc acctggccgc
     3481 tctccagcgt caccgaggtg acaaacccgg tctcgccgtt ctgctgcacc gaggcgatct
     3541 tgccctcctg gcgcacgatg ccgcgcgcct cnnnnnnnnn naacttcgca tagaggccgg
     3601 cgtcgaactg gaaggcgtag gcgatcgagc ccaggggcga attgggctga ccgttggggc
     3661 gcatgaactt gtgctggcgc gccgccaggg cgttcagcga ataggcgctc agatcgtcgt
     3721 cctcgcccat ggcgtgggcc ttcagccagt aggcgtggaa cgacacggcc tccatgtcga
     3781 cgccataggg gccgaagggg tggtgatagc tgtggccgat ctgcttccag tcgacgaagt
     3841 cgatgcccag cttgaaggtg gcccgggtct tgcggatgaa gtcgttctcg tcgacgccca
     3901 gcatgcggtt gaagatgttg atctgcggga tggtcgactc gcccaccccc accgtgccga
     3961 tggaatcgct ctcgaccagg gtgacgctgg tctggccgtc cttggtcagg aagcggccca
     4021 gggcggcggc ggtcatccag ccggcggtgc cgccgccgac gatgacgacc ttgcggagac
     4081 ggttgacgcg ggtcatcggg ttttctcgaa ctcgggcgcc gcacaatagc gggcgataaa
     4141 ggcttcgtga gagggcaggg cgtcggcggt ctggcgcacc agcgcggcca ggcgctgcat
     4201 ctggccccgc accagggtct cgtccagggt gtcaaccagc gggtcatagt ttcgcgggcg
     4261 gatgttctga cccagcatca cggcgatcca cgagggctcg gcgaacagat catagtctga
     4321 ctggaacaga cggccacgcg cggcgaacag gtcgatcttg cgctgcaggc tttccgggat
     4381 cgccatctcg cgcgcccttt gccagtaggg ctcggggcgc tcattggcct tgaagtgcag
     4441 gatgatgaag tcgcggatca gctcggtctg caggatggtc aggcgattgt attcggcgat
     4501 ctcgatgggg tcgaaaccct tgtc
//
LOCUS       DAOMEO010000099         4524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2348_length_4524_cov_2.431864, whole genome shotgun sequence.
ACCESSION   DAOMEO010000099 DAOMEO010000000
VERSION     DAOMEO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_4524_cov_2.431864"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLH31_03310"
     CDS             <1..264
                     /locus_tag="PLH31_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HQR88292.1"
                     /translation="INTLIQFVIVAAVVFLLVKVVNGIRRADAAEPGPETPAAPTGEE
                     ALLMEIRDLLAKGAAPKVAVSAPKAAVAKAPAKAAVKPKPKAK"
     gene            complement(320..3166)
                     /locus_tag="PLH31_03315"
     CDS             complement(320..3166)
                     /locus_tag="PLH31_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR88293.1"
                     /translation="MIRPVAKAALVALALSTTALSPMAFAAQSTASAPSISVPPIVYK
                     ERVLANGLKVFTARDTTTPNVTVQVWYGVGSKDDPEGRSGFAHLFEHLMFKATRNMPN
                     ETLDRLTEDVGGFNNASTYDDFTNYYEVVPANHLERLLWAEADRLGSLVIDEAVFASE
                     RDVVKEELRQRVLADPYGRLFALYVPQQSFTSHPYKRPGIGSIEELDAATVDDVRAFH
                     ATYYRPDNAALIVAGNFDEAQLQALVDKYFAPLKTPAAPLPKVTVVEPPRTGPKTVNT
                     YGPNVPLPAVAITWLAPASADKDTPALAVLDAILSAGKSSRLYDTLVYDQQIAQTVFS
                     SGPTNAQPGLFYVGAVMAGGKTAQQGEAALLAQVARLRDAPPTAAELAEAKTGLLADA
                     VRRREEIDGRAFAIGYALLTDGDAARANTNLADLQAVTAADVQRVAKKWLADDRRTSI
                     RYLPESARPAGETDNTPVPPKVASVKFDGAITTLAPEAERQKIPAVAAPIAAVLPSPV
                     EKTLANGLRVIVAKSSDLPLITADLTVRGGASADPAGLAGASSLTADLLTEGTKTRGA
                     VDIARQTEALGANLAAGSGWEASSLTLSVIADKAAPAMAIMADVAQNPVFKAEELDRV
                     RAETLDGLSVAYQRPGSLAAYATAPVLYAGSAYGHVAGGTPGSLPKIKRDDLVKLHSA
                     AWRPDNAVLVLTGNLTPEAGFALAQTAFGGWKKPAAAPPALPAAPTGYKPRNVVIDLP
                     GTGQAAVTLAKPTITRADPNYYSGLVTNAVLGVGYSSRLNQEIRIKRGLSYGAGSSLT
                     PQGQFGGFSARVQTKNESAGQVVGLVQAELTRLAAEPASASELTARKSVLVGGFGRDL
                     GTSEGLADILGNLAVYGVPLNEIQSYAAKVEAVSASDVQAFAKSMMDPAQTSIIVAGD
                     LKAMGDTLPAAVPNATVIPAARLDLDSPTLTSGK"
     gene            complement(3255..3704)
                     /locus_tag="PLH31_03320"
     CDS             complement(3255..3704)
                     /locus_tag="PLH31_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HQR88294.1"
                     /translation="MPGRRMTGVPAEAPDGLWLFDGVCNLCSGSVAIVLKLDREGVIR
                     FTPIQSPYGRQLAAAHGIDPDQPASFLFLDGGRALQKTAAIGALLRRLKPPWRWLAVV
                     DRLPRGPADAAYDWLAANRYRLMGKKDACMVPSPADRARFILDPPDR"
     assembly_gap    3709..3718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3719..4247)
                     /locus_tag="PLH31_03325"
     CDS             complement(<3719..4247)
                     /locus_tag="PLH31_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQR88295.1"
                     /translation="MRRFLALVLTFALATPVLAEAEWTEKLRAGEIPIEAHADARGRG
                     GMVKAAIDIPAPAAVVWRTILDCDRAARMTPGVKHCRVITRAPDSRSETREHRVKWGF
                     FLPTLRSVSRVEFDPGRTIRFTCVSGDIRACEGSWTLAPIKGGTATRVTYEMWAAAPF
                     AVPVSVLSGLMRRDVP"
     gene            complement(4317..>4524)
                     /locus_tag="PLH31_03330"
     CDS             complement(4317..>4524)
                     /locus_tag="PLH31_03330"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666870.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HQR88296.1"
                     /translation="LWVRHEIGPFAAPDVIQWAPGLPKTRSGKIMRRILRKIAENELG
                     SLGDTSTLADPSVVDDLVKNRSGA"
BASE COUNT          687 a         1463 c         1662 g          702 t
ORIGIN      
        1 atcaacaccc tgatccagtt cgtgatcgtg gcggccgtgg tcttcctgct ggtcaaggtg
       61 gtcaatggca tccgccgcgc cgacgccgcc gagcctgggc ccgagacgcc cgcggccccg
      121 acgggcgagg aggctctgct gatggagatc cgggacctgc tggccaaggg agcggcgccc
      181 aaggtcgccg tatcggcgcc caaggcggct gtggcgaagg cccctgccaa ggctgcggtg
      241 aaacccaagc cgaaggccaa gtaagatcgt catcccggat cggccttgcg gccgtccggg
      301 atgacgaggc ttgaagcgcc tacttcccgc tggtcagggt cgggctgtcc aggtccagcc
      361 tggcggccgg gatcaccgtg gcgttcggca cggcggcggg cagggtgtcg cccatcgcct
      421 tcagatcgcc ggcgacgatg atgctggtct gggcggggtc catcatgctc ttggcgaagg
      481 cctgaacgtc gctggcgctg accgcctcga ccttggccgc atagctctgg atctcgttca
      541 gcggcacgcc atagacggcc aggttgccca ggatgtcggc caggccctcg gaggtgccca
      601 ggtcacggcc aaagccgccg accagcaccg acttgcgggc cgtcagttcg ctggccgagg
      661 ccggctctgc ggccagccgg gtcagctcgg cctggaccag gccgaccacc tgaccggcgg
      721 actcgttctt ggtctgcacc cgggccgaga agccgccgaa ctggccctgc ggcgtcaggc
      781 tggagccggc gccgtaggac aggccgcgct tgatgcggat ttcctggttc aggcgcgagg
      841 aatagcccac ccccagtacg gcgttggtga ccaggcccga atagtagttg ggatcggcgc
      901 gagtaatggt gggcttggcc agggtcacgg ccgcctggcc cgtgcccggc aggtcgatga
      961 ccacattgcg cggcttgtaa ccggttggcg cagcgggcag tgccggaggc gcggccgcag
     1021 gcttcttcca gccgccgaac gcagtctgcg ccagggcgaa gccggcttcc ggcgtcaggt
     1081 tgccagtcag gaccagcacg gcgttgtccg gacgccaggc ggcggaatgc agtttgacca
     1141 gatcatcgcg cttgatcttc ggcagcgaac ccggcgtgcc gccggccacg tggccgtagg
     1201 ccgaaccggc atacagcacg ggggcggtgg cgtaggcggc caggctgccc gggcgctgat
     1261 aggccaccga caggccgtcc agggtctcgg cgcgaacccg gtccagttcc tcggccttga
     1321 agaccgggtt ctgggcgacg tcggccatga tcgccatggc cggggcggcc ttgtcggcga
     1381 tgacgctgag ggtcagggag gaagcttccc agcccgagcc ggcggccaga ttggcgccaa
     1441 gcgcttcggt ctgacgggcg atgtcgaccg cgccgcgggt cttggtaccc tcggtcagca
     1501 ggtcggcggt caggctggag gcgccggcca ggccagccgg atcggcgctg gccccgccgc
     1561 gcacggtcag gtcggcggtg atcagcggca ggtcgctcga cttggcgaca atgacccgaa
     1621 gaccattcgc caaagtcttc tccactggcg agggcaggac ggcggcgatc ggggcggcca
     1681 cggccgggat cttctggcgc tcggcctcgg gggccagggt ggtgatcgcg ccgtcgaact
     1741 tcaccgaggc caccttgggc gggaccgggg tattgtccgt ctcgccagcc gggcgggccg
     1801 attccggcag atagcggatg gacgtgcgac ggtcgtcggc cagccacttt ttcgcaacgc
     1861 gctggacgtc ggcggcggtc accgcctgca aatcggccag attggtgttg gcgcgggcgg
     1921 cgtcgccgtc ggtcagcagg gcgtagccga tggcgaaggc gcggccatcg atctcctcgc
     1981 gacgacggac agcgtcggcc agcaggccgg tcttggcctc agccagttcg gcggcggtgg
     2041 gcggggcgtc gcgcagccgc gcgacctggg ccagcagggc ggcctcaccc tgctgcgccg
     2101 tcttgccgcc ggccatgacg gcgccgacat agaacaggcc cggctgggcg ttggtcggac
     2161 ccgacgagaa caccgtctgg gcgatctgct ggtcatagac cagggtgtcg tacaggcgcg
     2221 aagacttgcc ggccgagagg atggcgtcca gcacggccag ggcgggcgtg tccttgtcgg
     2281 ccgaggccgg ggccagccag gtgatcgcca ccgccggcag gggcacattg ggcccatagg
     2341 tgttgacggt cttggggccg gtgcggggcg gctcgaccac cgtcaccttg ggcaggggtg
     2401 cagccggggt cttcagcggc gcgaaatact tgtcgaccag ggcctgcagc tgggcctcgt
     2461 cgaaattgcc ggccacgatc agggcggcgt tgtcggggcg gtagtaggtg gcgtggaagg
     2521 cgcgcacgtc atcgaccgtg gccgcgtcca gttcctcgat cgaaccgatg ccggggcgct
     2581 tgtaggggtg gctcgtgaac gactgctgcg gcacatagag ggcgaacagg cggccatagg
     2641 ggtcggccag cacgcgctgg cgcagctctt ccttcacgac gtcgcgctcg gaggcgaaca
     2701 ccgcctcgtc gatcaccagc gagcccaggc ggtcggcctc ggcccacagc aggcgctcca
     2761 gatggttggc gggcaccacc tcgtagtagt tggtgaagtc gtcataggtg gaggcgttgt
     2821 tgaagccgcc cacgtcctcg gtcagccggt cgagggtctc gttgggcatg ttgcgcgtgg
     2881 ccttgaacat caggtgttca aagaggtggg caaagcccga gcggccctct gggtcgtcct
     2941 ttgagccgac gccataccag acctgcaccg tcacgttggg cgtggtcgtg tcgcgagccg
     3001 tgaacacctt caggccgttg gccagcacgc gctccttgta gacgatcggc ggcacgctga
     3061 tcgacggcgc gctggccgtg ctctgggccg cgaaggccat gggcgagagg gcggtggtcg
     3121 agagagccag cgccacaagg gcggccttgg cgacaggacg gatcattcgg agagctccag
     3181 caaacgcggt tgcccgaccc tagcatcgcc tcgcgagagc gaaaccttac cgtcctgtca
     3241 tgaagcggtg gcgttcatct gtcgggcggg tcgaggatga accgcgcgcg gtcggcgggg
     3301 gaaggaacca tgcaggcgtc cttcttgccc atcagccggt agcggttggc ggcgagccag
     3361 tcataggccg cgtctgcggg accccggggc aggcggtcga caacggccag ccagcgccag
     3421 ggcggcttca gtcgcctcag cagggcgccg atggcggcgg tcttctgtaa cgcacgcccg
     3481 ccgtccagga acaggaagct ggcgggctgg tcggggtcta tcccgtgggc ggcggccagc
     3541 tggcggccat acggcgactg gatcggcgtg aagcggatga cgccttcgcg gtccaacttc
     3601 agaacgatgg cgaccgatcc cgagcagaga ttgcagacgc cgtcgaacag ccacaggccg
     3661 tcgggcgcct cagccgggac gcccgtcatc cggcgtccag gcattcgcnn nnnnnnnncc
     3721 ggaacatcac ggcgcatcag gccgctcagg acggagaccg gcacggcgaa gggcgcggcg
     3781 gcccacatct cgtaggtcac gcgggtggcg gttccgccct tgatcggcgc aagcgtccag
     3841 gagccctcgc aggcgcggat atcaccgctc acgcaggtaa agcggatcgt gcggccgggg
     3901 tcgaactcca cgcgcgagac cgagcgcagg gtcggcagga aaaagcccca cttgacccgg
     3961 tgctcgcgcg tctcggaccg gctatccggc gcgcgggtga tcaccctgca atgcttgacg
     4021 ccgggggtca tgcgggctgc ccggtcgcag tccaggatcg tgcgccagac cacggccgcc
     4081 ggggcgggaa tgtcgatcgc cgccttgacc atgccgccgc ggcctctggc gtcggcatgg
     4141 gcctcgatgg ggatctcgcc ggcccgcagc ttttctgtcc attcggcctc cgccagaacc
     4201 ggcgtcgcca gggcgaatgt caggacgagg gcgaggaacc ggcgcatcat gtttacgcta
     4261 acgcgccggc gcaggttcgg ttaggtccaa acgccttttt cttgagaggc ctctgttcac
     4321 gcgcctgacc tgtttttcac cagatcgtcg acgacggagg gatccgccag ggtcgaggtg
     4381 tcccccaggc tccccagttc gttctcggca atcttgcgca ggatgcggcg catgatcttg
     4441 cccgagcggg tcttgggcag gcccggggcc cactggatga catctggcgc cgcgaagggg
     4501 ccgatctcgt ggcggaccca cagg
//
LOCUS       DAOMEO010000100         4514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2356_length_4514_cov_2.440009, whole genome shotgun sequence.
ACCESSION   DAOMEO010000100 DAOMEO010000000
VERSION     DAOMEO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_4514_cov_2.440009"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLH31_03335"
     CDS             <1..760
                     /locus_tag="PLH31_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR88297.1"
                     /translation="IAAKTAEAHPKLPGTIYSAAAEIEAAGGKALPLIVDVREESSVY
                     EAVEKAVAQFGGIDICVNNASAISITGTLSTEMKRYDLMHQVNGRGTFLTSKACIPHL
                     KKAENPHVLMLSPPLDMKPRWFAPHVAYSMAKYNMSLCVLGMAQEFASDGIAFNALWP
                     RTGIATAAIQFALTGEEGLRHCRTPQIMADAAHAIFEKPSRGFSGNFLIDDSFLYEQG
                     VTDFTPYAVDPTATLMPDFFVPEDSVPPPGVKIG"
     gene            936..1415
                     /locus_tag="PLH31_03340"
     CDS             936..1415
                     /locus_tag="PLH31_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR88298.1"
                     /translation="MPVTIRPAVAADSALILQFIRDLAEYEKLLDSVEATEGHVTAAL
                     FGENPRAFCDIAEIDGEPVGFALWFYNFSTFVGRHGIYLEDLFVRPEARGSGAGKALL
                     ANLARRCVDENLGRLEWSVLDWNAPSIAFYDSLGAAALDDWIIRRMTGDALRKLAGV"
     gene            complement(1412..>2312)
                     /locus_tag="PLH31_03345"
     CDS             complement(1412..>2312)
                     /locus_tag="PLH31_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88299.1"
                     /translation="LWPVFAVSVLFGASRAFLSPASSALGPMLAPRPLLPRAIAWNSL
                     AWQSASIIGPAVGGLLLIHSPALAFGASLALYLIAAVMALMIRQNTKPETQPGSRLEL
                     IKEGLSYVWNNKIVFGSISLDLFAVILGGATALLPVFAKDVLHVGPEGFGLLRAAPAV
                     GASLVAIYLASNPIRSKAGPIMFAGAAVFGAATVASGLSQTMWLSVGALALLGGADML
                     SVYVRQTLVQIVTPDAMRGRVAAVSGVFISASNELGEAESGVAAWFLGPVGAAVFGGV
                     GALGVTGLWAWLFPELRKADRLD"
     assembly_gap    2313..2323
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2324..2664)
                     /locus_tag="PLH31_03350"
     CDS             complement(<2324..2664)
                     /locus_tag="PLH31_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88300.1"
                     /translation="MSDLPPTAVEPPSDTSTRALLRERDFMLFWAERFISTLGVQIQS
                     VAVGWQVYAIARETRSVGESAFLVSMIGLAQFLPLLALTLIAGETADRRTRKLIVAVS
                     LAVDAVSASVLL"
     gene            2874..3398
                     /locus_tag="PLH31_03355"
     CDS             2874..3398
                     /locus_tag="PLH31_03355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQR88301.1"
                     /translation="MTSISPVLHSTVSAPALVLETPAMETAVTALIDRVFGPGRFAKV
                     SERLREGNHLLGDCSFVAMRGGQPVGCVRLWPVTIGGAPVAFLGPLAVDPDERSAGLG
                     QTLVEAACEAARAAGWKAVMLVGDAPYFARAGFSASQTAGVVMPGPVDQRRVLVKALV
                     PGGDEGLSGLVSVA"
     gene            3447..4022
                     /locus_tag="PLH31_03360"
     CDS             3447..4022
                     /locus_tag="PLH31_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HQR88302.1"
                     /translation="MTADPPKAGLEGVAAAVKVAGERGLPPVHLWHPAHCGEIDIRIR
                     KDGVWFHEGTPIGREALVRLFSTVLRLDPDGYHLVTPVEKMKITVEDAPFIAVRVDQV
                     GESLRFTTNVGDVVDAGPDNIIRVETDAVTGEPRPYLHVRRGLEALIARPVFYELAEM
                     ATSRQTPDGEVFGIVSNGAWFPIAPAGTVLA"
     gene            4019..>4514
                     /locus_tag="PLH31_03365"
     CDS             4019..>4514
                     /locus_tag="PLH31_03365"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQR88303.1"
                     /translation="MTPQERRDWITGRLDPIAHYNPSLADPVRSDFDLNPGLTFDNPH
                     ALRPAAVLVGLIEREDGLSVLLTRRSDTMRSHTGQIAFPGGRCDPGETPWQTALREAQ
                     EEVGLDPKFVTLSGLLHGYQTVTGFHVTPVVGFIDPAAEFVASPEEVADVFETPFDFL
                     MNPAN"
BASE COUNT          734 a         1531 c         1532 g          706 t
ORIGIN      
        1 catcgccgcc aagacggcgg aggctcatcc caagctgccg ggcaccatct attcggccgc
       61 cgccgagatc gaggcggccg gtggcaaggc cctgccgctg atcgtcgacg tgcgcgagga
      121 aagctcggtc tacgaagccg tagagaaggc cgtggcccaa ttcggcggca tcgacatctg
      181 cgtcaacaac gcctcggcca tctcgatcac cggcaccctg tcgaccgaga tgaagcgcta
      241 tgacctgatg catcaggtca acgggcgggg cacgttcctg acctccaagg cctgcatccc
      301 gcatctgaag aaggcggaaa atccgcacgt cctgatgctg tcgccgccgc tggacatgaa
      361 gccccgttgg tttgcgccgc acgtcgccta ttcgatggcc aagtacaata tgagcctgtg
      421 cgtgctgggc atggcccagg agttcgcatc ggacggcatc gccttcaacg ccctttggcc
      481 ccgcacgggc atcgccaccg ccgccatcca gtttgctttg accggcgaag aaggcctgcg
      541 ccactgccgc acgccgcaga tcatggccga tgcggcccac gcgatcttcg aaaagccctc
      601 acgggggttt tcgggcaatt tcctgatcga cgacagcttc ctgtacgagc agggcgtcac
      661 cgacttcacg ccctatgccg tcgacccgac cgccaccctg atgccggact tctttgtgcc
      721 ggaagacagc gtcccgccgc cgggggtgaa gatcgggtga ggccttgcgt ccttcgaggc
      781 tcacctgcgg ttcgcacctc aggatgaggg ctgttttctg aattcctcat cctgaggcgc
      841 ccgcgcagcg ggcctcgaag gacgcacgat gcctggcctt gccgagcggg atcaactggt
      901 cgagtaagac gtccagacag tccgaagagc ccctcatgcc cgtcaccatc cgtcccgccg
      961 tcgccgccga cagcgccctg atcctgcagt tcatccgcga cctggccgag tatgaaaagc
     1021 tgctggactc ggtggaggcg acggaaggcc acgtgacagc ggcgctgttc ggggaaaatc
     1081 ctcgagcgtt ctgcgacatc gccgagatcg acggcgagcc ggtgggcttt gccctgtggt
     1141 tctacaattt ctcgaccttc gtgggccggc acgggatcta tctggaagac ctgttcgtgc
     1201 ggccagaggc gcggggcagc ggcgcgggca aggccctgct ggccaatctg gcgcggcggt
     1261 gcgtggacga gaacctcggc cggctggaat ggtcggtgct ggactggaac gcgccgtcca
     1321 tcgccttcta tgacagcctg ggcgccgcgg ctctggacga ctggatcatc cggcggatga
     1381 ccggggacgc cctgcgcaag ctggcggggg tttagtccag ccggtcggcc ttcctcagtt
     1441 ccgggaacag ccaggcccac agtccggtga cccccagcgc gccgacgccg ccgaacaccg
     1501 ccgcgccgac cgggcccagg aaccaggccg cgaccccgct ctcggcctcg cccagctcgt
     1561 tggaggcgct gataaacacg cccgagacgg cggcgacgcg gccgcgcatg gcgtcggggg
     1621 tgacgatctg caccagggtc tggcggacat agacgctgag catgtcggcc ccgcccagca
     1681 gggccagggc gccgacagaa agccacatcg tctgcgagag gccagaggcc acggtggccg
     1741 cgccgaacac cgccgcgccc gcgaacatga tcgggccagc cttgctgcgg atcgggttgc
     1801 tggccagata gatcgccacc aggctggcgc ccacggcggg cgcggcgcgc agcaggccaa
     1861 agccttcggg tcccacatgc agcacgtcct tggcgaacac cggcagcagg gccgtggccc
     1921 cgcccaggat caccgcgaac aggtccagcg agatcgagcc gaagacgatc ttgttgttcc
     1981 agacgtacga aagcccctcc ttgatcagct ccaggcgcga gcccggctgg gtctcgggct
     2041 tggtgttctg gcggatcatc agggccatga cggcggcaat gagatagagg gccagcgacg
     2101 cgccgaaggc cagggcgggc gaatggatca gcagcaggcc gcccacggcc ggtccgataa
     2161 tcgaggcgct ttgccaggcc agtgagttcc aggcgatggc gcgcggcagc agggggcgcg
     2221 gcgccagcat cgggccaagc gcgctgctgg ccggcgaaag gaaggcgcgg ctggccccaa
     2281 acagcaccga aaccgcgaag actggccaca ggnnnnnnnn nnnagcagca cgctggcgct
     2341 gacggcgtcg acggccaggc tgacggcgac gatgagcttg cgggtccggc ggtcggccgt
     2401 ttcgccggcg atcagggtca gggccagcag gggaaggaat tgggccagac cgatcatgct
     2461 gaccaggaag gccgactcgc ccaccgaccg ggtttcgcgg gcgatggcgt agacctgcca
     2521 gcccacggcc accgactgga tctgcacccc cagggtcgag ataaagcgct cggcccagaa
     2581 cagcatgaag tcacgctccc gcaacagggc gcgggtagag gtgtcggagg gcggttcgac
     2641 ggccgtcggg gggaggtctg acatcgcgtc gcaccatagt ggcgctcgcg gcggtcgcaa
     2701 cgttattgac cttgcggagg cttggcccta aagcgcccgg caattgttga gaggtctggc
     2761 catgagcctg cccaagcggc tgcgacgaat gacgcgcgaa cgcgccacga tctgaagccc
     2821 tcccgcgcct tcgcgtcggg agctcccgac gacctttcgc atttcgaagg ctgatgacct
     2881 ccatttcccc cgttttgcat tccacggttt ccgcgccggc gctcgtcctc gagacgcccg
     2941 cgatggagac cgccgtcacc gccctgatcg accgggtgtt cggccccggg cgcttcgcca
     3001 aggtttccga gcgcttgcgc gagggcaacc acctgctggg cgactgctcg ttcgtggcga
     3061 tgcggggcgg ccagcccgtg ggctgcgtgc gcctgtggcc ggtgaccatc ggcggcgcgc
     3121 cggtggcgtt cctcgggccc ctggccgtcg accccgacga gcgcagcgcg ggcctgggcc
     3181 agaccctggt cgaggccgcc tgcgaggccg cccgggccgc gggctggaag gccgtgatgc
     3241 tggtcggcga cgcgccctat ttcgcccgcg ccggcttttc ggcgagccag accgccggcg
     3301 tcgtgatgcc cggacctgtc gatcagcgcc gcgtgctggt gaaggcgctg gttcctggcg
     3361 gcgacgaggg cctttccggg ctcgtctcgg tcgcctgaga cgttccgtcg cttgagccgg
     3421 acgatggcat ggcctacctg atagccatga ccgccgatcc tccgaaagct ggactagaag
     3481 gcgtcgctgc ggccgtgaag gtcgctggcg agcgcggcct gccgccggtg catctgtggc
     3541 atcccgccca ttgcggcgag atcgatatcc gcatccgcaa ggacggggtg tggttccatg
     3601 agggcacgcc gatcggccgc gaggcccttg tgcggctgtt ctcgaccgtg ctgcggctgg
     3661 atcccgacgg ctaccatctg gtcacccccg tcgagaagat gaagatcacc gtcgaggacg
     3721 cgccgttcat cgccgtgcgg gtcgaccagg tgggcgagag cctgcgcttt acgaccaatg
     3781 tcggcgacgt ggtcgatgcc gggcccgata atatcatccg tgtcgagacc gacgccgtca
     3841 ccggcgagcc gcggccctat ctgcatgtcc ggcgcggcct tgaggccctg atcgcccggc
     3901 cggtcttcta cgagctggcc gagatggcga cctcgcgcca gacgccggac ggcgaggttt
     3961 tcgggatcgt ctccaacggc gcctggtttc ccatcgcgcc agccggaacc gtcctggcat
     4021 gacgccccag gaacgccgcg actggatcac cggccggctg gaccccattg cgcactataa
     4081 tccgagcctg gccgacccag tgcgctcgga cttcgacctg aatccgggcc tgaccttcga
     4141 caacccccac gccttgcggc cggccgccgt gctggtgggg ctgatcgagc gggaggacgg
     4201 gctgagcgtg ctgctgaccc gacgctcgga caccatgcgc agccacaccg gccagatcgc
     4261 cttccccggc gggcgctgcg acccgggcga aacgccctgg cagaccgccc tgcgcgaggc
     4321 ccaggaagag gtggggctgg atccgaaatt cgtcaccctg tcgggcctcc tgcacggcta
     4381 tcagacggtg acgggctttc atgtcacgcc ggtggttggc ttcatcgatc cggcggcgga
     4441 atttgtcgcc agccccgaag aggtggccga cgtcttcgag acgccgttcg acttcctgat
     4501 gaacccggcc aatc
//
LOCUS       DAOMEO010000101         4505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2364_length_4505_cov_2.382472, whole genome shotgun sequence.
ACCESSION   DAOMEO010000101 DAOMEO010000000
VERSION     DAOMEO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_4505_cov_2.382472"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..282)
                     /locus_tag="PLH31_03370"
     CDS             complement(85..282)
                     /locus_tag="PLH31_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88304.1"
                     /translation="MQVSANAAIPYAPPRPASSGQAAAPIVDLTQLAVAQTNSKMTAA
                     AGAALGQVSGELTGVLVNISA"
     gene            complement(412..1437)
                     /gene="bioB"
                     /locus_tag="PLH31_03375"
     CDS             complement(412..1437)
                     /gene="bioB"
                     /locus_tag="PLH31_03375"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQR88305.1"
                     /translation="MTQTHAPQANNAPRHDWTLPQVEALFELPFMELVFQAASVHRAG
                     FDPSEVQLSQLLSVKTGGCAENCGYCSQSAHFKTGLKAEKLMDADEVIARARAARDGG
                     AQRFCMGAAWRELKDRDLPRLAAMIGGVKALGLETCATLGMLTADQAKQLKDAGLDYY
                     NHNLDTGPEYYGDVVSTRTYQERLDTLAYVRDAGMSTCCGGIVGMGETRRDRASLLHQ
                     LATLPSHPDSLPINGLVPVSGTPLGDKILRDGAKAIDSIEFVRTIAVARIVCPRSMVR
                     LSAGRDDMSRELQALCFLAGANSIFVGGKLLTTPLPGQDEDSALFQALDLRPMGVGRP
                     AEAVAAE"
     gene            1562..>2791
                     /locus_tag="PLH31_03380"
     CDS             1562..>2791
                     /locus_tag="PLH31_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQR88306.1"
                     /translation="MDREIELKFLIAPEAADQILSLLDGESDVRQLDATYFDTVDHAL
                     RKAGFGLRVRDGEGGRKQTLKSASAGGVFSRGEWETPIAGPGPDQKALAATPAAAVLN
                     GQALQPVFTTQVERIVRLVRRGETVIEAVVDRGALIAGSRRAVVCELELELKSGSPSA
                     LFELARDVARQVPLRLSLVSKAERGYGLANAAAGPPGRRSAVRLDPAMTVEQALHAAG
                     REALTHLCASADTLRDRPGPEGVHQLRVAVRRTRSLLKTFKPLAVDAAARSLALELKW
                     LAGELDAARNLDVFVADVWRPASEAEPAQDLAAFGRALLGAQAQAYRRVEAALESPRQ
                     RALLLETAAWLEAGAWTTEPALDAVRHRPAAGFAGEILDHMRQVVIERGESLDEMEPE
                     ARHRLRLKGKTLRYAAED"
     assembly_gap    2792..2801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2802..3013
                     /locus_tag="PLH31_03385"
     CDS             <2802..3013
                     /locus_tag="PLH31_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HQR88307.1"
                     /translation="AAAKALQDALGDLNDRAARARLAREVATAHGEPEAAFAAGRLTA
                     CGDDAEALGRARKAYATLAAAKVFW"
     gene            3126..>4505
                     /gene="ahcY"
                     /locus_tag="PLH31_03390"
     CDS             3126..>4505
                     /gene="ahcY"
                     /locus_tag="PLH31_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQR88308.1"
                     /translation="MADYIVKDISLADFGRKEIAIAETEMPGLMATRAEYGPAQVLKG
                     ARIAGSLHMTIQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAIAASGVPVFAFKGE
                     NLVEYWEYAHKIFEWHDGGYPNLILDDGGDATLLCVLGPKAEKDPTVLNNPQNEEEEA
                     LFAVMKKYLAEKPGFYTAIRDAIGGVSEETTTGVHRLYQMAAKGELPFPAINVNDSVT
                     KSKFDNLYGCRESLVDAIRRGTDVMLSGKVAVVCGYGDVGKGSAASLRQGGARVIVTE
                     IDPICALQAAMEGYEVQTLDDCAGRADIFVTATGNKDVITVDHMRAMRNNAIVCNIGH
                     FDSEIQVSGLKNFKWDEIKPQVHHIEFPDGKKIILLSEGRLVNLGNATGHPSFVMSAS
                     FTNQTLAQIELWTNKSAYKNDVYTLPKHLDEKVALLHLEKLGAKLSKLRPDQAEYINV
                     PENGPFKPDH"
BASE COUNT          756 a         1497 c         1542 g          700 t
ORIGIN      
        1 cgccgcgctc gaggcgctga gcgaactggt ggccaaccgt ttcgacgaag agcgttaggg
       61 gcaggcggta ggctgggtct gggttcaggc cgagatgttg accagcacgc ctgtcagttc
      121 gccagagacc tgacccaggg ccgcgccggc cgccgccgtc atcttgctgt tggtctgcgc
      181 caccgccagt tgcgtcaggt cgacgatcgg cgccgcagcc tggcccgagc tggcagggcg
      241 cggcggggcg taggggatgg cggcgttggc ggaaacttgc acggcgattg cctcgagaac
      301 tgccacacca ctctggttcg aaaaccttaa gcagccctga ccagacaggg ttaagggccg
      361 agcgctccgc cgtgacgcgg aacgaccccg cccctcagtc cggcctggaa gctactccgc
      421 agccaccgct tcggccggcc gccccacgcc catcgggcgc aggtccaggg cctggaacag
      481 ggcgctgtcc tcgtcctggc cgggcagggg ggtggtcagc agcttgccgc cgacaaagat
      541 cgaattggcg ccggccagga agcacagggc ctgcaactcg cggctcatgt cgtcgcgacc
      601 ggccgacaga cggaccatgg agcgggggca gacgatgcgg gcgaccgcga tcgtgcggac
      661 gaactcgatg gaatcgatgg ccttggcgcc gtccctcaga atcttgtcgc ctaacggcgt
      721 gccgctgacc ggcaccaggc cgttgatcgg caggctgtcg ggatggcttg gcagggtggc
      781 taactgatgg agcaggctgg cgcggtcgcg gcgcgtttcg cccatgccca caatgccgcc
      841 gcagcaggtg ctcatgccgg cgtcgcgcac ataggccagg gtgtcgaggc gctcctgata
      901 ggtgcgggtc gagaccacgt cgccgtaata ttccggcccg gtgtcgaggt tgtgattgta
      961 gtagtcgagg ccggcgtctt tcagctgttt ggcctggtcg gcggtcaaca tgccgagggt
     1021 ggcgcaggtc tccaggccca gggccttcac gcccccgatc atcgccgcca gcctgggcag
     1081 gtcgcggtcc ttcagctcgc gccaggccgc gcccatgcag aagcgctggg cgccgccgtc
     1141 gcgggccgcg cgcgcccggg cgatgacctc atcggcgtcc atcagcttct cggccttcag
     1201 gccggtcttg aagtgggccg actggctgca atagccgcag ttctcggcgc agccgccggt
     1261 cttgaccgac aacagctgcg acagctggac ctcggagggg tcgaagccgg cgcgatgaac
     1321 actggccgcc tggaacacca gttccatgaa cggcagctcg aacagggcct cgacctgggg
     1381 caaggtccag tcgtggcggg gcgcgttgtt ggcctggggg gcatgggtct gcgtcatggc
     1441 gcgacgtttg aaccgggtca tgcagcttgg caagatgcgg ccgtcacgcc ataggccatt
     1501 gcagccaaac cgcgcgcggt cggttaggtc tggggtaggg ccccgagggt tgagacggat
     1561 catggatcga gaaatcgagc tgaaattcct gatcgcgccc gaagcggccg accagattct
     1621 gtcgctgctg gacggcgaga gcgatgttcg acagctggac gccacctatt tcgacaccgt
     1681 cgatcacgcc ctgcgcaagg ccggctttgg tctgcgggtc cgtgacggcg agggtggccg
     1741 caagcagacc ctgaagtccg cctcggccgg cggggtgttt tcgcggggcg agtgggagac
     1801 gccgatcgcg gggcctggtc ccgaccagaa ggccctggcc gccacgccgg cggccgccgt
     1861 gctgaacggt caggccctgc agccggtgtt caccacccag gtcgagcgca tcgttcgcct
     1921 ggtccgccgc ggcgagacgg tgatcgaggc ggtcgtggac cggggcgcgc tgatcgccgg
     1981 gtcccgccgc gcggtggtgt gcgagctgga gcttgaactg aagtccggat cgccttcggc
     2041 gctgtttgag ctggctcgcg acgtggcccg tcaggttccg ttgcgtctgt cgctggtcag
     2101 caaggccgag cgcggctatg gcctggcgaa cgccgccgcc gggccgccgg gccgccgttc
     2161 ggcggttcgc ctggacccgg ccatgacggt ggaacaggcc ctgcacgccg ccgggcgcga
     2221 ggccctcacc catctgtgcg ccagcgccga tacactgcgc gaccgcccgg gacccgaggg
     2281 cgtgcaccaa ttgcgggtcg ctgtccgccg cacacggtcg ctgctgaaaa ccttcaagcc
     2341 gctggccgtc gacgcggccg cccggtcgct ggctctcgag ctcaaatggc tggctggcga
     2401 gctggatgcg gcgcgtaatc tggatgtctt cgtcgccgat gtttggcgcc cggcgtccga
     2461 agccgaaccc gcgcaggacc tggccgcttt cgggcgcgcc ctgctggggg ctcaggccca
     2521 ggcctatcgg cgcgtcgagg cggcgctgga aagtccccgg cagcgcgccc tgctgctgga
     2581 aacggccgcc tggctggagg ccggcgcctg gaccacggag ccggccctgg acgcggtgcg
     2641 ccatcgtcca gcagccgggt ttgcaggcga gatcctggac cacatgcgcc aggtcgtcat
     2701 cgagcgaggc gagtctctgg acgagatgga gcccgaagcc cggcaccggc tgcgcctgaa
     2761 gggcaagacc ctgcgctatg ccgccgagga tnnnnnnnnn ntcgcggccg ccaaggccct
     2821 gcaggacgcc ctgggcgacc ttaatgaccg cgcggcccgc gcccggctgg cgcgcgaggt
     2881 ggcgacggcc catggagaac cggaggccgc cttcgcggca ggccggctga ccgcctgcgg
     2941 cgatgacgcc gaggcgctgg gcagggcgcg aaaggcctat gcgactctcg ccgcggcaaa
     3001 ggtcttctgg tgaggcgatg tccgtgccca tccgtcacat aaagatatct ttatgcgtta
     3061 attgcccgga agggtccgtc gcggtataag ccgcgcaaga cattcatcga tcccaggagc
     3121 cgaccgtggc cgactacatc gtcaaggaca tctcgctcgc cgatttcggc cgcaaggaaa
     3181 tcgccatcgc cgagaccgaa atgcccggtc tgatggccac ccgcgccgaa tacggcccgg
     3241 cccaggtcct gaagggcgcc cgcatcgccg gcagcctgca catgaccatc cagaccgccg
     3301 tgctgatcga gaccctgacc gccctgggcg ccgaagtgcg ctgggcgtcg tgcaacatct
     3361 tctcgaccca ggaccacgcc gccgccgcca tcgccgcctc gggcgtgccg gtgttcgcct
     3421 tcaagggcga gaacctggtc gagtactggg aatacgccca caagatcttc gagtggcatg
     3481 acggcggcta tccgaacctg atcctcgacg acggcggcga cgccaccctg ctctgcgtgc
     3541 tgggccccaa ggccgagaag gacccgacgg tcctgaacaa tccgcagaac gaggaagaag
     3601 aagccctgtt cgccgtgatg aagaagtatc tggccgaaaa gccgggcttc tataccgcca
     3661 tccgcgacgc catcggcggc gtctccgaag aaaccaccac gggcgtccac cgcctgtacc
     3721 agatggccgc caagggcgag ctgccgttcc cggccatcaa cgtcaatgac agcgtcacca
     3781 agtccaagtt cgacaacctg tacggctgtc gggaatcgct ggtcgacgcc atccgtcgcg
     3841 gcaccgacgt gatgctgtcg ggcaaggtcg cggtggtctg cggctatggc gacgtgggca
     3901 agggttcggc cgcttcgctg cgccagggcg gcgcccgcgt gatcgtgacc gaaatcgatc
     3961 cgatctgcgc cctgcaggcg gcgatggaag gctatgaagt ccagaccctg gatgactgcg
     4021 ccggccgcgc cgacatcttc gtcaccgcca cgggcaacaa ggacgtgatc acggtcgatc
     4081 acatgcgggc catgcgcaac aatgcgatcg tctgcaacat cggccacttc gactcggaga
     4141 ttcaggtgtc cggcctgaag aacttcaagt gggacgagat caagccgcag gtccaccaca
     4201 tcgagttccc ggacggcaag aagatcatcc tgctgtcgga aggccgcctg gtgaacctgg
     4261 gcaatgcgac gggtcacccc tcgttcgtga tgtcggcctc gttcaccaac cagaccctgg
     4321 cccagatcga actgtggacc aacaagtcgg cctacaagaa cgacgtctac accctgccca
     4381 agcacctgga cgagaaggtc gccctgctgc acctggaaaa gctgggcgcc aagctctcca
     4441 agctgcgtcc cgaccaggcc gaatatatca acgtgccgga aaacggcccg ttcaagccgg
     4501 atcac
//
LOCUS       DAOMEO010000102         4503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2365_length_4503_cov_2.362635, whole genome shotgun sequence.
ACCESSION   DAOMEO010000102 DAOMEO010000000
VERSION     DAOMEO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2365_length_4503_cov_2.362635"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>285
                     /locus_tag="PLH31_03395"
     CDS             <1..>285
                     /locus_tag="PLH31_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR88309.1"
                     /translation="AAGVLGALTPVGHLDPKGWDMILSTNLTANWRLIRAMDPLLRAS
                     DAGRALFLTSSVAKSHRAFWGGYAASKAGLEAIVACYADEMENTPVRALCV"
     assembly_gap    286..295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(461..2236)
                     /gene="der"
                     /locus_tag="PLH31_03400"
     CDS             complement(461..2236)
                     /gene="der"
                     /locus_tag="PLH31_03400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQR88310.1"
                     /translation="MPLKLAIVGRPNVGKSTLFNRLAGKKLAIVDDQPGVTRDRRYAS
                     GRLGDLDLELIDTAGFEDVSDESLEARMRAQTELAVEEADVSLFIYDAREGVTPLDEV
                     FATLLRRRGKPVIIAANKAEGKAGQSGIGEAYKLGLGEPIPISGEHGEGMAELYAAML
                     AAFPEDQYSEEEDDDDKPIRIAIVGRPNAGKSTLVNRLIGEQRLLTGPEAGITRDSIS
                     VDWLWDGRKVRLVDTAGLRKKAKVQEKLEKLSTQDSIRAVTFAEVVLLVMDATHPFEV
                     QDLQIADLTEREGRALVFVLAKWDLVEDPVAQLKDFREHAERMLPQLRGAPVVALSGE
                     TGKGIEKLMPAVMKIHRDWATKVKTRDLNDWLQMAMQRHPPPAVSGKRVKPKYMAQIK
                     ARPPTFVLFSTRADQMPEHYRRYLINSLRESFDLPGVPLRITIKSGANPYADGEAKGH
                     SGRGKREFERREREEERIRASRNTVKKSKRIADEAAAAAAVAAGLPPPEKAPVRGPKK
                     PVKAEAKTTSSAVTAPVAEPGKAAVKSVKKQGPRAQKKVSTTPSYKVGFKAAGGKAAS
                     GPKRPVAGSSIVRGNKGPGKAKPKR"
     gene            complement(2359..3786)
                     /locus_tag="PLH31_03405"
     CDS             complement(2359..3786)
                     /locus_tag="PLH31_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQR88311.1"
                     /translation="MSRPMNSKRSVALIALVSAAVTLSGCSTVSKLNPFDKSEKDKAQ
                     ATQGQRISVIAFDQKVEAAEALKGTEFYLPEPSAQAEWRLPGGNAEQSVEHVDAGKAF
                     EIAWRKGFGQKANRKFHITAPPVATADRIFVMDGESTVSALDAKTGSVDWRKDLRPSA
                     GPGKGGFFGFGGAKNDRLGFGGGLALSPDNKLIVASGFRFVAQLDAATGVEAWRQGSS
                     TPIHAAPTVVDGRVFVVSTDNELLTYNAATGAPGWTYQALIEPARILSAPSVAVSGDT
                     AVAGFASGELVALRAANGNDLWSEALSRASRTNALSEIRDIPGRPVIYKGDVFAVSHS
                     GVFSAIDLRTGQSRWSLPITGITSPWAAGDVVYVVDKSGQVICVARESGAIYWIRDLN
                     EGLGQTKRQKKKLAKRPRLWSTPILASGRLITVSSTGEAVALNPKTGATEKTLKIGAP
                     VLLAPIAVGDMIYLVTDDAQVVAIR"
     assembly_gap    3861..3870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3871..>4503)
                     /locus_tag="PLH31_03410"
     CDS             complement(<3871..>4503)
                     /locus_tag="PLH31_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR88312.1"
                     /translation="SDRYKTLALKALPWIGAATVAVVIGVGGYWGWTAYQTKEAAKAS
                     EAYQSALETLGRDGPAKGFSAMEAVSKGGAAGYRVLALMHMGGVRLDEGKTADAVRYF
                     DEAAKLSSDPVIGDLARLKSALALMDTASFKDTEARLTPLAGEKSPYRVNAKEALAFA
                     KLQAGDLKGAREDFSVLSNLLDNNSEGVRQRAQAAMQMIDSGSAKDLAAVV"
BASE COUNT          697 a         1478 c         1505 g          803 t
ORIGIN      
        1 gccgccggtg ttctgggcgc gctgacgccc gtgggccacc tcgaccccaa gggttgggac
       61 atgatcctgt cgaccaacct gaccgccaac tggcgactga tccgcgccat ggacccgctg
      121 ctgcgggcct ccgacgcggg ccgggccctg ttcctgacca gcagcgtcgc caagagccac
      181 cgcgccttct ggggcggcta tgcggcgtcc aaagccggcc tggaagccat tgttgcctgc
      241 tatgccgacg agatggagaa caccccggtt cgcgccctgt gcgtcnnnnn nnnnntttcc
      301 cgggcgagga tccgacgacg gttccggccc cggagaccat cgtgccctac attatcgatc
      361 ttgtccggcc cgaccgcgaa ccgccccggg gcgtcgtgcg attcaaggag cgcggccagg
      421 agtcggccag cgtaggctga ctcctggcat ctagcctcgg ctagcgcttg ggcttggcct
      481 tccccggccc cttgtttcca cgcacgatgc ttgaacctgc gacagggcgc ttgggtcccg
      541 aggccgcctt gccgccggcg gccttgaagc cgaccttgta gctgggtgtg gtcgagacct
      601 ttttctgggc gcgaggcccc tgcttcttga ccgacttcac ggcggccttg ccgggctcgg
      661 cgacgggcgc cgtgacggcc gaactggtgg tcttggcctc ggcctttaca ggcttcttgg
      721 ggccccggac cggagccttc tccggaggcg gcaggccagc cgcaaccgca gcggcggcgg
      781 cggcctcgtc ggcgatgcgc ttggacttct tgaccgtgtt gcgcgaggcg cggatgcgct
      841 cttcctcgcg ttcgcggcgt tcgaactcgc gcttgccccg gccgctgtgg cccttggcct
      901 cgccgtcggc ataggggttg gcgcccgact tgatggtgat gcgcaacggc acccccggca
      961 ggtcgaagct ctcgcgcagg ctgttgatca gatagcgccg atagtgctcg ggcatctggt
     1021 cggcgcgggt tgagaacagg acgaaggtcg gcggacgcgc cttgatctgc gccatgtact
     1081 tgggcttgac gcgcttgccg ctgacggcgg gcggcgggtg cctctgcatc gccatctgca
     1141 gccagtcgtt caggtcgcgg gtcttgacct tggtcgccca gtcgcggtgg atcttcatca
     1201 ccgccggcat cagtttttcg atgcctttgc cggtctcgcc cgacagcgcg accacgggcg
     1261 cgccgcgcag ctgcggcagc atgcgctcgg cgtgttcgcg gaaatccttc agctgagcga
     1321 cggggtcctc gaccaggtcc cacttggcca ggacgaacac cagcgcccga ccttcccgct
     1381 cggtcaggtc ggcgatctgc agatcctgaa cctcgaacgg atgggtggcg tccatcacca
     1441 gcagcaccac ctcggcgaag gtgactgcgc ggatcgaatc ctgggtggag agcttttcca
     1501 gcttctcctg caccttggcc ttcttgcgca ggccagcggt gtcgaccaga cggaccttgc
     1561 ggccgtccca cagccagtcg accgagatcg agtcgcgcgt gatgccggcc tcggggccag
     1621 tcagcaggcg ttgctcgccg atcaggcgat tgaccagggt ggacttgccg gcgttggggc
     1681 ggccgacgat ggcgatgcgg atcggcttgt cgtcgtcgtc ctcttcctcg gaatactgat
     1741 cctcggggaa ggccgccagc atggcggcgt acagctcggc catgccttcg ccatgctcgc
     1801 ccgagatcgg gatcggctcg ccaagaccca gcttgtaggc ctccccgata ccggattgac
     1861 cggctttgcc ctcggccttg ttggcggcga tgatcaccgg cttgccgcga cggcgcagca
     1921 gggtggcgaa cacctcgtcc agcggcgtga cgccttcgcg ggcgtcataa atgaacaacg
     1981 agacgtcggc ttcctcgacg gccagctcgg tctgggcgcg catccgcgct tccaggctct
     2041 cgtccgagac atcctcgaag ccggcggtgt cgatcagttc caggtccagg tcgccgaggc
     2101 gaccagaggc atagcggcgg tcacgggtca ccccgggctg gtcatcgaca atggccagtt
     2161 tcttaccggc gagacgattg aacagcgtgg acttgcccac attggggcgt ccgacaattg
     2221 cgagtttcaa aggcatgggc ttgcagtagt cgatttttgc cgttcccgca aagcgaagac
     2281 ccgggccggc tttcgccgac ccgggtctcg ggaccgttgg tgtgaccgtc ctcgggccta
     2341 aagcccgtgg cgtcgcgtct agcgaatggc tacgacctgg gcgtcgtcgg tgaccagata
     2401 gatcatgtcg ccaaccgcga tcggcgccag aagcaccggc gcaccgatct tcagggtctt
     2461 ttcggtcgcg ccggtcttgg gattcagcgc cacagcctcg ccggtgctgg aaaccgtgat
     2521 cagacggccc gaggccagga tcggggtcga ccagaggcgc ggacgcttgg ccagcttctt
     2581 cttttgacgc ttggtctggc ccagaccttc gttgagatcg cggatccagt agatcgcgcc
     2641 gctctcgcgg gcgacgcaga tgacctggcc ggatttgtcg acaacataga cgacgtcgcc
     2701 ggcggcccag gggctggtga tgccggtgat cggcaggctc cagcgcgact ggccggtgcg
     2761 caggtcgatg gccgaaaaca cgcccgagtg gctgacggcg aagacatcgc ccttgtagat
     2821 gaccggccgg ccgggaatgt cgcgaatctc ggacagcgca ttggtgcggc tggcgcgcga
     2881 cagggcctcg ctccacagat cgttgccatt ggcggcgcgc agggcgacca gttcacccga
     2941 ggcgaaaccc gccacggcgg tgtcgccgct gacggcgacg ctgggcgccg acaggatacg
     3001 cgccggctcg atcagggcct gataggtcca gccaggggcg cccgtggcgg cgttgtacgt
     3061 cagcagttcg ttgtcggtcg aaacgacgaa cacgcgacca tcgacaaccg tcggggcggc
     3121 atggatcggg gtcgaacttc cctggcgcca cgcctcaacg cccgtcgcgg cgtcgagctg
     3181 ggccacgaaa cggaagccgg acgcgacgat cagcttgttg tcggggctga gcgccaggcc
     3241 gccaccaaag cccaggcggt cgttcttggc gccgccaaag ccgaagaagc cgcccttgcc
     3301 cgggcctgcc gacggacgca gatccttgcg ccaatccacc gagcccgtct tggcgtcgag
     3361 cgccgagacc gtggactcgc cgtccatgac aaagatccgg tcagcggtcg ccactggcgg
     3421 ggccgtgatg tggaacttgc ggttggcctt ttggccaaaa cccttgcgcc aggcgatctc
     3481 gaaggccttg ccggcatcga catgctcgac cgactgctcg gcattgccgc ccggcaaacg
     3541 ccattcggcc tgggcgctcg gttcgggcag atagaactcg gtgcccttca gggcttcggc
     3601 ggcctccacc ttttgatcaa aggcgatgac cgagatccgc tgcccctggg tggcctgggc
     3661 cttgtccttt tcggacttgt cgaaggggtt gagcttggag acggtggagc agccgctcag
     3721 cgtcaccgcc gcagacacca gcgcgatcaa cgccacggaa cgcttggaat tcatggggcg
     3781 gctcattggg cgctgttcgg cgcgggttgc ggagcgatcg gcatggcagc cgacggcggc
     3841 agggcgatcg aggctttgac nnnnnnnnnn acgacagcgg ccagatcttt ggccgagccg
     3901 gaatcaatca tctgcatcgc cgcctgggcg cgttggcgaa cgccttcgga attgttgtcg
     3961 agcaggttgg acagaaccga aaagtcctcg cgggcgccct tgaggtcgcc ggcctgaagc
     4021 ttggcgaacg ccagcgcttc ctttgcgttc acgcgatatg ggctcttttc cccggccaac
     4081 ggcgtcaacc tcgcttcggt atccttgaac gaggccgtat ccatgagcgc caaggcggat
     4141 ttgaggcgcg ccagatcgcc gatcacggga tcggaggaaa gcttggccgc ttcgtcgaaa
     4201 tagcgcacgg cgtcagccgt cttgccctcg tcgaggcgga caccgcccat gtgcatcaac
     4261 gccagaacgc gatagcccgc ggctccgccc ttggagacag cttccatggc ggaaaagccc
     4321 ttggcggggc catcgcggcc cagggtctcc agcgcagact gataggcttc cgaagccttg
     4381 gcagcttcct tggtctgata ggcggtccag ccccaatagc cgccgacgcc gatgacaacc
     4441 gccacggtcg cggcgccgat ccacggcagc gccttcagcg cgagggtctt gtaacggtcg
     4501 gag
//
LOCUS       DAOMEO010000103         4495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2371_length_4495_cov_2.443919, whole genome shotgun sequence.
ACCESSION   DAOMEO010000103 DAOMEO010000000
VERSION     DAOMEO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_4495_cov_2.443919"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="mdoH"
                     /locus_tag="PLH31_03415"
     CDS             complement(<1..1044)
                     /gene="mdoH"
                     /locus_tag="PLH31_03415"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HQR88313.1"
                     /translation="MSDRSSLFDLRTSTSVSLDQVIQRRDTVEIPAEAPLAMPVQPLA
                     FWQGGARRATEVVANITLRRTIVFLATLVMGAAGWKATFDTIALGGVTRLEFICLALL
                     APLFLALSLWFCTAVAGFVILLGKPRDPLGIDSAKPMPKLRARTAILMPVHNEDATAV
                     FARLRAMDASIAETGNSRAFDIFILSDTREPAVALAEQACFARFRREANSAVFYRVRQ
                     ENKGRKAGNIADWVARWGSAYEHMLVLDADSLMTGDAMVRLADAMERHPGAGLIQTMP
                     MLINGQTIFARTLQFATRLYGRVAWTGLAWWSGSESSFWGHNAIVRTRAFAETCGLPS
                     LDGPKPFGGEVMSH"
     assembly_gap    1105..1114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1115..1636
                     /locus_tag="PLH31_03420"
     CDS             <1115..1636
                     /locus_tag="PLH31_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HQR88314.1"
                     /translation="LGAFMAVDFRRGEPSASPRVKLRSRNRYQKSRLSRTCGKKLGIR
                     RGRVAVAPMENLGNVMKTKLIISIFVLSALAACGPGPQTSTAEKPGNASAAATAALPT
                     YDSTGVISAVNGLTLTLDHDGASAAGVPAGRNDFQTYADVVADAPLTPGVRVAFKFRK
                     ADTGLQLVELKAR"
     gene            complement(1688..2716)
                     /locus_tag="PLH31_03425"
     CDS             complement(1688..2716)
                     /locus_tag="PLH31_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HQR88315.1"
                     /translation="MTVSREIRLKSRPVGVPVHSDFELASVELAAPGPGEVQVKNLWM
                     SVDPYMRGRMYDRPSYVPPFELGKALQGGAIGEVVASNDPDFAVGDIVNSMFGWREAF
                     NISPSAMAAAGPMGALTKIDTHGLPPQAFLGVLGMPGMTAYAGLLRVAALKDGDVVFV
                     SAAAGAVGSVVCQIAKLKGHTVIGSAGGPEKVAFLKSIGVDHVIDYKATPDVVAELAK
                     VAPKGIDVYFENVGGVHLEAAINSARPFARMALCGMISQYNETGKPTGPANIILAVGK
                     SLRLEGFIVSNHYDLYPAFARDMAEWIKAGKITWKETVEDGVENAPEAFIKLFAGENM
                     GKMLVKLA"
     gene            2837..3775
                     /locus_tag="PLH31_03430"
     CDS             2837..3775
                     /locus_tag="PLH31_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR88316.1"
                     /translation="MTQAILKAGVVGAGVFGGYHAKKYVELPGVDLVAVFDIDLARAK
                     ALAEPLGATAHDDMAAFLAAVDVVTVATPAVHHARAALAALKAGKPVYCEKPLAVTPQ
                     DADALVAAAMKAGVFLACGHQERVVFQAMGLLDIPEQPLRLEAVRRGTPSDRNLDVSV
                     VLDLMIHDIDLALALCAADPIAVEGEGAITRSESLAHSPSLDWVKTEATFANGFTAVF
                     DASRVAEARERTMKVVYPSGEVEIDFLARTFRNTTPFPLIEDFTDTPAGKDPLGASVA
                     GFLAAVRGESPRPVVTGEEAARALDLALAVEQAAGG"
     gene            complement(3778..4056)
                     /locus_tag="PLH31_03435"
     CDS             complement(3778..4056)
                     /locus_tag="PLH31_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR88317.1"
                     /translation="MRWTSSARLDRLDILDHIATDSPRAAARMDALFAEAAETLAAFP
                     QKGRLGKIAGTRELIPHESYRLVYEIADDVVWVLALVHAARQWPPERG"
     gene            complement(4059..4352)
                     /locus_tag="PLH31_03440"
     CDS             complement(4059..4352)
                     /locus_tag="PLH31_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HQR88318.1"
                     /translation="MSKDAVFTLKLEAQLRDAFVAEAQASHRPASQIVRDLMRDFVER
                     QRQARLHDEFLRRKVEIARAERLSGLTDSDENVEAEFAARRAELLRKADEAAA"
BASE COUNT          739 a         1467 c         1536 g          743 t
ORIGIN      
        1 gtggctcatc acttcgccgc cgaagggctt gggaccgtcc agcgagggca ggccgcaggt
       61 ctctgcgaag gcgcgcgtgc ggacgatggc gttgtggccc cagaaggagc tctcgctgcc
      121 cgaccaccag gccagacccg tccaggcgac gcggccatac aggcgcgtgg cgaactgcag
      181 ggtgcgggcg aagatcgtct ggccgttgat cagcatcggc atggtctgga tcagacccgc
      241 gccggggtga cgttccatgg cgtcggccag gcgcaccatg gcgtcgccgg tcatcaggct
      301 gtcggcgtcc aggaccagca tatgctcgta ggcgctgccc cagcgggcca cccagtcagc
      361 aatgttgccg gccttgcggc ccttgttttc ctggcggacg cgatagaaca cggccgaatt
      421 ggcctcgcgg cggaagcggg cgaagcaggc ctgctcggcc aaggccacgg cgggctcgcg
      481 cgtgtcgctg aggatgaaga tgtcgaaggc gcggctgttg cccgtttcgg cgatcgaggc
      541 gtccatggcg cgcaggcggg cgaagacggc ggtggcgtcc tcgttgtgca cgggcatcag
      601 gatggcggtg cgggcccgca gcttgggcat gggcttggcg ctgtcgatgc ccagcggatc
      661 cctgggcttg cccagcagga tcacgaagcc ggccacggcg gtgcagaacc acagcgacag
      721 ggccaggaac agcggcgcca gcagggccag gcagatgaat tcaagacggg tcacgccgcc
      781 cagggcgatg gtgtcgaagg tggccttcca gccggccgcg cccataacga gggtcgcaag
      841 gaacacgatc gtgcggcgaa gggtgatgtt ggcgaccacc tcggtggcgc gtcgcgcgcc
      901 gccctgccag aacgccagcg gctgcaccgg catggccagc ggcgcctcgg cggggatttc
      961 caccgtatcg cggcgctgga tgacctgatc gagggagacg ctcgtcgagg tgcgcaggtc
     1021 gaagagggag gagcgatcgc tcatgtcgtc atcgtctcgg tctgtccggc cggggccggg
     1081 ctctctccgg cgcatcgccg catcnnnnnn nnnnctagga gcgttcatgg cggtcgattt
     1141 ccgacgtgga gagccgtcgg cgtcacctag agtaaaactt cgatcacgaa atcgctacca
     1201 gaaatcacgc ttatctcgca cttgcggcaa aaaacttggc atacgccgtg ggagagtggc
     1261 ggtcgcgcca atggagaatt tgggtaacgt catgaaaacc aagctgatta tctcgatttt
     1321 cgtcctgtcg gcgctggccg cctgcggtcc tggcccccag acttcgacag cggaaaagcc
     1381 cggcaacgcg tccgcggcgg ccactgcggc cctgccaacc tacgattcga cgggcgtgat
     1441 ttccgccgtg aacggcctga ctctcacgct cgatcacgac ggcgcgagcg cggcgggcgt
     1501 gccggccggc cgcaatgact tccagaccta cgccgatgtg gtggccgacg cgccgctgac
     1561 gccgggcgtt cgcgtggcgt tcaagttccg caaggccgac accggcctgc agctggtgga
     1621 actgaaggcc cgctgatccg aacgcgcccc ctccgccacg cggaggaggg ggcgatcatc
     1681 ggctggatca ggccagcttg accagcatct tgcccatgtt ctcgccggcg aacagcttga
     1741 tgaaggcctc gggagcgttc tcgacaccgt cctcgacggt ctccttccag gtgatcttcc
     1801 cggccttgat ccactcggcc atgtccctgg cgaaggccgg atagaggtcg tagtggttgg
     1861 agacgatgaa gccctccagg cgcaggctct tgcccaccgc caggatgatg ttggccgggc
     1921 cggtcggctt gccggtttcg ttatattgcg agatcatccc gcacagcgcc atgcgggcga
     1981 aggggcgggc cgaattgatg gccgcctcca ggtgcacgcc gcccacattc tcgaaataga
     2041 cgtcgatgcc cttgggcgcg accttggcca gttcggccac cacgtcgggc gtagccttgt
     2101 agtcgatgac gtgatcgacg ccgatcgact tcaggaacgc gaccttttcc ggtccgccgg
     2161 ccgagccgat cacggtgtgg cccttcagct tggcgatctg gcagaccacc gagcccacgg
     2221 cgccggcggc ggccgagacg aacaccacgt cgccgtcctt cagggccgcc acgcgcagca
     2281 ggcccgcata ggcggtcatg ccgggcatgc ccagcacgcc caggaaggcc tggggcggca
     2341 ggccatgggt gtcgatcttg gtcagggctc ccatcgggcc ggcggccgcc atcgccgagg
     2401 gcgagatgtt gaaggcttcg cgccagccga acatcgagtt gacgatatcg cccacggcga
     2461 agtccggatc gttggaggcg accacctcac cgatcgcccc gccctgcagg gccttgccca
     2521 gctcgaacgg cggcacatag ctggggcggt catacatgcg gccgcgcatg taggggtcga
     2581 ccgacatcca cagattcttg acctgcactt cgcccggacc gggcgcggcc agctccaccg
     2641 aggccagttc gaagtcggaa tggacgggga cgccgacggg gcggctcttg aggcggattt
     2701 cgcgcgagac ggtcatgggt gttctcccta attcacgcac ctggggcgcg tttctaattt
     2761 aaacgcttgt ttagcgcgtt gcggcgccgg gctccagtgc ttccgggcga aggcgaagcg
     2821 gtttagacaa cggcccatga cccaagcgat cctcaaggca ggcgttgtcg gcgccggcgt
     2881 gttcggcggc tatcacgcca agaagtatgt cgagctgccc ggcgtcgatc tggtcgccgt
     2941 gttcgacatc gacctggcgc gggccaaagc cctggccgaa ccgctgggcg cgacggccca
     3001 tgacgacatg gccgccttcc tggccgccgt cgacgtggtc accgtcgcca cgcccgccgt
     3061 ccaccacgcc agggcggcgc tggcggcgct gaaggcgggc aagccggtct actgcgaaaa
     3121 gcccctggcc gtcacgccgc aggacgccga cgccctggtc gccgccgcga tgaaggctgg
     3181 cgtgttcctg gcctgcggtc accaggagcg ggtggtgttc caggccatgg gcctgctgga
     3241 catccccgag cagcccctgc gcctggaagc ggtgcggcgt ggcacccctt cggatcgcaa
     3301 tctcgacgtc tcggtggtgc tggacctgat gatccacgac atcgatctgg ccctggccct
     3361 ctgcgccgcc gaccccatcg ccgtggaagg cgagggggcg atcacccgct cggagtcgct
     3421 ggcccattct ccctcgctgg actgggtgaa gaccgaagcc acatttgcca atggcttcac
     3481 cgccgtgttc gacgcctccc gcgtcgccga agcccgcgag cggaccatga aggtcgtcta
     3541 cccctccggc gaggtcgaga tcgacttcct ggcccgcacc ttccgcaaca ccaccccgtt
     3601 cccgctgatc gaggacttca ccgacactcc ggccggcaag gatccgctcg gcgccagcgt
     3661 cgccggcttc ctggcggcgg tgcgcggcga gagcccgcgt ccggtggtga ccggcgaaga
     3721 ggccgcccgg gcgctggacc tggcgctggc ggtggagcag gcggcggggg gctgagctca
     3781 gccgcgttcc ggcggccatt ggcgggcggc atggacaagg gcgaggaccc acacaacgtc
     3841 gtccgcgatt tcatagacga gccgatagct ctcgtgaggg atcaactccc tcgtaccggc
     3901 gatctttccc aagcgccctt tttggggaaa ggccgccaag gtttctgcgg cctcggcgaa
     3961 cagcgcatcc atccgggcgg cggctcgcgg gctatcggtg gcgatgtggt ccaggatgtc
     4021 gagacggtct agccgcgccg acgacgtcca cctcaccttc acgcggcggc ctcgtccgcc
     4081 tttctcagca gttcagcgcg gcgcgcggcg aactcagctt caacgttctc gtcggagtca
     4141 gtcaggccag acagacgttc ggcccgagcg atctccacct tgcggcgcag gaactcgtcg
     4201 tggagccgcg cttggcgttg acgctcgacg aaatcacgca tgaggtcgcg gacgatctgc
     4261 gaggcggggc ggtgactggc ctgcgcctcg gcgacgaagg cgtcgcggag ttgggcttcg
     4321 agcttgagcg tgaaaacggc atccttggac atggtgagct ttcggacaga ggtgcatacg
     4381 acgtatatac gttttcatta ccaggggaaa tgagcacgtc aacgaaacgc ctgtcgacaa
     4441 ggactccttg ggccagagcg cgagcgcgat tgacggttca atttgtctga gtaat
//
LOCUS       DAOMEO010000104         4476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2382_length_4476_cov_2.646460, whole genome shotgun sequence.
ACCESSION   DAOMEO010000104 DAOMEO010000000
VERSION     DAOMEO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2382_length_4476_cov_2.646460"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLH31_03445"
     CDS             complement(<1..767)
                     /locus_tag="PLH31_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88319.1"
                     /translation="MTDALHSAPAPRCGLPKVPENSLIASVLLSFLATAGLFYVNIMA
                     ALVDGLIGGVGLTAKQAGLVASANVYGAAVGALTAVFIIRRLPWRPTAVTVLLGLIAI
                     DLVSTLLTTPALLIAVRFAHGLIGGLLVGVAFAVIARTKTPDRTFGMLLVVQFGLGGL
                     GLMFLPRLSPVFGPGVLFAALAAFSLVTLAMVPFLGAYKAAPKVVTEGARSPPPWIPL
                     SLVFTAIFLFQAANMGLAAYIIGLGKSAGLELKFITDT"
     gene            complement(764..>2507)
                     /locus_tag="PLH31_03450"
     CDS             complement(764..>2507)
                     /locus_tag="PLH31_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88320.1"
                     /translation="LIRVRAVGGEQASCWADAVTVTGERPAAGGPRAVLTLESLMTGP
                     GLTAPVDLSAFGAPADAEALTGRFAARLALDLTSVTLGMSLVRDETGDTAAERAARVT
                     LPAFDFLFVRDDGDLVPVRRGVIVGDHPAWDWVIEPGRVWREAGDLGWLRAAVPFALQ
                     ERNANCLHNGVLTFLFKPDGSVSKVAMQISSETCAYLKFDAWATVPARLTPSAVVEAD
                     AVITAWRAEVAARLPVRPLADLARLRPDVNLAAFSLKAPKDGDPPTAFGLVVEGVHYA
                     SPCQTRHGDNPYCDVLDLPSYSTAKSIVGGVGLMRLEALHPGSGGALIRDHVPACARS
                     DAWSGVTLAHTLDMASGLYGSQVFEADENAPAGQVFFDVEDHAAKAAYACGQFPRRVR
                     PGTTFVYRTSDTYLLGTAMADILRQAGEGDLYDDLIAPLWRALGLGQTIMATRRTHDA
                     ARQPFTGWGLTYHRDDILRIASWLGDGGVIDGRRVLDPALLAAALQRDPVQPGLPAGG
                     PTYRYKAGFWARNISGALNCSQPVWTPFMSGFGGISVVLLPGGVTYYYFGDSGVYDWA
                     PAAVEAGRIRNLCA"
     assembly_gap    2508..2517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2518..2895)
                     /locus_tag="PLH31_03455"
     CDS             complement(<2518..2895)
                     /locus_tag="PLH31_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88321.1"
                     /translation="MRPIILAATALAAWLCAVAPVLAGPVFVDDFQDGKADGWSATGS
                     GDVRLTTYGDNISLRVTGGATAATTVSTRGFVRVSVAGSLAAMGLVRTDACLIEVSPD
                     AGANWQTVVAVRDGEDDGVTLKRA"
     gene            complement(2895..>3089)
                     /locus_tag="PLH31_03460"
     CDS             complement(2895..>3089)
                     /locus_tag="PLH31_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88322.1"
                     /translation="CGLAWQDSCLRFHENSSPSATASAVQVRQPIHSQSVGLWRRYAE
                     QLGPLRAALTAGGIDPDELL"
     assembly_gap    3090..3099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3100..4251)
                     /locus_tag="PLH31_03465"
     CDS             complement(<3100..4251)
                     /locus_tag="PLH31_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR88323.1"
                     /translation="MDELGRVAPLIRDGRVGDALARLAALEARAGDDARLLQHLGEHY
                     THCARSADAARCYRRAAALAPDDPRGLYNLATALIALGQAGEAEALLDRVIQLDPEDY
                     DAWQNRSTLRRQTPGSNHTAALLQASRKRLTGPGEVAISYALAKEFEDLGEYEESFAW
                     LRRGAERRRSLLSYRVEADVAAMAEIERVFHAALFTQPSKGCDAAGPIFVLGLPRSGT
                     TLVDRIASSHTDVESLGEINDFALALMRLAGPAKGKGELIQRSADLDPAALGRAYLDS
                     VAGYGRTTPLFIDKTPANFLYIGLIARALPNARIIHLRRHPMDSGYALYKTLFRMGCP
                     YSYDLADIAAYQLAHRRLMDHWRDVLPGRILDIDYEGLVTDAETISRRII"
     gene            complement(4289..>4476)
                     /locus_tag="PLH31_03470"
     CDS             complement(4289..>4476)
                     /locus_tag="PLH31_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88324.1"
                     /translation="LAKGPWAATLFVKNILNEDGVTGEFTEAYMGTRPAVGYYGNASK
                     QFISLPRTLGLSLDYSF"
BASE COUNT          832 a         1496 c         1478 g          650 t
ORIGIN      
        1 gtgtcggtga tgaatttgag ctctagtccg gcgctcttgc ccaaaccaat gatgtaggcg
       61 gccagcccca tattggcagc ctgaaacagg aagatcgccg tgaacaccag gctcagggga
      121 atccaggggg gcggcgacct ggcgccttcg gtgacgactt tcggcgcggc tttgtaggct
      181 cccagaaatg gaaccatcgc cagcgtcacc aggctgaatg ccgccagggc ggcaaaaagc
      241 acccccggac cgaacaccgg cgacaatcgg ggcaggaaca tcaggcccag tccccccagt
      301 ccgaactgca cgaccagcaa catgccgaag gtgcggtcag gggtctttgt ccgggcgata
      361 accgcgaagg cgaccccgac caacagacct ccgatcaggc cgtgcgcgaa ccgcacggcg
      421 atcagcagcg cgggcgtcgt cagcagggtc gaaaccagat caatggcgat caggcccagc
      481 aggaccgtca cggccgtggg ccgccagggc agtctgcgga tgatgaacac ggccgtcagc
      541 gcgcctacgg ccgcgccata gacattggcc gaagcaacca gtcccgcctg tttggcggtc
      601 aggcccaccc cgccgatgag accatcgacc agggcggcca tgatgttgac gtagaaaagg
      661 ccggcggtcg ccaggaagga cagcagcacg ctcgcgatca ggctgttctc aggaaccttc
      721 ggcaggccac agcgcggcgc cggcgcagag tgaagggcgt cggtcatgcg cacaaattcc
      781 tgatcctgcc ggcttcgacg gcggccggag cccagtcata aacgccgctg tcgccgaaat
      841 agtagtaggt caccccgccc ggcagcagca cgacggagat gccgccgaac cctgacatga
      901 acggcgtcca gaccggttgc gagcagttca gcgcaccgct gatgttccgg gcccagaatc
      961 cggccttgta gcgataggtc gggccgccgg ccggcaggcc cggctggacc ggatcccttt
     1021 gaagcgccgc cgcgaggagc gccggatcaa gcacccgccg cccgtcaatc acaccgccgt
     1081 ctcccaacca gctggcgatg cgcaggatgt cgtcgcgatg ataggtaaga ccccagccgg
     1141 tgaagggctg gcgcgccgca tcatgggtgc gacgggttgc catgatggtc tggcccagcc
     1201 cgagcgcgcg ccagagcggg gcgatcaggt cgtcatagag atcgccctcc cccgcctgcc
     1261 gaagaatgtc ggccatggcc gtccccagca ggtaggtgtc tgaggtgcga tagacgaagg
     1321 tcgttccggg gcggacgcgg cgagggaact ggccgcaggc ataggcggcc tttgcggcgt
     1381 ggtcttccac gtcgaagaag acctgcccgg ccggcgcgtt ctcgtcggcc tcgaagacct
     1441 gcgagccgta gaggcccgag gccatgtcaa gggtgtgggc cagagtcacg ccgctccagg
     1501 cgtcgcttcg ggcgcaggcg ggaacatggt cacggatcaa cgccccgccg ctgcccggat
     1561 gcagcgcttc cagccgcatc aggccgacgc cgccgacgat cgacttagcc gtcgaatagg
     1621 acgggaggtc aaggacatcg caataggggt tgtcgccgtg tcgggtctga caggggctcg
     1681 catagtgaac accctcgacg acaagaccaa aggccgtcgg cggatcgccg tccttgggcg
     1741 ccttcagcga aaaggcggcg aggttcacgt ccgggcgcag tcgggctagg tcggcgagag
     1801 gacgcactgg aaggcgcgcg gcgacctcgg cgcgccaggc tgtgatgacc gcgtcggcct
     1861 ccacaaccgc cgaaggcgtc aggcgcgccg ggaccgtggc ccaggcgtcg aacttcagat
     1921 aggcgcaggt ttccgaagag atctgcatcg ccactttgga aaccgatccg tcgggcttga
     1981 acaggaaggt cagcacgccg ttgtgcaggc agttggcgtt acgctcctgg agcgcaaacg
     2041 gaacggccgc ccgcagccat cccaagtcgc cggcttcgcg ccagacccgc cccggctcta
     2101 tcacccaatc ccaggcgggg tgatcgccaa cgatcacccc gcgcctgacc ggaaccaggt
     2161 ccccatcgtc acgcacaaag aggaaatcga acgccggcaa ggtgacccgg gccgctcgct
     2221 cggcggcggt gtcgcccgtc tcatcgcgga ctaggctcat gcccagggtg acgctggtca
     2281 gatccagggc cagtcgcgcc gcgaaccgcc ctgtcagcgc ctcggcgtcc gctggcgcgc
     2341 cgaaggccga aagatcgacc ggcgccgtca ggccggggcc ggtcatcagg ctttccaggg
     2401 tcaagacggc gcgcgggccg ccggctgcgg gacgctcgcc ggtcacagtc accgcatccg
     2461 cccagcagga cgcttgctcc ccgccgacgg cccgaacccg gatcaggnnn nnnnnnnagc
     2521 ccgtttgagc gtcacgccgt catcctcccc atcacggacc gcgacgaccg tctgccagtt
     2581 tgcgccggcg tcgggcgaga cctcgatcag gcaggcgtcc gtccgaacca ggcccatcgc
     2641 ggcgagcgag ccggcgacgg acacccgcac gaagccgcgc gtggaaaccg ttgtcgcggc
     2701 cgtcgctccg ccggtcacgc gaagcgagat gttgtcgcca taggtggtca gacgcacgtc
     2761 gccggagccg gtcgcgctcc agccatcggc cttgccgtcc tggaaatcat cgacaaagac
     2821 cgggccggcc agaaccggcg caaccgcgca gagccatgcc gccagggcgg tcgctgcaag
     2881 gataataggg cgcatcaaag caactcgtcg ggatcaatgc cgccagccgt caaggccgct
     2941 ctcagaggac caagttgctc ggcatagcgc cgccagagtc cgaccgactg gctatggatg
     3001 ggctgacgca cctggaccgc actggctgtc gccgacgggc tgctgttctc atggaagcgc
     3061 agacagctgt cctgccaagc cagaccgcan nnnnnnnnna atgatccgtc ggctgatggt
     3121 ctctgcgtcg gtgactagac cctcgtagtc gatgtcgagg atgcggcccg gcaagacgtc
     3181 acgccaatgg tccatcaaac gccggtgcgc caactgatag gcggcgatgt cggcgagatc
     3241 atacgaatag gggcacccca tccggaacag cgtcttgtag agcgcatagc cactgtccat
     3301 cgggtgacgg cgcaggtgga tgatccgggc gttcgggagc gcccgcgcga tcagcccgat
     3361 gtagagaaaa ttggccgggg tcttgtcgat gaacagcggc gtggtccggc catagccggc
     3421 gacgctgtcc agataggccc gcccaagagc ggccgggtcc agatcggcgg aacgctggat
     3481 gagctcgccc ttgcccttgg cgggaccggc caatcgcatc agagccagcg cgaaatcatt
     3541 gatttcgccc aagctctcga catccgtgtg ggagctggcg atgcgatcca ccagggtcgt
     3601 tccgctgcgc ggcaaaccca ggacaaagat cggccctgcc gcgtcgcaac ccttcgaggg
     3661 ttgggtaaac agcgccgcgt ggaaaacccg ctcgatttcc gccatggccg cgacgtccgc
     3721 ttcgacccga tagctgagca ggctgcgtcg acgttccgcg ccccgcctga gccaggcaaa
     3781 gctctcttcg tactcaccca ggtcctcgaa ctccttggcc agggcatagc tgatcgccac
     3841 ctcgccgggt ccggtcagac gcttccgcga cgcctggaga agcgccgccg tatggttgga
     3901 tccaggcgtc tgccgccgga gcgtcgagcg gttctgccac gcatcataat cctccggatc
     3961 aagctgaatg acgcggtcca ggagcgcttc ggcctcgccg gcctgaccca gggcgatcaa
     4021 ggccgtggcg agattgtaga gaccacgcgg gtcatccggc gcgagcgcag ccgcccgcct
     4081 gtaacagcgc gctgcatcgg ccgatctggc gcagtgggtg tagtgctctc ccaggtgctg
     4141 cagcaggcga gcgtcatcgc ccgcccgagc ctcaagagcg gccagcctgg caagggcgtc
     4201 tccaacgcga ccgtcgcgga tgagcggggc gacccggccc agctcatcca tcacgtcgtc
     4261 cctgatcgcc aggctaacca tcctgattct agaagctgta gtcgagcgac aggccgagcg
     4321 tccgcggcag cgagatgaac tgcttggacg cgttgccgta gtagccgacc gccgggcggg
     4381 tgcccatata ggcctcggtg aactcgccgg tcacgccgtc ctcgttgagg atgttcttga
     4441 cgaacaaggt cgccgcccag ggccctttgg ccaggg
//
LOCUS       DAOMEO010000105         4446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2396_length_4446_cov_2.641312, whole genome shotgun sequence.
ACCESSION   DAOMEO010000105 DAOMEO010000000
VERSION     DAOMEO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2396_length_4446_cov_2.641312"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..980
                     /locus_tag="PLH31_03475"
     CDS             108..980
                     /locus_tag="PLH31_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HQR88325.1"
                     /translation="MDAAALAGLCALLARGMSAQPLPAGVNCPAAMATSPSISAAAAP
                     PSGSSSLAARLGGPEARDAIARVAFAEAANQGDSGLAAVVYTIMNRLQDGRWGSSVEA
                     VLNARAQFEPVMRAGGSWRNLPPVSEAQRARGNTIINLAIEGRLPDLTNGARFFQNAR
                     IVAAREAAGAVSPGLTNFGGAPSSAVIGAHTFYVEAGRGDAAKGGGGRGGRSSSARPS
                     PTDSRSALSVPNDGIFVGENRAFSGPVTPPPEPVEGPAPVLTPASSGADFQAAPADQI
                     ADQPVDPTRASNGP"
     gene            1018..1449
                     /locus_tag="PLH31_03480"
     CDS             1018..1449
                     /locus_tag="PLH31_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88326.1"
                     /translation="MHKVGFAFLPSFVGRLRQKGAAEDDLAPGFALSLVDRPQCFEAF
                     DPGFQHGETLAVVRDIFERNGVALVVDSRAAHVTQGRLKLLQAKFQRFFIADPATQFA
                     IGFTGGPEVLALLLLGLAAGSIFGGPGVPLALIDRDHDRHP"
     gene            1694..3190
                     /gene="mobQ"
                     /locus_tag="PLH31_03485"
     CDS             1694..3190
                     /gene="mobQ"
                     /locus_tag="PLH31_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HQR88327.1"
                     /translation="MASFLCQVQTIQRGLGRSAVAAAAYRSGQALTDDRLAMEFDFSN
                     KDGVEHAEIMAPDDAPQAYRDRAALWNAVEAAERRKDAVPAQEVLLALPHELDFDQRR
                     DLVRAFVQEHLVAKGMIADVAMHRPGKEGDQRNFHAHIMVTTRRVDRGGFGGKNRDWN
                     HASFIPQLRAAWSDVQNRALRHHLGPDAPQVSHLSLAARGVDRAPTVHLGPAATALER
                     KDIASERGELNRDVRARNDKARDLRRDYQATADRIAKTAPEIEVAVPKLVVEAEQVLG
                     KMVAQRTAWAAEREALSAPKIPSAGRIERELTAEAATAKARARAHLQQTEERVKKTRS
                     RRLELVAWVRNPARMIWAKHAELNAIGRARKAYRRAEVGLQVRQDWVPSPKGQAFVAA
                     RREPGLEAAADVVRQRRTLERKIKRMDNRIGLAGRTINDLRLAHELGQRELRVPNQSP
                     DETRFFRDIGRPAREALHRFPTPVQEQALERLRRGQGRSIGRAIIPGR"
     gene            3205..>4446
                     /locus_tag="PLH31_03490"
     CDS             3205..>4446
                     /locus_tag="PLH31_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQR88328.1"
                     /translation="MNARPKIILPLVAASVLIALSIATQIVAHDFHYPREFGHGLLDV
                     GRLRIYAPWAFVGWYGRFAAHYQQAFDMAAMIALAAVFVPSMLLIGLTKSTRRAPREF
                     GKEAWATEAHVRKAKLVHGDGQISGRVLGRFNGKYLTYRGVEHAIIVGASRSGKGAGH
                     VVPTLIAWPQSAFVYDRKGELWHITADHRKTFSHVFYFAPTDPNTVRWNPLFEVRKGP
                     MEIADIQNVVGILVDPLGRKAGDLNFWDQSATDFFTAIILHVLYSEEDTKKNLAQVRR
                     LLINIDPTLHAMKHTKHRHRPDLHAPGGLARGKDGKPIAEVHPEILLGATALDSMDER
                     VKSNVLATCRASLSLWADPYVEYATSWSDFSIGDLVCSESPVTFYIITPQAHADRLAF
                     LVRVFTRQTINSLMEREHFDSR"
BASE COUNT          736 a         1518 c         1475 g          717 t
ORIGIN      
        1 atccccaaca gcttcgatag ccggtactac ggaccggttc gccgcgaagc gatcgtcggc
       61 gtcttccgcc cgatcataac ccagaccgac cgcccaggtg acgtctgatg gacgccgccg
      121 ccctcgcggg tttgtgcgcc cttctggccc gcggcatgag cgcccagccg ctgccggccg
      181 gcgtcaattg tccggccgcg atggccacgt cgccctccat atcggccgca gccgcacccc
      241 cgtcaggttc ctcgtcattg gccgctcgcc tgggaggacc agaggctcgc gacgccatcg
      301 cccgtgtcgc cttcgcggag gccgcgaacc agggcgacag cggcctggcg gccgtcgtct
      361 acaccatcat gaaccggctg caggatggcc gctggggctc aagcgtcgag gccgtgctga
      421 acgcccgcgc gcagttcgag ccagtgatgc gggccggcgg cagctggcgc aatctgccgc
      481 ccgtctcgga ggcccagcgg gctcggggca acaccatcat caacctggcg atcgagggtc
      541 gtttgccgga cctgacgaat ggcgcgcggt tcttccagaa tgcccggatc gtcgcggccc
      601 gggaggcggc cggcgcggtg tcgccgggcc tgacgaactt tggcggcgct ccatcctccg
      661 cggtgattgg cgcccacacc ttctatgtgg aagccggccg cggagacgct gccaaaggag
      721 gcgggggcag gggagggcgt tcgagctcgg ccaggccgtc gccgaccgac tccagatccg
      781 ctttgagcgt cccgaacgac ggcatcttcg taggcgagaa ccgggcgttt tccggacccg
      841 tcacgccgcc tccggagcct gtcgaaggcc ccgccccagt ccttacaccc gcctcgtcgg
      901 gggcggattt ccaggcggcg ccagcggacc agattgccga tcagccggtc gatcccaccc
      961 gggcgtcaaa cggtccctga cgctctccac ccggcggtcc aggatatcga ccagccgatg
     1021 cacaaggtcg gcttcgcctt cctgccaagc ttcgtcggcc gcctccgcca aaagggcgcc
     1081 gccgaggatg atcttgcccc gggtttcgcg ctttctctgg ttgatcgccc acagtgcttc
     1141 gaggcgttcg acccgggctt tcagcacggc gaaacgctgg ccgtcgtccg cgatatcttc
     1201 gagcggaacg gtgtggcgct tgttgtggat tcccgtgcgg cccatgtcac gcaaggccgc
     1261 ctcaagctcc tccaggcgaa gtttcagcgc ttcttcatcg ctgatccggc gacccagttt
     1321 gcgatcggct tcacgggcgg cccagaggtc cttgcgcttc tgcttctggg tctcgctgcg
     1381 gggtcgatat ttggagggcc gggtgttccg cttgcgctta tcgaccgcga tcatgacagg
     1441 cacccttgat gaagaaaaag agcgtaatgc actttttcta tcaaaatagc gcctgatggt
     1501 ccatggtggt ggcagaggtg ttgcagacaa tggcgcatat cctcattggt tgggctacgc
     1561 ccagccaacg atgcggtcag gctccgcccg acaccctcaa acccccgcac ggcctgcgac
     1621 ggcctggggt ttgagccaag ggccgagccc ttagcgatcc cgccggctca cgccggcagg
     1681 ggagggcagt tcagtggcgt cgttcctgtg tcaggtgcag accatccagc gcggtcttgg
     1741 ccgctcggcc gtggccgcag cggcgtaccg gtcaggccag gcgctgaccg atgaccggct
     1801 ggccatggag ttcgacttct cgaacaagga cggggtcgag cacgccgaga tcatggcgcc
     1861 ggatgacgcg ccgcaggcct acagggaccg cgcagccctg tggaacgccg tcgaggccgc
     1921 ggagcgccgt aaggacgccg tgcccgccca ggaggtcctc ctggcgctgc cgcacgaact
     1981 tgatttcgac cagcgccggg acctggtccg cgcctttgtg caggagcacc tggtcgccaa
     2041 gggcatgatc gctgacgtcg ccatgcaccg gcccggcaag gagggcgacc agcgcaattt
     2101 ccacgcccac atcatggtca cgacccgccg tgttgatcgc ggcggtttcg gaggcaagaa
     2161 ccgcgattgg aaccacgcca gcttcatccc gcagctgcgc gccgcgtggt cggacgtgca
     2221 gaaccgggcc ctgcgtcatc accttggtcc ggacgccccg caggtctcgc atttgagtct
     2281 ggcggcgcgc ggcgtcgacc gcgccccgac tgtgcatctg gggccggcgg ccacggcgct
     2341 ggagcgcaaa gatatcgctt ctgagcgtgg cgaattgaac cgcgatgtcc gggcccgcaa
     2401 cgacaaggcg cgcgaccttc gacgcgacta tcaggcgacc gccgaccgca tcgccaagac
     2461 cgctcctgag atcgaagtcg cggtcccgaa gctggtcgtc gaagccgagc aggttctagg
     2521 caagatggtc gcccagagga cggcgtgggc ggccgaacgc gaggcgcttt ccgcgccgaa
     2581 aatcccgagc gccggccgga tcgaacgcga gctgacggcg gaggcagcga ccgccaaggc
     2641 gcgggcgcgg gctcaccttc agcagaccga ggagcgcgtg aagaagaccc gttcgcggcg
     2701 cctggaactg gtggcctggg tccgcaatcc ggcgcggatg atctgggcga aacacgccga
     2761 gctgaacgcc atcggacgcg cccgaaaggc ctatcgccgg gcagaggttg gcttgcaagt
     2821 ccgccaggat tgggtcccct caccgaaagg ccaggcgttc gtcgcggcgc gccgggagcc
     2881 gggcctggaa gccgccgccg acgtggttcg gcaacggcgc acgctggaac ggaagatcaa
     2941 gcggatggac aaccggatcg ggttggccgg ccgcacgatc aacgacctgc ggctggcgca
     3001 cgaacttggc cagcgggagc ttcgtgtccc aaaccagtcg cccgacgaga cccgcttctt
     3061 tcgggatatc ggccgcccgg cgcgcgaggc cctgcatcgt ttcccgacgc cggtccagga
     3121 acaggcgctg gagcgtctgc gacgtggcca gggccggtcg attggccgcg ccatcattcc
     3181 cggccggtag gagcgacacg ccccatgaac gcccgcccca agatcatcct gccgctggtg
     3241 gcggccagcg tgctgatcgc cctgtcgatc gccacccaga tcgtcgctca cgacttccac
     3301 tacccgcgtg agttcggcca cggcctattg gatgtggggc ggctaaggat ctacgcgccg
     3361 tgggcgttcg tcggctggta tgggcgcttc gcggcgcact accagcaggc cttcgacatg
     3421 gcagcgatga tcgcgctcgc ggccgtattc gtcccctcga tgctgctgat cgggctgacc
     3481 aagagtaccc gccgggcgcc gcgggagttt ggcaaggagg cctgggcgac cgaggcccat
     3541 gtccgcaagg ccaaactcgt tcatggcgac ggccagatca gcggacgggt gctcggccgg
     3601 ttcaacggca aatacctcac ctatcgcggt gtggagcacg ccattatcgt gggcgcgtcc
     3661 cgcagcggga agggggccgg ccacgtcgtt cccaccctga tcgcctggcc gcaaagcgcc
     3721 ttcgtctacg accgcaaggg cgagctgtgg cacatcacgg ccgatcaccg gaagacgttc
     3781 agccacgtct tctatttcgc gccgaccgac cccaacaccg tgcggtggaa tccgctgttc
     3841 gaggtgcgga aggggccgat ggagatcgcc gacatccaga acgtcgtcgg catcctggtg
     3901 gacccgctgg gccgcaaggc cggcgacctc aatttctggg accagagcgc gacggacttc
     3961 ttcactgcga tcatcctgca cgtcctctac agcgaggagg acaccaagaa gaacttggcc
     4021 caggtccgcc gcctgctgat caatatcgac ccgacccttc atgcgatgaa gcacaccaaa
     4081 catcgccata ggccggacct tcatgcgccg ggcgggctgg cgcggggcaa ggacggcaag
     4141 cccatcgccg aggtccaccc ggagatcctg ctaggcgcca cggcgctgga cagcatggac
     4201 gagcgggtga agtctaatgt gctggccacc tgccgcgcgt cgttgtcgct gtgggccgac
     4261 ccgtatgtgg aatacgccac cagctggtcg gacttctcga tcggcgacct ggtgtgctcg
     4321 gagagcccgg tcaccttcta catcatcacc ccccaggccc atgccgaccg gctggccttc
     4381 ctggtgcggg tgttcacccg ccagacgatc aacagcctga tggagcgcga gcatttcgac
     4441 agccgg
//
LOCUS       DAOMEO010000106         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2410_length_4417_cov_2.684778, whole genome shotgun sequence.
ACCESSION   DAOMEO010000106 DAOMEO010000000
VERSION     DAOMEO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_4417_cov_2.684778"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..>857
                     /locus_tag="PLH31_03495"
     CDS             84..>857
                     /locus_tag="PLH31_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQR88329.1"
                     /translation="MWPALSALALAAVLATAVALMIPVSARAAQPAAITATAAPGAFP
                     LIRAGQPARVEADVTDHPGALRAITDLRTDLARLSGAPAPTKDAPVVIIGTIGKSAAI
                     DALIASGKLDVSKVTGQWDGFVQQVIDNPRPGLKQALVIAGADKRGTIYGAYDLAERA
                     GVSPWTWWADAPVIVRKDVFVAPGANIQKPRVKYRGIFLNDENPALYGWANKTFGGFN
                     HAFYERVYELTLRLKGNYLWPAMWGKALYDDDPLNAPLAD"
     assembly_gap    858..867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <868..>1434
                     /locus_tag="PLH31_03500"
     CDS             <868..>1434
                     /locus_tag="PLH31_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQR88330.1"
                     /translation="VIGTSHHEPMMRAHIEWERHGSGRWDYAKNPDKLREFWREGVER
                     MGGNESLVTVGMRGDGDEPMSEGTATQLLETIVADQRKIIGEVTGKPPEKTPQVWALY
                     KEVQDYYDAGMRVPDDVTLLFADDNWGNIRRLPEPGKTRLGGYGVYYHFDYVGGPRNY
                     KWLNTNQIERVWEQMKLADDYGADRLWIV"
     assembly_gap    1435..1444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1445..2923
                     /locus_tag="PLH31_03505"
     CDS             <1445..2923
                     /locus_tag="PLH31_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQR88331.1"
                     /translation="DLKPMELPTEFFLDYAWNPDAIPLETLSTYTRDWAAAQFGPEQA
                     TQIAALLDGYTKLNARRKPELIDPSTFSLIHDREAERVDAEWAELERRADAVRAALPK
                     TADDAFFQLVWFPVKASANLTRLYIAAGRNRLYAAQGRIGGAYAQADRVEALFKRDAE
                     LTRQHDAIAGGKWVHMMDQTHIGYTSWQQPDRNIMPAVVRPSPPRLPLARIGVAIEGR
                     EAAVTGAAELPVLHRYGAPSRWIDVFDTGFSTATFEVSTGAPWLKVVRGAPDPHGDAR
                     LEVSVDWARAPKGLARAPITIKGVTNIFTITAVISNPARQPAKGAFVEAGGVVAIEAE
                     HHARATSADGVGWKTIPNLGRTLSGVVAYPTTAASSVPGKGAALEYLIDFEKAGPTDL
                     TVFVSPSLDFRGNRGLRYAVSINDAPPVTVNIIPDPSERAWDKAVADNIRRLTTRLEI
                     PSAGAHRVRLWRVDPGVVFQRLVLSRAPPSGSYLGPVESVRR"
     gene            complement(3057..3806)
                     /locus_tag="PLH31_03510"
     CDS             complement(3057..3806)
                     /locus_tag="PLH31_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQR88332.1"
                     /translation="MADNVKLYRRIADTVAEAIEAGQYKLGDRLPTERELAEQFGVSR
                     PTLREAMIALEMLGMIEARHGLGIYVTGNVRPSAPAAEIDFEIGAFELIEARRLFEGE
                     AAALAATSIDDAQLAELEALMVRMHEEEEIAGEDADREFHMIIARATGNGAIIATIEN
                     LWDWRNRSPLARNILARARGMGLEPRIHEHRRVFEALKARDPHAARQAMRDHLERVIE
                     HLLHATETEAVQRAQQETSARRSAIAKRVAI"
     gene            complement(3930..>4417)
                     /locus_tag="PLH31_03515"
     CDS             complement(3930..>4417)
                     /locus_tag="PLH31_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HQR88333.1"
                     /translation="ENQDNFRLVRQHTTTPLAVGEVFNSIWDCKQLIQEQLIDYIRAT
                     VVHAGGITHLRRIASFADMCNVRTGCHGATDLSPVSMAAALHFDLSIPNFGVQEYMRH
                     TPETDAVFPHAYSFSDGMLHPGEAPGLGVDIDETLAAGHPYKRAYLPIARREDGSMHD
                     W"
BASE COUNT          703 a         1496 c         1499 g          699 t
ORIGIN      
        1 gtgcagccgt gaccctaacc tccgctctcc ccggcgacac gtcagtgcgg cgaagccaac
       61 gccggggaga gcgggatagg gggatgtggc cggcgctgtc ggccttggcg ctcgccgccg
      121 ttctggcgac cgctgtcgcc ttgatgatcc cggtctcagc acgagccgcc caacccgccg
      181 cgatcaccgc caccgccgcc cccggcgcct tccccctgat ccgcgccggc cagcccgccc
      241 gcgtcgaggc ggacgttacc gaccaccccg gcgcgcttcg tgcgatcacc gacctcagga
      301 ccgacctcgc caggctctcg ggcgcccccg cgcccacgaa agacgccccg gtcgtcatca
      361 tcggcacgat cggcaagagc gccgccatcg atgccctgat tgcgtccggc aagctggacg
      421 tctccaaagt gaccggccag tgggacggtt tcgtccagca ggtgatcgac aatccccgcc
      481 ccggcctcaa gcaggccctg gtcatcgccg gcgccgacaa gcgcggcacg atctatggcg
      541 cctatgacct cgccgagcgg gccggcgtct caccgtggac ctggtgggcc gatgcgccgg
      601 tgatcgtgcg caaggacgtc ttcgtcgccc ccggcgccaa catccagaag cccagggtca
      661 agtaccgggg catcttcctc aatgacgaga acccggccct gtacggctgg gccaacaaga
      721 cctttggcgg cttcaaccac gccttctacg agcgggtcta tgagctgacc ctgcggctga
      781 agggcaacta tctgtggccg gccatgtggg gcaaggcgct gtatgacgac gacccgctga
      841 acgccccgct ggccgacnnn nnnnnnnggt gatcggcacc tcgcaccacg agccgatgat
      901 gcgggcccac atcgagtggg agcgccatgg ctccggccgt tgggactatg cgaagaaccc
      961 cgacaagctg cgcgagttct ggcgcgaggg cgtcgagcgc atgggcggca atgagagcct
     1021 ggtcaccgtc ggcatgcgcg gcgacggcga cgagccgatg agcgagggca cggccaccca
     1081 gctgctggaa accatcgtcg ccgaccagcg caagatcatc ggcgaggtga ccggcaagcc
     1141 gcctgagaag acccctcagg tctgggcgct ctacaaggaa gtgcaggact attacgacgc
     1201 cggcatgcgc gtgcccgacg acgtgaccct gctgttcgcc gacgacaact ggggcaacat
     1261 ccgccgcctg ccggaacccg gcaagacgcg gctgggcggc tacggcgtct attaccactt
     1321 cgactatgtg ggcgggcccc ggaactacaa gtggctcaac accaaccaga tcgagcgcgt
     1381 ctgggagcag atgaagctgg ccgacgacta cggcgccgac cgcctgtgga tcgtnnnnnn
     1441 nnnngacctg aagcccatgg aactgcccac cgagttcttc ctcgactacg cctggaaccc
     1501 tgacgccatc ccgctggaga cgctgtcgac ctacacccgc gactgggcgg cggcgcagtt
     1561 tggcccggag caggcgaccc agatcgcggc cctgctggac ggctatacga agctcaatgc
     1621 gcggcgaaag cccgaactga tcgacccgtc gaccttcagc ctgatccacg atcgcgaggc
     1681 cgagcgggtg gacgccgaat gggccgagct ggagcgccgc gccgacgcgg tgcgcgcggc
     1741 tctgcccaag accgccgacg acgccttctt ccagctggtc tggtttccgg tgaaggccag
     1801 cgccaacctg acccgtctct acatcgccgc cggtcgcaac cggctgtatg cggcgcaggg
     1861 acgtattgga ggagcctacg cccaggccga ccgggttgag gccctgttca agcgggacgc
     1921 cgaactcacc cgccagcacg acgccatcgc cggcggcaag tgggtccaca tgatggatca
     1981 gacccacatt ggctacacca gctggcagca gcccgaccgc aacatcatgc ccgccgtcgt
     2041 tcgacccagt ccgccgcgcc tgccgctggc caggatcggc gtggcgattg aagggcgcga
     2101 ggcggcggtg acgggagcgg cagagttgcc tgttctgcac cgctacggcg cgccctcgcg
     2161 ctggatcgac gtcttcgata cgggcttctc gacggcgact ttcgaggtct cgacaggcgc
     2221 gccctggctg aaggtcgtgc gcggcgcgcc cgatccgcat ggcgacgcgc ggctagaagt
     2281 ctcggtggac tgggcccggg ccccgaaggg gctggcccgc gcgccgatca ccatcaaggg
     2341 cgtgacgaac atcttcacga tcaccgccgt gatctccaac cccgcccgcc agccggccaa
     2401 gggcgccttc gtcgaagccg gcggcgtcgt cgccatcgaa gccgagcacc acgcccgcgc
     2461 gacgtcggct gacggcgtcg gctggaagac catcccgaac ctgggccgca ccctgtcggg
     2521 cgtcgtggcc tatcccacga cggcggcgtc cagcgtcccc ggcaaggggg ctgcgctgga
     2581 gtatctgatc gatttcgaga aggccgggcc caccgacctg acggtcttcg tttcgcccag
     2641 cctggacttc cggggcaata ggggcctgcg ctacgccgtc tcgatcaatg atgcgccgcc
     2701 ggtcactgtg aacatcatcc ccgacccgtc cgaacgcgcc tgggacaagg cggtagccga
     2761 taatatccgt aggctgacga ctcggcttga gattccctcg gccggcgctc accgcgtcag
     2821 gctatggcgc gtggatccgg gcgtggtgtt ccagcgtctg gtgctgtcgc gcgccccgcc
     2881 aagcggcagc tatctggggc cagtggagag cgtccggcgg taggatgtca gcgcgccctt
     2941 ctgggtcgcc tccagccgcg cgtggcggcg aatctccgca caagcaagag aatatccgca
     3001 ttatatgcgg agaatggagg ctctattctc cgcaggatgg cgcgggaggc aaagccctag
     3061 atcgccaccc gcttggcgat cgcgctgcgg cgggcgctgg tttcctgctg ggcgcgctgg
     3121 acggcttcgg tctcggtcgc atgcagcagg tgttcgatca cccgctccag atggtcgcgc
     3181 atcgcctgcc gcgcggcgtg agggtcgcgg gccttgagcg cctcgaacac ccggcggtgc
     3241 tcgtggatgc ggggctccag ccccatgccg cgggcgcggg ccaggatgtt gcgggccagg
     3301 ggcgagcggt tgcgccagtc ccacaggttc tcgatggtgg cgatgatcgc gccgttgccg
     3361 gtggcccggg cgatgatcat gtggaactcg cggtcggcgt cctcgccggc gatttcctct
     3421 tcctcgtgca tgcgcaccat cagggcctcc agctcggcga gctgggcgtc gtcgatgctg
     3481 gtggcggcca gggcggcggc ctcgccctcg aacaggcggc gggcctcgat cagctcgaag
     3541 gccccgatct cgaaatcgat ctcggcggcg ggggctgagg gacgcacatt gccggtaaca
     3601 tagatgccaa gcccgtggcg ggcctcgatc atgcccagca tttccagggc gatcatggcc
     3661 tcgcgaaggg tggggcggct gaccccgaac tgttcggcca gctcgcgctc ggtcggcagg
     3721 cggtcgccca gcttgtactg cccggcctcg atggcttcgg ccacggtgtc ggcgatacgg
     3781 cggtagagct tgacgttatc ggccatggac gggtgcttgg gtctcggaac ggcgcggggg
     3841 ccgggtcaga tcggcttcac ctcaagtatc acgccgattc cagaccttca cacgcctttt
     3901 caccgatggc gcgccgtttt gcctgtcgat caccaatcgt gcatcgagcc gtcttcgcgg
     3961 cgggcgatcg gcaggtaggc gcgcttgtag ggatggccgg ccgccagggt ctcgtcgatg
     4021 tcgacgccca ggcccggggc ctcgcccgga tgcagcatgc cgtcgctgaa gctgtaggcg
     4081 tgggggaaca ccgcgtcggt ctcgggcgtg tggcgcatat attcctggac gccgaaattg
     4141 gggatcgaca ggtcaaagtg cagggccgcg gccatgctga ctggcgacag gtcggtggcc
     4201 ccgtggcagc cggtgcggac gttgcacatg tcggcgaagc tggcgatcct gcgcaggtgg
     4261 gtgatgccgc cggcatgcac cacggtggcg cggatatagt cgatcagctg ctcctggatc
     4321 agctgcttgc agtcccagat cgaattgaac acctcgccca cggccagggg ggtggtggtg
     4381 tgctggcgga ctaggcggaa attgtcctgg ttctcgg
//
LOCUS       DAOMEO010000107         4405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2421_length_4405_cov_2.085517, whole genome shotgun sequence.
ACCESSION   DAOMEO010000107 DAOMEO010000000
VERSION     DAOMEO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_4405_cov_2.085517"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1722)
                     /gene="glpK"
                     /locus_tag="PLH31_03520"
     CDS             complement(229..1722)
                     /gene="glpK"
                     /locus_tag="PLH31_03520"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQR88334.1"
                     /translation="MAEQLILALDQGTTSTRAILFDTTGGALADATRPLRQSYPADGW
                     VEHDAEEIFAACVAVLREAMEASGRDVGDVAAIGITNQRETVVIWDKATGRPIHPAIV
                     WQDRRTADACERLREAGHEAQVTEVTGLLLDPYFSGTKIAWILDRVQGARARAEAGEL
                     LCGTMDSWVIWRLTGGAVHATDATNASRTLLFDIEVQDWSQPMLELLGVPVTLLPKVL
                     DCADDYGVTTADILGREVPIRGVAGDQQAALMGQGCVQPGQMKATYGTGCFMLLNTGE
                     KRPVSRSRLLTTVAARVGGKTTYALEGSIFVAGAAIQWLGEGLGITGGPRSAEALARS
                     AKPDHGVVVVPAFTGLGAPWWDAGARGAIFGLTRDAGLPEIAAATYDSCALQSRDLIE
                     AMRADAPHALGGDAELRIDGGMSKSAWFSQRLADLTGMLVQRASYLETTALGAALFAG
                     VGAGVYASLETAAAARPAAEALTPSLPLHQREAAYARWLDAVPRVRT"
     gene            1892..2947
                     /locus_tag="PLH31_03525"
     CDS             1892..2947
                     /locus_tag="PLH31_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HQR88335.1"
                     /translation="MTLARSRDPGDRERLLTGIIELCEAGQTAGAPTDPDIQRLLDSI
                     FMTLVVEAERDIRQRLSERLADAPWAPSALINILALDEIEIARPIIARSPVLQDHDLI
                     RLMVQATLEHQIEVARRPRLPASVVEAILAREEPAVLTALASNDTAEISTDGLRRLVG
                     RARDVVALRSPLARHPMLSSELAEQLYLWVGRALREALTARFHLDADVMANAVDEALR
                     AAHRGDDAGQDGVIESEEGREEMERSLVEKLDSAGQLRPGYLLRVLREGRLPLFILAL
                     ARLGKFDSAQIRRAIDSNRPELLALACSAILEHVRQLNGGRPGGGAEGARRAGSAFGP
                     FTPDVAGMAFRQAVGAV"
     gene            2980..3762
                     /locus_tag="PLH31_03530"
     CDS             2980..3762
                     /locus_tag="PLH31_03530"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQR88336.1"
                     /translation="MYQPQPFLTRLTFKASDRPEAEDARERLIARYGDVGEESAQVIV
                     ALGGDGFMLETLHGAIASQTPIYGMNRGSVGFLMNEYSEDGLLERINAAERAVIHPLV
                     MTAIDSQRRNHRALAINEVSLLRQTRQTAKLRISIDGKVRMSELVCDGALIATPAGST
                     AYNLSAHGPIIPIGGQVLALTPISAFRPRRWRGALLPRTARITFEIIEADKRPVSAVA
                     DNFEVRDVMEVHISEDRDTSLAMLFDAGRSLDERVLAEQFSA"
     gene            3855..4337
                     /locus_tag="PLH31_03535"
     CDS             3855..4337
                     /locus_tag="PLH31_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQR88337.1"
                     /translation="MTTKPTAEVIQVPNMLKLKVGGRGGIDMAAIAKAEAALKSLSGN
                     FAQWLQDEITKLEAARQEVKTQGLTATTVETLYLRAHDLKGLGATYEFPLITRIAASL
                     CKLIDEPETRLSAPMFLVDAHIDAIKAAVRGDIKVDTHPVGKALAEELEGRVTEYLKG
                     "
BASE COUNT          752 a         1514 c         1401 g          738 t
ORIGIN      
        1 acgcgaccac cgcgtccagc gccggagaca ggcggcggat gtttcccacg gtcgggcgag
       61 cggcatgggc agacatggag aaccctcctg cgagaacggc ggcgcttgcc cccaaggtga
      121 tggaacggcg cgtcggcgtc atgcggttgg gtcctcatgt tatgacagcg atgtcatagc
      181 aactgcgcgt atggctggcg acccgcgttc tgctttcgcc gacagaaatc aggttcggac
      241 gcgcggtacg gcgtccagcc accgcgcata ggccgcctcg cgctgatgca acggcaggga
      301 cggcgtaagc gcctcggccg cgggccgcgc ggcggccgcg gtttcaagcg aggcgtaaac
      361 gccagcgccg acgccagcga acagggcggc gcccagggcc gtcgtctcca gatagctggc
      421 ccgctgcacg agcatccccg tgagatcggc gagccgctga gaaaaccagg cgcttttcga
      481 catgccgcca tcgatacgca gttctgcatc gccccccagg gcgtggggcg catcggcccg
      541 catggcttcg ataaggtccc ggctctgcag ggcgcaggag tcataggtcg ccgcagcgat
      601 ctccggcagg ccggcgtcgc gggtcaggcc aaagatcgcg ccccgcgctc cggcgtccca
      661 ccagggcgcg ccaaggcctg tgaaggccgg caccaccacg acgccatggt cgggcttggc
      721 cgaccgcgcc agggcttcgg ccgagcgagg gccaccggta atcccaagcc cctcgcccag
      781 ccactggatc gccgcgcccg ccacgaagat cgagccctcc agggcgtagg ttgtcttgcc
      841 gccaacccgg gccgcgaccg tggtcagcag acgcgagcgc gacaccgggc gcttctcgcc
      901 cgtattgagc aacatgaagc agccggtgcc gtaggtggcc ttcatctggc cgggctggac
      961 gcagccctgc cccatcagcg ccgcctgctg atcacccgcg acgccccgga ttggcacctc
     1021 acggcccagg atgtcggctg ttgtcacgcc atagtcatcc gcgcagtcga gcacctttgg
     1081 caggagcgtc acgggcaccc ccagaagctc caacatgggc tgcgaccagt cctggacctc
     1141 gatgtcgaac aggagggtgc gtgaagcatt ggtggcgtcg gtggcgtgca ccgcgccgcc
     1201 cgtcaaacgc cagatcaccc agctatccat ggtgccgcac aacagttcgc cagcctcagc
     1261 gcgcgcccgg gcgccctgca cgcgatcgag aatccaggcg atcttcgtcc cggaaaaata
     1321 gggatccagc agcaggcccg tcacctctgt aacctgggct tcatggccag cctcgcgaag
     1381 ccgctcgcag gcgtcagcgg tacggcggtc ctgccagacg atagcgggat ggattggccg
     1441 ccccgtcgcc ttgtcccaga tcaccaccgt ctcgcgctgg ttggtgatgc caatggcggc
     1501 cacatcgccg acgtcacggc ccgacgcctc catcgcttca cgcagcacag ccacacaggc
     1561 ggcgaagatt tcctcggcgt cgtgctcgac ccatccgtcg gccggatagc tctgcctcag
     1621 gggtcgcgtg gcgtcggcca gcgcgccgcc ggtggtgtcg aaaaggatcg ctcgtgtgct
     1681 ggtggtgccc tgatccaggg ccagaatcag ttgttcagcc atactcgcgc ctcccgtcct
     1741 tacgcttcac ccgcgacaac gcgccgctgt tcttatcgtt tgttgggttg ctccgtttaa
     1801 gcatgttttc atttgccgga agcaaggtca tcctcaccga gttccggatt cgaggaggct
     1861 ggtcatccaa gccgtccaaa ccgccaagct tctgacactg gcgcgcagcc gcgatccggg
     1921 cgatcgtgaa cggctgctca ccggcattat cgaactgtgc gaagccggcc agaccgccgg
     1981 cgccccgacc gatcccgaca ttcagcggct actcgactcc atcttcatga cactggtcgt
     2041 ggaagccgaa cgtgatattc gccagcgcct gtccgaacgc ctggccgacg ccccttgggc
     2101 gccctccgcg ctaatcaaca ttctggcgct cgacgagatc gagatcgccc gcccgatcat
     2161 cgcgcgaagc ccggtgctgc aggatcatga tctcatccgc ctgatggttc aggcgacgct
     2221 ggaacatcag atcgaagtgg ctcgccggcc gcgcctgcca gcttcggtcg ttgaggccat
     2281 cctggcgcgt gaagaacccg ccgtgctgac ggccctggcc agcaacgaca ccgccgagat
     2341 ctcgaccgac ggactgcgcc ggctggtcgg tcgcgcccgg gacgtggttg ccctgcgctc
     2401 gccattggcg cgccacccga tgctctccag cgaattggcc gagcagcttt atctgtgggt
     2461 cggccgcgcc ctgcgcgaag cgctcaccgc ccgcttccat ctggatgcgg acgtgatggc
     2521 caacgccgtc gatgaagcgc ttcgcgccgc ccatcgcggt gacgatgcag gccaggacgg
     2581 tgtcatcgag tcggaagaag gccgcgagga gatggagcgc tcgcttgtcg agaagctcga
     2641 ctccgccggg caacttcggc ccggctatct gctgcgggtg ctccgcgaag gccgcctgcc
     2701 cctgttcatc ttggccctgg cgcgtctggg caagtttgat tcggcccaga tccggcgagc
     2761 catcgacagc aaccgccccg aacttctggc cctggcctgc tcggccatcc tggagcatgt
     2821 gcgtcaactg aatggcggcc gcccgggcgg cggcgcagag ggggccaggc gcgctggcag
     2881 cgccttcggg cccttcacgc cggacgtcgc cgggatggct ttccgacagg ctgtcggcgc
     2941 ggtttgacaa gccggcgggc ttcccgcctc ttgggcgcga tgtaccagcc tcagcctttc
     3001 ctgactcgcc tgacgttcaa ggccagcgac cgccccgaag ccgaagacgc ccgcgagcgt
     3061 ctgatcgccc gctatggcga tgtgggggag gagtctgctc aggtgattgt cgcgctgggc
     3121 ggcgacggct tcatgctcga gaccctgcat ggcgccatcg ccagccagac cccgatctac
     3181 ggcatgaacc gcggatcggt tggcttcctg atgaacgagt acagcgaaga tggtctgctg
     3241 gaacggatca acgccgccga gcgcgccgtg atccatccgc tggttatgac cgccatcgac
     3301 tcgcagcgcc gcaaccatcg cgccctggcc atcaacgagg tctccctgct gcgccagacc
     3361 cgccagaccg ccaagctgcg catctcgatc gatggcaagg tgcggatgag cgagctggtc
     3421 tgcgacggcg ctctgatcgc aaccccggcc ggctcgacgg cctataacct gtcagcgcac
     3481 gggccgatta ttccgatcgg cggccaggtt ctggctctga cccccatcag cgccttccgc
     3541 ccccgccgct ggcgcggggc cctgctcccg cgcaccgccc gcatcacctt cgaaatcatc
     3601 gaggccgaca agcgcccggt cagcgctgtt gctgacaact tcgaggtgcg cgatgtgatg
     3661 gaggtccaca tctcggagga tcgcgacaca agtctggcga tgctgttcga tgcgggccgc
     3721 agtcttgatg aacgcgtgct cgctgagcaa ttttcggcct gaggcgttcc gccgcataga
     3781 aacgcacatc tcattcgttg ttaacgcctg ctatgatagc ggtcttggaa atacccgccg
     3841 ctggggaggc gatcttgacg actaagccca ccgccgaagt gatccaggtg ccgaacatgc
     3901 tgaagctcaa ggtgggcgga cgcggcggca tcgacatggc cgcgatcgcc aaggccgaag
     3961 ctgcgctgaa gagcctctcg ggcaacttcg cccagtggct gcaggacgag atcaccaaac
     4021 tcgaagccgc ccgccaggag gtcaagacgc agggcctgac cgcgacgacg gtcgagaccc
     4081 tgtatctccg cgcccacgat ctgaagggcc tgggcgcaac ctacgaattc ccgctgatca
     4141 cccgtatcgc cgcgtcgctg tgcaagctga tcgacgagcc cgagacgcgc ctgtcagcgc
     4201 ccatgttcct ggtggacgcc catatcgacg ccatcaaggc ggcggtacgc ggcgacatca
     4261 aggtcgacac ccaccctgtg ggcaaggccc tggccgaaga actcgaaggc cgcgtgacgg
     4321 aatacctgaa gggctgatcc ttcagagcag gtgatctttg ttgtctcagc tgtttatgtt
     4381 tatttcaggg gcaaggtgcg atttg
//
LOCUS       DAOMEO010000108         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2423_length_4402_cov_2.445595, whole genome shotgun sequence.
ACCESSION   DAOMEO010000108 DAOMEO010000000
VERSION     DAOMEO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_4402_cov_2.445595"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..960
                     /gene="rlmN"
                     /locus_tag="PLH31_03540"
                     /pseudo
     CDS             1..960
                     /gene="rlmN"
                     /locus_tag="PLH31_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
     gene            1065..1493
                     /locus_tag="PLH31_03545"
     CDS             1065..1493
                     /locus_tag="PLH31_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HQR88338.1"
                     /translation="MPSQLQSPEVQAFATGFPVTLMHAGVTLFMLIVGTALYALLTPH
                     REIKLIREGNSAAALSLGGVMVGLAIPLAISLTASTSVIEIVIWGAATIAVQLLVFRI
                     TDMVLQGLPERIHEGEVAAAALLVGAKLATALVLAAAVAG"
     gene            1519..2349
                     /locus_tag="PLH31_03550"
     CDS             1519..2349
                     /locus_tag="PLH31_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HQR88339.1"
                     /translation="MHFPRLPDWLVYAAAVSALLIVAVGRQERSDAPPPPPPVPGEEG
                     LPLGPASPFDPSIVVQVPNTPEPGSGTAFSVGDDGTWLTARHVVDGCKSAAIVVADGR
                     GVAARITIDPRADAAILTTEGGAPAVPLGLEEPLRRGARAYHPGFPQGHPGEAASRLL
                     GRENLVVHGRGARTEPVLVWAEVGRTDTLKGTLAGLSGAPALDSAGRVIGVTVAESPR
                     RGRIYTTAPETLKTLLGPRRRSGADIAMGEAITTENYGRVADGLRRDLRVAQVVCLGV
                     "
     gene            complement(2383..4095)
                     /locus_tag="PLH31_03555"
     CDS             complement(2383..4095)
                     /locus_tag="PLH31_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88340.1"
                     /translation="MTDQPAAKPQKKTLLQTLAFFGERRSLAMLGLGFASGLPFMLIF
                     DTLSLWLRDSGVSLAVIGFFSLATLSFSFKFLWAPLIDRARVPVLHKLMGHRRAWILV
                     FQIAMILGLWTISTLNPASQLGILAAVAVAVGFATASHDVVLDAWRIEVADKEEQGPM
                     AVAYQWGYRGAMIAAGAGPLLLAERFGWNFSYAVMGFVVGIGVMATLWAPREAEHTVR
                     PIPTGDVNQSRPLEILEWIVRLGILLVGALALGSGLSGDASLLSKLVGGEALAEAWKA
                     KPNGVWLQLLGVLTGLAIVLVAAWPIPKVRTKPGVYMSAAFGDPLIDFTRRFHGVAGL
                     ILAAICVYRVSDFVLNIMNPFYRDMGFTLTEIAEIRKVFGIIASMIGVFLGGVIVARL
                     GLMKALVIGAFAQPISNLFFAWLAMSGHNVPVLFWAICIDNVAGGVAGTALIAYMSSL
                     TTVGFTATQYALFSSLYALPGKLIASQSGRIVEASAIAAEAGGPIGALKGLFTRLPPE
                     SFTAAGAKLGVSAPAMAAGYTAFFLYTALIGVAAIVLSFMVAARQGEIDRRAEAEEAA
                     AKPA"
     gene            complement(4128..>4402)
                     /locus_tag="PLH31_03560"
     CDS             complement(4128..>4402)
                     /locus_tag="PLH31_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88341.1"
                     /translation="DLILMDLRMPVLNGIDATRRLRAQGVATPIVALTADAFDEDRRA
                     CLAAGMTDFLVKPLTHAALRDVLTRWTGAGAPDVWTKTASRAKVAG"
BASE COUNT          742 a         1529 c         1464 g          667 t
ORIGIN      
        1 ggcaaggcca agatgcgggc cacccaggta ttccgctgga tccaccatcg cggcgtcacc
       61 gatttttcca tgatgaccga cgtcgccaag gaaacccgcg cgcgcctggc cgaggtgttc
      121 accatcgcgc gcccggaaat cattgaacgt caggtctcca aggacggcac gcgcaagtgg
      181 ctgatccgca tggctcccgg catcgaggtc gaggcggtct atatcccgtc ggtcggccgg
      241 gccggcgcct tgtgtgtgtc cagccaggtg ggctgcacgc tcaattgcag cttctgccat
      301 accggcaccc aggccctggt ccgcaacctg accgccgccg agatcgtcgc tcaggtgcag
      361 gtagccaagg acgacctggc cgaatggccg tccgacaagg acgaccgcct gctgtccaac
      421 atcgtgttca tgggcatggg cgagccgctc tacaacatgg gctcggtcgc cgacgccatc
      481 gagatcatca gcgacaatga gggcatcgcc atctcgcgtc gccgcatcac ggtctcgacc
      541 tcgggtgtcg tgccccagct gcaggccctg ggcgagaaga cgcaggctat gctggccatc
      601 agcctgcacg ccaccaacga tcccctgcgc gacgtgctgg tgccgctgaa caagaagtat
      661 ccgctggatc agctgatcgc cggcatccgc gcctatccgg gcatcagcaa cgcccgccgg
      721 gtgacgttcg aatacgtgat gctcaagggc gtcaatgaca gccccgatga ggcccgcgcc
      781 ctggtcaagc tgatcaaggg cattccggcc aaggtcaatc tgatcccgtt caatccctgg
      841 ccgggctcgg actatcagtg ctcagactgg gccgcgatag aggccttcgc cgccatcctc
      901 aacaaggctg gctattcctc gccgatccgc acgcccagag gccgcgacat ccttgcggct
      961 tgaggtcagg gtaaggcgga acacgttccg agcgagcccc ctacgcaacc ctcgccgacc
     1021 cgtgctagtc cttgatcctc caggttccga gggtacccgc gttcatgccg tcccagcttc
     1081 agtcgccaga agtccaggcc ttcgccacgg gctttcccgt caccttgatg cacgcgggcg
     1141 tgaccctgtt catgctgatc gtcggcaccg cgctctacgc gctgctgacc ccgcaccggg
     1201 aaatcaagct gatccgcgag ggtaactccg ccgccgccct gtcgctgggc ggggtgatgg
     1261 tgggcttggc cattccgctg gccatttcgc tgaccgcctc gacctcggta atcgagatcg
     1321 tgatctgggg cgcggccacc atcgccgtgc agttgctggt gttccgcatc accgacatgg
     1381 tgctgcaggg cctgcccgag cgcattcacg agggcgaggt cgccgcagcg gccctgctgg
     1441 tgggcgccaa gctggccaca gccctggtcc tggccgccgc cgtggccggc tgatccggaa
     1501 aggcgagggc caggtcccgt gcattttccc aggctcccag actggctggt ctatgccgcc
     1561 gccgtcagcg ccctgctgat cgtcgcggtc ggccggcagg agcgctccga cgccccgccg
     1621 cccccgccgc ctgtgccggg cgaggagggc ctgccgctgg ggccggcctc gccgttcgac
     1681 ccctccatcg tggtgcaggt gcccaacacg cccgagcccg gctcgggcac cgccttttcg
     1741 gttggcgatg acggcacctg gctgaccgcc cgtcatgtgg tcgatggctg caagtcggcc
     1801 gccatcgtcg tggctgacgg acgcggggtc gcggccagga tcaccatcga cccccgcgcc
     1861 gacgccgcca tcctgaccac cgagggcggg gcgccggccg tgcccctggg gctggaagag
     1921 ccgctgcgcc gcggcgcccg cgcctatcat cccggcttcc cacagggcca tccgggcgag
     1981 gccgcctcgc gcctgctggg tcgcgagaac ctggtcgttc acggtcgcgg ggcccgcacc
     2041 gagccggtgc tggtctgggc cgaggtcggg cgcaccgaca ccctgaaggg cacactggcc
     2101 ggtctttcag gcgctccggc cctcgacagc gccgggcgcg taatcggcgt cacggtggcc
     2161 gaaagccccc gccggggccg catctacacc accgcccccg agaccctgaa aacccttctg
     2221 ggcccgcgcc gccgctccgg cgccgatatc gccatgggtg aagcgatcac caccgagaac
     2281 tatggccgcg tcgccgatgg cctgcggcgg gacctgcgcg tggcgcaggt ggtttgcctg
     2341 ggggtctagg tttcaggtcc tccccctgac gggggaggac ttctaagccg gcttggcggc
     2401 cgcctcttcc gcctcggctc gccgatcaat ctccccctgc ctcgccgcca ccatgaacga
     2461 cagcacaatg gccgccaccc cgatcagggc ggtgtagagg aagaaggccg tatagcccgc
     2521 cgccatggcc ggggcgctga cgcccagctt ggcgccggcg gcggtgaagc tttcgggcgg
     2581 caggcgggtg aacaggccct tcagggcgcc gatggggccg ccggcttcgg ccgcgatggc
     2641 cgaggcctcg acaatgcggc ccgactgcga ggcgatcagc ttgccgggca gggcgtagag
     2701 cgacgagaac agggcgtatt gcgtggccgt gaagcccacc gtcgtcaggc tggacatata
     2761 ggcgatcagg gcggtgccgg cgacgccgcc agccacgttg tcgatgcaga tcgcccagaa
     2821 cagcaccggc acattgtggc cgctcatggc cagccaggcg aagaacaggt tgctgatggg
     2881 ttgggcgaag gcgccgatca ccagggcctt catcaggccc agccgcgcta cgatcacccc
     2941 gcccaggaac accccgatca tcgaggcgat gatgccgaac accttccgga tttcggcgat
     3001 ctcggtcaga gtgaagccca tgtcgcgata gaacgggttc atgatgttga gcacgaagtc
     3061 cgacacccgg tagacgcaga tcgccgccag gatcaggccc gccacgccgt gaaagcgcct
     3121 ggtgaagtcg atcagcgggt cgccgaacgc cgccgacatg tagacgccag gcttggtgcg
     3181 caccttgggg atcggccatg cggccaccaa gacgatggcc agacccgtta gcacgcccag
     3241 aagctgcagc cagacgccgt tcggcttggc cttccaggct tcggctaggg cctcaccgcc
     3301 cacgagcttg gacagcaggg atgcatcgcc cgacagccct gaacccagcg ccaaggcgcc
     3361 gaccagcagg atgcccaggc ggacgatcca ctccaggatt tcgagagggc gggactggtt
     3421 gacgtcgccg gtcgggatcg gccgaacggt gtgctcggcc tcgcgcgggg cccacagggt
     3481 ggccatcacc ccgataccaa cgacgaagcc catgacggca taggagaagt tccagccaaa
     3541 gcgctcggcc aacagcaggg ggccggcgcc ggcggcgatc atcgcgcccc ggtagcccca
     3601 ctgataggcc acagccatgg gcccctgttc ttccttgtcg gcgacttcga tccgccaggc
     3661 gtcaaggact acgtcgtgcg aggccgtggc gaaaccgacc gcgaccgcaa cggcggccag
     3721 aattcccaac tgtgaggccg ggttgagcgt cgagatcgtc cacagaccga ggatcatcgc
     3781 gatctggaaa acaaggatcc aggcccgccg gtggcccatc agcttgtgca gcaccggcac
     3841 tctggcgcgg tcgatcagcg gggcccacag gaacttgaac gagaacgaga gggtggccag
     3901 gctgaagaag ccgatcaccg ccagcgacac gccggagtcg cgtagccaca gcgacagggt
     3961 gtcgaagatc agcatgaacg gcaggcccga ggcaaagccc agacccagca tcgccaggct
     4021 gcgacgctcg ccgaagaagg ccagggtctg aagaagagtc ttcttctgcg gcttggctgc
     4081 gggctggtcg gtcatgcggc gtcccctcgc ggggaacgcc ccgcgcttta gccggcgacc
     4141 ttggcccgcg acgcggtttt cgtccagacg tccggggcgc cggctcccgt ccagcgggtc
     4201 agcacatcgc gcagggcggc atgggtcagg ggcttgacca gaaagtcggt catgccggcc
     4261 gcgaggcagg cgcggcggtc ctcgtcgaag gcgtcagcgg tcagggcgac aatgggcgtc
     4321 gccacgccct gagcgcgcag gcggcgggtg gcgtcgatgc cgttcagcac aggcatgcgc
     4381 aggtccatca agatcagatc gt
//
LOCUS       DAOMEO010000109         4397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2428_length_4397_cov_2.455781, whole genome shotgun sequence.
ACCESSION   DAOMEO010000109 DAOMEO010000000
VERSION     DAOMEO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_4397_cov_2.455781"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..3561
                     /locus_tag="PLH31_03565"
     CDS             487..3561
                     /locus_tag="PLH31_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88342.1"
                     /translation="MTNQRRKAVLTRGLAVALLAGASQVALTTGAFAQDATQVEEVVV
                     TGVRGSQIKSVDVKRTQASIVDAISAEDIGKLPDVTIADSLQRISGIQVQRNAGEGTT
                     VNIRGLAQVITLLNGEQYLSAGNMGSAQPNLLDVPSQLMNQVLVYKSTDPKNALSGIS
                     GTIDLRTRRPFQFGEDLTFTGTAEAQRGERTKENDYLVNGVLNWRNDRVGLMVSAAKS
                     KSNLGNNYSGTVGLSGNNDWGGNFPNSYISPHGYESFNRVVERDRLGVNVAFEADLGE
                     GFTLIAEGFYAELEEYNRAVGLNISNRWDGGAFGAWTKPTVSSPVGIGNWQTVDEYDI
                     DAWWINSFTVNRTTKSKTKNYNLELNYDNGGPFTFETRAIRADGERLSMNGQAQGDLS
                     NWRYAPGRFNLFRDANDRTRGTFYPKAICDKFPTSRRTNAVVGSAGGCYLDPNPQGYG
                     ANPQLNYNIAGDHPVWSGFDKPLVGGLGAGKTLKDYMANKDSYAIGAFSSEGNNEVES
                     DMNVFRADGHYEFDDKFLGFITKVDAGVRQSERSVDVEQFHLFSGFYGGSPGAVQANG
                     TPVPAGGCEAQWKAIDVVMSQNQCQAGEFVPDPVTGLQVFQGYTVNAPTRLDTYNNVV
                     WVDDLGGVTSGVPGFWAIDPRDFDDAEAFHKKVFGGAIRVQIPGQTYDVSLREQSAFG
                     AANFEMGGLSGNFGLRVIETELTVKQNLTGDTRNYGDTNADAGDNVTKRKYTDWLPSL
                     NLTYNLGENIKFRAAYSKTMQPLDLGNYGGGLSIFTSDCGAALPNVRCVTGASASGNP
                     NLDPWRSQNFDGAIEYYFGRASMLNISAFKLKIDSFVTNGTTTGRFPDQDGVIRRDVN
                     VTLPIQGDGGEVEGLEVGAKLAMSDIMPDAGLLSNFGVDSNYTYSPSEESRVGLDGEK
                     LSFVDNSKHQFNLVGWYQDEKLQARVAYNYRTDRLSSVMSNNIPIYQDSTGYVDVNVS
                     YNVRDNITVYFNGSNVTGEIEDYYAKFAKGKTQYISQNEFEPRYTIGLRARW"
     gene            3705..>4397
                     /locus_tag="PLH31_03570"
     CDS             3705..>4397
                     /locus_tag="PLH31_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR88343.1"
                     /translation="MDQAAQDNRIRSVVIVGGGTAGWMAAASLQRHFQGSPLKITLIE
                     SSEIGTIGVGEATIPTIRNFYQNLGLSDLDVMRATGATCKLGIRFNDWLKPGSSFLHP
                     FGLFGQDLRGVPFHHYWLRLKALGDESEIGEYSLGASLAKGGKFTQPSRNPPSSLSVF
                     DWALHLDASLFAKLMRQVAENAGVKRIDARITRVNLRPEDGFIASVDLDTGATVEGEL
                     FIDCSGFRGLLIE"
BASE COUNT          868 a         1397 c         1346 g          786 t
ORIGIN      
        1 tggtgttttc cgcgtggaaa ggcgcaagcg caaggcgctg aacgcgctcg gcgtgcgaag
       61 tccggaaacc gttggtccgc ttgatgtttc atgccctaag gcgacttcgg cccattgtag
      121 atggcggttg aaccctcggg aaaggttggc attttttcga aaatatggtt gcggtaacac
      181 tggggtttcc cccgggcgtc gctcgcggat ccggcgcgca aaagaccgcg cgagacaaga
      241 atgacaacgt tgacattgtt gttgacttga tgcggacatt tgatgatgtt gccctcaacc
      301 gataacggcg ttgggccgga atcgacagcc cgggcgctgg gggtcgccgt cacctacgga
      361 catagcggac aaacggttcg ccgggcctct gaggcgctca atcggttctg gatcaccccg
      421 cgcagacggg gcggccagga acggggcgga caatcaaata aaaatgttag ttggggagct
      481 aggaatttga ccaatcaacg caggaaagcc gtgcttacgc gcggtctggc ggtggccttg
      541 ctggccggcg cctctcaggt ggcgctaacc accggcgcat tcgctcagga cgccacgcaa
      601 gtcgaagagg tggtcgtcac gggggtccgc ggatctcaga tcaagtcggt cgacgtcaag
      661 cgcacgcaag cctcgatcgt cgacgccatc tcggcggagg acatcggcaa gctgcccgac
      721 gtcaccatcg ccgactcgct gcagcgcatc tcgggcattc aggtccagcg caacgccggc
      781 gagggcacca cggtcaacat ccgcggtctg gcccaggtca ttaccctgct gaacggtgaa
      841 cagtatctca gcgccggcaa tatgggctcg gcccagccga acctgctgga cgtgccgtcg
      901 cagttgatga accaggtgct ggtctacaag tcgaccgatc ccaagaacgc cctgtcgggc
      961 atttcgggca ccatcgatct gcgcacgcgt cgtcctttcc agttcggcga ggatctgacc
     1021 ttcacaggca cggcggaagc ccagcggggc gagcgcacca aggaaaacga ctacctggtc
     1081 aatggcgtcc tcaactggcg caatgaccgc gtcggcctga tggtctcggc cgccaagagc
     1141 aagtcgaacc tcggcaacaa ctattcgggc accgtcggcc tgtcgggcaa caatgattgg
     1201 ggcggcaact tccccaacag ctacatctcg ccccacggct acgagagctt caatcgcgtg
     1261 gtggagcgtg atcgcctggg cgttaatgtc gcctttgagg ccgatctggg cgaaggcttc
     1321 accctgatcg ccgagggctt ctacgccgag ctcgaggaat ataaccgcgc tgtcggtctc
     1381 aacatctcca accgctggga cggcggcgcc tttggcgctt ggaccaagcc cacggtgtcg
     1441 agcccggtcg gtatcggcaa ctggcagacg gttgacgaat acgacatcga cgcgtggtgg
     1501 attaactcct tcaccgtcaa ccgcaccacc aagtccaaga ccaagaacta caacctcgaa
     1561 cttaattacg acaacggcgg tccgtttacc ttcgagacgc gcgccatccg cgcggacggc
     1621 gagcgtctga gcatgaacgg tcaggcccag ggcgacttga gcaactggcg ttacgcgccg
     1681 ggccgtttca atctcttccg cgacgccaac gaccgcaccc gcggcacgtt ctatcccaag
     1741 gccatctgcg acaagttccc gacttcgcgc cgcacgaacg ctgtcgttgg cagcgccggt
     1801 ggttgctatc tggatccaaa cccccagggc tacggcgcca atccgcagct aaactacaat
     1861 atcgcaggcg atcaccctgt gtggtcaggc ttcgacaagc cgctggttgg tggtcttggt
     1921 gcgggcaaga ccctcaaaga ctacatggcc aacaaggata gctatgcgat cggggccttc
     1981 tcatctgaag gcaacaacga agtcgagtcg gacatgaacg tgttccgcgc cgatggtcac
     2041 tatgagttcg acgacaagtt cctgggcttc atcaccaagg tcgacgcggg ggttcgccag
     2101 agcgagcgtt cggtcgatgt cgagcagttc cacctgttct cgggcttcta cggcggttcg
     2161 cccggcgcgg ttcaagccaa cggtacgccg gttccggccg gcggctgcga ggcccagtgg
     2221 aaggccatcg acgttgtcat gagccagaac cagtgccagg ctggcgagtt cgtgcccgat
     2281 cccgttaccg gtctacaggt gttccagggc tacaccgtca acgcgccgac ccgtttggac
     2341 acctacaata acgtggtctg ggtcgatgac ctgggcggcg tcaccagtgg tgtccccggc
     2401 ttctgggcga ttgacccgcg cgacttcgac gatgcggaag ccttccacaa gaaggtcttc
     2461 ggcggcgcca tccgcgttca gatccccggc cagacctatg acgtcagcct gcgcgagcag
     2521 agcgcgtttg gcgcggccaa tttcgagatg gggggtctgt cgggcaactt cggcctgcgc
     2581 gtcatcgaga ccgagctgac ggtgaagcag aacctcaccg gcgacacccg caactacggc
     2641 gacaccaatg ccgacgccgg cgacaacgtc accaagcgca aatataccga ctggctgccg
     2701 tcgctgaacc tgacctacaa cctcggcgaa aacatcaagt tccgcgcggc ctactccaag
     2761 accatgcaac cgctggacct gggcaactac ggcggcggtc tgtcgatctt cacctccgac
     2821 tgcggcgcgg ccctgccgaa cgtccgctgc gtgactggcg ccagcgcctc gggcaacccg
     2881 aacctggatc cgtggcggtc gcagaacttc gacggcgcca tcgagtacta tttcggtcgc
     2941 gcctcgatgc tgaacatctc ggccttcaag ctgaagatcg acagcttcgt gaccaacggc
     3001 accaccaccg gccgcttccc cgaccaggac ggcgtcatcc gtcgcgacgt caatgtcacc
     3061 ctgcccatcc agggcgacgg cggcgaggtc gagggcctgg aagtgggcgc caagctggcc
     3121 atgagcgaca tcatgccgga cgccggtctg ctgtcgaact tcggcgttga cagcaactac
     3181 acctactcgc ccagcgagga gtcgcgggtc ggcctggatg gcgaaaagct gtccttcgtc
     3241 gacaactcca agcaccagtt caacctggtg ggttggtatc aggacgagaa gctgcaggcg
     3301 cgcgtcgcct acaactatcg aaccgatcgt ctgtccagcg tgatgagcaa caacatcccg
     3361 atctatcaag acagcacggg ctatgtggac gtgaacgtca gctacaacgt tcgtgataat
     3421 atcacggtct acttcaatgg ttcgaacgtg accggcgaga tcgaggatta ctacgccaag
     3481 ttcgcgaagg gtaagactca gtacatcagc cagaacgagt tcgaaccccg ctacaccatc
     3541 ggccttcggg cgcgctggta acctctaggc tcacctcctg cggccgccgt gaccccccgc
     3601 gtcacggcgg ctcctttttg gaggcggacc tgtccgagac gggcgccagg cggtagcctc
     3661 gcggtcagcc gggccagata tgaaagtgct tcgggagggc gacggtggat caagcagccc
     3721 aggacaaccg cattcgcagc gtcgtgatcg tgggcggcgg gacggccggc tggatggcgg
     3781 cggccagtct gcagcgccac ttccagggct cgcccctgaa gatcaccctg atcgagagtt
     3841 ccgagatcgg aaccatcggg gtgggcgagg cgaccatccc gactatccgc aatttctacc
     3901 agaacctggg tctaagcgac ctggatgtga tgcgggccac cggcgccacc tgcaagctgg
     3961 gcattcgctt caacgactgg ctcaagccag gctcatcctt cctccatccg ttcggcctgt
     4021 tcggccagga tctgcggggt gtgccattcc accactactg gctgcggctg aaggccctgg
     4081 gcgacgagtc cgagattggt gaatactcgc tgggcgcatc cctggccaag ggcggcaagt
     4141 tcacccagcc atcgcgcaac ccgccctcca gcctgtcggt gttcgactgg gcgctgcacc
     4201 tggacgccag cctgttcgcc aagttgatgc ggcaggtcgc cgaaaacgcg ggggtcaagc
     4261 gcatcgacgc ccggatcacc agggtcaatc tccggcctga ggacggcttc atcgcctcgg
     4321 tcgatctcga caccggcgcc acggtcgaag gcgaactctt catcgactgc tcggggttcc
     4381 gcggcctgct gatcgag
//
LOCUS       DAOMEO010000110         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2444_length_4367_cov_2.292672, whole genome shotgun sequence.
ACCESSION   DAOMEO010000110 DAOMEO010000000
VERSION     DAOMEO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2444_length_4367_cov_2.292672"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PLH31_03575"
     CDS             <1..1515
                     /locus_tag="PLH31_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR88344.1"
                     /translation="VPAHAQHVTVGKLAPATADADGIRAWKGLPYAAPPVGDLRWKPP
                     APVVPWTGERATDRFGPNCAQPKVYNDIDPYTPSMSEDCLYLNVWSPAKAGESLPVFF
                     WIHGGGYGAGSGAEPRHDGVALAKKGVVVVTINYRLGAFGFFAHPDLTRESPNRASGD
                     QALADMIAALRWVQANIALLGGDPDRVTIAGESAGSDAVSRLMTSPQAKGLFQRAIGQ
                     SGSAFGTMGRDDTLAMGEANGLAFGRALGGDSLAALRRRTTAEILATWTAPNPRFAFR
                     PILDGWILPKGAAETFAAGRQSDVPLLVGWNLDEGSLLEGGVFAGRNLPEALKMTFGN
                     RAAQAAKLYPHDTPEQMIRSQINFAGDAWMGLPTWSWATAQARTGKAPVWAFRFDHAP
                     KTPDGWFGEGNRGKSLGAFHSAEIVYVFNHPDNGGAWAVDDVDRRVADLMSSYWANFV
                     KTGDPNGPGLPVWPVYAPDGDARKLIIDADARVIADDDQPRLKFLHSQAARPGP"
     assembly_gap    1680..1689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1690..>2075
                     /locus_tag="PLH31_03580"
     CDS             <1690..>2075
                     /locus_tag="PLH31_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HQR88345.1"
                     /translation="SAAPIRLNQLGVEAAGPKRAVLSDPSIAPLPWTLRNGKGAIVAS
                     GKTRVFGADASSGEHVHQIDFSGFKGSGDGYRLSVGAAQSRPFAIGGRLYGALKADAL
                     GFFYQNRASIPIEARFVQRPDLARPA"
     assembly_gap    2076..2085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2086..3484
                     /locus_tag="PLH31_03585"
     CDS             <2086..3484
                     /locus_tag="PLH31_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HQR88346.1"
                     /translation="KVTCFNKADERGQVWAGCDYSLDVSRGWYDAGDHGKYVVNGGIS
                     VWTLLNAHERAAVLGKTSTLAAFADGRGRIPENANGVSDLLDEARWEMDFLLAMQVPQ
                     GTTQALPVGQQRVGPTGRLVLTDLDASGMAHQRMGDERWTALPMAPADDKETRYLYHP
                     TTAATLNLAATTAQAARIWKSIDPAFSARCLDVAKRAYAAARRNPEVYALGAFTGSGG
                     YGDGNVEDEFYWAAAELYATTGAAEYEAALRASPLFLAGPSAGGKATGDIGWPSVGAL
                     GTITLALAPNGLKPAEIAMARANLAASADRYLADGAGEGYALPYAPKGYPWGSTSSVL
                     NRAMVLGLAADFTGKTAYRNGAVDAMDWILGRNPLDRSFVTGHGARPMVNPHHRFWAR
                     QADARYPAPPPGVVSGGPNSTNMGDPVAETLKGKCAPQTCWTDDYRAFTQNEVAINWN
                     APLLWVATWLDEPTR"
     gene            3686..>4367
                     /locus_tag="PLH31_03590"
     CDS             3686..>4367
                     /locus_tag="PLH31_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR88347.1"
                     /translation="MAVQAAAAIGVTLISRAEERPFHVAWLVVVLTLLAMRVVADRML
                     GAALAGRFLRDRLWVLARIQSLGLTLSAGLWALLACLRIPVDSPVTRYALIVVLSALA
                     GGAVGVLSPLKWTGRLYVSLILIPASVTLVILGGADATLGALGGVFWVVMMVGHNNNH
                     MLLVDCLRLRDENHELLQNIQRRNHDTRRLNRDLESRVRARTQQLEKLSEEAQVANRA
                     KSQFLAMVS"
BASE COUNT          696 a         1549 c         1436 g          666 t
ORIGIN      
        1 gtccctgccc acgcccagca cgttaccgtc ggcaagctcg cgcccgccac ggcggacgcc
       61 gatgggatcc gggcgtggaa gggcctgccc tatgccgccc cgcccgtggg cgatctgcgc
      121 tggaagccgc ccgcgcccgt cgtcccgtgg acaggcgaac gggcgaccga caggttcggg
      181 cccaactgtg cccagcccaa ggtctataac gacatcgacc cctacacccc gtcgatgtcc
      241 gaagactgcc tgtatctgaa cgtctggagc ccggccaagg cgggcgagag cctgccggtg
      301 ttcttctgga tccatggcgg cggctacgga gcaggctcag gcgccgagcc gcgccacgac
      361 ggcgtcgccc tggccaaaaa gggtgtggtg gtggtgacaa tcaactaccg gctgggcgcc
      421 ttcggcttct tcgcccatcc ggacctcacc agggaatctc ccaacagggc cagcggcgac
      481 caggccctgg ccgacatgat cgcggccctg cgctgggtgc aggccaatat cgccctgctg
      541 ggcggcgatc ccgatcgggt gacaatcgcc ggcgagtcgg cgggctccga cgccgtcagc
      601 cggctgatga cctcgcccca ggccaagggc ctgttccagc gcgccatcgg gcaaagcggc
      661 tcggccttcg ggaccatggg cagggacgac acgctggcga tgggcgaagc caacggcctg
      721 gcctttggtc gcgccctggg cggcgacagc ctggcggccc tgcgtcggcg caccacggcc
      781 gagatcctgg cgacctggac ggcgccgaac ccgcgctttg cgttccggcc catcctggac
      841 gggtggatcc tgcccaaggg cgcggccgag acctttgccg ccggccgaca gagcgatgtg
      901 cccctgctgg tcggctggaa ccttgacgag ggcagcttgc tggaaggcgg cgtgttcgca
      961 ggccgcaatc tgcccgaggc gctgaaaatg accttcggaa accgcgccgc ccaggccgca
     1021 aaactgtacc cccacgacac gcccgagcaa atgatccgct cacagataaa cttcgccggc
     1081 gacgcctgga tgggtctgcc gacctggtcc tgggccacgg cccaggcgag gacaggcaag
     1141 gcgccggtct gggccttccg ctttgatcac gcgcccaaga cgcccgatgg ctggttcggc
     1201 gagggcaatc gcggcaagtc gctgggcgcg ttccattccg ccgagatcgt ctatgtgttc
     1261 aatcaccccg acaatggcgg cgcgtgggcc gtcgacgacg ttgaccgccg ggtggccgat
     1321 ctgatgtcgt cctactgggc caatttcgtg aagaccggcg atcccaacgg ccctgggctt
     1381 ccggtgtggc cggtctatgc gcccgacggc gacgcccgca agctgatcat cgacgccgac
     1441 gcccgcgtga tcgccgacga cgaccagccg cgcctgaagt tccttcacag ccaggcggcc
     1501 cgcccgggac catgacggca atttctcagg cgacaacgct gtcaggttac cgctaacttg
     1561 ccggggaaga cgcgcgctca cgacgcgtcg caggcaggaa gacgcgtatg accaggatca
     1621 ccccggccct gacggccatt gccctgaccc tcgccttgag cggtctggcc caagccgacn
     1681 nnnnnnnnng agcgctgcgc ccatccgtct caatcagctg ggcgtggagg ccgcggggcc
     1741 caagcgcgcc gtgctgtccg atccctcgat tgcgcctctg ccctggactc tgcgtaacgg
     1801 caaaggcgcg atcgtcgcca gcggcaagac cagggtgttc ggcgccgatg cttcgtcggg
     1861 cgagcacgtt catcagatcg acttcagcgg cttcaagggc tcgggcgacg gctatcgcct
     1921 cagcgtcggc gcagcccaga gccggccctt cgcgatcggc ggccggctgt acggcgcgct
     1981 gaaggctgac gccctgggct ttttctacca gaaccgcgcc agcattccga tcgaggcccg
     2041 gttcgtgcag cgccccgacc tggcccgccc cgccgnnnnn nnnnncaagg tcacctgctt
     2101 caacaaggcc gacgagcgcg gtcaggtctg ggccggctgc gactacagcc tcgatgtcag
     2161 ccgcggctgg tacgacgccg gcgaccacgg caagtacgtc gtcaatggcg gcatctcggt
     2221 ctggacgctg ctgaacgctc acgaacgcgc ggccgtgctc ggcaagacct cgaccctggc
     2281 cgccttcgcc gacggcaggg gcaggatccc cgagaatgcc aatggcgtct ccgacctgct
     2341 ggacgaggcc cgctgggaga tggacttcct gctggccatg caggtcccgc aaggcacgac
     2401 ccaggccctg ccggtgggcc agcagcgggt cgggcccacc ggccggctgg tcctgaccga
     2461 cctggacgcc tcgggcatgg cgcaccagcg catgggcgac gagcgctgga cggcgctgcc
     2521 gatggctccg gccgatgaca aggaaacccg ctacctctat cacccgacca ccgccgcgac
     2581 cctgaatctg gccgccacca ccgcccaggc cgcgcggatc tggaagagca tcgacccggc
     2641 cttctcggcc cgctgcctgg atgtcgccaa acgcgcctac gccgccgcca ggcgcaaccc
     2701 ggaggtctac gccctgggcg ccttcaccgg cagcggcggc tatggcgatg gcaatgtgga
     2761 ggacgagttc tactgggcgg ccgccgagct gtacgccacc accggcgcgg ccgagtacga
     2821 ggccgccctg cgcgcctcgc cgctgtttct ggccgggccc tcagccggcg gcaaggcgac
     2881 cggcgatatc ggctggccct cggtcggcgc tttggggacc atcaccctgg cgctggcgcc
     2941 caatggcctg aagccggccg agatcgccat ggcccgcgcc aacctcgccg ccagcgcgga
     3001 tcgctatctg gccgatggcg ccggcgaagg ctacgccctg ccctatgccc ccaagggtta
     3061 tccctggggc tcgacctcat cggtgctgaa ccgggccatg gtgctgggcc tggccgccga
     3121 cttcaccggc aagaccgcct atcgcaacgg cgccgtagac gccatggact ggatcctggg
     3181 tcgcaacccc ctggaccgct cgttcgtcac cggccatggc gcgcggccca tggtcaaccc
     3241 gcaccaccgc ttctgggcca gacaggccga cgcccgctat cccgccccgc cgcccggcgt
     3301 ggtctcgggc gggccaaact cgaccaacat gggcgacccg gtggccgaga ccctgaaggg
     3361 caagtgcgcg ccccaaacct gctggacaga cgactatcgc gccttcaccc agaacgaggt
     3421 ggccatcaac tggaacgcgc cgctgctgtg ggtggcgacc tggctggatg agccaacacg
     3481 ctagcgtccg gcgccgcagg cagggctacg cgacactttg tcgcttggaa tgggataact
     3541 tcgttcatta tcggagccat agtgctgaaa gggattttca gggacgctgg cgagaatgca
     3601 ctcggacaga gcagaggata agccacgggg cgacgcccta aggcttgccc tgctgggaca
     3661 cgcgcatcgc aacagcctgg cgaccatggc ggtccaggct gcggcggcca tcggcgtcac
     3721 cctgatctcg cgggctgaag agcgcccctt ccatgtggcc tggctggtcg tcgttctgac
     3781 cctgctggcg atgcgggtcg tggccgatcg catgctgggc gccgcgctcg cggggcgctt
     3841 cctgagagat cgactgtggg tgctggcccg cattcaaagc ctgggcctga ccctcagcgc
     3901 cgggctgtgg gccttgctgg cctgcttgcg cattcccgtc gacagccccg taacccgtta
     3961 tgccctgatt gtcgtgctgt cggccctcgc cggcggggcg gtcggcgttc tgtcgcccct
     4021 gaaatggacg ggacgcctct atgtctcgct gatcctgata ccggcctccg tgaccttggt
     4081 catcctgggc ggcgctgatg cgaccctggg ggccctgggc ggcgtattct gggtcgttat
     4141 gatggtgggc cacaacaaca accacatgct tctggtcgat tgcctgcggc tgcgcgacga
     4201 aaaccacgag ctgctgcaaa acatccagcg gcgcaatcac gacacccgcc ggctcaaccg
     4261 cgatctggaa agccgcgtcc gggcccgcac ccagcaactt gaaaagctgt cggaagaggc
     4321 ccaggtcgcc aaccgcgcca aatcacagtt cctggcgatg gtcagcc
//
LOCUS       DAOMEO010000111         4362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2445_length_4362_cov_2.193870, whole genome shotgun sequence.
ACCESSION   DAOMEO010000111 DAOMEO010000000
VERSION     DAOMEO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_4362_cov_2.193870"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1084
                     /gene="hemH"
                     /locus_tag="PLH31_03595"
                     /pseudo
     CDS             65..1084
                     /gene="hemH"
                     /locus_tag="PLH31_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
     gene            1095..1572
                     /locus_tag="PLH31_03600"
                     /pseudo
     CDS             1095..1572
                     /locus_tag="PLH31_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
     gene            1845..3269
                     /gene="rho"
                     /locus_tag="PLH31_03605"
     CDS             1845..3269
                     /gene="rho"
                     /locus_tag="PLH31_03605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQR88348.1"
                     /translation="MTEDTQHDASSEEAIVDTTAAAEAPGVQNASTESDDAGDAEDTG
                     IADAVAALGLKTMSLQELKEKSPADLLAFAETFEVENANSMRKQDMMFAILKTLAEEG
                     VEISGSGTMEVLQDGFGFLRSPEANYLPGPDDIYVSPSQIRKYGLRTGDTVDGAIRSP
                     REGERYFALTGVAKINFEAPDNVKHKIHFDNLTPLYPEERLNMELPDPTVKDRSGRII
                     DIVAPLGKGQRCLIVAPPRVGKTVMLQNIAKSIETNHPECYLIVLLIDERPEEVTDMQ
                     RTVKGEVIASTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSVTRLGRAYNTTV
                     PSSGKVLTGGVDANALQRPKRFFGAARNVEEGGSLSIIATALIDTGSRMDEVIFEEFK
                     GTGNSEIILDRKVADKRIFPAIDVLKSGTRKEELITPRDQLQKTYVLRRILNPMGASD
                     AIEFLLEKMRQSKTNGDFFQSMNT"
     gene            complement(3350..4315)
                     /locus_tag="PLH31_03610"
     CDS             complement(3350..4315)
                     /locus_tag="PLH31_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HQR88349.1"
                     /translation="MLAIQAVRAGGPEVLEAIDLPVPAPGPGQILVRHHAVGLNFIDT
                     YHRSGLYPMKMPAVLGLEAAGIVEALGEGVTRFVAGDRVAYNGSLGAYAEAAVVPAER
                     AVKVPDSVSLDVAAAVLLKGMTAEFLVRRCFHVKPGDIVLVHAAAGGVGSILVQWCKT
                     LGATVIATVGSVAKAAIARELGADHVILYGHEDVAARVAEITAGKGVAVVYDGVGKDT
                     FEASLKSLARRGVLATFGNASGPVPPFSPLELSGKSLFVTRPRLFDYIVTTEELDESA
                     QALFAVLASGAVKIDIGQTFPLSEARAAHEALEGRRTTGATLLLP"
BASE COUNT          781 a         1456 c         1370 g          755 t
ORIGIN      
        1 acgggatcct accggatacg ccgatcgccc atgttgaaca ggttctggcc cgggtgactg
       61 gtaaatgagt tcgcgcagga aaatcgccgt cgttctcttc aatctgggcg gaccagatgg
      121 cccggacgcc gtccggccgt tcctgttcaa tctttttcgc gaccccgcga tcatcggagc
      181 gccggccttc atccgctatc cgatcgccgc cctgatctcg acgacgcggg aaaagagcgc
      241 caaggccaat tacgccatga tgggcggcgg ctcgcccttg ctgcccgaga cccagaagca
      301 ggcgcaggcg ctcgaatccg ccctggccca ggctctgccg gacgccgaga ccaggtgctt
      361 cattgccatg cgctactgga agccgctgac cgaggagacc gccagggccg tcaaggcctg
      421 ggcgccggac gaggtcgtgc tgctgccgct ctatccgcag ttttccacca ccacgaccgg
      481 ctcgtcggtc aaggcgtgga acaaggccta tcgcggcccc ggcaaggttc gcaccgtctg
      541 ttgctatccg accgacgacg ctttgattga cgcccatgcg cgcctgatcc gcgagaccta
      601 tgagaaggcc ggaagcccca aatcggcgcg cctgctgttc tcggcccatg gcctgcccga
      661 aaaggtcatt ctcgccggcg atccctatca gaagcagatt gaggccacgg ccgccgcggt
      721 cgccgcccgt ctgggcgatg gctgggactg gaaggtcacc tatcaaagcc gcgttggccc
      781 gttgaagtgg atcggtccct ccaccgacga tgaaatcctc gccgccagcg aagagggtct
      841 ggccctggtg gtgacgccca tcgcctttgt ctccgagcat atcgagaccc tggtcgagct
      901 ggatcacgaa tatcgcgagc tggcggtcga ggccggctgc ccggcctatg tccgcgttgc
      961 ggccctgggc atcgagccgg tctttatcga cggcttgacc agggcggtgg ttgaggccct
     1021 gccgcgcgag ggcgtggcgc ccgcttgtgc gtggcgctgt gcgggtgacc tgaggcagtg
     1081 tccccgtacc cgggatgtcg ctgcgtgacg gatttcctgg ttcagaactt caatgtcttt
     1141 cgcggcctgc acattctggc cgtgatcgcg ttcatggccg gcatgctcta tctgccgcgt
     1201 ctcttcgtct accacaccaa ggctacgccc ggttcggaga tggacgagac cttcaaggtc
     1261 atggagcgcc ggctgcttcg cggcatcatc aatccagcct cgatcctgac cttcgtcttt
     1321 ggcctggccc tgatcgtcgc cgacagccag atccgcggct ggagctttct gatgcagccc
     1381 tggatggcga ccaagctggc ggccctggtt ggtctctatg ggtggcacgg tttcctgtcg
     1441 gccgcccgcc gcaagttcga gcgcggggaa aacacccgct cggaaaagtt ctggcggatc
     1501 accaacgaga tcccgttcgt gctggcgatc atcatcgttc tgtcggtgac gaccaagttc
     1561 ctggaccact gatcatgggt cagcgggggc ggcgcagctt gacccgctct cccgcatgtg
     1621 gttcccatca attccaagtc tgacgctgag cgtttgacct gtttcttcac ggaccggcgc
     1681 ccgtcctggt gcgcccccgg agccaagctt cgaaggtctg ttccccaatc gatccgcgtc
     1741 gtcgtcatga cgcgctcgtc tggcctcccg gcctgacgtg agaactgaag tccctttccg
     1801 gccggatcgc ctctcgcggt ccgcgccccc gtttgagtct gatcatgacc gaagacaccc
     1861 aacacgacgc ttccagtgag gaagccatcg tcgacaccac ggccgctgct gaggcacctg
     1921 gcgtccagaa cgcctcgacc gagtccgacg acgccggcga cgccgaagac accggcatcg
     1981 ccgacgccgt tgcggccctg ggcctcaaga ccatgtccct gcaggagctg aaggagaaat
     2041 ccccggccga cctgctggcc ttcgccgaga ccttcgaggt cgagaacgcc aactccatgc
     2101 gcaagcagga catgatgttc gcgatcctga agaccctcgc cgaagaaggc gtggagatct
     2161 cggggtcggg caccatggaa gtgctgcagg acggctttgg gttcctgcgc tcgccggaag
     2221 ccaactatct gccgggtccg gacgatatct atgtttcgcc ctcgcagatc cgcaagtatg
     2281 gcctccgcac cggcgacacc gtcgacggcg ccatccgctc accgcgcgaa ggcgagcgct
     2341 actttgccct gaccggcgtg gccaagatca atttcgaagc cccggacaac gtcaagcaca
     2401 agatccactt cgacaacctg acgccgctct atcccgaaga gcgcctgaac atggaactgc
     2461 ccgatcccac cgtgaaggat cgctcggggc ggatcatcga tatcgtcgcc ccgctcggca
     2521 agggccagcg ctgcctgatc gtcgccccgc cgcgcgtggg caagaccgtc atgctgcaga
     2581 acatcgccaa gtcgatcgag accaatcacc ccgagtgcta cctgatcgtg ctgctgatcg
     2641 acgagcgccc ggaagaagtc accgacatgc agcgcaccgt gaagggtgag gtcatcgcct
     2701 cgaccttcga cgaaccggcg acccgccacg tgcaggtggc cgagatggtc atcgaaaagg
     2761 ccaagcgcct ggtcgagcac aagcgcgacg ttgtgatcct gctggactcg gtgacccgtc
     2821 tgggccgcgc ctacaacacc accgtcccgt cgtcgggcaa ggtgctgacc ggcggcgtcg
     2881 acgccaacgc cctgcagcgt cccaagcgct tcttcggcgc ggcgcggaat gtggaagagg
     2941 gcggttcgct gtcgatcatc gccaccgccc tgatcgatac gggcagccgt atggacgaag
     3001 tgatcttcga agagttcaag ggcaccggta actcggaaat catcctggac cgcaaggtcg
     3061 ccgacaagcg catcttcccg gccatcgacg tgctgaaatc cggcacccgc aaggaagagc
     3121 tgattacccc gcgcgaccag ctgcagaaga cctatgtgct gcgccgcatc ctgaacccga
     3181 tgggcgcttc ggacgccatc gagttcctgc tcgagaagat gcgccagtcc aagaccaatg
     3241 gcgacttctt ccagtcgatg aacacctgac cggtcacggt agggccgaaa tgcaaagcgc
     3301 cgggggtaag accccggcgc tttttttctg gggcggaccg gtaaaagtcc tacggcagaa
     3361 gcagggtggc gccggtcgta cgccggccct ccagggcttc atgggcggcc cgggcttccg
     3421 agagcgggaa ggtctggccg atgtcgatct tcaccgcgcc tgatgcaagc acggcgaaca
     3481 gggcctgcgc gctctcgtcc agttcctcag tggtgacgat gtagtcgaac aggcgcgggc
     3541 gagtgacgaa cagcgacttg cccgacagct ccagcggtga gaacggcggg acgggaccag
     3601 aggcattgcc aaaggtcgcc agtacgccgc ggcgcgccag gctcttgagg ctggcttcaa
     3661 aggtgtcctt gccgaccccg tcatagacca cagctacgcc cttgccggcg gtaatctcgg
     3721 cgacgcgagc ggcgacgtct tcgtgcccat agaggatcac gtggtctgcg ccgagctcgc
     3781 gggcgatggc ggccttggcc accgacccca ccgtggcgat caccgtcgcg cccagggtct
     3841 tgcaccactg gaccaagatc gacccgacgc caccggccgc ggcgtggacc agcacgatgt
     3901 cgccgggttt cacgtgaaaa catcgccgga ccaggaactc ggcggtcatg cccttgagca
     3961 ggacggccgc ggcgacgtcg aggctgacgc tgtctggaac cttcaccgcg cgctcggccg
     4021 gcacgacggc cgcctccgca taggcgccaa gcgatccatt ataggcaaca cggtcgccag
     4081 cgacgaagcg cgtgacccct tcgccgaggg cctcgacaat gccggccgct tccagtccca
     4141 gcaccgcggg catcttcatc gggtagaggc cggagcggtg ataggtatcg atgaagttga
     4201 ggcccaccgc gtggtggcgg accaggattt gccccggccc cggcgcgggg acgggcaggt
     4261 caatggcctc gagcacctct ggaccgccgg cgcggacagc ttggatcgcc agcatcggcc
     4321 taggccaggt gggtcttgaa gaaggccagg cttcggccat tg
//
LOCUS       DAOMEO010000112         4350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2452_length_4350_cov_2.380210, whole genome shotgun sequence.
ACCESSION   DAOMEO010000112 DAOMEO010000000
VERSION     DAOMEO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_4350_cov_2.380210"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLH31_03615"
     CDS             <1..289
                     /locus_tag="PLH31_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88350.1"
                     /translation="AALSPSDVTATTLAGEPIIATLTSAPGNGEAIGGLKVVTQNAWF
                     AARPSGTEDVYKIYAESFLGDEHLKQVQDEAREVVAGALTGEGVSLSSSRA"
     gene            304..1074
                     /locus_tag="PLH31_03620"
     CDS             304..1074
                     /locus_tag="PLH31_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR88351.1"
                     /translation="MLTDPFFYAVAIPAVILLGLAKGGFAGIGVVAAPMLSLAVSPLL
                     AAAILLPILIVQDVVSVWAFRRTWDKGVLRLMLPAAAFGIFLGWALAAFVSAAAVELA
                     VGLISIVFALQRLWAERAVKAAEQVGQTAGRPWLGALCGLAAGFTSQIAHAGGPPFQI
                     YVLPRGLPRDTFIGTSAIFFAVVNWMKVPAYINLGQFTPGVLATAGVLLPLAIASTWT
                     GVWLVRRVPAEGFYRVIYVLLVAVGGKLVFDGVQGLVS"
     gene            1146..>1549
                     /locus_tag="PLH31_03625"
     CDS             1146..>1549
                     /locus_tag="PLH31_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQR88352.1"
                     /translation="MTDITTHYPTPAAKCADLVVHLAGLACALLGGGILLGLAFGMGD
                     LKQVAAVSVYTVGLILMLSLSTAYNFAKARWRPLLRRFDHAGIFIMIAASYTPFTTQN
                     LTGWWAIGMTTAVWSVATIGVLAKLFLPGLDKR"
     assembly_gap    1550..1559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1566..1766
                     /locus_tag="PLH31_03630"
                     /pseudo
     CDS             1566..1766
                     /locus_tag="PLH31_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518974.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1774..3006
                     /locus_tag="PLH31_03635"
     CDS             1774..3006
                     /locus_tag="PLH31_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR88353.1"
                     /translation="MSRGPDSKTTGQGLFGDWWTGDPLRGKRLATPPAEVLAEIVDFP
                     GVRLTADQADQALNAVADRLTPTRSLLDRTDFNILALSGGASGGAYGAGVLTGLSKAG
                     ARPEFAIVTGVSTGALIAPLAFLGSAWDERLTDSYVGGHAADLLSIKGFAGGLGAGLF
                     KGEGLEALVWPVIDEVVVEAVAREHARGRRLLIATTNLDNQKAVIWDMGAIASRGGEA
                     ALQLFRDVMLASASVPGLFPAKLFTVEAGGERYQEMHVDGGVAAPLFLMPDALLHWRS
                     LGQRLRRGRVYVIVNTVLDPSPRSTPAGVASIMVRGFETMLRFSYRQALSLAAGFCAR
                     HNLPLSVASIPTSIDYLNMLKFDTATMRAIFDEAERQAMAGNVWASPSPEPTGWRGLF
                     RRQPARADIDPGPTDVLE"
     gene            complement(3034..3354)
                     /locus_tag="PLH31_03640"
     CDS             complement(3034..3354)
                     /locus_tag="PLH31_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88354.1"
                     /translation="MSDSEPPRRKILSLKSGVAAPGGPKPLERMARPTRPIIPEPPPP
                     PPAAVGDWKCKPCGTRFDPSPDLADDDSVRCPSCNARLGLASDFRADPPNVEKLRARH
                     LKKA"
     gene            complement(3438..>4350)
                     /locus_tag="PLH31_03645"
     CDS             complement(3438..>4350)
                     /locus_tag="PLH31_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HQR88355.1"
                     /translation="GAPISASVAAPKTDTPAKTSPTSFKLDPVAAKPMSLDMRDARPV
                     DAAGFAPREVDSAQAKVEGAFALSPAATFGDARLLDSDRYYDDRGPVSWRSNAFVVGH
                     ASGAVDSVRVSVAAVARNAAVSPLSLFRPDAQAFDAENIDVTLTRGWPSAVKLEAGKY
                     ALDFSPHAGFGFGDAGGSAEAGATVRLGKNVEDRVSDALGLRDGDEAFGQRGRWYVFA
                     AASGRAVGLNMLRSQNGDWSRAGMSSDVNSKLIGDAQAGVAWRKGPMQASVGYIHREM
                     KAKDVIMGMATQKDSMVALSFSLKPNW"
BASE COUNT          624 a         1436 c         1496 g          784 t
ORIGIN      
        1 cgccgcgctg agcccgtccg atgtcaccgc caccactctg gccggtgagc cgatcatcgc
       61 gaccctgacc agcgctcctg gcaacggcga ggccatcggt ggcctgaagg tggtgaccca
      121 gaacgcctgg ttcgcggcgc ggccgtcagg caccgaggac gtctacaaga tctatgcaga
      181 gtcattcctg ggggatgagc atctgaagca ggtacaggat gaggcccggg aggtggtggc
      241 cggcgccttg acaggcgagg gggtgtccct gtcgtcatcg cgcgcctaga cctgccaggc
      301 tctatgctga ccgatccctt cttctacgcc gtcgccatcc cggccgtgat cctgctgggc
      361 ctggccaagg gtgggtttgc ggggatcggt gtggtggcgg cgcccatgct gtcgctggcg
      421 gtctcaccgc tgctggcggc ggccatcctc ctgccgatcc tgatcgttca ggacgtcgtt
      481 agcgtctggg cgtttcgccg gacttgggac aagggcgtgc tgaggctgat gctgccggcc
      541 gccgcgttcg gcatctttct gggctgggcc ctggcggcct tcgtgtcggc ggcggcggtg
      601 gagctggcgg tggggctgat ctcgatcgtg tttgccctgc agcgtctgtg ggccgagcgg
      661 gcggtcaagg ccgccgagca ggtggggcag acggccggtc gaccctggct cggcgcgctc
      721 tgcggcctcg cggccggctt caccagccag atcgcccatg ccggcggtcc gcccttccag
      781 atctatgtgc tgccgcgggg cctgccccgc gatacgttca tcggcaccag cgccatcttc
      841 ttcgccgtgg tcaactggat gaaggttccg gcctatatca acctgggcca gttcaccccc
      901 ggggtgctgg ccacggccgg cgtactgctg cctctcgcga tcgcctcgac ctggaccggc
      961 gtatggctgg tgcggcgcgt tcctgccgag ggcttttacc gcgtgatcta tgtccttttg
     1021 gtcgcggtcg gcggaaagct cgtctttgac ggcgttcagg gcctggtttc ctgacgaccg
     1081 gccgcccttt cggcgccatg atgatcgtcg ttcatacgtc actttctgag actagcgtgg
     1141 ccgccatgac cgatatcacg acccattacc cgactcctgc ggccaaatgc gctgacctcg
     1201 tggtccatct ggcaggtctg gcctgcgccc ttctcggcgg gggcatcctg ctgggcctgg
     1261 ctttcggcat gggcgacctg aagcaggtcg cggcggtttc ggtctacacg gttggcctga
     1321 tcctgatgct gtcgctgtcc accgcctaca acttcgccaa ggcgcgctgg cgtccgctcc
     1381 tgcgtcgttt tgaccatgcc ggcatcttca tcatgatcgc ggcgtcctat acccccttca
     1441 ccacccagaa cctgaccggc tggtgggcca tcggcatgac cacagcggtg tggagcgtgg
     1501 ccacgatcgg cgtgctggcc aagctgttcc tgccgggcct ggacaagcgn nnnnnnnnnc
     1561 cttgaggcag agcgcaaaac aggggaagat tttgcgcgat ggcattgtga tggggcggca
     1621 tcgtctattc gaccggcacg gtcttctacc tgatgcgccg cctgaagttc cgccgcgcca
     1681 tctggcatgg ccatgtggtc ggcggcgccg gcctgcacta tgccgccgtc ctggtgggcg
     1741 tggtcctggc gggttcgcac ggatgaggcg ggcttgagcc ggggacccga tagcaagacc
     1801 accgggcagg gcctgtttgg cgactggtgg acgggcgatc cgttgcgggg caagcgcctg
     1861 gcgaccccgc cagccgaggt gctagccgag atcgtcgact ttccgggtgt gcgcctgacg
     1921 gcggatcagg ccgatcaggc cctaaacgcc gtcgccgacc gcctgacccc gacccgttcg
     1981 ctgctggatc gcaccgattt caacatcctg gccctgtcag gcggcgcctc tggcggcgcc
     2041 tatggggccg gcgtcctgac gggactgtcc aaggcgggcg cgcgacctga atttgccatt
     2101 gtcaccgggg tcagcaccgg cgccctgatc gcgcctctgg cctttctggg ctcggcctgg
     2161 gacgagcgtc tgaccgactc ctatgttggc ggccacgcgg ccgatctgct cagcatcaag
     2221 ggtttcgccg gggggctggg ggccggactg ttcaagggcg agggtctgga agcgctggtc
     2281 tggccggtca ttgacgaggt ggtggtcgaa gccgtggccc gcgagcatgc ccggggccgg
     2341 cggctgctga tcgccaccac caatctcgac aaccagaagg ccgtcatctg ggacatgggc
     2401 gcgatcgcca gccgcggcgg cgaggccgcc ctgcagctgt ttcgcgacgt catgctggct
     2461 tcggcctcag tgccggggct gttcccggcc aagctgttca cggtcgaggc gggcggtgag
     2521 cgctatcagg aaatgcacgt cgatggcggc gtcgccgcgc cgttgttcct gatgcccgac
     2581 gccctgctgc actggcgcag tctgggccag cgcctgaggc gtggccgggt ctatgtgatc
     2641 gtcaacacgg tgctggaccc ttcgccgcgc tcgaccccgg ctggcgtggc ctcgatcatg
     2701 gtgcgcggct tcgagaccat gctgcggttt tcctatcggc aggcgctcag cctggcggcc
     2761 ggcttctgcg cgcgccacaa tctgccgctc agcgtcgcct cgatcccgac gagcattgac
     2821 tacctgaaca tgctgaaatt cgacaccgcg accatgcgcg ccatcttcga cgaggccgag
     2881 cgccaggcca tggcgggcaa tgtctgggcg agcccctcgc ccgagccgac cggttggcgg
     2941 ggtctgttcc ggcgtcagcc tgcccgggca gacatcgacc ccggcccaac cgacgttctc
     3001 gaatagctaa ccggctaaaa aaggcgggcg gccttaggcc ttcttcaggt gacgggcgcg
     3061 cagcttttcc acgttgggcg gatcggcccg gaaatcggac gccaggccca gacgcgcatt
     3121 gcaggacgga cagcgaacgg agtcgtcgtc ggccaggtcg ggcgagggat caaagcgcgt
     3181 gccacagggc ttgcacttcc agtctcccac ggccgcaggc ggcggggggg gcggctcagg
     3241 gatgatcggc cgcgtcggcc gagccatgcg ctcaagcggc ttggggcctc ccggcgcggc
     3301 gacgccgctc ttgaggctca ggatcttgcg gcgcgggggc tcgctatcgc tcatgacgtc
     3361 ggacagttcg tggacaggac cggcgctagc tgcatgatcg cggcgcgaaa ctcaagcgct
     3421 cgcgcgatat tgccggccta ccagttcggt ttcaggctga acgacagggc aaccattgag
     3481 tctttctggg tcgccatacc catgatgacg tccttggctt tcatctcgcg atggatgtag
     3541 ccaaccgagg cctgcatcgg acccttgcgc cacgccaccc cggcctgggc gtcaccgatc
     3601 agcttgctgt tgacgtccga gctcatgcca gcccggctcc agtcgccatt ctgactgcgc
     3661 agcatgttca ggcccacggc ccggccgctg gcggcggcga acacgtacca gcgcccgcgt
     3721 tgaccgaagg cctcgtcgcc gtcgcgcagg cccagggcgt cgctgacccg gtcctcgaca
     3781 ttcttgccca ggcgcaccgt ggcgccggcc tccgccgaac cgccggcgtc gccgaagcca
     3841 aagccggcat ggggcgagaa atccagggcg tacttgccgg cttccagctt gaccgccgaa
     3901 ggccagccgc gcgtgagggt gacatcgata ttctcggcgt caaacgcctg agcgtccggg
     3961 cggaaaaggc tcaatggcga cacggcggcg ttgcgcgcga cggcggcgac cgaaacccgc
     4021 accgagtcga cggcgccgct ggcatggcca accacaaagg cgttcgagcg ccagcttacc
     4081 ggcccgcggt cgtcataata acggtctgag tccagaaggc gcgcatcgcc gaacgtggcg
     4141 gcgggcgaaa gggcgaatgc gccttcgacc ttggcctgag ccgagtcgac ttcgcgcggc
     4201 gcgaagccgg cggcgtcaac cggacgcgcg tcgcgcatat ccaggctcat cggcttggcg
     4261 gcgacagggt caagtttgaa actggtcgga gaggtcttgg cgggcgtgtc agtcttggga
     4321 gcggcgacag aggcggatat cggcgcgccc
//
LOCUS       DAOMEO010000113         4325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2466_length_4325_cov_2.465808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000113 DAOMEO010000000
VERSION     DAOMEO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_4325_cov_2.465808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(799..1461)
                     /locus_tag="PLH31_03650"
     CDS             complement(799..1461)
                     /locus_tag="PLH31_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchor protein"
                     /protein_id="HQR88356.1"
                     /translation="MHNTQKPDLADLPTTSRLLRSTAFAVGIAATLLVTVVLPAEYAV
                     DPTGIGRLLGLTEMGSVKMAIAREAEQEESAEAAPAAAASGPVIAAHTAPTPVVTAVP
                     APAQAIAPAKQAPAAQAEEMTLTLAPGQGVEIKAKMRKGASVTYVWTTDGPKLNFDTH
                     GDGAGISYHGYGKGSESRSEGVLTAAFDGSHGWFWRNRAGKPVTVTLKVTGDFDGLKR
                     LV"
     gene            complement(1477..2124)
                     /locus_tag="PLH31_03655"
     CDS             complement(1477..2124)
                     /locus_tag="PLH31_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQR88357.1"
                     /translation="MTGLVSQAFAHGVAEGDKGYIQESSGVLFMPFVYLGAKHMITGY
                     DHLLFLFGVIFFLYRLKDVSLYVTMFAVGHSVTLLLGVLMGVNVSAYLIDAIIGLSVA
                     YKALDNLGAFQRWFGVQPNTKAATLIFGLFHGFGLATKLQDFQLSPDGLFVNLVAFNI
                     GVELGQILALSAILIAMGFWRRTSRFWTHAYTANVILMTAGFVLTGYQLVGYFVA"
     gene            complement(2414..>4325)
                     /locus_tag="PLH31_03660"
     CDS             complement(2414..>4325)
                     /locus_tag="PLH31_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQR88358.1"
                     /translation="ENCLRRFGEAQHRLGRLLNRDERFSLAASASSEVIRPSLFGVMI
                     ITLVYVPIFALTGVEGKMFHPMAITVVIALTAALVLSLTFVPAAVAMFVTGKVQEQES
                     FLMRWARIGYQPALDFAMRARTLMVVLAVALVAIAGFGASRMGSEFVPNLDEGDIAMH
                     ALRIPGTSLSQAIAMQTALEARISKFPEVERVVAKIGTAEVATDPVPPSVADTFIMLK
                     DRKDWPDPRKPRGKLVEEMEAAVNEVPGSKYEFTQPIQMRFNELLSGVRADVAIKIFG
                     DDLDELLKLGAEIEGIVGGVEGAADPQTEQITGLPVLQITPDRAALARLGLSIDDVQQ
                     VIATSMGGTTAGQIFEGDRRFDVVVRLPEDIRSNTDAIGRLQVPLPAGEGQPRQFVPL
                     SEVASIELTKGPNQISRENGKRRIVVTANVRDRDLGSFIAEVQQKVGAQVNVPAGYWV
                     TYGGTFEQLISAAKRLQLVVPAVLLLIFGLLFALFRSWRDSAVVFSGVPLALTGGVAA
                     LMMRGLPLSISAGVGFIALSGVAVLNGVVMVTFIRSLIAEGAPLDRAIREGALTRLRP
                     VLMTALVASLGFVPMALNVGAGAEVQRPLATVVIGGIISSTILTLLVLPALYSLVHTH
                     LGRRRAPAEALA"
BASE COUNT          830 a         1350 c         1418 g          727 t
ORIGIN      
        1 agcaggtgat ctaagccaat gagggtggga tcgcacatca gcaggtcgcc accgatcagg
       61 cgcatcacct cagcgtccgg tggcgggcga aggccgggac ggcgcccgcg aacgccccct
      121 ccattaccgc ttctccggac ggccgcgagg cgagctcctt aacggccgcg agggttgcct
      181 ggcacgtacg gccaagtcac ttagcgttcg gtccatcact ggctggaata tgcgtttaag
      241 gcggctcttc atcactgccg gtaggagtcc tctgaactta acgacgtggc agacgcgggt
      301 cgctccattc gcccgcgcaa accggtactg catctcgatg acgagcagcc cgggcatacc
      361 gagctgccag gcaagcccgc tgggagggtg gttggcggta aggcggccag ggaacaccca
      421 agatttctcc gggccgtcct tgaagatcac ctggatagca taggtgatcc ttgtccgatc
      481 atccggttct tcagtcacgc gagctgtacc agtccactta gggtaagacg cgaagtctgt
      541 caggatcgcc ctgactgtcg cgacaggcgc cttcacgtcg atctcgctca caagttcata
      601 cacgggagta ttccctttaa ctttcgccat ccgactattg cctgggcctt catccctcac
      661 cgctattgcc cagcagacgc ggacggcatt gtcgcgatgg tgcctaggta gccgatgatt
      721 gatcctgccg agacctagga ccagcttgca ggcgtctcgt acgcgcccac gcgccgaggt
      781 cggtttgcga tgaggcgctc acaccagccg cttcagaccg tcgaagtcgc cggttacctt
      841 cagggtcact gtgaccggct tgcccgcgcg gtttcgccag aaccagccat gggacccgtc
      901 gaaggcggcc gtgaggaccc cttctgagcg gctttctgaa cccttgccgt agccatggta
      961 ggagatgccg gctccgtcgc catgggtatc gaagttcagt ttcggaccgt cggtggtcca
     1021 gacataggtc acagatgcgc ccttgcgcat cttggccttt atctcaacgc cttgcccggg
     1081 ggccagggtc aaggtcatct cttcggcctg ggccgccgga gcctgcttag cgggggcgat
     1141 ggcttgagcc ggcgccggga cggccgtgac gactggagtg ggcgccgtgt gggccgcgat
     1201 caccggccca gaagccgcag ccgcaggtgc cgcctcggcg gactcctcct gttcagcctc
     1261 ccgggcgatg gccatcttca cactccccat ctcggtcagg ccgagcagcc ggccaattcc
     1321 ggtgggatcc acggcgtatt cggcgggaag cacgaccgtg accaggaggg tagcggcgat
     1381 acccacggcg aaggcggtgg agcgcagcag cctgctggtg gtgggcaggt cggcaaggtc
     1441 gggcttttgg gtgttgtgca tgggtctggt ctccggtcag gcgacgaagt agccgacgag
     1501 ttggtagccg gtgaggacga agccggcggt catgaggatg acgttggcgg tgtaggcgtg
     1561 ggtccagaac ctggaagtgc gccgccagaa tcccatggcg atcaggatcg cgctgagcgc
     1621 caggatctgg ccgagctcaa caccaatgtt gaaggcgacc aggttgacga aaaggccgtc
     1681 gggggaaagc tggaaatcct gcagcttggt agctaagcca aagccgtgga acagcccgaa
     1741 gatcagcgtg gcagcctttg tgttgggctg caccccgaac cagcgctgga aggcgccgag
     1801 attgtccagc gccttgtaag cgaccgagag gccaatgatg gcgtcgatca ggtaggcgct
     1861 gacattgacg cccatcagca cacccagaag cagggtgacc gagtgaccga ccgcgaacat
     1921 ggtcacatag aggctgacgt ccttgagccg gtagaggaag aagatcaccc cgaacaggaa
     1981 cagtaggtga tcgtagccgg tgatcatgtg tttggcaccg aggtagacga acggcatgaa
     2041 caggacgccg gagctctcct ggatgtagcc cttgtcgccc tcggcgacgc cgtgggcgaa
     2101 cgcctgagac accagaccag tcatcagaag cagaccgagg ccgagccaca aacgcttgga
     2161 ccaggttcgt agggttactt gtgtcatgga tgatccttgg aacttgaaga tcatgttgcg
     2221 acccatggat tggcgccgaa aagacgccga cggccccgga gctgggccgg tgggtcgaag
     2281 ggcgtgaggc gaaggcctat gcctcgccca gcatagtcgc ggcaacaagc gctgcgcctc
     2341 gcccagcgcc aggaaaaccc gacgctgggc gaggctggct tcgccgcagg cccactgagt
     2401 tcggggttcg aggtcatgcc aacgcctcgg caggcgcgcg cctacggcct aggtgagtgt
     2461 gcaccaggct gtagagggcc ggcaggacca gaagggtgag gatcgtcgag gagatgatcc
     2521 cgccgatcac gaccgtagca agaggtcgct gaacctcggc acccgctccg acattgagcg
     2581 ccatcgggac gaagccgagg ctggctacta gcgcggtcat cagcactggc cgaagccggg
     2641 tgagtgcgcc ttcgcggatc gcccggtcga ggggcgcgcc ttccgcaatc agactgcgga
     2701 tgaaggtcac catgaccacg ccattcagca ccgccacgcc cgagagcgcg atgaagccca
     2761 ccccggccga gatcgagagc ggcaggccac gcatcatcag cgccgccacc ccgccggtta
     2821 gcgccagggg cactccggag aagaccacag cgctgtccct ccaggagcgg aacagggcaa
     2881 acagcagacc gaagatcaac aagagcacgg ccggcacgac cagttggagg cgtttggccg
     2941 ccgagattag ctgttcgaag gtcccgccgt atgtcaccca atagccggcc gggacattca
     3001 cttgcgcgcc caccttctgc tgcacctcgg cgataaagga gccgaggtcg cgatcacgga
     3061 cgttggcggt caccacgata cggcgcttgc cgttctcgcg gctgatctgg ttgggccctt
     3121 tggtcagctc gatcgaggcg acctcactca gaggcacgaa ctgtcggggc tggccttcgc
     3181 ccgccggcag cggcacctgg agccggccga tggcgtctgt gttcgaccga atgtcctcgg
     3241 gcagccgcac caccacgtcg aagcggcggt caccctcgaa gatctgtccc gccgtggtcc
     3301 cgcccatcga ggtggcgatg acctgctgga catcgtcgat gctcaaacca agccgggcca
     3361 gggccgcacg gtcgggggtg atctgcagga ccggcagacc ggtgatctgc tcggtctgcg
     3421 gatcagcggc accttcgaca ccgccgacaa tcccctcgat ctcggccccg agctttagga
     3481 gttcgtccag gtcgtcgccg aagatcttga tggcgacgtc cgcgcgaacc cccgacaaca
     3541 gctcgttaaa ccgcatctgg atcggctggg tgaactcgta cttgctcccg ggcacctcgt
     3601 tgaccgcagc ttccatctcc tcgacgagct tgccccgcgg cttgcgcgga tccggccagt
     3661 ccttgcggtc cttcagcatg atgaaggtat cagcgactga cggcggcacc gggtcggtag
     3721 ccacctcggc ggtgccgatc ttcgcaacca cccgctcgac ttccgggaac ttggaaatac
     3781 gggcctccag cgctgtctgc atggcgatcg cctgactgag actggtgccg gggatgcgca
     3841 aggcgtgcat ggcgatgtcg ccctcgtcga ggttgggcac gaattccgac cccatgcgtg
     3901 aggcgccgaa tccggcgatc gcaacaaggg cgacggcgag aaccaccatc agggttcggg
     3961 ctcgcatagc gaagtcgagg gccggctggt agccgatccg cgcccagcgc atcaggaagc
     4021 tctcttgctc ctggaccttg ccagtgacga acatggcgac cgcggccggc acgaaggtca
     4081 gagacaggac gagcgcggcg gtgagcgcga tcaccacggt gatcgccatc ggatggaaca
     4141 tcttcccctc gacaccggtg agagcgaaga tcggcacata gactagggtg atgatcatca
     4201 ccccgaacag ggaggggcgg atcacctcgc tggaggccga ggccgccaga gagaagcgtt
     4261 cgtcacggtt cagaagccga cccaaacggt gttgggcctc gccaaatcgg cgaaggcagt
     4321 tttcg
//
LOCUS       DAOMEO010000114         4306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2479_length_4306_cov_2.827335, whole genome shotgun sequence.
ACCESSION   DAOMEO010000114 DAOMEO010000000
VERSION     DAOMEO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_4306_cov_2.827335"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLH31_03665"
     CDS             <1..566
                     /locus_tag="PLH31_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQR88359.1"
                     /translation="CGVNTIPTQDEATKTAWAEVQNQYQRRADLVPNLVATVKGYAAQ
                     EKDVLVAVTEARASATQVKVDASTITDPAAFEKYAAAQDQLSGVLGRLMMIQEQYPDL
                     KSNQNFMALQSQLEGTENRIAIARRDYNQTAQTYNTTLRTFPSILWAKTMYSGQKPAV
                     LFQATAAAQSAPTVNFDAAPPASAPAQ"
     gene            568..>1457
                     /locus_tag="PLH31_03670"
     CDS             568..>1457
                     /locus_tag="PLH31_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQR88360.1"
                     /translation="MKTRNLVTPEASERLSGAQELRTRPLGPGAWLRFGRDDRNWFVA
                     WLIAALAVACLAIPALAAPKFPPLSGRVVDEANILSPEVEADLTGKLKSLEDATGRQL
                     VVATVATLQGYEIEDYGYQLGRTWALGAKDKDDGAILLVAPAEKKVRIEVGYGLEPVL
                     TDALSSVIIQSAVIPKFKAGDLQAGVVAGTDELLTQLSLPDDEARANVAQAAAPEQRD
                     SGGFSPIAALLIGLFVFFLITRLLPGRRGRRSGLGSALPWIILNALTSGSGRGGGGWS
                     GGGGGGGFSGGGGSFGGGGSS"
     assembly_gap    1458..1467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1468..2166
                     /locus_tag="PLH31_03675"
     CDS             <1468..2166
                     /locus_tag="PLH31_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQR88361.1"
                     /translation="MKTMTPEDQARIAQAVAAAEKTTAGEIFCVLAPEVSDYRETPLV
                     WAAATALVLPAAGLLAGLRPDMLTQLFGGWTVGHASATDAAILSALSIYIGLQAAIFI
                     VSALFVSIPPVRRALTPRALKAAAVRRAAMEQFMSHGLHLTRDRTGVLIFAALAEHRV
                     EVIADEGIYKAAPNAVWDDVVADLVAGMKAGRIADGFVAAVTRVGEILSSHVPVRATD
                     TNELPDGLTILPKG"
     gene            2171..2983
                     /locus_tag="PLH31_03680"
     CDS             2171..2983
                     /locus_tag="PLH31_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQR88362.1"
                     /translation="MTGEASGRELRRVWFFLGLVFLISWGVGGLYLAFPAPLTAAFGP
                     FAYGSPAYLLAACSPTLVALGLTLTFEGPAGLARLGRRLLQATPLWALALAFLALPVI
                     ALGLGLLAPRFGVWPVRPVDVLVATPLILFTTAHILTNSGPLGEELGWRGYALPRLLN
                     RWPPLMAGGALGVIWTIWHIPAFLFSGIVATPLSDLGWYALGTVALSLLMTWLFLRTR
                     GSVLIAGVVPHFVINGLGAAGAWRARPAESVALALLVMALFLICPPNRRNHQ"
     gene            2996..3427
                     /locus_tag="PLH31_03685"
     CDS             2996..3427
                     /locus_tag="PLH31_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQR88363.1"
                     /translation="MSSIDPTREQFDAFKGLPRDTPIQMLNLVRLKAVADYPVDHPEH
                     GKGRSGLDAYRAYGRETAALFARLGGRQIWAGKPELVLTGPTDERWDLAFIAEYPNAG
                     AFFAMVTDPAYREFVKHRTAAVEDSRLIRLSPLTPGEGFGE"
     gene            complement(3484..>4306)
                     /locus_tag="PLH31_03690"
     CDS             complement(3484..>4306)
                     /locus_tag="PLH31_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88364.1"
                     /translation="QAAQGFRLGGVNDPLNLPLCTPQDAAIFGGFQSYDDETLWNYEA
                     GVKSRFGGISFNSAVFYTDIKNLQTTLDAGSCSSRVVFNVPKAHTKGIEAELSARLAP
                     GFDIGISGSLLEAEFDSTVKDGTGAVLGGIREGNRLPSVPKFQISADATYSWTVRSGM
                     EAYVSASVQHIGSRYTQASDQENNPRSFVSGLPFGGATGTVPTVVNLQLPSYEIVNFS
                     IGLEMDNGVDIIAYANNVFDENPLLSFDRERGGRARLGYTVGQPRTMGVTVRRSF"
BASE COUNT          645 a         1513 c         1425 g          713 t
ORIGIN      
        1 gctgcggggt caacaccatc cccacccagg acgaggcgac caagaccgcc tgggccgaag
       61 tgcagaacca gtatcagcgc cgcgccgacc tggtgcccaa cctcgtcgct acggtgaagg
      121 gctacgccgc ccaggaaaag gacgtgctgg tcgccgttac ggaagcccgc gccagcgcca
      181 cccaggtgaa agtcgacgcc tcgaccatca ccgacccggc cgccttcgag aaatacgccg
      241 ccgcccagga ccagctgtcg ggcgtgttgg gccggctgat gatgatccag gagcagtatc
      301 cggacctgaa gtcgaaccag aacttcatgg ccctgcaaag ccagctggaa ggcacggaaa
      361 accgtatcgc catcgcccgc cgcgactaca accagacggc ccagacctac aatacgacgc
      421 tgcggacctt ccccagcatc ctctgggcca agacgatgta ttcgggccag aagccggccg
      481 tgctgttcca ggccacggcg gcggcgcaat cggccccgac cgtgaacttc gacgcggccc
      541 cgccggcctc ggcgccggct cagtaagttg aagaccagaa accttgtcac cccggaagcc
      601 tcggagaggc tatccggggc ccaggagctg cgcacgcggc ccctgggtcc cggcgcttgg
      661 ctgcgcttcg gccgggatga caggaattgg tttgtcgctt ggctgatcgc cgctttggcg
      721 gtggcctgcc tggccatccc cgccctcgcc gcccccaagt tcccgcccct gtccggccgg
      781 gtggtcgacg aggccaacat cctctcgccc gaggtcgaag ccgacctgac cggcaagctg
      841 aagtcgctgg aagacgccac cggccgccag ctggtggtgg ccacggtcgc caccctgcag
      901 ggctacgaga tcgaggacta cggctaccag ctgggccgca cctgggccct gggcgccaag
      961 gacaaggacg acggcgcgat cctgctggtc gcgcccgccg agaagaaggt gcgcatcgag
     1021 gtcggctatg gcctggagcc cgttctgacc gacgccctgt ccagcgtcat catccagagc
     1081 gccgtcatcc cgaaattcaa ggccggcgac ctgcaggccg gcgtcgtggc cgggaccgac
     1141 gaactgctga cccagctgtc cctgcccgat gacgaggcca gggccaatgt cgcccaggcc
     1201 gccgcgccgg agcagcgtga cagcggcggc ttctcgccga tcgccgccct gctgatcggg
     1261 ctgttcgtgt tcttcctgat cacccgcctg ttgcccggcc gacgcggacg acgcagcggt
     1321 ctgggctcgg ccctgccgtg gatcatcctc aacgccctga ccagcggttc gggtcgcggt
     1381 ggtggcggct ggagcggcgg cggaggcggc ggagggttct ccggcggcgg cggctcgttc
     1441 ggcggcggcg gaagttcnnn nnnnnnnatg aagacgatga ctcccgaaga tcaggcccgc
     1501 atcgcgcagg cggtcgccgc ggccgagaag accaccgccg gcgaaatctt ctgcgtcctg
     1561 gcgcccgagg tgtccgacta tcgcgagacg cccctggtct gggcggcggc gacagccctg
     1621 gtcctgcccg ccgccggtct gctggcagga ctgcgccccg acatgctcac ccagctgttt
     1681 ggcggctgga ccgtgggcca cgcctcggcc accgacgcgg cgatcctctc ggcgctgtcg
     1741 atctatatcg gcctgcaagc ggcgatcttc atcgtctccg ccctgttcgt ctcgatcccg
     1801 cccgtccgcc gcgccctgac gcctcgggct ctgaaggcgg cggcggtcag gcgggcggcc
     1861 atggagcagt tcatgagtca cggtctgcac ctgacccgcg accgtaccgg cgtgctgatc
     1921 tttgccgccc tggccgagca ccgcgtcgag gtcatcgccg acgagggcat ctacaaggcc
     1981 gcccccaatg cggtgtggga tgacgtggtc gccgatctgg tcgccggcat gaaggctggc
     2041 cgcatcgccg acggcttcgt cgccgccgtt acccgggtgg gtgagattct ctccagccac
     2101 gtgcccgtcc gcgccacaga caccaacgaa ctgcccgacg gcctgaccat tctgcccaag
     2161 ggctgatccg atgacggggg aagccagcgg ccgggaactc cgacgcgtct ggttcttcct
     2221 cggcctcgtc ttcctgataa gctggggtgt tggcggactg tatctggcct ttcccgcgcc
     2281 gttgaccgcc gccttcggtc ccttcgccta tgggtcgccg gcctatcttc tggccgcctg
     2341 ttcaccaacc ctggttgctc tggggctgac cctgaccttc gaaggaccgg cgggcctcgc
     2401 gcgccttggc cgccgattgc tccaggcgac gccgctgtgg gccttggccc tggcgttcct
     2461 ggccctgccc gtcatcgccc tgggcctcgg cctgctggcg ccccggttcg gcgtctggcc
     2521 ggtgcgcccc gtcgacgtgc tggtcgccac accgcttatc ctgttcacca ccgcccatat
     2581 cctcaccaat tccggtccgc taggcgagga gctgggctgg cgcggctatg ccctgccccg
     2641 gctgctgaac cgctggccgc ccctgatggc cggcggcgca ttgggggtca tctggacgat
     2701 ctggcacatc ccggccttcc tgttttcggg catcgtcgcc acgccgctgt cagacctggg
     2761 ctggtacgct ctgggcacgg tcgccctctc gctgctgatg acctggctgt tcctgcggac
     2821 gcgcggcagc gtgctgatcg ccggggtagt tccgcacttc gtgatcaatg gcctgggcgc
     2881 ggcgggggcc tggcgcgcgc gaccggccga aagcgtcgcc ttggccctgc tggtcatggc
     2941 gcttttcctg atttgcccgc caaatagacg caatcatcaa taggaggagc gcgccatgtc
     3001 cagcatcgac ccaacccgcg agcagttcga cgccttcaag ggccttccgc gcgatacgcc
     3061 gatccagatg ctgaacctgg tgcgcctgaa ggccgtcgcc gactatccgg ttgatcaccc
     3121 cgagcacggc aagggccggt cgggcctgga cgcctatcgc gcgtatggcc gcgagaccgc
     3181 ggcgctcttc gccaggctgg gcggccggca gatctgggcg ggcaagccgg aactggtgct
     3241 gaccggcccg accgatgagc gctgggacct ggcctttatc gccgagtatc ccaatgccgg
     3301 cgccttcttc gccatggtca ccgacccggc ctatcgcgag ttcgtcaagc accggacggc
     3361 ggcggtcgag gactcgcgtc tgatccggct gtcgcccctg acgcctggcg aaggcttcgg
     3421 cgaatagaaa cggggcggcc gcttgcgcga cctccccgcc gatcccaacc tcgagaggcg
     3481 ggactagaag ctccggcgca ccgtcacgcc catcgtgcgc ggctggccga ccgtgtagcc
     3541 caggcgcgcc cgaccaccgc gttcgcgatc gaacgacagc agcgggttct cgtcgaacac
     3601 gttgttggcg taggcgatga tgtcgacgcc gttgtccatc tccaggccga tgctgaaatt
     3661 gacgatctcg tagctgggca gctggagatt gacgaccgtc ggcacggttc cggtggcccc
     3721 accgaacggc aggccggaga cgaaggaacg cggattgttc tcctggtcgc tggcttgggt
     3781 gtagcggctg ccgatatgct gcaccgaggc gctcacatag gcttccatcc ccgagcggac
     3841 ggtccacgaa taggtggcgt cggccgagat ctggaacttg ggcaccgacg gaaggcggtt
     3901 gccctcacgg atgccgccca gcaccgcgcc cgtcccgtcc ttcacggtgg agtcgaactc
     3961 ggcttccagc aggctgcccg agatgccgat gtcgaagccg ggcgccaggc gcgcagagag
     4021 ctcggcctcg atgcccttgg tgtgggcctt gggcacgttg aacaccacgc gcgaggagca
     4081 cgagccggcg tccagcgtgg tctgcaggtt cttgatatcg gtatagaaga cggcgctgtt
     4141 gaagctgatc ccgccgaagc gcgacttcac gccggcctcg tagttccaca gggtctcgtc
     4201 gtcatacgac tggaagccgc cgaagatcgc cgcgtcctgc ggggtgcaga gcggcaggtt
     4261 cagcgggtcg ttgacgccgc ccagccggaa gccctgggcc gcctgg
//
LOCUS       DAOMEO010000115         4292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2491_length_4292_cov_2.554166, whole genome shotgun sequence.
ACCESSION   DAOMEO010000115 DAOMEO010000000
VERSION     DAOMEO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_4292_cov_2.554166"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="PLH31_03695"
     CDS             <1..2021
                     /locus_tag="PLH31_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR88365.1"
                     /translation="AKTTAFDLQLDPSSPGLKFHRIDKSRDPNFWSIRVSQDIRIIVH
                     KTEASFLLAYVDHHDRAYAWAERRRIEAHPRTGAIQIVETRELAEDLPAPAPVSMLPT
                     ATPSPVVRPPLFSALSDEDLLGIGAPVDWLSDIHAATEDTFFDLAERLPAEVAEALLD
                     YVSAGVLKAPQPAAPAADPFTHPDTLRRVRTVDSEDALRLALDYPWDRWSIFLHPSQA
                     AVVERVFNGPARVTGTAGTGKTVVALHRAARAVSDDPAARVLLTTFSRPLANALRPKL
                     DLLLLGQPEALRRVTVASFEDIASELFQLAFARKPAIASDAVLDAILRKEAEAVGTED
                     PGGRFLSAEWRYVVDAWASEGPEAYATVPRIGRRNRLGARQRERLWPAFEAARRSLAA
                     RGLMTWPQVFYAVRAYFAERPDKPFTHVIVDEAQDLGVPQLRMLAAVSEGSGLFFAGD
                     LGQRIFQQPFSWKALGIDLRGRSSALKVNYRTSRQIREAADRLLPPEVRDADGLEDSR
                     RGAMSVFEGPAPIILVTANQEDEIAAVAAFLRAAVDDGVAPGEISIFVRAMDQLPRAR
                     AAIAAADMVCIQLTERAEEAADRVSIGTMHLAKGLEFKAVAVMACDDDVLPLQSRIDG
                     ATDEDELREVYDTERHLFYVACTRARDRLQISGVRPGSEFIGDFASEP"
     gene            complement(2133..>2578)
                     /locus_tag="PLH31_03700"
     CDS             complement(2133..>2578)
                     /locus_tag="PLH31_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88366.1"
                     /translation="ASTPSRYHVDASKIFERSAKEGRKYGMFLVVASQRPSELSKTVL
                     SQCSNFVVHRIQNPDDLSQIRQMTPFISDAVLKRLPSLPKQHALVFGTSVNLPTTFKV
                     RNADPRPSSDDTRIVDLWFHEEGRLADVALLSAAPTARDDVEDLV"
     assembly_gap    2579..2588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2589..4166)
                     /locus_tag="PLH31_03705"
     CDS             complement(<2589..4166)
                     /locus_tag="PLH31_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HQR88367.1"
                     /translation="MSHDDAKRAVGKVTSVAADRFVIEMHAGTDNFTVVGFDDVHYVA
                     RLGSFLMIPTQSEYVVVEVVGLRERDASSSTRGPGELDKTGSAKFLDVVPVGMLPSPG
                     AGKFRFGVSVFPSLYADALYALDSELDRIFETDEAIEPSVGPPGGPCVPTGATRYRAL
                     EIGQSVVFDHYKVKVRLDDFFGGHVAVLGNTGSGKSCTVASVLQSLFEKPSEHHARGA
                     TFVVFDVNGEYDAALSKLGEKPGIGVSKVVLDGSADKTRFRLPHWFLDTAEWELLLQA
                     SEKTQIPVLRTALGLTGLRSRNAAEQRELQEHFVATCVIECFRGADGDSPVSKIQRVW
                     TLLDKYGTPNLNRSILTKHRVNEKFGNFESSGSQTAFLDEMKTKLREDVKLPSFDNQP
                     FSFNDLEECLDFALLYEEAHGNRQIRDYCSQMVTRLKSLKDRRDYAFLRYDLKVDTAP
                     PSREAFLANLLGLAVKDKKYVKSSQIIILDMNEVDDEVVELVSAVVARMSFSILRRAD
                     PRNRFPVHLLLEEAHRYV"
BASE COUNT          772 a         1381 c         1339 g          790 t
ORIGIN      
        1 cggcgaaaac tacggcgttc gacctccaac tcgacccgtc ttcgccgggc ctgaaattcc
       61 atcgcatcga taagtcacgc gacccgaatt tttggtccat ccgcgtcagc caggacatcc
      121 gcataatcgt ccacaagact gaggccagtt tcctgctggc ctacgttgac caccacgacc
      181 gggcctatgc ttgggcggaa cggcgacgga tcgaggcgca tccgcgcacc ggcgccatcc
      241 agatcgtcga gacccgtgaa ctggcagaag atctgccggc gccggcacca gtgtcgatgc
      301 tcccgacggc aacgccctct ccggtcgtcc gccctcccct gttctccgcc ttgtcggacg
      361 aagacctgct cggcataggc gcgccggtcg actggctaag cgacattcac gcggccactg
      421 aggacacatt cttcgatctc gccgaacgcc tgccggccga ggtggcggag gcattgctag
      481 attatgtgtc cgccggcgtc ctgaaggcgc cccagcccgc cgcgcccgcc gctgatccgt
      541 tcacccaccc cgacaccctt cgccgcgtgc gcaccgtgga cagcgaagat gccctgcggc
      601 tcgccctcga ctacccatgg gaccgctgga gcatcttcct gcacccctca caggccgctg
      661 tggtcgagcg agtgttcaac ggtccggcgc gtgtcaccgg cacggccggc accggcaaga
      721 ccgtggtcgc cttacaccgc gcagcgcggg cggtatccga cgatccggcg gcgcgcgttt
      781 tgctgacgac gttctcgcgc ccactcgcca acgcactacg tcccaaactc gacctgctgc
      841 tgctcggcca gcccgaagcc ctgcgccggg tgacggtcgc ctccttcgag gacatcgctt
      901 cggaactgtt tcagctggct ttcgcgcgca agccagcgat cgcgtctgac gcggtgctgg
      961 acgcgattct ccgcaaggag gccgaagccg tgggcaccga ggacccgggc ggacgcttcc
     1021 tgtcggctga atggcgctat gtggtggacg cctgggcgag cgaggggcct gaagcctatg
     1081 ccaccgtccc ccgtatcggc cgccgcaacc ggctgggggc acgccagcgg gagcggcttt
     1141 ggccagcgtt cgaagcagcg cggcgctcac tcgctgcgcg cggcctgatg acctggcctc
     1201 aggtgtttta tgctgtgcgg gcctatttcg ccgagcggcc ggacaaaccc ttcacccatg
     1261 tgatcgtcga tgaggcccaa gatctcggcg tgccacagct aaggatgtta gccgctgtca
     1321 gcgagggttc gggcctcttc ttcgccggcg acctcggcca gcgcatcttc cagcagccct
     1381 tctcgtggaa ggcgctgggc atcgacctcc ggggccgatc gtcggcgttg aaggtcaact
     1441 accggacctc tcgtcaaatc cgcgaagccg ccgaccggct gctgccgccc gaggtccgcg
     1501 acgccgacgg acttgaggac agtcgccgcg gcgccatgtc ggtgttcgag gggccggcac
     1561 ccatcatttt ggtcacggcc aaccaggagg atgagatcgc cgccgtcgcg gccttcctgc
     1621 gcgcggcggt cgatgacggc gtcgcgccgg gggagatcag catcttcgtc cgggccatgg
     1681 accaactgcc ccgcgcccgc gctgctatcg cggcagcgga catggtctgc atccagttga
     1741 ccgagcgcgc cgaggaagcc gccgaccgcg tttccatcgg caccatgcat ctcgccaagg
     1801 ggcttgagtt caaggccgtc gcggtcatgg cctgcgacga cgacgttctg ccgctccagt
     1861 cgcgcatcga cggcgccacg gatgaagacg agctccgcga agtctatgac accgaacggc
     1921 acttgttcta cgtcgcctgt acccgcgcga gggacaggct tcagatcagt ggcgtgcggc
     1981 ccggatcgga attcatcggt gattttgcgt ccgaaccgta gaactcgact agcaccggtc
     2041 tggaacccaa agttgggttg aatcgcctga aacaggctcc aggccccagt gtcgcgataa
     2101 gctgtcttag gcgcgaaacg cgtgtatccc gctcacacca agtcctcaac gtcatccctg
     2161 gctgttggag cggccgatag cagcgccacg tccgcaagcc ggccttcctc gtgaaaccag
     2221 agatcgacga tacgtgtgtc gtcgctcgat gggcgaggat ccgcgttcct gaccttgaaa
     2281 gtcgtcggca agttcaccga cgtgccgaac accaaggcat gctgtttcgg caacgacggt
     2341 aggcgcttaa gcacagcgtc ggagatgaag ggcgtcatct ggcggatttg cgaaaggtcg
     2401 tcgggatttt gaatcctatg gacgacgaag ttggagcatt gggacaggac ggtctttgaa
     2461 agttcgctcg gccgctggga cgccacgacg aggaacattc cgtatttgcg gccctctttg
     2521 gcgctccgct cgaatatctt cgatgcatcg acgtggtagc gggagggggt cgaggcgann
     2581 nnnnnnnnga catagcggtg agcctcctcc agcagcaggt gcactggaaa gcgattgcga
     2641 ggatcggcgc ggcgcagaat agaaaacgac atccgcgcca ctaccgcgga gacaagctcg
     2701 accacctcat cgtcaacttc gttcatgtcg agaatgatga tctggctcga cttgacgtat
     2761 ttcttgtcct ttaccgccag gcccaggagg ttcgccagga acgcctcgcg actgggcgga
     2821 gccgtatcga ccttcaggtc gtacctcaaa aaggcgtagt cgcgacggtc cttaaggctc
     2881 ttcagccggg tgaccatctg cgagcaatag tcgcggattt ggcggtttcc gtgcgcctct
     2941 tcatagagca gcgcgaagtc gagacattcc tccaagtcat tgaagctgaa gggctgattg
     3001 tcgaaggagg ggagcttcac gtcctcgcga agcttggtct tcatttcgtc caggaaggcc
     3061 gtttggctgc ctgacgactc gaagttgccg aacttttcgt tgacccgatg tttggtcaaa
     3121 atggaccggt tcaaattcgg cgtgccatac ttgtccagca aggtccaaac gcgctggatc
     3181 ttcgacaccg gggagtcgcc gtcggcgccg cggaagcact cgatcacaca ggtcgcgacg
     3241 aaatgctcct gcagttcgcg ctgttcggcg gcgttgcggg accgcaagcc ggtcagtccc
     3301 agcgctgtcc taagcacggg gatctgagtt ttttcactgg cctgcagcag aagctcccac
     3361 tccgcagtgt cgaggaacca gtgaggcaac cgaaacctag ttttgtcggc cgacccgtcc
     3421 aggacgacct tggagacccc gatcccaggc ttctcaccca acttgctcaa tgccgcgtcg
     3481 tactcgccgt tgacgtcaaa aacgacgaac gtggcgccgc gtgcgtggtg ttcggacggc
     3541 ttttcgaaga gtgactgaag gactgaggcg acggtgcatg atttgccgct gccggtgttg
     3601 ccgagcactg cgacatgacc gccgaagaaa tcgtcgagac gcaccttcac cttgtagtgg
     3661 tcgaacacca ccgactggcc gatctcgagc gctcggtagc gcgtggcgcc cgttggaacg
     3721 cacggcccgc ccggcgggcc gacgctgggc tcgatcgcct catcggtctc gaaaatgcga
     3781 tctagctcgc tgtcgagggc gtagagggcg tccgcgtaga gcgagggaaa gacggagacg
     3841 ccgaaacgaa acttgcccgc gccgggcgac ggcaacatcc cgacaggcac cacgtcgagg
     3901 aacttcgcgg accctgtctt atcaagttcg cccgggccgc gcgtcgatga gctggcgtcg
     3961 cgttcgcgaa gcccgaccac ttcgacgaca acatattccg actgggtggg gatcatcagg
     4021 aaggagccca gccgcgccac gtagtggacg tcgtcgaacc cgaccacggt gaaattgtcg
     4081 gttccggcgt gcatctcgat gacgaaccgg tcggcggcga ccgatgtcac cttgcccacc
     4141 gcccgtttcg cgtcgtcgtg gctcatgcgt cggcatcccg atccggcgcc atggtttgga
     4201 tgaccttgcg aaccgcttcg tcgatgcgct cgctggagcg ctgaggcatg aacttgtcga
     4261 ccacggtggc gaagtagtgg gcgggggtct cc
//
LOCUS       DAOMEO010000116         4270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2501_length_4270_cov_3.029656, whole genome shotgun sequence.
ACCESSION   DAOMEO010000116 DAOMEO010000000
VERSION     DAOMEO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_4270_cov_3.029656"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLH31_03710"
     CDS             <1..638
                     /locus_tag="PLH31_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQR88368.1"
                     /translation="YGITAHLICQSFNDVFSKYGDKTPIFDNMHITATFATSEPTSID
                     KVIRRAGKALEMRESYSDPRSIFGSSHRSTSQSEHERYILTEDRVRELDDNQQFLFVN
                     NTKPIRAEKIRYYDEPFFKARTGDYFHGVPAKYEQRPGAADLPGPAQIDWLGVRAAEP
                     APDGLKGVVPPSAPAAADEGPQSAGDGGHGLHAPVSSLRWTGDDDDDGSLG"
     gene            690..1652
                     /gene="trbB"
                     /locus_tag="PLH31_03715"
     CDS             690..1652
                     /gene="trbB"
                     /locus_tag="PLH31_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HQR88369.1"
                     /translation="MPQHHPVVTDRKVAALKQAMGPVIASALADRMVVEVMVNPDGKI
                     WVDRIGEGRSFTGQSLASADADRILRLLADHVGEVVTRDRPRVSATLPETGERFQGAF
                     MPIVSSPAFAIRKRPEVVFTLPEYVDQGIMTEHQAQVIREAATARQNILIVGGTGSGK
                     TTLANAILAEPAFAQDRVVLIEDTAELQCSAEDKIQMLTKRTDPPVTMTDLVRDTLRL
                     RPDRIIIGEVRDGSALDLLKAWNTGHPGGLATIHANSAAEGLTRLEDLIGEVTQRIPY
                     RAITQAINLVIHIARAPAGRRVQEVCRVTGREGDAYVLEACIEG"
     gene            complement(1980..2471)
                     /locus_tag="PLH31_03720"
     CDS             complement(1980..2471)
                     /locus_tag="PLH31_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88370.1"
                     /translation="MAVLVSDTSVIIDLERGTLLDDLFRLPFEFAVPDLLFRRELAGA
                     LGDRLIALGLRVEELTSVELTRATTVRRERKELSTPDTFAFAIAEGRQWPLLTGDGGL
                     RRLAAECQVAFHGVLWICDQLETGSHVPRPRLHAGLTALSSHPRCRLPAAEVRQRLDR
                     YAE"
     gene            complement(2476..3540)
                     /locus_tag="PLH31_03725"
     CDS             complement(2476..3540)
                     /locus_tag="PLH31_03725"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQR88371.1"
                     /translation="MIGHKIKVARAAAGLSLRGLAEVMGGVVSAQAIGKYERNEDMPG
                     SRALMALADSLGVTEDYLLSEDEIALEGVDFRKKAGSSAREEATLEARTIHMLERYLT
                     IEDLLHLRSVDWEQPRSAPHHVGDLRDAEDAARSVRDDWGLGNDPIPQLAELLEERGV
                     KVMSLDLDDIDGLAAKVRRRGRDAARVIVIKRSSWSERKRFNLAHELGHMVLSVAAGV
                     NEEKAAHRFAGAFLVPADVLRAEVGASRSSLSLGELVALKERFGVSLQALTYRCKDLG
                     IINQAAFAKLFRVFAERGWRAYPFEEPGRMKPELEEPRRFERLCYRALAEGVIGEARA
                     AELLGISSRELDARLDQVAA"
     assembly_gap    3857..3866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3867..>4270
                     /locus_tag="PLH31_03730"
     CDS             <3867..>4270
                     /locus_tag="PLH31_03730"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88372.1"
                     /translation="ITKVVKAGSFAKYTILRKTAVDPVDVDVVFYISGRDINHETLKS
                     INDQIYDLLIKIYPTKSVEDFEIQRKAATVTFVGSGLSVDIVPVIEDPNQAGYGWQFD
                     IHDGSKVQTCAPCQIKFVRDRKDQDKDFRTLV"
BASE COUNT          785 a         1378 c         1370 g          727 t
ORIGIN      
        1 gctacggcat caccgcccac ctgatctgcc agagcttcaa cgacgtgttc tccaagtacg
       61 gggacaagac gccgatcttc gacaacatgc acatcaccgc caccttcgcg acctcagagc
      121 cgaccagcat cgacaaggtg atccggcggg ccggcaaggc gctggagatg cgggagagct
      181 acagcgatcc gcgcagcatc ttcggcagtt cgcaccgctc gacctcccag agcgagcacg
      241 agcgctacat cctgaccgag gaccgggtcc gcgagctgga cgacaaccag cagttcctat
      301 tcgtcaacaa caccaagccg atccgggcgg aaaagatccg ctactacgac gagccgttct
      361 tcaaggcgcg aacgggggac tatttccacg gcgtgcccgc caaatacgag cagaggccgg
      421 gcgcggccga cctgcccgga cccgcgcaga tcgactggct tggggttcgc gccgcagagc
      481 cggccccgga cggcctgaag ggtgtcgtgc cgccgtcggc ccccgcggcg gcggacgaag
      541 ggcctcagtc cgccggcgac ggcgggcacg gcctccacgc gccggtctcg agcctgcgat
      601 ggactggcga cgatgatgac gacggcagcc tgggctgacg aacctgccga gacgcccacg
      661 cccatgactc cagttgaatt ggatttccga tgccgcagca ccaccccgtc gtcaccgatc
      721 gcaaggtcgc cgcgctgaag caggccatgg gtccggtgat cgccagtgcg ctcgccgacc
      781 gcatggtggt ggaggtcatg gtcaatccgg acggcaagat ctgggtggac cggatcgggg
      841 aggggaggtc gttcaccggc cagagcctgg ccagcgccga cgccgatcgg attctacggc
      901 tactggccga ccacgtgggc gaagtggtca cccgggaccg gcctcgggtc agcgccacgc
      961 ttccggagac tggagagcgc ttccagggcg ccttcatgcc gatcgtttcg agccccgcct
     1021 tcgcgatccg caagcggccg gaggtggtgt tcactctgcc cgaatacgtc gaccagggga
     1081 tcatgaccga gcaccaggcg caggtgatcc gcgaggcggc gacagcgcgt cagaacatcc
     1141 tgatcgtggg cggcacgggc tcgggcaaga cgacgctcgc caacgccatc ctggccgagc
     1201 cggcctttgc ccaggaccgc gtggtgctga ttgaggacac cgccgagctt cagtgctcgg
     1261 cggaggacaa gatccagatg ctcaccaagc ggacggaccc gccggtgacc atgaccgatc
     1321 ttgtccgcga caccctgcgc ctgcggccgg accgcatcat catcggggag gtgcgggacg
     1381 gatcggcgct ggatctcttg aaggcgtgga atacgggcca ccctggcggg ttggcgacta
     1441 ttcacgccaa cagcgctgcg gagggcctga cacggttgga ggacctgatc ggggaggtga
     1501 cccagcggat tccctatcgg gcgatcaccc aggcgatcaa cctggtgatc catatcgccc
     1561 gcgcgccggc cggacggcgg gtccaggagg tctgccgggt gaccggccgc gagggtgatg
     1621 cctatgtcct ggaggcctgc atcgagggtt aggcggtcag gtgtgacgaa tttcgtgttg
     1681 tggcattcgg tctgggtggt gaggcgaggg taatatgggg aaggtttccc ctgcggccga
     1741 gccaggtctc tgccgacccc ttcgagcggg gacaccccgc aacgccccgg attcggagag
     1801 cggccggcgg gcgtgctcgg tcccgccgct gcgctgctcc tgcgggtccg cccctggcgg
     1861 gtgcctttgg ggcaccgccg gccactcgac ggcgcgttcc gccacgcgcg atgcgcgggc
     1921 tccctgcagc cttcatttta tttaggcgcg gtctcgtccg gatcagacgg gttgcgaccc
     1981 tattcagcgt atcgatcgag gcgctggcgc acctcagcgg ctggcagccg gcaacgggga
     2041 tgagacgaga gtgcggtcag gccggcgtgt agccttggcc gcggcacatg cgatcccgtt
     2101 tcgagctggt cgcaaatcca aagaacgcca tgaaaggcca cctggcactc cgccgcgaga
     2161 cgccgaaggc cgccatcgcc tgttagcaag ggccactgtc gaccctctgc gatggcgaac
     2221 gcgaaggtgt cgggcgtgga gagctccttc ctttctcgac gaaccgtcgt ggcgcgcgtc
     2281 aattcgaccg aagtcagctc ctcgacccgc aatccgagcg caatgagccg atcgcctagg
     2341 gcgccggcga gttcacggcg gaataggaga tcgggcaccg caaattcaaa ggggaggcga
     2401 aagaggtcgt ccagcagcgt tccccgctcc aggtcgatga tgaccgaggt gtcggaaacc
     2461 aggaccgcca ttggactatg ccgccacctg atcgagacgg gcgtccagtt cgcgcgatga
     2521 gatgcctagg agctcggctg cgcgcgcttc accaatgacc ccttcggcca gtgcgcgata
     2581 gcacaggcgc tcgaaccgtc tgggctcctc tagctccggc ttcatcctgc cgggctcttc
     2641 gaatgggtag gccctccagc ctcgttccgc gaagacgcga aacagcttcg caaaggccgc
     2701 ctggttgatg atgcccaaat ctttacaccg ataggtcagg gcctgcaggc tgacgccaaa
     2761 ccgttccttg agcgcgacga gttcgcctaa gcttagcgac gatcggctgg cgccgacctc
     2821 tgcgcgcagc acgtctgccg gcactagaaa cgcacccgcg aatctgtgcg cggccttctc
     2881 ctcattgacg ccagcggcca cactcaggac catgtggcca agctcgtgtg cgaggttgaa
     2941 gcgtttcctc tccgaccaac tgctgcgttt tatgacgatg acgcgggccg cgtctcggcc
     3001 tcgccgccgg accttggccg ccaggccgtc gatgtcatcg aggtcaagtg acatcacctt
     3061 cacgccgcgt tcctcaagaa gttccgcgag ctgagggata ggatcattgc ccaggcccca
     3121 gtcgtcgcgc accgagcggg cggcgtcctc ggcgtcccgc aggtccccga cgtggtgcgg
     3181 cgcgctgcgc ggctgctccc agtcgacgct gcgcagatgc agcaggtctt cgatcgtcaa
     3241 gtagcgttcg agcatgtgaa tcgtgcgggc ttcgagcgtt gcttcttcac gcgccgagga
     3301 tccggctttt ttccggaagt ccacgccctc cagggcgatc tcatcctctg aaagaaggta
     3361 gtcttcggtc acgccaagcg agtcggcgag ggccatgagc gcgcgagagc ccggcatatc
     3421 ctcattgcgc tcatacttgc caatggcctg ggcagacacc accccgccca tcacctcggc
     3481 caggccacgg agggacagcc cggccgcggc gcgcgcgacc ttgattttgt gtccaatcat
     3541 cgtccacctc ctgcatcacg tcggtttaca tatggcgtat gtagtgtgtt ttttgtaaac
     3601 tgataccctt gaaatcggct cttggggctc catatgagcc tgagcccgat aataaggcga
     3661 cattttgtaa accgcgcgat cgaccgattg tcggcaacgg aaagggggcg ccaggtggcg
     3721 ttgagcaaca ctgaactgcg ctattacgac ggcaacgtgc tgcgcctgcc ggccgacaag
     3781 cgcaaagaat accgcgccca ggtcgaccgc ctgatcaccg agttgtgcaa gcgcgtccgc
     3841 gacaagaccg agatcannnn nnnnnngatc acgaaggtgg tcaaggccgg atcgttcgcg
     3901 aaatacacca ttctgcgcaa gactgcggtc gacccggtcg atgtcgatgt ggtgttctac
     3961 atctcgggcc gtgacatcaa tcacgagacc ctcaagagca tcaacgacca gatctatgac
     4021 ctgctgatca agatctaccc gaccaagtcg gtcgaggatt tcgagatcca gcgcaaggcg
     4081 gcgacggtga cgtttgtcgg cagcggcctc agcgtcgata tcgtccccgt catcgaggat
     4141 ccgaaccagg ctggctatgg ctggcagttc gacatccatg atggctccaa ggtccagacc
     4201 tgcgcgccgt gtcagatcaa gttcgttcgc gaccgcaagg accaggacaa ggactttcgc
     4261 acgttggtgc
//
LOCUS       DAOMEO010000117         4251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2521_length_4251_cov_2.265253, whole genome shotgun sequence.
ACCESSION   DAOMEO010000117 DAOMEO010000000
VERSION     DAOMEO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2521_length_4251_cov_2.265253"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..512
                     /locus_tag="PLH31_03735"
     CDS             156..512
                     /locus_tag="PLH31_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQR88373.1"
                     /translation="MTRTKLVLGLSVAALISAAAIGGVAVAANARSEKGERDEIAAVN
                     GARVSLAQAVAIAERQGSGKAMEVSFDDESGRNWEVEIVSGDRVATYAVDAASGDVSA
                     VAEEVDEANEGPKDND"
     gene            565..1242
                     /locus_tag="PLH31_03740"
     CDS             565..1242
                     /locus_tag="PLH31_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQR88374.1"
                     /translation="MKLLLLEDDAEAAEFVQRGLTEAGHTVDIAATADDGLHLALEGA
                     HDVLIIDRMVPGGDGLSVVRAVREAGKATPVIFLTAVAGVRDRVEGLESGGDDYLVKP
                     YAFSELLARVQALGRRSPTIDLRTRLEVGDLSLDLGGRTARRGATRLDLTPQEFKLLE
                     YLVRHSGQVVTRAMLLDKIWGFHFDPRTNLVDAHMSRLRSKVDRPFSRELIHTVRGAG
                     YMIDDRA"
     gene            1313..1672
                     /locus_tag="PLH31_03745"
     CDS             1313..1672
                     /locus_tag="PLH31_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88375.1"
                     /translation="MFGAFQWAVTGYANSSLEDDLRLETRLLLTDGGASDLAALQRRI
                     SARTRIASPFAYALFDATGRRLGGQLPVGISCDPGLNQVSVPAPSDDGAIDDEPILVR
                     SLATSLPEGVLVVGKST"
     gene            1697..2617
                     /locus_tag="PLH31_03750"
     CDS             1697..2617
                     /locus_tag="PLH31_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR88376.1"
                     /translation="MAEIALWGAFGMAGLAAVGGLTAGLILARRLDKVNAATARVMQG
                     HLGDRLPPIGLGSEFATLRDNLNGMLERLEASLQAMRHVSSDIAHDLRTPLNRMRQRL
                     EQARGHTRTVKGLQAAIDQALTDLDGALDIFAALLRIAQIEAGTGRETFAPVSASGLV
                     RRLFEAYQPVAELAGHTMALLTDSDREILGDEDLLVQMLSNLVENAIVHADGPLHIIL
                     GVSDEGTRTVLLVTDTGSGIPEEALPKVTRRFFRIDHSRHAPGSGLGLALVAAIATLH
                     DADLVLRSSRPGLSARIAFGPAVVDRTSPP"
     assembly_gap    2677..2686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2759..3466
                     /locus_tag="PLH31_03755"
     CDS             2759..3466
                     /locus_tag="PLH31_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88377.1"
                     /translation="MIRITQSLAGAAAIAVLAGPLAACNPQAGTASPPAMANAASPAV
                     SDRNVRVLAAAEDYETLTETAFDKTFPELSVSLTAARQKASKARAVLAPDQATRLDAL
                     GVEIDAALRDQNRSAIAIAAVETFRVLVSAQDASAQIPVEVSLLDYAGFRYDANAKSR
                     PINWDQMTADLAYARETWARISPRVTAKGLSGAFDSALSGMEAAAAQKNAVAAGIAVS
                     NELALVDALEEHFKPAN"
     gene            3611..>4251
                     /locus_tag="PLH31_03760"
     CDS             3611..>4251
                     /locus_tag="PLH31_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88378.1"
                     /translation="MVLALVVLGGCVSYRSAGLPDSPRPDPSTTDLARAAARLDHPRL
                     APVDIDLAGPLSPQQLGVIAVIANPDLRAQRARLGVAEAQVFEAGLLPDPQFSFSLDR
                     PVSASGLVDAIASGLGFDTSSLYTRPVRQRTARASRNQVRLDVAWLEWQAAGQARLLA
                     ARIGGLETVRGLAFEASRAADDALDRTLRAVARGDLSANDLEARRISAADAGDR"
BASE COUNT          696 a         1405 c         1376 g          764 t
ORIGIN      
        1 ggcttcatgc ctccctgagt cgaaacaacg caatcgtcct cgcctggaca tccgctctcg
       61 tatcctgcgg tatttgtaat gtcgttgtga ccattggcga aggttgactg gtctagtgtt
      121 cgactacgcc cgatgctggg cgaaggagat gtcacatgac ccgcacgaaa ctcgttctcg
      181 gtttgtcggt ggcggcgctg atctcagctg cggctatcgg cggggtggcc gttgccgcca
      241 acgcccgatc cgagaagggc gaacgcgatg agatcgccgc cgtcaacggc gcgcgcgtct
      301 ctctggcgca ggccgtcgca atcgccgagc gtcagggatc gggcaaggcc atggaggtct
      361 cgttcgacga cgaaagcggc cgcaactggg aagtcgagat cgtcagcggt gaccgggtcg
      421 cgacctatgc tgtggacgcc gctagcggcg acgtcagtgc ggtcgccgaa gaagtcgacg
      481 aggccaacga aggtcccaaa gacaacgatt gaccaagacg ccgtcccctc accggagacg
      541 gcgctccctc gcctaggcgc tcagatgaaa cttctcctgc tcgaagatga cgcggaggcc
      601 gccgagttcg tgcaacgcgg gcttacggag gcaggccata cagtcgatat cgccgcgacc
      661 gccgacgacg ggctgcatct ggcgctcgag ggtgcccatg acgtgctgat tatcgatcgc
      721 atggtcccgg gcggcgacgg cctgtccgtc gtccgcgccg tccgcgaggc cggcaaggcc
      781 actcccgtga tcttcctgac ggcggtcgcc ggcgtccggg accgggtcga aggcctcgaa
      841 tccggcgggg acgactatct ggtcaaaccc tatgccttca gcgaactcct ggcgcgggtt
      901 caggccctcg ggcggcgatc gcccaccatc gacctgagaa ccagactcga ggtcggcgat
      961 ctgtcgctcg acctcggcgg gcggaccgcc cggcgtggag cgacgcggct ggatctgacg
     1021 cctcaggagt tcaaacttct ggaatacctg gtccggcata gcggccaggt ggtcacccgc
     1081 gccatgctgc tggataaaat ctggggattc catttcgatc cccggaccaa tcttgtcgac
     1141 gcgcacatga gcaggctgag gtccaaggtc gaccgcccat tcagccggga gctgatccat
     1201 acggttcgcg gggccggata catgatcgat gaccgggcct agatttctct cctcctcggc
     1261 gctgcggcta gcggccgctt tcgccctgat cttcgccttg ggcggagcct tgatgttcgg
     1321 agcgttccaa tgggccgtca ccggttacgc caacagctcg cttgaggacg atcttcgtct
     1381 tgagacaaga cttttactga ccgacggcgg ggcgtctgat ctcgccgcac tgcagcgacg
     1441 gatctccgct cggacgcgta tcgccagccc gtttgcctat gcgctgttcg atgccaccgg
     1501 ccgccggcta ggcggccagt tgccggtggg aatttcttgc gaccctggct tgaaccaggt
     1561 ttccgtgcct gcgccgtctg acgatggagc aatcgacgac gaacctatcc tggtgcgcag
     1621 cctcgcgacc tccttgcccg agggcgtgct cgtggttggc aagtcgacct aggcggtcta
     1681 cgagctgcag gaatggatgg cggagatcgc tctatggggg gccttcggca tggcggggct
     1741 cgccgctgtc ggcggtttga cggcgggtct gatcctggcc cggagactcg acaaggtgaa
     1801 cgctgcgacc gcaagggtca tgcaagggca tctgggcgat cgtctgccgc cgatcggtct
     1861 gggctccgaa ttcgcgacgc tgcgggacaa ccttaatggc atgctggaac gcctcgaagc
     1921 ctccctgcag gcgatgcggc acgtctcgtc cgacatcgct cacgacctcc gcacgccgtt
     1981 gaaccgcatg cgccagcgcc tggagcaggc gcgcggacat acaagaacgg tcaagggcct
     2041 gcaggccgcc atcgatcagg ccctgacgga cctggatggc gcattggaca ttttcgctgc
     2101 cctgcttcgc atcgcccaga tcgaggcggg aacgggacgg gaaaccttcg ctccggtgtc
     2161 cgcttcgggg cttgtccggc ggttgttcga ggcctatcag ccggtcgccg agctagccgg
     2221 tcacaccatg gctctgctga ccgacagcga cagggaaatc ctcggcgacg aggatctgct
     2281 ggttcagatg ctatccaatc tggtggagaa cgccatcgtc cacgcggacg gtccgctcca
     2341 catcatattg ggcgtctccg acgaaggaac gagaaccgtc cttttagtca ccgatacagg
     2401 atcaggaatt ccggaagagg cgttgccgaa agtgaccagg cgcttcttcc gaatagacca
     2461 tagccgtcac gcaccgggct cgggtttggg actcgccctg gtcgcggcga tcgctacgct
     2521 gcacgacgcc gacctggtgc ttaggtccag ccgtcctgga ctgagcgccc ggatcgcctt
     2581 tggcccagct gtcgtggaca ggacctcccc gccatgacac gctccctgtt gagcgccgat
     2641 gggtttccag ggcaggcgca ctggctcccg tcaatcnnnn nnnnnnaggg ccgcccgccg
     2701 cttcgtgtga tacccccggc ccgtcctcgc cgccgtcctt tccaaggagc ccagagtcat
     2761 gatccgcatc acccaatccc tcgctggcgc cgccgcgatc gcggtcctcg ccggccccct
     2821 cgccgcatgc aatccgcaag ccggcacggc gagccctccg gctatggcga acgcagcctc
     2881 gcctgcagtg tcggatcgca acgtccgcgt tctggccgcg gccgaagatt acgagacgct
     2941 gacggaaacc gctttcgaca agacctttcc tgagctttcc gtatctctga ccgctgcccg
     3001 acagaaggct tccaaggccc gggccgtgct cgcgccggac caggccacgc gcctggacgc
     3061 gctgggcgtc gagatcgacg ccgccttgcg ggatcagaac cgttcggcga tcgcgatcgc
     3121 ggcggttgaa acattcaggg tcctagtgag cgctcaggac gcctcggccc agatccccgt
     3181 cgaagtcagc ctgctagact atgcgggttt cagatacgac gccaacgcca agtcgcggcc
     3241 tatcaattgg gatcagatga ccgccgacct ggcctacgcc cgcgaaacct gggcgcgcat
     3301 ctctccgcgc gtcaccgcca agggcctgag cggcgcgttc gacagcgccc tctcgggaat
     3361 ggaggccgca gccgcgcaga agaatgcggt cgccgcgggg atcgccgtat cgaacgagtt
     3421 ggcgctggtc gatgccctgg aagagcattt caagccggcg aactagcgct atcttcgcgt
     3481 ctcgttggtc gggacgggca aaatcggtcg ctggaggcct agtgtgaatg cgttgacccc
     3541 ttcgcccgac aaagggacgg acggccctgt ctcggacgtc aggcaacgtc ggacgccggg
     3601 tttggcggcc gtggttctgg cgctcgtcgt tcttggcggt tgcgtctcct atcgatccgc
     3661 cggactgccg gacagcccgc gtcccgatcc ctcgacgacc gacctggctc gggcggccgc
     3721 ccggctggac catccccggc tcgcccccgt ggacattgat ctggcgggac cgctttcgcc
     3781 gcagcagctt ggtgtcattg ccgttatcgc caatcccgac ctgagggccc aacgtgcccg
     3841 gctcggcgtt gccgaggcgc aggtgttcga ggccggcctg ctgccggacc cccagttttc
     3901 cttcagtctg gaccgtcctg tttcggccag cggcctcgta gacgccatcg ccagcggcct
     3961 cggtttcgat acctccagcc tctacacacg cccggttcgg cagaggacgg ctagggcgag
     4021 ccggaatcag gttcggctag acgtcgcctg gctggaatgg caggcggcgg gccaggcgcg
     4081 cctactggcc gcccgcatcg gagggctgga aacggtccgg gggctggctt tcgaagcctc
     4141 tcgcgccgcc gatgacgcct tggatcggac acttcgggcg gtggcgcgcg gcgacctgtc
     4201 ggcgaacgac ctggaagctc gacgcatatc ggcggccgac gccggcgacc g
//
LOCUS       DAOMEO010000118         4245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2527_length_4245_cov_2.725776, whole genome shotgun sequence.
ACCESSION   DAOMEO010000118 DAOMEO010000000
VERSION     DAOMEO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_4245_cov_2.725776"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLH31_03765"
     CDS             complement(<1..768)
                     /locus_tag="PLH31_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR88379.1"
                     /translation="MSTARTAFYGPADHDLAPVAADAIQVSPLVIGATDLASIADQSL
                     DAIAIRAPAGVVERRFVLAHALRALTVEGRLTAFGPKDRGGLRLKKELEAFGCVVGES
                     SKKHNRICVALRPAEPVGLAEAIEAGAPRRASDDLWTQPGVFSWDRLDPGTALLLKVL
                     PELSGAGADFGCGIGWLARSVLTSPKVTKLTLVDLDRRAIDAACRNIEDPRAEFVWDD
                     VRQTASTLEKLDFVVTNPPFHETGGEDRSLGQAFIKTA"
     gene            complement(828..917)
                     /locus_tag="PLH31_03770"
     tRNA            complement(828..917)
                     /locus_tag="PLH31_03770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Ser,seq:tga)
     gene            1023..1934
                     /locus_tag="PLH31_03775"
     CDS             1023..1934
                     /locus_tag="PLH31_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQR88380.1"
                     /translation="MVVDKHLWRGLRVASLVALAAASLAACSTPKPRLETRIDSKRHI
                     ASSPRGRMVSPSGKPLRGTEKPYQIRGIWYYPQADPDYDVKGVGSWYGEQFHNRRTAN
                     GEIFDMNALSAAHKTLPLPSLVEVTNLDNGRKMVVRVNDRGPFVGDRIIDLSRAAADK
                     LGYRRQGVARVRVRYIGPAPKSAFDSRQYAQAPQQPPPPRQDPPRDAAPLTWPDEPPP
                     VRVQVIPSRQAGFRIQAGAFSSRENAERAVAQLQSAGRAVIEPMARASGTLYRVTVSA
                     GEDELSAWSLRDRVVALGYSGATVLRP"
     gene            1989..2615
                     /gene="tmk"
                     /locus_tag="PLH31_03780"
     CDS             1989..2615
                     /gene="tmk"
                     /locus_tag="PLH31_03780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQR88381.1"
                     /translation="MTRGAFISFEGGEGAGKSTQIRRLADRLKATGFDVVLTREPGGS
                     PGAETIRDLLVNGDADRWSSVTETLLMYAARRDHLERVIRPALDRGAMVLCDRFADST
                     RAYQGAGGDAPASLIAALEDHVLAGSIPDLTLIFDIPAMIGLDRAASRGGAARFESKG
                     LEFHERLRAGFLAIAQREPERCAVIDANATPDAVFETVWAVVSTRLDL"
     gene            2617..3621
                     /locus_tag="PLH31_03785"
     CDS             2617..3621
                     /locus_tag="PLH31_03785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQR88382.1"
                     /translation="MDAPPHPRDVYGLEGLGAAEAAFLDALARGRLHHAWLLVGPEGV
                     GKATLAYRMARRLLGARPDPSQGLLGAAPSDVVSRQVAARSHPDLMVLERATDDGKAR
                     KSIPVDEARRLPEFFANSPAVSPYRVAIIDAADDLNVNAANAVLKTLEEPPARGVILL
                     VSHAPGKLLPTIRSRCRRLVVPAPGVEAATSMVEQRAGLDHRDAERLARMAHGAPGKA
                     LQLASSGALAMDDAASEILRGLPAVDEGALLALADTFRGGEGAARFELLFDRLADQVH
                     AMTTRLAATGSSPALDRWAAVWERLSRWPGEAEAINLDRADVLWSAISDLRAAAKTAM
                     "
     gene            3618..>4245
                     /locus_tag="PLH31_03790"
     CDS             3618..>4245
                     /locus_tag="PLH31_03790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQR88383.1"
                     /translation="MSRMLIDSHVNLHAHQYDEDRDAVIARARDAGIGLMVTICDKVS
                     SFEAVHAIALAEPDIWCTVGTHPHEAKENPELTANALVELAGRPRVIGIGECGLDFHY
                     DFSPREVQAEVFRVHATAARQTGLPLVVHTREADDVMAQILEEEHARGAFKLLMHCYT
                     SGPELAARAAALGAWFSVSGIATFKAAEAVRAIIRDMPADRIIVETDCP"
BASE COUNT          714 a         1419 c         1374 g          738 t
ORIGIN      
        1 ggccgtcttg ataaaggcct gccccagcga gcgatcttca ccgccggttt catgaaacgg
       61 cgggttcgtt acgacaaaat cgagcttctc cagtgtcgaa gcggtttgcc gcacgtcgtc
      121 ccacacgaac tcggcgcgtg ggtcttcaat attgcggcag gctgcgtcaa tcgcgcggcg
      181 atccagatcg accagcgtca acttagtgac cttgggcgag gtcagcacgc tgcgcgccag
      241 ccagccaata ccgcagccaa agtccgcgcc tgccccggag agctccggca gcactttcaa
      301 gagcaaggcc gtgcccggat caaggcgatc ccaactgaac acgccgggct gcgtccaaag
      361 atcatcggag gcccgacgcg gcgcgccggc ttcgatcgct tcggccagcc cgacgggttc
      421 cgccggacga agcgcgacgc agatacggtt gtgcttcttg gagctttcac ccacgacgca
      481 accgaaagcc tcgagctcct tcttcagtcg cagtccgccg cgatccttgg gaccaaaggc
      541 ggtcaggcga ccctcgaccg tcagagcgcg cagcgcatgg gccagaacga accgtcgttc
      601 cacgacaccc gccggcgccc ggatcgcgat tgcgtccagc gactggtcag caatagacgc
      661 cagatcggtg gccccgatca ccaggggaga gacctggatt gcgtccgcag cgaccggcgc
      721 gaggtcgtga tcggccgggc cgtagaacgc ggtgcgagct gtagacatcg gtatcctgaa
      781 ggttccgccg cgctggaaat ctgttcgacc agcgcggcgg ggaagggtgg cggacagagt
      841 gggattcgaa cccacggtag actttcacct acggcggttt tcaagaccgc tgccttaaac
      901 cactcggcca tctgtccgga gcgagcggct atatcagcgc gcccgcgcct taaccagact
      961 tcaaaacaac tgatgcatca tcgaggcttg gaagggcgcg cccgcgtcgg aaagacgtca
     1021 gcatggttgt ggacaagcat ctttggcgcg gcctccgcgt ggccagcctg gtggccctgg
     1081 ccgcagcgag cctcgccgca tgctcgacgc caaagccgcg tctcgagacg cgtatagata
     1141 gcaagcgcca cattgcctct tcgccgcgcg gccggatggt ctcgccgtcc ggcaagcctt
     1201 tgcggggaac tgagaagcca tatcagatca gaggtatctg gtactatcct caggcggacc
     1261 ctgattatga cgtcaagggc gtcggctctt ggtatggcga gcaattccac aaccgccgaa
     1321 ccgccaatgg cgagatcttc gacatgaacg ccctgtcagc ggcccacaag accttgccat
     1381 tgcccagtct ggttgaggtg accaatctcg ataacggtcg caagatggtc gtccgcgtca
     1441 atgaccgggg accgttcgtc ggcgatcgga tcatcgatct gtcccgcgcg gcggccgaca
     1501 agttgggcta tcgccgccag ggcgtggccc gcgtgcgggt ccgctacatt ggtcctgcgc
     1561 ccaagagcgc attcgactcc cggcaatatg cgcaggcgcc ccaacagccg ccgccgccca
     1621 ggcaagaccc tccgcgggac gccgcacccc tgacctggcc ggacgagccg ccgccggttc
     1681 gcgtgcaggt cattccgagc cgtcaggccg gtttcagaat ccaggcgggg gcattttcca
     1741 gtcgggagaa tgccgagcgt gcggtggcgc aactgcaaag cgccgggcgt gccgtcatcg
     1801 agcccatggc gcgcgccagt ggtacgcttt accgggtcac ggtttccgcg ggagaggacg
     1861 agctttccgc ctggtcgctg cgcgatcgcg ttgtcgcgct gggctattcg ggcgcgacgg
     1921 ttctgcggcc ctgatcgaac gcggcgagac gactggacaa gtgatgcgat ccggttgaat
     1981 tgagaccggt gacgcgcggt gcattcatca gtttcgaggg cggtgagggg gctggaaagt
     2041 cgacccagat ccgtcgcttg gccgatcgcc tcaaagcgac cggctttgac gtggtgctga
     2101 cccgcgaacc gggcggcagc cctggcgccg aaacgatccg cgacctgctg gtcaatggcg
     2161 acgccgaccg ctggtcgtcc gtaacggaaa ccctcctgat gtacgccgcg cggcgcgacc
     2221 atctggagcg cgttattcgg cccgccctgg accgcggcgc gatggtgctg tgcgaccggt
     2281 tcgccgactc gacgcgcgcc tatcaaggag ctggcggaga cgcgcccgcg tctctgatcg
     2341 cggcgctgga ggatcatgtc ctggcaggtt cgatcccgga cctgaccctg atcttcgaca
     2401 tacccgccat gattggtctg gatcgggcgg cctcgcgtgg cggcgccgcg cgtttcgagt
     2461 ccaagggcct ggaattccat gaacggctgc gcgccggttt cctggccatc gcccagcgag
     2521 aacccgaacg ctgcgctgtc atcgacgcca acgccacacc tgatgcggtc ttcgagaccg
     2581 tctgggcggt cgtctcgacc cgtctcgacc tctgatatgg acgcgcctcc ccatcctcgt
     2641 gatgtttacg gactggaggg tctcggcgcc gccgaagccg cttttctcga cgctctggcg
     2701 cgaggccgtc tgcaccacgc ctggctgctc gtcggtcccg agggtgtggg caaggcgacc
     2761 ttggcctatc gcatggcccg ccgcttgctc ggcgcgcggc cggatccctc tcaggggctg
     2821 ctcggcgccg cgccgtctga cgtggtcagt cgacaggtcg ccgcccgttc acatcctgac
     2881 ctgatggtgt tggagcgcgc caccgacgat ggcaaggcgc gcaagtcgat cccggtcgac
     2941 gaggcgcggc gcctgcccga gttcttcgcc aattcgccgg cggtctcgcc ctatcgcgtc
     3001 gccattatcg acgccgccga cgacctcaac gtcaacgccg ccaacgcggt gctgaagacg
     3061 cttgaggagc cgccagcccg gggcgtcatc ctgctggtca gccacgcgcc aggaaagctg
     3121 ttgccgacca tccgttcgcg atgccgccgg ctcgtcgtcc cggcgcccgg cgtcgaagcc
     3181 gccaccagca tggtggagca acgggcaggt ctggaccatc gggacgccga acgtctggcc
     3241 cgcatggcgc acggcgcgcc aggcaaggcg ctgcaactgg cctcctccgg cgccctggcc
     3301 atggacgatg cggcgagcga gatcctgcgc ggtctgccag ccgtggacga aggcgctttg
     3361 ctggccctgg ccgacacgtt caggggcggt gagggcgcgg ctcgttttga gctattattc
     3421 gatcgcttag ccgatcaggt tcatgccatg accacccgct tggcggcgac gggctcttct
     3481 cccgcgctgg atcgttgggc cgcggtctgg gagcgcctgt cacgatggcc aggagaagcc
     3541 gaagcgatca atctcgatcg cgcggatgtc ctctggagcg ccatctcgga cttgcgcgcg
     3601 gcggccaaga cggcgatgtg agccgcatgc tcatcgacag tcacgtcaat ctccacgccc
     3661 atcagtacga cgaagaccgc gacgcggtga ttgcccgcgc ccgcgacgct ggcatcggct
     3721 tgatggtcac catttgcgac aaggtctcgt cctttgaagc cgtccacgcc atcgccttgg
     3781 ctgagcccga catctggtgc acggtcggca cgcatccgca tgaagccaag gaaaatccgg
     3841 agctgaccgc taacgcgctc gtagagctcg ccggccgccc ccgcgtgatc ggcatcggcg
     3901 aatgcggtct cgatttccac tacgacttct cgcctcgcga ggttcaggcc gaggtgttcc
     3961 gcgtccatgc caccgccgcg cgccaaacgg gcttgccgtt ggtggtccac acccgcgaag
     4021 ccgatgacgt gatggcccag atcctggaag aggagcatgc gcgtggtgcg ttcaagcttc
     4081 tgatgcattg ctacactagc ggcccggagc tcgcagcccg tgcggcggcc ctcggcgcat
     4141 ggttttccgt gtcgggcatc gccaccttca aagcggctga agcggtcagg gcgatcattc
     4201 gcgacatgcc ggccgaccgg attatcgtcg agactgattg cccgt
//
LOCUS       DAOMEO010000119         4241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2530_length_4241_cov_2.588629, whole genome shotgun sequence.
ACCESSION   DAOMEO010000119 DAOMEO010000000
VERSION     DAOMEO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_4241_cov_2.588629"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1210)
                     /locus_tag="PLH31_03795"
     CDS             complement(98..1210)
                     /locus_tag="PLH31_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HQR88384.1"
                     /translation="MSRSFFRTALAVAVTACALVSGPAFAKSRIKDIVSFEGVRDNQL
                     IGYGIVVGLNGSGDSLRNAPMTKQSLEAMLERQGVNVRDANLNTKNVASVMVTANLPA
                     FSAPGSRVDVSVSTLGDAKSLLGGTLLVTSLQGADGQTYAVAQGSVQTGSISAGGASG
                     SSVTKGVPTAGRIAAGGIIEKETGFQMVNMGELRMTLRNPDFTTSRRIADAINGRFAN
                     SAQAQNPTIVAVRPPAGMDMISFITAIENLQVEPDGPAKVIIDEVAGVIVMGDDVRIS
                     QVAIAQGNLTISVQENPAVSQPAPFSQGQTTVVPQTAVNVEEEKGKKLITLNAGASLK
                     SLVGGLNALGVTPRDMISILQAVKAAGALQADIEVM"
     gene            1430..1858
                     /locus_tag="PLH31_03800"
     CDS             1430..1858
                     /locus_tag="PLH31_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HQR88385.1"
                     /translation="MKVSSTGGVGASGASRAKASSSGSSFSLPSVNGASGAASAASVG
                     GLAGVGSLGALLALQANGDPLERRRRQVKRADDILDILGEVKIALLDGDVSTGTLDRL
                     TRAIRQQRESTDDPRLEGVLNEIETRAAVEKAKLEQARGA"
     gene            2094..2522
                     /gene="dksA"
                     /locus_tag="PLH31_03805"
     CDS             2094..2522
                     /gene="dksA"
                     /locus_tag="PLH31_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQR88386.1"
                     /translation="MQTATVLVDKKTYRPHEGEEFMNERQLEYFKGKLNEWKEEILRE
                     SRETVAHLQKETENHADLADRASSETDRALELRTRDRQRKLISKIDQALRRVEDGSYG
                     YCEETGEPIGLARLEARPTATLSLEAQERHERRERVHRDD"
     gene            complement(2593..2946)
                     /locus_tag="PLH31_03810"
     CDS             complement(2593..2946)
                     /locus_tag="PLH31_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HQR88387.1"
                     /translation="MAQLTVWFDGACPLCLREIALMRRLDRAGAIDFLDATGGDVECP
                     IDRADLLARFHAREDGVLLSGAGAFAAMWRAIPLLRPLGLAARQRWVLAVLEFAYRRF
                     LRIRPRLQRLAARWD"
     gene            complement(3054..3665)
                     /locus_tag="PLH31_03815"
     CDS             complement(3054..3665)
                     /locus_tag="PLH31_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQR88388.1"
                     /translation="MMIDSLESLEALYSAAPAAASLVKVSDRITPHYQALIEASPFVA
                     LATVGPEGLDCSPRGDQPGFVRVADPRTLILPDRRGNNRIDSLRNVIRDPRVALLFLI
                     PGSGVTFRVNGRARISADPDLLQSFAIDGKAPRTAMVITVEEAYFQCARAILRAGLWT
                     PEAQVDPASLPSAGAMLAAVTAGEVGGEAYDAAWPERARQTMW"
     gene            complement(3727..4089)
                     /locus_tag="PLH31_03820"
     CDS             complement(3727..4089)
                     /locus_tag="PLH31_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88389.1"
                     /translation="MTVHIKARHALIIEDEILIAFEVEALLTEQGFTSFDIADSPQDA
                     LAMAIARPPDLITADYRIVGGTGVEAVEAIQKQLGAIPVVYVTGNSDLLLGREPPVVG
                     KPISPRRLAEACARAFSN"
BASE COUNT          736 a         1350 c         1467 g          688 t
ORIGIN      
        1 atctggccgc gcttgagcag ctcggccgca gaagcgggcg cggtgacgga ggcctgaagg
       61 ccggtgatcg gcggggcggt gagggtaaga gcgctcatca catcacctcg atgtcggctt
      121 gcagggcgcc ggccgccttc acggcctgca ggatcgagat catgtcgcgc ggggtgacgc
      181 ccagggcgtt gaggccgccg accaggcttt tcagcgacgc gccggcgttg agcgtgatca
      241 gcttcttgcc cttttcctct tcgacattga cggccgtttg cggaacgacc gtggtctggc
      301 cctggctgaa gggggcgggc tggctgacgg ccgggttctc ctggaccgag atggtcaggt
      361 tgccctgggc gattgccacc tggctgatgc ggacgtcgtc gcccatgacg atgacgccgg
      421 ccacttcgtc gatgatcacc ttggccgggc catcgggctc gacctgcagg ttctcgatgg
      481 cggtgatgaa gctgatcatg tccatgccgg ccggcggacg cacggcgacg atggtggggt
      541 tctgggcctg ggcgctgttg gcgaagcggc cgttgatggc gtcggcgatg cggcgcgagg
      601 tggtgaagtc ggggttgcgc agggtcatgc gcagctcgcc catgttcacc atctgaaagc
      661 cggtttcctt ttcgatgatg ccgccggcgg cgatccggcc cgcggtcgga acgcccttgg
      721 tcaccgagct gcccgaggcg ccgccggccg agatcgaacc ggtctgcacg ctgccttgcg
      781 ccaccgcata ggtctggccg tctgcgccct gcaggctggt gaccagcagg gtgccgccca
      841 gcaggctctt ggcgtcgccc agggtggaga ccgaaacatc gacccgcgag cccggcgccg
      901 agaaggccgg caggttggcg gtgaccatga ccgaagccac gttcttggtg ttcagattgg
      961 cgtcacgcac attgacgccc tggcgctcaa gcatggcctc gaggctctgc ttggtcatcg
     1021 gcgcattgcg caggctgtcg cccgagccgt tgaggccgac gacgatgccg tagccgatca
     1081 gctggttgtc gcgcacgcct tcgaacgaga cgatgtcctt gatccgcgac ttggcgaagg
     1141 ccggccccga aaccagggcg caggccgtca cagcgacggc gagagcggtg cggaagaatg
     1201 aacgcgacat ggctatcctg ctgcgggaag acattcccgg ccggctccat gccaaaagcg
     1261 ggccaatctc aaaggcttga gtcgtaaggc tttgtgcgtt ttgacgcagg cccgtgggcg
     1321 cccttcggca gaatctgccc ggcattaacc atacttcaag cggccggacc gattatggtt
     1381 cgacggcgag acgcgtgacg cgcctcaggc actatggagc ggcgttcgca tgaaggtttc
     1441 gagcacgggg ggcgtaggcg cgagcggcgc atcgcgagcc aaggcctcga gcagcggttc
     1501 cagcttttcc ctgccttcgg tcaacggcgc cagcggcgcg gccagcgcgg cttcggtcgg
     1561 cggtctggct ggcgtaggct cgctgggcgc gctcttggcc ctgcaggcca acggcgatcc
     1621 cctggagcgc aggcgccgcc aggtcaagcg agccgatgac attctcgata tcctgggcga
     1681 ggtgaagatc gccctgctcg acggcgacgt ctcgacgggg acgctggatc gactcacccg
     1741 agcgatccgc cagcagcgcg agtcgacgga cgatccgagg ctggaaggcg tgctcaacga
     1801 gattgaaacg cgggcggcgg tggaaaaagc caagctcgaa caggcgcggg gcgcctgatg
     1861 gccgcgacgc cgatcacgga cgatgtctgt catcgcgctg tcagcgaatt caagacgttg
     1921 cgcgctgcgg tttcgcgcgt ttctagcggt gtcgcaggcc ggttttgcag ccgacgacgc
     1981 gagtcgcgtg cgcctgaacg cgccactcac gcgaggttca ttgaacgcgg ctattctttt
     2041 ggtataagca cccagcccga cgagggctga gttcatcggg gcgtgaggcg ttaatgcaaa
     2101 cggccactgt tctagtcgat aaaaagacct atcgtcctca cgagggcgaa gagttcatga
     2161 acgagcgtca gcttgagtat ttcaagggca agctgaacga atggaaagaa gagatccttc
     2221 gcgaatctcg cgaaacggtc gctcatctcc agaaagaaac cgagaaccac gccgatctgg
     2281 ccgatcgcgc gtcctcggaa accgatcggg cgcttgaact gcgcacccgc gatcgccaac
     2341 gcaagctgat ctccaagatc gaccaggccc tgcgccgcgt cgaggacggc tcctatggct
     2401 attgcgagga aaccggcgaa cctatcggcc tggcccgact ggaagcccgt ccgaccgcga
     2461 ccctcagcct ggaggcccag gaacgtcacg aacggcgcga acgcgtccat cgcgacgact
     2521 gaccggcctt aaggcgcctg tgttcaagga cggctccgcg cccaggcgcg gggccgttat
     2581 tgtttttggc cctcaatccc agcgcgcggc caggcgttgc agccgggggc gaatgcgcag
     2641 gaagcggcgg taggcaaatt ccaggaccgc caggacccag cgctgccgag cggccagccc
     2701 aagcggccgc agcaacggaa tggcccgcca catggccgcg aaagcgcccg cgcccgacaa
     2761 aagcaccccg tcctcgcggg cgtggaaccg ggccagcaga tccgcccgat cgatcgggca
     2821 ttcgacatcg ccgcccgttg catcgagaaa atcaatggcg cccgcccggt cgagccgtcg
     2881 catcagggcg atctcgcgga ggcacagcgg gcatgcgcca tcgaaccaga cagtcagttg
     2941 ggccatgact actatctagg gagccgggcg cccgcccgca tcgggcgatt gaagaattag
     3001 ccgctgacgg acgcgtcatc cggcggctcg aagtcagggg agggcgcgcg gcctcaccac
     3061 atcgtctgcc gggcccgttc gggccacgcg gcgtcatagg cctcgccgcc gacttccccg
     3121 gcggtcaccg ccgccagcat cgcgccagcc gagggcaggc tggccggatc aacctgggct
     3181 tcgggcgtcc agagccccgc gcgcaggatc gcccgggcgc actggaaata ggcctcctcg
     3241 acggtgatca ccatcgccgt gcgcggcgcc ttgccgtcga tggcgaagct ctgcagcagg
     3301 tcagggtcgg cgctgatcct cgcgcggcca ttgacccgaa aggtcacgcc cgaacccggg
     3361 atcaggaaca gcagggctac gcgcggatcg cggatcacat tgcgcagcga atcgatgcgg
     3421 ttgttgccgc gccggtcggg caggatcagg gtcctggggt cggcgacgcg cacgaagccg
     3481 ggctggtcgc cgcgcggact gcagtcgaga ccttcaggac cgacggtcgc cagggccacg
     3541 aagggcgagg cctcgatcag cgcctgataa tgcggggtga tcctgtcgga caccttgacc
     3601 agtgaggcgg cggcgggcgc ggcgctgtag agggcttcca gcgactcaag gctgtcgatc
     3661 atcatgggct ggtcctcccg ggtcgtacgg aaagcctagt cggccccggg gcggtcggca
     3721 aagctatcag ttcgaaaagg cccgggcgca ggcctcggcc aggcgccggg gcgagatcgg
     3781 cttgcccacc acgggcggct cgcgtcccag cagcagatcc gaattgccgg tcacatagac
     3841 caccgggatc gcgcccaact gcttctggat cgcctcgacc gcctcgacgc cggtgccgcc
     3901 gacgatccgg tagtcggccg tgatcaggtc gggggggcgg gcgatggcca tggccagggc
     3961 gtcctgcggg ctgtcggcga tatcgaagct ggtaaagccc tgttcagtca gcaaggcttc
     4021 cacctcgaag gcgatcaaaa tctcgtcctc gataatcagg gcatgtcggg ccttgatgtg
     4081 cacggtcatt cgccgctgcc ttctttatcg tcccatcttg atttcaacgc tcgcgcgagg
     4141 tcgtgcgcca gtcgcgtttt cgtgatgtga caaggccgca gtcgccttga gcgcgcacaa
     4201 ggggcgccgg agggtgagaa aacggactcc ggaggcgggc c
//
LOCUS       DAOMEO010000120         4241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2531_length_4241_cov_2.478739, whole genome shotgun sequence.
ACCESSION   DAOMEO010000120 DAOMEO010000000
VERSION     DAOMEO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2531_length_4241_cov_2.478739"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>300
                     /locus_tag="PLH31_03825"
     CDS             <1..>300
                     /locus_tag="PLH31_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88390.1"
                     /translation="TLLTYKTGEGGQGPGGVVRYAGEGSRCRPQTAGVIQYADGTQWI
                     GGVTSIDETPFLPQPEGLGEFKFAEGVSKILRARKTADGSAWEIAEELVDPPPPPP"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..1183)
                     /locus_tag="PLH31_03830"
     CDS             complement(<311..1183)
                     /locus_tag="PLH31_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HQR88391.1"
                     /translation="MPSGLAALLDDVAGITKIAAASLDDMTSAAGKAGTKAVGVVVDD
                     AAVTPGYAMGFTPDRELPIVWKIAIGSFKNKLLFLLPGALLLSAFAPWAVTPILMLGG
                     AYLCFEATEKILEALSGQHSADEPEELALLSGEIMAIALNDVAGKPLVVQAGALALVG
                     ILITIAVYGVVGLIVKMDDIGLHMAQRQNAGVRGFGRGLVKAMPITMEALTIIGTAAM
                     LWVGGGIIVHGLEHFHLTPIPHWVEGFSHWAQGVPGVGAVTGWLAFAVGSAVVGFVVG
                     GALAGVMHLWHHRKG"
     gene            1299..2276
                     /locus_tag="PLH31_03835"
     CDS             1299..2276
                     /locus_tag="PLH31_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQR88392.1"
                     /translation="MNIPAALARLAPHLSARATTAVNARQLSGGASLETWAFDLDDGT
                     PLILRRRPEGAAMRETAAPLAHEAALIVAAARTGAPVPVIEHVCDPEDGLGEAFVMRR
                     LEGETLGKRIVRDAAFDTVRPGLAHRCGQVLAQIHGALLDDLPSLATSDARGELARYE
                     AVYRQHTAERPTFEAAFRWLEASAPEPVTPVLVHGDFRNGNLMVHPEQGLVGVLDWEL
                     AHLGDPAEDLGWICVNSWRFGQWRQPVGGFGDYQALLDGYAAAGGREILLSRLQFWQA
                     LGSLKWGVMCLMMYSSFASGADPSIERAMIGRRVSETEIDLVRLMEAMA"
     gene            2273..2641
                     /locus_tag="PLH31_03840"
     CDS             2273..2641
                     /locus_tag="PLH31_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HQR88393.1"
                     /translation="MITCPTAAELQAALTLFDRDDAGPADPRRVFLARVADNARALLD
                     REATLGEAAEAGERARLAVLLGVEGDFTDLNRQLCDALRSGAIEPRDPALLDHLRATA
                     IARIAIDQPGYCGLEALLGD"
     gene            complement(2608..3762)
                     /locus_tag="PLH31_03845"
     CDS             complement(2608..3762)
                     /locus_tag="PLH31_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HQR88394.1"
                     /translation="MGDRFKGFKRWIFAGAVLFLGLLVAAAFVWRYDILSNALDPKIP
                     FLTYEPPPPPHYAQGASWALRPKDPTSPSAGDPAADVFFVHPTTFDGGQDWNGAIDQP
                     KAQRFLNRVVLPNYAGPFYRVGRVFAPRYRQASLYTYLTLRDDAREARRFAYGDVREA
                     FRLYLARYNQGRPFILVGVEQGGAMSARLLREEIAADPAIKRRLVSAYLIETVVPAEE
                     YGLVSPLPACERREQSGCVAAWASVQEGDFQRIQELTHRSLIWDSGDQLIDLNGRRAL
                     CFNPLLGGISQERAPARLNQGAANATGLEWGARPAFLQRQVWARCQDGLLHTGRPKST
                     SLRDAGSWADRRKVDSFNLFYADIEADAQGRVATLTGRPVQSPSSASSPQ"
     gene            complement(3794..>4241)
                     /locus_tag="PLH31_03850"
     CDS             complement(3794..>4241)
                     /locus_tag="PLH31_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HQR88395.1"
                     /translation="YESQYNGWDGSHFHTARIAAGKAYREAGIERPREQISMMEVHDC
                     FSITELVTMEDLFISPEGQGWRDVLDGFYDADGGVPCQIDGGLKCFGHPIGASGLRML
                     YEMYLQLQGRAGERQLKDPVFGMTHNLGGAPASNVCSVAIIGQEGA"
BASE COUNT          706 a         1431 c         1431 g          663 t
ORIGIN      
        1 gaccctgctg acctacaaga ccggagaagg cggccagggg cccggcgggg tcgtgcgcta
       61 tgccggcgaa ggcagccgct gccgccccca gacggcgggg gtcatccagt atgccgacgg
      121 cacgcagtgg atcggcggcg tcacctcaat cgatgagaca ccttttctcc cgcagcccga
      181 gggtctggga gagttcaagt tcgcggaagg tgtctccaag atcctgcgcg cccgcaagac
      241 ggcggacggc tcggcctggg aaatcgccga agaactggtg gatccgccac cgcctccccc
      301 nnnnnnnnnn gcccttgcgg tgatgccaga gatgcatcac gcccgccagg gcgccgccga
      361 ccacaaagcc gacaaccgct gagcccacgg cgaaggccag ccagcccgta accgcgccaa
      421 caccgggaac gccctgcgcc cagtgggaga agccctccac ccagtggggg atcggcgtca
      481 ggtggaagtg ctcaaggcca tggacgatga tgccgccgcc gacccagagc atggcggccg
      541 ttccgatgat ggtcaaagcc tccatggtga tcggcatggc cttgaccaga ccgcgcccaa
      601 aaccgcgaac accggcattc tggcgctggg ccatgtgcag gccgatatcg tccatcttca
      661 cgatcaggcc gacgacgcca tacacggcga tggtgatcag aataccgacc agcgccaggg
      721 cgccagcctg aacgaccagc ggcttgccgg ccacgtcgtt cagggcgatg gccatgatct
      781 cgcccgacag gagcgccagc tcctcgggct cgtcggcgga gtgctggccg ctcagcgcct
      841 ccaggatctt ctcggtggcc tcgaaacaca gataggcgcc gcccagcatc aggatcggcg
      901 tgacggccca cggcgcgaag gcgctgagca acagggcgcc gggcagcaga aacagcagct
      961 tgttcttgaa cgagccgatc gcgatcttcc agacgatcgg caattcgcgg tccggcgtga
     1021 accccatggc gtagccgggg gtcaccgccg catcatccac cacgaccccg accgccttgg
     1081 tcccggcctt gccggccgcg ctggtcatgt catccagcga ggcggcggcg atcttggtga
     1141 tgccggcgac atcgtccagc agtgcggcga gacctgacgg catgaaactc tcgatcatcg
     1201 gaacagaagg gctgacgcac agggtgtggt gatccgcgtc gaaagtcgac tacgcgatcc
     1261 cttgcgtgac gctgggggag gcctgaaacc taggcgggat gaatattcca gccgccctcg
     1321 cccgccttgc gccgcatctg tcagcgcgcg cgacgaccgc cgtgaacgcc cggcagctgt
     1381 cgggcggagc gagtctcgag acctgggcct tcgatctgga cgacggtacg ccgctgatcc
     1441 tgcggcggcg gcccgaaggc gcggcgatgc gcgagaccgc ggcgcctctg gcccatgagg
     1501 ccgccctgat cgtggcggcc gccagaacgg gcgccccggt tcccgtcatc gagcatgtct
     1561 gcgatccgga ggatggtctg ggcgaggcct ttgtcatgcg ccgcctggaa ggcgagaccc
     1621 tgggcaagcg gatcgtgcgc gatgcggcct tcgacacagt gcggcctggc ttggctcacc
     1681 gctgcggcca ggtactggcc cagatccacg gcgctttgct ggacgacctg ccgtcgctgg
     1741 cgacgtccga cgcccgaggc gaactggcgc ggtacgaggc ggtctatcgc cagcacaccg
     1801 ccgagcgccc caccttcgag gccgccttcc gctggctgga ggcgtccgcg ccagagcccg
     1861 tcacaccggt gctggtccat ggggactttc gcaacggcaa cctgatggtc catcccgagc
     1921 agggtctggt tggcgtgctg gactgggaac tggcccatct gggcgacccg gccgaggatc
     1981 tgggctggat ctgtgtcaat tcctggcgct ttggccagtg gcgccagccc gtgggtggct
     2041 ttggcgatta tcaggccctg ctggacggct atgcagccgc cggcgggcgc gagattctgc
     2101 tgtcgcgcct gcagttctgg caggccctgg gttcgcttaa atggggcgtc atgtgcctga
     2161 tgatgtattc cagcttcgcc agcggcgccg atccgtccat cgagcgggcc atgatcggcc
     2221 ggcgggtcag cgagaccgag atcgaccttg tccgtctgat ggaggccatg gcgtgattac
     2281 ctgtccgacc gccgccgaac tccaggccgc cctgaccctc ttcgatcgag atgacgccgg
     2341 gcctgccgac ccccgacgcg tgttcctggc ccgggtggcc gataatgcgc gggccctgct
     2401 ggaccgcgaa gcgacgttgg gcgaggccgc cgaagccggc gagcgcgcca ggcttgctgt
     2461 cctgctcggc gtcgaaggcg atttcaccga tctgaaccgg caactctgcg acgccctgcg
     2521 gtcaggggcg atagagccgc gcgatccggc cctgctggat cacctgcgcg cgacggcgat
     2581 cgcgcgcatc gccatcgatc agccaggtta ttgcgggctc gaagcgctgc tcggagactg
     2641 aaccggccta cctgtcaggg tcgctacccg gccctgggcg tcggcctcga tatctgcata
     2701 gaacaggttg aagctgtcga ccttgcggcg gtcagcccac gaaccggcgt cgcgcagcga
     2761 ggttgatttg ggccgcccgg tgtgcagcag gccatcctgg catcgcgccc agacctgtcg
     2821 ttgcaggaag gcgggccgtg cgccccattc caggccggtg gcgttggcgg cgccctggtt
     2881 cagacgcgcc ggcgcccgct cctggctgat cccgcccagt agcgggttga agcacagcgc
     2941 ccggcggccg ttcaggtcga tcagctggtc gccggagtcc cagatcagcg agcgatgggt
     3001 cagctcctgg atccgctgga aatcgccttc ctgcaccgag gcccaggcgg cgacgcaccc
     3061 ggactgttcg cggcgctcgc acgctggcag gggcgagacc aggccatact cttcggccgg
     3121 cacgacggtc tcgatcagat aggcggagac caggcggcgc ttgatcgcgg gatcggctgc
     3181 gatctcttcg cgcagcagcc gcgccgacat cgccccgccc tgctcgaccc cgaccaggat
     3241 gaacggccgg ccctggttgt agcgggccag atagagccgg aacgcctcgc gcacatcgcc
     3301 ataggcaaag cgccgcgcct cgcgggcgtc atcgcgcagg gtcagatagg tatagaggct
     3361 agcctggcgg tagcgcgggg cgaacacccg gcccacccga tagaatggcc cggcatagtt
     3421 gggcagcacc acacggttca ggaaccgctg ggccttgggc tgatcgatgg cgccgttcca
     3481 gtcctggccg ccgtcaaagg tggtggggtg gacgaagaac acatcggccg ccgggtcgcc
     3541 tgctgaagga ctggtcggat ctttgggcct cagcgcccag gacgcgcctt gcgcatagtg
     3601 cggcggcggc ggcggctcat aggtcaggaa cggtatcttg ggatcgagcg cgttgctgag
     3661 gatgtcgtag cgccacacaa aggcggcagc caccagcagc cccaggaaga gcaccgctcc
     3721 ggcgaagatc cagcgcttga agcccttgaa gcggtcaccc atgagcttgg gttacaccaa
     3781 gcagggctgg ggattaagcc ccctcctgcc cgatgatcgc caccgagcac acattgctgg
     3841 ccggcgcgcc gcccagattg tgggtcatgc cgaaaaccgg gtcttttagc tgtcgctcgc
     3901 cggcccggcc ctgcagctgc aggtacatct cgtacagcat gcgcagaccc gaggccccga
     3961 tcgggtgacc aaagcatttc aggccgccat cgatctggca cggcaccccg ccgtcggcgt
     4021 catagaagcc gtccagcacg tcgcgccagc cctggccctc gggcgagatg aacaggtcct
     4081 ccatcgtcac cagctcggtg atcgagaagc agtcatggac ctccatcatc gagatctgct
     4141 cgcgcggccg ctcgatgccg gcctcgcgat aggccttgcc ggcggcgatg cgcgcggtgt
     4201 ggaaatggct gccgtcccag ccgttgtact ggctctcata g
//
LOCUS       DAOMEO010000121         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2535_length_4234_cov_2.562814, whole genome shotgun sequence.
ACCESSION   DAOMEO010000121 DAOMEO010000000
VERSION     DAOMEO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_4234_cov_2.562814"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLH31_03855"
     CDS             <1..324
                     /locus_tag="PLH31_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88396.1"
                     /translation="EEATMQAIVDVHYPGIKQKLVAEALRIFYDMRKVPGLKKKPSTS
                     ELLDWLKLLMVEDIDVSLLREKDPTKLIPPLHGALLKNEQDVHLFERLAFLARREGAG
                     GRPGQ"
     gene            415..1191
                     /locus_tag="PLH31_03860"
     CDS             415..1191
                     /locus_tag="PLH31_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88397.1"
                     /translation="MRQSTWIAVLATVGVMALTGCQRTETARTDQSERRAGRDPVRVI
                     SRLDCPENQGDLKRVSVAADGLSCAYAGIDAEVQLRLVSLNGGDAEDALAPIEADLKT
                     LMPSVTPVTELKAGDGRDRNKTSIHLPGVRIDASDGGADIKIGNITINSNEGGASEVK
                     INKNVTARTDEAGDGAVSIRASDDGAEVRHSKRGDSIRSTLILASDKATSSYRVVGYE
                     ARGPKEGPLAIATIKAKNRDSGDHDVFNDMKALVRHNVGG"
     gene            complement(1194..1565)
                     /locus_tag="PLH31_03865"
     CDS             complement(1194..1565)
                     /locus_tag="PLH31_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88398.1"
                     /translation="MSLSRIVMRLARNPGTEFAGGDDHRGYALTAPLTADGHLDEAAY
                     AKSKASCSVRRFAPDEEAVDGLLNRKGTRWFFDYDDSDNSDDEPVHRLGEHRFALGEY
                     VTVTDEDGRPLTYKVMEVVPV"
     gene            complement(1599..2411)
                     /locus_tag="PLH31_03870"
     CDS             complement(1599..2411)
                     /locus_tag="PLH31_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR88399.1"
                     /translation="MTWARIMAPLAGAQADRELLTAAATLAAAFDAELACVHAPADMA
                     DLMPWMGEGFMGGVQVTALERLKEAAIEGARAVGALAAEIGYQRTRVISLDSPVWAGL
                     AMEGRLSDVIVFDNAAARGKGPLAEAFQQLVADEQRPVVVARPGMKVGGVALVAWDGG
                     KEASRAMRTALPLLQKASSVIVAGAPGASSRNFDLGRLVEFLSARGVTATSQTLSGTG
                     DAAGLLLGTALEVGADFLVAGAFGHPRLQEFIFGGTTRSLLNSDGPSLFLSH"
     gene            2518..3162
                     /locus_tag="PLH31_03875"
     CDS             2518..3162
                     /locus_tag="PLH31_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HQR88400.1"
                     /translation="MAKGLHRGAAPRAWQRMLSGRRLDLLDPSPMDIEIEDIAHGLAR
                     VARWNGQTVGEHGFSVAQHSLVVEEIAAHIKPDLEPKWRLAALLHDASEYVIGDMISP
                     FKAALGVSYKDFEARLEDAIHIRFGLPVKTPPAIKKLIKQADRACAFFEATQLAGFDH
                     AESLSIFGAPPVGYELHIKPQPPFEAQAHYVQRFHLLSRAAGFESAPNVAFETE"
     gene            3086..4139
                     /locus_tag="PLH31_03880"
                     /pseudo
     CDS             3086..4139
                     /locus_tag="PLH31_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
BASE COUNT          742 a         1429 c         1375 g          688 t
ORIGIN      
        1 gaggaggcga cgatgcaggc gatcgtcgat gtccactatc cgggcatcaa gcaaaagctc
       61 gtcgccgagg cgctgcggat tttttacgac atgcgcaagg tgcccggcct gaagaagaag
      121 ccctcgacgt ccgaactcct cgactggctc aaactgctga tggtcgagga catcgacgtg
      181 agcctgctac gcgaaaagga tccgaccaag ctgattccgc cgctgcacgg cgccctgctg
      241 aagaatgagc aggacgtgca cctgttcgaa cgcctggcct tcctggcccg gcgcgaaggc
      301 gcaggcgggc gtcccgggca atagcgcctg acagggaatt cactggacgg ccggcgcgac
      361 cacccgcacc ctcccgctga agatagaggg gccgctgaaa acaaggggat ggtcatgcgc
      421 caatcgactt ggatcgccgt acttgcaacc gtcggtgtca tggctctgac gggctgccag
      481 cggaccgaaa cggccaggac cgaccagagc gagcgtcgtg cgggccgcga tcctgtgcgc
      541 gtaatatcca ggttggattg tcctgaaaat cagggcgacc tgaagcgtgt cagcgtggct
      601 gcggacggct tgtcctgcgc ctatgccggc atcgacgccg aggtgcagct gcggctagta
      661 tccctgaacg gtggcgacgc cgaggacgcc ctcgcgccga tcgaggctga cctgaaaacg
      721 ctgatgccct ccgtcacccc cgtcaccgaa ctgaaggctg gcgacgggcg cgatcggaac
      781 aagaccagta ttcaccttcc tggcgtacgc atcgacgcca gtgacggcgg cgcggatatc
      841 aagatcggca acatcacgat caacagcaac gaaggcggcg cgtccgaggt caagatcaac
      901 aagaacgtca ccgcccggac ggacgaggct ggcgacggcg ccgtcagcat tcgcgccagt
      961 gatgacggcg ccgaagtccg gcacagcaag cgcggcgact cgatccgctc gacgctgatc
     1021 ctggccagcg acaaggcgac cagcagctac cgagtcgtgg gttacgaggc gcgcggcccc
     1081 aaggaggggc cgctcgccat cgccaccatc aaggccaaga atcgcgactc cggcgaccat
     1141 gacgtcttca acgacatgaa agcgctagtc cgccacaatg tcggcggctg aaatcagacc
     1201 gggacgacct ccatcacctt gtaggtcagc ggccggccgt cctcgtcggt caccgtgacg
     1261 tattctccca gggcgaacct gtgctcgccc agtcggtgga cgggttcgtc atcgctgttg
     1321 tcagagtcat catagtcgaa gaaccagcgc gttccctttc ggttgagtag gccatcgacg
     1381 gcctcctcgt cgggcgcgaa ccgccggacg ctgcaagagg ccttgctctt ggcataggcc
     1441 gcctcgtcga ggtggccgtc agcggtgagc ggcgcggtca gggcgtagcc gcggtgatcg
     1501 tcgccaccgg cgaactcggt gccagggttg cgggcaaggc gcatcacgat gcgagacagg
     1561 gacatttaag cctccctttg attgaaaatg actgggcttt agtgggagag aaacagcgac
     1621 gggccgtcgc tgttcaggag actgcgtgtc gttccaccga agatgaactc ctgcaggcgc
     1681 gggtgcccga aggccccggc gaccaggaaa tcagctccga cctcaagcgc cgtgcccagc
     1741 aacaggccgg cggcgtcgcc tgtgcccgac aaggtctggc ttgtcgctgt caccccgcgc
     1801 gcggacagga attcaaccag acggcccagg tcaaaattgc gcgaagaggc gcccggggcg
     1861 ccggcgacga tcacggacga ggccttttgc aagagcggaa gcgcggtgcg catcgcccgg
     1921 ctcgcctcct tgccgccatc ccaggcgacc agggccaccc cgccaacctt catgccggga
     1981 cgagccacga cgacggggcg ctgctcgtcg gcgaccagtt gctgaaaggc ctcggccagg
     2041 gggcccttgc cccgcgcggc ggcgttgtcg aagacgatga cgtccgaaag gcgcccttcc
     2101 atcgccagcc ccgcccagac cggcgagtcg aggctgatga cgcgggttcg ctggtagccg
     2161 atctcggcgg cgagcgcgcc gacagcgcgt gcgccttcga tggccgcctc cttcagtctt
     2221 tccagcgcag tcacctgcac accgcccatg aagccctcac ccatccaggg catcaggtcg
     2281 gccatgtcgg cgggcgcatg gacgcaggcc agctccgcgt cgaaggccgc agccaaggtc
     2341 gcggcggcag tcaaaagctc gcgatcggct tgagcccccg cgaggggagc cataattctc
     2401 gcccaggtca tgatcgaact cctttggcgc gcatttccac aggtgagggc ggacccatca
     2461 ttgatctttg tcaaccgata ggtcagtcaa tccaccttca ccgtaaggaa cgatctcgtg
     2521 gccaaagggc tgcatagggg cgcggcgccc cgcgcttggc agagaatgct ctcgggtcgg
     2581 aggctggatc tgcttgaccc gtcgcccatg gacatcgaga tcgaggacat cgcccatggc
     2641 ctggccaggg tcgctcgctg gaacggccag accgttggcg aacatgggtt ttccgtcgcc
     2701 cagcacagcc tggtggtcga ggagatcgcc gcccatatca agcccgacct cgaacccaaa
     2761 tggcgcctgg ccgccttgct gcacgacgcg tccgaatatg tgatcggcga catgatcagc
     2821 ccgttcaagg cggctctggg cgtcagctac aaggatttcg aggcgcggct ggaggatgcg
     2881 atccacatcc gcttcggcct gccggtaaag actccgccgg ccatcaagaa gctgatcaag
     2941 caggctgacc gggcctgcgc cttcttcgag gccacccagc tggccggctt tgaccatgcc
     3001 gagtcgctgt cgatcttcgg cgcgccgccg gtcggttatg agctgcacat caagccgcag
     3061 ccgccgttcg aggcccaggc tcactatgtt cagcgcttcc atctgctgtc ccgggccgcc
     3121 ggcttcgagt ccgcgcctaa cgtcgcgttc gagaccgaat gagcatatcc gtcgtcatcg
     3181 gcctttcggc cgtggtcgtc gccattcgcg acggcgaggc cgtggtgctg acggtccgac
     3241 cgcacgacgc gatcaccgac gtcgcctccc ccctgcccgg cctgccattc ggaccgttcg
     3301 acccggaaga gcaccggacc ttcgagctgg gcctgcgcgc cttcgtcacc cagcagaccc
     3361 gcttccagct gggctatgtc gagcagctct atacgttcgg cgacaagggc cgcgacgcgc
     3421 cgcgcgccga ggtgggcgcc ggcgccgcgc gggtcgtctc ggtcggctat ctgggcctga
     3481 ccccggacgc catcgacacc gacgcccccg acaccgtctg ggccccctgg tcgcgcttct
     3541 ttccgtggga agactggcgc catggccgtc ctgccttgct ggacgcggtc ctggctccgg
     3601 ccctgaaacg ctgggccgag ggtgacgcca gcaagtggtc cagggcccgc ctggccttcg
     3661 ctctggacga tgcgccctgg aacgaggagc gggtgcttga gcgctacgag ctgctgtacg
     3721 aggcgggtct ggcccccgaa gccgcccgcg accaggctcg ggccgatggc gacgatccgg
     3781 ctgagcccgt cgccctgtcg gcggcgctgg gcgagccgat gatctccgat caccgccgca
     3841 tcctggccac cggcctttcg cggctgcggg gcaagatcaa gtatcgcccc gtggtgtttg
     3901 aactgacgcc cgacgagttt accctctcga ccctgcagcg catcgtcgag gccatcgccg
     3961 gcgtgcccct gcacaagcag aatttccgcc gggtggtcga acgcgaggaa ctggtcgagg
     4021 gtctggggcg cctggacgcc gagaccggcg gccgtccggc cgagctgttc cggttccgcc
     4081 gcgagatcct ggcggcccgg ccggccacgg ggctcagcct gccgctgctg cgggagtagc
     4141 cgcttcaccc gcaggtcaat cgatcctcca ttctactttc gtcattcccg cccttgttgt
     4201 tcagaccggg gacatagcgg acacctgttc ggag
//
LOCUS       DAOMEO010000122         4221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2543_length_4221_cov_2.643063, whole genome shotgun sequence.
ACCESSION   DAOMEO010000122 DAOMEO010000000
VERSION     DAOMEO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_4221_cov_2.643063"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..473)
                     /locus_tag="PLH31_03885"
     CDS             complement(132..473)
                     /locus_tag="PLH31_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HQR88401.1"
                     /translation="MRPSLRLTFDRLDTGDWSGDGHAMSVTPNRKPRRRLTRVERWTL
                     DSLGGSLVAIGAVGVVLPGLPTTVFWIGAVVCFLKTRPLVVRPLLRTPVVGPAIIGFL
                     RWRPFGWGRRR"
     gene            complement(470..721)
                     /locus_tag="PLH31_03890"
     CDS             complement(470..721)
                     /locus_tag="PLH31_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88402.1"
                     /translation="MTDLISIAFRAMGWLLLVLGAALGIVAPAPVTTALWTAASRAFL
                     RGGDGAMVSRLLSATKFGPRIRRRLNRPAPRRLGGVCEA"
     gene            1024..1965
                     /gene="ubiA"
                     /locus_tag="PLH31_03895"
     CDS             1024..1965
                     /gene="ubiA"
                     /locus_tag="PLH31_03895"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQR88403.1"
                     /translation="MTAPMTTILPDAAATNWVDRHAPPALRPWLKLGRFDRPAGIWLL
                     MLPGWQGIALAGAEKGQWPNPFLLVAFFVGAALMRAAGCAFNDIVDRDFDAQVSRTAM
                     RPIPAGLISVKQAWAFVIGCCAVSFLILICLGWLAIGLGVLSLGLVAAYPFMKRITWW
                     PQAWLGLTFNWGVLLGYAAATGSLSWTAALLYASGIFWTLGYDTIYAVQDLEDDALAG
                     VKSSARKLGDRAQYGVAAFYAASFVLAVAAAWMGNLGALFLPLAALFAVHLSRQAAAL
                     RLDDPAGALKLFKSNTLAGLVLFLALVAGLWKPETRF"
     gene            complement(2092..2436)
                     /locus_tag="PLH31_03900"
     CDS             complement(2092..2436)
                     /locus_tag="PLH31_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HQR88404.1"
                     /translation="MRLSTLGLVLALTCVAGSASAASFDCRKARTADEKAICAERSLN
                     DKDVRMDVLYGVNKRTLAMGGRGALMDQQRDWLRDRRACGGNKACLNRAYDRRLGDLE
                     KSMERIYSNGPF"
     gene            2510..3442
                     /locus_tag="PLH31_03905"
     CDS             2510..3442
                     /locus_tag="PLH31_03905"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HQR88405.1"
                     /translation="MAVAPRVKPLDLFTPEEWAKVSARSSWRGIWMVAHAWGTILLAG
                     ALFVVFPNPLTYMLAVMIIGARQLGLAILMHEAAHGGLHPNLKVNDWLGEWLCAAPTG
                     ASLKSYRPYHLTHHKYAQQAEDPDLILSAPFPTTRQSLRRKIVRDLTGQTFFKQRFGP
                     LLGKLKGDQPKGAIFSGEIARQKPFLLWNLGLLVALSAVGLWWAWLALWIVPMATWFP
                     LVTRLRNIAEHALVAKDEPDPFRHARTTRANWLERALIAPYHVNFHAEHHMFMHTPCW
                     NLPLAHRLLEQKGLTGGMLTAPGYISVLRAVAHS"
     gene            3494..4063
                     /locus_tag="PLH31_03910"
     CDS             3494..4063
                     /locus_tag="PLH31_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88406.1"
                     /translation="MTLDPKFLDILKADGVKTFGLTIAFGLAFYGIHINVIPTPDKGW
                     MILLAVLWLIVGCLALMSLISASIKFLAPVERISRSFSRWKIVNEAKSYLPFMTDRER
                     SIISYLLERNWQTFTCTQDGGYAATLISTRIVIMKYQPGQMIHPDDVPFCIPPDIWKM
                     LQRHRNLFPPYVDDNDGAYPWRISWMVRS"
     gene            4105..>4221
                     /locus_tag="PLH31_03915"
     CDS             4105..>4221
                     /locus_tag="PLH31_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR88407.1"
                     /translation="MIDQTLEGRRAFILENTRVQAPPHAPELSLHLADEITPI"
BASE COUNT          712 a         1422 c         1331 g          756 t
ORIGIN      
        1 ccgggcgatg aagcccagca cctgcccgtt ggacagcgcc cgcttgcggc ccgccgcttc
       61 gtcctcgtcg tcgataaagt cagtcatgat gggcgcgtag ataggcggtt tggcgcgccg
      121 aggcgaccct gctagcgcct cctgccccag ccaaatggcc gccagcgcag gaagccgatg
      181 atcgccggtc ccaccaccgg cgtgcgcagc aggggccgca ccaccagggg ccgggtcttg
      241 aggaaacaga ccaccgcccc gatccagaat accgtggtgg gcaagcccgg caacaccacc
      301 ccgaccgcgc cgatggcgac cagcgagccg cccaggctgt ccaaagtcca gcgctcgacg
      361 cgggtcaggc ggcggcgggg cttgcggttg ggggtgacgc tcatggcgtg accatcgccg
      421 ctccagtcgc cggtgtcgag gcggtcgaag gtcagtcgca gggaaggcct cacgcctcgc
      481 agacgccgcc aaggcggcgt ggcgccggac ggttcaggcg gcgacggatg cggggtccaa
      541 acttggtggc cgacagcaag cgcgagacca tggccccatc gccgccgcgc aggaaggcgc
      601 gactggccgc cgtccacagg gccgtggtca ccggcgccgg ggccacgatg ccgagggccg
      661 cgcccaaaac cagcaagagc cagcccatgg cgcggaaggc gatggagatc agatccgtca
      721 tcggttcagg tccttggcca gatatcggcc agatatcggg gagagcggcc agctgggcga
      781 aagccgccct cccctcgccg tcagacagtc cagatacgcg gacggtccgg caacgatcgc
      841 ggcgggggtt gcgcaaagcg cctcccccaa accctcatct ggtggacaca ccctgcccgt
      901 gccggcagga ttaatccaat tgatcgtttt tcgtttcgag atcgagaaag cctatgatcg
      961 atagccgggc cccgtcggtc ggcgcctttt cggctccgca taagctcgct aagccttgcg
     1021 cccatgactg cccccatgac gaccatcctg cctgacgccg ccgcgaccaa ctgggtcgac
     1081 cgccatgcgc cgcctgcgct gcgcccctgg ctgaagctgg ggcgctttga ccggccggcg
     1141 gggatctggc tgctgatgct gcccggctgg cagggcatcg ccctggccgg ggccgagaag
     1201 ggccagtggc ccaatccgtt cctgctagtg gccttcttcg tcggcgcggc cctgatgcgg
     1261 gcggcaggct gcgcgttcaa cgacatcgtc gaccgcgact tcgacgccca ggtcagccgc
     1321 acggcgatgc ggccgatccc ggccgggctg atcagcgtca aacaggcctg ggccttcgtg
     1381 atcggctgct gcgcggtcag cttcctgatc ctgatctgcc tgggctggct ggccatcggg
     1441 ctgggcgtgc tgtcgctggg gctggtggcg gcctatccgt tcatgaagcg catcacctgg
     1501 tggccccagg cctggctggg cctgaccttc aactggggcg tccttctggg ctacgccgcc
     1561 gccaccggaa gcctcagctg gaccgccgcc ctgctctacg cgtcggggat tttctggacc
     1621 ctgggctatg acacgatcta cgccgtgcag gaccttgagg acgacgccct ggccggcgtg
     1681 aaatcctcgg cccgcaagct gggcgacagg gcgcagtacg gggtcgccgc cttctatgcc
     1741 gcctcgttcg tgctggccgt ggccgcggcg tggatgggga acctgggcgc cctgttcctg
     1801 ccgctggcgg cgctgttcgc ggtgcacctg tcacggcagg cggcggccct gcggctggat
     1861 gatccggccg gggcgttgaa gctgttcaag tccaacacgc tggcggggct ggtgctgttc
     1921 ctggcgctgg tggcggggct ctggaagccg gagacgcggt tctaagcgcg caccaagcct
     1981 tgtcatcccg ggcgaagcgc agcgaagacc cgggaccgcg acaagctcgg cgcttccggc
     2041 ggtcccggat cggcgcgcta cgcgcttgtc cgggatgaca gtgttagcgg atcagaacgg
     2101 cccgttgctg tagatccgct ccatgctctt ctccagatcg cccaaccgcc ggtcataggc
     2161 ccggttcagg caggccttgt tgcccccaca cgccctgcga tcccgcagcc agtcacgctg
     2221 ctgatccatc aacgccccgc gcccgcccat ggccagggtg cgcttgttga cgccgtacag
     2281 cacgtccatc cgcacgtcct tgtcgttcag cgaccgttcg gcgcagatcg ccttctcgtc
     2341 ggcggtgcgg gccttccggc agtcgaagct ggcggcgctg gccgagccag ccacacaagt
     2401 caaagccaga acgaggccga gggtcgaaag acgcatgggg atatctccga aagggcgatg
     2461 acgccgcgtc atccttcatg acccaagctg aatgtgcgat gatggttcca tggccgtggc
     2521 tcctcgcgtc aaacccctgg acctgttcac gcccgaggaa tgggcgaagg tttccgcgcg
     2581 ctcgtcctgg cgcggaatct ggatggtcgc ccacgcctgg gggacgatcc tgctggccgg
     2641 cgccctgttc gtggtcttcc ccaatcccct gacctacatg ctggcggtga tgatcatcgg
     2701 cgcgcggcag ctgggcctgg cgatcctgat gcacgaggcg gcccatggcg gcctgcatcc
     2761 gaacctgaag gtcaatgact ggctgggcga gtggctgtgc gccgccccga ccggcgcgtc
     2821 gctgaagagc tatcgcccct atcacctgac ccatcacaaa tacgcccagc aggccgagga
     2881 ccccgacctg atcctgtcgg cgcctttccc gaccacgcgg cagtcgttgc gccgcaagat
     2941 cgtccgcgac ctgacgggcc agaccttctt caagcagcgg ttcgggcccc tactgggcaa
     3001 gctcaaaggg gatcagccga aaggcgcgat cttcagcggc gagatcgccc gccagaagcc
     3061 gttcctgctg tggaatctgg gcctgctcgt cgccctgtcg gcggtcggcc tttggtgggc
     3121 gtggctcgcg ctatggatcg tgcctatggc gacctggttc ccgctggtca cccgcctgcg
     3181 caacatcgcc gaacacgccc tggtcgccaa ggacgagccc gaccccttcc gccacgcccg
     3241 caccacccgc gccaactggc tggaacgcgc cctgatcgcg ccctaccacg tcaatttcca
     3301 cgccgagcac cacatgttca tgcacacccc gtgctggaac ctgcccctgg cgcaccggct
     3361 gctggagcag aagggcctga cgggcgggat gctgacggcg ccggggtaca tctcggtcct
     3421 gcgcgcggtt gcgcacagct agtcgtttag ggtcggagtt atcgaatcga tcgaacggcg
     3481 ttatcaattc cgcatgacgc tcgatcctaa attcctggac atactgaagg cagacggcgt
     3541 caaaaccttc ggcctaacga tagcttttgg cctcgccttc tacggcatcc acatcaacgt
     3601 tattccgacc ccagataaag ggtggatgat ccttctcgcg gtgctgtggt tgattgtcgg
     3661 ctgcctcgca ctaatgagct tgatcagtgc gtcaattaag ttcttggctc ccgtcgagcg
     3721 aattagtcgt tcattcagcc gatggaaaat tgtaaatgag gcaaaatctt atttaccatt
     3781 catgactgac agagaaagga gcatcataag ctatcttctg gaacgcaatt ggcaaacttt
     3841 cacttgcact caagatggcg gatacgctgc tacgctcatt tctacacgaa tcgtcattat
     3901 gaagtatcag ccaggacaga tgatccaccc agatgacgtg ccgttctgta tcccgccaga
     3961 catctggaag atgcttcaga gacaccgaaa tctcttccct ccttacgtcg atgataacga
     4021 cggcgcatat ccttggcgca tttcatggat ggttcgctct tagcgctgct cgcgttggcc
     4081 gctccatggt gctctaagcg ccgcatgatc gaccagacgc tggaaggccg ccgcgccttc
     4141 attctcgaaa atacccgcgt ccaggcgccg ccccatgcgc ccgagctgtc gttgcatctg
     4201 gccgacgaga tcaccccgat c
//
LOCUS       DAOMEO010000123         4210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2546_length_4210_cov_2.236342, whole genome shotgun sequence.
ACCESSION   DAOMEO010000123 DAOMEO010000000
VERSION     DAOMEO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_4210_cov_2.236342"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLH31_03920"
     CDS             <1..836
                     /locus_tag="PLH31_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR88408.1"
                     /translation="AALFGAIMTLCIGTSFAKQLFPLVGAQGTTAYRVGFSALLLLAL
                     WRPWRHPLSRADLTAVALYGAVMGLMNLCFYMSLRTIPLGLAIAIEFTGPLALALLHS
                     RRLIHFVWIGLAAAGLLLLLPLKPGADALDPVGVAFAGAAAVLWALYIVLGKRTGHMH
                     AGRTVALGMSTAALIVAPIGLVSAGAELFHPRVLMLGLVVAVLSSAIPYSLEMIALKG
                     IPKRSFGVMLSAEPAVGALAGLLMLGERLAPLQWLAIGCIIAASAGTILTTPAEVEPA
                     P"
     gene            complement(1031..1813)
                     /gene="cysQ"
                     /locus_tag="PLH31_03925"
     CDS             complement(1031..1813)
                     /gene="cysQ"
                     /locus_tag="PLH31_03925"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQR88409.1"
                     /translation="MTVSDLPLADWGRALAGICEEAASIILPFWRTELSVTRKADSSP
                     VTEADQAGERLILQRLAEVFPDVPTVSEEHASEFGAPEDIGPRFFLLDPIDGTKAFVR
                     GDPSFTVNIGLIENGVPVAGAIVSPVTGETWFTTSDGVLKRLQAGGAETGVEVRAWPR
                     GEAVALISHTMTEARAAELAVEYGFDLRTPMDSSIKLCRIAEGAADIYPRHGPTMEWD
                     TAAGHAILVAAGGRFTKPDGGAFVYGKADQGFRNGWFVARGG"
     gene            complement(1814..1999)
                     /locus_tag="PLH31_03930"
     CDS             complement(1814..1999)
                     /locus_tag="PLH31_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HQR88410.1"
                     /translation="MDPFLEPGVLVRHPDQPDWGLGQVQSVIGHRVTVNFEDAGKQTI
                     DAQRVTLVFVGDDPRGS"
     gene            2066..2737
                     /gene="chpT"
                     /locus_tag="PLH31_03935"
     CDS             2066..2737
                     /gene="chpT"
                     /locus_tag="PLH31_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase ChpT"
                     /protein_id="HQR88411.1"
                     /translation="MTDTPPEPAPTTTVDVNGPDFAAMLAARLCHDFISPASAIVSGL
                     DLLEDPSAQDMREDAMSLIASSARKLADLLQFCRVAFGASASAEAFDSRELEKLAQGV
                     FDHVRPTMVWSIDSQPINKAAARALLNIAQLSASALPAGGVATLKGVTAEGRFLITAD
                     SVGPRARLRPEVLAGLRGEALTEGLGGPWVQAAYLHALVRAAGGQVAVEAGEDRISLA
                     CWVPA"
     gene            2820..>3128
                     /locus_tag="PLH31_03940"
     CDS             2820..>3128
                     /locus_tag="PLH31_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88412.1"
                     /translation="MEHAVKTCLVVDDSRVIRKVARRVLEDIGFEIAEAADGMEALAW
                     CRAAMPDAVLLDWNMPVMNGIEFLRQLRNEPGGDTPTVVFCTVENDVDHIRAALEAGA
                     N"
     assembly_gap    3129..3138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3139..3208
                     /locus_tag="PLH31_03945"
     CDS             <3139..3208
                     /locus_tag="PLH31_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88413.1"
                     /translation="EYIMKPFDGEIIAAKFAEAGLL"
     gene            3205..4038
                     /locus_tag="PLH31_03950"
     CDS             3205..4038
                     /locus_tag="PLH31_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQR88414.1"
                     /translation="MTPEDMDLLAALGRARAGLAVDVDKTYVVESRLAPLARREGFDS
                     IEALIAALRAKREERLIWAVVEALTFNETSFFRDREVFTHLRDTVLPTLSKARRDQPI
                     RIWSAACSTGQEVYSLAMAAGESREVEPGAKFEFFGSDLSERCLEKAQSGLYTQFEIQ
                     RGLPITQLVKHFANQDDTWEISPRIRQSVRWKRINLLSDLSSLGRFDVILLRHVLAGI
                     DASLHGRLIESLAARLADDGVLVLSPDDLLTGAAATLFEASDQAGVFARRPADQAEAA
                     A"
     gene            complement(4120..>4210)
                     /locus_tag="PLH31_03955"
     CDS             complement(4120..>4210)
                     /locus_tag="PLH31_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HQR88415.1"
                     /translation="AACNTVEGAGKDVSSAGKAVTETAQDAKN"
BASE COUNT          616 a         1477 c         1403 g          704 t
ORIGIN      
        1 acgccgccct gttcggcgcg atcatgacgc tgtgcatcgg cacgtccttt gccaagcagc
       61 tgttcccgct ggtcggcgcc cagggcacga ccgcctatcg cgtaggcttt tcggccctgc
      121 tgctgctggc cctctggcgg ccgtggcgac acccgctcag ccgcgctgat ctgacggccg
      181 tggcgctgta cggcgcggtg atggggctga tgaacctctg cttctacatg tccctgcgga
      241 ccatcccgct gggtctggcc atcgccattg aatttaccgg gccgctggcc ctggccctgc
      301 tgcattcgcg ccggctgatc catttcgtct ggatcggact ggccgccgcg ggcctgttgc
      361 tgctcctgcc gctgaaaccc ggcgccgacg ccctcgaccc cgtcggcgtg gctttcgcgg
      421 gcgcggcggc ggtgctgtgg gcgctctata tcgtgctggg caagcgcacc ggccatatgc
      481 acgctggccg caccgtggct ctgggcatga gcacggcggc cctgatcgtg gcgccgatag
      541 gcctggtctc ggccggcgcc gagctcttcc atccccgcgt gttgatgctg ggcctggtgg
      601 tggcggtgct gtccagcgcc atcccctatt cgctggagat gatcgccctg aagggcatcc
      661 ccaagcgcag tttcggcgtg atgctcagcg ccgagccggc ggtgggcgcc ctggcaggcc
      721 tgctgatgct gggcgagcgc ctggcgccgc tgcagtggct ggccatcggc tgcatcatcg
      781 ccgcctcggc cggaacgatc ctgacgacgc cggctgaggt cgagccggcg ccctaaagtc
      841 ctctccctga aggccgggcg tttgcatacg gcgctgtgag ggaaaaactg tcatcccgga
      901 caagcccggc gaaagccggg cgcagatccg ggaccgccgg aagcgttgag cccaggcgtc
      961 ggtcccggat cggcgcgctt cgcgcttgtc cgtccgggat gacatccgtt ttggtcagag
     1021 gtgatagccc ttaaccgccc cgcgccacga accagccgtt gcggaagccc tgatcggcct
     1081 tcccgtagac gaacgccccg ccgtccggct tggtgaacct tccgccggcg gcaaccagga
     1141 tcgcatggcc ggcggccgtg tcccattcca tggtcggacc atggcgcgga tagatatcgg
     1201 ccgcgccctc ggcgatgcgg cacagcttga tcgagctgtc catcggagtg cggaggtcaa
     1261 agccatattc gacggccagt tcggccgcgc gggcctctgt catggtgtgg ctgatcaggg
     1321 ccacggcctc gcccctgggc caggcgcgga cctcgacccc cgtctccgcg ccgcccgcct
     1381 gcaggcgctt gagcacgccg tccgaagtgg tgaaccaggt ctcgcccgtc accggggaca
     1441 ctatggcgcc agcgaccggg acaccgtttt cgatcaggcc gatattgacg gtgaagctgg
     1501 gatcgccgcg cacgaaggcc ttggtgccgt cgatcggatc gagcaggaaa aagcgcggcc
     1561 cgatatcctc gggcgcgccg aattcgctgg catgctcctc ggagacggtc ggcacgtccg
     1621 ggaagacctc ggccagtcgc tgcaggatca ggcgctcgcc ggcctgatcg gcctcggtca
     1681 ccgggctgga gtcggctttc cgggtgacgc tgagctcggt gcgccagaac ggcaggatga
     1741 tgctcgcggc ctcctcgcag atcccggcga gggcgcggcc ccagtctgcg agcggcaggt
     1801 cggaaacagt cattcaggac cctctgggat cgtcgccgac gaagacgagc gtcacgcgct
     1861 gggcgtcgat cgtctgcttg ccggcgtctt cgaagttgac ggtgacgcga tggccgatca
     1921 ccgattgcac ctggcccagg ccccagtcgg gctggtcggg atggcgcacg aggacgccag
     1981 gttcaagaaa cggatccata acgcgcccct gatagggccc acgccccgtt tgccaaagcc
     2041 tcaaatcacg ccagagtctg cgaccatgac cgacacgccc cccgagcccg ccccgaccac
     2101 gaccgttgac gtcaacggtc ccgacttcgc cgcgatgctg gcggcgcgcc tgtgtcacga
     2161 cttcatcagc ccggccagcg ccatcgtgtc ggggctggac ctgctggagg atccctccgc
     2221 ccaggacatg cgcgaggacg ccatgagcct gatcgcctcg tcggcccgca agctggccga
     2281 cctgctgcag ttctgccggg tcgccttcgg cgcctcggcc tcggccgagg ccttcgactc
     2341 gcgcgagctg gaaaagctgg cccagggcgt ctttgaccat gtgcggccca ccatggtgtg
     2401 gtccatcgac tcccagccga tcaacaaggc cgccgcccgc gccctgctca acatcgccca
     2461 gctgtcggct agcgccctgc cggccggcgg cgtcgccacc ctgaagggcg tcaccgccga
     2521 gggccggttc ctgatcaccg ccgattccgt cggcccccgc gcccgcctgc ggcccgaagt
     2581 gctggccggc ctgcgcggcg aggccctgac cgaaggtctg ggcggaccct gggtgcaggc
     2641 ggcctatttg cacgccctgg tgcgcgccgc gggcggccag gtcgcggtag aggcgggcga
     2701 ggatcgcatc agcctggcct gctgggtccc ggcctgaccc tgatcgggga cgaagggcgc
     2761 ctttcggggc ttatgctcac cgctccttaa cctcggcgcc cgacgatggg cgcttgggat
     2821 tggagcatgc cgtgaagacc tgtctggtgg tcgacgacag ccgggtgatc cgcaaggtcg
     2881 cccgccgagt ccttgaggac atcggcttcg agatcgccga ggccgccgat ggcatggagg
     2941 cgctcgcgtg gtgtcgcgcc gccatgcccg atgctgtcct gctcgactgg aacatgcccg
     3001 taatgaacgg catcgagttc ctgcgccagc tgcgcaacga accgggcggc gacacgccga
     3061 ccgtggtgtt ctgcaccgtc gagaacgacg tcgaccatat ccgcgcagcc ctcgaggccg
     3121 gcgccaacnn nnnnnnnncg aatacatcat gaagcctttc gacggcgaga tcatcgcggc
     3181 gaagttcgcc gaggcgggcc tgctgtgacc cccgaggaca tggaccttct ggccgccctt
     3241 ggccgcgccc gcgccggcct ggcggtcgac gtcgacaaga cctacgtcgt cgaaagccgc
     3301 ctggcgccgc tggctcgccg cgagggcttc gactcgatcg aggccctgat cgccgccctg
     3361 cgcgccaagc gcgaggaacg cctgatctgg gcggtcgtcg aggccctgac cttcaacgag
     3421 accagcttct tccgtgaccg cgaggtcttt actcacctgc gcgacacggt tctgccgacc
     3481 ctgtcaaagg cgcgccgcga ccagccgatc cgcatctgga gcgcggcctg ctcgacgggc
     3541 caggaagtct actccctggc catggccgca ggcgaaagcc gcgaggtcga gccgggcgcg
     3601 aagtttgagt tctttggctc cgacctgtcg gaacgctgcc tggaaaaggc ccagagcggc
     3661 ctctacaccc agttcgagat ccagcgcggc ctgccgatca cccagttggt caaacacttc
     3721 gccaatcagg atgacacctg ggagatctcg ccgcgcatcc gccagtcggt gcgctggaag
     3781 cggatcaacc tgctcagcga cctgtcctct ctggggcggt tcgacgtcat cctgctgcgt
     3841 catgtgctgg ccggcatcga cgcctcgctg cacggccgac tgatcgagag cctggccgcg
     3901 cgcctggccg atgacggggt gctggtgctg agcccggacg accttttgac cggcgccgcc
     3961 gccaccctgt tcgaagcatc agaccaggct ggcgtcttcg cacgtcgccc cgccgaccag
     4021 gccgaagcgg ccgcctgaca gccttgacgc gacgcacgaa aaagcccgcc gatcccagag
     4081 gaccggcggg ctttcctgtt ctggcctgaa ggggctgaac tagttcttgg cgtcctgggc
     4141 ggtctcggtg acagccttgc cagccgacga gacatccttg ccggcgcctt cgacggtgtt
     4201 gcaggcggcg
//
LOCUS       DAOMEO010000124         4188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2555_length_4188_cov_2.890152, whole genome shotgun sequence.
ACCESSION   DAOMEO010000124 DAOMEO010000000
VERSION     DAOMEO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_4188_cov_2.890152"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>870
                     /locus_tag="PLH31_03960"
     CDS             <1..>870
                     /locus_tag="PLH31_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR88416.1"
                     /translation="AITDARTEAFITDLMSRMSLEEKVGQTIQGDGGSITPDDLRKYP
                     LGSVLVGGNSAPDGNDRATPQRWVEWTRDFRAAALEKRADRQSIPIIFGVDAVHGHNN
                     VVGATLFPHNIGLGAARDPDLIRRIGEVTALEMAASGADWTFGPTVAVPRDERWGRAY
                     EGYAEDPEVVKAYAGPMTLGLQGALKAGQRLEADRVVGSAKHFLADGGTEGGKDQGDA
                     LISEQDLIRLHAQGYPPAIDAGILTVMVSFSSWNGVKITGNKSLQTDVLKGKMGFEGF
                     VVGDWNAHGQVTG"
     assembly_gap    871..880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <881..2336
                     /locus_tag="PLH31_03965"
     CDS             <881..2336
                     /locus_tag="PLH31_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HQR88417.1"
                     /translation="ARPSRLVGRRGGVSGAGLPDSWKGLFDNTLIQVKDGTIPMARLD
                     DAVRRILRVKVKAGLFENKRPLEGKLELLGAPEHRAVAREAVRKSLVLLKNQGVLPIK
                     SSARVLVTGDGADDIGKQAGGWTLTWQGTGNKNSDFPNGQSIYAGIAEAVKAGGGQAE
                     LAKDGAFKDKPDVAVVVFGENPYAEFQGDITSLEYQPGDKSDLALIKKLKAQGIPVVA
                     VFLSGRPLWTNPEINAADAFVAAWLPGSEGGGVADVLVGDKAGKPRHDFKGTLSFSWP
                     KYANQVPLNHGDANYDPQFAYGYGLSYAEPAQVAQLSEDPGMVSAAVNVDRYFVAGRA
                     PAPWTLNVGGALSVKPIDAGAQENARLAEWSGEGAGSLNVIGQPVDLSRQTTGDMAVA
                     IRYRVDAAPQKPVTLAIGCGESCGAEVDVTSTFSSAKIGEWRTAKIKLSCFMAKGANM
                     EHVSSPFALSTTGRFALAFTDIRLASNEGDAVCP"
     gene            2356..3786
                     /gene="pyk"
                     /locus_tag="PLH31_03970"
     CDS             2356..3786
                     /gene="pyk"
                     /locus_tag="PLH31_03970"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQR88418.1"
                     /translation="MIRARRSRIVATIGPASSSPEMIVTLAKAGADVFRLNFSHGAHE
                     THAAVYAAIRAAEPIVGRPLGILADLQGPKLRVGKFANGPVQLQAGQAFRFDSDPTPG
                     DETRVCLPHPEILVAMRPGATLLLDDGKLRMTVTEAGPGYADTKVVNGGKLSERKGVA
                     VPDVVIPMSPLTPKDREDLAFALRLGVDWVALSFVQAPEDMAELRRIVEGRAAVLAKI
                     EKPQALERLGAILDLCDGVMVARGDLGVEMAPEEVPVAQKIILRAARERGIPVIVATQ
                     MLESMTSSPTPTRAEASDVANAVYEGADAVMLSAESAAGDYPEEAVSMMNRIIERVER
                     DPRWPELMQAEQPHQDDDADVLVVAAAGAAKAGSTKCLVAFTTTGATARRLARERPLQ
                     PVLALSPQIDAVRRMTLVWGIEPRVSAQPDSLEVVTTDAASRALELGLVAPGERLLIV
                     AGTPFGAPGAANLLRLAHAPLPIRKR"
     gene            complement(3783..>4188)
                     /locus_tag="PLH31_03975"
     CDS             complement(3783..>4188)
                     /locus_tag="PLH31_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR88419.1"
                     /translation="RIGDSVMHDLSARLPTLRSLIDRHNEALFGHAIQSAACNALHAV
                     EARFCRWLLSCDDRIDTNMVQLTQEVLADMLGVQRTTVTAVAGSLQTKGLIRYRRGVV
                     DILDRAGLEAMACECYGAVRRGYERLLPASFG"
BASE COUNT          701 a         1388 c         1438 g          651 t
ORIGIN      
        1 gcgccattac cgacgccagg accgaggcgt tcatcaccga tctgatgagc aggatgagcc
       61 tggaagagaa ggtcggtcag accatccagg gcgacggcgg atcgattacg ccggacgacc
      121 tgcgcaagta ccccctgggc tcggtgctgg tgggcggcaa cagcgcgccg gacggcaatg
      181 accgggccac gccccagcgc tgggtggaat ggacccgcga cttccgcgcc gctgctctgg
      241 agaagcgcgc cgatcgtcaa agcatcccga tcatcttcgg cgtcgacgcc gtgcacggcc
      301 ataacaacgt cgtcggggcc acgctctttc cacacaatat cggcctgggc gcggcccgtg
      361 atcccgacct gatccgtcgc atcggcgagg tcacggcgct ggaaatggcc gcctcgggcg
      421 ccgactggac ctttggtccc accgtggccg tgccgcgcga cgagcgctgg ggccgcgcct
      481 atgagggcta tgcggaagat ccggaggtcg tgaaagccta tgccgggccc atgacgcttg
      541 gtctgcaagg cgccttgaag gccggccagc ggctggaggc cgaccgcgtg gtgggttcgg
      601 ccaagcactt cctcgccgac ggcggcaccg agggcggcaa ggaccagggc gacgccctga
      661 tctccgagca ggacctgatc cgcctgcatg cacagggcta tccgcccgcc atcgacgccg
      721 gcatcctgac ggtgatggtc tcgttctcca gctggaacgg ggtgaagatc accggcaaca
      781 agagcctgca gaccgacgtc ctgaagggca agatgggctt cgagggcttt gtggtcggtg
      841 actggaacgc ccatggccag gtgacgggct nnnnnnnnnn tgcgcggcct tctaggctgg
      901 tcggtcggcg tggcggtgta tctggtgctg gcctccccga cagctggaag ggcctgttcg
      961 acaataccct gatccaggtc aaggacggca cgatcccgat ggcgcgcctg gacgacgccg
     1021 tacgccggat cctgcgggtc aaggtcaagg cgggcctgtt cgagaacaag cgccctctgg
     1081 aaggcaagct tgagctgctg ggcgcgcccg agcaccgggc cgtggcgcgt gaggccgttc
     1141 gcaagtctct cgttctgctg aagaaccagg gcgtcctgcc gatcaagagc tcggccaggg
     1201 tgctggtgac cggcgacggc gccgacgaca tcggcaagca ggccggcggc tggaccctga
     1261 cctggcaggg cacgggcaac aagaacagcg acttcccgaa cggccagtcg atctatgccg
     1321 gcatcgccga ggcggtgaag gccggcggcg gccaggccga gctggccaag gacggcgcgt
     1381 tcaaggacaa gcctgatgtt gcggtcgtgg tgttcggcga aaatccttat gccgagttcc
     1441 agggcgacat caccagcctt gagtatcagc cgggcgacaa gtccgacctg gccctgatca
     1501 agaaactcaa ggcccagggc atccccgtgg tcgcggtgtt cctgagcggc cgcccgctgt
     1561 ggaccaaccc cgaaatcaac gccgccgacg ccttcgtcgc cgcctggctg ccgggcagtg
     1621 aaggcggcgg ggtggctgac gtgctggtcg gtgacaaggc cggcaagccg cgccacgact
     1681 tcaagggaac actgtcgttc tcatggccaa agtacgccaa tcaggttccg ctcaaccacg
     1741 gcgacgcgaa ctatgatccg cagttcgcct atggctatgg cctgagctac gccgagcctg
     1801 cccaggtcgc gcagctgtcg gaagatccgg gcatggtctc ggcggcggtc aatgtcgatc
     1861 gctacttcgt cgctggccgc gctccggccc cctggacgtt gaacgtcggc ggcgccctgt
     1921 cggtgaagcc catcgacgcc ggcgcccagg agaatgcccg cctggccgaa tggagcgggg
     1981 agggcgcggg ctcgctgaac gtcattggcc agccggtcga cctttcacgc cagacgaccg
     2041 gcgacatggc ggtcgccatt cgctaccggg tcgacgccgc cccacagaaa ccggttaccc
     2101 tggcgatcgg ctgtggcgaa tcctgcggtg cggaggtaga cgtaacgtca actttctcaa
     2161 gcgcgaaaat cggcgaatgg cggacggcca agatcaaact ttcctgtttc atggccaagg
     2221 gcgcgaacat ggagcatgtt tcgtccccct tcgccctgtc gacgaccgga cggtttgctc
     2281 tggcattcac cgacatccgg ctcgcgtcca acgagggcga cgccgtctgc ccgtaatcac
     2341 acaaggagcg gccgcatgat ccgcgcgcgt cgttcccgta ttgtcgccac cattgggccg
     2401 gccagcagtt cgccggagat gatcgtcacc ctggccaagg ccggggctga cgtgttccgg
     2461 ctgaacttca gccacggcgc acatgagacc catgcggcgg tctatgccgc gatccgggcg
     2521 gccgagccga tcgttggccg gccgctgggc attctggccg acctgcaggg ccccaagctt
     2581 cgggtcggca agttcgccaa tgggccggtt cagcttcagg cagggcaggc gttccgtttc
     2641 gactccgacc cgacgccggg cgatgagacg cgtgtctgcc tgccgcatcc agaaatcctg
     2701 gtggccatgc gccccggcgc gaccctgctg ctggatgacg gcaagctgcg gatgacggtg
     2761 accgaggccg gccccggcta tgccgacacc aaggtcgtca atggcggcaa gctgtcggaa
     2821 cgcaagggcg tggcggtgcc ggatgtcgtc atcccgatgt cgcccctgac gccgaaggac
     2881 cgcgaggacc tggcctttgc cctgcgcctc ggcgtcgact gggtcgccct gtcgttcgtg
     2941 caggcgccgg aagacatggc cgaactgcgc cgcatcgtcg aaggccgcgc cgccgtgctg
     3001 gccaagatcg agaagccgca ggcgctggaa cgcctgggcg caatcctgga cctgtgcgac
     3061 ggcgtgatgg tcgcgcgcgg cgacctgggc gtcgagatgg cccccgaaga ggtgcctgtc
     3121 gcccagaaga tcatcctgcg ggcggcgcgg gagcgcggca tcccggtgat cgtggccacc
     3181 cagatgctgg aatccatgac cagctcgccc accccgaccc gcgccgaagc ctcggatgtg
     3241 gccaacgccg tctatgaggg cgctgatgcg gtgatgctgt cggccgagtc cgccgccggc
     3301 gactatcccg aggaagcggt ctcgatgatg aaccgcatca tcgagcgggt ggagcgtgat
     3361 ccgcgctggc ccgagctgat gcaggccgag cagccccatc aggatgacga cgccgacgtg
     3421 ctggtggtcg ccgccgccgg cgccgccaag gccggatcga ccaagtgcct ggtggccttc
     3481 accaccaccg gcgccacggc gcggcgtctg gcgcgcgagc gtcccctgca gccggtgctg
     3541 gccctgtcac cgcaaatcga cgccgttcgc cgcatgacgc tggtctgggg tatcgagccc
     3601 cgcgtcagcg cccagcccga cagccttgag gtcgtgacca ccgacgcggc cagccgcgcg
     3661 ctggaactgg ggctggtggc tccgggcgag cgtctgctga tcgtggcggg gacgcctttc
     3721 ggggcgccgg gcgcggccaa cctgctgcgc ctggcccacg cgccgctgcc gatccgcaag
     3781 cgctagccaa aggacgccgg cagaagtcgc tcgtatccgc gtcgaaccgc gccatagcat
     3841 tcgcaggcca tggcctcgag acctgcgcga tccagaatat cgaccacgcc acggcgatag
     3901 cggatcaggc ccttggtctg cagcgaaccg gcgacggcgg tgaccgtggt gcgctggacg
     3961 cccagcatgt cggccaggac ctcctgggtc agctggacca tgttggtgtc gatccggtcg
     4021 tcgcacgata acaaccagcg gcagaatcgc gcctcgaccg cgtggagcgc gttgcaggcg
     4081 gccgattgta tggcatgccc aaacagcgct tcattgtggc gatcaatcag gcttcgcagg
     4141 gtgggcaggc gcgcgctgag atcgtgcatc acgctgtcgc cgatgcgc
//
LOCUS       DAOMEO010000125         4186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2558_length_4186_cov_2.746308, whole genome shotgun sequence.
ACCESSION   DAOMEO010000125 DAOMEO010000000
VERSION     DAOMEO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_4186_cov_2.746308"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="PLH31_03980"
     CDS             <1..1688
                     /locus_tag="PLH31_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate-binding protein"
                     /protein_id="HQR88420.1"
                     /translation="DLVESVVNHQFEPIIAAEWRSARLDFGSLPLLLKSLGQGPGRAG
                     LTLSNEVLDERTLRKLSQQDDVIARCKADRSALLRLWDVCQTPDFRKTTDDDHQRMVR
                     TLFDDLTTGKKRLPEDWINSQFKALDRTDGEIDSLAARLSGVRTLSYIANRPDWLANP
                     AHWQGVTRQLEDRISDTLHEKLMARFIDRRTSVLMRQLGEREVMLAGVSPDGAVTVEG
                     HVVGHLAGVTFTPEKGASALEEKALRNAAVRAVGPEIARRLGALAAEPDDAFALNPDS
                     AILWRGEIAGVVRPGLPLLSPRARLLGELGPAAVRERAERRLDAFLATEADRHLAPLR
                     KLERALAGGELRGLPRGLAHRLLEAAGVLDKRPLDPELKALSQAERRTLKSLGVRIGA
                     FSLYLPAMLRPEAIACARALSPGGWRGDVSRLAALPSPAPSARELAASGLRAVGGFTA
                     PVEMLEKLDDLLRAEQRPGGVVLTDAALETLGWTAAQASAVLRALDYTPAIRPKANAP
                     IIWKRRGAQRLDPKPVAARPDSPFAALARLTEPPPRRKKPRRRKPAAQKAAAS"
     gene            1685..1966
                     /locus_tag="PLH31_03985"
     CDS             1685..1966
                     /locus_tag="PLH31_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQR88421.1"
                     /translation="MSETDDACRADVWLWRARFFKTRSMACRFVEEGRIRLTRAGIES
                     RIDKPSRALKGGDVLVFGLGGRVIAVRVLGAGERRGPASEAREMYEVVG"
     gene            complement(2087..4171)
                     /locus_tag="PLH31_03990"
     CDS             complement(2087..4171)
                     /locus_tag="PLH31_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR88422.1"
                     /translation="MIKSTVEVRRSPASETGASRLPDSRAVAFSQFVVQLPFAAALLD
                     RDLRLLAVSREWTAHGLAPDLRPGVDARRALVAAEDVLALMKCAESGDAFSRYLPAPD
                     ADGVQRIWRTEFSACRDEGETPYAVMVTARDVTGYAEATLRAERDQQRLRMALELDGL
                     LVREYDLVTRELVFSGATPDLEKWCRFKTDPLEIIHRDDRDRVAALVATRKPGETGVY
                     EFRLNRDDGVETWVRSASKVLVGAHGRPERLVNLFKDITDRRRQTEAVETLAFKDTLT
                     DLPNRALFQHRFQEAVSASELLGEMFGLIMIDVDHFKDINDTLGHDAGDALLKRLAEM
                     LRHAFRADDTVARLGGDEFAVILRGLHGEADMLRPIQTLQELLRRPIEHGGRSFTCSA
                     SIGAALHGDPDADPSHMVKNADIALYRAKDAGRNRSVMFQPSMRSELEQRIELLRDVR
                     AGIAAGEFVLFYQPVVDVRVGGVTGFEALMRWAHPEQGILAPARFMAAFEDQDLSLKL
                     GDVTFEAALQQMRTWIDAGVEFGRVAVNISAAQFRSGRLAEEIQERLARWRVPCDRLT
                     IEVTENVYMGWGSDLVSETVKRLHDAGVMIALDDFGTGYASLANLRQFPIDRLKIDKS
                     FVQNAEDEAIVKAVITLGSSMGMKVVAEGVEDQAQLDKLIGYGCDQVQGYHFGRPMPA
                     DAVPGFLAGFAG"
BASE COUNT          647 a         1498 c         1433 g          608 t
ORIGIN      
        1 aggacctggt cgagagcgtg gtcaaccacc agttcgagcc gatcatcgcg gccgagtggc
       61 gcagcgcccg gctggatttc ggctctctgc cgctactgct caagtcactg ggtcaggggc
      121 cgggccgtgc gggcctgacc ctgtcgaacg aggtgctgga cgagcgcacc ctgcgcaagc
      181 tgtcccagca ggacgatgtc atcgcccgct gcaaggccga ccgctctgcc ctgctgcgcc
      241 tgtgggacgt gtgccagacg cccgacttcc gcaagaccac cgacgatgac caccagcgca
      301 tggtcaggac gctgttcgac gacctgacca cgggcaagaa gcggctaccg gaggactgga
      361 tcaatagcca gttcaaggcc ctggaccgca ccgacggcga gatcgacagc ttggccgcgc
      421 ggctgtcggg cgtgcgcacc ctgtcctata tcgccaaccg ccccgactgg ctggccaatc
      481 ccgcccactg gcagggcgtg acccgtcagt tggaagaccg catcagcgac accctgcacg
      541 aaaagctgat ggcccggttc atcgaccgcc gcaccagcgt gctgatgcgc cagctgggcg
      601 aacgcgaagt gatgctggcc ggcgtcagtc ccgacggcgc ggtgacggtc gagggccatg
      661 tggtcggcca tctggccggc gtcaccttca cccccgaaaa gggcgccagc gcgctggagg
      721 aaaaggccct gcgcaacgcg gccgtccgcg cggtcggtcc cgagatcgca cgtcggctgg
      781 gcgccctggc cgccgaaccg gatgacgcct ttgccctcaa tcccgacagc gccatcctgt
      841 ggcgcggcga gatcgccggc gtggtccggc caggcctgcc cctgctgtcg ccccgcgccc
      901 gcctgctggg cgagctgggg cccgccgccg tgcgcgagcg ggccgagcgg cgcctggacg
      961 cctttctggc taccgaagcc gaccggcatc tggccccgct gcgcaagctg gaacgggccc
     1021 tggccggcgg cgagcttcgc ggcctgccgc gcggcctggc ccaccgcctg ctggaagccg
     1081 ccggcgtgct ggacaagcgc cccctcgacc ccgagctgaa ggccctcagc caggctgagc
     1141 gccgcaccct gaaatcgctg ggcgtccgca tcggcgcctt cagtctctat ctgccggcca
     1201 tgctgcgccc cgaggccatc gcctgcgccc gcgccctgag ccccggcggc tggcgcggcg
     1261 acgtgagcag actggccgcc ctgccctcgc ccgccccctc ggcgcgagaa ctggcggcca
     1321 gcggtctgcg cgcggtcggc ggtttcacgg cgccggtcga gatgctggaa aagctcgacg
     1381 acctgctccg cgccgagcag cggccgggcg gggtggtgct gaccgacgcg gcgctggaaa
     1441 ccctcggctg gaccgccgcc caggcctcgg ccgtgctgcg ggcgctggac tacacgccgg
     1501 ccatccgccc caaggccaac gccccgatca tctggaagcg tcgcggggcg cagcgcctgg
     1561 atcccaagcc cgtcgcggcc cgtccggatt cgcccttcgc cgccctggcc aggctcaccg
     1621 aaccgccgcc gcgtcgcaag aagccgcgtc ggcgcaagcc cgccgcgcag aaggccgccg
     1681 cctcgtgagc gagaccgacg acgcctgccg cgccgatgtg tggctgtggc gcgcgcggtt
     1741 cttcaagacc cggtcgatgg cttgccgttt tgtcgaggaa ggtcgaatcc ggctgacccg
     1801 cgccgggatc gaaagccgga tcgacaagcc ctcgcgggcc ctgaagggcg gagatgtgct
     1861 ggtgtttggc ctcggcggcc gggtgatcgc ggtgcgggtg ctcggggccg gcgaacgccg
     1921 cgggccagcc agcgaggcac gggaaatgta tgaggtggta gggtagatcc tccccctgaa
     1981 gggggaggcg gccgtcaggc cggtggggga agcccgcaat gattcagcag cacttccccc
     2041 tccgtcgcct gcggcgacac cccccgctag ggggaggatt ttgagcctaa cctgcaaacc
     2101 ccgccaaaaa ccccggcacc gcgtccgccg gcatcggccg gccgaagtga tagccctgca
     2161 cctggtcgca gccatagccg atcagcttgt ccaactgggc ctgatcctcg acgccctcgg
     2221 ccaccacctt catgcccatg gacgagccca gggtgatcac cgccttgacg atggcctcgt
     2281 cctcggcgtt ctggacgaag gacttgtcga tcttcaggcg atcgatggga aactggcgca
     2341 gattggccag gctggcgtag ccggtgccaa aatcatccag ggcgatcatc acccccgcgt
     2401 cgtgcagccg cttgaccgtt tcgctgacca ggtccgagcc ccagcccata tagacattct
     2461 cggtgacctc gatcgtcagg cggtcgcacg gcacgcgcca gcgggccagg cgctcctgga
     2521 tctcctcggc cagccggccg ctgcggaact gggcggccga gatgttgacg gccacgcggc
     2581 caaactcgac gccggcgtcg atccaggtcc gcatctgctg cagggcggcc tcgaaggtca
     2641 catcgcccag cttcagcgac aggtcctgat cctcgaaggc ggccatgaac cgggccggcg
     2701 ccagaatgcc ctgctcggga tgggcccagc gcatcagggc ctcgaagccg gtgacgccgc
     2761 cgacccgcac atcgaccacc ggctggtaga acagcacgaa ctcgcccgcc gctatgccgg
     2821 cccgcacgtc gcgcagcagc tcgatccgct gctccagctc cgagcgcata gagggctgga
     2881 acatcacgct gcgattccgg cccgcgtcct tggcccgata gagggcgatg tcggcgttct
     2941 tgaccatgtg cgaggggtcg gcgtcaggat cgccgtgcag ggcggcgcca atgctggcgc
     3001 tgcaggtaaa gctgcggccg ccatgctcga tgggacgacg cagcagctcc tgcagggtct
     3061 ggatcggccg cagcatgtcg gcctcgccgt gcaggccgcg caggatcacc gcaaactcgt
     3121 cgccgcccag gcgggccacc gtgtcgtcgg cgcggaaggc gtggcgcagc atctcggcca
     3181 ggcgcttgag cagggcgtcg cccgcgtcgt gccccagcgt atcgttgatg tccttgaagt
     3241 gatcgacgtc gatcatgatc aggccgaaca tctcgcccag cagctccgac gcgctgaccg
     3301 cctcctgaaa gcgatgctgg aacagcgcgc ggttgggcag gtcggtcagg gtgtccttga
     3361 aggccagggt ctcgacagcc tcggtctggc gacggcggtc ggtgatgtcc ttgaacaggt
     3421 tgaccaggcg ctcggggcga ccgtgggccc cgaccagcac cttgctggcc gatcgcaccc
     3481 aggtctcgac cccatcgtcg cgattgagcc ggaactcgta gacccccgtc tcgcctggct
     3541 tgcgcgtcgc caccaaggcg gccacccggt cgcgatcgtc gcgatggatg atctccagcg
     3601 ggtcggtctt gaaccggcac cacttttcca gatccggcgt ggcgcccgaa aacaccagtt
     3661 cgcgggtgac caggtcatat tcgcggacca gcaggccatc cagctccagc gccatgcgca
     3721 gacgctgctg gtcgcgctcg gcgcgaagcg tggcctcggc atagcccgtg acgtcgcggg
     3781 cggtgaccat caccgcgtag ggcgtctcgc cctcgtcgcg gcaggcgcta aactcggtgc
     3841 gccagatgcg ctggacgccg tcggcgtccg gggccggcag atagcgcgag aaggcgtcgc
     3901 cggattcggc gcacttcatc agcgccagca cgtcttcggc ggcgaccagg gcgcgccgcg
     3961 catcgacgcc cgggcgcaga tcaggcgcga ggccatgggc ggtccactcg cggctcacgg
     4021 cgagcaggcg caggtcgcga tccagcagcg ccgcggcgaa cggcagctgg accacgaatt
     4081 gcgagaaagc gacggcgcgc gaatcgggca gacggctcgc tcctgtctcg gacgcgggcg
     4141 agcgcctgac ctccacggtc gacttgatca ttgtgtcggg ttctcg
//
LOCUS       DAOMEO010000126         4184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2560_length_4184_cov_2.234682, whole genome shotgun sequence.
ACCESSION   DAOMEO010000126 DAOMEO010000000
VERSION     DAOMEO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2560_length_4184_cov_2.234682"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..261)
                     /locus_tag="PLH31_03995"
     CDS             complement(43..261)
                     /locus_tag="PLH31_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88423.1"
                     /translation="MSETSTLVHDVSEEEIAEFTRGLNALGPQQRRILAVLIPRLIAL
                     EDRGDPEAAIRIIDEIRAILLTRRQACH"
     gene            complement(233..676)
                     /locus_tag="PLH31_04000"
     CDS             complement(233..676)
                     /locus_tag="PLH31_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88424.1"
                     /translation="MYQTFVPWISFSIAVLVCGFALWKGDYWARFVAAVYITGWVATP
                     FAQLRDPLSPEWGVMVIDVVVMIVLIWASLKARRLWSVFAAACQMMVVASHIVSIIDL
                     RIYIATVIMGLAMLAYGVLLALLVATFSAIRARRVAHERDLDPRS"
     gene            complement(743..1090)
                     /locus_tag="PLH31_04005"
     CDS             complement(743..1090)
                     /locus_tag="PLH31_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88425.1"
                     /translation="MKRIDAARAGAQKLMRAEAAIDAALRETAELVGLLPSLRLEANL
                     SAVIGQDAVEDLGQTLAHILSARRMIVRAHAGLQTVREQIGCRGVSVGDMDKPGDVPR
                     TGGLRTLNGGRAA"
     gene            complement(1305..1583)
                     /locus_tag="PLH31_04010"
     CDS             complement(1305..1583)
                     /locus_tag="PLH31_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88426.1"
                     /translation="MNDNQTRDTARIRQETSGFFIASWQSLEEGLTLLAGTPERGQLV
                     SETVSALRKSQTAGDPPAKLLLRVLAATAFLADDRPLRNEQGGAGAMT"
     gene            complement(1955..>3313)
                     /locus_tag="PLH31_04015"
     CDS             complement(1955..>3313)
                     /locus_tag="PLH31_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR88427.1"
                     /translation="AARMGREALKLEWNEDKAEKRSSSAIASSFRDIAAGKGPADLKW
                     EGFDSKGDAAVIANAKGAKVFEATYEFPYLAHATMEPMNCVAAVDGNKVKLTFGSQGQ
                     TLDQMNAAKIVGTLPGSVEIETLFAGGSFGRRATFQSDYAAECVHIAKKIGKGRPVKL
                     VWTREDDQRAGYFRPLVVHAVKISLDKDGYPAAWRHRIVSQSIMKGSPMGGKGPDQTA
                     IEGTAGSPYLKATPIVDAQLACPEVGVPVLWWRSVGATHTAFVMEHTIDQLARKAGKD
                     PIDYRRALYAKAGADRHLAALNLAIEKAGPAPTAGWTRGVAVHESFGSVVAQIAEVKL
                     VDGQPKVGRVVTAIDCGVAISPDQIAAQMEGGTCYGLSAALFGEITLTDGAVDQTNFD
                     SYRVLRMNEAPTVETYIVPSGSAPSGVGEPGTPVIAPAVANAILAATSKATARLPFTA
                     TV"
     assembly_gap    3314..3323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3324..>3692)
                     /locus_tag="PLH31_04020"
     CDS             complement(<3324..>3692)
                     /locus_tag="PLH31_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQR88428.1"
                     /translation="GEIAVKDSVVSHASGKTAGFGELLADAAKITPPANPALKDPKTF
                     TLIGTDRVRRKDSQAKSDGTARFTQDVRLPDMLTAMVAHAPRFGGKVKSFDAAEAKKV
                     PGVVDVFEIPTGVAVVAQNTY"
     assembly_gap    3693..3702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3703..4152)
                     /locus_tag="PLH31_04025"
     CDS             complement(<3703..4152)
                     /locus_tag="PLH31_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR88429.1"
                     /translation="MNGPLSKLIAQDGVSRRDIVVGATLVGGALLVGCSPAEIMSAGS
                     KVEVGAFGPFIKIAADGAVTVISKHVEFGQGNHAGLAAIVAEELDADWTRVRVEQAPA
                     NAKLYANGTAGVQLTGGSSAISNSWDQLRKAGAATRAMFVQAAAAKWS"
BASE COUNT          674 a         1414 c         1406 g          670 t
ORIGIN      
        1 gtgtgacctg gaaccttttc gcgacctccc gatgaactcg ctctagtgac aggcctggcg
       61 tcgggtcagc aggatggcgc ggatctcgtc gatgatgcgg atcgccgcct ccggatcgcc
      121 ccggtcctcc agcgcgatca gacgggggat cagcacagcc agaatccggc gctgctgcgg
      181 gccgagggcg ttcagaccgc gcgtgaactc ggcaatctct tcttccgaca cgtcatgaac
      241 gagggtcgag gtctcgctca tgagcgaccc gccgggcgcg aatcgccgaa aaggtggcga
      301 ccagcagggc cagcagcacg ccataggcca gcatggccag tcccatgatc acggtggcga
      361 tatagatgcg cagatcgatg atggaaacga tgtgcgaggc cacgaccatc atctggcagg
      421 ccgcagcgaa gacgctccac agccggcgag ccttcagcga ggcccagatc aggacgatca
      481 tgaccaccac atcgatgacc atcacgcccc attcggggct cagggggtcg cgaagctggg
      541 cgaacggcgt cgcgacccag ccggtgatat agaccgccgc gacgaaccgc gcccagtagt
      601 cgcccttcca cagggcgaac ccgcagacca gcaccgcgat gctgaacgag atccagggca
      661 caaaggtttg atacacggca tggtctcgac gcgaactggc cagagaccat gccgtaagct
      721 tcaccaagag agaaggccag gatcatgcgg cgcggccgcc gttgagcgtg cgcaagccgc
      781 ctgtacgcgg cacgtcgcca ggcttgtcca tatcgcccac cgacacgccc cgacagccaa
      841 tctgctcgcg caccgtctgc agcccggcat gggcgcggac aatcatgcgt ctggccgaga
      901 ggatgtgggc cagggtctgg cccaggtctt ccaccgcgtc ctgtccaatc accgccgaca
      961 ggttggcttc caggcgcagg cttggcagca ggccgaccag ctcggccgtc tcgcgcaggg
     1021 ccgcgtcgat cgccgcctcg gcgcgcatga gtttctgggc cccggcgcgg gccgcatcga
     1081 tccgtttcat gcaagactct ccggtcgcgc ggaggtcgtc cgcgcgttca agatctcgat
     1141 tttgggatag tcgccggccg ctcagcgcgg cggcggcggg ctccagccct cgcggaaccg
     1201 ctggagggcg tagaggaagt catgggctcc ggtggtcagg gcgccgagca gaagtccgcc
     1261 cacgatcgcc agccccacga tgagacccag ccattgcagg ggagtcaggt catcgcgcca
     1321 gctcccccct gctcgttccg cagtggccgg tcgtcggcca ggaaggccgt cgccgccagc
     1381 acgcgcagca acagcttggc gggcggatcg cccgccgtct gagacttcct cagggccgac
     1441 accgtctcgg aaaccagctg gccccgctcc ggcgtgcccg ccagcaaggt caggccctcc
     1501 tcgagggact gccagctggc gatgaagaag cccgaggtct cctgacggat ccgcgctgtg
     1561 tcccgggtct gattgtcgtt catggtcgtt cccacctgtc cggtgagaag agacccgcgt
     1621 cggggccaaa gctaggtcgc ccgattgcct acggtagtca ttgccggggt ccgccgcctc
     1681 ccaggccatg aaggccaggg ctgcatcgcg gcggctcagg cccgacaggc ggcggcgcgc
     1741 gctgagcacg tgcatctcga ccgtcctggg cgacaggctg agcaggcggg cgatttcctt
     1801 ggactgcaga tgactggcga tcaggcgcag aatctcccgc tcacgcgggg tcagacgctc
     1861 aaagccagga ctgtctccgg attccaccat gccgacagca tgcgaccagc gtctcgcgac
     1921 gtccagcgca agcgatgcgc cggacgtcag caggctagac tgtcgccgtg aaaggcaggc
     1981 gtgcggtggc cttgcttgtg gcggccagga tggcgttggc cacagcgggt gcgatgaccg
     2041 gtgtgcccgg ctcgcccacg ccgctgggcg cggaaccgga cggcacgatg taggtctcga
     2101 cggtcggcgc ctcgttcatc cgcaggacgc gatagctgtc gaaattggtc tgatccaccg
     2161 cgccgtcggt cagggtgatc tcgccaaaca gggccgccga gaggccatag caggtgccac
     2221 cctccatctg ggcggcgatc tgatcgggcg agatggcgac gccgcagtcg atggcggtga
     2281 ccacacggcc aaccttgggc tgaccgtcga ccagcttgac ctcggcgatc tgggcgacca
     2341 ccgagccaaa gctctcatgc accgccacgc cccgggtcca gccggccgtc ggcgcggggc
     2401 cggccttttc gatggccagg ttcagcgccg ccagatggcg gtcggcgcca gccttggcat
     2461 agagcgcgcg acgatagtcg atgggatcct tgccggcctt gcgggccagc tggtcgatgg
     2521 tgtgctccat gacgaatgcg gtgtgcgtgg cgcccaccga acgccaccaa aggacgggga
     2581 cgcccacctc agggcaggcc agctgggcgt cgacgatcgg ggtagccttc agatagggcg
     2641 agccggccgt gccttcgatg gcggtctggt cggggccttt gccgcccatc ggcgagccct
     2701 tcatgatcga ctggctgacg atgcggtgac gccaggcggc cggatagccg tccttgtcga
     2761 ggctgatctt cacggcgtgc accaccagcg gccggaaata gccggcccgc tggtcatcct
     2821 cgcgggtcca gaccagcttc accggccggc ccttgccgat cttcttggcg atatggacgc
     2881 attcggcggc gtagtcggac tggaaggtgg cccgccggcc aaacgagccg cccgcgaaca
     2941 gggtctcgat ctcgaccgag cccggcaggg ttccgacgat cttggcggcg ttcatctggt
     3001 ccagggtctg gccctgcgag ccgaaggtca gcttgacctt gttgccgtcc acggcggcca
     3061 cgcagttcat cggctccatg gtggcgtgcg ccagatacgg aaactcgtag gtcgcctcga
     3121 acaccttggc gcccttggcg ttggcgatca ccgccgcgtc gcccttggag tcaaagccct
     3181 cccacttcag gtccgccgga cccttgccgg ccgcgatgtc gcggaaggac gacgcgatgg
     3241 ccgacgagct acgcttctcg gccttgtcct cgttccactc cagcttcagc gcctcgcggc
     3301 ccatgcgggc ggcnnnnnnn nnngtaggtg ttctgggcga ccacggccac gccggtcggg
     3361 atttcgaaga cgtcgaccac gccggggacc ttcttggcct cggcggcgtc gaaacttttg
     3421 accttgccgc caaagcgcgg ggcatgggcg accatggcgg tcagcatgtc cggcaggcgg
     3481 acgtcctgag tgaagcgcgc cgtaccgtcg cttttggcct gcgagtcctt gcgacgcacc
     3541 cggtccgtgc cgatcagggt gaaggttttg gggtccttca gggccggatt ggccggcggg
     3601 gtgatcttgg cggcgtcggc cagcagctcg ccaaagcccg cggtcttgcc agacgcgtgg
     3661 ctcacgacgc tgtccttgac cgcgatctcg ccnnnnnnnn nngctccatt tggcggcggc
     3721 ggcctgaacg aacatcgccc gcgtcgcggc gccagccttg cgcagctggt cccaggaatt
     3781 ggaaatcgcc gaagagccgc ccgtcagctg cacccccgcg gtgccgttgg catagagctt
     3841 ggcgttggcc ggggcctgct cgaccctgac ccgggtccag tcggcgtcca gctcttcggc
     3901 gacgatggcg gcgaggccgg catggttgcc ctggccaaat tcgacatgct tggagatcac
     3961 cgtcaccgcg ccgtcggcgg cgatcttgat gaacggtccg aaggccccga cctcaacctt
     4021 ggaaccggcg ctcatgatct cggccggcga gcagccgacc agcagggccc ccccgaccag
     4081 ggtcgcgccc actacgatat cgcggcggct gacgccgtcc tgggcgatga gcttgctcag
     4141 gggcccgttc atcggccggc tccacggctg gtcgcgccgg tggc
//
LOCUS       DAOMEO010000127         4180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2562_length_4180_cov_2.342303, whole genome shotgun sequence.
ACCESSION   DAOMEO010000127 DAOMEO010000000
VERSION     DAOMEO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_4180_cov_2.342303"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLH31_04030"
     CDS             complement(<1..452)
                     /locus_tag="PLH31_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HQR88430.1"
                     /translation="MIDLSALDPETRRHAAAGAVALTWVAFCGLIAGRQALRKRAERR
                     EAAALAAGSGEPVLVAFASQTGFAEELALATAKALTAGGAPASLRDLSTVTAADLAGA
                     GRALFVVSTTGEGDAPDPARAFIRDVMAAEPDLSALRYGVLALGDTEY"
     gene            complement(449..1441)
                     /locus_tag="PLH31_04035"
     CDS             complement(449..1441)
                     /locus_tag="PLH31_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQR88431.1"
                     /translation="MTRVLVPLLTQPPARPLGGVVHALAGETMGTTWSVKLIAQPAAD
                     VPGLTAMVQHALDAVVAQMSPWEPLSALCRYNRAPAGSWMTLPAGMAAVVRRAIEIAE
                     ATDGAFDPTLGALTDLWGFGPRPFSGQPPSREAVEIAREVGGWTRLPLDGDALFQPGG
                     LRLDLNGIAKGFGVDQAAAALERAGVKSYLVEVGGELRGAGAKPDGQPWWVEMERAPA
                     ANDALQTIVALHDLSVATSGDYRRFFDHDGRRYAHTLDPASGAPTDQGMVSVTVLAKT
                     CMDADAWATALTVMGPEAALTFATAHALPALIVSRKGAGLEERLSPALSAMLDA"
     gene            complement(1604..2488)
                     /locus_tag="PLH31_04040"
     CDS             complement(1604..2488)
                     /locus_tag="PLH31_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HQR88432.1"
                     /translation="MSLKTRLLAVAAAGLALTLPMSANAHRGWMVPSFTVLSGDGSWV
                     TVDAAISNELFYPDHRAMRLDGVVVTAPDGSISKLQNASTGQYRSTFDVQLNQAGTWK
                     IGSASNSVTASWTENGEIKRFRGSAADFAKAVPAGATDLKTIKGSNRNETYVTRDKPS
                     TDVFKPTGKGLELVPVTHPTDIVATEAATFKFLLDGKPAAGLEVTFVPGGARWRATPG
                     EIKVKAGADGAVTFTLPDAGMYWVNATARGGESAGRGPGGGMGAPGAAPAAPAPVTAL
                     AGDGFSASYTAVLEAQRP"
     gene            complement(2501..3016)
                     /locus_tag="PLH31_04045"
     CDS             complement(2501..3016)
                     /locus_tag="PLH31_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HQR88433.1"
                     /translation="MKRSVSLLTFAGAAVGAAPAMAADLNVALEIPRLSVAEYHKPYV
                     AIWIEKPDATAAGTLAVWYDPDSKEDHGAKWLKDMRQWWRKAGREMKFPADGVSGATR
                     APGPHKVVFSGAKGALKTLKPGQYTLVVEAAREVGGHEAVRVPFTWGKPGKPASARGS
                     AELGAVTVSVK"
     gene            complement(3027..3656)
                     /locus_tag="PLH31_04050"
     CDS             complement(3027..3656)
                     /locus_tag="PLH31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HQR88434.1"
                     /translation="MSTATAQHRRSFWLKQLHQWHWISAALSLIGMLLFSITGITLNH
                     AGQIEAEPQVVSRKATLPADLIADLTKGPVEGKRPLPLKVQHWLDKAVGTDIAGREAE
                     WSDAEVYVALARPGGDAWLSIDRETGAVEHEKTTRGAVSLLNDLHKGRNTGKAWSWFI
                     DIFAAATIVFTVTGLILLQFHARARPLTWPLVAAGLAAPVILVILFVHL"
     gene            3916..>4180
                     /locus_tag="PLH31_04055"
     CDS             3916..>4180
                     /locus_tag="PLH31_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HQR88435.1"
                     /translation="MKALFFSASLLTLAATAAVAQQLPQPSAPTPAIAAAQDIPYPGT
                     LKLSVDATDLDRKIFQIRQTVPVAKAGKFTLLYPQWVPGGHSPR"
BASE COUNT          628 a         1374 c         1545 g          633 t
ORIGIN      
        1 ctgtattccg tgtcgccaag cgccagcacg ccatagcgca gggctgacag gtcgggctcg
       61 gcggccatca cgtcgcggat gaaggcgcgg gccgggtcgg gggcgtcgcc ctcgccggtg
      121 gtgctgacca cgaacagggc gcggccggcg ccggccagat cggcggcggt gacggtcgag
      181 aggtcgcgca ggctcgccgg cgcgccgccg gcggtcaggg ccttggcggt ggccagggcc
      241 agttcctcgg cgaaaccggt ctggctggcg aaggcgacca gcaccggctc gcccgagccc
      301 gcagccaggg cggcggcctc gcggcgctcg gcgcgcttgc gcagggcctg acggccggcg
      361 atcaggccgc agaaggcgac ccaggtcagg gccaccgcgc cggcggccgc gtggcgccgg
      421 gtctcgggat cgagggcgga aagatcgatc atgcgtccag catcgccgac agggcgggtg
      481 acaggcgctc ttccagaccc gcgcccttcc ggctgacgat cagggcgggc agggcgtggg
      541 cggtggcgaa ggtcaaagcc gcctcaggac ccatcaccgt cagggccgtg gcccaggcgt
      601 cggcgtccat gcaggtcttg gccagaaccg tgacgctgac catgccctgg tcggtgggcg
      661 cgccagaggc cgggtccaga gtgtgggcgt agcggcggcc gtcatggtcg aagaaccggc
      721 ggtagtcgcc gctggtcgcc accgacaggt catgcagggc cacgatggtt tgcagggcgt
      781 cattggcggc gggggcgcgc tccatctcca cccaccaggg ctggccgtcg ggcttggcgc
      841 ccgcgccgcg cagctcaccg cccacctcga cgaggtagct cttcaccccg gctcgctcca
      901 gggcggctgc ggcctggtcg acgccgaagc ctttggcgat gccgttgagg tccaggcgca
      961 ggccgccggg ctggaacagg gcgtcgccgt ccaggggcag acgcgtccag ccgccgacct
     1021 cgcgggcgat ttcgacggct tcgcgcgatg gcggctggcc ggagaagggg cgagggccga
     1081 agccccagag gtcggtcagg gcgcccaggg tggggtcgaa ggcgccatcg gtggcctcgg
     1141 cgatctcgat ggcccggcgc acgacggcag ccatgccggc gggcagggtc atccagcttc
     1201 cggcgggcgc gcgattgtag cggcaaagcg ccgacagcgg ctcccacggg ctcatctgtg
     1261 cgaccacggc gtcgagagcg tgctggacca tggcggtcag gcccgggaca tcggcggccg
     1321 gctgggcgat cagcttcacc gaccaggtcg tgcccatggt ttcgcccgcg agcgcatgga
     1381 ctacgccgcc cagcgggcgg gcgggaggct gggtcaggag ggggacgagg acgcgggtca
     1441 tgggggtttc aggctcgatc tcgatccttc cccctcgatg ggggaagggc agggatgggg
     1501 gtgaaaccgc cggccgattt cacgagggcc gcgccacatc ctgcggcgct gtcaccccca
     1561 tccccgaccc ttcccccatc aaagggggaa gggagcttct tgattaaggc cgctgggctt
     1621 cgagcacggc ggtgtagctg gccgagaagc cgtcgccggc cagggccgtc acgggggccg
     1681 gagcggcggg cgcggcgccg ggcgcgccca tgccgccacc cggaccgcga ccggcgctct
     1741 cgccgccgcg cgccgtggcg ttgacccagt acatgccggc gtccggcagg gtgaaggtca
     1801 ccgcgccgtc agcgccggcc ttgaccttga tctcgccggg cgtggcccgc cagcgcgcgc
     1861 cgccgggcac gaaggtgacc tcaaggccgg cggccggctt gccgtcgagc aggaacttga
     1921 aggtcgccgc ctcggtggcg acgatatcgg tcgggtgggt caccggaacc agttccaggc
     1981 ccttgccggt gggcttgaag acgtcggtgc tgggcttgtc gcgcgtgaca taggtctcgt
     2041 tgcgattgct gcccttgatg gtcttcagat cggtagcgcc ggccggaacg gccttggcga
     2101 aatccgcagc cgaaccccgg aagcgcttga tctcgccgtt ctcggtccag ctggcggtga
     2161 ccgaattgct ggccgagccg atcttccatg tgccggcctg gttcagctgc acgtcgaagg
     2221 tcgagcgata ctggccggtc gaggcgttct gcagcttcga gatcgagccg tcgggcgcgg
     2281 tgacgaccac gccgtccagg cgcatggcgc ggtggtcggg atagaacagc tcgttggaga
     2341 tggcggcgtc gaccgtcacc cacgagccgt cgcccgacag cacggtgaac gagggcacca
     2401 tccagccccg gtgggcgttg gccgacatcg gcaaggtcag ggcgaggccg gctgcggcga
     2461 cggcgaggag gcgggtcttc agggacatcg gttttctcgc ttacttgacg gagacagtga
     2521 cggcgccgag ttcggccgag ccccgggccg acgcgggctt gcccggcttg ccccaggtga
     2581 agggcacgcg gacggcttca tggccgccga cctcgcgggc cgcctcgacc accagggtgt
     2641 attggccagg cttgagggtt ttcagtgcgc ccttggcgcc gctgaacacc accttgtgcg
     2701 ggccgggggc gcgggtggcg ccgctaacgc catcggccgg gaacttcatc tcgcggccgg
     2761 ccttgcgcca ccactggcgc atgtccttca gccacttggc gccgtggtct tccttggagt
     2821 ccggatcata ccagaccgcc agggtgccgg cggcggtggc gtcgggcttt tcgatccaga
     2881 tggccacata gggcttgtga tactcggcca ccgacaggcg ggggatctcg agggcgacgt
     2941 tgagatcggc ggccatggcg ggggccgcgc cgacagcggc gccggcgaag gtcagcaggg
     3001 agacggaacg cttcacggat ttcacctcag aggtggacaa agagaatgac gagaatgacg
     3061 ggagcggcca ggccggcggc gaccaagggc caggtgagcg gccgggcgcg ggcatgaaac
     3121 tgcagcagga tcaggccggt gaccgtgaac acgatcgtgg cggcggcgaa gatgtcgatg
     3181 aaccacgacc aggccttgcc ggtgttgcgg cccttgtgca ggtcgttgag cagggacacc
     3241 gcgccgcgcg tggtcttctc gtgctcgacg gcgccggtct cgcggtcgat ggacagccag
     3301 gcgtcgccgc cgggacgggc cagggcgaca tagacctcgg cgtccgacca ttcggcctcg
     3361 cgcccggcga tgtcggtccc gacggccttg tccagccagt gctgcacctt caggggcaga
     3421 gggcgcttgc cctcgaccgg gcctttggtc agatccgcga tcaggtcagc cggtagggtg
     3481 gccttgcggc tgaccacctg gggctcggcc tcgatctgac cggcgtggtt caacgtgatg
     3541 cccgtgatcg agaacagcaa catgccgatc aggctcaggg ccgcgctgat ccagtgccac
     3601 tggtgcaact gcttgagcca gaacgagcgg cgatgctggg cggtggcggt cgacacgagg
     3661 gacgggaaac cttgagcggg aacaggtctc gtcatacttg agaacgactt gcaatttcaa
     3721 gagctgatcg tgtcaggatt tgtttccgcc gcgaccgccc cggggagcat atcgttcaac
     3781 gagatggccg taccaatcct ggtggccaga tcacgtcgtt cggtcaactt ggcctcgaca
     3841 accggcgatt aacacaagaa acattccgcc ttctgaccgc cgggagcatg ctggcgggcc
     3901 gtatcgaggt gtttcgtgaa agcgctcttc ttctccgcgt cgcttctgac tctggccgcc
     3961 acggctgccg tcgcccagca actgccgcag ccgtcggccc cgaccccggc catcgcggcg
     4021 gctcaggaca ttccctatcc gggcacgctg aagctgtcgg tcgacgccac cgatctggac
     4081 cgcaagatct tccagattcg ccagaccgtc cccgtcgcca aggctggcaa gttcacgctt
     4141 ctctacccgc agtgggttcc gggcgggcac tcgccacgca
//
LOCUS       DAOMEO010000128         4166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2571_length_4166_cov_2.897835, whole genome shotgun sequence.
ACCESSION   DAOMEO010000128 DAOMEO010000000
VERSION     DAOMEO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2571_length_4166_cov_2.897835"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1003
                     /locus_tag="PLH31_04060"
     CDS             38..1003
                     /locus_tag="PLH31_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HQR88436.1"
                     /translation="MQGLVTVFGGSGFVGSQVVRQLARAGHRVRVAVRQPHLAYKMRM
                     LGDVGQIEVVQANIRNVASVTRALEGAEACVNLVGLLHESGRQKFQAVHVMGARTIAE
                     RAKVAGVTRFVQISAIGADANSEAKYARTKAEGEAAVRAVFPGAVIVRPSVVFGPEDD
                     FFNRFAQMATISPALPLVGGETSFQPVFVGDVAAVVASAVSSPAAEGVTYELGGPTVY
                     TMRQILQLILTETGRDRALLPLPWPVAGLLGKVGDLMASVLPIAPPITSDQVEMLKSD
                     NVAEAGLPGLAEAGVTPTSVEAIVPAYLYRYRKGGQYAPTPEGAF"
     assembly_gap    1201..1210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1211..1920)
                     /locus_tag="PLH31_04065"
     CDS             complement(<1211..1920)
                     /locus_tag="PLH31_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR88437.1"
                     /translation="MRTLTQALLLTALLVLTQGCATVTPTGRVSVDGEAISHAVAGEG
                     RPAIVFEAGLGEGMDSWAEVFPAAATISKAFAYDRPGYGGSRTTRQQLGDVLDVLPGS
                     ELVALAALLEPDRPAINGAEAADRLDRVLTKSGLAPPYVLVGHSLGGQYVLIYAARYP
                     RKVAGVLLVDSRPKGFSTVCEARGLSPCKVPDLLRALSPEHIQAEIDGLVATDAKTPT
                     PGVLGDIPVIRLVATKPVP"
     gene            complement(1948..3825)
                     /locus_tag="PLH31_04070"
     CDS             complement(1948..3825)
                     /locus_tag="PLH31_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQR88438.1"
                     /translation="MAARSLPSRLAAGTAIGLLTLAGLVAATPARAQDLGRLFIFGDS
                     SADVGSDGARYTNRGEMWAETLARRLGRTSKPARAIPLDAIGGYDRAAPVSRLSGDNY
                     AVGGATAAAWTGTDIIGPSVSFADQVGFFRQDHPAGFARNDVVMVWMGGNDAANALLS
                     GVAHDSGRYAQAYMAQISALRQAGARDIIAFNEPAALLPVRYYQDILGLEPEDIEALM
                     EVHATRIAADNQALRPRLAQEGVYVLDLDRLTRDVLSRPDHYGFKATTDSYMTLGDPR
                     DLPDDGNVFADNHYSVAMQAVIADFALAQMEARNRFATLAAAPLERRRLADAALAPRL
                     EGPQAWMTPIGSVSLTASADVSRRDTRTRDLAPGQRRDVVGAGLGAHWRAGPSVVLGV
                     QADWARIEASAAGDRGGRQTRDLSATVYGLAQPLSGLTLSLALTGGQADHDEIERRTQ
                     LGGRAMAKARGETQARYGSVRLGAAYSLEAGDGWTLTPAVAVTGTRVRTDGYQEASGP
                     IALHYGKAMVNELRAGGAVTLADTHPDHAWRPLLTAGVEHVVDGDDVTVRVGPTAEQL
                     ARFRSERPARTWAHAAAGLDVALSPEARLGVSVGADRALGKGVDQKIQPHAQLALTLT
                     F"
BASE COUNT          652 a         1421 c         1439 g          644 t
ORIGIN      
        1 ctatcccccg ctgcatcgca ggcgacgagg aatgacaatg caaggtctgg tcacggtgtt
       61 cggcggctcc ggcttcgtcg gcagccaggt cgtgcggcag ctcgccaggg cgggccatcg
      121 cgtacgtgtc gcggttcgcc agccccatct ggcttacaag atgcgcatgc tgggcgatgt
      181 cggccagatc gaggtggttc aggccaatat ccgcaatgtc gcctcggtga cgcgggcgct
      241 tgagggggcc gaggcctgcg tcaatctggt ggggctgctc cacgaatcgg gccgccagaa
      301 attccaggct gtccacgtca tgggcgcccg gacgatcgcc gaacgcgcca aggtggccgg
      361 cgtcacccgc tttgtgcaga tcagcgccat cggcgccgac gcgaattccg aagccaaata
      421 tgcccgcacc aaggccgagg gcgaggcggc cgtgcgggcc gtgttccccg gcgcggtcat
      481 cgtccgcccc tcagtggtgt ttgggccaga agatgatttc ttcaaccggt tcgcccaaat
      541 ggccaccatc agcccggccc tgccgctggt tggcggcgag accagcttcc agcccgtgtt
      601 cgtcggtgat gtcgcggcgg tggtcgcgag cgcggtcagc agccccgcgg ccgagggcgt
      661 cacctatgag ctgggcggcc ccaccgtcta tacgatgcgt cagatcctgc agttgatcct
      721 gaccgagacc ggccgcgaca gggcccttct gcccctgccc tggcccgtgg ccggcctgct
      781 gggcaaggtc ggcgacctga tggccagcgt gctacccatc gccccgccga tcaccagcga
      841 ccaggtcgag atgctgaagt ccgacaatgt cgccgaggcc ggcctgccgg gcctggccga
      901 agcgggcgtg accccgacca gcgtcgaggc catcgtgccg gcctatctct atcgctaccg
      961 caagggcggc caatacgcgc ccacgcccga aggggcgttc tagagcgcga cagacggaac
     1021 ccgaccggcc tacggactga cctgggaggc cctatcgccc tggcgcgcca gatcgaccag
     1081 ttcgcgcagg gcctcggtca ccgcggcggg ccggtcgtcc atgatgtcgt ggctggcgcc
     1141 gtccaccagc tggacccgcc ccctggcctg ggccgcggcc tccgcgcgct gcatctcgac
     1201 nnnnnnnnnn ggaacgggct tggtggcgac cagccggatc acgggaatgt cgcccaagac
     1261 cccgggcgtc ggggtcttgg cgtcggtggc gaccaggccg tcgatctcgg cctggatgtg
     1321 ctcgggcgag agggcccgca gcaggtcggg gaccttgcag ggcgaaagtc cccgcgcctc
     1381 gcagacggtg ctgaaaccct tggggcgaga gtccaccagt agaaccccag ccaccttccg
     1441 aggatagcgg gcggcataga tcagcacgta ctggcccccc agggagtggc cgaccagcac
     1501 gtaaggcggg gccagtccgc ttttggtgag aacacgatca agccgatctg ccgcttctgc
     1561 gccgttgatc gcggggcgat ccggttccag cagggcggcg agcgccacca gttccgaccc
     1621 tggcagcacg tccagcacgt cgcccagctg ctgccgggtc gttcggctac cgccatagcc
     1681 cggccgatca taggcgaagg ctttgctgat cgtcgctgcg gcaggaaaga cctcggccca
     1741 gctgtccatg ccctcgccga gtcctgcctc aaagacgatg gcggggcggc cctcgccggc
     1801 cacggcatgg ctgatcgcct cgccgtccac gctgacccgg ccggtcggcg tcaccgtcgc
     1861 gcagccctgg gtcagaacga gcagcgcggt cagcaacagc gcctgggtca acgtcctcat
     1921 ggtggggtcc ttcctaggat cggcggatca gaacgtcagg gtcagagcca gttgggcgtg
     1981 gggctggatc ttctgatcga cgcccttgcc caaagcgcgg tcggcgccga cgctgacgcc
     2041 cagccgggct tccggcgaca gcgcgacgtc cagtccggct gcggcatggg cccaggttcg
     2101 ggctgggcgc tccgacctga agcgcgccaa ctgttccgcc gtcggtccga cccgcaccgt
     2161 cacgtcgtcg ccgtcaacca cgtgctccac cccggccgtc agaaggggtc gccaggcgtg
     2221 gtctggatgc gtgtcggcga gggtcaccgc gccgccggcc cgtagctcgt tcaccatggc
     2281 cttgccatag tgcagggcga tcgggccgct tgcctcctga tagccatcgg tgcgaacccg
     2341 ggtcccggtc actgccaccg cgggcgttag ggtccagcca tcgccggcct ccaggctata
     2401 ggcggcgccc aggcgcacgg agccatagcg ggcctgggtt tcaccccgcg ccttcgccat
     2461 cgcgcggccg cccagttgcg tgcgtcgctc gatctcgtcg tggtcagcct gaccgcccgt
     2521 cagggcgagg ctgagggtca gtccgctcag cggttgcgcg aggccgtaga ccgtcgccga
     2581 caggtcgcgc gtctgacgtc cgccccggtc acccgccgcc gaagcctcta tcctggccca
     2641 gtcggcctgc acgccgagaa ccacagacgg gcccgcccgc cagtgggccc ccagtccggc
     2701 gccgacgacg tcacgacgct gtcccggagc aagatcgcgc gtgcgcgtgt cgcgccgaga
     2761 aacatcggct gaggcggtca ggctgaccga accgatcggc gtcatccagg cctgcgggcc
     2821 ctcaagccgc ggcgccaggg cggcgtcagc caggcgtcgg cgctccaggg gtgcggccgc
     2881 cagcgtcgca aaccggttcc gggcttccat ctgggccaga gcgaaatcgg cgatcaccgc
     2941 ctgcatggcg accgagtagt gattgtccgc gaagacgttg ccgtcatccg gcaaatcacg
     3001 gggatcgccc agggtcatgt agctgtcggt tgtcgccttg aagccgtaat ggtcgggtcg
     3061 ggacagcacg tcccgggtca ggcgatccag atccaggaca tacacgccct cctgggccag
     3121 tcgcgggcgt agcgcctggt tgtcggcggc gatcctggtg gcatggacct ccatcaaggc
     3181 ctcaatatct tccggctcca gccccaggat atcctggtag tagcgcacgg gaagcagagc
     3241 ggccggctca ttgaaggcga tgatgtcacg cgcgcccgct tggcgcaggg cgctgatctg
     3301 ggccatgtag gcttgagcgt agcgtccgct gtcatgggcg acccccgaca gcagggcgtt
     3361 ggcggcgtca ttgccgccca tccacaccat caccacatcg tttctggcga agcctgcggg
     3421 gtgatcctgg cggaaaaagc cgacctggtc ggcgaagctc acgctgggcc cgatgatgtc
     3481 cgtgccagtc caggccgcgg cggtagcccc gccgacagca tagttgtcgc cagacaaccg
     3541 gctgacaggg gctgcgcgat catagccgcc gatggcgtcc agcgggatgg cgcgcgccgg
     3601 tttggaggtg cggcccagcc gacgggccag ggtctcggcc cacatctcgc cgcggttggt
     3661 gtagcgggcg ccgtcggagc cgacatcggc gctgctgtcg ccgaaaatga acagccgtcc
     3721 cagatcctgg gcccgagccg gcgttgcggc gaccaggcca gccagggtca gaagaccaat
     3781 ggcggttccg gccgccaggc gggaggggag agagcgcgcg gccatgatgg ggcctccaga
     3841 cgttgaaaac atggaaggca gctttgccgg gtcgcatgga ggcggcatgg agacccgatg
     3901 gacgcaggcg ggagatgttg gggttcggcg cggttcccgc atcttcacga gaccgccatt
     3961 gaccctccat ccgacctcca caaaggtgcg gtttcccaag ggcgggctgg accgggaggc
     4021 agggtagaca tgaacaacgc cctgatcctg atcgtggagg atgagcccca gatcgcggag
     4081 atcctggagg cgtatctggc ccgggatggc tttcgaacgg tcacggcccg ggatggtgaa
     4141 acggcgctgc agcatcatgc ccgcct
//
LOCUS       DAOMEO010000129         4159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2572_length_4159_cov_2.700536, whole genome shotgun sequence.
ACCESSION   DAOMEO010000129 DAOMEO010000000
VERSION     DAOMEO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2572_length_4159_cov_2.700536"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH31_04075"
     CDS             complement(<1..209)
                     /locus_tag="PLH31_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88439.1"
                     /translation="MSRVAIFVDAGYFLTQGSVTITGSKQARSELRLNGAAAQAALIQ
                     FAREKSDNARLLRTYWYDAQLRTGPT"
     gene            complement(366..1781)
                     /gene="murD"
                     /locus_tag="PLH31_04080"
     CDS             complement(366..1781)
                     /gene="murD"
                     /locus_tag="PLH31_04080"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQR88440.1"
                     /translation="MIPVRGFEGKTVAVFGLGRTGLTAARALVAGGANVALWDENPAS
                     QDAAMAEGFKVVDLRSADWSLFSALMLSPGVPLTHPQPHWTVKMARDAGVEILGDIEL
                     FARTVNAAPAHKRPKIIAITGTNGKSTTTALIGHLCAAAGRDTRVGGNIGAGVLGLDD
                     MHGGAVYVLELSSYQLDLTSSLKADATVLLNISPDHLDRHGGMEGYIAAKRRIFLNQG
                     KGDTAIIGVDDAWCQQICTEITAANRRTIWPISAGKAMGRGVYALQGVLYDATGERVT
                     EMADLLRARSLPGRHNWQNACAAYAAAIAVGIPAHDAVEGLMTFPGLAHRMETVGKIG
                     RVRFVNDSKATNADAARQAMSSYPKFYWIAGGVPKAGGISDLHDLFPRVEKAYLIGEA
                     AEDFAATLEGKAPVRMCGSIEVATAAAFSDAMDSGEDAIVLLSPACASFDQFADFEAR
                     GEAFRAAVEGLGKIGAKAARG"
     gene            complement(1793..2905)
                     /gene="mraY"
                     /locus_tag="PLH31_04085"
     CDS             complement(1793..2905)
                     /gene="mraY"
                     /locus_tag="PLH31_04085"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQR88441.1"
                     /translation="MLYFLYEWLARSQEHVPLLNLLKYLTFRTGMSMLTAYIVAVAMG
                     SRFIRWMKSKQGKGQPIRTDGIARHVTEKSGTPTMGGFMILAGLFVGALLWADLRNIH
                     VWVVLLITGSYGVLGFMDDYAKVTKQTTAGLSSGQKLVAQFVVAIAATVLLILFAPRA
                     EGVVGMETSLVFPFFKALVINLGWFYVAFAAVTIAGFSNAVNLTDGLDGLAIVPVMIA
                     AATFGLITYLVGNYKFADYLNLHFAPGVGELAVLCGAIIGGGMGFLWYNAPPAKIFMG
                     DTGSLALGGALGAIAVCSKHELVLGIVGGLFVVEALSVIIQVGYFKKTGKRVFLMAPI
                     HHHFEKLGWAESTVVIRFWIVAMMLSFVGLATLKLR"
     gene            complement(2905..>4159)
                     /gene="murF"
                     /locus_tag="PLH31_04090"
     CDS             complement(2905..>4159)
                     /gene="murF"
                     /locus_tag="PLH31_04090"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQR88442.1"
                     /translation="GARDGHEFVVQTNDKGAAGALVSKPVPVPAVLVEDTFRGLERLG
                     EAARLRAPQARRGAVTGSVGKTSVTRAVEAGLRLAGKAHASVKSYNNHIGVPLTLARM
                     PRDTERAVFEVGMNHADEITPLSRFIRPHAVAITTVGPVHLENFADGEEGVARAKAEI
                     FAGLEPQGVAVLNADNPWFDLLSEEAAKAGASIWSFGSHEGVTAQLTGFDVHAGGATV
                     SVNLRGEAISFPILQTGVHWGPNSLCVMLMLEALGVSREIALQALATFEPIEGRGAET
                     VVRIDGGAFTLVDESYNANPVSMQAALKTLGARKVEGRRIAVLTDMLELGEGSARFHA
                     ELAVPLDAAKVDSVFLAGVHMKSLWEALPPTRQGGYAEVTEKLTAALTRAIRPGDVVM
                     VKGSNGSRAGVLARALAALDLGGRG"
BASE COUNT          701 a         1402 c         1410 g          646 t
ORIGIN      
        1 gtgggccctg tacgaagctg cgcatcgtac caataggtcc gcagaaggcg ggcattgtcg
       61 ctcttctcgc gagcaaactg aatcagggcg gcctgcgcgg cggccccatt gagccgcaat
      121 tccgaccttg cttgcttgga gcccgtaatc gtgactgacc cttgggtcag aaagtagcca
      181 gcatcgacga agattgcgac acgcgacatc ggacggcccc aaacgtagaa agggcccccg
      241 caggggccct caggtaggtc acagatcgat gtaagtaggt tcgacctacg acggacagga
      301 aagtagcgat cctgtagcaa gagtcaagga tcttatgccc aaccgcctac gcatatgccc
      361 agcccctacc cccgcgccgc cttggccccg atcttcccca gcccctcgac cgccgcccga
      421 aacgcctcgc cgcgcgcttc aaagtccgcg aactggtcga acgacgcaca cgccggcgac
      481 agcaggacga tggcgtcctc gccactgtcc atggcgtccg agaacgccgc cgccgtagcc
      541 acctcgatac tcccgcacat ccgcaccggc gccttgccct ccagcgtcgc cgcgaagtcc
      601 tcggccgcct cgccaatcag ataggccttt tccacgcgcg ggaagaggtc gtgcaggtcg
      661 ctgatgccgc cagccttggg cacgccgccg gcgatccagt agaacttggg atagctgctc
      721 atcgcctggc gggcggcgtc ggcgttggtg gccttgctgt cgttgacgaa gcgcacgcgg
      781 ccgatcttgc cgacggtctc catccggtgg gccaggcccg ggaaggtcat caggccctcg
      841 accgcgtcat gggccgggat gcccacggcg atggcggcgg cataggcggc gcaggcgttc
      901 tgccagttgt ggcggcccgg caggcttctg gcccgaagga gatcggccat ttcagtgacg
      961 cgctcgccgg tggcgtcgta cagcacgccc tgcagggcat agacgccccg gcccatggcc
     1021 ttgccggcgc tgatcggcca gatggtgcgg cggttggcgg cggtgatctc ggtgcagatc
     1081 tgctggcacc acgcgtcatc gacgccgatg atggcggtgt cgcccttgcc ctggttgaga
     1141 aagatccggc gcttggcggc gatatagcct tccatgccgc cgtgacggtc caggtggtcg
     1201 ggcgagatgt tgagcagcac cgtggcgtcg gccttgaggc tgctggtcag gtccagctgg
     1261 taggacgaca gctccagcac gtagacggcg ccgccgtgca tgtcgtccag gcccagcacg
     1321 ccggcgccga tattgccgcc cacgcgggtg tcgcggccgg cggcggcgca caggtggccg
     1381 atcagggccg tggtggtgga tttgccgttg gtgccggtga tggcgatgat cttgggccgc
     1441 ttgtgggccg gggcggcgtt gaccgtgcgg gcgaagagct cgatgtcacc caggatctcg
     1501 acgccagcgt cgcgggccat cttcaccgtc cagtgcggct ggggatgggt caggggcacg
     1561 ccgggcgaca gcatcagggc cgagaacagg ctccagtcgg ccgatcgcag atcgaccacc
     1621 ttgaagccct cggccatggc ggcgtcctgg ctggcggggt tctcatccca cagggccaca
     1681 ttggccccgc cggccacgag ggcgcgcgcg gccgtcaggc cggtgcggcc caggccgaac
     1741 accgcgacgg tcttgccctc gaaaccacgg acggggatca tgctctggct ccctaccgca
     1801 gcttcagggt ggcgaggccg acgaaggaca gcatcatcgc cacgatccag aagcggatca
     1861 cgacggtgga ttcagcccag cccagctttt cgaaatggtg gtggatcggg gccatcagga
     1921 agacccgctt gccggtcttc ttgaagtagc cgacctggat gatgacgctg agcgcctcga
     1981 ccacgaacag accaccgacg atgcccaaaa cgagctcgtg cttggagcag acggcgatgg
     2041 cgcccagggc gccgcccagg gccagcgagc cggtgtcgcc catgaagatc ttggccggcg
     2101 gggcgttgta ccagaggaag cccatgcccc cgccgatgat cgcgccgcac agcaccgcca
     2161 gctcgccaac gccgggcgcg aagtgcaggt tcagatagtc ggcgaacttg tagttgccga
     2221 ccagataggt gatcaggccg aaggtcgcgg cggcgatcat caccggcacg atggccaggc
     2281 catccagccc gtcggtcagg ttcacggcgt tggagaagcc cgcgatggtg acggcggcga
     2341 aggccacata gaaccagccc agattgatca ccagggcctt gaagaacggg aagaccaggc
     2401 tggtctccat gcccacgacc ccttcggcgc ggggcgcaaa caggatcagc agcacggtgg
     2461 cggcgatggc gaccacgaac tgggcgacca gcttctggcc gctggagagc ccggccgtgg
     2521 tctgcttggt gaccttggca tagtcgtcca tgaagcccag cacgccgtag ctgccggtga
     2581 tcagcagcac cacccagacg tggatgttgc gcagatccgc ccacagcagg gcgccgacga
     2641 acaggccggc caggatcatg aagccgccca tggtgggcgt gccggatttc tcggtgacgt
     2701 ggcgagcgat gccgtcggtg cggatcggct ggcccttgcc ctgcttgctc ttcatccagc
     2761 ggatgaagcg cgagcccatg gcgacggcga cgatataggc cgtcagcatc gacatgccgg
     2821 tcctgaaggt caggtacttc agcaggttca gcagcggcac atgttcctgc gaacgcgcca
     2881 gccattcata cagaaaatac agcatcagcc ccgtccccca agatcgagcg ccgccagggc
     2941 cctggcgaga acgccagccc tggagccgtt cgaccccttg accatcacca cgtcgcccgg
     3001 ccggatcgcc ctcgtcagcg ccgccgttaa cttttcggta acttcggcgt agccgccctg
     3061 ccgagtcggc ggaagcgcct cccacagcga tttcatgtgg acgcctgcaa gaaagacact
     3121 gtccaccttg gcggcgtcca gcgggactgc aagctcggcg tgaaaccggg cgctgccctc
     3181 gcccagttcg agcatgtcgg tcagcacggc gatccgccgg ccttcaacct tgcgcgcgcc
     3241 gagggtcttc agcgccgcct gcatcgagac gggattggcg ttgtagctct cgtccaccag
     3301 ggtgaaggcg ccgccgtcga tccggaccac ggtctcggcg ccgcgcccct cgatgggctc
     3361 gaaagtcgcc agggcctgca gggcgatctc gcgcgagacg cccagcgcct ccagcatcag
     3421 catcacgcac aggctgttgg gaccccagtg gacgccggtc tgcaggatcg gaaagctgat
     3481 cgcctcgccg cgcagattaa ccgagaccgt cgcgccgccc gcgtggacgt caaagccggt
     3541 cagctgggcg gtgacgccct cgtgcgagcc gaagctccag atgctggcgc cggccttggc
     3601 ggcctcttcg ctcagcaggt cgaaccaggg attgtcggcg ttcagcacgg ccacgccctg
     3661 cggctccagg ccggcgaaga tctcggcctt ggcgcgggcg acgccctctt cgccgtcggc
     3721 gaagttttcc agatgcaccg ggcccacggt ggtgatcgcc acggcgtggg gccggatgaa
     3781 gcgcgacagc ggggtgatct cgtcggcgtg gttcatgccc acctcgaaca cggcgcgctc
     3841 ggtgtcgcgc ggcatccggg ccagggtcag gggcacgccg atatggttgt tgtacgactt
     3901 gaccgaggcg tgggccttgc cggccaggcg caggccggcc tcgacggcgc gggtgacgct
     3961 ggttttgccg accgagccgg tgaccgcgcc gcgacgggcc tgaggggcgc gcaggcgggc
     4021 cgcctcgccc aggcgctcaa ggccccggaa ggtgtcctcg accagcacgg ccggcacggg
     4081 cacgggcttg gagaccagag ccccggccgc gcccttgtcg ttggtctgga ccacgaactc
     4141 atggccgtcc cgcgcgccg
//
LOCUS       DAOMEO010000130         4146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2576_length_4146_cov_2.900513, whole genome shotgun sequence.
ACCESSION   DAOMEO010000130 DAOMEO010000000
VERSION     DAOMEO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_4146_cov_2.900513"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLH31_04095"
     CDS             complement(<1..1058)
                     /locus_tag="PLH31_04095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR88443.1"
                     /translation="MKIEVVPALDLHPADIDRWSSLQVGQARLDSPFLSPCWTQAVAR
                     ARGATGKKASDIKVAILRDDEGQAMGFLPARVRADVGMPVGAPMCDYQALVSAPGVAV
                     DPRRLLAALHVHRLDFCHMQADDETLSRHGRGRADSWIVEVPQGYAAYEAQRRTAGSG
                     VLKDIDKKRRKAEREVGPAVFTAMSTSRGDFDQLIAWKRAQLVATGQTDYFRTPWVQA
                     LVEDLFETRGETFGGGLYTLHLGGALAAVHLHLRGAHTLHGWLIAHSPAFERYSPGLM
                     LFQDILKSMDSGPYDRLDLGAGDYRFKRELSNARQTVVFGFLGSPSPAAFVRSAAYGV
                     RQMVEALPLGRMSELPGKA"
     gene            complement(1173..3323)
                     /locus_tag="PLH31_04100"
     CDS             complement(1173..3323)
                     /locus_tag="PLH31_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HQR88444.1"
                     /translation="MRNLVLALLASTSLMTASPIVANAAEPVPVPAEARTPLSELGKD
                     DPYLWMEEIEGQRAMDWAKDRNGKTLPVLQGDPRYADLEAQALAILNAKDRVPGVSFA
                     GDGSLRNFWQDATHVRGLWRKTALESYRTAEPVWETILDIDALSKAENANWVFKGASC
                     LPPEDTRCLITLSDGGKDAVTVREFDTTTRSFVAGGFELPEGKQNYSWLDKDTLLVGR
                     EWEPGQVTKSGYAYVLKTVKRGQPLSAAQEVYRGTETDVSVSPFVLRDADGVTQAVIA
                     YRGVTFFESEKHLLTDAGPVKLDLPLKSSIQAYVAGQMVVLLEQDWAEKGFRTGDLIS
                     FDLAALKAAPTTAQATLVLRPTAKQSVEQVTTTRTKLVVAMLDNVKGAAKVLTHGAKG
                     WTSQDLALPANSAIGLGSSAEKDERLFVTVTGYLAPSTFWLADAGSLKLEQVKASPDR
                     FDASTHLVEQFEATSTDGTKIPYFVVRPKDVKYDGSAPTLLYAYGGFQVSMTPGYSGV
                     MGKLWLERGGTYVVANIRGGGEFGPAWHAAGLKENRQKVYDDFFAVSQDLIDRKITSP
                     RRLGIMGGSNGGLLMGVALTQKPQLYNAIVVQVPLFDMIRYSQIGAGASWVGEYGDPA
                     IPSENAVIAKYDPYSNLKAGQPYPEVFIETSTKDDRVHPAHARKAAARLEELGYPVLY
                     YENIDGGHAGAANLAETARRQALEYVYLSRKLMD"
     gene            complement(3466..>4146)
                     /locus_tag="PLH31_04105"
     CDS             complement(3466..>4146)
                     /locus_tag="PLH31_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQR88445.1"
                     /translation="DLLIIAFFIVAPTMRDHGLTFYVLDYAVATFLALDMAARAIAHG
                     DLKAWLKKPIVWVDLFVLATLLFPAWLLNLGFLRVVRLWTLIHSEFFWRTVGQRYDDT
                     RIEEIAKAASSLLTFVFVITGFVYSSFVGRYEGVSGYVDALYFTVTSLTTTGYGDITL
                     PGVWGKLLSIFTMLGGVSLFIGLIQSILRPHKVTYQCQDCGLRRHDPDAVHCKACGRM
                     LNIPDDGV"
BASE COUNT          698 a         1377 c         1389 g          682 t
ORIGIN      
        1 gccttgcccg gcagctcgga catccgcccc agcggcaggg cctcgaccat ctggcggacg
       61 ccataggcgg cgctgcgcac gaaggcggcg ggcgagggcg agccgagaaa gccgaacacc
      121 accgtctggc gggcattgga cagctcgcgt ttgaagcgat agtcgccggc ccccaggtcg
      181 agccggtcat agggcccgct gtccatgctt ttcaggatgt cctggaacag catcaggccg
      241 ggcgaatagc gctcgaacgc cggactgtgg gcgatcagcc agccgtgcag ggtgtgggcg
      301 ccgcgcaggt gcagatgcac ggccgccagg gcgcccccca gatgcagggt gtagaggccg
      361 ccgccgaagg tctctccgcg ggtctcgaac aggtcttcga ccagggcctg gacccagggc
      421 gtcctgaaat agtcggtctg gccggtggcg accagctggg cgcgcttcca ggcgatcagt
      481 tgatcaaagt ccccgcgcga cgtcgacatg gcggtgaaga cggccgggcc cacctcgcgc
      541 tcggccttgc ggcgcttctt gtcgatatcc ttcagcaccc cggagccggc cgtccggcgc
      601 tgggcctcat aggcggcgta gccctggggg acctcgacga tccaggagtc ggcgcggccg
      661 cgcccatgcc gcgacagtgt ctcgtcgtcg gcctgcatgt ggcagaagtc gaggcgatgg
      721 acgtgcaggg cggccagcag cctgcgcgga tccaccgcca cgccgggggc cgagaccagg
      781 gcctgatagt cgcacatcgg cgccccgact ggcatgccca catcagcgcg cacccgggcg
      841 ggcaggaagc ccatggcctg gccctcgtca tccctgagga tcgccacctt gatgtcgctg
      901 gccttcttgc cggtcgcgcc ccgcgcccgc gccacggcct gggtccagca cggcgacagg
      961 aacggcgagt ccaggcgcgc ctgtcccacc tgcaggctcg accagcggtc gatgtcggcg
     1021 gggtgcagat ccagcgcggg aacgacctcg atcttcaagg tgacttggct ctctggcggg
     1081 ccgcgacggg ctctcgttcc cgtcatctag gcccagatag ctttccccat cgttgacgag
     1141 gcgacaacct gcggtaacgc cgcctggtca gatcaatcca tcagcttgcg cgacagatag
     1201 acatattcca gcgcctggcg ccgcgcggtt tcggccaggt tcgccgcccc ggcgtggccg
     1261 ccatcgatgt tctcgtaata gaggacggga tagcccagtt cctccaaccg cgccgccgcc
     1321 ttgcgggcgt gggccggatg gacgcggtcg tccttggtcg aggtttcgat gaacacctcg
     1381 ggatagggct ggccggcctt caggttggaa taggggtcat acttggcgat gacggcgttt
     1441 tccgacggta tggccgggtc gccatactcg cccacccacg aggcgccggc cccgatctgg
     1501 ctgtagcgga tcatgtcaaa cagcggcacc tgcacgacga tggcgttgta gagctgcggc
     1561 ttctgggtga gggccacgcc catcagcagg ccgccattgc tgccgcccat gatccccagc
     1621 cgccggggcg aggtgatctt gcggtcgatc aggtcctgtg agacggcgaa gaagtcatca
     1681 tagaccttct ggcggttctc cttcaggccc gccgcgtgcc aggccgggcc aaactcgccg
     1741 ccgccgcgga tattggcgac cacataggtc ccgccgcgct ccagccacag cttgcccatc
     1801 acccccgaat agcccggcgt catcgacacc tggaaaccgc cataggcgta gagcagggtg
     1861 ggggcgctgc cgtcgtattt cacgtccttg ggccgcacga cgaagtaggg gatcttcgtg
     1921 ccgtcagtgc tggtcgcctc gaactgctcg accaggtggg tggaggcgtc gaagcggtcc
     1981 ggcgaggcct tgacctgttc cagcttcagc gagccggcgt cggccagcca gaaggtcgag
     2041 ggcgccagat agccggtgac ggtgacgaac agccgctcgt ccttttcggc gctggagccc
     2101 aggccgatgg cgctgttggc gggcagggcc agatcctgcg aggtccagcc ctttgcgcca
     2161 tgggtcagca ccttggccgc gcccttgacg ttgtcgagca tcgccacgac cagcttggtg
     2221 cgggtggtgg tgacctgttc caccgactgt ttcgccgtgg ggcgcagcac cagcgtggcc
     2281 tgagcggtgg ttggcgccgc cttcagggcg gccagatcga agctgatcag gtcgccggtc
     2341 ctgaagccct tctcggccca gtcctgctcc agcagcacca ccatctgacc ggccacatag
     2401 gcctggatcg agcttttcag cggcaggtcc agctttacgg ggccggcgtc ggtcagcagg
     2461 tgcttttcgc tctcgaagaa ggtcacgccg cgataggcga tcaccgcctg ggtcacgcca
     2521 tcggcgtcgc gcagcacaaa cgggctgacc gacacgtcgg tctcggttcc gcgatagacc
     2581 tcctgggcgg cgctcagcgg ctggccgcgc ttcaccgtct tcaacacata ggcatagccc
     2641 gacttggtga cctgacccgg ctcccactcg cggccgacca gcagggtgtc cttgtccagc
     2701 cagctatagt tctgcttgcc ctcgggcagc tcgaaaccgc ccgccacgaa gctccgggtc
     2761 gtggtgtcga actcgcggac cgtcacggcg tccttgccgc catccgagag cgtgatcagg
     2821 cagcgcgtgt cttccggcgg caggcacgaa gcgcccttga acacccagtt ggcgttctcg
     2881 gctttcgaca gggcatcgat gtcgaggatc gtctcccaga ccggctctgc ggtgcggtag
     2941 ctttccaggg cggtcttgcg ccaaaggccg cgcacatggg tcgcgtcctg ccagaagttg
     3001 cgcaaggacc cgtcgccggc gaacgacacg ccgggcaccc ggtccttggc gttgaggatc
     3061 gccagggcct gggcttccag atcggcatag cggggatcgc cctgcagaac cggcagggtc
     3121 ttgccattcc ggtccttggc ccagtccatg gcccgctgac cctcgatctc ctccatccac
     3181 agatagggat cgtccttgcc cagttcgctc agcggtgtgc gcgcctcggc ggggaccggg
     3241 acgggttcag cggcattggc gacaatgggg gatgcggtca tgaggctcgt ggacgctaac
     3301 agggcaagga caagattacg cacttaggga tccatccgct ggcgtgacag ggcggtggag
     3361 cgcgacgctg gctgagccag gccgggcccg ccgaggggcg aagcgccacc gtgaccccgg
     3421 acggtaccgg aaaaatctac cggcgccaga ggatctcccg cgcattcaga ccccgtcgtc
     3481 gggaatgttc agcatccggc cgcacgcctt gcagtgcacc gcgtccggat catgccggcg
     3541 caggccgcag tcctggcact gataggtcac cttgtgcggc cgcaggatcg actggatcag
     3601 gccgatgaac agcgacaccc cgcccagcat ggtgaagatc gacagcagct tgccccacac
     3661 gccaggcagg gtgatgtcgc catagccggt ggtggtcagg ctggtgacgg tgaaatacag
     3721 cgcgtcgaca tagccgctga ccccctcata gcgccccacg aaactggaat agacaaatcc
     3781 ggtgatcacg aacacgaagg tcagcagcga tgaggccgcc ttggcgattt cctcgatccg
     3841 ggtgtcgtca tagcgctggc ctaccgtgcg ccagaagaac tcgctgtgga tgagggtcca
     3901 taggcgaacc acccgcagga agcccagatt cagcagccag gcggggaaca gcagggtggc
     3961 cagcacgaac aggtccaccc agacgatcgg cttctttagc caggccttca ggtcgccatg
     4021 ggcgatggcg cgggcggcca tgtccagcgc caggaacgtc gccaccgcat agtccagcac
     4081 atagaaggtc aggccgtgat cgcgcatcgt cggcgccacg atgaagaagg cgatgatgag
     4141 cagatc
//
LOCUS       DAOMEO010000131         4128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2588_length_4128_cov_2.393322, whole genome shotgun sequence.
ACCESSION   DAOMEO010000131 DAOMEO010000000
VERSION     DAOMEO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2588_length_4128_cov_2.393322"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..796
                     /locus_tag="PLH31_04110"
     CDS             23..796
                     /locus_tag="PLH31_04110"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQR88446.1"
                     /translation="MTRLIPLQGVENLRDYGDYAAGLGRLKKGVLYRAAHQAEATDDD
                     LDALAALNIVTLVDLRRPNERERSPSRRWTGFSAEVIDNELGATGPDPWHEFLKSSDL
                     SEGSIQAYMVEYYQRAPFKERHLDLFSRYFRALAQARGPVLIHCAAGKDRTGILAALT
                     HHVAGVSDDDVIDDYLLTNDPVRFERRGPKFMEIIHELTGRTPTEAAVRAAMGVEAQY
                     LAAALAAMKEQFGSLDGYLEQAVGLNADTREAVRAHILL"
     gene            875..2284
                     /locus_tag="PLH31_04115"
     CDS             875..2284
                     /locus_tag="PLH31_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88447.1"
                     /translation="MSDVTGALARGKPAGLSKSALSWILHQGARDPYVILITIYIFAP
                     YFSRVLIGDPVKGQAAMAEIATIYGLITAFTAPLLGASIERYGPRKPFLLAVLVLMTP
                     LLLALWFATPTGGLSVGVVGLILIVLGVANNWGDVLNNSLLARACERGQEPLLSGLGF
                     ALANFVSVVLLLFMLWGFVLPGEVSWPGIPAAPLFGMPTDAHEPSRLSAPLAALVMVL
                     GAIPFFLWTHDAPRTGLSMAASLRHGVRLLVDTFSNLKGHRDAATFLAARMLYCDGMT
                     ALLVFGGLFAAGLMGWGALEMLAYGISLSIFGVLGGFLAPWLDKTLGPRRALQLEITA
                     CLIVLIATLGMGKDRILYFWPYDLAAQAPVWDGPLFRTLPEVIYLGLGLLIAVFVTAQ
                     YASSRTLLVRLAPPDRIAAFFGLFALSGTATVWLGSLLVALATKIFQSQVAGFLPIVG
                     LLLAGVLGLFLVKGGGRED"
     gene            2353..2850
                     /locus_tag="PLH31_04120"
     CDS             2353..2850
                     /locus_tag="PLH31_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQR88448.1"
                     /translation="MVSPQVAAQPIADGRDDFDFFIGRWRVSHHRLVGRLTGSTDWQD
                     FDGTTQTRKILAGLGNMDENLLNLPAGAYQAVTLRLFDPARAVWTIHWIDARMATVDA
                     PMIGRFVDGVGEFFGDDTLDGRLIRVRFRWFVDDADHCRWEQAFSPDGGASWETNWTM
                     AFERT"
     gene            2889..4128
                     /locus_tag="PLH31_04125"
                     /pseudo
     CDS             2889..4128
                     /locus_tag="PLH31_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
BASE COUNT          558 a         1436 c         1418 g          716 t
ORIGIN      
        1 cctggcgcgc taacacccgg tcatgacccg tctgatcccg ctgcaaggcg tcgagaacct
       61 ccgcgactat ggcgactacg ccgctggtct tggccgactg aagaagggtg tgctgtaccg
      121 cgccgcccat caggccgagg ccaccgacga tgacctggac gccctggcgg ccctgaacat
      181 cgtcaccctg gtcgacctgc gccgccccaa cgagcgcgag cggtcgccgt cgcggcgctg
      241 gaccggtttc tcggccgagg tgatcgacaa cgaactgggc gcgaccgggc cggacccctg
      301 gcatgagttc ctgaaaagct cagacctctc cgaaggctcg atccaggcct acatggtcga
      361 gtactaccag cgggccccgt tcaaggaacg ccacctcgac ctgttctcgc gctacttcag
      421 ggcgctggcg caggcccggg gaccggtgct catccactgc gccgccggca aggaccgcac
      481 gggcatactg gccgccctga cccaccacgt ggccggcgtg tcggacgatg acgtgatcga
      541 cgactatctg ctgaccaacg accctgtccg tttcgagcga cgcggcccca agttcatgga
      601 aatcatccac gaactgaccg gccggacgcc cacagaagcg gccgtccgcg cggccatggg
      661 cgtcgaggcc cagtaccttg cggccgcctt ggcagcgatg aaggagcagt tcggctcgct
      721 ggacggctat ctggaacagg cggtcggcct caacgccgac acccgcgagg ccgtgcgggc
      781 gcatatcctg ctctaactcg cgggtgacaa acgcgcgttt aaccgccacg ctcccctgaa
      841 gcgaccgcat cagacggcgc caaaggggta gggcatgagt gacgtcacgg gcgcgttggc
      901 gcgcggcaaa ccggcaggtc tgtcgaagag tgcgctcagc tggatcctgc atcagggcgc
      961 ccgcgacccc tatgtgatcc tgatcaccat ctacatcttc gccccctatt tctcgcgggt
     1021 gctgatcggc gacccggtca agggccaggc ggccatggcc gagatcgcca ccatctatgg
     1081 cctgatcacc gcctttacgg cccccctgct gggggcctcg atcgagcgct atggtccgcg
     1141 caagccgttc ctgctggccg tgctggtcct gatgacgccc ctgctgctgg ccctgtggtt
     1201 cgccaccccg acgggcgggc tgtcggtggg cgtggtgggc ctgatcctga tcgtgctggg
     1261 cgtggccaac aactggggcg acgtgctcaa caattcgctg ctggccaggg cctgtgagcg
     1321 cgggcaggaa ccactgctgt ctgggctggg cttcgccctg gccaatttcg tgtcggtggt
     1381 gctgctgctc ttcatgctgt ggggctttgt cttgcccggc gaggtgtcct ggccaggcat
     1441 tcccgccgcg ccgctgtttg gcatgcccac cgatgcgcac gagcccagcc gtctgtcggc
     1501 gcccctggcg gccctggtca tggtgctggg cgcgatcccg ttcttcctgt ggacgcacga
     1561 tgcgccgcgc accggcctgt cgatggcggc cagcctgcgc catggcgtgc ggctgctggt
     1621 cgacaccttc agcaacctga agggccaccg ggacgccgcc acgttcctgg ccgcgcggat
     1681 gctctattgc gacggcatga ccgccctgct ggtgtttggc ggcctgttcg ccgccgggct
     1741 gatgggctgg ggcgcgctgg agatgctggc ctatggcatt tcgctgtcga tcttcggggt
     1801 gctgggcggg ttcctggcgc cgtggctgga caagaccctg ggcccgcgcc gggcgctgca
     1861 gctggagatc accgcctgcc tgatcgtgct gatcgccacc ctgggcatgg gcaaggacag
     1921 gatcctctat ttctggccct atgacctcgc cgcccaggcc cctgtctggg atggcccgct
     1981 gtttcgcacc ctgcccgagg tgatctatct gggcctgggc ctgctgatcg cggtgttcgt
     2041 cacggcgcaa tacgcctcca gccgcaccct gctggtgcgc ctggccccgc ccgaccgcat
     2101 cgccgccttc ttcggcctgt tcgccctgtc gggcacggcg acggtgtggc tgggttcgct
     2161 gctggtggcc ctggcgacca agatcttcca gagccaggtc gccggcttcc tgcccatcgt
     2221 cggcctgttg ctggccggcg tgctggggct tttcctggtc aagggcggcg gccgggagga
     2281 ctgaaggcga gactgctgac acatcctgac agtcgcctgg tccagaatac gccctgtcag
     2341 agggagccgt tcatggtgag tccgcaggta gcagcccagc ccatcgccga tggccgggac
     2401 gatttcgatt tcttcatcgg ccgctggcgg gtgtcgcacc atcgcctggt cgggcggctg
     2461 acagggtcga ccgactggca ggacttcgac ggaacgacgc agacgcgcaa gatcctggcg
     2521 ggtctgggca atatggacga gaacctgctg aacctgccgg ccggcgccta tcaggccgtc
     2581 accctgcgcc tgtttgatcc ggcccgcgcc gtctggacca tccactggat cgacgcccgc
     2641 atggccacgg tcgacgcccc gatgatcggc cggtttgtcg acggcgttgg cgagttcttc
     2701 ggcgacgata cgctggacgg gcgcctgatc cgggtgcggt ttcgctggtt cgtcgatgac
     2761 gccgaccact gccgctggga gcaggccttt tcgcccgacg gcggggccag ctgggagacc
     2821 aactggacga tggccttcga acggacctga gcacccctcc ccaggaggcg gcgatagtgt
     2881 atcaacccgc catgcgccgc gtcgctctcc tgtccgcccc ttggcgcgag ccggtctggg
     2941 cccttcagcc gtttcgggac gagccgcacg cctgcgccct gctgtcgggc gggggcgagc
     3001 ggtggtccta tctgctgcgc gcgccgcagg ccgtgctgac cctggacgcc gccgacccgc
     3061 gcgacccgtt tgaggccctg gccgcgctga tcggcccgcg cctgccggcc gatcccgagg
     3121 ggccgccgtt ccagggcggg gtggtgggtc tgggcgccta tgagctgggc gaccgggtcg
     3181 agcaactggg cctctcccgc accggctggc cagacctgac ctgcgcccgc tatgccgccc
     3241 tgctggcctt cgaccaccag gagcgccgcg ttgtcgcggt ggggcgcggc gatacggacg
     3301 gcgaggccca ggcgcgcgcc gaggcggccc tgacctggct ggacgccccc gtcgatgcgc
     3361 ggccgccgat cgaagggccg ctgtgtcccg tcctcaccgc caccgacggc gaagcctatg
     3421 aggtcgccgt ggccgatgtg gtggcgcgca tccatggcgg cgagatcttc caggccaata
     3481 tcgcccgcgc ctggaccggg cggctggcgg agggcgctga tccctacgat ctgttcaccc
     3541 gcctgcgcgg tcagagcccg gcgccgttct cggcctattg gcggctggag ggccgggcgc
     3601 tggtctccaa ctcgcccgag cggttcctga aggtgcgcga cggcgccatc gagaccaagc
     3661 cgatcaaggg cacgcggcga cgcggggcga cgccgaccga agacgccctg atggcctccg
     3721 agctgtcggc cagcgtcaag gaccgggccg agaatctgat gatcgtggat ctgatgcgca
     3781 acgacctggc ccgggtcagc ccgcccggca gcgtcaaggt tccggagctt ttcagggtcg
     3841 agaccttcgc caatgtgcat cacctggtct cgacggtgac ggcgcggctg gcgccgggcc
     3901 tcaccgtggc cgacttgctg cgggccagtt ttccgccggg ctcgatcacc ggcgcgccca
     3961 aggtgcaggc catgacgctg atcgcgctcc tggagccgcc gcgcggtccc tattgcggtt
     4021 cgctgttctg ggccgggttc gacggcagtt ttgactccag cgtgctgatc cgcacggtcg
     4081 gcctcgtgcg cgacgccgac ggctggacgc ttgaggcccg ggccgggg
//
LOCUS       DAOMEO010000132         4116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2598_length_4116_cov_2.292539, whole genome shotgun sequence.
ACCESSION   DAOMEO010000132 DAOMEO010000000
VERSION     DAOMEO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_4116_cov_2.292539"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1455
                     /locus_tag="PLH31_04130"
     CDS             169..1455
                     /locus_tag="PLH31_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HQR88449.1"
                     /translation="MLTAILGLAPIVIGLLAISAMFSAAETSLTAASRARMHQLEREG
                     DKPAKRVNRLLSDQETMIGAVLLGNNLVNILASALATTVLTAAIPGPWGVAVATATMT
                     VLVLVFAEVPPKTLAILKSDDVARFLSGPTLLVVRLFGPIIYAIQWIVRRTLRLFGVR
                     LDMAVDVLAAHEEIRGAVEYHHSEGLVESGDRRMLGGVLDLSDMDVSEIMVHRKSIVM
                     CDADLSPRELVAEALEAQHTRIPLYRDDPDNIIGVLHARDLLRALAVSEGGIDTLDIA
                     TILREPWFIPDTTNLKDQLNAFLKRKSHFAIVVDEYGALQGLVTLEDILEEIVGEIDD
                     EHDTSVEGVRTQIDGSVHVDGHVTVRDLNRAMDWDLPEGEAVTIAGLVIHEAQTIPDP
                     GQTFIFYQHRFQILRRQRNQVTALRISAKLDEGVNF"
     gene            1660..2028
                     /locus_tag="PLH31_04135"
     CDS             1660..2028
                     /locus_tag="PLH31_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88450.1"
                     /translation="MKSLSALKVLVVEDEALVSMLVEDMLSDLGCSVIGPAAEIEEAL
                     RLAGSADIDAALLDVNLGGRPIFPVADALKARGVPFAFASGYGEAGLSEGHRGALVLQ
                     KPFREADLRRVLEELAAQTA"
     gene            complement(2037..2453)
                     /gene="cdd"
                     /locus_tag="PLH31_04140"
     CDS             complement(2037..2453)
                     /gene="cdd"
                     /locus_tag="PLH31_04140"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQR88451.1"
                     /translation="MSADDITTIEQDAAAALPALLARSYARYSNFTVAAAVIDEQGRA
                     HLGVNVENAAYPSGTCAEQTAIGAMVTAGSRRIARVLIAAGSNAVVQPCGACRQRIAE
                     FASDDCEIVSVQDGVATQRAPFWSLLPESFGPRDLG"
     gene            complement(2564..2836)
                     /locus_tag="PLH31_04145"
     CDS             complement(2564..2836)
                     /locus_tag="PLH31_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HQR88452.1"
                     /translation="MGSVSDTIRSKLEAAFSPSRLDLKDDSDRHHGHAGHTGAGESHF
                     NLVIEAKAFAGKARVARQRMVHQVLSEELAGPVHALSIIATAPGES"
     gene            2895..3545
                     /locus_tag="PLH31_04150"
     CDS             2895..3545
                     /locus_tag="PLH31_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQR88453.1"
                     /translation="MAQEFEYRPKFFDIRVRPPKEGESAQPDVLGLKPGEKRCDHPDC
                     RLAGSAKAPKARNMPNEHYWFCQKHAAEYNKNWNFFAGMNEAQIRAEQETEKVTGGRP
                     TWSMKADSRSREAAAMAARDARHIADPFGLFRAQQRKAEADRAAADRHLGKLERGALA
                     DLDLDGAADAATIRAKYKELLKRCHPDVNGGDRSAEHKLQRVIKAYKTLQKAGLTT"
     gene            3642..4079
                     /locus_tag="PLH31_04155"
     CDS             3642..4079
                     /locus_tag="PLH31_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88454.1"
                     /translation="MTDPDSGLSLANAPTPVTPTPFGIPWSIWFWGGLLSLTGAAFAL
                     APFQTASLLRTDLHPLAEPWARMFGLMLIAYSLAYHVAGWTGARTYMRASVVLRLCSP
                     LAIGALVFTGNLPKPWMVVGLVDLAGAIWTAIELAWRPKARPV"
BASE COUNT          698 a         1398 c         1343 g          677 t
ORIGIN      
        1 gatcaaccga cgggtcggat cagccagcgc gtggaagcgc cgcgaaagat cgggttcatg
       61 cttagccatt aagctaacta ttacgccttc ggccgaccgg ggaaagtgaa aacttcgtcg
      121 attggctaag ttttccatcg gacctccctg tcgtagaaca ccgccatgat cctcaccgcc
      181 atcctcggcc ttgcgcccat cgtcatcggc ctgctggcga tttcggccat gttctcggcc
      241 gccgaaacct cgctgaccgc cgccagccgc gcgcgaatgc accagctgga gcgcgagggc
      301 gacaagccgg ccaagcgggt caacaggctg ctgtcggatc aggagacgat gatcggggcg
      361 gtgctactgg gcaacaatct ggtcaacatc ctggcctcgg ccctggcgac tacggtgctg
      421 accgccgcca tccccggccc gtggggcgtg gcggtcgcca cggcgaccat gaccgtgctg
      481 gtgctggtgt tcgccgaagt gccgcccaag accctggcaa tcctgaaatc cgatgacgtg
      541 gcgcggttcc tgtcgggtcc caccctgctg gtcgtacggc tgtttggccc gatcatctac
      601 gccatccagt ggatcgtccg gcgcaccctg cgattgttcg gcgtgcggct ggacatggcc
      661 gtcgacgtgc tggccgccca cgaggagatc cgcggggcgg tcgagtatca ccactcagaa
      721 ggtctggtcg aaagcggcga ccggcgcatg ctgggcgggg tgctggacct gtcggacatg
      781 gacgtctcgg agatcatggt ccaccgcaag tcgatcgtga tgtgcgacgc cgatctgtcg
      841 ccccgcgaac tggtggccga ggccctggaa gcccagcaca cgcgcattcc gctgtatcgc
      901 gacgatcccg acaacatcat cggcgtgctg catgcccgcg atctgctgcg cgccctggcc
      961 gtgtctgagg gcggcatcga caccctcgac atcgccacca tcctgcgtga gccctggttc
     1021 atccccgaca ccaccaatct gaaggatcag ctcaacgcct tcctgaagcg caagagccac
     1081 ttcgcgatcg tcgtggacga gtacggcgcc ctgcagggcc tggtgacgct ggaagacatc
     1141 ctggaagaga tcgtcggcga aatcgacgac gagcacgaca ccagcgtcga gggcgtgcgg
     1201 acccagatcg acggctcggt gcatgtggac ggacatgtca ccgtgcgcga cctgaaccgg
     1261 gccatggact gggatctgcc cgagggcgag gcggtgacca tcgccgggct ggtcatccat
     1321 gaggcccaga ccatccctga cccgggccag accttcatct tctatcagca ccgcttccag
     1381 atcctccgtc gtcagaggaa tcaggtcacc gccctgcgca tcagcgccaa gctggatgag
     1441 ggtgtgaatt tctgaccccc gggcgggaac ccgcgcgttg acggagcgtt gtacggcccg
     1501 ttcgggcggg gcgggggcga cttagttgca tcatttgacg ggtatgttgg cgaaaaaccg
     1561 gacgatcgcg caacaggtgc gagacggcgg cttaagctgt gatagcgtaa aagcgagcgt
     1621 ctgcgcgggc ctttccacgc gcgtcacgga gctggcgata tgaagtcctt gtccgccttg
     1681 aaggtcctgg tggtcgaaga cgaggctttg gtctccatgc tggtcgagga catgctcagc
     1741 gatctgggct gctcggtgat cggccccgcc gccgagatcg aagaagccct tcgcctggcc
     1801 ggctcggccg acatcgacgc ggctctgctc gatgtcaatc ttggcgggcg tccgatcttc
     1861 ccggtcgccg acgccctgaa agcgcgcggc gtgccgttcg ccttcgccag cggctatggc
     1921 gaggcgggcc tgtcagaagg ccatcgcggc gccctggttc tgcaaaagcc cttccgcgag
     1981 gccgacctgc gccgcgtgct cgaagagctg gccgcccaaa ccgcctaagg cgcggcctaa
     2041 cccaggtcgc gcggcccgaa actctcgggc agcaggctcc agaacggcgc gcgctgggtg
     2101 gcgacgccgt cctgaacgga cacgatctcg caatcgtcgg acgcaaattc ggcaatccgc
     2161 tggcggcacg cgccgcaggg ctgaacgacg gcattcgagc cagccgcgat cagcaccctg
     2221 gcgattcgcc gcgaacccgc cgtgaccatg gccccgatcg ccgtctgctc ggcgcacgtc
     2281 cccgacggat aggccgcgtt ctcgacattg acgcccagat gcgcgcggcc ctgctcgtcg
     2341 atgacggcgg cggcgaccgt gaagttcgaa tagcgggcat aggatcgcgc cagcagcgcg
     2401 ggcaaggcgg cggcggcgtc ttgttcgatc gtggtgatgt cgtcggcgct catgccatcc
     2461 ctcgttgctt cagggcccaa aaggccccgc cgacgagatt gcgccgggga gagaggcaat
     2521 cagtcgcggg gggtacgcgc aagccccatc gtggtgaaag gcttcagctt tcgccgggcg
     2581 ccgtggcgat gatcgaaagg gcgtgaaccg gccccgccag ttcctcggag agcacctggt
     2641 gcaccatccg ctggcgcgcc acccgggcct tgccggcaaa ggccttggcc tcgatcacaa
     2701 gattgaaatg gctctcgccc gcgcccgtgt ggccggcatg gccgtggtgg cggtcgctgt
     2761 cgtccttcag gtccagcctg gaaggtgaaa aagccgcctc aagcttgctt cgaatggtgt
     2821 ctgatacgct gcccatgccc ctctctcgca tgtcaatcct tgaacggaaa tagctgcggc
     2881 ttactctagg gggcatggcc caagagtttg aataccgccc gaagtttttc gacatccgcg
     2941 tccgcccccc caaggagggc gagagcgcgc agccggatgt gcttggcctg aaaccgggcg
     3001 agaagcgttg cgaccatccc gactgccgtc tggccggatc ggccaaggcg cccaaggccc
     3061 gcaacatgcc caacgagcac tactggttct gccagaagca tgcggccgaa tataacaaga
     3121 actggaactt cttcgccggc atgaacgaag cccagatccg cgccgagcag gagaccgaga
     3181 aggtcaccgg cggtcgcccg acctggagca tgaaggccga cagtcgctcg cgcgaggccg
     3241 ccgccatggc cgcccgcgac gcccgccaca tcgccgaccc gttcggcctg ttccgggccc
     3301 agcagcgcaa ggccgaggcc gatcgcgccg ccgccgaccg ccatctgggc aagctggaac
     3361 gcggcgccct ggccgacctc gacctggacg gcgccgccga cgccgccacg atccgcgcca
     3421 agtacaagga actgctcaag cgctgccacc ctgacgtcaa tggcggcgac cgcagcgccg
     3481 agcacaagct gcagcgggtg atcaaggcct acaagacact gcagaaggcc gggctgacca
     3541 cctagggcca acaggcagcc ggggcgcccc ttgcggcccg ccccggctgc cgccattctc
     3601 cgcccgcgct cgccgggacg ggcgcgaggg atagccgcat catgaccgac cccgattcgg
     3661 ggctcagcct cgcgaatgcg ccgaccccgg tgacgccgac gccattcggc ataccgtgga
     3721 gcatctggtt ctggggcggc ctgctgtccc tgaccggcgc cgccttcgcc ctggccccgt
     3781 ttcagaccgc cagcctgctg cggaccgatc tgcatcccct ggccgagccc tgggcgcgga
     3841 tgtttggcct gatgctgatc gcctattcgc tggcctatca cgtggccggc tggaccggcg
     3901 cgcgcaccta tatgcgggcc tcggtggtgc tgcggctctg ctcgccgctg gcgatcggcg
     3961 ccctggtgtt cacgggcaac ctgcccaagc cgtggatggt ggtgggcctg gtcgacctgg
     4021 ccggcgccat ctggaccgcc atcgaactgg cttggcggcc caaggccagg cccgtctaga
     4081 gccctttccg acctgattgt ttcatcaggg tcgggc
//
LOCUS       DAOMEO010000133         4115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2599_length_4115_cov_2.260837, whole genome shotgun sequence.
ACCESSION   DAOMEO010000133 DAOMEO010000000
VERSION     DAOMEO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_4115_cov_2.260837"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1236
                     /locus_tag="PLH31_04160"
     CDS             193..1236
                     /locus_tag="PLH31_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR88455.1"
                     /translation="MATIHDVAERADVSPKTVSRVLNDHESVSAKTRDRVRAAMQELG
                     YQPSAVARSLRSQTSSSVGVLMGDPSGGYHTKIHHALLLACVETGRHLTVDLFDGPIT
                     GWQDRIRAFVADGGIREMVLLPPECDFGPLKALMRELEVRCVLISPTSPDPNFPAIAM
                     DDRAAAREVVEHLFSLGHTRIGHISGHPDHAASTHRRNGFLEAYAARGLPRPAPELFA
                     DGEFTFKTGVEAAKALLDIDEPPTAIFAANDDMAAATCMEAQRRGLRIPDDLSVVGFD
                     DAPIAAVIWPTMTTIRQPFEQMALRALQTFSAWNANDAAGKAAAPFLAQHSLIVREST
                     GPARTGPPPKRKT"
     gene            1294..>3148
                     /locus_tag="PLH31_04165"
     CDS             1294..>3148
                     /locus_tag="PLH31_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HQR88456.1"
                     /translation="MNRQTFQALALALMIGASATALSVQAQTATAAAPVAQPWLNPAL
                     DPDTRADLVVKAMTQDEKLTLVFGYFGADMKPKYERIEGSLPGSAGYVKGIPRLGIPE
                     QWETDAGIGVATQGSAKEFRERTALPSGMATTATWNPQLAYKGGAMIGSEARNSGFNV
                     MLAGGVNLVRDPRNGRNFEYGGEDPLLAGTMVGAQIKGIQSNNLVSTIKHYALNNQET
                     GRFVLSANIDEPSARMSELLAFQFAMEQSDPHAVMCAYNRVNSVYACENDWLLNTVLK
                     KDWGFRGYVMSDWGAVHSGAKSVNAGLDQQSAGRAFDKQDFLGEVLKADLASGAVSGA
                     RLDDMARRITRSLFASGVVDNPVKIAPIDLKANGLVSQADAEEGMVLLKNDKGLLPLA
                     KTAKIIAVIGAHADVGVLSGGGSSQVYPAGGRAVQGLLPATWPGPVIYYPSSPLKALQ
                     ARFPNARITFADGKDPAAAAALAAGSDLAIVFADQWTTESEDVWTLNLPKNQDATIAA
                     VAKANAKTVVVLETGGPIVMPWLNEVGAVLQAWYPGTNGGEAIARVLTGEVDASGRLP
                     VTFPKDVTQLPRPKLDGDPKKPDASFDVDYTIDGAAVGYKWFDLKKHEPLFP"
     assembly_gap    3149..3158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3159..3553
                     /locus_tag="PLH31_04170"
     CDS             <3159..3553
                     /locus_tag="PLH31_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HQR88457.1"
                     /translation="YGLSYTQFAYSNLKASDNNGVLTVSFDVRNVGDRTGKDVPQVYI
                     APRAGGWEAPKRLGGFQKVELAPGAVASVTLTVDPRLLSTWDTQANGWSIAAGEYDVA
                     LGSSSRSLTATSTVSLKARSLPVSPLAR"
     assembly_gap    3728..3737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3913..>4115
                     /locus_tag="PLH31_04175"
     CDS             3913..>4115
                     /locus_tag="PLH31_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR88458.1"
                     /translation="MSIIRVGIVGVGKIARDQHIPAVAADPGLELVAVASRSHADVGV
                     PAFESLEEMLDQGPELDAIALCTP"
BASE COUNT          732 a         1414 c         1268 g          681 t
ORIGIN      
        1 aaacaccaaa aatccctgac ccattttgtc tgatttatgt ctcttaaaat gacccatttt
       61 gacctgaata tgtccgcatg gatgacaccg tggacaacgt gagttaggac ttgccacgcg
      121 cctcgccttg cggccgccac cttcaccgtg gtgaatcgcg agaacagggg cgtcagtcgg
      181 gttgggaact taatggcgac gattcacgat gtggccgagc gggcggatgt ctcgcccaag
      241 acggtctctc gcgttctcaa cgatcatgag agcgtcagcg ccaaaacgcg cgaccgggtc
      301 cgtgcggcca tgcaggaact gggctatcag cccagcgctg tggcccgcag cctgcgctcg
      361 cagacctcct cgtccgtggg ggtcctgatg ggggatccca gcggcggcta tcacaccaag
      421 atccaccacg ccctgctttt ggcctgcgtc gagaccggtc gacacctgac cgtcgatctc
      481 ttcgacggac cgataacagg ctggcaggac cgtatccgcg ccttcgttgc ggacggcggc
      541 atccgggaga tggtcctgct accgcccgag tgcgactttg gcccgctgaa agccctgatg
      601 cgtgagctgg aggtgcgctg tgttctgata tcgccgacca gccccgatcc caacttcccg
      661 gccatcgcca tggacgaccg cgccgccgcc cgcgaggtgg tcgagcacct gttctctctg
      721 ggccataccc gcatcggcca catttcggga catcccgatc acgccgccag cacccatcgg
      781 cgcaacggct tcctcgaggc ctatgcggcg cgcggtttgc cgcgtccggc cccggaactg
      841 tttgctgacg gcgagttcac cttcaagacc ggggtcgagg ccgccaaggc cctgctggac
      901 atcgacgagc cgcccaccgc gatcttcgcc gccaatgacg acatggccgc cgccacctgc
      961 atggaggccc agcgccgagg ccttcggatt ccggacgacc tctcggtggt cggcttcgat
     1021 gacgcgccga tcgccgcagt gatctggccg accatgacca ccattcgcca gcccttcgaa
     1081 cagatggcgc tgcgagcctt gcagaccttc agcgcctgga acgccaacga cgccgctggc
     1141 aaggccgccg cgccgttcct ggcccagcac agcctgatcg tccgcgagtc gaccggcccg
     1201 gcccgcaccg ggccgccgcc gaaacgaaag acctgatccc tttgcttccg agccgataac
     1261 ggccggcggt ccagacaaga atacggagga aacatgaacc ggcagacatt ccaggccctg
     1321 gccctggcgc tgatgatcgg cgcgtccgcg accgcgctga gcgtccaggc tcaaacggcc
     1381 accgccgccg ctccggttgc gcagccgtgg ctaaatcctg cgcttgatcc cgacacccgc
     1441 gccgatctgg tcgtcaaggc catgacccag gacgaaaagc tgaccctggt gtttggctat
     1501 ttcggcgctg acatgaagcc caagtacgag cgtatcgaag gctcgctccc cggttcggcc
     1561 ggctatgtga agggcattcc gcgtctgggc attcccgagc agtgggaaac cgacgccggc
     1621 atcggcgtgg caacccaggg cagcgccaag gaatttcgcg agcgcaccgc cctgccctcg
     1681 ggcatggcca ccaccgccac ctggaatccg caactggcct acaagggcgg cgcgatgatc
     1741 gggtcggagg cccgcaattc gggcttcaac gtgatgctgg ccggcggcgt gaatctggtc
     1801 cgcgacccgc gtaacggccg caatttcgag tacggcggcg aggacccgct gctggccggc
     1861 acgatggtgg gcgcccagat caagggcatc cagtccaaca acctcgtctc gaccatcaag
     1921 cactacgccc tgaacaacca ggagacgggc cgcttcgtcc tcagcgccaa tatcgacgag
     1981 ccgtcggccc ggatgtcgga actgctggcc ttccagttcg caatggaaca gtccgatccg
     2041 cacgcggtga tgtgcgccta caaccgggtc aacagcgtct atgcctgcga aaacgactgg
     2101 ctgttgaaca ccgtcctgaa gaaggactgg ggcttcaggg gctatgtcat gtccgactgg
     2161 ggcgcggtgc actccggcgc caagtcggtc aatgcgggcc tggaccagca gtccgcaggt
     2221 cgggctttcg acaagcagga cttcctgggc gaagtgttga aggctgatct cgccagcggc
     2281 gccgtcagcg gcgcgcggct ggacgatatg gcccgccgga tcacccgctc gctgttcgcc
     2341 agcggcgtgg tcgacaaccc ggtcaagatc gcgcccatcg acctgaaggc caacggcctg
     2401 gtcagccagg ccgacgccga ggagggcatg gtccttctga agaatgacaa gggcctgctg
     2461 cccctggcca agaccgcaaa gatcatcgcg gtgatcggcg cccatgccga tgtcggcgtg
     2521 ctgtcgggcg gcggctcctc gcaggtctat ccggccggcg gccgcgccgt gcaggggctg
     2581 ctgccggcca cctggcccgg cccggtgatc tactatccgt cctcgccgct gaaggcgctg
     2641 caggcccgct tcccgaacgc caggatcacc ttcgccgacg gcaaggaccc ggccgccgcc
     2701 gcagccctgg ccgccggcag cgacctggcc atcgtcttcg ccgatcagtg gaccaccgag
     2761 tcggaagacg tctggaccct gaacctgccc aagaaccagg acgccaccat cgccgccgtc
     2821 gccaaggcta acgcgaaaac agtcgtggtg ctggagacgg gcggccccat cgtcatgccc
     2881 tggctcaatg aggtcggcgc ggtgctgcag gcctggtacc ccggcacaaa cggcggcgag
     2941 gccatcgccc gcgtgctgac cggcgaggtc gacgcttcag gccgcctgcc cgtgaccttc
     3001 cccaaggacg tgacgcaact gccgcgtccc aaactggatg gcgatcccaa gaagcctgac
     3061 gcctcgttcg acgtggacta cacgatcgac ggcgcggccg tgggctacaa gtggttcgac
     3121 ctgaagaagc acgagccgct gttcccgtnn nnnnnnnngc tatggcctgt cctataccca
     3181 gttcgcctat tcgaacctga aggcgtcaga caacaatggc gttctgaccg tcagcttcga
     3241 cgtcaggaac gttggcgacc gcaccggcaa ggacgtgccc caagtctata tcgcgccgag
     3301 ggccggcggc tgggaagcgc ccaagcgcct gggcggcttc cagaaggtcg aactcgcgcc
     3361 cggcgccgtc gcttcggtga cgctcaccgt cgatccgcgc ctgctttcaa cctgggacac
     3421 ccaggccaac ggctggtcga tcgcggcggg cgagtatgac gtggctctgg gttcgtcctc
     3481 gcgatcgctg acggcgacct cgacggtcag cctcaaggcc cggtcccttc cggtctcgcc
     3541 gctagccagg tagcgccggc gcagggtttt ggttcagccc cgtgaagccc aagtagcgtc
     3601 gtccccggac gacgctacct tctttgcatg gggaaccagt agcctctgcg acatattatc
     3661 cacgccattg ccctgacggc cgacgcaccg caaaactcag tcatcgatag gtccccaccg
     3721 tcgatatnnn nnnnnnngct ggccggggat gcgttccttt ccccggccag ctcccctctc
     3781 ccccattcag attcgacgcc ggcagctgga tcgtgcgacg acgcgatact ggcgcttgcg
     3841 aggatcgacg aaatcgcttg gcacccccca tttgtacgat aatagatcga caaagagtca
     3901 gggagcggca aagtgagcat catcagggtc gggatcgtcg gcgtcggcaa gatcgccagg
     3961 gaccagcaca tccccgccgt cgcggctgat ccgggcctcg aactggtcgc cgtagccagc
     4021 cgcagccatg ctgatgtcgg cgtcccggcc tttgagtctc tggaggagat gctcgaccag
     4081 ggccccgaac tggacgccat cgccctgtgc actcc
//
LOCUS       DAOMEO010000134         4114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2600_length_4114_cov_2.369796, whole genome shotgun sequence.
ACCESSION   DAOMEO010000134 DAOMEO010000000
VERSION     DAOMEO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_4114_cov_2.369796"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLH31_04180"
     CDS             complement(<1..1003)
                     /locus_tag="PLH31_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQR88459.1"
                     /translation="MAQSFTGKKRIRKSFGRIPEAVQMPNLIEVQRSSYEQFLQREVR
                     PGTRKDEGIEAVFKSVFPIKDFNERAVLEYVSYEFEEPKYDVEECIQRDMTFAAPLKV
                     KLRLIVFETEEETGARSVKDIKEQDVYMGDIPLMTDKGTFIVNGTERVIVSQMHRSPG
                     VFFDHDKGKTHASGKLLFAARVIPYRGSWLDFEFDAKDVVYVRIDRRRKLPATTFLYA
                     LGMDGEEILTTFYDVVPFEKRTTAGAEGWATHYKPERWRGVKPEFALIDADTGEEVAP
                     AGTKITARQAKKLADAGLKTLLLAPEALTGRYLARDAVNFENGEIYAEAGDELDVVSI
                     "
     gene            1562..2326
                     /locus_tag="PLH31_04185"
     CDS             1562..2326
                     /locus_tag="PLH31_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR88460.1"
                     /translation="MTATTGCSGCRDGAGFDLPITMAFQPIVNAATGAVFAHEALVRG
                     AGGESAASVLAQVSDTNRYAFDQQCRVTAIDLAAQLNMADQGANLSINFLPNAVYEPR
                     ACIRLTLAAAMRSGFPINRIIFEFTEAERLDTDHILNILRSYRAMGFQTAIDDFGAGY
                     AGLGLLTKFQPDIVKLDMDLIRDIDTSTVKQSIVGGVLRILSDLGVTPLCEGVETVGE
                     YEVLRALGVELMQGYLLAALATPVLPGQTTDISAVA"
     gene            complement(2338..3027)
                     /locus_tag="PLH31_04190"
     CDS             complement(2338..3027)
                     /locus_tag="PLH31_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HQR88461.1"
                     /translation="MNILMVLTSHDRLGETGEKTGFWLEEFAAPYYALKDAGATLVLA
                     SPAGGQPPLDPKSDAPDAQSEDTVRFKADPEAQKALAATLKLSEIQAADFDAVFYPGG
                     HGPLWDLAEDAHSIRLIEALAAAGKPVAAVCHAPGVLRHVKGPNGDLLVKGKAVTGFA
                     DTEEAAVGLTDVVPFLVEAMLKTAGGVYSKGADWAPYVQTDGLLITGQNPASSRPAAE
                     ALLAALAKTPG"
     gene            complement(3183..3341)
                     /locus_tag="PLH31_04195"
     CDS             complement(3183..3341)
                     /locus_tag="PLH31_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88462.1"
                     /translation="MLKLAGILLVLMIVFGVLGFVVDVAGGLAKIAFFVCLIGAGVSL
                     AVKAIRKD"
     gene            complement(3488..3868)
                     /gene="rplL"
                     /locus_tag="PLH31_04200"
     CDS             complement(3488..3868)
                     /gene="rplL"
                     /locus_tag="PLH31_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQR88463.1"
                     /translation="MSKLEKLVEELSTLSVLEAADLSKMLEEKWGVSAAAPVAVAAAG
                     GPAAPVEAAEEQTEFTVVLTNGGDKKINVIKEVRGVRPDLGLKEAKDLVEGAPQNVVE
                     NVSKAQADEVAKKLTDAGATVQVK"
     gene            complement(3914..>4114)
                     /locus_tag="PLH31_04205"
     CDS             complement(3914..>4114)
                     /locus_tag="PLH31_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQR88464.1"
                     /translation="GGILGQTTVLDEAAVRALATLPSLDQIRGKLIGLIQAPATKIAG
                     VLQAPAGQLARVFNAYATKDAA"
BASE COUNT          720 a         1308 c         1341 g          745 t
ORIGIN      
        1 ggatcgagac cacgtccagc tcgtcgccgg cttcggcgta gatctcgccg ttctcgaagt
       61 tgacggcgtc gcgggccaga taacggccgg tcagggcctc cggcgccagc agcagggtct
      121 tgaggccggc gtcagccagc ttcttggcct gacgggcggt gatcttcgtg ccagccggag
      181 ccacttcttc gccggtgtcg gcgtcgatca gggcaaattc cggcttcacg ccgcgccagc
      241 gctcgggctt gtagtgggtg gcccagcctt cggcgccggc cgtggtgcgc ttctcgaagg
      301 gcaccacgtc atagaacgtg gtcaggattt cttcgccgtc catgccaagg gcatagagga
      361 aggtggtggc gggcagcttg cggcgacggt cgatgcggac atagaccacg tccttggcgt
      421 cgaactcgaa gtccagccac gagccgcgat acgggatcac gcgggcggcg aacagcagct
      481 tgcccgaggc gtgggtcttg cccttgtcgt ggtcgaagaa cacgccaggg gagcggtgca
      541 tctgcgagac gatgacccgc tcggtgccgt tgacgatgaa ggtgcccttg tcggtcatga
      601 gcgggatatc gcccatgtag acgtcctgct ccttgatgtc cttgacggac cgggcgccgg
      661 tttcttcttc ggtttcgaac acgatcaggc gcagcttgac cttcagcggc gccgcgaagg
      721 tcatgtcgcg ctggatgcac tcttcaacat cgtacttggg ctcttcgaat tcgtacgaga
      781 cgtattcgag caccgcgcgc tcgttgaaat ccttgatcgg gaagacggac ttgaagaccg
      841 cctcgatgcc ttcgtccttg cgcgtgcccg gacggacttc gcgctgcagg aactgttcgt
      901 aggaggagcg ctgaacctcg atcaggttcg gcatctgcac agcttcgggg atacggccga
      961 aagacttccg gatccgcttc ttgccggtga aggattgcgc catgtttttc ccttgggcgc
     1021 ggatcgccgc gccatgtgtt tcgaagggcg cgtgcggaaa gactccgccc gtgcgttcgt
     1081 ttgatcctgc gtccaccctc gccgcacccg cctgagacgg gagcgccgga cgctggaatt
     1141 cccctgacag gcgaccggga ccggtagacc tgacgggcgc cccttcgcgc ggtcggaggg
     1201 cggctgaccg cgcctcgggg cgtaatatgc ggacacgcgc gtgcgcgctc cgccgaaatg
     1261 tcttcgtgag ccggcggata taggccaagc agctgtggat gaaaaggggt tttgttaaga
     1321 aaccgccgtt ctggcgaggg gcgcgacacg aaatcggcga cggtcaattc agaccggcca
     1381 cccattgaga taacgcgtta ttggagtgag atttcgtcca ggaacaggct tgggatttcg
     1441 tcgccgatcc gacattgcgc cgcctcctcg cagtcgagac cccaagaagc gcgcaaatac
     1501 attgtttagc tgttcgcctt agtctcagtc cggatcaatg gcgcggacct gaggctctga
     1561 catgacagca acgacgggat gcagcggttg ccgcgacggc gcgggctttg atctgcccat
     1621 caccatggcc ttccagccca ttgtcaatgc ggccaccggc gcggtcttcg cccatgaagc
     1681 gctggtgcgc ggcgcgggcg gcgaaagcgc ggccagcgtc ctggcccagg tctccgacac
     1741 caatcgctac gccttcgatc agcagtgccg cgtcacggcc attgacctgg ccgcccagct
     1801 gaacatggcc gaccagggcg ccaacctgtc gatcaacttt ctgcccaacg ccgtctatga
     1861 gccccgcgcc tgcatccgcc tgaccctggc ggccgcgatg cgctcgggct tcccgatcaa
     1921 ccgcatcatc ttcgagttca ccgaggccga gcggctcgac accgaccata tcctgaacat
     1981 cctgcgcagc tatcgggcca tgggcttcca gacggcgatc gatgacttcg gcgcgggcta
     2041 tgcgggtctg ggcctgctga ccaagttcca gcccgacatc gtcaagctgg acatggacct
     2101 gatccgcgac atcgacacca gcaccgtcaa gcagtcgatc gtcggcggcg ttctgcggat
     2161 tctcagcgac ctgggcgtca caccgctgtg cgagggtgtc gagaccgtgg gcgaatatga
     2221 ggtgctgcgc gcgctcggcg tcgagctgat gcagggctat ctgctggccg ccctggccac
     2281 gcccgtcctc ccgggccaga cgacggacat cagcgccgtc gcctgacccg gcaaggttca
     2341 gcctggcgtc ttggccaaag ccgccagcag cgcctcggcc gccgggcgcg atgacgcggg
     2401 gttctgcccg gtgatcagca ggccgtcggt ctgcacatag ggcgcccagt ccgccccctt
     2461 ggaatagacg ccgcccgccg tcttgagcat ggcttcaaca aggaacggca ccacgtcggt
     2521 caggccaacg gcggcttcct cggtgtcagc aaatccggtg acggccttgc ctttcaccag
     2581 caggtcgccg tttggaccct tcacgtggcg aaggacgccc ggcgcgtggc agaccgccgc
     2641 cacgggtttg ccagccgccg ccagcgcctc gatcaggcgg atcgagtgcg cgtcctcggc
     2701 cagatcccat agcggaccgt gaccgccggg atagaagacg gcgtcgaaat cggcggcctg
     2761 gatctcggac agcttcagcg tggcggccag ggccttctgg gcttcgggat cggccttgaa
     2821 gcgaaccgtg tcctcgctct gggcgtcggg ggcgtcgctc ttcggatcca ggggcggctg
     2881 accgccggcg ggcgaggcca gcaccagggt cgcgccggcg tccttcaggg cataataggg
     2941 cgccgcgaac tcctccagcc agaaaccggt cttctcgcca gtttcgccga gacgatcgtg
     3001 ggaggtcaga accatgagga tgttcattgg aggggctcgc aggattgtcg ggaagggacg
     3061 acagatgtgg tgcgggccgg ccgctctcga aaggggcgcg cgaagaacat cgtccagagc
     3121 cgacagaaaa cgggccgaag ctttacccgc agcgccggcc tgtcgcgctg ccgcaggacg
     3181 gctcagtcct tgcggatcgc cttgacggcc agcgacacgc ccgccccgat cagacagacg
     3241 aagaacgcaa tcttggccag accgccggcc acgtcgacga cgaagccgag gacgccaaag
     3301 acgatcatca ggacgagcag gattccggcg agcttgagca tgtcgatgaa acgccgttga
     3361 agagggtcgg ttgcctgcga gcttaccacc aaaagaaaac ggccccggaa gtttccctcc
     3421 agggccgctc tttaggtcgt gatcctggcc gaagccggga caacatcatt cgccaaggcg
     3481 aatgatgtta cttcacctgg acggtcgcgc cggcgtcggt cagcttcttg gcgacttcgt
     3541 cggcctgggc cttggagacg ttttcgacga cgttctgcgg agcgccttcg accaggtcct
     3601 tggcttcctt caggccgagg tccggacgga cgccgcggac ttccttgatc acgttgatct
     3661 tcttgtcgcc accgttcgtc agaacgacgg tgaactcggt ctgctcttca gcagcttcaa
     3721 ccggagcagc cgggccgcca gcggcagcga cggcgaccgg agcagcggcc gaaacgcccc
     3781 acttttcttc cagcatcttg gacagatcag cggcttcgag gaccgacagg gtggacagtt
     3841 cttcaaccag cttttcgagc ttggacatgt tcagtttcct aaaacgaatg agggatgtac
     3901 gcggagatga ccgttaggcg gcgtctttgg tcgcataggc gttgaagacg cgagcaagtt
     3961 ggccagccgg ggcctgcagg acgccagcga tcttggtcgc cggagcctgg atgaggccga
     4021 taagcttgcc acggatctga tccagcgacg gcagcgtggc gagcgcccgc accgcagctt
     4081 cgtcgagaac cgtcgtctgg cccagaatgc cgcc
//
LOCUS       DAOMEO010000135         4106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2602_length_4106_cov_2.552209, whole genome shotgun sequence.
ACCESSION   DAOMEO010000135 DAOMEO010000000
VERSION     DAOMEO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_4106_cov_2.552209"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLH31_04210"
     CDS             <1..558
                     /locus_tag="PLH31_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88465.1"
                     /translation="DRMEAFSEDLPTPREAVNIHQVLDRIRALVANGVADGLSLRETY
                     DPSLPPVWGDEDHLIQIFLNLVKNAAEAAHMRGDGRGAISIHTAWRPGVRVRGADGKA
                     ASGAPIEIRIQDNGPGVPQTLRDHLFQPFVTTKPNGTGLGLALVTKLVTAHGGLIDFE
                     SEPGRTVFRVLLPVAPEPSVGDASV"
     gene            555..1976
                     /locus_tag="PLH31_04215"
     CDS             555..1976
                     /locus_tag="PLH31_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQR88466.1"
                     /translation="MNAASKKILIADDDSSVRLVLSQAFTRLGYQVRATGNATTLLKW
                     VMDGEGDLVVTDVMMPDENVFDVLPRIRKERPKLPIIVMSAQNTLLTAVNAADAGAFE
                     YVSKPFDLDDVTAAARRALSRPADAEASKAQARAMRDERLPLIGRSAPMQEVYRTIAR
                     LVGADLTVLILGESGTGKELVARALHELGRRRDGKFVVINLAAVPRERVETELFGRGD
                     GDLGRLIEADGGTLFLDEIGDMPLDAQTRLLRVIDGTEPVLNPKTGRRPNVRIIAATN
                     RDLRGLIQQGLFREDLFFRLNVAPVRLPPLRDRTEDIPDLARTFLLRANREGLPAKTI
                     DSSALDRLKAHSWPGNVRELENLIRRMCALYAEELITARIVDRELQDHTPAVRSEEGP
                     LTLSMLVERHLSGYFADQPDGVPAAGLYDRVLQEVERPLIQLTLSATRGNQVRAAEIL
                     GLNRNTLRKKIQDLGVEMTRGRR"
     gene            2034..>4106
                     /locus_tag="PLH31_04220"
     CDS             2034..>4106
                     /locus_tag="PLH31_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQR88467.1"
                     /translation="MASVTSALRSEGPPLRFSRAWWRELLRSRYVIGGAYALAVILTV
                     TGVALASSPPKSGPISPASTAILIVLGFNLALILGVATVVGLRLFEMIDARANDAGAR
                     LHLRFVGLFSLAAVAPAVIVALFFGVLVNRGVEGWFSQRVRTVVENSAKVARSYVSEQ
                     TSYISEHIRPMAANLNEAAPAMSQSPVVFSHFLAGETVTSGFSAAYVLDRDGRILARA
                     EMPGAPAFLAPPPSTFEGTDTGEAMAQRFVSSDLFRALYRLKAFPDAYLYVVRPIDPG
                     ILSHLIEAEDSLISYRDAERSRVRIQAIFALSYAETALLVLVAAVWVGIAAANSIAGP
                     VAGLVEAAGRVSAGDLNARVDTEVGPDEIRALSNAFNMMTTDLQIQQAALRTASLDAE
                     TRRQFIETVLSGVSAGVMSLDSQGRISALNRRAVTLLSLQEDAVGLDLIEAAPEFDIV
                     IETILEGRSDAEVEVDLVRDGETRRLRVRGAGQVAEGLVLTFDDITRLVAAQRNAAWK
                     DVARRIAHEIKNPLTPIQLSAERIKRKYRKEITSDLETFDRCTETIIRQVGDIGRMVD
                     EFSAFARMPAPRFEPANLTEMLRQGVFAQRFVDAEIEVALHEPGEDVLIKCDARMVGQ
                     ALTNILKNAGEAVGARRLVTEEPAGRITATLVANDEELCVVIEDNGVGLPVRDRDRLT
                     EPYVTTREK"
BASE COUNT          709 a         1298 c         1318 g          781 t
ORIGIN      
        1 gaccggatgg aggcgttctc tgaagatctc ccgacgccgc gcgaggcggt gaatattcac
       61 caggtgctgg accgcattcg cgctctggtc gccaacggcg tcgccgacgg tctgtcgctg
      121 cgcgagacct atgacccttc attaccgcct gtttggggtg acgaagacca tctgatccag
      181 atcttcctga acctggtgaa gaatgccgcc gaagccgccc atatgcgcgg cgacggacgc
      241 ggcgcgattt cgattcatac cgcctggcgt cccggggttc gggttcgcgg cgccgacggc
      301 aaggctgcca gtggcgcgcc gatcgaaatt cgtatccagg acaatggccc tggcgtgccg
      361 cagacccttc gcgatcattt gttccaaccg ttcgtgacaa ccaagccgaa cggcacgggc
      421 cttggcctgg cgctggtcac caaactggtc accgctcatg gcggcctgat cgatttcgag
      481 tccgagcccg gacgcaccgt gttccgcgta ctgctgcccg tggcccctga accctctgtc
      541 ggagacgcgt cagtatgaat gccgccagta agaagatcct gatcgctgac gacgactcgt
      601 cggtgcggct cgttctcagt caggccttca cacggctggg atatcaggtg cgcgccaccg
      661 gcaatgcgac cactctgctg aagtgggtca tggacggcga gggcgacctg gtcgtcacgg
      721 acgtgatgat gccggatgag aacgtctttg acgtgctgcc gcgcatccgc aaggaacggc
      781 ccaagctgcc catcatcgtg atgagcgccc agaacacctt gctgacagcg gtcaatgccg
      841 ccgacgctgg tgcattcgaa tatgtttcca agcccttcga tctggacgat gtcaccgctg
      901 ccgcccgccg tgccctgtcg cggccggccg acgccgaggc ttcgaaggct caggcccggg
      961 ccatgcgtga cgaacgcctg ccgctgatcg gtcgttcggc gccgatgcag gaagtttacc
     1021 gcaccatcgc tcgtctggtc ggcgcggacc tgacggttct gatcctcggc gagagtggca
     1081 ccggcaagga actggttgct cgggcgctgc atgagcttgg ccgccgtcgc gacggaaaat
     1141 ttgtagtcat caacctggcc gcggtgccgc gcgaacgcgt cgagaccgag cttttcggac
     1201 ggggggacgg agaccttggc cgcttgatcg aggccgatgg cggcacgctg tttctggacg
     1261 agatcggtga catgccgctg gatgcgcaaa cgcgtctgct gcgcgtgatc gacggcaccg
     1321 aaccggtgct gaacccgaaa acaggccgca ggccgaacgt gcggatcatc gcagccacga
     1381 accgcgacct tcgcggtctg atacagcagg gcctgttccg tgaagacctg tttttccgcc
     1441 tcaacgttgc gccagtgcgc ctgccgccgc tacgcgaccg caccgaggac atcccggacc
     1501 tggctcgcac ctttctgctg cgcgccaatc gagagggcct gccggccaag acgatcgatt
     1561 ccagcgctct ggaccgtctg aaggcccatt cctggccggg caatgttcgt gaactggaaa
     1621 acctgatccg gcggatgtgc gcgctctacg cagaggagct gatcaccgca cgtattgtcg
     1681 atcgcgagct ccaggaccac acaccagccg tgcggtcgga ggaggggcca ctgaccctgt
     1741 cgatgctggt ggagcgccac ttgtctggct atttcgccga ccagcccgac ggcgttccag
     1801 cagcgggtct ctatgatcga gtccttcaag aggttgaacg gcccctgatc cagctgacgc
     1861 tgtcagccac gcgaggcaat caggttcgcg ccgccgagat ccttggcctt aatcgcaaca
     1921 cgctgcggaa aaagattcag gacctgggcg tcgaaatgac tcgagggcga cgctgaagct
     1981 tatttggcac atagctgttg attttggggc acacccactt ctagactgac tccatggcgt
     2041 cagttacgtc cgcattgagg tccgaaggcc cgccgctccg gttcagccgg gcttggtggc
     2101 gggagcttct gcggtcgcgc tatgtgatcg gcggggccta cgcccttgct gtgatcctga
     2161 cggtcactgg cgtggcgctg gcgtcctcgc cccccaagag cggtccgatc agtccggcca
     2221 gcacggcaat cctgatcgtt cttggcttca atctggcgct gattctcgga gtcgcgacgg
     2281 tcgttgggct gcgactgttc gaaatgatcg acgcgcgggc caacgatgcg ggcgcgcgcc
     2341 tgcatcttcg ctttgtgggc ctgttctcgc tcgcagccgt ggcgccggcc gtgattgtgg
     2401 ctcttttctt cggcgtgttg gtcaatcgcg gcgtcgaggg ctggttcagc cagcgggtcc
     2461 gaactgtggt cgagaactcg gccaaggtcg cgcggtccta tgtgtccgaa cagaccagct
     2521 acatcagcga acacatccga cctatggccg ccaatctgaa cgaggccgcg ccagcgatgt
     2581 cgcagtctcc cgtggtgttc agccacttct tggcgggaga gaccgtaacg agcggcttct
     2641 cggccgcgta tgtgctcgac cgggatggac gaattctggc cagggccgag atgcccggcg
     2701 cgcctgcgtt tcttgcgccg ccgccctcca cattcgaggg gaccgatacg ggcgaagcga
     2761 tggcgcagcg gttcgtgtcc tcggatctgt tccgagccct ttatcggctc aaggcctttc
     2821 cggacgccta tctctacgtt gttcgtccga tcgaccccgg gatcctgagc catctgatcg
     2881 aagctgagga ctcgctgatc tcctatcgcg acgccgagcg gagccgcgtt cggatccagg
     2941 cgatcttcgc actcagctat gccgaaaccg cgctcctggt gctggtcgct gcggtatggg
     3001 tcggcatagc cgcagccaac tctattgcag gccccgtggc gggcctggtc gaggccgctg
     3061 gccgcgtttc ggcgggcgat ctgaacgccc gcgtcgatac cgaggtggga cctgatgaga
     3121 taagggcatt atccaatgcc ttcaatatga tgactacaga tcttcagatc caacaagcgg
     3181 cgctccggac ggctagtctg gacgccgaga cccggcgaca gtttatcgaa accgtgcttt
     3241 ccggcgtcag cgccggggtc atgagtctgg actcccaggg acgtatttcg gcgctgaacc
     3301 gtcgcgccgt caccctgctg agccttcaag aggacgccgt tggactggac ctgatcgagg
     3361 ctgctccaga gttcgatatc gtcattgaga ccatcctgga gggccggtca gacgccgagg
     3421 tcgaggttga tctggtgcgc gacggcgaga cacggcgcct gcgggtgcgc ggcgccggcc
     3481 aggtagccga gggactggtc ctgacctttg acgatattac ccgccttgtt gcggcccagc
     3541 gcaacgccgc ctggaaagac gtcgcccggc ggatcgcgca cgagatcaag aacccgctga
     3601 cccccattca gctgtcggcg gaacgtatca agcgcaagta tcgcaaggag atcaccagcg
     3661 atctggagac cttcgatcgc tgcaccgaga ccatcatccg tcaggtgggc gatatcgggc
     3721 ggatggttga cgagttctcg gcgtttgcgc gcatgcctgc gccgcgcttc gagccggcca
     3781 acctcaccga gatgcttcgg cagggggttt tcgcgcagcg ctttgtcgac gccgagatcg
     3841 aggtcgcgtt gcatgagccc ggcgaggacg ttctgatcaa atgcgacgct cggatggtgg
     3901 ggcaggccct gaccaacatc ctcaagaacg caggcgaggc ggttggcgcg cgtcggctgg
     3961 tcaccgaaga gccggccggc cgcatcaccg cgaccttggt ggcgaacgac gaggagcttt
     4021 gcgtggtcat cgaggacaac ggcgtcggac tgcccgtccg cgaccgcgac cgcctcaccg
     4081 agccctatgt cacaacccgc gagaag
//
LOCUS       DAOMEO010000136         4092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2608_length_4092_cov_2.498638, whole genome shotgun sequence.
ACCESSION   DAOMEO010000136 DAOMEO010000000
VERSION     DAOMEO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_4092_cov_2.498638"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLH31_04225"
     CDS             complement(<1..526)
                     /locus_tag="PLH31_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HQR88468.1"
                     /translation="MRTDDLIDALAAQAGPDPGGSPSRRLALLVGLGVAVALAGVLIW
                     LKVRPDLMDVMSGGFFWLKAAYTGALGLAGYLAVERLARPLGSPRRGLVLGLTVLVGF
                     VLAGLVQFGLSDDPMTLLRGGSWTRCSLNILALGAPTTLLGLGVMRGLAPTRPTLAGF
                     AVGALAGGVAATVYG"
     gene            complement(513..1052)
                     /locus_tag="PLH31_04230"
     CDS             complement(513..1052)
                     /locus_tag="PLH31_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR88469.1"
                     /translation="MTDTETRLKALMLRGLEGDARAYRECLAVLGVRLRAYFARRMSG
                     APGDVEDMVQETLLAVHIKRATWDSSQSFTAWAHAVARYKLIDHWRRRKIRQTLPIDD
                     HAEFLAAEEHDPGVGLELDRALASLPDRQRILVSDVKLAGLSLAEAGARAGISEGAAK
                     VALHRALKALAERMRRADR"
     gene            1239..1517
                     /locus_tag="PLH31_04235"
     CDS             1239..1517
                     /locus_tag="PLH31_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HQR88470.1"
                     /translation="MNRNTATAALAAVFALSAGAALADDMKAKKPAMEKCYGVSMAGK
                     NDCAAGAGTSCAGTSKVDYQGNAWTYVAKGTCTTIKTPKGMGSLTPKM"
     gene            1631..2461
                     /locus_tag="PLH31_04240"
                     /pseudo
     CDS             1631..2461
                     /locus_tag="PLH31_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
     gene            2454..3035
                     /locus_tag="PLH31_04245"
     CDS             2454..3035
                     /locus_tag="PLH31_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQR88471.1"
                     /translation="MSDLLEFQDAFVEALAGGPTVLSPWLAAADAGEPGLSVYRNTIA
                     RGCVDALAANFPTVATLVGEDWFPPPPPCSPARPRPPTRPCWTMARTSPPGWTASRRP
                     RTCPIWRASPIWTAFGPRPCSPARRRPCPPKPSANWRPRPWPSPGCGCIRPSASPPSG
                     RACPACGPALARAATTSNSSKPPRACCSAGPTR"
     gene            3035..3220
                     /locus_tag="PLH31_04250"
     CDS             3035..3220
                     /locus_tag="PLH31_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88472.1"
                     /translation="MAYRIVGPADCAFLAACRMGRPLAEAMGLAAEADPGADLQILFA
                     ALIDAKIFSGLDTGPRK"
     gene            3217..3660
                     /locus_tag="PLH31_04255"
     CDS             3217..3660
                     /locus_tag="PLH31_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQR88473.1"
                     /translation="MTTALLKPFDRLAELAGKALPDDVLTLVARLGVAGIFWLSGRTK
                     VDGVLTVSDGAYALFSDEYALPLIPSDIAAHMATYAEHLLSILLVLGLLTRLSALGFL
                     GMTLIIQVFVYPDAWPTHLSWAGLLLLLVAKGGGRFSLDRALGIK"
     gene            complement(3682..>4092)
                     /locus_tag="PLH31_04260"
     CDS             complement(3682..>4092)
                     /locus_tag="PLH31_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HQR88474.1"
                     /translation="LRLYCTPERGFHEGRLGHDYAGVCPERAARDFLPAYADGKLVHA
                     ATTRLSEAKSDRSSADRRAEKRDREARGVEDELRNPALNDEQKRELRDRLNRLRSERR
                     AAVEEGRRADWALRDAEREVDDLEHRFRPIYGGW"
BASE COUNT          619 a         1576 c         1285 g          612 t
ORIGIN      
        1 ggccatagac cgtcgctgcg accccgccgg ccagggcccc gacggcgaac cccgccaggg
       61 tcggccgggt gggcgccaag ccccgcatca cccccaggcc cagcagcgtg gtcggcgccc
      121 ccagggccag gatgttcagc gagcaccggg tccacgagcc gccgcgcagc agcgtcatcg
      181 ggtcatccga aaggccaaat tgcaccagcc ccgccaggac aaaaccgacc agcacggtca
      241 ggcccagaac caggccgcgg cggggcgagc ccagcgggcg cgccaggcgc tcgaccgcca
      301 gatagcccgc cagccccagg gcgccggtat aggccgcctt caaccagaag aagccgccgg
      361 acatcacgtc catcaggtcc ggccgcacct tcagccagat cagcacgccg gccagagcca
      421 ccgccacgcc caggccaacc agcagggcca ggcgccgaga gggcgagccg ccggggtcgg
      481 gaccggcctg agcggccagg gcgtcgatca ggtcatcggt ccgcacggcg catcctttcg
      541 gccagggcct tcagcgcccg atgcagggcg accttggccg cgccctcgga aatgccggcc
      601 cgcgcgccgg cctcggccag gctcagaccc gccagcttga cgtcgctgac caggattcgt
      661 tgccgatcgg gcaggctggc cagggcgcga tccagctcaa ggccgacgcc ggggtcgtgt
      721 tcctcggcgg ccaggaactc ggcatggtcg tcaatgggca gggtctggcg gatcttgcgg
      781 cgacgccagt ggtcgatcag cttgtagcgc gccacggcgt gcgcccaggc ggtgaacgac
      841 tgggagctgt cccaggtcgc ccgcttgata tgcaccgcca gcagcgtctc ctgcaccata
      901 tcctccacat caccgggcgc gccggacatc cggcgtgcga aataggcgcg caagcgaact
      961 cccagcacag ccagacactc gcgataggcc cgggcatcgc cctccagccc gcgcagcatc
     1021 agcgccttca gtcgggtctc ggtgtccgtc acgctatccc ctattcgcgc gcctggccca
     1081 gatggttaca cggcgcgcgc ccgggccgaa accggccaga cgagggcccg gaaatttttg
     1141 ttcggcgacc ggtgtaacct ttgatcgctc cccgccgtaa tccccctcga cccacccagc
     1201 cgggccggtc acggacatca tcccaaggag ccagtcccat gaaccgcaac accgccaccg
     1261 ccgccctcgc cgccgtcttc gctctgtcgg ccggcgccgc cctcgccgac gacatgaagg
     1321 ccaagaagcc cgccatggaa aagtgctacg gcgtctcgat ggctggcaag aacgactgcg
     1381 ccgcgggcgc tggcacctcc tgcgccggca cctccaaggt cgactaccag ggcaatgcct
     1441 ggacctatgt cgccaagggc acctgcacca ccatcaagac ccccaagggc atgggttcgc
     1501 tgacgcccaa gatgtaatcc ggctctgaca cttgccccct ccgcgctgac gcgctcctcc
     1561 ccctagggga aggacgaccg cgaagcggtc aggagggggc aagtgatcgt ccaactcaag
     1621 agactccccc atgacccccg ccgcaggcct tggcctgaaa cctcagcact atgacgaggc
     1681 tctggccgcg acggcgccgg gcctgtggtt cgaggttcat cccgagaact acatgtcggc
     1741 cggcggaccg cgcctgaccg tcctgacggc gatccgcacg cggcatccgc tgtcgctgca
     1801 tggggtgggc ctgtccctgg ccgccgacgc cgatccggac cccgaacatc tgcaggcgct
     1861 gaagaccctg gtcgatcgct tcgagccgtt cgtggtctct gaacatctgg cctggtcgac
     1921 ccatcgcggc ctgcaccagc ccgaccttct gccctttccc cgcacacacg ccgccttgtc
     1981 acgcatcgcc ggcaatatcg gccggatgca ggacgccctg ggccgccagg ttctggtcga
     2041 gaacccctag ctctacatcc ccctgaccgg ccacgatctg gacgagaccg acttcctcgc
     2101 cgccctggtc cggacgaccg gctgcggcct gctggtcgac atcaataacg tcttcgtcag
     2161 cgcccataac ctcggctacg acgccgccgc ctatctggac gccctgcccg ccgccgtgat
     2221 cggcgaaatc cacctggccg gccatgctcg ggacgagggc ggctcgtccc tgctgatcga
     2281 cacccatggc gcgccagtgg ccgaaggcgt ctggagcctc tatcgccatc taatggcccg
     2341 catcggcccg cgccccaccc taatcgagcg cgacgaccac gtgccggcct tcgcgaccct
     2401 gatggccgag cgcgaccgag cccaggctga actccaacgt ctggagccgg cccatgtctg
     2461 acctgctgga gttccaggac gcctttgtcg aggccctggc cggcgggccg acggtcctgt
     2521 cgccgtggtt ggccgcggcc gacgccggcg agcctggcct gtcagtctat cgcaacacca
     2581 tcgccagggg ctgcgtcgac gccctggccg caaatttccc gaccgtcgcg accctggtcg
     2641 gcgaggactg gttcccgccg ccgccgccct gttcgcccgc gaggccccgc ccacccacgc
     2701 ggccctgctg gactatggcg agaacttccc cgcctggctg gaccgcttcc cgccggccca
     2761 ggacctgccc tatctggcgg gcgtcgccca tctggaccgc ctttggaccg aggccctgtt
     2821 cgccggcgag gcgccgtccc tgcccgccga agccttcagc gaactggcgc ccgagaccct
     2881 ggcccagtcc cgggtgcggc tgcatccgtc cgtccgcttc gccgccttcg gggcgggcct
     2941 gcccagcctg tgggccggcg ctcgcgaggg ccgcgacgac ctcgaactct tcgaaaccgc
     3001 cgagggcctg ctgctcagcc ggcccgacgc gttagtggct taccgcatcg tcggccccgc
     3061 cgactgcgcc tttctcgccg cgtgccggat gggccgcccc ctcgccgagg ccatgggcct
     3121 ggcggcggag gccgatcccg gcgcggacct gcaaatcctc tttgccgccc tgatcgacgc
     3181 caagatcttc agcggcctcg acacgggacc ccgaaaatga ccaccgccct tctgaagccc
     3241 ttcgaccgcc tcgcagaact ggccggcaag gcgctccctg acgatgtcct gaccctggtg
     3301 gcccgactgg gcgtcgccgg catattctgg ctgtcgggcc gcaccaaggt ggatggcgtg
     3361 ctgaccgtca gcgacggcgc ctatgccctg ttttccgacg agtacgccct gccgctgatc
     3421 ccctccgaca tcgccgcgca catggcgacc tatgccgagc acctgctctc gatcctgctg
     3481 gtgctcggcc tgctgacccg cctttcggcg ctgggcttcc tgggcatgac cctgatcatc
     3541 caggtcttcg tctatcccga cgcctggccc acgcatctga gctgggcggg cctcctgctg
     3601 ctgctggtcg caaagggcgg cggcaggttc agcctggatc gcgcgctcgg gatcaaatga
     3661 acccaagcac gcccggcgtc atcaccagcc gccatagatc ggccggaacc ggtgctccag
     3721 gtcgtcgacc tcgcgctcgg cgtcgcgcag ggcccagtcg gcgcgacggc cctcttcgac
     3781 agcggcgcgg cgctcgctgc gcaggcggtt caggcggtcg cgcagttcgc gcttctgctc
     3841 gtcgttgagc gccgggttgc gcagctcgtc ctcgacgccg cgcgcctcgc gatcccgctt
     3901 ttcggcgcgt ctgtcagcgc tggagcgatc ggacttggcc tcgctcagcc gcgtcgtcgc
     3961 ggcgtgcacc agcttgccat cggcataggc cggtagaaaa tcccgggctg cgcgctcggg
     4021 gcagaccccg gcatagtcgt ggcccagacg gccctcatga aagccgcgct ccggcgtgca
     4081 atagagcctc ag
//
LOCUS       DAOMEO010000137         4087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2613_length_4087_cov_2.634177, whole genome shotgun sequence.
ACCESSION   DAOMEO010000137 DAOMEO010000000
VERSION     DAOMEO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_4087_cov_2.634177"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLH31_04265"
     CDS             complement(<1..426)
                     /locus_tag="PLH31_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HQR88475.1"
                     /translation="MAKTGFVRVDADDARTLLGAAAVAAWSTFADSWNDLGLDGFMAD
                     GGRYRRRRFAAFSAAPGAQAGSVVRKPHQPHYQSRDYNALNGGVQRWFEPVTDTVASH
                     PVTLAVIQAGLDLFHPNTPAPPAAWHVELQAGGGLVWRAP"
     gene            complement(450..839)
                     /locus_tag="PLH31_04270"
     CDS             complement(450..839)
                     /locus_tag="PLH31_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQR88476.1"
                     /translation="MTLQSAPFTDAEIDALVETFYAKVQQHHRLGPIFQKAIGDDGWP
                     EHLATLKRFWAGVLQGAGGYKGSPMAVHQRVEGITEGLFTPWLGLFHLTCAELFDAPR
                     AALVGAKAERIARTLKRGLSFRPEVLA"
     gene            946..2052
                     /locus_tag="PLH31_04275"
     CDS             946..2052
                     /locus_tag="PLH31_04275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR88477.1"
                     /translation="MIKLPPLKPDSDQMLHLDALRIVGAVMIVVFHFNRFINLDGRWQ
                     LADDTIKTFSLIVDLFFVISGYVMAAIYTGRLTTPAKYGDFLKKRVARLGPLHWATML
                     VFIAVAAAGLAGWIQDRDPKRYDAACIVPNILFIHAWGVCRSQTWNFVSWAISAEMGM
                     YVALPLIFWITAKGRWVTGGLAILSLVILLQTPHGDRPFHQWTWDFGVLRAVPGFLIG
                     MLGFQIRPLLAKLPYPNLVMWGLLAVFLIASPLEVPRDVLLLVMYGVGLAGIAADAAG
                     PVGPVTRRLAPWAQLSFTLYMLHPIALKMGLAWVGLGMWNLNGDLMRLWVLFWVVALI
                     PLAYLSLILFERPARDWIANLSWPRSDPPTPEAR"
     gene            complement(2009..>2111)
                     /locus_tag="PLH31_04280"
     CDS             complement(2009..>2111)
                     /locus_tag="PLH31_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR88478.1"
                     /translation="AHEAAQAPADHPRYVELDAISELPAWVDRIAAS"
     assembly_gap    2112..2121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2122..2728)
                     /locus_tag="PLH31_04285"
     CDS             complement(<2122..2728)
                     /locus_tag="PLH31_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQR88479.1"
                     /translation="MRTPSKTAITTVGVDADDTLWHCESLFRLTHKRFVDLLAEHGDE
                     ERINAQLAATEKRNLRLYGYGAKGFTLSMIETAMELTDGEVSTRVIREILAAGREMLT
                     EPVEPLPGVEHALAELSKRYRLILITKGDLLHQEQKLASSGLGDLFAAVEIVSEKDAD
                     TYRRVFERHGTGADQAVMAGNSMRSDILPALEAGSWGALIPY"
     gene            2848..4008
                     /locus_tag="PLH31_04290"
     CDS             2848..4008
                     /locus_tag="PLH31_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HQR88480.1"
                     /translation="MAFGDYQNEIYFGGLMRGVKPTLPVDFKSLEARARAALPPATLT
                     YVQGGCGDEFTQDHNAEAFKRWGIVPRMMVDCTTRDLSIDLFGLKLPSPIFMAPIGVI
                     GLCAQDGHGDIATARAAAATGVPMVASTLMNDPMEQVAPAMGDTPGFFQLYTPRDKDV
                     AESLVRRAEAAGFKAIVVTLDTWVTGWRPRDLNDANFPQLRGHALANYMSDPAFRALL
                     AKPPEEDPQAAVAQWGRTFGKALTWADLPWLRSMTKLPLILKGICHPDDARRAIDGGV
                     DGIYCSNHGGRQANGGLAAIDMLKGVVEASGTTPVMFDSGVRSGSDVVKALALGAAAV
                     GVGRPYAYGLALDGVAGIVHVLRSILAEADLLMAVDGYPTLADLRAAGLVRS"
BASE COUNT          614 a         1374 c         1390 g          699 t
ORIGIN      
        1 cggcgcccgc cagacaaggc cgccgccagc ctgcagctcc acatgccagg ccgccggcgg
       61 ggcgggggtg ttgggatgga acaggtccag tcccgcctgg atcaccgcca gggtgaccgg
      121 atggctggcc acggtgtcgg tcaccggctc gaaccagcgc tgcacgccgc cgttcagcgc
      181 gttgtagtcg cggctctgat agtgcggctg gtgaggcttg cggaccaccg atcccgcctg
      241 cgcccccggc gccgccgaga aggccgcgaa gcgccggcgg cgatagcgac cgccgtcggc
      301 catgaagccg tccagtccca ggtcgttcca gctgtcggcg aaggtgctcc acgcggcgac
      361 cgcggcggcg cccagcaggg ttctggcgtc gtcggcgtcg acgcggacaa agccggtctt
      421 ggccagggtc tggctggccc cggtggcgct caagccagga cctcgggccg gaacgacagg
      481 ccacgcttga gggtgcgggc gatgcgctcg gccttggcgc cgaccagggc ggcgcgcggc
      541 gcgtcgaaca gctcggcgca ggtcagatgg aacaggccca gccacggggt gaacaggccc
      601 tcggtgatgc cttcgaccct ttggtgaacg gccatgggcg agcccttgta cccgcccgcg
      661 ccttgcaaca ccccggccca gaagcgcttg agggtcgcca ggtgctcggg ccagccgtcg
      721 tcgccgatcg ccttctggaa gatcggtccc aggcggtgat gctgctgaac cttggcgtag
      781 aaggtctcga ccagggcgtc gatctcggcg tcagtgaacg gagcggattg cagggtcatc
      841 agccctagat ggtgacggag agtcgcaagc caaaccatga cctgaatcaa acccgcccgc
      901 tggctccaac ttgaccgttt cggcgccccg gcccatggca tggccatgat caaactgccg
      961 ccgctgaagc ccgattccga ccagatgctg catctggacg ccctgcgcat cgtcggggcc
     1021 gtgatgatcg tggtgttcca cttcaaccgc ttcatcaatc tggatggccg gtggcagctg
     1081 gccgacgaca cgatcaagac cttcagcctg atcgtcgacc tgttcttcgt gatcagcggc
     1141 tatgtaatgg cggcgatcta taccggccgc ctgaccacgc cagccaagta cggcgacttc
     1201 ctgaagaagc gggtggcccg gctggggccg ctacactggg ccacgatgct ggtgttcatc
     1261 gccgtggccg ccgccggcct ggccggctgg atccaggacc gcgatcccaa gcgctatgac
     1321 gccgcctgca tcgtgcccaa tatcctgttc atccacgcct ggggcgtgtg ccggtcccag
     1381 acctggaact tcgtcagctg ggcgatcagc gccgagatgg ggatgtatgt cgccctgccg
     1441 ctgatcttct ggatcacggc caagggacgc tgggtgaccg gcgggctggc gatcctcagc
     1501 cttgtgatcc tgctgcagac cccgcacggc gaccggccgt tccaccagtg gacctgggat
     1561 tttggcgtgc tgcgcgccgt gcctggcttc ctgatcggca tgctgggctt ccagatccgg
     1621 ccgctgctgg ccaaactgcc gtatccgaac cttgtgatgt ggggcctgct ggccgtcttc
     1681 ctgatcgcct cgccgctgga ggtcccccgg gacgtgctgc tgctggtgat gtacggcgta
     1741 ggcttggccg gcatcgccgc tgatgcggcc ggaccggtcg ggccggtgac ccgccgcctg
     1801 gcgccctggg cgcagctcag cttcaccctc tacatgctgc accccatcgc cctgaagatg
     1861 ggcctggcct gggtggggct ggggatgtgg aacctcaatg gcgacctgat gcgcctgtgg
     1921 gtgctgttct gggtcgtggc cctgatcccg ctggcctatc tgtcgctgat cctcttcgag
     1981 cgcccggccc gcgactggat cgcgaacctc agctggccgc gatccgatcc acccacgccg
     2041 gaagctcgct gatcgcgtcc agctcgacat agcgcgggtg atcggccggc gcctgagccg
     2101 cctcgtgcgc cnnnnnnnnn ngatagggta tcagcgcgcc ccaggaccct gcctccagcg
     2161 ccggtaggat gtccgaccgc atcgaattgc cggccatcac cgcctgatcg gcccccgtgc
     2221 catggcgctc gaatacccgg cgataggtat cggcgtcctt ctccgagacg atctcgaccg
     2281 ccgcgaacag gtcgcccagc cccgatgagg ccagcttctg ctcctggtgc agcagatcac
     2341 ccttggtgat caggatcagg cggtagcgct ttgagagttc cgccagggcg tgctcgacgc
     2401 cgggcagggg ctcgaccggc tcggtcagca tctcgcggcc ggcggccagg atctcgcgga
     2461 tcacgcgcgt cgagacctcg ccatcggtca gctccatggc ggtctcgatc atcgacaggg
     2521 tgaagccctt ggccccgtag ccatagagcc gcagattgcg cttctcggtc gccgcaagct
     2581 gggcgttgat gcgctcttcg tcgccatgct cggccagcag gtcgacgaac cgcttgtggg
     2641 tcaggcgaaa caggctctcg caatgccaga gggtgtcgtc ggcgtcgacg ccgacggtgg
     2701 tgatggcggt cttggacggg gtgcgcatcc ccttcccgtc gccggatcgc ctgcgggcgt
     2761 caagccgcct gacgggatct agcccttgca tcgcgtctga tcgctgttca gtttgcttcc
     2821 aaagattaga aaagacggga ggcgttcatg gcgttcggcg actatcagaa cgaaatctat
     2881 ttcggcggcc tgatgcgcgg cgtgaaaccg accctgccgg tggatttcaa gagccttgaa
     2941 gcccgggccc gcgccgccct gccccccgcg accctgacct atgtgcaggg cggttgcggc
     3001 gacgagttca cccaggatca caacgccgag gccttcaagc gctggggtat tgtgccgcgc
     3061 atgatggtcg actgcaccac gcgcgacctg tccatcgacc tgtttggcct caagctgccc
     3121 agcccgatct tcatggctcc catcggggtg atcggcctgt gcgcccagga tggacacggc
     3181 gatatcgcca ccgccagggc cgccgccgcg acaggcgtgc cgatggtggc ctcgaccctg
     3241 atgaacgatc caatggagca ggtggcgccg gccatgggcg acacgcccgg cttcttccag
     3301 ctctatacgc ccagggacaa ggacgtcgcc gagagcctgg tgcgacgggc cgaggcggcg
     3361 ggcttcaagg ccatcgtcgt gaccctggac acctgggtca ccggctggcg gccgcgcgat
     3421 ctgaatgacg ccaattttcc gcagctgcgc ggccacgccc tggccaacta catgtccgac
     3481 ccggccttcc gcgccctgtt ggccaagccg ccggaagagg acccgcaggc cgccgtcgcc
     3541 caatgggggc gcaccttcgg caaggccctg acctgggctg atctgccctg gctgcggtcc
     3601 atgaccaagc tgccgctgat cctgaagggc atctgccatc ccgacgatgc gcgacgggcc
     3661 atcgacggcg gcgtggacgg aatctattgc tccaaccacg gcggtcggca ggccaatggt
     3721 gggctggcgg cgatcgacat gctgaagggc gtggtcgagg cctcgggcac gacgccggtg
     3781 atgttcgatt ccggggtgcg gtcgggcagc gacgtggtca aggccctggc cctgggcgcg
     3841 gcggcggtgg gcgtgggccg gccctatgcc tatggcctgg cgctggatgg ggtggcgggg
     3901 atcgtccacg tcctgcgcag catcctggcc gaggctgatc tgctgatggc ggtcgacggc
     3961 taccccaccc tggctgacct gcgcgcggcg ggcctggtca ggagctgacg gtcctagaag
     4021 gactggacga cgaagatcgt ggcgctgatc ggcagggcgg ccaccaggat caggcgcagc
     4081 gccgctt
//
LOCUS       DAOMEO010000138         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2625_length_4068_cov_3.279591, whole genome shotgun sequence.
ACCESSION   DAOMEO010000138 DAOMEO010000000
VERSION     DAOMEO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2625_length_4068_cov_3.279591"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLH31_04295"
     CDS             complement(<1..240)
                     /locus_tag="PLH31_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88481.1"
                     /translation="MTSKQHPPERDQRHPGRRLPSKCASTPCCELENPARAARPDHPI
                     VAEIFDALARTMTNNDFGKQRRLVEFKDGCRYGKFG"
     gene            complement(572..1963)
                     /locus_tag="PLH31_04300"
     CDS             complement(572..1963)
                     /locus_tag="PLH31_04300"
                     /inference="COORDINATES: protein motif:HMM:NF020007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQR88482.1"
                     /translation="MGFVIYDLETTGLNKRFDQILQFAAVHTDANLVVQEQVEFEGRL
                     RPHILPSPGALHVTGGHYANLIDPLRPSYDAMLGDIYGTMKRWTPSMFVGFNSISYDE
                     EVLRQAFYESLRAPIYVTNRDGNSRGDLLKLARVVGTLQPDVLKTTRGQDGRRVFRLA
                     DLARANGVHPGRSHTASGDVATTLALCRLIAAEAPILWSSFMRFSNKAEVLSFIAERD
                     AFSVFEFKRGVQDVHPVTAIGFKPADKNVRYCLDLTADLGALRRMTPAELMIEIGKPD
                     GPILRMKANGSPILAELWELDQGLLGDFTEADLETTAANVRGDTAFVQRLVAAAIASE
                     RVWPKSKYVEFQIYEGFIQDWDEDVCRRFHAAAWRDRPALIDRFQDPRLKKLARRLIY
                     FERPDLLDPDIRGQIDRAIWGRLRGNEPDAPGRSYAATLQELDALVAEIQIVSPSLAS
                     YRDHLQAQLVAAA"
     gene            complement(1976..2488)
                     /locus_tag="PLH31_04305"
     CDS             complement(1976..2488)
                     /locus_tag="PLH31_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQR88483.1"
                     /translation="MADKVKARAEALRLFKANAVKSAPVPLDRIARAAGAIVQYAPFD
                     GELSGMAFIKNGQAMIGVNSLHAPTRKRFTLAHELGHVILHRHVLETEGVHVDKGILR
                     RDTLASQGTDDREIEANNFAAELLIPEPLLAAALDGRTLDLEDDEAVQALAKRFNVSA
                     SALVYRIQRS"
     gene            complement(2491..2817)
                     /locus_tag="PLH31_04310"
     CDS             complement(2491..2817)
                     /locus_tag="PLH31_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR88484.1"
                     /translation="MDDMEIYRQLGQNVARVRGKLGKTQAEIASQIGLTRASLANIET
                     GRQKVLMHHVFRLANALECPSVLDLIPETFVFSDDEQPITTHGSLLSDQQHAAVQRFV
                     QIAGKR"
     gene            2942..>3655
                     /locus_tag="PLH31_04315"
     CDS             2942..>3655
                     /locus_tag="PLH31_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HQR88485.1"
                     /translation="MSRVGIAALSQPRRGIHPMTQLIHQEESALNRDAAGVFSVELGD
                     ETFAFRRVRFDDRKVVGLQIAEAAGAHPIEAFSILRHLRSGELESIRPSEPVDLGEEG
                     VERFFVIRGDDLHRFTVEGLSMEWPKAKLATKNIKFLAKASGSQVLVLDSEDGEVVLD
                     DDDLVNLAAKGVERLMLRHPPKTVTIYYKEAPFELERGAYTTEQLIAVFSAPAGYLLD
                     LIERDGEFRELKPGESIKIR"
     assembly_gap    3656..3665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3666..4032
                     /locus_tag="PLH31_04320"
     CDS             <3666..4032
                     /locus_tag="PLH31_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88486.1"
                     /translation="VLVAPAGGTLVVRDPASELRKLRRICRRAELHEDGGQRLVHLPE
                     LRVQYAGGVETVDGLLCPGQHSGYVSRLFLSKPFPARGQNWTTHNILGALWYAMSWNG
                     VDPSLGWDEILGAHLRPLQ"
BASE COUNT          727 a         1261 c         1289 g          781 t
ORIGIN      
        1 tccgaatttt ccatagcggc aaccgtcctt gaactcgacg agacgacgct gcttgccgaa
       61 gtcattgtta gtcatggttc gcgcaagtgc gtcgaaaatt tctgccacga tcggatgatc
      121 aggccgagcg gcacgagcgg gattttccag ctcgcaacaa ggcgttgaag cgcacttgct
      181 ggggagacgg cgtccggggt gacgctgatc gcgctccggc ggatgttgtt ttgacgtcat
      241 cgacacagct cccatcgcct agatttgtcg atctacgtct tcgacggtta ccatgccaag
      301 cgcctgtgat gccggcgcta actccagcgt ggtgtggagt gcaattcgga cgtatcagtc
      361 gcttatcgcg taatcattgc gcgagccgct ctccaggttt tgtcatcatc ttcatacgca
      421 cccgcatgcc gtagctctca acctgctagc cgccctgcag ctttggcggc tacaggatgc
      481 tcggaccgag gtcgggatcc tgtgcaagct ctggccgctt tgcacgcccc agccggtcag
      541 agcaagatta gtgcgagtcg gccacacgac ctcaggcggc cgcgacgagc tgcgcctgaa
      601 gatggtcccg gtagctcgcc agggacgggc tgacgatctg gatctcggcc accaaggcgt
      661 ccaattcctg cagcgtggcg gcgtagcttc ggcccggggc gtcgggctca tttcccctca
      721 agcgccccca gatcgcccgg tcgatctgtc cgcgaatgtc agggtccaga agatcaggtc
      781 gctcgaaata gattaatcgc cgggcgagct tcttcagccg cggatcttgg aagcggtcaa
      841 ttagcgctgg ccgatccctc caggcggcgg catggaatcg cctgcatacg tcctcgtccc
      901 agtcctggat gaagccctcg tagatttgaa actcgacgta cttggacttc ggccagaccc
      961 tttcggaggc gatggccgcg gccaccaacc gctgaacgaa cgccgtgtcg ccgcgcacat
     1021 tggcggccgt cgtctccagg tcagcctcgg tgaagtcgcc gagtagccct tggtctagtt
     1081 cccacagttc ggccaagatc ggcgagccgt tggccttcat gcgcaggatc ggtccgtcag
     1141 gcttgccaat ctcgatcatc agctccgccg gcgtcatccg ccgcaacgcg ccaaggtccg
     1201 ccgtcaggtc caggcagtag cggacgttct tgtcggcggg cttgaagccg atcgcggtca
     1261 ctggatgaac gtcctgcacg cctcgcttga actcgaacac cgagaacgca tcacgttcgg
     1321 cgatgaagct cagaacctcg gccttgtttg agaaccgcat gaagctcgac cagaggatcg
     1381 gcgcttcagc ggcgatcagg cggcaaagcg ccagggtggt cgcgacatcg cccgacgcgg
     1441 tgtgcgatcg accgggatgg acgccgttgg cccgggcgag gtcggccagg cggaacacgc
     1501 gccggccgtc ctgaccccgg gtggtcttaa gaacgtcggg ctgcagggtc ccgaccaccc
     1561 gcgccagctt aagcaggtcg ccgcgcgaat tgccgtctct gttggtgacg tagatcggcg
     1621 cccggaggga ctcgtagaag gcttggcgca ggacctcttc gtcgtagctg atggagttga
     1681 agccaacgaa catcgacggg gtccaacgct tcatcgtccc atagatgtcg cccagcatgg
     1741 cgtcgtagga cggccgcaac gggtcgatca gattcgcgta gtggccgccc gtgacatgca
     1801 gggcgccggg cgaaggcagg atgtgggggc gaaggcgtcc ctcgaattcg acctgctcct
     1861 gcaccacaag gttcgcgtcg gtgtggaccg cggcgaattg caggatctgg tcgaaccgtt
     1921 tgttgaggcc cgtggtctcg aggtcataga tcacgaagcc catcgacccg cccgcctaag
     1981 accgttggat ccggtagacc agggcgctgg cgctgacgtt gaagcgcttg gccaaggcct
     2041 gcaccgcctc gtcgtcttca aggtcgaggg ttcgcccatc gagcgcggcg gcgagcagag
     2101 gttctggaat taagagttct gcggcgaagt tgttcgcctc gatctcccgg tcgtccgtgc
     2161 cttgggacgc cagagtgtcg cggcgaagga tgcccttgtc gacatggacg ccctccgtct
     2221 ccaacacatg acggtggagg atcacgtggc caagttcgtg cgccaaggtg aaccgcttgc
     2281 gggtcggcgc atgaagggag ttcacgccga tcatcgcctg gccattcttg atgaaggcca
     2341 tgccggacag ttcgccgtcg aacggcgcgt attgcacgat ggcgccggcc gcgcgggcga
     2401 tgcggtcgag cggcacgggc gcggatttga cggcgttggc cttgaagagc cgcaaggctt
     2461 ccgccctggc tttcacctta tcggccatgg tcagcgcttt ccggcgatct ggacgaaccg
     2521 ctgcaccgcg gcgtgctgct ggtcactgag cagacttccg tgcgtggtga tcggctgctc
     2581 gtcatcggag aagacgaacg tctctggaat gagatccagg acagacgggc attcaagcgc
     2641 gttggccagc cgaaagacgt ggtgcatcag gaccttctgg cggccggttt cgatattggc
     2701 cagcgacgcg cgagtcaggc cgatctgcga ggcgatctcg gcctgggtct tgccgagctt
     2761 gccgcgcacg cgcgcgacat tctgaccaag ctgccgataa atttccatgt cgtccatggg
     2821 aacataatga caacttcgta gagtcgcgtc aatcaggctg acattttgtg tgcttttcat
     2881 aaacatatct gggttaacat gtgtgtagtg ttgactttat cacgagtggc ggtcattctt
     2941 attgtcacgg gtcggcatcg cggccctttc acaaccaaga cgaggtatcc atcccatgac
     3001 tcagctcatt catcaggagg agagcgccct caaccgagac gccgccggcg ttttctcggt
     3061 cgaactcggc gacgaaacct tcgcgttccg ccgcgtgcgc tttgacgatc gcaaggtcgt
     3121 cggtctccag atcgctgaag ccgccggcgc ccatccgatc gaagcgttct ccatcctccg
     3181 ccatctgcgc tcgggcgagc tggagtcgat ccggccctcc gagcccgtcg atctgggcga
     3241 agagggggtg gagcgctttt tcgtgatccg cggcgacgac ctccaccgct tcacggtgga
     3301 gggcctgtcg atggagtggc cgaaggcgaa gctggccacc aaaaacatca agttcttggc
     3361 aaaggcctcc gggagccaag tgctggtgct cgacagcgag gacggcgaag tcgtcctcga
     3421 cgacgacgat ctggtgaacc tggccgccaa gggcgtggag cggctgatgc tgcgccaccc
     3481 gcccaagacg gtgacgatct actacaagga agcgccgttc gaactggagc gcggcgccta
     3541 caccaccgag cagctgatcg ccgtgttctc ggcgccggcc ggctacctgc tcgacctgat
     3601 cgagcgtgac ggcgagttcc gcgaactgaa gcccggtgag tcaatcaaga tccgcnnnnn
     3661 nnnnnagttc tcgtcgcacc cgccggcggg acactcgtcg tgagggaccc ggcgtctgaa
     3721 ctgcgcaagc tgcgtcggat ctgccgccgg gccgaactcc acgaagatgg cgggcaacgg
     3781 ctcgtccatc ttccggagtt gagggtccag tacgcgggcg gggtggagac ggtcgatggc
     3841 cttctctgcc ccggccagca cagcggctac gtctcgcgcc ttttcctgtc gaagcccttt
     3901 cccgcccgcg gccagaactg gaccacgcac aacatcctcg gcgcgctctg gtacgccatg
     3961 tcgtggaacg gcgtcgatcc cagcctcggc tgggacgaaa tcctgggcgc ccacttgagg
     4021 cctctgcaat gaacgtccac ctgaagatcg acggccggct gctcgacg
//
LOCUS       DAOMEO010000139         4068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2626_length_4068_cov_2.447545, whole genome shotgun sequence.
ACCESSION   DAOMEO010000139 DAOMEO010000000
VERSION     DAOMEO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_4068_cov_2.447545"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLH31_04325"
     CDS             complement(<1..248)
                     /locus_tag="PLH31_04325"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQR88487.1"
                     /translation="MDPAVVDLSRLQFALTALYHFLFVPLTLGLSFMLVIMESIYVMT
                     KRPIWRTVTRFWAGLFGINFVLGVATGLTMEFQFGMNWS"
     gene            372..>873
                     /locus_tag="PLH31_04330"
     CDS             372..>873
                     /locus_tag="PLH31_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HQR88488.1"
                     /translation="MQARPALIAGQNQVTTRLNDVARSGAAPLRLARLLVLLDGAAAI
                     AFAAGLACAIDALAAGREFRGWLALLVVATLARGLMTKLAGEAGARAAAQVKAAARTD
                     AASALAGRKPGSALSSSDATTALVEGVEALDGHVSRFLPARFAAAATPLALIAVAALA
                     SPISAAI"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <884..2033
                     /locus_tag="PLH31_04335"
     CDS             <884..2033
                     /locus_tag="PLH31_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR88489.1"
                     /translation="LGALLPFIIAMALAGTAAAAESRAQFTALERLSGLFLDRVRALP
                     VVLAFQAEQRITRDLARSADELATRTIRVLRVAFFSSGALEFFAALSVALVAVYCGFN
                     LLRLLPFPVPEQLDLKRAFFVLALAPEIYLPMRRLAAAYHDRQAAEAATASLIALPRP
                     QPRPAAATFAAPPAIRFSSVTLRYPDADAPVLEGFDLDVPAGQTVALLGPSGSGKTSL
                     LNLLLDLAPLSAGAVLIDGRPLSELGALSGQIAWAGQHPLITPGSIADNIALAHRVAT
                     RAQIEAAARRVGLAGALAMRPGGLDAWLDERGSGLSGGERRRLSLARALLKPAPILLM
                     DEPTANLDAVAETELLDAIREAARGRTTLIATHSQAVADLADHVVRLT"
     gene            2030..3613
                     /locus_tag="PLH31_04340"
     CDS             2030..3613
                     /locus_tag="PLH31_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR88490.1"
                     /translation="MSASPLQTLIRVQRRRHAEGLLLAAVCAAVVGAGSTVLLGLSGW
                     FITGAALAGAVGLGAAQAFNVLLPGATIRLLAILRTGARYGERLSGHAAALRALAAIR
                     PVLFSSLAAAPPAEALALSGGEASARLVQDVDAVETRFVRLSAPWGAGAAVAAGVALG
                     LLAGWAAGLAILASAFAAILVARALAQRLSAPAGRQIQIRMGGLKDAAASLAAAAPEL
                     RAYGLETWASETLMAHGQTVDAARQDAARARGWIAASQAVILGLATAAAVALAAPAGA
                     ALAALAGLAAAMAVDGVGNLAAAFDQDGASTEAARRLDAILRHTPATPAAAAPQEPDL
                     GLLGETFAPGACIAIIGPSGVGKTTLLERLVALRPSPRGAITVGGADLADLRPAVARA
                     VFAHAPQDAALIAGTVADNLRLAAPDASEDDLWAALRDAALDERIRAAPAGLTAWLGD
                     NGERLSGGERRRLSLARAYLRPAPWLLLDEPTEGLDAATEALVLQRLATRLDRTGQGL
                     VLVSHRPAPLTICDRKLSL"
     gene            3727..>4068
                     /locus_tag="PLH31_04345"
     CDS             3727..>4068
                     /locus_tag="PLH31_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQR88491.1"
                     /translation="MTAARPTSYPLFSDPRPGPYMVAAVAVAATFVVRLSLGEAFTAA
                     SSFMLFIPAVLVSAALGGLICGLFATVCASLVVGALSHNDAQTLVGALSGVIFLLVGI
                     GMAIGGGWFHAA"
BASE COUNT          506 a         1524 c         1402 g          626 t
ORIGIN      
        1 gaccagttca tgccgaactg gaattccatg gtcaggccgg tggccacgcc caggacgaag
       61 ttgatgccaa acagcccggc ccagaaccgc gtgaccgttc gccagattgg gcgcttggtc
      121 atcacgtaga tgctctccat gatgacgagc atgaacgaga gccccagggt cagaggcacg
      181 aacaggaagt ggtacagcgc ggtcagcgcg aactgcagcc tcgagagatc gacgacagcg
      241 gggtccatgc acagctccga aacggacggc gaatgatcac gccagccgag agcactgccc
      301 tagaccggtc gaaccctgcg ggccttgata aaaatcaagg cgcgaaggcg acgccatcgt
      361 acactcaacc catgcaagct cgtccagcgc ttatcgctgg ccaaaaccag gtcacgacaa
      421 gactgaacga cgtcgcgcgg tccggcgcag cgcccctgcg cctggcgcgt ctgctggtgc
      481 tgctggacgg cgcagccgcg atcgccttcg ctgccgggct ggcctgcgcc atcgacgccc
      541 tggccgccgg gcgagaattc cggggatggc tggcgctgct ggtcgtcgcg accctcgcgc
      601 gcggcctgat gaccaagctg gcgggagagg ccggcgcgcg ggcagccgcc caggtgaaag
      661 ccgctgctcg aaccgacgcc gcctccgccc tggccggccg caaacccggc agcgccctgt
      721 cgagcagcga tgcgacaacc gctctggttg aaggggtcga ggcgctggat ggccatgtct
      781 cgcgcttcct gccggcccgg ttcgcggcgg ccgcgacgcc cctggccctg atcgccgtgg
      841 ccgccctggc cagtcccatc tccgccgcca tccnnnnnnn nnncctgggc gccctgctac
      901 ccttcatcat cgccatggct ctggctggga cggcggcggc cgccgaatcc cgcgcccagt
      961 tcacggcgct ggagcgactg tcgggtctgt tcctcgaccg tgtccgcgcc ctgcccgtgg
     1021 tgctggcctt ccaggccgag cagcggatca cccgcgacct cgcccgctcg gccgacgagc
     1081 tggcgacccg tacgatccgc gtcctgagag tcgccttctt ttcgtcaggc gcgctggagt
     1141 ttttcgccgc cctctctgtg gctctggtcg cggtctattg cggcttcaac ctgctgcgcc
     1201 tgctgccctt cccggttccc gagcagctgg atctcaagcg cgccttcttc gtgctggccc
     1261 tggcgccgga gatctatctg ccgatgcgcc gcctggccgc cgcctatcac gatcgccagg
     1321 ccgccgaggc cgcgaccgcc agcctgatcg ccctgccccg cccgcaaccg cgtccggcgg
     1381 ccgcgacctt cgccgccccg ccggccatcc ggttcagcag cgtaaccctc cgctatccgg
     1441 acgccgacgc cccggtgctg gagggcttcg acctggacgt tccggctgga cagaccgtcg
     1501 ccctgctggg ccccagcggc tcgggcaaga cctcgctgct gaacctcctg ctcgatctgg
     1561 ccccgctgag cgccggggcc gtgctgatcg atggccggcc gctgagcgag ctgggcgcgc
     1621 tgtcgggaca gatcgcatgg gctgggcaac atccgctgat cacgccgggc tccatcgccg
     1681 acaatatcgc cctggcccat cgcgtcgcga cccgcgccca gatagaggcc gccgcccggc
     1741 gggtgggtct ggccggcgcc ctggccatgc ggcctggcgg cctagacgcc tggctggacg
     1801 agcgcggctc tggcctgtcg ggcggtgaaa ggcggcggct gtccctggcc cgcgccctcc
     1861 tcaagcccgc gcccatcctg ctgatggacg agccgaccgc caatctggac gccgtcgcag
     1921 agacagaact tctcgacgcg attcgggaag ctgcgcgggg ccgcaccacc ctgatcgcca
     1981 cgcactccca ggctgtcgcc gatctggccg accatgtggt gcggctgaca tgagcgcctc
     2041 gcccctccag accctgatcc gcgtccaacg ccgccggcat gccgagggcc tgctgctggc
     2101 cgctgtctgc gccgccgtgg ttggtgcggg gtcgaccgtg ttgctgggcc tgtctggctg
     2161 gttcatcacc ggggccgctc tggcgggggc ggtaggtctt ggggcggccc aggccttcaa
     2221 cgtgctgctg ccgggagcga ccatccggct gctggccatc ttgcgaaccg gcgcgcgcta
     2281 tggcgaacga ttgagcggcc atgccgccgc cctgcgcgcc ctggcggcga tccggccggt
     2341 gctcttttct tcccttgccg ccgctccgcc cgccgaggcc ctggccctgt ccggcggcga
     2401 agcctccgcc cggctggtgc aggatgtcga cgcggtcgag acccgcttcg tgcggctttc
     2461 ggcgccgtgg ggcgcgggcg ccgccgtggc cgccggtgtg gcgcttggcc tgctggcggg
     2521 ctgggcggct ggtctcgcga ttctggcgtc ggccttcgcc gcgatccttg tggcgcgcgc
     2581 cctggcccag cgcctctccg ccccggccgg ccgccagatc cagatccgca tggggggcct
     2641 caaggacgcc gccgccagcc tcgccgccgc agcccccgag ctccgcgcct atggactgga
     2701 aacctgggcg agtgaaaccc tgatggcgca tggccagact gtggacgccg cccgtcagga
     2761 cgccgcccgc gccaggggct ggatcgccgc ctcgcaggct gtgatcctgg ggttggcgac
     2821 ggccgccgcc gtggccctcg ccgcgccggc gggagcggcc ctggcggcgc tggcggggct
     2881 ggccgccgcc atggccgtag acggcgtcgg caacctggcg gcagcgttcg accaggacgg
     2941 cgcatccacc gaagccgccc gccgcctcga cgccatcctg cgccacacgc cggcgacccc
     3001 ggcagccgcc gcgccgcagg agcctgacct tggcctgctg ggcgagactt tcgcccccgg
     3061 ggcttgcatc gccatcatcg gtccctcggg cgtgggcaag accacgctgc tggaacgcct
     3121 ggtggccctg cgccccagcc cgcgaggcgc gatcaccgtg ggcggggccg acctggccga
     3181 cctgcgcccc gccgtcgccc gcgcggtctt cgcccatgcg ccccaggatg cggccctgat
     3241 cgccggcacg gtggccgaca atctgcgtct cgccgccccc gatgcgtccg aggacgacct
     3301 gtgggccgcc ctgcgcgacg ccgcgctgga cgagcgtatc cgcgccgcgc cggccggcct
     3361 gacggcctgg ctgggcgaca atggcgagcg cctctccggc ggcgaacggc ggcggctgag
     3421 cctggcccgc gcctatctgc gtcctgcccc ctggctgctt ctcgacgagc cgaccgaggg
     3481 tctggacgcc gcgaccgagg cgctggtgct gcagcgcctg gccacgcgtc tggatcgcac
     3541 cggccagggc ctggtgcttg tcagccatcg gcccgcccca ctgacgatct gtgatcgaaa
     3601 gctgtcgctg tagaccatga tgagaggtct ccccgcggga gacggccgcg acgaccagcg
     3661 ggcgcggggc cgtgcaatgc gaccggcttg ggctataagg cgcgaggcaa cagagacgaa
     3721 gacaccatga cggcggcgcg gccgacctcc tatcccctgt tcagcgatcc gcgtcccggc
     3781 ccctatatgg tggccgccgt ggccgtggcg gccacgttcg tggtgcggct gtcgctgggc
     3841 gaggccttca ccgcggcctc gtcgttcatg ctgttcatcc cggcggtcct ggtcagcgcc
     3901 gccctgggcg gcctgatctg cggcctgttc gcgacggtct gcgcgagcct ggtggtcggg
     3961 gccttgagcc acaacgacgc ccagaccctg gtcggggcgc tgagcggcgt gatcttcctg
     4021 ctggttggca tcggcatggc cattggcggc ggctggttcc atgccgcg
//
LOCUS       DAOMEO010000140         4066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2627_length_4066_cov_2.283720, whole genome shotgun sequence.
ACCESSION   DAOMEO010000140 DAOMEO010000000
VERSION     DAOMEO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_4066_cov_2.283720"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLH31_04350"
     CDS             <1..592
                     /locus_tag="PLH31_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HQR88492.1"
                     /translation="PWMPFSLNQPLGLATAGKLSQIAADPVACRAALSEGGVKFTDQP
                     VREKDFCSTRDTVRFPSRLAPAAPVMTCPMALSYVLWERQVLAPAAREILDARVIRVD
                     HFGTYACRTLYGRPGERPSQHARANALDGAGFQLSDGRKLSIIGDFRDPGPEGRFLRA
                     ARDGACKLFSVVLSPAYNAAHADHLHLDHSPYPLCR"
     gene            663..1415
                     /locus_tag="PLH31_04355"
     CDS             663..1415
                     /locus_tag="PLH31_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HQR88493.1"
                     /translation="MAYRVPRHPRIEHHAKHQTRLARLTGLILLWILVAIMALLAERT
                     IPSQHAPWKALRVVDPVGMATRIKAARSGQDPKLCRAILAKAGLTYRDLPDRTEGDFC
                     VVRDALQIESGMAPLLPADAPMVCKEALALSIWERQVVQPAAYELLGQGVTGLEHYGA
                     YSCRRMYGKPDGPVSEHALANAVDLAGFKLTDGSRVMVATDWTDPGPKGQFLRRVRDG
                     ACKVFLTTLSPDYNAEHRDHFHLDMSGWNKCA"
     gene            complement(1420..1719)
                     /locus_tag="PLH31_04360"
     CDS             complement(1420..1719)
                     /locus_tag="PLH31_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88494.1"
                     /translation="MPHTVDADTALEDVLGFLENPPEPGSPQDAIFGQRLLQVISASI
                     PADPENEDDEPPALELGADLRRKLEDAARKRGGGHPFGDHPDGIGPTLGMDLGKR"
     gene            complement(1773..2255)
                     /locus_tag="PLH31_04365"
     CDS             complement(1773..2255)
                     /locus_tag="PLH31_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQR88495.1"
                     /translation="MPLDTANIDQDRDLVLTRIIDVPAAKLFRCWTTPELLPKWFCPP
                     PWYVSKAVIDVRTGGNSLITMNGPDGEVVDNPGVYLEVIENQKIVFTDAFTETWKPSA
                     KPFMTGIITFEDLGDGRTRYTAIARHWTVEDRQTHEQMGFHEGWGVAADQMAALAVTL
                     "
     gene            2420..3184
                     /locus_tag="PLH31_04370"
     CDS             2420..3184
                     /locus_tag="PLH31_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR88496.1"
                     /translation="MGLFDLTGKVAIITGSSRGIGKAIAERMAEHGAKVVISSRKAGP
                     CDEVAAEITARYGEGSAISVPANIGSKEDLQRLVDETKKAFGKVDICVCNAASNPYYG
                     PLAGIADDQFRKILDNNIISNHWLIGMVAPEMRERRDGSIIIVSSIGGLRGNAIIGAY
                     NISKAADFQLARNLAHEFGPDNVRVNCIAPGLIKTDFAKALWDNPETLERSTKGVPLR
                     RIGEPDELAGAAVYLASQAGSFMTGQALVVDGGATI"
     gene            3260..>4066
                     /locus_tag="PLH31_04375"
     CDS             3260..>4066
                     /locus_tag="PLH31_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR88497.1"
                     /translation="MRRFLASLALIGSLAVCAGAWAADDAPSALAVRLNQLNDQIGAH
                     QVKTPPRDNADIERAGRKALRQTGEERLYGLWRVLYAYKSNQIQGRFDTWIARARLTA
                     ERDRDAPLLALTELMVLAYRNETQGYRAFSDADWKRFLNGPSQDIRMMAGVERARHLG
                     QSGRWAEAARLAADLSLEIERRGDIARPLLAEAHQVHSYTLADIGDKEGALDRMTQAA
                     NLDERDAFYIRKIERVYDIAYTAVEVGEIDAAARLARLHNEMIRANGDPAL"
BASE COUNT          667 a         1388 c         1342 g          669 t
ORIGIN      
        1 gccatggatg ccgttctcgc tgaaccagcc cctgggcctg gcgacagcgg gcaagctgtc
       61 gcagatcgcc gccgacccgg tcgcctgccg cgcggctctt agcgagggcg gcgtgaagtt
      121 caccgaccag ccggtgcgcg agaaggattt ctgctcaacg cgagacaccg tccgcttccc
      181 cagtcgcctg gcgccggccg cgccggtcat gacctgcccg atggcgctca gctatgtgct
      241 ttgggagcgg caggtgctgg ccccggcggc gcgggaaatc ctcgacgccc gcgtcatacg
      301 cgtcgatcac ttcgggacct atgcctgtcg caccctctat ggccgaccgg gcgagcggcc
      361 cagccagcac gcccgcgcca acgccctgga cggcgccggt tttcagctat cggacggtcg
      421 caagctcagc attatcggcg acttccgcga tcccggtccc gagggccgtt tcctgcgcgc
      481 cgcccgcgac ggcgcctgca agctgttcag cgtggtgctg agccccgcct acaacgccgc
      541 ccacgccgac cacctgcatc tcgatcacag tccgtatcct ctgtgccgct gaagcggttt
      601 gccggttgaa tcgctggccc gtgtggtgcg tcgttaacct ttgcaaggcg ctcccccgcc
      661 ccatggccta tcgcgttccg cgccacccgc gcatcgagca ccacgcaaag catcagacgc
      721 gcctggcgcg gctgacaggc ctgatattgc tctggatcct ggtggcgatc atggcgctgc
      781 tcgccgagcg caccattccc agccagcacg cgccctggaa ggcgctgcgg gttgtcgacc
      841 ctgttggcat ggccacgcgc atcaaggccg cccgcagcgg gcaggacccc aagctctgcc
      901 gggcgatcct ggccaaggcg ggcctcacct atcgcgacct gcccgacagg accgagggcg
      961 atttctgcgt cgtccgcgac gccttgcaga tcgaatccgg catggcgccg ctgctgcccg
     1021 ccgacgcccc catggtctgc aaggaggccc tggccctgtc gatctgggag cgtcaggtcg
     1081 tgcagcccgc cgcctatgag cttctgggac agggcgtgac cggcctggag cactacggcg
     1141 cctattcgtg ccggcggatg tacggcaagc ctgacgggcc ggtcagcgag cacgccctgg
     1201 ccaacgcggt cgacctggcc ggtttcaagc tgaccgacgg cagccgcgtg atggtggcga
     1261 ccgactggac cgatcccggc cccaaggggc agttcctgcg ccgggtgcgg gacggcgcct
     1321 gcaaggtgtt cctgaccacg ctgagccccg attacaacgc cgagcaccgc gaccacttcc
     1381 atctggacat gagcggctgg aacaagtgcg cctgaggcgt cagcgcttgc ccagatccat
     1441 acccagggtc gggccgatgc catcggggtg gtcgccaaag gggtgaccgc cgccccgctt
     1501 gcgggcggcg tcctccagct tgcgacgcag gtccgcgccc agctccagcg ccggtggctc
     1561 gtcgtcttcg ttttccggat cggcggggat cgaagcggag atcacctgca gcaaccgctg
     1621 gccaaagatc gcgtcctgcg gcgagcctgg ctcgggcggg ttttcgagaa agcccaggac
     1681 gtcctcaagc gcggtgtcgg catcgacggt gtggggcatg gggaactcct gcatgtgcag
     1741 gagcaacggc gatgacgccg ggctggttcc ccctaaagcg tcaccgccag cgcggccatc
     1801 tgatcggccg caacgcccca gccttcgtgg aaacccatct gctcgtgggt ctggcggtcc
     1861 tcgaccgtcc agtggcgggc gatggcggtg tagcgggtcc tgccgtcgcc caggtcctcg
     1921 aaggtgatga tgccggtcat gaacggcttg gccgacggct tccaggtttc ggtgaaggcg
     1981 tctgtgaaca cgatcttctg gttctcgatc acttccagat agacgcccgg attgtcgacc
     2041 acctcgccgt cggggccgtt catggtgatc aggctgtttc caccggtgcg cacgtcgatc
     2101 acggctttgg acacgtacca gggcggcggg cagaaccact tgggcagcag ctctggcgtg
     2161 gtccagcacc tgaacagctt ggcggccggg acgtcgatga tgcgggtcag gaccaggtcg
     2221 cggtcctgat cgatgttggc ggtgtcgaga ggcatggtcg gctccatcgg aattggcgtg
     2281 actgtcgcct caagggcacg cgggctccag aggcgcagac acagcagaca ggtttatttg
     2341 gcgcggtggc gatcttgacc caaggtccgc ctcaacgcac tgtgaccgaa attcaaacaa
     2401 gcgtttcagg gagcgcggca tgggcctctt cgacctcacc ggcaaggtgg cgatcatcac
     2461 cggatcctcg cgcgggatcg gcaaggccat cgccgagcgc atggccgagc acggcgccaa
     2521 ggtggtgatc tcctcgcgca aggccggacc ctgcgacgag gtggcggccg agatcaccgc
     2581 ccggtacggc gagggctcgg ccatctcggt cccggccaat atcggctcga aggaagacct
     2641 gcagcgcctg gtcgacgaga cgaagaaggc cttcggcaag gtcgacatct gcgtctgcaa
     2701 cgccgccagc aatccctatt acggcccgct ggccgggatc gccgacgacc agtttcgcaa
     2761 gatcctcgac aacaacatca tcagcaatca ctggctgatc ggcatggtcg cgccggagat
     2821 gcgcgagcgc cgggacgggt cgatcatcat cgtcagctcg atcggcggtc tgcgcggcaa
     2881 cgccatcatc ggggcctaca acatcagcaa ggccgccgac ttccagctgg cccgcaacct
     2941 cgcccacgag ttcggcccgg acaatgtgcg ggtcaactgc atcgcgccgg gcctgatcaa
     3001 gaccgacttc gccaaggccc tttgggacaa tcccgaaacc ctggagcgct cgaccaaggg
     3061 cgtgccgctg cgccgcatcg gcgagccgga cgaactggcc ggggcggcgg tctatctggc
     3121 ctcgcaggcg ggcagtttca tgaccggcca ggccctggtg gtggacggcg gcgcgaccat
     3181 ctgatcgccc gtttcggtaa gcttaacata aactcaaccg cttcaaccgc agggtctggg
     3241 cctcacctga ggaccggcct tgcggcgatt tcttgcatcc ctcgccctga tcgggagcct
     3301 ggcggtctgc gccggcgcct gggcggcgga tgatgcgcca tcggccttgg ctgttcgcct
     3361 caatcagctg aacgaccaga tcggcgcgca tcaggtcaag acgcccccgc gtgacaatgc
     3421 cgacatcgag cgcgcgggcc gcaaggcgct gcggcagacc ggcgaggagc ggctctatgg
     3481 tctttggcgg gtgctctacg cctacaagag caaccagatt caggggcgct tcgacacctg
     3541 gatcgcccgc gcccgcctga ccgccgaacg tgaccgcgac gccccgctgc tggcgctgac
     3601 agagctcatg gttctggcct atcgcaacga gactcaaggc tatcgcgcct tttccgacgc
     3661 ggactggaag cgcttcctga acggtccgtc gcaagatatc cggatgatgg ccggggtcga
     3721 gcgggcgcgt catctgggcc agtccgggcg ctgggccgag gccgcgcgcc tggccgctga
     3781 cctgtctctg gagatcgagc ggcgcggcga catcgcccgg ccgctgctgg ccgaagccca
     3841 tcaggtgcat tcctacaccc tggccgatat cggcgacaag gaaggcgcac tggaccgtat
     3901 gacccaggcg gccaatctcg acgaacgcga cgccttctac atccgcaaga tcgagcgggt
     3961 ttatgacatc gcctacaccg ccgtcgaggt cggcgagatc gatgcggcgg cccggctggc
     4021 ccggctgcac aacgagatga tccgcgccaa tggcgatccg gcgctg
//
LOCUS       DAOMEO010000141         4048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2639_length_4048_cov_2.200351, whole genome shotgun sequence.
ACCESSION   DAOMEO010000141 DAOMEO010000000
VERSION     DAOMEO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_4048_cov_2.200351"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PLH31_04380"
     CDS             <1..154
                     /locus_tag="PLH31_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HQR88498.1"
                     /translation="KMSFEMEPRAAGYPTTVVYDKQGRERARLAGDADWSSPEARGLV
                     EKLLAE"
     gene            151..897
                     /locus_tag="PLH31_04385"
     CDS             151..897
                     /locus_tag="PLH31_04385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR88499.1"
                     /translation="MTVGFDIDPAVLEPIADRAWPARERSALGGWRLNATAGWSMRSN
                     ACWPLAEPDRDVETAIEAAEAWFVSRGLPRRFKLTEGATAPADLSQRLSRRGYADCKK
                     TLVMVGPALGRADPAVAFSAAPDASFEAVFTASAGGNVEDARERLETLARIPAPARFA
                     RLDIDSRPAALGACAVGGEWVGLFGMRTAVEHRRKGLARLVLRGLLAEAASLGAVHAY
                     LQVEADNAPAIALYEVEGFRTAYTYRYWVR"
     gene            complement(894..>1364)
                     /locus_tag="PLH31_04390"
     CDS             complement(894..>1364)
                     /locus_tag="PLH31_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQR88500.1"
                     /translation="PAVEEAQRLARGEAFAWRLSLTAAKAATGGFEALTFIEEGAGPE
                     GQTGLICARPDTAGDIVLARKDVGVAYHLAVVLDDALQDITHVIRGEDLFEAAHIQRL
                     LQALLGLPTPTYRHHRLLTGPDGKRYAKRDKAQTLRELRAAGVTPAALRSEMGL"
     assembly_gap    1365..1374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1375..1742)
                     /locus_tag="PLH31_04395"
     CDS             complement(<1375..1742)
                     /locus_tag="PLH31_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQR88501.1"
                     /translation="MGFATRFAPSPTGLLHRGHAFSALTAFEAARAMNGRFVLRIEDI
                     DATRCRPEYETAILEDLAWLGLEWARPVRRQSEHLADYHAAIESLQGRGLVYRCFRTR
                     RELDIGRAPHEPQAPFTGGP"
     gene            complement(1747..>1993)
                     /locus_tag="PLH31_04400"
     CDS             complement(1747..>1993)
                     /locus_tag="PLH31_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR88502.1"
                     /translation="QMKEEGAYCQVRDTISEQSLDGVSDIDLELATIAAWCAGHGLAE
                     MISFKQFDPLKELLGGEDAFLRAVFEHLGMFAKYDRG"
     assembly_gap    1994..2003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2004..2376)
                     /locus_tag="PLH31_04405"
     CDS             complement(<2004..2376)
                     /locus_tag="PLH31_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR88503.1"
                     /translation="MTIPATESRPYHHGDLSRALVDAARRILEREGPAALSLRAVARE
                     AGVSPAAPYHHFKDKGQLLEAVAHEGWMALDTALSQSRASARDARERMTSIGVTYVQF
                     ARDNPALYRVMYDCSRDKDALP"
     gene            2534..2644
                     /locus_tag="PLH31_04410"
     CDS             2534..2644
                     /locus_tag="PLH31_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88504.1"
                     /translation="MLRSLTAFEAAFDRMAGGYFLVLALAVAGAVAGVVL"
     gene            2856..>2927
                     /locus_tag="PLH31_04415"
     CDS             2856..>2927
                     /locus_tag="PLH31_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517658.1"
                     /note="activates RNA polymerase to cleave back-tracked RNA
                     during elongational pausing; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HQR88505.1"
                     /translation="MSVAFTKEGDSEAFAADLLDRPIS"
     assembly_gap    2928..2937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2938..3326
                     /gene="greA"
                     /locus_tag="PLH31_04420"
     CDS             <2938..3326
                     /gene="greA"
                     /locus_tag="PLH31_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQR88506.1"
                     /translation="AQGLAQLEAWLAEARAAYSAAQATGGVAEDRTAMARATRDLRYY
                     AARRASAQLTEPAGPTNVVHFGQAVTFDREDGRRQTFRIVGEDEADPAAGSVSYVSPL
                     ARALLGKTIGDTAMVAGGEVEIVSID"
     gene            3340..3924
                     /locus_tag="PLH31_04425"
     CDS             3340..3924
                     /locus_tag="PLH31_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQR88507.1"
                     /translation="MQAAIAAIEAVTPEFQWRIRDRGFAGLLKLMVEQQVSLASAAAI
                     WARVEAGLGEVSPAAVLALESADLQAFGLSRPKVRYARAIAQAHVSGLCDFDALRHKG
                     DDEAAAALMAITGIGRWTAETYLMFCEGRLDVFPGGDVALQEAMRWVDGAEVRPSEKQ
                     AYARAQAWKPYRAVAAHLLWRCYGAVKKGEIPPF"
BASE COUNT          629 a         1305 c         1360 g          724 t
ORIGIN      
        1 caagatgtcg ttcgagatgg agccgcgcgc ggcgggttat ccaaccacgg tagtctacga
       61 caagcagggc cgcgagcgag cccgtctggc gggagacgcc gactggtcca gccccgaggc
      121 gcgaggcctg gtcgagaagc tgctggccga gtgacggttg gtttcgacat cgatcccgcc
      181 gttctcgaac ccatagccga ccgggcctgg ccggcgcgag aacgctcggc gctgggtggg
      241 tggcggctga acgccaccgc cggctggtcc atgcgcagca atgcctgctg gccgctggct
      301 gagccggacc gtgacgtcga gacagccatc gaggccgccg aggcctggtt cgtctcccgg
      361 gggctgccgc gacggttcaa gttgacggag ggggcgacgg cgccggccga tctgtcccaa
      421 cgcctctcac ggcggggcta tgccgactgc aagaagaccc tggtgatggt cggccctgcg
      481 ctgggccgcg ccgatccggc tgtcgccttc tccgctgcgc ccgacgcgag cttcgaggcg
      541 gtgttcacgg cctcggcggg cggcaatgtc gaggacgcgc gcgagcggct ggaaaccctg
      601 gctcgcatcc cggctccggc gcggttcgcc cgtctggata tcgatagtcg ccctgccgcg
      661 ttgggtgctt gcgccgtcgg gggcgagtgg gtgggcctgt tcggcatgcg cactgcggtt
      721 gagcatcgcc gcaagggtct ggctcggctg gtgctcagag gcttgttggc cgaggcggcg
      781 tcgctcggcg cggttcacgc ctatctgcag gtcgaggccg acaacgcgcc ggccatcgcc
      841 ctgtatgagg ttgaagggtt ccgaacggcc tacacctatc gctactgggt tcgctagagg
      901 cccatctctg atcgcaaggc tgctggcgtg acgcccgccg cccgcagttc gcgcaaggtc
      961 tgggccttgt cgcgcttggc gtagcgcttc ccgtccgggc cggtcagcag acgatgatgg
     1021 cgataggttg gcgttggcag gcccagtagc gcctgcagca ggcgctggat atgcgccgct
     1081 tcgaacaggt cctcgccccg gatcacgtgc gtgatatcct gcagggcgtc atcgagaacc
     1141 acagcgaggt gataggcgac gccgacgtcc ttgcgagcca ggacgatgtc gccggcggta
     1201 tcaggacgcg cgcagatcag tccggtctgc ccctcggggc cggcgccctc ctcgatgaag
     1261 gtcagggctt cgaagccgcc agtcgccgcc ttcgccgccg tgagggagag gcgccacgca
     1321 aaggcctcgc cgcgcgccaa ccgctgggct tcttcaacgg cgggnnnnnn nnnnaagggg
     1381 ccgcccgtga agggcgcctg cggctcgtgc ggcgcacggc caatatccag ctcccggcgc
     1441 gtgcgaaaac agcgatagac gagaccgcgc ccctggaggc tttcgatggc ggcgtggtag
     1501 tcggccagat gctcggactg ccggcggaca ggtcgcgccc actccagccc aagccaggcc
     1561 aggtcttcca ggatcgcagt ctcgtactcc ggccgacagc gggtggcgtc gatatcctcg
     1621 atccgcagga caaagcgacc gttcatagcg cgcgcggcct cgaaggctgt cagggcggag
     1681 aaagcgtgac cgcgatgcag caggcccgtt ggcgagggcg caaaccgcgt ggcgaagccc
     1741 aaggacctag ccccggtcgt actttgcgaa catgccgagg tgctcgaata cggcgcgcag
     1801 gaaggcgtcc tcgccgccta gcagctcctt cagcggatcg aactgcttga agctgatcat
     1861 ttcggccaat ccatgccctg cgcaccaggc cgcgatggtg gccagttcca gatcaatgtc
     1921 gctgacgccg tcaagcgact gctcggagat ggtgtcgcgc acctggcagt aggcgccttc
     1981 ttctttcatc tgcnnnnnnn nnncggggag ggcgtccttg tcgcgagagc agtcatacat
     2041 cacgcggtag agggccggat tgtcccgcgc gaactgtacg taggtcacgc cgatgctggt
     2101 catccgctcg cgagcatccc tggctgaggc gcgcgattgg ctcagggcgg tatccagcgc
     2161 catccagcct tcgtgagcga ccgcctccag caattggccc ttgtccttga agtggtgata
     2221 gggcgcggcg gggctgaccc cggcctcgcg cgctacggcg cgcagtgaca gcgcggccgg
     2281 accctccctc tcaagaatgc ggcgcgcggc gtcgaccagc gcgcggctta gatcgccatg
     2341 gtgatacggg cgggattcgg tggctgggat ggtcatcggc ctcactctag cgcctccttt
     2401 ctaaaatttc atccaaacgc tgtaaagatc atcttgacgt cgtttagatc catgttatct
     2461 aagcaccgtc cagattggac caagcctttg acctgcaacc ggcgaggctc attcacagag
     2521 agggatttcg accatgctcc gctcgctcac cgcctttgaa gccgcctttg accgtatggc
     2581 cggcggctat tttctcgtgc tcgccctggc tgtcgccggc gctgtcgcgg gcgtcgtgct
     2641 gtaagatggc tttgtccgcg agcggcgggg ccggttctgc aatgaccgtc ccgctgccgc
     2701 gttcgtcgtc cgctgactgc ccgcccgcgc ttgaacccga tcgcgctggc ggctagcacg
     2761 aagcgccgac gcgcctggat ttccaacttc gcgccgggaa cgtcccgccg gcccccggat
     2821 ttccagatca tgtcgatttc aaacgtgttt ttccaatgag cgtcgccttc accaaggaag
     2881 gcgacagcga ggcgttcgcc gccgacctgc tcgatcggcc aatttccnnn nnnnnnncgg
     2941 cccagggtct cgcccaactc gaagcgtggc tcgccgaggc gcgcgccgcc tatagcgccg
     3001 ctcaggccac gggcggcgtc gccgaagacc gcactgccat ggcccgcgcc acgcgcgacc
     3061 tgcgctacta cgcagcgcgc cgggccagcg cacaactgac cgaacccgcc ggacccacaa
     3121 acgtcgtaca tttcggccaa gccgtaacct ttgatcgcga ggatggccgt cgtcagacct
     3181 tccgcatcgt cggcgaggac gaggccgatc ccgccgcggg ttcggtgtcc tatgtctcgc
     3241 ctttggccag ggcgctgctg ggaaagacca tcggtgacac ggccatggtc gccggcggcg
     3301 aggtcgagat cgtctcgatc gactagcgca agcatcgggt tgcaggcggc catcgccgcc
     3361 atcgaggccg tgacgccgga gtttcaatgg cgcatccgag accgaggctt cgctggactg
     3421 ctcaagctga tggtcgaaca gcaggtctct ctggcctcgg ccgccgccat ctgggcgcga
     3481 gtggaggcgg ggttgggcga ggtctcgccc gccgctgtgc tggcgcttga gagcgctgat
     3541 ctacaggcct ttggcctgtc gaggcccaag gtgcggtacg cccgcgccat tgcccaagcg
     3601 catgtttccg gtctctgcga cttcgacgcc ttgcgacata agggtgatga tgaggccgcc
     3661 gcggccctga tggcgatcac cggcataggg cgctggacgg ccgagaccta tctgatgttc
     3721 tgcgagggtc gtctggacgt ctttccgggt ggtgatgtgg cgcttcagga agccatgcgc
     3781 tgggttgacg gcgccgaagt ccgtccttcc gagaagcagg cctatgcgcg agcgcaggcc
     3841 tggaagccct atcgcgccgt tgcagctcac ctgctctggc gctgctatgg cgcggtcaag
     3901 aagggcgaaa ttccaccatt ctgatcactt gcggacagat gtcttggttc cgtcgcggag
     3961 ttggatcatc tttgggatgc gttcgggaat gaatggcgat tccctttgaa tgcgtaggaa
     4021 ggaggcccgt cttcagtccg atcgcgta
//
LOCUS       DAOMEO010000142         4047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2640_length_4047_cov_2.369990, whole genome shotgun sequence.
ACCESSION   DAOMEO010000142 DAOMEO010000000
VERSION     DAOMEO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_4047_cov_2.369990"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLH31_04430"
     CDS             complement(<1..471)
                     /locus_tag="PLH31_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQR88508.1"
                     /translation="MDDTLAQLPHAFAGKTVLVLGDVMLDRFIYGAVDRISPEAPVPV
                     IAVERETAMLGGAGNVARNVAALGGKAVLIGLIGDDDAGRALTAMLGDEAGVEAHLVT
                     DTARRTTEKVRYISGSHQMLRVDREDRGPAEAAALLSAFKQRLAAADVVVLSDYA"
     gene            complement(509..1438)
                     /locus_tag="PLH31_04435"
     CDS             complement(509..1438)
                     /locus_tag="PLH31_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQR88509.1"
                     /translation="MTLSALSPETQALTRRVTALSVAVAAVLIVVKTAAWWASGSVAM
                     LASLSDSGLDLVASLISFFAVRYAAAPPDAEHRFGHGKAEAFSSLMQGGLVFASGALI
                     GQEAIRAMFNPRPVEQGLLAMAVMVVSIVLTLGLIIAQSKVLKSTGSIAVSGDRAHYA
                     ADLGSNAVALVGIGAAAFLGVGWLDAFAGLIVALWLIWGAIGVFREAASQLMDRELPD
                     EDRARIVALATEDPRLLGVHQLRTRASGPYVHMQMHADLDAGISLAEAHSIMVAAENR
                     LLKAFPAADIIIHPDPRGQAESHGGLFAEAQHG"
     gene            complement(1505..>2103)
                     /locus_tag="PLH31_04440"
     CDS             complement(1505..>2103)
                     /locus_tag="PLH31_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQR88510.1"
                     /translation="LVTVCDHGLNASTFAARVIASTRAGLTSAVLGGLSALKGPLHGG
                     APGPVIDMLDAIGAPENARPWLEKALARGDRLMGFGHRIYRVRDPRADALKAAVRALS
                     GASKALPGRVAFAEAVEQAALQILRERKPDRPLDTNVEFYTALLLEALGLPPASFTGV
                     FAMGRTAGWIAHAREQLAGGKLIRPQSVYVGPGVRQAA"
     assembly_gap    2104..2113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2114..2581)
                     /locus_tag="PLH31_04445"
     CDS             complement(<2114..2581)
                     /locus_tag="PLH31_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQR88511.1"
                     /translation="MSQGLAHGLEGVIAAHTVLSDVDGAHGRLVIRGYAVEDLAGHTP
                     FEQAAHLLFDGFFDDMPADLAPALGAARVKVFAEVAALDEALAKRDPVEAMRALLARL
                     PDGDDLSTALLLLAAPAVFTPAVLRVAAGQTPVAPDPALSHAADILQMTRGQPA"
     gene            2657..3817
                     /locus_tag="PLH31_04450"
     CDS             2657..3817
                     /locus_tag="PLH31_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HQR88512.1"
                     /translation="MASDWLDAEQVLERLGIRPQTLYAYVSRGRIEAAAHPDDPRRSL
                     YRASDVAALHARKARGRRAADVAAEAIAWGEPVLASAITTVAGGRLWYRGRDAAVLAV
                     DHTLEQTARLLRGGHGAALKAARGRDVVAGETARARLFNALALRAGAEAPARGRAPLA
                     LAMEAASLLDAVVDAAAGEVTEGPAHQRLARAWGVDAAGADLLRRALVLLADHELNAS
                     TFAARVAASTGASLSAAALAGLCALSGPLHGGMAARVEAFVAEADRTGPARAVSARLA
                     QGSSTPGFGHPLYPDGDPRARALLAAFEPPALLADLRRETEAATGQPPNIDFALAGLA
                     RALALPADAPFILFAVARTAGWLAHAIEQGQTGRLIRPRARYVGEALVAGAG"
     gene            complement(3842..>4047)
                     /locus_tag="PLH31_04455"
     CDS             complement(3842..>4047)
                     /locus_tag="PLH31_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88513.1"
                     /translation="VNWQFVRNYDLAELLSVLREQVSSDDDVLTQFYDPGEVYPLLDG
                     ARDFADVLAIWSKLERGPNEEQP"
BASE COUNT          619 a         1346 c         1500 g          572 t
ORIGIN      
        1 ggcgtagtcg gacagcacca ccacgtcggc ggcggccaga cgctgtttga aggccgacag
       61 cagggcggcg gcctcggcag gcccgcgatc ttcccggtcg acgcgcagca tctggtgtga
      121 gccggagatg tagcggacct tctcggtggt ccggcgtgcg gtgtcggtga caaggtgagc
      181 ctcgaccccg gcctcgtcgc ccagcatggc ggtgagagcc cggccggcgt catcgtcgcc
      241 gatcagaccg atcagaacag ccttgccgcc cagggccgcg acattgcggg ccacattgcc
      301 ggccccgccc agcatcgcgg tttcacgttc gacggcgatc accggcacgg gggcttcagg
      361 cgagatgcgg tcgacggcgc catagatgaa gcggtccagc atcacgtcgc ccagcaccag
      421 cacggtcttg ccagcgaagg cgtggggcag ttgggccagg gtgtcgtcca tgaaggggcg
      481 gctcgtctcg aagcgggtct ggccgttact agccgtgctg ggcctcggcg aacagcccgc
      541 catggctttc ggcctgaccg cgggggtcag ggtggatgat gatgtcggcg gcgggaaaag
      601 ccttcagcag ccggttttcg gcggccacca tgatgctgtg cgcctcggcc agcgagatgc
      661 cggcgtccag gtcggcgtgc atctgcatgt gcacataggg ccccgacgcc cgggtgcgca
      721 gctggtgcac gcccagaagg cgcggatcct cggtggcgag ggcgacgatg cgggcgcggt
      781 cctcgtcggg cagttctcgg tccatcaact ggctggcggc ctcgcgaaaa acgccgatgg
      841 cgccccagat cagccacagg gccacgatca ggccggcgaa cgcgtccagc cagccgaccc
      901 ccaggaaggc ggccgcgccg atcccgacca gggccacggc gttggagccc agatcggcgg
      961 cgtagtgggc cctgtcgccc gagacggcga tggagccggt cgatttcagc accttcgact
     1021 gggcgatgat caggcccagg gtcaggacga tcgagaccac catcaccgcc atggccagca
     1081 ggccctgctc gaccggacgc ggattgaaca tggcccgaat ggcttcctgg ccgatcaggg
     1141 cgccggaggc gaagaccagg ccgccctgca tcaggctgga aaaggcttcc gccttgccgt
     1201 gaccgaagcg gtgctcggcg tccggcggcg cggccgcgta gcgcaccgcg aagaagctga
     1261 tcagcgaggc gaccagatcg agcccagagt ccgacagcga ggccagcatc gccaccgagc
     1321 cgctggccca ccaggcggcg gtcttaacca cgatcagcac cgctgccacc gccaccgaca
     1381 gggctgtgac gcgacgggtc agggcctggg tttcgggcga aagggcggat agggtcatgc
     1441 cgcttcctta acaagcggcg cggtctcacc ccagcgagaa cgactcgcgc gagtcgttct
     1501 cgcgtcaggc ggcctgtcgc acgccgggcc ccacatagac tgactgcggc cggatcagct
     1561 tgccgcccgc cagctgttcg cgcgcgtggg cgatccagcc ggcggtgcgg cccatggcga
     1621 acacgccggt gaagctggcc gggggcaggc ccagggcctc cagcagcagg gcggtgtaga
     1681 actcgacatt ggtgtccagc ggtcggtcgg gcttgcgctc gcgcaggatc tgcagcgccg
     1741 cctgctcgac cgcctcggcg aaggccacgc ggccgggcag ggccttcgac gcgcccgaca
     1801 gcgcgcgcac ggcggctttc agggcgtcgg cgcgcgggtc gcggacgcga tagatgcggt
     1861 ggccaaagcc catcagccgg tcgccgcgcg ccagggcctt ttccagccac gggcgggcgt
     1921 tctccggcgc gccgatggcg tcgagcatgt cgatcactgg gccgggcgcg ccgccgtgca
     1981 aggggccttt cagggccgac agtccgccca gcaccgccga ggtcaggccc gcgcgggtcg
     2041 aggcgatcac ccgggccgcg aaggtcgagg cgttcaggcc atggtcgcag accgtgacca
     2101 gatnnnnnnn nnnggccggc tggccgcgtg tcatctgcag aatgtcggcg gcgtgcgaca
     2161 gggcggggtc cggcgcgacg ggggtctggc cggccgcgac acgcagcacg gcgggggtga
     2221 agacggccgg ggcggccagc agcagcaggg cggtggagag atcatcgccg tcgggcaggc
     2281 gggccagcag ggcgcgcatg gcttcgacag gatcgcgctt ggcgagggcc tcgtccagcg
     2341 ccgcgacctc ggcgaacacc ttgacccggg ccgcgcccag ggcgggcgcc agatcagccg
     2401 gcatgtcgtc gaaaaaaccg tcgaacagca ggtgcgccgc ctgctcgaac ggggtgtggc
     2461 cggccagatc ctcgacggcg tagccgcgaa tgaccagacg gccgtgggcg ccatcgacgt
     2521 cggacagaac ggtgtgggcg gcgatcacgc cttcaagccc gtgggcaaga ccttgcgaca
     2581 tttgaggtct cctttcgggc ggcaattcac gctcggcgcg cttgatcgtc aatcttgatc
     2641 aatataatca atctatatgg cgagcgactg gctggacgcg gaacaggtgc tggagcggct
     2701 ggggatccgc ccccagaccc tctacgccta tgtcagccgg ggccggatcg aggccgccgc
     2761 ccatcccgat gatccccgac gcagcctgta tcgcgcctcc gacgtcgcgg ccctgcacgc
     2821 ccgcaaggcc cgggggcgtc gcgccgccga tgtggcggcc gaagccatcg cctggggcga
     2881 gccggtgctg gcttcggcga tcaccacggt ggcgggcggc aggctctggt accgggggcg
     2941 cgacgctgcg gtgctggctg tcgatcacac cctggaacag accgcccgcc tgctgcgggg
     3001 cggacatggc gcggcgctga aggcggcgcg ggggcgggat gtcgtggcgg gcgaaaccgc
     3061 gcgggctcga ctgttcaacg ctctggcgct gcgagcgggg gcggaggccc cggcgcgcgg
     3121 gcgggcgccg ctggccctgg ccatggaagc ggccagcctg ctcgacgccg tggtcgacgc
     3181 ggcggcgggc gaggtcaccg aaggtccggc gcaccagcgc ctggcgcggg cctggggtgt
     3241 cgatgcggcg ggggccgact tgctgcggag ggcgcttgtg ctgctggccg accacgaact
     3301 gaacgcctca accttcgcgg cccgggtcgc ggcctcgacc ggggcgtcgc tgtcggccgc
     3361 cgccttggcc gggctctgcg ccctgtcggg gccgctgcac ggcggcatgg ccgcgcgggt
     3421 cgaggccttt gtcgctgagg ccgaccggac gggccccgcc agggccgtgt cggcgcgtct
     3481 ggcgcaggga tcatcgacgc cgggctttgg acatccgcta tatccggacg gggatcctcg
     3541 ggcgcgggcc ttgctggcgg cgttcgaacc gccggccctg ctggcggacc tgcggcggga
     3601 gacggaggcc gcgacggggc agccgcccaa tatcgatttc gccctggcgg gcctggcccg
     3661 ggcgctggcc ctgccggccg atgcgccgtt catcctgttc gcggtggcgc gcacggcggg
     3721 atggctggcc catgcgatcg agcaggggca gaccggacgg ctgatccggc cccgggcgcg
     3781 gtatgtgggc gaggcgctgg tggcgggggc tggctgagga tctcccgatg cgggcggacg
     3841 actaaggctg ttcctcgtta gggcctcgct caagcttgga ccatatcgcc aggacatcag
     3901 cgaaatctcg tgctccatca agtaacggat agacctcacc aggatcataa aactgagtca
     3961 gaacatcatc atccgatgaa acctgttctc ttaatacgga taaaagctca gctaagtcat
     4021 aatttctaac gaattgccaa ttcactg
//
LOCUS       DAOMEO010000143         4036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2646_length_4036_cov_2.397890, whole genome shotgun sequence.
ACCESSION   DAOMEO010000143 DAOMEO010000000
VERSION     DAOMEO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2646_length_4036_cov_2.397890"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            534..1442
                     /locus_tag="PLH31_04460"
     CDS             534..1442
                     /locus_tag="PLH31_04460"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HQR88514.1"
                     /translation="MVKQPLTDDPQEGSGGGPGEDRPAPRFATYFHIHLVSDSTGETL
                     NAMARAVCARFTDILPIEHIYALVRSTRQLDRALQEIAGAPGVVMHTIIDPGLRTALE
                     EGCRRLEMPCIAALDPVVSAMSRYLGARISTRVGAQHALNNDYFDRIEALDYAIAHDD
                     GQGGQDLTQADVILVGVSRTSKTPTCIYLAHRGVRAANVPLVPGRPPPSELFELKNTL
                     IVGLMTSPDRLIQIRRNRLLSLKENRESDYIDTDAVRSEIIAARRLFEKMGWPIIDVT
                     RRSVEETAAAIINLLSGGRGQVEVLG"
     gene            1439..2038
                     /locus_tag="PLH31_04465"
     CDS             1439..2038
                     /locus_tag="PLH31_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQR88515.1"
                     /translation="MSRTPVTLASKSAARQAILRNAGIDFEAVVAGVDEDATKAGLLA
                     EGASPREVADALAELKAVKVSGKRPGLVIGADQTLDFAGRLIDKVETLDQARALLLEL
                     RGQTHKLHSAVVVARDGKPIWRVVQSAKLSVRPFSDAWLDGYLQRRGEHILHCVGCYE
                     LESEGVQLFDAIDGDYFTILGLPLVGLLDFLRLHGALEA"
     gene            2047..2904
                     /gene="aroE"
                     /locus_tag="PLH31_04470"
     CDS             2047..2904
                     /gene="aroE"
                     /locus_tag="PLH31_04470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQR88516.1"
                     /translation="MTGGLTGAAIVAGVCGAPIKHSMSPVIHNAWIQAAGLDAAYVPF
                     APAEDRFEAFIEGLRGGAIRGLNVTIPFKERALAVADTASDLARMAGAANLLIFEADG
                     TIRADNTDGPGLLGAIAAQAPGFDVSAAPVIILGAGGAARGAVAALLLAGAPQVRIVN
                     RTLARAGELATTFGAKVIAADEAALPGLLTDAGLVINATSLGLGGGDGPAASLELTPA
                     DAVIVDMVYKPLRTEFLQRAQALGRRTVDGLEMLLRQAVPSFEAFYGQTPPTTVDVRR
                     LALHLLGEA"
     gene            2908..3531
                     /gene="coaE"
                     /locus_tag="PLH31_04475"
     CDS             2908..3531
                     /gene="coaE"
                     /locus_tag="PLH31_04475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674871.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQR88517.1"
                     /translation="MIIVGLTGSIGMGKSTTSAMFEAEGVPVYDSDAAVHALYAAGGA
                     AVAPVEAAFPGVVVDGAIDRTRLSAAVMGDPEALQRLEAIVHPLVGANRIGFFEKAAA
                     DGKDIVVLDVPLLYETGGDKRVHKVVVVSAPLEVQRQRVLARPGMDEAKFEAILARQL
                     PDAEKRARADFVIDTSQGLDHARQQVRAVLEALRQPERLDGGSEPAH"
     gene            3538..>4036
                     /gene="dnaQ"
                     /locus_tag="PLH31_04480"
     CDS             3538..>4036
                     /gene="dnaQ"
                     /locus_tag="PLH31_04480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQR88518.1"
                     /translation="MAREIILDTETTGFDPKTGDRLVEIGCIEVVDFMPTGRSFHEYC
                     DPLRDMPAEAQKVHGLSSEFLTGKPKFHEIADRFIEFVGDSPLIAHNAAFDRSFVNFE
                     LEKCGRAALPEPRWVDTLAMAKKRFPGMYNSLDALCKRFKISLDSRDKHGALIDAHLL
                     AEVYLE"
BASE COUNT          658 a         1329 c         1355 g          694 t
ORIGIN      
        1 agcctggcgc atgaaccaga ccggcggacg ctcggcggtt tgtccggcca ggacggagag
       61 cagcttgggc gtctgggggg tcaggctggg tgcgttcatg aggggcgtta aatcccgtcc
      121 tgcccttggg ctcaagcccg aaggccaatc ttcatttctt aataagatct taagtttgga
      181 agcaggagag gctgggcaag tgggaactgg ggaaaaccct gcggcgcgga ctgactccca
      241 catcccgtcc agccttgtac ccacgatccc ccacatgggg atggagccct gttaatcatg
      301 gggatggatg gctagagcct ttagttcctt aacgaactct taacgagctt ccgtgtgctt
      361 tacgtcccgt taacctttct tccccgagca tgggacggca ttgatccaag atccagggat
      421 gcgggctcgg cttggggatg ttacgcggtc aatgcggccg ttgatcacag cggcgacatg
      481 acacgacgct tcgggacgac tttggcgccc acgcgtcccc agaggcaggc caagtggtta
      541 agcaaccgtt aacggatgat ccacaggaag gttcgggcgg cggaccgggc gaagaccgcc
      601 cggcgccgcg tttcgcgacc tattttcata tccatctggt gtcggactcg actggcgaga
      661 cgctgaacgc gatggcccgg gctgtctgcg cccggttcac ggacatcctg ccgatcgagc
      721 acatctatgc cctggtccgc tccacccggc agctggaccg ggcgctgcag gagatcgccg
      781 gcgcgcccgg cgtggtgatg cacaccatca tcgatccggg gctgcgcacg gcgctggagg
      841 agggctgtcg acgtctggag atgccctgca ttgcagcgct ggatcctgtg gtcagcgcca
      901 tgtcgcgcta tctgggcgcc aggatctcta cgcgggtggg tgcgcagcac gcgctgaaca
      961 acgactattt cgaccgcatc gaggcgctgg attacgccat cgcccatgat gacggccagg
     1021 gcggccagga cctgacccag gccgacgtga tcctggtggg cgtatcgcgc acctccaaga
     1081 cgcccacctg catctatctg gcccatcgcg gggttcgggc cgccaacgtg ccgctggtgc
     1141 cgggccggcc gccgccgtcc gagctgttcg agctgaagaa caccctgatt gtcggcctga
     1201 tgacctcgcc cgaccggctg atccagatcc gccgcaaccg tctgctctcc ctgaaggaga
     1261 accgcgagag cgactatatc gacacggacg cggtgcgcag cgagatcatc gccgcccgcc
     1321 gcctgttcga aaagatgggc tggccgatca tcgacgtcac ccgccgctcg gtggaagaga
     1381 cggccgcggc gatcatcaac ctgttgtcag gcggtcgcgg ccaggtcgag gtgctgggat
     1441 gagccgcacg ccggtcactc tggcgtcgaa gagcgcagcc cgacaggcga tcctgcgcaa
     1501 tgccgggatc gacttcgagg cggttgtcgc cggtgtggat gaagacgcga ccaaggcagg
     1561 ccttctggcc gagggcgcct cgccccgcga ggtcgccgac gccctggccg agctgaaagc
     1621 ggtcaaggtc tcgggcaagc ggcctggcct ggtgatcggc gctgaccaga ccctggattt
     1681 cgccggtcgg ctgatcgaca aggtcgagac cctggaccag gcccgcgccc tgctgctgga
     1741 gctgcgcggc cagacccaca agctgcactc ggccgtggtg gtcgctcgcg acggcaagcc
     1801 gatctggcgg gtggtgcaga gcgccaagct gtcggtgcga cccttcagcg acgcgtggct
     1861 ggacggctat ctgcagcgcc gcggtgagca catcctgcac tgcgtgggct gctacgagct
     1921 ggagagcgag ggcgtccagc tgttcgacgc catcgacggc gactatttca ccatcctggg
     1981 cctgccgctg gtggggcttc tggacttcct gcgtcttcat ggagccctgg aggcatgagt
     2041 tcccaaatga cggggggcct caccggcgcg gcgatcgtcg ccggcgtctg cggcgcgccg
     2101 atcaagcact cgatgagccc ggtgatccat aatgcctgga tccaagcggc cggcctcgac
     2161 gccgcctatg tgcccttcgc gccggccgaa gaccgcttcg aggccttcat cgagggcctg
     2221 cgcggcggcg ccatcagggg tctgaacgtc accatcccct tcaaggagcg cgccctggcg
     2281 gtcgccgaca ccgccagcga cctggcgcgc atggccggcg ccgccaatct gctgatcttc
     2341 gaggctgacg gcacgatccg cgccgacaac accgacggtc ctggcctgct gggcgccatt
     2401 gccgctcagg cgcccggctt tgacgtttcc gccgcgccgg tgattatcct gggcgcgggc
     2461 ggcgcggcgc gcggggcggt ggcggccctg ctgctggccg gcgcgcccca ggttcggatc
     2521 gtcaatcgca ccctggcccg ggccggggaa ttggcgacga cgttcggcgc caaggtgatc
     2581 gccgccgatg aagccgccct gcccggcctg ctcaccgacg cgggtctggt gatcaacgcg
     2641 acatccctgg gcctcggcgg cggcgacggc ccggccgcgt cgctggagct cacacccgcc
     2701 gatgcggtga ttgtggacat ggtctacaag cctctgcgca ccgaatttct tcagcgcgcc
     2761 caggccctgg gtcgtcgcac ggtcgatggc ctggaaatgc tgctgcgcca ggccgttccg
     2821 tcgttcgagg ccttttatgg ccagaccccg ccgaccacgg tcgatgtgag gcgtctggcg
     2881 ctccacctgc tgggcgaggc ctgaatcatg atcatcgtcg gcctcaccgg ctccatcggc
     2941 atgggcaagt cgaccacctc ggcgatgttc gaggccgagg gcgttcccgt ctatgacagc
     3001 gacgcggcgg tgcatgcgct ctatgcggcc ggcggggcgg ccgtggcccc ggtcgaggcg
     3061 gcctttcccg gcgtcgtcgt tgacggggcc atagaccgca cccggctgag cgcggccgtg
     3121 atgggcgatc cggaagcctt gcagaggctg gaggccatcg tccatccgct ggtcggcgcc
     3181 aaccggatcg gcttcttcga aaaggcggcg gccgacggca aggacatcgt cgtgctggac
     3241 gtgccgctgc tgtatgagac cggcggggac aagcgggtcc acaaggtggt tgtggtctcg
     3301 gccccgcttg aggttcagcg acagcgggtt ctggctaggc ccggcatgga cgaggccaag
     3361 ttcgaggcca ttctggcccg ccagctgccc gacgccgaga agcgggcgcg ggccgatttc
     3421 gtgatcgaca ccagccaggg gctagaccac gcccgtcaac aggttcgtgc ggtgcttgag
     3481 gccctgcgac aacctgagcg tcttgatggc gggtccgaac cggcccattg aatcggcatg
     3541 gcccgggaaa tcatcctcga caccgaaacc accggcttcg atccgaagac gggcgaccgt
     3601 ctggtcgaaa tcggctgtat cgaggtggtc gacttcatgc ctacgggccg gtcgttccat
     3661 gaatattgcg atcccctgcg cgacatgccg gccgaagcgc agaaggttca cggcctgtcg
     3721 tcggaattcc tgaccggcaa gcccaagttc cacgagatcg ccgaccgctt catcgagttt
     3781 gtcggcgaca gcccgctgat cgcccacaac gccgccttcg accgcagctt cgtcaatttc
     3841 gagctcgaaa agtgcggccg cgccgccctg ccggaaccgc gctgggtcga taccctggcc
     3901 atggccaaga agcgcttccc gggcatgtac aactcgctgg acgccctgtg taagcgcttc
     3961 aagatcagcc tcgacagccg agacaagcac ggcgccctga tcgacgccca cctgctggcc
     4021 gaggtctatc tggaat
//
LOCUS       DAOMEO010000144         4034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2649_length_4034_cov_3.123900, whole genome shotgun sequence.
ACCESSION   DAOMEO010000144 DAOMEO010000000
VERSION     DAOMEO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2649_length_4034_cov_3.123900"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2501
                     /locus_tag="PLH31_04485"
     CDS             <1..2501
                     /locus_tag="PLH31_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQR88519.1"
                     /translation="VLELGEAEYMVRASGYLKTLDDFRAIPLRAGAGGVPITLGEIAT
                     VQIGPEMRRGVSELNGQGEVAGGVVILRSGKNARETIAAVKIKLEELKRSLPAGVEVV
                     TTYDRSQLIDRAIENLTGKLGEEFLVVAIVCALFLGHVRSALVAIVTLPLGVLIAFLV
                     MRMQGVNANIMSLGGIAIAIGAMVDAAVVMIENAHKHLERWAHDHPGETLTPERRWTL
                     VTEAAAEVGPALFLSLVIITLSFVPVFALQAQEGRLFAPLAFTKSYAMAGAALLSVTL
                     VPVLMGYLIRGRIPSENANPLNRALTRIYRPALDAVMRRPKATLVIALLVFATTAWPL
                     SRLGGEFIPHMDEGDLLYMPSALPGISAAKASELLQQTDRLIMTVPEVKTVFGKAGRA
                     ETATDPAPMEMFETTIQFKPRDQWRPGMTPDKLVETLDRAVQVPGLANVWVPPIRNRI
                     DMLATGIKSPIGVKVSGTNLQDLDRVAAAIEAVAKKTPGVSSALAERLTGGRYVDVDI
                     DRTAAGRFGLNIADVQAIVSGAIGGETVGETVEGVARYPISVRYPRELRDSLEGLRAL
                     PILTPAGQQITLGSVAKVQIVDGPPMLKTENARPSTWVYVDVRGRDLSAVVGDLQRAV
                     AKSVPLPPGVSVAYSGQFEYLQRAAERLKVVVPATLLIIFVLLYITFGRFDEAALIMA
                     TLPFALTGGIWTLYILGYHQSVATGVGFIALAGVSAEFGVVMLIYLKHALADRGPNPS
                     QGEVEAAVREGALLRVRPKAMTVAVILAGLAPILVGHGAGSEVMSRIAAPMIGGMLTA
                     PLLSMLVIPAGYLLLRRRWPASATTSASTQDGHA"
     gene            complement(2586..3860)
                     /locus_tag="PLH31_04490"
     CDS             complement(2586..3860)
                     /locus_tag="PLH31_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88520.1"
                     /translation="MSADDPEIEDRDAVLNRECFCVTLDSAGLLTAIQAEASNPDLAA
                     ALLSTRPHLFASMPVFIAEADLKAMLDVVAAVEAAAADPAYQAAALAWAPAIAQHDFG
                     PRGVFMGYDFHLGDGGPKLIEVNTNAGGAFLNAFLAQAQGACCREAADVARAAPAGEF
                     EAAVWAMFVGEWRLQGRSGQPETVAIVDDRPQEQYLFPEFLLVQRFFERQGLRAWIVD
                     PRDLRFADGRLRHNGEPVDLVYNRLVDFSLGAAGHEALREAYTAGAVVLTPNPRNHAL
                     FADKRNLTLLSDPAAAPGWKTTPGQQHSLAAVPRTRMVTAENADDLWSARKRYFFKPA
                     GGHGGKAVYRGDKMTRGVWAEVLQGGYIAQELAIPTERRIRIDGEVKVLKLDVRLYTY
                     RGLPLLTAARVYQGQTTNFRTPGGGFAPVFVT"
     gene            complement(3908..>4034)
                     /locus_tag="PLH31_04495"
     CDS             complement(3908..>4034)
                     /locus_tag="PLH31_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HQR88521.1"
                     /translation="EFAPYIGVSWDRKAGKTADYARARGEDIEATSVVVGIKAFF"
BASE COUNT          631 a         1368 c         1375 g          660 t
ORIGIN      
        1 cggtgctgga gctgggcgaa gccgaataca tggttcgcgc cagcggctat ctgaagacgc
       61 tcgatgactt ccgcgccatt ccgctgcgcg ccggcgccgg gggcgtgccg atcaccttgg
      121 gcgagatcgc caccgtacaa atcgggccgg agatgcggcg cggggtctcc gagctcaatg
      181 gccagggcga agtggccggc ggcgtggtca tcctgcgctc gggcaagaac gcgcgcgaga
      241 ccatcgccgc ggtgaagatc aagctggaag agttgaagcg cagcctgccc gccggcgtcg
      301 aggtagtcac gacctacgac cgctcgcagc tgatcgaccg cgccatcgag aacctgacgg
      361 gcaagctggg cgaagaattc ctggtggtgg cgatcgtctg cgcgctgttc ttggggcacg
      421 tccgctcggc tctggtcgcc atcgtgacct tgccgctggg cgtgctgatc gccttcctgg
      481 tgatgcggat gcagggggtc aacgccaaca tcatgtcgct cggcggcatc gccatcgcca
      541 tcggcgccat ggtcgacgcc gccgtggtga tgatcgagaa cgcccacaag cacctcgagc
      601 gctgggcgca cgatcatccc ggcgaaaccc tgaccccgga gcggcgatgg actctggtga
      661 ccgaggccgc tgccgaggtc ggtccggcgc tgttcctcag cctggtgatc atcaccctgt
      721 cgttcgtccc ggtgttcgcc ctgcaggcgc aggagggccg gctgttcgcg ccgctggcct
      781 tcaccaagag ctacgccatg gcgggcgccg ccctgctctc ggtcacgctg gtgccggttt
      841 tgatgggcta cctgatccgc gggcgcattc cctcggaaaa cgccaatccc ctgaaccgcg
      901 ccctgacccg gatctatcgg ccggcgctcg acgcggtgat gcggcgaccg aaggcgacgc
      961 tggtgatcgc cctgctggta ttcgccacca cggcctggcc gctcagtcgg ctggggggtg
     1021 agttcatccc ccacatggac gagggcgacc tgctctacat gccgtccgcc ctgcccggca
     1081 tctccgccgc caaggcctcg gagctgttgc aacagaccga ccggctgatc atgaccgtgc
     1141 cggaggtgaa gacggtgttc ggtaaggccg gccgcgccga gacggccacc gaccccgcgc
     1201 cgatggagat gttcgagacc acgatccagt tcaaaccccg cgaccagtgg cggcccggca
     1261 tgacgccgga caagctggtc gagaccctcg accgggccgt gcaggtcccc ggcctggcca
     1321 atgtctgggt gccgcccatc cgcaaccgca tcgacatgct ggcgaccggc atcaagagcc
     1381 cgatcggggt caaggtgtcg ggcacgaacc tgcaggacct cgaccgggtg gcggcggcga
     1441 tcgaggccgt ggccaagaag acgcccggcg tgagctcggc cctggcggaa cgcctgaccg
     1501 gcgggcgcta cgtcgatgtc gacatcgacc gcacggcggc ggggcgcttc ggtctcaaca
     1561 tcgccgacgt ccaggcgatc gtatcaggcg cgataggcgg cgagacggtc ggcgagaccg
     1621 tcgagggcgt cgcgcgctac ccgatcagcg tgcgctatcc gcgcgagctg cgcgacagcc
     1681 tcgagggact gcgcgccttg ccgatcctca cgccggccgg ccagcagatc accctgggct
     1741 cggtcgccaa ggtgcagatc gttgatggcc cgccaatgct gaagaccgag aacgcccggc
     1801 cgtcgacgtg ggtctatgtc gatgtgaggg gccgcgacct cagcgccgtc gtcggcgacc
     1861 ttcagcgcgc cgtcgccaag tccgtacccc tgccgcctgg ggtgtcggtc gcctattccg
     1921 gacagttcga atatctgcag cgcgccgctg aacggctgaa ggtggtcgtg ccggccactc
     1981 tgctgatcat cttcgtcctg ctctacatca ccttcgggcg cttcgacgag gccgcgctga
     2041 tcatggccac cctgcccttc gctctcaccg gcggcatctg gaccctgtac attctgggct
     2101 accaccagtc ggtggcgacc ggggtcgggt tcattgccct ggcgggggtc tcggcggagt
     2161 tcggggtggt gatgctgatc tacctcaagc atgccctggc cgaccgtggt cccaatccgt
     2221 cgcaaggcga ggtggaggcc gcggtgcgcg aaggggcgtt gctgcgcgtg cggcccaagg
     2281 cgatgacggt cgcggtcatc ctggccggcc tagctccgat cctcgtcggc cacggcgccg
     2341 gctcggaggt gatgagccgg atcgccgccc cgatgatcgg cggcatgctg accgcgccgc
     2401 tgctgtcgat gctggttatc cccgccggtt acctgctgtt gcggcgccgg tggccggcat
     2461 cggcgacgac gtccgcctca acccaggacg gccacgcatg acgagcttcg gggtcggctt
     2521 agattgagga gcaggccccg cccgggccta tctcagcaag ggcccaaggc ggggaaggtc
     2581 tcaagtcagg tgacaaacac cggggcgaac ccgccgcccg gcgtccgaaa attggtcgtc
     2641 tggccttggt agacacgcgc ggccgtcagg agtggtaagc ccctgtaggt gtagaggcgc
     2701 acgtccagct tcagcacctt gacctccccg tcgatcctga tccggcgttc ggtcgggatg
     2761 gccagttctt gggcgatgta gccgccttgc agaacctccg cccacacgcc gcgggtcatc
     2821 ttgtcgccgc ggtagaccgc cttgccgcca tggcctccgg ccggtttgaa gaagtaccgc
     2881 ttgcgcgctg accacaggtc gtccgcgttt tcggcggtca ccatccgggt ccgggggacc
     2941 gccgcgaggc tatgctgttg accgggcgtc gtcttccagc cgggcgccgc ggccgggtcc
     3001 gacagcagcg tcaggttgcg cttgtcggcg aagagcgcgt gatttcgagg attgggcgtc
     3061 aagacgacgg ccccggccgt ataggcctcc cgtagagcct catggcctgc ggccccgagc
     3121 gagaaatcga cgaggcgatt gtagacgaga tcgacgggct cgccattgtg tcgcagccgg
     3181 ccatcggcaa accgcaggtc gcggggatcg acgatccagg cgcgcaggcc ctggcgttcg
     3241 aagaagcgtt ggaccagcag gaattccgga aagagatatt gctcctgcgg ccggtcatcg
     3301 acgatggcga ccgtctcggg ctggccagac cggccctgca accgccactc gccgacgaac
     3361 atcgcccaga ccgccgcctc gaactctccc gccggcgccg ctcgcgccac gtctgcggcc
     3421 tctcggcagc aggcgccctg ggcctgggcg aggaaggcgt tcaggaaagc gccaccggcg
     3481 ttcgtgttga cctcgatcag cttcgggcca ccgtcgccca gatggaaatc gtagcccatg
     3541 aagacgccgc gcggcccgaa gtcatgctgg gcgatggccg gcgcccacgc caacgccgct
     3601 gcctgatagg cgggatctgc cgccgccgcc tcgaccgccg cgacgacgtc gagcatggcc
     3661 ttgaggtccg cctcggcgat aaacaccggc atgctggcga acaggtgcgg tcgggtggac
     3721 agcaaggcgg cggcgaggtc agggttggac gcctccgcct ggatcgctgt caacaggccg
     3781 gcgctgtcca gcgttacgca aaagcactcc ctgttgagaa cggcgtcgcg atcctcgatc
     3841 tcggggtcgt ctgccgacat ggaggtgatc ccttcggact tactatgcgc gccgacgcac
     3901 gtcagaccta gaagaaggcc ttgatgccca ccacgacgct ggtcgcctca atgtcctcgc
     3961 ctcgcgcccg agcgtagtcg gcggttttgc cggccttgcg gtcccaggac acgccgatat
     4021 agggcgcgaa ctca
//
LOCUS       DAOMEO010000145         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2655_length_4024_cov_2.668682, whole genome shotgun sequence.
ACCESSION   DAOMEO010000145 DAOMEO010000000
VERSION     DAOMEO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_4024_cov_2.668682"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="PLH31_04500"
     CDS             complement(<1..1210)
                     /locus_tag="PLH31_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HQR88522.1"
                     /translation="MSLEPAFDAFAQAYDAGQPQVVWTRLIDDLETPVSAYLKIGQGK
                     PYAFLFESVEGGAWRGRYSIVAMKPDFVWRCRGDQAEVAEGDAIARGEFTPQAGGALD
                     SLRDLIAASRIELPKGLPPMAAGVFGALGYDMVRLAERLPDINEDTTGLPDGIMTRPS
                     IVAVFDAIAQEIILVTTVRPTAGVSAQDAHHAARARIETVMADLRRPLAHDAPRKGHA
                     PIEFTTPVSREDLDPFALYRSLRRTNPSPFLYFLNLDGCHLVGSSPEILVRLRDGKVT
                     IRPIAGTRPRGATPEEDLALEAELLADPKERAEHLMLLDLGRNDVGRVSMLKEPGKNT
                     PAQPSKGPRVKVTESFKVERYSHVMHIVSNVEGVAPEGVDPVDVLMAALPAGTLSGAP
                     KVRAMEIIDEL"
     gene            complement(1207..>2828)
                     /locus_tag="PLH31_04505"
     CDS             complement(1207..>2828)
                     /locus_tag="PLH31_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR88523.1"
                     /translation="VREQMSNLHPNQRPFDPITGKFDPKMYEALLRENGLTPDSYQAS
                     LSDEIAQTHVFTGVANGLHAPSIYAALQAAYGLESRDLAAFAINPATIERPTDPTDAQ
                     LTTFMKENADRLTRPETRVLSIMRVSAQALEPSVTINPADVQKTFDFRKDTLATPETR
                     SLVQILAPDAKAAAVIAQRLAKGDQPAVVASAYGKQPVIISDKPKSALPDRKVADAAF
                     SLAVGQVSPPITGNLGLSVVKVTKITPGAGPTLESVRPQIEAELRTQGAQAKAYDQTQ
                     TYQDAHDAGSDLVAAATKAGAMVMTTAPITAQGADQTGQPIPGLTPDILKTAFDLSAG
                     GESELVELGKGEYYAVKVEKIVPAALPALADVRPQLVQVWLQNEMTERLRARAEAMAA
                     RVKKGESMDAVAASAGSKVQRVPGISRENAQQHQGLGRDLLLATFNAKPGDVFASRAG
                     QMAFVVAKLETVRTGKIADMARITQAMRPQVDQGLMRDLGEAARLGAQARVKTKTNLT
                     LARQAIGVDTDALAKAEAAQNSAGKTGAAKK"
     assembly_gap    2829..2838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2839..3147)
                     /locus_tag="PLH31_04510"
     CDS             complement(<2839..3147)
                     /locus_tag="PLH31_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQR88524.1"
                     /translation="MLAGFRTFAKSPFAVILFGLLILSFAVFGISDVFNGPRMSGVVS
                     AGSRTVSATDFKTRYENYRKETERRSGEALTPDQAVERGVDRQILQEIALQESMAAVV
                     E"
     gene            3308..>3521
                     /locus_tag="PLH31_04515"
     CDS             3308..>3521
                     /locus_tag="PLH31_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQR88525.1"
                     /translation="MTLSSLTPRPLIAGNWKMNGLAESLDEAHAIVAGLDASPAAARV
                     AICPPATLLHRLSQALEGSSVLSGGQD"
     assembly_gap    3522..3531
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3532..>4024
                     /locus_tag="PLH31_04520"
     CDS             <3532..>4024
                     /locus_tag="PLH31_04520"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQR88526.1"
                     /translation="DIAASMLIDAGASLVILGYSERRVDHDETSALVAAKVEAALAAG
                     LEPIICVGESLEQREAGAALEVVTTQVRESLPGSLAGQAFSVAYEPIWAIGTGRTATV
                     ADIEDMHAAIRAQLVAMFGEAGKAPPILYGGSVKPDNAAEILRAAEVGGALVGGASLK
                     ASDS"
BASE COUNT          617 a         1286 c         1414 g          687 t
ORIGIN      
        1 ccagctcgtc gatgatctcc atggcccgca ccttgggcgc gcccgacagc gtgccggcgg
       61 gcagggcggc catcagcaca tcgaccggat ccacgccctc gggcgcgacg ccctcgacgt
      121 tggagacgat gtgcatcacg tggctgtagc gctcgacctt gaagctttcg gtcaccttga
      181 cgcgcgggcc cttggacggt tgagccggcg tgttcttgcc cggctccttc agcatcgaga
      241 cgcggcccac gtcgttgcgg cccaggtcga gcagcatcag gtgctcggcg cgttccttgg
      301 gatcagccag cagctcggct tccagcgcca gatcctcttc cggagttgcg ccgcgcggac
      361 gggtgccggc gatggggcgg atggtgacct tgccgtcgcg cagccgcacc agaatctcgg
      421 ggctggagcc caccagatgg cagccgtcca ggttcaggaa atacaggaac ggcgatggat
      481 tggtccggcg cagcgagcgg tacagcgcga acggatccag gtcctcgcgg ctgacaggcg
      541 tggtgaactc gatgggcgca tggcccttgc gcggcgcgtc gtgggccagg ggccggcgca
      601 gatcggccat caccgtctcg atgcgcgccc gtgcggcgtg atgggcgtcc tgcgccgaaa
      661 cgccagcggt cggacgcacc gtcgtgacca gaatgatctc ctgggcgatg gcgtcgaaca
      721 ccgccacgat cgacggccgg gtcatgatcc cgtccgggag gccggtggtg tcttcattga
      781 tgtcaggcag gcgctcggcc agccgcacca tgtcatagcc cagggcgccg aacacgccgg
      841 ctgccatggg cggcaggccc ttgggcagct cgatccgcga ggcggcgatc aggtcgcgca
      901 ggctgtccag cgcgccgccg gcctggggcg tgaactcgcc gcgagcgatg gcgtcgccct
      961 cggcgacctc agcttgatcg ccgcgacaac gccagacgaa gtccggcttc atcgccacga
     1021 tggaatagcg gccgcgccag gcgccgcctt cgacgctttc gaacaggaaa gcatagggct
     1081 tgccctggcc gatcttcaga taggccgaga ccggggtctc cagatcgtcg atcagccggg
     1141 tccagaccac ctggggctgg ccggcgtcat aggcctgggc gaaggcgtcg aaggcgggct
     1201 caaggctcat ttcttggccg caccggtctt gcccgcactg ttctgggccg cctcggcctt
     1261 ggccagggcg tcggtgtcga cgccgatcgc ctgacgggcg agggtcaggt tggtcttggt
     1321 cttcacgcga gcctgggccc ccaggcgagc ggcttcgcca aggtcacgca ttaggccctg
     1381 gtcgacctgc ggacgcatgg cctgggtgat ccgggccatg tcggcgatct tgcccgtgcg
     1441 aacggtttcc agcttggcga ccacgaaagc catctggccg gcccgtgagg cgaagacatc
     1501 acccggcttg gcgttgaacg tggccagcag caggtcgcgg ccaaggccct ggtgttgctg
     1561 ggcgttctcg cggctgatgc ccgggacgcg ctggaccttg gagccggccg acgcagcgac
     1621 agcgtccatc gactccccct tcttgacgcg agcggccata gcctcggccc tggcgcgcag
     1681 tcgttcggtc atctcgttct gaagccagac ctggaccagc tgcgggcgaa catccgccag
     1741 agcaggcaga gcggcgggaa cgatcttctc aaccttgacg gcgtaatatt cgcccttgcc
     1801 gagctcgacg agttcgcttt cgccgcccgc cgacaggtca aaggcggtct tgaggatgtc
     1861 cggggtcagg ccggggatcg gttgacccgt ctgatcggcg ccctgagccg tgatcggggc
     1921 tgtggtcatc accatggcgc cggccttggt ggcggcggcg accaggtctg agccagcgtc
     1981 gtgggcgtcc tgataggtct gggtctggtc ataggccttg gcctgcgcgc cctgggtgcg
     2041 cagctcggct tcaatctggg gacggacgct ctcaagggtg ggcccggcgc ccggcgtgat
     2101 cttggtcacc ttgacgaccg agaggcccag attgccagtg atcggggggc tcacctgacc
     2161 gacggccagg ctgaaggccg cgtcggcgac cttgcggtcg ggcagggcgg acttgggctt
     2221 gtcgctaatg atgaccggct gcttgccata ggcgctggcc acgacggctg gctgatcgcc
     2281 cttcgccaga cgctgggcaa tcacggcggc cgccttggcg tcgggcgcca ggatctggac
     2341 cagcgaacgg gtttccggtg tggccagggt gtccttgcgg aaatcgaagg tcttctgcac
     2401 gtcggccgga ttgatcgtca ccgagggctc cagcgcctgg gcgctgacgc gcatgatcga
     2461 caggacgcgg gtctcgggcc gggtcagccg gtcggcattt tccttcatga aggtggtcaa
     2521 ctgggcgtcg gtcggatcgg tcggacgttc gatggtggcg ggattgatcg cgaaggccgc
     2581 cagatcgcgg ctctccagcc cataggccgc ttgtagcgcg gcatagatgc tgggcgcgtg
     2641 caggccattg gccacgcctg tgaagacatg ggtctgggcg atctcgtcgc tcagggacgc
     2701 ctgatagctg tccggcgtca ggccgttctc gcgaagcagg gcctcgtaca tcttgggatc
     2761 gaacttgccg gtgatcgggt cgaacggacg ctgattgggg tgcagattgc tcatctgctc
     2821 gcgcacgann nnnnnnnntt cgaccaccgc agccatggac tcctggaggg cgatttcctg
     2881 caggatctgg cgatcgacac cgcgctcgac ggcctggtca ggcgtcaggg cttcgccgga
     2941 gcgacgctcg gtttccttgc ggtagttttc gtatcgggtc ttgaaatcag tcgctgagac
     3001 cgtccgcgac cccgccgaga ccacacctga catgcgcggc ccgttgaaga cgtcgctgat
     3061 gccgaacacg gcgaagctga ggatcagcag gccaaacagg atcaccgcga agggtgattt
     3121 ggcgaaggtg cggaaaccgg cgagcatatt ttcgaagacc ttcctgactt gccgtatccg
     3181 cgcggccccg ggcctgaacc tggagcgaca cggcggacgc gagcagcggg tatagttgag
     3241 cctgtggcag gggggcaagc cacggcggtc ttgacttccc aagggacggc aagctcaaca
     3301 ccctattatg accctttcga gcctgacgcc ccggccgttg atcgccggaa actggaaaat
     3361 gaatggcctc gccgagagcc ttgatgaggc ccacgcgatc gtcgctggcc tcgacgccag
     3421 tcccgccgcc gcccgtgtcg cgatctgccc gccggcgacc ctgctgcacc gcctgagcca
     3481 ggcgctggag ggctctagcg tcctttccgg cgggcaggac tnnnnnnnnn ncgacatcgc
     3541 agcgtcgatg ctgatcgacg ccggcgccag cctggtgatc ctgggctact ccgaacgacg
     3601 cgtggatcat gacgagacca gcgcgctggt cgcggccaag gtcgaggcgg ccctggcggc
     3661 cggcctggag ccgatcatct gcgtgggcga aagcctggag cagcgcgagg cgggtgctgc
     3721 gctggaggtc gtgaccactc aggtccgcga atccttgccg ggcagtctgg cagggcaggc
     3781 cttctcggtg gcctatgagc cgatctgggc gatcggcacg ggccgcacgg cgacggtcgc
     3841 tgacatcgaa gacatgcacg cagccatccg cgcccagctg gtcgcgatgt ttggcgaggc
     3901 cggcaaggcg ccacccatcc tttatggagg ctcggtcaag cccgacaacg cggcggagat
     3961 cctgcgcgcg gccgaggtcg gcggcgcgct ggtcggcggc gcctcgttga aggccagcga
     4021 ttcc
//
LOCUS       DAOMEO010000146         4017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2658_length_4017_cov_2.077234, whole genome shotgun sequence.
ACCESSION   DAOMEO010000146 DAOMEO010000000
VERSION     DAOMEO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_4017_cov_2.077234"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLH31_04525"
     CDS             <1..489
                     /locus_tag="PLH31_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HQR88527.1"
                     /translation="WLDGLSASQIAKQLGGVTRNAVIGKVHRLGLSGRAAPSQPARPA
                     FKAPRPARPAAQTMPSAPRRVVTPEHTPVQAVVSSGVAPPTLPVYRDEQPGSATVLTL
                     GAHMCKWPIGDPSSDGFTFCGRRSSDGPYCVEHARVAYQPQQSKKKAGPTELARSLRR
                     YI"
     gene            567..>836
                     /locus_tag="PLH31_04530"
     CDS             567..>836
                     /locus_tag="PLH31_04530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR88528.1"
                     /translation="MTDVAAPDSNAPAYSVSELAFALKRTLETSYAHVRLRGELSKVT
                     HHGNGHVYLTIKDDKSAIDGVVWKGSVRGLGVRPEHGLEVIVTGKI"
     assembly_gap    837..846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <847..>1841
                     /gene="xseA"
                     /locus_tag="PLH31_04535"
     CDS             <847..>1841
                     /gene="xseA"
                     /locus_tag="PLH31_04535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR88529.1"
                     /translation="TTYPAGSRYQIVIESMEAAGVGALLAQLERLKAKLHGEGLFEAS
                     RKRALPSMPAVVGVITSPTGAVIRDILHRIRDRWPCHVLVWPVVVQGDQAAAQVAGAI
                     RGFNAITPGGRVPRPDIIIVARGGGSVEDLWAFNDETLARTVAASDIPLISAVGHETD
                     TTLIDFVSDRRAPTPTAAAEIATPVLGELRALVSDYDRRLNRCGSRVLEERRTRLVSA
                     ARALPRPADLLALAQQRFDIAAGRLDAALGRNTAVHAQDLLKITARLSPEALGRQHKV
                     KADRLGDLSRRMDLAARRAPERIAQNARLPALWSRLNDAGKRRLQRDADRLSGLE"
     assembly_gap    1842..1851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1852..2112
                     /locus_tag="PLH31_04540"
     CDS             <1852..2112
                     /locus_tag="PLH31_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR88530.1"
                     /translation="LEKLRQSLNPERPLELGFALVRKADGALARSAGDLAAGERVNLK
                     FKSGDRDAVIDGEGVVHAPPPPALKPTRAKPTTPKPDQGDLF"
     gene            2140..2361
                     /locus_tag="PLH31_04545"
     CDS             2140..2361
                     /locus_tag="PLH31_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HQR88531.1"
                     /translation="MNAYDRDLGKPDGLAVLHYGDGEFAVMSPGRFVLCAVTGAKVQL
                     EALRYWSPELQEAYATPADALKRWQEKIA"
     gene            2340..3194
                     /locus_tag="PLH31_04550"
     CDS             2340..3194
                     /locus_tag="PLH31_04550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR88532.1"
                     /translation="MAREDRLNRRALLAGLAVSAAAHAAWAAAPGLTLSGKFVQGGYA
                     IGQTAPHATIDVDGEVVGLASAEGWFVVGFDRDAPPIARVTVTTADGSASHEAAIAPG
                     TFDIQRIDGLPANQVAPVDPVLLARIAAEGARKAVGFASVADIDGFKSGFIMPVEGAR
                     RSARFGGQRVLNGEPKRPHFGVDLAAPVGTAVRAPASGLVVFAETGLHFEGGLILLDH
                     GQGLITAYLHLSKALVMAGDFVKQGQTIGAVGREGRATGPHLCWRMKWRGRNLDPSLM
                     VGASAPRL"
     gene            3299..3808
                     /locus_tag="PLH31_04555"
     CDS             3299..3808
                     /locus_tag="PLH31_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88533.1"
                     /translation="MSAEYATLRFLLVDDNHHMRAIVSAILKGVGVKHVREAMDGAEA
                     LQAMREGPIDIAIVDFKMSPLDGVQFTQLVRNSPDSVDPFLPIIMLTGFAERNRVFEA
                     RDAGVTEIVVKPVTARSLLDRINMVIYKPRPFIRSGDYFGPCRRRRDDPSYSGPRRRQ
                     GDGLGALEI"
     gene            complement(3805..>4017)
                     /locus_tag="PLH31_04560"
     CDS             complement(3805..>4017)
                     /locus_tag="PLH31_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HQR88534.1"
                     /translation="ARFRAVVHDPIVAPSTGDRTADIEAGVRLINSFMEERIRERPAE
                     WFWVHRRWPNEVYAALAAREARSEKG"
BASE COUNT          626 a         1454 c         1279 g          638 t
ORIGIN      
        1 tggctggacg gtctttccgc cagtcagatc gccaagcaat tgggcggcgt tacgcgtaac
       61 gccgtcatcg gcaaggtgca ccgcctgggc ctttcgggcc gcgcggcccc ctcgcaaccg
      121 gcgcgtccgg ccttcaaggc cccgcgtccc gcccgtcccg ccgcgcagac catgccgtcc
      181 gcgccgcgcc gggtcgtcac gcccgaacac acgccagtgc aggccgtggt gtcctccggc
      241 gtcgcgccgc cgaccctgcc cgtctatcgc gacgagcaac ccggttcggc caccgtgctg
      301 accctgggcg cccatatgtg caagtggccg atcggcgatc cctcgtcgga cggcttcacc
      361 ttctgcggcc gccgctcgtc ggatggcccc tactgcgtcg aacacgcccg ggtggcctac
      421 cagccgcaac aatccaagaa gaaggccggc cccaccgagc tggcccgctc gctgcgccgc
      481 tacatctagc gtctggcgaa cacccataag aacaagtctc tcatgcggcc ctccccgttc
      541 gtcggggagg gccttatgct gtcgccatga ccgacgtcgc cgcccctgat tccaacgcgc
      601 ccgcctattc cgtctcggag ctggccttcg cgctgaagcg cacgctggag accagctacg
      661 cccatgtccg gctgcgcggc gagctgagca aggtcaccca ccacggcaac ggccatgtct
      721 atctgacgat caaggacgac aagtcggcca tcgacggcgt ggtctggaag ggctcggtgc
      781 gcgggctggg cgtgcggccc gagcatggcc tggaagtgat cgtcaccggc aagatcnnnn
      841 nnnnnncacc acctatccgg ccggctcgcg ctaccagatc gtcatcgaga gcatggaggc
      901 cgccggcgtc ggcgcgcttc tggcccagct ggagcgcctg aaggccaagc tgcacggcga
      961 gggcctgttc gaggcctccc gcaagcgcgc cctgccctcc atgcccgccg tagtcggggt
     1021 catcaccagc ccgacgggcg cggtgatccg cgatatcctg caccgcattc gcgaccgctg
     1081 gccctgccac gtgctggtct ggccggtcgt cgtacagggc gaccaggccg ccgcccaggt
     1141 agccggcgcg atccggggct tcaacgccat cacgcccggc ggccgcgtgc cgcgtcccga
     1201 catcatcatc gtcgcccggg gtggcggatc ggtggaagac ctgtgggcct tcaacgacga
     1261 aaccctggcc cgcacggtgg cggccagcga catcccgctg atctcggcgg tcggccatga
     1321 gaccgacacc accctgatcg acttcgtctc cgaccgccgc gcgcccacgc ccacggcggc
     1381 cgcagagatc gccacgccgg tgctgggcga gttgcgagcc ctggtctccg actatgaccg
     1441 ccgcctcaat cgctgcggct cgcgggtgct ggaagagcgc cgtacgcggc tggtcagcgc
     1501 cgcccgcgcc ctgccccggc ccgccgacct gctggccctg gcccagcagc gcttcgacat
     1561 cgccgccgga cggctggacg ccgccctggg ccgcaacacc gccgtccacg cccaggatct
     1621 gctgaagatc accgcccgcc tgtcgcccga ggccctgggt cgccagcaca aggtcaaggc
     1681 tgaccgcctt ggcgacctca gccgtcgtat ggacctggcc gcccgccgcg ccccggaacg
     1741 catcgcccag aacgctcgcc tgcccgctct gtggagccgc ctcaacgacg ccggcaagcg
     1801 ccgcctgcag cgcgacgccg accggctgtc cgggctggaa annnnnnnnn nctggaaaag
     1861 ctgcgccagt cgctcaaccc cgaacggccg ctggagctgg gctttgccct cgtgcgcaag
     1921 gccgacggcg ccctggcgcg ctcagccggc gatctggcgg ctggcgagcg ggtcaatctg
     1981 aagttcaaga gcggcgaccg cgacgccgtg atcgacgggg aaggcgtggt ccatgcccct
     2041 ccgccgcccg ccctgaagcc cacgcgcgcc aagccgacaa caccaaaacc tgatcagggc
     2101 gatctgttct gagacgacgc tctggcgtag aataacccca tgaacgctta tgatcgtgat
     2161 ctcggcaaac ccgacggcct ggccgtgctg cactatggcg acggcgaatt tgccgtaatg
     2221 tcgcccggcc gtttcgtgct gtgcgccgtc accggcgcta aggtgcagct agaagccctg
     2281 cgctactgga gcccggagct gcaggaagcc tacgccactc ccgccgacgc gctgaagcgc
     2341 tggcaagaga agatcgcctg aaccgccgcg cgctcctggc aggcctcgcc gtcagcgctg
     2401 cggcccacgc ggcctgggcc gccgcgccgg gactgacgct gtcgggcaag ttcgtacagg
     2461 gcggctacgc catcgggcag accgcacctc acgccaccat cgatgtcgac ggtgaagtcg
     2521 tcggcctcgc ctcggccgaa ggctggttcg tggtgggctt tgaccgcgac gccccgccga
     2581 tcgcacgcgt cacggtgacc accgccgacg gttcggccag ccacgaggcg gcgatcgcgc
     2641 cgggaacgtt cgacatccag cgcattgacg gcctgcccgc caaccaggtc gcgccggtcg
     2701 atcccgtcct gctggcccgg attgccgctg agggcgcgcg caaggccgta ggtttcgcga
     2761 gtgtcgcgga tatcgacggc ttcaagagcg gcttcatcat gccggtcgag ggcgcgcgac
     2821 gttcagcgcg cttcggcggc caacgcgtac tcaatggcga gccaaagcgt ccccattttg
     2881 gggttgatct cgcggcgccc gtagggactg cggtgcgcgc gccagccagc ggattggtcg
     2941 ttttcgccga gaccggattg catttcgaag gcggtttgat cctgctcgat cacgggcagg
     3001 gcttgatcac ggcctatctt catctctcca aagcccttgt tatggctgga gattttgtca
     3061 agcagggcca gaccatcggc gccgtcggca gggagggacg cgctacgggt ccgcatctgt
     3121 gctggcgcat gaagtggcgt ggccgcaacc tcgacccatc cctgatggtc ggcgccagcg
     3181 ctcctcgcct gtagcgcgaa aataaaccat tcattagccg tgatctctct atccagaagc
     3241 tgggctacgt gcttactcgc ggctcggttt tccggtgtga tcaggagacg gccaccgcat
     3301 gtcggcggaa tatgcgaccc tgcgctttct gcttgtggac gataaccacc acatgcgggc
     3361 gatcgtcagc gccatcctca agggcgtggg cgtcaagcac gtgcgcgagg ccatggacgg
     3421 cgcggaggct ctgcaggcca tgcgcgaggg tccgatcgac atcgccatcg tcgatttcaa
     3481 gatgtcgccg ctggacgggg tgcagttcac ccagctggtc cgcaactcgc ctgacagcgt
     3541 cgacccgttc ctgccgatca tcatgctgac cggcttcgcc gaacgaaacc gcgtgttcga
     3601 ggcgcgtgac gccggcgtca ccgagatcgt cgtcaagcct gtcacggccc ggtcgctgct
     3661 ggatcgcatc aacatggtga tctacaagcc gcggcccttc atccggtcgg gggactattt
     3721 tggcccctgc cgccgccgcc gcgacgaccc cagctattcc ggccctcgtc gacgtcaagg
     3781 ggacggcctc ggcgcactgg aaatctagcc tttctccgac cgggcttctc tggccgccag
     3841 cgcggcatag acctcattgg gccaccgccg gtgcacccag aaccattccg ccggccgctc
     3901 gcgaatacgc tcttccatga aactgttgat caggcgcacc ccggcctcga tgtcagccgt
     3961 gcggtcaccg gtcgaaggtg cgacgatggg atcgtggacc acggcccgga accgcgc
//
LOCUS       DAOMEO010000147         4014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2662_length_4014_cov_2.468047, whole genome shotgun sequence.
ACCESSION   DAOMEO010000147 DAOMEO010000000
VERSION     DAOMEO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_4014_cov_2.468047"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLH31_04565"
     CDS             complement(<1..297)
                     /locus_tag="PLH31_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR88535.1"
                     /translation="MQRRPLGRSGLSTAPLIFGGNVFGWTADEATSHSLLDAFVAGGF
                     NAIDTADVYSAWVPGHEGGESESVIGRWLKARGRRDDVLILTKVAMWPRQPGLSA"
     gene            complement(321..1953)
                     /locus_tag="PLH31_04570"
                     /pseudo
     CDS             complement(321..1953)
                     /locus_tag="PLH31_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
     gene            complement(2067..2918)
                     /locus_tag="PLH31_04575"
     CDS             complement(2067..2918)
                     /locus_tag="PLH31_04575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR88536.1"
                     /translation="MSKPDTPAANAAQIDYWNAVVGRTWAQFQAQLDRQIAPLGAEAL
                     RVLAPAEGERILDVGCGCGETTLDLAARVGPAGQVTGVDISEPMLAVARSRERPAKGA
                     MPEFLCIDAQTADLGEATFDAVFSRFGVMFFADPTAAFANLHRAVRPGGRLAFVCWRP
                     YAENLWMRAPMDAAQPLLPPMPASDPLAPGPFAFADPQRLRSILEGAGFSGVRIDPFD
                     TSIGGASVKDTLSLTFQIGPLGSALRAAPHLAESVGEAVEAVLASHDTPQGVMMPACV
                     WIVQATR"
     gene            complement(3223..3750)
                     /locus_tag="PLH31_04580"
     CDS             complement(3223..3750)
                     /locus_tag="PLH31_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQR88537.1"
                     /translation="MTVYSLGAISPSLPAPGEYWIAPTASVMGNVILKKNASIWWGAI
                     ARGDNDPIEIGENSNVQDGSVLHTDHGSPLTIGSNVTIGHMVMLHGCTIGDGSLIGIG
                     SIVLNGARIGRNCLIGAGALITEGKDIPDNSMVMGAPGKVVREIGEQHALILQASALH
                     YVENWKRYDRDLRQL"
     gene            complement(3816..>4014)
                     /locus_tag="PLH31_04585"
     CDS             complement(3816..>4014)
                     /locus_tag="PLH31_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88538.1"
                     /translation="SLGQRGGDKTKTRAQRGGSALDREGGGSEERLNAKSDTLISVVG
                     SRDRTSTPVGIDGISPIESPD"
BASE COUNT          687 a         1382 c         1273 g          672 t
ORIGIN      
        1 ggccgacagg cccggctgcc ttggccacat ggcgaccttg gtcaggatca gcacgtcgtc
       61 gcgcctgccc cgggccttca gccagcggcc gatgacgctt tcggactcgc cgccctcatg
      121 gcccggcacc caggccgaat agacatcggc cgtatcgata gcgttgaagc cgccggcgac
      181 aaacgcgtcg agcagactgt gcgacgtcgc ctcatccgcc gtccagccaa agacattgcc
      241 gccaaagatc agcggggcgg tcgagaggcc ggagcggccg agggggcgac gctgcattca
      301 ctcacactcc caaagagcgg tcacgccgcg accgcgcgtc cgtccacgaa caccggcgcg
      361 tccatggccg gaagcatggc cttgcccaag agcaggtccg ctgcccgctc ggcgatcatg
      421 atggtggggg cgttggtgtt gccgcccacc agggtgggca tgaccgaggc gtcgatgacc
      481 ctcaggcccg ccacgccctg cacccgaagc tggtcgtcga ccaccgccat ggcgtcgccc
      541 gccgcgccca tccggcatgt gccgacgggg tgatagatgg tttccgacct ggcccggatc
      601 caggcgtcca gatccgcatc ggtccgcacg tcggcgccag gggcgtattc ggcgtcgcga
      661 taggggtcga gcgccgcctg ggcgacgacc ttgcgcgcca tgcggacgcc ctcgcgaagg
      721 gcgcggcggt cttcgtcggc ggccagatag ttgcccagaa tggtcggatc atcgaacgga
      781 tcggccgagc gcagacccac ggcgccgcgg ctctcggggc gcaactgaca gacgtgcagg
      841 gtgaagccgt ccttctcgat cagcgtcttg ccgtggtcct gcatgatggc cagcacgcca
      901 tggatctgca ggtcgggccg gtccagatcg ggccgtgact tcaggaaggc gccactttcc
      961 aggaactgct ggcgacccag gcccttgccc atcagcatgt aggacagccc cgtgcccagc
     1021 ttcctcaggc ccttgttggc cggacaggca gacgtcgagg tggtcctgca ggttggcgcc
     1081 gacacccttg gcctcctgca ccaccgccac gccatgcttc ctcagcaggt ccggcgcgcc
     1141 cacacccgac agctgcagga tctgcggcga ttgcaccgcg ccggcgctca gcagcacctc
     1201 ggcgtcggca tgggcggtgc gcttctcgcc gcccttgccc accacatagt caacgccgat
     1261 ggcgcggccc ttctcgatca ggatcctggt cgtgcgggct tctgtgacgc aggtcaggtt
     1321 gggccgcgcc agcgccgccg tcagataggc cgccgccgcg ctccagcgct tgccgtcgcg
     1381 gatggtcagg tcgtaggggc caaagccctc ctgctgatag ccgttgaagt cgcgggtggt
     1441 cttgtagccc gcctggcgcc ccgcctcgat cagggccgga tagaagggac tgggcgactc
     1501 gcccttggac acatgcagcg gcccctgccc gccatgatag gcgtcgccgc cgccatggtg
     1561 ggtttccgaa cgcttgaagt agggcaacac ctccgaatag gaccagcccg acagacccat
     1621 ctgccgccac tggtcatagt cgcgcgcatg gccgcgaata tagatcatgc cgttgatcgc
     1681 cgaggacccg cccaggccct tgccgcgcgg ccaccacagc ttgcggttat caaggtgggg
     1741 ctcctcctcg gtccagaagc cccagttttg ctcgcccttg gccttgatca gctcgcccac
     1801 gccggccggc atcttgacca ggatggaggt gttcttgccg ccggcctcca gcagcagaac
     1861 cttgatattg gggtcttccg tcagacgcgc agccagaacg catcccgccg aaccggcgcc
     1921 gatgacgatg taatcgaagc gttcgctagc catggccccg acgccctccc tgaatgttat
     1981 cgttgttaag acagagattg gctcggcttt tcccccggcg caaggcgcag tatgcgcgcc
     2041 tttccaggcg cgcagcccgc agccgtctag cgtgtggcct gcacgatcca gacacaggcc
     2101 ggcatcatga cgccctgggg cgtatcatgg ctcgccagaa ccgcctccac ggcctcgcca
     2161 acgctttccg cgaggtgcgg cgccgccctc agggccgacc ccaggggccc gatctggaag
     2221 gtcaggctga gcgtgtcctt gaccgaagcg ccgccgatcg acgtgtcgaa cgggtcaata
     2281 cgtacgccac tgaagcccgc gccttccagg atcgaacgca ggcgctgcgg atcagcaaag
     2341 gcaaaaggtc ccggcgcaag cggatcggag gccggcatcg gcggcagaag cggctgggcg
     2401 gcgtccatgg gggcccgcat ccacaggttt tccgcatagg gtcgccagca gacaaaggcc
     2461 aggcgtccgc ccggccgcac cgcccgatgg aggttggcga aggccgccgt cgggtcggcg
     2521 aagaacatca cgccaaacct tgagaacacg gcgtcgaagg tcgcctcacc cagatcagcg
     2581 gtctgggcgt cgatgcacag gaactcgggc atggcgccct tggccggacg ctcacgcgac
     2641 cgggccaccg ccagcatggg ttctgaaata tcgacgcccg tgacctgccc ggcaggtccg
     2701 acacgtgcgg ccagatcgag ggtggtctcg ccacagccgc aaccgacatc cagaatccgc
     2761 tcgccttcag caggtgccag aacccgcagg gcctcggcgc cgagaggcgc gatctggcgg
     2821 tccagttgag cttgaaactg ggcccaggtt cgaccaacca cggcgttcca gtagtcgatc
     2881 tgggcggcgt tggcggccgg ggtatcaggt ttggacaaag ggtctcctcg gcggcggcgc
     2941 cgcgcaattg atataaaggg gctttcctcc gatcccagac gccatagccg gcccgcgcgc
     3001 gcaggcgcct cagccccttg cgcggcgatg gccctagcgc caggggcgca tgcgacgtcc
     3061 cgcagtagga tatgtctctc agcaagggac gtcatgattg gtcacccccc gcgtgatcag
     3121 acgtaggaga ccataggcgg gggtcaaggc cctaaggcgc cccagggtcg cccccactga
     3181 ccgccagcct gatctgcaag gcccttgggc gccaatcccg gctcagagtt gacggaggtc
     3241 gcggtcgtaa cgcttccagt tctcgacata gtgcagggca gaagcctgca ggatcagggc
     3301 gtgctgctcg ccgatctcgc gcaccacctt gccgggcgcg cccatcacca tcgaattgtc
     3361 cgggatgtcc ttgccctcgg tgatcagggc cccggccccg atcagacagt tcctgccgat
     3421 cctggcaccg ttgagcacaa tcgatccgat tccgatcagc gagccgtcac cgatggtgca
     3481 accatgcaac atcaccatgt gaccgatggt cacattggac ccgatcgtca gcggagagcc
     3541 atggtccgtg tgaagtactg aaccgtcctg cacattgctg ttctcgccga tttcgatcgg
     3601 atcgttgtcg ccccgggcaa tggcgcccca ccaaatactg gcgtttttct tgaggatgac
     3661 attgcccatg accgatgcgg tcggcgcgat ccagtattcg cctggcgcgg gaaggctcgg
     3721 gctgatggcg cccaaggaat agacagtcat ccgtacaccc cgtgttcttg aaatcgtcgc
     3781 ttgattaaca tctgagaaac cacgttagca tcatcctagt cgggcgattc gattggagaa
     3841 atcccatcga ttcccaccgg ggtcgatgtt cggtcccggc tcccgacgac ggagatcagg
     3901 gtgtcgctct tggcgttcag gcgttcctcg gaaccaccgc cttctcggtc cagcgccgag
     3961 ccgccgcgtt gcgcacgcgt cttcgtctta tctcctcccc gctgccccaa ggac
//
LOCUS       DAOMEO010000148         4006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2672_length_4006_cov_2.683624, whole genome shotgun sequence.
ACCESSION   DAOMEO010000148 DAOMEO010000000
VERSION     DAOMEO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_4006_cov_2.683624"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>952)
                     /locus_tag="PLH31_04590"
     CDS             complement(<1..>952)
                     /locus_tag="PLH31_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQR88539.1"
                     /translation="GKTVVAMLAVADAAGAGLQSAIMAPTEILARQHYETMSGPLSAL
                     GLSVILLTGRDKGAGRAAKLAGLASGQHAVAIGTHALFQDDVVFKALGLSIIDEQHRF
                     GVNERRRLQDKGDGVHLLAMSATPIPRTLELTVFGDLDVSRIDEKPPGRTPVATRAVP
                     MPRLPEIVARLKAAAAGGAQAFWICPLVSESEKIDLRAAEDRAADLRQIIGSSVGLVH
                     GQMPAAEKDAVMADFVDGKVSVLVATTVVEVGVNVPNASIMVIEQAERFGLAQLHQLR
                     GRVGRGARESSCVLLYDLPLSDTAQQRLDILRKSDDGFEIA"
     assembly_gap    953..962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<963..>1353)
                     /locus_tag="PLH31_04595"
     CDS             complement(<963..>1353)
                     /locus_tag="PLH31_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQR88540.1"
                     /translation="GEIPDFEPVYPATAGLPSRTFRRFALEALSRAAPLPEWLDPAWQ
                     AREGLPSWHDAFVATHNPTGEADVSPLSPHRRRLAYDELLAHQLALAQRKASRRARPA
                     PRIPASPLAEDAEKALPFRLTGAQIRSL"
     assembly_gap    1354..1363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1364..1781)
                     /locus_tag="PLH31_04600"
     CDS             complement(<1364..1781)
                     /locus_tag="PLH31_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQR88541.1"
                     /translation="MRPEILFPLFTPVSTLKGVGPRVAPLVERLAGPIVRDVLFTVPT
                     SVIRRIATTVDRAVDGQVQTFIVSIDAHQPPSRLGQPWKIRAWDETGFLTLTWFKGHG
                     PHLERQHPPGSRRAVSGKVERPERFASEFQMAHPEYI"
     gene            complement(1826..2128)
                     /locus_tag="PLH31_04605"
     CDS             complement(1826..2128)
                     /locus_tag="PLH31_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88542.1"
                     /translation="MKPYIELKGASGAVYRYKLAEDADPRTTIAGNFVYLDAKGAVLF
                     AGEANNLIGATARWSEAVSRHGAATLYTRLNVSGASRAEEHADLVTALDPVMNRDA"
     gene            complement(2199..2933)
                     /locus_tag="PLH31_04610"
     CDS             complement(2199..2933)
                     /locus_tag="PLH31_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQR88543.1"
                     /translation="MSAVSARGLVKRFDDVVVLNGVDIEVAQGEVVAIIGPSGSGKST
                     LLRCMAGLELLDGGALRIAGVEAAPRAPLARALKGRVGFVFQSFNLFPHRTALQNVAE
                     GLIVVRGQPRAAAEAKARALLEKVGLGARVDAWPGELSGGQQQRCAIARALAMDPEVI
                     LFDEPTSALDPELVGEVLSVIRELAAEKRTMLIVTHQMDFARDVADRTIFMDSGVIVE
                     QAPSRELFGAPKEERTRRFLGKVAAP"
     gene            complement(2930..>3219)
                     /locus_tag="PLH31_04615"
     CDS             complement(2930..>3219)
                     /locus_tag="PLH31_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR88544.1"
                     /translation="LGMSRGQALRRVILPQAARTAVAPLSNSFISLVKDTSLAATIQV
                     PELFRQTQLITARTFEIFTMYLAAAALYWIVSTLLAAGQGVLERRAAEGRR"
     assembly_gap    3220..3229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3230..3487)
                     /locus_tag="PLH31_04620"
     CDS             complement(<3230..3487)
                     /locus_tag="PLH31_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516880.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR88545.1"
                     /translation="MDATLDLLSQSAPLLLKGAGYTIALSLIGMSLGLVLGFGLALMR
                     LSRSALLRWPAGVYVSAIRGTPLLVQLFLIYYGLPQFGVELP"
     gene            complement(3490..>4006)
                     /locus_tag="PLH31_04625"
     CDS             complement(3490..>4006)
                     /locus_tag="PLH31_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQR88546.1"
                     /translation="LSRAWFGAVNVVINQITITPERAAKYDFSTPYTVSGIQIIVPRG
                     QAGPYSPAELAGKKVGVGLGTNYESWARANVPTADIRTYDDDPTKYQDLKAGRIDAVL
                     NDRLVAADFVKNSPEFIAAGQAFAPQNQGVAMLKDPAMKAMVDQAIAAMRADGRLEAI
                     SRQWFGADVTQ"
BASE COUNT          638 a         1311 c         1436 g          591 t
ORIGIN      
        1 gcgatctcga agccgtcgtc ggacttgcgc aggatgtcga gcctctgctg ggcggtgtcg
       61 gacagcggca ggtcgtacag cagcacgcag gagctttctc gcgcgccccg gccgacgcgg
      121 ccgcgcagct ggtgcagttg ggccaggccg aaacgctcgg cctgctcgat gaccatgatg
      181 ctggcattgg ggacgttgac ccccacctcg accacggtgg tggccaccag caccgagacc
      241 ttgccgtcga cgaagtcggc cataaccgca tccttctcgg cggcgggcat ctggccgtgc
      301 accagaccga cggaggagcc gatgatctgg cgcaggtcgg cggcgcgatc ctcggcggcg
      361 cgcaggtcga tcttctcgga ctccgagacc agcgggcaga tccagaaagc ctgggcccct
      421 ccggcggcgg cggccttcag gcgggcgacg atctcgggca ggcgcggcat gggcacggcg
      481 cgggtggcga ccggcgtgcg acctggcggc ttttcgtcga tgcgcgagac gtccagatcg
      541 ccgaacaccg tcagttccag ggtgcgcggg atcggcgtgg ccgacatcgc cagaagatgc
      601 accccgtctc ccttgtcctg cagccggcgg cgttcgttga cgccaaagcg gtgctgctcg
      661 tcgatgattg acaggcccag ggccttgaac accacatcgt cctggaacag ggcgtgggtg
      721 ccgatcgcca cggcgtgctg gccgctggcc aggccggcca gcttggcggc gcggccggcg
      781 cccttgtcgc gtcccgtcag caggatcacc gacaggccca aggccgacag cggcccggac
      841 atggtctcat agtgctggcg ggccaggatc tcggtgggag ccatgatcgc cgattgcagg
      901 cccgcgccgg ctgcgtcggc cacggccagc atggcgacca cggtcttgcc cgnnnnnnnn
      961 nngaggctgc gaatctgggc cccggtcagc cggaagggca gggccttttc ggcgtcctcg
     1021 gccagggggc tggcggggat gcgcggcgcg ggtcgggcgc ggcgcgaggc cttgcgctgg
     1081 gccagggcca gctgatgggc cagcagttcg tcataggcca ggcgccggcg atgcggcgac
     1141 aggggcgaga cgtcggcctc gccggtggga ttgtgcgtgg cgacgaaggc gtcatgccag
     1201 ctgggcaggc cttcgcgggc ctgccaggcc gggtccagcc actcgggcaa gggcgcggcg
     1261 cggctgaggg cttccagggc gaaccgtcgg aaggtgcgcg agggcaggcc ggcggtggcg
     1321 ggatagacgg gctcgaaatc cgggatctct cccnnnnnnn nnngaatgta ttccggatgc
     1381 gccatctgga attcggaggc gaaccgctcg ggccgctcga ccttgccgct gacggcacgg
     1441 cgtgatcctg gcggatgctg gcgctccagg tgcggtccgt ggcccttgaa ccaggtgagg
     1501 gtcaaaaagc cggtctcgtc ccaggcccgg atcttccacg gctgtcccag ccgcgagggc
     1561 ggctggtggg cgtcgatgct gacgatgaag gtctggacct ggccgtcgac ggcgcggtcc
     1621 accgtggtgg cgatacgccg gatcacgctg gtggggacgg tgaacagcac gtcgcggacg
     1681 atggggcccg ccaggcgctc gaccagaggc gccacgcgcg gccccacgcc ctttagcgtg
     1741 gagaccggcg taaacagcgg gaagagaatc tcgggtcgca tctggacacg accatagcca
     1801 agtcggcgtc agaaggggac gccgcttagg cgtcccggtt catcaccgga tcgagggcgg
     1861 tgacaagatc ggcatgctcc tcggcgcgcg aggcgcccga gacgttcagg cgcgtataga
     1921 gcgtggccgc gccatggcga ctgacggctt ccgaccagcg cgcggtcgcg ccgatcaggt
     1981 tattggcctc gccggcaaac agcacggcgc ccttggcatc gagatagacg aaattgccgg
     2041 caatggtcgt ccggggatcg gcgtcctcgg ccagcttgta gcggtagacg gcgcccgagg
     2101 cgcctttcag ttcgatatag ggtttcacag gacctcgcct caactgcacg cggcgttggc
     2161 ggcgatgtgg tggagggcgc cgaaggccaa attatggctc agggcgccgc cactttgccg
     2221 aggaatcgtc gcgtgcgctc ctccttaggc gctccgaaca gctcgcgcga gggggcctgt
     2281 tcgacgataa cgccgctatc catgaagatc gtccgatccg ccacatcccg ggcgaagtcc
     2341 atctggtggg tgacgatcag catcgtacgc ttctcggcgg ccagttcgcg gatcaccgac
     2401 agcacctcgc cgaccagttc cggatcaagg gcggaggtcg gttcgtcgaa caatatgacc
     2461 tccggatcca tggccagggc ccgggcgatg gcgcagcgtt gctgctggcc gccggacagc
     2521 tcgccgggcc aggcgtcgac ccgcgcgccc aggccgacct tttccagcag ggcgcgggcc
     2581 ttggcctcgg cggcggcgcg gggctggccc cgcaccacga tcaggccctc ggcgacgttc
     2641 tgcagggcgg tgcggtgcgg aaacaggttg aagctctgga agacgaagcc cacccggccc
     2701 ttcagggcgc gggccagcgg cgcgcggggc gcagcctcga cgccggcgat ccgcaaggcg
     2761 ccgccgtcca gcagttccag tccggccata cagcgcagca gggtcgactt gcccgagccg
     2821 ctgggaccga tgatggccac cacctcgccc tgagcgacct caatatcgac gccattcagc
     2881 accacgacat cgtcgaagcg cttgaccagg ccccgggcgg aaacggcgct catcggcggc
     2941 cctcggcggc gcggcgttcc agcacgccct ggccggcggc cagcagggtc gagacgatcc
     3001 agtagagggc ggcggccgcc agatacatcg tgaagatctc gaaggtgcgg gcggtgatca
     3061 gctgggtctg gcgaaacagc tccggcacct ggatcgtggc ggccagggag gtgtccttga
     3121 ccaggctgat gaagctgttg gacagcggcg cgacggcggt gcgggcggcc tgcggcagga
     3181 tcacccggcg cagggcctgg ccgcggctca tgcccagcan nnnnnnnnnc ggcagctcga
     3241 ccccgaactg cggcaggccg taatagatca gaaacagctg caccagcagc ggcgtgccgc
     3301 gaatggccga cacataaacg cccgccggcc agcgcagcag ggccgaacgc gacagccgca
     3361 tcagggccag accaaagccc aacaccagcc ccaggctcat gccgatcaga ctcagcgcta
     3421 tcgtgtagcc cgcgcccttc agcagcagcg gcgctgactg gctcagcaga tccagggtgg
     3481 cgtccatgcc tactgagtca cgtcggcgcc aaaccactgc cgggagatcg cctcaaggcg
     3541 gccgtcggcg cgcattgcgg cgatggcctg gtcgaccatc gccttcatgg ccggatcctt
     3601 cagcatggcc acgccctggt tctgcggcgc aaaggcctgg cccgcggcga tgaactctgg
     3661 cgagttcttg acgaagtcgg cggccaccaa gcggtcgttc agcaccgcgt cgatgcggcc
     3721 ggccttcagg tcctggtact tggtaggatc gtcgtcatag gtgcggatgt cggcggtcgg
     3781 cacattggcc cgcgcccagc tctcatagtt ggtgcccagg ccgacgccca ccttcttgcc
     3841 ggccagttcg gcaggcgaat aggggccggc ctggcccctc ggcacgatga tctggatgcc
     3901 cgagaccgtg tagggcgtgg aaaagtcgta cttggcggcg cgctcgggcg tgatggtgat
     3961 ctggttgatc accacgttca ccgcgccgaa ccaggcgcgg ctgagg
//
LOCUS       DAOMEO010000149         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2673_length_4005_cov_2.646835, whole genome shotgun sequence.
ACCESSION   DAOMEO010000149 DAOMEO010000000
VERSION     DAOMEO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_4005_cov_2.646835"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..193)
                     /locus_tag="PLH31_04630"
     tRNA            complement(119..193)
                     /locus_tag="PLH31_04630"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Asn,seq:gtt)
     gene            485..1057
                     /locus_tag="PLH31_04635"
     CDS             485..1057
                     /locus_tag="PLH31_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HQR88547.1"
                     /translation="MSTDFAAARLNMVESQIRTADVTDLPLQDALRVVAREALVPASK
                     AYLAYADAEVEYAPGRWLLKPRDVGKLLQFLKPRAGEKALAIAAPYAAAVLETMGLSV
                     TRLEGDDLKSPAGDGYDVIICEGAVLAAPAAWTEVLAVGGRLGVVERSGPVGQATVHV
                     RAEDGVGRRAVFDATPPILAGFSAEPGFAF"
     gene            1211..2647
                     /locus_tag="PLH31_04640"
     CDS             1211..2647
                     /locus_tag="PLH31_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQR88548.1"
                     /translation="MSNSRRAGLLAAACSLGLMAGFVSAAQAETLTDAIALAYQTNPT
                     LQQQRATQRATDESVVQAKTAFRPTASISADITESRSDPFGGTRVEASGSGATLSISQ
                     PLYTGGRASANLTAAEADVLAAREGLRNIEQNVLINVIQAYVDVRRDEERLRISQENV
                     SVLGRQLDESNARFEVGEITRTDVAQSQARRAAAQAALSSAQAQLSISRASYAAIVGQ
                     NPTDLVAEPSLAALLPSTVEQAFEAAENNNPQVAAARFNERAAAARLASVRASRRPTV
                     SARASLGYDASEVNGAGDQFADYDRQLTGAVTASIPLFTGGLTSSQVRAAIERENAAH
                     IAVEGAKRTALQSVSNAWSNLLASRSNLVSNEEQVRAARIAFEGVRQEQQVGLRTTLD
                     VLNAQQELRAAELALVSARRDEYVASASVLQAMGALDVAALAPDVARYDPKTSYDRVN
                     RAIGWVPWEPVVQVIDKVGAPSTKPSSK"
     gene            2777..3319
                     /locus_tag="PLH31_04645"
     CDS             2777..3319
                     /locus_tag="PLH31_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HQR88549.1"
                     /translation="MSDQSSQEPTMEEILASIRPSISEDDAPAEPEAAAPAPAPEPEP
                     EYQAPAEDDVLELTDPIEPEPAHEPEPAPLESVGDLDVYSPAAPEPEPAYTPPPAPSF
                     NRDDVAETLVGDHASSLAASAFGSLSSALLMPKDGRTLEDVVRELLRPLLKEWLDQNL
                     PRIVETKVEEEVHRIARGRV"
     gene            3471..>4005
                     /locus_tag="PLH31_04650"
     CDS             3471..>4005
                     /locus_tag="PLH31_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR88550.1"
                     /translation="MLDKTFDPKAVEPRLYAAWEASGAFKPSEDPNAEPFVIVIPPPN
                     VTGSLHIGHALNNTLQDVLTRFHRMRGKAALWLPGTDHAGIATQMVVERQLAAAGNVG
                     RREMGREAFVEKIWEWKAESGGTIVNQLRRLGASCDWSRERFTLDEGLSAAVRKVFVQ
                     LYKQNLLYRDKRLVNWDP"
BASE COUNT          691 a         1354 c         1300 g          660 t
ORIGIN      
        1 gtccacatcc tcatactgag gaaatgacgc gcgccggggg cgacggccga ggctcgaaaa
       61 gtttgaaggg ggagagcgcg acctccgccg atgcgaaggt cgctgctcaa gccagaggtg
      121 gctccgcggg taggattcga acctacgacc agccggttaa cagccggctg ctctaccact
      181 gagctaccgc ggaacaggtc gcctcaggaa gaggccgggt gatagccgcc tcattgactc
      241 ggcgcaagcg ccttcgaaca aaaaaacagc ccggcttgtg accgggctgt ggaaagtcag
      301 tgatggtggg gtgtcttcga gaagaccccg gaaaattgcg tgtttatggt taatcgggcc
      361 ttaatttcag gcggggccaa aaggtcgcag ccgatccctc ctcgtcttcg caagcgttcg
      421 cattgccttg aggcaagggg cggacctata agcctgcgac cgtcaagtcc gaccgaggga
      481 ttccatgagc accgatttcg ccgccgcgcg gctgaacatg gtggagagcc agatccgcac
      541 cgcggacgtc accgacctgc ccttgcagga cgcgctgcgc gtcgtggccc gcgaggccct
      601 ggttccggcc agcaaggcct atctggccta tgcggacgcc gaggtcgaat atgcgccggg
      661 acgttggctg ctgaagccgc gtgatgtcgg caagctgctg cagtttctga agccgcgcgc
      721 gggtgaaaag gccctggcca tcgccgcgcc ctacgcggcg gcggtgcttg agaccatggg
      781 cctttcggtg acacggctgg agggcgatga cctgaaaagc ccggccggcg atggctatga
      841 cgtcatcatc tgtgagggag cggttcttgc ggcgcccgcc gcctggactg aggtcttggc
      901 ggtcggcgga cgtttagggg tggtcgagcg tagcggcccc gtgggccagg caaccgtcca
      961 tgttcgcgct gaagatggcg tcgggcggcg cgccgtgttc gacgccaccc cgcccatcct
     1021 ggcgggtttc tcagccgagc cgggatttgc gttctagcct gaggtcgttg tcgagcgttt
     1081 gtcgttgtag aaacatgcgc gcaacgtgtg cgtgaaaaaa gcttaactag cggccgctgg
     1141 cggtcagcct tacacgcgct catatagcag gtgcgggcgt gagcttcgcg tgtcttgggg
     1201 atagaacttg atgtcgaata gccgtcgggc cggattgttg gccgccgctt gcagcttggg
     1261 cctgatggcc ggcttcgtgt ccgccgccca ggccgagacg ctgaccgacg ccatcgccct
     1321 ggcctatcag accaacccca cgcttcagca gcagcgcgcc acccagcgcg ccaccgatga
     1381 gagtgtcgtt caagccaaga cggcgttccg gccgaccgcc agcatctcgg cagacatcac
     1441 cgagagccgg agcgatccct ttggcggcac gcgcgtcgag gcctccggca gtggcgcgac
     1501 cctgtccatc agtcagccgc tctatacggg cggccgggcc agcgccaatc tcaccgccgc
     1561 cgaggctgat gtgctggcgg cccgcgaagg cctgcgcaat atcgaacaga atgtcctgat
     1621 caacgtgatc caggcctatg tggatgtgcg tcgcgacgaa gaacgcctgc ggatctctca
     1681 ggagaacgtt tcggtgctcg gccgacagct ggacgagtcc aacgcgcgct tcgaagtggg
     1741 cgagatcacg cgaaccgacg tcgcgcagtc ccaggctcgc cgcgcagcgg cccaggccgc
     1801 tctcagttca gcccaggccc agctgtcgat cagccgcgcc agctatgcgg ccatcgtcgg
     1861 ccagaacccg accgatctgg tggccgagcc ctcgctcgcc gcgttgctgc cgtcgaccgt
     1921 cgagcaggcc ttcgaggccg ccgagaacaa caatcctcaa gtcgcggccg cgcgcttcaa
     1981 cgaacgcgca gcagcagccc ggctggcctc cgtccgcgct tcgcgtcggc ccaccgtttc
     2041 ggcccgggct tcgctgggtt acgacgccag cgaggtcaat ggcgccggcg accagttcgc
     2101 agactatgac cggcagttga ccggggctgt caccgcctcc atcccgctgt tcacgggggg
     2161 gctgacctcg tctcaggttc gcgccgctat cgagcgcgaa aacgccgccc atatcgccgt
     2221 ggaaggcgcc aagcgcaccg ccctgcagtc ggtctccaac gcctggagca acctgctggc
     2281 ttcgcgttcc aacctggtct cgaacgaaga acaggtccgc gccgcccgca tcgccttcga
     2341 gggcgtgcgt caggaacaac aggtcggttt gcgcaccacg ctcgacgtcc tgaacgccca
     2401 gcaggaattg cgcgccgccg agctggccct ggtatcggcc cgccgcgacg aatatgtcgc
     2461 cagcgcctcg gttcttcagg ccatgggcgc actggatgtt gcagccctgg ctcccgacgt
     2521 cgcccgctat gaccccaaga cctcatacga ccgcgtcaac cgtgcgatcg gctgggtgcc
     2581 gtgggagccg gtggttcagg tgattgacaa ggtcggagcg ccgtccacga agccctcttc
     2641 gaaataggct ttgccgcgtc gatttggccc acccgcaatc cttgcgcttg cttaagccgc
     2701 gcaagtctgc accatcggcc cttagcgctc ttcggggcgc gtcaaggatt cgtacctctt
     2761 tttgacggcc ccgcacatgt ccgaccagag ctctcaagaa ccgacgatgg aggagatcct
     2821 cgcctccatc cggccgagca tctccgaaga tgacgcgccg gcggagcccg aggcggccgc
     2881 gcctgcgccg gcgcccgaac cggaacctga atatcaggcc ccggctgagg acgacgtgct
     2941 ggagctgacc gatccgatcg aacccgagcc agcccatgag cccgagccgg ctccgctcga
     3001 aagcgttggc gatctggatg tctattcccc ggctgcgccc gagcccgagc cggcctatac
     3061 gccgccgcct gcgccgtcgt tcaaccgcga tgacgtggcc gagaccttgg tcggcgatca
     3121 cgcctcgagc ctggccgcca gcgccttcgg cagcctgagc tcggccctgc tgatgcccaa
     3181 ggacggccgc accctggaag acgtggtgcg cgaactgctg cgcccgctgc tcaaggagtg
     3241 gctggaccag aacctgccgc gcatcgtcga gaccaaggtc gaagaagaag tccaccgcat
     3301 cgcgcgcggc cgcgtataaa atcgggtctc gcgtcggctc tggacggcgc gcagacctcg
     3361 aaacctgaaa gggcggtcgc gatggcggcc gcctttttcc gttcaaatcg ttcctgaatc
     3421 caggccgccc ggtttcgcgc ggcggccgca gatggcatac ccgcaccgcc atgcttgaca
     3481 agaccttcga tcccaaagcc gtcgaacccc gcctgtacgc cgcctgggag gcctccgggg
     3541 ccttcaagcc gagcgaggat ccgaacgccg agccgttcgt gatcgtcatc ccgccgccga
     3601 acgtgacggg ctcgctgcac atcggccatg cgctgaacaa caccctgcag gacgtgctga
     3661 cgcgcttcca ccgcatgcgc ggcaaggccg ccctgtggct gccgggcacc gaccacgcgg
     3721 gcatcgccac ccagatggtg gtcgagcgcc agctggccgc cgccggcaat gtgggccgtc
     3781 gcgagatggg ccgcgaggcc ttcgtcgaga agatctggga atggaaggcc gagagcggcg
     3841 gcacgatcgt caaccagctg cgtcgcctgg gcgccagctg cgactggtcg cgcgagcgct
     3901 tcaccctgga cgagggcctg tcggccgccg tccgcaaggt gttcgtccag ctctacaagc
     3961 agaacctgct gtatcgcgac aagcgcctgg tcaactggga cccgc
//
LOCUS       DAOMEO010000150         3975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2688_length_3975_cov_2.408929, whole genome shotgun sequence.
ACCESSION   DAOMEO010000150 DAOMEO010000000
VERSION     DAOMEO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2688_length_3975_cov_2.408929"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..922
                     /locus_tag="PLH31_04655"
     CDS             53..922
                     /locus_tag="PLH31_04655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR88551.1"
                     /translation="MAPKKKSGRSPPGPLEYLRRWQMQAWLAAVAIFLYAPLIALMAF
                     SFNDSRRNIVWNGFTLKYYEKAFNNAGLIEAFGNSLTIAAVSTVVSVVLGAMVALALW
                     RFRFPGKTALNGALALPIVVPEICMGVAMLVFFARVMPWPQGMVWPLNLGAIIIAHVS
                     FSFPFVAVVVRARMTSFNREMEEAARDLGAGEWRTIRDVILPHMAPSLVAGALLAFTL
                     SLDDFVITFFTAGPDTVTFPVKVYSMVRFSVTPEVNAASTILIVLTVILTAVALKLQG
                     NSAATAGHGGDKS"
     gene            964..2031
                     /locus_tag="PLH31_04660"
     CDS             964..2031
                     /locus_tag="PLH31_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR88552.1"
                     /translation="MVAVDNVSLSVEEGEFFSLLGPSGCGKTTLLRMLAGFETPTEGR
                     ILIDGQDVSTVPPNKRPVNMVFQSYAVFPHMTVADNVAYGLKVDRVPKAERDRRVEEA
                     LELVQLGGLGGRKPDQLSGGQRQRVALARALVKRPRVLLLDEPLSALDAKLREQMRTE
                     LCTLQEKVGITFIMVTHDQDEALALASRCAVMNRGVLQQVATPSDLYEFPNSRFVADF
                     IGSVNLFEGVLAVDEPSHAVIRSPDLPVDIFLDHGVTGPRGGAAWAAIRPEKIELHKK
                     DGDEAPNLGDAPKGANAVAGVIKHEAYLGGSSVYEVEIAGGRRIKVHRSNLTRWDQED
                     FKLGEEVWLCWHACSPAVLLS"
     gene            2261..2393
                     /locus_tag="PLH31_04665"
                     /pseudo
     CDS             2261..2393
                     /locus_tag="PLH31_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478446.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
     assembly_gap    2394..2403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2404..2918
                     /locus_tag="PLH31_04670"
     CDS             <2404..2918
                     /locus_tag="PLH31_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077994.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQR88553.1"
                     /translation="DAPGPFDLARDTMTADLIKAMLDLGRPVFGVCRGFQEINVAFGG
                     TLRRDMASSAELIAHHAPDEVDFNGMFDHRHPVDLTPGGVLATAFGASSAVVNSVHYQ
                     GVDRLGEGLTVEARAPDGVVEAVSASVNGAPVLAVQWHPEWKPDQNVQSQIFFQLFGR
                     ALRGAPLLVA"
     gene            2928..>3323
                     /locus_tag="PLH31_04675"
     CDS             2928..>3323
                     /locus_tag="PLH31_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR88554.1"
                     /translation="MTVPIRNHDIAELKRLDLAHHLPAQTDYKLQHDLGGSRIITRAD
                     GVYIHDGEGHQILDGMAGLWCVNVGYGRQELAQAAYEQMLELPYYNTFFKTASPPPIR
                     LAAKIAEKMGGHLTHVFFNSSGSEANDTVF"
     assembly_gap    3324..3333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3334..>3975
                     /locus_tag="PLH31_04680"
     CDS             <3334..>3975
                     /locus_tag="PLH31_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR88555.1"
                     /translation="KTKGQPQRTAFISRWNAYHGSTVAGVSLGGMKHMHVQGDLPIPG
                     VHHVMQPYSFNEGFDEDPAAFCARAVKEIEDKILEVGPETVAAFIGEPVQGAGGVIIP
                     PDGYWPAVEAVCRKYGILLVCDEVICGFGRLGQWFGHQHYGIKPDMIAMAKGLSSGYL
                     PISAVGVADFIVAELKEKGGDFIHGFTYSGHPTACAVALKNIEILEREGLVERT"
BASE COUNT          632 a         1298 c         1336 g          689 t
ORIGIN      
        1 tcgccatcgc cctgcaggcc gtgctgtctc gcaagaagcc tgaggcgaag tgatggcccc
       61 caagaaaaag tcggggcgct ccccccctgg tccgctggaa tatctccgcc gctggcagat
      121 gcaggcctgg ctggcggccg tggcgatctt tctctacgcg ccgctgatcg ccctcatggc
      181 cttcagcttc aatgacagcc gccgcaacat cgtctggaac ggcttcacgc tgaagtacta
      241 cgagaaggcc ttcaacaatg ccgggctgat cgaggcgttc ggcaattccc tgaccatcgc
      301 ggcggtctcc accgttgtca gcgtggtgct gggcgccatg gtggctctgg ctttgtggcg
      361 gttccgtttt ccgggcaaga cggccctgaa cggcgccctg gccctgccga tcgtggtgcc
      421 cgagatctgc atgggcgtgg ccatgctggt gttcttcgcg cgggtgatgc cgtggccgca
      481 gggcatggtc tggccgctga atctgggcgc gatcatcatc gcccacgtct cgttctcgtt
      541 ccccttcgtg gcggtggtgg tgcgggcgcg catgaccagc ttcaaccgcg agatggaaga
      601 ggccgcgcgc gaccttgggg ctggcgagtg gcggacgatc agggacgtga tcctgccgca
      661 catggccccg tcgctggtgg ccggcgctct gctggccttc acgctgagcc tggatgactt
      721 cgtcatcacc ttcttcacgg ccggacccga taccgtgacc ttcccggtca aggtctattc
      781 gatggtccgc ttctccgtga cgcccgaggt caatgcggcc tcgaccattc tgatcgtgct
      841 gaccgtgatc ctgacggctg tcgccctgaa gctgcagggc aattcggccg cgacggcggg
      901 tcatggggga gacaagtcgt gagcatcatc accttcgaga acgtcaccaa gcgctttggc
      961 aagatggtgg ccgtcgacaa cgtctcgctc agcgtcgagg agggcgagtt cttctcgctg
     1021 ctggggccgt cgggctgcgg caagaccacc ctactgcgga tgctggccgg gttcgagacg
     1081 ccgaccgagg gccgcatcct gatcgacggc caggatgtct cgaccgtgcc gcccaacaag
     1141 cggccggtga acatggtgtt ccagtcctat gcggtgttcc cgcacatgac cgtggccgac
     1201 aatgtcgcct atggcctgaa ggtcgaccgc gtgccgaaag ccgagcgcga ccggcgcgtc
     1261 gaggaggcgc tggagctggt ccaactgggc ggcctggggg ggcgcaagcc cgaccagctg
     1321 tcgggcggcc agcgccagcg tgtggccctg gcgcgcgcgc tggtcaaacg cccccgcgtg
     1381 ctgctgctgg acgagccgct gtcggccctg gacgccaagc tgcgcgagca gatgcgcacc
     1441 gagctgtgta ccctgcagga gaaggtcggc atcaccttta tcatggtcac ccacgaccag
     1501 gacgaggccc tggccctggc cagccgttgc gcggtgatga accggggcgt gctgcagcag
     1561 gtggccacgc ccagcgacct gtatgagttc cccaacagcc ggttcgtggc cgacttcatc
     1621 ggcagcgtga acctgttcga gggcgtgctg gcggtggacg agcccagcca cgcggtgatc
     1681 cgctcgcccg acctgccggt ggatatcttc ctggaccacg gcgtcactgg cccccgcggc
     1741 ggcgcggcct gggcggcgat ccggcccgag aagatcgagt tgcacaagaa ggacggcgac
     1801 gaggccccca atctcggcga cgcgcccaag ggcgcgaacg cggtagcggg cgtcatcaag
     1861 cacgaggcct atctgggcgg ctcatcggtc tatgaggtcg agatcgccgg aggccgtcgc
     1921 atcaaggtcc accgctccaa cctgacccgc tgggaccagg aagacttcaa gctgggcgaa
     1981 gaggtctggc tgtgctggca cgcctgttcg ccggcggtgt tgttgagctg atgcactctg
     2041 accttcccct ttctttcagc tgtcatcccg gacgagcgcg cagcgcgaag atccgggacc
     2101 gacgcgtgaa cgtctgcgct tccggcggtc ccggatctgc gcttcgcttg tccgggatga
     2161 cagccgaggg agcggggaag aaggcgctgc gcccatgacc cgccccgtcg ccggcatcat
     2221 ctgctgcacc cgcaccgtcg ggatcgagcc ggcccaggcg gtgatgaacc gctatgtcgc
     2281 cggcgcgatg cgccatgccg acgcggcggc tctgctggtt ccgtctatgc ctgaattgat
     2341 gaaggcgtcc gaggtcgccg cgcggctgga cggcctgatg ctgaccggca gtcnnnnnnn
     2401 nnngcgatgc gcccggcccg ttcgatctgg cccgcgacac catgaccgcc gacctgatca
     2461 aggccatgct cgacctgggc cggccggtgt tcggggtgtg ccggggcttt caggaaatca
     2521 atgtcgcgtt cggcgggacc ctgcgccgcg acatggcctc cagcgccgag ctgatcgccc
     2581 accacgcgcc agacgaggtc gacttcaacg gcatgtttga tcaccggcat ccggtcgacc
     2641 tgacgccggg cggggtgctg gctacggcgt ttggcgcatc gagcgcggtg gtcaattccg
     2701 tgcactatca gggggtagac cgcctgggcg aaggcctgac cgtcgaggcc cgggcacccg
     2761 acggcgtggt cgaggcggtg tcggccagcg ttaatggcgc gccggtcctg gccgtccaat
     2821 ggcaccccga gtggaagccc gaccagaacg tccagtccca gatcttcttc cagcttttcg
     2881 gacgcgccct cagaggcgcg ccgcttctcg tcgcgtagga aacacccatg accgtcccga
     2941 tccgcaacca cgatatcgcc gagctcaagc gcctggatct ggcccatcac ctgccggctc
     3001 aaaccgatta caagctgcag cacgacctgg gcggcagccg gatcatcacc cgcgctgacg
     3061 gggtctatat ccacgatggc gagggccatc agatcctcga cggcatggcc gggctgtggt
     3121 gcgtgaacgt gggctatggc cggcaggagc tggcccaggc ggcctatgag cagatgctgg
     3181 agctgcccta ctacaacacc ttcttcaaga cggcctcgcc gccgccgatc cggctggccg
     3241 ccaagatcgc cgagaagatg ggcggccacc tgacccacgt gttcttcaac tcgtcgggct
     3301 cggaggccaa tgacacggtc ttcnnnnnnn nnngaaaacc aagggccagc cgcagcgcac
     3361 cgccttcatc agccgctgga acgcctatca cggctcgacc gtggcggggg tgtcgctggg
     3421 cggcatgaag cacatgcatg tgcagggcga tctgccgatc cctggcgtgc atcacgtgat
     3481 gcagccctac agcttcaacg agggcttcga cgaagatccg gcggcgtttt gcgcgcgcgc
     3541 ggtcaaggag atcgaggaca agatccttga ggtcggcccc gagaccgtcg ccgcctttat
     3601 cggcgagccg gtgcaggggg cgggcggggt gatcatccct cccgacggat actggccggc
     3661 ggtcgaggcg gtgtgccgca agtacggcat cctgctggtc tgcgacgagg tgatctgcgg
     3721 cttcgggcgc ctgggccagt ggttcggcca ccagcactac gggatcaagc ccgacatgat
     3781 cgccatggcc aaggggctgt cgtcgggcta cctgccgatc agcgccgtcg gcgtggccga
     3841 cttcatcgtc gccgagttga aggagaaggg cggggatttc atccacggct tcacctattc
     3901 gggtcatccc accgcctgcg ccgtcgccct gaagaacatc gagattcttg agcgcgaggg
     3961 tctggtggag cgcac
//
LOCUS       DAOMEO010000151         3968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2696_length_3968_cov_2.652185, whole genome shotgun sequence.
ACCESSION   DAOMEO010000151 DAOMEO010000000
VERSION     DAOMEO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_3968_cov_2.652185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..208)
                     /locus_tag="PLH31_04685"
     CDS             complement(20..208)
                     /locus_tag="PLH31_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HQR88556.1"
                     /translation="MAARPALKRFGRNQSGATAVEVGLLVAGLCIAIISAVSMLSGGI
                     STSFTKTSTAMSTANSGS"
     gene            complement(187..591)
                     /gene="mutT"
                     /locus_tag="PLH31_04690"
     CDS             complement(187..591)
                     /gene="mutT"
                     /locus_tag="PLH31_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HQR88557.1"
                     /translation="MTKPTVLVAAAAMIDVDGRVLICQRPQGKQLAGLWEFPGGKVEL
                     GETPEECLIRELQEELGIKVAQSCLAPFVFASHSYESFHVLMPLYLLRRWEGLVTRKE
                     HEALAWVKPDKLADYPMPPADVPLVAWLRDLL"
     gene            693..1133
                     /locus_tag="PLH31_04695"
     CDS             693..1133
                     /locus_tag="PLH31_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR88558.1"
                     /translation="MYRPIATIAAGLALLIAGPALAQANPEEAGVRKALEHYLAGHAT
                     GDPKAFRQSFHDEARLQWIREGQYATRSDDEYVAGASGKPPADEAQRKRSIESIDITG
                     TAASAKIVLDYPTVVFTDYMHLLKTQDGWKIVNKTFHAQPKAKQ"
     assembly_gap    1384..1393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1485..2897
                     /locus_tag="PLH31_04700"
     CDS             1485..2897
                     /locus_tag="PLH31_04700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR88559.1"
                     /translation="MTQFSDLGLAKPLLKALTDKGYTTPTPIQAQAIPTVMTGRDLLG
                     IAQTGTGKTAAFALPILHRLAEDKKPAPRRGFRCLVLSPTRELATQIADSFRDYGKHM
                     GLTVATIFGGVKYGPQMKALAAGVDVVVATPGRLMDHLGEKSAHLNGVEIFVLDEADQ
                     MLDLGFVVPIRKIASQLPKERQNLFFSATMPNEIGKLAGELLKNPAQVSVTPQATTVE
                     RIQQSLIFIEAQRKRPLLAELLADSAVERSIVFTRTKRGADRVAKYLVASGIEAAAIH
                     GDKTQGQRERALAAFKAGQVKALIATDIAARGIDVNDVSHVFNFELPNVPESYVHRIG
                     RTARKGKEGIAISFCADDERTLLRDIQKATRQTIPTFDRRNDKSLAAAAAVADKLAPP
                     EKYVDPRRADPRNNVPAELRRQRNRPARGGDQAKSPGQGQGRGRNGPSNAAAKPAGGD
                     YRGPKRVSATAPAERWNPID"
     gene            3119..>3968
                     /locus_tag="PLH31_04705"
     CDS             3119..>3968
                     /locus_tag="PLH31_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR88560.1"
                     /translation="MAEVKPRVELALTRIAGGEMNERVRAFDWSRTPIGPMETWPPSL
                     RLAVDMILATNFPMAVRWGPELCLIYNDAYAPALHELHPAALGMRFSQHSDAFQASLR
                     TMHENILAGTSGGYALEKMALSIKRADDPTPRIGHFNISYSPLPDTTAPTGVGGVLIT
                     AVELTEAVETERTLRQTERRYRLAIEAAGGVGAWDWDIQADKVYADAGYAALHSLSPD
                     YVEAGLPVHSFTPAVHPEDRERIRQISLAAMETGGDFADEYRLVQSDGSIRWVYTRGS
                     CYLDADG"
BASE COUNT          732 a         1409 c         1230 g          587 t
ORIGIN      
        1 cggcgggcga aaagccggtt tagctgccgc tgttggccgt gctcatcgcg gtcgaggtct
       61 tggtgaagct ggtgctgatc ccgccgctca gcatcgagac ggcgctgatg atggcgatgc
      121 agaggcccgc gaccagcaga ccgacctcga cagcggtggc gccgctctgg ttgcgcccga
      181 aacgcttcag agcaggtcgc gcagccacgc cacgagcggg acgtccgccg gcggcatcgg
      241 atagtccgcg agtttgtccg gctttaccca tgccagcgcc tcatgctcct tgcgcgtgac
      301 caggccttcc cagcggcgca gcaagtagag tggcatcagg acgtgaaaag actcgtaact
      361 gtgggacgca aaaacaaacg gcgcgaggca ggattgagcg accttgatgc ccagttcttc
      421 ttgcagttcg cggatcaggc actcttccgg ggtctcgcca agctcgacct tgccgcccgg
      481 aaactcccac agccccgcca gttgcttgcc ctggggccgc tggcagatca gcacgcgacc
      541 atcgacatcg atcatggcgg cggcggcgac gaggacagtg ggcttggtca tgaagcgggt
      601 gtggcgtggg gaaacccggc tggcaagagc ttggccgcat ggccgccata ttgcctgcct
      661 agcttcacca aaacgggctg gagggaaccg ccatgtatcg accgatcgcg accatcgccg
      721 caggtctcgc cctgctgatc gccggcccgg ccctggccca ggccaatcct gaagaagctg
      781 gcgttcgcaa ggcgctggag cactatctgg cgggtcacgc gacgggcgac cccaaggcgt
      841 ttcgccagtc cttccacgac gaggcgcgac tgcaatggat ccgcgagggc cagtacgcca
      901 cgcgcagcga cgacgagtat gtcgccggcg ccagcggcaa gccgcccgcc gacgaggccc
      961 aacgcaagcg ctcgatcgaa tcgatcgaca tcaccggcac ggccgccagc gccaagatcg
     1021 tgctcgacta tcccaccgtc gtcttcaccg actacatgca cctgctgaag acgcaggacg
     1081 gctggaagat cgtcaacaag accttccatg cccaacccaa ggccaagcag tagatcctcc
     1141 ccttttgggg aggcgccggc gaagccgacg gagggggcaa gctggatgtc ggccgagctg
     1201 cccacctccg accctccggg ccacctcccc caaaagggga ggatcagggc cttgctcttg
     1261 acttcgcacc tgcaacaaac gatacggggt ttgcgccctt aggcgctatc ggtgctccag
     1321 ccgcgtgaag gtccctctag gaccagcctg tcgagcgccc tgaaacgtcc ataagaccgc
     1381 ccgnnnnnnn nnncgcgggg cgctccccaa ccgacccgcc tcatgggcga gccgcgcgag
     1441 gaccgcgcgc gccgtcatgc ggccttgtct gaaagacacc cgacttgact caattttctg
     1501 accttggcct ggccaagccg cttctcaagg ccctgactga caagggctat accaccccca
     1561 ccccgatcca ggcgcaggcc atccccacgg tcatgaccgg ccgcgacctg ctgggcatcg
     1621 cccagaccgg caccggcaag accgccgcct tcgccctgcc gatcctgcat cgcctggccg
     1681 aagacaagaa gccggctccg cgccgcggct tccgctgcct ggtcctgtcg ccgacccgcg
     1741 agctggccac ccagatcgcc gacagcttcc gtgactatgg caagcacatg ggcctgaccg
     1801 tcgccaccat cttcggcggc gtgaagtatg gcccgcagat gaaggccctg gcggccggcg
     1861 tcgacgtggt cgtggccact ccgggccgtc tgatggacca cctgggcgaa aagtcggctc
     1921 acctgaacgg cgtcgagatc ttcgtgctcg acgaagccga ccagatgctc gacctgggct
     1981 tcgtggtgcc gatccgcaag atcgccagcc agctgcccaa ggaacgccag aacctgttct
     2041 tctcggccac catgcccaac gaaatcggca agctggccgg cgagctgctg aagaacccgg
     2101 cccaggtgtc ggtcaccccg caagccacca ccgtcgaacg catccagcag tcgctgatct
     2161 tcatcgaggc ccagcgcaag cgccccctgc tggccgaact gctggccgac agcgccgtcg
     2221 aacgctcgat cgtcttcacc cgcaccaagc gcggcgccga ccgggtggcc aagtatctgg
     2281 tcgccagcgg catcgaagcg gccgccatcc acggcgacaa gacgcaaggc cagcgtgaac
     2341 gcgccctcgc cgccttcaag gccggccagg tcaaggcgct gatcgccacc gacatcgccg
     2401 cgcgcggcat cgacgtcaac gacgtcagcc acgtgttcaa cttcgaactg cccaacgtgc
     2461 cggaatccta cgtccaccgc atcggccgga ccgcccgcaa gggcaaggaa ggcatcgcca
     2521 tcagcttctg cgccgatgac gaacgcaccc tgctgcgcga catccagaag gctacccgcc
     2581 agacgatccc gaccttcgat cgccgcaacg acaagagcct cgccgccgcc gccgctgtcg
     2641 ccgacaagct ggccccgccg gaaaagtacg tcgatccgcg tcgcgccgac ccgcgcaaca
     2701 acgtgccggc cgaactgcgt cgccagcgca atcgccccgc gcgcggcggt gatcaggcca
     2761 agagcccggg ccagggccag ggtcgcggtc gcaatggccc ttcgaacgcc gcggccaagc
     2821 cggccggcgg cgactatcgc ggccccaagc gcgtcagcgc cacggcgccg gccgagcgct
     2881 ggaacccgat cgactgatcc ccaaaatggg tacgccgact tgaggcgcgc cctgtcatat
     2941 gactgaattg gcggccgttt tcatcccgag ggacgaaagc ggccgttttt ccgtccgtga
     3001 accggcccgg cgccccgggg tgggtctatc caggccagac aagccttgaa tcaagcttcg
     3061 cgatgggttc accatgtccg agggaccggt cactgaccgg tgggagggga cattcgcgat
     3121 ggcggaggta aagccacgcg ttgaactggc gctgaccagg atcgctggcg gcgagatgaa
     3181 cgagcgcgtc cgcgccttcg actggtcaag gacgcccatc ggcccgatgg aaacctggcc
     3241 gccaagcctg cgcctggccg tcgacatgat cctggccacc aacttcccca tggctgtacg
     3301 ctggggcccc gagctgtgcc tgatctataa cgacgcctac gcgcccgccc tgcacgagtt
     3361 gcaccccgca gcgctgggca tgcgcttcag ccagcactcc gacgcgtttc aggccagcct
     3421 gcggacgatg cacgagaaca tcctggctgg gaccagcggc ggctacgccc tcgaaaaaat
     3481 ggcgctgagc atcaagcgcg cggacgatcc cacgcccagg atcggacatt tcaacatcag
     3541 ctacagcccc ctgcccgata ccaccgcccc gaccggtgtc ggcggtgtgc tgatcaccgc
     3601 cgtggaactg accgaggcgg tcgagaccga acgcaccctg cgccagaccg agcggcgcta
     3661 tcgtctggcg atcgaggcgg ccggcggggt tggcgcctgg gactgggata tccaggccga
     3721 caaggtctat gccgacgccg gctatgcggc gctgcattcg ctgtcgcccg actatgtcga
     3781 ggcgggcctg ccggtgcaca gcttcacccc tgccgtccat cccgaagacc gcgagcggat
     3841 ccgccagatc agtctggcgg ccatggaaac cggcggcgac ttcgccgacg aatatcggct
     3901 ggttcagagt gatggctcga tccgctgggt ctatacgcgc ggcagttgct acctcgacgc
     3961 cgatggtc
//
LOCUS       DAOMEO010000152         3966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2697_length_3966_cov_2.737919, whole genome shotgun sequence.
ACCESSION   DAOMEO010000152 DAOMEO010000000
VERSION     DAOMEO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_3966_cov_2.737919"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PLH31_04710"
     CDS             <1..1430
                     /locus_tag="PLH31_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88561.1"
                     /translation="QTNTALPAPYTTVDDAYYAGAGIRDDQLYGLGLKMPLTDKIKLD
                     ATAYGHTNEGQGLWYTPYVASPGFGTAGSTAAPLSIRTTEYDISRSGLTAGLTIDLGA
                     HELTGGFWTEKNDMNQARRFYAETAAAPSRNSLQFQENPFFTQWQYRFETETLTGYVS
                     DTWTINDALKVNFGFKAMKVTNNVQRIAGNPLAGEIESENSFLPQVGVVYKVMPDLEF
                     FGGYTENMAAYVSAATSGPFASQNQAAVDYIAKTLNPETSKTIELGARYRTDRFQGVA
                     ALYHVTFEDRLLAASTAAPILGLPAVLSNVGGVETSGVELAGEYKLTDNWSLYGAYTY
                     NQSEYQDDVLNGSGVVVARTAGKEVVNTPKNIFKGEVKFELAGFYSKLGVAHTGERYY
                     TYENIGGQVDSTTVADLTLGYRFDGETWGKGLEIQANITNLTDEDYISTIGSGGFVNS
                     DPNGTTQTVLTAPPRQFFVSIKKTF"
     gene            1517..2629
                     /locus_tag="PLH31_04715"
     CDS             1517..2629
                     /locus_tag="PLH31_04715"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQR88562.1"
                     /translation="MTDLSRRSALGLSAAALAVAPAATAAVLDAPHPALARPLTRIAF
                     GSCAKQDKDQPIWEAIFDQKPDLFVFLGDNIYADTRDPKVMAAKYAMLAAKPHFKRLR
                     DTIPTVAIWDDHDFGENDAGVDYPMKAESRRQFCDFWGEPADSVRRTRDGVYAAYVFG
                     PAGQRVQVILPDLRWNRTPLAEKTFKGGYKAWATARGLSGQETPGPYDRIPDLNATMI
                     GEPQWTWLEQQLAQPADLRILGSSLQVLADFAGWESWINYARDHQRLLDAIRRQRADR
                     LICISGDTHYAEVSRLDVNVPYPLWDVTSSGLTEVWPVVPPNALRVSPVLRERNFGMI
                     EIDWSGAAPVVTLRVHDETGAVKISQVVDAGTLRVA"
     gene            2724..>3966
                     /locus_tag="PLH31_04720"
     CDS             2724..>3966
                     /locus_tag="PLH31_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQR88563.1"
                     /translation="MIRPSLTALAACLALSSPVLAEKLTPERIFADPSLSGPTARGVA
                     LSPDGKRVTYLKSKAEAANVQDLWAADVAGGEPYRLIDSASLSSGAKELSEAEKARRE
                     RARVFSRGIVEYSWDSQGRFILVPLDGDLYLDSVSDGKVSRLTETPGDEVDAKVSPLG
                     GYVSYVRDQNLYVKPVNGGAEKAVTTDGKDALSFGVAEFIAQEELDRFSGYWWSPDES
                     RIAYTRVDESGVDIVPRADIGPGGATVVLQRYPRAGRPNAVVDLFVQDLATGKAVQLD
                     LGANKDIYVARVDWSADGKTVYVQRLSRDQKTLDLIAFNPATGVGKTILTETDAHFIE
                     VHEDFRPLDDGSFLWSSEKSGFNHLYRHAADGKLIAQITKGDWPMNRVEGVDETRKVV
                     IFGASIDTPIERRLREVEAEGR"
BASE COUNT          722 a         1408 c         1213 g          623 t
ORIGIN      
        1 accagaccaa caccgccctg cccgcgccct acacgaccgt cgatgacgcc tattatgccg
       61 gcgccggcat ccgcgatgac cagctgtacg gcctgggcct gaaaatgccc ctgaccgaca
      121 agatcaagct cgacgcgacc gcttacggcc acaccaacga aggccagggc ctctggtata
      181 cgccctatgt ggccagcccg ggcttcggca cggccggttc caccgccgct cccctgtcga
      241 tccgcaccac ggaatacgac atcagccgct cgggtctgac cgccggcctg accatcgatc
      301 tgggcgccca cgagctgacc ggcggcttct ggaccgaaaa gaacgacatg aaccaggcgc
      361 gccgcttcta tgccgaaacc gcggcggccc cgtcgcgcaa ctcgctccag ttccaggaaa
      421 acccgttctt cacccagtgg cagtaccgct tcgagaccga aaccctgacc ggctatgtct
      481 ccgacacctg gacgatcaat gacgccctga aggtcaattt cggcttcaag gccatgaagg
      541 tgacgaacaa cgtccagcgc atcgcgggca acccgctggc cggcgagatc gagtcggaaa
      601 actcgttcct cccgcaggtc ggcgtcgtct acaaggtcat gccggacctg gagttcttcg
      661 gcggctacac cgagaacatg gccgcctacg tctcggccgc cacctcgggc ccgttcgcct
      721 cgcagaacca ggcggcggtg gactacatcg ccaagaccct gaaccctgaa acctccaaga
      781 ccatcgaact gggcgcgcgg tatcgcaccg atcggttcca gggcgtcgcg gccctctatc
      841 acgtgacctt cgaggaccgc ctgctggccg cctcgacggc cgcgccgatc ctgggcctgc
      901 cggccgtgct gtccaatgtc ggcggcgtgg aaaccagcgg cgtcgaactg gccggtgaat
      961 acaagctgac cgacaactgg tcgctctacg gcgcctacac ctacaaccag tcggaatatc
     1021 aggacgacgt tctgaacggc tcgggcgtgg tcgtcgcccg caccgccggc aaggaagtcg
     1081 tcaacacgcc caagaacatc ttcaagggcg aagtgaagtt cgaactggcc ggcttctatt
     1141 cgaagctggg cgtcgcccac accggcgagc gctactacac ctacgagaac atcggcggtc
     1201 aggtggacag caccaccgtc gccgacctga cgctgggcta tcgctttgac ggcgagacct
     1261 ggggcaaggg tctggaaatc caggccaaca tcaccaacct gaccgacgag gactacatct
     1321 cgaccatcgg ttcgggcggg ttcgtcaaca gcgacccgaa cggcacgacc cagacggtgc
     1381 tgaccgcgcc gccgcgtcag ttcttcgtct cgatcaagaa gaccttctaa gcccttcgcg
     1441 atcgtaaaac ctggggccgg gaggcgactc ccggcccttt gtcgttcaag ctgcgccgcc
     1501 acacgaggaa acgtccgtga ccgatctctc ccgtcgctcg gctctgggcc tgtcggccgc
     1561 cgccctggcc gtcgccccgg ccgccacagc cgccgttctc gatgcgccgc acccggcgct
     1621 ggccagaccc ctgacccgca tcgccttcgg ctcctgcgcc aagcaggaca aggaccagcc
     1681 gatctgggag gcgatcttcg atcagaagcc tgacctgttc gtgttcctgg gcgacaacat
     1741 ctatgccgac acccgcgatc ccaaggtgat ggcggccaaa tacgccatgc tggccgccaa
     1801 gccgcacttc aagcgcctgc gcgatacgat ccccacggtc gccatctggg acgatcacga
     1861 ctttggcgaa aacgacgccg gggtcgacta tccgatgaag gccgagagcc gccggcagtt
     1921 ctgcgacttc tggggcgagc ccgccgattc ggtgcgccgc acccgcgacg gggtctatgc
     1981 ggcctatgtg ttcggccccg ccggccagcg ggtgcaggtg atcctgcccg acctgcgctg
     2041 gaaccgcacg cccctggccg aaaagacctt caagggcggc tacaaggcct gggccacggc
     2101 gcgcggcctg tctggccagg aaacgccagg gccctatgac cgcatccccg acctgaacgc
     2161 caccatgatc ggcgagccgc aatggacctg gctggagcag cagctggctc agcccgccga
     2221 cctgcgcatc ctgggctcca gcctgcaggt gctggccgat ttcgccggct gggaaagctg
     2281 gatcaactat gcccgcgacc accagcgcct gctggacgcc atccgccgcc aacgcgccga
     2341 ccgcctgatc tgcatcagcg gcgacaccca ctatgccgag gtctcgcggc tggacgtcaa
     2401 tgtcccctac ccgctgtggg acgtcacctc ctcgggcctg accgaggtgt ggccggtggt
     2461 cccgcccaac gccctgcggg tctcgccggt gctgcgcgag cgcaatttcg ggatgatcga
     2521 gatcgactgg agcggggcgg cgccggtcgt gacactgcgc gtccatgacg agacgggcgc
     2581 ggtgaagatt tcgcaggtgg tggatgcggg gacgttgcgg gtggcctgac ccaagtcctc
     2641 ccccttcagg gggaggacca accgccaccc atcaaacttt taactgctga caacggcaaa
     2701 gcctcgcact agcgtcccgc gccatgatca gaccctccct taccgccctc gcggcgtgcc
     2761 tcgccctgtc gtcgccggtt ctggcggaaa agctcacccc cgaacggatc ttcgccgatc
     2821 caagcctgtc gggccccacc gccaggggcg tggccctgtc gccggatggc aagcgcgtta
     2881 cctatctgaa gtccaaggcc gaggccgcca acgtccagga cctgtgggcc gccgacgtgg
     2941 ccggcggcga gccctatcgc ctgatcgact cggcgagcct gtcgtcgggc gccaaggagc
     3001 tctcggaagc cgagaaggcc cgccgcgagc gcgcccgcgt cttctcgcgc ggcatcgtcg
     3061 aatacagctg ggacagccag ggccgcttca tcctggtccc gctggatggc gacctctatc
     3121 tggacagcgt ttcggacggc aaggtcagcc gcctcaccga aaccccgggt gatgaagtcg
     3181 acgccaaggt ctcgccgctg ggcggctatg tctcctatgt ccgcgaccag aacctctatg
     3241 tgaagcccgt caatggcggg gccgaaaagg ccgtgaccac cgacggcaag gacgccctgt
     3301 cgttcggcgt cgccgagttc atcgcccagg aagagctgga ccgcttcagc ggctattggt
     3361 ggtcgcccga cgagagccgc atcgcctata cgcgcgtcga tgagagcggc gtcgacatcg
     3421 tgccgcgcgc cgatatcggt ccgggcggcg ccacagtagt gttgcagcgc tatccccgcg
     3481 ccggccgccc caacgccgtg gtcgacctgt tcgtgcagga cctcgccaca ggcaaggcgg
     3541 tgcagctgga cttgggcgcc aacaaggaca tctatgtcgc ccgtgtcgac tggtcggccg
     3601 atggcaagac ggtctatgtg cagcgcctga gccgtgatca gaagaccctg gacctgatcg
     3661 ccttcaaccc cgccaccggc gtgggcaaga cgatcctgac cgagaccgac gctcatttca
     3721 ttgaggttca cgaagacttc cgcccgctgg acgatggctc gttcctgtgg tcgtcggaaa
     3781 agtccggctt caaccacctc tatcgccacg ccgccgacgg caagctgatc gcccagatca
     3841 ccaagggcga ctggcccatg aaccgggtcg agggcgtcga cgagacgcgc aaggtcgtga
     3901 tcttcggcgc ctccatcgac accccgatcg agcgccgcct gcgcgaggtc gaagcggaag
     3961 ggcgca
//
LOCUS       DAOMEO010000153         3958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2699_length_3958_cov_2.860364, whole genome shotgun sequence.
ACCESSION   DAOMEO010000153 DAOMEO010000000
VERSION     DAOMEO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_3958_cov_2.860364"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..759
                     /locus_tag="PLH31_04725"
     CDS             193..759
                     /locus_tag="PLH31_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQR88564.1"
                     /translation="MTILVLGLVLFLGVHSVRIFAGSFRDAQVAVSPGRWKGLYSLAS
                     AIGLGLIIWGWMQYRLMAPQLYDPPAWGRHASMALMLPAFILLAGSNGPVGRIKATVR
                     HPMLIGIILWSVAHLMANGDQASALLFGAFLAYGLVDLVSALGRREPPPVATKPMADV
                     IMVVAGTALYALFVLFLHRVFFGVSPLG"
     gene            complement(763..1449)
                     /locus_tag="PLH31_04730"
     CDS             complement(763..1449)
                     /locus_tag="PLH31_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQR88565.1"
                     /translation="MTLDWKRIAAELDARGWALTGPMFTPEQCATLAALYDQPALFRS
                     RVVMARHGYGKGEYQYFAYPLPEPIQSLRMSLYPPLAWIANRWAERLGEERRFPETLA
                     EMLERCHAGGQARPTPLMLTYGPGDYNCLHQDLYGEHVFPLQAAFLLDAPGRDFEGGE
                     FVLVEQRPRQQSAPQVVPLGQGEGVIFAVRERPAEGSRGVHRRMLRHGVSEVRSGRRR
                     TLGVIFHDAT"
     gene            complement(1446..1688)
                     /locus_tag="PLH31_04735"
     CDS             complement(1446..1688)
                     /locus_tag="PLH31_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HQR88566.1"
                     /translation="MNPSAYPDDDARWAACLARDPAADGAFFIAVRTTKIYCRPVCPA
                     RPLRKNVVFHATGAQARAAGFRACKRCKPDLLETAA"
     gene            complement(1771..>2130)
                     /locus_tag="PLH31_04740"
     CDS             complement(1771..>2130)
                     /locus_tag="PLH31_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HQR88567.1"
                     /translation="NDLDLDFQDLARADVKFLKVGAQMMLDQLQEQDGKLVIASLPDL
                     NASDFAALTRRYGIEVIVEKVEAEKQIAEILDLDIGYGQGHLFGEPRAIRDAVLAEAE
                     PPVDFMRGALRRRASGW"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2141..3043)
                     /locus_tag="PLH31_04745"
     CDS             complement(<2141..3043)
                     /locus_tag="PLH31_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172270651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR88568.1"
                     /translation="MRRLMLGLLTGAYLCLALIISLVLWRTGAAPAVGLSAFIGTLGL
                     CFAFHGLISNTFDSARTRLDIDTVREAHAILLDQIEKIDARMTQLVESVALDAQRRSE
                     ELSTEVHQLEDLIAGMNERLEHQLTHQVAAARVEVRGRTPQSNVMLQVVQEALAENRV
                     DLYLQPIVSLPQRRTVFYESFSRLRDDTGRVMMPAEYLAVAEPEGLMTAIDNLLLFRC
                     VQIVRRLAKQDRKVGIFCNISLASLADESFFPQFLEFMQANKDLAGALIFELGQAAFE
                     RRGAVEARHMSRLASLGFSFSLDKV"
     gene            3236..3553
                     /locus_tag="PLH31_04750"
     CDS             3236..3553
                     /locus_tag="PLH31_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQR88569.1"
                     /translation="MSDALAFDSSTDLASLGRSLGEASRGDAGVIVSVTGQTGAAEAV
                     EADELERRLLEMGFVEGAHVEVLHEGLFGRDPIAVRLDDTRVALRRREASAVTVKFDR
                     TPT"
     gene            3550..>3958
                     /locus_tag="PLH31_04755"
     CDS             3550..>3958
                     /locus_tag="PLH31_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HQR88570.1"
                     /translation="MSTEAATLGPARIALVGNPNSGKTALFNALTGSRQKVANYAGVT
                     VERKEGALTTAGGRTLRILDLPGTYSLRARSPDEVVTRDAILGRLAGEPAPDVLVCVA
                     DATNLRLVLRLVLELKQVGRPFVLALNMYDIAQR"
BASE COUNT          623 a         1283 c         1362 g          680 t
ORIGIN      
        1 atgtcgtagc ggtcggacag ggcggaaagg ccggcggggt aggtgatcat ggctgggcgc
       61 gtaccggtcg ggcggctggg cgtcaacttg acggacggcc tgggtgacgc ctggccgtgc
      121 ggtttcaagg ggcgtccctt ccggtgaacg ccgtttcgtg actaccctgc tcacgaagac
      181 ggggagagac agatgacgat tctggtcctg ggacttgtgc tgtttttggg cgttcactcc
      241 gtgcgaattt tcgccgggtc gtttcgggac gcgcaggtcg cggtcagtcc tggccgctgg
      301 aagggactct attccctggc ttcggccatc gggcttggcc tgatcatctg gggctggatg
      361 cagtatcggc tgatggcccc gcagctttat gatccgccag cctggggccg tcatgcttcc
      421 atggccctga tgctgccggc cttcatcctg ctggccggct cgaacggtcc cgttggccgg
      481 atcaaggcga cggtgcgcca tccgatgctg atcgggatca tcctgtggtc ggtggcgcat
      541 ctgatggcca atggcgacca ggcctcggcg ctgctgttcg gcgcgtttct ggcctatggc
      601 ctcgtcgatc tggtctcggc gttgggacgc cgggaaccgc cgcccgtggc gaccaagccc
      661 atggccgacg tgatcatggt cgtggccggg acagcgctct acgcgctgtt cgtgctgttc
      721 ctgcaccggg tgttcttcgg cgtgtcgccg ctgggatagc cctcaggtgg cgtcgtggaa
      781 gatcaccccc agcgtgcgtc gccgcccgct gcgaacctcg ctgactccat gccgcagcat
      841 gcgccgatgg acgccgcgcg acccttctgc cggtcgttca cgcaccgcga agatcacgcc
      901 ttcgccctgg cccaggggca cgacctgggg cgctgactgc tggcgcggcc gctgctcgac
      961 cagcacgaac tcgccaccct cgaaatcgcg ccccggcgcg tcgagcagaa aggccgcctg
     1021 cagcgggaag acatgctcgc catacaggtc ctggtgcagg cagttgtagt cgcccggacc
     1081 ataggtcagc atcagcggcg tcggccgggc ctggccgccc gcgtggcagc gctccagcat
     1141 ttccgccagg gtttctggaa agcgccgctc ctcgcccagc cgctcggccc agcgattggc
     1201 gatccaggcc aggggcggat agaggctcat ccgcaggctc tggatgggct cgggcagcgg
     1261 ataggcgaaa tactggtact cgcccttgcc atagccgtgc cgggccatga ccacgcgact
     1321 gcgaaacagc gccggctgat catacagcgc cgccagcgtc gcacattgct cgggggtgaa
     1381 catcggaccc gtcagggccc agccgcgggc gtccagctcg gcggcgatgc gcttccagtc
     1441 cagggtcaag cggcggtctc gagaaggtcg ggcttgcagc gcttgcacgc ccggaagccg
     1501 gcggcgcggg cttgcgcgcc ggtggcgtga aacaccacgt tcttgcgcag cggacgcgct
     1561 ggacagaccg ggcggcaata gatctttgtg gtgcgcacgg cgatgaagaa cgcgccgtcg
     1621 gcggcgggat ctcgggccag gcacgcggcc cagcgggcgt cgtcgtcagg ataggcggat
     1681 gggttcatgg cgggggctcc cttcgacggg agcctgcgcc ttcatcgcct gtcggtcatt
     1741 ccgaagcttg cggccgggta ccgaaaactg tcaccagcct gaagcgcgac gacgcagggc
     1801 gccgcgcatg aagtcgaccg gaggctcggc ctcggccagc acggcgtcgc ggatggcgcg
     1861 gggctcgccg aacaggtgac cttggccata gccgatgtcg agatccagga tctcggcgat
     1921 ctgcttttcg gcctcgacct tctcgacgat cacctcgatg ccgtagcggc gggtgagggc
     1981 ggcgaagtcg ctggcgttca ggtcgggcag cgaggcgatc accagcttgc cgtcctgctc
     2041 ctgcagctgg tccagcatca tctgggcgcc gaccttgagg aacttgacgt cggcgcgggc
     2101 caggtcctgg aagtccaggt ccaggtcatt nnnnnnnnnn gaccttgtcg aggctgaagc
     2161 tgaagcccag gctggccagg cgcgacatgt ggcgggcttc caccgcgccg cgccgctcga
     2221 aggcggcctg gcccagttcg aagatcagcg cgccggccag gtccttgttg gcctgcatga
     2281 attccaggaa ctgcgggaag aagctctcat cggccaggct ggccagcgag atgttgcaga
     2341 agatcccgac cttgcggtcc tgcttggcca gccgccgcac gatctgcacg cagcggaaca
     2401 gaagcaggtt gtcgatggcg gtcatcaggc cttcgggctc ggccacggcc agatattcag
     2461 ccggcatcat cacccggccg gtgtcgtcgc gcaggcgcga gaagctttcg tagaagacgg
     2521 tccggcgctg gggcaggctg acgatgggct gcagatagag gtcgacccgg ttttcggcca
     2581 gggcctcctg caccacctgc agcatgacat tggattgcgg ggtgcggccg cgaacctcga
     2641 cgcgggcggc ggcgacctga tgggtcagct ggtgctccag ccgctcgttc atgccggcga
     2701 tcaggtcttc cagctggtgg acctcggtcg acagttcttc cgaacgacgt tgggcgtcca
     2761 gggcgacgct ctcgaccagc tgggtcatgc gggcgtcgat cttctcgatc tggtccagca
     2821 ggatggcgtg ggcctcgcgc acggtgtcga tgtccaggcg cgtacgagcg gaatcgaagg
     2881 tgttggagat cagaccgtga aacgcgaagc acaggcccag cgtgccgata aaggccgaca
     2941 gaccgacagc cggagccgcc cccgtgcgcc acagcaccag ggagatgatc agagccagac
     3001 aaagataggc gcccgtgagc agccccagca tcagccttcg catcagtccg tccacgcgaa
     3061 tcgttacagg caatcagacc ggtccagacc ccaagagtcg aacagattaa cgcgcctgcg
     3121 ccattccgcc tcgccccttg ggagtcccgg acagcataac gcaacccggt gagaacgccg
     3181 catcgacagc agggctttgg actgataatc actcgcaagt tttcgcggag cggccatgtc
     3241 tgacgccctt gcctttgatt ccagcactga tcttgcctct ctcgggcgct cgctcggtga
     3301 ggcttcgcgc ggtgatgcgg gcgtgatcgt cagcgttacc ggccagaccg gcgcggccga
     3361 agccgtcgag gccgacgagc tggagcgacg cctgctggag atgggcttcg tcgagggcgc
     3421 gcatgtcgag gttctgcacg agggcctgtt cggtcgtgat ccgatcgccg tgcggctgga
     3481 cgatacccgc gtggccctgc gtcgacgcga ggccagcgcc gtgaccgtga aattcgacag
     3541 gaccccgact tgagtactga agccgctacg ctcggccccg cgcgcatcgc cctggtcggc
     3601 aatcccaatt ccggcaagac ggccctgttc aacgccctga ccggcagccg ccagaaggtg
     3661 gccaactatg ccggcgtcac cgttgagcgg aaggaaggcg cgctgaccac ggccggtggc
     3721 cggaccctgc gcatcctcga cctgcccggc acctattccc tgcgcgcccg cagtcccgac
     3781 gaggtggtga cccgcgacgc catcctgggc cggctggccg gggagcccgc gcccgacgtg
     3841 ctggtctgcg tggccgacgc caccaatctg cggctggtgc tgcgcctggt gctggagctg
     3901 aaacaggtgg gccggccgtt cgtgctggcg ctgaacatgt acgatatcgc ccagcgcc
//
LOCUS       DAOMEO010000154         3958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2700_length_3958_cov_2.399693, whole genome shotgun sequence.
ACCESSION   DAOMEO010000154 DAOMEO010000000
VERSION     DAOMEO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_3958_cov_2.399693"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLH31_04760"
     CDS             complement(<1..518)
                     /locus_tag="PLH31_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR88571.1"
                     /translation="MATDPTAVQLANELADLIKRSGLKSGAVVASEQDLRARHDVGRS
                     VLRQAARILEERGVAFMKRGAGGGLIVAPPDPEAAGRALAILLESRMQGPHALDRLLK
                     ATDTHVFLTATPRLDLIECERVRQLAIRLRRQTEAGLRPLGEIRQLLSAIRRLVKDPL
                     AGLAYQTTAEYG"
     gene            complement(706..>2185)
                     /locus_tag="PLH31_04765"
     CDS             complement(706..>2185)
                     /locus_tag="PLH31_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88572.1"
                     /translation="NEPVYAQLQAALAAQNARGPRSIATNYEPDFIKLDSQGWINTTN
                     WLINEHLTLKNIYSTRRFTAGQSYDLDGSPVTILHVTNPVGPKSKSTSEEVQAQFNFG
                     NINGVLGYFTEDTKTPLIYGFDTRQFVRIPGLLPNGGVIQAVNIAQSTSGSEAYFGQV
                     DWAVTDRFTLTGGVRKTTDERSSGPSETFVLFSPLQPYPTGLSAAAGKRLFVSFEATT
                     WNVAANYQVSHDLNVYATVRKGFKSGGFNGTAINPADQFFKPEEVTDYEAGVKGLANL
                     SGWQVRYAIDAFYDDYTNIQRFVNLATVPASTVTRNAATGSIKGVDLDVTVAQTDWLS
                     ASVQYTYLDAKYDSYNDALLGDLSNSRFPNTPKHQLNFTPRVEFPMPEGMGELSALAS
                     IYHQTGIATDPANVPNGNALVALSSAGTNIEGYTRVDLRADWRNIKGTGVSAAAFVRN
                     ATNKDYITGTNNQLTSQFGVATYLYGQPRFIGVELRFDLGTR"
     assembly_gap    2186..2195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2275..2859)
                     /locus_tag="PLH31_04770"
     CDS             complement(2275..2859)
                     /locus_tag="PLH31_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR88573.1"
                     /translation="MLDDIVVTARRREERLQEVPVAVTALSEEVLEEKGVRDANDLGQ
                     VAPGLSVQNTTANRNNITYSIRGQGQAFGQNSPGVVPYFAEVPSFGDAIFDLQGIQVL
                     KGPQGTLFGRNTTGGAILFTPRAPSEELNGYVLGRVGNYNRRASSLAWAVPSWATSSC
                     SASLARCCVAMAIPKMSRPATRWTTSTAKPGAPA"
     gene            3295..>3958
                     /locus_tag="PLH31_04775"
     CDS             3295..>3958
                     /locus_tag="PLH31_04775"
                     /inference="COORDINATES: protein motif:HMM:NF019157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HQR88574.1"
                     /translation="MLDVADKKALDTEGYAVRDSYFGAPYIDRDEWREVPYPHRNVHG
                     GFANTDTRFTFYFPAQEEWGGRMYHPLEGAHAGHEEAFAGAMGALLGGLDMMVKLGGY
                     MVESNSGHIGDDIDPRAGIDPGLYGYRASVESARFSKYICKQVFGREPDYSYVWGGSG
                     GGRRSPLCLEYCDGVYDGALPFMGGGNVEPHGTNSRVRSEQPFSFGSMFNVQRLLRGD
                     KLD"
BASE COUNT          698 a         1198 c         1282 g          770 t
ORIGIN      
        1 tctccatact cggcggtcgt ctggtaggcg aggcccgcaa gcgggtcttt caccaagcgt
       61 cggatggcgc tcagcagttg ccggatttcg cccagaggcc gcaggcccgc ctcggtctgg
      121 cgcctcagtc gaatggccag ttgccggacc cgttcgcact cgatcaggtc gagccgtggg
      181 gtcgccgtca gaaagacgtg ggtgtctgtc gctttcaata gccggtccag cgcatggggt
      241 ccctgcattc ggctttccag caggatggcg agcgctcgcc ccgccgcttc aggatcggga
      301 ggcgcgacga tcaggccgcc cccggccccg cgcttcatga aggcgacgcc ccgctcctcg
      361 aggatgcggg cggcttgccg aagcaccgag cggccgacgt cgtggcgcgc gcgcaggtcc
      421 tgttccgatg cgaccacggc ccccgacttg aggcccgagc gcttgatcag atcggcgagt
      481 tcgttggcga gttgaactgc ggtggggtcg gtagccatta ttcgtttccg tccgcggtct
      541 ctaaaccacg gctcccggcc ctattgatcg ggacaccttg atcccgccga acgcaaggcg
      601 tccgggtatc cgaagcaaag cgaacgccgc cgatcgatat ccgatccggg aaaacccgaa
      661 cagcgaatgt cgatcgacgg cgtcggccag attaggcgag ctgtgttagc gggttccgag
      721 gtcgaagcgc agttcgacac cgataaagcg cggttgccca tacagatagg tggcgacccc
      781 gaactggctg gtcagctggt tgttggtgcc ggtaatatag tccttgttgg tggcgttgcg
      841 gacaaaggcg gcggcgctga cgccggtgcc cttgatgtta cgccagtcgg cccgcagatc
      901 gacccgggta tagccttcga tattggtccc cgcgctggac agggcgacca gcgcattgcc
      961 gttggggaca ttggccggat cggtcgcgat accggtctga tggtagatgc tggccaaggc
     1021 cgacagctcg cccatgccct ctggcatcgg gaactccacc cgaggggtga agttcagctg
     1081 atgcttcggc gtgttcggga accggctatt ggacagatcc ccaagcagag cgtcgttgta
     1141 gctgtcatat ttggcgtcca gataggtgta ttgcaccgag gcgctaagcc agtccgtctg
     1201 cgcgaccgtc acgtccagat cgaccccctt gatagaaccg gtggcggcgt ttcgggtcac
     1261 cgtcgaggcc ggcaccgtgg ccaggttcac gaaacgctga atgttggtgt agtcgtcata
     1321 gaaagcgtcg atcgcatagc gcacctgcca accgctcaga ttggccagac ccttcacccc
     1381 cgcttcgtag tcggtgacct cctcgggctt gaagaactgg tccgccgggt tgatcgccgt
     1441 gccgttgaag cccccgctct tgaagccctt gcgaacggtc gcatagacgt tgagatcgtg
     1501 gctgacctga tagttcgcgg ccacgttcca ggtcgtggct tcgaacgaaa cgaacaatct
     1561 cttgcccgcc gcggcgctca agccggtcgg atagggctga agcggcgaaa acagcacgaa
     1621 ggtctcggac ggtccgctgg aacgctcgtc cgtggtcttg cggacgccgc ccgtcagggt
     1681 gaaccgatcg gtgacggccc agtcgacctg cccgaagtac gcttcactac ccgacgtact
     1741 ctgggcgata ttgacggcct ggatgacgcc gccgttgggc agcaagccgg ggatgcgaac
     1801 gaactgacgc gtgtcgaagc cgtagatgag cggggtcttc gtatcctcgg tgaagtatcc
     1861 gaggacgccg ttgatgttcc cgaagttgaa ctgggcttgg acttcctcgc tggtcgactt
     1921 ggatttcgga cccaccggat tggtgacgtg caggatcgtg accgggctgc cgtccaggtc
     1981 ataggactgg ccggccgtga agcggcgcgt gctgtagata ttcttcagcg tcagatgttc
     2041 attgatcagc cagttggtgg tgttgatcca tccttggctg tcgagcttga tgaaatcggg
     2101 ctcgtagttg gtggcgatgg accgggggcc gcgagcgttc tgggccgcca gggccgcctg
     2161 caattgggca tagaccggct cgttcnnnnn nnnnnaacag tcccgagccg gcctcatcga
     2221 cgttgacgtc ctgatacagc agggtgttct cgaagccttc gaacggacgg gccgtcaggc
     2281 tggcgcgcca ggcttggcgg tgctcgtcgt ccatcttgtc gccggtcgcg acatttttgg
     2341 tatagccatc gcgacgcagc acctggccag cgacgcggaa catgagcttg tcgcccagga
     2401 tgggaccgcc caggccaaac tcgacgcccg acgattgtaa ttgccaaccc gtcccagcac
     2461 gtaaccgttg agctcttcgc tcggcgcgcg gggcgtgaat aggatcgcgc cgccggtggt
     2521 gttacgacca aatagcgtac cctgcggccc cttcagaacc tggatgcctt gcaggtcgaa
     2581 gatggcgtcc ccgaacgagg ggacttcggc gaaataggga acgacgccgg gcgagttctg
     2641 gccaaacgcc tgcccctgac cccggatcga ataggtgatg ttgttgcggt tggccgtggt
     2701 gttctgaacg ctgaggcccg gcgcgacctg acccaggtcg ttggcgtccc ggacaccttt
     2761 ttcctcgagt acttcttcgc tcagcgcggt gacggcgacg ggcacttcct gcagccgctc
     2821 ctcgcggcgc cgggccgtga ccacgatgtc gtcaagcacg aacgcctgat ttgggtcact
     2881 cgatggcggg gtcgcttgtt gcgccaacgc gaatgccggc gccgttaggc aggtcgcgaa
     2941 catcaggccg gcaaggccga gcttgcgaat tgggcgcgct gaattggcgc cagaagcgtg
     3001 tccgttcatc gtttccctcc gtgccggccc cgccctttgt ggacgttggt ggaatgtttt
     3061 ccccgccatg cattgaacaa atcagtctca catgttcaga atatcggcaa ggacattcgt
     3121 ctgaagtcag aatgcctaca gcagcgcttg gatttgaact cggccgatga ggtcacccat
     3181 ttcgagatat tcccaacaga gaatggttga ctgatttcag aacggccgca tagattggga
     3241 tcaccggtcg gagagccggg gccgaaaccg gtcacacatt gagagggacg ccgaatgctg
     3301 gacgtggccg acaagaaggc gctggacacc gagggctacg ccgttcggga cagctatttt
     3361 ggggcgccct acatcgacag ggacgagtgg cgcgaagtcc cctatcccca tcgcaatgtg
     3421 catggcgggt tcgccaacac cgacacccgc ttcaccttct attttcccgc ccaggaagag
     3481 tggggcgggc gcatgtacca cccactggag ggcgcgcatg cgggccacga ggaagccttc
     3541 gccggtgcga tgggcgccct gctcggcggc ctcgacatga tggtcaagct cggcggctat
     3601 atggtggagt caaactcggg ccacatcggc gatgacatcg acccccgcgc tggtatcgat
     3661 ccgggcctct acggctatcg cgccagcgtc gagagcgcgc gcttctccaa atatatctgc
     3721 aagcaggtgt tcggccgcga accggactac agctatgtgt ggggcggcag cggcggtggg
     3781 cggcgatcgc ctctgtgcct cgaatattgc gacggcgtct atgacggcgc cctgcccttc
     3841 atgggcggcg gcaatgtcga accgcacggc accaactcgc gcgtccgcag cgaacagccc
     3901 ttctcgttcg gcagcatgtt caacgtccag cggcttctgc gtggcgacaa gttggacg
//
LOCUS       DAOMEO010000155         3948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2705_length_3948_cov_2.656306, whole genome shotgun sequence.
ACCESSION   DAOMEO010000155 DAOMEO010000000
VERSION     DAOMEO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2705_length_3948_cov_2.656306"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLH31_04780"
     CDS             <1..477
                     /locus_tag="PLH31_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR88575.1"
                     /translation="DASVTLGVRYTFGAPPPPPPPPPAPPAPPPAPPAPPAPPPPPPA
                     AVYEAREFVVYFPFDQYVLTPEAQAVVQQAADYAKAGMATKLTITGHTDTSGSDRYNA
                     RLSERRAKAVADGLVGMGVAATAVAVDWKGESAPAVATGDGVKEPLNRRSTISINF"
     gene            complement(751..2535)
                     /gene="mnmD"
                     /locus_tag="PLH31_04785"
     CDS             complement(751..2535)
                     /gene="mnmD"
                     /locus_tag="PLH31_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HQR88576.1"
                     /translation="MTDASPDDASPLVWNEDGLPRSRLYGDVYFSSADGLAETRAVFL
                     TGCGLPERFAGRDRLVVGELGFGTGLNIAALLDLWRREAPAQARLHIFSIEAHPISRE
                     DAARALSVWPELGEAAQALLDHWPGQARGFHRVDLPGFRATLDLAVTDVRQALESWDG
                     AADAWFLDGFSPALNPAMWSEDILAAVAARSAPGARAATFTVAGAVRRGLQAAGFEIA
                     KRPGFGRKKDRLEAWLPDAPSASPSPARLAIIGGGIAAAALARAARAQGLEVSVIDAD
                     DAPASGNPAALVTPALDAGGGPRAALPAQAFARAVDLYEALPGAVIARGVLQLAQSQP
                     AEERFAVVAGQDIFEPGTMTLLTSAADPLGEPAGAALVMSRALVIEPRGVLDAWRGET
                     LAARVAGLERHADAWRLLDGNGGEILRADAVIIAAGAGLGDLWPTLGYRPVRGQASWT
                     AVAEPGCTPAAFGGYAIPTRDGVLFGATHDRGVLDTDVRSEDHRRNLEILAKGLPQLA
                     ERLGRVPLSGRAATRVATPDHLPLAGQLAEGLFVLSGLGGRGFCLAPLLAEHVVARVT
                     GAPSPLPRELSRLLMPDRFDSPVARAGV"
     gene            complement(2633..2959)
                     /locus_tag="PLH31_04790"
     CDS             complement(2633..2959)
                     /locus_tag="PLH31_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88577.1"
                     /translation="MNTTVLRTLLIAGVAVSALSLAACGKKEEGADASTAAAEAAAAG
                     ASTVTDAAAAHDQAVADANKAAADAAAAPMAEGDDAATTATAEAPASTAKEPAQPAAA
                     PEKPAH"
     gene            complement(3123..>3948)
                     /gene="motA"
                     /locus_tag="PLH31_04795"
     CDS             complement(3123..>3948)
                     /gene="motA"
                     /locus_tag="PLH31_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HQR88578.1"
                     /translation="GSFIMAGGKMDVIIHAAPHELMAILGAGVAAFLIANSMTVIKAT
                     AAGFGKIFAGPKWKNSDYRDLLALLFLLTKTMKSKGVIALESHIEKPHESSIFSRYPK
                     ITKDHFAVDFICDTLRMMTMNLEDPHQVEDAMEKQLEKHHHEAHAPAHALTQLADGLP
                     ALGIVAAVLGIIKTMGAITEPPAVLGGMIGSALVGTFLGVFMAYGLVGPMATRLAAVV
                     DDDHAFYKIIQAVLVAHLHGNAAQISVEIGRGNVPTAAQPSFLELEEALSQIPNEA"
BASE COUNT          583 a         1328 c         1455 g          582 t
ORIGIN      
        1 gacgcctcgg tgaccctggg cgtgcgctat accttcggcg cgccgccgcc tcccccgccg
       61 ccgcccccgg ccccgccggc cccgccgccc gctcccccgg ccccgccggc tcccccgccg
      121 ccgccgcctg ccgcggtcta tgaagcgcgc gagttcgtgg tttacttccc gtttgatcag
      181 tacgtgctga cgcccgaagc ccaggcggtg gtccagcaag ccgccgacta tgccaaggcc
      241 ggcatggcga ccaagctgac gatcacgggt cacaccgata cgtcgggttc ggatcgctac
      301 aacgcccgtc tgtcggaacg ccgcgccaag gccgttgctg atggtctggt cggcatgggc
      361 gtcgccgcca ctgccgtggc tgtcgactgg aagggcgaaa gcgctcccgc cgtcgccact
      421 ggcgatggtg tgaaggaacc gctgaaccgt cgttcgacga tctcgatcaa cttctagatc
      481 gaacgttcat cggagtttgg ggctcgaccg aaaggtcggg cccttttctt tggccgggac
      541 cctgaaacga aagacgctcc cgatggggcg tcgggagcgt caacacggac ggcctggagg
      601 gcaagaggcc gtcgtcatgg accgaccttg gggggttgcc ggcctcatgt cactgggcgc
      661 cgtcttgcgg gcggacgccg tccgtcagag gccctcaggg cccgacgaaa gctcgttcac
      721 gcggacttac ctgtccaatt tcgaggttga ttatacgccg gctctcgcca cgggcgagtc
      781 aaaacggtcc ggcatgagca ggcgcgacaa ttcgcgcggg agcggcgagg gcgcgcccgt
      841 cacccgggcc accacgtgct cggccagcag cggcgccagg cagaagccgc gtccgcccag
      901 accgctcaga acaaacaggc cttcagccag ttggccggcc aatggcaggt gatcgggggt
      961 ggcgacccgt gtcgcggcgc gccccgacag cggaacgcgg cccagccgct cggccagttg
     1021 cggcaagccc ttggccagga tctccagatt gcgcctgtga tcttctgatc gcacgtcggt
     1081 gtcgagcacg ccccggtcat gggtggcgcc gaacagaacg ccgtcgcggg tcggaatggc
     1141 atagccgccg aaggccgccg gggtgcagcc tggctcggca acggcggtcc agctggcctg
     1201 gccgcgcacc ggtcgatagc caagggtggg ccacaggtcg cccagccccg cgccggccgc
     1261 gatgatcacg gcgtcggcgc gcaggatctc gccgccattg ccgtcgagca gacgccaggc
     1321 gtccgcatgt cgctccaggc cggccacccg cgccgccagg gtttcgcccc gccaggcgtc
     1381 cagcaccccg cgcggttcga tcaccagcgc tctggacatg accagggcgg cgcccgcggg
     1441 ctcgcccagc gggtcggcgg ccgaggtcag cagggtcatg gttccgggtt cgaagatgtc
     1501 ctggcccgcc acaaccgcaa agcgctcttc ggccggctgg ctctgggcca gttgcaggac
     1561 gccgcgggcg atcacggcgc cgggcagggc ttcatagagg tcgaccgccc gggcgaaggc
     1621 ctgggccggg agcgcggcgc gcgggccccc gccggcgtcg agagcagggg tgacgagggc
     1681 ggcgggattg cccgaggcgg gcgcgtcgtc agcgtcgatg accgagacct cgaggccctg
     1741 agcgcgggcg gcgcgggcca gggcggcggc ggcaatgccg ccgccgatga tggccagacg
     1801 ggcggggctg ggcgacgcgg acggcgcgtc ggggagccag gcctccaggc ggtccttctt
     1861 gcggccaaag ccggggcgct tggcgatctc gaagccggcc gcctgcaggc cgcgccgcac
     1921 tgcgccggcg accgtgaagg tggccgcgcg cgcgccgggc gccgagcggg cggcgacggc
     1981 ggccagaatg tcctcgctcc acattgccgg attgagggcg ggcgagaagc cgtcgaggaa
     2041 ccaggcgtcg gccgcgccgt cccagctttc cagggcctgg cgcacgtccg tgaccgccag
     2101 atccagggtg gcgcggaagc ctggcaggtc aacccggtga aagccgcgcg cttgccctgg
     2161 ccagtgatcc agcagcgcct gggcggcctc gcccagctcg ggccagaccg acagggcgcg
     2221 ggcggcgtcc tcgcggctga tcggatgggc ctcgatcgag aagatatgca gccgggcttg
     2281 cgcgggcgcc tcgcgacgcc acagatccag cagggcggcg atattgaggc cggtgccaaa
     2341 gcccagttca ccgaccacca gccggtcgcg gcccgcgaag cgctcgggca ggccgcagcc
     2401 ggtcaggaac accgcgcggg tttcggccag tccgtcggcg ctggagaaat agacgtcgcc
     2461 atacagacgc gagcgcggca ggccgtcctc gttccagacg aggggcgagg cgtcatcggg
     2521 cgaggcgtcg gtcatgggcg ctagagccgt gcgagccgcg cctgtgtcaa gcgcacacga
     2581 aaaaggggcc gcccgccagg acggcccctt tgatctcgac gccgggcgcg gcttagtgcg
     2641 cgggcttttc cggcgcggcg gcgggctggg ccggttcctt ggcggtggag gccggggctt
     2701 cggcggttgc ggtggtcgcg gcgtcgtcgc cttcggccat cggagcggcg gcggcgtcag
     2761 cggcggcctt gttggcgtca gccacggcct ggtcgtgggc ggctgcggcg tcggtcacgg
     2821 tgctggcgcc ggcggcggcg gcttcggccg ccgcggtcga ggcgtccgcg ccctcttcct
     2881 ttttgccgca ggcggcgagc gaaagggccg atacggcgac gccggcgatc agaagcgtgc
     2941 gaagaacggt ggtgttcatg ggaaacccct tgcggctgtt gttctggacg gtcgcccacg
     3001 gttcgcaggc gacgccttca gctggcaggg aataccggtt cgaacaggcg cgcaagcgtc
     3061 aggtcacgcg tccgtgatta catcgacgac agactcgcaa gcgccggacg accagacccc
     3121 gatcaggcct cgttggggat ctgcgacagg gcctcttcca gctccaggaa gctgggctgg
     3181 gcggcggtgg gcacattgcc gcgaccgatc tcgaccgaga tctgggcggc gttgccgtgc
     3241 aggtgggcga ccagaacggc ctggatgatc ttgtagaagg cgtggtcgtc atcgaccacg
     3301 gcggccaggc gcgtcgccat cgggccgacc aggccatagg ccatgaacac gcccaggaag
     3361 gtgccgacca gggccgagcc gatcatgccg cccagcacgg ccggtggctc ggtgatcgcg
     3421 cccatggtct tgatgatgcc cagcacggcg gcgacgatgc ccagggccgg caggccgtcg
     3481 gccagctgcg tcagggcgtg ggccggggcg tgggcctcgt gatggtgctt ttcgagctgc
     3541 ttctccatcg cgtcctcgac ctggtgggga tcctccaggt tcatggtcat catccgcagg
     3601 gtgtcgcaga tgaagtcgac ggcgaagtgg tccttggtaa tcttgggata gcgcgagaag
     3661 atcgagctct cgtgcggctt ttcgatgtgg ctttccaggg cgatcacgcc cttggacttc
     3721 atcgtcttgg tcagcaggaa cagcagggcc agcaggtcgc gatagtccga gttcttccac
     3781 ttggggcccg cgaagatctt gccgaagccg gccgcggtgg ccttgatcac ggtcatcgag
     3841 ttggcgatca ggaaggcggc gacgccggcc ccgagaatgg ccatcagttc gtgcggcgcg
     3901 gcgtggatga tcacgtccat cttgccgcca gccatgatga agctgccg
//
LOCUS       DAOMEO010000156         3938 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2711_length_3938_cov_2.118723, whole genome shotgun sequence.
ACCESSION   DAOMEO010000156 DAOMEO010000000
VERSION     DAOMEO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2711_length_3938_cov_2.118723"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLH31_04800"
     CDS             <1..1450
                     /locus_tag="PLH31_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HQR88579.1"
                     /translation="KGDVHDIGKNIVGVVLQCNNYEVIDLGVMVPADRILDEARKHKV
                     DMIGLSGLITPSLDEMVFVAAEMERQGFTIPLLIGGATTSRTHTAVKIEPAYSKGSTT
                     YVLDASRAVGVVSGLLSASERDRLQAETRAEYIRIREQYARGQTAKARTSLAEARQKK
                     FAIDWEGYVPPKPSFIGTRTFEPSLAELVPFIDWSPFFASWELIGRFPQILEDDKVGE
                     AATELYREAREMLDKVVAEKWFGARGVIGFWPAQAQGDDIVLYTDDTRTAELSRLHTL
                     RQQMDKGAGKTNAALADFVAPIGQGGDYVGGFAVTAGHGEDEIVKRFKDAGDDYSAIM
                     ASALADRLAEAFAEWLHYKARVELWGYAPDESADADVMIAEKYQGIRPAPGYPAQPDH
                     TEKGTLFQLLDATEQTGMILTESYAMSPGAAVSGLYFSHPKSHYFGVGKVDLDQVEDY
                     ARRKGWDLAKAEKWLAPILNYDPGARAREAAA"
     gene            1413..1991
                     /locus_tag="PLH31_04805"
     CDS             1413..1991
                     /locus_tag="PLH31_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR88580.1"
                     /translation="MIRGPGRARRRRRERDIDRFVVISGCSGGGKSTLLAELRRRGHA
                     VVEEPGRRIVAEEMAADGAALPWIDPAAFARRAIAMALADREDAPSQGWVFFDRGLID
                     AAVALEHLTGEPAVETLCGLHRYHRQVFMAPPWPEIHVVDRERRHGLDEAVAEYERLQ
                     VAYPALGYNILVLPKTSVADRANFVLDALNTL"
     gene            complement(2015..2362)
                     /locus_tag="PLH31_04810"
     CDS             complement(2015..2362)
                     /locus_tag="PLH31_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQR88581.1"
                     /translation="MNILKHNGYMARVAFDDEDEIFVGRLAGITDIISFHADTVSGLK
                     AAFHEAVADYIATCAKAGKKPEKPYSGQIMVRVDPVVHARAALAAELAGKSLAGWTEE
                     KLRDAATREIEAA"
     gene            complement(2359..2613)
                     /locus_tag="PLH31_04815"
     CDS             complement(2359..2613)
                     /locus_tag="PLH31_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQR88582.1"
                     /translation="MNSKQKKTLTAIFADPVSPTIAWVDIESLLISAGARVIEGSGSA
                     VRFECNGVIARFHRPHPDKEAKRYQVKDARAFLTAIGVKP"
     gene            2919..>3938
                     /locus_tag="PLH31_04820"
     CDS             2919..>3938
                     /locus_tag="PLH31_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HQR88583.1"
                     /translation="MNRNNIELKIFGLIVGMAAAMVLVAAGLLFTATRQADLRERQYE
                     QTLVSNGLRQRGKEVQTSLNPYVIWDEAIHKLDNKFDLAWANQNIGTSIGGALGYSVV
                     FVLDADNRPIYGHMNGTDVSPDVFGTYAAQTQRLIQMVRAIEADPVKVAEMRAVHVSR
                     VELIGREPVMITVSLVRPDLKAKTRGVRAPLVVSGMPVASSVVRAFSERFLLKNARIE
                     IVPAAEIKGENAQALMGRAPDGRGIVLRWDPRRPGAELLQQATPILALAALAAALGAG
                     LLFRVTRRAAKKLLLSEAEAKHLALHDPLTGLANRTLFTDRLVHAHALLRRKPGYLGV
                     LCVDLD"
BASE COUNT          680 a         1304 c         1279 g          675 t
ORIGIN      
        1 caagggcgac gtccacgaca tcggcaagaa catcgtcggc gtggtcctgc agtgtaacaa
       61 ctacgaggtc atcgacctgg gcgtcatggt tccggccgac cgcatcctcg acgaggccag
      121 gaagcacaag gtcgacatga tcggcctgtc gggcctgatc accccctcgc tggacgagat
      181 ggttttcgtc gccgccgaga tggaacgcca gggcttcacc atcccgctgc tgatcggcgg
      241 cgccaccacc agccgcaccc acacggcggt caagatcgag ccggcctatt ccaagggctc
      301 taccacctat gtgctcgacg ccagccgcgc cgtgggggtg gtgtcgggcc tgttgtcggc
      361 cagtgagcgc gatcggctgc aggccgagac ccgggccgag tacatccgca tccgcgagca
      421 atatgcccgg ggccagacgg ccaaggcgcg caccagcctg gccgaagccc gccagaagaa
      481 gttcgccatc gactgggaag gctacgtacc gcccaagccc agcttcatcg gcacgcgcac
      541 cttcgagccg agcctggccg aactggtccc cttcatcgac tggtcgccgt tcttcgccag
      601 ctgggagctg atcggccgct tcccgcagat cctggaagac gacaaggtgg gcgaggccgc
      661 caccgagctc tatcgcgaag cccgcgaaat gctcgacaag gtggtcgccg agaaatggtt
      721 cggcgccagg ggcgtgatcg gcttctggcc ggcccaggcc cagggcgacg acatcgttct
      781 ctatacggac gacacccgca ccgccgagct gtcacgcctg cacaccctgc gccagcagat
      841 ggacaagggc gccggtaaga ccaacgcggc gctggccgac ttcgtcgcgc cgatcgggca
      901 ggggggcgac tatgtcggcg gcttcgccgt caccgccggc catggcgaag acgagatcgt
      961 caagcgcttc aaggacgccg gcgacgacta cagcgccatc atggccagcg ccctggccga
     1021 ccggctggcc gaagccttcg ccgagtggct gcactacaag gcccgcgtcg agctatgggg
     1081 ctacgccccc gacgagtccg ccgacgctga tgtgatgatc gccgagaagt atcagggcat
     1141 ccgccccgcg cccggctatc cggcccagcc cgaccacacc gaaaagggca cgctgttcca
     1201 gctgctggac gccaccgagc agaccggcat gatcctgacc gaaagctacg ccatgagccc
     1261 cggcgcggcg gtctcgggtc tctatttcag ccaccccaaa agccactatt tcggcgtcgg
     1321 caaggtggat ctggaccagg tcgaggacta cgcccgccgc aagggctggg acctggccaa
     1381 ggccgagaag tggctggcgc cgatcctgaa ctatgatccg ggggccaggg cgcgcgaggc
     1441 ggcggcgtag ggagcgcgac atcgaccgct tcgtcgtcat ctccggctgt tcgggcggcg
     1501 gcaagtcgac cctcctggcc gagttgcgtc ggcgcggtca cgcggtggtc gaggagccgg
     1561 ggcgcaggat tgtcgcagaa gaaatggccg ccgacggcgc ggccttgccg tggatcgatc
     1621 cggccgcctt cgcccgtcgg gccatcgcga tggccttggc ggatcgagag gatgccccat
     1681 cgcagggctg ggtgttcttc gatcggggcc tgatcgacgc cgccgtcgcc cttgagcacc
     1741 tgacgggaga gccggccgtt gagaccttgt gcggtctcca tcgctatcat cggcaggttt
     1801 tcatggcccc gccctggccg gagatccatg ttgtcgaccg cgagcggcgg cacggcctgg
     1861 acgaggctgt cgccgagtat gaacggcttc aggtggcgta tcccgcgctt ggttataaca
     1921 ttctggtcct gcccaaaacg agcgttgccg accgagccaa cttcgttctc gatgccctga
     1981 acacattgta gaaaagcctg cggcggtcac ggtctcaagc cgcctcgatc tcccgcgtcg
     2041 ccgcgtcccg cagcttctcc tccgtccatc ccgccaggct cttgcccgcc agttccgccg
     2101 ccagcgccgc gcgggcgtga acgacgggat cgacgcggac catgatctgg ccggaatagg
     2161 gcttttcggg cttcttgccg gccttggcgc aggtggcgat atagtcggcg acggcttcgt
     2221 ggaaggccgc cttaagtcca ctaacggtgt cggcgtggaa gctgatgatg tcggtgatgc
     2281 cggccaggcg accgacgaag atctcatcct cgtcgtcgaa cgcgacgcgg gccatgtagc
     2341 cgttgtgctt caggatgttc atggcttcac tccaatcgcc gtaaggaacg cgcgggcgtc
     2401 cttgacctga taccgcttgg cctctttgtc cggatgcggc cggtggaagc gggcgatcac
     2461 gccgttgcac tcgaaccgaa ccgcggatcc cgagccttcg atcacccggg cgccggcgct
     2521 gatcagcagg ctttcaatgt cgacccacgc gatggtcggc gagacgggat cggcaaagat
     2581 agccgtcagg gttttctttt gcttgctgtt catgcaagca gtgtgtttgt gatagcaaat
     2641 tgtgcaagcg tcaagcgggc ttcctgtcct ctcagcaagt gactgctcaa taggatggcc
     2701 caggcccaag tcccgcaagg gttgtttaca ctcatagcgt gtaaataagg gtattgtgcg
     2761 cccgctgccc aggcaatttt ctggcccgtc cagccccaaa ttgggtattc gaccctcatc
     2821 gccgaacaat gtgcgacaaa atctccggaa agccgtcgcg gtaaggaatt cagcaactgc
     2881 tctcgtgtgg tgatgcgcgc gtctgtcggg acccgcccat gaaccgcaac aacatcgagc
     2941 tgaagatctt cggcctcatc gtgggcatgg cggccgccat ggtcctggtg gccgctggtc
     3001 ttttgttcac cgcgacgcgt caggccgacc tgcgcgagcg ccagtacgaa cagaccctgg
     3061 tcagcaatgg tcttcgccag cggggcaagg aggttcagac ctcgctcaat ccctatgtga
     3121 tctgggatga ggcgatccac aagctggaca acaagttcga cctcgcctgg gccaaccaga
     3181 atatcggaac ctcgatcggc ggggccctgg gctacagcgt cgtcttcgta ctcgacgccg
     3241 acaacaggcc gatctacggc cacatgaacg gcacggacgt gtcgccggac gttttcggga
     3301 cctatgccgc ccagacccag cgcctgatcc agatggttcg cgcgatcgaa gccgatccgg
     3361 tgaaggtcgc tgaaatgcgc gccgtgcatg tcagccgggt cgaactgatc ggccgggagc
     3421 cggtgatgat caccgtgtcg ctggtgcgtc ccgacctgaa ggccaagacc cgcggagtgc
     3481 gggcgccgct ggtggtgtcc ggcatgccgg tggccagcag cgtggtccgg gcgttttccg
     3541 agcggttcct gctcaagaac gcccgtatcg agatcgtccc ggccgccgag atcaagggcg
     3601 agaacgccca ggccctgatg ggacgggcgc ccgatggtcg cggcatcgtg ctgcgttggg
     3661 atccgcgcag gcccggcgcc gaattgctgc agcaggcgac gccgatcctg gccctggccg
     3721 ccctggccgc cgccctcggc gcggggctgc tgttccgggt cacccgccgc gcggccaaga
     3781 agctgctgct cagcgaggcc gaggccaagc acctggccct gcatgatccg ctgacgggcc
     3841 tggccaaccg caccctgttt accgaccgcc tggtccacgc ccacgccctg ttgcggcgca
     3901 agcccggcta tctgggcgtg ctgtgcgtcg atctggac
//
LOCUS       DAOMEO010000157         3936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2713_length_3936_cov_2.358413, whole genome shotgun sequence.
ACCESSION   DAOMEO010000157 DAOMEO010000000
VERSION     DAOMEO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_3936_cov_2.358413"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PLH31_04825"
     CDS             <1..1337
                     /locus_tag="PLH31_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HQR88584.1"
                     /translation="GIYGGVWVGKDSPIPNTRGIRKDVVEALRAIKAPNVRWPGGCFA
                     DEYHWRHGIGPAENRRKTINSNWGGSVEPNTFGTDEFMDFVEQIGSEAYISVNVGSGT
                     VAEAAEWIEYMTADPATTAGKERVANGRTEPYKVKYLGIGNESWSCGGAMRAEYYTDE
                     MKRYARFVRNYNPKQTGAEAMQRIAVGPNQGDTAYTEAVMAAQKGHDWAWNIEGLSLH
                     SYTTGGWPPSHSANMDKYDPEKKVPLAVDEWGVWLAPLPNTNPGFLAQQNTLRDGIIA
                     ALNINIFARHADRVRVANIAQMVNVLQAMILTDNDKMVLTPTYHVFKMYVPFQDATLI
                     PVSFDAGTYVQGDIKLPRVDAVAARDTQGKLWLAVTNLDPTRPASVLADVAGARATSA
                     RGQVLTAPRVDALNTFAAPNAVMPKPYAAKARAKGLNLDIPAKSIVVVQLEP"
     gene            1453..2190
                     /locus_tag="PLH31_04830"
     CDS             1453..2190
                     /locus_tag="PLH31_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQR88585.1"
                     /translation="MPLDEPDDKLITRSARGSGRRLRGAIAHYLGTAIVSGQIAPGEK
                     LTGEIANAEALDVSRSAYREAVQVLTAKGLVESRPNAGTRVLPRSRWNILDPAVLAWA
                     FSAEPDVAFVRDLFELRAVVEPAAARFAAERRDKDDIKAMKDALAGMRRHTLATEAGR
                     AADRAFHDALLHATHNNAMIALSASISAAVSWTTEFKQRSRALPRDPVPDHARVCDAI
                     VAGDPEAASAAMRTLVDLALEDTRSAM"
     gene            complement(2203..3684)
                     /locus_tag="PLH31_04835"
     CDS             complement(2203..3684)
                     /locus_tag="PLH31_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQR88586.1"
                     /translation="MWRSDLGDGTYRNPPLNADYPDPDIIRVGEDFYFASTTFVNTPG
                     LTILHSRDLVNWDIAGHVMPRLEGSPKYDLGDGGDYRRGVFAPSLRHHNGRFYIAVTP
                     VGQNTRIYSAASIGGPWTTRELDREAFDPGLFIDRDGRAFIATAAGSDGTVRLLTLNA
                     DLSAVTGEQKIHYVLGAEGSKLIRRGDWYYLFNAIPRRLALTVSRSKSLTGPWETREQ
                     IDDTTGGHQGALVDLPDGGWYGFVMLDAGAIGRVTNISPVFWRDDWPVWGTPEAPGRV
                     PDVAAKPILGKPFVEPPSSDDFGGSTLGRQWQWNHNPDDSRWSLTERPGFLRLKATTS
                     QDFWTARNTLIQKGQAPGSRGVVKLDVRGLAPGDACGFGTFGKFSNQLVVTRTPEGRG
                     ALSARVVESAEAGPRTEARGETRAIPLTDLWLSVEMDFKTDKTALAYSLDGQSWTAMP
                     GDYPLAFAWRTGTFQGEQFGLFCHNPGGGAGRIDVDSFTLTRP"
BASE COUNT          701 a         1307 c         1306 g          622 t
ORIGIN      
        1 caggcatcta tggcggcgtc tgggtgggta aggactcgcc gatccccaat acgcgcggca
       61 ttcgcaagga cgtggtcgag gccctgcggg cgatcaaggc gcccaatgtc cgctggccgg
      121 gcgggtgctt cgccgatgag tatcactggc gccacgggat cggtccggcc gagaaccgtc
      181 gcaagaccat caattccaac tggggcggct ctgtcgaacc caacaccttc ggcaccgacg
      241 agttcatgga cttcgtcgag cagatcggca gcgaggccta tatctcggtc aatgtcggct
      301 cgggcacggt cgcggaagcc gccgagtgga tcgagtacat gacggccgat ccggccacca
      361 cggcgggcaa ggagcgcgtc gccaacggcc gcaccgagcc ttacaaggtc aagtatctgg
      421 gcatcggcaa tgaaagctgg agctgcggcg gcgcgatgcg cgccgaatac tatacagatg
      481 agatgaagcg ctacgcccgc ttcgtgcgca actacaaccc caagcagacc ggcgccgagg
      541 ccatgcagcg catcgccgtt ggccccaacc agggcgacac ggcctatacc gaagcggtga
      601 tggcggcgca gaaggggcat gactgggcct ggaacatcga aggcctgtcc ctgcactcct
      661 acacgaccgg cggttggcct ccatcccatt cggcgaacat ggacaagtat gatcccgaaa
      721 agaaagtgcc gctcgccgtc gatgagtggg gcgtctggct ggcgccgctg cccaacacca
      781 atcccggctt cctggcccag cagaatacgc tgcgagacgg gatcatcgcc gcgctgaaca
      841 tcaacatctt cgcgcgtcac gccgaccggg tgcgcgtggc caacatcgcc cagatggtca
      901 acgtcctgca ggcgatgatc ctgaccgaca acgacaagat ggtcctgacc ccgacctatc
      961 atgtcttcaa gatgtacgtg ccgttccagg atgcgaccct gatcccggtc agtttcgacg
     1021 ccgggaccta tgttcagggc gacatcaagc tgccgcgcgt cgacgccgtg gccgcgcgcg
     1081 acactcaggg caagctctgg ctggcggtga cgaacctcga cccgacacga cccgcttcgg
     1141 ttctggcgga tgtcgccggc gccagggcca ccagcgcgcg cggtcaggtg ctgaccgcgc
     1201 cccgcgtcga tgcgctcaac acgttcgccg cgcccaacgc ggtcatgccc aagccctatg
     1261 ccgccaaggc gcgcgccaag gggctgaacc tggacattcc ggcaaagtcg atcgtcgtcg
     1321 tgcagctgga gccatagcgc cgacggcggg aggccgcgcc agaaccgcga atccggtcag
     1381 cccagacaaa cttgccaagg ctgcggtgct tgcgcttata ggactgcgta tgcagcccag
     1441 agccctatgc ccatgccgtt ggacgagccg gacgacaagc tgatcacccg atcggcaaga
     1501 ggctcggggc ggcgacttcg cggcgcgatc gcgcactatc tgggcacggc gatcgtctcg
     1561 ggccagatcg cgcccggcga aaagctgacc ggcgagatcg ctaacgccga ggcgctggac
     1621 gtgtcacgca gcgcctatcg cgaggcggtt caggtgctga ccgccaaggg cctggtggaa
     1681 agtcggccca acgccggcac gcgcgtcctg ccgcgcagcc gctggaacat cctggatcct
     1741 gcggttctgg cctgggcgtt ctcggctgag cccgacgtgg ccttcgtgcg cgacctgttc
     1801 gaacttcggg ccgtggtcga acccgccgcc gcgcgcttcg ccgccgagcg tcgcgacaag
     1861 gacgacatca aggcgatgaa ggacgccctg gccggaatgc gccgccacac attggcgacg
     1921 gaagccggtc gcgcggccga ccgggcgttc cacgacgccc tgctacatgc cacgcacaac
     1981 aatgcgatga tcgccctgag cgccagcatc agcgcggcgg tcagctggac gaccgagttc
     2041 aagcagcgct cgcgcgccct gccccgcgat cctgtgcccg atcacgcccg ggtctgtgac
     2101 gcgatcgtcg ccggcgaccc cgaagcggcc agcgccgcca tgcggacact ggtcgacctg
     2161 gcgctggaag atacgcgctc ggccatgtga ccgccgtgct ggctagggtc gggtcagcgt
     2221 gaagctgtcc acatcgatcc gtcccgcgcc gccgccggga ttgtggcaga agagcccaaa
     2281 ctgctcaccc tggaaggtgc cggtgcgcca ggcgaaagcc agcgggtagt cgcccggcat
     2341 ggccgtccac gactggccgt cgagactgta ggccagggcc gtcttgtcgg tcttgaagtc
     2401 catttcgacc gacagccaca gatcagtcag cgggatggcg cgcgtctcgc cccgcgcctc
     2461 tgttcggggc ccggcctcag cgctttcgac cacccgcgcg ctcagggctc cccgcccctc
     2521 cggcgtgcgc gtcaccacca gctggttgga gaacttgccg aacgttccaa agccgcaggc
     2581 gtcgccgggc gccaggccac ggacgtccag cttgaccacg ccgcgactgc cgggcgcctg
     2641 acctttctgg atcagggtgt tgcgggcggt ccagaaatcc tggctcgtcg tcgccttcag
     2701 ccgcaggaag ccgggacgct cggtcagcga ccagcggctg tcgtcgggat tgtggttcca
     2761 ctgccattgc cggcccagcg tggagccgcc gaaatcgtcc gagctgggcg gctcgacgaa
     2821 gggcttgccc aggatcggct tggcggcgac atcggggacg cgaccggggg cctccggcgt
     2881 gccccacacc ggccagtcgt cgcgccagaa caccgggctg atattggtga cccggccaat
     2941 ggcgccggcg tccagcatga caaagccata ccagccgccg tccggcagat cgaccagggc
     3001 cccctggtgc cccccggtgg tgtcgtcgat ctgttcgcgg gtctcccagg ggcctgtcag
     3061 gcttttggac cgcgacacgg tcagggccag gcgccgggga atggcgttga acagataata
     3121 ccagtcgccg cggcggatca gcttggagcc ctccgcgccc agcacatagt ggatcttctg
     3181 ctcgccggtg acagccgaga ggtcggcatt gagcgtcaac agacggaccg tgccatccga
     3241 accggccgct gtggcgatga aggcccggcc gtccctgtcg atgaacaggc ccgggtcgaa
     3301 cgcctcccgg tccagctcgc gcgtggtcca gggtccgcca atgctggcgg cggaatagat
     3361 acgcgtgttc tgccccaccg gcgtgacggc gatatagaag cggccgttgt ggtggcgaag
     3421 gcttggggcg aacacgccgc gccgatagtc tccgccatcg ccgagatcat atttggggct
     3481 gccttcaagg cgcggcatca catgaccggc gatgtcccag ttcaccagat ccctggaatg
     3541 caggatggtc aggccgggcg tattcacaaa ggtcgttgag gcgaagtaga aatcctcacc
     3601 aacgcggatg atgtcgggat cgggatagtc ggcgttgagc ggcgggttgc gataggttcc
     3661 atcgcccaga tcggaccgcc acacttggcc ctgcgcggcg gtcgcggcgg cgagcgcgag
     3721 gccagcggcg gcggccagga ttctgctgcg gatcgacctg ataatggaac cctcccggcg
     3781 gcgcccggaa ggcgctctaa ttgtctgagt atatagtctg aaaatcggcg tcaacgacgc
     3841 cctgaacgcg cgtgttcggt ggggagaatt tttcagacat caatgagagc ggtatcattc
     3901 agagcttgga gtgattgcgc gcggcaattt tgtctg
//
LOCUS       DAOMEO010000158         3929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2717_length_3929_cov_2.599380, whole genome shotgun sequence.
ACCESSION   DAOMEO010000158 DAOMEO010000000
VERSION     DAOMEO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_3929_cov_2.599380"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLH31_04840"
     CDS             complement(<1..371)
                     /locus_tag="PLH31_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQR88587.1"
                     /translation="MRSMLLTLVMSVLVAAIGVWGGAQFVMHRMKQPTPLHELVHEKL
                     GLSSEQHARIAGIEREHEAKRQALEAEMRAANAELARAFQQKHAYTPEVQAAIDRFHH
                     AMGELQTETMVHTLAMRAVLTP"
     gene            complement(377..670)
                     /locus_tag="PLH31_04845"
     CDS             complement(377..670)
                     /locus_tag="PLH31_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88588.1"
                     /translation="MSERLDELVARLAASPTDRSLDGLDGEVGRSIAHRRREVRTLAA
                     LAPVRVASVGLALAMGVTAGGAVAASALVGPHPYGTFSVAANLAPSTLLEGRE"
     gene            complement(762..1103)
                     /locus_tag="PLH31_04850"
     CDS             complement(762..1103)
                     /locus_tag="PLH31_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQR88589.1"
                     /translation="MIRKTTFLAVAIALTLGVATASAQSGDHNMMAMNDYMAVMKKMD
                     QAMMTAKGATADQTFARKMIAHHRGAIEMSQIQLRHGTDAEAKRLAQKTIDENTKGIA
                     EMESWLKSHGG"
     gene            1388..1810
                     /gene="cueR"
                     /locus_tag="PLH31_04855"
     CDS             1388..1810
                     /gene="cueR"
                     /locus_tag="PLH31_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HQR88590.1"
                     /translation="MNIGQASNASGVTAKMIRYYEQIGLVRPADRTDSNYRSFGERDI
                     NDLRFIKRARSLGFSVKEITSLLSLWRDRARPSREVKGIADGHVADLRSRIAEMQAMA
                     DTLSALSDCCAGDDRPDCPILSDLSGDKPAKGPAQSPS"
     gene            1840..>2159
                     /locus_tag="PLH31_04860"
     CDS             1840..>2159
                     /locus_tag="PLH31_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88591.1"
                     /translation="MLPTASLAAPTDYRFEAVTDTVVRGAGVPLRVRALYSANGQPAP
                     SIEVRDARVDRSPDGQVGGSHPAFFTPSLDYGVYGFRADLPVDGRWALTFTARIPGEP
                     QPVTA"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2242..2571)
                     /locus_tag="PLH31_04865"
     CDS             complement(2242..2571)
                     /locus_tag="PLH31_04865"
                     /inference="COORDINATES: protein motif:HMM:NF023036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HQR88592.1"
                     /translation="MKFVLAAAASLALSSAAYAQADHDMSKMPGMAMPAPAGAQGAGV
                     VKKIDPRAGSVTLQHGPIPVLSWPAMTMAFKADSALLKTVKVGQQVNFTVKTGGAAPE
                     VVAIQPK"
     gene            complement(2649..>2841)
                     /locus_tag="PLH31_04870"
     CDS             complement(2649..>2841)
                     /locus_tag="PLH31_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88593.1"
                     /translation="TMRNIRQNLVFAFAYNVAGIPIAAGLLYPAFGWLLSPAIAAAAM
                     ALSSVSVIANALRLRTVRL"
     assembly_gap    2842..2851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2852..3929)
                     /locus_tag="PLH31_04875"
                     /pseudo
     CDS             complement(2852..3929)
                     /locus_tag="PLH31_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521615.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT          646 a         1331 c         1279 g          653 t
ORIGIN      
        1 ggcgtcagaa cagcgcgcat ggccaaggtg tgaaccatgg tctccgtctg gagctcaccc
       61 atggcgtggt ggaaacggtc gatcgccgcc tgcacttcgg gcgtataggc gtgcttctgt
      121 tgaaaggcgc gggcgagctc ggcgttggcg gcccgcatct cagcctcgag cgcctggcgc
      181 ttggcctcat gctcccgctc gatcccggcg atgcgcgcgt gctgttcgct gctgaggcca
      241 agcttttcgt gcaccagctc atggagcggc gtgggctgct tcatgcggtg catgacaaac
      301 tgcgcaccgc cccacacacc gatggcggcg accagcaccg acatcacgag cgtcagcagc
      361 atgctgcgca tcaggctcac tcgcggccct ccagcagcgt cgaaggcgcg agattggcgg
      421 ccacggagaa ggtgccgtag gggtggggcc cgacaagcgc cgacgcggcg acggcgccgc
      481 ccgcggtgac gcccatggcg agcgcgaggc cgaccgaggc gacacgaacc ggcgccaagg
      541 ccgccagggt tcgcacctcg cgacgccggt gcgcgatgct gcgcccgacc tcgccatcaa
      601 ggccgtccag ggatcgatcg gtgggacttg ccgccaatcg cgcgacaagt tcgtccaggc
      661 gttcactcat gtacaagtcc tcaggcgtcc ctgaagacta tacgtgccag accacgacca
      721 acccctaaag gcccatcgac ttggcgccgg ccgaagggcc ctcagccgcc gtgcgacttc
      781 agccagctct ccatctctgc gatacccttg gtgttctcgt cgatggtctt ctgggccaac
      841 cgcttggctt cggcgtccgt gccgtggcgg agctggatct gggacatctc gatagcgccg
      901 cgatggtgag cgatcatctt gcgcgcgaag gtctggtcgg ccgtggcccc cttggccgtc
      961 atcatcgcct ggtccatctt cttcatgacg gccatgtagt cgttcatggc catcatgttg
     1021 tggtcgcccg attgggctga cgcggtcgcc accccaaggg tcaaggcaat cgccacagca
     1081 aggaacgtgg tcttacggat catcatcgag actcctgggt gtcgaacgcc catcgcgcca
     1141 gaccttgaga actccaaccg tccaaacctc cctttgttgc atccgcgtcg accgctaaaa
     1201 ccttccaatc attagaactt ttccgatttc taggccctcg cgcagagctt ccgcgcttag
     1261 tttcagggac gcgctcgacc tgatgcgtat ccctgaagga ggcggagcct tgaccttcca
     1321 actgtgggaa ggattaaggc tgggcgtggt cgccgcgcgg ccggggagag ttgcgccagg
     1381 aggcgggatg aatatcggtc aagcctcgaa cgcctccggc gtcaccgcca agatgattcg
     1441 ctactatgag cagatcggcc tcgttcgacc ggcagaccgc accgacagca attatcgcag
     1501 cttcggcgaa cgcgacatca acgacctgcg gttcatcaag cgggcgcgct cgctgggctt
     1561 ctccgtcaaa gaaatcacca gcctcctctc gctgtggcgg gaccgggccc ggcccagccg
     1621 cgaggtgaag ggcatcgccg acggccacgt cgccgatctg cgaagccgca tcgccgagat
     1681 gcaggccatg gccgatacgc tgagcgcgct gtccgactgc tgcgccggag acgacaggcc
     1741 ggactgcccg atcctcagcg acctctccgg cgacaagccc gccaagggcc cagcgcagag
     1801 ccccagctga tgcgatcctg gtggctggtg ggactggcta tgctgccgac cgcaagcctc
     1861 gccgcgccaa cggattatcg attcgaagcc gtcaccgaca cagttgtgcg cggcgccggg
     1921 gtgccgcttc gcgtgcgcgc gctctattcg gccaacgggc agccggcgcc aagtattgaa
     1981 gtccgcgatg cgcgggtcga cagatcgccg gacgggcagg tcggtggaag ccatccggcg
     2041 ttcttcacgc ccagtctcga ctacggcgtt tacggcttcc gcgctgatct gcccgtcgat
     2101 ggccgttggg cgctgacctt cacggcccgc atcccaggcg agccccagcc cgtgaccgcn
     2161 nnnnnnnnng cgtcgtgttt acggtcgtcg ggcggctgcc ggcgccgaca gcgccaacag
     2221 ccccgacacg tcgctgaccg gctatttcgg ctggatcgcc acaacctcag gggcggcccc
     2281 gcctgtcttg acggtgaagt tcacctgttg gccgaccttg acggtcttga gcagggcgga
     2341 atcggccttg aacgccatgg tcatggcggg ccagctgagg acggggatgg ggccatgctg
     2401 cagcgtcaca gagcccgccc tggggtcgat tttcttgacc acgccagcgc cctgagcgcc
     2461 ggcgggcgcc ggcatagcca ttccgggcat cttgctcatg tcatggtccg cctgggcgta
     2521 ggcggcgctg ctgagcgcca ggctcgcggc ggcggcaagg acaaatttca ttgtagatct
     2581 ccgcgattga aagcagccgc ttggcggccg gttgttgtgt gggatacgcg ccagctcacc
     2641 gcccaccctc agagccgcac tgtgcgcaag cgcagggcgt tggcgatgac gctgacgctg
     2701 gacagcgcca tggcggcggc ggcgatggcg ggggacaaga gccatccgaa ggcggggtag
     2761 agaagccccg cggcgatcgg aatgcccgcg acgttgtagg cgaaggcgaa caccaggttc
     2821 tggcggatgt tgcgcatcgt cnnnnnnnnn ngacagccgg cgggcgcgga ccaaaccttc
     2881 gaggtcacca tgcaacaagg tcaccccggc gctttcaatg gcgacgtccg cgccggcgcc
     2941 catggcgata ccaacgtccg cggcggcgag cgccggggcg tcgttgaccc catcgcccgc
     3001 catgccgacc acgcgtcctt cggcccgcag acgctcgacg acggcggcct tgtcctgtgg
     3061 cagcacttca gcctccacct catcgaggcc gagctcggcg gcgacagcct gggccgtgat
     3121 gcggttgtcg ccggtcatca tcaccagccg gatgccttca gccttaaggc tacggatcgc
     3181 cgcggcggtc gtcgccttga tcgggtcggc gatggccaga acggctgcga acttcccatc
     3241 gacggccatc atcaccgtcg tggcgccgct agcgcgaagg cggtcgatgt ctgcgtccag
     3301 ggccgtggtt tcgacgccgt gctccgtcat cagccgcgtt ccgccaagca cgatcgcctt
     3361 gccatcgaca cggccgagca cgcctttgcc cactggcgag tcaaactccg ccgcctcgga
     3421 aagggtgagc ccgcgcgcct tggccgcccg taggatcgca tcggccaggg ggtgttcgct
     3481 gccgcgttcc aggctcgccg ccagccgcag aacctcgtcc tcggcgaagc ccggcgtcgg
     3541 ccggatctcg gtcactgccg gccgcccctc ggtcagggtg cccgtcttgt cgatgatcag
     3601 cgtgtcgatc ttttcgaaac gctccagggc ttcagcgttt ttaatgagga cgccggcctg
     3661 tgcgccccgg ccgacgccaa ccatgatcga gatcggggtg gccaaaccaa gcgcgcaggg
     3721 acaggccacg atcagcaccg agaccgccgc gatcaaggcg tagccgaagc gcggctctgg
     3781 cccgaccagg ccccagacga tcgcggcgac ggccgcgacg gcgatgaccg tcggcacgaa
     3841 ccacccctac accttgtcag cgaggcgctg gataggggcg cggctgcgct gcgcctgggc
     3901 gaccatctgc acgatctgtg agagcaggg
//
LOCUS       DAOMEO010000159         3912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2731_length_3912_cov_2.858180, whole genome shotgun sequence.
ACCESSION   DAOMEO010000159 DAOMEO010000000
VERSION     DAOMEO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_3912_cov_2.858180"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLH31_04880"
     CDS             <1..514
                     /locus_tag="PLH31_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQR88594.1"
                     /translation="NADYRPLSYYPLSLWPWQEVIKAVFLERVEVVSTYDHVVHSPSF
                     EMKLPSVVSLKQYVPQDRPPAFTRFNLFLRDTFSCQYCSSPDDLTFDHVIPRSRGGRT
                     TWENIVTACAPCNLRKGGRTPREAGMHLFHTARRPSMHELQDRGRRFPPGHLHASWLD
                     YLYWDIELEA"
     gene            complement(528..836)
                     /locus_tag="PLH31_04885"
     CDS             complement(528..836)
                     /locus_tag="PLH31_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88595.1"
                     /translation="MTDTKQTQDASAPGVLGDWSQAQDLGQTGHPEDRVSEADVKEAF
                     QKEDTPASRVVEPLPEGEKHDQLTDHVDLAEDRQEALLDEGLEESFPASDPVSVKHIT
                     "
     gene            945..1166
                     /locus_tag="PLH31_04890"
     CDS             945..1166
                     /locus_tag="PLH31_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88596.1"
                     /translation="MTNRPTALERAFQLARSGEYSTISDIRRQLKIEGFSDAPSQISG
                     PSMLKQLRKLCETARPEEAAVVEPVLDAQ"
     assembly_gap    1313..1322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1350..1871)
                     /locus_tag="PLH31_04895"
     CDS             complement(1350..1871)
                     /locus_tag="PLH31_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQR88597.1"
                     /translation="MLTPNHIVITARAVLLLGALTAAILMLGPFQGAEAAFGLNDKSA
                     HAIAFGGLTAVSFAAFPRMRRWDLARAALLLGGGAELVQMLGDRSASLGDWAADAVGV
                     FTVYGSSLIESARKMAREHGAVPFSTIAEMDRRRPRAAKPVLTPVASHGEERLARFAD
                     RAAKRYPPRASAP"
     gene            complement(1985..3784)
                     /locus_tag="PLH31_04900"
     CDS             complement(1985..3784)
                     /locus_tag="PLH31_04900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQR88598.1"
                     /translation="MPRSTLAAASAVCLLALGAGQALAQTKAPAKKTTALTLSTGGVA
                     PAEQRDMSLEHVDLRIKVIPERKAIEGDATLTILARAPLTRLILDLDRDLPISLLTID
                     GAPLAASAWSNPEGRLSITLPMPLAKGEQVKVRIQYAGVPHEAKRPPWDGGFVWKKTK
                     TGEPWIATAVQGDGCDLFWPCIDQPTGEPKLVDLHIVVPAPLVAPANGVFVGMEEKDG
                     WRTYSWRAKNPNTYAIALDIGPYVELSDTYNSRYGDSFPIRFWHLKGRETEAKALFAE
                     FGPMLDFYEAMIGPYPFRDEKMGVVETPHLGMEHQTINAYGNEYAKDVYGYDWLLHHE
                     LAHEWFGNQLTNVDWDDMWLHEGLGTYMQPLYSQWLHGDMEYMARLNTQRATLQNKFP
                     VVAGKSMAEDQVYDPKVGPGNDIYYKGSNALHTLRSLIGDEAFFKAIRLVVYGRTDPK
                     PGNFAPRYANTRDFIRAVNDVTGQDYGWFFDVYLYEASPPELMESREGDDLMLRWKTP
                     KDKPFPMPVEVKVGGRMVALPMADGSGRVRIGTAAPVIVDPASKILRRQPHIEAYQVW
                     KKAKDDAAKAEADAKKKAEEAARAKAKAKARKR"
BASE COUNT          646 a         1225 c         1277 g          754 t
ORIGIN      
        1 gaacgccgat tatcggcccc tcagctacta tccgctgtcc ctctggccct ggcaggaggt
       61 gatcaaggcg gtgttccttg aacgggtcga ggtcgtttcg acctacgacc acgtcgtcca
      121 ttcgccgtct ttcgaaatga agctgcccag cgttgtttcg ctgaagcagt atgtcccgca
      181 ggaccggccg ccggccttca cgcgcttcaa cctgtttctg cgtgacacct tctcttgcca
      241 gtattgctcg tcgcccgacg acctgacctt cgaccatgtc atcccgcgct cacggggcgg
      301 acgcacgacc tgggagaaca tcgtcacggc ctgtgcgccc tgcaatctgc ggaagggcgg
      361 gcgaacgcct cgcgaggcgg gtatgcacct gttccacacg gcgcgccggc ccagcatgca
      421 tgaacttcag gatcgcggcc gccgcttccc gccaggccac ctgcacgcca gctggctcga
      481 ctatctctac tgggatatcg agctggaggc gtgaggcggc tctagtgtca ggtgatgtgc
      541 ttgacgctga cagggtcgct ggccgggaag ctttcttcca ggccttcgtc tagcagggct
      601 tcctggcggt cttcagccag atcgacatgg tcagtcagct ggtcgtgctt ttcaccttcg
      661 ggcaaaggtt caaccacgcg gctggcaggg gtgtcttcct tctggaaggc ctccttgacg
      721 tcggcctcgc tgacgcggtc ttcgggatgg ccggtttggc ccaggtcctg agcctgtgac
      781 cagtcgccga gtacgccggg cgctgacgcg tcttgtgtct gcttggtgtc ggtcatgatc
      841 aggtcctctg atgaggaggg ctaacgtttc gcagctccgc ttgttccgaa gggcccgaca
      901 gaacaacatc tcgccgttga ggtccgtctc ctgtactgtg aggcatgacc aatagaccaa
      961 cggctctcga acgcgccttc cagctggcgc ggtccggcga atattcaacg atctccgaca
     1021 tccggcggca gttgaagatc gaaggctttt ccgatgcgcc aagccagatc agcggcccgt
     1081 ccatgctgaa gcagcttcgt aagctttgcg agaccgccag gccggaagag gccgcagtgg
     1141 tcgagccggt gctggacgcg caatagatgg cgctgtccga cggtcagctg acggctctga
     1201 agaacctggc tcgcaagcag gcgggcgatg atgtcgactg gatcaatatc agcgacgccc
     1261 gcgctctgac cgacctgggc tttgcccagc gcgatcgggt gggctggaag atnnnnnnnn
     1321 nnggggcttg aggctctggc agcggcgtct tagggggctg aagcgcgcgg cggatagcgc
     1381 ttggcggcgc ggtccgcaaa gcgcgccagg cgttcctccc cgtgcgaggc taccggcgtc
     1441 agcacgggtt tggccgcgcg tggccgccga cgatccatct cggcaatggt cgagaaaggc
     1501 acggcgccgt gttcacgcgc cattttccgc gcgctttcga tcaggcttga gccgtaaacg
     1561 gtgaagacac ccacagcgtc agcggcccag tcgcccaggc tggcgctgcg atctcccagc
     1621 atctggacga gttcggcgcc ccccccgaga agcagagctg cgcgcgccag gtcccagcgc
     1681 cgcatgcgcg ggaaggccgc gaacgaaacc gccgtgagcc cgccgaaagc gatggcgtgc
     1741 gcgctcttgt cattcaggcc gaaggccgcc tccgcgcctt gaaacggacc cagcatcaag
     1801 atcgcggccg tcagggcgcc cagcagaagc acggcgcggg cggtgatcac gatgtgattg
     1861 ggcgtaagca tggggcaggc tctcggtgag gcctttccat aacgagcgcc tcgccgccat
     1921 ctagtgaacg aacaagatca accgaggatg aatcaggtca tcctcggtcg actgccgcgg
     1981 acctctagcg cttccgggcc ttggctttcg ccttggccct ggcggcctcc tctgccttct
     2041 tcttggcgtc agcttccgcc ttggcagcgt cgtccttggc ctttttccag acctgatagg
     2101 cttcgatatg aggctggcgg cgcaggatct tcgaggccgg gtcgacgatc accggagcag
     2161 ccgttccaat ccgcacgcgg ccggaaccgt ctgccatcgg cagggcgacc atcctgccgc
     2221 cgaccttgac ctcgacgggc atggggaaag gcttgtcctt cggcgttttc cagcgaagca
     2281 tcagatcgtc gccttcgcgg ctttccatca gttccggcgg gctggcttcg tacagataga
     2341 cgtcgaagaa ccagccatag tcctggccgg tcacatcatt caccgcgcga atgaaatccc
     2401 gggtgttggc gtagcgcggc gcgaaattgc ctggcttggg atccgttcga ccatagacca
     2461 caaggcggat cgccttgaag aaggcctcgt cgccgatcag gctccgcagc gtgtgcaggg
     2521 cgttcgagcc cttgtagtag atgtcgttgc cggggccgac cttgggatca tagacctgat
     2581 cctcggccat cgacttgccg gccaccaccg ggaacttgtt ctgcaaggtg gcgcgctggg
     2641 tgttgaggcg ggccatgtat tccatgtcgc cgtgcagcca ctgcgaatag agcggctgca
     2701 tgtaggtgcc caggccctcg tgcagccaca tgtcgtccca gtcgacattg gtcagctggt
     2761 tgccgaacca ctcgtgcgcc agttcgtggt gcagcagcca gtcatagccg tagacgtcct
     2821 tggcgtactc gttgccatag gcgttgatgg tctggtgctc catgcccaga tgcggggtct
     2881 cgaccacgcc catcttctcg tcgcggaacg ggtaggggcc gatcatggcc tcgtagaagt
     2941 cgagcatcgg cccgaactcg gcgaacagcg ccttggcctc ggtctcgcgg cccttcaggt
     3001 gccagaacct gatcgggaag ctgtcgccat aacggctatt gtaggtgtca ctcagctcga
     3061 cataggggcc gatgtcgaga gcgatggcat aggtgttggg gttcttggcg cgccagctgt
     3121 aggtccgcca gccatccttc tcctccatgc cgacgaacac cccgttagcc ggcgcgacca
     3181 gcggggctgg gacgacgatg tgcaggtcga ccagcttggg ctcgccggtg ggctgatcga
     3241 tacagggcca gaaaaggtcg caaccgtcgc cttgcacggc ggtggcgatc cacggctcgc
     3301 cggtcttggt cttcttccag acgaagccgc cgtcccaggg ggggcgtttg gcttcatgcg
     3361 gtacgccggc atactgaatg cgaaccttga cctgctcacc cttggccagc ggcatgggca
     3421 gggtgatcga caggcgacct tcgggattgc tccaggcgct ggcggcgagc ggcgcgccat
     3481 cgatcgtcag caagctgatg gggagatccc gatcaaggtc caaaatcagc ctggtcagcg
     3541 gggcccgggc cagaatggtc agggtcgcgt cgccctcgat agccttgcgc tccgggatca
     3601 ccttgatgcg cagatcgaca tgctccagcg acatgtcgcg ttgttcggcg ggggccacgc
     3661 cgcctgtgct cagggtcagg gcggtggtct ttttggccgg ggccttggtt tgggcaaggg
     3721 cctggccggc gccgagcgcc agcaggcaaa ccgcactggc ggcggcgagg gtggaacgag
     3781 gcaaacggga ctccccaggg cggcggttct ttgcgccatg cgtaggcccg ggcggcgcga
     3841 acgccaagag ggtcagattt tctcgctgat cttgatcgcg gcgcgacagc ccgcctggat
     3901 cacggcattg ag
//
LOCUS       DAOMEO010000160         3905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2734_length_3905_cov_3.105974, whole genome shotgun sequence.
ACCESSION   DAOMEO010000160 DAOMEO010000000
VERSION     DAOMEO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2734_length_3905_cov_3.105974"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLH31_04905"
     CDS             <1..265
                     /locus_tag="PLH31_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HQR88599.1"
                     /translation="ASATLAAPASKATLYETTGKIERITANSVTLSHEPVPALDWPAM
                     TMTFALADPGIARGFKAGDRVRFGFDRPPAGPTLRHMAKVAGR"
     gene            262..3405
                     /locus_tag="PLH31_04910"
     CDS             262..3405
                     /locus_tag="PLH31_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88600.1"
                     /translation="MIAHLIRWSVRNRFFVLIGMLALIGAGLWAVRSTPIDALPDLSD
                     VQVVIRTSYPGQAPQIVESQVTYPLTTTMLSVPGAKTVRGYSFFGDSFVYVIFEDGTD
                     LYWARSRVLEYLNQVQGRLPANARSALGPDATGVGWVYEYALVDRTGRHDLSQLRGLQ
                     DWFLRYELKTVTGVAEVASIGGMVRQYQILLDPVKLAAYGVTHAQAVEAIRQANQEAG
                     GSVLELAEAEYMVRASGYLKTLDNFGAIPLKTAAGGVPVRLGDVATIQVGPEMRRGIG
                     ELNGQGEVAGGVVILRSGKNARETIAAVKDKLADLRKSLPPGVEVVTVYDRSQLIDRA
                     VENLTHKLVEEFIVVALVCGLFLWHVRSALVAILTLPLGVLAAFVVMRYQGVNANIMS
                     LGGIAIAIGAMVDAAVVMIENAHKKIERWEQDHPDTHLDRETRWIVVTEAAAEVGPAL
                     FFSLLIITLSFIPVFTLEAQEGQLFAPLAFTKTYAMGAAAILSVTLVPILMGWLIRGR
                     IPAEQANPVNRWLTDLYRPAIDWTMRRPKAVLLIATLVFATTAWPLSRLGGEFMPSLD
                     EGDLLYMPSALPGLSAAKASELLQQTDRLIKTVPEVETVFGKAGRAETATDPAPLEMF
                     ETTIQFKPRDQWRPGMTPEKLVDELDRTVKLPGLANIWVPPIRNRIDMLATGIKSPIG
                     LKVSGSDLAELDRIAHDVETVARTVPGVSSALAERLTGGRYVDVDIDRAAAARFGLNI
                     ADVQAIVSGAIGGETIGETVEGRARYPISVRYPRELRDSLEGLRALPILTPAGQQITL
                     GTVANVSIAEGPPMLKTENARPSTWVYVDVRGRDLASVVGDLQRAVAKQVRLSPGVSI
                     AYSGQFEYLERAVDRLKLVVPATLLIIFVLLYLIFGRFDEAALIMGTLPFALTGGIWT
                     LYLLGFNQSVATGVGFIALAGVSAEFGVVMLIYLKNALAEHTANPDAAEVEAAVREGA
                     LLRVRPKAMTVAVILAGLLPILLGSGAGSEVMSRIAAPMIGSMLTAPLLSMLVLPAAY
                     LLLRRPKTDPIPQPASS"
     gene            3402..3806
                     /locus_tag="PLH31_04915"
     CDS             3402..3806
                     /locus_tag="PLH31_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HQR88601.1"
                     /translation="MKEKTMNHVRLTIALGLAALTAACGKKAEAPVATETNEAAHAAT
                     MSGDMGNMSMAPDANAAIKAKGHGTVTAIDKAAGTITLDHGPIPEAKWPAMTMAFKAA
                     PSITDAVKVGDKVDFDLSLKGSDGEVTAIAKQ"
BASE COUNT          632 a         1306 c         1314 g          653 t
ORIGIN      
        1 ggcatcggcg accctcgccg cgccggcgtc gaaagccacg ctgtacgaga cgacgggcaa
       61 gatcgagcgg atcacggcca attcggtgac gctcagccac gagccggtcc ccgcgctcga
      121 ctggccagcg atgacgatga ccttcgcact cgccgatccg ggcatcgcgc gcggcttcaa
      181 ggcgggtgac cgggttcggt tcgggttcga ccggcccccg gcgggaccga cgctgcggca
      241 tatggcgaag gtggcgggcc gatgatcgcc catctcatcc gctggtcggt caggaaccgc
      301 ttcttcgttc tgatcgggat gctggcgctg atcggcgcgg gcctgtgggc ggtgcgctcg
      361 accccgatcg atgccctccc cgatctctcg gacgtgcagg tcgtgatccg caccagctat
      421 cccggccagg cgccgcagat cgtcgagagc caggtcacct atccgctcac caccaccatg
      481 ttgtcggtgc ccggggcaaa gaccgtgcgg ggctacagct tcttcggcga cagcttcgtc
      541 tatgtgatct tcgaggatgg caccgatctc tattgggcgc gcagccgcgt gctcgaatat
      601 ctgaaccagg tgcaggggcg cctgccggcg aacgcccgca gcgcgctcgg tcccgacgcg
      661 accggggttg gctgggtcta tgaatatgcg ctggtcgacc gcaccggccg gcatgacctg
      721 tcgcagctgc gcgggctgca ggactggttc ctgcgctatg aactcaagac cgtgaccggc
      781 gtcgccgagg tcgccagcat cggcggcatg gtcaggcagt atcagatact gctcgatccg
      841 gtgaagctcg ccgcctacgg ggtgacccac gcccaggcgg tcgaggcgat ccggcaggcc
      901 aatcaggaag cgggcggctc ggtgctcgag ctggccgaag ccgaatatat ggttcgcgcc
      961 tcgggctatc tgaagacgct cgacaatttc ggcgcgatcc cgctgaagac ggcggccggt
     1021 ggcgtgccgg tccgtctcgg tgacgtcgcc acgatccagg tcggtcccga aatgcgacgc
     1081 ggcatcggcg aactgaacgg ccagggtgag gtcgccggcg gcgtcgtgat cctgcgttcg
     1141 ggcaagaatg cccgcgagac catcgcggcg gtcaaggaca agctcgccga tctcaggaag
     1201 agcctgccgc ccggcgtcga ggtggtgacg gtctatgatc gctcgcaact gatcgatcgc
     1261 gcggtcgaga acctcaccca caagctggtc gaggagttca tcgtcgtcgc cctggtctgc
     1321 ggcctgttcc tctggcatgt ccgctcggcg ctggtcgcga tcctgacgct gcccttgggc
     1381 gtgctcgccg ccttcgtcgt gatgcgctac cagggcgtca acgccaacat catgtcgctg
     1441 ggcggcatcg ccatcgcgat cggcgcgatg gtcgatgcgg ccgtggtcat gatcgagaac
     1501 gcccacaaga agatcgagcg ctgggaacag gaccatccgg acacccatct cgaccgcgag
     1561 acgcgctgga tcgtcgtcac cgaggcggcg gccgaggtcg ggcccgcgct gttcttcagc
     1621 ctgctgatca tcacgctgtc cttcatcccg gtgttcacgc tggaagccca ggaaggccag
     1681 ctgttcgcgc cgctcgcctt caccaagacc tatgcgatgg gcgccgccgc gatcctgtcg
     1741 gtgacattgg tgccgatcct gatgggctgg ctgatccggg gacgtattcc ggccgagcag
     1801 gccaacccag tcaaccgctg gctgaccgat ctctaccggc ccgcgatcga ctggacgatg
     1861 cggcggccca aggcggtgct gctgatcgcg acgctggtat tcgcgaccac ggcctggccg
     1921 ctgtcgcggc tcggcggcga gttcatgccc agcctcgacg agggcgacct actctacatg
     1981 ccctcggcat tgcccggcct ctcggcggct aaggcgagcg agctgcttca gcagaccgac
     2041 cggctgatca agaccgtgcc cgaggtcgag accgtgttcg gcaaggcggg tcgcgccgag
     2101 actgcgaccg atcccgcgcc actcgaaatg ttcgaaacaa cgatccagtt caagccgcgc
     2161 gaccaatggc ggccgggtat gacgcctgag aagctggtcg acgagctcga ccgcacggtg
     2221 aagctcccgg gcctcgccaa tatctgggtg ccgccgatcc gcaaccggat cgacatgctg
     2281 gcgaccggca tcaagagccc gatcgggctc aaggtgtcgg gcagtgatct cgccgagctc
     2341 gaccggatcg cccatgatgt cgagacggtg gccaggaccg tgccgggcgt cagctcggcg
     2401 cttgccgagc ggctgaccgg cggacgctat gtcgatgtcg acatcgaccg cgccgccgcc
     2461 gcgcggttcg ggctcaacat cgccgacgtc caggccatcg tctcgggcgc gatcggcggc
     2521 gagacgatcg gcgagacggt ggagggccgc gcccgctatc cgatcagcgt gcgctatccg
     2581 cgcgaattgc gcgacagcct cgaagggctg cgggcgctac cgatcctgac gcccgcgggc
     2641 cagcagatca ccttgggcac cgtggcaaac gtctcgatcg ccgagggccc gccgatgctc
     2701 aagaccgaga atgcccggcc ttcgacctgg gtctatgtcg atgtgcgcgg gcgcgatctc
     2761 gcctcggtgg tcggcgatct gcagcgtgcg gtggcgaaac aggtcaggct ctcgccgggc
     2821 gtcagcatcg cctattcggg ccagttcgaa tatctcgagc gcgcagtcga ccggttgaag
     2881 ctggtcgtgc ccgcgacgct gctgatcatc ttcgtgctgc tctacctgat cttcggccgc
     2941 ttcgacgagg ctgcgctgat catgggcacg ctgccgttcg cgctgaccgg cggcatctgg
     3001 acgctctatc tgctggggtt caaccagtcg gttgccaccg gggtcgggtt catcgcgctc
     3061 gccggcgtgt ccgccgagtt cggcgtggtg atgctgatct atctcaagaa cgcgctggcg
     3121 gagcacaccg caaatccgga cgccgccgaa gtcgaggccg ccgtgcgcga aggcgcgctg
     3181 ctgcgcgtcc ggcccaaggc catgacggtc gcggtgatcc tcgccggcct gctgccgatc
     3241 ctgctgggat cgggtgcagg ctcggaggtc atgagccgga tcgcggcacc gatgatcggc
     3301 agcatgctga ccgcgccctt gctctccatg ttggtgctgc ccgccgccta cctgctgttg
     3361 cgccggccca agaccgatcc catccctcaa cccgcttcat catgaaggag aagacgatga
     3421 accatgtacg actgaccatt gccctcggcc tcgccgccct gactgccgcg tgcggcaaga
     3481 aggcggaggc ccccgttgcg actgagacca acgaggcggc gcacgccgcg accatgagcg
     3541 gcgacatggg caacatgtcg atggcgcctg acgcgaacgc cgcgatcaag gccaagggcc
     3601 atggcaccgt caccgcgatc gacaaggcgg caggcacgat cacgctcgat catggtccga
     3661 tcccggaggc caagtggccg gcgatgacga tggcgttcaa ggccgcgccg tcgatcaccg
     3721 atgcggtcaa ggtcggcgac aaggtcgatt tcgacctctc gctcaagggc agcgatggcg
     3781 aggtcaccgc gatcgcaaag cagtgatccg ctaagttcga ccggaaagag ctggttgcgt
     3841 cgccgctccc gggcggcgac gcccgcgccg gccttctgcc tgcctgtgcg cccggtatac
     3901 ggatg
//
LOCUS       DAOMEO010000161         3905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2735_length_3905_cov_3.019740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000161 DAOMEO010000000
VERSION     DAOMEO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_3905_cov_3.019740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLH31_04920"
     CDS             complement(<1..545)
                     /locus_tag="PLH31_04920"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQR88602.1"
                     /translation="MSDGNAGKRLRSSGSYGKLDRDGFIHRSWMKSQGLPDDVFDGRP
                     VIGICNTWSEITPCNAGLRDIAEHVKRGVWEAGGLPLEFPAISLGETQMRPTAMLFRN
                     LLAMDVEESIRGNPIDGVVLLGGCDKTTPGQMMGAASVDLPTIVISSGPMLNGKFRGK
                     DIGSGTDVWKFSEAVRAGEMTL"
     gene            761..>3256
                     /locus_tag="PLH31_04925"
     CDS             761..>3256
                     /locus_tag="PLH31_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQR88603.1"
                     /translation="MTGLNTLRAALCGLALMASTSALALEAGFEKTVDGVVVTPASGP
                     AKKVRLQVMGDRIIHVTATPTDSLAIPPSLMVTARPATAGFTVTASGDSVVLKTAKTS
                     ATISLQDGTVGFRDAAGKLVLSERDRGAFIPVKVEGQDFYEVRQQFNPGTDEAFYGLG
                     QHQNGQMNYNGQDVQLLQYNRVVAVPMLLSSKGYGLLWDNNAITRFGNPVPYDLASRD
                     LKIFDAEGKPGGFTARYYVGGKLKVSRVEKDIDYRFLKDLPGWPAEAVDPATKAPMRD
                     QKVVWEGQIQTDKAGVHTFQLYSSDYAKVFVDDQLVIDRWRQNWNPWYHNFSAALTPG
                     RKHKVRVEWNPDGGYIGLLHNDPMAKADRHSLYLTSEVGKAIDYYFVGGDDLDQVISG
                     YRDLTGAAVMMPRWAYGFWQSRQRYETAEQIVGVVKEYRRRKLPLDNIVMDWRYWKDP
                     EWGTHRFDETRFPDPKAMVDEIHGLNAHFMISIWPKFYPTTDNYKELDAKGFMYRRNV
                     EQGAIDWVGPGYKNSFYDPYPAEARAIYWRQVKDNLKALGVDAWWMDASEPDLHSNLD
                     VAERTLRMSPTALGPGAEYFNSYSLVHTQGVYEGERALNPDVRPFILTRSGFGGLQRH
                     ASAVWSGDVVARWDDFRDQISAGVNLSMSGIPNWTTDIGGFSVEARYTNKDPAHWAEW
                     QELNLRWFQFGAFSPLFRSHGEFPLREIYNLADEGTEVYQALAWYNELRYRLMPYVYT
                     LAAETYHRDGSLMRGLVMDFPDDRKVRNIDDQYLFGREFLVAPVTSFKARSRDVYLPA
                     GATWYDFETGQRHAGGQTIKAAAPLSRMPLFVKA"
     assembly_gap    3257..3266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3267..3618
                     /locus_tag="PLH31_04930"
     CDS             <3267..3618
                     /locus_tag="PLH31_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HQR88604.1"
                     /translation="GSIVPTTVVQQYVGEKPDAPITLVVYTGKDGRFELYEDDGLSNG
                     YERGAYSRIPIAYDDASGRLTIGARSGSFKGMLDRRVIKVRFIGDGARPTDFEMVDAS
                     VDYVGQPVVLKRKR"
BASE COUNT          698 a         1294 c         1247 g          656 t
ORIGIN      
        1 agggtcattt cgccggcccg gacggcctcg gagaacttcc acacgtcggt gcccgagccg
       61 atgtccttgc cccggaactt gccgttcagc atcgggcccg agctgatcac gatggtgggc
      121 aggtcgacgc tcgccgcgcc catcatctgg cccggggtgg tcttgtcgca accgcccagc
      181 agcacgacgc cgtcgatcgg attgccgcgg atcgattcct cgacatccat ggccagcagg
      241 ttgcggaaca gcatggccgt gggtcgcatc tgggtctcgc ccaggctgat ggccgggaac
      301 tccagcggca ggccgccggc ttcccagacg ccgcgtttca cgtgctcggc gatgtcgcgc
      361 aggccggcgt tgcagggggt gatctcggac caggtgttgc agatgccgat cacgggacgg
      421 ccgtcgaaca cgtcgtccgg cagaccctgg ctcttcatcc agctgcgatg gatgaaacca
      481 tcgcgatcca gcttgccgta ggacccgctg ctgcgcaggc gcttgcccgc gttaccgtca
      541 ctcataacca tccctatcgc cggctggatc cggctctcga acgcctcttt cggccattta
      601 tagtattata gggattatcg caaaccctgc cgttggcgcc gcgcgaaggt tgcttttcaa
      661 gcggcgtccg ggccatcatc agccatagct ggtagcgcta acaggatcgc gatcgccgtt
      721 tcaacggatg gcgcgaggct caccggcggg ggaagaaacg atgaccgggc tcaacaccct
      781 tcgcgccgcc ctgtgcggcc tggccctgat ggcctcgacc tcggccctgg cgctggaggc
      841 ggggttcgag aagaccgtcg acggcgtggt cgtcaccccc gcctcgggac cggccaagaa
      901 ggtgcggctg caggtgatgg gcgaccgcat catccacgtc acggccacgc ccaccgacag
      961 tctggcgatc ccgcccagcc tgatggtgac ggccagaccg gcgacggcgg gcttcaccgt
     1021 caccgcctcg ggcgacagcg tggtgctgaa gaccgccaag acctcggcca cgatctcgct
     1081 gcaggacggc acggtcggct tccgcgacgc cgccggcaaa ctggtgctca gcgaacgcga
     1141 ccgcggcgcc ttcatcccgg tgaaggtgga ggggcaggac ttctacgagg tccgccagca
     1201 gttcaatccc ggcacggacg aggccttcta tggcctgggc cagcaccaga acggccagat
     1261 gaactacaac ggccaggacg tgcagctgct gcagtataac cgcgttgtcg cggtgccgat
     1321 gctgctgtcc agcaagggct atggcctgct gtgggacaac aatgcgatca cgcggttcgg
     1381 caacccggtc ccctatgacc tggcctcgcg cgacctgaag atcttcgacg ccgagggcaa
     1441 gcccggcggc ttcacggccc gctactatgt cggcggcaag ctcaaggtca gccgggtcga
     1501 gaaggacatc gactatcgct tcctgaagga cctgccgggc tggccagccg aagcggtcga
     1561 tccggcgacc aaggccccga tgcgcgacca gaaggtggtt tgggaaggtc agatccagac
     1621 cgacaaggcc ggcgtccaca ccttccagct ctattccagc gactacgcca aggtgttcgt
     1681 cgacgaccag ctggtgatcg accgctggcg gcagaactgg aacccctggt accacaactt
     1741 cagcgccgcc ctgacgccgg gcaggaagca caaggtccgc gtcgagtgga acccggacgg
     1801 cggctatatc ggcctgctgc acaatgatcc gatggccaag gccgaccgcc actcgctgta
     1861 cctgacctcc gaggtcggca aggcgatcga ctactatttc gtgggcggag atgacctgga
     1921 tcaggtcatc tcgggctatc gcgacctgac cggcgcggcg gtgatgatgc cgcgctgggc
     1981 ctacggcttc tggcagagcc gccagcgcta tgagaccgcc gagcagatcg tcggggtggt
     2041 caaggagtac cgcaggcgca agctgccgct cgacaacatc gtgatggact ggcgctactg
     2101 gaaagatccc gagtggggca cccataggtt cgacgagacg cgctttcccg accccaaggc
     2161 catggtcgac gagatccacg gcctgaacgc ccacttcatg atctcgatct ggcccaagtt
     2221 ctatccgacg acggacaact acaaggagct ggacgccaag ggcttcatgt accgccgcaa
     2281 tgtcgagcag ggcgcgatcg actgggtggg gccgggctac aagaactcgt tctatgaccc
     2341 ctatcccgcc gaggcccgcg ccatctactg gcgtcaggtg aaggacaatc tgaaggccct
     2401 gggcgtcgac gcctggtgga tggatgcgtc agagccggac ctgcactcca atctcgacgt
     2461 ggccgagcgc accctgcgca tgagccccac ggcgctgggc cccggcgccg aatacttcaa
     2521 cagctattcg ctggttcaca cccagggcgt ctatgagggc gagcgcgccc tgaaccccga
     2581 cgtgcggccg ttcatcctca cccgctcggg ctttggcggt ctgcagcgcc acgcctcggc
     2641 ggtgtggagc ggcgacgtgg tggcgcgctg ggacgacttc cgcgaccaga tctcggcggg
     2701 ggtgaatcta tcgatgtcgg gcattcccaa ctggaccacc gacatcggcg gcttctcggt
     2761 cgaggctcgc tacaccaaca aggaccccgc ccattgggcc gagtggcagg agctgaacct
     2821 gcgctggttc cagtttggcg ccttcagccc gctgttccgc agccacggcg agtttccgct
     2881 ccgcgagatc tacaacctcg ccgacgaggg cacggaagtt taccaggccc ttgcctggta
     2941 caacgagctt cgctaccggc tgatgcccta tgtctacacc ctggcggccg agacctatca
     3001 ccgcgacggc agcctgatgc gcggtctggt catggatttc ccggacgacc ggaaggtccg
     3061 caacatcgac gaccagtacc tgttcggccg cgaattcctg gtcgccccgg tgaccagctt
     3121 caaggcccgc tcgcgcgacg tctacctgcc ggccggcgcg acctggtacg acttcgagac
     3181 cggccagcgc cacgccggcg gccagaccat caaggccgcc gcgcccctgt cgcgcatgcc
     3241 gctgttcgtg aaggccnnnn nnnnnncggc tccatcgtgc cgaccacggt ggtgcagcaa
     3301 tatgtcggcg agaagccgga cgcgcctatc accctggtcg tctacaccgg caaggacggc
     3361 aggttcgagc tctacgagga tgacggcctg agcaacggct acgagcgcgg cgcctatagc
     3421 cgcattccga tcgcctatga cgacgccagc ggccgcctga cgatcggcgc gcggtcgggg
     3481 tcgttcaagg gaatgctgga ccggcgcgtc atcaaggttc gcttcatcgg tgacggcgca
     3541 aggccgaccg acttcgagat ggtcgatgcc agcgtggact atgtcgggca gccggtggtg
     3601 ctgaagcgca agcgataggt cgctgcgcta gatagccgag gggtcttctc gccccgacac
     3661 gtcagatgcg cctaggccgg cggggctgcc gggccagggc ggtggagcgt ctgcccaccc
     3721 acgccgcctt catcgcccag cactgccggg cccccgactg aacacggcga ttccggtaaa
     3781 ggctccgcta acggacccgg tgcgccgagt ggcaaaaggc gtgtttgagt ctaatcagac
     3841 tcagacttaa cgagttttca ctatacagtt ttccgaactg ttatgattaa cgaagttgtt
     3901 ccatg
//
LOCUS       DAOMEO010000162         3904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2736_length_3904_cov_2.898935, whole genome shotgun sequence.
ACCESSION   DAOMEO010000162 DAOMEO010000000
VERSION     DAOMEO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_3904_cov_2.898935"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PLH31_04935"
     CDS             <1..100
                     /locus_tag="PLH31_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HQR88605.1"
                     /translation="LAAWADFGLDTSKTRGSFKAAIPKPNLLKFPG"
     gene            complement(117..1565)
                     /locus_tag="PLH31_04940"
     CDS             complement(117..1565)
                     /locus_tag="PLH31_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88606.1"
                     /translation="MTHASLNRRAVLAAVGLVLPGLALAQPAAPISQAGPFRFKGWKG
                     QDTDAERGFFEVPEDRRDPKSRKIRLSYVRFASTAARPGPPIIYLAGGPGGTATRALA
                     GPRFPIMMALREVADVIAFDQRGTGLSNHIPERPVSTRPLPELTQAGLTAYFREDFKS
                     AWADWTRAGVAMTGYNTEQNADDIDDLRRHLGAEKVDLWGISYGTHLALSMLKRHGDR
                     VNRVALASLEGQDQTVKRPAAVDALLQQVDGLLAADPAIRAAIPDLPALMRRVHARME
                     AAPVKMPAMLNGAPVELKLGGFALQLMAGGLIANPQTLVMLPRLYLALDAGRTEVLTP
                     FMGEFANIPGVAGMPEATDLASGISPARLALVRREAKTAVLGEALNFPMPQLLGVVPG
                     VDLGENFRAPLRIDHPALLIAGTLDGRTPMSEQDEVAAQFTRKSRVIVENAGHNVFEA
                     HPDVQGLLVRFFRGEAVADARLSLPPPTFGLG"
     gene            1677..3098
                     /locus_tag="PLH31_04945"
     CDS             1677..3098
                     /locus_tag="PLH31_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQR88607.1"
                     /translation="MWRILILAVVLTGLSTLARAKDMAWEACRGAQGPAGPVLNDCRP
                     VRDFVDPQGRDLWIRSTIRTPGDTRPRALYVAGVASSQAWFNGQSLGANGRPGATALE
                     EVPGRYQVVFPIRETLWRAGGNRLVLRLSSFHGGLRFARPMSAMVVLPYPYPQRTALL
                     AVTFVTAGALLAAAFGFGVIHALRRTLSSLLLAGMAGVAALQAVVESLRPLFDYPYPF
                     HAWRMSAIWALAAAFAILLVVSVASRFLPRARGLMTGLALGVVGATVLLPGFDIKTAW
                     ALILGVALAVPPAAAGVRLQVPGARLTLAYLGLFLAVALGFPEWLADFSFFLLAASLV
                     LPLLMVEVVRLGRDDRSREAALTQAVSRPDRLTVASARGVELAPIKEILAVVGADDYV
                     ELRLVGGRSLLHAARLDGLSSQLPENFLRVHRSVIANLAHVQRLERDGDRWRLHLSEG
                     APLPVSRSRQPALREALEDPGRP"
     gene            complement(3095..>3904)
                     /gene="prfC"
                     /locus_tag="PLH31_04950"
     CDS             complement(3095..>3904)
                     /gene="prfC"
                     /locus_tag="PLH31_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672038.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQR88608.1"
                     /translation="GELLAGIGAYAPAPHPAKAVRGGAETHVAPGDSEVSGFVFKVQA
                     NMDPNHRDRIAFLRLTSGRFTRGMKLKAQNTGKSMSVNAPVMFFASDRELAEDAYAGD
                     VIGIPNHGVLRVGDSLSESGLLRFAGLPNFAPEILRRVRVKDPLKAKHLKKALEGLAE
                     EGVTQLFRPMLGSSDFIVGAVGQLQFEVMADRLGGEYALDVIFEPSPYAEARWISGAR
                     ADVEDFQDKHKTAMATDIDDQPVFLGKSSWEIGYVAERFPKVRFERTRERG"
BASE COUNT          553 a         1374 c         1342 g          635 t
ORIGIN      
        1 tctcgccgcc tgggccgatt tcgggctcga caccagcaag acacgcggaa gcttcaaggc
       61 cgcgatcccc aagcccaacc tgctgaagtt tccgggttag agcccctccc cgtcgctcaa
      121 cccaacccga acgtcggcgg cggcaggctc agccgcgcgt cggcaacggc ctcgccgcgg
      181 aagaaccgca cgagcagccc ctgcacatcg gggtgggcct cgaagacatt gtggccggcg
      241 ttctcgacga tcactcggga tttgcgggtg aactgggcgg ccacctcgtc ctgctcggac
      301 atcggcgtgc ggccgtccag cgtgcccgcg atcaacagag ccggatggtc gatgcgcaac
      361 ggcgcccgga agttctcgcc cagatcaacc ccaggaacca cgcccagcag ctgaggcatc
      421 gggaagttca gcgcctcgcc cagcacggcg gtcttggcct cgcgccgcac cagggccagt
      481 cgggcgggcg agatccccga cgccagatcc gtggcctcgg gcatgccggc gacgccggga
      541 atgttggcga attcgcccat gaacggcgtc agcacctctg tcctgcccgc atcgagcgcc
      601 agatagagtc gcggcagcat gaccagggtc tgcggattgg cgatcagccc gccggccatc
      661 agctgaaggg cgaagccgcc cagcttcagc tcgaccggcg cgccgttcag catcgcaggc
      721 atcttgaccg gcgcagcctc catcctggca tgcacccgcc gcatcagggc cggcaggtcg
      781 gggatcgcgg cgcggatggc gggatcggcg gccagcaggc cgtccacctg ctgcaggagg
      841 gcgtcaaccg ccgccggccg cttcacggtc tggtcctggc cctcaagact ggccagggcc
      901 acgcggttca cccggtcgcc gtgccgcttg agcatgctca gcgccaggtg cgtgccgtag
      961 ctgatgcccc acagatcgac cttctccgcg cccaggtgac gccgcaggtc gtcgatgtca
     1021 tcggcgttct gttcggtatt gtagccggtc atggccacgc cggccctggt ccagtcggcc
     1081 caggcgctct tgaagtcctc gcggaaatag gcggtcagcc cggcctgggt aagctctggc
     1141 aacgggcgcg tcgagaccgg tcgctcaggg atgtggttgg acaggcccgt accgcgctgg
     1201 tcgaaggcga tgacgtcggc cacctcgcgc agggccatca tgatcggaaa ccgcggtccg
     1261 gccagcgcac gggtcgccgt gccgcccggt ccgccagcca gatagatgat cggcggcccc
     1321 ggcctggccg ccgtcgaggc gaaccgcaca tagctcagcc ggatcttccg ggacttggga
     1381 tcgcggcgat cctccgggac ctcgaagaag ccgcgttcgg cgtcggtgtc ctggcccttc
     1441 cagcccttga agcggaatgg cccggcctgt gatatcggcg cggcaggctg tgcgagcgcc
     1501 agaccaggaa ggacgaggcc gacggcggca aggacggcgc ggcgattgag tgaggcatgg
     1561 gtcatggcga caacagagcc ggccggcacg tcgctcgcca cgccggttcg gtcaacggcg
     1621 tcccctcatc ggcgaacggt ggcgcgcccc ggcgaacggc gccttaaggt gaaggcatgt
     1681 ggcgcatcct gatccttgcc gtcgtcctga ccggcctttc gaccctggcg cgggccaagg
     1741 acatggcctg ggaggcctgc cgcggcgcgc agggtccagc gggccctgta ctgaacgact
     1801 gccggccggt gcgggacttc gtcgacccgc agggtcgcga cctttggatc cgctccacga
     1861 tcaggacgcc cggcgacaca cggccccgcg cgctctatgt ggccggcgtc gcgtcgtcac
     1921 aggcctggtt caacggccag agcctgggcg ccaatggccg ccccggggcc accgcgcttg
     1981 aagaggttcc cggacgctat caggtggtgt ttcccatccg agagactctt tggcgagccg
     2041 gcggcaaccg gctggtgctg cgcctgtcgt cgttccacgg cggcctgcgc tttgcccgcc
     2101 cgatgagcgc catggtcgtc ctgccctatc cctatccgca acgcaccgcc ctgctggcgg
     2161 tcaccttcgt gactgcgggc gccctgctgg cggcggcgtt cggcttcggc gtgatccacg
     2221 ccctgcggcg caccctgtcg agcctgttgc tggccggcat ggccggggtt gcggccctgc
     2281 aggccgtcgt cgagagcctg aggccgctgt tcgactatcc ctatccgttc cacgcctggc
     2341 ggatgagcgc catctgggcc ctggcggccg cttttgcgat cctgctggtg gtctccgtcg
     2401 ccagccggtt cctgcccagg gcgcgcggtc tgatgaccgg actggccctc ggcgtggtcg
     2461 gcgccacggt gctgttgccg ggcttcgata tcaagacggc ctgggcgttg atcctgggtg
     2521 tcgcactcgc cgtgccgccc gccgcagcgg gcgtgcgcct gcaggttccc ggcgcgcgac
     2581 tgaccctggc ctatctgggg ctgttcctcg ccgtggcgct gggctttcct gagtggctgg
     2641 ccgacttctc ctttttcctg ctcgccgcca gtctggtgct gccgctgctg atggtcgagg
     2701 tggtgcggct gggccgcgac gaccgcagcc gcgaggccgc cctgacccag gccgtcagcc
     2761 gtccggatcg cctgaccgtc gcctcggcgc gcggcgtcga gctggcgccg ataaaggaga
     2821 ttctggccgt ggtgggggcc gacgactatg tcgaactaag gctggtcggc ggccgcagcc
     2881 tgttgcacgc cgcgcgcctg gacggtctgt ccagccagct tccggagaac ttcctgcgcg
     2941 tccaccgctc ggtgatcgcc aacctggcgc acgtccagcg gctggagcgg gacggcgacc
     3001 gctggcggct gcatctgagc gagggcgcgc ccctgcccgt cagccgctca cgccagccgg
     3061 ccctgcgcga agctctggaa gaccctggaa gaccctagcc ccgctcgcgc gtccgctcga
     3121 accgcacctt ggggaagcgt tcggccacat agccgatctc ccaggacgac ttgcccagga
     3181 acaccggctg gtcgtcgatg tcggtggcca tggccgtctt gtgcttgtcc tggaagtcct
     3241 cgacgtcggc gcgggcgccg ctgatccagc gggcctcggc atagggggag ggctcgaaga
     3301 tcacgtccag ggcatattcg ccgcccagcc ggtcggccat gacctcgaac tgaagctggc
     3361 ccacggcgcc gacgatgaag tcggagctgc ccagcatggg gcggaacagc tgggtgacgc
     3421 cttcctcggc caggccttcc agcgccttct tcaggtgctt ggccttcagc ggatccttga
     3481 cccgcacgcg gcgcaggatt tccggggcga agttgggcag gccggcaaag cgcagcagtc
     3541 cgctttccga caggctgtcg cccacccgca gcacgccatg gttgggaatg ccgatcacgt
     3601 cgccggcata ggcgtcctcg gccagttcgc ggtctgacgc aaagaacatc accggcgcat
     3661 tgacgctcat cgacttgccg gtgttctggg ccttcagctt catgccgcgc gtgaaccggc
     3721 cgctggtcag gcgcaggaag gcgatccggt cgcggtgatt ggggtccata ttggcctgga
     3781 ccttgaagac gaagcccgag acctcgctgt cgccgggcgc gacatgggtc tcggcgccgc
     3841 cccgcacggc cttggccgga tgcggcgcag gcgcataggc gccgataccg gccagcagct
     3901 cacc
//
LOCUS       DAOMEO010000163         3865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2753_length_3865_cov_2.570079, whole genome shotgun sequence.
ACCESSION   DAOMEO010000163 DAOMEO010000000
VERSION     DAOMEO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_3865_cov_2.570079"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..439
                     /locus_tag="PLH31_04955"
     CDS             62..439
                     /locus_tag="PLH31_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88609.1"
                     /translation="MTKKVLIVEDNELNMKLFHDLLEAQGYETLQTREGLQALSIARD
                     NRPDLILMDIQLPEISGLEVTKWLKEDEDLAHIPVVAVTAFAMKGDEERIREGGCEAY
                     ISKPISVAHFLDTIKRLLEKTPA"
     gene            436..1800
                     /locus_tag="PLH31_04960"
     CDS             436..1800
                     /locus_tag="PLH31_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HQR88610.1"
                     /translation="MSARILVVDDIEANVRLLEAKLSAEYYQVSTASDGPTALAMAAN
                     DLPDIILLDVMMPGMDGFTVCKRLKDDPITRHIPVVLVTALDGRADRIQGLEAGASDF
                     LTKPIDDVMLFARVRSLTRLKLVIDELRQREASGRRMGVIAGAASRLDGLGGRVLIVD
                     DNERQAQRIAAELSIEHRPVVESDPEKARISAGGPVDLVIINGAAKGFDGLRFAASLR
                     SNERTRHLPVLAMVDPDERQRMVKALEIGVNDILPRPIDPQELAARVKTQIQRKRYTD
                     YLRNNLDHSLELAVTDQLTGLHNRRYMTGQLDSLVQRATLGGDPVAALLIDIDHFKKI
                     NDTFGHDVGDEVLREFALRLASNVRAIDLPCRYGGEEFTVIMPDTQLADAMRIAERIR
                     MHVSGSPFRVAQGKELLTVTISIGVSATLGEKDTPEALLKRADEGVYEAKASGRNAVV
                     GRVA"
     gene            1886..3586
                     /locus_tag="PLH31_04965"
     CDS             1886..3586
                     /locus_tag="PLH31_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQR88611.1"
                     /translation="MNTPLTFSAIVTALSLGLAVSSQAAPVAKPAPKLVVVISIDQFS
                     SSLFTQHRAEFTGGLARLSREGIVYPNGYQSHAMTETCPGHSTLLTGKHPNKTGIAAN
                     DWYEATLGKQVYCLGDDSVTLAHDAKGRAVSPKNMVATTYGDWLKAVSPTSRVFGVSG
                     KDRGAITLAGHKADGAFWMQPGFGFTTFVPPGETAEARLKPVAAFNARMMADLKKHPF
                     VWDYARSPLDALGRGSARRCKALEADYETGGRKWRAALPRPAPANEADYLRDLGTSPY
                     LDQVTLELAAELRDAYGLGDGPQVDVLAVSLSATDYIGHRYGTRGPEMCDQMVRLDER
                     LGAFLDRIGKTKGEVLVVLTADHGGADMPERLHDEGYDTGRVAARAWLAKLNATLRQD
                     LSLSHDPLLAEGGINAISIVGPDKKALPAVEHMRIAAPALALISRQPEVIEAFDVNTL
                     LTLAPERADVSPEEISVRERMRRSVYPGRSGDIVIAFRPDLVPASAGSSYVSGHGTPW
                     DYDRRVPILFWWKGGAPRERVLPLDTVDIAPTIAAVTGVKAPADVDGRCRPLGSGHDC
                     "
     assembly_gap    3628..3653
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3755..>3865)
                     /gene="rpmG"
                     /locus_tag="PLH31_04970"
     CDS             complement(3755..>3865)
                     /gene="rpmG"
                     /locus_tag="PLH31_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQR88612.1"
                     /translation="FYVTKKNARTKTEKMVLKKYDPVIRKHVEFKEGKIK"
BASE COUNT          653 a         1296 c         1258 g          632 t
ORIGIN      
        1 ggaagacaga agcggcgcga atccctcagg cgtcgccacg gaccgtatgg tcggggtcag
       61 gatgacgaag aaggtcctca tcgtggagga taatgagctg aacatgaagc tctttcatga
      121 tctgctcgaa gcccagggtt acgagaccct gcagacccgc gagggcctgc aagcgctatc
      181 gatcgcccgg gataatcgcc ccgacctgat tctcatggat atccagctgc cggagatctc
      241 gggactcgag gtcaccaagt ggctgaagga agacgaggac ctggcgcaca tccccgtggt
      301 ggccgtcacg gccttcgcga tgaagggcga cgaggagcgg atccgcgagg gcggctgcga
      361 ggcctatatc tccaagccga tctcggtggc ccacttcctc gacaccatca aacgcctgct
      421 ggagaagacc ccggcatgag cgcgcgtatc ctcgtcgtgg acgacattga agccaatgtc
      481 cgcctgctcg aggccaagct ttcggctgaa tattatcagg tctccaccgc cagcgacggc
      541 cccacggccc tggcgatggc ggccaatgat ctgcccgaca tcatcctgct ggacgtgatg
      601 atgcccggca tggacggttt caccgtctgc aagcgtctga aggacgatcc gatcacccgc
      661 catatcccgg tggtgctggt cacggcgctg gacggccgcg ccgaccgcat ccagggcctg
      721 gaagcgggcg cctcggactt tctgaccaag cccatcgacg acgtcatgct gtttgcccgc
      781 gtgcgcagcc tgacccgcct gaaactggtg atcgacgagc tgcgccagcg cgaggcctcg
      841 ggccgccgca tgggcgtgat cgctggagcc gcctcgcgcc tcgacggcct gggcggccgg
      901 gtgctgatcg tcgacgacaa tgaacgccag gcccagcgca tcgccgccga gctgagcatc
      961 gaacaccgcc ctgtggtcga gtccgacccg gaaaaggcgc ggatcagcgc cggcgggccg
     1021 gtcgatctgg tgatcatcaa cggcgcggcc aagggcttcg acggcctgcg tttcgccgcc
     1081 tccctgcggt cgaacgaacg cacccggcac ctgcccgtgc tggccatggt cgatccagac
     1141 gagcgccagc ggatggtcaa ggcgctggaa atcggcgtca acgacatcct gccgcgcccg
     1201 atcgatccgc aggaactggc ggcgcgggtg aaaacccaga tccagcgcaa gcgctacacc
     1261 gactatctgc gcaacaatct cgaccacagc ctggaactgg cggtcactga ccagctcacg
     1321 gggcttcaca accgtcgcta catgaccggc cagctcgatt cgctggtcca gcgcgcgacg
     1381 ctgggcggcg atccggtcgc ggcgctgctg atcgacatcg accacttcaa gaagatcaac
     1441 gacaccttcg gccacgatgt gggcgacgag gtgctgcgcg agttcgccct gcgcctggcc
     1501 tccaatgtcc gcgccatcga cctgccttgc cgctatggcg gtgaggagtt cacggtgatc
     1561 atgcccgaca cccagctggc tgacgccatg cgcatcgccg agcggatccg catgcacgtg
     1621 tccggctcgc ccttccgggt cgcccagggc aaggaactgc tgaccgtcac gatctcgatc
     1681 ggcgtctcgg cgacgctggg tgaaaaggat actcccgagg ccctgctgaa gcgcgccgac
     1741 gagggcgtgt acgaggccaa ggcttctggc cgcaatgcgg tggtgggccg cgtcgcctga
     1801 cgatatcgcg gtctcgcggg cagttatccg tcgtcggaca atgttcgaaa cttgcgtgaa
     1861 cgccgcgttc gggcaatttg cccgaatgaa cactccgctg accttttccg ccattgtgac
     1921 ggccctttcg ctgggcctcg ccgtttcgtc ccaggccgcg ccggtcgcca agcccgcgcc
     1981 aaagctagtg gtggtcatct cgatcgacca gttcagctcc agcctgttca cccagcaccg
     2041 cgccgagttc accggcggcc tggccaggct gtcgcgcgag gggatcgtct atcccaacgg
     2101 ctatcagtcg cacgccatga ccgagacctg tccggggcac tccaccctgc tgacgggcaa
     2161 gcatcccaac aagacgggca tcgccgccaa tgactggtac gaagccaccc tgggcaagca
     2221 ggtctattgc ctgggcgacg acagcgtcac cctggcccat gacgccaagg gccgggcggt
     2281 cagcccaaag aacatggtcg ccaccaccta tggcgactgg ctgaaagccg tctcgccgac
     2341 cagccgggtg ttcggcgtct cgggtaagga tcgcggcgcg atcaccctgg ccggccacaa
     2401 ggccgatggc gcgttctgga tgcagccggg ctttggcttc accaccttcg tgccgcccgg
     2461 cgagacggcc gaggcgcggc tcaagcccgt ggcggccttc aacgcccgga tgatggccga
     2521 cctgaagaag catccgttcg tctgggacta tgccaggagc ccgctggacg ccctggggcg
     2581 cggctcggcc aggcgctgca aggcgctgga agccgactac gagaccggcg gccgcaagtg
     2641 gcgcgcggcc ctgccgcgac cggcgccggc gaacgaggcc gactatctgc gcgacctggg
     2701 aactagcccc tatctggatc aggtgaccct cgaactggcc gccgagctgc gcgacgccta
     2761 cggcctgggc gatggcccgc aggtggatgt gctggccgtc agcctgtcgg ccaccgacta
     2821 tatcggacac cgctacggca cgcggggccc cgagatgtgc gaccagatgg tgcgcctgga
     2881 cgagcggctg ggcgcgtttc tcgacaggat cggcaagacc aagggcgagg ttctggtggt
     2941 gctgacggcc gaccatggcg gcgccgacat gcccgagcgg ctgcacgacg aaggctatga
     3001 caccggccgc gtggccgccc gcgcctggct ggccaagctg aacgcgacgc tgcggcagga
     3061 cctttcgctg tcccatgacc ccctgctggc cgagggcggc atcaacgcca tcagcattgt
     3121 gggccccgac aagaaggccc tgcctgcggt cgagcacatg cgtatcgccg cgcccgccct
     3181 ggccctgatc tcgcgtcagc ccgaggtgat cgaggccttc gacgtcaaca ccctgctgac
     3241 cctggccccc gagcgcgccg atgtctcgcc cgaggaaatc tcagtgcgcg agcgcatgcg
     3301 ccgcagcgtc tatccgggcc gttcgggcga catcgtcatc gccttcaggc ccgatctggt
     3361 gccggcttcg gcggggtcga gctatgtgtc cggccatggc accccctggg actatgaccg
     3421 ccgggtgccg atcctgttct ggtggaaggg cggcgcgccc cgcgaacgtg tcctgccgct
     3481 cgacacggtg gacatagccc cgaccatcgc cgcggtgacg ggcgtgaagg cgccggcgga
     3541 tgtggacggg cggtgccggc cgctgggcag cgggcatgac tgctagggtc tagctcctcc
     3601 ccctctgggg gaggtggccc ggagggcnnn nnnnnnnnnn nnnnnnnnnn nnngtcggct
     3661 ttgccgacag ctcccccaga ggggggagca tcaatcaaaa acgcccggct atcgctagcc
     3721 gggcgttttg tcgtgtcgtg ccggtcaggc agatctactt gatcttgcct tccttgaatt
     3781 cgacgtgctt gcggatgacc ggatcgtact tcttcagcac catcttttcg gtcttggtgc
     3841 gggcgttctt cttggtcacg tagaa
//
LOCUS       DAOMEO010000164         3857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2757_length_3857_cov_2.053393, whole genome shotgun sequence.
ACCESSION   DAOMEO010000164 DAOMEO010000000
VERSION     DAOMEO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_3857_cov_2.053393"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLH31_04975"
     CDS             <1..213
                     /locus_tag="PLH31_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQR88613.1"
                     /translation="DNALRFDVSSQGGQNGQNAFFHFNDGQDGRRAWSGKAFQSAQND
                     DAPILSASDLLPGLRMAPDSGLDIRI"
     gene            228..>842
                     /locus_tag="PLH31_04980"
     CDS             228..>842
                     /locus_tag="PLH31_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HQR88614.1"
                     /translation="MATAVTGTGSVLDKINNSRNSLASSEETFLKLLTTQMKNQDPLS
                     PMDSNAFTAQIVQMTGVEQQLVTNDLLAALVGMNDGGMTDAVNMMDKLATVETDKSVL
                     KNNEASWAYNQSRAATSVKIEVVDQYGKTIRTALPEDMSKGDHTFEWDGKSDAGVQQP
                     AGGAYTLKITALDSEGSKLSVTSKGRVEGIVTAITSENGQHMVTI"
     assembly_gap    843..852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1080..>1387
                     /locus_tag="PLH31_04985"
     CDS             1080..>1387
                     /locus_tag="PLH31_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HQR88615.1"
                     /translation="MSINSAMLSGVSGLIANSSALAAISDNISNVNTVGYKRSTANFS
                     TLVTSQSKNATYSAGGVKALTHQFITQQGLTQSSTSNLDLSISGAGFFVGTEKPEGLT
                     A"
     assembly_gap    1388..1397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1398..2751
                     /locus_tag="PLH31_04990"
     CDS             <1398..2751
                     /locus_tag="PLH31_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HQR88616.1"
                     /translation="TRAGSFQLDNLGYLKNDAGLYLQGWLADPVTGDITPDPSDLTQL
                     DSINVGSVGGTAEKTTRAAVNANLRSEQPLSAQVKFSVSKASVPETVPASTGAYTVSY
                     AETATAGIYDVEIRKGTTLVGTGTGDYSSGTLATPTGILATDPIDLSGAGTGPYVAFA
                     GLGVGTDADLSTGNLYNSATNSMSDYALDATTGVKPDFEIQIPVSDSKGGQRTVTLSL
                     LKSNTPNEWYAELRAKPGDLDNVANGLISSGRVTFTTDGKLQSVGNLFGGTNPTSITI
                     GASDPNNVAATPPRWADSLGIDSQTLQIDLANAAGGLTQFNSQSVVQSVNTNGTAFGN
                     LTNIEVDDEGYVSAIFDNGVTRRIAQVAVATFSNANGLKGVNGNAYRVTNESGTYSLK
                     TPGQGGAGVIAPSTLEASTVDLSTEFTGLITTQRAYSASSKIITTADQMLEELLSIKR
                     "
     gene            2921..3202
                     /locus_tag="PLH31_04995"
     CDS             2921..3202
                     /locus_tag="PLH31_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HQR88617.1"
                     /translation="MLQQQRTNSRGEKYVIGPTGAPLTLSDLPPPETQRWVIRRKAEV
                     VAAVRGGLLSLDEACDRYKLTNEEFLNWQQSIDKHGLAGLRTTRLQQYR"
     gene            3449..>3857
                     /locus_tag="PLH31_05000"
     CDS             3449..>3857
                     /locus_tag="PLH31_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HQR88618.1"
                     /translation="MDSFLSAIQRFGVGRLAALIGVGAGVVAILVALIMFMGKEPTEL
                     LYSNLDLKEASQVTQALDQAGVKYETKGDGSTIMVARDKVASARLLVAGKGLVTSGSI
                     GYEIFDSNNALGQTDFVQQLNRQRALQGELERTI"
BASE COUNT          788 a         1284 c         1117 g          648 t
ORIGIN      
        1 gataacgccc tgcgcttcga tgtgtcgagc cagggcggcc agaatggcca gaacgccttc
       61 ttccatttca acgacggcca ggatggccgc cgggcctggt cgggcaaggc cttccagtcc
      121 gcccagaacg acgacgctcc catcctttcc gcttcagatc tgctgcccgg gctgcgaatg
      181 gcccccgaca gcggtctgga catcagaatt tagagagagg ccgcgccatg gctaccgccg
      241 ttaccggcac gggttccgtg ctcgacaaga tcaacaactc ccgcaactcc ctggccagca
      301 gcgaggaaac cttcctcaag ctgctgacca cccagatgaa gaaccaggat ccgttgtcgc
      361 cgatggattc caacgctttc acggcccaga tcgtccagat gaccggcgtc gagcagcagc
      421 tcgtcaccaa tgacctgctg gccgccctgg tcggcatgaa cgatggcggc atgaccgacg
      481 ccgtcaacat gatggacaag ctggcgactg tcgaaaccga caagtcggtg ctgaagaaca
      541 acgaagccag ctgggcctat aaccagagcc gcgcggccac gagcgtgaag atcgaggtcg
      601 tggaccagta cggcaagacc atccggaccg cactgcccga ggatatgtcc aagggcgacc
      661 acacctttga atgggatggc aagagcgacg cgggcgtcca gcagcccgcc ggcggcgcct
      721 ataccctgaa gatcacggcg ctggacagcg agggcagcaa gctttcggtg acctcgaagg
      781 gccgcgtcga ggggatcgtg accgcgatca ccagcgagaa cggccaacac atggtgacca
      841 tcnnnnnnnn nnccgtgatc ggtgtgacca acccgccggc cactccagct ccggctccga
      901 ccgccaacac caatacaacc ccgacgaaca cagccgcagc ggcctgatcg tcacgacctg
      961 atgaaccggg cgtcagaatg acgccgacgc ggctcttccc ggaaatggtt tccgcagagc
     1021 ccaaacgcaa accgcgctga acctccccgt cattcgacat tcaacgcagg aatttcgtca
     1081 tgagcatcaa cagcgccatg ctttcgggcg tctcgggcct gatcgccaac tcctcggccc
     1141 tggccgcgat ctcggacaac atctcgaacg tcaacacggt gggctacaag cgctcgaccg
     1201 ccaatttctc gacgctggtc acctcgcaga gcaagaacgc gacctacagc gccggcggcg
     1261 tgaaggccct gacccaccag ttcattacgc aacagggcct gacccagtcc tcgacctcga
     1321 acctcgacct gtcgatctca ggcgcgggct tcttcgtcgg caccgagaag cctgaaggcc
     1381 tgaccgcnnn nnnnnnncac gcgcgccgga tcgttccagc tggacaatct gggctatctg
     1441 aagaacgacg ccggccttta tctgcaaggc tggctggccg atccggtgac tggcgacata
     1501 acgcccgacc cctcggacct gacccagctg gactcgatca atgtcggttc ggtcggcggc
     1561 accgccgaaa agaccacccg cgcggccgtc aacgccaacc tgcgctccga gcagccgctt
     1621 tcggctcagg tcaagttctc cgtcagcaag gccagcgtgc cggaaaccgt tccggccagc
     1681 accggcgcgt acacggtcag ctacgccgag accgcgacgg cggggatcta tgacgttgaa
     1741 atccgtaagg gcaccacgct ggtgggcacg ggcacgggcg actatagctc cggcaccctg
     1801 gccaccccca cgggcattct ggccaccgac ccgatcgatc tcagcggcgc cggcaccggc
     1861 ccctatgtgg ctttcgcggg ccttggcgtc ggcactgacg ccgacctgtc gaccggcaat
     1921 ctctacaatt cggcgaccaa ctcgatgtcg gactacgcgc tcgacgcgac caccggcgtc
     1981 aagccggact tcgagatcca gatccccgtg tcggactcca agggcggcca gcgcacggtc
     2041 accctgtcgc tgctgaagag caacacgccc aacgagtggt atgcggaact gcgcgccaag
     2101 ccgggcgatc tggataacgt cgccaacggc ctgatcagct ccggccgcgt gaccttcacg
     2161 acggacggca agctgcagag cgtcggcaac ctgttcggcg gcaccaaccc gacctcgatc
     2221 accatcggcg cctccgaccc caacaacgtg gcggctacgc cgccccgctg ggctgacagc
     2281 ctgggtatcg acagccagac cctgcagatc gacctggcca acgccgccgg cggcctgacc
     2341 cagttcaaca gccagtcggt cgtccagtcg gtcaatacca acggcaccgc cttcggcaac
     2401 ctgaccaata tcgaagtcga tgacgaaggc tatgtttcgg ccatcttcga caacggcgtc
     2461 acgcgccgga tcgcccaggt ggccgtggcg accttctcaa acgccaacgg tctgaagggc
     2521 gtcaacggca acgcctaccg cgtcaccaat gaaagcggca cctatagcct caagactccg
     2581 gggcagggcg gagcgggtgt cattgcgcct tcgaccctag aagcctcgac tgtcgatctg
     2641 tccactgagt tcaccggact gatcacgaca caacgcgcct actccgcttc atccaagatc
     2701 atcactaccg ctgaccagat gcttgaggaa ctcttgagca ttaagcgata gttataggta
     2761 ttaactcgat ttaatagccg tgttccacaa cggcacacta aagacttcag ggagaagcta
     2821 ggtgtttagg cctttcttta ctattggaat taagccgctg ttattggctg gcgcctatag
     2881 tgaccttcaa cagtgatggt tgtgggaaag gcgtaaagcc atgttgcagc agcagcgcac
     2941 gaacagccgg ggtgagaagt acgttatcgg tccgaccggt gcgcctctaa ctctctccga
     3001 cttgccgcct ccggaaaccc agcgttgggt gatccggcgt aaagccgaag tggtcgccgc
     3061 cgttcgcggt ggtctgcttt cgctcgacga ggcttgcgat cggtacaagc tgaccaatga
     3121 ggaattcctc aattggcagc agtcgatcga caagcacggt ctggccggtc ttcggaccac
     3181 cagactccag cagtaccgtt agacgcggga ctgaaagata tgggctttcc gaccgctcgc
     3241 ggccggaaac gcctcctgga acgttcagcg gccgcgccct tcccggggcg cggccgcgac
     3301 gttttggcgc ttccggcagc gctctggaga tccaaatata aacgcctcgt ttaccttgaa
     3361 ctgggtaaat cctgcctagc gacggacgcc tcccggcaac cgacgccatt gtcccccgtc
     3421 ggggtggctt tctgggggat tggcctgagt ggatagcttt ctgagcgcga tacagaggtt
     3481 cggcgtcggc cggctcgccg ccctgatcgg cgtcggcgct ggcgttgtcg cgatccttgt
     3541 cgctctgatc atgttcatgg gtaaggagcc gactgagctc ctgtattcca acctcgacct
     3601 caaggaggcc tcgcaggtca cccaggccct ggatcaggcc ggcgtgaagt acgagaccaa
     3661 gggtgacggc tcgaccatca tggtggcccg cgacaaggtc gcctcggccc gcctgctggt
     3721 cgccggcaag gggctcgtga cgtccggctc gatcggctac gaaatcttcg acagcaacaa
     3781 cgccctgggc cagaccgact tcgtccagca acttaaccgc cagcgcgccc tgcagggcga
     3841 gctggagcgg acaatca
//
LOCUS       DAOMEO010000165         3846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2764_length_3846_cov_2.110525, whole genome shotgun sequence.
ACCESSION   DAOMEO010000165 DAOMEO010000000
VERSION     DAOMEO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_3846_cov_2.110525"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLH31_05005"
     CDS             complement(<1..794)
                     /locus_tag="PLH31_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQR88619.1"
                     /translation="MIRPTLIALALALSVTAAHAAPKPGQAQFRNFTYEGADSVDAQV
                     KLAPGQYRNPILKGFYPDPSITRVGDDFYLVNSTFAYFPGLPVWHSTDLVNWTQIGNA
                     IDRPGMLDFKRLGLSRAVFAPAIEEHEGTFYILNTCVDCQGNFLITAKDPKGPWSDPV
                     WLPTVGGIDASIFFDDDGRAWILNNDEPPGPAEYPGHRAIWIQEYDAKTQKIIGPRTL
                     LLDKGVDPSTKPIWPEGPHILKKDGWYYLTAAEGGTAEGHSQVVLRA"
     gene            complement(937..2088)
                     /locus_tag="PLH31_05010"
     CDS             complement(937..2088)
                     /locus_tag="PLH31_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQR88620.1"
                     /translation="MRCFPTALSRRQALGLGAAAGAMTALPALAAGTPSLASLARDKG
                     LHFGSAVGAGPVGSLTSSFEDARLRQILKDECSVLVPENELKWYVIRAAGPQAFDFSR
                     ADRIAAFAKANGQALRGHTLVWHHPQWFPAWIKTHDFGPQPKAAAEAMLVEHINKTCE
                     RYPQMISWDVINETVDPADSAIRRTVFSDILGQHETLDLCFHTARAAAPKARLVYNDY
                     MSWEVGNEKHRYGVLKLLEGFRKRGTPVDALGVQAHIGAENADSFTGFGKPQEKEWKA
                     FIDEATGMGYDLMITEFDVHDKGLVYDFAPRDAAVAAYAKGYLDMMLSYRQTKELLAW
                     GMVDKYSWLQNQWLREDKKPKRPTLYDDNYQPKPLREAVAAALKAAPVR"
     gene            complement(2228..>3846)
                     /locus_tag="PLH31_05015"
     CDS             complement(2228..>3846)
                     /locus_tag="PLH31_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88621.1"
                     /translation="WAERDQTTRFSTYNWGVLSEIWGEGGPVWMSDRIDGVPNAGGLP
                     GTTGTDSTAIAMLNGFPNFMRGQTPLPTGTDSRLFYGDNTAVNYKAASDFALSVGDEW
                     RARNGANGCAQNWVPLAMRCNVVAGSPFLPQEINPVNEVNKAAYVVARFKGDMGAARV
                     SGNIGLRYTTTTREADGFQAFPNINFATDAECAIVPPGEQPTPFCALTPAVRASARLW
                     ANGAVNATTAKGDFNYWLPSFNLKIAMPGGLQYRLGASKTVTPPDIGLTRNYYNLTLN
                     TNADGISNGQPSGNVTVGNPYLKPTKSTNLDASVEWYFDAVGSLTFAAFYKELTDVAT
                     NTTARLPFTNNGATFDVLVTTPGNSDQKAKVKGFEIGYQQFYDFLPKPLDGFGINANY
                     SYIDSKGVPQSTLSATDPDVAAGRVTTVNTSLLPLQGLSKHNANFAAIYEKGAISARL
                     AYNWRSDFLLTVRDVIVPFAPIMNEATGQLDGSLFYTVNPKMKIGVQGVNLLNETTMT
                     SQVLNDELLKTGRSWFMNDRRYTFVVRASF"
BASE COUNT          634 a         1175 c         1339 g          698 t
ORIGIN      
        1 gcccgtagca ccacctggct gtggccctcg gccgtgccgc cctctgcggc ggtcagatag
       61 taccagccgt ccttcttcag gatatgcggg ccctcgggcc agatcggctt ggtcgacgga
      121 tcgacgccct tgtccagcag cagggtgcgc gggccgatga tcttctgggt tttggcgtca
      181 tattcctgga tccagatggc ccggtgaccc ggatactccg caggcccggg cggctcgtca
      241 ttgttgagga tccaggcgcg gccgtcatcg tcaaagaaga tcgaggcgtc gatgccgccc
      301 acggtcggca gccacaccgg gtctgaccag ggccccttgg ggtccttggc ggtgatcagg
      361 aaattgccct ggcaatcgac gcaggtgttg aggatataga acgtgccctc atgctcctcg
      421 atggccggcg cgaacacggc gcgcgacagg cccaggcgct tgaagtccag catgccgggg
      481 cggtcgatgg cgttgccgat ctgggtccag ttgaccagat cggtggagtg ccagaccggc
      541 aggccgggga aataggcgaa ggtcgaattg accagataga agtcgtcgcc cacccgcgtg
      601 atgctggggt cgggatagaa gcccttcagg atcgggttgc ggtactggcc cggggccagc
      661 ttgacctgcg cgtcgacgga atcggcgcct tcataggtga agttgcggaa ctgggcctgg
      721 cccggtttgg gcgcggcgtg agcggcggtc accgacaggg ccagggcgag cgcgatcagg
      781 gtcgggcgga tcattgattt actccagaca tttcggacag ccacttaccc cctccgctgc
      841 gcgcccctcc cccgggaagg ggaagatgag agcgccttcc ccctccgggg gaggacgagc
      901 gcgtagcggt caggaggggg cctgcggcat ttgcagttat cgcaccggcg ccgccttcaa
      961 cgccgcggcc accgcctcgc gcaacggctt gggctggtag ttgtcatcat agagcgtcgg
     1021 gcgcttgggc ttcttgtcct cgcgcagcca ctggttctgc agccacgagt atttgtcgac
     1081 catgccccag gccagaagct ccttggtctg acgataggac agcatcatgt cgagatagcc
     1141 cttggcatag gccgccaccg ccgcgtcgcg gggcgcgaaa tcatagacca ggcctttgtc
     1201 gtggacgtcg aactcggtga tcatcagatc atagcccatg ccggtggcct catcgatgaa
     1261 ggccttccac tccttctcct gcggcttgcc gaaaccggtg aagctgtcgg cgttctcggc
     1321 gccgatatgg gcctgcacgc ccagggcgtc gacgggcgtg ccgcgttttc tgaagccttc
     1381 cagcaacttg agcacgccgt agcggtgctt ctcgttgccg acctcccagc tcatatagtc
     1441 gttatagact aggcgagcct tgggcgctgc ggcgcgggcg gtgtggaagc acaggtccag
     1501 ggtctcgtgc tggcccagga tgtcggagaa gacggtgcga cggatcgcgc tgtcagccgg
     1561 atcgaccgtc tcgttgatca cgtcccacga gatcatctgc gggtagcgct cgcaggtctt
     1621 gttgatgtgc tcgaccagca tggcctcggc ggccgccttg ggctgggggc caaaatcgtg
     1681 ggtcttgatc caggcgggga accactgcgg atggtgccag accagggtgt ggccgcgcag
     1741 ggcctggccg ttggccttgg cgaaggcggc gatgcggtca gcgcgcgaaa agtcgaaggc
     1801 ctgagggccg gcggcgcgga tcacgtacca cttcagctcg ttctccggca ccagaacgct
     1861 gcactcgtcc ttgaggatct ggcgcaggcg ggcgtcctcg aacgagctgg tcagggaacc
     1921 gacggggccc gcgccgacgg cgctgccaaa gtgcaggccc ttgtcacggg ccaggctggc
     1981 cagggacgga gtcccggcgg ccagggccgg aagggccgtc atggcgccgg cggcggcgcc
     2041 cagacccaga gcctgacggc gggacaaagc cgtgggaaag caacgcatcg ctcaagcctc
     2101 caaaaaaaac gcgcaccgcc ggagacgatg cgcggaaaga agaactgcaa gtgtcgagcc
     2161 ctccggggag aacccatccg aaagtcgcgc cggcccgggg gagaccaaac cggcgcggct
     2221 ttcgaaccta gaagctggcg cgcaccacga aggtgtagcg ccggtcgttc atgaaccagg
     2281 aacggccagt cttgagcagt tcgtcgttca ggacctggct ggtcatggtg gtttcgttga
     2341 gcaggttcac gccctgcacg ccgatcttca tcttcggatt gaccgtgtag aacagcgagc
     2401 cgtccagctg gccggtggcc tcgttcatga tcggtgcgaa gggcacgatc acgtcgcgga
     2461 ccgtcagcag gaagtccgag cgccaattgt aggccagacg ggccgagatg gcgcccttct
     2521 catagatggc cgcgaagttg gcgttgtgct tggacaggcc ctgcagcggc agcaggctgg
     2581 tgttgaccgt cgtaacgcgg ccggcggcca cgtcgggatc ggtcgccgac agcgtgcttt
     2641 gcggcacgcc cttgctgtcg atgtagctgt agttggcgtt gatgccaaaa ccgtccagcg
     2701 gcttgggcag gaagtcgtag aactgctggt agccgatctc gaagcccttg accttggcct
     2761 tctggtcgga attgcccggc gtggtgacca gcacatcgaa cgtcgcgccg ttattggtga
     2821 acggcaggcg cgcggtcgtg ttggtggcga cgtcggtcag ctctttgtag aaggccgcga
     2881 aggtcagcga gcccacggcg tcgaagtacc actcgaccga ggcgtccagg ttggtcgact
     2941 tggtcggctt cagatagggg ttgcccaccg tgacgttgcc gctgggctgg ccgttgctga
     3001 tgccgtcggc gttggtgttc agcgtcaggt tatagtagtt gcgcgtcagg ccgatatcgg
     3061 gcggcgtgac cgtcttggac gcgccaagac gatattgcag cccgcccggc atggcgatct
     3121 tcaggttgaa gctgggcagc cagtagttga agtcaccctt ggccgtggtc gcattgaccg
     3181 cgccattggc ccacagccgc gccgaggccc gcaccgccgg agtcagcgcg cagaacgggg
     3241 tcggctgttc gcccgggggc acgatggcgc actctgcgtc ggtggcgaag ttgatgttcg
     3301 ggaacgcctg gaagccgtcg gcctcgcggg tggtggtggt gtaacgcagg ccgatattac
     3361 cgctcacccg cgccgcgccc atgtcgccct tgaagcgagc gacgacatag gccgccttgt
     3421 tgacctcgtt aaccgggttg atttcctgcg gcaggaacgg cgagccggcg acgacattgc
     3481 atcgcatggc cagcggcacc cagttctgag cgcagccgtt tgcgccgttg cgggcgcgcc
     3541 attcgtcgcc gaccgacagg gcgaagtccg aagcggcctt gtagtttacc gccgtgttgt
     3601 cgccgtagaa cagacgcgag tccgtgcccg tgggcagcgg ggtctggccg cgcatgaagt
     3661 tggggaagcc gttcagcatc gcgatggcgg tgctgtcggt ccccgtcgtg ccgggcaggc
     3721 cgccggcgtt tggcacgccg tcgatccggt cgctcatcca caccgggccg ccttcgcccc
     3781 agatttcgct gagcacaccc cagttatagg tcgagaagcg ggtggtctgg tcgcgttcgg
     3841 cccagc
//
LOCUS       DAOMEO010000166         3843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2766_length_3843_cov_2.746304, whole genome shotgun sequence.
ACCESSION   DAOMEO010000166 DAOMEO010000000
VERSION     DAOMEO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_3843_cov_2.746304"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLH31_05020"
     CDS             complement(<1..843)
                     /locus_tag="PLH31_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQR88622.1"
                     /translation="MSPYNLAALLSPTSLAVIGGSDAPGSVGQVVVENLVSGGFTGPI
                     YLVNPRPLSIAGTRWKATIAELPEAPELAVVAVPAAAVPQVIADLGAAGVKIAVILSA
                     GLTQANGLREAMLQAARPSGLRIIGPNGLGFLAPHAGLNASFARGAPQPGGLALISQS
                     GALVSGLIDWATTRQLGFSGVVSVGDMAEVDLGDLIDLFAADPHTDAILLYIEGVTNP
                     AKFMSAARAAARVKPVIALKAGRSAASGKAVRSHTGALAGAYDVHACAFERAGIVQVE
                     TLTDL"
     gene            966..>1571
                     /locus_tag="PLH31_05025"
     CDS             966..>1571
                     /locus_tag="PLH31_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88623.1"
                     /translation="MEAAMCTGMIGSRRLSWADCPGDREGEDHMESGSSLQGGQIRSY
                     GAALIAFGVAVGLRWLLAPWLGDDGAYLLFTVPIIAAVLFGGVGASLVAAALGLIAGF
                     VFSTRSGADGLASIEVLIFLLVATSLVLVGAQVRRLNPLSTAGGPTAAARSSLAHDLA
                     SELNLLIDGATEYAICMLDLEGRVLIWNPGGQRLLGWSESEI"
     assembly_gap    1572..1581
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1582..2923
                     /locus_tag="PLH31_05030"
     CDS             <1582..2923
                     /locus_tag="PLH31_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR88624.1"
                     /translation="YPQDARDAGKPQEDLNRTRAEGKVEQEAWLLRKDGSEFLAHISI
                     TALHDQSGALRGFGKVLRNVTDQRASERALDAGAEHLRSILSTVPDAMVVIDDRGKIL
                     SFSTAAERLFGFQEADVLGENVSCLMPSPDRERHDDYILRYLQTGERKVIGMGRVVVG
                     MRKDGTTFPMELSVGEATGHGPRVFTGFVRDLTERQRTQARLEELQAELIHIARVSGM
                     GTMASTLAHELNQPITAVANYVEAVRDLLANPDPDDLPMIREALGDAAGEAMRAGHIV
                     RRLRDFVARGEVEKSVEDLPSVIHEAAALGLMGARDQGLEPVFDLDPVASPVLIDRVQ
                     IQQVVINLARNAVEAMQGMEEPRLWLAANDQGDGLVRVTVSDNGPGVAPSIAGQLFTA
                     FISTKAEGMGLGLSICRTIIEANGGRIWHEPRSGGGSQFHFTLVKVEGESIHEQ"
     gene            2913..3533
                     /locus_tag="PLH31_05035"
     CDS             2913..3533
                     /locus_tag="PLH31_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88625.1"
                     /translation="MSSRRMIHIVDDEEAIRRSAGFMLKTSGYAVATYASGDAFLQAA
                     KDLEIGCVLLDVRMPGMDGLEVQKVMAERGIAMPVVVLTGHGDITVAVRAMKGGAVDF
                     IEKPFEKAVLLSAIVAAFERLDDVEARSARASDAAIAIAALTSREQDVLRGMVQGLPN
                     KTIAFDLGISPRTVEVHRANLMAKLQVKSLSEALRIAFAASLQDEI"
     gene            3618..>3843
                     /locus_tag="PLH31_05040"
     CDS             3618..>3843
                     /locus_tag="PLH31_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88626.1"
                     /translation="MTQDTPLKHEGRARLLLVEDDPGVRRSLQLLLHGRGFEVRAYAS
                     GLALLADPTALDAVCLVSDYRMPEIDGLLIL"
BASE COUNT          645 a         1180 c         1330 g          678 t
ORIGIN      
        1 gagatcggtg agggtctcga cctggacgat gccggcccgt tcaaaggcgc aggcatggac
       61 gtcgtaggcg ccggccaggg cgccggtgtg agagcggaca gccttgccgc tggcggcgct
      121 gcgtccggcc ttcaaggcga tgaccggctt gacgcgcgca gcggcgcggg ccgccgacat
      181 gaacttggcc gggttggtga cgccctcgat atagagcagg atggcgtcgg tgtggggatc
      241 ggccgcgaag agatcgatca ggtcgccaag gtcgacctcg gccatatcgc ccaccgagac
      301 gacccccgaa aagcccagtt ggcgcgtcgt cgcccaatcg atcaggccgc tcaccagggc
      361 gccgctttgc gagatcagag ccaggccgcc gggttgtggc gcgccgcggg cgaaggaggc
      421 gttgaggccc gcgtggggcg ccaggaagcc caggccgttg ggaccgatga tgcgcaatcc
      481 agagggcctg gccgcctgga gcatggcttc gcgcaggcca ttggcctggg tcaggcccgc
      541 tgagaggatc acggcgatct tgaccccggc cgcgccgaga tcggcgatca cctgggggac
      601 ggcggcggct ggaacagcca ccacggccag ttcaggggcc tcgggcaatt cggcgatcgt
      661 ggccttccag cgtgttcccg caatggatag aggtcgcgga ttgaccaggt agatgggacc
      721 ggtgaagccg ccagacacca gattttccac cacgacctga cccacagagc ctggggcgtc
      781 ggatccgccg atcacggcca gcgaggtcgg ggagagcagg gcggcaagat tgtacggcga
      841 catggagagc ctcaagcaac agggcccgtc ggggcgggcc gtcggctgca aaggacccgc
      901 caagacgctt gtatgccatc cgtagaatcc cgcatgtgat ttggcgcacg catggtccag
      961 ccgggttgga ggccgcgatg tgcacgggca tgatcggatc gcggcgtttg tcttgggcgg
     1021 attgtcccgg cgacagagaa ggtgaagacc acatggagtc cggttcgtcc ctccagggag
     1081 gccagatcag gagctacggc gcggctctta tcgcctttgg ggtcgccgtc ggcctacggt
     1141 ggctgctggc tccctggctg ggcgacgacg gcgcctatct gctcttcacc gttcccatca
     1201 tcgctgccgt cctgttcggc ggcgtcggcg ccagcctggt cgcagcggcc ctgggcctga
     1261 tcgccggctt cgttttcagc acccgtagcg gtgcagatgg cttggcgtcg atagaggtgc
     1321 tcatcttcct gttggtggcg acgagccttg tcctggtggg cgctcaggtc agacgtctga
     1381 atccgctttc gacggctggc gggccgaccg ccgcagccag atccagcttg gcccacgacc
     1441 tggccagcga gctcaacctc ctcattgacg gcgctacgga gtacgccatc tgcatgctcg
     1501 acctggaggg gcgggtgctg atctggaatc ccggcggaca gagacttctg ggctggagcg
     1561 agtccgagat cnnnnnnnnn nctatcccca agacgcccgg gacgctggca aaccgcaaga
     1621 ggatctgaac cggacccgcg ctgaggggaa ggtcgagcaa gaggcctggc ttctgcggaa
     1681 ggatggctcg gagtttctgg cccacatctc catcaccgca ttgcacgatc aatctggcgc
     1741 cttgcgaggc tttgggaagg tgttgcgcaa cgtcaccgac cagcgcgcga gcgagcgggc
     1801 cctggatgcc ggcgccgagc atctgcgatc cattctgtcc accgtccctg acgccatggt
     1861 ggtcatcgat gatcggggca agatcctgtc tttcagcacc gccgccgaac gcctcttcgg
     1921 gttccaggag gccgacgtgc tgggtgagaa tgtttcctgc ctgatgccct caccggatcg
     1981 cgagcgccat gacgactata tcctgcgcta tctccagacc ggtgagcgca aggtcatcgg
     2041 catggggcgt gtcgtcgtcg gcatgcgcaa ggatggcacg acctttccga tggagctgtc
     2101 ggtcggtgaa gcgaccggcc acggtccgcg cgtcttcacc ggctttgtcc gcgaccttac
     2161 cgagcgccaa cgcacccagg cgcggcttga ggagttgcag gccgagttga tccatatcgc
     2221 ccgggtcagc gggatgggga ccatggcttc gaccctcgcc catgagctca accagccgat
     2281 caccgccgtg gccaactatg tggaggcggt ccgtgatctg ctggccaatc ccgatcccga
     2341 cgacctgccg atgatccgcg aggcgctggg cgacgcggcc ggggaggcca tgcgcgcggg
     2401 tcatatcgtc aggcggctgc gcgatttcgt agcccggggg gaggtcgaga agagtgttga
     2461 ggacctgccc tccgtcatcc atgaagccgc tgcgctgggc ttgatggggg cccgcgacca
     2521 ggggcttgag ccggtgtttg atctcgaccc ggtcgcgtcg cctgtcctga tcgaccgggt
     2581 gcagatccag caggtcgtca tcaatctggc tcgtaacgcg gtagaggcca tgcaaggcat
     2641 ggaagagcca aggctgtggc tggccgcaaa tgatcagggc gatgggctgg tgcgcgtgac
     2701 cgtctctgat aacggccccg gcgtggcgcc cagcatcgcg gggcagctgt tcacggcctt
     2761 catcagcacc aaagccgagg gtatggggct ggggctgtcg atctgccgga caatcataga
     2821 ggccaatggt ggccgcatct ggcatgagcc gcgctctggc ggcggctcac agtttcattt
     2881 tacgctcgtc aaggtcgagg gggagtccat ccatgagcag taggcggatg atccatatcg
     2941 tcgatgacga agaggcgatc cgtcgctcgg ccggcttcat gctcaagacc tcgggctatg
     3001 ccgtcgcgac ctacgcctcc ggggacgcct tcttgcaggc ggccaaggat ctcgagatcg
     3061 gttgcgtgct gctggatgtg cgtatgccgg ggatggatgg cctggaggtc cagaaggtca
     3121 tggccgaacg cggcatagcc atgccggtgg tggtgctaac cggtcatggg gacatcaccg
     3181 ttgcggttcg ggccatgaag ggcggggcgg ttgatttcat cgaaaagccg ttcgagaagg
     3241 ctgtgctgct gagcgccatt gtggccgcgt tcgagcggct tgatgacgtc gaggcgcggt
     3301 cggcgcgcgc aagcgacgcg gccatcgcca tcgcagccct caccagccgg gagcaggacg
     3361 tattgcgggg catggtccag gggctcccga acaagaccat cgcttttgat ctgggcatct
     3421 cgccgcgcac cgtggaagtg caccgcgcca atctgatggc caagctgcag gtcaagagcc
     3481 tgtcagaggc tctgcggatc gccttcgccg ccagcctgca ggacgaaatc taaggggctc
     3541 tacgtaacga ccgcgcctgc gccgcatggc attccagagg ctgcgaccca atcgtggatc
     3601 gccgcaagtg gccctctatg acgcaagaca ctcctctcaa gcacgaaggc cgcgcacggt
     3661 tgctgctggt cgaagacgac cccggcgttc gtcgctcgct gcaactgctc ctgcacggac
     3721 gtggctttga ggtgcgcgcc tatgcttcgg gcctggcgct gctggcggac cctacggcgc
     3781 tcgacgcagt gtgtctggtc tctgactacc gcatgcccga gatcgacggg ctattgatcc
     3841 tga
//
LOCUS       DAOMEO010000167         3841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2768_length_3841_cov_2.385895, whole genome shotgun sequence.
ACCESSION   DAOMEO010000167 DAOMEO010000000
VERSION     DAOMEO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2768_length_3841_cov_2.385895"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLH31_05045"
     CDS             <1..243
                     /locus_tag="PLH31_05045"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518835.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQR88627.1"
                     /translation="FPLVVHGAMLVEPTETESRQELDRFVEALRALAGAAKAGDVERF
                     KGAPFHAPMRRLDETLAARKPRLRWKPVAEAPLAAE"
     gene            376..786
                     /locus_tag="PLH31_05050"
     CDS             376..786
                     /locus_tag="PLH31_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88628.1"
                     /translation="MNVSVTIAMPRARQSPADDLAREIVARVLRVVLVVIGLLLIAGG
                     ALIAPLPGPLGLPLTVVGLMLVLRNSFKARKQFVRFQHAHPKLIFPIRRLLRREPEVI
                     LVAWQQALRIERLVIPRRWRVAVRWRKSFKRRGR"
     gene            complement(1040..1549)
                     /locus_tag="PLH31_05055"
     CDS             complement(1040..1549)
                     /locus_tag="PLH31_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQR88629.1"
                     /translation="MEHQSLFDLSNPEFWVLVALVLFFGLLVVLKVLPGALFSALDAH
                     AAKIKAELDEAQHLREEAQTLLADVKAQREEAERQAAGMIEAAKADAKRLAEEAKEKL
                     EEQIKRRAEMAERKIAQAEAQATADVKAAAVDLAAQTAEAVLTARLAGLKSDPLVDAA
                     IGQMASKLQ"
     gene            complement(1562..>2076)
                     /locus_tag="PLH31_05060"
     CDS             complement(1562..>2076)
                     /locus_tag="PLH31_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88630.1"
                     /translation="VETTGAPEAHGGGGLPQLQFEHWGGQIVWLLLIFAVLYVVLSKG
                     LLPRVSGAIETRGAKIAGDIADARRLKDEAEVQARAAAAEVAEARASAQRTAADAKAK
                     ASASAAERQAKEEAVLAEKLAAAEAGIQAARDQAMSHVAAVAQDTASAIVEKLTGKTA
                     SAAELKAALA"
     assembly_gap    2077..2086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2139..2363)
                     /locus_tag="PLH31_05065"
     CDS             complement(2139..2363)
                     /locus_tag="PLH31_05065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HQR88631.1"
                     /translation="MEAEAAKYIGAGLAMLGMIGAGIGLGVMFGNYFQGALRNPTAAA
                     QERPMLFLGMALTEALGIFALVIAFLILFS"
     gene            complement(2410..3171)
                     /locus_tag="PLH31_05070"
     CDS             complement(2410..3171)
                     /locus_tag="PLH31_05070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQR88632.1"
                     /translation="MADPMHQFQVSKIVELPTVTVFGQAIDLSITNSVAAMMLAATLI
                     IVFYAVASAKQAVVPGRLQTVGEMLYGLVNGLTESIIGHDGKKYLPFVFTLFIFVLFA
                     NVQGMFLVFTATSQIAVTLTLGLMVILTVVGVGFAKNGLKFFKLFAPSGVPWPLYFLL
                     VPIEILSFLIRPITLALRLFGNMLGGHVVLKIFAGFVVALGGMGVLGWAGAALALSSV
                     VALTALEFMVAFLQAFVFAVLTCVYLNDVVHLDSH"
     gene            complement(3177..3557)
                     /locus_tag="PLH31_05075"
     CDS             complement(3177..3557)
                     /locus_tag="PLH31_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQR88633.1"
                     /translation="MPKADEPNDKAFQSLDARLDAFEARKAAEQPMRAEHEKSSQDGY
                     RLLADLIGGVLVGLGLGWGLDQFVGTAPFGMVGGLLIGMGASIFSVVRKATKLSAQAS
                     TQAPKEQVGEDTGGGSTAPKQKRD"
BASE COUNT          672 a         1228 c         1242 g          689 t
ORIGIN      
        1 tttccgctcg tcgtccacgg cgcgatgctg gtggagccga ccgagaccga gagccggcaa
       61 gagctggatc gctttgttga agcgcttcgc gccctggccg gcgcagcaaa ggccggcgat
      121 gtcgaacgct tcaaaggcgc gccgttccac gcccctatgc ggcgcctcga cgaaaccctc
      181 gcggcccgca agcctcggtt gaggtggaaa cctgtggctg aagcgccact ggctgccgaa
      241 tagcaggcga ctatttccag acgcggcgaa agggcgcctt tacggcgccc ttatcgatcc
      301 acatatcagg ctcacgcgtc gggctgattg gattttccag ggagcaaacg gagcgtgaag
      361 tccgttagcc ccctcatgaa tgtctcagtc accatcgcca tgccgcgcgc acgccagtcc
      421 cctgccgatg atctcgcccg cgagatcgtg gcgcgcgtcc tacgcgtcgt cctcgtggtt
      481 attggcctgt tgctgatcgc gggcggcgcg ttgatcgcgc ctttgccggg tcccctgggt
      541 ctgccgctga cggtggtggg gctgatgctg gtgctgcgca attcgttcaa ggcccgcaag
      601 cagttcgttc gcttccagca cgctcacccc aagctgatct ttccgattcg ccggctgctg
      661 cgccgcgagc ccgaggtgat cctggtggcc tggcagcagg ctctccgtat cgagcgactg
      721 gtcattcccc gccgctggag ggttgccgtg cgctggcgca agtcgttcaa gcgccggggg
      781 cgctgaggca ccaacctggc gtctgctgga accgcgccgc acggatacac agcgcagctt
      841 tgcccgcagc gatcgggtgt gctggcgggc gttgggccct tcggtgattt cggatccagt
      901 ccggcaaggc cttgctcgtc gtccgctcct gcagcgcctc tgcggccagc cgccgtccag
      961 ggcgaaaagt ctcgacccaa agaaaaagcg cgcgacccgg aggccgcgcg cttgatcatt
     1021 cagccgctat tagtggcttc tattgcagct tggacgccat ttgaccgatg gcggcatcta
     1081 ccagaggatc gcttttcaga cccgccagac gggcggtcag taccgcctcg gcagtctggg
     1141 ccgcgaggtc gaccgcggcc gccttgacgt cggcggtcgc ctgggcttca gcctgggcga
     1201 tcttgcgttc ggccatttcg gcgcggcgct tgatctgctc ttccagcttt tccttggctt
     1261 cctcagccag acgcttggcg tcggccttgg cagcctcgat catgccagcc gcttgacgct
     1321 cggcttcctc gcgttgcgcc ttcacgtccg cgaggagggt ttgggcctct tctcgcaggt
     1381 gctgggcttc atcgagttcg gccttgatct tggcggcgtg agcgtcgagc gccgagaaca
     1441 gggcgcccgg caggaccttg agcacgacca gcaggccgaa gaacaggacc agagcgacca
     1501 gaacccagaa ctcggggttg gagaggtcaa acagggattg atgttccatc gtctgttctc
     1561 cttaggccag agcggctttg agctcggcgg ccgaagccgt cttgcccgtc agcttctcga
     1621 cgatggcgct ggccgtatcc tgggccacgg cggcgacatg gctcatggcc tggtcgcgcg
     1681 cggcctggat cccggcctcg gcggcggcca gcttttcagc caacacagcc tcttccttcg
     1741 cctggcgttc ggcggccgag gccgaagcct tggccttggc gtctgctgca gtccgctggg
     1801 ccgaggcgcg agcctcggcg acctcggccg cagccgctcg ggcctgaacc tcggcttcgt
     1861 ccttcagacg acgggcgtcg gcgatgtcgc cggcgatctt ggcgccgcgc gtctcgatcg
     1921 cgccgctgac ccggggcagc agccccttgg acagcaccac gtagagaaca gcgaagatca
     1981 gcagaagcca gacgatctgg ccgccccagt gctcgaactg cagctgcgga agtccgccgc
     2041 caccgtgagc ctcgggcgcg ccagtcgttt cgactgnnnn nnnnnnaatc aaggactcgg
     2101 agcgagctgc ttccgtggga agcggctcgc gccggggttt aggagaacag gatcaggaag
     2161 gcgataacca gggcgaagat gcccagggct tccgtcaggg ccatgcccag gaacagcatc
     2221 ggacgctctt gagcggcagc ggtcgggtta cgcagagcgc cttggaaata gttaccgaac
     2281 atcacgccca ggccgatgcc cgcgccgatc atgcccagca tcgccagacc agcgccgatg
     2341 tacttcgcgg cttcagcttc cattgtgaga ctcctaaaga tcgaaaggtt ggatagggaa
     2401 aaagggtgat cagtggctgt cgaggtgcac gacgtcgttc agatagacgc aggtgagcac
     2461 cgcaaacacg aaggcctgaa ggaaggccac catgaactct agagccgtca gggcgacgac
     2521 ggaggagagg gccagcgccg cgccggccca gccgagcacg cccatgccgc cgagggcgac
     2581 gacgaagccg gcgaagatct tcagcaccac gtggccgcca agcatgttgc cgaacagacg
     2641 cagggccagg gtgatggggc ggatcaggaa cgacaggatc tcgatcggca ccagcaggaa
     2701 atacagcggc cacggcacgc ccgacggcgc gaacagcttg aagaacttca ggccgttctt
     2761 ggcgaagccg acgccgacca cggtcaggat gaccatcagg ccaagcgtca gggtcacggc
     2821 gatctgcgag gtcgcggtga acaccaggaa catgccctgg acgttggcga acagcacgaa
     2881 gatgaacagg gtgaagacga agggcaggta cttcttgcca tcgtgaccga tgatcgactc
     2941 ggtcaatccg ttgactagtc cgtacagcat ctcgcccacc gtctgcaggc gacccggcac
     3001 cacggcctgc ttggccgagg ccacggcgta gaacacgatg atcagggtcg cggccagcat
     3061 catggcggcc acggaattgg tgatcgacag gtcgatcgct tggccgaaca cggtcacggt
     3121 cggcagttcc acgatcttgc tgacctggaa ctggtgcatc ggatcggcca ttggccctag
     3181 tctctcttct gcttgggggc ggtagacccg cctcccgtgt cttcgccgac ctgctctttg
     3241 ggagcttgcg tcgacgcctg cgcgctcagt ttcgtcgcct tgcggacgac ggagaatatc
     3301 gaagccccca tgccgatcag cagaccgccg accatgccaa agggcgccgt tccgacgaac
     3361 tgatcgagtc cccagcctag gccaagcccc accagcacgc cgccgatcaa atccgccaga
     3421 agacgatagc cgtcctggga cgatttctca tgctccgcgc gcatcggctg ttccgccgct
     3481 ttcctggcct caaatgcgtc gagccgagcg tcgaggcttt gaaaggcctt gtcgttcggt
     3541 tcgtcggcct tgggcatggg agcggtcagg tccgaaaacg cctgcggttg cgccaaaagt
     3601 ggagcatttc cggccggctt gatcgcggcg gaacctatag acctcgctgc ggtgcgtcaa
     3661 ggttgcgaga aagtgttcca accaactgtt taataaggtg ttttgccccg cgttgtcgtt
     3721 ccgagggcaa atccttgcag cggcgctgtt caaggcgcgt caggcgggac gaaacggggc
     3781 tccagttccg ccccgtgggc acgaatggtg cgctgcaacg ccgttcgaca ggcttcgcgg
     3841 c
//
LOCUS       DAOMEO010000168         3834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2771_length_3834_cov_2.418100, whole genome shotgun sequence.
ACCESSION   DAOMEO010000168 DAOMEO010000000
VERSION     DAOMEO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_3834_cov_2.418100"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>862)
                     /locus_tag="PLH31_05080"
     CDS             complement(51..>862)
                     /locus_tag="PLH31_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88634.1"
                     /translation="GACSPLSLFATFTPKDAARTPLRGSAYGPGPRQRLDVYAPPNAT
                     DAQPVAIFFYGGSWDSGRRGDYAWAGRALAAQGFLTLVPDYRLYPEVVFPDFLDDGAS
                     AVRWAVDNAAALGGDPQRIVLIGHSAGAYNAAMLALDPRYLKAAGVERSVVRAFAGLS
                     GPYDFLPLDGPITRRTFGAAGDLPATQPGLYAAAGSPAAFLASGDADTTVYPRNTRKL
                     AAALRQAGARVEERHYPGLDHADTVLALSRPFRGKTDLLSQMTAFLKASAL"
     assembly_gap    863..873
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(977..1276)
                     /locus_tag="PLH31_05085"
     CDS             complement(977..1276)
                     /locus_tag="PLH31_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR88635.1"
                     /translation="MDNFEIDRIDEVIHGRVRLGIMAYLSGAEVADFNTLKTRLSVTD
                     GNLSVHLRKLDDAGYVAIEKSFVGRKPLTRVSLTERGRTAFGLYLDAIGKLVEGR"
     gene            complement(1257..1922)
                     /locus_tag="PLH31_05090"
     CDS             complement(1257..1922)
                     /locus_tag="PLH31_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88636.1"
                     /translation="MTEEIKAIRDDIAFLRALAEEGRQTPMLGGGVLAAAGTIFGLAS
                     LAHWATVDRLLAAPPIAPLVIWPGAGVIFAIVARALLRQSRGKIGAKASVNRTTSAAW
                     SAVGWTILAIWMALMAMSYRTGNWAVMEVFPIIILALYGAAWAVAAAMTHKGWMRLTA
                     GGCFTAAIVMGLLAGTPHMLLAHAAGLALFAVVPGLALMRQEPPEMSGRKGLQGADGQ
                     FRD"
     gene            complement(1989..3779)
                     /locus_tag="PLH31_05095"
     CDS             complement(1989..3779)
                     /locus_tag="PLH31_05095"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HQR88637.1"
                     /translation="MRLQARLKREWVFLKGLMRTLKRVKSIAPDSANLICDDLEAAVD
                     RWSGSPAITFEGKTITYAELDAIANRYAHWAKGQSITRGQTVALFMPNRLEYLAIWYG
                     LSKVGVATALINNQLTGPALAHCLNISQALHCIVDPETSPCFEQVRDGLGRHIQQWVL
                     GPAHGDQRDLVNALKSCSQLRPDRETARGGLTANDTALYIFTSGTTGLPKAARITHMR
                     AQLYMRGFAGSTDARHTDRIYVTLPLYHATGGLCAMGAGLLNGGSIVLRKKFSATHFW
                     SDIRTERCTMFVYIGELCRYLANQPASDDDRNHKIRLIFGNGLRPDVWDDMIDRFKVG
                     GVLEFYGATEGNVSLFNFDGRRGAIGRIPGYLKKKFNIKIVKFDVETEMPIRGPDGCC
                     ILVAPGEVGECIGQIANDARSNFTGYADKAATEKKVLHDVFAKGDAWFRTGDLMRTDG
                     DDYIYFVDRIGDTFRWKGENVATSEVSERLAGVPGVLEVNVYGVQIGDLDGKGGMASL
                     VVGPEFDIATLADYVDRELPGYARPIFVRLQREIETTGTFKYRKIDLVTDGFDPAKTR
                     DPLYFRDPAKGYVKITKTVHAKIVAGGFKL"
BASE COUNT          692 a         1255 c         1249 g          627 t
ORIGIN      
        1 tagagccctt ttcgcccgac cctgatgaag ccatcaggtc ggaaagggct ctagagcgcc
       61 gaggccttca ggaacgccgt catctggctc aacagatcgg tcttgccccg gaacggacgc
      121 gacagggcca gaaccgtgtc ggcgtgatcc agccccggat agtggcgctc ctcgaccctc
      181 gcgccggcct ggcgcagcgc ggctgccagc ttgcgggtgt tgcgcggata gacggtggtg
      241 tcggcgtccc ccgaggcgag gaaggcggct ggcgaacctg ccgctgcata gagtccgggc
      301 tgggtggcgg ggagatcacc cgccgcgcca aaggtgcgcc gggtgatggg tccatccagc
      361 ggcaggaagt catagggacc cgacagcccg gcaaaggcgc gcaccaccga tcgctcgacg
      421 ccggcggcct tcagatagcg cgggtccagg gccagcatgg cggcgttgta ggcgccggcc
      481 gaatggccga tcaggacgat gcgttgcgga tcaccgccca gagccgcggc gttgtccacc
      541 gcccagcgca ccgccgacgc gccgtcatcc agaaagtccg gaaagacgac ctcgggatag
      601 aggcggtaat ccggcacgag cgtcaggaag ccctgcgccg ccagggcccg gcccgcccac
      661 gcatagtcgc cgcgccggcc ggaatcccac gagccgccat agaagaatat cgccacgggt
      721 tgggcgtcgg tcgcgttcgg cggcgcatag acgtccagcc tctggcgggg gccgggtcca
      781 taggccgagc ccctcagggg cgtgcgggcc gcgtccttgg gcgtgaaggt tgcaaacagg
      841 ctcagcggcg agcacgcgcc cannnnnnnn nnncgtcaag ccgacgcgcc gcgtcattga
      901 accactcgcc acacgtcgct ccgccttttg tcgctgcaga aagatacggg accatcgacg
      961 atccggacga gcgttttcag cggccctcga ccagtttgcc gatggcgtcg agatagagac
     1021 caaacgccgt gcgaccacgc tcggtgaggc tgacgcgcgt cagaggcttg cggcccacaa
     1081 agctcttctc gatggccaca tagccggcgt catccagctt gcgcaggtgg accgacagat
     1141 tgccatcggt caccgatagc ctggtcttca gggtgttgaa atccgcgacc tccgcgccgg
     1201 aaagataggc catgatcccc aagcgtacgc gtccgtggat gacctcatcg atgcgatcaa
     1261 tctcgaaatt gtccatctgc gccttggagc cctttccgac ctgacatctc cgggggctct
     1321 tgccgcatca gggccaggcc cggaaccacg gcgaacagcg ccaggccggc cgcatgggcc
     1381 agcagcatgt ggggcgttcc ggccagcagc cccatgacga tcgccgccgt aaagcagcca
     1441 ccggcggtca gccgcatcca gcccttgtgc gtcatggcgg ccgcaacggc ccaggcggcg
     1501 ccgtagaggg ccaggataat gatcgggaag acctccatca cggcccagtt cccagtgcga
     1561 tagctcatgg ccatcagcgc catccagatc gcaagaatgg tccagcccac cgccgaccag
     1621 gcagcgcttg tcgtgcggtt gaccgaggcc ttcgcgccga tcttgccccg cgactggcga
     1681 aggagcgccc gcgccacgat cgcgaagatc acgcccgcgc caggccagat caccagaggc
     1741 gctataggcg gagcggcgag caggcggtcc acggtcgccc aatgcgccag cgaggccagg
     1801 ccgaagatcg ttccagccgc cgccagcacg ccgccgccca gcatgggagt ctgacgcccc
     1861 tcttcggcga gggctcgcag aaacgcgata tcatcgcgga tggccttgat ctcttccgtc
     1921 atgacacggc tctcccccga tggatggacc gaggggtatc gctgcggctt tgcagagtaa
     1981 agttcttctc agagcttgaa gccacccgcc acgatcttgg cgtggacggt cttggtgatc
     2041 ttcacatagc ccttggccgg gtcgcggaaa tagagcggat cgcgggtctt ggcgggatca
     2101 aagccgtcag tgaccagatc gatcttgcga tacttgaagg tgccggtggt ttcgatctcg
     2161 cgctgcagcc gcacgaagat cggccgggca tagcctggta gctcccgatc gacatagtcg
     2221 gccagggtgg cgatatcgaa ctcgggccca acgaccagcg aagccatacc gcccttgcca
     2281 tccaggtcac cgatctggac gccatagaca ttgacctcca gcacgccggg cacaccggcc
     2341 aggcgctcgg acacctcgct ggtggcgaca ttctcgccct tccagcggaa ggtgtcgccg
     2401 atccggtcga caaagtagat gtagtcgtcg ccgtcggtgc gcatcaggtc gccggtgcgg
     2461 aaccaggcgt cacccttggc gaagacgtcg tgcagcactt tcttttcggt cgcggccttg
     2521 tcggcgtaac cggtgaaatt ggagcgggcg tcgttggcga tctgaccgat gcactcgccc
     2581 acctcgccgg gcgcaaccag aatgcagcac ccgtccggcc cgcggatcgg catctcggtc
     2641 tcgacgtcga acttgacgat cttgatgttg aatttcttct tcagatagcc ggggatgcgc
     2701 ccgatggcgc cccgccgtcc gtcgaagttg aagagcgaga cattgccctc cgtcgcgcca
     2761 tagaactcca gcacgccgcc caccttgaag cgatcgatca tgtcgtccca cacgtcgggg
     2821 cgaagaccgt tgccgaagat caggcggatc ttgtgattgc ggtcatcatc gctcgccggc
     2881 tggttggcca gatagcggca cagctcgccg atatagacga acatcgtgca gcgctcggtg
     2941 cggatatcgc tccagaagtg cgttgccgag aacttcttgc gcagcacgat cgagccgccg
     3001 ttgagcaggc ccgcccccat ggcgcagagg ccgcccgtgg cgtggtaaag cggcagggtg
     3061 acatagatgc ggtcggtgtg acgcgcgtcg gtcgagccgg cgaaaccccg catgtagagc
     3121 tgggcccgca tgtgggtgat gcgcgcggcc ttgggcaggc cggtggtgcc actggtaaag
     3181 atgtagagcg cggtatcatt ggccgtcaga ccgccccgcg cggtctcgcg gtcggggcgc
     3241 aactggctgc agctcttcag cgcattgacg aggtcccgct ggtcgccgtg cgcgggcccc
     3301 agcacccact gctggatgtg ccggcccagg ccgtccctga cctgctcgaa acagggcgag
     3361 gtctccgggt cgacgatgca atgcagggcc tgggaaatgt tcaggcagtg agccagggcc
     3421 gggccggtca actggttgtt gatcagggcg gtggcgacgc cgaccttgga caggccgtac
     3481 catatcgcca gatattccag acggttgggc atgaacagcg ccaccgtctg gccgcgcgtg
     3541 atgctctgcc ccttggccca gtgggcgtag cggttggcga tcgcgtccag ctcggcatag
     3601 gtgatcgtct tgccttcgaa ggtgatcgcc gggctgcccg accagcgatc aaccgccgct
     3661 tcgaggtcgt cgcagatcag attggcgctg tcgggtgcga tggacttcac gcgcttgagc
     3721 gtgcgcatca accccttgag gaacacccac tcacgcttca acctagcctg caaacgcata
     3781 agcaccctcc gttcaaacaa ccattggaga gcgccgcgcg gcgggtcaag acac
//
LOCUS       DAOMEO010000169         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2773_length_3833_cov_2.135786, whole genome shotgun sequence.
ACCESSION   DAOMEO010000169 DAOMEO010000000
VERSION     DAOMEO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2773_length_3833_cov_2.135786"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLH31_05100"
     CDS             <1..992
                     /locus_tag="PLH31_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88638.1"
                     /translation="AIGKIRGVIEVDGGLRIAGDAIVITVNRPAAALEGLDPEAITRQ
                     LGMLTGGSVIGQIQEGDKLVGVRVWTEGSLRDRFRTLEDLPLRAPDGHALPLKRIATL
                     SIAPGQPPVTRESLQPLVAVTARLEGRDLGSAMREVSQTVSRLNLPAGVRVEYGGLYA
                     EQQKSFRDLALVFLSALLLVSLLFLYLFRNWAAVGSIVATLVLAASSVFLGLWVTGTE
                     LNISAMMGLTMVIGIVAELGVFYLAELPAGQRPNSDALIAAGRARLRPILMSALIAIL
                     ALAPLALGLGEGSAMQKPLAIAIITGLLAGVPLILIFTPTLLARLSKPLRERA"
     gene            1330..2472
                     /locus_tag="PLH31_05105"
     CDS             1330..2472
                     /locus_tag="PLH31_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88639.1"
                     /translation="MFMDISSEMIHSLLPVFLVTTLGASAALVGVIEGVAEATASITK
                     VFSGWLSDRLGKRKLLAVLGYGLGAASKPIFPFAITPFEILGARFADRIGKGLRGAPR
                     DALVADIAPPGTRGAAFGLRQSLDSIGAFLGPLVAIVLMLVLAGNIRAVFAWAIVPAV
                     IAVVLLVIGVEEPGRSGEAKRAALPIQWREVADMGRPFWSVVAVGMVFTMARFSEAFL
                     VLRAEDVGLALALVPAVMIVMNVVYAAVAAPVGALSDHIDRRMLLACGLVVLILADLA
                     LALIPTVGGVLLGVLLWGLHMGMTQGLLAALVADAAPDRLRATAFGIFNLASGLTLLL
                     ASTLAGLLWSALGPGATFFAGAAFSAVALMGLAAQPHARRDSVDGR"
     gene            3010..>3833
                     /locus_tag="PLH31_05110"
     CDS             3010..>3833
                     /locus_tag="PLH31_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQR88640.1"
                     /translation="MRIADQTLATVADTAPFPAHAGYELSIIIPTLNERENLRALSQR
                     LECALRGIAWQAIIVDDDSGDGTASAAKALAVLDARFSCLRRIGRRGLAGAVIEGVMA
                     SAAPFIAVLDADLQHDESLLSVMLESVRNGQADVAVASRFVRREDSAVGLSPLRRLGS
                     LAANGLARRILGQSLSDPMSGFFVTRRSVFDETASRLSPQGFKILFDLLATRRGQLSV
                     REFPYAFRPRVAGSSKLDHRVTAEFLGLALAKTTRDLLSPRAAVFLAVGASGLVVHL"
BASE COUNT          550 a         1234 c         1248 g          801 t
ORIGIN      
        1 aagccatcgg caagatccgt ggtgtgatcg aggtcgatgg gggactgcgc attgccggtg
       61 acgccatcgt catcaccgtc aatcgaccgg cggccgcgct cgaagggctc gatccagagg
      121 ccattactcg tcaactcggc atgttgaccg gcggttcagt catcggccag attcaggaag
      181 gcgacaagct ggtcggggtg cgagtgtgga ccgaaggctc attgcgcgac cgcttccgca
      241 ccctggagga cctgccgttg cgcgcgcccg acggtcacgc gctgccgctg aagcgcattg
      301 cgaccttgtc gatcgcgccc ggccagccgc cggtcacccg cgagagcctc cagcctctgg
      361 tcgcggtgac cgcgcgtctc gagggtcgcg acctaggctc cgcgatgcgc gaggtctctc
      421 agacggtgtc gcggctcaac ctgccggccg gagtccgggt cgaatacggc ggtctctacg
      481 cagagcagca aaagtcgttt cgcgaccttg ccctcgtatt tttatcggcc ttgctgctcg
      541 tcagcctcct gttcctctac ctgttccgca actgggccgc agtcggcagc atcgtcgcca
      601 ccctggtcct ggccgcgtcg agcgtcttcc tgggcctttg ggtcacgggc accgagctga
      661 acatctcggc gatgatggga ttgacgatgg tgatcggcat cgtcgccgag ctgggcgttt
      721 tctatctagc cgagctgccg gcaggccaac ggccgaactc tgacgcgctc atcgccgcgg
      781 gccgagcccg tctgcggccg atcctgatgt cggcccttat cgccattctg gccctggcgc
      841 cgctggcctt gggtcttggc gagggctcgg cgatgcagaa gccgcttgct atcgcgatca
      901 ttacaggttt gctcgcgggg gttcctctga tcctgatctt cacgcccacg ctactggcgc
      961 gcttgtccaa gcctcttcgc gagcgcgcct gatgatcccc tttcatcaac gacagcccgg
     1021 acgcgagcgc cgccgtccgg ctgccgagaa caaacccgct tgctccatct ggtattgcgg
     1081 cagaggtgtc gtggccctga cgctcggcca catcggggtg gtcgcggcga ccagagagtt
     1141 ccatccggtc gcgccgctgg ctgccgccgc ccaattgccg tcaccgatcg tgcggcctct
     1201 cgaccgaagc cagtctggag cggctggctt aggggcgccg acgaaatcgg cgccaacagg
     1261 ataggcccga tgataatccg aagattacgg gcggttccgc gcggggtctg ggcgcttggc
     1321 ttcgtgtcca tgttcatgga catctcgtcg gagatgatcc acagtttgct gccggtattc
     1381 ttggtcacca cgctcggcgc cagcgccgcc ctggtcggcg tgatcgaggg tgtggctgag
     1441 gcgacagcgt ccatcaccaa ggtcttctcg ggctggctca gcgaccggct gggcaagcgc
     1501 aagctcttgg cggttctggg ctatggtctc ggtgcagcct caaagcctat ctttcccttc
     1561 gccattacac cgtttgagat cctgggcgcg cggttcgccg atcggatcgg caaaggcctg
     1621 cggggcgcgc cgcgtgacgc cttggtcgcc gatatcgcgc ctccgggaac ccggggcgcc
     1681 gccttcgggc tacgccagtc gctggattca atcggcgcct tcctaggccc cttggtcgcc
     1741 atcgtcctga tgctggtcct ggccggaaac atccgggccg tcttcgcctg ggccattgtt
     1801 cccgccgtga tcgcagtcgt cctgctggtc atcggcgtcg aggagcccgg acggtccggc
     1861 gaggcaaaaa gagcggcgct tcctattcag tggcgcgagg tggccgacat gggacggccg
     1921 ttctggtctg tggtcgcggt cggaatggtc tttaccatgg cgcggttcag cgaggcgttt
     1981 ctcgtcctgc gggccgagga tgtcgggctc gcgctcgcgc tggttccggc cgtcatgatc
     2041 gtcatgaatg tcgtctacgc agcagtcgcc gcacccgtgg gcgccctgtc ggaccatatt
     2101 gatcggcgga tgctgctagc ttgcgggctg gtggttctga tccttgcgga cttggctctc
     2161 gccctgatcc cgaccgtggg gggcgtcttg ctcggcgtcc tcctgtgggg cctgcacatg
     2221 ggcatgaccc agggacttct ggccgcgctg gtagccgacg ccgcgccgga ccggcttcgg
     2281 gctacggcct tcgggatctt caacctcgct tcgggcctga ccctcctgct ggccagcacg
     2341 ctcgccggac tgctctggtc ggcccttggc ccgggcgcta cctttttcgc aggcgcagcc
     2401 ttttccgccg tcgccctaat gggcctcgcc gcgcagcccc acgcccggcg agattccgtt
     2461 gacggcagat gacgccggga gccgacctgc ttgggaacct tgaggtcgtc cggttttcac
     2521 cgcattgcag ggctcgtccc ggaggacccg cctaaattga ccccgcgatg gataaggctc
     2581 cgcgttcaac cgccccaagt cgttggtggc tccgcctcga aagaccgttg ttgatcatcg
     2641 tggcgatggc tgcgggcaag gtctggcgct tcctctgtct cgctgacgag gtttgcaaga
     2701 gcgggatttg cagccctcgg cggcttcaca gcgctatccc tgatcagcgt gttcgcccag
     2761 gccatgctag ttgtccatag gcggcgcgcc taaggcggcg atattcttag gcgatattct
     2821 tagcggcggt ctgcgaggcg caggtcgtct ccgaaggcgc caagtttctg gttttccggc
     2881 gtcggcccga catcagccgg cacctagacc tcacctattc cagcaacttt cataattctg
     2941 tgaggcgcct ggcgtggcgt gacgtctttc gcagcagcga caacatcacg tcgcagccgg
     3001 gcgaatagaa tgcgcattgc cgatcagacc ctggctaccg tagcggatac ggcccccttt
     3061 cctgcccacg caggctacga gctatcgatc attatcccga cgctgaacga acgggagaac
     3121 ttgcgcgcgc tatcgcagcg gctggagtgc gcgctgcggg gtatcgcgtg gcaggcgatc
     3181 atcgtcgatg acgattctgg ggacggaaca gcctccgccg cgaaggccct agccgttctc
     3241 gacgcacgct tttcatgtct gcggcgcatt ggacggcgag ggctggcggg cgccgtgatc
     3301 gagggggtca tggccagcgc cgcgcctttc atcgctgtgc tcgacgcgga tcttcaacat
     3361 gatgaaagct tgctctcggt gatgctggaa agcgttcgga atggccaggc agacgttgcc
     3421 gtggccagcc gatttgtgcg ccgcgaagat agtgcggtgg gcttgtcgcc tttgcgccgc
     3481 ctcggcagtc tggcggccaa cggcttggcg cgtcgtattt tgggtcagag tctatccgat
     3541 ccgatgagcg gatttttcgt cacgcggcgt tcggtgtttg acgagaccgc gtcacggctg
     3601 tcgccgcaag gcttcaagat tctgttcgat ctgctggcca cgcgtcgcgg tcaattgtcc
     3661 gtgcgcgagt ttccctatgc gttcaggccc cgcgtagcag gatccagcaa actcgatcat
     3721 cgggtcacgg ccgagttcct cggcctggct ctggcgaaga cgacgaggga cctcctctcc
     3781 ccgcgtgcgg cggtgtttct ggcggtgggt gcaagcggac tagtcgttca tct
//
LOCUS       DAOMEO010000170         3831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2778_length_3831_cov_2.404661, whole genome shotgun sequence.
ACCESSION   DAOMEO010000170 DAOMEO010000000
VERSION     DAOMEO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2778_length_3831_cov_2.404661"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..618
                     /locus_tag="PLH31_05115"
     CDS             22..618
                     /locus_tag="PLH31_05115"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQR88641.1"
                     /translation="MILVIDNYDSFTYNLVHYLNELGAETLVHRNDDLTVQQALALRP
                     AAILLSPGPKAPDQAGICLPLLRGAPEDMPILGVCLGHQAIGQAYGGEVIRAKEVMHG
                     KTSPIRHADKGIFKGLPNPFTATRYHSLAIRREDLPPELEVTAWTDDGEIMGVQHRTR
                     PVHGVQFHPESIATEGGHQMLANFLDLAGVKRDAAIWV"
     gene            628..>1093
                     /locus_tag="PLH31_05120"
     CDS             628..>1093
                     /locus_tag="PLH31_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQR88642.1"
                     /translation="MSDTFKPLLAKLADGQTLSEDDAEVFFSACLRGEPTPAQVGAAI
                     TAMRLRGETVGEIAACARAMRRAAVMLEHPYDVIDVCGTGGDGLHTLNISTAVGFVAA
                     GGGLKVAKHGNRAVTSKSGTADVLAALGVNIDASRDQQRKALDEAGICFLFAQ"
     assembly_gap    1094..1103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1104..1663
                     /locus_tag="PLH31_05125"
     CDS             <1104..1663
                     /locus_tag="PLH31_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQR88643.1"
                     /translation="HSDHAGCRGHTMNHFYDGIFNLLGPLTNPAGARRQVVGVSAPRF
                     VEPIARALGALGAERAWSVHGQGMDELTTTGETEVAEWRHGAVRLFTITPEAVGLPRA
                     SLADLTGGEPDFNAAALTRLLDGEKGAYRDIVLLNAAAAFLVADKVETLSEGIKAAAA
                     SIDSGAAKAALAGLVAATNTELASA"
     gene            1660..2448
                     /gene="trpC"
                     /locus_tag="PLH31_05130"
     CDS             1660..2448
                     /gene="trpC"
                     /locus_tag="PLH31_05130"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQR88644.1"
                     /translation="MTDILAKIAAYKRDEVVDRKRRRTLTALENAAKNASSPRGFKAA
                     LKNAHAPGKLALIAEIKKASPSKGLIRADFDPPALARAYEAGGAACLSVLTDGPSFQG
                     ADPYLIDARAAVSLPCIRKGFLVDPWQVVESRALGADAILVILAMIDDVLAAELMSEA
                     ERLDMDALVEVHDEAEMARAGQLGATLVGINNRDLKSFVVDLAVTERLAAQAPTDALL
                     VTESGLFTHDDAVRMEAAGATAMLVGESLMRQANVAEATRVLLG"
     gene            2572..3117
                     /locus_tag="PLH31_05135"
     CDS             2572..3117
                     /locus_tag="PLH31_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR88645.1"
                     /translation="MNRSLVAFFKHEGWTIEGAAPTATRKFVIVAAPHTSNWDFVYFL
                     GAADALNLKLSFMGKASLFRWPFARLMRDLGGVPVDRSRSGDTVQAMADEFARREEFM
                     LTIAPEGTRGKARQWKTGFYNIALKAGVPLVPAMMDYRRKVVGLGQAIMPSGDYDKDL
                     AQLAAFYRTCTPKHPERATSL"
     gene            complement(3171..3521)
                     /locus_tag="PLH31_05140"
     CDS             complement(3171..3521)
                     /locus_tag="PLH31_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88646.1"
                     /translation="MDAQGFVDVKLTTQERKGLDNRWWRTIFRGRLVMSFQQLNPTMP
                     VIVEGKGSGFAFAVIDYGQEHHLIWVVAIDGTGEIWSAPNPKVRVQSNWTMGRKIAPP
                     ANIESLRTAAAAAG"
BASE COUNT          667 a         1357 c         1161 g          636 t
ORIGIN      
        1 gcccgttcta gaagctaggt catgatcctg gtcatcgata actacgacag ctttacctac
       61 aacctcgttc actatctgaa cgagctgggg gcggagacgc tcgtgcatcg caacgacgac
      121 ctgacggtgc agcaggccct ggccctgcgc cccgccgcca tcctgctgtc gcccggcccc
      181 aaggcccccg accaggccgg catctgcctg cccctgctgc gcggcgcacc ggaagacatg
      241 ccgatcctgg gcgtgtgcct gggccaccag gccatcggcc aggcctatgg cggcgaggtg
      301 atccgcgcca aggaagtgat gcacggcaag accagcccga tccgccatgc cgacaagggc
      361 atcttcaagg gtctgcctaa cccctttacc gccacgcgct atcacagcct ggccatccgc
      421 cgcgaagacc tgccgcccga gctggaggtc accgcctgga ccgacgacgg cgagatcatg
      481 ggcgtgcagc accgcacccg cccggtccac ggcgtacaat tccaccccga atccatcgcc
      541 accgagggcg gccatcagat gctggccaac ttccttgacc tggccggcgt caagcgcgac
      601 gcggcgatct gggtctaggc ttcaaccatg tccgacacct tcaagccgct gctggccaag
      661 ctggccgacg gccagaccct gtccgaggac gacgccgagg tcttcttcag cgcctgcctg
      721 cgcggcgagc ccaccccggc ccaggtcggc gcggcgatca ccgccatgcg cctgcgcggc
      781 gagacggtgg gcgagatcgc cgcctgcgcc cgcgccatgc gccgcgccgc cgtcatgctg
      841 gagcaccctt acgacgtcat cgacgtctgc ggcaccggcg gcgatggcct gcatacgctc
      901 aacatctcca ccgccgtcgg cttcgtcgcg gcgggcggcg ggctgaaggt ggccaagcac
      961 ggcaaccgcg ccgtgacctc caagtccggt acggccgacg ttctggccgc cctgggcgtc
     1021 aatatcgacg caagtcgcga tcagcagcgc aaggctctgg acgaggccgg catctgcttc
     1081 ctgttcgccc aggnnnnnnn nnntacatag cgaccacgcc ggttgccgag gacacacgat
     1141 gaaccacttt tacgatggca tcttcaacct gctgggcccg ttgaccaatc cggccggcgc
     1201 ccggcgccag gtggtgggcg tctcggcccc gcgcttcgtc gagcccatcg cccgcgccct
     1261 cggtgcgctc ggagcagagc gcgcctggtc agtgcacggc cagggcatgg acgagctgac
     1321 caccaccggc gagaccgagg tcgccgaatg gcgccatggg gcggtgcgcc tcttcacgat
     1381 cacgcccgag gccgtcggtc tgccgcgagc ctcgctagcc gacctgaccg gcggcgagcc
     1441 cgacttcaac gccgccgccc tcacccgcct gctggacggt gaaaagggcg cctatcgcga
     1501 catcgtgctg ctgaacgccg cggccgcctt cctggtcgcc gacaaggtcg agaccctcag
     1561 tgagggcatc aaggccgccg cagcctccat cgacagcggc gcggccaagg cggctctggc
     1621 cggtctggtc gcggccacca ataccgaact ggcgagcgca tgaccgacat cctggccaag
     1681 atcgccgcct acaagcgcga cgaggtcgtc gaccgcaagc gccgccgcac cctgaccgcg
     1741 ctcgagaacg ccgccaagaa tgcctcgtct ccgcgcggct tcaaggctgc cctgaaaaac
     1801 gcccacgccc ccggcaaact ggccctgatc gccgagatca agaaggcctc accctccaag
     1861 ggccttatcc gcgccgattt cgacccacca gcgctggctc gcgcctacga agccggcggc
     1921 gcggcctgtc tgtccgtgct gaccgatgga cccagcttcc agggcgccga tccctatctg
     1981 atcgacgccc gcgccgccgt cagcctgccc tgcatccgca agggcttcct ggtcgatccc
     2041 tggcaggtgg tcgagagccg cgccctgggc gccgacgcca tcctggtgat cctggcgatg
     2101 atcgacgacg tcctggccgc cgagctgatg agcgaagccg aacgcctgga catggacgcc
     2161 ctggtcgagg tgcatgacga ggccgagatg gcccgcgccg gccagttggg cgccaccctg
     2221 gtcgggatca acaatcgcga cctgaagagc ttcgtcgtag acttggccgt cacagagcgc
     2281 ctggccgccc aggccccgac ggacgccttg ctggtcaccg agagcggtct tttcacccac
     2341 gacgacgcgg tgcggatgga agcggccggg gccacggcca tgctggtggg cgaaagcctg
     2401 atgcgccaag ccaatgtcgc cgaggctacc cgcgtcctcc tcggctgaaa ccggaccggg
     2461 tagagctcgt atggcgtttc ggcgtcgcgc catccacagt ctgctttaac cggcccatca
     2521 agacggctgg aatcactcgg gaggtctcga atgcactgga tcgcccgggt cgtgaatcgg
     2581 tccctcgtag ccttcttcaa gcacgaaggc tggaccatcg agggcgccgc cccgacggcg
     2641 acccgcaagt tcgtgatcgt cgccgcgccc cacaccagca actgggattt cgtctacttc
     2701 ctcggcgcgg ccgacgcgct gaacctcaag ctcagcttca tgggcaaggc ctcgctgttc
     2761 cgttggccct tcgcgcgcct gatgcgcgac ctgggcggcg tgccggtcga ccggtcccgc
     2821 tcgggcgaca ccgtccaggc catggccgac gaattcgcgc gtcgtgagga attcatgctg
     2881 acgatagcgc cggaaggcac gcgcggcaag gctcgccagt ggaagacagg cttctacaac
     2941 atcgccctca aggccggcgt gcccctggtg cccgccatga tggactatcg gcgcaaggtc
     3001 gtgggtctgg gccaggcgat catgccttca ggcgactatg ataaggacct ggcgcaattg
     3061 gccgccttct atcgcacctg cacgcccaag catcccgagc gggcgacaag cctctaatcg
     3121 acctaccccc ggcgctgtcg aatcacgccg cgatcggcga tccccggacg tcatcccgcc
     3181 gcagcggccg ccgttcgcag tgattctatg ttggcgggtg gcgcaatctt tcggcccatg
     3241 gtccagttgg actggacccg aaccttggga ttgggcgccg accagatctc gccggtgccg
     3301 tcgatcgcca ccacccagat cagatgatgt tcctgcccgt agtcgatgac cgcaaaggca
     3361 aaaccgctgc ccttgccctc gacgatgacc ggcatggtcg ggttcagttg ctggaagctc
     3421 atcaccaggc gtcctctgaa aatcgtccgc caccaacgat tgtcgagccc cttgcgttcc
     3481 tgcgtcgtca acttgacatc aacaaagccc tgagcgtcca ccaatgattt caattactta
     3541 gaaatcgatc atttccgact atccagttta tcgcgaccct tagtcggaag cgttctggat
     3601 atgcgggctg tcacacgcat tttagcgggg acaaggtcgg atcggcgatc atcgatgtag
     3661 atgaagctcg aacgtggacc ttcactccgg taccggcgtc gagatcaagc cgatcctgcc
     3721 gcgcattttt ccagttgtgt ctcagtttga actctgatgc cgttgacaca cttgacccat
     3781 gtcccatttt cccgcatcaa ttgatgacgg gaaatgggac atcgattcgt g
//
LOCUS       DAOMEO010000171         3820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2790_length_3820_cov_2.679150, whole genome shotgun sequence.
ACCESSION   DAOMEO010000171 DAOMEO010000000
VERSION     DAOMEO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_3820_cov_2.679150"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLH31_05145"
     CDS             <1..351
                     /locus_tag="PLH31_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HQR88647.1"
                     /translation="AKAPATEGSIGVNGYLWRATLDTLAFMPLASADPYGGVIVTDWY
                     VNPEKPDERFKATVYILDTRLRADGLNVSVFKQAKDAAGGWTDTPAADQTATDIENAI
                     LTRARQLRLSNIRG"
     gene            492..3098
                     /gene="leuS"
                     /locus_tag="PLH31_05150"
     CDS             492..3098
                     /gene="leuS"
                     /locus_tag="PLH31_05150"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQR88648.1"
                     /translation="MARYNPKDTEPKWRNAWADADVFKTADISDGRPKYYVLEMFPYP
                     SGRIHMGHVRNYAMGDVVARYKRAQGFSVLHPMGWDAFGMPAENAAMERGVHPKGWTY
                     DNIAAMREQLKALGLSIDWSREFATCDPDYYGKQQAWFLKLLKRGLVYRKEASVNWDP
                     VDMTVLANEQVIDGKGWRSGAAVEKRKLTQWFLRITDYADALIDGLKTLDRWPEKVRI
                     MQENWIGRSKGLRFTFKFANNDNGSEAPDGHAAGLEVYTTRPDTLFGASFVGIAPEHP
                     LAEQLAVENPDLQAFIAECRKGGTSEADIEGAEKLGRDTGLRVVHPLDPSITLPVWIA
                     NFILMDYGTGAIFACPAHDQRDLDFARKYDLPVLPVVLPPGEDPATFAVGKEAYVGPG
                     SIFKSQFLDGMDIESAKSEVVARIEALDQGQGATVYRLRDWGVSRQRYWGCPIPIIHC
                     EACGPVPVPEDQLPVVLPDDVTFEADKPGNPLVRHPTWKHTTCPSCGAPAQRETDTLD
                     TFMDSSWYFARFANPDAAECIDKASADHWMPVDQYIGGVEHAILHLLYARFITRALKD
                     EGLLSVEEPFAGLFTQGMVTHEAYKNAAGEWVEPSDVVVTAEGNSRTAKHATTGELIT
                     IGDIEKMSKSKKNVVAPEDIFEAYGVDAARLFVMSDSPPERDVQWTNSGVEGSWRFTH
                     RLWNEFDSQPAGDFAHDAADEAAVALRKSAHKLIGFVTDSIEGFRFNSGVARLYEFLN
                     ALKAAPAEGASQGVLAARKEALHILARLVAPFTPHLAEEAWASLGGEGLVVDAPWPKA
                     DPALAADDERVLPIQINGKRRGEIKVKAGLSEGEVEKIALADPAVLAHLEGVTVRKVI
                     VVKDRIVNIVAN"
     gene            3102..3605
                     /gene="lptE"
                     /locus_tag="PLH31_05155"
     CDS             3102..3605
                     /gene="lptE"
                     /locus_tag="PLH31_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQR88649.1"
                     /translation="MKRIFLVGALAVSTLGLSACGFTPLYATPGVTPGLASIQTEAPD
                     GRAGFLLREQLDDAFVYNTGAPAAYRLTLAINEQRYARGVRVDNVANRYELRMVVNWR
                     LLDARTGAEVRKGRTDAAVTYDSADQPYSAIAAQNDGQERAAAEVARQIQLDLAAWMA
                     GGRKAKT"
     gene            3630..>3820
                     /locus_tag="PLH31_05160"
     CDS             3630..>3820
                     /locus_tag="PLH31_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQR88650.1"
                     /translation="MILSKRPDVERFLKDPAPDIRAVVIYGKDRGVVRDRADQLAKRL
                     FARPDDLFDTAQLTESDVES"
BASE COUNT          667 a         1346 c         1219 g          588 t
ORIGIN      
        1 gccaaggcgc cggccaccga gggctcgatc ggcgtcaacg gctacctgtg gcgcgctacg
       61 ctcgacaccc tggccttcat gccgctggct tcggccgacc cctacggcgg cgtgatcgtc
      121 accgactggt atgtgaaccc ggaaaagcct gacgagcgct tcaaggcgac cgtctacatc
      181 ctcgacaccc gcctgcgcgc cgacggtctg aacgtcagcg tgttcaagca ggccaaggac
      241 gcggcgggcg gctggaccga taccccggcc gccgaccaga cggcgaccga catcgaaaat
      301 gcgatcctga cccgcgcccg tcagcttcga ctgtccaaca tccggggcta aggcctccgg
      361 caggccttcg ccgtcacccg gcgaaggcct gacccccgca ctttccagcg cgctccggcc
      421 gcgaaaccgc ttatatgtct tgcggacgcg cctgcccggg cgcctttcgc atatccagac
      481 atccggtttt catggcccgc tacaatccca aagacaccga gcccaaatgg cgcaacgcct
      541 gggcggacgc cgacgtcttc aagacggcgg acatctcgga tgggcggccc aaatactatg
      601 tgctcgagat gttcccgtac ccgtcgggcc gcatccacat gggccatgtg cgcaactatg
      661 cgatgggcga cgtggtggcc cgctacaagc gcgcccaggg cttcagcgtc ctgcacccga
      721 tgggctggga cgcctttggc atgccggccg aaaacgccgc catggagcgc ggcgtccacc
      781 ccaagggctg gacctacgac aacatcgcag cgatgcgcga gcagctgaag gccctgggcc
      841 tgtcgatcga ctggtcgcgc gagttcgcca cctgcgaccc ggactactat ggcaagcagc
      901 aggcctggtt cctgaagctg ctgaagcggg gcctggtcta tcgcaaggag gcctcggtca
      961 actgggaccc ggtcgacatg accgtgctgg ccaacgagca ggtcatcgac ggcaagggct
     1021 ggcgctcggg cgcagccgta gagaagcgca agctgaccca gtggttcctg cgcatcaccg
     1081 actatgccga cgccctgatt gatggcctca agaccctgga tcgctggcct gaaaaagtcc
     1141 ggatcatgca ggagaactgg atcggccggt ccaagggcct gcgcttcacc ttcaagttcg
     1201 ccaataacga caatggcagc gaggcccccg acggccatgc cgccgggctg gaggtctaca
     1261 ccacccgtcc cgacaccctg tttggcgcca gtttcgtggg catcgcgccc gagcatccgc
     1321 tggccgagca gctggcggtc gagaaccccg acctgcaggc cttcatcgcc gaatgccgca
     1381 agggcggcac gtcggaagcc gatatcgagg gcgccgagaa gctgggccgc gacactggcc
     1441 tgcgcgtcgt ccacccgctg gacccgtcga tcaccttgcc cgtctggatc gccaatttca
     1501 tcctgatgga ctacggcacg ggcgcgatct tcgcctgccc cgcccacgac cagcgcgacc
     1561 tggatttcgc ccgcaagtac gacctgccgg ttttgcccgt ggttctgccg cccggcgagg
     1621 atccggccac gttcgcggtc ggcaaggagg cctatgtcgg ccccggttcg atcttcaagt
     1681 cgcagttcct ggacgggatg gacatcgagt ccgccaagtc cgaggtggtc gcccgcatcg
     1741 aggccctgga ccagggccag ggcgcgaccg tgtatcgcct gcgcgactgg ggcgtcagcc
     1801 gccagcgcta ttggggttgc ccgattccga tcatccactg cgaggcctgc ggtcccgtgc
     1861 ccgtgccgga agaccagctg ccggtggtgt tgcccgacga cgtcaccttc gaagccgaca
     1921 agccgggtaa cccgctggtg cgtcacccga cctggaagca cacgacctgc ccgtcgtgcg
     1981 gggccccggc ccaacgcgag accgacacgc tggacacctt catggacagc agctggtact
     2041 tcgcccgctt cgccaatccc gacgccgccg agtgcatcga caaggcgtcg gccgatcact
     2101 ggatgcccgt cgaccagtat atcggcggcg tcgagcacgc gatcctgcac ctgctgtacg
     2161 cccgcttcat cacccgcgcc ctgaaggacg agggcctgct gtcagtcgaa gagccgttcg
     2221 ccggcctgtt cacgcaaggc atggtcaccc acgaggccta caagaacgcc gccggcgaat
     2281 gggtcgaacc ctcggacgtc gtcgtcacgg ccgaaggcaa cagccgcacc gccaagcacg
     2341 ccaccaccgg cgagctgatc accatcggcg acatcgagaa gatgtcgaag tccaagaaga
     2401 acgtcgtcgc gcccgaggac atcttcgagg cctacggcgt cgacgccgcg cgcctgttcg
     2461 tgatgagcga cagcccgccc gagcgtgacg tgcagtggac caacagcggc gttgaaggct
     2521 cctggcgctt cacccaccgc ttgtggaacg agttcgacag ccagccggcc ggcgatttcg
     2581 cccatgacgc cgccgacgag gccgcagtgg ccctgcgcaa gtcggcccac aagctgatcg
     2641 gctttgtcac cgacagcatc gagggcttcc gcttcaacag cggcgtggcg cgcctgtacg
     2701 agttcctgaa cgccctgaag gctgctccag ccgaaggcgc atcgcagggc gtgctggcgg
     2761 cccgcaagga ggccctgcac atcctggcgc gcctggtggc cccgttcacg cctcacctgg
     2821 ccgaggaagc ctgggccagc ctgggcggcg aaggtctggt ggtcgacgcc ccctggccca
     2881 aggccgaccc cgccctggcc gctgacgacg aacgcgtgct gccgatccag atcaacggca
     2941 agcgtcgcgg cgagatcaag gtcaaggcgg gtctctcgga aggcgaggtc gagaagatcg
     3001 cgctggctga tccggccgtt ctggcccacc ttgagggggt cacggtccgc aaggtgatcg
     3061 tggtcaagga ccgtatcgtc aacatcgtgg ccaactgacc gatgaaacgc attttcctgg
     3121 ttggcgcgct cgccgtctcc accctcggcc tctcggcctg tggcttcacg ccgctgtacg
     3181 cgacacctgg cgtcacgccg ggcctggcgt cgatccagac ggaagctccg gacggccgcg
     3241 cgggtttcct gctgcgcgaa cagctggatg acgctttcgt ctacaacacc ggcgcgccgg
     3301 cggcgtaccg cctgaccctg gcgatcaatg agcagcgcta tgcgcgcggc gtgcgggtcg
     3361 acaatgtggc caaccgctac gagctgcgga tggtggtcaa ctggcgcctg ctcgacgcca
     3421 ggaccggcgc tgaggtccgc aagggtcgca ccgacgctgc ggtgacctac gactcggccg
     3481 accagcccta ttcggccatc gccgcccaga acgacggcca ggaacgcgcg gcggccgagg
     3541 tggcccgtca aatccagctg gacctggccg cctggatggc tggcggccgc aaggccaaga
     3601 cctgacgcgc cccctgaagg cctgacgcca tgatcctgtc caagcggccg gacgtcgaac
     3661 gcttcctgaa ggatccggcc ccggatatcc gcgccgtggt gatctatggc aaggatcgcg
     3721 gcgtcgtgcg cgaccgcgcc gaccagctgg ccaagcgcct attcgcccgg cccgacgacc
     3781 tttttgacac cgcccagctg accgagagcg atgtcgagtc
//
LOCUS       DAOMEO010000172         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2792_length_3818_cov_2.462397, whole genome shotgun sequence.
ACCESSION   DAOMEO010000172 DAOMEO010000000
VERSION     DAOMEO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2792_length_3818_cov_2.462397"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..582
                     /locus_tag="PLH31_05165"
     CDS             214..582
                     /locus_tag="PLH31_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQR88651.1"
                     /translation="MRISTAESQVMKALWARSPLSADEIIAALPADQDWADATVKTLL
                     NRLLKKDAIAAERDGRRFLYRPLVAQGDYVHAESQGLLDRLFDGRLAPLVAHFSQREK
                     LSDQDVADLRRLLESLEDGK"
     gene            572..2044
                     /locus_tag="PLH31_05170"
     CDS             572..2044
                     /locus_tag="PLH31_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQR88652.1"
                     /translation="MASDILALLLRANLAIAAAVLVVLIVRRPVRSLFGARAAYGLWL
                     IAPLAVFAVLLPPRTITVQAPAAPTPIATPAVATTATAASATVKPEAAPQQGIQPQRI
                     GDTLVIIWALVALLAIGVQAERQRRFLKSLGRLRGEKDLFHAEHAGVGPAVIGALSPR
                     VVLPADFALRFTPEEQALILAHERNHLSTGDAQINALTIAVQCAFWFNPLVHIGANRL
                     RVDQEIACDAAVLIRYPVARRTYGEAMLKTQLAPHAPPLGCHWPASANKQLKERFVML
                     KQHRQGRARRMTGAAAVAALAMGAGFAAWAAQPVRTVQQTPQQAASAIARHLGQPLFE
                     AVREGDVEGVRDLLAAGANPDARIAGDGTPLIEAARQGRLEIVEMLLEKGANPDLVSR
                     GDGNPLIMAAGWGRLDIVKALVERGASVESFVPGDETPLINAAQSGSLETVRYLVEKG
                     ADVNRSVVANPGESRSPLGQATKRGHLAVIVYLRAKGARA"
     gene            complement(2075..2221)
                     /locus_tag="PLH31_05175"
     CDS             complement(2075..2221)
                     /locus_tag="PLH31_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88653.1"
                     /translation="MAILIGPPASSVARVMPAMPPPTMIRSASIRLASGKVRASMKAI
                     VFPV"
     gene            2166..2453
                     /locus_tag="PLH31_05180"
                     /pseudo
     CDS             2166..2453
                     /locus_tag="PLH31_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
     gene            2513..>2784
                     /locus_tag="PLH31_05185"
     CDS             2513..>2784
                     /locus_tag="PLH31_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQR88654.1"
                     /translation="MLQDPARRPLDLDPRDGFFPAPRYFSKISGGADKAAFDAFRTDF
                     AEAPNITVYLYANVTQVRLDARNDKVEGLDLACLSGKRHTAKAKTYV"
     assembly_gap    2785..2794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2795..3640
                     /locus_tag="PLH31_05190"
     CDS             <2795..3640
                     /locus_tag="PLH31_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HQR88655.1"
                     /translation="VLATGGIENVRLMLASNKVRPEGVGNHHDLVGRFFQGHVTYAMD
                     GDAETEGSAINVARAESMALYVPPGRAQPHCVIGSSLSGQTRMKTGNFTLTLYNGGEP
                     GSGTPTQAETRVIRQTATRVDGGKDEGQLLGCFAMTEQFPNPESRITLDMADVDPLGM
                     PRIKLDWVYSRADWDSFERTAAAVGDALGASGQGRLCWPVERKDYVAICNASRHHMGT
                     TRMHADPRQGVVDADCRVHDIANLYVAGSSVFPTSGIANPTLTLLALAIRLADHLKLE
                     MGAAR"
     gene            3637..>3818
                     /locus_tag="PLH31_05195"
     CDS             3637..>3818
                     /locus_tag="PLH31_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88656.1"
                     /translation="MTPPVHQGLARRDLLTTLLALTAGAALPTLVRAAEPAGAFADLD
                     RDAVERIGKAWLEQNPG"
BASE COUNT          654 a         1374 c         1178 g          602 t
ORIGIN      
        1 gcgcgtcggg cggccgcacg gcgtcgcctg acagagggcc gccgcaagta ggtccgcgcc
       61 agagcggcgc ccaaaatcgc cgagccgtcg ccaaagccgt agcccgcacc aggcttcctg
      121 gcaaaaatcg gatattacat ttcgtttacg tccgtaatcg ttgattgacg agatcgatta
      181 cgaatgtaat cgatggcgct caaggagagc tccatgcgga tcagcacggc cgaaagtcag
      241 gtaatgaaag cgctgtgggc caggagcccg ctcagcgcag acgagattat cgccgccctg
      301 cccgctgatc aggactgggc agacgcgacc gttaagaccc tgctcaaccg gctgctgaaa
      361 aaggacgcga tcgctgcgga gcgcgacggc cgtcgctttc tctatcgtcc gctggtcgcc
      421 cagggcgact atgtgcacgc cgagagccag ggcttgctgg accgactgtt cgatggccgc
      481 ctggcgccgc tggtggctca tttctcacag cgcgaaaaac tgagcgacca ggacgtggcc
      541 gatctgcgcc gtctgctgga gagcctcgaa gatggcaagt gacatccttg cgctgctgct
      601 gcgcgccaac ctggctatcg ccgccgctgt cctggtggtg ctgatcgtgc gccgtccggt
      661 gcgctcgctg ttcggggccc gcgcggccta tggcctctgg ctgatcgcgc ccctggcggt
      721 gttcgcggtt ttgctgccgc cgagaaccat aacggtccaa gctccggctg cgccaacgcc
      781 catcgctact cccgccgtcg cgacgacggc caccgccgct tccgcaacgg tcaaaccgga
      841 agccgcccct cagcagggca tccagcccca gcgcatcggc gacaccctcg tcatcatctg
      901 ggccctggtc gccctgctgg ctatcggcgt ccaggccgag cgccagcgcc ggttcctgaa
      961 gtcgcttgga cgcctgcgcg gcgaaaagga cctgttccat gccgagcacg ccggggtcgg
     1021 tcccgccgtg atcggcgccc tgtcgccgcg ggttgtgttg cccgccgact tcgccctgcg
     1081 cttcacaccc gaagagcagg ctctgatcct ggcccacgag cgcaatcacc tgtcgaccgg
     1141 cgacgcccag atcaacgccc tgaccatcgc ggtccagtgc gcgttctggt tcaaccctct
     1201 cgtccatatt ggagccaatc gcctgcgggt cgatcaggag atcgcatgcg acgccgctgt
     1261 cctgatccgc tacccggtcg cccgccgcac ctatggcgag gccatgctga agacccaact
     1321 ggcgccgcac gcgccgccgc ttggctgcca ttggccagcc tcggccaaca agcaactcaa
     1381 ggagaggttc gtcatgctca aacaacaccg ccagggccgg gcccgacgca tgacgggcgc
     1441 cgccgccgtc gccgcactgg ccatgggcgc gggcttcgcc gcctgggctg cccagcccgt
     1501 ccgcaccgtc cagcaaacac cgcaacaggc ggccagcgcc atcgctcgtc accttggaca
     1561 accgcttttc gaggctgtcc gcgaagggga cgtcgaaggc gtgcgcgatc ttctggccgc
     1621 cggggccaat cctgacgccc ggatcgccgg ggatggcacg cctctgatcg aggccgctcg
     1681 ccagggccga cttgagatcg tcgagatgct gctggagaag ggcgccaatc ctgacctcgt
     1741 ctcccgcggc gacggcaacc cgctgatcat ggccgccgga tggggtcgcc tcgacatcgt
     1801 caaggccctg gtcgaacgtg gagcctcggt ggagtccttc gtacccggcg acgagacgcc
     1861 cctgatcaac gccgcccagt cgggcagcct ggagaccgtg cgctacctcg tcgagaaggg
     1921 cgccgacgtg aaccgctccg tcgtcgccaa tccgggcgaa agccgctcgc cgctcggcca
     1981 ggccaccaaa cgcggccacc tcgccgtcat cgtctatctc agggccaagg gcgcgcgcgc
     2041 ctaagcggcg cgccctccac ccctctccaa acaatcagac aggaaacacg atggctttca
     2101 tcgacgcccg aaccttgcct gacgccagcc ggatcgaggc cgatctgatc atcgtcggcg
     2161 gcggcatggc cggcatcacc ctggccacgg aactggccgg ggggccgatc aagatcgcca
     2221 ttctcgaagc cggcggccgc gagatcgaca tggaaaacca ggcgctctac gtaggttcag
     2281 gtgtgatgcg cggcccggac aatgcggacc gcccgttcaa cgactatcca gtgcagtcgc
     2341 gtatccgcgc cctcggcggc tcggctcacg tctggggtgg caaatgcgcc ccgctcgacc
     2401 cggccgactt cacccagcga gactgggcgc cctacagcgg ctggcccatg agccgccagc
     2461 agctacaacc gttctatgac cgggcctgcg atctatcggc cccgacacgc cggtgctcca
     2521 ggacccggcc cgcaggccgc tggatctgga tccccgggac ggcttcttcc ccgccccgcg
     2581 ctatttctcg aagatctcag gcggcgccga caaggccgcc tttgacgcct ttcgcaccga
     2641 cttcgccgag gcgcccaaca tcacggtcta tctctacgcc aacgtcaccc aggtgcggct
     2701 agacgccagg aacgacaagg tcgaaggtct cgacttggcc tgcctgagcg gcaagcgcca
     2761 tacggccaag gccaagacct atgtnnnnnn nnnngtgctg gcgaccggcg gcatcgagaa
     2821 cgtccgcctg atgctggcgt caaacaaggt gcgtcccgaa ggcgttggca accaccacga
     2881 tctggtcggc cggttctttc agggccacgt cacctacgcc atggacggcg atgccgagac
     2941 cgaaggctcg gcgatcaatg tggcgcgcgc cgagtcgatg gcgctctatg tgccgcccgg
     3001 ccgcgcccag ccccactgcg tgatcggcag cagcctgtct ggccagacac gaatgaagac
     3061 cggcaacttc accctgacgc tctacaatgg cggcgagcca ggctcgggca cacccaccca
     3121 ggccgagacc agggtcattc gccagacggc gacgcgcgtc gacggcggca aggacgaagg
     3181 ccaattgctg ggctgcttcg ccatgaccga gcaatttccc aatcccgaaa gccgcatcac
     3241 gctggatatg gccgatgttg accctctggg catgccgcgc atcaagctgg actgggtcta
     3301 ttcacgggct gactgggaca gcttcgagcg caccgccgcg gccgtcggcg acgccctggg
     3361 cgcatcgggc cagggtcggc tttgctggcc ggtcgagcgc aaggactatg tggcgatctg
     3421 caacgcctcg cgtcatcaca tgggcacgac ccgcatgcac gccgacccca gacagggcgt
     3481 ggtcgacgcc gattgccgcg tccacgacat cgccaacctc tatgtcgccg gcagttcggt
     3541 attcccgacc tccggcatcg ccaatccaac cctgaccctt ctggccctgg cgatccgtct
     3601 ggccgaccac ttgaagcttg agatgggagc cgcccgatga ccccgccagt ccaccagggc
     3661 cttgcccgcc gcgacctcct gaccaccttg ttggccctga ccgccggtgc cgccttgcca
     3721 accctggtcc gcgccgccga gccagcgggc gccttcgccg atctggaccg cgacgccgtt
     3781 gaacgcatcg gtaaggcctg gctggaacag aaccccgg
//
LOCUS       DAOMEO010000173         3806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2801_length_3806_cov_2.154625, whole genome shotgun sequence.
ACCESSION   DAOMEO010000173 DAOMEO010000000
VERSION     DAOMEO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2801_length_3806_cov_2.154625"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLH31_05200"
     CDS             <1..341
                     /locus_tag="PLH31_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR88657.1"
                     /translation="GGAQGVLVTAVSPIAFSQALGMVRRGGTVSLNGLPPGDFPLSIF
                     DTVLRGITVRGSIVGTRLDLQEALAFAADGRVRATVHAEALANINDVFGRMARGEIEG
                     RVVLDFEDRS"
     gene            338..784
                     /locus_tag="PLH31_05205"
     CDS             338..784
                     /locus_tag="PLH31_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR88658.1"
                     /translation="MSGVVRICAGLLAGLALGLTAAQAVADSKPAPAPAPAPAPAPAP
                     AQVLGEQGIAQRNCGTCHAVAPGSDLSPLADAPPFATLYRRYPPGGLDQILTEGMLAP
                     SRPPEEGSPNRHPRMPMVQLDDDEVAQLRAYLRRLDPRPQPREGQR"
     gene            919..1062
                     /locus_tag="PLH31_05210"
     CDS             919..1062
                     /locus_tag="PLH31_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88659.1"
                     /translation="MTALQLAFIILNVAAFVAFVLTLGAVSVQAAAGEGPTMRRSHRR
                     AAR"
     gene            1232..1765
                     /locus_tag="PLH31_05215"
     CDS             1232..1765
                     /locus_tag="PLH31_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88660.1"
                     /translation="MNFPRGRAPRPPAAPRAGRSGFDALWIFPAVALAAPLMVGVSAS
                     MLQACLMLGAATGLFVVGSAGTFWALLVWGAATGGLGAFLAFRDAPNGQSLTVVVLAV
                     AAAQGLALLGVARPASTAGWSARGRVLLSLVGPLALIILALRGPPDWRSLGAAVAVAL
                     DLAALPLATEARRRAGS"
     gene            1839..2492
                     /locus_tag="PLH31_05220"
     CDS             1839..2492
                     /locus_tag="PLH31_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HQR88661.1"
                     /translation="MIAYDQRTRFLAAGLSALAGFVDALGFMHLGGFFVSFMSGNSTR
                     FGVGLANTSTTAGIAGGLIITFVLGVMAGSWLGLGSGSRRRFAVLALVSGLLAMGAVF
                     AGLGATWLAVGAMTLAMGAENAVFDRDGEVDVGLTYMTGALVKLGQRLVLALRGGPAW
                     RWATSLLLWGGLVAGALLGALAHKHLGLQALWLAAVASALLAVIAARLDTDRRASAT"
     gene            complement(2489..2941)
                     /locus_tag="PLH31_05225"
     CDS             complement(2489..2941)
                     /locus_tag="PLH31_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR88662.1"
                     /translation="MTRNLPISDVIAPETRGRPKARARSRWLLASLVASASAALAGCA
                     VLQPLNAGPDLPLVETGRALALSECSTCHAVGAGGGAPTTEAPSFISIAERYRNYRLD
                     WELESISQVGHYRMRPKMLTSAEISALAAYIRTLDSARPAEVATPPLR"
     gene            complement(3056..3391)
                     /locus_tag="PLH31_05230"
     CDS             complement(3056..3391)
                     /locus_tag="PLH31_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR88663.1"
                     /translation="MISSVQLAPIDATQDLAASAHAAARLLKLLASEQRLLILCRLID
                     GEASVGDLSIHVGLAQSATSQHLAKMRAEGLVATRREAQTIFYSLADPAAVRVLETLC
                     DIYRGAQAR"
     gene            complement(3437..>3806)
                     /locus_tag="PLH31_05235"
     CDS             complement(3437..>3806)
                     /locus_tag="PLH31_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88664.1"
                     /translation="GIVIGHILFKAPFSATSMIGFIALAGIIVRNSILLVDFIRHSQT
                     GERPLREVLLEAGAIRFKPIVLTALAAMIGAAVILFDPIFQGLAISLLFGLASSTVLT
                     VLVIPAIYVVLRDDAPAAKV"
BASE COUNT          562 a         1238 c         1311 g          695 t
ORIGIN      
        1 tcggcggcgc ccagggcgtt ctggtcacgg ccgtgtcgcc aatcgccttt tcacaggccc
       61 tgggtatggt ccggcgcggc ggtacggtgt cgctgaacgg cctgccgccg ggtgacttcc
      121 ctctatcgat cttcgatacg gtgctgagag ggatcacggt gcgcggctct atcgtcggaa
      181 cgcgtctgga ccttcaggaa gccctggcct ttgcagccga cggccgcgta cgggcgaccg
      241 tccatgccga agcgctggcc aatatcaacg acgtctttgg ccgtatggcg cggggcgaga
      301 tcgaaggtcg ggtcgttctc gatttcgagg atcggtcatg agcggcgtcg tgaggatctg
      361 cgcaggactt ctcgccggtc tggccttggg cttgacggcg gcccaggccg tcgccgactc
      421 caagcctgcg cctgcgcctg cgcctgcgcc tgcgcctgcg cctgcgcctg cgcaggtgct
      481 tggcgagcag gggatcgccc agcgcaattg cggaacctgc catgccgtgg cccctggctc
      541 cgacctaagc cccctggccg acgccccgcc tttcgccacc ctctatcggc gttatccgcc
      601 gggcggcctc gaccagatcc tgaccgaggg catgctcgcg cccagccgtc cgccagaaga
      661 gggctcgcca aaccgtcacc cgcgcatgcc gatggtccag ctggacgatg atgaggtcgc
      721 tcagctgagg gcctatctgc gacgcctcga cccgcggcct caacctcgcg aggggcagcg
      781 atagaccaag ccccggagat cccgccccgt gacgccccta cagccttcgc ggcggcggac
      841 tttacccgcg attgagcgag atcaagacca cggtcgcggc ttcgagagat cattttctaa
      901 gaacaggagg ttcgcgtcat gaccgctctc caactggcct tcatcattct gaacgttgct
      961 gcgtttgtcg cctttgttct gaccctgggc gccgtatccg tacaggccgc tgcgggcgag
     1021 gggccgacca tgcgcaggtc tcaccggagg gctgcgcgat gaaccgccgt ccccctgtca
     1081 aacttagaac cgttctgatc gttgcgaccg tgcttctggg cgtcgcgtct gttgccggct
     1141 gccagttgcg gcaattccgt cagccctccc acgagcctgc cgtacagccg ttgaacgttc
     1201 gctaggtgag gcgcccaggg aggtcttgcc gatgaacttt ccccgcggac gggcccctcg
     1261 acccccggcg gcgccccgag ccgggcggtc gggcttcgat gcgctctgga tcttccccgc
     1321 ggtggccctg gccgccccgc tgatggtcgg tgtctcggcc tcgatgctgc aggcctgcct
     1381 catgctgggc gcagccaccg gtctctttgt ggtcggatca gccgggacgt tctgggccct
     1441 gctggtctgg ggcgcggcga ccggcggact gggggccttt ttggccttcc gcgatgcgcc
     1501 gaacggccag agcctcaccg tggtcgtgtt ggcggtggcg gcggctcaag gactcgcgct
     1561 gctcggtgtc gcccgtccgg cgtcgacggc gggatggagc gcgcgggggc gggtgcttct
     1621 ttcgttggtc gggccccttg ccctgatcat ccttgctctc agggggccgc ccgactggcg
     1681 cagcctcggc gcagcagtgg cggtggctct ggatctggcg gcgctgccgc tggccacaga
     1741 ggcccggcgg cgcgcagggt catgagcgcg ttctgcattg aagagtcgca ccgggcgcca
     1801 ggagcaggcc tggacatgcc gtcttttgag gtggttgtat gatcgcctat gaccagcgca
     1861 cccggttcct ggcggccgga ctttcagcac tggctggctt cgtcgacgct ttgggattca
     1921 tgcacctggg cggctttttc gtctcgttca tgagtgggaa ctcgacgcgc ttcggggtcg
     1981 gcctggccaa cacctcgacg accgccggca ttgcaggcgg cttgatcatc accttcgtgc
     2041 tcggcgtcat ggccggatcc tggcttgggc tcggcagtgg aagccggcga cgctttgctg
     2101 tgctggcgct ggtgagcggt ctgctggcga tgggcgcggt ctttgccgga ctgggcgcca
     2161 catggctggc cgtcggcgcg atgaccctgg ccatgggcgc cgagaatgcg gtgtttgacc
     2221 gtgacggcga ggtcgatgtc ggcctgacct atatgacggg cgctctggtc aagctggggc
     2281 aacgcctggt gctggccctg agaggcgggc ccgcctggcg atgggcgacg tccctgctgc
     2341 tctggggagg cctggttgca ggcgctctgc ttggggccct ggcgcacaag catctgggcc
     2401 ttcaagctct ttggcttgca gccgttgcgt cggctctact ggcggtgatc gccgcgcgac
     2461 tggacaccga tcgcagggcg tccgcgacct aacggagcgg tggcgtggcg acctcggcgg
     2521 gcctggcgct gtcgagcgtt cggatatagg ccgcgagcgc gctgatttca gcagaggtga
     2581 gcatcttggg ccgcatccga tagtgaccca cctgcgagat gctctccagt tcccagtcca
     2641 gccggtagtt gcgatagcgc tcggcgatgc tgatgaaaga gggggcctcg gtggtcggcg
     2701 cgccaccgcc ggcgccgacc gcgtggcagg tggaacattc actgagcgcc agggcccggc
     2761 cagtctcgac caggggaaga tcaggccccg cattcagcgg ctgaagcacc gcgcagccgg
     2821 ccaaggcggc tgatgccgag gctacaaggc tcgcaaggag ccaccttgac ctggctcgcg
     2881 ccttgggccg cccacgcgtt tccggggcga tgacgtcgga aatcggaagg ttgcgggtca
     2941 tgggacgctc ctgaatcctg ggcctagcct gatcggcctg gatcgccgcc atcttgaccg
     3001 agatcaaacc cgtacgtctt gactgaagcc cgtcgtattc gccgtcgatg gcggatcagc
     3061 gcgcctgagc gccccggtat atgtcgcaca gggtttcgag cacccgcacg gcagccgggt
     3121 cggccagact gtagaagatg gtctgggcct cacgccgcgt agccaccagc ccctcggcgc
     3181 gcatcttggc caggtgctgc gaggtcgctg attgagcaag gccgacatgg atcgacagat
     3241 cgcccaccga ggcctcgcca tcgatcagac ggcagaggat tagcagccgt tgctcgctgg
     3301 ccagcagctt cagcagccgc gccgcagcat gggcgctggc ggcgagatcc tgggtggcat
     3361 cgattggggc aagctgtacg gaactgatca tctgcctaat gtagaagata tgcatacagg
     3421 ttcaagttaa gcggcctcag accttggcgg ccggggcgtc gtcgcgcagc accacataga
     3481 tggccggaat gaccagcacg gtcagcacgg tcgacgaggc caggccaaac agcagcgaga
     3541 tcgccagacc ctggaagatc gggtcgaaca ggatcaccgc cgcgccgatc atcgccgcca
     3601 gggcggtcag cacgatcggc ttgaagcgga tcgccccggc ctccagcagc acctcgcgca
     3661 gcggccgctc accggtctgg ctatggcgga tgaagtccac cagcaggatc gagttgcgca
     3721 cgatgatccc ggccagggcg atgaagccga tcatcgaggt ggcgctgaac ggcgccttga
     3781 acaggatgtg tccgatgacg atgccg
//
LOCUS       DAOMEO010000174         3804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2804_length_3804_cov_2.525207, whole genome shotgun sequence.
ACCESSION   DAOMEO010000174 DAOMEO010000000
VERSION     DAOMEO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_3804_cov_2.525207"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>248
                     /locus_tag="PLH31_05240"
     CDS             <1..>248
                     /locus_tag="PLH31_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HQR88665.1"
                     /translation="LARRGAAPLFDRVEVDNGDEAALRQWQAHLGVLTGHTDAPDWSR
                     PAYGRWTLAQRRLLNPGSPGGEAWHIRLEPLDGVLDWS"
     assembly_gap    249..258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <259..982
                     /locus_tag="PLH31_05245"
     CDS             <259..982
                     /locus_tag="PLH31_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HQR88666.1"
                     /translation="ISRLGLPASMAEALATRRLPHDVEPLIGLTGEAVLERLTALPHR
                     EYSIASLPADGGIELIVRLMPRADGRPGLGSGWLCHYAPEGAVIDARVRVNRSFHAPD
                     GDAPMILIGAGTGLAGLRAHLKARVAEGRGRNWLLFGERTSAHDAFHRDELSAWVSSG
                     VLERLDLAFSRDQAERVYVQHRLRDAADAVRAWVTDGATIMVCGSLEGMSREVHATLA
                     DILGAEGLETLTDQGRYRRDVY"
     gene            complement(1145..1675)
                     /locus_tag="PLH31_05250"
     CDS             complement(1145..1675)
                     /locus_tag="PLH31_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQR88667.1"
                     /translation="MTPFGALILTGGGSRRMGRDKAALDWDGARAVDRVADVARAVGA
                     RDVLTVGAELGLPWVPDPEPGAGPVGGVLAGVAALRALGLDRALILAVDAPTLTVEDL
                     APLLAAQAPGAAYEGLPLPMALTFAALPEDAVAGWPLRRLVERTGLLTLALPAEAEAR
                     LRGANTPEERARLLRP"
     gene            1873..>2316
                     /locus_tag="PLH31_05255"
     CDS             1873..>2316
                     /locus_tag="PLH31_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR88668.1"
                     /translation="MREPTERQRRRTTQSATIRDVAARAGVSPMTVSRVINRETTVKE
                     ETRVLVEQAITDLNYAPNPAARSLAGSAPFRIGLLYDNPSTGYLSEFLVGALDESSRT
                     GAQVVIEKCAEPELAGATLTRLLRTGVDGLILPPPLCESPQVLAEV"
     assembly_gap    2317..2326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2327..2911
                     /locus_tag="PLH31_05260"
     CDS             <2327..2911
                     /locus_tag="PLH31_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR88669.1"
                     /translation="VATGTASADMATIRIDNEAAAYELTQHLLGLGHKRFGFIKGHPN
                     QTVSQQRLDGFMTALKAAGIPQENVRVEQGYFTYRSGLEAAERLLASDERPTAIFACN
                     DDMAAAAAGLAHRLGLDVPGDVSIVGFDDTSIAANIWPALTTVHQPIAAMARAAVDLV
                     LEEIRRKRDGSGEPRQLMHPHTLIVRDSSGPAPK"
     gene            3087..>3804
                     /locus_tag="PLH31_05265"
     CDS             3087..>3804
                     /locus_tag="PLH31_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HQR88670.1"
                     /translation="MTQFLPEDWQDATLLGRVDFGDGPTPILVRGGRIEDMSRIAPTL
                     ADLMNAYGPGAELPRGEDKGPLEALDVRPVWADASGEAAAKLLAPVDLQCLKAAGVTF
                     AVSTLERVIEERARGDAAEALKIREQLADSMGGDLRSVDPGSEGAERLKQTLIQDGLW
                     SQYLEVAIGPDAEIFTKAQPMSAVGIGAEIGIHPHSDWNNPEPEIVLAVNSRGEVKGV
                     TLGNDVNLRDFEGRSALLLGK"
BASE COUNT          592 a         1407 c         1278 g          507 t
ORIGIN      
        1 ggctggcccg gcgcggcgcc gcgcccctgt tcgaccgcgt cgaggtcgac aacggcgacg
       61 aggccgccct gcgccagtgg caggcccatc tgggcgtcct gaccggccac accgacgcgc
      121 ccgactggag ccgtccggcc tatggccggt ggacactagc ccagcgccgg ctgctcaacc
      181 ccggcagtcc gggcggcgag gcctggcaca tccggctgga gccgctggac ggcgtcctgg
      241 actggtcgnn nnnnnnnnga tctcgcgcct gggcctgccg gcgtcgatgg ccgaggccct
      301 cgcgacccgc cgcctgcccc acgacgtcga gcccctgatc ggcctgacag gcgaggcggt
      361 gctggagcgc ctgaccgccc tgccgcaccg ggaatattcc atcgcctccc tgccggccga
      421 tggcgggatc gagctgatcg tccgcctgat gccccgcgcc gacggcaggc cgggcctcgg
      481 ctcgggctgg ctgtgccatt acgcccccga aggcgcggtg atcgacgccc gggtgcgggt
      541 gaaccgcagc ttccacgctc ctgacggtga cgccccgatg atcctgatcg gcgcaggcac
      601 aggcctggct ggcctgcgcg cccacctcaa ggcccgcgtc gccgagggcc ggggccgaaa
      661 ctggctgctg tttggcgagc gcacctcggc ccacgacgcc ttccaccgtg acgaactgtc
      721 ggcctgggtt tcaagcggcg tgctggagcg cctggatctg gccttctccc gcgatcaggc
      781 cgagcgggtc tatgtgcagc accgcctgcg cgacgcggcc gatgcggtgc gggcctgggt
      841 gacggacggc gcgacgatca tggtgtgcgg ctcgctggaa ggcatgtcgc gcgaggtcca
      901 cgcgacgctc gcggacatcc tgggcgccga ggggctggag acgctcaccg atcaggggcg
      961 gtatcggcgg gacgtgtact aggtcggccg acgccggacg ccccctccgt cacgtcgcgt
     1021 atccgcgccg cgccacctcc cccgcaagcg gggcaggaga agagcgattg tcaccctcct
     1081 gccccgtagc gaagcgtgcg ggggaggtgg cgcgctgcga atacgcagcg tgacggaggg
     1141 ggcgctacgg cctcagcaac cgcgcccgct cctccggcgt attggccccc cgcaaccgcg
     1201 cctcggcctc ggccggcagg gccaaggtca gcaaacccgt acgctcgacc agccgccgca
     1261 gcggccatcc cgcgaccgca tcttcgggca aggccgcgaa cgtcagagcc atcggcagcg
     1321 gcaggccctc ataggccgcc cccggagcct gcgccgccag caagggcgcg aggtcctcga
     1381 ccgtcagggt tggcgcatca accgccagga tcagggcccg gtccagcccc agcgcccgca
     1441 gcgccgcgac acccgccagt acgccgccga ccggccccgc gccgggctcg gggtcgggaa
     1501 cccagggcag gcccagctcc gcgccgacgg tcaggacgtc cctcgctccg accgcccgcg
     1561 ccacgtcggc cacgcggtcg acggcccggg cgccatccca gtccagcgcg gccttgtcgc
     1621 ggcccatgcg gcgcgaaccg ccgccggtaa ggatcagagc gccgaagggt gtcatggccg
     1681 ttccagcaaa tcctctcccc agtggggaag gtgtcgccgc aggcgacgga gggggaaata
     1741 accgccaatt cggtgcggtc ttcccccacc ggcctggcgg ccgcctcccc cgctaggggg
     1801 aggatctttc cccgtcttcc actctaacgg cgaaccggtc tagtgtccgc ccccagaggg
     1861 acgggacaac ccatgagaga accgacggag cgccagaggc ggcgcacaac ccagagcgcg
     1921 accattcgcg atgtcgcggc gcgcgccggc gtgtcgccca tgaccgtctc gcgggtcatc
     1981 aatcgcgaga ccacggtcaa ggaagagacc cgggttctgg tcgagcaggc catcaccgac
     2041 ctcaactacg cgcccaatcc cgccgcgcgc agcctggccg gctcggcgcc gttccgcatc
     2101 ggcctgctct atgacaaccc ctcgaccggc tatctgtcgg agttcctggt cggggcgctg
     2161 gacgaaagca gccgcaccgg cgcccaggtg gtgatcgaga aatgcgccga gcccgaactg
     2221 gccggcgcta ccctgacccg cctgctgcgc accggcgtgg atggcctgat cctgccgccg
     2281 ccgctgtgcg aatcgcctca ggtcctggcc gaggtcnnnn nnnnnngtcg ccaccggcac
     2341 ggccagcgcc gacatggcca ccatccgcat cgacaacgag gccgccgcct atgagctgac
     2401 ccagcacctg ctgggcctgg gccacaagcg ttttggcttc atcaagggcc atcccaacca
     2461 gacggtcagc cagcagcgcc tggacggctt catgaccgcc ctgaaggccg ccgggatccc
     2521 gcaggagaac gtccgcgtcg agcagggcta tttcacctat cgctcgggtc tggaggcggc
     2581 cgagcggctg ctggccagcg acgagcggcc caccgccatc ttcgcgtgca acgacgacat
     2641 ggccgccgcc gccgccggcc tggcccatcg cctggggctg gacgtgccgg gggacgtctc
     2701 catcgtcggc tttgacgaca cctcgatcgc cgccaatatc tggccggccc tgaccacggt
     2761 gcaccagcct atcgccgcca tggcccgcgc ggccgtggac ctggtgctgg aagagatccg
     2821 ccgcaagcgc gacggctcgg gcgagccccg gcaactgatg catccccaca ccctgatcgt
     2881 gcgggattca tcgggaccag cccccaaata attcaccgcc gcgccgtcgc cgtcacccgg
     2941 ggtgagccca ttcctcccac aggattggtc ttacagagct tgccgcctac acatgttagc
     3001 gctaacaagt gcgcgagcat caacgagcgc ggctcccagg gctttggtgg gtccagacgc
     3061 tcgatagggg agacgacgcc gtgggcgtga cgcaattcct tccggaagac tggcaagacg
     3121 caaccctgct gggccgcgtg gacttcggcg acggcccgac gccgattctg gtgcgcggcg
     3181 gccggatcga ggacatgtcc aggatcgccc ccaccctggc cgacctgatg aacgcctatg
     3241 gccccggcgc ggagcttccg cgcggcgaag acaagggccc gctggaagcc ctggatgtcc
     3301 gcccggtctg ggccgacgcc tcgggcgagg ccgccgccaa gctgctggcc cccgtcgacc
     3361 tgcaatgcct gaaggccgct ggcgtcacct tcgccgtctc cacccttgag cgggtgatcg
     3421 aggagcgcgc ccggggcgac gccgccgagg ccctgaagat ccgcgaacaa ctggccgaca
     3481 gcatgggcgg cgacctgcgc agcgtcgatc ccggctcgga gggcgccgag cgcctgaagc
     3541 agaccctgat ccaggacggc ctgtggtcgc agtatctgga agtggccatc ggccccgacg
     3601 ccgagatctt caccaaggca caacccatga gtgccgtagg catcggcgca gagatcggca
     3661 tccacccgca cagcgactgg aacaaccccg aacccgagat cgtgctggcc gtcaacagcc
     3721 gcggcgaggt caagggcgtc acgctgggca atgatgtcaa cctgcgcgat ttcgaaggcc
     3781 gcagcgccct gttgctgggc aaat
//
LOCUS       DAOMEO010000175         3801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2807_length_3801_cov_2.556327, whole genome shotgun sequence.
ACCESSION   DAOMEO010000175 DAOMEO010000000
VERSION     DAOMEO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2807_length_3801_cov_2.556327"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLH31_05270"
     CDS             complement(<1..694)
                     /locus_tag="PLH31_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 Nuclease"
                     /protein_id="HQR88671.1"
                     /translation="MSRLSSSLTVLALAAALAAPANSALAWGASGHRLIGVLGAQSLP
                     LDVPAFVRTPAAIATIGEYARELDRSKGSGKIHDHDRDSAHFLDVDDEGRMFGGPMFT
                     VATLPPTRADYETALRAVGMDSWKAGYLPYAMIDGYQQLVKDFTYWRILVAAEKSATD
                     PVRKAYYAADLKRREDLLLRDLGVWAHYVGDASQPLHLSVHYNGWGDYPNPNGYTNSR
                     AIHSSFEGPLAKA"
     gene            complement(710..1297)
                     /locus_tag="PLH31_05275"
     CDS             complement(710..1297)
                     /locus_tag="PLH31_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR88672.1"
                     /translation="MTMRIYGDTKSGNCLKVKWTAQQLALPFDWVEVDILQAQSRTPE
                     FLAMNPAGQVPAVVLGDGRTLAQSNAIMLHLAEGSALIPGDPFERAKMYEWLFWEQYS
                     HEPYVAVARFHVLYLGKPVGELDAKLVERGGAALARLETQLKQTPYLVGERLTLADIA
                     LVAYTRVAHEGGFDLSLYPAVKAWVARVEVALDIH"
     gene            1384..1881
                     /locus_tag="PLH31_05280"
     CDS             1384..1881
                     /locus_tag="PLH31_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HQR88673.1"
                     /translation="MRRTLAGILAFLAMSTALPVFAADSDSAARPDPLGDLITGALTG
                     TIPGTLEYKMKATLYHAGAKGIRALDSLGCKVVAMRTVAVDKTVIPRRTVLFIKETVG
                     LPMPDGRTHDGYWYASDVGGAIKGLKLDLFSGSGRSSMAPLMPLNLTHLSVSKVGEFK
                     GCPPE"
     gene            1964..2362
                     /locus_tag="PLH31_05285"
     CDS             1964..2362
                     /locus_tag="PLH31_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HQR88674.1"
                     /translation="MILPKGATVAVVDGEKLVMFKNTGEHEPQLTVLPAEAIDGAGSG
                     SKGHQSSSANPDESTRAEDGYAAGVAAMLNERALSGAFDHLLVIAAPKTLGELRKHWG
                     KPLQAKLVGEIAKDLTGQSADQIVTAIDKA"
     gene            complement(2404..>3801)
                     /locus_tag="PLH31_05290"
     CDS             complement(2404..>3801)
                     /locus_tag="PLH31_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88675.1"
                     /translation="NPALTAMAGPGAKAGVVFGELIEPVTRAEAETRLAGGKAGPFEV
                     RLSRDPTRIAHLYLYRAEGRLVAYLIDVSEQKQVELQLAQAQKMQAIGQLAGGVAHDF
                     NNLLTAIQLRLDELLHRHPVGDPSYEGLNEIRQTGVRAADLVRKLLAFSRKQTVQREV
                     LDLGELISEFEVLLRRLLREDVKLITDYGRDLPQVRADKSQLETAVMNLAVNARDAVR
                     AAKGGGIVRIRTARLTQEEAVALGFPGADGDSDNGPGIPPDVMGKIFDPFFTTKPVGE
                     GTGLGLATVYGIVKQSDGWIHVHSRPDEGAAFRIFLPVHVPTAAAIAEAAAVAAEPPK
                     ARPPRDLSGAGRILFVEDEDALRGVAARLLRARGYEVLEACDGEEALLIAEANAGTID
                     LLISDVIMPGIDGPTLLKKARGYLGNAPVMFISGYAEAEFSDLLEGETGVTFLPKPID
                     IKTLAERVKQQLQAA"
BASE COUNT          642 a         1268 c         1272 g          619 t
ORIGIN      
        1 ccgccttggc caacgggcct tcgaagctgg agtggatggc gcgcgagttg gtgtagccat
       61 tgggattggg atagtcgccc cagccattgt agtgcaccga cagatgcagc ggctggctgg
      121 cgtcgcctac atagtgggcc cagacgccca gatcgcgcag cagcaggtcc tcgcggcgct
      181 tcaggtcggc ggcgtaatag gctttccgga ccggatcggt cgccgacttt tcggccgcca
      241 ccaggatgcg ccagtaggtg aagtccttca ccagctgctg gtagccgtcg atcatcgcat
      301 agggcagata gccggccttc cagctgtcca tgccgacggc gcgcagggcg gtctcgtagt
      361 cggcgcgggt gggcggcagg gtcgcaaccg tgaacatcgg ccccccgaac atccgccctt
      421 cgtcatcgac atccaggaag tgggccgagt cgcggtcgtg atcgtggatc ttgcccgagc
      481 ccttggagcg atccagttca cgcgcatact cgccgatcgt ggcgatggcg gccggcgtgc
      541 gcacaaaggc cggcacgtcc aagggcaggc tctgggcccc cagcacgccg atcaggcggt
      601 gtcctgacgc gccccatgcc agagcagaat tggcgggcgc ggcgagagcc gccgcgaggg
      661 caagaaccgt gaggctggac gacaggcgcg acatggaagc tccgcgaaac taatgaatgt
      721 ccagggcgac ctcgacccgg gcgacccatg ccttgacggc cggatacagc gacaggtcga
      781 aaccgccctc gtgggcgacc cgggtgtagg ccaccagcgc gatatccgcc agagtaagcc
      841 gctcgcccac cagatagggc gtctgtttca gctgtgtttc caggcgcgcc agggccgccc
      901 cgccgcgttc caccagcttg gcgtccagct cgccgaccgg cttgcccaga tagagcacat
      961 ggaagcgggc gacggcgaca tagggctcgt ggctgtactg ctcccagaac agccactcgt
     1021 acatcttggc gcgttcgaac ggatcgccgg ggatcagcgc cgagccctcg gccaggtgca
     1081 gcatgatggc gttggattgg gccagggtcc ggccatcgcc gaggacgacc gccggcacct
     1141 gaccggccgg gttcatcgcc aggaattccg gcgtgcgaga ctgcgcctgc aggatgtcga
     1201 cttcgaccca gtcgaacggc agggccagtt gctgggccgt ccacttcacc ttcaggcagt
     1261 tgcccgattt cgtgtcgccg tagatgcgca tggtcatcgc tcgcctccga agggttgtca
     1321 cggcgccgtg atcggactat tcagagcccg cccgacagat acgccgataa gaggattccc
     1381 tcgatgcgtc gcacgctcgc cggaattttg gccttcctgg ccatgtccac cgcccttccc
     1441 gtcttcgccg ccgactcgga ctccgccgcg cgtcccgatc cgctgggcga cctgatcaca
     1501 ggcgcgctga ccgggacgat tccgggcacg cttgaataca agatgaaggc gacgctttac
     1561 cacgccggcg ccaagggcat ccgggcgctg gactcgctgg gctgcaaggt cgtggccatg
     1621 cgcaccgtgg cggtcgacaa gaccgtgatc ccgcgccgca ccgtgctctt catcaaggaa
     1681 accgtcggcc tgcccatgcc cgacggccgc acacatgacg gctactggta cgcctccgac
     1741 gtgggaggcg cgatcaaggg cctgaagctg gacctgttca gcggctcggg ccgctcgtca
     1801 atggcgccgc ttatgcccct gaatctgacg catctgtcgg tcagcaaggt cggcgaattc
     1861 aaaggctgcc cgcccgaata gggcgctaga ccgcatctgc cgtctgcggt cgcaacctcc
     1921 accgtcgcca cccgttagcg tgcctcacaa gaggatacgc cgcatgatac ttcccaaggg
     1981 cgccaccgta gccgtcgtcg acggcgaaaa gctggtcatg ttcaagaaca ccggggaaca
     2041 cgagccccag ctgaccgtcc ttccggcgga agccatcgat ggcgccggct caggctcgaa
     2101 gggccatcag tccagttccg ccaacccgga cgagagcacg cgggccgaag atggctacgc
     2161 cgctggcgtc gcggccatgc tgaacgagcg ggctctgtcc ggcgcctttg atcacctgct
     2221 ggtcattgcc gcgcccaaga cgctgggcga gctgcgcaag cactggggaa agccgctcca
     2281 ggccaagctg gtcggcgaga tcgccaagga cctgacgggt cagtcggctg atcagatcgt
     2341 cacggcgatc gacaaggcct gacggcgatg gcggggtcgc aagaccccgc cgctatcgtc
     2401 gtttcaggca gcctgcagct gctgcttgac ccgctcggcc agggtcttga tgtcgatggg
     2461 cttgggcagg aaggtcacgc ccgtctcgcc ttccagcagg tcgctgaact cggcctcggc
     2521 atagccgctg atgaacatca caggcgcatt gcccagatag ccgcgcgcct tcttcagcag
     2581 ggtgggcccg tcgataccgg gcatgatcac gtcgctgatc agcaggtcga tggtgccggc
     2641 attggcctcg gcgatcagca gggcttcctc gccgtcgcag gcttccagga cttcataacc
     2701 ccgggcgcga agcagacgcg cggcgacgcc gcgcagggcg tcctcgtcct cgacgaacag
     2761 gatacgaccg gcgccggaca ggtcgcgggg cggacgggcc ttgggcggct cggcggccac
     2821 ggcggcggcc tcggcgatgg cggcggcggt cggcacgtgg accggcagga aaatgcggaa
     2881 ggcagcccct tcgtccgggc ggctgtggac gtgaatccag ccatcgctct gcttgacgat
     2941 gccatacacc gtggccaggc ccagccccgt gccctcgccc accggcttgg tggtgaagaa
     3001 cggatcgaag atcttgccca tgacgtcggg cggaatgccg ggaccgttgt cgctgtcgcc
     3061 atcggcgccg gggaagccga gcgctacggc ctcttcctgc gtcaggcgcg ccgtgcggat
     3121 acggacgata ccgccgccct tggcggcgcg cacggcgtcg cgggcgttga cggccaggtt
     3181 catgaccgct gtttccagct ggctcttgtc ggcccgcacc tgtggcaggt cgcggccata
     3241 gtcggtgatc agcttgacgt cttcacgcag cagacggcgc agcaggacct cgaattccga
     3301 gatcagttcg ccaaggtcca gcacttcgcg ctgcacggtc tgcttgcgcg agaaggccag
     3361 cagcttgcgc accaggtcgg cggcgcgaac gcccgtctgg cggatctcgt tgaggccttc
     3421 atacgaaggg tcgcccaccg ggtgacggtg cagcagctcg tccagccgca gctggatggc
     3481 ggtcagcagg ttgttgaagt cgtgcgccac gccgccggcc aactggccga tggcctgcat
     3541 cttctgggct tgggccagtt gcagctcgac ctgcttctgc tcggacacat cgatcagata
     3601 ggcgaccaga cgcccctcgg cccggtagag atagagatgg gcgatacggg tggggtcacg
     3661 tgacaggcgc acctcgaaag gaccggcctt gccgccagcc agccgggtct cggcctcggc
     3721 gcgggtcaca ggctcgatca gctcgccaaa aaccacgcca gccttggcgc ccggcccggc
     3781 catggctgtc agggcgggat t
//
LOCUS       DAOMEO010000176         3789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2816_length_3789_cov_2.563203, whole genome shotgun sequence.
ACCESSION   DAOMEO010000176 DAOMEO010000000
VERSION     DAOMEO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_3789_cov_2.563203"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLH31_05295"
     CDS             <1..701
                     /locus_tag="PLH31_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88676.1"
                     /translation="EFGDNDYVGNPSLKPETAWGLDVGYERRLGARGVAGVNVFYRDV
                     TDLIELTSIGTGSAGAPALLLQTQNVGDGSVMGIEFDLSTPLTVFGLDNTGVFFNYSW
                     LDSDIDDTFGSRKFNSQSDYVMNVGFIQDLPTFGGAFGATYRKQGEADGRIIGEEVTT
                     SYGADLEIFLEKRFGEKFTVRLTGSNLLNAKKEEVFNKFNSTADQVSRDFDQYELESE
                     EAGPVFQLIARMAF"
     gene            complement(896..1705)
                     /locus_tag="PLH31_05300"
     CDS             complement(896..1705)
                     /locus_tag="PLH31_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88677.1"
                     /translation="MFDGNLRTIQRVAAKVQRVLVVDANPATAKLLADQLRQLGNVQI
                     YPAPTIEKGYAMARAVDPQLIFVEHASAGVDGLMLTRKIRRSDLVCREAPIIMCTAEA
                     TAEAIFGARDAGIHEFMRKPFTIGDLERRLEAVTLKPRDWVEAVQYVGPDRRRFNSAE
                     YKGPRKRKADAAGTVAARLSQALRIVRSAAGALDSDPMQARRALAAQALELRKVGDAV
                     KEPRLVAAAVALEDCMKSEAPGAEARAALLARIDSLLGFMAADDDGGRGAA"
     gene            complement(1785..2924)
                     /locus_tag="PLH31_05305"
     CDS             complement(1785..2924)
                     /locus_tag="PLH31_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88678.1"
                     /translation="MSARRKLKPVRSNDRVTRSLRALERTASTSRVLNLLAIQADSGH
                     RPEHAQHPLFRNRILNNALIIKHRLRSDDIFLFDEARPTATKIIIPFDRSDLGLGGQS
                     FFVGQRGWVELLREACNDHTDMTRDLATLRMIDMLPSLDPFLLREHLRRHGMNIAACY
                     FALSPSDLEQMQGFVSFEISRLIELAYRDTGRFSGAHAARLVQALLSTDVDERLEPLR
                     ETLVMQGESFKEGVFSWKGFLYYKWMLTRLWPQLSVVTGEIGQLVIAGARDTETARYL
                     DEARKRLQAGVLIERNAVQRTIKIYDDAFEDLIENGKPQAFREFLLRAPDMFLSLGEK
                     VGVISHIASYWRYRFPEGRIPTAHVDEAIDIFQDFEGGLSVNLTS"
     gene            complement(2983..>3789)
                     /locus_tag="PLH31_05310"
     CDS             complement(2983..>3789)
                     /locus_tag="PLH31_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88679.1"
                     /translation="RVRQILLNLVSNALKFTETGRVDIRIAPRATGGIELVVSDTGVG
                     LTPDQQGRLFSPFSQGDLSTARRFGGTGLGLAISRQLAMLMGGEITVTSTFGQGSAFT
                     VWLDLPQAQAPVLRSAQEADLDLNGARVLVVDDNVVNQAVARAILESARVTVATAEDG
                     RAALARLRVEDFDLVLMDVHMPRMDGVEAVRRIRAGEGGRVDIPILALTADAMAGEAE
                     RLLKQGFDDAHPKPVQPGELLRAVSRLRRGSYGGQAARIHRGASTQSLCG"
BASE COUNT          659 a         1253 c         1236 g          641 t
ORIGIN      
        1 aagaatttgg cgataacgac tatgtcggca atcccagcct gaagccggaa acggcctggg
       61 gcctggacgt gggttacgag cgtcgcctgg gcgcgcgcgg cgtggccggc gtcaacgtct
      121 tctaccgcga cgtcaccgac ctgatcgagc tgaccagcat cggcacgggc tcggccggcg
      181 cgccggccct gctgctgcag acccagaacg tcggcgacgg ctcggtgatg gggatcgagt
      241 tcgacctctc caccccgctg accgtcttcg ggctggataa caccggcgtg ttcttcaact
      301 attcctggct cgacagcgac atcgacgaca ccttcggcag ccgcaagttc aacagccagt
      361 cggactatgt gatgaacgtc ggcttcatcc aggacctgcc caccttcggc ggcgccttcg
      421 gggccaccta tcgcaagcag ggcgaagccg atggccggat catcggcgaa gaggtcacca
      481 cctcctacgg cgctgacctg gaaatcttcc tggaaaagcg ttttggcgag aagttcacgg
      541 tgcgcctgac cggctccaac ctgctgaacg ccaagaagga agaagtcttc aacaagttta
      601 acagcaccgc cgatcaggtt tcgcgggact ttgaccaata cgagctggag agcgaagagg
      661 ccggtccggt cttccaactg atcgcccgca tggcgttcta gagcattttc cgataagtgt
      721 gaaacggtta tcggatcgaa aaatgctcta aacttttgat ttagagccct tttcgcccga
      781 ccctgatgac gccatcaggt cggaaagggc tctagggaag accttccgga aacgaagaag
      841 ggcggcgtcc tcgcggacgc cgcccttttt tgttggcggg gcgttcaggc cgtggtcagg
      901 ccgcgccccg gcctccgtcg tcatcggcgg ccatgaaacc cagcagcgag tcgatgcgcg
      961 ccagcagggc ggcgcgggcc tcagcccccg gggcctcgct cttcatgcag tcttccaggg
     1021 cgacggcggc cgcgaccagg cgcggctcct tgacggcgtc gcccaccttg cgcagttcca
     1081 gcgcctgggc ggccagggcg cggcgagcct gcatgggatc gctgtccagc gccccggcgg
     1141 ccgagcggac gatgcgcagc gcctgcgaca ggcgagccgc gaccgtgccg gccgcatcgg
     1201 ctttgcgctt gcgcgggccc ttgtattcgg ccgagttgaa gcggcgacga tcgggaccga
     1261 catattgcac cgcctcgacc cagtcgcgcg gcttgagggt cacggcctcc aggcggcgct
     1321 ccagatcgcc gatcgtgaag ggcttgcgca tgaactcgtg gatgccagcg tcacgggccc
     1381 cgaagatcgc ctcggccgtg gcttcggcgg tgcacatgat gatcggcgcc tcgcggcaga
     1441 ccagatcgct gcgacggatc ttcctggtca gcatcaggcc atccacgccg gcgctggcgt
     1501 gctcgacaaa gatcagttgc ggatcaaccg cgcgggccat cgcgtagccc ttctcgatgg
     1561 tcggagcggg atagatctgc acgttgccca gctggcgcag ctggtcagcc agcagcttgg
     1621 ccgtcgccgg attggcgtca acgaccagca cccgctgaac cttggcggcg acgcgctgaa
     1681 tggtgcggag attgccgtcg aacaccggag aatcggctcc tgataaggtt actaggccca
     1741 gaccctaggc cgggaacgta aaaatggggt tctcagacgc cgcgtcagct tgtcaggttt
     1801 acgctaagtc cgccctcgaa atcctggaag atatcgatgg cctcgtccac atgggcggtc
     1861 ggaatacgcc cctccgggaa ccgatagcgc cagtagctgg cgatgtgcga aatcaccccc
     1921 accttttcgc ccagcgacag gaacatgtcg ggcgcgcgca gcaggaattc gcgaaacgcc
     1981 tgcggcttgc cgttctcgat caggtcctcg aacgcgtcgt catagatctt gatggtccgc
     2041 tgcacggcgt tgcgttcgat cagcacgccg gcctggagcc gcttgcgggc ctcgtccaga
     2101 tagcgggcgg tctcggtgtc gcgcgccccg gcgatgacca gctggccgat ctcgccggtc
     2161 accaccgaca gctgcggcca caggcgggtc agcatccact tgtaatagag gaagcccttc
     2221 cagctgaaga cgccttcctt gaagctttcc ccctgcatga ccagggtctc gcgcaggggt
     2281 tccagccgct catcgacatc ggtcgacagt agggcctgga ccaggcgcgc cgcatgggcg
     2341 cccgagaagc ggcccgtatc gcgataggcc agctcgatca gccgcgagat ttcgaagctg
     2401 acgaagccct gcatctgctc cagatcgctg ggcgacaggg cgaagtagca ggcggcgatg
     2461 ttcatgccgt ggcggcgcag atgctcgcgt agcaggaacg ggtccagcga cggcaacatg
     2521 tcgatcatgc gcagggtcgc cagatcccgg gtcatgtcgg tgtggtcgtt gcaggcctcg
     2581 cgcagcagct cgacccagcc gcgctgcccc acgaagaacg actggccgcc cagacccagg
     2641 tcactgcggt cgaaggggat gatgatcttg gtcgccgtcg gccgcgcctc gtcgaacaga
     2701 aagatatcgt cgctgcgcag gcggtgcttg atgattaggg cgttgttgag aatccggttg
     2761 cgaaacagcg gatgctgggc gtgctcgggc ctgtggccgc tgtcggcctg gatggccagc
     2821 aggttcagaa cccggctggt cgaagccgtc cgctccagcg cccgaaggct gcgggtcacg
     2881 cggtcattgc tgcgcaccgg cttgagttta cgacgcgctg acacggtgtg gcggtccctt
     2941 ttgggtaggc gtttcttcta tcggcgccga actaaacaat cgctaaccgc acagggattg
     3001 cgtgctcgcg ccacggtgga tacgcgccgc ctgcccgcca taggatccgc gccgcagccg
     3061 cgacaccgct ctgagcagct cgcccggctg gacgggcttg ggatgggcgt cgtcaaagcc
     3121 ctgcttgagc agtcgctcgg cctcgcccgc catggcgtcg gcggtcaggg ccaggatcgg
     3181 gatgtccacc cgccccccct cgccggcccg aatgcgccgc acggcctcga cgccgtccat
     3241 ccggggcatg tggacgtcca tcagcacaag gtcgaaatcc tcgaccctca ggcgggccag
     3301 ggcggcccgg ccgtcttcgg cggtggccac cgtcacccgg gcggattcca ggatcgcccg
     3361 ggccacggcc tgattgacca cattgtcgtc caccaccaga acccgcgcac cgttcagatc
     3421 caggtcggcc tcctgcgccg agcgcaggac gggagcctgg gcctgcggca ggtccagcca
     3481 gaccgtgaag gcagagccct ggccgaacgt actggtcacg gtgatctcgc cgcccatcag
     3541 catggccagc tgacggctga tcgccaggcc caggcccgtg ccgccaaacc ggcgcgccgt
     3601 cgacaggtcg ccctggctga acgggctgaa cagccggccc tgctgatccg gggtcagacc
     3661 gacgccggtg tcggacacca ccagttcgat gccgcccgtg gcgcgcggcg cgatacggat
     3721 atcgacccgg cccgtttcgg tgaacttcag ggcgttggac accaggttca gcagaatttg
     3781 ccgcacccg
//
LOCUS       DAOMEO010000177         3782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2821_length_3782_cov_6.141132, whole genome shotgun sequence.
ACCESSION   DAOMEO010000177 DAOMEO010000000
VERSION     DAOMEO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_3782_cov_6.141132"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLH31_05315"
     CDS             <1..756
                     /locus_tag="PLH31_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HQR88680.1"
                     /translation="GQIPTPSGIIDLSNSVENEHYCLKLLEAFGLTVAKTEIVRFGAR
                     TVLVVERFDRAWRDTQLLRLPQEDFCQALSTPPSQKYQNQHGPGAVDILRLLKASDTP
                     QEDQAAFFKSQILFWLMGATDGHAKNFSLFLKPNGRFSMTPFYDVLSAQPAFDAKAIP
                     HRAYTLAMSAGESRHYKILDIHGRHFAETATEAGLRPHMVRRVIEEIFDLAPVACERA
                     LAQMPPGFHPETHNSIGGAIAHRLPRLRTALQA"
     gene            961..1224
                     /locus_tag="PLH31_05320"
     CDS             961..1224
                     /locus_tag="PLH31_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88681.1"
                     /translation="MAETAQSTELELHPILKATGQKLLSAIPVGGLATAGGKGAFSVS
                     VSPQQATRALLVLSNLLQAMGTQGWSVIRSDKGLQLGLNVALR"
     gene            1325..1648
                     /locus_tag="PLH31_05325"
     CDS             1325..1648
                     /locus_tag="PLH31_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR88682.1"
                     /translation="MGPERRRRWSEATKRAVLEAAFAPGAVVIEIARQHEISTSLIYK
                     WRQEAMAAVAAPAFAPTILLDEPAPATSRPEPAIVVELVGGARVSINADASPGLVAAT
                     LRALR"
     gene            1645..1992
                     /gene="tnpB"
                     /locus_tag="PLH31_05330"
     CDS             1645..1992
                     /gene="tnpB"
                     /locus_tag="PLH31_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HQR88683.1"
                     /translation="MIPIASGVRVWIAAGHTDMRKGMLGLALLVQEGLGRDPFAGDVF
                     VFRGRGGSLIKALWHDGLGLSLYAKRLDRGRFVWPVSADGAVALTAGQMSYLLEGIDW
                     RNPQHTWRPSAAG"
     gene            2063..3751
                     /locus_tag="PLH31_05335"
     CDS             2063..3751
                     /locus_tag="PLH31_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQR88684.1"
                     /translation="MDADLAALPDDIEALKAALLVARAEAVQAQDVAARAQAEAARAQ
                     AEAAEAKARVSDDQALIAHLKLQIQKLNRERFGPGSERTARLLDQLELELEELEASAT
                     DDELAAEMAAARATTVAAFSRKRPSRQPFPEHLPRERVVVPGPTACACCGGLRLSKLG
                     EDVTETLEVVPRSWKVIQHVREKFSCRDCEAIGQAPAPFHVIARGWAGPSLLAMILFE
                     KFGQHQPLNRQADRYAREGVPLSLSTLADQVGACTAVLAPLFKRLEAHVLAAERLHGD
                     DTTVPVLAKGKTDIARLWVYVRDDKPFAGSAPPGAVFYYSRDRGGEHPQAHLSGYAGL
                     FQADAYGGYGKLYEPGRNPGPILEAACWAHARRPFFVLADLEQNARRKARGAAPAVIS
                     PIALGMVQRIDALFEIERGINGQDADRRLAARQDLSAPLIAEMEIWMREQRAKLSRGH
                     DLARAFDYMLKRWAAFTRFLDDGRVCLSNNAAERALRGVAMGRKSWLFCGSDRGGQRA
                     AVMYSLIVTAKLNDIDPQAWLADVLARIAEHPSQQLDDLLPWNWRPLTTVGRAA"
BASE COUNT          656 a         1229 c         1244 g          653 t
ORIGIN      
        1 ggacagatcc caacgccctc cgggatcatt gacctctcca acagcgtcga aaatgaacac
       61 tactgcctga agctactgga ggctttcggc ttgacggtcg ccaagaccga gatcgtgcgc
      121 ttcggcgcgc ggaccgtcct cgttgtcgaa cgcttcgacc gcgcctggcg tgacacccag
      181 ctcctgcgct tgccgcagga ggacttctgc caggcgctgt ccacgccgcc atcccagaaa
      241 tatcaaaacc agcacggacc cggcgccgtc gacatcctgc gattgctcaa ggcgtccgac
      301 acgcctcaag aggatcaggc cgccttcttc aaaagccaga tcctcttctg gctgatgggg
      361 gcgaccgacg ggcacgccaa gaacttcagc ctgttcctca agccgaacgg ccggttttcc
      421 atgacgccgt tttacgatgt gctgagtgct caacccgcct tcgacgcaaa agccattccc
      481 catcgcgcct acacgctggc gatgtcggcg ggcgagagca gacactacaa aatcctcgac
      541 attcacggac gtcacttcgc cgagaccgcc acggaagccg gcctgcgccc ccacatggtg
      601 agacgggtga ttgaggaaat cttcgacttg gcgcctgtcg cctgtgagcg agcgctcgcc
      661 caaatgccgc ccgggtttca cccggagacg cataacagca tcggcggggc gattgcccat
      721 cgcttgcccc gcctgcgcac ggcactgcag gcctgaactt cacctcggag gttcgagctc
      781 gaccggcgac tgtcgcaaac gccccattcg tgagtgccgc cacctcttgc atttcttgac
      841 ccgtttcggc gacacatggc ggatgccaaa gacctacgcc gaactttgca ggagcttgcg
      901 cgccaccgac gagaggggac agcctacgcg gttcaaccgg cgccgagctc aataatcatt
      961 gtggccgaga ctgcacagag caccgagctc gaactgcatc cgatcctgaa ggccactggc
     1021 cagaagttgc tctcggccat cccagttggc ggccttgcca cggcgggcgg caagggagcg
     1081 ttttcggtgt ccgttagtcc gcagcaagca actcgggcgc ttcttgttct cagcaatctc
     1141 ctgcaggcga tggggaccca aggatggtcg gtcatccgct cggataaggg cttgcagctc
     1201 ggccttaacg tagccctccg gtgaaggccg ccttgccgag gtgtctggga tgctgatgct
     1261 gtgctcgtaa ggacgtcctt acgagtacgc aaagctgcag ggcatgggcc attcgagttt
     1321 gattatgggc ccggagcgcc gtcggcgttg gtcggaggca actaaacgcg ccgtgctgga
     1381 agcggcgttc gcgcccggcg cggtcgtgat tgagatcgcc cggcaacacg agatctccac
     1441 cagcctgatc tacaagtggc gacaggaagc gatggcggcg gtcgccgcac cggctttcgc
     1501 gcccacgatc ctgctcgatg agccggcgcc ggcgacatcg cgtcccgagc cggcgatcgt
     1561 ggttgaactg gtcggcggcg cgcgggtgag tatcaacgcc gacgcctcac ccggcttggt
     1621 cgcagcgacg ctgcgagccc tccgatgatc ccgatcgcct ctggggtgcg cgtgtggatc
     1681 gcggccggac acaccgacat gaggaaggga atgctgggcc tggcgctgct cgtacaggaa
     1741 ggcttgggtc gcgatccgtt cgccggcgat gtcttcgtgt tccgagggcg tggcggatcg
     1801 ctgatcaagg cgctgtggca tgacggcctg gggctgtcgc tctatgccaa gcggctggat
     1861 cgaggacggt tcgtgtggcc ggtcagcgcc gatggcgcgg tggcgctgac ggcggggcag
     1921 atgtcgtatc tgctggaggg catcgactgg agaaaccccc agcacacctg gcgtccgtct
     1981 gccgccggat agccaaaaat agtcggggcg ccctgcattt aggggcgcca caagcagcga
     2041 aatttgtgat tccatctggg tcatggacgc tgacctcgca gccctgccgg acgatatcga
     2101 agcgctgaag gcggcgcttc tggtcgcccg agccgaggcg gtgcaagcgc aagacgtggc
     2161 tgcgagagct caggctgaag ccgccagagc ccaggctgaa gccgctgagg ccaaagcgcg
     2221 cgtgtccgac gaccaggcgc tgatcgccca tctgaagctc cagatccaga agctcaatcg
     2281 tgagcgcttc ggccctggct cggaacgcac ggcccgtctg ctggatcagc tggaactaga
     2341 gttggaggag ctggaggctt cggcgacgga cgacgagctg gccgccgaga tggcggcggc
     2401 tcgggccacg accgtggcag ccttcagccg caagcggcct tcacgccaac ccttccccga
     2461 gcacctgccg cgtgagcgcg tagtcgtgcc gggtccgacc gcctgcgcct gctgtggcgg
     2521 actgcggctc tcgaagctgg gcgaagacgt caccgaaacg ctggaggtcg tgccccggtc
     2581 ctggaaggtc atccagcatg tgcgcgagaa gtttagctgc cgtgactgtg aggccatcgg
     2641 tcaggcgccg gctccgttcc atgtgatcgc ccggggatgg gcggggccca gcctgctggc
     2701 gatgatcctg ttcgagaagt ttggtcagca tcagccgctc aatcgccagg ccgaccgcta
     2761 tgctcgcgag ggcgtgccgc tcagcctgtc gaccctagcc gaccaagtcg gagcctgcac
     2821 ggcggtgctg gcgccgctgt tcaaacggct ggaggcgcac gtgctggcgg ccgagcgatt
     2881 gcacggcgac gacaccacgg ttccggtgtt ggccaagggc aagaccgaca tcgccaggct
     2941 ctgggtctat gtgcgcgatg acaagccgtt cgccggatcg gcgccaccgg gcgcggtctt
     3001 ctactactcg cgggatcggg gcggcgaaca cccacaggcg catctgtcag gttatgccgg
     3061 cctgttccag gccgacgcct atggcggcta cggcaagctc tatgagccag ggcgaaaccc
     3121 aggtcccatt cttgaagcag cctgctgggc acacgcgcgt cggccgttct tcgtgctggc
     3181 cgacctggag cagaacgcgc gccgcaaggc tcgcggcgct gcgccggcgg tgatctcgcc
     3241 gatcgccctg ggcatggtcc agcggatcga cgcgctgttc gagatcgagc gagggatcaa
     3301 cggccaggac gctgatcggc gccttgcggc gcggcaggac ctcagcgccc cgctgatcgc
     3361 cgagatggag atctggatgc gcgagcagcg cgccaagctc tcacgcggtc atgacctggc
     3421 ccgagccttt gactacatgc tcaagcgctg ggccgcgttc acgcgcttcc tcgatgatgg
     3481 ccgcgtctgt ctgagcaaca atgccgccga gcgggcgctg cgcggcgtgg caatgggacg
     3541 caagtcctgg ctgttctgcg gctccgatcg cggcggtcag cgcgcggcgg tgatgtacag
     3601 cctgatcgtc accgccaaac tgaacgacat cgatccccaa gcctggctgg ccgacgttct
     3661 ggctcgcatc gccgagcatc ccagccagca gcttgatgat ctgctgcctt ggaactggcg
     3721 gccgctcacc acggtcgggc gcgccgctta gacgccagcg cggccttcac cggatgggta
     3781 ct
//
LOCUS       DAOMEO010000178         3770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2830_length_3770_cov_2.704711, whole genome shotgun sequence.
ACCESSION   DAOMEO010000178 DAOMEO010000000
VERSION     DAOMEO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_3770_cov_2.704711"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /gene="gspD"
                     /locus_tag="PLH31_05340"
     CDS             complement(<1..2081)
                     /gene="gspD"
                     /locus_tag="PLH31_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HQR88685.1"
                     /translation="MTKAHSPRASVLAIAAAIALAGASLPAASVRAQTQVLNVQDADI
                     RVFIQDVAKSTGTTFIIDPRVKGTVTVASNGPLNRRELFEVFLATLRANNFVATPAGN
                     GAYRIEPSENAARQPSTAGGQFATEVFRLRTIDAASAVDMLKPLIGPQGQVVANPRGG
                     AIVVADYADNVRRIRALLAQVDQDRASVHTVTLTNSSAREIAQVINEIMAAPGADGKG
                     GRGAVTVVAVESSNSVLLRGDSDAVQRLLPIITDLDRRAESSDDVRVVFLRHANAEQM
                     LPVLQQLVGQSSTASAGTSARSLGATRSAGSAATASSASAAPAASSSPVAASAGGAQA
                     SVARYPGANALIISAPPETQRTLAEVIRQLDIRREQVLVEAIVVEVSDDAAKQLGVQF
                     LLGGADGTVPFAATNYTNASPSLLPLVAAGAAGDPKDTDSDALVSLRDAAVSSLLTAS
                     GITSGIAGRSGDTLFGAIINAVKKDAASNLLSTPSIMTLDNEEARILVGQEVPITTGE
                     VLGDSNSNPFRTIQRQNVGVQLEVKPQINAGGGITLFLRQEVSSVAGPVSVGSSELII
                     NKREIETTALVDDGDIVVLGGLLDQQETVSVQRTPLLSDIPGLGALFRSTARERGKTN
                     LMVFIRPRIIRSVTDARNVTAPRYDYMRNLPAMTTPDGDNSLDAVIRDYLRTAPPTAV
                     APAPVASGPIAA"
     gene            complement(2078..2473)
                     /locus_tag="PLH31_05345"
     CDS             complement(2078..2473)
                     /locus_tag="PLH31_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HQR88686.1"
                     /translation="MLELVLLLVVATQGARLIWIVAAPTAPAAAPVAIQKARADPGVL
                     ASFDAFGVSGGVSAVSGGQGAGEFRLFGVRAGGGGASAIIAGPDGVQKAYAVGEQVAD
                     GVILTSVAADHVELSRNGARSTLSFPEIQ"
     gene            complement(2684..>3770)
                     /locus_tag="PLH31_05350"
     CDS             complement(2684..>3770)
                     /locus_tag="PLH31_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88687.1"
                     /translation="TFTETTGGDGAAAYEASLKVHAAYAQVDAEFLPRLRTSFGLRLE
                     DAEQEVQVVDLFGGVPAAPTSLTETYVLPAATLTYNFYEDMQFRLGASKTIARPQFRE
                     LAPQQYFDPDSDRLFIGNPYLKDTELVNLDARVEWYFNAGQYLSGGLFYKDIDSPVEA
                     VVSEQGATIVQTYVNAPKATLYGLEVDAKKYVDLPFEGFLFDGKRWLIGANYTYSKSE
                     VKVSNGDVVFPLAGAGASRPATDYVKDGSQLQGLSEHLANIQFGYENAEARSQATVLA
                     TYVSERISARGRPGQPDLIQDPGVSVDFTYRRAFDLMEREVEFTFKARNILGEDYEEY
                     QELGGGRVDNNSYDLGQSFTVSLSAKF"
BASE COUNT          637 a         1200 c         1306 g          627 t
ORIGIN      
        1 gcggcgatcg gacccgaggc cacgggcgct ggcgcgaccg ctgttggggg agcagttcgc
       61 agatagtcgc ggatcaccgc gtccagcgag ttgtcgccat ccggcgtggt catggccggc
      121 agattgcgca tgtagtcgta acgcggcgcc gtgacattgc gggcgtccgt gacgctgcga
      181 atgatccgcg ggcgaatgaa aaccatcagg ttggtcttgc cgcgttcccg cgccgtgctg
      241 cggaacaggg cgcccagacc agggatgtcg ctgagcagcg gcgtgcgctg caccgagacc
      301 gtctcctgct ggtccagcag gccgcccagc acgacgatgt cgccatcatc gaccagagcc
      361 gtggtctcga tctcgcgctt gttgatgatc agctcgctgg agccgacgct gactggcccg
      421 gccacgctgg aaacttcctg gcgcaggaac agggtgatgc cgccgccggc gttgatctgc
      481 ggcttgacct ccagctgaac gccgacgttc tggcgctgga tggtgcggaa ggggttggag
      541 ttggagtcgc ccagcacctc gccggtggtg atcggcactt cctggcccac caggatacgg
      601 gcttcctcgt tgtcgagggt catgatcgac ggcgtcgaca gcaggttcga cgccgcgtcc
      661 ttcttcaccg cattgatgat ggcgccaaac agcgtgtcgc cgctgcggcc ggcgatgccg
      721 ctggtgatgc ccgaggcggt cagcagcgaa ctgaccgccg catcgcgtag cgacaccagg
      781 gcgtcagagt ccgtatcctt ggggtccccg gcggcgccgg cggcgaccag gggcagcagg
      841 ctcggcgagg cgttggtgta gttggtggcg gcgaagggaa ccgtgccgtc ggcgccgccc
      901 agcaggaact gcacgcccag ctgcttggcg gcgtcgtcgg atacttccac gacgatagcc
      961 tcgaccagca cctgttcgcg gcggatgtcg agctggcgga tcacctcggc cagcgtgcgc
     1021 tgggtctcgg gcggcgcgct gatgatcaga gcattggccc ccgggtagcg cgcgacactg
     1081 gcctgggcgc ccccggccga ggcggccacg ggcgaagagg aggccgcagg cgcggcgctg
     1141 gcggagcttg cggtcgcggc gcttcccgcc gaacgggtcg cgccgagcga ccgggccgaa
     1201 gttcccgccg acgccgtcga actctggcct accagttgct gcaggaccgg cagcatctgc
     1261 tcggcattgg cgtggcgcag gaacaccacc cggacgtcgt cgctggactc ggcgcgacga
     1321 tccaggtcgg tgatgatcgg gagcagacgc tgcacggcgt cggagtcgcc ccgcagcagc
     1381 accgagttgc tgctctcgac ggcgaccacg gtcaccgcgc cgcgaccgcc ctttccgtcc
     1441 gcgccgggcg cggccatgat ctcgttgatg acctgggcga tttcacgcgc tgagctgttg
     1501 gtcagggtca cggtgtggac gctggcgcgg tcttgatcga cctgggccag caacgcgcgg
     1561 atgcggcgca cattgtcggc gtagtcggcc acaacgatgg ccccgccgcg cggattggcc
     1621 accacctggc cctgcggacc gatcagcggc ttgagcatgt ccacggcgct ggcggcgtct
     1681 atggtccgca ggcggaagac ttcggtggcg aactgccctc ccgccgtgct gggctggcgg
     1741 gcggcgtttt cagaaggttc gatgcgatag gcgccattcc ccgccggcgt ggcgacgaaa
     1801 ttgttggcgc gcagggtcgc caggaaaacc tcgaacagct cccggcgatt gagcggtccg
     1861 ttgctggcga ccgtcactgt gcccttgacc cggggatcaa tgatgaaggt cgtgcctgtg
     1921 ctcttagcga cgtcctggat gaagacgcga atatcggcgt cctggacgtt cagaacctgg
     1981 gtctgggcgc gaaccgacgc tgcaggcaaa gacgcgccgg ccagtgcgat ggccgcggcg
     2041 atggccagga cggaagcgcg cggcgaatga gccttggtca ttggatttcc gggaacgaga
     2101 gggtgctgcg cgcgccgttg cgggagagtt cgacgtggtc ggcggccacg ctcgtcagaa
     2161 tgacgccgtc ggccacctgt tcgccgaccg cataggcctt ctggacgccg tccggcccgg
     2221 cgatgatcgc cgacgcccca ccgccgccgg cgcgcacgcc gaaaagccgg aactcgcccg
     2281 cgccctggcc gccagagacg gcgctgacgc ctccagagac accgaaggcg tcgaagctgg
     2341 ccagcacgcc ggggtcagcg cgcgcttttt gaatggcgac cggcgcggcc gccggcgccg
     2401 taggcgcggc gacgatccag atcaggcgcg cgccctgtgt ggcgacgacc agcagcagga
     2461 ccagctcaag caccagaagg atcctggcgt tcggcgcacg gagataatct gtgaggttca
     2521 agggatcctg ccgatacgcc gcttgcgaca tgggcggagt atcccggcgc ggatgcagaa
     2581 cggcgacagt ttgacgacag tatgatgaca aacgctcccc actcggacga atggggagcg
     2641 ttgccgacgc cacgataaga ccccttaggg ccttgaagtt tctctagaac ttggccgaga
     2701 ggctgaccgt aaagctctgc cccaggtcgt aggaattgtt gtcgacgcga ccgccgccaa
     2761 gttcttgata ttcctcgtag tcctcaccga ggatgttacg agccttgaag gtgaactcga
     2821 cttcgcgctc catcagatca aaggcgcgac gataggtgaa gtcgacggac acgcccggat
     2881 cctggatcag gtcaggctga cccggacgac cgcgcgccga gatgcgctcg ctgacatagg
     2941 tggccagcac ggtggcctgc gaccgcgcct cggcgttttc gtagccgaac tggatgttgg
     3001 ccagatgctc ggacagaccc tgcagttggc tgccgtcctt gacatagtcg gtggcgggac
     3061 gcgaagcccc ggcgccggcc agcgggaaga ccacatcgcc attcgacacc ttcacctcgg
     3121 acttcgagta ggtgtagttg gcgccgatca gccaacgctt gccgtcgaac aggaagcctt
     3181 cgaagggaag gtcgacgtac ttcttggcgt ccacttccag gccatacagc gtcgccttgg
     3241 gagcgttcac ataggtctgg acgatggtag cgccctgctc gctgaccacg gcttcaaccg
     3301 ggctgtcgat atccttgtag aacaggccgc ccgacagata ctggccggca ttgaagtacc
     3361 actcgacgcg agcgtcgagg ttcaccagct cggtgtcctt caggtacggg ttgccgatga
     3421 acaggcggtc gctgtccgga tcgaagtact gctgcggcgc cagttcgcgg aactgcgggc
     3481 gggcgatggt cttggaggcg ccaagacgga actgcatgtc ttcatagaaa ttgtaggtca
     3541 gcgtcgccgc cggaagcaca taggtttccg tcagcgaggt cggcgccgcc ggcacgccgc
     3601 cgaacagatc cacgacctgc acttcctgct cggcgtcttc cagacgaaga ccgaacgagg
     3661 tgcgcaaacg cggcaggaac tcggcatcga cctgggcata ggccgcatgg accttcagcg
     3721 aggcctcata ggccgccgcg ccgtcgccac cggtggtttc ggtgaaggtc
//
LOCUS       DAOMEO010000179         3769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2833_length_3769_cov_2.208401, whole genome shotgun sequence.
ACCESSION   DAOMEO010000179 DAOMEO010000000
VERSION     DAOMEO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_3769_cov_2.208401"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLH31_05355"
     CDS             complement(<1..169)
                     /locus_tag="PLH31_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQR88688.1"
                     /translation="MALVFDKPADLIGKEGTKLGSTDWLTIEQDRVDGFAEVTGDHQW
                     IHVDVERAKAGP"
     gene            complement(172..1110)
                     /locus_tag="PLH31_05360"
     CDS             complement(172..1110)
                     /locus_tag="PLH31_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR88689.1"
                     /translation="MTRSFGVSLLACAALLSGPARAERPPSYLGVDISYANEMDDCGA
                     VYRSGGKVVEQYAFFKSAGANVARIRIWNDPTWTPYSNLRDVKKSIARARAAGLQVLL
                     DFHYSDDWADGDKQLAPKAWETLSTPDQAKALYAFTHDTLTTLDREGLMPDLVQVGNE
                     TNGEIVSSLARAKDPINWERNALLFNAGIKAVRDAGAGAKIKPRVMLHIAQPENVEPW
                     FAAAAKAGVMAFTRCTALEAADHGVRINAVAPSLAMHPFLAKVTTEEMLEKLVKTEAY
                     GRPAEVWEVANVMLFLASDLSSYMTGEIVPVSSQKA"
     gene            complement(1203..>3360)
                     /locus_tag="PLH31_05365"
     CDS             complement(1203..>3360)
                     /locus_tag="PLH31_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQR88690.1"
                     /translation="EPNPHCRDKLLRLPFERHEVAASNAAGKGELFLSKTWLQSTGSS
                     LYRENTDYFDDETAIRHEVDKARIDDLLAGRSFDFVKIDTQGSELDVLVGGEAVLARA
                     DYIQIEVSLVDYNIGGAMAEDVFAKMAQMGFKCADVMEFNRMPEVRDNALLQIDVLFE
                     RADRLRRPETSSTGGRQDEVLALAERLFNEDRPEDALVLFEHLLGGAHDYAAWIGVSR
                     SHLAGGRVLEALRALVKAKALTPDIHALWPLIQQQTSLGSQAFNRHLNAGEVVLAEPY
                     ASVMADLMPGGAQMVGAAMGCNQALGRMDEALNFARRLIVLDPGHVGALTLLADEARA
                     AGDHPTEIRHRAVLAVSPDKDIHPLVRLRDFHEAMSLLLCRPLSDADIGLLTHLAAEV
                     PKVAVGDLLADPEWADWVHHYRSLLGSLDIGSITGATPAPTVDAPARYLTATGEALDA
                     AGLKAMADRLGIRAVFFAAADEKYVDQYGRWYALSVLRHSDVSSLVVIHVIGGAERLA
                     EAVKAVGVKDERLVFVGDDFDASAVTTRCYDAPPKGLIALPVAHFQSVRFQRLGGLLE
                     TLGRPIFVSDIDLLLQDGVSDLLEAWARADVVFNENWKNLAAGSRITANLLLVNPTPN
                     AARLFNFLRAYLDDRLGRETVTRWIDQVALLLGRHHLTRHSPDAVLGCFDTTKDINNI
                     MFESYQEHPFRFLSLYHGFDTSSLENDSRVLGSDPV"
     assembly_gap    3361..3370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3371..3509)
                     /locus_tag="PLH31_05370"
     CDS             complement(<3371..3509)
                     /locus_tag="PLH31_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88691.1"
                     /translation="MNRQHLEALRREGYAPRTLLDVGAHLGEFTQGFLEVFPGCLPTL
                     IE"
     gene            complement(3520..>3769)
                     /locus_tag="PLH31_05375"
     CDS             complement(3520..>3769)
                     /locus_tag="PLH31_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine transferase"
                     /protein_id="HQR88692.1"
                     /translation="EDLFHDMVAEYQAGQRPTPNIANLEAYLEIGIELDRDDREMLTE
                     VDFESLYKTALTRRHLARPLGPDNRLWTAEDIAAAERR"
BASE COUNT          623 a         1269 c         1228 g          639 t
ORIGIN      
        1 acggtcccgc cttggcgcgt tcgacatcga cgtgaatcca ctggtgatcg ccggtaactt
       61 ctgcaaagcc gtccacccgg tcctgctcga tggtcagcca gtcggtcgac cccagctttg
      121 tgccttcctt gccgatcagg tcggcgggct tgtcgaaaac cagcgccatc gtcatgcctt
      181 ctggctcgaa accggcacga tctcgccggt catgtaggat gacaggtcgc tggcgagaaa
      241 cagcatgaca ttggccactt cccacacttc ggcggggcgg ccataggcct ctgtcttgac
      301 cagcttttcc agcatttcct cggtcgtcac cttcgccagg aacgggtgca tggccaagct
      361 gggcgccacc gcattgatgc gcacgccgtg gtcggcggct tcgagcgcgg tgcagcgggt
      421 aaaggccatc acgcccgcct tggccgccgc cgcgaaccac ggctcgacat tctcgggctg
      481 ggcgatgtgc agcatgaccc ggggcttgat cttcgcgccg gctccggcgt cgcgcacggc
      541 cttgatgccg gcgttgaaca gcagggcgtt gcgctcccag ttgatcggat ccttggcccg
      601 cgccaggctg ctgacgatct cgccattggt ctcgttgccc acctgaacca ggtcgggcat
      661 caggccttcg cgatccagcg tggtcagggt gtcatgggtg aaggcgtaca gcgccttggc
      721 ctgatcgggc gtcgacagcg tctcccaggc cttgggcgcc agttgcttgt cgccgtcggc
      781 ccagtcgtcg gaataatgga agtccagcag cacctgcagg ccggccgcgc gggcccgggc
      841 gatgctcttc ttgacgtccc tgaggttgga atacggcgtc caggtaggat cattccagat
      901 ccggatccgc gccacattgg cgcccgccga cttgaagaag gcgtactgct ccacgacctt
      961 gccgcccgag cgatagaccg cgccgcagtc gtccatttcg ttggcgtagg agatgtcgac
     1021 gcccagatag gacggcggcc gctcggcccg cgccgggccg cttagcaggg ccgcacaggc
     1081 cagaaggctc acgccgaaag aacgggtcat gctcatgctc catggtcgcc ggggcgaaaa
     1141 cgtcggcgtc gttatgggca catttatagt atcaatcaat catcatcgcc cgcaccgcgc
     1201 gctcagacgg ggtcgctacc cagaacccgg gaatcgttct ccaggctcga cgtatcgaag
     1261 ccgtgataga gcgacaggaa gcggaacggg tgctcctgat agctctcgaa catgatgttg
     1321 ttgatgtcct tggtggtgtc gaaacacccc agaactgcgt caggagagtg ccgcgtcagg
     1381 tgatgacggc ccagcagcag ggccacctgg tcgatccagc gggtgacggt ttcgcggccc
     1441 agccggtcgt ccagataggc ccgcaggaag ttgaacagcc gcgccgcgtt cggggtggga
     1501 ttgaccagca gcaggttggc ggtgatccgt gagccggcgg ccaggttctt ccagttctcg
     1561 ttgaagacga cgtcggccct cgcccacgct tccagcaggt cgctgacccc gtcctgaagc
     1621 aacaggtcga tgtcggagac gaaaatcggc cggcccagcg tctccagcag cccgccgaga
     1681 cgctggaagc gcaccgactg gaaatgggcc accggcaggg cgatcagccc cttgggcggc
     1741 gcgtcatagc agcgggtcgt cacggccgag gcgtcgaagt cgtcgcccac gaagaccagc
     1801 cgctcgtcct tgacgccgac cgccttgacc gcttcggcca ggcgctcggc gccgccgatc
     1861 acgtggatca cgaccaggct ggagacgtcg ctgtggcgca ataccgacag agcgtaccag
     1921 cggccgtact ggtcgacata tttctcgtcg gcggcggcga agaacacggc ccgtatgccg
     1981 agccggtcgg ccatggcctt caggccggcc gcgtccagcg cctcgcctgt cgccgtcaga
     2041 tagcgcgccg gcgcgtcaac tgtcggcgcg ggggtcgccc ccgtgatcga gccgatgtcc
     2101 agcgaaccca gcagggaccg gtagtgatgc acccagtcgg cccattcggg atcggccagc
     2161 agatcgccca ccgccacctt gggaacctcg gccgccagat gggtcagcag gccgatatcg
     2221 gcatccgaca gcggccggca cagcagcaga ctcatcgcct catggaagtc gcgcaggcgc
     2281 accagaggat ggatgtcctt gtcaggcgat accgccagga cggcccggtg gcggatctcg
     2341 gtcggatggt cgcccgccgc gcgggcctcg tcggccagca gggtcagcgc gccaacatga
     2401 cccggatcca gcacgatcag acggcgcgcg aagttcaggg cctcgtccat gcgccccagg
     2461 gcctggttgc agcccatggc cgccccgacc atctgggcgc cgccgggcat caggtcggcc
     2521 atcaccgacg catagggctc ggccaggacc acctcgccag cgttcaggtg ccggttgaag
     2581 gcctgggacc ctagcgaggt ctgctgctgg atcaggggcc acagggcgtg gatgtccggc
     2641 gtcagggcct tggccttcac cagagcccgc agcgcttcca gcacgcgccc gcccgccagg
     2701 tggctgcgcg acacgccgat ccaggcggca tagtcatgcg caccgcccag caggtgctcg
     2761 aacagcacca gggcgtcctc gggccggtcc tcgttgaaca ggcgctcggc cagggccagc
     2821 acctcatcct gccgaccgcc ggtcgatgag gtctccgggc gacgcaaccg gtcggcccgc
     2881 tcgaacagca catcgatctg caggagcgcg ttgtcgcgca cctccggcat gcggttgaat
     2941 tccatgacgt ccgcgcactt gaagcccatc tgcgccatct tggcgaagac gtcctcggcc
     3001 atggcgccgc cgatattgta gtcgaccagc gacacctcga tctggatgta gtcggccctg
     3061 gccagcaccg cctcgccgcc caccagcaca tccagttccg agccctgggt gtcgatcttg
     3121 acgaagtcga agctgcggcc ggccagcagg tcgtcgatcc gggccttgtc gacctcgtgc
     3181 cggatcgcgg tctcgtcgtc gaaatagtcg gtgttctcgc gatagagcga actgccggtc
     3241 gattgcaacc aggtcttgct gaggaacagc tcgcccttgc cggccgcgtt cgacgccgcc
     3301 acctcgtgcc gctcgaacgg cagccgcagc agcttgtcgc ggcaatgcgg gttgggctcg
     3361 nnnnnnnnnn gctcgatcaa ggtcggcagg cagcccggaa acacctccag gaacccttgc
     3421 gtgaactcgc ccagatgcgc gccgacgtcc agcagcgtcc ttggcgcgta accctcgcgc
     3481 cgcaaggcct cgaggtgctg gcggttcatc ggcggaccct catcggcgct cggcggcggc
     3541 tatgtcttcg gcggtccaga gccggttgtc gggtcccagc ggccgcgcca gatggcgacg
     3601 ggtcagggcc gtcttgtaca ggctttcgaa gtcgacctcg gtcagcattt cgcggtcgtc
     3661 gcgatccagt tcgatgccga tctccagata ggcctcgaga ttggcgatgt tcggagtcgg
     3721 gcgctggccg gcctgatact cggcgaccat gtcgtggaac aggtcctca
//
LOCUS       DAOMEO010000180         3760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2838_length_3760_cov_2.648853, whole genome shotgun sequence.
ACCESSION   DAOMEO010000180 DAOMEO010000000
VERSION     DAOMEO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_3760_cov_2.648853"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLH31_05380"
     CDS             <1..795
                     /locus_tag="PLH31_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQR88693.1"
                     /translation="GDTIEQIAVEKAGIIKPGAPAVSARQHEAAEDVIDTAAALAGVD
                     LTLMGRDFDAWNERGRLLVQTQDRLLDLPAPSLPGDHQFANAGLAVAALLALADPRID
                     EAAMSAGIAGTVWPARFQRLTAGPLAGKASAAGADLWLDGGHNPHAGQAVARALGDLA
                     ARDGRPVALISGLLANKDATGFFSPFASLKAQVFTVTFEGHAAASAAQTAAAAELAGL
                     RAHACDSVEDALAKALALTPTPHVLICGSLYLAGEVLAMSPETWPT"
     gene            906..1016
                     /locus_tag="PLH31_05385"
     CDS             906..1016
                     /locus_tag="PLH31_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88694.1"
                     /translation="MAKGQKKSNKEVRKPKADKKAAPASVSPFIVTSAKK"
     gene            complement(1087..1422)
                     /gene="trxA"
                     /locus_tag="PLH31_05390"
     CDS             complement(1087..1422)
                     /gene="trxA"
                     /locus_tag="PLH31_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQR88695.1"
                     /translation="MSTVTVTDETFERDVLQADGPVLVDFWAEWCGPCKQIAPALEQI
                     SEELADHVVVAKVNIEDSPTTPSRYGVRGIPTMMLFRGGQMTSMKVGAMPKQKILEWL
                     NEAGVQQPA"
     gene            complement(1534..>3760)
                     /locus_tag="PLH31_05395"
     CDS             complement(1534..>3760)
                     /locus_tag="PLH31_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR88696.1"
                     /translation="TNDFFVGAGATGWRKGRDNRTLFVVGDQKQSIYSFQGADPARLL
                     IETQGYIARIAEAGREGSAVPLSVSYRSTREVLSFVDALFEVPENRLGLMPSGGDLEP
                     HQAFRTDGPGCVDLWPLTRERPGEERDAWEAPLDAESEHSANRRLAEAIAAEIAGILD
                     RGDEVHDKEARTRRAAHAGDILILVRRRKVLFEEIIRALKRRGVPVAGADRLSLSAHI
                     AFDDLLALGRFVLFPDDDLTLAALLKSPFCGLTDDDVYALAKSRKPRTLWATLSLRTE
                     EDPRWGAASDLLAWALIEGRKRQPFEFFATLLGKVDADGQSNRAKVLTRLGGEADEAL
                     DEFLAQVMDAEQRNIRDLEALVADFAALDIIVKREMEGARREVRVMTAHGSKGLEAPI
                     VFLPETTVKRGAGGSPLLTTEDGGVLWSTGKAADCAASTRAREVRDEKEAQEALRLFY
                     VALTRARDRLVLCGRIDARTKDENVGGWYAAARAAFAHPAIAPAVHEIGDPDAPILRY
                     GPDPKTLPKETLAMVAPAPLPAWAQGLAAPEPATARYAAPSRIEDEADSRAPAPSPLA
                     KVEGLGRYRRGEIIHRLLQILPDVAPAERRAASVRLLAAERDLTDDQRLEMAAAAFGV
                     LEDDRFAAVFGPGSRAEVALAGTSPLLPQGLAISGRVDRLVVTDRQILVVDYKTNRPA
                     PDSIEHADPAYLAQMAVYVAVLREVFKDRQVEAALVWTDGPKLMPVPEKVMSDALRRI
                     G"
BASE COUNT          561 a         1334 c         1309 g          556 t
ORIGIN      
        1 ggcgacacga tcgagcagat cgccgtcgaa aaggccggaa tcatcaagcc cggcgcgccg
       61 gcggtgtcgg cccgccagca cgaagcggcc gaggacgtta tcgacaccgc cgccgccctg
      121 gccggcgtgg atctgaccct gatgggccgc gacttcgacg cctggaacga gcgcggccgc
      181 ctgctggtgc agacgcagga tcgcctgctc gacctgcccg cgccgtccct gccgggcgac
      241 catcagttcg caaatgccgg cctggccgtc gcggccctgc tggccctggc cgacccgcgc
      301 atcgacgagg ccgccatgtc cgccgggatc gccgggaccg tctggccagc gcggttccag
      361 cgcctgaccg ccgggccgct cgccgggaag gccagcgcgg cgggggccga cctgtggctt
      421 gatggcggcc acaatcctca cgccggtcag gccgtggccc gggcgctggg cgatctggcg
      481 gctcgcgacg gtcgacccgt ggcgctgatc tcgggactgc tggccaacaa ggacgccacc
      541 ggcttcttct cgccgttcgc ctcgctaaag gcccaggttt tcaccgtcac cttcgaaggc
      601 cacgccgccg ccagcgccgc ccagaccgcc gccgccgccg agctggccgg gcttcgagcc
      661 catgcctgcg acagcgtcga ggacgccttg gctaaagccc tggccctgac gccgaccccg
      721 cacgtgctga tctgcggctc gctctacttg gccggcgaag tgctggcgat gagcccggaa
      781 acctggccca cctgaaccag cgcttgggct cggcgcgccg caggggtatg gctgggctac
      841 gccgataggg cggctcggcc cgctggacct cccggcaggc gcgcccgtcc tggcaggagc
      901 atcccatggc caagggtcag aagaagtcca acaaggaagt ccgcaaaccg aaggccgaca
      961 agaaggccgc cccggcctcg gtttcgccgt tcatcgttac gtcggcgaag aagtagccgc
     1021 caaaaccaaa ccgcccaccc cgcagggcgg gatgggcggc tggatctcaa tctacgcagg
     1081 gttggctcag gccggctgct ggacgccggc ttcgttcagc cattccagga tcttctgctt
     1141 gggcatggcg ccgaccttca tcgaggtcat ctggccgccg cggaacagca tcatggtcgg
     1201 aatgccgcgc acgccatagc gcgacggcgt cgtggggctg tcctcgatat tgaccttggc
     1261 caccaccacg tggtcggcga gttcctcgga gatctgctcc agggccgggg cgatctgctt
     1321 gcaggggccg caccattcgg cccagaaatc cacgagcacc gggccgtcgg cctgcagcac
     1381 gtcgcgttcg aaggtctcgt cggtcaccgt cacggtgctc atgggtgttg ctccatctgg
     1441 gcgccgctct tgccggcgcg aaataagaaa gcccgcgagt cgtcgcgagc gcttgaagaa
     1501 gatgtaggcg ccaggaacgg tcccggcaac ggcctagccg atccgccgca gggcgtcgga
     1561 catcaccttt tctggaaccg gcatcagttt ggggccgtcc gtccacacca gagcggcctc
     1621 gacctgacgg tctttgaaca cctcgcgcag caccgccaca tagaccgcca tctgggccag
     1681 ataggccgga tcggcgtgct cgatgctgtc gggggccggg cggttggtct tgtaatcgac
     1741 caccaggatt tgccgatccg tcaccaccag ccggtcgacc cggcccgaga tcgccaggcc
     1801 ctggggcagc agcgggctgg tccccgccag cgccacctcg gctcgcgagc cgggaccgaa
     1861 cacggcggcg aagcggtcgt cttccagcac cccgaaagca gcggcggcca tttccaggcg
     1921 ctggtcatcg gtcaggtcac gctcggccgc cagcaggcgg acgctggccg cgcggcgctc
     1981 ggcgggcgcc acgtcgggca ggatctgcag cagccggtgg atgatctcgc cgcgtcgata
     2041 tcgacccagg ccctcgacct tggccagcgg cgaaggggcc ggagcccggc tatcggcctc
     2101 gtcctcgatc cgcgagggcg ccgcatagcg ggcggtggcc ggctctggcg ccgccagacc
     2161 ctgggcccag gcgggcaggg gcgccggagc gaccatcgcc agagtctcct tcggcaaggt
     2221 cttcgggtca ggcccgtaac gcaggattgg cgcgtcggga tcgccaatct catggacagc
     2281 aggcgcgatg gccggatggg cgaaggccgc gcgcgccgcc gcataccagc cgccgacatt
     2341 ctcgtccttg gtgcgcgcgt cgatgcggcc gcacaggacc agccggtcgc gcgcccgggt
     2401 cagggccaca tagaaaaggc gaagggcctc ctgcgcctcc ttctcgtcgc gcacctcgcg
     2461 ggcccgggtc gaggccgcgc agtcggcggc cttgccggtc gaccacaaca cgccgccgtc
     2521 ttccgtagtc agcagcggcg agccgcccgc gccgcgcttc accgtggtct cgggcaggaa
     2581 cacaatcggc gcctccagac ccttggagcc gtgggcggtc atcacccgca cctcgcggcg
     2641 ggcgccttcc atctcgcgct tgacgatgat gtccagggcc gcgaagtcgg cgaccagcgc
     2701 ttccaggtcg cggatgttgc gctgctcggc gtccatcacc tgggccagga actcgtccag
     2761 cgcctcgtcg gcctcgccgc ccagacgggt caggaccttg gcgcggttgg actggccgtc
     2821 ggcgtcgacc ttgcccagca gggtggcgaa gaactcgaac ggctggcgct tgcggccctc
     2881 gatcagggcc caggccagca ggtcgctggc ggcgccccag cgagggtctt cctcagtccg
     2941 cagcgacagc gtcgcccaca gggtgcgcgg cttgcgactc ttggccaggg cgtagacgtc
     3001 gtcgtcggtc aggccgcaga acgggctctt gagcagggcc gccagggtca ggtcgtcgtc
     3061 cgggaacagc acgaaccggc ccagggccag caggtcgtca aaggcgatgt gggccgaaag
     3121 cgacaggcgg tcggcgccgg ccaccggcac gccgcgccgc ttcagagccc ggatgatctc
     3181 ctcgaacagc accttgcgac ggcgcaccag gatcaggatg tcgcccgcgt gggccgcgcg
     3241 tcgggttctg gcttccttgt catggacctc gtcgccgcga tccaggatcc cagcgatctc
     3301 tgcggcgatg gcctcggcca ggcggcggtt ggcgctgtgt tcgctctcgg cgtccagcgg
     3361 ggcctcccag gcgtcgcgct cctcgccggg gcgttcgcgg gtgaggggcc acaggtcgac
     3421 gcagccgggt ccgtcggtgc ggaaggcctg gtgtggttcc agatcgccgc cgcttggcat
     3481 caatccaaga cggttttcgg ggacctcgaa cagggcgtcg acgaagctca gcacctcgcg
     3541 agtcgagcgg taggagacgc tgagcggcac cgccgagcct tcgcgcccgg cctcggcgat
     3601 gcgggcgata tagccctgcg tctcgatcag caggcgggcc gggtcggcgc cctggaacga
     3661 gtagatcgac tgcttctggt cgccgacgac aaacagggtg cggttgtcgc ggcccttgcg
     3721 ccagccggtc gcgccggccc cgacgaagaa gtcgttggtc
//
LOCUS       DAOMEO010000181         3747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2841_length_3747_cov_2.431203, whole genome shotgun sequence.
ACCESSION   DAOMEO010000181 DAOMEO010000000
VERSION     DAOMEO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_3747_cov_2.431203"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLH31_05400"
     CDS             complement(<1..200)
                     /locus_tag="PLH31_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQR88697.1"
                     /translation="MKAPPRIKQTFRIPADLSRDLADYARRRRVAQGDVVQAALASHL
                     SPDGADRLEAALARRLDRMSRQ"
     gene            complement(197..2206)
                     /locus_tag="PLH31_05405"
     CDS             complement(197..2206)
                     /locus_tag="PLH31_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HQR88698.1"
                     /translation="MSATRILWGQVLIVALIALISVWAATQWTAWRLAYQPELGAPWF
                     LFADHPIYAPPAFFWWWYFFDAYAPQIFFEGALIASAGGFASIAVAIGMSVWRAREAK
                     NVTTYGSARWATARELRAAKLLGPDGVVLGRYRRDYLRHDGPEHVLCFAPTRSGKGVG
                     LVIPTLLTWPGACIVHDIKGENLGLTAGWRARFSRVLRFDPTDADSDAYNPLLEVRRG
                     PSEVRDVQNIADILVDPEGALEKRNHWEKTSHALLVGAILHVLYAEADKTLAGVAGFL
                     SDPKRPIETTLRAMMTTPHLGDDGVHPVVASAARELLNKSENERSGVLSTAMSFLGLY
                     RDPVVARVTRRCDWRICDLVDQRRPASLYLVVPPSDISRTKPLIRLILNQVGRRLTED
                     LDAKGRRHRLLLMLDEFPALGRLDFFESALAFMAGYGIRSFLIAQSLNQIEKAYGPNN
                     AILDNCHVRVSFATNDERTAKRVSDALGTATEIRDARNYAGHRLSPWLGHLMVSRQET
                     ARALLTPGEIMQLPPTDAIILVSGLFPIRAKKARYYEDRRFKGRLLPAPVKPRKAPGP
                     QGGQDRGDDWSRLSPLAPIVSPAAAASVGGEEADADPANAGIRREPEIPPHEAVAPET
                     DRPAREFEVLEDEPDEDGMRAQTLQARMRGLARQAALDPDDGIVL"
     gene            complement(2320..>3747)
                     /locus_tag="PLH31_05410"
     CDS             complement(2320..>3747)
                     /locus_tag="PLH31_05410"
                     /inference="COORDINATES: protein motif:HMM:NF023271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HQR88699.1"
                     /translation="GAGGRLFDAQSEDVDGRAFADRCEADRHHFRFIVSPEDAGQMSD
                     LQGFTRDLMAQAERDLGTRLDWVAVTHHNTDNPHVHILVRGRADDQTDLVISRDYIGR
                     GLRAQAERLVSLELGPRSERDIAAALRREVDAERWTGLDRTLRGYADDAGGVIDLRPG
                     GAAHDDRQLHDVLVGRAQRLERLGVTEAIGPAQWTMATDAEATLRALGERGDIIKTMH
                     RALGDRGQSRGVAAFAIHGDSAPNVTGRLVDRGLQDELTGSAYVVIDGIDGRTHHVRF
                     PDLAATGDAAIGAIVEARPFTGADGRQRLALSVRSDLSLAEQITASGATWLDRHLTAR
                     DPAPVAATGFGGEVREALEQRLDRLASEGLARRRPQGPAFEKDLIATLRRRELADAAA
                     RLTKETGLPHRPSGAGDQVAGVYRQRVTLASGRFVMLDNGLGFELVPWKPALDQHLGR
                     HIAGVVQPGGTVDWTLGRKRGLGLG"
BASE COUNT          572 a         1243 c         1357 g          575 t
ORIGIN      
        1 atctggcggg acatgcgatc gagccggcgg gccagcgccg cttcaagacg atcggcgccg
       61 tcgggggaca ggtgcgaagc cagcgccgcc tggaccacat cgccttgggc gacgcggcgg
      121 cgccgcgcat agtcggcgag atcccggctg agatccgccg gtatccggaa ggtctgcttg
      181 attctcgggg gcgccttcac aggacgatcc cgtcgtcggg atcgagggcg gcctgccgcg
      241 ccaggcctcg catacgcgcc tgtagcgtct gcgctcgcat gccatcctcg tccggctcgt
      301 cctccagcac ctcgaactcg cgcgccggcc ggtccgtctc gggcgcgacg gcttcgtggg
      361 gcgggatctc aggttcgcgg cgaatgccgg cgttcgccgg gtccgcgtcg gcctcctcgc
      421 cgcccacgga tgcggcggca gcgggcgaca cgatcggcgc caaggggctg aggcggctcc
      481 agtcgtcgcc gcgatcctga ccaccctgtg gccctggcgc cttgcgcggc ttcaccggcg
      541 cgggcagcag acggcctttg aagcggcggt cctcatagta tcgggccttc ttggcgcgga
      601 tcgggaagag gccggagacc aggatgatcg catcggtcgg cggcagctgc atgatctcgc
      661 cgggggtcag cagcgcccga gccgtctcct ggcgcgacac catcagatgc ccgagccagg
      721 gcgacagccg atggcccgcg tagttgcgtg cgtcgcggat ttcggtggcg gtgcccagcg
      781 cgtcgctgac ccgcttggcg gtgcgctcgt cgttggtcgc gaagctgacc cggacatggc
      841 aattgtcgag gatcgcattg ttcgggccgt aggccttttc gatctggttg aggctctggg
      901 cgatcaggaa gctgcggatg ccgtagcccg ccatgaaggc cagggcggac tcgaagaagt
      961 cgaggcggcc gagcgccggg aattcgtcga gcatcagcag caggcggtgc cggcggcctt
     1021 tggcgtcgag gtcttcggtg agccggcggc ccacctgatt gaggatcagc cggatgagcg
     1081 gtttggtgcg cgagatgtct gagggcggca cgaccagata gaggctcgcg ggccgccgct
     1141 ggtcgaccag atcgcaaatc cgccagtcgc accgccgggt gacccgcgcg acgaccgggt
     1201 cgcgatagag ccccaggaac gacatcgcgg tcgacagcac gcccgatcgc tcgttctcgc
     1261 tcttgttcag cagctcgcgc gcggccgagg cgacgacggg gtggacgccg tcgtcgccaa
     1321 gatggggcgt ggtcatcatc gcgcgcaggg tggtctcaat gggacgcttc ggatcggaga
     1381 ggaagccggc gaccccggcc aacgtcttgt ctgcctcggc atagaggacg tgaaggatgg
     1441 ccccgaccaa cagcgcgtgg ctggtcttct cccagtggtt tcgcttttcc agggcgcctt
     1501 cgggatcgac gaggatgtcg gcgatgttct ggacgtcgcg cacctccgag gggccgcggc
     1561 ggacttccag cagaggattg taggcgtcgg aatcggcgtc ggtgggatcg aagcgcagaa
     1621 cgcggctgaa gcgggcgcgc cagcccgcgg tcaggccgag attctcgccc ttgatgtcat
     1681 gaacgatgca ggcgcccggc caggtcagga gcgtcgggat gaccagaccg acacccttgc
     1741 ccgaccgggt gggggcaaag cacagcacat gctcgggacc atcgtgtcgc agatagtccc
     1801 ggcgatagcg ccccaggacc accccgtcgg ggcccagcag cttggcggcg cgcagttcgc
     1861 gcgccgtggc ccaccgcgcc gatccatagg tcgtcacgtt cttggcttcg cgcgcgcgcc
     1921 acaccgacat gcctatggcg acggcgatcg aggcgaaacc gccggccgag gcgatcagcg
     1981 cgccttcgaa gaagatctgc ggtgcatagg cgtcgaagaa gtaccaccac cagaagaagg
     2041 cgggcggtgc atagatcgga tgatcggcga acaggaacca gggggcgccc agctccggct
     2101 gataggcgag gcgccaggcg gtccattggg ttgcggccca gaccgagatc aacgcgataa
     2161 gggcgacgat cagaacctgg ccccagagaa tgcgggtggc cgacatcgcg acactcctaa
     2221 cagggcagtg tgccgaccct gggaaaggtc atcggcgttg caaggcttcg gcccatcggg
     2281 tcgcgcggcc ggataggatg gcgtgcagat cggcggggtt cagccgaggc ccagcccgcg
     2341 cttgcgccca agcgtccagt cgacggttcc gccaggctga acgacgcctg cgatgtgccg
     2401 cccgaggtgc tggtcgagcg cgggcttcca cggcacgagc tcgaagccga ggccattgtc
     2461 gagcatcacg aaccggccgg aggcgagcgt gacccgttgg cgatagacgc ccgcgacctg
     2521 atcgccggcg cccgacgggc gatgcggcag gccggtttcc ttcgtgagcc gggccgcagc
     2581 gtctgcaagc tcacgccgcc gcagggttgc gatcagatct ttctcgaagg ccgggccctg
     2641 cggccgccgc cgggccaacc cctcgctcgc gagccggtcg aggcgttgct cgagcgcctc
     2701 gcgaacttcg ccgccgaagc cggtcgccgc cacaggcgcc gggtcacgag cggtcaggtg
     2761 acggtcgagc caggtcgctc cggaggctgt tatctgctcg gcgagcgaga ggtcggagcg
     2821 gaccgagagc gcgagacgtt gccgaccgtc cgcgccggtg aacggccggg cttcaacgat
     2881 cgcgccgatc gccgcgtcgc cggtcgccgc gaggtctggg aaacgcacgt gatgtgtccg
     2941 tccgtcgatg ccgtcgatca cgacataggc cgatccggtc agctcgtcct gcaggccgcg
     3001 gtcgaccagg cgtcccgtca cattgggcgc agagtcgccg tggatggcga aggcggcgac
     3061 gccgcgcgat tgtcctcgat ccccgagcgc ccggtgcatg gtcttgatga tgtcgccgcg
     3121 ttcgccaagc gcacgcagcg tcgcctcggc gtcggtcgcc atcgtccatt gggcggggcc
     3181 gatggcctcc gtgacgccga gccgttcgag acgctgagcg cgtccgacca ggacgtcgtg
     3241 gagctggcgg tcgtcatgcg ccgcgccgcc cggtctcaag tcgatgacgc cgccggcgtc
     3301 gtcggcgtag ccgcgcagcg tccggtcgag gccggtccag cgctccgcat ccacctcccg
     3361 gcgcagcgcc gcggcgatgt cccgctcgct tcgcggccca agctcgagcg acaccaggcg
     3421 ttcggcctgg gcgcgcaatc cgcggccgat atagtcgcgg ctgatgacca ggtccgtctg
     3481 atcgtcggcg cggccccgca cgaggatgtg gacgtgggga ttgtcggtgt tgtgatgggt
     3541 caccgccacc cagtcgaggc gcgtgccaag gtctcgttcc gcctgggcca tcagatcgcg
     3601 ggtgaagcct tgcagatcgc tcatctggcc ggcgtcctcg ggcgagacga tgaagcggaa
     3661 atgatgccga tcggcttcgc accgatcggc gaaggcgcgc ccatccacat cttcgctctg
     3721 ggcgtcgaac agccgtccgc cggcgcc
//
LOCUS       DAOMEO010000182         3745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2842_length_3745_cov_2.261247, whole genome shotgun sequence.
ACCESSION   DAOMEO010000182 DAOMEO010000000
VERSION     DAOMEO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_3745_cov_2.261247"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2880)
                     /gene="putA"
                     /locus_tag="PLH31_05415"
     CDS             complement(<1..2880)
                     /gene="putA"
                     /locus_tag="PLH31_05415"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HQR88700.1"
                     /translation="MTDWDSLDNGKYRDEAAVIADLLAAAPMSSDDRAAVRAEAEALV
                     RGARRSVRKQGVVESFLQEFSLGTREGLALMCLAEALLRTPDDDTRDKLIAEKIGSAD
                     WASHLGGSDSLFVNASTWGLMLTGKIVEPDDQAQKDLPGFIKKIAGRLGEPVIRAAVG
                     QAIRIMGEQFVLGRTIEAAIKRAASDGDICSFDMLGEGARTAADAQRYEKSYADAIET
                     VGKLSNKAGPEAGHGVSVKLSALTPRYEATQEARVWDELYPRVLRLALIAAKYDINFT
                     IDAEEADRLALSLKLLDRLCREPELGQWTGLGLAVQAYQKRCPEAVARLVALSKETGR
                     RLMVRLVKGAYWDSEIKRAQVAGRPDYPVYSTKPATDLSYLVCAKALIAAAPHLYAQF
                     ATHNAHTLAAVVRMSKAAGVKIEHQRLHGMGEALYKAADDLYDGITLRAYAPVGGHED
                     LLPYLVRRLLENGANTSFVHALLDERVPVEKVVVDPIDAVEAHPARHAKIPTPIHVYG
                     DRRQNSAGVDLSIKAERERLTAAVAALDSETLSAGPLIGGKLVAGGTPLPVVSPADHG
                     RVVGAVSEAQLPQIDQAFKLARAAQPAWDRSGGAARAKVLRAMGDALEANIDRLIAIL
                     SREAGKTLSDGIAEVREAVDFCRYYAVLAEDQFGDGEILKGPVGETNVLRMAGRGVVV
                     CISPWNFPLAIFIGQIAAALAAGNAVLAKPAEQTPLIAMEAVKLYHAAGLDPRLLALL
                     PGRGETVGAVLTAHDDLDGVAFTGGTDTAWRINQTLAQRQGPIVPFIAETGGLNGMFV
                     DTTAQREQVIDDVIVSAFGSAGQRCSALRLLFLPHDTADHIIDGLKGAMDALVLGDPA
                     LAVTDVGPVIDADAKEALEKHRVRLKREAKILHALSAPAGGTFFAPVLAEIPAADFLE
                     REVFGPVLHVVRYAPEDLEQVAGALAARRYGLTLGIHSRIESFAAD"
     gene            3315..>3745
                     /locus_tag="PLH31_05420"
     CDS             3315..>3745
                     /locus_tag="PLH31_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HQR88701.1"
                     /translation="MAISTLDDTDRRLLKVLQADGRITNAELAKQCHLSPAATFDRVR
                     RLRERGFITGYAALLDPAKVDRALLIFVEVMLERTTGEVFDDFAAAVRRAPEILECHM
                     VAGGFDYLIKARVKDMEAYRGFLGDILVKMPGVRETRTYAVL"
BASE COUNT          550 a         1200 c         1368 g          627 t
ORIGIN      
        1 gtcggccgcg aagctctcga tccgcgaatg gatgcccagc gtcaggccat agcgacgggc
       61 cgccagggcg ccggcgacct gttccagatc ttccggcgcg tagcggacca cgtgcaggac
      121 ggggccgaag acctcgcgct ccaggaagtc ggcggcgggg atctcggcca ggaccggggc
      181 gaagaacgtg ccgccggccg gggctgacag ggcgtgcagg atcttggcct cgcgcttcag
      241 acggaccctg tgcttttcca aggcctcctt ggcgtccgcg tcgatcacgg ggcccacgtc
      301 ggtgacggcc agggccggat cgcccagcac cagggcgtcc atcgcgccct tcaggccgtc
      361 gatgatgtgg tcggcggtgt cgtgcggcag gaacagcagg cgcagggccg agcagcgctg
      421 gccggcgctg ccgaaggccg agacgatgac gtcgtcgatc acctgttcgc gctgggcggt
      481 ggtgtcgacg aacatgccgt tgaggccgcc ggtctcggcg atgaacggca cgatcggacc
      541 ctgacgctgg gccagcgtct ggttgatgcg ccaggcggtg tcggtgccgc cggtgaaggc
      601 cacgccgtcc aggtcgtcat gggcggtcag caccgcgccg accgtctcgc cgcgaccggg
      661 cagcagggcc agcaggcgcg ggtccaggcc ggcggcgtga tagagcttca ccgcctccat
      721 ggcgatcagc ggggtctgtt cggcgggctt ggccagcacg gcgttgccgg cagccagcgc
      781 cgcagcgatc tggccgatga agatggccag tgggaagttc cacgggctga tgcagacgac
      841 cacgccgcga cccgccatac gcaggacgtt ggtctccccg acgggacctt tcaggatctc
      901 gccgtcgccg aactggtctt cggccagaac cgcgtagtag cggcagaagt ccacggcctc
      961 gcgcacctcg gcgatgccgt cgctgagcgt cttaccggct tcgcgcgaca ggatggcgat
     1021 caggcggtcg atattggctt ccagggcgtc gcccatggcc cgcagcacct tggcgcgcgc
     1081 ggcgccgcca gagcggtccc aggccggttg cgcggcgcgg gccagcttga aggcctggtc
     1141 gatctgggga agctgggctt ccgacaccgc gccgacgacg cgaccgtggt cggcagggct
     1201 gacgaccggc aacggcgtgc cgccggcgac cagcttgccg ccgatcagcg gaccggccga
     1261 cagggtctcg ctgtccagcg ccgcgacggc ggcggtcagg cgctcgcgct cggccttgat
     1321 cgacaggtcg acgcccgccg agttctggcg gcggtcgcca tagacatgga tcggggtcgg
     1381 gatcttggcg tggcgggccg ggtgggcctc gaccgcgtcg atggggtcaa cgaccacctt
     1441 ctccaccggc acccgctcgt cgagcagggc gtggacgaag ctggtattgg cgccgttctc
     1501 cagcaggcgg cggaccagat agggcagcag gtcttcgtga ccgcccaccg gggcgtaggc
     1561 ccgcaaggtg atgccgtcat agaggtcgtc ggcggcctta tacagagcct cgcccatgcc
     1621 gtgcaggcgc tggtgctcga tcttgacgcc ggcggccttg gacatccgca ccacggcggc
     1681 cagggtgtgg gcgttgtggg tggcgaactg ggcgtagagg tgcggcgcgg cggcgatcag
     1741 ggccttggcg cagaccaggt acgacaggtc ggtggcgggc ttggtcgaat agaccggata
     1801 gtccggacgg cctgcgacct gggcgcgctt gatctcgctg tcccaatagg cgcccttgac
     1861 cagccgcacc atcaggcgac ggccagtttc ctttgataga gctaccaggc gggcgactgc
     1921 ctcggggcag cgcttctgat aggcctggac ggcgaggcca aggccggtcc actggcccag
     1981 ttccggctcg cggcacaggc gatccagcag cttcagcgac agggccaggc ggtcggcttc
     2041 ctcggcgtcg atggtgaaat tgatgtcgta cttcgcggcg atcagggcca ggcgcaggac
     2101 gcgcggatac agctcgtccc agacgcgggc ttcctgcgtg gcctcatagc gcggcgtcag
     2161 ggccgacagc ttgaccgaca cgccgtggcc ggcttccggg ccggccttgt tcgacagctt
     2221 gccaaccgtt tcgatggcgt cggcatagga cttttcatag cgctgcgcgt cggcggcggt
     2281 gcgggcgcct tcgcccagca tgtcgaagct gcagatgtcg ccgtcgctgg cggcgcgctt
     2341 gatggcggcc tcaatggtgc ggcccagcac gaactgttcg cccatgatgc ggatggcctg
     2401 gccgacggcg gcgcggatca cgggctcgcc caggcggccg gcgatcttct tgatgaagcc
     2461 gggcaggtcc ttctgggcct ggtcgtcggg ctcgacgatc ttgccggtca gcatcaggcc
     2521 ccaggtcgag gcattgacga acaggctgtc gctgccgccc aggtgcgacg cccagtcggc
     2581 cgagccgatc ttctcggcga tcagcttgtc gcgggtgtcg tcgtcggggg tgcgtagcag
     2641 ggcctcggcc aggcacatca gggccaggcc ctcgcgggtg cccaggctga attcctgcag
     2701 gaagctctcg accacgccct gcttgcggac gctgcggcgg gcgccgcgca ctagtgcctc
     2761 agcctcggcg cggacggcgg cgcggtcatc gctgctcatc ggcgcggcgg ccagcagatc
     2821 ggcgatcacg gcggcttcgt cgcgatactt gccgttgtcg aggctgtccc agtcggtcat
     2881 gcggttcgcc cttggggatg gaatgatgat caacggtata agtacgctct agccgaatgt
     2941 gcttcgtttg taaggttgtc tggctggatg aaattcggcg acggacctca atggcgcatg
     3001 tttgctccag ccgcgcgggc cgggccgacg ccgccgtggc gctgtcagaa tggcgtgaaa
     3061 gcggagaggt gggaagggac gcgactgggg gggaggccgc gcgtcccttc cgctctcgac
     3121 gacgcctttg ggggagaagg cgtcgcgggt gtcccctctt caggcgggga cgcagtcttt
     3181 cggcgctccg ggcgccgtct ggaggaacgg cggtgcgaaa ccggctagga cgttcgatgg
     3241 tgtatagcca agtttgctga atgtgctttg tttgtaggtg agattttccg aacgatcttc
     3301 gacgaaaggt gcaaatggcc atttccactc ttgatgacac cgatcgccgg cttctgaagg
     3361 tgctgcaggc cgatggccgc atcaccaatg ccgagctggc caagcagtgc cacctgtcgc
     3421 cggccgccac cttcgaccgc gtgcgccgcc tgcgcgagcg cggcttcatc accggctacg
     3481 cggccctgct ggacccggcc aaggtcgacc gggccttgct gatcttcgtc gaggtcatgc
     3541 tggagcgcac gaccggcgag gtgttcgacg acttcgccgc cgctgtccgc cgcgcgccgg
     3601 agatcctgga atgccacatg gtggccggcg gcttcgacta cctgatcaag gctcgggtga
     3661 aggacatgga ggcctatcgg gggttcctgg gggacatcct ggtcaagatg cccggcgtgc
     3721 gcgagacgcg gacctatgcc gtgct
//
LOCUS       DAOMEO010000183         3736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2848_length_3736_cov_2.691660, whole genome shotgun sequence.
ACCESSION   DAOMEO010000183 DAOMEO010000000
VERSION     DAOMEO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2848_length_3736_cov_2.691660"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="ccoO"
                     /locus_tag="PLH31_05425"
     CDS             <1..549
                     /gene="ccoO"
                     /locus_tag="PLH31_05425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQR88702.1"
                     /translation="EGCYLCHSQMIRPLRDEVERYGHYSLAAESMYDHPFQWGSKRTG
                     PDLARVGGKYSDAWHRDHMMDPRSVVPESVMPPYKFLAEKELDYRDIADRMKAQRTVG
                     VPYTQAAIDNARKDLEAQADPFSTDAAGLRTRYGAKVVNRDFDGNPDKITEMDAAIAY
                     LQMLGTLVDFKSYKAQAPENQR"
     gene            546..713
                     /locus_tag="PLH31_05430"
     CDS             546..713
                     /locus_tag="PLH31_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HQR88703.1"
                     /translation="MSGLSYETVASFSQQAGLIYFGLIFLAGVVYALWPSKKAEFQNA
                     ARMPLDDEELT"
     gene            713..1582
                     /gene="ccoP"
                     /locus_tag="PLH31_05435"
     CDS             713..1582
                     /gene="ccoP"
                     /locus_tag="PLH31_05435"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HQR88704.1"
                     /translation="MASERETDDVTGVETTGHEWDGIRELDNPLPRWWLWTWYGCIAF
                     AVGYMILMPAWPGVTGYTKGLLGHSDRVDVSRELAALEVQRGAGAASMRKASLEQIEK
                     DPELLAYAQNVGQSVFGDNCATCHGIGGTGGAGYPNLRDDVWLWGGSLQDIQHTLLVG
                     IRADHPQTRMAQMPAFGRDELLAPAAISDMTEYVVKLSGRSADAAAVARAGPVYAAQC
                     AACHGAEGRGIQATGAPNLTDADWLYGSDRVAISGQIYAGKGGVMPAWGGRLSPETIK
                     ALSVYIHANAGGQ"
     gene            1588..3102
                     /gene="ccoG"
                     /locus_tag="PLH31_05440"
     CDS             1588..3102
                     /gene="ccoG"
                     /locus_tag="PLH31_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQR88705.1"
                     /translation="MPTLIDNTRPKPDAPSKSDTRATVSAAARAKGKGDAKGGLYKPR
                     EPIYPKLVHGRWRTVKWALLIVTLAIYYITPWIRWARPGDLPQQAVLVDFAGRRFYFF
                     DIQLWPQEVYIFTGLLVISALALFLVTALFGRLWCGYACPQTVWTDLFIVVERLFEGD
                     RNARMRLDAQPLSFNKAWRKTGKHLTWLGIAFGTGGAWIFYFHDAPTLIRTFWIGQGA
                     MTAYVSCALLTATTYVFAGMMREQVCTYMCPWPRIQGAMLDEHSLQVAYLRDRGEPRG
                     PHKKGQSWDGRGDCIDCHQCVVVCPMGIDIRDGSQLECINCGLCVDACDDILGRLGRP
                     TGLIAYDTDAAVAARACGQKAVYKPIRPRTIYYALALALVSGLTLWALIGRPKADVHV
                     IRDRNPIFVRMHDGAVRDGYTLKIANRTFEDRRFEVAFTGLEGARLSQPGRPAGSSVG
                     VLVPADEVVAVRVFVTAPPDAERPHSVPGRFVVRSGDVQAEAKTVFLSGAANPK"
     gene            3099..3593
                     /locus_tag="PLH31_05445"
     CDS             3099..3593
                     /locus_tag="PLH31_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQR88706.1"
                     /translation="MTEAVPTPTVPVKGQITGWHVLIGMVAFFGFIIAVDTLFIVKAY
                     RSFSGQVASNPYEAGLAFNRTLAQRQREAELGWNAVIERPQPGIVALRMTDKTGQPLS
                     TLSLTGTLDRPATEAGRQVLSFRPTGDGWYQATARLDGAWDLRATARNARDKFEIESR
                     LVAP"
BASE COUNT          599 a         1260 c         1218 g          659 t
ORIGIN      
        1 gagggctgct atctgtgcca ctctcagatg atccgtccgc tgcgcgacga ggtcgaacgc
       61 tatggtcact acagcctggc cgccgagagc atgtacgacc acccgttcca gtgggggtcc
      121 aagcgtacgg gtcccgacct ggcgcgtgtg ggcggcaagt attccgatgc ctggcaccgg
      181 gatcacatga tggatccgcg ctcggtggtg ccggagtccg tgatgccgcc ctacaagttc
      241 ctggccgaga aggagctgga ttaccgcgac atcgccgatc gcatgaaggc ccagcgcacg
      301 gtcggggtgc cttataccca ggcggcgatc gacaacgccc gcaaggatct cgaggcgcag
      361 gccgatccct tctcgaccga cgcggcgggc cttcggacgc gctacggcgc caaggttgtc
      421 aaccgcgact tcgacggcaa tcccgacaag atcaccgaga tggacgccgc catcgcctat
      481 ctccagatgc tcggcactct ggttgatttc aaatcctaca aggcccaggc gccggagaat
      541 caacgatgag cgggctctcc tatgaaaccg tggcgagctt ttcccagcag gcggggctga
      601 tctatttcgg cctgatcttc ctcgccggcg tcgtctacgc cctgtggccg tccaagaaag
      661 ccgaattcca gaacgccgcg cgaatgccgc tggatgacga ggagctgacc tgatggcgtc
      721 cgaacgcgaa accgacgacg tcaccggcgt cgaaaccacc ggacacgaat gggacggcat
      781 ccgcgagctg gacaatcccc tgccgcgctg gtggctgtgg acctggtacg gctgcatcgc
      841 ctttgcggtg ggctacatga tcctgatgcc ggcctggccg ggtgtgaccg gctataccaa
      901 gggcctgctg ggtcactccg accgtgtcga cgtctcccgt gagctggcgg ccctggaggt
      961 ccagaggggg gctggcgccg cctcgatgcg caaggccagc cttgagcaga tcgaaaagga
     1021 tcccgagttg ctggcctatg cccagaacgt cggccagtcg gtctttggcg acaactgcgc
     1081 aacctgccat ggcatcggcg gcaccggcgg cgcgggctat cccaacctgc gcgacgacgt
     1141 ctggctgtgg ggcggttccc tgcaggatat ccagcacacc ctgctggtcg gcattcgcgc
     1201 tgatcatccc cagacgcgca tggcgcagat gcccgccttc ggccgtgacg aactgctggc
     1261 gccagcagcg atcagcgaca tgaccgaata tgtggtcaag ctttcgggac gctccgccga
     1321 cgccgcagcc gtggcgcgcg ccggccccgt ctatgctgcc cagtgcgccg cgtgccacgg
     1381 tgcggaaggc cgcggcatcc aggccacggg tgcgccgaac cttacggatg ctgactggct
     1441 ctatggctcc gaccgtgtgg cgatcagcgg ccagatctat gccggcaagg gcggcgtcat
     1501 gccggcctgg ggcggtcgtc tcagccccga gaccatcaag gccctgtcgg tgtacatcca
     1561 cgccaacgcg ggcggtcagt aggggccatg cccaccctca tcgacaacac ccgtcccaag
     1621 cccgacgctc cctccaagtc cgacacccgg gcgacggtct cggccgccgc tcgggccaag
     1681 ggcaagggcg acgccaaggg cggcctctac aagccgcgtg agccgatcta tcccaagctg
     1741 gttcacggcc gctggcgcac cgtgaagtgg gcgctgctga tcgtcaccct ggcgatctac
     1801 tacatcacgc cctggatccg ttgggcccga cctggcgacc tgccgcagca ggcggtgctg
     1861 gtggatttcg ccggtcgccg cttctacttc ttcgacatcc agctgtggcc gcaggaagtc
     1921 tatatcttca ccggcctgct ggtgatctcg gctctggcgc tgttcctggt cacagccctg
     1981 tttggccggt tgtggtgcgg ctatgcctgt ccgcagaccg tctggaccga cctctttatc
     2041 gtggtcgagc gcctgttcga aggcgaccgc aacgcccgca tgcgtctgga cgcccagccc
     2101 ctgtcgttca acaaggcctg gcgcaagacc ggcaaacacc tgacctggct gggcatcgcc
     2161 tttggcacgg gcggcgcctg gatcttctat ttccacgacg cgccgaccct gatccgcacc
     2221 ttctggatcg gacagggggc gatgacggcc tatgtctcct gcgccctgct gacagcgacc
     2281 acctatgtct tcgccggcat gatgcgcgag caggtctgca cctatatgtg cccctggccc
     2341 cggatccagg gcgccatgct ggacgagcat tcgctgcagg tggcctatct gcgcgaccgc
     2401 ggcgagccac gcggccccca caagaagggt cagtcctggg acgggcgggg cgactgcatc
     2461 gactgtcacc agtgcgtcgt cgtgtgcccg atgggcatcg acatccgcga tggcagccag
     2521 ctggagtgca ttaattgcgg cctctgcgtc gacgcctgcg atgacatcct gggtcggctg
     2581 ggtcgtccca ccggcctgat cgcctatgac accgacgcgg ccgtcgccgc gcgcgcctgc
     2641 ggccagaagg cagtctacaa gcccatccga cctcgcacga tctactacgc cctggcgctg
     2701 gcgctggttt ccggcctgac cctctgggcg ctgatcggcc ggccgaaggc tgatgtccat
     2761 gtcattcgcg accgcaaccc gatctttgtt cgcatgcacg atggcgcggt gcgtgacggc
     2821 tacaccctga agatcgccaa ccgcaccttc gaggatcgcc gttttgaggt cgccttcacg
     2881 gggctcgagg gcgcccggct cagccagccg gggcggcccg ctggctcgag cgtcggggtg
     2941 ctggtgcccg ctgacgaggt ggtggccgtg cgggtgtttg tcaccgcgcc gccggatgcg
     3001 gagcgtccgc acagcgtgcc aggccgcttc gtcgttcgct cgggcgacgt ccaggccgaa
     3061 gccaagaccg tgtttctttc cggagccgcg aacccgaaat gaccgaggct gtccccacgc
     3121 cgaccgttcc cgtgaagggt cagatcaccg gctggcatgt gctgatcggg atggtggcgt
     3181 tctttggctt catcatcgcc gtcgacaccc tgttcatcgt taaggcctat cgcagcttct
     3241 cgggccaggt ggcgtccaat ccgtatgagg cgggtctggc cttcaaccgc acgctggccc
     3301 agcgccagcg cgaggcggaa ctgggctgga acgccgtgat cgagcgtccc cagcccggga
     3361 tcgtggcctt gagaatgacg gacaagaccg gtcagccgct gtcgacgctg tcgctgaccg
     3421 gaacgctcga ccgcccggct acggaagccg ggcgtcaggt cctgagtttc aggccgaccg
     3481 gtgatggctg gtatcaggcc acggcccggc tcgacggcgc ctgggacctt cgcgcgacgg
     3541 ctcgcaatgc tcgcgacaag ttcgagatcg aaagccgtct ggtggcgcca tgacccgggc
     3601 gctggcgatt tcgcgtgatc ttgaggcctt tgtccgccgc gatgcgacgg gaacagggcg
     3661 tctggagctg ctggtcaccg gcgcccgctg cgccgcctgc atcggcaaga tcgaaagagc
     3721 cgtgagcgat ctgccg
//
LOCUS       DAOMEO010000184         3729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2856_length_3729_cov_2.612684, whole genome shotgun sequence.
ACCESSION   DAOMEO010000184 DAOMEO010000000
VERSION     DAOMEO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_3729_cov_2.612684"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLH31_05450"
     CDS             complement(<1..200)
                     /locus_tag="PLH31_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQR88707.1"
                     /translation="MARTLEASGTYRVLRRLSPHVTVAPPSGQLTRQGLVVDVETTGL
                     DAARHEIIELAMVPFTYGLDGQ"
     gene            complement(239..799)
                     /locus_tag="PLH31_05455"
     CDS             complement(239..799)
                     /locus_tag="PLH31_05455"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR88708.1"
                     /translation="MTHKPEIYISVDVETAGPIPGEYSLLSIGACDVDDESRSFSIEL
                     KPINTNADPKALEVTGLSLDELAIRGETPEAAMAAFGAWARSLAGEDRSLVFVGFNAP
                     FDWSFVNYYFHRFAGGNPFGFTALDIKAMYMGATGSTWADTRSSQVTKHLVPESRGDH
                     DALHDAKHQAELFRLVRALTLKSELR"
     gene            complement(796..>1416)
                     /locus_tag="PLH31_05460"
     CDS             complement(796..>1416)
                     /locus_tag="PLH31_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88709.1"
                     /translation="GARRLYNFGLTSHQTRRLFQIADDGDAHHWLNRIRFPARQATSD
                     RVNDLIELLESLEPPRAFAVRMLNPDLPEYAAVQNFFDTVVQPVIEGELGYRLVVVDG
                     RQAYDHARIDEEIFAKLHRSSVVLADITGARPNCFLELGYALGRGLPTMVTVQQGASL
                     PFDITTLSGLHWSTEGSAEDRRRAFREHWRAIRNRPPLVPAEPLIS"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1427..1979)
                     /locus_tag="PLH31_05465"
     CDS             complement(<1427..1979)
                     /locus_tag="PLH31_05465"
                     /inference="COORDINATES: protein motif:HMM:NF036729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88710.1"
                     /translation="MARSPLHGRRIHIAGSIVEDVAVATGEDVKLAREFVVELVKALV
                     RRGANFVVPVDAEPKRKADGLPICFDWLIWQTLRDNLIHRPAGVPGPMAIAVQHHKSE
                     DQIPAEFVDLWDDLRGNLALVQIENAAHWNMASKRMEAQARSGDILVALGGTEGVLFL
                     ANLYHDGGKPIVPLNLPLCPESTG"
     gene            complement(1979..2875)
                     /locus_tag="PLH31_05470"
     CDS             complement(1979..2875)
                     /locus_tag="PLH31_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88711.1"
                     /translation="MSQTSIFDSETPLPDEGLARREKTLLGFGTRYARVHDQLRLLLN
                     VGQLASWNRQHHGGRLALCDLVAEQYPLVIFHGDVGTGKTAMAECMANRLVAEARSED
                     SILFKLSNRVRGSGMVGEMGTLIAEAFKKVVASAGKHRRAILIIDEGDSLAAARSQQH
                     SHHEDKVAVNTLIQCVDDLRQYGGRIVVFLCTNRLSVVDAALQRRAAIMEEFRRPSDE
                     ERRQLLSMDLDALSLSPAQIGQLVTATGPRSGLPGWTYSDIRTRLYPAALAKAYPDSA
                     LRFEHLQTVATTLKASPVMEDR"
     gene            complement(2872..3372)
                     /locus_tag="PLH31_05475"
     CDS             complement(2872..3372)
                     /locus_tag="PLH31_05475"
                     /inference="COORDINATES: protein motif:HMM:NF037203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88712.1"
                     /translation="MTQTRTASATATYTVLDIEKVVRRVKADLAMIADSTGGWTPEKA
                     AHYAHDVEALAKAGYLDYVDVTLLSNGVEVMATRFDVDTDAGGLTSSRPGGVLWPKVT
                     SPHLRIVLGYSSIYTATAKAAFKGQLKINWSPTDADTSHSGLSSAGGRDYASNTYGMQ
                     RRDWAA"
     gene            complement(3376..>3729)
                     /locus_tag="PLH31_05480"
     CDS             complement(3376..>3729)
                     /locus_tag="PLH31_05480"
                     /inference="COORDINATES: protein motif:HMM:NF020810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88713.1"
                     /translation="KSFVIELIMAHVLAEQGADGSIEQRFRNFLLYIAQSGLKDEIRF
                     PENVAPFTVFTDPVVILDPVYSRNNVASRISESERLEIVAAATEAWETANFASADNDN
                     DVWKEIFGPRFKTEE"
BASE COUNT          660 a         1167 c         1195 g          697 t
ORIGIN      
        1 atctggccat cgaggccata ggtgaacggc accatggcca gttcgataat ctcgtggcgg
       61 gcagcatcca ggcccgtcgt ctccacatcg accaccaatc cctgacgggt cagctggcca
      121 gaagggggag cgaccgtgac atgcggactt aaccgccgca gaactcggta ggtcccgcta
      181 gcctccagcg ttcgcgccat gccctcaaga tcttccaaac gtcccactct gctatcgatt
      241 agcgtagctc actctttaaa gtgagcgccc gcaccagcct gaataactca gcctgatgct
      301 tggcgtcatg gagcgcgtcg tgatcgccgc ggctttccgg gacgagatgc tttgtcacct
      361 ggcttgatcg cgtgtctgcc cacgtgctgc cagttgcccc catgtacatc gccttgatgt
      421 cgagcgcggt gaagccgaac gggtttccgc ccgcgaaccg gtgaaaatag taattcacaa
      481 aggaccagtc gaagggcgcg ttgaacccca cgaacacgag ggagcgatct tcaccggcga
      541 gcgaacgggc ccaggcgccg aacgcggcca tggccgcctc gggcgtttcg cctctgatcg
      601 ccagctcatc gagggataga cctgtcacct ccagcgcctt ggggtcggcg ttggtgttga
      661 tgggcttcag ctcgatgctg aaggacctgg attcatcgtc cacgtcgcag gcgccgatcg
      721 acaacaggct gtactcgccc gggatagggc cggcggtttc gacatcaacc gaaatgtaaa
      781 tttctggctt gtgggtcatg agatcagcgg ctccgcaggg acgagcggcg gccggttgcg
      841 gatcgccctc cagtgttcgc gaaacgcgcg gcgacgatct tcggcggagc cctccgtcga
      901 ccagtgcagg ccagacagtg tcgtaatatc gaatggcaag ctggcgccct gctggacggt
      961 caccattgtc ggcaggccgc gacccagggc gtagccgagc tctagaaagc agttaggccg
     1021 ggcaccggtg atgtcagcca gcacgacgct gctccggtgt agcttggcga aaatctcttc
     1081 gtcgatgcgc gcgtgatcat atgcctggcg accatcgacg acgaccagcc gataccccag
     1141 ttcgccctcg atcaccggct gcacgaccgt gtcgaagaag ttctggaccg cggcgtattc
     1201 cggcagatcg gggttcagca tccgcacggc gaacgcccga ggcggctcca gggattcgag
     1261 cagttcgatc aggtcgttga cgcggtccga agtcgcctgt cgggccggaa agcgaatgcg
     1321 gttcagccaa tggtgagcgt caccatcgtc cgcaatctgg aaaagtcgcc gggtctggtg
     1381 gcttgtgagg ccgaagttat agaggcgccg tgcgccnnnn nnnnnncgcc cgtgctttcc
     1441 ggacaaaggg gcaagttcag cgggacaatc ggcttgccgc catcgtggta caggttggcg
     1501 aggaagagga cgccttccgt tccgcccagg gcgacaagga tgtcgcccga ccgggcctgc
     1561 gcctccatgc gcttgctcgc catgttccag tgggccgcgt tctcgatctg aacgagggcg
     1621 agattgcccc gaaggtcgtc ccacagatcg acaaattccg ccgggatctg gtcctcgctc
     1681 ttgtggtgct ggaccgcgat cgccatcggc ccggggacac cggcggggcg atggatgagg
     1741 ttgtcccgca gggtctgcca aatgagccag tcgaaacaga tcggcaggcc atcggccttt
     1801 ctcttgggct cggcgtccac ggggacgacg aaattggcgc cgcgcctgac cagagccttc
     1861 accagctcaa cgacaaactc acgcgccagc ttgacgtctt cccctgtggc gacagccacg
     1921 tcctcgacaa tgctgccggc gatgtggatg cgccggccgt gaagcgggga acgggccatc
     1981 agcgatcttc catcaccggc gaggccttga gcgtggtcgc caccgtctgc aggtgctcga
     2041 accgaagggc gctgtcggga taggccttgg ccagggccgc gggatagagg cgcgtccgga
     2101 tatcggaata ggtccagccc ggcaggccag accgtggccc cgtcgctgtg accagttggc
     2161 cgatctgggc ggggctgaga gacaaggcgt ccaggtccat cgacaggagc tggcggcgct
     2221 cttcatccga tggcctgcgg aattcctcca tgatcgcggc gcggcgctgc aaggcagcgt
     2281 cgacgaccga caaacggttg gtgcacagaa agaccacgat acgaccgccg tattggcgaa
     2341 ggtcatcgac gcattggatc agcgtgttta ccgccacctt atcctcgtgg tggctgtgct
     2401 gctgcgaccg cgcggccgcc aggctgtccc cctcgtcgat gatcaggatg gctcggcggt
     2461 gcttgccggc cgacgccacc accttcttga acgcctcagc gatcagggtg cccatctcgc
     2521 cgaccatgcc gctgccgcgg acccggttgc tcagcttgaa gaggatcgag tcttcgcttc
     2581 gcgcttcggc cacaagccga ttggccatac actccgccat ggccgtcttg ccggtgccga
     2641 cgtcgccatg gaagatcacc agcgggtatt gctcggcgac aagatcgcaa agggccagcc
     2701 ggccgccgtg gtgttgccgg ttccagctgg ccagttggcc gacattcagt agcagccgca
     2761 gctgatcatg gacgcgggcg tagcgcgtgc cgaaacccag caaggtcttc tcccggcgcg
     2821 ccagcccttc gtccggcagg ggcgtttcac tgtcaaagat cgacgtctgg ctcacgcggc
     2881 ccagtccctt cgctgcattc cgtaggtgtt gctcgcgtaa tcgcggccgc cggccgagct
     2941 caggccgcta tggctggtgt cagcgtcggt cggcgaccag ttgattttca gctggccctt
     3001 gaaggccgcc ttcgccgtgg cggtgtagat gctcgaataa ccgaggacaa ttcgcaggtg
     3061 gggcgacgtc accttcggcc agaggacccc tccgggccga ctggacgtca atccgccagc
     3121 gtcggtatcg acatcaaagc gggtggccat cacctcgacg ccgttgctca gcagcgtgac
     3181 atcgacgtag tcgaggtatc cggccttcgc cagcgcctcg acatcgtgtg cgtagtgagc
     3241 ggccttctcc ggcgtccacc cgcccgtgct atcggcgatc atggccaggt cggctttcac
     3301 gcggcgaacc accttctcaa tgtcgagaac ggtgtaggtc gcggtggctg aggcggtgcg
     3361 ggtctgggtc atcgcctatt cctccgtttt gaagcgaggc ccgaagattt ccttccagac
     3421 gtcgttgtcg ttgtcggcag aggcgaagtt cgccgtctcc caagcctcag tcgccgccgc
     3481 cacgatctca aggcgttcag attcgctgat ccggctagcg acgttgttgc ggctgtagac
     3541 ggggtcgagg atcacaacgg gatcggtgaa gacggtgaag ggcgccacgt tttcggggaa
     3601 ccggatctcg tccttgagcc ccgactgggc gatgtagagc aggaagttcc ggaaccgttg
     3661 ctcgatcgag ccgtcggcgc cctgttcggc cagaacgtgg gccatgatca attcgatgac
     3721 gaaggattt
//
LOCUS       DAOMEO010000185         3724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2860_length_3724_cov_2.371763, whole genome shotgun sequence.
ACCESSION   DAOMEO010000185 DAOMEO010000000
VERSION     DAOMEO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_3724_cov_2.371763"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /locus_tag="PLH31_05485"
     CDS             complement(<1..69)
                     /locus_tag="PLH31_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQR88714.1"
                     /translation="MQAIAFYLLAFVTLAAGILVVSA"
     gene            complement(147..>250)
                     /locus_tag="PLH31_05490"
     CDS             complement(147..>250)
                     /locus_tag="PLH31_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HQR88715.1"
                     /translation="REELYYDKERLLDNGDRWERLIAKNLELDAPYR"
     assembly_gap    251..260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(261..637)
                     /gene="nuoI"
                     /locus_tag="PLH31_05495"
                     /pseudo
     CDS             complement(261..637)
                     /gene="nuoI"
                     /locus_tag="PLH31_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517393.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            complement(643..882)
                     /locus_tag="PLH31_05500"
     CDS             complement(643..882)
                     /locus_tag="PLH31_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88716.1"
                     /translation="MNRQTAFALLGLSVASLAACSATTASDKSYSLGRGVVNYDELRR
                     AGDKCKVEGGALQAKQDGGDPAQLSNYTCVIPKGD"
     gene            complement(882..1949)
                     /gene="nuoH"
                     /locus_tag="PLH31_05505"
     CDS             complement(882..1949)
                     /gene="nuoH"
                     /locus_tag="PLH31_05505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQR88717.1"
                     /translation="MSEFFSNPAVTWTLLTTGGILLVVIWVLLSLAFLLLADRKIWAG
                     VQMRKGPNVVGPFGLLQSFADFFKFVLKEIIIPAGADKVVFILAPLLSLVLAFVGWAA
                     IPFAPGWVVSNLNVGILYLLAMSSLGVYGIIMGGWASNSKYPFLGALRSAAQMVSYEV
                     SIGLIIITVILLAGSMNLTTIVEAQSGWFWNWNVFGGGLRNLVLLPVMVIAMGMFYIS
                     ALAETNRPPFDLPEAESELVAGYQVEYSSTPYLLFMVAEYSNIVLMCAMISVLFFGGW
                     NPGFPTDFLVSWGWWGNFFLALVFFAKICFWFFMIAMAKAIVPRYRYDQLMRLGWKVF
                     LPTSLVLVVLVAAWRVFGPGA"
     gene            complement(1956..>3724)
                     /gene="nuoG"
                     /locus_tag="PLH31_05510"
     CDS             complement(1956..>3724)
                     /gene="nuoG"
                     /locus_tag="PLH31_05510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HQR88718.1"
                     /translation="CPICDQGGECDLQDQAMGYGRDDSRYEENKRAVEEKAMGPLIKT
                     VMTRCIQCTRCVRFITEVAGSPEIGLISRGEDVEIMTYLGAAVTSELSANVIDLCPVG
                     ALTSKPYAFEARPWELKKTESIDVMDALGSAIRVDSRAAAVLRVLPRVNEDVNEEWIS
                     DKTRYAVDGLQRQRLDRPYVRIGGRLQPATWGQAFAAVAEKIKATAPERIGVIAGDLQ
                     DAESLKATKDLFTALGVKNLDARQDGVALGDGPRESWLFNSTIAGIENADVILLVGTN
                     PRVEAPVLNTRLRKQWLAGKVRIGLIGEQGDLTYEYDYLGAGTKSLSGLAKSKSDFVT
                     ALKNAERPAIIIGQGALTRADSAAVLKAAAGVAKTFNVVKSGWNGWNVLHTAASRVAG
                     LDMGFVPGEGGLTAGQMLKPGALDVLFLLGADECETAASDAFKIYLGTHGDAGAHKAD
                     VILPGAAYTEKNGLYVNTEGRVQMGERAVFPKGEAKEDWSILRALSEILGHKLPYDSL
                     DQLRARLFADHPTFGQIDFAPGTIPTAFDVAALGGAGELADTPFENAIKAFHLTNPIA
                     RASVTMAECAAVTSGAAKIAAE"
BASE COUNT          675 a         1202 c         1214 g          623 t
ORIGIN      
        1 cgccgagacg acgaggatac ccgccgccag cgtcacaaag gccagcaaat agaaagcgat
       61 cgcctgcaag gctgctcccc tgaaatcaac attcacgacg acggcgccga ccggcgcatc
      121 gcccccttcg gattcgcgtc ccgtccttaa cggtaaggcg cgtccaactc cagattcttc
      181 gcgatcagac gttcccagcg gtcgccgtta tcgagaagcc gttccttgtc gtaatagagt
      241 tcttcgcggg nnnnnnnnnn aactcggtgt tcggcccttc cacgatggcg tccaccgggc
      301 aggcctcctg gcacaggccg cagtagatgc acttgaccat gtcgatgtcg tagcgggtcg
      361 tacggcggct gccgtcctcg cgcggttcgg cttcgatggt gatggcctgg gcggggcaga
      421 tggcctcgca cagcttgcac gcgatgcagc gctcctcgcc cgacggatag cgacgcaggg
      481 cgtgctcgcc ccggaagcgc ggcgactgcg ggttgcgctc gttgggatag ataacggtct
      541 tcttcggcgc gatcatgtat ttcatcgcga ggccaaaggc cccggcgaag tcgagaaggg
      601 ccgcgccctt aatcgcctgg gaaatgcgtt gcatcatggc cgtcaatcac ccttcggaat
      661 gacgcaggtg tagtttgaaa gctgggcggg atctcccccg tcctgcttgg cttgcagagc
      721 tccgccctcg accttgcact tgtcgccggc ccggcggagt tcgtcatagt tcacaacgcc
      781 acgccccagc gaataggact tatccgacgc cgtcgtcgcc gaacaggcgg cgagcgaggc
      841 gacgctcagg cccaacaggg cgaaggcggt ttggcggttc atcacgcgcc cggaccaaac
      901 acgcgccagg ctgcgaccag aacgaccaga accagcgacg tcggcaggaa caccttccag
      961 cccagccgca tcagctggtc gtagcggtag cgcggcacga tggctttcgc catggcgatc
     1021 atgaagaacc agaagcagat cttggcgaag aacaccaagg ccaggaagaa gttgccccac
     1081 cagccccagc tcaccaggaa atcggtcggg aaaccggggt tccaaccgcc gaagaacagg
     1141 acgctgatca tcgcgcacat caagacgatg ttggaatatt cagcgaccat gaacagcagg
     1201 tacggggtcg agctgtactc gacctgatag ccggccacga gttcggattc ggcttcggga
     1261 aggtcgaacg gagggcggtt ggtctcggcc agggccgaga tgtagaacat gcccatggcg
     1321 atcaccatca cgggcagcag gaccaggttc ctgaggccgc cgccaaagac gttccagttc
     1381 cagaaccagc cggactgggc ttccacgatg gtcgtcaggt tcatcgaacc ggccagcagg
     1441 atcaccgtga tgatgatcag gccgatcgag acttcgtacg aaaccatctg ggccgccgag
     1501 cgcagggcgc ccaggaacgg atacttcgag ttcgaagccc agccgcccat gatgatgcca
     1561 tagacgccca gcgaactcat cgccagcagg tagaggatgc cgacgttcag gttggagacg
     1621 acccagcccg gagcgaacgg aatggccgcc cagccgacaa aggccagaac caggctgagc
     1681 aacggcgcca ggatgaagac caccttgtcg gcgccggccg ggatgatgat ttccttcagc
     1741 acgaacttga agaagtcggc gaaggactgc agcaggccga acggacccac cacgttgggg
     1801 cccttgcgca tctgcacgcc ggcccagatc ttgcggtcgg ccagcagcag gaaggccaga
     1861 ctcagcagca cccagatcac gaccagcagg atcccgccgg tcgtcagcag ggtccaggtc
     1921 acggcggggt tggagaagaa ctcgctcatg tcggcctact ccgccgcgat cttggctgcg
     1981 cccgaggtca cggccgcgca ttcggccatg gtcacgctgg cgcgcgcgat gggattggtc
     2041 aggtggaagg ccttgatggc gttctcgaac ggcgtatcgg ccagctcgcc ggcgccgccg
     2101 agggctgcga cgtcaaacgc ggtcgggatc gtgcccggcg cgaagtcgat ctggccgaaa
     2161 gtagggtgat cggcgaacag cctggcgcgc agttggtcca ggctgtcata gggcagcttg
     2221 tggccgagga tttccgacag agcccgcagg atcgaccagt cttccttggc ctcgcccttc
     2281 gggaacaccg cccgttcgcc catctggacg cggccttcgg tgttcacata gaggccgttc
     2341 ttctcggtat aggccgcgcc cggcaggatg acgtcggcct tgtgcgcgcc ggcgtcgccg
     2401 tgggtgccca gatagatctt gaaggcgtcc gaggcggcag tctcgcactc gtcggcgccc
     2461 agcaggaaca gcacgtccag cgcgcccggc ttcagcatct ggccagcggt caggccgccc
     2521 tcgcccggga cgaagcccat gtcgagaccc gcgacgcgcg aggcggcggt gtgcaggacg
     2581 ttccagccgt tccagccgga cttcacgacg ttgaacgtct tggccacgcc agcggcggcc
     2641 ttgagcacgg ccgcgctgtc ggcgcgggtc agagcgccct ggccaatgat gatggcgggg
     2701 cgctcggcgt tcttcagagc ggtgacgaag tcggacttgc tcttggccag acccgacagg
     2761 ctcttcgtgc ccgcgccgag atagtcgtat tcgtaggtca ggtcgccctg ctcgccgatc
     2821 aggccgatgc ggaccttgcc agccagccac tgcttgcgca gacgggtgtt gagcaccggc
     2881 gcttcgacgc gcgggttcgt gccaaccagc aggatgacgt cggcgttttc gatgccagcg
     2941 atcgtcgaat tgaacagcca gctctcgcgc ggaccgtcgc ccagcgccac gccgtcctgg
     3001 cgggcgtcga ggttcttcac gcccagggcg gtgaagaggt ccttggtggc cttcaggctt
     3061 tcggcgtcct gcaggtcgcc ggcgatgacg ccgatgcgct ctggcgcggt cgccttgatc
     3121 ttctcggcca cggccgcgaa ggcctgaccc caggtcgcgg gctgcaggcg gccgccgatg
     3181 cggacatagg gacggtccag acgctggcgc tgcaggccat caacggcgta gcgcgtcttg
     3241 tcggagatcc actcctcgtt gacgtcctcg ttgacgcgcg gcagcacgcg gagcaccgcg
     3301 gcggcgcggc tgtcgacgcg gatcgccgag cctagcgcgt ccatcacgtc gatgctctcg
     3361 gtcttcttca gctcccaggg ccgcgcctcg aaggcgtagg gcttggaggt cagggcgccg
     3421 accgggcaaa ggtcgatcac gttggcgctc agttccgacg tcaccgccgc gccgagatag
     3481 gtcatgattt ccacgtcttc gccgcgcgag atcagaccga tttccggcga gccggcgact
     3541 tcagtgatga agcgaacgca gcgcgtgcac tggatgcagc gcgtcatcac ggtcttgatg
     3601 agggggccca tggccttctc ttcaaccgcg cgcttgttct cttcgtagcg gctgtcgtcg
     3661 cggccatagc ccatggcctg gtcctgcaga tcgcactcgc cgccctgatc gcagatcggg
     3721 caat
//
LOCUS       DAOMEO010000186         3699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2878_length_3699_cov_2.442645, whole genome shotgun sequence.
ACCESSION   DAOMEO010000186 DAOMEO010000000
VERSION     DAOMEO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_3699_cov_2.442645"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="ahpF"
                     /locus_tag="PLH31_05515"
     CDS             complement(<1..1081)
                     /gene="ahpF"
                     /locus_tag="PLH31_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HQR88719.1"
                     /translation="MLDDGLKTQLKAYLQNLRQPIELVASLGDGASSRDMLALLEDVA
                     GTSDKVSLSLTGGDARKPSFAITRAAGDADVRFAGLPLGHEFTSLVLALLHVGGHPPK
                     FDAEALDRVRALDGDYHFETYFSQSCQNCPDVVQALNTMSALNPRITHVAIDGALFQE
                     EVEARQIMAVPTILLNGQPFGQGRMDLEQILAKLDTGAEARMAEKIKTKDAFDVLVIG
                     GGPAGAAAAIYAARKGIRTGVAAERFGGQVLDTMAIENFISVPHTEGPKLVSGLEQHV
                     KDYDVDIMNLQKAVGLVPAKTPGGLIEIKLENGASLKSRSVILSTGARWRNVNVPGET
                     EYKNKGVAYCPHCDGPLFKGKRVAVI"
     gene            complement(1220..>1457)
                     /locus_tag="PLH31_05520"
     CDS             complement(1220..>1457)
                     /locus_tag="PLH31_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR88720.1"
                     /translation="VMREGVGLADRGTFLIDPEGVIQFMEITAEGIGRNAAELIRKIK
                     AAQYVAAHPGEVCPAKWEEGEKTLAPSLDLVGKI"
     assembly_gap    1458..1467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1468..1798)
                     /locus_tag="PLH31_05525"
     CDS             complement(<1468..1798)
                     /locus_tag="PLH31_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQR88721.1"
                     /translation="MALINTTIKPFTAQAYKAGKFIEVSDADLKGKWSVVFFYPADFT
                     FVCPTELEDLADNYPELQKMGVEVYSVSTDTHFSHKAWHDSSPAIGKIQYTMIGDPSG
                     LITNNFDV"
     gene            1923..2834
                     /locus_tag="PLH31_05530"
     CDS             1923..2834
                     /locus_tag="PLH31_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HQR88722.1"
                     /translation="MLPTLRQLQYLKLLSEHGSFSRAAESAHVTQPTLSAGIQELEKI
                     LGAAVVDRARSGVILTAAGQEAVRRAQDILTRTEDLVQAARGAGLPLAGRFRLGVIPT
                     VAPYLLPRALPLLRDRFPKLRLFLREDLTQRLIASLKAGALDAALIALPYDMSGLDWA
                     HVEDDELLAAAPANHPMAASARVDPDSLVGEDLILLEDGHCLRDHALAACGLEAPSRH
                     ANGGFGDEESFAATSLPTLVQMIGSGLGISFLPAMAVQAGLTENAAVTVRPLDHAHSS
                     REIVVAWRAGSTRAAEGRLLAETLQAV"
     gene            2959..3549
                     /locus_tag="PLH31_05535"
     CDS             2959..3549
                     /locus_tag="PLH31_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQR88723.1"
                     /translation="MFGRHHHHHGRRDGGHWGAHHSRHPFGVHGHDHHGRGPGRGGGR
                     MGRFFDHGDLRLVVLKLIAEQPSHGYELIKAVETAAGGAYTPSPGVIYPTLTLLEELG
                     YITVIEAGGGKKLHTITPEGEAFLEANAQPVKSLFERMAEAAARSAAFSPQILRAREN
                     LKTALRLKLTAGQLTAEQVDAIAKALDDAAGVIERI"
     gene            3561..>3699
                     /locus_tag="PLH31_05540"
     CDS             3561..>3699
                     /locus_tag="PLH31_05540"
                     /inference="COORDINATES: protein motif:HMM:NF021496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HQR88724.1"
                     /translation="MQSHARLTTDKAARYMTQLAKHWSHKFAVTFDETTAHIPLPIGE
                     CR"
BASE COUNT          606 a         1223 c         1236 g          624 t
ORIGIN      
        1 cgatcaccgc cacgcgcttg cccttgaaca gggggccgtc gcagtgcggg caataggcca
       61 cgcccttatt cttgtactcg gtctcgccgg gcacgttgac gttccgccag cgggcgccgg
      121 tcgacaggat cacgctgcgc gacttcaggc tggcgccgtt ctccagcttg atctcgatca
      181 ggccgccggg agtcttggcc ggaaccaggc ccacggcctt ctgcagattc atgatgtcga
      241 cgtcatagtc cttgacgtgc tgctccaggc ccgagaccag cttgggaccc tcggtgtggg
      301 gcaccgagat gaagttctcg atggccatgg tgtccagcac ctgaccgccg aagcgctcgg
      361 cggcgacgcc ggtgcgaatg cccttgcggg cggcatagat cgccgccgcg gcgccggccg
      421 ggccgccgcc gataacgaga acgtcgaagg cgtccttggt cttgatcttc tcggccatcc
      481 gcgcctcggc gccggtatcc agcttggcca ggatctgttc caggtccatg cggccctggc
      541 cgaagggctg gccgttcagc aggatggtgg gcaccgccat gatctggcgc gcctcgacct
      601 cttcctggaa cagcgcgccg tcgatggcca cgtgggtgat gcgcggattg agcgcgctca
      661 tggtgttgag ggcctgcacc acgtcggggc agttctggca ggactgcgag aaataggtct
      721 cgaagtgata gtcgccgtcc agggcgcgga cccggtccag cgcctcggcg tcaaacttgg
      781 gcgggtggcc gcccacgtgc agcagggcca gcaccagcga ggtgaattcg tgacccagcg
      841 gcagaccggc aaagcgcaca tcggcgtcgc cggcggcgcg ggtgatggcg aaggacggct
      901 tgcgcgcgtc gccgccggtc aggctcaggc tgaccttgtc ggaggttccg gccacgtctt
      961 ccagcagggc gagcatgtcg cgcgagctgg cgccgtcgcc cagcgacgcg accagttcga
     1021 tcggctggcg caggttctgc agataggctt tcagctgggt cttcagacca tcgtccaaca
     1081 tggcgggctc cggggtggcg atgcgcgggg aggcgcggcg cgggatcttc aggaagaagg
     1141 gcgtcgccgc cagttccgtg ggctgacgga aaaggcggcg acgcggatca cgaccagagg
     1201 tcgcgaaggg gtcgtgtcgt tagatcttgc cgaccaggtc gagcgaaggc gcgagggtct
     1261 tttcgccttc ttcccacttg gcggggcaga cttcgcccgg gtgggcggcg acgtactggg
     1321 cggccttgat cttgcggatc agctcggcgg cgttgcggcc gatgccttcg gcggtgattt
     1381 ccatgaactg gatcacgcct tccggatcga tcaggaaggt gccgcggtcg gccaggccga
     1441 cgccttcgcg catgacgnnn nnnnnnntga cgtcgaagtt gttggtgatc aggcccgacg
     1501 ggtcgccgat catcgtgtac tggatcttgc cgatggcggg cgagctgtcg tgccaggcct
     1561 tgtgcgagaa gtgggtgtcg gtcgagaccg aatagacctc cacgcccatc ttctgcagtt
     1621 cgggatagtt gtcggccagg tcttcaagct cggtcgggca gacgaaggtg aagtcggcgg
     1681 gatagaagaa cacgacggac cacttgccct tcagatcggc gtcgctgacc tcgatgaact
     1741 tgccggcctt gtaggcctgg gcggtgaagg gtttgatggt ggtgttgatc aaggccatgg
     1801 agggctcccg cgaatggtgg ggccgatata ggggtcagcg aacgataggt ccaatcgatt
     1861 aaatcgttcc atgggatagg atcgatctat acgaagaggg ctttcgcggt taaggattgg
     1921 gtatgcttcc caccctgcgc cagctgcaat atctcaagct tctgtccgag cacggctcgt
     1981 tcagccgggc cgccgagagc gcccatgtca cccagcccac cctgtcggcg ggcatccagg
     2041 aactggagaa gatcctcggc gccgccgtgg tcgaccgcgc ccgctctggc gtcattctca
     2101 ccgccgccgg ccaggaagcc gtgcgaaggg cccaggatat cctgacccgc accgaggatc
     2161 tggtgcaggc ggcgcggggg gcgggcctgc cgctggccgg ccggttccgg ctgggcgtga
     2221 tcccgaccgt ggccccctat ctgttgccgc gcgccctgcc gctgctgcgc gaccgcttcc
     2281 ccaagctgcg attgttcctg cgcgaggacc tgacccagcg cctgatcgcg tccctgaagg
     2341 ccggcgccct ggacgccgcc ctgatcgccc tgccctacga catgtctggc ctggactggg
     2401 cccatgtcga ggatgacgaa ctgctggccg ccgcgcccgc caaccatccg atggccgcct
     2461 cggcgcgggt cgatcccgac agccttgtcg gtgaagacct gatcctgctg gaggacggcc
     2521 actgcctgcg cgaccacgcc ctggccgcct gcggactgga ggcgccctcc cgccacgcaa
     2581 atgggggctt tggcgatgag gagagcttcg ccgccacctc cctgcccacc ctggtgcaga
     2641 tgatcggctc gggcctgggc atctcgttcc tgccggccat ggcggtgcag gccggcctga
     2701 ccgaaaacgc cgccgtcacc gtgcggccgc tcgaccacgc ccattccagc cgcgagatcg
     2761 tggtggcctg gcgcgccggc tcgacccgcg cggccgaagg gcgtctgctg gccgaaaccc
     2821 tgcaggccgt ctgaccccgg cggcaagatc ggctgagccg tcgaacaaaa tattctcgat
     2881 atatctaaat aggctcttgc gcggccgcca atccgatata tctatcgttt cgatataacg
     2941 aaataggatc gaaacaacat gtttggacga caccaccatc accacggccg ccgcgatggc
     3001 ggtcattggg gcgctcacca cagccgccat cctttcggcg ttcacggcca cgaccatcac
     3061 ggacgcggtc cgggtcgggg cggcggccgc atgggccggt tcttcgacca cggcgacctg
     3121 cgcctggtgg ttctcaagct gatcgccgag cagcccagcc atggctatga gctgatcaag
     3181 gcggtcgaaa ccgcagcggg cggcgcctat acccccagcc cgggcgtgat ctatccaacc
     3241 ctgaccctgc tggaagagct gggctacatc accgtcatcg aggccggcgg cggcaagaag
     3301 ctgcacacca tcacccccga gggcgaagcc ttcctggaag ccaacgccca gccggtgaag
     3361 agcctgttcg agcgcatggc cgaggccgcc gctcgcagcg ccgccttctc gccgcagatc
     3421 ctgcgggccc gggaaaacct caagaccgcc ctgcggctga aactcaccgc cggccagctg
     3481 acggccgagc aggtcgacgc catcgccaag gcgctggacg acgcggccgg cgtcatcgaa
     3541 cgcatctgag gcgcgccgcg atgcagagcc atgcccgcct gaccaccgac aaggccgccc
     3601 gctacatgac ccagctggcc aagcactgga gccacaagtt cgcggtcacc ttcgatgaga
     3661 ccaccgccca cattccgctg ccgatcggcg aatgccgga
//
LOCUS       DAOMEO010000187         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2882_length_3692_cov_2.830080, whole genome shotgun sequence.
ACCESSION   DAOMEO010000187 DAOMEO010000000
VERSION     DAOMEO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_3692_cov_2.830080"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLH31_05545"
     CDS             complement(<1..633)
                     /locus_tag="PLH31_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88725.1"
                     /translation="MKPIVPRLLAAGLMAAPAGALAQSVEDLQIRAWRETYETGAWRP
                     EANSVLNANGNRYGVNLGCTAFSGKSVGIVIERSQKSRHPVSAYRGQAGPYCAPRGVG
                     LIEFADGSRWLGEVANRFNHWNLHSLPYPNGLGELRKADGSSQILRVKDKDKGPYGWD
                     VVQVVSASATTAGDPNRPVAWLTRPAPADWARLQPATGAGRVVLSCAIAAS"
     gene            complement(638..1447)
                     /locus_tag="PLH31_05550"
     CDS             complement(638..1447)
                     /locus_tag="PLH31_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88726.1"
                     /translation="MGAKRPATAVLLAAGLALPAPALAQSVEELQIRQWKETWEQAAW
                     TPRATMKVNNKGDRAQGIIFGCTLTADSTVALVIHRKAVSPHPVTAYRGATKGYCAPK
                     GVGIVDFADGGRWLGEVTNRYSKWKEHMLPYPDGLGELRKADGSTQILRVRSPEKGPY
                     GWDVVQVIASSTASAADATRPVAWLTRPAPPDWARLRPATGEGRVVLTCAIVRAGGID
                     CVTAESVSPELDAAALALSRLYKVDMAAEKDRVGYSFKLPFRFDPPTAPET"
     gene            complement(1437..>3052)
                     /locus_tag="PLH31_05555"
     CDS             complement(1437..>3052)
                     /locus_tag="PLH31_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88727.1"
                     /translation="RGDFKNKKPASGALMFASGAGVYGKFSSPTEAEAPTFSEGVSLM
                     LAKAEGAFPPGTYRAYAPRAVSDYRSLLNVNAHPERETLSRAPPRSLECPAPSLVPEG
                     WVVWSGGCRGGDKPRIRVYAPDARSLIEEQPGAEWPLTLASLRDGAVTRVVKAKALTA
                     DARPAPVGEAELVGERGRLIYRGGFLGQVPDGPGLCGTPAAEGGGEEPCFYAQGARTD
                     EIHLARREAAEAERQLRLAEAAARQQANARAEAQRRAQQAQAEANRRAYEAQQAERRR
                     MAQMEEEEEDDSSSGVNPIYQAMQELQGKYAAAAVDMQNQRAQTDAMQRNIEAQYRAR
                     QAEQARQQAQRNADVARQNAALNQQNADAARRAEIARQEAARVAQVRADSLTAARERQ
                     RAAMAQAQLQQRQAASAIGGGSASSALSGSSSAGAAARPGAAPSILTFTNNYGWKVGD
                     VSSTQNAQGVSISFTVASSTQAGKHYFRARICNTGSTPWSGGVRLTDSYPDRAHVTAQ
                     AQPGGCYALSEQFMASSKTIYVWVKNHGG"
     assembly_gap    3053..3062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3063..3584)
                     /locus_tag="PLH31_05560"
     CDS             complement(<3063..3584)
                     /locus_tag="PLH31_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88728.1"
                     /translation="MAQAVEQLRARFADPAWAPQKTMAHGAVVTLPPKVAKACGALHD
                     FRMTTSGRPHHIKEYRGAAKWCGFHPHNAPAKVAGLILYANGSYWLGEVSGTSTSPAM
                     PMPSGAGEAGYWSGMRILGVASPNIGSDKGRGVETFGFSTTERAIFANGAMRAVSLDA
                     SGSGKGEMRWPDGR"
BASE COUNT          469 a         1268 c         1332 g          613 t
ORIGIN      
        1 gctggcggcg atggcgcagc tcagcaccac ccggcccgcg ccggtcgccg gctgcaggcg
       61 ggcccagtcg gccggggcag gccgggtgag ccaggccacc ggtcggttcg ggtcgcccgc
      121 cgtcgtcgcg ctggccgaca ccacctgcac cacgtcccag ccataggggc ccttgtcctt
      181 gtccttgacc cgcaggatct ggctggaccc gtcggccttg cgtagttcgc ccagcccgtt
      241 gggataaggc agggaatgaa ggttccagtg gttgaaccgg ttagccacct cgcccagcca
      301 gcggctgccg tcggcgaact cgatcaggcc tacgccccgg ggtgcgcaat agggccccgc
      361 ctgcccgcga taggcgctga ccggatgtcg ggacttctgg cttcgctcga tgacgatgcc
      421 gaccgatttt cctgaaaagg cggtgcaacc caggttgacg ccgtagcgat tgccgttggc
      481 gttcagcacc gaattggctt ccggcctcca ggcgcctgtt tcgtatgttt cccgccacgc
      541 acggatctgc agatcctcga cgctctgggc cagggcgccg gccggcgccg ccatgaggcc
      601 ggccgcgaga aggcggggga caatcggctt catcggctca ggtctccggc gcggtcggcg
      661 ggtcgaaacg gaagggtagc ttgaagctat agccgacgcg atccttctcg gcggccatgt
      721 ccaccttgta gagccggctg agcgccaggg cggcggcgtc cagttcgggg gagacggact
      781 cggcggtgac gcagtcaatg ccgccggccc tgacgatggc gcaggtcagc accacgcggc
      841 cctctcccgt cgcgggtcgc aggcgcgccc agtcgggcgg ggcggggcgg gtgagccagg
      901 cgaccgggcg cgtggcgtcg gcggctgagg ccgtgctgga ggcgatgacc tgcacgacgt
      961 cccagccgta cggccccttt tcaggcgagc gcacacgcag gatctgggtg gagccgtcgg
     1021 ccttgcgcag ctctcccagg ccgtcggggt agggcagcat gtgttccttc cacttggaat
     1081 agcggttggt cacctccccc agccagcgac cgccatcggc gaagtcgaca atgcccacgc
     1141 ccttgggcgc gcaatagccc ttggtcgcgc cgcgataggc cgtcaccgga tggggcgaga
     1201 ccgccttgcg gtgaatgacc agggccacgg tgctgtcggc cgtcagggtg cagccgaaga
     1261 tgatgccctg ggcgcggtcg cccttgttgt tgaccttcat ggtggcgcgc ggcgtccagg
     1321 cggcctgttc ccaagtctcc ttccattgcc ggatctgcag ctcctcgacg ctctgggcca
     1381 gggcgggcgc cggcagggcc aggccggcgg cgagcaggac ggcggtcgca ggtcgcttag
     1441 cccccatggt ttttcaccca gacatagatg gttttggaac tggccatgaa ctgctctgac
     1501 agggcgtagc agccgcccgg ctgggcctgg gccgtgacat gggcgcggtc aggataggaa
     1561 tcggtcaggc gcaccccgcc gctccagggc gtcgacccgg tattgcagat gcgggcccgg
     1621 aaatagtgtt tgcccgcctg ggtgctggac gccacggtaa agctgatcga gacgccctgg
     1681 gcgttctggg tgctgctgac gtcgccgacc ttccagccgt aattgttggt gaaggtcagg
     1741 atcgaggggg ctgcgccggg ccgggccgcg gcgccggctg atgacgatcc gctgagcgcc
     1801 gaggacgccg atccgccgcc gatcgcgctg gccgcctggc gctgttggag ctgggcctgg
     1861 gccatggcgg cgcgctggcg ctcgcgggcc gccgtcaggc tgtcggcgcg gacctgggcg
     1921 acgcgggccg cctcttgccg ggcgatctcg gcgcggcggg cggcgtcggc gttctgctgg
     1981 ttgagggcgg cgttctggcg agccacatcg gcgttgcgct gggcctgctg gcgcgcctgc
     2041 tcggcctgcc gggcccggta ctgcgcctcg atgttgcgct gcatggcgtc ggtctgggcg
     2101 cgctggttct gcatatcgac ggcggcggcc gcgtacttgc cctgcaactc ctgcatggcc
     2161 tgatagatcg ggttcacgcc ggaggaggag tcgtcttcct cctcttcctc catctgggcc
     2221 atgcgccgcc gttcggcctg ctgggcctca taggcccgcc ggttggcctc ggcctgggcc
     2281 tgctgggcgc gtctctgggc ctcggcgcgg gcattggcct gctgccgggc ggccgcttcg
     2341 gccagcctca gctgtcgctc ggcttcggcg gcttcgcgcc gcgccaggtg gatctcgtcg
     2401 gtgcgcgcgc cctgggcgta gaagcacggc tcttcgccgc cgccctcggc cgccggggtg
     2461 ccgcacaggc ccggaccatc cgggacctga cccaggaacc cgccgcgata gatgagcctg
     2521 ccacgctcgc ccaccagctc ggcctctccc acgggagcgg ggcgggcgtc ggcggtgagg
     2581 gccttggcct tgaccacccg ggtcacggcg ccgtcgcgca ggctggccag ggtcaatggc
     2641 cactctgcgc ccggctgctc ctcgatcagg ctgcgagcgt cgggtgcgta gaccctgatg
     2701 cgcggcttgt cgccgccgcg gcagccaccg ctccagacca cccagccttc cggcacgagg
     2761 ctgggcgcgg ggcattccag cgaccttggc ggcgcgcggc tcaaggtctc ccgctcggga
     2821 tgggcgttga cgttcagcag gctgcgatag tcgctgaccg cgcgcggcgc ataggcccgg
     2881 taggttccgg gcggaaaggc gccctcggcc ttggccagca tcagggaaac gccttcgctg
     2941 aaggtgggag cctccgcctc ggtcgggctt gagaacttgc catacacgcc ggccccggag
     3001 gcgaacatga gggctcctga cgcgggcttc ttgttcttga aatctccccg gannnnnnnn
     3061 nngcggccgt cgggccagcg catctcgccc ttgcctgaac cgctggcgtc cagcgagacc
     3121 gcgcgcatcg ccccgttggc aaagatcgct cgctcagtcg tcgaaaagcc gaaggtctcg
     3181 acgccgcgcc ccttgtccga gccgatgttg ggcgaggcga cgcccaggat gcgcattcca
     3241 ctccaatagc ccgcctcacc ggccccgctg ggcatgggca tggcggggct ggtcgatgtg
     3301 ccgctgacct cacccaacca gtagctgcca ttggcgtaga ggatcaggcc ggccaccttg
     3361 gccggggcgt tgtgcggatg gaaaccgcac cacttggctg ccccgcgata ttccttgatg
     3421 tggtgcggcc gcccgctggt ggtcatgcgg aagtcgtgca gcgcgccgca ggccttcgcc
     3481 accttgggcg gcagggtgac cacggcgccg tgagccatgg tcttctgcgg cgcccaggcg
     3541 ggatcggcga accgggcgcg caactgctcc accgcctggg ccatcaccgc gcccctttcc
     3601 gtctggggcg cctcggccgg ccagggcgtc tggaaattgg ataaggacgc ggcgggcgcg
     3661 gcgctggctg cgctctgctc ggcctccatg cc
//
LOCUS       DAOMEO010000188         3676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2895_length_3676_cov_2.865783, whole genome shotgun sequence.
ACCESSION   DAOMEO010000188 DAOMEO010000000
VERSION     DAOMEO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_3676_cov_2.865783"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="prs"
                     /locus_tag="PLH31_05565"
     CDS             complement(<1..425)
                     /gene="prs"
                     /locus_tag="PLH31_05565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HQR88729.1"
                     /translation="MKLLSGNSNRPLSQAIAEYLDMPLTRAQVRRFADLEVFVTIDEN
                     VRGEDVFVLQSTSYPANDNLMELLICIDALKRASGKRITAVLPYFGYARQDRKTGGRT
                     PISAKLVANLITRSGADRVLTMDLHAGQIQGFFDIPTDNL"
     gene            complement(544..1329)
                     /gene="pgeF"
                     /locus_tag="PLH31_05570"
     CDS             complement(544..1329)
                     /gene="pgeF"
                     /locus_tag="PLH31_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQR88730.1"
                     /translation="MPHSPLPTIQSPLLAAVPGIRHAFFTRQGGVSTGIYDSLNVGRG
                     SKDDPADVVENRARAARWFGVAPEDLNTCYQIHSTLAIKADGSWGDVRPEGDAVVTKT
                     PGVVCGALAADCAPVLIVDPEANIVAAAHAGWRGALDGVVQAAVDAMVELGGDPERMV
                     AAVGPCIGPDSYEVGLEFLHRFEADCPGSGQFFRPGATDDKRMFDLPAFVLDRLRTAG
                     IEQREWVGRDTRVEEVLFFSNRRAFLNGEGDYGRLLSAIAIEA"
     gene            complement(1331..2416)
                     /locus_tag="PLH31_05575"
     CDS             complement(1331..2416)
                     /locus_tag="PLH31_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR88731.1"
                     /translation="MSLLDRLKAQIAQDGPIGVAEYFTRCLHDPQDGYYATRPALGGA
                     GGDFITAPLVSQMFGELIGLWAVETWTRMGRPSPFRLVEMGPGDGTLMSDLLRAARLA
                     PDFMAAVDLWLVEVSPPLRALQAQRLAGAAPQWADRLETVPGGAPMILVANEVLDCLP
                     ARQFVRTETGWAERVIGLDDAGGLAFGLRGLSAPRRGEPSPPEAGDTPVGAILEASPA
                     QAALGAEIGHRLAADGGAALLIDYGRDAPGPGDTLQALQGHAKVDPLQTPGQADLTVW
                     ADFPAVLAAGVEAGAKAGPILEQAAFLAMLGIEARAKALAAARPDRADQIARQLDRLT
                     GQAQMGALFKAVCLHAPGFTPPAFEVA"
     gene            complement(2403..3299)
                     /gene="lgt"
                     /locus_tag="PLH31_05580"
     CDS             complement(2403..3299)
                     /gene="lgt"
                     /locus_tag="PLH31_05580"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQR88732.1"
                     /translation="MIFPDIDPVVHIGPLALQWGPLAIRWYALAYVAGILLGWRYAVR
                     MVKTPSLWGGRTPTATPIQIDDLVLWLTLGIILGGRLGYILFYMLMNEGQRGGLAANP
                     LAAFKIWEGGMSFHGGFLGVCVAILLFARQQKIDVLKLGDLIAPVAPIGIFFGRVANY
                     INGELWGRVTDHPFGVIFCNQTIQAANGGRCPAGPDPRHPSQLYEAALEGLLLFAILW
                     VATHRLKWLQRRGALIGTFLVGYGLFRALLENVRNPDEGMPMFPFGLTMGMILSIPML
                     LVGAWLLWKALREPADPAHEPA"
     gene            3413..>3676
                     /locus_tag="PLH31_05585"
     CDS             3413..>3676
                     /locus_tag="PLH31_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQR88733.1"
                     /translation="MHSQNPFLDEFAKLTQAAMGIAQTAGEEAKTAMRAQADRLAAEF
                     DLIRRDDFEALKAEVAALREEVATLKAKKPAAKAVGKTVKVAAT"
BASE COUNT          623 a         1246 c         1282 g          525 t
ORIGIN      
        1 aggttgtcgg tggggatgtc gaagaagccc tggatctggc cggcgtgcag atccatcgtc
       61 agcacccggt cggcgcccga gcgggtgatc aggttggcca ccagcttggc cgagatcggc
      121 gtacgaccgc cggtcttccg gtcctggcgg gcgtaaccga aataggggag gacggcggtg
      181 atccgcttgc ccgacgcgcg cttcagggcg tcgatgcaga tcagcagctc catcaggttg
      241 tcgttggccg gatagctggt cgactgcagg acgaagacat cctcgccgcg cacgttctcg
      301 tcgatggtga cgaacacctc caggtcagcg aaccggcgca cctgggcgcg ggtcagcggc
      361 atgtcgagat actcggcgat ggcctgggaa agcgggcggt tcgagttgcc ggacagcagc
      421 ttcatggggt cgctcggagc agggggtgcg cgttgccggg ccttctagca gtgatcggcc
      481 ccggagcaag accttaacgg ccggcgtgac ggttcggtga cgttttgtgg ctttcgggtt
      541 ggcctacgcc tcgatcgcga tcgccgacag caggcggccg tagtcgcctt cgccgttcag
      601 gaaggcgcgg cggttggaga agaacaggac ttcctcgacg cgggtgtcgc ggcccaccca
      661 ttcgcgctgc tcgatccccg ccgtgcgcag gcggtccagc acgaaggcgg gcaggtcgaa
      721 catccgcttg tcgtcggtcg cgcccggccg gaagaactgg cccgagcccg ggcagtctgc
      781 ttcgaagcgg tgcaggaact ccagccccac ctcgtaggag tccgggccga tgcagggacc
      841 gacagcggcg accatgcgtt cgggatcgcc gcccagctcg accatggcgt cgacggcggc
      901 ctgcacgacc ccgtccaggg ctccgcgcca gccggcatgg gcggcggcga cgatattcgc
      961 ctcgggatcg acgatcagca caggcgcgca gtcggccgcc agggcgccgc agaccacgcc
     1021 aggggtcttg gtgaccaccg cgtcgccctc gggtcggacg tcgccccacg agccatcggc
     1081 cttgatcgcg agggtcgagt ggatctggta gcacgtgttg aggtcttcgg gcgcgacccc
     1141 gaaccagcgc gcggcgcggg cccggttctc gaccacgtcg gcgggatcgt ccttgctgcc
     1201 gcgcccaacg ttcaggctgt catagatccc ggtcgagacc ccgccctgac gcgtgaagaa
     1261 ggcgtggcga atgcccggta cggcggccag cagcggcgac tggatcgtag gcagtgggct
     1321 gtgcggcatg tcaggcaacc tcgaaggcag gcggcgtgaa accgggtgcg tgcaggcaca
     1381 cggccttgaa caacgcgccc atctgcgcct gaccggtcag gcgatcaagc tgtcttgcga
     1441 tctggtcggc gcgatcgggt cgggcggcgg ccagagcctt agcccgcgct tcgatgccca
     1501 gcatcgccag gaaggcggcc tgctcaagga tcggcccggc tttcgcgccg gcttccacgc
     1561 ccgccgccag cacggcgggg aagtcggccc agacggtcag gtcggcctgg ccgggggtct
     1621 gcagcgggtc gaccttggca tggccctgga gggcctggag tgtgtcgccc ggtcccggcg
     1681 cgtcgcggcc atagtcgatc agcagcgccg cgccgccgtc ggcagcgagg cgatggccga
     1741 tctcggcgcc gagggctgcc tgggcggggg aggcttcgag gatggcgccg accggagtat
     1801 cgccggcctc cggcggcgat ggctcgccgc gcctgggcgc tgacaggccg cgaagaccaa
     1861 acgccagccc gccagcgtca tccagcccga ttacccgctc ggcccagccc gtctctgtgc
     1921 ggacaaactg ccgggcgggc aggcagtcca gcacctcatt ggcgaccagg atcatcggcg
     1981 cgccgccggg aaccgtctcc agccgatcgg cccactgcgg cgcagcgccc gccaggcgct
     2041 gcgcctgcaa cgcgcgtaaa ggcggcgaga cctcaaccag ccagaggtca acggccgcca
     2101 tgaaatccgg cgccagcctc gcagccctca gaaggtcgct catgagcgtc ccgtcgccag
     2161 gccccatctc gaccagccgg aagggcgagg gccggcccat gcgggtccag gtttccaccg
     2221 cccacaggcc gatcagctcg ccaaacatct ggctgaccag gggcgcggtg atgaagtcgc
     2281 cgccggcgcc gccaagcgcg ggccgggtgg cgtagtagcc gtcctgcgga tcgtgcagac
     2341 agcgggtgaa atactcggcg acgccgatcg gcccgtcctg ggcgatctgc gccttcagac
     2401 gatcaagcag gctcatgagc cgggtcggcg ggttcgcgca gggccttcca cagcagccag
     2461 gcgccgacca gcagcatcgg gatcgacagg atcatcccca tggtcaggcc gaaggggaac
     2521 atcggcatgc cctcatcggg attgcgtacg ttctccagca gggcgcggaa caggccgtag
     2581 ccgaccagga aggttccgat cagcgcgccg cgacgttgta gccatttcag tcggtgggtc
     2641 gccacccaca ggatcgcaaa cagcagcagc ccttccagcg ccgcctcata gagctggctg
     2701 ggatggcgcg gatcggggcc ggcggggcag cgaccgccat tggcggcctg gatagtctgg
     2761 ttgcagaaga tcaccccgaa cgggtggtcg gtgacccggc cccacagctc gccattgata
     2821 tagttggcca cccgcccgaa gaagatgccg atcggcgcga ccggcgcgat caggtcgccc
     2881 agcttcagca catcgatctt ctgctgccgg gcgaacagca ggatggcgac gcagactccc
     2941 aggaagccgc catggaagga catgccgccc tcccagatct tgaacgccgc cagcgggttg
     3001 gcggccagac cgccgcgctg gccttcgttc atcagcatgt agaacaggat atagcccagc
     3061 cggccgccca ggatgatgcc cagggtgagc cacagcacca ggtcgtcgat ctgaatcggc
     3121 gtcgcggtcg gcgtgcggcc gccccacagc gacggcgtct tcaccattcg tacggcgtag
     3181 cgccagccca gaaggatgcc ggccacatag gccagggcgt accagcggat cgccagcggc
     3241 ccccattgca gggccagcgg gccgatgtgc acgaccgggt cgatatcggg aaagatcacg
     3301 cggcaggctc cgttccggac agatcgtcgc agccatagcc ggttcggcct tcgctttcac
     3361 cccctgcatc ccatataagg agggcggaca cgacgcccgc cttggaacct cgatgcacag
     3421 ccagaacccc tttctcgacg aattcgccaa gctgacccag gccgccatgg gcatcgccca
     3481 gacggctggc gaggaagcca agaccgcgat gcgggcccag gccgatcgcc tggcggctga
     3541 attcgacctg atccgccgcg acgatttcga ggcgctgaag gccgaggtgg cggcgctgcg
     3601 cgaggaagtc gcgacgctga aggccaagaa gcccgccgcc aaagccgtcg gcaagaccgt
     3661 caaagtcgcc gccacc
//
LOCUS       DAOMEO010000189         3661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2904_length_3661_cov_2.316972, whole genome shotgun sequence.
ACCESSION   DAOMEO010000189 DAOMEO010000000
VERSION     DAOMEO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2904_length_3661_cov_2.316972"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLH31_05590"
     CDS             <1..630
                     /locus_tag="PLH31_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR88734.1"
                     /translation="IVGAQVALGTGLALANHYKGNGNVSYAYFGDGAANQGQVYESFN
                     MAQLWKLPVVYVIENNQYAMGTSVERSASETAFHKRGVSFRIPGEEVDGMDIAAVREA
                     GARATEHARSGLGPYILEMKTYRYRGHSMSDPAKYRTKEEVDEVKQTRDPIDHIKERL
                     AKAGVTEDDLKGIDAEVKRIVAEAAEFARTSPEPDPSELYTDVYLEAAQ"
     gene            627..>991
                     /locus_tag="PLH31_05595"
     CDS             627..>991
                     /locus_tag="PLH31_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR88735.1"
                     /translation="MSVDILMPALSPTMEEGTLAKWLVKEGDTVKAGDVIAEIETDKA
                     TMEVEAVDEGVVEAILVPGGTENVKVNTLIARLKGEGEAASAAPVPAAQAAPVAATSV
                     AAAAPAVSAGASTFADPEIP"
     assembly_gap    992..1001
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1002..1921
                     /locus_tag="PLH31_05600"
     CDS             <1002..1921
                     /locus_tag="PLH31_05600"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HQR88736.1"
                     /translation="ERVFLMGEEVAQYQGAYKVSRDLLQEFGAKRVIDTPITEHGFAG
                     MGVGAAMAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGQIKSSIVFRGPNGAAS
                     RVGAQHSQDYAAWYGNVPGLKVIAPYDAADAKGLLKAAIRDPNPIVFLEHEMMYGLEF
                     DIPDVEDHVVPIGKAKVRREGTDVTLVAYSRMVGFCLKAADELAKEGISAEVVDLRTI
                     RPMDHDTILQSVKKTNRLVTAEEGWGPMGVGAEIVARITEHGFDYLDAPPLRVHQEDV
                     PLPYAANLEALSLPSVDKIVSAAKKVCYR"
     gene            1921..2070
                     /locus_tag="PLH31_05605"
     CDS             1921..2070
                     /locus_tag="PLH31_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88737.1"
                     /translation="MKMRLTSGGAAAIVAYLSGVSRFDFGQEQHLAPARDRADDGPAD
                     YVPNA"
     gene            2083..3357
                     /locus_tag="PLH31_05610"
     CDS             2083..3357
                     /locus_tag="PLH31_05610"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HQR88738.1"
                     /translation="MSIDILMPALSPTMEEGTLAKWHVKVGDTVKAGDVIAEIETDKA
                     TMEVEAVDEGVVEAILVDAGTENVKVNALIAKLVGEGESPAPALKAAPAAEEPKAAPP
                     APAAAPQAAAALVAQIGDRVLASPLARRLAQAAGLDLKTVKGTGPHGRVVKADVEAAG
                     KGGAAPAKAATSTSAPAAAAAPRQALSLEQMGIPAGSYDLVPLDGMRKTIARRLTDSF
                     RDVPHFPLTIDLEIDGLLAARAKINSLLADQGVKVSVNDIIIKAVAVALKRVPEANAS
                     YTPEGIAMHKHADIAVAVAIDGGLITPIVRAAETKGLAQISAEVKDLAARAKAKKLKP
                     EEFQGGTFSVSNLGMFGIKAFASIINEPQGAIMSVGAGEQRPVVKNGQLAVATVMTVT
                     LTCDHRVVDGAIGAKFLAAFKPLIEEPLTLIV"
     gene            3383..>3661
                     /locus_tag="PLH31_05615"
     CDS             3383..>3661
                     /locus_tag="PLH31_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQR88739.1"
                     /translation="MSVYDYSAKTLDGHDVSLADYRGQVLLIVNTASKCGFTPQYEGL
                     EALYRQYKDRGLSILGFPCNQFGAQEPGNAEEIASFCSLTYDVTFPMLA"
BASE COUNT          690 a         1213 c         1173 g          575 t
ORIGIN      
        1 atcgtcggcg cgcaggtcgc tctgggcacg ggccttgcct tggccaacca ctacaagggc
       61 aatggcaacg tctcgtacgc ctatttcggt gacggcgcgg ccaaccaggg ccaggtctat
      121 gagagcttta acatggccca gctgtggaag ttgccggtcg tgtatgtgat cgagaacaac
      181 caatacgcca tgggcaccag cgttgagcgt tcggcgtcgg aaaccgcctt ccacaagcgt
      241 ggcgtatcgt tccggattcc gggcgaggaa gtcgacggaa tggatatcgc cgccgttcgc
      301 gaagccggcg cccgcgcgac cgagcacgct cgcagcggcc tgggtcccta catccttgag
      361 atgaagacct atcgctatcg tggtcactcg atgtcggatc cggccaagta ccgtaccaag
      421 gaagaggtcg atgaggtcaa gcagacccgc gatccgatcg atcacatcaa ggaacgcctg
      481 gccaaggctg gcgtcaccga ggacgacctg aagggcatcg acgccgaggt gaagcgcatc
      541 gtcgccgaag ccgccgaatt cgcgcgaacc agccctgaac ccgatccgtc cgaactgtac
      601 acagacgtct acttggaggc cgcccagtga gcgtggacat cctgatgccc gccctctcgc
      661 ccacgatgga agagggcacg ctggccaagt ggctggtcaa ggaaggcgac accgtcaagg
      721 ccggcgacgt gatcgccgag atcgaaaccg acaaggcgac gatggaagtc gaagccgtcg
      781 atgaaggcgt cgtcgaggcc atcctggtcc ccggcggcac cgagaacgtc aaggtcaaca
      841 cgctgatcgc gcgtctgaag ggcgagggcg aggcggcttc ggccgctccg gtgcccgcag
      901 cccaggctgc tcccgtggct gccacgtcgg tcgctgcggc cgctccggct gtcagcgccg
      961 gcgcctcgac cttcgctgat ccagaaattc cnnnnnnnnn nacgaacgcg tgttcctgat
     1021 gggcgaggaa gtcgcgcagt accagggcgc ctacaaggtt agccgcgacc tgctccagga
     1081 attcggcgcc aagcgcgtca tcgacacccc gatcaccgag cacggctttg ccggcatggg
     1141 cgtcggcgcg gcgatggcgg gtctgaagcc gatcgtcgag ttcatgacct ggaacttcgc
     1201 catgcaggcg atcgaccaca tcatcaactc ggccgccaag acgctctata tgtcgggcgg
     1261 tcagatcaag tcgtcgatcg tgttccgcgg ccccaacggc gccgcgtccc gcgtgggcgc
     1321 ccagcacagc caagactatg ctgcctggta tggcaacgtt ccgggtctga aggtcatcgc
     1381 gccctatgac gcggctgacg ccaagggcct gctgaaggcc gccatccgcg acccgaaccc
     1441 gatcgtcttc ctcgaacacg agatgatgta cggcctcgaa ttcgacatcc ccgatgtcga
     1501 ggaccacgtc gtgccgatcg gcaaggccaa ggtccgtcga gagggcacag acgtcaccct
     1561 ggtggcctac agccgcatgg tcggcttctg cctgaaggcc gctgacgaac tggccaagga
     1621 aggtatctcg gccgaggtcg tcgatctgcg gaccatccgt ccgatggatc acgacaccat
     1681 cctgcagagc gtcaagaaga ccaaccgtct ggtgactgcc gaggaaggct ggggcccgat
     1741 gggcgtcggc gccgagatcg tcgcgcgcat caccgagcac ggcttcgact acctggacgc
     1801 cccgccgctg cgggtccatc aggaagacgt tccgctgccc tatgcggcca atctggaagc
     1861 cctctcgctg ccgtcggtcg acaagatcgt ctcggccgcc aagaaggtct gctaccgctg
     1921 atgaagatgc ggctcacgtc tggcggtgcg gcggcgatcg ttgcatacct gtcgggcgtg
     1981 agccgtttcg atttcggcca agaacaacac ctcgccccgg cgcgagatcg cgcggacgac
     2041 ggcccggccg actacgtccc gaacgcttga ggaagaagct caatgtcgat cgacatcctg
     2101 atgcccgccc tgtcgcccac catggaagag ggcaccctcg ccaagtggca cgtgaaggtg
     2161 ggcgacaccg tcaaggccgg cgacgtgatc gccgagatcg agaccgacaa ggccacgatg
     2221 gaagtcgagg ccgtcgacga gggcgtggtc gaggccatcc tggtcgacgc cgggaccgag
     2281 aacgtcaagg tcaacgccct gatcgccaag ctggtcgggg agggcgaaag cccggctccc
     2341 gcgctgaagg ctgcccccgc cgctgaagag ccgaaggctg caccgcccgc accggccgct
     2401 gcgcctcagg ccgccgccgc tctagtcgct caaatcggcg atcgtgtgtt agcgtcaccg
     2461 ctggcccgcc gtctggctca ggccgccggc cttgacctga agactgtcaa gggcaccggt
     2521 ccgcatggcc gggtggtcaa ggccgatgtc gaggccgcag gcaaaggcgg cgctgcgcct
     2581 gccaaggccg cgacttcaac ctcggctccg gccgctgccg ccgcgccgcg tcaggcgctg
     2641 tcgctggagc agatgggcat ccccgccggc tcctacgacc tggttccgct ggacggcatg
     2701 cgcaagacca tcgcgcgccg cctgaccgat agcttccgtg acgtgccgca cttcccgctg
     2761 accatcgatc tcgagatcga cggcctgctg gcggcccgcg ccaagatcaa tagcctgctg
     2821 gcggatcagg gcgtgaaggt gtcggtcaat gacatcatca tcaaggccgt tgccgtggcc
     2881 ctcaagcgcg tgccggaggc caacgcctcc tacacgccgg aaggcatcgc catgcataag
     2941 cacgccgaca tcgcggtggc cgtagccatc gacggcggcc tgatcacgcc gatcgtccgc
     3001 gccgccgaga ccaagggcct ggcccagatc tcggccgagg tgaaggacct ggcggcgcgc
     3061 gccaaggcca agaagctgaa gcccgaggaa ttccagggcg gcaccttctc ggtctccaac
     3121 ctgggcatgt tcggcatcaa ggccttcgcc tcgatcatca acgagccgca gggcgccatc
     3181 atgagcgtcg gggccggcga gcagcgcccg gtggtcaaga acggccagct ggccgtggcg
     3241 accgtaatga ccgtcacctt gacctgcgat caccgcgtgg tcgatggcgc tattggcgcc
     3301 aagttcctgg cggccttcaa gccgctgatc gaagagccgc tgaccctgat cgtctgatca
     3361 ctggaagggg caggagagcg ccatgtcggt ctatgactac tcggccaaga cgttggacgg
     3421 ccacgacgta agcctggccg actatcgtgg tcaggtgctg ctgatcgtca acacggccag
     3481 caagtgcggt ttcaccccac agtacgaagg ccttgaggcg ctctatcgcc aatacaagga
     3541 tcgcggcctg agcatcctgg gcttcccgtg caaccagttc ggagcccagg agcccgggaa
     3601 cgccgaagag atcgccagct tctgctcgct gacctatgac gtgaccttcc cgatgctggc
     3661 c
//
LOCUS       DAOMEO010000190         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2909_length_3652_cov_3.359744, whole genome shotgun sequence.
ACCESSION   DAOMEO010000190 DAOMEO010000000
VERSION     DAOMEO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2909_length_3652_cov_3.359744"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..1446
                     /gene="leuC"
                     /locus_tag="PLH31_05620"
     CDS             10..1446
                     /gene="leuC"
                     /locus_tag="PLH31_05620"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQR88740.1"
                     /translation="MSGKTLYDKIWDAHVVSETDGEAILYIDLHLIHEVTTPQAFAGL
                     RAAGRKVRRPDRTLAVADHNIPTEGQALGVDAVADEEARLQLQTLARNVKDNGIPFFP
                     MGDIRNGIVHVVGPEQGRTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
                     LRQKKSKNMLVRVDGQLGPGVTGKDIALAVIGEIGTAGGTGYVIEFAGEAISALSMEG
                     RMTLCNLTIEGGAKAGLIAPDDKTYAYIQGKPAAPKGAAWDMAKSHWKSFFTDPDAVF
                     DRIVTIDASSLVPMVTWGTSPEDVIPVTGNVPDPESFPTPDKRAAAHRALDYMGLTAG
                     QPISEARIDRVFIGSCTNSRIEDMRAAAAVVQEAFLHGRLVADHVTAMVVPGSGLVKE
                     QAEEEGLDAIFKAAGFDWREPGCSMCLAMNPDKLQPGERCASTSNRNFEGRQGRAGRT
                     HLVSPAMAAAAAIAGHLVDVRTFLTETV"
     gene            1446..2054
                     /gene="leuD"
                     /locus_tag="PLH31_05625"
     CDS             1446..2054
                     /gene="leuD"
                     /locus_tag="PLH31_05625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQR88741.1"
                     /translation="MQAFTRLDGRAVPLSLANIDTDQIIPKQFLKTVEREGLAKGLFY
                     DFRFDGEGNEISDFVLNRPEYKGASVLIAGDNFGCGSSREHAPWALMDFGIMCVISTS
                     FADIFNNNCFNNGLLPVVLAPDEIAVLMDEALGGNHMVSIDLEAQTVISPSGKSFSFQ
                     IDPVRKDKMLRGLDAIGETMMHGGDIDLFESKRAISEPWLEV"
     gene            2193..2495
                     /locus_tag="PLH31_05630"
     CDS             2193..2495
                     /locus_tag="PLH31_05630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HQR88742.1"
                     /translation="MTIILAGTVRIAPERLADLRPHLRAQVEGSRAEPGCLDYALTED
                     PLDPGLIRVYEHFVDEDALAFHRASPHMAAWRACWPELGLGERRMSSFDVSDYREI"
     gene            2507..3559
                     /gene="leuB"
                     /locus_tag="PLH31_05635"
     CDS             2507..3559
                     /gene="leuB"
                     /locus_tag="PLH31_05635"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQR88743.1"
                     /translation="MSTLLLLPGDGIGPEVCAQVRRVAAALTPDLVVEEAIYGGASYD
                     AHGTPLTDEVRERALASGAVLMGAVGGPKWKDAPRHLRPEAGLLNLRKAMDVYANLRP
                     AYCFEALADASSLKRELVSGLDIMFVRELVGGVYFGQPRGIEDLPDGQKKGFDTAVYT
                     TSEIERVGRVAFELARGRTNRVHSAEKSNVMESGLLWKQVITDLHAREYPDVQLEHIL
                     ADNCAMQLVRAPKQFDVIVTDNLFGDILSDAAAMLTGSLGMLPSAALGAAGKPGLYEP
                     IHGSAPDIAGQGVANPLAAILSFEMALRWSLGRAEAADVLLAAVKAALDGGARTRDLG
                     GSLSTVEMGDAVLARL"
BASE COUNT          611 a         1294 c         1187 g          560 t
ORIGIN      
        1 atccccgcca tgtccggcaa aaccctttat gacaagatct gggacgccca cgtcgtcagc
       61 gaaaccgacg gcgaggccat cctctatatc gacctgcacc tgatccacga ggtcacgact
      121 ccgcaggcct tcgcgggcct tcgcgcggcc ggtcgcaagg tgcgccgtcc cgaccgcacc
      181 ctggcggtgg ccgaccacaa tatcccgacc gaaggccagg ccctgggcgt tgacgccgtg
      241 gccgacgaag aggcgcggct gcagctgcag accctggccc gcaacgtcaa ggacaacggc
      301 atcccgttct tcccgatggg cgatatccgc aacggcatcg tccacgtggt gggtcccgag
      361 cagggccgca cacagccggg catgaccatc gtctgcggcg acagccacac ctcgacccac
      421 ggcgccttcg gggccctggc ccacggcatc ggcacgtccg aggtcgagca cgtcctggcc
      481 acccagaccc tgcgccagaa gaagtcgaag aacatgctgg tccgcgtcga cggccagctg
      541 ggcccaggcg tcaccggcaa ggacatcgcc ctggccgtga tcggcgagat cggcacggcc
      601 ggcggcaccg gctatgttat cgagttcgcc ggcgaggcca tctcggccct gtcgatggaa
      661 ggccgcatga ccctgtgcaa cctcaccatc gagggcggcg ccaaggccgg cctgatcgcg
      721 ccggatgaca agacctacgc ctatatccag ggcaagcccg ccgcccccaa gggcgcggcc
      781 tgggacatgg ccaaaagcca ctggaagagc ttcttcaccg atccggacgc cgtctttgac
      841 cgcatcgtga cgatcgacgc ctcgtccctg gtcccgatgg tcacctgggg caccagcccc
      901 gaggacgtga tcccggtcac cggcaacgtc cccgacccgg aaagctttcc cacccccgac
      961 aagcgcgccg ccgcccaccg ggcgctggac tatatggggc tcactgccgg ccagccgatc
     1021 tcggaggccc gcatcgaccg cgtcttcatc ggctcgtgca ccaacagccg gatcgaggac
     1081 atgcgcgccg ccgccgccgt ggtgcaggaa gccttcctgc acggccgcct ggtggccgac
     1141 cacgtcaccg ccatggtcgt gccgggctcg ggcctggtca aggaacaggc cgaagaggaa
     1201 ggactggacg ccatcttcaa ggccgccggt ttcgactggc gcgaaccggg ctgctcgatg
     1261 tgcctggcca tgaaccccga caagctgcag ccaggcgagc gctgcgcctc gaccagcaac
     1321 cgcaatttcg aaggccgcca gggccgggcc ggtcgcaccc acctggtctc gcccgccatg
     1381 gcggcggcgg cggcgatcgc cggccacctt gtcgacgtgc gcaccttcct gacggagacc
     1441 gtctgatgca ggccttcacc cgcctcgacg gccgcgccgt cccgctgtcg ctggccaata
     1501 tcgacaccga ccagatcatc cccaagcagt tcctcaaaac cgtggagcgc gagggcctgg
     1561 ccaagggcct gttctatgac ttccgcttcg acggcgaggg gaacgagatc agcgacttcg
     1621 ttctgaaccg gccggagtac aagggcgcca gcgtactgat cgccggcgac aatttcgggt
     1681 gcggctcctc gcgcgagcac gccccctggg ccctgatgga cttcgggatc atgtgcgtga
     1741 tctcgaccag cttcgccgac atcttcaaca acaactgctt caacaacggc ctgctgccgg
     1801 tggtgctggc gcccgacgag atcgccgtcc tgatggacga ggcgctaggc ggcaaccaca
     1861 tggtcagcat cgacctggaa gcccagacgg tgatctcgcc ttcgggcaag agcttcagct
     1921 tccagatcga cccggtacgc aaggacaaga tgctgcgcgg cctggacgcc atcggcgaga
     1981 cgatgatgca cggcggcgac atcgacctgt tcgaaagcaa gcgggcgatc agcgagccgt
     2041 ggctggaagt ttagacgtaa gggctgtcat cccggacgag cgcgtcgcgc gaagatccgg
     2101 gaccgacgcc tgagcgccgc gttccagggc ggtcccggat cggcggccct gcgggccttg
     2161 tccgggatga caaccttttt tgagatgaac ccatgaccat catcctcgcc ggcaccgtcc
     2221 gcattgcgcc cgaacgtctg gccgacctgc ggccgcatct gcgcgcccag gtcgagggca
     2281 gccgggccga gccgggctgc ctggactacg ccctgaccga ggatccgctc gatccgggcc
     2341 tgatccgggt ctatgagcat ttcgtcgacg aggacgccct ggcctttcac cgcgccagcc
     2401 cgcacatggc cgcctggcgc gcctgctggc cggagctggg tctgggcgag cgccgaatgt
     2461 cctcgttcga cgtttcagac taccgcgaaa tctaggaagc cttccgatgt ccacgcttct
     2521 cctgctgccc ggcgacggga tcggtcccga ggtttgcgcg caggtgcgcc gcgtggcggc
     2581 cgcgctgacg cccgacctgg tggtcgaaga ggccatctat ggcggcgcca gctatgacgc
     2641 ccacggcacg cccctgaccg acgaggtccg tgaacgcgcc ctggccagcg gcgccgttct
     2701 gatgggcgcc gtcggcggtc ccaagtggaa ggacgccccg cgccacctgc gcccggaagc
     2761 cggccttctg aacctgcgca aggccatgga cgtctatgcc aacctgcgcc cggcctattg
     2821 ttttgaggcc ctggcggatg cgtcgagcct gaagcgcgag ctggtttcgg gcctggacat
     2881 catgttcgtg cgcgaactgg tcggcggcgt ctatttcggc cagccgcgcg gcatcgagga
     2941 cctgccggac ggccagaaaa agggctttga cacggccgtc tatacgacca gcgagatcga
     3001 gcgcgtcgga cgcgtggcct tcgagctggc ccggggccgc accaaccggg ttcactcggc
     3061 cgagaagtcc aacgtcatgg aatcgggcct gctgtggaag caggtcatca ccgacctgca
     3121 cgcccgcgaa tatccggacg tccagctgga gcacatcctg gccgacaact gcgccatgca
     3181 gctggtccgc gcgcccaagc agttcgacgt catcgtcacc gacaacctgt ttggcgatat
     3241 cctgtcggac gccgccgcca tgctgaccgg ctcgctgggc atgctgccct cggcggcctt
     3301 gggcgcggcc ggcaagcccg gcctgtacga gcccatccac ggctcggccc ccgacatcgc
     3361 cggccagggc gtcgccaatc cgctggccgc catcctgtcg ttcgagatgg ccctgcgctg
     3421 gtcgctgggc cgcgccgagg cggccgacgt tctgctggcc gcggtcaagg cggccctgga
     3481 cggcggcgcg cgcacccgcg atctgggcgg ttcgctgtcg acggtggaaa tgggcgatgc
     3541 ggtgttggcc aggctttaag gtccggaaag ccctctccct gacgggggag gcgatggcct
     3601 ttggattcgc ccgcagagaa cgtgtcatcc cggacaagcc tggcgaagcc gg
//
LOCUS       DAOMEO010000191         3648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2911_length_3648_cov_3.301976, whole genome shotgun sequence.
ACCESSION   DAOMEO010000191 DAOMEO010000000
VERSION     DAOMEO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2911_length_3648_cov_3.301976"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLH31_05640"
     CDS             complement(<1..389)
                     /locus_tag="PLH31_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88744.1"
                     /translation="MTAQADSEIADPVRAEFDAAFYLAKNPDIAAAGVDPFEHFMTMG
                     WREGRDPNRRFSIASYLDVNPDIGEAGVNPFSHYVVTGRGEGRDLKLDLGFQYEILKS
                     AMPLEARIQALRAYFPDGEASPTEVLIS"
     gene            complement(540..2177)
                     /locus_tag="PLH31_05645"
     CDS             complement(540..2177)
                     /locus_tag="PLH31_05645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR88745.1"
                     /translation="MSKMNFAIYYLGDGYSTDKKIMGRQSAGKALMKGVARRWKTDEI
                     HGFGIGSATSGQAMLKQLQGDGFTGTLRWREAPGDKVLGELGAAYFPGPPTKEIAHAR
                     NSLNPAGYSFFGVTHTLSSAGAMDLVSDLVLPPFKPWDAMICTSSAALSVVTSLQDDF
                     KAWFAEHTGATRFNQIALPVIPLGVDAPAFARTPVQMAAARQALGLAGDDVAFLFAGR
                     MSFHAKANPTVFYQALEEACQRLGKPLVCIEAGVYPNPPMAEAFEKARQFLAPSARFI
                     QVDGQDEARYLEAWKAADVFVSLSDNIQETFGLTPLEAMAAGIPVLVSDWDGYKDTVR
                     DGIDGYRISVTLPPAGTGADLALSHALGQSTYDYYIGRVSMATAVDLTALTDRVIALA
                     QDPGLRRKLGQAGQNRIQQAYDWPIILDRYTDLARDLGEIRRGAGSSEQAIWPSRPDP
                     FHLFADYPTHTLTGSWQVIVHAGRSKAVDAFLELGVTRYAQHPTLLPRETILEVLHAA
                     ETQSCTVDSLLGDDERRPLRTRALMWLYKLGLVALRP"
     gene            complement(2883..>3648)
                     /locus_tag="PLH31_05650"
     CDS             complement(2883..>3648)
                     /locus_tag="PLH31_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HQR88746.1"
                     /translation="KRKRLKPIEYESPNNDVWVKVEAMPAYGMATIWDADILVWAAAV
                     LNHMKVQGVNDLPRTLKTTTYDLLRAIKRDTSGRGYQELQAALQRLETTSIRTSIRAP
                     KRRTEAQFGWLDGWTLEVDPESGQPRGMTITLSNWVYEGIVNERSLLTMHTDYFLLTG
                     GLERALYRIARKHAGNQRDGWTCRIEVLYEKTGSDSQPKEFNRMLRKVVERNQLPEYD
                     LSFTKTQDDSPALHVVHRDAVDINAIKAELAALNEE"
BASE COUNT          719 a         1060 c         1086 g          783 t
ORIGIN      
        1 gatatgagga cttccgtggg tgaggcctcg ccatcgggaa aataggcccg gagcgcctgg
       61 attctggcct ccagtggcat ggcggatttc aggatctcgt attggaaccc aagatcaagc
      121 ttgaggtccc tcccctcccc gcgccctgtc accacatagt gcgagaaagg gttgacgccg
      181 gcctcgccaa tgtccgggtt cacgtcgaga taactggcga tcgagaagcg acgattggga
      241 tcgcgtcctt cgcgccatcc catagtcatg aagtgctcaa aagggtcgac gcccgccgca
      301 gcgatatctg ggttcttggc cagatagaat gcggcatcga attcggcgcg aaccggatcc
      361 gcgatttcac tgtcagcttg ggccgtcatg acgtcctttt ttcgcccggc gaggagcgaa
      421 ggtttcgatc ggaaaggacc gaaacgatct agaaaccgga atttagcggc cggcgcttat
      481 acactgccag gccctcaatt cccttggcgc ggatagcgcg tcaagtcctg agcataccgt
      541 cagggcctga gcgctacgag acccagcttg tagagccaca tcaaggcgcg ggttcgtaga
      601 ggacggcgtt cgtcgtcgcc tagaaggcta tcaaccgtac agctctgcgt ttcagcggcg
      661 tgtaggacct caaggatcgt ctcgcgcggc agtagcgtcg ggtgttgggc gtaacgcgtc
      721 acaccgagct ccagaaaggc gtcaaccgcc ttggatctgc cagcatgcac aatcacctgc
      781 caggatcctg tcagggtgtg cgtcggatag tctgcaaaca gatggaaagg atcgggccga
      841 cttggccaga tggcctgttc cgaagagccc gcgcctcgcc ggatttcgcc caagtctctg
      901 gccagatcgg tatagcggtc cagaatgata ggccaatcat aggcttgctg gatacggttc
      961 tgtccggctt ggccaagctt gcgcctaagg ccaggatctt gagccagggc gatcacacga
     1021 tccgtcagcg ctgtcaggtc caccgctgtg gccatgctga cgcggccgat ataataatca
     1081 tacgtgcttt gccccagcgc atggctcagg gcgagatcgg cgccggttcc tgctggtggt
     1141 agggtgacgg atatgcggta gccatcgatc ccgtcgcgca ccgtatcctt gtagccgtcc
     1201 cagtcgctga ccagcacagg aatacccgcc gccatggctt caagcggggt cagtccgaag
     1261 gtttcttgga tattgtcgga cagagaaacg aaaacatccg ccgccttcca agcttcaaga
     1321 tagcgcgctt cgtcctggcc atcgacctga atgaaacggg cgctgggggc cagaaactga
     1381 cgggctttct cgaaggcctc ggccatcggc ggattgggat acacacccgc ctctatgcac
     1441 accaagggtt tgccgagccg ttggcaggcc tcttccaggg cttgatagaa caccgtcgga
     1501 ttggccttgg cgtgaaagct catgcgaccg gcaaacagaa aagccacatc atcgccagca
     1561 agacctagag cttgcctggc cgctgccatc tgcacgggcg ttcttgcgaa agccggcgca
     1621 tccaccccga gtgggattac tggcagggcg atctgattga agcgcgtggc tcccgtatgc
     1681 tcggcaaacc aggctttgaa atcatcctgg aggctagtca ccacagacag agccgcactg
     1741 gatgtacaga tcatcgcatc ccagggcttg aaaggcggca gaacaaggtc cgacaccaga
     1801 tccatcgcgc ccgctgacga cagggtgtgt gtgacgccga agaagctgta gccggcagga
     1861 tttaggctgt ttcgggcgtg ggcgatttcc ttggttgggg ggcctgggaa ataggccgcg
     1921 ccaagttctc ccagcacctt gtcgccaggc gcttctcgcc aacggagggt tccagtgaat
     1981 ccgtcgccct ggagttgctt gagcatggcc tggccagagg tggctgaccc tatgccgaaa
     2041 ccgtgaattt cgtcggtttt ccagcggcgc gccaccccct tcatcaaggc tttgcccgcg
     2101 ctttgacggc ccatgatctt cttgtcggtc gagtagccgt cgccaaggta atagatcgcg
     2161 aagttcatct tgctcatgga aacactcgcg actctggtgt gtgtggcgct gggcctcagc
     2221 ctctacctgt cagcgtctgg aaggaaaagt cgtggctagc gcaatggcgc tgctcaagtc
     2281 cacacaccgt gtctttcagg attgaaacca ggcttcacac cgtctgagcg tacgctttct
     2341 gacacccacc cttgtcgaga gccccggttg ggccctctca aggcccaaaa ccggggtgac
     2401 ggctctgacg ttcagtctcc gggcccgtta agagactctg ttagatacaa gttaaataag
     2461 ttaagcggca aaatagggct gttttatcaa ataatatcat aggcttgccg aacaccccgg
     2521 tgtgtgatcc cacgtgcttt ggtgtgtgat cccacgatga aaggtgtgtg atcccacgaa
     2581 gaaaaggtgt gtgatcccac gtcatctgaa ggcgacagag ccgacttatc cacagctctg
     2641 tggacaaact gagcggtgtg tgatcccacg aaggtcgccc gtttagcgaa tcaaacccgc
     2701 ctttgtttac gatcctcggc cttgtctggc tcaacaccgc gcgatcatcg gatctgccct
     2761 cctcgttact gtccagaagc gtcaagccct ggcgtacgcg gcggcaaagc ggtgtgtgat
     2821 cccacgatgg atcccctgcc ttctcccgac ttttcctatt gaaaaggttg ggctttgtat
     2881 ttctattcct cgttcagggc tgcaagttca gccttgatcg cgttgatgtc caccgcatcg
     2941 cgatgaacga cgtgtagagc cggtgaatca tcctgggtct tggtgaagga caggtcgtat
     3001 tccggcagtt ggttgcgttc gaccaccttg cgcagcatcc gattgaattc cttgggctgg
     3061 ctgtcgctgc cggtcttttc gtagagaacc tcgatccgac aggtccagcc gtcgcgctga
     3121 ttaccggcgt gcttgcgggc gatccggtaa agcgctcgct ccagtccgcc tgtcagcagg
     3181 aaatagtcag tgtgcatagt cagaagggac cgctcattga cgatgccctc atagacccaa
     3241 ttggacaggg tgatggtcat gccgcgcggc tggccgctct cgggatcgac ctcaagggtc
     3301 cagccgtcca gccagccgaa ctgggcttcg gtacggcgct tgggcgcccg gatcgaggta
     3361 cggatcgagg tggtttctag ccgctgcagg gcggcctgca gttcctgata gccccgaccg
     3421 ctggtgtcgc gcttgatagc gcgcagcagg tcataggtcg tggttttcag ggtgcgcggc
     3481 aggtcgttca ccccctgtac cttcatgtgg ttgagcaccg cagcggccca gaccaggatg
     3541 tcggcatccc agatggtcgc catgccgtaa gcgggcatcg cctcgacctt aacccagacg
     3601 tcgttgttgg ggctctcgta ctcaatcggc ttgaggcgct tgcgtttt
//
LOCUS       DAOMEO010000192         3644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2917_length_3644_cov_3.100585, whole genome shotgun sequence.
ACCESSION   DAOMEO010000192 DAOMEO010000000
VERSION     DAOMEO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2917_length_3644_cov_3.100585"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..423)
                     /locus_tag="PLH31_05655"
     CDS             complement(52..423)
                     /locus_tag="PLH31_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQR88747.1"
                     /translation="MQLFRSRKINRLAFVVFVAASLFTLWRAVLPDDGGVGFIPWDKA
                     KHFIAFYVLTALAVAALPNSRFWRIGAVLLAFGIGIEVVQALVGRDAAFGDVVADACG
                     IGALFGPIVLRSMTKRAPVAA"
     gene            complement(497..>1014)
                     /locus_tag="PLH31_05660"
     CDS             complement(497..>1014)
                     /locus_tag="PLH31_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQR88748.1"
                     /translation="EGEVSKLNVKPMTSAALAGILKPMLAEINMVSAPAVAKERGITV
                     SESRQEVSPTYDSLMRITITTEKGKRAFAGTVIAGAPRIVEVKGMELDAGFSPAMLYI
                     NNLDKPGFIGALGMLLGEAGVNIATFNLGRLSADEDAIALVGVDQAPNPELLGKIQAL
                     PHVKEARALTF"
     assembly_gap    1015..1024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1025..2114)
                     /gene="serA"
                     /locus_tag="PLH31_05665"
     CDS             complement(<1025..2114)
                     /gene="serA"
                     /locus_tag="PLH31_05665"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQR88749.1"
                     /translation="MTAPRVLIADSLSPAAVAIFTARGVQADVKTGLTKAELLEIIGD
                     YDGLAVRSATKADPDVIAAAKKLKVIGRAGIGVDNVNIPAATAAGIVVMNTPFGNSIT
                     TAEHAIAMMFALARQLPAADVSTQAGKWEKNRFMGVELYAKTLGLIGAGNIGSIVADR
                     ALGLKMKVVAFDPFLSPERAIEIGVEKVELDELLARADVITLHTPLTDKTRNILSAEA
                     LAKTKKGVLIVNCARGGLVDENALRALLDSGHIGGAGFDVFTEEPAKANVLFGSDKVV
                     ATPHLGASTNEAQENVALQVAEQMSDYLLTGAVTNALNSPSISAEEAPKLKPFVALAE
                     KIGALAGQMVDFGIAAIDKACAGAADIMR"
     gene            complement(2241..3413)
                     /locus_tag="PLH31_05670"
     CDS             complement(2241..3413)
                     /locus_tag="PLH31_05670"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HQR88750.1"
                     /translation="MTTLAKPAQRPARPEFSSGPCAKRPGWTPENLTNAVLGRSHRSK
                     LGKARLKAAIDQTREVLEVPADFLIGIVPGSDTGAVEMAMWSMLGAKPVQLMAFESFG
                     KDWVTDVTKQLKLPNVEVLDAPYGQLPDTSRVDPAKDLVFTWNGTTSGVRVPNADFIS
                     ADREGITICDATSAAFAQDLDWTKLDVVTFSWQKALGGEGAHGVLILSPRAVARLESY
                     TPAWPMPKLFRMTKGGKVSLDIFEGATINTPSMLCVEDALDALKWASSIGGLEGMQAR
                     ADQNLAVIADWVAKTPWVDFLAATPDIRSNTSVCLKVVDPAIVALPDDAQADFAKKLA
                     SLLEKEGAALDIGGYRDAPAGLRIWCGATVEASDLEALTPWLDWAFETVCAERVAA"
BASE COUNT          598 a         1119 c         1295 g          622 t
ORIGIN      
        1 tttagagcat ttttcgatcc gataaccgtt tcacacttat cggaaaatgc tctaggccgc
       61 gacgggggct cgcttggtca tcgagcgcag gacgataggc ccaaacagcg cgccgatccc
      121 gcaggcgtcg gcgacgacat cgccgaacgc ggcgtcacgg ccgaccaggg cctgcaccac
      181 ctcgataccg atgccaaagg ccagcaggac cgcgccgatc cgccagaaac ggctgtttgg
      241 cagggccgcg accgccaggg cggtcagcac atagaaggcg atgaagtgct tggccttgtc
      301 ccaggggatg aaaccaaccc cgccgtcatc cggcagcact gcgcgccaca gggtgaacag
      361 cgacgccgcg acgaagacga cgaaggccag ccgattgatc ttgcgactac ggaacagctg
      421 catgcctgac cttgaaccaa cgcgtcaccc caggaacagc gagtatgccg ccccggggtg
      481 acgaaatcgc aaaagcctag aacgtcagcg cgcgggcttc cttgacgtgc ggcagagcct
      541 ggatcttgcc cagcagctcg gggttcggcg cctggtcgac gccgaccagg gcgatggcgt
      601 cttcgtcggc cgacaggcgg cccaggttga aggtggcgat attgacgccc gcctcgccca
      661 gcagcatgcc cagggcgccg atgaagcccg gcttgtcgag gttgttgata tagagcatgg
      721 ccggcgagaa gcccgcgtcc agctccatgc ccttgacctc gacgatgcgc ggcgcgccgg
      781 cgatcaccgt gccggcaaag gcgcgcttgc ccttttcggt ggtgatcgtg atgcgcatca
      841 ggctgtcata ggtggggctg acctcctggc ggctttcgga gacggtgatg ccgcgctcct
      901 tggccacggc cggggcggag accatgttga tctcggccag catcggcttg aggattccgg
      961 ccagggccgc cgaggtcatc ggcttgacgt tcagcttgga gacctcgccc tcatnnnnnn
     1021 nnnnaacgca tgatgtcggc ggcgccggcg caggccttgt cgatggcggc gatgccgaag
     1081 tcgaccatct ggccggccag ggcgccgatc ttctcggcca gggccacgaa gggcttcagc
     1141 ttgggggcct cttcagcgct gatcgagggg ctgttgaggg cgttggtcac ggcgccggtc
     1201 agcaggtaat cgctcatttg ctcggcgacc tgcagggcaa cgttttcctg ggcttcgttg
     1261 gtgctggcgc ccaggtgcgg ggtggccacg accttgtccg aaccgaacag cacattggcc
     1321 ttggccggct cttcggtgaa cacgtcaaag cccgcgccgc cgatgtggcc gctgtcgagc
     1381 agcgcgcgca gggcgttctc gtcgaccagg ccgccgcggg cgcagttgac gatcagcacg
     1441 cccttcttgg tcttggccag ggcctcggcc gacaggatgt tgcgggtctt gtcggtcagg
     1501 ggcgtatgca gggtgatgac gtcggcgcgg gccagcaact cgtccagctc caccttctcg
     1561 acgccgatct cgatggcgcg ttcggggctg aggaacgggt cgaaggccac gaccttcatc
     1621 ttcaggccga gagcccggtc ggcgacgatg gagccgatat tgccggcccc gatcaggccc
     1681 agggtcttgg cgtacagctc cacgcccatg aagcggttct tctcccactt gccggcctgg
     1741 gtcgagacgt cggcagcggg cagctggcgg gccagggcga acatcatggc gatggcgtgc
     1801 tcggccgtgg tgatcgagtt gccgaacggg gtgttcatca cgacgatgcc ggcggcggtg
     1861 gcggccggga tgttgacatt gtcaacgccg atgccggccc ggccgatgac cttcagcttc
     1921 ttggcggcgg cgatgacgtc cgggtcggcc ttggtggccg agcggacggc caggccgtca
     1981 tagtcgccga tgatttcgag gagttcggcc ttggtcaggc cggtcttgac gtcggcctgg
     2041 acgccgcggg ccgtgaagat ggcgacggcg gcgggggaca gggagtctgc gatgaggacg
     2101 cggggagcgg tcatggggat gtccaatctg ggagagccgt cctgggtctt cgacgaaccg
     2161 aggatcaggc tgctgaggtg tgggagccgg gccctcgtcc tggcgtatcg cgggacgagg
     2221 gctcggtcgc agggcgtggg ttaggcggcg acccgttcgg cgcagacggt ctcgaacgcc
     2281 cagtcgagcc agggcgtcag ggcttccaga tccgaagcct cgaccgtggc gccgcaccag
     2341 atgcgcaggc ccgccggggc gtcgcgatag ccgccgatgt cgagggccgc gccctccttc
     2401 tcaagcaggc tggccagctt cttggcgaag tcggcctggg cgtcgtcggg cagggcgacg
     2461 atcgcaggat cgacgacctt gaggcacacc gaggtgttgg accggatgtc cggcgtggcg
     2521 gccaggaaat cgacccacgg ggtcttggcc acccagtcgg cgatgaccgc caggttctgg
     2581 tcggcgcggg cctgcatgcc ttccagaccg ccgatggacg aagcccactt cagggcgtcg
     2641 agcgcgtctt ccacgcagag catcgacggg gtgttgatcg tggcgccctc gaagatgtcg
     2701 agggaaacct tgccgccctt ggtcatacgg aagagcttgg gcatcggcca ggccggcgtg
     2761 tagctctcaa ggcgcgccac ggcgcggggc gacaggatca gcacgccgtg cgcgccctcg
     2821 ccgccgagcg ccttctgcca ggagaaggtg acgacatcga gcttcgtcca gtccaggtcc
     2881 tgggcgaagg cggcgctggt ggcgtcgcag atggtgatgc cttcacggtc ggccgagatg
     2941 aagtcggcgt tcgggacgcg gacgcccgag gtggtgccgt tccaggtgaa gaccaggtcc
     3001 ttggccggat cgaccctgga cgtgtcgggc agttggccgt atggcgcgtc gagcacttcg
     3061 acattgggca gcttcaactg cttggtcacg tcggtgaccc agtccttgcc gaacgattcg
     3121 aaggccatca gttgcacggg cttggcgccc agcatcgacc acatggccat ttcgacggcg
     3181 ccggtgtcgg agccgggaac gatgccgatc aggaagtcgg ccggcacttc cagcacttcg
     3241 cgcgtctggt cgatagcggc cttgaggcgc gccttgccca gcttggagcg gtgcgaacga
     3301 cccagcacgg cgtttgtgag attttcgggg gtccagccgg gccttttggc gcacgggccg
     3361 gaagagaact cgggacgagc cgggcgttgc gccggcttgg cgagggtagt catagcgatg
     3421 tctcccatcc ttacagatgg acagcgcccc gttggggggg cgtggcccgc cggcgagaaa
     3481 cgccgtttca ggccgaaagg caaggagaga tttgtcgcgg acgcgggttt ttaggaccgc
     3541 cccagcaatt attttgcggc gcgcatgcgc ttgcgccagg ccagcagggt tccgatcagg
     3601 atgcaaagca acacacccag cgagtcgaga ttattgatca tcgc
//
LOCUS       DAOMEO010000193         3638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2920_length_3638_cov_2.377337, whole genome shotgun sequence.
ACCESSION   DAOMEO010000193 DAOMEO010000000
VERSION     DAOMEO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_3638_cov_2.377337"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..564
                     /locus_tag="PLH31_05675"
     CDS             274..564
                     /locus_tag="PLH31_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88751.1"
                     /translation="MTLDDLDAMLEHLVVAQAASGDPEDGPGLITLSAETRGGFGLGS
                     GMECTNILKGIRYRGLRVIVTRAMEDAVFSRKEARAKGHDAFDDFEPSPPRG"
     gene            964..>2377
                     /locus_tag="PLH31_05680"
     CDS             964..>2377
                     /locus_tag="PLH31_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HQR88752.1"
                     /translation="MTVHAQIAADPAAHNAAQASALLADAQTEQLIPLNRLKKSLRNV
                     RKVKHIDADIEAFAASLKAKGLLQNLVVEPEIRDGVATGYYLVTAGEGRRLGLKRLAK
                     RRQIARDHLVRCVVDTRNDPGEVSLDENTSRRSMHPADEFEAFADLADRKGWGAVEIA
                     ARFGVEPEFVTKRLRLGAVAPALLEVYREEGLTLEQLMAFAVNPDTERQMQVFAQLPE
                     HNRQTYAIRRAMTETKIAADDRRAVFVGMEAYAAAGGAVLRDLFTEGPGWLEDVALLE
                     RLVVEKLEGLATLARDEEGWKWSAAYLDHPHGHGCRRVWEHPLARSPEVQARIDALTA
                     ERDALVIRIGVADETPDSADARFEAVESELADLADDYGYEPEEKARAGLFVVLSAYGE
                     PRIERGFVRPEDEPAPEDEGDEGADDGAAGEADDLTSDGGDPGDDGADDLPDTEEEPA
                     GDPRAPLPARLVADLTAHKTA"
     assembly_gap    2378..2387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2388..3033
                     /locus_tag="PLH31_05685"
     CDS             <2388..3033
                     /locus_tag="PLH31_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR88753.1"
                     /translation="ALRDLVGQSPDMALIALTHGLVLQAFGYRFPLASCLDLQIQSRD
                     LRGDGDGIEAGRAGQALAERHAGWARQLPAEASEVWAFVLGLDHDSRMSLLAHCVSLS
                     VNGVHGVERKPAAWLHAERLAGALDLDMRDYWSPTAERYLGRVTKARVLAAVEEAVSP
                     EAAGRLTGLKKDALVDAAQPSLVEARWLPRLLRTERSEAGAAEAAPESEARGAS"
     gene            complement(3030..3257)
                     /locus_tag="PLH31_05690"
     CDS             complement(3030..3257)
                     /locus_tag="PLH31_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88754.1"
                     /translation="MNVQMKDITVTSREGRVYHVDQVYIRGSHVRFFIIPDMLRNAPM
                     FRARNVRGRGVGLARGRATVSRARATGRGGR"
BASE COUNT          568 a         1177 c         1329 g          554 t
ORIGIN      
        1 gcccgcgtgc ggatgcgtga cggcgtccag gcgacgcagc accttgaagc ggccgctggc
       61 ctccagcgtc cgcgccatgg cttccagatc gtcttgcgcc gtcaagcggc ctggcggggc
      121 ggaatcgtcg ggcatcgcgg acctcctggc tttagttggt agtccatcgg cggtcgacga
      181 ggccagaacc aagcgcgcga catcgatggg gataaccccg gcctttcgtc ggccggccgg
      241 ccgcgacgcg atgaatcggt ctaggccgac gccatgaccc tggatgatct ggacgccatg
      301 ctcgaacacc ttgtcgtcgc gcaagcggcg tcgggcgatc cggaggacgg ccctggcctg
      361 atcacgctgt cggccgaaac ccgcggcggg tttggcctcg gttccggcat ggaatgcacc
      421 aatatcctca agggcatccg ctatcgcggt cttcgggtga tcgtgacccg cgccatggaa
      481 gacgccgtct tctcgcgcaa ggaagcccgc gccaagggcc atgacgcctt cgacgatttc
      541 gagccgtcgc cgccgcgcgg ctgaccttgg tgtcggagag cggccggcga tcacgctcgg
      601 tcccgccgcg ccttcgcctt gctgcggcgc ggctcctgcg ggcgtgacgc ctgcgggcgc
      661 ctttcgggcg ccgccggccg ctcgcgccgc gtcccgaagc cgctttgcgg cttctccttg
      721 cagtctcctc ttttgcgaag aggcgggcgc tttgcgcccg cgggcttagg gctccgcccc
      781 cagcgcgctt cggaccggct tccgcccagc ttcgggcgcg cgggctagac gggcagggcg
      841 ggcagcaacg ccctgccagc agggtccccg cgaagccggc cgctccgctt cacccccggt
      901 ctcgccgacg ggcaagggat caggcgcgcc ttgcgcctga tcccctagcc caaggaggca
      961 gacatgactg ttcacgccca gatcgcggcc gacccggccg ctcacaacgc cgctcaagct
     1021 tcggccctgc tggccgacgc ccagaccgag cagctgatcc cgctcaacag gctcaagaag
     1081 agcttgcgca atgtccgcaa ggtcaagcac atcgacgccg acatcgaggc cttcgcggcc
     1141 agtctcaagg ccaagggcct gttgcagaac ctcgtggtcg agcccgaaat ccgcgacggt
     1201 gtcgccaccg gctactatct cgtcacggcg ggcgaaggcc gccgtctggg gctcaagcgg
     1261 ctggccaagc gtcggcagat cgccagggac caccttgtgc gctgcgtggt ggatacacgt
     1321 aacgaccccg gcgaggtgtc cttggatgaa aacaccagcc gccgcagcat gcatccggcc
     1381 gacgaattcg aggcgttcgc cgatcttgcc gaccgcaagg gctggggcgc cgtcgagatc
     1441 gccgcgcggt tcggcgtcga gccagagttt gtcaccaagc gtctgcggct aggcgccgtc
     1501 gctccggcct tgctggaagt ctatcgcgag gagggcctga cgctggagca gctgatggcc
     1561 ttcgcggtca atcccgacac cgagcgccag atgcaggtct tcgcgcagtt gccggaacac
     1621 aaccgccaga cctacgccat ccgccgcgcc atgaccgaga ccaagatcgc ggccgacgac
     1681 cgccgggccg tgttcgtcgg catggaggcc tatgcggcgg cgggcggcgc ggtgctgcgc
     1741 gacctcttca ccgaggggcc gggctggctg gaggacgtcg ccctgctgga gcgtctggtc
     1801 gtcgaaaagc tcgaaggcct cgccactttg gcgcgcgacg aggagggctg gaaatggtcg
     1861 gcggcctatc tcgatcaccc gcatggccat gggtgccgcc gcgtttggga acatcctctg
     1921 gcccggtcgc ccgaggttca ggcccggatc gacgccctga ccgccgagcg cgacgccttg
     1981 gtgatccgga tcggcgtggc ggacgagacg cctgacagcg ccgatgcgcg gttcgaggcc
     2041 gtcgagagcg agctggccga cctcgccgac gactacggct acgagcccga ggaaaaggcg
     2101 cgcgcgggcc ttttcgtggt gctcagcgcc tatggcgagc cgcgtatcga acgcgggttc
     2161 gtgcggcccg aggacgagcc cgcgccggag gatgagggcg acgagggggc cgacgacggg
     2221 gcggcgggcg aggcggatga tctcaccagt gacggcggcg atcccggcga cgacggcgcg
     2281 gacgacctgc ccgacaccga ggaagaaccg gcgggcgatc ccagggcgcc gttgcccgcg
     2341 cggctcgtcg ccgacctcac cgcgcacaag accgcagnnn nnnnnnnagc cttgcgcgat
     2401 ctcgtcggcc agtcgcccga catggcgctg atcgccttga cccatgggct ggtgctgcaa
     2461 gccttcggct atcgctttcc tctggcctcc tgcctcgatc ttcagattca gtcgcgggac
     2521 ctgaggggcg atggcgatgg gatcgaggct ggccgggcgg ggcaggccct ggccgaacgt
     2581 cacgcgggct gggcgcggca attgcccgcc gaggcgagcg aggtgtgggc gttcgtcctg
     2641 ggtctcgacc acgacagtcg catgagcctt ctggcccact gcgtgagctt gtcggtcaac
     2701 ggcgtgcatg gcgtcgaacg caaacccgcc gcgtggctgc acgccgagcg gctggccgga
     2761 gcgctcgatc tggacatgcg cgactactgg agcccgaccg ctgagcgcta cttgggccgg
     2821 gtcaccaagg cgagggtgct ggcggcggtc gaggaggcgg tctcgcccga ggcggcgggg
     2881 cggctgacgg ggctcaagaa ggacgccctc gtcgacgcgg cccagccttc gctggtcgag
     2941 gcgcggtggt tgccccgctt gctgcgcacc gagcgttccg aagccggggc ggcggaggcg
     3001 gcgcccgaat cagaggcgcg gggggcatcc tagcgaccgc cgcggccggt ggcacgggcg
     3061 cgactgacgg tggctcgtcc acgggcgaga ccgacgccgc ggccgcggac gttgcgggcg
     3121 cggaacatgg gcgcgttgcg gagcatgtcg gggatgatga agaagcggac gtgcgagccg
     3181 cggatgtaga cctggtcgac gtggtagacg cggccctcgc gcgaggtgac ggtgatgtcc
     3241 ttcatctgga cgttcatgga gtcctcggct gtttgcgatg aacgggcgga cagttagcgg
     3301 attattctcc caagatcaaa cgagcgcatg atggaaggaa tgggaggaag ggaaagaggt
     3361 tggggcggaa gacttgccat cgaggagggt gccgcggtag gagttgccgt tggtaagctc
     3421 gaccgtcacg atgtggccct gcagcgcgga aagggagaaa ggttagcagc tgccaaacac
     3481 gcgcgcgttg ggggaggtgt tcgggaagga ggcaatgcaa cgaaccgtgg cctcgtggag
     3541 gagcttgatc gggatgccaa tgttggacgt catggtcgcg ggcgggagtt gcagctgccg
     3601 ttggcttcga agcgcgagca gggaaggaga gaggcttg
//
LOCUS       DAOMEO010000194         3628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2925_length_3628_cov_2.172964, whole genome shotgun sequence.
ACCESSION   DAOMEO010000194 DAOMEO010000000
VERSION     DAOMEO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2925_length_3628_cov_2.172964"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>366
                     /locus_tag="PLH31_05695"
     CDS             <1..>366
                     /locus_tag="PLH31_05695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR88755.1"
                     /translation="LARDIGVIRFDERNTGASTGDHKTARSADLQNDIASILDAAAKV
                     PGVDPKRIYLFGHSEGAVLAERLSIERKDIAGIVLIGAPGKSGRLMTLDQNRVVTPRT
                     PGMSDADFEKAVDAAQAKEV"
     assembly_gap    367..376
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <377..697
                     /locus_tag="PLH31_05700"
     CDS             <377..697
                     /locus_tag="PLH31_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR88756.1"
                     /translation="VEFLKTRPSLNDLLELDALGLGRQVKVPALILEGAEDWQVQPPQ
                     GERLAKAMTEAGNREVTYRRLPRVGHLMTDNPPGVTDYDKLTDYAVAPSVLEAVTTWV
                     SAHN"
     assembly_gap    819..828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<829..1291)
                     /locus_tag="PLH31_05705"
     CDS             complement(<829..1291)
                     /locus_tag="PLH31_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQR88757.1"
                     /translation="MTLNSTTRTFALGLAMTASALTLVSAPAAFAVEQKMGGVIGCNA
                     AGSKQEVGAVIGDLLGAVAGNNLAKNERGAGTAIGAVAGAGAGSYIGCKMQKKDARQA
                     VGGAYKYDGVRFANSVQAQPVTRINGKYVARSNLNLRASASTRGAKVGGVGA"
     gene            complement(1445..1867)
                     /locus_tag="PLH31_05710"
     CDS             complement(1445..1867)
                     /locus_tag="PLH31_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88758.1"
                     /translation="MFAAVPLLALPVLVYNLIVLTLSGAFKSAEAHARITETLFTIHM
                     TSRVDWAVSLGDLLLAASLVVLFVELLKSTTSRKIAIINHSLSLVLFIICLIEFLLAP
                     AFATSTFFLITVMVLLDVLAGFIVTIVAARRDVDFGDN"
     gene            complement(1966..2703)
                     /locus_tag="PLH31_05715"
     CDS             complement(1966..2703)
                     /locus_tag="PLH31_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR88759.1"
                     /translation="MTADAAKVPDYSIVVPVFDEGGAAPALAREIAAAFAGENHEILF
                     IDDASRDDTRALLVALKAEIPQLRVLSHRKNSGQSRAVRSGVLAARGPIIVTLDGDGQ
                     NDPADAPRLARALAAGPETLALVGGERVKRQDSSAKRFASKFGNGVRKRLLKDTANDT
                     GCGLKAFRREAFLRLPYFDHIHRYIPALMLREGYEVAFEPVNHRHRETGVSKYTNFGR
                     LKASVSDLLGVMWLQTRLRNPQGVDEA"
     gene            2760..>3628
                     /locus_tag="PLH31_05720"
     CDS             2760..>3628
                     /locus_tag="PLH31_05720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR88760.1"
                     /translation="MTLESRLDAWSRGWRGPLFAALVALLAGLPGLIAMPPLDRDESR
                     FAQATAQMLETDDMVVIKFQDQPRFKKPVGIHWLQAATVSLVSAAEDRGIWAYRIPSL
                     LGAMLAAAACAWGAAALFDPRTGLLAGAILGSTFLLSSEAFIAKTDAVLCGTTTLAMA
                     ALAKMYADHLAGRTPNHWTKLAFWGGLAVAALIKGPVGLLTVIFAAVALWAWDRKAPW
                     LKTLGWSWGLVLFAAVVLPWAMMITVATDGAFWGVAIAGDLAPKMVGGQEGHSGPLGY
                     HALLTPLLIYPATL"
BASE COUNT          600 a         1182 c         1261 g          565 t
ORIGIN      
        1 gctggcccgc gacatcggcg tcatccgatt cgacgaacgc aacaccggcg cctccaccgg
       61 cgaccacaag accgcccgct cggcggacct gcagaacgac atcgccagca tcctggacgc
      121 cgccgccaag gtcccgggcg tcgatccaaa gcgcatctat ctcttcggcc acagcgaagg
      181 ggcggtgctg gccgagcggt tgtcgatcga gcgcaaggac atcgccggga tcgtgctgat
      241 cggcgcgccg ggaaaatcgg gccgcctgat gaccctcgac cagaaccgcg tcgtcacccc
      301 gcgtacgccg ggcatgagcg atgccgattt cgaaaaggct gtcgatgcgg cccaggccaa
      361 ggaggtnnnn nnnnnngtcg aattcctcaa gacccggccg tcgctgaacg acctgctcga
      421 actggacgcc ctgggcctgg gccgtcaggt caaggttccg gcgctgatcc tggaaggcgc
      481 cgaagactgg caggtgcagc cgccgcaggg cgagaggctg gccaaggcta tgacggaggc
      541 gggcaacagg gaggtcacct accgcaggct gccgcgcgtc ggccacctga tgaccgacaa
      601 tccgcccggc gttaccgact atgacaagct gacggactac gccgtcgcgc cgtcggtgct
      661 ggaggcggta acaacctggg tgtcggccca caactagccc ctctccgagc cccccccgga
      721 ctgaacaaag taaagcccgc ctggtcgggg gaacgaccag acgggctcgt gcatggaacg
      781 cggggaggga cggaaccgcg ttccagacct ctatgactnn nnnnnnnncg gcgccgacgc
      841 cgcccacctt ggcgccgcgg gtcgaagccg aggcgcgcag gttcaggttc gagcgggcga
      901 catacttgcc attgatcctg gtcacgggct gggcctgaac cgagttggcg aagcgcacgc
      961 cgtcatactt gtaggcgccg ccgacggcct ggcgggcgtc cttcttctgc atcttgcagc
     1021 cgatgtagga gccggcgccg gcgccggcga ccgcgccgat ggccgtgccc gcgccgcgtt
     1081 cgttcttggc cagattgtta ccggcgacgg cgcccagaag gtcgccgatc acggcgccga
     1141 cttcctgctt ggaaccggcg gcgttgcagc cgatgacgcc gcccatcttc tgctcgacgg
     1201 cgaaggcggc cggggccgaa accagggtca gggcgctggc ggtcatggcc aggcccaggg
     1261 cgaatgtccg ggtggtgctg ttgagggtca tggtgagtat ccttcgtccc gtgaatgctt
     1321 ccggggtcat caaccccgtg agacagacaa tgacaggacg aagctgaacg gggtcgcaga
     1381 ccgcccggac agaaatcgtt catgtgcaga aaaggtctgg gcggggcggg cctcgccctt
     1441 cggactagtt gtcgccgaag tccacgtcgc ggcgcgcggc gacgatggtg acgataaagc
     1501 cggccagcac gtccagcagc accatcacgg tgatcaggaa gaaggtcgag gtggcgaagg
     1561 ccggggccag caggaactcg atcaggcaga tgatgaacag caccagcgac agcgagtgat
     1621 tgatgatcgc gatcttgcgg ctggtggtgg acttcagcag ctcgacgaac agcaccacca
     1681 gcgacgcggc cagcaacaga tcgcccaggc tgaccgccca gtcgacgcgt gaggtcatgt
     1741 ggatggtgaa cagggtctcg gtgatgcgcg cgtgcgcctc ggccgacttg aaggcccccg
     1801 acagggtcag gacaatcaga ttgtagacca gcactggcag ggccagcagc ggaaccgcgg
     1861 cgaacatcga cagcctctcc cccaaggacg cgcggccgtc agggtcgcgc cgctaacggt
     1921 taaccggatt ttccgaaggc gagaaggcgg ttcaactcct aagagctaag cttcgtccac
     1981 gccctgagga ttgcgcagtc gcgtctgcag ccacatcacg cccagaaggt cgctgaccga
     2041 ggccttgagc cggccgaaat tggtgtattt cgacacgccg gtttcgcggt ggcgatggtt
     2101 caccggctcg aacgccacct catagccctc gcgcagcatc agggccggga tatagcggtg
     2161 gatgtggtcg aaatagggaa gccgcaggaa ggcctcgcgg cggaaggcct tcaggccgca
     2221 gccggtgtcg ttggccgtgt ccttgagcag ccgcttgcgc acgccgttgc cgaacttgga
     2281 agcaaagcgc ttggccgagc tgtcctggcg cttgacccgt tcgccgccga ccagggccag
     2341 ggtctcgggg ccggccgcca gggcgcgggc caggcgcggg gcgtcggccg ggtcgttctg
     2401 gccgtcgccg tccagggtga cgatgatcgg gccgcgcgcc gccagcacgc cgctgcgcac
     2461 ggcgcggctc tggccggaat tcttgcggtg gctcagcacg cgcagctgcg ggatctcggc
     2521 cttcagggcc accagcaggg cgcgggtgtc gtcgcggctg gcgtcgtcga tgaagaggat
     2581 ctcgtggttt tcgccggcga aggcggcggc gatctcgcgc gccagggccg gggccgcgcc
     2641 gccctcgtca aagacgggaa cgacgatcga atagtcggga actttggcgg cgtcggcggt
     2701 catcgccctc taatacctga aaactcccca cgcgaaagga tcgtgctatt ccatggcgca
     2761 tgacgcttga atctcgcctg gatgcctgga gccgcggctg gcgcggcccg ttgttcgccg
     2821 ccctggtggc gttgctggcc ggcctgccgg gcctgatcgc catgccgccg ctggaccgcg
     2881 acgagtcgcg cttcgcccag gccaccgccc agatgctgga aaccgacgac atggtcgtca
     2941 tcaagttcca ggaccagccg cgcttcaaga agccggtcgg catccactgg ctgcaggcgg
     3001 cgacggtgtc gctggtttcc gcggccgagg atcgcggcat ctgggcctat cgcatcccgt
     3061 ccctgctggg ggcgatgctg gcggcggcgg cctgcgcctg gggggcggcg gcgctgtttg
     3121 atccgcgcac gggcctgctg gccggcgcga tcctgggctc gaccttcctg ctgtccagcg
     3181 aggccttcat cgccaagacc gacgcggtcc tgtgcggaac cacgactttg gccatggccg
     3241 ccctggcgaa gatgtacgcc gaccacctgg cgggacgaac gcccaaccac tggaccaagc
     3301 tcgccttctg gggcggactg gccgtggcgg ccctgatcaa gggacctgtg ggcctgctga
     3361 cggtgatctt cgccgccgtg gccctctggg cctgggaccg caaggcgccc tggctgaaga
     3421 cactgggctg gagctggggg ctggtgctgt tcgccgccgt ggtcctgccc tgggcgatga
     3481 tgatcaccgt ggccactgac ggcgccttct ggggcgtggc catcgccggc gacctggcgc
     3541 ccaagatggt cggcggtcag gagggtcatt cggggccgct gggctatcac gccctgctga
     3601 ccccgctgtt gatctatccg gccaccct
//
LOCUS       DAOMEO010000195         3620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2937_length_3620_cov_2.806732, whole genome shotgun sequence.
ACCESSION   DAOMEO010000195 DAOMEO010000000
VERSION     DAOMEO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2937_length_3620_cov_2.806732"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLH31_05725"
     CDS             <1..543
                     /locus_tag="PLH31_05725"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQR88761.1"
                     /translation="VWWTFRAMGHEDVVVLDGGLPKWIAEGHPVEDGPAMPQERHFTP
                     RYQADIVRSVEQMKRNLETGREQVVDARPAGRFTGEAPEPRAGLRGGHIPGSRNIPLS
                     DMLAPDGTMLTADKLRVVFAAAGVDLAKPVAATCGSGITASVVALALARLGHPRAAVY
                     DGSWTEWGGLEDTPVVTGPA"
     gene            606..1722
                     /gene="metC"
                     /locus_tag="PLH31_05730"
                     /pseudo
     CDS             606..1722
                     /gene="metC"
                     /locus_tag="PLH31_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522185.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
     assembly_gap    1723..1732
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1733..2299
                     /locus_tag="PLH31_05735"
     CDS             <1733..2299
                     /locus_tag="PLH31_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HQR88762.1"
                     /translation="MTTHIALLRGVSVGGQKPIAAETFVDLLTELGFANARTVGGPSN
                     LVFDSPERTGADLQTFLETEIHTRLGLRTDIYVRTAQAWAAIIAANPYAEFAETDPGL
                     LMTLFLKDPPDKKVIGALRTKLTEGEQLRVESRQFYVTYPNGLGASKFSNNVVEKALA
                     TRITSRSWTIVQAIGEATAETDAPALED"
     gene            complement(2296..>3620)
                     /locus_tag="PLH31_05740"
     CDS             complement(2296..>3620)
                     /locus_tag="PLH31_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR88763.1"
                     /translation="RTFNALLGIDEATFMRETQSTFKLGISFENWGQIGDRYIHSFGQ
                     VGKSTWMGGFHHFWLQARAQGFGGELGDYCFELKAAEADRFFTDENATTNYAYHLDAT
                     LYGRFLRKMSEGKGVRRLEGKIARVQQRAEDGFIEALVMESGEIVEGDLFIDCTGFRG
                     LLIEQTLKAGWEDWSHWLPTNSALAVQTTSTEPAVPYTRAIAHKAGWRWKIPLQHRVG
                     NGLVYCSDHMSDDEARALLLREIDGEPLIEPRVIRYGAGRRRKTWSKNCVALGLASGF
                     VEPLESTSIHMIMIGVTRLMQLFPFGGVNDALIERYNRLAEDELEKIRDFIILHYKAT
                     ERSDSPFWDYCRTMDIPDSLAQRIDLFRENAQVFQAPGELFQVDSWLQVLLGQRVEPR
                     SHHHMGRMMPPDQLRQALDSLKGNIARAVEGMPSHQAFVDRYCAAWST"
BASE COUNT          616 a         1215 c         1154 g          625 t
ORIGIN      
        1 gtctggtgga ccttccgggc catgggccac gaggatgtgg tggtgctgga tggcggtttg
       61 cccaagtgga tcgctgaggg tcatcccgtc gaggacggtc ccgccatgcc gcaggaacgt
      121 cacttcaccc cccgctatca ggccgacatc gtccgttcgg tggagcagat gaagcgcaac
      181 ctcgagaccg gccgcgagca ggtggtcgac gcccgccccg ccggccggtt cacaggcgaa
      241 gcacccgaac cccgcgcagg cctgcgtggc ggccacatac cgggctcgcg caacattccg
      301 ctgagcgaca tgctggcgcc ggacggcacg atgttgaccg ccgacaagct gcgcgtggtc
      361 tttgccgctg ccggcgtgga tctcgccaag ccggtcgccg ccacctgtgg ctcgggcatc
      421 accgcctcgg tcgtcgccct ggccctggcc cgcctcggcc atccccgcgc cgccgtctat
      481 gatggctcat ggaccgaatg gggcggtctt gaggacaccc cggtcgtcac gggacccgcc
      541 tgacgcttgg cgcatcgggc gagccggcct aaaccacgac ttcgccacga cgccgagagc
      601 accttatgga tgaagaaacc cgcctgatcc gaacgggcgc atcgtggccc ctgccggcca
      661 agacggtcaa tccgccgatc cagcgcggct cgacggtgct gatgccaagt gcggcgtcgc
      721 tctatgacga tgaccagatc agctacggca tcactggcct cgcgtcgcct ggcgccttgc
      781 aggcggccct ggccgtgctg gaaggcgcaa cgaacgtgac cctctatccg tcaggcctgg
      841 ccgccatcac cggcgccctg cttgcggtgc tgaaggccgg cgacgagatc ctggtggttg
      901 atagcgccta caggcctaca cgccggttct gcgaccaggt gctggcccgc ttcggcgtgg
      961 caacgaccta ctatgacccg gccctatcgc cagacgacct gatggccctg accaacgcca
     1021 ggacccggct gatcctgctg gaggcgcccg gctcactcac cttcgagatg caggacgtcc
     1081 cggccatcgc cgcagcggcc aaggcgcgcg gcatcctcac cctgatcgac aatacctggg
     1141 cagcgggcct ctatttcaag ccgttggccc atggcgtgac gatcagcatc caggccctga
     1201 ccaaatatgt cggggggcat tcggactgtt tcatggggtc agtcgccacg ctggacgcag
     1261 cgatcggcaa gcagctggac gacgccatgt gggacatcgg ctggtcggtg tcgccggacg
     1321 acgcctacac catgcttcgc ggcgtgcgca cgatggcgac gcgcctgacc agaaatcacg
     1381 agaatggcct gaccatcgca cgctggatgc agtagcagtc cgaggtggcg cgggtgctgc
     1441 accccgcact gcctgacgat ccgggttacg ccctttggga gcgcgacttc accggcgcct
     1501 gcggtctgtt cggcgtggtg ctgaagcccg cgcgcgagaa ggccgtgcac gccttcctcg
     1561 acgccctgca gctgtttggc ctgggttttt cctggggcgg ctatgagagc ctggcgatcc
     1621 actgcgatcc gcagttgaac agccgcactt tccctgtcga cctggaaggc ccattgctgc
     1681 gcctgcacat cggcctggaa accccggccg acctcatcat cgnnnnnnnn nnatgaccac
     1741 tcacatcgcc ctgctgcgcg gcgtcagcgt cggcggccag aagccgattg cggcagagac
     1801 cttcgtcgac ctgctgaccg agttgggttt cgccaacgcc cgcacggtgg gagggcccag
     1861 caacctggtg ttcgacagcc ccgagcgcac cggcgccgac ctgcagacct ttctcgagac
     1921 cgagatccac acccgccttg gcctgcgcac cgacatctat gttcgcaccg cccaggcctg
     1981 ggcggcaatc atcgccgcca atccctatgc cgagttcgcg gaaaccgatc ccggcctgct
     2041 gatgaccctg tttctgaagg atccgcccga caagaaggtc atcggcgccc tgcgcaccaa
     2101 gttgaccgaa ggcgagcagc tgcgcgtcga aagccgccag ttctacgtca cctaccccaa
     2161 tgggctaggt gcgtcgaagt tcagcaacaa cgtcgtcgag aaggccctgg cgacccgcat
     2221 cacgagccgc tcctggacca tcgtccaggc catcggcgag gcgacggccg aaaccgacgc
     2281 cccggctttg gaagactagg ttgaccaggc cgcgcagtag cgatccacga aggcttgatg
     2341 gctgggcatg ccctcgacgg cccgggcgat attgcccttg agcgagtcca gagcctgacg
     2401 cagttggtcg ggcggcatca tgcggcccat gtggtggtgg ctgcgcggct cgaccctttg
     2461 gcccagcagc acctgtagcc aggaatccac ctggaacagc tcgcccggcg cctggaatac
     2521 ctgggcgttt tcgcggaaca ggtcgatgcg ctgggccagg ctgtcgggga tgtccatcgt
     2581 ccggcaatag tcccagaacg ggctgtcgct ccgctcggtg gccttgtagt gcaggatgat
     2641 gaagtcgcgg atcttctcca gttcatcctc ggccagacga ttgtagcgct cgatcagggc
     2701 atcgttgacg ccgccgaacg gaaacagctg catcagccgt gtgacgccga tcatgatcat
     2761 gtggatcgag gtggattcca gaggctccac aaagccgctg gccaatccca gggcgacaca
     2821 gttcttgctc caggtcttgc ggcgacgccc ggcgccatag cggatcacac ggggctcgat
     2881 caggggctcg ccgtcgatct cgcgcagcag cagggcgcgg gcctcgtcgt ccgacatgtg
     2941 gtcgctgcaa tagaccaggc cgttgccgac gcggtgctgc agcgggattt tccagcgcca
     3001 cccggccttg tgcgcgatgg cccgggtgta gggcacggcg ggctctgtcg aggtggtttg
     3061 cacggccagg gcgctgttgg tcggcaacca atggctccag tcctcccagc cggccttcag
     3121 ggtctgttcg atcagcaggc cgcgaaagcc ggtgcagtcg atgaagagat caccctcgac
     3181 gatctcgccc gattccatga ccagggcctc gatgaaaccg tcttcagcgc gctgctgaac
     3241 ccgtgcgatc ttgccctcaa gccgccgtac gcccttgcct tcgctcatct tgcgaaggaa
     3301 gcggccatag agcgtggcgt ccagatgata ggcgtagttg gtcgtggcgt tctcgtcggt
     3361 gaagaacctg tcggcctcgg ccgccttcag ctcgaagcaa tagtcgccca gctcgccgcc
     3421 aaaaccctgg gccctggcct ggagccagaa atgatgaaag ccgcccatcc aggtggattt
     3481 gccgacctgg ccgaatgagt ggatataccg gtcgccgatc tggccccagt tctcgaacga
     3541 gatgcccagc ttgaaggtcg actgggtctc acgcatgaac gtggcttcgt cgatgccgag
     3601 cagggcgttg aaggtgcggg
//
LOCUS       DAOMEO010000196         3620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2938_length_3620_cov_2.777279, whole genome shotgun sequence.
ACCESSION   DAOMEO010000196 DAOMEO010000000
VERSION     DAOMEO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2938_length_3620_cov_2.777279"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1236)
                     /locus_tag="PLH31_05745"
     CDS             complement(253..1236)
                     /locus_tag="PLH31_05745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HQR88764.1"
                     /translation="MRALVVEALAPDYAGCAVREVAVPKPGPGQVLVRVRAASVNFPD
                     LLMTRGEYQFKPPTPFTPGLDLSGEVAALGEGVTSFAIGDAVVGGARLGAFAEYAVVS
                     TDALKPKPERLSFAQAAAYGAAYLTAYVALVRRARIEPGDWVLVHGAAGGVGLAAVDL
                     AKALGARVIAASASDDKLAAIQGLYAPDAIVNVTGGFRDQVKAITGGRGADIIYDPVG
                     GDVFDESVRCIAFDGRLLVIGFTSGRIPTVSVNMPLIKGFSVMGVRAGEYGRQFPEKG
                     RENAQAVWKMADEGVITPHVHAELPLERWREAFELLAERKVVGKAIIRPDP"
     gene            complement(1393..2397)
                     /locus_tag="PLH31_05750"
     CDS             complement(1393..2397)
                     /locus_tag="PLH31_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88765.1"
                     /translation="MADVAVQKFILKTILSLPSPILRAMSGGGVVYRGGRTLDPRFQF
                     FAHAAKKMPSITTLPPGVAREASAKGLVAVSGPPEPGVRTENLSIDGPGGSLPLRAYR
                     PADQDSGAPLIVFAHFGGGVIGDLETCHAFCGILARLARTAVLSVDYRLAPDHRFPAG
                     LDDVLAAYRWGRDNAARFGALPGAAAIGGDSMGGNFAAIVAQEMKRTGEPQPALQLLI
                     YPAVDVASETTSMSTYADAYPLSRPIMDWFMGHYMGPDANPADPRLSPNKSDDLTGLA
                     PAVVITAGLQADGVPVTYRCYDSLAHGFTAFTGAVPAADEACREMARLVRAAFDAAKA
                     "
     gene            complement(2416..3054)
                     /locus_tag="PLH31_05755"
     CDS             complement(2416..3054)
                     /locus_tag="PLH31_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR88766.1"
                     /translation="MSNIAYFPKAPVVDDRPGKRERTKVANRQAILDAAREVFGDLGY
                     DAATVRDIIRRTGLASGTFYNYYKSKEEVFDALADDGARRFRPLLRVEVDKAVDFQSF
                     LRGAITAYFQFVATEQENWRLNRPAGEQIPHARATPEIVAVFNEVREGFAKVLELEHA
                     PAVDLDYLTAACIAVAREVSDKMLERRPVDTAGAADFAVKLILGGLPALPKL"
     gene            complement(3117..3263)
                     /locus_tag="PLH31_05760"
     CDS             complement(3117..3263)
                     /locus_tag="PLH31_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88767.1"
                     /translation="MSRSPSVSIDAKAALRIIVIGDKSAHDGDAWGRNSMADPSACGE
                     SLAP"
     gene            complement(3260..3511)
                     /locus_tag="PLH31_05765"
     CDS             complement(3260..3511)
                     /locus_tag="PLH31_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HQR88768.1"
                     /translation="MPRIDLDKDTRAKLTDGLSRYLKDELDLEVKGFDAQFLLDFITE
                     RLGPYYYNQGLHDAQALFQKKIESITDAVYELEQPTKGF"
BASE COUNT          589 a         1228 c         1255 g          548 t
ORIGIN      
        1 ggcgggctcg cacgagcttc gacggctgga atgtccgctt cacgaggtgg ctccgataat
       61 aatgggacgc gagggccgca aggcctccgc gagtgagggg cggtggataa gggaaggggc
      121 ggcggctgtc aacctgagtc ggccgctgaa actgtcatcc cggccaagcg aagcgccgag
      181 ccgggaccgc ggcgagcgcc ggcgtttccg gcggtcccgg ctcttcgcta cgctacggcc
      241 gggatgacac cgttatgggt ccggccgaat gatggccttg cccaccacct tgcgctccgc
      301 caacaactca aacgcctcgc gccaacgctc cagcggaagc tctgcgtgga catgcggcgt
      361 gatcacgccc tcatcggcca tcttccatac cgcctgcgcg ttctcgcggc ccttctccgg
      421 gaactggcgg ccatattcgc cggcccgcac gcccatcacc gagaagccct tgatcagcgg
      481 catattgacc gagaccgtcg gaatccgccc cgaggtgaag ccgatcacca gcagccggcc
      541 gtcaaaagcg atgcagcgca cgctctcgtc gaacacgtcg ccgccgaccg ggtcatagat
      601 gatgtcggcg ccgcgcccgc cggtgatggc cttgacctgg tcgcggaacc cgccggtgac
      661 gttgacgatg gcgtccggcg catacagccc ctggatcgcc gccagcttgt cgtcggaggc
      721 cgaggccgcg atcacccgcg cgcccagcgc cttggccaga tcgacggccg ccagccccac
      781 cccgccggcc gcgccatgca ccagaaccca gtcgccgggc tcgatccgcg cccgacgcac
      841 cagggcgaca taggcggtga gataggccgc gccataggcg gcggcctgcg cgaaggacaa
      901 tcgctcgggc ttgggcttca gggcgtcggt cgacaccacc gcgtactcgg cgaaggcccc
      961 cagccgcgcg ccgccgacca ccgcgtcgcc gatcgcaaag ctggtgacgc cctcccccag
     1021 cgccgcgacc tcgccggaga ggtccaggcc cggcgtgaag ggcgtgggcg gcttgaactg
     1081 gtattcgccg cgggtcatca gcaggtcggg gaaattgacg ctggcggcgc ggacgcggac
     1141 cagcacctgg ccggggccgg gcttcgggac ggcgacctca cgcaccgcgc agccggcata
     1201 gtcgggggcc agggcctcga cgacgagggc gcgcatcatg cccccttccc cctttgatgg
     1261 gggaagggtt ggggatgggg gtgacagcgg ctcaggatat ggctcgaccg cgcgaaatcg
     1321 gccggcgggg tcacccccat ccccgccctt cccctatcaa agggggaagg gagaagaaga
     1381 gcgcccatct cgctaagcct tcgcggcgtc gaatgccgcc cgcaccagcc gcgccatctc
     1441 gcgacacgcc tcgtcggccg ccggcacggc cccggtgaac gccgtgaagc cgtgggccag
     1501 gctgtcgtag cagcggtagg tcaccggcac gccgtcggcc tgcaggccgg cggtgatcac
     1561 cacggcgggc gccaggcctg tcaggtcgtc gctcttgttg ggcgacaggc gcggatcggc
     1621 ggggttggcg tcagggccca tatagtgacc catgaaccag tccatgatcg gccggctcag
     1681 cggataggcg tcagcatagg tgctcatgga cgtggtctcg ctggccacgt cgacggcggg
     1741 atagatcagc aactgcaagg cgggctgagg ctcgccggtg cgcttcatct cctgggcgac
     1801 gatggccgcg aaattgccgc ccatggagtc gccgccaatg gccgcagcgc ccggcaaggc
     1861 cccaaagcgg gccgcgttgt cgcggcccca gcgataggcg gccagcacat cgtccagacc
     1921 ggccgggaaa cggtgatcgg gcgccaggcg ataatccacc gacagcactg cggtgcgggc
     1981 gaggcgggcc aggatgccgc aaaaggcgtg gcaggtctcc aggtcgccga tcaccccgcc
     2041 gccgaaatgg gcgaagacga tcaggggcgc gccgctgtcc tggtcggccg ggcgataggc
     2101 gcggagaggc aaggacccac ccggcccgtc aatcgacagg ttctcggtgc gtacgccggg
     2161 ttccggcggg ccggacaccg cgaccaggcc cttggcgctg gcctcgcgcg ccacgccggg
     2221 cggcagggtg gtgatcgacg gcatcttctt ggcggcatgg gcgaagaact ggaatcgcgg
     2281 gtccagggtg cggccgccgc gatagactac ccctcccccc gacatggccc gcaggatcgg
     2341 gctgggcagg gacaggatgg tcttcagaat gaacttctgg acagcgacgt cagccatgcg
     2401 ggctccggat aaggattaca gtttgggcaa ggcgggcagg ccgcccagga tcagtttgac
     2461 ggcgaagtcg gccgcgccgg cggtgtcgac ggggcggcgc tccagcatct tgtcgctgac
     2521 ctcgcgggcc acggcgatgc aggcggccgt cagatagtcc aggtcgaccg cgggcgcgtg
     2581 ctccagctcc agcactttcg caaagccttc gcgcacctcg ttgaacaccg ccacgatctc
     2641 gggcgtggcg cgggcgtgag ggatctgctc gccggctggc cggttcagac gccagttctc
     2701 ctgctcggtg gccacgaact ggaaataggc ggtgatggcg ccgcgcagga acgactggaa
     2761 gtcgacggcc ttgtcgacct cgacccgcag caggggccgg aagcgccgcg cgccgtcgtc
     2821 ggccagggcg tcgaacactt cctctttgga cttgtagtag ttgtagaacg tgcccgaggc
     2881 gaggccggtg cggcggatga tgtcgcgcac cgtggcggcg tcatagccca ggtcgccgaa
     2941 cacttcccgg gccgcatcaa ggatggcctg gcggttggcg accttggtcc gctcgcgctt
     3001 gccgggccgg tcgtccacaa ccggcgcctt ggggaaatag gcgatattcg acatcgaacg
     3061 cgactcatgg acgaggcgat cgtgacgccc cgtcatgatt tgagcctcat tccgcctcac
     3121 ggggcaagac tttcgccgca ggcggacgga tcggccatgc tgttccttcc ccaggcgtcg
     3181 ccgtcgtgcg cggacttatc gccgatcacg atgatgcgca gcgcggcctt agcgtcaatg
     3241 ctgacgctgg gggaacggct caaaacccct tcgtcggctg ctccagctcg taaaccgcat
     3301 cggtgatgct ctcgatcttc ttctggaaca gcgcctgggc gtcgtggagg ccctgattgt
     3361 agtagtatgg ccccagccgc tcggtgatga agtcgagcag gaactgggcg tcgaagcctt
     3421 tgacctccag gtccagctcg tccttcagat agcgcgacag gccgtcggtc agcttggcgc
     3481 gggtgtcctt gtcgagatcg atcctgggca tgggcgggct ttcgattcct gtcagcgcac
     3541 cctagccact cgtcccctcc aggcctacgg ctctcagacg aggacgaccc tgtccgcccg
     3601 cgccgccacc ggcagccggt
//
LOCUS       DAOMEO010000197         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2941_length_3615_cov_2.668820, whole genome shotgun sequence.
ACCESSION   DAOMEO010000197 DAOMEO010000000
VERSION     DAOMEO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2941_length_3615_cov_2.668820"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PLH31_05770"
     CDS             <1..149
                     /locus_tag="PLH31_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR88769.1"
                     /translation="GMGASALLLGEGMPAWKPIAGALVLTGLAVNMLWPKVRGRFGGA
                     RIGA"
     gene            199..330
                     /locus_tag="PLH31_05775"
                     /pseudo
     CDS             199..330
                     /locus_tag="PLH31_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516778.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            546..1685
                     /locus_tag="PLH31_05780"
     CDS             546..1685
                     /locus_tag="PLH31_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HQR88770.1"
                     /translation="MATPFKVEWSQEAIDKVLAQVRAYEFPPAPEEGGWNHGCDADTL
                     KRLTAYWTDGFDVAKAVDTLNRYPQFTARVEDLDIHFVHVIGEAGGKRPLLLTHGWPG
                     SHFEFWDAIEPLAFPSRHGGDPADAFDLVIPSLPGFGFSGKPAKPIGQRTTARLFNSL
                     MTQELGYDRYLAQGGDWGGLVTSWLGLDHSAHVKAIHLNMIGFRPSGPPQGEAEIAWI
                     QRTGFAMDVMGAYFRLQASKPQSLAWLGAGNPVGQAAWILERFHDWADLSAKPFEAAF
                     SDDQLLTNIMLYVMTGSFTTGAWYYRGLMEEGGIVLPAGTRCETPTAFANFPGEALYQ
                     APPRSWCDRAYNIVRWTDMPRGGHFGAMEEPVLFVEDVRAWARQA"
     gene            1708..2115
                     /locus_tag="PLH31_05785"
     CDS             1708..2115
                     /locus_tag="PLH31_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR88771.1"
                     /translation="MTLKTGGCLCGAIRYEIDADFMGVAVCHCRDCQYASGGGPNFIA
                     MAPRDSVRFVEGAPKRYDRPGGSGATVGRVFCPDCGTPLLSDIGAPFLAVKLGGLDDA
                     ADLKPGVRIWTSSAPPWHVIDPATPGFPENPPF"
     gene            2115..3140
                     /locus_tag="PLH31_05790"
     CDS             2115..3140
                     /locus_tag="PLH31_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HQR88772.1"
                     /translation="MTVARYDFASDNVAGAMPEVMEALVVANAGTASGYGTDHVSAAA
                     AERIRALLDADAQVRFTASGTAANAFALTLLAQPHEAVLAHEHAHICTDETGAPGFFG
                     QGVGLIGLPGASGRIELAGLEAALARPEVSYNQPAAALSLTTATEYGTVYDEAHLRSL
                     IAPVKAKGYGVHLDGARLANAVAAGFDLKSIARMGVDILVMGGTKAGSTPTEAVVFLN
                     PDHARRLDARLKHAGQLVSKGRFLAAPWLGLLGEGADGGPWAARAAHANAMAHKLAEL
                     MPFAVRHPVEANGVFVDMDDAALDRLRGEGWFVYRFLDGTARFMCSWATTPEMVEDLG
                     AALKRIA"
     gene            complement(3362..3615)
                     /locus_tag="PLH31_05795"
                     /pseudo
     CDS             complement(3362..3615)
                     /locus_tag="PLH31_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          561 a         1254 c         1190 g          610 t
ORIGIN      
        1 tcggcatggg cgcctcggcc ctgctgctgg gcgaagggat gcccgcctgg aagccgatcg
       61 cgggggccct ggtgctgacc ggtctggcgg tgaacatgct gtggccgaag gttcggggaa
      121 ggttcggggg ggcgcgtatc ggcgcatagg tacagtctgg aattgatttt ttagccgccc
      181 ggtccaagct agggcatggt tcctcgcgag aagcggttga aggagtagcc tcgccagtgg
      241 aagatcaatc tgatcgagcg cggcaatccg cactgggatg acctctacga tcaagtgatg
      301 aactggacgc ccgttccgcg ccaagtctag cgctttctct tcaacaaatc ccgtcatccc
      361 gcgctttatg cgcgggaccc atttgtccgc cgcacctgca ttgcggaccc gagctctcac
      421 gttgaagctg aaccatgggt cccgcgcatg aagcgcggga tgacggcaaa aggggagagc
      481 ggagttttag ttgacctccg ccgcgtcacc cgcctgaccc tccccgcaaa acagggagag
      541 acgccatggc cacgccgttc aaggtcgagt ggagccagga agccatcgac aaggtgctgg
      601 cccaggtccg cgcctatgag tttcctcccg cgcccgaaga gggcggctgg aaccatggtt
      661 gcgacgccga caccctcaag cgcttgaccg cctactggac cgacggcttc gacgtcgcca
      721 aggccgtcga caccctcaac cgctatccgc agttcaccgc ccgggtcgag gacctggata
      781 ttcacttcgt ccacgtgatc ggcgaggccg gcggcaagcg tccgctgctg ctgacccacg
      841 gctggccggg cagtcacttc gagttctggg acgccatcga gcccctggcc ttcccctcgc
      901 gccatggcgg cgatccggcc gacgcctttg atctggtgat cccgtccctg cccggctttg
      961 gcttctccgg aaaaccggcc aaaccgatcg gccagcggac cacggcgcgg ctgttcaaca
     1021 gcctgatgac ccaggaactg ggctacgacc gctatctggc ccagggcggc gactggggcg
     1081 gcctcgtcac ctcgtggctg ggactggacc attcggcgca cgtcaaggcc atccacctga
     1141 acatgatcgg ctttcggccc agcggcccgc cgcagggtga ggccgagatc gcctggatcc
     1201 agcgcacggg tttcgccatg gacgtcatgg gggcctattt ccggctgcag gcctccaagc
     1261 cccagtccct ggcttggctg ggggcgggca atcccgtggg tcaggccgcc tggatcctcg
     1321 agcgtttcca cgactgggcc gacctgtcgg ccaagccgtt cgaggcggcg ttcagcgacg
     1381 accagctgct gaccaacatc atgctctatg tgatgaccgg cagcttcacc accggcgcct
     1441 ggtactatcg cggcctgatg gaggaaggcg ggatcgtgct gccggccggg acgcgctgcg
     1501 aaacccccac cgccttcgcc aacttccccg gcgaggccct gtatcaggcg ccgccgcgca
     1561 gttggtgcga ccgggcctac aacatcgtcc gctggaccga catgccgcgc ggcggccatt
     1621 tcggcgccat ggaagaacct gtcctgttcg tcgaagatgt gcgcgcctgg gcccgccagg
     1681 cctgatcctg aaaaccggag acctgtgatg actctcaaga ccggcggctg cctgtgcggc
     1741 gccattcgct acgagatcga cgccgatttc atgggcgtcg ccgtttgcca ctgcagggac
     1801 tgccagtacg cctcgggcgg cggccccaac ttcatcgcca tggcgccgcg cgacagcgtg
     1861 cggttcgtgg aaggcgcgcc caaacgttat gaccggccgg gcggcagcgg cgccacggtc
     1921 ggtcgcgtct tctgccccga ctgcggcacg cccctgctgt cagacatcgg cgcgccgttc
     1981 ctggccgtga aactgggcgg gctggacgac gccgccgatc tcaagcctgg cgtacgcatc
     2041 tggaccagct cggcgccgcc gtggcatgtg atcgaccccg ccacgcccgg ctttccagag
     2101 aatccgccgt tctgatgaca gtagcccgtt acgacttcgc ctccgacaat gtcgccggcg
     2161 ccatgcccga ggtgatggag gcgctggtcg tggccaatgc gggcacggcc tcgggctatg
     2221 gaaccgacca tgtcagcgcc gccgccgcag agcgcatccg cgctctgctc gacgcggacg
     2281 cccaggtgcg gttcaccgcc tcgggcacgg cggccaacgc ctttgccctg accctgctgg
     2341 cccagccgca cgaggctgtg ctggcccacg agcacgccca catctgcacc gacgagaccg
     2401 gcgcgccggg cttcttcggc cagggcgtgg gcctgatcgg cctgccgggc gcttcggggc
     2461 ggatcgagct ggcgggcctg gaagcagccc tggcgcggcc cgaggtctcc tacaaccagc
     2521 ccgcagcggc cctgtccctg accaccgcca ccgaatacgg tacggtctat gacgaggctc
     2581 atctgcggtc gctgatcgcg ccggtgaagg ccaagggcta tggcgtccac ctggacggcg
     2641 cgcggctggc caacgcggtg gcggcgggct ttgaccttaa gagcatcgcc cgcatgggcg
     2701 tcgatatcct ggtcatgggc gggaccaagg ccggctcgac cccgaccgag gcggtggtgt
     2761 tcctcaatcc cgaccacgcc cggcgactgg acgcccggct caagcacgcc ggccagttgg
     2821 tctccaaggg ccgcttcctg gccgccccgt ggctgggcct gctgggcgaa ggcgccgacg
     2881 gcggcccctg ggccgcgcgc gcggcccacg ccaacgccat ggcccacaaa ctggcggaac
     2941 tgatgccctt cgcggtccgc catccggtcg aggccaatgg cgtctttgtc gacatggacg
     3001 atgccgccct ggaccgcctg cgcggcgagg gctggttcgt ctatcgcttc ctggacggaa
     3061 cggcccgctt catgtgctcc tgggccacga cgcccgagat ggtcgaggac ctgggcgcgg
     3121 cgctcaagcg gatcgcttaa tcttagatgc tccccctttg ggggagctgt cgcggagcga
     3181 ctgagggggc cttctcggcc tccgccgagc tacccccctc cggccctctg ggccacctcc
     3241 cccaaagggg gaggagctag gccgtcgctt ttttcaagcc cttggcccgg cgcgccttcg
     3301 gagccagctt ttccttctgg gcaaacgtca acacggcgtc gctcgctgga acgcgcagat
     3361 cccgcgcctc cacacccagc aatcgcgcga tgtctgacgc cggcgccggg cggctgatga
     3421 aataaccctg gatctcggcg caaccttggt cgcgcagggc gttgagctgc tcgaccgact
     3481 ccacgccctc ggccgtggtg acgatgccca ggctggaccc caggtcgagc acggccttga
     3541 tgatggccat ggcgtcgctg tcttcgagga tgtcgcggac gaaggtctgg tcgatcttga
     3601 tcttgtcgaa cggga
//
LOCUS       DAOMEO010000198         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2952_length_3602_cov_2.246123, whole genome shotgun sequence.
ACCESSION   DAOMEO010000198 DAOMEO010000000
VERSION     DAOMEO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2952_length_3602_cov_2.246123"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLH31_05800"
     CDS             complement(<1..996)
                     /locus_tag="PLH31_05800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HQR88773.1"
                     /translation="MSTVIKPEDLLCPRPNGLYCPPGDFYIDPVRPVDRAVITHGHAD
                     HARAGHGVVAATPETLAIMAVRYGEDFAGRREVVPYGGSFTHNGVEVTLVPAGHVLGS
                     AQAVVRWKGLTMVVSGDYKRRRDPTCARFEPIPCDVFITEATFGLPVFRHPDDRGEIA
                     SLLASVAQFPERTHLVGAYALGKAQRVIRLLREGGWDQPIYVHGALERLNALYEDHGV
                     DLGPLLPATTGQAKKDFASQIVIAPPSAIADRWSRRFADPVDCFASGWMRVRARARQR
                     GVELPLILSDHADWDELTATLSELSPGEVWITHGREEALLRWCELQGLPARALRLV"
     gene            1027..1530
                     /locus_tag="PLH31_05805"
     CDS             1027..1530
                     /locus_tag="PLH31_05805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQR88774.1"
                     /translation="MSDDNEQSRLIATAMNEGSPQARALGFTTLKIGDAMAILKVPYR
                     PEIVGDPETGVIAGGVMTTLLDHASGQAVHAALETWTSIATLDLRIDYMRPAQPGRDV
                     LARAHCYKLTRSVAFVRAVAYEDDPEDPVATAQATFMLDSSAGKKAGANLRPSKAPGA
                     KTGGDAA"
     gene            1527..1946
                     /locus_tag="PLH31_05810"
     CDS             1527..1946
                     /locus_tag="PLH31_05810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQR88775.1"
                     /translation="MSDADNRLVAVLGSIPYARFLGVTAELAGDEMTAILPFADHIVG
                     NPMLPAIHGGVLGAFMEMTALAQLSVAAPLKRQPRTVDVSIEYLRSGRPLTTYARATI
                     KKLGRRVANVHVEAWQETRAAPIATLRGIFLVAPSEE"
     gene            1949..2143
                     /locus_tag="PLH31_05815"
     CDS             1949..2143
                     /locus_tag="PLH31_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HQR88776.1"
                     /translation="MSRASRGGQSTGRGGGKVVRKGDLPSKPCLVCGRPFTWRKKWEA
                     VWEEVKYCSDRCRSHKITPM"
     gene            2277..2372
                     /locus_tag="PLH31_05820"
     CDS             2277..2372
                     /locus_tag="PLH31_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88777.1"
                     /translation="MTNTNAFAGFATLVLAVLPLVVIAGSLATSF"
     gene            complement(2399..3322)
                     /locus_tag="PLH31_05825"
     CDS             complement(2399..3322)
                     /locus_tag="PLH31_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HQR88778.1"
                     /translation="MMMSPPIVHHPAFRAEMPEGHRFPMDKFSRLAALLEREGLAGPA
                     GFHRPEPVDLETLRLVHTEDYVRGVVEQSLPAEIVRRIGMPNSESVSTRARAATGGTL
                     LTARLALEHGLACNTAGGSHHASAESGAGFCVFNDVAVAARRLQAEGLVGKVLVVDLD
                     VHQGDGTARIFEDDPSVFTFSMHAEKNFPARKAVSDLDIDLPDGTGDEVYLATLAEIL
                     PALLARERPDLVFFNAGVDPHVDDKLGRLSLGDDGLARREAFVLGCCLERGIPVAGVI
                     GGGYDADIDRLAHRHAILHRAAREAFSAWGR"
     gene            3396..>3602
                     /locus_tag="PLH31_05830"
     CDS             3396..>3602
                     /locus_tag="PLH31_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR88779.1"
                     /translation="MQTPENLAEILDLEAIEVNLFRGSSPNDGFPRIFGGLVIAQALL
                     AAYKTVPERVCHSLHAYFIRPGDVN"
BASE COUNT          647 a         1266 c         1154 g          535 t
ORIGIN      
        1 caccagccgc agcgcccggg ccggcagacc ctgcagttcg caccagcgca gcagggcctc
       61 ttcgcgcccg tgcgtgatcc acacctcgcc ggggctcagc tccgacagcg tcgccgtcag
      121 ctcgtcccag tcggcgtggt ccgacaggat gagcggcagc tcgacgccgc gctgcctggc
      181 ccgggcccgc acccgcatcc agccggacgc gaaacagtca acgggatcag cgaaacggcg
      241 cgaccagcga tcggcgatgg ccgagggcgg ggcgatgacg atctggctgg cgaagtcctt
      301 tttcgcctgg ccggtggtgg ccggcagcag cggccccaga tcgacgccgt ggtcctcgta
      361 gagcgcgttc agccgctcca gcgcgccatg cacatagatc ggctgatccc agcccccctc
      421 gcgcagcagc cgtatcaccc gctgggcctt gcccagggca taggcgccga ccaggtgggt
      481 gcgctcggga aactgggcga cgctggccag gaggctggcg atctcgcccc ggtcgtcggg
      541 gtggcggaac accggcaggc cgaaggtggc ctcggtgatg aagacgtcgc acggtatggg
      601 ctcaaaccgc gcgcaggtcg gatcgcggcg gcgcttgtag tcgccggaga ccaccatggt
      661 caggcccttc cagcgcacca ccgcctgggc cgagcccagc acgtggcccg ccgggaccaa
      721 agtcacctcg acgccattgt gggtgaacga cccgccgtag ggcacgacct cgcgacgacc
      781 ggcgaaatcc tcgccatagc ggacggccat gatcgccagg gtctcgggcg tggccgccac
      841 cacgccgtga ccggcgcggg catgatcggc atggccatgg gtgatcaccg cccggtccac
      901 aggacggacc gggtcgatat agaagtcccc cggcgggcag tagaggccgt tcgggcgggg
      961 acagagaagg tcttcgggct tgatcacggt agatatgaca acgccgccac tgctaatcct
     1021 atccccatga gcgatgacaa cgaacaatcc cgcctgatcg ccacggcgat gaacgagggc
     1081 agcccccagg cgcgggccct gggtttcacc accctgaaga tcggcgacgc gatggccatt
     1141 ctcaaggtcc cataccgccc cgagatcgtc ggcgaccccg agaccggcgt gatcgccggg
     1201 ggcgtgatga ccaccctgct ggaccacgcc agcggccagg ccgtgcatgc ggcgctggaa
     1261 acctggacat ccatcgccac gctggacctg cggatcgact atatgcggcc ggcccagcca
     1321 ggccgcgacg tgctggcccg ggcccattgc tacaagctga cccgctcggt cgcctttgtc
     1381 cgggccgtgg cctatgaaga cgatcccgag gacccggtgg ccacggccca ggccaccttc
     1441 atgctcgaca gcagcgccgg caagaaggcc ggagccaatc tgaggccctc caaggcccca
     1501 ggcgccaaga cagggggaga cgccgcatga gcgacgccga caaccgcctg gtcgccgttc
     1561 tcggctcgat cccctacgcc cggtttctgg gcgtcaccgc cgaactggcc ggcgacgaga
     1621 tgaccgccat cctgcccttc gccgatcaca tcgtcggcaa tccgatgctg ccggcgatcc
     1681 atggcggggt gctgggcgcc ttcatggaga tgacggccct ggcccagctg tcggtggccg
     1741 cgccgctgaa gcgccagccg cgcacggtcg atgtctcgat tgaatatctg cgctcgggcc
     1801 gtccgctgac cacctatgcc cgcgccacga tcaagaagct gggacgccgg gtcgccaacg
     1861 tccacgtcga ggcctggcag gaaacccgcg ctgcgcccat cgccaccctg cgcggcatct
     1921 tcctggttgc gccctcggag gaatgaccat gagccgggcc tcgcggggcg gccaaagcac
     1981 gggcaggggc ggcggcaagg tcgtgcgcaa gggcgatctg ccgtcgaagc cctgcctggt
     2041 ctgtggtcgg ccgttcacct ggcgcaagaa gtgggaagcg gtctgggagg aagtgaaata
     2101 ttgctcagat cgctgcagat cacataaaat tacaccaatg taatgctaat tgatggctgg
     2161 aagtttctta aaaagccttc atgacgcaaa ggtaacgggt attcaccaaa cggcgagcct
     2221 atatccctgt tcaccggacg cgaacaaccg cgccggacca caaagggatc aagaccatga
     2281 ccaacaccaa cgctttcgcc ggcttcgcca ccctggttct cgcggttctg ccgcttgtcg
     2341 tgatcgccgg ttcgctcgcc acgagcttct gaatgaccgc cgcgtagctc caccgggtct
     2401 atcgccccca ggctgaaaag gcctcgcggg ccgcccggtg gagaatggcg tggcgatgcg
     2461 ccaatcgatc gatgtcggcg tcgtatccgc cgccgatcac ccccgccacg gggatacccc
     2521 gctccagaca acagcccagg acgaacgcct cgcgccgtgc gagcccgtca tcgcccaggg
     2581 acagacgccc cagcttatcg tcaacgtgcg gatcgacccc ggcgttgaag aacaccagat
     2641 ccggcctttc ccgcgccagc agggccggca ggatttccgc cagcgtcgcc agatagacct
     2701 catcccccgt accatcgggc aggtcgatat ccagatcgct gacagccttg cgggccggaa
     2761 agttcttctc ggcatgcatc gagaaggtga agacgctggg atcgtcctcg aagatccggg
     2821 ccgttccatc gccctgatgg acatccagat cgaccaccag aaccttgccg acgaggcctt
     2881 cagcctgcag ccgccgcgcg gccaccgcca cgtcgttgaa cacgcagaaa cccgccccgc
     2941 tctcggccga cgcgtggtgg ctgccgccgg ccgtgttgca ggccaggccg tgctccagag
     3001 ccagtcgcgc ggtcagcagc gtgccgcccg tcgccgcccg cgcccgcgtc gataccgact
     3061 cgctgttggg catgccgatc cggcggacga tctcggcggg aagcgactgc tcgaccacgc
     3121 cgcgcacata gtcttcggta tggaccagcc gcagggtttc gaggtcgaca ggctcgggac
     3181 gatgaaatcc agccggtccg gccaggcctt cacgctcaag cagggcggcc aggcgcgaga
     3241 acttgtccat cggaaaccgg tgcccctccg gcatctcggc gcggaaggca gggtggtgca
     3301 cgatgggcgg cgacatcatc aaacgatggc gatcgggacg acatgacgca aggtcccgtc
     3361 ttgccccggc gacgcgcggg cattaaccca gcgccatgca gacgcccgaa aacctcgccg
     3421 aaatcctcga cctcgaagcc atcgaggtga acctgttccg gggctccagc cccaatgacg
     3481 ggtttccacg catctttggc gggctggtca tcgcccaggc cctgctggcc gcctacaaga
     3541 ccgtgcccga gcgggtctgc cactcgctgc atgcctattt catccggccc ggcgatgtga
     3601 ac
//
LOCUS       DAOMEO010000199         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2956_length_3600_cov_2.442595, whole genome shotgun sequence.
ACCESSION   DAOMEO010000199 DAOMEO010000000
VERSION     DAOMEO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2956_length_3600_cov_2.442595"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLH31_05835"
     CDS             complement(<1..885)
                     /locus_tag="PLH31_05835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR88780.1"
                     /translation="MAREERGVAPRVGVTATAFAADEIPPPLSQAAEGAHVTFGRWQR
                     TTVAAMLLGILVLGVLSPDHLFWGLLGVFYSVFVAGAAIRLAAILTPHRKWACPPLVD
                     QDLPHYTLIVPLYREAEVASELVANLARLDYHRDRLQALIVLEADDLATRSAFHALDL
                     PAFIQVLVAPPGEPRTKPRACNIALELAKGTLVVIYDAEDAPDPLQLREAAARFASEP
                     ADLACLQAPLRIETPRHLAFIAEQFRLEYAALFQVLLPAYARWGLPFPLGGTSNHFKI
                     EALRAVGGWDAYNVTEDAD"
     gene            1057..1692
                     /locus_tag="PLH31_05840"
     CDS             1057..1692
                     /locus_tag="PLH31_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR88781.1"
                     /translation="MTLVLKKPHKSARKPKGDGHLRRGEILAAAERIFIAEGYAGATI
                     RKIADAVGVSSTALYMHFRDKDQILLEISDGAIERLLNLNIDISARPVDPVSQVRLML
                     EAYMRFALDNPNTYELVFCGSGASISVEKQAGTLELGDRCFENFSEPFREIAAAGRLK
                     SGTAQSAAEVAWAGCHGLVSLLITKPNRTWSSSDDLMSLMLDGLLDGLVKD"
     gene            1697..2509
                     /locus_tag="PLH31_05845"
     CDS             1697..2509
                     /locus_tag="PLH31_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQR88782.1"
                     /translation="MMIVRAIGALAAAMTLAHPAVAAGPRVMSLDSCADQYVLALAPR
                     ETIVGLSHRAGAPDSYMRKASLGLPRRRATTESLVAAGPQVVVRHWGGDARLVKALDR
                     RGVRTVRIEDAADFEAVRVNVRRVAEALDQKAAGEAMIARMDGQLRAGAGAWKGETAL
                     YLTPGGYTAGSGTLIDAMLRASGLSNASNGPFFSPVPLESVVMAPPRALVLGFFDLAR
                     AGADRWGVGHHAALRRVTRDRTIAALPGAVVGCPAWFAADGVAQLAQARALR"
     assembly_gap    2520..2529
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2530..3505
                     /locus_tag="PLH31_05850"
     CDS             <2530..3505
                     /locus_tag="PLH31_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQR88783.1"
                     /translation="RLCLGMLCLLVLAAVMAVLLGETSFVIGQYMEAVAHPGSPPGEV
                     LWTIRAPRVAMAALVGAALGLAGATMQGLLRNPLADPGVLGVSAVSGLGAAVAIASGL
                     AAVPGAIELAALAGALAAGGAVVTIVARLREPEALILLGVAVSAFGGALTALVFNLAP
                     SPVAIAEVLAWLMGSVENRDWTDSFKALVPMAIGAALCLKAGRGLRMLTLGEEAAAMS
                     GLPMSRLRVYAVAGAALLTGAAVAGAGVIGFVGLAAPHMVRALVKDDPRQVLWPSALA
                     GALLLVLADLAARLSPTEQELKLGVVTALFGAPVFALLAWRASRSWRA"
BASE COUNT          551 a         1110 c         1295 g          634 t
ORIGIN      
        1 gtcggcgtct tcggtgacgt tgtaggcgtc ccagccgccg acagcccgca aggcctcgat
       61 tttgaagtgg ttgctggttc cgcccagcgg gaagggcagg ccccagcgcg cataggcggg
      121 cagcagcacc tggaagaggg cggcgtactc cagccggaac tgctcggcga tgaaggccag
      181 gtgacgcggg gtctcgatgc gaagcggggc ttgcaggcag gccagatcgg ccggttcaga
      241 cgcaaaccgc gccgccgctt cgcgcagttg cagcggatca ggggcgtctt cagcgtcgta
      301 gatgacaaca agcgtcccct tggccagttc cagggcgatg ttgcaggccc gcggcttggt
      361 gcgcggctcg ccgggcgggg cgaccagaac ctggatgaag gcgggcaggt ccagggcgtg
      421 gaaggctgac cgtgtcgcca gatcgtccgc ctcaaggacg atcagcgcct gtagtcgatc
      481 gcggtggtag tccagtctgg caagattggc gaccagttcg ctggcgacct cggcttcacg
      541 ataaagcggc acgatcagcg tgtagtgcgg caggtcctga tcgaccagcg gggggcaggc
      601 ccactttcgg tgtggcgtca ggatcgccgc gagccggatc gcggcgccgg cgacgaagac
      661 gctgtagaac acgccaagca gcccccagaa caggtgatcg ggagacagaa caccaagaac
      721 gagaatgccc agcagcatgg cggccactgt tgttctttgc catcggccga aggtgacgtg
      781 ggcgccctcc gccgcctgcg acaggggtgg cggtatctcg tcggcggcaa acgccgtggc
      841 ggtgaccccc accctgggcg cgacgccccg ctcctcgcgc gccataatgc cccccggctg
      901 gttcgctcga tcttcgcggg attactagga tcaacacaac taataagtgt caaacataaa
      961 aacacgcgtt ttaggtttga tgttgtgtcg cttgcgacgg ctgggtgatt cccgtttagt
     1021 gtcggagatc ggggaaacgc aggggaaatg caggccgtga cgcttgtttt gaagaagccg
     1081 cacaagtccg ctcgcaagcc aaagggcgac gggcacttgc gtcgcggcga aatcctcgcg
     1141 gccgccgaaa gaattttcat tgccgagggt tatgcgggcg ccaccatccg caagatcgcc
     1201 gatgcggtcg gggtctcgtc gacggcgctt tacatgcact tccgcgacaa ggatcagatc
     1261 ctgctcgaga tcagcgacgg cgcgatcgag cggctgctga acctcaatat cgacatctcg
     1321 gcccgacccg tcgatccggt ctcgcaggtt cggctgatgc tggaggcgta catgcgcttc
     1381 gcgctggaca accccaacac ctacgaattg gtgttttgcg gctctggcgc gagcatctcg
     1441 gtggaaaagc aggcaggcac gctggagctt ggcgatcgct gcttcgagaa tttcagcgag
     1501 cccttccgcg agatcgccgc agccggacga ctgaaatcag gcacggcgca gagcgctgca
     1561 gaagtggcgt gggcagggtg tcacggtctg gtgtcgctgc tgatcaccaa gccaaaccga
     1621 acctggtcgt cgtccgacga cctgatgagc ctcatgctcg atggcctctt ggacggcctg
     1681 gtcaaagact gatccgatga tgatcgtccg cgcgattggc gccctcgcgg cggcgatgac
     1741 ccttgcccat ccggctgtcg ccgcagggcc acgcgtgatg tctctggatt cctgcgccga
     1801 tcagtatgtc ctggccctgg cgccgcgcga gacaattgtt ggcctgtctc atcgggccgg
     1861 agcgccggat tcctatatgc gcaaggcgtc gcttgggctc ccgcgacgcc gcgcgacgac
     1921 ggaaagcctg gtggccgctg gcccccaggt cgtggtccgc cattggggcg gagacgcccg
     1981 actggtgaag gccctggatc gacgcggcgt ccgcacggtc aggatcgaag acgccgccga
     2041 tttcgaggcg gtgcgggtca atgtccgtcg tgtggccgaa gcgctggacc agaaggcggc
     2101 gggcgaggcg atgatcgccc gaatggacgg ccaattgaga gcgggcgccg gcgcctggaa
     2161 aggtgaaacc gccctctatc tgacaccggg cggctatacg gcgggaagcg gcaccctgat
     2221 cgacgctatg ctcagggcct cgggcctgtc caacgccagc aacggtccct tcttcagtcc
     2281 cgtgccgctg gagagcgttg tgatggcgcc gccgcgcgcc ttggtgctgg gcttctttga
     2341 cctggcacgc gcgggcgccg accgctgggg cgttgggcac cacgcggcgc tgcgccgtgt
     2401 gacgcgggat cgcacgatcg ccgccttgcc cggcgcggtg gtaggctgtc cggcgtggtt
     2461 tgcagctgac ggggtcgcgc agctggccca ggcgcgggcc ctgcgatgag cccgctacgn
     2521 nnnnnnnnna cgtctgtgtc tggggatgct ttgtctgctc gtgcttgcgg cggtcatggc
     2581 cgtgctgctg ggcgaaacct catttgtcat cggccagtac atggaagcgg ttgcacaccc
     2641 aggctcaccg ccaggcgaag tgctgtggac catccgggcg ccgcgggtgg ccatggcggc
     2701 gctggtcggc gcggccctgg gtcttgctgg cgcaacgatg cagggcctgc tgcgcaatcc
     2761 tctggctgac ccgggcgtac tgggcgtctc ggcggtgtca ggtctgggcg ctgcagtggc
     2821 gatcgccagc ggactggcgg ccgtgcctgg agccatcgaa ctcgcggccc tggccggcgc
     2881 tctggcagcg ggcggggccg tggtgaccat tgtcgctcgc ctgcgcgagc ccgaggccct
     2941 gatcctgctc ggcgtcgccg tctcggcctt cggaggcgcg ctgacggctc tggtgttcaa
     3001 tctggcgcca tcgccggtgg ccatcgccga ggtcctggcg tggctgatgg gatcggtcga
     3061 gaatcgtgac tggacagaca gcttcaaggc cctggtcccg atggcgattg gcgcagcgct
     3121 ttgcctcaag gccggccgag ggctgcgcat gctgaccctg ggcgaagagg ctgccgcgat
     3181 gtcggggctg ccgatgtcgc gtctgcgggt ctatgccgta gccggcgcgg cgttgctgac
     3241 gggggccgca gtggctgggg ccggtgtcat cggtttcgtg gggctggctg cgccgcacat
     3301 ggtgcgggcg ctggtgaaag acgatccgag gcaggtcctg tggccttccg ccctggccgg
     3361 cgctctgctg ctggtgctgg ctgacttggc cgcgcgtctc tcgccgaccg agcaggagct
     3421 gaagctgggc gtggtgacgg cgctgtttgg cgccccagtc ttcgccttgc tggcctggcg
     3481 ggcgtcgcgg agctggcggg cgtgacggcg gcctggaccc taagcggcct gaaggctcag
     3541 caaggcgaca aacaggtgct ggacgccgcc agccttgtgg tcagccccgg cgaggtggtt
//
LOCUS       DAOMEO010000200         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2958_length_3598_cov_2.592436, whole genome shotgun sequence.
ACCESSION   DAOMEO010000200 DAOMEO010000000
VERSION     DAOMEO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2958_length_3598_cov_2.592436"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLH31_05855"
     CDS             <1..775
                     /locus_tag="PLH31_05855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR88784.1"
                     /translation="DEFPGLGRRIFKLNARKVHRAGNHVTRLLVVLEDVTEARLLERH
                     RDILAAELAHRIKNSLQIITSFVSFEIRRAAEPCVEGYRAMQARIGAVAELYDVISRS
                     AALGPVPVEPYLAGIAASLHSSLIGQAADIEILVEAEALLIDPDKAVSVGLIVNELAT
                     NAVKHAFPSGGGRIFLSFERRDEEVLLIVRDDGVGHDQARNSGVRSGLGSRYVEAFVK
                     QLGATIARASGAGGTTMTVKLPASILVEDVAPPPEAVAP"
     assembly_gap    982..991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <992..1436
                     /locus_tag="PLH31_05860"
     CDS             <992..1436
                     /locus_tag="PLH31_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88785.1"
                     /translation="DAVNWTGSDFAIFGVMLAVPLAILELTLRATGNLAYRAAVVIAL
                     GGAFLMTWANLAVGLVGDENNPLNLMFFGVLGVGLAGAVVAGFAAGGLARAMAAMAVA
                     QGLAGLAALIAGHVTIVLTGIFVLVWLASAGLFHKAARQQGAAPA"
     gene            1533..>2585
                     /locus_tag="PLH31_05865"
     CDS             1533..>2585
                     /locus_tag="PLH31_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQR88786.1"
                     /translation="MRVSSPSTLLLGVVFFCIAAALAAISLATAPAWLGLSVSASSAA
                     GPLVVRDLDPRGPAAVAGVRTGERLLAVIAPDGRQTPLTGDMLEEDDGATQAAADAGM
                     RRHDVAAQALKARPLRLKVQDPAGNSRTVTLDERPRPLSSLGAEFWANLSFGLIAALI
                     CGWVWALRPLELATQLFALTGLSMLAFTFGWAVHSALELAMDTALLGPVLWLNMAGAV
                     AYGGAMIAFFLCYPKRLAPAPVLLLMAPVAVALWAAMPLLPDNPSISSMGQGFTVVEM
                     ALIIVAIGVQTFASRRDAAAMAATRWLGLSVIIGAGAFIGGVAVPSAFGRPAIIDPRF
                     ALGFFVIIYVGMAFGLR"
     assembly_gap    2586..2595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2596..>3598
                     /locus_tag="PLH31_05870"
     CDS             <2596..>3598
                     /locus_tag="PLH31_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQR88787.1"
                     /translation="HKLFELDQWAFRILFYLGAGLALLALDAVLLTVLRYSPQASLGA
                     ALLVVAFAYLPFRDVLWRRTVARRTMKDHEMFAAVAQVALTHQAQAREAAWRDLLARI
                     YEPLEVTTASPPPPSATIVDEGVAMSLPAAAGAPALLLRFPARGRGLFGTAQVQLADQ
                     LVTLLTQIEQGRDAYARGVVEERLRIARDLHDDVGARLLSGLHRKELNQTHEAMRGAI
                     GDLRAIVSGLSGHELPLDEVLADLRHETAERLAAAGLSLDWPPPDLDEPSAPAPYRVY
                     KNLTSACREVISNAIRHAGASKVAVTVLHTKERLEMTISDDGVGLPREPGGRRSGLAN
                     "
BASE COUNT          464 a         1217 c         1288 g          609 t
ORIGIN      
        1 ggacgagttt ccggggcttg gccggcggat cttcaagctc aacgcccgca aggttcatcg
       61 ggcgggcaat catgtgaccc gcctgctggt ggttctggag gatgtcaccg aagcgcggct
      121 gctggagcgc catcgcgata tcctggccgc cgagctggcc caccgcatca agaacagcct
      181 gcagatcatc acctcgttcg tgtcgttcga gatccgccgc gcggccgagc cctgcgtcga
      241 gggctatcgc gccatgcagg cgcggatcgg cgcggtggcc gagctgtatg acgtgatctc
      301 ccgatcagca gcgctgggcc cggtgccggt ggagccctat ctggcgggca tcgccgccag
      361 cctgcattcc agcctgatag gccaggccgc cgatatcgag atcctggtcg aggccgaggc
      421 cctgctgatc gatccggaca aggctgtgtc ggtcggcctg atcgtcaacg agctggcgac
      481 caacgccgtc aagcacgcct tccccagcgg cggcgggcgg atcttcctgt cgttcgagcg
      541 gcgtgacgag gaggtgctgc tgatcgtcag ggatgacggc gtcggccatg accaggcccg
      601 caacagcggc gtgcgctctg gtctggggtc gcgctatgtc gaggccttcg tcaagcagct
      661 gggcgccacc atcgcccggg ccagcggcgc tggcggaacg acgatgacgg tcaagctgcc
      721 ggcctcgatc ctggtcgagg atgtcgcccc gccgcccgag gccgtcgcgc cctagaccgg
      781 cgatttcagc gtttgctcac aacttgctca tgacgctcac cgcgcatatg gggccgtatt
      841 gcggcttcac tgaaggcgcg ggagcacggt catgacggaa gatgcagagc gcaggggcaa
      901 gggcttgtgg cggattgcgg tgtggggcgg ggcgggggcc ctgctgctgg cgccgctggt
      961 ggccgtgcgc ttcaccgacg cnnnnnnnnn ncgacgccgt gaactggacc ggctcggact
     1021 tcgcgatctt cggcgtgatg ctggctgtcc ccctggcgat cctggagctg acgctccggg
     1081 cgacaggcaa cctggcctac cgggccgctg tggtcatcgc tctgggcggc gcctttctga
     1141 tgacctgggc caacctggcg gtcggccttg tgggggatga gaacaacccc ctgaacctga
     1201 tgttcttcgg cgtgctgggt gtggggctgg ccggggcggt ggtcgcgggc tttgcggccg
     1261 gcggcctggc ccgcgccatg gcggcgatgg cggtcgccca aggcctggcc ggcctggccg
     1321 ccttgatcgc gggacacgtg acaatcgtcc tgaccgggat ctttgtcctg gtctggctgg
     1381 cctcggccgg gttgttccac aaggcggcgc gccagcaggg cgcggcgccc gcgtaaaccc
     1441 cctgaacagg ggtgggcgaa cagggttctg cggctggacg gcggcggccc aatgcctcta
     1501 ggcttggcca aggttcggcg gagccacgac atatgcgggt ctcatctccc agcaccctcc
     1561 tgctgggggt ggtgttcttc tgtatcgcgg cggcgctggc ggcgatcagc ctggcgacgg
     1621 ccccggcctg gctggggctg agcgtcagcg cgtcctcggc ggcagggccg ctggtggtcc
     1681 gggacctgga ccccaggggg cctgcggcgg tcgcgggcgt caggaccgga gagcgcctgc
     1741 tggcggtgat cgctcccgac ggccgccaga cgcccctgac cggcgacatg ctcgaagaag
     1801 acgacggtgc gacccaggcc gcggccgacg ccggcatgcg ccgccatgat gtcgccgccc
     1861 aggccctgaa ggcccgcccc ctgcgcctga aggtgcagga ccccgccggg aacagccgga
     1921 ctgtcaccct ggatgagcgc cccagaccgc tttcgagcct gggcgcagag ttctgggcca
     1981 atctgtcgtt tggcctgatc gccgccctga tctgcggctg ggtgtgggcg ctgcggccgc
     2041 tggaactggc cacacagctg ttcgccctga ccggtctgtc catgctggcc ttcaccttcg
     2101 gctgggccgt gcattcagcg ctggagctgg ccatggacac cgccctgctg gggccggtgc
     2161 tgtggctgaa catggccggg gccgtggcct atggcggggc gatgattgcc tttttcctgt
     2221 gttatcccaa acgtctggcc ccggcgccgg tgctgctgct gatggccccc gtcgccgtgg
     2281 ccctgtgggc ggcaatgccg ctgctaccgg acaatcccag catttccagc atgggccagg
     2341 gcttcaccgt ggtggagatg gccctgatca tcgtcgccat cggcgtgcag accttcgcca
     2401 gtcgcaggga cgcggccgcc atggccgcca cccgctggct gggcctgtcg gtgatcatcg
     2461 gggccggcgc cttcatcggc ggggtggccg tgccgtcggc tttcggcagg cctgcgatca
     2521 tcgacccccg cttcgccctg ggcttcttcg taatcatcta tgtcggcatg gccttcgggc
     2581 tcaggnnnnn nnnnnccaca agctgttcga gctggatcag tgggcgttcc gcatcctgtt
     2641 ctatctgggc gccggtctgg ccctgctggc gctggacgcc gtgctgctga cggtgctgcg
     2701 ctacagcccg caggcctctc tgggggcggc cctgctggtg gtggccttcg cctatctgcc
     2761 gttccgcgac gtgctgtggc ggcgcacggt ggcgcggcgc acgatgaagg accacgagat
     2821 gttcgccgct gtcgcccagg tggccctgac ccatcaggcg caggcccgcg aggcggcctg
     2881 gcgcgacctg ctggccagga tctatgagcc gctggaagtg acgacggctt cgccgccgcc
     2941 gccctccgcc accatcgtcg acgagggcgt ggccatgagc ctgcccgcgg ccgccggcgc
     3001 gccggccctg ctgctgcgct tcccggcgcg gggcaggggc ctgttcggca ccgcccaggt
     3061 gcagctggcc gatcagcttg tgaccctgct gacccagatc gagcagggcc gcgacgccta
     3121 tgcccggggc gtggtcgagg agcggcttcg gatcgcccgc gacctgcacg acgatgtcgg
     3181 cgcgcgcctg ctgtcgggcc tgcaccgcaa ggagctgaac cagacccacg aggccatgcg
     3241 cggggcgatc ggtgacctgc gggccatcgt ctcgggcctg tcgggccacg agctgccgct
     3301 ggacgaggtg ctggccgatc tgcgtcacga gaccgccgag cgtctggccg ctgccggtct
     3361 ctccctggac tggccgccgc cggatctgga cgagccaagc gcgccggcgc cctatcgcgt
     3421 ctacaagaac ctgacctcgg cctgccgcga ggtgatctcc aacgccattc gccatgcggg
     3481 cgccagcaag gtcgctgtca cagtcctgca tacgaaggaa cgcctggaga tgaccatcag
     3541 cgacgacggc gtcggcctgc cgcgcgagcc gggcggacgc cgcagcggcc tggccaat
//
LOCUS       DAOMEO010000201         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2961_length_3595_cov_2.494350, whole genome shotgun sequence.
ACCESSION   DAOMEO010000201 DAOMEO010000000
VERSION     DAOMEO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_3595_cov_2.494350"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..755)
                     /locus_tag="PLH31_05875"
     CDS             complement(69..755)
                     /locus_tag="PLH31_05875"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HQR88788.1"
                     /translation="MSTVPQVEKGGYTHAVLMGDLIGSEAASSIKELHQQFNHAVDQT
                     NQLAADRLVSPLTITLGDEFQGVCRSLSDGLWIMRRVRYALLAQDVFCRFVLGVVRLE
                     TEVPSNKAWNMMGPGLSAARDRLADKKDPNVYRFQLPEHELLQSLLGAVGYAATAIEL
                     DWSSRQQQIVLASAEDKPAAQLSRELGLATRTLYKIRHSARFDVYQSQWRAMMKAAEA
                     LDHDYGLPSR"
     gene            complement(885..1319)
                     /locus_tag="PLH31_05880"
     CDS             complement(885..1319)
                     /locus_tag="PLH31_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HQR88789.1"
                     /translation="MIKYALQCDQAHLFEGWFGSSSDYDDQSARGLVDCPLCGSRTVS
                     KQIMSPAVAGTKAQKAAPAVDPKMREMMMSAMGEVRRHVEENFDNVGDAFAREARDIH
                     EGKSEERGIYGQATPAEAKALIEDGIKVAPLPPAAPKKTDMN"
     gene            complement(1339..2172)
                     /locus_tag="PLH31_05885"
     CDS             complement(1339..2172)
                     /locus_tag="PLH31_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQR88790.1"
                     /translation="MSLPESLRVGLIQTRTPATHAAALAHVTPLVREAAAQGARFILT
                     PEGTNVLQKDRTRLLPALQSLEDDIVVGGLRDLAAELGVWIDIGSALVLREDHKAANR
                     QAVIDPTGQIVATYDKLHMFDVDLPTGETARESATYEPGERAVVVETPLGIFGLTICY
                     DMRFPALYRALALNGATILTVPAAFTRPTGQAHWEILLRARAIETGSFVLAAAQGGFH
                     EDGRGTWGRSIIVGPWGEIIGLLDHDEPGVLVADLDLDAALRARAAIPALKNARAFTG
                     P"
     gene            complement(2185..2439)
                     /gene="grxC"
                     /locus_tag="PLH31_05890"
     CDS             complement(2185..2439)
                     /gene="grxC"
                     /locus_tag="PLH31_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQR88791.1"
                     /translation="MAKVTIYTRPFCGYCARALKLLGDKGADFTEIEAGMDPKLRQEM
                     MDRSGRTTFPQIFVGEQHIGGCDDMVALDRAGKLDGLLAA"
     gene            complement(2468..3250)
                     /locus_tag="PLH31_05895"
     CDS             complement(2468..3250)
                     /locus_tag="PLH31_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQR88792.1"
                     /translation="MPAEHGDFEPIWKAGPRLRGLGRGLLDLVLPPRTFEGGAAQSSG
                     LSAERWSRITFLDDPVCDGCGAAMPFDSGAPERCPLCLARPRAFARARAACVYDEHSR
                     DLILKLKHADRTDLSGLFARWLSRAAAPLLEEADAIAPVPMHPGRLLRRRYNQAAEIA
                     RPLARASGVAYLPDALIRQRDTVSQGGKSASGRRRNVAAAFVVPESRRHRVIGRRIVL
                     VDDVLTTGATAEGCARALMAAGAAQVTLAVVARVTEMQARPI"
     gene            3249..>3595
                     /locus_tag="PLH31_05900"
     CDS             3249..>3595
                     /locus_tag="PLH31_05900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR88793.1"
                     /translation="MTASPLLFDRDLLKVRLDRAAPDYASADFLKRRVAQDVVMRLET
                     ILRRFPVAVDLGSRNGAFSEALAESDAAANIDVLFQTDLSARMLGVGNGLKVVADEER
                     LPFGDATLDLAVST"
BASE COUNT          615 a         1167 c         1184 g          629 t
ORIGIN      
        1 ataggacgtt gccaacccag accagcgaga cgagagcgac cgtcgagtag gcaacggcgt
       61 ggagcatgtc agcgagacgg taggccatag tcgtgatcca acgcctccgc cgccttcatc
      121 atggcgcgcc attggctctg ataaacgtca aagcgcgccg aatggcgaat tttgtagagc
      181 gttcttgtgg ccaggccaag ctctcttgaa agttgagccg caggcttatc ttcagccgat
      241 gctagaacga tctgctgttg tctggaactc cagtccagct caatagcggt cgcggcgtag
      301 cctactgctc ccaggagtga ttgcagaagc tcgtgctccg gaagttgaaa tcgataaacg
      361 ttcggatcct ttttatccgc gagcctgtcg cgagccgccg aaagaccagg ccccatcatg
      421 ttccaagcct tgttggacgg aacttccgtt tcaagcctta cgacaccgag cacgaagcga
      481 cagaaaacgt cttgggccag cagcgcgtaa cgcacacgcc gcatgatcca caggccatcg
      541 gagagactgc ggcacacccc ttgaaactcg tcgcccagcg taatggtaag gggggaaacg
      601 aggcgatcag ctgcaagctg gttggtttga tcgacagcgt gattgaattg ctggtggagc
      661 tctttgatag acgacgccgc ttcgctgcct atcaggtcgc ccatcagtac ggcgtgggta
      721 tagccgcctt tttcaacctg tgggacggtg ctcatgctat gattgctcga cacaaatacg
      781 tcccattttc ggggcgcatt agctttgtga cccatttttg ggacacaatc aatgtgtgac
      841 ccatttttgg ggcataaatc gagaaacggc ccgctagacc gcccttagtt catgtccgtc
      901 ttcttcggcg ccgccggcgg cagcggagcc accttgatgc cgtcctcgat cagggccttg
      961 gcctcggcgg gcgtggcctg gccgtagata ccacgctcct cggacttgcc ctcgtggata
     1021 tcgcgggcct cgcgagcaaa ggcgtcgccg acattgtcga agttctcctc gacgtgccgg
     1081 cgcacttcgc ccatggccga catcatcatc tcgcgcatct tcggatcgac ggcaggcgcg
     1141 gctttctggg ccttcgtgcc ggcgacggcg ggcgacatga tctgtttgga caccgtccgt
     1201 gagccgcaca gcgggcagtc gacaagaccc cgcgcgctct ggtcgtcata gtcagacgag
     1261 gagccgaacc agccctcgaa cagatgggcc tggtcgcact ggagcgcgta cttgatcatg
     1321 tcttgaagat ggtgatggtc agggaccggt aaaggcgcgg gcgttcttga gggcggggat
     1381 ggccgcgcgc gccctcagcg cggcgtccag atcgagatcg gccacaagca ctcccggctc
     1441 gtcatggtca agcaggccga tgatctcgcc ccacggcccg acgatgatcg agcgccccca
     1501 ggtgccgcgg ccgtcctcgt gaaatccgcc ctgggccgcg gccagcacga acgatccggt
     1561 ctcgatggcc cgggcccgca gaaggatctc ccaatgcgcc tggccggtgg gacgggtgaa
     1621 ggcggccggg acggtcagga tcgtcgcgcc gttcagggcc agggcgcggt acagggcggg
     1681 aaagcgcatg tcgtagcaga tggtcaggcc gaaaatgccc agcggcgtct ccacgaccac
     1741 cgcccgctca cccggctcat aggtggctga ctcgcgcgcc gtctctccgg tcggcaggtc
     1801 gacgtcgaac atgtgcagct tgtcgtaggt cgccacgatc tggccggtcg ggtcgatgac
     1861 ggcctggcgg ttggccgcct tgtgatcctc gcgtagcacc agggccgagc cgatgtcgat
     1921 ccagacaccc agctcggcgg ccagatcgcg caggccgccc acgacgatgt cgtcctccag
     1981 gctttgcagg gcgggcagca gccgggtgcg gtccttttgc aggacgttgg tcccctcggg
     2041 cgtcaggatg aaacgcgcgc cctgggccgc cgcctcacgc accagcggcg tgacatgggc
     2101 cagcgccgcc gcgtgggtgg cgggggtccg cgtctggatc aggccgaccc gcaaggactc
     2161 tggcaggctc atccgaagag cgccctaagc tgcaaggagg ccgtccagct tgccggcccg
     2221 gtccagggcc accatgtcgt cgcagccgcc gatatgctgc tcgcccacga agatctgcgg
     2281 gaaggtggtg cggcccgagc ggtccatcat ttcctggcgc agcttgggat ccatgccggc
     2341 ctcaatctcg gtgaagtcgg cgcccttgtc gcccagcagc ttcagggcgc gggcgcagta
     2401 gccgcagaac ggacgggtat agatggtgac tttggccatg ggtgtaacga gtctctcctt
     2461 gtggtcctca tataggacgc gcttgcatct ctgtaacgcg cgccacgacc gcgagggtca
     2521 cttgcgccgc tccggccgcc atcagggccc gcgcgcagcc ctcggcggtg gctccggtgg
     2581 tcaaaacatc atcgaccagc acgatcctgc gaccgatcac ccgatgccgt cgcgactcgg
     2641 ggaccacgaa ggcggccgcg acattgcggc ggcggccaga ggccgacttg ccgccctggc
     2701 tgaccgtgtc gcgctggcgg atcagggcgt caggcagata ggcgacgccg ctggccctgg
     2761 ccaggggccg ggcgatctcg gcggcctgat tgtagcggcg gcgcagcagg cgtccggggt
     2821 gcatcggcac gggcgctatg gcgtcggcct cctccagcaa cggcgcggcg gcgcgcgaca
     2881 gccagcgggc gaacagaccc gaaaggtccg tgcgatcagc gtgcttgagc ttcaggatca
     2941 ggtcgcggga atgttcgtca tagacgcaag ccgcgcgggc cctggcgaag gccctcggcc
     3001 gcgccaggca caggggacag cgctcgggcg cgccgctgtc gaacggcatg gccgcgccgc
     3061 agccgtcgca gaccggatcg tccagaaagg tgatgcggct ccagcgctcg gccgacaacc
     3121 cgctgctctg ggccgcgccg ccctcgaagg ttcggggcgg caggacgagg tccagcagac
     3181 cccttccgag acctcgcaga cgcggtcccg ctttccatat cggttcgaaa tcgccatgtt
     3241 ccgccggcat gaccgcctcg cccctcctct tcgaccgcga ccttctgaag gtccgcctcg
     3301 accgcgctgc gcctgactac gcgtcggctg acttcctcaa gcgccgcgtg gcgcaggatg
     3361 tcgtcatgcg gctggagacg atcctgcgac gctttccggt cgccgtcgat ctgggatcga
     3421 gaaacggggc tttctcggaa gctctcgccg agagcgacgc ggcggccaat atcgacgtgc
     3481 tgttccagac cgacctgtcg gcccgcatgc tcggcgtcgg taatggcctg aaggtagtcg
     3541 ccgacgagga gcgtctgccg ttcggcgacg cgacgctgga cctggccgtc tcgac
//
LOCUS       DAOMEO010000202         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2968_length_3588_cov_2.943391, whole genome shotgun sequence.
ACCESSION   DAOMEO010000202 DAOMEO010000000
VERSION     DAOMEO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_3588_cov_2.943391"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLH31_05905"
     CDS             <1..657
                     /locus_tag="PLH31_05905"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQR88794.1"
                     /translation="VARFMLVRRVRLLDLKALAEILEKGSIFDPKYAPRKERALFLER
                     LLRRMTMPVMPSDEALEYLATQAVADFLASRIEPELDGILFPSVQVPGRKSNVVLFQK
                     AARIASIKYPKGTKLSVDTGMLNGEEWEHELTVVEEVPPPEPGKSLPPPFDQTGMGGL
                     SDIDWDSDPDSDKRPMTLDVDLTSLEVRKVTGVHFHTVAEKVDRKRIKLQRHPGRSDF
                     "
     gene            1884..2054
                     /locus_tag="PLH31_05910"
                     /pseudo
     CDS             1884..2054
                     /locus_tag="PLH31_05910"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
     gene            complement(2208..3119)
                     /locus_tag="PLH31_05915"
     CDS             complement(2208..3119)
                     /locus_tag="PLH31_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQR88795.1"
                     /translation="MSWLDRQSFLGADSDTRLAAATVGLVGLGGGNSHVVQQLAHLGV
                     GGFVLLDDDIITLTNLNRLVGATLDDVKAERPKVEIAVRVIRAVNPNARIVMRKAKWQ
                     EVGDLLMGCDIIVGGLDHIGSKDELEAFCRRFMIPYIDMGMDVTSLPGAGQSLVSGQV
                     VLSMPGEPCLRCLQLVTDERLKEEAQRYGVAGSRPQVVWPNGLLASSAVGLVVQLLTP
                     WMADPPHSAYFAYDANLGTVAPSDRLARRRGKPCSHYPAKTVGDPMFDVRRYLERLDQ
                     PAPVEPTASVPVSRLARVIHWIRTRFS"
     gene            complement(3106..>3588)
                     /locus_tag="PLH31_05920"
     CDS             complement(3106..>3588)
                     /locus_tag="PLH31_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88796.1"
                     /translation="LDDVRKDLHRPHAFAHERVGFFTAGAAGLGDRLLLTIRNYMPVA
                     DEDYEVDRKVGAKIGSAAMRKAVQSAYRPAAALLHVHAHGGRGKPGFSRVDLNSADDF
                     VPGFFETTPRMPHGLLVLSNDAAHGLLWLANDRAPVAIDQFQRIDAPLQREWGSHVLA
                     "
BASE COUNT          647 a         1142 c         1195 g          604 t
ORIGIN      
        1 gtcgctcgtt tcatgctagt gcgccgcgtt cgtctcctcg atctcaaagc cttagctgag
       61 attctcgaga aaggcagcat cttcgatccg aaatacgcac caagaaagga acgggcctta
      121 ttccttgaaa ggctgctgcg acggatgacc atgcctgtga tgcccagcga tgaagcgctc
      181 gaatatttgg cgacccaggc cgtcgctgac tttctggcgt cgcgcattga gccagaactc
      241 gacgggattc tattcccctc ggttcaggtt ccgggccgca agtctaatgt ggtgctcttc
      301 cagaaggcgg cacggatcgc gtcgatcaaa tacccgaagg gtacgaagct ctcggtagac
      361 acgggtatgt tgaacggcga ggagtgggaa cacgagctga ccgtggtcga agaagtgccg
      421 ccgcccgaac ccgggaagtc gcttccgccc cctttcgatc aaaccggcat gggcgggctc
      481 tcagatatcg attgggactc cgatccggat tcggacaaga ggccgatgac ccttgacgta
      541 gatttgacgt cgcttgaggt gcggaaggtg acgggtgtgc acttccacac ggtcgccgaa
      601 aaagtcgatc ggaaacgtat taaactccag aggcatccgg ggcgaagcga cttctgaggg
      661 aaggctgggc gaccggcgac gccaaatcgt tgcctgcgtc cgcgttgcag ttgggcgatc
      721 agggcagcga gcacgccttg cagcactcgg gtcgagggcg gccggggcta taccgatttg
      781 actgatagat cgaacgccaa cgcctgcgcg gcttccaccg gcagcggtat gcgctccgcc
      841 gccctgtcga agccgcggcg gtggggcggc tcccggaaac catcccttcc cacccaccga
      901 ctagatcctg gtccggcctg gcgggcgtga actccaacgc cttgagcggc gcgaagcccg
      961 tgcgccaacc attgcgaagg gtgatgtacg tcccgccgca ggcccggacg aaaatctccg
     1021 cgcctcgatc ttgtcgatga gatccaggct cgcgccgagt ttttcgagtt gggccaaaag
     1081 gaagttctga acgaccgtct tccccgagcc ggacggaccg cagatgaacg tgtgtccaag
     1141 atcgcctacg tggaaattga agtgggatgg agagcctgcg gaggtggcag cagcgcgact
     1201 gccgcaccgc aatggttcct attggccttt ccggcgggat gtgtatggaa cggcgcaagc
     1261 gctgcgaaat tgcgcgaggt gatcgccgcc ggccgcaccc tgcgcctgaa gttgccgaga
     1321 aactgcgccc agagcgctgc ctccaagcga gatcttcgcg ggttgtgacg atgcccgagt
     1381 ccgccaacat cgcgcgagcc ttggagacat gttcgttgag gctgcgggtg tccggccaga
     1441 agacatgccg atcgaagtcg ccaaacctga ggccgagatc agcggggacg gtgatcagcg
     1501 ccccacccca gagccggagg atgagccgga cgaagatgcg tcggcgcccc tgtcggagcg
     1561 gctggtgctg gacctgaccg cccttcggac ggctgcgctg cgcgacgccc tggtctctca
     1621 tcccgacgtg gccttggcgg tggtgcatgc gctgaccgca gcgaccttct acgcgcccga
     1681 cgagcgggcc tccttcttgg agatcaaggt gaccttcgcg cccctcgaca ggatcgcgcc
     1741 ggacattgtc gagacccgtg ccggacgcgt cgctgagcgg catgagcgct gggctgttcg
     1801 gcttcccagg gaggcggaag gcttctgggc gttcgtcgtc ggccttcccg cgacggggcc
     1861 tactacgcga ccgtcgccca tgaatgcact cactggaccc tgcaccggca cgcctagatc
     1921 gggacttcgg ccgcaagcgg tgtggcgacg aaggctatac ccgggaggag ctggtcgccg
     1981 agcttggcgc agcgttcttc tgcgcagatg tggggctgga gctgacgccc cggcccgacc
     2041 acgccagcta catcgaaagc tgggcgcggg tgctcaagga cgacaagcgg ttcatcttcc
     2101 ccgccgccgc gcaggcccaa agggcgaccg atttccggca ggccgcaggc caggcggcgt
     2161 gccccacctg gggcgagcgc ggcggcgctc gcacccttcg cgttattcta gctgaagcgt
     2221 gtgcgaatcc agtggatcac acgagccagg cgcgagaccg gaacgctggc ggtcggttcg
     2281 acgggcgccg gctgatccag gcgttcgaga tagcgacgca cgtcgaacat cggatcgccg
     2341 acagtcttgg ccgggtagtg cgaacagggc ttgccgcgcc gccgggccaa gcggtccgag
     2401 ggggccaccg tccctaagtt ggcgtcatag gcgaaatagg cgctgtgggg cgggtcagcc
     2461 atccagggcg tcagcaactg gaccacaaga ccgacggccg aggatgctaa caggccgttc
     2521 ggccagacca cctgcggccg actgccggcg acgccatagc gttgcgcctc ctctttaagc
     2581 cgctcgtcgg tgaccagctg caggcaccgc aagcaaggct cccccggcat ggacagcacc
     2641 acctggccgc tgaccaggct ttggccggcg ccgggcaaag acgtcacgtc catgcccatg
     2701 tcgatgtagg ggatcatgaa gcggcggcag aaggcttcca gttcgtcctt cgaaccgatg
     2761 tggtcgaggc cgccgacgat gatgtcgcaa cccatcaaga ggtcgccgac ttcctgccac
     2821 ttggccttgc gcatcacgat ccgggcgttc ggattgaccg cccggatcac gcgcaccgcg
     2881 atctcgacct tcggccgctc cgccttgacg tcgtctaggg tcgcgccgac cagccggttg
     2941 agattggtca gggtgatgat gtcgtcgtcg agcagaacga agccgccgac ccccaggtgg
     3001 gccaactgct ggaccacatg ggaattgcca ccgccgagcc ccaccaggcc gacggttgcc
     3061 gccgccaacc tagtgtcgct atccgcgccc aggaaactct gtcgatcaag ccaggacatg
     3121 ggatccccat tcgcgctgca ggggcgcgtc gatgcgttgg aattgatcga tggcgaccgg
     3181 cgcccggtca ttggccagcc acaaaaggcc gtgggccgcg tcgttgctga ggacgagcag
     3241 accgtgcggc atgcgcgggg tggtttcgaa aaagccgggg acgaagtcat cggcgctgtt
     3301 caggtccacc cggctgaagc ccggcttgcc gcgaccgccg tgggcgtgga cgtgcagtag
     3361 cgcagccgcc ggccggtagg ccgactgcac cgccttgcgc atcgccgccg aaccgatctt
     3421 ggccccgacc ttgcggtcga cctcgtagtc ctcgtcggcg accggcatat agttccggat
     3481 cgtcagcagc agccggtcac ccaggccggc ggcgccggct gtgaagaagc ccacgcgttc
     3541 gtgggcgaag gcatggggcc ggtgcaggtc cttgcggaca tcgtcgag
//
LOCUS       DAOMEO010000203         3588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2969_length_3588_cov_2.735635, whole genome shotgun sequence.
ACCESSION   DAOMEO010000203 DAOMEO010000000
VERSION     DAOMEO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2969_length_3588_cov_2.735635"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLH31_05925"
     CDS             <1..466
                     /locus_tag="PLH31_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HQR88797.1"
                     /translation="DRWSYVLLAEELRRVSAEPARDARELFRRMTFNALISNADDHPR
                     NHAMIAKARDWRLSPAYDLTPSTPISLERRDLALVIGDAGRIATAENLISQSPRFLLK
                     TDEATAIVAEMEQRVSSTWYDVARGAGVSAPDCELISGAFVYPGFRPEPPPP"
     gene            complement(488..1282)
                     /gene="radC"
                     /locus_tag="PLH31_05930"
     CDS             complement(488..1282)
                     /gene="radC"
                     /locus_tag="PLH31_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQR88798.1"
                     /translation="MVRRWLLEASMPRPSHRNDAKPSQTADLFAAAPGVGEMTARYTD
                     PSSPAPANGTLGARAATGGLAGLSDAEALELLLSRSLANGARATAVALLARFGCLRRV
                     LTADLAALSAFVDTRVGVDLQLIYDAARRLAAAELPGRCVVSSWTSLIDYLKLTMAHC
                     DREAFRVLFLDKKNQLIADEILNHGTIDHAPVYPREVVRRALEVSASAVILVHNHPSG
                     DQTPSSADIEMTKKVIDAARTLGIAVHDHVIVGRAEVASFKALGLI"
     gene            complement(1397..2293)
                     /locus_tag="PLH31_05935"
     CDS             complement(1397..2293)
                     /locus_tag="PLH31_05935"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HQR88799.1"
                     /translation="MSNPLEPLLNEIIARLERGVPPWRQPWANGADPSTPLRSDGQPF
                     SGSNAWLLAFAGAERGYTSPFWFTFRQALAIGAPVAKGARSALAILYKTRVVDGRDVA
                     DSSEAGEGEAKTLRYLKTYAVFNAEQLTDCPQIYLEAPKIDPAVRAAARDAVLDAIPA
                     RLEIGGNTACYVRAADLIRMPPPEAFGCVEDWKSTFAHEAVHWSGAEHRLGRTFGQRF
                     GDAAYAFEELVAEIGSAAIGLRIGLRPQILDSHAAYLGHWVKILKDRPGALLEASGHA
                     QRAVDHLLAYSQPAQASQPLAA"
     gene            complement(2441..>3588)
                     /locus_tag="PLH31_05940"
     CDS             complement(2441..>3588)
                     /locus_tag="PLH31_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HQR88800.1"
                     /translation="DNLESPWAHRALQAFYVALHWGNVPAMDRVTFEQKTGLQLLDSD
                     GQLKKAEDLPPMNTWLNRLLALRIEDQNALFETFDAVLASILERAAASGALDKGMEDI
                     VADDLTVTSEEVIRTDAVSGAQTKVVTFAVRTRRVLASAADALAGLDPQTLEYVVNTK
                     SQRAGLVVKGLTTTDDDDRLVQAVRLIRAEKRTVLPLKTYEESAWEAVAEPIWRATWD
                     AEVAGADPWHTRQLALVTGLLLPVWSSLPSKRTFVRRLKAPDGRRWLGRVLDPTDVTK
                     LKIALGISDVATAVGSGDNAASMVLGENSSIALAGGFWLRRAKVMDRYRLEVVGAGSQ
                     RAMFQALGCFVEIINYTPRVFVPVDQPQVLSAVLAKWPAQTILPAAA"
BASE COUNT          575 a         1254 c         1207 g          552 t
ORIGIN      
        1 ggatcgctgg tcctatgtgc tgctggccga ggaactgcgc cgggtcagcg ccgagcccgc
       61 cagggacgcg cgcgagcttt tccgccgcat gaccttcaac gccctgatct ccaacgccga
      121 cgatcacccg cgcaatcacg cgatgatcgc caaggccagg gattggcggc tgtccccggc
      181 ctatgacctg acgccatcga ccccgatcag ccttgaacgc cgcgatctgg cgctggtgat
      241 cggagatgcc ggccgcatcg ccacggcgga aaacctgatc tcacaaagcc cacgctttct
      301 gctcaagacg gacgaggcca ccgccatcgt cgcagaaatg gagcagcggg tgtcgagcac
      361 ctggtacgac gtcgcccgcg gcgccggcgt gagcgcgccc gactgcgagc tgatctcggg
      421 agccttcgtc tatccgggct tccggcccga accgcctccg ccctgaccgt gatcaaggcc
      481 gccgcagcta gatcaacccc agagccttaa agctggcgac ctcggcgcgc ccgacaatga
      541 cgtggtcatg caccgcgata cccagggtcc gcgcggcgtc gatgaccttc ttggtcatct
      601 cgatgtcggc cgacgagggg gtttgatcgc ccgatgggtg attatggacc aaaatcacgg
      661 ccgacgcgga cacctccagc gccctccgca cgacctcgcg cggatagacg ggcgcgtggt
      721 cgatggtgcc gtgattgagg atctcatcgg cgatcagctg gttcttcttg tcgaggaaca
      781 ggacgcggaa cgcctcgcgg tcacagtggg ccatggtgag cttcaggtag tcgatgagcg
      841 acgtccacga ggagacgacg caacgccctg gcaactcggc cgccgcgagc cggcgcgcgg
      901 catcgtagat cagctgcagg tcgacgccga cgcgagtgtc gacgaacgcc gaaagcgcgg
      961 caaggtcggc cgtcagcacc cgccgaagac aaccgaaccg ggccagaagt gctacggccg
     1021 ttgcccgggc gccgttggcc agggaccgag agagcaggag ttcgagcgcc tcggcgtcgc
     1081 tcaggcccgc caggccgccg gtcgcggccc tcgcgcccag cgtcccgttt gcgggggcgg
     1141 gagacgaagg gtcggtgtag cgagcggtca tctcgcccac gcccggcgcc gcggcgaaca
     1201 gatcggcggt ctgggaaggt ttggcgtcgt tacggtgaga aggtctgggc atggaagcct
     1261 caagcagcca gcgccggacc aaatcccggt tcgctcgcga gctcccagcc cttcctccga
     1321 ctttcccatc gggccgggca gggcgggcgc cgaaccggcc aagcctcgcc acaccggggg
     1381 gaggggcccg gcgcccctac gcggccaggg gttggctggc ctgcgccggc tggctgtagg
     1441 cgagcagatg atcgacggcc cgctgggcgt gcccggaggc ttccagcagg gccccgggcc
     1501 ggtccttcag aatcttgacc cagtgaccga gatacgccgc gtggctgtcg aggatctggg
     1561 gccttagccc gatccgcagc ccgatcgccg cactgccgat ttcggcgaca agctcctcga
     1621 acgcataggc ggcgtcgccg aaccgctggc cgaaggtgcg gcccaggcga tgctccgcgc
     1681 cgctccagtg gacggcctcg tgagcaaagg tggacttcca gtcctccacg catccgaacg
     1741 cctcgggcgg cggcatccgg atcaggtcgg ccgcgcgcac atagcaggcg gtgttgccgc
     1801 cgatctccaa cctggccggg atcgcatcga gcaccgcatc gcgcgccgcc gcccgcacgg
     1861 ccgggtcgat cttcggcgct tcgaggtaga tctgcggaca gtctgtcagc tgctcggcgt
     1921 tgaacaccgc gtaggtcttc agatagcgca gggtcttggc ttccccctcg ccggcctcac
     1981 tggaatcggc gacgtcgcgg ccatcgacga cccgggtctt gtagaggatc gccaacgcgc
     2041 ttctggcgcc cttggcgacc ggcgcgccga tcgccagcgc ctgtcggaag gtgaaccaga
     2101 aggggctcgt atagccgcgc tccgcgccgg cgaacgccag aagccaggcg ttggacccgc
     2161 tgaacggttg gccgtcggac cgcaggggcg tggacgggtc ggccccgttc gcccagggct
     2221 gtcgccaggg cggcacgccg cgctccaggc gggcgatgat ttcgttcaac agcggctcaa
     2281 ggggattgga catcaggtcc tccggcggtt tcgcgccgga tccattcccg gtcgctcaac
     2341 cctccggtcc cttcctccgc ctttcccccc gcgccgccgt cgcccccgtc gcatcacgca
     2401 aaaagagccg caggcgcaag cccacggctc ttgatgacct ctacgccgcc gccggcagga
     2461 tggtctgggc tggccacttg gccaggacgg ccgagagcac ctgtggctgg tcgacaggca
     2521 cgaagacgcg aggggtgtag ttgatgattt cgacaaagca gccgagcgcc tggaacatcg
     2581 cgcgctggga gccggcgccg acaacctcaa gccggtagcg gtccatcacc ttggcccggc
     2641 gaagccagaa tccgcccgcc agggcgatgg agctgttctc gcccaggacc atactggccg
     2701 cgttgtcgcc ggagccgacg gccgtggcga cgtcgctgat cccgagcgcg atcttcagct
     2761 tggtgacatc cgtggggtcc agaacccgcc caagccagcg ccgcccgtcc ggagccttca
     2821 gccggcggac gaaggtccgc ttgctgggca gactgctcca caccggcagc agcagtccgg
     2881 tgaccagggc cagctggcgc gtgtgccagg gatcggcgcc ggccacctcg gcgtcccagg
     2941 ttgcgcgcca gatgggttcg gcgacggcct cccaggccga ttcctcatag gtcttcaggg
     3001 gcagcacggt gcgcttctcg gcgcggatca gccggacggc ctgaaccaga cggtcatcgt
     3061 cgtcggtcgt cgtcaggccc ttcaccacca ggccggcgcg ctgcgacttg gtgttgacca
     3121 catactccag cgtttgcgga tcgagcccgg ccagcgcatc cgccgccgac gccagcacgc
     3181 ggcgcgtgcg gacggcgaag gtcacgacct tggtctgcgc cccggacacc gcgtcggtcc
     3241 ggatcacctc ctccgacgtc accgtcaggt cgtcggcgac gatgtcctcc atgcccttgt
     3301 ccagagcgcc cgaggcggcg gcccgttcga ggatgctggc gagcaccgcg tcgaaggtct
     3361 cgaacaaggc gttctgatcc tcgatccgca gcgccaggag ccggttgagc caggtgttca
     3421 tcggcggcag gtcctcggcc ttcttcaact ggccatcgct gtccaggagc tgaagcccgg
     3481 tcttttgctc gaaggtcacc cggtccatcg ccggcacgtt cccccaatgc aaggcgacgt
     3541 agaaggcctg cagcgcgcgg tgcgcccaag ggctttcgag attgtcct
//
LOCUS       DAOMEO010000204         3580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2975_length_3580_cov_2.609078, whole genome shotgun sequence.
ACCESSION   DAOMEO010000204 DAOMEO010000000
VERSION     DAOMEO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_3580_cov_2.609078"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1447..2922)
                     /locus_tag="PLH31_05945"
     CDS             complement(1447..2922)
                     /locus_tag="PLH31_05945"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQR88801.1"
                     /translation="MSVLHDPAERDRLALKRPDLLREAAWINGAWVTASASIAVTNPA
                     TGRVLGHVPSLGEGETQEAVAAAEAAFPPWAARAAKERAAILRRWFELIMAHRDDLAR
                     LMTAEQGKPLAEAQGEVAYAAGFIEWFGEEAKRAYGEVIPGHQADKRIIVLKQPVGVV
                     AAITPWNFPAAMITRKVGPALAAGCTVVLKPSELTPFSALALALLGAEAGLPAGVFNI
                     VTGPPAPIGAVLTTDPRVAKFTFTGSTAIGKMLAAACMGTVKRVSLELGGNAPFIVFD
                     DADLDAAVEGAIASKFRNTGQTCVCANRLLVQAGVYDAFAARLTDRVKALRVGDGLEG
                     PTDQGPLINSAAVEKVERHIADAVACGARLLTGGARRGETGALFEPTVLAEVPVNALL
                     NREETFGPVAGLVRFETEAQALAIANDTRAGLASYVFTRDLNCAWRMGEGLQYGMVGL
                     NTGMISTEVAPFGGVKESGLGREGSHFGLDEYLELKMMCIG"
     gene            complement(2919..>3312)
                     /locus_tag="PLH31_05950"
     CDS             complement(2919..>3312)
                     /locus_tag="PLH31_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR88802.1"
                     /translation="TYAGPPIACAAALAVLDVIADEKLMERADAQGARIRGRLAALSQ
                     RNDLVPVANIRGPGAMVGFDILRSRDGEPDGAEAKAVTVRALTKGLILLSCGVYGETI
                     RLLAPLTASDKILDEGLDILEAALERQS"
     assembly_gap    3313..3322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3323..3545)
                     /locus_tag="PLH31_05955"
     CDS             complement(<3323..3545)
                     /locus_tag="PLH31_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR88803.1"
                     /translation="MLKALRSLCDRHGILLISDEVQAGFARTGKMFGIEHSGVQPDLI
                     TVAKSLAGGFPLSGVIGKAAIMDAAEPGGL"
BASE COUNT          662 a         1199 c         1128 g          581 t
ORIGIN      
        1 ggggcagcga gggcggcaag gcagacgcca gccataagcg cggccgtacg gccgttgtgc
       61 gaacgagaca tgaggaacct ttgatctgat gtgcaggcgc gcggccgctg tctgcggcgc
      121 gcttgggagg ggccgtaaga atcagatctg caggggtggt ccggtggatg gcggcggggg
      181 cgcggcgagc ccgcggcctg gcgttgtttg cggtgcaaca ggaatgggcc gccgcgccca
      241 gcgccagtcc accatgacgg cgagcaaggg agcctcggcc gcaacaccgg cgttgaagac
      301 cgagaaggcg caagaggggt cgctcttctg atcgtctgga tgctcgaccg gagcgtctga
      361 ggggcgttgg gcgccatagc ccgccgcttc agagtttatg gtccgggcgc cctgcgatgt
      421 gcagagcacg atcggaaatg agccagcttg cgggctcgcg gccaccatat agccctgcgg
      481 gatgagcacg cgtagggcga ttgccacgca ggccagcagc accgcaaagg ctggcgccct
      541 gactcccctc ttactcaatc cgggcattgc gtcgctgact ggcatccaag tcccgaaagt
      601 ccacaccgct gctacagtcg ggcgtatgcc cacccggcta cagagccgaa acaggcccat
      661 ggcggcgtcc aaggaccaag ccgacggcct ccacgaccat tcctgcaaat acagcggtga
      721 cgagcttgag catgcgcttg tcctaggaag agtgggctgc gtgggcctag cgccggagca
      781 tccttgcgaa gaaaccagcg ccctcaccga cgtgaccttc acaacgctcc gccttcggca
      841 cgccccgcag cacttcttcg acgtggcgac cgcaaccgac ccaacccatc ttgccgcatt
      901 tcccgcaacg aaccgctcgg cacatggcaa tcgctcctgt cagtttcaaa tttcatatat
      961 tcgcatatta gataacgtca atataaacaa acagacgacg ccccgcttgc ggccggccgc
     1021 tagagcattt tccgataagt gtgaaacggt tatcggatcg aaaaatgctc taaacttttg
     1081 atttagagcc cttttcgccc gaccctgatg aagccatcag gtcggaaagg gctctagcgt
     1141 cgcgacacct gggctgggct gaaacgcttt gcgacgtcag gagcgcgagc taggcctgat
     1201 acctgcgaga cgaccaggct ggcgcctgca ccccagccag tttagtcggg cggcccgacc
     1261 atgacgatct cggcctgccg caacctgact tgaaagcgcg ccgtttccca gctccgccgc
     1321 cggttcggcg tcaaaggcca cctcgatatg aaactcgggc gcgatcaacc ttgtgggtga
     1381 aagcctcatg ccacgcctcc agcgtgatct ggaggtatgt gcgcatgagc agatcatctg
     1441 cccctcctat ccgatgcaca tcatcttcag ttcaagatac tcgtcgaggc cgaagtggga
     1501 cccctccctg ccgagcccgg attccttgac tccgccgaac ggcgccactt cggtcgagat
     1561 catgccggtg ttcagaccaa ccatgccata ttgcaggccc tcccccatcc gccaggcgca
     1621 gtttagatcg cgcgtaaaga cgtaagaggc caatccggcc cgcgtatcgt tcgcgatggc
     1681 cagggcctgg gcttcggtct cgaaccgcac caatccggct acaggtccga aggtctcctc
     1741 gcggttcagc agcgcattaa ccggcacctc ggccaagacc gtcggctcga acagggcgcc
     1801 ggtctcgccc cggcgcgcgc cgccggtcag aagccgggcg ccgcacgcca cggcgtcggc
     1861 gatatgacgt tcgacctttt caaccgccgc gctgttgatc aacggcccct ggtcggtcgg
     1921 tccctcaagg ccgtcgccga ccctcagcgc cttgacccgg tccgtcagcc gggcggcgaa
     1981 ggcgtcatag acgccggcct ggaccagcaa acggttggcg cagacgcatg tctggccggt
     2041 gttgcggaac ttcgaggcga tggcgccctc gacagccgcg tctaggtccg cgtcatcgaa
     2101 aacgatgaac ggcgcattgc cgcctagctc aagcgagacg cgcttgaccg tgcccataca
     2161 ggcggctgcc agcatcttgc cgatggcggt agaacccgtg aaggtgaact tggccacacg
     2221 ggggtcagtg gtcagaaccg cgccaatcgg ggccggtggt ccggtgacaa tgttgaaaac
     2281 gccggcaggg agccctgctt cagcgcccaa cagggccagg gccaaggccg aaaacggtgt
     2341 caactccgat ggcttgagca ccacggtgca gccggcggca agggctggcc cgaccttgcg
     2401 agtgatcatc gcggccggaa agttccacgg cgtgatggcg gcgacgacgc ccacgggctg
     2461 cttgagtacg atgatccgct tgtcagcctg atggcccgga atcacctcgc cataggctcg
     2521 cttcgcctct tcgccgaacc actcgatgaa ccccgcagca taggccacct cgccctgggc
     2581 ctcggcgaga ggcttgccct gttcggcggt catgagccgg gccagatcgt cccgatgggc
     2641 catgatcagc tcgaaccagc ggcgcagaat tgcggccctc tccttggcgg cccgggccgc
     2701 ccaaggcggg aaggccgcct ccgcggcggc gacggcctct tgagtctccc cttcacccag
     2761 ggagggaaca tggcccagca ccctgcccgt cgccggattg gtcacggcga tagaggctga
     2821 agcggtcacc caggcgccgt tgatccaggc ggcctcccgc aggaggtcgg gacgcttcag
     2881 cgccagacgg tcgcgttcgg cgggatcgtg gagaacgctc acgactgacg ctccagggcc
     2941 gcttcgagga tatccagacc ctcatcgaga atcttgtccg aggctgtcag gggcgccagc
     3001 aggcggatcg tttcgccata aaccccgcag gacagcagga tcagcccttt ggtgagcgcc
     3061 cgcaccgtga ccgccttggc ctcggcgcca tccggctcgc cgtcgcggga acgcaggata
     3121 tcgaagccga ccatcgcccc cgggccccgg atattggcga ccggaaccag gtcattgcgc
     3181 tgactcaggg ccgcgagccg cccacggatg cgggcgccct gagcgtcggc gcgttccatc
     3241 agcttctcat cggcgatcac gtccagaacg gccagggccg cggcgcaggc gatcggcgga
     3301 ccggcatagg tgnnnnnnnn nnccaggccg ccaggctcag ccgcgtccat aatcgccgcc
     3361 ttgccgatca cgccggacag ggggaaacca ccggccagtg acttggccac ggtgatcagg
     3421 tccggctgca cgccgctgtg ttcgatgccg aacatcttgc ccgtgcgggc aaaccccgcc
     3481 tgaacctcgt ccgaaatcag caggatgccg tggcggtcgc agaggctgcg cagcgctttc
     3541 agcaactccg gcggagcggg ctggaagccc ccctctccct
//
LOCUS       DAOMEO010000205         3569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_2988_length_3569_cov_2.426864, whole genome shotgun sequence.
ACCESSION   DAOMEO010000205 DAOMEO010000000
VERSION     DAOMEO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_3569_cov_2.426864"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..648)
                     /locus_tag="PLH31_05960"
     CDS             complement(43..648)
                     /locus_tag="PLH31_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HQR88804.1"
                     /translation="MPQSNRLARWVLIASILAGVSYVASWDRGLPQAAELTWKGLGVS
                     LLAVYAALKARDLDGWLLVAVMAFGALGDVLLGAMGLTVGAIAFLAGHVVAIGLYLRN
                     RRRPLSRSQLAFAVVLVPATVIIAYLLPSDRAAAPGVAFYSLGLSLMAAAAWTSRFHR
                     FRVGIGAVMFVVSDLLIFGRAGPLPDNFVTGLGVWGLYLYL"
     gene            786..2327
                     /locus_tag="PLH31_05965"
     CDS             786..2327
                     /locus_tag="PLH31_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HQR88805.1"
                     /translation="MNGADALITTLADNGVTACFANPGTSEMQFVAALDREPRMRSIL
                     CLFEGVATGAADGYGRMAGKPACTLLHLGPGYANGAANLHNARRAYTPVVNVIGDHAT
                     DHRQYDAPLNSDIAALAAPNSIWVKSAESADQVGALAAEAVAASFGAPGGNACLILPA
                     DSAWNETNAAGPVIAAPRPAAPDASAVAAVAAAIKAADKPTILLGSSACGEAGIAAAG
                     RLAAVGVRMLTDTFTARQSRGEGRFRPDKMMYFGEQALKDLAGTDLIVLAGTSQPVAF
                     FAYPDRPSVLVPDGCALETLCGREVDAPAALNALADALGAPVEGAVEAYVEPAAPGPK
                     FDAWAMGASIARHMPADAIISDDAVTAGLPIFTQTKNARAHDWLSLTGGAIGQGIPVA
                     IGAAVACPDRKVLSLNGDGAGMYTVQGLWTIAREKLDVTVVIFANGAYRILGIEMGRT
                     GGGEPGPKASRLLDLGDPKIDWVALATGLGMPAERVETSEAFDAAMAYAMTTPGPHFI
                     EAAMR"
     gene            2334..3011
                     /locus_tag="PLH31_05970"
     CDS             2334..3011
                     /locus_tag="PLH31_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQR88806.1"
                     /translation="MTAEPAHLPLPDRWDLTDQQMLDRADAALARLRRRHSIRDFSDR
                     PVPQAVVERCIAAAATAPSGANQQPWRFVLIGPGPLRTALREAAEAEEREFYAGRAGE
                     AWLGALAPLGTDADKPFLETAPWLIAIFGQRHGVDPDGGKIKHYYVPESVGIATGFLI
                     AALNEAGLATLTHTPSPMGFLNTALGRPEHEKPYILLVVGHPAEGATVPAHAKVKLPL
                     DEVMSLR"
     gene            complement(3094..>3569)
                     /locus_tag="PLH31_05975"
     CDS             complement(3094..>3569)
                     /locus_tag="PLH31_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88807.1"
                     /translation="LYSRLATKGGFDCDVEKYGMLPSADPKKEIVELKCKNRTDGAIA
                     IFSTGPAVIYDCLFAELNGYRCTFSKQDSLFPRLSADLKAYGKGSCEVSGVRLVGRNE
                     TDGYFEVACSDGLPGWVMSYPVNTPAPKSKEILSCSQAKGIGGGCKLPTNASKKA"
BASE COUNT          574 a         1249 c         1223 g          523 t
ORIGIN      
        1 ttaaaccagt tttcgcttgc gtgagcctgg tttttgggcg tattacagat aaaggtacag
       61 cccccacacg cccagtccgg tgacaaagtt gtccggcagc gggccggcgc ggccgaagat
      121 cagcaggtcg gagaccacga acatcaccgc cccgatcccg acgcggaagc ggtggaagcg
      181 gctggtccag gccgcagccg ccatcagcga caagcccagg ctgtagaacg ccacacccgg
      241 cgcggcggcg cggtcggacg gcagcaggta agcgatgatg accgtggccg gaaccagcac
      301 gacggcgaac gccagctggc tgcgcgacag gggccggcgc cggttgcgca gataaaggcc
      361 tatggcgacc acgtgtccgg ccaggaaggc gatggcgccc accgtcaggc ccatggcccc
      421 cagcagcacg tcgcccaggg cgccgaaggc catcaccgcg accagcagcc agccatccag
      481 atcgcgcgcc ttcagcgccg catagacggc cagcaggctg acgcccaggc ccttccaggt
      541 cagctcggcg gcctggggca ggccccggtc ccagctggcg acatagctga ccccggcgag
      601 gatcgaggcg atcaggaccc accgggccag acggttggat tgcggcatat agtttccccc
      661 ggccggcgcg ttctttggcg acgcttcggc gggtccagac tgcgtcaagc gacccgaccg
      721 acgcaaccac cgttggcgcc tcccgcgaaa tgtcggaaac aggggcacac ggtacggaga
      781 agaccatgaa cggcgcggac gccctgatca ccaccctggc cgacaacggc gtaaccgcct
      841 gtttcgccaa tcccggcacc agcgagatgc agttcgtcgc ggcgctcgat cgcgagccgc
      901 gcatgcgctc gatcctgtgc ctgttcgagg gcgtggccac tggcgccgcc gacggctatg
      961 gccgcatggc cggcaagccg gcctgcaccc tgctgcatct gggtcccggc tacgccaatg
     1021 gcgcggccaa tctgcacaat gcccggcggg cctatacgcc ggtggtcaat gtgatcggcg
     1081 accacgccac tgatcaccgc cagtacgacg cgcccctgaa ctccgacatc gcggcgctcg
     1141 ccgcgcccaa ctcgatctgg gtgaagtccg ccgagagcgc cgatcaggtc ggggccctgg
     1201 ccgccgaggc cgtggccgcc agcttcggcg cgccgggcgg caacgcctgc ctgattctgc
     1261 cggccgacag cgcctggaac gagaccaacg ccgccgggcc ggtgatcgcg gcgcctcgcc
     1321 cggccgcgcc cgacgcctcg gccgtggctg ccgtcgccgc tgcgatcaag gcggcggaca
     1381 agccgaccat cctgctgggc tccagcgcct gcggcgaggc ggggatcgcc gctgctggac
     1441 gcctggccgc tgtgggcgtg cggatgctga ccgacacctt caccgcccgt cagtcgcgcg
     1501 gcgaggggcg cttccgccct gacaagatga tgtatttcgg cgagcaggcc ctgaaggacc
     1561 tggccggaac cgacctgatc gtcctggctg gcacaagcca gccggtggcc ttcttcgcct
     1621 atcccgaccg gcccagcgtg ctggtccccg acggctgcgc gctggaaact ctgtgcggtc
     1681 gcgaagtcga cgcccccgct gccctcaacg ccctggcgga tgctttgggc gcgcccgtcg
     1741 agggggcggt cgaggcctat gtcgaacccg ccgcgcccgg tcccaagttc gatgcctggg
     1801 ccatgggggc ctcgatcgcc cggcacatgc cggccgatgc gatcatctcc gacgatgcgg
     1861 tcaccgctgg cctgccgatc ttcacccaga ccaaaaacgc tcgcgctcat gactggctgt
     1921 cgctcaccgg cggcgcgatc ggccagggca ttccggtcgc gatcggcgcg gccgtggcct
     1981 gtcccgatcg caaggtgctg agcctgaatg gcgacggcgc ggggatgtac acggtgcagg
     2041 gcctgtggac gatcgcccgc gagaagctgg acgtcaccgt ggtgatcttc gccaatggcg
     2101 cctatcgcat cctgggcatc gagatgggcc gcaccggcgg cggcgagccg gggccgaagg
     2161 cctcgcggct gctggacctg ggcgatccga agatcgactg ggtggcgctg gcgacagggc
     2221 tgggcatgcc ggccgagcgg gtcgagacat ccgaggcgtt tgatgcggcc atggcgtacg
     2281 cgatgacgac gccggggccg cacttcatcg aagcggcgat gcgataggcg atcatgaccg
     2341 ccgagcccgc ccacctgccc ctgccggacc gctgggacct gaccgaccag cagatgctgg
     2401 atcgcgcgga cgcggccctc gctcgcctgc gtcgccgcca tagcatccgc gatttcagcg
     2461 atcgccccgt gccgcaggcg gtggtcgagc gctgcatcgc ggcggcggcc accgcgccca
     2521 gcggggccaa ccagcagccc tggcgcttcg tgctgatcgg cccgggtccg ctacgcacgg
     2581 cccttcgcga agcggccgag gccgaggagc gggaattcta cgctggccgg gcgggcgagg
     2641 cctggctggg cgccctggcg ccgctgggca ccgacgccga caagcccttc ctggagacgg
     2701 ccccctggct gatcgccata tttggccagc ggcatggcgt cgaccccgac ggcggcaaga
     2761 tcaagcatta ctacgtgccc gaatcggtgg gcatcgcgac gggcttcctg atcgcggcgc
     2821 tgaacgaggc gggcttggcg accctgaccc acacgcccag ccccatgggc tttctcaata
     2881 ccgccctggg gcggccggag cacgaaaagc cctacatcct gctggtggtg ggtcaccctg
     2941 ccgagggcgc gaccgtgccg gcgcacgcca aggtcaagct gcccctcgat gaggtgatga
     3001 gccttcgctg aggccgagcg ctcaacgcca gaccataaaa aagcccgccg ggatcgctcc
     3061 cggcgggctt gctgtttcag gcttgcgccg tgatcaggct ttcttggagg cgttggtcgg
     3121 cagcttgcag ccgccgccga tacccttggc ctgcgagcag gagaggatct ccttggactt
     3181 cggggccggg gtgttgaccg gatagctcat cacccagccg ggcaggccgt ccgagcaggc
     3241 gacttcgaaa tagccgtcgg tctcgttgcg gccgaccagg cggacgcccg agacttcgca
     3301 cgagcccttg ccataggcct tcagatcggc cgacaggcgc gggaacagcg agtcctgctt
     3361 ggagaaggtg cagcgatagc cgttcagttc ggcgaacagg cagtcataga tcaccgccgg
     3421 accggtcgag aagatcgcga tcgcgccgtc agtgcggttc ttgcacttca gctcgacgat
     3481 ttccttcttg gggtcggcgc tgggcagcat gccgtacttt tcgacgtcgc agtcgaagcc
     3541 gcccttggtg gccagccggc tgtaaaggc
//
LOCUS       DAOMEO010000206         3546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3002_length_3546_cov_2.316528, whole genome shotgun sequence.
ACCESSION   DAOMEO010000206 DAOMEO010000000
VERSION     DAOMEO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_3546_cov_2.316528"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="lepA"
                     /locus_tag="PLH31_05980"
     CDS             complement(<1..1186)
                     /gene="lepA"
                     /locus_tag="PLH31_05980"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQR88808.1"
                     /translation="MTSTPLDKIRNFSIVAHIDHGKSTLSDRLIQETGGLTAREMTAQ
                     VLDNMEIEKERGITIKAQTVRLEYKADDGETYILNLMDTPGHVDFAYEVSRSLAACEG
                     SILVVDASQGVEAQTLANVYSAIDNNHEIVPVLNKIDLPAAEPERVRAQIEDLIGLDA
                     SDAVLCSAKSGIGIHEVLEAVVTRLPPPKGDENAPLKALLVDAWYDAYLGVVVLVRVI
                     DGRLRAGQKIKMMQHGSTHLVDRVGVFKPKNTPVESLGPGEIGFFTAQIKEVADAAVG
                     DTLTEEKRPTEHALPGFKDVQPVVFCGLFPVDAADFEDLRAAVGRLRLNDGSFTYEME
                     SSAALGFGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVVYKIHMRDGSEIELHNP
                     ADL"
     gene            1346..1972
                     /locus_tag="PLH31_05985"
     CDS             1346..1972
                     /locus_tag="PLH31_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HQR88809.1"
                     /translation="MDRRKLILSGAATLGASVGASSVFAQDTMSQDVASSGTTTPAYD
                     TGSAQTYSADEMIRATSDFLGVTAESAGAAIEKVFRDKGQPTGYIAGEEGSGAIGVGL
                     RYGRGLLYMKGRPTLETFWQGPSVGWDWGGNASRAFTLCYNLQYPDAIFRRFPGVEGT
                     AYLVGGLGVNYQKADDIVLAPIRAGVGLRLGANVGYLGYSRKRRIMPF"
     gene            complement(1991..2498)
                     /locus_tag="PLH31_05990"
                     /pseudo
     CDS             complement(1991..2498)
                     /locus_tag="PLH31_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
     assembly_gap    2638..2647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2648..>3546)
                     /locus_tag="PLH31_05995"
     CDS             complement(<2648..>3546)
                     /locus_tag="PLH31_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88810.1"
                     /translation="SDKPSLSTLIDVPVATTAKLTLSYLAIGVNWQADYVARIAPDGK
                     TLDLTGWLTLVNSSGTRFVDAPTQAVAGKLSRVPVNIPRADTEAITRACWPMDTTTHG
                     RPPPPPPPPLPIPPVMMSMAPAGAAREEITVTAQRAKMAEQSELGDYKLYTLPEPVTL
                     AARQTKQVAFLDQKGVAFQRLYMVTVDPWSDYGDDNLPAATVLLRLENKANQGLGKAL
                     PSGALAVMETASGRPAFAGEQTMRDVPVGQPFDLAIGRAMDVSVRPRLVRETRASRGR
                     VRRSYEIDLANAKSGSITIELRH"
BASE COUNT          582 a         1091 c         1249 g          614 t
ORIGIN      
        1 gcaggtcggc ggggttgtgc agctcgatct cgctgccgtc gcgcatgtgg atcttgtaga
       61 ccaccgaggg ggcggtcgcg atcaggtcga ggtcgaactc gcgcgacagg cgctcctgga
      121 tgatttccag gtgcagcagg ccgaggaacc cgcagcggaa gccaaagccc agggcggcgc
      181 tgctttccat ctcgtaggtg aagctgccgt cgttcaggcg caggcggccg acggcggcgc
      241 gcaggtcttc aaagtcggcg gcgtcgaccg ggaagaggcc gcagaacacg acgggctgca
      301 cgtccttgaa gccgggcagg gcgtgctcgg tggggcgctt ttcttcggtc agggtgtcgc
      361 cgacggcggc gtcggccact tccttgatct gggcggtgaa gaagccgatt tcgccggggc
      421 cgaggctctc gacgggggta ttcttgggct tgaagacgcc gacgcggtcg accaggtggg
      481 tcgagccgtg ctgcatcatc ttgattttct ggccggcgcg caggcggccg tcgatgacgc
      541 gcaccagcac caccacgccc agataggcgt cgtaccaggc gtcgaccagc agggccttca
      601 ggggcgcgtt ctcgtcgccc ttgggcggcg gcaggcgggt gacgacggct tccagcacct
      661 cgtggatgcc gatgccggac ttggcgctgc acagcaccgc gtcgctggcg tccaggccga
      721 tcaggtcctc gatctgggcg cgcacacgct cgggctcggc ggcgggcagg tcgatcttgt
      781 tgaggaccgg gacgatctcg tgattgttgt cgatggccga atagacattg gccagggtct
      841 gggcttccac gccctggctg gcgtcgacga ccaggatcga gccttcgcag gcggccaggg
      901 agcgcgagac ttcgtaggcg aagtcgacgt ggccgggggt gtccatcagg ttgaggatat
      961 aggtctcgcc gtcgtcggcc ttgtactcca gacgcacggt ctgggccttg atggtgatcc
     1021 cgcgttcctt ctcgatctcc atgttgtcga gaacctgggc ggtcatttcg cgcgcggtca
     1081 ggccgccggt ctcctggatc aggcgatccg acagcgtgga cttgccgtgg tcgatgtggg
     1141 ccacgattga aaaattacgg atcttgtcga gcggcgtaga agtcatgcgc ccgcgtctag
     1201 cacatttggg ccgcttcgcg agggggcgat gcgcctgcgg cggggctgga tttccccatg
     1261 cttaaccgaa ccttaaagcg accggccgca aaccggtccc aatgggctgc gacagtagcg
     1321 caacgatatt tctgaagggg ccgtcatgga ccgtcgcaaa ctgatcctct cgggcgcagc
     1381 cactctgggc gccagcgtcg gcgcctccag cgtcttcgcc caggacacga tgagccagga
     1441 cgtggcctcc agcggcacga cgaccccggc ttatgacacg ggctcggccc agacctattc
     1501 ggccgacgag atgatccgcg ccacgtccga cttcctgggc gtcacggccg aaagcgccgg
     1561 cgccgccatc gagaaggtgt tccgcgacaa gggtcagccc accggctaca tcgccggcga
     1621 agagggctcg ggcgccattg gcgtgggcct gcgctatggt cgcggcctgc tctacatgaa
     1681 gggccgtccg acgctggaaa ccttctggca ggggccgtcg gtgggctggg actggggcgg
     1741 caacgccagc cgggccttca ccctgtgcta taatctgcaa tacccggatg ccatcttccg
     1801 ccgcttcccc ggcgtcgagg gcaccgccta tctggtgggc ggcctgggcg tgaactatca
     1861 gaaggccgac gacatcgttc tagccccgat ccgcgccggg gtgggcctgc gtctgggcgc
     1921 caatgtcggc tatctgggct acagccgcaa acgccggatc atgccgttct gaggttgatg
     1981 ggcgccggcc tcagtgcggc ttgaccttgc tgttgagcca gtccatcgcc gccagcaaga
     2041 cgcctgaaac cacgaacgac aggtgcaccg tgacaagcca gaacaggcgc ggttcgtcga
     2101 gcggaacgcc cggttcggtc agcttgagaa acgccttgag cagggcgatc gcggaaatcg
     2161 ccacgatcga ggcgatcagc ttcatcttca ggccagaaaa gtcggcggtc ctcgtggctg
     2221 gcggtgtcga tcttggagac gaagttctcg tagccggaca gaatgacgat caccagcagg
     2281 ttggcggcca gagacaggtc gatcagggtc agggccatga ggatggcgtc ctcgggcgtc
     2341 atcgacagca ggccgcccag ttcgtgaaac agttcctgaa agaacaccac caggagcgcg
     2401 gccagggcga ccaccaggcc gacatagaac ggcgccatca accagcgcga ggcaaacagg
     2461 ccgcgttcca gcagcgtctc gaggcggggc tttatcatcg gtcggcgaat ccctgagcgt
     2521 cagccctgat attccaccac atagcgcacg gtctcgcgac cgttgggcga cagggtgaaa
     2581 cgccaggctg cggcgccgtc gcggatgtcg tggcgccttg gctcgctaac aatcctgnnn
     2641 nnnnnnnggt ggcgcagctc gatcgtgatc gacccggatt tggcgttggc gaggtcgatc
     2701 tcgtagctcc gccggacgcg tccccggctg gctctggtct ccctgaccag gcgcggcctg
     2761 acagagacgt ccatggcccg gccgatggcc aggtcgaagg gctgccccac cggaacatcg
     2821 cgcatggtct gttcgccggc aaaggcagga cgaccgctgg ccgtctccat cacggcaagg
     2881 gcgcccgagg gcagggcctt acccagccct tggttggcct tgttctcaag cctcagcagc
     2941 acggtggcgg cgggaaggtt gtcgtcgcca tagtcgctcc agggatcgac ggtgaccatg
     3001 tagagccgct gaaacgccac gcccttttgg tcgaggaagg cgacctgctt ggtctgacgg
     3061 gcagccaggg taacgggctc aggcagggtg tagagcttgt agtcgcccaa ctctgactgt
     3121 tcggccatct tggcgcgctg ggcggtgacg gtgatctcct cgcgcgcggc gccggccggg
     3181 gccatggaca tcatcaccgg aggtataggc aggggcggcg gtggcggagg cggcggtcgc
     3241 ccatgggtgg tggtgtccat cggccagcag gcgcgcgtga tggcctcggt gtcggccctg
     3301 ggaatgttga cgggcacgcg cgacagcttg ccggccacgg cctgggtcgg ggcgtcgacg
     3361 aagcgtgtcc cgctgctgtt gaccaaggta agccagccgg tcaggtccag ggttttgccg
     3421 tccggcgcga tgcgggcgac gtagtcggcc tgccagttca cgccaatggc gaggtacgac
     3481 agggtcagct tggcggtcgt ggcgaccggc acgtcgatca gggtcgacag cgagggcttg
     3541 tctgac
//
LOCUS       DAOMEO010000207         3545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3003_length_3545_cov_2.820917, whole genome shotgun sequence.
ACCESSION   DAOMEO010000207 DAOMEO010000000
VERSION     DAOMEO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3003_length_3545_cov_2.820917"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="PLH31_06000"
     CDS             complement(<1..84)
                     /locus_tag="PLH31_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88811.1"
                     /translation="MSGLNGGGLGLVGDIGGTNARFALVEFD"
     gene            complement(98..1909)
                     /gene="edd"
                     /locus_tag="PLH31_06005"
     CDS             complement(98..1909)
                     /gene="edd"
                     /locus_tag="PLH31_06005"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HQR88812.1"
                     /translation="MSLNPVIARVTARIIERSRDSRAAYLANMDAAIASNPGRAKLSC
                     ANWAHAFAASPGIDKVRALDPNAPNIGIVSAYNDMLSAHQPLEDYPALIKAAARDVGA
                     TAQFAGGVPAMCDGVTQGRPGMELSLFSRDVIAMATGIALTHDAFDSALYLGVCDKIV
                     PGLVIGALTFSHLPALFVPAGPMTSGLPNSEKARIRALYAEGKVGRAELLEAESASYH
                     GPGTCTFYGTANTNQMLMELMGFHLPGSAFVHPNTPLREALVKEAARRAAAVTAKGNE
                     YIPVGRMIDEKSVVNGVVGLMATGGSTNLALHLVAMAHAAGVILTLEDLDEISRATPL
                     LARVYPNGSADVNHFQAAGGMAFVIRELLKAGLAHEDVLTIAGPGLSRYTQEPVLVDG
                     ELSWRDGAAESLDTAIVRPVSDAFSPEGGLRMLGGNIGRGVMKISAVKPEHHAITAPA
                     AVFQEQEDFIAAFKRGELDRDVVVVMRFQGPAANGMPELHNLSPSISVLLDRGFKVAL
                     VTDGRMSGASGKTPAAIHVTPEAAKGGLLAYVQDGDVITVDATNGDLKVLVDEAVLRA
                     RTPANVPASKPGFGRELFGWMRAGVGPADHGASVLFA"
     gene            complement(1914..2606)
                     /gene="pgl"
                     /locus_tag="PLH31_06010"
     CDS             complement(1914..2606)
                     /gene="pgl"
                     /locus_tag="PLH31_06010"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQR88813.1"
                     /translation="MSQPQIEAFKSRDDLYDAAASALIEALTSAVAKRGQAGFAATGG
                     TTPAPVYDRLALMTAPWDKVVVTLSDDRFVPPSHAGSNEGLVRRHLLVGEAAKARFAP
                     LYFEGVSQEDSALKAEEGVAAASPFGAVLLGVGADGHFASLFPGSPVLAQGLDPETER
                     LVLAAPAGHPAPDIPRLSLTFAALTRTDLIVLLVTGEAKRALLEGPIDPALPVASILK
                     QDRARVRILWAE"
     gene            complement(2609..>3545)
                     /locus_tag="PLH31_06015"
     CDS             complement(2609..>3545)
                     /locus_tag="PLH31_06015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQR88814.1"
                     /translation="HYLGKETVQNLTALRFANVLFEPLWDRNTIDHVQITIAETEKVG
                     DRWPYYDEYGALRDMVQNHMLQLLCLVAMEAPSGFDPDAVRDEKVKVLRSLRPFSKDT
                     VAHDTVRGQYVAGVVEGGAREGYLEEVGKASKTETFVAMKVAIDNWRWDGVPFFLRTG
                     KNLPDRRTQIVVQFKPLPHNIFGQATDGDLFSNRLVIDLQPDENIVLTLMNKRPGLSE
                     EGMRLQSLPLSLSFSQSGGRRRIAYEKLFLDVFRGDRTLFVRRDEVEQAWKFIDGVSA
                     AWAAANIEPAPYAAGTWGPQSSSALISPGGRSWKA"
BASE COUNT          597 a         1157 c         1219 g          572 t
ORIGIN      
        1 gtcgaattcc accagggcga aacgcgcatt ggttccgccg atgtcgccga ccaggccaag
       61 cccgccgcca ttcaagccgc tcatcaagac tgcttcctca agcaaacagc acggaggcgc
      121 cgtggtcggc ggggccgact cctgcgcgca tccagccgaa caattcccgc ccaaagcccg
      181 gcttggatgc cgggacgttt gccggagtgc gagcgcgaag aaccgcctcg tccaccagca
      241 ctttcaaatc gccattcgtg gcgtcgaccg tgatcacgtc accgtcctgc acgtaagcca
      301 gaaggccacc tttggccgct tccggcgtca catggatcgc cgctggtgtc ttgcccgaag
      361 cgcccgacat ccggccgtcc gtgacgaggg ccaccttgaa gccgcggtcc agcaacaccg
      421 agattgacgg cgacaggttg tgcagttcgg gcatgccgtt ggcggccggc ccctggaagc
      481 gcatcaccac cacgacgtcg cggtcgagtt caccgcgctt gaaggcggcg atgaagtctt
      541 cctgttcctg gaacaccgcc gccggggcgg tgatcgcatg gtgctcgggt ttcaccgccg
      601 agatcttcat cacgccgcgc ccgatattgc cgcccagcat gcgcaggccg ccctcggggc
      661 tgaaggcgtc ggagaccggg cggacgatgg cggtatcaag cgactccgcc gcgccgtcgc
      721 gccagctcag ctcgccatcc accaggaccg gttcctgggt gtagcgcgac aggccagggc
      781 cagcgatggt caggacgtct tcatgggcca ggccggcctt cagcagctcg cggatcacga
      841 aggccatgcc gcccgcggcc tggaagtgat tcacgtcggc cgagccattg ggatagacgc
      901 gggccagcag cggcgtggcc ctggaaatct cgtccaggtc ctccagcgtc aggatcacgc
      961 cagcggcatg ggccatggcg accaggtgca gggccagatt ggtcgagccg ccggtggcca
     1021 tcaggccgac cacgccgttg acgaccgact tctcgtcgat catccggccg accgggatgt
     1081 attcgttgcc cttggcagtg acggcggcgg cgcggcgcgc ggcttccttg accagggcct
     1141 cgcgcagcgg cgtgttggga tgcacgaagg ccgagccggg cagatggaag cccatcagtt
     1201 ccatcagcat ctggttggtg ttggctgtgc catagaaggt gcaggtcccg gggccgtgat
     1261 agctggcgct ttcggcctcc agcagttcgg cgcggccgac cttgccctcg gcatagaggg
     1321 cgcggatgcg ggccttttcg gaattgggca ggccggaggt catgggacca gcgggtacga
     1381 acagggccgg caggtggctg aaggtcaggg cgccgatgac taagcccggt acgatcttgt
     1441 cgcagacgcc cagatacagc gcactgtcga aggcgtcgtg ggtcagggcg atgcccgtgg
     1501 ccatggcgat gacgtcgcgc gagaacagcg acagctccat gcctggccgc ccctgcgtga
     1561 cgccgtcgca catggccgga accccgccgg cgaactgggc ggtggcgccg acgtcgcgcg
     1621 ctgcggcctt gatcagggcc ggatagtctt ccagcggctg gtgcgccgac agcatgtcgt
     1681 tataggctga aacgatgccg atattgggcg cgttgggatc cagcgcccgc accttgtcga
     1741 tacccggcga ggcggcgaaa gcgtgggccc agttggcgca ggacagcttg gcgcgccccg
     1801 gattgctggc gatcgccgca tccatgttgg ccagataggc ggcgcggctg tcacggctgc
     1861 gctcgatgat ccgggcggtg accctggcga tgacggggtt caggctcatg cgactactcc
     1921 gcccacagga tgcggaccct ggcgcggtcc tgtttgagga tcgacgcgac aggcagggcc
     1981 ggatcgatgg ggccttccaa caacgcgcgc ttggcctctc cggtcaccag cagcacgatc
     2041 aaatcggtac gggtcagggc ggcgaaggtc aggctgagac gcggaatgtc cggcgcagga
     2101 tgtcccgccg gggcggccag caccaggcgc tcggtctcgg gatccagacc ctgggccagc
     2161 accggcgagc ccgggaacag cgaagcgaag tggccatcag ccccgacacc cagcagcacg
     2221 gcgccgaacg gcgaggcggc ggcgacgccc tcctccgctt tcagggcgga gtcttcctgg
     2281 ctgacaccct cgaaatagag tggcgcgaac cgggccttgg cggcctcgcc gaccagcagg
     2341 tggcgacgga caaggccttc gttgctgcca gcgtgcgacg gcggcacgaa acggtcgtcg
     2401 ctgagggtaa ccacgacctt gtcccagggc gcggtcatca gcgccaggcg gtcatagacg
     2461 ggggccggcg tggtgccgcc cgtggccgca aacccggcct gtccccgctt ggcgacggcc
     2521 gatgtcaggg cctcgatcag cgccgaagcg gcggcgtcat agaggtcgtc gcgggacttg
     2581 aaggcttcga tttggggctg gctcatgatc aggccttcca cgaccggccg ccgggcgaga
     2641 tcagcgcgct cgatgattgc ggcccccagg tgccggctgc atagggggct ggctcgatat
     2701 tggccgcagc ccaggcggcc gaaacgccgt cgatgaactt ccaggcctgc tcaacctcgt
     2761 cgcgacgcac gaacagggtg cggtcgccgc ggaagacgtc aaggaacagc ttttcgtagg
     2821 cgatccggcg acggccgccg ctctggctga acgacagcga cagcggcagg gactgcaggc
     2881 gcatgccctc ttccgacagg cccgggcgct tgttcatcag ggtcaggacg atgttctcgt
     2941 cgggctgcag gtcgatgacg agacggttgg agaacaggtc gccatccgtc gcctggccga
     3001 agatgttgtg gggcagcggc ttgaactgca ccacgatctg ggtacgacgg tcgggcaggt
     3061 tcttgccggt gcgcaggaag aacggcacgc cgtcccagcg ccagttgtcg atcgccacct
     3121 tcatggccac gaacgtctcg gtcttgctgg ccttgccgac ttcttccaga tagccctcgc
     3181 gcgcgccgcc ctcgaccacg ccggccacgt actggccgcg caccgtgtca tgagcgaccg
     3241 tgtccttgct gaagggccgc aaggaccgca gcaccttgac cttttcatcg cggaccgcgt
     3301 cgggatcaaa acccgacggc gcctccatgg cgacaaggca aagcagctgc agcatatggt
     3361 tctgcaccat gtcgcgcagc gcgccgtact cgtcgtagta gggccagcga tcgccgacct
     3421 tctcggtctc ggcgatggtg atctggacgt ggtcgatggt gttgcgatcc cacaagggct
     3481 cgaacagcac attggcgaag cgcagggctg tcaggttctg gacggtttcc ttgcccagat
     3541 agtgg
//
LOCUS       DAOMEO010000208         3528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3018_length_3528_cov_3.093003, whole genome shotgun sequence.
ACCESSION   DAOMEO010000208 DAOMEO010000000
VERSION     DAOMEO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3018_length_3528_cov_3.093003"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLH31_06020"
     CDS             complement(<1..411)
                     /locus_tag="PLH31_06020"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262077.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HQR88815.1"
                     /translation="MHDRAGQLAHPEDLIDLAALTGAYFERQPDMANPAQRVAFGTSG
                     HRGSSLDGAFNEAHILAITQSIVEYRAAQGITGPIFVGRDTHGLSEPAWRTVVEVLVA
                     NGVETLIDDRDGFTPTPAVSHAILAFNRGKTTGLA"
     gene            complement(462..1337)
                     /locus_tag="PLH31_06025"
     CDS             complement(462..1337)
                     /locus_tag="PLH31_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQR88816.1"
                     /translation="MSNKRFSLALHGGAGSAPGHDYSTEIAHMRGLVGVARDRLAAGA
                     SALDVAVETVVALEASGLYIAGKGASPNAAGAYELDACLMDGATGRAGSIAALQGFES
                     PILAARAVMEKTPHVMLAGEGAMAFARSQGLKEIADPERWFTQAGAFESNHPPDHPAG
                     TVGCVVRDAEGRLAAATSTAGVFGKMPGRIGDSPLIGAGAWADDTVAVSCTGQGEYFI
                     RTAVAVQIAHRMRFGGESLEQAAAAAIAGVAALGGDGGLISIDRDGSVAMPFASDGVK
                     RAALLPDGTIFSAAF"
     gene            1504..2502
                     /locus_tag="PLH31_06030"
     CDS             1504..2502
                     /locus_tag="PLH31_06030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HQR88817.1"
                     /translation="MKAVLSTVVGGPETLELSELPDPVAGPGQVLIDVKACGVNYPDV
                     LIIEDKYQFKPTRPFAPGGEVSGLIAAVGEGVTKFKVGDRVLASTGWGGMAEKLALEE
                     GRCTKIPDNMPFDEAAAFILTYGTSYYALKDRGSLKAGETLLVLGAAGGVGLAAVELG
                     KAAGARVIAAASSQEKVDLAIAHGADSGVVYPRGPFDKDGQKALANLIKEACGPNGWD
                     VAYDAVGGDYSEATIRAAGWNGRFLVIGFPSGIPKIPLNLTLLKSCDIVGVFWGASVA
                     RDPAGHAQNVKELMALYAEGKIKPHVSERFPLAKAGDAIAHLASRKAMGKVVVTND"
     gene            2626..3469
                     /locus_tag="PLH31_06035"
                     /pseudo
     CDS             2626..3469
                     /locus_tag="PLH31_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
BASE COUNT          565 a         1187 c         1192 g          584 t
ORIGIN      
        1 ggccaggccc gtggtctttc cgcgattgaa ggccaggatg gcgtgcgaca ccgccggcgt
       61 gggggtgaag ccgtcgcggt catcgatcag ggtttcgacg ccattggcga ccagcacctc
      121 gaccaccgtg cgccaggcgg gctcggacag gccgtgggtg tcgcggccaa cgaagatcgg
      181 gccggtgatc ccctgggcgg cgcggtattc gacgatcgac tgggtgatcg ccaggatgtg
      241 ggcctcgttg aacgccccgt cgaggctgga gccccggtgg ccggaggtgc cgaaggccac
      301 gcgctgggcg ggattggcca tgtcgggctg gcgctcgaaa taggcgccgg tcagggcggc
      361 cagatcgatc aggtcttcgg ggtgggccaa ctggccagcg cggtcgtgca tgtgagaagc
      421 ctatcgatcc aaggttgcgc gttcaattcg gcgacgagcc tttagaacgc cgcgctgaag
      481 attgttccgt ccggcagcag ggcggcgcgt ttgacgccgt ccgaggcgaa cggcatggcg
      541 acactaccgt cccggtcgat gctgatcagt ccgccgtcgc cgcccagggc cgcgaccccg
      601 gcgatggcgg cggccgcggc ctgctccagg ctctcgccgc cgaacctcat gcggtgggcg
      661 atctgcaccg ccaccgccgt gcggatgaag tattcgccct ggccggtgca ggagacggcg
      721 acggtgtcgt cggcccaggc gccggccccg atcagcgggc tgtcgccgat gcgccccggc
      781 atcttgccga acaccccggc cgtcgaggtg gcggccgcca agcgaccctc ggcgtctcgg
      841 acaacgcagc ccacagttcc ggcggggtgg tcgggcggat gattgctctc gaacgctccc
      901 gcctgggtga accagcgctc cggatcggcg atttccttca ggccctgcga ccgtgcgaag
      961 gccatggcgc cctcaccggc cagcatcacg tgcggggtct tttccatcac cgcccgggcc
     1021 gccaggatcg ggctctcgaa accctgtagc gcggcgatcg agcccgcccg gccggtggct
     1081 ccgtccatca gacaggcgtc cagctcatag gcccccgccg cattgggcga ggcgcccttg
     1141 ccggcgatgt agaggccgct ggcttccagg gcgacgacgg tttcgacggc gacgtccagg
     1201 gcgctggctc cagcggccag acggtcgcgc gccaccccga ccagaccgcg catgtgggcg
     1261 atctcggtgc tgtagtcatg acccggggca gaacccgcgc cgccgtgcag ggcgagacta
     1321 aaacgtttgt ttgacattcc gcttccttgg ccgcgcgccc tgcgttagag catggcagcc
     1381 gaaagtggat gccggtttcg gcgtttgcca tgctctacat attcaaaagg agcctgctta
     1441 gcccgtttca agaaacgggc aggctccgcc ggcctcttag cgaataacga cgggagaaga
     1501 cagatgaagg cggttctcag cacggtggtc ggcggccccg aaaccctgga attgtcggaa
     1561 ctgcccgatc ccgtggccgg tcccggccag gtgctgatcg acgtcaaggc ctgcggggtc
     1621 aactatcccg acgtgctgat catcgaggac aagtaccagt tcaagccaac ccgccccttc
     1681 gccccgggcg gcgaggtctc gggcctgatc gccgccgtcg gcgagggcgt gacgaagttc
     1741 aaggtcggcg accgggtgct ggcctcgacc ggctggggcg gcatggccga gaagctggcc
     1801 ctggaagagg gccgctgcac caagattccc gacaacatgc ccttcgatga agcggcggcg
     1861 tttatcttga cctacggcac ctcctattac gccctgaagg atcgcggcag cctgaaggct
     1921 ggcgagaccc tgctggtgct gggcgcggcc ggcggcgttg gcctggcggc cgttgagctg
     1981 ggcaaggcag caggggcgcg agtcatcgca gcggcctcca gccaggagaa ggtcgatctg
     2041 gccatcgccc acggcgccga cagcggcgtc gtctatccgc gcggtccatt cgacaaggac
     2101 ggccagaagg cgctggccaa tctgatcaag gaagcctgcg gccccaatgg ctgggacgtg
     2161 gcctatgacg cggtcggcgg cgactattcg gaagccacca tccgcgccgc cggctggaac
     2221 ggccgcttcc tggtgatcgg cttcccctcg ggcattccca agattccgct gaacctcacc
     2281 ctgctcaagt cctgcgacat cgtcggcgtg ttctggggcg cctcggtggc tcgcgatccg
     2341 gccggccatg cccagaacgt caaggaactg atggccctct atgccgaggg caagatcaag
     2401 ccgcacgttt ccgagcgctt cccgctggcc aaggccggcg acgccatcgc tcacctcgcc
     2461 agccgcaagg ccatgggcaa ggtggtcgtg accaacgact aaggccgttc ctggccctga
     2521 cggcgagccg ctcgtcgtca gggttaaccc tcgctaagga cgaaatttat cgggatgctt
     2581 acgggccatc cccaagactg aacgcggttc tcacgaaagc cgctgatgtc tcagactgct
     2641 ccatccgttc cggccccgca gggaggcgcc cgaccgttcc gcctcaagcg gcgcgccaat
     2701 gagcgggtca agctgctggg cgagaccatg gatctggtgc gccatgaaga ggtcttccat
     2761 ttcgtcgccg cgcggctgaa ccggggcgtc tcctcgatta tcgccaacca caatctccac
     2821 agcctgtacc tggcccgcaa gagcgccagg gtgcgggact tcttcaccgc tgctggcctg
     2881 ggctcgggtg atcggccgct ccagccggca attccatcgt tgcacctatc tggactggcg
     2941 cgacttcttc tgggccaagg ccagcgctga aaactggcgg gtcttcttcg tgggcggcgc
     3001 ccctggcgtg gccgaggcgg cgagcgagac aatcctggcc gaccatccgg gcgtcgagct
     3061 ggccacccat cacggctatt tcgacgccag ccccggttcg gcggaaaaca cggcggtggt
     3121 cgaggcgatc aacgccttca agcctcacat cctgctggtc ggcatgggca tgccgcgcca
     3181 ggagatctgg gtgctggaaa accacaaggc catcaccagc ccctgcgccc acttcacggt
     3241 cggcggggcc tttgactacg aggccggcgt ccagaaggcg gccccgcgct ggatgggcca
     3301 gatgggcgtc gaatggctgt tccggctgct ggccgatccg cgccggctgt tctcgcgcta
     3361 ttgcgtcgag ccctggttcc tggtcgggcc ggcgatggct gatctacgcc aggccttcct
     3421 gacccggccg gcgacccaga tcgacggcgc gaccgccttt ccaggctagc caaggcctct
     3481 gtcctccgcg agtatgatcg ctgatcaccg atccagcaga gatcgggc
//
LOCUS       DAOMEO010000209         3519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3033_length_3519_cov_2.421478, whole genome shotgun sequence.
ACCESSION   DAOMEO010000209 DAOMEO010000000
VERSION     DAOMEO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3033_length_3519_cov_2.421478"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PLH31_06040"
     CDS             <1..173
                     /locus_tag="PLH31_06040"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HQR88818.1"
                     /translation="RVGLPAKSGVGGGIVAVVPGKAVIAVWSPELDRFGTSVVGTAAL
                     EAFAQLTNCSVL"
     gene            245..>791
                     /locus_tag="PLH31_06045"
     CDS             245..>791
                     /locus_tag="PLH31_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQR88819.1"
                     /translation="MSKRILILLGHPDCRPERFGAALASAYADAATAAGHDVRRIDLA
                     DIETPFLAGQWEFDSPPPPHIQAVQQDILWANHIVLLFPLWLGATPAKLKALLEQVFR
                     DGFGMETSPRGPVARLKGRSVRLIVTMGMPAIAFWLLFGAHGLLAVEKGILGLAGMTP
                     IRKTVIGAVESIGGRGRLKWLA"
     assembly_gap    792..823
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(840..>1216)
                     /locus_tag="PLH31_06050"
     CDS             complement(840..>1216)
                     /locus_tag="PLH31_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQR88820.1"
                     /translation="LDKGRVWHVKKPLDAPAMHTAAQALVGLHDFTTFRDMHCQAKSP
                     VKTLDVARVWREGDEVCLAFEARSFLHRQVRSMTGTLVEVGIGRWTAQDVKDALEAKD
                     RTQCGPVAPADGLYLVGVGYGD"
     assembly_gap    1217..1226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1227..1542)
                     /locus_tag="PLH31_06055"
                     /pseudo
     CDS             complement(1227..1542)
                     /locus_tag="PLH31_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515655.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
     gene            complement(1542..2480)
                     /gene="fmt"
                     /locus_tag="PLH31_06060"
     CDS             complement(1542..2480)
                     /gene="fmt"
                     /locus_tag="PLH31_06060"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQR88821.1"
                     /translation="MRIAFLGTPDFAVACLAELVASGHEIACVYSQPPAPRGRGQDLK
                     PSPVHAFAEGLGLPVRTPVSMKAAEEIEAFKALDLDAAVVVAFGQILVRDVLDAPREG
                     CFNLHASLLPRWRGAAPIQRAIMAGDAVTGVQVMRMSEGLDEGPILMSEQVRIDALET
                     AGSLHDKLAAVGARILPVALGAIERGAARETPQSEEGVTYAKKIKSAEARIDWTRPAA
                     EIDCHIRGLSPFPGAWFEAPSDKGPVRVKALLSRVEDADGTPGTVLDDSLLIACGTGA
                     IRLLKAQREGKGAQDASDFIRGFPLKAGTVLEANGA"
     assembly_gap    2537..2546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2595..2951
                     /locus_tag="PLH31_06065"
     CDS             2595..2951
                     /locus_tag="PLH31_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HQR88822.1"
                     /translation="MRSTLIFAVCAAALSLAACSDQHATEIKEGAKAAATDIKDAAKE
                     ISSDPDVKQAGTALKESAKEAGAELKEAAGEATDAVQSAGEKAGDAAKEAGSDIKAGA
                     KKAGQETKEEVHEATR"
     gene            complement(3016..3516)
                     /gene="def"
                     /locus_tag="PLH31_06070"
     CDS             complement(3016..3516)
                     /gene="def"
                     /locus_tag="PLH31_06070"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQR88823.1"
                     /translation="MRVTLASRDFDDALRALMDDMLETMYDAPGIGLAAVQIGEPIRV
                     IVMDLARDGEEPAPRYYVNPEILSASEDTQGYEEGCLSVPDYYDEVERPSKVTLRYMN
                     YQGETVTEEAEGLFAVCVQHEMDHLEGVLFIDHLSRLRRDRAITKVILPHGGRWPRGR
                     GGASSA"
BASE COUNT          548 a         1197 c         1188 g          534 t
ORIGIN      
        1 atcgcgtcgg cctgccggcc aaaagcggcg tcggcggcgg cattgtcgcc gtggtccccg
       61 gcaaggcggt gatcgcggtg tggtcgccgg agctggaccg gttcggcacc tcggtggtgg
      121 gcacggcggc gctggaggcc ttcgcgcagc tgaccaactg ctcggtgttg tagccgcgcg
      181 gacattgagg cgcatcaagg ctggggagcg ttgagtcctg cagactgtcc gcaggagaca
      241 cgccatgagc aaacgcattc tgatcctgct ggggcatccc gattgccggc ccgaacgctt
      301 tggcgcggcc ctggccagcg cctatgccga cgccgcaacg gctgccggcc acgatgtacg
      361 gcggattgac ctggctgaca tcgaaacccc cttcctggcc ggtcagtggg aattcgacag
      421 ccctccgccg ccccacatcc aggccgtgca gcaggatatt ctgtgggcga accatatcgt
      481 cctgctgttt cccctctggc tgggcgcgac gcccgccaag ctcaaggccc tgctggagca
      541 ggtgttccgc gacggctttg gcatggaaac cagccccagg ggcccggtcg cccgactgaa
      601 gggacggtcg gtgcgcctga tcgtgaccat gggcatgccg gccatagcct tctggctgct
      661 gttcggcgcg catggccttc tggcggtgga aaaggggatt ctgggcctgg cgggcatgac
      721 gccgatccga aagaccgtga tcggcgcggt cgagtcgatc ggcggccggg ggcggttgaa
      781 atggctggcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcggaac cctgggcgtc
      841 tagtcgccgt accccacacc caccagatac agcccatcgg ccggcgcgac cggcccacac
      901 tgggtgcggt ccttggcctc cagcgcgtcc ttcacgtcct gagccgtcca ccgcccgatc
      961 cccacctcga ccagggtccc ggtcatcgac cgcacctgcc gatgcaggaa gctccgcgct
     1021 tcgaacgcca gacagacctc atcgccctcg cgccacaccc gggcgacatc cagcgtcttg
     1081 accggcgact tggcctggca gtgcatgtcg cggaaggtgg tgaagtcgtg caggcccacc
     1141 agagcctggg ccgccgtgtg catggccggc gcgtccagcg gcttcttcac gtgccagacc
     1201 cggcccttgt ccagcgnnnn nnnnnnccga gaaccgggcg tgccagtccc cctccgcgat
     1261 ctcgcaggcc aggatgctga ccgcctcgcg ggtcagatgg gcgttgaggg cgttcatgac
     1321 ggtctgggcc ggccagtcgc gctccagatc cacatgcacg acctgtcctg tcgcgtgcac
     1381 gcccgtgtcg gtgcggccgg ccgccgcgat gcggatctcc tggccgcaga aggccttcac
     1441 cgccgcctcg atcgcgccct gcaccgacgg cagggtcgcc tgggcctgga agccgttata
     1501 gggccggccg tcatactcga cgaggaggcg gtagcggggc atcaggcccc attcgcctca
     1561 agcacggtcc cggccttgag gggaaagccc cggatgaagt cgctggcgtc ctgggcgccc
     1621 ttgccctcgc gctgggcctt cagcaggcgg atcgcgccgg ttccacaagc gatcagcagg
     1681 ctgtcgtcca gcaccgtccc aggcgttccg tcagcgtcct cgacgcgcga cagcagggcc
     1741 ttcacccgga ccgggccctt gtccgaaggc gcctcgaacc acgcgccggg gaagggcgac
     1801 aggccgcgaa tgtggcagtc gatctcggcg gccggacggg tccagtcgat gcgggcctcg
     1861 gccgacttga tctttttggc gtaggtgacg ccctcttcgc tctgcggcgt ctcgcgcgcc
     1921 gcgccacgct cgatggcgcc cagggccacg ggcaggatgc gcgcgcccac ggcggccagc
     1981 ttgtcgtgca ggctgccggc cgtctccagg gcgtcgatac gcacctgttc ggacatcagg
     2041 atcgggccct cgtccagccc ctcgctcatc cgcatcacct gaacgccggt caccgcatcc
     2101 ccggccatga tcgcgcgctg gatcggcgcc gcgccgcgcc agcgcggcag cagggaggcg
     2161 tgcaggttga agcagccctc tcggggggcg tccagcacgt cgcgcaccag gatctggccg
     2221 aaggcgacca ccacggcggc gtcgagatcc agcgccttga aggcctcgat ctcttcggcc
     2281 gccttcatcg agaccggcgt gcggacgggc aggcccaggc cttcggcgaa ggcgtggacg
     2341 ggcgagggct tgaggtcctg gccgcgtccg cgcggggccg gcggctggga atagacacag
     2401 gcgatctcgt ggcccgacgc gaccagttcg gccagacagg cgacggcgaa atcaggggtt
     2461 ccgaggaaag cgatgcgcat gggcgcagtt tagcggtccc ggcctcgcat tgaaagccgc
     2521 tgggtaaagc cgctggnnnn nnnnnngacc gtggagcgcc gttatctggc cgacacttct
     2581 ccaggagcat caccatgcgt tccaccctga tcttcgccgt ttgcgccgcc gccctgtcgc
     2641 tggccgcctg ctccgaccaa cacgccaccg agatcaagga aggcgccaag gccgccgcca
     2701 ccgacatcaa ggatgcggcc aaggaaatct ccagcgatcc cgacgtcaag caagccggca
     2761 cggccctgaa ggaaagcgcc aaggaagccg gcgccgaact gaaggaagcc gccggcgagg
     2821 cgaccgacgc ggttcagtct gccggcgaaa aggccggcga tgcggccaag gaagccggct
     2881 cagacatcaa ggccggcgcc aagaaggccg gtcaggaaac caaggaagag gttcacgagg
     2941 cgacgcggta gggcgcggat agctgctccc cctctggggg agctgtcgcg gagcgactga
     3001 gggggttttc acgacctaag ccgagctggc ccccccccgc cctctgggcc acctcccccc
     3061 gtgggggagg atcaccttgg tgatcgcgcg gtcgcggcgc aggcgtgaca ggtggtcgat
     3121 gaagaggacg ccttccaggt ggtccatttc gtgctggacg cagaccgcga acaggccctc
     3181 ggcctcctcg gtcacggtct cgccctgata gttcatgtag cgcagcgtca cctttgaggg
     3241 gcgctcgacc tcgtcataat agtcggggac cgacaggcag ccctcttcgt agccttgcgt
     3301 gtcttccgag gccgaaagga tctcgggatt gacgtagtag cgcggcgccg gctcctcgcc
     3361 gtcgcgggcc agatccatga cgatcacgcg gatcggctcg ccaatctgca cggcggccag
     3421 accgatgccg ggggcgtcgt acatggtctc cagcatgtcg tccatcaggg cgcgcagggc
     3481 gtcgtcgaaa tcgcggctgg ccagggtgac gcgcaaccg
//
LOCUS       DAOMEO010000210         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3037_length_3516_cov_2.319561, whole genome shotgun sequence.
ACCESSION   DAOMEO010000210 DAOMEO010000000
VERSION     DAOMEO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_3516_cov_2.319561"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..>1838
                     /gene="pepN"
                     /locus_tag="PLH31_06075"
     CDS             168..>1838
                     /gene="pepN"
                     /locus_tag="PLH31_06075"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HQR88824.1"
                     /translation="MPTETPQAIRLADYRPFPFEIEETELLFKLEPEATRVLATLKVR
                     RTGDAGEPLVLNGERLKLISAAIDGRALSAADYRLDAEFLTLADVPDVFVLTTEVEIN
                     PAANKALMGLYMSGGRFCTQCEAEGFRTITYFPDRPDVLSRYTVRIEADEAFSYLLSN
                     GNLWEGGELDAADGKGGRHYAIWNDPFPKPAYLFALVAGELDVLQDSFVTMSGRTIDL
                     RVYVDPGQAARGAYAMDALKRAMKWDEDAFGREYDLDLFMIVAVRDFNFGAMENKGLN
                     IFNSSLLLADPETATDADYERIEAVVAHEYFHNWTGNRITCRDWFQLCLKEGFTVFRD
                     QGFSADQRGAAVQRIKDVKALRARQFAEDAGPLAHPVRPSSYMKIDNFYTATIYEKGA
                     EIIRMLKTLLGADAFRKGSDLYFERHDGQATTVEAFIACFADASGRDLTDFFAWYEQA
                     GTPSVSLVSAYDEDARALEITLSQFTAPTPGQPTKKPLPVPVTIGLLDAEGAPLRESE
                     VVILDDATKTLRWEGVASLPVISALRGFSAPVNLTTDARPVDRYVLFAA"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1849..2787
                     /locus_tag="PLH31_06080"
     CDS             <1849..2787
                     /locus_tag="PLH31_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HQR88825.1"
                     /translation="ADPDLFNRWEAGQTLARELILARAGGAADEVGEERYADALGRAL
                     LDPVSEPAFKALLLALPSEPDLSLAMEPADPAAIHAARNALRNRIAVHLGDLLRRLHG
                     DLQTAGEFSADAEAAGKRALRNGCADLLAADPHAINVERITGHFEAAANMTDAMGALW
                     ALVQVGGEARDKALAAFHARWKDEPLVLDKWFAVQARDPSPDVLDRIVALTQHADFDA
                     TNPNRFRALVSTFANFNPSRFHDPSGAGYRFLAEKILAVDAYNPMTAARLVEALGGWR
                     RYADVLGSQMREALKAIAAHPGLSKNVLELATKALG"
     gene            3009..>3516
                     /locus_tag="PLH31_06085"
     CDS             3009..>3516
                     /locus_tag="PLH31_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQR88826.1"
                     /translation="MRSITARSPSQVYIRVAILAALLLLAVYTAFGVKRLQREAEMAP
                     GGAPLAAEASLVASRIEANLAAQRAGLSAASDILQRDPADTMDAAEIALRASGGEAVA
                     VAVAGPAGVQAVAGRDDGADWKAAARAAAASGRSTWTGTAGETGRLYVAAVAPASTGR
                     NFIIASGDP"
BASE COUNT          574 a         1225 c         1128 g          579 t
ORIGIN      
        1 atttcgaacc gggcgctgca tttggggcgc ccgaaagggg aattgcgccc ggttgagcgc
       61 gctttctttg atccggaaca agtccacaca aaaaatatgg cctttagccc ggaccttatt
      121 acttaagggc tctcgcgcag gccgcccggg ccctctagat tgccctcatg cccacagaaa
      181 caccccaggc catccggctc gccgactatc gccccttccc gttcgagatc gaggagaccg
      241 agctcctttt caagctggag cccgaagcca cgcgggtctt ggccacgctc aaggtcaggc
      301 gaacgggcga tgccggcgag ccgctggtcc tcaacggcga gcggctgaaa ctgatctcgg
      361 cggcgatcga cgggcgcgcc ctgagcgcgg cggattacag gctggacgcc gagttcctga
      421 ccctggccga tgtgcccgac gtgttcgtcc tgaccaccga ggtcgagatc aatccggctg
      481 ccaacaaggc gctgatgggt ctctacatgt caggcggtcg cttctgcacc cagtgcgaag
      541 ccgagggctt ccgcacaatc acctacttcc ccgatcgtcc ggacgtgctg agccgctaca
      601 ccgtgcggat cgaggccgac gaggcgttca gctatctgct gagcaacggc aatctgtggg
      661 aaggcggcga acttgacgcc gccgatggta aggggggccg ccactacgcc atctggaacg
      721 accctttccc caagccggcc tacctcttcg cgctggtcgc gggcgagctg gacgtgctgc
      781 aggacagctt cgtcaccatg agcgggcgga ccatcgacct gagggtctat gtcgatcccg
      841 gccaggccgc gcgcggcgcc tatgccatgg acgccctgaa acgcgccatg aagtgggacg
      901 aggacgcctt cggtcgcgaa tacgacctgg acctgttcat gatcgtggct gtgcgcgatt
      961 tcaatttcgg ggccatggag aacaaggggc tgaacatctt caacagctcc ctgctgctgg
     1021 ccgatcccga gaccgccacc gacgccgact acgagcgcat cgaggcggtc gtggcccacg
     1081 aatacttcca caactggacc ggcaaccgca tcacctgccg cgactggttc cagctgtgcc
     1141 tgaaggaagg ctttacggtc ttccgcgacc agggcttttc cgccgaccag cgcggcgcgg
     1201 ccgtgcagcg gatcaaggac gtgaaagccc tgcgcgcccg ccagttcgca gaagatgcgg
     1261 gcccgctggc ccacccggtg cgaccgtcca gctatatgaa gatcgacaac ttctacaccg
     1321 cgactatcta cgaaaagggc gcggagatca tccgcatgct caagaccctg ctgggcgccg
     1381 acgcgttccg gaagggtagc gacctctatt tcgagcgcca tgacggccag gccaccacgg
     1441 tcgaggcctt catcgcctgc ttcgccgacg cgtcaggccg agacctgacc gatttcttcg
     1501 cctggtacga gcaggctggt acgccgtcgg tgtcgctggt cagcgcgtat gacgaggacg
     1561 ccagggcgct ggagatcacc ctcagccagt tcaccgcgcc cacgcccggc cagccgacca
     1621 agaagcccct acccgtcccg gtgaccatcg gtctgctcga cgccgaggga gccccccttc
     1681 gtgagagcga ggtcgtgatc ctggacgatg caaccaagac cctgcgatgg gagggcgtcg
     1741 cttccctgcc ggtgatctcg gccctgcgcg gcttttccgc tccggtgaac ctgaccactg
     1801 acgcccggcc ggtcgatcgc tacgtgctgt tcgccgccnn nnnnnnnngc cgacccggac
     1861 ctcttcaacc gctgggaagc tggccagacc ctggctcgcg agctgatcct agcccgcgcg
     1921 ggcggcgccg ccgacgaggt tggcgaggag cgctatgccg acgcgctggg tcgcgcctta
     1981 ctggacccgg tttcggagcc agccttcaag gccctgttgc tggccttgcc cagcgagccg
     2041 gacctgtccc tagccatgga gccggccgat cctgcggcca tccacgcagc ccgtaacgcc
     2101 ctgcgcaacc gcatcgccgt gcatctgggc gatctgctgc gtcgcctgca tggcgacctg
     2161 cagacggctg gcgagttctc ggccgacgcc gaggctgccg gcaagcgagc cttgcgcaac
     2221 ggctgcgccg acctgctggc ggccgatccg cacgcgatca atgtcgagcg catcaccggc
     2281 cacttcgaag cggccgccaa catgactgac gccatgggcg cgctctgggc cctggtgcag
     2341 gtcggcggcg aggcccgcga caaggccctg gccgccttcc acgccaggtg gaaggacgag
     2401 ccgctggtgc tggacaaatg gttcgccgtc caggcgcgcg atccgtcgcc tgacgtgctg
     2461 gaccgcatcg tcgccctgac ccagcacgcc gattttgacg cgacaaaccc caaccgtttc
     2521 cgggccctgg tctcgacctt tgccaacttc aacccatcgc ggttccacga tcccagcggc
     2581 gcgggctatc gcttcctggc cgagaagatc ctggccgtgg acgcctacaa cccgatgacg
     2641 gcggcgcgtc tggtcgaagc tctgggcggc tggcggcgct atgccgatgt gttggggtcc
     2701 cagatgcgcg aggctctcaa ggccatcgcc gcccatccgg gcctgtccaa gaacgtgctc
     2761 gagctcgcga ccaaggcgct gggctgacac ttggcgtctc cagtcgccta cggatcaatt
     2821 gcgaccaata ggctcatctc ccgtcaaatc ggccgttcgg ccgtgacggg gaccttgggc
     2881 gtgcgttaga ttcaccctgt gtggcgaagt gactcgccag ggatgacgcg gggagctgcg
     2941 ggcgcttgag aattggggat gcaagacccg gaagaccggc ggaagccgga ccttccgcgg
     3001 caggcgtcgt gcggtcgatc accgcgcgat cgccgtcgca ggtctatatc cgcgtcgcca
     3061 ttctcgccgc cctgcttctg ctggcggtct atacagcctt cggggtcaag cgcctgcaac
     3121 gcgaggcgga aatggccccg gggggcgcgc cgctcgccgc cgaggccagc ctggtggcca
     3181 gtcgtatcga agccaatctg gccgcccagc gcgccggcct gtcggcggct tccgatatcc
     3241 tccagcgcga cccggccgac accatggacg ccgccgagat cgccctgcgc gccagtggcg
     3301 gcgaagccgt ggccgtcgcc gtcgccggcc ccgctggtgt ccaggccgtc gccgggcgcg
     3361 atgacggcgc cgactggaag gccgccgcgc gcgccgccgc cgcttcgggt cgctcgacct
     3421 ggaccggtac agccggggaa accggccgcc tctatgtggc cgctgtcgcc ccggcctcga
     3481 ccggtcgcaa tttcatcatc gccagtgggg atccgg
//
LOCUS       DAOMEO010000211         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3044_length_3508_cov_3.116710, whole genome shotgun sequence.
ACCESSION   DAOMEO010000211 DAOMEO010000000
VERSION     DAOMEO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_3508_cov_3.116710"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLH31_06090"
     CDS             <1..527
                     /locus_tag="PLH31_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR88827.1"
                     /translation="LMAARGGGAIVNTASIAGLQAGFGPLAYSTAKCAVIHMSRCAAA
                     ELSPDRIRVNAICPGLIATSIFGASMGLPREVADQMAARVAEVGPKIQPIPKAGLPED
                     IARAALYLASDDSEFVTGTHIVVDGGITVGGRSSWDKSAPSPILSAMGITPEQAEQMR
                     AAMAPQGNQGGGAG"
     gene            533..1438
                     /locus_tag="PLH31_06095"
     CDS             533..1438
                     /locus_tag="PLH31_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR88828.1"
                     /translation="MVLTPNLRGALWMLASAVGFTAMTTLIKYLGPGYPAALQTFYRQ
                     LAGVLILLPLMARDWRGTFHTTRPGILIFRSSAGVLALILSFYAYQELPLADANALSF
                     TRTLWLVPFAGLILREPIGPLRISAAVIGFLGVLIMLQPGADGSGGWVGWPQAAALAA
                     ALLFALTITGMKVMTRDHSPFTLLVYAAVLGLAFSIPPALFVWRWPSWPDLGLLAAMG
                     VIGTLTQGAYIKGMQLGEAAVMAPIDYTRLVFAVLVGFLLFHEVPKTVTLIGAVIVVG
                     STLFISWREHQVSKRAATVKVQTPE"
     gene            complement(1423..2385)
                     /locus_tag="PLH31_06100"
     CDS             complement(1423..2385)
                     /locus_tag="PLH31_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQR88829.1"
                     /translation="MIVPPVIVTGAAGFIGMHVAERLLDRGVDNFNAYYDPALKDARA
                     ARLHSRPGFTMIRMDIAEHEALLELVKSSGAKRIVHLAAQAGVRYSIDNPFAYERSNL
                     AGHMAVLEACRHAGVEHLVYASSSSVYGDRPLNGSGFKETDPAETPVSLYAATKRSCE
                     LISQSYAKLYGFPQSGLRFFTVYGPWGRPDMAYYGFTQKILAGEPIEVYGEGKMARDF
                     TYIDDIVDGVVGVLDHPPARGGHEIYNIGDNDPVGLMEMISTLEATLGAQATKIMRSM
                     QPGDVTATFANIDKLHALTGYKPKVRLAEGLRRFVEWQRAYSGV"
     gene            complement(2401..>3508)
                     /locus_tag="PLH31_06105"
     CDS             complement(2401..>3508)
                     /locus_tag="PLH31_06105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQR88830.1"
                     /translation="LEGADAIKNADAVFIAVGTPTRRGDGHADLSYVYAAAEEIAGLI
                     DGFTVIVTKSTVPVGTGDEVEAIIKRVRPDAQFAVVSNPEFLREGAAIEDFKRPDRVV
                     VGTEDERAQAVMRELYRPLSLNETPIVFTGRRTSELIKYAANAFLAMKITFINEMADL
                     CEKVGADVQQVAKGIGLDKRIGGKFLNAGPGYGGSCFPKDTIALVRTAQQYGAPVKLI
                     ETTVAVNDARKKAMATKVADAIGLSDLTGKTIGVLGVTFKPNTDDMRDAPSLDILPAL
                     QAMGATVQAYDPEGAKEAAHMLPGVDFKAGAYEAAEGADALVILTEWDQFRALDLDRL
                     KLLMNSPVVVDLRNVYKPGEMVRHGFTYASIGRG"
BASE COUNT          560 a         1139 c         1173 g          636 t
ORIGIN      
        1 cgctaatggc tgcgcgcggc ggcggggcaa tcgtcaacac cgcctcgatc gccggcctgc
       61 aggcaggctt tggccccctg gcctattcga ccgccaagtg cgccgtgatc catatgagcc
      121 gctgcgcggc ggccgagctc tcgcccgacc ggatccgggt caacgccatc tgccccggcc
      181 tgatcgccac ctcgatcttc ggcgcctcga tgggtttgcc gcgcgaggtc gccgaccaga
      241 tggccgcccg cgtcgccgag gtgggtccca agatccagcc gatccctaag gccggcctgc
      301 ccgaggacat cgcccgcgcc gcgctgtacc tggccagtga cgactccgaa ttcgtgaccg
      361 gcacccatat cgtggtcgac ggcggcatta cggtgggcgg ccgcagcagc tgggacaaga
      421 gcgcgccgtc gccgatcctt tcggccatgg gcatcacccc cgaacaggcc gagcagatgc
      481 gggctgccat ggccccccaa ggaaaccagg gcggcggggc cgggtgacgg ccatcgtcct
      541 aacgcccaac ctgcgtggcg cgctgtggat gctagcctcg gcggtcggct tcacggcgat
      601 gaccacgctg atcaagtatc tgggcccggg ctatccggcc gcccttcaga ccttctatcg
      661 ccaactggcg ggcgtgctga ttctgttgcc gctgatggcc cgcgactggc gcgggacgtt
      721 tcacaccacc cggccgggca tcctgatctt ccggtccagc gccggggtcc tggccctgat
      781 cctcagcttt tacgcctatc aggaactgcc gctggccgac gccaatgcgc tgtcgttcac
      841 acggacccta tggctggtgc cgttcgccgg cctgatcctg cgcgagccga tcggaccctt
      901 gcggatcagc gcggcggtga tcggatttct gggggtgctg atcatgctgc aaccaggggc
      961 tgacggctcc gggggctggg taggctggcc ccaggccgcg gctctggcgg cggccctgct
     1021 ctttgccctg accatcaccg ggatgaaggt gatgacccgc gaccacagcc ccttcaccct
     1081 gctggtctat gcggcggtgc tgggcctggc gttctcgatc ccgccggctc tgttcgtctg
     1141 gcgctggccc agctggcccg atcttggcct gctggcggcg atgggcgtga tcggcaccct
     1201 gacgcaaggc gcctatatca aggggatgca actgggtgag gcggcggtga tggcgccgat
     1261 cgactatacg cggctggtct tcgcggtgct ggtcggtttc ctgctgttcc acgaagtccc
     1321 gaagacggtg accctgatcg gggcggttat cgtggtgggc tcgaccctgt tcatctcatg
     1381 gcgcgagcat caggtgtcga agcgcgcggc gaccgtgaaa gttcagacgc cagaataggc
     1441 ccgctgccat tcgacgaacc ttcgaagccc ctcggctagc ctgaccttgg gcttgtaacc
     1501 ggtgagggcg tgaagcttgt cgatattggc gaaggtggcg gtgacatcgc cgggctgcat
     1561 ggaccgcatg atcttggtag cctgggcgcc gagcgtggct tcaagggtcg agatcatctc
     1621 catcagccca accgggtcgt tgtcgccgat attgtagatt tcgtgcccgc cacgggcagg
     1681 agggtgatcc agaaccccga cgaccccgtc gacgatgtca tcgatgtagg tgaagtcgcg
     1741 ggccatcttg ccctcgccgt agacctcgat gggctcgccc gcgaggatct tctgggtgaa
     1801 gccgtaatag gccatatcgg gccggcccca ggggccatag acggtgaaga agcgcaggcc
     1861 agactgcgga aagccgtaga gcttggcgta gctctggctg atcagctcgc aggagcgctt
     1921 ggtggcggca tagagcgata ccggcgtctc ggccgggtcg gtttccttga agcctgagcc
     1981 gttcagcggc cggtcgccat agacgctgct ggacgaggca tagaccagat gctcgacgcc
     2041 agcatggcgg caggcctcca gcaccgccat atggccggcc agattggagc gctcataggc
     2101 gaacggattg tcgatcgagt agcggacccc ggcctgggcg gccaaatgca cgatacgctt
     2161 ggcgcccgac gacttcacca gctccagcag ggcctcgtgc tcggcgatgt ccatccggat
     2221 catcgtgaag ccaggccggc tgtgcagccg ggcagcccgg gcgtctttca gggccgggtc
     2281 gtaataggcg ttgaaattgt cgacgccgcg atcaagcagg cgctcggcca catgcatgcc
     2341 gatgaaaccg gccgcgcccg ttacaatgac aggagggacg atgatcggcg tcggcatggt
     2401 tcaaccacgc ccgatcgagg cgtaggtgaa gccatggcgg accatctcgc cgggcttgta
     2461 gacattgcgc agatcgacca cgaccgggct gttcatcaac agcttcaggc gatcgaggtc
     2521 cagagcccgg aactggtccc attcggtcag gatcaccagg gcgtcggcgc cctcggcggc
     2581 ctcatacgcg ccggccttga agtcgacgcc cggcagcatg tgagccgctt ccttggcgcc
     2641 ttcggggtca taggcctgca ccgtcgcgcc catcgcctgc agggccggca ggatgtcgag
     2701 gctgggggcg tcgcgcatat cgtcggtgtt gggcttgaag gtcacgccca gaacaccaat
     2761 ggtcttgccg gtcagatcgc tcaaaccgat ggcgtcggcg accttggtcg ccatggcctt
     2821 cttgcgggcg tcgttcacgg ccaccgtggt ctcgatcagc ttgaccggcg cgccatactg
     2881 ctgggccgtg cggaccaggg cgatggtgtc cttcgggaaa cacgatccgc catagccggg
     2941 accggcattg aggaacttgc cgccgatccg cttgtccagg ccgatgccct tggccacctg
     3001 ctgcacgtcg gccccgacct tttcgcagag gtcggccatc tcgttgatga aggtgatctt
     3061 catcgccagg aaggcgttgg ccgcgtactt gatcagctcg ctggtccgcc ggccggtgaa
     3121 gacgatgggc gtctcgttta gcgacagcgg gcgataaagc tcgcgcatca ccgcctgggc
     3181 gcgctcgtcc tcggtgccaa ccaccacgcg gtcaggacgc ttgaagtctt cgatcgccgc
     3241 gccttcgcga aggaactcgg ggttggagac taccgcgaac tgggcgtcgg ggcgcacgcg
     3301 cttgatgatc gcctcaacct catcgccagt gcccaccggt acggtcgact tggtgacgat
     3361 caccgtaaag ccgtcgatca ggccggcgat ctcttcggcg gcggcgtaga cataggacag
     3421 gtctgcatgg ccgtcgccgc gacgggtggg cgtacccacg gcgatgaaca ccgcgtcggc
     3481 gttcttgata gcgtccgcac cttcgagg
//
LOCUS       DAOMEO010000212         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3048_length_3502_cov_2.569191, whole genome shotgun sequence.
ACCESSION   DAOMEO010000212 DAOMEO010000000
VERSION     DAOMEO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_3502_cov_2.569191"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLH31_06110"
     CDS             complement(<1..202)
                     /locus_tag="PLH31_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR88831.1"
                     /translation="MRHERSLLAKAYEAFNAGDPAALGATLHADVQWPDSLEGGVLNG
                     RAAVVDYFARQFDLMQLDARQVT"
     gene            complement(204..566)
                     /locus_tag="PLH31_06115"
     CDS             complement(204..566)
                     /locus_tag="PLH31_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR88832.1"
                     /translation="MNEEVEALLTSLYDAIDRQDVDAVLAFFHRDARIPDSLEEAALV
                     GRDQIRGYYQRQFATIRVGSSLLATKLLPDGRVEASLHVQVRGAEGGFWWEGPITATY
                     RIENGLITEMTVTEPPGS"
     gene            complement(853..1350)
                     /locus_tag="PLH31_06120"
     CDS             complement(853..1350)
                     /locus_tag="PLH31_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HQR88833.1"
                     /translation="MRVFHTAAIAAFCIVLAACSKGEEKSESGSTPVAAVAAAPSDTE
                     KAALLAALPAPYNAGDLANGQRKFALCRSCHTIAEGGPALTGPNLYGVFGRPSGAVAD
                     YAYSPALKAAGFVWDGEHLDKWLADPRGFMPGTKMAFAGLKDPKDRVDLIAYLKVETG
                     FKPAP"
     gene            1637..2140
                     /locus_tag="PLH31_06125"
     CDS             1637..2140
                     /locus_tag="PLH31_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HQR88834.1"
                     /translation="MDWNEERTAVLKKLWLEGMSASQVARQLGGVSRSAVIGKVHRLG
                     ITVRETPVRQRTSSVRSPSRLQVRARPATAITPTRSAVIRVAPTIVDLEPTSSILGLE
                     THSCRWPIGNPDQNDFGFCGREKPKRGSYCDQHAQGAFRRFQSAGEIEAWTRQGVPEK
                     RIQQRLG"
     gene            2193..2405
                     /locus_tag="PLH31_06130"
     CDS             2193..2405
                     /locus_tag="PLH31_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88835.1"
                     /translation="MNMVLIENTVGSSQVITIIEEFAGHSVSRDLNPGDAARIPVSQF
                     KSITVRETYPDDWLSRGRARNRAAAA"
     gene            2589..3119
                     /locus_tag="PLH31_06135"
     CDS             2589..3119
                     /locus_tag="PLH31_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR88836.1"
                     /translation="MRTHDIGEEPALPLPSYLSYLLYQTEQHRRALAADCIAKHGLTF
                     PKWVALVALRRFGECSMTRLAKLSASDRTTLTRSVDGLIRDGLVERKTSPTDRRIVVV
                     SLTQAGEELLTRIRADIRPLNEEVCSELTAEEQVVMTGYLKKMLGGLIEEPEWKQDVL
                     AFNTPAEALTAPSSSL"
     gene            complement(3116..>3502)
                     /gene="arfB"
                     /locus_tag="PLH31_06140"
     CDS             complement(3116..>3502)
                     /gene="arfB"
                     /locus_tag="PLH31_06140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQR88837.1"
                     /translation="DEIIEKATRASGPGGQHVNKTSTAIELRFDVRNSPTLPEDVKAR
                     LETLGGSRMTLDGVLVLFAQGSRSQEMNRQEARDRLIDLIKRATEKPKPRRPTKPTYS
                     SKLKRLETKGKRAGIKSLRGKPKGDD"
BASE COUNT          634 a         1169 c         1089 g          610 t
ORIGIN      
        1 ccgtcacctg gcgggcgtcc agctgcatca gatcgaactg gcgggcgaaa taatcgacca
       61 cggcagcgcg gccgttcaaa acgccccctt caaggctgtc tggccactgg acatccgcgt
      121 gcagcgtggc gcccaacgcc gccgggtcgc cagcgttaaa ggcttcatag gccttggcca
      181 acagggagcg ttcgtggcgc atctcagctt ccaggaggtt cggtgacggt catttcggtg
      241 atcaggccgt tttcgatacg gtaagtggcc gtgatgggcc cttcccacca gaatccgcct
      301 tccgccccgc gcacctgaac atgcagggag gcctcgacac ggccatcggg gagcagcttt
      361 gtcgcgagca gtgaggatcc aacacggatc gtcgcaaact gacgctgata atagcccctt
      421 atctgatcgc gccccaccag cgcggcctct tccaggctgt cgggaatgcg ggcgtcgcgg
      481 tgaaaaaagg cgagaacggc gtcaacatcc tggcggtcga tcgcgtcgta gagcgaggtc
      541 agaagcgctt ccacctcttc gttcacgggg ctcacctccc gctcgtccgc agcgcgagca
      601 taccgaagat cccgtcaaag tttggaccgg tcagggcggg atcacgcccc agaacacagg
      661 cttgcgcggg gggcgtggcg ttcggaccgg ctcctcgaga cgctgcatat ccatgcgcaa
      721 aatcaggccc tcgcgaagcg tgtaatactg ccgcacctgg ggttctgggc cccaggccgg
      781 cctcgaaggc cgggaggtcg cggcggtcat aggcccggaa gagaggggtg cgcagcgctt
      841 tggcccggct catcacggcg cgggcttaaa gcccgtttcg accttcagat aggcgatcag
      901 atcaacccgg tccttgggat ccttcagacc ggcgaatgcc atcttggtgc cgggcataaa
      961 cccgcggggg tcggccagcc acttgtccag atgctcgcca tcccagacga agccggccgc
     1021 cttcagcgcc ggggaatagg catagtcggc gaccgcgcct gaggggcgcc cgaatacgcc
     1081 atagaggttg ggtccggtca gggcggggcc gccctcagcg atggtgtgac aggaacggca
     1141 gagcgcgaac ttgcgctgac cattggccag gtcgccggca ttataggggg cggggagggc
     1201 agcgagcaaa gcggccttct cggtgtccga tggcgcggcg gcgacggccg caaccggcgt
     1261 cgacccgctc tcagatttct cctcgccctt cgaacaggcg gccaaaacga tgcagaatgc
     1321 cgcgatggcg gcggtgtgaa aaacgcgcat gaaactcccc gagcgacact aagacgcaaa
     1381 ctatgcgacg ccgcgccaca ttcaattact gcccaggttc tgggtcacaa tttcttgtat
     1441 aacatcgtta tgtcgtcaag cttaaggcgg gcctccaaag gcccccctgc gacaattgtt
     1501 ggtacggata tgattccact gacgaaaggt gaattcgcga accgtgggtc tacacataaa
     1561 tatttgacgc ccgttaacca tgatcctacc caaaagaggc gggggggctg ccccgctctg
     1621 ggttggaaaa aagaacatgg actggaatga agagcgcacc gcagttctga agaaactctg
     1681 gctcgaaggc atgagcgcca gccaggtggc ccggcagctg ggcggcgtca gccgcagcgc
     1741 cgtcatcggc aaggttcacc gtctgggcat caccgttcgc gagaccccgg tccgccagcg
     1801 cacctcgtcg gttcgctcgc caagccgcct ccaggtccgc gcccgtccgg ccaccgcgat
     1861 cacgccgacg cgcagtgcgg ttattcgcgt cgccccaacc atcgtagacc tggagccgac
     1921 ctccagcatc ctcggccttg agacccattc ctgccgctgg ccgatcggca atcccgatca
     1981 gaacgacttc ggcttttgcg gccgcgagaa gcccaagcgc ggctcctact gcgaccagca
     2041 cgcccagggc gccttccgcc gtttccagtc ggccggcgag atcgaggcct ggacccgtca
     2101 gggcgtgccg gaaaaacgga tccagcaacg cctcggctga gaccgatctg cttcgccccc
     2161 aacgccaccc tcatctgatt gagtagactt ccatgaacat ggtcctcatc gagaacaccg
     2221 tcggcagcag ccaggtgatc accatcatcg aagagttcgc cggccattct gtgtcgcgcg
     2281 atctcaaccc gggggacgca gcgcgcatcc ctgtcagcca gttcaagtcc atcaccgtcc
     2341 gcgagaccta tcccgatgac tggctaagcc gcggccgggc acgcaatcgg gcggcggccg
     2401 cctgacctct actcccccct gtaaccccga ccctgccttg ccccgcgagc cctctcgcgg
     2461 ggctttttgt gcttgctgag cgcgaccagt gcgattgcgc tcaattatac gtcgcgacgc
     2521 cccgaccgcg attgcgccgg tcaaaacctc atgacatgaa ggcgcccacg cacgcccggg
     2581 gggacgagat gcggacgcac gacatcgggg aagagcccgc cttgccgctg ccaagctatc
     2641 tcagctatct gctctatcag accgagcagc accgccgcgc cctggccgcc gactgtatcg
     2701 ccaagcatgg cctgaccttt cccaagtggg tggccctggt ggctctgcgc cgctttggcg
     2761 agtgctccat gacccggctg gccaagctgt cggccagcga ccgcacgacc ctgacgcgct
     2821 cggtcgacgg cttgatccgc gacggcctgg tcgagcgcaa gacctcgccg accgaccgcc
     2881 gcattgtcgt cgtcagcctg acccaggcgg gcgaagaact gctcaccagg atccgcgccg
     2941 atatccgccc cctcaacgag gaggtctgca gcgagctgac cgcggaagag caggtggtga
     3001 tgacgggcta tctgaagaag atgctgggcg ggctgatcga agagcccgaa tggaagcagg
     3061 acgtgctggc gttcaacacg cccgccgagg cgctgacggc gccgtcttcg tctctctagt
     3121 cgtcgccttt gggcttgccc cgcaggctct tgatccccgc gcgtttgccc ttggtctcca
     3181 gccgcttcag cttgctggaa taggtcggct tggtcggcct tcgcggcttg ggcttttccg
     3241 tcgcgcgctt gatcaggtcg atcagtcggt cgcgcgcctc ctggcggttc atttcctgcg
     3301 agcggctgcc ttgggcaaac agcaccagta cgccgtccag cgtcatgcgg ctgccgccca
     3361 gcgtctccag ccgcgccttg acgtcctcgg gcagggtggg ggagttccgc acatcgaacc
     3421 gaagctcgat ggcggtcgag gtcttgttga cgtgctggcc accgggacct gaggcgcggg
     3481 tcgccttctc gatgatctcg tc
//
LOCUS       DAOMEO010000213         3498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3055_length_3498_cov_1.950624, whole genome shotgun sequence.
ACCESSION   DAOMEO010000213 DAOMEO010000000
VERSION     DAOMEO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_3498_cov_1.950624"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLH31_06145"
     CDS             complement(<1..314)
                     /locus_tag="PLH31_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR88838.1"
                     /translation="MTAAIDRTLAALADPHRRRVVDLLRLRPHRAGELAEAVGLTPPA
                     MSRHLRALRQSGLVEESHPDFDARVRVYHLRPGPMDELKAWLELAETHWVDQLSALKA
                     HV"
     gene            complement(328..>396)
                     /locus_tag="PLH31_06150"
     CDS             complement(328..>396)
                     /locus_tag="PLH31_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88839.1"
                     /translation="NVETVSVEDMERRTGLKITGQV"
     assembly_gap    397..406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<407..747)
                     /locus_tag="PLH31_06155"
     CDS             complement(<407..747)
                     /locus_tag="PLH31_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR88840.1"
                     /translation="MAEGRPDLISAVLYRDPKAALAWLEKAFGFELVFLVEDAEGNLG
                     HSEMRFGAARLMVGGEWSDDHKSPASVGGKNTQTVHIQLSDGDLDAHCERARAAGAEI
                     IVEPATQFYGDR"
     gene            complement(1025..2269)
                     /locus_tag="PLH31_06160"
     CDS             complement(1025..2269)
                     /locus_tag="PLH31_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HQR88841.1"
                     /translation="MKSLLRAVALGAALLLSACASLGQDAAPAQSRLTVLVGIDGFRA
                     DYLDKGDSPTLDALAASGARGAMRPSFPTLTFPNHYTLITGKRPDRNGIVNNVMEDAQ
                     FPGVTFKMSNAQAVRDGRWWDQALPLWVSAETQGYKAGAMFWPGSEAEIAGVRPSRWM
                     VFNQAMPSNDRVDTLLAWLDDPKGPELRLATLYFDVVDTQGHHYGPGSPEARAAVAEV
                     DRAMARLVEGLKARGRFDTTDIVVVADHGMAPVPAAQRWPLDDVVDLSKVRLVTTGAV
                     VGLTPLPGFEAEVEAALLKPLPHLTCWKKGQIPERYHYGRNPRVPAIVCLSETGWYVT
                     TREMMARPGRRDGPIGGQHGFDPYDPAMRAVFVAHGPSIRSGVVLPDFDNVDVYPLLA
                     RISGVKPEDGDGSLKVVGQALK"
     gene            2441..3358
                     /locus_tag="PLH31_06165"
     CDS             2441..3358
                     /locus_tag="PLH31_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR88842.1"
                     /translation="MSIALEATGLTKTYGAVRALDDFSIAIPAGGVFGVLGPNGAGKS
                     TLFRIALGLVRPTSGTARLFGAPAGDVAALRRVGAMIETPRYPPYLTARDTLRMLALE
                     SGFQNPDIAAWLERVSLTHAADRATSGFSVGMKQRLGLAAAFLTRPELVILDEPTSGM
                     DPAGIQEIRGLIVDLARHEGVTVILASHQLDEVKRVCDRVAILSHGRVVAEGGVAELT
                     AGEARLRVTLASPLAPVLAVLADRGQADGPDAVLADIPRAEAPAVIRALVEAGAEIVE
                     ARWREVDLEGVYLKSLGQTPTAAAPEGAA"
     gene            3358..>3498
                     /locus_tag="PLH31_06170"
     CDS             3358..>3498
                     /locus_tag="PLH31_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR88843.1"
                     /translation="MLADAIAAERFRLLRDRSALFWGFGFAPLVGFILAMGGDLFVRT
                     IIK"
BASE COUNT          548 a         1224 c         1149 g          567 t
ORIGIN      
        1 tccacgtgcg ccttcagcgc cgacagctgg tcgacccagt gggtttcggc gagctccagc
       61 caggctttca gctcgtccat cgggcctggc cgcagatgat aaacccgcac ccgggcgtcg
      121 aaatcgggat ggctttcctc gaccaggccc gactggcgca gggcgcgcag gtggcggctc
      181 atggccggcg gggtcaggcc cacggcctcc gcaagctccc ccgcccgatg cggccgcagg
      241 cgcagcaggt cgaccacccg gcgcctgtgc ggatcagcca gggcggccag ggtgcggtcg
      301 attgcggcgg tcacgcgttg cgcccgatca cacctgcccc gtgatcttca ggcccgtgcg
      361 gcgttccatg tcttcgaccg acacggtttc gacattnnnn nnnnnncggt cgccgtagaa
      421 ctgcgtcgca ggctcaacga tgatttcggc gcccgcagcc cgggcgcgct cgcaatgggc
      481 gtccagatcg ccgtcgctca gctggatatg caccgtctgg gtgttcttgc cgcccaccga
      541 ggccgggctc ttgtggtcat cgctccactc accccccacc atcaggcggg ccgcgccaaa
      601 gcgcatttcc gaatggccca gattgccctc ggcgtcctcg accaggaaca ccagctcaaa
      661 gccgaacgct ttttccagcc aggccagggc cgccttcggg tcgcggtaga gaaccgccga
      721 aatcagatcg ggacgtccct cggccatgtc gccactccgt ttcagatatc gtcaatattt
      781 aacgcataat gaaactatct atgcaagggg caagtgtgag ttggcgggct tagcgcctaa
      841 ctaaaaccct gccttcctgg gccttgcgcc caggacccag ggctcagagg tcatggggtt
      901 gggtttgcgt tctaggcccg agcgtatcaa acagccccca agtcagggcc tgccgccgac
      961 atggatcctg ggcgcaaggc tcaggaaggc ggttctttgg gtcatggggc ataggtgtgc
     1021 aagcctattt cagcgcctgc cccaccacct tgaggctgcc atcgccgtct tcgggcttca
     1081 cgcccgagat cctggccagc agcggataga catcgacatt gtcgaaatcc ggcagaacca
     1141 cgcctgaccg gatcgaagga ccatgggcga cgaacaccgc gcgcatggcc gggtcatagg
     1201 gatcaaagcc gtgctggccg ccgatcggcc cgtcccgccg gccgggcctg gccatcatct
     1261 cgcgggtggt cacataccac cccgtctccg aaagacagac aatggccggc acgcgcggat
     1321 tgcggccata gtgatagcgc tcggggatct ggcccttctt ccagcaggtc aggtgcggca
     1381 gcggcttcag cagcgccgct tcgacctccg cttcaaagcc gggcaagggc gtcaggccaa
     1441 cgaccgcgcc cgtggtgacg agccggacct tggacagatc gaccacatcg tccagcggcc
     1501 agcgctgagc ggcgggcacc ggcgccatgc catggtcggc gacgacgacg atgtcggtgg
     1561 tgtcgaaccg cccgcgcgcc ttcaggccct cgaccagtct ggccatcgcg cgatcgacct
     1621 cggcgacggc ggcgcgcgcc tccggcgagc ctggtccata gtggtgaccc tgggtgtcga
     1681 ccacgtcgaa atagagggtg gcaagccgca gctctggtcc cttgggatca tccagccagg
     1741 ccagcagcgt atcgacccgg tcgttcgacg gcatggcctg gttgaagacc atccagcgcg
     1801 agggccgcac cccggcgatt tcggcttccg agcccggcca gaacatcgcc ccagccttat
     1861 agccctgcgt ctcggccgag acccaaagcg gcagggcctg atcccaccag cgaccgtccc
     1921 ggacggcctg cgcattggac atcttgaagg tcacgcccgg aaactgcgcg tcctccatca
     1981 cattgttgac gatgccgttg cggtcgggcc gcttgccggt gatcagggtg tagtggttgg
     2041 gaaaggtcag ggtcgggaac gagggccgca tggcgccgcg cgcgccgctg gcggccaggg
     2101 cgtccagggt cgggctgtcg cccttgtcca gatagtcggc gcggaaaccg tcaatgccca
     2161 ccaggacggt cagccggctc tgggccggcg cggcgtcctg gcccagcgag gcgcaggccg
     2221 acagcagcag ggcggcgccc aaggccacag cgcgcaacag gcttttcatg gcgtaaccct
     2281 ttcgacagtc tggcggcgtc gatgccagtg ggacgtctcg cccgtatgac aggggcgtat
     2341 gacagcggcg taagacctcg tcttaacttg gcgggcgaca agcgcacccc catagttgcc
     2401 gccatcgaca ggattctgcc gttatggaag ccgccctcgc atgagcatcg cccttgaggc
     2461 caccggcctc accaagacct acggggcggt gcgcgcgctc gacgactttt ccatcgccat
     2521 tccggccggc ggcgtgttcg gcgtgctggg tcccaacggc gcgggcaaga gcaccctgtt
     2581 tcgcatcgcc ctgggcctgg tgcggcccac cagcgggacc gcgcggctgt ttggcgcgcc
     2641 cgccggcgac gtggccgccc tgcgccgggt gggcgcgatg atcgagacgc cgcgctatcc
     2701 gccctatctg accgcgcgcg acaccctgcg catgctggcg ctggagagcg ggttccagaa
     2761 ccctgatatc gccgcctggc tcgagcgggt cagtctgacc cacgccgccg accgcgccac
     2821 cagcggtttc tcggtgggca tgaagcaacg cctgggcctg gccgccgcct tcctcacccg
     2881 tcccgagctg gtgatcctgg acgagccgac cagcggcatg gacccggccg gcattcagga
     2941 gattcgcggc ctgatcgtgg acctggcccg acacgagggc gtcaccgtga tcctggccag
     3001 ccaccagctg gacgaggtca agcgcgtctg cgatcgcgtc gccatcctct cgcacggcag
     3061 ggtggtggct gaaggcggcg tggctgaact caccgccggg gaagctcgcc tgcgcgtgac
     3121 cctggcctcc cccctcgccc cggtcctggc ggtgctggct gatcgcggcc aggccgatgg
     3181 acccgacgcc gtgctggccg acattccgcg cgccgaggcg ccggccgtga tccgcgccct
     3241 ggtcgaggcc ggcgccgaga tcgtcgaggc ccgctggcgc gaggtcgacc ttgaaggcgt
     3301 ctatctgaaa tcgctgggcc agacgcccac ggccgccgcc ccggaaggag ccgcctgatg
     3361 ctggccgacg ccatcgccgc cgaacgcttc cgcctgctgc gcgaccgctc ggccctgttc
     3421 tggggcttcg gcttcgcgcc gctggtgggc tttatcctcg ccatgggcgg cgacctgttc
     3481 gtccgcacca tcatcaag
//
LOCUS       DAOMEO010000214         3483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3071_length_3483_cov_2.558926, whole genome shotgun sequence.
ACCESSION   DAOMEO010000214 DAOMEO010000000
VERSION     DAOMEO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3071_length_3483_cov_2.558926"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLH31_06175"
     CDS             <1..840
                     /locus_tag="PLH31_06175"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQR88844.1"
                     /translation="IELTLEDFDVLSRDVPLLVDLQPSGRFLMEDLHYAGGVPAVMKA
                     LEPFLDPTALTVTGAPIGAQYEQAEVWNAEVIRPLDNPVKPDSGIWVLRGNLAPGGAV
                     MKPSAASPELCSHTGKAVVFETIEDFRARIDDPDLDVDETSILVLKGCGPKGYPGMPE
                     VGNMPLPTKLLEKGVKDMVRISDARMSGTAFGTVILHVSPEADAGGPLAIVQNGDVIT
                     LDGSDAELENRLAVWRANQPPPKYTRGYAKLYVDHVLQADKGCDLDFLVGASGSIVTR
                     ESH"
     gene            840..1772
                     /locus_tag="PLH31_06180"
     CDS             840..1772
                     /locus_tag="PLH31_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HQR88845.1"
                     /translation="MDPSIRIFGDWGTSRLRLYLRQGKSVLDRRDGPGVSALSKSRER
                     TFLDLVGDWREAGPSGAILCGMVGSRIGWLEAPYAPCPATADDLRARVLKIEAGGLPV
                     SIIPGLSCVNPLGGPDVMRGEETQILGALELDPALKRGRHLMVLPGTHSKWTVVEDGA
                     ITGFLTAPVGETFALLRQHSTLAAGVGADVQGSARGFAMGLARIVEKGPALLPHLLFE
                     TRARQLLDALPAADAMGFLSGLLIGADAAAARQWFGALGQVTLVGAPALNDFYGQAIA
                     AQGGHTVAIDGDAAVLAGLSALSPPKNSEFHVLA"
     gene            1759..2406
                     /locus_tag="PLH31_06185"
     CDS             1759..2406
                     /locus_tag="PLH31_06185"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HQR88846.1"
                     /translation="MSSPDPVATPSSFGRPPPIVAILRGVKPDEVVAIAKALVDAGIR
                     AIEVPLNSPEPLESIRRLCDAYGEVALCGAGTVLTPQAVDDVAAVGGKLIVTPNTDPD
                     VIARAVELGLTAMPGFATPSEAFAAVKAGARALKLYPASSFGPSHIKAVKDVLPKDIL
                     VYAVGGVGAANLEPWLTAGVAGIGVGGELYRPGYTPQEVGQKAAALIAAWNAQTA"
     gene            2440..>3483
                     /locus_tag="PLH31_06190"
     CDS             2440..>3483
                     /locus_tag="PLH31_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670503.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HQR88847.1"
                     /translation="MQTIDFVILAIYAVAIFGLAQWVSRDKGGHEKTSTDYFLASKAL
                     PWWAIGASLIAANISAEQIIGMSGSGYAIGLAIASYEWMAALTLLIVGKYFLPIFLRN
                     NISTMPQFLEQRYGPSVRTLMAVFWLGLYVFVNLTSILWLGSIAVHTVAGVDQMTALI
                     AIGVFALAYQLWGGLKAVALTDIVQVALLVTGGLIIVFLSLDKIGDGQGVLAGFGRLT
                     AQFPEKFDMILSKDNPHYKDLPGLSVLFGGLWVMNISYWGFNQYIIQRALGAKDLAEA
                     QKGIAFAAYLKLLMPVIVVVPGLAALILAPDLAKPDQAYPEMMKLLPPGLLGLVFAAL
                     VAAIVASLAAKINS"
BASE COUNT          517 a         1245 c         1148 g          573 t
ORIGIN      
        1 attgagctga ccttggaaga cttcgacgtg ctgtcgcgcg acgtgccgct gctggtcgac
       61 ctgcagccct cgggccgctt cctgatggaa gacctgcact atgccggcgg cgtgccggcg
      121 gtgatgaagg cgctggagcc cttcctcgat cccacagccc tgaccgtgac cggcgccccg
      181 atcggcgctc agtacgagca ggcggaggtc tggaacgccg aggtgatccg cccgctcgac
      241 aatccggtca agcccgacag cggcatctgg gtgctgcgcg gcaacctggc ccccggcggc
      301 gcggtgatga agcccagcgc ggccagcccc gaactgtgca gccacaccgg caaggccgtg
      361 gtgttcgaga ccatcgagga cttccgcgcc cgcatcgacg atccggatct ggacgtcgac
      421 gagacctcga tcctggtgct caagggctgc ggccccaagg gctatccggg aatgccggaa
      481 gtcggcaaca tgcccctgcc gaccaagctg ctggaaaagg gcgtcaagga catggtccgc
      541 atcagcgacg cccggatgag cggcacggcc tttggcacgg tgatcctgca tgtctcgccc
      601 gaggccgacg ccggtgggcc gctggccatc gtccagaacg gcgacgtgat cactctggac
      661 ggctccgacg ccgagctgga aaaccgcctg gccgtctggc gcgccaacca gccgccgccg
      721 aaatacaccc gcggctatgc caagctgtat gtcgaccacg tgctgcaggc cgacaagggc
      781 tgtgatctgg acttcctggt cggggcgtcc ggctccatcg tcacccgcga aagccactga
      841 tggacccgtc gatccgcatc tttggcgact ggggcacctc gcggcttcgg ctgtatctgc
      901 gccagggcaa gagcgtgctg gatcgtcgcg acggccccgg cgtcagcgcc ctgagcaagt
      961 cgcgggagcg gaccttcctg gatctggtcg gcgactggcg cgaggcgggc ccctcggggg
     1021 ccatcctgtg cggcatggtc ggctcgcgga tcggctggtt ggaagcgccc tacgccccct
     1081 gcccggccac ggccgacgac ctgcgcgcgc gggtgctgaa gatcgaggcg ggcggcctac
     1141 cggtgtcgat cattcccggc ctgtcctgcg tcaatccgct gggcggccct gacgtgatgc
     1201 gcggcgagga aacccagatc ctgggggccc ttgagcttga tcccgccctc aaacgcggcc
     1261 gtcatctgat ggtcctgccc ggcacccaca gcaagtggac cgtggtcgag gacggcgcga
     1321 tcaccggctt tctcacggcc cccgtcggcg agaccttcgc cctgttgcgc cagcactcga
     1381 ccctggcggc gggtgtcggc gccgacgtgc agggctcggc caggggcttc gccatgggcc
     1441 tggcccgcat tgtcgaaaag ggtccggccc tgctgccgca cctgctgttc gagacccgcg
     1501 cccggcagtt gctggacgcc ctgcccgccg ccgacgccat gggctttctg tcgggcctgc
     1561 tgatcggggc cgacgcggcc gccgcccgcc agtggttcgg cgcgctgggc caggtgaccc
     1621 tggtcggggc gcccgccctc aatgatttct acggccaggc catcgccgcc cagggcggcc
     1681 acaccgtggc catcgacggc gacgccgccg tgctggccgg cctttcggcc ctttcgcctc
     1741 caaagaacag tgagttccat gtcctcgcct gatcccgtcg ccacaccctc gtcttttggc
     1801 cgccccccgc ccatcgtcgc catcctgcgc ggcgtgaagc ccgatgaggt cgtggccatc
     1861 gccaaggccc tggtcgacgc cggcatccgc gccatcgagg tgccgctgaa ttcgcccgag
     1921 ccgctggaga gcatccgccg cctgtgcgac gcctatggcg aggtcgccct gtgcggggcc
     1981 ggcaccgtgc tgacgcccca ggccgtcgat gacgtggcgg cggtcggcgg caagctgatc
     2041 gtcacgccca acactgatcc ggacgtcatc gcccgcgccg tcgaactggg cctgaccgcc
     2101 atgcccggct tcgcgacgcc gtcggaagcc ttcgccgccg tcaaggccgg cgccagggcg
     2161 ctgaagctct atccggccag cagctttggc cccagccaca tcaaggcggt caaggacgtg
     2221 ctgcccaagg acatcctggt ctatgcggtg ggcggcgtcg gcgcggccaa tctggagccg
     2281 tggctgacgg ccggcgtggc cggcatcggc gtcggcggcg aactctatcg tcccggctac
     2341 acgccgcagg aggtcggcca gaaagccgcc gccctgatcg ccgcctggaa cgcccagacg
     2401 gcctgacacc aagaacaacg cgccctcgga gggaaccacg tgcagaccat cgatttcgtc
     2461 atcctggcga tctacgccgt cgccattttc ggcctggccc agtgggtttc ccgcgacaag
     2521 ggcggccacg aaaagacctc caccgactac ttcctggcca gcaaggcgct gccctggtgg
     2581 gccatcggcg cctcgctgat cgccgccaac atctcggccg agcagatcat cggcatgagc
     2641 ggctcgggct acgccatcgg cctggccatc gcctcctacg agtggatggc ggccctgacc
     2701 ctgctgatcg tcggcaagta cttcctgccc atcttcctgc gcaacaacat cagcaccatg
     2761 ccgcagttcc tggagcagcg gtacgggccc agcgtgcgca ccctgatggc ggtgttctgg
     2821 ctgggcctgt acgtgttcgt gaacctgacc tcgatcctgt ggctgggctc gatcgccgtc
     2881 cataccgtgg cgggcgtcga ccagatgacc gccctgatcg ccatcggcgt cttcgccctg
     2941 gcctatcagc tgtggggcgg cctgaaggcg gtggccctga ccgacatcgt ccaggtggcc
     3001 ctgctggtga ccggaggcct gatcatcgtc ttcctgtcgc tggacaagat cggcgacggc
     3061 cagggcgtac tggccggctt tggccgcctc accgcccagt tccccgaaaa gttcgacatg
     3121 atcctgagca aggacaatcc ccactacaag gacctgcccg gcctgtcggt gctgtttggc
     3181 ggcctgtggg tgatgaacat cagctactgg ggcttcaacc agtacatcat ccagcgcgcc
     3241 ctgggcgcca aggacctggc cgaggcccag aagggcatcg ccttcgccgc ctatctgaag
     3301 ctgttgatgc cggtgatcgt cgtggtgccg ggcctggccg ccctgatcct ggcgccggac
     3361 ctggccaagc ccgaccaggc ctatccggag atgatgaagc tgctgccgcc cggcctgctg
     3421 ggcctggtgt tcgccgccct ggtggccgcg atcgtcgcct cgctggcggc caagatcaac
     3481 tcg
//
LOCUS       DAOMEO010000215         3479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3074_length_3479_cov_2.546145, whole genome shotgun sequence.
ACCESSION   DAOMEO010000215 DAOMEO010000000
VERSION     DAOMEO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_3479_cov_2.546145"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLH31_06195"
     CDS             <1..1363
                     /locus_tag="PLH31_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HQR88848.1"
                     /translation="AVDILGAEGRVVAQVQQLGYADVAAEPREVIVDHADKTVRPTFN
                     IAAGDLTRLDGVELVTKGRSNPVWVAHLTPWQSGDVYDPEAIAELERRLRDTAVYDSV
                     SVSIAPRDKVLPDGRRPVIVTLADRPERTIELGAGYSTSEGPGVDARLIRYNRLRRAD
                     TTTYTARLAKLEQRVEGQVALPHWRKPRQTLKMNAAVFRETTDAYDETGATISADLTQ
                     RRMTTSYHTYGVALDLSQSEQLEAARGSAVRETLNLATLTVLGALAWDQSDNILDPTR
                     GWRFEARAEPTTSFGDTTLAYLKAQVQGSAYLPFGEGATTVLAGRVKVGAIFGALVSE
                     IPASRRLFSGGGGSVRGYAYQAIGPRLSDNTPQGGVSLFETSLEVRHRFTERWGGVAF
                     IDAGAIGPERKPDTEDFRFGAGVGVRYDLGFGPIRADIAIPLGKRSGDPAFQIYLSIG
                     QSF"
     gene            1360..>3479
                     /locus_tag="PLH31_06200"
     CDS             1360..>3479
                     /locus_tag="PLH31_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HQR88849.1"
                     /translation="MTDAPEHPIEDAVEEAVETVVEVARKVGGPGWLMIVSLIAVLIV
                     AVFFGGLRYVPITPQGRMFLEARASGLKLGRIGRLKIEGLSGDIWKDFAVRRLTIVDE
                     KGVWLEAHDVRVAWRPTELFGRRFHAERVDAREVVVLRRPTLTPKGKSGAAPVSVDID
                     AFALRLITRPEFSRQAGDFDVNGAFEMARLGGKTGKIDVASRLHRGDRLTLDFDLGRK
                     DTLRLNAVAVEANGGAIAGALGLPVDRSFDLKAKADGAMSKGAFSVTARTGKDVPLDA
                     TGAWDAEGGSARGRIDLGASTLTRRLETMFGPDAAFTVEGRKASGGLYDLTTQIRAEN
                     LVLDSRGLADIGKRRTGPDGLPFTAKIGSLKRVVSIPEMGAGLLKGRVRLDSSGFAVE
                     GDADVSNLSLLGYRLARVTGPAMVRWSKGELTIKAEAAGQGGGGDGVLAVLGANPKAT
                     FEGARQKDGRFLIRSAKVTGPNLVVTGEGDRGILGGLNFKGEARVASLAPIRAGARGG
                     LAAKWSASSANGKPWAFTLDARGQDFALGLAELDRLLGKAPRLSGKGAWNAGVLSIAN
                     ARLEGAAAEVRTSGAMQRDGGLGFKLDWTATGPFRAGPVEVSGKAKGDGAITGTLSAP
                     KADLTADFDAIDLPRLPLTKARLALTFARTPSGADGVARLTGQSAYGPAKAGAAFRFA
                     TGGLDLSDVDIDAGGAKATGSVSLR"
BASE COUNT          525 a         1170 c         1246 g          538 t
ORIGIN      
        1 cgccgtcgat attctcgggg ccgagggtcg ggtggtcgcc caggtccagc aactgggcta
       61 tgccgatgtc gccgccgaac cccgcgaggt cattgtcgat cacgccgaca agacggtgcg
      121 tcccaccttc aacatcgcgg ccggcgacct gacgcgcctg gacggcgtgg agctggtcac
      181 aaaagggcgg agcaacccgg tctgggtggc ccatctgacg ccgtggcagt ctggcgatgt
      241 ctatgatccc gaggctatcg ccgagctgga gcgccggctg cgggacacgg cggtctatga
      301 ctcggtgtcg gtttccatcg cgccgcgcga caaggtcctg ccggacggcc gtcgtcccgt
      361 catcgtcacc ctggcggata ggccggaacg caccatcgag ctgggcgcgg gctattcgac
      421 cagcgagggc ccgggcgtgg atgcgcgcct gatccgctac aaccgtctgc gccgggccga
      481 caccacgacc tatacggcgc gcctggccaa gctggaacag cgcgtcgagg ggcaggtggc
      541 cctgccgcac tggcgcaagc cccggcagac cttgaagatg aatgcggcgg tctttcgcga
      601 aaccacggac gcctatgatg aaaccggggc gacgatcagc gccgacctga cccagcgccg
      661 catgaccacc tcataccata cctacggtgt ggcgctcgac ctgtcgcagt ccgagcaact
      721 ggaggcggcg cgcgggagcg cggtcaggga aaccctcaac ctggcgaccc tgaccgttct
      781 gggcgccctg gcgtgggatc agtcggacaa catcctggat ccaacgcggg gctggcgatt
      841 cgaggcgcgc gccgagccca cgaccagctt tggcgacacc accctggcct atctgaaggc
      901 tcaggttcag ggctcggcct atctgccgtt tggcgaaggc gccaccaccg ttctggcggg
      961 ccgggtaaag gtgggggcga tcttcggggc cctggtctcc gagatccccg cgtcacgccg
     1021 cctgttttcc ggcggcggcg gctcggtgcg gggctacgcc tatcaggcca tcggtccgcg
     1081 gctgtccgac aatacgccgc agggcggcgt ctcgctgttc gaaacctcgt tggaggtccg
     1141 ccatcgcttc accgagcgct ggggcggcgt ggccttcatc gacgccggcg ccatcggccc
     1201 ggagcgcaag cccgacactg aggatttccg cttcggcgcc ggggtcggcg tccgctacga
     1261 cctgggcttt ggcccgatcc gggccgacat cgccatcccg ctgggcaagc gctcgggcga
     1321 cccggccttt cagatctatc tcagcatcgg gcaaagcttt tgaccgacgc gcccgagcat
     1381 cccatcgaag acgcggttga ggaggcggtc gagaccgtcg tcgaggtcgc caggaaggtc
     1441 ggcggacccg gctggctgat gatcgtctcg ctgatcgcgg tgctgatcgt cgcggtgttc
     1501 tttggcggtc tgcgttacgt cccgatcacc ccccagggcc gcatgttcct ggaggcccgg
     1561 gccagcggcc tgaagctggg ccgcatcggg cgactgaaga tcgagggtct ttccggcgac
     1621 atctggaagg attttgctgt tcgccgcctg actatcgtcg acgagaaggg cgtgtggctg
     1681 gaagcccatg atgtccgcgt cgcctggcgg ccgaccgagc tctttggccg gcggttccat
     1741 gccgaacggg tcgacgcccg tgaggttgtc gtgctgcgtc gccccaccct gacgcccaaa
     1801 ggcaagtccg gggccgcgcc ggtgtcggtg gacatcgacg cctttgcgct tcgcctgatc
     1861 acacggcccg agttcagccg gcaggcgggc gacttcgatg tcaacggcgc cttcgagatg
     1921 gccaggctgg gcggaaagac gggcaagatc gatgtggcca gtcggctgca ccggggcgac
     1981 cgcctgaccc tggacttcga tcttggtcgc aaggacaccc tgcgcctgaa cgccgtcgcg
     2041 gttgaggcca atggcggcgc catcgccggc gccctaggcc tgccggtgga ccgctcgttc
     2101 gacctgaagg ccaaggccga cggggcgatg agcaagggcg cgttcagtgt caccgcgcgg
     2161 accggcaagg atgttccgct cgacgcgacc ggcgcctggg acgcagaggg aggctcggcg
     2221 cggggccgca tcgacctggg cgcctccacc ctgacgcgcc gactggagac catgttcggc
     2281 ccggacgccg ccttcaccgt tgagggtcgc aaggcgtcgg gcggactcta cgacctgaca
     2341 acccagatcc gcgccgagaa ccttgttctg gactcccgcg gcctggcaga catcggcaag
     2401 cggcggaccg ggccggacgg cctgccgttc accgcgaaaa tcggttcgct gaagcgggtg
     2461 gtgtcgatcc ccgagatggg ggcggggctg ctcaagggcc gggttcgcct cgattccagt
     2521 ggctttgccg tcgagggtga cgccgatgtc tccaacctgt cgctgctggg ctatcgcctg
     2581 gcgcgggtca ccgggccggc catggtgcga tggtcaaaag gcgagctgac aatcaaggcc
     2641 gaggccgctg gccagggcgg gggaggcgac ggcgtgctgg cggttctggg cgccaacccc
     2701 aaggcgacgt tcgagggggc gcgccagaag gacggccggt tcctgatccg ctcagccaag
     2761 gtcaccggcc ccaaccttgt ggtcaccggc gagggcgacc ggggcattct gggggggctg
     2821 aacttcaagg gcgaggccag ggtcgccagt ctggcgccga tccgcgccgg cgcgcgcggc
     2881 gggctggccg ccaagtggtc ggcgagcagc gccaacggca agccctgggc tttcacactc
     2941 gacgcgcgcg ggcaggactt cgcattgggc ctggccgaac tggaccggct gctgggcaag
     3001 gcgccgcgcc tgtcgggcaa gggcgcctgg aacgcgggcg tgctctccat cgccaacgcc
     3061 aggctggaag gcgcggcggc cgaggtccgc acctccggcg ccatgcagcg cgacggcggc
     3121 ctgggcttca agctggactg gacggccacg gggccgttcc gcgccggacc tgtcgaggtg
     3181 tcgggcaagg ccaagggcga tggcgcgatc acgggaaccc tgtccgcgcc caaggccgac
     3241 ctgaccgctg atttcgacgc catcgacctg cccagactgc ccctgaccaa ggcccgtctg
     3301 gccttgacct tcgcccgcac gccgagcggc gcggacggcg ttgcgcgact gaccggacag
     3361 agcgcctatg ggcccgccaa ggccggcgca gcgttccgct tcgcgacggg gggcctcgat
     3421 ctcagcgacg ttgatatcga cgctggcggc gccaaggcga cgggttcggt ctcgctgcg
//
LOCUS       DAOMEO010000216         3474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3078_length_3474_cov_2.924247, whole genome shotgun sequence.
ACCESSION   DAOMEO010000216 DAOMEO010000000
VERSION     DAOMEO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_3474_cov_2.924247"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLH31_06205"
     CDS             <1..439
                     /locus_tag="PLH31_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR88850.1"
                     /translation="RGGFEAALAERGLKLEPSDIIQGAYTFESGVACGDELLKRTPRP
                     TAIFAGNDEMAAGVLQSARKAGLNVPADLTVVGFDDFQIAQAVWPPLTTIHTPTREIG
                     RMAAEKLIDAEGRERRERREERDPARTEPNLVVRESSGPAPSV"
     gene            551..2005
                     /gene="uxaC"
                     /locus_tag="PLH31_06210"
     CDS             551..2005
                     /gene="uxaC"
                     /locus_tag="PLH31_06210"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQR88851.1"
                     /translation="MARPLIFHEDRLFPADERTRSIARELYTSVKALPIISPHGHTDP
                     SWFATNAPFQDATDLLLAPDHYLFRMLYSQGISLDALKVRSKDGVPGTDPREAWRLLA
                     QNFHLFRGTPSWIWLNHVFSQVFGLEVFFSAETSDLYFDTINEALATDAFRPRALFDR
                     FNIETLATTEGPHETLKHHHAIRDSGWGGHVITAYRPDAVIDFEDERSPRAFERFAEV
                     SGQDVYSWQGYLEAHRQRRLAFIEAGATSSDHGHPTAATADLSDAETEALFLSLVKGE
                     VTPQKAELFRAQMLTEMARMSLDDGLVMQIHPGSHRNHNVGLLASHGRDKGADIPVQT
                     EYVNALKPLLSKFGNDPRLSIILFTLDETSYSRELAPLAGHYPVLKLGPSWWFHDSPE
                     GMMRFREQVTETAGFYNTVGFNDDTRAFLSIPARHDVARRVDSSFLARMVAEHRMDLV
                     EAQELIVDLTYTLPKRAYKLDQPRPTPASAQAAQ"
     gene            2024..2872
                     /gene="kduI"
                     /locus_tag="PLH31_06215"
     CDS             2024..2872
                     /gene="kduI"
                     /locus_tag="PLH31_06215"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQR88852.1"
                     /translation="MFTKTYHATHPDMMFAVSNDDLRERYLMDGLFAENEIVLHYNHA
                     ERFVIGGAAPVSTAVKLPDQTEPASAAGHPFLERRELGAINVGGGKGKVTVDGVAYDI
                     EPRDGIYVTMGSKDVVFESLDAANPARFYLTSLPAHAAFETKKLEFANAVPLERGALE
                     TSNERTIYQYIVPTTCKSAQLLLGMTVLKPGSVWNTMPPHLHDRRSEAYFYFGLGDND
                     RVFHYMGEPDEMRHIVIANEEAVMSPPWSIHMGSGTSNYTFIWAMGGENLDYTDMNVL
                     DICQLK"
     gene            3023..>3474
                     /locus_tag="PLH31_06220"
     CDS             3023..>3474
                     /locus_tag="PLH31_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR88853.1"
                     /translation="MANPFSLEGKVALVTGANTGLGQAVAVALAEAGANIAAAGRSEP
                     TETKAAVEATGRKFLSIKADFGSIEPVQRVIDETVAEFGKVDILVNNAGIIRRADSIE
                     FTEADWDAVMDTNLKVVFFLTQAFARQALKQGPVNGVAGKVINIASLLS"
BASE COUNT          634 a         1196 c         1073 g          571 t
ORIGIN      
        1 gcgagggggc ttcgaggcgg cgctggccga gcgcggcctg aagctggagc ccagcgacat
       61 cattcagggc gcctatacct ttgagtctgg cgtggcatgc ggcgatgagc tgctgaaacg
      121 tacgccgcgt cccaccgcca tcttcgccgg caatgacgaa atggcggccg gcgtgctgca
      181 atcggcccgc aaggccgggc tgaacgtacc ggcggatctg acggtcgtcg gcttcgatga
      241 cttccagatc gcccaggccg tttggccgcc cttgacgacc atccacacgc ccacgcgcga
      301 gatcgggcgg atggcggccg aaaagctgat cgacgcagag ggccgagaac gccgtgagcg
      361 ccgcgaagag cgcgatccgg cgcggaccga acccaatctc gtcgttcggg agtcgtcggg
      421 ccctgcgcct tcggtgtagg ctgacctgac gacgggctgg cttgcatcca gtcatgacac
      481 cggttaccaa gcgcgcgtca gaagcggcag accgcaaagc cgccggctgg agaggaagca
      541 ggagaaatcc atggccaggc ccctgatatt ccacgaagat cgactgttcc ccgccgacga
      601 gcgcacgcgg tcgatcgcgc gcgagctcta tacgagcgtc aaggcgctgc cgattatcag
      661 cccgcacggc cataccgatc ccagctggtt tgcgaccaac gcgccgttcc aggacgccac
      721 cgacctgctg ctggcgccgg accactatct gttccgcatg ctctacagcc agggcatctc
      781 gctggacgcg ctgaaggtgc ggtccaagga tggcgtgcct ggaactgacc ctcgcgaagc
      841 ctggcgtctg ctggcccaga acttccacct gtttcgcggc acgccgtcgt ggatctggct
      901 caaccacgtg ttcagccagg tgttcgggct ggaggtgttc ttctccgccg agacgtcgga
      961 tctctatttc gacaccatca atgaggccct ggcgaccgac gccttccggc cgcgcgccct
     1021 gttcgatcgc ttcaacatcg agaccctggc cactaccgag gggccgcacg agacgctcaa
     1081 gcaccaccac gccatccgcg acagtggctg gggcggtcat gtgatcacgg cctatcgccc
     1141 cgacgcggtc atcgacttcg aggacgagcg tagcccacgc gccttcgagc gcttcgccga
     1201 ggtgtcgggc caggacgtct atagctggca gggctatctg gaagcccacc gccagcgtcg
     1261 cttggctttc atcgaggcgg gcgcgacctc gtcagatcac ggccacccga cggcggcgac
     1321 cgccgatctg agcgacgccg agaccgaggc tctgttcctc agcctggtga agggcgaggt
     1381 gacgccgcag aaggccgagc tgttccgcgc ccagatgctg acggaaatgg ccaggatgag
     1441 cctggacgac gggctggtga tgcagatcca ccctggctcg catcgcaatc acaatgtggg
     1501 cctgctggcc tcgcacggtc gcgacaaggg cgccgacatc ccggtgcaga ccgagtacgt
     1561 caacgccctg aagccgctgc tcagcaagtt cggcaacgac ccgcgcctgt cgatcatcct
     1621 cttcaccctg gacgagacca gctacagccg cgaactggcc ccgctggccg gtcattatcc
     1681 ggtgctgaag ctgggtccgt cctggtggtt ccacgacagc ccggaaggca tgatgcggtt
     1741 ccgcgagcag gtcaccgaga ccgccggctt ctacaacacc gtcggcttca acgacgacac
     1801 ccgcgccttc ctgtcgatcc cggcccgcca cgacgtggcc cgccgcgtcg actcctcgtt
     1861 cctggcccgc atggtcgccg aacaccgcat ggacctcgtc gaggcccagg agctgatcgt
     1921 cgacctgacc tacaccctgc ccaagcgggc ctacaaactc gatcaacccc ggccgacgcc
     1981 tgcgtcggcc caagccgccc agtaagatct ccggagctcc atgatgttca ccaagaccta
     2041 ccacgccacc catccggaca tgatgttcgc ggtcagcaat gacgacctgc gcgagcgcta
     2101 tctgatggac ggcctgttcg ccgagaacga gatcgttctg cactacaacc acgccgaacg
     2161 cttcgtgatc ggcggcgcgg cgccggtttc gaccgccgtg aagctgcctg accagaccga
     2221 gcccgcctcg gccgccggcc accccttcct cgagcgccgc gaactgggcg ccatcaatgt
     2281 cggcggcggc aagggcaagg tcacggtcga cggcgtcgcc tatgacatcg aaccgcgtga
     2341 cggcatctat gtgaccatgg gctccaagga cgtggtgttc gagagcctgg acgccgccaa
     2401 tccggcccgc ttctacctga ccagcctgcc ggcccacgcc gcgttcgaga ccaagaagct
     2461 ggaattcgcc aacgccgttc cgctggagcg cggcgcgctg gagacctcca acgagcgcac
     2521 catctaccag tatatcgtgc cgaccacctg caagtcggct cagctgctgc tgggcatgac
     2581 cgttctgaag ccgggcagcg tctggaacac tatgccgccc catctgcacg accgtcgttc
     2641 ggaagcctat ttctacttcg gtctgggcga taacgaccgg gtcttccact atatgggcga
     2701 gccggacgag atgcgtcata tcgtgatcgc caacgaggaa gccgtcatga gcccgccgtg
     2761 gtcgatccac atgggttcgg gcacctcgaa ctacacgttc atctgggcga tgggcgggga
     2821 gaacctcgac tataccgaca tgaacgtgct ggacatctgc cagctgaagt agggctttcg
     2881 agcccaatcc gctccggctg tcatcccggc cggggcggag cgtagagccg ggaccgcttc
     2941 gagcctcagc gctttttgcg gtcccggatc gacctttcag gtcgtccggg atgacatgct
     3001 ccagcatcag gaaaacagca ccatggccaa tccgttcagc ctcgaaggca aggtcgcgct
     3061 cgtcaccggc gccaacacgg gcctcggtca ggccgtggcc gtggccctgg ccgaagccgg
     3121 cgccaacatc gccgccgccg gtcgcagcga gcccaccgag accaaggccg ccgtcgaggc
     3181 gaccggtcgc aagttcctgt cgatcaaggc cgacttcggc tcgatcgagc ccgttcagcg
     3241 cgtgatcgac gagaccgtgg ccgaatttgg caaggtcgat atcctggtca acaacgccgg
     3301 catcatccgc cgcgccgact ccatcgagtt caccgaggcc gactgggacg cggtgatgga
     3361 caccaacctg aaggttgtct tcttcctgac ccaggccttc gccaggcagg cgctgaagca
     3421 gggtccggtc aatggcgtcg ccggcaaggt gatcaacatc gccagcttgc tgtc
//
LOCUS       DAOMEO010000217         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3084_length_3470_cov_2.602050, whole genome shotgun sequence.
ACCESSION   DAOMEO010000217 DAOMEO010000000
VERSION     DAOMEO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_3470_cov_2.602050"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1454)
                     /locus_tag="PLH31_06225"
     CDS             complement(128..>1454)
                     /locus_tag="PLH31_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HQR88854.1"
                     /translation="CRAAADAILENHLDGEPERTGALDVLAQHVMGCACSEPFDLLAL
                     HDEIRTAGPYADLSWEDFEAVVDFVSTGGYALRTYDKFRRIVQGTDGLWRARNAQTVQ
                     RHRMNVGTIVTAATVNIRVGGGRKPVGGRKIGEAEEGYFEQLTPGDTFVFAGQVWRFN
                     GIVGADAFVSPAPNDDPKMPSWGGSKFPLSTYLASRVRKMMHDEAGWTALPPDVQEWL
                     AWQKVRSALPDEHEMLLETFPRGKRFHMVCYPFDGRIAHNTLAMLLTRRLDRLGVGPL
                     GFVCNDYALNIWSLKPMDDLDLDELFDQDMLGDDLEAWLEESFMMKRTFKACALIERR
                     YPGEEKSGRQVTFSTDLIYDVLRRHQPDHLLLRCARMDAASGLLDVARLGDMLARVKG
                     NIRNIRLDRVSPFAVPLMLEIGRERAPGDAAGEMILAEAEEDLIAEAMA"
     assembly_gap    1455..1464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1465..2563)
                     /locus_tag="PLH31_06230"
     CDS             complement(<1465..2563)
                     /locus_tag="PLH31_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR88855.1"
                     /translation="MSAAAALPPRFQDWFAARGWAARPHQLEMLAKAREGKSALLIAP
                     TGGGKTLAGFLPSLVELSERPPRNTPAGIHTLYISPLKALAVDVERNLMTPIREMGLK
                     VVAESRTGDTGESRKARQRTRPPDILLTTPEQLALFCAWEGAREYFADLSCVIIDEAH
                     AIWPSKRGDLLALHLARLQQFAPNLRRVGLSATVDDPGLVRRWLGSGGEDSVDLVLGS
                     GGAPPVVEVLVSGGRVPWAGHTAEHAMAEVYEIIKGAQTALIFVNTRFQAEFAFQKLW
                     ELNDEGLPIALHHGSLSAEQRRKVESAMTRGELRAVVCTSTLDMGIDWGDVDLVIQLA
                     APKGASRMVQRIGRANHRLDEPSRALFVPASR"
     gene            2700..>3470
                     /locus_tag="PLH31_06235"
     CDS             2700..>3470
                     /locus_tag="PLH31_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR88856.1"
                     /translation="MTSKALVVLLGLVAVVGAMPVQARQTAAPTATVAADDQAFAALL
                     KRGQAGDASAYVDLAFEYLDRDHARYDVTVAEQWLTSGVAAGVPEAMVELGNLYFDGD
                     HVPQDRRKALGLYRRAADLGDLDGLYNVGIVYEGGGGIAANLAEALRWYRRAADAGSE
                     IGMFQVGQAFQHGRGVRVDMRQAVFWLSKAVAAGSSAAMNDLGALYSAGDGTPQDDAE
                     ALKLFIAAARNGSAIAMANVAVVHYNGEGVPVDYVEAIR"
BASE COUNT          540 a         1144 c         1211 g          565 t
ORIGIN      
        1 aagtgcaggt cggcgacgac cagggtccgc tcgacctcca gccacatcgc cccggatccg
       61 cgcagggtca cggtggcgcc gaacagctcg acatggagac cgccacaggc gctggtctcg
      121 aaccgggtca agccatggcc tccgcgatca ggtcttcctc ggcctcggcc aggatcatct
      181 cgccggccgc atcccccggc gcgcgctcgc ggccgatttc cagcatcagc ggcacggcga
      241 agggtgagac acggtccagc ctgatattgc ggatgtttcc cttcacgcgg gccagcatgt
      301 cgcccagccg cgccacgtcc agcaagccgc tggcggcgtc catgcgcgcg cagcgcagca
      361 gcaggtggtc gggctggtgg cggcgcagga cgtcatagat caggtcggtc gagaaggtca
      421 cctgccggcc ggacttctcc tcgccgggat agcgccgctc gatcagggcg caggccttga
      481 aggtgcgctt catcatgaag ctctcctcca gccaggcctc cagatcgtcg cccagcatgt
      541 cctggtcgaa cagctcgtcc agatccagat cgtccatggg cttcagcgac cagatgttca
      601 gggcgtagtc gttgcagaca aagccgagag gcccgacgcc cagccggtcc agccgccggg
      661 tcagcagcat ggccagggtg ttgtgcgcga tgcgtccgtc gaacggataa cagaccatgt
      721 ggaaccgctt cccgcgcggg aaggtctcca gcagcatctc gtgttcgtca ggcagggccg
      781 agcgaacctt ctgccaggct agccattcct gaacgtccgg gggcagggcg gtccacccgg
      841 cctcgtcatg catcatcttg cgcacgcggc tggccagata ggtcgacagt ggaaacttcg
      901 agccgcccca gctgggcatc ttggggtcgt cgttgggggc cggactgacg aaggcgtcgg
      961 cgccgacaat accgttgaac cgccagacct ggccggcgaa gacgaaggtg tcgccgggcg
     1021 tcagctgctc gaaatagcct tcctcggcct cgccgatctt gcgtccgccg accggtttgc
     1081 gcccgccgcc gacccggata ttgaccgtgg ccgccgtgac gatggtgccg acgttcatgc
     1141 gatggcgttg cacggtctgg gcgttgcggg cccgccagag gccgtcagtc ccctgcacga
     1201 tgcggcggaa cttgtcgtag gtcctcaggg catagccgcc ggtcgagaca aaatcgacga
     1261 cggcctcgaa gtcttcccac gacagatcgg catagggacc ggcggtgcgg atctcgtcgt
     1321 gcagggccaa caggtcgaag ggctcggaac aggcgcaccc catgacgtgc tgggccagaa
     1381 cgtccagcgc gccggtgcgt tcgggttcgc cgtcgaggtg gttttccagg atggcgtcgg
     1441 cggcggcgcg gcatnnnnnn nnnnaccggc tggcgggcac gaacagggcg cgactgggct
     1501 cgtccaggcg gtgattggcg cggccgatgc gctggaccat ccgcgaggcg cccttgggcg
     1561 cggccagctg gatgaccaga tcgacgtcgc cccagtcgat gcccatgtcc agcgtcgagg
     1621 tgcagaccac ggcccgcagc tcgccccggg tcatggcgga ctcgaccttg cgccgttgct
     1681 cggccgacag gctgccgtgg tgcagggcga tgggcaggcc ctcgtcgttc agttcccaca
     1741 gcttctggaa ggcgaactcg gcctggaagc gggtgttgac gaagatcagg gccgtctggg
     1801 cgcccttgat gatctcatag acctcggcca tggcgtgttc ggctgtgtgc ccggcccagg
     1861 gcacgcgccc ccccgagacc agcacctcca caacgggcgg cgcgccgccg gagccgagga
     1921 ccaggtcaac gctgtcctcg ccgccgctgc ccagccaacg ccgcaccagc cccggatcat
     1981 cgacggtggc tgacagtcct acccgccgca ggttcggcgc aaactgctgc aggcgcgcca
     2041 gatgcagggc cagcaggtcg ccgcgcttgg acggccagat cgcgtgggcc tcgtcgatga
     2101 tcacgcagga caggtcggcg aagtattccc gcgcgccttc ccacgcgcag aacagggcca
     2161 gttgctcggg cgtggtcagc aggatgtctg gcggccgagt gcgctggcga gccttgcggg
     2221 attcgccggt gtcgcccgtg cggctctcgg cgaccacctt caggcccatc tcgcggatcg
     2281 gggtcatcag gttgcgttcg acatccaccg ccagggcctt gagcggcgag atgtagagcg
     2341 tgtggatgcc ggccggcgta ttgcgcggcg ggcgttcgga tagctcgacc aggctgggca
     2401 ggaagcccgc cagggtcttg ccgccgccgg tgggcgcgat cagcagggcg cttttgccct
     2461 cgcgcgcctt ggccagcatc tcaagctgat gcgggcgagc cgcccagccg cgtgcggcga
     2521 accagtcctg gaagcgggga gggagggcgg cggccgctga cattgcctgt cgctataaca
     2581 tgttcccgtt ccgtttccag accgcaagcg cacggtagcg acgctactcg cctgtgaggc
     2641 agcaacgctt gacgaccgtc gcccggcgcc aacaatcgct ggagttgtcc ggggtggtca
     2701 tgacttcaaa ggcgcttgtg gttctgctgg gactggtcgc ggtggtgggc gcgatgcctg
     2761 tccaggcgcg tcagaccgca gcgccgaccg cgaccgtggc cgctgacgac caagccttcg
     2821 ccgccttgct gaagcgcggc caggccggtg acgcgtcagc ctatgtcgat ctggctttcg
     2881 agtacctgga cagggaccat gcccgctatg acgtgaccgt cgccgagcaa tggctgacga
     2941 gcggcgttgc ggcgggtgtc ccggaagcga tggtcgagct gggcaatctc tatttcgacg
     3001 gcgatcacgt tcctcaggac cgtcgcaagg cgctggggct ctaccgccgc gccgccgacc
     3061 tgggcgacct ggacggcctc tacaatgtcg gcatcgtcta cgaaggcggc ggcgggattg
     3121 cagccaatct ggccgaggcc ctgcgctggt atcgccgggc ggccgacgcg ggttcggaga
     3181 tcgggatgtt ccaggtcggc caagccttcc agcatgggcg cggtgtgcgg gtcgacatgc
     3241 gtcaggcggt gttctggctg agcaaggcgg tcgcggcggg atcatcggcg gccatgaacg
     3301 acctgggcgc gctctattcc gccggcgacg ggacgccgca ggatgacgcc gaggccctga
     3361 agctgttcat cgccgctgcc cgcaacggct cggccatcgc catggcgaat gtcgcggtgg
     3421 tccactacaa cggcgagggc gttccggtcg actatgtgga ggctattcgc
//
LOCUS       DAOMEO010000218         3461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3091_length_3461_cov_2.307105, whole genome shotgun sequence.
ACCESSION   DAOMEO010000218 DAOMEO010000000
VERSION     DAOMEO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_3461_cov_2.307105"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /gene="guaA"
                     /locus_tag="PLH31_06240"
     CDS             complement(<1..745)
                     /gene="guaA"
                     /locus_tag="PLH31_06240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQR88857.1"
                     /translation="MTAPVHHQRVLIVDFGSQVTQLIARRVREAGVYCEIHPFDKAEA
                     IVDDYAPSAIILSGGPASVLEADSPRIGAKLFDLGVPLLAICYGQQLLCDVLGGKVEG
                     GHAGEFGRAELTIGKANPLFEGLAGVGKLETVWMSHGDRVTAIPDGFEVIATSPGAPF
                     AAIADDARKIYAVQFHPEVVHTVNGTQIYRNFLKLAGLRGDWTMAAFRQEMVEKIRAQ
                     VGTGKVICGLSGGVDSSVAAVLIHEAIGDQ"
     gene            complement(925..>1182)
                     /locus_tag="PLH31_06245"
     CDS             complement(925..>1182)
                     /locus_tag="PLH31_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88858.1"
                     /translation="EQDSVLIDAADFVKPGGRLVYVTCSLLVEENEDRVTAFLERRPD
                     FAVKPITSDAIAEHVSAQGYLRLTPHTAGTDGFFAAVLERQ"
     assembly_gap    1183..1192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1193..2186)
                     /locus_tag="PLH31_06250"
                     /pseudo
     CDS             complement(1193..2186)
                     /locus_tag="PLH31_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517064.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
     gene            complement(2191..>2432)
                     /locus_tag="PLH31_06255"
     CDS             complement(2191..>2432)
                     /locus_tag="PLH31_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR88859.1"
                     /translation="RLERASANFRETFPLFAAAVIVTYLSGLIGYLTAHGALIYVAAR
                     ALYMPLYAFGVPVLRSIVWLISFVGIGMVLAAMVV"
     assembly_gap    2433..2442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2535..>3461)
                     /locus_tag="PLH31_06260"
     CDS             complement(2535..>3461)
                     /locus_tag="PLH31_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQR88860.1"
                     /translation="RLIVVDDGFHAVGLITVKDIEKAQAHPLAAKDDKGRLLVGAAST
                     VGDAGFERSMGLVDAGVDVVVIDTAHGHSNQVAQAVSRLKREANRVQIVAGNIATYDA
                     ARALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIMEAARAARESGTPVIADGGI
                     KYSGDLAKAIAAGASTAMMGSMFAGTEEAPGEVFLYQGRSYKSYRGMGSVGAMARGSA
                     DRYFQKEVTDSFKLVPEGIEGQTPFKGAIAPVLHQLVGGLRAAMGYVGAPTIAEFQSR
                     ARFVRITGAGLRESHVHDVMITREAPNYPSAV"
BASE COUNT          563 a         1157 c         1191 g          530 t
ORIGIN      
        1 gctggtcgcc gatggcttcg tggatcagca cggccgcgac cgagctgtcg acgccgcccg
       61 acaggccgca gatcaccttg ccggttccga cctgggcgcg gatcttctcg accatctcct
      121 ggcggaaggc cgccatggtc cagtcgcccc gcaggccggc cagcttcagg aagttgcgat
      181 agatctgggt tccgttgacg gtgtggacca cctcggggtg aaactgcacc gcatagatct
      241 tgcgggcgtc gtcggcgatg gccgcgaacg gcgcgccggg cgaggtggcg atcacctcga
      301 agccatcggg aatggcggtg acgcgatcgc catggctcat ccagacggtt tccagcttgc
      361 cgacgccggc caggccctcg aacagtggat tagccttgcc gatggtcagc tcggcgcggc
      421 cgaactcgcc ggcgtgacca ccctcgacct tgccgcccag cacgtcgcac agcagttgct
      481 ggccgtagca gatggccaga agcggcacgc ccaggtcgaa cagcttggcg ccaatgcggg
      541 ggctgtcggc ttccagcacg ctggccgggc cgcccgacag gatgatggcc gacggagcgt
      601 agtcgtcaac gatggcctcg gccttgtcga acggatggat ctcgcaatag acgcccgcct
      661 cgcgcacccg gcgggcgatc agctgggtga cctggctgcc gaaatcaacg atcagaacgc
      721 gctggtgatg gacgggggcg gtcatgggcg atgggtctcc ggcgttcgaa tgtcttcctt
      781 ctccccttgc gggagaaggt gtcggccatg gccgacggat gaggggtcgc accaacgtcg
      841 gacggtcggc cgctggtcga gcgccgggaa acccctcatc cgaccgctac gcggccacct
      901 tctcccgcaa ggggagaagg gtttctattg tcgttccagc acggcggcga agaagccgtc
      961 cgtaccagcg gtgtgaggtg tcaggcgcag gtagccttgg gcgctgacat gttcggctat
     1021 ggcgtccgag gtgatcggct tgaccgcaaa gtcggggcgg cgctccagga aggctgtgac
     1081 ccgatcctcg ttctcctcga ccagcaggga gcaggtcaca tagacgagcc ggccgccggg
     1141 cttcacgaaa tcggcggcgt cgatcagcac gctgtcctgc tcnnnnnnnn nngttggtcg
     1201 ggcgacaaac gccatttcgc atcaggatgg cggcgccagg tgcccgagcc ggtgcagggg
     1261 gcgtcgacga agacgacgtc cagcttgctc tccagaccct tcagcggctc ggcctcgatc
     1321 ggcgagcgga tctgcaggtt gcgaaccccc gccctctggc cgcgacggat cgtgtcggcc
     1381 aggcggcgcg cgtcgctgtc atgggcgaag atctggccgg tgttgttcat cgccgccgcc
     1441 agggccagcg tcttgccgcc gccgccggcg cagaagtcca ggacctgctt gcccttgatc
     1501 tcgccggcca tggtggcggc gatctgggag cccaggtcct gcacctcgaa ccagcccttg
     1561 gagaaggccg ggaccgcttc caccgatggg gtgcgatccg ccgcttgagg cgcgggaatg
     1621 cggaaggcgg tgtcgagcac gccggccggc tcgccgccga tcagcgcgac ggcttttgcg
     1681 ccacgctcgg cgtcggtctt gatggtattg atgcgcaggt cgaccggggc ccgttgcgac
     1741 agggccgcca tctcgacgcg aacggtgtcg cccagggcgc gagccaggaa gggctccatc
     1801 cagtcgggat agtcgccggc caccggcgcc ggggcgtcat cgagcgaggc cggggcggcc
     1861 agagccttga tctcggcttc ggcaagcgcg ccagcgccgc gctcttcacc cgccgcctcg
     1921 gcgacgcgct cgggcgacca gccccagaca tgggccagaa cccccagcac cacgccgcgc
     1981 gcggagctgt cacccatcat gaagcccagc gaacgcttgc gacgcagggc gtccagcacc
     2041 aggcccgaca cgaaggcccg gtccttggag ccggcatagc gcgcgctttc gccccagcgc
     2101 ttcagcgcca tcttcacggg aaaatgccgc gcctcgatct cggccagaac ctcgatcgcg
     2161 gctgaaaccc gtcctccgtc ccgcatggtc tcagaccacc atggccgcga ggaccatccc
     2221 gatgccgacg aaggaaatca gccagacgat gctgcgcagg acgggaacgc cgaacgcata
     2281 cagcggcatg tagagcgcgc gcgccgccac atagatcagc gcgccatgag cggtcaggta
     2341 accaatcagg cccgacagat aggtgacgat cacggcggcg gcgaacagcg ggaacgtctc
     2401 gcggaaattg gcggaggcgc gttcaagacg ccnnnnnnnn nnggctgcgc ggcggcgctg
     2461 gcccacagca gatggaccaa tccgatcagg accgcgatgg cgaggagctg aagttcgatg
     2521 gcgacccgca tgatctagac cgcgctcgga tagttgggcg cctcgcgcgt gatcatcacg
     2581 tcgtggacgt ggctttcccg cagaccagcg ccggtgatgc gcacgaagcg ggcgcggctc
     2641 tggaactcgg cgatcgtggg ggcgccgaca tagcccatgg cggcgcgaag accgccgacc
     2701 agctggtgca gaaccggggc gatggcgccc ttgaacggcg tctggccctc gatgccttct
     2761 ggcaccagct tgaagctgtc ggtcacttcc ttctggaagt accggtcagc cgagccgcgg
     2821 gccatggcgc ccaccgagcc catgccgcga tagctcttgt acgagcggcc ctggtacagg
     2881 aacacctcgc cgggcgcctc ttcggtgccg gcgaacatcg agcccatcat ggccgtgctg
     2941 gcgccggcgg cgatggcctt ggccaggtcg cccgaatact tgatgccgcc atcggcgatg
     3001 accggcgttc cgctttccct ggcggcgcga gcggcctcca tgatcgcggt cagctgcggc
     3061 acgcccacgc cggcgacaat gcgggtcgtg cagatcgagc cggggccaat gcccaccttc
     3121 accgcatcgg cgccggcatc gattaacgcg cgcgcggcgt cataggtcgc gatgttgccg
     3181 gcgacgatct ggacgcggtt ggcttcgcgc ttcagacggc tgacggcctg ggcgacctgg
     3241 ttggagtgac cgtgggcggt gtcgatgacg acgacgtcga caccggcgtc caccagaccc
     3301 atcgagcgct cgaagccagc gtcgccgacg gtcgaggccg cgccgaccag cagccggccc
     3361 ttgtcgtcct tggcggccag ggggtgagcc tgggccttct cgatgtcctt gacggtgatc
     3421 aggccgacgg cgtggaagcc gtcatcgacc acgatcaggc g
//
LOCUS       DAOMEO010000219         3457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3093_length_3457_cov_3.454145, whole genome shotgun sequence.
ACCESSION   DAOMEO010000219 DAOMEO010000000
VERSION     DAOMEO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3093_length_3457_cov_3.454145"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..898
                     /locus_tag="PLH31_06265"
     CDS             293..898
                     /locus_tag="PLH31_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQR88861.1"
                     /translation="MTTVAIIYHSGYGHTAVLADKIAQGVRDAGQTPVLLHIESATQD
                     FGPILEAASKADAIVFGAPTYMGDVSGVFKVFADATSAVFATGGWKDKLAAGFTNSHS
                     FSGDKAHALASLTILAAQHGMNWINLGLAPPSVTAAERGPDTLNRVGSFIGLAAQSDN
                     ASPEITPPAGDRETARLLGERVAKAALRWTQGADVVLDKAA"
     gene            1078..1878
                     /locus_tag="PLH31_06270"
     CDS             1078..1878
                     /locus_tag="PLH31_06270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR88862.1"
                     /translation="MARRLALITGASAGIGAAFARILASHGYDVALTARRADRLEALA
                     AEIRLRFGVEAYAIPADLADPAGVDAVLAGVEAQGRVVDALVNNAGYGLPGTYATTSW
                     ADQGKFLQVMLTSVCELTHKVLPGMVERRFGRIVNVASLAGLVPGAAGHTLYAATKGF
                     LVKFSQSLHLENLSTGVHVSALCPGFTYSEFHDVNGTRAQVSQSLPEWLWLGADEVAA
                     AGYEAAEANRPVCVPGAPNKTIAAIAKLLPDDWALALMANQGGRFRKV"
     gene            complement(1905..2593)
                     /locus_tag="PLH31_06275"
                     /pseudo
     CDS             complement(1905..2593)
                     /locus_tag="PLH31_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
     gene            2680..3369
                     /gene="pyrF"
                     /locus_tag="PLH31_06280"
     CDS             2680..3369
                     /gene="pyrF"
                     /locus_tag="PLH31_06280"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQR88863.1"
                     /translation="MTADPRLIIPLDLPTVDEARAMVERLGDSVSFYKIGLELLATGG
                     MGLAGELKAQGKQVFLDWKLHDIGATVERSARVLAGAGCDLLTVHAEPQVMRSAVLAR
                     GASDLKILAVTVLTSLTDADLAEMGYAFGARDLVERRIRQAIDCGVDGVVSSPHEAAL
                     AREIAGPDFLVVTPGVRSDWSAKNDQARAATPADALRAGASHLVCGRPITAATDPREA
                     ALKVIAEMAGV"
BASE COUNT          572 a         1238 c         1118 g          529 t
ORIGIN      
        1 gcgggcgtcc cggaagactg gcgcagctta cgacaaatgc tcacagacaa ggggtcgcac
       61 tgccgaccgc ccacagtcca aaccgaaagc cgtttcgcgg ctgattctac cttcaacccc
      121 tcatccgacc tgcttcgcag gccaccttct cccgcagggg gataaggaac caacgcccct
      181 aaatcccctc tcagaaaaac tgcaccaatc tttgttgctg atcctctggc gagtctgaaa
      241 agcgacatta cgtaatcgct acaggttcag tttctaaaca ggggatttcc tcatgaccac
      301 ggtcgccatc atctatcatt ccggttacgg ccacaccgcc gtcctcgccg acaagatcgc
      361 ccagggcgtc cgtgacgccg gccagacccc ggtgctgctg catatcgaaa gcgccaccca
      421 ggacttcggt cccatcctcg aagccgccag caaggccgac gccatcgtct tcggcgcgcc
      481 cacctacatg ggcgacgtct cgggcgtgtt caaagtgttc gccgacgcca cctcggccgt
      541 gttcgccacc ggcggctgga aagacaagct ggccgcgggc ttcaccaatt cgcacagctt
      601 ttccggcgac aaggcccacg ccctggccag cctgacgatc ctggccgccc agcacggcat
      661 gaactggatc aatctgggtc tggccccgcc ttcggtcacc gccgccgaac gcggccccga
      721 tacgcttaac cgcgtcggct cgttcatcgg cctggcggcc cagagcgaca acgcctcgcc
      781 cgagatcacc ccgccggccg gcgaccgcga gaccgcccgc ctgctgggcg agcgcgtcgc
      841 caaggccgcc ctgcgctgga cgcaaggcgc cgacgtcgtg ctcgacaagg cggcttaagg
      901 cccactcgct cgttgcgttc tccccggcga gttgagccgt cacgagcgtg agcccgcgcc
      961 gtgatccgaa ggggcgccga tcccccggcg ccgcttcccc ctttcggttc tcttgaacca
     1021 aatcggctgg cgtgctggtc tccggcggga tttcgctttc gcattggata ccgcttcatg
     1081 gcccgccgtc tcgccctgat caccggcgcc tccgccggca tcggagccgc cttcgcccgt
     1141 atcctggcca gccatggcta tgacgtagcc ctgaccgcgc gccgcgccga ccgcctggaa
     1201 gccctggcgg ccgagatccg cctgcgcttc ggtgtcgagg cctacgccat ccccgccgat
     1261 ctggccgacc ccgccggggt ggacgccgtg ctggccgggg ttgaggctca gggccgggtg
     1321 gtcgacgcgc tggtgaacaa cgccggctac ggtctgcccg ggacctacgc caccaccagt
     1381 tgggccgatc agggcaagtt cctgcaggtg atgctgacct cggtctgcga gctgacccat
     1441 aaggtgctgc cgggcatggt tgagcggcgc tttggccgca tcgtcaatgt cgcctcgctg
     1501 gcgggcctgg tccccggcgc ggccggccat accctgtatg cggcgaccaa gggcttcctg
     1561 gtgaagttct cccagagcct gcatctggag aacctgtcca ccggcgtgca cgtcagcgcc
     1621 ctgtgcccgg gcttcaccta ttcggaattc cacgatgtca acggcaccag ggcccaggtc
     1681 agccagtccc tgcccgaatg gctgtggctt ggggccgacg aagtggccgc cgccggctac
     1741 gaagccgccg aggccaaccg cccggtctgc gtgcccggtg cgcccaacaa gaccatcgcg
     1801 gcgatcgcca aactcctgcc ggacgactgg gcgctggcct tgatggccaa ccagggcggg
     1861 cggttccgga aggtctgaat ccttctcccc ttgcgggaga aggatcactt ggcttccgcg
     1921 taaggggccg ccgcgtccgg tcgcaccagg ccacgcgtgg cgtcgccgat gccgaccagg
     1981 atgaactgca ccgccagggc gcacaggatc aggcccagta cccgcaccac gatgctcatg
     2041 ccgatccggc ccagaacctt gctgatcagc ggcgaggccc agaaggtcag caccgtcacc
     2101 agcgacaccg ccgcgatcac cgccacgccc agggccatgc cggccgcgcc gaacggcttg
     2161 gcctcggcgg cgtagatgat cacggtcgag atcgagcccg ggccgatcag cagcggcatg
     2221 gcgaacggca cgatcagccg ctcgaaccgc gccttggcgt agacccgcgg cgactggccc
     2281 tcgatgccct cggccgcgtc ggcgaacatg gtggtgaagt caccatgtcc aggcccagaa
     2341 tgaacaggat gatgccgccg gcgatgcgga aggccggcat cgagatgccg aagaaggcca
     2401 gcaggcccac cccggtgaaa tagaagaaga ccagaaaggt caccatgaac aggccgatat
     2461 agacggccac catgcggcgg ccggcggcgg ccgcgcccag cgtggcggcg gcgaacagcg
     2521 gcacgttgcc gatcgggtcg atcagggcga acagggcgac gaagaagttg acggcaagtg
     2581 tggcctcggt cattcgacgt ccctaccttt caacgcgttt gaagcttcac cctagttcct
     2641 agggcggacg cgcacgcgcg ccaacccctg ggggtaacca tgaccgccga tccgcgcctg
     2701 atcatcccgc tggacctgcc caccgtcgac gaagcccgcg ccatggtcga gcggctgggc
     2761 gacagcgtca gcttctacaa gatcgggctg gagcttctgg cgaccggcgg catgggccta
     2821 gccggagagc tgaaggccca gggcaagcag gtcttcctcg actggaaact gcacgacatc
     2881 ggcgcgaccg tggagcgctc ggcccgggtg ctggccggcg ccggctgcga cctgctgacc
     2941 gtccacgccg agccgcaggt gatgcgctcg gccgtgctgg cgcgcggcgc ctcggacctg
     3001 aagatcctgg cggtcacggt gctgaccagc ctgaccgacg ccgatctggc cgagatgggc
     3061 tacgccttcg gggcccgcga tctggtcgag cggcgcatcc gccaggccat cgactgcggc
     3121 gtcgacggcg tggtctccag cccgcacgag gcggccctgg cccgcgagat cgccggtccg
     3181 gatttcctgg tggtgacgcc gggcgtgcgc tccgactggt cggcgaagaa cgatcaggcc
     3241 cgcgccgcga cgccagccga cgccctgcgc gccggcgcca gccatctggt ctgcgggcgc
     3301 ccgatcacgg cggcgaccga cccgcgcgag gctgcgctga aggtgatcgc ggagatggcg
     3361 ggggtttgat gcggtagctg ctccccctct gggggagctg tcgcggagcg actgaggggg
     3421 ccatctcggc ataagccgtg ctgaccccct ccggccc
//
LOCUS       DAOMEO010000220         3451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3099_length_3451_cov_2.121614, whole genome shotgun sequence.
ACCESSION   DAOMEO010000220 DAOMEO010000000
VERSION     DAOMEO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_3451_cov_2.121614"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLH31_06285"
     CDS             <1..182
                     /locus_tag="PLH31_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQR88864.1"
                     /translation="LYLKQVLLTMPPLYRDVFVLSRFDGMTYPEIALAHGISLATVER
                     RMAKAVEHCMAQLDV"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            473..711
                     /locus_tag="PLH31_06290"
                     /pseudo
     CDS             473..711
                     /locus_tag="PLH31_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
     gene            864..1139
                     /locus_tag="PLH31_06295"
     CDS             864..1139
                     /locus_tag="PLH31_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88865.1"
                     /translation="MASETSWTAGRLVFAGDTVEAAVAEVNRYSRTKVVLEAPTIARI
                     AVSGAFNTGDVDGFVSALTELYPVVAERTPTGQIVIRDAPTKNASVR"
     gene            1210..1935
                     /locus_tag="PLH31_06300"
     CDS             1210..1935
                     /locus_tag="PLH31_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR88866.1"
                     /translation="MRFGLCSSALSALAIAAVTPLVAQAQSPAVQFRVPAGPLQTALP
                     LFAAQSGEQILYSTDLVAGRQFPGVNGALGTDQALTALLQGTGLRARRTSYNTLVVFD
                     PTARAEATDEAVEIAEVIVTGSLIRGVADGPSPVVTVTRDDIDRQGHGTVAQALAALP
                     QNFGGTGNDAAMSNGGDRTGTNSFYASGVNLRGLGSDATLVLVNGRRMAGTGNKGDFA
                     DVSTIPTSAVARIDVLLDGASAL"
     gene            1967..2179
                     /locus_tag="PLH31_06305"
     CDS             1967..2179
                     /locus_tag="PLH31_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88867.1"
                     /translation="MADGYKGLGQIALFPSSDDLHDSTWASWEYLGLYRRIVWGQMKG
                     SDQDIWMVLNHKANTATPLRKWLTGG"
     gene            complement(2180..2707)
                     /locus_tag="PLH31_06310"
     CDS             complement(2180..2707)
                     /locus_tag="PLH31_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HQR88868.1"
                     /translation="MLAFALALDSALSALTDRFDIFLSHSVRDASLVMGVKRILEEAG
                     KTVYVDWISDPNLDRNAVTGATAERLRHRMRKCDACFYLFSRHSRRSRWMPWELGFFD
                     GHNGNVAILPLIPPTGELDFTGEEYLEIYPKIDFANLSGSPSIFVNQVRRTEMGSYKS
                     YDEWRRGADKLRPTT"
     gene            3039..3451
                     /locus_tag="PLH31_06315"
                     /pseudo
     CDS             3039..3451
                     /locus_tag="PLH31_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611472.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          644 a         1071 c         1098 g          628 t
ORIGIN      
        1 gcctctacct caagcaggtc ctcctgacga tgccgcccct ctacagggac gtcttcgtct
       61 tgagccggtt tgacggtatg acatatccgg agattgctct ggctcacggg atcagcttgg
      121 ctaccgtcga acggcgcatg gcgaaggctg ttgagcactg catggcgcaa ctggacgtct
      181 aaggcacgaa cgatgactgc cgataaagac ctggacgcga gacgtcgtga agccgcgtca
      241 tggttcgcga ccctcaatca gaagcgtgtg acggcctcgg acgtcacagc cttnnnnnnn
      301 nnnaacgatg tgggagacgg cgggcagcct caaaggggat gccgacatcg ccgtcttgac
      361 cgaaggtgcg cgcgctcgcg ctgacgcgtc gcgcaaaacc caaagccgat tctccaaagc
      421 gctcgtaccg accggcgcca tcgccgcgat cgcggccgca gcgatcggcg tcatgatatg
      481 gtccgcccgt ccgctgtctt atgagacggg gctgggagaa cgacgggtcg tgatcctggc
      541 cgacggctcg aaggtcacgc tggacaccga gtctcgcatc accgtgaaac tcacccgtgg
      601 ccgccgcgct gtcgaattgg cgagcggcca ggcctttttg acgtcgaagg cgatccgacc
      661 cgtccgttcg tcgtccgcgc gggggtcacc gacgtcaccg ccgtcggaac ccggttcgac
      721 gtccgccgag cgggcgacgg cgcgcaggtc accctcgtcg agggcaaggt cgatgttctg
      781 gaccgagagg gctcgtcgcc gcgctggtcc ctcacgcctg gccagcagat tgtcaccgcc
      841 gtcccgccgt acgatcggtg gatgtggcgt cggaaaccag ctggacggcc ggtcgtctcg
      901 tcttcgcggg cgacaccgtt gaagccgccg tcgccgaggt caatcgctac agccgcacca
      961 aggtcgtgct ggaagcgccg accatcgcgc gcatcgccgt cagcggcgcg ttcaacacgg
     1021 gtgatgtgga cggcttcgtc tccgccctca ccgaactcta tcccgtcgtc gccgagcgga
     1081 cgcccactgg tcagatcgtc attcgtgacg cgcccacaaa aaatgcgagt gttcgctgag
     1141 gggttttgcc gcgccccttc gtctcttccc cgacccgcag cactgcggat atccatttcg
     1201 ggggttttta tgcgtttcgg tctttgttcg tctgcgctga gcgcactggc tatcgccgcg
     1261 gtgacgcctc tcgtcgccca ggcgcaatcc cctgccgtgc agttccgtgt tcccgccggc
     1321 ccgctgcaaa ccgcactgcc cctgttcgcg gcccaaagcg gcgagcaaat tctctactcc
     1381 accgacctcg tcgcaggccg ccaattccct ggcgtcaatg gcgcgcttgg gacagaccaa
     1441 gccctcaccg ccctgctgca gggcaccgga ctacgggccc ggcggacgag ctacaacacg
     1501 cttgtcgtct tcgatcccac cgcccgtgca gaagcgacgg acgaggcggt cgagatcgcg
     1561 gaagtgatcg tcactggcag tttgatccgc ggcgtcgctg acggcccgtc gccggtcgtc
     1621 accgtgaccc gggacgacat cgaccggcaa gggcatggaa cggtcgccca agcgcttgcc
     1681 gccctgcccc agaactttgg cggcaccggt aacgatgccg ccatgagcaa cggcggcgac
     1741 cgaacgggta cgaacagctt ttacgcttcg ggcgtcaatc tgcgcggctt gggcagcgat
     1801 gctactctcg ttctggtcaa cgggcgacga atggcgggca caggaaacaa gggcgacttc
     1861 gccgatgtct ccacaatacc gaccagcgcc gtggcccgca tcgacgtgct gctcgatggt
     1921 gcgtcagcgc tttaaggagt cgcgctgggt gccttgggag ctggaagtgg ctgacggcta
     1981 caagggcctc ggacaaatcg ctctgtttcc gtcctcggac gatctgcacg actcgacctg
     2041 ggcctcatgg gaatatctgg gcctctatcg ccggatcgtt tggggccaga tgaagggcag
     2101 cgaccaagac atctggatgg tactaaatca caaagcgaac accgcaacgc ctctccggaa
     2161 atggctgacg ggtgggtagc taggtcgtgg gtctaagctt atcagcgcca cgccgccatt
     2221 catcatagga cttgtacgac cccatctccg tgcgtcgtac ttggttcacg aatatgctgg
     2281 ggctgccgct taggtttgcg aagtctatct tgggataaat ctctagatac tcttcgcctg
     2341 tgaagtcgag ttcgcccgtt ggagggatca gcggaaggat cgcgacgttg ccgttatggc
     2401 cgtcgaaaaa accgagctcc cacggcatcc acctcgaccg cctcgaatgt cgggaaaaga
     2461 gatagaagca ggcgtcgcac ttcctcatac gatgccgcaa cctctctgcc gtagcgccgg
     2521 tgactgcgtt tcggtcaagg ttcggatcgc tgatccagtc cacgtagacg gtctttccgg
     2581 cctcctccag gatccgcttc acccccatga cgagactagc gtcccgaacg gaatgggaga
     2641 ggaaaatgtc gaagcgatct gtcagggcgc tcagcgcgga gtcgagagcg agcgcgaacg
     2701 ccagcacatg agcgggctcg tcctctgcta cgccagcgcg agcgcgggct tgagcttcag
     2761 ttagatacat acggtcaagc tgaagcgtag acccgcccgt gtccaattgg agattgagta
     2821 ggcatcggaa gtccggggtt cagcaactcg aagcggtttg acgccctgct ccccgaagcg
     2881 cgatcttgtt tttgaaggac ctgccgccgg gaggccgaca atgcagcggc cttcattgcg
     2941 ctgggtactg cggagagcgc cacaagcgaa ccaagatgac gtgccccctt cctgatccct
     3001 cgatttgagt gagagtccgt ttcatcaagg agacggacgt gaagaagagc aggttcaacg
     3061 aggcgcagat catcggcgtg ctgcgcgagc aggaggccgg tagcccgacg gcggaggttt
     3121 gccggcggca cgggatcagc gagcagacct tctatcggtg gaaggcgaag tacagcggca
     3181 tgagtgtgtc ggacgcccag aagctgaaga cgctggaaga cgagaaccgg cggctgaaga
     3241 agctgctggc ggagtcgatg ctggacgtgt cggcgctgaa ggatcttctg ggaaaaaact
     3301 gatcgggcct gcagcgcgca gagcggccgt gcttcgcctg atggcggagc gcggcttctc
     3361 ccagaagcgc gcctgcgggc tggttcagat cgatccaaag acggtgcgtc gcgagcctga
     3421 accgggcgac gtggaggtgc gcgagcgcct g
//
LOCUS       DAOMEO010000221         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3116_length_3434_cov_2.972477, whole genome shotgun sequence.
ACCESSION   DAOMEO010000221 DAOMEO010000000
VERSION     DAOMEO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_3434_cov_2.972477"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /gene="uvrA"
                     /locus_tag="PLH31_06320"
     CDS             <1..1912
                     /gene="uvrA"
                     /locus_tag="PLH31_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQR88869.1"
                     /translation="ARHYGFSMDEPWHKLAASARDIVLFGSKGEKIKFTYDDNARKYE
                     VAKPFEGVLPNLERRWRETDSSWVREELGRYQSDTPCEVCHGKRLKPEALAVKIAGMD
                     IAQVSMLAIRPAKDWFAGLEAQFSDKQMEIARRILKEINDRLRFLVDVGLDYLNLSRG
                     SGTLSGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNTRLLTSLQGLRDLGNSV
                     LVVEHDEEAILTADYVIDMGPAAGVHGGEIVAEGKPADIMANPASITGQYLTGAREIE
                     VPEDRRPINKKKMLKVIGATGNNLKNVTGEIPVGTFTCITGVSGGGKSTFTIETLYKA
                     AARRLNNASDAPAPHERIEGLENFDKVIDIDQSPIGRTPRSNPATYTGAFGPIRDWFA
                     QLPESKARGYGPGRFSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCDVCKGKRYNRE
                     TLDILFKGKTIAEVLDMTVEEAADFFKAVPPIRDKMETLKRVGLTYIKVGQPATTLSG
                     GEAQRVKLSKELSKRATGRTLYILDEPTTGLHFEDTKKLLEVLHELVDQGNTVVVIEH
                     NLDVVKTADWLLDFGPEGGDGGGEIVAVGSPQDVAKVEASWTGRYLKDVLTRHEDRRK
                     ARVAELKKSKRA"
     gene            complement(1991..>2550)
                     /locus_tag="PLH31_06325"
     CDS             complement(1991..>2550)
                     /locus_tag="PLH31_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88870.1"
                     /translation="LVLVSLTLLGIYFAAVLVSAILLGIGVAAGGGAMTGLAGLISFV
                     GVACLMIYFTIRLSFASTMTFDTGRIAVRASWALTKGHVGAILGAYVLATVMALIVYL
                     LIMTIVAAVGVIVSGGISGISGLGGLFEPDMTSLETFFTPIGLARAAFAGVISVLTSL
                     IMFSPAPEIYRILKAGGAEPVAPSV"
     assembly_gap    2551..2560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2561..2848)
                     /locus_tag="PLH31_06330"
     CDS             complement(<2561..2848)
                     /locus_tag="PLH31_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88871.1"
                     /translation="MSKFSASDAAFVGFRLVREHPRTVAVWAVLMTVLSLMTSTLTIK
                     LAGPQLTEFMALSADNEASPDAMFKVMGGLLPLMVVSMVYSVALYSVMLAAV"
     gene            3025..>3434
                     /locus_tag="PLH31_06335"
     CDS             3025..>3434
                     /locus_tag="PLH31_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR88872.1"
                     /translation="MKLYGEANPAPNPRRVRIFLAEKGVDLPEIRVGMRQGGHKSPEH
                     KARNSLGQVPTLELEDGTTISESVAICRYLEALHPEPPLFGTTPLEQAQIDMWVRRIE
                     FQLLIPVGMFWRHAHPLTAALLKQNVEFGESNRET"
BASE COUNT          683 a         1151 c         1112 g          478 t
ORIGIN      
        1 ggcccgccac tacggcttct cgatggacga gccctggcac aagctggcgg ccagcgcccg
       61 cgacatcgtg ctgttcgggt ccaagggcga gaagatcaag ttcacctatg acgacaacgc
      121 ccgcaaatac gaggtcgcca agcccttcga gggcgtgctg ccgaacctgg aacgccgctg
      181 gcgcgagacc gacagcagct gggtgcgcga ggagctgggc cgctatcagt ccgacacccc
      241 ctgcgaggtc tgccacggca agcgcctgaa gcccgaggcc ctggcggtca agatcgccgg
      301 catggacatc gctcaggtct cgatgctggc catccgtccg gccaaggact ggttcgccgg
      361 tctggaagcc cagttcagcg acaagcagat ggagatcgcc cggcggatcc tgaaggagat
      421 caacgaccgc ctgcggttcc tggtcgatgt cgggctggac tatctgaacc tgtcacgcgg
      481 ctcgggcacc ctgtcgggcg gcgagagcca gcgcatccgt ctggccagcc agatcggctc
      541 gggtctcacg ggggtgctct acgtgctgga cgagccgtcg atcggcctgc accagcgcga
      601 caacacccgc ctgctgacct cgctgcaggg cctgcgcgac ctcggcaatt ccgtgctcgt
      661 cgtcgagcac gacgaggagg ccatcctcac cgccgactat gtgatcgaca tgggcccggc
      721 ggccggcgtg cacggcggcg agatcgtcgc cgagggcaag cccgccgaca tcatggccaa
      781 ccccgccagc atcaccggcc agtacctgac cggcgcgcgc gagatcgagg tgccggaaga
      841 ccgccgcccg atcaacaaga agaagatgct gaaggtcatc ggcgccacgg gcaacaacct
      901 gaaaaacgtg accggcgaaa tcccggtcgg caccttcacc tgcatcaccg gcgtgtccgg
      961 cggcggcaag tcgaccttca ccatcgagac cctctacaag gccgccgccc ggcgtctgaa
     1021 caacgccagc gacgccccgg ccccgcacga gcgcatcgag ggtctggaga acttcgacaa
     1081 ggtcatcgac atcgaccagt cgcccatcgg ccgcaccccg cgctcgaacc cggccaccta
     1141 taccggcgcc tttggcccga tccgcgactg gttcgcccag ctgccggaga gcaaggcgcg
     1201 cggctatggc cccggccggt tcagcttcaa cgtcaagggc ggccgctgcg aggcctgcca
     1261 gggcgacggc ctgatcaaga tcgagatgca cttcctgccc gacgtctacg tcacctgcga
     1321 tgtctgcaag ggcaagcgct acaaccgcga gactctcgac atcctgttca agggcaagac
     1381 catcgccgag gtcctcgaca tgaccgtcga ggaggctgcc gacttcttca aggcagtgcc
     1441 gccgatccgc gacaagatgg agacgctcaa gcgcgtgggc ctgacctata tcaaggtcgg
     1501 ccagccggcg acgacgctgt cgggcggtga agcccagcgg gtgaagctgt ccaaggagct
     1561 gtccaagcgc gccaccggcc gcacgctcta catcctcgac gaaccgacca ccggcctgca
     1621 cttcgaggac accaagaagc tgctggaagt gctgcacgag ctggtcgacc agggcaatac
     1681 cgtggtggtc atcgagcata acctcgatgt cgtgaagacc gccgactggc tgctggactt
     1741 cggccccgaa ggcggcgacg gcggcggcga gatcgtcgcc gtgggctcac cccaggacgt
     1801 ggccaaggtc gaggcctcct ggaccgggcg ctatctgaag gatgtgctga cgcgccacga
     1861 ggaccgtcgc aaggcgcggg tggcggagct gaagaagagc aagcgggcct agtcgaggtg
     1921 atcgtttcgc cttgaagcga tcaccaaaaa ctccgctctc acccggccaa cgccggggag
     1981 agcggaaagg tcagacgctc ggcgccaccg gctccgcccc acctgccttc aggatccggt
     2041 agatctcagg ggctggcgag aacatgatca gcgacgtcag gaccgagatc acaccggcga
     2101 acgccgcgcg ggccaggccg atgggggtga agaaggtttc cagcgaggtc atgtccggct
     2161 cgaacaggcc gcccaggccg ctgatgccgc tgatgccgcc cgagacgatc acgcccacgg
     2221 ccgcgacgat agtcatgatc agcagataga cgatcagcgc catcaccgtg gccaggacat
     2281 aggcccccag gatcgcgccg acatggccct tggtcagggc ccatgaggcg cgaacggcga
     2341 tccggccggt gtcgaaggtc atggtcgagg caaaggacag gcggatcgtg aaatagatca
     2401 tcaggcaggc cacgccgacg aagctgatca ggcccgccag gcccgtcatg gccccgccgc
     2461 ccgccgccac gccgatcccc aacagaatgg ccgaaaccag gacggccgcg aaatagatcc
     2521 ccagaagcgt caggctcacc aggaccagcg nnnnnnnnnn caccgccgcc agcatcaccg
     2581 aatacagcgc cacgctgtag accatcgaca cgaccatcag cggcagcaag ccgcccatga
     2641 ccttgaacat cgcgtcgggt gaggcttcgt tgtcggcgct gagggccatg aactcggtca
     2701 gttgcgggcc ggccagcttg atggtcagcg tgctggtcat cagcgacagc accgtcatca
     2761 gcacggccca caccgccacg gtcctcggat gctcccgcac gagccggaag ccgacgaagg
     2821 cggcgtcgga ggcggagaac ttggacatgc ggccctctgg gacggaacga gacaagcgct
     2881 acgcctaaca aggttcgcgg cgtttcgaca gggcgtggcg gccgaaatcg acggtgaaac
     2941 gaatgcggct tgccggttgc gccccggcaa ttctaacatc tgtttgaaag cggccgtcag
     3001 cgtcgctgaa tccgggaggt ctcgatgaag ctctatggcg aagccaaccc cgcgcccaat
     3061 ccgcgccggg tgcgcatttt cctggccgaa aagggcgtag acctgccgga gatccgggtc
     3121 ggcatgcggc agggcggcca caagtcgccc gagcacaagg cgcgcaacag cctgggccag
     3181 gtgccgaccc tggagctgga ggacgggacg accatcagcg agagcgttgc gatctgccgc
     3241 tatctggagg cgctgcaccc agagccgccg ctgtttggaa ccacgccgct ggaacaggcc
     3301 cagatcgaca tgtgggtgcg gcgcatcgag tttcagctgc tgatcccggt cggcatgttc
     3361 tggcgccatg cccatccgct gacggcggca ctgctcaagc agaacgtcga gtttggcgag
     3421 tccaaccgcg agac
//
LOCUS       DAOMEO010000222         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3117_length_3434_cov_2.436520, whole genome shotgun sequence.
ACCESSION   DAOMEO010000222 DAOMEO010000000
VERSION     DAOMEO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_3434_cov_2.436520"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLH31_06340"
     CDS             <1..982
                     /locus_tag="PLH31_06340"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQR88873.1"
                     /translation="RANTVYEGQYLLGTSIARPLIAKKQIEIARKTGADAVSHGATGK
                     GNDQVRFELGYYGLEPDITVIAPWREWDFKSREALLEFAEKHQIQITKDKRGEAPFSV
                     DANLLHSSSEGKVLEDPAVEAPEFVHMRTIAPEDAPDKPHVFTLDFEKGDPVAIDGVA
                     MSPATLLTKLNELGRDNGVGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITL
                     DRGAMHLKDELMPKYASLVYNGFWFAPEREMLQAAIDYSQTKVSGQVRVKLYKGNVSI
                     IGRTSPYSLYDQDLVTFEEGKVAYDHRDAGGFIKLNALRLRVLAKRDQRD"
     gene            complement(1027..1554)
                     /locus_tag="PLH31_06345"
     CDS             complement(1027..1554)
                     /locus_tag="PLH31_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88874.1"
                     /translation="MQLAVSALAALIVMGSAAASPARAIDLAPAPKVFIKDGESYRWQ
                     VAHGVTGGLILSGPLRADGSNIDLVITCSGLRSGGVQARFYEPKPGASQLRLRTATSV
                     FRVPRGAQTYEARAFLEGRGDVPRNFFKALARTPTISVEYAGQSTDFAGPGVALTEHF
                     QRYCSGLTRRAAADE"
     misc_feature    1711..1764
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03058"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="sul1 is cis-regulatory element that is thought to
                     sense ions involved in sulfur or methionine metabolism;
                     They are found in Alphaproteobacteria; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03058"
     gene            1819..3303
                     /locus_tag="PLH31_06350"
     CDS             1819..3303
                     /locus_tag="PLH31_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQR88875.1"
                     /translation="MTLLASARQQWLANPRRDLLAGTVVALALIPEAIAFSIIAGVDP
                     AVGLYASFIIAVTIALVGGRPAMISAATGAMALLMVTLVRDHGLEYLFAASLLCGVLQ
                     IIIGLLKLGRYIKFVSKSVMTGFVNSLAILIFLAQMPELIGANWQTFAMVGAGLAIIY
                     GFPLITKAVPAPLVAIIALSAFAIVTGLDVRTVGDMGQMPSSLPAFHLPAVPLTWDTL
                     RIIGPISATLAFVGLLESLLTANLLDDITDTPSDKDRETRGQGIANIAASLFGGMAGC
                     AMIGQSIINVTSGARGRLSTLWAGLFLLFLILVLQDWVARIPMPALVAVMIMVSVGTF
                     DWKSVLNLRSTPVQSSVVMIATTLTVVFTHDLSKGVVLGVILSAVFFMRKVGKTIAVT
                     EIETPEEGVLRYKVTGQLFFASADLFAASFEHHGHPTRVEIDMTDAHLWDLTGVAAVD
                     KVVFRYRRQGAQVEVIGMNAAGQTLVDRVGKSDKSHLPAGGAAH"
     gene            complement(3317..>3434)
                     /locus_tag="PLH31_06355"
     CDS             complement(3317..>3434)
                     /locus_tag="PLH31_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HQR88876.1"
                     /translation="AYVYVGVPEKVSVAFVRSASKGRFFQRVIRDRYPYNRI"
BASE COUNT          603 a         1126 c         1106 g          599 t
ORIGIN      
        1 ccgcgccaac acggtctacg agggccagta cctgctgggc acctcgatcg cccgtccgct
       61 gatcgccaag aagcagatcg agatcgcccg caagaccggc gccgacgctg tcagccacgg
      121 cgcgaccggc aagggcaacg accaggtgcg gttcgagctg ggctattacg gcctggaacc
      181 cgacatcacc gtgatcgctc cctggcgcga atgggacttc aagtcccgcg aggccctgct
      241 ggagtttgcg gaaaagcacc agatccagat caccaaggac aagcgcggcg aggccccgtt
      301 cagcgttgac gccaaccttc tgcactcctc atccgagggc aaggtgctgg aagatccggc
      361 cgtcgaggcc cccgaattcg tccacatgcg cacgatcgcg ccggaagacg cccccgacaa
      421 gccgcacgtc ttcaccctcg acttcgagaa gggcgacccg gtcgccatcg acggcgtcgc
      481 catgtcgccc gccaccctgc tgaccaagct gaacgaactg ggccgcgaca atggcgtggg
      541 gcgtctggac ctggtcgaaa accgcttcgt cggcatgaag tcgcgcggcg tctacgagac
      601 cccgggcggc accatcctgc tggccgccca ccgcggcatc gagagcatca ccctggaccg
      661 cggcgccatg cacctgaagg acgagctgat gccgaaatac gcatcgctcg tttacaacgg
      721 cttctggttc gcccccgagc gcgagatgct gcaggcggcc atcgactaca gccagaccaa
      781 ggtgagcggt caggttcggg tcaagctcta caagggcaat gtctcgatca tcggccgcac
      841 cagcccctac agcctgtacg accaggacct ggtgacgttc gaggaaggca aggtcgccta
      901 cgaccaccgc gacgccggcg gcttcatcaa gctcaacgcc ctgcgcctgc gggtcttggc
      961 caagcgggat cagcgcgact agggcctatc gtgtccggtc ctgcctcggt ggcgggaccg
     1021 gacacactat tcgtccgccg ccgcgcgacg ggtaaggccg gagcaatatc gctggaaatg
     1081 ttcggtgagc gcgacaccgg gacctgcgaa atcggtcgat tgaccagcat actcgactga
     1141 gatcgtcggg gtgcgagcca aggccttgaa gaagtttctg ggcacgtcgc cgcgcccttc
     1201 caggaacgcg cgggcctcat acgtctgagc gccgcgaggc actcggaaca cgctggtggc
     1261 ggtcctgagc cgcagttgcg atgcaccggg cttgggttcg taaaagcggg cttgaacgcc
     1321 gccagagcgc agacccgagc aggtgatgac caagtctatg ttggaaccgt cggcgcggag
     1381 cgggccagac aggatcaggc cgccggtaac gccgtgcgcg acctgccagc gataggattc
     1441 tccatccttg atgaagacct ttggcgccgg cgccaagtcg atagcgcgcg cgggtgacgc
     1501 tgccgctgat cccatgacga tcaaagcggc gagcgcagaa acggccaact gcatgagagc
     1561 ctcccaaggt agaggcatcc taggtcgtgc ataatatcag cacaaccttt gagtgctcac
     1621 ccgggtttcg acctccggtg gcgtcgtggc gaggggctaa aagccgttgc ttgcattgct
     1681 cggcgcatta cggcatcagc agcccgtcac taaggacccg gccagatcgg gtggctaccc
     1741 cgggcgccga tccctcggga aaccgaacgg acctgagcgc gccgcgccaa tgtccgcgag
     1801 cgacagactg aaccctcaat gacccttctt gcttcggcgc gtcagcaatg gctcgccaat
     1861 ccgcgccgcg atctgttggc gggcaccgtc gtggccctgg ccctgatccc ggaggccatc
     1921 gccttctcga tcatcgcggg tgtcgatccc gccgtcggcc tctatgcctc gttcatcatc
     1981 gccgtgacca tagccctggt cggcggacgg ccggcgatga tctcggcggc cacgggcgcc
     2041 atggcgctgc tgatggtcac gctcgtgcgc gaccacgggc tggaatatct gttcgcggcc
     2101 tcactgttgt gcggggtgtt gcagatcatc atcggccttc tgaagctggg acgctatatc
     2161 aagttcgtct ccaagagcgt gatgacgggc ttcgtcaact cgctggcgat cctgattttc
     2221 ctggcccaga tgccggaatt gatcggggcc aactggcaga ccttcgccat ggtcggggcg
     2281 ggtctggcga tcatctatgg cttcccgctg atcaccaagg cggtccccgc gccgctggtg
     2341 gcgatcatcg cgctgagcgc ctttgcgatc gttaccggcc tggacgtccg cacagtgggc
     2401 gacatgggcc agatgccttc gagcctgccg gccttccacc tgccggctgt gccgctgacc
     2461 tgggacacgc tgaggatcat cgggccgatc tcggcgaccc tggcctttgt gggcctgctg
     2521 gaatccctgc tgacggccaa tctgctggac gacatcactg atacgccgtc ggacaaagac
     2581 cgcgagacgc gcggccaggg catcgccaat atcgccgcgt cgctgtttgg cggcatggcc
     2641 ggctgcgcga tgatcggcca gtcgatcatc aatgtgacct ccggcgcgcg gggccggctg
     2701 tcgaccctgt gggcgggcct cttcctgctg ttcctgattc tggtcctgca ggactgggtg
     2761 gcccgcatcc ccatgccggc cctggtggcc gtgatgatca tggtttcggt gggaacgttc
     2821 gactggaagt cggtgctgaa cctgcgctcg acgccggtcc agtcgtcggt cgtcatgatc
     2881 gccacgacgt tgacggtggt cttcacccac gacctgtcca agggcgtggt gctgggcgtg
     2941 atcctctcgg cggttttctt catgcgcaag gtcggcaaga ccatcgccgt gaccgagatc
     3001 gagacgccgg aagagggcgt gctgcgttac aaggtcaccg gtcagctgtt cttcgcctcg
     3061 gccgatctgt tcgccgccag cttcgagcat cacggccatc cgacccgcgt cgaaatcgac
     3121 atgacagacg cccacctgtg ggacctcacc ggcgtggcgg ccgtcgacaa ggtcgtgttc
     3181 cgctatcgcc gccagggtgc gcaggtcgag gtgatcggca tgaatgcggc gggccagacc
     3241 ctggtggaca gggtcggcaa gtccgacaag agccatctgc cggcgggagg agcggcgcac
     3301 tgactgtgcg cgcgcttcag atccggttgt acggatagcg gtcccggatc acccgctgga
     3361 agaaccggcc ctttgaggcc gagcggacga aggctacgct gaccttctcg ggcacgccca
     3421 catagacgta cgcg
//
LOCUS       DAOMEO010000223         3430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3120_length_3430_cov_2.636741, whole genome shotgun sequence.
ACCESSION   DAOMEO010000223 DAOMEO010000000
VERSION     DAOMEO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_3430_cov_2.636741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PLH31_06360"
     CDS             complement(<1..1053)
                     /locus_tag="PLH31_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQR88877.1"
                     /translation="MPQPGRLDQTARESGGRIIAALAAAFRDLDLAEEAYAEACARAA
                     AVWPSRPPHDPAAWLYATARRAALDAVRRRGVRGRLAPDPPQPPPSVEEAMTSDDALI
                     PDERLRLIFVCCHPVVPVEARAPLTLRLVCGLSVREIARAFLVAEPAMFQRLTRAKRR
                     IAQSGEPFEIPGPQAWTERLSAVLSTLEVAYAKAHEDAAGAGAHAGYARDMLALTGAL
                     AELLPNEPECLGLAALVRHAEARRPARLDETGAMVPLSEQDPTLWRKPLMREAERLLR
                     RAAELDRLGPRQLQAAIHAVWCGRKSLAEPPRWAAVLRLYDALLDWRDDAVVRLNRAV
                     ALAEVAGPQAALTEVEG"
     gene            complement(1060..1404)
                     /locus_tag="PLH31_06365"
     CDS             complement(1060..1404)
                     /locus_tag="PLH31_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQR88878.1"
                     /translation="MKYMLLIYENEANYAGEAGEKLLADIIVQHMKLGADLAASGIDW
                     SGSELSPTFTAATVNKDGVVHDGPFAETHEQLGGYYMVDVPDRETAIVWARKIPLSPG
                     GKVEVRPTGGHG"
     gene            complement(1690..3024)
                     /locus_tag="PLH31_06370"
     CDS             complement(1690..3024)
                     /locus_tag="PLH31_06370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQR88879.1"
                     /translation="MTQTFDLIVRGGEVVNHAGRGFTDIGVRGGKIVAIGDLSQASAG
                     EVFDATGLTVLPGVIDSQVHFREPGLEWKEDLESGSRGAALGGVVAVFEMPNTEPTTT
                     DPDALADKLSRAKNRMHTDHAFYVGGTHENATFLGELERLPGCCGIKVFMGASTGSLL
                     VQDDEGVENVLRHVNRRAAFHSEDEYRLADRRHLARPGDWTSHPEVRDAQAALQSTHR
                     LVRIAKSLGKRIHVLHVTTKEEIDFLAQNKDVASVEVTPQHLTLVAPEAYERLKGFAQ
                     MNPPIRSADHVAGVWRGIETGIADVLGSDHAPHTREEKARPYPASPSGMPGVQTLAPI
                     MLTHVVDGRLSLERFVDLTSHGVNRVFGLADKGRIAEGFDADFTIVDMKAKRTITHDW
                     MATRSGWTPFDGFEAKAWPMATIVRGIVVMRDDEIIAEGRGEPVRFLETLAG"
     gene            complement(3095..>3430)
                     /locus_tag="PLH31_06375"
     CDS             complement(3095..>3430)
                     /locus_tag="PLH31_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQR88880.1"
                     /translation="GPLTRAVRGPRAFEQFLAVDPDLSQAIRHMSDLPVATVVKAAKG
                     LLAEAKAAGEAAAVVEAEVEAVALEVAPEPEIEPERPEPVIIMPEPRGQAETEAPDPE
                     SEDAAPSAG"
BASE COUNT          493 a         1210 c         1213 g          514 t
ORIGIN      
        1 cccctcgacc tcggtgagcg cggcctgagg accagccacc tcagccaggg ccaccgcccg
       61 attgagccgc accaccgcat cgtcgcgcca gtccagcagg gcgtcataga gacgcagcac
      121 ggcggcccag cgcggcggct cggcaaggct cttgcgaccg caccagaccg cgtggatcgc
      181 cgcctgcagc tggcgcgggc ccaggcggtc cagctcagcg gcgcggcgca gcagccgctc
      241 ggcctcgcgc atcaacggct tgcgccacag ggtcgggtcc tgctccgaca gcggaaccat
      301 ggcgccggtc tcgtccagcc gggccggtcg ccgcgcctcg gcgtgacgga ccagcgccgc
      361 cagacccagg cactcgggct cgttcggcag cagctcggcc agggcgccgg tcagggccag
      421 catatcgcgg gcatagccgg cgtgggcgcc ggcccccgcc gcgtcctcgt gggccttggc
      481 gtaggccacc tccagggtcg agagcaccgc cgacagccgc tcggtccagg cctggggacc
      541 ggggatctcg aacggctcgc ccgactgggc gatcctgcgc ttggcccggg tcaggcgctg
      601 aaacatggcc ggctcggcca ccaggaaggc gcgggcgatc tcgcgcacgc tgaggccgca
      661 gaccagcctc agggtcaggg gcgctcgcgc ctcgaccggc acgacgggat ggcagcagac
      721 gaagatcagc cgcagccgct cgtcggggat cagggcgtca tcggaggtca tggcctcctc
      781 cacgctgggc ggtggctgcg gcggatcggg cgcgaggcgg cccctcacgc cgcgccgtcg
      841 cacggcgtcc agggccgcgc gacgggccgt ggcgtacagc caggcggccg ggtcgtgagg
      901 tggccgggac ggccagaccg ccgccgcgcg ggcgcaggcc tcggcatagg cctcttcggc
      961 caggtcgagg tcgcggaagg ccgcggccag ggcggcgatg atccgcccgc cgctttcgcg
     1021 ggcggtctgg tcgaggcgac ccggctgagg catcagcgcc tagccatggc caccggtcgg
     1081 ccgcacctcg accttgccgc cgggcgacag cgggatcttc cgcgcccaga cgatggccgt
     1141 ctcgcggtca ggtacgtcga ccatgtagta gccgcccagc tgctcgtggg tctcggcaaa
     1201 ggggccgtca tgcacgactc cgtccttgtt caccgtggcc gccgtgaagg tgggcgacag
     1261 ctctgacccg ctccagtcga tccccgaggc ggccaggtct gcgcccagct tcatgtgctg
     1321 gacgatgatg tcggccagca gcttctcacc cgcctcgccg gcatagttgg cttcgttctc
     1381 gtagatcagc agcatgtact tcatgtcggg tctcccaacc ggcaggcgca cgccgcgccg
     1441 ccttcggggc agagacgcgc gaggtcggcg ggattcgaca gggggatcgg ggaaaagtcg
     1501 aggggggcta cccgacatgg cgtcgcagca agagaatctg tcatcccgga caagcgaagc
     1561 gcagatccgg gaccgcccga agctctgagt tcgggcgtcg gtcccggatc ttcacgctgc
     1621 gcgctcgtcc gggatgacaa cctttttcgg ctataagcga tcgccctgcc tgctaggcag
     1681 aacctaaagt caccccgcca gcgtctccaa aaaccgcacc ggctccccgc gcccttcggc
     1741 gatgatctcg tcatcgcgca tcaccacgat cccccgcacg atggtcgcca tcggccaggc
     1801 cttggcctcg aagccgtcaa acggcgtcca gcccgagcgg gtggccatcc agtcgtgggt
     1861 gatggtgcgt ttggccttca tgtcgacgat ggtgaagtcg gcgtcgaagc cctcggcgat
     1921 gcggcccttg tcggccaggc cgaacacgcg gttgacgccg tggctggtca ggtcgacgaa
     1981 gcgctccagg ctcagccggc cgtcgaccac atgggtcagc atgatcggcg ccagggtctg
     2041 gacgcccggc atgcccgagg gcgaggccgg atagggccgg gccttttcct cgcgggtgtg
     2101 gggagcgtgg tcgctgccca gcacgtcggc gatgccggtc tcgatgccgc gccagacgcc
     2161 cgccacgtgg tcggccgagc ggatcggcgg gttcatctgg gcaaagccct tcaggcgctc
     2221 ataggcttcc ggcgccacca gggtcaggtg ctggggcgtg acctcgacgg aggcgacgtc
     2281 cttgttctgg gccaggaagt caatctcttc cttggtggtc acgtgcagca cgtggatgcg
     2341 cttgcccagc gacttggcga tgcggaccag acggtgggtc gactgcaggg cggcctgggc
     2401 gtcgcgcacc tcggggtggc tggtccagtc accgggacgg gccaggtggc ggcgatcggc
     2461 caggcggtac tcgtcctcgg aatggaacgc ggcgcggcgg ttgacgtgcc gcagcacatt
     2521 ctcgacgccc tcgtcgtcct gcacgagcaa ggacccggtc gaggcgccca tgaacacctt
     2581 gatgccgcag cagcccggca ggcgctccag ctcgccgagg aaggtcgcgt tctcgtgggt
     2641 gccgcccaca tagaaggcgt ggtcggtgtg catgcggttc ttggcccgcg acagcttgtc
     2701 ggcgagcgca tcaggatcgg tggtggtcgg ctcggtgttg ggcatctcga acacggcgac
     2761 cacgccgccc agggccgcgc cgcgcgagcc gctctccaga tcttccttcc actccagccc
     2821 cggctcgcgg aagtgaacct ggctgtcgat gacgccgggc aggacggtga ggccggtggc
     2881 gtcgaagact tcgccggcgc tggcctggct gagatcaccg atagcgacaa tcttgccgcc
     2941 ccgcaccccg atgtcggtaa agccccgccc cgcgtgattg accacctcgc cgccacgcac
     3001 gatcaggtca aaggtctggg tcatgggacg gctcctgcgg agggtttgag gcggtctagg
     3061 tccggccgcc tcgcgaggca atacacttat ccgctcatcc cgcggacggt gcggcatcct
     3121 cggactccgg gtctggcgcc tcggtctcgg cctggccgcg cggctcgggc atgatgatca
     3181 ccggctcggg gcgttccggc tcgatctcgg gttcgggcgc aacctctagc gcgacggctt
     3241 cgacctccgc ctcgaccacc gccgcagcct cgcccgccgc cttcgcctcg gccaacagcc
     3301 ccttggccgc cttgaccacg gtggccaccg gcaggtcgct catgtggcgg atggcttgcg
     3361 acaggtccgg atccacggcc aggaactgct cgaacgcgcg cggaccccgc acggcgcggg
     3421 tcagcgggcc
//
LOCUS       DAOMEO010000224         3425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3125_length_3425_cov_2.713650, whole genome shotgun sequence.
ACCESSION   DAOMEO010000224 DAOMEO010000000
VERSION     DAOMEO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_3425_cov_2.713650"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLH31_06380"
     CDS             <1..406
                     /locus_tag="PLH31_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88881.1"
                     /translation="IGSAGMAVTLGVLTWCFSTATTVNGALTLGDQVGLTALIAANLY
                     VVFFNLSWGPVMWVMLGEMFPNQMRGSALAVAGFAQWIANFLISVSFPALAAISLPMT
                     YGFYAVSALVSFFLVQKLIQETRGKELEDMKG"
     gene            742..2361
                     /locus_tag="PLH31_06385"
     CDS             742..2361
                     /locus_tag="PLH31_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQR88882.1"
                     /translation="MVQISRRGALGSLLTGAAVAAVPAAGHAQMGTLPGPAPAGTPTW
                     AKGVEGQRKADLGNGTFLNPILAGDRPDPSILKDGDDYYMTHSSFDAYPGLLIWHSKD
                     LVNWAPVVASLTTNIGAVWAPEICKHKGRFYIYIPAKFPNNNTSYVIWADKIEGPWSE
                     PIDLKLPRYIDPGHIVDETGVRWLFLSGGDRIQLAPDGLSTVGKPEHVYDPWRYPDDW
                     DVEGFSPEGPKVMKKGDYYYMITAVGGTAGPPTGHMVIVARAKSLAGPWEDHPRNPLV
                     RTVDNTEKWWSRGHATLVEGPAGDWWTVYHGYENGFYTLGRQTLLAPVTWTADGWFDI
                     GGGDLAQPMAKPKGGKAGPHGMALSDDFSTDKYGVQWNFFDPKPGEKDRIKREGGVLT
                     LKGAGEAPSTGSPLIFVNGDQAYEIECEIDIDPDTRAGLILFYDRQLYCGLGFDAKNF
                     VTHQYGIERGRPANPHGSKMLMRLRNTRHIVSFHTSGDGGATWKRFDRGMEVSGYHHN
                     VRGGFLMLKPGLYAAGKGSARFRNFKYRALD"
     gene            2408..3253
                     /locus_tag="PLH31_06390"
     CDS             2408..3253
                     /locus_tag="PLH31_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HQR88883.1"
                     /translation="MATPALAQTAPEPAPFKSPRIVLIGDSTTAVQGGWGPSFCGKHV
                     TSAVACINLARGGRSSGNYRTEGSWALAMYEIRSGGFSDTYVLIQFGHNDQPGKPGRS
                     TDLVTEFPVNLRRYVDEVRAAGGKPVLVTPLTRRQFRDGKLVDDLGPWAQATRKVAAE
                     TGTPLVDLHALSTAAVQAMGPVEAMKFAQGAPDPRVREAARSGTTIEARFYEPAIPGA
                     TVPPAPAPTPPPVQNNAAAEPMGTPKQTFDYTHLGHEGADYFSALVATALADAVPALR
                     RQLLP"
     gene            complement(3310..>3425)
                     /locus_tag="PLH31_06395"
     CDS             complement(3310..>3425)
                     /locus_tag="PLH31_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQR88884.1"
                     /translation="LAKESAKLQSLQTKQQLGVQALSIANQSSSSILSLFR"
BASE COUNT          589 a         1176 c         1104 g          556 t
ORIGIN      
        1 gatcggctcg gctggcatgg ccgtgaccct gggcgtcctg acctggtgct tctcgaccgc
       61 caccacggtc aacggcgccc tgaccctcgg cgaccaggtc ggcctgaccg ccctgatcgc
      121 cgccaacctg tacgtggtgt tcttcaacct cagctggggc ccggtgatgt gggtcatgct
      181 gggcgagatg ttccccaacc agatgcgcgg ttcggccctg gccgttgcgg gcttcgccca
      241 gtggatcgcc aacttcctga tctcggtcag cttcccggcc ctggcggcca tcagcctgcc
      301 gatgacctac ggcttctatg cggtaagcgc cctggtctcg ttcttcctgg tccagaagct
      361 gatccaggaa acccgcggca aggaactgga agacatgaag ggctgacgat cctccccctg
      421 aagggggagg cggtccccaa cttgatcggg ggccggaggg ggaagtcggc aatgactgcg
      481 ccgatacttc ccccttccgt cggctgcgcc gacacctccc ccactggggg gaggacctta
      541 caagacgccc gggaggtttt gcctcccggg cgttttcgta tgtaatcagc cttcccaagg
      601 cggcgccccg gtgacagcgt tgtccctcga aaagcagaat gacaccggtg gacatcggtc
      661 gaaacttgcc gctaagtgac cggtaacacc gccgacgcga cgcagttcgc gcggtttgag
      721 tcacctggga aggaaacgaa aatggtccag atcagccgac gcggagccct gggctcgctt
      781 ctgaccggag ccgccgtcgc cgccgttccc gccgctggtc acgcccagat ggggaccctg
      841 cctggccccg cgcctgccgg aacgccgacc tgggccaagg gcgtcgaggg ccagcgcaag
      901 gccgacctgg ggaacgggac cttcctcaat ccgatcctgg ccggcgaccg gccggacccg
      961 tcgatcctga aggacggcga cgactactac atgacccact cgtcgttcga cgcctatccg
     1021 ggcctgctga tctggcattc gaaggatctg gtgaactggg cgccggtggt cgcctcgctg
     1081 acgaccaata tcggtgctgt ctgggcgccg gagatctgca agcacaaggg ccgcttctac
     1141 atctacatcc cggccaagtt cccgaacaac aacaccagct atgtgatctg ggccgacaag
     1201 atcgagggcc cctggtccga gccgatcgac ctgaagctgc cgcgctatat cgatcctggc
     1261 cacatcgtcg acgagaccgg cgtgcgctgg ctgttcctgt cgggcggcga ccgcatccag
     1321 ctggcgccgg atggcctgtc gaccgtgggc aagcccgagc acgtctatga tccttggcgc
     1381 tatcccgacg actgggacgt cgagggcttc tcgcccgagg gccccaaggt gatgaagaag
     1441 ggcgactact actacatgat caccgccgtg ggcggcacgg cgggcccgcc gaccggccac
     1501 atggtgatcg tggctcgcgc caaatcgctg gccggtccat gggaggacca tccgcgcaac
     1561 ccgctggtcc gcaccgtcga caacaccgaa aagtggtggt cgcgcggtca cgccaccctg
     1621 gtcgaaggcc cggccggcga ctggtggacc gtctatcacg gctatgaaaa cggcttctat
     1681 acgctgggcc gtcagaccct gctggcgccc gtcacctgga ccgcagacgg ctggttcgac
     1741 atcggcggcg gcgatctggc ccagccgatg gccaagccga aaggcggcaa ggccgggccc
     1801 cacggcatgg cgctgtcgga cgatttcagc actgacaagt atggcgtgca gtggaacttc
     1861 ttcgacccca agccgggcga gaaggaccgc atcaagcgcg aaggcggcgt gctgaccctg
     1921 aagggcgcgg gcgaggcgcc cagcaccggt tcgccgctga tcttcgtcaa tggcgaccag
     1981 gcctatgaga tcgaatgcga gatcgacatc gatcccgaca cccgagcagg cctgatcctg
     2041 ttctatgacc gccagctcta ttgcggcctg gggttcgacg ccaagaactt cgtcacccac
     2101 cagtacggca tcgagcgcgg ccgcccggcc aatccgcatg gctccaagat gctgatgcgc
     2161 ctgcgcaaca cccgccacat cgtcagcttc cacaccagcg gagacggcgg cgccacctgg
     2221 aagcgcttcg accggggcat ggaagtcagc ggctaccacc acaatgtgcg cggcgggttc
     2281 ctgatgctga agccgggcct ctatgcggcc ggcaagggct cggcgcggtt ccggaacttc
     2341 aagtaccggg cgctggattg aggggatcgc gatgcgtttg aaaccccttc tcctcgccgc
     2401 cgctctcatg gcgacgcctg ccctggccca aacggcgcct gaacccgccc ccttcaagtc
     2461 ccccaggatc gtgctgatcg gcgactccac caccgccgtg cagggcggct ggggccccag
     2521 cttttgcggc aagcacgtca cctcggccgt ggcctgcatc aacctggcgc ggggcggccg
     2581 cagcagcggc aattatcgca ccgagggctc gtgggccctg gcgatgtacg agatcaggtc
     2641 gggcggcttt tccgacacct atgtgctgat ccagtttggc cacaacgacc agccgggcaa
     2701 gccgggtcgc tccacggatc tcgtcaccga gttcccggtc aacctgaggc gctatgtcga
     2761 cgaggttcgc gccgccggcg gcaagccggt gctggtcacg cccctgacgc gtcggcagtt
     2821 cagggacggc aagctggtcg atgatctggg gccgtgggcg caggccaccc gcaaggtcgc
     2881 cgccgagacc gggacgccgc tggtcgacct gcacgcgctg agcaccgccg ccgtccaggc
     2941 catgggtccg gtggaggcga tgaagttcgc gcagggcgcg cccgatcccc gggtgcgcga
     3001 ggcggcaaga tccggcacca ccatcgaggc caggttctat gagccggcga tccccggcgc
     3061 gaccgttccg ccggcccccg cgccgacgcc tccgccagtc cagaacaatg cggcggccga
     3121 gccgatgggt acgcccaaac agaccttcga ctacacgcac ctgggccacg agggggctga
     3181 ctacttttcg gccttggtcg ccacggccct ggccgacgcg gtccctgccc tgcggcggca
     3241 actgctgccc tgaacgaaaa aagggcgggt ccgaagaccc gccctttccc tcttccgtaa
     3301 agccttggct taacggaaca ggctgaggat cgaagacgag gactggttgg cgatcgacag
     3361 cgcctgcaca cccagctgct gcttggtctg cagcgactgc agcttggcgc tttccttggc
     3421 caggt
//
LOCUS       DAOMEO010000225         3416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3134_length_3416_cov_2.444511, whole genome shotgun sequence.
ACCESSION   DAOMEO010000225 DAOMEO010000000
VERSION     DAOMEO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3134_length_3416_cov_2.444511"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..806
                     /locus_tag="PLH31_06400"
     CDS             81..806
                     /locus_tag="PLH31_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HQR88885.1"
                     /translation="MSIWRKLFTLGRAGAHEASAAVVDANAIRILDQEIRDADTAQGK
                     ARDDMATLVARRRILEKEVASFREQVIKYEGSARAAVTKGDMDLARQVAQRIADLEQD
                     IALKEPQIGDMRAAEEQLHQAITATDRRIETLRREVEVVKVNESVQKAQASVAARGAG
                     AGSSLGSAADSLARIKERQAIRGERIKAAGELEDRRTGADLDEKLRLAGIGPGQSSAD
                     DILASLAPPAAAPLQIEQKSSET"
     gene            812..>1767
                     /locus_tag="PLH31_06405"
     CDS             812..>1767
                     /locus_tag="PLH31_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HQR88886.1"
                     /translation="MTPRADWQAKADAVGFVYHHTDGQPYWDESAAYAFTLAQIEDDL
                     EPAAAELHELCLDLVDEAVGSEALMTRLAIPPEHQDFIAASWRAKEPPLYGRFDFGYD
                     GVGKPKLYEYNADTPTSVFEAATFQWLWLEELLASGALPTGTDQFNSLFEALRDRFST
                     VFPSGGFVHFASDAQAVEDRQTVRFLEDLALQAGLDPKFVAIDAIGVNGEGQFVDTEG
                     FIVQAAFKLYPWEFMLREPYAVHLKTAGVRWLEPPWKAILSNKGILPLLWERHPGHPN
                     LLEAYFEHDPRASALGPRYVRKPLFSREGANVELFGLGASHEP"
     assembly_gap    1768..1777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1778..1967
                     /locus_tag="PLH31_06410"
     CDS             <1778..1967
                     /locus_tag="PLH31_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HQR88887.1"
                     /translation="DQGYGQEGAIVQAYHPPPTFAGKHPVVGAWMVGNAPAGMGIRED
                     DSVVTKNLARFVPHIIEA"
     gene            2180..2458
                     /locus_tag="PLH31_06415"
     CDS             2180..2458
                     /locus_tag="PLH31_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88888.1"
                     /translation="MSIAAEACGKLDDALGQILAVSPPESRNQVMQELHTVDLLAQHI
                     TAVTDFTHRLALIASTDDSLAVDDALSAITLGAVAERLRASLAAAKAD"
     gene            2462..3034
                     /locus_tag="PLH31_06420"
     CDS             2462..3034
                     /locus_tag="PLH31_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQR88889.1"
                     /translation="MPRDSEQPSLFGFEAAPRDKHLILMIYPDQQATAEAQRRARRYQ
                     LDNGLGGVIFDPDLMHITLGHFDYWDDVPASLIERISRAASKLSMAPFEVSFDQIGGF
                     PKTVVLRGGEGLRALEAFQAALRQALAWEKLDGAHMNFTPHVTVVYGQPGAVVAAIEP
                     VTWTVKDFALVISHHGKGRHEVLARYPLVG"
     gene            3091..>3416
                     /locus_tag="PLH31_06425"
     CDS             3091..>3416
                     /locus_tag="PLH31_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HQR88890.1"
                     /translation="MSVTIYGIKACDTMNKARDWLGGQGVSYAFHDYKAAGIARARLE
                     QWVEVLGWETVLNRAGTTFRKLADADRQALDAGKAIDLMLAQPSMIKRPILDIDGKLI
                     AGFKPE"
BASE COUNT          609 a         1118 c         1118 g          561 t
ORIGIN      
        1 agatcgggca tgctgaaggc agcaggatcg tcgtcctaat aaaattaaac agagcctgat
       61 ttcgatttcg gagacctctc atgtccatct ggcgcaagct gttcaccctg ggccgcgcgg
      121 gcgcgcatga agcctcggcc gccgtcgtcg acgccaacgc catccggatc ctggaccagg
      181 aaatccgcga cgcggacacg gctcagggca aggcccgcga cgacatggcc accctggtgg
      241 cccgtcgccg gatcctggaa aaggaagtcg ccagctttcg cgagcaggtg atcaagtacg
      301 agggctcggc ccgcgccgcc gtcaccaagg gcgacatgga cctggcccgc caggtggccc
      361 agcgcatcgc cgatctcgaa caggacatcg cgctgaagga gccccagatc ggcgacatgc
      421 gcgccgccga ggagcaactg catcaggcca tcaccgccac cgaccgccgc atcgagaccc
      481 tgcgccgcga agtcgaggtc gtgaaggtca atgagtcggt ccagaaggcc caggcctcgg
      541 ttgcggcgcg cggcgctggc gccggctcgt cgctcggctc ggccgccgat agcctggccc
      601 gcatcaagga acgccaagcc atccgcggcg agcgcatcaa ggccgccggc gaactggaag
      661 accgtcgtac gggcgccgat ctggacgaga agctgcgctt ggccggcatc ggccccggcc
      721 agtcgtcggc cgacgacatc ctggccagtc tcgccccgcc ggccgccgcc ccgctgcaga
      781 tcgagcagaa gtcgagcgaa acctagagcg catgacgccg cgcgccgact ggcaggcaaa
      841 ggccgacgcc gtcggcttcg tctatcacca caccgacggc cagccctatt gggacgagtc
      901 tgcggcctat gccttcaccc tggcccagat cgaggacgat ctggagccgg cggcggcgga
      961 gctgcatgag ctttgcctgg acctggtcga tgaggcggtc ggcagtgaag ccctgatgac
     1021 ccggctggcg atcccgcccg agcaccagga cttcatcgcc gcgtcctggc gcgccaagga
     1081 gccgccgctc tatggccgct tcgacttcgg ctatgacggc gtcggcaagc ccaagctcta
     1141 cgaatacaac gccgacacgc cgaccagcgt gttcgaagcg gcgaccttcc agtggctatg
     1201 gctggaagag ttgctcgcct ctggcgcctt gccgactggg actgaccagt tcaacagcct
     1261 gttcgaagcc ctacgtgacc ggttcagcac cgtgttcccg agcggcggtt tcgtgcactt
     1321 cgccagcgac gcccaggccg tcgaggatcg ccagaccgtg cgcttccttg aggacctggc
     1381 gcttcaggcc ggtctggacc cgaagttcgt cgccatcgac gcgatcggcg tcaacggcga
     1441 gggacagttt gtcgataccg agggcttcat cgtccaggcg gcgttcaagc tctatccatg
     1501 ggagttcatg ctgcgcgaac cctatgccgt ccacctgaag acggcgggcg tgcgctggct
     1561 ggagccgcct tggaaggcga tcctgtccaa caagggtatc ctgcccctgc tttgggagcg
     1621 tcacccgggg catcccaacc tgctggaagc ctatttcgag cacgacccgc gcgcttcggc
     1681 gctggggcca cgttatgtgc gcaagccgct gttctcgcgg gaaggcgcca atgtcgagct
     1741 ctttggcctc ggtgcgagcc acgagccnnn nnnnnnngga tcagggctat ggccaggaag
     1801 gcgcgatcgt gcaggcctat cacccgccac cgacctttgc cggcaagcat cctgtggtgg
     1861 gggcctggat ggtcggcaac gcgccggctg gcatgggcat ccgcgaggac gacagcgtgg
     1921 tcaccaagaa cctcgcgcgc tttgttccgc atatcatcga ggcttgaggc gaccccggat
     1981 cggggggctc aattcgcctg acgggcgtaa tcgcgcttgc ggtccgaccc gggatcactc
     2041 caacgtgacg caacggccgc gtgcgcgatg cgcccaggct tgaaaacaca cagagaccaa
     2101 cgtcggggga atgatgactt cggaacagga cagcgcgggt tcgagcatag ccgcgatttt
     2161 ggcggccgtt tcggcggaga tgtcgatcgc tgccgaggcc tgcggaaagc tggacgacgc
     2221 gctggggcag atcctggcgg tcagcccgcc ggaaagccgc aaccaggtga tgcaggagtt
     2281 gcataccgtg gacctgctgg cccagcacat caccgccgta accgacttca cccatcgact
     2341 ggccttgatc gcctcgacgg acgactctct ggcggtcgat gacgcgctgt cggccatcac
     2401 cctgggggct gtggccgaac ggctgcgggc cagtctggcc gcagcgaagg cagactgatc
     2461 catgccgagg gattccgagc agccctcgct gtttggcttc gaggccgcgc cgcgcgacaa
     2521 gcatctgatc ctgatgatct atccagatca gcaggcgacg gctgaagccc agcggcgagc
     2581 ccggcgctat cagctcgata acgggctggg cggcgtcatc ttcgatcccg acctgatgca
     2641 catcacgctc ggccactttg actactggga cgacgtgccg gcgagcctga tcgagcggat
     2701 ttctcgcgcg gcgtccaaac tatccatggc gccgttcgag gtgtcgttcg accagatcgg
     2761 cggtttcccc aagaccgtgg tgctacgcgg cggcgagggg ctgagggcgc tcgaagcgtt
     2821 ccaagccgcg cttcgccagg ccttggcctg ggagaagctc gacggcgcgc acatgaattt
     2881 cacgcctcac gtgacggtcg tgtatggcca gcccggcgcg gtggtcgcgg ccatcgagcc
     2941 cgtcacctgg accgtgaagg acttcgctct ggttatcagc caccacggca aggggcggca
     3001 tgaagtgttg gctcgctatc cgctggtcgg ttgacttgcg cccatgggcg ggcacggcta
     3061 cccgtcgccg tcttcaggaa aggactggcg atgagcgtca cgatctacgg catcaaggcc
     3121 tgtgacacga tgaataaggc ccgtgactgg ttgggcgggc agggcgtgtc ttacgctttt
     3181 cacgactaca aggccgccgg gatcgcccgc gcccgcctgg agcagtgggt cgaagtcctg
     3241 ggttgggaga cggtgctcaa tcgcgcgggc acaaccttcc gcaaactggc cgacgccgac
     3301 aggcaggcgc ttgacgctgg caaggccatc gacctgatgc tggcacagcc ttcgatgatc
     3361 aagcgcccca ttctcgacat cgatggcaaa ctgatcgcgg gcttcaagcc tgaaat
//
LOCUS       DAOMEO010000226         3413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3138_length_3413_cov_2.189696, whole genome shotgun sequence.
ACCESSION   DAOMEO010000226 DAOMEO010000000
VERSION     DAOMEO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3138_length_3413_cov_2.189696"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLH31_06430"
     CDS             <1..802
                     /locus_tag="PLH31_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR88891.1"
                     /translation="VGSLTSSFEDARLRQILNNLLSNAVKFTERGVIDLRLAVEAVGD
                     GMLRVVVEVQDNGPGITADTMARLFTPYTQASQVTARTYGGTGLGLAVSRQLARLMGG
                     ELDARPGTSGGAVFRLTLTLAAGREAAIASAPALEGILPTISTLKVLAVDDHEVNRRT
                     LALVLEPLGVALSTATDGLAALQCLAAERFDIVLMDVNMPGLDGREATRQLRAGDGVN
                     RDIPVIGFSAGVAAEEVQACRDAGMTDWLAKPLDIRALYDALDRAVAA"
     gene            complement(808..1968)
                     /locus_tag="PLH31_06435"
     CDS             complement(808..1968)
                     /locus_tag="PLH31_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQR88892.1"
                     /translation="MSMPFIDLAAQQRRIRDRIDVAMAAVLDSGAYVMGPQVREFESR
                     LAAFGQARLALSCANGTDAIALPLMAWGVGKGDAVFCPSFTFAATPEVVPWVDATPVF
                     VDILPDTFNLDPVKLEAAIAGVKAEGRLTPKVVIAVDLFGQPADYPAIKAICDREGLK
                     LIADSAQGFGCTLDGKHPLHWADVATTSFFPAKPLGCYGDGGAVLTNDQDVWDLMDSF
                     RVHGKAVAPDLVGRTFDHDTKYLNTRIGMNSRLDTLQAAILIEKLAIFAEEIELRQVV
                     ADRYAEGLQGAVLATPKVIDGGVSVWAQYVIEHENRDGLAAHLKTQGIPTAVYYPVPM
                     HIQAPYADFPRGVGGLPVTEAKAETVLALPMHPYLEAADQQKIIDAIRAFNG"
     gene            2115..3098
                     /locus_tag="PLH31_06440"
     CDS             2115..3098
                     /locus_tag="PLH31_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQR88893.1"
                     /translation="MTKPTLIPTAGKLPDLANMKADETLLTHVFDWAGKVAVNLVVAA
                     LILGLTFWIAGWAAGVVRRTLGRVHRNNPDHTLETFGASLVRYAIVVVGLVAVLQQLG
                     VQATSIIAVLGAASLAIGLALQGALSNVAAGVMILLFRPYRVGDTIETVTRIGTVKSL
                     DLLFTEIATFDNVKVMIPNSKVFGDVILNYSAHRTRRVDVLFKVPLKTDLVAVLKRLR
                     ERAENDPRIRKEPAVQIEVTDLSEAFAQAAIRAWTAREDFASVKTDLMLTAHLLADDP
                     VREDLPPPRPTKAIDPSPAMPGEGEHHLFKLPRRRARQPGPSKSPSQPKKA"
     assembly_gap    3174..3183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3184..3398)
                     /locus_tag="PLH31_06445"
     CDS             complement(<3184..3398)
                     /locus_tag="PLH31_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88894.1"
                     /translation="MVPTDATAEIRFADPDEAASFSTFVQGFLSANGFPFVIIHDAPE
                     VVGHMRRVVFEDAGISRKFAQEWVNLRG"
BASE COUNT          555 a         1136 c         1146 g          566 t
ORIGIN      
        1 cgtcggttcc ctgaccagct cgttcgagga cgcccgcctg cgccagatcc tcaacaacct
       61 gctgtccaac gccgtgaagt tcaccgagcg cggggtgatc gacctgcggc tggcggtcga
      121 ggccgtcggc gacgggatgc tgcgggtcgt ggtcgaggtg caggacaacg gccccgggat
      181 caccgccgac accatggccc gactgttcac gccctatacc caggcctcgc aggtgacagc
      241 gcgcacctat ggcggtacgg gcctgggcct ggccgtcagc cgtcagttgg cgcgcctgat
      301 gggcggcgag ctggacgccc ggcccgggac ctctggcggc gcggttttcc gcctgaccct
      361 gaccctcgcg gcgggccgag aggcggcgat cgccagcgcc ccggcccttg agggtatcct
      421 tccgaccatt tcgacgttga aggtcctggc ggtcgacgac catgaggtga accgtcgaac
      481 cctcgccctg gtcctcgagc cgctgggcgt ggccctgtcg accgcgaccg atggcctggc
      541 ggcgctacaa tgcctggccg ccgagcgctt cgatatcgtg ctgatggacg tcaacatgcc
      601 cgggctggat gggcgcgaag cgacccgcca actgcgggcc ggagacgggg tcaaccgtga
      661 tatcccggtg atcggcttca gcgccggggt ggcggccgag gaggtccagg cctgtcgtga
      721 cgccggcatg acggactggc tggccaagcc cctggacatc cgcgccctct atgatgcgct
      781 ggaccgcgcc gtcgccgcct gaagccctca gccgttgaag gcgcggatcg cgtcgatgat
      841 tttctgttgg tcggcggctt ccagataggg atgcatcggc agggccagca cggtctcggc
      901 cttggcctcc gtcacgggca ggcctccaac gccgcgcggg aagtccgcat agggcgcctg
      961 gatgtgcatc ggcaccggat aatagaccgc cgtcgggatg ccctgggtct tcagatgggc
     1021 ggccaggcca tcgcggttct cgtgctcgat cacatactgg gcccagaccg agacgccgcc
     1081 gtcgatgacc ttcggtgtcg ccagcacggc gccctgcagg ccctcggcat agcgatcggc
     1141 caccacctgc cgcagctcga tctcttcggc gaagatggcc agcttctcga tcaggatcgc
     1201 cgcctggagg gtgtcgaggc gcgagttcat gccgatgcgg gtgttcaggt acttggtgtc
     1261 gtggtcgaag gtgcgaccga ccaggtctgg ggcgaccgcc ttgccgtgga cgcggaagct
     1321 gtccatcagg tcccatacgt cctggtcgtt ggtcagcacc gcgccgccgt cgccgtagca
     1381 gcccagcggc ttggccggga agaagctggt ggtggccacg tcggcccagt gcagcgggtg
     1441 cttgccgtcg agggtgcagc caaagccctg ggcgctgtcg gcgatcagct tcaggccttc
     1501 gcggtcacag atggccttga tggccggata gtcggcgggc tggccgaaca gatccaccgc
     1561 gatcaccacc ttgggcgtca gcctgccctc ggctttcacc ccggcgatgg cggcttccag
     1621 cttgacgggg tcgaggttga aggtgtcggg caggatatcg acaaacaccg gcgtcgcatc
     1681 gacccacggc acgacttccg gcgtcgcggc gaaggtgaag gacgggcaga acaccgcgtc
     1741 gcccttgccc acgccccagg ccatcagcgg cagggcgatg gcgtcagtgc cgttggcgca
     1801 ggacagggcc agtctggcct ggccgaacgc ggccagccta gactcgaact cgcgcacctg
     1861 cggacccatc acataggccc cgctgtcgag gacggcggcc atagccacgt cgatcctgtc
     1921 gcggatccgg cgctgctgcg cggcgaggtc gatgaagggc atgctcatgg gcgcggctct
     1981 taagggcgga tcgactcgaa ttcgagccaa ttgatgtaac cgactgtttc gaaaggtcga
     2041 agagcagctt ttacgggagc agaggcaagc gccggtcgtt gaccgccaat ggcgggggcg
     2101 ccatcgaaag gtccatgacc aagccgaccc tgattccgac cgccggcaag ctgcccgacc
     2161 tcgccaatat gaaggccgac gagaccctgc tgacccacgt cttcgactgg gctggcaagg
     2221 tggcggtcaa tctggtggtc gccgccctta tcctgggcct gaccttctgg atcgcgggct
     2281 gggcggccgg ggtggtgcgg cgaaccctgg gccgcgtcca tcgcaacaat cccgaccaca
     2341 ccctggaaac cttcggggcc tccctggtcc gctacgccat cgtggtggtc ggtctggtgg
     2401 ccgtgctgca acagttgggc gtgcaggcga catcgatcat cgctgtgctg ggcgcggcct
     2461 cgctggccat tggcctggct ctgcagggcg ctctctcgaa cgtggccgcc ggcgtgatga
     2521 tcctgctgtt ccggccctac cgcgttggcg acaccatcga aacggtcacc cgcatcggca
     2581 cggtcaagtc gctggacctg ctgttcaccg agatcgccac cttcgacaac gtcaaggtga
     2641 tgatccccaa cagcaaggtg ttcggcgatg tgatcctcaa ctattcggcc caccgcaccc
     2701 gccgcgtgga cgtgctgttc aaggttcccc tgaagaccga tctggttgcg gtgctgaaac
     2761 gcctgcgcga gcgggccgag aacgatcctc gcatccgcaa ggagccggcc gtgcagatcg
     2821 aggttaccga cctgtcggaa gcctttgccc aggccgcgat ccgcgcctgg acggccaggg
     2881 aagacttcgc ctcggtcaag accgacctga tgttgaccgc ccatctgctg gccgacgacc
     2941 cggtccgcga ggatctcccg ccaccgcgcc cgaccaaggc catagacccc tcaccggcca
     3001 tgccgggcga gggtgagcac catctgttca agctgccaag gcgtagagct cggcaaccag
     3061 gtcccagcaa gtctccgtcc caaccgaaaa aagcctgacc ccgatctcag ggcccgcacg
     3121 tccggccctg ggcggcgggt gagggcgcct ggaggaagcg cgcctgtaga accnnnnnnn
     3181 nnnccgcgca ggttgaccca ctcctgagcg aactttcggc tgatcccggc gtcctcgaac
     3241 accacgcggc gcatatggcc gacgacctcg ggggcgtcat gaatgatcac gaacggaaag
     3301 ccgttggccg agagaaagcc ctgtacgaag gtcgagaagc tggcggcctc atcagggtct
     3361 gcgaagcgta tctcggcggt ggcgtcggtc gggaccatgg tggtctcccc tat
//
LOCUS       DAOMEO010000227         3402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3151_length_3402_cov_2.371377, whole genome shotgun sequence.
ACCESSION   DAOMEO010000227 DAOMEO010000000
VERSION     DAOMEO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_3402_cov_2.371377"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2496
                     /gene="gyrA"
                     /locus_tag="PLH31_06450"
     CDS             <1..2496
                     /gene="gyrA"
                     /locus_tag="PLH31_06450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQR88895.1"
                     /translation="HTPDRAYVKSARVVGDVMGKYHPHGDASIYFTLVRMTQPFSMGL
                     ILIDGQGNFGSVDGDMPAAMRYTECRMAPPALALLADLDKDTVDFQDNYDGKEQEPTV
                     LPSRIPNLLVNGAGGIAVGMATNIPPHNLGEIIDACLAMVDDPDVSTETLLDIVPGPD
                     FPTGGEIIGRAGPRQALLTGRGSVIMRGLATVEEIRQGREAIIITAIPYQVNKAHLVE
                     HIAELVRDKKIEGVSDIRDESNRDGMRILVELKRDASGEVILNQLYRFTALQSSFGVN
                     MLALNRKRPEQMGLRQLVEIFVKFREEVVVRRTKFELGKARDRGHVLVGLTIAVANID
                     EFIHIIRSSKDPAEARERLVAKAWPAGDMLPLVELIADPRSLHEEGGLIRLTDEQARA
                     ILALTLSRLTGLGREEIGNEAQALAEAIRGYLELLSDRANIMAVVREELVEVREKFAV
                     PRRCQIVDGDADVEDEDLIVREEMVITVTMGGYVKRTPLANYRTQHRGGKGKSGMATK
                     TEDAVTRVFSASTHAPLLFFTSGGKVYKLKVWRLPLGAANSRGKAFVNLLPIEPGETI
                     TSILTLPEDESTWDRLDVMFATRSGSVRRNKLSDFVDVRRNGKIAMKLDDGDGIVGVG
                     VCGTENDILLTTSVGRCIRFSADEVRVFASRDSTGVRGVKLAEGDSVINMAILRGVDA
                     TPAERTAYLKHQRALAKARGEDGEDVAAPDEPEEDVGDAALSLERISAYDYRRTGRGG
                     QGLTAQDLSKRGGRLAASFPVEDSDEILLVTDAGQLIRCPVAQIRVAARNTQGVTVFR
                     TAKDEHVVSVERLADAGGDDSDQGEPSGADETL"
     assembly_gap    2550..2559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2560..3011
                     /gene="coaD"
                     /locus_tag="PLH31_06455"
     CDS             <2560..3011
                     /gene="coaD"
                     /locus_tag="PLH31_06455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQR88896.1"
                     /translation="NGHLDIIGRAVKLVDKLVIGVAVNIGKGPLFSLEERVEILRNET
                     AHLSQVAVIEVKPFDSLLMHFAREVGAQMIVRGLRAVADFEYEFQMTAMNQQLDREIE
                     TVFLMADPRHQAIASRLVKEIAALGGDISKFVPAGVGEQLVSKLRKA"
     gene            3124..>3402
                     /locus_tag="PLH31_06460"
     CDS             3124..>3402
                     /locus_tag="PLH31_06460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR88897.1"
                     /translation="MKLAYSVAALALLAATAPLQAAMAQTSSDWRTPDPQNVLVVETN
                     KGRIIAELSPLAAPAHVARIRELVQANFYDGLTFFRVIDSFMAQTGDPE"
BASE COUNT          600 a         1091 c         1138 g          563 t
ORIGIN      
        1 catacgccgg atcgcgccta tgtgaagtcg gcacgcgtgg tcggtgacgt gatgggtaag
       61 tatcacccgc acggcgacgc ctcgatctac ttcaccctgg tgcggatgac ccagccgttc
      121 tcgatgggtc tgatcctgat cgacggccag ggcaatttcg gctcggtcga cggcgatatg
      181 cccgcggcca tgcgatatac tgagtgccgc atggcgcccc cggcgctggc cctgctggcc
      241 gatctggaca aggacacggt cgacttccag gacaattacg acggcaagga gcaggaaccg
      301 acggtcctgc cgtcgcggat tccgaacctg ctggtcaatg gcgcgggcgg catcgccgtc
      361 ggcatggcca ccaacatccc gccgcacaat ctgggcgaaa tcatcgacgc ctgtctggcc
      421 atggtcgacg accctgacgt cagcaccgag accctgctgg atatcgttcc gggtcccgac
      481 ttcccgaccg gcggcgagat catcggccgg gctgggcccc gtcaggcgct gctgaccggg
      541 cgcggctcgg tgatcatgcg cggtctggcg acggtggaag agatccgcca gggccgcgaa
      601 gccatcatca tcaccgccat cccgtatcag gtgaacaagg cccacctggt cgagcacatc
      661 gccgaactgg tgcgcgacaa gaagatcgag ggcgtcagcg acattcgcga cgagtcgaac
      721 cgcgacggca tgcgcatcct ggtcgagctg aagcgcgacg cctcgggcga ggtgatcctc
      781 aaccagctgt accgcttcac ggccctgcaa agctcgttcg gcgtcaacat gctggccctg
      841 aaccgcaagc gccccgagca gatgggtctg cgccagctgg tcgagatctt cgtcaagttc
      901 cgcgaagagg tggtcgtccg gcggaccaag ttcgagctgg gcaaggcgcg cgatcgtggc
      961 cacgtgctgg tgggcctgac catcgctgtg gccaatatcg atgagttcat ccacatcatc
     1021 cgctcgtcca aggatccggc cgaggcccgc gagcgcctgg tcgccaaggc ctggccggct
     1081 ggcgacatgc tgccgctggt cgagctgatc gccgatccgc gcagcctgca cgaagagggc
     1141 ggcctgatcc gcctgaccga cgagcaggcc cgcgccatcc tggccctgac cctgtcgcgt
     1201 ctgacgggcc tgggccgcga ggaaatcggc aatgaggcgc aggctctggc cgaggccatt
     1261 cgtggctatc tggaactgct gtcggaccgc gccaacatca tggccgtggt tcgcgaagaa
     1321 ctggtcgagg tgcgcgagaa attcgccgtg ccgcgccgct gccagatcgt cgacggcgac
     1381 gccgacgtgg aagacgagga cctgatcgtc cgcgaggaaa tggtcatcac cgtgaccatg
     1441 ggcggctatg tgaagcgcac gccgctggcc aattaccgca cccagcaccg gggcggcaaa
     1501 ggcaagtcgg gcatggcgac caagaccgag gatgcggtca cccgcgtgtt ctcggcctcg
     1561 acccacgcgc cgctgttgtt cttcacctcg ggtggcaagg tctacaagct gaaggtctgg
     1621 aggctgccgc tgggcgccgc caattcgcgc ggcaaggcgt tcgtcaacct gctgccgatc
     1681 gagccgggcg agaccatcac ctcgatcctg accctgcccg aggacgagag cacctgggac
     1741 cgcctggacg tgatgttcgc cacccgctcg ggcagcgtgc gccgcaacaa gctgagcgac
     1801 ttcgtggatg tgcgccgcaa cggcaagatc gccatgaagc tggatgacgg cgacggcatt
     1861 gtcggcgtcg gcgtgtgcgg aaccgagaac gacatcctgc tgaccacctc ggtgggccgc
     1921 tgcatccgct tctcggccga cgaggtgcgg gtgttcgcca gccgcgattc gaccggcgtg
     1981 cgcggcgtca agctggccga gggcgacagc gtcatcaaca tggcgatcct gcgcggcgtc
     2041 gacgccacgc cggccgagcg cacggcttat ctcaagcatc agcgtgcctt ggctaaagct
     2101 cggggtgagg acggcgagga cgtcgccgcg cccgatgagc ctgaagaaga tgtcggcgat
     2161 gccgcattgt ccttggaacg catcagcgcc tacgactatc gccgcaccgg ccggggcggg
     2221 caggggctta cggcccagga cctctccaag cgcggcggcc gcctggcggc gtcgttcccg
     2281 gtcgaggaca gcgacgaaat cctgttggtg acagacgccg gacaattgat acggtgcccc
     2341 gtcgcgcaaa ttcgtgttgc agcgcggaat actcagggcg taaccgtctt ccgcaccgcg
     2401 aaggacgagc atgtcgtgag tgttgagcgg ctggccgacg ccggcggcga cgacagtgat
     2461 caaggcgagc caagcggggc ggacgagacc ctgtaggccg ccgattaagc agggggtttc
     2521 atgcgggttg ggctttatcc cggaacgttn nnnnnnnnnc caatggccat ctcgacatca
     2581 tcggtcgggc cgtcaagctg gtcgacaagc tggtgatcgg ggtggcggtg aacatcggca
     2641 aagggccgct gttcagcctg gaagagcggg tcgagatcct caggaacgaa accgcccacc
     2701 tgtcgcaggt ggcggtgatc gaggtgaaac ccttcgacag cctgctgatg cacttcgccc
     2761 gcgaggtcgg agcccagatg atcgtgcgcg gcctgcgagc cgtggccgac ttcgaatacg
     2821 agttccagat gacggccatg aaccagcagc tggatcgcga gatcgagacg gtgttcctga
     2881 tggccgaccc gcgtcaccag gccatcgcct cgcggctggt caaggagatc gcggccctgg
     2941 gcggcgacat ctccaagttc gtgcccgccg gggtgggcga acagctggta tccaagcttc
     3001 gcaaggcctg atggcgcggc attgccgtga agctgtcgaa atcataggct tcaacatggc
     3061 ggacttgtcg cgggggcgac gtggctctac gccccgcatt cgcctttctg ttcggggatt
     3121 cccatgaagc ttgcgtattc cgtcgccgcc ctggcgctgc tggccgcgac cgcccctctt
     3181 caggcggcca tggctcagac gtcttctgac tggcgcacgc cggatcccca gaacgtgctg
     3241 gtggtcgaga ccaacaaggg ccggatcatc gccgagctca gcccgctggc cgcgccggcg
     3301 catgtggcgc gcattcgcga gctggttcag gccaatttct atgacggcct gaccttcttc
     3361 cgcgtcatcg acagcttcat ggcccagacg ggcgatccgg ag
//
LOCUS       DAOMEO010000228         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3165_length_3386_cov_2.721405, whole genome shotgun sequence.
ACCESSION   DAOMEO010000228 DAOMEO010000000
VERSION     DAOMEO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_3386_cov_2.721405"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLH31_06465"
     CDS             <1..1115
                     /locus_tag="PLH31_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR88898.1"
                     /translation="VYGRAPGDIIAAGAPIADLFNPDWAGAQTEYLAVRRIGDPRLTA
                     AARSRLTLLGMSQSLIAAVEHSGKVQTTSTVRAPIGGVIQTLDVRPGMSLAAGQSLAQ
                     ISGLATVWLTLSVPETQAGQIMIGQLVSARLPAFPGESFSGRVSAILPATQADSRTLQ
                     VRVELADRGGRLRPGMFAAASVGGEVAPVLTVPTEAVIRTGRRDLVMLAQAGGRYQPA
                     EVRVGRQAGDRTEILAGLSAGDQVVASGQFLLDSEASLAGLVPRSLTASTPVRSMPVA
                     APSGGAAQTVKRPTNAASSLLQAQGRIEEITADTITLSHGPVPAINWPAMTMTFKLDP
                     PSLARGLKAGDKAAFGFEQRGDGPTVRSLRRMEASR"
     gene            1112..>2720
                     /locus_tag="PLH31_06470"
     CDS             1112..>2720
                     /locus_tag="PLH31_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQR88899.1"
                     /translation="MIAAVIRWSLANRFFVLLGAMVLLASGLVALRETPLDALPDLSD
                     VQVVIRTPAQGQAPRLVENQITYPLATTMLSVPGAKTVRGYSFFGDSFVYVLFADGTD
                     PYWARSRVLESLSQVQSRLPAEARPALGPDATGVGWIFEYALIDRTGRHDLSQLRSLQ
                     DWFLRYELKTLPGVAEVASIGGMVRQYQVVLDPQKLAAYGVTHQAVVEALNGANQEAG
                     GSVVEMAEAEYMVRASGYLSSLDDFAAVPIKTAAGGVPITLGQVATIQIGPEMRRGIA
                     ELNGQGEVAGGVVVLRSGEDARATLKAVQDKLKILKTTLPPGVEIVTTYDRSGLIDRA
                     IDNLKSKLFEEFIVVALVCALFLWHLRSALVAIIALPLGVLAAFIVMRAQGVNANIMS
                     LGGIAIAVGAMVDAAVVMIENAHKHLERWTHDHPGQTIGEKERWDEIAAAAVEVGPAL
                     FLSLLIITFSFIPVFTLQGQEGRLFAPLAFTKTYAMAAAALLSITLIPVLMGYLIRGK
                     IPAETANPINRLLSAIYAPALDWVLGRP"
     assembly_gap    2721..2730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2731..>3386
                     /locus_tag="PLH31_06475"
     CDS             <2731..>3386
                     /locus_tag="PLH31_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR88900.1"
                     /translation="IAALVFATTLIPLSRLGGEFMPTLNEGDLLYMPSALPGLSAAKA
                     GALLQQTDRLIKTVPEVDSVFGKAGRAESATDPAPLEMFETTIRFKPRDQWRPGMTPE
                     KLVEELDRTVAVPGLSNVWVPPIRNRIDMLATGIKSPIGVKVAGANLDDLDRVAQQIA
                     QVAKTVPGVSSALAERLTGGRYVDVRIDRAAAGRYGLNIADVQAIVSGAIGGETVGET
                     "
BASE COUNT          532 a         1173 c         1107 g          564 t
ORIGIN      
        1 gcgtctatgg ccgcgcgccc ggcgacataa tcgcggctgg cgcgcctatc gctgacctgt
       61 tcaatcctga ttgggcgggt gcgcagaccg aatatctggc cgtgcgccgc atcggcgacc
      121 cgcgcctgac ggccgccgcc cggtcgcgcc tgactttgct tggcatgagc caatccctga
      181 tcgctgcggt cgagcacagc ggcaaggtcc agacgacgtc cacggttcgc gcgccgatcg
      241 gtggggtgat ccagaccctg gatgtcaggc ccggcatgag cctggccgcg ggccagagcc
      301 tggcgcagat cagcggtctt gcgaccgttt ggctgaccct gtcggtgcct gagacccagg
      361 ccgggcagat catgatcggc cagttggtat cggcccggct gcccgcgttt cctggcgaga
      421 gcttcagcgg ccgtgtcagc gccatccttc cagccaccca ggccgacagc cgtaccctgc
      481 aggtccgggt cgagctggcc gatcgcggcg gcaggctgcg cccgggcatg ttcgccgccg
      541 ccagtgtcgg gggcgaggtc gcgcctgtct tgaccgtccc caccgaagcg gtgatccgta
      601 ccgggcggcg cgacctggtg atgctggccc aggccggagg ccgctaccag cccgccgagg
      661 tgcgagtcgg tcgccaggcc ggcgatcgca ccgagatcct ggcaggcctt agcgcaggcg
      721 atcaggtcgt ggcctccggc caattcctgc tcgattccga agccagtttg gcgggcctgg
      781 tgcctcggtc cctcacggcg agcacgccag tgcggtcgat gcccgttgcc gcccccagcg
      841 gcggagccgc gcaaaccgtg aaacgcccga cgaatgcggc cagctcactc ctgcaggcgc
      901 agggtcggat cgaggagatc accgccgaca cgatcacgct cagccacggc ccggtgcccg
      961 cgatcaactg gccggccatg acgatgacct tcaagctcga tccaccaagt ctggcccgag
     1021 gattgaaggc gggcgataag gcagcgttcg gcttcgaaca acgtggggat ggacccacgg
     1081 tgcgcagtct ccgccgcatg gaggcaagcc gttgattgcc gccgtcatcc gctggtcgct
     1141 ggccaaccgc ttcttcgtac tgctcggggc catggtcctg ctggccagcg ggcttgtggc
     1201 gctgcgcgaa acaccactgg acgccctacc cgatctttcc gacgtgcagg tggtgatccg
     1261 cacgccagcc cagggccagg ccccgcgtct ggtcgagaac cagatcacct atcccttggc
     1321 caccaccatg ttgtcggtgc cgggcgccaa gaccgtgcgc ggctactcgt tcttcggcga
     1381 cagcttcgtc tatgtgctgt tcgccgacgg aaccgatccc tattgggccc gctcgcgggt
     1441 gctggagtcg ctgagccaag tccaaagccg gcttccggcc gaagcccgtc cggccctcgg
     1501 tcccgacgcc acgggtgtgg gctggatctt cgaatacgcc ctgatcgacc gaaccggccg
     1561 ccatgacctc agccagcttc ggtccttgca ggattggttc ttgcgctatg agttgaagac
     1621 cctgccgggg gtggccgagg tggccagcat cggcggcatg gtgcgccagt atcaggttgt
     1681 gctcgacccg cagaaattgg cggcctacgg cgtcacccac caagccgtcg tcgaggcgct
     1741 gaacggcgcc aaccaggaag ccggcggctc ggtcgtcgag atggccgaag ccgaatacat
     1801 ggtccgggcc agcggctacc tgagcagcct ggacgacttc gccgcggtgc cgatcaagac
     1861 tgcggccggc ggcgtgccca tcaccctggg ccaggtcgcc acaatccaga tcggcccgga
     1921 gatgcgccga gggatcgccg agctcaacgg ccagggcgag gtcgccggcg gggtggtggt
     1981 gttgcgctcg ggcgaggacg cccgcgccac cctcaaggcc gtgcaggaca agctgaagat
     2041 cctcaagacc accctgccgc caggcgtgga gatcgtcacc acctatgacc gctccggctt
     2101 gatcgaccgc gccatcgaca acctaaagtc caagctcttc gaggagttta tcgtcgtcgc
     2161 cctggtctgc gcgctcttcc tttggcatct gcgctcggcc ctggtggcaa tcatcgccct
     2221 gccgctgggc gtgctggccg ccttcatcgt catgcgcgcc cagggggtca acgccaacat
     2281 catgtcgcta ggcggcatcg ctatcgctgt cggggccatg gtcgatgcgg ccgtcgtgat
     2341 gatcgagaat gcgcacaaac atctcgagcg ctggacgcac gatcatcccg gccagacgat
     2401 cggcgagaag gaacgctggg acgagatcgc cgccgccgcc gtcgaggtcg gcccggccct
     2461 gttcctgagc ctgctgatca tcaccttctc gttcatccct gtgttcactc tgcagggcca
     2521 ggaggggcga ctctttgccc cgctggcctt caccaagacc tacgccatgg ccgcggcggc
     2581 cctgctgtcg atcaccctga tcccggtgct gatgggttat ctgatccgcg gcaagatccc
     2641 ggccgagacc gccaacccca tcaaccggct actgagcgcg atctatgcgc cggccttgga
     2701 ctgggtgctc gggcggccgc nnnnnnnnnn cattgccgcc ctggtcttcg ccaccacact
     2761 gatcccgctg tcgcggctgg gcggcgagtt catgccgacc ctgaacgaag gcgacctgct
     2821 ctacatgccc tcggcccttc cgggcttgtc ggcggccaag gccggcgcgc tccttcaaca
     2881 gaccgatcgg ctgatcaaga ccgtgcccga ggtcgacagc gtgttcggaa aggccggccg
     2941 cgcggaatcg gcgaccgatc cggccccgtt ggagatgttc gaaactacca tccgcttcaa
     3001 accgcgcgac cagtggcgcc ccggcatgac cccggaaaag ttggtcgagg aattggaccg
     3061 cacggtggcg gtgccgggcc tttccaacgt ttgggtgccg ccgatccgta accgcatcga
     3121 catgttggct accggcatca agagcccgat cggagtcaag gtggcgggcg ccaacctcga
     3181 tgatctcgac cgcgtcgccc agcagatcgc ccaagtcgcc aagacagtgc caggggtcag
     3241 ttcggccctg gccgagcggc tgaccggcgg gcgctatgtc gatgtccgca tcgaccgggc
     3301 cgccgccggc cgctacggcc ttaacatcgc cgacgtccag gccatcgtct cgggcgcgat
     3361 cggcggcgag acggtcggcg agaccg
//
LOCUS       DAOMEO010000229         3383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3174_length_3383_cov_2.173377, whole genome shotgun sequence.
ACCESSION   DAOMEO010000229 DAOMEO010000000
VERSION     DAOMEO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_3383_cov_2.173377"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLH31_06480"
     CDS             <1..424
                     /locus_tag="PLH31_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HQR88901.1"
                     /translation="AHCCSPVPGDRIVGILREDGSKDQDGGLDVHTIDCPKLADYEDR
                     EDLWRDLNWTPEAERETVSLTRLHATIQNAPGVLGQVCTIIGEAGGNIVNLRMHHRQS
                     DFFDTDIDVEVRDAKHLTNISAALRACPSVETVDRTRG"
     gene            complement(421..822)
                     /locus_tag="PLH31_06485"
     CDS             complement(421..822)
                     /locus_tag="PLH31_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQR88902.1"
                     /translation="MSDPQRPRVGCGAAILDGQGRLLLVRRIKTPEADHWGVPGGKLD
                     WGEAARRCAEREIAEELGIRIVAGPVLAVTDMIAPDYHWVAITYRTESFEGEPAIQEA
                     HALHEWGWFSLDALPSPLTAATADAVAVLKR"
     gene            903..1487
                     /gene="pyrE"
                     /locus_tag="PLH31_06490"
     CDS             903..1487
                     /gene="pyrE"
                     /locus_tag="PLH31_06490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQR88903.1"
                     /translation="MTNDDVLDEFRAAGGLREGHFVLSSGLHSPVFLQKNLVFMRPER
                     CERLCKALAQKIIATVGQPDVAVSPAVGGIIPGYETARHLNVASIYVEREGGGFKFRR
                     GFHLEPGQKVVMVEDIVTTGLSSRECIAAIQEAGGDVIAAACIVDRSGGKVDVGVPLV
                     SLCSLEVPAYPADALPPELAAIPIEDPGSRRLKG"
     gene            1596..2375
                     /locus_tag="PLH31_06495"
     CDS             1596..2375
                     /locus_tag="PLH31_06495"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQR88904.1"
                     /translation="MSVRLGVNIDHVATIRNARGSSYPEPVRAAELALAAGADGITAH
                     LREDRRHISDADIAVLTDLCLKRGKPLNFEMAVTDEMVGIALKARPHAACLVPERREE
                     VTTEGGLDIVRGYDAIAAATSRLREAGSRVSLFIDAEAAQIQASHAVGAQVIELHTGA
                     YCDAARAGETERAAAILERLKTGAALASSLGLEVHAGHGIDYATVKPIAAIPQIAELN
                     IGHFLIGEAIFVGLPQAIHRMRALMDASRVEHPAKVEAGFA"
     gene            2436..>2501
                     /locus_tag="PLH31_06500"
     CDS             2436..>2501
                     /locus_tag="PLH31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQR88905.1"
                     /translation="MIIGIGSDLCDIRRIEKSLERF"
     assembly_gap    2502..2511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2512..2837
                     /locus_tag="PLH31_06505"
     CDS             <2512..2837
                     /locus_tag="PLH31_06505"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQR88906.1"
                     /translation="FTNKVFTEVERTRSERKPDRASSYAKRFAAKEACAKALGTGLKR
                     GVHLAGMGVVNLPSGKPTMALTGGAAAQLASMVPEGMKAVIHLSLTDDHPYAQAFVII
                     EALPE"
     gene            2982..>3383
                     /gene="lepB"
                     /locus_tag="PLH31_06510"
     CDS             2982..>3383
                     /gene="lepB"
                     /locus_tag="PLH31_06510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQR88907.1"
                     /translation="MTDAADETSPPDEKGGAVQEFIEIIKTVAYALGIALVLRVLLFQ
                     PFTIPSGSMEPNLLEGDYIIVSKFSYGWSKHSIPFSPPLFNGRILGKAPKRGDIVVFK
                     LPRDNRTDYIKRLIGMPGDKVQIRSGVINING"
BASE COUNT          572 a         1159 c         1108 g          534 t
ORIGIN      
        1 cgcccactgc tgcagtccgg tgccgggcga ccgcatcgtc ggcatcctgc gcgaggacgg
       61 ttcaaaggat caggatggcg gcctggatgt ccacacgatc gattgcccca agctggccga
      121 ttacgaagac cgcgaggatc tctggcgcga cctgaactgg acgcccgagg ctgagcgcga
      181 aaccgtgtcg ctgacccgcc tgcacgccac gatccagaac gcgcccggcg tcctgggtca
      241 ggtctgcacg atcatcggcg aagccggcgg caacatcgtc aatctgcgca tgcaccatcg
      301 gcagagcgat ttcttcgaca ccgatatcga cgtcgaggtg cgcgacgcca agcatctgac
      361 caacatctcg gcggcgctgc gggcctgtcc gtcggtcgag accgtggacc ggacgcgcgg
      421 ctagcgcttt agcacggcga ctgcgtccgc tgtcgcggcg gtcagcgggg agggcagggc
      481 gtcgagcgag aaccagcccc attcgtgcaa ggcgtgcgct tcctggatcg ccggttcacc
      541 ctcgaagctt tcggtgcggt aggtgatcgc cacccagtga tagtccggcg cgatcatgtc
      601 ggtcacggcc aggaccgggc cggccacgat ccggatcccc agctcttcgg cgatctcgcg
      661 ttcggcgcag cgccgggccg cttcacccca gtcgagcttg ccgccaggca cgccccagtg
      721 gtcggcctcc ggggtcttga tccgcctgac cagcaagagc cggccctgac cgtcgaggat
      781 ggcggcgccg cagccgacgc ggggacgttg gggatcgctc atgggggtgg atagagccaa
      841 atatgacccg ccgcgcaagg cggcattggc ggactcagcg ccgtgcggta taggcgccgc
      901 tcatgaccaa tgatgacgtc ctcgacgaat tccgcgccgc agggggcctg cgcgaaggcc
      961 atttcgtgct gtcctcgggc ctgcacagtc cggtcttcct gcagaagaac ctggtgttca
     1021 tgcgaccaga gcgctgcgag cgcctgtgca aggccctggc gcagaagatc atcgccacgg
     1081 tcggtcagcc ggatgtggcg gtgtcgccgg ccgtgggcgg gatcatcccc ggctacgaga
     1141 ccgcccgcca tctgaacgtc gcctcgatct atgtcgagcg cgagggcggc ggcttcaagt
     1201 tccggcgcgg cttccacctc gagcccggcc agaaggtcgt gatggtcgag gacatcgtca
     1261 ccaccggcct ttcgtcgcgc gagtgcatcg ccgcgatcca ggaggccggc ggcgacgtca
     1321 tcgccgccgc ctgcatcgtc gaccggtcgg gcggcaaggt cgatgtcggc gtgccgctgg
     1381 tctcgctgtg cagcctggaa gtgccagcct atccggctga cgccctgccg cccgaattgg
     1441 ccgcgatccc catcgaggac cccggcagtc gccggctgaa gggctgagcg agcgctgatt
     1501 ttcaggtctc cacggcgggc ggccaaggcg ctagagtgcc ttggcagtgc ggtcccagag
     1561 cgacttgagg tcacgacatt cctagcgagc atcagatgag cgtccgactg ggcgtcaata
     1621 tcgaccacgt ggcgaccatc cggaacgccc gtggctccag ctatcccgag ccggtgcgcg
     1681 ccgccgaact ggccctggcg gccggggccg acggcatcac ggcccatctg cgcgaggatc
     1741 gccggcatat ttccgacgcc gacatcgcgg tgctgaccga cttgtgcctc aagcgcggca
     1801 agccgctgaa cttcgagatg gccgtcaccg acgagatggt cggcatagcg ttgaaggcgc
     1861 gtccccacgc cgcctgcctg gtgcccgagc gtcgcgagga ggtgactacc gaaggcgggc
     1921 tggacatcgt gcgcggctat gacgccatcg ccgccgccac gagccggctg cgcgaggccg
     1981 gcagccgcgt gtcgctgttc atcgacgccg aggccgccca gatccaggcg tcccacgcgg
     2041 tcggcgccca ggtgatcgag ctgcacaccg gcgcctattg cgatgccgcc cgggccggcg
     2101 agaccgagcg cgccgccgcg atcctggaac gcctcaagac cggcgccgcc ctcgcgtcca
     2161 gcctgggtct tgaggtccat gctggccacg gcatcgacta cgccaccgtc aagccgatcg
     2221 ctgccatccc gcagatcgcc gagctgaaca tcggccactt cctgatcgga gaggcgatct
     2281 tcgtgggtct gccgcaagcc attcatcgga tgcgcgccct gatggacgcg agccgcgtcg
     2341 agcatcccgc aaaagtggag gccggttttg cgtaaggacg ctcgacaaaa tggagagcaa
     2401 gcggatcgcc gttggcgatc cgtggaggtg gctgcgtgat catcggcatc ggctcggacc
     2461 tgtgcgacat ccggcggatc gagaaatcgc tggaacgctt cnnnnnnnnn nggttcacca
     2521 acaaggtctt caccgaggtc gagcgcacgc ggtccgagcg caagcccgac cgcgccagca
     2581 gctacgccaa gcgcttcgcc gccaaggagg cttgcgccaa ggccttgggc accgggctga
     2641 aacgcggcgt tcatttggcc ggcatgggcg tggtcaacct gccgtcgggc aagccgacca
     2701 tggccctgac cggcggcgcg gcggcccagc tggcgtcgat ggtgcccgaa ggcatgaagg
     2761 cggtgatcca tttgtcgctc accgacgacc acccctacgc ccaggccttc gtgatcatcg
     2821 aagcccttcc ggaataacga cttttccgat ccaggcggcg gcgcccaacc gccgccatga
     2881 agccgagtta actggcgagg cgcttgcccc ttcggtaaag cccgtctagc taagcctgtc
     2941 cggtccgccg gaaccctatc gcctgagagc tccgcgcctg catgaccgac gccgccgacg
     3001 agacctcgcc gcccgacgaa aagggcggcg ccgtccagga attcatcgag atcatcaaga
     3061 cggtggccta cgccctgggc atcgccctgg tgctgcgcgt cctgctgttc cagcccttca
     3121 ccatcccgtc gggctcgatg gagcccaacc tgctggaagg cgactacatc atcgtcagca
     3181 agttcagcta tggctggagc aagcactcga tcccgttcag cccgccgctg ttcaacggcc
     3241 gcatcctggg caaggcgccc aagcgcggcg acatcgtcgt cttcaagctg ccgcgcgaca
     3301 atcgcaccga ctatatcaag cgcctgatcg gcatgccggg cgacaaggtc cagatccgca
     3361 gcggcgtcat caatatcaat ggc
//
LOCUS       DAOMEO010000230         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3194_length_3367_cov_2.214674, whole genome shotgun sequence.
ACCESSION   DAOMEO010000230 DAOMEO010000000
VERSION     DAOMEO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_3367_cov_2.214674"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLH31_06515"
     CDS             <1..373
                     /locus_tag="PLH31_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR88908.1"
                     /translation="RQVGSTVGVAVFGAVLTHNLAAPASGAVGSPTAQVHALSLADLE
                     KLAVAGRVAGVVGAAPAVDPRVRETVTHAIQGVIFTGFIVCLLGFLSTLLVPALPLQA
                     RVRPASPTEAPGEPPSGQIEI"
     gene            complement(396..2057)
                     /locus_tag="PLH31_06520"
     CDS             complement(396..2057)
                     /locus_tag="PLH31_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HQR88909.1"
                     /translation="MADAFAAPNRRTLLGAAVVAAGAALTEAPASQASTIQATPTAPA
                     SAAFPIVETTYGKVRGQTNGEVRQFKGIPYGAPTGGRDRFMPPRTPQPWVGVRDALGF
                     GPVSPQGLIDLRADLAMLVQWDRQEGGMGESPLHLNIWTKGLGDGAKRPVVVSLHGGG
                     FESGSGNAPGFDGAQLARYGDVVVVTVSHRLASFGYINLVDNGAPAEFASAGVAGIMD
                     LVASLGWVRDNIAAFGGDPGNVTIFGQSGGGYKTTILLATPAARGLFHRAMVQSGSAL
                     RLTDRETSAKTAAALLAHLGIDKSRSADLQQVPWTTLLEAQTAVAAMPNGGRFSPILD
                     GTYLPHHPFDPGAPAESADIPLIISTTLEDAGLFLFNYNLDEAGLKAQTTAKLGPLGD
                     RAVALYRARYPNKSPFLIQAQMATDSGFRKAAITQAERKAAQSPGKIWMYQWDWETPA
                     YDGKFGAIHGIDVSASFHNYRDGMVGCGVASGRLMCDRLASTLIAFARTGDPNNDHIP
                     AWPAYDAKTRATMIFDNQTRVENDPRSEILGFWNSLPAPPTAAPR"
     gene            complement(2145..2882)
                     /locus_tag="PLH31_06525"
     CDS             complement(2145..2882)
                     /locus_tag="PLH31_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR88910.1"
                     /translation="MAQASPTLKRVHLELGGKSALIIRHDADVQRAAMTVVGMLSVNA
                     GQGCALLTRLLVHNSIRPAFVETVKAFAAMCKIGDPADPTVMMGPLIRESQRAKVEHY
                     IQLGLNSGARLIHGGGRPVGLDKGFFTDLTLFDDVDNSSAIAQDEIFGPVGCIIGFDT
                     DDEAIQIANDSRYGLNGGIETADAATGYAMARRIRAGSVMINGGTGKMSFAPIGGYKR
                     SGVGREYGPDWLKEFTQEKSIFYPVGR"
     gene            2881..>3367
                     /locus_tag="PLH31_06530"
     CDS             2881..>3367
                     /locus_tag="PLH31_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88911.1"
                     /translation="MAGLFGRLAEQQRRKRREGRRFQHQGVAGQQDGRDLRKVQQERE
                     IIARDPRDDSHRFAHDGCAAKDLRPLRVHRIGRRLDRQLHGVVAHGMGHGGAEMVERR
                     LHLHGPGDALRASHLGDDQRRDPGLASVERGMEARHDRDAFGHRHHRPGATVEGPSRR
                     SH"
BASE COUNT          503 a         1144 c         1152 g          568 t
ORIGIN      
        1 ccggcaggtc ggctcgaccg tcggcgtggc cgtcttcggc gccgtgctga cccacaatct
       61 cgccgcgccc gcgtcaggag cggtcgggtc tccgacggct caggtccacg ccctctcctt
      121 ggccgatctc gaaaagctgg ccgtcgccgg tcgcgtcgcc ggcgtggtcg gggccgcgcc
      181 ggccgtcgat cctcgcgtcc gcgagacggt cacccacgct attcagggtg tgatcttcac
      241 cggcttcatc gtctgtctgc tgggtttcct ctcgaccctg ctggtgccgg cgctgccgtt
      301 gcaggcccgc gttcggccgg cttcgccgac cgaagcgccc ggcgagcccc catccggtca
      361 gatcgagatc tgaccggcgc ccctaggccg cccgctcagc gcggcgcggc cgtgggaggg
      421 gctgggaggc tgttccaaaa gcccaggatt tctgatcggg gatcgttctc tacccgcgtc
      481 tggttgtcga agatcatcgt ggcgcgggtc ttggcgtcat aggcgggcca agcgggaatg
      541 tggtcgttgt tcgggtcgcc ggtgcgcgcg aaggcgatca gggtggaggc caggcggtcg
      601 cacatcagcc gacccgacgc cacgccgcag ccgaccatgc cgtcacgata gttgtggaac
      661 gaggctgaaa cgtcgatgcc gtggatcgca ccgaacttgc cgtcataggc tggcgtctcc
      721 cagtcccact gatacatcca gatcttgccg gggctctgcg ccgccttgcg ttccgcctgg
      781 gtgatggcgg ccttccggaa gccgctgtcg gtggccattt gcgcctggat caggaacggc
      841 gatttgttcg gatagcgcgc gcgatacaac gccaccgcgc ggtcgcccag cggccccagc
      901 ttcgcggtgg tctgggcctt cagaccggcc tcgtcgagat tgtagttgaa caggaacaga
      961 cctgcgtctt ccagcgtggt cgagatgatc agcggaatgt cggccgactc cgccggcgcg
     1021 cccggatcga acggatgatg cggtagatag gtcccatcga ggattggcga gaagcgtccg
     1081 ccgttcggca tggccgccac cgctgtctgc gcctccagca gcgtcgtcca gggaacctgt
     1141 tgaaggtcgg cggaacggct cttgtcgatg ccaaggtgag ccagcagcgc cgccgccgtt
     1201 ttggctgacg tctcccggtc ggtcagccgc agcgccgagc cgctttgcac catggcgcga
     1261 tggaacaggc cgcgcgccgc cggggtggcc agcaggatcg tggtcttgta gccaccgccc
     1321 gactggccga agatcgtgac attgccggga tcgccgccaa aagccgcgat attgtcgcgc
     1381 acccacccca gcgaggcgac caggtccatg atccccgcca cgcccgccga cgcgaactcg
     1441 gccggcgcgc cgttgtcgac caggttgata tagccgaagc tggccaggcg atggctgacg
     1501 gtgacgacga cgacatcacc gtaccgcgcc agttgggcgc cgtcgaagcc gggcgcattg
     1561 cccgacccgc tctcgaaccc gccgccatga agcgacacca cgaccggacg cttcgcaccg
     1621 tcgccaagcc ccttggtcca gatgttcaga tgcagcggac tttcgcccat cccgccctct
     1681 tgacgatccc actggaccag catggcgagg tcggcgcgca gatcgatcag gccttgcggg
     1741 ctgacgggac caaagccgag cgcgtcgcga acgccgaccc agggctgggg cgtgcggggt
     1801 ggcatgaagc ggtcgcggcc accggtcgga gcgccatacg gtatgccctt gaactggcgc
     1861 acctcgccat tggtctggcc gcgcaccttg ccataggtcg tctcgacgat gggaaaggcg
     1921 gcgctcgccg gcgcggtcgg ggtggcctgg atcgtcgagg cttgggaggc tggcgcctcg
     1981 gtcagagccg cgccggccgc gacgaccgcc gcgccgagaa gggttcgccg gttcggcgcc
     2041 gcgaaagcat cagccatgtc tcagtctccc tcgggcgcgc cgttgagccg gccttgcgcc
     2101 ttgcttgatc cgccgacggc cggcggggcc tggacgccgc gccgctaccg gccgaccgga
     2161 tagaagatcg atttttcctg ggtgaattcc ttgagccagt caggcccgta ttcgcgcccg
     2221 acgcccgagc gcttgtagcc gccgatgggc gcgaagctca tcttgccggt gccgccgttg
     2281 atcatcacgc tgcccgcccg gatgcggcgc gccatcgcgt agccggtcgc cgcgtcggcg
     2341 gtctcgatgc cgccgttcag gccgtagcgg gaatcgttgg cgatctggat cgcctcgtcg
     2401 tcggtgtcga agccgatgat gcagccgacc ggcccgaaga tctcgtcctg ggcgatggcc
     2461 gagctgttgt cgacgtcgtc gaacagggtc aggtcggtga agaacccctt gtccagaccc
     2521 accggccgac cgccgccgtg gataagcctg gcgcccgagt tgaggcccag ctggatatag
     2581 tgttcgacct tggcgcgctg ggactcgcgg atcagcggcc ccatcatcac cgtcggatcg
     2641 gccggatcgc cgatcttgca catcgcggcg aaggctttga ccgtctccac gaaggcgggg
     2701 cggatgctgt tgtggaccag cagccgggtc agcagggcgc agccttgtcc ggcgttgacc
     2761 gacagcatgc ccaccaccgt catcgcggcg cgctgcacgt cggcgtcgtg gcggatgatc
     2821 agagccgact tgccgcccag ttcgaggtgc acgcgcttca gcgtcggcga cgcttgggcc
     2881 atggccggcc tcttcggccg cctcgccgaa cagcagcgcc gaaaacggcg tgaaggtcga
     2941 cggtttcagc accagggtgt tgccggccag caggatgggc gcgaccttcg aaaggttcag
     3001 caggaacggg aaattatagc ccgtgatccc cgcgacgact cccaccggtt cgcgcatgac
     3061 ggttgtgccg ccaaggatct gaggcccctc cgggttcatc ggattgggcg tcgcctcgat
     3121 cggcaactgc acggtgtcgt cgcgcacggc atgggtcatg gcggcgcgga aatggtcgag
     3181 cggcgcctgc acctgcatgg cccaggtgat gccttgcgag catcccacct cggcgacgat
     3241 cagcgccgcg atcctggcct tgcgagcgtc gagcgcggca tggaggcgcg tcatgaccgc
     3301 gacgcgttcg gccatcgtca tcatcggcca ggggccactg tcgaaggccc gtcgcgccgc
     3361 agccacc
//
LOCUS       DAOMEO010000231         3366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3195_length_3366_cov_2.940803, whole genome shotgun sequence.
ACCESSION   DAOMEO010000231 DAOMEO010000000
VERSION     DAOMEO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_3366_cov_2.940803"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLH31_06535"
     CDS             complement(<1..288)
                     /locus_tag="PLH31_06535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518835.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQR88912.1"
                     /translation="MSMNSVGRPTRPETANDAAGAHETLTGARGLLQDEALIFELGGW
                     DKTGVDLPDASASADDLDSLLRTAPIGLPGLSEPETVRHYVRLSQKNHAIDL"
     gene            complement(285..1631)
                     /gene="gcvPA"
                     /locus_tag="PLH31_06540"
     CDS             complement(285..1631)
                     /gene="gcvPA"
                     /locus_tag="PLH31_06540"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQR88913.1"
                     /translation="MRYLPLTPEDRAQMLGVIGVDSIDALFVDVPASAQRSGTVDLPL
                     HAGELEVEREMAALAKRNRSAGEGPFFCGAGAYRHHVPATVDHIIQRSEFLTSYTPYQ
                     PEIAQGTLQVLFEFQTQVAALTGMEVANASLYDGSTAAAEAVMMATRVTRRNKAVMSG
                     GVHPHYVETIDTLAHAAGVATERLAVAIDAEDAVIAAIDADTACVVVQTPNVFGTATD
                     VTKIAAAAQAAGALLIVVTTEVVSLGLLKSPGEMGADIVVAEGQSIGNGLNFGGPYVG
                     LFACKEKYIRQAPGRLCGETVDADGRRGFVLTLSTREQHIRRDKATSNICTNSGLCAL
                     AFSIHMSLLGEVGLRQLAAINHEKARALRDALAAIPGVEILTPRFFNEFAIRVPGNAA
                     DMVETLAGHGIIAGVPFSRLDAHAGLDDVLLVAATETTLDIDITFFSKVLAKVVAQ"
     gene            complement(1697..2062)
                     /gene="gcvH"
                     /locus_tag="PLH31_06545"
     CDS             complement(1697..2062)
                     /gene="gcvH"
                     /locus_tag="PLH31_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQR88914.1"
                     /translation="MRFTKDHEWVAVEGDVATVGITAYAAEQLGDVVFVEVPEVGKTV
                     KQGDGLAVVESVKAASDVYAPVSGEVVEANTALGDAPETVNSVPEAGGWFAKLKLSNP
                     SEVEALMDRDAYEAFLSTL"
     gene            complement(2133..>2247)
                     /locus_tag="PLH31_06550"
     CDS             complement(2133..>2247)
                     /locus_tag="PLH31_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HQR88915.1"
                     /translation="PALSAIGTKLKVIVRGKPQAAEVVASPFVPNRYVRKI"
     assembly_gap    2248..2257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2258..3178)
                     /gene="gcvT"
                     /locus_tag="PLH31_06555"
     CDS             complement(<2258..3178)
                     /gene="gcvT"
                     /locus_tag="PLH31_06555"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQR88916.1"
                     /translation="MVPFAGYSMPVQYKDGVLKEHLWTREHAGLFDVSHMGQARIRGE
                     NPARSFEKLVSADYQGLKPGKQRYAVLLNAEGGVIDDLMTARPDDDGLFVVVNGACKD
                     NDYAIIAKALEGEATVERLEDRALLALQGPEAAAVLAVHAPQAAGMVFMDTAAMTAFG
                     VDAIISRSGYTGEDGYEISVPAAEATRIWNTLLEDERVKPIGLGARDSLRLEAGLPLY
                     GHDMDETVSPIEAGMNFAIGKSRREAGDYLGVDRISRELAGDLKRVRVNLRVLEGAPA
                     REGAEIADETGAVVGVVTSGGFGPSVGGAIA"
BASE COUNT          548 a         1085 c         1152 g          571 t
ORIGIN      
        1 caggtcgatg gcgtggttct tctggctcag gcgcacatag tggcgcaccg tctcgggctc
       61 ggagaggccc ggcaggccga tcggggcagt ccgcagcagg ctgtccaggt cgtcggcgga
      121 cgccgaagcg tccggcaggt cgacaccggt cttgtcccag ccgcccagtt cgaagatcag
      181 ggcttcgtcc tgcaacagtc cgcgcgcacc ggtcagggtc tcatgagcgc cagcggcgtc
      241 attggcggtt tcgggacggg tcggccgtcc tacgctgttc atgctcattg ggcgacgacc
      301 ttggccagga ccttggagaa gaaggtgatg tcgatatcga gggtggtctc ggtggcggca
      361 accagcagaa catcgtcaag gccagcatgg gcgtcgaggc gcgagaacgg caccccggcg
      421 atgatgccgt gacccgccag ggtctcgacc atgtcagcgg cgttgccagg cacgcgaatg
      481 gcgaactcgt tgaagaaacg cggcgtcagg atctcaacgc cgggtatagc ggccagggcg
      541 tcgcgcaggg cgcgggcctt ttcgtgattg atcgcagcaa gctggcgcag gccaacctcg
      601 cccaacaggc tcatgtgaat gctgaacgcc agggcgcata gaccgctgtt ggtgcagatg
      661 ttgctggtcg ccttgtcgcg acggatatgc tgctcgcggg tcgagagcgt cagcacaaag
      721 ccgcgccggc cgtcagcgtc caccgtttcg ccgcagagac ggcccggagc ctggcggatg
      781 tatttttcct tgcaggcgaa caggccgaca tagggaccgc cgaagttcag gccgttgccg
      841 atcgactggc cctcggccac gacgatatcg gcgcccattt cgccgggtga cttcagcaga
      901 cccagggaca cgacctcggt cgtcaccacg atcagcagcg cgccagcggc ctgggcggcg
      961 gcggcgattt tggtcacatc ggtggccgtg ccgaacacat tgggggtctg aacgacgacg
     1021 caggcggtgt cggcgtcgat ggcggcgatc acggcgtcct cggcgtcaat ggcgacggcc
     1081 aggcgctcgg tggcgacgcc ggccgcgtgg gccagggtgt cgatcgtctc gacatagtgc
     1141 gggtggacgc cgcccgacat caccgccttg ttgcggcggg tgacgcgggt tgccatcatc
     1201 accgcctcgg cggcggcggt ggacccgtca tagagcgagg cgttggccac ttccatgccg
     1261 gtcagggccg cgacctgggt ctggaattcg aacagcacct gcagcgtgcc ctgggcgatt
     1321 tccggctggt agggcgtgta gctggtcagg aactctgacc gctggatgat gtggtcgacc
     1381 gtggctggca cgtggtggcg ataggccccg gcgccgcaga agaacggccc ctcccccgcc
     1441 gagcggttgc gtttggccag agccgccatc tcgcgctcga cctccagttc gccggcgtgc
     1501 agcggcaggt cgacggttcc ggaacgctgg gcggaggccg gcacgtcgac aaacagggcg
     1561 tcaatcgagt ccacgccgat gacgccaagc atctgcgcgc ggtcttctgg agtgaggggc
     1621 aggtaacgca tggggaggag cgcctaggtc tgtgtcgaac cgagcgctcg tcaggagcgc
     1681 ccggtcgact cgtcaatcag agggtcgaga ggaaggcttc gtaggcgtca cggtccatca
     1741 gggcctcgac ttccgaaggg ttggacagct tcagcttggc gaaccaaccg ccggcttcgg
     1801 gaaccgaatt gacggtctcg ggcgcgtcgc ccagggcggt attggcctct acgacctcgc
     1861 cagataccgg ggcgtagaca tccgaagcgg ccttcacgct ctcgaccacg gcaaggccgt
     1921 cgccctgctt gacggtcttg ccgacttcgg ggacctcgac gaacaccacg tcgcccagtt
     1981 gctcggcggc gtaggcggtg atgccgacgg tggcgacgtc gccttcgacg gcgacccatt
     2041 catggtcttt ggtgaagcgc atcaggaggc ccttttctct ttgttgtcgc gcgacggacg
     2101 ccaaaaccgg catccacttt tggctgtcgc gcttagatct tgcggacgta acgattggga
     2161 acgaacggcg aggccacgac ctccgccgcc tgcggcttgc cgcgaacgat gactttcagc
     2221 ttcgtgccga tggccgaaag ggctggcnnn nnnnnnnggc gatggcgccg ccgacgctgg
     2281 gaccaaagcc gccgctggtg accacgccga ccacggcgcc ggtctcatcg gcgatctccg
     2341 cgccttcgcg ggccggggcg ccctccagga cacgcagatt gacccgaacg cgcttgaggt
     2401 cgccggccag ctccctggaa atgcgatcga cgcccagata atcgccggcc tcgcgacggc
     2461 tcttgccgat ggcgaagttc atgcccgctt cgatcggcga gaccgtctcg tccatatcgt
     2521 ggccatagag cggcagaccg gcctccagac gcaggctgtc gcgcgcgcca aggccgatcg
     2581 gcttgacccg ctcgtcctcc agcaaggtgt tccagatgcg ggtcgcttcg gcggcaggga
     2641 ccgagatctc gtaaccgtcc tcgccggtat agcccgagcg cgagatgatg gcgtcgacgc
     2701 cgaaggcggt catcgcggcg gtatccatga agaccatgcc tgcggcttgc ggcgcgtgga
     2761 cagccagcac cgcggcggcc tcgggcccct gcagcgccag aagcgcgcgg tcctcgagac
     2821 gttcgaccgt agcctcgccc tcgagggcct tggcgataat ggcgtaatcg ttgtccttgc
     2881 aggcgccgtt gacgaccacg aacagaccgt cgtcgtcggg ccgcgcggtc atcaggtcgt
     2941 cgatcacgcc gccctcggca ttcagcagta cggcgtaacg ctgcttgccg ggcttgaggc
     3001 cctgatagtc ggccgagacc agtttctcga agctcctggc cgggttctcg ccccggatgc
     3061 gggcctggcc catgtgcgag acgtcgaaca ggccggcatg ctcgcgggtc cacagatgct
     3121 ccttgagcac cccatccttg tactgcaccg gcatggaata acccgcgaac ggaaccatgc
     3181 gggcgccggc ggcgacgtgc gcatcgtaca gggacgtctt cttgagatct tggtcggaca
     3241 cgcgggggct ccaaacgggg acggcgctgc tagcccggct ttcaccagac gatagcgccc
     3301 ccgctgtccc tttgacctga gagattccgg cccgcatacg ggccttgctc cttcggcggg
     3361 cagtcc
//
LOCUS       DAOMEO010000232         3353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3210_length_3353_cov_2.764099, whole genome shotgun sequence.
ACCESSION   DAOMEO010000232 DAOMEO010000000
VERSION     DAOMEO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_3353_cov_2.764099"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>954)
                     /locus_tag="PLH31_06560"
     CDS             complement(<1..>954)
                     /locus_tag="PLH31_06560"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HQR88917.1"
                     /translation="LANGVVLTVLLWTDRARWPLLIACAMTGNIVASLHAGGQIPVVL
                     GLSFANVVEYLLSAVALRHVLGRDIDVSRSRDLGWIALICGLCAPTVSGLIATAVLHV
                     SRNADIGRTFVGWGLSNALGLMLVTPCLIAFRQARSLLAERPVTRRGWLALAVLVVTI
                     IVVFTRPRYPLLFIIPPILVFVALELEFLGAAVGILVLSSMAIGFTALNIGPVAVTAQ
                     DMAERALFLQIFLVVSIFSALPLATINAHRRRLRDVAQEQTRWADMAENLAGVGYWRV
                     VLATQTVTWSDQMYAILGVQPASRITPNQWFKAIHPDDLVK"
     assembly_gap    955..964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1304..>1745
                     /locus_tag="PLH31_06565"
     CDS             1304..>1745
                     /locus_tag="PLH31_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HQR88918.1"
                     /translation="MRLLAALAICLFTVLPVAAAEPLDPAPRIAVISAFPPELTALAA
                     ATSNQKRYTAAGVTFTTGELEGKPIVLFLSGVSMVNAAMTTQLALDRFNITRIVFSGI
                     AGGVDESLDIGDVIVADQWSQNLETAFARETEKGFEISPSASNDT"
     assembly_gap    1746..1755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1756..2207
                     /locus_tag="PLH31_06570"
     CDS             <1756..2207
                     /locus_tag="PLH31_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HQR88919.1"
                     /translation="GMMIPRSIMIARADGPPERRRWFPADPAMLETARSIASTVSLAR
                     CVTNQCLVHAPKVVVGGNGISAPVFLDNAAYRKYARATFQARVVDMESAAVAHVAYAN
                     GAPFIAFRSLSDLAGGGDEANEMHTFLQLASDNSASVVKAFVKALPN"
     gene            complement(2204..2833)
                     /gene="upp"
                     /locus_tag="PLH31_06575"
     CDS             complement(2204..2833)
                     /gene="upp"
                     /locus_tag="PLH31_06575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQR88920.1"
                     /translation="MSTVTVVSHPLVQHKLTRMRDRTTSTKTFRALMRETATLICYEV
                     TRDLPMDEVQIETPVASTTAREIAGKKLVFAPILRAGLGMADGMLDLVPSARVAHVGL
                     YRDPESLEAVEYYFKAPQDVAGRLVIVVDPMLATGHSAIAAITRLKAAGVSDLRFVCL
                     LAAQVGVDNLRAAHPDVPIWTAAIDAQLNDHGYIVPGLGDAGDRTFGTR"
     gene            complement(2884..>3353)
                     /locus_tag="PLH31_06580"
     CDS             complement(2884..>3353)
                     /locus_tag="PLH31_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR88921.1"
                     /translation="EKSGWGRKTSKGTGLGVACHYSHLGYVAVVMEVSVSKGALKIHK
                     VWAAADVGSQIVNPSGAEQQVQGSILDAIGATLHQAITFENGAAVQSSFADHPLLRMT
                     EAPPVEVHFKLSDNRPTGLGEPAYPPAPAALCNAIFAATGKRIRSLPIGDQLA"
BASE COUNT          597 a         1117 c         1084 g          535 t
ORIGIN      
        1 tcttcaccag atcgtcgggg tgaatcgcct tgaaccattg attgggcgta atgcgacttg
       61 ccggctgaac gcccaggatc gcgtacatct ggtcagacca ggtcaccgtc tgggtggcaa
      121 ggaccacgcg ccagtagcca actccggcca gattctcggc catgtcggcc catcgggtct
      181 gttcctgcgc gacgtcgcga agacgccgtc tgtgagcgtt gatcgtcgcc agcggcaagg
      241 cggaaaagat cgagaccacc aggaagatct gcaggaacag cgcgcgctcg gccatgtcct
      301 gagccgtcac ggcgacggga ccgatattca gcgccgtgaa gccgatagcc atgctggaca
      361 gaacaaggat tccgaccgcc gccccgagga actccagctc cagagcgacg aaaaccagga
      421 ttggcgggat gatgaacagc agcggataac gtggccgggt aaagacgacg atgatggtga
      481 ccaccagcac cgccagggcc agccaaccgc gccgggtgac cggacgctcg gccagaagcg
      541 agcgcgcctg gcgaaaggct atcaggcagg gcgtcaccag catcaggccg agggcgttgg
      601 acaggcccca accgacgaag gtgcgaccga tatcggcatt tcgcgagaca tggagaacgg
      661 ccgtcgcgat caatcctgac accgttggcg cgcacaggcc gcagatcagg gcgatccagc
      721 ccagatcgcg cgaccggctg acatcgatgt cgcgacccag cacatgccgc agagccaccg
      781 ccgatagcag atactcgacc acattggcga agctgagccc cagaacgacg ggaatctgtc
      841 cgcccgcatg caggctggcc acgatattgc ccgtcatggc gcaggcgatg agcaggggcc
      901 agcgcgcccg gtccgtccag agcagaaccg tcagcacgac cccattggcc agccnnnnnn
      961 nnnngatcgc gcggcagctc gatgcagaca taggccagcg ccatgacagc cagccccaag
     1021 gccaccgcag cggcgatctg ggcggtccgc gcggtcgcgt aggcaggtgt cgtggctcgg
     1081 ttcatgctct agtatcctgc cagtcctgtc tgcatcactg gttaacctgg cggaaatcta
     1141 tcgcggcccg cgccggtgac cgcccacctt gcagaaacgg cggcgggcga ctctcgcttg
     1201 aacagattgt cgacgattgg cccaggatcg ccagcgagct atgtgcaggc tctccatgtc
     1261 gtgctgcctt cggccgcccc gttccccgag agagagtccc tccatgcgtt tgcttgcagc
     1321 cctcgccatc tgcctcttca ccgttctccc ggtggccgcg gccgagccgt tggaccccgc
     1381 gccgcgcatc gcggtgatct ctgccttccc gccggagctg acggccctcg ccgcagcgac
     1441 cagcaaccag aagcgctata cggctgccgg cgtgacgttc acgacgggag agcttgaggg
     1501 caagcccatc gtgctgttcc tcagcggagt cagcatggtc aatgcggcga tgaccaccca
     1561 gctggccctg gatcgtttca acatcacccg catcgtcttc tcagggatcg ccggaggcgt
     1621 tgatgaaagc ctcgacatcg gtgacgtgat cgtggccgac cagtggagcc agaatctgga
     1681 aaccgccttc gcccgcgaga ccgaaaaggg cttcgagatt tcgccatccg ccagcaacga
     1741 cacccnnnnn nnnnnttggc atgatgatcc cccgctcgat catgatcgcc cgcgccgatg
     1801 gcccgcccga gcgccgtcgc tggttcccgg cggatccggc catgctggag accgcgcggt
     1861 ccatcgcctc aacggtctcg ctggcccgct gcgtgaccaa tcagtgcctg gtccatgcgc
     1921 ccaaggtggt ggtcggcggc aacggcatct cggcgccggt gttcctggac aacgccgcct
     1981 accgaaaata tgcccgcgcc accttccagg cccgggtagt ggacatggag agcgccgctg
     2041 tcgcccacgt ggcctatgcc aatggcgcgc cgttcatcgc cttccgcagc ctgtcggacc
     2101 tggccggcgg cggcgacgaa gccaacgaga tgcatacgtt cctgcaactg gcctcagaca
     2161 actcggccag cgtggtcaag gccttcgtga aggcgctgcc gaactagcgc gtcccgaacg
     2221 tccggtcgcc ggcgtcgccc aggccgggca cgatatagcc gtggtcgttg agctgggcgt
     2281 cgatggcggc ggtccagatc ggcacgtcgg gatgggcggc ccgcaggttg tcgaccccga
     2341 cctgcgcggc cagcaggcac acgaagcgca ggtcgctgac cccggccgcc ttcaggcggg
     2401 tgatcgcggc gatggccgaa tggccggtgg ccaacatcgg atcgaccacg atgaccagac
     2461 ggccggccac gtcctgcggg gccttgaaat agtattcaac cgcctccagc gactccggat
     2521 cgcgatagag gcccacatgg gcgacgcggg ccgagggcac caggtccagc atgccatcgg
     2581 ccatgcccag gccggcccgc aggatcggcg cgaacaccag cttcttgccg gcgatttccc
     2641 tggccgtggt cgaggcgacc ggagtctcga tctggacctc gtccatcggc aggtcgcggg
     2701 tgacctcgta gcagatcagg gtcgccgtct cgcgcatcag ggcgcggaag gtcttggtcg
     2761 aggttgtcct gtcccgcatc cgggtcagct tgtgctggac gagcggatgg ctgacgaccg
     2821 tgacggtgga catggaagac ccctgctaaa agcgcgttct cgctcctagc aggggtcttg
     2881 ccgtcaggcc agttggtcgc cgatcggcag ggaccggatg cgcttgcccg tcgccgcgaa
     2941 gatggcgttg cacagggccg ccggagccgg cggataggct ggctcgccga ggccggttgg
     3001 ccggttgtcg ctgagcttga aatgcacctc gaccggcggc gcttcggtca ttcgcagcag
     3061 cgggtggtca gcaaagctgc tctggacggc cgcgccgttc tcgaaggtga tcgcctgatg
     3121 cagggtcgcg ccgatggcgt ccaggatcga gccctgcacc tgctgctcgg ccccgctggg
     3181 gttgacgatc tgcgaaccca catcggcggc ggcccagacc ttgtgaatct tcagcgcacc
     3241 cttgctgacg gaaacctcca tcacgacggc gacataaccc agatggctgt agtggcaggc
     3301 gacgcccagt cccgtgccct tcgaggtctt gcggccccag ccggacttct cgg
//
LOCUS       DAOMEO010000233         3352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3213_length_3352_cov_2.412800, whole genome shotgun sequence.
ACCESSION   DAOMEO010000233 DAOMEO010000000
VERSION     DAOMEO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_3352_cov_2.412800"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..198
                     /locus_tag="PLH31_06585"
     CDS             82..198
                     /locus_tag="PLH31_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88922.1"
                     /translation="MPAVRLFRIADKVMMTLITAALIVGVPVSAITFLAQSL"
     gene            271..804
                     /locus_tag="PLH31_06590"
     CDS             271..804
                     /locus_tag="PLH31_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HQR88923.1"
                     /translation="MRALGPGSVSSFLKIILDVIYVGLWIFVSLLSLFTLVALLFSFN
                     PELLAQMLPISDAVEAVSRDGPLFAGALAAWALLLGGWMVIVERLRRIFATLTAGDPF
                     HPDNVLRLRVVGLILASLEVGRYVFSGLARWLAPKTKVIDDSFTLTAWFSVLVVFVLA
                     EVFREGARLRREAELTI"
     gene            825..1049
                     /locus_tag="PLH31_06595"
     CDS             825..1049
                     /locus_tag="PLH31_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR88924.1"
                     /translation="MPVRVNLDRVLLDRRMSLTELSDRVGVTIANLSILKTGKARAVR
                     FSTLDALCRELDCQPGDLLCFESGPPDSDD"
     gene            1103..2989
                     /locus_tag="PLH31_06600"
     CDS             1103..2989
                     /locus_tag="PLH31_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQR88925.1"
                     /translation="MTPDEAKARLATTWVASRAFVSRHRIAVGVSAGVLAVLVLLFVW
                     LADRLPISQALEPLPNRAIILLDNQGKPFARRGAYKEAPVVIGALPQHVPAAFLSTED
                     RRFYSHIGVDFRGIARAMRANSRAGRTVEGGSTITQQLAKNAFLEPERSWRRKAQEAV
                     IAVYLELRLSKDEILARYLSSIYFGDGVYGLGAAARHYFGKAPEALSIGEAAMLAGMV
                     KAPVNLNPVDHPKAAGARARLVLEAMVATKAITRQQADTSGKVVVRTTRADLPVGGYF
                     ADWISPQVKSAFDGPGYGEVRVTTTLDSRLQRIAERAVANRMKGAAKVKAGQVALVAM
                     RPDGQVVAMVGGQNYRQSPFNRAVQAQRQPGSAFKLFVYLAALRDGASPDDLIDAGRI
                     TIGGWTPANYEGGEGYDLTLRDAFAQSSNTAAARVWQTAGGPAVVQAAHDLGIDSPLK
                     ADDATLSLGTAETNLLELTSAYAAVAAGKGPSFPHGRPFKSPPPQAKLDPGQREALLD
                     LLAAVVQEGTGRAARLSQRAYGKTGTTQDHRDALFVGFTGDLVVGVWIGNDDQTPMNR
                     VVGGGLPALIWRDVMTGGLKAGLIAKDRAPRPRFEPGPEREAEEEFDPERRVPRWLRR
                     ILGL"
BASE COUNT          512 a         1164 c         1080 g          596 t
ORIGIN      
        1 gtgcaaatcg gggcctatca ctgggtcatg gacgccgccg gccttggcaa gtgtccgcca
       61 cctcgatgga tccaccccca aatgcccgcc gtccgactgt tccgcatcgc cgacaaggtc
      121 atgatgaccc tgatcaccgc cgccctgatc gtgggcgtcc cggtctcggc gatcaccttc
      181 ttggcgcaat cgctctaggg gttgtttgag gccccgctgt aacgcggggc aggcttggga
      241 gccctgggga gggcgaggga aagacagcca atgcgcgctt tggggccagg ctcggtatcg
      301 agctttctta agatcatcct cgacgtcatc tatgtcggcc tttggatttt cgtcagcctg
      361 ctgtcgctgt tcaccctggt ggccctgctc ttcagcttca atcccgaact tctggcccag
      421 atgttgccga tcagcgacgc ggtcgaggcc gtgagccgcg acggtccgct gttcgccggc
      481 gccctggccg cctgggcttt gctgctgggc ggctggatgg tgatcgtcga gcggctgcgc
      541 cggatcttcg ccaccctgac cgccggcgat cccttccatc ccgacaatgt cctgcgcctg
      601 cgggttgtgg gtctgatcct ggccagcctg gaggtcggac gctacgtctt ctcgggtctg
      661 gcccgctggc tggcccccaa gaccaaggtg atcgacgaca gcttcaccct gaccgcctgg
      721 ttctccgtgc tggtcgtatt cgtcctggcc gaggtcttcc gcgagggcgc ccgccttcgt
      781 cgcgaagccg aactcaccat ctgatcgcgg agagatttcg ccccatgccc gtccgcgtga
      841 acctcgatcg cgtcctgctc gaccgacgca tgtccctgac cgaactgtcc gaccgggtgg
      901 gcgttaccat cgccaacctg tcaatcctga agaccggcaa ggcccgggcc gtgcggttct
      961 cgaccctgga cgccctgtgc cgcgagctgg actgccagcc cggcgacctg ctgtgcttcg
     1021 aatccggccc gcccgacagc gacgactgaa ccagcgccct ccccacccgt tccaggcagc
     1081 ttgagcggcg gaaaggtcgg gcatgactcc tgacgaagcg aaggccaggc tggcgaccac
     1141 atgggtcgcc tcgcgagcct tcgtgtcgcg ccatcgcatc gccgtcggcg tatcggcggg
     1201 cgtgctggcg gtcctggtcc ttctgttcgt ctggctggcc gaccgcctgc cgatctcgca
     1261 agcgttggag cccctaccca accgcgcgat catcctgctc gacaaccagg gcaagccctt
     1321 cgcccggcga ggcgcctaca aggaagcgcc agtcgtcatt ggcgccctgc cccagcatgt
     1381 gccggccgct ttcctgtcca ctgaggaccg acgcttctac agccacatcg gcgtggactt
     1441 tcgcggcatc gcgcgcgcca tgcgggccaa ttcccgcgcc ggccgcacgg tcgaaggcgg
     1501 cagcaccatc acccagcaac tggccaagaa cgcctttttg gagcctgaac ggtcctggcg
     1561 ccgaaaggcc caggaggcgg tgatcgccgt ctatctggaa ctacggctgt ccaaggacga
     1621 gatcctggcc cgctatctct ccagcatcta tttcggcgac ggggtctatg gcctgggcgc
     1681 ggcggcccgg cactatttcg gcaaggcgcc ggaggccctg tcgatcggcg aggcggcgat
     1741 gctggccggc atggtcaagg cccccgtcaa cctcaatccg gtggaccatc caaaagccgc
     1801 cggagctcgc gcacggctgg tgctggaggc catggtcgcg acaaaggcca tcacccgtca
     1861 acaggccgac acgtccggca aggtcgtcgt ccgcaccacc cgcgccgacc tgccggtggg
     1921 cggttatttc gccgactgga tttcgcctca ggtgaagtcg gccttcgacg gtccgggcta
     1981 tggcgaggtg cgggtgacca ccaccctcga cagccgcctg cagcgcatcg ccgaacgggc
     2041 ggtcgccaat cggatgaagg gcgcggccaa ggtcaaggcc ggccaggtcg cgttggtcgc
     2101 catgcgaccc gacggtcagg tcgtggccat ggtcggcggc cagaactatc gccaaagccc
     2161 attcaaccgc gccgtccagg cccagcgcca gccgggcagc gctttcaagc tcttcgtcta
     2221 tctggcggcc ctccgcgacg gcgcctcgcc tgacgacctg atcgacgctg gccgcatcac
     2281 catcggcggc tggacccccg caaactacga gggcggcgag ggatacgacc tgaccctgcg
     2341 cgacgctttc gcccagtcga gcaacacggc cgcagcgcgg gtctggcaga cggctggagg
     2401 gccggctgtc gttcaggcgg ctcacgacct ggggatcgac tcacccctca aggccgatga
     2461 tgcgacacta tccctgggca cggcagagac caacttgctg gaactgacct ccgcctacgc
     2521 tgctgtggcg gcgggaaaag gcccgtcttt cccccacggt cgtccgttca agtctccgcc
     2581 gccgcaggcc aagctcgacc ccggccagcg cgaggcgttg ctcgatctgc tggccgcggt
     2641 ggttcaggag ggcacgggtc gcgccgcccg cctgtcccag cgggcgtatg gcaagaccgg
     2701 caccacccag gaccatcgcg acgccctgtt cgttggcttc actggcgacc tggtcgtggg
     2761 ggtctggatc ggcaacgacg accagacgcc gatgaaccgg gtcgttggcg gcggtctgcc
     2821 agccctgatc tggcgtgacg tcatgacagg cggactgaag gcgggactga ttgccaagga
     2881 ccgcgcgccc agaccgcggt tcgagccagg acctgaacgt gaggcggaag aggaattcga
     2941 cccggagcgg cgcgtcccca gatggctcag gcgcatcctg ggtctttgag gcgccacgcc
     3001 gccagtccag ggggccggcg gcgttcaggc ggagatctat ttcttcttga acagggtcgc
     3061 catatcgccc ttcacgctgg ggtttgacgc gttggctttc ggcggagccg gcttcttcgc
     3121 cttgcccttg gtctctttca tgatgcgtct ttcctttggt cgcgatcggc ttggcgactc
     3181 gccgtcgatc ttgaccgccg ccggtcactc acgacacggc gagagagcag tgtggcacgg
     3241 acagagcacc cgcgggcttt aatcgcctac agcggcgagc gggcgcggac ttcacgcccg
     3301 gccgctccgg tcgtgtcgat cactgaaccc ggcagggcgc cagcctcggt tt
//
LOCUS       DAOMEO010000234         3345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3219_length_3345_cov_2.503040, whole genome shotgun sequence.
ACCESSION   DAOMEO010000234 DAOMEO010000000
VERSION     DAOMEO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_3345_cov_2.503040"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1005
                     /locus_tag="PLH31_06605"
     CDS             98..>1005
                     /locus_tag="PLH31_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88926.1"
                     /translation="MSDLITAWSPEQKAAFGKRPMTFHHGLADSRLFEDDALARVLDA
                     YPAELYDINLFDFDADGQHTMRTGARGGRSGAEVLEGVKEGRIWVQLRRAEAWYPQLG
                     DLIQQAFAGISGQAKGFKPIQMSGQLILSAPGAKVPFHADAPGVVLFHLRGRKRIWIY
                     PADEAHMPQQGMENILLKQQTEDLPYRRDMDAAAQVFDLEPGMAAAWPLHAPHRIENL
                     GTFNVSLSVDYQTWGSRLINGAHYANGVFRRWGMPVAAMEKTPVLARGALWAASLGLK
                     RARLVEDRIKGLERSFELGTDAPAEAR"
     assembly_gap    1006..1015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1116..1955
                     /locus_tag="PLH31_06610"
     CDS             1116..1955
                     /locus_tag="PLH31_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HQR88927.1"
                     /translation="MRALLVLAAGAAALGFAGAASAAPSVRIKDAVARVVVIPEARSD
                     IKVEFITTNKSLPLTVRQNGPDTIVDGDLRMNKIRGCNSNMGKTTIKVRGVGEVKWDD
                     IPQIVVRTPMNVDVAGAGAVFGSVGRSDALELANAGCGEWTVANVKGKLEVSQAGSGN
                     TNVGSAGSAEISIAGSGNVRTQQVAGDFTVSIAGSGNAGAASVGGKVEVNIAGSGDVA
                     IGGGNSRSVEVSIMGSGDVSHDGEVGDVEVSVAGSGDVRIAKATGNVSKSVAGSGDVY
                     IGK"
     gene            complement(2047..2805)
                     /locus_tag="PLH31_06615"
     CDS             complement(2047..2805)
                     /locus_tag="PLH31_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88928.1"
                     /translation="MKTQLFAAAAVAVLAVSAPAFAQQVGSVGAAVNYTDAETNAFNA
                     DGTSVKIDGSVAVPVGSAWTVTVNGDVSISDNDISDETVASGAAHLTTKIGDSWRVGG
                     FTALSRPSNDTLWAVGAEAHKYLGNVTLSGLAAYGQSNDLDADLYAVRGEARYFVTDD
                     FRLDAGLGYSKIDTTVEADTWDASLGGEYKFANTGWSTFGKYTHTESEDLADLKSDAV
                     KIGLRYTFGGSLKARDAAGADLIDAGTLFGFGVR"
     gene            3189..3345
                     /locus_tag="PLH31_06620"
                     /pseudo
     CDS             3189..3345
                     /locus_tag="PLH31_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516677.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          571 a         1016 c         1150 g          598 t
ORIGIN      
        1 gccatgaccc gccgccatcc tcgcatcggc gcgggtgtcg gcctcggggt cggtcttggc
       61 ctgagcctca ccctcttggt cgactagggg gatttcaatg tccgatctga tcacggcctg
      121 gtcgcccgag cagaaggccg cctttggcaa gagaccgatg accttccacc atgggctggc
      181 ggactcacgg ctattcgagg acgacgccct ggcgcgcgtg ctggacgcct atccggccga
      241 gctctacgac atcaatctgt tcgactttga cgctgacggt cagcacacca tgcgcaccgg
      301 cgctcggggc gggcggtccg gggctgaagt gctggaaggc gtgaaggaag gtcggatctg
      361 ggtgcagcta cgccgggccg aggcctggta cccgcagctg ggcgacctga tccagcaggc
      421 ctttgcgggg atctccggtc aggccaaggg cttcaagccg atccagatga gtggccagct
      481 gatcctgtcg gcgccgggag ccaaggtgcc tttccacgcc gacgcccccg gcgtggtgct
      541 gttccacctg cgtgggcgca agcggatctg gatctatcca gccgacgagg cccacatgcc
      601 gcagcagggc atggagaaca tcctgctcaa gcagcagact gaagacctgc cgtatcgccg
      661 cgacatggac gctgcggccc aggtgttcga cctggagccc ggcatggccg ccgcctggcc
      721 gctgcacgcg ccgcaccgga tcgagaacct ggggacgttc aatgtctcgc tgtcggtgga
      781 ctatcagacc tggggctcgc gcctgatcaa cggcgcgcac tatgccaacg gcgtcttccg
      841 gcgctggggc atgccggtgg cggcgatgga gaagacaccc gtcctggccc gcggcgcgct
      901 gtgggccgcg tccctgggct tgaagcgcgc gcgtctggtc gaggaccgga tcaaggggtt
      961 ggagcgcagt ttcgaactgg gaactgacgc accggccgaa gcacgnnnnn nnnnnatttg
     1021 tgacatcgcg gtcatgacct cgaattaagt cgtgtcagtc ggcgaccatc gttagctttc
     1081 ccccatcaac gccatccgaa ggggaaaata tctcgatgcg cgcacttctc gttctggccg
     1141 ccggcgcggc cgccctgggt ttcgctggcg ccgcctcggc cgcgccttcg gtccgtatca
     1201 aggacgcggt cgcccgtgtc gtggtgatcc ccgaagcccg cagcgacatc aaggtcgagt
     1261 tcatcaccac caacaagagc ctgccgctga ccgtccgtca gaatggtccg gacacgatcg
     1321 tcgacggcga tctgcgcatg aacaagattc ggggctgcaa cagcaacatg ggcaagacca
     1381 ccatcaaggt gcgtggcgtc ggtgaagtga agtgggatga cattccgcag atcgtcgtgc
     1441 gcacgccaat gaacgtcgat gtcgctggcg ctggcgcggt gttcggcagc gtgggtcgca
     1501 gcgacgccct ggaactggcc aatgccggtt gcggcgaatg gaccgtcgcc aatgtgaagg
     1561 gcaagcttga ggtttctcag gcaggttcgg gcaataccaa tgtcggctcg gcggggtcgg
     1621 ccgagatcag cattgccggt tctggcaatg tgaggaccca gcaggtggca ggcgacttca
     1681 cggtttcgat cgccggctcc ggcaatgccg gcgcggccag cgttggcggc aaggtcgagg
     1741 tcaatatcgc cggttccggc gacgtggcga tcggcggcgg caattcgcgc tctgtcgagg
     1801 tcagcatcat gggctcgggc gacgtcagcc atgacggcga agtcggcgat gtggaagtgt
     1861 cggtggcggg ttcgggcgac gtgcggatcg ccaaggcgac cggcaatgtc agcaagtcgg
     1921 tcgccggctc gggcgacgtc tatatcggta aataggccgg cgttcgccga ccgcgcctat
     1981 atatagagtg aaggccccgg gacgatgtcc cggggccttc cgcttttcag gcgctagccg
     2041 tccgctttag cggacgccga agccgaacag cgtaccggcg tcgatcagat cggcgccggc
     2101 tgcgtcgcgg gccttcaggc taccgccgaa ggtgtagcgc aggccgatct tgaccgcgtc
     2161 cgacttcagg tcggccaggt cttcgctttc ggtgtgggtg tacttgccga aggtcgacca
     2221 gccggtgttg gcgaacttgt attcgccgcc caggctcgcg tcccacgtat cggcttcgac
     2281 ggtggtgtcg atcttcgaat agccgagccc agcgtccaga cggaagtcgt cggtgacgaa
     2341 gtagcgggct tcaccgcgca cggcgtagag gtcggcgtcc aggtcgttgg actgaccata
     2401 ggctgccagg cccgacaggg tcacgttgcc caggtacttg tgggcttcgg cgccgacggc
     2461 ccagagggtg tcgttgctcg gacgcgacag ggcggtgaag ccgccgacgc gccagctgtc
     2521 gccgatcttg gtggtcaggt gggctgcgcc cgaggcgacc gtctcgtcgc tgatgtcgtt
     2581 gtcggagatg gaaacgtcgc cattgacggt gacggtccag gccgaaccga cgggaacggc
     2641 gaccgagccg tcgatcttca ccgacgtacc gtcagcgttg aacgcattgg tttcggcgtc
     2701 ggtgtagttc accgcagcgc cgaccgaacc aacctgctga gcgaaggcgg gcgccgagac
     2761 ggcgaggacc gcgacagcgg cggcggcgaa aagttgggtc ttcatagtgg gtaatcccct
     2821 tagtgaaaca gccccggcgc tggcggtcgt tgtgggggag gggccgccag tccgccgggg
     2881 agggcggcat ctatggcgcg tcgccccacg ccacaagggg tcatggtccc gctagagggt
     2941 cagttgatgc gcaattgtga cacttgaaag actgaagccg tcgactaatc gcaagcgcgc
     3001 gcactttcct gcccatgtca cagtgttatt cgacaatccc gtgacagatg agggcttgcg
     3061 gaggcggggc tgggcgccta tccagaagcg gctcgccgag tcgcctcatt ttgtgtcgcg
     3121 ccaaggcaag cggagcgctt gacgaaaccg gattcggaac gtacaagcgc gcctctgttg
     3181 gaccggctgt gagctttagg gctcaaacgc ggttcgcgag acgcccggac atagcgccgc
     3241 caaccttctt tcgagaaggg gcgcaatatc tggacataac ccccacgggc gaccgtgagg
     3301 gccatttgtt ttgcggacgc tgcgttcgaa ctctctcgag ggttc
//
LOCUS       DAOMEO010000235         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3237_length_3324_cov_2.501377, whole genome shotgun sequence.
ACCESSION   DAOMEO010000235 DAOMEO010000000
VERSION     DAOMEO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_3324_cov_2.501377"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLH31_06625"
     CDS             <1..748
                     /locus_tag="PLH31_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88929.1"
                     /translation="ALLLALTMMGQAPVAPAATPPSDDYGYVAWCFGALGGYVDLYDQ
                     VMPEVTRIEKAFPGPDGVEASLKTYPEMRAQARTDMKIFTAAITAAERASPRPISEYG
                     GAAVKKGRSVWTGADSANKARLAQVWMSWSPPGDCETRAKALEKRSNILGKALIYNAV
                     KPVEPAPEPTVASMPEPEAAPPVEQAAPPEPVMTPAPSPAIEVETAIASPQASTKAEF
                     VIDAADPKPCPGRLEPGKRDGKDVLVCKPD"
     gene            complement(866..>1783)
                     /locus_tag="PLH31_06630"
     CDS             complement(866..>1783)
                     /locus_tag="PLH31_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HQR88930.1"
                     /translation="ADYADALIDRLGDRLKRYIILNEAAVHTVFGHVTGEHAPGLKNA
                     DLLGPVIHHMNLGQGLAIQALRAGASDLSIGTTMALQPCRPAGGSWALWNRLASDGLD
                     GLWNRAWLDPLFKGAYPKVMDDFLKGGVVRDGDLATLRQKVDFLGVNYYAPAYVRLDL
                     NAPGKISPTPPPQGAELDAFGRHIDPSGLYEVLEMVRRDYGNPPVLITENGCSDPFGQ
                     RPAVIDDQFRIAYLRRHLQAVLAAREAGSPIGGYFAWTLLDNFEWALGYTSKFGLVAH
                     DRATGVRTPKASYGWFKALAASGALPPKP"
     assembly_gap    1784..1793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1794..2297)
                     /locus_tag="PLH31_06635"
     CDS             complement(<1794..2297)
                     /locus_tag="PLH31_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HQR88931.1"
                     /translation="MVLSGGAIGGAALGLSGCDARPDTELDPKSRQFPQDFVWGVASA
                     AFQTEGAQTADGRGPSVWDGFETLPGKVIDGSDARVATDSYRRYAEDIDLIAAAGLDA
                     YRFSISWSRILPSGAGAINPAGLDHYSRMVDALLAKGVTPYATLFHWDLPQGLQDKGG
                     WTNRDTAL"
     gene            complement(2431..>3324)
                     /locus_tag="PLH31_06640"
     CDS             complement(2431..>3324)
                     /locus_tag="PLH31_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HQR88932.1"
                     /translation="AVVSDADQGQAEMLRQADVALYRAKEAGRGRYCFFEPEMDAALR
                     LRKGLERDLRAALDQDRLTVAYQPLTDARGVILGVEALARWHDPERGDISPAIFIPLA
                     ESTGLIAAIGRSVFRRACYDSLRWSGLEMSVNVSALQLAAPGFVDEIEQILLETGAEA
                     SGFVLEITETALLKDSDGVHEALRRIKRLGFRLALDDFGTGYASLAYLRRHPIDKLKI
                     DRSFVSTLSPDGEASSVAGAIISLGVETQGQFDTLVKLGCVEFQGFLLGRPMPADEIA
                     AARDARQELANAAKVTSRPRR"
BASE COUNT          562 a         1153 c         1100 g          499 t
ORIGIN      
        1 cgctcttctt ctggccctga ccatgatggg ccaggccccc gtcgcccccg cggccacgcc
       61 gcccagcgat gactatggct atgtcgcctg gtgctttggc gccctgggcg gctatgtgga
      121 tctgtacgat caggtgatgc cggaagtgac ccgcatcgag aaggccttcc ccggtcccga
      181 tggcgtcgaa gcctccctga agacctatcc cgagatgcgc gcccaggcgc gcaccgacat
      241 gaagatcttc accgcagcga tcaccgccgc cgaaagggcc agcccgcgcc cgatctccga
      301 atatggcggc gcggccgtca agaagggccg ctcggtctgg accggcgcgg attcggccaa
      361 caaggcccgc ctggcccagg tgtggatgag ctggtcgccg cccggcgact gcgagacccg
      421 cgccaaggcg ctggagaagc gctcgaacat cctgggcaag gcgctgatct acaacgcggt
      481 caaacccgtc gagccggcgc ccgagccgac ggtggcctcc atgccggagc cagaggccgc
      541 accgcccgtc gaacaggccg cgccgcccga gcccgtgatg acgccagcgc ccagcccagc
      601 catcgaggtc gagaccgcca tcgcctcgcc ccaggcctcg accaaggctg agtttgtcat
      661 cgacgccgcc gaccccaagc cttgccccgg ccgtctggag ccaggcaagc gcgacggcaa
      721 ggacgtgctg gtctgcaagc cggactagag cgttttccga taagtgtgaa acggttatcg
      781 gatcaaaaaa cgctctaaac ttctgattta gagccctttt cgcccgaccc tgatgaaaca
      841 atcaggtcgg aaagggctct agggactagg gctttggcgg cagcgctccg ctcgccgcca
      901 aagccttgaa ccagccatac gacgccttgg gcgtgcgcac gcccgtcgcc cggtcatgcg
      961 ccaccaggcc aaatttcgag gtgtagccca gggcccactc gaaattgtcc agcagggtcc
     1021 aggcgaaata gccgccgatg ggcgagccgg cctcccgcgc agccagcacc gcctgcagat
     1081 ggcggcgcag ataggcgatg cggaattgat cgtcgatcac cgccggcctc tggccgaagg
     1141 ggtcagagca gccgttctcg gtgatcagca ccggcggatt gccatagtcg cggcgcacca
     1201 tttccagcac ctcatagagg cctgacggat caatgtggcg gccaaaggcg tccagctcgg
     1261 cgccctgggg cggcggcgtc gggctgatct tgccgggcgc gttcaggtcc agccgcacat
     1321 aggctggcgc gtagtagttc acacccagga aatcgacctt ctgccggagg gtcgccaaat
     1381 cgccgtcgcg cacgacgccg cctttcagaa agtcgtccat caccttggga taggcgccct
     1441 tgaacagggg atccagccag gcgcggttcc aaaggccatc cagcccgtcg gaggctagac
     1501 ggttccacag ggcccagctc ccgccggccg ggcggcaagg ttgcagggcc atggtggtgc
     1561 cgatcgaaag atcgctcgcg ccggcccgca gcgcctggat cgccagcccc tgcccgaggt
     1621 tcatgtgatg gatgacggga ccaagcaggt cggcgttctt caggcccggc gcgtgctcgc
     1681 ctgtcacgtg gccaaacacg gtgtgaaccg ccgcctcgtt gagaatgatg tagcgcttga
     1741 gccggtcgcc cagccggtcg atcagggcgt cggcatagtc ggcnnnnnnn nnncagggcg
     1801 gtgtcgcggt tggtccagcc gcccttgtcc tgcaggccct gcggcaggtc ccagtggaac
     1861 agggtggcgt agggcgtcac gcccttggcc agcagggcgt cgaccatgcg cgaatagtgg
     1921 tccagccccg ccggattgat cgcccccgcc ccgctgggca gaatccgcga ccagctgatc
     1981 gagaaccgat aggcgtccag ccccgccgcg gcgatcagat cgatatcctc ggcgtagcgg
     2041 cgatagctgt cagtggcgac acgggcgtcg gagccgtcga tcaccttgcc gggcagcgtc
     2101 tcgaacccgt cccagacgct gggcccgcgc ccgtcggcgg tctgcgcgcc ttcggtctgg
     2161 aaggccgccg aggccacgcc ccagacaaag tcctgcggaa actgccggga cttgggatcc
     2221 agctcggtgt ccggcctggc gtcgcatcct gacaggccaa gagccgcccc gccgatcgcc
     2281 ccaccactga gaaccatggc cgcgaggcct ctccggctaa cgccctgatg atccacgccg
     2341 catccctccc aagctcggag gtcgacgcct ccgttcggcg tgaggatgcc gtggaagaac
     2401 gccggttggc cagccttacc ttacaggccg ctagcggcgc gggcgggacg tgaccttggc
     2461 cgcattggcc agctcttggc gcgcgtcgcg ggcggcggcg atctcgtcgg ccggcatcgg
     2521 gcggcccagc aggaagccct ggaactcgac gcagcccagc ttcaccagag tgtcgaactg
     2581 gccctgggtc tcgacgccca ggctgatgat ggccccggcc accgagcttg cctcgccgtc
     2641 gggcgacagg gtggagacga acgagcggtc gatcttcagc ttgtcgatcg gatggcggcg
     2701 cagataggct aggctggcat agccggtgcc gaagtcgtcc agcgccaggc ggaagcccag
     2761 gcgcttgatg cgccgcaggg cctcgtggac gccgtcgctg tctttcagca gggccgtctc
     2821 ggtgatctcc agcacaaagc ccgaggcctc cgcgccggtt tccagcagga tctgctcgat
     2881 ctcgtcgacg aagcccggcg cggccagctg cagcgccgag acattgaccg acatctcaag
     2941 ccccgaccag cgcaggctgt catagcaggc gcggcgaaac actgaccgtc cgatggccgc
     3001 gatcaggccg gtcgactcgg ccagcgggat gaagatggcc ggcgagatgt cgccccgctc
     3061 gggatcgtgc catcgggcca gggcctcgac gcccaggatc acgccccgcg catcggtcag
     3121 tggctgatag gcgacggtca ggcggtcttg atccagggcg gcccgcaggt cgcgctcaag
     3181 gcccttgcgc aggcgcaggg ccgcgtccat ttccggctcg aagaagcagt aacgaccccg
     3241 gccggcctcc ttggcgcgat acagggccac gtcggcctgg cgcagcatct cggcctggcc
     3301 ctggtcggcg tcggacacca ccgc
//
LOCUS       DAOMEO010000236         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3241_length_3318_cov_2.282869, whole genome shotgun sequence.
ACCESSION   DAOMEO010000236 DAOMEO010000000
VERSION     DAOMEO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_3318_cov_2.282869"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PLH31_06645"
     CDS             complement(<1..1151)
                     /locus_tag="PLH31_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR88933.1"
                     /translation="METLSRLIDRRPPVASTTSCQDVWETFDADPRAFSVAVVDDRKP
                     VGVVYRDVFMGVMAVARDTLQDRPIAEVMDAEPRLVDLDTELSSFVDNLADRPSPVFR
                     TSFVAIDEAGDYAGVGGLNSLLSSYRRRQREANDAMALVERMATDITHHLNGVLAITE
                     RLEQQRLTPDASAFVRAIGDTSRDMGAMLGRAIDLHRSALGQLPMNTTAVRLRDLADA
                     VEARWSERAAEGGSTLLFSYDGDPECGVEVDGERLLQVFDALIESALASGRGVIEACL
                     QARIGADGVHLGGSVRDNATGLAEDRLARIFAPLGAARIEDRTEMALGVGMALAHRIM
                     HGLGGEVKAEVNRGAGLTVRFAFVAPSAETGAAPAAEPSIGPRAAHILIV"
     gene            complement(1351..>3318)
                     /locus_tag="PLH31_06650"
     CDS             complement(1351..>3318)
                     /locus_tag="PLH31_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88934.1"
                     /translation="ARIFLTQGCLPGSLYSNNAYGTVNTSGTLTGELGNIFGFTSGDA
                     NIGDTTDKDLNKFESLFDPIYRSKSDVYQFTLNYDLTENLTVTAMTSYSENSMYSKQD
                     YNRNVSVVPFNTTPFTPGGFFTDPQVGRTNLFTTLDISSNDAEQFSQEFRLSSNFDGP
                     LNFNIGGIHFDYETVTDYYVIGNSLTLSALALNATTPGNGGNPNCNPVTLPATCIGID
                     AGATPDGSGHNYYDNRTPYRLKSDALFGELYWQANDKLKFTVGLRQTRDRKEVDNYKT
                     VLLSPGTGLVLDPVTPTAKVSFSEMTGRFGFDYKPDLSMTDDTLVYAFYSKGYKGGGI
                     NPGVPDNAVGIPQTFDPAFVNAIELGTKNTLLGGSLLLNATGFYYDYQGYQISKIVNR
                     TSVNENVNAKVYGLEFESIWSPVYNLRFNANVGYLHTEIGKGVKSIDTMDRTQGNPAY
                     TVVKVGPSLPNASVGANCVLSSAGVATLLAIGQGGTLPFACGGPAVFSGFLQSRGVPA
                     PTANFLANGTGLYNYGAGYSIEGAAADVSGNELPSSPHWTASIGAQYRWDMPGGWSGT
                     LRGDYYRQTSQYARVYNTEYDRIKAWDNANLTLKIEKSEWGLEIDAFVKNLMNDIPII
                     DAYTSDDSSGLFTNVITQEPRLYGVSVTKKW"
BASE COUNT          582 a          970 c         1151 g          615 t
ORIGIN      
        1 acgatcagga tgtgggctgc gcggggtcct atggacggct cggccgccgg agccgcaccc
       61 gtctcggccg acggcgcgac aaaggcgaag cggacggtca ggccggcgcc ccgattgacc
      121 tcggccttca cctcaccgcc caggccatgc atgatccggt gggccagggc cataccgaca
      181 cccagggcca tctcggtgcg atcctcgatg cgagccgcac ccagcggcgc aaagatccgc
      241 gccaggcgat cctcggccaa tcccgtcgcg ttgtcgcgga cgctgccgcc cagatgaacg
      301 ccgtcggcgc cgatgcgggc ctgcaggcag gcttcgatga cgccccggcc gctcgccaga
      361 gcgctctcga tcagagcgtc gaacacctga agcagacgtt cgccatcgac ctcgacgccg
      421 cattccggat cgccgtcata ggagaacaga agcgtggatc cgccctcggc ggcgcgctct
      481 gaccaacggg cttcgacggc gtcggccaga tcgcgcagcc gcacggcggt cgtgttcatc
      541 ggcagctgac ccagggccga gcggtgcagg tctatggccc ggccgagcat cgcgcccatg
      601 tcgcggctgg tgtcgccaat ggcgcggacg aaggcgctgg cgtcgggggt cagccgctgc
      661 tgttccagac gttcggtgat ggccaggacg ccgttcaggt gatgggtgat gtcggtggcc
      721 atgcgctcga ccagcgccat ggcgtcgttg gcttcgcgtt gacgacggcg ataggacgac
      781 agcaaggagt ttagcccgcc cacacccgca taatcgcctg cctcatcgat ggccacgaac
      841 gaggtgcgga acacgggcga gggccggtcg gcgagattgt cgacaaagct gctgagctcg
      901 gtatcgaggt ccaccagccg cggttcggcg tccatgacct cggcgatcgg gcgatcctgc
      961 agcgtgtcgc gtgcgacagc catgacgccc atgaacacgt cgcggtagac gacgccgacg
     1021 ggcttgcggt cgtcgaccac ggcgaccgaa aaggcgcgcg gatcggcgtc gaatgtctcc
     1081 cacacgtcct ggcaggacgt ggtcgaggcg accggcggac ggcgatcaat aagacgagat
     1141 agcgtttcca tgcggccgct tcgctggcgt ctgtgaaaga ccagattgcc cgcgtcgccc
     1201 ttgcggagtc gttaagtccg ggcagattct cgtgaaggtg gtttcggcga tcgcccttgc
     1261 cgcgccctgc gacagcgatc aggccaaaaa gaaagggcgg cgggatgacc cgccgccctc
     1321 tgcagaaaga cgttcttcag gaacggacgg ctaccacttc ttggtgacgc tcacgccata
     1381 gaggcgcggc tcctgagtga tgacattggt gaacaggccc gaggagtcgt cgctggtgta
     1441 ggcgtcgatg atcgggatgt cgttcatcag gttcttgacg aaagcgtcga tttccagacc
     1501 ccactccgac ttctcgatct tgagggtcag gttggcgtta tcccaggctt tgatgcggtc
     1561 atattcggtg ttgtagaccc gggcgtactg gctcgtctgg cgatagtagt cgccgcgcag
     1621 ggtgcccgac cagccgccgg gcatgtccca gcgatactgg gcgccgatcg aggcggtcca
     1681 gtgcggcgag ctgggcagct cgttgcctga cacgtcggct gccgcgcctt cgatcgagta
     1741 gccggcgcca tagttgtaga ggccggtgcc attggccagg aagttggccg tgggcgccgg
     1801 aacgccgcgg ctctgcagga agccggagaa gaccgccggg ccgccgcagg cgaagggcag
     1861 ggtgccgccc tgaccgatgg ccagcagggt ggcgacgccg gccgagctga gcacgcagtt
     1921 ggcgccgaca ctggcgttgg gcaggctggg gccgaccttg accaccgtat aggctgggtt
     1981 gccctgggtg cggtccatgg tgtcgatgga cttcacgccc ttgccgatct cggtgtgcag
     2041 ataaccgaca ttggcgttga agcggagatt gtagaccggc gaccagatcg actcgaattc
     2101 caggccatag accttggcgt tgacgttctc gttgaccgag gtgcggttga cgatcttcga
     2161 gatctggtag ccctggtagt catagtagaa gcccgtcgca ttgagcagca ggctgccgcc
     2221 cagaagggtg ttcttggtgc ccagttcgat cgcgttgacg aatgccggat cgaaggtctg
     2281 agggatgccg acggcgttgt cgggcacgcc cggattgatg ccgccgccct tgtagccctt
     2341 ggaatagaag gcatagacga gggtgtcgtc ggtcatcgac aggtctggct tgtagtcaaa
     2401 gccaaagcgg ccggtcatct cgctgaagct gaccttggcg gtcggcgtga cgggatcaag
     2461 gaccaggccc gtacccgggc tgagcagcac ggtcttgtag ttgtcgactt ccttgcggtc
     2521 gcgcgtctga cgcaggccga cggtaaactt cagcttgtca ttggcctgcc agtacagttc
     2581 gccaaacagg gcgtccgact tcaggcgata gggcgtacgg ttgtcgtaat agttatggcc
     2641 cgaaccatcc ggcgtggcgc cggcgtcgat gccgatgcag gttgcaggca gagtgaccgg
     2701 attacagttc ggattaccgc cattgccggg ggtcgtggcg ttcagggcca gggccgacag
     2761 ggtcagcgag ttgccgatca cgtaatagtc ggtgacggtt tcatagtcga aatggatgcc
     2821 gccgatgttg aagttcagcg ggccatcaaa gttcgacgag aggcggaatt cctggctgaa
     2881 ctgctcggcg tcattggacg agatgtccag ggtggtgaac aggttcgtcc ggccgacctg
     2941 ggggtcggtg aagaagccgc ccggggtgaa gggcgtggta ttgaacggca caaccgagac
     3001 gttccggttg tagtcctgct tggaatacat cgagttttcg ctgtacgacg tcatcgccgt
     3061 cacggtcagg ttttcggtca ggtcgtagtt cagggtgaac tgatagacgt cggacttgga
     3121 ccgatagatc gggtcgaaca gcgactcgaa cttgttgagg tccttgtcgg tggtgtcgcc
     3181 gatgttggcg tcaccgctgg tgaagccaaa gatgttgccc agttcgcccg tcagggtgcc
     3241 tgaggtgttg acggtcccgt aggcgttgtt gctgtacagc gagccgggca ggcagccctg
     3301 cgtcaggaag atgcgcgc
//
LOCUS       DAOMEO010000237         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3249_length_3313_cov_1.812462, whole genome shotgun sequence.
ACCESSION   DAOMEO010000237 DAOMEO010000000
VERSION     DAOMEO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3249_length_3313_cov_1.812462"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLH31_06655"
     CDS             <1..268
                     /locus_tag="PLH31_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR88935.1"
                     /translation="LDYTLARAFAYGQGPSAHCAAASPPDRIGVARWMVIGVAHAAVF
                     GAVAGLKWLTRSQDRANWLDKAARGLGKTLWWGPFKIHFYGRTA"
     gene            154..1185
                     /locus_tag="PLH31_06660"
     CDS             154..1185
                     /locus_tag="PLH31_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88936.1"
                     /translation="MADPFAGPRQLAGQGRARSGQDPVVGPLQDPFLREDRVSVITDW
                     TPEKARAFGADNLAFEHGLHERPMFDDEGLARLLDLYPRDQLGVFTMGEDPKAWTTWR
                     KGAAGNLTGDQLLDAAQSGRIWLNLRHTNDYVPEYAALGDEIFAEKEAQVPGLRTFKR
                     DLGMLISSANAQVFYHLDVPLVSLWQLRGQKRVWVYPVADPYVGELDLEKIVLRETAE
                     QFAFDPEWDKGAAVYDLTPGKMVTWRQNAPHRIENGPMLNVSLSIEFMTPPALMRANV
                     IYANGVLRRRLGARPRIQNGFGPAALTKLAVARGAKALNLQTPHVRHLPVTFGLDASK
                     PGVLIESRA"
     gene            1224..1748
                     /locus_tag="PLH31_06665"
     CDS             1224..1748
                     /locus_tag="PLH31_06665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQR88937.1"
                     /translation="MSQAVTIELGEGAEHDARALRNAFGCFTTGVTVVTTISADGRKV
                     GLTANSFTSVSLDPPLALICVDLKSSSLPSLDAAGVFAVNVLHAEHQELARNFVRKDI
                     DRFAGIETETWRTGAPILPGCMANFECETHHVFDAGDHRVYVGRVVKLRYDPDHEPLV
                     YLQGRFRRVHVDAE"
     gene            complement(1756..>2162)
                     /locus_tag="PLH31_06670"
     CDS             complement(1756..>2162)
                     /locus_tag="PLH31_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQR88938.1"
                     /translation="LSHLRALPVDTARHELQSLPGVGVKVAACVLNFSDLAMRALVVD
                     THVHRVARRIGLVSSGDTTQAYHALMGMAPEAWTADDLFELHWLMKRGLGQMLCPHEG
                     PRCGACPLKHDCAKIGVGHSADVLAWRPRGVD"
     assembly_gap    2163..2172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2173..2480)
                     /locus_tag="PLH31_06675"
                     /pseudo
     CDS             complement(2173..2480)
                     /locus_tag="PLH31_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517728.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(2544..3107)
                     /locus_tag="PLH31_06680"
     CDS             complement(2544..3107)
                     /locus_tag="PLH31_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR88939.1"
                     /translation="MTEKPVKRATAKADDRRRQVAALPWRGAGQSLQILLVSSRETKR
                     WVIPKGWPMKGRSDPAAAAQEAYEEAGLDGVIAEQSIGDYEYLKRLKSGVARLVRVDV
                     YPFQVTGGHAVWPEKGQRVLQWMSPIEAALGVQEPELRELIARFAGVDLPDEERPAPA
                     RAPTPVRVALQRLRRRVTALWGRARRP"
     gene            complement(3104..>3313)
                     /locus_tag="PLH31_06685"
     CDS             complement(3104..>3313)
                     /locus_tag="PLH31_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88940.1"
                     /translation="DVEGLRMTPVEWAREREPGMRLQGLSAGQARRALVTHRSLPVST
                     TRRITGAMIGWRRETARRRIGMLKT"
BASE COUNT          535 a         1110 c         1098 g          560 t
ORIGIN      
        1 cctcgactac accctggccc gcgccttcgc ctatggccag ggtccttcag cccactgcgc
       61 cgccgccagc ccgcctgacc ggatcggcgt ggcgcgctgg atggtcatcg gcgtcgcgca
      121 cgctgcggta ttcggcgccg tggccggcct gaaatggctg acccgttcgc aggaccgcgc
      181 caactggctg gacaaggccg cgcgcggtct gggcaagacc ctgtggtggg gccccttcaa
      241 gatccatttc tacgggagga ccgcgtgagc gtgatcaccg actggacgcc tgaaaaggcc
      301 agggcctttg gcgccgacaa cctggccttc gagcacgggc tgcacgagcg cccgatgttc
      361 gacgacgaag gtctggcgcg actgctggat ctgtatcccc gcgaccagct gggcgtcttc
      421 accatgggcg aggaccccaa ggcctggacc acctggcgca agggcgcggc gggaaacctg
      481 accggcgacc agttgctgga cgccgcccag agcggccgca tctggctgaa cctgcgtcac
      541 accaacgatt atgtgcctga atatgccgcg ctcggtgacg agatcttcgc cgagaaggag
      601 gctcaggttc cgggtctgcg cactttcaag cgcgacctgg gcatgctgat cagctcggcc
      661 aacgcccagg tgttctatca cctcgatgta ccgctggtgt cgctgtggca gctgcgcggc
      721 cagaagaggg tctgggtcta tccggtcgcc gatccctatg tgggcgagct ggacctggag
      781 aagatcgtgc tgcgcgagac cgccgagcag ttcgcctttg atcccgaatg ggacaagggc
      841 gcggcggttt atgacctgac gccgggcaag atggtcacct ggcggcagaa cgcgccgcac
      901 cggatcgaga acggtccgat gctgaacgtc tcgctgtcga tcgaattcat gaccccgccg
      961 gccctgatgc gggccaatgt gatctacgcc aatggcgtgc tgcgtcgtcg cctgggcgcc
     1021 aggccgcgca tccagaacgg gtttggcccg gcggccctga ccaagctggc cgtggcgcgc
     1081 ggcgccaagg cgctgaacct gcagacgccc catgtgcgtc atctgcccgt gacctttggc
     1141 ctggacgcca gcaagccggg cgttctgatc gaaagccgcg cctgacgctt gctcccgggc
     1201 ccgcttcatc gcagaagtcg ccgatgagtc aggcagtcac gattgaactg ggcgagggcg
     1261 ccgaacacga cgcccgggcg ttgcggaatg cgttcgggtg tttcaccacc ggcgtcacgg
     1321 ttgtcaccac catcagcgct gacgggcgca aggtggggct gacggccaat tccttcacct
     1381 cggtgtcgct ggatccgccc ctggcgctga tctgcgtcga cctgaagtcc agcagcttgc
     1441 cctcgctgga cgcggccggt gtgttcgcgg tcaacgtgct gcatgccgaa caccaggaac
     1501 tggcccgcaa cttcgtgcgc aaggacatcg accggtttgc cgggatcgag accgagacct
     1561 ggcgcaccgg cgcgccgatc ctgcccggtt gcatggccaa tttcgaatgc gagacccatc
     1621 acgttttcga cgcgggcgat caccgcgtgt atgtcggccg ggtggtgaag ctgcgctacg
     1681 atcccgatca tgagccgctg gtctatctgc agggccgctt ccggcgggtc cacgtcgacg
     1741 ccgaataggg tcggttcaat cgacgccgcg cggtctccag gccaggacat cggcgctatg
     1801 gccgacgccg atcttggcgc agtcatgctt cagcgggcag gcgccgcagc gcggcccttc
     1861 gtgcgggcac agcatctggc ccaggccgcg cttcatcaac cagtgcagtt cgaacaggtc
     1921 gtccgccgtc cacgcttccg gcgccatgcc catcagggcg tgatacgcct gtgtggtatc
     1981 gccggagctg accaggccga tccgccgcgc gacgcggtgg acatgggtgt cgaccacgag
     2041 cgcgcgcatg gccaggtcgc tgaagttcag gacacaggcg gcgaccttga cgccgacgcc
     2101 cggtaggctc tgcagttcgt gccgcgccgt atcgacgggc agggcgcgca gatggctgag
     2161 cgnnnnnnnn nngcctggat catccgcaag gccatcggca ggcgacgggc cttgtcgtga
     2221 gcgtgggtga cgtcgtggat gatttccaac acctgggcgt ccgaagcctg ggccagatct
     2281 tcccagcact tgaacgcggc ccgcaggcgc aggaacgccg cccaggagac cgcgtcgggc
     2341 gtccggccgc tgatcgagga tttcaccagc tgcgagatgg ggtccatccg cgccacgggt
     2401 cgctgagctc caaattcgca gagcagggcg tcgcgaacgc tttcgagcgg cgagcgtgcg
     2461 agggttaggg aaagctgcat gggatctcgc gccagaacat aataggaaca ttatgcggcg
     2521 cgcagcgaat tgtccacagg ctgttatggg cgccgggctc ggccccacag cgcggtgacc
     2581 cggcgacgca ggcgttgaag ggccacccga acaggcgtcg gcgcacgagc gggcgcggga
     2641 cgttcttcgt ctggcaagtc cacacccgcg aagcgggcga tcagttcgcg cagttcaggc
     2701 tcctgcacgc cgagcgcggc ctcgatgggg ctcatccatt gcagcacccg ctggcccttt
     2761 tctggccaga cagcgtgccc gcccgtgacc tgaaaagggt agacgtcgac cctgaccagc
     2821 cgcgccacgc cgcttttcag gcgcttgaga tattcatagt cgccgatgga ctgctcggcg
     2881 atcacgccat ccaggccggc ctcctcatag gcttcctggg ccgccgccgc gggatcgctg
     2941 cgccccttca tcggccagcc cttggggatg acccagcgct tggtttcacg cgacgagacc
     3001 agcaggatct ggagagactg gccggccccg cgccatggca gggcggcgac ctgacgacgc
     3061 ctgtcgtcgg ccttggccgt tgcccgcttg accggcttct ccgtcacgtc ttcagcatcc
     3121 cgatgcgtcg tctcgccgtc tcgcgacgcc agccgatcat agcgcccgtg atccggcgag
     3181 tcgtcgagac cggaaggctg cgatgcgtca ctaaggcgcg ccttgcctgg ccagcgctca
     3241 atccttgaag ccgcatcccc ggttcccgtt cccgcgccca ttcgacgggc gtcatgcgaa
     3301 gtccttcgac atc
//
LOCUS       DAOMEO010000238         3295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3270_length_3295_cov_2.529321, whole genome shotgun sequence.
ACCESSION   DAOMEO010000238 DAOMEO010000000
VERSION     DAOMEO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_3295_cov_2.529321"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLH31_06690"
     CDS             <1..607
                     /locus_tag="PLH31_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQR88941.1"
                     /translation="ADAVTVSTADVALAPGARFAQTILTTGARRQRLETRVAHPGAHA
                     ELRLDGIYLLDKQRHADLTTQVTHGGVDGTTSQLTKGMVDDQARGVFQGRIIVQPGAD
                     QTDARMGHHALIMSDKAEVDAKLELLIFADDVSCAHGNTIGALDEQAIFYARQRGIPE
                     LEARAMLTEAFVGEVVERIEHEPARQLARAWVAQHLGRSIL"
     gene            607..1833
                     /locus_tag="PLH31_06695"
     CDS             607..1833
                     /locus_tag="PLH31_06695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQR88942.1"
                     /translation="MAFDAPFDVEAIRAQFPILQRMVHGKPLIYLDTAASAQKPDAVL
                     DAMMGLARTSYANVHRGLHTLANETTEAYEKARETVARFINAEPSEIAWTKSATEAVN
                     LVASSFGLSLNAGDEIVISEMEHHANIVPWHFLRERKGVVLKFVPVTDDGRLDMEAYK
                     GLLSERTRMVALTHMSNVLGTVNDVAEIVRLAHAAGAPVLLDGCQGVVHTKVDVKAMD
                     VDFYVFSGHKLYGPTGIGVLYGKAERLAALPPYQGGGEMIGSVSMDTVTYADPPHRFE
                     AGTPAILEAVGLGAAIEWLNGLDRDAAFAHEHALYERVAERLRGVNGLRILGEAPGKG
                     AVLSFTVEGAHAHDIAQVLDRYGVAVRAGTHCAEPLMRRFGVTSSARASFALYNTEAE
                     ADAFVDALAKARSFFS"
     gene            1841..2197
                     /locus_tag="PLH31_06700"
     CDS             1841..2197
                     /locus_tag="PLH31_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HQR88943.1"
                     /translation="MTDAAVPETVSALSKEELAKLTDLLIEKLKSVYDPEIPVDIYEL
                     GLIYKVDVSDEKHVAIDMTLTAPGCPVAGEMPGWVKDAVMEIPGLKSCTVDLVFEPPW
                     DASRMSDEAKLQLNMF"
     gene            2212..>2285
                     /locus_tag="PLH31_06705"
     CDS             2212..>2285
                     /locus_tag="PLH31_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeS assembly scaffold SufA"
                     /protein_id="HQR88944.1"
                     /translation="MDIAVTPRARRPRPKVVTLTDAAAA"
     assembly_gap    2286..2295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2296..2631
                     /locus_tag="PLH31_06710"
     CDS             <2296..2631
                     /locus_tag="PLH31_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQR88945.1"
                     /translation="ARVKEIMARKSLEEPGKPYVGLRVGVKNGGCAGSEYVFEYAAER
                     NPLDEVVEDKDVTILIDPKAVLFLIGTTIDYETTKLSSKFVFNNPNETDACGCGESVT
                     IQPAAQALD"
     gene            2921..>3295
                     /locus_tag="PLH31_06715"
     CDS             2921..>3295
                     /locus_tag="PLH31_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HQR88946.1"
                     /translation="MVKTILVYSMGEVIGDGLIKLPFIAGLRAAFPDARITWCAAKGQ
                     TVYAGPLKAVVEGYIDEIITVGVTGAKLLDILPWVKPFSGRRFDLVIDTQENLRRSAV
                     VRRAAGVLVSAAALARTTDWPVA"
BASE COUNT          572 a         1102 c         1061 g          550 t
ORIGIN      
        1 ggccgatgcc gtgacggtga gcacggccga tgtcgccctg gcgcccggcg cgaggtttgc
       61 ccagaccatc ctgaccacgg gcgcgcgtcg tcagcgcctt gagacccgcg tcgcccatcc
      121 cggggcccac gccgaactgc ggctcgacgg gatttatctg ctcgacaagc agcgccacgc
      181 cgacctgacc acccaggtca cccacggcgg cgtcgatggg acgacctcgc aactgaccaa
      241 gggcatggtc gacgatcagg cgcgcggcgt gttccagggc cggatcatcg tccagcccgg
      301 cgccgaccag accgacgccc gcatgggcca tcacgccctg atcatgtcgg acaaggccga
      361 agtcgacgcc aagctcgagc tgctgatctt tgccgacgac gtctcctgcg cccacggcaa
      421 caccatcggc gccctcgacg agcaggcgat cttctacgcc cgccagcgcg gaatccccga
      481 actggaagcc cgcgccatgc tgaccgaggc cttcgtcggc gaggtggttg agcgcattga
      541 gcacgagcct gcccgccagc tcgcccgcgc ctgggtggct cagcatctcg ggcggagtat
      601 tctttgatgg ctttcgatgc gcccttcgac gtcgaggcga tccgcgccca gttcccgatc
      661 ctgcaacgga tggtgcatgg caagccgctg atctatctcg acaccgccgc cagcgcccag
      721 aagcctgacg ccgtgctgga cgccatgatg ggcctggccc gcacctccta cgccaatgtc
      781 catcgcggcc tgcacacgct ggcgaacgaa acgacggaag cctacgaaaa ggctcgtgaa
      841 acggtcgcgc gcttcatcaa tgcagagccg agcgaaatcg cgtggaccaa gagcgcgacc
      901 gaggcggtga acctggtcgc atcgtcgttt ggcctgtcgc tgaacgctgg cgacgagatc
      961 gtgatctccg agatggagca ccacgccaac atcgtgccgt ggcacttcct gcgcgagcgc
     1021 aagggcgtgg ttctgaagtt cgtgccggtc accgacgacg gtcgcctcga catggaggcg
     1081 tacaagggcc tgctgtccga gcgcaccagg atggtcgccc tgacccacat gtcgaacgta
     1141 ctgggcaccg tcaatgacgt ggccgagatc gtccgcctgg cccatgcggc cggcgcgccg
     1201 gtgctgctgg atggctgcca gggcgtggtc cataccaagg tcgacgtaaa ggccatggac
     1261 gtcgatttct acgtcttctc cggccacaag ctctatggcc ccaccggcat cggcgtgctg
     1321 tacggcaagg ccgagcgtct ggcggccctg ccgccctatc agggcggcgg cgaaatgatc
     1381 ggctcggtgt cgatggacac cgtcacctac gccgatccgc cccatcgctt cgaggcgggc
     1441 accccggcga tcctcgaagc cgtcggcctg ggcgcggcga tcgagtggct gaacggcctg
     1501 gaccgcgacg ccgccttcgc ccacgagcac gccctctatg aacgggtcgc cgagcggctg
     1561 cgcggcgtca acgggctgcg gatcctcggc gaggcgcccg gcaagggcgc ggtgctgtcg
     1621 tttacggtcg agggcgcaca cgcccacgac atcgcccagg tgctggaccg ctacggcgtc
     1681 gccgttcgcg cggggaccca ctgcgccgag cccctgatgc gacgctttgg cgtcacgtcg
     1741 agcgctcgcg cctccttcgc cctatataac accgaggctg aagccgatgc gttcgtggac
     1801 gcactggcca aggcccgcag tttcttcagt tgagcgctaa atgaccgacg ccgccgttcc
     1861 cgagactgtt tcggcccttt ccaaggaaga gctggccaag ctgaccgatc tgctgatcga
     1921 gaagctgaag tcggtctatg acccggaaat cccggtcgac atctacgagc tgggcctgat
     1981 ctacaaggtc gatgtctccg acgaaaagca cgtggccatc gacatgaccc tgaccgctcc
     2041 cggttgtccg gtggcgggcg agatgcccgg ctgggtcaag gacgcggtca tggagatccc
     2101 gggtctgaag tcctgcaccg tcgatctggt gttcgagccg ccgtgggacg cctcacggat
     2161 gagcgacgaa gccaagctgc aactgaacat gttctgatcg gttcgaacgc catggacatc
     2221 gccgtcacgc cccgcgcccg tcgcccccgt cccaaggtcg tcaccttgac cgacgccgcg
     2281 gctgcnnnnn nnnnngcacg cgtgaaggag atcatggccc gcaagtcgct tgaagagccg
     2341 ggcaagccct atgtgggcct gcgagtcggc gtgaagaacg gcggctgcgc cggttcggaa
     2401 tatgtcttcg agtacgccgc cgagaggaac ccgctcgatg aggtggtcga ggacaaggac
     2461 gtcaccattc tgatcgaccc caaggcggtg ctgttcctga tcggcacgac gatagactac
     2521 gagaccacca agctgtcgtc gaagttcgtg ttcaacaacc ccaacgaaac cgacgcctgc
     2581 ggctgcggcg agagcgtgac gatccagccc gctgctcagg ctctggactg atgttggccc
     2641 gcacacccaa ggcagtctgc ctggaatgcg gcttgccatg gtcttcgccg gccttcgtcg
     2701 cccatgccgg catccgcgag cacgggcccg cctattagtc ggatcgccgc atcctctgct
     2761 cggtgggctg cgcgtccgag catttccgga aacgcagggc cgatcgaaca tttatcgcca
     2821 taccgttgga ctgtccgatc gacgtccagg gatagatcga gccgcctctc agcggcctcg
     2881 aggcgttgac cggggccggc aggacggcct aatccaggcc atggtcaaaa ccatactggt
     2941 ctattcgatg ggtgaggtga tcggcgacgg gctgatcaag ctgcccttca tcgctggcct
     3001 gcgcgcggcc tttcctgacg cccggattac gtggtgcgcg gccaagggcc agacggtcta
     3061 tgcgggaccg ctgaaggctg tggtcgaagg ctatatcgat gagatcatca cggtgggcgt
     3121 cacgggcgcc aagctgctcg acatcctgcc ctgggtaaag ccgttcagcg gacgcaggtt
     3181 cgatctcgtg atcgacaccc aggagaacct gcgacgcagc gccgtggtca ggcgggcggc
     3241 tggcgttctc gtctcggccg cagcgctggc gcggacgacg gattggccgg ttgcc
//
LOCUS       DAOMEO010000239         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3279_length_3292_cov_2.226753, whole genome shotgun sequence.
ACCESSION   DAOMEO010000239 DAOMEO010000000
VERSION     DAOMEO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3279_length_3292_cov_2.226753"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PLH31_06720"
     CDS             complement(<1..1075)
                     /locus_tag="PLH31_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HQR88947.1"
                     /translation="MRQTFDESTDPSFGPKHVPLIRAAMARQGLDGFLVPHEDEHQNE
                     YLPAANDRLAWASGFTGSAGAGVILSDRAAVFVDGRYTLQVREQVDQDVFEIRDLVEG
                     GVPAYLETAKPGAVIGYDARLHSPQALDSLKAAAAKAGAQLKPVAVNPLDEAWGAQRP
                     AQPAAPIVPQPVEYAGEDSASKRSRVGASLASLGADAAVITAPASIAWLFNVRGGDVI
                     RSPLPLSQAIINSDGTARVFIDPAKITEELPAWLGNQVSLETPDALDAALADLAGKSV
                     LVDPGQSSAWYFDTLTAAGAKVVRGMDPCTLPRACKNAVEIEGTREAHRRDGAALTRF
                     LHWLATEGQINPPDEKQAVAKLEG"
     gene            complement(1118..1543)
                     /locus_tag="PLH31_06725"
     CDS             complement(1118..1543)
                     /locus_tag="PLH31_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88948.1"
                     /translation="MTYTDPQIPPTERVIEVRERNTTGWVVAGIVAIVAVIAVTFMLT
                     QRSSETTQDELLQAQEQGRAVGVIEGAQSSVASSAQQAQFAAENAARNASAAADEARR
                     ETERAAANASQAAQDAADRVAASGDTAPSVDAPPASSPQ"
     gene            complement(1629..>1965)
                     /locus_tag="PLH31_06730"
     CDS             complement(1629..>1965)
                     /locus_tag="PLH31_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQR88949.1"
                     /translation="TDIDRPSVRIARENALVNQANAKFVHASGLGHRLVADNAPYDLV
                     FANILARPLISLAQDIKRALVPGGTVILSGLLRTQERMVKAAYLSRGFKVVTRIHRDA
                     WATLVLQRP"
     assembly_gap    1966..1975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1976..2463)
                     /locus_tag="PLH31_06735"
     CDS             complement(<1976..2463)
                     /locus_tag="PLH31_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQR88950.1"
                     /translation="MTDYTPQQIVARGARADAEAAAEAIDADGALEGATYSILEEDED
                     KNILARVLAGFKVKVTTEVLADADWLAMALSGLPPVRAGRFFVYGMHDRGRLPASTVN
                     LRIEAGAAFGTGHHGTTVGCLQAYDRLIKARTFKKVLDVGAGTGLLAIAAARTGSKLA
                     VGT"
     gene            complement(2515..2994)
                     /locus_tag="PLH31_06740"
     CDS             complement(2515..2994)
                     /locus_tag="PLH31_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88951.1"
                     /translation="MDIDLTPLKTFTASRPVMYGAPAAALLGVVLGLFFQVGSQAEPA
                     DDAQTPSRAYVEEEANPIAWPSGKVPDYVIGTDFLAEAKPYDHPPAVYADDDYAAQYV
                     PASTPAVEAAPPPVVIAARPAYENRPEREESRWASTEGDILDVRLPEDTSMETALLN"
BASE COUNT          475 a         1056 c         1192 g          559 t
ORIGIN      
        1 agccctccag cttggcgacg gcttgcttct cgtcgggcgg attgatctga ccttcggtgg
       61 ccagccagtg caggaagcgc gtcagggccg cgccgtcgcg gcggtgcgcc tcgcgcgtgc
      121 cctcgatctc gacggcgttc ttgcaggccc ggggcagggt gcaaggatcc atgccccgca
      181 ccaccttggc gcccgccgcc gtcagcgtgt cgaagtacca ggccgaggac tggccgggat
      241 cgaccagcac gctcttgcca gccaggtcgg ccagggcggc gtccagcgcg tccggggttt
      301 ccagcgacac ctggttgcca agccaggcgg gcagttcttc ggtgatcttg gccgggtcga
      361 tgaacacccg cgccgtgcca tcgctgttga tgatggcctg agacagcggc aggggcgagc
      421 ggatgacgtc gccgccacgc acattgaaca gccaggcgat cgaggccgga gcggtgatca
      481 cggcggcgtc ggcgccgagg ctggccaggc tggcgcccac ccggctgcgc ttggaggcgg
      541 agtcctcgcc ggcatactcg accggctgag ggacgatggg ggcggccggt tgggcgggac
      601 gctgtgcgcc ccaggcttca tccagaggat tgacggcgac gggcttcagc tgcgcgccgg
      661 ccttggcggc ggcggccttc aggctgtcga gggcctgcgg gctgtgcaga cgggcgtcgt
      721 agccgatgac ggcgccaggc ttggccgttt ccagataggc gggaacgccg ccctcgacca
      781 gatcacggat ttcgaacacg tcctgatcga cctgttcgcg cacctgcagg gtgtagcggc
      841 catcgacgaa gacggccgcg cggtcgctca ggatcacgcc ggccccggcg ctgccggtga
      901 agccgctggc ccaggccagg cgatcattgg cggcgggcag gtattcgttc tgatgttcat
      961 cctcgtgcgg cacgaggaag ccgtccagac cctgtcgcgc catggcggcg cggatcaggg
     1021 gaacgtgctt ggggccgaag gacgggtcgg tggattcatc gaaggtctgg cgcatgcccc
     1081 gatgtaatcc gccgacccga gcctgggaag aggatgatta ttgcggcgaa gaggcgggcg
     1141 gggcgtcgac cgacggggcc gtgtcgccgc tggccgcgac ccggtcggcg gcgtcctggg
     1201 ccgcctggct ggcgttggct gcagcgcgct cggtctcccg gcgggcctcg tcagcggcgg
     1261 cgctggcgtt gcgcgcggcg ttctcggccg cgaactgggc ctgctgggcg ctggaggcga
     1321 cgctggactg ggcgccctcg atcacgccca cggcgcggcc ttgctcctga gcctgcagca
     1381 gctcgtcctg ggtggtctcg gacgagcgct gggtcagcat gaaggtcacc gcgatgaccg
     1441 cgacgatggc cacgatgccg gccaccaccc agccggtggt gttgcgctcg cgcacctcga
     1501 tcacgcgctc ggtgggcggg atctgcgggt cggtataggt catcgggctg ttccttgagc
     1561 cgggaagtct tggcttggga aacgtcgccc tacgtccagt ggttccgctt ggccccctct
     1621 gcgtggcttt agggccgttg cagaaccagc gtcgcccagg cgtcgcggtg gatgcgggtg
     1681 accaccttga agccgcgcga caggtaggcg gccttgacca tccgctcctg ggtgcgcagc
     1741 aggcccgaca ggatcaccgt gccgcccggg accagggcgc gcttgatgtc ctgggccagg
     1801 ctgatcagcg gccgggccag gatgttggcg aacactaggt catagggggc gttgtccgcc
     1861 accagccggt ggcccaggcc cgaggcgtgg acaaacttgg cgttggcctg attgaccaag
     1921 gcgttttccc tggcgatgcg aacgctggga cggtcgatgt cggtgnnnnn nnnnngtgcc
     1981 cacggccagc ttgctgcccg tgcgggcggc ggcgatggcc agcaggccgg tgccggcccc
     2041 gacgtcgagc accttcttga acgtgcgggc cttgatcagg cggtcatagg cctgcaggca
     2101 gcccaccgtg gtgccgtgat ggccggtgcc aaaggccgcg ccggcctcga tgcgcagatt
     2161 gacggtgctg gccggcaggc ggccgcgatc gtgcatgcca tagacgaaga accgcccggc
     2221 ccgcaccggc ggcaggcccg acagcgccat ggccagccag tcggcgtcgg ccaggacctc
     2281 ggtggtcacc ttgaccttga agccggccag cacgcgggcc aggatattct tgtcctcgtc
     2341 ctcttccagg atcgagtagg tcgcgccttc cagcgcgccg tcggcgtcga tggcttcagc
     2401 ggcggcttcg gcgtcggcgc gggcgccgcg ggcgacgatc tgttggggcg tgtagtcggt
     2461 catcccgctc cagtaccgat cccggagccg aaaggcgagc ctttggcgac aggatcagtt
     2521 cagaagcgcg gtttccatcg aggtgtcctc gggcaggcgc acatccagaa tgtcgccctc
     2581 cgtcgacgcc cagcggctct cctcgcgctc tggtctgttc tcataggcag gcctggcggc
     2641 gatcacgacg ggcggcggag cggcttccac cgctggcgta gaggcgggga catattgcgc
     2701 ggcataatcg tcgtcggcat agaccgcagg cggatggtca taaggtttgg cctcggccag
     2761 gaaatcggtg ccgatcacat agtccggaac cttgcctgac ggccaggcga tggggttggc
     2821 ctcttcttcg acataggcgc ggctgggcgt ttgcgcgtcg tcggcgggct cggcctggga
     2881 gccaacctga aaaaacagcc ccaagaccac gcccagcaac gccgcggccg gcgcgccata
     2941 catcaccggg cgcgatgcgg tgaaggtctt cagcggcgtt agatcgatat ccatgacctg
     3001 atagcgcttg agcgcgcgtt ccgttcgatc ccggcgcgag aaataccgcc ggccgaccga
     3061 tcggtgctcg ttttgccgtt tgatttgggg ttggctggtc ttcaagcccg gcggttgggc
     3121 gggcttgtcc gtgccgtatc cccggcggat ggcgaagtga agccattaaa atcaatcact
     3181 tccaactttc ttgcacgtct gtatccccgg ctccaaaacc tgagggatac tgctccttgt
     3241 ccccgtcgca gggggagggc gcctggtacc ggcccaaagc ggcgatgggg at
//
LOCUS       DAOMEO010000240         3288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3282_length_3288_cov_2.270028, whole genome shotgun sequence.
ACCESSION   DAOMEO010000240 DAOMEO010000000
VERSION     DAOMEO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3282_length_3288_cov_2.270028"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1119
                     /locus_tag="PLH31_06745"
     CDS             <1..>1119
                     /locus_tag="PLH31_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR88952.1"
                     /translation="REFADGYKFWVPLLAGEHVTDDAGTGFVHTAPGHGQDDYLAWLN
                     NRASFDEANEQVDSDPELQELNYERLDPEIRNTVDPDGAYFPHVPLFAGLKVIETEGK
                     KAGKFGAANGAVMDKLIEAGNLLARGRVEHSYPHSWRSKAPIIFRNTPQWFIRMDQPL
                     ESGKTLRETAVQAIADTAFHPDAGRNRIGSMVESRPDWLVSRQRAWGTPLAMFVDKDS
                     GQPLMDEAVNARILAAIREGGADAWFTRPDAEFLGEAYDAAQYEKVGDILDVWFDSGC
                     THAFTIEGRDDSHWPADLYLEGSDQHRGWFQSSLLEGCGTRGRAPYKAVLTHGFTQDE
                     NGEKMSKSKGNTVEPQTVTKESGAEILRLWAAMVDYSE"
     assembly_gap    1120..1129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1130..2016
                     /locus_tag="PLH31_06750"
     CDS             <1130..2016
                     /locus_tag="PLH31_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR88953.1"
                     /translation="EDQRIGKTILATTADTYRKLRNTTRYLLGALAGFDEAERVTNYD
                     DFPPLEKFILHRLWELDGQVRAAYEAYRFADVTRPLIEFCQGDLSTLFFDIRKDSLYC
                     DKPTELRRRAYRTVLDYVFERLTIWLSPLTSFTMEEAWQTRFPEAGSNSLRVIPETPA
                     QWRNDAEAARWAKVETVMSVVTGALEVERREKRIGSALEAAPVVHVADADLLAAFDGL
                     DAAEVFRTSAATLVAGEGGAFSTDEVKGVSVDPLQAEGNKCARSWRILPEVGTDPSYP
                     ELSLRDAEAVAWWDSQKG"
     gene            2181..2687
                     /gene="lspA"
                     /locus_tag="PLH31_06755"
     CDS             2181..2687
                     /gene="lspA"
                     /locus_tag="PLH31_06755"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQR88954.1"
                     /translation="MTINITRQGWIAYAIAAVTVALDQLSKLWILHGLDLPSKGTVPL
                     PGPVDLTMVWNRGMSFGLLSDHAEWSRWLLTGFSAVVVVALAIWARKATKPLMAIGLG
                     LIIGGAIGNNLIDRVVYGAVADFIDVRDLYFPWIFNIADAGISIGVAFLLLDSFLTGE
                     DKLAHPTE"
     gene            2785..>3288
                     /locus_tag="PLH31_06760"
     CDS             2785..>3288
                     /locus_tag="PLH31_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HQR88955.1"
                     /translation="MSFNRVATLSVLAAVAATGLAGCQSASKALGMSKVVPDEFRVVT
                     KAPLVVPPDYALRPPAPGEPRPQELQPESAARQALIGQSMAAGRSDGEKLLVARAGVD
                     KADPLIRFVVDDETGDLAHKDETFASKVMFWKKDKAAEVAAIATETGANAPMVVDAAA
                     EEARLKAL"
BASE COUNT          578 a         1112 c         1066 g          522 t
ORIGIN      
        1 ttcgcgaatt cgccgatgga tacaaattct gggttcctct tctcgcgggt gaacacgtca
       61 cggacgatgc cggcaccggc tttgtccata ccgcgcccgg ccatggtcaa gacgactacc
      121 ttgcttggct caacaatcgc gcgtccttcg atgaagccaa tgaacaggtc gatagcgacc
      181 cggaactcca agagctcaat tatgagcgtt tggaccctga aatccgtaac accgtcgatc
      241 ccgacggcgc ctatttcccg cacgtgccgc tgttcgcggg cctgaaggtc atcgagaccg
      301 agggcaagaa ggccggcaag ttcggggccg ccaacggcgc ggtgatggac aagctgatcg
      361 aggccgggaa cctgctggcg cgcggccggg tcgagcacag ctatccgcat agctggcgct
      421 cgaaggcccc gatcatcttc cgcaacacgc cccagtggtt catccgcatg gatcagccgc
      481 tggaaagcgg caagaccctg cgcgagaccg ccgtccaggc catcgccgac accgccttcc
      541 accctgacgc cggccgcaac cgcatcggct cgatggtgga aagccgtccc gactggctgg
      601 tcagccgcca gcgcgcctgg ggcacgccgc tggccatgtt cgtcgacaag gacagcggcc
      661 agcccctgat ggacgaggcg gtcaacgccc gcatcctggc ggccatccgc gaaggcggcg
      721 ccgacgcctg gttcacccgc cctgacgccg agttcctggg cgaggcctat gacgcggccc
      781 agtacgagaa ggtcggcgac atcctcgacg tctggttcga ctccggctgc acccacgcct
      841 tcacgatcga gggccgcgac gacagccact ggccggctga cctgtatctg gaaggctccg
      901 accagcatcg cggctggttc cagtcgtcgc ttctggaagg ctgcggcacc cgcggccgcg
      961 cgccctacaa ggcggttctc acccacgggt tcacccagga cgagaacggc gagaagatgt
     1021 ccaagtccaa gggcaacacc gtcgagcccc agaccgtcac caaggaaagc ggcgccgaga
     1081 tcctgcggct gtgggcggcg atggtcgact attcggaagn nnnnnnnnnc ggaagaccag
     1141 cggatcggca agacgatcct ggccaccacc gccgacacct atcgcaagct gcgcaacacc
     1201 acgcgctacc tgctgggcgc cctggccggc ttcgacgagg ccgagcgggt caccaactat
     1261 gacgacttcc cgccgctgga gaagttcatc ctgcaccgcc tgtgggagct ggatggtcag
     1321 gtccgcgccg cctacgaggc ctatcgcttc gccgacgtca cccggccgct gatcgagttc
     1381 tgccagggcg acctgtcgac cctgttcttc gacatccgca aggacagcct gtactgcgac
     1441 aagcccacgg aactgcgccg ccgggcctat cgcaccgtgc tggactatgt gttcgagcgc
     1501 ctgaccatct ggctgtcgcc cctgaccagc ttcaccatgg aagaggcctg gcagacgcgc
     1561 ttccccgagg cgggcagcaa cagcctgcgg gtgatcccgg agacgccggc ccagtggcgc
     1621 aacgacgccg aggccgcgcg ctgggccaag gtcgagacgg tgatgtcggt ggtgaccggc
     1681 gccctggaag ttgagcgtcg tgaaaagcgg attggctcgg cgctggaggc tgcgccggtg
     1741 gtgcatgtcg ccgacgctga cctgctggcc gccttcgacg gcctcgacgc cgccgaggtg
     1801 ttccgcacca gcgccgcgac gctggtggct ggcgaaggcg gcgcgttctc gaccgacgag
     1861 gtcaagggcg tgtcggtgga tccgctccag gccgaaggta acaaatgcgc ccgctcctgg
     1921 cggatcctgc ccgaagtcgg aaccgatccc agctatccgg agcttagcct tcgtgacgcg
     1981 gaagctgtgg cgtggtggga tagccagaag gggtgacccc cttccccctt gatgggggaa
     2041 gggttgggga tgggggtgac gcggcggtga gactggatcg aagtccccgc ctaattgccg
     2101 ccgccgcatc acccccaccc tgccctcccc catcaagggg gagggtagag acctaattga
     2161 agagccttgg ggctgagaga atgacgatca acatcacccg ccaaggctgg atcgcctacg
     2221 ccatcgccgc cgtgacggtg gcgctcgatc agctcagcaa gctgtggatc cttcacggcc
     2281 tggacctgcc gtccaagggc acagtccccc tgcccggtcc ggtcgatctg accatggtct
     2341 ggaacagggg catgagcttt ggcctgctga gcgaccacgc cgagtggagc cgctggctgc
     2401 tgaccggctt ttcggcggtc gtggtcgtgg ccctggcgat ctgggcgcgc aaggcgacca
     2461 agccgctgat ggccatcggc ctgggcctga tcatcggcgg cgcaatcggc aacaacctga
     2521 tcgaccgggt ggtctatggc gcggtcgccg acttcatcga cgtgcgcgac ctttatttcc
     2581 cgtggatctt caatatcgcc gacgccggga tttcgatcgg cgtggccttc ctgctgctcg
     2641 acagcttcct gacgggcgag gacaaactgg cccatccgac cgaataaggc cgcaatcgcg
     2701 agagagattg gcgcccgcgc catgatgtcg tatcgagagc gctcgaaatt ttggcccggg
     2761 gcgggccacg tggagcatct gttcatgagt ttcaatcggg tcgcgaccct gagcgttctg
     2821 gccgccgtgg ccgccacggg cctcgccggt tgccaatcgg cctccaaggc tctgggcatg
     2881 agcaaggtgg ttcccgacga attccgcgtc gtcaccaagg ccccgctggt ggtgccaccc
     2941 gactatgccc tgcgtccgcc ggcccccggc gagccccgcc cccaggagct gcagcctgaa
     3001 agcgccgccc gtcaggccct gatcggccag agcatggccg ccggccgctc ggacggcgag
     3061 aagctgctgg tggcccgcgc cggcgtcgac aaggctgatc cgctgatccg cttcgtcgtc
     3121 gacgacgaaa ccggtgacct ggcccacaag gacgaaacct tcgccagcaa ggtgatgttc
     3181 tggaaaaagg acaaggcggc cgaggtcgcc gccatcgcca ccgagaccgg cgccaacgcc
     3241 ccgatggtgg tcgacgccgc cgccgaagaa gcccgcctga aggccctg
//
LOCUS       DAOMEO010000241         3285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3285_length_3285_cov_2.327245, whole genome shotgun sequence.
ACCESSION   DAOMEO010000241 DAOMEO010000000
VERSION     DAOMEO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_3285_cov_2.327245"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1186)
                     /locus_tag="PLH31_06765"
     CDS             complement(59..1186)
                     /locus_tag="PLH31_06765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR88956.1"
                     /translation="MTVDITGVCPDRFSRVRDVFEANFADGGELGARFAFAVEGEIVV
                     DLMGGHADRKRTIAFGPDTLTALFSTTKAVAALMVARLVDEGRLAYDQPVADVWPEFA
                     QAGKQDVTVEQALSHQAGLSGFPDETDPAIWFDWDSTCAKLAAMAPLFPVGSASGYHP
                     VTFGYLAGEIFRRVDGRTMGTALREDIAQPFGLDLWIGLPDSEHGRTAELMRPTALPK
                     FGAMNAAVKAAFMTPWAAPSGRGTDEWRRVEIPSANGHATAPALARLMGALANGGMLD
                     GRSILSPAGIKAATFERIVGRDLVLPYDISWGAGFMRNTPNFYYGPTAEAFGHSGWGG
                     SCAFADPTTGVSGSYVMNRQGNALIGDPRAVRLIEAAYASL"
     gene            complement(1199..>1404)
                     /locus_tag="PLH31_06770"
     CDS             complement(1199..>1404)
                     /locus_tag="PLH31_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine kinase"
                     /protein_id="HQR88957.1"
                     /translation="TGDLLTALYAASILEGQTLSYGLARAFGGVAETVTAANIWNAPE
                     LPIVAMGARIKQTSPTVRIERLA"
     assembly_gap    1405..1414
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1415..>1744)
                     /locus_tag="PLH31_06775"
     CDS             complement(<1415..>1744)
                     /locus_tag="PLH31_06775"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HQR88958.1"
                     /translation="AVREAPREGAASRRPSIIIDPTMGDAGKGLYVPAEVADAIAAEL
                     IPRADVVACNAWELQKLTGADARDPQSAMRAARLLGKPTLVSSVHRGSEIGVVLADSK
                     EAWLAAHA"
     assembly_gap    1745..1754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1755..2039)
                     /locus_tag="PLH31_06780"
     CDS             complement(<1755..2039)
                     /locus_tag="PLH31_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine kinase"
                     /protein_id="HQR88959.1"
                     /translation="MPLALIISSHVAGSRVGASAQATALVQFRIDSMVAPTVLFGRHP
                     GWGVPGGAPVPIEVLEGMLDGMEANGLFGLTDIVITGYFASAAQVRAAARA"
     gene            complement(2106..2243)
                     /locus_tag="PLH31_06785"
     CDS             complement(2106..2243)
                     /locus_tag="PLH31_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88960.1"
                     /translation="MQLPLSVRGEEPAAWPSIIAEQIGLARTRAIVRTDASEASSPPL
                     S"
     gene            complement(2318..>2719)
                     /locus_tag="PLH31_06790"
     CDS             complement(2318..>2719)
                     /locus_tag="PLH31_06790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR88961.1"
                     /translation="KVDPRIRKDLTNRLMAVSDAHNVLVAESWAGADLGAIVRQALAP
                     HDHEGVSRFQIDGPLVWLSPHQAVSMALALHELATNALKYGALSAEAGQVEITWNLAH
                     TQLGARQLSLLWRELGGRPLRRRTAPDLARD"
     assembly_gap    2720..2729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2730..2816)
                     /locus_tag="PLH31_06795"
                     /pseudo
     CDS             complement(2730..2816)
                     /locus_tag="PLH31_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
     gene            complement(2841..3209)
                     /locus_tag="PLH31_06800"
                     /pseudo
     CDS             complement(2841..3209)
                     /locus_tag="PLH31_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
BASE COUNT          524 a         1068 c         1153 g          510 t
ORIGIN      
        1 ttagagcatt tttcgatccg ataaccgttt cacacttatc ggaaaatgct ctagagtttt
       61 acaggctggc gtaggccgcc tcgatcagcc gcaccgcgcg gggatcgccg atcagggcgt
      121 taccctgcct gttcatcaca taggatccgg acacgcctgt cgtcggatcg gcgaaggcgc
      181 aggagccgcc ccagccggaa tggccgaacg cctcggcggt ggggccgtaa tagaagttgg
      241 gcgtgttgcg catgaaaccc gcgccccagc tgatgtcgta gggcagcacc aggtcgcggc
      301 ccacgatccg ttcaaaggtg gcggccttga tcccggccgg actgaggatg gagcggccgt
      361 ccagcatgcc gccattggcc agggcgccca tcagtcgcgc cagggccggc gcggtggcgt
      421 gaccattggc cgagggaatc tcgacccgcc gccactcatc ggttcctcga cccgagggcg
      481 cggcccacgg ggtcatgaag gcggccttca cggcggcgtt catcgcgcca aacttgggca
      541 gggccgtggg gcgcatcagc tcggcggtgc ggccatgctc gctgtcgggc agaccgatcc
      601 acaggtccag gccaaacggc tgggcgatat cctcgcgcag ggcggttccc atcgtccggc
      661 catcgacgcg gcgaaagatc tcgccggcca gatagccgaa ggtcaccggg tgatagccgc
      721 tggccgagcc gaccggaaac agcggcgcca tggccgccag cttggcgcag gtggagtccc
      781 agtcgaacca gatggccggg tcggtctcgt cggggaaacc cgacagaccg gcctggtgcg
      841 acagggcctg ctcgacggtg acatcctgct tgccggcctg ggcgaactcc ggccagacgt
      901 cggcgacggg ctggtcatag gccaggcggc cctcgtcaac gaggcgggcg accatcaggg
      961 cggcgaccgc cttggtggtg gaaaacaggg ccgtcagggt gtcgggacca aaggcgatcg
     1021 tgcgcttgcg gtcagcatgg ccgcccatca ggtcgacgac gatctcaccc tcgacggcga
     1081 aggcgaacct cgcgcccagc tcgccgccgt cggcgaaatt ggcctcgaac acgtcgcgga
     1141 cccgagagaa gcggtcgggg cagacgccgg tgatgtcgac ggtcatggcg gcgatcccct
     1201 aggccagacg ctcgatgcgg accgtgggcg aggtctgctt gatccgcgcg cccatggcga
     1261 cgatcggcag ctcgggcgcg ttccagatgt tggcggcggt gacggtttcc gccacaccgc
     1321 cgaaggcgcg ggccaggccg taggacaggg tctggccttc caggatcgag gctgcgtaga
     1381 gggcggtcag caggtcgccg gtgcnnnnnn nnnngcgtgg gcggccagcc aggcctcctt
     1441 gctgtcggcc aggacgacgc cgatttccga gccccggtgg accgaagaca ccagcgtcgg
     1501 cttgcccagc agccgggcgg cgcgcatggc cgattgcgga tcacgggcgt cggcgccggt
     1561 cagcttttgc agctcccagg cgttgcaggc cacgacgtcg gcgcggggga tcagctcggc
     1621 ggcgatggcg tccgccacct cggccggcac atacaggccc ttgccggcgt cgcccatggt
     1681 gggatcgatg atgatcgagg ggcggcggct ggcggcccct tcgcggggcg cctcgcgcac
     1741 ggcgnnnnnn nnnnggcccg ggcggcggcg cgcacctggg cggcgctggc gaaatagccg
     1801 gtgatgacga tgtcggtcag gccaaacagg ccattggcct ccatgccgtc cagcatgcct
     1861 tccagcacct cgatcggtac gggcgccccg ccgggcacgc cccagccggg atggcggccg
     1921 aacaggacgg tcggggcgac catcgagtcg atgcggaact ggacaagcgc ggtggcctgg
     1981 gccgacgccc cgacccggct gccggcgaca tggctggaaa tgatcaacgc tagcggcatg
     2041 gcaagcggct tagcggacgc cggcgaactt gaacagggcg tcactcaggg gaagcggcga
     2101 cccggtcagg aaaggggcgg ggaagacgcc tcgctggcgt cggtcctgac gatggcccgg
     2161 gtgcgcgcca ggccgatctg ttcggcgatg atggagggcc aggcggcggg ctcttcccct
     2221 ctgaccgaca ggggcagctg catcacgcac tgcacgccct gcggcagata atcgatccgc
     2281 gcctcgccgc cgctttcctg gccaaaggtg cgcgcgatca gtcgcgtgcc aaatccggcg
     2341 cggtccggcg gcgtaacggg cggcccccaa gctcgcgcca caaaaggctg agctggcgcg
     2401 cgcccagctg agtgtgcgcc aggttccagg taatctccac ctgaccggcc tcggccgaca
     2461 gcgccccata tttgagggcg ttggtcgcca gttcatgcag ggccagggcc atggacaccg
     2521 cctggtgcgg actgagccag accagggggc catcgatctg gaagcggctg acgccctcat
     2581 gatcatgcgg ggctagcgcc tgccgcacga tggcgcccag atctgcgccc gcccagctct
     2641 cggcgaccag cacattgtgc gcgtccgata cggccatcag gcgattggtc aggtccttgc
     2701 ggatacgggg atcgaccttn nnnnnnnnng accttggcgg cccgcagggt ctgctcggtg
     2761 atcgattgca ccgtggccag ggtgttcttg atccgatggt tcagctcatt gagcagcagg
     2821 acccgctggt cttccgccag cttggcgggg gtctcgtcgc gcaggatctt catcagcccg
     2881 gtcagtttgg gctctaagat ccccatggtc atgccattgc cccagaagcg cgtaccgtcc
     2941 ttgcgaagat gccagcgggt gtcctcggcc ttgccatggt tcttggcggt ttccagttcc
     3001 agacgatcgg cgccggcctc gcgatcagag gcggtaaaca gctcggcgaa cggcaggccg
     3061 atggcttcca gccggtcata gcccaggatc ttctcggccc cccggctcca gctggtcacg
     3121 gcgccgtcca gatccatggt gaagatggcg tagccgaggg cgttttcgat aatcagatcg
     3181 gcgagtctgg cgtcgtggtc ggtggacatt gagggctcct gctccctaac aacgtcgcga
     3241 tgccgggttt cggcgcccgc cccacgaaaa aggcccgccg cgttt
//
LOCUS       DAOMEO010000242         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3293_length_3276_cov_3.096554, whole genome shotgun sequence.
ACCESSION   DAOMEO010000242 DAOMEO010000000
VERSION     DAOMEO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_3276_cov_3.096554"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLH31_06805"
     CDS             complement(<1..405)
                     /locus_tag="PLH31_06805"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQR88962.1"
                     /translation="MGTDAAKAPPSLDEVRWRLDAIDGELLKLLDERAALASSVAAAK
                     RAAGDTGFGLRPGREALIIRKLLAAPRTAANDALVIRIWREVMSDNLARQGPYHLSVW
                     GGRDTARAVELTRLRFGAAPPMRVMAEAKDVLT"
     gene            complement(499..1506)
                     /locus_tag="PLH31_06810"
     CDS             complement(499..1506)
                     /locus_tag="PLH31_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HQR88963.1"
                     /translation="MTHTDAAPPRKARRRGLLAPFIIALIFAAAWCAGWFWLRIQAEQ
                     RMDAASASLKSRGYDLSWSARSFSGFPFRLNVTLADARVAEPSGWALRIPELKAEAMI
                     YGLDHWVAVAPRGALLTRPNGGDVAIAGQALRASLAGLKEHPPRVSIEGAGLVFSTAP
                     EAAPFPLRSAQAMELHLRPGPDDQAAILFKANAARVNFTGLLGRIAQENSADMTLDAR
                     LTRASILRGDNWADAVRDWSAAGGAITMENARITAGEAEGTAKSGALTVDANGRLQGK
                     LEVALKERVDPKAARTPEQMLAAAAQALGREPVIEASLILQDGRTRLGTIDTGPSPRV
                     Y"
     gene            1535..2077
                     /locus_tag="PLH31_06815"
     CDS             1535..2077
                     /locus_tag="PLH31_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HQR88964.1"
                     /translation="MSSESGDRWVFGYGSLMWRPGFPFVERRRAVLHGRRRAFCIYSV
                     HHRGTYERPGLVLGLAPGGSVRGAAYRVAAGEWEAVYAYLQDREQPTETYFEAWSDLK
                     IDGEAGKVSSLVFLSDMKHSQWAGALSLEQQADLIAGAKGLSGRNIDYLSDLVGHLRE
                     DGVRDHAMETLLKLVEAREG"
     gene            complement(2074..2784)
                     /locus_tag="PLH31_06820"
     CDS             complement(2074..2784)
                     /locus_tag="PLH31_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR88965.1"
                     /translation="MIYLRSFLFHLYFWVWSVFTAIWMLALLPLPRRFAVAALTFWSK
                     GLFFGLRWLANIRIEVRGRENLPTGPALVAAKHQSMFDVFSQFGLLPDACFVMKKELL
                     NVPLFGWHGLKAGMIVVDRAGHSTALKKLVRDTLDRMKEVRQVVIFPEGTRGAVGAPG
                     DYKPGIAALYREMDLPVTPLALNCGSHWGKGFLCKPGTIVFHYLPPIPAGLKRGEFMR
                     ELQTRIDTATKALEDEGV"
     gene            complement(2781..>3276)
                     /locus_tag="PLH31_06825"
     CDS             complement(2781..>3276)
                     /locus_tag="PLH31_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQR88966.1"
                     /translation="LTGASNIRLEAATKLLEAGKGKRLLISGVNRDATREDVQTVTKA
                     VKPIYDCCVDLGFAAANTLGNAKETAEWAKAKGYRSLIVVTADYHMPRSMLELRATMP
                     DTELYPYPVATEGVDVHRWWKQNVTARRMIVEYSKYLAILGRETFLGLGPKDEAGTPA
                     EKTS"
BASE COUNT          526 a         1067 c         1158 g          525 t
ORIGIN      
        1 ggtcagcacg tccttggctt cggccatcac ccgcatgggc ggggcggcgc caaaacgcag
       61 gcgagtcagt tccacggcgc gagcggtgtc gcggccgccc cagacggaca ggtgataggg
      121 accctgacgc gccaggttgt cgctcatcac ctcgcgccag atgcggatga ccagggcgtc
      181 attggcggcg gtgcgcgggg cggccagcag cttgcggatg atcagggcct cgcggccggg
      241 gcgcaggcca aagcccgtat cgcccgccgc acgcttggcc gccgccaccg aactggccag
      301 cgctgcccgc tcgtccagca gcttcagcag ttcgccatca atggcgtcca gtcgccatcg
      361 gacctcgtcg agagacggag gcgccttggc cgcgtcggta cccatgttta tccccgaaat
      421 tggaggccgg accatagtcc ttgccgccgc cggcgcaata gctggcgttc acggcaagaa
      481 acttgcgcca gcggccgctt aatagacgcg cggcgaggga cccgtatcga tggttccaag
      541 gcgtgtgcgg ccgtcctgca ggatcaggct ggcctcaatg actggctcgc gtccaagagc
      601 ctgggcggcg gcggccagca tctgttccgg cgtccgggcg gccttgggat cgacgcgctc
      661 cttcagggcg acctccagct tgccttgcag gcggccattg gcgtcgaccg tcagcgcgcc
      721 ggacttggcc gtgccttcgg cttcacccgc cgtgatccgg gcgttttcca tggtgatcgc
      781 gccgccggcc gcgctccagt cgcgcaccgc gtcggcccag ttgtcgccgc gcaggatcga
      841 ggcgcgggtc aggcgggcgt ccagggtcat gtcggcgctg ttttcctggg cgatgcggcc
      901 cagcagtccg gtgaagttga cgcgggcggc gttggccttg aacaggatcg cggcctgatc
      961 gtcgggcccg gggcgcaggt gaagctccat ggcctgcgcc gagcgcagcg ggaagggggc
     1021 ggcttccggc gcggtcgaga acacaaggcc cgcgccctcg atggaaacgc gaggcggatg
     1081 ctctttcagg cccgccaggc tggcgcgaag cgcctggccc gcaatggcca cgtcgccgcc
     1141 attggggcgg gtcagaagcg cgccgcgcgg cgcgacggcc acccagtggt caaggccata
     1201 gatcatcgcc tcggccttca gctcgggaat gcgcagcgcc cagccggacg gttcggcgac
     1261 gcgggcgtcg gccagggtca cgttcaggcg gaacggaaag ccggaaaagc tgcgggcgct
     1321 ccacgacagg tcataaccgc gtgatttcag cgaggccgag gccgcgtcca tccgctgttc
     1381 ggcctgaatg cgcagccaga accagccggc gcaccaggcc gccgcgaaga tcagcgcgat
     1441 gatgaacggc gccagcaggc cccgtcggcg ggctttgcgg ggcggcgcgg cgtcggtatg
     1501 ggtcatgaaa gcgtgcgtcc agtcgggatt ggtgatgagc agcgagagcg gagaccgttg
     1561 ggtgttcgga tacggatcgc tcatgtggcg tcccgggttc ccgtttgtag agcgaagacg
     1621 cgcggttctc cacggccgtc gtcgcgcctt ctgcatctat tcggtccatc accgcggaac
     1681 ctacgagcgt cccggcctgg ttctgggcct cgcgcccggc ggctcggtgc gcggcgcggc
     1741 ctatcgggtg gcggcgggcg agtgggaggc ggtctatgcc tatctgcaag atcgggaaca
     1801 gccgaccgag acctatttcg aggcctggag cgatctgaag atcgacggtg aggcgggcaa
     1861 ggtctcctcg ctggtcttcc tgtcggacat gaagcacagc caatgggcgg gcgccctgag
     1921 cctggagcag caggccgacc tgatcgccgg cgccaagggc ctgtcgggcc gcaatatcga
     1981 ctatctcagc gaccttgtcg gacacctgcg cgaggacggc gtgcgggacc acgcgatgga
     2041 gacgctgctc aagctggtcg aggcgcggga gggctaaacc ccctcgtcct ccagcgcctt
     2101 ggtcgcggtg tcgatgcgag tctgcagctc gcgcatgaac tcgccgcgct tcaggccggc
     2161 ggggatgggc ggcagatagt ggaagacgat ggtgcccggc ttgcacagga agcccttgcc
     2221 ccagtgcgag ccgcagttca gggccagggg cgtcaccggc aggtccatct cgcgatagag
     2281 ggcggcgatg ccgggcttgt agtcgccggg cgcgcccacg gcgccgcggg tgccttcggg
     2341 gaagatcacg acctgtcgga cctccttcat ccggtccagc gtgtcgcgca ccagcttctt
     2401 cagggcggtc gagtgaccgg cccggtccac gacgatcatg cccgccttca ggccgtgcca
     2461 gccgaacagc ggcacgttca gcagttcctt tttcatcacg aaacaggcgt cgggcagcag
     2521 gccaaactgg ctgaagacgt cgaacatcga ctggtgcttg gcggcgacca gggccggacc
     2581 cgtcggcagg ttttcgcgtc cgcgcacctc gatccggata ttggccagcc agcgcaggcc
     2641 aaagaacaga cccttggacc agaaggtcag ggcggcgacc gcgaagcggc gcggcagggg
     2701 cagcagggcc agcatccaga tcgcggtgaa gaccgaccag acccagaaat agaggtggaa
     2761 cagaaacgaa cgcagataga tcaagacgtc ttctccgccg gtgtcccggc ctcatctttc
     2821 ggccccagac ccaggaacgt ctcgcgcccc aggatcgcca ggtacttgga gtattccacg
     2881 atcatccgtc gcgcggtgac gttctgcttc caccagcgat ggacgtccac gccctcggtg
     2941 gccacgggat aagggtagag ctcggtgtcg ggcattgtcg cgcgcagctc cagcatcgag
     3001 cgcggcatgt gatagtcggc cgtgaccacg atcaggctgc gatagccctt ggccttggcc
     3061 cactcggcgg tttccttggc gttgccaagg gtgttggcgg cggcgaagcc caggtcgacg
     3121 cagcagtcat agatcggctt gaccgccttg gtgacggtct ggacgtcctc gcgcgtagcg
     3181 tcgcgattga cgccggagat cagcaggcgc ttgcccttgc cggcctccag cagcttggtc
     3241 gccgcctcca gccggatgtt ggatgcgccg gtcagc
//
LOCUS       DAOMEO010000243         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3294_length_3276_cov_2.694194, whole genome shotgun sequence.
ACCESSION   DAOMEO010000243 DAOMEO010000000
VERSION     DAOMEO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_3276_cov_2.694194"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLH31_06830"
     CDS             complement(<1..815)
                     /locus_tag="PLH31_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR88967.1"
                     /translation="MITALVGGLLAFHFGPVPALTWAAINLALEAGLSYLHATFRPQA
                     KGRSSLVIRLAPAAAFSIVWSVMAALSAIHGTQSMKFAALIIRFGLIVEALKYATLSW
                     SAMLSIVPAPFIALFLAPLLGPNFSGWDRLLAVIVMLGLAGYIADAVRLMRSSAKALE
                     TAQAEAQAASRAKSAFVAMMSHELRTPMNGVLGLAHALGTTRLDDRQSDYLEMIIQSG
                     DSLMTILNDILDLSKVEAGKLALETAPFDIRRLGAQIHLVCNQTAQLKGLTLAL"
     gene            complement(991..2277)
                     /locus_tag="PLH31_06835"
     CDS             complement(991..2277)
                     /locus_tag="PLH31_06835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQR88968.1"
                     /translation="MANVTVVGAQWGDEGKGKIVDWLSNRADVVVRFQGGHNAGHTLV
                     VDGKVYKLALLPSGVVQGKLSVIGNGVVVDPWHLLSEIDKIADQGVIISPELLILADN
                     ACLILPLHKDLDQAREAASSQKIGTTGRGIGPAYEDKVGRRAIRVADLADPEALKPKI
                     ERLLSHHGALRRGLGLPEANADDLFDALMDLAPRILPYAQPAWRVLDQAYKAGRRILF
                     EGAQGALLDVDHGTYPFVTSSNTVAGQASAGSGMGPSATGYVLGIVKAYTTRVGEGPF
                     AAELDDEVGKHLSTVGREVGVNTGRARRCGWFDAVLVRQSVAINGIHGIALTKLDVLD
                     GLKTLKICVGYRIGDREVDYLPAGMRDQAAAVPIYEEMEGWTESTAGARSFKDLNANA
                     IKYVRRVEELIGAPVALLSTSPERDDTILMRDPFQG"
     gene            complement(2393..3001)
                     /locus_tag="PLH31_06840"
     CDS             complement(2393..3001)
                     /locus_tag="PLH31_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HQR88969.1"
                     /translation="MKTALFAAVAAATLGLTAPAMADEVFGGLYKHDVTFIGEAVGLG
                     AAGREDGVDVHLGYRTDRIESLTWLGKPQVHAFVSINSENTSNFAALGFNWKIELGQQ
                     GGFYLRPGFGLAYTDGEAGLPPANAPNLTPEERARRTNLYYTRIDFGSQVLFEPELAL
                     GYQFNDSWSAELSYTHLSNGQIFAQGKNQGLDDVGVRLVYAF"
     gene            3102..>3276
                     /locus_tag="PLH31_06845"
     CDS             3102..>3276
                     /locus_tag="PLH31_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQR88970.1"
                     /translation="MTITLSDIQAAAGRLKGHAVETPLIESPALNERLGGRVLLKPET
                     LQRAGAFKFRGAYN"
BASE COUNT          584 a         1059 c         1099 g          534 t
ORIGIN      
        1 agcgccaggg tcagaccctt cagctgggcg gtctggttgc agaccagatg gatctgggcg
       61 cccagccggc ggatgtcgaa gggcgcggtc tccagcgcca gcttgccggc ctccaccttc
      121 gacaggtcca gaatgtcatt gaggatggtc atcaggctgt cgcccgactg gatgatcatt
      181 tccagatagt cggactgacg gtcgtccagc cgtgtggtcc ccagcgcatg ggccaggccc
      241 agcacgccgt tcatcggcgt gcgcagttcg tggctcatca tcgccacgaa ggcggacttg
      301 gcgcggctgg cggcctgggc ctcggcctgg gcggtttcca gggccttggc cgagctgcgc
      361 atcagccgca cggcgtcggc gatgtagcca gccagaccca gcatgacgat caccgccaag
      421 agccgatccc agccactgaa attgggaccc agcaagggcg ccaggaacag ggcgataaag
      481 ggcgccggca caatggacag catggccgac caactgagcg tggcgtattt cagcgcctcg
      541 acgatcagtc cgaaccggat gatcagcgcg gcgaatttca tggattgcgt tccatggatc
      601 gcgctcagcg cagccatcac cgaccagacg atcgaaaagg ccgccgccgg cgccagacgg
      661 atcaccaggc tggatcggcc ctttgcctgc ggtctgaagg tggcgtgcag atagctcagc
      721 ccggcttcga gcgccaggtt gatggccgcc caggtcagcg ccgggacggg gccgaagtgg
      781 aaggccagaa ggcctcccac cagggcggtg atcatcaccc tcgccacgcg acttcggcga
      841 ctcatcacca gggtggcgcc ttccacccca tcattcagaa attcccgcag cgccaacctg
      901 cccaccccca caacgtcaag tcgagggaag cttgagggaa caaggttcaa agagcgttta
      961 ctttgcgcgc tagagtcgtc tttccgacgt ttaaccctgg aaggggtcgc gcatcagaat
     1021 agtgtcgtcg cgctcggggc tggtcgacag cagggccacg ggcgccccga tcagctcttc
     1081 gacgcggcgc acatacttga tcgcgttggc gttgaggtcc ttgaacgagc gggcgccggc
     1141 ggtgctttcg gtccagccct ccatctcttc gtagatcgga acggcggcgg cctggtcgcg
     1201 catgcccgct gggagatagt cgacctcgcg gtcgccgatc cggtagccca cgcagatctt
     1261 cagggtcttg agaccgtcca gcacgtccag cttggtcagg gcgatgccgt ggatgccatt
     1321 gatggccacc gactgacgca ccagaaccgc gtcgaaccag ccgcagcggc gggcgcggcc
     1381 ggtattgacg ccaacctcgc gacccacggt ggacaggtgc ttgccgactt catcgtcgag
     1441 ctcagcggcg aacggcccct cgccgacgcg ggtggtgtag gccttgacga tgcccagcac
     1501 atagccggtc gcagacgggc ccatgccgct gccggccgag gcctggccgg ccacggtgtt
     1561 ggacgaggtg acgaacggat aggtgccatg gtccacgtcc agcagggcgc cctgggcgcc
     1621 ctcgaacagg atgcggcggc cggccttgta ggcctggtcc agcacccgcc acgcgggctg
     1681 ggcatagggc aggatgcgcg gcgccaggtc catcagggcg tcgaacaggt cgtccgcatt
     1741 ggcttccggc aggcccaggc cgcgacgcag ggcgccgtgg tggctcagca agcgctcgat
     1801 cttgggcttc agggcttcgg gatcggcgag gtcggcgacg cggatggcgc ggcggccgac
     1861 cttgtcttca taggcaggcc caatgccgcg gccggtggtg ccgatcttct ggctggaggc
     1921 ggcctcgcgg gcctggtcca ggtccttgtg cagcggcagg atcaggcagg cattgtcggc
     1981 caggatcagc agctcgggcg agatgatcac gccctggtcg gcgatcttgt cgatctccga
     2041 cagcagatgc cagggatcga cgaccacgcc gttgccgatg acggagagct tgccctgcac
     2101 cacgcccgaa ggcagcaggg ccagcttgta gaccttgccg tccaccacaa gggtatggcc
     2161 ggcattgtgg ccgccctgga aacgcacgac gacgtccgcc cggttggaca gccagtcgac
     2221 gatcttgccc ttgccttcat cgccccactg ggcgccgacg acggtcacat tggccatgat
     2281 agagatatgc cttgtcacct gcccggtgac gcccagcggc ccttcgacag gactcggccg
     2341 cagacgtttt attcagttgt gggcggtggc ccgcctgaaa aggcgagcga agtcagaagg
     2401 cgtagaccag ccgaaccccc acgtcgtcaa gaccctggtt cttgccctgg gcgaagatct
     2461 ggccgttgga caggtgagtg taggacagct cggcagacca gctgtcgttg aactggtagc
     2521 ccagggccag ctcgggctcg aacagcacct gcgagccaaa atcgatgcgg gtgtaataga
     2581 ggttggtgcg gcgggcacgt tcctcgggcg tcagattggg ggcgttggcc ggcggcaggc
     2641 ccgcttcgcc gtcggtatag gccagaccaa agccgggacg cagatagaag ccgccctgct
     2701 gacccagctc gatcttccag ttgaagccta aggcggcgaa attggaggtg ttctcgctgt
     2761 tgatggacac aaaggcgtgg acctgcggct tgcccagcca ggtcaggctt tcgatccgat
     2821 cggtgcgata gcccagatgg acatccacgc catcctcgcg gccggcggcg ccaaggccca
     2881 cggcctcgcc gataaaggtc acgtcatgct tgtagaggcc gccaaagacc tcgtcggcca
     2941 tggcgggcgc ggtgaggccc agcgtggcgg cggccacggc ggcgaacagt gcggtcttca
     3001 tgtggtgatc cctgaacagc ccaacccgag ctggttatgg cgtgaacggc gagacccgca
     3061 aagggtttcg atcagaacgc ccgcgagcta gacctttgca catgaccatc accctctccg
     3121 acatccaggc cgccgccggc cgcctgaagg gccacgccgt cgagacgccg ctgatcgaga
     3181 gcccggccct gaacgaacgc ctgggcggcc gggtgctgct caagcccgag accctgcagc
     3241 gggccggggc tttcaagttt cgcggcgcct acaacc
//
LOCUS       DAOMEO010000244         3262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3313_length_3262_cov_2.337075, whole genome shotgun sequence.
ACCESSION   DAOMEO010000244 DAOMEO010000000
VERSION     DAOMEO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_3262_cov_2.337075"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2309
                     /locus_tag="PLH31_06850"
     CDS             <1..2309
                     /locus_tag="PLH31_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR88971.1"
                     /translation="SFTAQTQGTDMSSFSLSARLRGLSPNHTLLLVNGKRRHGSAILQ
                     VATGPFGGSAAPSLDLIPPDAVERIEILQEGAAAVYGTDAIAGVINLILKDDTEGGSF
                     KITGGQNYDSEGETFSVSGNVGFKLGEDGYFNLSGFHRRQDYTTTGTGQVQVTQLDGS
                     LQPNAPAQWSNLTGDALAGINGGQPKTYLTEFFYNMGYDFGGVELYSFGNYARRIGYA
                     KQGYRHPKRVCYESGNLGGSVTTAAYDPSICYGNTGVVGMVPLQHVTEDEYSFTVGAK
                     GEFGGGWNYDLSTTYGHDKNEIWTENSAHREVWQETYAQYLNGSGVLPNTPDSAYDGG
                     FRLSQTTVTADIRKEFDLGLADPLTFAFGGEYRRDTYTIVAGDYYSTYKTGVQSFPGY
                     KESDAGDWNRSAKAGYINVIVNPVEKWTVDLAGRYEDYSDFGDTLIGKATTRFDFSDA
                     VAIRGTVSTGFRAPTLAEQHYSTVAVGPTSAVAVLPAGSPAATLLGFSALKPEKSTNF
                     SGGFVFRPIPKLAVTLDGYRIALTAARYAVQGGVATAEAPAIISALQAAGVVFDAALP
                     NIGAQSFTNGIDTRTWGIDFSAAYPVALDFGSLNLSVSANYNKTKITKNKIPTVFSNI
                     SESYVEDASPDYKVVLGALFKSGAFTVNLRETFYGKTSVLVQPGVSSASLIAAGLPTI
                     YEAKVKPTGITDLEVSYAFNDAMTFSLGANNLFDKVPETPALLKGVTIPAGTSPYING
                     STTYNSPYGFGPYGTSGGYYYARIDFKF"
     gene            2467..>3262
                     /locus_tag="PLH31_06855"
     CDS             2467..>3262
                     /locus_tag="PLH31_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR88972.1"
                     /translation="MTDRPDTLSRRMLMRGAMGAAAIASAAPALAQRDRKVAMSDDPA
                     AMVATLTDGIFINSNENPLGPAPAALQALSGLDPLAGRYGMAFASKLESLFARQNGLA
                     PDQVQVHPGSFMPLRSVALTYSSKTRPIAYFEPTFDQGFLGKGNQPVTRAVALPLAGD
                     FTADVRKLLAAAPDAGVYYLCNPNNPTGLPIPRADIEWLLANKPAGSVLLVDEAYIHY
                     SDAQSCLDLVAKGADVLVTRTFSKIYGLAGLRCGLIAGRKDLLDGMA"
BASE COUNT          605 a         1139 c          949 g          569 t
ORIGIN      
        1 ccagcttcac cgcccagacc cagggcaccg acatgtcgag cttctcgctg tcggcccgtc
       61 tgcgcggcct gagcccgaac cacacgctgc tgctggtcaa tggcaagcgt cgccacggct
      121 cggccattct tcaggtcgcc accggcccgt tcggcggctc cgccgcgccc agcctcgacc
      181 tcattccgcc tgatgccgtc gaacgcatcg aaatcctgca ggaaggcgcc gccgccgttt
      241 acggcaccga cgcgatcgcc ggcgttatca acctgatcct gaaggacgat accgaaggcg
      301 gcagcttcaa gatcaccggc ggccagaatt acgacagcga aggcgaaacc ttctcggtct
      361 cgggcaatgt cggcttcaag ctgggcgagg atggctattt caacctcagc ggcttccacc
      421 gtcgccagga ctataccacc accggcaccg gccaggttca ggtgacccag ctcgacggtt
      481 cgctgcagcc caacgctccc gcgcaatggt cgaacctgac cggcgacgcg ctggcgggca
      541 tcaacggcgg ccagcccaag acctatctga ccgaattctt ttacaatatg ggctatgatt
      601 tcggcggcgt cgagctctac agcttcggta actatgcccg ccgcatcggc tatgccaagc
      661 agggttatcg ccaccccaag cgcgtctgct acgaaagcgg caatctgggc ggcagcgtca
      721 ccacggcggc ttatgatccc agcatctgct atggcaatac cggcgtcgtc ggcatggtcc
      781 cgctgcagca tgtgaccgag gacgaatata gcttcaccgt cggcgccaag ggcgaatttg
      841 gcggcggctg gaactatgac ctgtcgacca cctatggcca tgacaagaac gagatctgga
      901 ccgaaaattc ggcccatcgc gaagtctggc aggaaaccta tgcccagtat ctgaacggct
      961 cgggcgtcct gcccaacacg ccggactcgg cctatgacgg cggcttccgc ctcagccaga
     1021 ccacggtcac ggccgacatc cgcaaggaat tcgatcttgg cctggccgat ccgctgacct
     1081 tcgccttcgg tggcgaatat cgccgcgaca cctacaccat cgtcgcgggc gactattatt
     1141 ccacctacaa gaccggcgtt cagtccttcc cgggctacaa ggaaagcgat gcgggcgact
     1201 ggaaccgcag cgccaaggcc ggctacatca acgtcatcgt caacccggtc gaaaaatgga
     1261 ccgtcgacct tgccggccgt tacgaagatt attcggactt cggcgacacg ctgatcggca
     1321 aggccaccac ccgcttcgac ttcagcgatg cggtcgccat ccgcggcacc gtcagcaccg
     1381 gcttccgtgc gccgacgctg gccgaacagc attattcgac cgtggccgtc ggcccgacca
     1441 gcgccgttgc ggtgctgccg gccggttcgc ctgctgcgac cctgctgggc ttctccgcgc
     1501 tgaagccgga aaagtcgacc aacttctcgg gcggcttcgt cttccgtccg atccccaagc
     1561 tcgcggtcac gctggatggc tatcgcatcg ccctgaccgc ggcccgttat gccgtccagg
     1621 gcggcgttgc gacggcggaa gccccggcaa tcatctcggc gctgcaggca gctggcgtgg
     1681 tgtttgacgc ggcgctgccg aacatcggtg cgcagagctt caccaacggc atcgacaccc
     1741 gcacctgggg catcgacttc tcggccgcct atccggtggc tctggacttc ggcagcctga
     1801 acctgtcggt ttcggccaac tacaacaaga cgaagatcac caagaacaag attccgacgg
     1861 tcttcagcaa tatctctgaa agctatgtcg aagacgcttc gcccgactac aaggtcgtgc
     1921 tgggcgccct gttcaagagc ggtgccttca cggtcaacct gcgcgagacc ttctatggca
     1981 agaccagcgt gctggtgcag ccgggcgtgt cgagcgcttc gctgatcgcc gccggcctgc
     2041 cgaccatcta tgaagcgaag gtcaagccga ccggcatcac cgatctggaa gtcagctatg
     2101 cgttcaacga cgccatgacc ttctcgctcg gcgcgaacaa cctgttcgac aaggtcccgg
     2161 aaaccccggc cctgctgaag ggcgtgacga tcccggcggg cacctcgccc tatatcaacg
     2221 ggtcgaccac ctataacagc ccctatggct ttggccccta cggcacctcg ggcggctatt
     2281 attacgcgcg tatcgacttc aagttctgat aggctagaac tgaagggccg gtctcccagg
     2341 agaccggccc ttttgctttg ttcgcggtct gaagccggtc ggcatgaccg gcggctcgca
     2401 gaacgcggaa acgataatag ggatgtggat cgatgcctcg atccccatca gggggaagcg
     2461 aaaaccatga ccgaccgtcc ggacactctc agccgccgca tgctgatgcg cggtgccatg
     2521 ggcgcggcgg caatagccag cgcggcaccc gctctcgccc agcgcgaccg caaggtggcg
     2581 atgagcgatg atccggcggc gatggtcgcc acgctgaccg acggcatctt catcaacagc
     2641 aacgaaaacc cgctcggccc cgcgcccgcc gcgctgcagg cgctgtccgg gctcgatccg
     2701 ctcgccggcc gctatggcat ggccttcgcc tccaagctgg aaagcctgtt cgctcgccag
     2761 aacggcctgg cacccgacca ggttcaggtc catcccggct ccttcatgcc attgcgctcg
     2821 gtcgcgctga cctactcgtc gaaaacccgc ccgatcgcct atttcgagcc gaccttcgat
     2881 cagggcttcc tgggcaaggg caaccagccg gtcacccgcg ccgtcgccct gccgctggcc
     2941 ggcgacttca ccgccgatgt ccgcaagctg ctggccgccg cgcccgacgc tggcgtctat
     3001 tatctctgta atcccaacaa cccgaccggc ctgccgatcc cgcgcgccga catcgaatgg
     3061 ctgctcgcca acaagccggc cggctccgtg ctgctcgtcg acgaggccta tatccactat
     3121 agcgacgcgc aaagctgcct cgatctggtg gcgaagggcg cggacgtgct ggtcacccgc
     3181 accttctcga aaatctacgg cctggccggc ctgcgctgcg gcctgatcgc cgggcgcaag
     3241 gatctgctcg acggcatggc cg
//
LOCUS       DAOMEO010000245         3261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3314_length_3261_cov_2.629445, whole genome shotgun sequence.
ACCESSION   DAOMEO010000245 DAOMEO010000000
VERSION     DAOMEO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_3261_cov_2.629445"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1629
                     /locus_tag="PLH31_06860"
     CDS             91..1629
                     /locus_tag="PLH31_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQR88973.1"
                     /translation="MLSRRDSLLALMALPLLTPRDVFAAAGVNPFTLGVASGTPRADS
                     VILWTRLAVDPLRGGGMPRGRAEVRWTVWQDAEQRRVFREGRTWTSDADAHSVHVTLD
                     GLQPGREYWYQFSVGDAQSAVGRTRTSSAADRAAKLALASCNAWESGHFAAYADMADW
                     SPDCVIHVGDYIYEGGIGRLGVNTRKVGGRDVTSETVRLHNSPEIKSLWDYRNRYALY
                     KSDPSLQAAHAAAPWIVAMDDHEIDNNWAGDIPEDPDQQTLLEFKVRKLAALKAYYEH
                     MPIDRPPAINGLEASLPMYGAFRFGPALVHLLDTRQFRSDQPCGQGFPGDASCDAMND
                     PALTMTGGEQERWLLDSLKRSDAAFNVLASQTWFAPYRYNAPPEAPKVNMDQWDGYGV
                     QRQRIIDALADGVSNPVVLSGDWHAAAAMLIHQRPFDARSARVGHNFCGTSVSSNCPW
                     AGALDDAKSFNPHVDYVDGTKRGYIRSTVGSGDWVATYRTVDSPADPASRCSTAIEMR
                     TRDL"
     gene            1728..>2882
                     /locus_tag="PLH31_06865"
     CDS             1728..>2882
                     /locus_tag="PLH31_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88974.1"
                     /translation="MRRILCSALISLVLAAPVAAQDSYRAQTRWWKGNTHTHSWWSDG
                     DTPPEVVADWYKKRDYNFLVFSDHNAMQTGEKWYPIKRDATRQALAGYRAMFGDDWVA
                     SRTTDGVEEIKLKTLDDFRTLFEEPSSFIFVKGEEITDKFNNQPLHLNGVNLVEKIEP
                     RGGASVREVLQNDLNAVAEQSHRHGQAMLIHLNHPNFHYAVQAEDLIELEHKAGEGFF
                     EIYNGHPRVNNRGDALHIATERMWDVVLAHRLGRLGKTVVYGMAVDDAHEYTAWGLGE
                     TNPGRGWVMVRADRLTPNTIAEAMKRGDFYSSTGVTLKSLDIGEGSLSLEVEPQPGAE
                     YVIEFVGTLAKTDLASRGVVTPHAHEGKTEHTHQTVNTYNPDIGAVLKRVS"
     assembly_gap    2883..2892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          598 a         1062 c         1024 g          567 t
ORIGIN      
        1 tacggttgcg accaagttct aggcctcgcc cggtccgagg cggcgctccc gcctcggacc
       61 ttttggttct ttgaatggat gggaactctc atgctctcac gtcgcgattc gcttctggcc
      121 ctgatggccc tgccgctctt gacgccgcgc gacgtgttcg cagccgccgg cgtcaacccg
      181 ttcacactcg gtgtcgcctc gggtacgccg cgcgccgaca gcgtgatcct ctggacgcgc
      241 ctggcggtcg atccccttcg cgggggcggc atgccgcggg gccgggcgga ggttcgctgg
      301 acggtctggc aggacgccga gcagcgtcgg gtgtttcgcg agggccgcac ctggacctcg
      361 gacgctgacg ctcacagtgt tcacgtcacg ctcgacgggc tgcagccagg ccgggagtac
      421 tggtaccagt tcagcgtcgg cgacgcgcaa agcgcggtgg gccgcacgcg aacctcaagc
      481 gccgcagatc gcgcggccaa gctcgctctg gccagctgca atgcctggga gtcgggtcac
      541 ttcgccgcct atgccgacat ggcagattgg tctcccgact gcgtgatcca tgtgggcgac
      601 tacatctacg agggcggcat tggccgcctg ggcgtcaata cgcgcaaggt gggcggccgg
      661 gacgtcacgt ccgaaaccgt gcgcctgcac aacagccccg aaatcaagtc gctctgggac
      721 taccgcaacc gctacgccct ctacaaaagc gatcccagcc tgcaggccgc acacgcagcg
      781 gcgccctgga tcgtcgccat ggacgatcac gagatcgaca acaactgggc cggggacata
      841 ccggaagacc cggatcagca gaccttgttg gagttcaagg ttcgcaagct ggcggccctg
      901 aaagcctatt atgagcacat gccgatagac cgacctccgg ctatcaacgg gctggaggcc
      961 agcttgccaa tgtatggcgc cttccgcttt ggcccggcgc tcgttcatct gctcgacaca
     1021 cggcagttcc gttccgacca gccgtgtggc cagggcttcc ccggggatgc gtcctgcgac
     1081 gcgatgaacg atcccgccct gaccatgacc ggcggcgagc aggagcgttg gctgctggac
     1141 agcctcaagc gctcggacgc cgccttcaac gttctggctt cgcagacctg gtttgcgccc
     1201 taccgctaca atgcgcctcc ggaggcgccg aaggtcaata tggaccagtg ggacggttat
     1261 ggcgttcagc gccagcgcat catcgacgcc ctggccgatg gggtttccaa ccccgtggtg
     1321 ctcagcggcg attggcatgc ggccgccgcc atgctaatcc atcaacgacc ctttgacgcc
     1381 cgatccgctc gggttgggca caatttctgc ggcacgtcgg tatcgtcgaa ctgtccgtgg
     1441 gcgggagctc tggatgacgc caagtccttt aatcctcacg ttgactacgt ggatgggacg
     1501 aagcgcggct atatccgctc aacggtggga tcaggtgatt gggtcgccac ttaccggacg
     1561 gtggattctc cggccgaccc agcctcgcgt tgcagcaccg cgatcgaaat gcgcacccgc
     1621 gatttgtaat gcggccgtct gcggcgggca ctccgccgta ttcgtcccga caagcctccc
     1681 tctcaccgcg gcgcgcgttt tcgcgcctcc ctgcaggacc cctcgccatg cgccgcatcc
     1741 tctgttccgc cctgatctcc ctcgtcctcg ccgcgccggt ggccgcgcag gacagctacc
     1801 gcgcgcagac ccgctggtgg aaaggcaata cccacaccca cagctggtgg agcgacgggg
     1861 acacgccgcc ggaggtcgtc gccgactggt acaagaagcg ggactacaac tttctcgtct
     1921 tcagcgacca caacgccatg cagaccgggg agaagtggta ccccatcaag cgtgacgcca
     1981 cgcgtcaggc gctggccggc tatcgcgcca tgttcggcga cgattgggtc gccagccgaa
     2041 ccaccgacgg cgtcgaggag atcaagctca aaaccctgga cgacttccgc accctgtttg
     2101 aagaaccgtc gagcttcatc ttcgtcaaag gcgaggaaat cacggacaag ttcaacaatc
     2161 agccgctgca cctgaacggc gtgaacctgg tcgagaagat cgagccgagg ggcggggcga
     2221 gcgtccgcga agtgctgcag aacgacctga acgccgtggc cgaacagtcc caccggcacg
     2281 gccaggccat gctgatccac ctgaaccacc cgaactttca ctacgcggtc caggccgagg
     2341 atctgatcga gctcgagcac aaggccggtg aaggcttctt cgagatctat aatggccatc
     2401 cgagggtcaa caatcgcggt gacgcgctgc acatcgccac ggagcgcatg tgggacgtgg
     2461 tgctggctca ccgcctgggg cggctgggca agactgtcgt ctatggcatg gctgtcgatg
     2521 acgcccatga gtacaccgcc tggggcctgg gcgagaccaa ccctggccgg ggctgggtta
     2581 tggtgcgggc cgaccgtctg acgcccaaca ccatcgccga ggccatgaag cggggagact
     2641 tctactccag cacgggcgta accttgaagt cgctcgatat tggagaagga tcgctttcgc
     2701 tagaagtcga gccccagccc ggcgccgagt acgtcatcga attcgtcggt acgctggcca
     2761 agaccgatct ggcgtcccgc ggtgtggtta cgccccatgc gcatgaggga aagaccgagc
     2821 acactcacca gaccgtcaac acctacaacc cagatatcgg cgccgtgttg aagcgggtca
     2881 gtnnnnnnnn nnaggcggag taccgcgtga ccggcaagga actctatgtc cgcgcccggg
     2941 tgatctccac cttgccccat cccaatggtt cggtagcggg tgaaatgcag actgcctgga
     3001 cccagcccct cgtcgtcaag cgctgaagac ggatccacat ccgagctgac ctcggcgtca
     3061 gtatatggag gctttgggaa ggtgcggtga ggtggaatta aagtactgaa aaataacagc
     3121 cttccatgct tttgacgtcg gctgcgggac tattgcgggg ccttcacgca ttgcgggaaa
     3181 gccgctcgtc gccggcccgg gggagggggc agcctaatcc gccgagcggg gagacctcgc
     3241 gccttggcga caatcattcg g
//
LOCUS       DAOMEO010000246         3259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3317_length_3259_cov_2.537453, whole genome shotgun sequence.
ACCESSION   DAOMEO010000246 DAOMEO010000000
VERSION     DAOMEO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_3259_cov_2.537453"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLH31_06870"
     CDS             complement(<1..223)
                     /locus_tag="PLH31_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88975.1"
                     /translation="MAQPRPARSRALLGDRLAPAVSFVVAVQAVAAVFFIGDALADIR
                     NAGLSSHIAIEAVIALALLAGVAVGALQVR"
     gene            complement(223..870)
                     /locus_tag="PLH31_06875"
     CDS             complement(223..870)
                     /locus_tag="PLH31_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQR88976.1"
                     /translation="MSGAVHAPRRWDPLVRITHWGIAAAVVINRLVTEGGSLAHVWIG
                     LALAALLALRLGWGFVGPVEARFTSFPPSPRRAVEHVRDILAGRHVSHRSHNPLGALM
                     VYALWASLAVVIASGIAMTGFPPKVHAEGGERPAAASTVLVDEDEDAGEGEGEGEGEA
                     GGESGEIAEEVHEIAANLLLVLAALHLVGVAFETRRGGRQIVLAMIGGKGKIKEP"
     gene            complement(867..1139)
                     /locus_tag="PLH31_06880"
     CDS             complement(867..1139)
                     /locus_tag="PLH31_06880"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQR88977.1"
                     /translation="MRGLFIVIPFVFLGQTALAQRRLGEVIGPDKARAKASLAAQGYR
                     LTEFEAEHGRLEIKAVKGPSRIEIHAEEKTGKVLRVEVRKGRGERE"
     gene            complement(1268..2026)
                     /locus_tag="PLH31_06885"
     CDS             complement(1268..2026)
                     /locus_tag="PLH31_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQR88978.1"
                     /translation="MRRSVFAALSALVLSPLLAGCAYNADLGRQQMLIVNDSGLAAQG
                     EKAWAETLRTGLVSSDTRRNARIRTVGQRVVRAAGLDNRPWDYAVLIDEAPNAFVLPG
                     GHIGVTVGLLDLVDNDDQLAAVIGHEAGHVVAQHAAERYSQSVTTKLLLGVAGAAAGT
                     STDLGRNLGSYGGNATKYLFLLPFSRKHELEADRLGVDYMQRAGYRPQESVTLWRKMA
                     ALGGASGQPEIASTHPSDASRIAALQAYISSKGW"
     gene            complement(2112..2471)
                     /locus_tag="PLH31_06890"
     CDS             complement(2112..2471)
                     /locus_tag="PLH31_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR88979.1"
                     /translation="MRPDALRVLVVEDELFVAMLVEDLLGDLGCAIVGPAMTAAEAVL
                     AARTEHIDFALLDVNLDGETSFDAAEILRARGVPFAFVTGYGEQGVRVDLRDAPILSK
                     PIDPRHLAKVLGDFERH"
     gene            complement(2461..>3259)
                     /locus_tag="PLH31_06895"
     CDS             complement(2461..>3259)
                     /locus_tag="PLH31_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQR88980.1"
                     /translation="RYRETGERRIIGIGREVFGRRKDGTTFPLELSIAEWRDGGARYF
                     TGVMRDVTERKRAEELQRLLVNELNHRVKNTLATVQSVAGQTLRNATDLVDARESLTT
                     RLLALARAHDILTHESWDGADLRDVVASLINSHGDALRFRAEGPSVRLPPKVALSLSL
                     ALHELMTNATKYGALSTEPGRVVLSWNQTTEADVERLNLRWEEIDGPTVSTPSRQGFG
                     SRLITGGLARELGGDVRLDYPAGGVVCTINASLPRPGQPGRFIRGAA"
BASE COUNT          575 a         1134 c         1053 g          497 t
ORIGIN      
        1 gccgcacctg cagcgcacct accgccaccc cggccagcag ggccagggcg atgaccgcct
       61 cgatcgcgat atgcgagctg agtccggcat tgcggatatc ggccagggcg tcgccaatga
      121 aaaatacggc cgccaccgcc tgcacggcga cgacgaagga gaccgccggg gccaggcggt
      181 ctcccaagag tgcgcgggat cgggcggggc ggggttgggc catcacggct ccttgatctt
      241 gccctttccg cctatcatcg ccagcacgat ctgacgaccg ccgcgccgcg tctcgaacgc
      301 gacgcccacc agatgcaggg ccgccagcac cagcagaaga ttggccgcga tctcgtggac
      361 ctcctcggcg atctcgccgc tttcgccgcc cgcctcaccc tcaccctcgc cctcgccttc
      421 gccggcatcc tcgtcttcat ccaccaggac cgtcgatgca gccgccggcc gttcgccgcc
      481 ctcggcgtga accttgggcg gaaagccggt catggcgatc ccgctggcga tgaccaccgc
      541 caggctcgcc cacagcgcat agaccatcag ggcgcccagc ggattgtgtg accggtgcga
      601 gacatggcgg ccggcgagga tgtcgcgcac atgctcgacc gcgcgacgcg gactgggcgg
      661 gaaagatgtg aaccgcgcct cgaccggacc cacgaaaccc cacccaagcc gcagggccag
      721 cagggcggcc agggccaggc cgatccagac gtgggccagc gagccgcctt cggtcaccag
      781 acggttgatg accaccgcgg cggcgatccc ccagtgcgtg atgcgcacca gggggtccca
      841 gcggcgcggc gcgtgaaccg cgccgctcat tcgcgctctc cccgcccctt gcggacctcg
      901 accctgagca ccttgccggt cttttcctcg gcatggatct caatccgcga cgggcccttg
      961 acggccttga tctccagccg gccgtgctcg gcctcgaatt cggtgagccg atagccctga
     1021 gcggccaggc tggccttggc gcgggccttg tctggcccga tcacctcgcc gaggcgacgc
     1081 tgggccaggg cggtttggcc cagaaacaca aaaggaatga cgatgaacag accgcgcatg
     1141 ggaacctccg tcttgctgcg acggggttca tgaacggcgc gccgggagcc ggcgccatgg
     1201 acccagcgct tacggctcgg ccaataagcg tctgcttacg ctcatccttc cggagggcgt
     1261 caggccgtca ccagcccttg ctggaaatgt aggcctgtag cgcggcgatc cgcgaggcgt
     1321 ccgagggatg ggtcgaggcg atttcaggct ggcccgacgc cccgcccagg gcagccatct
     1381 tgcgccacag ggtcaccgac tcctgcggcc gatagccggc gcgctgcatg tagtcgacgc
     1441 ccaggcggtc ggcctccagc tcatgcttgc gcgagaaggg cagcaggaac aggtacttgg
     1501 tggcattgcc gccataggag cccaggtttc ggccaaggtc ggtcgaggtt ccggccgccg
     1561 cgcccgccac gccgagtagc agcttggtgg tcaccgactg cgagtaacgt tcggcagcgt
     1621 gctgggccac cacatggccg gcctcgtgac cgatcacggc ggccagctga tcatcattgt
     1681 cgaccaggtc gagcaggccc accgtcaccc cgatatggcc gcccggtaac acgaaggcgt
     1741 tgggcgcctc gtcgatcagc acggcatagt cccaaggccg attgtcgaga ccggcggcgc
     1801 ggaccacccg ctggcccaca gtgcggatcc gcgcattgcg gcgcgtgtcg ctggacacca
     1861 ggcccgtgcg aagggtttcg gcccaggcct tctcgccctg agcggccaga ccggagtcgt
     1921 tgacgatcag catctgctgg cggcccagat cggcgttata ggcacagccc gccaggagcg
     1981 ggctcagaac cagagcggaa agggcggcga aaacggaacg gcgcaagtcg gaactccggc
     2041 gggcaaggca ttctggttac acaaaaccgc acccgccggc ttgtccaatc gcatcgccgc
     2101 ggtgcgcacg ctcaatggcg ctcgaaatca cccagcacct tggccagatg gcgcggatcg
     2161 atgggcttgg agaggatcgg cgcatcacgc agatcgaccc gcacgccctg ctcgccatag
     2221 ccggtcacga aagcgaaggg tacgccccgc gcccgaagga tctcggccgc atcaaacgag
     2281 gtttcgccgt ccagattgac gtccagcaag gcgaaatcga tgtgttcagt gcgggccgcc
     2341 aaaacggcct ctgccgcggt catggccgga ccgacgatcg cgcagcccag atcgcccagc
     2401 aggtcttcca ccagcatggc gacaaacagc tcatcctcga cgaccagaac cctcagtgcg
     2461 tcaggccgca ccccggatga accgtccagg ctggccgggc ctgggaagac tggcgttgat
     2521 cgtgcagacg acgccgcccg ccgggtagtc gagacggacg tcgccgccca actcccgcgc
     2581 gagccccccg gtgatgaggc gactgccaaa gccttggcga cttggcgtag agaccgtggg
     2641 gccatcgatt tcttcccagc gtaaattcaa gcgttcaaca tcggcttcgg tggtctggtt
     2701 ccagctcagg accacgcggc cgggttcggt tgacagggct ccatacttgg tcgcgttggt
     2761 catcaactca tgcagggcca gggacaggga tagcgccacc ttcggcggca gcctcacgga
     2821 cgggccttcc gcccggaagc gcagggcgtc gccatgcgaa ttgatcagcg acgccaccac
     2881 gtcacgcagg tcagcgccgt cccagctttc atgtgtgaga atgtcatgag ccctggccag
     2941 ggccagtagc cgggtggtca gggactctcg cgcgtcgacc agatcggtgg cgttgcgcaa
     3001 ggtctggccg gccactgact ggacggtggc caaagtgttc ttgacccggt gattgagctc
     3061 attgaccagc aatcgctgca gttcctccgc gcgcttgcgc tcggtgacat cgcgcataac
     3121 gccggtgaaa tagcgcgcgc ccccatcacg ccactcggcg atcgacagtt ccagcgggaa
     3181 ggtcgtgcca tccttgcgcc ggccaaagac ctcgcggccg ataccgatga tccgtcgctc
     3241 gccagtttcc cgatagcga
//
LOCUS       DAOMEO010000247         3257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3321_length_3257_cov_2.592130, whole genome shotgun sequence.
ACCESSION   DAOMEO010000247 DAOMEO010000000
VERSION     DAOMEO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3321_length_3257_cov_2.592130"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1315
                     /locus_tag="PLH31_06900"
     CDS             137..1315
                     /locus_tag="PLH31_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQR88981.1"
                     /translation="MAAIFSRKPAYAASSPAPQAGGDLRARLLAAVSNPYIAASGAAC
                     LFLATLAGLVLVTGDPKAGAPVIRVELTKIGATRNAPDGWKEALADESEQEAAFKPDT
                     FDLSAKPIVDNPVEASISFPGEDSPAPIQAAGGALPQAPIAGLSGPGPGGAQLPIIAA
                     NGRTAAEAYARPFTASGRPKVSVVIGGLGLNPQVTRAAIETLPPEITLSFAPYAEGLQ
                     GWIDLARANGHEVLLETPMEPADYPANDPGPYTLIAANRPEDTVRKLEWLMSRATGYF
                     GLSNYLGARFVESDAAMNTFNAALKARGLAFVDDGLAARRGGSIPRASADRVIDDELS
                     AAAIDAQLRALETGASARGQSMGSGFAYPVTINQVRVWANGLSARGLQLAPASALARR
                     "
     gene            1312..1797
                     /locus_tag="PLH31_06905"
     CDS             1312..1797
                     /locus_tag="PLH31_06905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HQR88982.1"
                     /translation="MSQDPLPRHRPNVGVVLFHRDGRVWLGRRNQQPPPYNWQFPQGG
                     VDEGEDLLDAARRELAEETGVTSASLLGRTDGWISYDFPPEVAGSKMARGWMGQKQVW
                     FAFRFTGDESEIDLEAHGEVEFDGWRWGRLDETPELIVPFKRGVYEQVVAAFGKFVAG
                     D"
     gene            1824..2537
                     /locus_tag="PLH31_06910"
     CDS             1824..2537
                     /locus_tag="PLH31_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR88983.1"
                     /translation="MAKTVLMIHGYGCASDCWGPVAERLKAEGYKVETPTIRADQRKV
                     SGPGAGLAGLSLADYVADMSALAASLAQQDGEAPIVFGHSMGGLIAQKLAEAGLVSGA
                     VLFSPASPADARGRPKLAPVLAFLNAALMSNPQTRTVKQWKTGFKWGVMNAVPASRHD
                     ALYARMVHDSGRVLADLAWPDKDPNKTAHVDANRIKVPLRVPAPWTVRLPSRTCVWLA
                     RNIRLSAATMLNIRATPTG"
     gene            2462..>2739
                     /locus_tag="PLH31_06915"
     CDS             2462..>2739
                     /locus_tag="PLH31_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88984.1"
                     /translation="MRLVGQKYSAVGGDYVEYPSNAHWLIDEPGTDVILDDVIGWLKD
                     KQLAAGQTKAAASPPAEKAPARTSAAPKPKAAVKAKAAAKPVEAAPVKT"
     assembly_gap    2740..2749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2750..2997
                     /locus_tag="PLH31_06920"
     CDS             <2750..2997
                     /locus_tag="PLH31_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR88985.1"
                     /translation="PSKVATPAAKAKEKPKAAAPKATAPKAAAPAEAVAPAVKAKAAP
                     RARSAATPAAKPAKAPAAKPAATTKPAKSSPKPRKSV"
     gene            3043..>3257
                     /locus_tag="PLH31_06925"
     CDS             3043..>3257
                     /locus_tag="PLH31_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR88986.1"
                     /translation="MPAPVIMVHGAFCGGWTFDSFRQPFEAAGHVVLAPDLLGHDGKA
                     SVAGRSMTDYAKQIAALCATCEEPPIL"
BASE COUNT          551 a         1159 c         1047 g          490 t
ORIGIN      
        1 ggccattcgc cggctgaaaa acgagatact gcagcccgcc tgaccgacgg ggcgcgacag
       61 cgcgccgcgt aggcgcgctg tcagggtcca ggtttgttaa ccataaattt gaagcttgcc
      121 ggctagggtc gcgccaatgg ccgccatctt ttcccgcaag cccgcctacg ccgcgtcctc
      181 gcccgcccca caggccggcg gcgacctgcg cgcacgcctg ctggcggcgg tctcaaaccc
      241 ctatatcgca gccagcggcg cggcctgcct gttcctggcc accctggccg gcctggtgct
      301 ggtcaccggc gatcccaagg ccggcgcgcc ggtcattcgc gtcgagctga ccaagatcgg
      361 ggccacgcgc aacgctcccg acggctggaa ggaagctctg gccgacgaga gcgagcagga
      421 agccgccttc aagcccgaca cctttgatct ctcggccaag cccatcgtcg acaatccagt
      481 cgaggcgtcc atctcgttcc ctggcgagga cagcccggct cccatccagg ccgctggcgg
      541 cgccttgccc caggccccca tcgccggtct ttcaggtcct ggtccggggg gagcacagtt
      601 gccgatcatc gccgccaatg gccgcacggc cgccgaggcc tatgcccgcc cgttcaccgc
      661 cagcggccgg cccaaggtct cggtcgtcat cggcggcctg gggctgaacc cgcaggtcac
      721 ccgcgccgcc atcgagaccc tgccgcccga gatcaccctc tcctttgcgc cctatgccga
      781 gggcctgcag ggctggatcg acctggcccg cgccaacggc catgaggtgc tactggaaac
      841 accgatggag ccggccgact atccggccaa tgacccgggc ccctacaccc tgatcgccgc
      901 caatcggccg gaagacaccg tgcgcaagct ggaatggctg atgtcgcgcg ccacaggcta
      961 tttcggtctc agcaactatc tgggcgcgcg gtttgtggaa tccgacgcgg ccatgaacac
     1021 cttcaacgcc gccctcaagg ccaggggcct ggccttcgtc gatgacggcc tcgccgcccg
     1081 gcgcggcggt tcgattccgc gcgcctctgc cgaccgggtg atcgacgacg aactgtcagc
     1141 cgccgccatc gacgcccagc tgcgggccct ggaaaccggc gccagcgcgc gcggccagtc
     1201 gatgggttcg ggctttgcct atccggtgac catcaaccag gtgcgggtct gggccaacgg
     1261 cctgtcggcg cgcggcctgc agctggcgcc ggcctcggcc ctcgcccgtc gatgagccag
     1321 gatccgctgc cccgccaccg gcccaatgtc ggtgtggtgc tgttccatcg cgacgggcgc
     1381 gtctggctgg gtcgccgcaa ccagcagccc ccaccctata actggcagtt cccgcaaggc
     1441 ggcgtcgacg agggcgagga cctgctggac gccgccaggc gcgagctggc cgaggaaacc
     1501 ggcgtcacct ccgcaagcct ccttggtcgg accgatggct ggatttccta cgacttcccg
     1561 cccgaggtgg ccggctcgaa gatggcgcgg ggctggatgg gtcagaaaca ggtctggttt
     1621 gccttccgct tcaccggcga tgagagcgaa atcgacctgg aagcccatgg cgaagtcgag
     1681 ttcgacggct ggagatgggg aaggctcgac gaaacccccg aactgatcgt acccttcaag
     1741 cgcggggtct acgaacaggt ggtcgccgcg ttcggaaagt tcgtggcggg cgactgatcg
     1801 cccccacagg gagaacgatc ggcatggcca agaccgtcct gatgattcat ggatacggct
     1861 gcgcgagcga ctgctggggc cccgtcgccg aacgcctgaa ggccgagggc tacaaggtcg
     1921 aaacccctac tatccgcgcc gaccagcgca aggtttccgg gcccggagcg ggtctggccg
     1981 gcctcagcct ggctgactat gtcgccgata tgagcgccct ggccgcgtcg ctcgcccagc
     2041 aggacggcga ggcgccgatc gtgttcggcc attccatggg cggtctgatc gctcaaaagt
     2101 tggccgaggc ggggctggtc tcgggcgcgg tcctgttctc gcccgcctct ccggccgacg
     2161 cccgcggcag gcccaagctg gcgccggtcc tggccttcct gaacgctgct ctgatgtcga
     2221 acccgcagac caggacggtc aagcagtgga agaccggctt caagtggggc gtgatgaacg
     2281 ccgtccccgc cagccgtcat gacgccctct acgccaggat ggtccacgac tcgggtcgtg
     2341 tgctggctga cctagcctgg cccgacaagg atcccaacaa gaccgcccat gtcgatgcca
     2401 acaggatcaa ggtccctctg cgcgtgccgg cgccctggac cgtacgactc ccgtcgagga
     2461 catgcgtctg gttggccaga aatattcggc tgtcggcggc gactatgttg aatatccgag
     2521 caacgcccac tggctgatcg atgagccggg cacggatgta atcctcgacg acgtcatcgg
     2581 ctggctgaaa gacaagcagc tggccgccgg ccagaccaag gcggctgcgt cgcctccggc
     2641 cgaaaaggcg cccgccagga cgtcagctgc gccgaagccc aaggccgctg tgaaagccaa
     2701 ggctgcagcc aaacccgtcg aagcggcgcc cgtgaagacn nnnnnnnnnc cccgtccaag
     2761 gtcgctacgc cagccgcgaa agccaaggag aaacccaagg ccgccgcgcc gaaagctacc
     2821 gcgccaaaag ccgccgctcc ggccgaggct gtagctcccg cggtcaaggc caaggctgct
     2881 ccgcgcgcca ggagcgcggc gacaccggcg gccaagccgg ccaaggcgcc cgcggccaag
     2941 ccggctgcga caacaaagcc tgccaaatct tcgccgaagc ccaggaagag cgtgtaagct
     3001 caatccctcg gctgcttcgc cgacagggtt cgaaaggcgt tcatgcccgc accggtcatc
     3061 atggtccacg gcgccttttg cggcggctgg acgttcgaca gcttccgcca accgttcgag
     3121 gccgccggcc atgttgtgct ggcgccagat cttctgggtc atgacggcaa ggcctcggtc
     3181 gccggccgct cgatgaccga ctacgccaag cagatcgccg ccctgtgtgc gacctgcgag
     3241 gaacccccga tcctgat
//
LOCUS       DAOMEO010000248         3254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3326_length_3254_cov_2.288528, whole genome shotgun sequence.
ACCESSION   DAOMEO010000248 DAOMEO010000000
VERSION     DAOMEO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3326_length_3254_cov_2.288528"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLH31_06930"
     CDS             complement(<1..1000)
                     /locus_tag="PLH31_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="klcB"
                     /protein_id="HQR88987.1"
                     /translation="MAKRTKQPAQTGQDWSAPAAELLAELPADRDGLLAAAVAAVVEI
                     DAAVMRGDGAAAELAGDRYEAIIWKLNGGTNFGCMADDEAAGRVIERHCAAVPGDVPL
                     WGQRGQFLAVAGDVRALVEYEAGYGGPLNAHFQFHAVDLDRPFISATGYRSHFDTARG
                     CMTVDEVARGILTAMLAEKKRPVLIEANYRDRLADAPLPDWLAGLVPPARREPATVTI
                     PPGFVLVDVVLPAHKAFIARRWAAEAAGKVKAARAARSNAKGKAGGRERDPASAETAM
                     RCSTAKADDCKAEAGPVSPEATMPGAGEASCSTARNGDAGPADLVEFTPAPGQRCEI"
     gene            complement(1052..1480)
                     /locus_tag="PLH31_06935"
     CDS             complement(1052..1480)
                     /locus_tag="PLH31_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HQR88988.1"
                     /translation="MNTQDQPVTASLVAEAQRLDFLPTYFGPRLMMRGEALVYAWLRR
                     LCERYNGAYWHYYTLSDGGFYLAPDLAERLEIEVDGNGFRGELSADAAGIVATLFALG
                     QLAAEIAGTDAADALIDRYHFLRGFAAGHPEAAAIYRAID"
     gene            1649..1918
                     /locus_tag="PLH31_06940"
     CDS             1649..1918
                     /locus_tag="PLH31_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HQR88989.1"
                     /translation="MRTTQQLSITLPNDMADVVKTKVKTGEYASESEVIRDGLRALLA
                     RDRAVESWLHNQVGPAYDALKADPSRAVTADQVRARLAAEHAKAQ"
     gene            1915..2229
                     /locus_tag="PLH31_06945"
     CDS             1915..2229
                     /locus_tag="PLH31_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR88990.1"
                     /translation="MSYAVVFSPEAEEQLAELYRYIAAAASPDVAARYTEAIVTYCES
                     LRTFPLRGTMRDDVRPGLRITNYRKRAVIAFDVAGDLVSIIGVFYGGQDYETILHDDA
                     DD"
BASE COUNT          571 a         1108 c         1030 g          545 t
ORIGIN      
        1 cgatctcgca ccgttggcct ggtgcgggcg tgaactccac caggtcggcc gggcctgcat
       61 cgccattcct ggcggtgctg caggatgctt cgcctgcgcc cggcatcgtc gcttctggcg
      121 atacaggccc agcctcggcc ttgcaatcgt cagctttggc ggtgctgcag cgcatcgccg
      181 tttccgccga tgcgggatct cgctcgcggc ccccggcctt tcccttggcg ttcgacctgg
      241 cggccctggc cgccttgacc ttgcccgccg cctcggccgc ccagcgccgc gcgatgaacg
      301 ccttgtgcgc cggcagcacc acatcgacca agacgaagcc cggcgggatc gtcacggtcg
      361 ccggctcgcg ccgcgccggc ggcaccaggc cggccagcca atcgggcagg ggagcgtcgg
      421 ccaagcggtc gcggtagttg gcctcgatca acaccggccg cttcttctcg gccagcatgg
      481 ccgtcaggat gccgcgcgca acctcgtcca cggtcatgca gccccgcgcc gtgtcgaagt
      541 gcgagcgata gcccgtcgcc gagataaacg gcctgtccag gtcaaccgcg tggaactgaa
      601 aatgcgcgtt gagcggcccg ccataaccgg cctcgtactc caccagggcg cgcacgtcgc
      661 ccgccacggc caggaactgg ccgcgctggc cccacaaggg cacgtccccc ggcaccgcag
      721 cgcaatgccg ctcgatgacg cggccggcgg cctcgtcgtc ggccatgcac ccgaagttcg
      781 tgccgccgtt cagcttccag atgatcgcct cgtatcggtc gccggccagc tcggccgccg
      841 ccccgtcgcc gcgcatgaca gccgcgtcga tctccaccac ggccgcaacg gccgcagcca
      901 gcaggccgtc acggtctgcc ggcagctcgg ccagcagctc ggccgccggc gcgctccagt
      961 cctggcccgt ctgggccggt tgctttgttc gcttcgccat cgcttgacgt gctcctggtg
     1021 cagccccggc cggagccggg gccgcccctc atcagtcaat agcccggtag atcgcagcgg
     1081 cctcggggtg gccggccgcg aagccgcgca ggaagtgata gcggtcgatc agggcgtcgg
     1141 ccgcgtccgt gccggcgatc tcggccgcga gctggccgag cgcgaacagg gttgcgacaa
     1201 tgccggcggc gtcggccgac agttcgcccc ggaagccgtt gccgtctacc tcgatctcca
     1261 ggcgctcggc caggtcgggg gccaggtaga aaccgccgtc cgacagggtg tagtagtgcc
     1321 aatacgcgcc gttgtagcgt tcgcagagcc ggcgcagcca ggcgtacaca agggcctcgc
     1381 cgcgcatcat caggcgcggg ccgaagtagg taggcaagaa gtcgaggcgc tgggcctcgg
     1441 cgaccaggga agcggtgacg ggttggtctt gggtgttcat cggaactctc cggtagttga
     1501 cctgggcgac atgcccggcc ttcaagacgt atattaggac aaaatgtcct aacctgtcaa
     1561 gcactttagc taaactttag ccgctaaact ggacttcgcc cccttggagt gataggattg
     1621 aatcctattg aaccgaatgg aggccgccat gcgaaccacc cagcaactga gcatcaccct
     1681 gccgaacgac atggccgacg tggtgaaaac gaaggtcaag accggggagt acgcgagcga
     1741 aagcgaggtc atccgcgacg gcctgcgtgc gctactcgcg cgtgatcgcg ccgtcgagtc
     1801 ctggcttcac aaccaggtcg gcccggcgta tgacgccctg aaagccgatc cctcccgagc
     1861 cgtcaccgcc gaccaggtgc gcgcccgcct ggccgccgag catgcgaagg cgcaatgagc
     1921 tacgcggtcg tcttctcgcc cgaggccgaa gagcagcttg ccgagctgta ccgctacatc
     1981 gcggcggcgg cgtcgcctga cgttgccgct cgctacaccg aggcaatcgt cacctactgc
     2041 gaaagcctgc gcacgtttcc actgcgcggc accatgcgcg acgatgtgcg gcccggcctg
     2101 cgcatcacga actaccgcaa gcgcgccgtg atcgccttcg atgtggccgg cgatctggtt
     2161 tccatcatcg gcgtgttcta cggcgggcag gactacgaga cgatcctgca cgacgacgcg
     2221 gacgactgaa ttcccccgct tcttgacact tgaggggcgc aatcacgccc tgggcctggc
     2281 aggccgaccc ctcccttggc gttcaacctt gcggccttcg agctggagcc atcttccgac
     2341 ttggcgacgg cgcaggagtc accgcggcct tgacagttgg ggagtccaga gggggccttg
     2401 gccccctttg gaagagcacg aacttctgcc aggcgcttgc gcctggctat ggtcagccgt
     2461 agcgccgtag gcgctccatt cttggggtga aggccgtagg ccgaggggca aagcccctgg
     2521 ggggatggga ggccgcttta gcggccgggt gggatcgaga agggggggca cccccttcgg
     2581 cggccgatca ttctgcgggg caccttgggc cggtttgccg cttttttcga cacccggtta
     2641 taacaactag gtttaatata aaggttatga agggttaaaa gccggttagc ttgaccggaa
     2701 aacgcgcgca agccgttgat acgccaagga aaaggctcga agggccgccc ctcaagtgtc
     2761 aatggataag cccctcaagg gtcaatagtc tgcccgtcaa gtgtcaagga tcgcgcccct
     2821 catctgtcag cacccccgcc cctcaagtgt cagtacggca agggccttgc caacaggctt
     2881 atccacaact tctgtggata aagccagccc gatcaagcac ataggccgcc tcgacggcct
     2941 ccggcgcggc tccagccggc cgatttcgag gccggcccct catctgtcag catcgcgccg
     3001 gggtgcgctg ccccccaagt gtcaagaaac gcccctcaac tgtcaccagc cggcgttttg
     3061 tccccaaacg atccacaagg gggaaccgca gaattcggaa aaaatcgtac gctaagctaa
     3121 cggtgttctc gtgacagctc tttgactagg ctttctaagg ggtcgtctca gaaaacggaa
     3181 aataaagcac gctaaggcat agccgaacct gccaagcttg ctccaccctg tagtgacgcg
     3241 atcagcgggc agga
//
LOCUS       DAOMEO010000249         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3327_length_3253_cov_2.893996, whole genome shotgun sequence.
ACCESSION   DAOMEO010000249 DAOMEO010000000
VERSION     DAOMEO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_3253_cov_2.893996"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>212)
                     /locus_tag="PLH31_06950"
     CDS             complement(<1..>212)
                     /locus_tag="PLH31_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR88991.1"
                     /translation="GVFSHAVTKVAASGDFRVQPQFGGLVSSVAPEPEALRAAQMVLE
                     AADQPLTYARIDLIRGLDHTPQLMEL"
     assembly_gap    213..222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<223..760)
                     /locus_tag="PLH31_06955"
     CDS             complement(<223..760)
                     /locus_tag="PLH31_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR88992.1"
                     /translation="MTSPTADILILAPSSENAAYAAAGVRLFERLSAPLRARGLTVAS
                     QPWTDPVDPEAARLVLPLLAWGYHLRQPAWFAQLDALEAAGVPVINPVPTLRWNTTKT
                     YLIELESKGAPVVPTHAHDRLTPEALAAAFDAFGVDELVVKPQISGGSQDTVRVRRHD
                     DLQGGPPGAALIQPFLPAV"
     gene            885..1874
                     /locus_tag="PLH31_06960"
     CDS             885..1874
                     /locus_tag="PLH31_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HQR88993.1"
                     /translation="MTALYDVAAIGNAIVDVIAQCDDAFLAREGLVKGSMALIDPARA
                     ASLYDVMAAAIEASGGSAANTVAGVASFGGKGAFIGKVADDQLGNVFRHDMKAIGCAF
                     TTPSLAEGPATAQSLINVTPDAQRTMSTYLGACVELTSADVDPAIIEAARYSYLEGYL
                     FDPPEARRAFAKAAALAHGAGRQIAITLSDSFVVERHRAGLLGFIETQCDIVFANAAE
                     VCALFETDDFDAAVKALSEKVTIAAVTRSERGSVVAAGGQVHEISAYPVEKVVDTTGA
                     GDQYAAGFLFGLSQGRPLPVCGQLGSLAAAEVIDHYGPRPQVSLRELAQKNGL"
     gene            1882..2472
                     /gene="hisB"
                     /locus_tag="PLH31_06965"
     CDS             1882..2472
                     /gene="hisB"
                     /locus_tag="PLH31_06965"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQR88994.1"
                     /translation="MARTAEVVRETKETQIRVWIDLDGTGVSTISTGIGFYDHMLESF
                     ARHGGFDLKVETKGDLHIDMHHTVEDTGIVLGLAVNKALDGFKGIRRFGAAYIPMDET
                     LTRCAIDLSNRPYLIWKVDFKRPKVGEMDTELFKEFHHAFAMNCGACVHLETLYGDNT
                     HHVAESGFKALARALRQAVEIDPKTGGLAPSTKGVL"
     gene            2485..3126
                     /gene="hisH"
                     /locus_tag="PLH31_06970"
     CDS             2485..3126
                     /gene="hisH"
                     /locus_tag="PLH31_06970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQR88995.1"
                     /translation="MQTVALIDYGSGNLRSAEKALREAARRRGIAADIVVTAEPSLVA
                     AADRVVLPGVGAFASCRAGLDASGVYEAMNQAVHGRGVPFLGICVGHQLLATRGLEFG
                     VTPGLDWIPGEVRKLAPSDPSLTIPHMGWNAIQLTRDHPLFQGVENGAHMYYANSFAL
                     TPTDPADVLATTDHGGPFTAAVVKDNVAGVQFHPEKSQAAGLSLLANFLEWRP"
     gene            3114..>3253
                     /locus_tag="PLH31_06975"
     CDS             3114..>3253
                     /locus_tag="PLH31_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HQR88996.1"
                     /translation="MAPMILYPAIDLKDGQCVRLLHGDMEKATVFNNSPADQAERFVK
                     DG"
BASE COUNT          553 a         1110 c         1049 g          531 t
ORIGIN      
        1 ccagctccat cagttgcggt gtgtggtcca ggccccggat caggtcgata cgggcatagg
       61 tcagaggctg gtcggcggct tccagcacca tctgggccgc gcgaagggcc tctggctcgg
      121 gcgcgacgct gctgaccagg ccgccgaact gcggctgcac gcggaaatcg ccgctagccg
      181 cgaccttggt gaccgcatgg ctgaagacgc cgnnnnnnnn nnccacggcg ggcaggaacg
      241 gctggatcag ggccgcgccg ggcgggccac cctgcagatc atcgtgacgc ctgacccgca
      301 cggtgtcctg cgagccgccg gagatctgcg gcttgaccac cagctcatcg acgccaaagg
      361 cgtcaaaggc tgcggccagg gcttcgggtg tcaggcgatc atgggcatgg gtcggcacga
      421 cgggcgcgcc cttgctctcc aactcgatca gataggtctt ggtggtgttc cagcgcaggg
      481 tcggaaccgg attgatcacc gggacgccgg cggcctccag ggcgtccagc tgagcaaacc
      541 aggcaggctg gcgcaggtga tagccccagg ccagcaaggg caagaccagc cgggccgcct
      601 cgggatcgac cggatcggtc cagggctggc tcgccacggt caggccgcgg gcgcgcagcg
      661 gcgcggaaag tcgctcgaac aaccgtacgc ccgcagcggc ataggcggcg ttttccgaag
      721 acggcgcgag gatcaggata tcagcggtcg gtgaggtcat gcctggcggt tagaatgtcg
      781 cagggccagg ggcaagcctt cgttgccttg gcagcacgct tcctttactt gccagccctt
      841 aaacgctttg cgtcccctgc tctttcaaag gcttgccgat ctccatgacc gctctctacg
      901 acgtcgccgc catcggtaac gccatcgtcg acgtcatcgc ccagtgcgac gacgccttcc
      961 ttgcgcgcga gggtctggtg aagggctcga tggccctgat cgatccggcg cgggcggcca
     1021 gcctgtatga cgtgatggcc gccgccatcg aggcctcggg cggcagcgcc gccaacaccg
     1081 tggccggcgt cgccagcttc ggcggcaagg gcgccttcat cggcaaagtc gccgacgacc
     1141 agctgggcaa cgtatttcgc cacgacatga aggccatcgg ctgcgccttc accaccccgt
     1201 cgctggccga gggccccgcc acggcccaga gcctgatcaa cgtcacccct gacgcccagc
     1261 gcaccatgag cacctatctg ggcgcctgcg tggagctgac cagcgccgat gtcgatccgg
     1321 cgatcatcga ggcggcgcgc tattcctatc tggaaggcta tctgttcgat ccgcctgaag
     1381 cccgccgcgc cttcgccaag gccgccgccc tggcccatgg cgccggtcgt cagatcgcca
     1441 tcacgctgtc cgacagcttc gtggtcgagc gtcaccgcgc cggcctgctg ggcttcatcg
     1501 aaacccagtg cgacatcgtc ttcgccaacg ccgcagaggt gtgcgccctg ttcgagaccg
     1561 acgacttcga cgcggccgtg aaggccctgt cagaaaaggt caccatcgcc gccgtcaccc
     1621 gcagcgagcg gggctcggtg gtggccgccg gcggtcaggt tcacgaaatc tctgcctatc
     1681 ccgtggaaaa agtcgtggac acgaccggcg cgggcgacca atacgccgct ggtttcctct
     1741 ttggcctgtc ccagggccgc ccgctgccgg tctgtggcca gctgggctcc ctggccgccg
     1801 ccgaggtgat cgaccactat ggcccgcgtc cgcaggtttc cctgcgcgag ctggcccaga
     1861 aaaacggact gtaggacgag catggcccgc accgctgaag tggtccgcga gacgaaggag
     1921 acccagatcc gggtctggat cgatctcgac gggaccggcg tctcgacgat ctccaccggc
     1981 attggtttct acgaccatat gctggagagc ttcgcgcgcc atggcggctt cgacctgaag
     2041 gtcgagacca agggcgacct gcacatcgac atgcaccaca cggtggaaga caccggcatc
     2101 gtgctgggtc tggccgtgaa caaggcgctg gacggcttca agggcatccg ccgcttcggg
     2161 gcggcctata tcccgatgga cgagaccctg acgcgctgcg ccatcgacct gtccaaccgg
     2221 ccctatctga tctggaaggt cgatttcaaa cggcccaagg tcggcgagat ggacaccgaa
     2281 ctcttcaagg agttccacca cgcgttcgcc atgaactgcg gggcctgcgt ccatctggaa
     2341 accctgtacg gcgacaacac ccaccacgtc gccgagagcg ggttcaaggc cctggcccgg
     2401 gcgctgcgtc aggcggtcga gatcgacccc aagaccggcg gcctggctcc ctccaccaag
     2461 ggcgttctgt aggacactga tctcatgcag accgtcgccc tgatcgatta cgggtcgggc
     2521 aacctgcgtt cggccgaaaa ggccctgcgt gaggcggccc gtcgccgggg gatcgccgct
     2581 gacatcgttg tcacggcgga accctcgctt gtggccgccg ccgaccgcgt ggtgctgccc
     2641 ggcgtggggg cgttcgcctc gtgccgcgcg ggcctggacg cctcgggcgt ctatgaggcc
     2701 atgaaccagg ccgtgcatgg ccggggcgtt ccgttcctgg gcatctgtgt cggccatcaa
     2761 ctgctggcca cgcgcggtct ggaattcggg gtcacccccg gcctggactg gattcccgga
     2821 gaggtcagga aactggcgcc cagcgacccc agcctgacga ttccgcacat gggctggaac
     2881 gcgatccaac tgacgcgtga tcaccccctt tttcagggcg tcgaaaacgg cgcccacatg
     2941 tactacgcca actccttcgc cctgacgccg accgatccgg ctgacgttct ggcgaccaca
     3001 gaccacggcg gcccgttcac ggcggcggtg gtcaaggaca atgtcgcagg cgtacagttt
     3061 caccctgaga aatctcaagc cgcagggcta tccctgctcg ccaactttct ggaatggcgc
     3121 ccatgatcct ctatcccgcc atcgacctga aggacggcca gtgcgtgcgc ctgctgcacg
     3181 gcgacatgga aaaggccacg gtcttcaaca acagccccgc cgatcaggcc gaacgcttcg
     3241 tcaaggacgg ctt
//
LOCUS       DAOMEO010000250         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3328_length_3253_cov_2.848030, whole genome shotgun sequence.
ACCESSION   DAOMEO010000250 DAOMEO010000000
VERSION     DAOMEO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_3253_cov_2.848030"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PLH31_06980"
     CDS             <1..1337
                     /locus_tag="PLH31_06980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQR88997.1"
                     /translation="RLFQSIETHIRKGQLAEPILLADEFKKDPAFEELGGLRYLADLV
                     DRAPPAANVGDYARAVFDLALRRDLIRIGGDIATAAHGGGDEIRPARDQIEAAEQQLY
                     TLAESGTASSGFVTFGDALRGAVEMTAEAYSRDGGMSGVATDLVDLDQKIGGLHPSDL
                     VVLAGRPSMGKTALATNIAFNIARKYAYEIQPDGTKKTVQGGVVAFYSLEMSAEQLAL
                     RMLADASGVSGDRLRKGEIDASEFGRVRDAAMELQEAPLYIDATGGISIAKLTARARR
                     LKRQVGLDLVVVDYLQLVTGSDLGSNANRVQEVSQITMGLKALAKELGCPVIALSQLS
                     RQVEQREDKRPQLSDLRESGSIEQDADMVWFVYRESYYVGRAEPREGTPEHLTWQEDM
                     DRLQGLAEVIIAKQRHGPIGTVRLSFNSDTTRFGNLARDHYFAQARNIPSEE"
     gene            1409..2098
                     /locus_tag="PLH31_06985"
     CDS             1409..2098
                     /locus_tag="PLH31_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQR88998.1"
                     /translation="MDFSPLPVSTLVGAFLLAFPALFSIVNPVGASLIFDQAMGSRSR
                     DERLKLARSIGFYAFLVLLGSLLLGGYILNFFGVSLGALRVAGGLVVAIRAWGLLMDP
                     QEHEDRKTSDTEPARRQEDIAFFPMTMPFTTGPGSISVAIALSSQRPTEGSAVGFFIG
                     AGLAAAGVAVLVWLLYSASDRVTRLLGQSGARVLSRLVAFLLLCIGVQITASGFENLI
                     TVFLAAHPDAF"
     gene            2161..3243
                     /gene="alr"
                     /locus_tag="PLH31_06990"
     CDS             2161..3243
                     /gene="alr"
                     /locus_tag="PLH31_06990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQR88999.1"
                     /translation="MTDPSDARLTINLDALAANYQALKDAASGAEIAPAVKADGYGVG
                     AAIVADRLWQEGARSFYTARLSEGLALRQSLGDRDAVIHVLDGVTPGSAPAMEAARLT
                     PTLNSLPQIEAWNSHARGGRLNASLHIDTGMNRLGLRLEEVRVLLGAMDRLKHVEVTQ
                     VMSHLACANTPDHPLNVKQLERFHEATTLFPHARRSLANSAGIFLGGAYCFDQVRPGI
                     SLYGGGPRDVPDARIKAVVTLEAPILQTRNVPRGESIGYGATYTATDNTRVAIVAAGY
                     ADGVPRAAHPRGAVWFDGQRRPMLGRVSMDLIAVDVTDCDAARPGAMVQIIGPDLPVD
                     DAAAAAGTTAYELLTRIAPRALRVIG"
BASE COUNT          495 a         1182 c         1049 g          527 t
ORIGIN      
        1 agcggctgtt ccagtcgatc gagacccaca tccgcaaggg ccagctggcc gagccgatcc
       61 tgctggccga cgagtttaag aaggacccgg ccttcgagga actgggcggc ctgcgctatc
      121 tggccgacct cgtcgaccgc gccccgccgg cggccaatgt cggcgactat gcccgcgccg
      181 tcttcgacct ggccctgcgc cgcgacctga tccgcatcgg cggcgacatc gccacagcgg
      241 cccatggcgg cggcgatgaa atccgcccgg cccgcgacca gatcgaggcg gccgagcagc
      301 agctctacac cctggccgaa agcggcaccg cgtcttcagg cttcgtcacc tttggcgacg
      361 ccctgcgcgg cgcggtggag atgaccgccg aggcctatag ccgcgacggc ggcatgtccg
      421 gcgtcgccac cgacctggtc gatctcgacc agaagatcgg cggcctgcac ccctccgacc
      481 tggtggtcct ggccggccgt ccgtcgatgg gcaagaccgc cctggcgacg aacatcgcct
      541 tcaacatcgc ccgcaaatac gcttacgaaa tccagcccga cggcaccaag aagaccgtcc
      601 agggcggcgt cgtggccttc tattcgcttg aaatgagcgc cgaacagctg gccctgcgta
      661 tgctggccga cgcctcgggc gtctcgggcg accgcctccg caagggcgag atcgacgcca
      721 gcgagtttgg ccgggtgcgc gacgcggcca tggagctgca ggaagcgccg ctctatatcg
      781 acgccaccgg cggcatatcc atcgccaagc tgaccgcccg cgcgcggcgc ctgaaacgtc
      841 aggtgggcct ggatctggtg gtcgtcgact atctgcagct ggtcaccggg tcggaccttg
      901 gctccaacgc caaccgcgtt caggaagtca gccagatcac catgggtctt aaggccctgg
      961 ccaaggagct gggctgcccg gtgatcgccc tgtcgcagct gtcgcgtcag gtcgagcagc
     1021 gcgaagacaa gcgaccccag ctctccgacc ttcgcgaatc cggctcgatc gagcaggacg
     1081 ccgacatggt ctggttcgtc tatcgcgaaa gctactatgt cggtcgcgcc gagccccgcg
     1141 agggcacgcc cgagcacctg acctggcagg aagacatgga ccgcctgcag ggcctggccg
     1201 aggtgatcat cgccaagcag cgtcacggcc ccatcggcac cgtgcgcctg tcgttcaaca
     1261 gcgacaccac gcgctttggc aacctggccc gcgaccacta tttcgcccag gctcggaata
     1321 tcccgtcgga ggaatagacg gtggtttgac ctagcccgct gcatcgggcg agggtcgctc
     1381 gcccgccctc tggacagacg agtcgcccgt ggatttcagc cccctgcccg tctcgaccct
     1441 ggtcggcgcc ttccttctgg cctttccggc gctgttttcg atcgtcaatc cggtcggggc
     1501 ctcgctgatc ttcgaccagg ccatgggcag tcgtagccgt gacgagcgcc tgaagctggc
     1561 gcgcagcatt ggcttctatg cgttcctggt gctgctcggt tcactgctgc tgggcggcta
     1621 tatcctgaac ttcttcggcg tcagcctggg cgccctgcgg gtggccggcg gcctggtggt
     1681 ggcgatccgc gcctggggcc tgttgatgga cccgcaggag cacgaggacc gcaagaccag
     1741 cgacaccgag cctgccaggc ggcaggagga tatcgccttc ttcccgatga ccatgccgtt
     1801 cacaacgggg cccggctcga tctcggtggc gattgccctg tcctcacagc gcccgaccga
     1861 gggtagcgct gtgggcttct ttatcggcgc cggcctggcg gcggccgggg tggcggtgct
     1921 ggtctggctg ctctacagcg cctcggaccg ggtcacccgc ctgctgggac agtccggcgc
     1981 ccgggtactg tcgcggctgg tcgccttcct gctgctgtgc atcggcgtgc agatcaccgc
     2041 ctcgggcttc gagaacctga tcacggtgtt cctggccgcc cacccggacg cgttctagct
     2101 ggaaaagcgc cgccggctcc gctacgccct gacgcgccgc ttccttgttt cagagctctc
     2161 gtgaccgatc cttccgacgc ccgcctgacg atcaacctcg acgccctggc ggccaactat
     2221 caggctttga aggacgctgc gtcgggcgcc gaaatcgccc ccgccgtaaa agccgacggc
     2281 tacggcgtgg gcgcggcgat cgtcgccgac cgcctgtggc aggaaggcgc gcgcagcttc
     2341 tacacggccc gcctctccga gggtctggcc ctgcgccaga gccttggcga ccgcgacgcg
     2401 gtgatccacg tgctggacgg cgtgacgccc ggctccgccc cggcgatgga agccgcacgc
     2461 ctgaccccga ccctcaacag cctgccccag atcgaggcct ggaacagcca cgcccggggc
     2521 ggtcggctga acgcctccct gcacatcgac accggcatga accggcttgg cctgcgcctt
     2581 gaagaggtcc gcgtgctgct gggcgccatg gatcgcctca agcatgtcga ggtcacccag
     2641 gtgatgagcc acctggcctg cgccaacacg ccggaccatc cgctgaacgt caaacagctg
     2701 gaacgcttcc acgaggccac caccctgttc ccgcacgcgc ggcgcagcct ggccaattcc
     2761 gccgggattt tcctgggcgg cgcctattgc tttgatcagg tccggccggg gatcagcctc
     2821 tatggcggcg gcccgcgcga cgtgcccgac gcccgcatca aggccgtcgt cacgctggaa
     2881 gcgccgatcc tgcagacccg caacgtgccg cgcggcgaga gcatcggcta tggcgcgacc
     2941 tataccgcca ccgacaacac ccgcgtcgcc atcgtggccg ctggatacgc tgacggcgtg
     3001 ccgcgcgccg cccatccgcg cggcgcggtg tggttcgatg gccagcgccg tccgatgctg
     3061 ggccgggtct cgatggacct gatcgccgtt gatgtcaccg actgcgacgc cgcccgtccc
     3121 ggcgccatgg tgcagatcat cggcccggac ctgccggtcg acgacgccgc cgccgccgcc
     3181 ggcacgacgg cctacgaact gctgacccgg atcgcgccca gagccctgcg cgttatcggc
     3241 tgatcccacg tcc
//
LOCUS       DAOMEO010000251         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3332_length_3251_cov_2.298185, whole genome shotgun sequence.
ACCESSION   DAOMEO010000251 DAOMEO010000000
VERSION     DAOMEO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_3251_cov_2.298185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLH31_06995"
     CDS             complement(<1..1000)
                     /locus_tag="PLH31_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HQR89000.1"
                     /translation="MLGILAGVSALAIGGSLIWALQSSRPADPAELYNTDNRSTADGL
                     SRLPADYAGLPRQAPPLGPPLPGDLGRPMLNAGVHPEPLPGAPAPAAPAPDPEAQRLA
                     QERQRLAQEREAARASRLFANETQTSAEGGGPVAGVPSGSPDSVLRDVAAAAGGGPSA
                     PASDAERRLAFLNGNADRRTASAEALQAPISPFTVQAGAVIAAAMVTGLRSDLPGQIT
                     AQVTENVYDSPTGRILLIPQGARLIGQYDAEVAFGQSRALLVWNRLILPNGRSIVLER
                     QPGADASGYAGLEDEVDNHWGQLFRGAVLSTLLSIGAEAGTSSDESDLLQAIRRGAS"
     gene            complement(1114..>1790)
                     /gene="trbG"
                     /locus_tag="PLH31_07000"
     CDS             complement(1114..>1790)
                     /gene="trbG"
                     /locus_tag="PLH31_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HQR89001.1"
                     /translation="ADGALYQVYTAPGQVTDIALQPGEQLVGAGPVAAGDTVRWIIGD
                     TESGSGANRRLHILVKPTRADLQTNLVINTDRRTYHLELRAAERTYMASVSWSYPQDE
                     LIALRRQNAEARAAQPAATSPFDVLESLNFRYRIEGDNPAWRPVRAFDDGRQVFIEFP
                     NGIAQGEMPPLWIIGAQGGAELVNYRVRGRYMVVDRLFGGAELRLGADRQRRVRIVRI
                     DGRPAS"
     assembly_gap    1791..1800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1801..2126)
                     /locus_tag="PLH31_07005"
     CDS             complement(<1801..2126)
                     /locus_tag="PLH31_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HQR89002.1"
                     /translation="MRLTTLSLVLLASVSVAACSHTWTPPDITYDAAATPQPAVLEAE
                     PPKAVEIVEIPTPLPLPGQLQRIPEARPQPPEPADPRARIEAANSAARVQPSRSGYFN
                     AVQVYP"
     gene            complement(2126..2812)
                     /gene="trbF"
                     /locus_tag="PLH31_07010"
     CDS             complement(2126..2812)
                     /gene="trbF"
                     /locus_tag="PLH31_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HQR89003.1"
                     /translation="MMLFKRSSTRYGRTPEPETPYQRAAQAWDDRIGSARVQARNWRL
                     MAFGSLFLSAGLAAAVVWQAASGTVVPWVVQVDRLGQAQAVAPADVSYRPTDPQIAWH
                     LARFIEQTRAIPADPIVLRQNWLRAYDYTTDQGALALNDYARVNDPFAKLGREQVSIE
                     VSSVIRASPDSFRVAWVERRYVDGALAATERWTAILTITIQTPRDVERLRKNPLGVYV
                     HALNWSKELG"
     gene            complement(2809..>3251)
                     /locus_tag="PLH31_07015"
     CDS             complement(2809..>3251)
                     /locus_tag="PLH31_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HQR89004.1"
                     /translation="VLAGAAGARMAGGAALAAVRGGASVAGGASTAYQLGSAAKGGSV
                     AGGLSGVAQAGASAAASPLRKAAADLGQRYQAGGRAAFDGLGGRVSGASPANDAGSAP
                     GEPAWAKAMRRRQSAAHGVQTAVHAVRAGDGGGAGSSVSVREGE"
BASE COUNT          473 a         1104 c         1184 g          480 t
ORIGIN      
        1 cggacgcgcc tcgtcggatg gcctgcagga gatcgctctc gtccgacgat gtaccggctt
       61 cggcgccgat gctcaggagc gtggacagca cggcgccgcg gaagagctgg ccccaatgat
      121 tatcgacctc gtcctcaagt ccggcgtagc cggaggcgtc ggcgcccggt tgccgttcca
      181 gaacgatcga gcgcccgttg ggcaagatca gacggttcca gaccagcaag gcgcgcgatt
      241 gtccgaacgc gacctcggcg tcgtactggc cgatgagccg cgcgccctgc gggatgagga
      301 gaatccgccc ggtcggactg tcgtagacgt tctcggtcac ctgggcggtg atctgtccgg
      361 gaagatcgga gcgaagcccg gtcaccatcg cggcggcgat caccgcgccc gcctgaaccg
      421 taaaaggact gatcggcgcc tgcagcgctt cggcgctcgc tgtccggcgg tccgcgttcc
      481 cattgaggaa ggccagacgc cgctccgcat cgctcgccgg cgctgaggga ccgccacccg
      541 ccgcggcggc aacgtcacgc aggacgctgt ctggcgaacc cgacggtacc ccggcgacgg
      601 gcccgccgcc ctcggcggaa gtctgcgtct cgttggcgaa cagccgactg gcgcgggcgg
      661 cttcgcgctc ctgagcaagc cgctggcgtt cctgcgccaa tcgctgggct tcgggatcgg
      721 gcgcgggcgc tgctggcgcc ggcgcgccgg ggaggggctc ggggtgaacg cccgcattca
      781 gcattggtcg gccaagatct cccgggagcg gcggcccaag cggcggcgct tgccgcggca
      841 aacccgcata gtcggcaggc aggcgcgaca ggccgtcggc cgtggagcga ttgtcggtgt
      901 tgtagagctc ggccgggtct gcggggcgac tggactgaag cgcccagatc aggctgccgc
      961 caatcgccag cgcggatacg ccggcgagga tgccgagcac cttgcgcgac agtcgaacga
     1021 ccttgggccg ctctggtcgc aggcgcagct cctcggcgat ctcgttttcc gggcgcggcc
     1081 gatcggccgg ggtgccatcg ggatcgccca ccgtcacgac gccggcctcc cgtctatgcg
     1141 cacgatccgc acgcgccgct ggcgatcggc gccgagacga agctccgcgc cgccgaagag
     1201 tcggtcaaca accatgtagc gaccgcgaac gcggtagttg accagctcgg cgccgccttg
     1261 ggccccgatg atccacaacg ggggcatttc gccctgggcg atgccgttgg gaaactcgat
     1321 gaacacctgg cgcccgtcgt cgaaggcccg gaccggccgc caggccggat tgtcgccctc
     1381 gatgcggtag cggaaattga gcgactccag aacgtcgaac ggactggtcg ccgcaggctg
     1441 cgccgcccgc gcctctgcgt tctggcggcg cagggcaatg agttcgtcct gcggatagga
     1501 ccaggagacg ctcgccatat aggtccgctc ggccgccctc agctcgaggt gataggtccg
     1561 acggtcggtg ttgatgacga gattagtctg caggtccgcg cgggtcggct tgaccagaat
     1621 atggagtctc cggttcgcgc cgcttccgct ttcggtgtcg ccgatgatcc agcgcaccgt
     1681 gtcgccggcg gccacgggac ctgcgccgac caattgctcg cccggctgca aggcgatgtc
     1741 ggtgacctgg cccggcgcgg tatagacttg gtagagcgcg ccgtcggcat nnnnnnnnnn
     1801 taggggtaga cctgcaccgc attgaagtag ccggaccgac taggctggac gcgtgcggcg
     1861 ctgttggcgg cctcgatgcg tgctcgcggg tcggccggct cgggcggctg cggtcgcgcc
     1921 tccgggatcc gctgcagctg gccgggaagc ggcaggggcg tcgggatttc gacgatctcg
     1981 actgctttgg gcggctccgc ttccaggacc gccggctgcg gggtcgccgc agcgtcatag
     2041 gtgatgtcgg gaggtgtcca ggtgtgggag caggcggcga cggacaccga cgcgagcaaa
     2101 acgagagaga gtgtggtcag tcgcatcagc cgagctcctt ggaccagttg agggcgtgga
     2161 cgtagacgcc gagcgggttc ttacgcaggc gctccacgtc gcgcggcgtt tggatggtga
     2221 tggttagaat ggccgtccac cgctcggtgg cggccagggc gccgtcgaca tagcggcgtt
     2281 cgacccacgc tacgcggaag ctgtcgggcg aggcccggat gacgctggag acctcgattg
     2341 agacctgttc gcgcccgagc ttggcgaagg ggtcgttgac gcgggcgtag tcattgaggg
     2401 ccagcgcgcc ctggtcggtg gtgtagtcgt aggcgcgcag ccagttctgg cgcagcacga
     2461 tgggatcggc cgggatggcg cgcgtctgct caatgaagcg ggccagatgc caggcgatct
     2521 gcggatcggt cggccgatag ctgacgtcgg ccggtgcaac cgcctgcgcc tgcccgaggc
     2581 gatcgacctg caccacccac ggcaccacgg tgcccgaggc ggcctgccag accacggcgg
     2641 ccgcaaggcc ggccgagaga aagagcgacc caaacgccat gaggcgccag ttgcgggctt
     2701 gcacacgcgc cgagccgatc cggtcatccc aagcctgggc cgcgcgctga tacggcgttt
     2761 cgggttcggg cgttcggcca tagcgcgtcg acgatcgttt gaagagcatc actcaccctc
     2821 tcggacactg acggaagatc ctgcgccgcc gccgtcgccg gcccgcacgg cgtgcacggc
     2881 ggtttggacg ccatgcgccg ccgactgccg ccgccgcatg gccttggccc aggcgggctc
     2941 gcccggcgcc gaaccggcgt cattggcggg gctcgcgcca gagaccctgc cgcccaggcc
     3001 gtcgaaggcg gcgcgtccgc cggcctgata gcgttggcca aggtccgcgg ccgccttgcg
     3061 cagcggcgac gccgccgccg aggcgcccgc ctgtgcgacg cctgaaagac cgcccgcgac
     3121 gctgccgccc ttggcggcgc tgcccagctg gtaggcggtc gatgcgccgc cggcgacgct
     3181 cgccccgccg cgcacggcgg ccagcgccgc gccgcccgcc atgcgcgcgc cggccgcgcc
     3241 ggccagcact g
//
LOCUS       DAOMEO010000252         3238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3337_length_3238_cov_2.299403, whole genome shotgun sequence.
ACCESSION   DAOMEO010000252 DAOMEO010000000
VERSION     DAOMEO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_3238_cov_2.299403"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLH31_07020"
     CDS             <1..494
                     /locus_tag="PLH31_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HQR89005.1"
                     /translation="ALAAVACMAAASEPNERLADPAQEARARVLFKEVRCLVCQNESI
                     DDSQADLAADLRRIVRQEIAKGQSDREIRAFLVARYGEFVLLKPQFSAGNVVLWLAPL
                     GILAAGGGLMVALLRRRREDGATEPDEAAGLSAAEEARLAALLEDKGHDCAEKTPQEA
                     PSA"
     gene            630..2201
                     /locus_tag="PLH31_07025"
     CDS             630..2201
                     /locus_tag="PLH31_07025"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HQR89006.1"
                     /translation="MTARKSGFIVGAVAGAGVAAAALAGMGMRMGAADAAEAPAVVRT
                     STAAAPSFAPPPGAPMSFADIFEKVSPAVVQIDVTSKANPARGLRIPGLEGFDIVPRG
                     QRPGAEGEEDTPAAPKQQSSGSGFFISADGYIVTNNHVVADADEINVVMKDGRELKAT
                     VVGRDEGTDLAVIKVTDPKAKNAEFPYVNFENQAKPRVGDWVITIGNPFGLGGTATAG
                     IISAYNRDLGDSSSRFVDYIQIDAPINRGNSGGPSFDIYGRVIGVNTAIFSPTGGSVG
                     IGFAIPADVAESTAKQLIAGGKVVRGYIGANIQDFSEEMAEAQGMPDVKGAIIAELVV
                     GAPAAKAGLMPEDIVTAVNGVNVKSGSELTRQVAKARPGEAIRLSILRGGKPRTIEVK
                     SGVRPAEDALGANDNSNEDGGAPATPDKPQVQKFEALGLTLAPLDTAARATYKIAPEV
                     RGVLIAGVKADSDAGEKGLVKGDVLSSVNGVAVNSTGEVAAAISAAEKSGRTSVLAKV
                     VRQGRPVFVPLKIAP"
     gene            2392..3063
                     /locus_tag="PLH31_07030"
     CDS             2392..3063
                     /locus_tag="PLH31_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR89007.1"
                     /translation="MRILIIEDDIEAAAAMAHGLTEAGYECVHAADGELGLKEAGKGG
                     FDVLIVDRMMPKKDGVEVVETLRREGDNTPVLFLSALGEVGDRVVGLKAGADDYLVKP
                     YAFPELIARVEALSRRRETGAVATTLKVGELEMNLINRTVHRQGKEIDLQPREFQLLE
                     FMMRHAGQSVTRTMLLEKVWEYHFDPQTNVIDVHISRLRSKIDKGFDRAMLQTVRGAG
                     YRLDP"
BASE COUNT          552 a         1057 c         1100 g          529 t
ORIGIN      
        1 cggctctggc cgccgtcgcc tgcatggctg cggcgtccga gccgaacgag cgcctggccg
       61 atcccgcgca agaagctcgc gcccgtgttc tgttcaagga agtacgctgc ctcgtctgcc
      121 agaacgaatc catcgacgac tcccaggccg accttgccgc agacctgcgg cgaattgtcc
      181 ggcaggagat cgccaagggc cagagtgatc gcgagattcg cgcgttcctg gtcgcccgct
      241 acggcgaatt cgtgctgctg aagccgcagt tttcggcggg caatgtcgta ctgtggctgg
      301 ccccgctggg gattttggcg gccggcggcg gcctgatggt cgcgcttctg cgtcggcgac
      361 gcgaggatgg cgcgaccgaa ccggacgagg cggcgggcct gtctgcggcg gaggaggccc
      421 gactggcggc cctgctggaa gacaaaggtc acgattgcgc cgaaaagacg ccgcaagaag
      481 caccctccgc atgacggaaa gatagttcga gaaccattgc gccttacgcc atcgcgacta
      541 ggttctcttg caaggcataa tcttcggcgc acagcgccga gggcgaacag gtcccggcgc
      601 tgtgcgctgc gggtgaggaa agacaagata tgaccgctag gaagtcgggt ttcatcgtgg
      661 gcgccgttgc cggtgcgggc gtcgccgccg ccgccctggc gggcatgggc atgcggatgg
      721 gtgcggcgga cgccgccgag gcccccgccg tggtccgcac gtccacggcg gccgccccgt
      781 cgttcgcgcc gccgcccggc gcgccgatgt ccttcgccga catcttcgaa aaggtttccc
      841 cggccgtcgt ccagatcgac gtgacctcca aggccaatcc ggcgcgcggc ctgcgcatcc
      901 ccggtctgga aggcttcgat atcgtgccgc gcggccagcg gccgggcgcc gagggcgagg
      961 aggacactcc ggctgcgccc aagcagcagt cctcgggctc gggcttcttc atctcggccg
     1021 acggctacat tgtcaccaac aaccacgtcg tggccgacgc cgacgagatc aatgtcgtca
     1081 tgaaggacgg ccgcgagctg aaggccaccg tggtgggccg cgacgagggc accgacctgg
     1141 cggtgatcaa ggtcaccgat cccaaggcca agaacgccga gttcccttat gtgaacttcg
     1201 agaaccaggc caagccgcgg gtcggcgact gggtgatcac catcggcaac ccgtttggtc
     1261 tgggcggcac cgccacggcg ggcatcatct cggcgtataa ccgcgacctg ggcgacagct
     1321 cctcgcgctt tgtcgactac atccagatcg atgcgcccat caaccggggc aattcgggcg
     1381 gtccgtcgtt cgacatctat ggccgggtga tcggcgtgaa cacggcgatc ttctcgccga
     1441 cgggcggctc tgtcggcatc ggctttgcca ttcccgccga cgtggccgag agcaccgcca
     1501 agcagctgat cgccggcggc aaggtggtgc gcggctatat cggcgccaac atccaggact
     1561 tctccgaaga gatggccgaa gcccagggca tgcccgatgt gaagggcgcc atcatcgccg
     1621 aactggtggt gggtgcgccc gccgccaagg cgggcctgat gccggaagac atcgtcaccg
     1681 ccgtcaatgg cgtcaacgtc aagagcggct cggaactgac ccgccaggtg gccaaggccc
     1741 ggccgggcga ggccattcgc ctgtcgatcc tgcgcggcgg caagccccgc acgatcgagg
     1801 tcaagtcggg tgttcgaccg gccgaagacg ccctcggcgc caatgacaac agcaatgagg
     1861 acggtggcgc tccggccacc cccgacaagc cgcaggtcca gaagtttgaa gccctgggcc
     1921 tgaccctggc cccgctcgat acggcggcgc gcgcgaccta caagatcgcg cccgaagtgc
     1981 gcggcgtgct gatcgcgggc gtgaaggccg actccgacgc aggcgagaag ggcctggtga
     2041 agggcgacgt tctgtccagc gtcaatggcg tggcggtcaa ttcgaccggc gaggtggccg
     2101 ccgccatttc ggccgccgaa aagagcggcc gcaccagtgt gctggccaag gtggtccgtc
     2161 agggacgtcc ggtcttcgtt ccgctgaaga tcgcgccgta gtcactagcc cggctgcgca
     2221 aagcaagatc agtcccgctc cggttcttcc ggggcgggac gttttgtatc tgtccaaggt
     2281 ttaagggacg ggcggaccca aaaccggtat ccatcgccga ggatcgcgtc ctagggtcga
     2341 atcgggagaa cgtcagtttt ccgttcgtgg gcgcatgagg gatttgggct gatgcgtatt
     2401 ctgatcatcg aagacgatat cgaggcggcg gccgcgatgg cccacggcct taccgaggcg
     2461 ggctatgaat gcgtccacgc cgccgacggc gagctgggcc tcaaggaggc cggcaagggc
     2521 ggcttcgacg tgctgatcgt cgatcgcatg atgccgaaaa aggatggcgt cgaggttgtc
     2581 gagaccctgc gccgcgaggg cgacaacacc cccgtgctgt tcttgtcagc gctgggcgag
     2641 gtgggcgacc gggtcgtagg cctgaaggcc ggcgccgacg attatctggt caagccctat
     2701 gccttccccg agctgatcgc ccgcgtcgag gccctgtcgc gccgtcgcga gacgggcgcc
     2761 gtggccacca ccctgaaggt gggcgagctt gagatgaatc tgatcaaccg cacggtccat
     2821 cgccagggca aggagatcga tctccagccc cgcgagttcc agttgctgga attcatgatg
     2881 cgccacgccg gccagtcggt gacccgcacc atgctgctgg agaaggtgtg ggaataccac
     2941 ttcgacccgc agaccaacgt catcgacgtg catatctcgc gcctgcgcag caagatcgac
     3001 aagggctttg accgcgccat gctgcagacc gtgcgcggcg ccggctatcg gctcgatccc
     3061 tagaagatag ggcggttcgc cgccgaggcc tgtcatgcgt ctgccgcgtc ttctccgcac
     3121 cacgccgttt cggctgaccc tgttgttcct ggccctgttc gctgcggcgg ccagcgcctt
     3181 cctgggctat atctatgtgg ccacggccgg cgaggtgaac cgccgcgccg acgccgag
//
LOCUS       DAOMEO010000253         3234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3342_length_3234_cov_2.371500, whole genome shotgun sequence.
ACCESSION   DAOMEO010000253 DAOMEO010000000
VERSION     DAOMEO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_3234_cov_2.371500"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>703)
                     /locus_tag="PLH31_07035"
     CDS             complement(<1..>703)
                     /locus_tag="PLH31_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR89008.1"
                     /translation="AERRALLPFILVAISATLISSILLHRLIAEPVMRLARAADRVRL
                     EGARAISLPDIAKRQDEIGDLSRALEEMTHSLSERMDGIERFAADVAHEIKNPLTSIR
                     SAIETLDLVSDPVARARLLAILQNDVNRLDRLVTDISNASRLDAELSREAPRALDLAR
                     LLAEVASLYEAQLRPGDAPGSVRVTVQTGEPGVPARLMARETPIGQVFRNLIDNARSF
                     SPADGEVRVTLARARG"
     assembly_gap    704..713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(714..1347)
                     /locus_tag="PLH31_07040"
                     /pseudo
     CDS             complement(714..1347)
                     /locus_tag="PLH31_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515613.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
     gene            complement(1322..>1398)
                     /locus_tag="PLH31_07045"
     CDS             complement(1322..>1398)
                     /locus_tag="PLH31_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HQR89009.1"
                     /translation="FRQVDPEFDSIETLYGVGYRYREA"
     assembly_gap    1399..1408
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1409..2062)
                     /locus_tag="PLH31_07050"
     CDS             complement(<1409..2062)
                     /locus_tag="PLH31_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR89010.1"
                     /translation="MAAITLIDDDENIVASVSLALESHGHTVKAYYDGASGLEAVESH
                     APDLVILDVKMPRMDGMEVLRRLRQTSEIPVIMLTSKDDEIDEILGFNLGADDYMHKP
                     FSQRLLLERVKAVLRRARPEDEEPSIAAGLAGSKMMKRGKLTLDPARHDSLWDGKPVR
                     LTVTEFLLLQSLAQRPGFVKSRDNLMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDD
                     "
     gene            2300..>3234
                     /locus_tag="PLH31_07055"
     CDS             2300..>3234
                     /locus_tag="PLH31_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89011.1"
                     /translation="MSDVHYELFVRRKVGAQWVLEMATEVRTAALQNAEECLTSGRAV
                     AVRVSKETLDRESGEYKSIAIFTKGMVDGGKPKKDAEDLDPLCVQPADLYTAHARDRI
                     GRLLEGWLARHNATPFELLHRPDLVEKLEASGTDLQHALQKIAIPEAEARGMSVHELI
                     RTFQGLVERSVANLMKAFRKGALPDLDKEGFAKAATRLSTDPDRAFLLGAGVAASIAP
                     GISWSDKIARLLDLADAAPTEPGPRALALHAIEQPLAEIIGSRAGMADLLGTADDLGS
                     TLAAMTRLTGGAQVEALIRMQAQVRACMPELSGTA"
BASE COUNT          507 a         1099 c         1094 g          514 t
ORIGIN      
        1 ggccccgcgc cctggccagg gtgacgcgga cctcgccgtc ggcggggctg aaggagcggg
       61 cgttgtcgat caggttgcga aacacctggc cgatcggggt ctcccgcgcc atcagccggg
      121 cggggacgcc gggctcgccg gtctgaaccg tgacccgcac gctgcccggc gcgtcgccgg
      181 ggcgcagctg ggcctcatac aggctggcga cctcggccag cagcctggcc aggtccaggg
      241 cgcgcggcgc ctcgcgggac agctcggcgt ccaggcgcga ggcgttggag atgtcggtga
      301 ccagccggtc caggcgattg acgtcgttct gcaggatggc cagcagccgc gcccgggcca
      361 cggggtcgga caccaggtcc agggtctcga tggccgagcg gatcgaggtc agggggttct
      421 tgatctcgtg ggccacatcg gccgcgaacc gttcgatgcc gtccatccgc tcggacagtg
      481 aatgggtcat ctcctccagg gcgcgggaca ggtcgccgat ctcgtcctgg cgcttggcga
      541 tgtcgggcaa ggagatggcg cgcgcgcctt ccaggcgcac ccggtcggcg gcgcgggcca
      601 ggcgcatcac cggctcggcg atcaggcgat gcagcaggat cgaggaaatc agggtggcgc
      661 tgatcgccac caggataaag ggcagcaggg cccggcgctc ggcnnnnnnn nnnagtcgac
      721 gtcgccggct tccagggtca gcacgcccag cacggcgcgc acatgctgga tcgggatcga
      781 caccgaaacg acccggtcgc cgttctcgga catgcgggtg ccggccacca tcgtgccacg
      841 cagggcctgg gtgacttcat tgcgcagacc ctcgcgggcc ttttcagcct tggcctcgtc
      901 agccggatcc ttgcgcgtgg tctcgtcggt ctggccgggc ttgcgggccg ggggcagcac
      961 cttccactcg acgcggtcgg cgaccacgaa gctgtcagcc agcaccttgc cctgggcgtc
     1021 gaacagcctg gcccgctgcg agcgcgggat gaacagcagc tggaagatgg cgctggccgt
     1081 gtagggatcc aggcccggct cgggctcgcc caccgtcgcc gactggtcga tgacgttggc
     1141 gatcagctcg ccctgggtgg tcaggctgtc gatccgcgca ttgaccaggc cgctgcgcag
     1201 ctcgttgagc accagcgccc cgatcaccag gatcgccagc gccagcacgt tcaggccaat
     1261 gaccagccgg cccagacgcg agccacgcgg ccaggcccag cgacgccgcg gcgcgtcggc
     1321 gtcaggcttc gcgataacgg tagccaacgc cgtacagggt ctcgatggaa tcaaactccg
     1381 gatcgacctg gcggaactnn nnnnnnnngt cgtcgtcgac caccttgaac ttcttgcgca
     1441 gccgcttgat gtggctgtcg atcgtgcggt cgtcgacata gacctgatcg tcgtaggcgg
     1501 cgtccatcag gttgtcgcgg ctcttcacga agccgggacg ctgagccagg gactgcagca
     1561 gcaggaactc ggtgacggtc aggcgcacgg gcttgccgtc ccacaggctg tcgtggcggg
     1621 ccggatcgag ggtcagcttg ccgcgcttca tcatcttgga gccggccagg cccgcagcga
     1681 tcgagggctc ctcgtcttca ggacgggcgc ggcgcagcac ggccttcacc cgctccagca
     1741 gcaggcgctg gctgaagggc ttgtgcatgt agtcgtcggc gccgaggttg aagccgagga
     1801 tctcgtcgat ctcgtcgtcc ttggaggtca gcatgatgac cgggatctcg gaggtctggc
     1861 gcaggcggcg cagcacttcc atgccgtcca tgcgcggcat cttgacgtcg aggatgacga
     1921 gatccggcgc gtggctttcg acggcttcga ggcccgaagc gccgtcgtag taagccttga
     1981 ccgtgtggcc atggctttcg agggccaggg agaccgaagc gacgatgttt tcgtcgtcgt
     2041 caatgagcgt gatagcggcc atggagcccg tctgatccct tatgtcgtcc gtcgaacgat
     2101 cctatcgacc gcccgtctct gcttcaacgc gcggtccagc aaagctttcc cgatcgggtt
     2161 tgtccggcct ttgcggcccc agcgtggcgc gattgcggct ggacggctct ggcggcgcca
     2221 agcttcacca caatggcggt cctcaacctc gctttaaggt cttgtatccc ataaccgagg
     2281 ctcggggagc ccttagccca tgagcgatgt ccattacgaa cttttcgtgc gccgcaaggt
     2341 tggcgcgcag tgggtcctgg aaatggccac ggaagttcgc acggctgcgc tccagaacgc
     2401 cgaagagtgc ctgacatcag gccgcgcggt cgccgttcgc gtcagcaagg aaaccctgga
     2461 ccgcgagtcc ggcgaataca aatccatcgc catcttcacc aagggcatgg tcgacggcgg
     2521 caagcccaag aaggacgccg aggacctgga cccgctctgc gtccagccgg ccgacctcta
     2581 taccgcccac gcccgcgacc ggatcggccg cctgctggaa ggctggctgg cgcgacacaa
     2641 cgccacgccg ttcgaactgc tgcaccgtcc cgacctggtc gaaaagctgg aagcctccgg
     2701 caccgacctg cagcatgccc tgcagaagat cgccattccc gaggccgaag cccggggcat
     2761 gtcggtccac gaactgatcc gcaccttcca gggcctggtc gagcgctccg tcgccaacct
     2821 gatgaaggcc ttcaggaagg gcgccctgcc ggatctggac aaggaaggct tcgccaaggc
     2881 ggcgacccgt ctgtcgaccg atcccgaccg cgccttcctg ctgggggccg gtgtcgccgc
     2941 ctcgatcgcg cccggcatta gctggtcgga caagatcgcc cgcctgctgg acctggccga
     3001 cgccgccccg accgagcccg gtccccgcgc cctggccctg cacgccatcg aacagcccct
     3061 ggccgagatc atcggctcgc gcgccggcat ggccgacctt ttgggcaccg ccgacgacct
     3121 tggctcgacc ctggcggcca tgacccggct gaccggcggc gcccaggtgg aagccctgat
     3181 ccgcatgcag gcccaggtcc gcgcgtgcat gcccgagctg tcgggcacgg ccaa
//
LOCUS       DAOMEO010000254         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3346_length_3230_cov_2.478110, whole genome shotgun sequence.
ACCESSION   DAOMEO010000254 DAOMEO010000000
VERSION     DAOMEO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3346_length_3230_cov_2.478110"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLH31_07060"
     CDS             complement(<1..941)
                     /locus_tag="PLH31_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89012.1"
                     /translation="MRIGFLFNHDQVHQVAHSLPIALALAHGAQGVEIVVATTNRRLT
                     AEVIRLGGGLIGQGLTLVELGLTRRSSKLLAAATEAVLPASKLLVYSDNLDFFRSLDI
                     LVVAEKTSLILKSRYGLGDLKIVHTRHGAGDRAIGFDKASAGFDHVLVSGRKIRDRLI
                     GETGVDPGKISIVGYPKFDLLPAHKTTHPLLDDGRPVTLYNPHVSPHLSSWYEAGRQV
                     LDWFVEHDDHNLIFAPHVMLFERPFVATIDKLRLDRAGRIEERYLRAPNIHIDLGSRA
                     STTMAYTQRADLYLGDVSSQVYEFLLKPRPCVFLNNHR"
     gene            complement(1006..1278)
                     /locus_tag="PLH31_07065"
     CDS             complement(1006..1278)
                     /locus_tag="PLH31_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89013.1"
                     /translation="MIDALLTDDDIASLIASKSGLARSVLQRSTRICDLGLRLDAYMA
                     VLVAIEDRFDVEIDDLVGADCATLGALMDCLKARIVEQAAPPCRAA"
     gene            complement(1342..2016)
                     /locus_tag="PLH31_07070"
     CDS             complement(1342..2016)
                     /locus_tag="PLH31_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR89014.1"
                     /translation="MSPSLVAQAVCKRYGAGVQQVDALHPVSLSLRPGELVMITGPSG
                     SGKSTLLAIISGLLSPDAGVVEALGRPLQVMSADQRDRFRLAYSGFIFQGFHLLPALT
                     ASEQVEIVLRQLGVSAREARSRAIEALEAVGLGRRLASRPGEMSGGEKQRVAIARALA
                     KRPALIFADEPTSALDSENGRQIAGLLRAQAQASGATVVCVTHDGRLADLADRVLRME
                     DGRVRA"
     gene            complement(2013..>3230)
                     /locus_tag="PLH31_07075"
     CDS             complement(2013..>3230)
                     /locus_tag="PLH31_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR89015.1"
                     /translation="GSPWSPDLGDPVLADLLVRAELGSLDLKMALARLERAGADVDLA
                     RAGRRPHLTVGAELANGGADFSSVRSGAGTPLQASYEVDIFGRLKHGQDAAMADEAAS
                     LQDAAGVRRRVLAEVAHAYVALRAAQAHQAAAEVLKPLVGQVSDLIRRRQADGAATSA
                     EVVAARAQEDQAGGELQRATHAVEAERTRLGILLGEDAPIDEPAYAGDEIPLTPPITA
                     VPSEAVLGRADILAAQARLRAADARRAEAVAASRPRFMLTAGIGSGDTDLLYLLDVRA
                     LAWAVAGGLTHQLFDGGAAKARKRGATADAALAELAYRKAVGEAWGETRLALAALQDA
                     TTAEALARKRWRQDVLAFGTGQTLHRDGDIDGVALAALEVRAAAADTTLADARAARAQ
                     AYIDLSLALGGVS"
BASE COUNT          554 a         1096 c         1073 g          507 t
ORIGIN      
        1 cggtggttgt tgaggaagac gcatggccgg ggcttgagca ggaactcgta gacctgactg
       61 ctgacgtcac ccagatagag atcggcgcgc tgggtgtagg ccatggtcgt cgaggcccgg
      121 ctgcccagat cgatgtgaat gttgggcgcg cgcagatagc gttcctcgat ccgtccggcg
      181 cgatcgagcc ggagcttgtc gattgtcgcc acgaagggcc gctcgaacag catcacatgg
      241 ggcgcaaaga tcagattgtg gtcatcgtgc tcgacaaacc agtccagcac ctggcgacca
      301 gcctcgtacc aggacgacag atgcggcgag acgtgcggat tgtagagcgt caccggccgg
      361 ccgtcgtcga gcagcgggtg ggtcgttttg tgcgccggaa gaagatcgaa cttaggatag
      421 ccgacaatcg agatcttgcc cggatctacg cccgtctcgc caatcagacg gtcgcggatt
      481 ttgcgcccgg acaccagaac gtggtcaaag cccgcactgg ccttgtcgaa gccgatggcg
      541 cggtcccccg cgccatggcg cgtgtggacg atcttgaggt cgccaaggcc gtagcggctc
      601 ttcagtatca gcgaggtctt ctcggcgacc accagaatgt ccagcgatct gaagaaatcg
      661 aggttgtcgc tgtagaccag cagcttgctg gccggcagaa ccgcctcggt cgcggcggcc
      721 agaagtttgg acgaccggcg ggtcaggccc agttcgacta gcgtcaggcc ctgtccgatc
      781 aggccgccgc ccagccggat gacctcggcg gtcagccgtc ggttggtggt ggcgaccacg
      841 atctccacgc cttgggcgcc gtgggccaag gccaaggcga tcggcaggct atgggccacc
      901 tgatggactt ggtcgtgatt gaagaggaag ccgatgcgca tggcccaacg acgtcatgcg
      961 gcgcaccctc cctcaccgat tttccgacca gctgacgaac ggcgttcaag cggctctgca
     1021 cggaggggcg gcttgctcga caattctcgc tttgaggcaa tccatcaacg ccccgagcgt
     1081 ggcgcaatcg gcgccgacca ggtcgtcgat ctcgacgtca aacctatcct cgatcgcgac
     1141 cagcacggcc atataggcgt ccagacgcag gccaaggtcg cagatgcggg tcgaacgctg
     1201 caacacagaa cgcgcgaggc ccgatttgct ggcgatcagg ctggcgatat cgtcatcggt
     1261 gagaagggcg tcgatcatag caaggggctc ccacgtgggc tctggctgct tgacgccatg
     1321 acacgcccgc cccctcaccg attaagcgcg cactcgcccg tcctccatcc gcagcacccg
     1381 gtcggctaga tcggcaagcc gcccgtcgtg ggtcacgcag acgaccgtcg cgccgctggc
     1441 ctgcgcctgc gcgcgcagta ggccggcgat ctggcggcca ttttcggaat ccagggccga
     1501 ggtcggttcg tcggcgaaga tcagggccgg ccgtttggcc agggctcggg ctatggcgac
     1561 ccgctgcttt tcaccgccag acatctcacc ggggcggctg gccagccgcc gccccagccc
     1621 caccgcctcc agggcctcga tggctcgcga ccgcgcctct cgggccgaga cgccgagttg
     1681 acgcaaaacg atttcaacct gctcgctggc ggtcagggcc ggcagcaaat ggaagccctg
     1741 gaatatgaag cccgagtacg ccaagcgaaa gcgatcgcgc tggtcggcgc tcatgacctg
     1801 gagcggccgt cccaacgcct cgaccacacc ggcgtcggga cttagcaggc cggaaatgat
     1861 ggctagcagg gtcgactttc cagaaccgga cggaccggtg atcatcacca gttcgccggg
     1921 ccgcaggctc aagctgacgg gatggagcgc atcgacctgt tgcacgcccg cgccatagcg
     1981 cttgcacacg gcctgcgcga cgagcgaggg gctcatgaaa cgccgcccag cgccaaggaa
     2041 aggtcgatat aggcctgagc gcgcgcggcc cgggcatcgg ccagggtggt atctgcggcg
     2101 gcggcccgta cctccagcgc cgcaagggcc acgccgtcga tgtcgccgtc gcgatgcagg
     2161 gtctggcccg tcccgaacgc caaaacatcc tgtcgccaac gcttgcgggc cagggcctcg
     2221 gccgtcgtgg cgtcctgcag cgcagccagc gccagccgcg tctcgcccca ggcttctccg
     2281 accgccttgc gataggctag ctcggcgagc gccgcgtcgg ccgtcgcgcc gcgcttgcgc
     2341 gccttggcgg caccgccgtc gaaaagttga tgggtcaggc cgccggccac ggcccaggct
     2401 agggcccgca cgtccagaag atacagcagg tcggtgtccc ctgaaccgat gccggcggtc
     2461 agcatgaagc gcggccgcga cgcggcgacg gcttcggcgc ggcgcgcgtc ggcggcgcgc
     2521 aggcgggcct gggcggcgag gatatcggcg cgacccagaa ccgcctcgga cggcacggcg
     2581 gttataggtg gggttagggg aatttcatca ccggcgtagg ctggttcgtc gatgggcgcg
     2641 tcctcgccca gcaatatgcc cagtcgcgtt cgttcggcct cgaccgcatg cgtcgcacgt
     2701 tgaagctcgc cgcccgcctg gtcctcctgg gcccgcgcgg ctaccacctc agcgctagtg
     2761 gcggcgccat cggcctgacg gcgccggatc agatcgctaa cctgacccac caaaggcttc
     2821 aggacttccg ccgccgcttg atgggcctgg gcggcgcgca aggcgacata ggcgtgcgcg
     2881 acctccgcca aaacccggcg tcgaacgccg gcggcgtcct gcaggctggc ggcctcgtcg
     2941 gccatcgccg cgtcctggcc gtgcttcaat cgcccgaata tatcgacttc gtagctggct
     3001 tggagcggtg ttcctgcgcc acttcgcacg gaagagaagt ctgcgccccc gttcgccagc
     3061 tctgcgccaa ccgtcagatg cggtcgccgc ccggctcggg ccagatcaac atccgcgccc
     3121 gcccgctcca acctggctaa ggccattttc aggtccagcg agcccagctc ggcccgaacc
     3181 aacaggtcgg ccagcaccgg atctccaaga tccggcgacc acggggagcc
//
LOCUS       DAOMEO010000255         3228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3349_length_3228_cov_2.614245, whole genome shotgun sequence.
ACCESSION   DAOMEO010000255 DAOMEO010000000
VERSION     DAOMEO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3349_length_3228_cov_2.614245"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLH31_07080"
     CDS             complement(<1..453)
                     /locus_tag="PLH31_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQR89016.1"
                     /translation="MTTMADGAIDHTVQIERVNRVGWIVLSRPAQINAINPALRREVM
                     QALEVLDADPDVGAIVIRGAGERGFCAGADIKAQSDAASLPALRAETTPGWIEALDRV
                     RKPVIAAIHGYCLGGGLEISMACDIRIASPNAVFGLPETGLGLIPGGGG"
     assembly_gap    584..593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<594..1784)
                     /locus_tag="PLH31_07085"
     CDS             complement(<594..1784)
                     /locus_tag="PLH31_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89017.1"
                     /translation="MPNTSTTPLAKGAWYTLVILTLVYVSNSIDRTAMSILIEPVKAE
                     FKLSDSQLGLLTGLAFGLTYALTGLPMGWLIDRVNRTRLLATVVAVWSLCTAICGFAQ
                     NYPALVMARLAVGASESAAAPTAMSMIADLFPKNRRSTAMGVFWTSTAFGTAISLVLG
                     GVIAANYGWRTAFFVAGAPGLILAILIILTVREPVRERDIGQDDQTPAPSLLQTMRFV
                     CANPTVFHAFVGIGLASLAMSGVPVWAASFLVRTQGFTLPQAGLMAGLGVGLFGALGS
                     FLGGPAGDAVVRRWGVQALPAAPMVACVLACVSGLIFALGSSLMVVALGFIVFEIVSR
                     GFTAPAYAILVTGVEPRMRGVVVSAVQAVTNLVGYGVGPLVVGVVSDRVGGTNSLKVG
                     IAAVM"
     gene            2026..3048
                     /locus_tag="PLH31_07090"
     CDS             2026..3048
                     /locus_tag="PLH31_07090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HQR89018.1"
                     /translation="MRALLSLQAGGPETLRIREIDEPSPGPGQVAVTVHACGVNFPDV
                     LVIEDRYQLRPPRPFSPGSELAGVVRAVGEGVASPGVGDRVSASLPFGAMAEVVIVSA
                     DRCTVVPGAMPFDEAAAFQVTYGTVYHALVGRAGLRQGETLLVLGAGGGIGLAAVELG
                     KALGARVVAAASSQDKLDAALAMGADAVVLYPRAFEDVGQARAFTEAVRAACGGEGAD
                     IVIDPVGGAYAEPAFRAIAGQGRYLVVGFAAGIPAIPLNLVLLKAAQIIGVFWGAYMA
                     SHPQAGARDMGVLLDLYVAGKIRPCVSERYSLERGGEAIARLGSRGVSGKIVVDVQSS
                     ESRTNA"
BASE COUNT          572 a          995 c         1133 g          518 t
ORIGIN      
        1 gccgccgccg cccgggatca ggccaagtcc cgtctcgggc aggccgaaca cggcgttcgg
       61 tgatgcgatc cgaatatcgc aagccatgga gatctccagg ccgccgccca ggcaatagcc
      121 atggatggcg gcgatcaccg gcttgcggac ccggtccaag gcttcgatcc accctggtgt
      181 cgtctcggcg cgaagcgccg gcaaggacgc cgcgtcgctc tgggccttga tgtcggcgcc
      241 agcgcagaag ccgcgttcgc cggctccccg aatgacgatc gcgccgacgt cggggtcggc
      301 gtccaacact tccagcgcct gcatcacctc gcgacgaagg gcgggattga tcgcgttgat
      361 ctgtgcaggg cgactaagaa cgatccagcc cacgcgattg acgcgttcga tctggacggt
      421 gtggtcgatg gctccatctg ccatggtcgt catgctccga cgttaccggc ggacggcgct
      481 cgaccgccga cggtgtgctg ggaattgcca ggaagaaggc tcatgcggtc gctccgtcga
      541 cgaagcgttc ggaccggcgc gcggcgagcc aggcggcgag gaannnnnnn nnncatcacc
      601 gcggcgatgc cgaccttcag ggaattggtt cctccaacgc gatcgctgac aacgcccacg
      661 accagaggac cgacgccgta gcccacgaga ttggtcacgg cctgaaccgc ggagacgaca
      721 acgcctctca tgcgcggctc cacgccggtg acgagaatgg cgtaggcggg ggccgtaaag
      781 ccgcgcgaga cgatctcgaa gacgataaag ccgagggcga cgaccatgag cgacgatccc
      841 agagcgaaga taaggcccga gacgcaagcc aggacgcaag cgaccatagg cgcggccggc
      901 aaggcctgga cgccccaacg gcggaccacg gcgtccccgg ccgggccgcc caagaaggac
      961 cccaacgcgc cgaacagtcc gacgcccagg cccgccatca ggccggcctg gggcagggtg
     1021 aaaccctgcg tgcggaccag gaaggatgcc gcccagaccg gcacgcccga catggccaac
     1081 gaggccaagc ctatgccgac gaaggcgtga aagaccgtcg gattggcgca gacgaaccgc
     1141 atagtctgca atagggacgg agctggcgtc tggtcgtctt ggccgatatc gcgctcccgg
     1201 acgggctcgc ggacggtcag gataatcagg atggcgagaa tcagcccagg ggcgccggcg
     1261 acgaagaagg cggttcgcca accgtagttg gccgcgatca ccccgccgag cacaaggctg
     1321 atggccgtgc cgaaagccgt gctggtccag aatacgccca tggcggtcga gcgccggttc
     1381 ttgggaaaca ggtcggcgat catcgacatc gccgttggcg ccgccgccga ttccgaggct
     1441 ccgaccgcca gccgcgccat gaccaacgcc gggtagtttt gcgcgaagcc gcaaatggcg
     1501 gtgcaaaggc tccagaccgc gacgacggtc gcaagcaacc gggtccggtt gacccggtcg
     1561 atcagccatc ccatcggcag gcccgtcagc gcataggtca gaccgaacgc gagaccggtc
     1621 agcaggccca actggctgtc ggagagcttg aactcggctt tcacgggctc gatcaggatg
     1681 gacatcgccg tccggtcgat ggagttcgac acatagacga gcgtcaaaat gaccagagtg
     1741 taccaagcgc ccttggcgag cggcgtggtg gaggtgttgg gcatggctag ggccagaccc
     1801 tgctgcccag gctccaggtc gccgaggtgc ggaaaatgac gggcactgat atcctccggg
     1861 cgcgctttga actgcgtctc tttcgtcgag gctagagtag cgccaataat tcggcaagcg
     1921 aaacgaattt ctggtatcag aataatatga ttgtcgctcg ccggcctggt gactagaaaa
     1981 ggtcggcgga tcgatatggg ttcgcgcctt tcccgcgagg tccccgtgcg agcccttctg
     2041 agtctgcagg ccggtggccc ggaaaccctg agaatccgcg agatcgacga gccctcgccg
     2101 ggcccgggtc aggtcgccgt gaccgtacac gcctgcgggg tcaatttccc cgatgtgctg
     2161 gtcatcgagg atagatatca gctgcggccg cccaggccgt tttcgcccgg cagcgagctg
     2221 gcgggcgtgg tgcgcgcggt cggcgagggc gtcgcgtcgc cgggcgtggg cgatcgggtc
     2281 tccgcgtcgc tgccgttcgg cgcgatggcg gaagtcgtca ttgtgtcggc ggatcggtgc
     2341 acggtcgtgc cgggcgccat gccgttcgac gaagcggcgg cgttccaggt gacctacggc
     2401 accgtctatc atgctctggt ggggcgtgcg ggtctgcgac aaggcgagac gctgctggtt
     2461 cttggcgccg ggggcggcat tgggcttgcg gccgtggagt tgggcaaggc cctgggcgct
     2521 cgcgtggtcg cggccgcctc ttcacaggac aagctggacg cggcgctggc gatgggcgcc
     2581 gatgccgtgg tgttgtatcc gcgcgcattc gaggatgtcg gtcaggccag ggccttcacc
     2641 gaggcggtgc gggctgcttg cggcggggag ggggccgata ttgtcatcga cccggtcggg
     2701 ggggcctatg ccgagccggc gttccgggcc atcgccgggc aagggcgcta tctggttgtg
     2761 ggtttcgcgg ccggcattcc cgccattccg ctcaatctcg tcctactcaa ggcggcccag
     2821 atcatcggtg tcttctgggg cgcatacatg gcgagccacc cgcaggccgg cgcgcgggac
     2881 atgggggtgt tgttggacct gtatgtcgcg ggcaagatca gaccctgtgt ctccgaacgc
     2941 tattcgctgg agcggggcgg ggaagcgatc gcgcggttgg ggtcgcgcgg cgtctccggc
     3001 aagatcgtcg tggatgtgca aagttctgaa agcagaacga atgcctaata tcagaattat
     3061 gctggaccga tcgggcaggc ccgactaatt tgtgacgaag tgaccttcgc actacgaagg
     3121 cgggttcctg cctggcgctc acacccactg gatgggcgtc ggcaggagcg ctggggacga
     3181 cattcgaggc ctgtcaccgc tgttgccttt gggatgatcc ggagcgac
//
LOCUS       DAOMEO010000256         3227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3350_length_3227_cov_2.562421, whole genome shotgun sequence.
ACCESSION   DAOMEO010000256 DAOMEO010000000
VERSION     DAOMEO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_3227_cov_2.562421"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="PLH31_07095"
     CDS             complement(<1..2073)
                     /locus_tag="PLH31_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HQR89019.1"
                     /translation="MTLTALASPAPRETPASPTHRALALCRAADTLLPLLVQGVPVDA
                     RALRTAMEAAFQGSDAQGFWDWKDAYEACEAAQLLFLRRFGSALMRQSSAAQLAMISK
                     IAGLLPSQTRRSLESEALQQFSTPIPVAWIAARAAGLGAGDLVLEPSAGTGLLAIFAE
                     IAGAELALNELGDGRAELLGRLFPATPVSRFDAAHIHDHLDAAVIPTVVLMNPPFSAG
                     AHVEGRVADAALRHIASALARLADGGRLVAITGAGLSPHNPAWRDAFSRLQQRGRILF
                     SAVIAGSVYARHGTTTETRLTIIDRTPAEDPDRFPDSPGMAANAAQLLTWVDAHVPPR
                     AVVTPAHPTRRLCPKPALHRAASAARRPAPPPAQTSGRPLGYTVCDWSPPVGDRLTEA
                     LYEPYVLQSIAIEDAKPHPTPLVQSASMASVCPPKPRYVPLLPDGLVAAGLLSDAQLE
                     TVIYAGETHDGHLAGAWSVDATFDQVSAAADDTEGAVRFRRGFMLGDGTGAGKGRQVA
                     GIILDNWLKGRRRAVWVSKSDALIEDAQRDWSALGQEGLLVTPLSRFRQGAPIRLDEG
                     ILFATYATLRSDERDGKVSRVRQIVDWLGADFDGVIVFDESHAMQNAAGGKGERGDQA
                     ASQQGKAGLRLQHALPGARILYVSATGATTVHNLAYAQRLGLWGGDDFPFATRAEFVE
                     AIEAGGVAA"
     gene            complement(2161..2403)
                     /locus_tag="PLH31_07100"
     CDS             complement(2161..2403)
                     /locus_tag="PLH31_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6117 family protein"
                     /protein_id="HQR89020.1"
                     /translation="MAIPHHASRNFQTLLQAARNDNLALMECLDVETRELRYVLCAVG
                     RDGEDFVFTPFGHLAPGNPFEAYLPPGEGGGFVSPE"
     gene            complement(2495..>3227)
                     /locus_tag="PLH31_07105"
     CDS             complement(2495..>3227)
                     /locus_tag="PLH31_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR89021.1"
                     /translation="TLALRDAVGGDPDTAYLAVLHALALKTFYRFSTATCLELEVKHM
                     TFGHQAADLNETASAKAIAERHARWAEQLPKEPGALWQTLVGFDAESRQALFAHCVGL
                     SVNAVHQSWNQGERMAHADALARAVDLDMVAAGWTPTAETYLGRVTKTRILEAVREGK
                     GEREAQLIEHLKKGDMAKEAERLLAGSGWLPEPLRTADDQTLVEAEETAEPEALPAFL
                     TDDEDEDAADEPNAAEPGFHAVAAE"
BASE COUNT          489 a         1053 c         1210 g          475 t
ORIGIN      
        1 cgccgccacg ccgccggcct cgatcgcctc gacgaactcg gcgcgggtgg cgaaggggaa
       61 atcgtcgccg ccccaaaggc cgagccgctg ggcgtaggcg aggttgtgaa ccgtggtggc
      121 cccggtcgcc gagacgtaga ggatgcgggc gcccggcagg gcgtgctgca ggcgcaggcc
      181 agccttgccc tgctgcgagg cggcttggtc gccccgctcg cccttgccgc cggcggcgtt
      241 ctgcatggcg tgggattcgt cgaacacgat gaccccgtcg aagtcggccc ccaaccaatc
      301 gacgatctgg cggacccgcg aaaccttacc gtcgcgctcg tcggaccgta gcgtggcgta
      361 ggtggcgaat aggatgcctt cgtccagccg gatcggagcg ccctggcgaa agcgcgagag
      421 gggcgtgacg agcagcccct cctggccaag cgccgaccag tcacgctggg cgtcctcgat
      481 gagcgcgtcg gatttggaca cccagaccgc gcgacgccgg cccttcaacc agttgtcgag
      541 gatgatgcca gcgacctggc ggcccttgcc ggcgccggtg ccgtcgccga gcatgaagcc
      601 gcgacggaaa cgcaccgcgc cctcggtgtc atcggccgcg gcgctgacct ggtcgaaggt
      661 cgcatcgacg ctccaggcgc cggccagatg gccgtcatgg gtctcgccgg catatattac
      721 ggtttcgagc tgcgcgtccg acaacagacc agccgcgacc agtccgtccg gcaggagcgg
      781 gacgtagcgc ggcttgggcg ggcagactga cgccatcgat gccgattgca ccagcggggt
      841 gggatgcggc ttggcgtcct cgattgcgat cgattgcagg acatagggtt catagagcgc
      901 ttcggtcagc cggtcgccga caggcgggct ccagtcgcac acggtgtagc caagcggccg
      961 gccgctggtc tgcgccggtg gcggagccgg tcggcgcgcc gccgaagcgg ctctgtgcaa
     1021 agccggtttt gggcaaagcc ggcgcgtcgg gtgcgcgggc gtgacgacgg cgcgcggcgg
     1081 cacatgagcg tccacccagg tcagcaactg cgcggcgttg gcggccatgc cgggcgagtc
     1141 gggaaagcgg tcgggatcct cggccggcgt ccggtcgatg atcgtcaggc gcgtttcggt
     1201 cgtggtccca tgccgcgcat agacagatcc ggcgatgacc gcggagaaca ggatgcggcc
     1261 gcgctgctgg agacggctga aggcgtcgcg ccaggccggg ttatgaggcg acagacccgc
     1321 gccggtgatg gcgaccaagc ggcccccatc ggccaggcgc gccagagccg acgcaatgtg
     1381 acgcagggcg gcgtcggcga cccggccctc aacatgggcc ccagccgaga agggcgggtt
     1441 catcaggacg acggtgggga tcaccgcagc gtcgaggtgg tcgtggatgt gggctgcgtc
     1501 gaaccggctg acaggcgtgg cgggaaacag gcgaccgagc agttcggcgc gcccatcgcc
     1561 cagttcgttg agcgccagct cggcgcccgc gatctcggcg aagatggcga ggaggccggt
     1621 tccggccgaa ggttcgagca caaggtctcc ggctccgagt cccgcggcgc gcgcggcgat
     1681 ccaggcgacc gggatcggtg tcgagaattg ctggagcgct tcgctttcca gcgaccgccg
     1741 cgtctggctg ggcaggagcc cggcgatctt gctgatcatg gccagctgag cggctgagga
     1801 ctgacgcatc agggctgatc cgaaccggcg caggaacaga agctgcgcgg cctcgcaggc
     1861 ttcataggcg tccttccagt cccagaaccc ttgcgcgtcg gagccctgga acgccgcctc
     1921 catggcggtg cgcaaggcgc gcgcgtcgac gggaacgccc tgaacgagca gcggcaggag
     1981 ggtgtcggcg gcgcggcaaa gcgccaaggc gcggtgggtc gggctggcag gcgtctcgcg
     2041 cggcgccggc gaagccaggg cggtcagggt catgttcggt catccaggag agcgggagga
     2101 aggcgagccg aacggcgctc tctctcgacc gcaccggctc gccccctccc ggccccgctc
     2161 tcactctggc gagacgaagc ctccgccctc gccgggcggg agataggcct cgaagggatt
     2221 gccgggggcg agatggccga agggcgtgaa cacgaagtcc tcgccgtcgc ggccgacggc
     2281 gcacaggacg tagcgcagct cgcgggtttc gacgtcgagg cattccatca gggcgagatt
     2341 gtcgttgcga gccgcctgga ggagggtctg gaagttgcgg gaggcgtggt gtgggatggc
     2401 catggcggac tcctgaagcg caaagcggcc ccgccatcgg cgggccatga ggggaagagc
     2461 aggtgcgggg cggcgagcgc cgccccgcgg tcgctcattc ggcggcgacg gcgtgaaagc
     2521 ccggctcggc cgcgttcggc tcgtccgccg cgtcctcatc ctcgtcatcg gtcaggaagg
     2581 ccggcagcgc ctcgggttcg gcggtttcct cggcctcgac gagcgtctgg tcgtcagcgg
     2641 ttcgcagcgg ctcaggcaac cagcccgatc cggccaggag ccgttcggct tccttggcca
     2701 tgtcgccctt cttgagatgc tcgatgagct gggcttcgcg ttcgcccttg ccttcgcgca
     2761 cggcttccag aatccgggtc ttggtcactc ggcccagata ggtctcggcg gtcggcgtcc
     2821 agcccgccgc gaccatgtcg agatcgacgg cgcgggcgag cgcatcggcg tgggccatgc
     2881 gttcgccctg gttccaggac tggtggacgg cgttgacgct gagcccgacg cagtgggcga
     2941 acagggcttg ccggctttcg gcgtcaaacc cgaccagggt ctgccaaagc gcgcccggct
     3001 ccttgggaag ctgctcggcc cagcgcgcgt gacgttcggc gatcgccttg gcggaggccg
     3061 tctcgttcag atccgcggcc tgatggccga aggtcatatg cttgacctcc agctccaggc
     3121 aggtggcggt gctgaaacga tagaaggtct tcagcgccag ggcgtggagc acggcgagat
     3181 aggcggtgtc gggatccccg ccgacggcat cgcgcagcgc cagcgtc
//
LOCUS       DAOMEO010000257         3225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3354_length_3225_cov_2.160252, whole genome shotgun sequence.
ACCESSION   DAOMEO010000257 DAOMEO010000000
VERSION     DAOMEO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_3225_cov_2.160252"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="PLH31_07110"
     CDS             complement(<1..125)
                     /locus_tag="PLH31_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQR89022.1"
                     /translation="MADLRRPERTFTIRNLCNEFKATPRALRFYEDKGLLNPAREG"
     gene            316..957
                     /locus_tag="PLH31_07115"
     CDS             316..957
                     /locus_tag="PLH31_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89023.1"
                     /translation="MTALSHTEIWTAIDALAKRFDMSPSAMAKMAGLDPTSFNRSKRT
                     SSDAEARPRWPSTESLAKVLEATGVGFSEFAALTETAKRPPARGVPLLGFAQAGSDGF
                     FDDAGFPAGEGWDEIAFPGLQEDGVYALEISGDSMVPVFRDGDRILVSPSIEPRRGDR
                     VVVKTNDGEVMAKELSRVTARTIELTSLNRDYDDRVLERGDVAWIARILWASQ"
     gene            complement(974..1183)
                     /locus_tag="PLH31_07120"
     CDS             complement(974..1183)
                     /locus_tag="PLH31_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89024.1"
                     /translation="MASFTLRPSDRSEFDSLLGDLTSKFPGAHVEAAHNDIWGSYRVD
                     VSCDAPWEGDLLAYINGQHGNCPRT"
     gene            complement(1440..3095)
                     /locus_tag="PLH31_07125"
     CDS             complement(1440..3095)
                     /locus_tag="PLH31_07125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQR89025.1"
                     /translation="MFEGLSPLARDARAWPFEQARALLARTLRLRLSDAERDLAATLI
                     HAGKTDEAVATLEALRKPVVLETGYGPSGLPHMGTFGEVARTTMVRAAFRALTDDKIP
                     TRLISFSDDMDGLRKIPPNVPNGEVMIPDLDKPLTVVRDPYGEFESFGHHNNARLRAF
                     LDGFGFDYEFVSSTDCYRGGRFDETLLIALERFDAIQKIMLPSLGEERRATYSPFLPI
                     SPKTGKVLQVPTLERNVAKGTIVFRDEDGELTEVPVTGGGVKMQWRPDWAMRWTALGV
                     DYEMSGKDLIDSVRLSNQVCKVLGGSPPEGFHYELFMDENNQKISKTKGNGLTMDEWL
                     RYGAPESLAYYMFQSPKSAKKLYFDVIPKATDEYLQQLDAYPKQEPAKQIDNPAWHVH
                     SGQPPQQGSPVSFSLMLNLVSAADASTKDILWGFLGRYVDGATPQTQPLLDRLAGYAI
                     NYYEDFVKPSKTFRAPTDVERAAMVDLRTRLAALPADCQDAELIQNEVFAVGKDHSFD
                     PLRAWFQALYEVLLGQSQGPRFGSFAAIFGLDRTIALIDEKLS"
BASE COUNT          552 a          985 c         1098 g          590 t
ORIGIN      
        1 ccctcgcggg ccggattgag caggcccttg tcctcataga agcgcagcgc gcggggcgtg
       61 gctttgaatt cgttgcagag attgcggatc gtgaaggtgc gctcggggcg acgcaggtcg
      121 gccaacatag aaacctccca agaggaaagg cctcgctaca cggcgaggcc gctttgttat
      181 cgttgatcac cctattccgg gtttccgttg acgtcaacgt ttacgtaaac gtcaacttga
      241 cggtggcgag ataggtttac tttcctaaag gccaggctta ttttcctgtt ctcaactccg
      301 ccgggatttc gcaacatgac ggcgctttcg cataccgaaa tctggaccgc gatcgacgcg
      361 ctcgcaaagc gtttcgacat gagcccctcg gccatggcca agatggcggg tctggacccc
      421 acaagcttca accgctccaa gcgcacctcg tccgacgccg aggcgcggcc gcgctggccc
      481 tcgaccgaga gcctggccaa ggtgctggag gccaccggcg tgggcttttc cgagttcgcc
      541 gccctgaccg agacagccaa gcgcccgccg gcgcgcggcg tgccgctgct gggtttcgcg
      601 caagccgggt cggacggctt cttcgacgat gcgggctttc ccgccggcga gggctgggac
      661 gagatcgcct ttcccggcct gcaggaagac ggcgtctatg cgctggagat ttcgggggac
      721 tcgatggtcc cggtgtttcg cgacggcgac cgcatcctgg tctcgccctc gatcgaaccg
      781 cgccgcggcg accgggtggt ggtcaagacc aacgacggcg aggtgatggc caaggagtta
      841 tcgcgggtca cggcccggac gatcgagctg acctcgctca atcgcgacta tgacgaccgt
      901 gtgctggagc gcggcgatgt ggcctggatc gcccgcattc tctgggccag ccagtagacc
      961 gcagacttaa agtctaggtg cgcgggcagt tgccgtgttg cccattgata taggccagaa
     1021 gatcgccctc ccacggcgcg tcgcaggaga cgtcgacccg gtaggagccc cagatgtcgt
     1081 tgtgcgcggc ttccacatgg gcgccgggga acttggaggt cagatctccg agcagagaat
     1141 cgaattccga acggtctgac gggcgaaggg tgaacgaagc cattggacta accttggggt
     1201 tctgatcctg gaaccttacg ccgacaattg cggacattcc cttaaccggg aattcagact
     1261 tcgttcatct tagcggacat ggtgtcgcag gggtgaggac tcaccgaggg cgtgtaggca
     1321 gggccttagc tgctccccct ctgggggagc tgtcgcggag cgactgaggg ggctaatgcg
     1381 gcctaagccc agctgacccc ctccggccct ctgggccacc tcccccagag ggggaggatc
     1441 taggagagct tctcgtcgat cagcgcaatc gtccggtcca ggccgaagat cgccgcgaag
     1501 gagccaaagc gtgggccctg gctctggccc agcagcactt catacagagc ctggaaccag
     1561 gcccgcagag ggtcaaagct gtggtccttg ccgactgcga acacctcgtt ctggatcagc
     1621 tcggcgtcct ggcaatcggc cggcagcgcg gccaggcggg tgcgaagatc gaccatcgcc
     1681 gcgcgctcca catccgtcgg ggcgcggaag gtcttcgagg gcttcacgaa gtcctcgtag
     1741 tagttgatcg catagccggc caggcggtcc agcagcggct gggtctgcgg cgttgcgccg
     1801 tccacgtaac ggcccaggaa cccccacagg atgtccttgg tcgaggcgtc ggcggccgag
     1861 acgagattca gcatcaggct gaacgacacc ggcgagccct gctgcggcgg ctggcccgaa
     1921 tgaacatgcc aggccgggtt gtcgatctgc ttggccggct cctgcttcgg ataagcgtcc
     1981 agctgctgga gatattcgtc cgtcgccttg gggatgacgt cgaaatagag cttcttggcc
     2041 gacttggggc tctggaacat gtagtaggcc aggctctcgg gcgcgccata gcgcagccac
     2101 tcgtccatgg tcaggccgtt gcccttggtc ttggagatct tctgattgtt ctcgtccatg
     2161 aagagttcgt agtggaaccc ttccgggggc gagccgccca gcaccttgca cacctggttg
     2221 gacaggcgga cgctgtcgat caggtccttg ccgctcatct cgtagtcgac gcccagggcc
     2281 gtccagcgca tggcccagtc gggacgccac tgcatcttga cgccgccgcc ggtcaccgga
     2341 acctcggtca gctcgccgtc ctcgtcgcgg aacacgatcg tgcccttggc gacattgcgc
     2401 tccagggtcg gaacctgcag caccttgccg gtcttggggc tgatcggcag gaacggcgaa
     2461 taggtggccc ggcgctcctc gcccagtgag ggcagcatga tcttctggat ggcgtcgaaa
     2521 cgctcgagcg cgatcagcag ggtctcgtcg aaccggccgc cacgatagca gtcggtagac
     2581 gacacgaact cgtagtcgaa accgaagcca tcgaggaagg cgcgcaggcg ggcgttgttg
     2641 tgatggccga agctttcgaa ctcgccatag gggtcgcgga ccacggtcag cggcttgtcg
     2701 aggtcgggga tcatgacctc gccattgggc acgttcggcg ggatcttgcg caggccgtcc
     2761 atgtcgtcgg agaacgagat caggcgcgtg gggatcttgt cgtcggtcag ggcgcggaac
     2821 gccgcgcgga ccatggtcgt gcgcgccacc tcgccgaagg tgcccatgtg cggcaggccc
     2881 gacggaccat agccggtctc cagcaccacc ggtttgcgca aggcctccag cgtggcgacc
     2941 gcttcgtcgg tcttgccggc gtggatcagg gtcgcggcga gatcgcgctc ggcgtcggac
     3001 aggcgcagac gcagcgtgcg ggccagcagg gcgcgcgcct gctcgaacgg ccaggcgcgt
     3061 gcgtcgcggg cgagggggga aaggccttca aacatgcggg cggtataggg cgaagcggcg
     3121 gtggtggcta ggttttgggt gaggattgga gggtgggtct tatgcttggg cctgagttgc
     3181 tttccaactc cgaaccaatg ggaggcgctc gattggtcgg ttacc
//
LOCUS       DAOMEO010000258         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3364_length_3217_cov_3.023719, whole genome shotgun sequence.
ACCESSION   DAOMEO010000258 DAOMEO010000000
VERSION     DAOMEO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_3217_cov_3.023719"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLH31_07130"
     CDS             <1..1315
                     /locus_tag="PLH31_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89026.1"
                     /translation="IPIYMAILFFIDPIIGLVTFLGGMTLVGLAFLQDRATRPLTKAA
                     NSAAIKSYGFTEAGLRNSEVVRAMGMVGYLGRQWGKFRAESLTNAKSSNEQAEIWGGL
                     IKFVRIAVQILIIAIGADLILNHKIGSGMLFANMILSARALQPIDRVVGTWTSLIGAS
                     QAYERLSGLLNNYTPSALGTSLPRPKGDLTVENINYGVPGSGRLLLAAVNFRIAPGEF
                     LGIVGPSGAGKSTLARLLVGVQQPLNGSVRLDGSDVFGWSRAEFGQYVGYLPQDTELF
                     AGSVRDNIARFMDGVTDDEVIAAAQAANVHELIVRLPNGYDTELGQGGVVLSVGQRQR
                     VGLARAMLRNPALIVLDEPNANLDAQGEEALLEALAAMKKRAQTVVVVSHKPSMLVGA
                     DKLLVLRDGRVDLFGPREAVLARLAQNAAKAKGPNASAPAIEAKS"
     gene            1312..2700
                     /locus_tag="PLH31_07135"
     CDS             1312..2700
                     /locus_tag="PLH31_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HQR89027.1"
                     /translation="MKYDLKRLTPWISPDVPAEPPSIGGPVDAPLSEGARQNLRKPIV
                     LGLAVIGVFVFGLGAWAAISQIRGAVTAPGVFRVEASRKTLKSRDGGLVRQINVREGD
                     AVAVGQLLLKFDDTVPKAQVDIYENQYDAALMQAARLRAEIVRRPLIVPPELQARRTD
                     PRVSEIIQNEMTVYDVRRAAIESQAAILNQRFEQLQSARAGLQIQADSAVEQIALSEE
                     ELEGYKTLLAKGYAPKTLVLRLERQLSESKARRGSLMAEITRNSQQSGETRLQLSSLY
                     EQRASESASNLRDVEARITDLGPRLGAAREALAQTEIRAPAAGYVLGLSQHTIGGVAA
                     PGEVLMDVVPSNAPLVITAEVRPSDIDEVQPGMQAQVALQAYSSFRVPKIQAEVLTVS
                     ADALANAETKSSYYRVDLRISPEELRKLPKGVRLSPGMQVSVMIMTGRRTILSYLLGP
                     IGEVIDQSMREQ"
     gene            2709..>3217
                     /locus_tag="PLH31_07140"
     CDS             2709..>3217
                     /locus_tag="PLH31_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89028.1"
                     /translation="MTLAELKQPKVDFLVAGVQKGGTTALFDYLREHPDLLLADVKEV
                     HFFDNENVDWQKPDYESYHAHFHPPADGRRRLWGEVTPIYTYWPRCFERIADYNSAMK
                     LVLVFRNPIERAWSHWRMEYARGAETEPFAWCIREGRHRVNGDPSAPGHHREFSYVER
                     GFYGAQVQRL"
BASE COUNT          644 a          916 c          969 g          688 t
ORIGIN      
        1 gataccaatc tacatggcga ttttgttctt catcgatccg atcattggtc tggtcacgtt
       61 cctcggcggc atgactctag tcggattggc ttttcttcaa gatcgcgcca cccgcccttt
      121 gaccaaggcc gctaatagcg ccgccattaa gagctatggc ttcacggaag cgggtcttcg
      181 caatagtgaa gttgttcggg ccatggggat ggtaggctac ctcggccggc aatggggaaa
      241 gttccgcgct gagtccttga ccaatgccaa gagttcaaac gaacaagctg aaatctgggg
      301 cgggcttatc aaatttgtcc gtatcgcggt tcaaattttg atcatcgcga tcggagcgga
      361 tcttattctg aaccataaaa ttggttcggg tatgttgttt gcgaacatga tcctttcagc
      421 gcgagccttg cagcctatcg accgggttgt agggacctgg acctcactta tcggcgcaag
      481 ccaagcttac gagcgtctct ctgggcttct gaacaactat acgccgtccg cgcttgggac
      541 gagcctgccg cgtcccaaag gcgatctgac tgttgaaaac atcaactatg gcgtgccggg
      601 atctggtcgc ctgctgcttg ctgcggtcaa tttcagaatt gcgccgggtg agtttcttgg
      661 gatcgtaggg ccatcgggag caggcaaatc cactctggcc cgccttctcg taggcgttca
      721 gcagcccttg aacggttcgg ttcggctgga tggttcagac gtgtttggct ggtcccgggc
      781 cgaattcggc cagtatgtcg gttacctgcc acaagacacc gagctttttg cgggcagtgt
      841 gcgcgataac attgcgcggt tcatggatgg cgttaccgac gatgaagtga tcgcggcggc
      901 gcaggccgcc aatgtccatg agcttatcgt gcgactgcca aatggatatg acaccgagct
      961 gggtcagggc ggcgtcgtcc tgtcagtcgg tcaacgacag cgtgtcggcc tggcgcgagc
     1021 catgttgcgc aatcccgcct tgatcgtttt ggacgaaccc aacgccaatc tcgacgccca
     1081 gggcgaagag gcccttttgg aagcgttggc ggcgatgaag aagcgcgccc agaccgtcgt
     1141 tgttgtctcc cacaagccca gcatgttggt gggcgctgac aagctgctcg ttctcaggga
     1201 tgggcgagtt gatctcttcg gtcccaggga agcggttctg gcccgattgg cccagaacgc
     1261 cgctaaggcg aaagggccaa atgcgtcggc cccggctatc gaggccaagt catgaagtat
     1321 gatctcaagc gtttgacccc gtggatttcg cccgatgttc ccgctgaacc gccatcgatc
     1381 ggcggccccg tcgatgcgcc cttgtccgaa ggggcgcgcc aaaatctccg caaacccatc
     1441 gtcctgggct tggcggtcat cggcgtgttc gtttttggtc taggcgcctg ggcggccata
     1501 tcgcagatcc ggggcgcggt caccgcgcca ggtgttttcc gggtggaagc cagccggaaa
     1561 acgctcaaaa gccgcgatgg cggcttggtg cgtcagatca atgtgcgtga gggcgatgcg
     1621 gttgcggttg gccagctgct gctgaaattc gacgatactg tccccaaggc gcaggtcgat
     1681 atctacgaga accagtacga tgcggccttg atgcaagccg cccgtctgcg tgcggagatt
     1741 gttcgacgcc ccctgatcgt tcctccagaa ctacaggcgc ggcggactga tccgcgagtg
     1801 tccgagatca tccagaacga aatgacggtc tatgacgtgc gcagggcggc aatcgaatct
     1861 caggccgcaa tcttgaacca gcggttcgag cagcttcaga gcgccagagc cggactgcag
     1921 atccaggccg attccgccgt tgagcagatc gctctctcgg aagaggaact agaaggctac
     1981 aagacgctcc tcgccaaggg ctatgcgcca aaaactctgg ttttacgcct tgagcgccaa
     2041 ttgtcggaaa gcaaggcaag gcgcggctct ctgatggccg agattacacg caacagccaa
     2101 caatccggtg aaacccggct gcagttgtct agcctctatg aacagcgcgc ctctgaatcc
     2161 gccagtaacc tgcgcgacgt cgaggctcgc atcacagatc taggccctcg actgggcgcg
     2221 gctcgagaag ccctggcgca aaccgaaatc cgcgctccgg cggcgggtta cgtcctgggc
     2281 ctcagccaac atacgatcgg cggcgtcgcc gcgccgggcg aggtcctgat ggacgtggtg
     2341 cccagcaatg cgcctctggt gattaccgcc gaggttcgtc ccagcgatat cgacgaggtc
     2401 cagcctggca tgcaggcgca ggtcgcgctg caggcctaca gcagtttccg cgtacccaag
     2461 atccaggctg aggtcctaac tgtctccgcc gacgccctcg ccaacgctga gactaagtct
     2521 tcctattatc gcgtcgattt gcgcatctcg cccgaagagt tgcgaaaact ccccaagggc
     2581 gtgcggcttt ctccgggcat gcaagtttcg gtgatgatca tgacaggtcg gcgcaccatc
     2641 ctgtcttatc ttctgggacc gatcggcgag gtcatcgacc agtccatgcg cgagcaatag
     2701 gccggaatat gaccctggcg gagctcaaac aacccaaggt cgattttctg gttgccggcg
     2761 tgcagaaggg cggcactacg gcgctcttcg actatctgcg cgagcaccct gatttgctgc
     2821 tagcggacgt caaggaagta cattttttcg ataacgagaa tgtcgactgg caaaagcctg
     2881 actatgaatc ttaccatgca cacttccatc cgccggcgga tggaaggcgc aggctgtggg
     2941 gtgaagtaac gccgatctat acctactggc cgcgctgctt tgagcggatt gcggactata
     3001 attcagcgat gaagctcgta ttggtgttcc gaaacccgat tgaaagagcc tggtcgcact
     3061 ggagaatgga atacgctcgt ggggctgaaa cggagccttt cgcatggtgc atacgggagg
     3121 ggcgccatcg cgttaatggt gatccgtcag ctccaggaca tcatcgtgag ttcagctatg
     3181 tcgagcgggg cttttacggc gcccaagttc aacgtct
//
LOCUS       DAOMEO010000259         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3367_length_3215_cov_2.725633, whole genome shotgun sequence.
ACCESSION   DAOMEO010000259 DAOMEO010000000
VERSION     DAOMEO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_3215_cov_2.725633"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLH31_07145"
     CDS             complement(<1..289)
                     /locus_tag="PLH31_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQR89029.1"
                     /translation="MNSPTRLFSQAAASPRRRLLIWLLVATLTSLALAGLTASPLTTR
                     LDNILYDTLQRASSRPVDPSILIVEVDEKSLRDMGAWPWPRTAHARMIDHVT"
     gene            complement(293..1642)
                     /locus_tag="PLH31_07150"
     CDS             complement(293..1642)
                     /locus_tag="PLH31_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQR89030.1"
                     /translation="MTSKRDLTLLATAPLACILGLSCSSAALAAPPVDAPVAYVVKPG
                     DTLIGLASAYLNKPSDYRQVQRANQIARPRALKIGSTLKIDPRLLKSTPIDARLSAVS
                     GAVDLETNGARAPARAGMVLVEGQRLITGPGGFATFEMADESRVTLPSNTSLRIERMR
                     QIILNNAPQRVFQMDRGRGTISATPNPNPSGRFEVRTPVSISAVRGTEFRVGQDPTGA
                     KAQTEVLKGAVGVDLASATTEGPPIPAGFGVSATPAGVSAPVALLPAPKLGEGGQRQS
                     DKIIRFSVEPVPGAATYRLALSRDAGFVEVFAEADSPAPAAEFGEIANGTYFVRLTAL
                     DAGGLEGMPADYSFDRDLDVLEPGAPPSAQVSGKHRNFLFRWSAAGDGVRSYRFQLFS
                     GTDATSPIIDQPGLSEPQLTLTDLPPGTYGWRVSAVRFKGGTFAEKVGAMQTLQIGR"
     gene            complement(1639..2476)
                     /locus_tag="PLH31_07155"
                     /pseudo
     CDS             complement(1639..2476)
                     /locus_tag="PLH31_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518807.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2625..>3215)
                     /locus_tag="PLH31_07160"
     CDS             complement(2625..>3215)
                     /locus_tag="PLH31_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89031.1"
                     /translation="GWPEGRLRDLVVFLGHPFTALILACVGVWLLLGLRYKTPRSTLL
                     KITGRALEPAGAISLVVGAGAAFKQVLIDSGAGAHITAVITAAQVTPLMFAFLVAAIV
                     RIAQGSATVAMVTAAGLAAPMVAAAGLPPAQVALVLIGIGAGATVVSHVNDTGFWLVS
                     QYLGLTESQTFKAWTIGSTLAGLTTLAVAVIASAFL"
BASE COUNT          534 a          977 c         1183 g          521 t
ORIGIN      
        1 ccgtcacgtg gtcgatcatt cgcgcatggg cggtgcgcgg ccagggccag gcgcccatgt
       61 cgcgcaggct tttttcatcg acctcgacga tcaggatcga gggatcgacc ggacgggacg
      121 aggcgcgttg cagggtgtca tacaggatat tgtccagccg cgtggtcagc gggctggcgg
      181 tgagacctgc cagggccaga ctggtcaagg tggccaccag aagccagatc aacaagcggc
      241 gacgcggcga ggctgcagcc tggctgaaca gtcgggtggg cgaattcaag atctagcgac
      301 cgatctgcag cgtctgcatc gcgccgacct tctcggcaaa ggtcccgccc ttgaagcgca
      361 ccgcgctgac ccgccagcca taggttcctg gcggcaggtc ggtgagcgta agttgcggct
      421 ctgagaggcc tggctggtcg atgatcggcg aggtcgcgtc agtccccgaa aatagctgga
      481 accggtagct gcgcacgcca tctcccgccg cgctccagcg gaacaggaag ttgcggtgct
      541 tgcccgaaac ctgggcgctg ggcggcgcgc ccggctccag aacatccagg tcgcgatcaa
      601 agctgtagtc ggcgggcata ccctccaggc cgccggcgtc cagggcggtc aggcggacaa
      661 agtaggtacc gttggcgatc tcgccgaact ccgcggcggg cgcggggctg tcagcctcgg
      721 cgaagacctc gacgaagccg gcgtcgcgcg acagggccag gcgataggtc gccgcgcctg
      781 gcacgggttc gacgctgaag cggatgatct tgtcgctctg gcgctgaccg ccttcgccga
      841 gcttgggggc gggtagcagg gcgactggag cgctgacgcc agcgggcgtc gcgctgacgc
      901 cgaagccggc ggggatcggc ggaccttcgg tcgtggccga ggccagatcg acgccgaccg
      961 cgcctttcag cacctcggtc tgggccttgg cgccggttgg atcctggcca acacggaact
     1021 cggtgccgcg caccgccgag atcgagaccg gggtgcgaac ctcaaagcga ccacttggat
     1081 tgggattggg cgtggcggag atcgttccgc ggccccgatc catctggaag actcgctggg
     1141 gcgcgttgtt caggatgatc tggcgcatgc gctcgatgcg caggctggtg ttggacggca
     1201 gggtgacccg cgactcgtcg gccatctcga aggtggcgaa gcctcccggg ccggtgatca
     1261 gccgctgacc ctcgaccagc accatgccgg cgcgggcggg ggcgcgggcg ccattggtct
     1321 ccaggtcgac ggcgccgctg accgcgctga ggcgggcgtc gatgggcgtg ctcttcagca
     1381 gcctgggatc gatcttcagg gtcgagccga tcttcagggc gcgtggcctg gcgatctggt
     1441 tggcgcgctg cacctgccga taatcgctgg gcttgttcag ataggccgag gccaggccga
     1501 tcagagtgtc gcccggcttg acgacatagg ccaccggcgc atcgacaggc ggggctgcga
     1561 gggcggcgct tgagcaggaa agtcccagga tgcaagcgag gggggcggtg gcgagaaggg
     1621 tgaggtcgcg cttggacgtc atgcttgggt ggctccagct tcggtgtccg cctcgggatg
     1681 gctctgaggg tcacaggctt cgagacgata cccgaaaccg tagaccgtcg ttaggcgaaa
     1741 cccgttggcg gggcgaagct gaagcttgga gcgcaggcgc gagacgtggg cgtccagggt
     1801 gcgggtctcc agatccggcc gctgacccca gacccggcgc aacagatatt cgcgcgacag
     1861 ggggcgggcc agattgttga acaggatcgt cgccagctgg aattccttgg gcgtcagggt
     1921 ctcgacatgg tcgtcccagc tagccgtctg ggcgccgggg tccatgcgat aggggccgtg
     1981 ttgctcaatc tgctgggttg tcgaggggcg gtagacccgg cgggccaggg cgtttacccg
     2041 ggccagcagg accggtggct gcaagggctt gacgatgtaa tcgtcagcgc cggcgttcag
     2101 accctcgacc agatcggcct cgatcgagcg actggtgagc aacagcaccg gcggcggcgt
     2161 ggtcagcatc tcatccagcc ggcgcagggt ctcgatgccg gacaggtcgg gcatgttcca
     2221 gtcgatgatc agcaggtcaa aggtctggcg ccgcatctcg gccagcagac gttcagggtg
     2281 agtgaagctg acgcagtcgt atcctgcggg acccagcaag ccggcgacca gcgcattgtg
     2341 gctctcgtcg tcgtcgagaa gggcgacttt catgcgctca cttagcagag gggaattcgc
     2401 gcgccccgct ctgggcgggg cgcgcggcgg gcgtccgtct gtccggtggg ggggatccag
     2461 atgatcgtcc gcccatgggg aataccttgt tcgcccttgc gtcgcgctag gccaggcaag
     2521 gattttacaa tggtttacag tccggtcgag ccgctttggt cgtcaacggg gtcggcgcga
     2581 gcgccaaaac tcctacgcgc gcgggttgga ttgtcgtttc gtcctcacag gaaggctgaa
     2641 gcgatcaccg caaccgccag ggtggtcagg cccgccaggg tcgatccgat cgtccaggcc
     2701 ttgaaggtct gtgactcggt gaggccgaga tattgactga ccagccagaa gccggtgtcg
     2761 ttgacgtggg agacgacggt ggctcctgcc ccgatcccga tcagcaccag ggctacctgg
     2821 gctggcggca gtccggcggc cgcgaccatg ggggcggcga ggccggccgc cgtcaccatc
     2881 gccacggtgg ccgagccttg ggcgatgcgg acgatggcgg cgacgaggaa ggcgaacatc
     2941 agcggtgtca cctgggcggc ggtgatcacg gcggtgatgt gagccccggc gcctgagtcg
     3001 atcagcacct gtttgaaagc cgcgccggcg cccacgacca ggctgatggc cccagccggt
     3061 tcgagggcgc ggccagtgat cttcagcagg gtggagcgcg gcgtcttgta gcgcaggccc
     3121 agcaaaagcc agacgcccac acacgccagg atgagggcgg tgaaggggtg gcctaagaac
     3181 accaccagat cgcgcagcct gccttcaggc caacc
//
LOCUS       DAOMEO010000260         3211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3374_length_3211_cov_2.476236, whole genome shotgun sequence.
ACCESSION   DAOMEO010000260 DAOMEO010000000
VERSION     DAOMEO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_3211_cov_2.476236"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLH31_07165"
     CDS             <1..397
                     /locus_tag="PLH31_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR89032.1"
                     /translation="TFAVTRKSQADARARQGSSWDQVAAATATLVAYQLSDHGPLLRA
                     SSMGALPTEIRHEMAEGYGRGSERFAAMISDGVADGSIRPVDPMIAAHMINSMLNAAA
                     SLGAWVPGVERDEAAHLFARPLLMGLFTR"
     gene            complement(461..3211)
                     /locus_tag="PLH31_07170"
                     /pseudo
     CDS             complement(461..3211)
                     /locus_tag="PLH31_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518670.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          509 a         1002 c         1149 g          551 t
ORIGIN      
        1 cacctttgcc gtgacgcgca agtcgcaggc cgacgcccgc gcccgccagg gatcgtcctg
       61 ggatcaggtg gccgccgcca ccgccaccct ggtggcctat cagctctccg atcacggccc
      121 gctgctgcgg gcttcgtcca tgggggcctt gcccaccgag atccggcacg agatggccga
      181 gggctatggc cggggctcgg agcggttcgc ggcgatgatt tcggacggcg tcgccgatgg
      241 ctcgatccgc ccggtcgacc cgatgatcgc cgcccacatg atcaattcca tgctcaacgc
      301 cgccgcctcg ctgggggcct gggtgcccgg ggtcgagcgc gacgaggcgg cccatctgtt
      361 tgcccggccg ctgctgatgg gtctcttcac ccgctgagcc aaaacgaaag aagcgcgccc
      421 cgcaaagggc gcgcctccct cttgatcgct ccagcgagac ctagaacttc ttgcgcagac
      481 caatcttgta ggtccggccc agcggatccc cggtgaaggg gtcgtagccg aggtccagac
      541 gggcgtagga cgggtccttg tcaaacaggt tggtgatcgc ggccgtcacc gtggtgtccc
      601 agggcaggaa cacgcgatag gccagatcgg tcagcacctg ggcgtcgatc gtcttgccgt
      661 tggtgatctg cacgcccgcg cccgtggtgt ccttgtaggc gttggccgca aagggcgcgg
      721 tgcgctggtc ggtgtaggag tcgatatagt ggaccgtcag gcgcacattg tgcggcccca
      781 tgctccactc gccatggatg tcgcccttcc actgcgggat tggcacgacg gtggtctggt
      841 agttcagcag gccgacggcg tcgaaggcct tctcgaccac cacgccctcg acgctggtgg
      901 cgccgacctt gtactcggcg atgtaggtgg cgttgccgcc caccgacagc tcgccgccca
      961 gcacgttgtc aaaccggtag tcggccgcga agtcgaagcc ggagttcttc accgccgcgc
     1021 cgttgatgta gttggtcttc aggcgggcga tggtggtggg cggaccgcag acgccgttga
     1081 aggtgaaccg cgccgccagg gccggataaa gggcgcagtt gttggtccca gtggcgccgt
     1141 ttggatagag ggcgttgacg atgcccgcca ccggctcggt gacgatcggg tttttgaagt
     1201 cgaactggaa gtagtcgatg ctggccttga agttgcccgc cttgaacagc aggccggcat
     1261 tgtaggtgtt ggccttttcc ggatcgaggt tcggattgcc gaagatatcg acggcccgga
     1321 aggtgccgag gatgctctgc agcgaggtca ccgaggtggt ggtggtgatc ggatccggcg
     1381 gcccgcggaa ggtggagccg gccgagccgc gaatggccag ccagggcagg gcctgccagc
     1441 gggccgagat cttcggattg aaggtgtcgc ccaccgcgcc gccatagtcc tcgaagcgcg
     1501 cggccagctg cacctggaag ctgtcggtca tcggcaccga cagttcgccg aacgcggcca
     1561 tcacgtcgga ctggttgtcg gcctcgttgc cgacgcccag gaacatgaag gggccgttgc
     1621 gctgcgaccc cgtgcaggtc gtcacgccaa agtcaggcgt gttgacgcag ggattgatcg
     1681 ccgcgttgct gatgtcgttg tagtcggcct tgaaatagct gcggcggtat tgaacgcccg
     1741 ccgcccaggc cacgtcgccg ccggccaggc tgatgccggt cttgccgttc aggacggcct
     1801 caccgacgaa cagccgtgag gactgcttgg tcgacagctt ctggaagaac cacttggcca
     1861 ggtccttgtt gaccgccagg gccgagttgt agcccggatt ggccgcgccg gtgatgggat
     1921 taccggggat cgcgctggcg aaggggttga agtacatgca acccccaacc ccggccgtgc
     1981 cctggatgcc aggcaggctt ggattgctgt cgcatgtcgg gccgcccagg ccttgcagcg
     2041 ccagggcgta gcgattgacc agggtgtcgt agccggtgcg gatgccgacc tcctgggaat
     2101 aggtcagcgc catttcccag ccgatgccgt tgtcgaactc gcccttcagg tcgccggaga
     2161 cgcggaaggc gtcgaaggac cgcgagccga tcgaggggcc gtagccaaag gccgggttgc
     2221 cgccaatgcc gaaggggcgg ttggcgacgt tcagggcgcc gaaggcgaag accgagctcg
     2281 gcacggtgga ctgggcgcca gtgaccaggc tgttcagggt gaccgtgggg ttggcggcga
     2341 tgaacgcctg caggcccggg ttggtcggcg gcacatagta tcgaccggcc agccccgcgc
     2401 tgggcgcgac ctcagccgtg ggcaccgcca gggcggcata ggacggcgag gtgttccact
     2461 cgggcacgtc ggtatgggcg tacagcacct cgacgtggaa cttggtcttg tcagacaggt
     2521 cggcgttgat ctcggcatag acctgcaccc cgtcggactt ttcgacgagg ttgtcgaacg
     2581 gcgtatagtg ccagtagcag accggcgtca gaccgctgaa gccagggtag ccgcccaggc
     2641 cggcgcaggc gggatcgcgc agggcgttgg tggagctcgc cagggggata aagaccgacg
     2701 ggctgcccgc cgccgaccag ccggcttccg gattgttcag atagggcgtg ttggcccagc
     2761 tgcgctcggc gacactgagc ttggagcggt gctgccagcc ggcgctgacc aggaagttgc
     2821 cgttgtccca caccttgccg aacgtggcgt tgaggccgta gtcgccgtcc gagccgtcga
     2881 tgacccggta gtcggcgccc acttccaggc cgctgagccg taggtggcgg ccgcgccgtc
     2941 cttcaggact tccaggcgac cgatggccgc ggcgggaatg atgttggtgt cgacgatgcc
     3001 ggcgccagcc gcgccgaacg ggttgatcgc cagacgacgg ccgttcagca gcaccagcgt
     3061 gcgctgcgag ccgaggccgc gaaggttgac cgagccggag ccttcagacc cctgggcgcg
     3121 gctgtcgaac tggttggtgt cgcccagcac cccgctggag accgacaggg ccttgagaag
     3181 ctcgacggtg gagggggagc cccgcttttg c
//
LOCUS       DAOMEO010000261         3197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3389_length_3197_cov_2.299809, whole genome shotgun sequence.
ACCESSION   DAOMEO010000261 DAOMEO010000000
VERSION     DAOMEO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_3197_cov_2.299809"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2280
                     /locus_tag="PLH31_07175"
     CDS             <1..2280
                     /locus_tag="PLH31_07175"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQR89033.1"
                     /translation="ALGDLAEGLLLTLAAMAAIVQVVFQEARSERALMALRDLAEPMV
                     RLVRDGRERLTSARLLVPGDVFLVGEGQRIPADGRLIDGEVLTIDEAALTGESVPVQR
                     APDPAGGPQAVIFAGTLVVSGQGAAEVVATGPRTAIGKIGVALATLETSAAPLQKAIT
                     RLVRWLGLAAGLFCLLVMVAYGLIRGDWLEAALAGITVAIAMIPEEFPMVLAVFMALG
                     AWRLGRHNVLVRRGAVIETLGGATVLCVDKTGTLTENRMTVAALWADGSRSAPSTPDA
                     SEPHPLLVVASLACAAQPVDPMDRAITALAHPPADARLERVWPLQTERLAVVQAWRLA
                     DQNLLLAAKGAPEAIFALCRLSIAEAAPVRAALEALALEGLRVLAIAEGVRSDVTPDE
                     PEAARFTFRGLVGFADPLRADAADAVREARAAGVKVMMITGDYPATALNIARQAGLDV
                     EAGVLTGEEIAGLDQAALSARLADVRVFARVRPEQKLALVEALKANGEVVAMTGDGVN
                     DAPALEAAHIGIAMGRRGTDVAREAADLILLDDSLASIVGGVRLGRRIYANLRRALTY
                     VTAIHVPIAGLALAPVLLGAPPMLFPVQVVFLELVIDPVCALVFEGEPSARDSMRRPP
                     RSPRASLFGARQIAQGALQGAVILVAVLALYLWALTGMPADQARAGAFVALVVGNLTL
                     AITDATGRDGRLLDSRRWIFWTIAGAAGVILCLVLLTPGLAGLFRMATPSAGVVVLSV
                     ATGLASAGWIWLPRMMRRT"
     gene            2339..>3197
                     /locus_tag="PLH31_07180"
     CDS             2339..>3197
                     /locus_tag="PLH31_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR89034.1"
                     /translation="MAFKDILLQLDSYPEPTPAAVIERGVWWAQRLQASLTALAVDIR
                     IPVHSNRIADYLIGLTDLAHGEEAKAHRQGQDVLALAAACAQRAEVEFLAQTHRAHLY
                     DVGEHVARMARTRDLCIVPLGGRFDEQQGVVRSVVFGSGRPTLILDETAVTARASGCA
                     RVVIAWDGGASAARALADSLQVLAQAQAVTVLTISGDKPGVDNDSGLDVLRHLHRHGV
                     AAEAVTVESGGDPAGSVIDRHVREVDADLLVMGLVHERLIPSGQVAKIENPMLHYSFM
                     NYSQVLQKLD"
BASE COUNT          431 a         1035 c         1163 g          568 t
ORIGIN      
        1 gcgttgggcg atctggccga gggtcttctg ctaaccttgg cggcgatggc ggccatcgtc
       61 caggtggtgt tccaagaggc gcgcagcgag cgggccctca tggctctgcg cgacctggcc
      121 gagccgatgg tgcggctggt gcgcgacggg cgagagcgtc tgacctcggc ccggctgctt
      181 gtccccggcg atgttttcct ggtgggggag gggcagcgca tcccggccga cggccggctc
      241 atcgatggcg aggttctgac gatcgacgaa gcggctctga ccggagaatc cgtcccggtc
      301 cagcgcgcgc cagaccccgc tggtggcccg caggcggtga tcttcgcggg tacgctggtg
      361 gtctccggtc agggggcggc cgaggtggtc gccacggggc cacggaccgc catcggcaag
      421 atcggcgtgg cgctggccac gctcgagacc tcagcggcgc cattacagaa agccatcacg
      481 cggctggtca ggtggctcgg tctcgccgcc ggcctgtttt gcctgctggt catggtggcc
      541 tacgggctga tccggggcga ctggctcgaa gcggctctgg cgggcatcac cgtcgccata
      601 gccatgatcc ccgaagagtt tccgatggtg ctggcggtct tcatggccct gggcgcatgg
      661 cggcttggcc ggcacaatgt gctggtgcgc cgtggcgcgg tgatcgagac cctcggcgga
      721 gccacggtgc tctgcgtcga caagaccgga accctgaccg agaaccgcat gacggtggcc
      781 gcgctctggg cggacgggtc ccggagcgcg ccctcgacgc cggacgcctc cgagccgcac
      841 cctttgctgg ttgtcgcgag tctggcctgc gccgcgcagc ccgttgatcc gatggatcgg
      901 gcgatcaccg ccctggccca cccgcccgcc gacgcccgcc ttgagcgggt ttggcccttg
      961 cagaccgagc gtctcgcggt ggtccaggcg tggcggttgg ccgaccagaa cctgctgctg
     1021 gccgccaagg gcgcgcccga ggcgatcttc gccctttgcc gtctttctat cgctgaggca
     1081 gcgccagttc gggcggcgct tgaagccctg gcgcttgaag gcctgcgcgt ccttgccatc
     1141 gcggaggggg tccgcagcga tgtgacgccg gacgagcccg aggccgcgcg gttcaccttt
     1201 cggggtctgg tgggttttgc cgatccgctg cgggctgacg cggccgacgc tgtgcgcgag
     1261 gcgcgggcgg ccggtgtcaa ggtcatgatg atcaccggcg actatcccgc cacggccctg
     1321 aacatcgcgc gccaggcagg ccttgatgtc gaggcgggcg tcctgaccgg tgaggagatc
     1381 gccggcctgg atcaggcggc gctgagcgcg cgtctggcgg acgtgcgggt ctttgcgcgc
     1441 gtgcggcccg aacagaagct ggcgctggtg gaagccctga aggccaatgg cgaagtcgtc
     1501 gccatgaccg gcgacggcgt caacgatgcg ccggccctgg aggcggctca cattggcatc
     1561 gccatgggcc ggcgtgggac agacgtggcc cgcgaagccg ccgatctgat ccttctggac
     1621 gacagtctgg cctcgatcgt gggcggcgtc cgtctgggtc ggcggatcta tgctaacctg
     1681 cggcgcgccc tgacctatgt caccgccatc cacgtgccta tcgcgggtct cgcgctggcg
     1741 ccggtgctgc tgggcgcgcc gccgatgctg tttccggttc aggtggtgtt ccttgagctg
     1801 gtcatcgatc cggtctgcgc cctggtgttc gaaggcgagc cgagcgcgcg cgacagcatg
     1861 cggcgaccgc cgcgttcgcc ccgggccagc ctgtttggcg cgcggcagat cgcgcagggg
     1921 gcccttcagg gggcggtcat cctggtcgcc gtgctggcgc tctatctctg ggccctgacc
     1981 ggaatgcccg ctgaccaggc gcgggccggc gccttcgtgg cgctggtggt cggcaatctg
     2041 acgctggcca tcactgacgc caccgggcgt gacggacggc tgctcgactc gcgacgctgg
     2101 attttctgga ccatcgccgg cgccgccgga gtgatcctgt gtctggttct gttgacgccg
     2161 ggcctcgcgg gtctctttcg gatggcgacg ccatcggcgg gggttgttgt gctcagcgtc
     2221 gcgacgggcc tggcctcagc cggctggatc tggctgccca ggatgatgcg gcggacctga
     2281 tccagatcaa ggccggaggt ccaacgaggg ctgaaatggt atcgaaagga gccttccgat
     2341 ggctttcaag gacattctcc tccagctgga cagctatccg gaaccgaccc cggcggctgt
     2401 gatcgagcgc ggcgtttggt gggcgcagcg cctgcaggcg tcgcttacgg ccctggccgt
     2461 cgatatccgg atccccgtgc acagcaacag gattgccgac tatctgatcg gcttgaccga
     2521 tctggcgcac ggcgaagaag ccaaggccca tcgccagggt caggatgtgc tggccctggc
     2581 ggccgcctgc gcccagcgcg ccgaggtcga gttcctggcc cagacccatc gcgctcatct
     2641 ctatgatgtc ggcgagcacg tcgcccggat ggcccgcacc cgagacctgt gtatcgtgcc
     2701 gctcgggggg cggttcgacg aacagcaggg cgtggtcagg agcgtggtgt ttggttctgg
     2761 tcggccgacc ctgattctgg acgagaccgc tgtgacggcg cgcgccagcg gctgcgcacg
     2821 ggtggtgatc gcctgggacg gcggcgccag cgcggctcgc gccctggccg acagtctgca
     2881 ggtcctggcc caggcccagg cggtgacggt gctgaccatc agcggcgaca agcccggggt
     2941 tgataatgac agcggcctgg atgtcctgcg ccaccttcac cgccatggcg tcgccgctga
     3001 ggccgtgacc gtggagtcgg gcggggaccc ggccgggtcg gtcatcgatc gccatgtccg
     3061 tgaggtcgac gccgacctgc tggtgatggg cctcgttcat gagcgactca ttccgagtgg
     3121 gcaagtagca aagattgaga atcccatgtt gcactacagt ttcatgaact attcacaagt
     3181 gcttcaaaag ttagatc
//
LOCUS       DAOMEO010000262         3194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3395_length_3194_cov_2.919720, whole genome shotgun sequence.
ACCESSION   DAOMEO010000262 DAOMEO010000000
VERSION     DAOMEO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_3194_cov_2.919720"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLH31_07185"
     CDS             <1..699
                     /locus_tag="PLH31_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQR89035.1"
                     /translation="IGARKPDEKRTFGYRRFEILAAAFNAILLFAVAIYVLVEGVKRI
                     IDPEPVQSTGMLIVAILGLVINLIAMRLLSAGKDKSLNIKGAYLEVWADMLGSLGVIA
                     GALVIQFTGWRFVDPIVAIGIGLWVLPRTWILLKDTTHVLLEGAPSGVALAEVRKIVS
                     ETPGVANVHDLHIWVSGADQPSLSVHVVLEPGADGETVRLAIAARLEGDFDLHHATIQ
                     TEVVACGAQEALHS"
     gene            696..1163
                     /locus_tag="PLH31_07190"
     CDS             696..1163
                     /locus_tag="PLH31_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQR89036.1"
                     /translation="MTQTSKRQGLVLTSTQAVAARPEAVFKLLDDYPGYGAWAPFLTI
                     SPTAKETDPNRMDFAIRLDQLKKPVVLFGKVTAREPHRLVAWSLGARGALTLRESFTI
                     ERAMAGSVIIHHVTFEGVLRWVLGPPLLRLYRPVFGTLDRALRRRLGGPKARR"
     assembly_gap    1673..1682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1683..3167)
                     /locus_tag="PLH31_07195"
     CDS             complement(<1683..3167)
                     /locus_tag="PLH31_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89037.1"
                     /translation="MANLPLLPGRAALRVVVYRDSQSGYIANPALRLRRVNSSKRDGL
                     RASLRASISTHWSATLGLTHQSINNDDTQYGFRRLGSHLRDNPVREPHDNDFDHVSLS
                     LTGDGAWGRVSGSVSRLSHHFDSRYDATSASALYGLAGASAALDEAKAIDLSVAEVTY
                     ATPADRRLHGLVGAFASTGDIRLSSALHPVMGSATNAYAERRRDKINETAVYGELTWD
                     LSRRLSATAGLRWFDFRFETDSTVSQAIGQRQFSSAADATGLSPKALVSYDARPGLLL
                     YAQAAEGYRPGGFNTAGRVGQVFDAPGAPARRYSADELWNYEVGAKLRAWNDRLQGRV
                     AVFYATWESVQSDQYLTDGTAFTANVGNGDNRGIEAEAAIRLTDQIDLRAAVLLNDPQ
                     ITRQNPDFSSRSDAGLPGVSRASASFGVDYHHDLAGDRTIRLQSQATYVGASYLTFDA
                     DRRHEMGEYLGLRGVVSLEAARWSLSATLDNPLGANGNSFSFGNP"
BASE COUNT          550 a         1020 c         1045 g          569 t
ORIGIN      
        1 atcggggcgc gcaaacccga cgaaaagcgc accttcggct atcgccgctt cgaaatcctc
       61 gccgcagcct tcaacgcgat cctgctgttc gcagtcgcca tctacgtcct ggtcgagggc
      121 gtcaaacgca tcatcgaccc cgagccggtg cagtcgaccg gcatgctgat cgtcgcgatc
      181 ctgggccttg tcatcaattt gatcgccatg cgcctgctta gcgccggcaa ggacaagagc
      241 ctgaatatca agggcgctta tctggaggtc tgggccgaca tgctcggctc actgggcgtc
      301 atcgccgggg ccctggtcat ccagtttacc ggctggcggt ttgtcgatcc gatcgtggcc
      361 ataggcatcg ggctgtgggt tttgccgcgc acttggatcc tgctgaagga caccacccac
      421 gtcctgctgg aaggcgcgcc cagcggcgtc gccctcgccg aggtccgcaa gatcgtctcc
      481 gaaacgccag gcgtcgccaa tgtccacgat ctgcacatct gggtctcggg cgcggatcaa
      541 cccagtcttt ctgtccatgt ggtgctggag ccaggggccg acggcgagac cgtccgcttg
      601 gccatcgcgg cgcggctgga gggcgacttc gacctgcacc acgctaccat ccagaccgaa
      661 gtcgttgctt gtggggccca ggaggcgctc cactcatgac ccagacctcg aaacgtcaag
      721 gtctggtcct gacctccacc caggctgtgg ccgcgcggcc tgaggcggtg ttcaagctgc
      781 tcgacgacta tccgggttat ggggcctggg cgccattcct aaccatctcg cccactgcca
      841 aggagaccga tcccaaccgc atggatttcg cgatccgcct tgatcagctc aagaagcctg
      901 tggtcctgtt tggcaaggtg acggcccgcg agccgcatcg tttggtcgcc tggtcgctgg
      961 gcgcgcgcgg cgccttgacc ctgcgcgaga gcttcacgat tgagcgcgcc atggccgggt
     1021 ccgtgatcat ccatcacgtc accttcgagg gtgtcctgcg ctgggtgctc ggtccgccac
     1081 tgttgcggct ctaccgaccg gtgttcggca ccctggaccg cgcgctgcgc cgccggctcg
     1141 gcggcccgaa agcccggcgg tgaagatcgc ccctggagac gcctggccgg ggcgggacga
     1201 agcgcttgga ctggtgacca tcaagaccgg ttccatcgtc acccaagacg gccatcgcca
     1261 ccgcgctagc ccttgagcct ggagatcacc tcatggccat cgtcacgtcg cacgccgaat
     1321 ggcgatacgc ccggtgggca tgctcaccat catggcggaa tcttcggcga acgcaccgag
     1381 ctgatcttcg cggtcggttc gggcgtcacc ttggtcgccg gttggctgat tgaccagcgc
     1441 gtcgcgagac tcgcgtccct gatctgctac tgcatcgctt ccagcctcgg ccgacgcagg
     1501 gctcatcccg aacatacggc agggaccacc ttggcggtcg gccgcctcgt cgagcctgga
     1561 aaccggcggc tcgttccgcc gcccgttgtt agaaagccaa gacctagaag cgtgcagtca
     1621 gccgcagggc cacgctcctt gggcgtggcg gggtgataat ctgctcgcgg gtnnnnnnnn
     1681 nncggattgc caaacgaaaa gctgttgccg ttggccccta aaggattgtc gagggtggcg
     1741 ctcaagctcc agcgcgcggc ttcgaggctg acgacgccgc gcaggcctag atactcgccc
     1801 atctcgtggc gccggtcggc gtcgaaggtc aggtaggacg cgccaacata ggtcgcctgg
     1861 ctctgcagac ggatggtgcg atcgcctgcc aggtcgtgat ggtaatcgac gccgaagctg
     1921 gccgaggcgc gagagacgcc cggcaggcca gcgtcgctgc gcgaactgaa atcaggattt
     1981 tgccgcgtga tctgcggatc gttaagcaaa acggccgccc gcagatcgat ttgatcggtc
     2041 aggcggatgg ccgcctcggc ctcgataccg cgattgtcac cgttgccgac gttcgcggta
     2101 aaggctgtac catcagtcag atactggtcg ctctggacgg actcccaggt cgcatagaac
     2161 acagccacgc ggccttgtag tcgatcgttc caggcgcgca gcttggcgcc gacctcatag
     2221 ttccaaagtt cgtcggccga gtagcggcgc gcgggagcac cgggcgcatc gaagacttgg
     2281 ccaacgcggc ccgcagtgtt gaagcctcct gggcggtaac cttcagcggc ttgagcgtag
     2341 agcagcagac ccggccgggc gtcgtagctg acgagagcct ttggcgacag gccggtggcg
     2401 tcggccgcgc tgctgaattg tcgttgacca atggcctggc tcacggtgga atcggtctca
     2461 aagcggaagt cgaaccagcg caggccagcc gtggccgaca ggcggcggct gaggtcccag
     2521 gtcagttcgc catagaccgc agtctcgttg atcttgtcgc ggcggcgttc ggcataggcg
     2581 ttcgtcgcgc tccccatgac cgggtgaagc gcgctactca ggcgaatgtc gccggtggag
     2641 gcgaaggcgc cgaccagccc gtgcaagcgg cggtcggcag gcgtagcgta ggtaacttcg
     2701 gcaacggaga gatcaatggc cttggcctcg tcaagagctg cggaggcacc ggccaagccg
     2761 tagagcgcag aggcgctggt agcgtcgtaa cggctgtcaa aatggtgcga aaggcgcgag
     2821 acggaaccgc tcacccgccc ccaggcacca tcccctgtca ggctcaggga gacgtgatcg
     2881 aagtcattgt catgcggctc gcgaacagga ttgtcacgta gatgcgagcc caggcgtcga
     2941 aagccatact gagtgtcgtc gttgttgatc gactgatggg ttaggccaag ggtcgccgac
     3001 caatgggtgg aaatcgacgc gcgcagactc gcccgcaggc catcgcgctt tgagctgttg
     3061 acccgcctga gccgaagggc tggattggct atgtagccgc tctgagagtc gcggtagacc
     3121 acgacccgca gggcggcgcg acccggtagc agcgggaggt tggccatcgc ctctagctcg
     3181 tcgccacgac cgcc
//
LOCUS       DAOMEO010000263         3191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3397_length_3191_cov_2.377551, whole genome shotgun sequence.
ACCESSION   DAOMEO010000263 DAOMEO010000000
VERSION     DAOMEO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3397_length_3191_cov_2.377551"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLH31_07200"
     CDS             <1..646
                     /locus_tag="PLH31_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89038.1"
                     /translation="VLEDAFLMPTTMRQLAVVQFFSWFGLFAMWIYTTPAVSAWHFQA
                     IDTTSKAYNEGADWVGVLFAVYNGVAALAALLIPTMVRLTNRRISHAACLALGAAGLL
                     SIPLIRDPGLLWISMIGVGFAWSSILSAPYSILAGALPARKMGVYMGIFNFFIVIPQL
                     LAATVLGLLLKTFFGGQAIYALVLGAAAFLAAAVSVFAVTDPDEVPKQSSAQRA"
     gene            675..>2197
                     /locus_tag="PLH31_07205"
     CDS             675..>2197
                     /locus_tag="PLH31_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HQR89039.1"
                     /translation="MRMLKTLALTTTAACALAGAAAAQTAPGAPGAPSTWAYAAKTGV
                     GASYEAYTDGAYKDGGRTGTISKVWFSIADGVLTETMYGLIHEAQIKQMRFAVQTSTG
                     LAIEGADTVAKTEYLHVDAEGRPLSPAYRITTTDKQGRFVIEKAIFTDPDRQSLFLRV
                     TVKALKGSVTPYLLLEPHMANTGANDAGEASNMALTANEGETFLTLKGSKPFAAASAG
                     FIGVSDGLTDLKDGKLDALYASTGADRGAIMLTGQLPATRGEVTVDYAIGFGPTAAAS
                     AQAADGSLKTGYDEVLAGYNSGWESYLASLVELPRLREASMDGGKLLQASALMLKVQE
                     DRTHSGALIASLSNPWGDTVDASKPSTGYKAVWPRDFYQCAMALAALGDIQTPLAAFH
                     YLPTVQVGPNTPGNTGAGGWFLQKAEVDGTPEWVGVQLDQTAMPIMLGWKLWKQGWLS
                     DDALKTFYGKMIKPAADFLVDGGKVGLGWNSATIVPPFTQQERWEEQGGHSPSTTAAV
                     "
     assembly_gap    2198..2207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2208..2934
                     /locus_tag="PLH31_07210"
     CDS             <2208..2934
                     /locus_tag="PLH31_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HQR89040.1"
                     /translation="ESRMVTTKGSLGDGQYFLRLNSDQDPDNKSRVEVRNGQQPVAED
                     QMLDAGFLELVRYGVRPADDPAVLASLPEIDDQSLEDLYRIRYAFTFPGVEGSFPGWR
                     RYGVDGYGEDTKTGANYGAGDQMRPGQRGRVWPIFTGERGHYEIARITRRGKPAPGAL
                     EPVRQTYVKAMELFANDGLLIPEQVWDGVGADTAHPYVRGEGTDSATPLAWSHAEYVK
                     LLRSVADGVVWDSYQPVKARYAR"
     assembly_gap    2938..2947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          513 a         1109 c         1044 g          505 t
ORIGIN      
        1 ggtgctggaa gacgccttcc tgatgcccac caccatgcgc cagctggcgg tggtgcagtt
       61 cttctcgtgg tttggcctgt tcgccatgtg gatctacacc acaccggccg tatcggcctg
      121 gcacttccag gcgatcgaca cgacctcaaa ggcctataat gaaggcgccg actgggtggg
      181 cgtgctgttc gccgtctata acggtgtggc ggccctggcg gccctgctga tcccgaccat
      241 ggtgcggctg accaatcgcc gcatcagcca cgcggcctgt ctggcactgg gggctgcggg
      301 cctgctatcg atcccgctga tcagggaccc tggcctgctg tggatttcga tgatcggcgt
      361 cggctttgcc tggagctcga tcctgtcggc tccgtattcg atcctggccg gagctctgcc
      421 ggcccggaag atgggcgttt atatgggcat ctttaatttc ttcatcgtca tcccgcagct
      481 gctggccgcc accgtgctgg gcctgctgct gaagaccttt ttcggcggcc aggcgatcta
      541 cgccctggtg ctgggcgcgg ccgccttcct ggccgccgcc gtctcggtct tcgccgtcac
      601 cgaccctgat gaggtcccca agcagtcaag cgcgcaacgc gcctgaccgc tcctccagtc
      661 acgaggcttt ccccatgcgt atgctcaaaa ccctggccct gacgacgacc gccgcctgcg
      721 cgctcgccgg cgccgccgcc gcccagaccg cccccggcgc accgggcgcg ccctcgacct
      781 gggcctatgc cgccaagacc ggcgtcggcg catcctacga ggcctacacc gacggggcct
      841 acaaggacgg cggacggacg gggacaatct ccaaggtctg gttctcgatc gccgacggcg
      901 tgctgaccga gaccatgtac ggcctgatcc acgaggccca gatcaagcag atgcggtttg
      961 cggtgcagac ctcgaccggc ctcgccatcg aaggcgccga caccgtggcc aagaccgaat
     1021 acctccacgt cgacgccgag ggccggccgc tgtcgccggc ctacaggatc accacgactg
     1081 acaagcaggg ccgcttcgtc atcgagaagg cgatcttcac cgaccccgac cgccagagcc
     1141 tgttcctgcg cgtcacggtc aaggcgctga agggttcggt gacgccgtac ctgctgcttg
     1201 aaccgcacat ggccaacacc ggcgccaatg acgcgggcga ggccagcaat atggccctga
     1261 ccgccaatga gggcgagacc ttcctgaccc tgaagggctc aaagcccttc gccgccgcca
     1321 gcgcaggctt catcggcgtg tccgatggcc tgaccgacct caaggacggc aagctggatg
     1381 cgctgtacgc cagcaccggc gcggatcgcg gcgcgatcat gctgacgggc cagctgcccg
     1441 cgacccgcgg cgaagtcacc gtcgactacg ctatcggctt tggccccacc gccgccgcca
     1501 gcgcccaggc cgccgacggc tcgctgaaga ccggctatga cgaggttctg gccggctaca
     1561 acagcggctg ggaaagctat ctggcctcgc tggtcgagct gccgcgcctg cgtgaggcct
     1621 cgatggacgg cggcaagctg ctccaggcca gcgccctgat gctgaaggtg caggaagacc
     1681 gcacccattc cggcgccctg atcgcctcgc tgtccaatcc gtggggcgat acggtggacg
     1741 cctccaagcc ctcgacgggc tacaaggccg tctggccgcg cgacttctac cagtgcgcca
     1801 tggctctcgc ggccctgggc gacatccaga ccccgctggc cgccttccac tatctgccga
     1861 ccgtccaggt ggggcccaac accccgggca acaccggcgc cggcggctgg ttcctgcaga
     1921 aggctgaagt cgacggcacg cccgaatggg tcggcgttca gctggaccag acggccatgc
     1981 cgatcatgct gggctggaag ctgtggaagc agggctggct gtccgatgac gcgctgaaga
     2041 ccttctacgg caagatgatc aagccggccg ccgacttcct ggtcgatggc ggcaaggtcg
     2101 gcctgggctg gaacagcgcc accatcgtcc cgccgttcac ccagcaggag cgctgggaag
     2161 agcagggcgg ccattcgccc tcgaccacgg cggcggtnnn nnnnnnncga gagccggatg
     2221 gtcaccacca agggctcgct gggcgatggc cagtacttcc tgcgcctcaa cagcgaccag
     2281 gaccccgaca acaagagccg cgtcgaggtg cgcaacggcc agcagccggt ggccgaggac
     2341 cagatgctgg acgccggctt cctggagctg gtccgctacg gcgtgcgccc ggccgatgat
     2401 ccggcggtgc tggccagcct gcccgagatc gacgatcagt cgctcgaaga cctgtaccgc
     2461 atccgctacg ccttcacctt cccgggcgtg gagggcagct tccccggctg gcgccgctat
     2521 ggcgtcgacg gctatggcga agacaccaaa accggcgcca actatggcgc gggcgaccag
     2581 atgcggccgg gtcagcgcgg ccgggtctgg ccgatcttca ctggcgagcg cggccactac
     2641 gagatcgccc gcatcacccg gcggggcaag ccggcgcccg gcgcgctgga gccggtgcgc
     2701 cagacctatg tcaaggcgat ggagctgttt gccaatgacg gcctgctgat ccccgaacag
     2761 gtctgggacg gcgtcggggc cgacaccgcc catccctatg tgcgcgggga gggcacggac
     2821 tcggccaccc ccctggcctg gtcgcacgcc gagtacgtca agctgctgcg ctcggtcgcc
     2881 gacggcgtgg tctgggacag ctatcagccc gtcaaggcgc ggtacgcgcg ctaggctnnn
     2941 nnnnnnnggg gggggtggcc ggggggccgg ggggggggca agctcggcca atatccagac
     3001 agccccttca gtcgctccac gacagctccc ccagcggggg agccgcggcc aaagtgtcgc
     3061 acgcgggcgt ggttaacgct ggattaggat tcaatcgcga tgactaggca ggcctgatcg
     3121 cccctaggag tctcttgcca tgtccgcgtt ccgtcgtttg acgatcgcct ggaaactgat
     3181 cctggtcgcg g
//
LOCUS       DAOMEO010000264         3189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3398_length_3189_cov_2.397256, whole genome shotgun sequence.
ACCESSION   DAOMEO010000264 DAOMEO010000000
VERSION     DAOMEO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_3189_cov_2.397256"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..454
                     /locus_tag="PLH31_07215"
     CDS             35..454
                     /locus_tag="PLH31_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQR89041.1"
                     /translation="MQVKNAVYPPGHQAMAFFGAPEDGPFVMVNLLKFKDKAEYPDGS
                     PSDLTGRQAYALYGAEVSKLVEGLGGRIRFSGHVTSILLGEVEELWDAVALAEYPSLA
                     AFQAMAMSPEMHAIEHHRMAGLAGQLNIRTKEGFQAP"
     gene            complement(537..2204)
                     /locus_tag="PLH31_07220"
     CDS             complement(537..2204)
                     /locus_tag="PLH31_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR89042.1"
                     /translation="MRILKWTGVAFAVLTGLLAGAWLGRDFLIYQIGRLMVPKGTENK
                     PVAWRQGPDQATMPADQRPPNVIFILADDLGFNDVSLNGGGVAGVVKTPHIDSIAQQG
                     VDLTNGYSGNATCAPSRAALMTGRYPTRFGFEYTPTPVGFSRLVGTFRTPGALHPPHF
                     NRDEAKDMPPIDELAVPASEVTIAEVLKTRGYHTVHLGKWHLGGAKGSRPEEQGFDES
                     LGFTQGAAMFLPENDPGVVNSKQDFDPIDKFLWANLPYAVQYNGQPMMKARGFMTDYL
                     SDEAIKSIKANKNRPFFMYLAYNAPHTPLQATKADYDALPQIKDHRLRVYGAMIRNLD
                     NNIGRVLQALKDQGLSDNTLVVFTSDNGGAHYVGLPQINSPYRGWKATFFEGGVRVPY
                     YMKWPKGLPAGAKFSSPVGHIDMFATAAGAAGAPMPTDRPIDGVNLIPHLTGKNPARP
                     HEALFWRSGDYRMVRAGDWKLQVLTMPDGQPEKLWLFDLAADPTEQKNLAAANLAKVA
                     ELKALLANRDKGNLKPTWPSLLRGAIAIDHPLSVKDKPTDEFIYWNN"
     assembly_gap    2419..2428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2429..2741
                     /locus_tag="PLH31_07225"
     CDS             <2429..2741
                     /locus_tag="PLH31_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89043.1"
                     /translation="AGLDLTSNRQAILSFMATLGLAQIAKGLFELYVVARRRDLVTLF
                     LSMQALDTLLAVANLYFWRPLPVSVPGQPFNLVLLALQLVALMLAVRAAPSSPAGPAA
                     T"
     gene            complement(2698..>3189)
                     /locus_tag="PLH31_07230"
     CDS             complement(2698..>3189)
                     /locus_tag="PLH31_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR89044.1"
                     /translation="PLWFSYFMQDIHREAAFLGLPFRWPIPDPVKMDFATRTYPKEQP
                     HIHRLTHMGVAAAELGRGLPYLTQVSRVIWSGEVDDWHEGDHLAHAAERAGLDPAQLT
                     RMADTETERLAAIVEESQVAQRAAGHYGVPMIAFNGEAFFGQDRFDQFKWRLDQQGLT
                     ARP"
BASE COUNT          504 a          989 c         1060 g          626 t
ORIGIN      
        1 ctggtccgtc gcgcccacgc ctgaggagag agccatgcag gtcaagaacg ccgtctatcc
       61 gcccggtcac caggccatgg ccttttttgg cgcgccggag gatggcccct tcgtgatggt
      121 caatctgctg aagttcaagg acaaggccga atatccggac ggctcgccgt cggacctgac
      181 cggccggcag gcctatgcgc tttatggggc cgaggtcagc aagctggtcg aggggctggg
      241 cggtcgcatc cgctttagcg gtcacgtcac cagcatcctg ctgggcgagg tcgaagagct
      301 ttgggacgcc gtggccctgg ctgaataccc ctcgctggcg gccttccagg ccatggccat
      361 gtcgcccgag atgcacgcca tcgagcatca ccgcatggcg ggtctggcgg gccagttgaa
      421 catccggacc aaagaggggt tccaggcgcc ctgaagggca gcccaacgtc tctgaacagg
      481 ggaaagcccc gcccttcatc gaagggcggg gcttttgttt gctctccagc gatcgtctag
      541 ttgttccagt agatgaactc gtccgtcggc ttgtccttga cgctcagcgg gtgatcgatg
      601 gcgatggcgc cgcgcagcag cgagggccag gtgggcttga ggttgccctt gtcgcggttg
      661 gccagcaggg ctttcagctc cgccaccttg gccagattgg cggcggccag gttcttctgc
      721 tccgtcggat cggccgccag gtcgaacagc cacagctttt ccggctggcc atcgggcatg
      781 gtcagcacct gcagcttcca gtcgccggcc cgcaccatgc gatagtcgcc cgagcgccag
      841 aacagcgcct catgcggtcg ggcggggttc ttgccggtca ggtgggggat caggttgacg
      901 ccgtcgatgg gccgatccgt cggcatgggc gcgccggcgg ctccggcggc ggtggcgaac
      961 atgtcgatgt ggcccacggg gcttgagaac ttcgcccccg ccggcaggcc cttgggccat
     1021 ttcatgtagt agggcacgcg cacgccgccc tcgaagaaag tggccttcca gccgcgatag
     1081 gggctgttga tctggggcag gcccacatag tgcgccccgc cgttgtcgct ggtgaagacc
     1141 accagggtgt tgtcggagag accctgatcc ttcagcgcct gcagcacccg gccgatattg
     1201 ttgtccaggt tgcggatcat ggcgccatag acccgcaggc gatgatcctt gatctgcggc
     1261 agggcgtcgt aatcggcctt ggtcgcctgc agcggcgtgt gcggggcgtt ataggccaga
     1321 tacatgaaga acggtcggtt cttgttggcc ttgatgctct tgatggcctc gtccgacaga
     1381 tagtcggtca tgaagcccct ggccttcatc atcggctggc cattgtactg tacggcatag
     1441 ggtaggttgg cccacaggaa cttgtcgatc gggtcgaagt cctgtttgga attgaccacg
     1501 ccggggtcgt tttccggcag gaacatcgcc gcgccctggg tgaagcccag gctctcgtca
     1561 aagccctgct cttcgggccg tgagcccttc gcgccgccca ggtgccactt gcccagatgg
     1621 acggtgtgat agccgcgggt cttcagcacc tcggcgatgg tgacttccga agccggcacc
     1681 gccagctcgt cgatcggcgg catgtccttg gcctcgtcgc ggttgaagtg cggcgggtgc
     1741 agggcgccgg gcgtgcggaa ggtgccgacc aggcgcgaga agccgaccgg ggtcggggta
     1801 tactcaaagc cgaagcgcgt gggatagcgg ccggtcatca gcgccgcgcg cgagggcgcg
     1861 caggtggcgt tgccggaata gccgttggtc aggtccacgc cctgctgggc gatggagtcg
     1921 atatggggcg tcttgaccac gcccgcgaca ccgccgccgt tcaggctgac atcgttaaag
     1981 cctagatcgt cggccaggat gaagatcaca ttgggcggcc gctggtcggc gggcatcgtg
     2041 gcctggtctg gtccctgtcg ccaggcgact ggcttgttct cagtcccctt ggggaccatc
     2101 aggcgaccga tctgatagat caggaagtca cggccaagcc atgcgcctgc caataatccc
     2161 gttagcacgg cgaacgccac gcctgtccac ttcagaattc gcatccaggg gctcccttga
     2221 gatcgactga cttttcgctg tcgttgcgga tcactatata ttgacactat aaatgccgaa
     2281 agatgtgatc gtctttcatt cccaatcttt ggctagggtc gacccgatgc gatggcttcc
     2341 gacattggtt ctcgccctga tgggcctgat aaatctggga cgcggcgcga tacacgcctt
     2401 cgccgccgac ggcggggcnn nnnnnnnncg ctggccttga tctgaccagc aaccgtcaag
     2461 ccatcctgtc cttcatggcg acgctcggcc tggcccagat cgccaaaggg ctgttcgaac
     2521 tctatgttgt ggcgcgtcgg cgcgacctcg tgaccctgtt cctgtcgatg caggccctcg
     2581 acaccctgct ggcggtcgcc aatttgtatt tctggcggcc cttgccagtg agcgtgccgg
     2641 gacaaccttt caatctggtc ttgctggcgc ttcagctggt tgccttgatg ctggcggtca
     2701 gggccgcgcc gtcaagccct gctggtccag ccgccacttg aactggtcga agcggtcctg
     2761 gccgaagaag gcctcgccgt tgaaggcgat catcggcacg ccgtagtggc ccgctgcgcg
     2821 ctgggcgacc tggctttcct cgacgatggc ggccaggcgc tcggtttccg tgtcggccat
     2881 ccgggtcagt tgggcgggat ccaggcccgc ccgttcagcg gcgtgggcca ggtggtcacc
     2941 ctcatgccag tcatcgacct cgccgctcca gatcacgcgg ctgacctgcg tcaggtaggg
     3001 caggccgcgc cccaactcgg cggccgccac gcccatatgg gtcaggcgat gaatgtgcgg
     3061 ctgctccttg ggataggtgc gggtggcgaa gtccatcttt accggatcgg ggatcggcca
     3121 gcggaagggc aggcccagaa acgccgcctc gcggtggatg tcctgcatga aatagctgaa
     3181 ccacagcgg
//
LOCUS       DAOMEO010000265         3182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3407_length_3182_cov_2.514231, whole genome shotgun sequence.
ACCESSION   DAOMEO010000265 DAOMEO010000000
VERSION     DAOMEO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_3182_cov_2.514231"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLH31_07235"
     CDS             complement(<1..301)
                     /locus_tag="PLH31_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQR89045.1"
                     /translation="MSPEKQSIRSFTRSIGGSSLPVLVGPATILGLAAMGAVQPAPAW
                     LAAIASGLGGLYLAARHRRIAIENLPAAAQAPLAARDLPPFATILERLPDPLMVVA"
     gene            480..>3182
                     /locus_tag="PLH31_07240"
     CDS             480..>3182
                     /locus_tag="PLH31_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQR89046.1"
                     /translation="MQRKVLVATVAAAPLLALAFGAYAETSVTTARTTPIATTTANNG
                     AADDVRVTADGSIKPTTSGALITLDSNHKVTNLGTLATVGVSDSTGILILGGRTGSVS
                     NGASITINEDYTPTDTDSDGDTDGAFAQGSNRYGVRLTGPGAFTGNIVNETAGAIAIE
                     GNNSAGISLESALVGNFTNNGSVTLLGDNGAGIRIAAPVTGKVTVNGGVSVVGKDTVG
                     VAVDADVSGAFVVQGGVSATGYRFTTRPSILADRNKLDADDLLQGGSALRVTANVAGG
                     VLLDIPPADTDTTKTDDDGDGVADASEGSASLAAYGKAPALLIGSDTRAVTVGAVGTG
                     DDAYGLVARGAVSASGVYDGVAGTAIQIGGNAGQAATLAGGAKLSSTVAASAYEANAT
                     GVQLTAGASVPTLWNQGSMSVFTVSEGAFDSRAVAIDAGASMTTFRNDGSITSTVGGE
                     KGDAYGVIDYSGLLTTITNNGKISTSVVATDDAEDKDDADTLPGNETVTGKSVAIDVS
                     RNTTGVTLVQAGLSDGDDAADGVADPDADGDGVDDSDEPSMLGAVRFGSGADTFNVLN
                     GSVIGDISFGAGADTFRIDNGSLVLGALTDSDGQLAITIGEGSLGLSNAAVINATSLD
                     VGSAGKILFTADPTAGTNTRLQVSGAANIASGAELGIRLTGLVKQPTSYTVISAGSLT
                     VGSIGQGLLSDSPYLYVASSRADANNVYIDVRRRSAAEIGMNASQAGAYDAVFAALGE
                     NADVSTAFLAQNGKDGFLGLYDQMLPDQGEGLFSSLQTANQLISAATGVRPDPGERYG
                     PDSLWIQEINSLVRRDASDTPGSDNQAFGFVAGYEAMGDAGGALGLTLAYINVEEHDI
                     AAKVGEQTTASFVQGGAYWRRSIGGWRLNAGGGGGFGWFEGERRF"
BASE COUNT          527 a         1099 c         1064 g          492 t
ORIGIN      
        1 ccgcgaccac catcagagga tcgggcaggc gctccagaat ggtggcgaag ggcggcagat
       61 cgcgcgccgc caggggcgcc tgggcggcgg cgggcaggtt ttcgatcgcg atccggcgat
      121 ggcgtgcggc cagatacaga cccccgaggc cgctggcgat ggccgccagc caggcgggcg
      181 cgggctgaac cgcccccatt gcggccaggc cgaggatcgt cgcgggtccc accagaacag
      241 gcaggctcga cccgccgatc gagcgggtga aactccgaat ggattgcttc tccggagaca
      301 tggaccgcgc cttcagattc tcgccggtgg ggattcgacg aaccagatat ggcgccgcgc
      361 catgccatgc gatagacggt tggccgctct aatgcgtgtc gatggcgttg ctcaccgcaa
      421 tttcgccgca acgtgacgca agttagggtc tgggttcttc tccgaggtgg ttgtccgtaa
      481 tgcagcgcaa ggtcctggtc gcgacagtcg cagccgctcc tctcctggct ttggcgtttg
      541 gcgcttacgc tgaaacctcg gtcacgacgg cccgcaccac gcccatcgcg acgacgaccg
      601 ccaacaatgg cgcggccgac gacgtgcggg tgacggcgga cggctcgata aagccgacca
      661 cgtccggagc gctgatcacg ctcgacagta accacaaggt caccaacctc gggacgctgg
      721 ccacggttgg cgtcagcgac tcgacgggca tcctgatcct gggtggccgg accggctcgg
      781 tcagcaacgg cgcctcgatc acgatcaacg aagactacac gcccaccgac accgattcgg
      841 acggcgacac cgacggcgcc ttcgcccagg gttccaaccg ctatggcgtg cgcctgaccg
      901 gtccgggagc cttcaccggc aacatcgtca acgaaaccgc cggcgccatc gcgatcgagg
      961 gcaacaattc ggccggcatt tcgctggaaa gcgccctggt cggcaatttc accaataacg
     1021 gctcggtcac cctgctaggc gataacgggg ctggcattcg cattgctgcc cccgtgactg
     1081 gcaaggtcac tgtcaatggc ggcgtgtcgg tggtcggcaa ggacacggtc ggcgtcgcag
     1141 tcgacgccga tgtcagcggc gcctttgtgg ttcagggcgg cgtcagcgcc accggctatc
     1201 gtttcaccac ccggccttcg atcctggccg accgcaacaa gctcgacgct gacgatctgt
     1261 tgcagggcgg ctcggccctg cgtgtgaccg ccaacgtggc cggtggcgtg cttctcgaca
     1321 tcccgcccgc cgacaccgac accaccaaga ccgacgacga cggcgacggc gtcgctgacg
     1381 ccagcgaagg ctctgcatcg ctcgccgcct atggcaaggc cccggccctg ctgatcggct
     1441 ctgacacccg cgccgtgacc gttggcgccg tcgggacggg cgacgacgcc tatggcctgg
     1501 tcgcgcgcgg cgcggtgagc gccagcggcg tctatgacgg tgtcgccggc acggccattc
     1561 agatcggcgg caacgccggc caggccgcca ccctggcggg cggcgccaag ctgtcctcga
     1621 ccgtcgccgc cagcgcctat gaagccaacg ccaccggcgt gcagctgacg gccggcgcca
     1681 gcgtaccgac cctgtggaac cagggctcga tgtcggtctt caccgtgtcg gaaggcgcct
     1741 ttgactcgcg cgccgtcgcc attgacgccg gcgcgtcgat gacgaccttc cgcaatgacg
     1801 ggtcgatcac ctcgacggtc ggcggcgaaa agggcgacgc ctatggcgtc atcgactatt
     1861 cgggcctgct gaccacgatc accaacaacg gcaagatctc gacctcggtg gtcgcgaccg
     1921 acgacgccga ggacaaggac gacgccgaca ccctgcccgg caacgagacg gtcaccggca
     1981 agtccgtcgc catcgatgtc agccgcaaca ccacgggcgt gaccctggtt caggccggcc
     2041 tgagcgacgg cgatgacgcg gcggacggcg tggccgaccc cgatgcggac ggtgatggcg
     2101 tggacgacag cgacgagccg tcgatgctgg gcgcggtccg tttcggctcg ggcgccgaca
     2161 ccttcaacgt gctcaacggc tcggtgattg gcgacatttc gttcggcgcg ggcgccgata
     2221 cgttccggat cgacaatggc tcgctggttc tgggcgcctt gaccgactcc gacggccagc
     2281 tggcgatcac gatcggcgag ggcagcctgg gcctctcgaa cgccgcagtc atcaacgcca
     2341 ccagcctgga tgttggctcg gccggcaaga tcctgttcac cgccgatcca actgcaggga
     2401 ccaacacccg gctgcaggtg tccggcgcgg ccaatatcgc cagcggggcc gagctgggca
     2461 tccgtctgac gggcctggtc aagcagccca cctcctacac ggtgatctcg gccggcagcc
     2521 tgacggtcgg ctcgatcggc cagggcctgt tgtccgactc gccctatctg tacgtggcgt
     2581 ccagccgcgc tgacgccaac aacgtctata tcgatgtgcg ccgccgctcg gccgcagaga
     2641 tcggcatgaa cgcctcgcag gccggcgcct atgacgccgt gttcgccgcc ctgggcgaga
     2701 atgccgacgt gtccaccgcc ttcctggccc agaatggcaa ggacggtttc ctgggcctgt
     2761 atgaccagat gctgcccgac cagggcgagg gcctgttctc gtcgctgcag acggccaacc
     2821 agctgatctc ggcggccacc ggcgtgcggc ccgatccggg cgagcgctat ggccccgaca
     2881 gcctgtggat ccaggaaatc aactcgctgg tccgtcgcga cgccagcgat accccgggct
     2941 cggacaatca ggccttcggc ttcgtggccg gctatgaggc catgggcgac gccggcgggg
     3001 ccctgggcct gaccctggcc tatataaatg tcgaggaaca cgatatcgcc gccaaggttg
     3061 gcgagcagac caccgcctcg ttcgtgcagg gcggcgccta ctggcgccgg tcgatcggcg
     3121 gctggcggct gaacgccggc ggcggcggcg gctttggctg gttcgagggc gagcgccgct
     3181 tc
//
LOCUS       DAOMEO010000266         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3408_length_3181_cov_2.174344, whole genome shotgun sequence.
ACCESSION   DAOMEO010000266 DAOMEO010000000
VERSION     DAOMEO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_3181_cov_2.174344"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PLH31_07245"
     CDS             <1..1556
                     /locus_tag="PLH31_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQR89047.1"
                     /translation="ARARTASEPVYVVTPEEAAAAEKAKATTEKTWIFQASNVRDFGW
                     ASSRKFVWDALGVKQDEPGAEHPVVMAMSFFPKEARPLWDAYSTKSIAHTLKVYSDFT
                     FAYPYPVAQSVNGPVGGMEYPMISFNGPRPVKDKKTGALTYTERAKYGLIGVVIHEVG
                     HNYFPMIVNSDERQWTWMDEGLNSFLEFQAEVQWDKKFPSRRGEPKDIVEYMVSQDQV
                     PLMTQSDSVIQFGPNGYSKPATALVILRETIMGRELFDRAFKEYSNRWRFKHPTPYDF
                     FRTMEESSGVDLDWFWRGWFYSTDHVDISLDKVVKARIESGDPDVEAAVRKARSDVEP
                     ESRTAANNRGTTVIERDPKVRDFYNANDEFAVFDSARKKAKTAREDLTPEEQAAQDLK
                     DNLYRFTFRNIGGLVMPVIVKMDFVDGTSETVRIPAEIWRKNSKQVTWQYVSTKDLKG
                     AQIDPLWETADADRGNNVYEGAITPTTLKIQKPPETTNRMKDANLKVEPGGLGTLPSE
                     EPKKDEGAK"
     gene            1553..2071
                     /locus_tag="PLH31_07250"
     CDS             1553..2071
                     /locus_tag="PLH31_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89048.1"
                     /translation="MIARAAVIIAALGLLLAPLPAAAHRGHGVLSVVEIDAGTGGVSV
                     SHRIPAHDVEPALAQIAPDAQSSLDDPDAVEALKIYMLRTFSLRVNGSVVDLTLRDLT
                     LGADEVRFEYVGQVSPADARAAIEVRAAMFADIHRDEVNQVNIRRLGVTRTLVFNGPD
                     ADTPQALAPIER"
     gene            complement(2233..>3181)
                     /locus_tag="PLH31_07255"
     CDS             complement(2233..>3181)
                     /locus_tag="PLH31_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQR89049.1"
                     /translation="VVEIDAGSLITYSGDLDFYDQQRALSEAQRQAQYERQQAMLAKE
                     INFIEKFKARASHAAQVQSRVKKLDKIERVEAPRRRQSVKFEFQSPPRSGDDVISLRN
                     VHKGYGAQPIYAGLDFLVRRKERWCVLGANGAGKSTLLKLVAGDTKPDQGAVSIGASV
                     RMGYFAQHSMDLLDGEETIFQSMESSFPQAGQGALRTLAGCFGFSGDDVEKPCRVLSG
                     GEKARLVMAKMLFDPPNLLVLDEPTNHLDMATKEMLVAALADFEGTMLFVSHDRHFLA
                     ALSNRVLELTPEGVHQYGGGYTEYVTRTGQEAPGLHPGG"
BASE COUNT          608 a         1025 c         1006 g          542 t
ORIGIN      
        1 tggccagggc ccgcactgcc agcgagccag tctatgtggt gacgccggaa gaggccgcgg
       61 ccgccgaaaa ggccaaggcc accaccgaaa agacctggat cttccaggcc agcaatgtgc
      121 gcgatttcgg ctgggccagc tcgcgcaagt tcgtctggga cgctctgggc gtgaagcagg
      181 acgagcccgg ggccgagcat ccggtggtga tggcgatgtc cttcttcccg aaggaagccc
      241 gcccgctgtg ggacgcctat tcgaccaagt cgatcgccca tacgctgaag gtctattcgg
      301 acttcacctt cgcctatccc tatccggtgg cccagtcggt caacggcccg gtgggcggga
      361 tggagtatcc gatgatcagc ttcaacggcc cgcggccggt gaaggacaag aagaccggcg
      421 ccctgaccta taccgagcgt gccaagtacg gcctgatcgg cgtggtcatc cacgaggtcg
      481 gccacaacta tttcccgatg atcgtcaatt ccgacgaacg ccagtggacc tggatggacg
      541 agggcctcaa cagcttcctc gagttccagg ccgaggtgca gtgggacaag aagttccctt
      601 cgcgccgcgg cgagcccaag gacatcgtcg agtatatggt cagccaggac caggtgccgc
      661 tgatgaccca gtctgactcg gtgatccagt ttggccccaa cggttattcc aagccggcca
      721 ccgccctggt gatcctgcgc gaaacgatca tgggccgcga gctcttcgac cgggctttca
      781 aggaatattc caaccgctgg cgcttcaagc accccacgcc ttatgacttc ttccgcacca
      841 tggaggagtc gtctggcgtc gatctggact ggttctggcg cggctggttc tactcgaccg
      901 accatgtcga catctcgctg gacaaggtcg tcaaggcgcg gatcgagagt ggtgatcccg
      961 acgtcgaagc cgccgtccgc aaggccagaa gcgacgtcga accggaatcc cgcaccgcgg
     1021 caaacaacag gggaacgacg gttatcgagc gcgaccccaa ggtccgcgat ttctacaacg
     1081 ccaatgacga gttcgcggtc tttgacagcg cacgcaagaa ggccaagact gcccgcgagg
     1141 acctgacgcc cgaagagcag gcggcccagg atctgaagga caacctctat cgcttcacct
     1201 tccgcaatat cggcggactg gtgatgccgg tgatcgtcaa gatggatttc gtcgacggga
     1261 cgagcgaaac ggtgcgcatc cccgccgaga tctggcgcaa gaactcaaag caggtcacgt
     1321 ggcagtatgt ctccaccaag gacctgaagg gcgcccagat tgaccccttg tgggaaaccg
     1381 ccgacgccga tcgcggcaac aatgtctacg agggcgcgat cacgccaacg acattgaaga
     1441 tccagaagcc gccggaaacg accaaccgca tgaaggacgc caatctcaag gtcgagccgg
     1501 gcggcctggg aaccttgccg tcggaagagc cgaagaaaga cgaaggcgcc aaatgatcgc
     1561 gcgggcagcc gtcatcatcg cggcgctggg cctgttgctg gccccgctgc ctgcggccgc
     1621 acatcggggc catggcgtgt tgagcgtcgt ggagatcgac gccgggacgg gcggggtcag
     1681 tgtcagccat cgaatcccgg cgcacgacgt agaaccggcc ctggcccaga tcgcgccgga
     1741 cgctcagagc agtctcgacg accccgacgc cgtcgaagct ctgaagatct atatgctgcg
     1801 aaccttctcg ctgagggtca acggctccgt cgttgacctg accctgaggg acctgacgct
     1861 cggcgcggac gaggttcgct ttgagtatgt gggccaagtg tccccggcgg acgctcgcgc
     1921 cgccatcgag gtacgggcgg cgatgttcgc cgacatccat cgcgacgagg tcaaccaggt
     1981 caatatccgt cgcttgggcg ttacgcgaac tctcgtgttc aacggtcccg acgccgacac
     2041 cccccaagcc ttggccccca tagagcgtta ggcctatcta caaggcgcct gatccacagg
     2101 ctcggcgata ccagccgttg gcatgctcga cgctctcagg ggagtccagc ggtcgggcgg
     2161 ccgcccgaca ctatggctct ccaagcatgg acaagcgccg gacgcgcagc ggtggcccgg
     2221 accagggtgg cgctacccgc ccgggtgcag ccccggcgct tcctggcccg tacgcgtgac
     2281 atattccgta tagccgccgc cgtactgatg gacgccctcg ggtgtcagct ccagcacacg
     2341 attcgacagg gccgccagga aatggcggtc gtgtgaaacg aagagcatgg tgccctcgaa
     2401 gtcagccagg gccgcgacca gcatctcctt ggtcgccatg tcgaggtggt tggtcggctc
     2461 gtcgagcacg agcaggtttg gcggatcgaa cagcatcttg gccatgacca ggcgggcttt
     2521 ctcaccgccg gacaagaccc ggcagggctt ttcgacatcg tcgccggaga agccgaaaca
     2581 tcccgccagc gtgcgcagcg cgccctgccc tgcctgcggg aatgagctct ccatggactg
     2641 gaaaattgtc tcttcgccgt ccagcaggtc catggagtgc tgggcgaaat agcccatcct
     2701 gacgctggcc ccgatgctta ccgcgccctg atcgggcttg gtgtcgccgg ccaccagctt
     2761 gagcagcgtc gacttgcccg cgccgttggc gccgagaacg caccagcgtt cctttcggcg
     2821 caccaggaaa tccaggcccg catagatcgg ctgggcgcca tagcccttgt gaacattgcg
     2881 caggctgatc acgtcatcgc ccgaccgtgg cgggctctgg aactcgaact tgaccgactg
     2941 gcgacggcgc ggcgcctcga cgcgttcgat cttgtcgagc ttcttgaccc ggctctggac
     3001 ctgggcggcg tgcgaggcgc gggccttgaa cttctcgatg aagttgattt ccttggccag
     3061 catggcctgc tggcgctcgt actgcgcctg gcgttgcgcc tcgctgagcg cgcgctgctg
     3121 gtcgtagaag tcgagatcgc cggaataggt aatgagcgag ccggcgtcga tctccaccac
     3181 c
//
LOCUS       DAOMEO010000267         3177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3414_length_3177_cov_2.543242, whole genome shotgun sequence.
ACCESSION   DAOMEO010000267 DAOMEO010000000
VERSION     DAOMEO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_3177_cov_2.543242"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1667
                     /locus_tag="PLH31_07260"
     CDS             <1..>1667
                     /locus_tag="PLH31_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89050.1"
                     /translation="INNLATASGSGGRDIQFDIFASEIIQQVTVQKSPRAADEEGGIA
                     GSINISTARPFDYSGQKLVASVEGAHNSISGETDPKVSFLASNTWGDWGALASFSTAQ
                     RTNRTDSNSGINFRPAFRFLEVTGTRGDQAAAVLARDAGVIVKNRRDRNETGRIIFQD
                     KVGDRAYLNSQEQWGGTLSLQYKPSANFDLAFDAMLGRYDSTEDEYDAAAYTASSRST
                     FETIHAYDASTLSDYGMVVLRDVSYTATQHEFLSKEVINETDYAQFGGELNWRGDDWK
                     LHALAGYSGAKKTIDYANLKHVAYAPSRTRWTGRGGETVQSANPATIDMYNAPSKYLF
                     EAYETNYEKITDDKYAAQVDFTKDINLGFFPILKSVQVGARYTDKSKERQYGSLNIQG
                     PGPGSSVWANKRTLAESPLTPINELVPGGDYEVKDITWSQVSNADARRIFRYAGFSTP
                     FNPGQYYEVGEKVSSAYAMADFKADVGPVPVSVNTGVRYVDTSVTSSGYHQIQNPNGS
                     TGYTPAPVSSDGSYKKWLPSLNFTAELTDSLIFRGAASETLIRPALT"
     assembly_gap    1668..1677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1678..2409
                     /locus_tag="PLH31_07265"
     CDS             <1678..2409
                     /locus_tag="PLH31_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89051.1"
                     /translation="KRTASWNSFRFTDGNPNLKPTYAKQWEVGLEKYLPEGGLLSVSY
                     FSKQIEGVVRSALTGTVPNVTKYNANGTIDGVYDFDVYQPVNADGSYDVSGVELVAVV
                     PFGMFYEPLKGFGVNANYTALDSSLTGQSILGIPTPPIGLADTTYNVTLFYENDRFQA
                     RVSYNYKDKYVEGIGYEMYPIWRDGYGQTDMSVSYNINDRFQLSLEAINITDEATTGY
                     TMDPSFPTMYELSGRRAALGLRMNF"
     gene            2619..>3177
                     /locus_tag="PLH31_07270"
     CDS             2619..>3177
                     /locus_tag="PLH31_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR89052.1"
                     /translation="MDAPSPGGGVFARSAGGKAVRQADILIADLRRDLIGYLQRRDRS
                     GDVEDLVQETFVRFHRAGHDLTASDARPLLFVIARNLQLDGWKSTGREKARRTTDDIH
                     DLDAGPRAIAGEEPTADQRLISQQNLAAAAAVIRGLSPKTRDAFLLHRFESLTYRQIA
                     ARLGVSVSMVEKYIAEALRQLKAGRD"
BASE COUNT          631 a         1059 c          932 g          545 t
ORIGIN      
        1 ccatcaacaa tctggcgacc gcttcgggca gcggcggccg tgacatccag ttcgatatct
       61 tcgcctcgga gatcatccag caggtcaccg tccagaagtc gccgcgcgcg gcggacgagg
      121 aaggcggcat cgctggttcg atcaatatct cgaccgcgcg gcccttcgac tacagcggcc
      181 aaaagctggt cgcttcggtc gagggcgccc acaactccat ctccggcgag accgacccga
      241 aggtgtcgtt cctggcgagc aatacctggg gcgactgggg cgctctggcc tcgttctcga
      301 cggcccagcg caccaaccgc actgactcca actccggcat caatttccgt cccgcgttcc
      361 gctttctgga agtgacaggg acgcgaggtg atcaggccgc cgccgtcctg gcgcgcgacg
      421 ccggcgtcat cgtcaagaac cgtagggatc gcaacgagac cggccggatc atctttcagg
      481 acaaggtcgg ggaccgcgcc tatctcaaca gccaggaaca atggggcggc accctgtcgc
      541 tgcagtacaa gccctcggcc aatttcgatc tggcgttcga cgcgatgctg ggccgctatg
      601 actccaccga ggacgagtac gacgctgcgg cctatacggc ctccagccgc agcaccttcg
      661 agacgatcca cgcctatgac gcctccaccc tgtccgacta tggcatggtc gtgctgcgcg
      721 acgtctccta caccgccacc cagcacgagt tcctgagcaa ggaagtcatc aacgagaccg
      781 actacgccca gttcggcggc gaactgaact ggcgaggcga cgactggaag ctgcacgccc
      841 tggcgggcta ttccggcgcc aagaagacga ttgactacgc caacctcaag cacgtggcct
      901 atgcgccgtc gcgcacccgc tggacgggcc gtggcggtga aaccgtgcag agcgccaatc
      961 cggcgacgat cgacatgtac aacgcgccgt cgaagtacct gttcgaggcc tatgagacca
     1021 actacgagaa gatcaccgac gacaaatatg cggctcaggt cgacttcacc aaggacatca
     1081 atcttggctt cttcccgatc ctgaagagcg ttcaggtcgg cgcgcgctac acggacaaat
     1141 ccaaggaacg ccagtatggc tccttgaaca ttcaaggtcc gggaccgggc agctcagtct
     1201 gggccaacaa gcgcaccctt gctgagagcc cgctgacccc gatcaacgag ctggtcccag
     1261 gtggcgacta tgaggtcaag gacatcacct ggagccaggt gtccaacgcg gatgcgcgcc
     1321 gcatcttccg ctacgcgggc ttctccacgc cgttcaatcc gggccagtat tatgaggtcg
     1381 gggaaaaggt ctccagcgcc tacgccatgg ctgacttcaa ggccgatgtc ggtccggttc
     1441 cggtctcggt caacaccggc gttcgctatg tggacacctc ggtgacctcc tcgggctatc
     1501 accagatcca gaatccgaac ggctcgaccg gctacacccc ggcgcccgtg tcgagcgacg
     1561 gcagctacaa gaagtggctc cccagcctca acttcaccgc cgagctgacc gacagcctga
     1621 tcttccgcgg cgctgcgtcg gaaaccctga tccgaccggc gctgaccnnn nnnnnnnaag
     1681 cgcacggcca gctggaactc gttccgcttc acggacggca acccgaacct gaagcccacc
     1741 tatgccaagc agtgggaagt gggtctcgag aagtacctgc cggaaggtgg actgctatcg
     1801 gtgtcctact tctccaagca gatcgaaggc gtggtccgca gcgctctgac cggcaccgtg
     1861 ccgaacgtca ccaagtacaa cgccaacggc acgatcgacg gcgtctacga ctttgatgtc
     1921 tatcagccgg tcaacgccga tggctcctat gacgtcagcg gcgttgaact ggtcgccgtc
     1981 gtgccgttcg gcatgttcta cgagccgctg aagggcttcg gcgtcaacgc caactatacg
     2041 gcgctagata gttcgctgac cggccaatcg atcctgggca tcccgacgcc gccgatcggg
     2101 ctggccgaca cgacctacaa cgtcacgcta ttctacgaga acgaccgctt ccaggcgcgc
     2161 gtgtcctata actacaagga caagtacgtc gagggcatcg gctacgagat gtatccgatc
     2221 tggcgcgatg gctatggcca gaccgatatg tcggtcagct ataacatcaa cgaccgtttc
     2281 cagctcagcc tagaagcgat caacatcacc gacgaggcga ccaccggcta cacgatggat
     2341 ccgtcattcc cgacgatgta cgaactgtcg ggacgccgcg cggcgctcgg cctgcggatg
     2401 aacttctgat ccacggtgca ctggggatgg ctcgcgtgaa ccgaccatcc cctttccttt
     2461 gacgggccag aggccatcct gaaaaactgt catgtttttc agccatggct gggccgccat
     2521 caaggcgatg aggaactatg gcggcaggtc caaaagcatg gacgcagccc ccatcccgaa
     2581 cctcggacgc gttccttatc cccgagcgcg accgtcgtgt ggacgctccc tccccaggag
     2641 ggggcgtctt tgcgcgttcg gcgggaggca aggcggttag gcaggccgat atcctgatcg
     2701 ctgacctgcg ccgcgacctg attggctacc ttcagcgtcg tgaccggtcc ggggatgtcg
     2761 aggatctggt ccaggagacc tttgtccgct ttcaccgggc cggccacgac ctgaccgcgt
     2821 ccgacgcccg cccgttgctc ttcgtgatcg cccgcaacct gcagctcgac ggctggaaat
     2881 ccacgggacg cgagaaggcc cgtcgtacca ccgacgacat ccacgacctg gacgcagggc
     2941 cacgcgcgat cgctggcgag gagccgaccg ctgatcagcg cctgatctct cagcagaacc
     3001 tggccgccgc cgccgccgtg attcgcggcc tgtcgccgaa gacccgagac gccttcctgc
     3061 ttcaccgttt cgagagcctg acctaccgtc agatcgccgc gcgactcggc gtttcagtca
     3121 gcatggttga aaagtatatc gccgaagcgc tgcggcagct gaaggccggg cgggatg
//
LOCUS       DAOMEO010000268         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3417_length_3174_cov_2.361654, whole genome shotgun sequence.
ACCESSION   DAOMEO010000268 DAOMEO010000000
VERSION     DAOMEO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_3174_cov_2.361654"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..2116
                     /gene="typA"
                     /locus_tag="PLH31_07275"
     CDS             284..2116
                     /gene="typA"
                     /locus_tag="PLH31_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQR89053.1"
                     /translation="MSMRNIAIIAHVDHGKTTLVDQLLAQSGIFRANEATTERAMDSN
                     DQERERGITILAKCTSVLWNGEAGETRINIIDTPGHADFGGEVERILGMVDGCVILVD
                     AEEGVMPQTKFVLGKALKMGLRPILCLNKVDRPHADPDRILNDVFDLFAAMGATDEQL
                     DFPHIYASGKNGWATLDMNQPNSTLAPLFDLIVRHVPEPKVVANVDKPFQILSVLIEN
                     DPFLGRLLTGRITSGKAVPGMAIHALSLDGKEIERGRITKVLAFRGLKRQPVDEAEAG
                     DIVAIAGLSKATVADTLCAMEVTEALPAQPIDPPTISMTVSVNDSPLAGKEGDKVQSR
                     VIRDRLLREAESNVAIKVTESAEGDAYEVAGRGELQLGVLIEGMRREGFEVSISRPRV
                     VFQTDPETGKRLEPIEDVMIDVDDEFSGIVIEKLSQRKAELKDMGPSGAGKTRIQLTA
                     PSRSLIGYQGEFLTDTRGSGVLNRVFSHYEPYKGAIDSQRKGVLVSNSDGETAAFALW
                     NLEDRGTMFVGAGEKTYQGMIIGENARSDDLDVNPMKAKQLNNIRASGKDEAVRLTPP
                     RRMTLEQAIAYIEEDELVEVTPKNIRLRKQTLNPSFRKRRVKEE"
     gene            2306..>3174
                     /locus_tag="PLH31_07280"
     CDS             2306..>3174
                     /locus_tag="PLH31_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR89054.1"
                     /translation="MLKAAASSITAGRLNALFASMLPAVPEEMAGRVRMEQLVSILRL
                     TPIMMGANIVIAILVCLTGLAGPHRIALTIWSALVISYGLLGLRGWVAAQRRKSGKTT
                     VSARGLGRIALQAGLLGCLWGALPMLAMDDDAASVQMRMMIVSVIAGMIGCGGFAMLA
                     LPPAAIAYSTPMVIGSLFVLVRSGDPILLALGGLLIFCYLVVSFSCLSHAKVFAERLV
                     AGEDLERQKQVVSLLLSDFEEGASDWLWEIDDQGALTYVSDRMAAAAGVSRSDLIGRP
                     LAALCGAAAESPAG"
BASE COUNT          592 a         1067 c          987 g          528 t
ORIGIN      
        1 aaagagaaac cctttccgga gcagggatta tcgaacacca atctgccata accgctactt
       61 tttgaggcat gctgcagtgc acaattagtt gcatgcgaaa caactccatc cgttgcggcc
      121 cctcaaaccc cacggaatca agggtttcag cttgacaccg gtttccacga aagcgccttt
      181 tgcaagaggt ggggtggcta aacgcagcgc agtcgcgtaa aagccgcgcg cctgacgcgc
      241 gcagcgtcaa acccgttctc ttccccgaaa gttcgccttc tccatgtcta tgcgaaacat
      301 cgccatcatc gcgcacgtcg atcatggcaa gaccacgctc gtcgaccagc tgctcgccca
      361 gtccggcatc ttccgcgcga atgaagccac gactgaacgc gccatggact ccaatgacca
      421 ggagcgcgag cgcgggatta ccatcctggc caagtgcacc agcgtcctgt ggaacggtga
      481 ggcgggcgag acccgcatca acatcatcga cacccccggc cacgccgact tcggcggcga
      541 agtcgaacgt atcctcggca tggtcgacgg ctgcgtcatc ctggtggacg ccgaagaggg
      601 cgtcatgccc cagaccaagt tcgtcctggg caaggccctg aagatgggcc tgcgtccgat
      661 cctctgcctc aacaaggtgg accgtccgca cgctgatccg gaccggatcc tgaacgacgt
      721 gttcgacctg ttcgccgcca tgggcgccac ggacgagcag ctggacttcc cgcacatcta
      781 tgcgtcgggc aagaacggct gggccacgct ggacatgaac cagcccaaca gcaccctggc
      841 tcccctgttc gacctgatcg tgcgccacgt gcccgagccc aaggtcgtcg ccaacgtcga
      901 caagccgttc cagatcctgt cggtcctgat cgaaaacgac cccttcctgg gtcgcctgct
      961 gaccggccgc atcacctcgg gcaaggccgt ccccggcatg gccatccacg ccctgtcgct
     1021 ggacggcaag gaaatcgagc gcggccgcat caccaaggtt ctggccttcc gcggcctgaa
     1081 gcgccagccg gtcgatgaag ccgaagccgg cgacatcgtc gccatcgcgg gcctgtccaa
     1141 ggccaccgtg gccgacaccc tgtgcgccat ggaagtcacc gaggccctgc ccgcccagcc
     1201 gatcgatccg cccaccatct cgatgaccgt ctcggtcaac gacagcccgc tggcgggtaa
     1261 ggaaggcgac aaggtccaga gccgcgtcat tcgcgaccgc ctgctgcggg aagccgaatc
     1321 caacgtcgcc atcaaggtga ccgagtcggc cgagggcgac gcctacgaag tcgccggtcg
     1381 cggcgaactg cagctgggcg tgctgatcga aggcatgcgc cgcgaaggct tcgaagtgtc
     1441 gatcagccgt ccgcgcgtgg tcttccagac cgatccggaa accggcaagc gccttgagcc
     1501 catcgaagac gtcatgatcg acgtcgacga cgagttctcg ggcatcgtca tcgaaaagct
     1561 gtcgcagcgt aaggccgagc tgaaggacat gggtccgtcc ggcgccggca agacccgcat
     1621 ccagctgacc gccccgtcgc gttcgctgat cggctatcag ggcgagttcc tgaccgacac
     1681 ccgcggttcg ggcgtgttga accgcgtgtt cagccactac gagccctaca agggcgccat
     1741 cgacagccag cgcaagggcg tgctggtttc gaactcggac ggcgaaaccg ccgccttcgc
     1801 gctgtggaac ctggaagatc gcggcacgat gttcgtcggc gccggcgaaa agacctatca
     1861 gggcatgatc atcggcgaga acgcccgttc ggacgacctc gacgtcaacc cgatgaaggc
     1921 caagcagctg aacaacatcc gcgcctcggg caaggatgaa gccgttcgcc tgaccccgcc
     1981 gcgccgcatg accctcgaac aggccatcgc ctatatcgag gaagacgagc tggtcgaagt
     2041 gacgcccaag aacatccgcc tgcgcaagca gaccctgaac ccgtcgttcc gcaagcgtcg
     2101 cgtcaaggaa gaataggact tcacgtcctg atctgacaaa ggccgccgcc cctttcggga
     2161 cggcggcctt tttgtttggg gcgaagggcg cgtcgcgacc aaggggcgca tcaagacacc
     2221 cggcaaactt aacgacgagt ttacgataac gggagatggt caccgccggg atgttgtcgt
     2281 cccgggggaa agccaggacc gcgccgtgct caaggccgcc gctagctcga tcacggcagg
     2341 gcgtttgaac gccctgttcg ccagcatgct cccggcggtg cccgaggaga tggccgggcg
     2401 cgtgcgcatg gagcaactgg tcagcatcct gcgcctgacg cccatcatga tgggcgccaa
     2461 tatcgtcatc gccatcctgg tttgcctgac cggcctggcg ggcccgcatc gcatcgcgct
     2521 gacgatctgg tccgccctgg tgatcagcta tggcctgctg ggcctgcgcg gttgggtggc
     2581 cgcccagcgc cgcaagagcg gcaagaccac cgtctcggcc cgcggcctgg gccggatcgc
     2641 cctgcaggcg ggtttgctgg gctgcctttg gggcgccctg ccgatgctgg ccatggatga
     2701 cgacgccgcc agcgttcaga tgcggatgat gatcgtctcg gtgatcgccg gcatgatcgg
     2761 ctgcggcgga tttgcgatgc tggccctgcc acccgccgcc atcgcctatt cgaccccgat
     2821 ggtgatcggc tcgcttttcg tgctggtgcg cagcggcgac ccgatcctgc tggccttggg
     2881 cggtcttctg atcttctgct acctggtcgt cagcttctcc tgcctgtcgc acgccaaggt
     2941 ctttgctgaa cgcctggtgg ccggcgaaga cctggaacgc cagaagcagg tcgtcagcct
     3001 gctgctcagc gacttcgagg agggggccag cgactggctt tgggaaatcg acgatcaggg
     3061 cgccctgacc tatgtgtccg acaggatggc cgccgccgcc ggagtcagcc ggagcgatct
     3121 gatcggccgg ccgctggcgg cgctgtgcgg agcggccgct gaaagccccg ccgg
//
LOCUS       DAOMEO010000269         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3432_length_3165_cov_2.584566, whole genome shotgun sequence.
ACCESSION   DAOMEO010000269 DAOMEO010000000
VERSION     DAOMEO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_3165_cov_2.584566"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLH31_07285"
     CDS             <1..305
                     /locus_tag="PLH31_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR89055.1"
                     /translation="ELSLTGNFLDAKTAAEWGLVNRVVPAADLMPTALKLAADMASIP
                     VETLSFYKSLIDEGFERSFRKGLEIEHERSSAHNRKVTPGAVEARRAQIQARGRTQ"
     gene            complement(308..1267)
                     /locus_tag="PLH31_07290"
     CDS             complement(308..1267)
                     /locus_tag="PLH31_07290"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HQR89056.1"
                     /translation="MLTRRIVTAGLGLSFMTPGVFAQEPAPATKPVESPEPVLILDAS
                     LDPDDRLTVEVTLNGVGPFQFIVDSGAGRSVLSETLANRLALAQGPDILVHGISGSII
                     ARTAKVAQLMVGDARLSNVNLPVLPPERVGADGFLGVDILEGRNVIMDFRKRKLEVRR
                     SRSPFDLVRLPREVSVMADDRFGRLTVADCRVAGARALAFVDSGGGVSIGNKALSRAI
                     ATRRRRHDDVRSAQLLTASGEVTVGEYRIAPSLTLGDLRMTNVPMAFADLHIFDVWGL
                     NDRPALLLGVDILKLFSRVELDFGAGRVLFRIGGVTPPRVLSA"
     gene            complement(1383..1565)
                     /gene="rpmF"
                     /locus_tag="PLH31_07295"
     CDS             complement(1383..1565)
                     /gene="rpmF"
                     /locus_tag="PLH31_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQR89057.1"
                     /translation="MAVPKRKTSPSRRNMRRSHHALGANSFVEDKDSGELRRPHHVDL
                     KTGLYNGKQILTPKED"
     gene            complement(1681..>3165)
                     /locus_tag="PLH31_07300"
     CDS             complement(1681..>3165)
                     /locus_tag="PLH31_07300"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQR89058.1"
                     /translation="GEVFVTDDGAETDLDLGHYERFTGVNATKADNITTGQIYKTIIE
                     KERRGDYLGATVQVIPHVTNEIKQFVLSPAMDETGEKPVDFVLVEIGGTVGDIEGLPF
                     FEAIRQLRQDLPRGQSCYVHLTLLPFIKTAGEMKTKPTQHSVKELRSIGIQPDILLCR
                     CEQEIPVEEKRKIAQFCNVRPSAVIQAMDSSSIYAVPIDYHQEGLDAEVLDVFGLHDA
                     PKPDLSRWEAIDQVVNHPDGEVTIAVVGKYTVLKDAYKSLIEALHHGGLANKVKVNLD
                     WIESETFEGDPGAAAVRLENAHAIMVPGGFGERGAEGKIRAAQFARERKVPYFGICFG
                     MQMAVIETLRNVAGITDASSSEFGPTDRPVVGIMTEWIKGNETVQRRSGDDLGGTMRL
                     GAFDAVLTPGSKVAAIYGDTAISERHRHRYEVNIGYSHRMEESGLKLTGRSPDGVLPE
                     IVERDDHPWFIGVQFHPELKSRPFAPHPLFASFIAAAKEQGRLV"
BASE COUNT          567 a          998 c         1028 g          572 t
ORIGIN      
        1 aggaactgtc gctgaccggc aatttcctgg acgcgaagac cgctgccgag tggggccttg
       61 tcaatcgggt ggtccctgcc gctgacctga tgcccacagc tctgaaactg gccgccgaca
      121 tggcctcgat cccggtggag accctgtcgt tctacaagag cctgatcgac gagggcttcg
      181 agcggtcttt ccgcaagggt ctggagatcg agcatgagcg atccagcgct cacaatcgca
      241 aggtcacgcc gggagcggtg gaagcgcggc gggcgcagat tcaggcccgg ggacgcaccc
      301 agtagggtca ggcgctaagg acgcggggcg gcgttacacc gccgatgcgg aacagcacgc
      361 gccccgcgcc gaagtcgagt tcgacccggg aaaacagctt caggatatca acgcccagca
      421 gcagggctgg ccgatcatta agcccccaga catcgaagat gtgcaggtcg gcaaaggcca
      481 tcggcacgtt ggtcattctc agatcgccca gggtcaggga tggcgcgatc cgatattcgc
      541 ccaccgtgac ctcgcccgag gccgtcagca actgggccga cctgacatcg tcgtggcgtc
      601 gccgtcgcgt ggcgatggct cgagacagcg ccttgttgcc aatactgacg ccaccgccgg
      661 aatcgacaaa ggccagggcc ctcgccccgg ccacccggca gtcagcgacg gtcaggcggc
      721 cgaaccggtc gtcggccatg accgagacct cgcgtggcaa tctgacaaga tcaaacggtg
      781 accggctccg acgcacctca agcttgcgct tgcggaagtc catgatgacg ttgcggcctt
      841 caagaatatc gacgcccaga aagccgtccg cgccaacccg ctctggaggc agcacgggta
      901 gattgacgtt cgacagccgc gcgtcgccaa ccatcagctg ggcgaccttc gccgttcggg
      961 cgattatcga gccgctgatg ccgtgaacga gaatgtcggg cccttgcgcg agggccagcc
     1021 gattggcgag cgtctcagac aacacgctgc gaccggcgcc gctgtcgaca atgaactgga
     1081 agggccccac gccattgagc gtgacctcga ccgtcaaccg gtcgtcggga tcgagagagg
     1141 cgtcaagaat gagaacgggt tcgggagact cgacaggttt ggtcgctggc gcaggctcct
     1201 gggcaaacac cccgggggtc atgaacgaaa gccccagtcc cgccgttacg atgcggcgtg
     1261 tgagcatggg cggacctccc tggtccgctc acgataacac cgttttttag acggccatga
     1321 cgaaaaacgc cgccaggcga accgggcggc gtcttcgcgc tcacagcctt gaaggagaga
     1381 ccttagtctt ccttgggggt gaggatctgc ttgccgttgt acaggcccgt cttcaggtcg
     1441 acgtggtggg gacgacgcag ttcgcccgag tccttgtctt cgacgaagga gttggcgccc
     1501 agggcgtggt gcgaccggcg catgttccgg cgagaaggcg aagtttttct tttgggaacg
     1561 gccatcgaag tgactctgcg cgctgagcgt gaaaattaga gacggcgact cgggaagacc
     1621 caggccgccg tgtgagcgcg cgcttatgcc tgaatctctt cagacatgca acacgcctgt
     1681 ttagactagc ctgccctgct cttttgccgc tgcaatgaag ctggcgaaca gcggatgcgg
     1741 ggcgaatgga cggcttttca gctccgggtg gaactgcacg ccgatgaacc acggatggtc
     1801 gtcgcgttcg acgatctcgg gcagcacgcc gtcgggcgaa cgaccggtca gcttcaggcc
     1861 ggactcctcc atccggtggc tgtagccgat attgacctcg tagcggtgcc tgtggcgttc
     1921 gctgatggcc gtgtcgccat agatggccgc caccttgctg ccgggcgtca ggacagcgtc
     1981 gaaggccccc aggcgcatgg tgccgcccag gtcgtcgccc gagcgccgct ggaccgtctc
     2041 attgcccttg atccactcgg tcatgatccc gacgactgga cggtcggtgg ggccaaactc
     2101 gctggacgag gcatcggtaa tgccggccac gttgcgcagg gtttctatga ccgccatctg
     2161 catgccgaaa cagatgccaa aatacggcac cttgcgttcg cgggcgaact gggcggcgcg
     2221 gatcttgccc tcggcgcccc gctcgccaaa gccgccgggc accatgatgg catgagcgtt
     2281 ttccagacga accgcagccg cgccgggatc gccctcgaag gtctcgctct cgatccagtc
     2341 gagattgacc ttcaccttgt tggccagacc cccgtgatgc agggcctcga tcagcgactt
     2401 ataggcgtcc ttcaggacgg tgtacttgcc gaccacggcg atggtcactt cgccgtcggg
     2461 atgattgacg acctggtcga tggcttccca gcggctcagg tccggcttgg gcgcgtcgtg
     2521 caggccgaac acgtccagca cctcggcgtc gaggccttcc tggtgatagt cgatcggcac
     2581 cgcatagatc gaagacgagt ccatcgcctg gatcacggcg ctgggccgca cgttgcagaa
     2641 ctgcgcgatc ttgcgctttt cctcgaccgg aatttcctgc tcgcaacggc acagcaggat
     2701 gtcgggctgg atgccgatcg agcgcagttc cttgacggag tgctgggtcg gcttcgtctt
     2761 catctcgccg gccgtcttga tgaacggcag cagggtcaga tggacatagc agctctggcc
     2821 gcgcggcagg tcctggcgca gctggcggat ggcctcgaag aacggcaggc cctcgatgtc
     2881 gccgaccgtg ccgccgatct cgaccagcac gaagtcgacc ggcttctcgc ccgtctcgtc
     2941 catggccggc gacagcacga actgcttgat ctcgttggtg acgtgcggaa tcacctggac
     3001 ggtcgcgccc agatagtcgc cgcgccgctc cttctcgatg atggtcttgt agatctggcc
     3061 ggtggtgatg ttgtcggcct tggtggcgtt caccccggtg aagcgctcgt agtggcccag
     3121 gtccaggtcg gtctcggcgc cgtcgtcggt cacgaagacc tcgcc
//
LOCUS       DAOMEO010000270         3156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3440_length_3156_cov_2.486295, whole genome shotgun sequence.
ACCESSION   DAOMEO010000270 DAOMEO010000000
VERSION     DAOMEO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_3156_cov_2.486295"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLH31_07305"
     CDS             <1..313
                     /locus_tag="PLH31_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQR89059.1"
                     /translation="GLIAALQVLAVMIETQLPMSALARQFEPVPQLLENVRFTGGKPL
                     EAKTVKEAIADGESQLNGAGRIVVRASGTEPLIRIMAEGDDPALVRKVVKSIVSAVKA
                     A"
     gene            complement(376..1065)
                     /locus_tag="PLH31_07310"
     CDS             complement(376..1065)
                     /locus_tag="PLH31_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HQR89060.1"
                     /translation="MTSPAIDLTVHHKPAGASDRVAYGFVKLLRFFADTFFAKRYGHR
                     AVVLETVAAVPGMVGATLNHLKCLRRMEDDKGWIKTLMDEAENERMHLMTFIQVAQPT
                     AFERFVVVAAQWVFYLFFFALYLVSSKTAHRVVGYFEEEAVISYTHYLAEIDEGRSEN
                     VAAPQIALDYWDLPAGATLRDVVEVVRADEAHHRDVNHGFANELCGLPHGVIAPCPPH
                     VVLEPNWKTAA"
     assembly_gap    1174..1183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1219..2952)
                     /locus_tag="PLH31_07315"
     CDS             complement(1219..2952)
                     /locus_tag="PLH31_07315"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQR89061.1"
                     /translation="MTIQRLLIPLAVGVLLAAPAARAAEPAPEKTFWGTETAEAAALQ
                     SLYATKQGTMGVASRAMVAACHDRSVEIGLDILRRGGSATDAFIAVTFADYVQTPGAS
                     SLAGPMGALIHDPRTGEVHSLAAPLKTVSSLGGQWTTGETAVGKQVLVPGAVAGLEAM
                     HRRFGRLPWADLVRPAAEMAREGFPVTPLMSGISIAYKATLSASDYGRQTYLRPDGSP
                     HPVGAILKLPALADTLDAIAAKGAAHVQTGAWAQAVVAMVKEKGGEMTAADLADYRPE
                     WSAPLRIAYRGRVIHGLGGHNSGGARLLLALETLEQADIKALGHPSESLDGLEAMVRI
                     SRAVSAEPPLNTHAFFEDPKAPAALLAGSRPAQIWGEVAARIQRPPLPAPGSHSYAVA
                     VVDEQGGAVTGTHTIESLPFGTNGLFVGGVPLNNTGIQHPYSADSAYATPPGRYLIEP
                     LSAVLAFEGDRLVLAGGTFSASLWPADFQMVSSLLDFDWTPERVALTPRFGGYGIDLT
                     KLTADISVNVIDKRVRAEVVEAMKQRGVALSQAGYVDTGMLVMIKRDLRSGALTGFTP
                     EALPEGRAAGY"
     gene            complement(3041..>3156)
                     /locus_tag="PLH31_07320"
     CDS             complement(3041..>3156)
                     /locus_tag="PLH31_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR89062.1"
                     /translation="VGKKPRTRAALTPTGLKAFRAHVDYLREVVEEVGDAP"
BASE COUNT          501 a         1035 c         1128 g          482 t
ORIGIN      
        1 tggtctgatc gcggccctgc aggtgcttgc ggtgatgatc gagacgcaac tgccgatgag
       61 cgctctggcc cgccagttcg agcccgtgcc gcaactgctg gaaaacgtcc gcttcaccgg
      121 cggcaagccc ttggaagcca agacggtgaa ggaagccatc gctgacggcg agagccagct
      181 gaacggcgcc ggacgcatcg tggtgcgcgc ctcgggcacc gaacccctga tccgcatcat
      241 ggccgagggc gacgacccgg ccctggtccg caaggtggtc aagtcgatcg tctcggccgt
      301 gaaggccgcc tgaaacgcaa aacgccccga ggcgaggcct cggggcgtac gcggatcctc
      361 agcgggctgg cggcgtcagg ccgccgtctt ccagttcggt tccagcacca cgtgcggcgg
      421 gcagggcgcg atcacgccat ggggcaggcc gcacagttcg ttggcgaagc catggttgac
      481 gtcgcgatgg tgggcttcat cggcgcgaac cacctcgacc acgtcgcgca gggtggcgcc
      541 tgccggcagg tcccagtagt cgagcgcgat ctgcggcgcc gccacgttct cggaacggcc
      601 ctcgtcgatc tcggccagat agtgggtgta gctgatcacc gcctcttcct cgaaatagcc
      661 gaccacgcgg tgggcggtct tggacgacac cagatagagc gcgaagaaga acaggtagaa
      721 cacccactgg gcggcgacga ccacgaagcg ctcgaaggcg gtcggctggg cgacctggat
      781 gaaggtcatc aggtgcatcc gctcgttctc ggcctcgtcc atcagggtct tgatccagcc
      841 cttgtcgtct tccatccggc gaaggcactt caggtggttc agcgtggcgc cgaccatgcc
      901 gggcacggcg gccacggttt ccagcaccac cgcgcggtgg ccatagcgct tggcgaagaa
      961 ggtgtcggca aagaagcgca gcagcttcac aaagccatag gccacgcggt cagaggcgcc
     1021 ggcgggcttg tggtggacgg tcaggtcgat ggctgggctg gtcatggcgg tggttccggt
     1081 cggatcggcc cgcaccgggc cgctgatgac cgacttgata cgccgtcctc acggcgcatg
     1141 acattcgggg ccacccccta gagaagtccc cctnnnnnnn nnngggacga tacctagctg
     1201 accgagcgta ttgactggct agtagcccgc cgcgcgtcct tcgggcagcg cctcgggcgt
     1261 gaagcccgtc agggcgccgg agcgcaggtc gcgcttgatc atcaccagca tgccggtgtc
     1321 gacatagccg gcctgggaca gggcgacacc ccgctgtttc atcgcctcga ccacctcggc
     1381 cctcacccgc ttgtcgatga cattgaccga aatgtcggcg gtcagcttgg tcagatcgat
     1441 cccatagccg ccgaagcgcg gcgtcagggc gacgcgttcc ggcgtccagt caaagtccag
     1501 caggctggag accatctgga agtcggctgg ccatagcgag gccgagaagg ttccgccggc
     1561 gagcaccaga cgatcgccct cgaaggccag aaccgccgac agcggctcga tcagatagcg
     1621 tcccggcggg gtcgcatagg cggagtccgc gctgtagggg tgctgaatgc ccgtgttgtt
     1681 gagcggcacg ccgccgacaa agaggccgtt ggttccgaag ggcagggact cgatggtgtg
     1741 ggtgcccgtg actgcgccgc cctgctcgtc caccacggcg acggcgtagc tgtgcgatcc
     1801 gggggcgggc agcggcggac gctggatcct ggcggcgacc tcgccccaga tctgggcggg
     1861 ccgcgagccg gccagcaggg ccgcgggcgc cttcgggtcc tcgaagaagg cgtgggtgtt
     1921 gaggggcggc tcggcgctga ccgcccggct gatccgcacc atggcctcca gtccatcgag
     1981 agactcagac ggatggccca gggccttgat gtccgcctgc tccagggtct ccagcgccag
     2041 cagcaggcgc gcgccgccgc tgttgtggcc gcccaggcca tggatgacgc gaccgcgata
     2101 ggcgatgcgc agcggcgcgc tccattcggg gcgatagtcg gccaggtcgg cggcggtcat
     2161 ctcgccgccc ttttccttca ccatcgccac gaccgcctgg gcccaggcgc cggtctggac
     2221 atgggccgcg cccttggcgg cgatggcgtc cagcgtgtcg gccagggccg gcagcttcag
     2281 gatggcaccg acggggtgtg gcgatccgtc cggccgaaga taggtctgcc ggccatagtc
     2341 cgacgcgctc aaggtggcct tgtaggcgat gctgatcccg gacatcagcg gcgtaacggg
     2401 aaagccctcg cgggccatct cagccgcggg ccgcaccaaa tccgcccagg gcaggcggcc
     2461 aaagcgccgg tgcatcgcct ccaggcccgc gacggcgccg gggaccagaa cctgcttgcc
     2521 gacagccgtt tcgccggtcg tccactgacc gccgagactg ctcacggtct tcaggggcgc
     2581 ggccaggctg tgaacctcgc cggtccgggg atcatggatc agggccccca tggggccggc
     2641 gagactgctg gctccgggag tctgcacata gtcagcgaag gtgacggcga tgaaggcgtc
     2701 ggtcgccgag ccgccccgcc gcaggatgtc cagcccgatc tcgacagatc ggtcgtgaca
     2761 ggcggccacc atggcccggg aagcaacgcc catggtccct tgcttggtcg catagagcga
     2821 ctgcagggcg gccgcctcgg cggtttcggt tccccagaag gtcttttccg gggcaggctc
     2881 ggcggcgcgc gccgccggtg cggcgagcag gacgccgacc gcgaggggga taagcaagcg
     2941 ctggatcgtc atggcggtgc tcctgtgggg gcggcggcca ggatttgggc gcatgaggcg
     3001 ccgtacggat cgccgcgtgt gggaatggcc cgggttgggc ctagggggcg tcgcccacct
     3061 cctcgaccac ctcgcgcaga tagtcgacat gggcgcggaa ggcctttagg ccggtcggcg
     3121 tcagggccgc gcgggtgcgg ggcttcttgc cgacat
//
LOCUS       DAOMEO010000271         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3447_length_3152_cov_2.894737, whole genome shotgun sequence.
ACCESSION   DAOMEO010000271 DAOMEO010000000
VERSION     DAOMEO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3447_length_3152_cov_2.894737"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLH31_07325"
     CDS             complement(<1..383)
                     /locus_tag="PLH31_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HQR89063.1"
                     /translation="MIHGLVRRRRRRLLVIAAALAPAVLLGALALVVQGPSLLWNRSE
                     SEPRGLYIRSAAAPSRGKIIAFPAPPSVLTYASGRMAYLRHMPILKEVAAMSGDTVCT
                     LDGKLVINGVWRAPVLAHDSERRPLP"
     gene            complement(1170..1832)
                     /locus_tag="PLH31_07330"
     CDS             complement(1170..1832)
                     /locus_tag="PLH31_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR89064.1"
                     /translation="MSVISVCSTKGGVGKTTLVMCLADAFARQGGSTAIVDGDPNGHV
                     ATWRERAGDECKVTVISEADETKIQDVIAQAAAKYALVVVDLEGAASQAVTYAIAESD
                     LVLIPTKISGMDLQEVFRTYEVVKRAEKMLKRPIPARVVLTQMSPLQSRVAQHARSEI
                     QANSIPVLKTEIIQRAAYQSIHFTGATPAHEGGDAKAAREITAVLGEILEALAAHAQA
                     AA"
BASE COUNT          543 a         1007 c         1045 g          557 t
ORIGIN      
        1 ggtagcggcc ggcgttcgct gtcgtgggcg agcacgggcg cgcgccacac gccgttgatc
       61 accagcttgc catcgagggt gcagacggtg tctcctgaca tcgccgcgac ctccttcagg
      121 atcggcatat ggcgcagata ggccatgcgg ccgctggcgt aggtcaggac cgagggcggg
      181 gctgggaacg cgatgatctt gccccggctt ggagccgcgg cgctgcggat gtagaggccc
      241 cggggctcac tctccgagcg gttccacagc agcgacgggc cctgaacgac cagagccagg
      301 gcgcccagca gcacagccgg agccaatgcc gccgcgataa ccaggagcct gcgtcgccgg
      361 cgtctcacaa ggccatggat catgtctcag ccctatgttg acgccgcctg cttgcgatca
      421 gccacggggc gggcgcactg gaagaccgcg cagattgcgc ttcctttgag tgccttttat
      481 ggcaaaaagt cccctgccct accagagctg acgcatcggt tcttgggtgg cgcggatcgc
      541 gcgatcggga caggaccgcg caaggtgcgg gcgttcgccg gcatactcgc aggccgccgc
      601 tcccgatccg aagccaatgg ggcaaagggg tgtttgcccc attggccgta ggtcatcttc
      661 ctatccgccc ggccgcttgg ggctaggctg aaagtccccg gtcggggcta acgcaggcgc
      721 cggccatctt ccgggaccgc gctcaggtcg acctggtagc ccttgtccgc caacgcttcc
      781 aagacgaggt atttgaccgt cacacgccgg ttcaaggcct catgccggag agcttgccaa
      841 acggcatctg gaacatcgaa ctttagagct gggctacgcc ttgcaggctc cgacgcagga
      901 gccggagccg gcgaaagtga cacgcgcggc gcgggccgcg gggctgctgc ggccttggct
      961 ttgggggtgg cggccgacga gggctttgtc gcgacctcgt cgggagcgag ctcggacgct
     1021 ggcgggctcg tcgtgcgacc aaacccgagg tccgcagtgg ccgcgttcag ttctcgggtc
     1081 tgctccgggc tggtccgagg ttgcggccgc ggcgcgggct ggaaagcgaa tgtgttgggc
     1141 ttgcttgtca tggcgtgtcc cctcccctgc tacgctgcgg cctgggcgtg agcggccaag
     1201 gcctcaagga tctcgccgag cacggcggtg atttctcggg cggctttggc gtctccgcct
     1261 tcgtgtgccg gtgtcgcgcc ggtgaagtgg atggactgat aggctgcgcg ctggatgatt
     1321 tcggtcttga ggactggaat ggagttggcc tgaatttctg accgggcgtg ctgcgccaca
     1381 cggctctgca ggggcgacat ctgggtcaga accacgcgcg ccggaatggg tcgtttcagc
     1441 atcttctccg cacgcttgac cacctcatag gtgcggaaca cctcctgcag gtccataccg
     1501 gagatcttgg tggggatcag cacgaggtcg ctttcagcga tggcgtaagt caccgcctgc
     1561 gacgcggcgc cttccagatc gacgacgacc agggcgtact tagccgccgc ttgagcgatg
     1621 acgtcctgga tcttggtttc gtcggcctcc gagatcaccg tgaccttgca ctcatcacct
     1681 gcccgctcgc gccaggtggc gacgtgaccg ttgggatccc catcgacgat ggccgtcgat
     1741 ccgccctggc gcgcgaaggc gtcggcgagg cacatgacca aggtagtctt cccgaccccg
     1801 cccttggtcg aacagacgct gatcaccgac atggccgtct ccctcaaatg accaactggg
     1861 caaatgcccc tttgcccgca cgggtttaag cccgcttggt aaagatccct ttaacccgag
     1921 cgccccctta ccctgttgcc ctattggggt tttgggtaga tgggcattca cccgtgcggg
     1981 caagagagta aaaccccttt ggcccatttg ggcaagaggg caacgcccca ttagggcaac
     2041 ggggtttttg ggtgtttgcc gtcgatctgt atcggccatg gctggggagc acccatcggg
     2101 ccacaacatc ggcgcccagg gttccccacc tatcacgact cggcaggggc gccgctgcat
     2161 ggcagagccc gaagacgacg cttgctgaag cacaccgtcg cccccgcacc ccatgcccat
     2221 cagatctgcg ctgcgctgcg agaggggagg gggcctcttg tctgacgcca aacctcagat
     2281 gacggcgcgg cggcgacgtt ttgacgtcgt cggccggcgc gccgggcgct tacagagtta
     2341 gattctatag atttatagca gttagagggg ccgtattgcg cttcccattc aatgacttag
     2401 ggccataatc tgtcctcgat atcacgagct tccgtccgcg atgtcccagg accgaggtcc
     2461 gcgatgtccc gacccttcgt ccgcgaagtc acgcctaagc gcctgttttt caaagactgt
     2521 ccgcgatatc ccgaagttcc gtccgccaag tcccgtgtgg gtgtccgcga tatcccgatc
     2581 cggccaggcg ccatttcaga ccaaatccag aacaaacggc gaaacgcctc gggatatcgc
     2641 ggacattggc ccgaaaagag tcgtgagatc gcggacgcgg gacctgccaa agctcgtgag
     2701 atcacggacg ccgcgaatcg gctcgggata tcgaggacaa agcgcaaagc tcgtgacatc
     2761 gcggacgcaa accccaagaa cgccctcgcg cgcgaggggc gctccagcac cgcagactgc
     2821 tgcagagtgg ggcgcggccc cacgaatggc gcggaaggcc ctctgccccc agccggagga
     2881 ggcctggccg ccctcggccg acccaggcga ccgcggcgaa ggggcgccaa ggctacgccg
     2941 ggcaggctca cccaggaaaa cgtcgatgca gggttagacg gccggggcgc ggcagcgatc
     3001 gagggaagcg caggggccca actcgtgggc gtgatatcgc ggactttagc cggcgagagc
     3061 gcggcgcggc ctgaccacga gataggcgcg cttggaaacc cgctgtggcc cgacgagctt
     3121 caggcctcgc ggctccttgg tgatttccag gt
//
LOCUS       DAOMEO010000272         3143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3463_length_3143_cov_2.288212, whole genome shotgun sequence.
ACCESSION   DAOMEO010000272 DAOMEO010000000
VERSION     DAOMEO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3463_length_3143_cov_2.288212"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>942
                     /locus_tag="PLH31_07335"
     CDS             <1..>942
                     /locus_tag="PLH31_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89065.1"
                     /translation="TPLDALPDLSDVQVVIRTSYPGQAPQIVENQVTYPLATTMLSVP
                     GAKTVRGYSFFGDSFVYVLFEDGTDLYWARSRVLEYLNQAQGRLPAAAKPALGPDATG
                     VGWIYEYALVDRSGGHDLSQLRSLQDWFLRYELKSLPGVAEVASIGGMVRQYQVVLDP
                     MKLAAYGVTHSQVVEAIKGANQETGGSVLELGEAEYMVRANGYLSSLDDFRAVPIRTA
                     TGGVPVRLADVANIQVGPEMRRGIAELNGQGEVAGGVVILRSGKNARETIAAVKTKLA
                     ELKRSLPPGVEVVTTYDRSQLIDRAIANLSQKLVEEF"
     assembly_gap    943..952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <953..3055
                     /locus_tag="PLH31_07340"
     CDS             <953..3055
                     /locus_tag="PLH31_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQR89066.1"
                     /translation="VVCGLFLWHGRSALVAILTLPLGVLAAFLIMRVQGLNANIMSLG
                     GIAIAIGAMVDAAVVMIENAHKHLERWAHDHPGEDLKGDRRWTVITEAAAEVGPALFF
                     SLVIIALSFVPVFTLQAQEGRLFAPLAFTKTYAMAAAAILSVTLVPVLMGYLIRGRIP
                     AEQDNPVNRALTGAYRPALDWVMRKPKTTLLLALLVFATTAWPLSRLGGEFLPPMDEG
                     DLLYMPSALPGLSAAKASALLQQTDRMILTVPEVKSVFGKAGRAETATDPAPLEMFET
                     TIQFKPRDQWRPGMTPEKLVDELDRAVKVPGLSNIWVPPIRNRIDMLATGVKSPIGVK
                     VSGSDLAELDRIAGRIEQVAKGVPGVSSALAERLTGGRYVDVDIDRVQAARYGLNIAD
                     VQSVVSGSIGGETVGETVEGLARYPINVRYPRELRDSLEGLRALPVVTPSGQQITLGT
                     VAAVRIADGPPMLKTENGRPSTFVYVDVRGRDLASVVHDLQAAVARDVKLSPGVSVAY
                     SGQFEYLQRAAERLKIVVPATLAIIFVLLFLTFGRLDEAALIMATLPFALTGGIWTLY
                     LLGYHQSVATGVGFIALAGVSAEFGVVMLIYLKHALADRGPDPTEPQIEAAVREGALL
                     RVRPKAMTVAVILAGLFPILIGHGAGSEVMSRIAAPMIGGMLTAPLLSMLVVPAGYLL
                     LRRRRLSTPASTQKGDIQ"
BASE COUNT          488 a         1080 c         1039 g          526 t
ORIGIN      
        1 aacaccactg gacgccctac ccgatctttc cgacgtgcag gtggtgatcc gcacctccta
       61 tcccggtcag gcgccgcaaa tcgtcgagaa ccaggtcacc tatcccttgg ccaccaccat
      121 gctctcggtc cccggcgcca agaccgtgcg cggctattcc ttcttcggcg acagcttcgt
      181 ctatgtgctg ttcgaggacg ggaccgacct ctattgggcc cgctcgcgcg tactggaata
      241 tctcaaccag gcgcagggcc gcctgcccgc cgccgccaag ccggcgctgg ggccagacgc
      301 cacgggcgtc ggctggattt acgaatacgc cctggtcgac cgaagcggcg ggcacgacct
      361 gtcgcagctg cgcagcctgc aggactggtt cctacgttat gagctgaaga gccttcccgg
      421 cgtcgcggaa gtggccagca tcggcggcat ggttcgtcag taccaagtgg tgctcgaccc
      481 gatgaagctc gccgcctacg gggtcaccca cagtcaggtc gtcgaagcga tcaagggcgc
      541 caaccaggaa accggcggct cggtgctgga gctgggcgag gccgaataca tggtccgcgc
      601 caatggctac ctctccagcc tcgacgactt ccgcgccgtg ccaatccgca ccgccaccgg
      661 cggcgtgccg gtgcggctgg ccgacgtcgc caacattcag gtgggcccag aaatgcggcg
      721 cggcatcgcc gaactgaacg gccagggcga ggtggccggc ggggtggtga tcctgcgctc
      781 gggcaagaac gcccgtgaga ccatcgcggc ggtgaagacc aagctcgccg agctgaagcg
      841 cagcctgccg cccggcgtcg aggtggtgac cacctacgac cgctcgcaat tgatcgaccg
      901 ggccatcgcc aacctcagcc agaagctggt cgaggagttc atnnnnnnnn nngtggtctg
      961 cggcctgttc ctctggcacg gccgttcggc cttggtggcc atcctcacct tgccgctcgg
     1021 ggtgctggcc gccttcctga tcatgcgagt ccagggcctg aacgcgaaca tcatgtcgct
     1081 cggcggcatc gccatcgcca tcggcgcgat ggtcgatgcg gcggtggtga tgatcgagaa
     1141 cgcccacaaa catctggagc gatgggcgca cgaccatccc ggcgaggacc tcaagggcga
     1201 ccggcgctgg acggtaatca ccgaggcggc ggccgaggtc gggccggcgc tgttcttcag
     1261 cctggtgatc atcgccctgt cgttcgttcc ggtgttcacc ctgcaggctc aggaagggcg
     1321 gctgttcgcg ccgctggcct tcaccaagac ctacgccatg gcggcggccg ccatcctgtc
     1381 ggtgaccctg gtcccggtgc tgatgggcta cttgatccgc ggtcgcatcc cggccgagca
     1441 ggacaacccg gtcaatcgag cgctgacggg cgcctaccgg ccggcgctgg attgggtgat
     1501 gcgcaaaccc aagacgaccc tgttgctggc gctactggtc ttcgccacca cggcctggcc
     1561 cctcagccgt ctgggcggcg agttcctgcc gccaatggac gagggcgacc tgctctacat
     1621 gccctcggcg ctgccgggac tgtcggcggc caaggcctcg gccttgctgc agcagaccga
     1681 ccggatgatc ctgacggtgc cggaggtgaa gagcgtgttc ggcaaggccg gccgggcgga
     1741 gaccgctacc gacccggcgc cgctggagat gtttgagacc accatccagt tcaagccccg
     1801 cgaccagtgg cggcccggca tgacgccgga gaagctggtc gatgagcttg accgggcggt
     1861 gaaggtcccg ggcttgtcca acatctgggt gccgccgatc cgcaaccgga tcgacatgct
     1921 ggcgaccggg gtgaagagcc cgatcggggt gaaggtctca gggtccgacc tcgccgaact
     1981 cgaccgcatc gccgggcgca tcgagcaggt ggccaagggc gtgccagggg tcagttcggc
     2041 cctggccgag cggctgaccg gcggtcgcta cgtcgacgtc gacatcgacc gtgtccaggc
     2101 cgcccgctac gggctgaaca tcgccgacgt gcagtcggtg gtgtccggtt cgatcggcgg
     2161 cgagaccgtc ggcgaaacgg tggagggcct ggcccgctat ccgatcaatg tccgctaccc
     2221 gcgcgaactg cgtgacagcc tggagggcct gcgcgccttg cccgtggtca ccccttcggg
     2281 ccagcagatc accttgggca cggtggcagc cgtgcggatc gccgatggcc cgccgatgct
     2341 gaagaccgag aacggccggc cctcgacgtt cgtctatgtc gatgtccgtg gtcgcgacct
     2401 cgcctcggtg gttcatgacc tgcaggccgc cgtcgctcgg gacgtcaagc tctcgcccgg
     2461 ggtcagcgtc gcctactccg gccagttcga atatctgcag cgggcggccg agcggctgaa
     2521 gatcgtggtg ccggcgaccc tggccatcat cttcgtcctg ctgttcctga ccttcggccg
     2581 cctcgacgag gcggctctga tcatggccac cctgcccttc gcgcttaccg gcggcatctg
     2641 gaccctctac ctgctcggct accaccagtc ggtggcgacg ggcgtggggt tcatcgccct
     2701 ggccggcgtc tcggccgaat tcggggtggt gatgctgatc tacctcaagc acgcgctggc
     2761 tgaccgtggc ccagacccca ccgaaccaca gatcgaggcg gcggtgcgtg aaggcgccct
     2821 gctgcgcgtg cggcccaagg cgatgaccgt ggcggtcatc ctggcgggcc tcttccccat
     2881 cctgatcggc catggggccg gttcggaagt catgagccgg atcgccgcgc ccatgatcgg
     2941 cggcatgctc accgcgccgc tgctgtccat gctcgtcgtc ccggcgggat accttctgct
     3001 gcggcgacgg cgtctttcca cccctgcttc aactcaaaaa ggagacatcc aatgaagcgc
     3061 accctgacca ccctcgccct tgtcggcgcc gctgcagctc tggctgcttg cggtccgaaa
     3121 accgagacca agaccgccga gcc
//
LOCUS       DAOMEO010000273         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3467_length_3140_cov_3.405511, whole genome shotgun sequence.
ACCESSION   DAOMEO010000273 DAOMEO010000000
VERSION     DAOMEO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_3140_cov_3.405511"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..345
                     /locus_tag="PLH31_07345"
     CDS             88..345
                     /locus_tag="PLH31_07345"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HQR89067.1"
                     /translation="MSPATILARAADVLADRSNTYGEPRQNMEDIAARWSVTLGHPVT
                     PAQVVLCMLDLKLARLRRDPGHQDSMVDVIGYAALLNEVTR"
     gene            535..987
                     /locus_tag="PLH31_07350"
     CDS             535..987
                     /locus_tag="PLH31_07350"
                     /inference="COORDINATES: protein motif:HMM:NF040389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6362 family protein"
                     /protein_id="HQR89068.1"
                     /translation="MADWTPGMVEERLIEAAAVLRRLPAQRGQGYFSTWPQMFVEFSD
                     LVGQTPDPMRLPPPSAAAISRMEATLGWMPWLEPIDAKIAWLRASGTRWKEICWEVGL
                     ARAAAHEHWLYALCVIAWRLNGRERVKHIGRRRLIARFRVGAVSGHPA"
     gene            1178..1615
                     /locus_tag="PLH31_07355"
     CDS             1178..1615
                     /locus_tag="PLH31_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR89069.1"
                     /translation="MFRHILIPTDGSLLSDAALDSGLRLARDCGARVTVVAVTEPFVV
                     LSAEARKIGVSQADHEREVKAAAAGHLTEAARKAGTLGVPCETVEVENEYPYRGIIET
                     AADRGCDLITMASHARRGISALVISSEAVNVMTHSGIPLLVYR"
     assembly_gap    1663..1672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1677..2381)
                     /locus_tag="PLH31_07360"
     CDS             complement(1677..2381)
                     /locus_tag="PLH31_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR89070.1"
                     /translation="MFLGAILYTSVGHAGASAYIALMALFGVPPATMRPTALTLNILV
                     ATLTSGRYIHAGAYRWRTLWPFLIGALPMAFIGGALQLPGHIYRPLVGTVLLLSAARL
                     LWPTELKAATQWRDPPIIGGILAGAAIGMLSGLTGTGGGIFLSPLLLFLGWSEPRSAS
                     GVAAVFILCNSVAGLLGNLTILQHLPPDLPLFAGATLAGALVGTTLGIRLAIPGILKA
                     LGLVLAIAGLKLIGVY"
     gene            complement(2675..2965)
                     /locus_tag="PLH31_07365"
     CDS             complement(2675..2965)
                     /locus_tag="PLH31_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HQR89071.1"
                     /translation="MSKTTRKQTTTKTAKRTTTKAAPAEAKALRTASKQARLIEMLRQ
                     PEGVTIDEIVKTLDWQAHTVRGAISGALKKKLGLTIESEKIDGRGRVYRVAE"
BASE COUNT          566 a          970 c         1073 g          521 t
ORIGIN      
        1 cgagcgcggc tacagcccgg ccgacgacaa cgaggccgat gccatcgcga tcctgctgtg
       61 ggcccttgag actcgaggag gcgtgcgatg agtccggcga ccatcctcgc gcgcgcggcc
      121 gacgtcttgg ctgaccgcag caatacctac ggcgagccgc gccagaacat ggaggacatt
      181 gccgcccgct ggtctgtcac gctcggtcac ccggtcaccc cggcccaggt cgtgctgtgc
      241 atgctggacc tcaagctggc ccggctgcgc cgcgatcccg gccaccagga cagcatggtc
      301 gacgtgatcg gctacgccgc gctgctgaac gaggtgacgc gatgaggtgg gcaccacgag
      361 gcttcggcgg cgagcgccag ccgcccgagg acatcaagcg acagggttgg caggcgcagc
      421 gcgtgctggt cgtcgatgcc gacgacgaac gtctgacctg gcccgagcgc gagctgatcc
      481 gccagcttgg cgagaagctc tacggcaaca ggccgaagcg acaggagacg cgacatggct
      541 gactggacgc cggggatggt cgaggagcgg ctgattgagg cggctgcagt cctgcggcgg
      601 cttcccgcgc aacggggtca gggctacttc agcacgtggc cccagatgtt cgttgagttt
      661 agtgacctcg tgggccagac gccggatccg atgcgcctgc cgccgccgtc ggccgctgcc
      721 atcagccgga tggaggcgac gctcggctgg atgccctggc tcgagccgat cgacgcgaag
      781 atcgcgtggc ttcgggctag tgggacgcgg tggaaggaga tctgctggga agtcggcctg
      841 gcccgcgccg ccgcgcacga gcactggctc tacgcgctct gcgtcatcgc gtggcggctg
      901 aacggcaggg aacgggtaaa gcacatcggc aggcggcggc tgattgcgcg gtttcgcgtt
      961 ggggccgtaa gcggccatcc tgcgtagtgg gtcggacttc cgaactgccg agtgccagag
     1021 gaaatgatcg ctcggtcaag ctgcgcgaag tcgcgcgaaa cagcggcctg tcccgaagtc
     1081 gccagacagc gtacacatca catgattgcg atcgggcttt gctcggcgcc aggtcgcgcg
     1141 gcgagagccg gcctctctcg gtctttgaaa gggccccatg ttccggcaca tcctaattcc
     1201 caccgacggc tcgttgctgt cggacgccgc cctcgacagc ggcctacgcc ttgcgcggga
     1261 ctgtggcgcc cgcgtcaccg tggtagcagt gaccgagccg ttcgtcgttc tgtcggccga
     1321 agcgcgcaag atcggggtct cgcaagctga ccatgaacgc gaggtcaagg cggcagcagc
     1381 gggacatctc accgaggcgg cgcgcaaggc ggggacccta ggcgtgccgt gcgagacggt
     1441 cgaggtcgag aacgagtatc cgtaccgtgg catcatcgag accgcggccg atcgaggatg
     1501 tgatctcatt actatggcct cgcatgccag gcgcgggatc agtgcgctgg tgatcagcag
     1561 cgaggccgta aacgtaatga cccacagtgg cattcccttg ctggtctatc gctgaggcgt
     1621 ctccctctca gcatcgcctc tcgttacccg aggggctgtg ccnnnnnnnn nngcactcaa
     1681 tagacgccga tcagcttcag acccgcgatc gccagaacaa ggcccagagc tttcaggatc
     1741 ccagggattg caagacggat tccgagggtg gtccctacca gcgcaccagc cagcgtcgcg
     1801 ccagcgaaca gtggcaagtc aggtggcaga tgctgcagga tcgtgaggtt gccgagcagg
     1861 ccggcaacgg aattgcacaa aatgaacacg gcagccacgc cggacgctga gcgcggttcc
     1921 gaccatccga gaaagagcag gagcggcgac aggaaaatgc caccgccggt gcccgtcagg
     1981 cctgacagca tgccaatcgc tgctccggcg agaatccctc cgatgatggg aggatcccgc
     2041 cattgggtgg ctgccttcag ttccgtcggc cagaggagac gggccgcgga gagcagcaga
     2101 acagtgccca cgagtggacg atagatgtgc ccgggtagct gcaatgcccc gccgataaac
     2161 gccatcggca gggctccgat caggaacggc catagggtac gccaccggta cgcgccggcg
     2221 tgaatgtacc ggcccgatgt caaggtcgcg acgaggatgt tcagcgtcag cgcggtcgga
     2281 cgcatggtag caggcggaac gccgaacagc gccatcagcg cgatgtaggc agatgctcct
     2341 gcatggccca cggacgtgta aaggatcgcc cctagaaaca tgcagagcgc cagaagccat
     2401 tgagcctcga tcaaaggggg ggcactcgct atattcattc cgctatgacg ataggggaga
     2461 acgaagcggt gctcaatgtt gcgtaaacta gccactcgga gcgccgggcc gaaaacgtcg
     2521 cttgcagaca cttttcgctc agacagattc agtcggatcg ggtagttttc aagccaagct
     2581 cgcgggacgt gtgcgcagcg ggcatcacca acaacacgaa cgacaaaccg gcgactacgc
     2641 cgtgacgcgg ccgccggttc ggtgatcgct cggtttactc ggcgacgcgg tagacgcggc
     2701 cgcgtccatc gatcttctcg gactcgatcg tgagcccgag cttcttcttc agagcgccag
     2761 agatggcgcc gcgcaccgtg tgggcctgcc agtcgagggt cttcacgatc tcgtcgatag
     2821 tgacgccctc tggctggcgt agcatctcga tcaagcgggc ctgcttgctg gcggtcctta
     2881 gagccttggc ttcggcgggc gcggccttcg ttgtggtgcg cttggcggtc ttggtggtgg
     2941 tctgcttgcg cgtggtcttg gacatggccg ttgctcctga tagcgagccg cgaccgtcgc
     3001 ggctcctact gaccagagcc ccgccagcct gagccggtcg gggctgggga gcgtggtcag
     3061 ctgctactcg tcgtgctcgc cttccctgaa ggcgctgtcg gtgatgcgct tcagcagttc
     3121 ggcgtagtgg gcgagggtgc
//
LOCUS       DAOMEO010000274         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3469_length_3139_cov_2.595655, whole genome shotgun sequence.
ACCESSION   DAOMEO010000274 DAOMEO010000000
VERSION     DAOMEO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_3139_cov_2.595655"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLH31_07370"
     CDS             complement(<1..682)
                     /locus_tag="PLH31_07370"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HQR89072.1"
                     /translation="MDAFSIDDVLAEVAVLVKPHFGKGRPADYIPELADVPGSRFGMA
                     VCTLDGGEHVIGDADEGFSVQSITKILALGLALNRFGDEVWTRVGKEPSGTPFNHLSQ
                     LEAEAGVPRNPFINAGALAITDQLLTTVKDPAALVRGFAGFLAGQPVEIDEAVAASEL
                     ATAHQNRAIANLMRGKGTITHDPEAVVAAYCRQCALSMSCRQLARAFLPLAAGGFSPV
                     IQETIFPDR"
     gene            complement(702..1865)
                     /gene="dapE"
                     /locus_tag="PLH31_07375"
     CDS             complement(702..1865)
                     /gene="dapE"
                     /locus_tag="PLH31_07375"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HQR89073.1"
                     /translation="MTSPAPASVSIDPVELAQALIRRPSVTPADEGAMDLLQRQLDAL
                     GFACRRMKFGEIENLYARRGTARPNLCFAGHTDVVPIGDGAAWTASPFEAEIRDGVLY
                     GRGAVDMKSAIAAFVSAVGNMPDTPGSISFLITGDEEGVAEDGTVRVVQALQAQGEII
                     DHCIVGEPTSASVLGDMVKIGRRGSINAWITVEGQQGHVAYPHRAANPIPVLVSILSR
                     LQSRVLDEGYEGFQPSNLEVTTIDVGNTATNVIPATARARVNIRFNPAHQGKDLQAWI
                     ETECAAAAEGFKARAEVMCKISGEAFLTKPGPFTDVIVAAVGEATGRVPELSTTGGTS
                     DARFIRSLCPVVEFGLVGATMHQVDERVPVQEIRYLADAYAALIRRYFEAFAA"
     gene            1930..2409
                     /locus_tag="PLH31_07380"
     CDS             1930..2409
                     /locus_tag="PLH31_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR89074.1"
                     /translation="MPKIDIDAAPTRFGTAYPPPFDAPCQGRQRWKLGDAAGLTQFGV
                     NLMRLPPGQWSSQRHWHTAEDEFTWVIEGEVVLVDDDGETVLGAGDCAGFKAGATNGH
                     HLQNRSDTVAVLLEIGSRRPQDDGCDYPDIDLILREGEETYRHRDGAPYAVEGGRKR"
     gene            2726..2818
                     /locus_tag="PLH31_07385"
     CDS             2726..2818
                     /locus_tag="PLH31_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89075.1"
                     /translation="MSRRDDRRSVQVAQGRRFASPRRGWPQASP"
BASE COUNT          492 a         1012 c         1115 g          520 t
ORIGIN      
        1 ggcggtctgg gaagatggtt tcctggatca ccggcgagaa gccgcccgcc gccaggggca
       61 ggaaggcgcg ggccagctgg cgacagctca tgctcagggc gcactggcgg caataggcgg
      121 cgaccacggc ctcgggatcg tgggtgatcg tgcccttgcc gcgcatcaga ttggcgatgg
      181 cgcggttctg gtgagcggtg gccagttccg aggcggcgac cgcctcgtcg atctcgaccg
      241 gctggccggc cagaaagccg gcaaagccgc gcaccagggc ggcggggtcc ttgaccgtgg
      301 tcagaagctg atcggtgatg gccagggcgc cggcgttgat gaacgggttg cgcgggacgc
      361 cggcctcggc ctccagctgc gagagatggt tgaacggcgt gcccgagggc tccttgccca
      421 cccgcgtcca gacctcgtcg ccgaaacggt tgagcgccag ccccagggcc aggatcttgg
      481 tgatgctctg gaccgaaaag ccctcgtcgg cgtcgccgat cacatgctcg ccgccgtcca
      541 gggtgcagac ggccatgccg aacctggagc ccggcacatc ggccagttcg gggatgtagt
      601 cggcgggccg gcccttgccg aagtggggct tcaccagcac ggccacctcg gccaggacgt
      661 cgtcgatcga gaacgcgtcc atggccggca tgctggaaat cctaagcggc gaaggcttcg
      721 aaatagcggc ggatcagggc ggcataggcg tcggcgagat agcggatctc ctgcaccgga
      781 acccgctcgt cgacctggtg catggtggcg ccgaccaggc caaattccac caccgggcac
      841 aggctgcgga tgaaccgcgc gtcgctggtg ccgcccgtgg tcgacagttc cggcacgcgg
      901 cccgtggcct cgccgacggc ggcgacgatc acgtcggtga acgggccggg ctttgtcagg
      961 aacgcctcgc cgctgatctt gcacatcacc tcggcgcgcg ccttgaagcc ctcggcggcg
     1021 gcggcgcact cggtctcgat ccaggcctgc aggtccttgc cctggtgggc cggattgaaa
     1081 cggatattga cgcgcgcccg cgccgtggcc gggatgacgt tggtggcggt gttgcccaca
     1141 tcgatcgtgg tgacttccag gttcgacggc tggaagccct catagccttc gtccagcacc
     1201 cggctttgca ggcgcgacag gatcgagacc agcaccggga tcgggttggc cgcccggtgc
     1261 gggtaggcca catggccctg ctggccttcc accgtgatcc aggcgttgat gctgccgcgc
     1321 cggccgatct tgaccatgtc gcccagcacg ctggcgctgg tgggctcgcc gacgatgcag
     1381 tggtcgatga tctcgccctg cgcctgcagg gcctgcacga ccctgaccgt gccgtcctcg
     1441 gccacgccct cttcgtcgcc ggtgatcagg aagctgatcg agccgggggt gtcgggcatg
     1501 tttccgaccg ctgagacgaa ggcggcgatg gcgctcttca tgtcgaccgc gccgcggcca
     1561 tagagcacgc cgtcccggat ttcggcttca aacgggcttg cggtccaggc cgcgccgtcg
     1621 ccgatgggca ccacgtcggt gtgaccggcg aagcagaggt tgggccgcgc cgttccgcgc
     1681 cgggcgtaga ggttctcgat ctcgccaaac ttcatccggc ggcaggcaaa gcccagggcg
     1741 tccagctggc gctgcagcag gtccattgcg ccctcgtcgg cgggggtcac cgaggggcgg
     1801 cggatcaggg cctgggcgag ctcgacaggg tcgatgctga cagatgcggg cgcggggctg
     1861 gtcatgatgc tggctttagg cttccctccc gccggacgca aacgctgcaa acctgttcca
     1921 cggatcccca tgcccaagat cgatatcgac gcggcgccca cccgtttcgg cacggcctat
     1981 ccgccgccct ttgacgcgcc ctgccagggg cgtcagcgct ggaaactggg cgatgcggcg
     2041 ggcctgaccc agtttggcgt caatctgatg cgcctgccgc cgggccagtg gtccagccag
     2101 cgccactggc acaccgccga ggacgagttc acctgggtga tcgagggcga ggtggtgctg
     2161 gtcgacgatg acggcgagac ggtcctgggg gccggcgact gcgcgggctt caaggccggc
     2221 gccaccaatg gccatcacct tcagaaccgc tcggatacgg tggcggtgct gctggagatc
     2281 ggctcgcgcc ggccgcagga cgatggctgc gactatcccg atatcgacct gatcctgcgc
     2341 gaaggcgagg agacctatcg gcatcgggac ggcgcgccgt acgcggtgga gggcgggcgc
     2401 aagcggtagg ggactgaagg ccggcgcgct ggcgccggcc gtgcggcgga cagatgggtg
     2461 ccgggaacga gtcccgggat ggcggggggc tatcgcatat gatccaaaac ggctgtcatc
     2521 ctggacgagc gcgcagcgcg aagatccggg accgacgcct gagcgcagcg ttccaggtcg
     2581 gtcccggatc ggcgcccggc ttcgccgagc ttgtccggga tgacaatttt tgccgcctgt
     2641 ttcacgtgcg attgccctgc gggatgacgg agggttgcgg ggtctgaagg cgaagccgcc
     2701 gcccctcggg cggagccatc cttacatctc acggcgagac gatcgccggt ctgtccaggt
     2761 tgctcaagga cgacgcttcg cgtcgccgcg gcgcggctgg ccgcaggcca gtccttgacc
     2821 aacctggaca gaccggcacg ctgcagcctc caagagatgt aaggcttggc gcgacgcgag
     2881 ggcgtcgggt tacggcggga ttcagatgga cgccttcacg ccaaagcgtg ctgatgcccg
     2941 caagcccgac cgtaaagcgc tcatctttcc tcgaagcctt gggcatttgg ctagagacgg
     3001 ctgggttccg aagttcacgc ccttcgtccg attctgacgg agctaaactt gattgatagc
     3061 ttttagacac gcatagattt cgccggtgat cctccctcgt ttcagtggac acccatgcta
     3121 gcttttcgga gctggagag
//
LOCUS       DAOMEO010000275         3137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3472_length_3137_cov_2.391304, whole genome shotgun sequence.
ACCESSION   DAOMEO010000275 DAOMEO010000000
VERSION     DAOMEO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_3137_cov_2.391304"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..2186)
                     /locus_tag="PLH31_07390"
     CDS             complement(399..2186)
                     /locus_tag="PLH31_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR89076.1"
                     /translation="MPHEVSPESYKDLVLFLATAGIVAPLFKRLKLNPILGFLLAGVI
                     LGPFGLGRFIPVAPWLDYVTVDNPDEIAQLAEFGVVFLLFMIGLELSWERLRLMRKLV
                     FGLGAAQVIGCSIALGLGAMALGQPPVAALAIGAALTLSSTAIAVPVLAERRRLHSDG
                     GRATFSVLLFQDLAVAPILITLTILGRGDGAFALKDLAALAPAALGLAGLVLIGRLVL
                     RPMLKSVAKAKSEEMFMAACLLVIIGAGMVAALSGLSMALGAFVAGVLLAETEYRHEV
                     EVKIEPFKGLLLSLFFVSLGIRLDLSLLFANPVLILGVGLGLLLVKGVIVFLGGMAMK
                     LGVKASTEAALILAAGGEFAFVILDNAMGAQVVPRDIGQAVLVSATLTMFLIPALAGL
                     GARFGRTSNTQISEAPKPAVERLDPEPEGQVLVVGYGRVGKLVADMLNRHDLPWTAVE
                     RDARLVEQGRRDGSRIYYGDASRLELLERCGLATARAVVITMDAPEAAEAVVAAVRGA
                     RPDISIVVRARDARHAARLYELGATDAVPETIEASLQLSEAVLVDIGVPMGLVIASIH
                     ERRDEYRKILNRPDALGGKRRRLRDASLR"
     gene            complement(2206..2454)
                     /locus_tag="PLH31_07395"
     CDS             complement(2206..2454)
                     /locus_tag="PLH31_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89077.1"
                     /translation="MPHAKILLPAQCRGARGLLSWSQGELADRAGVSRSTVKDFETER
                     HALHHSTERLLIEALEAGGVALIPADEAGAGVRLKMAS"
     gene            2526..>3137
                     /locus_tag="PLH31_07400"
     CDS             2526..>3137
                     /locus_tag="PLH31_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR89078.1"
                     /translation="MHHTSLIATIVAGLGLAFVFGALANRLKLPVLVGYLLAGVMVGL
                     HFSLKDLMAVRKIAIPGAVVQITAATLMGLGLALALGWTVGAGIVFGLALSVASTVVL
                     LRALQERRLVETGRGKIAVGWLIVEDLAMVLALVLLPALSGMLGGEAAAPGPGGVAGA
                     FAITIGKVVAFVAFMLIVGRRVIPWILHRIAHTGSRELFRLGVL"
BASE COUNT          540 a         1058 c         1032 g          507 t
ORIGIN      
        1 cgccaggagt ttgaatttca tatcaagagc cctctattgc cctgctccgg ccgaaaacga
       61 ccgagcatcg ttataccaag gcgaacgtcc cgggaaagtg tcagcaaagc cacactcggc
      121 aggtttcgct gacctggata tctgctccag gatgccaaac tagaccgccc cacaaaatca
      181 acttccgagc gtatcggctg aaatcttccg ctgagagcgc cagcttgttg gttcgcacgt
      241 gaaactcggt cgcgcaaggg cttgctgcat gaaaaggcgg cgctttgtcg cagctcggcc
      301 acatccgggc cgttcttcgc gcgtcagggg aagattaccc gctgaatcca gcgccgacgg
      361 atgatccgta tccggccgtc ggcagagctg agcgccggtt agcgcaacga cgcgtcccgc
      421 agccgccgcc gcttgccgcc gagggcgtct ggccggttca ggatcttgcg atactcgtcg
      481 cggcgctcgt ggatggaggc gatcaccagg cccatcggca ctccgatatc taccagcacg
      541 gcctcggaca gctgcaggct ggcctcgatg gtttcaggga cggcgtcggt ggcgcccagt
      601 tcgtacagtc gcgcggcgtg gcgagcatcg cgggctcgca ccacaatcga gatgtccggc
      661 cgcgcgcctc gtacggccgc cacaaccgct tcagcggctt ccggtgcgtc catcgtgatg
      721 accacggcgc gggcggtggc caggccgcac cgttcaagca actctagccg cgaggcgtcg
      781 ccataataga tgcggctgcc gtcgcggcgg ccctgctcga ccagacgcgc gtcgcgctcg
      841 acggcggtcc agggcaggtc gtggcgattg agcatgtcgg ccaccagctt gccgacccga
      901 ccgtagccga ccaccagaac ctgaccctcc ggttcggggt ccagacgttc gaccgcgggt
      961 ttcggggcct cggagatctg ggtgttggat gttcggccaa accgcgcgcc cagccccgcc
     1021 agggccggaa tcaggaacat ggtcagggtc gccgaaacca gtactgcctg gccgatgtcg
     1081 cgcggcacca cctgcgcgcc catggcgttg tcgagaatga cgaaggcgaa ctcgccgccg
     1141 gccgccagga tcagcgccgc ctcggtcgag gccttgacgc ccagcttcat ggccatcccg
     1201 cccaggaaga cgatcacgcc cttcaccagc agcaggccaa gaccgacgcc caggatcagc
     1261 accggattgg caaatagcag agacaggtcc aggcgtatgc ccagcgacac gaagaacagg
     1321 ctcagcagca ggcccttgaa gggctcgatc ttgacctcga cctcgtggcg atactcggtc
     1381 tcggccagca gcactccggc tacaaacgcg cccagggcca tcgacaggcc cgacagggcg
     1441 gccaccatgc cggcgccgat gatcaccagc aggcaggccg ccatgaacat ctcttcgctc
     1501 ttggccttgg cgacggattt cagcatcggc cgcagcacca gtcgaccgat caagaccagg
     1561 ccggccaaac cgagggcggc aggcgccagg gcggccaggt ctttcagggc gaacgctccg
     1621 tcgcctctcc ccaggatcgt cagggtgatc aggatcgggg ccaccgccag gtcctggaac
     1681 agcagcaccg aaaaggtggc cctgccgccg tccgagtgca gccggcgccg ctcggccagc
     1741 accggtacgg cgatggcggt cgaagacagg gtcagggccg cgccaatggc cagggccgcg
     1801 acaggcggct ggcccagggc catggcgcca aggcccaggg cgatggagca gccgatgacc
     1861 tgggcggcgc cgaggccgaa gaccagcttg cgcatcaggc gcagacgctc ccacgacagc
     1921 tccagcccga tcatgaacag caggaagacg acgccgaact cggccagctg ggcgatttca
     1981 tcggggttgt cgacggtgac atagtcgagc cagggcgcga cggggataaa ccggcccagg
     2041 ccaaatggcc ccaggatcac gcccgccagc aggaagccga ggatgggatt gagcttgagg
     2101 cgcttgaaca gcggcgcgac gataccggcg gtcgccagga acaggacaag gtccttgtag
     2161 ctctccggtg aaacctcgtg cggcacgccc cgtccttcgc tctctctagc tcgccatttt
     2221 cagtctgacg cccgcgccgg cctcgtccgc cgggatcaac gccacgccac cggcttccag
     2281 cgcctcgatc aggagacgtt cggtgctatg gtgcaacgca tggcgctcgg tctcgaaatc
     2341 cttgaccgta ctgcgtgaca cgcccgcccg atcggcgagc tcgccctgcg accaactgag
     2401 caggccgcga gcgccccggc attgcgctgg gaggaggatt tttgcgtgtg gcatctggca
     2461 aatccggatg aaatcaccca tattggttga gtacgcccga aaacgttgac ggtcaaggag
     2521 agcctttgca ccacacctcg ctgatcgcca ccattgttgc gggcttgggg ctggccttcg
     2581 tgtttggcgc cctcgccaac cgcctgaagc tgcccgttct ggtcggctat ctgctggccg
     2641 gggtgatggt cgggctgcat ttctcgctga aagacctgat ggcggtgcgc aagatcgcca
     2701 tacccggcgc cgtggtgcag atcaccgccg ccaccctgat gggcctgggt ctggcgctgg
     2761 cgctgggctg gactgttggg gcgggcatcg tctttggcct ggccctgtcg gtggcctcca
     2821 ccgtggtgtt gctgcgcgcc ctgcaggagc ggcggctggt cgagaccggg cgcggcaaga
     2881 tcgccgtcgg ctggctgatc gtcgaggacc tggccatggt cctggcgctg gtcctgctgc
     2941 ccgccctgtc ggggatgctg ggcggagagg ccgccgcgcc cgggccgggc ggcgtcgccg
     3001 gcgccttcgc gatcaccatc ggcaaggtcg tggccttcgt cgccttcatg ctgatcgtcg
     3061 gtcgccgggt gatcccctgg atcctgcacc gcatcgccca taccggctcg cgcgagctgt
     3121 tccgcctggg cgtgctg
//
LOCUS       DAOMEO010000276         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3478_length_3132_cov_2.357491, whole genome shotgun sequence.
ACCESSION   DAOMEO010000276 DAOMEO010000000
VERSION     DAOMEO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3478_length_3132_cov_2.357491"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..614
                     /locus_tag="PLH31_07405"
     CDS             213..614
                     /locus_tag="PLH31_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HQR89079.1"
                     /translation="MQWTSRLAPSLDEFAALAEAAFKALPEPFASLSADVEIRIEDFA
                     AEDVLDGLGIEDPFELTGLYQGVDLGRRSVLDSGVQPSMVFLYRRPILDEWCERGDVS
                     LGELISHVLVHEIGHHFGLSDDDIHRIEDDA"
     gene            614..1027
                     /locus_tag="PLH31_07410"
     CDS             614..1027
                     /locus_tag="PLH31_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HQR89080.1"
                     /translation="MSKPQFDLDAFRDRMSEGSFANGLDMATDGAVALVALEDEIVTA
                     HVQGGELYVVELRPPAEGTCTCPGFDKFGACKHQVGVAAAVNALDIAGLQKAQGRMAR
                     LRDGLALESQEALIERLVELARRRPEVLAALEGGL"
     gene            1268..2011
                     /locus_tag="PLH31_07415"
     CDS             1268..2011
                     /locus_tag="PLH31_07415"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HQR89081.1"
                     /translation="MDTPEQPPPPPAPERGRPVLWLLFVAAIIGIVIAMARAFPEAVR
                     TPDDWSYVIYGLGMVVLVTSGLSRIRGRLQVMQHLRYAAIWAAIVAVLALGFAYADEL
                     EGVSNRVRIAFSGGAPVATAERELVIPRDETGSFVVAAQVNGQRVRFLVDTGATETVL
                     SPDDARRLGLDPDALRYDRQSETANGLGFGASHVVERLEIGAIRFDAFPMVVNKAPMS
                     TSLLGMSFLDRLESYEVRGRTLTLKWRAL"
     gene            2142..>3132
                     /locus_tag="PLH31_07420"
     CDS             2142..>3132
                     /locus_tag="PLH31_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR89082.1"
                     /translation="MLPGLMQTTPLMISGILTYAQQAHGAREIVSKLIDEPVWRYDYA
                     GLGRRSAQLAHALKRLGVAPGDRVTSLAWNTHRHLELFYAVPGIGAVLHTANPRLFDE
                     QIVYTINHAESAVLVFDRNFAALVERIAPQLTTVKTFVMLSDAERTIPGSVGAISYET
                     LIAGEPETVDWPSFDENAGAFLCYTSGTTGAPKGVLYSHRAVVLHAMAGGLNSAFGFT
                     AFDVVMPCSSLYHATAWGLPFSAPMCGTKLVLPADKMDGASLHALIEGEGVTFTGGVP
                     TIWTMYLDWLEKTGESPTSLKRVVIGGSAVPRAMAESFKRLYGVSVLQIWGMTE"
BASE COUNT          499 a         1069 c         1027 g          537 t
ORIGIN      
        1 cagcgtcgcg caaatggcga ggcagaccgc gccggccgcc gccgcgagga ttagttcatg
       61 agagttcatc aggccggtcg ctagagcccc aggcagttca cgaatcagaa tcacagatta
      121 gtcgggccat gaccagagcg cgatcagcat ggctgtcgca gggcctgtcg caggcgactg
      181 gcgaaactgg ccaatcggtc tatctgagag tcatgcaatg gacctctcgc ctcgccccgt
      241 cgctcgacga attcgccgcc ctcgccgagg ccgccttcaa ggccctgccc gagcccttcg
      301 ccagcctgtc ggccgatgtc gagatccgca tcgaggattt cgccgccgaa gacgtgctcg
      361 acggcctggg catcgaagac ccgttcgagc tgaccggcct ctatcagggc gtggatctgg
      421 gccgccgctc ggtgctcgac tccggcgtcc agccctcgat ggtgttcctg tatcgccgcc
      481 cgatcctcga cgaatggtgc gagcgcggcg acgtctcgct gggcgagctg atcagccatg
      541 tgctggtcca cgagatcggc caccattttg gcctgtccga cgacgacatc caccgcatcg
      601 aggacgacgc ctgatgagca agccccagtt cgatctcgac gccttccgcg accggatgag
      661 cgagggctcg ttcgccaacg gcctcgacat ggcaaccgac ggcgcggtcg ccctggtcgc
      721 cctcgaggac gagatcgtca ccgcccacgt ccagggcggc gagctttatg tggtcgagct
      781 gcgtccgccc gccgagggaa cctgcacctg cccgggcttc gacaagtttg gggcctgcaa
      841 gcaccaggtc ggcgtcgcgg cggcggtcaa tgcgctggac atcgccggcc tgcagaaggc
      901 gcaaggccgc atggcgcggc ttcgcgacgg cctggccctg gaaagccagg aagcgctgat
      961 cgagcggctg gtcgagctgg cgcggcggcg gccggaggtg ttggcggcgc tggagggcgg
     1021 gctttaattc atcgctctcc ctaaaaatct ccgccctccc cggcgacccc ggtttgcgct
     1081 ttccacaccg gcgatcgtct cgccgtgaga tgaaaggatg gcttccttct cgcgggcggg
     1141 aaggcgggcg gtgcgaccgc tttaaatttc tcagcgcgaa cactggggaa agcttgatca
     1201 atcgccgctg cgcgccaacg tacactgcac gcattcaatg atctgcgacg cactttgcgg
     1261 ggaaactatg gacacaccag agcaaccgcc gccaccacct gcccccgaac gcggccgccc
     1321 cgtcctctgg ctcctcttcg tcgccgccat catcggcatc gtcatcgcca tggcccgggc
     1381 ctttcccgag gcggtgcgca cgcccgatga ctggagctac gtgatctacg gcttgggcat
     1441 ggtggtcctg gtcacctctg gcctgtcccg gatccggggg cggctgcagg tcatgcagca
     1501 cctgcggtac gccgcgatct gggccgcgat cgtcgccgtg ctggccctgg gctttgccta
     1561 tgccgacgag ctggaaggcg tctcgaaccg cgtgcggatc gccttcagcg gcggcgcgcc
     1621 cgtggcgacg gccgagcgcg agctggtcat tccgcgtgac gagaccggat cgttcgtcgt
     1681 cgccgctcag gtcaacggcc agcgcgtgcg cttcctggtc gacaccggcg ccaccgagac
     1741 cgtgctcagc cccgacgacg cccgccgcct gggcctggac cccgacgccc tgcgctatga
     1801 ccgccagtcc gagaccgcca acggcctggg ctttggcgcg agccatgtgg tcgaacggct
     1861 ggagatcggc gcgatccgct tcgacgcctt tccgatggtg gtcaacaagg cgccgatgag
     1921 cacctcactg ctggggatga gctttctgga ccggctggaa tcgtacgagg ttcggggcag
     1981 gaccctgacg ttgaagtggc gggccctctg acatgttgga agggacggcc tgaagaccgg
     2041 cttcgggtgt tgggactggg gattgcggtc cgccaccctc ttccccagac cccgccccac
     2101 gccctaggct gatcccaacg acaataaagg gagcgccgcg catgttaccg ggcctgatgc
     2161 agaccacgcc gctgatgatc agcggcatcc tgacctatgc gcagcaggcc catggcgcgc
     2221 gggaaatcgt ctccaagctg atcgacgagc cggtctggcg ttacgactat gccgggcttg
     2281 ggcgccgctc ggcccagctg gcgcatgcgc tgaagcgcct gggcgtggcg cccggcgatc
     2341 gggtcacgtc cctggcttgg aacacccatc ggcatctgga gctgttctat gccgttcccg
     2401 gcatcggcgc ggtgctgcac accgccaatc cccgtctgtt cgacgagcag atcgtctaca
     2461 cgatcaatca cgcggaaagc gcggtgctgg tcttcgaccg caacttcgcg gccctggtcg
     2521 agcgcatcgc cccgcagctg accacggtga agacctttgt catgctgtcg gacgccgagc
     2581 ggaccatccc cggatcggtc ggcgcgatct cttacgagac cctgatcgcc ggcgaacccg
     2641 agacggtgga ctggcccagc ttcgacgaga acgccggggc cttcctctgc tacacctcag
     2701 gcacgacggg cgcccccaag ggcgtgctct attcgcaccg ggcggtcgtg ctgcacgcca
     2761 tggccggggg cctgaacagc gcctttggct ttaccgcctt cgacgtggtg atgccctgct
     2821 ccagcctcta tcacgccacc gcctggggcc tgccgttcag cgcgccgatg tgcgggacca
     2881 agctggtttt gcccgccgac aagatggacg gcgcgtccct gcacgccctg atcgagggcg
     2941 agggcgtcac cttcaccggc ggcgtgccga ccatctggac gatgtatctg gactggctgg
     3001 agaagaccgg cgagagccca accagcctca agcgcgtggt tatcggcggc agcgcggtgc
     3061 cgcgggccat ggccgagagc ttcaagcggc tctatggcgt gtcggttctg cagatctggg
     3121 gcatgaccga ga
//
LOCUS       DAOMEO010000277         3126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3481_length_3126_cov_2.959622, whole genome shotgun sequence.
ACCESSION   DAOMEO010000277 DAOMEO010000000
VERSION     DAOMEO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_3126_cov_2.959622"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..766
                     /gene="lptC"
                     /locus_tag="PLH31_07425"
     CDS             152..766
                     /gene="lptC"
                     /locus_tag="PLH31_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQR89083.1"
                     /translation="MTSFAPSSALVLRRKIRPRRRKSISALRLALPALALIVGGAIAT
                     QAVIRAARAKASITVTSAPLRMENPRFTGTMKDGREFLIVATAAIRDKVDPNKVALEA
                     PELTRGYGSDNPTRVVARQGNYDEAKGALLLTGRVEIENGDGYAFKTEKAVIDTRTGE
                     LMGDAGVEGAASGAQVKADSYSVTDKGDRVIFKGRVRSRLNPGN"
     gene            786..1949
                     /locus_tag="PLH31_07430"
     CDS             786..1949
                     /locus_tag="PLH31_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="HQR89084.1"
                     /translation="MTPAKTAISRPKVCFLYIAQTHQILHSLPIAVALAKGWPGLEVE
                     IAATTQDHLDYARQLLDALGGAPIASRLLPPAWLRRVRLKEDSTPPKALMLAANALAL
                     GRMDAIVTPERTTALLRKLGVRKSLLVYTQHGAGDRGGVFEPRLRQFDLVMAAGPKQR
                     ARMVDGGWVKPENCAMVGYPKFDIIDALPGSPLPSFPDAKPIVIYNPHFHATLGSWPR
                     FGVSVLEQFAANDRFNLIFAPHIRLFDGATPAMLESLAPFRDHPRIHIDLGGPAAIDM
                     TYTRAADIYLGDVSSQVYEFLRTPKPCLFLNPSNADWQDDESFHHWRYGPVLASTDRL
                     IDAVEAARMGHGDYLQAQKTGFAQSFDLTDTPSSQRAAQAIAERVLARVRQAG"
     gene            1946..>3126
                     /locus_tag="PLH31_07435"
     CDS             1946..>3126
                     /locus_tag="PLH31_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQR89085.1"
                     /translation="MSAERSILARVLANAGTLLGGRTVNAVVSLGYIALTARGLSPAQ
                     MGVLVLINAFAQFLGDVVKFQSWQTVLQYGAGPLLEGDRPRFQQVVRLSLFLDLVSGL
                     AGVTLGVGGAFLLGPVLGWSSQVAPIAALYALTIFVMTSATSVGLLRLFDKFRYLAGE
                     QAISSTVRLIGAAIALSTHAPLWAFLLAWALGPLVSFLYVGIVAWREMGRRNLLEGFR
                     LRGALSEGLPGVWKFAWATNFSSSLDVAFTHVITLMVGALLGPTQAALWRIGRQVADG
                     MAKPARLVIPALYPELAKMRAMGGDAAMRKLAGQIALLGGAVAGVLLLITTLFGKPLL
                     ILVMGKAYESAAGVMTWQVAAAAIGVLALPLEPMLVSMRAPGDALRVRMVVCAVFLLA
                     LT"
BASE COUNT          469 a         1095 c         1009 g          553 t
ORIGIN      
        1 cccgctatct gcaggtcagc gtccaggaca ccgtcacggg ctccgcgacc tctggcgctg
       61 cgagggcgca tggggacggg ctggaatatc tcttgggtgt tggcccggac gggattaagc
      121 cggacgtctg acctcgccca tcttggccgc catgacctcg tttgctccgt cttcagccct
      181 cgtcttgagg cgtaaaatcc gaccgcgccg gcgtaagtcg atcagcgcgc tgcgactggc
      241 cctgccggcc ctagccctga tcgtaggcgg cgcgatcgcg acccaggctg tcatccgcgc
      301 tgcccgggcc aaggcctcaa tcacggtgac cagcgcccca ttgcggatgg aaaacccgcg
      361 cttcacgggc acgatgaagg atggccgcga gttcctgatc gtcgccaccg ccgccattcg
      421 cgacaaggtc gaccccaaca aggtcgcgct ggaagccccc gagctcacgc gtggctacgg
      481 ttccgacaat ccaacccggg tcgtggccag acagggaaac tatgacgagg ccaagggcgc
      541 cctgctgctg acaggccgag tggagatcga gaatggcgac ggctacgcct tcaagaccga
      601 gaaggcggtc atcgacacgc gcaccggcga gctgatgggc gacgccggtg tcgagggcgc
      661 cgcctctggc gcccaggtca aggccgatag ctattccgtc acggacaagg gcgaccgggt
      721 gatcttcaag ggccgagtgc gctcgcgcct caatcccggc aattgaaccg gccggcgacg
      781 cgctcatgac cccggcaaag accgccatat ccagacccaa ggtctgtttt ctctacatcg
      841 cgcagacgca ccagatcctc cacagcctgc ccatcgccgt cgccctggcc aagggctggc
      901 cgggccttga ggtcgagatc gcggccacca cccaggatca tctcgactac gcacgccagt
      961 tgctggacgc gctgggcggc gcccccatcg cctcccggct gctgccgccg gcctggctgc
     1021 gcagggtgcg cctgaaggag gattcgaccc cgcccaaggc cctgatgctg gccgccaacg
     1081 ccctggccct gggccggatg gacgccatcg tcacgccgga acgcaccaca gccctgctgc
     1141 gcaagctggg cgttcgcaag tcgctgctgg tctataccca gcacggggcc ggcgatcgcg
     1201 gcggggtgtt cgagccacgt ctgcgccagt tcgatctggt gatggccgca ggacccaaac
     1261 agcgcgcccg catggtcgac gggggctggg tgaagcccga aaactgcgcc atggtcggct
     1321 atcccaagtt cgacatcatc gacgccctgc ccggctcgcc gctaccgagc tttcccgacg
     1381 caaagccaat cgtgatctac aacccgcact tccacgcgac cctgggctcg tggccgcgtt
     1441 ttggcgtcag cgtgctggag cagttcgcgg cgaatgatcg cttcaacctg atcttcgcgc
     1501 cgcacatccg gctgttcgac ggcgccactc cggccatgct ggagagcctg gccccgttcc
     1561 gcgaccatcc ccgcatccat atcgatttgg gcggccccgc cgccatcgac atgacctata
     1621 cccgcgccgc cgacatctat ctgggcgacg tctccagcca ggtctacgag ttcctgcgca
     1681 cgcccaagcc ctgcctgttc ctcaatccgt caaacgcgga ctggcaggac gacgagagct
     1741 tccaccactg gcgctatggc ccggtgctgg cgtcgaccga ccgcctgatc gacgctgtcg
     1801 aggcggcccg catgggtcat ggcgactatc tgcaggccca gaaaactggc ttcgcccaga
     1861 gctttgacct gaccgacacc ccgtcctcgc agcgcgcagc ccaggcgatc gccgagcgcg
     1921 tgttggccag ggtccgccag gccggatgag cgccgagcgc tccatcctgg cccgggtgct
     1981 ggccaacgct ggaaccctgc tgggtggccg gacggtcaac gccgttgtca gcctgggcta
     2041 tatcgccctg acggcccgag ggctcagccc ggcccagatg ggcgttctgg tgctgatcaa
     2101 cgccttcgcc cagttcctgg gcgacgtggt caagttccag tcctggcaga ccgtgctgca
     2161 atatggcgcc gggccgctgc tggagggcga tcgcccgcgc ttccagcagg tggtgcggct
     2221 gtcgctgttc ctggacctgg tcagcggact ggcaggggtt acgctcggcg tcggcggcgc
     2281 atttctgctt gggccggtgc tgggctggtc gtcccaggtc gcgcccatcg cggcgctcta
     2341 cgccctgacc atcttcgtga tgacgtcggc cacctcggtg ggcctgctgc gtctgttcga
     2401 caagttccgt tacctggccg gtgagcaggc catcagctcc acggttcggc tgatcggcgc
     2461 ggccatcgcc ttatcgaccc acgcccccct atgggctttc ctgctggcct gggccctcgg
     2521 ccccctggtc tcctttctct atgtcgggat cgtcgcctgg cgcgaaatgg gccgccggaa
     2581 cctgctggag ggttttcgcc tgcgcggcgc gttgagcgag ggtttgccgg gcgtctggaa
     2641 atttgcctgg gcgaccaatt tcagcagcag tctggacgtg gccttcaccc atgtgatcac
     2701 cctgatggtc ggcgccctgc tgggtcctac ccaggctgcg ctttggcgga tcggccggca
     2761 ggtggccgat ggcatggcca agcccgcccg gctggttatc cctgcgctct atcccgaact
     2821 ggccaagatg cgcgccatgg gcggcgacgc ggcaatgcgc aaactggccg gccagatcgc
     2881 cctgctgggc ggcgcggtgg ccggcgtgct gctgctgatc actaccctgt ttggcaaacc
     2941 gctcctgatc ctggtcatgg gcaaggctta tgaatcggcg gcaggcgtca tgacctggca
     3001 ggtggccgcc gccgcgattg gcgtgctggc cctgccgctc gaacccatgc tggtgtcgat
     3061 gcgcgcgccg ggcgacgccc tgcgtgtccg catggtggtc tgtgcggtct tccttctggc
     3121 gctgac
//
LOCUS       DAOMEO010000278         3123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3485_length_3123_cov_3.479465, whole genome shotgun sequence.
ACCESSION   DAOMEO010000278 DAOMEO010000000
VERSION     DAOMEO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3485_length_3123_cov_3.479465"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1720)
                     /locus_tag="PLH31_07440"
     CDS             complement(65..1720)
                     /locus_tag="PLH31_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HQR89086.1"
                     /translation="MTLSVKSRLLAAGGTVAALALFYGSSASAGTCETAAIQAIAPAD
                     TVITSATTTAAPVPHCRIEGYITTNNPGPNKVSFRLQLPDTGWQKRYYFIGMGGAAGY
                     VPSDSQIPAGNPLLKGFAVAGTDTGHTGHILDWSFLADPAKARDHIDRGAHVTAVATQ
                     QITRAYYDVESFFRYTSGCSGGGRMTTEAIERHPEDYDGVLLGAPGGRSSATMLAFIN
                     AAQQMAREPGSWVSPAKFVMLDAKITANCDELDGAKDDIIQDHTKCKVDFDAFACAKG
                     DGPDCLTQPEIKSIKAIVAGPRSPAGLIKVGFPVSNMSVWAGFMGMAPPPWSDAPTME
                     NMAKSSPGYVIGSSLAKVLFGPDFDVMKMNLNDQAVIDAWWAATKKLDFGWPYSANLA
                     SYHAKGGKVLMWNGVSDPCCIDTEMEQYYMDAATSVGGMKNLRQFASFYRIPGMGHCG
                     SGTGPGDGPDVLMTELINWVEKGEAPGPVVAHRGADRQQLLFADPATKMVSGVLVPPP
                     TGSSRDFILCRFPEVAVFDRSKAATPNAVYDAANWSCKAERRT"
     gene            complement(1822..>3123)
                     /locus_tag="PLH31_07445"
     CDS             complement(1822..>3123)
                     /locus_tag="PLH31_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89087.1"
                     /translation="KPMGQIWLWCSIASMLLEEDPDYFANFWTQAGYIGHDRPDVVKD
                     DLIDLTLPISRIITAQDLMGEEFAGPEYADSKAMILLMASMSGAWDLPVAIEVKGLKG
                     GYSTGCGVLIKSGGAAGRQLFCTRAVGDIWFCDGRADANILRFRGAAAGDSVHLDNHA
                     FLAFCYAYRHHISEDPLNDFLRVDGQPIYPQHGVPVQSPLMGVPYSGQYEGKLLWVHH
                     THDASLWPPQGVIYKRAVEDAQGPEKAREKFRLQWTQNAEHVPPMLLPTNPKRATTTW
                     LIDYMPVIEQGLVDLATWVEKGVPPAETTYTFSDGKVSLPPSAKARGGVQPVVEVTAN
                     GTVRADVKVGETVTFTAKAEAPDGAGTFTQAQWDFDASGAFALKAEVEPGQTELSLST
                     TTTFDAPGVYFVTCRVRLNRHGDPTARRQIENLASARVVVT"
BASE COUNT          518 a          988 c         1068 g          549 t
ORIGIN      
        1 ccggcaaacg aaaacgccgc cccgcgtgag cggagcggcg tttccatagc gtgtggacca
       61 gtcgtcaggt tcggcgctca gccttgcaag accagttggc ggcgtcatat acggcgttcg
      121 gcgttgcggc cttggagcgg tcgaacacgg cgacttcggg gaaacggcac agtatgaagt
      181 cgcgcgacga tccggtcggc ggcggcacca ggacgcccga caccatcttc gtcgccggat
      241 cggcgaacag cagctgctgg cgatcggcgc cgcggtgagc gacgacgggc ccgggagctt
      301 cgcccttctc cacccagttg atgagctcgg tcatcagcac atcgggaccg tcaccggggc
      361 cggtgcccga accgcagtga cccatgccgg ggatgcgata gaagctggcg aactggcgca
      421 gatttttcat gccgccgacc gaggtcgccg cgtccatgta gtactgctcc atttcggtat
      481 cgatgcagca gggatcgctg accccgttcc acatcagcac cttgccgccc ttggcatgat
      541 aggaagcgag gttggcgcta tagggccagc cgaagtcgag cttcttggtc gccgcccacc
      601 aggcgtcgat gacggcctgg tcgttgagat tcatcttcat gacgtcgaag tcggggccga
      661 acagcacctt ggccagcgaa ctgccgatca catagccggg cgaggacttg gccatgttct
      721 ccatcgtcgg ggcgtccgac cacggcggcg gagccatgcc catgaagccg gcccagacgc
      781 tcatgttcga gaccggaaag ccgaccttga tcaggcccgc ggggctgcgg gggccggcga
      841 cgatcgcctt gatgctcttg atctcgggtt gggtcaggca gtcggggccg tcacccttgg
      901 cgcaggcaaa ggcgtcgaaa tcgaccttgc acttagtgtg gtcctggatg atgtcgtcct
      961 tggcgccgtc gagctcatcg cagttggcgg tgatcttggc gtccagcatc acgaacttgg
     1021 ccggcgagac ccatgatccc ggctcccgcg ccatctgctg ggccgcgttg ataaaggcca
     1081 gcatcgtcgc tgaactgcgc ccgccgggcg cgccaagcag gacgccatca tagtcctcgg
     1141 gatgccgctc gatggcttcc gtcgtcatcc gaccgccgcc cgaacagccg gacgtataac
     1201 ggaagaagga ctcgacgtca tagtaggcgc gggtgatctg ctgggtcgcc accgccgtga
     1261 cgtgagcgcc ccggtcgatg tgatcgcgcg ccttggccgg atcggccagg aagctccagt
     1321 caaggatgtg gcccgtatgg ccggtgtccg tacccgcgac agcgaaaccc ttcagcagcg
     1381 gattgccggc cgggatctgc gagtcggacg gcacatagcc ggcggcgccg cccatgccga
     1441 tgaaatagta ccgcttctgc caaccggtgt cggggagttg gagccggaaa ctgaccttgt
     1501 tggggccagg gttgttggtg gtgatatagc cctcgatccg gcaatgcggc acgggcgccg
     1561 ccgtggtggt cgccgaggtg atcaccgtgt cggcgggggc gatggcctgg atcgcggccg
     1621 tctcgcaggt ccccgccgag gcggacgagc cgtagaacag cgccagcgcg gcgacggtgc
     1681 ccccggctgc cagcagtctg gatttcactg atagagtcac gattgtcctc cctggacgat
     1741 gtcgcccggg gccactcctc ttttggggag tgagcacccg ggaactggaa aaggtgccga
     1801 cggttcgccg gcggcgaaca ctcaggtgac gacgaccctg gccgaggcca ggttctcgat
     1861 ctgacgccgc gcggtcggat cgccatggcg gttcaggcgc acccggcagg tgacgaaata
     1921 aacgccgggc gcgtcaaagg tcgtggtggt cgacaggctc agttcggtct gacctggttc
     1981 aacctcggcc ttgagcgcga aggctccgct cgcgtcgaaa tcccactggg cctgggtgaa
     2041 cgttcccgcg ccgtcggggg cctcggcctt ggcggtgaag gtcacggtct cgccgacctt
     2101 gacgtccgcg cggacagtcc cgttggcggt gacctcgacg accggctgga cgccgccgcg
     2161 ggccttggcc gaagggggca ggctgacctt gccgtccgag aacgtatagg tggtttccgc
     2221 cggcggaacg cccttctcga cccaggtcgc cagatcgacc aggccttgtt cgatgaccgg
     2281 catatagtcg atcaaccagg tcgtggtggc gcgcttggga ttggtcggca gcagcatcgg
     2341 cggaacgtgt tcggcgttct gggtccattg cagccggaac ttctcgcgcg ctttctcagg
     2401 gccctgggcg tcctcgacgg cgcgcttgta gatgacgccc tgcggcggcc agagcgaggc
     2461 gtcgtgggtg tggtggaccc acaggagctt gccctcgtac tgaccggaat agggcacccc
     2521 catcaacggc gactgcaccg gcacgccatg ctgcggatag atgggttgac catcgacgcg
     2581 caggaaatcg ttgagcgggt cttcggaaat gtggtgacgg taagcgtagc agaaggccag
     2641 gaaggcgtgg ttgtcgaggt gaacgctgtc gccggccgcg gcgccccgga agcgcaggat
     2701 gttggcgtcg gcgcgcccgt cgcagaacca gatgtcgccg accgcgcggg tgcagaacag
     2761 ctgccgtccc gccgcgcccc cgctcttgat caggacgccg cagccggtgc tgtagccgcc
     2821 cttcagtccc ttgacctcga tcgcgacagg caggtcccag gcgccgctca tggacgccat
     2881 cagcaggatc atcgccttgg agtcggcgta ctcggggccg gcgaactcct cgcccatcag
     2941 gtcctgcgcc gtgatgatcc ggctgatcgg cagggtgagg tcgatcaggt cgtccttgac
     3001 cacgtctggc cggtcgtggc cgatgtatcc ggcctgcgtc cagaagttcg cgaaataatc
     3061 ggggtcttct tccagcagca tgctggcgat cgagcaccac agccaaatct gtcccatcgg
     3121 ctt
//
LOCUS       DAOMEO010000279         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3498_length_3112_cov_2.637880, whole genome shotgun sequence.
ACCESSION   DAOMEO010000279 DAOMEO010000000
VERSION     DAOMEO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_3112_cov_2.637880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..1417)
                     /gene="hisG"
                     /locus_tag="PLH31_07450"
     CDS             complement(455..1417)
                     /gene="hisG"
                     /locus_tag="PLH31_07450"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQR89088.1"
                     /translation="MSAPMIFAIPSKGRLKEQVEAWLAECGFKLEMTGGSRGYSAELS
                     GLPGVSVRLLSAGDIAAGLDAGELHLGVTGEDLLRERGDDMDSRVMLLRALGFCRADL
                     VVTAPKSWLDVDTMADIDEVGHAHLARTGRRLRVATKYVTQTRAFFARHGVADYRIVE
                     SSGATEGAPAAGAAELVVDITTTGATLAANGLKILTDGVILKSQAQLTASLIADWSPE
                     QLNALRRMLSVVEAKGRAARLATLVWPAEQDDTAQAAVASFVAKGGSRRANGALLATS
                     DLFDAAAALAGAGVEPVTVSRPDYVFESRSRVLDTLEHRLKGNI"
     gene            complement(1414..2541)
                     /locus_tag="PLH31_07455"
     CDS             complement(1414..2541)
                     /locus_tag="PLH31_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQR89089.1"
                     /translation="MRLERSIPADALDAIRAPLLAAGAVLTDAPVLQPLGLLLDLAGE
                     AMRSRLFVVSGDGGEEACLRPDFTVAIARAHLEAGAASGRYLYDGKAFRVAPRGSDRA
                     EEFLQIGVEAFEAGDPVAADAEIAALAWTSSAAGGRSDLSLVLGDVGLFGAFVDTLGL
                     APPLAVRLKRAFTRPRQLKIELDGGVEASAEQSRIAALLAGLPEGEASAVLQELWSLA
                     GIEPVGGRRPAEIAHRLVERAEAAKAGRLSVEQADKVRAFLAVSDRPGAALDAISALA
                     GPQRAALDAALDGWRSRLSGMAQRGAPEDRMVLTASFGRAFGYYDGYLFEVRSAALGD
                     ERPVAAGGRYDGLPARLGAERKTGAVGCMVRPARAYAGGGE"
     gene            complement(2546..>3112)
                     /locus_tag="PLH31_07460"
     CDS             complement(2546..>3112)
                     /locus_tag="PLH31_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HQR89090.1"
                     /translation="TGAVFEAELLLSTTDEKGAKVSFGSIGGGGRYDDLIARFTGQPV
                     PATGFSFGVSRLASALRAAGREPGGTARGPVVINFDQAHMGEYFSVAADLRNAGIPAE
                     VYLGTSGMRPQMKYADRRMAPAAIMLGGDEIAAGTVTIKDLDLGRELASGVADNAAWK
                     AERPGQQTIPRGELVSAVRKIISGSVEG"
BASE COUNT          518 a         1109 c         1028 g          457 t
ORIGIN      
        1 atctaaaagt ctcgcctgca gttgtgatgt gaaccttcac ggtgccggcc ttgcatcgaa
       61 cagcgcccgc gatcatctcc gcccatggca ggggtccgca agcacccgag cgccagcgca
      121 ggagggcaac ttgttgtttt tgactctttc gtcaaatatt gaagctggag cccagataca
      181 ccaaacccgc cgcgggggat aatcgcgacg ggctggctat ttctcgatca ttcgatctag
      241 gcgggcgttt cctccccgaa acgccgaagg aagggagctc tctgatgggc tctgttcttc
      301 cttgcaagcg ttagaaacgt cataattgag gcggtgtcaa cgcaccaaaa cccgtttctg
      361 taacggtttg agcgattttt tgacgaaacc gacgtagttt ggacgcggcg gtcaaactta
      421 tcaccgttct ctttgattat ttttgacgat agagtcaaat attacctttg aggcggtgct
      481 caagggtgtc cagtaccctg gagcgcgact cgaagacgta gtcgggacgc gaaacagtga
      541 ccggctccac ccccgccccg gccaaggccg cggcggcgtc gaacagatcc gaggtcgcca
      601 gcagggcgcc attggcccgg cgcgagccgc ccttggcgac aaagctggcg acagcggcct
      661 gggccgtatc gtcctgttcg gcgggccaga ccagggtcgc cagcctggcc gcccgcccct
      721 tggcctcgac caccgacagc atccgccgca gggcgttcaa ctgctcgggc gaccagtcgg
      781 cgatcagcga ggcggtcagc tgggcctggc tcttcaggat cacgccgtcg gtcaggatct
      841 tcaggccgtt ggccgccagg gtggcgccgg tggtggtgat gtcgaccacc agttccgccg
      901 ccccggccgc cggagcgccc tcggtggcgc cgctgctctc gacgatgcgg tagtcggcca
      961 cgccgtgacg agcgaagaag gcccgggtct gggtcacata tttggtcgcc acccgcagcc
     1021 ggcggccggt gcgggccaga tgggcgtgcc cgacctcgtc gatatcggcc atggtgtcga
     1081 cgtccagcca gctcttgggc gccgtgacca ccagatccgc ccggcaaaaa cccagggcgc
     1141 gcagcagcat cacccggctg tccatgtcgt cgccacgttc gcgcagcagg tcctcgccgg
     1201 tgacgcccag gtgcagctcg ccggcgtcga ggccggcggc gatgtcgccg gccgacagca
     1261 ggcgcaccga gacgccgggc aggcccgaaa gttcagcgct atagccgcgt gagccgccgg
     1321 tcatttccag cttgaagccg cattcggcca gccaggcctc gacctgctct ttcaggcggc
     1381 ccttggaggg gatggcgaag atcatgggcg cgctcattcg ccgcctcccg cataggctcg
     1441 cgccggacgg accatgcagc ccaccgcgcc tgtctttcgt tcagccccca accgggccgg
     1501 cagcccgtca tagcgaccgc cggccgccac gggccgctcg tcgccgaggg cggcgctgcg
     1561 cacttcaaag agatagccgt cgtaatagcc aaacgcccgg ccaaacgagg cggtcagcac
     1621 catgcgatcc tcgggcgcgc cgcgctgggc catgccggaa aggcgcgagc gccagccgtc
     1681 cagggccgcg tccagggccg cgcgctgcgg gccggcgaga gccgagatgg cgtccagcgc
     1741 cgcgcccgga cggtcgctga ccgccaggaa ggcgcggacc ttgtcggcct gttcgacgga
     1801 caaacgacca gccttggcgg cttcggcgcg ttcgaccagg cggtgggcga tctcggcggg
     1861 acggcgacca ccgaccggct cgatgccggc cagggaccac agctcctgca gcaccgccga
     1921 agcctcgccc tcgggcaggc cggccagcag cgcggcgatg cggctctgct cggcgctggc
     1981 ctcgacaccg ccgtccagct cgatcttcag ctggcgcggc cgggtgaagg cgcgcttcag
     2041 gcgcacggcc aaaggcggcg ccaggcccag cgtatccacg aacgccccga acagccccac
     2101 atcgcccagc accagagaaa ggtccgagcg gccgccggcg gctgacgagg tccaggccaa
     2161 agcggcgatc tcggcgtcgg cggcgacggg gtcgccggcc tcgaaggcct cgacgccgat
     2221 ctgcaggaac tcttcggccc ggtcgctgcc gcgcggcgcc acccggaagg ccttgccgtc
     2281 atagagatag cgccccgaag ccgcccccgc ctccaggtgc gcgcgggcga tggccacggt
     2341 gaaatcgggc cgcaggcacg cctcctcccc cccgtcgccg gacaccacga acaatcgcga
     2401 ccgcatggcc tcgccggcca ggtccagcag caggccaagc ggctgcagaa ccggcgcgtc
     2461 ggtcagcacg gcgccggccg ccagcagcgg ggcgcggata gcgtccagcg cgtcggccgg
     2521 aatggaacgc tcgagcctca tgcccttaac cctcaacgct accagagatg atcttgcgga
     2581 cagcggacac cagctcgccg cgcgggatcg tctgctggcc gggacgctcg gccttccagg
     2641 cggcgttgtc ggccacgccc gaggccagct cgcgacccag gtcaaggtcc ttgatcgtca
     2701 ccgtgccggc cgcgatctcg tcgccgccca gcatgatggc ggccggtgcc atccggcgat
     2761 cagcatattt catctgtggc cgcattcccg aggttccgag atagacctcg gccggaatgc
     2821 ctgcattgcg caggtcggcg gccaccgaga agtactcgcc catatgggcc tgatcgaaat
     2881 tgatcaccac cggaccgcgc gccgtcccgc caggctctcg ccccgccgcc cgcagggccg
     2941 aggccagacg cgagacgccg aacgagaagc cggtggccgg caccggctgg ccggtgaacc
     3001 gcgcgatcag gtcgtcatag cgcccgccgc cgccgatcga gccgaacgag accttggcgc
     3061 ccttctcgtc ggtggtggac agcagcagct cggcctcgaa caccgcgccc gt
//
LOCUS       DAOMEO010000280         3108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3503_length_3108_cov_2.323944, whole genome shotgun sequence.
ACCESSION   DAOMEO010000280 DAOMEO010000000
VERSION     DAOMEO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3503_length_3108_cov_2.323944"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PLH31_07465"
     CDS             complement(<1..1187)
                     /locus_tag="PLH31_07465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQR89091.1"
                     /translation="MTKSSQDELVFLPLGGSNEIGMNFNLYGFGPAHARKWIVVDLGV
                     TFGDQTTPGVEIILPDPEYIEAYADDILGIVLTHAHEDHLGAVHWLWPRLKAPVYATP
                     FTAFLLREKLRDARLLDEVSITEVPLGGKFSIGPFDLEMITLTHSIPEPNGLAIKTPL
                     GTILHTGDWKIDPEPQLGEATDIDAIRKLGDEGILAMVCDSTNVFVDGVAGSEADVRV
                     AMNKLISGLTGKIAVACFASNVARMDTVIRAAQAAGRKVCLAGRSMHRMSAAARSVGL
                     LVGIEPFITDDQAKYLPENEVLFLCTGSQGEARAALSRIAEGTHPHVKLSQGDHVVFS
                     SRVIPDNEIPIRNLQNKLADRGVRLHTERDTPGIHVSGHPCRDELKQMYQWARPEIAI
                     PTHG"
     gene            complement(1187..1966)
                     /locus_tag="PLH31_07470"
     CDS             complement(1187..1966)
                     /locus_tag="PLH31_07470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQR89092.1"
                     /translation="MMLLAIEQGNTNTMFAIHDGQSWIAQWRSATESTRTADEYVVWL
                     SQLLSMQGLGFRAIDACIISSVVPQSIFNLRNLSRRYLNVEPLVIGENAKLGVDVRIE
                     KPSEAGADRLVNAVGANMVYPGPLIVIDSGTATTFDIVAADGAFEGGIIAPGINLSMQ
                     ALHEAAAKLPRIAIQRPARVVGKDTVGAMQSGVFWGYIALIEGLITRIKAERAEPMTV
                     VATGGVASLFEGATDSIDHFDPDLTIRGLLEIHRRNTILET"
     gene            complement(1963..2724)
                     /locus_tag="PLH31_07475"
     CDS             complement(1963..2724)
                     /locus_tag="PLH31_07475"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQR89093.1"
                     /translation="MTGVPSLSKGPPVVVLDEIDSTNAEARRRAEAGEAGPVWLLGLR
                     QTAGRGRRGRAWETGAGNLAATLLFATDKPPAEAAQVSFVAALAVADLLAVYAPASLI
                     SLKWPNDPLLGGLKVSGILVESGTRPTGGLWIAVGIGVNLARKPIDSERPTTALTTHR
                     AAPPPSPVEAIEVLSDAFERWSRVWLTLGFPAIADAWTARAHGLGEPCVARLGTETVE
                     GVAEGLDGDGALRLRLSDSRLRRITVGDVFFGEAT"
     gene            complement(2726..>3108)
                     /locus_tag="PLH31_07480"
     CDS             complement(2726..>3108)
                     /locus_tag="PLH31_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQR89094.1"
                     /translation="MAGLIKERPGIALAMTAFSLSALGLPPFSGFWSKFYVFKAALGT
                     GDVMMQWAAVLGLVGSVVGAFYYLRLIKSIWFDASKGAVDAPAPAARAVGFAAALFAF
                     PVVLVALIWLDPAAKAAAAAFGHA"
BASE COUNT          559 a          998 c         1050 g          501 t
ORIGIN      
        1 ccatgggtcg gtatggcgat ctcgggacgc gcccactggt acatctgttt cagctcgtcg
       61 cggcacgggt ggccactgac gtggatgccg ggcgtgtcgc gttcggtgtg cagccgcacg
      121 ccacggtcgg ccagcttgtt ctgcaggttg cggatcggga tctcgttgtc cggaatgacc
      181 cgcgagctga agaccacgtg atcgccctgt gacagcttca cgtgcggatg ggtgccctcg
      241 gcgatacgcg acagggccgc cctcgcctcg ccctggctgc cggtgcagag gaataggacc
      301 tcgttctcgg gcaggtactt ggcctggtcg tcggtgatga agggctcgat acccaccagc
      361 aggccgaccg agcgcgcggc cgcgctcatg cgatgcatcg agcgaccggc caggcacacc
      421 ttccgaccgg cagcctgggc ggcgcggatc acggtgtcca tgcgcgcgac gttcgaggcg
      481 aagcaggcta cggcgatctt gcccgtcagg cccgagatca gcttgttcat ggccacccgg
      541 acatcggcct cggaaccggc cacgccatcg acaaacacat tggtgctgtc gcagaccatg
      601 gccaggatgc cttcgtcgcc caacttgcgg atggcgtcga tgtcggtggc ttcgcccaac
      661 tggggctccg gatcgatctt ccagtcaccg gtgtgcagga tcgtgccgag cggggtcttg
      721 atcgccaggc cgttcggctc ggggatcgag tgggtcaacg tgatcatttc cagatcgaac
      781 ggaccgatcg agaacttgcc gcccagcggc acctcggtga tcgacacctc gtccagcagc
      841 cgcgcgtcgc gcagcttctc gcgcagcagg aaggccgtga acggggtggc gtagaccggc
      901 gccttcaggc ggggccaaag ccagtgcacc gcgcccagat ggtcttcgtg ggcatgggtc
      961 aggacgatgc cgagaatatc gtcggcatag gcctcgatat attcggggtc gggcaggatg
     1021 atctcgacgc cgggcgtcgt ctggtcgcca aaggtgacgc ccagatcgac cacgatccac
     1081 ttgcgagcgt gggccgggcc aaagccatac agattgaagt tcatgccgat ctcgttcgac
     1141 ccgcctagcg gcaggaaaac gagttcgtct tgggaggatt ttgtcatcag gtctctaaga
     1201 tggtgttgcg gcggtgaatt tcgaggagac cacgaatggt cagatcggga tcgaagtggt
     1261 cgatgctgtc ggtcgcgccc tcgaacaggg atgcgacgcc gccagtggcg accacggtca
     1321 tgggttcggc gcgttcggcc ttgatacggg taatcaggcc ctcgatcagg gcgatatagc
     1381 cccagaagac acctgactgc atggctccca ccgtatcctt gccaaccaca cgggccggtc
     1441 tttgaatggc gatacgcggt agcttagcgg ccgcttcgtg cagggcctgc atggaaagat
     1501 tgatgccggg tgcgataatg ccgccttcga aggcgccatc ggcggccacg atgtcgaagg
     1561 tggtggccgt gccgctgtcg atcacgatca gcggacccgg atagaccatg ttggcgccca
     1621 cagcgttgac aaggcggtca gcgcccgcct cggagggctt ttcgatacga acatcgacgc
     1681 ccagtttggc gttctcgccg atcaccaggg gctcgacgtt cagatagcgg cgcgacaggt
     1741 tgcgaaggtt gaaaatcgac tgcggcacaa cgctggagat gatgcaggcg tcgatggccc
     1801 ggaaacccag cccctgcatc gacagcagtt gcgacagcca cacgacatac tcgtcggccg
     1861 tccgcgtgct ctcggtggcc gagcgccatt gggcgatcca gctctggcca tcatggatgg
     1921 cgaacatggt gttggtattg ccctgctcga tggccagcag catcaggtcg cctcgccgaa
     1981 gaagacatcg ccgaccgtga tgcgccgcaa gcggctgtca gacaaccgga gccttagggc
     2041 gccgtcgcca tcaaggccct cggccacgcc ctcgacggtc tcggttccaa gcctggccac
     2101 gcacggttcg cccaggccgt gggcgcgcgc cgtccaggcg tcggcgattg cgggaaagcc
     2161 caaggtcagc cagacgcgcg accagcgctc gaacgcgtca gacagcacct cgatcgcctc
     2221 gaccggcgat ggcggcgggg cggcgcgatg ggtggtcagc gccgtggtgg gacgctcgct
     2281 gtcgatgggc ttgcgggcca ggttcacgcc gatgccgacc gcaatccaga gcccgccggt
     2341 cgggcgagtt ccagactcga caaggatgcc gctgaccttc aatccgccca gcaagggatc
     2401 attgggccat ttcaggctga tcagcgacgc cggcgcatag accgccagca ggtcagccac
     2461 ggccaaagcc gccacgaacg acacctgggc ggcctcagcg ggcggcttgt cggtggcaaa
     2521 cagcagcgtg gcggcaaggt tgcccgcgcc ggtttcccac gcccgcccac gtcgcccccg
     2581 tccggcggtc tggcgcaagc ctagcagcca gaccggcccg gcctcgcccg cctccgcgcg
     2641 gcgccgggcc tcggcattgg tggagtcgat ctcgtcgaga acgacgacgg gcggcccttt
     2701 ggaaagtgag ggaacgcccg tcacgtcacg cgtggccaaa ggccgcggct gcggcctttg
     2761 cggccgggtc gagccagatc agcgcgacca gcaccaccgg gaaggcgaaa agggcggcgg
     2821 cgaaaccgac ggcacgcgcc gccggggccg gagcatcgac ggcgcccttt gaagcgtcga
     2881 accagatgct cttgatcagg cgcagatagt agaaggcgcc aacgacgctg ccgaccagac
     2941 cgagaacggc ggcccactgc atcatcacgt cgcccgtgcc gagcgcggcc ttgaagacat
     3001 agaacttcga ccagaagccc gagaacggcg gcaggcccag ggccgacagc gagaacgcgg
     3061 tcatggccag ggcgataccc ggccgttcct tgatcaggcc ggccatgt
//
LOCUS       DAOMEO010000281         3101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3509_length_3101_cov_3.123441, whole genome shotgun sequence.
ACCESSION   DAOMEO010000281 DAOMEO010000000
VERSION     DAOMEO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_3101_cov_3.123441"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..161
                     /locus_tag="PLH31_07485"
                     /pseudo
     CDS             54..161
                     /locus_tag="PLH31_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            161..769
                     /locus_tag="PLH31_07490"
     CDS             161..769
                     /locus_tag="PLH31_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR89095.1"
                     /translation="MPRIPGQIDIAKTEAILDAAVEVIGARGLGAPMEAIARRAGVSK
                     QTVYNHYGSKAELVRALMARRVDRITAPLRDPGAVDNPRLALQAYARSMLEAVLVNKG
                     YSVMRVIIQGAGEMPDIAHEVFEAGPRQARRQLAAYLETETRVGRLHVENFDQAAEFF
                     SGMVLGQSQLRALLSLPLDKSEAELDSLAGEAAKRFMRAYAP"
     gene            complement(935..1750)
                     /locus_tag="PLH31_07495"
     CDS             complement(935..1750)
                     /locus_tag="PLH31_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HQR89096.1"
                     /translation="MADDYAFPKGELMRGKKGLVMGVANHNSIAWGIASQLAAQGAEM
                     AFTYQGEDLERRVRPLAESIGVSTMIPADVTDDASMDAAFAAIEEKFGTLDFVVHSVA
                     FANKNELKGSFVENTTREGFLTAMNISAFSFVDVAKRASRIMPNGGSLITMTYLGSER
                     TIPNYNTMGVAKAALEACTRYIARDLGPKGIRVNAISAGAMRTLSLAGISGGRGMIAQ
                     GRAFSAMKEDTSMEGVAGCALWLASDLGRSTTGEVVHVDAGFHMMGMPDEAEA"
     gene            complement(1871..3088)
                     /gene="fabB"
                     /locus_tag="PLH31_07500"
     CDS             complement(1871..3088)
                     /gene="fabB"
                     /locus_tag="PLH31_07500"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HQR89097.1"
                     /translation="MRRVVVTGLGIVSSIGNTASEVLASLREARSGVVAAPDYAELGF
                     RCQVHATVKLDAWEALVDRRAARFLAPGTAYAHIAMEQAIADSGLEESEISNERTGLI
                     VGSGGPSTRVIVEAAATTKEKGPKRIGPFAVPKAMSSGPSAVLSTWFKIRGINYSISS
                     ACATSAHCIGAGAEQIQMGKQDIVFAGGCEELDWTLSNLFDAMGAMSSNFNDRPTVAS
                     RAYDRDRDGFVIAGGAGIVVLEEYEHAKARGAKIYGEVVGYAANSDGFDMVAPSGEGA
                     ARCMKLAMAEAGNRTIDYLNPHGTSTPVGDSKEMGAVREVFGDKAPLISSTKSLTGHS
                     LGAAGAQEAIYSILMLNNDFAAQSANIENLDPEFADLPILLQRSDKPLTTVMSNSFGF
                     GGTNGTLIISKAD"
BASE COUNT          513 a         1018 c         1022 g          548 t
ORIGIN      
        1 cgctatcgca gaaatggcaa ggaagctaaa tttagatatt gcagtcgatc ttgggcgaca
       61 gtttcaccct cctggcgatc ggctgcttcg gcgcggcctt tgtttcgctt ctggccaggc
      121 ccgccgccgt ctcggccccg cccccttcgg acgcgcattg atgcccagga tccccggaca
      181 gatcgacatc gccaagaccg aagcgatcct cgacgccgcc gtcgaggtaa tcggcgcgcg
      241 cggcctgggg gcgccgatgg aggcgatcgc ccgacgcgcc ggggtctcaa agcagacggt
      301 ctacaaccac tatggctcca aggccgagct ggtccgcgcc ctgatggcgc gccgggtgga
      361 ccggatcacc gcgcccttgc gcgaccccgg cgccgtcgac aacccccgcc tcgcgctgca
      421 ggcctatgcc cgttcgatgc tggaggccgt tctggtcaac aagggctatt cggtcatgcg
      481 ggtgatcatc cagggcgcag gagaaatgcc cgacatcgcc catgaggtgt tcgaggccgg
      541 tccccgccag gcccggcgcc aactggccgc ctatctggag accgaaaccc gcgtcggccg
      601 actgcacgtc gaaaacttcg accaggccgc cgagttcttt tcgggcatgg ttctgggcca
      661 aagccagctg cgagccctgc tgagcctgcc gctcgacaag tccgaggccg aactggacag
      721 cctggcgggc gaggccgcca agcgattcat gcgcgcctac gcaccctaga gccctttccg
      781 acctgatggc ttcatcaggg tcgggcgaaa agggctctaa atcaaaagtt tagagcattt
      841 ttcgatccga taaccgtttc acacttatcg gaaaatgctc tagaaaaagg cccccgccga
      901 caaggcgggg ccctatcctg ccggaagcgg ccgatcaggc ttccgcttcg tccggcatgc
      961 ccatcatgtg gaagccggcg tcaacgtgga cgacctcgcc cgtggtcgaa cgacccaggt
     1021 ctgaagccag ccacagggcg cagcctgcga cgccttccat cgaggtgtct tccttcatgg
     1081 cgctgaacgc gcggccctgg gcgatcatgc cccggccgcc cgagatgccg gccagcgaca
     1141 gggtgcgcat ggcgcccgcc gagatggcgt tgacccggat acccttggga cccaggtcgc
     1201 gggcgatgta gcgggtgcag gcctccagag ccgccttggc cacgcccatg gtgttgtagt
     1261 tggggatggt ccgctccgac cccagatagg tcatggtgat cagcgaaccg ccgttgggca
     1321 tgatccgcga cgcgcgcttg gcgacgtcga cgaagctgaa ggccgagatg ttcatggccg
     1381 tcaggaagcc ttcccgcgtg gtgttctcga cgaacgagcc cttcagctcg ttcttgttgg
     1441 cgaaggccac cgaatggacc acgaaatcca gcgtgccgaa cttttcctcg atggcggcga
     1501 aggccgcgtc catcgaggcg tcatcggtga cgtcggccgg gatcatggtc gagacgccga
     1561 tactctcggc cagcggccgc acgcggcgct ccaggtcttc gccctgatag gtgaacgcca
     1621 tctcggcgcc ctgggcggcc agctgcgagg cgatgcccca ggcgatggaa ttgtgattgg
     1681 ccacgcccat gacgaggccc ttcttgcccc gcatcagctc gcccttgggg aatgcgtaat
     1741 cgtcggccat gggttggcgc tccgtgctct gggataggac ggccagcccg catgggccgc
     1801 cgtcacccgt catcgtgggg atttagctat cagggtcagc gcgccgctgg aaccctccag
     1861 cggcgtggga tcaatcggcc ttgctgatga tcagcgtgcc gttggtgccg ccaaagccga
     1921 agctgttgga catcaccgtg gtcaggggct tgtccgagcg ttgcagcagg atcggcaggt
     1981 cggcgaactc gggatccagg ttttcgatat tggcgctctg ggcggcaaaa tcgttgttga
     2041 gcatcaggat cgagtagatc gcctcctgcg cgccggccgc gcccaggctg tgacccgtca
     2101 gcgacttggt cgacgagatc agcggggcct tgtcgccgaa cacctcacga accgcgccca
     2161 tttccttgct gtcgcccacc ggcgtggagg tgccgtgcgg gttcagatag tcgatggtgc
     2221 gattgccagc ttcggccatg gccagcttca tgcagcgggc cgcgccttcg ccggaagggg
     2281 cgaccatgtc gaagccgtcg gaattggcgg cgtagccgac cacttcgcca tagatcttgg
     2341 cgccgcgcgc cttggcgtgc tcgtactctt ccaggacgac gatgcctgcc ccgccggcga
     2401 tcacgaagcc gtcgcggtcc ctgtcgtagg cgcggctggc cacggtgggg cggtcgttga
     2461 agttgctgct catcgcgccc atggcgtcga acaggttgga cagggtccag tccagctcct
     2521 cgcagccgcc ggcgaagacg atgtcctgct tgcccatctg gatctgctcg gcgcccgcgc
     2581 cgatgcagtg ggcgctggtg gcgcaggccg agctgatcga atagttgatc ccgcggatct
     2641 tgaaccaggt cgacagcacc gccgaggggc ccgaactcat ggccttgggc accgcgaacg
     2701 ggccgatgcg cttgggaccc ttttccttgg tcgtggcggc ggcctcgacg atcacccggg
     2761 tggacggacc gccggagccg acgatcaggc cggtgcgctc gttggagatc tcagactctt
     2821 cgagaccgga atccgcgatc gcctgttcca tggcgatgtg ggcgtaggcc gtgccggggg
     2881 ccaggaagcg cgccgcgcgg cggtcaacca gggcttccca cgcgtcgagc ttgaccgtgg
     2941 cgtgcacctg gcagcggaag ccaagctccg catagtcggg cgcggcgacc acgccggacc
     3001 tggcttcccg cagcgaggcg agcacctcgc tggcggtgtt gccgatggac gagacgatac
     3061 ccagtcctgt tacgacgacg cgacgcatga aacttcctct t
//
LOCUS       DAOMEO010000282         3101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3511_length_3101_cov_2.506238, whole genome shotgun sequence.
ACCESSION   DAOMEO010000282 DAOMEO010000000
VERSION     DAOMEO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_3101_cov_2.506238"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLH31_07505"
     CDS             complement(<1..405)
                     /locus_tag="PLH31_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQR89098.1"
                     /translation="MLIQQGRAVGVEVVRKGATHWFGVAHEVILCLGAIQTPTVLMRS
                     GIGDEAELRAHDIKVVQSLPGVGANLQDHVSFGCIWEYREPIAPRNSGSEATLYWKSE
                     AALTEPDLLFCQVEFPVPSPEAAPRGAPEHGWT"
     assembly_gap    412..421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<422..664)
                     /locus_tag="PLH31_07510"
     CDS             complement(<422..664)
                     /locus_tag="PLH31_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQR89099.1"
                     /translation="MVWARGHRSDWDHFAKESGDASWGYDAVLGLYREIENWQGSPDP
                     LRRGKGGPVYVQSAPKPGPAAYAMVDAARELGIPTYA"
     gene            644..1552
                     /locus_tag="PLH31_07515"
     CDS             644..1552
                     /locus_tag="PLH31_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQR89100.1"
                     /translation="MAARPDHDVDTGAPSCFVDAYPEPFSALLNIGGRSPAAVVIDAA
                     SLPMASDLLRALASDSALSQTAILLVGPKVETSAFAGPAKGRVSFVEDLDALAEAAET
                     AVRGPAAIHVGPEAGSLFPLPGNEAQRLLALERSGLVETPPEDAFDRLTWLAAQTLGM
                     PISLITLLTPTRQWFKSRHGLDMADTPRSWAFCNHTILQQRCMVSEDLSADPRFSDNP
                     AVVDAPHFRFYAGCPIVDPDGFTLGSLCVIDTQPRSLNDTEQAILTNLASLVSDEIKL
                     RVTDGRLKRAMDALDRKPRWGGRTST"
     gene            complement(1549..>2269)
                     /locus_tag="PLH31_07520"
     CDS             complement(1549..>2269)
                     /locus_tag="PLH31_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HQR89101.1"
                     /translation="AFTPNLVLGLALSADHADVDLASGGKLKVKSYSPAAYAAWNSGA
                     FHLDGYAAYTTVDYDARRNLRIGGATLSATADPDGKAYTGAIGAGYRLGSGAFTFGPI
                     ADLVVTRVKVDGYVETNAQDFGSTLQSRDRDSVRLGLGVSGRYAMEKAWGVMVLTGKA
                     RAVQELADKRDIFRAAFSAQPDAPFDVRGPKTGVTYGAFDLGLTAVTKAGLRIGVTYA
                     PRLDGDGLVDQSALLSIAKSF"
     assembly_gap    2270..2279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2280..3061)
                     /locus_tag="PLH31_07525"
     CDS             complement(<2280..3061)
                     /locus_tag="PLH31_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89102.1"
                     /translation="MPISNMSRARLLTMALLGSVIASSAARAQAVIDKAGDFIPSYTG
                     PKTADLDVIAAKASYNGTIFRLDATTAGPIGQTAGGVYVWGFNRGAGTARFGAIAPNV
                     TFDSVIIIDPKGTVTVRDLVTGAATVLPTDSVTVSGNTISVSVPANLLSSQGLKTAQF
                     LANLWPRSGLVGNETISDFAPDNTDTRLVLDFPLPVEASAQTELVIDGASEHFNQVQG
                     RLSARRDGDAQRYGGFISAGARFGDHGLGDSTIGDQDTSILSA"
BASE COUNT          500 a         1033 c         1042 g          506 t
ORIGIN      
        1 ggtccagccg tgctcgggcg cgccgcgagg ggcggcttcg gggctcggaa ccggaaactc
       61 gacctgacag aacagcaaat ccggctcggt gagggccgcc tcggatttcc agtagagggt
      121 ggcttcgctg ccgctgtttc gcggcgcgat cggctcgcga tactcccaga tgcagccgaa
      181 cgagacgtga tcctgcaggt ttgcgccgac gcccgggagg ctttgcacaa ctttgatgtc
      241 gtgggcgcgc agttccgcct cgtcgccgat gcccgaccgc atcagaacgg tcggcgtctg
      301 gatcgcgccg aggcagagaa tgacctcgtg ggcgacaccg aaccagtgcg tcgcgccttt
      361 gcgaaccacc tcgacgccga cggctcgacc ctgctggatc agcacccggt tnnnnnnnnn
      421 nggcataggt cggaatgcca agctcccggg cggcgtcgac catggcgtag gccgccggac
      481 cgggtttggg ggccgattgc acatagaccg gaccgccctt gccgcgccgc agagggtcag
      541 gcgagccctg ccagttctcg atctcccggt agaggcccag cacggcgtcg tagccccagg
      601 aggcgtcgcc gctttccttg gcaaaatgat cccagtcgct gcggtggccg cgcgcccaga
      661 ccatgacgtt gatactggag ccccctcctg cttcgtcgac gcctatccgg agccgttctc
      721 cgcccttctg aacatcggcg gccgctcgcc ggccgccgtg gtcatcgacg cggcctccct
      781 ccccatggcc tcagacctgc tgcgggccct ggcttcggat tcggcgctgt cgcagacggc
      841 gatcctgctc gtcggcccca aggttgaaac ctcggctttc gccggcccgg ccaaaggcag
      901 ggtcagcttc gtcgaggatc tggacgccct ggccgaggcc gccgaaacgg cggtgcgagg
      961 accggcggcg atccatgtgg gacctgaagc agggtcgctg ttcccgctgc ccggcaacga
     1021 agcccaacgc ctcctggcgc tcgagcgctc gggcctggtc gaaacgcccc ccgaggacgc
     1081 cttcgacagg ctgacctggc tggccgcgca gacgctgggc atgccgatct cgctgatcac
     1141 cctgctgacc ccgacccgac agtggttcaa gtcgcgccat ggcctggaca tggcggacac
     1201 gccccgcagc tgggccttct gcaaccacac catcctgcag cagcgctgca tggtgtcgga
     1261 ggatctgtcc gccgatccgc gcttcagcga caatccggct gtcgtcgacg cgccgcactt
     1321 tcggttctac gccggttgcc cgatcgtcga tcccgacggc ttcacgctgg gctcgctctg
     1381 cgtgatcgac acccagccgc gcagcctgaa cgacaccgag caggccatcc tgaccaacct
     1441 cgcgtccctg gtgtcggacg agatcaagct gcgcgtcacg gatgggcggt tgaaacgggc
     1501 catggacgct ctggaccgca agccgcgctg gggcggccgg acctctacct agaaactctt
     1561 ggcgatcgat agcagcgctg actggtcgac aaggccgtcg ccatcgagtc gcggcgcata
     1621 ggtgacgccg atgcgcagcc ccgccttggt gaccgccgtc aggccaagat cgaacgcccc
     1681 ataggtgacg ccggttttcg gaccgcgcac atcgaaggga gcgtcgggtt gggcgctgaa
     1741 ggcggcccgg aagatatcgc gcttgtcggc gagttcttgc acggcgcggg ccttgccggt
     1801 cagcaccatc acgccccacg ccttctccat cgcatagcgt ccgctcacac ccagccccag
     1861 gcggacgctg tcgcgatcgc gagactgaag ggtcgagccg aagtcctggg cgttggtctc
     1921 gacatagccg tcgaccttta ccctggtcac caccagatcg gcgatgggcc cgaaggtgaa
     1981 ggccccggaa ccaaggcgat agccggcgcc gatcgcgccg gtataggcct tgccatcggg
     2041 gtcagccgtg gcggaaagcg tcgcgccgcc gatccgcagg ttacgccggg cgtcgtagtc
     2101 gacggtcgtg taggcggcat agccatccag atggaaggcg ccgctgttcc aggcggcata
     2161 cgccgccggc gagtagctct tgaccttgag tttgccgccg gacgccaggt cgacatcagc
     2221 gtggtcggcc gacagcgcca gacccaggac cagattgggc gtgaaggcgn nnnnnnnnng
     2281 ccgacaggat cgaggtgtcc tggtcgccga tggtcgagtc tcccaagccg tgatcgccga
     2341 accgggcgcc ggcgctgatg aagccgccat agcgttgcgc gtcgccatca cgccgcgccg
     2401 agagccgccc ctgcacctgg ttgaagtgct ccgacgcgcc gtcgatgacc agttcagttt
     2461 gggcggaggc ctccaccggc agtgggaagt ccagaacgag gcgagtgtcg gtgttgtccg
     2521 gcgcgaagtc cgagatcgtc tcgttgccca ccagcccgct gcgcggccag agattggcca
     2581 ggaactgcgc ggtcttgaga ccctgggagc tcaagagatt ggccggcacg gacacgctga
     2641 tcgtgttgcc cgacacggtg accgaatccg tcggcagcac ggtggccgcg ccggtgacga
     2701 ggtcgcgcac cgtcaccgtt cccttcgggt cgatgatgat caccgagtcg aaggtgacgt
     2761 tcggggcgat ggcgccaaag cgcgcggtcc ccgcgccgcg attgaagccc cagacataga
     2821 cgccacccgc ggtctggccg atcggcccgg cagtcgtggc gtcgagccgg aagatcgtac
     2881 cgttatagct ggccttggcg gcgattacat cgaggtcggc ggtcttgggc ccggtatagc
     2941 tgggaataaa gtcgccggcc ttgtcgatga cggcctgggc tcgggcggcg ctggaggcga
     3001 tcactgaacc cagcagcgcc atcgtcagga ggcgggcgcg ggacatgttc gagatgggca
     3061 tggggagaga ctctcagctt ttcgatgttg atgggcgtag c
//
LOCUS       DAOMEO010000283         3100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3512_length_3100_cov_2.758949, whole genome shotgun sequence.
ACCESSION   DAOMEO010000283 DAOMEO010000000
VERSION     DAOMEO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3512_length_3100_cov_2.758949"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..532
                     /locus_tag="PLH31_07530"
     CDS             17..532
                     /locus_tag="PLH31_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR89103.1"
                     /translation="MDAPLVRAARAGSQAAFARLVAIHETPLRGFLRKSGWIDADDIA
                     QDAFVAAWTSLGRLRADEGFRAWLYGIAWRKALDNRRAASRSARRDEDWRRRDALEGA
                     RVVAPEDRIALQAALDTLPEDQRACVTLCLGQGWSHPEASEALGLPLGTVKSHVTRGR
                     ERLLAVLGEPS"
     gene            529..819
                     /locus_tag="PLH31_07535"
     CDS             529..819
                     /locus_tag="PLH31_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89104.1"
                     /translation="MTPDDRLSAFLGEAPAPRACDVFVAEVMEAVERRMFVDRLMAGG
                     AAAFAGGAVLWACSPALNLAVDILAPTLVPVAGMLVLAAAAAILADRLLPRK"
     gene            927..1310
                     /locus_tag="PLH31_07540"
     CDS             927..1310
                     /locus_tag="PLH31_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HQR89105.1"
                     /translation="MDGIWVPIIMFLVIGAIVIVPIWLKSRERIEMQSTLRAAIDKGQ
                     PVPPEVIEALTRNVKVPPTSLSDIRTGVIWLAVGIGIGLFSYFADFADHDVDFPGLGI
                     ACIPAVIGLAFIVLSFFNPNKPKSA"
     gene            1338..1910
                     /locus_tag="PLH31_07545"
     CDS             1338..1910
                     /locus_tag="PLH31_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR89106.1"
                     /translation="MSRDRPPTGHDVELAALAAAGDRRAYGELVRRHGSAVRGLLRRM
                     GADSATADDMAQDAFLAGFEQVADYRGEGAFGGWIKRIAARCYLRKVKREARMILTDE
                     AQDGDDPTALNEPRTANRIDLDDALKALSKGERLCVSLCYGGDWSHAEVAEALNIPVG
                     TVKSHVKRGLDKLRAKLAPPTESARREAHG"
     gene            1903..2283
                     /locus_tag="PLH31_07550"
     CDS             1903..2283
                     /locus_tag="PLH31_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89107.1"
                     /translation="MAEPDFEAQLTRLYNEPPAFADAALFAAQVAANLDRGWALRRIL
                     IGGAGIAAGVIAAAQLITTRFSADLQALSRDGTRDLNADIGVALDKLNQVVSTPASVE
                     ALWLAAGLALLALGFAVTRAMEEL"
     gene            2382..>3100
                     /gene="panB"
                     /locus_tag="PLH31_07555"
     CDS             2382..>3100
                     /gene="panB"
                     /locus_tag="PLH31_07555"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQR89108.1"
                     /translation="MSSHRDEIVRRLAAPDIAARKGGVPIVCLTAYTAPVAAALDDAC
                     DLLLVGDSLGMVVHGLPNTVGVTMDMMILHGQAVMRGSRKAMVVVDMPFGSYEASPEV
                     AYANAVRIMKETGAQAVKVESGPTVPATIEYLTKRGVPVMGHVGLRPQAVLVDGGFKA
                     KGKDEAGRALILEEARRTADAGAFAVVVEGVAESLAREITAAIAPPTIGIGASAGCDG
                     QILVTDDMLGLFDWTPKFVRR"
BASE COUNT          479 a          994 c         1066 g          561 t
ORIGIN      
        1 gaggcccgaa agtctgatgg acgcgccgct cgtccgagcg gcgcgggccg gatcccaggc
       61 ggcgttcgcc cgtctggtcg cgatccacga gacgcccttg cgggggttcc tgaggaaaag
      121 cggctggatc gacgccgatg acatcgctca ggacgccttc gtggcggcct ggacgagcct
      181 gggacggctg cgcgccgacg agggttttcg cgcctggctc tatggcatcg cctggcgcaa
      241 ggcgctggac aaccgccgcg ccgccagccg ctcggcccgg cgcgacgagg actggcggcg
      301 ccgggatgct ctggaggggg ccagggtggt cgccccggaa gatcgcatcg ccctgcaggc
      361 cgcgctcgat accttgccgg aagaccagcg cgcctgcgtt acgctttgcc tgggtcaggg
      421 ctggtctcat cccgaagcct ccgaagctct gggcctgcca ctgggcactg ttaagtcgca
      481 tgtaacccgc ggtcgtgaga ggctgctggc cgtactggga gagccgtcat gacccccgat
      541 gatcgtttgt cggcttttct gggcgaagct cccgcgccgc gagcgtgcga cgtgttcgtg
      601 gccgaggtga tggaggcggt ggagcgccgg atgttcgttg atcgtctgat ggcgggcggg
      661 gcggcggctt tcgcgggcgg cgccgtgctg tgggcctgtt cgccggccct gaacctggcg
      721 gtcgatatcc tggcgcccac ccttgttccg gtggcgggca tgctggtcct ggccgccgct
      781 gcggccatcc tggccgaccg gttgctgccg cggaaatagc ttacgaaagt ttcatcccgc
      841 cgggcgcctc caaacgcctg tcccggcgcc tcttatagtc agaaggcagc gataacgatg
      901 tcgcgcccac cgtgaggaat ttcgccatgg acggcatctg ggtccctatc atcatgtttc
      961 tggtcatcgg cgcgatcgtg atcgtgccga tctggctgaa gagccgcgag cgcatcgaga
     1021 tgcaatcgac gctgcgcgcg gcgatcgaca agggacagcc ggttccaccg gaagtgatcg
     1081 aagccctgac ccgcaacgtg aaggtcccgc cgacctcgct gagcgatatc cgcaccggcg
     1141 tgatctggtt ggctgtcggt atcggtatcg gcctcttcag ctacttcgcc gattttgccg
     1201 atcatgacgt tgacttcccg ggtctcggca tcgcctgcat tcccgcggtg atcggtctgg
     1261 ccttcatcgt tctgagcttc ttcaacccga acaagcccaa gtcggcttga tcacgggtct
     1321 cggggagggg gaagagcatg tcgagggacc gcccaccaac gggccacgat gtcgagctcg
     1381 ccgctctcgc ggcggcgggc gaccggcgcg cctatggcga gctcgtcagg cgacatggct
     1441 cggcggttcg ggggctgctg cgccgcatgg gagccgactc ggcgacggcc gatgacatgg
     1501 cccaggacgc cttcctggct ggcttcgagc aggtggcgga ctatcgcggc gaaggcgctt
     1561 tcggcggctg gatcaagcgg atcgcggcgc gttgttatct gcgcaaggtc aagcgcgaag
     1621 cccgcatgat cctcaccgac gaggcccagg acggcgacga cccgacggcg ctgaacgaac
     1681 cccgcacggc caaccgtatc gacctggacg acgctttgaa ggctttgtca aaaggcgaac
     1741 ggctctgcgt ctcgctctgc tacggagggg actggtcgca cgccgaggtc gctgaggcct
     1801 tgaacatccc ggtcggcacg gtgaaatccc atgtcaaacg tggtttggat aaactcaggg
     1861 ccaaactggc cccacccact gaaagcgcga ggagggaagc tcatggctga acccgatttc
     1921 gaagcgcagc tgacacggct ctacaacgag ccgccggcct ttgcggacgc cgcgctgttc
     1981 gccgcccagg ttgcggccaa tcttgatcgc ggctgggcgc tgcggcggat actgattggc
     2041 ggcgcgggta tcgctgcggg cgtcatcgcg gcggcgcaac tgatcacgac ccgtttttcg
     2101 gccgacctgc aggctttgtc gcgagacggc acgcgggacc tgaacgccga catcggcgta
     2161 gcgctggaca aactcaatca ggtcgtttcc accccggcca gtgtcgaagc gctgtggctt
     2221 gctgcgggac tggctcttct ggcgctgggc tttgcggtga cccgagcgat ggaagagctc
     2281 tgagggctcg ccactgatcg tcgttcatgt tgcagcgcgg cgcgatctga ccgacaacag
     2341 cgcgtcctcg aagcgccgag gacctgttct ggagcatggt tctgtcttcg caccgtgatg
     2401 agatcgtccg ccgccttgca gcccccgaca tcgccgcgcg caagggcggc gtgccgattg
     2461 tctgcctgac ggcctatacc gcgcccgttg cggcggcgct ggacgacgcc tgtgacctcc
     2521 tgctggtcgg tgactccctg ggcatggtcg ttcacggcct gcccaacacc gtcggcgtga
     2581 ccatggacat gatgatcctg catggtcagg cggtgatgcg tggttcgcgc aaggccatgg
     2641 tcgtggtcga catgccgttc gggtcctatg aggcctcgcc cgaggtcgcc tatgccaacg
     2701 ccgtgcggat catgaaggaa acgggcgccc aggccgtgaa ggttgaaagc ggtcccacgg
     2761 tgccggcaac gatcgaatat ctgaccaagc gcggcgtgcc ggtcatgggt catgtcggcc
     2821 tgcgtcctca ggccgtgctg gtggacggcg gcttcaaggc caagggcaag gatgaggctg
     2881 gccgagcgct catcctcgaa gaggcccgcc gcaccgccga cgccggcgcc ttcgcggtgg
     2941 tcgtcgaggg cgttgccgaa agcctggccc gcgagatcac cgccgccatc gccccgccga
     3001 ccatcggcat tggcgcctcg gccggctgcg atggtcagat tctggtcacc gacgacatgc
     3061 tgggcctgtt cgactggacg ccgaagtttg tccgtcgcta
//
LOCUS       DAOMEO010000284         3094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3521_length_3094_cov_2.145114, whole genome shotgun sequence.
ACCESSION   DAOMEO010000284 DAOMEO010000000
VERSION     DAOMEO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_3094_cov_2.145114"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLH31_07560"
     CDS             complement(<1..147)
                     /locus_tag="PLH31_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR89109.1"
                     /translation="MSQVPPPIRAVIAPVTPLQQNCTIVWCAKTLKAAVIDPGGEVPR
                     LMKAI"
     gene            complement(251..1879)
                     /locus_tag="PLH31_07565"
     CDS             complement(251..1879)
                     /locus_tag="PLH31_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018080312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR89110.1"
                     /translation="MAGNRLFLKKSIASIQKEAAHSQLKRTLGPINLMSLGIGAIIGA
                     GIFVLTGQVASANAGPAIMLSFIVAGIACGLAGLCYAELASTMPVSGSAYTYAYGTLG
                     EVFAWIMGWLLVLEYGVAASTVAVGWSGYVVSTLHALGVNFPTIMVAGNEAPMWATPL
                     IQAVATPGGGTTFALTGTLNIVAAIGIAMVSALLVLGVSESANVNNAIVVVKVIVLLV
                     FIAVGIQYINPANWSPFIPEPTGQPGEFGVSGIFRGAAIIFFAYVGFEAVSTAAAEAK
                     NPSRDVPIGILGALVVCTLIYMAVAAVMTGVVPYKELASPAPIAVAIDRMALEWADVP
                     YAAAEGGKLNLISFAIKIGAITGLSSVMLVLCYGQTRIFYTMARDGLLPKVFAEIHPK
                     FRTPWLGTILLGVVIAIAASFLPISLLGDLVSLGTAVAFSIVCLSVIYLRIKHPDLPR
                     PFKVPGGIFTAVLGILACLFLAFQNFQPMIVHAMNDNPLPLMILAGYASVGALIYIAY
                     GFWHSKLAKGIDITEDTDLNSPAEAMGRGVDDVK"
     gene            1980..>3094
                     /gene="hrpB"
                     /locus_tag="PLH31_07570"
     CDS             1980..>3094
                     /gene="hrpB"
                     /locus_tag="PLH31_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HQR89111.1"
                     /translation="MRFEGLCLPVMLPIENVLPALKAAMLASHATVLVAPPGAGKTTA
                     VPLALLGEPWVEGRKIIVLEPRRLAARAAATRMASTLGEAVGETVGFRVRLQSKVSAR
                     TRIEVVTEGVFTRMILDDPGLESVAAVLFDEFHERSLDADLGLAFARDVQGLLRDDLR
                     LLVMSATLDGARVSSLLGDAPVIESHGRMFPVDTRHLGRDDRLRLEERVGRAVERALA
                     EETGSLLVFLPGQGEIRRVERWLSERLRRPDVDLAPLYGALEPAVQDRAIAPAAPGRR
                     KVVLATSIAETSLTIQGVRVVIDCGLARVPRYDPASGITRLDTVRVSRAAADQRRGRA
                     GRTEPGVCYCLWDEAETRSLPAFARPEILEADLSRLAL"
BASE COUNT          524 a          974 c         1096 g          500 t
ORIGIN      
        1 gatcgccttc atcaggcgcg gcacctcgcc gcctggatcg atgacggcgg ccttcagcgt
       61 cttggcgcac cagacgatgg tgcagttctg ctgcagcggc gtgaccggcg cgatgacggc
      121 gcggatggga ggcgggactt ggctcacggg atctccttgt caggcccagc ataggcgctg
      181 gcgagcgcgc aagaaagaag gcccgggatc gctcccgggc cttcgatcga tttcgatgga
      241 gaaggtttga ttacttcacg tcgtccacac cgcggcccat ggcctcggcg ggggagttca
      301 ggtcggtgtc ttccgtgatg tcgatgccct tggccagctt cgagtgccag aagccgtagg
      361 cgatgtagat cagcgcgccg acagacgcat agccggccag gatcatcagc ggcagggggt
      421 tgtcgttcat ggcgtgaacg atcatcggct ggaagttctg gaaggccagg aacaggcagg
      481 cgaggatgcc cagaacggcg gtgaagatcc cgcccggcac cttgaacgga cgcggcaggt
      541 cagggtgctt gatgcgcaga tagatcaccg acagacagac gatcgagaag gccacggcgg
      601 tgcccagcga caccagatcg cccagcaggc tgatcgggag gaacgaggcg gcgatggcga
      661 tgacgacgcc cagcaggatc gtgcccagcc acggcgtgcg gaacttgggg tggatctcgg
      721 cgaagacctt cggcagcagg ccgtcgcggg ccatggtgta gaagatccgc gtctggccgt
      781 agcacagcac cagcatgacc gaggacaggc cggtgatcgc gccgatcttg atggcgaagc
      841 tgatcaggtt cagcttgccg ccttcagcgg cggcgtaggg aacatcagcc cattccaggg
      901 ccatgcggtc gatggcgacg gcgatgggag ccgggctggc cagttccttg tagggcacga
      961 cgccggtcat cacggccgcg acggccatgt agatcagggt gcagactacc agcgcgccca
     1021 ggatgccgat cggcacgtcc ctagaggggt tcttggcctc ggcggcggcg gtcgagaccg
     1081 cttcaaaacc gacataggca aagaagatga tcgccgcgcc gcggaagatg ccgctgacac
     1141 cgaactcgcc gggctgaccg gtcggctcgg ggatgaaggg gctccagttg gccggattga
     1201 tgtactgaat gccgacggcg atgaacacca gcagcacgat caccttcacc acgacgatgg
     1261 cgttgttgac gttggccgac tcgctgacgc ccagcaccag cagggccgag accatggcga
     1321 taccgatggc ggccacgatg ttcagggtgc cggtcagggc gaaggtggtg ccgccacccg
     1381 gcgtggcgac cgcctggatc agcggggtgg cccacatcgg ggcctcgttg ccggcgacca
     1441 tgatcgtcgg gaagttcacg ccaagggcat gcagggtgct gacgacgtag cccgaccagc
     1501 cgaccgcgac ggtcgaggcg gcgacgccgt attccagaac cagcagccag cccatgatcc
     1561 aggcgaagac ctcgcccagg gtgccatagg cataggtata ggccgagccc gagaccggca
     1621 tggtcgaggc cagctcagca tagcagaggc cggccaggcc gcaggcgatg ccggcgacga
     1681 tgaacgacag catgatcgcc gggccggcat tggccgaggc cacctggccg gtgagcacga
     1741 agatgcccgc gccgatgatc gcgccgatac ccaggctcat caggttgata ggacccagcg
     1801 tacgtttcag ctggctgtgg gcggcttcct tctggatgct cgcgatcgac ttcttcagga
     1861 acagcctgtt tccggccatg tgtttctttt ccccaacgac cagcgaaccc ccgctagtca
     1921 ggcattgggc cgggacgtta gtcagaccga ggccgtcgcg caacgccttg cggtgacgga
     1981 tgcggtttga gggtctatgc ctccccgtga tgctgccgat cgagaatgtc ctgcccgcat
     2041 tgaaagcggc gatgctcgct tcccatgcga cggtgctggt tgccccgccc ggcgcgggca
     2101 agaccacggc cgtccccttg gccctgttgg gggaaccctg ggtcgagggc cgcaagatca
     2161 tcgtgctgga accccgccgg ctggccgccc gcgctgcggc gacgcgcatg gcctccactt
     2221 tgggcgaggc ggttggtgaa accgtgggct ttcgcgtgcg attgcagtcg aaggtgtcgg
     2281 cccgcacccg catcgaggtg gtgaccgagg gcgtctttac ccggatgatt ctcgatgatc
     2341 cgggactgga gagcgtggcg gcagtcctgt ttgatgaatt tcacgagcgc agcctggacg
     2401 cggaccttgg cctggcgttc gcccgtgacg tccagggact gttgcgcgat gacctgcgcc
     2461 tgctggtgat gtcggccacg ctggacggtg cgagggtatc gtcgcttctg ggcgatgccc
     2521 cggtgatcga aagccatggc cggatgttcc cggtcgatac acgccatctg ggccgcgacg
     2581 atcgcctgcg ccttgaagag cgcgtcggcc gggccgtgga gcgggccctg gccgaggaaa
     2641 ccggcagctt gctggtcttc ctgccgggcc agggcgagat tcgccgggtc gagcgctggc
     2701 tctccgagcg cctgcgccgg cccgatgtgg acctggctcc gctttacggg gctctggaac
     2761 cggccgtcca ggaccgcgcc atcgcgccgg ccgcgccggg acgccgaaag gtggtgctgg
     2821 cgacctccat cgccgagacc agcctgacga tccagggtgt gcgcgtggtg atcgactgcg
     2881 gcctggcccg cgtgccgcgc tacgatccgg ccagcggcat cactcggctc gacaccgtcc
     2941 gcgtcagccg cgccgctgcc gaccagcgcc ggggccgggc agggcgcacc gagcccggcg
     3001 tctgctattg cctgtgggac gaggccgaga cacggtcgct gccggccttc gcgcgtccgg
     3061 aaatcctgga ggccgacctg tcgcgcctgg cgct
//
LOCUS       DAOMEO010000285         3091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3527_length_3091_cov_2.202569, whole genome shotgun sequence.
ACCESSION   DAOMEO010000285 DAOMEO010000000
VERSION     DAOMEO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3527_length_3091_cov_2.202569"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLH31_07575"
     CDS             complement(<1..611)
                     /locus_tag="PLH31_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HQR89112.1"
                     /translation="MIRVSLILFFVAVVAVATLALMGEPGRAQLDWMGWRVEMTAAAA
                     ALLTLFSALAATVHWRGLIWVIEAPRRAARARAESKRQQGVEALSRGFLAVAAGDGSE
                     ARRLAQKAADLAEDAPALVRVLAAQAAETAGDHVAAKAAYNAMLGFPEMRLAGLRGLM
                     QTALSEGDRLAAVRHAETAFGLARTARWAWRALLESRLEAGDWA"
     gene            complement(608..1489)
                     /locus_tag="PLH31_07580"
     CDS             complement(608..1489)
                     /locus_tag="PLH31_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitofilin family membrane protein"
                     /protein_id="HQR89113.1"
                     /translation="MNVAPDAAETVMPSDPALYARRRPLLGPSFWAMIALCVFCVMLG
                     ALVTRFGPTWFPVKPSGDSPVQAAPSVTPPAARAPVGSPIPLEGAAPTVVGADAEIER
                     LETRLALLEAGQKGVLDAAAASLAAAALAEAAQTSGSFEEELASLERVLPMSADLRAL
                     SRLAQTGAPTRAGLAAEFESLAARAATAARNPGEDADLLARVRYALSSIVSIRQVSST
                     RGATPDAKLARAQRLLDEGDVESAIRALDTLPDPARIVLAPWLASAERRVEVDRHVTA
                     IRADALAGLAQVSRAAP"
     gene            complement(1532..2227)
                     /locus_tag="PLH31_07585"
     CDS             complement(1532..2227)
                     /locus_tag="PLH31_07585"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQR89114.1"
                     /translation="MTASGAIWITRARPGAESTARRVEALGFQARVDPLLEIVFLDPV
                     VDLSGIAALAFTSANGVEAFARLTAGRDLPVFAVGETTTRAARAAGFAKPASADGDVE
                     ALAALIARARPGPLLCAGASEPAVDLPARLQAEGLTARALPVYAALDRSPTSETLAAL
                     AGFDAALLHSPRAARGLAAVLESAPAPALRVLCLSQAVAEPLAAALGKGRVGSVAFAS
                     RPRESALLDLLPR"
     assembly_gap    2377..2386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2387..3091)
                     /gene="hemC"
                     /locus_tag="PLH31_07590"
                     /pseudo
     CDS             complement(2387..3091)
                     /gene="hemC"
                     /locus_tag="PLH31_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515445.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
BASE COUNT          471 a         1076 c         1167 g          367 t
ORIGIN      
        1 gcccagtcgc ccgcttcaag ccgggattcc agcaaggcgc gccaggccca gcgcgcggtg
       61 cgcgccaggc caaaggcggt ctcggcatgg cgcacggcgg ccagcctgtc gccttcggaa
      121 agcgccgtct gcatcagacc gcgcagcccc gccagacgca tctcgggaaa gcccagcatg
      181 gcgttgtagg cggccttggc cgcgacatgg tcgccggccg tctcggccgc ctgggcggcg
      241 agcacgcgca ccagggcagg cgcgtcctcg gccagatcgg cggccttctg ggccaggcga
      301 cgcgcctcgg agccatcgcc ggccgccacg gccaggaagc ctcgcgacag cgcttcgacg
      361 ccctgctggc gcttgctctc ggcgcgggcc cgggccgcgc ggcgcggggc ctcgatcacc
      421 cagatcaggc cgcgccagtg caccgtggcc gcgagggcgg aaaacagggt cagcagagcg
      481 gcggcggcgg cggtcatctc gacccgccag cccatccagt ccagttgcgc ccggccaggc
      541 tcccccatca aggccagggt cgccacggcg accaccgcca cgaagaacag aatcagagac
      601 acgcggatca tggcgcggcc cgcgaaacct gggcgagccc ggccagcgca tcggcgcgga
      661 tggccgtgac atggcgatcc acctcgacgc ggcgctcggc cgaggccagc catggggcca
      721 gaacgatccg ggccggatcg ggcagggtgt cgagcgcgcg gatcgcgctc tcgacatcgc
      781 cttcgtcgag caggcgctgg gccctggcca gcttggcgtc gggcgtggcg ccgcgggtcg
      841 agctgacctg gcggatcgag acgatgctgg acagggcgta gcgcacgcgg gccagcaggt
      901 cggcgtcttc gcccggattg cgggcggccg tggccgcgcg cgccgccagg gattcaaact
      961 cggccgccag gcccgcccgg gtcggagcgc cggtctgggc caggcgcgac agggcgcgca
     1021 ggtcggccga catcggcaat acccgctcga gactggcgag ttcttcttcg aacgaccccg
     1081 aggtctgggc ggcctcggcc agggccgccg cggcgagcga agcggcggcc gcgtccagaa
     1141 cgcccttctg gcccgcttcc agcagggcga ggcgcgtttc gaggcgctcg atctcagcgt
     1201 cggcgccaac cacggtcggc gccgcgcctt ccagcgggat gggcgaaccg actggcgctc
     1261 gcgccgccgg cggcgttacg ctgggcgcgg cctgaaccgg gctgtcaccg gaaggcttca
     1321 ccggaaacca ggtcggcccg aaccgggtga ccagcgcgcc gagcatcaca cagaacacgc
     1381 agagggcaat catcgcccag aagctgggcc cgagaagcgg gcggcgacgg gcgtacagcg
     1441 caggatcgct gggcatgacg gtttcagcgg catctggagc gacgttcatg cgccattcgt
     1501 gccccacgcc gcaccgccgc gcaatgtgca gctatcgcgg aaggagatcg agcagggcag
     1561 attcgcgagg acgcgacgca aaggccacag agccgacccg ccccttgccg agcgcggcgg
     1621 ccagcggttc ggcgacggct tgtgacaggc acagaacgcg aagggccggc gcaggcgcgc
     1681 tttccagaac ggccgccaga ccgcgcgccg cgcgggggga gtgcagcagg gcggcgtcga
     1741 aaccggcgag ggcggccagg gtctctgacg tcggcgagcg gtcaagcgcg gcatagacgg
     1801 gcagggcccg cgccgtgagg ccttcggcct gaagacgggc gggaaggtcg acggcgggct
     1861 cgctggcccc ggcgcacaga agcggtcccg gccgcgcccg cgcaatcagc gccgccaggg
     1921 cttcgacatc gccgtcggcg ctggccggct tagcaaaacc cgcggcgcgg gcggcgcggg
     1981 tcgtggtctc gccgaccgcg aagacgggca gatcccgccc ggcggtcaga cgcgcgaagg
     2041 cctcgacacc gttggcgctg gtgaaggcca gggccgcgat cccgctcagg tcgacgaccg
     2101 gatcaagaaa tacgatctcc agcagggggt cgacacgcgc ctgaaagccc agcgcctcga
     2161 cgcggcgggc ggtggactcg gcgccgggac gggcgcgggt gatccagatc gccccgctgg
     2221 cggtcatcgt ctagtccggc aggatcaggg cgtcgccgcc ctcggccttg acggccttga
     2281 gcccaagcga gccgtggctg ccggtgccgg acaggatgat gcagatggcg cgttcctgct
     2341 ggtccgctgc cagtgagcgc agaaagggat cgatcgnnnn nnnnnnccca gcgacaggcc
     2401 cagatcgcgg gcggcggtga cgtcgtccgg tccgtccagc gtcacctggc cctcgcggcg
     2461 gaagcgctga acgccgtcgg gcgtcagggc ctcgacgatc agcgacaggc gctggccgtc
     2521 cagggtggcg taggcgccga tggccgtgcg gcacgagccc tcgagcgcgt agagcgcccc
     2581 gcgctcggcg gcgacacaga ccgtggtcgg gcggcagcgg acggcctcaa gccagtcggc
     2641 gttcacatct tcaaggcgcg tctcgatcac gagggcgccc tggcccgggg ccggcgggca
     2701 ggcgatgggg tccagccagc tcttgggcag atggcccaga cccagacggt tcaggcccga
     2761 ctgggccagc aggatggcgt cggcctcgcc gcgttccagc ttggccaggc gggtgtcgac
     2821 attgccgcgc agcatgacga tctgcagatc gggccgcaca tgcagggcct gggcctggcg
     2881 acgcggtgcc caggcgcgcg ccgtggggca ggtcttccag tcgatcgcag agggtgctga
     2941 tgaaggcgtc gcggggatcc tcgcgctcgg gcgtggcggc caggatcagg cccggcggca
     3001 gctcggccgg catgtccttc agcgagtgga tggcgcagtc gatacggccg tccagcaggg
     3061 cttcctcaat ctccttggtg aacaggccct t
//
LOCUS       DAOMEO010000286         3083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3534_length_3083_cov_2.478864, whole genome shotgun sequence.
ACCESSION   DAOMEO010000286 DAOMEO010000000
VERSION     DAOMEO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3534_length_3083_cov_2.478864"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>756
                     /locus_tag="PLH31_07595"
     CDS             <1..>756
                     /locus_tag="PLH31_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR89115.1"
                     /translation="AQVASLEAELQVARRSLLILTGRGVDPLASLTVPAALPDAPLTP
                     AAVPGALLARRPDVREAQARLASASANLKVNELALFPTFTLAPGLGLTRAVSPSFFTP
                     GTSTSTTSSAWSIGLNLSIPVLNIPKLMADIDAQDARTEQAAVTYEKTVQTAFGEAEG
                     ALLQLSADQRRVALLTAGERRAATAYEAYRRGYAAGLSDLTSALQAEQTWRAARTALS
                     GARGQALQRAVQTYKALGGGWSPQAVPETASSR"
     assembly_gap    757..766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            778..>1301
                     /locus_tag="PLH31_07600"
     CDS             778..>1301
                     /locus_tag="PLH31_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR89116.1"
                     /translation="MNRPLIAILFLTTSLGLAACGEKKDQPAASAASAPARAVTVGRV
                     ESRPLGAGFEASGQLVPREEAAVGSELAGYRVARVYVEEGAYVRAGQPLVQLDDTLLR
                     AQIAQQAAVTAQAQAEAARVAGLDGQGVLSQEQIESRRYAAKAQEAALAELRTRSSRM
                     TITAPVGGRVLERAV"
     assembly_gap    1302..1311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1312..1850
                     /locus_tag="PLH31_07605"
     CDS             <1312..1850
                     /locus_tag="PLH31_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR89117.1"
                     /translation="VRPGEISGGATAPWFRIARDGLVELDAEVGDAQLSSLSVGQSAQ
                     VALPDGRVVSGVVRLVSPRIDAATRLGKVRVRLPNDAGLRPGGFGRATFAPSGRKVKA
                     APETAIRYDAEGSSLVTVDNSNRARQTPVRTGQRGGGWVELLDGPPVGARVLLGGSAF
                     TLDGDLVKPTDAAGKAAR"
     gene            1850..>2839
                     /locus_tag="PLH31_07610"
     CDS             1850..>2839
                     /locus_tag="PLH31_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89118.1"
                     /translation="MAGVRISAWSIKNPIPVAVLFIALILAGIASYMMLPIKQYPNVS
                     FPLVAVTITQSGAAAGEMETQITRQVEDAVAGISNVKSIRSSVVQGVSTTSIEFDIGE
                     DLQKATDEVRSKIDQSRALLPREIDEPIVQRVDLDSLPIITYAVSSPSMTISELSWFV
                     DDTISRRLQGEKGVAQVARVGGVDREINIVIDPDRMAASGLTAPQLNAALASFSLDAP
                     GGRLAIGAREQTVRVLAAARTVEQLRALTIPTTGGRFVRLTDVAEVGDGAGEARSFAR
                     LDGRPVVAFQVSKTKDASDVRVEDGIKIAIDKLVEAHPDVTFTKIYSIVDDTRA"
     assembly_gap    2840..2849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2850..>3083
                     /locus_tag="PLH31_07615"
     CDS             <2850..>3083
                     /locus_tag="PLH31_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89119.1"
                     /translation="LEGMLLAALVVFLFLREWRATLITAIAMPMSLIPTFIFMAVMGF
                     SLNVVTLLGLTLVIGILVDDAIVEIENIEKRVAA"
BASE COUNT          455 a         1127 c          989 g          482 t
ORIGIN      
        1 ggcccaggtc gccagtctgg aagccgaact gcaggtcgcg cgccgctcgc tgctgatcct
       61 gactggacgc ggcgtcgatc ccctggccag cctgactgtc ccggctgcgc tgcccgatgc
      121 gccgctgacg cccgccgccg tgcccggcgc gctgctggct cgccgccccg atgtgcgcga
      181 agcccaggcg cgcctggcct cggcctcggc caatctcaag gtcaacgagc tggcgctctt
      241 cccgaccttc accctggccc ccggtcttgg cctgacccgg gcggtgtcgc ccagtttctt
      301 cacgccggga acctcgacct cgaccaccag ttcggcgtgg tcgatcgggc tgaacctgtc
      361 gattccggta ctgaacatcc ccaagctgat ggccgatatc gacgcccagg acgcccgcac
      421 cgagcaggct gcggtcacct atgaaaagac cgtccagacc gcgttcggcg aagccgaggg
      481 ggccctgctc cagctatccg ccgaccagcg tcgcgtcgcc ctgctcaccg ccggcgagcg
      541 ccgggccgcc acggcctatg aagcctatcg ccggggctat gccgctggcc tcagcgacct
      601 gacctcggcc ctgcaggccg aacagacctg gcgcgccgcc cgcaccgccc tcagcggcgc
      661 gcgaggccag gccctgcaac gggccgtgca gacctacaag gcgcttggcg gcggctggtc
      721 gccccaagct gttcccgaaa ccgcctcctc gcgatgnnnn nnnnnngatc ccccacgatg
      781 aaccgccccc tgatcgccat cctctttctg acgaccagcc tgggcctcgc cgcctgcggt
      841 gaaaagaagg atcagccggc cgcctcagcc gcctcagctc cggcgcgcgc tgtcaccgtc
      901 ggccgtgtgg aaagccgccc gctgggggcc gggttcgaag cctcgggcca gctggttccg
      961 cgcgaggaag ccgccgtagg ctccgagctg gcgggctatc gcgtcgccag ggtttatgtc
     1021 gaggaaggcg cctatgtccg cgctggccag cctctcgtcc agctggacga caccctgctg
     1081 cgtgcccaga tcgcccagca ggcggccgtc accgcccagg cccaggccga agccgcgcgg
     1141 gtcgccgggc tggatggcca gggcgttctc agccaggaac agatcgaaag ccgccgttac
     1201 gccgccaagg cccaggaagc ggccctggcc gaactgcgca cgcgctcaag ccggatgacc
     1261 atcacggccc cggtcggcgg acgggtgctg gaacgcgccg tnnnnnnnnn nccgtacggc
     1321 ccggcgagat ctcgggtggc gcgaccgcgc cctggttccg catcgcccgc gacggtctgg
     1381 tcgaactgga cgccgaggtg ggcgacgcac agctttcctc gctctcggtc ggacaatcgg
     1441 cgcaggtcgc ccttcccgac ggccgcgtgg tgtctggcgt ggtgcgtctg gtcagtccgc
     1501 ggatcgacgc cgccacgcgc ctgggcaagg tccgcgtcag actgcccaac gatgcaggcc
     1561 tgcgccccgg cggctttggt cgcgccacgt tcgccccctc gggccgcaag gtgaaggccg
     1621 cgccggaaac cgccattcgc tatgacgccg agggctcgtc cctggtgacg gtcgacaatt
     1681 ccaaccgcgc ccgccagacc ccggttcgca ccggtcaacg cggcggcggc tgggtggaac
     1741 tgctcgacgg cccgcccgtc ggcgcgcgcg ttctgctggg cggctcggcg ttcactctgg
     1801 acggcgatct ggtcaagccc accgacgccg ccggcaaggc tgctcgctga tggctggcgt
     1861 ccgcatatcc gcctggtcga tcaagaaccc gatcccggtc gcggtcctgt tcatcgccct
     1921 gatcctggcc ggcatcgcca gctacatgat gctgcccatc aagcagtatc ccaatgtctc
     1981 gttcccgctg gtggcggtga ccatcaccca gagcggcgcg gccgccgggg aaatggaaac
     2041 ccagatcacc cgccaggtgg aagacgccgt agcgggcatc tccaacgtca agagcatccg
     2101 ctcgtcggtc gtccagggtg tttccaccac ctcgatcgaa ttcgatatcg gcgaggacct
     2161 gcagaaggcc accgacgagg tgcgctccaa gatcgaccag tcgcgcgccc tgctgccgcg
     2221 cgagatcgat gagccgatcg tccagcgcgt cgatctggat tccctgccga tcatcaccta
     2281 cgccgtctcg tcgccctcca tgacgatcag cgagctgtcc tggttcgtgg acgacacgat
     2341 cagtcgccgc ctgcagggtg aaaagggcgt ggcccaggtg gcccgggtcg gcggcgtgga
     2401 tcgcgagatc aacatcgtca ttgaccccga ccgaatggcg gcctcgggcc tgaccgcgcc
     2461 gcagctgaac gccgccctgg ccagtttcag cctcgacgcc cccggcgggc ggctggcgat
     2521 cggcgcgcgc gagcagaccg tccgcgttct ggccgctgcc cgcaccgtcg agcaactgcg
     2581 cgccctgacc attcccacta ccggcggtcg cttcgtccgc ctgaccgacg tggccgaggt
     2641 cggcgacggg gctggcgagg cccgctcgtt cgcgcgcctg gacggccgcc ccgtggtcgc
     2701 cttccaggtc agcaagacta aggacgccag cgatgtgcgg gtcgaggacg gcatcaagat
     2761 cgccatcgac aagctggtcg aggcgcatcc cgacgtcacc ttcaccaaga tctattcgat
     2821 cgtcgacgac acccgcgccn nnnnnnnnng ctggaaggca tgctgctggc ggccctggtg
     2881 gtgttcctgt tcctgcgcga atggcgggcc accctgatca ccgccatcgc catgccgatg
     2941 tcgctgatcc cgaccttcat cttcatggcg gtgatgggct tttcgctcaa tgtcgtgaca
     3001 ctgctgggcc tgaccctggt gatcggcatc ctggtcgatg acgccattgt cgagatcgag
     3061 aatatcgaga aacgcgtcgc cgc
//
LOCUS       DAOMEO010000287         3080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3540_length_3080_cov_2.626777, whole genome shotgun sequence.
ACCESSION   DAOMEO010000287 DAOMEO010000000
VERSION     DAOMEO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_3080_cov_2.626777"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    223..232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            544..1428
                     /gene="dapA"
                     /locus_tag="PLH31_07620"
     CDS             544..1428
                     /gene="dapA"
                     /locus_tag="PLH31_07620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQR89120.1"
                     /translation="MVHSPFKGVIPALITPFRDGAVDEEAFVALVERQIAGGVHGVVP
                     VGTTGETSTLSHDEHKRVVELCVQTVKGRVPVIAGAGSNSTDEAIELAQHAKAVGADA
                     ALVVTPYYNRPSQEGLYRHYEAINDAVQLPVLVYNVPSRASVDIANETLARLSKLPNI
                     IGVKDATGDLARASMQRVLCGPDWVMLSGDDPSALGYMAHGGHGVISVTANVAPEACS
                     AFINACMQGAWETALYGQDRLIKLHRALFLDSSPAPTKFAMAHLGLCQPDVRLPIAPC
                     NDAVKPAILEAMREAGLV"
     gene            1428..1886
                     /gene="smpB"
                     /locus_tag="PLH31_07625"
     CDS             1428..1886
                     /gene="smpB"
                     /locus_tag="PLH31_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQR89121.1"
                     /translation="MTKPIAENRRARYDYFIEETFEAGIMLTGTEVKSLRVGRANIAE
                     SYASVEGRQIKLINADIPPYGHANRFNHEPRRHRTLLLHKKQIEKLIGAVQRDGRTLI
                     PLKLYWNEKGLAKLEIGLAKGKKNHDKRETEAARDWQRDKARLMKGDRGD"
     gene            complement(1901..2926)
                     /locus_tag="PLH31_07630"
     CDS             complement(1901..2926)
                     /locus_tag="PLH31_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HQR89122.1"
                     /translation="MSTPSGSSSPSLARSTASRFARIALGHVAREYPNKLDHVMAGDA
                     DARTPRDLHPIFFGSFDWHSCVHGYWLLATLLRIRPDMAEAETIIALFDEAFTDAKVA
                     GEVDYLARPESRGFERPYGWAWDLMLQAELLRHRDRPWAARHAPLARAFVERFEAFLP
                     LADYPVRAGVHTNTAFALVLASEYADMTGNAVFRQLLTTKALLWYGQDAACQAWEPSG
                     DDFLSPALIEAEAMRRLTPRQTFEIWFVRFLPDLASKQPATLFTPARVSDRTDGKIAH
                     LDGLNLSRAWCWRTLASALPELDPAKPLAVNAAMAHLTAAVPHISSDYMGEHWLATFA
                     LLALTND"
BASE COUNT          560 a          985 c         1043 g          482 t
ORIGIN      
        1 gtcgaccaga gcccaaaggg cgatcttgcg ggcgacggga tcctgcaggc tgctcatcgc
       61 catgcgggcg cgggagacat cgccgctctt ggcggcggcg agcgccatac gcagattttc
      121 ggcttcgacg tcggagacgg ttccagggat cgcggtcggc ggagtcgtca ccagggatgg
      181 ggtcgacgag ggttggtagg gaacaggccc agacgtctgg gcnnnnnnnn nnccgcgacg
      241 ctgccaagca gcaccaggcc ggcgcttccg aacatgatcc gacgcaacac tgaagccaaa
      301 acccactctc ctcaacgcac tgccgaatcg agccgacagc cgcccatgcg atccatcagg
      361 ccccgagaca cctaaggaac ggtttttcta aggcctcaag ggcttgaccc tcggggcttg
      421 gcccatcaaa ccgctgcctg ctccgacgcg cccgttgcgg aacggcgggg cggggacata
      481 ttgtccgccc cgatcgtatg gccgcaacct tcgcggcgcc tttctcacgg caaattgcaa
      541 gttatggtcc attctccgtt caagggcgtc attcccgccc tgattacgcc ctttcgcgac
      601 ggcgccgttg acgaggaagc ctttgtggcc ctcgtcgagc ggcagatcgc gggcggtgtt
      661 cacggtgtcg tgccggtcgg caccaccggc gaaacctcga ccctgagcca cgacgagcac
      721 aagcgcgtgg tcgagctgtg cgttcagaca gtcaaggggc gggtgcctgt gatcgccggc
      781 gcaggttcaa actcgactga cgaagccatc gagctggccc agcatgccaa ggcggtcggc
      841 gccgacgccg cgctggtggt gacgccctat tataatcgtc cgagccagga aggcctgtac
      901 cggcattatg aggcgatcaa cgacgccgtc cagttgccgg ttctggtcta taacgtcccc
      961 agccgggcca gcgtcgacat cgccaacgag accctggcgc gcctctccaa gctgcccaat
     1021 atcattggcg tcaaggacgc caccggtgat ctggcccgcg ccagcatgca gcgcgtcctg
     1081 tgcggtcccg actgggtgat gctttcgggc gatgatccct cggcgctggg ctacatggcc
     1141 catggcggcc atggcgtgat ctcggtgacc gccaacgtcg cccccgaggc ctgctcggcc
     1201 ttcatcaacg cctgcatgca aggcgcctgg gaaaccgcgc tctatggcca ggaccgcctg
     1261 atcaagctgc atcgggccct gttccttgac tcctcgccgg ccccgaccaa gttcgccatg
     1321 gcccatctgg gcctgtgcca gccggacgtg cgtctgccga tcgcgccctg caacgacgcc
     1381 gtgaagccgg cgatcctgga ggccatgcgc gaggcgggtc tggtctgatg accaagccga
     1441 tcgcggagaa ccggcgcgcg cggtacgact acttcatcga ggaaaccttc gaggccggca
     1501 tcatgctgac cggcaccgag gtgaagagcc tccgcgtcgg tcgcgccaat atcgccgagt
     1561 cctatgcgtc ggtcgaaggt cgtcagatca agctgatcaa cgccgacatc ccgccctatg
     1621 gccatgccaa ccgcttcaat cacgagccgc gccggcaccg gacgctgctg ctgcacaaga
     1681 agcagatcga gaagctgatc ggcgccgtgc agcgcgacgg ccgcacgctc attccgctga
     1741 agctctattg gaacgagaaa ggcctggcca agctggagat cggcctggcc aagggcaaga
     1801 agaaccatga caagcgcgaa accgaggccg cccgcgactg gcagcgcgac aaggcccgcc
     1861 tgatgaaggg cgaccggggg gactaaagcc ctccttcggc ctagtcgttt gtcagggcca
     1921 gcagggcgaa ggtcgccagc cagtgctcgc ccatgtagtc gctcgatatg tgcggcacgg
     1981 cggcggtcag atgcgccatg gcggcgttga cggccagggg cttggccgga tccagctccg
     2041 gcagggcgct ggccagagtg cgccagcacc aggcgcgaga caggttcagg ccgtccaggt
     2101 gagcgatctt gccgtcggtg cgatcactga cgcgggcggg ggtgaacagg gtcgctggct
     2161 gcttgctggc aaggtcaggc aggaagcgaa cgaaccagat ctcgaacgtc tgtcgcgggg
     2221 tcagccggcg catggcctcg gcctcgatca gggcagggga cagaaagtcg tcgccggagg
     2281 gctcccaggc ctggcaggct gcgtcctggc cgtaccagag gagcgctttg gtggtgagaa
     2341 gctggcggaa cacggcgttg ccggtcatgt cggcatattc cgaggccagc accagggcga
     2401 aggcggtgtt ggtgtggacg cccgcgcgga ccggataatc ggccagcggc aggaaggcct
     2461 cgaaccgttc gacaaaggcg cgggcgaggg gggcgtgccg cgccgcccag gggcgatccc
     2521 ggtggcgcag caactcggct tgcagcatca ggtcccaggc ccagccatag ggacgttcga
     2581 acccccggct ctcaggacgg gccagatagt cgacctcgcc ggcgaccttg gcgtctgtga
     2641 aggcctcgtc gaacagggcg atgatcgttt cggcctcggc catatcgggc cggatgcgca
     2701 gcagggtcgc cagcaaccag tagccgtgga cgcaggagtg ccaatcgaag ctgccgaaga
     2761 agatgggatg caggtcgcga ggcgtgcggg cgtcggcgtc gccggccatc acgtggtcca
     2821 gcttgttggg atactcgcgc gcgacatggc ccagcgcgat cctggcgaac cgcgaagccg
     2881 tcgagcgcgc caggctgggt gagctagacc ccgaaggcgt ggacattcat caggaccaca
     2941 ttgacgaccg gcatgaccac gatggtggga ccggggcgcg gatgacgccc ttatggtgct
     3001 tcaatggcaa caaggccgcc gacgcaagat tgaagttcgc cgccgcgtgt cttcgcaacg
     3061 ggtattgagg gcgctggcca
//
LOCUS       DAOMEO010000288         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3551_length_3070_cov_2.892206, whole genome shotgun sequence.
ACCESSION   DAOMEO010000288 DAOMEO010000000
VERSION     DAOMEO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_3070_cov_2.892206"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..990
                     /locus_tag="PLH31_07635"
     CDS             100..990
                     /locus_tag="PLH31_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQR89123.1"
                     /translation="MIDLVIEARRHDIGNFEVGRVLPFHAHRMVGPFTFLDHMGPAEF
                     APGFPKSADVRPHPHIGLSTLTYLFQGEITHRDSVGSLAVIKPGEVNWMTAGSGITHS
                     ERFETLRRQGGRMDGMQAWIALPQESEEVDPSFSHHEGDAALPSYESGGLKARLIAGE
                     AFGAKTNVPVYSPLFYVHWEMAAGTTAALPAEYSERAAYVASGRVEVDGRELTGLQMA
                     VFAPGDTIVFKALEPSTVMLLGGEPVGPRFMEWNFVSSSKDRLEQAKADWKAGRMKLP
                     DLDHDEFIPFPEKPASPQPL"
     gene            complement(1021..2094)
                     /locus_tag="PLH31_07640"
     CDS             complement(1021..2094)
                     /locus_tag="PLH31_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQR89124.1"
                     /translation="MTVSQKLLPGVPIVESMLFSASLDAMGLTNSERDIAIQLNEQGF
                     AVLDFPDEDLDQRIDRIVTALTPQYDFDQWRTHGWAANTGLRVQDAWKTHDDVRAIAA
                     NAAVLDLLGKLYGRRAFPFQTLNFPVGTQQHIHSDSIHFSSVPERFMCGVWLALEDIH
                     PDAGPLVYYPGSHKWPIIYNDMIGKVIGAGADFLAQSPYENAWAAMVEATGAKPEYFY
                     PRKGQALIWAANLLHGGSRQNDPGRTRWSQVTHYYFDDCAYFTPAFSDPLVGNLDLRQ
                     IVDITTGELAPNIYVDRPVDQVVRRGAPPAPVAQSAPVPGKLPKDFDPALYLSLNPDV
                     AAAGADARKHYLRFGMRENRRYR"
     gene            2303..2728
                     /locus_tag="PLH31_07645"
     CDS             2303..2728
                     /locus_tag="PLH31_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89125.1"
                     /translation="MSYREKLAWLSLVAMAVTYGPYFTLAALAPPTTALPDLSRLGVF
                     AATALSHMVIILVGRLYFMARSPEDARAPADERDLAIERRSKTTAYFLLMAGLIMVGV
                     VMPFTAGGWVIVNAALATIVGVEILRDGLIVLGYRRSMA"
     gene            2725..2943
                     /locus_tag="PLH31_07650"
     CDS             2725..2943
                     /locus_tag="PLH31_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89126.1"
                     /translation="MSPGTIANQIRTLRFLHGEMSQAELGQRIGLTRQTVAAIEAGKY
                     SPSLEAAFRIAEVFGKPLDEVFQWVKTA"
BASE COUNT          541 a         1027 c          969 g          533 t
ORIGIN      
        1 gtgatgggca tcgccattcc gcgctgacac gacgcatttc gacaagacgc ccaccgcaac
       61 tcatgagagt ctccggccat ccccgcgccg gagtcctcca tgatcgacct cgttatcgaa
      121 gcccgccgtc acgacatcgg caatttcgag gtcgggcgcg tcctgccatt ccacgcccat
      181 cgcatggtcg gccccttcac cttcctcgac cacatgggcc ccgccgaatt cgccccgggc
      241 tttcccaaga gcgccgatgt gcgccctcac ccgcatatcg gcctgtccac actgacctat
      301 ctgttccagg gcgagatcac ccatcgcgac agcgtcggct cgctggcggt gatcaagccg
      361 ggcgaggtca actggatgac cgccgggtcg ggcatcaccc attccgagcg cttcgagacc
      421 ctgcgccgtc agggcggccg gatggacggt atgcaggcct ggatcgccct gccccaggaa
      481 tccgaggaag tggatcccag cttcagccac catgagggcg acgccgccct gccgtcctat
      541 gagagcggcg ggctgaaggc gcggctgatc gccggcgagg cgtttggcgc caagaccaac
      601 gtgccggtct attcgccgct cttctatgtg cactgggaaa tggccgccgg aaccaccgcc
      661 gccctgccgg ccgaatattc cgagcgcgcc gcctatgtcg ccagtggtcg cgtcgaggtc
      721 gacggccgcg aactgaccgg cctgcagatg gctgtgttcg cacccggcga caccatcgtc
      781 ttcaaggccc tggagccctc caccgtcatg ctgctgggcg gagagcccgt gggcccgcgc
      841 ttcatggagt ggaacttcgt ctcgtcctcg aaagaccggc tggagcaggc caaggccgac
      901 tggaaggccg gccgcatgaa gctgcccgac ctcgaccatg acgagttcat tccgttcccg
      961 gaaaaaccag cgtcaccgca accgctgtag cggccctgct gcgccgaaac cggcagaccg
     1021 tcagcggtac cggcggttct cgcgcatgcc gaaccggaga tagtgcttcc tggcgtcggc
     1081 gccggccgcc gcgacatccg gattgagcga cagatagagc gccgggtcga agtccttcgg
     1141 caacttgccg ggaacgggcg cggactgggc gacaggagcg ggcggagcgc cccggcgcac
     1201 gacctgatcg accgggcggt ccacatagat gttgggcgcc agctcaccgg tcgtaatgtc
     1261 gacgatctgg cgcaggtcca aattgccgac cagcgggtcg gaaaaggcgg gcgtgaaata
     1321 ggcgcagtcg tcgaaatagt agtgcgtcac ctgcgaccag cgcgtccgcc ccgggtcgtt
     1381 ctggcggctg ccgccgtgca gcaggttggc cgcccagatc agcgcctggc ccttcctggg
     1441 atagaagtat tccggcttgg cccccgtggc ttccaccatc gccgcccagg cgttttcgta
     1501 agggctctgg gccaggaagt cggcccccgc gccgatcacc ttgccgatca tgtcgttgta
     1561 gataatcggc cacttgtggg agcccggata atagaccagc gggccagcgt cgggatggat
     1621 atcctcaagg gccagccaga caccgcacat gaaccgctcg ggcacgctgg aaaagtggat
     1681 cgagtccgag tggatatgtt gctgggttcc gaccgggaag ttcagcgtct ggaacggaaa
     1741 ggcccgccgg ccatacagct tgcccagcag atccagcacg gcggcgttgg cggcgatggc
     1801 gcgcacgtcg tcatgggttt tccaggcatc ctgcacccga aggccggtat tggcggccca
     1861 gccgtgggtt cgccactggt cgaagtcgta ctgcggcgtc agcgctgtga cgatgcggtc
     1921 gatccgctgg tcaagatcct catcgggaaa gtccagaacg gcgaagccct gctcattgag
     1981 ctgaatggcg atgtcgcgct cactattggt cagccccatc gcgtcgagtg aggccgaaaa
     2041 cagcatggac tcgacgatcg gcacgccggg caaaagcttc tgggaaacag tcatcggccc
     2101 ttgcgactcc taccttgtac ataagctgac ttaaccggcg tggagggcca tttccagcca
     2161 cagagcggcc gcaggcgctg aaaccctcgc catccagggg cgtcgctcat gcccccctac
     2221 aggcgagagt tggaaatcat agacatcgtg ttatgtttgc tttacatcga gtaaaaattt
     2281 atactcaccc ccggagcgac tgatgtccta tcgtgagaaa ctcgcctggc tttccctggt
     2341 cgccatggcc gtcacctatg gcccctattt caccctggcg gcgctggccc cgccgacaac
     2401 cgccctgccc gatctcagcc ggctgggcgt gttcgccgcg accgcgctga gccacatggt
     2461 catcatcctg gtcggacgcc tgtatttcat ggcccgctcg cctgaggacg cccgggcgcc
     2521 ggccgatgag cgcgacctgg ccatcgagcg gcggtccaag accaccgcct atttcctgct
     2581 gatggccggc ctgatcatgg tgggcgtggt gatgcccttc acggccggcg ggtgggtcat
     2641 cgtcaacgcc gccctggcca ccatcgtcgg cgtggagatc ctgcgcgacg gcctcatcgt
     2701 gctcggctac cgccggagca tggcatgagt ccggggacca tcgccaacca gatccgcacc
     2761 ctgcgcttcc tgcacggcga gatgtcccag gccgagcttg gccagcgcat cggcctcacc
     2821 cgccagacgg tcgcggccat cgaggcgggc aagtactcgc cgtcgctgga agcggccttc
     2881 cgcatcgccg aggtgttcgg caagccgctg gacgaggtat tccagtgggt gaagaccgcc
     2941 tgactacttg gccggggtga accggcggat cgggcgcagc accggctcga ggatggcatt
     3001 gatcgcctgc cacaccgccg aaaccagatt gttgtgcagg ttgaccacgt tgaagctgat
     3061 caacaggctc
//
LOCUS       DAOMEO010000289         3069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3553_length_3069_cov_2.515926, whole genome shotgun sequence.
ACCESSION   DAOMEO010000289 DAOMEO010000000
VERSION     DAOMEO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_3069_cov_2.515926"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLH31_07655"
     CDS             <1..612
                     /locus_tag="PLH31_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQR89127.1"
                     /translation="EAPGPLEAILPAGAHGRAPDLRAWAAFRDETGLPILVEAVEAFD
                     VIQSAPVPVIVGLPSGQGVFLACEDATPIVAMEAPLFEGEAGLDLWPQKRPRLATAAQ
                     RLRVLLLDAPIGFQPGWGMSWVSETCALSLPDDTAPAVLTKLAEAGVEAVGASFRARD
                     MEDDETPVADRLASSVVVIRLDPDLGLQALDDIAAALRSAVAG"
     gene            complement(753..1787)
                     /locus_tag="PLH31_07660"
     CDS             complement(753..1787)
                     /locus_tag="PLH31_07660"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HQR89128.1"
                     /translation="MTDTDLPQADLATFVRGLPKAELHMHIEGSLEPELMFELARRNG
                     VTLPYGSVEEIRAAYAFSNLQDFLDIYYQGAGVLITEEDFKDLALAYFRRLAADGGTH
                     AEIFFDPQTHTDRGIAFDVVMKGLLAGMDEAEKTLGVTSKLILCFLRHLDEDAAFATL
                     EQAKPWLSKIAGVGLDSSEVGHPPAKFARVFQAARDLGLKIVAHAGEEGPPDYVWEAI
                     DLIKVDRIDHGNRALEDEALTARLVRDGVTLTVCPLSNLKLCGVKTLEEHPLKRMLDL
                     GLKATVNSDDPAYFGGYLLENYMATATAVGLTRDELVTLAKNSFSGSFLSDAEKAERV
                     AGVEAYAAAN"
     gene            1908..>3069
                     /locus_tag="PLH31_07665"
     CDS             1908..>3069
                     /locus_tag="PLH31_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HQR89129.1"
                     /translation="MSTPFLRSLALLAALSAAVPAAAEEAPATERWWAHVKVLADDNM
                     EGRLAGSPGHARAAAYVAERFAAYGLKPAGTEGYLQPVPLVLQRVFPDKSSVALVKDG
                     VAMSLVLGEDLTLPARIPQPASVPDAPLVFIGYGLHMPEAGHDDFAGVDLRGKIAVVL
                     SGGPSAVGAPLRAHAARERWEALQKAGAVGLVSIQNPKQVEIPWERARLRAVEPAMRL
                     ADPALNPVKGELFTAVMNPAYANKLFAPSGRTLEELLVLAEAGKALPGFALNQSLRAT
                     VATETTQAVSSNVAALLPGSDPVLSKDYVVLSAHLDGLGVGQPIDGDAINNGAMDNAS
                     GVASLLEQAAALKTGAPPKRSILFVAVTAEEQGLLGSQYFAERPTVPKAALVA"
BASE COUNT          446 a         1062 c         1053 g          508 t
ORIGIN      
        1 gaagcccccg gtccgctgga agccatcctg cccgccggcg ctcacggccg cgcgcccgac
       61 ctgcgcgcct gggccgcctt ccgcgacgag accggcctgc cgatcctggt cgaggccgtc
      121 gaggccttcg acgtcatcca gagcgcgccg gtgccggtca ttgtcggcct gcccagcggt
      181 cagggcgtgt tcctcgcctg cgaggacgcc accccgatcg tcgccatgga agccccgctg
      241 ttcgaaggcg aggcgggcct ggacctgtgg ccgcagaagc gcccgcgcct ggccaccgcc
      301 gcccagcgcc tgcgggtttt gctgctggac gcccccatcg gcttccagcc gggctggggc
      361 atgagctggg tctccgagac ctgcgccctc agcctgcccg acgacaccgc gccggccgtg
      421 ctgaccaagc tggccgaagc cggcgtcgaa gccgtcggcg cttctttccg cgcccgcgac
      481 atggaagacg acgagacgcc ggtggcggat cgtctggcca gttcggtcgt cgtgatccgc
      541 cttgatccgg accttggcct gcaggcgctc gatgatatcg cggcggcgct gcggagcgcc
      601 gtggcgggat aattcagaac ccttccccct tgatggggga agggcagggt tgggggtgaa
      661 gcggcggtgg gtttcattcc gaccgcgtca atatcggaaa cggtgtcacc cccatcccca
      721 acccttcccc catcagaggg ggaaggggga aatcaattcg ccgccgcgta agcctcgacg
      781 cccgcgacgc gctcggcctt ttcggcatcg ctcaggaacg accccgagaa gctgttcttg
      841 gccagggtca ccaactcgtc gcgcgtcagg cccaccgccg tcgccgtagc catgtagttt
      901 tccagcagat agccgccgaa ataggccggg tcgtcggaat tgaccgtggc cttgaggccc
      961 aggtccagca tgcgctttag ggggtgctcc tccagcgtct tcacgccgca cagcttcagg
     1021 ttcgacaggg ggcataccgt cagggtcacg ccatcgcgca ccagccgcgc ggtcagggcc
     1081 tcgtcctcca gcgcccggtt gccgtggtcg atgcggtcga ccttgatcag gtcgatggct
     1141 tcccagacat agtccggcgg gccttcctcg ccggcgtgag cgacgatctt gaggcccagg
     1201 tcgcgggccg cctggaacac ccgggcgaac tttgcgggcg ggtggccgac ctcggagctg
     1261 tcgaggccaa cgccggcgat cttcgacagc cacggcttgg cctgttccag ggtggcgaag
     1321 gcggcgtcct cgtccagatg gcgcaggaag cacaggatca gcttggaggt gacgcccagc
     1381 gttttctcgg cttcatccat gccggccagc agacccttca tcaccacgtc gaaggcgatc
     1441 ccgcgatcgg tgtgggtctg ggggtcgaag aagatctcgg catgggtgcc gccatcggcc
     1501 gccaggcgcc ggaaataggc cagggccagg tccttgaagt cttcctcggt gatcagcacc
     1561 ccggcgccct ggtaatagat gtccaggaag tcctgcaggt tggagaaggc gtaggccgcg
     1621 cggatctctt ccaccgagcc atagggcagg gtgacgccgt tgcgccgggc cagctcgaac
     1681 atcagctcgg gctcgagact gccctcgatg tgcatgtgca gttcggcctt gggcaggccg
     1741 cggacgaagg tggccaaatc agcctgggga agatcggtgt cggtcatgga gacgttatgg
     1801 gcgttccggc ccgcgccttt caaccccgcc tggcgcgatc cggcgtcgtg agcgatcttc
     1861 gcgctttccc gttgcgtctg tcgcctccgg atttaacaat ccccgccatg agcacccctt
     1921 tccttcgcag ccttgcgctg ctggccgccc tgtccgccgc cgtccccgcc gccgccgagg
     1981 aggcgcccgc caccgaacgc tggtgggcgc atgtgaaggt tctggccgac gacaatatgg
     2041 aagggcgcct ggcgggcagc ccgggccatg cgcgcgccgc cgcctatgtg gccgagcgtt
     2101 tcgccgccta tggcctgaag cccgccggga ccgagggtta tctccagccg gtgccgctgg
     2161 tgctgcagcg ggtctttccc gacaagtcca gcgtcgccct ggtcaaggac ggcgtcgcca
     2221 tgtcgctggt gctgggcgag gacctgacgc tgccagcccg catcccccag ccggccagcg
     2281 tgccggacgc gccgctggtg ttcatcggct atggcctgca catgcccgag gccggccatg
     2341 acgatttcgc cggcgttgat ctgcgcggca agatcgcggt ggtgctgagc ggcgggccct
     2401 cggccgtcgg cgcgcccctg cgggcccacg cggcgcggga gcggtgggag gcgctgcaga
     2461 aggccggcgc ggttggtctg gtcagcatcc agaaccccaa gcaggtcgaa atcccatggg
     2521 agcgggcgag gctgcgcgcg gtcgagccgg ccatgcgcct ggccgatccg gccctcaacc
     2581 cggtgaaggg tgagctgttc accgcggtca tgaacccggc ctatgccaac aagctgtttg
     2641 cgccgtcggg ccgcacgctg gaagagctgc tggtcctggc cgaggccggc aaggcgctgc
     2701 cgggctttgc cctgaaccag agcctgcgcg ccaccgtggc gaccgagacc acccaggccg
     2761 tgtcatccaa cgttgcggcc ctgctgccgg gctctgatcc cgtgctgtcg aaggactatg
     2821 tggtgctgtc ggcccacctc gacggtctgg gcgtgggcca gcccattgac ggcgacgcga
     2881 tcaacaatgg cgcgatggac aatgccagcg gcgtggcctc gctgctggaa caggccgccg
     2941 ccctcaagac cggcgcgccg cccaagcggt cgatcctgtt cgtggctgtc accgccgagg
     3001 agcagggcct gctgggctcg caatatttcg ccgagcggcc cacggttccg aaggccgccc
     3061 tggtcgcca
//
LOCUS       DAOMEO010000290         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3555_length_3068_cov_2.730169, whole genome shotgun sequence.
ACCESSION   DAOMEO010000290 DAOMEO010000000
VERSION     DAOMEO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_3068_cov_2.730169"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..903
                     /locus_tag="PLH31_07670"
     CDS             229..903
                     /locus_tag="PLH31_07670"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQR89130.1"
                     /translation="MVKDRMAVLTAMMDQGVIPVFYHPDAEVCKKVIQACADGGAPCI
                     EFTNRGDFASHVFYEVTKHFATADERVIMGVGSIVDAPTAGIYIANGAKFVVGPILNA
                     DVAKVCNRRKIPYSPGCGSASEISYAEELGCEIVKVFPGSSVGGPDFVKAVLGPMPWT
                     RIMPTGGVDPDEASVKKWFGAGIVAAGMGSKLITPEMLDAGDYAGITKKVRETVDLIK
                     KVRGKA"
     gene            904..2010
                     /locus_tag="PLH31_07675"
     CDS             904..2010
                     /locus_tag="PLH31_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQR89131.1"
                     /translation="MTDNILNIRPASETKWDCASFGEVMLRFDPGFGRVRNARQFNVW
                     EGGGEYNVARAFKKCWGKRSTAVTALPVNDLGWLVEDLMMQGGVDTSHIIWRDFDGLG
                     RNTRVGLNFTEKGFGVRPALGCSDRGHSAASQIRPGEVNWEKLFGEEGVRWFHTGGIF
                     AALASNTAEAVIEAVEVARKYGTVISYDLNYRASLWKSQGGKEGAQKVNRHIAQYVDV
                     MIGNEEDFTACLGFEVEGLDEHISSIDPANFKKMIQTAVKEFPNFKVAATTLRNAKTA
                     SVNDWSAILYAGGEFYASMMRENLEIYDRVGGGDGFASGLAFGFMEGKGPQAAVEYGA
                     AHGALAMTTPGDTSMVRQAEVEAVMKGKGARVIR"
     gene            2283..>3068
                     /locus_tag="PLH31_07680"
     CDS             2283..>3068
                     /locus_tag="PLH31_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl CoA synthase"
                     /protein_id="HQR89132.1"
                     /translation="MVGIAAWGAYAPRLRLSRKAVVEANAWVAPNLKSKGKGERSMAN
                     WDEDALTMAVEAARDALGAGDDRSHVDALYFASTTAPFADRLNAGIVSAALTLEKSIT
                     ASDVTGSQRCGLTALADALAAVSGGAAKTALVAAGERRKARAVSALELDCGDGAAAFV
                     VTAEGGAADFLGRGSVTDDFVDHFRGTDGGFDYSWEERWIRDEGIVKLVPPAIRRALE
                     AAGVAPAEVAHFCFPSTFAGMAATLAKGLGIPAEAVRDNLAAVM"
BASE COUNT          543 a         1008 c         1019 g          498 t
ORIGIN      
        1 tggctcgacg ccctcacttt tgcctcggcg ggtgaggagc aggaatgttg gggaaggaag
       61 caaagcggcg cgaacgcgcc tttgtttgac gcagcggcag ccacgcagtg gcgtccgcgc
      121 gcgacgcggt ctaaacgatc gccctgagcc aataccggcg aaatcttccc tgcgacgggt
      181 ccttgagggg gcctgcacgc aagaacgatg cttttggagg actatgtgat ggtgaaagat
      241 cgtatggcgg ttctgaccgc catgatggac cagggtgtga tcccggtttt ctatcacccc
      301 gacgctgagg tctgcaaaaa ggtgatccag gcctgcgccg acggcggcgc gccctgcatc
      361 gagttcacca accgcggcga cttcgccagc cacgtcttct acgaagtgac caagcacttc
      421 gccacggccg acgagcgcgt gatcatgggc gtgggctcga tcgtcgatgc gccgaccgcc
      481 ggcatctaca tcgccaatgg cgccaagttc gtggttggcc cgatcctcaa cgccgacgtg
      541 gccaaggtct gcaaccgccg caagatcccg tattcgccag gctgcggcag cgcctcggaa
      601 atctcgtacg ccgaagaact gggctgcgag atcgtcaagg tattccctgg ctcgtcggtc
      661 ggcggcccgg acttcgtcaa ggcggtgctg ggccctatgc cctggacccg catcatgccg
      721 accggcggcg tcgatccgga tgaagcctcg gtgaagaagt ggttcggcgc gggcatcgtc
      781 gccgccggca tgggctccaa gctgatcacc ccggaaatgc tcgacgccgg cgactatgcc
      841 ggcatcacca agaaggtccg cgagacggtg gacctgatca agaaggttcg cgggaaggcc
      901 taaatgaccg acaacatcct caacatccgc ccggcttcgg aaacgaagtg ggattgcgca
      961 tccttcggtg aagtgatgct gcgcttcgac cccggctttg gccgggttcg caacgcccgc
     1021 cagttcaacg tctgggaagg cggcggcgaa tacaatgtcg cccgcgcttt caagaagtgc
     1081 tggggcaagc gctcgaccgc cgtcaccgcc ctgccggtga acgacctggg ctggctggtt
     1141 gaggacctga tgatgcaggg cggtgtcgac acctcccaca tcatctggcg cgatttcgac
     1201 ggtctgggcc gcaacacccg cgtcggcctg aacttcaccg aaaagggctt tggcgttcgt
     1261 ccggccctgg gctgcagcga ccggggtcac tcggccgcct cgcagatccg tcccggcgaa
     1321 gtgaactggg aaaagctgtt cggcgaagaa ggcgtgcgct ggttccacac cggcggcatc
     1381 ttcgcggcgc tggccagcaa caccgccgaa gccgtgatcg aagcggtgga agtggcccgc
     1441 aagtacggca ccgtgatctc ctacgacctg aactaccgcg catccctgtg gaagtcgcag
     1501 ggcggcaagg aaggggccca gaaggtcaac cgccacatcg cccagtatgt ggacgtgatg
     1561 atcggcaacg aagaagactt caccgcctgc ctcggctttg aagtcgaagg cctggacgag
     1621 cacatcagct cgatcgaccc ggccaacttc aagaagatga tccagacggc ggtcaaggag
     1681 ttcccgaact tcaaggtcgc cgccaccacc ctgcgcaacg ccaagaccgc ctcggtcaat
     1741 gactggtcgg cgatcctgta cgccggcggc gagttctatg cgtcgatgat gcgcgagaac
     1801 ctcgagatct atgaccgcgt cggcggcggc gacggcttcg cttcgggcct ggccttcggc
     1861 ttcatggaag gcaagggccc ccaggccgcc gtcgagtacg gcgcggctca cggcgccctg
     1921 gccatgacca ccccgggcga cacctcgatg gtgcgccagg ccgaggtcga ggccgtgatg
     1981 aagggcaagg gcgcgcgggt catccgctag tctgtctgaa ctggcatgcc aaagcccccg
     2041 gtcgcaggat cgggggcttt ttcatgggcg cagccacgcc ggctgatggc gagatgggcc
     2101 gcaaacctgc cgtcacggca cacttgccga ccccttccgc gagcgtaatc taaccgccga
     2161 taagtcgcga tcgaacgcga tgggagcgtg acagccgaaa ggggaagccg gtttcggcgc
     2221 ctgtcacgct ccagtgcaac agtgagagat atccgctctg cggatatctc ggggagcgga
     2281 acatggtcgg catcgccgcc tggggcgcct atgcgccgcg tctacgtctg agccgcaagg
     2341 cggtggtcga ggccaacgcc tgggtcgcgc cgaatctcaa gtccaagggc aagggcgagc
     2401 gctccatggc caactgggac gaggacgccc tgaccatggc cgtcgaggcc gcgcgcgacg
     2461 ccctgggcgc cggcgatgac cgcagccacg tcgacgccct ctatttcgcc tccacgaccg
     2521 cgccgttcgc cgaccggctg aacgcgggca tcgtctcggc ggcgctgacc cttgagaagt
     2581 cgatcaccgc cagcgacgtg accggctcgc agcgctgcgg tctcaccgcc ctggccgacg
     2641 ccctggcggc agtatctggc ggcgcggcca agacggcgct ggtcgcggcc ggcgagcgtc
     2701 gcaaggcccg cgcggtgtct gccttggagc tcgactgcgg cgacggcgcg gcggccttcg
     2761 tggtgaccgc cgagggcggc gcggccgact ttctggggcg cggcagcgtc accgacgatt
     2821 tcgtcgacca tttccgcggc actgacggcg gcttcgacta ttcgtgggaa gagcgctgga
     2881 tccgcgacga gggcatcgtc aagctggtcc cgcccgccat caggcgcgcc cttgaagcgg
     2941 caggtgtcgc ccctgccgag gtcgcccact tctgcttccc ctcgaccttc gccggcatgg
     3001 ccgcgacgct ggccaagggc ctgggcatcc cagccgaagc cgttcgcgac aatctcgccg
     3061 ccgtgatg
//
LOCUS       DAOMEO010000291         3068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3557_length_3068_cov_2.631265, whole genome shotgun sequence.
ACCESSION   DAOMEO010000291 DAOMEO010000000
VERSION     DAOMEO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_3068_cov_2.631265"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLH31_07685"
     CDS             <1..1232
                     /locus_tag="PLH31_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89133.1"
                     /translation="NQDQPILWNTLGTVMCSLGDGQQALTFFDEALRLAPAFGKGYHN
                     RAYARLDLGDAQGALEDCEAAMAYAESAEDLAMMEFARSTILLSLGRVSEGWAAYESR
                     FSHDLSDAPRFLIDAPRWSADQPLTGKHLMICTEQGLGDEVMFANMLPDVIESLGPEG
                     TLSLAVERRLIPLFQRSFPNADVTAHRTVSLEGRVRRTAPFVADWSKVDLWAPIGSLL
                     PVLRPSAESFPKRSHYLVADPERVAYWREQLEAAPKGPKVGLLWKSLKLNGERNRQFS
                     PFHQWQPVLETPGVTFVNLQYGDCEEEIALAKAEFGIDIWQPPGIDLKQDLDDVAALC
                     CAMDLIIGFSNATINLAGACGAPIWLLTGAAVWTRLGTDTYPWYPQARCFATEDFGDW
                     NPVMTRVAQALNETFTA"
     gene            1245..1964
                     /locus_tag="PLH31_07690"
     CDS             1245..1964
                     /locus_tag="PLH31_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQR89134.1"
                     /translation="MTLAILQARMSSSRLPGKVLADLVGAPMIVRQIERLKRARRVGR
                     IVVATSHESSDDPLAHVLGQAGIAVHRGDLNDVLGRFGTALEQWGETGDVVRLTADCP
                     LADPDVIDATIELHQASGADYTSNVAEPRTFPKGLDVEVMTTRALRVALAEAVDPHDR
                     EHVTPFLYRHPDRFRLAAHSQPADEGDVRWTVDRPDDLEFVRAVYETLYPDNADFSSS
                     DVRAFMRTRPDLASLGGDRRI"
     gene            1961..>2399
                     /gene="pseH"
                     /locus_tag="PLH31_07695"
     CDS             1961..>2399
                     /gene="pseH"
                     /locus_tag="PLH31_07695"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR03585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HQR89135.1"
                     /translation="MIRASLRSLDEADRARLLAWRASPHVSAFMYSDHRISAEEHDRW
                     FNGIAGDSRRRFWVIEADGAPVGLTNLADIDTTHGRATIARYIADPAVRGRGVGGFAE
                     FKVLDHALLQMGLRKLWSEVLETNAVAWHLHLSHGFQREALFRG"
     assembly_gap    2400..2409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2639..>3068
                     /locus_tag="PLH31_07700"
     CDS             2639..>3068
                     /locus_tag="PLH31_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQR89136.1"
                     /translation="MSQRFEGTSDYVATEDLKVAVNAAVALERPLLIKGEPGTGKTVL
                     AYEVAKAMGAPLLTWHIKSTTKAHQGLYEYDAVTRLRDSQLGEERVKDVRNYIKKGKL
                     WEAFESPVRPVLLIDEIDKADIEFPNDLLQELDRMEFYVYE"
BASE COUNT          556 a          981 c          955 g          566 t
ORIGIN      
        1 aaaaccagga tcaacccatt ctgtggaaca ccctgggcac ggtgatgtgt agccttggcg
       61 atggccagca ggccctgact ttttttgatg aggcgctgcg tctcgcgcca gccttcggca
      121 agggctacca caatcgcgcc tatgcccgcc tcgacctcgg cgacgcccaa ggagcgcttg
      181 aagactgcga agcggccatg gcctacgccg aatccgccga ggatctggcg atgatggagt
      241 ttgcgcggtc cacgatcctg ttgagtctag gtcgcgtgag cgaaggctgg gcagcctatg
      301 agtcacgctt ttcgcacgac ctttcggatg ctccccggtt cctgatcgac gccccgcgct
      361 ggtcagcgga ccaacccctg accggcaagc acctgatgat ctgcaccgag caaggcctgg
      421 gcgacgaggt gatgttcgcc aacatgctgc ccgacgtcat tgagtccctg gggccggaag
      481 gaacgctttc attggcggtg gagcgccgcc tgatcccgtt gttccagcgc tcgttcccca
      541 atgccgatgt gaccgcccac cgcacggttt cgcttgaggg ccgcgtgcgc cgcacggcgc
      601 cgttcgtcgc cgactggagc aaggtcgacc tgtgggcccc catcggctcg ctgctacctg
      661 tcctgcgacc ttccgcggag tccttcccga agcgctccca ctatctggtg gctgatcccg
      721 aacgcgtcgc atactggcgc gagcaactgg aggccgcgcc caagggccca aaagtcggtc
      781 tgctgtggaa aagcctgaag ctgaacggcg aacgtaaccg gcagttttca ccattccatc
      841 agtggcagcc ggtcctggaa accccgggcg tcaccttcgt gaatttacag tacggcgact
      901 gcgaagagga aatcgccctg gccaaggccg agttcggcat cgacatctgg cagccccccg
      961 gcatcgacct gaagcaggat ctcgacgacg tagccgccct gtgttgcgcc atggacctga
     1021 taatcggctt ctccaacgcc accatcaacc tggccggcgc ttgcggcgcg ccaatctggc
     1081 tgcttacggg cgcagcagtc tggacccgct tgggaaccga tacctatccc tggtatccgc
     1141 aggcgcgctg ctttgcgacc gaggactttg gcgactggaa tccggtcatg acccgggtcg
     1201 cgcaggcgtt gaatgagacc ttcacagcct aggaaagcaa ggtcatgacc ctggcgatcc
     1261 ttcaggcccg gatgtcctca agccggttgc ctggcaaggt gttggccgat ctggttggcg
     1321 cgcctatgat cgtgcgccag atcgaacgcc tgaagcgcgc ccgccgcgtg ggcaggatcg
     1381 tggtggctac ctcgcatgag tccagcgacg acccgctcgc gcacgttctc ggtcaggccg
     1441 ggatcgccgt gcatcggggc gatctcaatg acgttctggg tcgatttggc accgcgctgg
     1501 agcaatgggg cgagaccggc gatgtggtgc gcctgaccgc cgactgcccc ctcgccgatc
     1561 cggacgtcat cgacgccacg atcgagctgc accaggccag cggcgcggac tacacgtcca
     1621 acgtcgccga accccgcacc tttcccaagg gactggacgt cgaggtcatg accacccgag
     1681 ccttgcgcgt cgccctggcc gaagccgtgg atccccatga ccgcgaacat gtgacgccgt
     1741 ttctctatcg tcatcccgac cgcttccgct tggcggccca ttcccagcct gccgacgagg
     1801 gggacgtgcg gtggactgtc gaccgcccag acgacctcga gtttgtgaga gcggtctacg
     1861 aaaccctgta cccggacaat gcggactttt cctcctcgga tgtccgcgcc ttcatgcgga
     1921 cccgccctga tctggcctcg ctggggggag atcgtcggat atgatcaggg cctctctgcg
     1981 atcgctggac gaagcggacc gcgcccggct gctagcctgg cgcgccagcc cccacgtctc
     2041 ggcgttcatg tattcggatc atcggatcag cgccgaggag catgatcgct ggttcaacgg
     2101 gatcgcaggc gacagccgcc gccgtttctg ggttatcgag gccgatggcg ctccagtcgg
     2161 cctgactaat ctggctgaca tcgacacaac ccatggccgc gcaacgattg cgcgctatat
     2221 cgctgatccg gctgtacgcg gccgcggcgt gggcggattt gccgagttca aggtccttga
     2281 tcacgccctt ctgcagatgg gactgcgtaa actctggtcc gaggtgctgg agacgaacgc
     2341 cgtggcttgg cacctgcacc tgtcgcacgg ctttcaacgc gaggccctgt ttcgcgggcn
     2401 nnnnnnnnna acggccagcc tgccaatgtg gtcggactcg gtctgctgag cgaagaatgg
     2461 cgcatgatcc gaccggtcgc ccgccagcgc ctgatcgaca agggttacgt agcggaggcc
     2521 ttggacggcc caatcccaga ctgaatttcg gtcaagccat acccttgcta aaggcgcggc
     2581 ggtcagtagt ttgacgaact tcgaacgctt gttcgaacga tcttgaccgg ggataggcat
     2641 gagccagcgc ttcgaaggca cttccgacta tgtggcgacc gaggatctga aggtcgcggt
     2701 aaacgccgct gtggcgcttg aacgtccctt gctgatcaag ggcgaacccg gcacgggcaa
     2761 gacggtgctg gcctacgagg tcgccaaggc catgggcgcg ccgctgctga cgtggcacat
     2821 caagtcgacg accaaggcgc accagggcct atacgaatac gacgccgtca cccgtctgcg
     2881 cgatagccag ctgggcgaag agcgggtcaa ggacgtcagg aactacatca agaagggcaa
     2941 gctgtgggag gctttcgaaa gcccggtgcg ccctgttctg ctgatcgacg agatcgacaa
     3001 ggccgatatc gagttcccga acgatctgct gcaagagctc gatcggatgg agttctatgt
     3061 ctacgaaa
//
LOCUS       DAOMEO010000292         3058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3567_length_3058_cov_2.668332, whole genome shotgun sequence.
ACCESSION   DAOMEO010000292 DAOMEO010000000
VERSION     DAOMEO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3567_length_3058_cov_2.668332"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1161
                     /locus_tag="PLH31_07705"
     CDS             181..1161
                     /locus_tag="PLH31_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQR89137.1"
                     /translation="MNIFQQSAAARYGLAVFATVAGLVARLAVENWIPPGFPYVTFFP
                     AVVVTAYFAGLRPAILCGVLSGLAAWWFFIPPVHSFQLTAPTIVALVFYAFVIAVDIF
                     FIDGMRQAMRQVSAERERYARLAESRDLLYRELHHRVSNNLQVVGALLRLQAAGVTDG
                     DARRALSEAGGRIEVIARIQRELHNQAGDPAPFRDFARALLDDASAAAGARITLTLEG
                     GDKPLHPDQATAVTLVMLESFNNALEHAFENGQGAVRVALDQTGNQHVLTVADDGKGP
                     PEGFDPGQSKSLGLRIVRAMASQLCGRFEMSRLDGWTICRLTYPPNVDPL"
     gene            complement(1156..>3058)
                     /locus_tag="PLH31_07710"
     CDS             complement(1156..>3058)
                     /locus_tag="PLH31_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89138.1"
                     /translation="IENIEKRVAAGERPYAAAMVGADAIGLAVVATTFTIVAVFAPVA
                     FMPGIPGQFFREFGLTVAVAVLFSLVVARLLTPLLAAYFLKPATNPHPRKPFKGMYRN
                     VLEWALDHRIVACIIGGVIFAFSIFMASGLPAGFQPPANNNYYYLKVQGPPGATAADM
                     DRAVQSITRLFKARPETAHVFAQIGTVIAASGPGGGSSGSDLRDGTVTIVLHDERELR
                     VPEIKALMRDKLMDLPDVRVNLLSDDGSAEVSTILTSEDGPALERAALTLERQMRGLK
                     TVADPRPASPPSGPEIVIRPRADEAARLGVSAADIAAVARVATVGDIDANVAKLTDGE
                     RRLPIRVRLADGVRSDLAQIKALQLPTASGGYTRLDAVADVEFQAGPAKIERYGRKRQ
                     LTTEADLQPGVVLGSATAEVDNLPIMKNLPAGVEKATGGTEEAMGELFGGFAIALLSA
                     IFLVFGVMALLFGSFFKPITIMSALPLAIGGAFAGLLLCGMSMSIPSLIGLLMLMGLA
                     AKNSILLVDYAIEEERAGKSQRDAILEACRERARPIIMTTLAMMAGMLPTALGIGKGS
                     EFRQPMAVAVIGGLITSTVLSLVLVPVVYEIVDDFENWLKPKLARWITPREAPADAAT
                     ANGAPTDRL"
BASE COUNT          486 a         1010 c         1057 g          505 t
ORIGIN      
        1 cagtcacctg ttgatctaag cgaaggcccc ggcggaaacg ccggggcctt ttgctttggg
       61 cgaggattgg agccaaaggg ggggctcagc ttgccgtggg tcgggtagcg gcttgacgct
      121 ggcggcgctt gacgtcagca ggggatagcc gcccattttt tgccgggcgt gggagacgtc
      181 gtgaacatat tccagcaatc ggccgcagcg cgttatggtc tggcggtatt tgccaccgtc
      241 gccggcctgg tggcgcgact tgccgtggaa aactggatcc cgcccggctt tccctatgtt
      301 accttctttc ccgccgtggt ggtcacggcc tatttcgccg gcctgcgccc cgcgatcctg
      361 tgcggtgtgc tgtcgggcct ggccgcctgg tggttcttca ttccgccggt ccatagcttc
      421 cagctgaccg cgccgaccat cgtggctctg gttttctatg ccttcgtgat cgcggtcgac
      481 atcttcttca tcgacgggat gcgccaggct atgcggcagg tgtccgccga acgcgagcgc
      541 tatgcccgcc tggccgagag ccgcgacctg ctctatcgcg aactgcacca ccgggtgagc
      601 aacaacctgc aggtcgtggg cgccctgctt cgcctgcagg cggcgggcgt caccgatggc
      661 gatgcgcgcc gcgccctgtc cgaggccggc ggtcgcatcg aggtgatcgc ccgcatccag
      721 cgcgaactgc acaaccaggc cggggatccc gccccgtttc gcgattttgc cagggccctg
      781 ctggacgatg cgagcgccgc agccggggcc cgcatcaccc tgaccctgga gggcggcgac
      841 aaacccctgc accctgacca ggccacggcc gtgaccctgg tgatgctgga gagcttcaac
      901 aacgcgctgg aacacgcctt cgaaaacggc cagggcgcgg tgcgtgtcgc cctcgatcag
      961 accgggaacc agcacgtgct gaccgtggcc gacgacggca agggcccgcc cgagggcttc
     1021 gacccaggcc agtccaaaag cctgggcctg cgcatcgtcc gcgccatggc cagccagctg
     1081 tgcggccggt tcgagatgag ccgtctcgac ggctggacga tctgtcgcct gacctacccg
     1141 ccgaacgtgg atcccttata gccggtcggt cggagcgccg ttggctgtcg cagcatcggc
     1201 cggggcctcg cgcggcgtga tccagcgggc gagcttgggt ttcagccagt tttcgaaatc
     1261 atcgacgatc tcatagacca cgggcaccag caccagcgac aggaccgtcg aggtgatcag
     1321 gccgccgatc accgctacag ccatcggctg gcggaactcc gatcccttgc cgatccccag
     1381 ggccgtgggc agcatgccgg ccatcatcgc cagggtggtc atgatgatgg ggcgggcccg
     1441 ctcgcgacag gcttcgagga tggcgtcgcg ctggctcttg ccggcccgct cctcctcgat
     1501 ggcgtagtcg accagcagga tcgagttctt ggctgcaagg cccatcagca tcaaaaggcc
     1561 gatcagcgac gggatcgaca tgctcatccc gcacagcagc aggcccgcaa aggccccgcc
     1621 gatggccaga ggcagggccg acatgatggt gatcggcttg aagaagctgc caaacagcag
     1681 ggccatcacg ccaaacacca ggaagatggc cgacagcagg gcgatggcga agcccccgaa
     1741 cagctcgccc atcgcttctt cagtaccgcc cgtggccttt tcgacgccgg cgggaaggtt
     1801 tttcatgatc ggcagattgt cgacctctgc ggtggccgaa cccaggacga cgccgggctg
     1861 cagatcggcc tcggtggtca gctggcgctt gcggccatag cgttcgatct tggccggacc
     1921 ggcctggaac tcgacatcgg ccacggcgtc cagtcgggta tagccgccgg acgcggtggg
     1981 cagttgcagg gccttgatct gggccaggtc gctgcggacg ccgtcggcca gccgtacccg
     2041 gatgggcagg cgccgctcgc cgtcggtgag cttggcgaca ttggcgtcga tgtcgcccac
     2101 tgtggccacg cgggcgacgg cggcgatatc ggcggccgag acgcccaggc gggccgcctc
     2161 gtcggcgcgc gggcggataa cgatttccgg gccgctgggc ggcgaggcgg gccttggatc
     2221 ggcaacggtc ttcaggccgc gcatctggcg ctccagcgtc agggcggcgc gttccagagc
     2281 ggggccgtcc tcgctggtca ggatggtgga gacctcggcc gagccgtcgt cgctgagcag
     2341 attgacccgc acatccggca gatccatcag cttgtcgcgc atcagggcct tgatctcggg
     2401 gacccgcagc tcgcgctcgt cgtgcagcac gatggtcacc gtgccgtcgc gcaggtcgct
     2461 gccgctggag ccgccgccgg gaccgctggc ggcgatcacc gttccgatct gggcgaagac
     2521 gtgcgcggtt tcgggccggg ccttgaacag cctggtgatc gactggaccg cgcggtccat
     2581 gtcggccgcc gtggcgcccg gcgggccctg aaccttgagg taatagtagt tgttgttggc
     2641 cggcggctgg aagccggcgg gcaggccgct ggccatgaag atcgagaagg cgaagatcac
     2701 cccgccgatg atgcaggcca cgatccggtg gtcgagcgcc cactccagca cgttgcgata
     2761 catccccttg aacggcttgc gcggatgggg attcgtggcg ggcttgagga aataggcggc
     2821 cagcagcggc gttagcagac gcgccaccac cagcgagaac agcaccgcca ccgcgacggt
     2881 caggccaaac tcgcggaaga actggccggg gatgccgggc atgaaggcca caggcgcgaa
     2941 caccgccacg atggtgaagg tggtggccac caccgccaga ccgatggcgt cggcgccgac
     3001 catggcggcg gcatagggcc gttcgccggc ggcgacgcgt ttctcgatat tctcgatc
//
LOCUS       DAOMEO010000293         3055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3570_length_3055_cov_2.942333, whole genome shotgun sequence.
ACCESSION   DAOMEO010000293 DAOMEO010000000
VERSION     DAOMEO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_3055_cov_2.942333"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="PLH31_07715"
     CDS             <1..1986
                     /locus_tag="PLH31_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89139.1"
                     /translation="NNDLILAIRGVAPGGVLPNVDQAVGTYVDGVYYARPEGSNLAMV
                     DIAAAEVLRGPQGTLFGRNTIGGALNITTNKPTYEFGGSLKLGYGNYNAIAGTGMVNL
                     PIVADKLAARLVYSHVEHDGYGYNPSLKSDAADQNDDFIRASVRADLSPDLRVDVGFD
                     HYAGSNHQPLWVLNSYQAGLTAAQYAPYVAGPESRISYAGNNPVNKSKVFDWTGTITA
                     DLGFATLKTISGYRNIDFEGAADLDGTPFPTADVRTFELDGDQVSQEIQLYGTTFADR
                     LNWTAGAYYFREKIRNSPITRVPTAIQDNTIRPTNESVSLFGQASFEILPRLRLTAGV
                     RAVKDTRSMVYTPARFAVAAASSTNPQPPASAVTPAACPFTAIGLNQAPGGCLYTPHD
                     IEFNTVPFTIGLDYRLPGDGLLFGKFSKGYRSGGFQQASGTTAAFFRPFDEESVGSYE
                     AGAKLSFFDKRLRLGLSGYFSAFTGIQQNAILSASPVIIAVLNSGKAEIYGGEFEATA
                     LLGGLRLNASLGLIHPEFTDGPYKGSEVPTVAKTTFALSADYPIELSAGRLDLHVDYN
                     YRSEVFFLNTVTITGAGPVPLTPFQRRSISQDGYGLLNAQVAFTLSNAPVTVAVYGKN
                     LADEYFGARSGSFAAANFNTVVIGAPRTYGVNVSYAF"
     gene            2091..2981
                     /locus_tag="PLH31_07720"
     CDS             2091..2981
                     /locus_tag="PLH31_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQR89140.1"
                     /translation="MRLLSFELNGAPVMGVRLDEEVVDLSVAAPDLPRDIRALLAAGP
                     EALAAAERAAEAAPGHARHPLQGLRHLVPIPRPDKIIGLGKTYMKHVAEMKAEASSFP
                     GMFLRVPDSLVAHNMPIWRPEASDTLDYEGELMVVIGRGGRMISRENALEHVAGYACH
                     NDGSVRAYNYIPSAVTAGKNFLNTGGFGPEIVTADELPPGCKGLRLTTHVNGDLRQSA
                     DIAELHWDVAHLVHLMSTIFTLYPGDLIATGTPSGCAAGSANPQWLVPGDVVRVEIEG
                     VGVLVNPIADAPKAAPDDRS"
BASE COUNT          530 a         1032 c          958 g          535 t
ORIGIN      
        1 aacaatgacc tgatcttggc gatccgcggc gtggcgccgg gaggcgtcct tcccaatgtc
       61 gatcaggcgg tcggcaccta tgtggacggc gtctactacg cccgtcccga gggcagcaat
      121 ctggccatgg tggatatcgc cgcggccgag gtcctgcgcg gtccgcaagg cacgctgttt
      181 ggccgcaata ccatcggcgg ggcgttgaac atcaccacca acaagccgac ctatgagttc
      241 ggcggatcgc tgaagctggg ctacggcaac tacaacgcca tcgccggaac cggcatggtc
      301 aatctgccga tcgtcgccga caagttggcg gcgcgactgg tctacagcca cgtcgaacac
      361 gacggctacg gctacaatcc gtcgctgaag agcgacgcgg ccgatcagaa cgacgatttc
      421 atccgcgcca gcgtccgcgc cgacctgtcg cccgatctgc gggtggatgt cggtttcgac
      481 cactatgccg gctccaacca ccagccactg tgggtgctca actcgtacca ggccgggctg
      541 acggccgcgc aatacgcgcc ctatgtcgcg ggtccggagt cgcgcatcag ctacgcgggc
      601 aataatcccg tcaataagtc caaggtcttc gactggaccg ggacgatcac ggccgacctc
      661 ggatttgcga ccctgaagac gatatcgggc tatcgcaaca tagatttcga gggcgccgcg
      721 gacctggacg ggacgccgtt tcccacggcg gacgtgcgca cgttcgagct cgacggagac
      781 caagtctcgc aggaaatcca gctctacggg accaccttcg ccgatcggct gaactggacg
      841 gcgggcgcct actatttccg tgagaagatc cgcaactcgc cgatcacccg ggtgccaacc
      901 gcgatccagg acaacaccat ccggccgacg aatgaatcgg tcagcctgtt cggccaggcc
      961 agcttcgaga tcttgcctcg cctgcgcctc acggccgggg tgcgcgcggt caaggatact
     1021 cgcagcatgg tctatacccc ggcccgcttt gccgtggcgg cggccagttc gaccaatccc
     1081 cagcccccgg cctcggcggt gacgccggcg gcgtgcccct tcacggccat cggcctcaac
     1141 caggcgccgg gcgggtgcct ctacacgccc cacgacatcg aattcaacac cgtcccgttc
     1201 acgatcggcc tggactatcg gttgccgggg gacgggctgt tgtttggcaa attctccaag
     1261 ggctaccgat ctggtggatt tcagcaggcc agcggtacga ccgccgcctt cttcaggccc
     1321 ttcgacgagg agagcgtggg aagctatgag gccggcgcca agctctcgtt cttcgacaag
     1381 cgcttgaggt tgggtctgag tggctatttc tcggccttca cgggcatcca gcagaacgcc
     1441 atcctttcgg ccagtcccgt catcatcgct gtcctcaact cgggcaaggc ggagatctac
     1501 ggcggtgagt tcgaggcgac cgccctgctg ggcggcttgc gcctcaacgc ctcgctcggt
     1561 ctgatccatc ccgagttcac cgacggtccc tacaaggggt cggaagtgcc gacggtcgcc
     1621 aagaccacct tcgcgctcag cgccgactat ccgatcgaac tgtcggcggg gcgactggat
     1681 ctgcatgtcg actacaacta tcgatccgag gtgttcttcc tgaacacggt gacgatcacc
     1741 ggggcggggc ccgtccccct gacgccattt cagcgccgct cgatcagcca ggacggctat
     1801 ggactgttga acgcccaggt cgcgttcacc ctctcgaatg ctccggtaac cgtggccgtc
     1861 tacggcaaaa acctcgccga cgaatacttc ggcgcgcggt cgggatcctt cgccgcggcc
     1921 aacttcaata cggtggtgat cggcgcgccg cgcacctatg gcgtcaacgt ctcctacgcc
     1981 ttctgatcga cagccccggc ccgcgttccg gggcgcaacg cggcgcgatc ctcgcggatc
     2041 gcgccgtctc ttttcccggc tcgcaggccg gtcgccgaga ggagagtttg atgcgactac
     2101 tatcattcga gttgaacggc gcccccgtca tgggcgtgcg cctggacgaa gaggtcgtcg
     2161 atctgagcgt cgccgcgccg gatttgccac gggacattcg tgcgttgttg gcggccggtc
     2221 cagaggcctt ggcggcggcc gagcgggccg cggaggccgc gccaggccac gcgcgtcatc
     2281 cgctccaggg cctgcgtcac ctcgttccaa ttccccggcc cgacaagatc atcggcctgg
     2341 gcaagaccta catgaagcac gtcgccgaaa tgaaggccga ggcgtcgtcg tttccaggaa
     2401 tgttcttgcg ggtgccggat tccctggtgg cccacaacat gccgatctgg cggccggagg
     2461 cctcggacac gctcgactac gaaggcgagt tgatggtggt catcggccgg ggagggcgca
     2521 tgatatcgcg cgaaaacgcc cttgagcacg tcgccggtta cgcctgtcac aacgacggca
     2581 gcgttcgcgc ctacaactac attccctcgg cggtcacggc cggcaagaac ttcctcaata
     2641 cgggcgggtt cggccccgag atcgttaccg ctgacgagct accgcccggc tgcaaggggc
     2701 tcaggctcac gacccacgtc aatggtgact tgcggcagag cgcggatatc gccgagctgc
     2761 actgggacgt cgctcatctg gtccatctga tgtcgaccat cttcaccctc tatcccggcg
     2821 acctgatcgc caccggaacg ccgtcgggct gcgcggcggg gtcggccaat ccgcaatggc
     2881 tggtcccggg cgacgtggtg cgcgttgaga tcgagggcgt cggcgtgctg gtcaacccga
     2941 tcgcggacgc gcccaaggcg gcgccggacg atcggtccta gcgcaccatc atcaggccgc
     3001 cgtccaccgc cagggtctgg cccgtgatga accgcgccgc gtcgctggcg agaaa
//
LOCUS       DAOMEO010000294         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3575_length_3051_cov_2.605140, whole genome shotgun sequence.
ACCESSION   DAOMEO010000294 DAOMEO010000000
VERSION     DAOMEO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_3051_cov_2.605140"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..572
                     /locus_tag="PLH31_07725"
     CDS             384..572
                     /locus_tag="PLH31_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89141.1"
                     /translation="MPKGFEDQAGGPLEAAIKRTRWLVRRDLTGAEIGGPDLPAVIAD
                     FAADARPLLDFGWKALRA"
     gene            669..1067
                     /locus_tag="PLH31_07730"
     CDS             669..1067
                     /locus_tag="PLH31_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89142.1"
                     /translation="MSSTVTSSSRRRGLDHFEPEDVDPQAQRRLRGTLEQIDYTAFAS
                     NKALIGQALGHVDATHFQHMAVAAAQARAQWVAAAVAMTETAHTPAPDQIARLAQMRM
                     AYEELAEAYEAMRRMVERGYVMFRPVPNPG"
     gene            1143..1481
                     /locus_tag="PLH31_07735"
     CDS             1143..1481
                     /locus_tag="PLH31_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQR89143.1"
                     /translation="MNGRKALVRDYRDRKVEAGIYAVRCATSGEAWVGATPDLSIRQN
                     GLWFGLRQGSHPERSLQAAWTAHGEAAFSYEPLETIDDETLGPIGKSSLLKERRAAWI
                     ESLGAKGLNR"
     gene            1478..2065
                     /locus_tag="PLH31_07740"
     CDS             1478..2065
                     /locus_tag="PLH31_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HQR89144.1"
                     /translation="MSYAVFKGAERVATGSSLDAALAAQRLADDPSADLVLIFDPEGR
                     QVDFDLSGDPADLEARLAPAEDAAAPRGRGRPRLGVVSREVTLLPRHWDWLAAQPGGA
                     SVALRKLVEAARRQGEGPERIRRSREAAYRFATAMAGDRAGFEAAMRALFAGDDAAFE
                     ARLVDWPADIAAQLRTYAAEAFGADEASVQVEARG"
     gene            complement(2044..2439)
                     /locus_tag="PLH31_07745"
     CDS             complement(2044..2439)
                     /locus_tag="PLH31_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR89145.1"
                     /translation="MARHLALIALLVGDYGEALSFYVGKLGFDLIEDTDMGAGKRWVV
                     VSPGDGGSRFLLAKASDEHQAARIGDQGGGRVWLFLHTDDFAGDHARMSAAGVRFLET
                     PRHEPYGSVAVFEDLYGNRWDLLQPRAST"
     gene            2560..3048
                     /locus_tag="PLH31_07750"
     CDS             2560..3048
                     /locus_tag="PLH31_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89146.1"
                     /translation="MSIRLALALSLAMAGSSAIAGAPETRITPVAAADLPPAVLAVVT
                     KAAPGLKVIEAELKEREGRRYFDVEGVLPSGAEIEFDLLEKNGVWEIVETQRDIAWAD
                     APAVVRTAAGKAQPVRVIESTQNDGLVIYELFAPGQPRTPALEVSFKDGVAKVLAETW
                     PH"
BASE COUNT          505 a         1048 c         1036 g          462 t
ORIGIN      
        1 gaaacaggta tccaaccagc acgccggccg cgacggccat gagcacctgc acatacagga
       61 tcttgtagaa aggctgccgc atgtcgtccc ccccgcgcgg ggctggcccc gcccacggtt
      121 tcctaatcac caagcgcgac aaatgcacgc ctgcgctgcc atgggcgcgc gcagcaaaag
      181 tcggcggcgg cgtgtcggca tcgatgctag tcctgcgtcc tcgggagaca gggagacccc
      241 catgcaatac gccattcttt gctaccattc cgaggatgtg gtctgcgcct ggagccggga
      301 gcatgacgag gccgtcatgc agcgcctgaa caacggtcgc ggccagcggg ctgccgctgg
      361 agcccggcga gccgaccaag cggatgccca agggcttcga ggatcaggcc ggcggcccgc
      421 tggaagcggc gatcaagcgc acccgctggc tggtgcgccg cgacctgacc ggcgccgaga
      481 tcggcgggcc ggacctgccc gcggtcatcg ccgacttcgc ggcagacgcc cggccgctgc
      541 tggatttcgg ctggaaggcg ctgcgcgcct gacgcgtcgc cgacggcaat gaccttttaa
      601 gcagtccggc gcacgctctc tccagacccg gcggccatca cgaccccata gccgggcggg
      661 aggacgccat gtcgtcgacc gtcacctcca gttcccgccg tcgcggcctg gatcatttcg
      721 aacccgagga tgtcgatccc caggcccagc gccggctgcg cggcacgctg gagcagatcg
      781 actataccgc ctttgcctcc aacaaggcgc tgatcggtca ggccctgggc catgtcgacg
      841 ccacccattt ccagcatatg gcggtcgccg ccgcccaggc tcgcgcccag tgggtggccg
      901 ccgccgtcgc catgaccgaa accgcccaca cccctgcgcc ggaccagatc gccaggctgg
      961 cccagatgcg catggcctat gaggagctgg ccgaagccta tgaggccatg cggcggatgg
     1021 tcgagcgggg ctatgtgatg ttccgccccg tgccaaaccc tggctaggcc gccaagttat
     1081 atacccgggt aatattgact ctcctattta tccgggtaaa aagcgcgcca caggagggtc
     1141 ccatgaacgg acgcaaggcg ctggtgcgcg actatcgtga ccgcaaggtc gaggccggga
     1201 tctatgcggt gcgctgcgcc accagcggcg aggcctgggt cggcgcgaca ccggacctgt
     1261 cgatacgcca gaacggcctc tggttcgggc tgagacaggg atcacatccc gagaggagtc
     1321 tgcaggccgc ctggaccgcc catggcgagg ccgccttcag ctatgagcct ctggagacca
     1381 tagacgacga gaccctgggc ccgatcggca aatcctcgtt gctgaaggaa cgccgcgccg
     1441 cctggatcga gagcctgggc gccaaggggc tgaaccgatg agctacgcgg ttttcaaggg
     1501 cgccgagcgc gtggcgacgg gatccagcct tgatgccgcc ctggccgccc agcgtctggc
     1561 ggacgacccg tctgccgact tggtgctgat ctttgatcct gagggccgcc aggtggattt
     1621 cgacctcagc ggcgaccccg ccgaccttga agcgcggctg gccccggcgg aggatgccgc
     1681 tgcgccgcgc ggtcgcggcc gccccaggct gggcgtcgtg tctcgggagg tcaccctgct
     1741 gcctcgccac tgggactggt tggccgctca gcccgggggg gcttcggtcg cccttcgcaa
     1801 gctggtcgag gcggcgcgac ggcagggcga agggccagag cgcatccgcc gctcgcgcga
     1861 ggcagcctat cgcttcgcca cggccatggc cggagatcgc gctggcttcg aggcggcgat
     1921 gcgggcgctg ttcgcgggcg acgacgccgc attcgaggcg cgtcttgtcg actggcccgc
     1981 cgacatcgcc gcccagctgc gcacctatgc cgccgaggcg ttcggcgcgg acgaggcctc
     2041 ggttcaggtc gaggcgcggg gctgaagcag gtcccagcgg ttgccgtaga ggtcttcgaa
     2101 caccgccacg ctgccatagg gttcgtggcg gggcgtttcc agaaagcgga ccccggcggc
     2161 gctcatgcgc gcgtgatcgc cggcgaagtc gtcggtgtgc aggaacagcc agacccggcc
     2221 accgccctgg tcgccgatcc gcgccgcttg atgctcgtcc gaggccttgg ctagcagaaa
     2281 gcgcgagccg ccatcgccgg gcgacaccac gacccagcgc ttgcccgcgc ccatgtcggt
     2341 atcctcgatc agatcgaagc ccagcttgcc gacatagaag ctcaacgcct cgccatagtc
     2401 gccgacgagc agggcgatga gggcgaggtg gcgggccatg gcgactcctt gaacaggcgt
     2461 gcttgatagc atcaccgatc tggccctcgc atcgttgatg aaaggcgcga cgccacatga
     2521 taccggttac cactcacgcc ctgttcgagg atatccgtca tgtcgatccg tcttgccctg
     2581 gctctttcgc tcgccatggc cggttcatcc gccatcgcgg gcgcgcccga aaccaggatc
     2641 acgccggtgg ccgccgccga cctgccgccc gctgtgctgg cggtcgtcac caaggccgcg
     2701 ccaggcctga aagtgatcga agccgagctg aaggagcggg aaggccgccg ctatttcgat
     2761 gttgaaggtg ttctgcccag cggcgccgag atcgagttcg acctgctgga aaagaacggc
     2821 gtctgggaga tcgtcgagac ccagcgcgac atcgcctggg ccgacgcccc ggcggtggtg
     2881 cggacggcgg ccggcaaggc ccagccggtg cgggtgatcg agagcaccca gaacgacggc
     2941 ctggtgatct atgagctgtt cgccccgggc cagcccagga ccccggccct ggaagtcagc
     3001 ttcaaggacg gcgtcgccaa ggtgctggcc gagacctggc cgcactaagg c
//
LOCUS       DAOMEO010000295         3038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3589_length_3038_cov_2.502514, whole genome shotgun sequence.
ACCESSION   DAOMEO010000295 DAOMEO010000000
VERSION     DAOMEO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_3038_cov_2.502514"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="serS"
                     /locus_tag="PLH31_07755"
     CDS             <1..839
                     /gene="serS"
                     /locus_tag="PLH31_07755"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQR89147.1"
                     /translation="GMDFEAAARMSGARFVVLKGQIARLERALGQFMLDMQTVKHDYT
                     EVSPPLLVKDEALFGTGQLPKFAEDLFRTTADHWLIPTAEVSLTNIVREQITEEAQLP
                     LRMTALTPSFRSEAGASGRDTRGMIRQHQFYKVELVSITTPDQSEAEHERMVGCAEAV
                     LKALNLPFRTMLLCNGDMGFGARKTYDLEVWLPSQNTYREISSCSNCGDFQARRMDAR
                     FRKAGEKGTQYVHTLNGSGLAVGRTLVAVLENYQDETGGITIPEALVPYMGGVTYIGG
                     EG"
     gene            976..1770
                     /gene="surE"
                     /locus_tag="PLH31_07760"
     CDS             976..1770
                     /gene="surE"
                     /locus_tag="PLH31_07760"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQR89148.1"
                     /translation="MRILLTNDDGIHAPGLVALEKIARALSDDVWICAPEYEQSGASR
                     ALTLSDPLRVRRLDDRRFAVEGTPTDCVMMAVQQLIEGPAPDLVLSGVNRGQNLAEDV
                     TLSGTVAGAIEGMALGIRSIALSQAMNYFHDEVVAHWETAQTYGPGIIQRLLEVGWPA
                     DVIMNINFPSLPPEEVTAVEVTRQGFRDGHLRHWDKRTDLRGRDYYWMGFTAKASEPA
                     PGTDLKAVYEGKISVTPLHIDLTHNETVHALKGVLGGAPPKLRVEA"
     gene            1767..2426
                     /locus_tag="PLH31_07765"
     CDS             1767..2426
                     /locus_tag="PLH31_07765"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQR89149.1"
                     /translation="MTGKAKAAAAADLPRLMQALRDQGITDPQVLKAIEKTPRDLFTP
                     DLFKERSWEDSALPIACGQTISQPYIVGLMTQALTIEPRSRVLEIGTGSGYQTTILSH
                     VSRLVYTIERYRTLMKEAEARFTKLGLTNVITKFGDGGEGWAEQAPFDRIMVTAAAED
                     DPKRLLSQLKPNGVLVAPIGKGPVQSLRRYAGDGKGGFKVEALCDVRFVPLLDGVARD
                     Q"
     assembly_gap    2557..2566
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2567..>3038
                     /locus_tag="PLH31_07770"
     CDS             <2567..>3038
                     /locus_tag="PLH31_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HQR89150.1"
                     /translation="TLAACETTSSPQYPAREGQPGGSPPRAQTPNFPITQAPPSEAVI
                     DTTPAPSEVIRREPIPVEAAPPPPTYSEPPVTRTELAPPPPPPPPAQLRPAAPTIITT
                     VGGPVVDVPGKPQMRIVESGDALDAIARGFGTTRAELVKLNDLEPPYRLKLGQK"
BASE COUNT          530 a         1044 c          990 g          464 t
ORIGIN      
        1 gcggcatgga cttcgaagcc gccgcccgca tgagcggcgc gcgcttcgtg gtgctgaagg
       61 gccagatcgc gcggctggag cgggcgctgg gccagttcat gctggatatg cagacggtca
      121 agcacgacta caccgaggtc agcccgccct tgctggtcaa ggacgaggcg ctgttcggga
      181 ccgggcaact gccgaagttc gccgaggatc tgttccgcac cacggcggac cactggctga
      241 tccccactgc cgaagtctcc ctgaccaaca tcgtccgcga gcaaatcacc gaagaggctc
      301 agctgccgct gcggatgacg gccctgaccc ccagcttccg ctccgaggcc ggcgcctcgg
      361 gtcgcgacac gcgcggcatg atccgccagc accagttcta caaggtcgag ctggtctcga
      421 tcaccacgcc ggaccagtcc gaagccgagc atgaacgcat ggtcggctgc gccgaggcgg
      481 tgctgaaggc cctgaacctg ccgttccgca ccatgctgct gtgcaatggc gacatgggct
      541 ttggcgccag gaagacctac gatctggaag tctggctgcc ctcgcagaac acctatcgcg
      601 agatcagctc gtgctccaac tgcggtgact tccaggcccg gcgcatggac gcccgcttcc
      661 gcaaggccgg cgagaagggc acccagtacg tccacaccct gaacggctcg ggcctggccg
      721 tgggccgcac cctggtggcc gtgctcgaga actatcagga cgagaccggc gggatcacca
      781 tccccgaggc gctggtcccc tatatgggcg gcgtcaccta tatcgggggt gaggggtgat
      841 cattatctcg ccaagccaaa ttggctgtca tcccggacga gcgcgcagcg tgaagatccg
      901 ggaccgacgc gtgaacgcct gcgcttccgg cggtcccgga tctgcgcttc gcttgtccgg
      961 gatgacaggc tcaaaatgag aatcctcctc accaacgacg acggcatcca cgccccgggc
     1021 ctcgtcgcgc ttgagaagat cgcccgcgcg ctcagcgacg acgtctggat ctgcgcgccg
     1081 gagtacgagc agtcgggggc cagccgggcc ctgaccttgt ctgatccgct gcgggtgcgc
     1141 cgcctggacg atcgccgctt cgccgtcgag ggcacgccca ccgactgcgt gatgatggcc
     1201 gtgcagcagc tgatcgaggg ccccgcgccg gatctggtgc tgtcgggcgt caatcgcggc
     1261 cagaacctgg ctgaagatgt caccctgtcg ggcaccgtgg ccggcgccat cgagggcatg
     1321 gccctgggca tccgctcgat cgccctgtcc caggcaatga actatttcca tgacgaggtt
     1381 gtcgcccact gggagacggc ccagacctat ggccccggca tcatccagcg tctgctggag
     1441 gtcggctggc cggccgacgt gatcatgaac atcaacttcc cgtctctgcc gccggaagag
     1501 gtgacggccg tcgaggtcac gcgccagggg ttccgcgacg gccacctgcg ccactgggac
     1561 aaacgcacgg atctgcgcgg ccgcgactac tattggatgg gcttcaccgc caaggcctcg
     1621 gaaccggccc cgggaaccga cctgaaggcg gtctatgagg gcaaaatctc ggtcacgccg
     1681 ctgcatatcg acctgacgca caatgagacc gtccacgcgc tgaagggcgt actgggcggc
     1741 gctccgccga agctgcgggt cgaggcatga ccggcaaggc caaggccgcg gcggccgccg
     1801 atctgccccg cctgatgcag gccctgcgcg accaggggat caccgatccc caggttctca
     1861 aggccatcga gaagacgccg cgcgatctgt tcacgcccga cctgttcaag gaacggtcgt
     1921 gggaagactc ggccctgccg atcgcctgcg gccagaccat cagccagccc tatatcgtcg
     1981 gcctgatgac ccaggccctg accatcgagc cgcgctcgcg cgtgctggag attggcaccg
     2041 ggtcaggcta ccagaccacc attctcagtc acgtgtcgcg gctggtctat acgatcgagc
     2101 gctatcgcac cctgatgaag gaagccgagg cgcgcttcac caagctgggt ctcaccaacg
     2161 tcatcaccaa gttcggcgac ggcggcgaag gttgggccga acaggcgcca tttgaccgca
     2221 ttatggtcac ggctgcggcc gaagatgatc cgaaacgact gctgtcccag ctcaagccca
     2281 acggcgtcct cgtggcgccg atcgggaagg ggccggtgca gagccttcgc cgctatgcgg
     2341 gcgacggcaa gggcggcttc aaggtcgagg cgctgtgcga cgtgcggttt gtaccgctgc
     2401 tcgatggcgt ggcccgcgac cagtgaagcg gaaatggggg cgaggcttgc gcggcgccct
     2461 tgcgattaat gattgattta ccctcgcggg gccgtctgga cgacttgcga ggaaaggaga
     2521 ggctatgagg cagttgtgga cgcgcgcggc ggtgatnnnn nnnnnngaac gctcgcggcc
     2581 tgcgagacga cctcgtcgcc gcagtatccc gcgcgcgaag ggcagcccgg tggatccccg
     2641 ccccgggccc agaccccgaa tttcccgatc acccaggcgc caccgtcgga agctgtgatc
     2701 gacactacgc ccgcgcccag cgaagtgatc cgccgcgaac cgatcccggt cgaggccgct
     2761 ccgccgccgc cgacctatag cgagccgccg gttacccgca ccgaactggc gccgcctccg
     2821 ccgcctccgc cgccagcgca gctccggccg gccgcgccga ccatcatcac caccgtgggc
     2881 ggacccgtgg tggacgtgcc cggcaagcct cagatgcgca tcgtcgagtc aggcgacgcc
     2941 ctggacgcca tcgcgcgcgg ctttggcacg acccgcgccg agctggtcaa gctcaacgac
     3001 cttgagccgc cctaccgcct gaagctgggc cagaagat
//
LOCUS       DAOMEO010000296         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3590_length_3035_cov_2.562081, whole genome shotgun sequence.
ACCESSION   DAOMEO010000296 DAOMEO010000000
VERSION     DAOMEO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_3035_cov_2.562081"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLH31_07775"
     CDS             complement(<1..193)
                     /locus_tag="PLH31_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89151.1"
                     /translation="MKILLVAGALTLAVFMSVGQPAKPLDDLRWQRDPTYLLAPYAAA
                     KIGRPETPRQIALASSTLQR"
     gene            317..1198
                     /locus_tag="PLH31_07780"
     CDS             317..1198
                     /locus_tag="PLH31_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR89152.1"
                     /translation="MRLPPFSTLTALEAAARHRSYSRAADELFVTHGAVSQQIRKLEE
                     DLGVRLFLRQGNRMEPTDTGAALAASVTEALASLKRGVQAARNAACGPIVLSTGAAFA
                     TRWLVTRLARISAETGELDLTVRVEDRVSDLSSDGADVALRFGVGPWPGLESVRLLNE
                     RLFPLCSPALLERHPINKPEDLLNAPLLRHTALPWSAWFQAMGLPAPDLPPSLGFDDT
                     SMMLDAAAQGLGVALARSGLSERDIRDGRLVRPLAGEVEVDTGHHFVWRADNPKLPRL
                     LKLRDWFVAETANSRGQ"
     assembly_gap    1413..1422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1423..1953)
                     /gene="efp"
                     /locus_tag="PLH31_07785"
     CDS             complement(<1423..1953)
                     /gene="efp"
                     /locus_tag="PLH31_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQR89153.1"
                     /translation="MKVAASSLRKGAVVDMDGKLYVVLTVDNIHPGKGTPVTQLNMRR
                     ISDGVKVSERYRTTEQVERAFVDQRDHTFLYQDAEGFHFMNPENYDQVVATPDVIGDA
                     APYLQENMTVQLSTHNDIPIAIELPRTMILEVVDTEPSVKGQTASSSYKPALLSNGIK
                     TTVPPYIAVGVRVVILT"
     gene            2142..>3035
                     /gene="epmA"
                     /locus_tag="PLH31_07790"
     CDS             2142..>3035
                     /gene="epmA"
                     /locus_tag="PLH31_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HQR89154.1"
                     /translation="MVRNRITRAVRGWFEDQDFVEVEAAALQVSPGNEAHLHAFQTTM
                     LSLAGEPDQTFYLHTSPEFACKKLLAAGEAKIFDFARVWRNRERGPLHHPEFVMLEWY
                     RAGAPYQTLMDDCAVLLSLAAETAGTRRFSFRGLECDPHQPPERLTVAEAFERHAGVD
                     LLASLGPGGGTDRDTLAASAKAAGIRVADDDSWADVFSKVIVEKVEPNLGQGHATILC
                     EYPVAEAALARPKPGDPRVAERFELYACGVELANAFGELTDPAEQRRRFAAEMDEKAR
                     IYGERYPIDEDFLAALEIMPEA"
BASE COUNT          463 a          998 c         1036 g          528 t
ORIGIN      
        1 ggcgctgcag ggtcgacgag gccagggcga tctggcgcgg tgtctccggt cgaccgatct
       61 tcgccgccgc gtaaggcgcc agcagatagg tcggatcgcg ttgccagcgc agatcgtcga
      121 gcggtttggc cggttggccg acgctcatga atacggcgag cgtcagcgcg cccgcaacaa
      181 gcaggatctt catttcgtca ccttgcggag aggggtggtg gaaagagcgg cgccgagggg
      241 acaatcgctc cagagcctcc cggaaaccaa cgatctctat tggtccagct tgtgagctgc
      301 actaacatct ctgcccatgc gcctgccccc cttctcgacc ctgaccgccc tggaagccgc
      361 cgcgcgtcat cgcagctaca gccgcgcggc cgacgagctg tttgtgaccc atggcgcggt
      421 cagccagcag atccgcaagc tggaggagga ccttggcgtt cgcctgttcc tgcgtcaggg
      481 caatcgcatg gagcccaccg acaccggggc ggccctggcc gcgagcgtga ccgaggcgct
      541 ggcaagcctg aagcgcggtg ttcaggccgc ccgcaacgcg gcctgtgggc ccatcgtact
      601 cagcacgggc gcggccttcg ccacgcgctg gctggtcacg cgcctggccc gcatctcggc
      661 cgagacgggc gaactggacc tgacggtccg ggtggaggac cgggtgtctg acctgtcttc
      721 agatggcgcc gacgtcgccc tgcggtttgg cgttggcccc tggccgggac tggaatcagt
      781 gcgcctgctc aatgagcgcc tgtttcccct ctgcagtccc gctctgctgg agcggcaccc
      841 gatcaacaag cccgaggacc tgctgaacgc gcccctgctg cgccataccg ccctgccctg
      901 gagcgcctgg ttccaggcca tgggcctgcc agcccccgac ctgccgccca gcctgggctt
      961 tgacgacacc tcgatgatgc tggacgccgc cgcccagggc ctgggcgtcg cgctggcccg
     1021 tagcggcctg tccgagcgcg acattcgcga cggccggctg gtgcgacccc tggcgggcga
     1081 ggtcgaggtc gacaccggcc accacttcgt ctggcgcgcc gacaatccca agctgccgcg
     1141 cctgctcaag ctgcgcgact ggttcgtggc cgagacagcg aacagtcggg gccagtgagg
     1201 tcggccgggg gctggcgctt cgctgcgcta cggccgggat gacaagattt tttgggctag
     1261 ctggaagtct gagacgcggg cctttcgaac gtccgactag gatcgtgtgg cccagcgaaa
     1321 aaggcccggc gcttacgcac cgggcctcct gatctcgcca ttctcccaca aggggagaag
     1381 gaaaaccgtc cctagtcctt ggcgcgttcc tgnnnnnnnn nncgtcagga tcaccacccg
     1441 cacgcccacg gcgatatagg gcggcacggt ggtcttgatg ccgttgctga gcagggccgg
     1501 cttgtacgac gagctggcgg tctggccctt caccgacggt tcggtgtcga ccacttccag
     1561 gatcatggtg cgcggcagtt cgatggcgat cgggatgtcg ttgtgggtcg acagctggac
     1621 cgtcatgttc tcctgcagat agggggcggc gtcgccgatc acgtccgggg tggccacgac
     1681 ctggtcgtag ttttccgggt tcatgaagtg gaagccttcg gcgtcctgat acaggaaggt
     1741 gtggtcgcgc tggtcgacaa aggcgcgctc gacctgctcg gtggtgcggt agcgttccga
     1801 aaccttcacg ccgtcgctga tgcggcgcat gttcagctgg gtcaccgggg tgcccttgcc
     1861 ggggtggatg ttgtcgacgg tcagaacgac atagagcttg ccgtccatat cgacgacggc
     1921 gcccttgcgg agcgagctgg cggctacctt cacttcgtag gtccttggct tttccgcgcg
     1981 tccagacgaa ccagcgcgaa gggggtggtg atcctgtgac cgccctccta tagcgaacgc
     2041 ggcaagatcg ccagtctcta ctacatggag gccctgtggc tgcagacgcc ccttctccct
     2101 ggtggacgcc acaggtccat gcagaccgcc ggccgttcct gatggtccgc aaccggatca
     2161 cccgggcggt gcgcggctgg ttcgaggatc aggacttcgt cgaggtcgag gccgccgccc
     2221 tgcaggtctc gcccggtaac gaggcccatc tgcacgcctt ccagaccacg atgctgagcc
     2281 tggccggcga gccggaccag accttctatc tgcacacctc gccggaattt gcctgcaaga
     2341 agctgctggc ggcgggcgag gccaagatct tcgacttcgc ccgggtgtgg cgcaatcgcg
     2401 agcgcgggcc gctgcaccat cccgagttcg tgatgctgga atggtaccgg gccggcgcgc
     2461 cgtaccagac cctgatggac gactgcgcgg tgctgctgtc gctggcggcc gagacggcgg
     2521 gaacccggcg tttttccttt cgcggcctgg agtgcgatcc gcatcaaccg cccgagcggc
     2581 tgaccgtcgc cgaggctttc gagcgtcatg ccggtgttga tttgctggcg agtctgggcc
     2641 ccggcggcgg gacggatcgc gatacgctgg ctgcgtccgc caaggcggcg gggattcgcg
     2701 tggccgacga cgacagctgg gccgacgtct tcagcaaggt gatcgtcgag aaggtcgagc
     2761 ccaacctcgg ccagggccac gccacgatcc tgtgtgagta tcccgtggct gaggccgccc
     2821 tggcgcggcc caagccgggc gatccgcgcg tggccgaacg cttcgagctc tatgcctgcg
     2881 gggtcgagct ggccaacgcc tttggcgaac tcaccgaccc cgccgaacag cgccgccgct
     2941 tcgcagccga gatggacgag aaggcgcgga tctatggcga gcgctatccg atcgacgagg
     3001 atttcctggc ggcgctggag atcatgcccg aggcg
//
LOCUS       DAOMEO010000297         3029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3599_length_3029_cov_3.403497, whole genome shotgun sequence.
ACCESSION   DAOMEO010000297 DAOMEO010000000
VERSION     DAOMEO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3599_length_3029_cov_3.403497"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..329)
                     /locus_tag="PLH31_07795"
                     /pseudo
     CDS             complement(1..329)
                     /locus_tag="PLH31_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090936.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            383..766
                     /locus_tag="PLH31_07800"
     CDS             383..766
                     /locus_tag="PLH31_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR89155.1"
                     /translation="MDVHKHSAFERLEIVETGRRRRWSEDEKLRIVMESQSAPRLVSA
                     TARRHGISRSQLGTWLRSFRTEQPATKPEPAFVPVLMAPGAMSDPFAAADDTAIVVDL
                     PGGRRLRIAASTPPALAAAVLRALR"
     gene            763..1104
                     /gene="tnpB"
                     /locus_tag="PLH31_07805"
     CDS             763..1104
                     /gene="tnpB"
                     /locus_tag="PLH31_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HQR89156.1"
                     /translation="MIPSRARVWIAIGHTDMRRGMQGLSLLVQEGLKRDPNGGDLFVF
                     RGRSGSLVKILWHDGVGLSLYAKRLERGRFVWPSAKDGVVALTHTDLACLLDGIDWRN
                     PQRTWRPEAVG"
     gene            1223..2135
                     /locus_tag="PLH31_07810"
                     /pseudo
     CDS             1223..2135
                     /locus_tag="PLH31_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     assembly_gap    2136..2145
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2146..2834
                     /locus_tag="PLH31_07815"
     CDS             <2146..2834
                     /locus_tag="PLH31_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HQR89157.1"
                     /translation="ILQADAYSGYGRLYAEDRSPRPLIQALCWSHAKRKFFELADIAK
                     NARRGKNAASISPMALEAVKRIDALFAIEREANGLHAAERLAIRRERSLPLLADFENW
                     MRTERARLSRHADVAKAMDYMLTKWDAFARFTTDGRICLSNNAAERALRGIALGRRSW
                     TFAGSKRGADRCAFMLTMIATAKLNDVDPQAWLADVLARIADMPQSRLPELLPWNWAA
                     APHQDQAVAA"
     gene            complement(2860..3029)
                     /locus_tag="PLH31_07820"
                     /pseudo
     CDS             complement(2860..3029)
                     /locus_tag="PLH31_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          505 a          997 c         1024 g          493 t
ORIGIN      
        1 cccttgtgga agatctcgat ggtccgatcg gcgatgcgga catcgacccg ttcgcggatc
       61 aggcgatagg gaaccgagta ccagcagccg tcgacttcga catggtaatc gggagcgacc
      121 cggcagtgct tccacagggc gaaggcatag ggctcggccg gcaacggccg cagcgccggg
      181 gcatcgatcg tcgcgaacaa ctcggcgcgg ctggcgccgt agccgcgcat gacccgggcg
      241 ttgagttccc cgaccagctc ggcgatcgcc aggttgagtt cgacccggga gaagaagcgc
      301 cggttgcgca gccgcgccag gaccgtacgc ctacggtcta aaccgccttt cgaactgacc
      361 ggcgcggtaa gagactgcgc gtatggacgt ccataagcac agtgccttcg agcgtttgga
      421 gatcgtggag acgggtcgtc gtcgccgttg gagcgaagac gagaagctcc ggatcgtgat
      481 ggagagccag tcggcaccgc gtctggtgtc ggcgacggcg cggcgtcacg ggatttcgcg
      541 ctcccagttg gggacgtggc tgcgcagctt tcgcaccgag cagcctgcca caaagccgga
      601 gccggccttc gttccggttc tgatggcccc tggggctatg tccgacccct ttgcggccgc
      661 cgatgacacg gcgatcgtag tggacctgcc gggtggccgt cggctgcgga ttgccgcctc
      721 gacgccgccg gcgctggcgg ctgcggtcct gagggcgctg cgatgatccc gtcgcgggcg
      781 cgggtctgga tcgcgatcgg tcacaccgac atgaggcggg gcatgcaggg tctgtcgctc
      841 ctcgtccagg aggggttgaa gcgcgatccg aacggtggcg atctcttcgt ctttcggggc
      901 cgcagcggat cgctggtgaa gatcctctgg catgacgggg ttgggctgtc gctttacgcc
      961 aagaggctgg agcgcggacg cttcgtctgg ccctcggcca aggacggcgt cgtggctctg
     1021 acccacacgg atctcgcctg cctgctcgac gggatcgact ggaggaaccc gcagaggacg
     1081 tggcgtccgg aagcggtggg atagcgcaca aaagtctttg cattgcaggg cttgcgtgac
     1141 agaatctgtc tgtcgcgaac gagactttcg agcgctgtgc atggccgatc tgccggaaga
     1201 catcaccgcc ctgaaggccg cgctggcggc atcggaacgg cgtgccgact tggccgagat
     1261 cgagcgggac gaggcgctcg cggatgcggc caatgcgaag gcgaaggcct ccggcatcga
     1321 agccctcgtc gcccatctga cgctgcggat cgagaagctg aagcgcgagc tctacggcac
     1381 tcgctccgag cgcacggccc gacttctcga tcagttggag atgcagcttg aggacgccga
     1441 ggcggctctg agcgaagacg aactcgccgc agagcatgcc gcggtgaaga cgacgacggt
     1501 ggcctccttc gagcgccgtc gccgcggcgg ccgcaagccg ttccccgagc atctgccgcg
     1561 cgagcgcgtc gtggtacccg gtccctccgc atgctcctgc tgcggctcgg atcgcctgcg
     1621 caagcttggc gaggacgtca ctgagacgct ggaggcgatc ccccgccggt ggaaggtgat
     1681 ccagacggta cgcgagaagt tcacctgccg ggactgcgaa cggatcagcc aggcgccggc
     1741 accgttccac ccgaccccgc gagggttcct cggccccaac ctcctggcga tgatcctgtt
     1801 cgacaagttc ggacaacacc agccgctgaa ccggcagtcg gagcgctatg cccgtgaggg
     1861 gatcgagctg tcgctgtcga cgctggccga ccaggtaggc gcctgcgctc atgtccttca
     1921 gccgctgcac gacctcattg ccgcccacgt cctcgccgcc gagcgcctgc atggcgacga
     1981 tacgacggtg ccgatcctgg ccaaggggaa gaccgtgacg ggccggatct ggacctatgt
     2041 gcgcgacgat cgtcccttcg acggccaggg cccgccggcg gccctatact atgcctcccc
     2101 ggaccgcacg gccgagcaac cccaagagca tctggnnnnn nnnnngcatt ctccaggccg
     2161 atgcctactc gggctatggt cgcctctatg ctgaggatcg aagtcccaga ccgctcatcc
     2221 aggcgctttg ctggagccat gccaaacgga agttcttcga gctcgccgac atcgcgaaga
     2281 atgcccggcg cggaaagaac gcggcgtcga tctcgccgat ggcgctggag gcggtcaagc
     2341 gcatcgatgc actgttcgca atcgagcgcg aggcgaacgg tctgcatgcc gccgagcgtc
     2401 tggcgatccg ccgcgaacgc tccctgccgc tcctcgccga cttcgagaac tggatgcgca
     2461 ccgaacgggc gcgcctgtcg cgccacgccg acgtcgccaa ggcgatggac tacatgctga
     2521 ccaagtggga cgcctttgcc cgcttcacga ccgacgggcg catctgcctg tcgaacaacg
     2581 ctgcggagcg agccctgcgc ggcatcgccc ttggccgccg ctcgtggacc ttcgccggtt
     2641 cgaagcgcgg cgccgatcgc tgtgccttca tgctgacgat gatcgccacc gccaagctca
     2701 atgatgtcga tccgcaggcc tggctcgccg acgtcctcgc ccgcattgcc gacatgcccc
     2761 aaagccggct gcctgaactc ctgccatgga attgggccgc ggcaccccat caggatcaag
     2821 ccgtagctgc ctgactgcgg cctacgccgg atgcgtaccc aggacccagc gctcgaccag
     2881 caagaccgac tgctccacct tggccttgtc ccggggtttg tagggtcggg cgggaaggac
     2941 agtggcgccg taatggcggg ccatgtcctg gtagcgtacg catacgacga tggccgcggg
     3001 ataggtgccc gcgcgagcga tgttggcgc
//
LOCUS       DAOMEO010000298         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3600_length_3028_cov_3.061554, whole genome shotgun sequence.
ACCESSION   DAOMEO010000298 DAOMEO010000000
VERSION     DAOMEO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3600_length_3028_cov_3.061554"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLH31_07825"
     CDS             complement(<1..270)
                     /locus_tag="PLH31_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670072.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HQR89158.1"
                     /translation="MAEAFICDAIRTPIGRYAGALSSVRADDLAAITLKALAARNTGV
                     DWSLVDDVVLGCANQAGEDNRNVARMAGLLAGLGTEASGSTINRLC"
     gene            403..1977
                     /locus_tag="PLH31_07830"
     CDS             403..1977
                     /locus_tag="PLH31_07830"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQR89159.1"
                     /translation="MINRRDMMGGAVVGAALLTAGGAQARISGDGPLRTLLDKIAKGG
                     PAVERLAVLKDFRPANLSPVAQLDFDAVLQSLETEAEIVRRFPFGTGASSPYVVTTRS
                     GVWLKAVDAAKSADAAADAVKRLDTETARIAEDAAKGVILPAFVIDKVVAGLAQIAKA
                     VEAAPDLAAALGRQHDALIALRSQAGKGIGVHRFKDGEAYYALLLKAAMGADITPAQA
                     WDRADAAAKALTARAETLLTAQGLREGTVGARLAALSLDERWLYTDDDAGRDRLTADM
                     NAWLARAVARLPMSFATVAPGSRAVSMRRMSPADEAARRQGYREAPSFDGSRPGVYYA
                     DLNTIRTRPSWTLPSVVHHESVAGHMVQMPLEEGSGAHPLRSRIAFPGFSEGWAIYAE
                     QLADEEGAFAGDPLAQLGYIQWMLFRVGRLLADLGIHLKGWSVEKTTAFLNDLQGPPP
                     VFSPVSQGIERIAIGPAASAGQGLAWLELVRLREAARKLSGSGFDIRAFHDAVLAQGT
                     LPLSMLRARVLGASAA"
     gene            complement(2786..>3028)
                     /gene="ruvX"
                     /locus_tag="PLH31_07835"
     CDS             complement(2786..>3028)
                     /gene="ruvX"
                     /locus_tag="PLH31_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQR89160.1"
                     /translation="GVRCQSNRALGRNLLRLQPHVPITFWDERLSTAAVTRVLIEEHD
                     VNRKRRAEVVDKMAAGWILQGALERMRGIREAAGKV"
BASE COUNT          455 a         1051 c         1052 g          470 t
ORIGIN      
        1 gcacagacga ttgatggtcg agccggaggc ttcggttccg aggcctgcca gcagcccggc
       61 catgcgggcg acgtttcggt tgtcctcgcc ggcctggttg gcgcagccca gcaccacgtc
      121 gtcgaccagg ctccagtcga cgccggtatt gcgcgcggcc agggccttca gcgtgatcgc
      181 cgccaggtcg tcggcccgca ccgacgacag cgccccggca tagcggccga tgggggtgcg
      241 gatcgcgtcg cagatgaagg cttcggccat ggcggatctc tcaaacgatt gttcgccgca
      301 ccctagcggc cgggcggaca cgatcaagcc gccgaatggc tggacaccgg tgtcagatcg
      361 cctagacctg agcccaacga gagacttccg ggagaagacg ccatgatcaa ccggcgcgac
      421 atgatgggcg gggccgtggt cggcgcggcc ctgctgacag cgggcggcgc gcaggcgcgg
      481 atctcgggcg atggacctct gaggaccttg ctggacaaga tcgccaaggg cggcccggcc
      541 gtcgagcgcc tggcggtgct gaaggacttc cggcccgcta acctgtcgcc ggtcgcgcaa
      601 ctggacttcg acgccgttct gcaatcgctg gagaccgagg ccgagatcgt ccgacgcttc
      661 ccgttcggaa cgggggccag ttcgccctat gtggtgacca cccgctcggg cgtctggctg
      721 aaggcggtcg atgcggccaa gtccgccgat gcggccgctg acgctgtcaa acgccttgat
      781 accgagaccg ccaggatcgc cgaagacgcc gccaagggcg tgatcctgcc cgcctttgtc
      841 atcgacaagg tcgtcgcggg tctggcccag atcgccaagg ccgttgaagc cgcgcctgac
      901 ctggccgccg ccctgggccg ccagcatgac gccctgatcg ccctgcggtc ccaggccggc
      961 aagggcatcg gcgtgcatcg gttcaaggac ggcgaggcct attacgccct gctgctgaag
     1021 gccgccatgg gcgcggacat tacgccggcc caggcctggg accgcgcaga cgccgccgcc
     1081 aaggccctga ccgcccgcgc cgagacgcta ttgacggccc aaggtctgcg tgagggaacg
     1141 gtcggcgctc gcctggccgc cctcagtctc gacgaacgct ggctctacac cgatgacgac
     1201 gccggccgcg accggctgac ggccgacatg aacgcctggc tggccagggc cgtcgcgcgc
     1261 ctgccgatgt cgttcgccac ggtcgcgccg ggcagtcgcg cggtcagcat gcgccgcatg
     1321 tcccccgccg acgaggccgc tcgccgccag ggctatcgcg aggcgccgtc gttcgacggc
     1381 tccagaccag gcgtctacta cgccgatcta aacaccatcc gaacccggcc cagctggacc
     1441 ctgcccagcg ttgtccatca cgagagcgtg gcgggccaca tggtgcagat gccgctggag
     1501 gagggctccg gagcacaccc gctgcgcagc cgtatcgcct ttccgggctt ctccgaaggc
     1561 tgggcgatct atgccgaaca gctggccgac gaggagggcg ccttcgcggg cgatccgctg
     1621 gcccagctgg gctatatcca gtggatgctg tttcgcgtcg gccgcctgct ggccgatctg
     1681 ggcatccacc tgaagggctg gagcgtcgag aagaccacgg ccttcctgaa cgacctgcag
     1741 ggcccgccgc cggtgttctc gccggtgtcg caagggatcg agcgcatcgc cattggcccg
     1801 gcggcttcag cggggcaggg tctggcctgg ctggaactgg ttcgcctgcg tgaggcggcg
     1861 cgcaagctgt cgggctcggg cttcgacatc cgcgctttcc acgacgcggt gctggcgcag
     1921 gggaccttgc cgctgtcgat gctgcgggcg cgggttttgg gcgcgtcggc tgcctgagcg
     1981 cggggatttc gatcgcgccg tccccggatg ggcgcgggga tgagtgatcc aagtcctccc
     2041 cctgaagggg gagcatcatg agccgcccat acaatccccg tcatcccggg acttgttccc
     2101 gggacccatc gttccgttgc gccgtaaggg gtttgtgatc caccgaccgt gcggccgacc
     2161 catggatccc ggggacgagc cccgggatga cgagcttttt cgagtgtcac gctcccgggc
     2221 gccgacgcgg gcgtccagcc aatcgatcag acgcgcgcaa acctcccgta ttctatcccc
     2281 acctcgtcgc gaggaaaggg cgcaaggcca gcgaccattg cggcggcggg gggtgatcga
     2341 gcgggactgg ggtcgagggg gatactcgac cggtccgggg atcccggcgt tgcgggaccc
     2401 gcccgccgtc ggcgtggacg gagagacccg cgggagatgc ctaaggccct cgcgcttcga
     2461 ccgccgggcc gctctggaaa cggagcggtt cgggttctgc taaggcctca acagggccac
     2521 ctggccggac gctggcggat aacgatgacg cggagcgctc ggcttcgttg aaacggttat
     2581 tctatctctc atccggacgg ggtccggacg ctccgcgacc ctttccatca actcggcggt
     2641 ttccgcgcga gatcgcggcc ccgtagctgc tcccccgctg ggggagctgt cgcggagcga
     2701 ctgagggggc taatgcggcg ttcgccccgc gggccccctc cggccctccg ggccacctcc
     2761 cccagagggg gaggagctaa gaaagctaaa ccttgcccgc agcttcccgt atcccccgca
     2821 tccgctccag cgcgccctgc aggatccagc cggcggccat cttgtcgacc acctcggcgc
     2881 ggcgcttgcg gttgacgtcg tgctcctcga tcagcacccg cgtgacggcg gcggtcgaca
     2941 gccgctcatc ccagaaggtg atcggcacgt gcggctgcag gcgcagcagg ttgcgaccca
     3001 gcgcccggtt ggactggcaa cgaacgcc
//
LOCUS       DAOMEO010000299         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3613_length_3021_cov_2.600809, whole genome shotgun sequence.
ACCESSION   DAOMEO010000299 DAOMEO010000000
VERSION     DAOMEO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3613_length_3021_cov_2.600809"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1605
                     /gene="fumC"
                     /locus_tag="PLH31_07840"
     CDS             214..1605
                     /gene="fumC"
                     /locus_tag="PLH31_07840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQR89161.1"
                     /translation="MTATRIETDTFGPIEVAADRYWGAQAQRSLGNFKIGWEKQPLPV
                     VRALGIVKRAAAEANLALGKLDPKIAETIVAAANEVIDGKLNDHFPLVVWQTGSGTQS
                     NMNANEVISNRAIEMLGGEMGSKKPVHPNDHVNMSQSSNDTFPTAMHIACAEQVVHDL
                     IPALKHLHKALAAKAAAWKDIIKIGRTHTQDATPLTLGQEFGGYAQQIENGIARIEMT
                     LPMLMQLAQGGTAVGTGLNAPVGFAELVAEKIAGVTGLPFTTAPNKFEALAAHDAMVF
                     THGAINTVAASLFKIANDIRFLGSGPRAGLGELALPENEPGSSIMPGKVNPTQSEALT
                     QVCAQVFGNHSTLTFAGSQGHFELNVFNPVMAYNFLQSVRLVADAAISFTDNCVVGIE
                     PRVDNIRKGVENSLMLVTALNGKLGYDACAKIAKTAHKNGTTLREEAVGGGYLTNEEF
                     DEYVRPEKMISPG"
     gene            1750..2520
                     /locus_tag="PLH31_07845"
     CDS             1750..2520
                     /locus_tag="PLH31_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR89162.1"
                     /translation="MTALDRPYWIEALIQRREGWDPEAYPFNLPVLRGLESIAFHPRV
                     TFLVGENGSGKSTLIESLAVAWGFNAEGGSRNFNFSSRASHSELHSYIRPVRSIIRPH
                     DGYFLRAESFFNVQTEIEALDREPGPGGSFIASAYGARPLHEQSHGEAFFALFENRFR
                     GDGLYILDEPEAALSPSRQLSFLAKLHQLVLARSQLIIATHSPIILGYPDAWIYETSE
                     RGLERVEYEDLDHVTVTRSFLNRRQTFLDVLLSEDDEE"
     gene            2535..2777
                     /locus_tag="PLH31_07850"
     CDS             2535..2777
                     /locus_tag="PLH31_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HQR89163.1"
                     /translation="MDAPELDLGIDPELLAQARRLGISVAGLSETQLRLHLQKVDPAG
                     AEERARRWAAENAEAIAEHNRFVEEHGLLSDHLRTW"
     gene            2778..>3021
                     /locus_tag="PLH31_07855"
     CDS             2778..>3021
                     /locus_tag="PLH31_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89164.1"
                     /translation="MRQFDLCENPATPMRAGAPFVVVLSSHLLGDLIEAVVAPVRRGA
                     SAGIPGLEVPVVIEGETLLVSVSGVAGIRATALRNRK"
BASE COUNT          560 a         1037 c          920 g          504 t
ORIGIN      
        1 gttatcggcc acctcgcgca gttccttcgt cgtgaccttg cggccgccga cattaacggc
       61 gcgcaagagg gcgatgtacg ggctcatggg tgtctccaac cagccggatc ggcgagccgt
      121 aaggcccggc gcgccatatc gcaaccgcga acgttgccct gggctcgcct tgggtctaga
      181 ccgcgctcga ccgtttttcc ggagacccgc cgcatgaccg ccacgcgcat cgaaaccgac
      241 accttcggcc ccatcgaagt cgccgccgac cgctactggg gcgcgcaagc ccagcgcagc
      301 ctgggcaact tcaagatcgg ctgggaaaag cagcccctgc cggtggtgcg ggccctgggt
      361 atcgtcaagc gcgccgccgc cgaggccaat ctggcgctgg gcaagctgga tccgaagatc
      421 gccgagacca tcgtcgccgc cgccaatgaa gtgatcgacg gcaagctgaa cgaccacttc
      481 ccgctggtgg tctggcagac gggttccggc acccagtcga acatgaacgc caatgaggtg
      541 atctccaacc gcgccatcga gatgctgggc ggcgagatgg gcagcaagaa gcccgtccac
      601 cccaatgacc acgtcaatat gagccagtcg tcgaacgaca ccttcccgac ggccatgcac
      661 atcgcctgcg ccgagcaggt ggtccacgac ctgatccctg ctctgaagca cctccacaag
      721 gccctggccg ccaaggccgc cgcgtggaag gacatcatca agatcggccg cacccacacc
      781 caggacgcca cgcccctgac gcttggccag gagttcggcg gctacgccca gcagatcgag
      841 aacggcatcg cccgcatcga gatgaccctg ccgatgctga tgcagctggc gcagggcggc
      901 acggccgtcg gcaccggcct gaacgctccg gtcggctttg ccgaactggt cgccgagaag
      961 atcgccggcg tgaccggcct gcccttcact accgccccca acaagttcga ggccctggcc
     1021 gctcacgacg ccatggtctt cacccacggc gccatcaaca cggtggccgc gtcgctgttc
     1081 aagatcgcca acgacatccg cttcctgggc agcggccccc gcgccggcct gggcgaactg
     1141 gccctgccgg agaacgagcc cggctcgtcg atcatgccgg gcaaggtcaa tccgacccag
     1201 tccgaagccc tgacccaggt ctgcgcccag gtgtttggca accactccac cctgaccttc
     1261 gctggcagcc agggccattt cgagctgaac gtcttcaacc cggtgatggc ctacaacttc
     1321 ctgcagtcgg tgcgcctggt ggccgacgcc gcgatcagct tcaccgacaa ctgcgtcgtc
     1381 ggcatcgagc cccgcgtcga caacatcagg aagggcgtcg agaacagcct gatgctggtc
     1441 acggccctga acggtaagct gggctacgac gcctgcgcca agatcgccaa gaccgcgcac
     1501 aagaacggca ccaccctgcg cgaggaagcc gtcggcggcg gctatctgac gaacgaggaa
     1561 ttcgacgagt acgtgcgtcc ggagaagatg atctcgccgg gctgacgcct tcgaccacac
     1621 agttgatctg acaatccacc ggaggcctcc gcgctgtcgt cgccgaagcc cccggtgcgt
     1681 tcttgccgac ttgcgcttgc aaacagcgcc cgatgtgcaa tcttgaatag aacatatcgg
     1741 gaactagaga tgaccgcgct cgaccgcccc tattggatcg aagccctgat ccagcgccgt
     1801 gagggttggg atccggaagc ctatcccttc aacctgccgg tgttgcgcgg gctggaaagc
     1861 atcgcctttc atccgcgcgt caccttcctg gtcggcgaga acggttcggg caaatcgacc
     1921 ctgatcgagt cgcttgcggt ggcctggggc ttcaatgccg agggcgggtc gcggaatttc
     1981 aatttcagca gccgcgcgtc gcattccgag cttcacagct acatccgccc cgtacgatcg
     2041 atcatccgtc cgcacgacgg ctacttcctg cgcgccgaga gtttcttcaa cgtccagacc
     2101 gagattgaag cgctcgaccg ggagcccggc ccgggcggat ccttcatcgc cagcgcctat
     2161 ggcgctcgcc ccctgcacga gcagtcgcat ggcgaggcct tcttcgccct gttcgagaac
     2221 cgttttcgcg gcgatggcct ctacatcctc gacgaacccg aagccgccct gtcgcccagc
     2281 cggcagttgt cgttcctggc caagctgcat cagctggtgc tggcccgctc gcagctgatc
     2341 atcgccaccc attcgccgat catcctgggc tatcccgacg cctggatcta cgagacctcc
     2401 gaacgcgggc tggagcgtgt ggagtacgaa gacctcgacc acgtgacggt gacgcgcagc
     2461 ttcctcaacc gccgccagac cttcctggac gtgctgctgt cggaggatga cgaggaatga
     2521 taatctcccg ccctatggac gcccccgaac tagacctcgg cattgatccg gaactgttgg
     2581 ctcaggcccg acggctaggg atttccgtgg cgggactcag cgaaacccag cttcgcctgc
     2641 atctgcaaaa ggtcgatccc gccggcgcgg aggaacgggc tcggcggtgg gcggcggaga
     2701 acgctgaagc gatcgccgag cacaaccgtt tcgtagaaga gcacgggctc ctgtcggacc
     2761 atttgcggac ctggtgagtg cggcagttcg atctctgcga aaatccggca acgcccatgc
     2821 gagcaggcgc gccgttcgtc gttgtgttgt cctcacatct gctgggcgat ctgatcgaag
     2881 ccgttgtcgc accggtcagg cgcggcgcca gcgccgggat accgggcttg gaagtgccgg
     2941 tggtaatcga aggagaaacg ctgctcgtta gcgtatcggg cgtcgccggg attcgggcta
     3001 cagcgcttcg caaccgcaaa g
//
LOCUS       DAOMEO010000300         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3621_length_3014_cov_2.393038, whole genome shotgun sequence.
ACCESSION   DAOMEO010000300 DAOMEO010000000
VERSION     DAOMEO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_3014_cov_2.393038"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1130)
                     /locus_tag="PLH31_07860"
     CDS             complement(75..1130)
                     /locus_tag="PLH31_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQR89165.1"
                     /translation="MSSNHKDFGVFLPVANGGWIVSGTTPRIDGLYPQNRAAAVLADE
                     IGLDFIMSMAKFRGFGGTTDHWGTVLESVTLMAGLAEATKRVKIWATLHPLLQNPAVA
                     AKMIATLDHISGGRAGLNIVAGAYREEFAQMSAWDEALDHDDRYALAEEWTHVVKRLW
                     SEDRVDHHGRFFDLTDCQSDPKPLSRPRPDLICAGSSPRGFAFAVAEADACFIGGATT
                     EQRRDASRRAKAMAQGLGKSIKTYAMCTVIHADTDAAAHALAQTYADGVDLDAVRTML
                     RSWGAPADKLDAAAKQQGAFMTETVIGSPATCGDRIAAFVEDCELDGLMLIFPDYVAG
                     LTMFGEAILPGLRRRFA"
     gene            complement(1185..2474)
                     /locus_tag="PLH31_07865"
     CDS             complement(1185..2474)
                     /locus_tag="PLH31_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89166.1"
                     /translation="MASTDLRRISLLAGFSLLLFLITASTYGSLGVVLPAMIGELGWS
                     FEKAFLGFSVLGVFTGASSWLPAILIRRIGVRGTLLLGSAVLGAGLLGFAGSESIARY
                     YVGAALCGIGFQMAALIPGTHVLSALYRKRALPFGIYFTFGSLGGVAGPWMALMIMGA
                     TDDNWRIYWMVQAVLACVVGVICAALVGGSKWLAQAAHELDVEVEQAARAAPADARVY
                     RTAREWTVRAALRTPQFYVLVAAYFTHLLTGVTVASVSVSHIAEIGVATGMAAAAAAA
                     IGAKMLSLESLMQTLARLGGGFIGDRIDPKWLLVGAQAMLVAGLVALGAATTLPMMLL
                     YAVGTGVGFGLTVLAVTVLLLNYYGRRSNLELFSLTCLVGAVSAAGPFIAGAMRDRLG
                     SFTPTFQLFAAVIAVVFVAALFMRPPTPPAGMIDVAE"
     gene            complement(2477..>3014)
                     /locus_tag="PLH31_07870"
     CDS             complement(2477..>3014)
                     /locus_tag="PLH31_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89167.1"
                     /translation="KGVELEVRWLASEQLSFTFAGNSQRTTIKGPDASFQYIPAYTAG
                     VPGANAYGGSYVVWSFASLPGRGGDYAYTLIPKSVVSLYGAYTSKAYDWGQAGATLGV
                     SHVSRTAGTVQNAVRYPAYSVVNGSAHVTRGPYTAELNVDNLFDKFHFTPDADTYANL
                     GALPGKGREWRVTLKRTF"
BASE COUNT          548 a         1045 c          988 g          433 t
ORIGIN      
        1 atccacctgc atgtcgacga tcaacagggc cgtgcgcccc ggcgcgatcc agtcaggctc
       61 cagcgggaca tcgctcatgc gaaacgccgc ctcagtcccg gaaggatcgc ctcgccaaac
      121 atggtcaggc cggcgacata gtcggggaag atcagcatca ggccgtccag ttcgcaatcc
      181 tcgacgaagg cggcgatcct gtcgccgcag gtcgccggcg aaccgatcac cgtctcggtc
      241 atgaacgccc cctgttgctt ggcggccgcg tccagcttgt cggccggcgc cccccagctt
      301 cgcaacatgg tccgcaccgc atccagatca accccgtcgg cataggtctg ggccagggca
      361 tgggccgccg cgtcggtgtc ggcatggatg accgtgcaca tggcgtaggt cttgatcgac
      421 ttgcccagac cctgggccat ggccttggcg cggcggctgg cgtcgcggcg ctgttcggtg
      481 gtggctccgc cgatgaaaca ggcgtcggcc tcggctacgg cgaaggcgaa accgcgcggc
      541 gagcttcccg cacagatcag gtcgggccgg ggccgcgaca gcggcttggg atcggactgg
      601 cagtcggtca ggtcgaaaaa gcggccatgg tgatcgaccc ggtcctcgct ccacagccgc
      661 ttgaccacat gggtccattc ctcggccagg gcgtagcggt catcgtgatc cagcgcctcg
      721 tcccaggcgc tcatctgggc gaattcctcg cgataggcgc cggcgacgat attgaggccc
      781 gcgcgaccgc cgcttatgtg atccagggtc gcgatcatct tggcggccac ggcggggttc
      841 tgcagcagcg gatgcagcgt ggcccagatc ttcacccgct tggtggcctc ggccaggccc
      901 gccatcagcg tcaccgactc cagcaccgta ccccaatggt cggtcgtccc gccaaaaccc
      961 cggaacttgg ccatcgacat gatgaagtcc aggccgatct cgtcggccag cacggcggcg
     1021 gcgcggttct gcggatacag gccatcgatg cgcggcgtcg tcccggagac gatccagccg
     1081 ccattggcca ccggcaggaa gacgccgaaa tccttgtgat tggagctcat ggcttttcgt
     1141 agcacgcgac tagcgcgcgc aaaccgtcga tcttcagact gccctcactc ggcgacgtcg
     1201 atcatgcccg caggcggcgt cggcggcctc atgaacagcg cggcgacgaa cactaccgcg
     1261 atcaccgccg cgaacagctg gaaggtcggg gtgaaactgc ctagccgatc gcgcatggcc
     1321 ccggcgatga acggtcccgc cgccgagacc gcgccgacca ggcaggtcag ggaaaacagc
     1381 tccagattgc tgcgccggcc ataatagttc agcagcagca cggtgaccgc cagcaccgtc
     1441 aggccaaagc cgacgcccgt gcccacggcg tagagcagca tcatgggcag ggtggtcgcc
     1501 gcgcctagcg ccaccagccc cgccaccagc atggcctggg cgccgaccag cagccatttg
     1561 ggatcgatcc ggtcgccgat gaagccgccg cccagccggg ccagggtctg catcagagac
     1621 tcaaggctca gcatcttggc cccgatggcg gcggccgctg cggcggccat gccggtggcg
     1681 acgccgatct cggcgatgtg gctgaccgag acgctggcca ccgtcacgcc ggtcagcaga
     1741 tgggtgaaat aggccgcaac cagcacatag aactgcggcg ttcgcagcgc cgcccgcacc
     1801 gtccactcgc gcgcggtgcg atagacgcgg gcgtcggccg gcgccgcacg ggccgcctgc
     1861 tcgacctcga catcgagctc gtgagcggcc tgcgccagcc acttgctccc gccgaccagg
     1921 gccgcgcaga tcacgcccac cacgcaggcc agcacggcct ggaccatcca gtagattcgc
     1981 cagttgtcgt cggtcgcgcc catgatcatc agggccatcc aggggccggc caccccgccc
     2041 agcgagccga aggtgaaata gatcccgaac ggcagggccc gcttgcgata cagggccgac
     2101 agcacatggg tgccggggat cagggccgcc atctgaaagc cgatcccgca cagggcggcg
     2161 ccgacatagt agcgggcgat gctttccgat ccggcgaagc ccagcagccc cgcgcccagc
     2221 accgccgagc ctagcagcag ggtgccgcgc acgccgatcc ggcggatcag gatggccggc
     2281 agccaggacg aggcgccggt gaagaccccc agcacggaaa acccgagaaa cgccttctcg
     2341 aaactccagc ccagctcgcc gatcatcgcc ggcagcacca cccccagcga gccataggtc
     2401 gaggcggtga tcaggaacag cagcaggctg aagccggcca gcagcgagat gcgacgcagg
     2461 tcggtggatg ccaagatcag aacgtccgct tgagggtcac gcgccattcg cggcccttgc
     2521 cgggcagcgc gcccagattg gcataggtat cggcgtcggg ggtgaaatgg aatttgtcga
     2581 acagattatc gacattcagc tcagccgtat aaggcccgcg cgtcacatgg gccgagccgt
     2641 tgaccaccga ataggcggga tagcggacgg cgttctggac cgtgcccgcc gtgcgactca
     2701 catggctgac gcccagcgtc gcgccggcct gcccccagtc gtaggccttg ctggtatagg
     2761 cgccgtacag gctgaccacc gacttgggga tcagggtgta ggcatagtct ccgccccggc
     2821 ccggcaggct ggcaaagctc cacaccacat aggagccgcc ataggcgttg gcgccgggaa
     2881 cgcccgccgt ataggccggg atatactgga acgaggcgtc cggtcccttg atcgtggtgc
     2941 gctggctgtt gccagcgaag gtgaagctga gttgctccga agccagccag cggacctcca
     3001 gctcgacgcc cttg
//
LOCUS       DAOMEO010000301         3013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3623_length_3013_cov_3.199121, whole genome shotgun sequence.
ACCESSION   DAOMEO010000301 DAOMEO010000000
VERSION     DAOMEO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_3013_cov_3.199121"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..542
                     /locus_tag="PLH31_07875"
     CDS             333..542
                     /locus_tag="PLH31_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89168.1"
                     /translation="MVFFCYIYSLGSEVPHMEALSCSSLGEAQARCRRMLDEHGAAVR
                     AELFDDDQRVAIISRKDAYERRLQA"
     gene            complement(637..2544)
                     /locus_tag="PLH31_07880"
     CDS             complement(637..2544)
                     /locus_tag="PLH31_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR89169.1"
                     /translation="MSFGDLKIPHKLMSVFAVMLATIMLMGGALYANKLSLEASVART
                     ERAYEMLRQADTAAFRLTRQENSLRGFLLSGDTYYVERLEEGHKPKFLAALEKLKVLA
                     KGDEASLKRVATVEAGYAEFRRLAIEPGLSLGADPMTRPQAVELVSNDGVADKAVAPV
                     EESIEAIVADSEAVLAREAAAQKKASVASTITLAAGILITVGIAIAGGLLLSGGIATP
                     VAAMTAAMRRLASGDNTVEVPASGRKDEIGQMAAAVTHFKEAAIAKERLESESASQRS
                     LTERERAEREADKAADAEADATTFGALAEALDRLASGDLTHRISVDFAPKAQQLKTDF
                     NAAAARLQDAMKSISVTTEGVSAGSDEIARASDDLSRRTEQQAASLEETAAALDEITA
                     TVRKTASGAKEASQVVANARSDAQRSGEIVSQAVSAMTEIEGSSQKVGNIIGVIDEIA
                     FQTNLLALNAGVEAARAGEAGRGFAVVAQEVRALAQRSADAAKEIKVLISTSTQQVGT
                     GVSLVGQTGEALRRIVEQVASIDTLVNEIAASANEQSTGLHEVNQAVNQMDQVVQQNA
                     AMVEQATAATHALKGEASELASLVGRFKVSQAAQDAPRAAPQHRARPQTAVRPGGGSA
                     AVAVKEEWEEF"
     gene            complement(2717..>3013)
                     /locus_tag="PLH31_07885"
     CDS             complement(2717..>3013)
                     /locus_tag="PLH31_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQR89170.1"
                     /translation="QRQFSERAAINAPIQGAAADVMRRAMIRMPGALVEAGLSARVLL
                     QVHDELVFEAPEAEAEATCAVARRVMEGAAEPAVAIAVPLTVEARAALNWDEAH"
BASE COUNT          425 a         1002 c         1019 g          567 t
ORIGIN      
        1 agtcgctcgg acttggcgcg gcgctggcca ccttatgttg tcgccggcgg gcccagcttc
       61 ctattccggc gatcctggtg agcggatccg gcttgccgac gccccatgtt ggcccggcgc
      121 tctaaggcct tggctctgcg ccttcatggc cgccgttggt tcgcccccca aaatgggctt
      181 ggcggtctgg gcctttttca gtctcgggcc ctggctgccg gcttctgaca ccttcaccgc
      241 gccgctatgt cggcaacccg gcggttctcg gctgcggtag cggttatggt gccgctcggt
      301 tctccttaaa aacgagcgcc agtcggggtc gaatggtctt tttctgctac atctattcgc
      361 tcggttcaga ggtgccccac atggaggctc tgagttgctc gtcgctgggc gaggcccagg
      421 cgcgctgccg gcggatgctg gacgagcacg gcgccgccgt gcgggccgag ctgtttgacg
      481 acgaccaacg ggtggcgatc atttcccgga aagacgccta cgagcgtcgc ctgcaggcct
      541 agcgccttcc ggcaaggcgc gcgccgggcg atcacccgga ccgcgcgcct gctttttccg
      601 tctcacagct ctttgccgct ggcgccgctg gtcgggctag aactcttccc actcttcctt
      661 gaccgccacg gcggcagaac caccgccggg acgaacggcg gtttgcggcc gagcgcgatg
      721 ttgcggcgcg gcgcgcggcg catcctgggc ggcctgcgag accttgaagc gaccaacgag
      781 gctggccagt tcactggcct cacccttcag ggcgtgggtc gcggcggtgg cttgctcgac
      841 catggcggcg ttctgctgaa cgacctggtc catctggttc acggcctggt tgacctcgtg
      901 cagcccggtg gactgctcgt tggccgaagc ggcgatctca ttgaccaggg tgtcgatcga
      961 agcgacctgc tcaacgatgc gacgcagggc ctcgcccgtt tggccgacca ggctgacgcc
     1021 cgtgccgacc tgctgggtcg atgtcgagat cagcaccttg atctccttgg cggcgtcggc
     1081 agagcgctgg gccagggcgc ggacttcctg ggcgacgacg gcgaagcctc gaccggcttc
     1141 accagcccgc gcagcctcga cgccagcgtt gagggccagc aggttggtct ggaaggcgat
     1201 ctcgtcgatg acgccgatga tattgccgac cttctgcgag gagccctcga tctcggtcat
     1261 ggccgagacg gcctggctga cgatctcgcc cgaacgctgg gcgtcgctgc gggcgttggc
     1321 gaccacttgc gaggcctcct tggcgcccga ggccgtcttg cggaccgtgg cggtgatttc
     1381 gtcgagggcg gcggcggttt cttccaggct ggcggcctgc tgttcggtgc ggcgcgacag
     1441 gtcgtcagag gccctggcga tttcgtcgga gccggcgctg acgccctcgg tcgtgaccga
     1501 gatgctcttc atcgcatcct gtagacgggc ggcggcggcg ttgaagtcgg ttttcagctg
     1561 ctgggccttg ggcgcgaagt ccactgagat gcggtgggtc agatcgccac tggccagccg
     1621 gtcgagggcc tcggccagag ccccgaacgt cgtggcgtcc gcctcggcgt ccgcggcctt
     1681 gtcggcctcg cgttcagcgc gctcgcgctc ggtcagcgag cgctgactgg cggactcgct
     1741 ctcgagacgc tccttggcga tggcggcttc cttgaagtgg gtgacggcgg cggccatctg
     1801 gccgatctcg tccttgcggc ccgaggccgg cacctcgacg gtgttgtcgc ccgaggccag
     1861 acggcgcatc gcggcagtca tggctgcgac cggcgtggcg atgccgcccg acagcaacag
     1921 accaccggcg atcgcgatac ccacggtgat caggataccg gcggccagtg tgatggtcga
     1981 ggcgacggac gccttcttct gggcggcggc ttctcttgcc agtacggcct cgctgtcggc
     2041 aacgatggcc tcgatgctct cctcgactgg cgcaacagcc ttgtcagcga ccccgtcatt
     2101 gctgaccagt tcgacggcct gaggacgggt catcgggtcg gcgcccaggc tgagcccggg
     2161 ctcaatggcc aggcggcgga attcggcata accagcttcg acggtcgcga cgcgcttgag
     2221 gctggcctca tcgcccttgg ccaggacctt cagcttctcg agcgcggcga ggaacttagg
     2281 cttgtgtccc tcttccagac gctcgacata ataggtgtcg cccgagagca ggaagccgcg
     2341 cagcgagttc tcctgacggg tcagccggaa ggccgcagtg tcggcttggc gcagcatttc
     2401 ataggcgcgc tcggtacggg cgaccgaagc ctccaagctc agcttattgg cgtacagcgc
     2461 tccccccatg agcatgatcg tggcaagcat gacggcgaac accgacatca gcttgtgggg
     2521 aattttcaga tcgccgaacg acacagccgc cctccagggt ttagtgatgg cgctccgaaa
     2581 ccctagataa cacagatatt tagatccgcg tccaaggcta tgcgccgccc ctagctgtgg
     2641 cgtgcgatgg gtttccgccg tgttaaccgt gacgcggcgg aacgtcgcat cgccgacagt
     2701 tctgccggct ttttgatcaa tgggcctcat cccagttgag ggcggcgcga gcctccaccg
     2761 tcagaggcac ggcaatggcc acggccggtt cggcggcgcc ttccatcacc cggcgcgcca
     2821 cggcgcaggt cgcttcggcc tcggcttcgg gggcctcgaa gaccagttcg tcgtgaacct
     2881 gcagcagcac cctggccgac agcccggcct cgaccagcgc gcccggcatg cggatcatcg
     2941 cgcggcgcat gacatcggct gccgcgccct ggatcggcgc gttgatcgcc gcccgctcgg
     3001 agaactgtcg ctg
//
LOCUS       DAOMEO010000302         3009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3629_length_3009_cov_2.614760, whole genome shotgun sequence.
ACCESSION   DAOMEO010000302 DAOMEO010000000
VERSION     DAOMEO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_3009_cov_2.614760"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>224
                     /locus_tag="PLH31_07890"
     CDS             <1..>224
                     /locus_tag="PLH31_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89171.1"
                     /translation="AAELEAVRAARAAAREVVRQEQLAKQEAILAAKRAERKERKTDA
                     AAEQRMRKEEKAAQREQLRSLGRTSKSARA"
     assembly_gap    225..234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            337..>595
                     /locus_tag="PLH31_07895"
     CDS             337..>595
                     /locus_tag="PLH31_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQR89172.1"
                     /translation="MDVFQPDLLATMFAVAALAGAFDAIAGGGGLLTLPALLLAGLDP
                     VAALATNKLQGSFGSVSSTLAFARRGLIDWKTALPVALAAGL"
     assembly_gap    596..605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <606..1126
                     /locus_tag="PLH31_07900"
     CDS             <606..1126
                     /locus_tag="PLH31_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQR89173.1"
                     /translation="CASLLSPTFLKALVPILLIVIALYFGLSKALKTEDGHGRLALPV
                     FAGFVAPLIGFYDGIFGPGAGAFYMVAIVTLLGYGALKATAHTKLANAASNLGSLALF
                     ILKGAVIWPVGLAMAAGAFLGAQVGSRLAMRFGAGLIRPLLVTMSCAMAVKLLADPQN
                     PLRQALSHLIGS"
     gene            complement(1131..>3009)
                     /locus_tag="PLH31_07905"
     CDS             complement(1131..>3009)
                     /locus_tag="PLH31_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQR89174.1"
                     /translation="ALAAALGDDLDAALDSRAPSYWGGAETPAPTWPEGAQALAPLVK
                     APPELAARLAFTAVVERAEGERIQKALGPGMRLVSREGDLWRWDGFVARADAPKPAAV
                     RLEQRTRLAEVEAEIDLTTPRAEATAAALKLASDRLRAAEDSLRDRRRGPPEAERLLT
                     GARDAVAQLERAAALRAARAQSLDETIARFQAEHAEAETALTAVRAERAGATGAGDLA
                     PQLAEARQAAAAAREAASTARAALDVETRERAGRQRRLESLERDHGDWARRAEAAGKR
                     TAALDADRIKAAVALEAAREAPATLKEKLLVLLDEFGAAEARRAKASDALEEGESARL
                     AADRAARAADQAAGEARERRAALVAHLDGARGRFAEIAGNIRESVRMEPEELGRHVAG
                     EAVAIPKDAVGVEAHLFALERERDAIGPVNLRAEEEAQEYAGRLETMRTERADLSGAV
                     GKLRAGIEELNAEGRERLLAAFEVINGHFQTLFQALFGGGQAELRLIESDDPLEAGLE
                     IYACPPGKRMASMSLMSGGEQALTASALIFGVFLANPAPICVLDEVDAPLDDANVDRY
                     CNMLDEMRRRTQTRFIAITHNPVTMSRMDRLFGVTMGERGVSQLVSVDLSTAEQLVAA
                     Q"
BASE COUNT          392 a         1059 c         1064 g          474 t
ORIGIN      
        1 cgccgccgag ctggaagccg tgcgcgccgc ccgcgccgcc gcccgcgagg tcgtccgcca
       61 ggagcagttg gccaagcagg aagcgatcct ggccgccaag cgcgccgagc gtaaggaacg
      121 caagaccgac gccgccgccg aacagcggat gcggaaggaa gagaaggccg cccagcgcga
      181 gcagcttcgc tccctgggcc gcaccagcaa gagcgcccgc gctcnnnnnn nnnnaggcct
      241 ttgcgctttc ccatctctgc tcgagcccct tcttcctggg cctagactcg accgcatggt
      301 cctggctctc gccagtccat gcgggagggc cggctcttgg acgtattcca acctgacttg
      361 ctggcgacga tgttcgccgt ggctgcgctg gccggagcct tcgacgccat agcgggcggg
      421 ggagggcttt tgacactccc ggctcttctg ctggcggggc ttgaccccgt tgcggcgctg
      481 gccaccaaca agctgcaggg ctctttcggc tcggtgtcct cgaccctggc cttcgcccgg
      541 cgcggcctga tcgactggaa gaccgccctg ccggtcgccc tcgccgccgg cctcgnnnnn
      601 nnnnntgtgc gccagcctgc tgtcgccgac ctttctcaag gcccttgtgc cgatcctgct
      661 gatcgtcatc gccctctatt tcggcctgtc caaggccctg aagaccgagg acggccacgg
      721 ccggctggcc ctgccggtgt tcgccgggtt cgtcgcgccg ctgatcggct tctatgacgg
      781 catttttggc cccggcgcgg gggccttcta catggtcgcc atcgtcaccc tgctgggcta
      841 cggcgcgctg aaggccacgg cccataccaa gctggccaac gccgccagca atctgggcag
      901 cctggctctg ttcattctca agggcgcggt gatctggccg gtgggcctgg cgatggccgc
      961 cggcgccttc ctcggcgcgc aggtcggctc aaggctcgcc atgcggttcg gagccgggct
     1021 gatccggccg ctactggtga cgatgtcgtg cgccatggcg gtgaagctgc tggccgatcc
     1081 ccagaacccg ctgcgccagg cgctgtcgca tctgatcggc agctgaaagc ctactgggcc
     1141 gccacgagct gctcggcggt cgacagatcc acgctgacca gctggctgac gccgcgctcg
     1201 cccatggtga cgccgaacag gcgatccatt cgactcatcg tcaccggatt gtgggtgatg
     1261 gcgatgaagc gggtctgggt gcggcgacgc atctcgtcga gcatgttgca gtagcgatcg
     1321 acattggcgt catccagcgg cgcatcgact tcgtccagaa cgcagatcgg ggcggggttg
     1381 gcgaggaaga cgccgaagat cagggcgctg gcggtcaggg cctgctcgcc gccgctcatc
     1441 aggctcatcg acgccatccg cttgccgggc ggacaggcat agatctcgag cccggcttca
     1501 aggggatcgt cgctctcgat cagccgcagt tcggcctgac cgccgccaaa cagggcctgg
     1561 aagagggtct ggaagtggcc gttgatcacc tcgaaggcgg ccagcagacg ctcgcgcccc
     1621 tcggcgttca actcctcgat gccggcccgc agcttgccga cggcgccgga caggtcggcc
     1681 cgttcggtgc gcatggtctc caggcggccg gcatattcct gtgcctcttc ctcggcccgc
     1741 agattgaccg ggccgatggc gtcgcgctct ctctcgagcg cgaacaggtg tgcttcgacg
     1801 cccacggcgt ccttggggat cgccacggct tcacccgcga catgacggcc cagttcctcg
     1861 ggttccatgc ggaccgattc gcggatgttg ccggcaatct cggcgaagcg gccgcgagcg
     1921 ccgtccagat gagcgaccag ggcggcgcgg cgctcgcggg cctcgccagc ggcctgatcg
     1981 gcggctcggg cggcgcggtc ggcggccagg cgagcggatt cgccttcctc cagcgcgtcg
     2041 ctggccttgg cccggcgagc ttcggccgcg ccgaactcgt cgagcaggac gagcagcttt
     2101 tccttcagcg tcgccggcgc ctcgcgggcg gcttccaagg cgacggcggc cttgatccgg
     2161 tcggcgtcca gggctgcggt acgcttgccc gcagcctcgg cgcggcgtgc ccaatcgccg
     2221 tgatcgcgct ccaggctttc gaggcgacgc tggcggccgg cccgctcacg ggtctcgaca
     2281 tccagagccg cgcgcgcggt tgaggcggcc tcccgtgcgg cggcggcggc ctgacgggct
     2341 tcagcgagtt gcggcgccag atcgccagcg ccggtcgcgc cggcgcgctc ggcccgcacg
     2401 gcggtcaggg ctgtctcggc ctcggcatgc tcggcctgaa agcgggcgat ggtctcgtcc
     2461 agcgactgag cgcgggcggc gcgtagcgcg gccgcgcgtt ccaactgggc gacggcgtca
     2521 cgggcgccgg tcagcagacg ctcggcttca ggcgggccgc ggcgcctgtc acgcagcgaa
     2581 tcctcggcgg cgcgcaggcg atccgaggcg agtttcaggg cggcggcggt ggcttcggcg
     2641 cggggggtcg tcaggtcgat ctccgcctcg acctcggcca gacgggtgcg ctgctccagc
     2701 cgcacggcgg caggcttggg cgcatcggcg cgcgcaacga aaccgtccca gcgccacagg
     2761 tcgccctctc gcgagaccag gcgcatgccg gggccaagcg ccttctggat ccgttcaccc
     2821 tcggcgcgct cgaccacggc ggtgaaggcc aggcgggcag ccaattccgg tggggccttg
     2881 accagcggag ccagggcctg ggcgccttcg ggccaggtcg gggcgggcgt ttcagcgcca
     2941 ccccagtagg acggcgcccg ggagtccagg gcggcgtcga gatcgtcgcc aagggcggcg
     3001 gccagggcc
//
LOCUS       DAOMEO010000303         3004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3633_length_3004_cov_2.504239, whole genome shotgun sequence.
ACCESSION   DAOMEO010000303 DAOMEO010000000
VERSION     DAOMEO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_3004_cov_2.504239"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLH31_07910"
     CDS             <1..474
                     /locus_tag="PLH31_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HQR89175.1"
                     /translation="AFFLMKPKPEAHGEKGAEEKDKKKEKKKDEKKKEGEGAAAGAEG
                     AAGTPVIKEGPDGIVFYTLPDIVVNMQTADGKSTFLKLKLTFELPDAETAEVLTPNLP
                     RLQDMFQTFLRELRPEDLNGSQGTFQLRVELLRRVNLVAAPSKVNAVLIEEMLIN"
     assembly_gap    532..541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            683..1711
                     /gene="fliM"
                     /locus_tag="PLH31_07915"
     CDS             683..1711
                     /gene="fliM"
                     /locus_tag="PLH31_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HQR89176.1"
                     /translation="MGGGGWDDGGGDMAGSERILNQDEIDSLLGFDLSGDGNDDRTGI
                     RAIINSALVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDNISSIRFGDYLNS
                     IPLPAILAVFRAEELDNYGLLTVDSNLIYSIVDVLLGGRRGTAAMRIEGRPYTTIERV
                     LVQRMIEVVLHDLKAAFEPLHPVSFSLDRLETNPRFAAIARPANAAILVKLRIDMEDR
                     GGRIELLLPYATLEPIRKMLLQQFMGEKFGRDNIWEGHLATELWTTQMEVRAVLDEQQ
                     LPLSRVLDLQVGDTMMLNATPDSLVELRAGSIPLTRGRMGRRNHSIAIRAEAPLTPAA
                     KKAVQKLK"
     gene            1708..2262
                     /locus_tag="PLH31_07920"
     CDS             1708..2262
                     /locus_tag="PLH31_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HQR89177.1"
                     /translation="MSLIAVAMNALLAVLLIVALGFGWRLERRLKALRDSHAGFAKAV
                     ADLDQAAARAEQGLADLRAATDEASEALADRIERATVLAAKLDERLNRPMVTPPPAAA
                     PTPARREAPELKADRRLKAEDFERLLDREAQASRPIEPPARAAPISRPSVAKETPRSR
                     ARVDDDLFEGPVDPPRPGPSFRAR"
     gene            2262..>3004
                     /locus_tag="PLH31_07925"
     CDS             2262..>3004
                     /locus_tag="PLH31_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HQR89178.1"
                     /translation="MKNIPRILPLVGVAVGGVLAVNALAGAKSLPDLLGGAKAFAEGV
                     AKPDAKSAGEEAKSAESAGQNAAASAASSLPPRVCAPSATDLAREAGLSPAELRILQS
                     LGARRGQLDQREQDIDVQLQLLAAAEAKLDAKVKALNGLKGDIQGLLGQADTQKSAEV
                     DRMVTVFSSMKPKDAAARMTILDDSVRLPIAAKMKERTLSLILANMLPTEAKVLTERL
                     ANRFANDAINKGKAAIADSGAAQTDAAKRA"
BASE COUNT          551 a         1028 c          969 g          446 t
ORIGIN      
        1 gccttcttcc tgatgaagcc caagcccgaa gcccatggcg aaaaaggcgc tgaggaaaag
       61 gacaagaaga aggaaaagaa gaaggacgag aagaaaaagg aaggcgaggg cgccgccgct
      121 ggcgccgagg gcgcggccgg aacgccggtg atcaaggaag gtcctgacgg gatcgttttc
      181 tacaccctgc ccgacatcgt cgtgaacatg cagacggccg atggcaagtc gaccttcctg
      241 aaactcaagc tgaccttcga gctgcccgac gcggaaacgg ccgaggttct gacaccgaat
      301 ctgcccaggt tgcaggacat gttccagacc ttcctgcgcg aactgcgccc cgaagacctg
      361 aacggcagcc agggcacctt ccagcttcgc gtcgagctgc tgcgccgcgt caatctcgtc
      421 gccgcaccgt caaaagtgaa cgctgtcctg atcgaggaga tgctgatcaa ctagcccctt
      481 cgggagctgg ttgggcaaac caacatggcg gacgaactag acgatcaggc cnnnnnnnnn
      541 nccaatgacc aggcaggcaa attgggcgca ttccaccccg ttatgacata ccccgggggc
      601 ttcgcagaac cctccgggcg ctttcgagca gccggcggag gggagcgggg ctgcgggcga
      661 atttggcgat ttcagcggcg gcatgggcgg cggcggctgg gacgacggcg gcggcgacat
      721 ggcgggatcc gagcggatcc tgaaccagga cgagatcgac agcctgctgg gcttcgatct
      781 gtcgggcgac ggcaatgacg accgcaccgg catccgggcg atcatcaact cggccctggt
      841 cagctatgag cgtctgccaa tgctcgaaat cgtcttcgac cgcctcgtgc ggttgatgac
      901 gacgtcgctg cgcaacttca cgtccgacaa cgtcgaggtc agcctcgaca acatcagctc
      961 gatccgattc ggcgactatc tgaactcgat ccccctgccg gccatcctgg cggtgttccg
     1021 ggccgaggag ctggacaatt acggcctgct gaccgtcgat tccaacctga tctactcgat
     1081 cgtcgacgtg ctgctgggcg gccggcgcgg caccgccgcg atgcggatcg agggccggcc
     1141 ctacaccacc atcgagcgcg tgctggtcca gcggatgatc gaggtggtcc tgcacgacct
     1201 gaaggccgcc ttcgagcccc tgcacccggt cagcttctcg ctggatcgtc tggagaccaa
     1261 tccgcgcttc gccgccatcg cgcgtccggc caacgccgcc attctggtca agctgcgcat
     1321 cgacatggaa gatcgtggcg gccgcatcga gctgctgctg ccctatgcga cgctggagcc
     1381 catccgcaag atgctgctgc agcagttcat gggtgagaag ttcggtcgcg acaatatctg
     1441 ggaaggccac ctggccaccg aactctggac cacccagatg gaggttcgcg ccgtgcttga
     1501 cgagcagcaa ctgcccctgt cccgcgtgct ggacctgcag gtcggcgaca ccatgatgct
     1561 caacgccacg ccggacagcc tggttgaact gcgggccggc tcaatcccgc tgacgcgggg
     1621 ccgcatgggg cggcgtaacc actccatcgc catccgtgcc gaggcgccgc tgacgccggc
     1681 ggccaagaaa gcggtccaga agctgaaatg agcctgatcg ccgtcgccat gaacgctctg
     1741 ctcgcggtcc tgctgatcgt ggccctgggc tttggctggc gcctggagcg ccggcttaag
     1801 gccctgcgcg acagccatgc cggcttcgcc aaggcggtcg ctgatctcga ccaggccgct
     1861 gcgcgcgccg agcagggcct ggctgatctg cgcgccgcca ccgacgaggc gtccgaggcc
     1921 ctggcggacc gcatcgagcg cgccaccgtc ctggccgcca agctggacga gcgcctgaac
     1981 cgtccgatgg tcacgccgcc ccccgccgcc gccccaacgc cagcacgccg agaggcgccg
     2041 gagctcaagg ccgatcgtcg cctgaaggcc gaggacttcg agcgtctgct ggatcgcgag
     2101 gcccaggcca gccggccgat cgagcctcca gcgcgcgccg cgcccatttc gcgacccagt
     2161 gtcgcaaagg aaaccccgcg ttcacgagcg cgggttgatg atgacctctt cgagggcccg
     2221 gtcgatccgc cgcgtcccgg acccagtttc agagcccggt gatgaaaaac attcctcgta
     2281 tcctcccgtt ggtcggcgtc gccgtcggcg gcgtcctggc cgtaaacgcc ctggccggcg
     2341 ccaagtcgct gcccgacctg ctgggcggcg ccaaggcttt cgccgagggc gtggccaagc
     2401 ctgacgccaa gtcggcgggc gaagaggcca agtcggcgga gtccgccggt cagaacgcgg
     2461 ccgcctctgc ggcatcgtcc cttccgcccc gcgtctgcgc gccttccgcc acggatctgg
     2521 cccgtgaagc cggcctatcg cccgccgaac tgcgcatcct gcaaagcctg ggcgcccgtc
     2581 gcggccagct ggaccagcgc gagcaggaca tcgacgtcca gctgcagctg ctggctgccg
     2641 cggaagccaa gctggacgcc aaggtcaagg cgctgaacgg cctcaagggc gacatccagg
     2701 gcctgcttgg ccaggccgac acccagaagt cggccgaggt cgaccgcatg gtcaccgtgt
     2761 tctcgagcat gaagcccaag gacgccgccg cgcgcatgac gatcctggac gacagcgtgc
     2821 ggctgccgat cgccgccaag atgaaggagc gcacgctcag cctgatcctg gcgaacatgc
     2881 tgccgactga agccaaggtc ctgaccgagc gtctggccaa ccgcttcgcc aatgacgcca
     2941 tcaacaaggg caaggccgcc atcgctgaca gcggcgcggc tcagaccgac gcagccaagc
     3001 gggc
//
LOCUS       DAOMEO010000304         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3637_length_2999_cov_2.798573, whole genome shotgun sequence.
ACCESSION   DAOMEO010000304 DAOMEO010000000
VERSION     DAOMEO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3637_length_2999_cov_2.798573"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLH31_07930"
     CDS             complement(<1..326)
                     /locus_tag="PLH31_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HQR89179.1"
                     /translation="MPSSAFAPLTGALTHFEAQALTLDDPRPHPHEDALIQLGHAVLT
                     ETLDVFGETALEDFQAIICETLIGAFHSAAQRIERDADRARDELNRLSRDFDGSEIAD
                     TEMQDAT"
     gene            complement(564..1172)
                     /locus_tag="PLH31_07935"
     CDS             complement(564..1172)
                     /locus_tag="PLH31_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89180.1"
                     /translation="MGWLYMRSLEGHATPKAYLDAQFTHDRDTGRSRVLASSVVGRRT
                     YYAAVEWISAGTEAREVWGLVCLVRHNPRDREGLVFGYKDMSESMGPCESDCPARILD
                     LLTATDNTYALAWRERCGSGLVLRAGRAAKPTPKPGDVVVLAEPLLFRDGRTFSRFEA
                     VSLPGRKRGGLVYRSELGGLYRIPKLKDRVYLIERKAGRSHA"
     gene            complement(1209..2108)
                     /locus_tag="PLH31_07940"
     CDS             complement(1209..2108)
                     /locus_tag="PLH31_07940"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HQR89181.1"
                     /translation="MSNPLNALINDIIARIEAGVPPWRQAWKASADPKAPLRATGEPF
                     SGSNAWLLAFAGALRGYRSPYWFTFRQALSINAPVAKGEKGSPAILYKTRVVGDPADG
                     ADVGPADDTGDPRILRYLKTYSVFNAEQLTDCPQQYLNVVEPDPAARAAARNAVLDAI
                     PARVEFGRGTPCYVPALDLIRLPLPEAFDTPDDFLATKAHEQLHWSGAAHRLDRTFGK
                     RFGDEAYAFEELVAEIGAAALGLRIGLAPQMLDSHAAHLGHWAKILRHRPSALLEASG
                     HAQRAVDYLLAFSAQAAVADLAA"
     gene            complement(2242..>2999)
                     /locus_tag="PLH31_07945"
     CDS             complement(2242..>2999)
                     /locus_tag="PLH31_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HQR89182.1"
                     /translation="QVRTARALMSAETALAETDPEARVFAVNSKSGRAALVVRGLTTT
                     NDDDRLVPAVRLIRPEKRSVAPLKAYEESAWEGTDEAAWRAAWDAELAAADPWVTREL
                     VLVAGLLLPIWSSLPDKRTSVRRLKAPDGRRWLGRLLDPAQVTPLKIALGLTDTASAV
                     GDGEAATRLVLSEGASLALSGGLWLRRAKVMDRWRLEIVGAGAQRTSFVALGCFVEII
                     AYTPRVFVPTDRPDVLSEILDRWPAQTILPAAA"
BASE COUNT          494 a         1016 c         1030 g          459 t
ORIGIN      
        1 gtggcgtcct gcatctcggt atcagcgatc tccgagccgt cgaagtcccg gctcagccga
       61 ttgagctcgt ccctggcccg atccgcgtct cgttcgatgc gttgggcggc cgagtgaaac
      121 gccccgatga gcgtctcgca gatgatggcc tggaaatcct cgagtgcggt ctcgccaaag
      181 acatcgaggg tctcggtcag caccgcatga cccaactgga tcaaagcatc ctcgtgaggg
      241 tggggccggg ggtcatccag ggtcagggcc tgggcttcga agtgggtgag cgcgccggtc
      301 agcggcgcga aggcggagga gggcatgagg gtgatccttg tcggagtggg gttctgagcc
      361 cgtcggctcg acgaccgccc agcgccgacg gctggggacg cccacaaggg ctttgagcgg
      421 agcgtccctt gtggggggcg ccagacggtg gcgaggtctc aagagccaga ggactccagg
      481 accgcagcac cgccgacgcc aaccgtttgc cgcctcggct tcagcgtttg gcgctgccac
      541 gctccacccg tccgcccatg cgctcaggcg tgactgcgcc ccgccttccg ctcgatgagg
      601 tagacgcggt ccttgagctt ggggatgcgg taaaggccgc ccaactcgct gcgatagacg
      661 agaccgccgc gcttgcgtcc aggcaaagag accgcctcga agcgggagaa cgtgcgcccg
      721 tctcgaaaca acagcggctc ggccagcacc acgacatcac caggcttggg tgtcggcttg
      781 gcggcgcgcc cagcccgaag gaccagaccc gacccgcacc gctcgcgcca agccagggcg
      841 taggtgttgt cggtcgccgt cagcaggtcg agaatgcgcg cagggcagtc ggactcgcac
      901 gggcccatgg attcggacat gtctttgtag ccgaacacta agccctcccg gtcgcgcggg
      961 ttatggcgca ccaggcacac caggccccag acctcccgcg cctctgtacc ggcgctgatc
     1021 cattcgacgg cggcgtagta ggtgcgccgt ccgaccacac ttgaggccag cacgcgagag
     1081 cggccggtgt cccggtcgtg ggtgaattgc gcatcgagat aggctttggg ggtcgcgtgc
     1141 ccctccagcg agcgcatgta gagccagccc atggcggcct ccttgcaaga tgatgggaaa
     1201 ggccggcttc aggcggccaa gtcggcgacc gcggcctggg cgctgaacgc cagcagatag
     1261 tcgacggccc gttgggcatg gccggacgct tcgagaaggg ccgagggtcg atgccgcagg
     1321 attttcgccc agtggccaag atgggccgca tggctgtcca gcatctgcgg tgcgagcccg
     1381 atgcgcagac cgagcgccgc cgcgccgatc tcggcgacca gttcttcgaa ggcgtaggcc
     1441 tcgtcgccga agcgtttgcc gaaggtgcga tccaaccgat gagccgcgcc cgaccagtgc
     1501 agctgctcgt gcgccttggt ggccaggaag tcgtcgggcg tgtcgaaggc ttccggaagg
     1561 ggaagccgga tcagatcgag cgccggcaca tagcagggcg tcccgcggcc aaattccacc
     1621 cgagccggaa tcgcatccag cacggcgttg cgcgctgccg cgcgggcggc cggatcgggt
     1681 tcgacgacgt tgagatattg ctgcggacag tctgtcagct gctcggcgtt gaacacgcta
     1741 taggtcttca agtagcgcag gatacgagga tcgccggtgt cgtcggccgg tcccacgtcg
     1801 gctccatcgg cgggatcacc caccacccgg gtcttgtaga ggatcgcggg cgagcccttc
     1861 tcacccttgg ccacaggggc attgatcgaa agcgcttggc ggaaggtgaa ccagtagggc
     1921 gagcgatagc cgcgcaacgc gcccgcgaag gcgagaagcc aggcgttgct gccgctgaag
     1981 ggctcgccgg tcgcgcgtag tggcgccttg ggatcggcgc tggccttcca ggcctggcgc
     2041 catggcggca cgccagcctc gatgcgggcg atgatgtcat tgatcagggc gttgaggggg
     2101 ttggacatgg gaacctcggg ctcgcccccg ggccaatccc ggagcgcttg cccgctccgt
     2161 cccttcctcc agcccttttc cggccaggcc gccggtatgg aaagagccgc cggcggatca
     2221 ccggcggctc cctcatccca gctaggccgc ggccggcagg atcgtctggg cgggccagcg
     2281 atctaggatc tcgctcagca cgtcgggtcg atcggtcggc acaaagacgc gcggcgtgta
     2341 ggcgatgatc tcaacgaagc aaccaagcgc cacgaagctg gtgcgctgag cccccgcccc
     2401 gacgatctcc agccgccagc ggtccatcac cttggcccgg cgcagccaca gcccgccgct
     2461 caaggccaag gaggctccct ccgaaaggac cagccgggtg gccgcctcgc catcacccac
     2521 ggcgctggcg gtgtcagtca gccccagcgc tatctttagg ggcgtgacct gggccgggtc
     2581 cagaagacgg ccaagccaac ggcgcccgtc aggcgccttc agccgtcgca ccgaggtccg
     2641 cttgtcaggc aggctgctcc agatcggcaa cagcaacccc gccaccaaca cgagctcgcg
     2701 ggtgacccac gggtccgccg ccgccaattc ggcgtcccag gccgcgcgcc aggcggcctc
     2761 gtccgtcccc tcccaggccg attcctcata ggccttcagc ggcgcgacgc tgcgcttctc
     2821 tggccggatc agacggaccg ccgggacgag ccggtcgtcg tcgttggtcg tggtcaggcc
     2881 ccgaacgacc aaggccgcgc ggccggactt cgaattgacg gcgaacaccc gtgcttccgg
     2941 atcggtctcc gccaaagctg tctcggcgct catcagcgcg cgggccgtgc gcacctgga
//
LOCUS       DAOMEO010000305         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3643_length_2997_cov_2.215160, whole genome shotgun sequence.
ACCESSION   DAOMEO010000305 DAOMEO010000000
VERSION     DAOMEO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_2997_cov_2.215160"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLH31_07950"
     CDS             complement(<1..414)
                     /locus_tag="PLH31_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQR89183.1"
                     /translation="MIQQYGWSGPLAQDFEPHARAGQTPGRVVVQRRGAYLVATDEGE
                     LRAKPAGRLLHDAGEAEHPVVGDWVALTVNKAQRTATIHAILPRRTAFMRRAADSVQT
                     LQVIAANIDVAFVVTSMNADFNPRRLERYLAAAWQS"
     gene            complement(537..1538)
                     /gene="rfaD"
                     /locus_tag="PLH31_07955"
     CDS             complement(537..1538)
                     /gene="rfaD"
                     /locus_tag="PLH31_07955"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQR89184.1"
                     /translation="MTRKTVLVTGGAGFIGSNIVARLCEDPTIDVVVCDRLREAAGGK
                     WRNLSKHAIADFVAPDDLMAWLARRGGDVELVIHMGAVSSTTEPDADKIIQSNFVLPR
                     DLWDWCAEHDKRLIYASSAATYGDGSQGFDGLDDLDSLKALRPLNAYGWSKALFDIYA
                     VREASRGRKPLQWVGLKFFNVYGPNEDHKDGMKSVVAQIWPKIAAGEGVKLFKSHHPD
                     YADGGQLRDFVYVRDVADIVAWLAENPQVNGVYNLGSGQARTFRALAEATFHAIGRQP
                     DIAYVDMPEVLRGKYQYFTEAKMERLRAAGFTAPMTSLEDGVGDYVAGYLNTDDPYR"
     gene            complement(1535..2401)
                     /gene="kdsA"
                     /locus_tag="PLH31_07960"
     CDS             complement(1535..2401)
                     /gene="kdsA"
                     /locus_tag="PLH31_07960"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQR89185.1"
                     /translation="MSQPQAVAPNAVVEVKTATGAPVRIGNGEKLSIIAGPCQMESRQ
                     HALETALALKEMAARLDVGLIYKTSYDKANRTSANAQRGIGLHDSLAIFQEIREVTGL
                     PTLTDVHEISHCAPVAEAVDVIQIPAFLCRQTDLLVAAANTGRAINIKKGQFLAPWDM
                     KNVIAKVTGAGNPNVMACERGVSFGYNTLVSDMRSLPIMKEIGCPVVFDATHSVQQPG
                     GLGGSSGGQREFVPVLARAAVSIGVACVFMETHPDPDNAPSDGPNMVPMSQFEALIAN
                     LLEYDALTKRAA"
     gene            2470..>2997
                     /locus_tag="PLH31_07965"
     CDS             2470..>2997
                     /locus_tag="PLH31_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQR89186.1"
                     /translation="MRIDTAGDGPILVWLRRDLRLTDNPALDAAVSTGRSVIPLYILD
                     ETEGVRPMGAASLWWLDKSLAALGADIEQRGGRLILRRGKAAEILNQLVAETGAAGVV
                     WNRLYDKASIDRDAAIKADLRGQGLTCESFNASLLNEPWTVKNGSGEGYKVFTPYWRA
                     AREHIGEVHVSPAPAA"
BASE COUNT          511 a          948 c         1025 g          513 t
ORIGIN      
        1 gctttgccag gccgcagcca gatagcgctc aagtcgacgc ggattgaagt cggcattcat
       61 cgaggtgacg acgaaggcga cgtcaatgtt tgcggcgatg acctgcaggg tctgaacact
      121 gtcggcagca cggcgcatga aggcggtgcg gcgcggcagg atggcgtgga ttgtggccgt
      181 acgctgggcc ttgttgacgg tgagcgcgac ccagtcgcca acgaccgggt gctccgcctc
      241 cccagcatcg tgaagcaggc ggccggcagg cttggcgcga agctcgccct cgtcggtggc
      301 caccagatag gcgccacgtc gctggacgac cacgcgcccg ggggtctggc cggcgcgggc
      361 atgcggctcg aagtcctgcg ccagggggcc ggaccagcca tactgctgga tcaatggaat
      421 ggtctttcct aacggatggc cagtctagca ccccgcgggc gtcgcggctt cactccacgc
      481 agcgcgccgc acggatcgga aggcatgacg tccggcccag gcaggcgcac agcgggtcag
      541 cgatacgggt cgtctgtatt cagatagccc gccacgtagt cgcccacgcc gtcttcaagc
      601 gacgtcatcg gcgcggtgaa acctgcagcc cgcagccgtt ccatcttcgc ttcggtaaag
      661 tactggtact tgccgcgcag cacttcgggc atgtcgacat aggcgatgtc aggctggcgt
      721 ccgatggcgt ggaaagtcgc ttcggcaaga gccctgaagg tgcgcgcctg gcccgaaccc
      781 aggttgtaga cgccattgac ctgcgggttt tcggccagcc aggcgacgat gtcggccacg
      841 tcgcgcacat agacgaaatc gcgcagctgc ccgccgtctg cgtagtccgg gtggtgcgac
      901 ttgaagagct tgaccccctc gcccgccgca atcttgggcc agatctgggc gactaccgac
      961 ttcatgccgt ccttgtggtc ctcgttgggg ccatagacgt tgaagaactt cagtccgacc
     1021 cattgcaggg gcttcctgcc gcgagacgcc tcgcggacgg catagatgtc gaacagggcc
     1081 ttggaccagc cataggcgtt cagcggtcgc agggccttca gcgagtccag atcatcgagg
     1141 ccatcaaaac cctgcgaacc gtcgccatag gtcgccgccg acgaggcata gatcagccgc
     1201 ttgtcatgct cggcgcacca gtcccacagg tcccgcggca ggacgaagtt ggactggatg
     1261 atcttgtcgg catccggctc ggtggtcgac gagaccgcgc ccatgtggat caccagctcg
     1321 acgtcgccgc cgcgcctggc caaccaggcc atcaggtcgt ccggcgcaac gaagtcggca
     1381 atggcgtgct tggagaggtt gcgccacttg ccgcccgccg cctcgcgcag gcggtcgcag
     1441 accaccacgt cgatggtggg atcctcgcaa agcctggcga cgatattgga gccgatgaaa
     1501 ccggcgccgc cggtgacgag aacggtcttc cgggtcatgc ggcccgcttg gtcagggcgt
     1561 cgtattccag gagattggcg atcagggcct cgaactggct catcggcacc atgttggggc
     1621 cgtcggacgg ggcgttgtcg gggtcggggt gggtttccat gaacacgcag gccacgccga
     1681 tcgacacggc ggcgcgggcc agcaccggca cgaactcgcg ctggccgccc gaggagccgc
     1741 ccagtccgcc aggctgctgc acgctgtggg tggcgtcgaa gacgaccgga cagccgatct
     1801 ccttcatgat cggcagggag cgcatgtcgc tgaccagggt gttgtagccg aacgacacgc
     1861 cgcgctcgca ggccatgacg ttgggattgc ccgcgcccgt caccttggcg atgacgttct
     1921 tcatgtccca cggggccagg aactggccct tcttgatgtt gatcgcccgg cccgtgttgg
     1981 cggcggcgac cagcagatca gtctggcggc acaggaaggc cgggatctgg atcacgtcga
     2041 ccgcctcggc caccggggcg cagtggctga tctcatgcac gtcggtcagc gtcggcaggc
     2101 cggtgacctc gcggatctcc tgaaagatcg ccagactgtc gtgcagtccg ataccgcgct
     2161 gggcgttggc cgaggtgcgg ttggccttgt cgtaggaggt cttgtagatc aggccgacgt
     2221 ccaaacgggc ggccatctct ttcagggcga gggccgtctc cagcgcgtgc tggcggcttt
     2281 ccatctggca ggggccggcg ataatcgaca acttctcgcc attgccgatg cgaacgggcg
     2341 cgccggtcgc ggtcttgacc tcgacaacag cgtttggggc tacggcttgg ggctgactca
     2401 cggcaagact tccggcgact agatgttttc aggccccaga caggtggcct cagaagcgag
     2461 atggtgcaag tgcggatcga cacggcaggc gacggaccca tcctggtgtg gcttcgcagg
     2521 gacctgcgcc tcactgacaa tccggccctg gatgcagcgg tttcgaccgg acgatcggtg
     2581 atcccgctct atatcctcga cgaaaccgag ggtgttcggc ccatgggcgc ggcgtcactg
     2641 tggtggctgg acaagtcgct ggcggccttg ggcgctgata tcgagcagcg cggcgggcga
     2701 ctgatcctgc gacgcggcaa ggctgctgaa atcctgaacc aactggtcgc cgagaccggc
     2761 gcagcgggcg tggtctggaa ccggctctac gacaaggcca gcatcgaccg cgacgcggct
     2821 atcaaggctg acctgcgcgg ccagggcctg acctgcgaga gcttcaacgc cagcctgctc
     2881 aatgagccct ggacggtgaa gaacggctcg ggcgaaggct acaaggtctt cacgccctac
     2941 tggcgggcgg ctcgtgagca tatcggcgag gtgcatgtgt cgcctgcgcc ggcagcc
//
LOCUS       DAOMEO010000306         2993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3647_length_2993_cov_2.382233, whole genome shotgun sequence.
ACCESSION   DAOMEO010000306 DAOMEO010000000
VERSION     DAOMEO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_2993_cov_2.382233"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLH31_07970"
     CDS             complement(<1..571)
                     /locus_tag="PLH31_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQR89187.1"
                     /translation="MVDLNAEMAELWSSLGAPAPGRPHVIQVVAARRGEGTSTIAREL
                     ARFTATRAGRKTWLVDMDLATSSQFQTIAMNPERFGILGPAVAASPDGSAFFTVQPPA
                     PRPDGGVWPDAKYLAAHSVGGSRLWVTRFNRDALRGRQKVHIIPGAEYWQALRRHAEV
                     IIVDSPSTDRSQAALTVAQHMDQTVMVVSA"
     assembly_gap    805..814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1227..2294)
                     /locus_tag="PLH31_07975"
     CDS             complement(1227..2294)
                     /locus_tag="PLH31_07975"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQR89188.1"
                     /translation="MEGQVWRHERVGGPAAEGAGGREPAAQVAVGRRHARQGGVERAA
                     LKKMVGPAAMREGVAHLRTVFEISERRACSIVKADRKMVRYRSRRPPDTKLRARLRDL
                     AAERRRFGYRRLFVLLRREGELSGKNRIYRLYREEGLAVRKRRARRRAIGTRAPILVE
                     AKANARWSLDFVHDQFAGGRRFRVLNVVDDVTRECLAAIPDTSISGRRVARELSALIA
                     RRGRPGMIVSDNGTEFTSTAILAWAEDHRVAWHYIAPGKPTQNGFVESFNGRMRDELL
                     NETLFFGLDHARQKLAAWAEDYNTRRPHSSIGYQTPAAYAAYLNATGRPAAQCGSSTA
                     RPVATTAPSGVTNAETLVRAG"
     gene            complement(2261..2413)
                     /locus_tag="PLH31_07980"
                     /pseudo
     CDS             complement(2261..2413)
                     /locus_tag="PLH31_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2472..>2993)
                     /locus_tag="PLH31_07985"
     CDS             complement(2472..>2993)
                     /locus_tag="PLH31_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQR89189.1"
                     /translation="IQTAPDNSIIRLSYQNEHPEMAEKVLNTLLEEYLTYRRGLLIGG
                     DDRVLQRQKDIFEQKLEETDSSYQSFLTTNDIGDFTSQKTTLTQLQTQVEQQKYSIEA
                     QLQDRVARLASVEAELARTPAETVLYRDNDPTAGLKLAQLKLDREGLLATWKPGSTPL
                     VEIDAKIAQLERG"
BASE COUNT          493 a          964 c          987 g          539 t
ORIGIN      
        1 gggcagacac caccatgacc gtctgatcca tatgctgagc gaccgtcagg gctgcctggg
       61 accgatccgt cgacggactg tcaacaatga tcacctcggc gtggcggcgc agcgcctgcc
      121 agtattccgc ccccgggatg atgtggacct tctggcgtcc gcgcagggcg tcgcgattga
      181 agcgggtcac ccagaggcgc gacccgccca cgctgtgcgc ggccagatac ttggcgtcgg
      241 gccagacgcc gccatcgggc cgaggcgccg gcggctgcac cgtgaagaag gccgagccat
      301 cgggggatgc agccacggcg gggcccagaa taccgaaacg ctcggggttc atggcgatgg
      361 tctggaattg cgaggacgtc gccaggtcca tgtcgaccag ccaggtctta cggccagcgc
      421 gcgtggcggt gaaccgggcc agttcgcggg cgatggtcga ggtcccctcg cctcttcggg
      481 cggcgaccac ctggatgacg tgcggacgcc caggcgctgg cgcgcccagt gagctccaca
      541 actccgccat ctcggcgttc aaatccacca tccgtcgaat cgccgcgcgt tcagggccta
      601 caaactaacg tcattcccct ggcgcgcgaa aggcgaccaa agcactcacc agacggggct
      661 caacgcctag gctgcgtgct tgtagccagc ggtggcgagc acaggcagat tcagggtccg
      721 cgcggctgag gctggggtct gcaagccggg gcgcaggaac atccgaacca aaccggcgca
      781 aagggcggta aaggcggcga acatnnnnnn nnnnggcgaa catgatcgtc agggccagga
      841 tcggcttttt caggctcttg ccttgcgacg gcgcgacggc gcgctggacg atgcggatat
      901 tgtcgctgct ctccgacgac atccggcgct gggcctcgtc ctgctgttcc ttgacggtga
      961 agtcgcgaac gttggaggtc agcacatcgc ggtcgcgggt cagctggttg aactgtggct
     1021 ccagctcggc caggcgcagc aggcgctggt tgacgtcggc gacctgctgg acggcggtcg
     1081 cctgggactc gcgtagcgcc gcgacttcgg cggcgagatc gttgcgcgag gtctgcaggg
     1141 tttgccagat cgggttggga ccactgcggc gcgcgccttc gcccgtggtg cgaccggcgg
     1201 agatgccctg acctgcctct gatctttcat ccagcgcgga ccagagtctc ggcgttggtt
     1261 acgcctgatg gcgcggttgt agcaacaggc cgagccgtgg agctcccaca ttgcgcagcg
     1321 ggtcggcctg ttgcgttcag gtacgcggca taggccgcag gggtctgata gccgatcgac
     1381 gagtgcggcc ggcgggtgtt gtagtcttcg gcccaggctg cgagcttctg gcgggcgtgg
     1441 tcgaggccga agaacagggt ctcgttgagc agttcgtcgc gcatccggcc gttgaaactc
     1501 tccacgaagc cgttctgggt gggcttgccc ggcgcgatat aatgccaggc gacgcgatga
     1561 tcctccgccc aggccagaat ggccgtcgag gtgaactccg taccgttgtc gctgacgatc
     1621 atccctggcc ggccacgacg agcgatcaac gccgacagct cgcgcgccac gcgccgaccc
     1681 gagatcgagg tgtcagggat cgccgccaga cattcccggg tgacgtcgtc gaccacattg
     1741 agaaccctga accggcgccc gcccgcgaac tggtcgtgca cgaagtccag cgaccagcgg
     1801 gcattggcct tggcctccac caggatcggc gcccgtgtgc caatggcgcg gcggcgagcc
     1861 cggcgtttgc gcaccgccag gccctcctcg cggtagagcc ggtagatgcg gttcttgccg
     1921 gaaagttcgc cctcccgacg cagcaggacg aacagccgtc gatagccgaa ccgccgtcgc
     1981 tcggcggcca ggtcacgcag tcgcgcacgg agcttggtgt ctggtgggcg gcgagaccgg
     2041 tagcgcacca tcttgcggtc cgccttgacg atggagcagg cccgccgctc gctgatctcg
     2101 aacactgtcc gcagatgagc gacgccctcg cgcatggcgg cgggccctac catttttttg
     2161 agagcagctc gttcaacgcc gccttgtcga gcatgacgtc tgccaacagc gacttgagcc
     2221 gccggttctc gtcctccagc gccttcagcc gctgggcctc cgacacgctc atgccgccaa
     2281 acttggcctt ccaggcgtag atcgtgcctt ccgacacccc gtgcttgcgg gccaactcac
     2341 ccgccttcgc cccggcctcg ctctcccgca ggatcccgat gatctgctct tccgtaaatc
     2401 tagctcgctt catgtccgtc ctctcaaggg ccggactcta gctccgcgtg gaggaaaaaa
     2461 tcaggggcag gtcagccccg ctccagctga gcgatcttgg cgtcaatttc gaccagaggc
     2521 gtagagcccg gcttccaggt ggccagcaag ccttcgcgat ccagtttcaa ctgggcaagc
     2581 ttgagccctg cggtagggtc gttgtcgcga tagagcacgg tctcggccgg cgtgcgcgcc
     2641 aactcggcct cgactgacgc cagacgagcg acgcgatcct gaagctgggc ctcgatggaa
     2701 tatttctgct gctcgacttg ggtctgcagt tgggtcagcg tggtcttctg cgacgtgaaa
     2761 tcgccgatgt cgttggtcgt cagaaacgac tgatagctgg agtcggtctc ctcaagcttt
     2821 tgctcgaaga tgtccttctg ccgctgcagc acgcgatcgt cgccgccgat tagcaggccc
     2881 cggcgatagg tcagatattc ctcgagcaga gtgttgagga ccttctcggc catctcggga
     2941 tgttcgttct gatacgacag ccgaatgatc gaattgtctg gcgcggtctg gat
//
LOCUS       DAOMEO010000307         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3653_length_2989_cov_2.841513, whole genome shotgun sequence.
ACCESSION   DAOMEO010000307 DAOMEO010000000
VERSION     DAOMEO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3653_length_2989_cov_2.841513"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="phnD"
                     /locus_tag="PLH31_07990"
     CDS             <1..866
                     /gene="phnD"
                     /locus_tag="PLH31_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR89190.1"
                     /translation="KPILVDMEKQTGLKVKPFFSSNYSSLIVAMGADQTDVGWFSNQS
                     GLEAVRRSGGEVFARTFDPSGTDGYKSVIIVPADSKLTLADLLKCDKTLDFGIGDKKS
                     TSGTLAPMTYVFIPAGKKPDTCFKTVLSASHDANLFAVANKKLDAATNNTTALRLNDN
                     RGGAQSNKIKVIWESPTLPEDPIIWRKDLDPVVKEKVRQFFLTYGQGDTPEAAQQRAN
                     LAKLSMGGFLAADDTHLLVVREMEALEQFGLAKETGDAAKIAAAQKALDGVKAERVAA
                     EGKAGLPAPAN"
     gene            875..1693
                     /gene="phnE"
                     /locus_tag="PLH31_07995"
     CDS             875..1693
                     /gene="phnE"
                     /locus_tag="PLH31_07995"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HQR89191.1"
                     /translation="MSSDTIPQPPKKSASALAFDILLWGGVIGLLVVSFRPAEIDKFP
                     QLFTEAGRMREFATGFFAPFGNAELFMSMDWPTYIGKMWQTIQMALWGTALAIIVAIP
                     LGLLGARNITPVWIQQPVRRALDLIRSLPDLVVALIFITAVGLGPFAGVMALMFNTGG
                     VLAKLFAEAVESIDKGPVEGVRATGAVPLQEIVWGVIPQVAPLWTSYALYRFESSSRS
                     ATVLGLIGAGGIGQVLLESVNGFQYDQTGCIVLVIVVAVSMIDLLSQIIRTRLL"
     gene            1943..>2989
                     /locus_tag="PLH31_08000"
     CDS             1943..>2989
                     /locus_tag="PLH31_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQR89192.1"
                     /translation="MILLALVVVFFLVLLNGVFSMSELAVVSARRARLQTYADRGDKG
                     AKLALAMAEHPTRFLSAVQVGITLIGILAGAYGQATIAGALDLWLEPMPVVGPHSEII
                     ATIVVVIGLTYVSVILGELVPKRLALLFPDAIARRMAPFLAIVATVLRPFVTILTVST
                     AAILKLMGVRDERTNDMTAEEVETVLAEGADAGLIEPEERSMIQEVLRLGDRPVRVAM
                     TPRRDLFWISLADTPEEILAEIRSCRYSRIVVATDDDLDGDIGVVLKKDLLDAALSGG
                     PIDVLAHVQQPIAIPETMSLLRAMALFKQTPLHMALVVDEFGSVQGVITPIDLLEMIA
                     GDFPESHDDTETRIL"
BASE COUNT          485 a         1030 c          957 g          517 t
ORIGIN      
        1 ggaagcccat cctggtcgac atggaaaagc agaccggcct gaaggtgaag ccgttcttct
       61 cgtccaacta ctcgtcgctg atcgtggcca tgggcgcaga ccagaccgat gtgggctggt
      121 tctccaacca gtcgggcctg gaagcggtgc gccgctcggg cggcgaagtg ttcgcccgca
      181 ccttcgatcc gtcgggcacc gatggctaca agtcggtgat catcgtcccg gccgacagca
      241 agctgaccct ggccgacctg ctgaaatgcg acaagacgct ggatttcggc atcggcgaca
      301 agaagtcgac ctcgggcacg ctcgcgccga tgacctatgt gttcatcccg gccggcaaga
      361 agcctgacac ctgcttcaag accgtgctga gcgccagcca tgacgccaac ctgtttgcgg
      421 tggccaacaa gaagctggat gcggccacca acaacaccac ggccctgcgc ctgaacgaca
      481 atcgcggcgg ggcccagtcc aacaagatca aggtgatctg ggaatcgccg accctgccgg
      541 aagacccgat catctggcgc aaggacctgg acccggtggt caaggaaaag gtccggcagt
      601 tcttcctgac ctatggccag ggcgacaccc cggaagcggc ccagcagcgc gccaatctgg
      661 ccaagctgtc gatgggcggc ttcctggccg ccgacgacac ccacctgctg gtggtgcgcg
      721 aaatggaagc cctggagcag tttggcctgg ccaaggaaac cggcgacgcc gccaagatcg
      781 ccgccgccca gaaggccctc gacggggtca aggccgagcg cgtcgccgcc gagggcaagg
      841 ccggcctgcc cgcgcccgcc aactgagcct gttcatgagc agcgacacca tcccgcagcc
      901 gccgaagaag tcggcctcgg ccctggcctt cgacatcctg ctgtggggcg gggtgatcgg
      961 cctgctggtc gtcagcttcc gaccggccga gatcgacaag ttcccgcagc tgtttaccga
     1021 agcgggccgg atgcgcgagt tcgccaccgg cttcttcgcg ccgttcggca atgccgagct
     1081 gtttatgtcg atggattggc cgacctatat cggcaagatg tggcagacca tccagatggc
     1141 gctgtggggc accgccctgg ccatcatcgt cgccatcccg ctgggcctgc tgggcgcgcg
     1201 caacattacc ccggtgtgga tccagcagcc ggtgcgccgg gcgctggacc tgatccgctc
     1261 gctgcccgac ctggtggtgg ccctgatctt catcaccgcc gtgggcctgg ggcccttcgc
     1321 tggcgtgatg gccctgatgt tcaacaccgg cggcgtgctg gccaagctgt tcgccgaggc
     1381 cgtcgagtcg atcgacaagg gcccggtcga gggcgtgcgc gccaccggcg ccgtgcccct
     1441 gcaggagatc gtctggggcg tgatccccca ggtggcgccg ctgtggacca gctacgccct
     1501 gtaccgcttc gagtcgtcca gccgctcggc caccgtgctg gggctgatcg gggctggggg
     1561 cattggccag gtgctgctcg aatcggtcaa cggcttccag tacgaccaga ccggctgcat
     1621 cgtgctggtc atcgtggtgg cggtgtcgat gatcgacctg ctgtcgcaga tcatccgcac
     1681 ccgcctgctc tgacgcgctt tcagggcgcc taaatcacgc tcgacaggcc ttacatcgcc
     1741 ccagccctat gggccggggc gatttctttt gtatagtggg ccacggttca ccctcgccgc
     1801 gtcaaggaag cagggtcgcg ctcgccgtcc ggcgtcgcgt cattgacccc ccaaccgttt
     1861 gctttgcaaa cggccgggcc aaaaagggaa agcgcgtcct gctcgcgaaa ccgcctacac
     1921 tttcgcgggc cgcgctgcgg ccatgatcct cctcgcgctc gtcgtcgtct tcttcctcgt
     1981 tctgttgaac ggggttttct ccatgtctga actggcggtg gtttccgccc gccgggccag
     2041 gctccagacc tatgccgatc gcggcgacaa gggcgccaag ctggccctgg ccatggccga
     2101 gcatccgacc cggttcctgt cggccgtgca ggtgggcatc accctgatcg gcatcctggc
     2161 cggcgcctat ggccaggcca ccatcgccgg ggccctggac ctgtggctgg aacccatgcc
     2221 ggtcgtcggg ccccattcgg agatcatcgc caccatcgtc gtggtcatcg gcctgaccta
     2281 tgtgtcggtg atcctcggcg agctggtgcc caagcgcctg gccctgctgt tccccgacgc
     2341 catcgcccgc cgcatggcgc cgtttctggc gatcgtggcg acggtgctgc ggcccttcgt
     2401 gaccatcctg accgtctcga ccgccgccat cctgaaactg atgggcgtac gcgacgagcg
     2461 caccaacgac atgaccgccg aagaggtcga gaccgtgctg gccgaaggcg ccgacgccgg
     2521 cctgatcgag cccgaagagc gctcgatgat ccaggaagtg ctgcgcctgg gcgaccgccc
     2581 ggtgcgcgtg gccatgaccc cgcgccgcga cctcttctgg atctccctgg ccgacacgcc
     2641 cgaagagatc ctggccgaga tccgcagctg ccgctattcg cgcatcgtgg tcgccaccga
     2701 cgatgatctg gacggcgata tcggcgtggt cctgaagaag gacctgctgg acgcggctct
     2761 gagcggcggt ccgatcgacg tgctggccca tgtgcagcag cccatcgcca tccccgagac
     2821 catgtcgctg ctgcgcgcca tggccctgtt caagcagacc ccgctgcaca tggccctggt
     2881 ggtcgacgag tttggctcgg tgcagggggt gatcacgccc atcgacctgc tggaaatgat
     2941 cgccggcgac ttccccgaaa gccatgacga caccgagacc cgcatcctg
//
LOCUS       DAOMEO010000308         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3659_length_2983_cov_3.141393, whole genome shotgun sequence.
ACCESSION   DAOMEO010000308 DAOMEO010000000
VERSION     DAOMEO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3659_length_2983_cov_3.141393"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2742
                     /locus_tag="PLH31_08005"
     CDS             <1..2742
                     /locus_tag="PLH31_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89193.1"
                     /translation="VREVNAIRGSLPSGIARLDIQRARTTEVAIVQVALTSDVLPMRR
                     LEKTADRLRERLDRVPGVREARYWGAPSSEVQVTLDLARLSALRLPATAVADALRQAG
                     AEAPIGAVQAGERRFNVKSGGAFRSLEAVGDTPVRSVGGQVTRVRDVAKVAWAQAEPT
                     HLARFNGRRAVFLTVTQKDGQDVARITKSVEQVLDEYEKILPAGVKLQRGFVQADNVK
                     HRLNALFRDFGIALVLVLITLLPLGPRAGLVVMVSIPLSLLIGLSMLQAFGFTLNQLS
                     IAGFVLSLGLLVDDSIVITENIARRIREGEERTEAAVNGTRQIGLAVIGCTATLMLAF
                     LPLMALPAGSGAYIKSLPVTVLCTIAASLLVSMTIIPFLASRILDKHSDPEGNALLQA
                     VNGGIHRFYRPVLHRALARPWVALAIMLALCLTTYPLVKLIGSSLFPPAETPQFLVRI
                     ETPDGTSLARTDRALKFVEQRLKATPEVTWRAANLGRGNPQIFYNQQQRESATTYAEV
                     FVSLKAWEPGRSDQVLDGLRRDFSNFPGARISVVTFENGPPIDAPIAIRLTGQNLDAL
                     KALAARAEATLKATPGTRDVTNPVRLDRTDLDLGVDEAKASALGVPAGAARRVARLAL
                     SGEETARFRDPDGDDYAVKVRLPMEQADGAARNPLSALKGVYVPTADGLSTPLDAVAT
                     PTLKSSPARIDRFDRERTVTVTSYVATGFLAAKVTEDVVARLEKDLPLPPGYRLTLGG
                     QAEAQSESFAGLGAAVLIAVFGILAVLVLEFQKFKTALVVAGIIPFGIFGAVAALAIT
                     GNSLSFTATIGLIALIGIEIKNSILLVDFTEQLRREGMGLHDAIEKAGEVRFLPVLLT
                     SVTAIGGLLPLALERSGLYSPLAIAIIGGLITSTVLSRVATPVMYWLTARGDERATA"
BASE COUNT          451 a         1073 c          967 g          492 t
ORIGIN      
        1 gtacgcgagg tgaacgccat tcgcggcagc ctgcccagtg gcattgccag gctcgatatc
       61 cagcgggccc gcaccaccga ggtcgctatc gtccaggtcg ccctgaccag cgacgtgctg
      121 cccatgcgtc gcctggaaaa gaccgctgac cgcctgcgcg agcgactgga ccgggtgccc
      181 ggcgtacgcg aggcccgata ttggggcgcg ccgtcgtccg aggtgcaggt caccctggat
      241 ctcgcccgcc tgtcggccct gagactgccc gccaccgccg tcgccgacgc cctgcgccag
      301 gctggcgccg aggcccctat cggcgcggtc caggccggag agcgccgttt caacgtcaag
      361 tccggcgggg ccttccgctc gctggaagcg gtgggcgaca cgccggtgcg ttccgttggc
      421 ggccaggtga cccgcgtgcg cgacgtggcc aaggtggcct gggcccaggc cgagcccacc
      481 catctggccc gtttcaacgg ccggcgcgcc gtgttcctga ccgtaaccca gaaggacggc
      541 caggacgtcg ccaggatcac caagagcgtc gagcaggtgc tggacgagta tgaaaagatc
      601 ctgcccgccg gcgtgaaact gcaacgcggc ttcgtccaag ccgacaacgt caagcatcgc
      661 ctgaacgccc tgttccggga tttcggcatc gccctggttc tggtgctgat caccctgctg
      721 ccgctgggtc cgcgcgccgg gctggtggtg atggtctcga tcccgctgtc cctgctgatc
      781 ggactgtcga tgctgcaggc cttcggcttt acgctcaacc agctgtccat cgccggtttc
      841 gtgctgtcgc tgggcctgct ggtcgatgac agcatcgtca tcaccgagaa catcgcccgc
      901 cgcatccgcg agggcgagga gcgcaccgag gccgccgtca acggcacgcg ccagatcggc
      961 ctggcggtga tcggctgcac ggccaccctg atgctggcct tcctgccgct gatggccctg
     1021 cccgccggct cgggcgccta tatcaagtcg ctgccggtca ccgtgctgtg cacgatcgcc
     1081 gcctcgctgc tggtctcgat gaccatcatt ccgttcctgg ccagccgtat cctcgacaag
     1141 cattcggacc ccgaaggcaa cgccctcctg caggcggtca atggcggcat tcaccggttc
     1201 tatcgcccgg tgctgcatcg ggcccttgcc cggccgtggg tggccctggc gatcatgctg
     1261 gccctgtgcc tgaccaccta tccgctggtg aaactgatcg gctctagcct gttcccgccc
     1321 gccgagaccc cgcagttcct ggtgcggatc gagacccccg acggcacgtc cctggcccgc
     1381 accgaccgcg ccctgaagtt cgtggaacag cgcctgaagg cgacccctga agtcacctgg
     1441 cgcgccgcca atctgggccg cggcaatccg cagatcttct acaaccagca gcaacgcgag
     1501 agcgccacga cctatgccga ggtgttcgtc agcctcaagg cctgggagcc ggggcggagc
     1561 gaccaggtgc tggacggcct gcggcgcgac ttcagcaact tccctggcgc gcggatcagc
     1621 gtcgtcacct tcgagaacgg cccgccgatc gacgcgccga tcgccatccg tctgaccggt
     1681 cagaacctcg atgcgctcaa ggccctggcc gcccgcgccg aggcgaccct gaaggccacc
     1741 cccggcacgc gcgacgtgac caaccccgtc cgcctggacc gcacggatct tgacctcggc
     1801 gtcgatgagg ccaaggcctc ggccctgggc gtaccggccg gcgcagcccg gcgcgtggcc
     1861 cgcctggccc tgtctggcga ggagaccgcc cgcttccgcg acccggacgg cgacgactat
     1921 gcggtcaagg tgcggctgcc gatggagcag gctgacggcg cggcgcgcaa tccgctttcg
     1981 gccctcaagg gggtctatgt gcccaccgcc gacggccttt ccacgccgct ggacgctgtc
     2041 gccacaccga ccctgaaatc cagccccgcc cgcatcgacc gctttgaccg cgagcggacg
     2101 gtgaccgtca cgtcctatgt cgccaccggc ttcctggccg ccaaggtgac ggaggacgtc
     2161 gtcgcgcggc tggaaaagga cctgccgctg ccgcccggct atcgcctgac cctgggcggc
     2221 caggccgagg cccagtcgga gagcttcgcc ggcctgggcg cggcggtgct gatcgcggtg
     2281 ttcggcatcc tggcggtact ggtcctggaa ttccagaagt tcaagacggc cctggtcgtg
     2341 gccggcatca ttccgttcgg catcttcggc gcggtggcgg ccctggctat caccggcaat
     2401 tcgctgagct tcaccgccac gatcggcctg atcgccctga tcggtatcga gatcaagaac
     2461 tcgatcctgc tggtcgactt caccgaacag ctgcgccgcg agggcatggg tctgcacgac
     2521 gccatcgaaa aggccggcga ggtgcggttc ctgcccgtgc tgctgaccag cgtcacggcg
     2581 atcggcgggt tgttgccctt ggcgctggaa cggtcgggtc tgtactcgcc gctggccatc
     2641 gcgatcatcg gcgggctgat cacctcgacc gtactgtcgc gggtggcgac gccggtgatg
     2701 tactggctga ccgcgcgggg ggatgagcgg gcaaccgcat agatcctccc cctgaagggg
     2761 gaggcggccg gaggccggtg ggggtagtcg gcaatgactt ggccgtcact tccccctccg
     2821 atcgcttcgc gatcccctcc cccttcaggg ggaggactta gatccgactt gcctattata
     2881 cactcgtata gcaacctctc cccacacatc ggggaacaaa ccacatggcc gcaacccaaa
     2941 cccgcgacgc cgtcattatc ggcgggggac acaacggcct cgt
//
LOCUS       DAOMEO010000309         2974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3665_length_2974_cov_2.377869, whole genome shotgun sequence.
ACCESSION   DAOMEO010000309 DAOMEO010000000
VERSION     DAOMEO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_2974_cov_2.377869"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..844
                     /locus_tag="PLH31_08010"
     CDS             623..844
                     /locus_tag="PLH31_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89194.1"
                     /translation="MVSMGMAQAVYQHAHDFYDTGSWYVVAECWDLLAIAEELDRQEE
                     ITAIPFELDTAAIAHFHQMIHARTGGTRH"
     gene            1666..2034
                     /locus_tag="PLH31_08015"
     CDS             1666..2034
                     /locus_tag="PLH31_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR89195.1"
                     /translation="MLNKVQLIGFTGAEAEIRTTSGGKKVATLRVATNRYTKAGGERK
                     DYTTWHQVEIWNQGTVGWLGSKGLPKGSKVFVEGEIRHDRYTDKDGVERFFSKVVVAT
                     PQHELRSLDRPDTAPASEEE"
     gene            2125..>2974
                     /locus_tag="PLH31_08020"
     CDS             2125..>2974
                     /locus_tag="PLH31_08020"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HQR89196.1"
                     /translation="MVNGTLTLGAESSSNSWPGCLSLSKRDPMSTVKPRQIELFVAQQ
                     LEAPSLRDDIDIMSLPFFSIEKTPRHDPIEFERVVGDRRQYIRVSPGEFGLATIWDND
                     IILYLRTQIIEALNRNRDVSKRISFHIHDCLVATGRRTGGREYELFKEALTRLKTTTV
                     LTNISTSDEIADEGFGWLESFKIKRRRTAAGADVMASCEVVISDWLFGLFLNSRRALA
                     IDSAYFHLQGGLERKLYGILRKHLGQQSMWQIGTDTLKDLCGVKRDVRRFTYDLKKIV
                     ERGLPGF"
BASE COUNT          498 a          982 c          979 g          515 t
ORIGIN      
        1 cgccgcaccc gccttccggc gggtgaaagg gtggaggtgc gctgcgcttc cgcttcttag
       61 cttcctgtcg ggaatacccc tatcgccggc gtcccgcctc gggcggcgcc gcgggtcgct
      121 gtcgtacaga tcgccaagtg atgggtctca gcgccaccaa tcgccgattt tggcgagcgc
      181 gtgcctgtcg gtgcggaggt ggtgagaacc gatgggtgca ggcggcgggc ccggcgctgc
      241 gccgttcacc tgcaggatgt tgccatcgcc agcgcctgtg agggttccgc gagggggcgc
      301 ccagagcgct gagggcgtca ggggtctggc tgtggtgatc cgcatgtcgg cgccagcacc
      361 aagccgccag tcgtctcccg ggttgggcgc cgcggggtcg aactcgtccg cgatcaatct
      421 cacgaaccta ggacgggacg gtcagggtca aaggcgacct cgcctgagcg aggcgaaccc
      481 cgcggaccct ccgaccttcg ctgcgctgca ggccggtttc cccggggttc gtcctttggc
      541 ccctcccttt cacccgccct gggcggtcgc gagatcgcgg gaacgagccc gcagatccag
      601 cagcgcaaga gggagacgaa agatggtcag catggggatg gctcaagcgg tttatcaaca
      661 cgcccacgac ttctacgaca ccggcagctg gtatgtggtc gccgaatgct gggaccttct
      721 ggccatcgcc gaggagctgg accgccagga ggagatcacc gcgatcccct tcgagctcga
      781 cacggccgcc atcgcccact tccaccagat gatccacgcg cggacgggcg gcacgcggca
      841 ctgacctaac tcggcgcccg ctccggcggg cgccgttccc cctgcccttc cccaacttgg
      901 atgaagccgg tggaacaggg gtcgaacgaa atccaagtcc ttgtgtcagg cagaccggcg
      961 gccactcagc cttggggttg gtgattcgtt ctgacgtcga gccttggagg cctcgaacgg
     1021 gtgctttgat tgggcgagat tgaagccagc ggcccgcccg gcgagccggg ctgggccgag
     1081 cgcatctccg agatgccgaa gctggcctgg aaggtctgcg atgtgcgtat gcggtaggcc
     1141 acggacctgg ccgcccgacc cccgtgcctt acggttcttc ggtcagccat ctccccgact
     1201 ggacgaacgc agcgatcctt tctccccctg ggcgggccag ccccgcaact ggttccagaa
     1261 cggccaggta ggccgagttc agccccctcc cctcgcccgc ctcgcggtcg tcaatcggag
     1321 gcgtcggcgg gcggcgaccc ttgccttgtg ttcaacgatc cgcgtcgccc cgtgccctcg
     1381 tggtccttgt tctgatcatc tccaagccgt tgtgacgccc ggcgggctgc ggtccgcgcg
     1441 atcaatcact tcaacctagg tcggggcggt ccaggtcaaa gccgaccccg ctgcgcgcgg
     1501 cggaccggat ggaccctccg gccttcctcc gctgcgctcc gtgcaggccg gtttccaacc
     1561 ggaccgggct ttgccctgtc cctcatcccc gcccttgcag gtcgcgtgat cgcggcaggg
     1621 accgcgagcc gccgggattt ccctggcggc agatggagta agacgatgct gaacaaggtc
     1681 caattgatcg gtttcaccgg agccgaagcg gaaattcgca ccacgtcagg cggaaagaag
     1741 gtcgccaccc tgcgggtcgc cacgaaccgc tacacgaagg cgggcggcga gcggaaggac
     1801 tacacgacct ggcaccaggt cgagatctgg aaccagggca cggtgggctg gctcgggtcc
     1861 aaggggctgc cgaagggctc gaaggtgttc gtcgaggggg agatccggca cgaccgctac
     1921 accgacaagg acggtgttga acggttcttc tccaaggtcg tggtggccac gccgcagcac
     1981 gaactgcggt cgctggaccg gcccgacacc gctccggcct ccgaggagga gtagctcaag
     2041 cgcccgcccg gttcgccggg cgggccgctc ttgccggttg gcggtcgcgc ggctcaagcg
     2101 cctgtcggcc gggtctggcg gggcatggtt aacggcacct taacacttgg cgcggaatca
     2161 tcgtcgaact cgtggcctgg atgcctgagt ctgagtaagc gagaccccat gagcacggtg
     2221 aaacccaggc agatcgaact cttcgttgct cagcagctcg aagcgccgtc gctccgcgac
     2281 gacatcgaca tcatgtccct gcccttcttc tcgattgaga agacaccgcg tcacgacccc
     2341 atcgagttcg agcgtgtcgt tggcgaccgt cgtcaataca tccgggtctc gccgggggag
     2401 ttcggcctgg ccacgatctg ggacaacgac atcattcttt atctgcggac gcagatcatc
     2461 gaggcgctga accgcaaccg cgatgtcagc aagcggatct cattccacat ccatgactgc
     2521 ctggtggcga ccggacgccg gaccggcggc cgggaatacg aactcttcaa ggaggcgctg
     2581 acgcgcctga agaccaccac agtcctgacc aacatatcga cgtcggacga aatcgccgac
     2641 gagggctttg ggtggctgga gagttttaag atcaagcggc gtcgcacggc cgcgggcgcc
     2701 gacgtgatgg cgtcctgcga ggtggtgatc tccgactggc tattcgggct cttcctcaac
     2761 agccgccgcg cgctggccat cgatagcgcc tacttccatc tgcagggcgg attggagcgc
     2821 aagctctacg gcatcctgcg taaacatctc ggccagcaat cgatgtggca gatcggcacc
     2881 gacaccctga aggatctttg cggcgtgaag cgcgacgtgc ggcgcttcac ctacgatctg
     2941 aagaagatcg tcgagcgtgg ccttcctggc ttcg
//
LOCUS       DAOMEO010000310         2973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3668_length_2973_cov_2.255655, whole genome shotgun sequence.
ACCESSION   DAOMEO010000310 DAOMEO010000000
VERSION     DAOMEO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_2973_cov_2.255655"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLH31_08025"
     CDS             <1..542
                     /locus_tag="PLH31_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR89197.1"
                     /translation="FAMGFVFVFTMGGVTGVVLANAGIDYSLHDTYYVVAHFHFVMAL
                     GAAFAIFAAIYYWLPKMIGRMIPEWAAVVHFWTFFVGSVITFFPQHFLGLQGMPRRYV
                     DYPDAFALWNYVSSLGAFLAHGSFVWFLGLMAWTVFAGRRAPDNPWGAGATTLEWTLS
                     SPPPYHAFETLPVVKADEH"
     gene            542..1117
                     /locus_tag="PLH31_08030"
     CDS             542..1117
                     /locus_tag="PLH31_08030"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HQR89198.1"
                     /translation="MRAGPTAPGSRSRPGVVPTIVVGSRPASPASDEGRAEPSLSNRT
                     PTVIELTERRSAPLRTAVAIVVLFCGFALLSTLRFPRPVLWPILGAVAIYLLLFLTLS
                     VAFLTRPRTRERIEIDGSRLTIELSSGRSLVRREWRTPWTVLDAYDDEEDDVRLWLVS
                     GDQRVAIGRHVGAQARRDAITSLQACLPGGV"
     gene            complement(1141..2292)
                     /locus_tag="PLH31_08035"
     CDS             complement(1141..2292)
                     /locus_tag="PLH31_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR89199.1"
                     /translation="MLDRFKIRRTPAIALGVILLVGGVTAVALTARHQPETTSTASAS
                     PTVVVVRPERTAADALSLPVRLAPHNLAQIHARTAGYIDRWYVDIGQAVTAGQMLARL
                     NAPELDQQIGQATADLQTAQANLAIASSTAERWALLFEKDLVARQAYEERTAARDAAL
                     SMRDSAAANVARLRTLQGYTRIRAPFAGVITDRQAQVGALVNAGATTEPLFTLADIRQ
                     LRIVAHVPQRQIGQLNEGLQANLTLPEYPGQTFSAVLARSSGAIEPQSGAMRVEFVVD
                     NSDGRLRPGSYGNLQLSKPPLGEGLMVPGSSLISTRGATQVAVVTAQGRVDLRPVKVG
                     RDNGQTVEIVDGLTPADRVILTPSDGVRQGEAVTPRLRPASRRAEATAG"
     gene            complement(2285..>2973)
                     /locus_tag="PLH31_08040"
     CDS             complement(2285..>2973)
                     /locus_tag="PLH31_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89200.1"
                     /translation="IQPVINIYAGVQGRDLGAVANGIEAAIARTADAVPDGSTVRLAG
                     QYETMNTAFSGLAIGLLGAIVLIYLIIVVNFQSWADPLVIIAALPGALAGIVWTLLLT
                     GTTLSVPALIGAILCMGVATANAILVVSFARERLAETGNALGAALDAGLVRFRPVLMT
                     ALAMIIGMAPMALGIGEGAEQNAPLGRAVIGGLVVATVATLILVPTLFAVAHRRARPR
                     QLPLGESRGA"
BASE COUNT          491 a          973 c          965 g          544 t
ORIGIN      
        1 tttttgcaat gggcttcgta ttcgtcttca ccatgggcgg ggtgaccggc gtcgtcctgg
       61 ccaatgcggg catagattac agcctgcacg acacctatta tgtcgtggcg cacttccact
      121 tcgtgatggc gcttggcgcg gccttcgcca tcttcgcggc catctactac tggcttccca
      181 aaatgatcgg ccggatgatt ccggagtggg cggctgtggt gcatttctgg accttcttcg
      241 tcggctcggt gatcaccttc ttcccgcagc atttcctcgg actgcagggc atgccgcgcc
      301 gctacgtcga ctacccggac gcctttgcgt tgtggaacta cgtttcgagc ctcggcgcct
      361 tcctcgctca cggctccttt gtctggttcc tcgggctgat ggcctggacg gttttcgccg
      421 gacgccgcgc gcccgacaat ccgtggggcg cgggagccac gaccctcgag tggacgctgt
      481 catcgcctcc tccctatcat gcgttcgaga ccttgccggt cgtcaaggcg gacgaacact
      541 gatgcgggcg ggaccgaccg cgccgggatc gaggtcccgc cccggcgtcg tcccaacgat
      601 cgtcgtcggg tcgcggccgg cgtctccagc cagtgacgag ggtcgcgccg agccgagcct
      661 ttccaaccga acgccgaccg tcatcgaact gaccgaaagg cggtcggcgc cgttgcggac
      721 ggccgtcgcc atagtcgttc tgttttgcgg gttcgccctg ctctccacgc ttcgctttcc
      781 ccggcccgtg ctttggccga tcctgggcgc ggtcgcgatt tatcttcttc tctttctgac
      841 gctttccgta gcctttctaa cccgcccgcg aacgcgcgaa cggatcgaaa tcgacggctc
      901 aaggctgacg atcgaactgt ccagtggccg gtccctggtg cgacgggagt ggcgcacgcc
      961 ttggaccgtt ctggacgcct atgacgatga agaggacgat gtcaggctct ggctggtcag
     1021 tggtgatcaa cgggttgcga tcggccgaca tgtcggtgcg caagcccgtc gtgacgcgat
     1081 cacaagcctc caggcctgtc tgcccggcgg ggtgtagccc tgcactatgc caagagacgc
     1141 tcagcccgcc gtcgcttccg ctcggcgcga cgcgggcctt aggcgcggcg tcaccgcttc
     1201 tccctgacgc acgccgtcgg agggggtgag aatgacgcgg tccgcagggg tcaagccatc
     1261 gacgatctcc acagtctgac cattgtcgcg ccccaccttc acaggtcgca gatcgacgcg
     1321 cccttgggcc gtcaccaccg cgacctgagt tgcgccccgc gtcgaaatca agctggatcc
     1381 gggcaccatc aaaccttcgc ccagtggcgg cttgctgagc tggaggtttc cgtaactgcc
     1441 gggccggagg cgtccgtcag aattgtccac gacaaactcg acccgcatgg ctccactctg
     1501 cggctcgata gcgcctgaag aacgtgccag cacggccgaa aacgtctggc ccggatattc
     1561 tgggagcgtc aggttcgcct gcaggccctc gttcagctga ccgatctggc gctgcggaac
     1621 atgcgccacg atccgcagct ggcggatatc ggcaagggtg aaaagcggct cggttgtcgc
     1681 gccggcattg accagggcgc cgacctgggc ctgacggtcg gttatgaccc cggcaaaggg
     1741 cgctcgaata cgcgtgtaac cttgcagagt ccggagtctg gccacattgg ccgccgcgct
     1801 atcgcgcatg gacaaggcgg cgtcgcgcgc cgccgtccgc tcctcatagg cttggcgagc
     1861 caccaagtct ttttcgaaca gcagcgccca tcgctcggct gttgaagacg cgatggccag
     1921 attggcctgc gccgtctgaa gatcggccgt ggcctggccg atttgctgat cgagttcagg
     1981 cgcgttcaac ctcgccagca tctgtcctgc cgtgaccgct tggccgatat ccacatacca
     2041 gcggtcgata tagccggccg tccgggcgtg gatctgggca aggttgtggg gggccaggcg
     2101 gacaggcagg ctgagcgcat cggcggcggt gcgctccggc ctgacgacca ccacggtcgg
     2161 ggacgcggac gcggtcgacg tcgtttcggg ttgatgacgc gccgtcaggg cgacggccgt
     2221 gacgccgccg accagcaaaa tgaccccgag tgcgattgca ggggtgcgac gaatcttgaa
     2281 tcgatcaagc accacgagac tctcctagag gcagttgccg cgggcgggcc cgacgatggg
     2341 ccacggcgaa cagggtcgga acgaggatta gggtcgcaac ggtcgcgaca acgaggccgc
     2401 caatgacggc ccgtcccagc ggagcgttct gttcggcgcc ttcgccgatc ccgagcgcca
     2461 tgggcgccat gccgatgatc atggccaagg cggtcatcag cacgggtcgg aaccggacga
     2521 gaccggcgtc aagcgccgcg cccagggcat tgcccgtctc cgccagccgt tcgcgcgcga
     2581 aactgacgac cagaatggcg ttcgcggtcg cgacgcccat gcacaggatg gcgccgataa
     2641 gagccggcac cgaaagggtg gtgccggtca gcaggagggt ccagacaatg ccggcgagtg
     2701 cgccaggcaa ggctgcgatg atgacgagcg gatcggccca actctggaag ttcaccacga
     2761 tgatcagata gatcagcaca atcgccccca gcaggccgat cgcgagtcct gagaaggcgg
     2821 tattcatcgt ctcgtactgt ccggcgagcc gaaccgtcga gccgtccggc accgcgtcgg
     2881 cggtccgcgc gatggcggct tcgatgccgt tcgccaccgc gccgagatca cgaccctgca
     2941 cgccggcgta gatgttgatc acgggttgaa tat
//
LOCUS       DAOMEO010000311         2965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3683_length_2965_cov_2.439863, whole genome shotgun sequence.
ACCESSION   DAOMEO010000311 DAOMEO010000000
VERSION     DAOMEO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3683_length_2965_cov_2.439863"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLH31_08045"
     CDS             complement(<1..804)
                     /locus_tag="PLH31_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HQR89201.1"
                     /translation="MNAMLPLFATAAASDPASHKAANVLAAARALTPQLNRSRALDRR
                     LVANTMTMAFGGSDAEGAWIWRDAYDAVEAALVLQLRRLSPQISRLEDAPAEICALLA
                     NLADLGLTHTRRSEEQVALDQFSTPPQLGALAVLAAQIRPGDQVLEPSAGTGLLAVLA
                     EACGGCVTLNEIAGQRGAILEGLFPLAARSAHDARHLKDILPASGNFHAVVTNPPFQV
                     LEDHLHAALDTLADGGRLSAIVPTRLFEDAAAMRRLAARGRQRGGHSPWR"
     gene            complement(938..>1755)
                     /locus_tag="PLH31_08050"
     CDS             complement(938..>1755)
                     /locus_tag="PLH31_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR89202.1"
                     /translation="FGGASNDVEVPRADVEVEGASHVLHETRTDVATRGLIRDLADDP
                     SAALTVLVAQLFKQLALHSSGALDGSASQIAATRYQRASQTPIAALDGEVRGRLDARR
                     EAYRASGLRPITWVETLPHGEKMALLAELTAVSLNIREARTSLLRHGARAEAAEIAAL
                     CGADISAHWTPDEAYLAVHSKAQLLVMLDELEVEDDRAKTLKKDALVTFVAEAAAERQ
                     WAPAVLSWDRPAALDETDDEAPTEEDAGAAEADSSADPEAEPGAEPLADAIAA"
     assembly_gap    1756..1765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1766..2923)
                     /locus_tag="PLH31_08055"
     CDS             complement(<1766..2923)
                     /locus_tag="PLH31_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR89203.1"
                     /translation="MTDQSPTSPVAMSRIQVRLGDLGLAKENLRFHEPADDGVPQLAD
                     TLLAAGVVIPPIVRPGRKGEALFMALDGRRRRFGLILLRECGEIDDDYRIDCLLAETP
                     AQQAAAIVLPNTEHAPVHIADVITAIGKLRKAKMDTQAISTALGYAELEIKRLEALAA
                     VHPTVLKALRQGRLTLKQVRLFARLPDKKQQAEIAQTALDGYFQDYQLRAVVEHDRAT
                     VDDDRFTLVGMDRYLAAGGRVASDLFGEFPDFLLDPEVLQGAWRERVQPIVDHLKAEG
                     LAVFVGREPGYSAPDGFFRLPHVWERDLDEGQKAALSDARYRVTQLESALQDLDPSTE
                     EAPAQLAPLVSAFGVAAGAVLTRSKIGAVLLTPSDGHGLAGTFFSVPLPIED"
BASE COUNT          457 a         1021 c         1038 g          439 t
ORIGIN      
        1 gcgccatggt gaatgaccgc cccgttgtct cccccgcgcc gccagacggc gcatggctgc
       61 ggcatcctcg aacagccgcg tcggcacgat cgccgagagc cgcccgccgt cggccaaagt
      121 gtcgagggcg gcatgcaggt ggtcttccag cacctggaag ggcgggttgg tgacgaccgc
      181 gtggaagttg ccggacgcgg gcaggatgtc cttcagatgc cgggcgtcat gggcgctgcg
      241 agcagccaag ggaaacaatc cctccaggat cgcgccgcgt tgaccggcga tctcgttcag
      301 agtcacgcat ccgccgcagg cctcggccag cacggctaaa aggccggtgc cggcggacgg
      361 ctccaggacc tggtcgcccg gccggatctg ggctgccagc accgccaggg cgcccaactg
      421 cggcggcgtc gagaactggt ccagcgcgac ctgctcttca ctgcggcgcg tgtgggtgag
      481 gcccaggtcg gcgagattgg ccagcaaggc gcagatctcg gccggcgcat cttccagccg
      541 gctgatctgc ggcgacaggc gacgcagctg cagaaccaaa gccgcctcga cggcgtcata
      601 ggcgtcgcgc cagatccacg cgccttcggc gtcagagccg ccgaaggcca tggtcatggt
      661 gttggccacc agccggcggt cgagcgcccg agagcggttc agctgggggg tcagggcccg
      721 ggcggcggcc agaacattgg cggccttgtg tgacgccgga tcggaggcgg cagctgtggc
      781 gaagaggggg agcatggcgt tcatgacggg gattccgctc acccgcggcg gagggcgcct
      841 ccctccagtc ccgacgggct cgcccgtcca ccccctccct tgatcctttc ggcggcacag
      901 cgagagcccc cgccgatggg acgagggctc tcctggatca ggccgcgatg gcgtcggcga
      961 gcggctcagc ccccggttcc gcctcgggat cagccgaact gtcggcttca gccgctccag
     1021 cgtcctcctc ggtcggcgcc tcgtcgtcgg tctcgtcgag cgccgctggg cgatcccagg
     1081 acagcaccgc cggcgcccac tgccgttcgg ccgccgcctc ggcgacaaac gtcaccaggg
     1141 cgtccttctt gagggtcttg gcgcggtcgt cttccacctc aagctcgtcc agcatcacca
     1201 gcagttgggc cttggaatgg accgccagat aggcctcatc cggcgtccag tgggcggaga
     1261 tgtccgcgcc gcacagcgcc gcgatctcgg ccgcctcggc ccgcgctcca tggcgcagaa
     1321 ggctggtgcg ggcttcgcgg atgttgagcg acaccgccgt caactccgcc agcaaggcca
     1381 tcttctcccc gtggggcagc gtctcaaccc aggtgatcgg ccgcagcccc gaggcgcgat
     1441 aggcctcgcg tcgggcgtcc agccggccgc gcacctcccc gtccagcgcc gcgatcgggg
     1501 tctgggatgc gcgctgatag cgggtggcgg cgatctgcga cgccgaacca tcgagcgccc
     1561 cgctggaatg cagggccaac tgcttgaaga gctgggcgac caggaccgtc agggcggcgc
     1621 ttggatcatc ggccaagtca cggatcagac cgcgggtggc cacgtcagtg cgggtctcat
     1681 gcaggacgtg actggcgccc tcgacctcca catccgcgcg gggaacctcc acgtcgttgg
     1741 aggccccgcc aaaccnnnnn nnnnnatcct cgatggggag ggggaccgag aagaaggtcc
     1801 ccgccaggcc gtgcccatcc gacggggtga gcagcaccgc gccgattttg ctgcgggtca
     1861 gcaccgcgcc cgccgccacg ccgaaggcgc tgaccagggg cgcaagctgg gcaggcgcct
     1921 cttccgtgga cggatcgaga tcctgcaagg cgctctccag ttgggtgacc cgatagcggg
     1981 cgtccgacaa ggccgccttc tggccctcat ccaggtcacg ctcccagaca tgcgggagac
     2041 ggaagaagcc gtccggtgcg ctgtaaccgg gttcgcggcc gacaaacacc gccagcccct
     2101 cggccttcaa atgatcgacg atcggctgga cgcgctcgcg ccaggcgccc tgcaggacct
     2161 ccggatcgag caggaagtcg ggaaattcgc caaacagatc cgaggcgacc cggccgcccg
     2221 cggccaggta gcgatccatg ccgaccaagg tgaagcggtc gtcgtcgacc gtcgcccggt
     2281 cgtgttcgac cacggcgcgc agctgataat cctggaagta gccgtcgagt gcggtctggg
     2341 cgatctcggc ctgttgcttc ttgtcgggca ggcgggcgaa caggcggacc tgcttcaacg
     2401 tcagccggcc ctgccgcagc gccttcagca ccgtcggatg gacggcggcc agggcctcca
     2461 ggcgcttgat ttccagctca gcgtagccga gcgcggtcga gatcgcctgg gtgtccatct
     2521 tcgccttccg cagcttgccg atggcggtga tgacgtcggc gatgtggacc ggcgcatgct
     2581 cagtgttcgg cagcacgatg gcggcggctt gctgggcagg cgtctcggcc agcaggcagt
     2641 cgatgcggta gtcgtcgtcg atctcgccgc actcgcgcaa caggatcagg ccaaagcgcc
     2701 ggcgacggcc atcgagcgcc atgaacaggg cctcgccctt gcgccccgga cgcacgatcg
     2761 gcgggatgac cacgccggcg gccagcaggg tgtcggccag ctgcggcacg ccgtcatcgg
     2821 ccggctcatg gaagcgaagg ttttccttgg cgaggccaag gtcgccgagg cgcacctgga
     2881 tgcggctcat ggcgaccggc gaagtggggg attgatcggt catcggggtc tagctcctcg
     2941 cgcctgggcg agggacatcc tgccc
//
LOCUS       DAOMEO010000312         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3690_length_2960_cov_1.971773, whole genome shotgun sequence.
ACCESSION   DAOMEO010000312 DAOMEO010000000
VERSION     DAOMEO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3690_length_2960_cov_1.971773"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1151)
                     /locus_tag="PLH31_08060"
     CDS             complement(102..1151)
                     /locus_tag="PLH31_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HQR89204.1"
                     /translation="MKTARGFFKPLAIGAPAPYRDLPARVERMIHFVPPHLDKVRAKV
                     PEIAPTVDVILANLEDAIPADAKGAALAGLIEMARTIDFKALGVGFWTRINCLNSPWH
                     LDEVSTIVEKAGDKIDVIMVPKVEGPWDIFYMDQLLASLEAKHGVTRPILLHAILETA
                     EGVMNVEAIAAASPRMQGISLGPADLAASRAMKTTRVGGGHPGYRVIEDPHADGSARA
                     SVQQDLWHYTFAKMVDACAAHGIKPFYGPFGAIDDPVACEQQFRNAFLMGCAGAWSLH
                     PSQIAIAKQVFSPAPDEVIFAKRILEAMPDGTGVAMLDGKMQDDATWKQAKVMVDCAR
                     QIAGKDAEYAKLYGF"
     gene            complement(1304..2332)
                     /locus_tag="PLH31_08065"
     CDS             complement(1304..2332)
                     /locus_tag="PLH31_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR89205.1"
                     /translation="MVENPLERLDTFELVKLGKASVTVGGLAAALMIIIVALFAARLT
                     SAGLQRLRARVAGSAASLYIVEKVLTYGMVIIGFVAGLSTMGLDLTSLAVFAGALGVG
                     VGLGLQGMVKDFASGISLVFERLVAVGDFVELPSGHRGVVHEVGPRATRIRTNDSTDV
                     IVPNSVLVNDLVINWTLRNTNRRIRVPFVTAFGVDKQKVREAVLKAARSVPFTSPDDA
                     VRRTQVWLVGYGEHALKFELIVWPTVEAVRRPAAIFAAYTWAIDDALREAGVEIPYPQ
                     REVRVRGLFGEEGEDALTSLKLEPAARRRGKAKAAKPGSSNDAARDLEREDPDDPPMP
                     PPAPAPKG"
     gene            complement(2366..>2960)
                     /locus_tag="PLH31_08070"
     CDS             complement(2366..>2960)
                     /locus_tag="PLH31_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQR89206.1"
                     /translation="CVIAGSLLQTLIVHGGIVRLMAWLPVVPFFRGIIDAMLVGFSTA
                     SSSASLPVAIRVAEDNLGIKPPVTSTVLPLGATIGMDGTAMYVAMLTLFAVQAFGVQL
                     EPAQYVLIAVTTTIVAMGVAPVPSGSLFILAAVLGSIGIGPEQTALIVGFILPFDRVL
                     DMIRTVPNVTADLAVATAVGRWEKEIDVEVYKGCPPS"
BASE COUNT          496 a          993 c         1012 g          459 t
ORIGIN      
        1 ggaggtggcc cagagggccg gagggggcca gctcggctta ggccgcattg gccccctcag
       61 tcgctccgcg acagctcccc cagaggggga gcagctaccg atcagaaccc atacagcttg
      121 gcatactccg catccttgcc cgcgatctgc cgcgcgcagt cgaccatcac cttggcctgc
      181 ttccaggtcg cgtcgtcctg catcttgccg tccagcatgg cgacgcccgt gccgtccggc
      241 atggcttcca ggatgcgctt ggcgaagatc acctcgtcgg gagccgggct gaacacttgc
      301 ttggcgatgg cgatctggct ggggtgcagg ctccaggcgc cggcgcagcc catcaggaag
      361 gcgttgcgga actgctgctc gcaggcgacc ggatcatcga tcgcgccgaa cgggccatag
      421 aacggcttga tgccatgggc ggcgcaggcg tcgaccatct tggcgaaggt atagtgccac
      481 aggtcctgct gcaccgaggc gcgggccgag ccgtcggcgt gcgggtcctc gatgacgcga
      541 tagccggggt gaccgccgcc cacgcgagtg gtcttcatgg cgcggctggc ggcgagatcc
      601 gccgggccca gtgaaatgcc ctgcatccgc gggctggcgg cggcgatggc ctcgacattc
      661 atcacgccct cggccgtttc caggatggcg tgcagcagga tgggccgggt gacgccgtgc
      721 ttggcctcca gcgaggccag cagctggtcc atatagaaga tgtcccatgg cccctcgacc
      781 ttaggcacca tgatcacgtc gatcttgtcg ccggccttct cgacgatggt cgagacctca
      841 tccaggtgcc agggcgagtt caggcagttg atccgggtcc agaagccaac gcccagggcc
      901 ttgaagtcga tggtccgggc catctcgatc agaccggcca gggccgcgcc cttggcgtcg
      961 gcggggatgg cgtcttcgag attggccagg atcacatcga cggtcggcgc gatctccgga
     1021 accttggcgc gcaccttgtc cagatgcggc ggcacgaagt ggatcatgcg ctccacgcgg
     1081 gcgggcaggt cgcgataggg cgcaggcgcg ccgatggcca agggtttgaa aaagcctcgc
     1141 gcggtcttca ttggtcgctc cccgtcattg tctggcgcgt gacgggcgcc gaaaccggcg
     1201 tccgctttcg actgtcacgc ccgctgttct tttcgcagtt gcgagaagat tgcggcggcg
     1261 cggcagagcc gtcaagggct cagcttatca gcgttcagaa acctcagccc ttgggcgctg
     1321 gcgcgggcgg cggcatgggc gggtcatcgg gatcctcgcg ctccaggtcg cgggcggcat
     1381 cattgctgga gccgggcttg gcggccttgg ccttgccccg gcggcgggcg gcgggttcca
     1441 gcttgaggct ggtcagggcg tcctcgccct cttcgccgaa caggccgcgc acgcgcacct
     1501 cgcgctgcgg atagggaatt tcaacgcccg cctcgcgcag ggcgtcgtcg atggcccagg
     1561 tataggccgc gaagatcgcc gccgggcggc gcaccgcctc gaccgtgggc cagacgatca
     1621 gctcgaactt cagggcgtgc tctccatagc cgaccagcca cacctgggtg cggcgcacgg
     1681 cgtcgtcggg cgaggtgaag ggcacgctcc tggccgcctt caagaccgcc tcgcgcacct
     1741 tctgcttgtc gacgccgaag gcggtgacga agggcacccg gatgcggcgg ttggtgttgc
     1801 gcagggtcca gttgatgacc aggtcattga ccagcaccga attgggcacg atcacgtcgg
     1861 tgctgtcatt ggtgcgaatg cgggtggcgc ggggccctac ctcatgcacc acgccgcgat
     1921 ggccgctggg caattcgacg aaatcgccca ccgccaccag ccgttcgaac accagcgaaa
     1981 tgcccgaggc gaagtccttg accatgccct gcaggcccag acccacgccc acgcccaggg
     2041 cgccggcgaa cactgccagc gaggtcaggt ccagacccat ggtcgacagg ccggccacga
     2101 acccgatgat gaccatgccg taggtcagca ccttctcgac gatgtacagc gaggcggcgc
     2161 tgccggccac ccgggccctc aggcgctgca agccggccga ggtcaggcgg gcggcgaaca
     2221 gggccacgat gatgatcatc agggccgcag ccaggccgcc gaccgtcacc gaggccttgc
     2281 ccagcttgac cagctcgaag gtgtccaggc gttccagagg attctcgacc atgggcggca
     2341 ctgtgtgccg cgccgcgcgg cgggatcaag agggcgggca ccctttgtag acctcgacgt
     2401 cgatctcttt ctcccagcgc cccacggcgg tcgccacggc caggtccgcc gtgacattgg
     2461 gcacggtgcg gatcatgtcc agcacccggt caaacggcag gatgaagccg acgatcaggg
     2521 cggtctgctc gggcccgatg ccgatcgagc ccaatacggc ggccaggatg aacagcgagc
     2581 ccgacggcac cggcgccacg cccatggcga cgatggtggt ggtcacggcg atcagcacat
     2641 actgggccgg ctccagttgc acgccaaagg cctgaaccgc gaacagggtc agcatggcca
     2701 cgtacatcgc cgtgccgtcc atgccgatgg tcgcgcccag cggcaggacg gtggaggtga
     2761 ccggcggctt gatgcccaga ttatcctcgg ccacccggat cgcgaccggc aggctggccg
     2821 agctggacgc ggtggaaaaa cccaccagca tggcgtcgat gatccccctg aagaacggga
     2881 ccaccggcag ccaggccatc agccgtacaa tacccccatg cacgatcagg gtctggagca
     2941 gcgagccggc gatcacgcac
//
LOCUS       DAOMEO010000313         2954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3699_length_2954_cov_2.567782, whole genome shotgun sequence.
ACCESSION   DAOMEO010000313 DAOMEO010000000
VERSION     DAOMEO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_2954_cov_2.567782"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLH31_08075"
     CDS             <1..261
                     /locus_tag="PLH31_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR89207.1"
                     /translation="KTYTAFGRALSGIDVIRAIKIGEPVPDPQDRMISVRLLADLPAD
                     KRPSVQVMDTRSPAFAALVAKRRTEVGPSFTNCDVEIPVQIK"
     gene            258..1124
                     /locus_tag="PLH31_08080"
     CDS             258..1124
                     /locus_tag="PLH31_08080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR89208.1"
                     /translation="MKPAAVVVLGLSLALLAEPALSAPKSRAKARGVTAPAAADWRVP
                     DPDNVMVIDTNRGRVIVELIPEVAPGHVTRMRELTKAGVYDGRTFFRVIDRFMAQTGD
                     PKDNGEGQSDKPNLKAEFTFRRTAATPFAPAAAPSGLQVGLLGSLPVVSQHWSWSAVT
                     ADGNVSAWGTYCPGVVGMARDDDNNSANSQFFLMRQPYPSLDKRYTAFGRVISGLETI
                     RAIKTGEPVAAPQDRMEKVRMLADIPETERPKVRVINAQGAWFAGEIRRQRAIKGADF
                     SVCDLKLPVDIR"
     gene            1215..1675
                     /locus_tag="PLH31_08085"
                     /pseudo
     CDS             1215..1675
                     /locus_tag="PLH31_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            1866..>2954
                     /locus_tag="PLH31_08090"
     CDS             1866..>2954
                     /locus_tag="PLH31_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HQR89209.1"
                     /translation="MLLVLLAAALHAPFAMAAPPSTSTPAPAKSRLIVLSDIENEPDD
                     TQSFVRLLLYANDIDIEAMVATTSTHMRDQIHPESIRRLVSIYGQVRSNLLLHAPGYP
                     TVKALEARIAAGQPGYGLSATGPGRDTEGSRRIIAALEASDPRPVWVSVWGGANTLAQ
                     ALQTLKASRSREEVARLTAKLRVYTISDQDDAGAWIRKTFPDIFYIVSPGGYGAATWG
                     GVNNPVDGLDNTSVSNAWLAQHIQQGHGPLGAAYPDVAYSMEGDTPAFLGLIPNGLSE
                     PEHPDWGGWGGRYELYTPALEALDPKGFNGGVPIETETRPIWTNAVDTVTPREPAAHG
                     RPFQSGKRAYKDYRATIWRWRDAFQNDFA"
BASE COUNT          521 a          991 c          946 g          496 t
ORIGIN      
        1 aagacctaca ccgcctttgg ccgggccctg tcggggatcg acgtgatccg cgcgatcaag
       61 atcggcgagc ccgtgcccga tccgcaggac aggatgatca gtgtacggct tctggccgac
      121 ctgccggccg acaagcgccc ttcggtccag gtgatggaca cccgcagccc cgccttcgcc
      181 gccctggtgg ccaagcgccg gaccgaggtg ggccccagct tcaccaattg cgacgtcgaa
      241 atcccggtgc agatcaaatg aagccagccg ccgttgtcgt cctgggtctg agcctggccc
      301 ttctggcgga gccggcgctt tccgccccca agtccagggc caaggcgcgg ggcgttaccg
      361 cgcccgctgc ggcggactgg cgggtccccg accccgacaa cgtgatggtg atcgacacca
      421 acaggggccg ggtgatcgtc gagctgatcc ccgaggtcgc gcccggtcat gtgacgcgga
      481 tgcgcgaact gaccaaggcc ggcgtctatg acggccgcac cttctttcgg gtcatcgacc
      541 gcttcatggc ccagaccggc gatcccaagg acaatggcga agggcagtcg gacaagccca
      601 acctgaaggc cgagttcacc ttccggcgca ccgcggcgac gcctttcgcg cctgcggcgg
      661 cgccgtcggg gttgcaggtc gggctactgg gctccctgcc ggtggtcagt cagcactgga
      721 gctggagcgc cgtcaccgcc gacggcaatg tctcggcctg gggaacctac tgcccgggcg
      781 tggtgggcat ggcgcgcgac gatgacaaca attcggccaa cagccagttt ttcctgatgc
      841 gccagcccta tccgtcgctg gacaagcgct ataccgcctt tggccgcgtg atcagcgggt
      901 tggagacgat ccgcgccatc aagaccggcg agcccgtggc cgccccgcag gaccggatgg
      961 aaaaggtccg gatgcttgcg gatattccgg agactgagcg cccgaaggtc cgcgtgatca
     1021 atgctcaagg cgcgtggttc gccggcgaga tccgccgcca gcgcgcgatc aagggggccg
     1081 acttctcggt ctgcgacctg aagctgccgg tcgatatccg ttaggttcgg cgcttttcaa
     1141 gcccagccac ggttcacagc gagggtgaaa gcgcgctaga agcgccttca cctttcgaca
     1201 tccaagggat gaccatgtcg gccgacctcg aaaacacctt gatcctcacg ctggaaagcg
     1261 gcccggtcac catccagatg cgtcctggcc tggccacgtc gagcgcatca aggaactggt
     1321 ccgcgagggc ttctatgacg gcgtggtctt ccaccgcgtg atcccgggct tcatggccca
     1381 gggcggcgat ccgtcgggca ccggccgcgg cggcagcgac aagcccgaca tcaaggccga
     1441 gttctccaag gaagcccacg tgcgcggcgt ctgctcgatg gcccgcaccc ccgatcccaa
     1501 ctcggccaac agccagttct tcatctgcct ggacgacgcg accttcctcg acggccagta
     1561 cacggtctgg ggccaggtgt cggaaggcat ggaccacgtc gacgccctgc ccaagggcga
     1621 gcccccgcgc gagccgggca agatcctcag cgccaagatc gccgctgacg cctgatctgg
     1681 cttgaagctg attataaaga cggccccgga gcgatccggg gccgtttttt gttggggctc
     1741 cgctgcgtcg aaatcagctc ccgagcccac tgacgttgcc gagcgaacac gcagtgcttg
     1801 cgtgagcgcc aataggctgg ataccgtggt agcgctaacg tgcggagcca gagatgacgt
     1861 ttcggatgct cttggttctc ctggcggcgg cgcttcatgc gccgttcgcg atggccgcgc
     1921 ccccttcaac ctcgacgccg gcgcccgcga agtcacgcct gatcgtcctt agcgacatcg
     1981 agaacgagcc cgacgatacg cagtccttcg tgagactgct tctgtacgcc aacgatatcg
     2041 acatcgaggc gatggtcgcc acgacctcca cccacatgcg cgatcagatc catccggagt
     2101 cgatccgacg gctggtgtcg atctacggcc aggtcagatc caatctgctg cttcacgcac
     2161 cgggttatcc gacggtgaag gccttggagg cccggatcgc ggccggccag cctggctatg
     2221 gtctgtcggc cacggggccg ggcagggaca ccgagggatc gcgccggatc atcgcagcgc
     2281 tggaagcctc cgatccccgt ccggtctggg tgtcggtctg gggcggcgcc aacaccctgg
     2341 cccaggctct gcaaaccttg aaagcgtcgc gctcgcggga agaggtggcg cggctgaccg
     2401 ccaagctgcg cgtctacacg atctcggatc aggacgacgc tggagcctgg atccgcaaga
     2461 cgttccccga cattttctat atcgtcagtc ccggtggtta tggggcggcc acctggggcg
     2521 gagtaaacaa tccggtcgac ggtctcgaca acacaagcgt ctccaacgcc tggctggctc
     2581 aacacatcca acagggtcat ggacccttgg gcgcggccta tcccgacgtc gcctacagca
     2641 tggaaggcga tacgccggcg ttcctgggcc tgattcccaa tggcctgtcg gagcctgaac
     2701 atcctgactg gggcggctgg ggtggtcgct atgagctcta cacgcccgcc ttggaggcgt
     2761 tggatccaaa aggcttcaac ggcggcgtgc cgattgagac cgagacccgg cctatctgga
     2821 ccaacgcagt cgatacggtg accccgcgcg agcccgcagc tcacggccgg ccctttcaga
     2881 gcggaaaacg cgcttacaag gattatcggg ccacgatctg gcgttggcgc gacgccttcc
     2941 agaacgactt cgcc
//
LOCUS       DAOMEO010000314         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3716_length_2937_cov_2.834490, whole genome shotgun sequence.
ACCESSION   DAOMEO010000314 DAOMEO010000000
VERSION     DAOMEO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3716_length_2937_cov_2.834490"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLH31_08095"
     CDS             complement(<1..443)
                     /locus_tag="PLH31_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HQR89210.1"
                     /translation="MNPLRHVVRGLAVAATAVALAGCVSLFPKTDPAALYRFGNDPVA
                     PVSKGPAGALFGVFKTPTVFTRAAAGDRILTFTKGEAAYIAGARWVSPASVLFDEAVS
                     RAFEADPGRARLIGRGEVAKSDLMLRLEVRSFETIYTDGPKAAPEV"
     gene            complement(440..1378)
                     /locus_tag="PLH31_08100"
     CDS             complement(440..1378)
                     /locus_tag="PLH31_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQR89211.1"
                     /translation="MERNANYALVGAVTLLLFMGLVVFVVWLAKLSFNQEYDQYDILF
                     VGPVRGLSEGGEVHFNGIKVGEVSKIALDKSDPNRVIARARVTSDVPIKTDSYATLEP
                     QGITGVNYVQITAGTPDKKLLKDTVENGRVPVIRSQRSALSDLLEGGGTVLTRTIEAL
                     DRVNRVLSDDNIQSFSASLSDVQSVTAELRERKAIIADAQKALQSIDATAAEIAALSA
                     STRGVVEGDGRRSIKELADAAGEIKAAAKDARGMISRLEGPTTDFATTGLPQLSQAIV
                     TLQSAAESLDRLVGQVEQNPRGLVSKAPAKELEVKP"
     gene            complement(1378..2220)
                     /locus_tag="PLH31_08105"
     CDS             complement(1378..2220)
                     /locus_tag="PLH31_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89212.1"
                     /translation="MTVATQPSEDSQEADDEARPILIEGLVSRFGETVVHNGLDLAVQ
                     RGEVLGVVGGSGSGKSVLLNSIIGLKTPDEGTVKLFGHDMRTADRRRWSSVERRWGVL
                     FQQGALFSNLTVRENVAAPLFEHTRLPKREIEELADLKIALSGLPARAAGLKPAELSG
                     GMRKRAGLARALALDPELLFLDEPTAGLDPIGAAAFDDLIKDLSDSLDLTVFMITHDL
                     DSLYAITDRVAVIADKKVVALAPVRELEHSDHPWIKEYFLGPRGRSAGSKAAPAQTTP
                     TRTS"
     gene            complement(2217..>2937)
                     /locus_tag="PLH31_08110"
     CDS             complement(2217..>2937)
                     /locus_tag="PLH31_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR89213.1"
                     /translation="GHLLTAIGRCAADPRRIRWAPIISLAERAGLDAIPIVATTTFFI
                     GAVVALLGANMLIDFGAQVYSVELIGVSVLREFNILITAILLAGRSASSFAAEIGSMK
                     MNQEIDAMQVMGVDPYEALVLPRFAALLLTIPLLTFIATLAGLAGGMLVVWVVLDLSP
                     GFFLQRIVENVGATHFWIGMSKAPVMAMVIAAIGCRQGMEVGNDVESLGRRVTAAVVH
                     AIFAIILIDAVFALLYMKLDI"
BASE COUNT          532 a          943 c          974 g          488 t
ORIGIN      
        1 acctcgggcg ccgccttggg tccatcggta tagatggtct cgaaggaacg aacctctagc
       61 ctgagcataa gatcggactt ggcgacctcg ccgcgaccga tcagtcgcgc acggcccgga
      121 tcggcctcaa aggcgcgtga gacagcttca tcgaacagca cgctggccgg cgacacccag
      181 cgtgcgccgg cgatataggc cgcctcgccc ttggtgaagg tcaggatgcg atcaccggct
      241 gcggcgcgcg taaagaccgt cggggtcttg aacaccccga acagggcgcc ggccggcccc
      301 ttggaaaccg gcgcgacggg atcgttgccg aaccggtaca gcgcggcggg gtcggtcttg
      361 ggaaacagac tgacgcagcc ggcgagggcg actgccgtcg cagcgacggc gagaccgcgg
      421 acaacatggc gtaacgggtt cacggcttga cctccagttc cttggcgggc gccttgctga
      481 ccaggcctcg cggattttgt tcgacctggc cgaccaggcg atccagggac tcggccgccg
      541 actgcagggt gacgatggcc tgcgaaagct ggggcaggcc ggtggtggca aagtcggtgg
      601 tcggaccctc cagccgggag atcatgccgc gcgcgtcctt ggcggccgcc ttgatctctc
      661 cggcggcgtc ggccagttcc ttgatcgacc gccgtccatc gccctcgacg acgccgcggg
      721 tggacgcgga cagcgcggcg atttcggcgg cggtcgcgtc gatgctctgc agggccttct
      781 gagcgtcggc gatgatcgcc ttgcgctcac gcagctcggc ggtcacggac tgaacgtcgc
      841 tcagcgacgc gctgaagctc tggatgttgt cgtccgagag cacgcgattg acccggtcca
      901 gcgcctcgat ggtgcgggtc agcaccgttc caccgccctc cagcaggtca gaaagagcgc
      961 tacgctggct gcggatcaca ggcacccggc cgttctcgac agtgtccttc aggagcttct
     1021 tgtcgggcgt accggcggtg atctggacat agttcacgcc ggtgatgcct tgcggctcca
     1081 gcgtggcgta gctgtcggtc ttgatgggca catcagaggt gacgcgcgca cgggcgatga
     1141 cgcgattggg gtcactcttg tcgagcgcaa tcttgctgac ctcgccgacc ttgatgccat
     1201 tgaaatgcac ctcgccgccc tccgagagcc cgcgcacggg gcccacgaac aggatgtcgt
     1261 actggtcata ctcctgattg aagctcagct tggccagcca gaccacgaaa accaccagcc
     1321 ccatgaacag gagcagggtc accgcgccga ccagggcgta gttggcgttt ctttccatca
     1381 gctggtcctc gtcggggtcg tctgggctgg agccgccttg ctgcctgcag agcggccgcg
     1441 cggtcccagg aaatattctt tgatccaggg atgatcggag tgttcgagct cccgcaccgg
     1501 cgcgagcgcg accaccttct tgtcggcgat cacggcgacc ctgtcggtga tcgcgtaaag
     1561 gctgtccagg tcgtgggtga tcatgaacac ggtcagatca aggctgtcgg acaggtcctt
     1621 gatcaggtcg tcaaaggccg ccgcgccgat cgggtccagg cccgccgtcg gctcgtccag
     1681 gaacagaagc tccggatcca gggccagggc gcgcgccagg ccggcgcgct tgcgcatacc
     1741 gcccgagagc tcggcgggct tgagaccggc ggcgcgagcg ggcaggcccg aaagggcgat
     1801 cttcagatcc gccagttcct cgatctcgcg tttgggcagc cgggtgtgct cgaacagcgg
     1861 cgcagcgaca ttctcgcgca cggtcaggtt cgaaaagagc gccccttgct ggaatagcac
     1921 gccccagcgc cgctccacag agctccagcg gcggcgatcg gcggtgcgca tgtcgtggcc
     1981 aaacagcttg accgtaccct catcgggggt cttcagacca ataatgctgt tgagcagcac
     2041 cgacttgcct gatcctgacc cgccgaccac gcccagcact tcgccgcgct gaaccgccag
     2101 gtcgaggccg ttatgaacga ccgtctcgcc aaagcgcgac actaggccct cgatcaggat
     2161 cggccgagct tcatcatccg cttcttgaga atcttcagag ggttgagtgg cgacggtcat
     2221 atgtcgagct tcatatacag cagggcgaag accgcatcga tcaggatgat ggcgaaaatg
     2281 gcgtggacga cggcggcggt cacgcgacga cccagggatt ccacgtcgtt gccgacctcc
     2341 atgccctgtc ggcagccaat cgcggcgatc accatggcca tgacgggcgc cttggacatg
     2401 ccgatccaga aatgggtggc ccccacgttt tccacaatgc gttgcaggaa gaagccgggc
     2461 gacaggtcca gcaccaccca caccaccagc atgccgccgg ccaggcccgc cagtgtggcg
     2521 atgaaggtca gcagcggtat ggtcagtaac agggcggcga accgcggcag gacaagggct
     2581 tcgtaaggat cgacccccat gacctgcatg gcgtcgatct cctggttcat cttcatcgaa
     2641 ccaatttcgg cggcgaagct ggaggccgag cggccggcca gcaggatggc ggtgatcagg
     2701 atgttgaact cgcgcagcac agagacgccg atcagttcca ccgaatagac ctgggcgccg
     2761 aagtcgatga gcatattggc gccgagcagg gccacgaccg cgccgatgaa gaaggtggtg
     2821 gtcgcgacga tgggtatcgc gtccaggccg gcgcgttccg ccagcgaaat gatcggcgcc
     2881 caccttatgc ggcgcggatc cgccgcgcag cgaccgatgg cggtcagcag atggccc
//
LOCUS       DAOMEO010000315         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3723_length_2933_cov_2.416261, whole genome shotgun sequence.
ACCESSION   DAOMEO010000315 DAOMEO010000000
VERSION     DAOMEO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3723_length_2933_cov_2.416261"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLH31_08115"
     CDS             <1..935
                     /locus_tag="PLH31_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HQR89214.1"
                     /translation="YASNLRSASHRGYDRPPGGGINGAPIPSALYGLHDVLDPQVWSS
                     MAFFMGHEMQTTMFQPVGGMDMIGKAFARQVEGQITYNAKVSKIAQDDKGVAVTYTDT
                     VTGKVVTAQADWCVCTIPLSVLGQIDTQVTDEMMAAIKAVPYSGQVKIGLEMKRRFWE
                     EEDSIFGGHSFTDQEIALISYPNNNLFGDGPAVLLGAFAREMGAYRLAGLTPQQRIEV
                     ALQQGSVLHPASYRKEFSNGASVAWSRVPWSLGCCARWTEQSRKEHYQTLVGMDRRIV
                     LAGEHASYVGCWMEGALLSSIDAITRLHKRALEA"
     assembly_gap    1115..1124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1125..1380
                     /locus_tag="PLH31_08120"
     CDS             <1125..1380
                     /locus_tag="PLH31_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR89215.1"
                     /translation="AKGGVGAGTIPALASNPRLAGAAYPISAIVLGKGGMPGLTGSLD
                     DTQIAEVVTYIRTNFGNAYAKPVTAAEVARIPHPTRGGGH"
     gene            1469..>2933
                     /locus_tag="PLH31_08125"
     CDS             1469..>2933
                     /locus_tag="PLH31_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR89216.1"
                     /translation="MALPDEDRYRLLVDAIIDYAIYMLDIDGRVTSWNPGAQRFKGYT
                     PDEIIGQHFSRFYTPEDQATGDPATALGVAAREGRFEKEGWRQRKDGSRFWAHVVIDA
                     IRDEAGALMGFAKITRDITERMETQKALDQAREALFQSQKMEAIGQLTGGVAHDFNNL
                     LMAILGSLELVRKRVPYDPRITPLLDNAMQGAERGSILTQRMLAFARKQELKMDAVDV
                     ASLARGMMTFLDRTIGGKIEVSLRFPADLPRVLTDPYQLEAALMNLAVNARDAMPDGG
                     DLIISGSAEQVEGRPDLAPGHYVRLSVTDTGEGMSPETVSRATDPFFTTKGVGKGTGL
                     GLSMVHGLAAQSGGGLFLTSKAGAGTQVDLYLPVVRKDCGAAARPDAVAATGSAGRKL
                     TILAVDDDPLVLTNTTAMLDDLGHHVIAVNTGKDALEILRSRRDIDLIVSDELMPHMS
                     GSQLAKAVQRSHPGMPFIIATGYADLTTVAGDDTRLGL"
BASE COUNT          496 a         1002 c          979 g          446 t
ORIGIN      
        1 ggtacgcctc caacctgcgc tcggcctcgc accggggcta tgaccgtccg ccgggcggcg
       61 gcatcaacgg cgcgccgatc ccgtccgccc tctacggcct gcacgacgtg ctggacccgc
      121 aggtctggag ctcgatggcc ttcttcatgg gtcacgagat gcagaccacc atgttccagc
      181 cggtgggcgg catggacatg atcggcaagg ccttcgccag gcaggtcgag ggtcagatca
      241 cctacaatgc caaggtcagc aagatcgccc aggacgacaa gggcgtggcc gtcacctata
      301 ccgacaccgt gacgggcaag gtcgtgaccg cccaggccga ttggtgcgtc tgcaccattc
      361 cgctgtcggt gctgggccag atcgacaccc aggtcaccga cgagatgatg gccgccatca
      421 aggccgtgcc ctattcgggc caggtcaaga tcggcctgga gatgaagcgc cgcttctggg
      481 aggaggaaga ctccatcttc ggcggtcaca gcttcaccga ccaggagatc gccctgatct
      541 cctatcccaa caacaatctg ttcggcgacg ggccggcggt gctgctgggg gccttcgccc
      601 gcgagatggg ggcctatcgc ctggcgggct tgacgccgca gcagcgcatc gaggtcgccc
      661 tccagcaggg ctcggtcctg cacccggcca gctatcgcaa ggagttcagc aacggcgcgt
      721 ccgtggcctg gagccgggtg ccctggtcgt tgggctgctg cgcccgctgg accgagcaat
      781 cccgcaagga acactaccag accctggtgg gcatggaccg ccgcatcgtc ctggccggcg
      841 agcacgcctc ctatgtcggc tgctggatgg agggggcgct gctgtcctcg atcgacgcca
      901 ttacccgcct gcacaagcgc gccctggagg cctgagcatg accatccgct tcccgattgc
      961 cctggccgcc gcagcgctcg ccatcaccgc gcccgccgtc gcccagaccc cgcccggcgg
     1021 cgacaccggc ggcggcattg tccgcatcgc caagccggtg acgggcaagg aggtctatga
     1081 ggctgtctgc caggcctgcc acatggccga cgccnnnnnn nnnncgccaa gggcggggtg
     1141 ggagcgggga ccattccggc cctggcgtcc aatccgcgtc tggcgggagc ggcctatccg
     1201 atctcggcca tcgtactggg caaggggggc atgccgggcc tgaccggctc gctcgacgac
     1261 acccagatcg ccgaagtggt gacctatatc cgcaccaatt tcggcaatgc ctatgccaag
     1321 ccggtgacag cggccgaggt cgccaggatc ccgcatccga cccggggcgg cgggcactga
     1381 gcgaggcgga accacccggc tgaccgcgcg ttccggacct ctggtgacga gccggggacg
     1441 ggttttggaa cacgggaaga acttggcttt ggcgcttcca gacgaggatc ggtatcggct
     1501 gctggtcgat gcgatcatcg actacgccat ctacatgctc gatatcgacg gccgggtcac
     1561 cagctggaat cccggcgccc agcggttcaa gggctatacg cccgacgaga tcattggcca
     1621 gcatttctcg cgcttctaca cccccgaaga ccaggcgacg ggcgaccccg ccacggccct
     1681 cggcgtggcc gcccgtgagg gccggttcga gaaggagggc tggcgccagc gcaaggacgg
     1741 cagccgattc tgggcgcatg tcgtgatcga cgccatccgg gacgaggccg gggccctgat
     1801 ggggttcgcc aagatcaccc gcgacatcac cgagcgcatg gagacccaga aggcgctgga
     1861 ccaggcccgc gaggcgctgt tccagtcgca gaagatggag gcgatcggcc agctgaccgg
     1921 cggcgtggcg cacgacttca acaacctgct gatggccatc cttggcagcc tggagctggt
     1981 gcgaaagcgc gttccctacg atccgcgcat cacgccgttg ctggacaacg ccatgcaggg
     2041 cgccgagcgt ggctcgatcc tgacccagcg catgctcgcc ttcgcgcgca agcaggaact
     2101 gaagatggac gccgtggacg tggccagcct ggcccgcggc atgatgacct ttctggatcg
     2161 caccatcggc ggcaagatcg aggtcagtct gcgcttcccc gccgatctgc cccgggtgct
     2221 gaccgatcct taccagctgg aggcggcgct gatgaacctg gcggtcaatg cgcgcgacgc
     2281 catgcccgac ggcggtgacc tgatcatcag cggcagtgcc gaacaggtcg aaggccgccc
     2341 cgatctggcc cccggccact atgttcgcct cagcgtgacc gatacgggtg agggcatgag
     2401 cccggaaacc gtttcgcgcg ccaccgatcc gttcttcacc accaagggcg tgggcaaggg
     2461 cacgggcctt ggcctgtcca tggtgcacgg cctggccgcc cagtcgggcg gcggcctgtt
     2521 cctgaccagc aaggccggcg ccggcaccca ggtggatctg taccttcccg tcgtgcggaa
     2581 ggactgcggc gcggccgcca ggccagacgc cgtcgcggcg acgggttcgg ccgggcgcaa
     2641 gctgaccatc ctggcggtgg acgacgaccc cctggtcctg accaacacca cggcgatgct
     2701 ggacgatctg ggtcatcacg tgatcgcggt gaatacgggc aaggacgcgc tggagatcct
     2761 gcgcagccgc cgcgacatcg acctgatcgt cagcgacgag ctgatgccgc acatgagcgg
     2821 ctcgcagctg gcaaaggccg tccagcgcag ccatcctggc atgccgttca tcatcgccac
     2881 gggctatgcc gatctgacga cggtcgcagg cgacgatacc cggctgggcc tgc
//
LOCUS       DAOMEO010000316         2917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3740_length_2917_cov_2.742837, whole genome shotgun sequence.
ACCESSION   DAOMEO010000316 DAOMEO010000000
VERSION     DAOMEO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_2917_cov_2.742837"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLH31_08130"
     CDS             complement(<1..540)
                     /locus_tag="PLH31_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89217.1"
                     /translation="MLQIKDLTFNAWGRKFFDHASVSLPPGAKVGLIGRNGVGKSTLF
                     KLILGQLAASEDEISLPKSARIGSVDQEHPATPVSLLDTVLEADEERHSLLTRLEDAD
                     PEDMGEIWARLIEIDADAAPAKASEILSGLGFSQEDLQRPMAEFSGGWRMRVALAAAL
                     FAEPDMLLLDEPTNYLDLEG"
     gene            637..1056
                     /gene="ndk"
                     /locus_tag="PLH31_08135"
     CDS             637..1056
                     /gene="ndk"
                     /locus_tag="PLH31_08135"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQR89218.1"
                     /translation="MTERTFSIIKPDATRRNLTGAINAVIEAAGLRIVAQRRVKLTEA
                     QAKKFYEVHAERPFYGELVGQMIAEPVVVQVLEAEGAVAKYRDVMGATNPKDAAEGTI
                     RKLFALSIGENSVHGSDSLENAAIEIAQFFKDEDIVG"
     gene            1221..2294
                     /locus_tag="PLH31_08140"
     CDS             1221..2294
                     /locus_tag="PLH31_08140"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQR89219.1"
                     /translation="MSENAEQIDQLLNNPDLASVLESDGFRQFLDQAPIAIAVSELHP
                     VERIVYANIEFERLSGQTGADLEGQSWRALLGRAEGAEDDGALSDAVVDHQDYIGVFS
                     FTRSQGVEAVDAWSNIIQDNAGAPVFRMLALVGATSRDADRSEFEQRILDKDVLLREL
                     QHRVANNLQMITALIRIEARNVPEDAKDERFSRLAGRVEALGLLYRSLSEEGQAGTVD
                     LGVYLSEIASAVMRAHAVEGIHLNLQVDTWPVSINVAMPTGLVVNELLTNALKHAFKD
                     REGGTITLHSLVDDEGCRVVVADDGVGLPQGYVWPRLGKLSNMIVQSLRQNANAKVEV
                     GAEPGGGMRVTIFFTRASALSAS"
     gene            complement(2314..>2917)
                     /locus_tag="PLH31_08145"
     CDS             complement(2314..>2917)
                     /locus_tag="PLH31_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQR89220.1"
                     /translation="AQNSLWAGLVLFALTLLEALLLVGLLIPIVGVMLMAGALVAQGA
                     LHPAEAILWCSAGAVAGDALSYLLGRGVGARRLSTGLLAGHRRLFARARLLSRKHGVI
                     AIAAARFMGPVRPFIPIVAGMSRSRPWAFQAASALTAPLWVISLLAPGYLAARNLHLL
                     QTDPAAGAALALGAAILAIAAYAGWKRFGPAPARSARLAV"
BASE COUNT          514 a          965 c          973 g          465 t
ORIGIN      
        1 gccttccagg tcgagatagt tggtcggttc gtccagcagc agcatgtccg gctcggcgaa
       61 cagggcggcc gccagggcca cgcgcatccg ccagccgccg gagaactcgg ccatcggccg
      121 ctgcaggtct tcctggctga agcccaggcc ggacagaatt tccgaggcct tggccggcgc
      181 cgcgtcggcg tcgatctcga tcagccgggc ccagatctcg cccatgtcct cgggatcggc
      241 gtcttccaga cgcgtcagca ggctgtggcg ctcctcgtcg gcctccagca ccgtgtcgag
      301 caggctgacg ggcgtggcgg ggtgttcctg gtccaccgag ccgatgcggg ccgacttggg
      361 caggctgatc tcgtcttcgc tggcggccag ctggcccagg atcagcttga acagcgtcga
      421 cttgcccacg ccattgcggc cgatcaggcc caccttggcg ccgggcggca ggctgacgct
      481 ggcgtggtcg aagaacttcc gtccccaggc gttgaaagtc aggtctttga tctgaagcat
      541 ttgattttga aggtacgcgg cgggttggcg gggagggagc gcccggctat aagcccgcaa
      601 cctcccaatt cacaacttta tgtgtgagat atcttcgtga ccgaacgcac cttctcgatc
      661 atcaagccgg acgccacgcg tcgcaacctg accggcgcca tcaatgcggt gatcgaggcc
      721 gccggccttc gcatcgtcgc ccagcgtcgc gtcaagctga ccgaagccca agccaagaag
      781 ttctacgaag tccacgccga gcgcccgttc tatggcgaac tggtcggcca gatgatcgct
      841 gagccggtcg tcgtgcaggt tctggaagct gaaggcgccg tggccaagta ccgcgacgtg
      901 atgggcgcca ccaacccgaa ggacgccgcc gaaggcacca ttcgcaagct gttcgccctg
      961 tcgatcggcg aaaactcggt ccacggttcg gacagcctgg agaacgccgc gatcgagatc
     1021 gcccagttct tcaaggacga agacatcgtt ggctaaaggc ttttcgagcg gcgagcttcg
     1081 ctgcctcgaa tagtttagag aagcggcgcg gcttatggcc acgccgcttt tcttatgtct
     1141 gcagcgcgca ttgcgcgtat acaggcggtc ttccgctccc ccggcgtcgt ctggagaccg
     1201 aaagggcgga gtacaaagac gtgagcgaaa acgccgagca gatcgatcag ctgctcaaca
     1261 atcccgacct tgccagcgtt ctggagagcg acgggtttcg acagtttctc gatcaggcgc
     1321 cgatcgccat cgccgtctcg gagctgcatc cggtcgagcg catcgtctat gccaatatcg
     1381 agttcgagcg gctaagcggc cagacaggcg ccgaccttga gggacagtcc tggcgggccc
     1441 tgttggggcg cgctgaaggt gcggaggacg atggcgcgct cagcgacgcg gtcgtcgacc
     1501 atcaggacta tatcggcgtc ttctccttca cccgcagcca gggcgtggag gcggtcgacg
     1561 cctggtcaaa catcatccag gacaatgccg gcgcgcccgt attccgcatg ctggccctgg
     1621 tgggcgcgac cagccgcgac gccgatcgca gcgagttcga gcagcgcatc ctggacaagg
     1681 atgtcctgct gcgcgagctg cagcaccgcg tggccaacaa tctgcagatg atcaccgccc
     1741 tgatccgcat cgaggcgcga aacgtgcccg aggacgccaa ggacgaacgg ttcagccgcc
     1801 tggctggtcg ggtcgaggcc ctgggccttc tctatcgctc gctctccgag gagggccagg
     1861 cggggaccgt cgatctgggc gtctatctca gcgagatcgc ctcggccgtg atgcgcgccc
     1921 acgcggtgga ggggatacac ctcaatctcc aggtcgatac gtggccggtt tccatcaatg
     1981 tggcgatgcc gaccggcctg gtcgtcaatg agctgctgac caacgccctc aagcacgcct
     2041 tcaaggaccg cgagggcggg acgatcaccc ttcacagcct ggtcgatgac gaggggtgtc
     2101 gggtggtggt cgccgacgac ggcgtgggcc tgcctcaggg ctatgtctgg cccaggcttg
     2161 gcaagctgag caacatgatc gttcagtccc tgcgccagaa cgccaacgcc aaggtcgagg
     2221 tcggcgccga gcctggcggc gggatgcgag tgacgatctt ctttacccgc gccagcgccc
     2281 tgtcggcgtc ctgacgctgc ggtctggttc tgctcagacg gccaggcgag cggatcgggc
     2341 cggggcgggg ccaaaccgct tccagcccgc ataggccgcg atcgccagga tcgccgcgcc
     2401 cagcgccaag gccgccccgg ccgccggatc agtctgcagg aggtgcaggt tgcgggcggc
     2461 cagatagccg ggcgccagca gtgagatcac ccacagcggc gccgtcaggg cgctggcggc
     2521 ctggaacgcc caggggcgcg agcgcgacat gcccgccacg atcgggatga acggccgcac
     2581 gggacccatg aaccgcgccg cggcgatggc gatcacgcca tgcttgcgcg acagcagacg
     2641 agcccgggcg aacagacgac gatggccggc cagaaggcct gtcgacaggc gccttgcgcc
     2701 cacgcctcgg cccagcagat aggacagggc gtcgcccgcc acggccccgg cgctgcacca
     2761 caggatggcc tctgccggat gcagggcgcc ttgcgccacc agggcgcccg ccatcagcat
     2821 caccccgaca atcgggatca gcagcccgac cagaagcagg gcttccagca gggtcagggc
     2881 gaacagcacc aggcccgccc agaggctgtt ctgggcg
//
LOCUS       DAOMEO010000317         2914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3747_length_2914_cov_2.618748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000317 DAOMEO010000000
VERSION     DAOMEO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3747_length_2914_cov_2.618748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>511)
                     /locus_tag="PLH31_08150"
     CDS             complement(<1..>511)
                     /locus_tag="PLH31_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQR89221.1"
                     /translation="VEIVKATFPRQLSLAGLRIVIDCANGAAYKVAPVTLYELGAEVI
                     PLGVDPDGTNINHECGSTHPEAMAKLVREYRADIGIALDGDADRLVICDENGLVVDGD
                     QIMAIIAAAYAKAGKLKAGGVVATVMSNLGLERFLGEQGLSLQRTSVGDRYVMARMRE
                     GGFNLGGEQS"
     assembly_gap    512..521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<522..1002)
                     /locus_tag="PLH31_08155"
     CDS             complement(<522..1002)
                     /locus_tag="PLH31_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQR89222.1"
                     /translation="MSKRAYFGTDGIRGQANQHPMTAEVALRVGLAAGKLFRSQDDRR
                     HRVVIGKDTRLSGYMIEPALVAGFASVGMDVRLFGPLPTPAVAMMTRSMRADLGVMIS
                     ASHNNFADNGIKLFGPDGYKLSDEQELKIEALMDEGLQEGLAPPRGLGRVKRIDDAQA
                     "
     gene            1162..2914
                     /gene="glmS"
                     /locus_tag="PLH31_08160"
                     /pseudo
     CDS             1162..2914
                     /gene="glmS"
                     /locus_tag="PLH31_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515492.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
BASE COUNT          494 a          996 c          937 g          477 t
ORIGIN      
        1 gactgctccc cgcccaggtt gaagccgccc tcgcgcatcc gcgccatcac atagcggtcg
       61 ccgacgctgg tgcgctgcag gctcagcccc tgttcaccga ggaagcgctc gaggcccaga
      121 ttggacatca ccgtagccac cacgccgccg gccttcagct tgccggcctt ggcataggcg
      181 gcggcgatga tggccatgat ctggtcgcca tcgacgacta ggccgttttc gtcgcagatc
      241 accagccggt cggcgtcgcc atccagggcg atgccgatat cggcgcggta ttcacgcacc
      301 agcttggcca tggcttcggg atgggtcgag ccgcactcgt gattgatatt ggtgccgtcg
      361 ggatcgacgc ccagcggaat gacctcagcg cccagctcgt acagggtgac cggcgccacc
      421 ttgtaggcgg ctccgttggc gcaatcgatg acgatccgca ggccggccaa agagagctga
      481 cgcgggaagg tcgccttgac gatctcgaca tnnnnnnnnn ngggcctggg cgtcgtcgat
      541 gcgcttgacg cggcccaggc cgcgcggcgg cgccaggcct tcctgcaggc cctcgtccat
      601 cagggcctcg atcttcagtt cctgctcgtc cgacagcttg tagccgtcgg ggccgaacag
      661 cttgatgccg ttgtcagcaa aattgttgtg gctggcgctg atcatcacgc ccagatcggc
      721 gcgcatcgag cgggtcatca tggctacagc cggggtgggc agcgggccaa acaggcgcac
      781 gtccatgccg acgctggcga agccggccac cagggccggc tcgatcatgt agcccgagag
      841 ccgggtgtcc ttgccgatca ccacccggtg gcggcggtca tcctgcgaac gaaacagttt
      901 tccggccgcc aggccgacgc gtagggcgac ctcggcggtc atcggatgct ggttggcctg
      961 gccgcggatg ccgtcggtgc cgaaataggc gcgcttgctc atggtggttc tggtctcggt
     1021 taggctcgat agggatcgcg aaacgatccg aaatgcagat ttatgtgggc caaccctgca
     1081 cgaatgctaa agcccggccg acgactatac ccgcgccggt agccataatc cccgctggcg
     1141 cgatttacaa aggaccatgg catgtgcggc atcattggca tcgtcggaaa ccagcccgtg
     1201 gccgaccgtc tggtcgaaag cctcaagcgc ctggaatatc gcggctatga ctctgcgggc
     1261 atcgccgggc aggtggatgg cgtgctgcag cgcccccggg aagctccggg aactagaaaa
     1321 tgtgctggct aacgagccgc tgatcgccac caccggcatc ggccataccc gctgggccac
     1381 ccacggcgcg ccgaccctgc gcaacgcgca cccgcacatc gtcggcaagg tggccgtggt
     1441 ccataacggc atcatcgaga actttgccga gatgaaggcc gagatgatcg ccgccggccg
     1501 cgagttccag agcgacaccg acaccgaggt cgtcgcccac cttctggacg ccgccctggc
     1561 caccggcctg tcgccgctgg acgccttcaa ggccaccctc gaccgcctgc gcggcgcctt
     1621 cgccctgtgc gtgctgatcg gcggcgagga tgaggtgatc ctggcggccc gcaacggtcc
     1681 gcccctggcc gtgggctatg gcgacggcga gatgtttgtg ggctccgatg gcctggcgct
     1741 cggccccttc accaatcgcg tcgcctatct gcaggatggc gactacgtca tcctcgacca
     1801 ccaggccgcc aagatctttg accagagcgg caagccggct gaccggccga tcaagatcgt
     1861 cccggcctcg gccgtgctga tggagaaggg caactatcgg cacttcatgg agaaggaaat
     1921 ccatgaccag cccgagggct gccagcgcac catcgccgcc tatgtcgacc ccatcgcggc
     1981 ccgcgccgcc gtgcccggcg agatcgactg ggccagtctg gagcgtatcc agatcgtggc
     2041 ctgcggcacc tcctatatcg ccggcatgat cggtcgctat ctgatcgagc agctggccga
     2101 cctgccggtg gatgtcgaga tcgcctccga attccgctac cgccagccgg ccatccgccc
     2161 cggcgctctg gccatcgcca tgtcgcagtc gggcgagacc gcagataccc tcgcggccct
     2221 gcgctattgc caggcggccg gcatgaagag cgccgtggtc gtcaacgcca ccgaatccac
     2281 catggcccgc gaggtcgacg tggtctggcc gatccattgt gggcccgaga tcggcgtggc
     2341 ctcgaccaag gccttcaccg cccaggtcag cgtgatgatc gccctggcca ttgcggccgc
     2401 caaggcgcgc ggccgcatcg atgacgccga ggagcagcgg ctggtcaagg tgctgctgga
     2461 agccccgcgc ctgatcgccg aggccatcgg cctggaagac gccatcaagg acatcgccgc
     2521 cgaggtcgcc aaggcccgcg acgtgctcta tctcggccgc ggcccgatgt cggccctggc
     2581 actggaaggc gcactgaagc tgaaggaaat cagctatatc cacgccgagg gctatgccgc
     2641 cggcgagctg aagcatggcc ccatcgccct ggtcgatgac cagacgccga tcgtgatcct
     2701 tgcccccttc gacagctggt tcgagaaatc ggcctcgaac atgagcgagg tcatggcgcg
     2761 cggcggccag gtgatcttca tcaccgacac agaaggcgca aagcacgccc cggccggcgc
     2821 caaggtggtg atcaccgctc cggcctgcga cccgctgatc tcggccctgg tgatgtcggc
     2881 cccgatccag ctgctggcct atcacgtggc cgtg
//
LOCUS       DAOMEO010000318         2913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3748_length_2913_cov_2.985654, whole genome shotgun sequence.
ACCESSION   DAOMEO010000318 DAOMEO010000000
VERSION     DAOMEO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_2913_cov_2.985654"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH31_08165"
     CDS             complement(<1..209)
                     /locus_tag="PLH31_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HQR89223.1"
                     /translation="MSGDSSGVVNRFLEVFSRYIDSGFGLVGGEVAFLATTLVAIDIT
                     LAALFWAWSPGEEILARLVKKTLFVG"
     gene            complement(206..460)
                     /gene="trbK-alt"
                     /locus_tag="PLH31_08170"
     CDS             complement(206..460)
                     /gene="trbK-alt"
                     /locus_tag="PLH31_08170"
                     /inference="COORDINATES: protein motif:HMM:NF039468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative entry exclusion protein TrbK-alt"
                     /protein_id="HQR89224.1"
                     /translation="MTGRPDPCRLAIAIGLIVALVAVMAAMALVHGRKASAPKADRSA
                     SPLSGELRRCGDLGEKARGDARCRAAWDESRARFFGERGS"
     gene            complement(457..1179)
                     /gene="trbJ"
                     /locus_tag="PLH31_08175"
     CDS             complement(457..1179)
                     /gene="trbJ"
                     /locus_tag="PLH31_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HQR89225.1"
                     /translation="MKSVISRRNLMLGLVSLGAVAAPAAAQLTVNDPMNYAQNVVQAA
                     RSLEQINHQIASLQNEATMLVNQARNLESLPVSSLTRLQAQVSRTQALLGQAQRLAYD
                     VDAVETAFKGRYGAARLGATEQDLVARAQERWATSVGAFEDALKVQAGVVGGLETSRA
                     EMQTLVTASQNATGALQAAQAGNQLLSLQSQQLAQITALLAAQGRAQALEASDRAAAQ
                     QDARARFQAFMGKSGATQAPKP"
     gene            complement(1176..>2913)
                     /locus_tag="PLH31_08180"
     CDS             complement(1176..>2913)
                     /locus_tag="PLH31_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022584.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR89226.1"
                     /translation="TATWPGLLDELNRLAFAYRWSTRAVCLDKTDALAVLGKIRRQWF
                     AKRKSVAALIKEVMTNEPANLLDGDAANKAIEADAALQDLGGDGVAFAYVTATVVVWD
                     EDPALAEEKLRLVEKVVQGRDFTCIRETLNAVEAWLGSVPGNAYANVRQPPISTLNLA
                     HMIPLSAIWAGPARNDHLGGAPLLLARTEGSTPFRLCLHVGDVGHTLVVGPTGAGKSV
                     LLALMALQFRRYDRAQIFAFDFGGSIRAATLAMGGDHHDLGAALAPDEDGAVRLQPLE
                     RVDDPAERAWASAWLEAIFAREGVALDPAAKALVWSALGSLASSPAAERTLTGLVALL
                     QSASLKQALRPYCLGGPFGRLLDGEFEHLGVADVQAFETEGLLGSTAAPAVLAYLFHR
                     IEGRLDGRSSLIIIDEGWLALDDPAFGAQLREWLKTLRKKNASVVFATQSLSDIEASP
                     LAAAIIESCPTRILLPNPRALEPQIAAIYRRFGLNDRQIEILSRATPKRDYYAQTRAG
                     NRLFELGLGPIGLAVCAASSKDDQAAITEVLTTSGPADFAAAWLRRRGLDWASILLSS
                     HAVASLQSEKQS"
BASE COUNT          504 a          993 c          971 g          445 t
ORIGIN      
        1 cccacgaaca gtgtcttctt cacgagcctg gccaggatct cctcacccgg cgaccaggcc
       61 caaaacaaag ccgccaaggt gatgtcgatc gcgaccaggg tcgtggccag gaacgccacc
      121 tcgccgccca ccagtccaaa cccactgtct atgtagcggc tgaagacctc caggaagcga
      181 ttgacgacgc cgctgctgtc gccgctcacg agcccctctc cccgaagaag cgcgcccggc
      241 tctcatccca tgccgcccgg cagcgcgcgt cgccgcgggc cttctcccca aggtcgccgc
      301 agcgcctaag ctcgcccgac aaaggactgg ccgaacgatc ggccttcggc gctgaggcct
      361 ttcgcccatg gaccagggcc atcgcggcca tgaccgcgac cagcgccacg atcagtccaa
      421 tggcgatggc cagacgacaa ggatcaggtc gcccggtcat ggcttgggcg cctgcgttgc
      481 accgctctta cccatgaagg cctggaagcg cgccctggcg tcctgttgcg cggcggcgcg
      541 atcggacgct tccagcgcct gggcgcgtcc ttgagccgcg agcagcgcgg tgatctgcgc
      601 cagttgctgc gattgcaggg acagcagttg attgccggcc tgggcggcct gcagcgcgcc
      661 tgtggcgttc tggctggccg taaccagggt ctgcatctcg gcccggctgg tctccagccc
      721 accgaccacg ccggcctgga ccttcagggc gtcttcaaaa gcgccgaccg aggtcgccca
      781 gcgctcttgc gcgcgcgcca ctagatcctg ctcggtggcg ccgaggcgcg cagcgccata
      841 ccgacccttg aaggcggttt cgacagcgtc gacgtcatag gccagcctct gggcttggcc
      901 gagcaaggcc tgggtgcgcg acacctgcgc ctgaagccgg gtcagggatg agaccggcag
      961 gctttcgaga ttgcgggcct gattgaccag catggtcgct tcgttttgca acgaggcgat
     1021 ctggtgattg atctgctcca ggctacgcgc cgcctgaacc acgttctgcg cgtagttcat
     1081 cgggtcgttg acggtgagct gggccgccgc gggcgcggct acggctccaa gggacaccaa
     1141 gcccaacatg agatttcgcc gtgagatcac tgatttcatg actgcttctc cgattgcaat
     1201 gaggcgacgg cgtgggaact cagaagtatg gacgcccagt cgagcccgcg acgccgcagc
     1261 caggcggcgg cgaagtcggc cggcccggac gtcgtcaaaa cctcggtgat ggcggcttgg
     1321 tcgtccttgg aggatgcggc gcaaaccgcc agaccgatcg gacccagccc cagttcgaac
     1381 aatcggttcc cggcccgggt ctgggcgtag tagtcgcgct tgggcgtggc ccggctgagg
     1441 atttcgattt gccgatcgtt gagcccgaag cgccgataga tcgcggcgat ctgcggctcc
     1501 agggcgcgcg gattggggag cagaatacgg gtcgggcagc tctcgatgat cgccgccgcc
     1561 agggggctcg cctcgatgtc ggacagcgac tgggtcgcga aaaccaccga cgcgttcttc
     1621 ttgcgcagcg tcttgagcca ctcgcgaagc tgcgcgccga aggccgggtc atcgagagcc
     1681 agccagccct cgtcgatgat gatcaggctg gaccgaccat ccaagcgccc ctcgatgcga
     1741 tgaaagagat aggccaggac ggccggcgcg gcggtcgaac ccagcagccc ctcggtctcg
     1801 aacgcctgga catccgcgac gcccagatgc tcgaactcgc catccagaag ccggccgaac
     1861 ggtccgccca ggcaataggg ccgcaaggcc tgtttgagcg acgccgattg cagcagggcg
     1921 accaagccgg tcagggtccg ttcggcagcc ggcgaggagg ccaggctgcc caaggcgctc
     1981 cagaccaggg ccttggcggc cggatcgagc gccacgcctt cgcgggcgaa aatcgcctcg
     2041 agccaggccg acgcccaggc ccgttcggcg gggtcatcga cacgctctag aggctgcagc
     2101 cggacagccc cgtcctcgtc aggcgccaag gctgcgccaa gatcgtgatg atcgccgccc
     2161 atggccaggg tggcggcgcg gatcgaaccg ccaaagtcga aggcgaagat ctgggcgcgg
     2221 tcatagcgcc gaaactgcaa ggccatcagg gccaggagca cgctcttgcc cgcgccggtt
     2281 ggcccgacga caagcgtatg gccaacatcg ccgacgtgaa ggcaaagccg aaacggcgtc
     2341 gagccttcgg tgcgggccag cagcagcggc gccccgccca aatgatcgtt gcgtgccggc
     2401 ccagcccaga tcgccgacag cgggatcatg tgggccaggt tcagagtcga gatcggcggc
     2461 tggcggacat tggcgtaggc gttgcccggg accgaaccga gccaggcctc gaccgcgttc
     2521 agggtctcgc gaatgcaggt gaagtcgcgc ccctggacga ccttctccac caaccgcagc
     2581 ttctcctcgg ccagggcggg atcctcgtcc cagaccacga ccgtggcggt gacataggcg
     2641 aaggcgacgc catcgccgcc caggtcctgc agcgccgcgt cggcctcgat cgccttgttg
     2701 gcggcgtcgc cgtcgagaag gttggccggc tcgttggtca tcacctcctt gatcagggcg
     2761 gcgaccgact tgcgcttggc gaaccactgg cgtcggatct tgcccagcac ggccaaggcg
     2821 tcggtcttgt ccaggcagac cgcccgggtc gaccaacgat aggcgaaggc caggcgattg
     2881 agctcatcga gcaggcccgg ccaggtcgcc gtc
//
LOCUS       DAOMEO010000319         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3758_length_2909_cov_2.792221, whole genome shotgun sequence.
ACCESSION   DAOMEO010000319 DAOMEO010000000
VERSION     DAOMEO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3758_length_2909_cov_2.792221"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLH31_08185"
     CDS             complement(<1..424)
                     /locus_tag="PLH31_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89227.1"
                     /translation="MKPAKAEPVAHETELLRLTLTSDAERRLGLQTVAIGSGAARRVR
                     AAHGEIVAAPYAGGLPTASGTDLGTLAANQTRTDGDVARARAEAAAVLGVARASLATA
                     QAQRGHAVEGLLIRPPSLLLIVHRFISSTLPAKNCEAWL"
     gene            complement(746..>2909)
                     /locus_tag="PLH31_08190"
     CDS             complement(746..>2909)
                     /locus_tag="PLH31_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HQR89228.1"
                     /translation="RTCLGSQWAQVGDALAALPSAALPAGEAVVGLTLHPSYLAKSYY
                     PAHLLKDLQLRHMGSRAVHLRPEKITSKRANDDPRPLLAPLIYVAGAAERIEQFSAGL
                     DQWTPLSEYVRDDFRKIEEVALPKADRFKPLAPALAARKDPIPLEIVLHDDDEEDGII
                     LPAFTAFCQSLGVKLILERRRRTGGLVFLPARALVDDIPGLLDFTFLRAVRGMPVLKP
                     LDPILRSQIGGFKVALPQEDAIVPDLAVAIFDGGLPEKHGLERWVTAHDAPGVGAPID
                     GGPRHGIGVTSAFLFGPLAEGETPPRPFANVEHWRVIGDDTKADDFALLAVLERIENV
                     ISSQRYDFINISLGPDSAIEDDDVNSWTSTIDALLADGETVATVACGNNGEADTLTGL
                     HRIQPPSDGVNIISVGAANKPFGAWTRAPYSACGPGRSPGFVKPDLIAFGGSAGAPFL
                     MLDGSSPGGASGNCGTSFASPLVMRCAAGVRAQFAQHLWAPTVKALMIHQADPAQHAR
                     PEVGWGRLSHGLGDLVLCEDGEAHIVYQRLMPTSGSVRMELPVPPELTGMFEFKATFC
                     IYAEIDPEDSLNYTRAGLDIQFRPDSVTVPPPYRKDGKLITPTVPSSTGFFSSADLYA
                     TEFMRREDAHKWETTFTKTKKMRSTSLNRPVFDVGYAPRQHGQGATRSANLKLALVLT
                     IRQAKTKNLYDRIVATAAGRLQPLRARAGLQVPARLRR"
BASE COUNT          551 a          875 c          958 g          525 t
ORIGIN      
        1 tgagccaagc ctcgcaattt ttagcgggca gcgtcgagga tatgaaccga tggacaatca
       61 gcagtaaact aggtggccgt atcaacaggc cttcaacggc gtgtccgcgc tgcgcctggg
      121 ccgtagccag actggcgcgg gcgacgccca agacggcagc ggcctcggcg cgggcgcgag
      181 cgacgtctcc gtcggtgcgc gtctggttcg cagccagggt tccaagatcg gttcctgacg
      241 cggtcggcag cccgccggca tagggcgcgg cgacgatttc accgtgggct gcccgcactc
      301 tgcgggcggc gcccgacccg attgcaaccg tctgaaggcc cagccgccgt tcggcgtccg
      361 aggtcagggt cagcctcaag agttcagtct catgggcgac cggctcggcc ttggccggct
      421 tcacggccgc agacttatcg tcagagccgc acgcagcgag ggcagcgcag acgccgacgg
      481 cggcccaacg tgcgcgaggc aggcggttca cgtccggcgt caagaggggc ctcctgcaca
      541 gcttggcagg tctccttcat gctcggtttg gtttcgcgga cactctccat ttctatacaa
      601 tctgcgaagg tagtggccgt cgatcgggga ccgcgccaaa gccgcccagt tatggccgct
      661 tatattgctg agccgcccca gaaaaatagt ctgacaacta cggttgtcag acgagccatc
      721 tcgctcttgc gggtggggtg ttcggttatc tccgcagtcg agcaggaacc tgcagtccgg
      781 ctctggcgcg aagcggctgc agccgcccag cagccgtggc aacgatccga tcatagagat
      841 tcttggtctt ggcctggcgg atggtcaaaa caagcgcgag cttgaggttt gctgaccgtg
      901 tggcgccttg gccatgctgt cgaggagcgt atccgacgtc aaagacaggc cggttcaacg
      961 aggtcgacct cattttcttg gtcttggtga aggtcgtttc ccacttgtga gcatcttcgc
     1021 gccgcatgaa ctctgtggcg tacaaatccg ccgaggagaa gaagccggtg ctggagggaa
     1081 cggtgggcgt gatcagttta ccgtccttcc tgtagggcgg cggcacggtg acgctgtcgg
     1141 gacgaaactg aatatcgagg cccgcgcgcg tgtagttgag agagtcctca gggtcgatct
     1201 cggcatagat gcagaacgtg gccttgaact cgaacatgcc ggtcaattca ggcggcacag
     1261 gcaattccat ccgcactgaa ccgctcgtgg gcatgaggcg ctgatagaca atgtgcgcct
     1321 cgccgtcctc gcaaagcacc aaatcgccca ggccatgcga caggcgcccc caaccgacct
     1381 cgggtctggc gtgttgggcc ggatcagctt gatggatcat caacgccttt accgtcggag
     1441 cccaaagatg ctgggcaaac tgcgcgcgga caccggcggc gcatcgcatc accagcggcg
     1501 aagcaaagct cgtgccgcag ttgccgctgg cgccgcctgg gcttgagccg tcgagcatta
     1561 gaaaaggtgc gccagccgag ccgccgaacg cgatgaggtc tggtttgaca aagcccgggc
     1621 tgcgaccggg cccgcatgcg ctgtacggcg cccgtgtcca ggcgccgaaa ggcttgttcg
     1681 cggcgccgac cgagatgatg ttgaccccgt ctgacggggg ctgaattcga tgcaggccgg
     1741 taagggtgtc agcctcgcca ttgttgccac aagccaccgt ggcgacggtt tcgccgtcgg
     1801 ccagaagcgc gtcgatggtc gacgtccagg agttgacgtc gtcatcctcg atggcgctgt
     1861 cgggtcccaa gctgatgttg atgaagtcgt agcgctggga cgagatcaca ttctcaatcc
     1921 gctcgagcac cgccagcagg gcaaaatcgt cagctttggt gtcgtcgccg atgacccgcc
     1981 agtgttcgac gttggcgaag ggacggggtg gcgtttcgcc ttcggcgagc ggcccgaaca
     2041 ggaaagccga ggtcaccccg atgccgtggc gcggtccacc atcaattggc gcgcctacac
     2101 caggagcgtc gtgagctgtt acccagcgtt cgagaccatg cttttcggga aggccgccat
     2161 cgaagatggc tacggccagg tcgggtacga tcgcatcctc ctgcggcagg gcgaccttaa
     2221 aaccgcctat ctgcgaacga aggattggat cgagcggctt gagaacaggc atcccgcgaa
     2281 cagctcgcaa gaacgtaaaa tcgagcaagc cgggtatgtc gtcgaccagg gctcgagcgg
     2341 gcaagaagac caggccgcct gtcctgcggc gccgctcgag tataagtttc acgcccaggg
     2401 attggcagaa agccgtgaag gccggcagaa taatcccgtc ttcctcgtcg tcgtcgtgga
     2461 gcacgatttc caaggggatc ggatctttgc gcgccgccaa ggcgggtgcg aggggcttaa
     2521 agcgatcagc tttggggagc gcgacctctt caatcttgcg aaaatcgtcg cgaacatact
     2581 ccgacagcgg ggtccattgg tccaggccag cggaaaactg ctcgatacgt tcggcggctc
     2641 ctgcgacata gatcaacggt gccagcagcg gtcgcggatc gtcattggcc ctcttggagg
     2701 taatcttctc cggccgaaga tgcacggccc ggctacccat gtggcgaagc tgcaggtcct
     2761 tgagcaggtg ggccgggtag tagctcttgg ccagatagga ggggtgaagc gtcaagccca
     2821 cgaccgcttc gccggcggga agcgccgccg aaggtagggc tgcgagcgca tcacccacct
     2881 gtgcccactg cgatccgagg cacgtgcgc
//
LOCUS       DAOMEO010000320         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3787_length_2885_cov_2.634276, whole genome shotgun sequence.
ACCESSION   DAOMEO010000320 DAOMEO010000000
VERSION     DAOMEO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3787_length_2885_cov_2.634276"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..757
                     /gene="gspN"
                     /locus_tag="PLH31_08195"
     CDS             53..757
                     /gene="gspN"
                     /locus_tag="PLH31_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HQR89229.1"
                     /translation="MSNRAFVAILIAALVLMTVILAPVGLLTGAMAGSSRVGMTSASG
                     TVWAGRLNDVAIAGNPVGDLKVGLSPFSLATGQMRLSLRRTPLADARVVLLKGGRATG
                     VEGLNLRTPIDLTAAGLPLSGVVGFTDVAGVFREERCLRAKGRVQLILAGDSPLRGAV
                     LSGEPVCRSGVWSATLVGPAADGKATLAIRIDGKGRYQLEIMVATVNEVMIQGLLGSG
                     FLRDAVGARRTVEGRL"
     gene            complement(764..1537)
                     /locus_tag="PLH31_08200"
     CDS             complement(764..1537)
                     /locus_tag="PLH31_08200"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HQR89230.1"
                     /translation="MTIDHLWAVSTLIFAPFIGSFIGLLTLRLPAGRPWALSRSACDA
                     CGRTLGVVDLIPLLSFLVLRGRCRTCHAAIPPRYLLLEGGCLLIALWSALTQTGVMVP
                     VTAILGWGLLLIAIIDAEHLWLPDRLTLPLGLVGALLTVGFANASPWTPLLGAAAGFC
                     GLAAIAWLYKRVRGFDGMGGGDPRLLGAIGAWVGWQGLPSVLIWACVAGLSVAASKAI
                     VRRRLAPGQELPFGAFLAIGAWLTWLWGPLHVLIRDGLA"
     gene            1717..2817
                     /locus_tag="PLH31_08205"
     CDS             1717..2817
                     /locus_tag="PLH31_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C1-like protein"
                     /protein_id="HQR89231.1"
                     /translation="MLSFVAALLMAASPAADPRMNDLTAVGTHNSYKLGVPPEEMAIM
                     VAARGQPVLGLDYAHRPLAEQLDAGARQLELDVVADPLGGRYAKPLTALGAGAVLAPD
                     QTAALSRPGFKTLHVPDVDFRTSCLTFITCLTQVKAWSKAHPEHVPILILINAKEGPA
                     SLPGGVTPLAFDEALFDALDAEIRTVFVDSELVTPDQVRGKRATLREAVLADGWPRLS
                     SSRGKVFFALDESPEKVALYRGARRSLEGRAMFVNTDEASPDAAYLTLNDPVAQKDRI
                     AAAVKAGFMVRTRADADTWAARANDVRQRNAALASGAQYVSTDYMWPDARFEGGYTVR
                     LPGSDAAICNPVRAAELCNGAALERVAGPTPD"
BASE COUNT          479 a          972 c         1009 g          425 t
ORIGIN      
        1 ggctggacgg cggcgtcatt gaggccgaaa tcacctttgc cggggccgcg cgatgagcaa
       61 ccgagctttt gtcgcgatcc tgatcgcggc cctggtgctg atgaccgtga tcctggcccc
      121 ggttgggctg ttgacgggcg ccatggcggg ctcaagccgt gttggcatga ccagcgccag
      181 cggcacggtg tgggctgggc gcctgaatga cgtggccatc gcgggcaatc cggtcggaga
      241 cctgaaggtg ggcctctcgc ccttttctct tgcaacgggc cagatgcggc tgagcctgcg
      301 gcggacgcca ctggctgacg cgcgggtggt gctgctgaag ggcgggcggg ccacgggggt
      361 tgaggggctg aacctgcgga cgccgatcga cctgacggcg gcgggactgc ccctctcggg
      421 cgtggtgggt ttcacggacg tcgctggcgt gttccgcgag gagcgatgcc tccgggccaa
      481 agggcgcgtt cagctcattc tggcggggga tagccccctc cgcggcgccg tgctcagcgg
      541 cgagccagtc tgtcgcagcg gcgtctggag cgcgaccctg gtcgggcccg ccgcagacgg
      601 caaggcgacg ctggcgatcc ggattgacgg caagggccgc taccagttgg aaatcatggt
      661 cgcgacggtc aatgaagtca tgatccaggg tctgctgggt tcgggattcc tgcgggatgc
      721 ggtcggcgcg cgacgcactg tggaagggcg cctgtagggc gcgtcaggcc aggccgtcac
      781 ggatcagcac atgcagcgga ccccatagcc aggtcagcca ggcgccgatg gccagaaacg
      841 cgccgaacgg caattcctga cctggcgcca ggcgacggcg cacgatcgcc tttgaggcgg
      901 cgacgctgag gccggcgacg cacgcccaga tcagcaccga gggcaggccc tgccatccca
      961 cccaggcccc gatggccccc agaagccggg gatcgccgcc gcccatgccg tcaaaccccc
     1021 ggactctctt gtagagccag gcgatcgccg ccagcccgca aaagcctgcc gccgcgccca
     1081 gcagcggtgt ccagggggag gcattggcga acccgaccgt caggagggcc cccaccaggc
     1141 ccagcggcag ggttagcctg tcgggcagcc acaggtgctc ggcgtcaatg atcgcgatca
     1201 gcagcaggcc ccaccccagg atcgccgtca ccggaaccat cacgccggtc tgggtgagcg
     1261 cgctccagag ggctatgaga aggcagccgc cctccagcag gagatagcgg ggcgggatcg
     1321 cggcatggca ggttctgcag cgtcccctca gaaccagaaa gctgagcagg gggatcaggt
     1381 cgacaacgcc gagggtgcga ccgcaagcgt cgcacgccga acgcgacaac gcccagggac
     1441 gacccgccgg caggcgcagc gtcagcaggc cgatgaagct gccgatgaaa ggcgcgaaaa
     1501 tcagggtcga aacggcccag aggtggtcga tggtcaagcc tgtagtccca aatccgccgg
     1561 caagccttgg ccgaaagccg cgtggaccgc aatcactcag gcggctctgc ggccattttc
     1621 gtggcagcat cgacggcgcg ccccgccgcg ctccggacga cgacgtcaaa ccgtcatcgg
     1681 gtcgggctag tcggaccgtg acccttggag gatcccatgc tgagtttcgt cgccgcgctc
     1741 ctgatggccg ccagtcccgc cgccgacccg cgcatgaacg atctgacggc ggtgggcacg
     1801 cacaacagct acaagctggg tgttccgccc gaagagatgg cgatcatggt cgccgcacgc
     1861 ggccagccgg ttctgggact ggactatgct caccgtcctc tggccgagca gttggacgcc
     1921 ggcgcccggc aactggagct ggacgtcgtc gccgacccac tgggcggtcg ctacgccaag
     1981 ccgctgaccg ccctcggtgc gggtgcggtc ctggctccgg accagaccgc agccctctcc
     2041 aggcctggct tcaagaccct gcacgtgccc gacgtcgatt tccgcacctc gtgcctgacc
     2101 ttcatcacct gcctgaccca ggtgaaggcc tggtcaaaag cccatcccga acatgtgccg
     2161 atcctgatcc tgatcaatgc caaggagggg ccggccagcc tgccgggcgg cgtcacgccc
     2221 ctggcctttg acgaggcgct cttcgacgcc ctggacgccg agatccggac ggtgttcgta
     2281 gacagcgaac tggtcacccc tgaccaggtg cgcggcaagc gcgcgaccct gcgcgaggcg
     2341 gtgctggccg acggctggcc caggctatcg tcgtcgcgcg gcaaggtgtt tttcgccctt
     2401 gatgaaagtc ccgagaaggt cgccctctat cgcggcgcgc ggcgctcgct ggaggggcgg
     2461 gcgatgttcg tcaataccga cgaggcctca ccggatgcgg cctatctgac cctcaacgat
     2521 ccggtcgcgc agaaggaccg tatcgccgcc gccgtgaagg ccggtttcat ggtgcgcacc
     2581 cgggcggacg ccgacacctg ggcggcgcgg gccaatgacg tccgccagcg gaacgccgca
     2641 ctggcgagcg gcgcgcagta tgtctcgacc gactacatgt ggccggatgc gcggtttgag
     2701 ggcggctata cggtgcggct acccggcagc gacgccgcga tctgcaaccc cgtccgcgcc
     2761 gccgagctgt gcaacggggc ggcgctcgag cgtgtggcag ggccgacgcc cgactgacct
     2821 tcccgtggca gaagcttcga gcattccgtc ggctcgcaca taagagtggt ctcgcctata
     2881 taggc
//
LOCUS       DAOMEO010000321         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3795_length_2878_cov_3.244775, whole genome shotgun sequence.
ACCESSION   DAOMEO010000321 DAOMEO010000000
VERSION     DAOMEO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_2878_cov_3.244775"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLH31_08210"
     CDS             <1..370
                     /locus_tag="PLH31_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR89232.1"
                     /translation="FGAWLRTIAINKARDRGRRMMVRRAIFGSKGLDDTEALETADPE
                     PGAEHAVLEDEQARRLDRAVSRLPAALKEALILTAFEGFSQQQAAQSLGVSVKTIETR
                     VYRARKLLIDQLDPDLRPGA"
     gene            complement(378..848)
                     /locus_tag="PLH31_08215"
     CDS             complement(378..848)
                     /locus_tag="PLH31_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQR89233.1"
                     /translation="MDHKDPAMEGKHYGKLALMIGLSFISMYILMYAMVDKLSSIHPS
                     LNQAYMAGLMTAPMVVLELLLMSMMYARRRLNAVILVVSVVAGLACFIAIRQQFLIGD
                     REFLRSMIPHHSGAILMCGKAPIRDPELRKLCVAIIQGQQAEIDQMNAMLKASR"
     gene            1085..1441
                     /locus_tag="PLH31_08220"
     CDS             1085..1441
                     /locus_tag="PLH31_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HQR89234.1"
                     /translation="MTLIRNITFAPFRFGRAAQSAMLAAGVGLLLATPAWAAGGGTAM
                     PWEGPLTTVMQSLSGPVAKAIGIIAIVLTGLGFAFAEGGSAMRKGIGIVFGLAIAFTA
                     TTFVSTFFNLTAGAAF"
     gene            1443..1709
                     /locus_tag="PLH31_08225"
     CDS             1443..1709
                     /locus_tag="PLH31_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HQR89235.1"
                     /translation="MAQSEVEGFEIAFHSSLSEPVTIAGVPRMIAVLNGTLTAVLALG
                     LQVPLIGLPVGLAIHVACYWLNKRDPYFFDALGRHIRQRPYLDA"
     gene            1810..>2878
                     /locus_tag="PLH31_08230"
     CDS             1810..>2878
                     /locus_tag="PLH31_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275049.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR89236.1"
                     /translation="MLYLREYRPKADRLFDHLPWVALIGPGLILNKDGSFQKTLAFRG
                     PDLASSTDAGLVATRAQLNNALRRLGSRWCLHIEALRAASQDYPASQFPDLVSDLVDE
                     ERREAFEGHERHFESRYFLTFTYLPPEEAVSTAESLLLENAPSGRGAAGMYRAALSDF
                     LSTVRQIADILTAIMPEVHELSDDETLTYLHGCISTKRHYVKAPETPAYLDAFLTDDD
                     FQGGLLPRLGGSYVRTISVRAYPATSSPGLLDRLNELGISYRWVCRFLPLDKEDARKA
                     VTTVRKRWFAKRKGVMALLKEAITREPSVLEDPDATAKSMDADAALAILGGDYASIGY
                     FTPTVTLMDTDPDRLADRVREV"
BASE COUNT          485 a         1000 c          881 g          512 t
ORIGIN      
        1 gttcggggcc tggctgcgaa ccatcgccat caacaaggcg cgcgatcgcg gacgccggat
       61 gatggtgcgg cgcgccatct tcggttcaaa aggcctggat gataccgagg ccttggaaac
      121 ggccgaccct gaacctgggg ccgagcacgc ggtgttggaa gacgaacagg cgcgacgcct
      181 cgaccgcgcc gtcagccgcc tgccggcggc gctgaaggag gccttgatcc tgacggcctt
      241 tgaggggttc agccaacagc aagcggccca gagcctcggc gtctccgtca agacgatcga
      301 gacgcgcgtc taccgcgccc gcaagttgct gatcgaccag ctagacccgg acctgcgccc
      361 gggcgcgtga atcggcctca gcgtgacgcc ttcagcatgg cgttcatttg atcgatttcc
      421 gcctgctgac cctgaatgat cgcgacgcac agctttctca attcggggtc gcggatcggg
      481 gccttgccac acattaggat cgcccccgag tggtgcggga tcatcgagcg cagaaactcc
      541 cggtcgccga tcaggaattg ctgacgaatg gcgatgaagc aggccagtcc ggcgaccacg
      601 ctgaccacta gaatcacggc gttcaagcga cgccgagcgt acatcatgct catcagcagg
      661 agttcgagca ccaccatcgg cgcggtcatc aacccggcca tgtaagcctg gttgaggctg
      721 gggtggatgc tcgaaagctt gtccaccatc gcgtacatca ggatgtacat cgagatgaag
      781 gacagcccga tcatcagggc gagcttgccg tagtgtttcc cctccatcgc cggatccttg
      841 tggtccatgg acagcactcc tcttcggcag gggatagtcc ccctagggat acgcgcgttg
      901 cggagcccac ccctctgcct gcgccattca ggttcgattc gtgccgttgc tcggacgaaa
      961 gtcgtcaaaa ggtatcgtga cgcacctttt ttatataaaa agcacttgac ccgagcgcgt
     1021 gagggtctca atcgtgggat tagcccggca ttcgcgggcg ttcggcatca ggaagccccc
     1081 cacgatgact ctgatccgaa atatcacctt cgcgccgttt cgcttcgggc gcgccgccca
     1141 atccgcgatg ctcgccgccg gcgtcggcct gcttctggcc actccagctt gggctgcggg
     1201 cggcggcacc gccatgccat gggagggtcc gctcaccacg gtgatgcagt cgctctccgg
     1261 tcccgtcgca aaggcgattg gcatcatcgc catcgtcttg accgggctgg gcttcgcctt
     1321 cgccgaaggt ggctcagcca tgcgaaaggg catcggcatc gtcttcgggc tggccatcgc
     1381 cttcaccgcc acgaccttcg tcagcacctt cttcaacctc accgccgggg cggcgttctg
     1441 aggtggcgca gtccgaagtc gagggcttcg agatcgcctt ccacagctcc ctttcggagc
     1501 cggtgacgat cgcgggcgtg ccgcgaatga tcgccgtgct gaacggcact ctgacggcgg
     1561 tcctggcgct gggcctccag gttcccctga tcggcttgcc cgtcggcctg gccattcacg
     1621 tcgcctgcta ctggctcaac aagcgcgacc cctacttctt cgacgccctc gggcggcaca
     1681 tccgccaacg gccctacctg gacgcctgac cacgggcgcg cccgtcgccg gcgcgcgact
     1741 gaattctacc ccgcgcccat ctgggcgact tcaacgcccg atccggcccc caaccgcaga
     1801 ggacagccca tgctctacct gcgcgaatac cgtcccaagg ctgatcgtct gttcgatcat
     1861 ttgccctggg tggcgctgat cggtccgggc ctgatcctca acaaggatgg cagctttcag
     1921 aagaccctgg cctttcgggg tcccgatctg gccagttcga cggacgccgg cctggtcgcc
     1981 acccgcgccc agctgaacaa cgcccttcgc cgcctgggtt cgcgctggtg cctgcacatc
     2041 gaggcgctac gggcggcgtc gcaggactac ccggccagcc agtttcctga tctcgtctcc
     2101 gacctggtcg acgaggagcg ccgcgaggcg ttcgagggtc acgagcggca ctttgagagc
     2161 cgctacttcc tcaccttcac ctacctcccg cccgaggagg ccgtcagcac ggccgagagc
     2221 ctgctgctgg agaacgcccc ttcagggcgc ggcgcggccg gcatgtaccg cgcggccctg
     2281 agcgatttcc tcagcacggt gcgccagatc gccgacatcc tgaccgcgat catgccggag
     2341 gttcacgaac tctccgacga tgagacactc acctatctgc atggctgcat ctccaccaag
     2401 cgccactacg tcaaggcgcc ggagaccccg gcctatctcg acgccttcct gaccgacgac
     2461 gatttccagg gcggcctcct gccgcgcctg ggcggcagct atgtgcggac catctcggtg
     2521 cgcgcctatc cggcgacctc ttcgccgggc ctgctggacc gcctgaacga gctggggatc
     2581 agctaccgct gggtgtgccg cttcctgccc ctcgataagg aggacgcccg caaggcggtg
     2641 accaccgtcc gcaaacgctg gttcgccaag cgcaagggcg tcatggcgct actgaaggag
     2701 gccataaccc gggagccctc ggtcctggag gatcccgacg ccacggccaa gtcgatggac
     2761 gccgatgcgg cgctggccat cctcggcggc gactacgcct cgatcggcta cttcacgccg
     2821 acggtgacct tgatggacac cgatcccgac cggctggccg accgggtccg cgaggtcg
//
LOCUS       DAOMEO010000322         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3799_length_2874_cov_2.700603, whole genome shotgun sequence.
ACCESSION   DAOMEO010000322 DAOMEO010000000
VERSION     DAOMEO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_2874_cov_2.700603"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLH31_08235"
     CDS             complement(<1..147)
                     /locus_tag="PLH31_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HQR89237.1"
                     /translation="MTKRTRTLWGSIAAAAMISLMPVSGAMADGYWQCVPFARLMSGI
                     QIFGD"
     gene            complement(218..>574)
                     /locus_tag="PLH31_08240"
     CDS             complement(218..>574)
                     /locus_tag="PLH31_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR89238.1"
                     /translation="GAPDMIIVAALGGLILALACLAASGSSFATQSIFVYLGEISYST
                     YMICIPWKIIAVNAATKILGIEGEQLPLLIWIAIVAALVPLSAASYHLIEKPAREHMK
                     SWAQRRAQRRPAAVGA"
     assembly_gap    575..584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(585..1262)
                     /locus_tag="PLH31_08245"
                     /pseudo
     CDS             complement(585..1262)
                     /locus_tag="PLH31_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515728.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            1379..2176
                     /locus_tag="PLH31_08250"
     CDS             1379..2176
                     /locus_tag="PLH31_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQR89239.1"
                     /translation="MNELLNLAIDPAAWAALVTLIVMEVVLGIDNLVFISILSNKLPP
                     EHRQRVRRIGISLALIMRLVLLSTIAFIVGLTAPVFDLGLTGPLGSHGEPTFETALSW
                     RDLILLAGGLFLIWKATKEIHHTVDPKQESHDVLDKTGAAISNVGSAIFQIILLDLVF
                     SIDSILTAVGMTEHLPIMVVAVLVAVTVMLLAADPLANFINNNPTVVMLALGFLLMIG
                     TVLIAEAFGMHVPKGYIYTAMLFSGVVEGLNMMSRRAAEKKKAAGGD"
     gene            complement(2311..>2874)
                     /locus_tag="PLH31_08255"
     CDS             complement(2311..>2874)
                     /locus_tag="PLH31_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQR89240.1"
                     /translation="QAAAKGHNFPNLTLVGVVDADLSLRGGDLRAGERTFQLLAQAAG
                     RAGRHEKPGRAMLQTYTPDHAVLQALKAQDRDAFVEAEMRMREEAGLPPFGRLAAVIA
                     SGPDGAALDTYVEALAAVIPNAEGVEVFGPADAPLALVRGRRRKRFLVRADRSVDLQG
                     FMAAWRARARVPNSVRVVIDIDPYSFL"
BASE COUNT          532 a          923 c          949 g          460 t
ORIGIN      
        1 gtcgccgaaa atttgaatgc ccgacatcag acgtgcgaac ggaacacatt gccagtaccc
       61 gtcggccatg gcgccggaaa ccggcatcag gctgatcatg gcggcggcgg cgatggaacc
      121 ccagagggtt ctcgtccgtt tcgtcatgct gtgggtactc ccccgtgtca ctgggccaag
      181 cagtaacctt gtcagggaca tgtgaaaaga gtgtttctta ggcgccgacc gcggcgggtc
      241 gccgctgcgc ccggcgttgg gcccaagatt tcatgtgctc gcgggccggt ttctcgatca
      301 gatgatagga tgcggccgag agcggaacca gcgcggcgac aatcgcgatc cagatcagca
      361 ggggcaattg ctcgccctcg atacccagga tttttgtcgc ggcattgacc gcgatgatct
      421 tccacgggat gcagatcata taggtcgaat agctgatctc gcccagatag acgaagatgc
      481 tttgcgtggc gaagcttgaa ccgctggcgg ccagacaggc cagcgccaga atcaggccgc
      541 ccagcgccgc cacaatgatc atatccgggg cgccnnnnnn nnnngctggg gatcacgccc
      601 gaccgccaca gggcgtgcag ggcgcagcca aaggcgaaac acggcacgat gcgcagggcg
      661 ccccacttga tcgtggcggc ggtcagcgga aagccggcca gggcctggaa ggcgggatag
      721 agcgcggcga tgaaggccag ggccaggccc acggccagga agggcttctc tcgcagcttc
      781 caggcggcga aggcgaaggc ggggaaggtc agataggcga accactcggc cgagatcgac
      841 cacgaggcgt ggttccagcc cgccacgggc gcaaagcccc aggcgtgaac cagcagcaga
      901 ttggccggca gggcttccca cgagacgata ttgttgccca ccgcgatgcc ggcgaggctc
      961 gcgacggcgg ccatcaggcc tatgcccagc agggtggcca gatgcagcgg atacacccgc
     1021 gccagccggt tccacaggaa gtcgccatag cggaactggc cctggccaaa gccctgcaga
     1081 tagacgtggc acaggataaa gcccgacagg atgaagaagg cctcgacgcc cagatagccc
     1141 ttggtcgcga gcgccggtgt gaagccgacc gcgaggttgg gccagtagtg aaatatcacc
     1201 acccagaagg cggcgatgaa cctaagcgcg gttaggggct tgatattggc ggcgtctggc
     1261 ataattcagg tccggtttgg cgaagcctgg gggcttccgt cgcgttccct tgcatggacg
     1321 gggcccacgg aggggtgggt cgagacgctt ttccagacac aagcattaga acaggacgat
     1381 gaacgaactc cttaacctcg ctattgaccc ggccgcctgg gcggccctgg tcaccctgat
     1441 cgtcatggaa gtcgtgctcg gcatcgacaa cctggtgttc atttcgatcc tctcgaacaa
     1501 gctgccgccc gagcatcgtc aacgggttcg ccgcattggc atctcgctgg cgctgatcat
     1561 gcgtctggtc ctgctgtcga ccatcgcctt catcgtcggc ctgaccgcgc cggtgtttga
     1621 tctgggcctg acgggaccgc tgggctcgca tggcgaaccg accttcgaga cggccctgtc
     1681 ctggcgcgac ctgatcctgc tggccggcgg cctgttcctg atctggaagg ccaccaagga
     1741 aatccaccac acggtcgacc ccaagcagga aagccatgac gtgctggaca agaccggggc
     1801 ggcgatctcc aacgtcggct cggcgatctt ccagatcatc ctgctggacc tggtgttctc
     1861 gatcgactcg atcctgaccg ccgtgggcat gaccgagcac ctgccgatca tggtcgtcgc
     1921 cgtgctggtc gccgtgacgg tgatgctgct ggccgccgac ccgctggcca acttcatcaa
     1981 caacaacccc accgtggtga tgctggccct gggcttcctg ctgatgatcg gcaccgtgct
     2041 gatcgccgag gccttcggca tgcatgtgcc caagggctac atctatacgg ccatgctgtt
     2101 ctcgggcgtg gtcgagggcc tgaacatgat gtcgcgcaga gcggccgaga aaaagaaggc
     2161 ggcgggcgga gactgatctt ccttccccct ttgatggggg aaggtggccc caaaggggtc
     2221 ggatgggggt gatgcggccg gtcagtacgc ttggccggcg ccgtatcacc cccatccctg
     2281 gcccttcccc catcaagggg gaagggagga tcaaagaaag ctgtaggggt cgatgtcgat
     2341 gacgacccgc acggaattgg ggaccctggc ccgcgcccgc caggcggcca tgaagccctg
     2401 caggtccacc gagcggtcag cccgcaccag gaaccgcttg cgccggcgcc cgcgcaccag
     2461 ggccaggggc gcgtcggcgg ggccaaacac ctcgaccccc tcggcattgg ggatcacggc
     2521 ggccagggcc tcgacatagg tgtccagcgc cgcgccgtca gggcccgagg cgatcaccgc
     2581 cgccagccgg ccaaacggcg gcaggcccgc ctcctcgcgc atccgcatct cggcctcgac
     2641 aaaggcgtca cggtcctggg ccttcagggc ctgcagcacg gcatggtcgg gggtataggt
     2701 ctgcaacatg gcccggcccg gcttctcatg ccgccctgcc cgaccggccg cctgggccag
     2761 caactggaag gtccgctcgc cggcccgcag gtcgccgccg cgcagggaca ggtcggcgtc
     2821 cacgaccccc accagggtca ggtttgggaa attgtggccc ttggccgccg cctg
//
LOCUS       DAOMEO010000323         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3804_length_2872_cov_2.413916, whole genome shotgun sequence.
ACCESSION   DAOMEO010000323 DAOMEO010000000
VERSION     DAOMEO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3804_length_2872_cov_2.413916"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..544
                     /gene="bfr"
                     /locus_tag="PLH31_08260"
     CDS             62..544
                     /gene="bfr"
                     /locus_tag="PLH31_08260"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQR89241.1"
                     /translation="MQGDPGIIRLLNAVLTNELTAVNQYFLHARMYDNWGFKRLGKIT
                     YDESIGEMKHADKLINRILFLEGLPNLQDLHKLKIGETVPECLASDLAVEVGGRETLI
                     PGVIQCEQARDYVSRELLREILSDTEEHIDFLEMQLGLVKSIGEQNYLQSAMGELPAG
                     "
     gene            complement(549..>1335)
                     /locus_tag="PLH31_08265"
     CDS             complement(549..>1335)
                     /locus_tag="PLH31_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR89242.1"
                     /translation="LGQIRQSSGGVGSGAAEIAQAADDLSHRTEQQAASLEQTAAALD
                     QITSTVRQTADGARKANEIVADTRRHAQATETAVGAAVRAVGEIQTSSNQISQIIGVI
                     DEIAFQTNLLALNAGVEAARAGEAGRGFAVVAQEVRALAQRSADAAKEIKVLIGTAST
                     KVSEGVGLVGQTGTALGDILERVSQIAALVSEISASSQEQSTGLNEVNTAINQMDQMT
                     QQNAAMVEQTTAAAHSLRNESGRLAALVGQFNLEPATRQARAA"
     assembly_gap    1336..1345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1346..2343)
                     /locus_tag="PLH31_08270"
     CDS             complement(<1346..2343)
                     /locus_tag="PLH31_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR89243.1"
                     /translation="MNLFGASLAVGLLMLAAASWIAIETVRVGGPLYSKIVAGKDLTA
                     DILPPPLYIIDGYLDAKLTYAAAGSADLKTAARELARHRAEYDTRLAYWREIDFDPEV
                     KSVLMGESNDQAQIVWTSADAMLKAMASGDVAAAATHEAALTQGFEAHRAAVEQIVPL
                     IAADNVRIEAEAHTTMVQQMALMGGLCVLMGAIIAGGVWAMGRRVVRPVEAITRYMGD
                     LAAGDYERAVPYAGRQDELGDMAKSVAVFREGVLERRAMRQEQDDERLKSETERQSSE
                     DNRRAAEQKRDSAMRSLAQGLDHLSAGNVAYRLETAFAPEYERLRGDFNAAAERVQA"
     gene            2504..>2872
                     /locus_tag="PLH31_08275"
     CDS             2504..>2872
                     /locus_tag="PLH31_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89244.1"
                     /translation="MKRNRTPFVKRHHLSLGADYALQLIRIFTDLFDGDMDLALVFIA
                     AAQAGTEHLRAQGDYVDLLTTEFFPDDLRRPVSVSALARSLGIPVETTRRHVVKLTQT
                     GFAQRTESGGVIVTSEVLQRE"
BASE COUNT          460 a          956 c          926 g          520 t
ORIGIN      
        1 ggttgaagcc tgtcgcgcct tgcctcactg tcgcgccgac tcaattttta ggaggcgcgc
       61 catgcaaggc gatcctggca ttatccgcct gctcaacgcg gtgctcacca acgagctgac
      121 cgccgtgaac cagtatttcc tgcacgcgcg gatgtacgac aactggggct tcaagcgcct
      181 gggcaagatc acctatgacg agtcgatcgg cgagatgaag cacgccgaca agctcatcaa
      241 ccgcatcctg ttcctggaag gcctgcccaa cctgcaggac ctgcacaagc tgaagatcgg
      301 cgagaccgtt cccgaatgcc tggccagcga cctggccgtc gaggttggcg gtcgcgagac
      361 cctgatcccg ggcgtcatcc agtgcgaaca ggcccgcgac tatgtcagcc gcgaactgct
      421 gcgcgagatc ctcagcgaca ccgaggagca catcgacttc ctcgagatgc agctgggcct
      481 ggtcaagtcg atcggcgagc agaactacct gcagagcgcc atgggcgaac tgccggctgg
      541 ctgacgcctc aggccgcccg cgcctgtcgg gtcgccggct ccaggttgaa ctggcccacc
      601 agcgcggcca gccgcccgga ctcgtttcgc agcgaatgcg ccgccgccgt ggtctgctcg
      661 accatggcgg cgttttgctg agtcatctgg tccatctggt tgatcgccgt attgacctcg
      721 ttcaggccgg tcgactgttc ctgggacgag gccgagattt ccgacaccag ggcggcgatc
      781 tggctcacgc gctccaggat gtcgcccagg gcggtgccgg tctggccgac cagtccaacg
      841 ccttcgctga ccttggtcga agccgtgccg atcagcacct tgatctcctt ggcggcgtca
      901 gccgagcgct gggccagagc ccgcacttcc tgggccacga ccgcgaagcc gcgaccggcc
      961 tcgccggccc gcgccgcttc aacgccggcg ttcagggcca gcaggttggt ctggaacgca
     1021 atctcgtcga tcacgccgat gatctggctg atctggttcg aggatgtctg gatctcgccc
     1081 accgcgcgca cagccgcgcc aacggcggtt tcggtggcct gggcgtgacg ccgggtgtcg
     1141 gcgacgatct cgttggcctt gcgggccccg tcggcagtct ggcggacggt cgaggtgatc
     1201 tggtccaggg ccgcggcggt ctgttccagg ctggcggcct gttgctcggt gcggtgcgac
     1261 aggtcatcgg ccgcctgggc gatctcggcc gcgcccgatc ccacgccgcc cgaggactgg
     1321 cggatctggc ccagcnnnnn nnnnngcctg cacccgctcg gcggcggcgt tgaagtcgcc
     1381 gcgcaggcgc tcgtactccg gcgcgaacgc cgtctccagg cggtaggcga cattgcccgc
     1441 cgacaggtgg tccaggccct gggccaggct gcgcatggcg ctgtcgcgct tctgctcggc
     1501 ggcgcggcgg ttatcctcac tggactggcg ttcggtctcg gacttcaggc gttcgtcatc
     1561 ctgctcctgg cgcatcgcgc ggcgctccag aacgccctcc cggaagacgg cgacggactt
     1621 ggccatgtcg cccagctcgt cctggcgacc ggcatacggc acggcgcgct catagtcgcc
     1681 tgccgccaga tcgcccatgt aacgggtgat cgcctcgacc ggacgcacca cgcggcggcc
     1741 catggcccag acgccgcctg cgatgatggc ccccatcagc acgcagagcc cgcccatcag
     1801 ggccatttgc tgaaccatcg ttgtgtgcgc ttcggcctcg atccggacat tgtcggcggc
     1861 gatcagggga acaatctgct cgaccgccgc gcgatgcgct tcgaagccct gggtcaacgc
     1921 tgcctcgtgc gtcgccgcgg cggcgacgtc gccgctcgcc atggccttga gcatggcgtc
     1981 ggctgacgtc cagacgatct gggcctggtc attggactcg cccatcagga cggatttcac
     2041 ttcggggtcg aaatcgatct cgcgccagta ggcaaggcgc gtatcgtatt ccgcgcgatg
     2101 ccttgccagt tcccgggccg ccgtcttcag gtcggccgag ccggcggcgg cataggtcag
     2161 cttggcgtcg agatagccat cgatgatgta gagcggcggc ggcaggatgt cagcggtcag
     2221 gtccttgccc gccacgatct tgctgtagag ggggccgccc acccgcaccg tctcgatcgc
     2281 gatccaggaa gccgccgcca gcataagcag gccaacagcc aggctcgcgc cgaacaaatt
     2341 catggctccc gacaggcgca gtcgcatttc tcatgacccc gtgttttgct ggctcaggtg
     2401 cgggccgacg ggtgaacaag atcttaaaat gggtagttct tctgctcaaa atgagtagtg
     2461 cctgaggcgt taaacattaa tagatacagg cctttgtctg gaaatgaagc gcaatagaac
     2521 gccgttcgta aaacggcatc atctgagtct cggtgcggac tatgctctgc agctgattcg
     2581 gatattcacc gatctgttcg acggcgacat ggatctggcc ctggtgttca tcgcagccgc
     2641 ccaggccggc accgagcacc tgcgcgcgca gggcgattat gtggatctgc tcaccaccga
     2701 gttcttcccg gacgatttgc ggcgaccagt cagcgtctcg gccctggccc gatcgctggg
     2761 cattcccgtg gagacgaccc gccgccatgt ggtgaagctg acccagacgg ggtttgccca
     2821 gcgcacagaa tccgggggtg tgatcgtcac ctcggaggtg ctgcagcgcg ag
//
LOCUS       DAOMEO010000324         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3805_length_2869_cov_2.328003, whole genome shotgun sequence.
ACCESSION   DAOMEO010000324 DAOMEO010000000
VERSION     DAOMEO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_2869_cov_2.328003"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLH31_08280"
     CDS             <1..447
                     /locus_tag="PLH31_08280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQR89245.1"
                     /translation="NSSSISDGAAALVMTRESVARRLGLPIVARVVSHAAHAHEPGLF
                     TTAPVPAMKKALKKAGWEVSDVDLWEVNEAFAVVAMIAQQELGFSPDKLNVNGGACAL
                     GHPIGASGARILCTLISALQARGGKKGMASLCIGGGEATAMAVELA"
     gene            481..750
                     /locus_tag="PLH31_08285"
     CDS             481..750
                     /locus_tag="PLH31_08285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR89246.1"
                     /translation="MPDMFRHNPELSRYELEVDGLLAFADYQRSGHRLVIPHVETDPA
                     LRGQGAAARLMAQVAQVARSEGMRITPLCSYAAAWLKRNDPDLIA"
     gene            complement(808..1275)
                     /locus_tag="PLH31_08290"
     CDS             complement(808..1275)
                     /locus_tag="PLH31_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR89247.1"
                     /translation="MRWRFVGRRPCSVSGSTDETTREALDRATAAGKLAEGKFMYSMV
                     LSDDSVKFPVDTATLSPEAEQRLTEFAEKLKGENKNVYLEIQGHTDNVGDPAYNDNLG
                     EKRAEAVRKFLSLRGIALNRMSTISYGEEAPAVANDTRDSRAQNRRVVVMVLS"
     gene            complement(1456..>2869)
                     /locus_tag="PLH31_08295"
     CDS             complement(1456..>2869)
                     /locus_tag="PLH31_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR89248.1"
                     /translation="LIDEASSRVRMAIDSKPEELDEIDRRLVQLKIEREALSKETDAA
                     SRQRLEKLEDEIDDLQGQSDEMTARWKAEKDKVGGAAQAREALDRLRADLATAQRAGD
                     FARAGQIQYGEIPALEARLAAAESVDGAAQKSLTPEVVDAEQIAAVVSRWTGVPVEKM
                     LEGEREKLLQMELALRARVVGQDEALEAVSDAVRRARAGLQDPARPIGSFLFLGPTGV
                     GKTELTKALAEFMFADEHAITRMDMSEYMEKHSVSRLIGAPPGYVGYDEGGALTEAVR
                     RRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSEYL
                     ANQEDGEDVDAVRPQVMNVVRGHFRPEFLNRIDEIILFKRLGRANMADIVRIQLQRVE
                     KLLADRRMTLAIDAEALLWLGEKGYDPVYGARPLKRVIQKDLVDPIARRLLAGELEDG
                     GVISVGVQDGALNIGKAVLQ"
BASE COUNT          461 a          975 c          927 g          506 t
ORIGIN      
        1 aattccagct cgatcagcga cggcgccgcc gcgctggtga tgacgcgcga gagcgtggcc
       61 aggcgcctgg gcctgccgat cgtcgcccgt gttgtcagcc acgccgccca cgcccacgag
      121 cccggtctgt tcaccaccgc cccggtgccg gccatgaaga aggctctgaa gaaggctggg
      181 tgggaggtct ccgacgttga cctctgggag gtcaatgagg cgttcgccgt cgtggccatg
      241 atcgcccagc aggagctggg cttttcgccc gacaagctga acgtcaacgg cggcgcctgc
      301 gccctgggcc atccgatcgg cgcgtcgggc gcgcgcattc tctgcaccct gatctcggcc
      361 ctgcaggcgc gcggtgggaa aaagggcatg gccagcctgt gcatcggcgg cggcgaggcc
      421 acggccatgg cggtggagct ggcctagcat cctctatcgg cgatctgcgt tgagactccc
      481 atgcctgaca tgttccgaca caaccccgaa ctgagccgct acgaactgga ggttgatggc
      541 ctgctggcct tcgccgacta tcagcgtagc ggccaccgcc tggtgatccc tcacgttgag
      601 accgacccag ccctgcgcgg gcagggcgca gccgcgaggc tgatggcgca ggtcgcccag
      661 gtggcgcgat cagagggcat gcggatcacc ccgctatgct cctatgccgc cgcctggctc
      721 aagcggaacg atccagacct gatcgcctag accatcgaaa agccgctctc ccgggtgaag
      781 aagaccaggg agagcggctt tgaaggttta ggacagcacc atcaccacca cgcggcggtt
      841 ctgggcccgg ctgtcgcggg tgtcgttggc cacagccggg gcctcctcgc cgtacgagat
      901 cgtggacatg cggttgagcg cgatgccgcg caggctgagg aacttgcgca cggcctcggc
      961 gcgcttttca cccagattgt cgttataggc cggatcgccg acattgtcgg tgtgaccctg
     1021 gatctccaga tagacgttct tgttctcgcc cttcagcttc tcggcgaact cggtcagccg
     1081 ttgttcggcc tcgggcgaca gggtggcggt gtcgaccggg aacttcaccg agtcgtcgct
     1141 cagcaccatc gagtacatga acttgccctc ggccagcttg ccggcggcgg tggcgcgatc
     1201 aagggcctcg cgggtcgtct cgtccgttga tccgctgacg ctacaagggc gccgaccaac
     1261 gaaccgccag cgcatctggg cgaaaaaggg gcctgctcag ggccgctttg acgccgaact
     1321 tgaccataag aatcaacgac ttctatactc ccgcgcaagc gcggaagccg ccatctggtt
     1381 cccggaatta tttgctcaag tcaccggaga ttcccctccc ccgcgacaag cggaggaggg
     1441 gaatcgggcc agccactact gcagcacggc cttgccgata ttcagcgctc cgtcctgaac
     1501 gccgacagag atcactccgc cgtcttccag ttcaccggcc agcaggcggc gagcgatggg
     1561 gtcgaccagg tccttctgga tcacgcgctt caaggggcgc gcgccataga ccgggtcata
     1621 gcccttttcg cccagccaca gcagggcctc ggcgtcgatg gccagggtca tgcggcggtc
     1681 ggccagcagc ttctcgacgc gttgcagctg gatgcggacg atgtcggcca tgttggcccg
     1741 gcccagccgc ttgaacagga tgatctcgtc gatccggttc aggaactcag gacggaagtg
     1801 gccgcgcacc acgttcatca cctgcgggcg caccgcgtcg acatcctcgc cgtcttcctg
     1861 gttggccaga tattcggagc cgaggttgct ggtcatgatg atcagggtgt tgcggaagtc
     1921 gaccgtgcgg ccctgaccat cggtcaggcg gccatcgtcc agcacctgca gcagcacgtt
     1981 gaacacgtcc ggatgggcct tctcgacctc gtcgaacagg acgacctgat acggacgccg
     2041 acgcacggcc tcggtcaggg cgccgccctc gtcgtagccg acatagccgg gaggcgcccc
     2101 gatcaggcgg ctgaccgagt gcttttccat gtactcgctc atgtccatcc gggtgatggc
     2161 gtgctcgtcg gcgaacatga actcggccaa ggctttggtc agctcggtct tgcccacgcc
     2221 cgtgggaccc aggaacagga agctgccgat tggccgcgcg ggatcctgca gaccggccct
     2281 cgcccgccga acggcgtcag aaacggcttc cagggcctcg tcctgaccca ccacgcgggc
     2341 gcgcagggcc agttccatct gcagcagctt ttcgcgctcg ccttccagca tcttctcgac
     2401 cggcaccccg gtccagcggc tgaccacggc ggcgatctgc tcggcgtcga cgacctcggg
     2461 ggtcagcgac ttctgggccg cgccatcgac ggactcggcc gccgcaaggc gagcttccag
     2521 agcggggatc tcgccgtact ggatctggcc ggcgcgagcg aaatcgccgg cgcgctgggc
     2581 ggtggcgaga tcggcgcgca gacggtcgag ggcttcacgc gcctgcgcgg ccccgcccac
     2641 cttgtccttc tcggccttcc agcgggcggt catctcgtcg gactggccct gcaggtcgtc
     2701 gatctcgtct tccagctttt ccaggcgctg gcgcgaggcc gcgtcggttt ccttcgacag
     2761 cgcctcgcgc tcgatcttca gctgcaccag gcgacggtca atctcgtcca gctcttcagg
     2821 cttgctatcg atcgccatcc gcacgcggct ggaggcctcg tcgatcagg
//
LOCUS       DAOMEO010000325         2865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3813_length_2865_cov_2.332740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000325 DAOMEO010000000
VERSION     DAOMEO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_2865_cov_2.332740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    288..297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            696..1379
                     /gene="trbF"
                     /locus_tag="PLH31_08300"
     CDS             696..1379
                     /gene="trbF"
                     /locus_tag="PLH31_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HQR89249.1"
                     /translation="MNPFKRPNDRYGSSAPVESPYLRASREWDMRIGSAVVQAKNWRM
                     AAFGGLGLAALALGGFIYEANNTNIATYVVPIDRYARPGRIELAGRTYEPTTAEIGYF
                     LADWVRRTRSKSIDPIVIRDNWTGAYHFVSGPAIGQLNAFAKANDPFANAGTQAVNVE
                     IVSVLPRSPHTYQVQWRETQFNAGTSGVTEAWTGLFTTKVVAPKNEAELRANPLGIYI
                     TEFQWSREL"
     gene            1619..2365
                     /gene="trbG"
                     /locus_tag="PLH31_08305"
     CDS             1619..2365
                     /gene="trbG"
                     /locus_tag="PLH31_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HQR89250.1"
                     /translation="MQSANRSARDYPSAGAYVNAALYYDFEPGRLYTVHTSPRFLTAI
                     SLRPGEKLISKAAGDTVRWVLGETVQGSGATQQVIVMVKPIRGGLRTNIILTTDQRTY
                     LLDAVSYEGDTYTSVISWNYPQEEARERQAAQAAESQGVAASGLAVDALHFGYRIKAN
                     GFRAPRWQPVRVFDDGLKTYIQFPRDMATTEAPPLFLIGPDKQAQLVNYRYLGGYYVV
                     DRLIDVAELRLGEGKQTVVRITRTGEGGRS"
     gene            2362..>2865
                     /locus_tag="PLH31_08310"
     CDS             2362..>2865
                     /locus_tag="PLH31_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HQR89251.1"
                     /translation="MTHLPPDPPLNPHPRKAAPESVLAAPRNPVTRWNRKYLIAGATG
                     LAGIVALGFYLGFGGAHAPQARPEAMTDAADTGQVQTPAISTRYAAGYADPALQAQAL
                     PGTATLPPPEGALGTLPAGTPPPPPPPAQVDPAVQAAREQALAARTSGPFFGGPPSAA
                     PPAPPADL"
BASE COUNT          455 a         1040 c          929 g          431 t
ORIGIN      
        1 cttttggtgg gcggactcta attattcctg gaggagtttc agggggtcac gtcatcgcgg
       61 gcgtggggat caacatcttc gagacctaca ctttatcgcc tcagccgacc gtggcggagg
      121 aagccggcct gctcttgtcg gcgatcttcc tgatgatgct agcgctcaaa gttcccgcga
      181 tcgccggcgc catgatttca ggtggcccgc agctgaatac gggtggcgct ctgatgggcg
      241 cggcgggtgt tgccgccggc gcagcgggcg taggcctcgc cgcccgcnnn nnnnnnnaag
      301 atcgcggcgg ccagggccgc ttcagggagc atcaattcag gtggcccgcg tcccacaggt
      361 ggcgcttctg gcggcggcgg catggcggcg gacttcgcca gggcacccgt ctcgacgacg
      421 gtcgccgaag ggcgagcggc cggcagtcgc ctcttcgcct cccgcaatcc cagtgaaggc
      481 gagacgcccc ctccccctcc gccagaagcg ccctcgtctg gatcctccgt cgtttcgcgg
      541 gccgaggctc gccgtggcgg cgggctcgca ggcgcggctc gcgacagcgc tgctgcggcg
      601 accgccggcg gcgacggaag cggcccgggc ctgagcgcca cgccaacccg ccgcgatgac
      661 gaccctgaag agtgacccca gtcgagagtg cgccgatgaa ccccttcaaa cgaccgaacg
      721 accgctacgg aagctccgcc ccggtcgaga gcccctacct tcgtgcctcc cgggagtggg
      781 atatgcggat cggctcagcc gttgtgcagg ccaagaactg gcggatggcg gcgttcggcg
      841 ggttggggct cgcggccctg gcgctgggcg gcttcatcta tgaggccaac aacaccaaca
      901 tcgccaccta cgtcgtgcca atcgaccgct acgcgcggcc tggccggatc gagctggccg
      961 ggcggaccta tgagccgacc acggccgaga tcggctactt cctcgcggac tgggtgcgac
     1021 ggacccgctc taagagcatc gatcccatcg tcatccggga caactggacc ggggcctatc
     1081 acttcgtgtc agggccagcg attgggcagc tcaacgcctt cgccaaggcc aacgacccct
     1141 ttgccaatgc gggcacccag gcagtcaacg tcgagatcgt gtcggtcctg ccgcgcagcc
     1201 cgcacaccta ccaggtccaa tggcgcgaga cgcagttcaa tgccgggacc tccggagtga
     1261 ccgaagcctg gaccggcctg ttcaccacca aggtggtggc gccgaagaac gaggcggaac
     1321 tgagggccaa cccgctggga atctacatca ccgagtttca atggagtcgt gagctatgag
     1381 cgccttgtcc cccagacggc tttccatggg cgctgcggca ggcctgctcg tcgtgctcgc
     1441 cgcgtctgaa agtccgtccg tcgcccagac ggccgcgcca cggcccgctc ccccgccagc
     1501 tgttcggtcg acgcccccgg cgcctgtggc tgtggcccag gccgggacgc cgcctcggcc
     1561 aacgccgaga cgcagaacgg cgccgcgcac ctacgcgcgc ccccaagcct tgacgaccgt
     1621 gcagtccgcc aaccgcagtg cgcgcgacta cccgagcgcc ggcgcctatg tgaacgcggc
     1681 cctctactac gacttcgagc ctgggcggct ttataccgtc cacaccagcc cgcgcttcct
     1741 gaccgcgatc tccctgcgcc cgggcgaaaa gctaatctcc aaggcggcgg gcgacactgt
     1801 gcgctgggtc ctcggcgaga cggttcaggg ctcgggcgcg acccaacagg tcatcgtgat
     1861 ggtcaagccg atccgcgggg gcttgcgcac aaacatcatc ctgacgaccg accagcggac
     1921 ctacctgctc gacgccgtca gctacgaggg cgacacctac accagcgtca tcagctggaa
     1981 ctatccgcag gaagaggccc gcgagcggca ggccgcccaa gctgccgagt cgcaaggcgt
     2041 cgcggcttct ggcctggcgg tggacgcact gcacttcggt tatcgcatca aggccaacgg
     2101 ctttcgcgca ccgcgctggc agccggtccg cgtcttcgac gacggcctga agacctacat
     2161 ccagtttccg cgcgacatgg ccacaaccga ggccccgcca cttttcctca tcggtcctga
     2221 caagcaggca cagctcgtca actaccgcta tctcggcggc tactatgtcg tggaccgcct
     2281 gatcgacgtc gcggagctgc ggctcggcga aggcaagcag accgtggtgc ggatcacccg
     2341 caccggcgaa ggggggcgct cgtgacccac cttccgcccg atccccctct caaccctcac
     2401 ccgcgcaagg ccgcgccgga aagcgtcctg gccgcgccac gaaatccagt cacgcgctgg
     2461 aatcgcaagt acctgatcgc cggggcgacg ggtctcgccg ggatcgtggc gctaggcttc
     2521 tatctgggct ttggcggcgc ccacgcgccc caggcccgcc cggaggcgat gacggatgcg
     2581 gcggacaccg gccaggtcca gacgccggcg atctccacca gatatgcggc gggctacgcc
     2641 gatccggcgc tgcaggcgca agccctgcct gggaccgcga ccttgcctcc gccggaaggg
     2701 gcgctcggta cgctgccggc gggaacgccg cccccgccgc cgcctccggc gcaagttgat
     2761 cctgccgttc aggctgcccg cgagcaggcg ctggccgcgc ggacctccgg cccctttttc
     2821 ggcggtccgc cgtcagcggc gcctccggcg ccgcccgccg atctc
//
LOCUS       DAOMEO010000326         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3817_length_2862_cov_2.772355, whole genome shotgun sequence.
ACCESSION   DAOMEO010000326 DAOMEO010000000
VERSION     DAOMEO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3817_length_2862_cov_2.772355"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            818..2320
                     /locus_tag="PLH31_08315"
     CDS             818..2320
                     /locus_tag="PLH31_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR89252.1"
                     /translation="MAQHVETLVIGAGPAGLTTAYTLAKAGRGVTVLEMDPVYVGGIS
                     RTVDYKGFKFDIGGHRFFSKSKEVVDLWNEILPDDFIDRPRLSRIYYREKFYAYPLKA
                     FEALANLGIWTAARCMASFGWAKARPIKDPTTFHQWVRNQFGEKLFSIFFKTYTEKVW
                     GMSCDEISADWASQRIKGLDLAAAIVDGVKRSLGVRAKAGAEGAPKTLIESFRYPRKG
                     PGMMWEACADKILALGGQLRMGRKVDGLYYDKIAKIWTVSVTRADGTVEIYTADDVVS
                     SAPLRELMGAVSPTPISLFHAGELMYRDFITVVLIGKPQKELPDNWIYIHDPSVKVGR
                     VQNFRSWSPEMIPDGVSTCLGLEYFCFEGDGLWTSTDEDLVAQAKREIAKIGLMNPDE
                     VYDACVVRQHKAYPVYDDAYAEHIKMIRLDLKLHFPGLHLVGRNGMHKYNNQDHAMMT
                     GLLTAENIIAGETIHNVWDVNEDAEYGEAGVSGAREALSSVRAVPQKVAA"
     gene            2323..2751
                     /locus_tag="PLH31_08320"
     CDS             2323..2751
                     /locus_tag="PLH31_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQR89253.1"
                     /translation="MNLPADLWRRLPVALRELGLYGLASAAALAVDWGLLLVLHGAGL
                     NYLIAAAIGFGAGIAVTYLLSVSVVFRHRPIADRRREFAGFLGVGLAGLVLTQLLMAL
                     WVERIGLAPGLAKIPTAGLVFVFNFTVRRALLFRAPRASA"
BASE COUNT          498 a          953 c          910 g          501 t
ORIGIN      
        1 gacgggccgt gacggtcgag cgcttgcatt acaacgccga tggcacgctc aagcccgtcg
       61 tgcagacaga ggccggcgtc acggcgccga caccctagcc acctcaaccc cgcaggatct
      121 tccccttcgc cgaccggtag gactacagtt tgtggagcga gaagggggtg ggcaaggtcg
      181 tatccccgtt cttttcagaa caaaccgcaa tccgatcagc agcttgcaaa aatcttcatc
      241 gcgccagtaa gacgcgccga aaccgcccgc atactgatct cactccaagg caagggaagg
      301 gacgtcggcc ggcgacctga tgggccctgg ggtgtggtcg agcgggaagc gtccgccgtg
      361 gtcttcaatg acagccttga gcgtctgact gggcgggatc cctgaaagat cgggacgaaa
      421 acccagcggc tcgggtcgga aggcgcagca ggcatgggct gaaatcgccc atgcggtccg
      481 gaaggggtta gccgcccctc cgcccgtcga gggcctagct accggagcgg gttaaaaccc
      541 cgccaatccc ggcgatgctc tcgaataacg gctgcgcgga gcgtctgtct tcgtcgaaac
      601 ggtattcact acacactcat ccgggcgagg cccggacgct ccgcgaccct tcccattccc
      661 ttcagcggcc agccgcgtga agcgggcaag ccgtgtcctg gcctctccgc gacccatcgt
      721 cccagattca cgattaatgg cgattcacca cgcctcgcga cccgacctta accaagtctc
      781 ccccatcctg accttgcaca acgggattgg aacggacatg gcccagcacg tcgagaccct
      841 ggtgatcgga gccggccctg cgggcctgac gaccgcctat accctggcca aggccgggcg
      901 gggcgtgacc gtgctggaaa tggacccggt ctatgtgggc ggcatcagcc gcaccgtcga
      961 ctacaagggc ttcaagttcg acatcggcgg ccaccggttc ttctcgaagt ccaaagaggt
     1021 ggttgatctc tggaacgaga tcctgcccga cgacttcatc gaccggccgc gcctgtcgcg
     1081 catctattac cgcgagaagt tctatgccta tccgctgaag gccttcgagg ccctggccaa
     1141 tctggggatc tggacggccg cgcgctgcat ggcctcgttc ggctgggcca aggcccgtcc
     1201 gatcaaggac ccgaccacct tccaccagtg ggtgcgcaac cagttcggcg aaaagctgtt
     1261 ctcgatcttc ttcaagacct acaccgagaa ggtatggggc atgagctgcg acgagatctc
     1321 ggccgactgg gccagccagc gcatcaaggg cctggacctg gccgccgcca tcgtcgacgg
     1381 cgtcaagcgc tcgctgggcg ttcgcgccaa ggccggcgct gaaggcgcgc ccaagaccct
     1441 gatcgaaagc ttccgctatc cccgcaaggg ccccggcatg atgtgggagg cctgcgccga
     1501 caagatcctg gccctgggcg gccagctgcg catgggccgc aaggtcgatg gtctgtacta
     1561 tgacaagatc gccaagatct ggaccgtttc ggtgacccgc gccgatggga cggtcgagat
     1621 ctacaccgcc gacgacgtgg tctcctcggc cccgctgcgc gagctgatgg gagccgtgtc
     1681 gcccacgccg atcagcctgt tccacgctgg cgagctgatg taccgcgact tcatcaccgt
     1741 cgttttgatc ggcaagccgc agaaggaact gccggacaac tggatctaca tccacgatcc
     1801 ctcggtgaag gtgggccggg tgcagaactt ccgctcgtgg tcgcccgaga tgatcccgga
     1861 tggcgtctcg acctgtctgg gcctcgaata tttctgcttt gagggcgacg gcctgtggac
     1921 ctcgaccgac gaggacctgg tggcccaggc caagcgcgaa atcgccaaga tcggcctgat
     1981 gaaccccgac gaggtctatg acgcctgcgt cgtgcgccag cacaaggctt accccgttta
     2041 tgacgacgcc tatgccgagc acatcaagat gatccgcctg gacctgaagc tgcacttccc
     2101 gggcctgcat ctcgtcgggc gcaacggcat gcacaagtac aacaaccagg accacgccat
     2161 gatgaccggc ctgctgacgg ccgagaacat catcgccggc gagacgatcc acaatgtctg
     2221 ggacgtcaac gaggacgccg aatatggcga ggccggcgtc tcgggggctc gcgaggccct
     2281 gagtagcgtc cgcgccgtgc cgcagaaggt cgccgcctga gcatgaacct gccggcggat
     2341 ctctggcgcc gcctgccggt ggccttgcgc gaactgggcc tctacggcct ggccagcgcg
     2401 gccgccctgg cggtggactg gggtctgctg ctggtgctgc atggggcggg gctgaactat
     2461 ctgatcgccg cggcgatcgg ctttggcgcc ggcatcgcgg tgacctatct cctgagcgtt
     2521 tcggtggttt ttcgccatcg acccatcgcc gaccggcggc gggaatttgc cggtttcctg
     2581 ggcgtgggcc tcgccgggct tgtcctgacc cagctgctga tggccctgtg ggtcgagcgg
     2641 atcggcctgg cgccggggct ggccaagatc ccgacggcgg gactggtctt cgtcttcaac
     2701 ttcaccgtcc gccgggcgct gctgttccgc gcccctcgcg cttccgcgtg aacgggcaag
     2761 cgacctccca gatcggccgc tgggcgttcc tggcggtcgc cgcgtccctg atggcgctca
     2821 gccgcgtgca tctgggcctg cgtcatgacg cccggctcta tt
//
LOCUS       DAOMEO010000327         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3826_length_2859_cov_2.463267, whole genome shotgun sequence.
ACCESSION   DAOMEO010000327 DAOMEO010000000
VERSION     DAOMEO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_2859_cov_2.463267"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLH31_08325"
     CDS             complement(<1..696)
                     /locus_tag="PLH31_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQR89254.1"
                     /translation="MAISKEQIFAVADELDAAGQNPTLANVRKQLGSGSFTTISEAMN
                     EWRARKASQAAPIREPAPQAITDKLAELGGDLWAVALEMANNRLAAEREALEAVRQET
                     EAARQEAAELADQLTGELDEAKARVAALEAVEAAAKGEADELRGKLAATSERAATAEA
                     RAGELRTELDHAHQEARQARAERDKAQERATASADQVEALRADLAAANSRTAEIERRA
                     GELRADLERANQAT"
     gene            complement(876..>1662)
                     /locus_tag="PLH31_08330"
     CDS             complement(876..>1662)
                     /locus_tag="PLH31_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQR89255.1"
                     /translation="ISVRENPDAPGRYLINHGARRYRGSKWAHKTTIPAFIDNDYNEA
                     DQVIENLQRNELTAREIADFIGRELAKGKKKGEIAKEIGKSPAFVTQHVTLLDLPEPI
                     AEAFNSGRGKDVTVINELVTAYKKNPDEVAAWLADDSQELTRGSVKLLREFLEDKRSH
                     EDGDRDPNTVDALTGKTDAEAGDGEQGPQDDDAKGKKEPKEADPDKLKKAIIQVKHDD
                     RPARLILNRRPPAEGWAWLKYEDDGQEFEADLGTVQLVALLEG"
     assembly_gap    1663..1672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1673..1933)
                     /locus_tag="PLH31_08335"
     CDS             complement(<1673..1933)
                     /locus_tag="PLH31_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR89256.1"
                     /translation="MSAKTNAKKKEQDKPQSSGLGLDGLGDLAGLLNEQPAANAGGAG
                     PQELPLDLIDEDPHQPRTADNPGFSPESIAEIGETIKARGVKS"
     gene            complement(1930..2694)
                     /locus_tag="PLH31_08340"
     CDS             complement(1930..2694)
                     /locus_tag="PLH31_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR89257.1"
                     /translation="MKTLVTAIQKGGQGKTFATCHLAFDFLERGLRVAVIDLDTQGNA
                     SFTLSAYQSGYLASQLFTGDTDDLRYWFGKREGESLALIAADANLANLDKMELSQAAA
                     ALRASVAALGEFFDVCLIDTAPSLGVAMTAAVLTADYMLSPIEMEAYSLQGMKKMVAI
                     IGNLRKQNPKLRFLGMVPNKVDARKPRHVNNLATLQQAYPQLILPFSIGARDSIAEAL
                     GEQMPVWKIKKTAARKATQEVRALADYVFTKMEIAQ"
     gene            complement(2691..2843)
                     /locus_tag="PLH31_08345"
     CDS             complement(2691..2843)
                     /locus_tag="PLH31_08345"
                     /inference="COORDINATES: protein motif:HMM:NF027824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrfB-related DNA-binding protein"
                     /protein_id="HQR89258.1"
                     /translation="MSQAVSRVWAAAGEQLPEGFERVTAVLPEHQAFIVKKWEADAKR
                     KQEPKS"
BASE COUNT          339 a          987 c          929 g          594 t
ORIGIN      
        1 ggtcgcctgg ttcgcccgct ccaggtcggc acgcagctcg ccggcgcggc gctcgatctc
       61 ggcggtacgg ctgttcgcgg ccgcgaggtc ggcccgcagg gcctccacct ggtcggcgct
      121 ggccgtggcc ctctcctggg ccttgtcgcg ctctgcgcgc gcctggcggg cttcctgatg
      181 ggcgtgatcc agctccgtgc gcagctcgcc ggccctggcc tcggccgtgg ccgcgcgctc
      241 gctggtcgcc gcgagcttgc cgcgcagctc gtcggcctcg cccttggcgg ccgcctcgac
      301 ggcctccagg gccgcgacgc gggccttggc ctcgtccagc tcgccggtga gctggtcggc
      361 cagctctgcg gcttcctggc gcgctgcttc cgtctcctgg cgcacggcct ccagcgcctc
      421 gcgctcggcg gccaggcggt tgttcgccat ttccagcgcc acggcccaca agtcgccgcc
      481 caactcggcc agcttgtcgg tgatcgcctg cggtgctggc tcgcggatcg gggcggcctg
      541 gctggccttg cgtgcgcgcc actcgttcat cgcctcgctg atggtggtga agctgccgct
      601 gccgagctgc ttgcgcacgt tcgccagcgt ggggttctgg ccggccgcgt ccagttcgtc
      661 ggccaccgca aatatctgct ccttcgagat cgccatgtcg ggcctccttt ggccggttgt
      721 atgttgtagc gtgtagcata ctacaacata caacactacg caagcggttt agctgaactt
      781 tttttagcgg ctaaaacgcg ggcaaaaaaa agccccgctc gatggcgggg cgatgggtgg
      841 gggtcggtgg tcggcgatgc ccggcgctgg gcgtgtcagc cctccagcag cgcgacaagc
      901 tgcacggtgc caaggtcggc ctcgaactcc tggccgtcat cctcgtattt gagccaggcc
      961 cagccctcgg ccgggggccg gcggttgagg atcagccgtg ccggccggtc gtcgtgcttg
     1021 acctgtatga tggccttctt gagcttgtcg gggtcggcct ccttcggctc cttcttcccc
     1081 ttggcgtcat catcctgcgg cccctgctcg ccgtcgccgg cctcggcgtc ggtcttgccg
     1141 gtcagcgcat cgacggtgtt gggatcacgg tcgccgtcct cgtgactgcg cttgtcctcc
     1201 aggaactcgc gcagcagctt caccgagccg cgcgtcagct cctggctgtc gtcggccagc
     1261 caggcggcca cctcgtcggg gttcttcttg taggccgtca ccagctcgtt gatgacagtc
     1321 acgtccttgc cccggccgct attgaacgcc tcggcaatgg gttcgggcaa gtccagcagc
     1381 gtgacgtgct gcgtgacgaa ggccggcgac ttgccgatct ccttggcgat ctcgcccttc
     1441 ttcttgccct tcgccagctc gcggccgatg aagtcggcga tctcacgcgc cgtcagctcg
     1501 ttgcgttgca ggttctcgat aacctggtcg gcctcgttgt agtcgttgtc gatgaaggcc
     1561 gggatcgtgg tcttgtgcgc ccacttcgag ccgcgatagc gccgcgcgcc gtggttgatg
     1621 aggtagcgcc ccggtgcgtc cgggttctcg cgcacgctga tgnnnnnnnn nncgacttca
     1681 cgccgcgcgc tttgatggtc tcgccgatct ccgcgatgct ctccggcgag aagccggggt
     1741 tgtcggccgt gcgcggctgg tgtgggtctt cgtcgatcag gtcaagcggc agctcctgcg
     1801 ggcctgcgcc gccggcgttg gccgccggct gctcgttcag caggccggcc aggtcgccca
     1861 gcccgtccag gcccagcccg gacgattgcg gcttgtcctg ctccttcttc ttggcgttgg
     1921 tcttggcgct cattgcgcga tctccatctt cgtgaacacg taatccgcca gcgcacgcac
     1981 ctcctgggtg gccttccgcg cggcggtctt cttgatcttc cacaccggca tctgctcgcc
     2041 cagcgcctcg gcgatgctgt cacgcgcgcc gatgctgaac ggcaagatga gctgcgggta
     2101 tgcctgctgc aacgtcgcca ggttgttgac gtggcgcggc ttccgcgcgt ccaccttgtt
     2161 cggcaccatg ccgaggaagc gcagcttggg gttctgcttg cgcaggttgc cgatgatggc
     2221 aaccatcttc ttcatgccct gcaagctgta cgcctccatt tcgatgggcg acagcatgta
     2281 gtcggccgtc agcacggccg ccgtcatggc gacgccaagg gagggggccg tgtcgatcag
     2341 gcacacgtcg aagaactcgc ccagcgcggc cacactggcc cgcagcgcgg cggccgcctg
     2401 ggaaagctcc atcttgtcca ggttcgccag gttggcgtct gccgcgatca gcgccaggct
     2461 ctcgccctcg cgcttgccga accaataccg caggtcatcg gtgtcgccgg tgaacaactg
     2521 gctggcgagg tagcccgatt ggtacgccga cagcgtgaac gatgcgttgc cctgggtgtc
     2581 caggtcgatc acggccacgc gaaggccgcg ctccaggaag tcgaatgcca ggtggcaggt
     2641 ggcgaacgtc ttgccttgac cgcccttctg aattgccgtg accagtgttt tcatgacttg
     2701 ggttcctgtt tcctcttggc gtctgcttcc cacttcttga cgatgaacgc ttgatgctcc
     2761 ggcagtaccg ccgtcactcg ctcgaagccc tcggggagct gttcccctgc tgctgcccat
     2821 acgcgactga ccgcctgcga caccgccccc ttggtcagc
//
LOCUS       DAOMEO010000328         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3844_length_2852_cov_2.305685, whole genome shotgun sequence.
ACCESSION   DAOMEO010000328 DAOMEO010000000
VERSION     DAOMEO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_2852_cov_2.305685"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="rfaE2"
                     /locus_tag="PLH31_08350"
     CDS             <1..960
                     /gene="rfaE2"
                     /locus_tag="PLH31_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQR89259.1"
                     /translation="RQAIDAARAAGKPVIVDPKSRNLARYDGATLVKPNRKEAAEATG
                     VIDDSDEASEEAGAAILAMAPGLDAALITRGGAGMTLSARGEPPVHLPATAVEVFDVS
                     GAGDTVAATLALAVAAGASLADAARLANLAAGLVVAKLGTDVVTAAELTALARSEAGQ
                     PAEVKIADLPHALEIVAGWRARGQTVGFTNGCFDLLHPGHVSLLDQSRAACDRLIVGL
                     NTDASVSRLKGPTRPVQKEHARAVVLASLSSVDLVVLFDEDTPLNLIKAFHPDVLVKG
                     ADYTVETVVGSDVVLGYGGKVLLADLKQGQSTTALIGRMNAKT"
     gene            1080..>1451
                     /locus_tag="PLH31_08355"
     CDS             1080..>1451
                     /locus_tag="PLH31_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR89260.1"
                     /translation="MSVDRTLYHFPLDPASRQVRLALGEKRLAFAEVQVRYWEGAPDF
                     LTLNPSGLTPVLVEIRNERKTVVCEVRAILEHIEEQEPEPALLGRDAGERAEARRLLQ
                     WFDRKFDNEVNGFLLHEKMEKR"
     assembly_gap    1452..1461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1462..1762
                     /locus_tag="PLH31_08360"
     CDS             <1462..1762
                     /locus_tag="PLH31_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR89261.1"
                     /translation="APDLSSLRRGREALRHHLAYVDGLLQTRDWLAGRRMSLADFAAA
                     AHLSVIDYFGDVPWQDFQAAKTWYMKLKSRPCFRPILADRWPGLAPAAHYDDLDF"
     gene            1743..>2105
                     /locus_tag="PLH31_08365"
     CDS             1743..>2105
                     /locus_tag="PLH31_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HQR89262.1"
                     /translation="MTTSTSSHPNIAPKDEIRAEALRLGFDACGFADAAAAWPNGAWL
                     ANFVAEGFHGEMGWMEETLERRRHPTAMWTDARSAVVLGMNYGPDHDPLTALEHRDRA
                     AISVYAQGDDYHDVVKKRL"
     assembly_gap    2106..2115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2116..2849
                     /gene="queG"
                     /locus_tag="PLH31_08370"
     CDS             <2116..2849
                     /gene="queG"
                     /locus_tag="PLH31_08370"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQR89263.1"
                     /translation="RFGEEVKVFVDTAPLMEKPLAQRAGLGWQGKHTNLVSRPFGSWL
                     FLGSVLTTLDLPPDAPEVDHCGSCRACLDICPTNAFPEPNRLDARRCISYLTIELAGP
                     IPVEFRSAMGNRIYGCDDCLAVCPWNKFARVSSEAKFHARESLKGPSLAELASLDDPS
                     FRALFSKSPVKRIGRDRFVRNVLYAIGNSGDSGLMGVVEPLLSDPAPVVRGAAVWAAR
                     RLAPDRAAALRGVEDNPQVLAEWGA"
BASE COUNT          439 a          985 c          952 g          456 t
ORIGIN      
        1 cgccaggcca tcgacgcggc ccgcgccgcc ggcaagccgg tgatcgtcga tcccaagagc
       61 cgcaacctgg cccgctatga cggggccacc ctggtcaagc ccaaccgcaa ggaagcggcc
      121 gaggccaccg gcgtgatcga cgacagcgac gaagcctccg aagaggccgg cgcggcgatc
      181 ctggccatgg cgcccggcct ggacgcggcc ctgatcaccc ggggcggagc cggcatgacc
      241 ctgagcgcgc gcggcgagcc gcccgtgcac ctgccggcca cggccgtgga agtgttcgac
      301 gtctctggcg ccggcgacac cgtcgccgcc accctggccc tggccgtcgc ggccggcgcc
      361 tctctggcgg acgcggcccg cctggccaat ctggccgccg gccttgtagt ggccaagctg
      421 ggcaccgacg tggtcaccgc cgccgaactg acggctctgg cccgctccga ggcgggtcag
      481 ccggccgagg tcaagatcgc cgatctgccc cacgccctgg aaatcgtcgc cggctggcgc
      541 gcccgcggcc agactgtggg cttcaccaac ggctgtttcg acctgctcca ccccggccac
      601 gtctcgctgc tggatcagtc cagggccgcc tgcgaccggc tgatcgtggg tctcaatacc
      661 gacgcctcgg tctccaggct gaagggcccg acacggcccg tgcagaagga gcatgcccgc
      721 gccgtcgtcc tggcctcgct ctcgtcggtg gatctggtcg tgctgtttga cgaagacacc
      781 ccgctgaacc tgatcaaggc cttccacccc gacgtgctgg tcaagggcgc ggactacacg
      841 gtcgagaccg tggtcggctc tgacgtcgtg ctgggctatg gcggcaaggt gctgctggcc
      901 gacctcaagc agggccagag caccaccgcc ctgatcggcc ggatgaacgc caaaacctga
      961 acgttgcgac gacactcgcc acgcaaaggc ctgcattgtc tgggttaacg gcgttcagca
     1021 acaggttgtt aagcaaaccg gcgcatcgct ctttcgatgc agcctgagcg caagtgggca
     1081 tgagcgtcga tcgcaccctt taccatttcc ccctggaccc cgcctcgcgg caggtgcgtc
     1141 tggcgctggg tgaaaagcgt ctggccttcg ccgaggttca ggtccgctat tgggaaggcg
     1201 cgcccgactt tctcacgctc aatccctcag gcctgacgcc ggtactggtc gagatccgca
     1261 acgagcgcaa gacggtagtc tgcgaggtcc gcgccatcct cgaacatatt gaggagcagg
     1321 agcccgaacc cgccctgctg ggccgcgacg ccggcgagcg cgcggaggcc cgccgcctgc
     1381 tgcaatggtt cgaccgcaag ttcgacaacg aggtcaacgg cttcctgctg cacgagaaga
     1441 tggaaaagcg cnnnnnnnnn ncgcgcccga cctgtcttcg ctgcgccggg gccgcgaggc
     1501 ccttcgccac catctggcct atgtggatgg cctgttgcag acgcgcgact ggctggccgg
     1561 ccgccgcatg agcctggccg atttcgccgc cgccgcccac ctgtcggtga tcgactattt
     1621 cggcgatgtg ccctggcagg acttccaggc ggccaagacc tggtacatga agctcaaatc
     1681 gaggccctgc ttccggccga tcctggccga tcgctggccc ggcctcgcgc cggccgcgca
     1741 ttatgacgac ctcgacttct agtcacccga acatcgcccc aaaggacgag atccgcgccg
     1801 aggccttacg gctgggcttc gacgcctgcg ggttcgccga cgcggccgcc gcctggccca
     1861 atggcgcctg gctggcaaac ttcgtggccg aaggtttcca cggcgagatg ggctggatgg
     1921 aagagacgct ggagcgtcgc cgccatccca ccgccatgtg gaccgacgcc cgcagcgccg
     1981 tcgtgctggg catgaactat ggccccgacc acgatccgct gaccgcgctg gagcatcggg
     2041 atcgcgcggc gatctcggtc tatgcccaag gcgacgacta tcatgacgtg gtcaagaaac
     2101 ggctcnnnnn nnnnnggcgg tttggcgaag aggtgaaggt gttcgttgat accgcgccgc
     2161 tgatggaaaa gccgctggcc cagcgcgccg gcctgggttg gcagggcaag cataccaacc
     2221 tggtctcgcg gccgttcggc tcgtggctgt ttctgggctc ggtgctgacg acgctcgacc
     2281 tgccccccga cgcgcccgag gtcgatcact gcggctcgtg ccgggcgtgt ctcgatatct
     2341 gcccgaccaa cgccttccct gagcccaatc gcctcgatgc ccggcgctgc atctcctacc
     2401 tgaccatcga gctggccggc ccgatcccgg tcgagttccg gtccgccatg ggcaaccgca
     2461 tctatggctg cgacgactgc ctggcggtct gtccgtggaa caagttcgcc cgggtttcca
     2521 gcgaggcgaa gttccatgcg cgggagagcc tgaaggggcc gtctctggcg gagttggcca
     2581 gcctggacga tccgtcgttc cgggcgctgt tctccaagag cccggtcaag cggatcggcc
     2641 gcgaccggtt cgtgcgcaat gtgctctacg ccatcggcaa cagcggcgat tcaggcctga
     2701 tgggcgtggt cgagccgttg ctgagcgatc cggcgccggt ggtgcgcggg gcggcggtct
     2761 gggcggcgcg gcggttggcg ccggacaggg cggcggcgct acggggcgtc gaagacaatc
     2821 cgcaggtgct ggcggagtgg ggcgcttaac aa
//
LOCUS       DAOMEO010000329         2851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3845_length_2851_cov_2.402361, whole genome shotgun sequence.
ACCESSION   DAOMEO010000329 DAOMEO010000000
VERSION     DAOMEO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_2851_cov_2.402361"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLH31_08375"
     CDS             complement(<1..230)
                     /locus_tag="PLH31_08375"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518382.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HQR89264.1"
                     /translation="MTLSPLPDLLVRPIIDMALAEDLGRAGDITAQACIDAEARLSVV
                     FAARQDGRVAGLSCARLALAAMDPTATFQVVTP"
     gene            complement(227..820)
                     /locus_tag="PLH31_08380"
     CDS             complement(227..820)
                     /locus_tag="PLH31_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HQR89265.1"
                     /translation="MTTMNAGFEERGDSEFLFDFEQFELMDQAGVFSDVQGRVELIEG
                     KIIQMAPASIDHGGANADVVFAIESALRALSPRPALKVIVTATLRIDRHSAPLPDVFV
                     MRKSKGEKYAQAEQSVLVVEISVSTRGSDLTVKSRLYAQAGIPEYWVVEPKARKVSVF
                     REPQPDGTWALATILENDDAISPLFAPQIRIPLSELF"
     gene            complement(850..2376)
                     /locus_tag="PLH31_08385"
     CDS             complement(850..2376)
                     /locus_tag="PLH31_08385"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQR89266.1"
                     /translation="MTDRVIFDGPVILGAGLAGLTAALSATKWAAKRALVLSPTPLAT
                     GCSSAWAQGGMAAALSDDDAPALHADDTIAAGAGLCDPEAVDLLTREGPDAVRLLAAL
                     GAPFDTQDGAFTLSLEAAHSKARVARVGGDGAGAAIMAAVIAAVRAAPAIEIRETARA
                     RRLLQDATGRVLGVLAEIDGQLVEILSPSVILATGGVGGLYAVTTTPAEVRGEGLGLA
                     ALAGAVIADPEFVQFHPTAIDIGRDPAPLATEALRGEGAILRNTDGRAFMADYHPAAE
                     LAPRDIVARAIHAERAAGRGAFLDATTAVGAHFPHEFPAVFEACRSGGIDPRREMIPV
                     TPAVHYHMGGVATDLDGATSLPGLYAAGECAATGVHGANRLASNSLLEAAVFGARAGR
                     AAAMEAHAPGLPVSLEPTPDLPADVLQTLRKAMSRDAGVIRDAAGLTRLLDQIDALEP
                     AYGPCPPLVAARLIVAAALAREESRGGHFRADFPATAAPQRTFVTFDGREPSLQYAAE
                     "
     gene            complement(2534..>2851)
                     /gene="nadA"
                     /locus_tag="PLH31_08390"
     CDS             complement(2534..>2851)
                     /gene="nadA"
                     /locus_tag="PLH31_08390"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQR89267.1"
                     /translation="NDYVAARKPAQVVLITECSMASNVQAESPATQFIGPCNMCPHMK
                     RITLQNIHDALVHEQFEVTVDPVIADRARLAVQRMIDLPPPLVPARYDLVKARHHVDV
                     EMI"
BASE COUNT          441 a          924 c         1059 g          427 t
ORIGIN      
        1 ggcgtgacga cctggaaggt ggcggtcggg tccatcgccg ccagcgccag ccgcgcgcag
       61 gacaggcccg ccacccggcc atcctgacgg gcggcaaata ccaccgacag ccgggcttcg
      121 gcgtcgatac aggcctgagc agtgatgtcg ccggcccggc cgaggtcttc ggccagggcc
      181 atgtcgatga tcgggcggac gaggaggtcg ggcagggggg aaagcgtcaa aacaactccg
      241 acagcgggat acggatctgc ggggcgaaca gcggcgagat ggcgtcgtcg ttttccagga
      301 tggtcgccaa ggcccaggtc ccatctggtt ggggttctcg aaagacgctg accttgcggg
      361 ctttgggctc cacgacccag tattccggta tgccagcttg agcatagagg cgactcttga
      421 ccgtcaggtc gctgccgcgc gtggagaccg aaatctccac caccaacacc gactgctcgg
      481 cctgggcgta tttttcgccc ttggacttcc gcatcacgaa gacgtccggc agcggcgcac
      541 tatgccgatc gatccgcaag gtggcggtga cgatgacctt gagggctggt cgtggcgaga
      601 gcgcgcgcaa ggcgctttcg attgcgaaca caacgtcggc attggcgccg ccatgatcga
      661 tcgacgccgg ggccatctgg atgatcttcc cttcgatcag ttcgacccgc ccctggacgt
      721 cggagaacac accagcttgg tccatcagtt caaactgttc gaagtcgaac aggaactcgc
      781 tgtcgccgcg ttcttcgaaa cccgcgttca tcgtcgtcat gatcgcatct ccgttcccga
      841 aacgtactgc tactctgccg cgtattgcaa gctgggctcg cggccgtcga acgtgacgaa
      901 ggtgcgttgt ggcgcggcgg tcgcggggaa gtcggcgcgg aaatgaccgc cccggctttc
      961 ctcgcgcgcc agggcggcgg cgacgatcag gcgggcggcg accagcggcg ggcaggggcc
     1021 ataggcaggc tccagggcgt cgatctggtc gagcaggcgg gtcaggccgg ccgcgtcgcg
     1081 gatcacgccg gcgtcgcggc tcatggcctt gcgcagggtc tggagcacat cggcgggcag
     1141 gtcgggcgtc ggctccagcg agacgggcag tccgggcgcg tgagcctcca tcgccgccgc
     1201 gcggccggcg cgggcgccga acacggccgc ttccagcagg ctgttggagg ccaggcggtt
     1261 ggcgccgtgc acgccggtgg cggcgcattc accggcggcg taaaggccgg gcagggaggt
     1321 ggcgccgtcc aggtcggtgg cgaccccgcc catgtgatag tgcacggcgg gggtgaccgg
     1381 gatcatctcg cggcgcggat cgatcccgcc cgagcggcag gcttcgaaca cggccgggaa
     1441 ctcgtgcgga aagtgggcgc cgaccgccgt cgtcgcgtcg aggaacgcgc cgcgaccggc
     1501 cgcgcgctcg gcgtggatgg cgcgggccac gatgtcgcgc ggggccagtt cggcggcagg
     1561 gtggtagtcg gccatgaagg cgcggccatc ggtgttgcgc aggatcgcgc cctcgccgcg
     1621 cagggcttcg gtggccaggg gtgcgggatc gcggccgatg tcgatggccg tcgggtggaa
     1681 ctgcacgaac tcgggatcgg cgatcaccgc gccggccaga gccgccaggc ccaggccctc
     1741 gccgcgcacc tcggcggggg tggtggtcac ggcatagagg ccgccgacgc cgccggtggc
     1801 caggatcacc gacgggctca ggatctcgac cagctggccg tcgatctctg caagaacacc
     1861 taagacgcgg cccgtcgcgt cctgaagaag ccggcgagca cgggcggtct cgcggatctc
     1921 gatggcgggc gccgcgcgga cggcggcgat gacggcggcc atgatcgctg cgccggcccc
     1981 gtcaccgccc acgcgggcga cgcgagcctt gctgtgtgcg gcttcgaggc tgagggtgaa
     2041 cgcgccgtcc tgggtgtcaa acggcgcgcc gagggcggcc aggaggcgaa cggcgtccgg
     2101 accttcgcgg gtcagcaggt ctacggcctc cggatcgcac aggccagcgc cggccgcgat
     2161 ggtgtcgtcg gcgtgcagcg ccggggcgtc gtcgtccgac agggccgcag ccatgccacc
     2221 ctgcgcccaa gcgctggagc agccggtcgc cagcggcgtc ggcgacagca ccagggctcg
     2281 cttcgctgcc cactttgtcg cggagagcgc ggcggtgagg ccggccagac cggcgcccag
     2341 gatcacgggg ccgtcgaaga tcacacggtc ggtcatttca cactccactc ccttccccct
     2401 ggatggggga agggccgggg atgggggtga acgcgaagcg ttcagccctc gacaacggcg
     2461 gggtggggca ggggtggctg aaactcagca tcacccccat cccaaccctt cccccatcaa
     2521 gggggaaggg ctcttagatc atttccacgt cgacgtgatg gcgggccttc acgaggtcgt
     2581 agcgggcggg aaccaggggc gggggcaggt cgatcatccg ttgcacggcc agtcgcgcgc
     2641 ggtcggcgat gacggggtcc accgtcacct cgaactgctc atgcaccagg gcgtcgtgga
     2701 tgttctgcag ggtgatgcgc ttcatgtgcg ggcacatgtt gcaggggccg atgaactggg
     2761 tggccgggct ttcggcctgg acgttggagg ccatcgagca ctcggtgatc agcaccacct
     2821 gcgccggctt gcgcgccgcc acatagtcgt t
//
LOCUS       DAOMEO010000330         2847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3849_length_2847_cov_2.609957, whole genome shotgun sequence.
ACCESSION   DAOMEO010000330 DAOMEO010000000
VERSION     DAOMEO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_2847_cov_2.609957"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLH31_08395"
     CDS             <1..528
                     /locus_tag="PLH31_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQR89268.1"
                     /translation="DAPYGRSFSYCTAGVTTLGAVVQSAVRTPLPAFADEALFGPLGI
                     EKPQWQFSPTGLAQGGGGLSLTSRDLLKLGQLYANGGRWNGQQILPEAWVKASVTPHA
                     EARTGTDYGYLWWLQTFAGHKAWLMNGSGGNKVIVIPDLDLVVVITTTNYNVRNSHGI
                     SDKLLVEHVLAAIGG"
     gene            561..>847
                     /locus_tag="PLH31_08400"
     CDS             561..>847
                     /locus_tag="PLH31_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQR89269.1"
                     /translation="MTQPTINIVTAVIRDDAGRMLVVRKRGTAIFMKPGGKREPGEDD
                     VTALARELDEELGCRLVEAKLLGRFEAPAANEPGHTVRAATYLAKVEGEVAV"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(902..1240)
                     /locus_tag="PLH31_08405"
     CDS             complement(902..1240)
                     /locus_tag="PLH31_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89270.1"
                     /translation="MWLTPRRALFDAFESRGAARLAAWLTLTGEATRMTSVRGAVEEV
                     INARISQVKGVSVEVMGDFILGCVTYALGVGLFGAPLGELMGKPPGRAREVALGILQA
                     RLDAAVGGAA"
     gene            complement(1200..1385)
                     /locus_tag="PLH31_08410"
     CDS             complement(1200..1385)
                     /locus_tag="PLH31_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89271.1"
                     /translation="MSIEHSDCRTRHRRSPEASRDNILAAAEAILLASGPQDLKLVEV
                     AKAAGVANATESAVRRV"
     gene            1457..2305
                     /locus_tag="PLH31_08415"
     CDS             1457..2305
                     /locus_tag="PLH31_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQR89272.1"
                     /translation="MATPRTTPEDVTVTPRDLHFDTQAAGKGAWLGGDVVGTAVFNAL
                     SLTFPDGERLFMDAVRHYRPKLSGKLLDDAKAFITQEAIHSREHVAMNGGLDRNRYPV
                     AAIEAQVRERTAMVRGRGPMAMLGATIALEHFTSMMADSFMREDDLFENTDPEMKRLW
                     QWHALEETEHKAVAFDVFQEVTKDWTPARRYKLRVRVMILISIMFTRNITRYATSLLV
                     ADGMKPGVARARVLWFLFGTPGLFRRCANEYWAWYRPSFHPWDHDNRERLTSLRDQFT
                     PAMAAE"
     gene            complement(2319..>2847)
                     /locus_tag="PLH31_08420"
     CDS             complement(2319..>2847)
                     /locus_tag="PLH31_08420"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89273.1"
                     /translation="GCFFIDAPVSGGQAGAETGQLTIMAGGDAEAYARIELTISAYAK
                     AVRRMGEVGTGQLTKMCNQIAIAGVVQGVAEALHFAKQAGLPTDDVLAAISKGSAQSW
                     QMENRWPTMARGEFEFGFAVDWMRKDLGIALAEARRNGAKLESTALIDQFYAEVQAMG
                     GHRWDTSSLVARLEK"
BASE COUNT          499 a          997 c          905 g          436 t
ORIGIN      
        1 gacgccccct atggccgcag cttcagctat tgcaccgccg gcgtcaccac actgggcgcc
       61 gtcgttcaga gcgccgtgag gacgccgcta cccgcctttg ccgacgaggc gctgtttggc
      121 ccgctgggca tcgagaagcc gcaatggcag ttctcgccca cgggcctggc ccagggcggc
      181 ggcggcctga gcctgaccag ccgagacctg ctgaaactgg gccagctcta cgccaatggc
      241 gggcgctgga acggccagca aatcctgccc gaagcctggg tgaaggcgtc cgtcaccccg
      301 cacgccgagg cgcgcaccgg aaccgactat ggctatctgt ggtggctgca gacctttgcc
      361 ggccacaagg cctggctgat gaacggcagc ggcggcaaca aggtgatcgt gatccccgac
      421 ctcgacctgg tggtggtgat caccaccacc aactacaacg tccgcaattc gcacgggatc
      481 tcggacaagc tgctggttga gcacgtgctg gcggcgatcg gcggctgagg cgaatccttc
      541 cgcctgctaa ggctgggtcc atgacccagc cgaccatcaa catcgtcacc gccgtcatcc
      601 gcgacgacgc cggccggatg ctggtggtcc gcaagcgcgg cacggcgatc ttcatgaagc
      661 ctggcggcaa gcgcgaaccg ggcgaggacg acgtcaccgc cctcgcccgc gaactcgatg
      721 aggaactggg ctgccggctg gtcgaggcca agctgttggg ccggttcgag gctccggccg
      781 ccaatgagcc gggacacacc gtgcgcgcgg cgacctacct cgccaaggtc gagggcgagg
      841 tcgctgtnnn nnnnnnngat atccgcttgg cgccgctgat gcgcgtagtt ctggcggccc
      901 tctaggcggc gccgcccaca gccgcgtcca gccgcgcctg caggatgccc agcgccacct
      961 cgcgggcgcg gccgggcggc ttgcccatca gctcgcccag cggcgcgccg aacaggccga
     1021 cccccagggc ataagtcacg cagcccagga tgaagtcgcc catcacctcg accgagacgc
     1081 ctttgacctg gctgatccgc gcgttgatca cttcctcaac tgcaccgcgc acgctcgtca
     1141 tgcgggtcgc ttcgccggtc agggtcagcc aggcggccag ccgcgccgcg ccgcgcgact
     1201 caaacgcgtc gaacagcgct ctccgtggcg ttagccacac cggcggcctt ggccacctcg
     1261 accagcttga ggtcctgggg acctgaggcc agcaggatcg cctcggcggc cgccaggatg
     1321 ttgtcgcggg acgcctcagg cgaacggcga tgccgggtac ggcagtcgga atgttctatt
     1381 gacatttttg tcggtaaggc caaactgcaa ccaagtccac agtgacaagc cctatcccga
     1441 ggcaggatcg aacaccatgg ccacgccgcg caccacacct gaagacgtca cagtcacccc
     1501 gcgcgacctg catttcgaca cccaggccgc cggcaagggc gcatggctgg gcggcgacgt
     1561 ggtgggcacg gccgtgttca acgccctgtc gctgaccttc cctgacggcg agcggctgtt
     1621 catggacgca gtgcgccact accgccccaa gctgtcgggc aagctgctgg acgacgccaa
     1681 ggccttcatc acccaggaag ccatccattc gcgcgagcat gtggcgatga acggcggcct
     1741 ggaccgtaac cgctatccgg tcgccgccat cgaggcccag gtccgcgagc gcaccgcgat
     1801 ggtgcgcggc aggggcccga tggccatgct gggcgcaacc atcgcactgg aacactttac
     1861 gtcgatgatg gccgactcgt tcatgcgcga ggacgacctg ttcgagaaca ccgatcccga
     1921 gatgaagcgc ctgtggcaat ggcacgccct ggaagagacc gagcacaagg ccgtggcctt
     1981 cgacgtgttc caggaagtca ccaaggactg gacgccggcg cgccgctaca agctgcgcgt
     2041 gcgggtgatg atcctgatca gtatcatgtt cacccgcaac atcacccgct acgccaccag
     2101 cctgctggtc gccgacggca tgaagccggg cgtcgctcgc gccagggtgc tgtggttcct
     2161 gttcggaacg cccggcctgt tccgtcgctg cgccaatgaa tactgggcct ggtatcgccc
     2221 cagcttccac ccgtgggacc acgacaaccg cgagcggctg accagcctgc gcgaccagtt
     2281 cacgccggcg atggccgcag agtagtcggc agcgccggct acttctccaa ccgcgccacg
     2341 aggctggacg tatcccagcg atggccgccc atggcctgca cctcggcata gaactggtcg
     2401 atcagggcgg tgctttccag cttggcgcca ttgcggcggg cctcggccag ggcgatgccc
     2461 aggtccttgc gcatccagtc aaccgcaaag ccgaactcga actcgccgcg cgccatggtg
     2521 ggccagcggt tctccatctg ccatgactgg gcgctgccct tggagatcgc ggccaggaca
     2581 tcatcagtag gcaggcccgc ctgcttggcg aaatgcaggg cttcggccac gccctggaca
     2641 acgccggcaa tcgcgatctg attgcacatc ttggtcagct ggccagtgcc cacctcgccc
     2701 atacgccgca cggccttggc gtaggcggaa atggtcagtt ctatccgggc ataggcctcg
     2761 gcgtcgccac cagccatgat ggtcagctga ccagtctcgg cgccagcctg accgcccgag
     2821 accggcgcat cgataaagaa acagccc
//
LOCUS       DAOMEO010000331         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3851_length_2845_cov_2.804301, whole genome shotgun sequence.
ACCESSION   DAOMEO010000331 DAOMEO010000000
VERSION     DAOMEO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3851_length_2845_cov_2.804301"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PLH31_08425"
     CDS             <1..970
                     /locus_tag="PLH31_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQR89274.1"
                     /translation="IIGAGMAGLACAQRLVQAGLSVRLLDKGRGAGGRMSTRRIQTSQ
                     GEACFDHGAAVFRVEDPAFRRCVEDWIGEGCVAPWPEAGPNAMVGLPTMNAPLKALAR
                     DLDVEWSAQVCGLSRGASGWMLDVVGRDPGSEGPFDVVVLAIPPEQATPLLSPHSFDL
                     AVYAAMAQSEAVWSAMVAFDSQLPLDVQRRGGCETLAVAIRENSKPGRSGPEAWTLQA
                     TGSWTQRRLDTSAGEVATLLLEALACRLDGPLPQVLALQAHRWRYARPARLTSGPIWR
                     AGVGLGLCGDWVAGDGVEAAWCSGVRLADRISGRVRSGFEQDCAAP"
     gene            complement(1000..1842)
                     /locus_tag="PLH31_08430"
     CDS             complement(1000..1842)
                     /locus_tag="PLH31_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQR89275.1"
                     /translation="MAAAAPALATPRPTLVLTEIPKPAGPATVLFNGQDLKDWDAWLG
                     YADPALTYKRPPVAPIGATARSAEMFKVVVEDGRPALYINGRTWGSLVRRGDFQNYHL
                     RLQYKWGKGRWAPREAEAPNNGLLYHSFGAPGVVWGTWSQSAEFEIMLGSTGMIVPVG
                     EAISAVTTVIDDPTLINPKLRFAPDGRVKTVKGGTADWNVEAYSDAEKPAGEWNTLDL
                     YVLGDRAVHVVNGVPVMEVSDLRADDQPLTHGAIQLQSEGAETFFRDMVLTPITALPK
                     VVAK"
     assembly_gap    1857..1866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2002..>2191
                     /locus_tag="PLH31_08435"
     CDS             2002..>2191
                     /locus_tag="PLH31_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQR89276.1"
                     /translation="MTTPSVVLAAAIGLLAAAPAQNRPEDTEVWTPVPAKVEPAATAG
                     QAPADAIVLFDGDDLDQWV"
     assembly_gap    2192..2201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2202..2582
                     /locus_tag="PLH31_08440"
     CDS             <2202..2582
                     /locus_tag="PLH31_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQR89277.1"
                     /translation="EGYEVQILDSYGNATYANGQAASVYKQHPPLANASRRPGEWQTY
                     DIVWRAPAFGADGALVSPAKVTVLHNGILVQDNAVLAGETVYIGKPSYRLHGATPIKL
                     QAHGDPSVPISFRNIWLRELAPRP"
     gene            complement(2579..>2845)
                     /locus_tag="PLH31_08445"
     CDS             complement(2579..>2845)
                     /locus_tag="PLH31_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR89278.1"
                     /translation="EMAMGALEAIRQRGLRCPDDISLVGFDDIRFAQYLNPKLTTVSQ
                     PMEQIGHEVVRLLLDILADRASALQNVTLPHRLVVRESTAPPPA"
BASE COUNT          423 a          942 c          994 g          466 t
ORIGIN      
        1 gatcattggc gcgggcatgg ctggcctggc ctgcgcccag cgtctggtgc aagcgggcct
       61 ttcagtgcgg ctgttggaca aggggcgcgg ggctggcggc aggatgtcga cgcgtcgaat
      121 ccagacgtcg cagggcgaag cctgctttga ccatggcgcg gcggtcttca gggtcgaaga
      181 tcccgccttc cggcgatgcg ttgaggactg gatcggcgag gggtgcgtgg ccccctggcc
      241 ggaggcgggc cccaacgcca tggtgggcct gccgaccatg aacgctccgc tcaaggccct
      301 ggcccgcgac ctggatgttg aatggtccgc ccaggtctgc ggtctgtcac gcggggcgtc
      361 gggctggatg ctcgacgtgg tcggccgcga tcccggatcc gaagggccct tcgacgttgt
      421 tgtcctggcc atcccgcctg aacaggccac gccgctcctg tcgccccatt cgtttgatct
      481 cgccgtctat gccgcgatgg cgcaatccga agcggtctgg tcggccatgg tggcgttcga
      541 cagccaactg ccgctcgacg tccagcgacg gggcggctgc gagaccctgg ccgtcgcgat
      601 ccgggagaac agcaagcccg gtcgttcggg gcccgaggcg tggaccttgc aagccacagg
      661 cagctggacc cagcgacgcc tggacaccag cgccggcgag gtggcgaccc tgctgctgga
      721 ggcgctcgcc tgtcgccttg acgggccctt gccgcaggtt ctggccctgc aggcgcatcg
      781 ctggcgctat gcacgccccg cccggctcac atcggggccc atctggagag ccggcgtggg
      841 cctgggtctg tgcggcgact gggtcgcggg cgacggcgtc gaggcggcct ggtgctcagg
      901 cgttcgtctg gccgaccgca tcagcgggcg ggtgcgctcg ggctttgaac aggactgcgc
      961 tgcaccctag cgggcggaga gtcgcctttc gggcgccgcc tatttcgcca ctaccttggg
     1021 cagggccgtg atcggcgtca gcaccatgtc gcggaagaag gtttcggcgc cttccgactg
     1081 caactggatc gcgccgtggg tcaggggctg gtcgtcagcc ctgagatcgc tgacctccat
     1141 caccggcacg ccattgacca catgcaccgc gcggtcgccc agcacgtaga ggtccagggt
     1201 gttccactcg ccggccggct tttcggcgtc gctataggcc tcgacattcc agtcggctgt
     1261 accgcccttg accgtcttga cgcgaccgtc cggcgcgaac cgcagcttgg gattgatcag
     1321 ggttggatcg tcgatcaccg tggtgacggc gctgatcgcc tcgcccacgg gcacgatcat
     1381 gccggtcgag cccagcatga tctcgaactc cgcggactgg ctccaggtgc cccagaccac
     1441 gcccggcgcg ccgaacgaat ggtagagcag gccgttgttg ggcgcttcgg cctcgcgcgg
     1501 cgcccagcgg cccttgcccc acttgtactg caggcgcagg tgatagttct ggaagtcgcc
     1561 cctgcggacc aggctgcccc aggtcctgcc gttgatatag agggcggggc ggccatcctc
     1621 gaccaccacc ttgaacatct cggcgctccg ggctgtcgcg ccgatcgggg cgacgggcgg
     1681 gcgcttgtag gtcagggccg ggtcggcata gcccagccac gcgtcccaat ccttcaggtc
     1741 ctggccgttg aacagcaccg tcgcggggcc ggcaggcttg gggatctcgg tcagaaccag
     1801 ggtcggacgc ggggtggcca aggccggcgc ggcggcggcc agggcggcga caaaggnnnn
     1861 nnnnnngacg cgaccttatc tgacagcgtt gtcaaggcaa gcccatggat cgctgttgcg
     1921 gccaccttgg cgaagcggcg tctaggccgc cccggcagga ggcgataaga cagagcggtt
     1981 ctggagggag cgcgacataa catgacgacg ccaagcgtgg ttctcgccgc cgcgatcggc
     2041 ctgctggccg cggcgcccgc ccagaacagg cccgaggaca ccgaagtctg gacgccagtt
     2101 ccagccaagg tcgagcccgc ggcgaccgcc ggccaggcgc cggccgacgc catcgtgctg
     2161 ttcgacggcg atgatctgga ccagtgggtc tnnnnnnnnn ngaaggctat gaggttcaga
     2221 tcctcgactc gtacggcaac gccacctacg ccaacgggca ggccgccagc gtctacaagc
     2281 agcatccgcc actcgccaat gccagccgta ggccgggcga gtggcagacc tatgacatcg
     2341 tctggcgagc gccggctttt ggcgcggacg gcgcgcttgt ttcgccggcc aaggtgacgg
     2401 tgctgcacaa tggcatactg gtgcaggaca acgcggtgtt ggccggcgag accgtctata
     2461 ttggcaaacc ctcgtatcgc ctccacggcg cgacgccgat caagctgcag gcccatggcg
     2521 accccagcgt gccgatcagc ttccgcaata tctggctgcg cgagcttgcg ccccgtccct
     2581 aggccggcgg cggggcggtg ctttcccgca ccaccaggcg gtggggcagg gtgacattct
     2641 gcagcgcgct ggcccggtcg gccagaatgt cgagcaatag ccggaccacc tcatggccga
     2701 tctgctccat cggctggctg accgtggtca gtttggggtt caggtattga gcgaagcgga
     2761 tgtcgtcgaa gccgaccagc gatatgtcgt ccgggcagcg caggccgcgc tggcggatgg
     2821 cctccagcgc gcccatggcc atctc
//
LOCUS       DAOMEO010000332         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3852_length_2845_cov_2.612186, whole genome shotgun sequence.
ACCESSION   DAOMEO010000332 DAOMEO010000000
VERSION     DAOMEO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3852_length_2845_cov_2.612186"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PLH31_08450"
     CDS             <1..174
                     /locus_tag="PLH31_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphatase"
                     /protein_id="HQR89279.1"
                     /translation="IAAVAQGPDALASESADLLYHWLVLMAASDVSLDAVADKLAARE
                     GISGIAEKASRPQ"
     gene            complement(355..909)
                     /locus_tag="PLH31_08455"
     CDS             complement(355..909)
                     /locus_tag="PLH31_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQR89280.1"
                     /translation="MKRPTRPEELRLWSMVADTVRARPGDKPLKLRAKVKVKPVKAPK
                     AEKTQPTYLASIAPLRIDPAEIKPAPVRTPKSAPGHIEPNRRKRLIKEHDPIGARLDL
                     HGYDQDQARPALEAFIRRAHADGHRAVLVITGKGKIGHGVLKKRTPEWLAAPALREMI
                     AGVSQADQRHGGEGALYVALKRRA"
     gene            complement(912..2054)
                     /locus_tag="PLH31_08460"
     CDS             complement(912..2054)
                     /locus_tag="PLH31_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HQR89281.1"
                     /translation="MLRVGLIAVLASLTLAACTTTAPPVATGPVAPLPAPSLPATPGG
                     PTLAFSTLPGWQEEDHLAALDAFRAGCGAAREQEMARVCRLARTDGPRDDWGARLFLE
                     ANFRPQAVGDQGLLTAYFAPKYDARLSRSDEFSAPVRGVPSDLVVLDLGAFEPSFAGK
                     KVTGRVKGGKFEPYPDRKTIEASPVDKPLAWMRPEELFFLQIQGSGILVMPDGRRMRA
                     AFAGGNGKPFVGIANAMRDRGLLPDNNTSGDAIRTWLADNRGPRADEIMQLNPRYIFF
                     KLSEDDGKEPVGSAGIPLPAGRAIAVDPSRHAMGGLFWLDAAAPKLAGAFPVYRRLAV
                     ALDVGGAIKGEVRADLYTGTGATAGAEAGRVRHDLRMYRLVPIPKS"
     gene            complement(2054..2653)
                     /gene="timA"
                     /locus_tag="PLH31_08465"
     CDS             complement(2054..2653)
                     /gene="timA"
                     /locus_tag="PLH31_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-related membrane protein TimA"
                     /protein_id="HQR89282.1"
                     /translation="MLIQILVLAVIAAVVLYQLYATLGRRVGRQPEDGPAIPKTVPAP
                     ATPLEPVTAASLEGVAALRARQPDFDTGHFLSGARAAYEMIVTAFAAGDRETLKPLLA
                     DDVMANFETAMVARETEGRSEKVELMAPPRVDLDNVTVVGDAARATVRILAEVRARSS
                     GPEGDAVDDRRTAELWTFERDLKSANPNWTLVHVAAAEA"
BASE COUNT          423 a          955 c         1006 g          461 t
ORIGIN      
        1 atcgccgccg tcgcccaggg gccggacgcc ctggcgagcg agagcgccga cctgctgtat
       61 cactggctgg tgctgatggc cgcctcggac gtgtcgctcg acgccgtggc cgacaagctg
      121 gcggcgcgtg aagggatatc ggggatcgcc gagaaggctt cgcggcctca gtaggccgcc
      181 attctacctt ttcttccgtc attcccgccc ttgtggcggg aacccctcgt accgccgcag
      241 gtgcagtagg ggcgaagtgc ctgcgcactt cgctggctct tcacgtgtca gctgaaacag
      301 gggttcccgc cacaagggcg ggaatgacgg agcttgatgt gagccttcgc gcgcttaagc
      361 ccgccgcttg agcgccacgt agagcgcccc ctcgcccccg tgccgctggt cggcctgcga
      421 caccccagcg atcatctcgc gcaaagcggg cgccgccagc cactccggcg tgcgcttctt
      481 cagcacgcca tggccgatct tgcccttgcc ggtgatcacc aacaccgccc ggtgaccatc
      541 ggcatgagcg cggcgaatga aggcctcaag ggccggcctg gcctggtcct ggtcatagcc
      601 gtgcaggtcc agccgcgcgc cgatcgggtc atgttccttg atcaggcgct tgcgacggtt
      661 gggttcgata tggccggggg cggactttgg cgtcctgacc ggggccggct tgatctcggc
      721 cgggtcgatg cgcagcggcg cgatcgaggc cagataggtc ggctgggtct tctcggcctt
      781 gggcgccttc accggcttga ccttgacctt ggcccgcagc ttcagcggct tgtcgcccgg
      841 ccgcgcgcgc accgtgtcgg cgaccatgct ccagaggcgc agctcttccg gtcgcgtcgg
      901 ccgtttcatg actacgattt cgggatcgga accaggcggt acatccgcag gtcatggcgc
      961 acgcgcccgg cctcggcgcc ggcggtggcg cccgtgcccg tatagagatc ggcccggacc
     1021 tcgcccttga tcgcgccgcc gacgtccagc gccacggcca ggcggcgata gacgggaaac
     1081 gcgccagcca gcttgggcgc ggcggcgtcc agccagaaaa gaccgcccat ggcgtggcgg
     1141 ctgggatcca cggcgatggc ccggcccgcc ggcagcggaa taccggctga gcccaccggc
     1201 tccttgccgt cgtcttccga cagcttgaag aagatgtagc gcgggttcag ctgcatgatc
     1261 tcgtcggccc tggggccgcg attgtcggcc agccaggtgc ggatcgcatc gccggaggtg
     1321 ttgttgtcgg gcagcaggcc ccggtcgcgc atggcgttgg cgatgccgac aaagggcttg
     1381 ccgttgccgc cggcgaaggc ggccctcatg cggcgtccat cgggcatcac cagaataccc
     1441 gagccctgga tctgcaggaa gaacagctct tctggccgca tccaggccag gggcttgtcg
     1501 acgggcgagg cttcgatggt cttgcggtcg ggatagggtt cgaacttgcc gcccttgacc
     1561 cggccggtga ccttcttgcc cgcgaagctg ggctcaaagg cgccgagatc cagaacgacc
     1621 agatcgctcg gcacgccgcg caccggggcg ctgaactcgt ccgagcgcga cagacgggcg
     1681 tcgtacttcg gggcgaaata ggcggtcagc aggccctggt cacccacggc ctggggccgg
     1741 aaattggctt ccaggaacag tcgcgcgccc cagtcgtcgc gcggaccgtc ggtacgggcc
     1801 aggcggcaga cgcgcgccat ctcctgttcg cgcgccgcgc cgcagccggc ccggaaggcg
     1861 tccagggcgg ccaggtgatc ttcttcctgc cagcccggca gggtgctgaa ggccagtgtc
     1921 ggaccgccag gcgtcgctgg aaggctgggc gcgggaagcg gcgcgacggg ccctgtcgcg
     1981 actggcggcg ccgtggttgt acaggccgcc agggtcagcg aggccagaac ggcgatcagg
     2041 ccaacgcgca gcatcaggct tcggcggccg cgacgtggac cagggtccag ttggggttgg
     2101 ccgacttcaa gtcgcgctcg aaggtccaca gttcggcggt gcggcggtcg tcgacggcgt
     2161 cgccctcagg cccgctggag cgggcgcgaa cctcggccag aatgcggaca gtcgcccggg
     2221 cggcgtcgcc gaccacggtc acattgtcga gatcgacacg cggcggggcc atcaactcga
     2281 ccttctcgga ccggccctcg gtttcgcggg cgaccatggc cgtctcgaaa ttggccatga
     2341 cgtcatcggc tagcaggggc ttgagcgtct cgcgatcgcc cgccgcgaag gccgtgacga
     2401 tcatctcgta ggccgcgcgg gcgccgctca ggaagtggcc ggtatcgaag tccggctggc
     2461 gcgcgcgcag ggcggcgacg ccttccagag acgcggcggt gacgggttcg agcggcgtgg
     2521 cgggggccgg aacggtcttc gggatcgccg gtccgtcttc gggctgacgg cccacgcgac
     2581 ggcccagcgt cgcatagagc tggtagagga cgacggcggc gatgaccgcc aaaacgagaa
     2641 tctgaataag cacttggata tccgcggctg cgtcgacaag ctggtcgcca tgtctatata
     2701 ggcgaggcga gctctctcgt caggcgcctg gcgttgcgcc agacccgccc gcgtgttagc
     2761 agcgccgctc tctcctagcg gtctttttag acgccgagta cggatttatt cctaatgacc
     2821 gatacgaccg ccgcccctga ggcgc
//
LOCUS       DAOMEO010000333         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3858_length_2839_cov_2.304957, whole genome shotgun sequence.
ACCESSION   DAOMEO010000333 DAOMEO010000000
VERSION     DAOMEO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3858_length_2839_cov_2.304957"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..805
                     /locus_tag="PLH31_08470"
     CDS             299..805
                     /locus_tag="PLH31_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89283.1"
                     /translation="MIHHLSVSAADPQGVGEFFADLMNGVVVDFPPNPGSCMVFKADG
                     LGTAIEIYPAGSVMERNGEPGAIFVRQPDGADRRSPTHFALSVEMSPDEVLARAAAKG
                     WDAFVCDRGGHFHVVEVWIENNWLVELLPPAFAAEYLGFANEVSTMSDPNAALDSHQP
                     QAKTLEPA"
     gene            complement(849..>1095)
                     /locus_tag="PLH31_08475"
     CDS             complement(849..>1095)
                     /locus_tag="PLH31_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR89284.1"
                     /translation="DAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEV
                     GQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDS"
     assembly_gap    1096..1105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1106..2808)
                     /gene="rpoD"
                     /locus_tag="PLH31_08480"
     CDS             complement(<1106..2808)
                     /gene="rpoD"
                     /locus_tag="PLH31_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HQR89285.1"
                     /translation="MSNNSSADTEAPESTGGDGPLLDLTDAGVKKFIKQAKARGFVTM
                     EELNKVLPSEEVSPDAIEDTLAMLSEMGVNVVEAEEDAETNEGGEVVAREDNQQVITS
                     DKPSAYDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRDTMIRGLCESALTFEA
                     IMVWREELGTGRILLREVIDLEGTYAAINGVAAQPAADDTEAEPAEPVDEDAAPKPEA
                     EGDDEDDFDDGAGPTVSAMEGELREGVMAILDAIAGEFETFRKLQDKLVGSRLRGEDL
                     SDNDRKAYEALSQTIILHLKTLKLNNNRIEALVEQLYAINKRLIGLEGRLLRLADSYG
                     ISRSEFLKAYFGSELNPSWSDQVKAMGVRWTKFVENDATSVTDIRSEIAALATETGVP
                     IDDYRRIVQTVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
                     MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQM
                     LHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNA
                     IL"
BASE COUNT          429 a          879 c          922 g          599 t
ORIGIN      
        1 agttggccat cgacataggc gcccagccgg ggaagcaggt cgagcagggc ggtctgaaac
       61 gtatccaagg tcgctgccgt cgcccgatcc cgccggggcg atggtgcccg tcgaggtgtc
      121 aaaaattaaa tcgaggtcat gaagaccttg taagttgagg cacagcggcc cgccgaataa
      181 atcccccgac ctcggcgcga taacgtcgat atttcatcag cacggcgagt ctaacacaag
      241 gcgcgcggca gaggtcgcgc gcgtacagtt caactacgcc tcattcggag gtattctcat
      301 gatccatcac ctgtccgtta gtgcggcgga ccctcaaggc gtcggcgaat tcttcgctga
      361 tctcatgaac ggcgtcgtcg tcgattttcc gcccaatccg ggtagctgca tggtgttcaa
      421 ggccgacggt ctgggcacgg ccatcgagat ctatccggcc ggcagcgtca tggagcgcaa
      481 tggcgagccc ggcgcgatct ttgtgcgcca gccggacggc gccgaccgcc gctcgcccac
      541 ccacttcgcc ctgagtgtgg agatgagccc ggacgaggtc ctcgcccgcg ccgccgccaa
      601 aggctgggac gcctttgtct gcgatcgggg cggccacttc catgtggtcg aggtgtggat
      661 cgagaacaac tggctggtcg agttgctgcc gccagcgttc gcggccgagt atctgggctt
      721 cgccaatgag gtctcgacca tgtccgaccc caatgcggcg ctggactcgc accagccgca
      781 ggccaagacg ctggaaccgg cctgaggtca gaaaaaaggg cggctcgtga gagccgccct
      841 ttgtgatcct agctgtccag gaagctccgc agcttccggc tccggctcgg gtgcttcagt
      901 ttgcgcagcg ccttggcctc gatctgacgg atgcgttcgc gggtgaccga gaactgctgg
      961 ccgacttctt ccagggtgtg gtcggtgttc atgccgatgc cgaaacgcat ccgcagcacg
     1021 cgctcttcac gcggcgtcag cgaggccagc acgcgggtgg tggtttcccg caggttgctc
     1081 tggatggccg cgtcgnnnnn nnnnnaggat ggcgttcttg tcttcgatga agtcgcccag
     1141 gtggctgtct tcctcgtcgc cgatcggcgt ttcgaggctg atgggctcct tggcgatctt
     1201 caggaccttg cggacctttt ccagcggcat ggccagcttt tcggccagct cttcgggggt
     1261 cggttcgcgg ccgatctcgt gcagcatctg acggctggtg cggacgatct tgttgatcgt
     1321 ctcgatcatg tggaccggga tgcggatggt ccgcgcctgg tcggcgatac tgcgggtgat
     1381 ggcctgacgg atccaccagg tggcgtaggt cgagaacttg tagccgcggc gatattcgaa
     1441 cttatcgacg gccttcatca ggccgatatt gccttcctgg atcaggtcca ggaactgcag
     1501 gccgcggttg gtgtacttct tggcgatgga gatcacgagg cggaggttgg cctcgaccat
     1561 ttccttcttg gcctgacggg cctcgcgctc gcccttctgg acggtctgga caatgcggcg
     1621 atagtcgtcg atcggcacgc cggtctcggt ggccagggcg gcgatctcgc tgcggatgtc
     1681 ggtgaccgag gtggcgtcgt tctcgacgaa cttggtccag cgcacgccca tggccttgac
     1741 ctggtcgctc cagctcgggt tcagctcgga gccgaaatag gccttcagga actcgctgcg
     1801 gctgatgcca tagctgtcgg ccaggcgcag cagacggcct tccagaccga tcaggcgctt
     1861 gttgatcgca tacagctgct cgaccagggc ctcgatgcgg ttgttgttca gcttcagggt
     1921 cttcaggtgc agaatgatgg tctgcgacag ggcctcatag gccttgcggt cgttgtcgct
     1981 gaggtcttcg ccgcgcaggc ggctgcccac cagcttgtcc tgcagcttgc ggaaggtttc
     2041 gaactcgccg gcgatggcgt cgagaatggc catcacgcct tcgcgcagtt cgccttccat
     2101 ggcgctgacg gtcgggccgg cgccgtcgtc gaaatcgtcc tcgtcgtcgc cttcggcttc
     2161 cggcttgggc gcagcgtcct cgtccaccgg ctcggcgggc tcggcttcgg tgtcgtcagc
     2221 ggccggctgg gcggcgacgc cgttgatcgc ggcgtaggtg ccttccaggt cgatcacttc
     2281 gcgcagcagg atgcggcccg tgcccagttc ctcgcgccag accatgatgg cttcaaaggt
     2341 cagggcgctt tcgcacagac cccggatcat ggtgtcgcga ccggcttcga tccgcttggc
     2401 gatggcgatc tcgccttcgc ggctcagcag ttcgacgctg cccatctcgc gcaggtacat
     2461 gcgcacgggg tcatcggtgc gatcataggc cgacggcttg tcggaggtga tgacctgctg
     2521 gttgtcttcg cgcgcgacga cttcgccgcc ctcgttggtt tcggcgtctt cttcggcttc
     2581 gacaacgttg acgcccatct cgctgagcat ggccagcgtg tcttcgatgg cgtccgggct
     2641 gacttcttcc gaaggcagca ccttgttcag ctcttccatc gtcacgaagc cgcgggcttt
     2701 cgcctgcttg atgaacttct tgacgccggc gtcggtcagg tcgagcagcg gaccatcacc
     2761 accggtggac tcaggggctt ccgtatcggc cgaggaatta ttgctcatca aggtctccga
     2821 agtcgacgca gcctccccc
//
LOCUS       DAOMEO010000334         2836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3862_length_2836_cov_2.907228, whole genome shotgun sequence.
ACCESSION   DAOMEO010000334 DAOMEO010000000
VERSION     DAOMEO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_2836_cov_2.907228"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLH31_08485"
     CDS             complement(<1..1349)
                     /locus_tag="PLH31_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQR89286.1"
                     /translation="MPDLMRRDLLLASAAMIALPGVALPGVSLPGVSGATPLEAAADL
                     RLWYRQPAKAWVEALPVGSGRLGAMVFGGVASERLQLNENTLWGGGPYNPVNPEAAAA
                     LPEIRRLIDAGDYAGATRLADAKFMATPLRQMSYQTLGDLRLTFPGLPETAQGYVRDL
                     DLDAATARVRFSHAGVAHVREVIASAPDQVIAVRLGADRPGAVSVDLAFDSPLKPGIS
                     VENGDTLVLSGRNAGQHGIASALTVDCRVKVVARGGKVIASATGLSVRDADEVLLLAA
                     AATSYRRFDDVSGDPAADDRARLARASRKPWSALLAAHRADHRRLFRKVSVDFGTTGA
                     ALRPTDERIRLSPTTHDPALAALYFQYGRYLLIACSRPGGQPANLQGLWNDQMNPPWG
                     SKFTININTEMNYWPAEPTGLADCVKPLIEMVRDLSITGARTARAMYGARGWVAHHNT
                     "
     gene            complement(1442..2053)
                     /locus_tag="PLH31_08490"
     CDS             complement(1442..2053)
                     /locus_tag="PLH31_08490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR89287.1"
                     /translation="MNNAWIARRSLLGGAGLLLASPLGAQAQTAKPRVAIQTSHGVIV
                     VELEDKKAPITSANFLRYVDKKKFDGGSFYRASRARGAPEKGSIQGGPSYRTRRFAPI
                     AHESTRLTGIRHKAGAISMARNEPGSATADFFICASPMAYLDARPGAPGDNQGFAAFG
                     TVVKGMDVVRKILASKTDGVPQNPSMKGQMINPPVAMVSVRRV"
     gene            2207..>2836
                     /locus_tag="PLH31_08495"
     CDS             2207..>2836
                     /locus_tag="PLH31_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQR89288.1"
                     /translation="MSTQTTHAFARTDRTAIGLWWWTTDRWLLGATAILVTLGVLLSF
                     ASSPAAAQRIGIDDQFHFALRMCVFASASTVILLGTSMLSPKGVRRAAFFIYVAAIAV
                     MIALPFIGHNAKGATRWVQFAGFTLQPSEFMKPALIVLVSWMFAEGQKGEGVPGVSIA
                     FLLYGIAVGLLLIQPDVGQTVLITVAFGAAFWMAGVPISWIMGLGAVAMA"
BASE COUNT          406 a          971 c          992 g          467 t
ORIGIN      
        1 gtattgtggt gcgccaccca gccgcgcgcg ccgtacatcg ccctggccgt ccgcgcgccg
       61 gtgatcgaca ggtcgcgcac catctcgatc aggggtttga cgcagtcggc gaggcccgtg
      121 ggctcggccg gccagtagtt catctcggta ttgatgttga tcgtgaactt gctgccccag
      181 ggcgggttca tctggtcgtt ccacaggccc tgcagattgg ccggctgccc gccggggcgc
      241 gagcaggcga tcagcaggta gcggccatac tggaaataca gcgccgccag ggccggatcg
      301 tgggtggtcg gcgagaggcg gatgcgttcg tcagtcggcc gcagggccgc gcccgtggtt
      361 ccaaagtcga ccgagacctt gcggaaaagg cggcgatgat cggcccggtg cgcggccagc
      421 agcgcgctcc acggcttgcg ggaggccctg gccaggcggg cgcggtcgtc ggcggcggga
      481 tcgcccgaca cgtcgtcaaa gcggcgatag ctggtggcgg cggctgccag cagcagcacc
      541 tcgtcagcgt cgcgcaccga caggcccgtg gcgctggcga tcaccttgcc gccccgggcc
      601 acgaccttga cccggcagtc caccgtcaag gcgctggcga tcccgtgctg gccggcattg
      661 cggccgctga gcaccagggt gtcgccgttc tcgacgctga tgccgggctt cagcgggctg
      721 tcgaaggcca ggtccacgct aaccgcgccc ggcctgtcgg cgcccagccg cacggcgatc
      781 acctgatcgg gcgcgctggc gatcacctcg cgaacatggg cgacgccggc gtgcgagaag
      841 cggacgcggg cggtggcggc gtccagatcc agatcgcgga catagccctg ggccgtctcg
      901 ggcaggccgg ggaaggtcag tcgcaggtcg ccaagggtct gatacgacat ctgccgcagc
      961 ggcgtggcca tgaacttcgc gtccgccagc cgggtggccc ctgcatagtc gccggcgtcg
     1021 atcaggcggc ggatttcggg cagggcggcg gcggcttcgg gattgaccgg gttgtaggga
     1081 ccgccgcccc acagggtgtt ctcgttcagc tgcaggcgtt cgctggcgac gccgccaaac
     1141 accatggcgc ccaggcgtcc ggagccgacc ggcaaggcct cgacccacgc cttggccggc
     1201 tgacgatacc agaggcgcag gtcagcggcg gcctccagcg gtgtcgcccc agagacgcca
     1261 ggcagagaga cgccaggcag agcgacgcca ggcagggcga tcatggcggc gctcgccagc
     1321 aacaggtcgc ggcgcatcaa atccggcatg gcgttcgatc cctggctccg cctcggaagg
     1381 acggattagg cgtccatcct tagctttggg accggtatca ttgcaaggat cgcggctgcc
     1441 cctagacccg ccgcacgctc accatcgcta ccggcggatt gatcatctgg cccttcatgc
     1501 tggggttctg cggcacgccg tccgtcttcg acgccaggat cttgcgcacc acgtccatgc
     1561 ccttgaccac cgtaccgaag gccgcgaagc cctggttgtc gccgggcgcg ccgggcctgg
     1621 cgtcgagata ggccatcggg ctggcgcaga tgaagaagtc ggccgtggcg ctgcccggct
     1681 cgttgcgggc catcgagatg gcgccggcct tgtggcggat gccggtcagc cgcgtgctct
     1741 cgtgcgcgat cggggcaaag cggcgggtgc gataggacgg cccgccctgg atagagccct
     1801 tttccggcgc gcccctggcc cgcgaggcgc gatagaagct gccgccgtcg aacttcttct
     1861 tgtcgacata gcgcaggaag ttggcggacg tgatcggggc cttcttgtct tccagttcca
     1921 ccacgatcac gccgtgcgag gtctggatcg ccacccgggg cttggcggtt tgggcctgtg
     1981 cgcccagcgg gctggccagc agaagtccgg ccccgcccag caggctgcgg cgtgcgatcc
     2041 aggcgttgtt catgggcatc ctcggcgagc aaaacagcgg cttcacccat aaggcgccgc
     2101 cgccgcgcca gccacacccg aaagcaccgc aacctttcgc cgcatatccc cgtccgggaa
     2161 gcgtggcagc aatgttccat taaccctgcg cgccgactct ccgtccatgt cgacccagac
     2221 cacccacgcc tttgcccgca ccgaccgcac cgcgatcggc ctgtggtggt ggacgaccga
     2281 tcgctggctg ctcggcgcca cggcgatttt ggtcaccctg ggcgtgctgc tgtcgttcgc
     2341 ctccagcccg gcggcggccc agcggatcgg catcgacgac cagttccatt tcgccctgcg
     2401 gatgtgcgtc ttcgccagcg cctcgacggt gatcctgctg ggcacctcga tgctatcgcc
     2461 caagggcgtg cggcgggcgg ccttcttcat ctatgtggcg gccatcgcgg tgatgatcgc
     2521 cctgccgttc atcggccaca acgccaaggg cgccacccgc tgggtgcagt tcgccggctt
     2581 caccctgcag ccgtccgagt tcatgaagcc ggccctgatc gtgctggtct cgtggatgtt
     2641 cgccgagggc cagaagggcg agggggtgcc aggcgtctcc atcgccttcc tgctgtatgg
     2701 cattgccgtg ggcctgctgc tgatccagcc cgacgtgggc cagaccgtgc tgatcaccgt
     2761 ggccttcggc gcggcgttct ggatggcggg cgtgccgatc agctggatca tgggcctggg
     2821 cgccgtggcc atggcg
//
LOCUS       DAOMEO010000335         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3865_length_2833_cov_4.033117, whole genome shotgun sequence.
ACCESSION   DAOMEO010000335 DAOMEO010000000
VERSION     DAOMEO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3865_length_2833_cov_4.033117"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..658
                     /locus_tag="PLH31_08500"
     CDS             380..658
                     /locus_tag="PLH31_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HQR89289.1"
                     /translation="MGHTVREKSKLLGRVRRIRGQVEAIERALEAETECAAVLQLIAS
                     VRGAMNGLMAEVMEDHIRMHVVDPASDPDPERARGAEELIDVVRTYLK"
     gene            673..2706
                     /locus_tag="PLH31_08505"
     CDS             673..2706
                     /locus_tag="PLH31_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQR89290.1"
                     /translation="MADQLNRDRTTLADQAEARTTAPGALNEKKHPHHGHDPDDHDDE
                     DSHDHEHPFEWQEALRIGLVAVAAALVWFRVWEPIPTVSVIGVVGLLIGGWPILKEAF
                     ENILQRRMTMELSMTIAIVAAAAIGQFFTALVITLFVLVAEVLEGLTVGRGRKAIRDL
                     LEFLPSEVSVRRTGSIRTISAAELNVGDAILVAPGGRIPVDGAVLSGNSFVDESRITG
                     ESMPVEKIEGAAVFAGSINQSGGLEVRAERIGRDTSYGKIIEAVERAERSRAPVQRLA
                     DRMAGYLVYFALGSAVLTYLITQNINSTISVIIVAGACGIAAGTPLAILGGIGRSARL
                     GAIIKGGVHLETLGKVDTVVLDKTGTLTFGRPEVQALVAAPGVSAEDLLDAAAAAELR
                     SEHPLGKAIITHAKAKGRPVAEPEHFAYTPGRGIAARVTGSLILVGNQAWMRENGVAI
                     LASLNFGVEAASEVYVARDGALLGAITVADTVRPEAKRAIESLTALGIRTLLLTGDAL
                     PVAQAVGRSLGIKEIEASLLPEDKLARIQALILEKRTVAMVGDGVNDAPALAEASVGI
                     AMGSGTDVAQESADIVLLGNDLVCLVETLKVARRTRRIIWQNFAGTLAIDAIGIGLAA
                     VGILNPLMAAFIHVASEMAFILNSARLLPRKVGADDGGAPSTPFGVQPAAVYP"
BASE COUNT          506 a          908 c          917 g          502 t
ORIGIN      
        1 aggctgatgg ccttgggcgc cgccaccgtg gcgctggccg ccggcgtcga cttgaccaga
       61 tgggcgtgag ccgaagccgt gccggccagc agcagggcgg cgctcgcggc gatcccggcg
      121 gcgatgaaat gatggtggcg catgcgattt ccttggctgg acggtgtccc tatcgaagat
      181 acgcgcggcc cggccccagc ccctcaagat gacgcagcga gctgcgcgac ctcaccaggg
      241 cagcttggcg ctgaggcctt cccccattcg atcaccctaa gctggggcat gcccttaggt
      301 tgaccggccg ccactattgg ctaaaagctt ggacattata gcctaccccc ctatactatt
      361 tccatagcgc aggtggtcga tgggacatac ggttcgcgaa aaatccaagc tgctcggtcg
      421 cgtgcggcgc attcgcggcc aagtggaagc gatcgaacgc gctctggagg ccgagactga
      481 atgcgcagcc gtgctgcagc tgatcgcctc cgttcgcggc gccatgaacg gcctaatggc
      541 cgaggtgatg gaagatcaca tccggatgca cgtcgttgat ccggccagcg accccgaccc
      601 tgaacgggct aggggcgcgg aagaactcat cgacgtcgtg cgcacctacc tgaaatgacc
      661 aggaatacga ccatggccga ccaattgaat cgtgatcgca ccaccctggc cgatcaggcc
      721 gaagcccgaa cgaccgcgcc gggggcgctg aacgagaaga agcacccgca tcacgggcac
      781 gaccctgatg atcacgacga tgaggacagc cacgaccacg agcatccgtt cgaatggcag
      841 gaggccttgc gcatcggctt ggtggcggtc gccgcggccc tcgtctggtt ccgggtctgg
      901 gagcccatcc ccactgttag cgtgatcggc gtcgtcggcc tcttgatcgg cggctggccg
      961 atcttgaagg aggcgttcga gaacattctg cagcggcgca tgacgatgga actgtcgatg
     1021 acaatcgcca tcgttgcggc tgccgccatc ggtcagttct tcaccgccct ggtgatcacc
     1081 ctcttcgtgt tggtggccga ggtcctagag ggcctgacgg tcgggcgagg gcgcaaggcc
     1141 atccgcgatc ttctggagtt cctgcctagc gaggtttccg tccgccgaac cggctccatt
     1201 cgaacgattt ccgctgccga gctcaatgtt ggcgacgcta tcctggtggc tccgggcgga
     1261 cgtatcccgg tcgatggcgc ggtcctgtcc ggcaactctt tcgtcgatga atcccgcatc
     1321 accggcgaat cgatgccggt ggagaagatc gaaggcgccg cagtgttcgc cgggtcgatc
     1381 aatcagtcgg gcgggcttga agtgcgcgcc gagcggatcg gacgcgacac aagctacggc
     1441 aagatcattg aggcggttga acgggccgag cgctcgcgcg cacccgtcca acgcctggcc
     1501 gaccgcatgg ccggctacct ggtctatttc gccctcggat cggcggtgct gacctatctg
     1561 atcacccaga acatcaactc aacgatctct gtgattatcg ttgccggggc ctgcggcatc
     1621 gccgccggca ccccacttgc gatcctcggc ggcatcggcc gctccgctcg gctcggcgcc
     1681 atcatcaaag gcggcgtcca tctcgaaacg ctcggcaagg tcgacaccgt ggtgctcgac
     1741 aagaccggca cgctcacatt cggtcggcca gaagtgcaag ccctcgtagc cgcgcccggc
     1801 gtcagcgccg aggatctgct cgacgcggct gccgcagccg agctgcgctc ggagcacccg
     1861 ctgggcaagg ccatcatcac ccatgccaag gccaaagggc gcccggttgc tgagcctgaa
     1921 cacttcgcct acacgcccgg tcggggcatc gccgccaggg tgacgggctc cttgatcctg
     1981 gtcggcaacc aggcctggat gcgggaaaat ggcgtggcga tcctagcgtc gctgaacttc
     2041 ggcgtcgagg cggcctccga ggtctatgtc gccagggacg gagcgctgct cggcgcgatt
     2101 acagtggccg acacggtgcg ccccgaagcc aagcgcgcga tcgaaagcct gacggctctt
     2161 gggatccgca ctctcctgct gaccggcgac gcgcttcccg ttgctcaggc tgtgggccgc
     2221 agcctcggca tcaaggaaat tgaagccagc ctcctgccgg aagacaagct cgcgcgaatc
     2281 caggcactca tcctggagaa gaggacggtt gccatggtcg gcgacggcgt caacgatgca
     2341 cccgcactgg ccgaagcgag cgtcggcatc gcgatgggtt ctgggacgga cgtcgcccag
     2401 gagagcgccg acatcgtgct gctgggcaat gatcttgtct gtctcgtaga gaccctcaag
     2461 gtggcaaggc gcacgcggcg catcatctgg cagaacttcg ccggcacgct cgcgatcgac
     2521 gcgatcggga taggcttggc ggctgtgggt attctaaatc ccctgatggc agcgttcatt
     2581 cacgttgcgt ccgagatggc cttcatcctc aattcagccc ggctgctacc gcgcaaggta
     2641 ggggctgacg acgggggggc gccgtcaacg ccttttggcg ttcagcccgc ggcggtctac
     2701 ccgtgaagtc gagcgatcca tgcatcagcg tatgcagttt tgatggtcga tcaggctggt
     2761 gcatcggctg tggtcgaacc gttcctgaaa tccgcgcctg gcggaagatg caaccccgtg
     2821 ctcgacagat ggt
//
LOCUS       DAOMEO010000336         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3880_length_2819_cov_2.581404, whole genome shotgun sequence.
ACCESSION   DAOMEO010000336 DAOMEO010000000
VERSION     DAOMEO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_2819_cov_2.581404"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLH31_08510"
     CDS             complement(<1..484)
                     /locus_tag="PLH31_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89291.1"
                     /translation="MKVLVACEYSGTVRNAFLNLGHDAWSCDLLPSEDGSNRHIIGDA
                     RDLLNEGWDLLMVAHPPCTRLCNSGVRWLTKPPPGKLIEEMWAELDEGAALFSDFWNA
                     PIERIAIENPVMHKHAKARIVNFQDFAQSIQPWQFGHGETKRTCLWLKNLPPLAPTEI
                     V"
     gene            723..1565
                     /locus_tag="PLH31_08515"
     CDS             723..1565
                     /locus_tag="PLH31_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HQR89292.1"
                     /translation="MSDPDGISGTDWSDNEQDLIVADYFDMLQIELAGGTVNKAERNR
                     ALQALTGRKRGSIEYKHMNVSAALMRIGLPTIDGYKPYPNIQDSLLDAIDRYLSSRPS
                     ILNIASSPLAEGFAEAPSLFAEPPPVRATGEPLVRKRMEALVRKFDPVERDFRNRALG
                     KAGEELVVLFEERRLHDGGRKDLAQQVRWVADEEGDGHGYDIESFALSGEKRLIEVKT
                     TTGGQTTPFFLTRNEERVSREREEFRLYRLYDFNKAPRLFKLRPPLGDSVVLETETWR
                     AGFG"
     gene            1708..2028
                     /locus_tag="PLH31_08520"
     CDS             1708..2028
                     /locus_tag="PLH31_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR89293.1"
                     /translation="MLCRYNRRATRDAVIAVLRAAEVALRGMGVSRAALFGSTARGEA
                     RPDSDLDIFVELDPAAKLDLYDYVGITQYLADLFTAPVDVVDRAGLKAHVRPSAERDA
                     VYAF"
     gene            complement(2075..2260)
                     /locus_tag="PLH31_08525"
     CDS             complement(2075..2260)
                     /locus_tag="PLH31_08525"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HQR89294.1"
                     /translation="MRREPTYDPEADAAYIKLAEGGYYESEEVSPGVILDFTQDGKLL
                     GIEILSASKRLARMTPG"
     gene            complement(2490..>2819)
                     /locus_tag="PLH31_08530"
     CDS             complement(2490..>2819)
                     /locus_tag="PLH31_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516459.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQR89295.1"
                     /translation="AEPVDALSMLVHRDRAESRGRGMCEKMKDLIPQHMFVIPIQAAI
                     GGKIIARETVRALRKDVTAKCYGGDASRKRKLLDKQKAGKKRMRQFGKVEIPQEAFIA
                     ALKMDED"
BASE COUNT          502 a          819 c          900 g          598 t
ORIGIN      
        1 cgacaatctc tgtcggcgcc aatggaggaa ggttctttag ccaaaggcat gtgcgctttg
       61 tttctccatg accgaattgc catggctgga tgctttgggc gaagtcttgg aagtttacga
      121 tgcgcgcctt cgcatgcttg tgcatcaccg ggttctcgat cgcgatgcgt tcgatgggcg
      181 cgttccagaa gtctgagaac aaagctgcgc cctcatcgag ttccgcccac atctcttcga
      241 tcagttttcc gggcggtggc ttcgtaagcc accgaacacc agaattgcag agtcgggtgc
      301 aaggcggatg ggcgaccatg agcaggtccc agccttcgtt cagaaggtcg cgagcatctc
      361 caatgatatg gcgatttgag ccgtcctcgc tgggcagaag gtcgcaagac catgcgtcgt
      421 ggcccagatt cagaaaggcg tttcggacgg ttccgctgta ctcgcaggcg accaagactt
      481 tcatgatcgc cgccggcgga aggtcgcgaa ctcccgattt gggcgcgggt ttgggagatt
      541 gaacatttgc acctagtcgc tctttagggt cccgctaagc gggtggttga gcgactatgg
      601 ctggaaaaat accaggataa gttgaatttt ttggatccgg ctcgactttt tgagcgggct
      661 tcttgccgga caaatactga gcgtactggt ttgtggctct gatccgagcc aggatcgtgg
      721 ctatgtcaga tcctgatgga atttctggga cggattggtc ggacaatgag caggatctca
      781 tcgtcgccga ttatttcgac atgttgcaga tcgaattggc cggcgggacc gtcaacaagg
      841 ccgaaagaaa tcgtgcgctt caggccttaa caggtcgaaa gcgcggttct attgaataca
      901 agcacatgaa cgtatccgcg gcgcttatgc ggatcggctt gccgactata gatggatata
      961 agccataccc gaacattcag gactcattgc tggatgcgat tgatcgatac ctttcaagtc
     1021 gtccctcaat tttaaatata gcgtcctcac ctttggcgga gggttttgcc gaagcgccaa
     1081 gtctgtttgc agaacctccg ccggttcggg cgactggcga accgctcgtt cggaagcgaa
     1141 tggaggcgct ggtgcgcaag tttgaccctg tagagcggga tttcagaaat cgcgcgcttg
     1201 gcaaggccgg cgaggagctt gtcgtcttgt tcgaagagcg ccggctgcac gatggcggcc
     1261 gtaaggatct tgcgcagcag gtgcgatggg tcgccgacga agagggcgat ggccacggct
     1321 atgatatcga gtccttcgcc ctttccggcg agaaacgact gatcgaggtc aagacgacga
     1381 ccggcgggca gacgacaccc ttctttctga cgcgcaatga ggagcgtgtt tcgcgcgagc
     1441 gagaggagtt ccgtctctac cggctgtacg atttcaacaa ggcgccgcgc ctgttcaagc
     1501 tgcggccgcc gctgggtgat agcgttgtct tggagacgga gacgtggcga gccggattcg
     1561 gctaaggcgg cagatgcgag ggctctgctc cgcttccctt ctccccttgc gggagaaggt
     1621 gtcagcgaag ctgacggatg aggggttgat catcctgtcc ggaaaagtct gggagccccc
     1681 tcacccgacc cgctactcgc gccccaagtg ctttgccgct acaatcgccg cgcgacccgt
     1741 gacgccgtca tcgccgttct gcgcgccgcc gaggtggctt tgcgcggcat gggcgtctcg
     1801 cgggcggcgc tgttcggctc gaccgcgcgg ggcgaggcgc ggcccgatag cgatctggac
     1861 atcttcgtgg agctggaccc cgccgcgaag ctcgatctct acgactatgt cggcatcacc
     1921 cagtatctcg cggacctgtt cacagctccg gtcgatgtcg tcgaccgcgc gggcctgaag
     1981 gctcatgtgc gaccatcggc cgagcgcgac gcggtctatg ccttctgagc gcgacgttca
     2041 ggccaagcct ccgtcgccgc ctattccgcc gcctctatcc gggcgtcatc ctcgccaggc
     2101 gcttgctggc gctgaggatc tcaatgccga gaagcttgcc gtcctgggtg aagtcgagga
     2161 tcacgccggg cgagacctct tcgctctcgt agtagccgcc ctcggccagc ttgatatagg
     2221 ccgcgtcggc ttccggatca taggtgggtt cacggcgcat cgggcggcct cgcgttccgg
     2281 tcgggaaaga ccgacaggac ccgaatatgg tccttttcct cgacgcacgc caccgcgccc
     2341 ccttctcccc ttgcgggaga aggtgtcagc gaagctgacg gatgaggggt tgaaggacga
     2401 ggccgccgcg tcacggctta ggccggtgca acccctcatc cgtcgcctga cggcgacacc
     2461 ttctcccgca aggggagaag gaagccagat caatcctcgt ccatcttcag cgcggcgatg
     2521 aacgcttcct gcgggatctc gaccttgccg aactggcgca tgcgcttctt gccggccttc
     2581 tgcttgtcca gcagcttgcg cttgcggctg gcgtcgccgc cgtagcactt ggcggtgacg
     2641 tccttgcgca gggcgcggac ggtttcgcgg gcgatgatct tgccgccgat ggccgcctgg
     2701 atggggatga cgaacatgtg ctgggggatc aggtccttca tcttctcgca catgccgcgg
     2761 ccgcggctct cggcgcggtc gcggtggacc agcatggaga gggcgtcgac gggctcggc
//
LOCUS       DAOMEO010000337         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3886_length_2816_cov_2.320898, whole genome shotgun sequence.
ACCESSION   DAOMEO010000337 DAOMEO010000000
VERSION     DAOMEO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3886_length_2816_cov_2.320898"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLH31_08535"
     CDS             complement(<1..189)
                     /locus_tag="PLH31_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89296.1"
                     /translation="MLVGILRSGALVAAGQTTLPQDGWRVSGSARARGETLSGQFRPT
                     AEPSDAVLTTRLTLAVERD"
     gene            519..>2816
                     /locus_tag="PLH31_08540"
     CDS             519..>2816
                     /locus_tag="PLH31_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89297.1"
                     /translation="MVLIALVRLALAKPLTFIVMALVILGLGVGAIARTPVDLFPRIG
                     VPVIAVAWSYNGLAPVDMAGRIITPFERVLTTTVNDIDRIESQSLTGVGVVKIYFQPG
                     VDVRTATAQVTSVSQTILRQLPPGITPPFIVNYDASTVPILQLALSGEGLTEQQLFDL
                     GLNQVRPQLVTIPGAAMPFPSGGRQRQIQIDIDPAALEARNLSAQDIAATLAAQTQIN
                     PVGFAKIGAYQYSLRLNNAPSSVEQLNELPIKVVDGATVLMRDVAQVRDGSAPQTNVV
                     HVDGQRSALMTVLKNGSASTLAVVESVKDRLPEAISGLPDALSVDLIGDQSALVEAAV
                     HTVVFEGFLAASLTALMILIFLGSWRSTVIIVVSIPLSILGSIILLAATGQTLNLMTL
                     SGLALAIGILVDDATVTIENINWHLEQGKPVTTAILDGGAQIVGPAFVALLCICVVFV
                     PMFFLPGVSGFLFVPLALAVVFAMVTSFLLSRTLVPTLAQYLLRPHRSDASQGRSLPA
                     RLQHGFEARFERMRQSYGRGLAWVLSRPRLVVPAFLVISLLSLGLTPLLGRDFFPAVD
                     SGQIALHVRAPAGTRIEETARLFADVQASVREVIGEAEIASVVDNIGLSSAPLNVIYA
                     NSGTVGPNEGDILIALAHGHGPTAEHVARLRNVLSDRFPEATFAFLPADMTTQILNFG
                     VPAPIDVQIRGRRILENAAYAQTLLREIRGIPGLVDARVQQSTRYPQLEIEVDRAQLA
                     QQGLTQRDVTSDLAIALAGTAQSAPV"
BASE COUNT          463 a          919 c          847 g          587 t
ORIGIN      
        1 atcccgctcg acggcaagcg tcagccgcgt cgtcaggacg gcgtcgctcg gctccgccgt
       61 cggacgaaat tggcccgaca gcgtctctcc ccgcgcgcgc gccgatccgg acacgcgcca
      121 gccgtcctgg gggagtgtcg tttggccagc ggcgacgagt gcaccgctcc tgaggatgcc
      181 caccagcact ggacaagcga tcccgcggct ggcgcgggcc tcttgatgtc tcccatcgtc
      241 gtttcctgct gcgttttgtc gcaacaggcc gggtcaagct taagtcagcc ttaaggtgac
      301 aacgaagctc cgatgtcggt cggcttgtcg taaccggtgc tgatctcggc ccgtagaggt
      361 ctgcacgacg agggcccgct gcaagcgaga ggccccaggt cgcgagccgc ctcggccgtg
      421 aaggactctt tctagacccc gctaagcttc acttaagaat ggagcggcct gggcagggcg
      481 cggagaatgc tcatgaccga aattgacaat gcgagcgcgt ggttttgatc gcgctggtta
      541 gattagcctt ggccaaaccg ttgacgttca tcgtcatggc tctggtgatc ctcggactcg
      601 gcgtcggcgc gatcgcgcgt acgcctgtcg atctgttccc acggatcggc gtgccggtca
      661 tcgctgtggc ctggtcttat aatggcttgg cgcccgtcga catggccggg cgcatcataa
      721 cccccttcga acgggtactg accaccaccg tcaacgatat cgatcggatc gaaagccagt
      781 ctttgaccgg tgtcggggtc gtgaagatct atttccaacc tggtgtggat gttcgcacgg
      841 ccacggctca ggtcacatcc gtttcgcaga cgatccttcg tcaactaccg ccagggatca
      901 cgcccccctt catcgtcaac tacgacgcat cgacggtccc cattctgcag ctggcgctgt
      961 cgggggaggg tttgaccgag caacaattgt tcgacctggg cctcaaccag gtccgaccgc
     1021 aactggtgac cataccggga gcggcaatgc cgtttccctc aggcggtcgt cagagacaga
     1081 tccagatcga tatcgatcct gcggcgctgg aagcccggaa tctctcggcc caggacatcg
     1141 cggcaaccct agccgcgcag acgcagatca atcccgtggg attcgccaaa atcggcgcct
     1201 atcaatacag tctaaggctc aacaacgcgc ccagttcggt cgagcagctc aacgagctcc
     1261 cgattaaggt cgtggatggc gccaccgtct tgatgcggga tgtcgctcag gtccgtgacg
     1321 gctcggcacc tcagaccaat gtcgtccatg tggatggtca gcgctcggcg ctcatgacgg
     1381 tcctgaagaa cggcagcgcc tcaacccttg ccgtggtcga gagcgtcaag gatcggcttc
     1441 ctgaggccat atcgggtctg ccggacgccc tgtccgtcga tttgatcggc gaccagtccg
     1501 ccctggtgga ggcggccgtc cataccgtgg tctttgaggg ctttctcgcc gcctcgctga
     1561 ccgctctgat gatcttgatc tttttggggt cctggcgctc gaccgtcatc atcgtcgtct
     1621 ccatccccct gtcgatcctg gggtcgatca tcctgctcgc ggcgacggga caaaccctca
     1681 atctcatgac gttgagcggc ctggccttgg cgatcggaat tctcgttgat gacgccaccg
     1741 tcacgatcga gaacatcaac tggcatctgg aacagggcaa acccgtgacg accgcgattc
     1801 tggacggtgg cgcccagatc gtcggtccgg ccttcgtcgc cctgctttgc atctgcgtcg
     1861 tcttcgtccc gatgttcttt cttcctgggg tttcgggctt cctcttcgtc cccctggcgc
     1921 tggccgtcgt cttcgccatg gtcacgtcct ttctcctgtc gagaaccctg gttccgacgt
     1981 tggcccagta tcttctccga ccgcaccggt cggacgcctc ccaggggcga tcgcttccag
     2041 cccggcttca gcacggcttt gaggcccgct tcgagcggat gcggcagtcc tacggcaggg
     2101 gtttggcatg ggttctgtct cggccgcggc tcgttgttcc tgcctttctc gtcatcagcc
     2161 tgctgtcgct tggcctgacg ccgctgcttg ggcgagactt ctttccggcc gtggattccg
     2221 gtcagatcgc gctccacgtc cgagcgccgg cgggcacgcg cattgaggaa accgcgcgac
     2281 tcttcgctga tgtccaggcg tccgttcggg aggtcatcgg ggaagcggag atcgcctccg
     2341 tggtcgacaa cattggactg tcgagcgcgc ccctgaacgt catctacgcc aactcgggaa
     2401 ccgtcggtcc aaacgaaggc gacatcctca tcgctctggc gcatggacac gggccgacgg
     2461 cggagcacgt cgcccgtctg cggaacgtct tgtcggaccg ttttcctgag gcgacgttcg
     2521 cgtttcttcc ggccgacatg acgacccaga ttttgaactt cggcgtacct gcgccgatcg
     2581 acgttcagat tcgcggacgc cgtattcttg agaacgccgc ctatgcgcag acgctcctgc
     2641 gcgaaatcag aggcatccct ggcctggtgg acgccagagt gcagcaatcg acgcggtatc
     2701 ctcaactcga gatcgaggtg gatcgcgcgc aactggccca gcagggcctg acgcagcgcg
     2761 acgtgacctc cgacctggcc attgccttgg ccggaacagc ccagtcagcg ccagtc
//
LOCUS       DAOMEO010000338         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3892_length_2810_cov_2.381125, whole genome shotgun sequence.
ACCESSION   DAOMEO010000338 DAOMEO010000000
VERSION     DAOMEO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_2810_cov_2.381125"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLH31_08545"
     CDS             complement(<1..318)
                     /locus_tag="PLH31_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HQR89298.1"
                     /translation="MRLLVVGKLNGQLSVAVKMAMNTGAKVSHVETIEQATHALRAGQ
                     GADLLMVDYSLDIAGLIAANESERIRVPVVACGVDADPMRAAAAIKAGAKEFIPLPPD
                     AELI"
     gene            complement(346..681)
                     /gene="fliN"
                     /locus_tag="PLH31_08550"
     CDS             complement(346..681)
                     /gene="fliN"
                     /locus_tag="PLH31_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HQR89299.1"
                     /translation="MSEDNLTLDEFGGQMLASEMPVEISDKIASDLAPVFDVPVNISA
                     VLGRAHMSVAQLLQLGQGSILELDRKVGEAIDIYVNNRLVARGEVVVVDERLGVTMTE
                     IIKDGDAAG"
     gene            complement(687..1337)
                     /locus_tag="PLH31_08555"
     CDS             complement(687..1337)
                     /locus_tag="PLH31_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HQR89300.1"
                     /translation="MTDIPHKRFAFDTVFDDGGGVAYQAPKVKRSFTPEEVEAAKAQG
                     YAEGERSAIARAEQHAANALAEISRAVHEAFSTLAAVAHEHRSGSALLALAAGRKIAD
                     AALERFPEAPATAALVALAREVEAQPRITVRVSEDLVERTQAALEETAHAIGFPGQIV
                     ARADNTMPPAAFTFDWGDGRASFDPDEANRRVAEALEAAIAAEGLHAEPLLPASES"
     gene            complement(1351..2330)
                     /locus_tag="PLH31_08560"
                     /pseudo
     CDS             complement(1351..2330)
                     /locus_tag="PLH31_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            complement(2330..>2810)
                     /locus_tag="PLH31_08565"
     CDS             complement(2330..>2810)
                     /locus_tag="PLH31_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HQR89301.1"
                     /translation="DAARGDQVKVTNIRFPQPEDQALGKDGLLSGFDKNDIMRIAELG
                     VLGIVAILILLFAVRPFLKNMMTPSPAQSALGAPQVTRMVTLSDGSTQEVVIDQSGEP
                     IAIAGPGTGIADIDQRIDIAKIEGQVKASSIKRVSEFVDKHPDESVAILRTWLHEST"
BASE COUNT          440 a          905 c          947 g          518 t
ORIGIN      
        1 gatcagctcg gcgtccggcg gcagcggaat gaattccttg gccccggcct tgatcgcggc
       61 ggcggcgcgc atcggatcgg cgtcgacccc gcacgccacg accggcaccc ggatccgctc
      121 ggattcgttg gccgcgatca ggcccgcgat atccagcgag tagtcgacca tcagcaggtc
      181 ggcgccctgg ccagcgcgca gggcgtgggt cgcctgctcg atggtttcga cgtgcgagac
      241 cttggcccct gtgttcatcg ccatcttcac ggcgacggag agctgtccgt tcagtttacc
      301 aacgacgaga agccgcatga tcttgtcctc cttgacgctt caggctcagc ccgctgcgtc
      361 gccgtccttg atgatttccg tcatggtcac gcccaggcgc tcgtcgacga cgaccacctc
      421 gccccgggcc accaggcggt tgttcacata gatgtcgatc gcctcgccga ccttgcggtc
      481 cagttcgagg atgctgccct ggcccagttg cagcaactgg gcgacggaca tgtgggcgcg
      541 accgagaacc gccgagatgt tgacgggaac atcgaacacc ggggccaggt cggaagcgat
      601 cttatcgctg atctcgacgg gcatttccga agccagcatc tggcctccga actcgtcgag
      661 cgtgagattg tcttcggaca tggcggtcag ctttcgctag caggaagcag gggttcggcg
      721 tggaggcctt cggcggcgat ggccgcctcg agcgcttcgg cgacgcgacg gttggcctcg
      781 tcgggatcga aggacgcccg cccgtcaccc cagtcaaagg tgaaggcggc gggcggcatg
      841 gtgttgtcgg ctcgggccac gatctgaccc gggaacccga tggcgtgagc ggtttcctca
      901 agcgccgcct gggtgcgctc gaccagatct tcggacaccc gcacggtgat gcggggctgg
      961 gcctcgacct cgcgggccaa ggccaccaga gcggcggtgg cgggcgcttc ggggaagcgc
     1021 tcaagggcgg cgtcggcgat cttgcggccg gcggccaggg ccaacaaggc cgagcccgag
     1081 cgatgctcgt gcgcgacggc ggccagggtc gagaaggcct catgcacggc gcgggaaatc
     1141 tcggccagag cattggcggc atgttgttcg gcgcgtgcga tcgccgagcg ctcgccctcc
     1201 gcatagccct gggccttggc ggcctcgacc tcttcgggcg tgaagctgcg cttcaccttg
     1261 ggcgcctggt aggccacgcc gccgccgtcg tcgaagacgg tgtcgaaggc gaaacgcttg
     1321 tgggggatgt cggtcattct gccatccgcc tcagtagatc agctcgtcgt cgctgcccga
     1381 gccggccagc atgatttcgc ccttggcggc caggtccttg gcgacctgga ccatggcgac
     1441 ctgggcctgg tcgacgtctt tcagacgcac cgggcccatg ctttccatgt cttcgcgcat
     1501 gatcttggcg gcgcgctccg acatgttgga gaagaacatc tcgcgcagct tgtccgaggc
     1561 gcccttcagg gccagggcca gctgttcctt gccggtggcg cgcagcaggg tctggacgcc
     1621 gcccgaatcc agcttcgaca ggtcttcgaa cacgaacata agcgcgcgga tgcgctcggc
     1681 ggcctcgcgg ttgcgctctt ccagggccgc gatgaagcgg gcctcggtct ggcggtcgaa
     1741 gttgttgaag atctcggcca tcatctcgtg gctgtcgcgc ttggacgtgc gcgccaggtt
     1801 cgacatgaat tcggtggctc catccgcagc atgcgggtca cgcattccag ggcgaagtct
     1861 tccggcagcg aggccagcac gcgggcggcg tggtcggact tcaccttgga cagcaccacg
     1921 gcgacggtct gggggtattc gttcttcaga tagttggcga gcaccgcctc gttcacgttg
     1981 cccagcttgt cccacatggt gcgacccgcg ggaccgcgga tctcttccat cagggcgtcc
     2041 accttttcag gcggcatgaa ggagttcagc aggcgctggg tctgctcgta ggagcccatg
     2101 atcgcgcccg tcgagctcat gcccgagacg aactcgacca gcaactcttc gacgacgctg
     2161 gccgacacgg tgccgaggcc cgccatggcc tgggagactt ccttgatctc ctcgtcatcc
     2221 agggcttccc agatgcgggt gtgatcctcg cccagcgaca gcagaacgat cgcggccttt
     2281 tccggcccgg acagtttgct gacgtcgttg atggaggtct tgatcgccat caggtcgact
     2341 cgtgcagcca ggtgcgcagg atggcgaccg actcgtcagg atgcttgtcg acgaattcgg
     2401 acacgcgctt gatcgacgag gccttcacct ggccctcgat cttggcgatg tcgatgcgct
     2461 ggtcgatgtc ggcgataccc gtgcccggac cagcgatggc gatgggctcg cccgactggt
     2521 cgatgacgac ttcctgggtc gaaccgtccg acagggtgac catgcgggtg acctgcggcg
     2581 cgcccaacgc tgactgggcc ggcgacggcg tcatcatgtt cttcaggaac ggacggacag
     2641 cgaacagcag gatcaggatg gcgacaatgc ccagaacgcc cagttcggcg atgcgcatga
     2701 tgtcgttctt gtcgaagccc gacagcaggc catccttgcc cagggcctga tcctcaggct
     2761 gcgggaagcg gatgttggtc accttgacct ggtcgccgcg ggcggcgtcg
//
LOCUS       DAOMEO010000339         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3897_length_2804_cov_2.592579, whole genome shotgun sequence.
ACCESSION   DAOMEO010000339 DAOMEO010000000
VERSION     DAOMEO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3897_length_2804_cov_2.592579"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLH31_08570"
     CDS             complement(<1..390)
                     /locus_tag="PLH31_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR89302.1"
                     /translation="MTYSDGEEIYAQDEETDMVYQVQRGAVRASRLLGDGRRQIAGFY
                     YPGDLFGLEAGPTHRASAEALCDSRVLVARRSALKHYGEAGERLERLIWRATGQELGR
                     CHDHMMLLARKSAYERVAGFLSDVAQRQ"
     gene            668..1465
                     /locus_tag="PLH31_08575"
     CDS             668..1465
                     /locus_tag="PLH31_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR89303.1"
                     /translation="MKTVLLLVHNDVGQEARLQAALDLTRALNGHLLCVDVTPLMTFA
                     GDIGGVAQGVILAEARKIEAANRIAIEARLAQEQAPWDWIDATGDVAQCLIREAGLAD
                     LIVVNCKRERLLDVEPHAVVSDVVTQARCPVIAVPDPVRGVDVTGLVVVAWDGSPPVM
                     ATLRASTPLLKLAASVELLTVGDHEGVPTEAAAAYLSRHGVHAAIRRIPANCDRPDTQ
                     ILEACAAAGAGYCLMGAYGHGRLKEELFGGVTRGMLDAARLPLLLGR"
     gene            complement(1413..>2804)
                     /locus_tag="PLH31_08580"
     CDS             complement(1413..>2804)
                     /locus_tag="PLH31_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQR89304.1"
                     /translation="LFDAAAALRAGQALAGDRLSIVTNGGGAGILALDAMASAGGDLA
                     ELGTETLTRLEAVLPAGWSHGNPVDLVGDASAERYRLALDIILSDPGVDAVLVMNCPT
                     ALSKPGDIAQGVAASVVAARGQGIVKPVIGCWLGDANQDAAGPILSAAGIPMFGAPDA
                     AARAFGHLLAVRRAKAGLMTAPAGHDPRADGRTVALALIQAARSEGRTLLNEVEAKTL
                     LSAYGVATVPTRLAAGIEDIAALCAELAPPYAVKIVSPQIVHKSDVGGVVLDLATAEA
                     AITAATEMRARIAKILPEATITGFAVGTMIPPGRAHELIVGLSQDATFGPVLMVGAGG
                     KAVEVLRDRALGLPPLDSERARAMISKTRISRLLAGYRDEPPADIEGVVGVVEALSAI
                     AVDLPDVVELDINPLRVDADSVLALDARVVITQEPSPPSGLVIQPALREQRAQSISDP
                     AAEEAAPHPACRA"
BASE COUNT          443 a          927 c          987 g          447 t
ORIGIN      
        1 ctggcgctgg gcgacgtccg acagaaatcc ggcgacgcgc tcataggcgc ttttgcgcgc
       61 caggagcatc atgtggtcgt ggcatcggcc cagttcctga ccggtggcgc gccagatcag
      121 gcgctccagg cgttcgcccg cctcgccata gtgcttcagg gccgagcgcc gggccacgag
      181 cacccggctg tcgcacagag cctcggccga ggcgcgatgc gtcggccctg cctcaaggcc
      241 aaacagatcg ccaggataat agaagccagc gatctgtcgg cgtccgtcgc caagcaggcg
      301 ggaggcgcga acggcgccgc gctggacctg atagaccatg tcggtctctt cgtcctgagc
      361 gtagatttcc tcgccgtcgc tgtaagtcat ggcgctgccg atcgcgccga agatctctgc
      421 ggatgtaatc ggttgagcgt acggtgtcgt aaaggcggcg cgatctggca gaaaagcggg
      481 catggacatc gcacgtctcc tcgagcggtg ggcctaaggg ataggtcaac ggccattggt
      541 cgcccattgg gatgtttacg tagtcgcggc caggaccgcg cgaacctgtc gttctccgta
      601 aaagtccgga tatcgaagcg ccgatcgcgc gcctagcgtt tcgacttcga aagccaggga
      661 ggcgaggatg aaaacggttc tactgcttgt gcataatgac gtgggccagg aggcgcggct
      721 gcaggcggcg cttgatctca cccgcgccct gaacgggcac ctgttgtgcg tcgatgtcac
      781 gcccctcatg acattcgccg gcgacatcgg cggcgtggcc cagggggtga tcctggccga
      841 ggcgcgcaag atcgaagcgg ccaatcgcat cgccatcgag gcgcgcctgg cccaggagca
      901 ggcgccctgg gattggatcg acgccaccgg cgacgttgcc caatgcctga tcagggaggc
      961 aggcctggcc gatctgatcg ttgttaactg caaacgcgag cgactgctcg acgtcgagcc
     1021 gcacgccgtg gtcagcgatg tcgtgaccca ggcccgttgc ccggtgatcg cggttccgga
     1081 cccggttcgt ggcgtcgacg tcaccggcct ggtggtggtc gcctgggacg gttcgccgcc
     1141 ggtgatggcg accttgcgcg ccagtacgcc gttgctgaag ctcgccgcct cggttgagct
     1201 cctgacggtg ggcgaccacg agggcgtccc gaccgaggcg gcggcggcct atctgtcgcg
     1261 ccatggcgtt cacgccgcca tccggcgcat tccggccaac tgtgaccgcc ccgacacgca
     1321 gattctcgaa gcgtgcgccg ccgccggcgc gggttactgt ctgatgggcg cctacggcca
     1381 tggacgcctg aaagaggagc tgtttggcgg tgttacgcgc ggcatgctgg atgcggcgcg
     1441 gcttcctctg ctgctgggtc gctgatcgat tgggcccgct gctccctgag ggcgggctgg
     1501 ataaccaagc ctgacggcgg gctgggctcc tgggtgatga cgacccgggc gtccagtgcc
     1561 agaacgctgt cggcgtcgac acgcagaggg ttgatgtcca gctccacgac atcgggcaga
     1621 tcgacggcaa tcgccgagag ggcctctacg accccgacca cgccctcgat atcggccggt
     1681 ggctcgtcgc gatagccggc cagcagccgc gaaatccggg tcttggagat catcgcgcgg
     1741 gcgcgttcgc tgtccagggg cggcaggccc agggcgcggt ctcgcaggac ctcgacggcc
     1801 ttgccgcctg cgccgaccat caataccggc ccgaaggtgg cgtcctggga cagtccgacg
     1861 atcagttcgt gcgcccggcc gggcgggatc atggtcccca ccgcaaaccc ggtgatcgtg
     1921 gcttccggca ggatcttggc gatgcgcgcg cgcatctcgg tcgcggcagt gatggcggcc
     1981 tctgcagtcg caagatcaag cacgacgccg ccaacgtcgg acttgtggac aatctgcggc
     2041 gagacgatct tgaccgcata gggcggtgca agctcggcgc agagtgcggc gatatcctca
     2101 atgcccgcgg ccaggcgggt cgggaccgtg gcaacgccat aggcgctcag cagggtcttg
     2161 gcctcgacct cgttgaggag cgttcggccc tccgatcgcg cggcctggat cagggccagg
     2221 gccaccgtcc ggccgtcggc gcgcgggtca tggccagccg gggccgtcat caggccagcc
     2281 ttcgcgcggc ggaccgccag cagatggccg aaggcccgag cggcggcgtc cggcgcgccg
     2341 aacatcggaa tcccggctgc gctgaggatc ggcccggcgg cgtcctggtt ggcgtcgccc
     2401 agccagcagc cgatcaccgg tttgacgatc ccctgtccgc gcgcggctac gacactggcg
     2461 gccacgccct gcgcgatatc gcccggcttc gacaaggcgg tggggcagtt catgaccagg
     2521 acggcgtcga ccccgggatc tgacaggatg atgtccagcg ccaggcgata gcgctccgcg
     2581 gaggcgtcgc ccaccaggtc gacgggattg ccatgcgacc aaccggcggg aaggacggct
     2641 tccaggcgcg tcagagtttc cgtgccgagc tcagccaggt cgccgccagc cgacgccatg
     2701 gcgtccaggg ccaggatgcc cgccccgccg ccattggtga cgatggacag tcgatcaccg
     2761 gccagggcct gccccgcgcg taacgccgcg gcggcgtcga agag
//
LOCUS       DAOMEO010000340         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3904_length_2799_cov_2.412901, whole genome shotgun sequence.
ACCESSION   DAOMEO010000340 DAOMEO010000000
VERSION     DAOMEO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3904_length_2799_cov_2.412901"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="aroB"
                     /locus_tag="PLH31_08585"
     CDS             complement(<1..1080)
                     /gene="aroB"
                     /locus_tag="PLH31_08585"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQR89305.1"
                     /translation="MIRTVSVGLGDRAYDVVIGTGLIDRAGEHIAPLLKRKRTAIVTD
                     SIVGEHHGERLSVSLERAGVAVDIIVLPPGEQTKSFAGLADLSDRLLALGLERGDMIV
                     AFGGGVIGDLAGFAASIYKRGIDFIQVPTTLLAQVDSSVGGKTAIDTPRGKNLIGAFH
                     QPRLVLADLDVLATLPGREMACGYAEVIKYGLLGDFTFFEWLESNVHAVLERDTEALV
                     RAVGRSVEMKAQIVAEDEKEAGRRALLNLGHTFGHAIEAEMGFGDALKHGEAVGVGMA
                     QAFRFSARQGLCSSQDAVRAEAAIKAAGLPTRMSDVRPEPFLAEALIAHCGQDKKAEG
                     GTLTFVLARGIGEAFVAKDVGRAALR"
     gene            complement(1077..1646)
                     /locus_tag="PLH31_08590"
     CDS             complement(1077..1646)
                     /locus_tag="PLH31_08590"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQR89306.1"
                     /translation="MTVDPASSLQPLRNRTIVLVGLMGVGKSSVGRRLAQALDMPFRD
                     ADTEVENAAGRSIPEIFAELGEAAFRDGERRVIARLLEEPPHVLATGGGAFVNDETRS
                     LINQHAISVWLKADVELLARRVGRKDNRPLLKNRDPVEVLTQLAEVRYPAYAQAHVHV
                     ETGDTPHAVAVDAVISALRNRLDQESFQP"
     assembly_gap    1755..1764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1789..>2799
                     /locus_tag="PLH31_08595"
     CDS             1789..>2799
                     /locus_tag="PLH31_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89307.1"
                     /translation="MRSFPRRAAAPLLAAWLTGVAAPALAQVEVQSLAPPDLFSTPAA
                     QTDLGPDLWKDTAPDIARDVLPKLAARPLSPAFADLARRVLATGANGPTGVGADPDLG
                     AARAMALIALGEAVGADAVLDRAPGVANSAALSLAAAEAALIKGDDDKACRIGEALTI
                     DRGASYWLRLRAFCQARADEATAQLTFSLAQQQTKDADYARLMSAVLAGTPSGAANLR
                     NGINYALSRKLGLDIQAAAATASPALQPVIQPRADLSAIGLGADLTAAETSDLAFLRQ
                     AKGLPAFIETARTSSASIRTLAQADAPLRDPVLFARAALAAGDLPTAQLIRSRLTQDQ
                     IPG"
BASE COUNT          404 a          993 c          964 g          428 t
ORIGIN      
        1 ccgcagcgcc gcgcggccca cgtccttggc cacgaaggcc tcgccgatgc cgcgcgccag
       61 tacgaaggtc agggtcccac cttcagcctt cttgtcctgg ccgcagtggg cgatcaacgc
      121 ttccgccagg aacggctccg gtcgcacatc gctcatccgc gtgggcagac cggcggcctt
      181 gatggcggct tcggcgcgga cggcgtcctg gctggagcac aggccctggc gggccgagaa
      241 ccggaaagcc tgggccatgc cgacgccgac ggcctcgccg tgtttcagcg cgtcgccaaa
      301 acccatctcg gcctcgatgg cgtggccgaa ggtgtggccg aggttcagca gagcccggcg
      361 gccggcttcc ttttcgtcct cggccacgat ctgggccttc atctcgacgc tgcggcctac
      421 ggcgcggacc agggcctcgg tgtcgcgctc cagcaccgcg tggacgttgc tctccagcca
      481 ttcgaagaaa gtgaaatcgc ccagcaggcc gtacttgatc acctcggcat agccgcaggc
      541 catctcgcgg ccgggcaggg tggccagcac gtccagatcg gccagcacca ggcgcggctg
      601 gtggaaagcg ccgatcaggt tcttgccgcg cggcgtgtcg atggcggtct taccgcccac
      661 cgagctgtcc acctgggcca gcagggtcgt gggaacctgg atgaagtcga tgccgcgctt
      721 gtagatcgag gccgcaaagc cggcgaggtc accgatcacc ccgccgccga acgccacgat
      781 catgtcgccg cgctccagcc ccagggccag caggcggtcc gacaggtcgg ccagacccgc
      841 aaagctcttg gtctgctcgc caggcggcag cacgattatg tcgacggcga cgccggcccg
      901 ttccagcgag accgacagcc gctcgccatg atgctcgccg acgatggaat cggtgacgat
      961 ggcggtgcgc ttgcgcttca gcagcggggc gatgtgctcg ccggcgcggt cgatcagccc
     1021 cgtgccgatg acgacatcat aggcgcggtc gccaagcccg acggaaacgg tgcggatcat
     1081 ggctgaaaag actcctggtc aagacgattg cgcagggccg agatcacggc gtccaccgct
     1141 acggcatgcg gcgtgtcgcc ggtctccacg tggacatggg cctgggcgta ggcgggataa
     1201 cgaacctcgg ccagctgcgt cagcacctcg accggatcgc ggttcttgag cagggggcgg
     1261 ttgtccttgc ggcccacccg acgcgccagc agctcgacat cggccttcag ccacaccgag
     1321 atggcgtgct ggttgatcag cgagcgggtc tcgtcattga caaaggcgcc cccgccggtg
     1381 gccagcacat gcggcggctc ttccagcagg cgggcgatga cccgacgttc gccatcgcga
     1441 aaggcggctt cgcccagttc ggcgaagatc tcggggatcg accggccggc ggcgttttcg
     1501 acttcggtgt cggcgtcgcg gaacggcatg tccagcgcct gggccagccg ccggcccaca
     1561 ctggacttgc ccacgcccat caggcccacc agcacaatgg tgcggttgcg cagcggctgg
     1621 agcgaagagg cgggatcgac ggtcatgatg cggtcgcggg tttacacgag gctggggcct
     1681 agcgccaagt ttcgctgaac cgagttttgt tgagccccgt tttgccaagc ccggttttgc
     1741 ttggtggggg gcttnnnnnn nnnncataac gggagcatga tgtctcgcat gcgttctttt
     1801 cctcgccggg ccgccgcgcc cctccttgcc gcctggctga ccggcgtcgc cgccccggcc
     1861 ctggcccagg tggaggtgca gtccctggcg ccgccggacc tgttctccac ccccgccgcc
     1921 cagaccgacc ttgggcccga tctgtggaag gacaccgctc ccgacatcgc ccgtgatgtg
     1981 ctgccgaagc tggccgccag gccgctgtcg cccgccttcg ctgatctggc ccggcgggtg
     2041 ctggccaccg gcgccaatgg gccgaccggc gtgggcgccg accctgatct gggcgcggcg
     2101 cgcgccatgg ccctgatcgc cctgggcgag gcggtgggcg cggacgccgt gctcgaccgc
     2161 gcgccgggcg tggccaacag tgccgccctg tcgctggcgg cggccgaggc ggccctgatc
     2221 aagggcgacg acgacaaggc ctgccggatc ggcgaggccc tgaccatcga ccggggcgcc
     2281 agctactggc tgcggctgcg ggccttctgc caggcgcggg ccgatgaggc cacggcccag
     2341 ctgaccttct ccctggccca gcagcagacg aaagacgccg actatgcccg cctgatgagc
     2401 gcggtgctgg ccggaacccc gtcgggcgcg gccaatctgc gcaacgggat caactatgcc
     2461 ctgtcgcgca agctggggct ggatatccag gcggccgccg ccaccgcctc gccggcgctg
     2521 cagccggtga tccagccgcg cgccgacctg tcggcgatcg gcctgggcgc cgatctgacg
     2581 gcggccgaga cctcggacct ggccttcctg cgtcaggcca aggggctgcc ggccttcatc
     2641 gagacggcca ggacgtcttc ggcgtcgatc cggaccctgg cccaggccga tgcgccgttg
     2701 cgagatccgg tgctgttcgc gcgggcggcc ctggcggccg gcgacctgcc caccgcccag
     2761 ctgatccgct cgcgtctgac ccaggaccag attcccggc
//
LOCUS       DAOMEO010000341         2797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3905_length_2797_cov_3.281182, whole genome shotgun sequence.
ACCESSION   DAOMEO010000341 DAOMEO010000000
VERSION     DAOMEO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3905_length_2797_cov_3.281182"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1118)
                     /locus_tag="PLH31_08600"
     CDS             complement(15..1118)
                     /locus_tag="PLH31_08600"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQR89308.1"
                     /translation="MPDDVSPPPKASPATKFGEGFVMDAWYVAALSPDLAPGKMEHRE
                     FLAEPVLLGRTKAGVAYAIRDICPHRAAALSAGKVRTEADGAESIECPYHGWRFGTDG
                     ACTAIPSLTADSDLDISKVRVRRYPIVEQQGLVWIWMSSDARFDGEPPEAPPVIPGVV
                     GGKPKLVDHLDYDIHIDHAVLGLIDPAHGPYVHQQWWWRTTASQHEKTKRFAPSEAGF
                     TMVRHEPSKNSKAYAILGGEPLTEITFRLPGLRWEHVTVGQKQVLALSAMTPINANKT
                     RMNQIMWSDHWAFTAFYPIIRIAARAFLRQDGFIVGVQSQGLQSNPPLMWVGDADQQA
                     RWYQQCKREWQASRAEGRAFVNPVKETTLRWRT"
     gene            complement(1182..>2252)
                     /locus_tag="PLH31_08605"
     CDS             complement(1182..>2252)
                     /locus_tag="PLH31_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HQR89309.1"
                     /translation="SEASWTHAPFAGVVADGAVWGRGAIDDKGSLVTLFEAIEGLAAG
                     GFKPVRTVIVVSGHDEEVRGVGARAAAALLKSRGIKAQFVLDEGMVIVADHPVTGKPA
                     AIIGTAEKGYATMKIVAPAVGGHSSAPPEETGVATLSRAVLAITGKPFPLKFAGPGAD
                     MLKSIAPQAGLPVKVLAANTWLFAPLLVNITAKTPAGAAMLHTTIAPTMLKGSPKENV
                     LPQDATAWINYRIAPGDSSAKVMGVAKDAVGDLPVELSWVKTPDEPSRVSSTSSDAWK
                     TIAGLAADESKAPVVPGLVTAGTDSRYLAPVADDVYRFQPLVLSINDTKMIHGTDEHI
                     SVANVERMVRFYQRLVETAAGR"
     assembly_gap    2253..2262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2263..2641)
                     /locus_tag="PLH31_08610"
                     /pseudo
     CDS             complement(2263..2641)
                     /locus_tag="PLH31_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516146.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
BASE COUNT          407 a          929 c          979 g          472 t
ORIGIN      
        1 ggccctggcg gcgatcaggt ccgccagcgc agcgtcgttt ccttgaccgg attgacgaag
       61 gcccggccct cggcgcggct ggcctgccat tcgcgcttgc actgctggta ccagcgggcc
      121 tgctggtcgg cgtcgcccac ccacatcagc ggcgggttgc tctgcaggcc ctggctctgg
      181 acgccgacga tgaagccgtc ctggcgcaga aaggcccggg ccgcgatccg gatgatcgga
      241 tagaaggccg tgaacgccca gtgatcggac cacatgatct ggttcatgcg ggtcttgttg
      301 gcgttgatcg gcgtcatggc cgacagggcc agcacctgct tctggcccac cgtcacatgc
      361 tcccagcgca ggccgggcag gcggaaggtg atctcggtca ggggctcgcc gcccaggatg
      421 gcataggcct tggagttctt cgacggctcg tgccgcacca tcgtgaagcc agcctccgac
      481 ggggcgaagc gcttggtctt ttcgtgctgg ctggcggtgg tgcgccacca ccattgctga
      541 tgcacatacg ggccgtgcgc cgggtcaatc aggcccagca ccgcgtggtc gatatggatg
      601 tcatagtcca ggtgatcgac cagcttgggc ttgccgccca cgacgcccgg aatgaccggc
      661 ggcgcttccg gcggctcacc gtcgaagcgg gcgtccgacg acatccagat ccagaccaga
      721 ccctgctgtt ccacgatcgg atagcggcgc acccgcacct tggagatgtc caggtcgctg
      781 tcggcggtga gcgacgggat cgccgtgcag gcgccatccg tgccgaaccg ccagccgtga
      841 tacgggcatt cgatgctctc ggcgccatcg gcctcggtcc tgaccttgcc ggccgacagg
      901 gcggcggcgc ggtgcgggca gatgtcccgg atggcgtagg cgacgccggc tttcgtgcgg
      961 cccagcagca cgggttcggc caggaactcg cggtgctcca tcttgccggg cgcaaggtcc
     1021 ggcgacaggg ccgcgacgta ccaggcgtcc atgacgaagc cctcgccgaa cttggtggcg
     1081 ggagaagcct tggggggggg cgagacgtcg tcgggcatgg cggccttata gcccagttcg
     1141 ttgacttttc acgacacctt gcgcgcccgt cgccggccgc cttaccgtcc cgccgccgtc
     1201 tcgaccagtc gctgatagaa ccgcaccata cgctccacat tggcgacgct gatgtgctcg
     1261 tcggtgccat ggatcatctt ggtgtcgttg atcgacagca ccagcggctg gaagcgatag
     1321 acgtcgtcgg ccaccggggc caggtagcgg ctgtcggtgc cggcggtgac caggccgggc
     1381 acgacggggg ccttgctctc gtcggcggcc aggccggcta tggtcttcca ggcgtccgag
     1441 ctggtcgagg acaccctgga cggctcgtcg ggcgttttca cccatgacag ttccaccggc
     1501 aggtcgccga ccgcgtcctt ggcgacgccc atcaccttgg ccgaactgtc gccgggcgcg
     1561 atgcggtaat tgatccaggc ggtggcgtcc tggggcagga cgttctcctt gggcgacccc
     1621 ttcagcatgg tcggggcgat ggtggtgtgc agcatggccg cgccggctgg cgtcttggcg
     1681 gtgatgttca ccagcagtgg cgcgaacagc caggtgttgg ccgccagcac cttgaccggc
     1741 aggccagcct gcggtgcgat cgatttcagc atgtctgcgc cggggccggc gaacttcaac
     1801 gggaagggct tgcccgtgat ggccagaacg gcccgcgaca gggtcgcgac acctgtttcc
     1861 tccggcggcg ccgacgaatg tccgcccacg gccggagcga cgatcttcat cgtggcatag
     1921 cccttttcgg cggtgccgat gatggcggcg ggcttgccgg tcacggggtg gtcggcgacg
     1981 atcaccatgc cttcgtcgag cacgaactgg gccttgatcc cgcgagattt cagcagggcc
     2041 gccgcagccc gagccccgac gccgcgcacc tcctcgtcat ggccgctgac cacgatcacg
     2101 gtacgaacgg gcttgaagcc gcccgccgcc agaccctcga tcgcctcgaa cagggtgacc
     2161 agcgagccct tgtcgtcgat cgcgccgcga ccccacaccg cgccatcggc cacgacgccg
     2221 gcaaagggag cgtgggtcca ggaggcctcg ctnnnnnnnn nnggtgggcc atcagcacga
     2281 tcgggggcag ggcggggttc gagccggccc aggtatagac cagggtgtgg ccggtcagca
     2341 cctcgcgggt catggcggca tgggcggcgg gataggtggt ctgcagccag gcgtgcagcc
     2401 tgtcccattc tgccggcagg tcgtcggcct ggtcctggtg gctgatggtc tggaagcgga
     2461 tcgcctgggc caggttggcg gcggcccggg ccgtgtcgac cggcttggcg gcgaccaggt
     2521 tcgcggcggc cacgtcaacc gaagccggcg ccttgaaggt cgctgttcgc accgccacca
     2581 cgccggcgac ggccagcacc agtcctacgg ccgtcagccc ggccttgccc gcaaaaccca
     2641 tcacgcgtct cccccaaaca tttgttcggg actgtgcggc gggcgcggcg ctttgccaat
     2701 cctccgtttt gagagaggtg cgacccggct tcggcgtcgt cggggcttgc ctgagctagg
     2761 tcagggccgt gaagatcagg gtcaccacgg cgatgtc
//
LOCUS       DAOMEO010000342         2796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3909_length_2796_cov_2.260489, whole genome shotgun sequence.
ACCESSION   DAOMEO010000342 DAOMEO010000000
VERSION     DAOMEO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3909_length_2796_cov_2.260489"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1611
                     /locus_tag="PLH31_08615"
     CDS             30..>1611
                     /locus_tag="PLH31_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR89310.1"
                     /translation="MDAGRAASMLGGTLVAASVLSIAFTGWSLALYPYGDAYQQSHVA
                     FYMAITVIACIFCLMHLRAAALVVTLVVMGSMTVFFGLSGNPVFQAISVNLVVVAAVM
                     IVILLIHYKEFADLVESRTALIEKAQQAQLLSDENNRLANLDSLTGLPNRRRFFTELE
                     AHVTRARRHDRTLVVGVIDLDGFKPINDAFGHATGDRLLTEVGRRLQAFGSPPMFAAR
                     LGGDEFGLIIDAPLSPAALRILGRDLCELLRAPYRLPGVTAQIAGSIGFCPFPAGGQT
                     SEQLFERADYALYHAKQNLKGSAVVFDERHEADIRELGRVEQALRRADLDAEMHIHFQ
                     PIIDTGAKRTIAFEALARWSSPELGDVPPAVFINAAERSGQITEITRVLVAKALRAVN
                     TWPMDMRVSINLSARDIASMETVARIVDIVLESQVAPHRIDFEITETAIVCDFDQARA
                     ALMTFHALGVHTALDDFGTGHSSLSHVRLLPLDKLKIDASFISDIEHHAASEDIVKTV
                     LQLCRNLRIDCIVEGVETQ"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1622..1822
                     /locus_tag="PLH31_08620"
     CDS             <1622..1822
                     /locus_tag="PLH31_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89311.1"
                     /translation="QLDKVASLGGVVMQGYHFARPMPAASVAGYLAEEAAIAIARNGV
                     KQSAAQRAARIWFDEDGLQGLG"
     gene            complement(1881..2378)
                     /locus_tag="PLH31_08625"
     CDS             complement(1881..2378)
                     /locus_tag="PLH31_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89312.1"
                     /translation="MTSRAELLAAPEAVLRIVIEQGEKRLAGQIAFADSQDQRGGAII
                     NAAVALAAAAVALGAANITKPSLFVGSVIAVTGFSAAASIAMWALRSRGFHSLGYYPS
                     DFNDDIIEGKTVKDLLVDYAQELQHRLSENKPILDERGTLTNLATWALLGTPIGALIA
                     AYIRA"
     gene            complement(2626..>2796)
                     /locus_tag="PLH31_08630"
     CDS             complement(2626..>2796)
                     /locus_tag="PLH31_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQR89313.1"
                     /translation="VPGRLAMVVGDLRVVFDTHEEGVAPGQACVLYDPADPDRVLGGG
                     FIASTVRAELAV"
BASE COUNT          490 a          937 c          841 g          518 t
ORIGIN      
        1 tgggctggtg gcggttacgt cggaacggca tggacgccgg ccgcgccgcc agcatgctgg
       61 gcgggacctt ggtcgcggcc agcgttctgt caatcgcctt caccggctgg tcgctcgcgc
      121 tctatcccta tggcgacgct tatcagcaga gccatgtagc cttctacatg gccatcaccg
      181 tcatcgcctg catcttctgc ctgatgcacc tgcgggcggc ggcgttggtc gtcaccctgg
      241 tcgtgatggg gtcgatgacc gtgttcttcg gactgagcgg caatccggtt ttccaggcca
      301 tctcggtcaa tctggtggtg gtcgcggccg tgatgatcgt catcctgctg atccactaca
      361 aggaattcgc cgatctggtg gagtcgcgga ccgccctgat cgagaaggcc cagcaggcgc
      421 agttgctctc ggacgagaac aatcgcctgg ctaatctgga cagcctgact ggcctgccca
      481 accgccgccg cttcttcacc gaactcgaag cccatgtcac ccgcgcccgg cgtcatgacc
      541 gcaccctcgt ggtgggggtc atcgacctgg acggcttcaa gccgatcaac gacgcctttg
      601 gccacgccac gggcgaccgt ctgctgaccg aggttggccg tcgcctgcag gccttcggtt
      661 cgccgccgat gtttgcggcc cggctcggcg gcgacgaatt tggcctgatc atcgacgccc
      721 ccctgtcgcc ggctgcgctg cggatcctgg gtcgcgatct gtgcgagcta ctgcgcgcgc
      781 cctatcgcct gcctggcgtt accgcccaga tcgccggctc cattggcttt tgcccattcc
      841 cggccggggg acagaccagc gagcagctgt tcgagcgcgc tgactacgcc ctctatcacg
      901 ccaagcagaa cctgaagggt tcggcggtgg tgttcgatga gcgccatgag gccgacatcc
      961 gcgagttggg tcgggtggag caggccctgc gccgcgccga tctggacgcg gagatgcaca
     1021 tccacttcca gccgatcatc gacaccggcg ccaagcgcac catcgcgttt gaggccctgg
     1081 cgcgctggtc cagtccggaa ctgggcgatg tgccgccggc cgtgttcatc aacgccgccg
     1141 agcgttcggg ccagatcacc gagatcaccc gcgtgctggt cgccaaggcc ctgcgcgctg
     1201 tcaacacctg gcccatggac atgcgggtct cgatcaatct gtcggcccgt gacatcgcct
     1261 ccatggagac ggtagcccgc atcgtcgaca tcgtgctgga aagccaggtc gcgccgcacc
     1321 gcatcgattt cgagatcacc gagacggcca tcgtctgcga cttcgaccag gcccgcgccg
     1381 ccctcatgac cttccacgcc ctgggcgtac acaccgccct tgacgatttc ggcaccggcc
     1441 attccagcct cagccacgtg cgcctgctgc cgctggacaa gctgaagatc gacgccagct
     1501 tcatctccga catcgagcac cacgcggcca gcgaagacat cgtcaagacc gtgctgcaac
     1561 tgtgtcgcaa cctgcgtatc gactgcatcg tcgaaggggt cgagacccag gnnnnnnnnn
     1621 ncagctcgac aaggtggctt cgctgggcgg ggtcgtcatg cagggctacc acttcgcccg
     1681 cccgatgccg gccgccagcg tcgccggcta tctggccgag gaagccgcca tcgccatcgc
     1741 ccgcaatggc gtcaagcaga gcgccgccca gcgcgccgcg cgcatctggt ttgacgagga
     1801 tgggctgcag gggctggggt agggcggaga ggtccggctt cgtcgagctg cattctcgca
     1861 gggcagccct tcagaacgag ctacgccctt atgtaggcag cgatcagcgc gccgattggt
     1921 gttcctagta gagcccaagt cgccaaattc gtgagggttc cacgctcgtc gaggataggc
     1981 ttgttctctg acaagcggtg ctgaagctct tgcgcgtagt caactaacag gtctttcacc
     2041 gtcttgcctt cgatgatgtc gtcattgaaa tcgctggggt agtaaccgag agagtggaag
     2101 ccgcgagatc gaagcgccca catggcaatg cttgctgcgg cgctaaatcc ggtaactgct
     2161 atgaccgacc ctacaaacaa cgacggtttg gtaatatttg ctgcacccag cgcaacggca
     2221 gcagcagcaa gtgctacagc cgcatttatg atcgcgccgc cacgttgatc ctgcgagtca
     2281 gcaaaggcga tttgccctgc gagccgtttc tcgccttgct ctattacgat tctcagtaca
     2341 gcttcaggtg cagctaaaag ctcggcgcgg ctagtcaacg gctctccctt tcggtcagca
     2401 cgcctctgtc ctgctgaaaa cagctttcaa cgaaagagga gggagcgact tggcacctac
     2461 accgggtaac agaccaccag atcggcgatc gcctgagact aacacaccac gcccgtcgcc
     2521 tgcacctcca aggcccgcgc ctgcgccacc atctcggcca acgccgaagc gataacaaat
     2581 gggctatgct gacggcgctg attgactgta gcgccgtcag tgatcctaaa ccgccagttc
     2641 cgccctcacg gtgcttgcga taaacccgcc ccctagcacc cggtccggat cggcgggatc
     2701 gtagagcacg caggcctggc ccggcgcgac gccttcctcg tgggtgtcga acaccacgcg
     2761 caggtcgccg acgaccatgg ccaggcggcc cggcac
//
LOCUS       DAOMEO010000343         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3910_length_2795_cov_2.495620, whole genome shotgun sequence.
ACCESSION   DAOMEO010000343 DAOMEO010000000
VERSION     DAOMEO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_2795_cov_2.495620"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLH31_08635"
     CDS             complement(<1..576)
                     /locus_tag="PLH31_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR89314.1"
                     /translation="MTQIPVQPPIQTFGELDHPPRLLMGPGPINVHPRVLRAMSVQLL
                     GQFDPEFTGYMNETMALYRQVFETQNRWTFLIDGTSRAGIEAAMVSVLAPGDDVVILN
                     AGRFGLLLSEIAERCDARVTLVEGEWGKVIDAQAVEDAVARVKPRLVACVHGDTSTTM
                     AQPIEAIGEICRRHDALLYVDATATLSGMACP"
     gene            complement(573..1829)
                     /locus_tag="PLH31_08640"
     CDS             complement(573..1829)
                     /locus_tag="PLH31_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HQR89315.1"
                     /translation="MTGDFEIGARARARCDLLGAPPYSEVEGQLTRRFLTPAHNAALE
                     RLTLWMTSAGMTVRRDAAGNLVGRYEGETPNAPALMIGSHIDSVRNGGRYDGPLGIML
                     GIDVVEAQRDASRRLPFAIEVIAFGDEEGSRFPASMSCSRAIAGTLDPAAVLGMTDAE
                     GVTLADAFTAFGLDPDRITDAARKPVEALAFLEAHIEQGPVLEAEGLALGVVTAIAAQ
                     KRLMVRITGMAGHAGTTPMALRKDPGPAAAECILALERLCQAGTDNLVGTVGRLTALP
                     GAFNVIPGAVEFSMDIRAETSATRDAAVEAVTAEIHAIAARRGLSASVTLMQALSESP
                     CDPGLTAMLEAAVGHLGLPPRRLPSGAGHDAMVMAALCPTAMLFIRCEGGISHNPAES
                     VTEADCALAAQAMLHFIDKLERRERA"
     gene            complement(2008..>2795)
                     /locus_tag="PLH31_08645"
     CDS             complement(2008..>2795)
                     /locus_tag="PLH31_08645"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQR89316.1"
                     /translation="HEATLALKNYVVAALILVSRSMTDALPPVSDEDRPLAGEYVLGV
                     LDAAERAAVQSRIAAEPSFARAVEWWENRLTPLASDIRGVEPSAGLWPRIANLIGGAS
                     KPTLWNNARVWRGVSVGAVALAAASVAALVLTPRAPSPSVIAPAAPAPAEVALIKDPG
                     TNQAALVATLDHATDTLILTPVAMTMPEGREPELWIIPEGQKAISLGMLPTDAPRAIP
                     LPKDLRGAGAYTALLAVTDEPPGGAPTGVATGSVRAAGKFAKT"
BASE COUNT          400 a          921 c         1051 g          423 t
ORIGIN      
        1 tgggcaggcc atgcctgaga gggtggcggt ggcgtcgaca tacagcaggg cgtcgtggcg
       61 gcggcagatc tcgccgatgg cctcgatcgg ctgggccatg gtggtggagg tgtcgccatg
      121 cacgcaggcc accaggcgtg gcttgacccg ggccacggcg tcttcgaccg cctgcgcgtc
      181 gatgaccttg ccccattcgc cctcgaccag ggtgacgcgg gcgtcgcagc gctcggcgat
      241 ctccgacagc aacaggccaa agcggccggc attgaggatc accacgtcgt cgcccggcgc
      301 cagcaccgaa accatcgccg cctcaatccc ggcgcgcgag gtgccgtcga tcaggaaggt
      361 ccagcggttc tgggtttcga agacctggcg atacagcgcc atggtctcgt tcatgtagcc
      421 ggtgaattcc gggtcgaact ggcccagcag ctggacggac atcgcgcgca gcacgcgcgg
      481 atgcacgttg atcgggcctg ggcccatcag caggcggggc gggtggtcga gctcgccgaa
      541 ggtctggatg gggggctgga cggggatctg ggtcatgcgc gttcgcgtcg ttctagcttg
      601 tcgatgaaat ggagcatggc ctgggcagcg agggcgcaat cggcctctgt cacggactcg
      661 gccgggttgt ggctgatccc gccctcgcag cggatgaaca gcatggcggt ggggcacagg
      721 gcggccatca ccatggcgtc gtggcccgcg ccgctgggca ggcggcgggg cggcagaccg
      781 aggtggccga ctgccgcttc cagcatggcg gtgaggccgg gatcgcaggg gctttcggaa
      841 agcgcctgca tcagcgtgac cgacgcgctc aggccgcgtc gggcggcgat ggcgtggatc
      901 tcggcggtga cggcttcgac ggccgcgtcg cgcgtcgccg aggtctcggc gcggatgtcc
      961 atcgagaact cgaccgcgcc ggggatgacg ttgaaggcgc cgggaagggc ggtgaggcgg
     1021 cctacggtgc cgaccaggtt gtcggtcccg gcctggcaga ggcgctccag ggccaggatg
     1081 cactcggcgg cggcggggcc gggatccttg cgcagggcca tgggtgtggt gcccgcgtga
     1141 ccggccatgc cggtgatccg caccatcagg cgcttctggg cggcgatggc ggtgacgacg
     1201 cccagggcca ggccttcggc ctccagcacc gggccttgct cgatgtgcgc ttcgaggaaa
     1261 gcgagcgctt cgacgggctt gcgggccgcg tcggtgatcc ggtctgggtc caggccaaag
     1321 gcggtgaacg catcggccag ggtcacgccc tccgcgtcgg tcatgcccag cacggccgcc
     1381 gggtccagcg tcccggcaat ggcgcggctg cagctcatcg aggccgggaa gcgcgagcct
     1441 tcctcatcgc caaacgcgat cacctcgatg gcgaacggca ggcggcggct ggcgtcgcgc
     1501 tgggcctcga ccacgtcgat gcccagcatg atccccagcg ggccgtcata gcgcccgcca
     1561 ttgcggacgc tgtcgatatg agagccgatc atcagggccg gggcgtttgg agtctcgccc
     1621 tcgtagcggc cgaccagatt gccggcggcg tcgcgacgga cggtcatgcc ggctgacgtc
     1681 atccacaggg tcaggcgttc gagcgcggcg ttatgggcgg gggtcaggaa ccggcgggtc
     1741 agctggccct cgacctcgct atagggcggg gcgcccagca ggtcgcagcg ggccctggcc
     1801 ctggcgccga tctcgaaatc ccctgtcacc ccgtcctggc cccgatccgc aaccttgcct
     1861 gtcttggctc tcttggtagc gcaggctggt gtcagacgcg ctggccaagc ccggtcgcgc
     1921 cgcgatccgc gcgccgatgc ctcgacaggc gccagttctc gcaaccggcg cctgcggata
     1981 agtcataaac cacttactta gcgccggcta tgtcttggcg aacttgcccg ccgcgcggac
     2041 agagcccgtc gccacgccgg tcggcgcgcc gcctggcggc tcgtcggtga ccgccagcag
     2101 ggcggtgtag gcgcccgcgc cgcgcaggtc cttgggcagg gggatggccc gcggggcgtc
     2161 ggtgggcaac atgcccagcg atatggcctt ctgaccctcg gggatgatcc acagctccgg
     2221 ctcgcgccct tctggcatcg tcatggccac gggagtcagg atcagggtgt cggtggcgtg
     2281 atccagggtg gcgaccaggg cggcctggtt ggtgcccgga tccttgatca gggcgacctc
     2341 ggccggcgcg ggcgcggcgg gcgcgatgac cgacggagag ggcgctcgcg gcgtcagcac
     2401 cagggcggcc acgctggccg cagccagggc caccgcgccc accgagacgc cgcgccagac
     2461 ccgggcgttg ttccagagcg tgggcttgct ggccccgccg atcaggttgg cgatccgcgg
     2521 ccagaggcca gccgagggct cgacgccccg gatgtcggag gccaaggggg tgagccggtt
     2581 ttcccaccac tccacggcgc gggcaaagct gggctcggcg gcaatgcggg actgaacagc
     2641 ggcgcgctcg gctgcgtcca gcacgcccag cacatattcg ccggccaggg gacggtcttc
     2701 gtcggaaacg gggggcaagg cgtcggtcat gcttcgagac accagtatca gcgcggcgac
     2761 gacatagttt ttcagcgcca gtgttgcctc gtgcc
//
LOCUS       DAOMEO010000344         2786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3922_length_2786_cov_2.337971, whole genome shotgun sequence.
ACCESSION   DAOMEO010000344 DAOMEO010000000
VERSION     DAOMEO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_2786_cov_2.337971"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..326)
                     /locus_tag="PLH31_08650"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="PLH31_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(642..1796)
                     /locus_tag="PLH31_08655"
     CDS             complement(642..1796)
                     /locus_tag="PLH31_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR89317.1"
                     /translation="MSGLADLRLGFIPLTDCAPLIVAKARGFFAEEGLDVELSREVSW
                     ATVRDKVTVGALDGAHMLAPMALASTLGAGGAGDVVPMIAPLALNLNGSAITVSAPLA
                     AALREIDPEGMARHPITARPLARLVQQRKARGEPPLTFAVVFTYSMHAYELRYWMAQA
                     GIDPDRDIRLVVTPPPRMVEQMRLGGIDGFCVGAPWNAVAVQEGLGEVLIRASEFWPG
                     SPDKVFGVKVEWAQTYPGELRAALRALIRASAWADEPANREALVDLLARPEHIGAPPA
                     AIALALGSEIIFHAGAAGLPRREHALWFLSQMARWGQIAHARDLGAVADAVYRPDLFQ
                     SASASLGPVVGPAVTNAAGADHAALFDGLAFDPDRPLDYAAGFEISRLQP"
     gene            complement(1793..2149)
                     /locus_tag="PLH31_08660"
     CDS             complement(1793..2149)
                     /locus_tag="PLH31_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HQR89318.1"
                     /translation="MFVDRSEPGLAEQAVRAGVAAYIVDGLAPSRVRSILDVAISRFA
                     LTQQLRGDLAKAKADLESRKVVDRAKGLLMKERGLDEDGAYRLLRKLAMDRGKPIGVI
                     AADLLAFAGILRGDAS"
     gene            2171..2386
                     /locus_tag="PLH31_08665"
     CDS             2171..2386
                     /locus_tag="PLH31_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89319.1"
                     /translation="MTSLAQALQRVSVWTVGSDDNNVRRKSRDFGLIEKRRLPHLKGM
                     HADKTFGHQGGSREIGVNDKHAHELSV"
     gene            complement(2518..>2786)
                     /locus_tag="PLH31_08670"
     CDS             complement(2518..>2786)
                     /locus_tag="PLH31_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89320.1"
                     /translation="LVDAPDSKSGSERSVGSTPTARTINLFNMEDWISARSRDFLESH
                     FRMRRPAFCCPDRRGKARSTGVAPGLVLSGSVDELPDEPPRISA"
BASE COUNT          547 a          938 c          892 g          409 t
ORIGIN      
        1 aggccaatga cgttctgcca gccataggcc ttggcgagcg cgggagcgaa cagggccgcg
       61 agcgccgtgc cggaattgcc cgcgccggcg atacccaggg ccaggccctg atgctcctgc
      121 ggataccagc gtgaggccag cggcaaagcg acagcgaagg atgaaccggc cacgccgagg
      181 atcaggccca gggccaggac ctgattgtag ctgtgcaccc ccaccgccca ggtcaggacc
      241 aggccggtca gcacgatcag ctggccgatc atgccggtct tcttggggcc gatgcgatcg
      301 accagcacgc cattgaccag tctcagctgc gagaggggcg cctgcgcccg ggggtgaccg
      361 catccgagcg gtcgtctctg gcagccacga acgccgttgt ccgaaggcct gaccgcagtg
      421 aggattaaga gggccgtccc gtcaccccgt cttcgcaccc gcaacgccac cactggtggc
      481 tccgcacaat ggctcagcaa ataggcgccg ctgtcgctcg cttattgagc agccactggg
      541 agcgaggcgc gctaaaacgc ggtcagaacg acgccttgcg acgagcgatg cgacggccgc
      601 actacctcgt cgccatcatg tgcgaccgca agcccatggg ctcaaggctg caggcggctg
      661 atctcgaagc ccgcagcata gtccaatggc cggtcgggat cgaacgcgag gccatcgaac
      721 agggcggcgt gatcggcccc ggctgcattg gtcaccgctg ggccaacgac cggtcccagc
      781 gacgccgagg cgctctggaa cagatcgggc cggtagacgg cgtcggctac ggcgccgagg
      841 tcccgagcat gcgcaatctg cccccagcgc gccatttgag acaggaacca caaggcgtgc
      901 tcacggcgcg gcaagccagc cgcaccagca tggaagatga tttcacttcc cagggccaag
      961 gctatggctg cagggggcgc gccaatatgc tctggacggg ccaggagatc gaccagggcc
     1021 tcgcggttgg cgggctcgtc ggcccaggcc gacgcccgga tcagagcacg aagcgcggcc
     1081 cgcagttcgc cagggtaggt ctgcgcccac tcgaccttca cgccaaacac cttgtcgggt
     1141 gagccgggcc agaactccga agcgcggatc aggacctcgc cgagtccttc ctggacagcc
     1201 accgcgttcc agggcgcacc gacgcaaaat ccgtcgatgc cgcccagtcg catttgctcg
     1261 accatgcgtg gcggcggcgt gaccacgagg cgaatgtcgc gatctggatc gatcccggcc
     1321 tgagccatcc agtagcgcag ctcgtaggca tgcatggaat aggtaaagac gacggcgaag
     1381 gtcaggggcg gctcaccgcg ggcctttcgc tgctgaacca accgcgccag aggccgcgcg
     1441 gtgattggat gccgggccat gccttcgggg tcgatctcac ggagcgcagc ggccagcggc
     1501 gccgaaaccg tgatcgcgct gccgttcagg ttcagggcca gaggcgcgat catcggaacg
     1561 acatcgcctg ccccgcccgc gcccagggtc gaggcgagcg ccatgggggc cagcatatgc
     1621 gcgccgtcca gcgcccccac tgtcaccttg tcgcgaacgg tcgcccagga aacctcgcgg
     1681 ctgagctcga catccagccc ctcctcagcg aagaagcccc gcgccttggc cacaatcaaa
     1741 ggggcgcaat cggtgagtgg aataaagccc aggcgcagat cggccagccc gctcaagagg
     1801 cgtctcccct gagaatcccc gcaaaggcca aaagatcggc cgcaatgacg ccgataggct
     1861 tgccccgatc catggcgagc ttgcgcagca gccgataggc cccgtcctcg tcgagacccc
     1921 gctccttcat cagcagaccc ttggcgcggt ccacgacctt ccgggactcc agatcagcct
     1981 tggccttggc cagatcgcca cgcagttgct gcgtcaaggc aaagcggctt atggccacat
     2041 cgaggataga gcggacgcga ctgggggcca gtccatcgac aatgtaggcc gcaacaccgg
     2101 cccgcaccgc ctgctcggcc aagcccggtt cggaccggtc gacaaacatc accactggac
     2161 gggtattgct gtgaccagcc tcgcgcaggc tctccagcgt gtctcggtct ggactgtcgg
     2221 aagcgatgac aacaacgtcc ggcgaaaatc gcgcgacttc ggcctcatcg aaaagcgccg
     2281 cctgccgcac ctcaagggga tgcacgccga caagaccttc ggccaccagg gcggctctcg
     2341 cgagatcggg gtcaatgaca agcacgcgca tgaactttcg gtatgaccga tcgcgactgg
     2401 ctgggagcgt gagttcggcc aagaccgcgg ccaaccgtcg ccaacgcctt caaattgcgc
     2461 aaaacgagct gtaaaatcag gcattcaacg cctgcttgcg cggcaggtta gcgctgctca
     2521 agccgaaatc cgtgggggct catccgggag ctcgtcgacg cttcccgaca agaccaggcc
     2581 aggcgcgacg cctgttgagc gcgctttacc tcgccggtcc gggcagcaga aagccggcct
     2641 gcgcatgcgg aagtgacttt ccaggaagtc cctcgaccgg gcagagatcc agtcttccat
     2701 attgaaaaga ttgatggtgc gggcggtcgg ggtcgaaccg acactccttt cggaaccgga
     2761 ttttgagtcc ggcgcgtcta ccagtt
//
LOCUS       DAOMEO010000345         2785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3925_length_2785_cov_2.060440, whole genome shotgun sequence.
ACCESSION   DAOMEO010000345 DAOMEO010000000
VERSION     DAOMEO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3925_length_2785_cov_2.060440"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLH31_08675"
     CDS             complement(<1..715)
                     /locus_tag="PLH31_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89321.1"
                     /translation="MSELARLLLFVAIAGSAVTFLGSAAIWFMDEDRRIRRALRHVLK
                     GEPEVVILAHGRGRGAGFSFATGLAAVAWDSGAWCLIYRIDELMGAELLVDGQVVGRV
                     FRGENRRAVDQVAPQSVHTVTLRLVFDDPRHPDFDLDLWVPGDEQRRESRSPADAMQE
                     ANRWLARCEAIVRRPRAPRPAPVVKTAPPPEPPPPPPQRPEPPRPAPTPAPVLAAEEG
                     FEDDEDDEPSFDLDEPPWEI"
     gene            complement(757..>1339)
                     /locus_tag="PLH31_08680"
     CDS             complement(757..>1339)
                     /locus_tag="PLH31_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR89322.1"
                     /translation="LSLHPVEITARALPDAVADEPARYIEGKVATPKGPVTVGGLYLP
                     NGNPIGTEKFDYKLAWMRRLQAHAKSLLALEEPLVILGDYNVIPEAEDVHNPEAWLGD
                     ALFQPESRAAFRALKNLGFTDAYMQVDGAPGGYTFWDYQAGAWPRNLGIRIDHVLLSP
                     QAADRLVDLVIHRDERDKEKPSDHVPVVAELNL"
     assembly_gap    1340..1349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1350..1703)
                     /locus_tag="PLH31_08685"
     CDS             complement(<1350..1703)
                     /locus_tag="PLH31_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR89323.1"
                     /translation="MRIATWNVNSVNARLEGVLAWFEAQAPDVAVLQEIKCVDEKFPT
                     EAFERLGYNVAVHGQKTYNGVALLSKFPLEDVRRGLPFLHEDETDEQSRYIEAVIAAP
                     TPVRVCGLYLPNGNPI"
     assembly_gap    1707..1716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1717..2077
                     /locus_tag="PLH31_08690"
     CDS             <1717..2077
                     /locus_tag="PLH31_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89324.1"
                     /translation="GAKRLGRMTTTLKSGFKISDRGQQVLLVLITLVWSAAAVIGAIL
                     AVFSPLVFDGPGNLRNPLVWLGFVLGALFWAVCMLAPLVGWIQWRKGKRTEAWAAMAA
                     PAAWGALALTVLQFVPG"
     gene            complement(2081..2413)
                     /gene="erpA"
                     /locus_tag="PLH31_08695"
     CDS             complement(2081..2413)
                     /gene="erpA"
                     /locus_tag="PLH31_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HQR89325.1"
                     /translation="MTEATVTLSPNAARRIHAIGQTEGRPVMLRVAVEGGGCSGFQYR
                     FDLVETAEADDLKIERDGAAALVDVISMALLKGSEIDFVDELAGAEFRVRNPNAKSSC
                     GCGVSFSI"
     gene            2503..>2785
                     /locus_tag="PLH31_08700"
     CDS             2503..>2785
                     /locus_tag="PLH31_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480012.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQR89326.1"
                     /translation="MTSPYLVARAPYAEDPAKSRGRRFREEESRTRTPFARDRDRIIH
                     TSAFRRLKEKTQVFVAHEGDNFRTRLTHSLEVAQVARSLATALGLDSDLA"
BASE COUNT          464 a          903 c          941 g          457 t
ORIGIN      
        1 cgatttccca cggcggctca tcgaggtcga agctgggctc gtcatcctcg tcgtcctcga
       61 agccttcttc ggcggcaaga accggggcgg gcgtcggcgc gggccgtgga ggctcgggtc
      121 tttgcggcgg cggcggcggc ggctctggcg gcggagccgt cttcacgacc ggggccggac
      181 gcggggcccg cggtcggcga acgatcgcct cgcagcgagc cagccatcgg ttggcctcct
      241 gcatggcgtc ggccggcgag cggctctcgc ggcgctgctc gtcgccggga acccacaggt
      301 cgagatcgaa gtcgggatgg cgcggatcgt cgaacaccag gcgcagcgtg accgtatgca
      361 ccgactgcgg cgcgacctgg tcgaccgcgc gccggttctc gccccggaac acccggccca
      421 caacctggcc gtcgaccagc agttcggcgc ccatcagctc atcgatgcga tagatcagac
      481 accaggcccc gctgtcccag gcgacggcgg ccaggccggt tgcgaacgag aagcccgcgc
      541 cgcgaccccg gccatgggcc aggatcacca cctcgggctc gcccttcagc acgtggcgca
      601 gggcgcggcg gatgcgccgg tcctcatcca tgaaccagat ggccgcgctg cccaggaacg
      661 tcaccgccga gccggcgatg gcgacaaaca gcagaaggcg ggcgagttcg ctcatggggg
      721 ccaaggctag cgagagtccc ggattcgcaa cagggactag aggttcagct cggcgaccac
      781 cggaacgtgg tccgatggct tttccttgtc ccgctcgtcg cggtggatca ccaggtcgac
      841 cagtcggtcg gcggcctgtg gcgagagcag cacatggtcg atgcgaatcc ccaggtttcg
      901 cggccaggcg ccggcctgat agtcccagaa cgtatagccg cccggcgcgc cgtcgacctg
      961 catgtaggcg tctgtgaagc ccaggttctt cagggcccgg aatgcggccc ggctctcggg
     1021 ctggaacagg gcgtcgccca gccaggcctc gggattgtgg acatcctcgg cttcgggaat
     1081 gacgttgtaa tcacccagga tgacgagcgg ctcctccaga gccagcaggc tcttggcatg
     1141 ggcctgcaac cgtcgcatcc aggccagctt gtaatcaaac ttctcggtgc cgatcggatt
     1201 gccgttgggc agatagaggc cgccaaccgt gaccgggccc ttgggcgtgg caaccttgcc
     1261 ctcgatatag cgggcgggtt cgtctgcgac tgcatcaggc agcgcgcgcg cggtgatctc
     1321 gacgggatga agcgagagan nnnnnnnnng atcggattgc cgttgggcag atagaggccg
     1381 cagaccctca ccggcgtggg cgcggcgatc acggcctcga tatagcgcga ctgctcgtcg
     1441 gtctcgtctt cgtgcaggaa gggcaggccg cgccggacgt cctccagcgg gaacttcgac
     1501 agcagggcga cgccattata ggtcttctgg ccgtgaacgg cgacattata gccaagcctt
     1561 tcaaaggctt cggtcgggaa tttctcgtcg acgcacttga tttcctgcag aacggcgacg
     1621 tccggggcct gcgcctcgaa ccaggccagc accccttcca ggcgagcatt gacggaatta
     1681 acgttccagg tggcgatacg cattgannnn nnnnnngggc gcgaagaggc tagggcgcat
     1741 gacgaccacg ctcaagtcgg gcttcaagat ttcggacagg ggacagcagg tgctgctggt
     1801 cctgatcacg ctcgtctggt cggcggcggc ggtgatcggc gccatcctgg cggtgttttc
     1861 gccgcttgtc ttcgacgggc cgggcaatct caggaatccc ttggtctggc tgggcttcgt
     1921 gctcggggcg ctgttctggg cggtctgcat gctggcgccg ctggtgggct ggatccaatg
     1981 gcgcaagggc aagcgcaccg aggcctgggc ggccatggcc gcaccagccg cctggggcgc
     2041 cctggccttg accgtcctgc agttcgtgcc gggctaaagc ctagatcgaa aagctgacgc
     2101 cgcagccgca gctggacttg gcgttgggat tgcgcacccg aaattccgcg ccggccagtt
     2161 cgtcgacgaa atcgatctcc gagcccttca gcagggccat agagatcacg tccaccaggg
     2221 cggcagcccc atcgcgctcg atcttcagat cgtcggcctc ggcggtttcc accagatcaa
     2281 agcggtactg gaagcccgag cagccgccgc cctccaccgc gacgcgcagc atgacggggc
     2341 gcccctcggt ctggccgatg gcgtgaatgc ggcgcgcggc gttgggagaa agggttacgg
     2401 ttgcttcggt catgactgtg gaaaactcat ccttgaccta tatgaggata tagacgcccc
     2461 aacggaaggg gcgaaagccc agaagcggtc caagagcggt tcatgacgtc tccctacctt
     2521 gttgcgcgcg ccccctatgc cgaggatcca gccaagtcgc gtgggcgccg gttcagggag
     2581 gaggagagcc gcacccgcac gcccttcgcc cgcgatcgcg accggatcat ccacacctcg
     2641 gcctttcgcc ggctgaagga aaagacccag gtcttcgtgg cccatgaagg cgacaacttc
     2701 cgcacccgcc tgacccattc gctggaagtg gcccaggtgg cgcgctcgct ggccacggcc
     2761 ctgggactgg actccgatct ggccg
//
LOCUS       DAOMEO010000346         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3937_length_2777_cov_2.036003, whole genome shotgun sequence.
ACCESSION   DAOMEO010000346 DAOMEO010000000
VERSION     DAOMEO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_2777_cov_2.036003"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLH31_08705"
     CDS             <1..553
                     /locus_tag="PLH31_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR89327.1"
                     /translation="LVMALGPAGTGKTYIAIAKAVEALEAGSVARIVLSRPAVEAGES
                     IGYLPGDMEDKLAPYLRPLYDALTDRLSSRRMRALMADGSIEIAPVGFMRGRTLNNAF
                     VVIDEAQNCTYMQLKMLLTRLGWNSTMVVTGDPNQSDLLPGISGLADVAAKFDALENI
                     GVVRLADRDIVRHPLVAEMLGVL"
     gene            715..1083
                     /locus_tag="PLH31_08710"
     CDS             715..1083
                     /locus_tag="PLH31_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89328.1"
                     /translation="MLVKTILIAAAGLSCASLLATAAAAQTSTPAPTPQPQPQPQQQP
                     MPQNHFAPAWMHPQEQIWRQSLTDTAQTAPTKAQWARARRAADLVNANRCDEAFSLAR
                     TEKDTRLETGVSKVCLARKS"
     gene            1113..1412
                     /locus_tag="PLH31_08715"
     CDS             1113..1412
                     /locus_tag="PLH31_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89329.1"
                     /translation="MKKIVLFLAAGATLMATGPAFAQDPASYPRHSMGSPPSLDPAFR
                     PSRDVRHREHRARIAAGLIRNGRCDAAARLAMRQGDKPMLSRISEVCRTEKASGA"
     gene            1519..>2777
                     /locus_tag="PLH31_08720"
     CDS             1519..>2777
                     /locus_tag="PLH31_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQR89330.1"
                     /translation="MADAAAAVPRSEFTARGVLLGIAITLVFTAAQVYLGLKVGLTFA
                     TAIPAAVISMALLRAFKTSTIQENNIVQTIASAAGTLAAVIFVLPGLLMIGWWAHVPF
                     FTTFMACAVGGILGVMYTIPLRRALVTNSNLPYPEGVAAAEVLKVGTGSREGAAEGKA
                     GLIAVAAGTLASALFAALAAAKIFAGEVAGYFKAGAGATGLGASSSLALMGAGHLMGI
                     TVGIAMFAGLVIAWAVLVPIMTQVFPMPGASAADHALTVWSTKVRFLGAGVIGAAAIW
                     TLGKLIAPIWQGLVSAVEASKARKVDGAVLPRVEQDIPIVIVGIVSTALLAPAGWVMA
                     HFLSGGPLSAVATPLIIAAVAFLVFAGLLAAAVCGYMAGLIGSSNSPVSGIAILTVLG
                     ASLMVGAIGRTTIGPDVATALVAFALFV"
BASE COUNT          424 a         1003 c          880 g          470 t
ORIGIN      
        1 cctggtgatg gccctgggcc cggcaggcac cggcaagacc tacatcgcca tcgccaaggc
       61 cgtcgaagcc ctggaagccg gaagcgtggc ccgcatcgtg ctgtcgcgcc ccgctgtcga
      121 ggccggcgaa tcgatcggct accttcccgg cgacatggaa gacaagctgg ccccctatct
      181 gcgcccgctc tacgatgccc tgaccgaccg gctctccagc cgccgcatgc gcgccctgat
      241 ggctgatggc tccatcgaga tcgcacccgt cggcttcatg cgcggccgca ccctgaacaa
      301 cgccttcgtg gtgatcgacg aagcccagaa ctgcacctac atgcagctga agatgctcct
      361 gacgcgcctg ggctggaatt ccacgatggt ggtcacgggc gatcccaacc agtccgacct
      421 gctgccgggc atttcgggcc tggccgacgt ggccgccaag ttcgacgcgc tcgagaacat
      481 cggcgtggtc cgcctggccg accgtgacat cgtccgccac ccactggtcg cggaaatgct
      541 gggcgtgctc tagcgcgacg agccgcttcg cccgacgacg gggcgaggcg gctcatttcg
      601 attaaatcgc tttacggcaa gcggaggttg tgcggtaatt gccacagagc ctccgcgtta
      661 gtgttgcggg ctcctcgttc agccggcgct cagcgcgccc agagaggaag acacatgctc
      721 gtcaagacca tcctgatcgc ggccgccggc ctgtcctgcg ccagcctgtt ggccacagcc
      781 gccgcagcgc aaaccagcac cccggcgccg accccgcagc cgcagccgca gccgcaacaa
      841 cagcccatgc cccagaacca tttcgcgccg gcatggatgc acccgcagga gcagatctgg
      901 cgtcagtcgc tgaccgacac cgcccagacg gcgccaacca aagcccagtg ggcgcgcgcc
      961 cgtcgcgccg ccgatctggt aaacgccaat cgttgcgatg aagccttcag cctggcgcga
     1021 accgaaaaag acacccgcct cgaaaccggc gtcagcaagg tctgcctcgc gcgcaaaagc
     1081 tgaaagctct gaactcagcc aaggagacgt cgatgaagaa gatcgttctt ttcctcgcgg
     1141 ccggagctac cctcatggcg acgggtcccg cgttcgcgca ggacccggct agctatcctc
     1201 gccactcgat gggcagcccg ccttccctgg acccggcctt caggccgtcc agggacgtta
     1261 ggcaccggga acatcgggcg cgcatcgccg ccggcctgat ccgcaatggt cgctgcgatg
     1321 cggccgcgcg cctggccatg cgtcagggcg acaagcccat gctctcgcgg atcagcgagg
     1381 tctgtcggac cgagaaagcc tcgggcgcat agagctagag ctacaggcgc tggatttccg
     1441 aagtcgaaaa tccagtcttg agtttgaggc ggtcggacgt gcaaggtctg gccgcctcaa
     1501 ctgtttcagg accccttcat ggctgacgcc gccgccgccg ttccgcgttc ggaattcacc
     1561 gctcggggcg ttctgctcgg gattgccatc accctcgtct tcacggcggc acaggtctat
     1621 ctgggcctca aggttggcct gaccttcgcc accgcgatcc cggcggctgt aatctcgatg
     1681 gccctgctta gggccttcaa gacctcgacc atccaggaaa acaacatcgt ccagaccatc
     1741 gcgtcggcgg cgggcaccct ggcggcggtg atcttcgtcc tgccgggcct gttgatgatc
     1801 ggctggtggg cgcacgtgcc gttcttcacc accttcatgg cctgcgcggt cggcggtatt
     1861 ctgggggtga tgtacaccat ccccctgcgc cgggccctgg tgaccaattc caacctgccc
     1921 tatccagagg gcgtcgccgc cgccgaggtg ctgaaggtcg gcaccggctc gcgcgagggc
     1981 gccgccgagg gcaaggccgg cctgatcgcg gtcgccgccg gcaccctggc ctcggccctg
     2041 ttcgccgcat tggcggccgc caagatcttc gctggcgaag tggctggtta cttcaaggcc
     2101 ggcgccggcg cgaccggtct gggcgcctcc agctcgctgg ccctgatggg cgcgggccat
     2161 ctgatgggca ttaccgtcgg catcgccatg ttcgcgggcc tcgtcatcgc ctgggccgtt
     2221 ctggtgccga tcatgaccca ggtcttcccg atgcccggcg ccagcgccgc cgaccacgcc
     2281 ctgacggtgt ggagcaccaa ggtccgcttc ctgggggccg gcgtcatcgg cgccgccgcc
     2341 atctggaccc tgggcaagct gatcgcgccg atctggcagg gtctggtctc ggccgttgag
     2401 gcgtcaaagg cccgcaaggt cgacggcgcg gtcctgccgc gcgttgagca ggacatcccc
     2461 atcgtcatcg tcggcatcgt gtccaccgcc ctgctggccc ccgccggctg ggtgatggcc
     2521 cacttcctga gcggcgggcc gctgtcggcc gtcgccacgc cgctgatcat cgccgccgtg
     2581 gccttcctgg tgttcgccgg cctgctggcc gccgccgtct gcggctacat ggccgggctg
     2641 atcggctcgt ccaacagccc cgtctcgggc atcgccatcc tgaccgtact gggcgcctcg
     2701 ctgatggtcg gcgcgatcgg ccgcacgacg atcggaccgg acgtcgccac cgccctggtc
     2761 gccttcgccc tgttcgt
//
LOCUS       DAOMEO010000347         2771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3949_length_2771_cov_2.387334, whole genome shotgun sequence.
ACCESSION   DAOMEO010000347 DAOMEO010000000
VERSION     DAOMEO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_2771_cov_2.387334"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..574)
                     /locus_tag="PLH31_08725"
     CDS             complement(104..574)
                     /locus_tag="PLH31_08725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQR89331.1"
                     /translation="MTWATDRLDALKAGTATPPPIVETLKLGLIDDWGEGWVRKSWSP
                     SADLATADGSLFGGYLAALADQVLAFAAMTVVPHDRFYRTVNLQVNFLKVGRSHPLVI
                     EARIVAQTRQLITARAEFRRDDGALIADATAQQILMAFDHWPAEQAAIEALGGR"
     gene            671..1273
                     /locus_tag="PLH31_08730"
     CDS             671..1273
                     /locus_tag="PLH31_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89332.1"
                     /translation="MSAIETVRKIPRQRRARASVDFVLEAAAQVLEAEGEAGFNTNAV
                     AGRAGVSIGTLYRYFPDKQAILLALALRETEAHRQAITLVLEGAERGLAKDRAMIRAF
                     LGAFAGRSRARRIAMTTLLAQADHSQLAARFAAAESGLTDAQGRPLTRVQAFVLARSV
                     QGAMRAAVLEGADFLLSQEFEDELVRLSRAYLGYPTPRLA"
     gene            complement(1289..2152)
                     /gene="mutM"
                     /locus_tag="PLH31_08735"
     CDS             complement(1289..2152)
                     /gene="mutM"
                     /locus_tag="PLH31_08735"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HQR89333.1"
                     /translation="MPELPEVETVRRGLEPVLSGARLSRVRANRPDLRFPLPEGFVQR
                     LTGAKILRLDRRAKYLLAPLDRGDTLVMHLGMTGRFEIAAPEGTIRPGAFAREVKPDD
                     KHAHVVFETEAGAVVTYHDPRRFGFMDLIATDRVDRHPWFATMGPEPLGEGFNPQTLI
                     KAFAGRKQGPKTLLLDQKTVAGLGNIYVCEALHRAGISPFKPSGMIAGKRLGPLTTII
                     KEVLAEAVEVGGSTLKDFAAADGALGYFQHRFRVYDREGEPCPTSGCGGVIAREVQAG
                     RSTFFCPVCQV"
     gene            2234..>2771
                     /locus_tag="PLH31_08740"
     CDS             2234..>2771
                     /locus_tag="PLH31_08740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HQR89334.1"
                     /translation="MSKPAATFGFKDVDPSVKAGLVRGVFDRVAKNYDLMNDLMSGGV
                     HRLWKDAVAARLNPQPGEVIIDCAGGTGDMARRFAKMARRAQERRGGPDAMINIIDYN
                     AEMIAAGIEKGGEPEITWTVGDAQRLPLPDAYADAYVISFGIRNVTDIDAALREARRV
                     LKPGGRFLCLEFSRPVTEA"
BASE COUNT          481 a          909 c          940 g          441 t
ORIGIN      
        1 ccggcacgct gcagcctcca agagatttaa ggatgagcgg gacgcggggc gtccggtttc
       61 ttggagagcg aggccaagac ttcgacatcg ctcaaacaga catctaccgc ccacccaaag
      121 cctcgatggc cgcctgctcc gccggccaat ggtcgaaggc catcaggatc tgctgggcgg
      181 tggcgtcggc aatcaaagcc ccatcgtccc gacgaaactc ggcgcgtgcg gtaatcagct
      241 gccgagtctg ggccacaatg cgcgcctcga tgacgagggg gtggctgcgc cccaccttca
      301 ggaagttcac ctgcagattt accgtccggt agaaccgatc gtgcggaacc accgtcatgg
      361 ccgcaaaggc caggacctgg tcagccagcg ccgccagata gccgccgaac agcgagccgt
      421 cggcggtggc gagatccgcg ctcggactcc aagacttgcg cacccagcct tcgccccagt
      481 cgtcgatcag gcccagcttc agggtctcaa cgatcggcgg cggggttgcg gtccccgctt
      541 tcaaggcgtc gagtcgatcg gtcgcccacg tcatcctgtt ctcccgccaa gttgcgtggc
      601 gaagggtagg gttggtgggc ggcctctgtt actcgactag tcaagaagac ggcgtcgtgg
      661 ggggccttag gtgagcgcta ttgaaaccgt tcgcaaaatt ccccggcaac ggcgcgcccg
      721 agcctccgtt gacttcgttc tggaggctgc agctcaggtt ctggaggccg aaggggaagc
      781 cggattcaac accaatgcgg tggctgggcg cgcgggggtg agcatcggca cgctctatcg
      841 ctactttccg gacaagcagg cgatcctgct ggccctggct ctacgagaga cagaagcgca
      901 ccggcaggcg atcacgttgg tgctggaggg agccgaacgg ggtctggcca aggacagggc
      961 gatgatacgc gcgtttcttg gagccttcgc tggtcgcagt cgggcgcgac ggatcgccat
     1021 gacaaccctg ctggcgcagg cagatcatag ccaactggcc gcaaggttcg cggcggcgga
     1081 aagcggcctg accgacgcgc agggccgtcc tctgacgcgg gttcaagcct ttgttctggc
     1141 tcggtccgtg caaggggcca tgcgggccgc cgtcctggaa ggcgccgact tcctgttgag
     1201 ccaggagttc gaggatgagc tggtgcggct cagccgggcc tatctgggct atccgacacc
     1261 aaggctcgcc tgaactcgaa acgctccctc acacctgaca caccggacaa aagaacgtcg
     1321 accgcccggc ctgaacctcc cgcgcgatca cgcccccaca ccccgacgtc ggacacggct
     1381 ccccctcgcg gtcgtaaacc cggaaccggt gctggaaata gcccagcgcc ccgtcggcgg
     1441 cggcgaagtc cttgagggtg gagccgccca cctcgacggc ctcggccagg acctccttga
     1501 tgatcgtggt cagcggtccc agccgcttgc cggcgatcat ccccgagggc ttgaatggcg
     1561 agatgcccgc ccggtgcagg gcctcgcaca catagatatt gcccaggccc gcgacggtct
     1621 tctggtcgag cagcagggtc ttggggcctt gcttgcgacc cgcgaaggcc ttgatcaggg
     1681 tctgggggtt gaacccctcg cccagtggct cgggacccat ggtcgcgaac cacggatggc
     1741 ggtcgacgcg gtcggtggcg atcaggtcca tgaacccgaa gcggcggggg tcgtgatagg
     1801 tgaccaccgc gcccgcttcg gtctcgaaga ccacgtgggc gtgcttgtcg tcgggcttga
     1861 cctcgcgggc gaaggcgccg gggcgtatcg tgccttccgg ggcggcgatc tcgaagcgcc
     1921 cggtcatgcc cagatgcatc accagggtgt cgccacgatc cagcggggcc agcagatatt
     1981 tggcgcggcg atccagccgc aggatcttcg cccccgtcag ccgctggacg aaaccctcgg
     2041 gtagcgggaa ccgcaggtcg ggccggttgg cgcgcacgcg gctcaggcgg gcgccgctca
     2101 ggacgggctc caggccgcgc cggacggtct cgacttcggg caattcaggc aagggcggcc
     2161 tcaaggtgat gacgcgacgc ggtcggcggt ctaagagcga tccagcatat agagagcctg
     2221 gtcgatagac gtcatgagca agcctgccgc cacattcggt ttcaaggacg tcgatccttc
     2281 ggtgaaggcc ggcctggtgc gaggcgtctt cgatagagtc gccaagaact acgacctgat
     2341 gaacgacctg atgagcgggg gcgtccaccg gctgtggaag gacgccgtcg ccgcgcgcct
     2401 gaacccgcag ccgggcgagg tgatcatcga ctgcgccggc ggcaccggcg acatggcccg
     2461 ccgcttcgcg aagatggccc gccgcgccca ggagcgccgg ggcggccccg acgcgatgat
     2521 caacatcatc gactacaatg ccgagatgat cgccgccggc atcgagaagg gcggcgagcc
     2581 ggaaatcacc tggaccgtgg gcgacgccca gcgcctgccg ctgcccgacg cctatgccga
     2641 cgcctatgtg atctcgttcg gcattcgcaa tgtcaccgac atcgacgcgg ccctgcgcga
     2701 ggcgcggcgg gtgcttaaac ccggcggccg cttcctctgc ctggagtttt cgcggccggt
     2761 caccgaggcg c
//
LOCUS       DAOMEO010000348         2769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3953_length_2769_cov_2.279293, whole genome shotgun sequence.
ACCESSION   DAOMEO010000348 DAOMEO010000000
VERSION     DAOMEO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_2769_cov_2.279293"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /gene="rplI"
                     /locus_tag="PLH31_08745"
     CDS             <1..573
                     /gene="rplI"
                     /locus_tag="PLH31_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQR89335.1"
                     /translation="LLERVEGTGTLGDVVTVKDGFARNFLLPRHKALRANAANMKSFE
                     VQRAEIEARNVKNREAAGANGETLDGTQYVMIRQAGESGQLYGSVAGRDVADAIKAEG
                     GKVDRSMIVLDKPIKTLGVHEVKVRLHAEVTVTVTLNIARSADEAERQSRGEDVVAAQ
                     FEEDRAADAEAASDMAAGGAGSFEGDHYES"
     gene            770..1336
                     /locus_tag="PLH31_08750"
                     /pseudo
     CDS             770..1336
                     /locus_tag="PLH31_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517111.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     assembly_gap    1337..1346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1347..1941
                     /locus_tag="PLH31_08755"
                     /pseudo
     CDS             1347..1941
                     /locus_tag="PLH31_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517111.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     assembly_gap    1942..1951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1952..>2769
                     /locus_tag="PLH31_08760"
     CDS             <1952..>2769
                     /locus_tag="PLH31_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89336.1"
                     /translation="SIRAGEPTSYNRGPLTSAAAGAQGFPGFQPSNVLSEDRSNIGVY
                     LDLESRLTERFTASAAVRYEDYSDFGATTTGKLAARFELADGFAVRGAASTGFRAPAL
                     QQQFFTTTSTNFIIINGVSTPVEVGTFPATSAVAKVLGAQPLEAEESVNYSLGFVFHR
                     GPFEVTVDAYRINIDNRIVLSENIQGSATGTPTLVTINNLLQPFNVTAARFFINGVET
                     ETQGLDVVARYRLDADEGGVFDLTAAANFNETDVTRVPTTNTLSSLPTPPTLFG"
BASE COUNT          502 a          988 c          815 g          444 t
ORIGIN      
        1 ctgctcgaac gcgttgaagg cactggcacc ctcggcgatg tcgtcaccgt gaaggacggc
       61 ttcgcccgta acttcctgct gccgcgtcac aaggccctgc gcgccaacgc cgccaacatg
      121 aagtccttcg aagttcagcg cgctgaaatc gaagcccgta acgtcaagaa ccgcgaagcc
      181 gcgggcgcca acggcgaaac cctggacggt acgcaatacg tgatgatccg tcaggctggc
      241 gaaagcggtc agctgtacgg ttcggtcgca ggtcgcgacg tcgccgacgc catcaaggcc
      301 gaaggcggca aggtcgatcg ctcgatgatc gtgctggaca agccgatcaa gacgctgggc
      361 gttcacgaag tgaaggtccg tctgcacgcc gaggtgaccg tcacggttac cctcaacatc
      421 gcgcgcagcg ccgacgaagc cgagcgccag tcgcgcggcg aagacgtcgt cgccgctcaa
      481 ttcgaagaag accgcgcggc tgacgccgaa gcggcctcgg acatggccgc cggtggcgcc
      541 ggttcgttcg aaggcgacca ctacgagtcc tgatcctttc ggatcaaagc actctcggga
      601 aacggcgcgg agcaatccgc gccgtttttt tctgcgtttc cgcctagaaa tgttcaactt
      661 gtaggcgccg cccgacgcgc ccatgttctc tcttgcgagg cgcgaagcct ccgaacccgg
      721 acagaccgat ctcaaatcgg cccccgggga aacaatgaga ggaatgaaca tgagctttcg
      781 aatcctgctg ttcactgcag catccgccgc tgcgatcgcc agctccgccc acgcccagtc
      841 cacaccgccc gccgacgccg ccaaccttga agaagtggtt gtcaccggca cgcgtacggc
      901 gggtcgcacg cgcctcgaca ccatcgcgcc tgtcgatgtg gtcaacgaaa aggccctgac
      961 ccgcaccggt acgaccgaac tggcccagtc gctgtcgacc ctggcgccct cgatcaactt
     1021 cccgcggccc gccatcaccg acggcaccga ccatatccgt cccgccaccc tgcgcggtct
     1081 atcccccgac cagaccctgg tgctggtcaa cggaacccgt cgccacgcca cggccctggt
     1141 caatgtcaac ggatccatcg gccgcggctc ggctgccgtc gacctgaacg ccatcccgac
     1201 catcgcactc aatcgcgtgg aagtgctgcg tgacggggcc tcggctcagt atggctccga
     1261 cgccatcgct ggcgtcatca acctgcgcct gcgtgaagcc cgcagcggcg gcggcgccgt
     1321 ggccccctat ggccgcnnnn nnnnnngtga ccctgcaagc ctggcagggc ttgcccctgg
     1381 gccccgaagg cttcctgacc atttcggccg agtaccgcga ccgcaacccg accagccgcg
     1441 gcgacatcga cgttcgtgtc acgccggccc gggtaacctc gcgctatggc gacgccgaag
     1501 tcaccgacaa gacgatctac ttcaacgccg gaacgccgat cagcgaaacc tgggatgcgt
     1561 acggctgggc aggctatcag gcccgtgaag gctccagcgc cgcattcccg cggattgctg
     1621 gcgacgggcg ccagacgccc gtggcctcga tctatcccaa cggatatctg cccctcatcg
     1681 ccaccgacat cgtcgacacc acggcggcgg cgggggtgcg cggcgaactg ggcggcttca
     1741 acatcgacct gaacgtcgtc tacggtgaga acgagatcga ttatcgcacc gagaactccg
     1801 ccaacgcctc gtacggcgcg gccagccaga ccagcttcaa ctcgggcggc atgagctatg
     1861 accagaccgt gattggcctg gacgtctcgc ggggtgtcga catcggcgcc tatgagccgc
     1921 tgaacgtcgc cttcggcgcc gnnnnnnnnn nattcgatcc gcgcgggtga gccaacctcc
     1981 tacaatcggg ggccgctcac gtcggcggcg gccggcgcgc aaggcttccc gggcttccag
     2041 ccgtccaacg tgctgtccga ggatcgcagc aatatcggcg tctatctcga cctggaatca
     2101 cgcctgaccg agcgcttcac cgcttcggcg gcggtacgtt acgaggacta ttccgacttc
     2161 ggcgcgacca ccacgggcaa gctggccgcg cgctttgaac tggccgatgg cttcgccgtg
     2221 cgcggcgccg cctcgaccgg cttccgggcc ccggccctgc agcagcagtt cttcacgacc
     2281 acttcgacca acttcatcat catcaacggc gtctcaaccc cggtcgaggt cggcaccttc
     2341 cccgccacca gcgccgtcgc caaggtgctg ggcgcccagc cgcttgaggc ggaggagtcg
     2401 gtcaactact cgctcggctt cgtcttccat cgcggaccgt tcgaggtgac ggtcgacgcc
     2461 tatcggatca acatcgacaa ccgcatcgtg ctgtcggaga atatccaggg ctcggccacg
     2521 ggtacgccga ccctggtgac gatcaataac ctgctgcagc ccttcaatgt cacagccgcc
     2581 cggttcttca tcaacggcgt cgagaccgaa acacagggcc tggatgtcgt cgctcgctac
     2641 cgcctcgacg cggacgaagg cggcgtcttc gacctgacgg ccgcggccaa cttcaacgag
     2701 accgatgtca cccgggttcc aacgaccaat accctgtcgt cgctccccac cccgcccacg
     2761 ctgtttggg
//
LOCUS       DAOMEO010000349         2751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3980_length_2751_cov_2.441395, whole genome shotgun sequence.
ACCESSION   DAOMEO010000349 DAOMEO010000000
VERSION     DAOMEO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3980_length_2751_cov_2.441395"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..388)
                     /locus_tag="PLH31_08765"
     CDS             complement(185..388)
                     /locus_tag="PLH31_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR89337.1"
                     /translation="MTVDRMVLAFAGFVVLLGIGLAHYVHPWWIGLSIFAGLNMIQAA
                     FTGFCPAAMIFRKLGVRPGAAFR"
     gene            complement(468..818)
                     /locus_tag="PLH31_08770"
     CDS             complement(468..818)
                     /locus_tag="PLH31_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQR89338.1"
                     /translation="MRQLVCPACGATNRAPEDRNALAAKCGRCGERLFSGVPTEVSGA
                     QLAAHSGKSRGVAVLVDVWAPWCGPCRSMAPQFAAAAGRLEPDVRLLGRWASRAFRLC
                     CSIGTAPSLRGPPG"
     gene            complement(836..2116)
                     /locus_tag="PLH31_08775"
     CDS             complement(836..2116)
                     /locus_tag="PLH31_08775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQR89339.1"
                     /translation="MAKPRIVILGAGLGGAIAAFEIKQAVGDKAEVEVVSQGDTFHFV
                     PSNPWVAVHWRKREAIEVKLPPVFKKRGIAFRGIGAAKVMPERNQLELTDGVLLDYDY
                     LVIATGPELAFDEIPGLGPHGGFTQSICHVDHAETAATAFDRFVENPGPIVVGAVQGA
                     SCFGPAYEFALILDTELRRRKVRDRVPMTFVTSEPYVGHLGLDGVGDTKGLLESEMRQ
                     RHIKWITSAKVASVEAGVMHVEEIADDGAVKATHDLPFSYSMMLPAFRGVAAVRGIEG
                     LTNPRGFILVDKHQRNPTYQNVMALGVCVAIAPTGKTPVPVGVPKTGFMIESMVTAIA
                     HNFKEILDGKPAIHEATWNAICLADFGDGGVAFVAQPQIPPRNVNWSASGKWVHLAKV
                     GFEKYFLSKVRRGESEPFYEKLALDIMGIHKIKP"
     gene            2270..2614
                     /locus_tag="PLH31_08780"
     CDS             2270..2614
                     /locus_tag="PLH31_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR89340.1"
                     /translation="MNFEPHALKTSADEACALLKALANPHRLMIVCSLIDGEQSVGAL
                     AQRLEIRETLASQHLGLLRRDGVVAARRDGQTIYYGLRSGQARAMVETLSRLFCAATE
                     TAPCPAPFKETL"
     gene            2614..>2751
                     /locus_tag="PLH31_08785"
     CDS             2614..>2751
                     /locus_tag="PLH31_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR89341.1"
                     /translation="MFGSPKTKDLTAHQVKTALDAQEILLVDVREPAEFAAERIAGAV
                     NI"
BASE COUNT          486 a          921 c          899 g          445 t
ORIGIN      
        1 aggcggccgg cgcgggcctg ggccaggcgc gccgaggccg catccgactg ggcccgggcc
       61 tcggccagac cgggattggc gcgccgcgcc agggcgatgg cctcgggcaa ggtcatggcc
      121 gaagcgccca ggggcgcggc ggcgctgacc agcagggcca gggcgaggac ggcgcgcaac
      181 gggctcaccg gaacgcagcg cccggcctga cgccgagctt cctgaagatc atcgcggcgg
      241 gacagaaccc ggtaaacgcc gcctggatca tgttgaggcc cgcgaaaatc gacaggccga
      301 tccaccaggg atggacatag tgggccaggc caatgcccag cagaacgaca aatccggcaa
      361 aggccagaac catacggtcg acagtcatga tcaacctcct ttaggagccg aggcgttcgc
      421 ctgggccagg gcctggtgcg tccaggcaac gatctgggcc gtgctcatca acccggcgga
      481 ccgcgcaatg atggcgccgt cccgatagag cagcagagcc ggaatgcccg agacgcccag
      541 cgccccagca gcctgacgtc cggttcgagc cgtcccgccg cggccgcaaa ctgcggggcc
      601 atggaccggc agggtccgca ccaaggcgcc cacacatcga ccagcacggc gacgccccgg
      661 ctcttgccgg agtgagcggc cagttgcgcg ccgctcacct cggtcggaac gccggaaaac
      721 aggcgctcgc cgcagcggcc gcacttggcc gccagggcgt ttctgtcctc tggcgcgcga
      781 ttggtcgcgc cgcaggcggg gcaaacaagc tgccgcatca caatgccttt cgccctcagg
      841 gcttgatctt gtggatgccc atgatgtcga gggccagctt ctcgtagaac ggttcgctct
      901 cgccgcgccg gaccttcgag aggaaatatt tctcgaaccc gaccttggcc agatgcaccc
      961 acttgccgct ggccgaccag ttgacgttgc ggggcgggat ctggggctgg gcgacaaagg
     1021 ccacgccgcc gtcgccgaag tcggccaggc agatggcgtt ccaggtcgcc tcgtggatgg
     1081 ccggcttgcc gtccaggatc tctttgaaat tgtgggcgat ggctgtgacc atgctctcga
     1141 tcatgaagcc ggtcttgggc acacccaccg gcaccggagt cttgccggtc ggcgcgatgg
     1201 cgacgcagac ccccagggcc atgacgttct ggtaggtcgg attgcgctgg tgcttgtcga
     1261 ccaggatgaa gccgcgcgga ttggtcagac cctcgatccc gcgcacggcg gcgacgcccc
     1321 ggaaggccgg cagcatcatg gaataggaga acggcaggtc gtgtgtcgcc ttgacggcgc
     1381 cgtcgtcggc gatctcctcg acgtgcatca ccccggcttc gaccgaggcc accttggcgc
     1441 tggtgatcca cttgatgtga cgctggcgca tctcgctttc cagcaggccc ttggtgtcgc
     1501 ccaccccgtc cagacccaga tggccgacat agggctcgga ggtcacgaag gtcatcggca
     1561 cgcggtcgcg caccttgcgg cgacgcagct cggtgtcgag gatcagggcg aattcgtagg
     1621 cgggcccgaa gcacgaagcg ccctgcacgg ccccgacgac gatgggaccg ggattttcga
     1681 caaaacggtc gaaggcggtc gccgcagtct ccgcgtggtc gacgtggcag atcgattggg
     1741 tgaagccgcc atgcggaccc aggcccggga tctcgtcaaa ggccagttca ggccccgtgg
     1801 cgatcaccag ataatcgtag tcgagcagca cgccatcggt cagttcaagc tggttgcgct
     1861 cgggcatgac cttggccgcg cctatgcccc ggaatgcgat tccccgcttc ttgaacaccg
     1921 ggggaagctt gacctcgata gcctcgcgct tgcgccagtg gacagcgacc caggggtttg
     1981 acggtacgaa gtgaaaggtg tcgccctgcg acaccacctc gacctcggcc ttgtcgccaa
     2041 cggcctgctt gatttcgaag gcggcgatgg cgccgcccag tcctgcgccc agaatgacga
     2101 tgcgcggctt ggccatgatc tcgctcctta gacgccgtgg ctaggagccc aggatggctc
     2161 attccatgcg gccgactttg atgcggatca gatttgtccc gacgccaggg gctagggttc
     2221 accgcatcga cgcttgacta aatattccaa atcacgcata tacgcaacta tgaattttga
     2281 gccccacgct ctgaagacct cggcggacga agcctgcgcc ctgctcaagg cgctggccaa
     2341 tccgcatcgg ctgatgatcg tctgctcgct gatcgacggc gaacagtcgg tgggcgcgct
     2401 cgcccagcga ctcgaaattc gcgagaccct ggcctcgcag caccttggcc ttctgcgtcg
     2461 cgacggcgtg gtcgccgcca ggcgcgacgg ccagaccatc tattacggcc ttcgcagcgg
     2521 tcaggcccgc gccatggtcg agaccctgtc ccggctgttc tgcgcagcca ccgaaaccgc
     2581 cccctgcccg gcccctttca aggagacgct ttgatgttcg gatcgcccaa gaccaaggac
     2641 ctcaccgccc atcaggtgaa gaccgcgctc gacgcccagg agatcctgtt ggtcgatgtg
     2701 cgtgagcccg ccgaattcgc cgccgagcgc attgctggcg cggtcaatat t
//
LOCUS       DAOMEO010000350         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3984_length_2743_cov_2.287946, whole genome shotgun sequence.
ACCESSION   DAOMEO010000350 DAOMEO010000000
VERSION     DAOMEO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_2743_cov_2.287946"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /gene="proB"
                     /locus_tag="PLH31_08790"
     CDS             complement(<1..699)
                     /gene="proB"
                     /locus_tag="PLH31_08790"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQR89342.1"
                     /translation="MSQNPGPLASARRIVFKVGSALLVDANTGAANHAWLRAFCDDAA
                     RLRARGQQVVVVSSGAVALGRRRLGLTGRKSLTLPEKQAAAAAGQSLLMRAWEEAFEP
                     HGIGAAQILMTRDDTETRRRWLNARATVETLLGLGVVPVVNENDTVVTEEIRYGDNDR
                     LAARVAQLAGADLLVLLSDIDGLYTVDPRRDPNASHLATIAEITPQIEAMAGGANAAS
                     GVGTGGMATKIAAAR"
     gene            complement(696..1760)
                     /gene="obgE"
                     /locus_tag="PLH31_08795"
     CDS             complement(696..1760)
                     /gene="obgE"
                     /locus_tag="PLH31_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQR89343.1"
                     /translation="MKFLDQCKIFIRSGNGGGGAVSFRREKYIEYGGPDGGDGGRGGD
                     IWIEAVDGLNTLIDYRYQQHFKAETGIHGMGRGRHGAAGADITLKVPVGTQVLEEDKE
                     TLIADLDTAGMAVRLCKGGNGGWGNLHFKGPVNQAPKFANPGQEGEERWVWLRLKLIA
                     DVGLVGLPNAGKSTFLAAATAAKPKIADYPFTTLTPNLGVVDLSTSERFVLADIPGLI
                     EGASEGAGLGTRFLGHVERSAVLIHLVDATQDDIGAAWTTIRGELEAYGDELGDKSEI
                     LALNKIDALDEDTIAEKAAELEAVAGVKPRLVSGVSGKGVKELLRAAYREVRIRRGDE
                     IEEIDEEEDHVPETPGGWTP"
     gene            1942..>2743
                     /locus_tag="PLH31_08800"
     CDS             1942..>2743
                     /locus_tag="PLH31_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89344.1"
                     /translation="MQDSGTELAQDLRDLTLQTLCRKYAGEASIHRNILMSRGTGVTG
                     TWRDFRAFLTDIGPRPTPEHVLVLINRHERTYGPGRARWMTPEEQAAHEAEFDRIQAE
                     KKREEQLLLHQSAAMKRAKDPKAASFGQWTPMQGQQVAYSDVAKKLNIPIAALSKTMA
                     DGRSADELLKRASSAGELINQDATWLPPDPKRKAGFFEAFRIWHMQVQPQFSRAATPT
                     FLFLYIALPVMKQCRDELMDLDLWNPLGQRAMSARDNHLSWKKYCEFMP"
BASE COUNT          440 a          908 c          924 g          471 t
ORIGIN      
        1 acgggcggcg gcgatcttgg tggccatgcc gccggtcccg acgcccgagg cggcgttggc
       61 gccgccagcc atggcttcga tctgcggcgt gatctcggcg atcgtcgcca gatggctggc
      121 gtttggatcg cggcgcggat cgacggtgta gaggccgtca atgtcggaca gcagcaccag
      181 caggtcggcc ccggccagct gggcgacgcg ggcggccagg cggtcattgt cgccgtagcg
      241 gatttcctct gtgacgacgg tgtcgttctc gttgaccacc ggcaccacgc ccaagcccag
      301 cagggtctcg accgtggcgc gggcgttcag ccagcggcgg cgggtctcgg tgtcgtcgcg
      361 ggtcatcagg atctgggcgg cgccgatgcc atgcggctcg aacgcctctt cccaggcgcg
      421 catcagcagc gactggccgg ccgcagcggc ggcctgcttc tcgggcaggg tcagcgactt
      481 gcgaccggtc aggcccaggc gtcggcggcc cagggcgacg gcgccggacg agaccacgac
      541 cacctgctgg ccccgggccc gcaggcgggc ggcgtcatcg cagaaggccc tcagccaggc
      601 atggttggcc gcgccggtgt tggcgtcgac cagcagggcc gagccgacct tgaagacgat
      661 ccgccgggcg gacgccagcg gccccggatt ttggctcacg gggtccagcc cccgggggtc
      721 tccgggacgt ggtcttcttc ttcgtcgatt tcctcgatct cgtcgccgcg acggatgcgg
      781 acttcgcgat aggcggcgcg gagcagctcc ttcacgccct tgccggacac acccgagacc
      841 agacgtggct tgacgccggc cacggcttcc agctcggcgg ccttttccgc gatcgtgtcc
      901 tcgtcgaggg cgtcgatctt gttgagggcc aggatctcgg acttgtcgcc cagctcgtcg
      961 ccataggctt caagttcgcc ccggatcgtg gtccaggcgg cgccgatgtc gtcctgggtg
     1021 gcgtcgacca gatggatcag aaccgccgac cgctcgacat ggcccaggaa acgggtgcca
     1081 aggcccgcgc cttcagaggc gccctcgatc aggccgggaa tgtcggccag cacaaaacgc
     1141 tcgctggttg acaggtcgac cacgccgagg ttgggggtca gggtggtgaa gggatagtcg
     1201 gcgatcttgg gcttggcggc cgtggcggcg gccagaaagg tcgacttgcc ggcattgggc
     1261 agacccacca gaccgacgtc ggcgatcagc ttcaggcgca gccagaccca gcgctcttcg
     1321 ccttcctggc caggattggc gaatttgggc gcctggttca ccgggccctt gaagtgcagg
     1381 ttgccccagc cgccattgcc gcccttgcac aggcgcacgg ccatgccggc cgtatcgagg
     1441 tcggcgatca gggtttcctt gtcctcttcc agcacctggg tgccgacagg gactttcagg
     1501 gtgatgtcgg cgccggccgc gccatggcgt ccgcggccca tgccgtggat cccggtctcg
     1561 gccttgaagt gctgctggta gcggtagtca atcagggtgt tgaggccgtc cacggcctcg
     1621 atccagatgt cgccgccacg gccgccgtcg ccgccgtcgg ggccgccgta ttcaatgtac
     1681 ttctcgcgtc ggaacgagac cgcgccgccg ccgccgttgc cggagcggat aaagatcttg
     1741 cactggtcca gaaatttcat ctgcgtcttt tgtcgcaagg cgcttgctgc gtcgagccga
     1801 aatggcgtcg caggcccgca ggctgccgtt cagagcgctg tttgtttctg tgacgaaatg
     1861 ttaaaacacg ttgtgcgaca aagggtcctg cagtgaaacc gtcgtttttg gcggcggaag
     1921 gggaggctgg ctttgacgac gatgcaagac agcggaacgg aactggcgca ggatcttcgc
     1981 gatctcacgc tccagacgct gtgtcgcaaa tatgccggcg aagcctccat ccatcgcaac
     2041 atcctgatga gccgcgggac cggcgtgacc ggcacctggc gcgatttccg ggccttcctg
     2101 accgacatcg gcccgcgccc cactccggaa catgttctgg tgctgatcaa tcgccatgag
     2161 cgcacctatg gtcctggtcg cgcccgctgg atgacgccgg aagaacaggc ggcccatgag
     2221 gccgagttcg accgcatcca ggccgagaag aaacgcgagg agcagcttct gctgcaccag
     2281 tcggccgcga tgaagcgcgc caaggacccc aaggccgcct catttggcca gtggacgccg
     2341 atgcagggcc agcaggtggc ctatagcgac gtcgccaaga agctgaacat tccgatcgcg
     2401 gccctgtcaa agaccatggc cgatggccgc tcggccgacg aactgctcaa gcgcgcctct
     2461 tccgccggcg agttgatcaa ccaggacgcc acctggctgc cgcccgatcc caagcgcaag
     2521 gccggcttct tcgaagcctt ccgcatctgg cacatgcagg tgcagccgca gttcagccgc
     2581 gccgcgacgc cgaccttcct gttcctctat atcgccctgc cggtgatgaa gcagtgccgg
     2641 gacgaactga tggacctgga tctctggaac ccgctgggcc agagggccat gagcgcgcgc
     2701 gacaaccacc tgtcgtggaa gaaatactgc gagttcatgc ccc
//
LOCUS       DAOMEO010000351         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3989_length_2740_cov_2.356797, whole genome shotgun sequence.
ACCESSION   DAOMEO010000351 DAOMEO010000000
VERSION     DAOMEO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3989_length_2740_cov_2.356797"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2125)
                     /locus_tag="PLH31_08805"
     CDS             complement(<1..2125)
                     /locus_tag="PLH31_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR89345.1"
                     /translation="MPATSSSDATLLAPSSFGEAEAARWFFENANDLFAVVTPDGRFS
                     VVNPAWTVLTGWTPEDLIGQPSIKFVHPDSHAELIDTGRRIRETGFAVNRLRVLCKTG
                     CTVWLEGRSRRGPNGEMVGTLHDVSLEVAATRTREILAEAAGVGLWSYDPLTERIDWT
                     PDALTATGLSPADLETIDAFLARLDDDQREAVFSIFQGAVRTGASGAVEFRLRGRAGQ
                     WFTLRSTFRADPLDGGLFALKGISQNVTDVVRSRDAALWGERRARQLVEEAPFAVAVY
                     DLDLRLRLASPRFLDLFKSSEADVIGKSLQELTHGGRRRFVSAVTRALSGETVTRRED
                     RLRDAEGVEHTLRWEARPWRDATGAIAGVITYMDDVTALADARREAQVNARRLRIALG
                     AARAGVYEIDHAGQGFWGSPEFHRMLGRKIHYPDVREAVWPMIHPDDRAALMAAADSW
                     RRTGDWGALSVDARLITASGQARWMRIFHEVRNDAAGRARKAFGMILDIDEAKRAELA
                     LVQAERAAQAASEAKAQFLANMSHEIRTPMNGVLGVMHLLRRQGLPGDASELLDEALG
                     CGQMLSTLLDDVIDISRIEAGRLEISCEPVEPRELLRGVARLMAGQAEHKGLTLEIDA
                     PDLGWIDTDPTRLRQALFNLIGNAVKFTLQGSVTVRARRLDAGSGPQIAFDVIDTGVG
                     VPKEAQARLFERFEQGDASTTRRFGG"
     gene            complement(2229..>2740)
                     /locus_tag="PLH31_08810"
     CDS             complement(2229..>2740)
                     /locus_tag="PLH31_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HQR89346.1"
                     /translation="AIILAALERNPLFVSAALPRTILSPMFNRYGAGMGFGDHIDNSV
                     RRDPVTGQTLRTDLSATLFLSEPEDYDGGELVVDDLYGSHAVKLSAGDLILYPASSLH
                     HVTEVTRGTRTASFFWIQSLVRDDARRTLLDMDVAIQRLSQAMGAADPSILTLTGTYH
                     NLLRQWAEV"
BASE COUNT          439 a          931 c          945 g          425 t
ORIGIN      
        1 atccgccgaa gcgccgggtc gtgctggcgt cgccctgctc gaaccgttcg aagagccgcg
       61 cctgggcctc cttgggcacc ccgaccccgg tatcgatgac gtcgaaggcg atctgaggcc
      121 cagaccccgc atccaggcgc cgcgcccgca cggtgaccga gccctgcagg gtgaacttca
      181 ccgcattgcc gatcaggttg aacagcgcct gacgcagtcg ggtagggtcg gtgtcgatcc
      241 agcccaggtc tggcgcatcg atctccaggg tcaggccctt gtgttcggcc tgcccagcca
      301 tcagccgtgc gacccctctc agcagctcgc gcggttcgac aggctcgcaa ctgatctcga
      361 gccgccccgc ctcgatgcgc gaaatgtcga tcacgtcgtc cagcagggtc gacagcatct
      421 ggccgcagcc cagggcctcg tccagcagtt cggacgcatc ccccggcagg ccctgccgcc
      481 tcagcaggtg catgacgccc agcacgccgt tcatgggcgt gcgaatctcg tggctcatat
      541 tggccaggaa ctgggccttg gcttcgctgg ctgcctgggc ggcgcgctcg gcctggacga
      601 gagccagctc ggcgcgcttg gcctcgtcga tatcgaggat catgccgaag gccttgcgcg
      661 cccggccggc agcgtcgttg cggacctcgt ggaagatgcg catccagcgt gcctgtcccg
      721 acgcggtgat caggcgcgca tccaccgaca gggcccccca gtctcccgtg cgccgccagc
      781 tgtcggcggc ggccatcagg gccgcgcggt catcgggatg aatcatcggc cagacggctt
      841 cgcggacgtc tggatagtgg attttccgac ccagcatgcg gtggaactcg ggcgaacccc
      901 agaagccctg gcctgcatgg tcgatctcgt agaccccggc ccgcgccgcg cccagggcga
      961 tccgcaaccg gcgcgcattg acctgcgcct cacgccgggc gtcggccagg gcggtgacgt
     1021 cgtccatgta ggtgatgacg ccggcgatcg ccccggtcgc gtcgcgccac gggcgtgctt
     1081 cccaccgcag ggtgtgctcg acgccctccg cgtcgcgaag gcgatcttcg cggcgggtca
     1141 cggtctcgcc ggacagcgcc cgggtgacgg ccgagacgaa ccggcggcgc ccgccatggg
     1201 tcagctcctg cagcgatttg ccgatgacat cggcctcgct cgacttgaag agatccagga
     1261 accgcgggct ggctaggcga agtctgaggt cgaggtcata gacggccacc gcgaagggtg
     1321 cctcctcgac cagctgccgg gcccggcgtt cgccccacag ggcggcatcg cgactacgga
     1381 cgacgtcggt gacgttctgc gaaatgccct tcagggcgaa caggccgcca tccagcgggt
     1441 cggcgcgaaa ggtcgagcgc agggtgaacc attggccggc gcggccgcgc agccggaact
     1501 ccaccgctcc ggacgcgccg gttcggacag cgccctgaaa tatgctgaac accgcctcgc
     1561 gctggtcgtc gtccagccgc gccaggaagg cgtcgatggt ctccagatcg gcgggagaca
     1621 ggccggtggc ggtcagggcg tcgggcgtcc agtcgatgcg ttcggtcagg ggatcgtagc
     1681 tccacaggcc gacgccggca gcctcggcaa ggatctcccg ggttcgggtg gcggccacct
     1741 cgagcgagac gtcgtgcagc gtaccgacca tctcgccgtt gggaccgcgg cgcgagcggc
     1801 cttcaagcca gaccgtgcag cccgtcttgc acaacactcg cagccggttg acggcgaagc
     1861 cggtctcgcg gatccggcga cccgtatcga tcagctcggc atggctgtcg ggatggacga
     1921 acttgatgct gggctggccg atcaggtcct cgggcgtcca gcccgtcagc accgtccagg
     1981 ccggattgac caccgagaac cgtccgtcgg gcgtgaccac ggcgaacagg tcgttggcgt
     2041 tctcgaaaaa ccaacgcgcc gcttccgcct cgccgaacga cgaaggcgcg agcagggtcg
     2101 cgtcggatga cgaggtcgca ggcatcaggg ctccggaaga tcgcgagcgg atcgtggggg
     2161 ccattggtaa acttatggtt ggcgtcgcac atcattgttc gccgagcatg ctcgctgaag
     2221 ggtcgggctc agacctccgc ccattgccgc agcaggttgt gataggttcc ggtcagggtc
     2281 aggatcgacg gatcggccgc gcccatcgcc tgcgacaggc gctggatggc gacgtccatg
     2341 tccagcaggg tccgccgggc gtcgtcgcgc accagactct ggatccagaa gaagctggcc
     2401 gtgcgggttc cgcgcgtcac ctcggtcaca tggtgcaggc tcgaggcggg atagaggatc
     2461 aggtcgccgg ccgacagctt gacggcgtgg ctgccataca ggtcgtcgac caccagctcg
     2521 ccgccgtcat agtcctcggg ttccgacagg aacagcgtgg ccgacaggtc ggtgcgcagg
     2581 gtctggccgg tgaccggatc gcggcgcacc gaattgtcga tatggtcgcc aaagcccatg
     2641 cccgcgccat agcggttgaa catcggagac aggatcgtgc ggggcaaggc cgccgaaacg
     2701 aacagcgggt tgcgttccag cgccgccagg atgatcgccc
//
LOCUS       DAOMEO010000352         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_3995_length_2736_cov_2.836255, whole genome shotgun sequence.
ACCESSION   DAOMEO010000352 DAOMEO010000000
VERSION     DAOMEO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_2736_cov_2.836255"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLH31_08815"
     CDS             complement(<1..661)
                     /locus_tag="PLH31_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HQR89347.1"
                     /translation="MQTRLTRPLVLAALTLALGACATAAPERSGFLSGYEGLAARDNG
                     GRARIREFRDEAALNSVRRVALTPAVLSEGADSRSPLTPDERRAVLREVDAQLCFELS
                     ERFEIVGAEQADADVRAAITWFEPTGRIASAASAASGFFIPGPIGLRAPGTLGGLGVE
                     AEMRKTSDQRQIAAIAWARRAMAVGTDNPSLSRLGDALQFAEPFADTAAAVMTPKGLK
                     SR"
     gene            complement(766..>2736)
                     /gene="purL"
                     /locus_tag="PLH31_08820"
     CDS             complement(766..>2736)
                     /gene="purL"
                     /locus_tag="PLH31_08820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQR89348.1"
                     /translation="GVIDIGDGDAIIFKMESHNHPSYIEPYQGAATGVGGIMRDVFTM
                     GARPIALLNALRFGDPSHPKTKRLVDGVVAGIAGYGNCVGVPTVAGETNFHRGYNGNI
                     LVNAMCVGHAEADKIFYSAAPGAGLAVVYFGSKTGRDGIHGATMSSAEFSEDSEEKRP
                     TVQVGDPFAEKLLIEATLELMATGAVAAIQDMGAAGLTSSSVEMAGKGGVGIELNMDM
                     VPQRETGMTAYEMMLSESQERMLAVLKPGREHEGHAIFEKWGLDAAVIGYTTDTGRLV
                     LKHHGETVCDVPLAPLFDDAPLYDRPWVQPALHPRLDPGQVPAPADWNAAVLKVVGCP
                     DMASKRWLWEQYDRHVMADTLEDSATGCDAGIVRIHGKGKAIAVTSDVTPRYVQNDPY
                     EGGKQAVAEAWRNLTAAGSLPIAITDNLNFGSPEKPETMGQIVRATDGMAEACRALDF
                     PVVSGNVSLYNETNGVAIPPTPTVGAVGLLEDYDLRMGFGNVAEGDTLVLIGDTNGEL
                     GASIYLREILGREDGAPPPVDLAAERKHGDFVRGLIPTGLIAGLHDLSDGGLLIAAAD
                     IALASKVGITLNASSNQNAHPYLFGEDQARYLIATPDPDAVLAAAKEAGVHANVAGVA
                     GGDAFASKDLFSVPLDTLRAAHEAWMPGFMGG"
BASE COUNT          401 a          911 c          973 g          451 t
ORIGIN      
        1 cgcgggactt caggcccttg ggggtcatga ccgccgcggc cgtgtcggcg aagggttcgg
       61 caaactgcag ggcgtcgccc agtcgcgaca gggatggatt gtccgtaccc accgccatcg
      121 cgcgacgcgc ccacgcaatc gccgcgatct ggcgctgatc gctggtcttt cgcatctccg
      181 cctcgacgcc cagtccgccc agggtgccgg gcgcgcgcag gccaatgggg ccggggatga
      241 agaaccccga cgccgccgaa gcggcggaag caatccggcc ggtgggctca aaccaggtga
      301 tggccgcgcg gacgtccgcg tcggcctgtt cggcgccgac aatctcgaac cgctcggaga
      361 gctcgaaaca cagctgcgca tccacttcgc gcagaacggc ccgccgctca tccggggtca
      421 ggggcgagcg gctgtcggcg ccttcggaca gcaccgctgg cgtcagggcg acgcggcgca
      481 cgctgttgag cgcggcctca tcccgaaact cgcggatgcg ggcgcggccg ccgttgtcac
      541 gcgccgccag gccttcatag ccgctcagaa agccggagcg ctcgggcgcc gccgtcgcgc
      601 aggcgcccag cgccagggtg agggcggcaa gcaccagggg gcgggtcaat cttgtttgca
      661 tgatggagtg tcccgctcaa aggtggccgc gtcgaggcgg ctccaagtca tacacttaga
      721 gcgcgattgc cgccgaaacc gcatgtactt tcggcgatcg ccgctttagc cgcccatgaa
      781 accgggcatc caggcctcgt gagctgcacg cagcgtgtcc aggggcacgc tgaagaggtc
      841 ttttgacgca aacgcgtcgc ctccggccac gccggccaca ttggcatgga cgcccgcctc
      901 cttggccgcc gccaggaccg cgtcggggtc cggcgtggcg atcaggtagc gcgcctggtc
      961 ttcgccaaac agataggggt gggcgttctg gttgctggag gcgttgagcg tgatcccgac
     1021 cttgctggcc agggcgatat cggccgccgc gatcagcagg ccgccgtccg acaggtcgtg
     1081 cagaccggcg atcaggccgg tggggatcag gccgcgcacg aagtcgccgt gcttgcgctc
     1141 ggcggccagg tccaccggcg gcggggcgcc gtcctcgcgg cccaggatct cgcgcagata
     1201 gatcgacgcg ccaagttcgc cgttggtgtc gccgatcagc accagggtgt cgccctcggc
     1261 cacgtttccg aagcccatgc gcaggtcgta gtcttccagc aggcccacgg cgccgaccgt
     1321 gggggtgggc ggaatggcga cgccgttggt ctcgttgtag aggctgacat tgccgctcac
     1381 gaccgggaag tccagcgcgc ggcaggcttc ggccatgccg tcggtggcgc ggacgatctg
     1441 gcccatggtc tcgggctttt cggggctgcc gaagttgagg ttgtcggtga tggcgatcgg
     1501 cagcgagccg gcggccgtca ggttgcgcca ggcctcggcc acggcctgct tgccgccctc
     1561 ataggggtcg ttctgcacat agcgcggggt gacgtcgctg gtcacggcga tggccttgcc
     1621 cttgccgtgg atgcgcacga tgccggcgtc gcagccggtg gcgctgtctt ccagggtgtc
     1681 agccatcacg tggcggtcgt actgctccca cagccagcgc ttggaggcca tgtcggggca
     1741 gccgaccacc ttcagcacag cggcgttcca gtcggcgggg gcggggacct ggccggggtc
     1801 gaggcgcgga tgcagcgcgg gctggaccca cgggcgatca tacagagggg cgtcgtcgaa
     1861 cagaggggcc aggggcacgt cgcagacggt ctcgccgtga tgcttcagaa ccaggcggcc
     1921 ggtgtcggtg gtgtagccga tgacggcggc gtccaggccc cacttttcga agatggcgtg
     1981 gccttcgtgt tcgcgccctg gcttcagcac cgccagcatg cgctcctggc tttccgacag
     2041 catcatctcg tatgccgtca tgccggtttc gcgctgcggc accatgtcca tgttcagctc
     2101 gatgccgacc ccgcccttgc cggccatctc gaccgacgac gaggtcaggc ccgccgcgcc
     2161 catgtcctgg atggcggcca cggcgccggt ggccatcagt tccagcgtgg cttcgatcag
     2221 cagcttttcg gcaaaggggt cgccgacctg gacggtgggg cgcttttcct cggagtcttc
     2281 ggagaactcg gccgacgaca tggtggcgcc gtggatgccg tcgcggccgg tcttggagcc
     2341 gaaatagacc acggccaggc ccgcgcccgg cgcggcggag tagaagatct tgtccgcctc
     2401 ggcatggccc acgcacatgg cgttgaccag gatgttgccg ttatagccgc gatggaaatt
     2461 ggtctcgccc gccacggtgg gcacgcccac gcagttgccg tagccggcga tgcccgcgac
     2521 cacgccatca accaggcgtt tggtcttggg atggctggga tcgccgaacc gtagggcgtt
     2581 cagcagggcg atcggccgcg cgcccatcgt gaacacgtcg cgcatgatgc cgcccacgcc
     2641 cgtcgccgcg ccctgatagg gctcgatata ggaggggtgg ttgtggctct ccatcttgaa
     2701 gatgatcgcg tcgccgtcgc cgatgtcgat gacgcc
//
LOCUS       DAOMEO010000353         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4001_length_2732_cov_2.128502, whole genome shotgun sequence.
ACCESSION   DAOMEO010000353 DAOMEO010000000
VERSION     DAOMEO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_2732_cov_2.128502"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..663
                     /locus_tag="PLH31_08825"
     CDS             202..663
                     /locus_tag="PLH31_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89349.1"
                     /translation="MTAYTDAQVAALAERLLDHSLPKAEWTHAAHLTATLRLVRTRDA
                     GLERDLPEIIRRYNVAVGGVNDDASGYHETITQAYLTAIRAFVATLPPGLGDGEASAR
                     LLATPLGDKAWPLGFWSRERLFSVEARRTWMEPDLRGLDQAPPFLAAARLD"
     assembly_gap    675..684
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            720..1385
                     /locus_tag="PLH31_08830"
     CDS             720..1385
                     /locus_tag="PLH31_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR89350.1"
                     /translation="MIVVHHLNNSRSQRVLWLLEELGVPYEVKRYERDAVTMLAPPAL
                     LAIHPLGKSPVITDGDKTIAETGAIVDYVIETYGQGRLIPAAGTAERLRYTYWLHYAE
                     GSAMTPLLLKLVFTALPTRAPGLLKGLVKAIASKAQTGFIDPQVKTHVDYWEAELARS
                     AWFAGQDFTAADIMMSFPLEAAAARAGAASRPHVKAFLEAIHARPAYRAALTRGGPYD
                     YAS"
     gene            1587..1868
                     /locus_tag="PLH31_08835"
     CDS             1587..1868
                     /locus_tag="PLH31_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89351.1"
                     /translation="MAIFSGLFAAAAVNAAPRDPSRVAAIFPPWWSPAQAVAAAGSAG
                     DIAATGSTPFILILRGDPADLAQRARSVGALLVLDPDLAGLCAPKDARP"
     gene            1865..>2732
                     /locus_tag="PLH31_08840"
     CDS             1865..>2732
                     /locus_tag="PLH31_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR89352.1"
                     /translation="MTLAFSSLDAQRQVAGKLIVLLTWVMVAVVLGARAAVHEPVLSL
                     AIASLITAGAATLAIRIPGSLGRSLSGVALMAQVSLLVAAMGGHAWQTDMHMAYFAAL
                     ALLVVYCDWKVIAAAAATVAVHHLGLSFLLPQAVFPGSASLGRVVVHAVILVVEAGAL
                     IATTFSVNAMFTTANKARAEAEQAVEAVRAANDVAEGARRSEETNRAQTAQAEAVAER
                     QRAEAVASIAAGLADLAKGDLTVRLTTRFAQAYEALRTDFNAAAERVQAAMQAISQTT
                     RAVSAGADEIAEA"
BASE COUNT          418 a         1014 c          857 g          433 t
ORIGIN      
        1 gcggcggcat ccggtgcgac accgccgagt ccgccacccc cgagctggac gccgccgccc
       61 tggcgctgag cggcctctac aaggtggaca tgaccgccga aaaggaccgc gtcggctaca
      121 gcttcaagct gccgttccgg tttaccccgc cggcgcctga tggctcttga aacgggcgcg
      181 caaaccctcg atccagaggc catgacagcc tataccgacg cccaagtcgc cgccctcgcc
      241 gagcgtctgc tggatcattc gctgcccaag gccgagtgga cccacgccgc gcacctgacc
      301 gccaccctgc gcctggtccg cacccgcgac gcagggctgg agcgcgacct gccagagatc
      361 atccgccgct acaatgtcgc ggttggcggg gtgaatgacg atgcgagcgg ctatcacgag
      421 accataaccc aggcctatct gaccgccatc cgcgccttcg tcgccaccct gccccccggc
      481 ctcggcgacg gcgaagcctc cgcccgcctg ctggccaccc cgctgggcga caaggcctgg
      541 cccctgggct tctggtctcg tgagcggctg ttcagcgtgg aggcccgccg gacctggatg
      601 gagccagacc tgcggggact ggaccaggct ccgcccttcc ttgcggcggc gaggcttgac
      661 tgacgcgggc ctcgnnnnnn nnnnccgtgc gacgctgagc gccagaccgg agaagcccca
      721 tgatcgtcgt ccatcacctg aacaactccc gcagccagcg ggtcctctgg ctgctggagg
      781 aactgggcgt tccctacgag gtcaagcgtt acgagcgtga cgccgtcacc atgctggccc
      841 cgcccgcatt gctggccatc cacccgctgg gcaagtcgcc ggtgatcacg gacggcgaca
      901 agaccatcgc cgagaccggc gccatcgtcg actatgtcat cgagacctat ggccagggcc
      961 ggctgatccc cgccgcaggg accgccgagc ggctgcgcta tacctactgg ctgcactatg
     1021 ccgagggctc ggcgatgacg ccgctgttgc tgaaactggt gttcaccgcc ctgcctacgc
     1081 gggcgccggg cctgctgaag ggcctggtca aggccatcgc cagcaaggcg cagaccggct
     1141 tcatcgatcc gcaggtcaag acccatgtcg attactggga ggccgaactg gcccgctcgg
     1201 cctggttcgc cggccaggat ttcaccgccg ccgacatcat gatgagtttc ccgctggagg
     1261 ccgccgccgc gcgcgccgga gcggcgtccc ggccgcacgt gaaagcgttt ctggaggcga
     1321 tccatgcccg ccccgcctat cgcgcggccc tgacccgtgg cggcccttac gactacgcga
     1381 gttgagaagg acttgcagtt tgtcgaagcg ctaaaatgtc gcgcaaccgc ctgatttaag
     1441 atttgggaaa ccaaagttca gcactggtga gagcacggct gcgggaaatt cttcccggac
     1501 cacccaggca cgattgaatc ttggcgaaga ccacgacaat gtttggaaag acggccgcta
     1561 acattgcgcc gatccttgcc acaactttgg cgatattttc aggcctgttc gcggcggcgg
     1621 cggtcaacgc cgccccgcgc gacccgtcgc gcgtcgcggc catcttcccg ccctggtgga
     1681 gtcccgccca ggctgtcgcg gcggccggct cggccggcga catcgccgcg accggctcca
     1741 cgccgttcat cctcattctc cgcggtgatc cggcggatct ggcgcagcgc gcccggtctg
     1801 tcggcgcgct gctcgttctt gaccccgacc tcgcaggcct ctgcgcgcca aaggatgctc
     1861 gtccatgacc ctcgcgtttt ccagcctaga cgcccaacgc caggtcgccg gcaagctgat
     1921 cgtcctgctg acctgggtga tggtcgccgt tgttctgggc gcccgggctg cggtgcatga
     1981 accggtcctt agcctggcca tcgcctccct gatcacggcc ggcgccgcca ccctggccat
     2041 ccggatcccc ggctcgctgg gccgatcgct gtcgggcgtc gccctgatgg cccaggtctc
     2101 cctgctggtc gcggccatgg gcggtcacgc ctggcaaacc gacatgcaca tggcctattt
     2161 cgcggccctg gccctgctgg tggtctattg cgactggaaa gtcatcgccg ccgccgccgc
     2221 caccgtggcc gtgcaccatc ttggcctgag cttcctgctg ccccaggccg tcttccccgg
     2281 ctcggccagc cttggtcggg tggtggtcca cgccgtgatc ctggtcgtcg aggccggcgc
     2341 cctgatcgcc accaccttca gcgtcaacgc catgttcacc accgccaaca aggcccgcgc
     2401 cgaggccgag caagccgttg aagccgtccg cgcggccaat gatgtcgccg agggcgcccg
     2461 acgctccgag gaaaccaatc gcgcccagac cgcccaggcc gaagcggtcg ccgagcgcca
     2521 gcgcgccgag gccgtcgcct ccatcgccgc aggtctggcc gacctggcca agggcgacct
     2581 gaccgtccgc ctgacgacgc gcttcgccca ggcctatgag gcgctgcgaa ccgacttcaa
     2641 cgccgccgcc gagcgggtgc aggcggccat gcaggccatc tcgcagacca cccgggccgt
     2701 cagcgccggg gccgacgaaa tcgccgaggc ct
//
LOCUS       DAOMEO010000354         2728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4002_length_2728_cov_2.895249, whole genome shotgun sequence.
ACCESSION   DAOMEO010000354 DAOMEO010000000
VERSION     DAOMEO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4002_length_2728_cov_2.895249"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..325
                     /locus_tag="PLH31_08845"
                     /pseudo
     CDS             35..325
                     /locus_tag="PLH31_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
     gene            complement(390..638)
                     /locus_tag="PLH31_08850"
     CDS             complement(390..638)
                     /locus_tag="PLH31_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89353.1"
                     /translation="MEIREVLARNLRAARRAKGLSQEELAHQAGIDRTYISSLERSVY
                     GASIDVVDRLASVLELDAADLLKRPAADLQDPADGSDE"
     gene            783..1052
                     /locus_tag="PLH31_08855"
     CDS             783..1052
                     /locus_tag="PLH31_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HQR89354.1"
                     /translation="MRSLMAETFEDTAPSNPELTGYDRAHIRLYARLLDATADGADWQ
                     EIVSVLFGLDPAREPERARHVHDSHLARARWIAAGGYKDLLRRPV"
     assembly_gap    1279..1288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1410..1892
                     /locus_tag="PLH31_08860"
     CDS             1410..1892
                     /locus_tag="PLH31_08860"
                     /inference="COORDINATES: protein motif:HMM:NF021581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HQR89355.1"
                     /translation="MDQNILWSPIVDPAVVLLAQCPRFIPDVPAVALEGSIELRESAE
                     GAHAARGPISSSTQLLFLPGAYPDAPLAAVIPLDADMVGRVEALTRFWRSTQGRPAIP
                     DTRITSQQRARLRAMMLAADGRANGASYRDVAMVLYGHKRIASESWKTSALRDRVIST
                     "
     gene            1889..2365
                     /locus_tag="PLH31_08865"
     CDS             1889..2365
                     /locus_tag="PLH31_08865"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HQR89356.1"
                     /translation="MKSGSLSPFKRFAFELRDIIRRQPLPGYTLFLEVDGRARVLLAF
                     EPACEQAVNPFVLSGTREHVPSRTGLSCYREPKQGLSLSTRSGNRGANLESNRESNSV
                     ERADDVHDRIRRGRKRARLMPSESQHLPSFRKDGFQAPDPLSTSAPRPSRTSGESR"
     gene            2362..>2728
                     /gene="parA"
                     /locus_tag="PLH31_08870"
     CDS             2362..>2728
                     /gene="parA"
                     /locus_tag="PLH31_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HQR89357.1"
                     /translation="MIVALLNQKGGVGKTTLALHLAGAWASRGKRVTLIDADPQGSAL
                     DWSQQRVRDGGSRLFGVVGLARDTLHRKAPELARDADHVVIDGPPRIAGLMRSALLAA
                     DLVLIPVQPSPLDGWASAEM"
BASE COUNT          480 a          903 c          820 g          515 t
ORIGIN      
        1 ttctgctcaa aatgacagtt gacggctgac ctaaaacgag ttcaccggcg acatcatcac
       61 tctcggtgtc caggcgaaag gcgtgcgcat cgttcccgac gctcgggcca ccggcgagaa
      121 cgctcccagc caccgggtcc tggtcggccg catcgagatc ggtgccgcct ggtccaagcg
      181 ctccaacgag ggccgcgact atctgggcct caagctcgat gatccgagct taaacgctcc
      241 gatctacgcg aacctgttca gcgacgagga cggcgaaggc ttctcgctga tctggtcccg
      301 ccccacaggt cgacgtggcg agtgaatctt gcgaggccct ggccgccacg gccggggcct
      361 tttcaatagg cctaccaact gcgcgactgc tattcgtccg acccatcggc tggatcctgt
      421 aaatccgctg ccgggcgctt caataaatct gcagcgtcca actcaaggac ggacgccaat
      481 cggtcaacca cgtcgatact agcgccatac acactccgct ccagggagct gatatatgtc
      541 cggtcgatgc cagcttgatg ggctagctct tcctgcgaca ggcctttggc gcgcctcgcg
      601 gcccttagat tgcgggcgag gacctctcgg atctccatga tcgagagagc atcgccttga
      661 tgagtattct accacggagt attctctaca aactgcagcg gcgacccctc cgcagcattg
      721 gaaagtgttg accacaaaag aggggtaggg tgttggtgga tgcccccagc cgagggagga
      781 aactgaggag cctaatggcc gagacgttcg aggacaccgc accgtccaat cctgagctga
      841 cgggttacga ccgggcgcac atcaggctct atgcacgcct gcttgatgcc actgcggacg
      901 gcgccgattg gcaggagatc gtctccgtgc tgttcggcct cgatcctgcc cgggaacccg
      961 aacgcgcccg gcatgtccac gacagtcatc tcgcccgggc ccgatggatc gctgccggcg
     1021 gctataagga cctcctccgc cgtcccgtct gatacgacga cccgttggga gggtgatgca
     1081 gtagccgcat cacgtgatgc gcactcggtg gcttcccagg atatctacta tccctgattg
     1141 tcccgctcga cgcgtacgca tctgacgcgg agacgagtga tgaagcccga cacatcgcgc
     1201 tggcgcgaag aggccagcta cgactatttc gacgcgctgt cggtcgaggg cctcgcgtgg
     1261 gaatgtctgc gccgggatnn nnnnnnnnga agcctaccag gcgcactacc agtccctgat
     1321 gaaagccggc gtcttcgccg atccgctccc tcgggaggtg gagcagcgct ggggtttgcg
     1381 atttcgccgc gcagcccacg ctctcttcgt tggaccagaa catcctgtgg tcaccgatcg
     1441 tcgatccggc tgtcgtgctt ctcgcccaat gcccccgatt catccccgac gtgcccgctg
     1501 tcgcgctcga gggctccatc gagctgcggg agagtgcgga gggagcacat gcggcccgcg
     1561 gccctatctc ttcctcgacc cagttgctgt tcctgcccgg cgcttatcct gatgcgccat
     1621 tggcggcggt gatcccactg gatgcggaca tggtggggcg cgtggaggct ctgacccgct
     1681 tctggcgttc cacgcaaggc cgcccggcga tccccgacac gcgcatcacc tcgcaacagc
     1741 gcgcccgatt gcgggccatg atgctggcgg ccgatggccg ggccaatggg gccagctatc
     1801 gcgacgtggc catggtcctc tacggccaca agcggatcgc gtccgaatcc tggaagacct
     1861 ccgcgctgcg cgacagggtc atctccacct gaagtcgggc agcctctcac cctttaagcg
     1921 cttcgccttc gagcttaggg acatcattcg ccgccagccg ttgcccggct acaccctgtt
     1981 tctggaagtc gacgggcgcg cacgcgtgct gctggcattt gagccggcct gtgaacaggc
     2041 tgtgaatccc ttcgtgctat cgggaacccg agagcacgtg ccatcaagaa ccggtctctc
     2101 gtgctatcgg gaaccgaaac agggattaag tctaagcacc agaagcggaa atcgcggtgc
     2161 taacttagag tctaacagag aatctaactc tgttgagcgc gcggacgatg tgcatgatcg
     2221 catccgccgc gggcgaaagc gcgcccgtct catgccgtca gagagtcaac atcttccgtc
     2281 tttccgtaaa gacggatttc aggcgccaga tcctctctcc acatccgccc cacgtccctc
     2341 tcgtacatcg ggagaatccc gatgatcgtc gcgctgctca atcagaaggg cggcgtcggc
     2401 aagacgacgc tcgccttaca tctcgctggc gcttgggcga gccgcggcaa gcgcgtcacg
     2461 ctgatcgatg cggatccgca gggttcggcg ctcgattggt cgcagcagcg cgtgcgcgat
     2521 ggcggttccc ggctcttcgg cgtcgtcggt ctcgcccgcg acacgctgca tcgtaaggca
     2581 ccggaactcg cgcgggacgc cgatcatgtt gtgatcgatg ggcccccccg catcgccggc
     2641 ctcatgcgct cggcgctcct cgccgccgac ctggtgctga tcccggtgca gccttcgccc
     2701 ctcgatggct gggcctccgc cgagatgc
//
LOCUS       DAOMEO010000355         2722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4014_length_2722_cov_2.974878, whole genome shotgun sequence.
ACCESSION   DAOMEO010000355 DAOMEO010000000
VERSION     DAOMEO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4014_length_2722_cov_2.974878"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..>2722
                     /locus_tag="PLH31_08875"
     CDS             224..>2722
                     /locus_tag="PLH31_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89358.1"
                     /translation="MKQYKTIRRRLLGSSMICGVAIAVLATGQASAQTAAPEPESTEI
                     EAVVVTGSLFRRTDTETPSPVTVMTSESLARAGITTATDAIRSVSADGAGSIGTGFQS
                     GFSAGGSAVSLRGLGVSSTLVLVDGLRSANFPINDDGHNAYVDLNSIPFSLIDRIEVL
                     KDGASSSYGADAIGGVVNLILKKQFVGVAGSAEFGESDRGDAEHRQFDLTLGYGDYAQ
                     SGWNFYVNGEYQKDGRVTNHSRGFPYNTQDLSRIGGLDQNTADSTLTTATPTAVVTRV
                     SQTDLNNPLSGMIGAPLTNQYQALNLNCANGSYTVTTGSARGTSCKYDLVDLYNQIAP
                     FQERYAVNGRLSVRLSDNIEGYITGSFSHDEVRIKNQPRTIRATQPFGGSPAVSSLNP
                     GIVLPVYVCASGVNCATAGDRRLNPNNPFATAFAGDPANGAARIYYLFGDIPAGSDRS
                     NDVIRGTAGLKGSFGDGWDWRIEGVAARDNQRITQYGFLNIASRLKSINTGGYNFVNP
                     SANSEAVRDFVSPDVKTPSHSTLLSLDGSVTKSLWTLPGGDMQLALGGQIRKETLVNN
                     NQNERLDTYTLTTSSAFGEHTVMAGYFEVLAPVTEDLEINVSGRYDDYSEGFSHFSPK
                     IGAKYTPIRQLAFRGTYSKGFRAPTFAEAGARSQYAGFSTFTTPAAFQLAHGGLTSAG
                     NTNPYAQSYSLGGGYVGNPDLKPEKSRSFTAGVIFEPTRWLSATVDYYDVKKSDLIVA
                     GPLLANARNAYYTQTNVTDACAAVAAVGAGYSCNLIDAVDPLFPNALPRVLIINAPFV
                     NADYSKTSGVDVSATGRFDLPGEAKLTSRVEVTHV"
BASE COUNT          516 a          948 c          807 g          451 t
ORIGIN      
        1 gtcattttca aaatagtcat catgtcacct tctgaagaca agggagggcg ccgcgcatag
       61 atctgcaagt taggaatttg tcatgatacg gagcggacgt ttcacgattg caatagaccc
      121 aaccaatcaa tggaactcct tttgactata ttttgcagtc gaggggaact tctgtttttt
      181 cggtattcga tatcgtcttg ttgacgaaag aggggaattt atcgtgaagc aatacaagac
      241 tatacgacgg cgcctgttgg ggtcgagcat gatctgcggc gtggccatcg cggtgctggc
      301 cacaggccag gcctcggctc aaacggcggc gcccgagccc gagtcgacgg aaatcgaagc
      361 ggtggttgtg accggttcgc tgttccgccg caccgacacc gagaccccgt cgccggtcac
      421 cgtgatgacc agcgaaagcc tggcccgggc cggcatcacc acggccaccg acgccatccg
      481 ttcagtctcg gccgacggcg ccggctcgat cggcaccggc ttccagagcg gcttcagcgc
      541 cggcggctcg gccgtctcgc tgcgcggtct gggcgtgtcc tcgaccctgg tgctggtgga
      601 cggcctgcgc tcggccaact tcccgatcaa tgacgacggc cacaacgcct atgtcgacct
      661 gaactcgatc ccgttcagcc tgatcgaccg catcgaagtg ctgaaggacg gcgcctcgtc
      721 gagctatggc gccgacgcca tcggcggcgt ggtcaacctg atcctgaaga agcagttcgt
      781 cggcgtggcc ggcagcgccg aatttggcga aagcgatcgc ggcgacgccg agcacaggca
      841 gttcgacctg accctgggct atggcgacta tgcgcagagc ggctggaact tctacgtcaa
      901 cggcgagtac cagaaggacg gccgggtcac caaccacagc cgcggcttcc cgtacaacac
      961 ccaggacctg agccgcatcg gcggtctgga ccagaacacc gccgacagca ccctgaccac
     1021 ggccacgccc accgccgtcg tcacccgcgt cagccagacc gacctgaaca acccgctgtc
     1081 tggcatgatc ggcgccccgc tgaccaacca gtaccaggcc ctgaacctga actgcgccaa
     1141 cggctcctac acggtgacca cgggttcggc ccggggcacg agctgcaagt acgatctggt
     1201 cgatctctac aaccagatcg cgcccttcca ggagcgctac gccgtcaatg gtcgcctgag
     1261 cgtgcgcctg agcgacaaca tcgagggcta catcaccggc agcttctcgc atgacgaggt
     1321 gcggatcaaa aaccagccgc gcaccatccg ggccacccag ccgttcggcg gttcgcccgc
     1381 cgtctcgtcg ctgaacccgg gcatcgtgct gccggtctat gtctgcgcgt cgggcgtcaa
     1441 ctgcgccacg gccggcgacc gccgcctgaa ccccaacaac ccgttcgcca ccgccttcgc
     1501 gggcgatccg gccaacggcg cggcgcgcat ctactatctg tttggcgaca tcccggccgg
     1561 cagcgatcgc agcaatgacg tgatccgcgg cacagccggc ctcaagggca gcttcggcga
     1621 cggctgggac tggcgaatcg aaggggtcgc cgcacgcgac aaccagagga tcacccagta
     1681 cggcttcctc aacatcgcca gccggctgaa gtcgatcaac acgggcggct acaacttcgt
     1741 caatccgtcg gccaacagcg aagccgtccg cgacttcgtc tcgcccgatg tgaaaacccc
     1801 gtcgcactcg accctgctgt cgctggacgg ctcggtgacc aagtcgctgt ggaccctgcc
     1861 gggcggcgac atgcagctgg ccctgggcgg gcagatccgc aaggaaaccc tggtcaacaa
     1921 caaccagaac gaacgcctgg acacctatac cctgaccacc tcgtcggcct ttggcgagca
     1981 cacggtcatg gccggctatt tcgaagtgct ggcgccggtc accgaggacc tcgagatcaa
     2041 cgtctcgggt cgttatgacg actattcgga aggcttcagc cacttctcgc caaagatcgg
     2101 cgcgaaatat acgccgatcc gtcagctggc tttccggggc acctattcca agggtttccg
     2161 ggccccgacc ttcgccgaag ccggcgcacg cagccagtat gcgggcttct cgaccttcac
     2221 caccccggcc gccttccagc tggcgcacgg cggcctgacc tcggccggca acaccaaccc
     2281 ctacgcccag tcctactcgc tgggcggcgg ctatgtgggc aatccggacc tgaagccgga
     2341 gaagtcgcgc agcttcaccg ccggcgtgat cttcgagccc acccgctggc tgtcggccac
     2401 ggtcgactat tatgacgtca agaagtcgga cctgatcgtg gcgggaccgc tgctggccaa
     2461 cgcccgcaac gcctactaca cccagaccaa tgtgaccgac gcctgcgccg ccgtagcggc
     2521 cgtcggggcg ggctattcgt gcaacctgat cgacgccgtc gatccgctgt tcccgaacgc
     2581 cctgccgcgc gtgctgatca tcaacgcgcc gttcgtcaac gccgactact ccaagacctc
     2641 cggggtcgac gtctcggcca ccggccggtt cgacctgccg ggcgaggcca agctgaccag
     2701 ccgcgtcgag gtcacccacg tg
//
LOCUS       DAOMEO010000356         2713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4029_length_2713_cov_2.155756, whole genome shotgun sequence.
ACCESSION   DAOMEO010000356 DAOMEO010000000
VERSION     DAOMEO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_2713_cov_2.155756"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLH31_08880"
     CDS             complement(<1..716)
                     /locus_tag="PLH31_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HQR89359.1"
                     /translation="MPRATAQTRRNGKTSDTQSDLYTRVTGQILADLAQGVRPWTRPW
                     ASAHPAGSVRRPLRHNLIPYSGINTVLLWARAMAQGYGAPIWMTFRQAIELGGCVRRG
                     ERATTIVYANRLSRTEAGEDGHDVERSIPFLKAYGVFNIEQIDGLPESFAPAPVDPLP
                     QSARRDLLSGSGRRRRPCRRPGLVCAGSRSHPDAALRGVSIGRGLLRHPRSRMRPLDP
                     SSVPAGSRPGSQGGRRRRLCP"
     gene            complement(782..1099)
                     /locus_tag="PLH31_08885"
     CDS             complement(782..1099)
                     /locus_tag="PLH31_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HQR89360.1"
                     /translation="MATIGAFTANADGSYSGAIRTLSLNVKTVQFRPVEKTGERSPDF
                     RVYSGQTEFGAAWKKTSKDNNDYLSVKLDDPSFSEAMNAALVVIDGVHSLVWSRPSAA
                     KDD"
     assembly_gap    1771..1780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2026..2241
                     /locus_tag="PLH31_08890"
     CDS             2026..2241
                     /locus_tag="PLH31_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89361.1"
                     /translation="MIWVIYRHALALGGGVRRGETATPIAYTDRPAKTMIGGRPGRPE
                     IQSAPENGCDVLSEAMAIGAKRSRTAR"
     gene            2238..2639
                     /locus_tag="PLH31_08895"
     CDS             2238..2639
                     /locus_tag="PLH31_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQR89362.1"
                     /translation="MTALPLLAAAVIACTAPKVHDGDTLRCGAQRVRLFGVDAPELRR
                     GKTPAEPFAYEARDLLIDLTRGRVGCRIVNRDRYGRAVGRCWSSASPDLNAALIASGL
                     VTEYRRYSKGAYSAVQAEARNAKRGQWALRK"
BASE COUNT          423 a          905 c          951 g          424 t
ORIGIN      
        1 gggcatagcc ggcgtcgccg gccgccttgc gacccaggtc gcgatccagc cgggacggat
       61 gacgggtcca gtggacgcat tcgtgagcga gggtggcgta atagtcctcg cccgattgaa
      121 acgcctcgaa gggcggcatc tggatgcgat cgggacccgg cgcataccag gcctggtcgc
      181 ctgcatgggc gacgtcggcg cccagagcct gaaagaaggt ctcggcgggc gctttggggc
      241 aagggatcga ccggagccgg cgcgaaactt tccggcaggc cgtcgatctg ctcgatgttg
      301 aacacgccat aggctttgag gaacgggatc gatcgctcca cgtcgtgacc gtcctcgccg
      361 gcctcggttc tggacaggcg gttggcgtag acgatggtcg tcgcccgctc gccgcgtcgc
      421 acgcatccgc ccagttcgat cgcctggcgg aaggtcatcc agatcggcgc gccatagcct
      481 tgggccatgg cccgggccca gagcagcacc gtgttgatgc cgctataggg gatcaggttg
      541 tggcgcagcg gccggcggac actgccggcc ggatgggcgc tggcccaggg tcgggtccag
      601 gggcggacgc cctgggcgag atcggccagg atctgaccgg tgacgcgggt gtagagatcg
      661 ctttgggtgt cggacgtctt gccgttccga cgtgtttgag ccgtggcgcg gggcatcgcg
      721 gcctcctctt ggctgaaaat cggggaaggg gccgccccgc ctttcggcgg ggcggaaacg
      781 gtcagtcgtc cttggcggcg ctgggccgcg accagaccag gctgtggacg ccgtcgatga
      841 cgaccagggc ggcgttcatc gcttcggaga agctggggtc gtccagcttg accgacaggt
      901 agtcgttgtt gtccttgctg gtcttcttcc aggccgcgcc aaattcggtc tggccggaat
      961 agacccggaa gtccgggctg cgctcgccgg tcttctcgac cggacggaac tggaccgtct
     1021 tgacgttaag gctgagggtg cggatcgcgc ccgagtaaga accgtccgcg ttggcggtga
     1081 aggcgccgat ggtggccatg gtggtctctc cgatctaagg ccgggccgcg ccgatcgcgg
     1141 cctcgatggc gatcgaaaga ccggggccga cgtccggacc gcagcgcgcc ccagcccgtc
     1201 aaagggtcgg cacgacgcac gggatgacgg gggcgcgccc gcagcgcagc gcaggaccga
     1261 aggcggcaag tttcttgtct cgcgaggaat ggccaaggcc cgaagggccg ccggccaggg
     1321 gaagaaaggc gccggcgagg ttgcgggaac gaccggtttc cggtcagatc aggccagaag
     1381 aggacgcggt cgacgtggcc cggctcggat catcagagac tcgccttggc catctcggcg
     1441 tcttcaccgc cagcgcgggc ggcggggctc taacgcgcgc gcctccgcgc cctcagccgc
     1501 atcgtcaaga cggtccagtt ccgccccctc gatgagacag gcgacctcgt cccgacctcc
     1561 gaagctattt cggccgaccc cattttggcg cggtcttgag acggcgagcc aggacatcaa
     1621 agcgatccgt cggtgggctg acggtcccgt ttctcggagg cgttaaaccc cgccccggtc
     1681 gtcatcgacg gcgtccacag cctggtctgg tcgcgacccg ccaccgccaa ggacggctga
     1741 ccgtcgccgc ccgccgtgcg gcggagcggt nnnnnnnnnn cgttgccgat ttcagtccga
     1801 tgaaaacgcc gacgccccgt ccaagtgaga cgcccggatc ggctgtcccg cgctcgattt
     1861 caccgcctcc ccaaccagat ctcgccgacc tcgccgaggg cgccagcccg gcccttgcca
     1921 agccggccac cggcttgtcc atcaccttcc cccgcgacgt cgccagagcc ttgggcatcg
     1981 agccgtcgct ggcggctagg cgcgacagag gctggctatg gcgcgttgat ttgggtgatc
     2041 tatcgccacg cccttgcgct gggcggtggc gtgcggcgcg gcgagacagc gacgcccatc
     2101 gcctataccg accgtcccgc caagaccatg atcggagggc ggccaggacg cccggagatc
     2161 caatctgctc ctgaaaacgg ttgcgacgtt ttgagcgagg cgatggcgat cggcgcgaag
     2221 cgatctagaa cggcgcgatg accgccctgc ccctgcttgc cgccgccgtg atcgcctgca
     2281 ccgcccccaa ggtgcatgac ggcgacaccc tgcgctgcgg ggcgcagcgg gtccggctgt
     2341 tcggggtcga tgcgcctgag ctgcgacgcg gtaagacgcc cgccgagccc ttcgcctatg
     2401 aagcccgcga cctgctgatc gacctgacgc gcggccgggt cggttgccgg atcgtcaacc
     2461 gcgaccgcta cggccgcgcc gtgggccgct gctggtcgag cgcctcgccc gatctcaacg
     2521 cggccctgat cgcctcgggc ctggtcaccg agtatcgccg ctacagcaag ggcgcctata
     2581 gcgctgtgca agccgaggcc cggaacgcca agcggggcca gtgggcgtta aggaaatagc
     2641 ggagccgagc gcgcacgaaa gcggccgatc ccaaaggatc ggccgccccg ctctcgcggt
     2701 ctcacgaaac ttg
//
LOCUS       DAOMEO010000357         2702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4050_length_2702_cov_2.807707, whole genome shotgun sequence.
ACCESSION   DAOMEO010000357 DAOMEO010000000
VERSION     DAOMEO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_2702_cov_2.807707"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLH31_08900"
     CDS             <1..556
                     /locus_tag="PLH31_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR89363.1"
                     /translation="VKDSQRLGEVGEGWKEALVTLMNERLAVGGSSGPNYREIMGLAR
                     ELTGATGPAIKDNAFREKLADWYVQSEGLKFTRFRTMTALSRGQTPGPESSIGKIIAA
                     NQMQDLANTAVEMEDQYGILIDPDQAPAEAAFQQSLMWAPGLRIAGGTDEILKNIIAE
                     RVLGLPGDVRVDKDVAFKDMPTGR"
     gene            851..2362
                     /locus_tag="PLH31_08905"
     CDS             851..2362
                     /locus_tag="PLH31_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89364.1"
                     /translation="MHEVSDAVSRYSALRKTLLGAVSGRGAFLRRVPVGALVAAVVIP
                     LVGCDVGPNLWFQKRASACPRPQAAQGVSGQFNQQQQEIRALRQGGFRGDFFAQLELA
                     RRYEGQRAVDKNLEDQVEAATWYAMALANAGGYAPIAAYERRGKNDGRTLSRFDDCRA
                     FERQSAYGALDRLLSRMSTEEREKVRNRVIYVLSSQGADGYRVLARMHDGFFGPYGEP
                     SDNLQAVEAYGGPKRAGAPAALDLFRRNDVDAYLYNYLAVQTGDVSAYVMLKDFERSS
                     PQRASYGAFVETKAKRWIPPYEFYPPESPESGVPHSDEASQSGEAKEAALTRLHELPF
                     VHIGEALAYLRVIPTPLIDEKLLSTNDAQTFQAMIGRPISGRLSAIEKARAVQYAAVN
                     GSSKAQLVLAVMYSEGVGVPRDYARAYHWYEEAERQGSAEAKYAMSTFFSLGLQGVAD
                     QDRAKAVVYQLDGALAGFKPSVWRLQQLLAQVSRPPRPAAERGGRPYIERDYR"
     gene            2359..>2702
                     /locus_tag="PLH31_08910"
     CDS             2359..>2702
                     /locus_tag="PLH31_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89365.1"
                     /translation="MSRLSRFLALVATLSLGLAAAPAVALASPDAKIDQQIRPCFGLL
                     LEPNLSRGCKTSHRPKVKPCPRGSYRVRGRCEYGNGGRPGYVPREINCDYAYPGQVND
                     TLARLYDGDLLTL"
BASE COUNT          448 a          882 c          890 g          482 t
ORIGIN      
        1 cgtcaaggac agccagcggc tgggcgaggt cggcgagggc tggaaggagg ccctggtcac
       61 cctgatgaac gagcgtctgg cggtcggcgg ctcatcgggc cccaactatc gcgagatcat
      121 gggcctggcc cgcgagctga ccggcgccac cggcccggcg atcaaggaca acgccttccg
      181 cgaaaagctg gccgactggt atgtgcagtc cgagggcctg aagttcaccc gcttccgcac
      241 catgaccgcc ctgtcacgcg gccagacgcc gggaccggaa agctcgatcg gcaaaatcat
      301 cgccgccaac cagatgcagg acctggccaa caccgccgtc gagatggaag accagtacgg
      361 tatcctgatc gatcctgatc aggccccggc cgaggcggcg ttccagcaga gcctgatgtg
      421 ggcccccggg cttcgtattg ccggcggcac cgacgagatc ctcaagaaca tcatcgccga
      481 gcgggttctg ggcttgccgg gtgatgtgcg ggtcgacaag gacgtggcct tcaaggacat
      541 gccgacgggg cgttagggcg aactggcgcg actggcttca aatcgcgcac accgcgctca
      601 tgagacgagc gcgccgagga attaatccgc gaggagacga gttctcctcg cggttgcatt
      661 tgtcacttgt tcgataagtg ttgcctcggc tgttttgtga catttccgac agattctcct
      721 gccgcgcgat ttgttgcgca gcgcgaaaac acgccggtgg tcgaacggcg ttgcttcacc
      781 ttgacagtat gcaaccccga gcacatgagt cctcgatgct tatggttaac ggcgtgctgg
      841 agggggagtg atgcacgaag tcagcgacgc tgtttcgcgc tattccgcgc tccgaaagac
      901 cctgcttggg gcagtttccg ggcgaggggc gtttctgagg cgtgttcccg tcggagcgct
      961 ggtcgccgcc gtggtgattc cccttgtggg ttgcgacgtg ggtcccaatc tctggttcca
     1021 gaagcgcgcc agcgcctgtc cgcggccgca ggcggcccag ggggtgtcgg gccagttcaa
     1081 ccagcaacag caggagatcc gggcgctgcg ccagggcggt ttccggggcg acttcttcgc
     1141 tcagctggaa ctggcgcggc ggtatgaagg ccagcgggcc gtcgacaaga atctcgagga
     1201 tcaggtcgag gccgccacct ggtacgccat ggccctggcc aatgctggcg gctatgcgcc
     1261 gattgcagcc tatgagcggc gcggcaagaa tgacggcagg accctgtcgc gcttcgatga
     1321 ctgccgcgct tttgagcgtc agagcgccta tggcgccctc gatcgcctcc tgtcgcgcat
     1381 gtcgaccgag gagcgcgaga aggtccgcaa tcgcgtgatc tatgtgctgt ccagccaggg
     1441 cgccgatggc tatcgcgtgt tggcccgcat gcatgacggc ttcttcggcc cgtatggcga
     1501 gccgtctgac aatcttcagg ccgtcgaggc ctatggcggt cccaagcgcg ctggcgcgcc
     1561 ggcggcgctg gatctgttcc gtcgcaacga cgtcgacgcc tatctctaca attatctggc
     1621 ggtgcagacg ggcgacgtct cggcctatgt gatgctcaag gacttcgagc gctcttcgcc
     1681 gcaacgcgcc tcttacgggg cgtttgtcga gaccaaggcc aagcgctgga ttccgcccta
     1741 cgagttctat ccgcccgaat cgccggagtc gggcgtgccg cattccgacg aagccagcca
     1801 gtccggcgag gccaaggaag cagccctgac ccgtctgcat gagctgccct tcgtgcatat
     1861 cggcgaggct cttgcctatc tgcgcgtgat ccccacgccg ctgatcgacg agaaactgct
     1921 ctcgaccaat gacgcccaga ccttccaggc catgatcggc cggccgatca gtgggcggct
     1981 gagcgccatc gagaaggcgc gggcggtgca gtacgccgcc gtcaacggct cgtccaaggc
     2041 ccagctcgtt ctggcggtga tgtattccga gggtgtgggc gtgccgcgcg actacgcccg
     2101 ggcctaccac tggtacgagg aagccgaacg tcagggctcg gctgaggcca agtacgccat
     2161 gtcgaccttc ttctcgctgg gccttcaggg cgtggcggac caggatcgcg ccaaggcggt
     2221 ggtctaccag ctggacggcg ccctggccgg cttcaagccg tcggtctggc gcctgcaaca
     2281 attgctggcg caggtttcgc gtccgcccag acccgccgcc gagcgcggcg gtcggcccta
     2341 catcgaaagg gactatcgat gagccgcctg agccgcttcc tggccctcgt ggccacgctg
     2401 tcgctcggcc tcgccgccgc gcccgccgtg gcgttggcct cgccggacgc caagatcgat
     2461 cagcagatcc gtccgtgttt cggccttctg ctcgagccga acctgtcgcg aggctgcaag
     2521 acgagccacc ggcccaaggt caagccctgt ccgcgcggca gctatcgcgt tcgcggccgc
     2581 tgcgaatacg gcaatggcgg tcgccccggc tatgtccccc gcgagatcaa ctgcgactat
     2641 gcctatcccg ggcaggtcaa tgacaccctg gcccgcctgt acgacggcga cctgctgacc
     2701 ct
//
LOCUS       DAOMEO010000358         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4066_length_2696_cov_2.226808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000358 DAOMEO010000000
VERSION     DAOMEO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_2696_cov_2.226808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>954)
                     /locus_tag="PLH31_08915"
     CDS             complement(<1..>954)
                     /locus_tag="PLH31_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HQR89366.1"
                     /translation="GLTAVRRLETWTAPIKLVICALMVWWAMDKAGGLGPIFHQPSAF
                     VDGGPKAGQFWSVFVPALTAMVGFWATLALNIPDFTRFAKKQADQILGQAIGLPIPMA
                     LLALVSVITTSATVIVFGKAVWDPVQLAGDIGGIAVVIGLLIISLDTVCCNIAANLVG
                     PAYDFSALWPSKITYRLGGWITAGIGVLIMPWKLLETTEGYIFVWLVGYGALLGPIAG
                     ILIVDYWLLRKTRLAVADLYEKGGAYAYRGGWNPAALAALAVGVAPNLPGFLASAAPH
                     LFGGVGAFWTGLYTYAWFVGLALAGGIYWGMMRSSLARTGD"
     assembly_gap    955..964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<965..1468)
                     /locus_tag="PLH31_08920"
     CDS             complement(<965..1468)
                     /locus_tag="PLH31_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HQR89367.1"
                     /translation="MSAPDSDLWNEDLAPTGEAQRTWRWWHYAALWLGMVICVPAYML
                     ASGLIEQGMSAGQAVATVLLGNLIVLVPMLLIGHAGARHGVPYAVLARASFGWKGARI
                     PAVARAVVACGWYGIQTWIGGGALLTLLGVIMGRSLVGAPIPLLGIGVGQLLAFLAFW
                     GIQLLFVT"
     gene            complement(1606..1797)
                     /locus_tag="PLH31_08925"
     CDS             complement(1606..1797)
                     /locus_tag="PLH31_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89368.1"
                     /translation="MARKSYATGETQSVFFRACDITKPAPPVLRHGGPCFDWGRLALV
                     SLFFVLAVGGATLAYAGLS"
     gene            complement(1971..2405)
                     /locus_tag="PLH31_08930"
     CDS             complement(1971..2405)
                     /locus_tag="PLH31_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HQR89369.1"
                     /translation="MSKTTPPLRLLAQDAEDLAVLSAALQDAVAKIGDIHWDAQARTL
                     TLACNRFRWEAGGKTGERVRSALQLGDVTGVQARNLKRDAKGAIVELLSISFEPGEAP
                     GGTVLLTFAGGGDLRVGVDCLDVVLADVSEPWATPRRPGHAD"
     gene            complement(2406..>2696)
                     /locus_tag="PLH31_08935"
     CDS             complement(2406..>2696)
                     /locus_tag="PLH31_08935"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517808.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQR89370.1"
                     /translation="ENRFMHAPELMRLGADISVSGGEARVRGVAQLEGAEVMATDLRA
                     SVSLVIAGLAARGETVVSRIYHLDRGFERLEEKLTACGAQVRRIKGDGEAEL"
BASE COUNT          499 a          955 c          872 g          360 t
ORIGIN      
        1 caatcgccgg ttctggccag cgacgaccgc atcatgcccc aatagatccc gcccgccagc
       61 gccagcccca cgaaccacgc atacgtataa agcccggtcc agaacgcccc aacaccgcca
      121 aacaggtgcg gcgcggccga ggccaggaag ccgggcagat tgggcgccac gccgaccgcc
      181 agggctgcca gcgccgccgg gttccagccg ccgcgatagg cataggcccc gcccttctcg
      241 tagagatcgg ccacggccag ccgggtcttc cgcagcagcc agtaatccac gatcaggatg
      301 cccgcgatcg gtcccagcag cgcgccatag ccgaccagcc agacgaagat gtagccttca
      361 gtggtctcca gcagcttcca cggcatgatc agcacgccga tcccggccgt gatccagccg
      421 ccgaggcgat aggtgatctt gctgggccac agggcggaaa agtcataggc cgggccgacc
      481 agattggcgg cgatgttgca gcacaccgtg tcgaggctga tgatcagcag gccgatcacc
      541 accgcgatgc cgccgatgtc gccggccagc tgcaccggat cccacacagc cttgccaaag
      601 acgatcaccg tggccgaggt agtgatcacg ctgaccaggg ccagcagggc catcgggatc
      661 ggcaggccga tggcctggcc caggatctgg tcggcctgtt tcttcgcaaa gcgggtgaag
      721 tcgggaatgt tcagggccaa ggtcgcccag aacccgacca tggccgtcag ggcgggcacg
      781 aagacactcc agaactggcc ggccttgggg ccgccgtcga cgaaggccga gggctgatgg
      841 aagatcggac ccagacctcc ggccttgtcc atcgcccacc acaccatcag ggcgcagatg
      901 accagcttga tcggcgcggt ccaggtctcc agccggcgca cggcggtcag gcccnnnnnn
      961 nnnnggtgac gaacagcagc tggatgcccc agaaggccag gaacgccagc agctggccca
     1021 cgccgatgcc cagcagcggg atcggcgcgc ccaccaggct gcgccccatg atcacgccca
     1081 gcagggtcag cagcgcgccg ccgccgatcc aggtctggat gccgtaccag ccgcaggcca
     1141 ccacggcccg ggcgacggcg ggaatccgcg cgcccttcca gccgaacgag gctcgcgcca
     1201 gcaccgcata gggcacgccg tgcctggccc ccgcatggcc gatcagcagc atgggaacca
     1261 gaacgatcag attgcccagc agcacagtgg ccaccgcctg cccggccgac atgccctgct
     1321 cgatcaggcc cgaggcgagc atgtaggcgg gcacgcagat gaccatgcca agccacaggg
     1381 ccgcgtagtg ccaccagcgc caggtgcgct gcgcctcgcc cgttggcgcc aggtcttcgt
     1441 tccagagatc gctatcgggg gcgctcatgc gtcggtttcc cgagggcgca tggcgtcagg
     1501 tcgcgccggc ccccgcctcg cacgcgatgg acaagcttag ggggcgccgg gggaaactga
     1561 caatcggcgg aacgacgccc ggccgctccg ccaatttcgg atagcctaac tcagccccgc
     1621 ataggccagg gtcgccccgc cgacggcgag aacgaagaac aggctcacca gagccagacg
     1681 cccccagtcg aaacagggac cgccatgtcg caagacgggc ggagcaggtt tggtgatgtc
     1741 gcacgcacgg aaaaacaccg actgggtttc cccggtcgca taggacttcc tggccatgac
     1801 ggcctcctga cgatgctcgc gtctctgacg ggcgcgtggc gttaacctag caccgttccc
     1861 gcctcgggcg agttcacccg cgatcactag cgcgtgggac gaatcctcgg cgccgcgact
     1921 gctcgtttgg aagtcagttt aaaggcatgc ccgcaaatcg agcagggcgc ctagtcggcg
     1981 tgccccggcc ggcgcggcgt ggcccagggc tcggacacat cggccagcac cacgtccagg
     2041 caatcgacgc caacccgcag gtcgccgccg ccggcgaagg tcagcagcac agtgccgccc
     2101 ggcgcctcgc cgggctcgaa gctgatcgac agcagttcga caatggcccc cttggcgtcg
     2161 cgtttgaggt tgcgggcctg gacgccggtg acatcgccca gttgcagggc cgagcgcacg
     2221 cgctcacccg ttttgccgcc agcctcccag cggaagcgat tacaggccag ggtcagggtg
     2281 cgggcctgag catcccaatg gatgtcgcca atcttggcca cagcgtcctg aagggcggca
     2341 gacagcaccg ccaggtcctc ggcgtcctgg gccagcagtc gcagaggggg tgtggtcttt
     2401 gacatctaga gttcggcctc tccgtcgccc ttgatccgac gcacctgagc cccgcaggcg
     2461 gtgagctttt cttccaaacg ctcgaaaccg cgatccaggt gatagatgcg cgacaccacc
     2521 gtctcgccgc gggcggccag accggcgatc accaggctga ccgaggcgcg cagatcggtg
     2581 gccatcacct cggcgccttc cagctgggcg acgccgcgca cgcgggcctc gccgccagat
     2641 accgagatgt cggcgcccag acgcatcagc tcgggggcgt gcatgaagcg gttctc
//
LOCUS       DAOMEO010000359         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4068_length_2693_cov_2.822593, whole genome shotgun sequence.
ACCESSION   DAOMEO010000359 DAOMEO010000000
VERSION     DAOMEO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_2693_cov_2.822593"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..937
                     /locus_tag="PLH31_08940"
     CDS             164..937
                     /locus_tag="PLH31_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR89371.1"
                     /translation="MRLSTLALAALLSVTALTAHAQTPADPLHVYGPGGPAPAMREAA
                     KAYEARTGRAVAVVAGPTPQWLDQAKADGDLIFSGSETMMTDFVSALEGRIRAEAVEP
                     LYLRTSSILVRPGNPNGIGGLEDLFQPGRRVLVVNGAGQNGLWEDMAGRTGDIAKVRA
                     LRRNIVVYARNSAEARQAWIDDPTLDAWIIWGIWHVANPDLADVVEVEEPYRIYRDAG
                     AVVTETGRQDADAQPFLEFLKSDEGAAIFARWGWRTGQP"
     gene            973..>2434
                     /locus_tag="PLH31_08945"
     CDS             973..>2434
                     /locus_tag="PLH31_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR89372.1"
                     /translation="MANRRSFTLNRRHLLAAAAAPLWLSTTARAVSAPAALNITALTA
                     AFYAARGGRPAWAGPARRQAMDALALADRHGLSAPDGLALGEAELTQAILTLAEALAH
                     GRVDPASVETLWEMGRNQVATPPLLATAVDDGTLAETMAGLAPQDRGYQGLCDGFLRY
                     RAIADRGGWPPFALGATIEPFASDPRLPALLPRLRVEGDLSDAAAAMVGPSGGDYGSV
                     LQEAVRAFQVRHGLEADARIGPATQRALSISAEARARQIALNLERRRWLKRDVAPERI
                     EVNTAASIMVYWKDDRPVHSMRVVTGDADNATPSLERPFASVVANPPWTVPTSIAQRE
                     ILPRGAAYMRANNMTIQNGMVVQRAGPNAALGQVKFELQDSYAIFLHDTPSRGAFDQS
                     FRHLSHGCVRVQDAVGFARLLLAPDPERLARFDAALDSGQTVRVATGRPIDVRLLYWT
                     AFLDGQGRVAFRDDIYRRDTRLAEALGIALHLPRPDD"
     assembly_gap    2435..2459
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2460..>2693)
                     /locus_tag="PLH31_08950"
     CDS             complement(<2460..>2693)
                     /locus_tag="PLH31_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR89373.1"
                     /translation="PPGQEIGEEVHDDRDQFFRIEAGEGEIWIDGVRHAVKADDGVIV
                     PQSARHNVVSTGSEPLRLYTLYGPPEHLDGTVHA"
BASE COUNT          388 a          988 c          875 g          417 t
ORIGIN      
        1 ggcttcgcga cggccgggat tccaccctgc ccctgccgaa cctgatgctc tacgcgcttc
       61 aggtgaacat tcgcggcggc gtgttgccgc cgcccgcgcc ggatgggcgg gtatatttca
      121 agatccccgc caatcacttc gccccgcccc aggagaactc ttgatgcgtc tgtccacact
      181 ggcgttggcc gccctattga gcgtaacggc cctgaccgcc cacgcccaga cgcccgccga
      241 tcctcttcat gtctacggtc ccggcggccc cgcccccgcc atgcgcgaag cggcgaaggc
      301 ctatgaggcg cgcaccggtc gcgctgtggc cgtggtcgcc ggacccacac cccaatggct
      361 ggaccaggcc aaggcggacg gcgacctgat cttctccggc tccgagacga tgatgaccga
      421 cttcgtcagc gctctggagg gccgcatccg cgccgaggcg gtcgagccgc tctatctgcg
      481 aacgtcctct atcctcgtgc ggccgggaaa tccgaacggc atcggcggcc tggaagatct
      541 cttccagccg ggtcgccgag tcctggtcgt caacggcgcg ggccagaacg gcctgtggga
      601 ggacatggcc ggtcgcaccg gcgacatcgc caaggtgcgc gccctgcgtc gcaacatcgt
      661 cgtctacgcc cgcaacagcg ccgaggcccg acaggcctgg atcgacgacc cgacgctgga
      721 cgcctggatc atctggggca tctggcacgt cgccaatccg gacctcgctg atgtggtcga
      781 ggtcgaagag ccctaccgca tctaccgcga cgctggcgcc gttgtgaccg agaccggccg
      841 ccaggacgcc gacgcccagc cgttccttga atttctgaag tccgatgagg gggccgccat
      901 cttcgcgcgt tggggctggc ggaccggcca accttaagcc tgccttaagg tgaaccccgc
      961 ggtgtcggcg gcatggccaa ccgtcgctcc ttcaccctga accgccgaca cctgctcgcg
     1021 gcggccgcgg cgccgctctg gctctcgacg accgcgcgag ccgtatcggc gcccgccgcg
     1081 ttgaacatca ccgccctgac cgcagccttc tacgccgccc gcggcggacg cccggcctgg
     1141 gcggggccag cgcgccggca agccatggac gccttggccc tggcggaccg ccacggccta
     1201 tcagcgcccg acggcctcgc cctgggcgaa gccgagctga cacaggccat cctgaccctg
     1261 gccgaggcgc tcgcgcatgg ccgggtcgat ccagcatcgg tggagacgct ttgggagatg
     1321 ggtcgcaacc aggtcgctac gccgccgctg ctggcgacgg cggtcgacga cggaacgttg
     1381 gccgagacga tggcgggcct cgcgccccag gaccgcggct atcagggcct gtgcgacggc
     1441 ttcctccgct atcgcgccat cgccgaccgc ggcggttggc cgcccttcgc cctgggcgca
     1501 acgatcgaac cgtttgcgtc cgacccccgc cttcccgccc tcctgccgcg ccttcgggtg
     1561 gaaggcgatc tgagcgacgc cgccgccgcg atggtcggcc cgtctggcgg tgactacggc
     1621 tccgttctgc aagaggccgt tcgggcgttc caggtgcgcc acggtctgga agcggacgcc
     1681 cgcatcggcc cggcgaccca gcgggcgctg tcgatttcgg ccgaggcccg tgctcgccag
     1741 atcgcgctca atctggaacg ccgccgttgg ctgaagcggg acgtggcccc ggaacggatc
     1801 gaggtgaaca ccgccgcctc catcatggtc tactggaagg atgatcggcc cgtgcattcc
     1861 atgcgggtcg tgacgggaga cgcggacaat gcgacgccca gcctcgaacg gcccttcgcc
     1921 tcggtcgtcg ctaatccgcc ctggacggtc ccgacctcca tcgcccagcg cgaaatcctg
     1981 ccgcgcggcg ccgcctatat gcgggcgaac aacatgacga tccagaacgg catggtggtt
     2041 cagcgcgccg gcccgaacgc cgccctgggc caggtcaagt tcgagcttca ggacagctac
     2101 gccatcttcc tgcacgacac cccgtcgcgg ggtgcgttcg accagagctt ccgccatctc
     2161 agccacggct gcgtgcgggt gcaggacgcc gtcggcttcg cccgacttct gctggcgccc
     2221 gaccccgaaa ggctggcccg cttcgacgcc gcgctggaca gcggccagac cgtccgggtg
     2281 gcgaccggtc ggccgatcga cgtgcgcctg ctgtattgga ccgccttcct ggacggccag
     2341 ggccgcgtcg cctttcgcga tgacatctat cgtcgcgaca cccgtctggc ggaggcgttg
     2401 ggcatcgccc tgcacctgcc gcggcccgac gaccnnnnnn nnnnnnnnnn nnnnnnnnng
     2461 gcgtgaaccg tcccgtcgag atgctccggc ggaccataga gggtgtagag ccgaagcggc
     2521 tcggaccccg tgctgaccac attgtgccgg gcgctctgag ggacgatgac cccgtcgtcc
     2581 gccttcacgg cgtgacgaac gccgtcgatc cagatttcgc cctcgcccgc ctcgatgcgg
     2641 aagaactggt cgcggtcgtc gtggacctct tcgccgatct cctgaccggg cgg
//
LOCUS       DAOMEO010000360         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4073_length_2692_cov_2.723929, whole genome shotgun sequence.
ACCESSION   DAOMEO010000360 DAOMEO010000000
VERSION     DAOMEO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4073_length_2692_cov_2.723929"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLH31_08955"
     CDS             <1..238
                     /locus_tag="PLH31_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89374.1"
                     /translation="QDRASGAVGVVLELNDGSHADDIVGHVAVGPDFCTVDLLEQQAG
                     GEGRGMLHLQGEGRKLFGFSISDDNVRARESFCR"
     gene            complement(475..2274)
                     /locus_tag="PLH31_08960"
     CDS             complement(475..2274)
                     /locus_tag="PLH31_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR89375.1"
                     /translation="MFAERRPSDPSTRGANLAMALDAQTALLPYALGVFAISLPIFTW
                     VGSFADDAFWMLSSFVVFAINWGAFYAAVSWLRGEGSQDLKRRARVQLLGGLLWAVAV
                     IQLSAFANAAGPVRETLLLLATAAAVLCIFFSSTSLPALLLTAPVAAVGPLVALFSQP
                     ESRHAGVMAWGAIALTLAMALVLNRMIRRQHALAEAHERLIQERLAVLDAAERTARSK
                     SDIVATLSHEIRNGLTGVTHVMAAAAGKGGRAAPSREQLNAALDAAQDLIAVLNATLD
                     SETAESGRLAVEALPFEPVRLLRDLALLERPHALAKGLELAVHVDPLLDRRTRGAVLA
                     DAMRTRQIIANLLGNAVKYTVRGRIEVRIELRGDVVAIEVADTGPGLTSEELEHAFEP
                     FRRVERTGAGVNGAGLGLSLSRQLARLMGGALDARSAVGVGSCFTLTLPFDQTLTCIT
                     ETAEEAAQAAEAPGAPRSLRVLIAEDDALNAAMLRAILEQLGHQVVHAQNGQRAADLA
                     RIAEFDLLMLDGRMPVLDGPGAAAAVRALDGKNRDMPIIAVIDGDADEARDCMDAGAD
                     IVLRKPVSVAGVARAVADASALERLTVAGRAAA"
     gene            2434..>2692
                     /locus_tag="PLH31_08965"
     CDS             2434..>2692
                     /locus_tag="PLH31_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89376.1"
                     /translation="MATTRAALTDNDIRMLVKGATADERALAAHKLCRTIDRADLADD
                     ERQIAHDILRVMATDAAELVRRAMAVTLKNSPSLPPDVANRL"
BASE COUNT          473 a          843 c          967 g          409 t
ORIGIN      
        1 gcaagaccgt gcgtctggcg ccgtgggcgt cgtcctcgaa ctgaacgatg gatcgcatgc
       61 ggacgacatt gtcgggcatg tcgccgtcgg gccggatttc tgcacggtcg atctcctcga
      121 gcagcaggcc ggcggcgagg ggcgcggcat gctccatttg cagggcgagg gccgaaagct
      181 gttcggcttc agtatcagcg atgataatgt gcgggcgcgc gaaagcttct gccggtaaag
      241 gcatggagga ctcctggatt tgacatgaaa agacgcgccg tttgaccggc aggcatcaat
      301 gtaaatgaga tggaagtgtc gccccgaacc cctgcgccgt cggcgcggat tgtccggggc
      361 tagaggcctg cttgcaggct accgccgtgg tggcgaaaac gccggaatgt cgatcgcacg
      421 aacatgggga tcattgatcc acctgtcggg ccgccttgtc aaatcttgaa gccttcaggc
      481 ggcggctcgc ccggccacgg tcaagcgctc caacgccgag gcgtcggcga ccgcgcgggc
      541 gacgccggcc acggagaccg gcttgcgcag gacgatgtcg gcgccggcgt ccatgcagtc
      601 gcgagcctcg tcagcgtcgc cgtcgatcac ggcgatgatc ggcatgtcgc ggttcttgcc
      661 atccagggcg cggacggcgg ccgcggcgcc cggaccgtcg agcaccggca tgcggccgtc
      721 cagcatcagc aggtcgaatt cggcgatccg ggcgaggtca gcggcccgct ggccgttctg
      781 ggcgtggacc acctggtggc ccagctgctc gagaatcgcg cgcagcatgg cggcgttcaa
      841 cgcgtcgtcc tcggcgatca acacgcgcag gctacggggc gcgccggggg cttcggcggc
      901 ctgagcagcc tcttcggcgg tttcggttat gcaggtcagg gtctggtcaa agggcagggt
      961 aagggtgaag cagctgccga cgccgacggc gctgcgggcg tcaagggcgc cgcccatcag
     1021 gcgggccagc tgacgcgaca gggacaggcc aaggcctgcg ccattgaccc ccgcgccggt
     1081 gcgctcgaca cggcggaagg gctcgaacgc gtgttccagt tcctcggacg tgaggccagg
     1141 accggtatcg gcgacctcta tggctaccac gtcgccgcgc agctcgatgc gaacctcgat
     1201 gcggcccctt acggtgtact tgacggcatt gcccagcaag ttggcgatga tctgacgtgt
     1261 ccgcatggcg tcggccagaa ccgcgccgcg cgtgcggcga tccaggagcg gatcgacatg
     1321 gacagccagt tccaggccct tggccagggc gtggggacgt tccagcaggg ccaggtcccg
     1381 cagcagacgg accggctcga agggcagggc ctcgacagcc aggcgaccgg actcggcggt
     1441 ctcggagtcg agggtcgcgt tcaacaccgc gatcaggtcc tgggcggcgt cgagggcggc
     1501 gttcagctgt tcgcgcgacg gggcggcgcg gccgcccttg cctgcggcgg cggccatgac
     1561 gtgggttacg ccggtcaagc cattgcggat ttcgtggctc agggtggcga cgatgtcgga
     1621 cttggagcgc gccgtgcgtt ccgcggcgtc gaggacggcg aggcgttctt ggatcaggcg
     1681 ctcgtgcgcc tcggccagcg catgctgccg gcggatcatg cggttcagga ccagcgccat
     1741 ggccagggtc aaggcgatgg cgccccaggc catgacgccg gcatggcggc tttcgggctg
     1801 cgagaacagg gcgaccaagg ggccgacggc ggcgacaggc gcggtcagga gcagggctgg
     1861 aagcgatgtc gaggaaaaga aaatgcacag caccgctgca gccgtggcca gcagaagcaa
     1921 tgtctcccgg accgggccgg cggcgttggc gaaggccgac agctggatca cggctacggc
     1981 ccagagcagg ccgcccagga gctgcacgcg agcccgacgt ttaagatctt gcgacccttc
     2041 gccgcgcagc cagctgaccg ccgcatagaa ggcgccccag ttgatggcga aaacgacgaa
     2101 gctggaaagc atccagaagg cgtcgtcggc gaacgatccg acccaggtga aaatgggcag
     2161 gctgatcgcg aaaaccccaa gcgcgtaagg caacagcgcg gtttgggcgt caagcgccat
     2221 ggcgaggttt gcgccgcgcg tcgagggatc agaagggcgg cgctcggcga acaaggatcg
     2281 cattcctcgg ctgttgagcg cggagcctag gcgatcacgg tttgcggctg gttacggggc
     2341 atcgttaatc cacaggtttc agttcaaaca atcattaagc ctaacaggac atggtagagc
     2401 tgatctgcat ccctctggtt cggcgaacct tccatggcca ccacccgcgc ggcactgacc
     2461 gacaacgata tccggatgct cgtgaagggc gccacggctg acgagcgcgc gctcgctgcc
     2521 cataagctgt gccgcaccat cgaccgcgcc gacctggccg atgacgagcg ccagatcgct
     2581 cacgacattc tgcgggtgat ggcgacggac gccgccgagc tggtgcgtcg ggccatggcc
     2641 gtgacgctga agaactcgcc cagcctgccg ccggacgtcg ccaaccgtct gg
//
LOCUS       DAOMEO010000361         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4080_length_2689_cov_2.036826, whole genome shotgun sequence.
ACCESSION   DAOMEO010000361 DAOMEO010000000
VERSION     DAOMEO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4080_length_2689_cov_2.036826"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>235
                     /locus_tag="PLH31_08970"
     CDS             <1..>235
                     /locus_tag="PLH31_08970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HQR89377.1"
                     /translation="GWKPGVGWAEEVILPPGFDYGLSEGPRELPSWWEARGVRRADGL
                     PWSAADAASPAGLILPAGASGPAFLALPNHFAIR"
     assembly_gap    236..245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <246..529
                     /locus_tag="PLH31_08975"
     CDS             <246..529
                     /locus_tag="PLH31_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HQR89378.1"
                     /translation="SYALGIGLLADRFGGGDPIIGAWPVDAPLSLSDRMAAQIALARL
                     GFDPGAPDGVVGAGTRKALRAWQQSRGLPADGYMSMDMVQRLKAQAGLS"
     gene            complement(577..1692)
                     /locus_tag="PLH31_08980"
     CDS             complement(577..1692)
                     /locus_tag="PLH31_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR89379.1"
                     /translation="MMTLATLPPVTALAALAPVTDLGAMPGATPSGSVAPPGCAPTAE
                     ISVLTYNVRGLSWPLANGRAKALKAIGAELATMRRYGQQPDVVLIQEGFRGEVGDLVT
                     ASGYRYWARGPGRSQKASGARPESGKRYATVRYPSFGEGWGKFTGAGLHALSDAPITD
                     VEDTPYRYCAGLDCLANKGVMLVRLALPGAPSQIDIVNTHLNSRRAAKVPVARSLQAH
                     NLQTEELLTFINAHRDVDRPLLVGGDFNVRNAPDRYDYRSAERPFQVVSEFCSRAVNR
                     CEGQAVDSPAARPWLRSQDLQAFASAGPVTVRPIRIETLFGASKSGPKLSDHDGYLVR
                     YRLSWDPAIVAAAPPRPPVELRPQFRSGLGLKVTWRR"
     gene            complement(1958..>2689)
                     /locus_tag="PLH31_08985"
     CDS             complement(1958..>2689)
                     /locus_tag="PLH31_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HQR89380.1"
                     /translation="TDALDASSFPLVPFCNRIPNGRFTFHGRAVVLRPNLGDHPHPLH
                     GQGWRGPWTVESASGAQARLAYDHAPSDWPWAYRAEQRFILGERGLRLELSVTNTGDA
                     PMPAGLGFHPYFPRRSGEVLEASVSGVWMIDQDVLPTVHHEGVWGPDWTRGAGVEGHD
                     DLIDHCYTGWNQRAALSAPGQPTTTITASADCRWLHLYVPPGQDFYCVEPCANRPNPF
                     GSPGSCMVILAPGQARAIWMEIATA"
BASE COUNT          426 a          902 c          940 g          411 t
ORIGIN      
        1 gcggctggaa gcccggcgtc ggctgggccg aggaagtgat cctgcccccg ggcttcgact
       61 atggcctgtc ggaaggtccg cgcgagctgc cctcgtggtg ggaagccagg ggggtccgac
      121 gcgccgacgg cctgccctgg agcgccgccg acgcggcctc tcccgccggc ctgatcctgc
      181 cggccggcgc ctcggggccc gccttcctgg ccctgccgaa ccattttgcg atccgnnnnn
      241 nnnnncctcc tacgccctgg gcatcggcct gctggctgac cgcttcggcg gcggcgaccc
      301 gatcatcggc gcctggccgg tcgatgcgcc gctgtccctg tcagaccgca tggccgccca
      361 gatcgccctg gcgcgtctgg gctttgatcc cggcgctccg gacggcgtgg tcggggccgg
      421 cacccgcaag gccctgcgcg cctggcagca gagccgaggc ctgccggccg acggctatat
      481 gtcgatggac atggtccagc gcctgaaagc ccaggcagga ctgagctagg tccacgctcc
      541 ggcctttttc cggcaaggcc aaagcgcgat cggggcctag cgccgccaag tcaccttcag
      601 gcccaggccg ctgcggaact ggggccgcag ctccacgggc ggccgtgggg gcgcggccgc
      661 cacaatggcg gggtcccagc tgagccggta gcgcaccaga tagccgtcgt gatccgatag
      721 ctttggcccc gacttggaag caccgaacag cgtctcgatc cggatcggcc gcacggtcac
      781 cggcccggcg ctggcaaagg cctgcaggtc ctgagagcgc agccagggtc gggcggccgg
      841 gctatccacg gcctggcctt cacagcgatt gacggcgcgg ctgcagaatt cgctgaccac
      901 ctgaaacggc cgctcggcgg atcgatagtc gtagcggtct ggcgcgttgc gaacattgaa
      961 gtcgccgccg accagcagcg gccgatcgac atcgcgatgg gcgttgatga aggtcaacag
     1021 ctcttcggtc tgcaggttat gagcctgcag cgaccgggcg accggcacct tggcggcgcg
     1081 acgcgaattg agatgggtat tgacgatgtc aatctggctg ggcgcaccgg gaagggccag
     1141 gcgaaccagc atcaccccct tgttggccag acagtcgagg cctgcgcagt agcggtaggg
     1201 cgtatcctcg acgtccgtga tcggcgcgtc gctcagcgcg tgcagaccgg cgccggtaaa
     1261 cttgccccag ccctctccga atgacggata gcgcaccgtg gcgtaacgct tcccgctctc
     1321 cggccttgcc cccgaggcct tctgggaccg gccggggccg cgggcccagt agcggtagcc
     1381 gctggcggtg accaggtcgc cgacctcccc gcgaaagcct tcctgaatca gcaccacgtc
     1441 gggctgctgg ccgtagcgcc tcatcgtcgc cagttcagcc ccaatcgcct tcagagcctt
     1501 ggcgcgaccg ttggccagcg gccaggacag accccggaca ttgtaggtga gcacgctgat
     1561 ctctgcggtc ggcgcgcaac cgggcggggc cacactgccg ctcggtgtgg cgccgggcat
     1621 ggcgccaaga tcggttacgg gggccagggc cgccagggcg gtaaccgggg gcaaagtcgc
     1681 caaagtcatc atttcgccgc tcccagacat gcgggccgcg ttgaaagaca atccagaccg
     1741 tgctcgtccc caaggcggcg cgtagttcag acgcagccgg atgtgtactg agttcttgcc
     1801 ggcgagatgg tctctctgct gattcggaga cggcgcagtg aatctgacgc gacggtttta
     1861 ttgtgatggc tctggcaagc ccgcaggcct tgtctggcaa gctctggggc gcacgcggac
     1921 aaatcgtcgc ggcgccgcaa atcagacttt gggagtctta ggcggtggcg atctccatcc
     1981 agatcgcgcg agcctggccc ggcgccagga tgaccatgca ggagcccggt gacccgaagg
     2041 gattgggccg gttggcgcag ggttcgacgc aatagaagtc ctggccgggc ggcacataca
     2101 ggtgcagcca gcggcaatcg gccgaggcgg tgatcgtggt ggtgggctga cccggcgcgc
     2161 tcagggcggc gcgctggttc cagccggtgt aacagtgatc gatcagatca tcatggcctt
     2221 cgaccccggc gccgcgggtc cagtccggtc cccagacgcc ctcgtgatgg acggtgggca
     2281 gcacgtcctg atcgatcatc cacacgccgc tgaccgaggc ctcgagcacc tcgcccgagc
     2341 ggcgcgggaa atagggatgg aagcccaggc cggccggcat gggcgcatcg ccggtattgg
     2401 tcactgaaag ctccaggcgc aggccgcgct cgcccaggat gaaccgctgc tcggcgcgat
     2461 aggcccacgg ccagtccgac ggcgcgtggt cataggcgag ccgcgcctgg gcgcctgagg
     2521 cgctctcgac ggtccagggc ccacgccagc cctgaccatg cagcggatgc ggatgatcgc
     2581 ccagattggg cctcaggacc acggcgcgcc cgtggaacgt gaaccggccg ttcggaatgc
     2641 ggttgcagaa cgggaccagc ggaaagctgg acgcatccag cgcatcggt
//
LOCUS       DAOMEO010000362         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4087_length_2683_cov_2.038052, whole genome shotgun sequence.
ACCESSION   DAOMEO010000362 DAOMEO010000000
VERSION     DAOMEO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4087_length_2683_cov_2.038052"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLH31_08990"
     CDS             <1..908
                     /locus_tag="PLH31_08990"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HQR89381.1"
                     /translation="TTRSLRRLFPDEARFAVHDPLPAQPHFADEGAANHVRLCAEHGA
                     PGVNLFVWGREAWEHWDGRYPARQTREAFEAVARRHGAARAIFPRQGKAAINGGAFHN
                     DVVCVGTRQCLFFHERAFEDRIGMEAAVRAAAEGLFEPAFVEISEADLPMADLVASYL
                     FNSHLLVILGEDRLVLLAPAETRDNPRAHAVAQSLATSNGPIGRVDYVDVRQSMRNGG
                     GPACLRLRVVLTEDELAATNPAQRFDAALHARLTDWVERCYRDRLAPADLADPALLTE
                     VREALDELTGILDLGGDFYPFQRTA"
     gene            905..1396
                     /locus_tag="PLH31_08995"
     CDS             905..1396
                     /locus_tag="PLH31_08995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR89382.1"
                     /translation="MSYQIRAATPGDVTALVDLHRAAAAVPDGLARRPDEITRDYVER
                     FIAGISFVAVDPDGAIWGEIHGARETVALFAHVLSNLTVAVHPDRQGLGIGSKLFEAL
                     IAHARTMDPPILRIELAAGAGNLGAIRLYERLGFRHEGRQVARGRYPDGRLEDDILMG
                     MLI"
     gene            complement(1393..>2214)
                     /locus_tag="PLH31_09000"
     CDS             complement(1393..>2214)
                     /locus_tag="PLH31_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQR89383.1"
                     /translation="GTSGALFGMSGDERRAILRAWLLGIGLIGTICFVNILTIQHDDP
                     DLGALPPVIWEGSSALVTLAIFAIPAAVALWTHRHRPRWWQAAPVHLIAVLTYSALHV
                     AGFVALRKLAYLVLLQQTYDFGDLYREVPYEFRKDIVAYGIAWVLFFLSLRQGRQQAA
                     ARPAPLPATFDIQDGARLLRVPIDDILAVRSAGNYVEFVLADSRRPLMRTPLAAIQTD
                     LTEHGFIRSHRSWLVNKARVTGLRPEGSGDYAVELGELEAPLSRRFPAALAALRG"
     assembly_gap    2215..2224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2345..>2683
                     /locus_tag="PLH31_09005"
     CDS             2345..>2683
                     /locus_tag="PLH31_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQR89384.1"
                     /translation="MTTTTSSLDRRYDLDWIRVGAFFLLILYHVGMFYVPWDWHVKSP
                     QPVEALEPLMQLTNPWRLTLLFLVSGAATRFMADKVSVGKLTGARIARLLPPLVFAMF
                     VIVPPQSYYQV"
BASE COUNT          414 a          893 c          917 g          449 t
ORIGIN      
        1 agaccacccg cagcctgcgt cggctgtttc cggacgaagc ccggttcgcc gtgcacgatc
       61 ctctgcccgc ccagccgcac ttcgccgacg aaggcgcggc caaccatgtc cgcctctgcg
      121 ccgagcatgg cgcgcccggc gtcaacctgt tcgtctgggg gcgcgaggcc tgggagcact
      181 gggacggtcg ctatccggcc cgccagaccc gcgaggcctt cgaagccgtg gcgcgtcgcc
      241 acggggcggc gagggccatc ttcccgcgcc agggcaaggc ggcgatcaat ggcggggcgt
      301 tccacaatga cgtcgtctgc gtcggcacgc gccaatgcct gttcttccac gaacgcgcct
      361 ttgaggatcg gatcggcatg gaagccgcgg ttcgcgcagc ggccgagggt ctgttcgaac
      421 cggcctttgt cgagatctcc gaggccgatc tgccgatggc cgatctggtg gccagctacc
      481 tgttcaattc gcatctgttg gtcatcctcg gcgaggaccg tctggtgctg ctggcccctg
      541 cggagacccg tgacaacccc cgcgcccacg cggtggcgca gagcctggcg acgtccaacg
      601 gtcccatcgg ccgggtcgac tatgtcgacg tgcgccagag catgcgtaat ggcggcgggc
      661 ctgcctgcct gcgtctgcgc gtggtgctga ccgaagacga actggccgcg accaatccgg
      721 cccagcggtt cgatgcggct ctgcatgcgc gcctgaccga ctgggtcgag cgctgctatc
      781 gcgaccgcct ggccccggcc gacctggctg atcccgcctt gctgactgag gtgcgcgagg
      841 ccctggacga gctgaccggc atcctcgacc ttggcggcga cttctatccg ttccagagga
      901 ccgcatgagc tatcagatcc gggcggcgac gcccggcgac gtcaccgcgc tggtcgacct
      961 gcaccgcgcc gccgccgctg tgcccgacgg gttggcgcgt cgtcccgacg agatcacgcg
     1021 ggactatgtg gagcgtttca tcgccgggat cagctttgtg gccgtcgacc ccgatggcgc
     1081 gatctggggc gagatccacg gtgcgcgcga gacggtggcg ctgtttgctc atgtcctgtc
     1141 aaacctgacc gtcgccgtgc atcctgatcg gcagggtctg ggcatcggct ccaagctgtt
     1201 cgaggccctg atcgctcacg cccggacgat ggacccgccg atcctgcgga tcgaactggc
     1261 ggcgggggcg gggaatctcg gggcgatccg gctgtatgag cggctgggct tcaggcacga
     1321 ggggcggcag gtcgcgcgcg ggcgctatcc tgacggccgt ctcgaagacg atatcctcat
     1381 ggggatgctg atctagccgc gcagggcggc cagggccgca gggaagcggc gcgagagcgg
     1441 ggcctccagc tcacccaact cgacggcgta gtcgcccgag ccctccggac gcaggccggt
     1501 gaccctggcc ttgttgacca gccacgagcg gtggctgcgg ataaaaccgt gctcggtcaa
     1561 atctgtctgg atcgcggcca gcggcgtgcg catcagcggc cggcggctgt cggctagcac
     1621 gaactcgaca tagtttccag ccgagcgcac ggccaggatg tcgtcgatgg gcaccctgag
     1681 caaccgcgcg ccatcctgga tgtcgaaggt ggccggcaac ggggctgggc gagcagcggc
     1741 ctgctggcgt ccctgccgga gggagaggaa aaacaagacc caggcgatgc cgtaggccac
     1801 gatatccttg cgaaattcgt aggggacttc tctgtagagg tcgccgaagt cgtaggtctg
     1861 ttgcagcagg accagatagg ccagcttccg cagcgccacg aagccggcca catgcagggc
     1921 cgaataggtc aagaccgcga tcagatgcac gggcgcggcc tgccaccaac gcggccggtg
     1981 gcgatgggtc cagagcgcga ccgcggcggg gatggcgaag atcgccagcg tgaccagcgc
     2041 gctgctgccc tcccagatga ccggaggaag agcgccaagg tcgggatcgt cgtgctggat
     2101 cgtcaggatg ttgacgaagc agatcgtccc gatcaggccg atgcccagaa gccaggcccg
     2161 caggatcgcc cggcgctcgt cgccgctcat cccgaaaagc gcgccgctcg tcccnnnnnn
     2221 nnnngctcgt cccggctggt tttccgttcg tccccggcaa ggtccgccga tcccgaggtg
     2281 cttggcgggg ctgggcgtca cgaggcacga ggttcgcatc ccttttcaag ctgggcgcct
     2341 cttcatgacc acaacgactt catccctgga ccgccgctat gacctggact ggatccgggt
     2401 tggcgcgttc ttcctgctga ttctgtacca cgtcggcatg ttctacgtac cgtgggactg
     2461 gcacgtgaag agcccgcagc cggtcgaggc gcttgagccg ctgatgcaac tgaccaatcc
     2521 ctggcgactg accctgctgt tcctggtttc aggcgccgca acccggttca tggccgacaa
     2581 ggtcagcgtc gggaagctga ccggcgcccg gatcgcgcgg ctgctgccgc cgctggtctt
     2641 cgcgatgttc gtgatcgtgc cgccgcagtc ctattatcag gtc
//
LOCUS       DAOMEO010000363         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4090_length_2681_cov_2.458492, whole genome shotgun sequence.
ACCESSION   DAOMEO010000363 DAOMEO010000000
VERSION     DAOMEO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4090_length_2681_cov_2.458492"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLH31_09010"
     CDS             <1..234
                     /locus_tag="PLH31_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HQR89385.1"
                     /translation="GEQQRVALARALAARPILLFADEPTGNLDSRNAGSVVDLMFDLL
                     AECGAALVMVTHDPVLAERADRIVRMADGKVVA"
     gene            231..>2681
                     /locus_tag="PLH31_09015"
     CDS             231..>2681
                     /locus_tag="PLH31_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQR89386.1"
                     /translation="MSALSFRLAARELRSGVRGFGIFLACLALGVAAIAAASSTAEAF
                     RRGLASQAREILGGDISTSVENRDFTPQERATFARLGTVSYTARVRVMAQAPSGERRL
                     VDLRGADSAYPLAGKVELEGAASLAEALAVRDGLPGAAVEPALLDRLGLKIGDRFEAG
                     PLTLVVRAVMIRQPDSVARGFVLGPRVVVRRDLLDPTGLLAPGLPSDQAARIAVRDGV
                     DPVAAGKQLEKSFGEAGLGVRDRSNAAPGAKRLIDQLEYFLGFIGLASLVAGGLGVAG
                     AVSAYLATREPSFAVLKALGADGALIRNTYLIQIGLLASLGVTLGLVIGAIVPLILGQ
                     IAGSELPIPALFAVYPLPLVKAGAFGLLAAAAFSLIPLARARATPPSALFRRALGVRP
                     PFGLEMIGATLAGLGLAALAVATAPTPIAAAIMITGVAVAFVALWALGLLAAWLAGKL
                     RGLARGPMRIGLANLAGPRSAARTATPAIGLGVALLACVVLIQSALLAQVRDVAPKSA
                     PAVVFTEIPGDQAAAFDAEVFRAAGRLDADSYLRLPFATGRIIALNGQPVDINAIKPS
                     ERWAFDNDVNLSVLDAEPKDSGIVAGRWWPAGYTGPPQVALEVDLARGAGLKAGDTIT
                     LSVLGRDLQVTVAVLREVEFGGFGPNFSVVLDPKSLEGAVLRNIAIARMDRPAEARLT
                     RALGQSFPAINVISVREQLEAAAGLFDRLALAIRGAAAVAGLAGLLVLAGAIAAGARA
                     RAREAATLKVLGGTRTQILSAYVVEYGAVGLIAGAAGVLLGLAAAWPVVVLVFKSEWS
                     IDWAGVMALLAGAAGLSAI"
BASE COUNT          287 a          966 c          987 g          441 t
ORIGIN      
        1 ggcgagcagc agcgcgtcgc cctggcccgc gccctggcgg ctcgcccgat cctgctgttc
       61 gccgacgagc cgaccggcaa tctggacagc cgcaatgccg gctccgtcgt ggatctgatg
      121 ttcgacctgc tggccgagtg cggcgcggcc ctggtgatgg tcacccatga ccccgtgctg
      181 gccgagcgcg ccgaccgcat cgtgcgcatg gccgacggca aggtggtcgc gtgagcgcct
      241 tgagcttccg cctcgccgcc cgtgaactgc gttcgggcgt tcggggcttt ggcatcttcc
      301 tggcctgcct ggcgctgggt gtcgccgcca tcgcggcggc cagctccacc gccgaggcct
      361 tccgccgggg cctggcgtcg caggcgcgcg agatcctggg cggcgatatc tcgacctcgg
      421 tcgagaaccg cgatttcacg ccgcaggaac gcgccacctt cgcccggctg ggcacggtgt
      481 cctacacagc ccgcgtccgg gtgatggccc aggctccatc cggggaacgc cggctggtcg
      541 acctgcgcgg cgccgacagc gcctatcccc tggccggcaa ggtcgagctg gagggcgcgg
      601 cgagcctggc cgaggctctt gctgtccgcg atggcctgcc cggcgcggcg gtcgagcccg
      661 ccctgctgga tcgcctgggc ctgaagatcg gcgacaggtt cgaggcaggt cccctgaccc
      721 tggtggttcg cgccgtgatg atccgtcagc ccgacagcgt ggcgcgcggc tttgtgctgg
      781 gcccccgcgt ggtggtgcgt cgcgacctgc tggacccgac gggcctgctg gcccccggcc
      841 tgccgtccga tcaggccgct cgtatcgccg tgcgcgacgg cgtcgatccg gtcgccgccg
      901 gcaagcaact ggaaaagtcc tttggcgagg ccggtctggg cgtgcgcgat cgctcgaacg
      961 ccgcgccggg cgccaagcgc ctgatcgatc agctggaata cttcctgggc tttatcggcc
     1021 tggcctcgct ggtggcgggc ggtctgggcg tggccggcgc ggtctcggcc tatctggcga
     1081 cccgcgagcc gtcgttcgcg gtgctaaagg cccttggcgc ggacggggcg ctgatccgca
     1141 acacctatct gatccagatc ggcctgctgg ccagtctggg cgtcaccctg ggtctggtga
     1201 tcggcgcgat cgtgccgctg atcctgggtc agatcgccgg cagcgaattg ccgatcccgg
     1261 ccctgttcgc ggtctatccc ctgccgctgg tcaaggccgg cgcctttggc ctgctggcgg
     1321 cggcggcctt ctcgctgatc cccctggccc gcgcccgcgc caccccgccc tcggccctgt
     1381 ttcgccgggc gctgggcgtg cggccgccgt ttggcctgga gatgatcggc gccaccctgg
     1441 ctggtctggg cctggccgcc ctggcggtcg ccaccgcccc caccccgatc gcggccgcca
     1501 tcatgatcac cggcgtcgcc gtggcctttg tcgccctttg ggcgctgggc ctgctggctg
     1561 cctggctggc gggcaagctg cgcggcctgg cgcgcggacc gatgcggatt ggtctcgcca
     1621 acctggccgg accgcgctcg gcggcccgca ccgccacccc ggccatcggc ctgggcgtgg
     1681 ccctgctggc ctgcgtggtg ctgatccagt cggccctgct ggcccaggtg cgcgatgtgg
     1741 cccccaagag cgccccggcc gtggtgttta cggagatccc cggcgatcag gcggccgcct
     1801 ttgacgccga ggtcttccgc gccgccggac gcctggacgc cgacagctat ctgcgcctgc
     1861 ccttcgccac cggccggatc atcgccctga acggccagcc cgtggatatc aacgccatca
     1921 agccgtccga gcgctgggcg ttcgacaacg acgtcaacct gtcggtgctg gacgccgagc
     1981 ccaaggattc agggatcgtc gcgggccgct ggtggcctgc gggctacacg ggtccgcccc
     2041 aggtggcgct ggaggtcgat ctggcgcgcg gcgcgggcct gaaggccggc gacaccatca
     2101 ccctgtcggt gctgggccgc gatctgcaag tcacggtcgc cgtgctgcgc gaagtggaat
     2161 tcggcggctt tggccccaac ttctcggtgg tgctggaccc caagagcctg gaaggcgcgg
     2221 tgttgcgcaa tatcgccatc gccaggatgg accgcccggc cgaggcccgc ctgacccgcg
     2281 cgctgggcca gagcttcccg gccatcaatg tgatcagcgt gcgcgagcag ctggaggccg
     2341 ccgccggcct gttcgaccgc ctggccctgg ccatccgggg cgcggcggcg gtggcgggcc
     2401 tggccggcct gctggtgctg gccggggcca tcgcggccgg ggccagggcc cgggcgcgcg
     2461 aggcggcgac gctgaaggtg ctgggcggca cccgcaccca gatcctgtcg gcctatgtgg
     2521 tggaatatgg cgcggtgggc ctgatcgccg gcgcggccgg cgttctgctg ggcctggccg
     2581 ccgcctggcc ggtggtggtg ctggtgttca agtcagagtg gagcatcgac tgggccgggg
     2641 tgatggccct gctggccggc gcggcgggcc tgtcggcgat c
//
LOCUS       DAOMEO010000364         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4091_length_2681_cov_2.181645, whole genome shotgun sequence.
ACCESSION   DAOMEO010000364 DAOMEO010000000
VERSION     DAOMEO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_2681_cov_2.181645"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PLH31_09020"
     CDS             complement(<1..1888)
                     /locus_tag="PLH31_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQR89387.1"
                     /translation="MRHSEVTLDDKYLLEDGRAFITGVQALIRVLLDRKRLDRKAGLN
                     TAGFLSGYRGSPLGGLDQQAARLNKLLTAHDVVFKEGLNEDLAATAVWGSQQANLFPG
                     ATHDGVFGMWYGKAPGVDRTGDVFKHANFAGSFPTGGVLAVAGDDHACKSSTLPSQSE
                     FAFQDFEMPVLSPADVQEVLDYGLMGIAMSRFTGLWTGMIALADTMDSGVTIDVSLDR
                     HRIITPENFPLPPGGLGIRLKDQPMEKERRLRLHKIPAALAFARANHIDRVVLGSSHV
                     RVGKARLGIVCQGQAYKDVLEAFTAMGMTLQEAADLGVSIYKVGMPWPLEPLGIRAFA
                     AGLETLMVIEHKRGLIEPQARAALYDLPAQARPRIIGKTDEHHHPLLSELGSLSVAEI
                     ALAIYDRLPDGPHMERARAYLDRVSAAGVAAVSLAADQARKPFFCSGCPHNTSTKLPE
                     GSRALAGIGCHYMAGFNDPSTDLNTHMGGEGLTWVGAAPFTTEKHVFQNLGDGTYNHS
                     GSLAIRAAIAAKANITYKLLFNDAVAMTGGQKAESGFTPAQITRQLASEGVTKTVIVV
                     DELERYAEVKDLAPGVEVFPRSELMTVQKMLRDTSGTTVLLYDQTCATEKRRRRKRGT
                     MAKAT"
     gene            2030..2527
                     /locus_tag="PLH31_09025"
     CDS             2030..2527
                     /locus_tag="PLH31_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR89388.1"
                     /translation="MSEQLDAVDAKILDLIQHDAGLSVAEIAERVGLSSSPCWRRIKR
                     LEDAGVIQRRVTILDREKLGLGFEVYCTVKLSLPTKENLDTFEQAVGKWAEVVQCATV
                     TGAADYEMRIVTRDMRAFDEFLRDKLLSLGLVSNIESRIVIRGVKNSTAVPLGLVSPY
                     VSPLG"
BASE COUNT          433 a          869 c          920 g          459 t
ORIGIN      
        1 gggtcgcctt ggccatggtg ccgcgcttgc ggcggcggcg cttttcggtg gcgcaggtct
       61 ggtcgtagag cagcaccgtg gtgccggacg tgtcgcgcag catcttctgc acggtcatca
      121 gctcggatcg tgggaacacc tcgacgccag gcgccagatc cttgacctcg gcatagcgct
      181 ccagctcgtc gacgacgatg accgtcttcg tcacgccttc agacgccagc tggcgggtga
      241 tctgggcggg ggtgaagccg ctctcggcct tctggccacc ggtcatggcg acggcgtcgt
      301 tgaacagcag cttgtaggtg atgttggcct tggccgcgat cgccgcccga atcgccaggg
      361 agccagagtg gttgtaggtg ccgtcgccca ggttctggaa gacgtgcttc tcggtcgtga
      421 acggcgccgc gcccacccag gtcaggccct cgccgcccat gtgggtgttg aggtcggtcg
      481 agggatcgtt gaagccggcc atgtagtggc agccgatgcc ggccagggcc cgcgagcctt
      541 ccggcagctt ggtcgaggtg ttgtgcgggc agcccgagca gaagaacggc ttgcgggcct
      601 ggtcggcggc caggctgacg gcggcgacgc cggcggccga gacgcggtcc agataggcgc
      661 gggcccgctc catgtgcggc ccgtcgggca gacgatcata gatggccagg gcgatttcgg
      721 cgaccgacag cgagcccagc tccgacagca gcgggtgatg gtgctcatcg gtcttgccga
      781 tgatgcgggg gcgagcctgg gcgggcaggt catagagggc ggcgcgcgcc tggggctcga
      841 tcaggccacg cttgtgttcg atcaccatca gggtctcaag accggcggcg aaggcgcgga
      901 tgcccagggg ctccagcggc cacggcatgc cgaccttgta gatcgacacg cccagatcgg
      961 cggcttcctg cagggtcatg cccatggccg tgaaggcctc cagcacatcc ttgtaggcct
     1021 ggccctggca gacgatgcca aggcgggcct tgccgacgcg gacgtgggat gagcccagca
     1081 ccacccggtc gatgtggtta gcgcgggcga aggccagggc ggcggggatc ttgtgcagac
     1141 ggagccggcg ctccttctcc atcggctggt ccttcagccg gatgcccaga ccgccgggcg
     1201 gcagggggaa gttttcgggc gtgatgatcc ggtggcggtc cagcgagacg tcgatggtca
     1261 cgccgctgtc catggtgtcg gccagggcga tcatgccggt ccacaggccg gtgaaccgcg
     1321 acatcgcgat ccccatcagg ccgtagtcca gcacttcctg cacgtcggcc ggcgacagca
     1381 ccggcatctc gaagtcctgg aaggcaaatt ccgactgcga cggcagggtc gagctcttgc
     1441 aggcatggtc gtcgccggcc acggccagca cgccgccggt cgggaagctg ccggcgaaat
     1501 tggcgtgctt gaagacgtcg ccggtgcggt cgacgccggg cgccttgccg taccacatgc
     1561 cgaacacgcc atcatgggtc gcgcccggga acaggttggc ttgctggctg ccccacacgg
     1621 cggtggcggc caggtcctcg ttgaggcctt ccttgaagac cacatcatgc gcggtcagca
     1681 gtttgttcag gcgcgcggcc tgttgatcga gaccgcccag gggcgagccg cgatagcccg
     1741 acaggaagcc tgccgtattc aaccccgcct tgcggtcgag gcgcttgcga tccagcaaaa
     1801 cccggatcag cgcctgcacg ccggtgatga aggcgcgacc atcttcgaga agatatttgt
     1861 cgtctagagt gacttccgag tgcctcatgg caaaaaaatt cctcccgggt cttctcgccc
     1921 gcgtagcaat atcttataga aacagggaaa ttgtttgccc aaggcgtgcc ctacccatgg
     1981 ccgctttcgg cacgatcgcc gcgccaaatg acctctgggg gaggaattct tgtccgaaca
     2041 actcgacgcc gtggatgcca agatcctcga tctcatccaa cacgacgccg gactgtccgt
     2101 cgccgagatc gctgagcgag tcggattgtc gtccagcccg tgctggcgcc ggatcaaacg
     2161 gctggaagac gccggcgtca tccagcgtcg cgtgaccatt cttgaccgtg aaaagcttgg
     2221 cctgggcttt gaggtctact gcaccgtcaa gctctcgctc ccgacaaagg aaaacctcga
     2281 caccttcgag caggccgtcg gcaagtgggc tgaagtggtg cagtgcgcca ccgtgaccgg
     2341 cgccgccgac tacgagatgc gcatcgtcac ccgcgacatg cgcgcgttcg acgagttcct
     2401 gcgcgacaag ctgctgtcgc tgggtctggt gagcaacatc gagagccgca tcgtcatccg
     2461 gggggtcaag aactccaccg ccgtgccgct gggtctcgtc agcccctatg tcagcccgct
     2521 gggctaaggc gctcgcgagc gtcgccctcg gcgcgctcgc cttttgcctg accacgccgt
     2581 ccctggccga gggacggctg gacgctttcc gccgcctgcg cgccgagggc ctggccgccg
     2641 ccaaggccgg cgacatggcc gccgctcagg cccgggtcag c
//
LOCUS       DAOMEO010000365         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4100_length_2676_cov_2.657764, whole genome shotgun sequence.
ACCESSION   DAOMEO010000365 DAOMEO010000000
VERSION     DAOMEO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4100_length_2676_cov_2.657764"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLH31_09030"
     CDS             complement(<1..1228)
                     /locus_tag="PLH31_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR89389.1"
                     /translation="MAWDFETDPDFAEQLAWIDTFVREEVEPLDLILQSPFDVDNPLN
                     RQFVRPLQQQVKDRGLWACHLGPQLGGPGYGQVKLALMNEILGRSIFAPTVFGCQAPD
                     SGNAEILAVYGTAEQKARYLQPLLANDIVSCFSMTEPQGGADPTTFSARAVMKGEQWV
                     LNGEKWFATNARYASFFLVMAVTDPDAPPHRRLSVFIIPKGAPGLEIVRNVGYGDRDP
                     AHAYLRFTDVGVPLDHMLGGQGDGFVVAQVRLGGGRIHHAMRTIGKARQAFDMLCDRA
                     LSRITRSEPLADKQLVQEKIADSWIELEQFRLLVLRTAWKIDREQDYNKVRGDISAIK
                     AAMPKVLHDIAARAVYVHGCLGLSNETPLAAMVLDSFQVGLADGPTDIHKVTLAKQLL
                     RGRKHSQGLFPDYHIPA"
     gene            complement(1253..1984)
                     /locus_tag="PLH31_09035"
     CDS             complement(1253..1984)
                     /locus_tag="PLH31_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89390.1"
                     /translation="MDLDFTGKRVLIIGGSSGIGLACAQGFAARGAEVAVTGTRANKT
                     AYGDAASALEPFAFSTLNVSTPGAVERWIPPFETLDVVILSQGAVEYRRKEFEADAFR
                     HVVEVNLTSLMACAQKLAPALEARSGSLIVISSVGGLRATRGNPAYSASKAGAIHLTR
                     TLGDAWAGRGIRVNGIAPGLIATKMTAVTTADPARLQERLKGIPLNRLGHPEDVAGVA
                     LFLASPLAAYIVGETIVVDGGRTLS"
     gene            1910..>2676
                     /locus_tag="PLH31_09040"
     CDS             1910..>2676
                     /locus_tag="PLH31_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR89391.1"
                     /translation="MGAGQPDPARSADDQDALSGEVEVHDGLKRLNETVALFSMPRLN
                     RQSMKADIARDAIKRAAQSLFAARGVDAVSVRDILTATGQRNGASLHYYFGSKDDLVA
                     QLVIDGARLIDDRRNARLDALEADGGPHSLREVMEVLILPSTDLGRDGGEGDYLRFIS
                     TFSLQNRATFDAIVGEGWNRGYQRCLDHIRRLSPAPPEVLERRLVFLSLGLRAIMTAR
                     EAALAVHPDHAFWGPAATLDDLVETLVGMVTPDETHLR"
BASE COUNT          445 a          846 c          922 g          463 t
ORIGIN      
        1 gcgccgggat gtgatagtcg gggaacaggc cctgcgagtg tttgcggccc cgcaggagct
       61 gcttggccaa ggtcaccttg tggatgtcgg ttggcccgtc ggctaggccg acctggaagg
      121 agtccagcac catcgcggcc aatggggtct cgttcgatag ccccaggcat ccatggacgt
      181 agacggcccg cgcggcgatg tcgtgcagca ccttgggcat ggccgccttg atggcggaaa
      241 tgtcgccgcg caccttgttg tagtcctgct cgcggtcgat cttccaggcg gtgcgcagga
      301 ccagcaagcg gaactgctcg agctcgatcc agctgtcggc gatcttctcc tggaccaact
      361 gcttgtcggc cagcggctcg ctgcgcgtga tgcgcgacag ggcgcggtcg cacagcatgt
      421 cgaaggcctg gcgcgccttg ccgatggtgc gcatggcgtg gtgaatgcgg ccgccgccca
      481 gccggacctg ggccacgacg aagccatccc cctgaccgcc cagcatatga tccagcggca
      541 ccccgacatc ggtgaagcgc agataggcgt gggccggatc gcggtcgcca tagccgacgt
      601 tgcgaacgat ctcgaggcct ggcgcgccct tgggaatgat gaagaccgac agtcggcgat
      661 gcggcggcgc gtcgggatcg gtcacggcca tgaccaggaa gaagctggcg tagcgggcat
      721 tggtggcgaa ccatttctcg ccgtttagca cccattgttc gcccttcatc acggcgcggg
      781 ccgagaaggt cgtcgggtcg gcgcctcctt gcggctccgt catcgagaag caggagacga
      841 tgtcattggc gagaaggggc tgaagatagc gcgccttctg ctcggcggtg ccatagacgg
      901 cgaggatctc cgcgttgccg gaatcgggcg cctggcagcc gaagacggtg ggggcgaaga
      961 tcgagcggcc gaggatttca ttcatcaggg ccagcttgac ctggccatag ccagggccgc
     1021 ccagttgggg cccaagatgg caggcccaga ggccgcgatc cttgacctgc tgctgcagcg
     1081 gccggacgaa ctgtcggttc aagggattgt cgacgtcgaa cgggctctgc aggatcaggt
     1141 cgagcggctc gacctcttcg cggacgaagg tgtcgatcca ggccaactgc tcggcgaaat
     1201 cggggtcggt ctcgaagtcc caggccatcg gccgtctccg ttcgttgtga tctcaggata
     1261 gggttcggcc gccatcgacg acgatggtct cgccgacgat gtaggccgcg agcggagagg
     1321 ccaggaacaa cgccacaccg gcgacgtcct cggggtgacc caaccgattg agaggaatgc
     1381 ccttgaggcg ttcctgaagc cgcgcgggat cggcggtcgt gaccgcggtc atcttggtgg
     1441 cgatcaggcc gggcgcgatg ccgttgacgc gaatgcctcg tccggcccag gcgtcgccca
     1501 gggttcgggt cagatggatg gcgcccgcct tcgacgccga ataggccggg ttgccgcgcg
     1561 tcgcgcgcag gccgccgacc gagctgatca cgatcaggct tccgcttcga gcctcgagcg
     1621 cgggcgccag tttctgggcg caggccatca ggctggtcag attgacttcc accacatgac
     1681 gaaaggcgtc cgcctcgaac tccttgcggc ggtactccac cgcgccctgg gacaggatca
     1741 cgacatcaag ggtctcgaag ggcgggatcc aacgctcgac cgcgccgggt gtggagacgt
     1801 tcagggtgga gaaggcgaac ggctccagcg ccgaggcggc gtcgccatag gcggtcttgt
     1861 tcgcgcgggt tcccgtcacg gcgacttcgg cgccccgggc ggcgaagcct tgggcgcagg
     1921 ccaacccgat cccgctcgat ccgccgatga tcaggacgcg ctttccggtg aagtcgaggt
     1981 ccatgacggc ctaaaacgat tgaatgaaac agttgcttta ttttccatgc ctaggttaaa
     2041 ccgtcaatcc atgaaagccg atatcgcccg ggacgcgatc aagcgggccg ctcagagcct
     2101 gttcgccgcg cgcggcgtgg acgccgtctc ggtgcgcgac atcctgaccg cgaccggtca
     2161 gcgcaacggc gcctcgctgc actattattt cggatcgaaa gacgacctgg tcgcccagct
     2221 cgtcatcgac ggcgctcgcc tgatcgacga tcgccgcaac gcccggctcg atgcgctcga
     2281 ggccgatggc ggcccccatt cgctgcggga ggtaatggag gtgctgatcc ttccgtcgac
     2341 cgatcttggc cgcgacggag gcgaggggga ctatctgcgt ttcatcagca ccttcagcct
     2401 gcagaaccgc gcgaccttcg acgccatcgt cggcgaaggc tggaaccggg gctatcagcg
     2461 gtgcttggac cacatccgtc ggctctcccc ggcgccgcct gaggtgctgg agcgtcgact
     2521 ggtgttcctc agcctggggc tgcgcgcgat catgacggcg cgagaggccg cgcttgccgt
     2581 tcatccggac cacgcgttct ggggtccggc cgcgacgctg gacgatctgg tcgagactct
     2641 cgtgggcatg gtcacgcccg atgagactca tcttcg
//
LOCUS       DAOMEO010000366         2672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4106_length_2672_cov_2.426060, whole genome shotgun sequence.
ACCESSION   DAOMEO010000366 DAOMEO010000000
VERSION     DAOMEO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4106_length_2672_cov_2.426060"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="hutU"
                     /locus_tag="PLH31_09045"
     CDS             complement(<1..811)
                     /gene="hutU"
                     /locus_tag="PLH31_09045"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQR89392.1"
                     /translation="MTRIDNSRVIRAATGTQLTAKSWLTEAPLRMLMNNLHPDVAERP
                     EELVVYGGIGRAARDWESFDKIVETLRRLEDDETLLVQSGKPVGVFRTHTDAPRVLIA
                     NSNLVPRWATWEHFNELDRKGLAMYGQMTAGSWIYIGAQGIVQGTYETFVEMGRQHYA
                     GDLSGKWLLTAGLGGMGGAQPLAAVMAGASCLAIECQPSRIEMRLRTGYLDRSTDNLD
                     EALAWIEDAKSPVSVGLLGNAAELLPRMFRRGIRPDLLTDQTSAHDPINGYL"
     gene            complement(808..1629)
                     /gene="hutG"
                     /locus_tag="PLH31_09050"
     CDS             complement(808..1629)
                     /gene="hutG"
                     /locus_tag="PLH31_09050"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HQR89393.1"
                     /translation="MMSWLQVMRGDAPLIVSLPHTGTEIPPEIEAKLVSPWLARKDAD
                     WWVDRLYDFATDLGATVVRTAISRSVIDVNRDPSGASLYPGQATTELCPTTTFDGEPL
                     YSDGIAPDDAEISRRHAAFFDPYHAALTAEIARLRQGHDRVVLYEAHSIRSLVPRLFD
                     GPLPQFNLGTNSGGSCDGALTTAVEAACDASGLSRVTNGRFKGGWTTRRYGQPAQGVH
                     AIQMELACRGYMDDPTTPPTPETWPAPHRPDQAAPMRAMLGEILSACIAFARPPA"
     gene            complement(1626..>2672)
                     /locus_tag="PLH31_09055"
     CDS             complement(1626..>2672)
                     /locus_tag="PLH31_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQR89394.1"
                     /translation="DGVRTPAAQALDDAGLTPLVLGPKEGLALLNGTQFSNANALAAL
                     FEAELLFQSALITGALSTEAAKGSDTPFDPRIHRLRRHGGQIETAAALRGLMAGSAIR
                     ASHLKEDERVQDPYCLRCQPQVMGAALDVLKQAATTLATEANGVSDNPLIFPSDVEGG
                     DEALSGGNFHAEPVAFAADMIALAVCEIGSIAERRIAMLVDPALSGLPAFLTPKPGLN
                     SGFMIPQVTAAALVSENKQKAYPASVDSIPTSANQEDHVSMAAHGARRLLAMVESATA
                     VIGIELLAAAQGCDFHAPLKSSEVLEAVRGLVRKQVPHLDDDRHFHPDMEAANRLVRS
                     GAVIAAASDLPGVV"
BASE COUNT          392 a          880 c          986 g          414 t
ORIGIN      
        1 gcagatagcc gttgatcgga tcgtgggcgc tggtctggtc ggtcagcagg tcgggccgga
       61 tgccgcgcct gaacatccgg ggcaggagtt cggcggcgtt gccgagcagg ccgaccgaga
      121 ccgggctctt ggcgtcttcg atccaggcca gggcctcgtc gaggttgtcg gtcgaccggt
      181 ccagatagcc ggtgcgcagc cgcatctcga tccgcgaggg ctggcactcg atggccaggc
      241 acgaggctcc ggccattacc gccgccaggg gctgggcccc gcccatgccg cccaggcccg
      301 ccgtcagcag ccacttgccc gacaggtcgc cggcatagtg ctggcggccc atctcgacga
      361 aggtctcata ggtgccctgc acgatgccct gggcgccgat atagatccac gagccggcgg
      421 tcatctggcc gtacatggcc aggcccttgc gatcgagttc gttgaaatgc tcccaggtcg
      481 cccagcgagg caccaggttc gagttggcga tcagcacacg gggcgcgtcg gtgtgggtgc
      541 ggaacacgcc gactggcttg ccggactgga ccagcagggt ttcgtcatcc tcgagccggc
      601 gcagggtctc gacgatcttg tcgaagcttt cccagtcgcg cgccgcccgg ccgatgccgc
      661 catacaccac aagttcttcg ggccgctcgg cgacgtcggg gtgcaggttg ttcatcagca
      721 tccgcagtgg cgcctcggtc agccaggact tggcggtcag ctgggttcca gtggcggcgc
      781 ggatgacgcg gctgttgtcg atgcgggtca tgctgggggt ctcgcaaagg cgatgcaggc
      841 ggaaaggatc tcgcccagca tcgcgcgcat cggcgcggcc tgatcggggc gatggggggc
      901 gggccaggtt tcgggcgtcg ggggggtggt cggatcgtcc atgtagcccc ggcacgccag
      961 ctccatctgg atggcgtgga cgccttgagc gggctggcca tagcggcgcg tggtccagcc
     1021 gcccttgaag cggccgttgg tgacgcggct gagacccgag gcgtcgcaag cggcctcgac
     1081 ggccgtcgtc agggcgccgt cgcagctgcc gccgctgttg gtgccgaggt tgaactgcgg
     1141 cagcgggccg tcgaacaggc gcggaacgag cgagcggatc gaatgggcct cgtaaagcac
     1201 gacgcggtca tggccttggc gcagccgggc gatctcggcg gtcagggcgg catgataggg
     1261 atcgaagaag gcggcgtgcc ggcgggagat ttcggcgtcg tccggcgcga tgccgtcgct
     1321 atagagcggc tcgccgtcaa acgtcgtggt cgggcaaagc tcggtggtcg cctgacccgg
     1381 atagagcgag gcccccgacg ggtctcggtt gacgtctatg accgagcgcg agatcgccgt
     1441 ccgcaccacc gtcgcgccca ggtcggtcgc gaaatcatag agccggtcca cccaccagtc
     1501 ggcgtccttg cgggccagcc acggcgacac gagcttggcc tcgatctccg gcgggatctc
     1561 ggttccggtg tgcggcaggc tgacgatcag cggggcgtcg ccacgcatga cctggagcca
     1621 ggacatcaca ccacccccgg cagatcactc gccgccgcga tcacagcccc tgagcgcacc
     1681 agccgattgg cggcctccat gtcggggtgg aagtgccggt cgtcgtccag gtggggcacc
     1741 tgcttgcgca ccagcccccg caccgcctcc agcacctcgc tcgacttcag cggcgcatgg
     1801 aagtcgcagc cctgcgccgc cgccagcagt tcgatgccga tcaccgccgt ggcgctctcc
     1861 accatggcca gtagacgccg cgcgccatgg gcggccatcg agacgtggtc ttcctggttg
     1921 gccgaggtgg ggatcgagtc gacgctggcc ggataggcct tctgcttgtt ctccgacacc
     1981 agggcggcgg cggtgacctg cgggatcatg aagcccgagt tgaggcccgg cttgggcgtc
     2041 aggaaggccg gcaggcccga cagggccggg tcgaccagca tggcgatgcg gcgctcggcg
     2101 atcgagccga tctcgcagac ggccagggcg atcatgtcgg ccgcgaaggc caccggctcg
     2161 gcgtgaaaat tgccgccaga cagggcctcg tcgccgccct ccacgtcgct ggggaagatc
     2221 agcggattgt ccgacacgcc gttggcctcg gtggccaggg tggtcgccgc ctgtttcagc
     2281 acgtccagcg ccgcgcccat cacctggggc tggcagcgca ggcaataggg atcctggacc
     2341 cgctcgtcct ccttcagatg cgaggcgcgg atggccgagc cggccatcag gccgcgcagg
     2401 gcggcggccg tctcgatctg gccgccgtgg cggcgcagcc ggtggatgcg gggatcgaac
     2461 ggggtgtccg aacccttggc ggcctcggtc gacagggcgc cggtgatcag ggcggactgg
     2521 aacagcagct cggcctcgaa cagggcggcc agggcgttgg cgttcgagaa ctgggtgccg
     2581 ttcagcaggg ccaggccctc cttgggaccc agcaccagcg gcgtcagacc ggcgtcgtcc
     2641 agggcctggg cggcgggcgt gcggacgccg tc
//
LOCUS       DAOMEO010000367         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4119_length_2669_cov_2.288829, whole genome shotgun sequence.
ACCESSION   DAOMEO010000367 DAOMEO010000000
VERSION     DAOMEO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4119_length_2669_cov_2.288829"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2544)
                     /locus_tag="PLH31_09060"
     CDS             complement(<1..2544)
                     /locus_tag="PLH31_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQR89395.1"
                     /translation="MLDRIIALSIRFRWIVLALVLASAVVGIWSFQRLPIDATPDITN
                     VQVQINTEAPGFSPLEAEQRITFPVETAIAGLPGLDYTRSVSRYGLSQVTVVFKDGTD
                     IYFARQLVNERLQAARAQLPPSLTPELGPISTGLGEIFMYTVEAKPGARRPDGKAYTP
                     EDLRTLQDWVIRPQLRNTPGVTEVNTIGGFERQYHVTPLPERLSAYGLTLADVTDALA
                     KNNSNVGAGYIERYGEQYLVRVPGQASGVEDLRGTIITNRNGAPIRIGDVADVGLGEE
                     LRTGAATENGKEVVLGTVFMLVGENSRTVARAAAQRLEQAAKALPAGVVAEAVYDRTE
                     LVDRAIWTVEKNLLEGALLVIVVLFLLLGNIRAALITAAIIPFSMLLTITGMVQSRVS
                     GNLMSLGALDFGLIVDGAVIIVENCLRRFGEAQHRLGRLLGREERFALTATATGEVIK
                     PSLFGVLIITLVYVPIFALTGVEGKMFHPMAITVVIALTAALVLSLTFVPAAVATFVT
                     GKVEDKESRIMRGARRLYEPALAMALRLRVAFVAGAVALVVIAGFAASRMGSEFVPNL
                     DEGDIAMHALRIPGTSLSQAILMQGALEARIKQIPEVERVVGKIGTAEVATDPMPPSV
                     ADTFIMLKDRKDWPDPRKPRAQLVAELQAAVATIPGNNYEFTQPIQMRFNELLSGVRA
                     DVAVKVFGDDLDQLLEIGKSVGAVVEGIEGAQDVGVEQVTGLPVLQITPDRAALARLG
                     LNVQDLQEVVATSIGGAVVGQVFEGDRRFDIVVRLPEATRSNVDGVGRLRVPLPAALD
                     GSRGFVPLQDVARVETVIGPNQISREDGKRRVVITANVRGRDLGSFVTEV"
     gene            complement(2557..>2669)
                     /locus_tag="PLH31_09065"
     CDS             complement(2557..>2669)
                     /locus_tag="PLH31_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89396.1"
                     /translation="EWTEVLGGLEPGTEYVADGAFLIRADIEKSGASHDH"
BASE COUNT          481 a          869 c          885 g          434 t
ORIGIN      
        1 aacctcagtg acgaacgagc cgaggtcacg gcctcgcaca ttggcggtga tcacgacgcg
       61 gcgcttacca tcctcccggc taatctggtt cggaccgatg accgtctcga ccctggccac
      121 gtcctggagc ggtacgaacc cgcgcgatcc gtccaaggcg gccggtagcg ggacgcgaag
      181 ccggccaaca ccatcgacgt tgctgcgagt ggcctctggc aggcgaacga caatgtcgaa
      241 acgcctgtcg ccctcgaaca cctggcccac gaccgcgccg ccgatcgaag tggcgacgac
      301 ctcctgcaaa tcctgcacgt tcagacccag gcgcgccagg gcggcgcggt cgggggtgat
      361 ctgcaacacc ggcaggccgg tgacctgttc aacgcccacg tcctgggcgc cctcaatgcc
      421 ctcgacaacg gcgccaacac tcttgccgat ttcgaggagc tgatcgagat catcgccaaa
      481 caccttgacc gcgacgtcgg cgcgaacgcc cgacaacagt tcgttgaacc gcatctggat
      541 cggctgggtg aactcataat tgttgccggg gatggtggcg acagcggcct ggagttcggc
      601 cacgagttgg gcgcggggtt tgcggggatc gggccaatcc ttgcgatcct tgagcatgat
      661 gaaggtgtcg gcgacgctgg gcggcatcgg gtcggtagcg acctcggcgg tgccgatctt
      721 gccgaccacg cgctcaacct ccggtatctg cttgatccgc gcctcaaggg ctccctgcat
      781 cagaatggcc tgtgacaggc tggtccctgg aatgcgcagg gcgtgcatgg cgatatcgcc
      841 ctcgtcgaga ttgggcacga actccgaacc catccggctg gcggcgaagc cggcgatcac
      901 caccagggcg accgcgccgg ccacgaaagc gactcgcaga cgcaaggcca tggccagagc
      961 cggttcatag agacggcgcg cgccgcgcat gatgcggctt tccttgtcct cgaccttgcc
     1021 agtgacgaag gtcgccacgg cggcggggac gaaggtcagg gacagcacca gggcggcggt
     1081 cagggcgatg accacggtga tcgccatcgg gtggaacatc ttcccctcca ccccggtcag
     1141 agcgaagatc ggcacataga caagggtgat gataagcacg ccaaacagcg agggtttgat
     1201 cacctcacca gtcgccgtgg cggtcagggc gaagcgctcc tccctgccaa gcaggcgacc
     1261 cagccggtgc tgggcctcgc caaaccgacg cagacagttt tcgacgatga tcactgcgcc
     1321 gtcgacgata agaccaaagt ccagcgcgcc caggctcatc agattgcccg acacccggct
     1381 ctggaccatg ccggtgatgg tcagcagcat cgagaacggg atgatcgccg ccgtgatcag
     1441 cgccgcccgg atattgccca gcagcaggaa cagcaccacg atcaccagta acgcgccttc
     1501 gagcaggttc ttctcgacgg tccagatggc gcgatcgacc agttcggtgc ggtcatagac
     1561 cgcctcggcg acaacgccgg ccggcagggc cttggcggct tgctccaggc gctgcgccgc
     1621 cgcgcgggcg acggtgcggc tgttctcgcc caccagcatg aagacggtgc ccagcaccac
     1681 ctccttgccg ttctcggtgg cggccccggt gcgaagctcc tcgcccaggc cgacatcggc
     1741 gacgtcgccg atgcggatcg gcgcgccatt gcgattggtg atgatcgtgc cgcgcaggtc
     1801 ctcgacgccg ctggcctggc ccggaacccg aaccagatac tgctcgccat agcgctcgat
     1861 atagccggcc ccgacattgc tgttgttctt ggccagggcg tcggtgacgt cggccagggt
     1921 caggccgtag gccgacaagc gctcgggcaa gggcgtgacg tgatactgcc gctcaaagcc
     1981 cccgatggtg ttgacctcgg tgacgccggg cgtgttgcgc agctggggcc ggatcaccca
     2041 gtcctgcaag gtgcgcaggt cttcgggcgt gtaggccttg ccgtcggggc ggcgggcgcc
     2101 tggcttggcc tcaaccgtgt acatgaagat ttcgccaagg cccgtcgaga tcggccccag
     2161 ctccggcgtc aggctgggag ggagttgagc gcgggcggct tgcagccgtt cattgaccag
     2221 ctgcctggca aaatagatgt ctgtcccgtc cttgaagacg accgtgacct ggctcaggcc
     2281 ataacgtgag accgagcggg tatagtccag ccccggtaag ccggcgatgg ccgtctcgac
     2341 cggaaaggtg atgcgttgct cggcctccag cggcgagaag cctggagcct cggtgttgat
     2401 ctgaacctgg acattggtga tgtcgggggt ggcgtcgatc ggcaagcgct ggaagctcca
     2461 gataccgacg accgccgagg cgaggacgag cgccagcacg atccaccgaa agcggatcga
     2521 cagcgcgatg atgcggtcaa gcatggctgc gcgctcctag tggtcgtggc tcgcgccgga
     2581 cttttcgatg tccgcgcgaa tcaggaaggc gccgtcggcg acatattcgg ttccgggctc
     2641 gagcccgccc aggacctcgg tccattcgg
//
LOCUS       DAOMEO010000368         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4128_length_2666_cov_2.016469, whole genome shotgun sequence.
ACCESSION   DAOMEO010000368 DAOMEO010000000
VERSION     DAOMEO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4128_length_2666_cov_2.016469"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLH31_09070"
     CDS             <1..316
                     /locus_tag="PLH31_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR89397.1"
                     /translation="ELSTAFDAVIGADLSPAAKPDPRHVLAAIAAVGGDPGRAVMVGD
                     SINDALAANRAKIPALLVSFGYTEEPVETLGGDLVIHSFLDVPKACIDLLASCPRPNT
                     GL"
     gene            387..461
                     /locus_tag="PLH31_09075"
     tRNA            387..461
                     /locus_tag="PLH31_09075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:419..421,aa:Val,seq:cac)
     gene            551..>911
                     /locus_tag="PLH31_09080"
     CDS             551..>911
                     /locus_tag="PLH31_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR89398.1"
                     /translation="MCDDDIHPGLIHDPTVSRRAFGLMTVIADGIGGAAHAGAPVVEK
                     DVEIRTPDGVADAALYYPEGKGSWPAVIVWPDVVSLRPAFREMGRRLAASGYVVLVPN
                     LYYRVKKAPVVESGFNFG"
     assembly_gap    912..921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <922..1417
                     /locus_tag="PLH31_09085"
     CDS             <922..1417
                     /locus_tag="PLH31_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR89399.1"
                     /translation="DRAKITPMRATVTPEGTDRDATAYIAFLDALPQTNKTKKVGVQG
                     YCMGGPLAFRTAGISPSRVAAVASFHGGGLVTDAPTSPHLTLPVSRAEYLICVADNDD
                     KKDPADKEKLKVALDAAKRPNKIEVYEGAAHGWTVKGSQVYSEPAAEKAWAELLALYK
                     RTLG"
     gene            complement(1459..1950)
                     /locus_tag="PLH31_09090"
     CDS             complement(1459..1950)
                     /locus_tag="PLH31_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQR89400.1"
                     /translation="MTDTTEIEAKFWKALRSDMTVMLGLAGVDEGHSQPMTAQFEGKE
                     DRGPIWFFAARNSDLVTALHQSHRGVFDFTSKGHDLFAAVHGELTADNDRAMIDTLWN
                     PFVAAWFPGGKDDPNLQLLRFEPERAQIWLNGSSLLAGVKMLLGADPKKDYADKVAEV
                     ALK"
     gene            2149..>2666
                     /locus_tag="PLH31_09095"
     CDS             2149..>2666
                     /locus_tag="PLH31_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89401.1"
                     /translation="MTARTIKALRWWMIGLIMLGAVINYLTRSTLGVAAPTVLADLNI
                     SEKEYGWITGAFQLGVMMQPLCGYVLDVIGLKTGFAIFATAWSLITMAHGLASNWPML
                     AGLRGLLGLAEGSANPAGMKAVSEWFPARERGHAGGFYNIGASFGSMLAPPLVAWATI
                     SWNWRAAFVLTGV"
BASE COUNT          442 a          894 c          845 g          475 t
ORIGIN      
        1 cgagttatcc acagccttcg acgccgtgat cggggccgac ctgtccccag ccgccaagcc
       61 tgacccgcgc cacgtcctgg ccgccatcgc ggcggtgggc ggcgatccgg gccgggcggt
      121 gatggtgggc gacagcatca atgacgccct cgccgccaac cgcgccaaga tcccagccct
      181 gctggtcagc ttcggctata ccgaggaacc ggtagagacc ctgggcggcg atctggtcat
      241 ccacagcttt ttggatgtgc caaaggcctg tatcgacctt ctggcctctt gcccccggcc
      301 aaacaccggg ctataggccc gtccctctcg cggatcggtc ttccggcccg gcgagaacgg
      361 ttctccggaa ccgcccgaaa cggtatggtc gcgtagctca gcgggagagc gcctgcttca
      421 cacgcaggat gtcacaggtt caatccctgt cgcgaccacc attcccctcg acaccgacat
      481 cagccccttc cctcgccgcg ctttcgcggc gacagtcccg gcctgttcaa ttgcagtgcg
      541 aggatcggcc atgtgtgacg acgacatcca ccccggcctg atccacgatc ccaccgtgtc
      601 ccgccgcgct tttggtctga tgaccgtgat cgccgacggg atcggcggcg cggcccacgc
      661 aggcgcgccg gtcgtggaga aggacgtcga gatcaggacg ccggacggcg tggcggacgc
      721 ggccctgtac tatcccgaag gcaagggctc gtggcccgcc gtgatcgtct ggcccgacgt
      781 ggtcagcctg cggccggcct tccgcgaaat gggccgccgg ctagcggcct ccggctatgt
      841 ggtgctggtt cccaaccttt attaccgggt caagaaggcg ccggtggtcg agagcggctt
      901 caactttggc annnnnnnnn ncgatcgcgc caagatcacg ccgatgcggg ccaccgtcac
      961 gccagaaggc acagacaggg acgccaccgc ctatatcgcc ttcctcgacg ccctgcccca
     1021 gaccaacaag accaagaagg tcggcgtcca gggctattgt atgggcggtc cgctggcgtt
     1081 ccgcacggcc ggcatctcgc cgtcccgcgt cgccgccgtg gccagcttcc atggcggcgg
     1141 tctggtgacg gacgccccga ccagtcccca cctgaccttg ccggtcagcc gcgccgaata
     1201 cctgatctgc gtcgccgaca acgacgacaa gaaggatccg gccgacaagg aaaagctgaa
     1261 ggtcgcgctc gacgccgcca aacggccaaa caagatcgag gtctatgaag gcgccgccca
     1321 cggttggacc gtgaagggca gccaggtcta tagcgagccc gccgccgaaa aggcctgggc
     1381 cgaattgctg gcgctgtaca agcgcacact gggctgaagg gttggccgcg ccgataacgg
     1441 cgcggccaac ctgtctgcct atttcaaagc cacttcggcg accttgtcgg catagtcctt
     1501 cttgggatca gcgccgagca gcatcttcac gcccgccagc aggctggagc cgttgagcca
     1561 gatctgcgcc cgttcgggct cgaagcgcag cagctggagg ttgggatcgt ccttgccgcc
     1621 cggaaaccac gcggccacga acggattcca cagcgtgtcg atcatggccc ggtcattgtc
     1681 tgcggtcagt tcgccatgaa ccgccgcgaa caggtcgtgg cccttcgagg tgaaatcgaa
     1741 aacgccccga tggctttgat gcagcgcggt aaccagatcg ctgttgcgcg cggcgaagaa
     1801 ccagatcggt ccgcgatctt ccttgccttc gaactgggcg gtcatgggct ggctatggcc
     1861 ttcgtccacg ccggccagtc ccagcatcac ggtcatgtcg gatcgtagag ccttccagaa
     1921 cttggcttca atttcggtgg tgtcggtcat cgttttctcc agagttgctc tggcaacgac
     1981 agcgagcacg cgcttgttcc acgccttggg cgccatcgag gtgatctgac cttcatttgt
     2041 cttggtctga cctagacagc gctccgccga cgcgccacct tgcgcagcgt cgagatggga
     2101 ccggtaactt ccgaacaatc cgtccggctt cggggaggct gccgtccaat gaccgcacgt
     2161 acgatcaagg ccctgcgttg gtggatgatc ggcctgatca tgctgggcgc ggtcatcaac
     2221 tatctgaccc gttccaccct gggcgtcgcc gcgcctacgg tgctggccga cctgaacatc
     2281 agcgaaaagg aatatggctg gatcacgggc gcctttcagc tgggcgtgat gatgcagccg
     2341 ctgtgcggct atgtgctgga cgtcatcggc ctgaagaccg gcttcgcgat cttcgccacc
     2401 gcctggtcgc tgatcaccat ggcccacggc ctggcgtcca actggccgat gctggcgggc
     2461 ctgcggggcc tgctggggct cgcggagggc tcggccaatc cggcgggcat gaaggcggtg
     2521 tcggagtggt tccccgccag ggagcgcggc cacgccggcg ggttctataa tatcggggcc
     2581 tcgttcggct cgatgctggc gccgccgctg gtggcctggg cgaccatctc gtggaactgg
     2641 cgcgccgcct tcgtgctgac cggcgt
//
LOCUS       DAOMEO010000369         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4129_length_2665_cov_3.067433, whole genome shotgun sequence.
ACCESSION   DAOMEO010000369 DAOMEO010000000
VERSION     DAOMEO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4129_length_2665_cov_3.067433"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLH31_09100"
     CDS             <1..462
                     /locus_tag="PLH31_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQR89402.1"
                     /translation="NAILAEPAFAGDRVVLIEDTAELQCSAADQIQMLTKRTEPAVTM
                     TDLVRDTLRLRPDRIVIGEVRDGSALDLLKAWNTGHPGGLATLHANSAAEGLSRLEDL
                     IGEVTQRIPHRAIGQAIDLVIHIARTPGGRRVSEIMRVTGWGEGGYDLRAA"
     gene            560..1144
                     /locus_tag="PLH31_09105"
     CDS             560..1144
                     /locus_tag="PLH31_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQR89403.1"
                     /translation="MEQQQHKMQMGWGRFAAMIVVSTIIMFFLMYQLVYSVEHALFSL
                     TRLVSSLVMGSVMTAVMLAFMWKMYQPNAAKMAVLVGAVIGGIVLLAVNRSQALIGDE
                     DFMKAMIPHHSIAINNARKADLRDPRVRYLADRITRDQVKEIAEMKMLLADIDKNGRR
                     ANAPLAAGPDTLDPKSAQEAKDLVSGKLLTDKPL"
     gene            complement(1311..1796)
                     /locus_tag="PLH31_09110"
     CDS             complement(1311..1796)
                     /locus_tag="PLH31_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR89404.1"
                     /translation="MSDRQKTPSYGRALLQLGAMAGGFVIAISLGHHAFVESLKPPPA
                     PPAPAAAPPSAVSGGGFTLTSTSIALPDDDPPYPAGPGADLMNGNCTACHSASMALTQ
                     PALSKAQWTSTVEKMRDTYKAAIAEKDIRAIVEYLTKVSAQAKGDGLSAPTSDAGGAS
                     G"
     gene            complement(1793..>2665)
                     /locus_tag="PLH31_09115"
     CDS             complement(1793..>2665)
                     /locus_tag="PLH31_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR89405.1"
                     /translation="SLADILAMPRVQMAAVNQCSGNSRGLFQPRVPGAQWAHGAMGNA
                     KWLGVSLRRVLDLAGVQPGAVAVRFNGLDQPVVDGAPDFSKSLSIDHARDGEVMIAFA
                     MNGAQLPLLNGFPIRLIVPGWFSTYWVKMLSDIEVLGAPDDSYWMAKAYKIPDTPLAN
                     VAPGAKDFPTVPINRMVPRSWITSLADGTSVAYQPHLPVGGIAMGGDAGVARVDLSSD
                     GGRSWAPARLGPDEGKYSFRRFDGMVAVAGRGPVALMARCTNTAGVSQALTPNWNPGG
                     FMRACVETTHVVLS"
BASE COUNT          452 a          842 c          919 g          452 t
ORIGIN      
        1 aacgccattc tggctgagcc ggcctttgcc ggcgacaggg tggtgctgat cgaggatacc
       61 gccgagctgc agtgttcggc ggccgatcag atccagatgc tgaccaagcg caccgagccg
      121 gcggtgacca tgaccgacct ggtgcgtgac accttgcgcc ttcggcccga ccgcatcgtg
      181 atcggggagg tccgggacgg ctcggcgctt gatctgttga aggcttggaa caccggccac
      241 cctggcgggc tggccacgct gcacgccaac agcgcggccg agggcctaag ccggctggag
      301 gatctgatcg gtgaggtgac ccagcgcatc ccgcaccggg ccattgggca ggccatcgat
      361 ctggtgatcc acatagcgcg cacaccgggc gggcggcggg tgtcggaaat catgcgggtc
      421 acgggctggg gagagggcgg gtacgacttg cgggccgcct gaggctgcat ccgacggcca
      481 tcggtgggcc cgccttggcg cactggtgaa ccctgcctct cttggcgggt taccgatctt
      541 aggaccggag gatggctcga tggaacagca acaacataag atgcagatgg gctggggtcg
      601 gttcgccgcc atgatcgtcg tgtcgacgat catcatgttc ttcctgatgt atcagctggt
      661 ctatagcgtc gagcacgcgc tcttcagcct gacacgactg gtctcatcct tggtgatggg
      721 ctccgtgatg acggcggtga tgctggcctt catgtggaag atgtaccagc ccaacgccgc
      781 caagatggcc gtgcttgtcg gcgccgtcat cggcgggatc gtcctgctcg ccgtcaatcg
      841 gagtcaggcg ctgatcggcg acgaggattt catgaaggcc atgatccccc accattccat
      901 cgccatcaac aatgcccgca aggccgatct ccgtgacccg cgggtgcgct atctcgccga
      961 ccggatcacg cgcgatcagg taaaggagat cgccgagatg aagatgctgc tggcggacat
     1021 cgacaagaac ggccgccgcg ccaacgcgcc gctggccgca gggcccgata cgctcgatcc
     1081 gaagagcgcg caggaggcca aggatctcgt cagcggtaag ctgctgacgg ataagcctct
     1141 ctgaccggtt cgacgccagc ggcacgagcg ggctgtcggt cctgtcccgt cggttgaaac
     1201 tcttttggcc ggagctcgcc aagacggtcg ttgttgagct ttcaggcgac cagaaagcca
     1261 ggataccggc ctcaaagccg cgccgtacaa agaccatttg aagcgttggc ctacccgctt
     1321 gctccgccag cgtccgacgt cggcgcagag aggccgtcgc ctttggcctg cgcactgacc
     1381 ttggtcagat attcgacgat cgctctgatg tccttttccg cgatcgccgc cttgtacgtg
     1441 tcgcgcatct tctcgacggt cgaggtccac tgcgccttcg acaacgcggg ctgggtcagg
     1501 gccatgctgg ccgaatgaca tgcggtgcag ttgccgttca tcaggtcagc gcccggcccg
     1561 gcagggtagg gcggatcgtc gtcgggcaag gcgatgcttg tcgaggtcag cgtgaagccg
     1621 ccccctgaca ctgcggatgg cggcgccgcg gctggagccg gcggtgcggg cggcggcttc
     1681 agactttcca cgaaggcatg gtggccaagg gagatcgcaa tgacgaagcc tcccgccatg
     1741 gcgccaagtt gcagcagcgc gcggccgtag gacggcgtct tctgccggtc gctcatgaca
     1801 gcaccacgtg ggtcgtctcg acacaggcgc gcatgaagcc gcctggattc cagttcggcg
     1861 tcagggcctg gctgacgccg gctgtattcg tgcaccgggc cattagagcg acggggcccc
     1921 ttccggcgac ggcgaccatc ccgtcgaagc ggcggaagct gtacttgccc tcgtcggggc
     1981 ccaggcgagc gggcgcccag gagcggccgc cgtcgctgga aagatcgacc ctggcgaccc
     2041 cagcgtcgcc gcccatggcg atgccgccga ccgggaggtg gggttggtag gcgacgctgg
     2101 tgccgtcagc aaggctggtg atccaggacc gcgggaccat ccggttgatc ggaacggtcg
     2161 ggaagtcctt ggcgcccggc gccacattgg ccaatggcgt gtccgggatc ttgtaggcct
     2221 tggccatcca gtaactgtcg tccggggcgc cgagcacctc gatgtcgctg agcatcttga
     2281 cccagtaggt cgagaaccac cccgggacga tcagtcggat cgggaagccg ttgagcagcg
     2341 gcaactgcgc gccgttcatc gcgaaggcga tcatcacctc gccgtcgcgg gcatggtcta
     2401 tcgagagcga tttcgagaag tccggggcgc catcgacgac cggctggtca aggccgttga
     2461 agcgcaccgc gaccgcgccg ggctggacgc cggccaggtc gaggacacgc cgcaagctta
     2521 cgcccagcca cttggcgttg cccatggcgc cgtgcgccca ctgggcgccg ggaacccggg
     2581 gctggaacag accgcgggaa ttgcccgagc actggttgac ggcggccatc tgcacgcgcg
     2641 gcatggccag gatgtcggcg aggga
//
LOCUS       DAOMEO010000370         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4134_length_2662_cov_2.649022, whole genome shotgun sequence.
ACCESSION   DAOMEO010000370 DAOMEO010000000
VERSION     DAOMEO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_2662_cov_2.649022"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..2377
                     /gene="polA"
                     /locus_tag="PLH31_09120"
                     /pseudo
     CDS             205..2377
                     /gene="polA"
                     /locus_tag="PLH31_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477990.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
     assembly_gap    2378..2387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2388..>2662
                     /locus_tag="PLH31_09125"
     CDS             <2388..>2662
                     /locus_tag="PLH31_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQR89406.1"
                     /translation="SEMFGVPIEGMPSEVRRRAKAINFGIVYGISAFGLANQLGIGQG
                     EAGAYIKTYFERFPGIQAYMDATKAFVREHGYVTTIFGRKINIPDIHA"
BASE COUNT          455 a          910 c          865 g          422 t
ORIGIN      
        1 accagaatga caggaacgcg accgagcgtt tgacgccgct gcatccgccc aatcgggcgc
       61 ccgccaagcc ttcccgccgc acggtcgccg gcctatgttc cggccatgac cgacaccgcc
      121 gcgcccgccg attccgcccc tgcctcgacc ctcacccagg acggccccgc cgtgcggctg
      181 ttcctggtgg atggctcggc ctatctgttt cgcgcctatc acgccctgcc gcccctgacc
      241 cgcaagagcg atggcctgcc ggtgggggca gtgcagggtt tctgcaacat gctgtggaag
      301 ctgctgcgcg acatgcaggg cgatgcgccc acccatctgg cggtgatctg ggaccactcg
      361 gaaaagacgt tccgcaacca gctgtacgac aagtacaagg cccaccgccc gccgccgccc
      421 gaggacctga tcccgcagtt cccgctggtg cgcgaggcga ccctggcgtt tggcgtgccg
      481 gccatcgagt tgcccggcta tgaggccgat gacctgatcg ccgcctatgc ctgccgcgtg
      541 cgcgagctgg gcggcgagac cgtgatcgtc tcgtccgaca aggacctgat gcagctggtc
      601 ggcgacgggg tttcgatgtt cgacccgatg aagggcctgc gcatcgaccg cgagcaggtg
      661 ttcgagaagt tcggcgtcta tccggaaaag gtggtcgatg ttcaggccct gtgcggcgac
      721 agcgtcgaca atgtgcctgg cgcgcccggc atcggcatca agaccgccgc ccagctgatc
      781 aacgagtatg gcgatctgga cacgctgctg gcgcgggccg gcgagatcaa gcagcccaag
      841 cgccgcgaga cgctcattga gttcgccgac cagatccgcc tgtcgcgggc cctggtgcag
      901 ctggactgcg acacgcccct gcccgagccg atcgatgatt tgaccgtgcg cgagcccgac
      961 aaggtggtgc tggccgactt cctggaactg atggagttcc gctccctggc ccgccgggtg
     1021 ggcgacggca acgcctcggc cgcgccgcgc acgcttgacc gcccgccggg ccccgcgccg
     1081 agtgcgccgg tggccagcat ctcctatgcg agcgccgccg cccgcgccgc cgccgcgccg
     1141 gtcgccgagc cggccgccat cgatcacgcg gcctatgtgc gcatccagga tatcgagacc
     1201 ctgaacgcct gggtcgccaa ggccacggcc aaggggatcg tagcgttcga caccgagacc
     1261 gacgccctgt cctcggcgac ggccggcctg tgcggcgtgt cgctggcgat caatccgggc
     1321 gaggcctgct acatcccggt cggccattgc gagaaggacg gcctcgccct ggaggccgcc
     1381 gccgatctgg tgcagatccc gctggacgaa gcgatagcgg ccctgaagcc gctgctggaa
     1441 gatccggcgg tgctgaagat cgcccagaac gccaagtacg acatcgccgt cctggcccgg
     1501 catggcatcg acgtggcgcc gatcgaagac accatgctga tcagctatgt gctggaggcc
     1561 ggcttgcacg gtcacggcat ggacgagctg tcagagctgc atctgggcca caagccgatc
     1621 ccgttcaagc aggtggcggg gacgggcaag gcgcagatca gcttcaagca tgtgggtctg
     1681 accgaggcca cggcctatgc cgccgaggac gccgacgtca ccctgcggct ctacaacatc
     1741 ctgaaacccc gcctggcgcg cgaggggctg ctgacggtct atgagacgct cgagaggccc
     1801 ttgccgccgg tgctggcggc gatggagaac gaaggcattc gggtcgaccc cgacaccctg
     1861 cgccggcttt ccaacgagtt ctcgatgcgg atggccgact tcgaggccca ggcccaggcc
     1921 ctggtcgggc ggccgttcaa tctgggcagt cccaagcaga tcggcgatgt gctgtttggc
     1981 gagatggccc tgaagggcgg caagaagacc gccaccggcc agtggtcgac cgacagcgat
     2041 gtgctggaga gcttggccct tgagcatgaa ttgccacgcg tgctgctgga ctggcgccag
     2101 ctgtccaagc ttaagggcac ctataccgac aacctgatcg ccgccatcgc gccgggcagc
     2161 gagaaccgca tccatacctc ctacgccctg gccgcgacca cgacggggcg actgtcgtca
     2221 tccgatccga acctgcagaa catcccgatc cgtaccgagg aaggccgcaa gatccgcaag
     2281 gctttcgttg ccgcgccggg caagctcttg atcagcgccg actacagcca gatcgagctg
     2341 cgcctgctgg cccatatcgg ggacatcccg cagttgannn nnnnnnntcg gaaatgtttg
     2401 gcgtgccgat cgaggggatg cccagcgagg tgcgccgccg ggccaaggcg atcaatttcg
     2461 gtatcgtcta tggcatctcg gcgtttggtc tggccaacca gctgggcatc ggccagggcg
     2521 aggccggggc ctatatcaag acctatttcg agcgcttccc cggcatccag gcctatatgg
     2581 acgccaccaa ggccttcgtg cgcgagcatg gctatgtgac cacgatcttc ggccgcaaga
     2641 tcaacattcc cgacatccac gc
//
LOCUS       DAOMEO010000371         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4140_length_2658_cov_2.157511, whole genome shotgun sequence.
ACCESSION   DAOMEO010000371 DAOMEO010000000
VERSION     DAOMEO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4140_length_2658_cov_2.157511"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1442)
                     /locus_tag="PLH31_09130"
     CDS             complement(297..1442)
                     /locus_tag="PLH31_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HQR89407.1"
                     /translation="MRDFKGMKRQRGRNNRGGGNTGGKPQQHNANRAFDSNGPEGVKV
                     RGAAQGVYEKYQQLARDATSSGDRVLAENYLQHAEHYFRVLRAIQPNRPVSDIIGKDA
                     YSAYEIDFEAEPEEAPEVAETPAAENNDGGDGEGQQGDRPRDRFENRNRDDRPRDDRP
                     RDDRPRDGQNRDRFDNQGQGQGQGQGRRDRWRDRDDRPRDDRPRDDRPRDERPRDERP
                     RDDRPRDDRPRDDRPREDRFREDRPRDDRPRDDRPREDRFREERPAVAAEAPTEQPQA
                     ETRRERAPRRERAPRDRDRDPMAVIEPQASPLSEVSASEGSPVLRGQDGAFSHAPAFL
                     GRPAAAAETPVPAAADGEEIKKPRRRRAPRSFEGGEGAPAAPESEEA"
     gene            complement(1687..>2270)
                     /gene="prmC"
                     /locus_tag="PLH31_09135"
     CDS             complement(1687..>2270)
                     /gene="prmC"
                     /locus_tag="PLH31_09135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQR89408.1"
                     /translation="PETEVIVDEVLKAFPETMSFNMLDLGVGSGTILLAVLAERPAAR
                     GLGVDVSDEALAVARENAASLDLNNRAALLRGDWTSGLGDEGFDLVVSNPPYIASHII
                     ETLEPEVRDHEPRLALDGGPDGLNAYRELAPEILRVLKPGGMFAVEIGYDQSADVEAL
                     FRAAGASNVRTIKDLSTHDRVVTGVKNPLETPA"
     assembly_gap    2271..2280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2281..2560)
                     /locus_tag="PLH31_09140"
     CDS             complement(<2281..2560)
                     /locus_tag="PLH31_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HQR89409.1"
                     /translation="MTLTLVKAWTAAKERLKDAGIDQPAIDARLMLEVAADVTRTEIV
                     TDPYRMLTAQQWATLDDYLTRRARREPVSHIIGRKGFWKILLQVNKNVL"
BASE COUNT          385 a          850 c          913 g          500 t
ORIGIN      
        1 caatcacgac gatggcgtct acggcggtgt cgacaatggc gcgataacgc gcctcgacca
       61 tgacttcagc cctgggattg ctttggccgc gcgtctcgtg ggttccagtc atgtcattcc
      121 ctaggcatct aggagaacgt atcgccgaag tcgaagatcg actagcccac gcaagcttaa
      181 caatggcacc cagtcgtgca gagccttctc tgggatcagc aagactgaag accggtggcc
      241 ccaaagaaaa aggagccgct ttcacggctc cttttctgga agaccctcga ggctgactaa
      301 gcctcttcgg actccggcgc ggcgggcgcg ccttcgccac cctcgaagct gcgcggggcg
      361 cggcgacgac ggggcttctt gatctcttca ccatccgctg cggccgggac tggcgtttcg
      421 gcggcagccg cggggcgacc caggaaggcc ggggcgtggc taaaggcgcc atcctgaccg
      481 cgcagcacgg gcgaaccctc gctggccgaa acttcgctca gcggcgaggc ctgcggctcg
      541 atcacggcca tcggatcgcg gtcacgatca cgcggcgcgc gttcgcggcg cggcgcccgt
      601 tcgcggcgag tttcggcctg gggctgttcg gtgggcgcct cggcggcgac ggccggacgt
      661 tcctcgcgga aacggtcctc gcgggggcgg tcatcccggg gacggtcgtc gcggggacgg
      721 tcttcgcgga aacggtcttc gcgggggcga tcgtcgcgcg ggcgatcatc ccggggacgg
      781 tcatcgcgcg ggcgctcgtc tctggggcgc tcgtctctgg gccgatcatc gcggggacgg
      841 tcatccctgg gacggtcgtc acggtcgcgc cagcgatcgc ggcgcccctg accctggccc
      901 tggccctggc cctggttatc gaagcggtcg cggttctgac cgtcgcgcgg gcgatcgtca
      961 cggggacggt catcacgcgg gcggtcgtcg cggttgcgat tctcgaagcg gtcgcgggga
     1021 cggtcgccct gctggccttc gccatcgccg ccgtcattgt tctcggcggc gggcgtttcg
     1081 gcgacttcgg gagcctcttc aggctcggcc tcgaagtcga tctcgtaggc cgaataggcg
     1141 tccttgccga tgatgtcgct gacggggcga ttcggctgga tggcgcgcag gacgcggaaa
     1201 tagtgctcgg cgtgctgcag gtaattctcg gccagaaccc ggtcgccgga cgacgtcgcg
     1261 tcccgcgcca gctgctggta cttttcgtaa acgccctggg ccgcgccgcg caccttcacg
     1321 ccttcggggc cgttcgaatc gaaggcccgg ttggcgttgt gctgctgagg cttgccgccg
     1381 gtattgccgc cgccgcgatt gttgcggccg cgctggcgct tcatgccctt gaaatctctc
     1441 atgttctctc tgaacctgtc cggaagtcag tctgagctga ggcatccggc cttgttggtc
     1501 tctagacggg gtcgaacccc tggaaagttc tagagacact ggtttccgta gagcccccgt
     1561 cctggtcctg gcgcggcgac cggctttgga ccgtgcgcgc ctgccggagg agctttgcga
     1621 acgtcatcga cgcccgcttt tcccgtcacc cggcattttg gaaggagacc ccttccgtct
     1681 agcctctcac gcaggggttt ccaagggatt tttcaccccg gtcacgacgc ggtcatgggt
     1741 cgacaggtcc ttgatggtcc ggacgttgct ggcgccggcc gcccggaaca gcgcttcgac
     1801 gtcggccgac tggtcgtagc cgatctccac ggcgaacatg ccgccgggct tcagaacgcg
     1861 caggatctcg ggcgccagct cgcgataggc gttcaggcca tcagggccgc catcgagcgc
     1921 caggcgcggt tcgtgatcgc gaacctccgg ctccagggtc tcgatgatgt ggctggcgat
     1981 atagggcggg ttggagacca ccaggtcgaa gccttcgtcg cccaggcccg atgtccagtc
     2041 gccgcgcaac agggcggcgc ggttattgag gtcgaggctg gcggcgttct ctcgcgcgac
     2101 ggccagggct tcatccgaga catcgacacc caggcccctg gcggccggcc gttcggccag
     2161 gaccgccagc aggatcgtgc ccgagccgac gcccaggtcc agcatgttga agctcatggt
     2221 ctccgggaag gccttgagca cctcgtcgac gatgacctcg gtctcgggac nnnnnnnnnn
     2281 tcaggacgtt cttgttcacc tgcagcagga tcttccagaa gcccttgcgg ccgatgatgt
     2341 ggctgaccgg ttcgcggcgc gcgcggcggg tcagatagtc gtccagcgtc gcccactgct
     2401 gcgcggtcag catgcggtag gggtcggtga cgatctcggt gcgggtgacg tcggcggcca
     2461 cctccagcat caggcgcgca tcaatggcgg gctgatcgat accggcgtcc ttcagacgct
     2521 ccttggcggc ggtccaggct ttgacgaggg tcagggtcat gggcggtcac atagcgcttt
     2581 gggacgccga gcgcgagatt gaggttggta ccagtttttg gtattttagg ctcaggaggc
     2641 cgccatgaac aagaatac
//
LOCUS       DAOMEO010000372         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4146_length_2654_cov_2.266256, whole genome shotgun sequence.
ACCESSION   DAOMEO010000372 DAOMEO010000000
VERSION     DAOMEO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4146_length_2654_cov_2.266256"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLH31_09145"
     CDS             complement(<1..889)
                     /locus_tag="PLH31_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR89410.1"
                     /translation="MAEDPKDWDIATKLIRGGLARSQHMETAEALYLTQGFTYDSAEG
                     ADRRFAGDDPGFVYSRFGNPTVKMFEDRLALLEGAEVCRAQATGMASIHMALMGLVRA
                     GDHVVAGRALFGSCRWIISEWLPRFGVETTFVDATDPKAWEAAMRPNTKAVLIETPSN
                     PVLEITDIRAVSEIAHAVGAKVIVDNVFATPIFQQPLKLGADIVVYSATKHIDGQGRV
                     LGGAILTSQAINDEFYRDALRHTGPSLSPFNAWVLVKGLETLDLRVRRQADTALALAN
                     VIEPRHGRPSGHDQPPLEVF"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1063..2082
                     /locus_tag="PLH31_09150"
     CDS             1063..2082
                     /locus_tag="PLH31_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HQR89411.1"
                     /translation="MTDAHSAGILPCQNIEDLIAQEAITSQTPFDVDQVQPASLDLRL
                     GARAWRVRASFLPGLGRKVTERLKDVAMHELDLTKGAVLEKGCVYIAELQEHLALPLN
                     ISARGNPKSSTGRVDVFVRLLTNHSRAFDDVDAGYHGPLYMEIAPQTFSVLVRTGTRL
                     NQLRLKRGEPAKLSTRSVGVDLSPGIVGFRGRRHAGVVDLDHEDGHDPRDFWEPLETR
                     HGELLLDPGEFYILASKDDIEIPVMEAAEMTPIDPSVGEFRVHYAGFFDPGFGTEEAA
                     AVGSKGVLEVRSHETPFLLEDGQTVARLVYEPLTSRPARLYGEGGSHYQRQGLKLSKH
                     FKAWT"
     assembly_gap    2101..2110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2341..2640
                     /locus_tag="PLH31_09155"
     CDS             2341..2640
                     /locus_tag="PLH31_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89412.1"
                     /translation="MARLSSDPFSILQTRFRAWETCIRIPGCDKAIWRQDAAGRVIRW
                     TDFGDRFSRYGWEMRSCPSDTALGRALKRHKVEAIHWRGVLGDDARHAFELPKAA"
BASE COUNT          446 a          842 c          900 g          446 t
ORIGIN      
        1 tgaaaacttc cagtggtggt tggtcatggc cacttggtcg cccgtgacgg ggttcgatca
       61 cattggccag ggccagggcg gtgtcggcct gacggcggac gcgcaggtcc agcgtctcca
      121 ggcccttgac gaggacccag gcgttgaacg gcgacagcga tgggccggtg tggcgcaggg
      181 cgtcgcggta gaattcatcg ttgatcgctt ggctggtcag gatcgctccg cccagcaccc
      241 ggccctggcc gtcgatgtgc ttggtggccg aatagaccac gatgtcggcc cccagcttca
      301 gcggctgttg gaagatcggg gtggcgaaga cgttatcgac gatcaccttg gccccgaccg
      361 catgggcgat ctctgacacc gcgcggatgt cggtgatctc aagcacggga ttggacggcg
      421 tctcgatcag cacggccttg gtgttgggcc gcatggccgc ttcccaggcc ttgggatcgg
      481 tggcgtcaac aaaggtggtc tcgacgccaa agcgcggcag ccattcggag atgatccagc
      541 gacacgagcc gaacagggcc cggccggcca cgacgtggtc gccggcccgc accagcccca
      601 tcagcgccat gtggatcgag gccatgcccg tggcctgggc gcggcagact tcggcgccct
      661 ccagcagggc caggcgatcc tcgaacatct tcacggtggg attgccgaac cgcgaataga
      721 caaagccagg atcatcgccg gcaaagcggc ggtcggcgcc ctcggcgctg tcataggtga
      781 agccctgggt caggtagagc gcctcggcgg tctccatatg ctgcgaccgg gccagccccc
      841 cgcggatcag tttggtcgcg atgtcccagt ctttggggtc ctcggccacg gggtaatcct
      901 tcttcggcgc ggaaaaggcc ggcataaggg gtttagcggc gtgcagggtc aaggcgcggc
      961 ggtctgattt caacatcaga attgcttgag gcgagccggg cgnnnnnnnn nnggcgcgct
     1021 gcagccttgc cttccaggcg agctttagcg actgtcgcgc cgatgaccga cgcccattcc
     1081 gcagggattc tgccctgcca aaacatcgaa gacctcatcg cccaggaggc gatcacctca
     1141 cagacgccgt tcgacgtcga tcaggtgcag cccgccagcc tggacctgcg gctgggcgcg
     1201 cgcgcctggc gggtccgcgc ctcgttcctg ccgggcctgg gccgcaaggt gaccgagcgc
     1261 ctgaaagatg tggccatgca cgagctggac ctgaccaagg gcgcggtgct ggaaaagggc
     1321 tgcgtctata tcgccgaact gcaggagcat ctggccctgc cgctgaacat ctcggcgcgc
     1381 ggcaatccca agagctcgac gggccgggta gacgtctttg tgcgcctgct gaccaatcac
     1441 agccgcgcct ttgatgatgt ggacgccggt tatcacggcc cgctctatat ggagatcgcg
     1501 ccccagacct tctcggtgct ggtgcggacg ggcacgcggt tgaaccagct gcgcctgaag
     1561 cgcggcgagc cggccaagct gtcgacccgc agcgtcgggg tggacctgtc gccgggcatc
     1621 gtcggtttcc gggggcgtcg ccacgccggc gtcgtcgatc tggatcacga ggacggccac
     1681 gacccgcgcg acttctggga accgctggaa acccgccatg gcgaactgct gctggatccc
     1741 ggcgagttct acatcctggc ctccaaggac gatatcgaga tcccggtgat ggaagcggcc
     1801 gaaatgaccc cgatcgatcc gtcggtcggc gagttccgcg tccactatgc cggcttcttc
     1861 gatccgggct ttggcaccga ggaggccgcc gccgtcggct ccaagggcgt actggaggtg
     1921 cgcagccacg agacgccgtt cctgctggaa gatggccaga ccgtggcgcg cctggtctat
     1981 gagcccctga cctcgcggcc ggcgcggctg tacggggagg gcgggtcgca ctatcagcgc
     2041 cagggcctga agctgtccaa gcacttcaag gcctggacct gagtcaccct gcagctgggg
     2101 nnnnnnnnnn cgagcgaagc gcatgaggcg agctaggcga ggacgggcgt ttacccctgt
     2161 tgcggcgacg caaccgtgcc gcctggtgca tgaatccagg ccaagcctga ccgtcgcggc
     2221 attgcgtcac accacatgtg gtgggcgaga ttgatcaaga cacaagtgat agattgatgg
     2281 cgtcggagga gtcggcaatg actcaggccc gccgcactgt atttccaggg aggccgcgtc
     2341 atggcccgtc ttagttcgga ccccttctcg atcctccaga cccgcttccg cgcctgggaa
     2401 acctgcatca ggatcccggg ttgcgacaag gcgatctggc gacaggacgc cgccggacgg
     2461 gtgatccgct ggaccgattt cggtgatcgt ttctcgcgct atggctggga aatgcgcagc
     2521 tgtcccagcg atacggccct gggccgcgcg ctcaagcgcc acaaggtcga ggccatccac
     2581 tggcgcgggg tgctgggcga cgacgcccga cacgcattcg agctgcccaa ggccgcctga
     2641 atgacggccg agcc
//
LOCUS       DAOMEO010000373         2650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4158_length_2650_cov_2.432755, whole genome shotgun sequence.
ACCESSION   DAOMEO010000373 DAOMEO010000000
VERSION     DAOMEO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4158_length_2650_cov_2.432755"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLH31_09160"
     CDS             complement(<1..479)
                     /locus_tag="PLH31_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89413.1"
                     /translation="MSGEQRDFEGVRAVVTAGTKGAGRATVERLLQGGASVVTAARSA
                     ANAPAGAHVVEVDLTRPDGAEQLARAAIERLGGVDVLVHVLGGSSSPGGGFAALTDDH
                     WAAELDLNLMTAVRLDRALLPQMLERGRGAVVHVSSIQARLPLPESTTAYAAAKAALT
                     "
     gene            complement(479..805)
                     /locus_tag="PLH31_09165"
     CDS             complement(479..805)
                     /locus_tag="PLH31_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR89414.1"
                     /translation="MSIKLPRPVADYVEANARLDVDGMLAAFAADAVVKDEAREHNGH
                     AEIKPWIQSATIAARAIFTPTSWRETDGAVIVEGTTAGDFPGSPLPFAFRFQLSGGAI
                     SALEIV"
     gene            910..1263
                     /locus_tag="PLH31_09170"
     CDS             910..1263
                     /locus_tag="PLH31_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89415.1"
                     /translation="MAYSADSASLSIQNVIRVLEGRWKLVILFQLFGGRVQRFSDLER
                     AIPGVSQKMLIQQLRQLEADGVVTRIVHPQVPPKVEYHLTDWGQSLCPALDALLIWAE
                     QAPAEQRSRLIPEPL"
     gene            complement(1558..1632)
                     /locus_tag="PLH31_09175"
     tRNA            complement(1558..1632)
                     /locus_tag="PLH31_09175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1598..1600),aa:Thr,seq:ggt)
     gene            1839..2132
                     /locus_tag="PLH31_09180"
     CDS             1839..2132
                     /locus_tag="PLH31_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89416.1"
                     /translation="MRRLEGVAHRDGTRVALVLEISPPYPAPESDEVCCAVRLVGDGG
                     QFDTEHGKAAFGVDGLQAMKRALDLAQVALDLASTTYDLRWRDGQSYDLSAPI"
     gene            2233..>2650
                     /locus_tag="PLH31_09185"
     CDS             2233..>2650
                     /locus_tag="PLH31_09185"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517808.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQR89417.1"
                     /translation="MDRIAIIGGARLHGEIPVSGAKNSAIKLMAASLLTDEPLRLTNM
                     PRLADTRFLGKLLGRLGVSVVESDGPDGQQTVLHAPEITSGFAPYDLVRQMRASFNVL
                     GPLVARTGQAKVSLPGGCTIGARPVDLHLQALEALGA"
BASE COUNT          446 a          891 c          882 g          431 t
ORIGIN      
        1 gtcagggcgg ccttggccgc ggcataggcc gtcgtggact ccggcagcgg caatctcgcc
       61 tggatcgacg agacgtggac gaccgcgccg cggccgcgtt ccaacatctg cggcagcaac
      121 gcccggtcca gacgcaccgc cgtcatcagg ttgaggtcca gttccgcggc ccagtgatcg
      181 tcggtgagcg cggcgaagcc gccgccgggt gacgacgagc cgcccaggac gtggacgagg
      241 acatcgacgc cgccgaggcg ttcgatcgcg gctctcgcca gctgttcagc gccgtccggc
      301 ctcgtcaggt cgacttccac gacatgcgcg cccgccgggg cgttggcggc gctgcgcgcg
      361 gcggtgacga cgctggcgcc gccctgcagc agccgttcca ccgttgcccg gcccgcgccc
      421 ttggtgcctg cggtgaccac ggcgcgcacg ccttcgaagt cgcgttgctc gccggacatc
      481 agacgatctc cagagccgaa atggccccgc cgctcagctg aaagcggaaa gcgaacggaa
      541 ggggacttcc cgggaagtcc ccggcggttg tcccttcgac gatgacggcg ccgtcggtct
      601 cgcgccagga cgtcggcgtg aagattgccc gcgcggctat ggtcgccgac tggatccagg
      661 gcttgatctc ggcatggccg ttatgttcgc gcgcttcgtc cttcacgacg gcgtcggcgg
      721 cgaaggcggc aagcatgccg tccacgtcga ggcgggcatt ggcctccacg tagtcggcga
      781 cagggcgggg gagtttgatg gacaccgatg gtctccggcg taaggttgct ggccgccgga
      841 aactgtgggc atcctcccga aatgacaaga accgacgaaa aagtaaggta cgcaccagaa
      901 agtaagcgcg tggcctactc ggcggactcg gcgtctctga gcatccagaa tgttattcgg
      961 gtgctggaag ggcggtggaa gctggtgatc ctgtttcagc tgttcggtgg ccgggtgcag
     1021 cggttctcgg acctggagcg cgctatccct ggggtgtccc agaagatgct gatccagcag
     1081 ctacgacaac ttgaggccga cggggtcgtg acccggatcg tccacccgca ggtgcctccg
     1141 aaggtcgagt atcatctcac cgattggggg cagtcccttt gccccgccct ggacgccctg
     1201 ttgatctggg ccgagcaggc gcccgctgaa cagcgttcaa ggttgatccc tgaacctctg
     1261 tagatggtcg tcgcctccaa cggtcgtagc gacgcgatcg ttcgccacgt ctagtcggcg
     1321 aacgtcacgt ttctggaggg acggcaatct ctggtgctgg cgccaagcgg tcgtgttccg
     1381 cctcccaacg aatggcgcgg gcggggtgaa acttaccctg gctgtaaggg cttatgtcaa
     1441 agcgcagcga taggtaggca acgcctcaaa cggacgtttg cttacggcgc agcaagcctc
     1501 ttcgcgatgt ctgggaaatg gtcgggaccc aaagcgttga attgctttgg gtcccgatgg
     1561 tgccacgaca gggactcgaa ccctggacct cagccttacc aaggatgcgc tctaccactg
     1621 agctaccgcg gcgaagagga gggggcgtat agcgagccaa atcgcggatg caaagcccaa
     1681 aagcgacttg acccacagac ttcgtcgccg cacccctcgc ccatgccgat cccagacgac
     1741 aaatccctcc gcgaagccag gctcgccgaa gcgctcagga ccaacctgcg caagcgaaaa
     1801 gccgcctcgc gcaggccgcg ccgcgcccct acagcgtcgt gcgtcgcctg gagggcgtcg
     1861 cgcatcgaga cgggacccga gtcgccctgg tgctggaaat ctccccgccc tatccggcgc
     1921 cggagtcgga cgaggtctgc tgcgccgtgc gactggtggg cgatggcggt cagttcgaca
     1981 ccgagcatgg caaggcggcg ttcggcgtcg acgggctgca ggcgatgaag cgggccctcg
     2041 acctggccca ggtggcgctc gatctggcca gcaccaccta cgacctgcgc tggcgggacg
     2101 gccaatccta cgacctttcc gcgcccattt gagccgcgcc ccttgcaagc cctcttgcaa
     2161 cggatagaac ccgcccctca catgtgaccc gcgcggcgct tacgtctccg tcgcgtgatc
     2221 gaggaatgtc atttggatcg catcgccatc atcggcggcg cgcggctgca cggggaaatc
     2281 ccggtcagcg gcgccaagaa ctcggccatc aagctgatgg ccgccagcct gctcaccgac
     2341 gagccgctgc gcctgaccaa catgccgcgc ctggccgaca cccggttcct gggcaagctg
     2401 ctgggccggc tgggcgtcag cgtcgtcgaa agcgacggcc ccgacggtca gcagacggtt
     2461 ctgcacgcgc cggagatcac cagcggtttc gcgccctatg acctggtgcg ccagatgcgc
     2521 gcctccttca acgtgctggg cccgctggtc gcccgcaccg gccaggccaa ggtcagcctg
     2581 cccggcggct gcaccatcgg cgcgcggcct gtggacctgc acctgcaggc cctggaggcc
     2641 ctgggcgcca
//
LOCUS       DAOMEO010000374         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4162_length_2648_cov_1.809873, whole genome shotgun sequence.
ACCESSION   DAOMEO010000374 DAOMEO010000000
VERSION     DAOMEO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_2648_cov_1.809873"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLH31_09190"
     CDS             complement(<1..258)
                     /locus_tag="PLH31_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89418.1"
                     /translation="MTALSPVDAALEGLRTMRRHPVVVLAWAGFSLVMLPLLGLLAKI
                     VLSEQDRMNLALRQSSADPREILDFVSRLGGVMVLLIMLALV"
     gene            complement(259..1059)
                     /locus_tag="PLH31_09195"
     CDS             complement(259..1059)
                     /locus_tag="PLH31_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89419.1"
                     /translation="MTDGSVAIGATVRAGVSGLGPSVRACWGALLAAVVLSVLTRALP
                     SGLSGLALLVEIVAIALVSGALYRRAFGKPAGLAGLRWGLDEWRLLGVQALIAVFLFV
                     VATVLILVVGAIALGVARANAPGFDATSSQAWRDALAVSGPGAIVAGAAPLLGLAVLI
                     WLAMRLSLAGPATVDQSGVRVLSAYPLTRGVTLQVLAAGLLLAAPVAVLVAISSLLGR
                     WAGPGLDGVLAALGAILGYGYLAPAWAAALVHVYRRRHGPDASSSQEV"
     gene            complement(1204..2142)
                     /locus_tag="PLH31_09200"
     CDS             complement(1204..2142)
                     /locus_tag="PLH31_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89420.1"
                     /translation="MAKISVAGSAFAGFGVIARHPGAVLTWGVVMLVLSLAPVLALFA
                     ILGPEMLKTLTAMMPPKDGPIDQEAVRQLMQLQSGMMLVQIGGWLWSTFVKAIICSAV
                     FRAVLEPNQSRGAFLRLGAQELWLTLLFLVVSVLAYIVAVIASIGVVLPALIVGLAGG
                     AQGQGAMAGVLTGFGLGLVAVGVLVWLGLRLSLAAPMTFADRQFRLFESWSVTRGSAW
                     RLLGVAVLCLLMVLAVELVVAGVVLGAIFAAGGPPAWLSDPVAVQAYFTQPPLELLGR
                     LWPWLAGVGVVWTLIGSCLIAVFCAPWAVAYRDLTR"
     gene            2240..>2648
                     /locus_tag="PLH31_09205"
     CDS             2240..>2648
                     /locus_tag="PLH31_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR89421.1"
                     /translation="MSTSTPPLAPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRG
                     TDAHHTDGLAEMVCSNSFRSDDWQFNAVGLLHAEMRRMDSLILSAADQHQVPAGGALA
                     VDHDGFSAEVTRRLEAHPLVTIQREEVAGLPPKD"
BASE COUNT          487 a         1020 c          873 g          268 t
ORIGIN      
        1 caccagggcc agcataatca gcagcaccat caccccgccc agccgcgaga cgaagtcgag
       61 gatctcgcgc gggtcggccg acgactggcg cagggccagg ttcatgcggt cctgctcgct
      121 gagcacgatc tttgccagca ggcccaacag gggcagcatc accagcgaga acccggccca
      181 ggccagcacc accacgggat gccgacgcat ggtgcgcagg ccttcaaggg cggcgtcgac
      241 gggcgatagc gcggtcatct agacttcctg cgaggacgag gcgtccgggc cgtggcggcg
      301 gcgatagacg tgaacaagcg cggcggccca agcgggcgcc agatagccat agcccaggat
      361 cgcgcccagg gccgccagca ccccgtcgag gccggggccg gcccagcggc ccagcaggct
      421 ggagatcgcc acaagtacgg cgaccggcgc cgccagcagc aggccggcgg ccagcacctg
      481 cagcgtgacg ccgcgcgtca gcggataggc gctgagcacc cgcacgcccg actggtccac
      541 cgtggccggc ccggccagag acagccgcat ggccagccag atcagcaccg ccaggccgag
      601 cagcggcgcg gcgccggcga cgatggcccc cggacccgaa acggccagcg cgtcgcgcca
      661 cgcctgggac gaggtggcgt caaagcccgg cgcattggcc cgcgccacgc ccagggcgat
      721 ggccccgacc accaggatca gcacggtcgc cacgacaaac aggaagaccg cgatcagggc
      781 ctgcacgccc agcaagcgcc actcgtccag cccccagcgc agacccgcca ggccggcagg
      841 cttgccaaag gcgcggcgat agagggctcc ggacaccagg gcgatggcga cgatctcgac
      901 cagcagcgcc agaccgctca ggccggacgg cagggcccgg gtcaggacgc tcagcacgac
      961 agcggccagc aaagcgcccc agcaggcgcg gacggaggga cccagccccg agacccccgc
     1021 ccgaacggtc gctccgatcg cgaccgatcc atccgtcatc gcacgcccct cgtgaacccg
     1081 ctccgcgcga gtcgcgcggg ctggcctgtt tacgacgccc cgcgccgggt tgcgacgcgt
     1141 cgcggcggtc agcggacgaa ttttggatgg ccgtggcgcc ggcgccgcgt cgccctggag
     1201 cccctagcgg gtcaagtccc ggtaggcgac ggcccagggc gcgcagaaca ccgcgatcag
     1261 gcacgagccg atcagggtcc agaccacgcc cacccccgcc agccacggcc acagacggcc
     1321 aagcagttcc agcggcggct gggtgaaata ggcctggacc gccacgggat cgctcagcca
     1381 cgcgggcggc ccgccggccg caaagatcgc gcccagcacg acgccagcca caaccagctc
     1441 cacggccagc accatcagca ggcacaggac cgccacgccc agcaaccgcc aggccgaccc
     1501 gcgcgtcacc gaccaggatt caaacaggcg gaactggcgg tcggcaaagg tcatcggcgc
     1561 ggccagcgac aggcgcagcc ccagccagac cagcaccccc accgcgacca ggcccagccc
     1621 gaacccggtc agaacaccgg ccatcgcgcc ctggccctgc gccccgccgg ccaggcccac
     1681 gatcagggcc ggcagcacga cgccgatcga ggcgatgacg gcgacgatat aggccagcac
     1741 gctgaccacc aggaacagca gggtcagcca cagctcctgc gcgcccagcc gcaggaaggc
     1801 gccccgcgac tggttcggct ccagcacggc gcggaagacg gccgagcaga tgatcgcctt
     1861 gacgaaggtg ctccacagcc agccgccgat ctgcaccagc atcatgccgg actgcagctg
     1921 catcagctgg cgcacggcct cctggtcgat gggcccatcc ttgggcggca tcatcgccgt
     1981 cagggtcttc agcatctcgg gccccaggat cgcgaacagc gccagaaccg gcgccagcga
     2041 caggaccagc atcaccacgc cccaggtcag gaccgctccg ggatggcgcg cgatgacgcc
     2101 aaagcccgcg aacgccgaac ccgccaccga gatcttggcc atctgcttcc cccatgcgcg
     2161 ccggacgcga aacacgccgg atttctcgtg agcctacacc caggtttcgt tcggcgacag
     2221 ccgggggtat gagggcgcca tgagcacctc gacgccccct ctcgcccccg tccacgtcat
     2281 tggcggcggc ctcgccggat ccgaagccgc ctggcagatc gcccaggccg gcgtgcctgt
     2341 cgtgctgcac gagatgcggc cggttcgcgg caccgatgcg caccatacgg acggtctggc
     2401 cgagatggtc tgctccaact cgttccgctc cgacgactgg cagttcaacg ccgtgggctt
     2461 gttgcacgcc gagatgcgcc ggatggactc gctgatcctg tcggccgccg accagcatca
     2521 ggtgcccgcc ggcggcgccc tggccgtcga ccacgatggc ttctcggccg aggtcacccg
     2581 acgcctggag gcccaccctc tggtcacgat ccagcgcgag gaagtggccg gcctgccgcc
     2641 caaggact
//
LOCUS       DAOMEO010000375         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4165_length_2647_cov_2.561343, whole genome shotgun sequence.
ACCESSION   DAOMEO010000375 DAOMEO010000000
VERSION     DAOMEO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4165_length_2647_cov_2.561343"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="PLH31_09210"
     CDS             <1..117
                     /locus_tag="PLH31_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89422.1"
                     /translation="RYIAAPQKWAIPFLLADIGLLTSGGVGGLTKRPKRPAT"
     gene            complement(114..329)
                     /locus_tag="PLH31_09215"
     CDS             complement(114..329)
                     /locus_tag="PLH31_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89423.1"
                     /translation="MDLPFTVIALIVLLTLAVFCGWRGARPADILKGPRMVPWRPLMM
                     ACAVAIMLLLVHLLNLLGVQTGAQTRY"
     assembly_gap    460..523
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            643..>2647
                     /locus_tag="PLH31_09220"
     CDS             643..>2647
                     /locus_tag="PLH31_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Localization factor PodJS"
                     /protein_id="HQR89424.1"
                     /translation="MTLGEWLNRMIIEGEGPGADAPAADAFGGGAQDRSPKPYLHIAS
                     HGDTPPRIEIAEHPADEVGRVAIALDRLTDRIEAAEGRSAAAINGIDQSVRGALSRLA
                     LSEREQIAVAARFEGAVDEIKTEQTRAAERLRRMEAEAVGPRSAEALRALEGALGKVA
                     GHLYEGEARTREAIAALEARLDAQPAATSDPTAVVEEVVARLGQRLEAAETRTAEALR
                     ELGSSFSALDGRLQAVEGANPATGVQERLDQLAGDLTKRMEAARLEMATKLRDSADGR
                     FDRMEKKLGEMTAHVQAAEHRSAQAIERMGREVVGIAEVLNRRMQASEQRNTAAIEQV
                     GGEVARIASTVEHKLNRADSVQAQALEKLGAEIARITEKLAERIGSAERRNALAIDDV
                     GEQVTRVTDRLNQRHERSSQELIDRIRQSEERTARMLDEAREKIDSRLSDAQRKLAEA
                     PVQAAVAAPAPVVRAPAVSPFETSSYSFGGEPALDEDVFNAPPPQAARKPVFEVAAFP
                     AVDPEPPSFEDEDYEAADGFVGQQPSEAVESFAPASDFGTFSAPEPTDPSRPLSTREV
                     IEQARAAARAAAAADVKAKKDHKKDKARDKPARTSLFAGFGSKTAAKPKRRIGSSVTT
                     ALMVFGIAGAMGGGVGLLLLPNGGASQTPDRVAEAIAGRKADVE"
BASE COUNT          466 a          906 c          850 g          361 t
ORIGIN      
        1 cgctatattg ccgcgccgca gaagtgggcg atcccttttc tgctcgccga catcggtctg
       61 ctgacttcgg ggggcgtcgg cggcctgacc aagcggccaa aacggcccgc gacctaatag
      121 cgcgtctggg cgcccgtctg cacgcccagc aggttcagca ggtggaccag aagcagcatg
      181 atcgccacag cgcaggccat catcagcggg cgccagggaa ccatgcgcgg gccctttagg
      241 atatcggccg ggcgggcgcc gcgccatccg cagaacacgg cgagggtcag cagcacgatc
      301 agggcgatga cagtgaaggg caggtccatg agcgttccat gccctgcgac aatcggcctg
      361 gcaaatggtt aacctgcgtt aacccccttg ctttttaacc aaggtaaaca tggttaaagc
      421 tgtgtcccga ttcctccacc tgtgaatcgc aaacctactn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcgttg gtccccaagt
      541 acggggagaa cgattcgtat gacggccgca gcgccatgga gcgtaaaggg aattgatccc
      601 aaggcacgag aggtcgcaaa ggacctcgcg cgtcgctccg gcatgacgtt gggcgagtgg
      661 ctcaaccgca tgattatcga gggtgaaggc ccaggcgcgg atgcgccggc cgctgacgcc
      721 tttggcggcg gcgcccagga tcgttcgccc aaaccctatc tgcacatcgc ctcgcacggc
      781 gatacgccgc cccgcatcga gattgccgag caccccgccg acgaagtcgg tcgcgtagct
      841 attgcgctcg accgtctgac cgatcgcatc gaggctgccg aaggccgctc ggccgccgcc
      901 atcaatggca tcgaccagtc ggtgcgcggc gcgctgagcc gcctggcctt gtccgaacgc
      961 gaacagatcg ccgtcgccgc ccgtttcgag ggcgcagtcg acgagatcaa gaccgaacag
     1021 acccgcgccg ccgaacgcct tcgccgcatg gaggccgaag ccgtcgggcc acgttcggcc
     1081 gaagccctgc gcgcgctcga aggcgcgctc ggcaaggtcg ccggacatct ctacgaaggc
     1141 gaagcccgca cccgcgaagc gatcgccgcc ctggaagccc gccttgacgc gcagcccgcc
     1201 gcgaccagcg atccgaccgc cgtggtcgag gaagtcgtcg cacgtctggg ccagcgcctg
     1261 gaggcggccg aaacccgcac cgccgaggct ttgcgcgaac tgggctcatc cttctccgcc
     1321 ctggatggcc gtctgcaggc ggtcgagggc gccaatcccg cgacgggcgt tcaggaacgt
     1381 ctcgaccagc tggccggcga cctgaccaag cgcatggagg ccgcgcgtct cgaaatggcg
     1441 accaagctgc gcgacagcgc cgacggtcgc ttcgatcgca tggaaaagaa gctcggcgag
     1501 atgaccgccc acgttcaggc cgccgagcat cgctcggccc aggccatcga gcggatgggg
     1561 cgtgaagtcg tcggtatcgc cgaagtcctg aaccgtcgga tgcaggcgtc cgagcaacgc
     1621 aacaccgccg ccatcgagca ggtgggcggc gaggtcgcgc gtatcgcctc gacggtcgag
     1681 cacaagctga accgcgccga cagcgtccag gcccaggccc tggaaaaact gggcgcggaa
     1741 atcgcccgca tcaccgaaaa gctggccgag cggattggta gcgccgagcg tcgcaacgcc
     1801 ctggccatcg acgatgttgg cgagcaggtc acccgcgtca ccgaccgcct gaaccagcgc
     1861 catgagcgtt cgtcgcagga actgatcgac cgcatccgcc agagcgaaga gcgcaccgcg
     1921 cgcatgctcg acgaggctcg cgagaagatc gacagccgct tgagcgacgc ccagcgcaag
     1981 ctggccgagg cgccggtcca ggccgctgtc gccgcgccgg ctccggtcgt tcgcgccccg
     2041 gctgtctcac cgttcgagac cagcagctat tcctttggcg gcgagcctgc cctggacgag
     2101 gacgtcttca acgcccctcc gccccaggct gcccgcaagc ccgtgttcga ggtcgccgcc
     2161 ttcccggcgg ttgatcccga gcccccaagc tttgaggacg aagactatga ggcggcggac
     2221 ggctttgtgg gccagcagcc ctcggaggcg gtcgagagct tcgccccggc ctccgacttc
     2281 ggcaccttct cggccccgga accgaccgat ccctcgcgcc cgctgtcgac gcgcgaagtg
     2341 atcgagcagg ctcgcgccgc cgcccgcgcc gcggccgccg ctgacgtgaa ggccaagaaa
     2401 gaccacaaga aggacaaggc cagggacaag ccggcgcgca cctcactgtt cgcgggcttc
     2461 gggtccaaga ccgcggccaa gcccaagcgc cggatcggct cgtccgtgac cacggccctg
     2521 atggtgttcg gcatcgccgg cgccatgggc ggcggcgtgg gcctgctgct gctgcccaat
     2581 ggcggcgcca gccagacgcc tgaccgcgtt gctgaagcca tcgccggccg caaggccgat
     2641 gtcgagg
//
LOCUS       DAOMEO010000376         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4167_length_2645_cov_2.771815, whole genome shotgun sequence.
ACCESSION   DAOMEO010000376 DAOMEO010000000
VERSION     DAOMEO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_2645_cov_2.771815"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLH31_09225"
     CDS             <1..494
                     /locus_tag="PLH31_09225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQR89425.1"
                     /translation="ILTPDFLRKDRAENVVIDARPDVFNHNLETVPRLYLKIRPGARY
                     YNSLRLLERVKERDPSQFTKSGLMVGLGEAKEEVMQVMDDMRSAGVDFITIGQYLQPT
                     RKHAAIDRFVTPEEFKAYEAIARAKGFLMVSSSPLTRSSHHAGEDFAKLQAARRALDA
                     RMA"
     gene            497..952
                     /locus_tag="PLH31_09230"
     CDS             497..952
                     /locus_tag="PLH31_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQR89426.1"
                     /translation="MHRHVVTRVLPYSPEQLFALVGDVEAYPSFVPWITGMRTWNARQ
                     DGSISLVDAEAQVGFSFLKERFATRVRRDAEHLTVDVSLLYGPFKRLSNQWRFTPNSA
                     GTSVEFVIDFAFKSRMLDALLAANLDRAADKLIACFEARARQIHGASLD"
     gene            complement(939..1439)
                     /locus_tag="PLH31_09235"
     CDS             complement(939..1439)
                     /locus_tag="PLH31_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQR89427.1"
                     /translation="MFPLEIETLARLLVAEAREQSLRLVTAESCTGGLVAGAICSISG
                     ASDVFERGFVTYTNRAKSEMLKVPGDLIADHGAVSQPVARMMAEGALEASNGHVTVSI
                     IGVAGPGAGTPMKPVGTVHFAVARANRSVVHRHEVFDGQTREAVQLAAVRTALEMLRE
                     AVASRG"
     gene            complement(1475..2626)
                     /locus_tag="PLH31_09240"
     CDS             complement(1475..2626)
                     /locus_tag="PLH31_09240"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HQR89428.1"
                     /translation="MTFSAVIVAAGSGTRAGPGQAKQWRSLGGKPVLRWSVESFLVAG
                     AAEIVVVTTEDGALVLPEVLKGLRGWKGARGGATRALSVQAGLGALSDRPADEPVLVH
                     DAARPFLTRTTVDSLLSALVGKDGAFPALAVADTLKRAAPGGAVVTTSRDHLWRAQTP
                     QAFGRATLVSAYEAWDGDTEPTDDAQVVEAAGGSVTIAPGDPLLMKLTYPEDFAMAER
                     LAGAARITRIGQGFDAHRWGPGGSVWLCGVEIAHDETLIGHSDADAGLHALTDALLGA
                     IGEGDIGDHFPPTDPQWKGAASDKFLIHAAELVRAKGGSIINVDVTLICERPKIKPHR
                     AAMRERLAEILSLPIDRVSVKATTTEQMGFTGRGEGLAAQAVVAVETPA"
BASE COUNT          444 a          872 c          859 g          470 t
ORIGIN      
        1 agatcctgac gccggacttc ctgcgcaagg atcgcgccga gaacgtggtg atcgatgccc
       61 gtcccgatgt cttcaaccac aatctggaga ctgtgccgcg gctctatctg aagatccgcc
      121 ccggcgcccg ttactacaac tccctgcgcc tgctggagcg ggtgaaggaa cgcgacccga
      181 gccagttcac caagtcgggt ctgatggtcg gtctgggcga ggccaaggaa gaggtcatgc
      241 aggtcatgga cgacatgcgc tcggccggcg tcgatttcat caccatcggc caatatctgc
      301 agcccacccg caagcacgcc gccatcgacc gctttgtgac cccagaagag ttcaaggcct
      361 atgaagctat tgcgcgggcc aagggcttcc tgatggtctc atccagtccg ctgactcgct
      421 cgtcccacca cgccggcgag gatttcgcca agctgcaggc agcgcggcgc gccctcgacg
      481 ccaggatggc ctaggcttgc atcgccacgt cgtcacgcgg gtcctgccgt actcgcccga
      541 gcagttgttc gcactggtcg gcgatgttga ggcctatccc agcttcgtgc cgtggatcac
      601 cggcatgcgg acctggaatg cccgccagga tggatccatc agccttgtgg acgccgaagc
      661 ccaggtcgga ttttcctttc tgaaggagag gttcgctacg cgtgtgcggc gcgacgccga
      721 gcatctgacc gtcgatgtca gcctgctcta tggccccttc aagcggcttt ccaaccagtg
      781 gcggttcacg cccaacagcg ccggaaccag cgtcgagttc gtgattgatt tcgcgttcaa
      841 atcgcggatg ctcgacgcct tgctggcggc taatctcgac cgggcggctg acaagctgat
      901 cgcctgtttc gaggctcgcg cccggcagat ccacggtgct agcctcgact agccaccgcc
      961 tcgcgcagca tttcgagcgc tgtgcgaacc gcggccaatt gcaccgcctc gcgggtctgg
     1021 ccgtcgaaca cctcgtgccg atgcaccact gaacggttgg cgcgggccac agcgaaatgg
     1081 acagtgccga cgggtttcat tggggtgccg gcgcccgggc cggcgactcc tattatcgag
     1141 acggtcacgt gcccgttgct ggcttccagc gccccttcgg ccatcatcct ggccacgggt
     1201 tgtgaaaccg cgccatggtc ggcgatcaga tcgcccggaa ctttcagcat ctcggacttc
     1261 gcccgatttg tgtaggtcac aaagccgcgc tcgaagacat ctgacgcgcc cgatatcgaa
     1321 catatcgcgc ccgctaccag accgccggtg cagctctcgg cggtcactag ccgcagggac
     1381 tgttcacgcg cctcggcaac caggaggcgg gccagggtct cgatctctag aggaaacatg
     1441 gggcgctccg gattcgttgt tcagcctata ggcctcaggc cggagtctcg accgctacga
     1501 cggcttgagc ggcgaggccc tcgccgcggc cggtgaagcc catttgttcg gtcgtcgtgg
     1561 ccttgacgct cacccggtcg atcggcagcg acaggatttc ggcgaggcgc tctcgcatcg
     1621 ctgcccgatg gggtttgatc ttggggcgct cgcagatcag ggtcacgtcg acattgatga
     1681 tcgagccgcc cttggcccga accagttcgg cggcgtggat caggaacttg tcggaggccg
     1741 cgcctttcca ttgcggatcg gtcggcggga aatggtctcc gatgtcgcct tcgccgatgg
     1801 cgccgagcaa ggcgtccgtc agggcgtgca ggcctgcgtc agcgtcggaa tgaccgatca
     1861 gggtctcgtc atgggcgatc tcgactccgc agagccagac cgagccgcct gggccccagc
     1921 ggtgggcgtc gaagccctga ccgatccggg tgatgcgggc ggctcccgcc agtcgttcag
     1981 ccatggcgaa gtcctctgga taggtcagtt tcatcagcaa cggatcgccg ggcgcgatgg
     2041 tcacggagcc gccggcggcc tcgaccacct gggcgtcatc ggtgggctcg gtgtcaccat
     2101 cccaggcctc gtaggccgag accagggtgg cgcgtccaaa tgcttgcggc gtctgggcgc
     2161 gccacaggtg gtcgcgcgaa gtggtgacca cggcgccgcc gggcgcggcg cgcttcaagg
     2221 tatcggcgac ggccagggcg ggaaacgccc catccttccc gaccagggcg cttaacaggc
     2281 tgtcgacggt ggtgcgtgtc agaaaggggc gagcagcgtc gtggacaagg acaggctcat
     2341 cggctggccg atctgaaagc gcgcccagac cggcctggac ggaaagtgcg cgagtcgcgc
     2401 caccccgtgc gcctttccag ccccgcaacc ccttcagcac ctcgggcagg accagagcgc
     2461 cgtcctccgt ggtgacgaca acgatctcgg cggccccggc gaccaggaag ctctcgaccg
     2521 accagcgcaa aacgggttta ccccccaggg atcgccattg cttggcttgt ccagggccgg
     2581 cccgggttcc ggagccagcg gcgacgatca cggcggaaaa ggtcatggcc ggtgtttagt
     2641 tcgac
//
LOCUS       DAOMEO010000377         2641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4172_length_2641_cov_2.758314, whole genome shotgun sequence.
ACCESSION   DAOMEO010000377 DAOMEO010000000
VERSION     DAOMEO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4172_length_2641_cov_2.758314"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLH31_09245"
     CDS             <1..319
                     /locus_tag="PLH31_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89429.1"
                     /translation="EIDQLARDTLGLTADEAAVIPDPEGWDGIDIRSMREQADQFELE
                     GWDVTDDKRRPLRILGHFSHPLWLALRGVAGKLPFFPEPEETEHTTSSLAAEAKAFQR
                     DKR"
     gene            complement(316..498)
                     /locus_tag="PLH31_09250"
     CDS             complement(316..498)
                     /locus_tag="PLH31_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HQR89430.1"
                     /translation="MGIPKGKRDFRDEDRLDPRERHQVEYWMKRWGVTKDQLIAAHRS
                     AGNSIKDIATELGKKR"
     gene            634..876
                     /locus_tag="PLH31_09255"
     CDS             634..876
                     /locus_tag="PLH31_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89431.1"
                     /translation="MAADELSRAMTLTWRDLSKVIPWGDTFEGISPGGRDVEVERNYL
                     WATDPGGDILCEVAVYGGASRYDQGARARGVISRKG"
     gene            961..1776
                     /locus_tag="PLH31_09260"
     CDS             961..1776
                     /locus_tag="PLH31_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89432.1"
                     /translation="MYQSEFPVDQPVPIFDPTLLMGLILAAVVIAALAFAAGRLLGRK
                     DNSEGYRGVAKTIHEAIKAKCVAATSAPSGELVSKTQELIEEVDRRIGAIIKFGGPCA
                     KAMKGLTEALKGEPAEEKDKGKDAHGHGHAQGGGHGPGPGDHGGHGVGGHGGHGGHDP
                     SCQCDRCVVAVVEAAVPNVTILGPRIGGQTVVISNPASPAKPADDHHAQHDAKVLDHK
                     DFSKQVRMAVVEFSDFWSRGDCLLELERCQAMLTSTAPFPDVKALKLKAQSHH"
     gene            2013..2519
                     /locus_tag="PLH31_09265"
     CDS             2013..2519
                     /locus_tag="PLH31_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQR89433.1"
                     /translation="MIATVSPLTTATPEPPEEALSLADYVPYRLVTAAAAVSRLIARA
                     YEDKFGLTIPQWRLMSVLAEGGLTPHAAVARTAMDKVRVSRAAQDLVERRLVNRTTVR
                     ADRRSHRLELTAAGRRLFAEIAPMALAYEAALLSGLAPSEVVTLKRLLGRLEVTATQL
                     AGGETMVP"
BASE COUNT          478 a          809 c          885 g          469 t
ORIGIN      
        1 agagatcgat cagctggcgc gtgacaccct cggtctcacg gctgacgagg ccgccgtcat
       61 tcctgatccg gaaggctggg acggaatcga tatcagatcg atgcgcgagc aggccgatca
      121 gttcgagctg gaaggctggg acgtgaccga cgacaagcgt cggccgctgc gcattctggg
      181 ccatttcagc catcccctgt ggctggccct gcgcggcgta gccggcaagc tgccgttttt
      241 tccagaaccg gaggagacgg aacacacgac atcatccctg gctgcggaag ccaaggcgtt
      301 ccagcgcgac aagcgttagc gcttcttccc cagttcggtg gcgatatcct tgatggaatt
      361 gccggctgat cggtgggccg cgatcagctg gtccttggtc acgccccagc gcttcatcca
      421 gtattcgacc tggtggcgct cgcgggggtc gagccggtct tcgtcgcgaa agtcgcgctt
      481 gcccttggga atgcccatga tcatctcctg cgtccgtccg cgacctatcc tgcgctctgg
      541 ccgttgtcga ggcgacgccc aaagctcacc tttgttccaa tgatccgaaa ccatgaagcg
      601 cccgcaccga tgagcgatcc cgatcttgaa gccatggcgg ccgatgaact gtcgcgcgcc
      661 atgaccctga cctggcgcga cctgtccaag gtgattccct ggggcgacac gttcgagggc
      721 atctcgcccg gcggtcgcga tgtggaggtg gagcgcaact atctatgggc gacggacccg
      781 gggggcgaca tcctgtgcga ggtggccgtc tatggcggcg cgtcacgata tgatcagggc
      841 gcgcgagccc gaggcgtgat ttcccgcaaa gggtgacaac ctaagccgtg caagtggtct
      901 ctggttgcgt taaccatggg ccgcgccatc atggccaagt cacagcgcgg ggaggggtcg
      961 gtgtaccagt cagagtttcc ggtcgatcag cccgtgccga tttttgatcc gaccctgctg
     1021 atgggtctga tcctggcggc ggttgtgatc gccgccctgg cctttgccgc tggccgcctc
     1081 ttgggccgca aggataatag cgaaggttat cgcggcgtcg ccaagaccat tcacgaggcc
     1141 atcaaggcaa aatgcgtcgc cgccaccagc gcgccgtcgg gcgaactggt gtcgaagacc
     1201 caggagctga tcgaggaagt tgaccgacgc attggcgcga tcatcaagtt tggcggccct
     1261 tgcgccaagg cgatgaaagg gctgaccgag gccctgaagg gcgagcccgc cgaagaaaag
     1321 gacaagggca aggatgccca tggtcatggt catgcgcagg gcggtggtca cggtcccggt
     1381 ccgggcgacc atggtggtca cggcgtcggc ggacacggtg gccatggcgg ccacgatccc
     1441 agctgccagt gcgaccgttg cgtcgtggcg gtggtcgagg cggccgtgcc gaacgtgacg
     1501 attctcgggc cccggatcgg cggccagacc gtcgtcatct ccaatcccgc tagtcccgcc
     1561 aagccagccg acgatcacca cgcacagcac gacgccaagg tcctggatca caaggacttt
     1621 tcgaaacagg tgcgtatggc ggtggtggag ttctctgatt tctggagccg gggcgattgt
     1681 cttctggagc ttgagcgctg ccaggccatg ctgacgagca cagcgccttt tccggacgtg
     1741 aaggcgttaa aactgaaagc acaatcgcat cactaagaag gtcccggcgc ccacggggcg
     1801 tgcgcgggat ttaaagaatg cacaaccacc acgcgaatgc aggcgtttct gctgtggttg
     1861 cggctggacg attgaggcga cagggcgcta ggcgccacat cgactggcgc gaagcgagcc
     1921 gatccatggt cggcctacgt aacgtcgctt aggatcggcg aaggttgtct tccaaatggc
     1981 gaccgcgccc gggaggacga aaggaacggg tcatgatcgc aacggtctcc cctttgacga
     2041 cggcgacgcc cgaaccgcca gaagaggcgc tcagcctggc cgactatgtg ccgtatcgac
     2101 tggtcacggc cgcagcagcg gtcagtcggc tgatcgcgcg cgcctatgag gacaagttcg
     2161 gattgaccat cccgcaatgg cggctgatgt cggtgctggc cgagggcggg ctgacgcccc
     2221 acgccgccgt ggcccgcacg gcgatggaca aggtccgcgt cagtcgcgct gcccaggatc
     2281 tggtcgagcg acgattggtc aaccgcacga ctgttcgggc agaccgtcgc tcccatcggc
     2341 tggaactgac ggcagctggt cgacggctgt tcgccgagat cgcgccaatg gcccttgcct
     2401 atgaggcggc gttgctgtcc ggcctcgcgc ctagcgaagt ggtcacgctg aagcgcctgt
     2461 tgggacgcct cgaagtcacc gcgacgcagt tggcgggcgg cgagaccatg gtcccctgaa
     2521 ggctccgttg cctgtagggc gcgcagatcg cgcccgccgc tctctcggta ctggaattca
     2581 aatacgacgc ctgctagaga gacggccctt cgcgcgaggc gaagcacgcg ggcgtggtga
     2641 a
//
LOCUS       DAOMEO010000378         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4181_length_2637_cov_2.846243, whole genome shotgun sequence.
ACCESSION   DAOMEO010000378 DAOMEO010000000
VERSION     DAOMEO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_2637_cov_2.846243"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLH31_09270"
     CDS             complement(<1..567)
                     /locus_tag="PLH31_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR89434.1"
                     /translation="MSEVFQVERWRPGPLLPPRDARDGSLVFVVAVLCFLACLTALAA
                     LAANRAAEGWTSQLTGSATVVVRARPNETPDSAAARAAEALAGVKGVVEARALTKDKA
                     EELLVPWIGREALVDDLPTPRLVTLDLDPKLPATATALDQALKAAGVDAVIDDHSRWI
                     ADIERSAGLARWAALGVFALIAAAAASVI"
     gene            complement(564..1268)
                     /gene="ftsE"
                     /locus_tag="PLH31_09275"
     CDS             complement(564..1268)
                     /gene="ftsE"
                     /locus_tag="PLH31_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQR89435.1"
                     /translation="MRIEADQGDDALPVVRFEGVSMRYGRAPETLRDIGFSLAPGSFH
                     FLTGASGAGKSSLLKLVYLAHRASRGRVELFGRDVSLLPPGDLPFLRRRIGVVFQEFR
                     LLEHLSVFDNAALPLRILQRKPATYRDDVAELLTWVGLGERMHALPATLSGGEKQRLA
                     IARAVVDRPDVLLADEPTGNVDPAMSLRLLRLFVELNRLGTTVLIATHDEELVARAGR
                     PTLHLEHGRLVDGGDA"
     gene            1352..2305
                     /locus_tag="PLH31_09280"
     CDS             1352..2305
                     /locus_tag="PLH31_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQR89436.1"
                     /translation="MILTCPECASRYFVDDSKVGSAGRVVRCASCGNRWTARNEESTD
                     LFDEPSAATLAGDAAMAPMDAAAAATAGAASEPEPEPPVSALPGEELPKVFRARADAE
                     RRLREATTTGVVWAGMAAAMASMIVGALIFRIDVVRLWPTTAGAYASVGLPVNTVGLV
                     IENIKAEPSLEDGHAAVTITGIMRNITDQAVIAPPLRVELRNAKDKRVAGRIAAAADP
                     KIPPSEIRHFAITILDPPRTAKDLVIDFAVEPGAAKAVKAALKTPAKPAADPELSLRG
                     AQDAATGDAAGGEAAGHEPSGHETGAEAIPSSDSHEPAHHD"
     gene            2244..>2637
                     /locus_tag="PLH31_09285"
     CDS             2244..>2637
                     /locus_tag="PLH31_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQR89437.1"
                     /translation="MRPERKPSLPPTLTNPRIMIDTPKPEVLISEAEIAQRVQALANA
                     IAPRIDDDTVCICLLTGGLWFAADLTRALWKAGRNVRFDALWLASYHDERKSTGRCEV
                     RADLQRPLVGRRALVVDDVFDTGLSLSEA"
BASE COUNT          439 a          948 c          899 g          351 t
ORIGIN      
        1 gatcaccgag gccgccgccg ccgcaatcag ggcgaagacc cccagcgccg cccagcgggc
       61 cagacccgcg ctgcgctcga tgtcggcgat ccagcggctg tggtcgtcga tgacggcgtc
      121 gaccccggcc gccttcagcg cctgatccag agccgtggct gtcgccggaa gcttggggtc
      181 gaggtccaga gtcaccaggc gcggcgtcgg caggtcatcg accagcgcct cccggccgat
      241 ccaggggacc agcagttcct cggccttgtc cttggtcagg gcgcgcgcct cgaccacgcc
      301 cttgaccccg gccagggcct cggcggcgcg ggcggcggcg gagtcggggg tctcattggg
      361 ccgggcgcgg accaccacgg tggccgatcc ggtcagctga ctggtccagc cctcggcggc
      421 gcggttggcg gccagggcgg ccagcgccgt caggcaggcc aggaaacaca gcaccgccac
      481 cacgaagacc agcgagccgt cccgcgcgtc gcgaggcggc agcagcgggc ccggccgcca
      541 gcgctcgacc tggaacacct cgctcatgcg tcgcctccgt caaccaggcg gccatgctca
      601 agatgcaggg tgggccggcc ggcgcgggcg accagctcct cgtcatgggt ggcgatcagc
      661 accgtggtgc ccaggcgatt gagctcgacg aatagccgca gcagccgcag cgacatggcc
      721 ggatcgacat tgccggtcgg ctcgtcggcc agcagcacat cgggacggtc gaccacggcg
      781 cgggcgatgg ccaggcgctg cttctcaccg cccgacaggg tggcgggcag ggcgtgcatg
      841 cgttcaccaa gtccgaccca ggtcagaagc tcggcgacgt cgtcgcgata ggtcgccggc
      901 ttgcgctgca gaatgcgcag aggaagggcg gcgttgtcga acaccgacag gtgctccaag
      961 agccggaatt cctggaaaac caccccgatc cggcggcgca ggaagggcag gtcgccgggc
     1021 ggcaaaaggc tgacatcgcg gccgaaaagc tccacgcgac cccgtgacgc acggtgggcc
     1081 agatagacca gcttgagcag ggaactcttg cccgcgcccg aggcgccggt gaggaagtgg
     1141 aaagagcccg gagccagcga aaacccaata tcccttaggg tttccggcgc gcggccgtag
     1201 cgcatcgaca cgccttcgaa gcgaaccacc gggagggcgt catcgccctg atctgcctcg
     1261 atccgcaagg aggtcccgcc gggctttctg gacatcaggg ccaaaatcgg cgaccccatt
     1321 tgttaacgcg cggggcgtac gattcgaggc catgatacta acctgtccgg agtgcgccag
     1381 ccgctatttc gtcgacgact ccaaggtcgg gtcggcgggt cgcgtcgtgc gctgcgcttc
     1441 gtgcggcaac cgctggacgg cccgtaacga ggagtccacg gacctcttcg acgaaccctc
     1501 cgccgccacc ctggccggcg acgccgcgat ggcgccgatg gacgcggccg ccgccgcaac
     1561 ggctggcgcc gcttccgagc ctgaacccga accgcctgtc agcgccctgc cgggcgagga
     1621 actgcccaag gtgttccggg cccgcgccga cgccgagcgc cggctgcgcg aggcgacgac
     1681 cacgggtgtg gtctgggccg gcatggccgc cgccatggcg tcgatgatcg tcggggccct
     1741 gatcttccgt atcgacgtgg tgcgcctgtg gccgaccacg gccggggcct atgccagcgt
     1801 cggcctgccg gtaaatacgg tcggcctggt gatcgagaac atcaaggccg agccctcgct
     1861 ggaagacggc cacgccgccg tgaccatcac cgggatcatg cgcaacatca ccgaccaggc
     1921 cgtgatcgcc ccgccgctgc gggtcgaact gcgcaacgcc aaggacaagc gcgtggccgg
     1981 ccgtatcgcc gccgccgctg atcccaagat cccgcccagc gagatccgcc acttcgccat
     2041 caccattctg gacccgccgc gcacggccaa ggatctggtc attgacttcg cggtcgagcc
     2101 cggcgcggcc aaggcggtaa aagccgccct gaagacgccc gccaagcccg cggccgaccc
     2161 ggaactctcc ctgcgcggcg ctcaggacgc tgcgaccgga gacgcggctg gcggagaagc
     2221 ggctggacat gaaccctcgg gacatgagac cggagcggaa gccatccctt cctccgactc
     2281 tcacgaaccc gcgcatcatg attgacaccc caaagcctga agtcctgatt tcggaggccg
     2341 agatcgcgca gcgcgttcag gccctcgcca acgccatcgc gccacgcatc gacgacgaca
     2401 cggtctgcat ctgcctgctg accggcggtc tgtggttcgc cgccgacctg acccgcgcgc
     2461 tgtggaaggc gggccgcaat gtccgcttcg acgccctgtg gctggcctcc taccatgacg
     2521 agcgcaagag cacgggccgc tgcgaggtcc gcgccgacct gcaacgcccg ctggtcggcc
     2581 gccgcgccct ggtggtggac gatgtgttcg acaccggcct gtcgctcagc gaagcct
//
LOCUS       DAOMEO010000379         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4183_length_2637_cov_2.555383, whole genome shotgun sequence.
ACCESSION   DAOMEO010000379 DAOMEO010000000
VERSION     DAOMEO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4183_length_2637_cov_2.555383"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1204)
                     /locus_tag="PLH31_09290"
     CDS             complement(107..1204)
                     /locus_tag="PLH31_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQR89438.1"
                     /translation="MTQTPAPTGKIPVTVLTGYLGAGKTTLLNRILTEAHGKRYAVIV
                     NEFGEVGIDNDLVVGADEEVFEMNNGCVCCTVRGDLIRVLSGLMKRKGGFDAIIVETT
                     GLADPGPVAQTFFVDEDVKARTQLDSVTAVVDAKHIMLRLGDSREAVEQIAFADQIVL
                     NKTDLVSEDDLRHVEARIRRINPLAPIHRAQRSNVPLDAILGKHSFDLERITELEPQF
                     LNPAHGEAGHVHDEHCGHDHGHDHGHVHDEHCGHDHHGHDHGHASAIHDDGVKGISLT
                     LDKPVDGQKITVWLNDLLARRGPDILRAKGIIDVQGEERRLVFQAVHMILEGDFQRPW
                     TDKDQRYSRMVFIGRDLDEAELKAGFEATAA"
     gene            complement(1261..1905)
                     /locus_tag="PLH31_09295"
     CDS             complement(1261..1905)
                     /locus_tag="PLH31_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89439.1"
                     /translation="MNSTFFASIAATGFVVAFLHAALPTHWLPFVLVGRAQKWSTRRT
                     LGVTLLAGLGHVGLTIVLGLALVLAGMALQPSLGEVFHWIVGGLMAAVGVFYVLRAYF
                     LKEGHNHAMPEASRRTYGSDRAAIAALVTLLTLSPCEAFLPYYLAGMEHGWQAFLLLS
                     LVLLTATSAGMLIFTGLSLAGFKRLGLQWVERYEETILGVALILVGVAVAFFKT"
     gene            2104..2616
                     /gene="rplU"
                     /locus_tag="PLH31_09300"
     CDS             2104..2616
                     /gene="rplU"
                     /locus_tag="PLH31_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQR89440.1"
                     /translation="MYAVIKTGGKQYRVQAGDLLVVEKLDGEPGSEVAFGEVLMLGEG
                     EAVTVGAPTVDGAVVSGTLIETRKGEKVKIFKKIRRQGYRRTRGHRQTESVVRVTSVA
                     GAGKEIKWDGVIDLTPKVILNARARGLGDAAVPFTIPVAKTAAPVAEAAPKKKAAPKK
                     AAAAPEDGEA"
BASE COUNT          478 a          823 c          881 g          455 t
ORIGIN      
        1 ggtggtgtcg agaatcttcg tcatcttccc cactcctcga agccggcgtt ccaatttggc
       61 tcggaacgcc ggtgttctgg agtgaagatc gcaaggcttg ggccagttag gcggcggtcg
      121 cctcgaagcc ggctttcagc tcggcctcgt ccagatcgcg gccgatgaag accatgcggc
      181 tatagcgttg gtccttgtcg gtccaggggc gctggaagtc gccttccagg atcatgtgga
      241 cggcctggaa caccagacgg cgctcctcgc cctgcacatc gatgatgccc ttggcgcgca
      301 ggatgtcggg gccgcggcgg gccagcaggt cgttcagcca gacggtgatc ttctggccgt
      361 ccacgggctt gtccagggtc agggagatcc ccttgacgcc gtcatcgtgg atggcgctgg
      421 catggccatg gtcgtggccg tgatgatcat ggccgcaatg ctcgtcatgc acatggccgt
      481 gatcatgccc atggtcgtga ccgcagtgct catcatggac atgtcccgcc tcgccatgcg
      541 ccggattgag gaactgcggc tccagctcgg tgatccgctc caggtcgaag ctgtgcttgc
      601 ccaggatggc gtccagcggc acgttcgagc gctgggcgcg gtggatcggg gccagcgggt
      661 tgatgcggcg gatccgggcc tcgacgtggc gcaggtcgtc ttccgaaacc aggtcggtct
      721 tgttcagcac gatctggtcg gcaaaggcga tctgctcgac ggcttcgcgg ctgtcgccca
      781 gccgcagcat gatatgcttg gcgtcgacga cggcggttac cgaatcgagc tgagtgcggg
      841 ccttgacgtc ctcgtcgaca aagaaggtct gggccaccgg gccagggtca gccagacccg
      901 tggtctcgac gatgatggcg tcgaagccgc ccttccgctt catcaggccc gacagcaccc
      961 ggatcagatc gccgcgcacc gtgcagcaga cgcagccgtt gttcatttca aagacttctt
     1021 cgtcggcccc gaccaccagg tcgttgtcga tgccgacctc gccgaactcg ttgacgatca
     1081 cggcgtagcg cttgccgtgc gcctctgtga ggatgcgatt gagaagcgtg gtcttgccgg
     1141 cgccgagata gccggtcagc acggtgacgg ggattttgcc ggtgggagcg ggggtctggg
     1201 tgatggacgc tatttagggc gtctggcggc gcggtgaaag caccccagag gctttgggcg
     1261 tcaggtcttg aagaaggcga cggccacgcc gaccagaatc agggctacgc ccaggatggt
     1321 ttcctcgtag cgctccaccc actgcagacc caggcgcttg aagccggcca ggctcaggcc
     1381 cgtaaagatc agcatgcccg ccgaggtggc cgtcagcaga accaggctca gaagcaggaa
     1441 ggcctgccag ccgtgctcca tgccggccag ataatagggc aggaaggcct cgcagggcga
     1501 aagggtcagc agggtgacca gggcggcgat ggcggccctg tcagagccgt aggtgcgccg
     1561 gctggcctcg ggcatggcgt ggttatgccc ctccttcaag aaataggccc gcagcacata
     1621 gaagacgcca accgccgcca tcagcccgcc gacaatccag tgaaagacct cgcccagact
     1681 gggctgcagg gccatgcccg ccagcaccag ggccaggccg agcacgatgg tcaggcccac
     1741 atggccaagc ccggccagca gggtcacgcc cagggtgcgg cgcgtcgacc acttctgggc
     1801 gcggccgacc agcacgaacg gcagccaatg ggttggcagg gccgcatgca gaaaagccac
     1861 gacgaaaccg gtggcggcga tagaggcgaa gaaggtcgaa ttcacgctgg aggctatata
     1921 ctgttacatt gtaacgttcc agcctgaatc cgtcctgtgg agcgtcagcg tttcatggtc
     1981 aggaatctgt cgcagaccgt tgactcaagg tcggtcggct gtataagtcc gccccctcgc
     2041 ccgccgggtt tccttgcggg cgttccctat tttgcgaacg cacgtttctt aaggcgctaa
     2101 cctatgtacg cggtgatcaa aaccggcggc aagcagtacc gggtccaagc cggcgatctg
     2161 ctggttgtcg aaaagctcga cggtgaaccg ggctcggaag tcgctttcgg cgaagtcctg
     2221 atgcttggcg aaggcgaggc cgtgacggtc ggcgccccta cggtggacgg cgctgtcgtc
     2281 tccggaaccc tgatcgaaac ccgcaagggt gagaaggtga agatcttcaa gaagatccgc
     2341 cgtcagggct atcgccgcac ccgcggtcac cgccagaccg agtcggtcgt gcgcgtgacc
     2401 tcggtcgccg gcgccggcaa ggaaatcaag tgggacggcg tcatcgacct gacccccaag
     2461 gttatcctga acgcccgcgc tcgcggcctc ggcgacgccg ccgtgccctt cacgatcccg
     2521 gtggccaaga cggccgctcc ggtcgctgaa gccgccccca agaagaaggc cgcgccgaag
     2581 aaggccgcag ccgccccgga agacggcgaa gcctaagcgg ctgcgcctct agagatt
//
LOCUS       DAOMEO010000380         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4188_length_2636_cov_2.512205, whole genome shotgun sequence.
ACCESSION   DAOMEO010000380 DAOMEO010000000
VERSION     DAOMEO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_2636_cov_2.512205"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLH31_09305"
     CDS             complement(<1..211)
                     /locus_tag="PLH31_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQR89441.1"
                     /translation="MSFWPKSRKARRRLTILLAIAPVLALAVGLALYGMRDAISLFYT
                     PAQAQEAKVPAGRKVQLGGLVQAGSV"
     gene            complement(208..>1187)
                     /gene="ccmI"
                     /locus_tag="PLH31_09310"
     CDS             complement(208..>1187)
                     /gene="ccmI"
                     /locus_tag="PLH31_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HQR89442.1"
                     /translation="LAEGGLLDEAEHRAARAEAGRRLLAAADTAETWTRDGAGPRRLA
                     VAVAAVAPLLAVAAYVFIGSPGFSDQPFARRVAEWRATDPAQLDPPRIAAVLETLAAQ
                     RPNDAEPLKHLALARSASGDLAGAEQALRRAVRLAPGRADLWTGLGETFVAQANGQVT
                     GDAQVAFREALKIDPTSLSARYHLGRGKIGDGDLAGGLADWRALTADLPAGDPRRGAM
                     NREIAQVEAAGGIPAPEAAPEQSAQVQGMIAGMVDGLAARLASSPDDPDGWVRLVRAY
                     AVLGEVAKRDAAMASATRRYADQPKVLAALRQAAQTPPQQQTAPGGDRQ"
     assembly_gap    1188..1197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1198..1342)
                     /locus_tag="PLH31_09315"
     CDS             complement(<1198..1342)
                     /locus_tag="PLH31_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HQR89443.1"
                     /translation="MIAFWISAAGLSAAAGALVLRSAARAGATQGVSPDLDAHKRQLG
                     EIDR"
     gene            complement(1403..2626)
                     /locus_tag="PLH31_09320"
     CDS             complement(1403..2626)
                     /locus_tag="PLH31_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89444.1"
                     /translation="MGGLAQVRGLVEDETTDRFVRNNVLAPIVPMCAVRGPGQISFSP
                     RVLPFVWKALKATAPRRVEEAAAKCNPWDLEQGTPEVFDHLCLLAAQGLRDPQQDAFD
                     ATRGLGETEELIHCLEISHIVREALPKLSEWVSRMSGERAAAARLAYRDACVIREDSG
                     ARLFDMLAAHLPEDWRILRVISAVMDRPNDRYLASSEVSSFGERVLADIDASINLIRD
                     FDLDKGVKLSREAAMAAQRVSLQVTEFEQSVNIAKDGPWGRRLAKYKQQIAQAVELRM
                     STADRELSEALPTKPISILGKKGGKGVAKLSAPPDEALVRRATAILVFIEHLRPCATQ
                     SGYGSTRAKTLEKLNNRLDQYIEDVLYAARTGEGGDPALAQQYLDIAAGFIAHTRDDK
                     TAEIVRRRAAAAIAA"
BASE COUNT          386 a          904 c          943 g          393 t
ORIGIN      
        1 ccacgctgcc ggcctggacc aggccgccca actgcacctt gcggccggcc ggcaccttgg
       61 cttcctgggc ctgggcgggc gtgtagaaca acgagatggc gtcgcgcatg ccatacagcg
      121 ccaggccgac ggccagggcc agcacaggcg cgatggccag caggatggtc aggcgacggc
      181 gcgccttgcg ggattttggc cagaagctca ttggcggtcc cctccgggcg cggtctgctg
      241 ttggggcggc gtctgggccg cctggcgcag ggcggccaga accttgggct gatcggcata
      301 gcgccgggtg gccgatgcca tggccgcgtc gcgcttggcg acctcaccca gcaccgcata
      361 ggcccggacc agccgcaccc agccgtctgg atcgtcaggc gaggacgcca ggcgggcggc
      421 caggccatcg accatgccgg cgatcatgcc ctggacctgg gcggactgtt ccggcgcagc
      481 ctctggcgcg gggatgccgc cagcggcctc gacctgggcg atctcgcggt tcattgcgcc
      541 acggcgcgga tccccggcag gcaggtcggc ggtcagggcg cgccagtcgg caaggccgcc
      601 ggccagatca ccgtcgccaa tcttgccccg gcccagatga tagcgagccg acaggctggt
      661 ggggtcgatc ttcagggcct cacggaacgc gacctgggcg tcgccggtga cctgcccatt
      721 ggcctgcgcc acgaaggtct cgcccagacc ggtccacaga tcggcgcggc cgggggccag
      781 gcgcacggcg cggcgcaggg cctgctcggc gccggccaga tcgccggatg cgctgcgggc
      841 cagggccaga tgcttcaggg gctcggcgtc gttggggcgc tgggcggcga gggtctccag
      901 cacggcggcg atgcgcggcg ggtccagctg ggccggatcg gtggcgcgcc actcggccac
      961 gcgcctggcg aagggctggt cggaaaagcc cggcgacccg atgaagacat aggcggccac
     1021 ggccagcagc ggcgcgacgg cggcgacggc tacggccaga cgacgaggtc cggccccatc
     1081 gcgcgtccac gtctcggcgg tgtcggcggc ggccagcaga cgacgccccg cctcggcccg
     1141 cgccgccctg tgctcggcct cgtccagcag accgccttcg gccagccnnn nnnnnnngcc
     1201 ggtcgatctc gccaagctgg cgtttgtggg cgtcgagatc gggcgatacg ccctgcgttg
     1261 cgccggcgcg cgccgcgctg cgcagaacca gggcgccagc cgcagccgac agcccggccg
     1321 ccgagatcca gaaagcgatc atggccgggt gttagcttag gaaacgatcg gccgttagcg
     1381 aaaaaagcgc ttaaggggtc tctcaggccg cgatggcggc cgcagcccgg cggcgaacga
     1441 tctcggcggt cttgtcgtcc cgcgtgtgag cgatgaagcc cgctgcgata tcgagatatt
     1501 gctgggccag ggccggatcg ccgccttcgc ccgtgcgcgc cgcatagagc acgtcctcaa
     1561 tatattgatc gaggcggttg ttcagctttt ccagggtctt ggcgcgcgtc gagccatagc
     1621 cgctctgggt cgcgcagggc cgcagatgtt cgatgaacac caggatggcg gtcgcgcgac
     1681 gcaccagggc ctcatccggc ggcgcgctca gcttggccac gcccttgccg cccttcttgc
     1741 ccaggatcga gatcggcttg gtcggcaggg cctcgctcag ctcgcggtcg gccgtgctca
     1801 tccgaagttc gacggcttgg gcgatctgct gcttgtactt ggccaggcgg cgcccccagg
     1861 ggccgtcctt ggcgatgttc accgattgct cgaactcggt gacctgcagg gagacgcgct
     1921 gggcggccat ggccgcctcg cgactcagct tgacgccctt gtccaggtcg aagtcgcgga
     1981 tcaggttgat cgaggcgtcg atatcggcca gcacccgctc gccgaacgag ctgacttccg
     2041 acgaggccag ataacggtcg ttggggcggt ccatgaccgc cgagatcacc cgcaggatcc
     2101 gccagtcctc aggcagatgc gccgccagca tgtcgaacag ccgggcgcct gagtcctcgc
     2161 ggatgacgca ggcgtcgcga taggccagcc gcgccgcagc cgcccgctca ccgctcatcc
     2221 ggctgaccca ttcggacagc ttgggcaggg cctcgcgcac gatgtggctg atctcgaggc
     2281 aatggatcag ttcttcggtt tcgcccaggc cgcgggtggc gtcaaaggcg tcctgctggg
     2341 gatcgcgcag cccttgggcg gccagaagac atagatggtc aaagacttcc ggtgttccct
     2401 gttccaggtc ccagggattg cacttggcgg cggcttcctc gacgcggcgc ggcgcggtgg
     2461 ctttcagcgc cttccagacg aagggcagca cacgcggcga aaagctgatc tggccggggc
     2521 ctcgcaccgc acacatcggc acgatcgggg ccaggacgtt gttgcggacg aagcggtcag
     2581 tggtctcgtc ctcgacaagg ccgcgcacct gcgccagtcc gcccacgctg ccgacg
//
LOCUS       DAOMEO010000381         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4196_length_2633_cov_2.150892, whole genome shotgun sequence.
ACCESSION   DAOMEO010000381 DAOMEO010000000
VERSION     DAOMEO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_2633_cov_2.150892"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..692
                     /locus_tag="PLH31_09325"
     CDS             339..692
                     /locus_tag="PLH31_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR89445.1"
                     /translation="MAYTRKFSELADAARKRVDAVAPADVDRHMAAGAVVLDIRDPDE
                     HAVDHIENAINVSRGKLEMNIEEAVPDLDTTILCYCNVNNRGALSADTLRAMGYRNAK
                     YIEGGLKAYRALQST"
     assembly_gap    785..794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<795..1146)
                     /locus_tag="PLH31_09330"
     CDS             complement(<795..1146)
                     /locus_tag="PLH31_09330"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQR89446.1"
                     /translation="MDLAVLRFVGAVLLAAGLGLDVWAMVTMSRRRANILPHRAATAL
                     VTTGPFAWSRNPIYLGNTIALMGAGLAFANPWFLPAAIIAAIAVLRLAILREEAHLAM
                     QFGVAWDRYCGRTAR"
     gene            complement(1287..1652)
                     /locus_tag="PLH31_09335"
     CDS             complement(1287..1652)
                     /locus_tag="PLH31_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89447.1"
                     /translation="MSAMVAVVRDHIDLPRSEPRAPGPFAFEDPDYLRDVLETSGFRD
                     MTLEAWSADVPFAGPGATCADAVAFALDGMAFRDKVAALTKPERAKLSQALGALYDKH
                     ATPDGIMMPAKAWLVHAVV"
     gene            complement(2196..>2633)
                     /locus_tag="PLH31_09340"
     CDS             complement(2196..>2633)
                     /locus_tag="PLH31_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89448.1"
                     /translation="KTASAAVALATALATGPASAQTPSPIRLMTVITTANPEAQFMAL
                     TLLKTSGVDPRQIRVLLCGPGGDLALKVPAEASPQTFGPESRTAHALLAELDENGATI
                     EVCAIYLPARRSDRSILMDSIGVASPADIGPILADPTVRVLTF"
BASE COUNT          499 a          808 c          854 g          462 t
ORIGIN      
        1 gcaatccgat tgggaatgtc gccggatctg gctcaggcgg tcactcttcc ctttgttgtc
       61 gggctggttt ggctctacct cctgctcaaa atgcgccgtt tggatcatgc cccaagggcc
      121 cgcttgacgg gaagatcttg atgacgccgc cgctcgttat tgttcttggg gtttcgaagt
      181 gagggctcat cctctctttg ctgcgtctga tccccttggt aattagctga cgaataaaaa
      241 agtaacgtca ggagcgacaa gtgaccctac attatgatcc gcgctcttat tcatgtgctt
      301 aaatgaatga acctcaacag caatcgatag gaattcccat ggcctacact aggaagtttt
      361 cagaactcgc cgacgctgcg cgtaaacgcg ttgacgccgt cgcgccagcc gacgtcgacc
      421 gacacatggc cgccggcgcg gtcgtcctcg atattcggga tcccgatgag catgcggtcg
      481 atcacatcga gaacgcgatc aacgtcagcc gtggcaagct cgagatgaac atcgaagaag
      541 ccgtcccgga tcttgacacg accatcctct gctactgcaa cgtcaacaat cgaggggcgc
      601 tgtccgccga tacgttgcgg gccatgggct atcgcaatgc taaatatatc gagggcgggc
      661 taaaggcgta tcgtgcgcta caatcgacct aggccactgc acggctctgt cttttcagag
      721 ccggaatggc gcagtctctg atcgcgtggc ggctcaacga tggctgttcc ggccgaacca
      781 gcgcnnnnnn nnnnagcgcg ccgttcgtcc gcaatagcga tcccaggcta cgccgaactg
      841 catggccaga tgagcttcct cgcgtaggat cgccagacgc aggaccgcaa tcgccgcgat
      901 gatcgcagcg ggcaagaacc acgggttggc gaaggccagg ccggctccca tcagagcgat
      961 cgtatttccc agatagatcg ggttcctgga ccaggcgaac gggcccgtcg tgaccagggc
     1021 cgtggcggcg cgatggggca ggatgttggc gcgtcggcga gacatcgtaa ccatcgccca
     1081 gacatcgagc ccgaggcccg ccgccagaag cacggcgccc acgaagcgca ggacggccag
     1141 gtccatttcc ggccgtagcg gccaaatgga gttcgcagcg acagcaagaa gggtcgcggc
     1201 gacatagatc atcgggggcc acgggagccg gttcggagcg gagaccgggc cgtcgtgtcg
     1261 ctgccgtaag ttgggatcat cacgcatcag acgacagcat gcactaacca agccttggcc
     1321 ggcatcatta tcccgtctgg cgtggcgtgt ttgtcgtaca gggcgccgag cgcctgcgag
     1381 agctttgccc gctctggctt cgtgagcgcc gcgaccttgt ctcgaaaggc catgccgtcc
     1441 agggcgaagg ccacggcgtc ggcgcatgtg gcgcctggtc cagcgaaggg aacgtccgca
     1501 ctccaggcct caagggtcat gtcccggaaa ccggatgtct cgagtacgtc ccgcagatag
     1561 tccggatcct cgaaggcgaa aggaccgggg gctcgcggct ctgaccgggg aaggtcgata
     1621 tgatcgcgca cgaccgcgac catggccgac atccagaggt tctcgcgggg ggccgcccag
     1681 accgcgagat cgaagcggcc gccgggatga atcgcgccgt gcagatcccg gaggcgcgta
     1741 gggctccgca aagaacatac tgccgaaccg ggaaatcacg cggtcgaaga cgcgcccttc
     1801 aggctgttcg ccggcggcgt cggcgcaccg gaaactgaca ttcgacatgc ccgccctctc
     1861 ggcccgctgg cgggctcggg cgatgaggtc gggcgatatg tcgagaccga gcgcggtgcc
     1921 cagagggccg accgcgcatc cggccgccaa agtggtgacc ccgcctccac aacccacgtc
     1981 gaggaccctc tggccggcgg tcaggctcgc gcggttcatc aaggccgtcc cgatcggagc
     2041 gatcatgccc tcgaacgtct tgatgtgggc aagccaccgc gcggccattt cgccggccca
     2101 gttttcggcc gggtcggtga tgagaggcgt cacggtctcg ttcatgggga ggacctcgat
     2161 cgtgtctgtg tgacgaacgg ttcgcccaat ggcgcttaga aggtcagtac ccggacggtg
     2221 gggtccgcaa ggatgggtcc tatatcggcc ggagacgcca caccgatcga atccatcagg
     2281 atagatcgat ccgagcgcct tgccggcaga tagatggcgc agacctctat ggtcgccccg
     2341 ttctcgtcca actccgctag cagcgcgtgg gccgtgcgcg actcggggcc aaatgtctgc
     2401 ggagacgcct ctgcggggac cttcagcgcc agatcaccgc ctggcccgca gagcagaaca
     2461 cggatttggc ggggatcgac gccgctcgtc ttgagcaggg tcagcgccat gaactgggcc
     2521 tcgggattgg cggtggtgat gacggtcatc aggcggatcg gcgacggcgt ttgagccgaa
     2581 gccggtcctg tggcgagcgc tgtcgccagc gcgactgcgg cggaggcggt ctt
//
LOCUS       DAOMEO010000382         2631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4198_length_2631_cov_2.309394, whole genome shotgun sequence.
ACCESSION   DAOMEO010000382 DAOMEO010000000
VERSION     DAOMEO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4198_length_2631_cov_2.309394"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLH31_09345"
     CDS             <1..234
                     /locus_tag="PLH31_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HQR89449.1"
                     /translation="VSVCGEMAGRPLEAFALVALGFDRLSMPPAGVGPVKQMVLSCDR
                     EAARRNVEALLKGSGGSIRGEIETLARKLYVAV"
     gene            391..>842
                     /locus_tag="PLH31_09350"
     CDS             391..>842
                     /locus_tag="PLH31_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89450.1"
                     /translation="MPLDTGSVRHLHLVADAEPEEGFASFDQPSVDEGVDIGAALRAA
                     RTFRGLTTQDVADATRIRQSYIDALEDLRLEELPSRPFTIGYVRAYATLLGLDGEAAV
                     LRFKNDVPDEGGELRAPVGVRHERDPRLALILIAGMLMVGAILLWNVA"
     assembly_gap    843..852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <853..1371
                     /locus_tag="PLH31_09355"
     CDS             <853..1371
                     /locus_tag="PLH31_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89451.1"
                     /translation="APPAALAPVGSAVSLGAPLPAPVESTTPEPYKTPGLDDAAANGG
                     SADAANAASKARATALAAGEGEIDAAHVAALGSSFKPRGVVHGAAAADSSGVILLARK
                     AATLVVRGADGSVYFARQLNAGEAFRAPRTAGLTADVSDPSNFDVYAGGVLTGRLTAS
                     TTALSKLVKSAA"
     gene            1452..2591
                     /gene="ispG"
                     /locus_tag="PLH31_09360"
                     /gene_synonym="gcpE"
     CDS             1452..2591
                     /gene="ispG"
                     /locus_tag="PLH31_09360"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQR89452.1"
                     /translation="MAADHTHIRPWRSIERRKSRKIRVGNVEVGGDAPITVQSMTNTL
                     TSDAAATLEQIRQLEEAGADIVRVSCPDTDSTAAFKTIARESRVPLVADIHFHYKRGI
                     EAAQAGAACLRINPGNIGSADRVRDVIQAARDHGCSMRIGVNAGSLERELLEKYGEPC
                     PDAMVESALNHARILQDHDFHEFKISVKASDPFMTVAAYHQLAERIDCPLHLGVTEAG
                     ALRTGTVKSSIGIGSMLWAGIGDTIRVSLAADPVEEIKVGFDILKSLGLRHRGVNIIA
                     CPSCARQGFNVIKTVEALEERLAHISQPMSLSIIGCVVNGPGEALMTDLGFTGGGAGS
                     GMIYMAGKPDHKQSNDGMIEHIVELVEKRAAELKAAQDAEAIAAE"
BASE COUNT          421 a          872 c          861 g          467 t
ORIGIN      
        1 gtctctgtct gcggcgaaat ggccggtcgg ccgctggagg ccttcgccct ggtggccctg
       61 ggctttgatc gactgtcgat gccgccggct ggcgtcgggc cggtgaagca gatggtgctg
      121 tcctgcgacc gtgaagccgc caggcgcaac gttgaggccc tgctcaaggg atcgggcgga
      181 tcgatccggg gagagattga aaccctggcg cgcaagcttt acgtcgcggt ttgatttggt
      241 ggtcgattaa ccaatcttcg ctgtttagtg tattgaatac cgctacttac tttgatatcc
      301 acaaagggtt gttaagaact tcgtgcaaga cgaagtgggg tggggcgtcg cgttcgcgcg
      361 ttacgcgttt tttggggtga tatagtcgca atgccgctgg atacgggcag tgtcaggcac
      421 ttgcatctgg tcgcggacgc tgagcctgaa gagggctttg cgtcgttcga ccagccgtca
      481 gtcgacgagg gcgtcgatat tggcgctgcg ctccgggccg cccgcacctt ccgcggcctg
      541 accacccagg acgtggccga cgccactcgt attcgtcaaa gctatatcga cgcgcttgag
      601 gacctgcggc tggaagagct gccctcgcgt ccgttcacca tcggctatgt ccgcgcctac
      661 gccacactgc tggggctgga tggcgaggcc gctgttctgc gcttcaagaa cgacgttccc
      721 gatgagggcg gcgagctgcg tgctccggtc ggcgtgcgcc acgaacgtga tccgcgcctg
      781 gccctgatcc tgatcgccgg catgctgatg gtcggcgcca tcctgctctg gaacgtggcg
      841 cannnnnnnn nngccccgcc cgcggccctg gctccggtcg gttcggcggt gtcgcttggg
      901 gctcccttgc cggcgcccgt cgagtcgacc acgcccgagc cgtacaaaac gccgggtctc
      961 gatgacgccg ccgccaatgg tggctcggct gatgcggcca acgccgcctc aaaggctcgc
     1021 gccacggctc ttgcggcggg tgaaggcgag atcgacgccg cgcatgtcgc ggccctgggc
     1081 tccagcttca agccgcgcgg cgtggtgcat ggcgcggccg ccgccgacag ctccggcgtc
     1141 attctgctgg cgcgcaaggc cgctaccctg gtggttcgcg gcgccgatgg ctcggtctat
     1201 ttcgcccgcc agctcaatgc cggtgaagcc ttccgcgccc cgcgcaccgc tggcctgacc
     1261 gccgatgttt ccgaccccag caatttcgac gtctatgcgg gcggcgtgct gaccggccgc
     1321 ctcacggcgt cgaccaccgc gctgagcaag ctggtcaagt ctgcagcctg atccggagcg
     1381 ccggtccgga accggcgcga agcttccatg acagccttgg gttctccgcg caaaagccct
     1441 agattgcccc catggccgca gaccacaccc atatccgccc ctggcgctcc atagagcgtc
     1501 gcaagtcgcg caagattcgt gtcggcaatg tcgaggtcgg gggcgacgcg cccatcaccg
     1561 tccagtcgat gaccaacacc ctgaccagcg acgccgccgc gacgctggag cagatccgcc
     1621 agctggaaga ggccggcgcc gacatcgtcc gcgtcagctg ccctgacacc gacagcacgg
     1681 ccgcgttcaa gaccatcgcc cgtgaaagcc gggtgccgct cgtggccgat atccacttcc
     1741 actacaagcg cggcatcgag gcggcccagg ccggcgcggc ctgcctgcgg atcaatccag
     1801 gcaatatcgg ctcggccgac cgggttcgcg acgtgatcca ggctgcccgc gaccacggct
     1861 gctcgatgcg gatcggcgtc aatgccggct cgctggagcg cgagctgctg gaaaagtacg
     1921 gcgagccgtg ccccgacgcc atggtcgaga gcgcgctcaa ccacgcccgc atcctgcagg
     1981 accacgactt ccacgagttc aagatttcgg tgaaggcgtc ggatccgttc atgacggtgg
     2041 cggcctatca ccagctggcc gagcgtattg attgtccgct gcaccttggc gtcaccgagg
     2101 ctggcgccct gcgcaccggc acggtgaaat cctcgatcgg cataggctcg atgctgtggg
     2161 ctggcatcgg cgataccatc cgggtctcgc tggccgccga cccggtggaa gagatcaagg
     2221 tcggctttga catcctcaag tcgctgggtc tgcgccatcg cggcgtcaac atcatcgcct
     2281 gcccgtcctg cgcccgtcag ggtttcaacg tcatcaagac ggtggaagcc ctggaagagc
     2341 gtctggccca catcagccag ccgatgtcgc tgtcgatcat cggttgcgtg gtcaacggcc
     2401 cgggcgaggc cctgatgacc gacctcggtt tcacgggcgg cggcgcgggc tcgggcatga
     2461 tctatatggc cggcaagccc gaccacaagc agtccaacga cggcatgatc gagcacatcg
     2521 tcgagctggt ggaaaaacgc gcggccgagc tgaaggcggc tcaggacgcc gaggccatcg
     2581 ccgcggagta ggcttcagcc cagcgaggtc tggatcggta cgccgccctt t
//
LOCUS       DAOMEO010000383         2627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4203_length_2627_cov_2.404743, whole genome shotgun sequence.
ACCESSION   DAOMEO010000383 DAOMEO010000000
VERSION     DAOMEO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_2627_cov_2.404743"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLH31_09365"
     CDS             <1..1145
                     /locus_tag="PLH31_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HQR89453.1"
                     /translation="RRIWPSLAALVLGGACSLVGGFDPVGLGLGAAVGVLCWAALGSR
                     VGVLGVWIGGLVTLLLAALAAQILAPGATVMLVWPLLIAALGATLAMALGGTRDRRVA
                     LAMTLAMAVLATLTIAQLTAWGGWTFAGIGLMEPAVLALFVLLATPALVPITFDFAES
                     RWGWRGVVLLGVAGLGLVITAAIPNASADRPNLTEAHHIADPSTGKAWRVSSMPELDA
                     WTRQVLVADGGVNLKKQALEPFHAEPIWMAQTRAVPVTPPVLTAERSGDRLIVRVIPG
                     PGTEVLTLKIRATTGLHDPRLNGRPITLTSQPDAWSTVTYHAPDPNGVTLSFTSEATG
                     RVEAAVVEMRDGWPAEAQPPAPKPPELMAVGMSDKTAVIAHARLAW"
     gene            complement(1347..>2215)
                     /locus_tag="PLH31_09370"
     CDS             complement(1347..>2215)
                     /locus_tag="PLH31_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR89454.1"
                     /translation="EAMENDPVLRASFFKNLRYMGYGGATLSNDVYTRIQALAVAETG
                     HRIPLTTMYGATETQGITVTHWITERVGLVGLPLPGIQLKLAPNGSKFEVRVKGPTVA
                     AGYHKDPEKTAAAFDEEGFYCLGDAARFVDPDDPAKGLVFDGRVTEDFKLDSGTWVSV
                     GVLRPDLVAACSPYIADAVICGQDKGYVGALFWPSPSGLMALVADPGPGTPMDKLVAF
                     LKERLAAFNASAGGSSRRIGRFTVMTEPPSIDAGEITDKGYVNQRATLERRAALVEAL
                     YAAEPGEGVVVV"
     assembly_gap    2216..2225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2226..>2627)
                     /locus_tag="PLH31_09375"
     CDS             complement(<2226..>2627)
                     /locus_tag="PLH31_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR89455.1"
                     /translation="GDGAVSFDDIAATVPTLAVEASRETLGPATVAKYLFTSGSTGMP
                     KAVAQTHGMMAGVIAGQDGLKLDEPDDGEVSQGLEWMPWSHISAGNISFNGLLWAGGT
                     LHIDEGKPLPGMFETTIKNLYEVSPIVFGSAP"
BASE COUNT          383 a          949 c          865 g          420 t
ORIGIN      
        1 ccaggcggat ctggcccagc cttgcggctc tcgtcctcgg cggcgcctgc agcctggtcg
       61 gcgggttcga tcctgtaggc ttgggcctcg gcgccgcggt cggcgttctg tgctgggcgg
      121 ccctgggaag cagggtcggt gtcctgggcg tctggatcgg cgggctcgtc accctgctgc
      181 tcgccgccct ggccgcccag atcctggccc ccggcgcgac ggtgatgctg gtctggccgc
      241 tactgatcgc cgccctgggc gcgaccctgg ccatggccct gggcggcacc cgcgaccgtc
      301 gcgtggccct ggccatgacc ctggccatgg cggtgctggc caccctgacc atcgcccagt
      361 tgaccgcctg gggcggctgg accttcgccg gcatcggcct gatggagccc gccgtcctgg
      421 cgctcttcgt cctgctggcc accccggccc tggtgccgat cacctttgac ttcgccgagt
      481 ctcgctgggg ctggcgcggg gtggtgctgc tgggcgtcgc cggtctgggc ctggtcatca
      541 ccgccgccat tcccaacgcc tcggctgacc ggcccaacct gacagaggcc catcacatcg
      601 ctgatccttc aacgggcaag gcctggcggg tctcgtcaat gccggagctg gacgcctgga
      661 cccgccaggt cctggtcgcc gacggtgggg taaacctgaa aaagcaggcc ctggaaccgt
      721 tccacgccga gccgatctgg atggcgcaga ctcgcgccgt gccggtcacc ccgccggtcc
      781 tgaccgccga gcgctcgggc gaccgcctga tcgtccgcgt cattcccggt ccgggaaccg
      841 aggtgctgac cctgaagatc cgcgccacca ccgggctgca cgatccgcgc ctgaacggcc
      901 gaccgatcac cctgaccagc cagcccgacg cctggtcgac cgtgacctat cacgcccctg
      961 atcccaatgg cgtcacgctc agcttcacgt cggaggcgac cggccgggtc gaggcggccg
     1021 tggtagaaat gcgcgacggc tggcccgccg aggcccagcc ccccgcgccc aagccgccgg
     1081 agctgatggc cgtcggcatg tcggacaaga ccgccgtgat cgcccatgcg cggctggcgt
     1141 ggtaggggac aaccgagttc aaaaactcaa taacctcgtc ttcgtgggcc ttgtgcccag
     1201 gacccatgcc ggcggagcgc tttgcgcttc agggtcagtg gaagccgaca ggcccggcgc
     1261 gccttccccc tcaaagccag ctgaaccatg gatcctgggc acaaggccca gaaaggcggg
     1321 ttttgtggat gaagagggcg ttggccctaa actaccacca cgccctcccc cggctccgcc
     1381 gcatacagcg cctccaccag cgccgcgcgc cgctccagcg tcgcgcgctg gttcacataa
     1441 cccttgtcgg tgatctcgcc ggcgtcgatc gagggcggct cggtcatcac ggtgaagcgg
     1501 ccaatccggc gcgacgagcc gccggcgctc gcattgaacg ccgccagccg ctccttcaaa
     1561 aacgccacca gcttgtccat cggcgtgccc ggccccggat cggcgaccag ggccatcagc
     1621 cccgacggcg agggccagaa caaagcgccg acatagccct tgtcctgacc gcagatcacc
     1681 gcgtcggcaa tataggggct gcaggcggcc accaggtcgg ggcgcagcac gccgacgctg
     1741 acccaggtgc cgctgtcgag cttgaagtcc tcggtcaccc ggccgtcgaa caccagcccc
     1801 ttggccgggt cgtcgggatc gacgaaccgc gccgcgtccc ccaggcagta gaaaccttcc
     1861 tcgtcgaagg ccgccgcggt cttttcggga tccttgtgat agcccgccgc cacggtgggc
     1921 cccttgaccc gcacctcgaa cttggacccg ttgggggcca gtttcagctg gatgcccggc
     1981 aggggcagac ccaccagccc gacccgctcg gtgatccagt gggtgacggt gatgccctgg
     2041 gtttcagtcg cgccgtacat cgtggtcagc ggaatgcggt gacccgtctc ggccacggcc
     2101 agggcctgga tgcgggtata gacgtcgttg gacagcgtcg ccccgccata gcccatatag
     2161 cgcaggttct tgaagaacga ggcgcgcagc accgggtcgt tctccatcgc ctcggnnnnn
     2221 nnnnncgggg ccgagccgaa cacgatgggc gagacctcat agaggttctt gatcgtggtc
     2281 tcgaacatcc ccggcagggg cttgccctcg tcgatgtgca gggtgccgcc ggcccagagc
     2341 agaccgttga agctgatatt gccggccgag atatggctcc acggcatcca ttccaggccc
     2401 tgcgagactt cgccgtcgtc gggctcgtcc agcttcagcc cgtcctggcc ggcgatcacc
     2461 ccggccatca tgccatgggt ctgggccacg gccttgggca tgccggtcga gccggaggtg
     2521 aacaggtact tggccaccgt ggccggcccc agcgtctcgc gcgaggcctc gacggcgagg
     2581 gtcgggacgg tggcggcgat gtcgtcaaag ctcaccgcgc cgtctcc
//
LOCUS       DAOMEO010000384         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4215_length_2620_cov_3.195322, whole genome shotgun sequence.
ACCESSION   DAOMEO010000384 DAOMEO010000000
VERSION     DAOMEO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_2620_cov_3.195322"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="fsa"
                     /locus_tag="PLH31_09380"
     CDS             complement(<1..625)
                     /gene="fsa"
                     /locus_tag="PLH31_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQR89456.1"
                     /translation="MQIFLDSTDTKVIADLASTGLVDGVTTNPTLIAKAGRPMLEVIA
                     EICDLVPGPISAEVAATTADAMIAEGQKLAGIAPNVVVKIPLTRDGLIACAAFANEGI
                     STNVTLCFSPTQALLAAKAGATFVSPFIGRLDDYGFDGMDLIRDIRTIYDNFGYETEI
                     LAASVRNVAHVKDAAIVGADVVTIPPAVFGDLFKHPLTDKGLDQFLKD"
     gene            complement(704..1585)
                     /gene="rfbD"
                     /locus_tag="PLH31_09385"
     CDS             complement(704..1585)
                     /gene="rfbD"
                     /locus_tag="PLH31_09385"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQR89457.1"
                     /translation="MIPKVLQFGATGQVGQALVAAAGRAVTLTVLSRAEVDLTDAAAI
                     RAAIRAANCDLVWNAAAFTLVDRAEAEPDAAFAVNAAAPAAMAAACAERDLPFVHLST
                     DSVFNGQTGRPYVETDTAEPISVYGRSKLAGEQGALAHPRTTVLRVSWVFSPYGRNYV
                     SFMLNLGRTREVLQVVADQFGSPTDGEALAGFMVANAARWAAASADDPAFGLFHFANA
                     GETSRYDFARAAIARDPLTRARLEPTTQGAFVEAAARPPRSPLDSSKLAATFGFTPQP
                     WKPAVERVADQLVRNGI"
     gene            complement(1582..2118)
                     /locus_tag="PLH31_09390"
     CDS             complement(1582..2118)
                     /locus_tag="PLH31_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR89458.1"
                     /translation="MDTLPPLRAVFFDRDGVLNVDHGYVADPARLNWIDGAREAVAAL
                     TKAGLKVMVVTNQSGIGRGYFEEAAMHGFHAEMQAQLEPLGGRIDAFYFAPHHENAAI
                     DAYRVTDHPDRKPNPGMVLRGLAEFGLSPAEAVIIGDRSSDVEAGRRAGMPGYLFRGG
                     DLRAFVLSVLGDRVPGLK"
     gene            2220..>2620
                     /locus_tag="PLH31_09395"
     CDS             2220..>2620
                     /locus_tag="PLH31_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQR89459.1"
                     /translation="MTDAFTDAVRLRDLLKTWANHAAFPLWWKVGADHKGGGFFEKIE
                     QSGEAPDSPRRGRVLPRQIYSFTAAGKLGWTGPWKKAVEHGLSYYLDVYRKPDGLFRT
                     LVGPGGVVLDDTAPLYDQAFALFALANAFEVT"
BASE COUNT          413 a          825 c          952 g          430 t
ORIGIN      
        1 agtccttcag gaactgatcc aggcccttgt cggtcagcgg gtgcttgaac aggtcgccga
       61 ataccgccgg cgggatggtg acgacatcag cgccgacgat ggcggcgtcc ttgacgtggg
      121 cgacgttgcg caccgaagcg gccaggattt cggtctcgta gccgaagttg tcgtagatgg
      181 tgcggatgtc ccggatcaga tccatgccgt cgaagccata gtcatccagg cgtccgatga
      241 acggcgagac gaaggtggcg ccggccttgg cggccagcag ggcctgggtc ggcgagaagc
      301 acagggtgac attggtggaa atgccttcat tggcgaaggc ggcgcaggcg atcaggccgt
      361 cgcgggtcag cgggatcttc acgacgacgt tcggcgcgat gccggccagc ttctggccct
      421 cggcgatcat ggcgtcggcg gtggtggcgg cgacttcggc gctgattggg ccgggcacca
      481 ggtcgcagat ctcggcgatc acttccagca tcggacggcc ggccttggcg atcagggtcg
      541 ggttggtggt cacgccgtcg acgaggccgg tggaggccag atcggcgatg accttggtgt
      601 cggtgctgtc gaggaagatc tgcatgggac ttcttgcgct gaattttttg ggagttcggg
      661 ccggtttatc gcgacgggcg gccgcgcgcc agcattggcg acttcaaatg ccgttacgga
      721 ccagctggtc ggcgacccgc tcgacggccg gtttccaggg ctggggcgtg aaaccgaagg
      781 tcgcagccaa tttcgaactg tccagcggcg agcgcggcgg gcgggcggcg gcctcgacga
      841 aggccccctg ggtcgttggc tcgaggcggg cgcgggtcag cggatcgcgg gcgatggcgg
      901 ccctggcgaa gtcgtagcgg ctggtctcgc cggcattggc gaagtggaac aggccgaaag
      961 cggggtcatc ggctgacgcg gcggcccagc gcgcagcgtt ggcgaccatg aagcctgcca
     1021 gcgcctcgcc gtcggtcggg ctgccgaact ggtcggcgac gacctgcagc acctcgcgcg
     1081 tgcggcccag attgagcatg aacgacacat agttgcggcc atagggcgag aacacccacg
     1141 aaacccgcag caccgtcgtc ctcggatggg ccagcgcccc ctgctcgcca gccagcttgg
     1201 agcggccata gacgctgatc ggctcggcgg tgtcggtctc gacatagggg cggcccgtct
     1261 ggccgttgaa gacgctgtcg gtggagagat ggacaaaggg caggtcgcgt tctgcgcagg
     1321 ccgcggccat ggctgctggc gcggccgcgt tgaccgcgaa ggcggcgtcc ggttcggcct
     1381 cggccctgtc caccagggtg aaagcggcgg cgttccagac caggtcgcag ttggcggcgc
     1441 ggatcgccgc gcggatcgcc gccgcgtccg tcaggtcgac ttcggctcgc gacaggacgg
     1501 tgagggtcac ggcgcggcct gcggccgcca ccagcgcctg tccgacctga ccggtcgcac
     1561 cgaactgcag gaccttgggg atcacttcag gcccggcacg cggtcgccca gcaccgagag
     1621 gacaaaggcg cgcaggtcgc cgccccggaa cagatagccc ggcatgccgg cgcggcggcc
     1681 ggcttcgacg tcgctggacc ggtcgccgat gatcaccgcc tcggcggggc tcaggccaaa
     1741 ctcggccagg cctcgcaaga ccatgccggg attgggcttg cggtcgggat ggtcggtgac
     1801 ccgataggcg tcaatcgccg cgttctcgtg atggggggcg aaatagaagg cgtcgatccg
     1861 gccgcccagc ggttcgagct gggcctgcat ctcggcgtgg aagccatgca tggccgcttc
     1921 ctcgaaatag ccccggccga tccccgactg gttggtgacc accatcacct tcaggcccgc
     1981 tttcgtcagg gcggccacgg cctcgcgggc gccgtcgatc cagttcagtc gggccggatc
     2041 ggcgacatag ccatgatcca cattgagcac gccgtcccga tcgaagaaga cggcgcgaag
     2101 ggggggcagg gtgtccatag gtcgagcttt atcgcctgtc tgggcgctac aacaagtcgc
     2161 tgtcttggcg cgcccccgcg acttgcccta gaaaacagcc ctcagccgga gcgccccaga
     2221 tgactgacgc cttcaccgac gccgttcgcc tacgcgacct gctgaagacc tgggccaacc
     2281 acgccgcctt tccgctgtgg tggaaggtgg gcgccgacca caagggcggc ggctttttcg
     2341 agaagatcga gcagtccggc gaggcgccgg attcgccgcg tcggggccgg gttctgccgc
     2401 gccagatcta ttcgttcacc gcggcgggca agctgggctg gaccgggccg tggaagaagg
     2461 ccgtcgagca cggcctgagc tattatctgg acgtctatcg caagcctgat ggcctgttcc
     2521 gcaccctggt cgggccgggc ggagtggtgc tggacgacac cgccccgctc tacgatcagg
     2581 cgtttgcgct gtttgctttg gccaacgctt tcgaggtgac
//
LOCUS       DAOMEO010000385         2619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4219_length_2619_cov_1.998440, whole genome shotgun sequence.
ACCESSION   DAOMEO010000385 DAOMEO010000000
VERSION     DAOMEO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4219_length_2619_cov_1.998440"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>286)
                     /locus_tag="PLH31_09400"
     CDS             complement(<1..>286)
                     /locus_tag="PLH31_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQR89460.1"
                     /translation="FNDDVAGENLKFLIMVWQALGDERVLPAIRRAMDCFLACQQPMP
                     QPAWGLQHKVSDLKPAGARTYEPEAFATHTTGGNIASCMDFYELSGDPKFL"
     assembly_gap    287..380
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<381..1080)
                     /locus_tag="PLH31_09405"
     CDS             complement(<381..1080)
                     /locus_tag="PLH31_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQR89461.1"
                     /translation="MPLSPISRRRLMATSAAAFAVAGCASPLKALAAAPPTKAQALET
                     MKKATTFMVEKAAYNGGYVWSYLPDFSRRWGEMEAFPTMIWVQPPGTATMGHLFLDAY
                     HATGDAYYYEAAVKAAQALIAIQHPAGGWNYLGDLAGEASLKKWYDTIGKNGWRLEEF
                     QHYYGNATFDDAGTAESSQFLLRLYVEKQDPAFKPALDKALAFVLDSQYPIGGWPQRF
                     PLRDEFHHHGKADYT"
     gene            1547..1726
                     /locus_tag="PLH31_09410"
     CDS             1547..1726
                     /locus_tag="PLH31_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89462.1"
                     /translation="MPTRTEHIHEAERLERQAEIADNAHARAALRRMAQASRGAAALV
                     GMFEASDEDCSLARL"
     gene            2038..2598
                     /locus_tag="PLH31_09415"
     CDS             2038..2598
                     /locus_tag="PLH31_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89463.1"
                     /translation="MTPRSLLVAGVLTATLFTAVGATASTLVLGGGAAKDCSVAAIDG
                     RSDKTSVLLCTTALETETLSFRDRARTYVNRGVLQMRQRDFPTARADFDAASRIDPDL
                     GEAWVNRGAAYVGEERYSDALGQIDKGLALGVKDPEKAWFNRAMAHEGLGDLKAAYQD
                     YSRAAELDPEWDAPKRELMRFTVKRP"
BASE COUNT          396 a          768 c          898 g          463 t
ORIGIN      
        1 aggaacttcg ggtcgccgct cagctcatag aaatccatgc acgaggcgat gttgccgccg
       61 gtggtgtggg tggcgaaggc ttcgggctca taggtgcggg cgccagcggg cttcaggtcg
      121 gaaaccttgt gctgcagacc ccaggccggt tgcggcatgg gctgctggca ggcgaggaag
      181 cagtccatgg ctctgcggat ggcgggcagc acgcgttcgt cgcccagggc ttgccacacc
      241 atgatcagga acttgaggtt ttccccggcc acgtcgtcat tgaaggnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn cggtgtagtc ggccttgccg tgatggtgga actcgtccct
      421 cagcgggaag cgctggggcc agccgccgat cgggtactgg ctgtccagga cgaaggccag
      481 ggccttgtcg agggccggct tgaaggcggg gtcctgcttt tcgacataca ggcgcagcag
      541 gaactgtgag ctttcggccg tgccggcgtc gtcgaaggtg gcgttgccgt aatagtgctg
      601 gaattcctca agccgccagc cgttcttgcc gatggtgtcg taccactttt tcagcgaggc
      661 ctcgccggcc aggtcgccca gatagttcca gccgccggcc ggatgctgga tggcgatcag
      721 ggcctgggct gccttgacgg cggcctcgta gtaataggcg tcgccggtgg cgtgataggc
      781 gtccaggaac aggtggccca tggtggccgt gccgggcggc tgcacccaga tcatcgtcgg
      841 gaaggcctcc atctcgcccc agcggcgcga gaagtcgggc aggtagctcc agacgtaacc
      901 gccgttatag gcggctttct cgaccatgaa ggtggtggcc ttcttcatgg tctccagggc
      961 ctgggccttg gttggcggcg cggcggccag ggccttgagc ggcgaggcgc agccagcgac
     1021 ggcgaaggcg gcggccgatg tcgccatcag gcgacggcgg gaaatagggc taagaggcat
     1081 gcggcgctcc gactggcgtt atctcggcag gttcggggcg gcgggtcgcc cgacgctggt
     1141 cccctgaccg agcgcgcgag gcggttccgt ggtctcctcc cggaccggca aagccgatct
     1201 caccaccttc aggcggcgaa gtcttggcgc agttgtgaca ccggtgtcac gtcatggcta
     1261 ggcgcttttt tccgacaacg gcgttcgaag ccggcgggtg cgacagggcg tcgccttgcg
     1321 gtgtgatcag cgttcggcga agaccagtga ctgtttctgc ggcgccgccg ccgcgccgcc
     1381 ctggctgcgc gcgacgccgt cctgttgggg aacaggatga cgcccccgga cccgcccggt
     1441 tcgggggcgt tttttatcgt attgtaattg ttagataagt ttcgataacg ccgtgcgtcc
     1501 agcggtcgca tggcgccaaa ttttacattc tgtaaacatt cagctcatgc ccacgcgcac
     1561 cgaacatatc cacgaagccg aacggctgga acgtcaggcg gagatcgccg acaacgccca
     1621 tgcccgcgcg gccctgcgcc gcatggccca ggcctcgcgt ggcgccgcgg ccctggtggg
     1681 gatgtttgag gcgagcgacg aggattgcag cctggcgcgc ctctaggcgc gagcgcgctc
     1741 gtgttgacct cggaggcagg ttggacgcct gcgcccggag ccatgatcgg gcgccctggg
     1801 gcaggggctt agccgcgctg gatgtccagc ttgccggtct tggcgtcgcc atcgatccgg
     1861 gcgatccctg aatttgcctc gcggttctgt tagcgcatgg cgaaacacgg gcgtattatc
     1921 catatcgatg atgaggccgg gcccggcagg tccggctccg ttgtggagcg cggctcggcg
     1981 tgtctggcgg ttctgtcgcc tgacgcccag aagaaccgcg acgtcaggga ggaagacatg
     2041 acgccaagaa gccttctggt cgctggtgtg ttgaccgcga cccttttcac cgccgtcgga
     2101 gcgacggcct cgaccctggt gcttggcggc ggcgccgcca aggattgctc ggtcgccgcc
     2161 atcgacggcc gctcggacaa gacctcggtt ctgctgtgca cgaccgcgct ggagaccgag
     2221 accctgagtt tccgcgatcg ggcgcgcacc tatgtgaacc gcggcgtgct gcagatgcgc
     2281 cagcgagact tccccacggc gcgcgctgat tttgacgccg cctcccggat cgatccggat
     2341 ctgggcgagg cctgggtcaa tcgcggggcg gcctatgtgg gcgaggagcg ctatagcgac
     2401 gcccttggcc agatcgacaa gggtctggcg ctgggcgtga aggatccgga aaaagcctgg
     2461 ttcaatcggg ctatggccca cgagggactg ggcgatttga aggcggctta tcaggactat
     2521 tccagggcgg ccgagctgga tcccgaatgg gatgcgccga aacgggaact tatgcgtttc
     2581 acggtcaagc ggccctgagc gcaacgcgct ccgagcccc
//
LOCUS       DAOMEO010000386         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4228_length_2615_cov_2.714844, whole genome shotgun sequence.
ACCESSION   DAOMEO010000386 DAOMEO010000000
VERSION     DAOMEO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_2615_cov_2.714844"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLH31_09420"
     CDS             complement(<1..390)
                     /locus_tag="PLH31_09420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQR89464.1"
                     /translation="MTSRTPWAEKRFQALCAVAMVAALSLSISAPASAQDGPSLIRDT
                     EIEEILHRDADPLFEAAGLDSKTVRILLVGDKSLNAFATQGLMMGLNTGLILQTETPN
                     QLKGVIAHEAGHLAGGHPIRSAEMTRAG"
     assembly_gap    402..411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(539..>2615)
                     /locus_tag="PLH31_09425"
     CDS             complement(539..>2615)
                     /locus_tag="PLH31_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQR89465.1"
                     /translation="PQGMGLIIRTAGAKRTKAEIKRDYDYLLRLWENIRETTLHSVAP
                     ALIYEEEDLVKRAIRDMYDKELEGIWVEGEAGFREARDFMRMLMPSQAKKVFHYREPA
                     PLFVKHRIEDHLAQIYSPVVPLRSGGYLVINQTEALVAIDVNSGKATRERNIEATALK
                     TNTEAAEEAARQLRLRDLAGLIVIDFIDMDEGKNNRSVEKVLKDALKDDRARIQMGKI
                     SGFGLMEISRQRRRTGVLEGTTHVCEHCEGTGRVRSVESSALAALRAVEVEALKGSGA
                     ITLKVSRAVGIYILNEKRAYLVRLQQTHGLFVTVLVDESLHAAENEIERTELGERIAI
                     TAPPVSDDDFADDVYDDEDEDEDEEIFDEEAIEREDTDDDAAAARAAPRGKPRVEAAE
                     EEGNRKRKRRRRGRRDDADAAPEGEAAEDADGDEDSDADRADFPALEGEDEEARRRRR
                     RRGRRGGRRGGREDGDRPADAFVWTRPRVPFGDSAFAWHDPAALDGRGGESRRPAPEQ
                     AAPAPQAERPERAERPERAERSDRSEGRSSRSRGRGRGEREARPAPVDTPVDVELPPI
                     PAVIAEAPPSDIWVELPAPSDEAPKKARRPRTRGRKGDTPAADTMIETVAEAVVAPEV
                     VAEPVAVEAPTEIEVEVPAPAPIAAPEPQPEPEAELVAEQALAPAEPDPAEITAPPAK
                     PRGGWWRRG"
BASE COUNT          367 a          853 c          890 g          495 t
ORIGIN      
        1 gcccgcgcgc gtcatttccg ccgagcggat ggggtggccg ccggccaggt gtccggcttc
       61 gtgggcaatg acgcccttga gctggttggg cgtctcggtc tgaaggatca ggccagtgtt
      121 gaggcccatc atcaggccct gggtcgcgaa ggcgttcagc gatttgtcgc cgaccagcag
      181 gatacgaacc gtttttgagt cgagcccggc cgcttcaaac agcgggtctg cgtcacgatg
      241 caggatttcc tcgatctcgg tgtcgcggat cagcgacgga ccatcctggg cggaggccgg
      301 cgccgatatg gaaaggctga gcgccgcgac catggccacg gcgcagaggg cttgaaacct
      361 tttttcagcc cacggcgtgc gggaggtcat tcaacgtctc cnnnnnnnnn ngcccccgcg
      421 atcgtggcaa gtgtagcgtg gaatttggcg gtctgtgaac caccgccttt ttagtgcgcc
      481 acttgcatat aggcgtcgcg gacggccgga ccagagccga ccgtctgcga tctgtctatc
      541 agcctcggcg ccaccagccg ccgcgcggct tggccggcgg tgcggtgatt tcagctggat
      601 caggctcggc gggcgccaag gcctgctccg cgaccagctc agcctcgggc tcgggttgag
      661 gctccggtgc tgcgatcggc gcgggcgcgg ggacttccac ctcgatctcg gttggcgcct
      721 cgaccgctac cggttcggcg accacctcgg gggcgacgac ggcttcggcg acggtttcga
      781 tcatggtgtc agcagccgga gtatcgccct tgcgaccgcg cgtgcgggga cggcgggcct
      841 tcttgggggc ttcgtcgctc ggcgcgggca attcgaccca gatgtcactg ggcggagctt
      901 ccgcgataac tgccgggatc ggcggcaact cgacatcgac cggggtgtca accggcgcgg
      961 gacgcgcttc acgctcgcct cggccacgac cgcggctgcg gctcgaacgg ccttccgacc
     1021 ggtccgacct ttcagcgcgc tcaggtcgtt cagcgcgttc cggacgctcg gcttgcggcg
     1081 cgggcgctgc ctgctcaggc gcgggacgac gcgactcgcc gccacgaccg tccagggcgg
     1141 ccgggtcgtg ccaggcaaag gcgctgtcgc cgaatggaac gcgggggcgg gtccagacga
     1201 aggcgtcggc cggacgatcg ccatcctcgc gaccgccacg gcgaccaccg cgacgtccgc
     1261 gacgacgacg gcggcggcgg gcttcctcgt cttcgccttc gagagcgggg aaatcggcgc
     1321 gatcagcgtc gctgtcctcg tcgccgtcgg cgtcttcggc ggcttcgcct tcaggcgcgg
     1381 cgtcggcgtc gtcgcgacga ccgcgacggc gacgcttgcg cttgcggttg ccttcctcct
     1441 cggcggcctc tacgcgcggc tttccacgcg gagcggcgcg agcggctgca gcgtcgtcgt
     1501 cagtatcttc gcgctcaatg gcttcctcgt cgaagatttc ctcgtcttcg tcctcgtcct
     1561 cgtcgtcata gacgtcgtct gcgaaatcat cgtccgacac gggcggcgcc gtgatggcga
     1621 tgcgttcgcc aagctcggtg cgctcgatct cgttttcggc ggcgtgcagg ctctcatcaa
     1681 ccagaacggt cacgaacagg ccgtgggtct gctgcagccg gaccagatag gcgcgctttt
     1741 cgttgaggat gtagatgccg acggcgcgcg agaccttcag ggtaatcgcg ccgctgccct
     1801 tcagggcctc gacctcgaca gcccgcagcg cggccagggc gctggattcg accgagcgaa
     1861 cccgtccggt gccttcgcag tgttcgcaga cgtgagtggt gccttccagc acgccggtgc
     1921 ggcgacgctg gcggctgatt tccatcagac caaaaccgct gatcttgccc atctggatgc
     1981 gggcgcgatc gtccttcagc gcgtccttga ggaccttctc gacggagcga ttgttcttgc
     2041 cctcgtccat gtcgatgaag tcgatgacga tcaggccggc caggtcgcgc agacgcaact
     2101 ggcgcgcggc ctcctcggcg gcttcggtat tggtcttcag cgcggtggct tcgatgttgc
     2161 gttcgcgggt cgccttgccc gagttgacgt cgatggcgac cagggcttca gtctggttga
     2221 tgaccagata gccgcccgac cgcaggggaa cgaccggcga atagatctgc gccagatggt
     2281 cctcgatacg gtgcttcacg aacagcggcg ccggctcgcg atagtggaag accttcttgg
     2341 cctggctggg catcagcatg cgcatgaagt cgcgcgcttc gcggaagccg gcctcgccct
     2401 cgacccagat gccttccagt tccttgtcgt acatgtcgcg gatggcgcgc tttacgaggt
     2461 cttcttcctc atagatcagc gccggggcca cggaatgcag cgtggtctcg cgaatgtttt
     2521 cccaaaggcg cagcagatag tcgtagtcgc gcttgatctc ggccttggtg cgcttggcgc
     2581 cggccgtgcg gatgatcagg cccatgccct gcggc
//
LOCUS       DAOMEO010000387         2615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4229_length_2615_cov_2.403906, whole genome shotgun sequence.
ACCESSION   DAOMEO010000387 DAOMEO010000000
VERSION     DAOMEO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4229_length_2615_cov_2.403906"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1302)
                     /locus_tag="PLH31_09430"
     CDS             complement(115..1302)
                     /locus_tag="PLH31_09430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQR89466.1"
                     /translation="MIPVYVVAAVRTPIGRFRGALAGVRADHLGAHALNELIARAGVG
                     AQHIDDVIFGNVTQIGEQSANIARTSLLGAGWPEAIPAMTVDRKCGSSEAAIHIGVAQ
                     IAAGLSDLVVAGGAESMSRVPMGSNRAIHGEAFGWMVSDRHDPTSQGEAAERIADKYG
                     FDREALDDFAAESHRRAAAATDAGYFRAETVAVPVADLCEKGWEGPRDSLDGDQTIRR
                     DTSREKLSTLKTSFRENGRITAGNASQISDGAAVVLLASEAAVKRFGLTPLALVRSVA
                     VVGSDPTLMLEGPIAASRKAAASAGLSFDDIDLWELNEAFASVPMMWMQATGVGADRL
                     NVNGGAIALGHPLGATGARIATSLIHELARTGQKYGLQAICCAGGLATATVYENLKPA
                     PAD"
     gene            complement(1299..2189)
                     /locus_tag="PLH31_09435"
     CDS             complement(1299..2189)
                     /locus_tag="PLH31_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HQR89467.1"
                     /translation="MAKVAVVGAGLMGVGIAHAFASSGHEVRLIDVSSEQLAKAQKTI
                     AGILADGVRLGKVDEAAAAAALAGLTTSESVTDGAAGADLLVETVSESLSIKIDVVRK
                     AEAVMAPTSLIATNTSALSVTEIAAACQDPTRVIGMHFFNPVQKMKLVELVRGLATTD
                     AAVALCRDYVAQLGKTAIVVNETPGLTTSRMSAMAGNEAMWMLQEGAATAEDIDTALR
                     LGFNHPMGPLELGDLTGWDTRLSVLHYLHSTLGDKFRPCPLIVKMVKAGRYGRKVGWG
                     VYKYENGAKVPGSGLRGSQL"
BASE COUNT          416 a          905 c          842 g          452 t
ORIGIN      
        1 ccggctggcc tcgaacgcca gccccgattc ctccagggcg acatgcgacg ccagagaaca
       61 ggtggtcggc gtataataga aggtcagcct gggcgcggtc atccttcccg ccgatcagtc
      121 cgccggcgcg ggcttcaggt tttcgtacac cgtcgccgtc gccagaccgc ccgcgcaaca
      181 gatcgcctgg agcccgtatt tttgcccggt gcgggccagt tcatggatca ggctggtcgc
      241 gatgcgcgcg ccggtcgcgc ccaagggatg ccccagggcg atggcgccgc cgttgacgtt
      301 cagccggtcc gccccgaccc cggtcgcctg catccacatc atcggcacgc tggcgaaggc
      361 ttcgttgagt tcccacagat cgatgtcatc gaacgacaga ccggcggagg cggcggcctt
      421 gcggctggcg gcgatcgggc cttccagcat cagggtcggg tcggacccga cgacggcgac
      481 cgagcgaacg agcgccagcg gggtaaggcc aaaccgcttg accgccgcct cggaggccag
      541 cagcacgacc gccgcgccgt cggaaatctg tgaggcgttg ccggcggtga tgcgaccgtt
      601 ttcgcggaag ctggtcttca gcgtcgacag tttctcgcgg ctcgtgtcgc ggcggatggt
      661 ctgatcgccg tccagactgt ctcgcggccc ttcccagccc ttttcgcaca gatcggcgac
      721 cgggacggcg acggtttcgg cgcggaaata gcccgcatcg gtcgcggcgg cggcgcggcg
      781 atgggactcg gcggcgaagt cgtccagcgc ctcacggtcg aagccgtatt tgtcggcgat
      841 ccgctcggcg gcctcgcctt gggacgttgg atcatggcga tcggagacca tccagccaaa
      901 cgcctcgcca tggatagccc ggttggaccc catcggcacg cgagacatcg actcggctcc
      961 gccggccacg accaggtcgc tcaggcccgc cgcgatctgg gcgacgccga tatggatcgc
     1021 cgcttcggac gaaccgcatt tgcgatcgac ggtcatggcg gggatcgcct cgggccatcc
     1081 agcgccgagc agcgaggtcc gcgcgatatt ggccgactgc tcaccgatct gggtcacgtt
     1141 gccgaagatc acgtcgtcga tgtgctgggc gcccacgccg gcgcgggcga tcaattcgtt
     1201 cagcgcatgg gcgccgagat gatcggcgcg cacgccggcc agagccccgc gaaaccgccc
     1261 gatcggcgtg cggacggccg cgaccacata gaccgggatc acagctggct cccgcgcagg
     1321 cccgagccgg gcaccttggc gccgttctcg tacttgtaga cgccccagcc gaccttgcgg
     1381 ccatatcgcc ccgccttgac catcttgacg atcagcggac acggcctgaa cttgtcaccc
     1441 agggtagagt gcagataatg cagaaccgaa agccgggtat cccagccggt caggtcgccc
     1501 agttccagcg gccccatcgg gtgattgaaa cccagcctca gggcggtgtc gatatcctcg
     1561 gcggtcgcgg cgccttcctg tagcatccac atcgcctcgt tgccggccat ggccgacatt
     1621 cggctggtgg tcaggccggg tgtctcgttg acgacgatcg ctgtcttgcc cagctgggcg
     1681 acatagtcgc gacagagcgc caccgccgcg tcggtcgtgg ccaggccgcg caccagttcg
     1741 accagtttca tcttctggac cggattgaag aagtgcatcc cgatgacccg ggtcgggtcc
     1801 tggcaggcgg ccgcgatttc ggtcacgctc agcgccgagg tgttggtcgc gatcaggctg
     1861 gtcggggcca tcacggcttc ggccttgcgc accacatcga tcttgatcga gagactttcc
     1921 gagacggtct cgaccagtag atcggcgccg gccgccccgt cggtgacact ctccgatgtc
     1981 gtcagtcccg ccagcgccgc cgccgccgcc gcctcgtcga cctttcccag acggacgccg
     2041 tcggcgagaa tccccgcgat cgtcttttgc gccttggcca gctgttcgga cgaaacgtcg
     2101 atcaggcgca cctcgtggcc cgaggatgcg aacgcatgcg cgatccccac acccatcagg
     2161 ccggcaccca ccactgcaac cttggccaac gcattatcct cttgctgaac cggatcgccg
     2221 cgtcgtcgtg agcgccgatg cgccggcggc tgccggcgtc gccgggccgc gccagacgct
     2281 ggtcctcccc cgtgcgcaac ggcctcaccg tcgtcatcgt tggagaaccc taacgggaaa
     2341 ccggatgtcg cggcaaacaa aaattctctg ttcagaacgt tattctagct tgtaatagat
     2401 tgtggcgctc cgaagagagc caatttcaaa cgtccgggga gcttcgtgaa cttgctcgat
     2461 cagttcggcc cgcctgtcct gccgccaggc ctcgagccgt tgcgcgcgga cgttcgcgcc
     2521 ttcctcgccg actggggctc agcctatccg gcggagaagc gcgctctgtc ctggatgggc
     2581 tttgacgctg atttcagccg cgccctaggc gcgat
//
LOCUS       DAOMEO010000388         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4231_length_2614_cov_2.701055, whole genome shotgun sequence.
ACCESSION   DAOMEO010000388 DAOMEO010000000
VERSION     DAOMEO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4231_length_2614_cov_2.701055"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLH31_09440"
     CDS             <1..931
                     /locus_tag="PLH31_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR89468.1"
                     /translation="LQPSFTPAVMEEWRETYIRPLIQRYFIEPLVPLGKAELVTSVGV
                     MFPIHVVYAVLGFQDDDPAALETFATKALKVLGGMANDPEAKRAAFQAFQELYDPTLA
                     AVQARRTSGAEGADLISRLIRAEFEGRTLSDHQITNFVRMMLPAASETTSRTFAIMMT
                     HLFDHPEVLERLRADRTLMRKVLDESVRHDAVATFKVRECQTDVTLQDVTIPKGSIIS
                     ACVASANRDEAVFDNPDVFDIDRKQMAAFGFGFGAHMCIGMWLAKVEIEEAVGLLLDM
                     LPNLRLDPDHPRPEVRGVSLRGPEAVHVIWDIP"
     gene            complement(1112..>2614)
                     /locus_tag="PLH31_09445"
     CDS             complement(1112..>2614)
                     /locus_tag="PLH31_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89469.1"
                     /translation="NPNFAALPAQVRALYTMPLGNRLEAYGFGLNGSVDLGGATLKSI
                     TGYRFSDTDGVVDTDGTPAPILTTWAGYSSEKWSQELQINGAVGDKFTYIAGAYYSDE
                     KGQEFSRSQNFGFLPYSATTRPNAGFAGQNLADVHNTSTGVFGQGYYQLTDAVRFAGG
                     LRWTWDQRDTTLHNYSAWGVTTTCNLPKPDVAGVCAQTQQAKFDYPAWTFGLDWQVND
                     AVFAYVQTRRASKSGGWNTRAGGLPAFRPETTRDLEAGLKATWLENRLRTNIAVFHSW
                     QSDVQRNAAALTPAGASTQFIVNAGDARVYGIELEGAYRPWTGMELTANLSLMNGEYK
                     SGSFIEQQSVAGAALAGCTPAGASSICPVNRSAEELPQLPKSQYNLGATQALPTSFGK
                     VTLHADYSYLGRQYYNPVTPAAQQSATNKALYAASNAITQTPGYGLLNARIAVEFDAH
                     NIELAVYGKNLTSKEYNVRQFADVYAAGLGFATDFIGEPRTYGASLTKRF"
BASE COUNT          438 a          819 c          873 g          484 t
ORIGIN      
        1 cctgcagccg tcattcaccc cggcggtgat ggaagaatgg cgcgagacct atatccggcc
       61 gctgatccag cgctatttca tcgagccgct ggttccgctg ggcaaggccg aactggtgac
      121 cagcgtcggc gtgatgttcc cgatccatgt cgtctatgcc gtgctgggct tccaggacga
      181 cgatccggcg gcgctggaga ccttcgccac caaggcgctg aaggtcctcg gcgggatggc
      241 caacgacccc gaggccaagc gcgcggcgtt ccaggcgttc caggaacttt acgacccgac
      301 cctggccgcc gttcaggcgc gacggacgtc cggggccgag ggcgccgatc tgatcagccg
      361 cctgatccgc gccgagttcg aaggccggac cctgagcgat catcagatca ccaatttcgt
      421 gcggatgatg ctgccggccg cctccgaaac cacctcgcgc accttcgcga tcatgatgac
      481 ccatctgttc gatcaccccg aggtgctcga acgtctgcgc gccgaccgca ccctgatgcg
      541 caaggtgctc gacgaaagcg tccgtcacga cgcggtcgcc accttcaagg tccgcgaatg
      601 ccagaccgat gtgaccctgc aggatgtgac cattcccaag ggttcgatca tctcggcctg
      661 cgtcgcctcg gccaaccggg atgaagcggt gttcgacaac cccgacgtct tcgatatcga
      721 ccgcaagcag atggcggcct tcggctttgg cttcggcgcg cacatgtgca tcggcatgtg
      781 gctggccaag gtggagatcg aggaagccgt cggcttgttg ctcgacatgt tgcccaacct
      841 gcgactggat cccgatcacc cccgccccga agttcgcggc gtgtcgctgc gagggcctga
      901 ggcggttcac gtgatctggg atatccccta gcagtcgcgc tttggcgatc acgcgggccg
      961 tatcgccaat ctggaaaggc ctccgtcgcc gacggaggcc tttttcttgc ccgctgacca
     1021 tggcgcgcaa tggtcgcaga cgcgctcgat gcaagaaaaa gccgccgacc gggatccggt
     1081 cggcggccag gttcgacggg agtcgccgag gttagaagcg cttggtcaga gaagcgccgt
     1141 aggtccgcgg ctcgccgatg aagtcggtgg cgaagccaag gccggcggca tagacatcgg
     1201 cgaactgacg gacattgtat tctttgctgg tcaggttctt gccgtagacg gcaagctcga
     1261 tgttatgcgc gtcgaattcc acggcgatgc gcgcattcag caagccatag cccggggtct
     1321 gggtgatggc gttcgaggcc gcgtagagag ccttgttcgt ggccgattgc tgggcggccg
     1381 gcgtgacggg attgtagtac tgtcggccga ggtaggaata gtcggcatgc agcgtcacct
     1441 tgccaaacga ggtcggcaga gcctgggtgg cgcccaggtt gtactggctc ttgggaagct
     1501 ggggcagttc ctcggcgctg cggttcaccg gacagatcga cgacgctccc gccggcgtgc
     1561 agcccgcgag cgccgcgccc gccacggact gttgttcgat gaacgagccg ctcttgtact
     1621 caccgttcat caggctgagg ttggccgtca gctccatgcc cgtccagggg cggtaggcgc
     1681 cttccagttc gatcccgtag acccgggcgt cacccgcatt gacgatgaac tgggtgctag
     1741 cgcccgccgg ggtcagtgcc gccgcgttgc gctggacgtc cgactgccag gaatggaaga
     1801 cggcgatgtt ggtccgcagg cggttctcca gccaggtcgc cttcaggccc gcttccaggt
     1861 cgcgggtggt ctcgggccgg aaggccggaa ggccgccggc gcgggtgttc cagccgccgg
     1921 acttggaagc ccgccgcgtc tggacgtaag cgaacaccgc gtcattgacc tgccagtcga
     1981 gcccgaaagt ccaggccgga taatcgaact tggcctgctg tgtctgggcg cagacgcccg
     2041 cgacatctgg tttgggaaga ttgcaggtcg tggtcacacc ccaggctgag tagttgtgca
     2101 acgtggtgtc gcgctggtcc caggtccagc gcagccctcc cgcgaacctg acggcgtcgg
     2161 tcagctggta gtagccctga ccgaacacgc cggtcgaggt gttgtgcaca tcggcgagat
     2221 tctgtccggc gaagccggcg ttggggcgcg tcgtggccga atacggcaag aagccgaagt
     2281 tctgcgagcg gctgaactcc tgccccttct cgtcggaata gtaggccccg gcgatatagg
     2341 tgaacttgtc gccgacggcg ccgttgatct gaagttcctg agaccatttc tcagagctgt
     2401 aaccggccca tgtcgtcagg atcggggcgg gcgtgccgtc ggtgtccacc actccgtcgg
     2461 tgtcgctgaa acgatagccg gtgatcgact tcagtgtcgc gccacccagg tcgaccgagc
     2521 cgttcaggcc aaagccgtag gcttccaacc ggttgcccag gggcatggtg tagagggctc
     2581 tgacttgcgc cggcagggcc gcgaagttcg ggtt
//
LOCUS       DAOMEO010000389         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4233_length_2613_cov_2.779515, whole genome shotgun sequence.
ACCESSION   DAOMEO010000389 DAOMEO010000000
VERSION     DAOMEO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_2613_cov_2.779515"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..691
                     /locus_tag="PLH31_09450"
     CDS             5..691
                     /locus_tag="PLH31_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR89470.1"
                     /translation="MSDPVLSLRGVERVYKTEAGDLPVLRGVDIDIYPGEVVGLIGPS
                     GSGKSSLLHSAGLLERPDAGLVALGGRDCSKLSERVRTRIRLGTVGFVYQFHHLLPEF
                     SALENVAMPGMIAGRSRSEAEARALELLTELGLTARVNHQPAQMSGGEQQRVAIARAL
                     ANKPQLLLADEPTGNLDPTTSAAVFQSLYSLARGQGVAALIATHNMDLARYMDRVFAL
                     KDGHLEPQTF"
     gene            complement(707..1663)
                     /locus_tag="PLH31_09455"
     CDS             complement(707..1663)
                     /locus_tag="PLH31_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HQR89471.1"
                     /translation="MRLIIDTDTAGDDVFSLMLALTRPGVTVEAITIAHGNVGFIQHA
                     ENALLTLEVCARQGVPVYMGAQAPFSRAPLDAAYVFGADGMSDMGYPLAAQRPADGHA
                     VDEIVRRVMAAPGEITLIAQAPLTNIALAYLREPRIARAVKHLWIMGGTDNGVGNVTP
                     AAEYNFYADPEAARIVINAGFNLSIVTWTETLRDGLLDISQLAELEALNTPRARFFTE
                     VNRASLAFARETEGLNGSLHPDALTCAVALDESLILEAEQAVVDVETLGELTRGYLSV
                     SHAILPDIEIADPALKRRPPNARVIKKIDQAGFFAVMRQALL"
     gene            1786..1908
                     /gene="sidA"
                     /locus_tag="PLH31_09460"
     CDS             1786..1908
                     /gene="sidA"
                     /locus_tag="PLH31_09460"
                     /inference="COORDINATES: protein motif:HMM:NF033228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SidA"
                     /protein_id="HQR89472.1"
                     /translation="MEQNKKCVEVPVIRIARESLALVSVASFVWMMCSVAQLVA"
     gene            1933..>2613
                     /locus_tag="PLH31_09465"
     CDS             1933..>2613
                     /locus_tag="PLH31_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQR89473.1"
                     /translation="MSNDEGQGFVHLRVRSAYSLLEGAIKADVIGKLAEAAGMPAAGL
                     TDRANLFGALEYSSYSKDAGTQPIIGCALPVAGIGAGPTERWARAPTITLLAQNERGY
                     LNLSELSSIAYLESSDLAEPIVPWSRVAEHAEGLILLSGGTDGPVDALFAAGKSAEAI
                     QALSEMSRVFGDRFYVELQRHGLPQQAAAEPGLVNWAYEHDVPLVATNDVYFAKAELY
                     DAHDALLCI"
BASE COUNT          457 a          802 c          876 g          478 t
ORIGIN      
        1 cgctatgagt gatcccgtcc tgtcgctccg cggcgtcgaa cgcgtgtaca agaccgaagc
       61 cggtgacctg cctgtgctgc gaggggtcga tatcgacatc tatcccggag aggtcgtcgg
      121 tctcatcggg ccgtcggggt cgggcaagtc atccctgctg cactcggccg gcctgttgga
      181 gcgccccgat gcgggtctcg tggccttggg tggtcgcgac tgctcaaagc tatcggagcg
      241 tgtccgcacg cggatccgtc tgggcaccgt ggggttcgtc tatcagttcc accatctgct
      301 gcccgagttc agcgcgctgg agaacgtggc catgcccggc atgatcgccg gacgcagccg
      361 gagcgaggcc gaggcgcggg ctcttgaact gctgaccgag cttggcctca ccgctcgcgt
      421 gaaccaccag ccggcccaga tgtccggcgg cgaacaacag cgggtggcca tcgccagggc
      481 tcttgccaac aagccccagt tgcttctggc cgatgagccc accggcaatc tagatcctac
      541 gacgtcggcg gcggtgttcc agtcgctcta cagtctggcg cgcggccaag gggtagcggc
      601 gctgatcgcg acccacaaca tggatctggc gcgctacatg gaccgggtct tcgccctgaa
      661 ggatgggcac ctggagccgc agaccttcta agaggtctgc ccggcgctaa agcaaggcct
      721 gacgcataac ggcgaagaag ccggcctgat cgatcttctt gatcacccgg gcgttgggcg
      781 gccggcgctt gagcgcggga tcggcgatct cgatatcggg cagaatggcg tgtgagacgc
      841 tcagatagcc tcgggtcagt tcgcccaggg tctccacatc gaccacggcc tgctcggcct
      901 ccaggatcag actttcgtcc agggcgacgg cgcaggtcag ggcgtccgga tgcagactgc
      961 cgttcagacc ctcggtctcc cgggcaaagg ccagggacgc tctattgacc tcggtgaaga
     1021 accgggcgcg cggggtgttc agggcctcca gctcggccag ttgcgagata tccagcagtc
     1081 cgtctcgaag cgtctcggtc caggtgacga tcgacaggtt gaagcctgca ttgatcacga
     1141 tcctggcggc ctcgggatcg gcatagaaat tgtactcggc cgccggcgtc acattgccga
     1201 ccccattgtc cgtgccgccc atgatccaca gatgctttac ggccctggcg atacgcggtt
     1261 cacgcagata ggccagggcg atgttggtca gcggcgcctg ggcgatgagg gtgatctcgc
     1321 cgggcgcggc catcacccgg cgaacgattt cgtcaaccgc gtgaccgtca gccgggcgct
     1381 gggcggcgag gggatagccc atgtcgctca tgccatcggc gccaaacaca taggctgcat
     1441 cgagcggggc gcgggagaag ggagcctgcg cgcccatata gacgggtacg ccctgacggg
     1501 cgcagacctc cagcgtgagc agcgcgttct ccgcatgctg gatgaacccg acattgccgt
     1561 gggcgattgt gatcgcctcg acagtgaccc ccgggcgggt gagggcgagc atcagggaga
     1621 acacgtcatc cccggcggta tcggtatcga tgatcaagcg catgaggtga tctgacctga
     1681 tccggcgttc agggcaaagg cccggcgggc gctttcgagc gacccgccca aagaaaatgc
     1741 ggtgaatcgt cgcggaacat cttgtgaaca gaacaaaacc ggaatatgga acaaaacaag
     1801 aaatgtgtgg aggttcctgt gatccgtatc gcgcgagagt ctttggcgct tgtgtccgtc
     1861 gccagcttcg tctggatgat gtgctcggtc gctcagttgg tggcctgagt cgattgattg
     1921 gaggcatggg ctatgtcgaa cgacgaggga caagggttcg tccaccttcg ggttcgctcg
     1981 gcctattccc tgctggaagg cgccatcaag gctgacgtca tcggcaagct ggccgaggcg
     2041 gcgggcatgc cggctgctgg tcttacggac cgcgccaatc tgtttggcgc gcttgaatat
     2101 tcctcctact ccaaggacgc agggacccag cccatcatcg gctgcgctct gccggttgcc
     2161 ggtatcggag ccggcccaac cgagcgttgg gcgagggcgc cgacgatcac gctgctggcc
     2221 cagaacgagc ggggttatct caacctctcc gaactgtcgt cgattgccta tcttgagagc
     2281 agcgacctgg ccgagccgat cgtgccctgg tccagggtgg ccgagcatgc ggagggtctg
     2341 atcctgctgt ctggcgggac tgacggtccg gtggacgccc tgttcgccgc cggcaagagc
     2401 gccgaagcga tccaggcgct ctcggaaatg agccgcgtgt ttggcgacag gttctatgtt
     2461 gagctgcaac gccatggcct gccgcagcag gcggcggccg agccgggtct ggtcaactgg
     2521 gcgtacgagc acgatgtgcc gctggtcgcg accaacgatg tgtatttcgc caaggccgaa
     2581 ctctacgacg ctcacgacgc cctgctgtgc atc
//
LOCUS       DAOMEO010000390         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4236_length_2612_cov_2.457567, whole genome shotgun sequence.
ACCESSION   DAOMEO010000390 DAOMEO010000000
VERSION     DAOMEO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4236_length_2612_cov_2.457567"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLH31_09470"
     CDS             <1..487
                     /locus_tag="PLH31_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQR89474.1"
                     /translation="PVVVREMHDELVIDFHLSRGNVLVPHLVQGFRAVMLATGPDAYI
                     SPDGYESADQVPTWNYLSVEAEGSVTVLDEAELIAQLDSLSEQEEARLLPKPLWTRHK
                     MAPGKFEAMLRGIIGGRLLVDRLEGTFKLSQNKSEADRLEAAKGLGEHPIAAMMRVKP
                     E"
     gene            complement(474..860)
                     /locus_tag="PLH31_09475"
     CDS             complement(474..860)
                     /locus_tag="PLH31_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR89475.1"
                     /translation="MILLLDTQLLIWATAFSPTLSKRARALLTDPDNQLVFSVASIWE
                     ASIKQARGRPDFNIDTHLLRRALLSNGWEELPVTSEHALATMNLPPLHKDPFDRLLLA
                     QAISEGMTLLTSDSTLAGYPGPIIQA"
     gene            complement(857..1102)
                     /locus_tag="PLH31_09480"
     CDS             complement(857..1102)
                     /locus_tag="PLH31_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQR89476.1"
                     /translation="MRTVNIHEAKTHLSRLVEQAADGESFIIAKAGKPMVKVVPLEVR
                     EQPAVRRIGFLDGEFTVPDDFDTMFADEILAMFEGED"
     gene            1199..1593
                     /gene="rnpB"
                     /locus_tag="PLH31_09485"
     ncRNA           1199..1593
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLH31_09485"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1885..2352
                     /locus_tag="PLH31_09490"
     CDS             1885..2352
                     /locus_tag="PLH31_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQR89477.1"
                     /translation="MFLSTFEKQVDSKRRIVVPQDFRAAISGPFDGVVCFPSIEFDCL
                     EGGGKALYDRYLGVIEELEFGDPVRTALETSVLGGMARLSFDTAGRITLPDHLCEMFG
                     LSDWVTVVGMGERFQIWSREAFQAHRAAQRDLAREGLSAMRVQQRTARLGGAA"
     gene            2349..>2612
                     /gene="mraW"
                     /locus_tag="PLH31_09495"
     CDS             2349..>2612
                     /gene="mraW"
                     /locus_tag="PLH31_09495"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HQR89478.1"
                     /translation="MTEAAPHISVLLNEIVDALAPKPGETIIDGTFGAGGYTRAILAT
                     GASVVGFDRDPTVLRFTEGLPTDRFRLIQDRFSRMGEHFEPQSV"
BASE COUNT          467 a          787 c          857 g          501 t
ORIGIN      
        1 gccggtcgtg gtgcgcgaga tgcacgatga actggtcatc gacttccacc tgtcgcgcgg
       61 caacgtgctg gtcccgcacc tcgtccaggg cttccgcgcc gtgatgctgg cgacggggcc
      121 ggacgcctac atcagccccg acgggtacga gagcgctgac caggtgccga cctggaacta
      181 cctctccgtc gaggccgagg ggtcggtgac tgtcctggat gaagccgaac tgatcgccca
      241 gctcgacagc ctgtctgagc aggaagaagc gcgcctcctg cccaagccgc tctggacccg
      301 ccacaagatg gcccccggca agttcgaggc gatgctgcgc ggcatcatag gcggaaggtt
      361 gttggtcgac cgcctggagg ggacgttcaa gctgtcgcag aacaagagtg aggcggatcg
      421 tctagaggcg gcgaaggggc tgggggagca tccgatcgcc gcgatgatgc gcgtcaagcc
      481 tgaatgattg ggccggggta gccggccaaa gtcgagtctg acgtcagcaa cgtcatgcct
      541 tccgagatcg cttgcgccaa caacagccga tcaaatgggt ctttatgcaa gggcggtagg
      601 ttcatcgtcg ccagggcatg ctcgctggtg acgggaagct cttcccagcc gttgctcagc
      661 aaggcgcgac gaagcagatg ggtgtctatg ttgaagtcgg gacgtccgcg cgcctgtttg
      721 atgctggctt cccaaatgct cgcgacgctg aagaccaact gattatctgg atcggtcagc
      781 agagcgcgcg cgcgcttcga cagtgtcggg gagaatgcgg tggcccagat cagcaattgg
      841 gtatcgagga ggaggatcaa tcctcgccct cgaacatcgc caggatttcg tcggcgaaca
      901 tcgtatcgaa atcgtcaggg acggtgaact cgccgtcaag aaagccgatg cgacgcacgg
      961 ctggctgttc gcgaacttca agcggtacca ccttgaccat cggcttgccg gcctttgcga
     1021 tgataaagct ctcgccatcc gccgcctgtt cgaccaagcg cgacaggtgg gtcttggctt
     1081 cgtggatatt gacggtgcgc atgatgcgcc tccatgaact aagtccgctt agtttagtct
     1141 atttcctgcc gatctacaat tgacctccgc cccatccccc gtcaaacacc gtcccggacc
     1201 agacggtcgg gcggtcgcgg tcttcgcaag aaggtcgagg aaagtccggg ctccacggtg
     1261 acaaggcggc gggtaacgcc cgccgggagc gatcccaggg atagcgccac agaaagcaaa
     1321 cctcctccct ttcgagggcg ggaagggtga aagggtgggg taagagccca ccgcggacct
     1381 ggcgacaggg acggcatggc aagccccgcc tggagcaaga ccgaataggg accccgcgcc
     1441 gacctcgcgt cggataggcc gtcactggct gagaagggtc cgggtaggtc gcgagaaccg
     1501 tccagcaatg ggcggtccag aggaatgatc gtcgcgctct agcctttcgg ggcaagggtg
     1561 gcacagaacc cggcttatag accgtctggt ccgtctctcc ccgtcttgat tgtggcgggg
     1621 aggtggcggg cgtccccaat cccccgttgt cggacgattt ggccccttgc gggacaagcc
     1681 tttgcgcgcc cctaacactt aacagttctg tgtatgttct gttgatggct tgtgatcaca
     1741 agtcacgaac cagtgcgaca tctttgtttt cacggcaaaa tgggcgctgg tgtcattgtt
     1801 tcccaattca tcccatgtta acccagttgg gtcggcggcg atcgaatttc gccgcgcggc
     1861 cggggtctca aggggtaggg cgaggtgttc ctctcgacgt tcgagaagca ggtggacagc
     1921 aagcggcgca tcgttgtgcc gcaggacttc cgcgcggcga tctcgggccc cttcgatggc
     1981 gtggtctgct ttccctccat cgagttcgat tgcctcgagg gtggcggaaa agccttgtac
     2041 gaccgttact tgggcgtgat cgaggagctg gagttcggcg atccggtccg caccgcgctt
     2101 gaaaccagcg tcctgggcgg catggccagg ctgtcctttg atacagctgg tcgcattacg
     2161 ctgcccgatc acctgtgcga gatgtttggt ctcagtgatt gggtgaccgt cgtcggtatg
     2221 ggtgagcgtt ttcagatctg gtcgcgcgag gccttccagg cccaccgcgc cgcgcagcgt
     2281 gacctggccc gcgaaggtct gtccgccatg cgggttcagc agcgcacggc gcggctgggt
     2341 ggcgcggcat gaccgaggct gccccccaca tttccgtcct gctcaacgag atcgttgacg
     2401 ccctggcgcc caagccgggc gagacgatca tcgacggcac cttcggggcg ggtggctata
     2461 cccgcgccat cctggccacc ggcgccagcg tggtgggctt cgaccgcgac ccgaccgttc
     2521 tgcggttcac cgaaggcctg ccgaccgacc gctttcgcct gatccaggac cgcttctcgc
     2581 ggatgggcga gcatttcgag ccgcagtcgg tc
//
LOCUS       DAOMEO010000391         2610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4241_length_2610_cov_2.539335, whole genome shotgun sequence.
ACCESSION   DAOMEO010000391 DAOMEO010000000
VERSION     DAOMEO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4241_length_2610_cov_2.539335"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLH31_09500"
     CDS             complement(<1..214)
                     /locus_tag="PLH31_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89479.1"
                     /translation="MPPVKKTKSSGGLFWDLLTFDRLVTGPVIHLIYWAGLGVVALFG
                     FSVVGAAVGLALKEPGVGSLLLAFPVL"
     gene            complement(323..814)
                     /locus_tag="PLH31_09505"
     CDS             complement(323..814)
                     /locus_tag="PLH31_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89480.1"
                     /translation="MNRTRIALFAAAATLTFAGAAFAQTALTPGQQIPMTASPMATPT
                     APMTTPVPPAEATTTTSSPVTATTTPPAAPNADTGAAPASAVVASDFKVGSEVRDPAG
                     VTIGTISGTSAAADGSVNVVIANGATKFALPSASLSSKAGVLATTATKAQIDATLAAA
                     KPQ"
     gene            complement(931..>1863)
                     /locus_tag="PLH31_09510"
     CDS             complement(931..>1863)
                     /locus_tag="PLH31_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HQR89481.1"
                     /translation="DQIRALRKTLATEFGFVMPPVRILDNMRLANQGYAIRIKEMEAG
                     AGEVRLGMLMAMDPRGGQVELPGEHVREPAFGLPATWVADDLREEATFRGYTIVDPAT
                     VLTTHLTEILKENMADLLSYSEVQKLLKDLPEGQKKLVDDLIPGVATATTVQRVLQAL
                     LKERVSIRDLPQILEGIGEAAPHTSSVTQLVEQVRARLARQLCWANRGDDGALPIITL
                     SAEWEQAFAEALVGPGDDKQLALAPSRLQEFIRGVREAFERAALQGEAAVLLTSPGVR
                     PYVRSIIERFRGQTVVMSQNEIHPRARLKTVGMV"
     assembly_gap    1864..1873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1874..>2610)
                     /locus_tag="PLH31_09515"
     CDS             complement(<1874..>2610)
                     /locus_tag="PLH31_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HQR89482.1"
                     /translation="VTKGSGRIAEVAARFTLDAIPGKQMAIDADLSTGLIDQDTAKQR
                     RKDLEQESTFFGAMDGASKFVKGDAVAGLIITGINIIGGILIGVIQHKIPIGEASATY
                     TLMTIGDGLVSQIPALIISIAAGMVVSKAGVEGSANAALTKQLSMNPVALGMVSASSG
                     VMALIPGMPILPFAALSLGAGFLAYRRSMTAKLPQPIDPELAEAAASEPEEEPISASL
                     AIDDVKIELGYGLLTLINDLDGRRLTD"
BASE COUNT          422 a          835 c          924 g          419 t
ORIGIN      
        1 tcaacacggg gaaggccagc aacagcgagc ccacaccggg ctccttcaac gcaaggccca
       61 cggccgcgcc aacgacggaa aagccgaaca gggccacgac gcccaggccg gcccaataga
      121 tcaggtggat caccggaccc gtcacgagcc ggtcaaaagt caggaggtcc cagaacaggc
      181 ctccgctgga ttttgtcttc ttgaccggag gcatacgaac aaacccgaaa tcggcgaaaa
      241 cccgccgcct tggcgcatgg ctatcagcgc aaagcccgct cggcaatgcc gggcgggcca
      301 gagcttctgc gagcggaagt ccttattggg gcttggcggc cgccagggta gcgtcgatct
      361 gggccttggt ggcggtggtg gcgaggacgc cggccttgga agacaggctg gcgctgggca
      421 gggcgaattt cgtcgcgcca ttggcaatca cgacattgac cgaaccatcg gctgcggcgc
      481 tggtcccgga gatcgtgccg atggtcacgc cggccggatc cctgacttcc gagccgacct
      541 tgaagtcgct ggcgaccacc gcagaggccg gcgcggcgcc ggtgtcggca ttgggcgcgg
      601 ccggcggggt ggtcgtcgcg gtcaccggcg aggaggtcgt cgtggtcgcc tcggcaggcg
      661 ggactggcgt cgtcatgggc gcggtcgggg tcgccatggg cgaggcggtc atggggatct
      721 gttgaccggg ggtcagagcg gtttgcgcga aggccgcccc ggcgaaagtc agggtcgcgg
      781 cggcggcgaa aagggcgatt ctggtgcggt tcatctgaaa actccgtcgg tgtcgtcagc
      841 ccctacccgg ggaaactaac gtcgccgctc gggggcggtt ccggcggcga ctgacacttc
      901 aggttgttgc aacggcctga cggcgcgccg tcagaccata ccgacagtct tgagtcgggc
      961 gcgcggatgg atctcgttct ggctcatcac caccgtctgg ccccggaagc gctcgatgat
     1021 cgaccggaca taggggcgca cgccggggct ggtcagcagg accgcggcct cgccctgcag
     1081 cgcggcgcgt tcgaaggcct cgcggacccc gcggataaac tcctgcaggc gcgacggcgc
     1141 cagggccagc tgcttgtcgt cgcccggacc gaccagagcc tcggcgaagg cctgctccca
     1201 ctcggccgac agggtgatga tcggcagggc gccgtcgtcg ccgcgattgg cccagcacag
     1261 ctggcgggcc agacgggcgc ggacctgctc gacaagctgg gtgaccgagc tggtgtgcgg
     1321 ggcggcctcg ccgatgcctt ccaggatctg cggcaggtcg cggatcgaca cccgctcctt
     1381 gagcagggcc tgcagcacgc gctgtacggt ggtggcggtg gcgacgccgg ggatcagatc
     1441 gtcgaccagc ttcttctggc cctcgggcag gtccttcagc agcttctgca cttccgaata
     1501 ggacagcagg tcggccatgt tttccttcag gatctcggtc aggtgcgtgg tcagcacggt
     1561 ggccggatcg acgatggtgt agccgcggaa ggtggcttcc tcgcgcaggt catcggccac
     1621 ccaggtggcc gggaggccaa aggctggctc gcgcacatgc tcgccgggca gttcgacctg
     1681 gccgccgcgc ggatccatgg ccatcagcat gcccaggcga acctcgccag cgccggcttc
     1741 catttccttg atgcggatcg catagccctg attggccagg cgcatgttgt ccaggatgcg
     1801 caccggcggc atgacaaagc cgaactcggt ggccagggtc ttgcgcaggg cgcggatctg
     1861 gtcnnnnnnn nnnggtcggt aaggcggcgg ccgtccagat cgttgatcag ggtcagcagg
     1921 ccatagccca gctcgatctt gacgtcgtcg atcgccaggg acgcgctgat cggctcttct
     1981 tccggctcgc tggcggccgc ctcggccagc tcgggatcga tcggctgggg cagcttggcg
     2041 gtcatcgagc ggcgataggc caggaagccc gcccccagcg acagggccgc gaacggcagg
     2101 atcggcatcc ccgggatcag ggccatgacg cccgacgagg ccgagaccat gcccagcgcc
     2161 accgggttca tcgacagctg cttggtcagg gcggcgttgg ccgagccctc gacgccagcc
     2221 ttcgacacga ccatgccggc cgcgatcgag atgatcaggg ccggaatctg gctgaccagg
     2281 ccatcgccga tggtcatcag cgtataggtg gccgaggcct cgccgatcgg gatcttgtgc
     2341 tggatcaccc cgatcaggat gccgccaatg atgttgatcc cggtgatgat caggccggcg
     2401 acggcgtcgc ccttcacgaa cttcgatgcg ccgtccatgg cgccgaaaaa ggtgctttcc
     2461 tgctccagat ccttgcggcg ctgcttggcc gtgtcctggt cgatcaggcc ggtcgacagg
     2521 tcagcgtcga tggccatctg cttgccggga atggcatcga gggtgaagcg ggcggcgact
     2581 tcggcgatcc ggcccgaacc cttggtcacg
//
LOCUS       DAOMEO010000392         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4248_length_2604_cov_2.663005, whole genome shotgun sequence.
ACCESSION   DAOMEO010000392 DAOMEO010000000
VERSION     DAOMEO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4248_length_2604_cov_2.663005"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLH31_09520"
     CDS             <1..547
                     /locus_tag="PLH31_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQR89483.1"
                     /translation="ALHKSVRGSDPDAALYWLARMLEGGEDPLFIARRLVRMASEDIG
                     AADPLSLLLTTAAKDAFDFLGSPEGELALAQCVVHMASAPKSNAVFTAFKAAKRAAKE
                     TGSLAPPAHILNAPTKLMKSLGYGDGYAYDHDVEGGVSGQNYFPDGMARARFYEPKPI
                     GAEAKVRERLDAWRKLRRDGK"
     gene            615..842
                     /locus_tag="PLH31_09525"
     CDS             615..842
                     /locus_tag="PLH31_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89484.1"
                     /translation="MAKSVDWEKREADLKVRLKLFVTNATTYAYAVMGASFIEPYIKG
                     AGFKAASYVGIGLALVLHAIAWYAAPHGEKP"
     gene            839..973
                     /locus_tag="PLH31_09530"
     CDS             839..973
                     /locus_tag="PLH31_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89485.1"
                     /translation="MTPLEAMIYILGPILIAVGIYALFLKRELDELQAQRHRETHSAE
                     "
     gene            1074..1778
                     /locus_tag="PLH31_09535"
     CDS             1074..1778
                     /locus_tag="PLH31_09535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQR89486.1"
                     /translation="MARSWVIHEDDSVIVLNKPAGLSSQGGRIQAHTLDDLLWAFARP
                     GRPRPRLIHRLDRDTSGVILTAKTKPGAAFLGKALMGKRFRKSYMAIVAPGAPEPKGG
                     MIDSPLRRESIGREAYMRVCPPDHADAETARSRYRTLSSNATAALVELSPDTGRMHQL
                     RVHMASIGRPISGDSRYGGALMLGGTAVPRLMLHAAQLVFPHPEGGERRIAASIPADM
                     ATMLEKLGLPLPEQRA"
     gene            1811..2308
                     /locus_tag="PLH31_09540"
     CDS             1811..2308
                     /locus_tag="PLH31_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89487.1"
                     /translation="MKRLAGLVLSLALLGGCASAPTLYAARATPAGVGYTEFRLEPGR
                     YRVTFQGGPGAPVEQVSDYALLRAAELAVRDGYDWFRVSDRVTSSSRAGNGPRLSLGG
                     GSSRYGGRSAVGLGVGTSFNLGPGPAVTSSLEVVFGKGPAPRDADVYVAQAIIRSVGY
                     GRSQT"
     gene            complement(2309..>2604)
                     /locus_tag="PLH31_09545"
     CDS             complement(2309..>2604)
                     /locus_tag="PLH31_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89488.1"
                     /translation="LTRGVIARVPPEKDALLGLKDARLSGLTLDASDLAGPAERIATD
                     IMNFGRDTRGVAPMTILQGLPAEGYFAAAETAGLTHAGLRATHPLAASRATAA"
BASE COUNT          400 a          899 c          873 g          432 t
ORIGIN      
        1 ggccctgcat aagtcggtgc gcggcagcga tccggacgcg gcgctctact ggctggcgcg
       61 gatgctggag ggcggcgagg acccgctttt catcgcccgc cggctggtgc gcatggcgtc
      121 cgaggacatc ggcgcggccg atccgctctc gctccttctg accaccgccg ccaaggacgc
      181 cttcgacttt ttgggatcgc ccgagggcga actggccctg gcccagtgcg tggtccacat
      241 ggccagcgcg cccaagtcga acgcggtctt cacagccttc aaagccgcaa agcgcgccgc
      301 caaggaaacc ggctccctgg ccccgcccgc ccacatcctc aacgcgccca cgaagctgat
      361 gaagtcgctc ggctatggcg acggctatgc ctatgaccac gatgtcgagg gcggcgtctc
      421 gggccagaac tacttcccgg acggcatggc gcgggcccgg ttctatgagc ccaagccgat
      481 tggcgccgag gccaaggtgc gcgagcggct ggacgcctgg cgcaagctgc ggcgggacgg
      541 gaagtaggcg agttaaagct tgcgctcacc cgaagattgt tctttatttg ttctcgttgg
      601 attgcgagag cacgatggcg aagtcggtcg attgggaaaa gcgcgaggct gacctgaaag
      661 tgcggctcaa gctcttcgtc accaatgcga ccacctatgc ctatgcggtc atgggcgcat
      721 cgttcatcga gccctatatc aaaggcgcgg gtttcaaggc ggcgagctat gtaggcatcg
      781 ggctcgcgct cgtgctgcac gcgatagcgt ggtacgctgc ccctcacgga gagaagccat
      841 gacgccgctt gaggccatga tctacatcct cgggccgatc ttgatagcgg tcggcatcta
      901 tgcgcttttc ctcaagcgcg agcttgatga gttgcaagcc cagcgtcacc gcgagactca
      961 ctccgctgaa taagcctttt ccctcggacc aacgtccccc ccgccgtccg cccctcgcgc
     1021 cgaaacccaa ggcctccgac gctcccatca ccctgaccga ggacgagatc gccttggcgc
     1081 ggtcgtgggt gatccatgag gacgacagcg tcatcgtgct gaacaagccg gccggcctgt
     1141 ccagtcaggg cggccgcatc caggcccata cgctggacga cctgctgtgg gccttcgcgc
     1201 gtcccggccg gccgcgtccg cgcctgatcc accggctgga ccgcgacacc tcgggcgtca
     1261 tcctgaccgc caagaccaag ccgggggcgg cgttcctggg caaggccctg atgggcaagc
     1321 gctttcgcaa gtcctacatg gccatcgtcg cgcccggcgc gccggagcct aaaggcggga
     1381 tgatcgacag tccgctacga cgcgagtcga tcggccgcga ggcctatatg cgcgtctgcc
     1441 cgcccgatca cgccgacgcc gagaccgctc gcagccgcta tcgtaccctg tcgtccaacg
     1501 ccaccgccgc cctggtcgaa ctgtcgccgg acaccggccg catgcaccag ttgcgcgttc
     1561 acatggcctc gatcggccgg ccgatctcgg gcgacagccg ctatggcgga gcgcttatgc
     1621 tgggcgggac ggcggttccg cgcctgatgc tgcacgccgc ccagctggtg tttccgcatc
     1681 ccgaaggcgg cgagcgccgg atcgccgcct cgatccccgc cgacatggcg accatgctgg
     1741 agaagcttgg attgccgctc ccggaacagc gcgcctagcc aagccgttta gccagcagga
     1801 gaaaaacgcc atgaaacgcc ttgccggtct ggtcctgtcc cttgcgctgc ttggcggctg
     1861 cgccagcgcg cccaccctct atgccgctag ggcgacgccg gcgggtgtgg gctataccga
     1921 gttccggctc gagcctggcc gctatcgcgt cacgtttcag ggcggccccg gcgcgccggt
     1981 cgagcaggtg tcagactatg cgctgctgcg cgccgctgaa ctggcggtgc gcgacggtta
     2041 tgactggttc cgggtgtctg accgggtgac gtccagctcc cgcgccggca atggcccgcg
     2101 cctgtcgctc ggcggcggct ccagccgcta tggcggccgc agcgcggtgg ggctgggtgt
     2161 cgggaccagc ttcaaccttg gcccgggccc ggctgtgacc agctcgctgg aggtggtgtt
     2221 cggcaagggg cccgcgccac gcgacgccga cgtctatgtg gcccaggcga tcatccgttc
     2281 cgtcggctac ggccgtagcc agacctgatc aggcggctgt cgcccggctg gccgccagcg
     2341 gatgggtggc gcgcaggccg gcatgggtga ggccggccgt ttcggccgct gcaaaatagc
     2401 cctcggcggg caggccctgc agaatggtca tgggcgcaac gcccctggtg tcgcggccga
     2461 agttcatgat gtcggtcgcg atccgctcgg cggggccggc gaggtccgag gcgtccagcg
     2521 tcagaccgct caggcgcgcg tccttcagcc cgagcaaggc gtccttctcc ggcggtacac
     2581 gggcgatgac gccgcgcgtc aggg
//
LOCUS       DAOMEO010000393         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4249_length_2604_cov_2.266771, whole genome shotgun sequence.
ACCESSION   DAOMEO010000393 DAOMEO010000000
VERSION     DAOMEO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_2604_cov_2.266771"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..625
                     /locus_tag="PLH31_09550"
     CDS             17..625
                     /locus_tag="PLH31_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89489.1"
                     /translation="MSKFDQFKAKLRPGQTYRRADLAQWSTAVDRHLKEAVQAGVLTK
                     LAGGLYYAPKQTVFGKAPPEDDTLVSTFLKGGPFLLASPNAYNALGVGTTQLHNKTVV
                     YNHKRHGKFALGGRTYDFRMKPAFPKKLTAEFLMVDLVNNLDQLGESAEDVLERVKAR
                     LAASDRARVKKAAQSYGSERAKKFFARALAEVGAEVGAQDAA"
     gene            615..1562
                     /locus_tag="PLH31_09555"
     CDS             615..1562
                     /locus_tag="PLH31_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQR89490.1"
                     /translation="MPRDFLHNHREFADLIRIVAEAQGIAPALGEKDYWIMQSLYGLQ
                     QLGLTFELKGGTSLSKGYGLISRFSEDIDIRIAPPADPPVMTGRNHDKPAHVQSRRDF
                     YDRLAQTIVIDGITSVERDTAFDDPRQYRSAGIRLTYASINGSVEGLKDGVLLEVGFD
                     DVAPNEPRDISSWAYDFAASRVEILDNRALSVACYHPGHTLVEKLQAISTKFRQQQET
                     GAFPPNFMRHYYDVYSLLQDPTVQAFIGTQGYLDHKDKRFPKADNPVIAENEAFVLSD
                     PETRATLQKAYIASSALYFRGQPAFDEILAEIAKWAPKL"
     gene            complement(1584..>2338)
                     /locus_tag="PLH31_09560"
     CDS             complement(1584..>2338)
                     /locus_tag="PLH31_09560"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89491.1"
                     /translation="ACVVFLARQARRAETVRRLAELRVALADVADVHPHHLAWDKIAI
                     DRSNRRWRSLLALARLFLRRDWQATHRANDLRDLAGVTLLFPMNDLFEAYVAALLRKA
                     LAPEGYEVVSQGGLRYCLNEIAPDGTLGRPLFRTKPDCMVRRNGRTVLVIDIKWKRLS
                     AAGSDPKRGVSQADVYQLMAYARLYDSARLMLLYPHHPDLEGEGALARHRLNVAGADE
                     RLTIASIDVAADRLATMASLKALVLGEAMVPA"
     assembly_gap    2339..2348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2349..2574)
                     /locus_tag="PLH31_09565"
     CDS             complement(<2349..2574)
                     /locus_tag="PLH31_09565"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89492.1"
                     /translation="MEILPKIDQLGDAVSSEGRAGLRHRLVQMLDVALGLEINLGRDA
                     AMARQDETLLDILIRAFADQLTNEVRRGLPR"
BASE COUNT          498 a          876 c          810 g          410 t
ORIGIN      
        1 ggcctaagtt gaagccatga gcaagttcga ccagttcaag gccaagctcc gcccggggca
       61 gacgtatcgc cgggcggacc tggcccaatg gtcgacggcg gtcgatcgtc acctcaagga
      121 agcggtgcag gccggcgtgc tgaccaagct ggccggcggc ctctactatg cgcccaaaca
      181 gacggtgttc gggaaggccc cgcctgagga cgacaccctg gtgtcgacct tcctcaaggg
      241 cggtccgttc ctgctggcct cgcccaacgc ctacaatgct cttggggtcg gcacgaccca
      301 gctgcacaac aagaccgtgg tctacaacca caagcgccac gggaaattcg cgctcggcgg
      361 ccgcacctac gattttcgga tgaagcccgc gttcccgaag aagctcacgg ctgagtttct
      421 gatggtcgac ctggtcaaca acctcgatca gctcggcgaa agcgccgaag acgtgctcga
      481 gcgcgtcaag gcgcggctgg ccgccagtga ccgggcgcgc gtgaaaaagg ccgcccagtc
      541 ctacggctcc gagcgggcca agaagttctt cgcccgcgcg ctggctgagg ttggggccga
      601 ggtcggagcc caggatgccg cgtgatttcc tgcacaacca tcgcgagttc gccgatctga
      661 tccggatcgt cgctgaggcg cagggcatcg cgccggccct gggcgagaag gactactgga
      721 tcatgcagag cctctacggc ctgcagcagc tgggcctgac cttcgagctg aagggcggca
      781 cctcgctctc caagggctac ggactgatca gccgtttctc ggaagacatc gacatccgca
      841 tcgcgccgcc cgccgatccg ccggtgatga ccggccgcaa ccacgacaag ccggcccacg
      901 tccaaagccg cagggacttc tatgatcggc tcgcgcagac catcgtcatc gacggcatca
      961 cctcggtcga gcgcgacacg gcgttcgatg accctcgcca atatcgcagc gccggcattc
     1021 gcctaaccta cgccagtatc aatggatcgg tggaaggcct gaaagacggc gtgctgctgg
     1081 aagtcggctt cgacgatgtc gcgcccaacg aaccgcgcga catcagctcg tgggcctatg
     1141 acttcgccgc cagccgggtc gagattctcg acaaccgcgc cctgagcgtc gcctgctacc
     1201 accccggcca caccctcgtg gaaaagctgc aggcgatctc caccaagttc cgccagcagc
     1261 aggagaccgg cgcgttcccg cccaacttca tgcgccacta ctacgacgtc tactccctgc
     1321 tccaggatcc gaccgtgcag gcgtttatcg gcacgcaagg gtatctcgac cacaaggaca
     1381 agcggttccc gaaggccgac aatccggtca tcgccgagaa cgaggccttc gtactcagcg
     1441 acccagagac ccgggccacg ctgcagaagg cctacatcgc ctcaagcgcg ctctatttcc
     1501 gcggccaacc agccttcgac gagatcctgg ccgaaatcgc taagtgggcg cccaagctgt
     1561 aagtcagtag cctcccgcca ccatcaggcc gggaccatcg cttcaccgag gaccaaggct
     1621 ttcagcgacg ccatggtcgc cagacggtcc gccgcgacgt cgatgctggc gatcgtcagc
     1681 cgttcatcgg cgccggcgac attgaggcgg tgacgtgcga gcgcgccctc tccttcaagg
     1741 tccggatgat gcggataaag gagcataagg cgcgccgagt catagagccg ggcataggcc
     1801 atgagctggt agacatcagc ttgggagacg ccacgctttg ggtcactgcc ggcggcgctc
     1861 aaacgcttcc atttgatgtc gatcaccaac acggtgcggc cgttccggcg caccatgcag
     1921 tcgggcttgg tgcggaacaa gggtcgcccc agcgtgccgt cgggcgcaat ctcattgagg
     1981 caatagcgca gcccaccttg cgagacgacc tcgtagccct ctggggccag ggccttgcgc
     2041 agcagcgccg cgacataggc ctcgaagagg tcgttcatcg gaaacaacaa ggtgacgccg
     2101 gccaagtcgc gcagatcgtt ggccctatgg gtcgcctgcc agtcgcggcg aagaaacagc
     2161 cgcgccaggg ccagcagcga gcgccaacgg cggttcgatc ggtcgattgc gatcttgtcc
     2221 caggccaaat gatgcgggtg gacgtcggcg acatcggcca gggcgacgcg gagctctgcg
     2281 agcctgcgca cggtttcggc gcggcgggcc tgtctggcaa ggaataccac gcacgcctnn
     2341 nnnnnnnngg cgcggtagcc cgcgtctcac ctcattggtc agctggtcgg cgaaggcgcg
     2401 gatcaggatg tccagcaggg tttcatcttg ccgcgccatg gcggcgtcac ggccaagatt
     2461 gatctccagg cccagggcca cgtccaacat ctgcaccagg cgatggcgca gcccggcgcg
     2521 gccctccgag gacacggcgt cgccgagctg gtcgatcttc ggcaggatct ccaagctaca
     2581 gccttcgccc gcgacgatgc cgac
//
LOCUS       DAOMEO010000394         2595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4266_length_2595_cov_3.093307, whole genome shotgun sequence.
ACCESSION   DAOMEO010000394 DAOMEO010000000
VERSION     DAOMEO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4266_length_2595_cov_3.093307"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..322)
                     /locus_tag="PLH31_09570"
                     /pseudo
     CDS             complement(1..322)
                     /locus_tag="PLH31_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516647.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     assembly_gap    323..371
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<372..1271)
                     /locus_tag="PLH31_09575"
     CDS             complement(<372..1271)
                     /locus_tag="PLH31_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR89493.1"
                     /translation="MTQSLPSPAAHARAVLADLGVAGLPADGGVPVRCPIDGSILTQA
                     AYDDARAIDAKVSAAAKAFREWRTVPAPRRGELVRLLGEELRAAKDQLAAIVTLEAGK
                     IASEAAGEVQEMIDICDFAVGLSRQLYGLTIASERPGHAMRETWHPLGPVLVISAFNF
                     PVAVWAWNAALALVCGNPVIWKPSEKTPLTAIATHTILERALARFGDAPEGLCSVVLG
                     GRDAGERLVRDPAIPLVSATGSTRMGQAVAPIVAGRFGRSILELGGNNAMIVTPSADL
                     SLAVRAIVFSAAGTAGQRCTSLRR"
     gene            1389..2282
                     /locus_tag="PLH31_09580"
     CDS             1389..2282
                     /locus_tag="PLH31_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQR89494.1"
                     /translation="MDDNAAYIQRLEALRAGVDRRLAELAPRTGTAPDRLVEAVRYAL
                     LAPGKRFRPMLTLLTAAQFGAGEGAALDTACAFEMVHASSLILDDLPSMDDAGLRRGL
                     PTIHRAFDEATAVLAGVGLLNQAYAVVAADTRLESAMRAEIAGRIADSVGFCGLIAGQ
                     ARDLFDRDQLRDLPALDRLNHEKTGVLIMAAAQSGARIAGASDDAVAAVGEFGRRMGL
                     AFQIRDDLIDAEGSVDGAGKDVGKDEGMTTAVSLLGVAGARAAMEAHLAAGRAALESV
                     GGCDLVAGYVGRLFAGRKAAA"
     gene            2279..>2595
                     /locus_tag="PLH31_09585"
     CDS             2279..>2595
                     /locus_tag="PLH31_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89495.1"
                     /translation="MTASPILTVTGVAHAYGDKAVLHGVDLALSPGEIYALLGPNGAG
                     KTTLIKTICGRFRPDRGEVRLNGQDPARVNAARGSLGLVPQEIALYAHLTVAENLAAF
                     ASLA"
BASE COUNT          368 a          884 c          938 g          356 t
ORIGIN      
        1 cacatcgttc tggatcgcca cggcctcgtc gaagtcgcga tagggcacga cgtgcagcag
       61 cggcgcgaag gtctcgcgtt gcatccccgc gctgggggcg ggcaggcggg ccagggccgg
      121 gcgcacatag aaggcgtccg gatgctcgtc gatcagcagg cgctcgccgc cgctgacggt
      181 tccgccgtcg gcccggacct gatccatggc cgccaggaag gcgtcatagg cggccttgtc
      241 atggagcggg cccagcagca caccggtctg gcgcggatcg ccgaccggcg atcgtgatcg
      301 acgacgagct cgagggcgag gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn ngcgtcgcag ggaggtgcag cgctgaccgg ctgtgcccgc cgccgaaaac
      421 acgatggcgc ggacggccag ggagaggtcg gcgctggggg tgacgatcat ggcgttgttg
      481 ccgcccagct ccaggatcga gcggccaaac cggccggcca cgatcggggc cacggcctga
      541 cccatgcggg tggagcctgt ggcgctgacc agcgggatgg ccgggtcacg caccagccgc
      601 tcgccggcgt cgcggccgcc cagcacgacc gagcacagac cttcgggcgc gtcgccgaag
      661 cgggcgaggg cgcgctccag aatggtgtgg gtggcgatgg ccgtcagcgg cgtcttctcc
      721 gacggcttcc agatcacagg gttgccgcag accagcgcca gggccgcgtt ccaggcccag
      781 accgcgaccg ggaagttgaa ggcgctgatc accagaaccg ggcccagcgg atgccaggtc
      841 tcgcgcatgg cgtggccggg acgttcggag gcgatggtca ggccatagag ctggcgcgac
      901 aggccgacgg cgaagtcgca gatgtcgatc atctcctgaa cctcgccggc cgcctcggac
      961 gcgatcttgc cggcttccag ggtgacgatc gcggccagct gatccttggc ggcccgcagc
     1021 tcctcgccca gcaggcggac cagttcgccg cgacgcgggg cgggcacggt gcgccactcg
     1081 cggaaggctt tggcggcggc cgagaccttg gcgtcgatcg cccgggcgtc gtcataggcc
     1141 gcctgggtaa ggatcgagcc atcgatgggg cagcgcacgg gcacgccgcc atcggcgggc
     1201 aggccagcga cgcccaggtc ggccagaaca gcgcgagcgt gagcggcggg ggacgggagg
     1261 gactgggtca tgcctctgga aaacgccgac ctgcgctccg ggtcaagcgc cggctgcttg
     1321 acgcgtctgg cgggcgccgt caaatgatcc ggcccgcatg ggggcgcttg ggcatgtcgc
     1381 gatcacgaat ggacgacaac gccgcctata ttcagcgact tgaggccttg agggccggag
     1441 tcgaccgacg cctggccgag ctggcgcccc ggacgggcac ggcccccgat cgccttgtcg
     1501 aggccgtgcg ctatgccttg ctggcccctg gaaaacgctt ccggccgatg ctgaccctgc
     1561 tgaccgccgc ccagtttggc gcgggcgagg gcgcagccct ggataccgcc tgcgccttcg
     1621 agatggtcca tgcctcgtcg ctgattctgg acgacctgcc ctcgatggac gacgccggcc
     1681 tgcgccgggg cctgcccacc atccatcggg cgttcgacga ggccaccgct gtgctggccg
     1741 gggtcggcct gctgaaccag gcctatgccg tggtcgccgc cgacacgcgc ctcgagagcg
     1801 ctatgcgggc cgagatcgcc gggcggatcg ccgactcggt cggcttctgc ggcctgatcg
     1861 ccggccaggc ccgtgacctg ttcgatcgcg accagctgcg cgacctgccg gctctggacc
     1921 gcctgaacca cgaaaagacc ggcgtgctga tcatggccgc cgcccagtcc ggcgcccgga
     1981 tcgccggggc ttcggatgac gccgtcgcgg cggtgggcga atttggtcgc cgcatgggcc
     2041 tggccttcca gatccgcgat gacctgatcg acgccgaggg ctcggtcgat ggggccggca
     2101 aggatgtcgg caaggacgag ggcatgacca cggccgtctc tctgctgggc gtcgccggag
     2161 cccgggccgc catggaggcg cacctcgcag ccggccgggc ggcgctcgag tccgtgggcg
     2221 gctgtgacct cgtggccggc tatgtcggcc gcctcttcgc cggacgcaag gcggcggcgt
     2281 gaccgcgtct cccattctga cggtgacggg cgtcgctcac gcctatggcg acaaggccgt
     2341 gctgcacggc gtcgacctgg ccctgtcgcc tggcgagatc tacgccctgc tgggccccaa
     2401 cggggccggc aagacgaccc tgatcaagac catctgcgga cgcttccgcc ccgatcgcgg
     2461 cgaggttcgc ctgaacggcc aggacccggc gcgggtgaac gcggcgcggg gcagcctcgg
     2521 gctcgtcccg caggagatcg ccctctacgc ccacctgacc gtcgccgaaa acctggccgc
     2581 cttcgccagc ctggc
//
LOCUS       DAOMEO010000395         2593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4269_length_2593_cov_3.489756, whole genome shotgun sequence.
ACCESSION   DAOMEO010000395 DAOMEO010000000
VERSION     DAOMEO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_2593_cov_3.489756"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1608
                     /locus_tag="PLH31_09590"
     CDS             211..1608
                     /locus_tag="PLH31_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR89496.1"
                     /translation="MSWTRRKPMEAMMPQGGGHSMARTLSWPHLLALGVGAIVGTGIL
                     TLIGVGADRAGPAVLISFAIAGAICACAALAYAELSTMMPAAGSAYSYSYVVLGEGIA
                     WIVGWSLILEYSLVVSTVAVGWSGYAAPLLTPLGFPEALTKGPELGGLINIPAIFIIG
                     VVAALLMFGTRESARLNTLLVLIKIATLSLFVWVALPAFDAQHLEPFMPFGFAKHMGP
                     DGIERGVMAAAAIIFFAFYGFDAISTAAEEAKNPDRDLAIGIVGSLIVCTLIYVLVAA
                     AAVGAMPFTRFADSPEPLALILREMGQGSIARIVAIAAVIALPTVLLGFLYGQSRIFL
                     VMARDGFLPQSLARISKRGTPARITAVTAVLVAIIAGVLPIEQIAALANAGTLIAFTA
                     VGACLLILRRRAPDLKRPFRTPAATLVGLGAVLGCAYLFLSLPMKTIYACLIWNAIGL
                     VVYFLYGQKRASAAR"
     gene            complement(1689..>2593)
                     /locus_tag="PLH31_09595"
     CDS             complement(1689..>2593)
                     /locus_tag="PLH31_09595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89497.1"
                     /translation="LCLLIFWIGCLQVMLDIGRDHDWFSDPLILTLAILAGIGFIVFI
                     IWELTEEHPIVDLRVFRHIGFSSGVFTLALAFGAYFSSIVVIPQWLQMSMGYTATWSG
                     IVTAFTAMAAVMSAPFAARFTGKVDPRIMISGAVFWLGCMTLWRAHWTSGIDFFGLAL
                     PQFVQGFAMPFFMIPLTTLTLGSVPPSETASAAGLQNFLRTMAIAIATSLVLTGWSDS
                     QRVSRNELAGALQPDDAQRALSGMGMTTEQIRQTISNLVEQEAIAISVDYVFFISALV
                     LFAAAVIVWLAPKPSGPVDTSAAH"
BASE COUNT          438 a          927 c          784 g          444 t
ORIGIN      
        1 ccgtccaatc tccgccaagc atgctcaagc ttcacgattt ttgcaacaga gccgtcgcca
       61 ttgcgttgac ctcccgctct ttggcaacaa tagcgcagca accaggccca atattgtaag
      121 aagtcgccac gttccggctc gacgcttgcg cccgcccgtc gtttgcgcca gcaaacccgc
      181 cacagaccgc ataacagggg gagcttttct atgagttgga cacgtcgcaa gccgatggag
      241 gcgatgatgc cccagggagg cggccacagc atggcgcgca ccctgtcctg gccgcatctg
      301 ctggcgctgg gcgtcggcgc gatcgtcggc accggcatcc tcaccctgat cggcgtcggc
      361 gccgatcgcg ccggccccgc cgtcctcatc tccttcgcca ttgccggcgc gatctgcgcc
      421 tgcgccgcgc tcgcctatgc cgaactgtcg accatgatgc cggcagcggg cagcgcctac
      481 agctactcct atgtggtgct gggcgagggc attgcctgga tcgtgggctg gagcctgatc
      541 ctcgaatatt cgctggtcgt ctcgaccgtg gcggtcggct ggtcgggcta tgccgccccg
      601 ctcctcaccc cgctcggctt tcccgaggcg ctgaccaagg ggccggagct tggcggcctc
      661 atcaacatcc ccgccatatt catcatcggc gtcgtcgctg ccctgctgat gttcggcacg
      721 cgcgaaagcg cccggctcaa cacgctgctg gtgctgatca agatcgcgac cttgagcctc
      781 ttcgtctggg tcgccctgcc cgccttcgac gcccagcatc tcgaaccctt catgcccttc
      841 ggctttgcca agcatatggg gcctgacggc atcgagcgcg gcgtgatggc cgccgccgcg
      901 atcatcttct tcgccttcta cggcttcgac gcgatctcga ccgcggccga ggaagccaag
      961 aatccggacc gagacctggc catcggcatc gtcggctcgc tgatcgtctg caccctcatc
     1021 tatgtgctgg tcgccgccgc agcggtgggc gccatgccct tcacccgctt tgccgacagc
     1081 ccggagccgc ttgccctcat cctgcgtgaa atgggccagg ggtcgatcgc ccggatcgtc
     1141 gcgatcgccg ccgtcattgc gctacccacc gtgctgctgg gcttcctcta cggccagagc
     1201 cgcatcttcc tggtgatggc gcgcgacggc ttcctgccgc aatcgctcgc ccgcatctcg
     1261 aagcgcggca cccctgcgcg catcaccgcc gtcacggccg tgctggtggc gatcatcgcc
     1321 ggcgtgctgc cgatcgagca gatcgccgcg ctcgccaatg ccggcacgct gatcgccttc
     1381 accgcagtgg gcgcctgcct gctgatcctg cgccgtcgcg cgccggacct caagcgcccc
     1441 ttccgcaccc ccgccgcgac gctggtgggc ctgggcgccg tgctcggctg cgcctatctg
     1501 ttcctcagcc tgccgatgaa gaccatctat gcctgcctga tctggaacgc gatcggcctc
     1561 gtcgtctatt tcctctatgg acagaagcgc gcctcggccg cccgctaggg ccaaggtccg
     1621 cacaaagaaa aagcctcccg tcgcgacagc aacaggaggc tttttctttg cctcgaacag
     1681 ggcagcgctc agtgcgccgc cgacgtgtcc accggcccgc tgggcttggg cgccagccag
     1741 acgatcaccg ccgccgcaaa cagcaccagc gcggagatga agaagacata gtcgaccgaa
     1801 atggcgatcg cctcctgctc caccagattg gatatggtct ggcggatctg ctcggtcgtc
     1861 atccccatgc ccgacagcgc gcgctgcgca tcgtcgggct gcagggcgcc cgcgagttcg
     1921 ttgcgcgaca cgcgctggct gtcgctccac ccggtcagca ccagcgacgt cgcgatcgcg
     1981 atcgccatgg tccgcaggaa attctgcagg cccgccgccg acgccgtctc gctcggcggc
     2041 accgacccca gggtcagcgt cgtcagcggg atcatgaaga agggcatggc aaagccctgc
     2101 acgaactgcg gcagcgccag cccgaaaaag tcgatgccgc tggtccagtg cgcccgccac
     2161 agggtcatgc agcccagcca gaagaccgcg cccgaaatca tgatgcgcgg atcgaccttg
     2221 ccggtgaagc gggccgcgaa cggcgcgctc atcaccgccg ccatcgccgt gaaggccgta
     2281 acgatgcccg accaggtcgc ggtatagccc atcgacatct gcagccattg cgggatgacc
     2341 acgatgctgg agaaataggc gccgaaggcc aaagccagtg tgaagacgcc cgatgagaag
     2401 ccgatatgtc ggaacacccg caggtcgacg atcggatgct cctcggtcag ctcccagatg
     2461 atgaagacga tgaagccgat gccggccagg atggcgagcg tcaggatcag cgggtcggag
     2521 aaccagtcat ggtcgcgacc gatgtccagc atcacctgca ggcagccgat ccagaagatc
     2581 agcagacaca ggc
//
LOCUS       DAOMEO010000396         2592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4274_length_2592_cov_2.690185, whole genome shotgun sequence.
ACCESSION   DAOMEO010000396 DAOMEO010000000
VERSION     DAOMEO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4274_length_2592_cov_2.690185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1212)
                     /locus_tag="PLH31_09600"
     CDS             complement(16..1212)
                     /locus_tag="PLH31_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HQR89498.1"
                     /translation="MTAHRRLIERLRDDDRGAVAVQFAFLALPIAILAFGLLDMNRIS
                     VQRRQLQDAMDAATLMAARSTATTDAALDTVGDAAFAAEMSGLGLTLTTASTTFKSGT
                     GNKVIGTASVTVTPIIANLWTQGDTTVTASSEVVRSVNKLEVALVLDNTGSMGSKLGS
                     GDKKIDALIDASKDLVDTLSAAAARASETDAVKIAVVPFSMTVNVGSTYSSANWITGT
                     MPTSSDYGDDVFGSAQNRFTLLTQTGLSWAGCVESRPDPYDISDAAPNSATPASMFIP
                     YFAPDEPDDDKVTKSGSNKYRDARTNWWGEIYNNWITDDKTKSKDWFTRQGNTSKYAA
                     NNKSKVISDAKNGTNYGPNAGCGITSVLRMTNVRTSAGATTVKNKLDDMVATGNTNVA
                     MGTVWG"
     gene            1346..2083
                     /gene="trmD"
                     /locus_tag="PLH31_09605"
     CDS             1346..2083
                     /gene="trmD"
                     /locus_tag="PLH31_09605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQR89499.1"
                     /translation="MPFTATVMTMFPEAFPGPLGVSMIGTAWKEQDLWRLETLDIRAF
                     SKDKRGFLDDTPAGGGAGAVLKADVIAAALDSVDVGGRPLLYMSARGRPLTQARVRQW
                     SAAPGIIVLCGRFEGVDQRVLDARGFEEVSVGDAVLAGGEAAAMVVIEACVRLAPGVL
                     GNIESTLEESFEDGLLEHPQYTRPRTFEGLDIPEVLLSGDHKKVAQWRSSMREETTRE
                     RRPDLWEAHLANQQAKGGPTKGKPKGD"
     gene            2087..2482
                     /gene="rplS"
                     /locus_tag="PLH31_09610"
     CDS             2087..2482
                     /gene="rplS"
                     /locus_tag="PLH31_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQR89500.1"
                     /translation="MAINIIKQLEQEESARLLAVRAIPDFQAGDTLRVNVKIKEGERE
                     RVQAYEGVCIARAGAGIHESFTVRKISFGEGVERLFPLLSPNIESIEVKRRGVVRRAK
                     LYYLRDRRGKSARIAERQTIRKEKGEAAE"
BASE COUNT          432 a          787 c          900 g          473 t
ORIGIN      
        1 gggcgacacc atgtgtcagc cccagaccgt gcccatggcg acattggtgt tgccggtggc
       61 gaccatgtcg tcgagcttgt tcttgacggt ggtggcgccg gcgctggtgc gcacattggt
      121 catgcgcagc acgctggtga tgccgcatcc ggcgttgggg ccgtagttgg tgccgttctt
      181 ggcgtcgctg atcaccttgg acttgttgtt ggccgcgtac tttgacgtgt tgccctggcg
      241 cgtgaaccag tccttggatt tggttttatc gtccgtgatc cagttgttgt agatctcgcc
      301 ccaccagttg gtccgggcgt cgcgatactt gttcgaaccg gactttgtca ccttgtcatc
      361 gtcgggctcg tcgggcgcga aatagggaat gaacatcgag gcgggcgttg cgctgttggg
      421 cgcggcgtcg ctgatgtcat aaggatctgg ccggctctca acgcagcccg cccagctcag
      481 acccgtctgg gtgagcaggg tgaaacggtt ctgcgcgctg ccgaagacat cgtcgccata
      541 gtcgctggag gtcggcatcg tgcccgtgat ccagttggcc gatgaatagg tcgagccgac
      601 attgacggtc atcgagaacg gcacgacggc gatcttcacc gcgtcggttt ccgaggcccg
      661 ggcggcggcg gcggacaggg tgtcgaccaa gtccttggag gcgtcgatca gggcgtcgat
      721 cttcttgtcg ccagacccca gcttgctgcc catcgagccg gtattgtcca gcaccagggc
      781 cacttccagc ttgttgaccg accgcacgac ctcagagctt gcggtgacgg ttgtgtcgcc
      841 ctgggtccag aggttggcga tgatcggcgt cacggtcacc gaggccgtac cgatcacctt
      901 gttgcccgtt ccggacttga aggttgtgct ggcggtcgtc agggtcaggc ccaggcccga
      961 catttcggcg gcgaaggcgg cgtcgcccac ggtgtcgagc gccgcgtcgg tggtcgccgt
     1021 cgaacgggcg gccatcaggg tggcggcgtc catggcgtcc tgcagctgcc ggcgctggac
     1081 gctgatgcgg ttcatgtcga gcaggccgaa ggccaggatg gcgatcggca gggccaggaa
     1141 ggcgaattgc acggccaccg cgccgcgatc gtcgtcgcgc aggcgttcga tgaggcggcg
     1201 gtgagcggtc atggcggagt ccgggcgatt tgcagccctc accctgccgc aggagggtga
     1261 acaagccccc aacgcgcatg gtgaagacct gcccggcgcc ttgccgcgct tcgacaattg
     1321 cggtgcgggc ggctaaacag cgcccatgcc gttcaccgcc accgtcatga ccatgttccc
     1381 cgaggccttt cccggcccgc tcggcgtctc gatgatcggg accgcctgga aggagcagga
     1441 cctgtggcga ttggaaacac tggacattcg ggccttttcc aaggataagc gcggcttcct
     1501 cgacgacacc cctgcgggtg gcggcgcggg agcggtactc aaagcggacg tcatcgccgc
     1561 cgcgctggac agcgtggacg tcggtgggcg gccgcttttg tacatgagcg cgcggggtcg
     1621 gcccctgacc caggcgcgtg ttcggcaatg gagcgcggcg cccgggatca tcgtcctgtg
     1681 cggccgtttc gagggggtgg accagcgggt gctcgacgcc cgggggttcg aggaggtctc
     1741 ggtcggagac gcggtgctcg ccggtggcga ggcggcggcg atggtcgtga tcgaggcgtg
     1801 cgttcgactt gccccgggtg tgctggggaa catcgagagc acgctggaag aaagcttcga
     1861 ggacggcctc ctcgagcatc cgcagtacac gcgaccgcgg acgttcgaag ggctggatat
     1921 ccccgaggtg ctgctgtcgg gcgaccacaa gaaggtggcc cagtggcggt ccagtatgcg
     1981 tgaagagacc actcgcgagc ggcgacccga tctctgggaa gcgcatctcg ccaatcaaca
     2041 ggcaaaaggc ggcccgacca agggtaagcc caaaggagac tgaaacatgg cgatcaacat
     2101 cattaagcaa ctcgagcagg aagaatccgc gcgtctgctg gccgtccggg cgatccccga
     2161 cttccaggcc ggcgacaccc tgcgcgtcaa cgtgaagatc aaggaaggcg agcgcgaacg
     2221 cgttcaggcc tacgagggcg tctgcatcgc ccgcgccggc gccggcatcc atgagagctt
     2281 cacggtccgc aagatctcgt tcggcgaagg cgtggagcgt ctgttcccgc tgctgtcgcc
     2341 gaacatcgaa agcatcgaag tcaagcgtcg cggcgtcgtc cgtcgcgcca agctgtacta
     2401 cctgcgcgac cgtcgcggta agtcggcccg gatcgccgaa cgccagacca tccgcaagga
     2461 aaagggcgaa gccgccgaat aggccttcga ccagaagatt tgaacgaggc cccggtggaa
     2521 acaccggggc ctttttcttt gaggctttca gccaatccgg gcgagatcgc cctttagtgc
     2581 gttcgcacgc tt
//
LOCUS       DAOMEO010000397         2590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4279_length_2590_cov_3.174359, whole genome shotgun sequence.
ACCESSION   DAOMEO010000397 DAOMEO010000000
VERSION     DAOMEO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4279_length_2590_cov_3.174359"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLH31_09615"
     CDS             <1..691
                     /locus_tag="PLH31_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQR89501.1"
                     /translation="CSRREAEALIADGLVSIDGETVEDVGRKILQGQTLVLADRATDK
                     LGASLTVLIHKPVGFVSAQPEAGEVPAARLLTRANLWLGEGPIPGSGDLLPPIGRLDK
                     DSRGLLLLSEDGVVAKAVIGPQSDLEKEYRVAVMGEITEEKLELLRFGLELDGRELRP
                     AKVEMISDQRFSIVLQEGRNRQIRRMCELVELKVVDLFRVRVGTLTLDDMPEGYWRHI
                     TPQERAALIAG"
     gene            753..950
                     /locus_tag="PLH31_09620"
     CDS             753..950
                     /locus_tag="PLH31_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89502.1"
                     /translation="MARSKLEYCLTRVVTHGLGQAIGRVASLQAHYPGITDHVHLEVM
                     ELGGDRVNAAALITSKLVVRA"
     gene            complement(1219..1743)
                     /locus_tag="PLH31_09625"
     CDS             complement(1219..1743)
                     /locus_tag="PLH31_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR89503.1"
                     /translation="MLLATAQAHDLPAIAALVNAAYRGPSAQKGWTSETHMLDGQRTD
                     VETLRADLAAKPGSTILILRETTTAPPLACVWLEPLRGDVWMIGMVTVDPERQDSGLG
                     RKLLDAAESHARAHGGKTSKMTVIEGRDTLIAWYGRRGYQPTGESEPFPYGDARFGEP
                     KLEGLKFLVLKKTL"
     gene            complement(1771..>2590)
                     /locus_tag="PLH31_09630"
     CDS             complement(1771..>2590)
                     /locus_tag="PLH31_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQR89504.1"
                     /translation="EMSGVFPASVIQIVGEQMLRLASQEQAKLGAAFAVSLLLSVWSA
                     NASMQALFDGLNIAYDEDERRNIVTRTALTYVFTFCAVLYATAAAVILIATPVLLERA
                     RLSALIEIWAPIRWMLVWLITAGAFAILYRFAPCRARARWRWVVLGSLLASAGWLVGS
                     LAFSVYVSRVAHFDATYGPLGAVIAFMIWVWFSIMAVLVGAELNAEVEHQTAFDSTTG
                     PERPMGERGAAMADTVGLAFHPWEMIKRETGVVRQAAGRAWGKVRKSGQAPPEG"
BASE COUNT          436 a          854 c          902 g          398 t
ORIGIN      
        1 ctgctcgcgt cgcgaggccg aggccctgat cgccgacggc ctggtctcca tcgacggcga
       61 gacggtcgag gatgtgggcc gcaagatcct gcagggccag accctggtcc tggccgaccg
      121 cgcgaccgac aagctgggcg caagcctgac cgtgctgatt cacaagccgg tcggtttcgt
      181 ctcggcccag cccgaggccg gcgaggtgcc cgccgcgcgc ctgctgaccc gcgccaatct
      241 gtggctcggc gaggggccga tccccggcag cggcgacctg ctgcccccga tcggccgtct
      301 ggacaaggac tcgcgcggcc tgctgctgct gtcggaagac ggggtcgtgg ccaaggccgt
      361 gatcggtccg cagtccgatc ttgaaaagga ataccgtgtc gcggtgatgg gcgagatcac
      421 cgaagagaag ctggagttgc tgcgctttgg tctcgagctg gacggccgcg aactgcgccc
      481 ggccaaggtg gagatgatct cggaccagcg gttcagtatc gtcctgcagg aaggccgaaa
      541 ccgccagatc cgccggatgt gcgaactggt tgagctcaag gtcgttgatc tgttccgcgt
      601 ccgggtcggg accctcaccc tggacgacat gcctgagggc tactggcgcc acatcacacc
      661 gcaagagcgc gcggcgctga tcgcgggtta ggcgcagtca ttcttgcgat agccggggat
      721 attggcgccg cctgcaaagt ctttcagcga gcatggcgcg ttcaaagcta gagtattgcc
      781 tgacgcgcgt cgtaacacat ggcctgggcc aggccattgg ccgcgtcgcg agcctgcagg
      841 cccactatcc cggcatcacc gaccacgtgc acctggaagt catggagctc ggcggcgacc
      901 gcgtgaacgc cgcggcgctg atcacctcga agctggtggt gagagcctag gccgggaccg
      961 ctctccgccg cgccaggatc atcgccccga cgacgaaggc gccaaagagg ctgccctcaa
     1021 gagcgcccgt gacggtctgg ctgaccggac cgaaaccgct ctccccgagc agggcgccaa
     1081 tctggtcgag gcgcaggcgc tagccctgga actggcggat cagcagatca aggctcccgc
     1141 ccatcatcct tccgcccagc agggggatca gcgcgccggc tccggcccgc ggcggccatg
     1201 gcaatcgtca tggcagtgtc agagcgtctt cttcagcacc aggaacttca gaccttcgag
     1261 cttgggctcg ccaaagcgcg cgtcgccgta ggggaagggt tcagactcgc cagtgggctg
     1321 gtagccccgg cggccgtacc aggcgatcag ggtgtcgcgt ccctcgatga cggtcatttt
     1381 gctggtcttg ccgccatgag cgcgggcatg gctttcggcc gcgtccaaca gcttgcgacc
     1441 aagcccgctg tcctggcgtt ccggatcgac ggtgaccatg ccgatcatcc acacgtcacc
     1501 ccgcaggggc tccagccaga cgcaggccaa gggcggggct gtcgtggttt cgcgcaggat
     1561 caggatggtc gagcccggct tggccgccag atcggcgcgc agggtttcca cgtcggtgcg
     1621 ctggccgtcc agcatatggg tctcgctggt ccagcccttt tgggcgctcg ggccccgata
     1681 ggccgcgttg acgagggcgg cgatggcggg gaggtcgtgg gcctgggcgg tggcgagcag
     1741 catgcggagc tccggaagcg ggccggcaag ctagccttcc ggcggcgctt gtccagactt
     1801 gcgcaccttg ccccaggccc ggccggcggc ctggcgcacg acgccagttt cgcgcttgat
     1861 catctcccag ggatgaaacg ccagaccgac cgtatcggcc atggccgcgc cgcgttcgcc
     1921 catgggccgc tccgggccgg tggtggagtc gaaggcggtc tggtgctcga cctcggcgtt
     1981 cagttctgct cccaccagca cggccatgat cgagaaccac acccagatca tgaaggcgat
     2041 caccgcgccc agcggcccgt aggtggcgtc gaaatgggcc acgcggctga catagaccga
     2101 gaaggccaga gagccgacca gccagccggc ggacgccagc agggagccca gcaccaccca
     2161 gcgccagcga gcccgggccc ggcagggggc gaagcgatag aggatcgcga atgcgccggc
     2221 ggtgatcagc cagaccagca tccagcggat cggcgcccag atctcgatca gggctgacag
     2281 gcgcgcgcgc tccagaagaa cgggtgtggc gatcaggatc accgcggcgg ccgtcgcgta
     2341 gagcacggcg cagaacgtga agacataggt cagggcggtg cgggtgacga tgttgcgtcg
     2401 ttcgtcctcg tcataggcga tgttcagccc gtcgaagagc gcctgcatgc tggcgttggc
     2461 gctccagacc gacagcagca ggctgacggc gaaggccgcg cccagcttgg cctgctcctg
     2521 gctggccagc cgcagcatct gttcgccgac gatctggatg acgctagcgg gaaagacgcc
     2581 ggacatctcg
//
LOCUS       DAOMEO010000398         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4283_length_2589_cov_2.820442, whole genome shotgun sequence.
ACCESSION   DAOMEO010000398 DAOMEO010000000
VERSION     DAOMEO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4283_length_2589_cov_2.820442"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLH31_09635"
     CDS             complement(<1..675)
                     /locus_tag="PLH31_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQR89505.1"
                     /translation="MSEDLGFLFALLLNGASIGLMYSLIALGFVLVYKATDAINFAQG
                     EFVMVAGLIVAMLLSFESVPLAVAVVVTLAVMIGFGFGLERVVLRPLLGRPVVAVIMA
                     TIGLAAILRGLGPVIFGSETRAVSLPIGDEPIVFGPASLPPIQVAGAVVAIVFFLAFN
                     WFFKKSRMGVAMRAVADNQQVAQAMGINVERYFALAWAMAGVVSALGGVVWGSMLGVD
                     VHLALVG"
     gene            complement(685..>2589)
                     /locus_tag="PLH31_09640"
     CDS             complement(685..>2589)
                     /locus_tag="PLH31_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR89506.1"
                     /translation="PKLVARNAAANPGGAGMREKTRGVWQTYSWTAYRDNVQDFALGL
                     ASLGFGRGDKLSVIGDNRPQLYFAQLSAQALGGISVPVYQDSIASELVYVLNHAETSV
                     IVAEDQEQVDKALSLKDQLPRLRSIVFDDPRGMWAYADPILCSFESVMEAGRAFGRAN
                     PDYCAREVAKGKADDTAMIAYTSGTTGAPKGAMLSHRNMIATAEAFIEANEIKAGDDW
                     LSYLPMAWVGDAAFSLGMALVAKATANCPENPETVQRDLRELGPDAVLAPPRIWENML
                     TTMQVKSGDASPLKRRTFEHFRALAERCELKRTDGKPLTLFERTGLALGEILVYGPVR
                     DQLGVRNARWCYTGGAPLGPDTYRFFRSFGINLKQIYGATEATAMIACQADAEANPNT
                     VGRPMPRVEVKIDDRGEVLLKGSNVFSGYYKQDDVTRETMTSDGWLKTGDAGFFDKQG
                     HLVIIDRAKDVGKLADGSAFAPQFIENKLKFSPFIREAVAFGDQRPFVAAMIAIDMQT
                     VGTWAEKRGIAYTSFMDLSRKDEVASLIGAEIAKANATLPDMQQARRYLLLNKELEAD
                     DAEMTRTRKVRRKFVAEKYANVIDAFYGGENSAEVTVEITFEDGRKSTMTSIIAIHDL
                     VSAPPVRRAA"
BASE COUNT          485 a          851 c          798 g          455 t
ORIGIN      
        1 gccgaccagc gccagatgga cgtcgacccc gagcatgctg ccccagacga ctccgcccag
       61 cgccgacacg acgccggcca tcgcccaggc gagcgcgaag tagcgctcga cgttgatgcc
      121 catggcctgc gcgacctgct gattgtcggc cacggcgcgc atggcaacgc ccatccggct
      181 cttcttgaag aaccagttga acgccagaaa gaagacgatc gccacgacgg cgcccgcgac
      241 ctggatgggc ggcaggctgg ccggcccgaa gacgatcggt tcgtcgccga tgggaagcga
      301 cacagcccga gtctcactgc cgaaaatgac cggacccagt ccccgcagga tggcggcaag
      361 accgattgtc gccatgatca ccgcgacaac ggggcggccc agcagcggac gcaggacgac
      421 gcgctccaat ccgaagccga agccgatcat cacggccagc gtcacgacga ccgccacggc
      481 aagtggaacg ctttcgaagg aaagcaacat cgccacgatc aggccagcca ccatgacgaa
      541 ctcgccttgc gcgaagttga tggcgtcagt cgccttgtag acgagcacga atcccagggc
      601 gatcagcgag tacatcaacc cgatcgacgc gccatttagc aacaacgcaa agagaaagcc
      661 cagatcttcg gacatcgcct ctcctcaagc cgcccgccgc accggcgggg ccgatacgag
      721 gtcgtggatg gcgatgatgg atgtcatggt cgacttgcga ccatcctcaa aggtgatctc
      781 cacggtgacc tcggcgctat tctcaccgcc ataaaaggcg tcgatgacgt tggcgtattt
      841 ctcggccaca aatttgcggc gcacttttcg agtgcgcgtc atctcggcgt cgtcggcctc
      901 gagttccttg ttaagcagca ggtaacgcct ggcctgctgc atgtctggca gcgtcgcatt
      961 ggccttggca atctcggcac cgatcaggga cgcgacctcg tctttgcggc tgaggtccat
     1021 gaagctggtg taggcgatgc cccgcttctc ggcccaggtc ccgacggtct gcatgtcgat
     1081 ggcgatcatc gccgccacaa acggccgctg gtcgccgaag gccacggcct cgcggatgaa
     1141 ggggctgaac ttcagcttgt tctcgatgaa ctgcggcgca aaagccgagc cgtcggcgag
     1201 ctttccgaca tccttggcgc gatcaatgat gacgagatgg ccctgcttgt cgaagaaccc
     1261 ggcgtcgccg gtcttcagcc agccatcgga cgtcatggtc tcccgcgtca cgtcatcctg
     1321 cttgtagtag ccgctgaaga cattggagcc tttcagcagg acctcgccgc ggtcgtcgat
     1381 tttgacctcg acgcgcggca tcggccggcc gaccgtattg gggttggcct ccgcgtcggc
     1441 ctggcaggct atcatcgccg tggcctcggt ggctccgtag atctgcttca ggttgatgcc
     1501 gaaggagcgg aagaatcggt aggtatcggg tccgagcggc gcgccgccgg tgtagcacca
     1561 gcgcgcgttg cgcacgccga gctgatcgcg caccggcccg taaacaagga tctcacccaa
     1621 ggcgaggccc gtgcgctcaa aaagtgtcaa aggcttacca tcagtccgct taagttcgca
     1681 gcgctcggct aatgcacgaa aatgctcgaa ggtgcgccgc ttgagcggcg aggcgtcgcc
     1741 tgacttcacc tgcatcgtgg tcagcatgtt ttcccagatg cgcggcggcg ccagcaccgc
     1801 gtcggggcca agctcgcgca ggtcgcgctg cacggtctct ggattctccg gacagttcgc
     1861 cgtggccttg gcgaccaacg ccataccgag cgagaaggcc gcgtcgccga cccaggccat
     1921 cggcaggtag gacagccaat cgtcacccgc tttgatctcg ttcgcctcga tgaaggcctc
     1981 ggccgtcgcg atcatgttac ggtggctcag catggcgccc ttgggcgcgc cggttgtgcc
     2041 cgatgtgtag gcgatcatcg ccgtatcgtc ggccttaccc ttggcgactt cccgggcgca
     2101 ataatccgga ttcgctcttc cgaacgctct gcccgcttcc atcacgctct cgaacgagca
     2161 caggatcgga tcggcgtagg cccacatgcc gcgcggatca tcgaacacaa tcgaacgtaa
     2221 tctgggcaat tggtccttga gcgacagcgc cttgtcgacc tgctcctggt cttcggcgac
     2281 gatcaccgag gtctcggcgt ggttcagcac atagaccagc tccgaggcga tcgaatcctg
     2341 gtagaccggc actgaaatgc cgcccagcgc ctgtgccgag agctgggcga agtagagttg
     2401 cggacggttg tcgccgatca ccgacagctt gtcgccgcgc ccgaagccta gcgacgccag
     2461 cccgagagcg aaatcctgca cgttgtcccg gtaggccgtc cagctgtagg tctgccagac
     2521 cccgcgggtc ttctcgcgca tgccggcgcc acccggattg gcggcggcgt tacgcgctac
     2581 aagcttggg
//
LOCUS       DAOMEO010000399         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4284_length_2589_cov_2.367403, whole genome shotgun sequence.
ACCESSION   DAOMEO010000399 DAOMEO010000000
VERSION     DAOMEO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_2589_cov_2.367403"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLH31_09645"
     CDS             complement(<1..336)
                     /locus_tag="PLH31_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQR89507.1"
                     /translation="MRIIALARLGLAALVLALGCAGAHEAAAQDRPAASPRGIDPVAD
                     AIAEASRRFGVPQHWIRAVMRVESAGNRTAVSRAGAMGLMQIMPGTYAELRRQYGLGP
                     DPFDVRDNIF"
     gene            complement(339..854)
                     /locus_tag="PLH31_09650"
     CDS             complement(339..854)
                     /locus_tag="PLH31_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HQR89508.1"
                     /translation="MTRAWKPLVAVSAAVALLAITAVTQPPPRLIWNASASVPIGLYA
                     ISPPRPPAAGDLVAVEPPAPLAGFLSEGGYLPPGVPLLKHVAALPGQRVCRIGRTIMI
                     DAAIVAEASLRDRRGRPLPVWSGCTTLTAGQVFLLNPGAPASLDGRYFGPLPASSIIG
                     RARPVWTREGD"
     gene            complement(851..1327)
                     /locus_tag="PLH31_09655"
     CDS             complement(851..1327)
                     /locus_tag="PLH31_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HQR89509.1"
                     /translation="MTELAAVRTRVELLHLEGKIERWIRFGEVAEEEILDRRRRALWF
                     APGSVFAFVRWASGDHGTIVSRIDILRACRAGEAYTTIPGVNPGGELLLRANGWAKVE
                     RVLVAIDQVEALGVPPTDACPDHWRHIHNRLAAADEPRPYTARRHAAWLARRSLDL"
     gene            complement(1324..1566)
                     /locus_tag="PLH31_09660"
     CDS             complement(1324..1566)
                     /locus_tag="PLH31_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89510.1"
                     /translation="MIGTRKPAFIQRPSDPEAWVRAPDRPPIGSKEAFTARLTIDVTP
                     AMRGRLKIIAFRNGQTVADMLRALLDREFPDDGDAS"
     gene            complement(1563..2201)
                     /gene="parA"
                     /locus_tag="PLH31_09665"
     CDS             complement(1563..2201)
                     /gene="parA"
                     /locus_tag="PLH31_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HQR89511.1"
                     /translation="MIVALLNQKGGVGKTTLALHLAGAWSAQGRRVLVVDADPQGSAL
                     DWAEQRVREGLPRLFGVLGLARETLHKELPELGRDLHHVVVDGPPRVAGIARSALLTA
                     DLIVIPAQPSPFDGWASAEMLRLLDEARVFRPQLRARMLLNRCAARTVIARETADALI
                     DQDPPVLASRIGQRVAFADAARTGRLVSEITGGELAAREISACAAELDKLAP"
BASE COUNT          440 a          829 c          949 g          371 t
ORIGIN      
        1 gaaaatgttg tcgcgaacgt cgaagggatc aggccccaga ccgtactggc gccgcagttc
       61 ggcgtaggtc ccaggcatga tctgcatcag ccccatggcg ccggcccggg agacggcggt
      121 gcgattgccg gcgctttcga cgcgcatgac cgcacgtatc caatgctgcg gcacgccaaa
      181 gcggcgcgag gcttctgcga tggcgtctgc cacgggatcg atgcccctcg gcgaagcggc
      241 gggacgatcc tgggcggcgg cttcatgcgc tccggcgcat cccaacgcca gaacgagcgc
      301 ggcgagaccg aggcgagcaa gggcgatgat ccgcatcgtc agtcgccctc ccgtgtccac
      361 acgggccggg cgcggccgat gatgctggag gctggcagag ggccgaagta gcgcccgtcc
      421 agcgacgctg gggcgccggg gttgagcagg aagacctggc cggccgtcag cgtcgtgcag
      481 ccgctccaga cgggcagggg acggccgcgg cgatcgcgta gcgacgcttc ggcgacgatc
      541 gctgcgtcga tcatgatcgt gcggccgatg cggcaaacgc gctgtccggg aagggcggcg
      601 acatgcttga gcagcggcac ccctggcggg agatagccgc cttcggagag aaagccggcg
      661 agcggcgctg gcggctcgac ggcgacgaga tcccccgccg caggcggccg aggcggactg
      721 atcgcgtaga ggccgatcgg cacgctggcg ctggcgttcc agatcagccg cggcggcggc
      781 tgggtgaccg cggtgatcgc aagcagcgcg acggcggcgg acaccgccac caagggtttc
      841 caggcgcggg tcataggtcc aggctccgcc tcgcgagcca cgccgcatgt cgccgcgccg
      901 tataggggcg cggctcgtca gcagcggcga ggcggttgtg gatgtgtcgc caatgatcgg
      961 gacaggcgtc tgtcggcggc acgccgagcg cctcgacctg atcgatcgcg acgagaacgc
     1021 gctcgacttt cgcccatccg ttcgcccgca gcagcagttc gccgccggga ttgacgccgg
     1081 gtatggtggt gtaggcctct cccgcgcgac aggcgcgaag gatgtcgatg cgcgagacga
     1141 tcgtgccatg atcgcccgag gcccagcgga cgaaggcgaa gacgctgcct ggcgcaaacc
     1201 acagggcgcg tcggcggcga tccaggatct cctcttcggc cacctcgccg aagcggatcc
     1261 agcgttcgat cttgccttcc agatgaagga gttcgacccg ggttctgacg gcggcgagct
     1321 cagtcatgag gcgtcgccgt cgtcggggaa ctcgcggtcg agcagggcgc gcagcatgtc
     1381 ggctacggtc tggccgttgc ggaaggcgat gatcttcagc cggccgcgca tggccggggt
     1441 gacgtcgatc gtcaggcgcg cggtgaacgc ctccttggat ccgattggag gacggtccgg
     1501 tgcgcgcacc cacgcctcgg gatcgctggg acgctggatg aaggcgggct tgcgggtccc
     1561 gatcatggcg ccaacttgtc gagttcggcc gcacaggcgc tgatctcgcg cgccgccagc
     1621 tctccgccgg tgatctcgga gaccaggcgc ccggtccgcg cggcgtcggc gaaggcgacg
     1681 cgctggccga tgcggctcgc cagcaccgga ggatcctgat cgatgagcgc gtcggccgtc
     1741 tcgcgggcga tcaccgtgcg ggccgcgcaa cggttaagaa gcatccgggc gcgcagctgc
     1801 gggcggaaga cccgggcctc gtcgagcagc ctgagcattt cggccgaagc ccagccgtcg
     1861 aaaggagagg gctgagccgg aatgacgatg agatcggcgg tgagcagagc cgagcgggcg
     1921 atccctgcga cgcgcggcgg cccatcgacg accacatggt ggaggtcgcg tccaagctcc
     1981 ggaagctcct tgtgcagcgt ctcgcgcgcc aggccgagaa caccgaacag ccgtggcaga
     2041 ccctcgcgca cgcgttgctc agcccaatcc agggctgagc cctgcgggtc cgcgtcgacg
     2101 acaagcaccc gccggccttg cgcgctccag gcaccggcca agtgtaaggc gagcgtcgtc
     2161 ttgccgacgc cgcccttctg gttgagcagg gcgacgatca tcggcgtccc cccgctggcc
     2221 ggcgcctgga ccaagcggct tcagggcgga tgccgggcgt tagaaagaat ccgtaggatt
     2281 ctaggttagg gaagttaagg ggcggacaag tcgcagaaaa ataggaatta ttccgccaat
     2341 tcggtccccg atggcacgag accgtggtcc ccgatggcac gatagtcccg gtcctcaata
     2401 gcacgagaca attcacagct tttcccccgt cgcggcgatg cgcaggcggc gaggaatggc
     2461 aaagtccagg ggatcggtgg tggggacgaa gaccagccga tcgaagccat aggggcgttc
     2521 gagcgtcagc ttgtaaccag gcagcggctg gcgccggacg atgtcgcgca ggtcgtaggc
     2581 gaaatgctt
//
LOCUS       DAOMEO010000400         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4288_length_2588_cov_2.660087, whole genome shotgun sequence.
ACCESSION   DAOMEO010000400 DAOMEO010000000
VERSION     DAOMEO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4288_length_2588_cov_2.660087"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="PLH31_09670"
     CDS             complement(<1..1151)
                     /locus_tag="PLH31_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR89512.1"
                     /translation="MTSPLARRLLAIVAVLSIAVTGVATAVAFVFVQKSAADTQMRHL
                     VEYAGERVKTEDRLFSDLVKVHNAASQALVKRLESRDAADALGEFDRLYRDHGDGTRR
                     TAPALYDGEREGPHFVYGVGGFLPKADLMSAAEKALFVSAMEVVANTGETEIHHYDNF
                     YFFTPQTRLVMFGPKRPDRLLYYRRDAPPTLDISKEEMAQITLPQNNPGRVMRCTKLR
                     RLISDPSGRGLNAACITPFYHQGRYMGSWGTSLSLDSYLLRAVADALPGATNLIVSDD
                     GDLIVSPSLAQKGVVDLGRVRHVEARHRVRELMSQIKATGQAVGAIRDRRGDRVVAFG
                     RLKEPNWWFLMTFPSDDVVWSSIKTASWILLFGVLGVVVQVMLLYRVISR"
     gene            1386..2588
                     /locus_tag="PLH31_09675"
                     /pseudo
     CDS             1386..2588
                     /locus_tag="PLH31_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
BASE COUNT          515 a          894 c          777 g          402 t
ORIGIN      
        1 cggctgatca cgcggtagag cagcatcacc tggaccacca cccccagcac gccaaacagc
       61 aggatccagg aggcggtctt gatcgacgac cacaccacat cgtctgacgg gaaggtcatc
      121 agaaaccacc agttgggctc cttgagccga ccaaaggcga cgacccggtc gccacgccgg
      181 tcgcggatcg caccaacagc ctggccggtg gccttgatct ggctcatcag ctcgcggacg
      241 cgatggcgcg cctcgacatg gcgcacccgc ccaagatcga ccacgccctt ctgggccagg
      301 ctcggcgaga cgatcaggtc gccgtcgtcc gagacgatca agttggtcgc gcccggcagg
      361 gcgtcggcca ccgcccgcag caggtaggaa tccagcgaca ggctggtccc ccaggagccc
      421 atgtatcggc cctggtgata gaagggcgtg atgcaggccg cgttcaaacc gcgaccgctg
      481 gggtcggaga tcagccggcg cagcttggtg catctcatca cccggccggg attgttctgc
      541 ggcagggtga tctgggccat ttcttccttg gagatatcca gggtcggcgg agcgtcgcgg
      601 cgatagtaca gcagcctgtc aggccgcttg ggcccgaaca tcaccagccg cgtctggggc
      661 gtgaagaagt agaagttgtc gtagtggtgg atctcggtct cgccggtatt ggccaccacc
      721 tccatggccg agacgaacag cgccttctcg gcggcgctca tcaggtcggc cttgggcagg
      781 aagccgccga caccatagac aaagtggggc ccttcgcgtt cgccgtcata cagcgccggc
      841 gcggtgcgcc gggtgccgtc gccatgatcc cggtacaggc ggtcgaactc acccagggcg
      901 tcggcggcgt cgcggctttc cagacgcttg accagggcct ggctggcggc gttgtgcacc
      961 ttgaccagat cggaaaacag gcggtcctcg gtcttcaccc gctcgccggc atattcgacc
     1021 agatgccgca tctgggtgtc ggccgcgctc ttctgcacga acacgaaggc gaccgccgtc
     1081 gccacgccgg tgacggcgat cgacagcacc gcgacgatcg ccagcaggcg gcgcgccagc
     1141 ggcgaggtca tcccggtatt gggcaacagc cttctcacgc ccggttcagc cccccgatct
     1201 ctcgccggag atcctgatca cccatcaagg cgcagcagcc ctaaatatcg cctgaagaaa
     1261 acgccattcc accgttcggc ttttcccttc acgcacaaca gtctataggc agcgcgtccg
     1321 cgccgcgaac tggtcctgtg cgccagattg cctcaggcgc gccaccgcga gaggaagacc
     1381 ccgcaatggc caagatcaaa gtcgccaatc ccgttgtcga tatggacggc gacgaaatga
     1441 cccggatcat ctggaagctc atcaaggata agctgatctt cccctatctg gacctggaac
     1501 tggactatta cgacctgtcg gtcgagaacc gcgacgccac caacgaccag gtgacgatcg
     1561 acgccgccga ggccaccaag cgccacggcg tcgccgtcaa gtgcgccacc atcacgcctg
     1621 acgagcagcg cgtcgaggaa ttcaagctca agaagatgtg gaagtcgccc aacggcacga
     1681 tccgcaacat cctgggcggc gtgatcttcc gcgagccgat catctgccag aacgtgccgc
     1741 gcctggtgcc cggctggacc cagccgatca tcgtcggccg ccacgcgttc ggcgaccagt
     1801 acaaggccac cgacttcctg ttcccgggca agggcaccct gacgatgaag ttcgtcggcg
     1861 aagacggcgc ggtcatcgag cacgagatct acaaggcccc cggcgccggc gtggccatgg
     1921 gcatgtacaa cctcgacgag tcgatccgcg acttcgcgca cgcctcgttc gcctacggcc
     1981 tgacgcgcaa ctatccggtc tatctctcca ccaagaacac catcctgaaa gcctatgacg
     2041 gccgcttcaa ggacatcttc caggagatct tcgacgccga atacgccgac aagttcaagg
     2101 ccgccggcat ccactacgag caccgcctga tcgacgacat ggtggcctcg gccctcaagt
     2161 ggtcgggcgg ctatgtctgg gcgtgaaaga actatgacgg cgacgtgcag tccgacacgg
     2221 ttgcgcaggg cttcggctcg cccggcctga tgacccccgt gcccctctcg cccgatggca
     2281 agacggtcga ggccgaagcc ccccacggca ccgccacccg ccactatcgc cagcaccaga
     2341 agggccagag cacctcgacc aactcgatcg cctcgatctt cgcctggacg cgcggcctgg
     2401 cccaccgcgc caagctggac aacaaccagg acctggcaaa cttcgccctg accctggaaa
     2461 aggtctgcgt cgacaccgtc gaaagcggct tcatgaccaa ggacctggcc ctgctggtcg
     2521 gcgacaagca gtcgtggctg accaccgaag gcttcctcga caagatcgac gagaacctga
     2581 agaaggcc
//
LOCUS       DAOMEO010000401         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4291_length_2588_cov_2.324516, whole genome shotgun sequence.
ACCESSION   DAOMEO010000401 DAOMEO010000000
VERSION     DAOMEO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4291_length_2588_cov_2.324516"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLH31_09680"
     CDS             complement(<1..374)
                     /locus_tag="PLH31_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR89513.1"
                     /translation="MTRIISGEAQPHEAVPLAADKALRPQTLAEFVGQEQAKSNLRVF
                     IEAAKGRGEALDHVLLFGPPGLGKTTLAQIVARELGVNFRATSGPVLNKPGDLAAILT
                     NLEANDVLFIDEIHRLSPAVEE"
     gene            complement(523..1140)
                     /gene="ruvA"
                     /locus_tag="PLH31_09685"
     CDS             complement(523..1140)
                     /gene="ruvA"
                     /locus_tag="PLH31_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQR89514.1"
                     /translation="MIGRLRGAVAEVGEEEALIDVMGVGYVVRCGGRTLSRLPALGEE
                     ALLHIESQWSESAGLRLYGFATREDRRAFVLLQAIQGVGPKAAMAVLDILSPAELASA
                     VAREDKAAVGRASGVGPKLALRIVTELKDKPITDGPVLMTAPSTGSAPVKAKPAPTGD
                     AVAALMGLGVAEVNARRVVEAAAAKLGEEATVQALIKAGLQELGR"
     gene            complement(1137..1658)
                     /gene="ruvC"
                     /locus_tag="PLH31_09690"
     CDS             complement(1137..1658)
                     /gene="ruvC"
                     /locus_tag="PLH31_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQR89515.1"
                     /translation="MREHMMNMNRIPIRILGLDPGLRRTGWGVVTVEGSRMSHVAHGV
                     IAPDDKADFAQRLLHLFEGICAVIEQHQPDEAAVEEVFLNTNAQSTLKLGHARAAALI
                     APARAGLLVAEYSTRLVKKAVVGTGAADKAQIGFMIARLLPTAGKTTADCADALAVAI
                     THANLRVANRRVA"
     gene            1780..>2588
                     /locus_tag="PLH31_09695"
     CDS             1780..>2588
                     /locus_tag="PLH31_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR89516.1"
                     /translation="MRLAGWMTTAALLALPTMASAQTKAPRPDQLTFRELFKELVEIN
                     TTLSVGSCTAAAEAMGARLKAAGYAEADVRVVVEPKYPREGSLVAVLRGTDPKSKPML
                     LLAHIDVVEANRADWTRDPFTLIEEDGYLYGRGTSDDKAQAAIWTDSLIRLKQAGYKP
                     NRDIKMALTCGEESEGYNGIEDLLKNHRPLVDAEFALNEGADGLLDENGKEVVLEVQA
                     GEKVYQDFTLTITNPGGHSSRPVPANAIHQLAAALSRIGAYSFPPRFNDATR"
BASE COUNT          412 a          895 c          889 g          392 t
ORIGIN      
        1 atttcctcca ccgccggcga gaggcggtgg atctcgtcga tgaacaggac gtcgttggct
       61 tcgaggttgg tcaggatcgc ggccaggtcg ccgggcttgt tcagcaccgg gccggaggtg
      121 gcgcggaagt tcacgcccag ttcgcgggcg acgatctggg ccagggtggt cttgcccagg
      181 cccggcgggc caaacagcag cacatggtcc agggcctcgc cccggccctt ggcggcctcg
      241 atgaagacgc gcagattgct cttggcctgc tcctgaccga cgaactcggc cagggtctgg
      301 ggccgcaggg ccttgtcggc ggccagcggg acggcctcgt ggggctgggc ttcgccggag
      361 atgatgcggg tcattgagcg atatcctccc ttcccgtcga cggatggggg aagggtcggg
      421 gatgggggtg actggggcgg tcaggttcgc gccagcggcc agtgccgaga cacccccatc
      481 ccaacccttc ccccatcaag ggggaagggc ttttaagcgc cctcaccgcc ccagctcctg
      541 caaaccggcc ttgatcagcg cctgcaccgt ggcctcctcg cccagcttgg cggcggcggc
      601 ctcgaccaca cggcgagcgt tcacttccgc aacgcccaga cccatcagcg ccgccacggc
      661 gtcgccggtc ggggcgggct tggccttcac cggcgccgaa cccgtcgaag gcgcggtcat
      721 cagcaccggt ccgtcggtga tcggcttgtc cttcagctct gtgacgatcc gaagagccag
      781 tttcggtccc acgcccgacg cacgcccgac ggcggccttg tcctcgcggg ccacggcgct
      841 ggccagctcg gccggcgaca ggatatccag caccgccatg gccgccttgg gccccacgcc
      901 ctggatggcc tgcagcagca cgaaggcgcg gcggtcctcg cgggtggcaa agccgtagag
      961 ccgcaggccg gcgctctcgc tccactggct ttcgatgtgc agcagcgcct cttcgcccag
     1021 ggccggcagg cgcgacaggg tgcggccgcc gcaacggaca acatagccca cgcccatgac
     1081 gtcgatcagg gcctcctcct cgccgacctc ggcgacggcc ccgcgcaggc gaccaatcat
     1141 gcgaccctcc ggttggcgac gcgcaggttg gcgtgggtga tggcgacggc cagggcgtcg
     1201 gcgcagtcgg ccgtggtctt gccggccgtg ggcagcagac gggcgatcat gaagccgatc
     1261 tgggccttgt cggccgcgcc ggtgcccacc accgccttct tgaccaggcg cgtcgaatat
     1321 tcggccacca gcagtccggc gcgagcgggc gcgatcaggg cggcggcgcg ggcgtggccc
     1381 agtttcagcg tcgactgggc attggtgttg aggaacacct cctcgaccgc cgcctcatcg
     1441 ggctggtgct gctcgatcac cgcgcaaatg ccttcgaaca gatgcagcag gcgctgggcg
     1501 aagtcggcct tgtcgtcggg ggcgatcacc ccgtgggcga cgtggctcat gcgcgagccc
     1561 tcgaccgtga ccacgcccca gccggtgcgg cgcaggccgg ggtcgagccc caggattcgg
     1621 atcgggatgc gattcatgtt catcatgtgt tctcgcaaca tcgcctgttc cgcgcccgag
     1681 gaaaagcccc tgtatcgcta acgactcgta aacgccggcc cttgcgcggc gaggctttgc
     1741 gcgctctcct cgccgcaaag atcttgggga gacgatttga tgcgactggc gggatggatg
     1801 acgacggcgg ccctgctggc cctgccgacg atggcgagcg cccagaccaa ggctccgcgc
     1861 cccgaccagc tgacgttccg cgagctgttc aaggagctgg tcgagatcaa caccaccctg
     1921 tcggtgggca gctgcaccgc ggctgcagag gcgatgggcg cgcgcctgaa ggccgccggc
     1981 tacgccgagg ccgacgtcag ggtagtggtc gagccgaaat acccccgcga aggctcgctg
     2041 gtggccgtgc tgcgcggaac cgatcccaag tcgaagccca tgctgctgct ggcccatatc
     2101 gacgtggttg aggccaaccg cgccgactgg acccgcgacc cgttcaccct gatcgaggag
     2161 gacggctatc tctacggacg cggcacgtcg gacgacaagg cccaggccgc catctggacc
     2221 gacagcctga tccgcctgaa gcaggcgggc tacaagccca accgcgacat caagatggcc
     2281 ctgacctgcg gcgaggagag cgagggctat aacggcatcg aggacctgct gaagaaccac
     2341 cgcccgctgg tcgacgccga gttcgcgctg aacgagggcg ccgatggcct gctggacgag
     2401 aacggcaagg aagtggtgct ggaggtccag gcgggcgaga aggtctatca ggacttcaca
     2461 ctgaccatca ccaatccggg cggccactcc agccggcccg tgcccgccaa cgccatccat
     2521 cagctggcgg cggcgctgag ccgcatcggc gcctattcgt ttccgcctcg gttcaacgac
     2581 gccacgcg
//
LOCUS       DAOMEO010000402         2584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4298_length_2584_cov_2.788454, whole genome shotgun sequence.
ACCESSION   DAOMEO010000402 DAOMEO010000000
VERSION     DAOMEO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_2584_cov_2.788454"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLH31_09700"
     CDS             <1..251
                     /locus_tag="PLH31_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89517.1"
                     /translation="RYSDAANTLKLPAYTLVGATVRFNVTDKLAIYAYGNNLTDEIGL
                     TEGNPRAGQIISTEAGAVYGIGRPEFGRSFRAAVMYRF"
     gene            326..1792
                     /locus_tag="PLH31_09705"
     CDS             326..1792
                     /locus_tag="PLH31_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQR89518.1"
                     /translation="MIRCLLTLLVLCLATPVWAREQAPDFVLTGQIVGGDHQTYKPVS
                     FDVPDGVTRLTVAFDYTGRDQKTVIDLGLMDPHRFRGWSGGNKRTFTLGVEDATMSYL
                     PGPLPAGRWTLLLGVPNTRAETVASFEARIFLERTPGGPSLAAKPLKAGAGWYRGDLH
                     AHTGHSDGSCQTQSGARAPCPVYRTAEAATARGLDFIAITDHNTTSHFAAMAQLQPAF
                     DRLLLIPGREVTTFHGHANVFGPTAFIDFRLGDAAVPNVRTLQSLAEGAGGFLSINHP
                     TALSGEACMGCGWTAPDTDYARVGAIEVANGGSERAQGGAEGPLLGVAFWEAQLNAGH
                     RITAIGGSDNHDAGLSSDIASSIGRPTTVVHAPELSTTGLLAGLRAGRVFIDFDGSRD
                     RLLDLSARTARSTATMGGALAARRGETVIFTATVAGADQARLEIVQDGEKRAPVFTRP
                     GVFSIRMGDRPSWVRVNLRDTDGRLLAIGNPIYLSPAP"
     gene            complement(1824..2042)
                     /locus_tag="PLH31_09710"
     CDS             complement(1824..2042)
                     /locus_tag="PLH31_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89519.1"
                     /translation="MSYDVLSMRWNARRDAEGNLIVSAHQETSIDGVYAAGDLVPGLN
                     QIAVASAEAALAATDIHRRLRALESPER"
     gene            2285..2545
                     /locus_tag="PLH31_09715"
     CDS             2285..2545
                     /locus_tag="PLH31_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HQR89520.1"
                     /translation="MLARLRRAVPETSAAPQGPGGLTETRSFGANPGALKMWTYAPPN
                     LPKGAPLVVVLHGCTQTAHGYANHAGWIALAERLGFAVLAPE"
BASE COUNT          403 a          902 c          845 g          434 t
ORIGIN      
        1 accgctactc cgacgccgcc aacaccctca agctgccggc ctacacgctg gtgggcgcca
       61 cggtgcggtt caacgtcacc gacaagctgg ccatctatgc ctatggcaac aacctcaccg
      121 acgagatcgg cctgaccgag ggcaatcccc gcgccggcca gatcatcagc accgaggcgg
      181 gcgccgtcta tggcatcggc cgtccggagt ttggtcgctc gttccgcgcg gctgtcatgt
      241 accgcttcta gttttcgttt ccagatcccc tcgacttggg cggccggttc tgccggccgc
      301 ccatcttttc gtttggaaga ctgacatgat ccgctgcctg ctgacgctgc tcgttctctg
      361 cctggcgacc ccggtctggg cccgagaaca ggccccggat ttcgtgctca ctggccagat
      421 cgtcggcggc gatcaccaga cctacaagcc cgtgagcttt gacgtaccgg atggcgtgac
      481 ccggctgacc gtcgccttcg actataccgg ccgggaccag aagacggtca tcgacctggg
      541 cctgatggat ccccatcgat tccggggctg gagcggcggc aacaagcgca ccttcaccct
      601 gggggtcgag gacgccacca tgagctacct gcccggaccg ctgccggccg ggcgctggac
      661 cttgctgctg ggcgtgccca atacccgcgc ggagaccgtg gcgtcctttg aggccaggat
      721 cttcctggag cggacgcctg gcgggccgag ccttgcggcc aagccgttga aggccggggc
      781 cggctggtat cgcggcgacc tgcacgccca caccggtcac agcgacggct cgtgccagac
      841 ccagagcggc gcccgcgcgc catgcccggt ctatcgcacc gccgaggccg ccaccgcccg
      901 gggcctggat ttcatcgcca tcaccgatca caacaccacc agccacttcg ccgcgatggc
      961 gcaattgcag ccggccttcg acaggttgct gctgatccct ggacgggagg tcaccacctt
     1021 ccacggtcac gccaatgtct tcggcccgac cgccttcatc gacttccgcc tgggtgatgc
     1081 ggccgtaccg aatgtccgaa ccctgcagag tctggcggag ggcgccggag ggttcctgtc
     1141 tatcaatcac ccgaccgcgc tttccggcga ggcctgcatg ggctgcggct ggacagctcc
     1201 cgacacagac tatgccaggg tcggagccat cgaggtcgcc aatggcggct ccgagcgcgc
     1261 ccagggcggc gctgagggac cgcttctggg tgtcgccttc tgggaggcgc agttgaacgc
     1321 cggccatcgg atcacggcca tcggcggcag cgacaatcac gatgcgggtc tgtcgtcgga
     1381 tatcgcctcg tcgatcggcc ggccgaccac cgtggttcat gcgccggagt tgtccacgac
     1441 gggcctgctc gccggcctgc gggccgggcg cgtgttcatc gacttcgacg gtagccgcga
     1501 ccggttgctg gacctctcgg ctcgcaccgc gaggtcgacg gcgacgatgg gcggcgcgct
     1561 cgcggctcgc cgcggtgaaa cggtgatctt caccgcgacg gtcgctggcg ccgatcaggc
     1621 gcggctggag attgtccagg acggcgagaa acgcgcgccg gtgttcaccc gtccgggcgt
     1681 gttctcgatc aggatgggcg accgccccag ctgggtgcgc gtcaatctgc gcgacaccga
     1741 tggtcgactg ctggcgatcg ggaatccgat ctatctgagc cccgcgccct gaccgcaggc
     1801 tccagaggct gaagacagcc gctctaacgc tcgggcgact ccagggctcg caggcgtcga
     1861 tgaatatcgg tggccgcaag ggccgcctcg gcgctggcga ccgcgatctg gttcaggcct
     1921 ggaacgaggt cgcccgccgc atagacccca tcaatcgacg tctcctgatg ggcgctgacg
     1981 atcagattgc cttctgcgtc gcgccgggcg ttccatcgca tcgatagcac gtcgtacgac
     2041 atcagggccc tccacgcggc gacggcgccg ccaggcgaaa acacgcggcg acggcgccgc
     2101 cagggcgaaa acgacgccgc gccatggagg ttcgccgcgc cccgtcaggc cctcgggttt
     2161 cggggctcga aaagacgtga tcaaacgtga tgcgtcctta tgctgcagtg cagcattctc
     2221 tttggcggac cggcttgcgg ttgtagcgag aatgatgatg ccaggccttg gtgagacgac
     2281 tgcaatgctg gcgcgcctga ggcgcgcggt ccccgagacg tccgccgcgc cccagggccc
     2341 gggcggtctg accgagaccc ggtccttcgg ggccaatccc ggcgccctga agatgtggac
     2401 ctatgcgccg cccaacctgc ccaagggggc gccgcttgtc gtggtcctgc atggctgcac
     2461 ccagacggcg cacggctatg caaaccatgc cggctggatc gccctggccg agcggcttgg
     2521 cttcgccgtc ctggcgcccg agtagtcgaa cgtcaacaat cccaagcgct gcttcaactg
     2581 gttc
//
LOCUS       DAOMEO010000403         2584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4299_length_2584_cov_2.782523, whole genome shotgun sequence.
ACCESSION   DAOMEO010000403 DAOMEO010000000
VERSION     DAOMEO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_2584_cov_2.782523"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLH31_09720"
     CDS             complement(<1..209)
                     /locus_tag="PLH31_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89521.1"
                     /translation="MNKSHLLGVSLVALIASTPAFAQVQAPSSTVEEIIVTATKREQT
                     LQDVPVSVSVTGAQADVTPISHPAIT"
     gene            389..1039
                     /locus_tag="PLH31_09725"
     CDS             389..1039
                     /locus_tag="PLH31_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR89522.1"
                     /translation="MKGEHAIATGEADGPRQLSHSRKSAKKRDAILRAAIEIINVKSF
                     ALATMSEIAATLDLRDAALYYYFPSKQALAFACHLQSLERFEGMLIAADQNGGSGAAR
                     LEHVLQLLMLDTAENGPQLYFGDLSYLTDSQRDEVSVWGLRLIGDLERFVEEGIMDGS
                     IVPCEPKLVVQLLLGMLIWLAKWVPAFKGVTPDRLMAAMGAVSLSGLMPRQPAPGL"
     gene            complement(1126..1410)
                     /locus_tag="PLH31_09730"
     CDS             complement(1126..1410)
                     /locus_tag="PLH31_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89523.1"
                     /translation="MQVKGKLKEAAGSAKQAVGHLKTVAADALGDAQHRVHDAALSAR
                     DQAKDRFSQIETQVRDKPGPALAVALVIGLGLGLLLRGPSSRTVYLRDYE"
     gene            complement(1630..2442)
                     /locus_tag="PLH31_09735"
     CDS             complement(1630..2442)
                     /locus_tag="PLH31_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR89524.1"
                     /translation="MTGPLEFTILGSGSSGGVPRADGDWGDCDPTDPRNLRTRCSLLV
                     RRKGQGGPLSETTVVVDTSPDFRLQAVAAGARRIDAALFTHDHADQAHGIDDLRPFFL
                     NQRRKIPTYMDEATHAGLTRRFEYVFETHGGYPAICEPMPLPPHGEAWSLEGPSGHIP
                     VQSFDLDHGNIRSVGFRFGDVAYTPDVAAIPEASWPALANLDVWIVDALRWTPHPTHA
                     HVDLALEWIARAKPRLAILTNLHIDLDFNRLAEKLPTGVRPAFDGMRFVRDA"
     gene            complement(2439..>2584)
                     /locus_tag="PLH31_09740"
     CDS             complement(2439..>2584)
                     /locus_tag="PLH31_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HQR89525.1"
                     /translation="MRGRRNEPAYLPHIVDKLGEIRGWTRAESEGRTEDAFFALFDRI
                     PRP"
BASE COUNT          497 a          775 c          806 g          506 t
ORIGIN      
        1 gttatcgctg gatgagaaat gggggtcacg tcagcctgcg cccccgtgac cgacaccgaa
       61 accggaacgt cctgcaaagt ctgttcgcgc ttcgtcgccg tgacgatgat ctcttcgacg
      121 gtcgagctgg gcgcttgaac ctgcgcaaat gcaggggtgc tggcgatgag cgcgacgagt
      181 gaaacgccga gcaagtgact cttattcacg tgaacctccc aatgtttttt atcgggccca
      241 cgtcgttgcg ccggccatcg ttgtccaatt ttatgagcac ctagatattt taatgcaagt
      301 tagacttttg agggcgacgc ttgcggtatc gcctttcctc gcttcaattg gggacagatc
      361 gccagcaatg ggcgactggg agggtctcat gaagggtgag catgctatcg cgacaggcga
      421 agccgatggc cccaggcagc tgtcccacag tcgaaaatca gccaagaagc gcgacgcgat
      481 cctgcgagcc gcgatcgaga tcatcaacgt caagagtttc gcactcgcga cgatgtcgga
      541 aatcgccgct acactcgatc tccgggatgc tgcgctctac tattatttcc caagcaagca
      601 ggccttggcc ttcgcctgcc acctgcaatc tctggagcgg ttcgaaggta tgctgatcgc
      661 tgccgaccag aacggcggtt ccggcgcagc cagactggaa cacgttctcc aactgttgat
      721 gttggacacg gcggagaatg gaccccagct gtacttcggc gacctttcct atcttacgga
      781 ttcgcagcgc gacgaagtct cggtgtgggg attgaggctg atcggcgatc tcgagcgatt
      841 tgtcgaggaa ggcatcatgg acggatcgat cgtcccctgt gagccgaagc tggtcgttca
      901 attgctattg ggcatgctga tctggctcgc caaatgggtg cccgcgttca agggcgtgac
      961 gcccgacaga cttatggccg ccatgggcgc ggtaagcctg agcgggctga tgccccggca
     1021 gccagccccc ggcctttgac cccaccgctg gttcaaaaac gactcggtca tcagaagcat
     1081 tgacggcgac actgagcgcc gccgtcactg caccaagcct ggcgcttact cgtagtcgcg
     1141 cagatagacg gtgcggctgc tagggccgcg gagtagaagg cccaggccaa ggccgataac
     1201 caaagcgacg gccaaagccg ggcctggctt gtcacgcacc tgggtctcga tctgggaaaa
     1261 gcgatccttg gcctggtcgc gcgccgaaag cgcggcgtcg tgaacgcgat gttgcgcatc
     1321 gcccaaggca tcggcggcga ccgttttgag gtggccaacc gcctgcttcg ccgaaccggc
     1381 cgcttctttc agcttgccct tgacttgcat ggttgcgtcg ccagtgagag cgcccgctgc
     1441 gtcctgagcc cgccctgcgc cgatctccaa cgcgccgttc aattgctcat ccatgttctg
     1501 atcccgactg gggcgcgctg ggcgccgctt taaacctgtc gcggcgatac gatccgcgcc
     1561 ctgtcgagaa ccccttgcgg cgcaaactgt tccagaccca atcgagttgg aaagatttgc
     1621 gctgacgcct caggcgtcgc gaacgaagcg cataccatca aaagctggcc tgaccccggt
     1681 tggaagtttt tcagccaacc gattgaaatc cagatcgata tgtagatttg tcagaatcgc
     1741 caggcgcggc ttggcgcggg cgatccactc gagcgccaag tcaacgtggg cgtgggttgg
     1801 atgcggcgtc cagcgtagtg cgtccacgat ccagacatcg agattggcga gcgccggcca
     1861 gctggcttca ggaatggctg ctacgtcggg cgtgtaggca acatcgccga accgaaagcc
     1921 gacagaccgg atgttgccgt gatccagatc gaagctctgg acggggatgt ggccgctggg
     1981 tccttccaag ctccaggcct cgccatgggg cggcaggggc atcggttcgc agatggctgg
     2041 atagccgcca tgcgtctcga acacatactc gaaacgccgg gtcagtccgg cgtgcgtcgc
     2101 ctcgtccatg taggttggga tcttgcggcg ttgattgagg aagaatggcc gcaaatcgtc
     2161 aatgccgtgg gcttggtcag cgtggtcgtg tgtgaagagc gcagcgtcaa ttcgccgggc
     2221 gcccgcagcg acagcctgaa gtctgaaatc cggcgaggtg tccacaacga cggtggtctc
     2281 ggaaaggggg ccgccctgcc ctttcctgcg caccaacagg gagcaccgtg tccgcagatt
     2341 gcgcggatcg gtcggatcgc aatcacccca gtcgccgtcg gcgcgcggta cgccgcccga
     2401 tgagcctgac cccaggattg tgaactccag cgggccggtc acggtcgcgg aatccggtcg
     2461 aatagcgcaa aaaaggcgtc ttcagtccgg ccttcgcttt cagcgcgtgt ccagccgcga
     2521 atttcgccca gcttgtcgac gatatgaggg agataggccg gttcattccg gcgaccccgc
     2581 atcg
//
LOCUS       DAOMEO010000404         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4303_length_2583_cov_2.724288, whole genome shotgun sequence.
ACCESSION   DAOMEO010000404 DAOMEO010000000
VERSION     DAOMEO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_2583_cov_2.724288"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..741)
                     /locus_tag="PLH31_09745"
     CDS             complement(100..741)
                     /locus_tag="PLH31_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HQR89526.1"
                     /translation="MAWTRDDMAARAAKELRDGFYVNLGIGIPTLVANYIPEGMSVTL
                     QSENGMLGMGPFPYEGEEDADLINAGKQTITELDSSSYFSSADSFAMIRGGHIALSIL
                     GGMQVSETGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVVMDHCEKSGAPKLLKSCSL
                     PLTGAGVVDLLITELGVFEINRGKTPVRLIELAPGVTVDEVKAKTEAAFEVAI"
     gene            complement(741..1466)
                     /locus_tag="PLH31_09750"
     CDS             complement(741..1466)
                     /locus_tag="PLH31_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQR89527.1"
                     /translation="MVDKVQKDAVEALSGLLFDGMTIMAGGFGLCGIPENLIAAIRET
                     GVKDLTVVSNNCGVDGFGLGILLENRQIKKMISSYVGENKLFEQLYLSGELELEFNPQ
                     GTLAERIRAGGAGIPAFFTKTGVGTLVAEGKEVREFDGELYVMERGLTADLSIVKAWK
                     ADAEGNLVYRKTARNFNPMMATAGKACVVEVEQIVAIGALDKDNIHTAGIYVDRVLKG
                     AKFEKRIERVTTRPRDEKREETV"
     gene            1608..2177
                     /locus_tag="PLH31_09755"
     CDS             1608..2177
                     /locus_tag="PLH31_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQR89528.1"
                     /translation="MTAPPAAATSGVLLVLAHPALERSRANRALAKAAKGLSGVTFRD
                     LYETYPDFAIDIETEQASLLAHDVIALQFPLYWYSTPALLKEWLDLVWLHGFAYGHDG
                     TALVGKRLFVACTTGASAKAYHAQGYNRFTMDDFLRPLEQTAHLCGMVWETPFVVHGA
                     AVKDDEALKAEAERYKARIASLLSATLEV"
     gene            2181..>2583
                     /locus_tag="PLH31_09760"
     CDS             2181..>2583
                     /locus_tag="PLH31_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR89529.1"
                     /translation="MQSFLTQTLFYLGAAVISVPIAKRLGLGSVLGYLIAGVIIGPHA
                     LSLIGAQADVMQFAEFGVVILLFLIGLEVQPSTLWDMRKAIFGLGGAQVAGTALSVAG
                     VSMLLGLPWQTALAVGLVLAMSSTAIVLQTLD"
BASE COUNT          412 a          867 c          803 g          501 t
ORIGIN      
        1 tcatgtccgt cctctcaagg gccggactct agctccgcgt ggaggaaaaa atcagaggca
       61 ggtcagcgcc gtttcgccat catggctcag cggtgtcggc tagatcgcga cctcgaaggc
      121 cgcttcggtc ttggctttca cctcatccac cgtgacgccc ggcgccagtt cgatcagccg
      181 caccggggtc ttgccgcgat tgatctcgaa gacgcccagt tcggtgatca gcagatcgac
      241 cacgccggcc ccggtcaggg gcagcgagca ggacttcagc agcttgggcg cgcccgactt
      301 ctcgcagtgg tccatcacca ccaccacgcg cttgacgccg gcgaccagat ccatggcccc
      361 gcccatgccc ttgaccatct tgcccggcac catccagttg gccaggtcgc cggtctcgga
      421 cacctgcatg ccgcccagga tcgacagggc gatgtgcccg ccgcggatca tcgcgaagct
      481 gtcggccgac gagaaatacg agctggagtc cagctcggtg atcgtctgct tgccggcatt
      541 gatcaggtcg gcgtcttctt cgccctcata ggggaagggg cccatgccca gcatgccgtt
      601 ctcgctctgc agcgtgacgg acatgccttc ggggatgtag ttggccacca gggtcgggat
      661 gccgatcccg aggtttacat agaagccgtc gcgcagctct ttcgccgcgc gggcggccat
      721 gtcatcgcgg gtccaggcca tcagacggtc tcctcacgct tctcgtctct ggggcgcgtg
      781 gtgacccgct cgatgcgttt ctcaaacttg gcacccttca gcacccggtc gacatagatg
      841 ccggcggtgt ggatgttgtc cttgtcgagc gcgccgatgg cgacgatctg ctcgacctcg
      901 acgacacagg ccttgccggc cgtggccatc atcggattga agttccgggc cgtctttcga
      961 tagaccagat tgccctcggc gtcggccttc caggccttga cgatcgacag gtcggcggtc
     1021 aggccgcgct ccatcacata gagctcgccg tcgaactcgc gcacttcctt gccctcggcc
     1081 accagggtgc cgacaccggt cttggtgaag aaggccggaa tgcccgcgcc gccggcgcga
     1141 atgcgttccg ccagcgtgcc ctgcggattg aactccagtt ccagctcgcc gctaaggtag
     1201 agctgttcga acagcttgtt ctcccccaca taggacgaga tcattttctt gatctggcgg
     1261 ttctccagca ggatccccag gccaaagcca tcgacgccgc aattgttgga gacgaccgtg
     1321 aggtccttga cgcccgtctc gcggatcgcc gcgatcaggt tctcgggaat gccgcagagg
     1381 ccaaagcctc cggccatgat ggtcatgccg tcgaacagca gccccgaaag ggcctcgacc
     1441 gcatctttct gcaccttgtc gaccatggat cctccgtcac cagcctttgg catgccgatg
     1501 tttggcgaag ggtctcgccc ggcgaccaaa gaaattcaac ccttgatgaa taaaaattcg
     1561 acgcctggag acgccgcttg agcccgatcg aaaacagggg tgacgctatc accgcccccc
     1621 cggccgccgc cacctctggc gtgctgctgg tcctggccca tcccgccctt gaacgctctc
     1681 gcgccaatcg ggccctggcc aaggcggcca aggggctgtc cggcgtcacc ttccgcgatc
     1741 tgtacgagac ctatccggac ttcgcgatcg atatcgagac cgagcaggcc tcgctgctgg
     1801 cccatgacgt catcgccctg cagtttccgc tttactggta ctcgaccccg gccctgttga
     1861 aggagtggct ggatctggtg tggctgcacg gctttgccta tggccacgac ggcacggccc
     1921 tggtcggcaa gcgcctgttc gtggcctgca ccaccggggc cagcgccaag gcctatcacg
     1981 cccagggcta taaccgcttc acgatggacg acttcctgcg gccgctggag cagacggcgc
     2041 atctgtgcgg catggtctgg gagacgccgt ttgtcgtaca tggcgcggcg gtcaaggacg
     2101 acgaggccct gaaggccgag gccgaacgct acaaggcgcg gatcgcctcg ctgctgtcgg
     2161 ccacactgga ggtctgagcc atgcaatcct ttctgaccca gaccctgttc tatctcggcg
     2221 cggcggtgat ctcggtgccg atcgccaagc ggctggggct gggctcggtg ctgggctatc
     2281 tgatcgccgg cgtcatcatc gggccgcacg ccctgtcgct gatcggcgcc caggccgacg
     2341 tgatgcagtt cgccgaattt ggcgtggtca tcctgctgtt cctgatcggg ctggaggttc
     2401 agccctccac cctgtgggac atgcgcaagg cgatctttgg cctgggcggc gcccaggtgg
     2461 cgggcacggc gctgagcgtg gccggcgtct cgatgctgct gggcctgccc tggcagacgg
     2521 ccctggccgt gggtctggtg ctggccatgt cgtcgacggc tatcgtgctc cagaccctgg
     2581 atg
//
LOCUS       DAOMEO010000405         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4319_length_2571_cov_2.739269, whole genome shotgun sequence.
ACCESSION   DAOMEO010000405 DAOMEO010000000
VERSION     DAOMEO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4319_length_2571_cov_2.739269"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..2351)
                     /gene="parC"
                     /locus_tag="PLH31_09765"
     CDS             complement(75..2351)
                     /gene="parC"
                     /locus_tag="PLH31_09765"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HQR89530.1"
                     /translation="MNKPVLPPGGPGDGDRILDEPLTEALSRRYLAYALSTISSRALP
                     DVRDGLKPVHRRVLYAMNNMRLNPEAAARKCAKVVGEVMGNFHPHGDQSIYDALVRLA
                     QDFAQRIPLVEGQGNFGNIDGDNAAAMRYTECRMTTAATLLLDGIDEDAVDFRPTYDG
                     QDEEPVVLPSGFPNLLANGSSGIAVGMATSIPPHNAAELIDGCLLLLSRPEATTADLL
                     ERIPGPDFPTGGVIVEPRDSLLETYETGRGGVRVRAKWEKEDTGRGTYQIVVTEIPYQ
                     VKKSDLVEQLADLIESKKAALLGDVRDESAEDIRLVLEPKSKNVEPEVLMESLFKLSA
                     LESRFPVNMNVLDARGTPGVMGLKQALMAFLAHRREVLTRRAKHRLSKIEARLHILDG
                     LLIAYLNLDEVIRIVRYEDEPKQKLISTFALSDIQADAILNTRLRQLAKLEEMEIRRE
                     HAELMEERDGILAMLASDEKQWKLVGTGLREVRATLLKIRHPLDKPRINGITGRSIFA
                     DAPLVDAEAAIEAMIVREPITIILSDRGWIRAAKGKVEDPSELKFKEGDKLGFLVPAE
                     TTDKLLIFSSDGRFFTIGCDKLPSARGHGEPLRMMIELDDKVKILDVFPYKAGRKRIL
                     ASKLGYGFLMPEEEALANRKAGKQVLTVDAAGAAFCLEAVGDQLGVIGDNGKILIFPL
                     EELPEMPRGKGVKLQAYREGGLRDALSFNAETGGYWIDSAGRRRDWTDWTAFVGRRAS
                     AGKLAPKGFPTSKRFKPK"
BASE COUNT          409 a          841 c          854 g          467 t
ORIGIN      
        1 gccggtggcc gcagcggcgc agccaagcag gccggcgaca aatccgatcg ccttccgtct
       61 cagccgcccg ccgctcattt gggcttgaac cgtttcgagg tcgggaagcc cttgggcgcc
      121 agcttgccgg cgctggcccg gcggccgaca aaggcggtcc agtcggtcca gtcgcggcga
      181 cgcccggcgg agtcgatcca gtagccgccg gtctcggcgt tgaacgacag cgcgtcgcgc
      241 aggccgccct cgcgataggc ctgcagcttg acgcccttgc cgcgcggcat ttccggcagt
      301 tcttccagcg ggaagatcag gatcttgccg ttgtcgccga tcacgcccag ctggtcgccg
      361 acggcttcca ggcagaaggc cgcgcccgcc gcatcgacgg tcaggacctg cttgccggcc
      421 ttgcgattgg cgagcgcttc ttcctcgggc atcaggaagc catagcccag cttcgaggcc
      481 agaatgcgtt tgcggcccgc cttgtagggg aagacgtcga ggatcttcac cttgtcgtcc
      541 agctcgatca tcatccgcag cggctcgcca tggccgcgcg cgctgggcag cttgtcacag
      601 ccgatcgtga agaaccggcc gtcgctggag aagatcagca gcttgtcggt ggtctcggcc
      661 gggaccagga agcccagctt gtcgccttcc ttgaacttca gctcggacgg atcctccacc
      721 ttgcccttgg cggcgcggat ccagccgcga tccgacagga tgatggtgat cggttcacgg
      781 acgatcatcg cctcgatggc ggcctcggcg tcgaccagcg gcgcgtcggc gaagatcgag
      841 cggccggtga tgccgttgat acggggcttg tccagcgggt gcctgatctt cagcagggtg
      901 gcgcgcacct cgcgcaggcc tgtgcccacc agcttccact gcttctcgtc gctggccagc
      961 atggccagga tgccatcgcg ctcttccatc agctcggcgt gctcgcggcg gatctccatc
     1021 tcttccagct tggccaactg gcgcaggcgg gtgttgagga tggcgtcggc ctggatgtcc
     1081 gagagtgcga aggtcgagat cagcttctgc ttgggctcgt cctcgtaacg gacgatgcgg
     1141 atcacctcat ccagattgag gtaggcgatc agcaggccgt ccaggatgtg cagccgcgct
     1201 tcgatcttgg acaggcgatg cttggcccgg cgggtgagca cctcgcggcg gtgggccagg
     1261 aacgccatca gcgcctgctt cagccccatc acgcccggcg tgccgcgcgc gtccagcacg
     1321 ttcatgttga cggggaagcg gctttccagc gccgagagct tgaacaggct ctccatcagc
     1381 acttcgggtt cgacgttctt ggacttgggc tcaaggacga ggcgaatgtc ctcggcgctt
     1441 tcgtcgcgca cgtcgcccag cagagcggcc ttcttgctct cgatcaggtc ggccaactgc
     1501 tcgaccagat ccgacttctt tacctgatac ggaatctcgg tgacgacgat ctggtaggtc
     1561 ccgcgaccgg tgtcttcctt ctcccacttg gcccgaacgc gcacgccgcc gcggccggtc
     1621 tcgtaggttt caagcaagga gtcgcgcggc tcgacgataa cgccgccggt cgggaagtca
     1681 ggccctggga tgcgttccag caggtcggcc gtcgtcgcct cgggacggct gagcagcagc
     1741 agacagccgt cgatcagctc ggcggcgttg tgcggcggga tcgaggtcgc catgccgacc
     1801 gcgatgcccg acgagccgtt ggccaggagg ttgggaaagc ccgacggcag aaccaccggt
     1861 tcctcgtcct ggccgtcata ggtgggtcga aaatcgaccg cgtcctcgtc gatgccgtcc
     1921 agcagcagcg tcgccgccgt ggtcatccga cattcggtgt aacgcatggc cgcggcgtta
     1981 tcgccgtcga tattgccaaa attgccctgg ccctcgacca gcgggatgcg ctgggcgaag
     2041 tcctgggcca ggcgcaccag ggcgtcatag atcgactggt cgccgtgcgg gtggaagtta
     2101 cccatcacct cgcccaccac cttggcgcac ttgcgcgcgg cggcctcggg gttcaggcgc
     2161 atgttgttca tcgcgtacag cacgcgacgg tgaacaggct tcaagccatc gcgcacgtcc
     2221 ggcagggccc gcgaactgat cgtcgacagg gcataggcca gatagcggcg cgacagggct
     2281 tccgtcagcg gctcgtcaag gatgcggtcg ccgtcgccag gaccgccggg gggaaggaca
     2341 ggtttgttca tgaggaggaa tcgttaccgg gaccgcaccc tacggcatga tgattggtag
     2401 ttgaatcacc atttgaatcc gctctccccg gcgaaggccg gggtccagat cgaacccgga
     2461 gaggttggcg cgctcggctc aggcaaaggg cgcgtcatcc cccgactttc gggctgcatc
     2521 tgggccccgg ccttcgccgg ggagagcgga atatttggac gctaaaccct c
//
LOCUS       DAOMEO010000406         2567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4331_length_2567_cov_2.778264, whole genome shotgun sequence.
ACCESSION   DAOMEO010000406 DAOMEO010000000
VERSION     DAOMEO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4331_length_2567_cov_2.778264"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLH31_09770"
     CDS             <1..249
                     /locus_tag="PLH31_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQR89531.1"
                     /translation="KDFAQLADEKQLQTRSLPPVTTEDPKTSILSAPAPITRPPAAPA
                     VTAPATTAAPRPAAPRPAAPRPVAPAPAPTPAPAGDPQ"
     gene            246..761
                     /locus_tag="PLH31_09775"
     CDS             246..761
                     /locus_tag="PLH31_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89532.1"
                     /translation="MSMRGARPLHPARWLGIPLLVCIAATIVFSAPLRIWGLQLPEPV
                     FAMVPAFAWALIRPSILAPFAILVLGLFLDLFWGGPTGLWGLSLLVAYAMVLIARNMM
                     TGQSRPMMWAWFAGVTAVAMIAGFLFSMLDSLAMPSLLAVFWQFLATALLFPFAHRLV
                     DRFDDADVRFR"
     gene            766..>2567
                     /gene="mrdA"
                     /locus_tag="PLH31_09780"
     CDS             766..>2567
                     /gene="mrdA"
                     /locus_tag="PLH31_09780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQR89533.1"
                     /translation="MSEPSILFFEVNERQGVFHRRAFLMGGLAGAGLLALGGRLAQLQ
                     LVEAQRYQKLSAGNQFNYRLMPPPRGLILDRNGTPLASNRPNFRLMITKDKGMDVEAA
                     LDDLSKLVPIDGSRRARLIKDIARAPRKAPVAIMEDMSWEEFSRVNIRAPELPGVTAD
                     MGEVRVYPFGGAFAHVIGYVAKVSDRDLAAAEKDPTQDQDLLHHPGFRIGKQGVEKAF
                     DTDLRGRAGATKAEVDAQGRVVRLDPEGDIRPTPGKEIRLTLDADIQNRALEVMGEES
                     GAIVVMDCRTGDLLCMVSGPSFDANRFVKGLSGPEYKALAEYERKPLLDKAMTGTYPP
                     GSTFKPTVALAALSAGVDPEQRVFCGGGWYYGGRTWRCHKPGGHGSQNMNDAIKNSCD
                     IYFYQTALRIGPDAIANAARAMGFGQIFDIGIPGQKRGLVPDPAWKKRAFKRNPANQI
                     WFPGETPSYGIGQGALTVNALQLAVMTARLANAKKALLPRLIKSVGGVEQPSGASVPD
                     LPFSAEHLAYVRAGMAAVSAAGGTAFRGSQLGLGDIQMAGKTGTAQVRSFDKGVSRDS
                     KDVRWRLKDHNLFVAFAPYDDPRYAVSVIIEHGGS"
BASE COUNT          405 a          888 c          841 g          433 t
ORIGIN      
        1 aaggacttcg cccagctggc tgatgagaag cagcttcaga cccgcagcct gccgccggtc
       61 accaccgaag atcccaagac ctcgatcctc agcgcgcccg ccccgatcac gcggccgccc
      121 gccgcgccgg ccgtgaccgc tcctgcaacg actgcggcgc caagacccgc cgccccgagg
      181 cctgctgcac ccaggcccgt cgcgccagcg ccagcgccaa cgcccgcacc cgctggagac
      241 ccgcagtgag tatgcgcggc gcccggcctc tgcatccggc gcgctggctg ggcatccccc
      301 tgcttgtgtg catcgcggcg accatcgtgt tttcagcgcc cctgcgcatc tggggcctgc
      361 aactgcccga gcccgtattc gccatggtcc cggcctttgc ctgggccctg atccggccat
      421 cgatcctggc gcccttcgcc attctggtcc tggggctgtt cctcgacctg ttctggggcg
      481 gaccaacggg tctgtggggc ctgtcgttac tggtcgccta cgccatggtg ctgatcgctc
      541 gcaacatgat gacggggcag agccggccga tgatgtgggc ctggtttgct ggggtcacgg
      601 cggtggccat gatcgccggc ttcctgttct cgatgctcga cagcctggcc atgcccagcc
      661 ttctggcggt gttctggcag ttcctggcca cggccctgct gttcccgttt gctcaccgcc
      721 tcgtcgatcg cttcgacgac gccgacgtgc gtttccggta gggcgatgag cgagccctcc
      781 atcctctttt tcgaggtcaa tgaacgccag ggggtgttcc accgccgcgc gttcctgatg
      841 ggcggcctgg cgggcgcggg tctgctggcc ctgggcggac gcctggcgca gctgcaactg
      901 gtcgaggctc agcggtatca gaagctgtca gcgggcaacc agttcaacta ccgtctgatg
      961 cccccgccgc gcggcctgat cctggatcgc aacggcacgc ccctggcctc caaccggccc
     1021 aatttccggc tgatgatcac caaggacaag ggcatggatg tcgaggccgc cctcgacgac
     1081 ctgtccaagc tggtgccgat cgatggatcg cgccgggcgc gcctgatcaa ggacatcgcc
     1141 cgcgcgccgc gcaaggcgcc ggtggcgatc atggaagaca tgagctggga ggagttttcc
     1201 cgggtcaata tccgcgcgcc cgaactgccc ggcgtcaccg ccgacatggg cgaggtgcgg
     1261 gtctatccgt tcggcggcgc ctttgcccac gtcatcggct atgtcgccaa ggtctcggac
     1321 cgcgatctgg cggcggccga gaaggaccca acccaggacc aggacctgct gcaccatccc
     1381 ggcttccgca ttggcaagca gggcgtggag aaggccttcg acaccgacct gcgtggtcgc
     1441 gcgggcgcca ccaaggccga ggtcgatgcc cagggccggg tcgtgcgcct ggacccggaa
     1501 ggcgacatcc gtccgacacc aggcaaggaa atccgcctta ccctcgacgc cgacatccag
     1561 aaccgcgccc ttgaagtgat gggcgaggag agcggcgcca tcgtggtgat ggactgccgc
     1621 acgggcgacc tgctgtgcat ggtctcgggg cccagcttcg acgccaaccg tttcgtcaag
     1681 gggctcagcg gccccgagta caaggccctg gccgagtacg aacgcaagcc gctgctcgac
     1741 aaggcgatga ccggcaccta tccgccgggc tcgaccttca agccgaccgt ggcgctggcg
     1801 gccttgtcgg cgggtgtcga tcccgaacag cgcgtgttct gcggcggggg ctggtactat
     1861 ggcggtcgca cctggcgttg ccacaagccg ggcggccatg ggtcgcagaa catgaacgat
     1921 gcgatcaaga actcgtgcga catctatttc taccagaccg ctctacgaat cggacctgac
     1981 gccatcgcca atgcggcgcg ggccatgggc tttggtcaaa tcttcgacat cggcataccc
     2041 gggcaaaagc gcggtcttgt ccccgacccg gcgtggaaga agcgcgcctt caagcggaat
     2101 ccggcgaacc agatctggtt tccgggagaa accccgagct atgggatcgg gcagggggcc
     2161 ctgaccgtta acgcgctgca gctggcggtc atgacggcgc gcctggccaa tgcgaaaaag
     2221 gccctgcttc cgcgcctgat caagtcggtc ggcggcgtgg agcagcccag cggcgcgtcg
     2281 gtaccggacc tgcctttttc ggccgaacat ctggcctatg ttcgcgcagg catggcggca
     2341 gtgtcggccg ccggcggcac tgcctttcgt ggaagccagc tgggtctcgg cgacatccag
     2401 atggccggca agaccggcac cgcccaggtc cgcagtttcg acaagggcgt ctcgcgcgac
     2461 agcaaggacg tgcgctggcg gctgaaggac cacaatctgt tcgtcgcctt cgcgccctac
     2521 gacgatccgc gctacgcggt ctcggtgatc atcgagcacg gcggctc
//
LOCUS       DAOMEO010000407         2566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4336_length_2566_cov_2.458383, whole genome shotgun sequence.
ACCESSION   DAOMEO010000407 DAOMEO010000000
VERSION     DAOMEO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_2566_cov_2.458383"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..748)
                     /locus_tag="PLH31_09785"
                     /pseudo
     CDS             complement(47..748)
                     /locus_tag="PLH31_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
     gene            complement(880..>2566)
                     /locus_tag="PLH31_09790"
     CDS             complement(880..>2566)
                     /locus_tag="PLH31_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89534.1"
                     /translation="YSRNGGPGSATSLYIRGAETQHTVVLIDGVKLNDPSSTQGAYNS
                     GNLMIGDVSRIEVLRGAQSTLWGSQAIGGVVNMVTAEPKGTFESHASVEGGARDTAYV
                     RAGAGGAAERLTWRLAGSYYTTDGFSSYAPGSEKDGYQNTGLSGRVRFAFSDAVSAEV
                     RSVYSRGKNDFDGFNTDSAEFGRTEELVIYTGLNFALLDGRWSNRIAYGYTDTDRENI
                     NPARPAVPLTFDSTGKNKRWEYQGAFAVTETLTASFGAESERSTMGTRSPSVARPNPT
                     LKTGRVGVDSVYGQLQAEVAPGLTLTGGLRYEDHDTYGRHTLGQVSAAWALNQGNTVL
                     RASFGQGFRAPGLYELFSEYGNLDLSPEEFDSRDAGVEQRFWAGKAKVSATVFKREAD
                     NEIRFFSCSFGATDALCNPGGVFRFGYYKNIQKTRAQGLELVGQFDATDRLTLTGNYA
                     YTESENASGANAGRQLTRRPKNLANLSVAYRWPIGLNTIVAARHVGKTFNNETNTVRV
                     SSYTLVDLRASYPINDSLEVYGRVENAFDESYQTILNYGTPGRGAFIGIRAKF"
BASE COUNT          454 a          805 c          827 g          480 t
ORIGIN      
        1 cgtctgcgcc tgctgtatga agccaacccg atcggcatga tcatggagca ggccctgatc
       61 gcgccgaggc cagccagggc gccatgccaa gatccgccca gccggtgcgt cgcttgatct
      121 cgagacgacc atcgtcgaag agtgacgggt caccccggaa cttgttgatc aggaagccgc
      181 ggatcatggc gcgatcgtga tcgtcgagca caacatgcgc tccgaccagg gcggcgatca
      241 catggccccg atcaatgtcg ccgaccaaga tcaccggcac gtccgccgcg cgggcaaagc
      301 ccatattggc gatatcgccg gcccgaaggt tgatctcggc cggactgcca gcgccctcca
      361 cgatcacaag atcgcattcg gcctcgaggc gacgaaaact ctccaggacc gtgctcagta
      421 gcccggcttt ctgttcctgg tagtccctgg cctgccaggt tcccgccatc ttgcccagca
      481 ccaccagttg ggcgccgaca tcactctggg gcttgagcag aaccgggttc atattgactg
      541 tcggtggtgt gcgacaggcc atggcctgca gcgcctgtgc gcggccgatc tcgccgccat
      601 cggctgtgac cgcggcattg tttgacatgt tctgcggctt gaagggccga acactcaaac
      661 cgcgatttgt aaaaaggcgg cacaggcctg cgacgagcac agacttgccg acgtcggatc
      721 cacacccctg gatcatcaaa gcggccaaat acggcttcct ctggcgagcg gcgaccctgt
      781 caggtcaagt cgaacccagc gcgaacgcgg cggctgtctc gcggaagaca accgccgcgc
      841 cgcgcggctt cgttagagca acgagttcac ggctggtatc tagaacttgg cgcggatacc
      901 gataaatgcg ccgcgtccgg gcgtcccata gttcaggatg gtctgatagg actcgtcgaa
      961 ggcgttctcg acgcggccat agacctccag gctgtcattg atcgggtacg acgcccgcag
     1021 gtcgaccagc gtgtagctgc tgaccctgac cgtgttggtt tcgttattga acgtcttgcc
     1081 gacatggcga gcggcgacga tcgtgttcag gccgataggc cagcgatagg ccaccgacag
     1141 gttggcgagg ttcttgggac ggcgcgtcag ctgccggcca gcgttcgcgc ccgacgcgtt
     1201 ctccgactcc gtataggcgt agttgccggt cagggtgaga cgatctgtgg cgtcgaactg
     1261 gccgaccaac tccagaccct gggcccgggt cttctggatg ttcttgtagt agccgaagcg
     1321 gaagacgccg cccggattgc acaaggcgtc ggtcgcaccg aacgagcacg agaagaagcg
     1381 aatctcgttg tcggcctcgc gcttgaacac ggtcgccgaa accttcgcct tgccggccca
     1441 gaagcgttgc tcaacgccgg cgtccctgct gtcgaattct tcggggctca ggtcgagatt
     1501 gccgtactcg ctgaacagtt catacagacc cggcgcccgg aacccctggc cgaaactggc
     1561 ccggagcacg gtattgccct ggttcagcgc ccaggcggcc gagacctggc ccagggtgtg
     1621 gcggccatag gtgtcgtggt cctcgtagcg cagaccgccc gtgagagtca ggccgggtgc
     1681 gacctccgcc tgaagctggc catagacgct gtcgacgcca acgcgacccg ttttcagggt
     1741 ggggtttggt cgcgccacgc ttggcgatcg agtccccatc gttgagcgtt cgctttcagc
     1801 cccgaatgag gctgtcagcg tttcggtgac cgcgaaagcg ccctgatact cccagcgctt
     1861 gttcttgccg gtggagtcga aggtcagggg aacggcggga cgggccggat tgatgttctc
     1921 gcggtcggtg tcggtatagc cgtaggcgat gcggttgctc cagcggccat ccagcaaggc
     1981 gaagttcagg ccggtataga tcaccagttc ctcggtgcgg ccaaactcgg cggagtcggt
     2041 gttaaatccg tcgaagtcgt tcttgccgcg cgaatagacc gagcggacct cggccgagac
     2101 ggcgtcgctg aaggcgaagc gtacacgacc ggagagaccg gtgttctgat agccatcctt
     2161 ctcactgcct ggcgcataag atgagaagcc atcggtggtg tagtagctgc cggccagccg
     2221 ccaggtcagg cgttcggccg ccccgccggc gccggcccgg acgtaggctg tgtctcgggc
     2281 gccgccttcg acactggcat ggctttcaaa cgtgcctttt ggttcagcgg tgaccatgtt
     2341 gaccacgccg ccgatggcct ggctgcccca cagggtcgac tgggcgccgc gcagcacctc
     2401 gatgcgtgag acgtcaccga tcatcagatt gccggagttg tacgcgccct gggtcgagga
     2461 cggatcgttc agcttgacgc catctatcag tacgaccgtg tgctgggttt cggcgccccg
     2521 gatatagagg ctggtggccg agcctggccc gccgttgcgg ctgtaa
//
LOCUS       DAOMEO010000408         2565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4338_length_2565_cov_3.205976, whole genome shotgun sequence.
ACCESSION   DAOMEO010000408 DAOMEO010000000
VERSION     DAOMEO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4338_length_2565_cov_3.205976"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLH31_09795"
     CDS             complement(<1..1607)
                     /locus_tag="PLH31_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQR89535.1"
                     /translation="MLNRTTLDPETRVLYGDSLQPPPGYVFDAAVATTFSLDFETALA
                     APVSLALFAAENRDDILAHPLALLEGAERIAGRLVVFADAGHLHAQGRPHSRLCSLLE
                     RIIVEVAAPRGGAFHPKMWALRYRPTRAEDPTLLRLLVLSRNLTRDRSWDISLRLDGE
                     LTRRPDAGNRPLFDLLSRLPDLAVAGITNEARELTAEIAQDMRRARWTTPERFDEVAF
                     ALNGFGGSIWQPPRCARLGVISPFCDTDALDLLAGLPTAEKPILISRPDQLACIEAET
                     LDAFERVSVLDEMAASEDGEEVSASALQGLHAKAFIAEIGWDTVLTIGSGNATRPALL
                     SGNNVELFASLKGKRSRVGSIEQIMGEKGFGRLTRTFVLSELEPVDPAEISAEKRLDE
                     ARRALCRGALRLRCERVADDDAAGHPWRVWLTPSESLPLKGVGALTVWPITRGDGHAC
                     DVLSALRSGEAVDVGAMPMVDLTRFLAFRLVEETEKASALFSTGLVMDGLPAERHAAI
                     LRWAIDSRDAFFRYLRLLLSELGEPFSA"
     gene            complement(1597..2466)
                     /locus_tag="PLH31_09800"
     CDS             complement(1597..2466)
                     /locus_tag="PLH31_09800"
                     /inference="COORDINATES: protein motif:HMM:NF039739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HQR89536.1"
                     /translation="MGRDAGAAVQRLPSSVYWAGLSTWGVRLFPGSIDGLFSSLRHLQ
                     RSRPRVRESEDAASETQALHIWTPSLPARPADLLEAVTFRLAPEEAQFIIDQLVAKQP
                     RSLLTFLARTGSGAADCHYIWEHPHLADFPDDTRALVAHGELFSSVMHGASLLYNLLL
                     SELRAKPEWVETYKARLRDWRASLDMPAIRAWSLDAFWQTIDHPAHRIRPAAKRFVTE
                     WLELVRAGEVGSGHSAAGRIVQERERRLKTSQSRFANRTVRDRWTGASGVDRLSFRWA
                     QARSHLRDLADAE"
BASE COUNT          462 a          824 c          886 g          393 t
ORIGIN      
        1 gcgctgaagg gctcgccaag ttcggaaagc agcagccgca ggtaacggaa gaaggcgtca
       61 cggctatcga tcgcccagcg caggatcgcg gcgtgtcgct cggcgggaag tccatccatg
      121 accaggccag tgctgaacaa tgcggaggcc ttctcggtct cctcgaccag ccgaaaggcg
      181 aggaagcgag tcagatcgac catcggcatc gcgcccacat cgacggcctc gccggagcga
      241 agcgccgaca ggacgtcaca ggcatggcca tcgccacggg tgatgggcca gacggtcagg
      301 gcgcccacgc ccttgagcgg cagagactca gatggcgtca accagacccg ccagggatgc
      361 cccgcagcgt cgtcgtccgc gacccgctcg cagcgcagcc gcagcgcgcc gcgacacagc
      421 gcgcggcgtg cctcatcgag ccgcttttcc gcagaaattt cggccgggtc gaccggctcc
      481 agctcactga gaacgaaggt ccgggtcaga cgcccgaagc ccttctcgcc catgatctgc
      541 tcgatgctgc cgacgcgtga tcgtttgccc ttgagcgacg cgaacagctc gacattgttg
      601 cccgagagca aggccggccg cgtcgcattg ccggagccga tcgtcagaac ggtatcccag
      661 ccgatctcgg cgatgaaggc cttggcatgg agcccctgca acgcactggc ggagacctct
      721 tcgccgtctt cgctcgcggc catctcgtcc agaacggaaa cgcgttcgaa cgcgtcaagc
      781 gtctcggcct cgatgcaagc cagttgatcg ggtcggctga tgaggatcgg cttctcggcg
      841 gttggcaggc cagccagaag atcgagcgcg tcggtatcgc agaatgggga aatgacgccg
      901 agacgtgcgc accggggcgg ctgccagatg ctgccgccga acccgttgag ggcgaaggcc
      961 acctcgtcaa agcgctcggg cgtcgtccac ctggcgcggc gcatgtcctg tgcgatctct
     1021 gcggtgagtt cgcgtgcctc gttggtgatt ccggccaccg ccaggtcggg aaggcggcta
     1081 agcaggtcga acagcggccg attgccagcg tcgggccggc gcgtcagctc gccgtcgagg
     1141 cggagggaga tgtcccagga gcggtcgcgc gtaaggtttc tcgacaggac gagaagtcgc
     1201 agcaaggtcg gatcctcggc ccgggttggc cgataccgca gggcccacat cttcgggtga
     1261 aaggcgccgc cgcgaggcgc ggcgacctcg acgatgatcc gttcgagcag cgagcaaagt
     1321 cgcgaatgcg gtcggccttg ggcatggaga tgcccggcgt cggcaaacac caccagccgc
     1381 ccggcgatcc gttcggcgcc ctcgagcagg gccaggggat gggcgaggat gtcgtcgcgg
     1441 ttctcggccg cgaacaaggc caggctgaca ggcgccgcca gcgcagtctc gaaatcgagc
     1501 gagaaggtcg tcgccaccgc ggcgtcgaag acgtagccgg gcgggggctg gaggctgtcg
     1561 ccgtagagca cgcgggtttc gggatcgagg gtggtcctat tcagcatcgg ctagatcccg
     1621 caggtgcgat cgggcctgcg cccatcggaa cgagaggcga tcgacacctg acgcgcccgt
     1681 ccaccggtct cgcacggtcc ggttagcgaa gcgcgactgg ctggttttca gccgccgctc
     1741 gcgctcctgc acgatccgcc cggcggcgct gtggccgcta ccaacctcac cggcacgaac
     1801 caactccaac cattcagtga cgaaacgctt ggccgcaggc cgaatgcggt gggccgggtg
     1861 gtcgatggtc tgccaaaagg catcgagcga ccaggcgcgg atcgcaggca tgtccaggct
     1921 cgcccgccag tcccgcagcc gcgctttgta ggtttcgacc cactccggct tggcccgcag
     1981 ctcgctgagc agcagattgt agaggagcga ggcgccgtgc atcacgcttg aaaacagctc
     2041 gccatgcgcg accaacgccc gcgtatcgtc ggggaaatct gcgagatgcg ggtgttccca
     2101 gatgtagtgg cagtcggccg cgccgctccc ggtgcgggcg agaaacgtca aaaggctgcg
     2161 gggctgtttc gccacaagct gatcgatgat gaactgggcc tcttcgggcg cgaggcgaaa
     2221 ggtcaccgcc tccagcaagt ccgctggtct ggccggcagc gagggcgtcc agatgtgaag
     2281 ggcctgcgtt tccgacgcgg cgtcttcgct ttcgcggacc cgcggtcggc tgcgttgaag
     2341 atggcgcagc gaggagaaga ggccgtcgat cgagcccgga aagaggcgga caccccaagt
     2401 cgacagccct gcccaataga ccgagctggg gagccgctgc accgccgcac cggcgtcgcg
     2461 accgatgatg ccgttcgatt ctccgccggc tttgagcgca ttggcgagcc gtgatgtttg
     2521 ggtacacgca gaaagtcgcg tatattagca ggtttagcgg acctg
//
LOCUS       DAOMEO010000409         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4344_length_2561_cov_2.633679, whole genome shotgun sequence.
ACCESSION   DAOMEO010000409 DAOMEO010000000
VERSION     DAOMEO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4344_length_2561_cov_2.633679"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLH31_09805"
     CDS             complement(<1..731)
                     /locus_tag="PLH31_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR89537.1"
                     /translation="MSRFVEGVDRRQPTLLPECLDDYVAAENPVRVVDVFVDELDLGG
                     LGFEVKPAATGRPAYHPAMLLKLYVYGYLNKVQSSRRLEREARRNVELMWLTGRLAPD
                     HKTIADFRKDNGPAIQAACAQFVVLCRQIGLFGAALVAIDGSKFKAVNTRDKNFTPYK
                     LKKRLEQVAEHIAGYLRDLDTADRQEGDTIEARSDRLKEKVERLREQMRMLQAMDAQV
                     AASADGQVSLTDPDARSMATSGRGSG"
     gene            1028..1333
                     /locus_tag="PLH31_09810"
     CDS             1028..1333
                     /locus_tag="PLH31_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HQR89538.1"
                     /translation="MEKRRPTYDLEAIKTAFGSVETLAMTSTALRGAAALGFDRAGVV
                     ETIAGIERQMFFKSMTTFADHRVWQDVYHVPARGMTLYVKFQADVVTEFTVMSFKEK"
     gene            1336..>1607
                     /locus_tag="PLH31_09815"
     CDS             1336..>1607
                     /locus_tag="PLH31_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89539.1"
                     /translation="MAVSKTDLPETMVSPETGETLRRGVRPFIVAYKGKSTTVELPGY
                     YPEHSDDGVHVGDDMAVTDAALRSLKEEIEGIPSPATIRRVREKLKL"
     assembly_gap    1608..1617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1866..2287)
                     /locus_tag="PLH31_09820"
                     /pseudo
     CDS             complement(1866..2287)
                     /locus_tag="PLH31_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505079.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
BASE COUNT          443 a          826 c          791 g          491 t
ORIGIN      
        1 ccgctgccgc gtccgctggt ggccatcgag cgggcatcgg gatcggtcag cgagacctga
       61 ccgtcggccg aggccgccac ttgcgcgtcc atcgcctgca gcatccgcat ctgctcgcgc
      121 agacgctcaa ccttctcctt cagccggtcg gacctggcct cgatggtatc cccctcctgc
      181 cggtcggcgg tgtccagatc gcgcagatag ccggcgatgt gctcggccac ctgctccagg
      241 cgcttcttca gcttgtaggg cgtgaagttc ttgtcgcgcg tgttgaccgc cttgaacttg
      301 ctgccgtcga tggccaccag cgccgcgccg aacaggccga tctggcggca cagaaccacg
      361 aactgggcgc aggccgcctg gatcgctggg ccgttgtcct tgcgaaagtc agcgatggtt
      421 ttgtggtcgg gcgccagccg gcccgtcagc cacattagct cgacattgcg ccgcgcctcg
      481 cgctccagcc gccggctgga ctgcacctta ttcaggtagc cgtacacgta gagcttcagc
      541 agcatcgccg ggtgataggc cggtcgcccc gtcgccgccg gcttcacctc aaagcccagc
      601 ccgcccaggt ccagctcatc gacgaataca tcgactaccc gcaccgggtt ctcggcggcg
      661 acatagtcgt ccaggcattc gggaagcagc gtcggctgtc ggcgatcaac accttcaacg
      721 aagcggctca tgcacacctc ccgactgctc gtcaggagtc tagctgatcc gtcgagtttt
      781 cacacggcct cggtcacatc cggccaccgg cgcgattgct aacctgtcta cccaatagat
      841 acacggtccg tgcggggcca aagtcttgcg ctggacgctc gggccagggt tgcaatgacc
      901 gcaattgagc ttcagcggtg ccggtctggc cggtctacga gcgggctcat tgcttttcct
      961 ccattcctta tttttgcgct ggaaggccca ggttgaatca ccaccataat ggtggcatat
     1021 tggtgctatg gagaagaggc gtccgaccta cgatctcgaa gcgatcaaga cagcgttcgg
     1081 atcggttgag acgctcgcaa tgacatcaac ggccctgcgc ggcgcagccg ccctgggctt
     1141 tgatcgcgca ggcgtcgtcg agaccatcgc gggcatcgaa cgtcagatgt tcttcaaatc
     1201 gatgacgacc ttcgccgacc accgcgtctg gcaggacgtc tatcacgtcc cagcacgtgg
     1261 aatgacgctc tatgtgaagt tccaggccga cgtggtgacc gaattcacgg tcatgtcgtt
     1321 caaggagaaa tgaccatggc ggtatcgaag acggacctgc ccgaaacgat ggtctctccc
     1381 gagaccggag agacgctgcg ccgtggcgtc cgtccgttca tcgtggccta taagggcaag
     1441 agcaccacgg tcgagctgcc cggctattat ccggagcata gtgacgacgg cgtccatgtc
     1501 ggtgacgata tggcggtcac cgatgcggcg ctgcgctcgc tgaaggagga gatcgaaggc
     1561 attccctccc cggccacgat caggagggtg cgcgagaagc tgaagctnnn nnnnnnncgc
     1621 agcgcgaggc gggtggtctc ctcaaggttg gggagaatgc tttcgataag tatgagcgag
     1681 gcttggtcga gccgagcggt ccgaccagcc agttgttgcg tctgctcgac caccaccccg
     1741 agttggtcga agaattgcgc gagaccgcag cgtaggcggc gatcgaggtc tcatcggctg
     1801 agcggacgtc ggcggaagca ccccatgctt catgcacttc gcagcctcct gctgctcgcc
     1861 gctacgatcg atactccggc cgcgttgtag gtgaaggcga agaacaggtt ctggcggatg
     1921 ttgcccgtgg tcgcccggct gagttgacgt gcgcgcacga tcccctggag gtcgcgcttc
     1981 aggagtgtca ccccgcgctc tcgatcgcga catccgtgcc cgtgcccatg gcgatgccta
     2041 cgtcggcggc ggccaacgcg ggggcgtcgt tcacgcggtc gcccgccatg gcaacgatcc
     2101 tgctctcctt ccgcaacctc gcgaccacct tcgccttgtc ctcgggcagg atctcggcct
     2161 cgacctcctc gatgccgagc ttgcgggcga cagcctgggc ggtcgtcctg ttgtcgccgg
     2221 tcagcatgac gacgcggatg ccggcgccgc ggaacgccgc gacggcggcg ggcgtcgtcg
     2281 ccttgatgga ggcgccatca ctacgacagc cgtggccaga tcgaacgcca cctcgaaggc
     2341 cttcgtctgc gcctataatt tcggccggca cctactacca gcgggccggc cgaagcatac
     2401 ggatatgcgc tggccgcccg ccagctcgat gagaaatctg cccacggatc cgtcgtagtt
     2461 gcggatggta tcccagagat gatagccttt cctcggagca ccctgtcgca ttttttgtcg
     2521 gttgtctcgc agatctggat agagtagatt atccacagaa a
//
LOCUS       DAOMEO010000410         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4349_length_2559_cov_2.140575, whole genome shotgun sequence.
ACCESSION   DAOMEO010000410 DAOMEO010000000
VERSION     DAOMEO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4349_length_2559_cov_2.140575"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2429)
                     /locus_tag="PLH31_09825"
     CDS             complement(<1..2429)
                     /locus_tag="PLH31_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR89540.1"
                     /translation="MRPIKMRLTAFGPFPDTQTVDFRPALGARLFGIYGPTGAGKTSI
                     LDGICFALFGESSGQERQGDDLRSHHATPDIETEVSLIFEVGAKRYNVVRRPRQTVRG
                     KRGDALVERQHWAALYDATDIEVDDIEADNPGVVMEERKVEVVADRMRSILNYSAAQF
                     RQVVLLPQGQFRQLLTASSDQRSTVLRGLFDVSLYERFVERLKAEASELRNEVETGRS
                     AINGLLQAHAVSDTDALAALIETLTADVGVQTAGRDAARGVRDTARATLQAAQQIQDR
                     FTEHDAALDGLNGVLARAPEIDTLNLRKATAERAVACVAADDRANEADNDLTAGVAAR
                     TLAADQASESARLLSEAMAGLQASAARQPVRDAAIAAVTQLEGVQKRVAGAEPLREAA
                     RSSSQAALEAKAALKLAADDHDVAEQANGAANAELTSVQQTVLRISQIEGALQLLRQA
                     REEAAQFANATGAGEKFAAARSAASANHERLAGVLAACRVAESTAEEALASAQAAHLA
                     AKLEDGAPCPVCGATEHPLPAGGVGEGLGLDAAWRQARSAREVADADERQAAQVAARA
                     DGEWTQAVSTLAALKAPERDVTAFTADIVAADAELEALQAGPDIATVQAAVDAAKLHL
                     ASTTSALAAARDQHVAADKAATSAAAALAASLADVPEDLRDAAAVTLRVHAAIEHRDQ
                     LNDAHQLAVENERRASEAAQAARSGLGHAEARVKELTAARDTQRASFVAAKATAGLSD
                     TAYTAAKADIPNIQNLASTITEHVAGLAAAQDRKDRAVAAIAGLDRPNLEASSAALTD
                     ADAILTKAEELL"
BASE COUNT          393 a          879 c          828 g          459 t
ORIGIN      
        1 aggagttcct cagccttggt tagaatcgcg tcggcgtccg tcagcgcggc cgagctggcc
       61 tccaagttgg ggcgatccag accagcaatg gccgcaacgg cgcggtcctt ccgatcttgc
      121 gcggcggcca agccagcgac gtgctcggtg atcgtcgagg ctaggttctg gatgttgggg
      181 atatctgctt tggccgccgt gtaagcagta tcactcagcc cagctgtcgc ctttgcggcc
      241 acgaacgaag cccgctgcgt atcgcgcgcc gcggtcagct ccttcacccg agcctcagcg
      301 tggccgagcc cagagcgtgc ggcctgggcg gcctcactag cgcgccgctc gttctcgacc
      361 gccaattggt gcgcgtcgtt caactggtcc cggtgttcga tagccgcatg gacgcgaagg
      421 gtgaccgccg cagcgtcgcg cagatcttcg ggcacgtccg cgagcgacgc cgccagcgcg
      481 gctgccgccg aagtcgccgc cttatcagct gcgacatgct gatcgcgagc ggcggcgagc
      541 gctgaggttg tcgaggccag gtgcagtttg gcggcatcga cggcggcctg gaccgtggca
      601 atgtccgggc ccgcctgcag tgcttcgagt tctgcatccg cggcgacgat gtctgccgtg
      661 aacgcagtga catcccgctc aggcgccttc agcgccgcca acgtagagac ggcctgcgtc
      721 cattcgccgt cagccctcgc cgcgacctgc gcggcctgac gttcgtccgc gtccgcgact
      781 tctcgcgcgc tgcgcgcttg gcgccatgcg gcgtcgaggc cgagaccctc gccgacccca
      841 ccggcaggca gagggtgctc cgtggctcca cagaccggac atggcgcccc atcctcgagt
      901 tttgctgcca agtgcgcagc ctgcgccgaa gcaagtgctt cttcggcagt agattcggcc
      961 accctgcagg ccgcgaggac gccggccagt cgttcatggt tggccgaagc ggccgacctc
     1021 gcggcggcga acttctcgcc agcgccggtg gcgttcgcga actgagcggc ctcttcccgc
     1081 gcctgtctca agagctgcag agcgccctca atctgcgaga tccgcagcac agtctgctga
     1141 acgctagtga gctccgcgtt agccgcgccg ttcgcctgct cggcgacatc atggtcatcg
     1201 gccgccagct tgagagcggc cttcgcctct agagcggctt gcgacgagct gcgcgccgcc
     1261 tctcgaagcg gttcggcgcc ggccacacgc ttctgcacac cctcgagttg ggtcaccgcc
     1321 gcgatagcgg cgtcccgtac gggttgacgg gcagcggagg cttgcaggcc tgccatcgcc
     1381 tcagaaagca ggcgcgccga ctcactcgcc tggtctgccg cgagagtccg ggctgccacg
     1441 ccggcggtta gatcattgtc ggcctcattg gcccgatcat cggcggcgac acaggccaca
     1501 gcgcgctcag cggtcgcttt gcgcaggttg agggtgtcaa tctcgggcgc gcgggcgagg
     1561 accccattaa ggccgtccag cgccgcgtcg tgttctgtga atcgatcctg gatctgctga
     1621 gccgcttgca gcgttgcgcg ggccgtatcc cggaccccgc gcgcagcgtc gcggcccgcc
     1681 gtctgtacgc cgacatcggc cgttagggtt tcgatcaggg ccgccagcgc atcggtgtcg
     1741 gacacggcat gggcttgcag aagcccgttg atcgccgacc tgcccgtctc aacttcatta
     1801 cgcagctcag aggcttcggc cttcagccgt tcaacaaagc gctcgtagag ggagacgtcg
     1861 aacaaccccc gcagcacagt ggagcgctgg tcgctggagg ccgtcagcag ctggcggaat
     1921 tgtccctggg gtaacagcac cacctgacga aactgggcgg cgctatagtt caagatcgac
     1981 cgcatccgat cggccacgac ctcgaccttg cgttcctcca tcacgacgcc ggggttgtcg
     2041 gcttcgatgt catcgacctc aatgtcggtc gcgtcgtaga gggcggccca gtgctggcgc
     2101 tcgacaaggg catcgccccg tttgcctcgg accgtctgac gcggtcgccg cacgacatta
     2161 tatcgcttgg ccccgacctc gaagatcagg ctcacctcgg tctcaatatc gggcgtggca
     2221 tggtgtgaac gcaggtcgtc gccttgccgc tcctggcctg acgattctcc gaagagtgcg
     2281 aagcaaatgc catccaggat cgaggtcttg cccgctcctg ttggtccgta gatgccgaac
     2341 aggcgagcgc ccaacgccgg gcggaaatcg acggtctgcg tgtcggggaa cggtccaaaa
     2401 gccgtcaggc gcattttgat cgggcgcatg tcagacctcc tcgctctgga ggtcggtcag
     2461 ggcctcgtcg atcatgcccg tctcgacgtc gtcaggcccc ctcccgcgca ccgcatcgag
     2521 aaagccggcc accagcttca ccgggtcttg ccgatcaac
//
LOCUS       DAOMEO010000411         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4351_length_2559_cov_1.628594, whole genome shotgun sequence.
ACCESSION   DAOMEO010000411 DAOMEO010000000
VERSION     DAOMEO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4351_length_2559_cov_1.628594"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="PLH31_09830"
     CDS             <1..108
                     /locus_tag="PLH31_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQR89541.1"
                     /translation="YGVSRERVRQIEVRAFEKLQKTMREAAIAKNMVEA"
     gene            248..958
                     /locus_tag="PLH31_09835"
     CDS             248..958
                     /locus_tag="PLH31_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR89542.1"
                     /translation="MPLPQLILLPGLLNDAELWRDQISGLADVARPYVPDLTRGETIR
                     ALAEAVLAQAEPTFALAGFSMGGYVAQAIARIAPQRIERLALLDTSIRVDSPERAARR
                     RALTEAARKPGAFIGISHTILKSYVDASRLDDAELTGRIVDMTQRLGREVFIRQNSLE
                     REDGEAALKALRCPLVIICGENDAITPAKDHREMARTIGCSHLVVIPRAGHMTPMEAP
                     GMVNGALRHWLERPSGTR"
     gene            1112..1417
                     /locus_tag="PLH31_09840"
     CDS             1112..1417
                     /locus_tag="PLH31_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89543.1"
                     /translation="MKKTMLFALAAFAPLAIASAASAQDAPIATADTPVTASEVVVQA
                     QIAYRNRSEQTAPVLEYGLDYFPALRTPDRRRRPQARAQRHLPVGRAGVGRRPSARP"
     gene            1353..>2559
                     /locus_tag="PLH31_09845"
     CDS             1353..>2559
                     /locus_tag="PLH31_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR89544.1"
                     /translation="MPSVTFLSDVLESDGARLRGLDAAYTQILINGEKVPGAGADRSF
                     FVGRIPAEMIERIEIVRSASANRSGDAMAGALNIVLRDAYALEGGFLRAGAIMLNDGR
                     IRETLGAVWGGQVGPGRLLLGANIQGRRNPKEKMSWRYDEPGAPLDNTELQKDTRNGT
                     DYSFNANYRVPLAGGELDLSGFYVRTDRFADEDSLEYEGGRERTADLSTFNDNDVDIQ
                     TDNWAINGKFKREMLGGETAVKLGYAVFTDSQYEFENEIEYLRDAIPFPDGDRFTADK
                     ARTELEDKEFTAKLEHKRDLGGVNLEVGVQYQKKDRDALITEIPRNRYNLPAATPPAD
                     RAFAPVLGGDTAIAESRIDPYVMLSGKAGDVKWEAGLRYETTDVEILDNTAAPALRQF
                     STDYAILLPS"
BASE COUNT          469 a          911 c          800 g          379 t
ORIGIN      
        1 tacggcgtca gccgcgaacg ggttcgccag atcgaggtcc gggctttcga aaagctgcaa
       61 aagaccatgc gcgaggcggc catcgccaag aacatggtcg aggcttaagg ctgtagtggc
      121 tgctccccct ctgggggagc tgtcggcaaa gccgactgag ggggcaaatg cggcttaggc
      181 cgagctagcc ccctccggcc ctctgggcca cctcccccaa gaggggagga gctattatag
      241 ggtcgctatg cccctccccc agctcatcct gctgcctggc ctgctcaacg acgccgagtt
      301 gtggcgcgat cagatcagcg gcctcgccga tgtcgcccgc ccctacgtcc ccgacctgac
      361 ccgaggggag accatccgcg cactggccga ggcggtgctg gcgcaggccg aacccacctt
      421 cgccctcgcc ggcttttcga tgggcggcta tgtggcccag gcgatcgccc ggatcgcgcc
      481 gcagcgtatc gagcgcctag ccctgctgga cacctcgatc cgggtcgaca gtcccgagcg
      541 cgcggcgcgg cggcgggctc tgaccgaagc ggcccgcaag ccgggcgcgt tcatcggcat
      601 cagccacacg atcctcaaaa gctatgtcga cgcctcacgg ctcgatgacg ccgagctcac
      661 cggccgcatc gtcgacatga cccagcgcct gggccgcgag gtgttcatcc gccagaacag
      721 cctggagcgc gaggacggcg aggcggcgct gaaggccctg cgctgcccgc tggtgatcat
      781 ctgtggcgaa aatgacgcta tcacccccgc caaagatcac cgcgagatgg cccggaccat
      841 cggctgctcg cacctcgtgg ttatccccag ggctggtcat atgaccccga tggaggcccc
      901 cggcatggtc aacggggcct tacgccactg gctggagcga ccgtcaggga cacgatagtc
      961 cgtaaacgga tctcctcttt cgacacaaat ttgagatgaa tccgtcaggg aaacatcact
     1021 ggcggccgat cccgcgcgaa actatgccgt gcccgggaaa acacgcgcgc acccctcaat
     1081 aaacgtgggc gcgctggatg gggatccttc gatgaaaaag accatgttgt tcgccctggc
     1141 ggcgttcgcg ccgctggcta tcgcctcggc cgcctcggcc caggacgcgc cgatcgcgac
     1201 cgccgacacg ccggtcaccg cttcggaagt cgtcgtccag gcccagatcg cctatcgcaa
     1261 ccgctccgag cagaccgccc cggtgctgga gtacggcctg gactactttc cagcgcttcg
     1321 aacccctgac cgccggcgac gccctcaagc gcgtgcccag cgtcaccttc ctgtcggacg
     1381 tgctggagtc ggacggcgcc cgtctgcgcg gccttgacgc ggcctacacc cagatcctga
     1441 tcaacggcga gaaggtgccc ggcgccggcg ccgaccgttc gttcttcgtc ggccgcatcc
     1501 ccgccgagat gatcgagcgc atcgagatcg tgcgcagcgc ctcggccaac cgcagcggcg
     1561 acgccatggc cggcgccctg aacatcgtgc tgcgcgacgc ctacgccctt gagggcggct
     1621 tcctgcgggc cggcgccatc atgctgaacg atggccgcat tcgcgagacc ctcggcgcgg
     1681 tgtggggcgg ccaggtcggc cccggccgcc tgctgctcgg cgccaacatc cagggccgtc
     1741 gcaaccccaa ggaaaagatg agctggcgct atgatgagcc cggcgcgccg ctcgacaaca
     1801 ccgaactgca aaaggacacc cgcaacggca ccgactattc gttcaatgcc aactatcgcg
     1861 tgcccctggc tggcggcgag ctggacctga gcggcttcta tgtccgcacc gaccgcttcg
     1921 cggacgagga ctcgctggaa tatgaaggcg gccgcgagcg caccgccgac ctgtcgacct
     1981 tcaacgacaa cgacgtcgac atccagaccg acaactgggc gatcaacggc aagttcaagc
     2041 gcgagatgct gggcggcgaa acggccgtga agctgggcta tgcggtgttc accgacagcc
     2101 agtacgagtt cgagaacgaa atcgaatatc tgcgcgacgc catcccgttc ccggacggcg
     2161 accgcttcac cgccgacaag gcccgcaccg agctggaaga caaggaattc accgccaagc
     2221 tcgagcacaa gcgcgacctg ggcggcgtga acctggaagt cggcgtccag taccagaaga
     2281 aggatcgcga cgcgctgatc accgagatcc cgcgcaatcg ctacaacctg cccgccgcga
     2341 ccccgccggc cgatcgcgcc ttcgcgccgg ttctgggtgg tgacaccgcc atcgccgaaa
     2401 gccgcatcga cccctacgtg atgctgtcgg gcaaggccgg cgacgtgaag tgggaagccg
     2461 gtctgcgcta cgagaccacc gacgtcgaga tcctcgacaa caccgccgcg ccggcgctgc
     2521 gccagttctc gaccgactac gccatcctgc tgccctcgg
//
LOCUS       DAOMEO010000412         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4368_length_2553_cov_2.305044, whole genome shotgun sequence.
ACCESSION   DAOMEO010000412 DAOMEO010000000
VERSION     DAOMEO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4368_length_2553_cov_2.305044"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..916)
                     /locus_tag="PLH31_09850"
     CDS             complement(326..916)
                     /locus_tag="PLH31_09850"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQR89545.1"
                     /translation="MIELQRCTTWKGMAGDPVYEAYHDTEWGVPEYDSRALWEKLVLD
                     GFQAGLSWITILRKRDAFRAAFADFDPEIVARFGEADRARLMADAGIIRSNAKIDAAI
                     SGARIYLDMRERGEDLSDMLWGMVGGAPVQSAWTSETGTPARTPLGDEMAKALKAKGF
                     KFCGPVIVYAFMQATGLVNDHLTTCYRHEECQAMGR"
     gene            complement(972..1796)
                     /locus_tag="PLH31_09855"
     CDS             complement(972..1796)
                     /locus_tag="PLH31_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HQR89546.1"
                     /translation="MTSPAPVPLSQARLPSRAVIALSGPDWRSFLQGLITQDVETLTP
                     GELRLGAILTPQGRLLYDLFIAGTQDGALLDVAAQHREAIILRLTMYRLRAKVEIAPS
                     DLTVTAIFPADEALKADEGFYRDPRLPQLGARAYGLAAEPTTDEDVYDAHRLALGVPG
                     PADWLTDKTYPIEANFDLLNGIDFKKGCFVGQETTSRMKRRGTIKTRMAPITFDGPPP
                     AFGAEILAGELRAGEVLSGRDGRAMAALRLDRIAAGGLTVDGRPVSVDWPGWMPTT"
     gene            1907..>2553
                     /locus_tag="PLH31_09860"
     CDS             1907..>2553
                     /locus_tag="PLH31_09860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQR89547.1"
                     /translation="MTQRAFPILFITATRIGDAVLSSGLIRMLADEIPGARFTIVAGP
                     LAAPLFAHVPGLDRVIVMEKGKGKGHWFKLWNQVRHRKWSLIVDLRGSATALFLRREK
                     RAIWKKVPGEVVHKVIDAARVLKLEGAPPAPHLYITPEVQALADQMLATKPGEVAGPL
                     LAMGPAANWVGKVWPIERFAQTAGQLLGPDGPLAGGRLLILGGPEDTRMVEELRMA"
BASE COUNT          404 a          828 c          895 g          426 t
ORIGIN      
        1 agattatagg cccagtctgt cgtgaggccc gcatgggtct cgccgcaaat gctacagacg
       61 aaatgctcgc tcataggctc gggcccttga tccgctcggc gctcttttgt ccccgactct
      121 cggcgaaatg cctaggtctc acttcaatcc tcagccgaca cctcaagtct aggttctagc
      181 gggtgtgacg agcggctaga cacgcgcgcc ctgagagcat cgaagtagcg ggcgtcgccc
      241 ttcgcgcctg gaggagagac ggcgccgtca agcaacagat cgcgcaggcg ctggagcgcg
      301 gcatccttgg caaaaattga caagactacc gccccatcgc ctggcactcc tcatgccggt
      361 aacacgtcgt caggtgatca ttcaccagtc ccgtcgcctg catgaacgcg tagacgatga
      421 ctggcccgca gaacttgaag cccttggcct tcagcgcctt ggccatctca tcgcctaggg
      481 gcgtgcgggc gggggtgccg gtctcggagg tccaggcgct ctgcaccggg gcgccgccga
      541 ccatgcccca cagcatgtcg ctgaggtctt cgccgcgctc gcgcatgtcg agatagatcc
      601 gcgcgccgct gatggccgcg tcgatcttgg cgttggagcg gatgatgccg gcgtccgcca
      661 ttagccgggc gcggtctgcc tcgccaaatc gcgcgacgat ctcggggtcg aagtcggcga
      721 aggcggcgcg gaaggcgtcg cgtttgcgca ggatggtgat ccacgacagg ccggcctgga
      781 agccgtccaa gaccagcttt tcccacaggg cgcgggaatc gtactccggc acgccccatt
      841 cggtgtcgtg ataggcctca tagacggggt cgcccgccat gcccttccag gtggtgcagc
      901 gttggagctc gatcatgcgg tcgagcttgg cgcaaccgcg cggcgatttc cagaactttt
      961 cgtgaacagg ctcaggtcgt gggcatccag ccgggccagt cgacgctgac ggggcgtccg
     1021 tcgacggtga ggccgcccgc cgcgatgcgg tccagccgca gggcggccat ggcccggccg
     1081 tcgcggcctg acagcacctc gccagcccga agctcgcccg ccaggatctc ggcgccgaag
     1141 gcgggcggcg ggccgtcgaa ggtgatcggg gccatgcggg tcttgatcgt accgcgccgc
     1201 ttcatgcggc tggtggtttc ctggccgacg aaacagcctt tcttgaagtc gatgccgttc
     1261 agcaggtcga aattggcctc gatcggatag gtcttgtcgg tcagccagtc ggcgggacct
     1321 ggcacgccca gggccaaacg gtgggcgtca tagacgtctt catccgtggt cggctcggcc
     1381 gccaggccgt aggcgcgggc gcccaactgc ggcaggcgcg ggtcgcggta gaagccttca
     1441 tcggccttaa gggcttcgtc ggcggggaag atcgcggtga cagtcaggtc gctgggggcg
     1501 atctcgacct tggcccgcag ccgatacatc gtcaggcgca ggatgatcgc ctcgcggtgc
     1561 tgggcggcca cgtccagcag ggcgccgtcc tgcgttccgg cgatgaacag gtcgtagagc
     1621 agccggccct gcggggtgag gatcgcgccc agccgcagct cgccgggggt cagggtctcg
     1681 acatcctggg tgatcaggcc ctgcaggaag gatcgccagt cgggaccgct gagggcgatc
     1741 acggcgcggg atgggaggcg ggcttgggaa agcgggacgg gggcggggct ggtcatcccg
     1801 cagatgtggc gccgcagcga cgctggcgcc acccctcgtc tcaatcggaa agctgagccg
     1861 ttatagcggg atgcgccgac tcagatggcg gcctatagat gatccgatga cacagcgggc
     1921 cttcccgata cttttcatca ccgccacgcg gatcggcgac gccgtgctgt cgtccggcct
     1981 gatccgaatg ctggccgacg agatcccggg cgcgcgcttc accatcgtgg ccggacccct
     2041 ggccgccccg ctgttcgccc atgtgcccgg tctcgaccgg gtgatcgtca tggagaaggg
     2101 caagggcaag ggccactggt tcaagctgtg gaaccaggtg cgccacagga agtggagcct
     2161 gatcgtcgac ctgcgcggct cggccacggc cctgtttctg cgtcgcgaga agcgggccat
     2221 ctggaagaag gtccccggcg aggtcgtcca caaggtgatc gacgcggccc gggtgctgaa
     2281 gctggaaggc gcgccgccgg ccccgcatct ctacatcacg cctgaggtcc aggccctggc
     2341 cgaccagatg ctggcgacca agccgggcga ggtcgcgggt cccctgctgg ccatgggtcc
     2401 cgccgccaac tgggtgggca aggtctggcc gatcgagcgc ttcgcccaga cggccgggca
     2461 gctcttgggt cccgatggcc cgctggccgg cggtcgcctg ctgatcctgg gcggacccga
     2521 ggacacccgc atggtcgagg aattgcgtat ggc
//
LOCUS       DAOMEO010000413         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4373_length_2551_cov_2.370192, whole genome shotgun sequence.
ACCESSION   DAOMEO010000413 DAOMEO010000000
VERSION     DAOMEO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4373_length_2551_cov_2.370192"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLH31_09865"
     CDS             complement(<1..1401)
                     /locus_tag="PLH31_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQR89548.1"
                     /translation="MILPASLAMAQTPAQAPAPKPAKPAEAATAVEGVTVTSDATAMR
                     TSIDRRSYSVANDLSARTGSISDALRNIPSVEVDVQGNVSLRGDPNVTIMIDGKPSGM
                     FNGDGKADALQQLPADQIDRVEVMTNPSAAFRPDGSAGVINLITKKTSKPGTTGSIRA
                     NIGPDGRYNGGISGSRRAGKLTLSGDAGYRYDNQQFSQTIDRAMLDTASQTFVESRQT
                     ATFKNKGGGLNLRGGLDYDVDKQNRLSTEIRYRGFSFDTEGVETYEGENLAGVVDRAF
                     RRATTADMSRENVAVSGDWRRQLKGAEHELTAHVEYEVTDFSRDSRAFADNTPGVDEY
                     EAYGFGSDQTRANFKLDYTKPLPGDSKLKAGVDLEAVRNDYDNYGARGLTPDGLIVDP
                     NRTNRFLYDQDVYAAYLTYEKPFGDFTVQGGLRLEQVEIKTDQVTANLKGANDYFRAY
                     PSLHMGYKLSEAQTLSA"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1607..2002)
                     /locus_tag="PLH31_09870"
     CDS             complement(1607..2002)
                     /locus_tag="PLH31_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR89549.1"
                     /translation="MRLLLDTHVALWTLTDDPRLTEDARTLILDPANEVCVSAVTLWE
                     IAIKHGLGRAGAGAMPVSAHEAQVLFGAAGYLLIPITPEQAVIVETLPNIHADPFDRL
                     LVAQGLTEALVLVTHDAVVASYNTRIIKI"
     gene            complement(1999..2247)
                     /locus_tag="PLH31_09875"
     CDS             complement(1999..2247)
                     /locus_tag="PLH31_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQR89550.1"
                     /translation="MLIVNMHDAKSQLSKLVDAVESGETDEVVIARNGRPAARLVPMT
                     LARRTPVRIGIAKGLFAAPADPNAQDAEVAALFLDAKD"
     assembly_gap    2281..2290
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2438..>2551
                     /locus_tag="PLH31_09880"
     CDS             2438..>2551
                     /locus_tag="PLH31_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89551.1"
                     /translation="MASVSSAGPSAGMSAEGGKVNMAFVAAIVAVATIGGFM"
BASE COUNT          426 a          786 c          845 g          474 t
ORIGIN      
        1 ggcgctcagc gtctgggctt cgctgagctt gtagcccatg tgcaggctgg gataggcgcg
       61 gaaatagtcg ttcgcgccct tcagattggc cgtgacctga tcggtcttga tctccacctg
      121 ctccaggcgc aatccgccct gcaccgtgaa atcgccgaac ggcttctcat aggtcagata
      181 ggccgcatag acgtcctggt catagaggaa gcggttggtg cggttggggt cgacgatcag
      241 gccatcgggc gtcaggccgc gcgcgccgta attgtcatag tcgttgcgaa ccgcctccag
      301 atcgacaccg gccttcagct tggagtcgcc cggcagaggc ttggtgtagt cgagcttgaa
      361 attggcgcgg gtctggtcgg agccgaagcc ataggcctcg tactcatcga cgcccggcgt
      421 gttgtcggcg aaggcgcggg agtcgcgcga gaagtcggtg acctcgtatt cgacatgggc
      481 ggtcagctcg tgctcggcgc ccttcagctg ccggcgccag tcgccgctga ccgcgacgtt
      541 ttcgcggctc atatcggccg tggtggcgcg gcggaaggcg cgatcgacca caccagccag
      601 gttttcgccc tcataggtct cgacgccttc ggtgtcgaag ctgaagccgc gatagcggat
      661 ctcggtgctg aggcggttct gcttgtcgac atcgtagtcg agaccgcccc gcaggttcag
      721 cccgccgccc ttgttcttga acgtcgcggt ctggcggctt tccacgaagg tctggctggc
      781 cgtgtcgagc atggcccggt cgatggtctg cgagaactgc tggttgtcgt agcgatagcc
      841 ggcgtcgccg gacagggtca gcttgccggc gcggcgcgaa ccgctgatcc cgccgttgta
      901 acggccatcc gggccgatgt tggcgcggat cgagccggtg gttccgggct tgctggtctt
      961 cttggtgatc aggttgatca ccccggccga gccatccggt cggaaagcgg ctgaaggatt
     1021 ggtcatgacc tcgacgcggt cgatctggtc ggcgggcagc tgctgcaggg cgtcggcctt
     1081 gccgtcgccg ttgaacatgc cagacggctt gccgtcgatc atgatcgtga cgttgggatc
     1141 gccgcgcagg ctgacattgc cctgcacatc cacctcgacc gaagggatgt tgcgcagggc
     1201 gtcgctgatc gagccggtcc gcgccgacag gtcgttggcg acgctgtagc tgcggcgatc
     1261 gatcgaggtg cgcatggcgg tggcgtcgct ggtcaccgtc acaccctcaa ccgccgttgc
     1321 ggcctcggcc ggcttggcgg gcttgggtgc gggagcctgg gccggcgtct gggccatggc
     1381 caggctggcc ggcaggatca tcgcggtcgc cgcgagcagg agtttaaagc gcaagacgca
     1441 tnnnnnnnnn ngaccgccgg acgcgcaagc aactacacat gtaaaataca tgatcgcttt
     1501 tgctgtagag cccggcgcga caccgggatc aaatgtcttt tcagcaatgt tttgcttggc
     1561 gccgggcctg cgttttccgc gcaacggtcg cccagaccgg tgcgggtcag atcttgatga
     1621 tccgcgtatt gtaggacgcc accacggcgt cgtgcgtcac cagcaccagg gcttcggtca
     1681 gaccctgggc gaccagcaga cggtcgaacg gatcggcatg gatgttcggc agggtttcga
     1741 cgatcaccgc ctgctcaggc gtgatcggga tcagcaaata gccggccgcg ccgaacagca
     1801 cctgggcttc gtgagcggag accggcatgg cgcccgcacc cgcgcgaccc aggccatgct
     1861 tgatggcgat ctcccacaag gtcacggcgc tgacgcagac ttcgttggcc ggatcgagga
     1921 tcaatgttcg cgcgtcttcc gtcagacgcg gatcgtcggt cagggtccaa agcgcgacgt
     1981 gggtgtcgag cagcagcctc aatccttggc atccagaaac agcgccgcga cttcggcgtc
     2041 ctgggcgttc ggatcagccg gagcggcgaa caaccccttg gcgatgccga tccgcacggg
     2101 cgtccggcgc gccaaggtca tcggcaccaa tcgcgcggca ggccggccat tgcgcgcgat
     2161 caccacctcg tcggtctcgc cactctcaac ggcatccacc aacttcgaaa gttgcgactt
     2221 ggcgtcgtgc atgttgacta taagcatgac cagactcgct attagctaat tctagctaac
     2281 nnnnnnnnnn aatgcgctgg gccggaagac gaccaaacaa ccgccacgtc tcgccgcttg
     2341 cgcgctgata ccggtaacac taaggtcgcc gcaacgagcc gtccagcaag gacgctcagg
     2401 gattaaacgc ggccttcggg ccatggggag gaaaccaatg gcttcagtat ccagcgccgg
     2461 acccagcgcg ggcatgagcg ccgagggcgg caaggtcaac atggccttcg tcgcggcgat
     2521 cgtggccgtc gccacgatcg gcggcttcat g
//
LOCUS       DAOMEO010000414         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4377_length_2549_cov_1.836808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000414 DAOMEO010000000
VERSION     DAOMEO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_2549_cov_1.836808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..925)
                     /locus_tag="PLH31_09885"
     CDS             complement(32..925)
                     /locus_tag="PLH31_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQR89552.1"
                     /translation="MSIFRIAHLSDPHLPPPPGAFGWRDIVSKRLLSRIAWRRKQKEH
                     QPWVLAALIADIKAYAPDHIAITGDLTNFASAVEIEAARQWLSGLGATDSVTLSPGNH
                     DALVGARTPDRFAVWSAWLGDGDDIAFPRLRIRDGVAIINLCSAIPTAPHLATGRLGD
                     AQLARLEALLADPAHRDLFRVVLIHHPPLQGAVSRRKSLEDLDALRAILTRQSADLVL
                     HGHAHESLVAALPGPSGLAIPVLGVPSASALGKGNHPAARWHAIEITRTGSAPVSVRV
                     VARGLDPETGQPAELGRYVLA"
     assembly_gap    1115..1124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1125..2088
                     /locus_tag="PLH31_09890"
     CDS             <1125..2088
                     /locus_tag="PLH31_09890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQR89553.1"
                     /translation="PAIGDVVVVDAGWVFDEALIRALAGRPGVALVDETGRIVAAHLP
                     SSAGEDLSAIAADVPRLTAMELGSAYNSELRKREPPVLERLTPETVDAVEKRLFKGSY
                     KGVTDLVTKYVWPAPARVVTRWCALAKLTPNMVTFASFLMVLAAFWLFWTGQYGLGLV
                     CAWIMTFLDTVDGKLARVTLTSSKWGNVFDHGIDLIHPPFWWWAWFVGLGALGFAVPH
                     GGLLLAIVVGGYVAQRVEEGIFLSLFKVEMHAWRPFDSFFRLITARRNPNLLFLTAST
                     AVGRPDVGFVLVAAWTAICFVVHASQIVQGLLTPRGGVTSWLSR"
     gene            2088..>2549
                     /locus_tag="PLH31_09895"
     CDS             2088..>2549
                     /locus_tag="PLH31_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQR89554.1"
                     /translation="MKVGVVHNPRSHANMGRSADSWAPDDVMLVRPATREALEVDLVA
                     FAARGVELLVIDGGDGTIRDVLSLLPRAFGDAPPLIALLPSGKTNVLAIDLGVPPGRT
                     LQEALIAARSPRAVVKHRAPLEVAWDDGRPTMCGFVFGLGAFVRATNMSKSV"
BASE COUNT          382 a          815 c          889 g          453 t
ORIGIN      
        1 cgctctcttc ggcgaagcgg gccttgcagg cctaggccag aacgtagcgc cccagctccg
       61 caggctggcc cgtttccgga tccaggcccc gtgcgaccac gcggacagaa acaggagccg
      121 acccggtgcg cgtgatttcg atcgcgtgcc agcgcgccgc aggatggttg cccttaccga
      181 gcgccgaggc cgagggaacg cccagcactg ggatcgccaa gccgcttggg ccaggcaggg
      241 ccgcaacgag actctcgtgg gcatggccat gcagcaccag atcggcgctc tggcgggtca
      301 ggatcgcgcg cagggcgtcc aggtcctcca gggacttgcg cctggagacg gcgccctgga
      361 gcggcggatg atggatcagg acaacgcgaa agaggtcacg atgggccgga tcagcgagca
      421 gggcctccag tcgggccagt tgcgcgtcgc ccagccggcc ggtggcgagg tgcggcgcgg
      481 tgggaatggc ggaacacagg ttgatgatcg ccacgccgtc gcggatccgg agcctgggaa
      541 aagcgatgtc gtcgccgtcg cccagccagg cggaccacac cgcgaagcga tcgggagttc
      601 tggcgcccac gagcgcgtcg tggttgccgg ggctgagagt gacgctatct gtagcgccaa
      661 gtccgctcag ccactggcgt gcggcctcga tctcgacagc cgacgcgaaa ttggtcaggt
      721 cgcccgtgat ggcgatatgg tcgggcgcat aggccttgat atcggcgatg agggccgcca
      781 gcacccaggg ctgatgttcc ttttgcttgc ggcgccacgc aatgcggctc agcaggcgct
      841 tggaaacgat atcccgccag ccaaaagcgc ccgggggcgg cggcagatgc gggtcggaaa
      901 ggtgggcaat gcggaatatc gacaagtcag aatcgcgctt tttcgccata aacgaggcaa
      961 agctgcttat cactcgccac gggtttccag gggttctcgc acattgaccg gcggaagtaa
     1021 tcaaggcgcc tgcgccaagc ctgtggccgt aacagttggc caggcctcta cgcgcatctg
     1081 gggtatgacc tcggctcagc ggctgtcgcg gatcnnnnnn nnnngcctgc catcggcgac
     1141 gtggtcgtcg tcgacgccgg ctgggtgttc gatgaggcgc tgatccgcgc cctggccggg
     1201 cggcccggcg tggccctggt ggacgagacc ggacgcatcg tggccgctca cctgccgtct
     1261 agcgcgggcg aggacctttc cgccatcgcc gctgacgttc cgcgcctgac ggccatggag
     1321 ctgggctcgg cctataacag cgaactgcgc aagcgcgagc cgccggtgct ggagcgcttg
     1381 acgccggaaa cggtcgacgc ggtcgagaag cgattgttca agggctccta caagggcgtc
     1441 accgatcttg tgaccaagta tgtctggccc gcgccggccc gggttgtcac ccgctggtgc
     1501 gccctggcca agctgacgcc caacatggtg accttcgcca gcttcctgat ggtgctggcc
     1561 gcgttctggc tgttctggac gggccaatat ggcttgggcc tggtctgcgc ctggatcatg
     1621 accttcctcg acaccgtgga tggcaagctg gcccgggtga ccctgacctc gtccaaatgg
     1681 ggcaatgtct tcgaccacgg tatcgacctg atccatccgc ccttctggtg gtgggcctgg
     1741 ttcgtcggcc tgggcgcgct gggcttcgcc gtgccgcatg gcggactgct gctggccatt
     1801 gtggtgggcg gctatgtggc ccagcgggtg gaggagggga tctttctctc ccttttcaag
     1861 gtcgagatgc atgcctggcg gccgttcgac agtttcttcc ggctgatcac cgcgcgccgc
     1921 aatcccaacc tgctgttctt gaccgcctcg accgcagttg gccgtccaga cgtggggttt
     1981 gtccttgtgg cggcctggac cgcgatctgc ttcgtcgttc acgccagcca gatcgttcag
     2041 ggcctgctga cgccccgcgg cggcgtcacg tcctggctgt cgcgctgatg aaggtcggcg
     2101 tcgtccacaa tccgcgcagc cacgccaata tggggcgtag cgccgactcg tgggcgcccg
     2161 acgacgtgat gctggtgcgg ccggccacgc gcgaggcgct ggaggtcgat ctggtggcct
     2221 tcgccgcccg gggcgtggag ttgctggtca tcgacggcgg cgacggcacg atccgcgatg
     2281 tgctgagcct gctgccgcgc gcctttggcg atgcgccgcc gctgatcgct ctgttgccgt
     2341 ctggcaagac caatgtcctg gcgatcgacc ttggcgttcc gccaggccga accctgcagg
     2401 aagctctgat tgcggcccgc tcgcccaggg ccgtggtcaa gcaccgggcg ccgctggagg
     2461 tcgcctggga tgatggccgc ccgacgatgt gcggctttgt attcggcctg ggcgcctttg
     2521 tgcgcgccac caacatgtcc aagagcgtc
//
LOCUS       DAOMEO010000415         2545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4384_length_2545_cov_2.069076, whole genome shotgun sequence.
ACCESSION   DAOMEO010000415 DAOMEO010000000
VERSION     DAOMEO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_2545_cov_2.069076"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLH31_09900"
     CDS             <1..404
                     /locus_tag="PLH31_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQR89555.1"
                     /translation="LRVRRQADTALALANVIAEHRKTKAVLYPFRPDHPGHNVAKSQM
                     SGGGTVVALDLGSREAAFKFLNALEIVDISNNLGDAKSMATHPPTTTHRSVPEAERPS
                     LGVTEGGVRLSVGLESLDDLTRDVTRALDQA"
     gene            385..849
                     /gene="apaG"
                     /locus_tag="PLH31_09905"
     CDS             385..849
                     /gene="apaG"
                     /locus_tag="PLH31_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQR89556.1"
                     /translation="MRSTRRETSPPLQRIRRRRGADGGIYEARTRDIVVRVAPVYLPE
                     ESAPEEGLYLWSYTVEIENHGVETVHLVARRWAITDAFNRCSEVEGSGVVGEQPELKP
                     REAFRYVSSCPLPTPSGAMRGHYQMMTEGGEVFEAEIPEFSLHLPGAARRYN"
     gene            complement(846..1754)
                     /locus_tag="PLH31_09910"
     CDS             complement(846..1754)
                     /locus_tag="PLH31_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HQR89557.1"
                     /translation="MTDTTAIPARDDIVSAFQIEGLPVRGRVVRLGAAVDEVLTRHDY
                     PEPVANLLGEACALAALVGSSLKFEGRLIVQAQGDGPVRYVVVDYDTTGGLRGYCRFD
                     PEEVAQISEGFARPGAKTLLGGGVFIMTLDQGADMDRYQGVTPIEGETLALCAEQYFK
                     QSEQTPTRVRLAVGQADTGEGLKWRAGGILIQSIAGDETRGETLEAWNHAQAFFETTG
                     EDELIDPSLGTPTLLWRLFNEDGVRLIEDKPLRSFCRCSDDRIAVVIQSFTEAEKAEM
                     IEPDGKIHVTCEYCSRVYAVEPDAVA"
     gene            complement(1927..>2545)
                     /locus_tag="PLH31_09915"
     CDS             complement(1927..>2545)
                     /locus_tag="PLH31_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQR89558.1"
                     /translation="VMIRANSHADVERFAQVSTVPVINGLTDKSHPCQIMADLLTIEE
                     HRGDIGGKTIAWVGDGNNVCSSFIHAAPLLGFKLKIACPAVYHPDMVDLARAADLQGQ
                     VTMTDDPREAVRGADVVVADTWVSMGDTDHDQRLAAFEPYQVDDALMDLSAPDGVFLH
                     CLPAHRGEEVTDAVLDGPRSLAWDEAENRIHAQKSVLAWAFGAIG"
BASE COUNT          413 a          860 c          863 g          409 t
ORIGIN      
        1 acctgcgcgt ccgccgtcag gccgacaccg ccctggccct ggccaatgtg atcgccgagc
       61 acaggaagac caaagcggtg ctctatccct tccgccccga ccatccgggc cacaacgtcg
      121 ccaagtcgca gatgagcggc ggcggcacgg tggtggcgct ggacctgggc agccgagaag
      181 cggcgttcaa gttcctgaac gcactggaga ttgtcgacat ctccaacaat ctgggcgacg
      241 ccaagtcgat ggccacccac ccgccgacca ccacccaccg ctcggtgcct gaagccgaac
      301 gcccgtcgct gggcgtcacc gaaggcggcg tgcgcctgtc ggtcggcctg gaaagcctgg
      361 acgatctgac gcgagatgtg acccgtgcgc tcgaccaggc gtgaaacctc gccgccgctg
      421 cagcggatcc gtcggcgtcg cggcgccgat ggcggcatct atgaagcccg cacgcgcgac
      481 atcgtcgtgc gcgtggcgcc cgtgtacctg cccgaggaat ccgcccctga ggagggcctc
      541 tatctgtggt cctacacggt ggaaatcgag aaccatggcg tcgagaccgt gcatctggtc
      601 gcccgtcgct gggccatcac cgacgccttc aaccgctgca gcgaagtcga gggttccggc
      661 gtcgttggcg aacagcccga gctgaagcct cgcgaggcgt tccgctacgt ttccagctgc
      721 cccctgccaa cgccctcggg cgccatgcgc ggccactatc agatgatgac cgagggcggc
      781 gaggtgttcg aggccgagat ccccgagttc tcgctacatc tgccgggggc ggcgcggcgg
      841 tataattagg cgaccgcgtc cggctccacc gcatagaccc gcgagcagta ttcgcacgtg
      901 acgtgaatct tgccgtcggg ctcgatcatc tcggccttct cggcctcggt gaacgactgg
      961 atgaccaccg cgatgcggtc gtccgagcag cggcagaacg agcgcaacgg cttgtcctcg
     1021 atcaggcgca cgccgtcctc gttgaacagc cgccacagca gggtcggcgt gcccaacgac
     1081 gggtcgatca gttcgtcctc gccggtggtc tcaaagaagg cctgggcgtg gttccaggcc
     1141 tccagcgtct cgccgcgcgt ctcgtcgccg gcgatcgact ggatcaggat gccgcccgcg
     1201 cgccatttca ggccttcgcc ggtgtcagcc tgaccgacag ccaggcgcac ccgggtcggg
     1261 gtctgttccg actgcttgaa atactgctcg gcgcacaggg ccagggtttc gccctcgatc
     1321 ggggtgacgc cctgatagcg gtccatgtcg gcgccctgat ccagggtcat gatgaagacg
     1381 ccgccgccca gcagggtctt ggcgcccgga cgggcgaagc cttcggaaat ctgggccact
     1441 tcttcgggat cgaaccggca atagccgcgc aggccgccgg tggtgtcgta gtcgaccacc
     1501 acataacgca ccgggccgtc gccctgagcc tggacgatca gccggccctc gaatttcagg
     1561 ctggagccaa ccagggccgc cagggcgcag gcctcgccca gcaggttggc gacaggttcg
     1621 ggatagtcgt gacgggtcag cacctcgtcg acggcggcgc ccaggcgcac gacgcggccg
     1681 cgcacgggca ggccttcgat ctgaaaggcg gaaacgatgt cgtcgcgggc gggaatggcg
     1741 gtggtgtcgg tcatggggcg gttaaatagg gggtgcaggc gggaattgcg agggcgtcag
     1801 ccagatcctc cccttttggg ggaggtggcc cggagggccg gagggggcta gctggatgct
     1861 ggccgcgctg ccccctcagt cggctttgcc gacagctccc ccagaggggg agcatctaac
     1921 aggtcactac ccgatcgccc cgaacgccca ggccagcacc gacttctgcg cgtggatgcg
     1981 gttttccgcc tcgtcccagg ccagcgagcg cgggccgtcc agcaccgcgt cggtgacttc
     2041 ctcgccccgg tgggccggca ggcagtgcag gaagacgccg tcgggcgccg acaggtccat
     2101 cagggcgtcg tcgacctgat agggctcgaa cgcggccaga cgctggtcat gatcggtgtc
     2161 gcccatcgag acccaggtgt cggccaccac cacgtcggcg ccgcgcacgg cctcccgcgg
     2221 atcatcggtc atcgtcacct ggccttgcag gtcggcggcg cgcgccaggt ccaccatgtc
     2281 ggggtgatag accgccggac aggcgatctt cagcttgaaa cccagcagcg gcgcggcgtg
     2341 gatgaagctg gagcagacat tgttgccgtc accgacccag gcgatggtct tgccgccgat
     2401 atcaccgcga tgctcttcga tggtcagcag gtcggccatg atctggcagg ggtggctctt
     2461 gtcggtcagg ccgttgatca ccggcaccgt cgagacctgg gcgaagcgtt ccacgtcggc
     2521 gtggctgttg gcgcggatca tcacc
//
LOCUS       DAOMEO010000416         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4403_length_2535_cov_2.533468, whole genome shotgun sequence.
ACCESSION   DAOMEO010000416 DAOMEO010000000
VERSION     DAOMEO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4403_length_2535_cov_2.533468"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLH31_09920"
     CDS             complement(<1..171)
                     /locus_tag="PLH31_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR89559.1"
                     /translation="MNTMIAPVMALVAWSLVIWLWMYLRRIPAMLKAGIKPQDAAFPG
                     SLDVLPAGPRQAA"
     gene            complement(260..1108)
                     /locus_tag="PLH31_09925"
     CDS             complement(260..1108)
                     /locus_tag="PLH31_09925"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQR89560.1"
                     /translation="MSILKKIAFQGEPGANSHEACRTYFPDYEAYPCATFEEAFEAIK
                     TGVCALGMIPIENSIAGRVADVHHLLPGAGLKIIGERFKPIRFQLMVNKGVRLEDVKV
                     VASMPIALSQCRNSLRALGVTTESAGDTAGAAKALALKPDPTRGAVAPALAAEIYGLE
                     ILARDIEDERHNTTRFLVMTADANPTPPPFTHRCVTSFVFKVRNLPAALYKALGGFAT
                     NGVNMTKLESYMEGGAFTATFFYAEVDGRPEDRSLALAFDELKFFSDKFEILGVYPAD
                     PFRDRT"
     gene            complement(1105..1842)
                     /locus_tag="PLH31_09930"
     CDS             complement(1105..1842)
                     /locus_tag="PLH31_09930"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQR89561.1"
                     /translation="MNPIVLIPARMAATRLPGKPLADIGGLPMIVRVLNQALKAKVGP
                     VAVAAGDREIVEAVRAAGGVAVLTDPELPSGSDRILAALAQLDPGFEHDVVINLQGDM
                     PFVDPAVLSACAGVLEDFGDCDIATVVAAEASPADRTNPDVVKAVLAMEDDGKSGRAL
                     YFTRSTLYGDGPVWRHIGLYGYRREALEAFNAAAPSALEKREKLEQLRAMELGMTIRA
                     AVALEAPISVDNPSDLEAARIQAGKTQ"
     gene            2044..2236
                     /locus_tag="PLH31_09935"
                     /pseudo
     CDS             2044..2236
                     /locus_tag="PLH31_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518403.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
     assembly_gap    2237..2246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2247..>2535
                     /locus_tag="PLH31_09940"
     CDS             <2247..>2535
                     /locus_tag="PLH31_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HQR89562.1"
                     /translation="EFGAKNPVWDYDHLDGFLKAPGKYVSGTNMTFVGIKKPEDRVAL
                     IAYLHSLGSTLPVPAPKPAEAAAPAAAEGAPATAEEAGAAAAGAPVSPSEAP"
BASE COUNT          428 a          823 c          870 g          404 t
ORIGIN      
        1 ggccgcctgg cgaggtccgg ccggcagcac gtccagcgag cccggaaagg ccgcgtcctg
       61 cggcttgatc cccgccttca gcatcgccgg aatgcggcgc agatacatcc acagccagat
      121 caccagcgac caggccacta gcgccataac cggcgcgatc atcgtattca ttggagcgtc
      181 cctccccgga cgttttgatt gtgcggggag gttagacgaa gaacgccgtt attgcgaagc
      241 ttgcccctgg ggcagcatct caggtccggt cgcgaaaagg atcggccgga tagacgccca
      301 ggatctcgaa cttgtccgag aagaacttca gctcatcaaa ggccagggcc aacgaccgat
      361 cctccggccg gccatcgacc tcggcgtaga agaaggtcgc cgtgaaggcg ccgccctcca
      421 tgtagctttc cagcttggtc atgttgacgc cgttggtcgc gaagccgccc agggccttgt
      481 acagcgccgc cggcaggttc ctgaccttga acacgaagct ggtcacgcag cggtgggtga
      541 acggcggcgg ggtgggattg gcgtcagccg tcatcaccag gaagcgggtg gtattgtggc
      601 gctcgtcctc gatgtcgcgg gccagaatct caaggccata gatctcggcg gccagggccg
      661 gagccacggc gccgcgggtc ggatcaggct tcagggccag ggccttggcg gcgccggcag
      721 tgtcgcccgc cgactcggtg gttacgccca gggcgcgcag gctgttgcgg cactggctga
      781 gtgcgatggg catcgaggcg acgaccttca cgtcctccag cctgacgccc ttgttgacca
      841 tcagctggaa gcggatcggc ttgaagcgct cgccgatgat cttcaggccc gcgcccggca
      901 gcaggtggtg gacatcggcg acgcggccgg cgatcgagtt ctcgatcgga atcatgccca
      961 gggcgcagac gccggtcttg atggcctcga acgcctcctc gaaggtggcg cagggatagg
     1021 cctcatagtc cggaaaatag gtgcggcagg cctcgtggct gttggcgccg ggttcgccct
     1081 gaaaggcgat cttcttgagg atgctcattg ggtcttccca gcctgaatac gggcggcttc
     1141 gagatcggag ggattgtcga cggaaatcgg ggcttccagc gccacggcgg cgcggatggt
     1201 catgcccagc tccatggccc gcagctgttc cagcttctcg cgcttttcca gcgccgaggg
     1261 cgcggcggcg ttgaaagcct ccagggcctc gcggcgatag ccatagaggc cgatgtggcg
     1321 ccagacgggg ccgtcgccat agagggtgga gcgggtgaaa tagagggcgc ggccgctctt
     1381 gccgtcgtcc tccatggcca gcacggcctt gaccacgtcg ggattggtcc ggtcggccgg
     1441 cgaggcctcg gcggccacca ccgtggcgat gtcgcagtca ccgaagtcct ccagcacgcc
     1501 agcgcaggcc gaaagcaccg cggggtcgac gaagggcata tcgccctgca ggttgatcac
     1561 cacgtcgtgc tcgaaacccg gatcgagttg cgccagcgcg gccaggatgc ggtcggaccc
     1621 cgagggcagc tcggggtcgg taagcaccgc cacgccgccg gccgcgcgca cggcctcgac
     1681 gatctcgcga tcgcccgccg ccacggccac cgggcccacc ttggccttga gcgcctggtt
     1741 cagaacgcgg acaatcatcg gcaagccgcc gatatcggcc agcggtttgc ctggcaggcg
     1801 ggtcgcggcc atccgcgccg ggatcaggac gatggggttc atggtcgcaa atcgctcctg
     1861 cccggttgcg cgaggggcgg tagcgtgtaa gagacgcgag cgcaacaaaa ggcgcgcgcg
     1921 agagattcct cgccgtggaa tttggccccg ccccaggcgg gtcggtaaga ggctgacaag
     1981 gctgagatga gcgacctgac gttcaacaag attgctggcg gcgtgctgct gaccggcctg
     2041 gttatttttg gtctgcgtga agtgtcgacc atcgtgttcg acaagcctgc gcccgaaaag
     2101 gctggctacg agatcgcggt ggaagagtcg accgagggcg gcgccgccgc tgtcgaggtc
     2161 gcgcccgact ggggcaccgc cctgctgctg ccggccaata tcgccgcggg ccaagctgtc
     2221 gccgccaagt gcgccgnnnn nnnnnnagga atttggcgcc aagaacccgg tctgggacta
     2281 cgaccacctg gacggcttcc tgaaagcgcc gggcaagtat gtctcgggca ccaacatgac
     2341 cttcgtcggc atcaagaagc cggaagatcg cgtcgccctg atcgcctatc tgcacagcct
     2401 gggctcgacc ctgccggtgc cagctcccaa gcccgccgaa gcggccgccc cggctgccgc
     2461 cgaaggcgct ccggccacgg ctgaggaagc tggcgcagcc gccgctggcg cccctgtctc
     2521 gccgtccgag gcccc
//
LOCUS       DAOMEO010000417         2532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4408_length_2532_cov_2.293096, whole genome shotgun sequence.
ACCESSION   DAOMEO010000417 DAOMEO010000000
VERSION     DAOMEO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4408_length_2532_cov_2.293096"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLH31_09945"
     CDS             <1..253
                     /locus_tag="PLH31_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR89563.1"
                     /translation="DAPPKPVYISIQQSGKVYIGDFETTVDEMGQDLAKNIGRRDPTK
                     ERIYIRADEKTRYGAFMEVMNTLQDNGFYSVALIGREDN"
     gene            264..>399
                     /locus_tag="PLH31_09950"
     CDS             264..>399
                     /locus_tag="PLH31_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR89564.1"
                     /translation="MAEQQTPEHRHYDPLTSDGPRRKKGLGAFGVATLVVVGVHAALF
                     A"
     assembly_gap    400..409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <410..>759
                     /locus_tag="PLH31_09955"
     CDS             <410..>759
                     /locus_tag="PLH31_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR89565.1"
                     /translation="FESVMKQYSDDAVDVELPPPPPPPPPPPPPPPPTNEPPPPPAVV
                     QPRPPIAPPPDITPPPPLPIPPVEKRVEQTAPPVISAAPPVAANPPARASVITRPDWA
                     RKPSGDDMARYYPD"
     assembly_gap    760..769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <770..1004
                     /locus_tag="PLH31_09960"
     CDS             <770..1004
                     /locus_tag="PLH31_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR89566.1"
                     /translation="DRAQRMEVNGRATISCTVTAKGALVGCSVVSENPADYGFGEAAL
                     RLSRLFRMKPKTLDGSPVDGGAITVPIVFQVPQ"
     gene            1132..1208
                     /locus_tag="PLH31_09965"
     tRNA            1132..1208
                     /locus_tag="PLH31_09965"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1166..1168,aa:His,seq:gtg)
     gene            complement(1283..>1735)
                     /locus_tag="PLH31_09970"
     CDS             complement(1283..>1735)
                     /locus_tag="PLH31_09970"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515641.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQR89567.1"
                     /translation="AGNSSGVVDGAAAVLLASPAYAAAHGLKPRARVVAMANMGDSPT
                     LMLNAPVPAARKVLAKAGLTVEDIDLWEINEAFAVVAEKFIRDLNLDREKVNVNGGAM
                     ALGHPIGATGSILIGTLLDELERRDLKRGLVTMCAAGGMAPAIIIERV"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1746..2531)
                     /locus_tag="PLH31_09975"
     CDS             complement(<1746..2531)
                     /locus_tag="PLH31_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQR89568.1"
                     /translation="MPEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLRALAARN
                     NLNTAEVDDIVWGTSTQRGKQGGDLGRMAALDAGYDVRASGMTLDRFCGSGITTVNIA
                     AASIMSGMEDLVIAGGTEMMSYTAATADRSAVGLLDSGNLSLRARHPQSHQGVCADAI
                     ATLEGIPRQALDELAVVSQQRADKAIREGRFDRALVPVLRDDGSVALDREEFPRPQTT
                     LESLSQLKASFEMMAGVPIDESGMTLGGLIHQVYPDLKITHVHH"
BASE COUNT          426 a          816 c          815 g          445 t
ORIGIN      
        1 ggatgccccg ccgaagccgg tctacatctc catccagcag tcgggcaaag tctacattgg
       61 cgactttgag acgacagtgg atgagatggg gcaggatctc gcgaagaaca tcggcagacg
      121 cgatccgacc aaggagcgta tctacatccg ggctgacgag aagacccgct acggagcttt
      181 catggaggtt atgaacaccc tccaggataa cggcttctac agcgtggcgc tcatcggccg
      241 ggaagataac taggggtgaa tgcatggctg aacaacagac acccgagcat cgccactacg
      301 atccgctcac ctcggatggc cctcgacgta aaaagggtct aggcgccttc ggtgtcgcga
      361 ccttggtcgt ggttggtgtt cacgctgctt tgtttgcctn nnnnnnnnng ttcgaatcgg
      421 tgatgaagca gtattcggat gacgccgtag acgtggagct tccgcccccg cctccgccgc
      481 cgccgccgcc gccgccgccg cctccgccga ccaatgagcc tccgcctccg ccggcggtgg
      541 ttcagcccag gcctccgatc gcgccgccgc cggacatcac gccgccgccg ccgctgccga
      601 tccctcccgt tgaaaagcgg gtggaacaga cggctccgcc ggtgatctcg gctgctccgc
      661 cggtggctgc aaatccgccg gcccgcgcct cggtcatcac gcgtcctgac tgggcgcgca
      721 agccgagcgg ggacgacatg gcccggtact acccggatcn nnnnnnnnng gatcgtgcgc
      781 aacgcatgga agttaacggc cgcgcgacaa tctcgtgcac cgtgacggcc aagggggctc
      841 tggtaggctg ctcggtggtc tccgagaacc ctgccgacta tggcttcggt gaagcggcgc
      901 tgcgtctgtc gcgtctcttc cggatgaagc caaagaccct cgacggctcg cccgttgatg
      961 gcggcgccat cacggttccg atcgtcttcc aggtcccgca atagggcctg ggcagaccgg
     1021 aaaagacagg aacgccccgt agctccggct tcggggcgtt ttccgttgtg gtggctgttt
     1081 cgacgcttga tcccgttcgc gcctccgact aaagacactc cctcgcgatg cgctgcctta
     1141 gctcagttgg ttagagcgcc ggtttgtgga accggaggtc ctcagttcaa gcctgagagg
     1201 cagtaccatc gccagagact gaaaaaggcc ccggtgtttg ccggggcctt ttcgtttgtc
     1261 ggttaccgcg ctcgagatgg tgtcagacgc gctcgatgat gatcgcagga gccatgccgc
     1321 ccgccgcgca catggtgacc aggccgcgct tgagatcgcg acgctccagc tcatccagga
     1381 gggtgccgat caggatcgag cccgtcgcgc cgatgggatg acccagcgcc atggcgccgc
     1441 cgttcacatt gaccttctcc cggtcaaggt tcagatcgcg aatgaacttc tcggcgacga
     1501 cggcgaaggc ctcgttgatc tcccacagat cgatgtcctc gacggtcagg ccggccttgg
     1561 ccagcacctt gcgcgcggcc ggcacgggcg cgttgagcat cagggtcggg ctgtcgccca
     1621 tattggccat ggccaccacg cgggcgcggg gtttcagacc gtgcgccgcc gcataggccg
     1681 gagaggccag cagcaccgct gcagcgccat cgaccacgcc cgatgaattg ccggcnnnnn
     1741 nnnnngtggt ggacgtgggt gatcttcagg tccggataga cctgatggat cagtccgccc
     1801 agagtcatgc cggactcgtc gatgggcacg ccggccatca tttcgaacga ggctttcagc
     1861 tgcgacaggc tttccagcgt ggtctggggg cgggggaatt cctcacggtc cagagcgacc
     1921 gatccatcat cgcgcaacac cggaacgagg gctcggtcaa agcgtccctc gcggatcgcc
     1981 ttgtcagcgc gctgctggct gaccacggcc agctcgtcca gcgcctggcg tgggatgcct
     2041 tccagcgtag cgatggcgtc ggcgcagacc ccctggtggc tttgcggatg gcgcgcccgc
     2101 aggctcagat tgccagagtc cagcaggcct accgcagagc ggtcggccgt ggcggcggta
     2161 taggacatca tctcggtccc gccggcgatg accagatcct ccatgcccga catgatcgag
     2221 gcagcggcga tattcaccgt ggtgatgccc gagccgcaga agcggtccag ggtcatgccg
     2281 ctggcgcgca cgtcatagcc ggcgtcgagc gccgccatgc gcccaagatc gccgccctgc
     2341 ttgccgcgct gggtgctggt gccccacacg atatcgtcga cctcggcagt gttcagattg
     2401 ttgcgagcgg cgagcgctcg caggaccgtg gccgccagat gctggggatg gaagtcggcc
     2461 agagcgccct tgccgacctt gccgataccg cgcggggtgc gacaggcgtc gatgatgaga
     2521 gcttcgggca tt
//
LOCUS       DAOMEO010000418         2518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4426_length_2518_cov_2.279740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000418 DAOMEO010000000
VERSION     DAOMEO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_2518_cov_2.279740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..1174
                     /locus_tag="PLH31_09980"
                     /pseudo
     CDS             486..1174
                     /locus_tag="PLH31_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516155.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     assembly_gap    1175..1184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1185..>1611
                     /locus_tag="PLH31_09985"
     CDS             <1185..>1611
                     /locus_tag="PLH31_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HQR89569.1"
                     /translation="VFTPIAWFLTDRVVEPRLGPWGGEADDSLKAELAKSAVTPDEKR
                     GLKLAGLAALGVVALFAALTLIPGFTPLIDETKTGPAQLTPFYSALIAGFMLLFLASG
                     VAFGVGVGTVRSDKDVVIMMADGVRSVAPYIVFAFFAAHF"
     assembly_gap    1612..1621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1622..2085
                     /locus_tag="PLH31_09990"
     CDS             <1622..2085
                     /locus_tag="PLH31_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HQR89570.1"
                     /translation="SRLGPIAAIHGAEALKAMNLPAPLLLVSVLGFSSVLDLFIGSAS
                     AKWSALAPVVVPMFMLLGISPEMTTAAYRMGDSFTNLMTPLMSYFPLVLAMTRRWDSS
                     MGVGSLLALMLPYALAFMVAGVAMTLAWVAFDWPLGPAAQVHYTPPGGLLK"
BASE COUNT          361 a          811 c          871 g          455 t
ORIGIN      
        1 gggccggtac caggcgccct ccccctgcga cggggacagg ctcaagaata cgggcggttt
       61 gagctccgag gattggcgcg atcaactatt ttacaagcga ttgatttcgt tgaactcggc
      121 ttcggaaaat gcggggattg agcgccctct acccccatac tcccgtcatc ccgtggcttg
      181 tccacgggac ccatttgtcg gcttcacggt cggatgatcg ccggtcgctc actgcgcaac
      241 ggaaccatgg gtcccgtgga caagccacgg gatgacggtg atctactgga gaggccccgc
      301 ccgggttcgt caggcttgtc cgggatgaca aatctttgcc gcccgatcca tacgagatag
      361 ccccccagag aggaggacct acgccgcgcg ccccgctcag cggcaagcct ctttaccctg
      421 cgcgccaggc ccgctagcgg tggacgctag ggatgggggt ctctttcaag tcaggcgaac
      481 gctgcatgag tgcttccgaa ccgacgtcgc cggcccggca aaagggcttc ctgggggcgg
      541 ttgaacgatt gggcaatctg ctgcccgagc cggtgatgat cttcgtctgg ctgatcctgg
      601 ggctgatggt cctgtcggcc gtgggccagg ttctgggctg gtcggcctcg atcacctata
      661 gcggcgacga ggccccgccg ttcagcgagc tggaaaacgg cgtcctgacc tatgcggcca
      721 gcagcctgtt ttccgaggcc aatctggccc ggctgttcac cgaaatgccc aagaccctga
      781 cgggctttgc gccgctgggc ctggtgctgg tggtgatcct gggggcggcg gtggccgagc
      841 gctcgggcct gttcagcgcc ctgatccggg ccagcctgcg cgaggcgccc agggcgatcc
      901 tcaccccgct ggtggtggtg atcggcatgg tctcgcacca cgcctcggac gcggcctatg
      961 tggtgttcat cccgctggcg ggcctgctgt acgccgccgt cggccgccat ccgctggccg
     1021 gcatcgccgc aggcttcgcg gccgtgtccg gcgggttcgc cggcaacctg accccgggcc
     1081 agttcgacgt ggtgctgttt ggcttcaccc aggaagcggc gcggatcatc gacccgacct
     1141 ggaccatgaa cccgctgggc aactggtggt acatnnnnnn nnnnggtgtt cacgcccatc
     1201 gcctggttcc tgaccgaccg ggtggtcgag ccgcgcctgg gcccctgggg cggcgaggcc
     1261 gatgacagcc tgaaggccga actggccaag tcggcggtga cgccggacga aaagcgcggc
     1321 ctgaagctgg ccggtctggc ggctctgggc gtggtggcgc tgtttgcggc cctgaccctg
     1381 atcccgggct tcacccccct gatcgacgag accaagaccg gcccggccca gctgacgccg
     1441 ttctacagcg ccctgatcgc cggcttcatg ctgctgttcc tggccagcgg cgtggccttt
     1501 ggcgtgggag tcggcacggt gcggtcggac aaggacgtgg tgatcatgat ggccgacggg
     1561 gtgcgctcgg tggcgcccta tatcgtgttc gccttcttcg ccgcccattt cnnnnnnnnn
     1621 nggtcgcggc tgggccccat cgccgccatc cacggggccg aggccctgaa ggcgatgaac
     1681 ctgccggccc cgctgctgct ggtcagcgtg ctgggcttct cgtcggtgct agacctgttc
     1741 atcggctcgg cctcggccaa gtggagcgcc ctggccccgg tggtggtgcc gatgttcatg
     1801 ctgctgggca tctcgccgga aatgaccacc gccgcctatc ggatgggcga cagcttcacc
     1861 aacctgatga ccccgctgat gagctatttc ccgctggtcc tggccatgac ccggcggtgg
     1921 gacagcagca tgggggtggg ctcgctgctg gccctgatgc tgccctacgc cctggccttc
     1981 atggtcgccg gcgtggccat gaccctggcc tgggtggcct tcgactggcc gctggggccg
     2041 gcggcgcagg tgcattacac gccgccgggg gggttgttga agtagggggt gacggggcaa
     2101 tcccacatac ccctccgtca ttccgagcct tcggcccggg agctctcgat ccgccgcagg
     2161 tgcggaaggg gcgaagtgcg cgcgcacttc gctggcgctt cacgtgcgag ctgaaacaga
     2221 ggttcccgcc acgagggcgg gaatgacggt tggaaggggt gtgtgaaacg gaaagccgcc
     2281 agcggcgagg ccccgcccta aatccttgga ggcgagcttg ctctgaggac aggccgtgtc
     2341 aatgaccggc cagaggccgg ccgcgcgcgg cggcgcggcg cgccgtcatt gacacggcct
     2401 gacctcagag ctccaattcc accaagggtt tcagggcgcg ccagcctccg ctgcggcggg
     2461 tggggggggg tagctatcgt cgtgttctac ttaaaaaaag cgtgcgggag acgcggta
//
LOCUS       DAOMEO010000419         2516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4427_length_2516_cov_2.696059, whole genome shotgun sequence.
ACCESSION   DAOMEO010000419 DAOMEO010000000
VERSION     DAOMEO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4427_length_2516_cov_2.696059"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>989)
                     /locus_tag="PLH31_09995"
     CDS             complement(131..>989)
                     /locus_tag="PLH31_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HQR89571.1"
                     /translation="TFAWPTHPEEAEAILDHFFDDMLAGFGDWQDAMSWGRPFLWHSL
                     ISPALNIGLLDPLDICRRAEAAYRAGKAPLNAVEGFIRQILGWREFVRGVYWLKMPEY
                     AKRNALDAEGKLPWFYWTGETDMACVADAVTAARDHAYAHHIQRLMVTGNLAMLLGVH
                     PDAVDDWYMVVFADAYEWVEMPNTRGMATFADGGIVGSKPYAASGAYIDRMSDYCKGC
                     RYDVKDRLGDNACPFNALYWDFMDRHETRLAGQGRLMMPLKTLSRMPDAERAAFRAKA
                     AATRKAMGV"
     assembly_gap    990..999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1000..1666)
                     /locus_tag="PLH31_10000"
     CDS             complement(<1000..1666)
                     /locus_tag="PLH31_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HQR89572.1"
                     /translation="MTDQAQGALRLVLGDQLSDNLSALEGLDPDCDLVLMTESVAEAT
                     AWKHHKQKLVLVWSAMRQFAERLRARGVRVRYVTLDDPANTRSIGGEISRALAEEPLG
                     RVIRTACGKWGLEDHLLTLAKTLPVPMETREDDRFLCSRRQFAAWAEGRKELRMEYFY
                     REMRRSTGLLMDGDKPVGGQWNYDAENRKKLPAGVRPPARLRTPPNATTREVMAMVGS
                     GFDD"
     gene            1785..>2516
                     /locus_tag="PLH31_10005"
     CDS             1785..>2516
                     /locus_tag="PLH31_10005"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQR89573.1"
                     /translation="MTIQSSVLDAIGDTPLIRLNRASEATGCEILGKAEFMNPGQSVK
                     DRAALGIIRDAEARGLLKPGGRIVEGTAGNTGIGLAMVASALGYKTTIVIPRTQSQEK
                     KDAIRLLGAQLVEVDAVPYSNPDNYVRYSGRLAEELAKTEPNGVIWANQFDNLANREA
                     HYRTTGPEIFEQTDGKVDGFICAVGSGGTLAGVAAALRERKPSVKIGLADPHGAALYS
                     WYKNGALKSEGTSISEGIGQGRVTAN"
BASE COUNT          432 a          874 c          802 g          398 t
ORIGIN      
        1 gtatcatgat cgacgccgtc gagaaccgcc tggtcgaggt ggagggcctg cacaatgtct
       61 ggtgcgccgc caccaccgcc ggtggcccgc tggcgctgct gaacatcatc gagacccata
      121 tcgccgcagg ctagacgccc atcgccttgc gcgtcgccgc cgccttggcg cggaaggccg
      181 cccgttcggc atcgggcatc cgggacaggg ttttcaacgg catcatcaga cgcccctgcc
      241 ccgccagccg cgtctcgtgg cgatccatga agtcccagta cagggcgtta aaggggcagg
      301 cgttgtcgcc gagccgatcc ttgacgtcgt agcgacagcc tttgcagtag tcgctcatcc
      361 ggtcgatata ggcgccgctg gccgcatagg gcttagagcc cacgatgccg ccatcggcaa
      421 aggtggccat gccgcgcgta ttgggcatct cgacccattc ataggcgtcg gcgaacacca
      481 ccatgtacca gtcgtcgacc gcgtcgggat gcacgcccag cagcatggcc agattgccgg
      541 tgaccatcag ccgctggatg tggtgggcat aggcgtgatc gcgggcggcg gtgacggcgt
      601 cggcgacgca ggccatgtcg gtctcgccgg tccagtagaa ccacggcagc ttgccctcgg
      661 cgtccagggc gttgcgcttg gcgtattccg gcatcttcag ccagtagacc ccgcgcacga
      721 actcgcgcca ccccaggatc tggcggatga agccctcgac ggcgttgagc ggcgccttgc
      781 ctgcccgata ggccgcctcg gcgcggcggc agatgtccag cgggtccagc aggccgatgt
      841 tcagggccgg cgagatcagg ctgtgccaga ggaacggccg cccccagctc atggcgtcct
      901 gccagtcgcc aaagccggcc agcatgtcgt cgaagaagtg atcaaggatc gcttcggctt
      961 cctcgggatg ggttggccag gcaaaggtgn nnnnnnnnng gtcgtcaaag cctgacccga
     1021 ccatcgccat cacttcacgc gtggtggcgt tgggcggcgt gcgtagacgg gccggcggcc
     1081 gcacgccggc gggcagcttc ttgcggttct cggcgtcgta gttccattgc ccgccgaccg
     1141 gcttgtcgcc gtccatcagt aggcccgtgg agcggcgcat ctcgcggtag aaatattcca
     1201 tccgcagttc ctttcgcccc tcggcccagg cggcgaactg ccggcgcgag cacagaaagc
     1261 ggtcgtcctc gcgggtctcc atgggaaccg gcagggtctt ggccagggtc agcaggtgat
     1321 cttccagccc ccacttcccg caggccgtac gaatcacgcg gcccagcggc tcctcggcca
     1381 gggcgcggga aatctcgccg ccgatcgagc gggtgttggc gggatcgtcc agcgtcacat
     1441 agcgaacccg cacgccccgt gccctcagac gctcggcaaa ctggcgcatg gccgaccaga
     1501 ccaggaccag cttctgcttg tgatgcttcc aggctgtggc ctcggccacg gactcggtca
     1561 tcagaaccag gtcgcagtcg ggatccagcc cctccagagc cgagagattg tcgctcaact
     1621 ggtcgcccag caccagccgc agcgcgccct gtgcctgatc cgtcatggtt tgctcacggc
     1681 ctggtccgct ctttaaggaa aactgggctt gcgtcgggtc gccgcgacac gatctaccat
     1741 gctcgcttcg caccgcagcc cttcagcttc accgatccgc gcacatgacc atacaaagca
     1801 gcgtcctgga cgccatcggc gacaccccgc tcatccgcct gaaccgcgcc agcgaggcga
     1861 cgggctgtga gatcctgggc aaggccgagt tcatgaaccc cggccagtcg gtgaaggatc
     1921 gcgccgccct gggcatcatc cgcgacgccg aagcgcgcgg cctgctgaaa ccgggcggcc
     1981 ggatcgtgga aggcacggca ggcaacaccg gcatcggcct ggccatggtc gcctcggcgc
     2041 tgggctacaa gaccacgatc gtcattcccc gcacccagag ccaggaaaag aaggacgcca
     2101 tccgcctgct cggcgcgcaa ctcgtcgagg tggacgccgt cccctattcc aaccccgaca
     2161 actatgtccg ctattccggc cgcctggccg aagagctggc gaagaccgag cccaacggcg
     2221 tcatctgggc aaaccagttc gacaatctcg ccaaccgcga agcccactac cgcaccaccg
     2281 gtcccgaaat cttcgagcag accgacggca aggtcgatgg cttcatctgc gccgtcggct
     2341 cgggcggcac cctggccggc gtcgctgcgg cgctgcgcga gcgcaagccc tcggtgaaga
     2401 tcggcctggc cgacccccac ggcgcggccc tctacagttg gtacaagaac ggcgcgctga
     2461 agtcggaggg aacctcgatc agcgagggca tcggccaggg ccgcgtcacc gccaat
//
LOCUS       DAOMEO010000420         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4436_length_2513_cov_2.474369, whole genome shotgun sequence.
ACCESSION   DAOMEO010000420 DAOMEO010000000
VERSION     DAOMEO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_2513_cov_2.474369"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>1705
                     /locus_tag="PLH31_10010"
     CDS             47..>1705
                     /locus_tag="PLH31_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89574.1"
                     /translation="MTRTILLATTALVLTAGSAFAAEGPQTTAPVGAVAGDASQRGVL
                     VFQPDFFAAQRPNTALDMVGRVPGFAVNDGSGARGFEGAVGNILINSNRPASKNDTGS
                     NVLSRTPASQVERIELIRGGAPGIDMQGYPVIVNVILKTALSRQSVVTWNASLFDGGQ
                     DVFGGSYQFTASEGDRSWGVTLSDGIGTSDSNGPGRTIRRDGAGGIIRDEFYENDSYG
                     GGRSIRGNYSGPVAGGKLDATARYGVGDWQEWSQQSASGVRRESDYLYEDKDGELGLT
                     YTRPIMPRWTLEARAIHEFEISEEVSTGREALNGVVRPEQRFSAEGESSESIARALVR
                     HERSAALTFEAGGEVAYNMLEVRQAFTQGGVAVPLPSASVKVEETRGEAFSKATWRVN
                     PKLTLEGGLRLETSTIKQSGDADQEKSFFYAKPRLLATWTPIANNQLRVRFERELGQL
                     EFGDFAASAELEDETVYGGNVDLEPEQRWISEITYERRFWGEGIVSIGYRHDKIIGVI
                     DRLPLPGGLSATGNIGDGTLDKLSVNIVVPLDRIGIKGGRFTAKN"
     assembly_gap    1706..1715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1716..2048
                     /locus_tag="PLH31_10015"
     CDS             <1716..2048
                     /locus_tag="PLH31_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89575.1"
                     /translation="GIRPTQANIGFQQDLVKAKTQWGVNWLPLLGQGTYDVDQTNVWR
                     GSNYYEAFVEYKPTPTLALRAQLNLWDDFTIRRTVFADRTTRAVAFVEDRAIDPRTFV
                     SLRIRKTF"
     gene            2289..>2513
                     /locus_tag="PLH31_10020"
     CDS             2289..>2513
                     /locus_tag="PLH31_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR89576.1"
                     /translation="MIAPLQQGAADPRLILREEELDSGLELILLAEAALWAAVDAALE
                     AETLGLGRSHWRAAFLLRRRPGIGVQDLSKL"
BASE COUNT          430 a          859 c          835 g          379 t
ORIGIN      
        1 gaaccagacc ggcagacctc tcgacagaaa gtttgagggg gaaccgatga ccagaaccat
       61 cctgctggcc accaccgcgc tagtcctgac cgccggctcg gccttcgccg ccgaggggcc
      121 gcagacgacg gcgccggtcg gtgcggtcgc gggcgacgcc agccagcgcg gcgttctggt
      181 ctttcagccc gacttcttcg ccgcccagcg cccgaatacg gcgctggaca tggtgggccg
      241 cgtccccggg ttcgcggtca atgacggctc gggcgcgcga ggcttcgagg gcgcggtcgg
      301 caacatcctg atcaacagca accggccggc ctcgaaaaac gacaccggct ccaacgtgct
      361 gtcgcgcacc ccggccagcc aggtcgaacg catcgagctg atccgcggcg gcgctccggg
      421 catcgacatg cagggctacc cggtgatcgt gaacgtcatc ctgaagaccg ccctcagccg
      481 ccagtcggtc gtgacctgga acgcctcgct cttcgacggc ggccaggacg tctttggcgg
      541 ctcctaccag ttcaccgcca gcgaaggcga tcgcagctgg ggcgtcaccc tgtcggacgg
      601 catcggcacc agcgactcca acggccccgg ccggacaatt cgccgcgacg gggcgggcgg
      661 gatcatccgc gacgagttct atgagaatga cagctatggc ggcggccgct cgatccgcgg
      721 caactacagt ggtccggtgg caggcggaaa gctggacgcc accgcccgct acggcgtcgg
      781 ggactggcag gaatggagtc agcagagcgc ttcaggggtc cggcgggaaa gtgactacct
      841 ctacgaagac aaggacggcg agctgggcct gacctacacc cggccgatca tgccgcgctg
      901 gactctggag gcccgcgcca tccacgagtt cgagatctct gaggaggtgt cgaccggccg
      961 cgaagccctg aacggcgtcg tccggcccga gcagcgcttc agcgccgagg gcgaatcctc
     1021 ggaatccatc gcccgcgccc tggtccgcca cgaacgctcg gccgccctga ccttcgaggc
     1081 tggcggcgag gtcgcctaca acatgctgga ggtccggcag gcgttcaccc aaggcggcgt
     1141 ggccgtgccc ctaccctcgg cctcggtgaa ggtggaggaa acgcgcggcg aggccttctc
     1201 caaggcgacc tggcgggtga accccaagct gaccctcgag ggcggcctgc ggcttgagac
     1261 ctcgaccatc aagcagtcgg gcgacgccga tcaggagaag agcttcttct acgccaagcc
     1321 ccggctgctg gcgacctgga cgccgatcgc caacaaccag ttgcgggtgc gcttcgaacg
     1381 cgaactgggc cagctggagt tcggtgactt cgccgcctcg gccgagctgg aggacgagac
     1441 cgtctacggc ggcaatgtcg atctggagcc ggaacagcgc tggatctcgg aaatcaccta
     1501 tgagcgccgc ttctggggcg agggcatcgt ctcgatcggc tatcgccatg acaagatcat
     1561 cggcgtcatc gaccgactgc ccctgccggg cggcctgtcg gccacgggca atatcggcga
     1621 cggcacgctg gacaagctgt cggtcaatat cgtcgtgccg ctggacagga tcggcatcaa
     1681 gggcgggcgt ttcacggcca agaacnnnnn nnnnnggcat ccgtcccacc caggccaata
     1741 tcggcttcca gcaggatctg gtgaaggcca agacccagtg gggcgtcaac tggctgccgc
     1801 tgctgggcca gggcacctat gacgtggatc agaccaatgt ctggcgcggc tcgaactatt
     1861 acgaggcctt cgtcgagtac aagccaaccc cgaccctggc cctgcgggcc cagctgaacc
     1921 tgtgggacga cttcaccatc cgccgcacgg tcttcgctga ccgcacgacc cgcgccgtgg
     1981 ccttcgtcga ggatcgggcc atcgatccgc gcaccttcgt cagcctgcgg atccgcaaga
     2041 ccttctgagg ccgccgcatc gcagccgtcc gggagcgatt ctgggcggga gcgatcccgg
     2101 gcggctttcc gttcagggcc acgcgccgaa acagcggtca ttcggctgct gctttcaacg
     2161 atcagacacg cagaacagcc gcgaaggttg ttcttggccg cgctcgcctt gcggggctcg
     2221 ccgatctcgg ccaccgtgtg cgatagacct cctgacagcc tgaccgacaa tcaagtggac
     2281 ccacggcgat gatagccccc ctccagcaag gcgcggccga tccccggctc atcctgcgcg
     2341 aggaggagct ggacagcggc ctggagctga tcctgctggc cgaggctgcc ctgtgggcgg
     2401 cggttgatgc tgcgctggag gccgagacgc ttggcctggg ccgctcgcac tggcgcgccg
     2461 ccttcctgct gcgccgccgg cccggcatcg gggtgcagga cctgtcgaaa ctg
//
LOCUS       DAOMEO010000421         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4440_length_2511_cov_2.211726, whole genome shotgun sequence.
ACCESSION   DAOMEO010000421 DAOMEO010000000
VERSION     DAOMEO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_2511_cov_2.211726"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLH31_10025"
     CDS             <1..641
                     /locus_tag="PLH31_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR89577.1"
                     /translation="KTINIAVVTTGYPNIIQPTLVMSRDTLRMLGEADTGQRVGPLMV
                     QIRDPARAEIVAAQLNKKADGKFRAWTRQELADANSSAMLDDQIIGIMLGFSAFLGLL
                     IGVAITWQTLRGAIMANIKEFASLRALGVSMGDLRNIVMELSFWVGIVGVGAAGLLTW
                     LVTLLATAGGVPMFFPPSLIIIVAVFLVVIAMLSGFLSLGILKNSQPADLLR"
     gene            692..1387
                     /locus_tag="PLH31_10030"
     CDS             692..1387
                     /locus_tag="PLH31_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR89578.1"
                     /translation="MVKRFKTGRTFIEVLKGVDFDAKHGDVTMVMGPSGSGKSTLVAA
                     LSGLLKPDEGKVSALDAKDLWSLSSGKIDRFRLDHCGFIFQGFNLFPALTAKQQVMTV
                     LKYQSIGPGEAARRAQAALESVGLGPRVNQRPSELSGGEKQRVAIARALAKNPNLIFA
                     DEPTSALDGKSGETVIKLLRAAATERGAAVICVTHDPRLEAYADRVIHIEDGRILDDV
                     RRTPDANPASLSH"
     gene            1405..>2030
                     /locus_tag="PLH31_10035"
     CDS             1405..>2030
                     /locus_tag="PLH31_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR89579.1"
                     /translation="MFGFLRRPIFWIVIVVLAAGGGGFFYMQGQAAEKKKKLEVAAAQ
                     EEPSPYAAIANGKADVEGGVIQVAARRQGIVREVFVQEGDVVVAGQPLAKQEDDEPRL
                     AAQTASANLASAKAQLALYQVQLRTAQREYKRLQGLAASNFVAAQKLDAARDQIAQAE
                     ANIGAQRAAIGVSSAQLGQAQYQQELTVIRAPANGRIARRYANPGAGAS"
     assembly_gap    2031..2040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2041..2424
                     /locus_tag="PLH31_10040"
     CDS             <2041..2424
                     /locus_tag="PLH31_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin secretion protein D"
                     /protein_id="HQR89580.1"
                     /translation="ASTLNVTSMFDLEPDTQRIVRAEIVEADIPNVTNNQEVEITPES
                     DPDKVFLGKVLRRAAVFGARKLASDDPSQRSDERVVEVVVSADGAPLLVGQRVLVKFM
                     KPGQKAGVKRAKPAAPVGGGMTKPK"
BASE COUNT          428 a          871 c          815 g          387 t
ORIGIN      
        1 gcaagaccat caatatcgcc gtggtcacca ccggctatcc caacatcatc cagccgaccc
       61 tggtgatgtc gcgcgacacc ctgcggatgc tgggcgaggc cgacaccggc cagcgcgtcg
      121 gtccgctgat ggtgcagatc cgcgatccgg cccgcgccga gatcgtggcg gcccagctga
      181 acaagaaggc cgacggcaag ttccgcgcct ggacccgtca ggaactggcc gacgccaatt
      241 ccagcgccat gctggacgac cagatcatcg gcatcatgct gggcttctcg gccttcctgg
      301 gcctgctgat cggcgtggcc atcacctggc agaccctgcg cggcgcgatc atggccaata
      361 tcaaggagtt tgccagcctt cgcgccctgg gcgtgtcgat gggcgacctg cgcaacatcg
      421 tcatggagct gagcttctgg gtcggcatcg tcggcgtcgg cgcggcgggc ctgctgacct
      481 ggctggtcac cctgctggcc accgccggcg gcgtgccgat gttcttcccg cccagcctga
      541 tcatcatcgt cgcggtcttc ctcgtggtga tcgccatgct gtctggcttc ctgtcgctgg
      601 gcatcctgaa gaacagccaa cctgcggacc tgctgcgatg agcaatgatc acagccacaa
      661 gcgcggctag agcgctctct cggcccaggg catcgtcaag cggttcaaga ccggccgcac
      721 cttcatcgag gtgctcaagg gcgtggactt cgacgccaag cacggcgatg tgaccatggt
      781 catgggcccc tcgggctcgg gcaagtccac cctggtggcg gccctgtcgg ggctgctgaa
      841 gcccgacgag ggcaaggtgt cggcgctgga cgccaaggac ctgtggtcac tgtcatcggg
      901 caagatcgac aggttccgcc tcgatcactg cggctttatc ttccagggct tcaacctgtt
      961 cccggctctc actgccaagc agcaggtgat gaccgtcctg aagtatcaga gcatcgggcc
     1021 cggcgaagcc gcgcggcgcg cccaggcggc gctggagtcg gtgggccttg gcccgcgcgt
     1081 caaccagcgc ccctcggaac tgtcgggcgg cgagaagcag cgggtggcca tcgcccgggc
     1141 gctggccaag aatcccaacc tgatcttcgc cgacgagccc acctcggcgc tcgacggcaa
     1201 gagcggtgaa accgtcatca agctcctgcg cgccgccgcg acggagcgcg gcgcggcggt
     1261 gatctgcgtc acccacgacc cgcggttgga ggcctatgcc gaccgcgtca tccacatcga
     1321 agacggccgg atcctggacg acgtgcgtag aacgcccgac gccaaccccg cgtcgctcag
     1381 ccactgattt ttggggaaac tgatatgttc ggctttctgc gccgcccgat attctggatc
     1441 gtcatcgtcg tgctcgccgc aggcggcggc gggttcttct acatgcaagg ccaggcggcc
     1501 gagaaaaaga agaagctcga agtggcggcg gctcaggaag agccctcccc ctatgcggcc
     1561 atcgccaacg gcaaggccga tgtcgagggc ggcgtgatcc aggtggccgc gcgccgccag
     1621 ggcatcgtcc gcgaggtgtt cgtccaggaa ggcgacgtgg tggtcgccgg ccagcccctg
     1681 gccaagcagg aagacgacga gccgcgcctt gccgcccaga ccgcttcggc caacctggcc
     1741 tcggccaagg cccaactggc cctctatcag gtgcagctgc gcaccgccca gcgcgaatac
     1801 aagcggctac agggcctggc ggcgtccaac ttcgtggccg cccagaagct cgatgcggcc
     1861 cgggaccaga tcgcccaggc cgaggccaat atcggcgccc agcgcgccgc catcggcgtg
     1921 tcgagcgccc agctgggtca ggcccagtac cagcaggagc taacggtgat ccgcgcgcca
     1981 gccaacggcc gcatcgcgcg tcgctacgcc aatccgggcg ccggcgcctc nnnnnnnnnn
     2041 gcctcgaccc tgaacgtcac ctcgatgttc gacctcgagc ccgacaccca gcgcatcgtt
     2101 cgcgccgaga tcgtcgaggc cgatatcccc aacgtgacca acaaccagga agtcgagatc
     2161 accccggaaa gcgatcctga caaggtattc ctcggcaagg tcctgcgtcg cgccgccgtg
     2221 ttcggggccc gcaagctggc ctccgacgat cccagccagc gcagcgacga gcgcgtggtc
     2281 gaggtggtgg tcagcgccga cggcgccccg ctgctggtgg gccagcgcgt gctggtcaag
     2341 ttcatgaagc ccggccagaa ggccggcgtg aaacgcgcca agccagccgc gccggtgggc
     2401 ggcgggatga ccaagccgaa gtagaacgag gtcatctgct ccccacgggg aagatcacat
     2461 caagcgccgc gcggctcgtc cgcgcggcgt tcttcgtttt ggccaccctt g
//
LOCUS       DAOMEO010000422         2502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4463_length_2502_cov_1.925623, whole genome shotgun sequence.
ACCESSION   DAOMEO010000422 DAOMEO010000000
VERSION     DAOMEO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_2502_cov_1.925623"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLH31_10045"
     CDS             <1..315
                     /locus_tag="PLH31_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89581.1"
                     /translation="SLDLGAGGQMTIQPQTEFFMLGLGYGHPAWAHGLNHGDLAVERE
                     DFVTAELERRLPHHLHVQALSRVVFTNAQGRSRIGRGVFEQLVLGPHAPSGFTSILDV
                     AP"
     gene            312..>978
                     /locus_tag="PLH31_10050"
     CDS             312..>978
                     /locus_tag="PLH31_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQR89582.1"
                     /translation="MTLAERLEAYLTRHFGAPTTVRDLSRIPGGASRETYRFDAVIDG
                     AVRPLILRRDPAASLIETDRSLEYLAFQSFHDRAPVPEPIVLEAEGAELERPFFIMAR
                     VDGGAAASPFTVQPYGAHAQAIGEAFFAALGAIAATDPAGLPLARAATMPAPGDCWRV
                     ALDHWADVIEADEQHPQPIVRAAIRRLRRAPPPPAAAVRVVHGDYRTGNFLHDGQGKI
                     LAIL"
     assembly_gap    979..988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <989..1322
                     /locus_tag="PLH31_10055"
     CDS             <989..1322
                     /locus_tag="PLH31_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQR89583.1"
                     /translation="AHLGEPLEDLAWAIDPLWGHYDETRVGGMIPESEALAIWSHTSG
                     LSIDPDALAWWRLFSAVKGQAIWTSAAREYRDGGFKDPVLAFSGWYTARRHDQILADG
                     LMRLEGLS"
     gene            1319..1750
                     /locus_tag="PLH31_10060"
     CDS             1319..1750
                     /locus_tag="PLH31_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89584.1"
                     /translation="MTPSLPDILVGNFLCLAEAPPPESAGEFTAGKVGATALLSLLAA
                     QEAERGLTARVWENGAIRALVSDAAPDYGQGFAEAASGPDGDFSLASLDRVNALLRRA
                     LISLHEAVEAAGDTARDQAILRLYRDMAHARRLDMPPLPGR"
     gene            complement(1754..2478)
                     /locus_tag="PLH31_10065"
                     /pseudo
     CDS             complement(1754..2478)
                     /locus_tag="PLH31_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516668.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
BASE COUNT          393 a          842 c          851 g          406 t
ORIGIN      
        1 agtcttgatc tcggcgccgg cggccagatg acgatccagc cccagaccga attcttcatg
       61 ctgggcctcg gctatggtca cccggcctgg gcccacggcc tcaaccacgg ggatctcgcc
      121 gtggaacgcg aggactttgt gaccgccgag ctggaacgtc gcctgccgca tcacctgcac
      181 gtccaggcgc tgagccgcgt ggtcttcacg aacgcgcagg gacgcagtcg gatcggaagg
      241 ggggtgttcg agcaactggt gctcgggccg cacgcgccct caggcttcac gtcgatcctg
      301 gatgtcgctc catgaccctg gccgagcggc tcgaagccta cctgacccgc cacttcggtg
      361 cgccgacaac ggtgcgggac ctgtcgcgca ttccaggcgg ggccagccgc gagacctatc
      421 gcttcgacgc cgtgatcgat ggcgcggttc ggccgctgat ccttcgacgc gacccggccg
      481 caagcctgat cgagaccgat cgcagcctcg aatatctggc tttccagtcc ttccacgacc
      541 gcgcgcccgt gcctgagccg atcgtgctgg aagccgaagg cgcggagctg gagcggccgt
      601 tcttcatcat ggctcgggtc gatggcgggg cggcggcttc gccctttacg gtccagccct
      661 atggcgcgca cgctcaggcg atcggcgagg cgttcttcgc ggctctgggc gccatcgcgg
      721 cgaccgatcc cgcgggtctg ccgctggcca gggccgcgac catgcccgcg ccgggcgact
      781 gctggcgcgt ggcgctggac cactgggccg atgtgatcga ggccgacgaa cagcaccccc
      841 agcccatcgt gcgcgccgcc atccgccgtc tgcgccgcgc gccgccgccg ccggccgccg
      901 ccgtccgggt ggtgcatggc gactatcgca ccggcaattt cctgcatgac ggccagggca
      961 agatcctggc gatcctcgnn nnnnnnnngg cgcatctggg cgagcctctg gaggacctgg
     1021 cctgggcgat cgatccgctc tgggggcact atgacgagac gcgcgtgggc ggcatgatcc
     1081 ccgaatccga agcgctggcg atctggagcc acaccagtgg cctgtcgatt gatcccgacg
     1141 ccctggcctg gtggcgcctg ttcagcgcgg tgaagggcca ggcgatctgg acctcggcgg
     1201 cgcgggaata tcgcgacggc ggcttcaagg atccagtcct ggccttttcc ggctggtaca
     1261 ccgctaggcg acacgaccag atcctggccg atggcctcat gcgcctggag ggcctgtcat
     1321 gaccccgagc ctgcccgaca tcctggttgg caatttcctg tgcctggccg aggcgccgcc
     1381 gccggaatcg gcgggagagt tcacggctgg caaggtgggg gctacggccc tgttgtcctt
     1441 gctggccgcc caggaggccg agcgcgggct gaccgcgcgg gtctgggaaa atggcgcgat
     1501 ccgggccctg gtgtcggacg ccgcgcctga ctacggccaa ggtttcgccg aggccgccag
     1561 cggaccggac ggcgactttt cactggccag ccttgatcgc gtcaacgccc tcctgcgccg
     1621 cgccttgatc agcctgcacg aggctgtcga ggcggcgggg gacaccgcgc gcgatcaggc
     1681 gatcctgcgc ctctatcgcg acatggccca tgcccggcgt ctggacatgc cgccactgcc
     1741 gggacggtga agcctacttc ttgtgcttca tgtgatcggc cagttcgtct ggaccgatat
     1801 cgatgaaggc tggcgcggag gcgttgtagg cgctggtctt gtccagatcc atgaccacgc
     1861 tgcgatggcc ttcccagatc atgtgcatgg ccacatacag cacgatggcc aggccgacga
     1921 agccgatcca gcgatgcttg tgcagcaggt tggcgatggc cgaggcggcg acgcccatca
     1981 ggatgatcga cagcagcagg ccaaagacca ggatgcgccg gccacggcca gcacgttgtc
     2041 gagtgacatc gagacgtcgg cgatcagcac ctggatgaag gcttgcttga aggtcttgac
     2101 gggtcgagcg cgcggttcgg tggtcgggtc cccgtcgatc gaggcgacgg cgtcggcctg
     2161 atcatgggtg gcctgctcgc gcagctctcg ccacatcttc cagcagaccc agagcagcag
     2221 tacgccgccg gccagcagca ggccgatgat gcccagcaac caggtggtga tcagcgcaaa
     2281 gccgatgcgc agcaccacgg ctgcgccaag tccgtacaga atgaccttct ggcgttgctt
     2341 gaccggcaaa ccgcccgccg cgaggccgac ggccacggcg ttgtcgccgg ccagaaccag
     2401 gtcgatcatc aggacttgca gaaaggcgaa gagggggcct tccagcccgc ctgcctgggc
     2461 gatcagagcg tcaaacatga cctctcatgc ggcagggcgg gc
//
LOCUS       DAOMEO010000423         2500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4469_length_2500_cov_2.797546, whole genome shotgun sequence.
ACCESSION   DAOMEO010000423 DAOMEO010000000
VERSION     DAOMEO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_2500_cov_2.797546"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1064
                     /gene="ctrA"
                     /locus_tag="PLH31_10070"
     CDS             369..1064
                     /gene="ctrA"
                     /locus_tag="PLH31_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle two-component system response
                     regulator CtrA"
                     /protein_id="HQR89585.1"
                     /translation="MRVLLIEDDSATAQTIELMLKSEGFNVYTTDLGEEGVDLGKIYD
                     YDLILLDLNLPDMSGLDVLRTLRVAKVNTPIMILSGSSEIDTKVKTFSGGADDYMTKP
                     FHKDEMIARIHAVVRRSKGHAQSVIKTGDIVVNLDAKTVEVNSNRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAASAQGKHHIETVWGRG
                     YVLRDPNEQVNAA"
     gene            1313..>2500
                     /locus_tag="PLH31_10075"
     CDS             1313..>2500
                     /locus_tag="PLH31_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR89586.1"
                     /translation="MQEFDPRRPTLRLAPRLFTAVAGMAALGLGWKLTAAEVPTPVKA
                     ALDPAALIALQHVAFASAEAQPGFDRPQSIPVKVRPGETLEGAVQRAGVAPEEARQVV
                     AALHGAIDTVNIKAGMAFEAAIAQRRSERGPARLIGLSMRTSPSSTLTVSRTFDGALK
                     LRELEEEVREETKVACGQMNGSFYESVANVGGTPRVISDAAKLFAHKIDFSRDIRDGD
                     KFCLVFDRKVTESGRTIEAGDLEYAEVKGQRFYGFERDGKTQFFDEAGKNIKGFLLRT
                     PVDGARITSTFGARRHPVLGYNRAHKGVDFGAGHGTPILAAGDGVVLEARRWAGYGNW
                     LRIRHAGKWDTGYAHISRYAPGIRPGMRVRQGQVVAYVGSTGMSTGPHLHYEVWLNGQ
                     RVNP"
BASE COUNT          500 a          813 c          763 g          424 t
ORIGIN      
        1 cgcggacgtg gcgaagagcc ggcaaaaatc ctagcgacag aacgccgcaa tcgcgccgca
       61 atacgcaggc ctcccgactg gcggcggggc agggcccagg actccagaac cgttaaggcg
      121 ctgggatctg acgattcgcc ggttggggga atccgcttta ggggccttga gaaccatccc
      181 cgaccaattg ccggtgcgac tcagcgaccg cttgcacccg attcgcaaat catattaacc
      241 attcggacat cgagatcatt caccattaac cagtcttaaa ttaactgtcc cgcaaagtgc
      301 aggacgagtt taacaggcgg cttccgccaa gacgtcgaat aacgccacaa agtggcgtgg
      361 aggggctcat gcgcgtactg ttgatcgagg atgacagcgc aactgcgcag accatcgaac
      421 tgatgctgaa gtctgaaggc ttcaacgtct acacgacgga tctcggtgaa gaaggcgtcg
      481 atctgggcaa gatctacgac tacgatctta tcctgctcga cctgaacctg cccgacatga
      541 gcggcctgga tgttctgcgc accctgcggg tggcgaaggt gaacacgccg atcatgatct
      601 tgtcgggttc gtcggaaatc gacacgaagg tcaagacctt ctcgggcggc gccgacgact
      661 acatgaccaa gcccttccac aaggacgaga tgatcgcccg catccatgcg gtggtccgtc
      721 gttcgaaggg gcacgcccag tcggtcatca agaccggcga catcgtggtc aacctcgacg
      781 ccaagacggt cgaagtgaac agcaaccgcg tccacctgac cggtaaggaa tatcagatgc
      841 tggagctgct ctccctgcgc aagggcacga ccctgaccaa ggagatgttc ctcaaccacc
      901 tttacggcgg catggacgag ccggagctga agatcatcga cgtcttcatc tgcaagcttc
      961 gcaagaagct ggcggcctcg gctcagggca agcaccatat cgagacggtt tggggccgcg
     1021 gctacgtgct tcgcgatccg aacgagcagg tcaacgccgc ctgatcggcg cgacgacctt
     1081 ccgaacaaag aaagcgcccg cccccaaagg cgggcgtttt tcctttcggg atcatcagct
     1141 tgaccttatg tcggctgaac ctgttaacct cgtccgtgac aaaacgaccg aagcatcgaa
     1201 tagtacttgc cgccggaagg gcgcacggcc atgttccggc gcaatgattg ctggggggct
     1261 acccagccgt gtcattttga ccttgcgcgg catcgaaacc gatgggggac cgatgcaaga
     1321 atttgatccg cgacgcccga ctcttcgtct ggcgccccgg ctgttcacag ccgtcgccgg
     1381 catggccgct ctgggcctgg gctggaaact gacggccgct gaagtcccga ctccggtcaa
     1441 ggccgccctc gaccccgccg cactcatcgc cctgcaacat gtcgccttcg ccagcgctga
     1501 agcccagccc ggcttcgacc gcccgcagag cattcccgtg aaggtccgcc ctggcgagac
     1561 cctggagggc gctgtccagc gcgccggcgt cgcgcccgaa gaggcccgcc aggtggtcgc
     1621 cgccctgcat ggcgccatcg acaccgtcaa catcaaggcc ggcatggcct ttgaagccgc
     1681 gatcgcgcag cgtcgcagcg agcgtggccc tgcccgcctc atcggcctgt cgatgcgaac
     1741 cagcccgtcc tcgaccctga ccgtttctcg cacctttgat ggcgcgctga agctgcgcga
     1801 gctcgaggaa gaggtccgtg aagagaccaa ggtcgcctgc ggccagatga atggctcgtt
     1861 ctatgagagc gtcgccaacg tggggggaac gccccgcgtg atcagcgacg cagccaagct
     1921 gtttgcgcac aagatcgact tctcgcgcga cattcgcgac ggcgacaagt tctgcctggt
     1981 gttcgaccgc aaggtcaccg aaagcggccg caccatcgag gccggcgacc tggaatatgc
     2041 cgaggtcaag ggccagcgct tctacggctt cgagcgcgac ggcaagaccc agttctttga
     2101 cgaagccggc aagaacatca aaggcttcct gctgcgcaca ccggtcgacg gcgcccgcat
     2161 cacctcgacc ttcggggccc gtcgccaccc tgtgctgggc tacaaccgtg cgcacaaggg
     2221 cgtggatttc ggagccgggc acggcacgcc gatcctggcg gctggcgacg gcgtcgtgct
     2281 ggaggcacgt cgctgggctg gctatggcaa ctggctgcgc atccgtcatg cgggcaagtg
     2341 ggacaccggc tatgcccaca tctcgcggta tgcgcccggc atccgccccg gcatgcgcgt
     2401 ccgccagggc caggtcgtgg cctatgtcgg ctcgaccggc atgtcgaccg gcccgcacct
     2461 gcactatgaa gtctggctga acggccagcg ggtcaatccg
//
LOCUS       DAOMEO010000424         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4482_length_2493_cov_2.534865, whole genome shotgun sequence.
ACCESSION   DAOMEO010000424 DAOMEO010000000
VERSION     DAOMEO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4482_length_2493_cov_2.534865"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLH31_10080"
     CDS             complement(<1..492)
                     /locus_tag="PLH31_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR89587.1"
                     /translation="MARPFAYAALAAALLTAPAALAAPGVNSTKPADLPAGNYVLDKT
                     HAVLTAKLSHMGFSNYTLRFTKLDANFTYDPKAPEASKISVTVDPASIDTSTGADAFG
                     LKFNKELAGDGWLETAKYPTITFISTKVDVGDGRNGKVTGDLTFHGVTKPVVLDVTFN
                     GVGS"
     gene            673..2454
                     /locus_tag="PLH31_10085"
     CDS             673..2454
                     /locus_tag="PLH31_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQR89588.1"
                     /translation="MTYRAPVRDIAFSLRHAAGFDRLSGAFPDADAETVQAVLDGAGA
                     LAGDILAPLNRTGDLTGARLENGVVRTAPGFGEAYQQFAQGGWNSLAADPAHGGQGLP
                     KSLEIGVFEMVHAANMALGLCPMLTLGAIEALAHHGSDRQKALYLPRLVSGAWTGTMN
                     LTEPQAGSDLAAIATLATPDATGGWSITGQKIYITWGDHDVADNIVHLVLARTPDAPP
                     GVKGISLFLAPKMLVGEDGSLGAANALRAGGLEHKLGIHGSPTCVMLFEGARAELVGE
                     LGKGLAHMFTMMNAARLQVGTQGVAIAERAYQQALAFSLERRQGRSAWTGENAARLFD
                     HPDVRRTLMLMKARIEAARGVCLSTAVAADLATHGATEDERVAAKLREELLTPIAKAW
                     STDLGVEVASQGVQIHGGMGFVEETGAAQHYRDARIAPIYEGTNGIQAIDLIGRKLGL
                     GEGQAIGDLMDDIRDTIDALNAAGDPAFGTVAKRLETALDAASAATAWLIERRARAMP
                     DALSGATAYLKLLGDVVGGWMLAKGALAASAAIAAGEGDTGWLGGKLALARLYAEAVL
                     AQVPGQVAGVMLGAADLEATTPEMLGA"
BASE COUNT          396 a          819 c          885 g          393 t
ORIGIN      
        1 gctgccgacg ccattgaagg tgacgtccag aaccaccggc ttggtgacgc cgtggaaggt
       61 caggtcgccc gtgaccttgc cattgcggcc gtcgcccacg tcgaccttgg tcgagatgaa
      121 ggtgatggtc ggatatttgg cggtttccag ccagccgtcg ccggccagtt ccttgttgaa
      181 cttcaggccg aaggcatcgg cgcccgtcga ggtgtcaatc gaggccggat cgaccgtcac
      241 cgagatcttc gaggcttccg gcgccttggg atcataggtg aaattggcgt ccagcttggt
      301 gaaacgcagg gtgtagttgg agaaccccat gtggctgagc ttggcggtca gcacggcgtg
      361 ggtcttgtcc agcacgtagt tgccggccgg caggtcggcg ggcttggtcg agttgacacc
      421 cggcgcggcc agggcggcgg gcgccgtgag gagcgcggcg gcgagcgcgg cgtaggcgaa
      481 gggacgggcc atgaggtggg atctccaaac tgtgattgat gcagttacga tttccccgac
      541 ttaagaagtc cagcgcaagg ataaagggct gtaacatcac gaagccgtga cttcttgaac
      601 gaagggcacg cccgcttggc cctggcgacg cacgcgccta aagaaacgcc gattatctgg
      661 agtctagcgt ttatgaccta ccgtgcaccc gtccgcgaca tcgccttttc acttcggcac
      721 gcggccggct ttgaccgcct gtccggcgcc tttcctgacg ccgacgccga gacggtgcag
      781 gcggtgctcg acggggccgg cgccctggcg ggcgacatcc tggcgccgct gaaccgtacg
      841 ggtgatctga ccggcgcccg actggaaaat ggcgtggtcc gtaccgctcc aggctttggc
      901 gaggcctatc agcagttcgc gcagggcggc tggaacagtc tggccgccga tccggctcat
      961 ggcggccagg gcctgcccaa gagtctggag atcggcgtct tcgaaatggt gcatgccgcc
     1021 aacatggcgc ttggcctctg cccgatgctg accctaggcg caatcgaggc cctggcccac
     1081 cacggctccg accgccagaa ggcgctctat ctgccccgcc tggtctcggg cgcgtggacg
     1141 ggcacgatga acctgaccga accccaggct ggctcggacc tggcggcgat cgccaccctg
     1201 gccacgccgg acgcgacggg cggctggtcg atcacgggcc agaagatcta catcacctgg
     1261 ggcgaccacg acgtggccga caacatcgtc catctcgttc tggcccgcac gcccgacgcc
     1321 ccgcccggcg tgaagggcat ctcgctgttc ctggccccca agatgctggt cggcgaggac
     1381 ggttccctgg gcgcggccaa cgccctgcgg gccggcgggc tggagcacaa gctgggcatc
     1441 cacggctcgc ccacctgcgt gatgctgttc gagggcgcca gggcagagct ggtcggcgaa
     1501 ctgggcaagg gcctggccca catgttcacg atgatgaacg ccgcgcgcct gcaggtgggg
     1561 acgcagggcg tggccattgc cgagcgcgcc tatcagcagg ccctggcctt cagcctggaa
     1621 cgccgtcagg gccgctcggc ctggaccggt gaaaacgccg cccgactgtt cgaccatccg
     1681 gacgtccgcc gcacgctgat gctgatgaag gccaggatcg aggcggcgcg cggcgtctgc
     1741 ctgtccaccg ccgtcgccgc tgacctggcc acccacggcg ccaccgagga tgagcgcgtc
     1801 gccgccaagc tgcgcgagga gctgctgacc ccgatcgcca aggcctggtc gaccgacctc
     1861 ggcgtcgagg tcgcctcgca gggcgtgcag atccacggcg gcatgggctt tgtcgaggaa
     1921 accggcgcgg cccagcacta tcgcgacgcc cgcatcgccc cgatctatga ggggaccaac
     1981 ggcatccagg ccatcgacct gatcggccgc aagctgggcc tcggcgaagg tcaggccatc
     2041 ggcgacctga tggacgatat ccgcgacacg atcgacgccc tgaacgccgc aggcgacccg
     2101 gctttcggca cggtcgccaa acgcctggag acggctctgg acgccgccag cgccgcgacc
     2161 gcctggctga tcgaacgccg cgcgcgggcc atgcccgacg ccctgtcggg cgcgacggcc
     2221 tatctgaaac tgctgggcga tgtggtgggc ggctggatgc tggccaaggg cgcgctggcg
     2281 gcctcggcgg ccatcgcggc gggcgaaggc gatacggggt ggctgggcgg caagctggcc
     2341 ctggcgcggc tctatgccga ggcggtgctg gcccaggttc cgggccaggt ggcgggggtc
     2401 atgctcggcg cggcggatct tgaggcgacg acgccggaga tgctgggggc ttaggtcggg
     2461 atagatagat gctccccctc tgggggagct gtc
//
LOCUS       DAOMEO010000425         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4489_length_2489_cov_2.798685, whole genome shotgun sequence.
ACCESSION   DAOMEO010000425 DAOMEO010000000
VERSION     DAOMEO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_2489_cov_2.798685"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..219)
                     /locus_tag="PLH31_10090"
     CDS             complement(25..219)
                     /locus_tag="PLH31_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQR89589.1"
                     /translation="MKWFNTTKGFGFIQPDNGGGDIFVHISAVERAGLRGLNEGQQVG
                     YELEQDRRSGKTSAGNLRVL"
     gene            complement(370..573)
                     /locus_tag="PLH31_10095"
                     /pseudo
     CDS             complement(370..573)
                     /locus_tag="PLH31_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            complement(1049..2368)
                     /locus_tag="PLH31_10100"
     CDS             complement(1049..2368)
                     /locus_tag="PLH31_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5690 family protein"
                     /protein_id="HQR89590.1"
                     /translation="MSSGPRLWLERANPLVFTLFAGLAGFCAYFSMYAFRKPFAAATF
                     GEVEGWTFAIDYKIALVLAQVAGYAASKLIGVKVISEIAPAHRGVAILGLIGMSWLAL
                     LAFAVVPAPWNVVALVLNGLPLGMIWGLVFGFMEGRRTSEVLGAILCASFILSSGVVK
                     SVGKWLMVDLDVSPFWMPAATGALFLPLLAFSVWALVQLPPPSLADEAARVRRQPMDR
                     ARRRAFLAAYAPGVVLLVMAYVLLTALRDFRDNFAAEIWIALGFGEASGVFTTSELPV
                     AAVALAVMGAVMLVRDNLRALLVMHGVILAGFLLLGLSTLAFQADLLTPLTWMILTGA
                     GLYMAYTPFNAMLFDRMIAFSGQVGTAGFLIYVADASGYLGSVALLLWRNFAMVNLDW
                     LGFFIASVYATSIVGAVCTILAGLYFLRRRPTSSASAPTVNAADALA"
BASE COUNT          526 a          799 c          809 g          355 t
ORIGIN      
        1 gcgcgcctgc ccggtccttg atgcttagag gacgcgcagg ttgccggccg aggtcttgcc
       61 cgagcgacgg tcctgttcca gttcgtaacc gacctgctgg ccttcgttca ggccccgcag
      121 accggcgcgc tcaacggcag agatgtgcac gaagatgtcg ccgccgccgt tgtcgggctg
      181 gatgaaacca aagcccttgg tggtgttgaa ccacttcacg acgcccgtgc cggcttcgcc
      241 gccaccgccg ccgccaccac cgccgccgta gccgccgccc cggggagcgt catagccgcc
      301 gccgcgcggg gcgtcgaagc cgcggggagc cgggcgacgg gcagcggcgg gagccgcgcc
      361 ttgaccggtt acgcgcaggt tgccggccga ggtcttgccc gagcgacgat cttcttcgag
      421 ttcataggcg accaggtcgc cttcgttcag gcccgaaagg cccgaccgct caaccgcggc
      481 gatatggacg aacacgtcct cgccgccgtt ttcgggctgg ataaagccaa agcccttggc
      541 cgggttgaac cacttaacga caccgttcgc catcttatcc tcaaaaacct attccgtggg
      601 ccagttgccg acccgcgcct tccatagcat ctcatgacgc aaatcaaagg atcgcgccat
      661 gggctttgga tggagattct cgaagaacga tgtgcgaagt gcagaaaatt tcgcttccgg
      721 ggcgatctgg cgccggtcgc gcaggctatt tcagcgacca atgatgaccc gttcagcgtg
      781 tgaaacaagt caccatggtc aagtctaggg cgccggatcc cgattttgtc gagacccaat
      841 agccatacgc gtcgacttca gcgcagggcc aaggctagga acttgaggca gaaatagaag
      901 gaagaggctt tgaaggaggg cggcttctat caggctacac cgggaaagca agccccaact
      961 gagcacatcc aagcgcctgc cccgcccacc ttgtcgagag tcgatcagcg ccatccaagg
     1021 tttcgcacag gacgcgccgg aatcgacgtc aggcgagcgc atccgcagcg ttgaccgtcg
     1081 gggcggacgc cgaggatgtc ggacgacgac gcaggaaata taggcccgcc aggatcgtgc
     1141 agacagcgcc gacgatgctg gtggcataga cgctggcgat gaagaagccg agccagtcca
     1201 ggttgaccat cgcgaagttg cgccagagca gcagggcgac gctgcccaga tagccagagg
     1261 cgtcggcaac atagatcagg aaacccgccg tgccgacctg accgctgaag gcgatcatgc
     1321 ggtcaaacag catggcgttg aagggcgtgt aggccatgta gaggccagcg ccggtcagga
     1381 tcatccaggt cagcggcgtc agcagatcag cctggaaggc cagggtcgac aggcccagca
     1441 acaggaagcc ggccaggatg acgccgtgca tcaccagaag cgcccgcaga ttgtcgcgca
     1501 ccagcatgac cgcgcccatt accgccaggg cgactgcggc gaccggcagt tcgctggtgg
     1561 tgaagacgcc agaggcctcg ccgaagccca gggcgatcca gatctcggcg gcgaaattat
     1621 cccggaagtc ccgcagtgcg gtcagcaaca cataggccat caccagcagc acgacgcccg
     1681 gcgcataggc ggccagaaag gcgcggcgcc gggcgcggtc cattggctgg cgacgcacgc
     1741 gcgcggcttc gtcggcgagg ctgggcggcg gcagctggac cagggcccag accgagaagg
     1801 ccagcagcgg cagaaacagc gcgccggtgg cggccggcat ccagaacggc gagacgtcca
     1861 gatcgaccat cagccacttt ccgaccgatt tgacgacgcc cgacgacagg atgaagctgg
     1921 cgcagaggat ggcgcccagc acctcggacg tgcgccgccc ctccatgaag ccgaacacca
     1981 gcccccagat catgcccagg ggcaggccgt tcagcaccag ggccacgaca ttccagggcg
     2041 cgggaacgac ggcgaaggcc agcagggcca gccaggacat accgatcaag cccaggatgg
     2101 ccacgccgcg atgggccggc gcaatctcgg aaatgacctt gaccccgatc agctttgagg
     2161 cggcgtagcc ggcgacctgg gccagcacga gggcgatctt atagtcgatg gcgaaggtcc
     2221 agccctcgac ctcgccgaag gtcgccgccg cgaacggctt gcggaaagcg tacatcgaga
     2281 aataggcgca aaagccggcc aggcccgcga acagggtgaa gaccagagga ttggctcgct
     2341 ccagccagag ccgcggacct gaactcatcg aaacgcccct tttgaggcct ttggtctata
     2401 ccactcgcat ggcgtttcga tgacgtggaa gggataccga aaagtggcgc ttcacagcct
     2461 tgggcgccca gcgacaggcg cgtgatttc
//
LOCUS       DAOMEO010000426         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4491_length_2489_cov_1.953985, whole genome shotgun sequence.
ACCESSION   DAOMEO010000426 DAOMEO010000000
VERSION     DAOMEO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_2489_cov_1.953985"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>389
                     /locus_tag="PLH31_10105"
     CDS             <1..>389
                     /locus_tag="PLH31_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HQR89591.1"
                     /translation="LADLALNAIEIVDRNLYERTCDVRWWATDSAVVSCVDAPSPETA
                     RHASQRLGVILDAYTVYLDLWICDARGRVVATGRPDRYPAAKSASVADAPWFRDAMKT
                     ATGDDFIACDIERVKALNDAPVATYAT"
     assembly_gap    390..399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <400..735
                     /locus_tag="PLH31_10110"
     CDS             <400..735
                     /locus_tag="PLH31_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HQR89592.1"
                     /translation="LGVHFDWKPQAQAVVDGVRLTTEERARSRVMLLDQKGRVLASSD
                     GRGVLTEVFPLNTAEGVMGSYADGDLTVGYALTPGYETYKGLGWYGCLAQTKEPASLV
                     ETMAKSAAA"
     gene            complement(645..>2093)
                     /locus_tag="PLH31_10115"
     CDS             complement(645..>2093)
                     /locus_tag="PLH31_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR89593.1"
                     /translation="RDIATAAGLRSCWSQPLHAADGKVLGSFAIYQRRVARPTPDDIG
                     FIEAAADLAAIAIERKRHEDDVALAQAQIRLALERERDAADAANKAKSEFLANVSHEL
                     RTPLNGVVGLIDTLEREALTAAQQDILDLIRQSGATLEHLVADLLDISRIDAGQLSLQ
                     PHAFDLVEAFDGLLKISHAQAQAKGLDFQVRYGPGASGRFLADSARIRQVLGNLLDNA
                     IKFTPQGRIDVAIDLNAGPDSSASRDLTLRVSDTGIGFGPETAARIFDRFNQADTTIT
                     RRFGGTGLGLSLCKALVELMGGAIAASSESGRGSLFTVTLPLAPDQAGLAPPSGQRAA
                     TNDLGSLRVLLAEDHPVNQRVVQLILAPHGADVVVVENGALALEAFKSGQFDLVLMDL
                     QMPVMDGLTATRAIRAHEEAGDFEKPCKRAPTCICPNPSPPWGWWRAFIRRWAPARPE
                     KSGQAAADFAIVSTREAGSLVWARQPYHPRPL"
     assembly_gap    2094..2103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2104..2461)
                     /locus_tag="PLH31_10120"
     CDS             complement(<2104..2461)
                     /locus_tag="PLH31_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQR89594.1"
                     /translation="MRNGGHVTGELKNTGKGNLFTVFGEPDIEIHDQPDGTITVEVKG
                     VDIYDPNTGEVRSSDVKGIAAWFIDTAYNEESFFVRHAYFLGANEPYKALKTALKAEI
                     DEDAWATLNKSTSRPFK"
BASE COUNT          393 a          810 c          833 g          433 t
ORIGIN      
        1 cctggccgac ctggcgctga acgccatcga gatcgtcgac cgcaatctct atgaacgcac
       61 ctgcgatgtt cgctggtggg ccaccgattc cgccgtggtc agctgcgtcg acgcgccatc
      121 gcccgagacg gcccggcacg ccagccaaag gctgggcgtg attctggacg cctataccgt
      181 ctatctcgac ctttggatct gcgatgcgag gggcagggtc gtcgcgacgg gccggcctga
      241 tcgctatcct gccgcaaaga gcgcctcggt cgctgacgcc ccctggttcc gcgacgccat
      301 gaagacggcc acaggcgacg acttcatcgc ctgcgacatc gagcgggtga aggccctgaa
      361 cgacgccccg gtcgccacct acgccacggn nnnnnnnnnc ttggcgtcca tttcgactgg
      421 aagccccagg cccaggcggt ggtcgacggc gtgcgcctga cgaccgagga acgcgcccgc
      481 tcgcgggtca tgctgctgga ccagaagggc cgcgtcctgg cctcatccga cgggcgcggc
      541 gtgctgaccg aagtctttcc gctcaacacg gccgaaggcg tcatgggcag ctatgcggac
      601 ggcgacctca cggtgggcta tgcgctgacg cctggttacg agacctataa gggcctgggg
      661 tggtatggct gtctggccca gaccaaggag ccggcctccc tcgtcgagac gatggcgaaa
      721 tccgccgccg cctgacctga tttctcaggc cgggcgggcg cccagcgcct gatgaatgcc
      781 cgccaccagc cccagggcgg tgatgggttt ggacagatgc aggtcggcgc ccgcttgcag
      841 ggcttctcga aatctccggc ttcctcgtgc gcccgaatgg cgcgcgtggc cgtcaggccg
      901 tccatcacgg gcatctgcag gtccatcagc accaggtcga actggccgct cttgaacgcc
      961 tccagcgcca gcgcgccgtt ctcgaccacg acgacgtccg cgccgtgtgg ggccaggatc
     1021 agctgcacca cgcgctgatt gaccggatga tcctcggcca gcaacacccg cagcgagccc
     1081 aggtcgttgg tcgcggctct ctggcctgac ggcggtgcga gtccggcctg gtccggcgcc
     1141 aggggcaggg tcaccgtgaa aaggctcccg cgccccgact ccgagctggc ggcaatcgcg
     1201 ccgcccatca gctccaccag cgccttgcac agtgacaggc caaggcccgt gccgccaaag
     1261 cgccgggtga tggtggtgtc ggcctggttg aagcggtcga agatccgggc ggctgtttcc
     1321 gggccaaagc caattccggt gtcggacacg cgcagggtca ggtcacgcga agcgctcgaa
     1381 tccggccccg cgttgagatc gatagcgacg tcgatccgac cttgcggggt aaacttgatc
     1441 gcattgtcca gcagattgcc cagcacctgc cggatgcggg cgctgtcggc caggaaccgg
     1501 cccgacgccc cggggccata gcggacctgg aaatcaagac ccttggcctg cgcctgggcg
     1561 tggctgatct tcagaagccc atcgaacgcc tcgaccagat caaaggcgtg gggctgcagg
     1621 ctcagctggc cggcgtcgat cctcgaaatg tccagcaggt cggcgaccag atgctccagg
     1681 gtcgcgcccg actggcggat caggtcgaga atgtcctgct gagcagccgt gagcgcctcg
     1741 cgctccaggg tgtcgatcag gccgaccacg ccgttcagcg gcgtgcgaag ctcgtggctg
     1801 acattggcga ggaactcgct cttggccttg ttggccgcat cggcggcgtc gcgttcgcgc
     1861 tccagcgcca ggcggatctg ggcctgggcc agggcgacgt cgtcctcgtg gcgctttcgc
     1921 tcgatggcga tcgccgccag gtcggcggcg gcctcgatga agccgatatc gtcgggcgtc
     1981 gggcgggcca cgcggcgttg atagatggcg aaactgccca gcaccttgcc atctgcggcg
     2041 tgaaggggct gcgaccagca ggagcgcaga ccggcggctg tcgcgatgtc tcgnnnnnnn
     2101 nnngcttgaa tgggcgtgag gtcgacttgt tcaaagtcgc ccaggcgtct tcgtcgatct
     2161 cggctttcag ggcggttttc agggccttgt agggctcgtt ggcgcccagg aagtaggcgt
     2221 ggcggacgaa gaaactttct tcgttatagg cggtgtcgat gaaccaggcg gcgatgccct
     2281 tgacgtcact ggagcgcacc tcgccggtgt tggggtcata gatgtcgacg cccttgacct
     2341 cgacggtgat ggtcccgtcc ggttggtcgt ggatctcgat gtcaggttcg ccgaacaccg
     2401 tgaacaggtt gcccttgccc gtgttcttca actcgcccgt gacgtgaccc ccatttctca
     2461 tccagcgata acgagagtcc tgggttgat
//
LOCUS       DAOMEO010000427         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4495_length_2487_cov_2.636102, whole genome shotgun sequence.
ACCESSION   DAOMEO010000427 DAOMEO010000000
VERSION     DAOMEO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_2487_cov_2.636102"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLH31_10125"
     CDS             <1..219
                     /locus_tag="PLH31_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HQR89595.1"
                     /translation="FGDLLVRGLMEKRRENPETFAPLYEELLAAGGTKTYVEALKPFG
                     LNPREKAFWAAGCAQLERLVDEFEALIT"
     gene            219..1601
                     /locus_tag="PLH31_10130"
     CDS             219..1601
                     /locus_tag="PLH31_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HQR89596.1"
                     /translation="MADDIRDPERDRFSGRLSRFAKVGAGLSGAAVSYGANSLFGGDS
                     ADARNAKALRAALGGLKGPLMKAAQMFATVPDLLPPEFAAELAELQTNAPAMGWPFVR
                     RRMAAELGPDWQSKFQSFEHEAAHAASLGQVHRAVAPDGRAIAVKLQYPDMQSAVESD
                     LGQLRSLFALFKRMDGSIDPTQMIEEIGDRLREELDYGREAKLMALYGGFFSGRPEIR
                     VPEPLASLSTGRLLSMTWMDGQGLLAFKDAPQETRDRIAALLFEAWWSPMTKLGIIHG
                     DPHLGNYTFGGAEGGPNGWQEAAHLNLLDFGCIRIFPPRFVAGVVRLYRALSNDDRAE
                     QYEAYRSWGFANLNDDLVDTLNVWARFIYGPLLDDRVRTVADDVPPGEYGRREAFKVR
                     QALKEKGPITIPREFVFMDRAAIGLGAAFLHLGARHNWKALFEASLEGFSEEGLAQRQ
                     AAVLAAAGVA"
     gene            complement(1642..2226)
                     /locus_tag="PLH31_10135"
     CDS             complement(1642..2226)
                     /locus_tag="PLH31_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89597.1"
                     /translation="MEFDRLEQAWRSGANTPTDEVQARLKEQLMHTLKTRRRTELLLT
                     GVPAATLTLFSVIALAVVQRNEGAGWPGLAMLALCWGVMGAVLWNSLRHRSRDTGSPL
                     RDTVAGLLAANRRARSNCHVFWAMLPVFMVPLFLALQRLQQDGRMTTAEGWQALGVCG
                     VALAASAGWNALRYVMVLKPEQKRLEALLAEYNG"
     gene            complement(2216..>2487)
                     /locus_tag="PLH31_10140"
     CDS             complement(2216..>2487)
                     /locus_tag="PLH31_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HQR89598.1"
                     /translation="RRGEAIEAEMALRLQQDGAEAEAGLLERLYAAIRTLPPVERSLI
                     LLSLDGVAYAEIARLHGLSETNVGARLTRIRARLSTLVKDDGDGV"
BASE COUNT          399 a          834 c          854 g          400 t
ORIGIN      
        1 ttcggcgacc tgctggtgcg cggcctaatg gagaagcgcc gcgaaaatcc cgagaccttt
       61 gcgccgctct atgaagagct gctcgcggcc ggcgggacca agacctatgt cgaggcgctg
      121 aagccgtttg gcctcaaccc gcgtgaaaaa gccttctggg cggcgggctg cgcgcaactg
      181 gagcggctgg tcgatgaatt cgaggctttg atcacctgat ggccgacgat atccgcgacc
      241 ccgaacgcga ccgcttttcg ggacgcctga gccgcttcgc caaggtgggc gcggggcttt
      301 ccggcgcagc cgtcagctac ggcgccaaca gcctgtttgg cggcgacagc gccgacgccc
      361 gcaacgccaa ggccctgcgc gcagcgctgg gcggactgaa agggccgctg atgaaggcgg
      421 cccagatgtt cgccacggtg ccggacctgc tgccgccgga attcgcggcc gagctggccg
      481 agctgcagac caacgccccg gccatgggtt ggcccttcgt ccgtcgtcgg atggctgccg
      541 agctggggcc cgactggcaa tccaagttcc agagtttcga gcacgaggcc gcccatgccg
      601 cctccctggg tcaggtgcac cgcgccgtcg cccccgatgg ccgggcgatc gccgtgaagc
      661 tgcaatatcc cgacatgcag agcgccgtcg aaagcgacct tggccagctg cgctcgctgt
      721 tcgccctctt caagcgcatg gacggctcga tcgacccgac ccagatgatc gaggagatcg
      781 gcgaccggct gcgcgaggag ctggattacg gccgcgaagc caagctgatg gcgctgtatg
      841 gtggcttctt ctccggccgc ccggaaattc gggtgcccga gcccttggcc tcgctgtcga
      901 cgggtcgact gctcagcatg acctggatgg atggccaggg cctgctggcc ttcaaggatg
      961 cgccccagga gacccgcgac cgcatcgccg ccctgctatt cgaagcctgg tggagcccga
     1021 tgaccaagct gggcatcatc catggcgacc cgcacctggg caattacacc ttcggcgggg
     1081 ccgagggcgg ccccaatggc tggcaagagg ccgcgcacct gaacctgctg gacttcggct
     1141 gtatccgaat cttcccgccg cgcttcgtgg ccggtgtggt gcggctctat cgcgcgctgt
     1201 cgaatgacga ccgcgccgaa cagtatgagg cctatcgcag ctggggcttc gccaatctca
     1261 atgatgatct ggtcgacacg ctgaacgtct gggcccggtt catctatggg ccgctgctgg
     1321 acgatcgcgt ccggacggtc gccgatgatg tgccacccgg ggaatacggc cggcgcgagg
     1381 ccttcaaggt gcgtcaggcc ctgaaggaga agggaccgat caccatcccc cgggaatttg
     1441 tgttcatgga ccgcgccgcc atcggcctgg gcgcggcctt cctgcatctg ggcgcgcgcc
     1501 acaactggaa ggcgctgttc gaggcctcgc tggaagggtt ttccgaagag ggtctggccc
     1561 agcgacaggc ggcggtgctg gcggcggcgg gcgtcgcctg acgctggaat acgggatcaa
     1621 cctgcgcgcc ggcggagcct tctagccgtt gtattcggcc agcagcgctt ccagtcgctt
     1681 ctgctcgggc ttgaggacca tgacgtagcg cagggcgttc cagcccgcgc tggccgccag
     1741 cgccacaccg caaacgccca gggcctgcca gccttcggcc gtcgtcatcc gcccgtcctg
     1801 ctggagccgc tggagggcca ggaacagcgg aaccatgaac actggcagca tggcccagaa
     1861 gacatggcaa ttggaccgcg cccggcggtt cgcggccaga aggccggcca cggtgtcgcg
     1921 caggggcgag cccgtgtcgc gggaccggtg gcggaggctg ttccagagaa ccgcgcccat
     1981 caccccccag cagagggcca gcatggccag tcccggccat cccgcgccct cgtttcgctg
     2041 gacgacggcc agggcgatga cactgaacag ggtcagggtc gcggccggga cgccggtcag
     2101 tagcagttcc gtgcggcgac gggttttcag ggtgtgcatc agctgctcct tcaagcgggc
     2161 ttggacttca tcggtcgggg tgttggcgcc ggaacgccag gcctgttcga gacggtcaaa
     2221 ctccatcgcc gtcatccttc acgagggttg agagccgggc gcgaatccgg gtgaggcgcg
     2281 caccgacatt ggtttccgaa agcccgtgca gccgggcgat ctcggcatag gcgacgccgt
     2341 ccagcgacag caggatcagc gagcgctcca ccggcggcag ggtgcggatc gccgcataga
     2401 gccgctccag caggccggcc tcggcctcgg cgccatcctg ctgcaggcgc agggccatct
     2461 cggcctcgat cgcttcgcct cgccggc
//
LOCUS       DAOMEO010000428         2487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4496_length_2487_cov_2.170230, whole genome shotgun sequence.
ACCESSION   DAOMEO010000428 DAOMEO010000000
VERSION     DAOMEO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_2487_cov_2.170230"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..525
                     /locus_tag="PLH31_10145"
     CDS             214..525
                     /locus_tag="PLH31_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89599.1"
                     /translation="MKYPIAFAAGIAGSLFIAQAASAAGGFEDQMGRCLQQYANTRDA
                     AQVMLECTADAGKLSACKVVDNSAAGKGFDKAAMCIAEKLPMGAKTGTVKVPFRFPGG
                     A"
     gene            complement(724..2307)
                     /locus_tag="PLH31_10150"
     CDS             complement(724..2307)
                     /locus_tag="PLH31_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR89600.1"
                     /translation="MSQDHDVADRVAFIGLDDRARQALRDLRPLIQKEINPALAAFYG
                     KVKTTPETSKFFRDERHMDAASSRQAAHWDVIAQAEFSDTYVNTVRTIGEVHARIGLE
                     PRWYIGGYAMVMDRLLRATVDSLWPKGFMAKGDPAQAGEAISALMKAVMLDMDFAISI
                     YLETIDAERQRLEAARVENEQCQTRLVESLAEALAHLAQGDLVSRLDDEVKPQFQKLK
                     DDFNSAVGTLEQAMSRVSGVTGNIRAGSDEIGNAADDMSRRTEQQAASLEQTAAALDQ
                     ITSTVRRSAAGAKQASEVVAGAKTEAERSGVVVDQAVAAMGEIEGSSREIGNIIGVID
                     EIAFQTNLLALNAGVEAARAGEAGRGFAVVAQEVRALAQRSADAAKEIKTLISSSTLQ
                     VSQGVSLVGQTGDALRGIVAKVAEIDTLIVQISASAQEQATGLHQVNTAVNQMDQVTQ
                     QNAAMVEQTTAATHSLKDQTAELVNLISAFRVSGSRPVASAPAARKPASPVSRPGTGS
                     RPVARGSAALAVREEWEEF"
BASE COUNT          428 a          782 c          779 g          498 t
ORIGIN      
        1 aagcgtctcc tttcgggaca attggtcgca catgaatcta taaagggcgc acaggtcggg
       61 tccgggctcc gaggtgtaga cagggttacc gcaaagtaaa ttttgaatat tcttcgccga
      121 ctcagaatat cttaactcgc agctgcgaaa aacgtcctgc gtctggttgg cgcgtcgtcc
      181 acaggacgtg tattcattat taggagcagc gggatgaaat atccgatcgc ttttgccgcg
      241 ggaatcgctg gcagcttgtt catcgcccag gccgcctcgg ccgctggcgg tttcgaagac
      301 caaatgggcc gttgcctgca acaatacgcc aacacccgtg acgccgctca ggtgatgctt
      361 gagtgcaccg ctgacgccgg caagctctcg gcttgcaagg tcgttgacaa cagcgccgcc
      421 ggcaagggct tcgacaaggc cgccatgtgc atcgctgaaa agctgccgat gggcgccaag
      481 accggcaccg tgaaggtccc gttccgcttc ccgggcggcg cgtaaaccgc caggctccgt
      541 attcggggtt caaaaacgcc tccgctgacc agaccaaacg ttcccatgga atgtttcggt
      601 ctggtcttcg ggggcgtttt gctttgcgcc accaaccttt gaaaaagcgg gcgtcggtaa
      661 ggacgcccgc tatagtcgac atgcaaggcg caagcccgct cggtctatcg ggaccgtctt
      721 tccctagaac tcttcccact cctcgcggac cgccagagcg gcggagcccc gcgcgacagg
      781 gcgcgaaccg gttcctggtc ggctgaccgg cgaggcgggt ttgcgggcgg ctggcgctga
      841 agcgacgggc cgagagccgc tgacgcggaa ggcggagatc aggttgacca gctctgcagt
      901 ctgatccttg agcgagtggg tcgccgccgt cgtctgttcg accatggcgg cgttctgctg
      961 ggtcacctga tccatctgat tgaccgcagt attgacctgg tgcaggccgg tggcctgttc
     1021 ctgggccgag gcggagatct ggacgatcag ggtgtcgatc tccgccacct tggcgacgat
     1081 cccacgcagg gcgtcgccgg tttgtccgac gaggctgacc ccctggctga cctgcagcgt
     1141 cgaggacgag atcagggtct tgatctcctt ggccgcatcg gcggaacgct gagccagggc
     1201 tcgcacttcc tgggccacga cagcaaagcc acgaccggcc tcgcccgctc gcgccgcttc
     1261 gacgccggcg ttaagcgcca gaagattggt ctggaaggcg atctcatcga tgacgccgat
     1321 gatgttgccg atttcgcgcg aagagccttc gatctcgccc atggcggcca cggcctgatc
     1381 cacgacaacg cccgatcgtt cggcttcggt cttggccccg gccacgacct cggaggcctg
     1441 tttggcgccc gccgcagagc ggcgtacggt ggaggttatc tgatccaggg cggcggccgt
     1501 ctgttccagg ctggcggcct gctgctcggt gcggcgcgac atgtcgtcgg cggcgttgcc
     1561 gatctcgtca gagcccgcgc gaatattgcc cgtcacaccc gaaacgcgac tcatggcctg
     1621 ttccagagtg ccgaccgcgc tattgaagtc gtctttcagt ttctggaact ggggcttgac
     1681 ctcgtcgtcg aggcggctga ccaggtctcc ctgcgccaga tgggcgagcg cttcggccag
     1741 gctttcaaca agcctggtct ggcattgctc gttctcgaca cgcgcggcct cgagtcgctg
     1801 gcgctcggca tcgatggttt cgagatagat cgagatggcg aagtccatgt cgagcatcac
     1861 cgccttcatc agggcgctga tcgcttcgcc ggcctgggca gggtcgccct tggccatgaa
     1921 acccttgggc cagagcgaat cgacggtggc gcgcagcagc cggtccatga ccatggcgta
     1981 gccgccaatg taccagcgcg gctccaggcc gatccgcgcg tgaacttccc cgatggtccg
     2041 gaccgtattg acataggtgt cgctgaactc ggcctgggcg atcacatccc agtgcgccgc
     2101 ctggcgactg ctggcggcgt ccatgtgccg ctcgtccctg aagaatttgc tggtctccgg
     2161 cgtggtcttg accttgccgt agaaggcggc cagcgcgggg ttgatctcct tctggatcag
     2221 cggccgaaga tcacgcagag cctgtcgcgc gcgatcatcg agtcctatga acgcaacacg
     2281 atcggctaca tcatgatcct ggctcatcag ctttgtccgt cccggagttt aagttaccga
     2341 acggaagcct ctgccagtat gcttaatccg accttaaata ttcgcgcctg agccgcgtca
     2401 tgtcgccaca ctcgccgaaa agtcgcagtt cgccgaacgg ggctatccgc cctgcttcat
     2461 ggtctcgcgg gcgatgatca cctgctg
//
LOCUS       DAOMEO010000429         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4503_length_2482_cov_2.843840, whole genome shotgun sequence.
ACCESSION   DAOMEO010000429 DAOMEO010000000
VERSION     DAOMEO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_2482_cov_2.843840"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1894)
                     /locus_tag="PLH31_10155"
     CDS             complement(116..1894)
                     /locus_tag="PLH31_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQR89601.1"
                     /translation="MAQPPQRPKAPWDEAFLPPAPQWQGASQALLRDRSDPWVTAFEA
                     DPEHDFSPNYVDTRKWFDRLDKASSLIRIEDFGVSPEGRPIYAVIASKDGAKLDPGKP
                     LLLAQAGIHPGEIDGKDAGMMLLRDMAFNGKDGLLDKVNLVLIPILSVDGHERASAYS
                     RPNQRGPRIQGWRNTATNQNLNRDFMKLDQPEMQALKALQARYRPDLYVDIHVTDGMD
                     YQYDVTYGYNGENGVWSRSPAIAKWLDATFKPAMNQGLEAQGHIPGELVFGIDEHGPK
                     AGLSDGGLGERYSNGWGSAAHIPTILIENHSLKPHAQRVLGTYVFLETAMKLLAEQGD
                     SLRAAIDQDRALRPVEIPTNFVAEDKPSATRTFKGILYETYQSPASGRTEIRWLGQPD
                     PQVWSLPFYGSRPTLTLKRPAAYWVPSYRKDLIERLKLHGLTIEPLAAAKTLQVEMLR
                     LTDPKIAARANEGHVQISVSQVAPETRDWTFPAGSVRVPTDQPLGDLAVLLLEPQSSE
                     SFFAWGMVPEVLSRVEYIEGYAIAPLAEKMMAADPALKAEFEAKLAADPKFAADPEAR
                     LGWFYRRTPFYDDRHLLYPIARELAP"
     gene            complement(1969..>2482)
                     /locus_tag="PLH31_10160"
     CDS             complement(1969..>2482)
                     /locus_tag="PLH31_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89602.1"
                     /translation="EVACNQGERAMSEFPVVYDLESNVVRIDGAGGATVLLNMVHAAK
                     FGAPLNPDLIFNPGVAALLTGLKAASLRPEPLWATPFTQADIVAFAGLVLEKAGELGW
                     WHMDHTEQVSLLQNVVAAPHRFSSAQIEMIQAEAIGQLNRMRDIIEAVPPLSEEDREW
                     LEANLTDDNW"
BASE COUNT          395 a          763 c          846 g          478 t
ORIGIN      
        1 ggcggcgtga cccgaagtta tcgtatccat cggtgaaccc tacgaattta accgcgcccg
       61 gcgcgggagg ggcgcataaa agccctctcg cgcccgcaca tgcaagcgat tggccctatg
      121 gggcgagttc ccgggcgatc ggatacagca ggtggcggtc gtcgtagaag ggcgtgcggc
      181 ggtagaacca gccaagccgc gcctccggat cggcggcgaa cttcgggtcg gcggccagct
      241 tggcctcgaa ctcggccttc agcgcagggt ccgccgccat catcttctcg gctagcggcg
      301 cgatggcgta gccctcgata tattcgacgc ggctcagcac ctctgggacc atgccccagg
      361 caaagaagct ctcgcttgac tgcggctcca gcagcagcac ggccaggtcg cccagcggtt
      421 ggtcggtggg tacgcgcacc gagccggcgg ggaaggtcca gtcgcgggtc tccggcgcga
      481 cctggctgac gctgatctgc acgtggcctt cattggctcg cgcggcgatc ttggggtcgg
      541 tcaggcgcag catttcgacc tgcaaggtct tggcggcggc cagaggctcg atcgtcaggc
      601 catgcagttt cagccgctcg atcaggtcct tgcgatagct gggcacccag taggcggcgg
      661 ggcgcttcag ggtcagggtc gggcgcgagc cgtagaaggg cagggaccag acctgcggat
      721 cgggctggcc cagccagcgg atctcggtgc ggccggaggc cgggctctga taggtctcgt
      781 agagtatgcc cttgaaggtg cgggtggcgc tgggcttgtc ctcggcgacg aaattggtcg
      841 ggatctctac ggggcgcagg gcgcgatcct ggtcgatggc ggcgcgcagg ctgtcgccct
      901 gctctgccag cagtttcatc gccgtctcga ggaacacata ggttccgagc acgcgctggg
      961 cgtggggctt gaggctgtgg ttctcgatca ggatggtcgg gatgtgggcc gccgagcccc
     1021 agccgttcga gtaccgctcg cccaggccgc cgtccgacag gccagccttg ggcccgtgct
     1081 cgtcgatgcc gaacaccagc tcgccgggga tgtgaccctg cgcctccagg ccctggttca
     1141 tggccggctt gaaggtcgcg tcaagccact tggcgatggc cggtgagcgc gaccagacac
     1201 cgttctcgcc gttgtagccg taggtgacgt cgtactggta atccatgccg tcggtgacgt
     1261 ggatgtcgac atagaggtct ggacggtacc gggcctgcag cgctttcagc gcctgcatct
     1321 ccggctggtc cagcttcatg aagtcgcgat tgaggttctg gttggtggcg gtgttgcgcc
     1381 agccctggat gcgcggcccg cgctggttgg ggcggctata ggcgctggcc cgctcgtgtc
     1441 catcgacgct gaggatcggg atcaggacca ggttgacctt gtcgagcagg ccatccttgc
     1501 cgttgaaggc catgtcgcgc agcagcatca tgcccgcgtc cttgccgtcg atctcgcccg
     1561 gatggatgcc ggcctgggcc agcagcaagg gcttcccggg atcgagcttg gcgccgtctt
     1621 tcgacgcgat caccgcatag atgggccggc cctctggcga gacgccgaag tcctcgatgc
     1681 ggatcaggct ggaggccttg tccagccggt cgaaccactt gcgggtgtcg acatagttcg
     1741 ggctgaagtc gtgctcggga tccgcctcga acgctgtcac ccacggatcg gatctgtcgc
     1801 gcagcaatgc ctggctggcg ccctgccact gtggcgcggg cggcaggaag gcttcgtccc
     1861 aaggggcttt ggggcgttga gggggttgag ccatgctcgt actcgctgtc agggcggcga
     1921 ccagggtcgc tagggtgagg atccggtatt tcattgcctg tccgcagctc accagttgtc
     1981 gtcagtcaaa ttcgcttcca gccactcgcg atcctcttca gagagcggcg gtacagcttc
     2041 aatgatgtct cgcattcgat tgagttgccc gatggcttct gcctggatca tctcgatttg
     2101 cgcagacgaa aatcggtgcg gggccgcgac gacgttctgc aagagggaga cctgttctgt
     2161 gtggtccatg tgccaccagc ccagctcccc agctttctcg agcacaaggc ctgcaaaggc
     2221 gacaatatcc gcctgcgtga acggtgtcgc ccacaagggt tcaggcctca ggctggctgc
     2281 cttaagcccc gtgagcagag ccgctacccc agggttgaag atgagatcag ggttgagcgg
     2341 tgctccgaac ttagccgcat ggaccatgtt cagcagcaca gtggcaccgc ccgcgccgtc
     2401 tatgcgaacg acgttgcttt caagatcata aacaaccggg aactctgaca tcgcgcgctc
     2461 tccctggttg caagctactt cc
//
LOCUS       DAOMEO010000430         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4504_length_2482_cov_2.652658, whole genome shotgun sequence.
ACCESSION   DAOMEO010000430 DAOMEO010000000
VERSION     DAOMEO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_2482_cov_2.652658"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLH31_10165"
     CDS             <1..310
                     /locus_tag="PLH31_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89603.1"
                     /translation="AASVNDAHAVLARFLFRNSAAHQMAGTLSGGERLRAALACVLCA
                     ATPPQLLILDEPTNHLDLASIQAIEAALAGYDGALLAVSHDADFLDAIGVQRRVELGT
                     "
     gene            395..1684
                     /locus_tag="PLH31_10170"
     CDS             395..1684
                     /locus_tag="PLH31_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HQR89604.1"
                     /translation="MTKTTAQAATQAAGDHPLRVTLSELMHLRSLPLERAPVRLRQWL
                     MLVAPAEREAEATFLGLLHPDRSDAIATGRLLVPTEQGGWLWERHQEFSTWTAYDYRP
                     LGERLGFEPRDEAAFPWLEGAPGAVFRGVEITVSAASDAAPDLAAIGRSIDLARCVSC
                     EVFDGAARIWTDFRLGEGGAGRIHVHNRGLANDEVSRLVQTLLEIGNYRKLALMGFPP
                     ARELLGWLDGAEARLAAITGELALAGGDSQSVLDQLLALSAEVELRAASTRFRRGATE
                     SYHQLTLDRLEALREVRVSGHSTMREFIARRLLPAMRTCEAADRRLDDLSARIGRSSD
                     LLRAKLGMALDRQNQALLHSMNERVALQTKLQGLVEGLSVFAVSYYVVGLAGYLLKPW
                     LHDLPGAAETALSLLVPLVLAAVTVGLHRRKKRIVGS"
     gene            complement(1681..2154)
                     /locus_tag="PLH31_10175"
     CDS             complement(1681..2154)
                     /locus_tag="PLH31_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HQR89605.1"
                     /translation="MSFTISGLPVEDFKPLFDLDEDALAARGIIRRVATAKPGFPCRI
                     GLRDAELGETVLLLNHEHQSADTPYRSSYAIYVSQAAVETWSERDTIPSAMRGRPISL
                     RAFSPEGMLVGAEVAMGDALEAAIARQLAAPNAAYLHAHNAGHGCFVARIDRADA"
     gene            complement(2249..>2482)
                     /locus_tag="PLH31_10180"
     CDS             complement(2249..>2482)
                     /locus_tag="PLH31_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HQR89606.1"
                     /translation="ADAALRQRLIRATEAFDAVLARQARALNAARTVTEGLVKAVAEE
                     IANQRAPASGYGAGARMNNPVSSGTAITLNKKA"
BASE COUNT          341 a          827 c          935 g          379 t
ORIGIN      
        1 ggcggcttcg gtcaatgacg cccatgccgt gctggcgcgg ttcctgtttc gcaatagcgc
       61 cgcccaccag atggccggaa ccctgagcgg cggcgaacgc ctgcgcgcgg ccctggcctg
      121 cgtgctgtgc gcggcgacgc cgccccaatt gctgatcctg gacgaaccca ccaaccatct
      181 cgacctggcc tccatccagg ccatagaggc ggcgctggcg ggctatgacg gcgcgctgct
      241 ggctgtcagc cacgacgctg atttcctcga cgccatcggg gtgcagcggc gggtcgaact
      301 cgggacatga cggcgtggac gtctcgcgct agtctctcat gtgaccggta acattgcgcc
      361 tgatgcgatg acccggcgat gcgggacgag gacgatgacc aagacgacgg cccaggcagc
      421 gacccaagcg gcaggcgacc atccgctgcg ggtgacgctg agcgagctga tgcacctgcg
      481 gtccctgccg ctggagcgcg cgccggtgcg gctgcggcaa tggctgatgc tggtcgcgcc
      541 cgccgagcgc gaggccgagg cgacgttcct gggtctgctg caccccgacc ggtccgacgc
      601 catcgcgacc gggcgcctgt tggtcccgac cgagcagggc ggctggctgt gggagcggca
      661 tcaggagttt tcgacctgga ccgcctacga ctacaggccg ttgggcgagc gcctgggctt
      721 cgagccgcgt gacgaggcgg ccttcccttg gcttgagggc gcgccggggg cggtgttccg
      781 gggcgtggag atcacggtct cggcggcgag cgacgccgcg ccggacctgg cggcgatcgg
      841 gcgcagcatc gacctggccc gctgcgtcag ctgcgaggtc ttcgacggcg cggcgcggat
      901 ctggaccgac tttcggctgg gcgagggcgg ggcggggcgc atccatgtgc acaatcgcgg
      961 cctggccaat gacgaggtct cgcgcctggt ccagaccctg ctggagatcg gcaactatcg
     1021 gaagctggcc ttgatgggct ttccgcccgc ccgtgaactg ctgggctggc tcgacggggc
     1081 cgaggcgcgg ctggcggcga tcaccggcga gctggccctg gcgggcggcg acagccagtc
     1141 ggtgctggac cagcttctgg ccctatcggc cgaggtggag ctgcgcgcgg ccagcacccg
     1201 cttccggcgc ggggcgaccg aatcctatca ccagctgacg ctcgaccggc tggaggccct
     1261 gcgcgaggtg cgggtgtcgg gccattcgac catgcgcgag ttcatcgccc gccgcctgct
     1321 gccggccatg cgcacctgcg aggccgccga ccggcggctg gacgacctgt cggcgcggat
     1381 cggccgctcc agcgacctgc tgcgcgccaa gctgggcatg gcgctggacc gccagaacca
     1441 ggccctgctg cacagcatga acgagcgggt ggccctgcag accaagctgc agggactggt
     1501 cgagggcctg tcggtgttcg ccgtcagcta ctacgtggtc ggcctggcgg gctatctgct
     1561 caagccctgg ctgcatgatc tgcctggggc ggccgagacg gcgctgtcgc tgctggtccc
     1621 gctggtgctg gccgccgtca ccgtcggcct gcaccgtcgc aagaagcgga tcgtggggag
     1681 ctaggcgtcg gcccggtcga tgcgggccac gaagcagcca tggccggcat tgtgggcgtg
     1741 cagataggcg gcgttgggag ccgccagctg ccgggcgatg gcggcttcca gcgcatcgcc
     1801 catcgccacc tcggcgccca ccagcatgcc ctcagggctg aaggcgcgca gagagatcgg
     1861 ccggccgcgc atggcggacg ggatggtgtc gcgttcgctc caggtctcga cggcggcctg
     1921 gctgacatag atggcgtagg acgagcgata gggcgtgtcg gccgattgat gctcgtggtt
     1981 cagcagcagc acggtctcgc ccagctcggc gtcgcgcagg ccgatgcggc agggaaagcc
     2041 cggcttggcg gtggcgacgc ggcggatgat cccccgcgcg gccagggcgt cctcatcgag
     2101 gtcgaacagg ggcttgaagt cttcgaccgg caggccggaa atcgtgaagg acatgggacg
     2161 tctccagaag agggctgagg caggggcccc cttcatgcgt cgcccgcgcc accggcagcc
     2221 acccgatgct tgcgatcgga tgaggccgct aggccttctt gttcagcgtg atcgccgtgc
     2281 cgctggacac cggattattc atccgcgcgc cggcgccgta gcccgaggcc ggagcgcgct
     2341 ggttggcgat ctcttcggcc acggccttga ccaggccttc ggtcacggtg cgggcggcgt
     2401 tgagggcgcg cgcctggcga gcgagcaccg cgtcgaacgc ttcggtggcg cggatcaggc
     2461 gctggcgcag ggcggcgtcg gc
//
LOCUS       DAOMEO010000431         2478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4516_length_2478_cov_2.556748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000431 DAOMEO010000000
VERSION     DAOMEO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4516_length_2478_cov_2.556748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLH31_10185"
     CDS             <1..477
                     /locus_tag="PLH31_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQR89607.1"
                     /translation="IRSLEKTAMEPDKIRRLIEVLDDNHGYALYRSVSGLKEALSAHE
                     RATFRFEAGSILIEQEVERSTFEGWIAPELAAIETAVDQALARANLAPDQVDRVFLTG
                     GSSFVPAVRDIFQRRFAADRIESGGEFESIASGLALIGRESNLDLWTMRAAEKSPA"
     gene            591..>1973
                     /locus_tag="PLH31_10190"
     CDS             591..>1973
                     /locus_tag="PLH31_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice nucleation protein"
                     /protein_id="HQR89608.1"
                     /translation="MPISYLSAAQIAALPTTIIDDLSTTKLATLSSTQVGAISATAVA
                     VLDSTQVEALSATQVKGLSATQIPSFGAGVAFNAQQLAAFSGELVGAMTSTQLASLDA
                     AKLDVLGSTQIAAISATNFSGLNATQVASLDVTQLKGLTATQMAGLTTTDIGELAASQ
                     IGGLAASQIGSLEAAAIAAFAAAQLAALSTTQIGGMTASHIAALSATQLGALEATQIG
                     ALSAAQVAGLSGTQVSTLSDTQVGTMNATLLGGLSETALSTLTATQMASFSPAQIGGL
                     TTTQIATLTATDFAELSATQVGGLTASQLGALSTTNLNALTGAQIGALTSTQFAGLTA
                     TQLGGLGSGDFAELSMTQIANLTASQVGGISASNISSFNATQVQGLSATQLGGLTSTQ
                     LGGFSTTDIGEFSATQIGGLTASQIGSLSVTNLTALDTTQIGAISPTAMRGLSAYQVR
                     SLTLDDFNGLN"
     assembly_gap    1974..1983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1984..2187
                     /locus_tag="PLH31_10195"
                     /pseudo
     CDS             1984..2187
                     /locus_tag="PLH31_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516915.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ice nucleation protein"
     gene            complement(2159..>2478)
                     /locus_tag="PLH31_10200"
     CDS             complement(2159..>2478)
                     /locus_tag="PLH31_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR89609.1"
                     /translation="VNAAGEEASVRPAGPLRVNNADALMPALIAGQGLAVLPDFIAGE
                     ALVDGRLEAVMPDWTPPPIALHLVMPPGGPRPARVEALTAFLTSALGARARGSTGYPP
                     GPA"
BASE COUNT          430 a          928 c          737 g          373 t
ORIGIN      
        1 atccgctcgc tggaaaagac cgcgatggag ccggacaaga tccgccgcct gatcgaggtg
       61 ctggacgaca atcacggcta cgccctttac cgctccgttt cgggcctgaa ggaagcgctg
      121 tcggcccatg agcgcgccac gttccgcttc gaggccggct cgatcctgat cgagcaggag
      181 gtcgaacgct ccaccttcga gggctggatc gcgccggaac tggccgcgat cgagacggcc
      241 gttgatcagg ccctggcccg ggccaacctg gcgcccgacc aggtggaccg cgtattcctg
      301 accggcggca gctcgttcgt gccggccgtg cgcgacatct tccagcgccg cttcgcagcc
      361 gaccggatcg agagcggcgg ggagttcgag tcgatcgcct cgggcctggc cctgatcggc
      421 cgcgagagta atctggacct ttggaccatg cgagcggctg aaaagtctcc ggcctgaaca
      481 gtggcagact gccggccaat caacgactta cctgctctac tttgtcacgt agaccgaatc
      541 tcgcattgca cctagcctga ctgttaacca ttttggtcag gacgagccct ttgccgatct
      601 catatctctc ggccgcgcag atcgccgcgc taccgacgac gatcattgat gatctgtcga
      661 ccaccaagct cgccaccctc agcagcaccc aggtcggcgc gatcagcgcc acggctgtcg
      721 ctgttctcga ttccacacaa gttgaagccc tgtcggccac ccaggtcaaa ggcctctcgg
      781 cgacccagat ccccagcttc ggcgcgggcg tggcgttcaa cgcccagcaa ctggcggcct
      841 tcagcggcga gctggtcgga gccatgacct cgacccagct ggccagcctc gacgccgcca
      901 agctggacgt gctgggctcc acccagatcg ccgcgatctc ggcgaccaat ttctcgggcc
      961 tcaacgccac ccaggtggcc tcgctggacg tgacccagtt gaaggggctc accgccaccc
     1021 agatggcggg cctgacgacg acggatatcg gcgagctggc cgccagccag atcgggggtc
     1081 ttgcggccag ccagatcggc agcctggaag ccgccgcgat cgccgccttc gccgccgccc
     1141 agctggccgc cctgtcgacg acccagatcg gcggcatgac cgccagccac atcgccgcct
     1201 tgagcgccac gcagctgggc gccctggaag ccacccagat cggcgccctt tcggccgccc
     1261 aggtcgccgg cttgagcgga acccaggtct caaccctgtc cgacacccag gtcggcacga
     1321 tgaacgccac cctgttgggc ggcctcagcg aaaccgccct ctcgaccctg accgctacac
     1381 agatggccag cttcagcccc gcccagatcg gcggcctgac caccacccag atcgcgaccc
     1441 tgaccgccac cgacttcgcc gagctatcgg ccacccaggt cggcggcctg accgcctcgc
     1501 agctgggcgc gttgtcgacc accaacctca acgcgttgac gggcgcccag atcggagcgc
     1561 tcaccagcac ccagttcgcc ggcctgaccg cgacacagct gggtggcctg ggctctggtg
     1621 acttcgccga actgtcgatg acccagatcg ccaacctgac ggccagccag gtcggcggca
     1681 tctcggcgag caatatctcc agcttcaacg ccacccaggt ccagggcctg tcggcaactc
     1741 agctgggcgg tctcacctcc acccagctcg gcggcttcag cacaaccgat atcggcgagt
     1801 tctcggccac gcagattggc ggactgacag cgtcgcagat cggatccctg tcggtgacca
     1861 acctgacggc gctggacacc acccagatcg gcgcgatcag cccgaccgcc atgcgcggcc
     1921 tgtcggccta tcaggtgcgc agcctgacgc tcgacgattt caacggcctg aacnnnnnnn
     1981 nnnggcgccc taacggccac ccaggtctcg gccctgtcca cgaccgtgat cggcggtctg
     2041 accaccaccc aggtcggcta cctgacgccg acccagatcc ccggcctgac catcacccag
     2101 ctggactggc tttcgaccac caatatcgcc gcgatgagcc cgctacaggt gggagccttc
     2161 acgccggccc aggtggatag cctgtagaac cccgcgccct ggcgcctagc gccgaggtca
     2221 ggaacgccgt cagcgcctcg acccgcgccg gcctgggacc accgggcggc atcaccaaat
     2281 gcagggcgat cggcggcggg gtccagtcag gcatcaccgc ttccagccgg ccatcgacca
     2341 gggcctctcc cgcgataaag tcaggcaata cggccagccc ttgccccgcg atcagggccg
     2401 gcatcagggc gtcggcgtta ttgacccgca gcggccccgc cgggcgcacc gaagcctcct
     2461 cccccgccgc attgacga
//
LOCUS       DAOMEO010000432         2476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4521_length_2476_cov_2.481206, whole genome shotgun sequence.
ACCESSION   DAOMEO010000432 DAOMEO010000000
VERSION     DAOMEO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_2476_cov_2.481206"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="hemA"
                     /locus_tag="PLH31_10205"
     CDS             <1..1110
                     /gene="hemA"
                     /locus_tag="PLH31_10205"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HQR89610.1"
                     /translation="RPDGGESDVVVWCSNDYLGQGQNPVVLDAMHTAIDDHGSGSGGT
                     RNISGTNHHHVELESELADLHGKEAALLFTSGYVSNEASLSVVQKILPGLIIFSDEQN
                     HASMIAGIRNGGGPRKVFRHNDLAHLEELLAAAPADAPKLVAFESVYSMDGDIADVGA
                     TAALAKKYGALTYLDEVHAVGMYGPRGGGVAERDGVMDQIDIIEGTLAKAFGVMGGYI
                     TGNTEVIDAIRLMASGFIFTTSLPPALTAGALASVKWLKQHPEVREIHQERAQTLKAM
                     FQAAGLPVMENESHIVPVMVGDPTHTKLISDMLLADHGVYVQPINYPTVPRGTERLRF
                     TPTPFHTDDMMRKLVGAMEKLWAHCNVARMGGHAA"
     gene            complement(1126..>1310)
                     /locus_tag="PLH31_10210"
     CDS             complement(1126..>1310)
                     /locus_tag="PLH31_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HQR89611.1"
                     /translation="GKRLKMLKRYLRSHYDMSPEDYRRKWGLQADYPMVAPAYAARRS
                     DFAKQIGLGKGVRRGR"
     assembly_gap    1311..1320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1321..1566)
                     /locus_tag="PLH31_10215"
     CDS             complement(<1321..1566)
                     /locus_tag="PLH31_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HQR89612.1"
                     /translation="MTAQNGDADINGGKSPDLLALSADIVAAYVGQNTVSQSVIPELI
                     RTVHGALSTLNGAEEPRIAEKAKPAVPVSRSIQHDYIV"
     gene            1728..>1847
                     /locus_tag="PLH31_10220"
     CDS             1728..>1847
                     /locus_tag="PLH31_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89613.1"
                     /translation="MTAPASTLTAFFGADLATADRDIFDRIGLELGRQQNQIEL"
     assembly_gap    1848..1857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..>2476
                     /locus_tag="PLH31_10225"
     CDS             <1858..>2476
                     /locus_tag="PLH31_10225"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQR89614.1"
                     /translation="LIASENIVSRAVLEAQGSILTNKYAEGYPGKRYYGGCEYVDEIE
                     IIAIERAKQLFGAGFANVQPHSGSQANQAVFMALLQPGDCFLGMDLAAGGHLTHGSPA
                     NQSGKWFKPVNYTVRQQDQLIDYDNVAEVAEREKPKLIIAGGSAYSREIDFARFREIA
                     DSVGAYLMVDMAHFAGLVAGGAYPSPIPHAHIVTTTTHKTLRGPRG"
BASE COUNT          456 a          789 c          768 g          443 t
ORIGIN      
        1 cgtcctgacg gcggcgaaag cgatgtcgtc gtctggtgca gcaacgacta tcttggccag
       61 ggtcagaacc ccgtcgtgct ggacgccatg cacacggcga tcgatgatca cggctcgggg
      121 tctgggggca cgcgcaacat ctccggcacc aaccaccacc atgttgagct tgaatccgaa
      181 ctggctgacc tgcatggcaa ggaagcggcc ctgctgttca cttcgggcta tgtctccaac
      241 gaggccagcc tgtcggtcgt gcagaagatt ctgccgggcc tgatcatctt ttctgacgaa
      301 cagaaccacg cctcgatgat cgcgggcatc cgtaatggcg gcggcccgcg caaggtgttc
      361 cgccacaacg atctggcgca tctggaagag ctgctggcgg cggctccggc cgacgcgccc
      421 aagctggtgg cgttcgagag cgtctattcg atggacggcg acatcgctga tgtcggcgcg
      481 acggcggccc tggccaagaa atacggcgcc ctgacctatc tggacgaagt acacgccgta
      541 ggcatgtacg gtccgcgcgg cggcggcgtc gccgagcgcg acggcgtcat ggaccagatc
      601 gacatcattg agggcaccct ggccaaggcg ttcggcgtca tgggcgggta catcaccggc
      661 aataccgagg tgattgacgc catccgcctg atggcgtcgg gctttatctt cacgacctcg
      721 ctgccgcctg ccttgactgc tggcgccctg gccagcgtca agtggctcaa gcagcacccc
      781 gaggtgcgcg agattcacca ggaacgcgct cagactctga aggccatgtt ccaggccgca
      841 ggcctcccgg tcatggaaaa cgaaagccac atcgtgccgg tgatggtggg cgacccgacg
      901 cacaccaagc tgatcagcga catgctgctg gccgatcacg gcgtatacgt tcagccgatc
      961 aactatccga ccgtgccgcg cggcaccgaa cggctacgct tcaccccgac gcctttccac
     1021 accgacgata tgatgcgcaa gctcgtcggg gcgatggaaa agctctgggc tcattgcaac
     1081 gtcgcccgta tgggcggcca cgccgcctga ggcggggcga gggggctaac gcccccgtcg
     1141 cacgcccttg cccaggccga tctgcttggc gaagtccgat cgacgcgctg cataggcggg
     1201 cgcaaccatc ggataatcgg cttgcaggcc ccatttgcgc cgatagtcct cggggctcat
     1261 atcgtagtgc gagcgcagat agcgcttcag cattttcagg cgttttccat nnnnnnnnnn
     1321 gacaatgtag tcgtgctgga tcgagcggga aaccggaacg gcgggcttag ccttctcggc
     1381 gatgcgcggt tcttcagcgc cattcagggt cgatagcgct ccgtgcacgg tgcggatcaa
     1441 ttcgggaatg actgactggg agacagtgtt ctgccccaca taggcggcaa caatatcggc
     1501 gctcagcgcc agaaggtcgg gagattttcc gccgttgatg tctgcatccc cgttttgcgc
     1561 cgtcatcggt aatcgccctc tttgttgcta cactgccttg gccgggcgga atcatcgcct
     1621 ggcaggcgac gaacgcgtaa ctttctccga ggttccgaga ttgatcgcgg cgcagagccc
     1681 gttaaagaga gcgccatcga ctcccccgca gcgtctggag accgcccatg accgcaccag
     1741 cttccaccct gaccgccttc ttcggcgctg acctcgccac ggcggaccgt gacatcttcg
     1801 accggatcgg gctggagttg gggcgtcagc aaaaccagat cgagctgnnn nnnnnnnagc
     1861 tgatcgcgtc ggagaacatc gtctcgcggg cggtactgga ggctcagggt tcgatcctga
     1921 ccaacaagta cgccgagggt tatcccggca agcgctacta tggcggctgc gaatatgtcg
     1981 acgagatcga gatcatcgcc attgagcgcg ccaagcagtt gtttggcgcc ggcttcgcca
     2041 acgtccagcc gcattcgggt tcgcaggcca accaggcggt gttcatggcc ctcctgcagc
     2101 cgggagactg cttcctgggc atggacctgg ccgccggcgg ccatttgacg cacggcagcc
     2161 ccgccaacca gtctggcaag tggttcaagc cggtgaatta caccgtgcgc cagcaggacc
     2221 agctgatcga ttatgataac gtcgccgagg tggcggagcg cgagaagccc aagctgatca
     2281 tcgccggcgg cagcgcctac agccgcgaga tcgacttcgc ccgcttccgg gaaattgccg
     2341 acagcgtcgg ggcctatctg atggtcgaca tggcccactt cgcgggtctc gtggccggtg
     2401 gcgcctatcc cagcccgatt ccgcacgccc acatcgtgac gaccaccacg cacaaaaccc
     2461 tgcgcggtcc gcgtgg
//
LOCUS       DAOMEO010000433         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4533_length_2470_cov_2.543271, whole genome shotgun sequence.
ACCESSION   DAOMEO010000433 DAOMEO010000000
VERSION     DAOMEO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_2470_cov_2.543271"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLH31_10230"
     CDS             complement(<1..401)
                     /locus_tag="PLH31_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQR89615.1"
                     /translation="MRWILRGIGGLLALMVLAVVALIIIDRASLQKAPDKATLIAAGE
                     RYDVRIRRDEWGVPHILGKTDADAAYGLGFAQSEDDFATLRDVTLATRGVLARTQGAS
                     AAPTDYVVSLLNVWPTVHANYDKLPADLRKVL"
     gene            573..1328
                     /locus_tag="PLH31_10235"
     CDS             573..1328
                     /locus_tag="PLH31_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR89616.1"
                     /translation="MIRKSMASAVAALALGAVSSAAFAQVPANITAALASPARPAEDT
                     ARDAARHPGEILAFAEVKPGASVVDFIMGGGYFTRILSGAVGSTGTVYAYQPAEFIQF
                     SAKYGENLKTVSAALPNAKPLDGSLLALDLPDSLDLVFTVQNYHDLHLKPFPKATAAG
                     VNAEVFKSLKPGGLFVIIDHAAAAGAGTTVAHDLHRIEIAAVKAEVEAAGFKLEAESP
                     LLRDSSDPHTASVFDPAIRGKTDQFILKFRKPG"
     gene            complement(1341..1499)
                     /locus_tag="PLH31_10240"
     CDS             complement(1341..1499)
                     /locus_tag="PLH31_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89617.1"
                     /translation="MTDAPEETPIQRALRLKKAALDAKPKPPRGGKFQREQAANVQTG
                     KSKPWMTR"
     gene            1573..2103
                     /locus_tag="PLH31_10245"
     CDS             1573..2103
                     /locus_tag="PLH31_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89618.1"
                     /translation="MILRALALTVLLASAATAQVREEPTAVSPVTVMPPTLPPKVVAT
                     YPAQGETIAPGVLIVKVAFDQPMNPRAWNYGVAEGGEQPECIRTPRLLNDQKTFVLLC
                     RVLSNRTYKVALNGERAGGFANLGDNPAETHVLTFQVVRGEPVTSMSRALKAAGLKPE
                     DEPIQEAPPTPPRPAL"
     gene            2156..>2470
                     /locus_tag="PLH31_10250"
     CDS             2156..>2470
                     /locus_tag="PLH31_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89619.1"
                     /translation="MSEETHKTGDWSEADLDLVTGDYFVMLAKQLAGQPVDPASHQRA
                     LRFVTGRPGGAIEFRQRNISAALTLIGIPILKDFAPRWKLHDQLIDAVDRQLSSRPGL
                     IAA"
BASE COUNT          393 a          893 c          804 g          380 t
ORIGIN      
        1 agcaccttgc gcagatcggc cggcagcttg tcgtaattgg cgtgcacggt cggccagaca
       61 ttcagtagcg acacgacata gtcggtgggc gcggccgagg cgccctgggt gcgggccagc
      121 acgccgcgcg tggccagggt gacgtcgcgc agggtggcga agtcgtcctc gctctgggcg
      181 aaacccaggc cataggcggc gtcggcgtcg gtcttgccca gaatgtgcgg cacgccccat
      241 tcgtcgcggc ggatgcggac gtcatagcgt tcgccggccg cgatcagggt cgccttgtcg
      301 ggcgcctttt gcaggctggc gcggtcgatg atgatcaggg cgaccacggc cagcaccatc
      361 agcgccagca gtccgccgat cccgcgcagg atccaccgca tccaagcctc ccccgtcttt
      421 tctcgttgcc gcgatcatgg gggcaagcgg cggggcaggg gaagggcgac gctggggaag
      481 gcgcggcggc gtgttaccga gaattcatga gaacgccgtt cgctggagct ttgaaagctg
      541 gtacccgttg ggaaaaatca gggagacgac gcatgatccg caagtccatg gcctccgcag
      601 tcgcagcgtt ggcgctgggg gccgtcagca gcgccgcctt cgcccaggtt cccgccaaca
      661 tcaccgccgc cctggccagt ccggcccgtc ccgccgagga caccgcccgc gacgcggccc
      721 gccatcccgg cgaaatcctg gccttcgccg aggtgaagcc cggcgccagc gtagtcgatt
      781 tcatcatggg cggcggctat ttcacccgca tcctgtcggg cgcggtaggg tccaccggca
      841 cggtctatgc ctatcagccc gccgagttca tccagttctc ggccaagtat ggcgaaaacc
      901 tcaagaccgt ttcggccgcc ctgcccaatg ccaaaccgct ggacggcagc ctgctggccc
      961 tggacctgcc cgacagtctg gacctggtgt tcaccgtcca gaactatcac gacctgcacc
     1021 tcaagccctt cccgaaggcc acggccgccg gggtcaatgc cgaggtgttc aagtcgctga
     1081 agccgggcgg gctgttcgtg atcatcgacc acgccgccgc cgccggcgcc gggaccacgg
     1141 tggcccacga cctgcaccgc atcgagatcg ccgccgtcaa ggccgaggtc gaggccgccg
     1201 gcttcaagct ggaggccgaa agcccgctgc tgcgcgactc cagcgatccg cacaccgcca
     1261 gcgtcttcga cccggccatt cgcggcaaga ccgatcagtt catcctgaag ttccgcaagc
     1321 ccggctaggg cgcgccgcgt ctagcgggtc atccagggct tggacttgcc ggtctggaca
     1381 ttggcggcct gctcgcgctg gaacttgccg ccgcgcggcg gtttcggctt ggcgtcgagg
     1441 gcggccttct tcaggcgcag ggcgcgctgg atcggagttt cttcgggagc gtcggtcatg
     1501 acggcgccta tagcacaggc gcggcgcgag gggcgccttg agaacgccca gcttgccggt
     1561 caagctgcgc ccatgatcct tcgcgccctc gccctgaccg tccttctcgc cagcgccgcg
     1621 accgcccagg tgcgcgagga gccgaccgcc gtctcgcccg tcacggtgat gccgccgacc
     1681 ctgccgccca aggtggtggc cacctatcct gcgcagggcg agaccatcgc ccccggcgtg
     1741 ctgatcgtga aggtcgcctt cgaccagccg atgaacccca gggcctggaa ctacggcgtc
     1801 gccgaaggcg gcgagcagcc cgaatgcatc aggacccccc gcctgctcaa cgaccagaag
     1861 accttcgtac tgctgtgccg ggtgctgtcg aaccgcacct acaaggtcgc cctcaacggc
     1921 gagcgggccg ggggcttcgc caacctgggc gacaaccccg ccgaaaccca tgtcctgacc
     1981 ttccaggtgg tgcgcggcga gcccgtgacc tcgatgtctc gcgccctgaa ggcggcgggt
     2041 ctgaaacccg aagatgagcc cattcaggaa gcgcccccga cgccgccccg ccccgccctc
     2101 tagctcgggc caagctttgg tggcgcgacg gccccgcctg aggcatcgtc gcgccgtgag
     2161 cgaggaaacc cacaagaccg gagactggag cgaggccgat ctggacctcg tgaccggcga
     2221 ctatttcgtc atgctggcca agcagttggc cggccagccg gtcgatccgg ccagccacca
     2281 gcgcgccctg cgattcgtca ccggccgtcc cggcggggcc atcgagtttc gccagcgcaa
     2341 catctcggcc gccctgaccc tgatcggcat cccgatcctg aaggacttcg ccccgcgctg
     2401 gaaactgcat gaccagctga tcgacgccgt cgaccgccag ctctccagcc ggcccggcct
     2461 gatcgccgcc
//
LOCUS       DAOMEO010000434         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4536_length_2468_cov_2.889764, whole genome shotgun sequence.
ACCESSION   DAOMEO010000434 DAOMEO010000000
VERSION     DAOMEO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_2468_cov_2.889764"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLH31_10255"
     CDS             <1..1057
                     /locus_tag="PLH31_10255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQR89620.1"
                     /translation="AFRAAYSGEHTPAGMSALLADTVRSQTTPQGTFSYTNAGYNLAT
                     VLIEDRFGRDWRTLVNREVLAPAGMTHTTARIDQARQKMLVAAGHTPGPDGPTAPSSL
                     QKTNATMHSAGGLVSTARDMARWLEIQLNDGVVDGHRAFPPGLVASTHRPLATQQARF
                     GPYTREGYGLGWQTGHYGTEALIHHFGNFAGSRAHVSFMPARGLGVAVMANEDLLAGE
                     LADLVANYVYDRFAGRPDLEAHYDAELAALTARRDKRVKGLAASRAERASRPWSLRLP
                     TKSYVGDYRNPAMGSVQVVEEDGRMVIRAGVMASPAEAVPNPESVRVELIPLRGQTVR
                     FETPGRLVFDGQTYVRR"
     gene            complement(1314..1931)
                     /gene="pth"
                     /locus_tag="PLH31_10260"
     CDS             complement(1314..1931)
                     /gene="pth"
                     /locus_tag="PLH31_10260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQR89621.1"
                     /translation="MLILAGLGNPEAKYEKNRHNVGFMAVDALARRWGTGPWRARFQG
                     LACEGQVGTPNGPVKLLLLKPKTYYNESGRAVGEAMKFFKLAPTDVIVFHDEIDMAPG
                     RFRMKAGGGAAGNNGIRSVTSQVGDTFRRGRIGVGHPGDKAMVMHYVLGDFHKVEHQW
                     LDPLLDAICDALPFAAAGEDERYQAEVLRLAPAPKADPRKPAPES"
     assembly_gap    2182..2191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2192..>2468)
                     /locus_tag="PLH31_10265"
     CDS             complement(<2192..>2468)
                     /locus_tag="PLH31_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQR89622.1"
                     /translation="IAKAVQFHAVTDEPVHFDLYRVDEHQLIKIEVPVHFKNHETSVG
                     LKKGGTLEIIRHTVELACPADKIPEELVIDLAGHDIGDVIRISEVKLP"
BASE COUNT          402 a          823 c          810 g          423 t
ORIGIN      
        1 cgccttccgc gcggcctatt cgggcgagca tacgccagca ggcatgagcg ccctgctggc
       61 ggacacggtc cgcagccaga ccacgcccca gggcacgttc agctacacca atgcgggcta
      121 taatctggct acggtcctga tcgaagaccg ctttggacga gactggcgca cgcttgtgaa
      181 ccgggaggtt ctggcgccgg ccggcatgac ccacaccacg gcccggatcg accaggcgcg
      241 tcagaagatg ctggtcgcgg cgggccacac gccaggtccc gacgggccca cggcgcccag
      301 ttcgctgcag aagaccaacg ccaccatgca ttcggccggg gggctggtct cgacggcccg
      361 ggacatggcc cgatggctcg agatccagct gaacgacggc gtcgtggatg gacaccgcgc
      421 gtttccgccg gggctcgtcg cctccaccca tcgaccactg gcgacccagc aggccaggtt
      481 tggtccctac acgcgcgaag gctacggtct gggctggcag acgggccact acgggaccga
      541 ggcgctgatc caccatttcg gcaatttcgc cgggtctcgc gcccacgtct cgttcatgcc
      601 ggcgcgcggt ctgggcgtgg cggtgatggc caacgaggat ctgctggccg gcgaactggc
      661 cgatctggtg gcgaactatg tctatgaccg tttcgccggc cgccctgacc tcgaagccca
      721 ttatgacgcc gagctggcgg cgctgacggc gcgtcgtgac aagcgggtca agggtctggc
      781 cgccagccgg gccgagcgcg cctcgcgccc ctggtccctg cggctgccga ccaaatccta
      841 cgtcggagac tatcgcaacc cggccatggg cagcgtccag gtggtcgagg aagacggtcg
      901 catggtgatc cgcgcggggg tgatggcctc accggccgag gccgtcccca atcccgaaag
      961 cgttcgggtc gagcttatcc cccttcgcgg acagacggtt cgctttgaaa cacccggcag
     1021 gctcgtgttc gacggccaga cctacgtccg tcgctagcgg cgccgtcaac gggcaaagcg
     1081 tcggctttcg cctaagcggc cggagatctg cggctaccgc acggcgacat gactacgatc
     1141 gaatccaaca tcggcaacca ctctcgaaca gacgatcacc atgtccgctc ccggcgccgg
     1201 cgaccgacct ttgccagcgt cggtgcattc cctcatttca gggcgggaat ggcggtgggg
     1261 aaagcacaaa atggccggct gaatttcgat aaggcctggt cttccgaccc gatctagctt
     1321 tcgggggccg gcttccgggg gtcggccttc ggcgcgggcg ccaggcgcag cacctcggcc
     1381 tgatagcgtt cgtcctcgcc cgccgccgcg aagggcaggg cgtcgcagat cgcgtccagc
     1441 agcgggtcaa gccactggtg ctcgaccttg tggaagtcgc ccagcacgta gtgcatgacc
     1501 atggccttgt cgcccgggtg acccacgccg atgcggccgc gccggaacgt gtcgcccacc
     1561 tggctggtga ccgagcggat gccgttattg cccgccgccc cgccgccggc cttcatccgg
     1621 aaacgccccg gcgccatgtc gatctcgtcg tggaacacga tgacgtcggt gggagccagc
     1681 ttgaagaact tcatcgcctc gcccacggcg cggccgctct cgttgtaata ggtcttgggt
     1741 ttcagcagca gcagcttgac cgggccattc ggcgttccga cctgaccttc gcaggccagc
     1801 ccctggaagc gcgcgcgcca cggccccgtg ccccagcgcc gggccagggc gtcgacggcc
     1861 atgaagccga cattgtggcg gttcttctcg tacttggcct cgggattgcc gagaccggcc
     1921 agaatcagca tgactgaggc tccgtgcgtc cttcgaggcc ttgctgcgca aggcgcctca
     1981 ggatgaggaa ttgtttagtc gcgcctcgac cccgctcctc atcctgaggc gccgcgttag
     2041 cgggcctaga agggcgcagg atcttggcca aaacgaaaaa cgggcggacc cgaaagtccg
     2101 cccgtctcga tatcagatcg gagacccgac caaaggtttt tagccctcgg cggtggtgtc
     2161 gccttcgccc gattgcgacg cnnnnnnnnn ncggaagctt cacttccgag atgcggatga
     2221 cgtcgccgat gtcgtggccg gccaggtcga tgaccagttc ctcggggatc ttgtcggccg
     2281 ggcaggccag ctcgacggtg tgacggatga tttccagcgt gccgcccttc ttcaggccga
     2341 ccgaggtttc gtggttcttg aagtgaaccg gaacttcgat cttgatcagc tggtgctcgt
     2401 cgacgcggta caggtcgaag tggaccggct cgtcggtgac ggcgtggaat tgcacggcct
     2461 tggcgatg
//
LOCUS       DAOMEO010000435         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4545_length_2466_cov_2.587308, whole genome shotgun sequence.
ACCESSION   DAOMEO010000435 DAOMEO010000000
VERSION     DAOMEO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4545_length_2466_cov_2.587308"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLH31_10270"
     CDS             <1..666
                     /locus_tag="PLH31_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR89623.1"
                     /translation="VASLEGETRLRVTLDGLAIGDLLLADALDPSAADGVARLKRRGL
                     QVVMATGDAEGPALAVAQRIGLDAAAVHWGCTPADKADRVRNTSGPVLFVGDGVNDAP
                     ALTAAACGVSVARAHPAATATAAIVILRGGLEQVDVALSLAEETRRTIRQNVVLALAY
                     NIVVLPLAALGLLSPLGAAVAMTASSLLVMLNALRGPQTRLSREASSSAGDDGRRQVR
                     PSP"
     gene            complement(887..1300)
                     /locus_tag="PLH31_10275"
     CDS             complement(887..1300)
                     /locus_tag="PLH31_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR89624.1"
                     /translation="MIISSMPGDVVQAFAREIAFRDVRRLESLVADDIDARFDVAGIL
                     VGKAALLGFWRRLFQAYSLVELHILKCVVEEDLVIADSAYFLAARRGGLVDVRAISIF
                     EVDQGVITHWRDHADLAEVPKAETDLWRRLGGARW"
     gene            complement(1366..1605)
                     /locus_tag="PLH31_10280"
     CDS             complement(1366..1605)
                     /locus_tag="PLH31_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89625.1"
                     /translation="MTPHRQTDVGTQNLALSPRELECLTWVCRGKSSGDIGSILGLSP
                     RTVDSYLEKVAAKLRVRTRIEAVAVAVRAGLIEPD"
     assembly_gap    2124..2133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2170..>2466
                     /locus_tag="PLH31_10285"
     CDS             2170..>2466
                     /locus_tag="PLH31_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQR89626.1"
                     /translation="MKRPTGWLGKRLRGRVGVDEADDVVQETYLRLTPYLLEDIRHPK
                     ALLLRVALNLVRDERRRLVRRERALQGTGSTLDTAADAPVDRLYLKQVLLTMPPL"
BASE COUNT          395 a          821 c          795 g          445 t
ORIGIN      
        1 gtcgcaagcc tggagggaga aacgcgtctt cgggtcaccc tggatggtct cgcgatcggc
       61 gatcttctgc tcgccgatgc gctggacccc agcgccgctg acggcgtggc gcggctcaag
      121 cggaggggat tacaggtcgt catggccacc ggcgacgccg aggggccggc tctggcggtc
      181 gcgcagcgga tcggcctgga cgccgctgcg gtccattggg gttgcacccc cgccgacaag
      241 gccgacaggg tcaggaacac gagcggacct gtgctcttcg tcggcgacgg cgtgaacgac
      301 gcccctgcct tgaccgcagc cgcctgcggg gtttcggtgg cccgggcgca tccggccgcg
      361 acggccacgg cggcaatcgt catcctgagg ggcggtcttg agcaagtcga tgtggcgctg
      421 agcctggctg aagagacgcg acgcaccatc cgccagaatg tggtcctcgc cctcgcctac
      481 aacatcgtcg ttctgcccct ggcggccttg ggcctgttgt cgcccttggg ggccgcagtc
      541 gccatgaccg cgagttcgct gctggtgatg ctgaatgcgc tgcgcgggcc acagacgcgg
      601 ctgtctcgcg aggcgtcgtc gtcggcaggc gacgatggcc gccgccaggt gcgtccttcg
      661 ccttgagcac gcgtcggatc ggcagcgccc gacgatcctg gccttgggcg ggcttggttt
      721 tccctttcag gggcgtagga tgtcggacgg cgggcctcgt ccggatagcc ttcgatctgg
      781 agccagggga cgcaatgcgt cggcagcgat cgcgggtgaa gacgtcgagc ttcacacaga
      841 agttcccggc ccgccccctc cgcctacgca gccggtctgg gtgcggctac catcgggctc
      901 cgcccaagcg gcgccagagg tccgtttccg ccttgggcac ctccgccaga tcggcatgat
      961 cccgccagtg ggtgatgacg ccctgatcga cttcgaagat gctgatcgcg cgcacgtcca
     1021 ccagaccgcc ccggcgggcg gccaggaaat aggcgctgtc ggcgatgacc agatcctcct
     1081 caacgacgca tttgaggata tggagttcga cgagcgaata ggcctgaaac agccgccgcc
     1141 agaacccgag cagcgcagct ttaccgacca gaatgcccgc gacgtcgaac cgggcgtcga
     1201 tatcgtctgc gaccagggac tcaagccgtc ggacgtcgcg aaacgcgatc tccctagcga
     1261 aagcttgaac gacgtctccg ggcatggacg aaatgatcac ggttcagatg atcgatcatg
     1321 acccgattct cgatgaaaaa tcgcgcgtcg cgtgtggacc cctcatcagt ccggctcaat
     1381 caagccggcc cggaccgcaa ctgcgacagc ctcgattctc gtccggaccc ggagtttcgc
     1441 agcgaccttt tccaaatagc tatcgaccgt gcgcggggac agtcccagga tgctgccgat
     1501 gtcgcccgaa ctcttgccgc ggcaaaccca ggtgaggcat tcgagttctc gcgggctcaa
     1561 tgcgaggttc tgtgttccga cgtctgtctg acggtgtggt gtcatgggat cactcgcttt
     1621 tagaggcgca cgatgacgcg gtccgtcagc ctgaacaacg ccgtcgattt gcggagactc
     1681 cgcaacaaac ttgcggaaac cccggagcgg ttttcagcag cgccgcgcgt ctgtcgcgct
     1741 ttacccatca cttgccctgc aggctcccga gccctaaatc ctgagacgtc gggcgccgcc
     1801 agataggggc ggaacttgag ccagggactg cctttggcgg tggcggcgtt tcatcctgaa
     1861 gtagcgggcc tcttcgcaga ggattcctcg cccgacccct ccgccttgca ccgcgactcg
     1921 gattgattca ttgtcggttg tcggcgtccg cgtcgatagg ggccgggaat cccctgtgcg
     1981 aagctcaacc ttaacggccg cgccttgacg cgcggcgtcc gtccgccatc gcctgcggcg
     2041 cacctttgcg tgcgcccgga cgccacgcgg tcgcgctctc ggattcccgc cgtctgacag
     2101 ccggctggag gtcgcgttga gcgnnnnnnn nnncactggg cgcggctatt tctgaattcc
     2161 ccatcaacct tgaaacggcc cacgggatgg ctgggcaagc ggcttcgtgg acgcgtcggc
     2221 gtcgatgagg ccgacgacgt ggtgcaggaa acctatcttc gcctgacgcc gtaccttctc
     2281 gaagacatcc gccatccgaa agccttgctt cttcgggtcg cgctcaacct cgtccgcgac
     2341 gaacggcgcc gtcttgtgcg ccgcgagcgc gccttgcagg gtacgggttc gaccttggac
     2401 acggcggccg acgcgcctgt cgatcgcctc tacctcaagc aggtcctcct gacgatgccg
     2461 cccctc
//
LOCUS       DAOMEO010000436         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4550_length_2464_cov_2.462017, whole genome shotgun sequence.
ACCESSION   DAOMEO010000436 DAOMEO010000000
VERSION     DAOMEO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_2464_cov_2.462017"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLH31_10290"
     CDS             complement(<1..147)
                     /locus_tag="PLH31_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HQR89627.1"
                     /translation="MPQSLTLAEARAFLRASDASEDATITLLIEAAEARVAAATGLTL
                     TPASP"
     gene            complement(296..1516)
                     /locus_tag="PLH31_10295"
     CDS             complement(296..1516)
                     /locus_tag="PLH31_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HQR89628.1"
                     /translation="MKETKHAPASPGAMMHEVLAAFEAFKAANDQRLSAIETKRADVL
                     LEEKVSRIDEAVSRAQDRLDRVLADSRRPSLAGDAPLARVDERKAAFDRYIKTGETPG
                     LLLEAKGLSEGVATAGGYVAPPELERLILRRLAATSPMREICQVRTIGAGVFRKPVSP
                     TGLAAAWVAETAARPETTAPTLDIIDFPAGELYASPAATQALLDDAYVSIDEWLAEEV
                     QDAFAAQETTAFISGDGVNKPKGLLAYTAAPDASYTWGQMGYLATGVAGGFAAANPTD
                     KLIDLIYATKTQYRQNGRFVMNRRTVSVVRKFKDSQGNYIWNAALQPGQSASLLGYPV
                     TEIEAMPDVAANSLAVAFGDFEKGYLITDRAGVRVLRDPYSAKPHVLFYTTKRVGGGV
                     QNFDAVKLLKFAVS"
     gene            1708..2073
                     /locus_tag="PLH31_10300"
     CDS             1708..2073
                     /locus_tag="PLH31_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="HQR89629.1"
                     /translation="MTDHATPNLPSRDFETTARFYAALGFEQGWRDNGWMILKRGSLM
                     LEFFPYPDLDPASSSFGSCLRLDDVDGFYAVCQAAGLPETHLGWPRLHRPRLEASGLR
                     IGALIDPDGSLLRLIQNPD"
     gene            2131..>2464
                     /locus_tag="PLH31_10305"
     CDS             2131..>2464
                     /locus_tag="PLH31_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89630.1"
                     /translation="MTSKLTALERAVAEAIAREYPDATDALLRQLQNAVVTSRDFTGV
                     GFFTEFDVPRDGPPAKDLVGPVGHICALVGSERYPLEFMLYVRGGYAEMIEAYSFEDG
                     YGDLDLLTA"
BASE COUNT          365 a          811 c          863 g          425 t
ORIGIN      
        1 ggggctggcg ggggtcaggg tcaggccggt ggcggcggcg acgcgggcct cggcggcctc
       61 gatcagcaag gtgatcgtgg cgtcttccga ggcatcggac gcacgcagaa acgcccgggc
      121 ttcggccagg gtgagggatt ggggcatgga ggattttcct ggaaagggcg gcgctgcccc
      181 ctcagtcgct ccgcgacagc tcccccagcg ggggagcagc tgcgcggcgt agatcctccc
      241 cctctggggg aggtggccca gagggccgga gggggcagcg ccaaggctgg ccgaattagc
      301 tgaccgcgaa cttcagcagc ttcacggcat cgaagttctg caccccgccg ccgacgcgct
      361 tggtggtgta gaacagcacg tgcggcttgg cggagtacgg gtcgcgcagc acccggacgc
      421 cggcgcggtc ggtgatcaga tagcccttct cgaagtcgcc gaacgccacc gccaggctgt
      481 tcgccgcaac atcgggcatg gcctcgatct cggtcaccgg atagcccagc aagctggccg
      541 actggcccgg ctgcagggcc gcgttccaga tgtagttgcc ctggctgtcc ttgaacttgc
      601 gcaccacgct gaccgtgcgg cggttcatca cgaagcggcc gttctggcga tactgggtct
      661 tggtggcgta gatcaggtcg atcagcttgt cggtgggatt ggccgccgcg aaaccgccgg
      721 cgacgccggt ggcgaggtag cccatctgac cccaggtgta gctggcgtcc ggcgcggcgg
      781 tgtaggccag caggcccttg ggcttgttga cgccgtcgcc gctgatgaag gccgtggtct
      841 cctgcgccgc gaaggcgtcc tgcacctcct cggccagcca ctcgtcgatg ctgacatagg
      901 cgtcgtcgag cagggcctgg gtcgcggcgg ggctggcgta gagctcgccg gccgggaaat
      961 cgatgatgtc cagggtgggc gccgtggtct ccggacgcgc cgcggtctcg gccacccagg
     1021 cggcggcaag gccggtgggc gacaccggct tgcggaacac gccggcgccg atggtgcgga
     1081 cctgacagat ctcgcgcatc gggctggtcg ccgccagacg acgcaggatc agccgctcca
     1141 gctccggcgg cgccacatag ccgccggccg tggccacgcc ttccgacagg cccttggctt
     1201 ccagcaacaa gccgggcgtt tcgccggtct tgatatagcg gtcgaaggcg gccttgcgct
     1261 cgtccacccg cgccagcggt gcatcaccgg ccagggaagg cctgcggctg tcggcgagca
     1321 cccggtccag acggtcctgg gcgcgggaga cggcctcgtc gatgcggctg accttctcct
     1381 ccagcagcac gtcggcccgc ttggtctcga tggcgctcaa tcgctggtcg ttggcggcct
     1441 tgaaggcctc gaatgcggcc agcacctcat gcatcatcgc gcccggcgag gccggggcgt
     1501 gtttggtctc tttcatggag ttctccgaaa aagcccttcc cccttgatgg gggaagggtt
     1561 tgggatgggg gtgactacgg cggtgaccgg tcaccccgac ggtcggatgg aggcgcggcg
     1621 ccggacggct cgtcctgacg cgctgtcacc cccatccccg cccttccccc atcgaagggg
     1681 gaagggagtt ctgttagcgt tcgccgcatg accgatcacg ccacgcccaa cctgccgtcc
     1741 cgcgacttcg aaaccacggc ccgcttctat gcggcgctgg ggttcgagca gggctggcgc
     1801 gataacggct ggatgatcct gaagcgcggg tcgctgatgc tggaattctt cccgtatccg
     1861 gacctggatc cggcgagcag ctcgttcggc tcgtgcctgc ggctcgacga cgtcgacggg
     1921 ttctacgccg tgtgccaggc cgccggcctg cccgagacac acctgggctg gccccgcctg
     1981 catcggccca ggctggaagc cagcggcctg cgcatcggag ccctgatcga ccccgacggc
     2041 tcgctgctaa gactgatcca gaacccggac tagccgcgcg tcgggcttct tcaatcttgg
     2101 ctgcagcgat gatcccccgt aggttgcgcc atgacgtcga aactcaccgc ccttgaaagg
     2161 gctgtcgctg aagctattgc ccgggaatat cccgacgcca cggatgcatt gctccggcag
     2221 cttcagaacg ctgtcgtcac cagccgagac ttcactggcg tcggcttctt caccgagttc
     2281 gacgtgccta gggatggccc gccagccaag gacttggtcg gcccggtggg gcatatctgc
     2341 gcattggtcg ggtctgagcg gtacccgctg gaattcatgc tctacgtccg cggcgggtac
     2401 gctgagatga tcgaggccta cagcttcgag gacggctacg gcgatctgga tttgttgacg
     2461 gcgg
//
LOCUS       DAOMEO010000437         2463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4551_length_2463_cov_2.955565, whole genome shotgun sequence.
ACCESSION   DAOMEO010000437 DAOMEO010000000
VERSION     DAOMEO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4551_length_2463_cov_2.955565"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLH31_10310"
     CDS             <1..211
                     /locus_tag="PLH31_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQR89631.1"
                     /translation="LTAYEREGVAAKAQTPSQSHFFPALEDMVTVGYFTSEPGATVAL
                     QYDPVPGAYRGCVPLAEIGRAWATR"
     gene            complement(208..1395)
                     /locus_tag="PLH31_10315"
     CDS             complement(208..1395)
                     /locus_tag="PLH31_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HQR89632.1"
                     /translation="MILPPTRAAALQTLAEFVPRADRSYASGRNIDPGPDQPAAVSRL
                     SAYVRHRLITEPEIIAAVLSQHDMASAQMFIQEVLWRSYWKGWLEMRPSIWTRFTTER
                     DRQSEIFANTPALAEALAGRTGIEGFDDWVLELRQTGYLHNHARMWFASIWIFTLQLP
                     WTLGADFFLRHLIDADAASNTMSWRWVAGLQTPGKTYLASTDNIARYTNHRFAPKGLA
                     TEARVIAEPPIPPACALPDSHRAKPSQPAILLVTHEDMHPESLFAPITPFEATVVAID
                     ERLLWGKAAHDFVKAAAVDTAARAEKAFGRPATLCDSLDAESLLSAAKAAGAQQIITP
                     YAPVGPVADALKRLAPALANEGVTLVQARRRWDDQLWPHAIKGFFPFKARAMSILADG
                     DLT"
     gene            complement(1506..>2463)
                     /locus_tag="PLH31_10320"
     CDS             complement(1506..>2463)
                     /locus_tag="PLH31_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR89633.1"
                     /translation="TADVDVAVEMDPAVLTRDWVRAAARRGMNRASLGVQNLTPEVQA
                     AVNRRDTFEHIADCVAWLREARIGSINIDLMYGLPHQTTANTLSTLDQLLPLRPERLA
                     LFGYAHVPWMKSHQQLIDEAALPDAQARLDQSETAAERLAAEGYVRIGLDHFALPEDP
                     MAKAQAAGTLRRNFQGYTTDPAVTLLGMGASAIGCLPQGFVQNAAHELTWRAAVKEGR
                     LPIARGVAVDAEDRFRAEIIERLMCDFHVDLAAVCARHGRALSDLAEDVSRLAPFIAD
                     GLAQVRGARVEVLPAGQLLARSIAAVFDAYFSAESQRHARAI"
BASE COUNT          411 a          784 c          868 g          400 t
ORIGIN      
        1 gctgacggcc tatgagcgcg aaggcgttgc ggccaaggcg cagacaccca gccagtccca
       61 ctttttcccg gccctcgaag acatggtgac ggtgggctat ttcacctccg aacccggcgc
      121 cacggtggcg ctgcagtatg atcccgtgcc cggcgcctat cgtggctgcg tgccgctggc
      181 ggagatcggg cgcgcctggg cgacccgcta ggtcagatcg ccgtccgcga ggatggacat
      241 ggcgcgcgcc ttgaagggga agaaaccctt gatcgcgtga ggccagagct ggtcgtccca
      301 gcgacgtcgc gcctggacca gcgtcacacc ctcattggcc agggccggag ccaggcgttt
      361 gagggcgtcc gcgacgggac cgaccggcgc atagggcgtg ataatctgtt gggcgcctgc
      421 cgccttggca gccgacagca ggctctcggc atccaggctg tcgcagagtg ttgcggggcg
      481 cccgaaagcc ttctccgcac gggctgccgt atccacagcc gcagctttga cgaaatcatg
      541 ggccgccttg ccccagagca ggcgctcatc gatcgcgacg acggtcgcct cgaagggcgt
      601 tattggtgcg aaaagcgact ccgggtgcat gtcctcgtgg gtcacaagca ggatcgcggg
      661 ctgggaaggc ttggcgcggt gcgagtctgg cagcgcgcaa gccggcggta tcggcggctc
      721 tgcgatcacc ctggcctccg tcgccaggcc cttgggcgca aagcgatggt tggtgtagcg
      781 cgcgatgttg tcggttgagg cgagataggt cttgcccggg gtctgcagtc ccgcgaccca
      841 gcgccaggac atggtgttgc tggccgcgtc agcgtcgatc agatggcgaa ggaaaaagtc
      901 cgcgcccaac gtccagggca gctgcagcgt gaaaatccag atggaggcga accacatgcg
      961 ggcatggttg tggaggtatc cggtctggcg cagttccagc acccagtcat cgaacccctc
     1021 gatgcccgtc cgccccgcca gggcctcggc cagggcaggg gtgtttgcga agatctcgct
     1081 ctggcgatcc cgctccgtcg tgaaccgggt ccagatggac gggcgcattt ccagccagcc
     1141 cttccaatag gagcgccata gcacctcctg gatgaacatc tgggctgacg ccatgtcgtg
     1201 ctgcgacagc acggcggcaa tgatctctgg ctcggtgatc aggcgatggc gcacataggc
     1261 cgaaagtctc gagacggcgg ccggctggtc ggggcccgga tcaatattgc gcccggacgc
     1321 ataggatcga tccgccctgg ggacaaattc ggcgagcgtc tgcagcgcag cggcgcgggt
     1381 gggcggcaag atcatgtcga ccgtgagaaa gggctcgacg cgtccagagc gacgcgacgc
     1441 tgggctagct cttaagctca cggatggccc aggctccacc tcttttaggg ttttgcctgc
     1501 gaacgtcaga tcgccctggc atggcgctgg ctttcagcgc tgaaataggc atcgaacacg
     1561 gcggcgatgg accgcgccag cagttggccg gccggcagga cttcgacccg cgctccgcga
     1621 acttgcgcca ggccgtcggc gatgaagggc gccaggcgag agacgtcctc cgccaggtcg
     1681 ctaagggccc gtccatgccg ggcgcagacc gccgccaggt cgacgtggaa gtcgcacatg
     1741 agacgctcga tgatctccgc ccgaaagcgg tcctccgcat cgacggcgac gccccgggcg
     1801 atgggcaggc gaccttcctt gacggcggcg cgccaggtca gctcatgggc ggcgttttgc
     1861 acgaagccct gaggcaggca gccgatcgcc gaagcgccca tgcccagcag ggtgacagcg
     1921 ggatctgtgg tgtagccctg gaaattgcgg cgcagggttc cggcggcctg ggctttggcc
     1981 atcggatcct cgggcagggc gaaatggtcg aggccgatcc ggacgtagcc ctctgccgcc
     2041 agacgttcgg cggcggtctc gctctgatcc agccgcgcct gggcgtcggg cagggcggcc
     2101 tcgtcgatca gctgctgatg acttttcatc cacggcacat gggcgtagcc gaacagggcc
     2161 aggcgctcgg gacgaagcgg caggagctga tcgagcgtcg acagggtgtt ggcggtggtc
     2221 tgatgcggca gaccatacat caggtcgata ttgatcgaac cgatccgggc ctcgcgcagc
     2281 caggcgacac agtcggcgat gtgctcgaag gtgtcgcggc gattgacggc cgcctggacc
     2341 tcgggggtca ggttctggac gcccaggctg gcgcggttca tcccccgccg ggccgcggcg
     2401 cgcacccagt cgcgcgtcag cacggcggga tccatctcca ccgccacatc gacgtcggcg
     2461 gtc
//
LOCUS       DAOMEO010000438         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4565_length_2457_cov_2.509992, whole genome shotgun sequence.
ACCESSION   DAOMEO010000438 DAOMEO010000000
VERSION     DAOMEO010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_2457_cov_2.509992"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PLH31_10325"
     CDS             complement(<1..1669)
                     /locus_tag="PLH31_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQR89634.1"
                     /translation="MGRAGKIAAHTLLAFLAFLAGRVLAGVTTIDMHVLPQAALFAAS
                     ALLVELVLRVERVPWRFVAASDILRLARSAFATCLVFAALLWLTTQRFEGLRALIGAF
                     VIQVFLLASLRVVRRGLHEHVLFESILGLRPAAAHPAQPRLLIIGAAAEAEEFLRAPT
                     GLGERYTPVGVVSPLDKETGDELRGVCVLGSIAAFDDVLAQLREGGLAPAAILFLADS
                     PMSAFGAERLGRLKSEGMRLLRRHGVVEMGHTATALREISLEELLSRPPVHLDPEPVR
                     TLVAGRRVLVTGAGGSIGSELCRQIAASGCARLTMLDVSEANLYHIDREIGEAWPTLS
                     RREILCDVRDAARLDRVFAAERPEIVFHAAALKHVTMVENHPCEGARTNVLGTRNVAM
                     AAKAFGAAHMALISTDKAVAPTSVMGATKRVAEALARQYGSSHETRVSIVRFGNVLGS
                     AGSVVPLFQHQIARGGPITLTDAEVERFFMTIPEAVQLVLRAVALSANSPEPPAGVLT
                     LEMGEPVKIIDLARRMIELQGLVPDRDIEIRITGLRPGEKLTELLVDV"
     gene            complement(1768..2373)
                     /locus_tag="PLH31_10330"
                     /pseudo
     CDS             complement(1768..2373)
                     /locus_tag="PLH31_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
BASE COUNT          408 a          797 c          915 g          337 t
ORIGIN      
        1 tgacgtcgac cagcaactcg gtgagctttt cgccggggcg caggccggtg atgcggatct
       61 cgatgtcgcg gtcggggacc aggccctgca gctcgatcat tcgccgggcc aggtcgatga
      121 tcttgaccgg ctcgcccatt tccagggtca gaacgccggc cggcggctcg ggcgagttgg
      181 ccgacagggc cacggcgcgc aaaacgagct gcaccgcctc cgggatggtc atgaagaacc
      241 gttcgacctc ggcgtcggtc agggtgatcg ggccgccgcg ggcgatctgg tgctggaaca
      301 gcggcaccac ggaacctgcc gagcccagca cattgccaaa gcgcacgatg ctgacgcggg
      361 tctcatggct ggagccatac tggcgggcca gggcctcggc gacgcgcttg gtggcgccca
      421 tcacgctggt gggggccacg gccttgtcgg tggagatcag ggccatgtgc gccgcgccga
      481 aggccttggc ggccatggcc acgttgcggg tgcccagcac attggtgcgg gctccttcgc
      541 agggatggtt ctcgaccatg gtgacgtgct tgagcgcggc ggcatggaag acgatctcgg
      601 gccgctcggc ggcgaagacg cggtccagcc gggcggcgtc gcggacgtcg cacaggattt
      661 cgcgccgcga cagggtgggc caggcctcgc cgatctcgcg gtcgatgtga tagagattgg
      721 cctccgagac gtccagcatc gtcagtcggg cgcagccgct ggcggcgatc tggcggcaga
      781 gttcggagcc gatgctgccg ccggctccgg tgaccagcac gcgccggcca gccaccaggg
      841 tgcggaccgg ttcgggatcc agatgcacgg gcgggcggct cagcagctct tccaggctga
      901 tttcgcgcag ggcggtggcg gtgtggccca tctcgaccac gccatggcgg cgcagcaggc
      961 gcatgccttc gcttttcaac cggcccagac gctcggcgcc aaaggcgctc atcgggctgt
     1021 cggccaggaa caggatggcg gccggcgcca ggccgccttc gcgcagttga gccaggacgt
     1081 cgtcgaacgc cgcgatggag cccagcacgc agacgccgcg cagttcgtcg ccggtttcct
     1141 tgtccagggg cgagaccaca ccgaccggcg tgtagcgctc gcctaggccg gtcggggcgc
     1201 gcaggaactc ttcggcctcg gccgcagcgc cgatgatcag cagacggggc tgggcgggat
     1261 gggccgcggc ggggcgcagg cccaggatcg attcgaacag gacatgctca tgcaggcccc
     1321 ggcggacgac gcgcaggctg gccagcagaa acacctggat cacgaaggcg ccgatcaggg
     1381 cgcgcaggcc ttcgaaacgc tgggtggtca gccagagaag ggcggcgaac accaggcagg
     1441 tggcgaaggc cgaacgcgcg aggcgaagga tgtcgctggc ggccacgaag cgccacggca
     1501 cgcgctcgac gcgaagcaca agttcgacca gcagggccga ggcggcgaag agagcggcct
     1561 gcggcaggac gtgcatgtcg atcgtggtga cgccggcgag gaccctgcca gccaggaagg
     1621 ccaggaaggc tagcagcgtg tgcgctgcga tctttccggc acggcccata gacgaaacct
     1681 ctacttcaaa cacccggccg agacccggag tcgtccgggc ttagcacgcc tgctcgccgc
     1741 cgtcctccct gtcaccccac gctttcgtca ggtgcgtgcc aggggcagaa catcgacgat
     1801 gtcgcctgcg gacgcagcga ccgcgttcac ggagcgccgg acgagagcat cggcccggga
     1861 gaagacgccg accagcgagg aatcctgatc ggcgaatgcg gcgacgatcg ctcgcccctg
     1921 gggatcgatc gacagggcgg cgcgcatcca gtgttcgcgg gggccattgg ggggcaacgg
     1981 cgtggccagg cgggcggcga tcatggtcgg ttcggggtcg cgtccggtca gggcggccag
     2041 cagcgggcgc aggaaaagct cggcgcagac cagggccgaa gcgggattgc cgggcaggcc
     2101 cagcacccgt cggccgtctg caagcgtccc gaaccaggtc ggcttgccgg ggcgaatggc
     2161 gatggtctcg actcgcaggc caaggcccag gcgcgccatc gccggcttga ccaggtcgtg
     2221 gtcgccgacc gaggccccgc cgacggtgac gatcaggtcg gcgtcgatgt cggccaccga
     2281 ggcggcgatg gcctgcgcat tgtcggcggc ggccgtcagg cgtgtcggga ggccgcccca
     2341 ggtctcgacc aaggccgcca gggcgatgga gccaggtcgc cgttggattc tcaggcacgt
     2401 cggatttcaa tttcacggca tctgacggaa ccactctaac cggaccatcg aaagact
//
LOCUS       DAOMEO010000439         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4569_length_2456_cov_2.070804, whole genome shotgun sequence.
ACCESSION   DAOMEO010000439 DAOMEO010000000
VERSION     DAOMEO010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4569_length_2456_cov_2.070804"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PLH31_10335"
     CDS             <1..154
                     /locus_tag="PLH31_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HQR89635.1"
                     /translation="LIRRDQERAEKIAAMQRLVDEAEESGVSSSSMDDILAAARRRLR
                     ADADGL"
     gene            138..425
                     /locus_tag="PLH31_10340"
     CDS             138..425
                     /locus_tag="PLH31_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR89636.1"
                     /translation="MRMAYRLSRKAEEDILDIYLIGAAAFGEDQAERYHAGLEQAFAF
                     LCDFPRAAPERAELGRASRVHPYKSHIIIYRLDGADIFIQRVRHGSEDWLP"
     gene            538..1185
                     /gene="plsY"
                     /locus_tag="PLH31_10345"
     CDS             538..1185
                     /gene="plsY"
                     /locus_tag="PLH31_10345"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQR89637.1"
                     /translation="MQDLAQAVYLTLGIAMVGGYLLGSIPFGLIATRLGGAGDIRQIG
                     SGNIGATNVLRSGRKDLAAITLIGDAGKGVLAVLLARWLTNDSAMIVALAGGSAFLGH
                     LFPVWLKFRGGKGVATFYGVLLAACWPVGVLAAITWLAMAALFRISSLAALTAAVLAA
                     PFALATDQPYAMLVLALFMAVLIFIRHRENIARLLKGEEPKIGKKKTAQTPPPAA"
     gene            1182..2279
                     /gene="dprA"
                     /locus_tag="PLH31_10350"
     CDS             1182..2279
                     /gene="dprA"
                     /locus_tag="PLH31_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQR89638.1"
                     /translation="MISGRLSDIQRLAWLRLARTETVGPVAFDHLLTRYGTPERALSA
                     LPDLSRRGGRATPLTPPPRDVIERELEAGAAMGARLLCGCEPDFPARLAALDPPPPVL
                     WALGRAELLPQPSVAIVGARIASAAGQRFARQLAAELGEAGHVIVSGMARGVDGAAHE
                     GAIRTGTVAVLGGGISDIYPPEHASLHARIAAEGCVVSESAPDRRAQAKDFPRRNRII
                     SGLSLGVVVVEAELKSGSLITARLAAEQGRDVFAVPGSPLDPRARGTNDLIRQGAILC
                     EGAEDVLRSLSGQIHLRERERGYDGEPEADVDFDALREQLAALLSPTGVSRDELVRAT
                     GAPTSAVMAALVELALAERAILLSGGMVAGV"
BASE COUNT          359 a          871 c          825 g          401 t
ORIGIN      
        1 cctgatccgc cgcgatcagg aacgcgccga aaagatcgcc gccatgcagc gcctggtcga
       61 tgaagccgag gaaagcgggg tcagttccag ctccatggac gacatcctgg cggcggcgcg
      121 ccgccggctg cgggccgatg cggatggcct ataggcttag ccgcaaagcc gaggaggata
      181 tcctcgacat ctacctcatc ggagcggcgg catttggtga agaccaggcg gagcgctatc
      241 acgctggctt ggagcaggct ttcgcgtttc tctgcgactt tccacgcgcc gctcccgaac
      301 gcgccgaact cggccgcgcg tcgcgcgtcc acccgtacaa gagccatatc atcatctacc
      361 gcctcgacgg ggcggacatc ttcatccagc gtgttcggca tggatccgaa gattggcttc
      421 cctgaagggc caggggctcg ccttttgccc cgaccctgag tctcctgcta caccttcgcc
      481 acaagcaact cttgcgcagc ggttgaaatc gcgcgcaact cgccttaggg tcgaaacgtg
      541 caagatctcg ctcaagccgt ctatctcacg ctcggtatcg ccatggtcgg gggctatctc
      601 ctgggctcga tcccgtttgg cctgatcgcc acccgcctgg gcggggccgg cgacatccgg
      661 cagatcgggt cgggcaatat cggcgcgacc aatgtgctgc gctcgggccg caaggatctg
      721 gcggccatca ccctgatcgg cgacgccggc aagggcgtgc tggccgtgct gctggcccgc
      781 tggctgacga acgacagcgc catgatcgtg gccctggccg gcggctcggc gttcctcggc
      841 catctgttcc cggtgtggct gaagttcagg ggcggcaagg gcgtggccac cttctatggc
      901 gtgctgctgg ccgcctgctg gcccgtgggc gtgctggccg cgatcacctg gctggccatg
      961 gccgccctgt tccgcatcag ctcgctggcc gccctgaccg ccgccgtcct ggccgcgccg
     1021 ttcgctctgg ccaccgacca gccctacgcc atgctggtgc tggccctgtt catggccgtg
     1081 ctgatcttca tccgccaccg cgagaacatc gcccgcctgc tgaagggcga ggagccgaag
     1141 atcggcaaga agaagacggc gcagacgccg cctccggccg cgtgatttcg ggccgcctct
     1201 cggatatcca gcgcctggcc tggcttcggc tggcgcgcac cgagacggtc ggccccgtcg
     1261 ccttcgacca tttgctgacc cgctacggca cgcccgagcg ggccctgagc gccctgcccg
     1321 atctgtcccg tcgcggcggc cgcgccacgc ccctgacccc gccgccgcgc gacgtgatcg
     1381 agcgcgaact ggaagccggc gcggccatgg gcgcccgcct gctgtgcggc tgtgagcccg
     1441 acttccccgc ccgcctggcc gcgctcgatc ccccgccgcc ggtgctgtgg gccctgggcc
     1501 gcgccgagct gctgccccag cccagcgtcg ccatcgtcgg cgcccgcatc gcctcggccg
     1561 ccggccagcg cttcgcccgc cagctggccg ccgagctggg cgaggccggt catgtgatcg
     1621 tcagcggcat ggcgcgcggc gttgacggcg ccgcccatga gggcgcgata cgcaccggaa
     1681 cggtcgccgt gctgggcggc gggatcagcg acatctatcc gcccgaacat gcctcgctcc
     1741 acgcccgcat cgccgccgaa ggctgcgtgg tcagtgaaag cgcgcccgac cgccgcgccc
     1801 aggccaagga cttcccgcgc cgcaaccgca tcatctcggg cctttcgctg ggcgtggtgg
     1861 tggtggaagc cgagctgaag tccggctcgc tgatcaccgc ccgcctggcc gccgaacagg
     1921 gccgtgacgt cttcgccgtg cctggctcgc cgctggaccc ccgcgctcga ggcaccaacg
     1981 acctgatccg ccagggcgcg atcctgtgtg aaggcgccga ggacgttctg cgctcgctgt
     2041 cgggccagat ccacctgcgc gaacgcgagc gtggctatga cggcgagcca gaggcggatg
     2101 tcgatttcga cgccctgcgc gaacagctgg ccgccctgct ctcgccaacg ggcgtatcgc
     2161 gcgacgagct ggtgcgggcg acaggcgccc cgacctcggc ggtgatggcg gcgctggtgg
     2221 agctagccct ggcggaacgg gcgatcctgc tgagcggcgg gatggtggct ggggtttgag
     2281 ccgccaaagt gcgacgaggc ttgcggccaa tactggatac cagtacgatg atgatcgagt
     2341 tcgaatggga tgatgagaag gcccgacgaa acttcgctaa gcacggcgtg acctttacag
     2401 ccgcaaagcg cgctgtgttg gatcccgatc gactggaatt gatcgacgac aggttc
//
LOCUS       DAOMEO010000440         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4575_length_2454_cov_2.455190, whole genome shotgun sequence.
ACCESSION   DAOMEO010000440 DAOMEO010000000
VERSION     DAOMEO010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_2454_cov_2.455190"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..467
                     /locus_tag="PLH31_10355"
     CDS             78..467
                     /locus_tag="PLH31_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HQR89639.1"
                     /translation="MSDQALADGLVAALKAAPAVTAILGQRIYARSPRVPTYPCLAVS
                     RTESRVVEGEVIEHLLTLTCVSKFGGPEEARAVVAAVRAVIHDARPTIAGRRLVNLRV
                     AYADVFPAAERELTLGIVRVRAVTEPL"
     gene            484..900
                     /locus_tag="PLH31_10360"
     CDS             484..900
                     /locus_tag="PLH31_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HQR89640.1"
                     /translation="MAAQAGKDILLKISDGAATPTFHTVAGLRARTISLNARTIDATD
                     SDSAGRWRELLAGAGVRACAVSGSGVFRDAASDAQVRQSFFDQSARVWRLVIPDFGQL
                     EGPFIVAALEYAGDHDGEAAFALSLASAGAVTFTAI"
     gene            1033..1308
                     /locus_tag="PLH31_10365"
     CDS             1033..1308
                     /locus_tag="PLH31_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTA-gp10 family protein"
                     /protein_id="HQR89641.1"
                     /translation="MPPPNPARGEVTVHLAGAPRRLCLTLGALARIEGALALTDWREL
                     PARMETLSARELLAVLAALIEGEPVDLSAVTIPEAVAAVAAALAASA"
     gene            1305..1487
                     /locus_tag="PLH31_10370"
     CDS             1305..1487
                     /locus_tag="PLH31_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HQR89642.1"
                     /translation="MNDWPDALRLAVRLNIPPEAFWRLSLREWRMLTQAPAAPVLTRP
                     TLDALIARFPDEETPP"
     gene            1484..1993
                     /locus_tag="PLH31_10375"
     CDS             1484..1993
                     /locus_tag="PLH31_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HQR89643.1"
                     /translation="MSGFDTDGLSSVPARAAEAAASLEALKAPAQRAASSIEEAFARA
                     GSSLARSLASAAADGEISLAELARAVIGAAGAAMKGGGLAEAVSGAFAGARADGGPVL
                     PGGAYLVGERGPEVFRPSCAGNVEPLGSGGVSVTVNVQGGDASGLARSDAHLAQALAR
                     AVSLGARGF"
     gene            complement(2117..>2454)
                     /locus_tag="PLH31_10380"
     CDS             complement(2117..>2454)
                     /locus_tag="PLH31_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HQR89644.1"
                     /translation="ALSLVSIWCSICIHTKRLHDMGQTGWWQALPWVFGPILVFGSAI
                     SIGVTAALAAVNNGEPDLAAITALGGFFISLFVAFAVWLAFTLWVGCSTGQPGENKYG
                     LAPTSAVAI"
BASE COUNT          334 a          914 c          863 g          343 t
ORIGIN      
        1 tacgcccatc gcgagaccgg cgagccctcg ctggccctgg tcgagccgtg gctggcccct
       61 taccgaaagg ctcgcctgtg agcgatcaag ccctggccga cggcctcgtc gccgccctca
      121 aggccgcgcc ggccgtcacc gccatcctcg gccagcggat ctatgcccgc agcccccgcg
      181 tgccgaccta tccctgcctg gccgtcagcc gcaccgagag ccgcgtggtc gagggcgagg
      241 tgatcgaaca cctgctgacc ctgacctgcg tctcgaaatt cggcggaccg gaggaggccc
      301 gcgccgtggt cgccgccgtc cgcgccgtga tccacgacgc ccgcccgacc atcgccggcc
      361 ggcgcctcgt caacctgcgc gtcgcctatg ccgacgtctt ccccgccgcc gagcgcgaac
      421 tgaccctcgg gatcgtgcgc gtgcgggcgg tgactgaacc cctctaatcc acggagcact
      481 cccatggccg ctcaagccgg caaggacatc ctgctgaaga tcagcgacgg ggccgccacc
      541 ccaaccttcc acactgtcgc gggcctgcgg gcgcgcacga tcagcctcaa tgccaggacc
      601 atcgacgcga ccgacagcga cagcgccggc cgctggcgcg agctgctggc cggggccggc
      661 gtgcgcgcct gcgcggtgtc gggctcgggc gtgttccgcg acgccgcttc cgacgcccag
      721 gtgcgccaga gcttcttcga ccagtcggcc cgcgtgtggc ggctggtgat cccggacttc
      781 ggccagctgg aaggaccgtt catcgtcgcg gcgctggaat atgccggcga ccacgacggc
      841 gaggcggcgt tcgcgctgtc gctggcgtcg gcgggggcgg tgacgttcac ggcgatttag
      901 agccgcgctg cccccctcag tcgctccgcg acagctcccc cagaggggga gcatctgctc
      961 ggcgcctcta gatcctcccc ctccggggga ggtggcccgc agggccggag ggggccatca
     1021 caggagacac ccatgccacc cccaaacccc gcccgaggcg aagtcactgt ccacctggcc
     1081 ggcgccccgc gtcgcctgtg cctgacgctc ggcgccctgg cccgcatcga aggcgccctg
     1141 gccctcactg actggcgcga actccccgcc cggatggaga ccctgtcggc ccgcgagctg
     1201 ctggccgtgc tggccgccct gatcgagggc gagccggtcg acctctccgc tgtcaccatc
     1261 cccgaagccg tcgccgctgt ggctgcggcg ctcgcggctt ccgcatgaac gactggcccg
     1321 acgccctgcg gctggccgtc cggctgaaca tcccgcccga ggccttctgg cgtctgtccc
     1381 tgcgcgaatg gcggatgctg acacaggcgc ccgccgcgcc ggtcctgacc cgtccgaccc
     1441 tggacgccct gatcgcccgc tttcccgacg aggagacccc gccatgagtg gtttcgacac
     1501 cgacggcctg tcgtccgtcc ccgcccgcgc cgccgaggcc gccgcgtcgc tggaggccct
     1561 gaaggcgccg gcccagcgcg cggcgtcgtc gatcgaggag gcgttcgcgc gggccggatc
     1621 cagcctggcc aggtccctgg ccagcgccgc cgccgacggc gagatctcgc tggctgaact
     1681 ggcgcgggcg gtgatcggcg cggcgggcgc ggcgatgaag ggcggcggtc tggccgaggc
     1741 ggtgtctggc gcgtttgccg gagcgcgagc ggacggcggc ccggtgctgc cgggcggcgc
     1801 ctatctggtc ggcgagcgtg ggccggaggt gtttcgacct tcctgcgccg ggaacgtgga
     1861 gccgctggga agcggcggcg tgtcagtcac ggtcaatgtg cagggcggcg atgcgtcagg
     1921 tcttgcgcgg tcggatgctc atctggccca ggccctggcg cgggcggtga gtttgggggc
     1981 gagagggttt tgaaccggct cctccccctc tgggggaggt ggcccagagg gccggagggg
     2041 ggcagcgcgg cctatgtcca gctaaccccc tcagtcgctc cgcgacagct cccccagagg
     2101 gggagcagcc tcgttcctag atcgccaccg ccgacgtcgg cgccaggccg tacttgttct
     2161 cgcccggctg gccggtgctg cagccgaccc acagggtgaa ggccagccac actgcgaagg
     2221 cgacgaacag cgagatgaag aagccaccca gggcggtgat cgccgccaga tcgggctcgc
     2281 cgttattgac cgccgccagg gccgccgtga cgccgatcga gatggccgag ccgaacacca
     2341 ggatcggacc gaacacccag ggcagggcct gccaccagcc ggtctgaccc atgtcgtgca
     2401 ggcgcttggt gtggatgcag atcgaacacc agatcgagac cagagacagg gccc
//
LOCUS       DAOMEO010000441         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4580_length_2453_cov_2.778148, whole genome shotgun sequence.
ACCESSION   DAOMEO010000441 DAOMEO010000000
VERSION     DAOMEO010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_2453_cov_2.778148"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..134)
                     /locus_tag="PLH31_10385"
     CDS             complement(12..134)
                     /locus_tag="PLH31_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89645.1"
                     /translation="MPPRQRPAPRLLSSMGLLFVLAAVAGLVGFVVITVISKFA"
     gene            347..583
                     /locus_tag="PLH31_10390"
     CDS             347..583
                     /locus_tag="PLH31_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89646.1"
                     /translation="MIDTAEEFGATLARSRKALGLTQRELALAINSGERFIVDLEAGK
                     PTAQLGKALAAAKAAGMVLIDSAEFSISPPRSTR"
     gene            583..1890
                     /locus_tag="PLH31_10395"
     CDS             583..1890
                     /locus_tag="PLH31_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HQR89647.1"
                     /translation="MVLPVFSDAIAVAVIDADADGPRFQYDPAWREAADAFPASLTMP
                     LDREVWGAEVATPWLMNLLPEGEPLRAMTRALGVSRDDILGLIAETGRDLAGALTIGA
                     PRPGDTPGYRQIGSPADLERILEELPAKPFLVGEDGVSMSLAGAQEKLPVALVGDQVA
                     VPVNGAASTRILKPDNSRLPGSVQNEALCMVLARRCGLSVAPVTTGLAGARSYLLVER
                     YDRILDGGLIRRLHQEDFCQALGRPPVAKYEHNQSGVRGPSLSDMFALVRTHMTAREI
                     TGLLDAAIFNIAIGNVDSHAKNYSVLLPSRRPQLAPLYDLMSGLVWPNITQNHAQDVG
                     GQRRGRHILGRHWRRMAEDCGLSRPATLRRVVLLAGRVVAEAAAAAEEVAAMPAGGGP
                     MLQTFVEAIRERAGLVLANVGREGGAADDEPLAENTGVTYGDV"
     gene            complement(1975..>2453)
                     /locus_tag="PLH31_10400"
     CDS             complement(1975..>2453)
                     /locus_tag="PLH31_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQR89648.1"
                     /translation="FTRIIKFAIATAMRQDEICQVTWGDLNVRSKMLLIRDRKDPRQK
                     QGNDQRIPLLSVSGYDPMDLIEEQRAIRANDDSRIFPYCGKSVSAAFTRACTALKIED
                     LHFHDTRHEGTSRLFEAGFSIEQVALVTGHKDWKMLRRYTHLRPEALHDIAARRVA"
BASE COUNT          426 a          777 c          796 g          454 t
ORIGIN      
        1 agcccggcgg cctaggcgaa cttgctgatc accgtgatga cgacgaagcc caccaagccc
       61 gcgaccgcgg ccaagacaaa gagcaggccc attgatgaca gcaggcgggg ggcggggcgt
      121 tggcgaggcg gcatgtcgcc atcctagcgg tttgccaggg ccaaagccat gtcggcgagg
      181 atggattgat cttaccgagc gggaagaatt tgcgtcatct gcagacatgg cgcatcgatc
      241 ttaccgatcg ggaagttgct aataagacgg cgttcttccc gatcggtaag attttggttg
      301 caaagagtgt tcaaggtgtc caatcttacc gaacgggaag attttcatga tcgacacagc
      361 cgaagagttt ggcgccaccc tggcgcggtc acgcaaggcc ttgggtctga cccagcgaga
      421 actcgcgctc gccatcaata gcggcgagcg cttcatcgtc gatctggagg ccggcaagcc
      481 aaccgcccag ctgggcaagg cgctggctgc ggccaaggcg gcgggcatgg tgctgatcga
      541 ctccgccgag ttctcgatct cgccgcccag gtcgacacgc tgatggttct tcccgtcttc
      601 agcgacgcca tcgccgtggc tgtcattgac gccgacgccg atgggccgcg cttccaatac
      661 gatccggcct ggcgtgaagc cgccgacgca tttcccgctt ccctcaccat gcccttggat
      721 cgagaggtct ggggggcgga ggtggcgacg ccttggctca tgaacctgtt gcccgagggc
      781 gagcccctgc gcgcgatgac gcgcgccttg ggtgtctcgc gcgacgacat tctgggtctg
      841 atcgcggaaa ccgggcgcga tctagccggc gcattgacca tcggcgctcc gcgccccggc
      901 gacacgccgg gctatcgtca gattggatcc cctgccgatc tcgagcgcat tttggaggaa
      961 cttccggcca agcctttcct agtgggggaa gacggcgtct ccatgagcct cgccggcgcg
     1021 caggagaagc tgccggtcgc cctcgtcggc gaccaagtcg ccgttcccgt caatggcgcg
     1081 gcctcgacgc ggattctcaa gccggacaat tcccgccttc cgggaagcgt gcaaaacgaa
     1141 gcgctgtgca tggtcctggc ccgacgctgc ggactttcgg tcgcgccggt caccacgggt
     1201 ctggccggag ccaggagtta tctcctggtc gaacgctatg atcgcatcct ggatggaggc
     1261 ctgatccgca ggcttcatca ggaggatttt tgccaagcgc tcggccgtcc gcccgtggcc
     1321 aagtacgagc acaaccaatc tggtgtccga gggccatcgc tgtccgacat gttcgcgctc
     1381 gtgcgaaccc acatgaccgc gcgagagatc accggactgc tggacgcggc gatcttcaac
     1441 atcgccatcg gtaacgtcga ttctcacgcc aagaactatt cggtcctcct gccgtcacga
     1501 cggccccaac tggcgccgct ctacgacctg atgtcgggct tggtttggcc caacatcacg
     1561 caaaaccacg cccaagatgt cggcgggcag cggcgcggcc gacacatcct tggccgccac
     1621 tggcgacgca tggccgaaga ttgcgggctc tcccgtcccg cgactttgcg gcgtgtcgtc
     1681 ttgcttgccg gacgcgttgt cgccgaggcg gccgcagctg ctgaggaggt cgcggccatg
     1741 ccggcggggg gcggcccgat gctgcagacg ttcgtggagg ccattcgcga acgcgccggc
     1801 ctggtcctgg ccaatgtcgg tcgggagggc ggagcggccg atgacgagcc cctcgccgaa
     1861 aacaccggcg tcacttacgg cgatgtctag ggtcactgac cagaggcctg ctgattacga
     1921 tcaagcctcc attccatgcc ccagcgacgc gccagcaatc tcgcctgtgg cgggctaagc
     1981 tacccggcga gcggcgatgt catgcagggc ttccggccgc agatgcgtgt agcggcgcag
     2041 catcttccaa tccttgtggc cggtgacgag cgcgacctgt tcgatcgaga agccggcctc
     2101 gaacaggcgg ctggtgcctt cgtgccgggt gtcatggaag tggaggtctt cgatcttcaa
     2161 cgccgtgcag gcgcgtgtga aagccgcact caccgacttg ccgcaatagg ggaagatccg
     2221 actgtcgtcg ttggcgcgga tcgcgcgctg ctcttcgatc agatccatcg gatcgtagcc
     2281 cgagaccgac aatagcggga tgcgttgatc gttgccctgc ttttgccgag ggtccttgcg
     2341 gtcgcggatc agcagcatct tggagcggac gttcagatcc ccccaggtta cctggcagat
     2401 ctcgtcttgg cgcatggcgg tggcgatcgc gaacttgatg atgcgcgtga acg
//
LOCUS       DAOMEO010000442         2450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4588_length_2450_cov_2.547808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000442 DAOMEO010000000
VERSION     DAOMEO010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4588_length_2450_cov_2.547808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLH31_10405"
     CDS             <1..415
                     /locus_tag="PLH31_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HQR89649.1"
                     /translation="ALHANHPRELTAAARAACAKIVDAGVPMISQTVLLAGINDDPET
                     LGALMRAFVETRIKPYYLHHHDYAPGTAHLRTSIAEGQALMKALRGAISGLCQPTYIL
                     DIPGGHGKVPVGPAYLGDQSVEDPQGQAHPYPPAG"
     gene            544..1422
                     /locus_tag="PLH31_10410"
     CDS             544..1422
                     /locus_tag="PLH31_10410"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQR89650.1"
                     /translation="MSEIKTVGVIGAGQMGCGIAHVCALAGYDVMLHDVTRERIDAGL
                     ALIDKNLARQVGRGIIDQAAMNAALARIKPAEGLEAVGQTDLAIEAATENEETKKAIF
                     RSLQPHLKPDTLLASNTSSISITRLASATDRPERFIGLHFMNPVPLMKLVEIIRGIAT
                     DVPTYERAVAFAQSLGKITSNAEDFPAFIVNRVLVPMINEAIYTLYEGVGTVDAIDTA
                     MKLGANHPMGPLELGDFIGLDTVLSIMNVLHEGLADSKYRPCPLLVKYVEAGWLGKKA
                     GRGFYDYRGETPVPTR"
     gene            1451..>2450
                     /locus_tag="PLH31_10415"
     CDS             1451..>2450
                     /locus_tag="PLH31_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQR89651.1"
                     /translation="MTDNQILRPVAASERHMSLDVLRGLGVMGILAVNAVAFAMPMEV
                     YMTPNLSPFPLTGAEGEAWWAVQTFFHFKFVTLFSMLFGVSILLVGGERSDKPRGALL
                     RRRLGWLLVFGLIHGLLIWFGDILLLYAVTGFVVLLFRSWKPRTLFIVSIIVILLGSA
                     LAVLPMMALQHAPPETRAEVLAQMAMGGPAEVARAIALVKSGLAGAMAENTEAWIKVQ
                     VMSVTIIIWRTGALMMLGMALYKWGFLTGRAPTWVYGALVVVGAAGLWVTGLESREKL
                     AINFAQPRSNGELQLGFEFLTLPITLGYASLAILLLKLDIARKLLNPLARLGQMAFT"
BASE COUNT          413 a          813 c          780 g          444 t
ORIGIN      
        1 ggcgctgcac gccaaccatc cccgcgaact gaccgccgcc gcccgcgccg cctgcgcgaa
       61 gatcgtcgat gcgggcgtcc cgatgatcag ccagaccgtg ctgctggccg ggatcaatga
      121 tgatcccgaa acgctgggcg ccctgatgcg cgccttcgtc gagacgcgga tcaagcccta
      181 ctatctgcac catcacgact acgcgcccgg caccgcccat ctgcgcacgt ccatcgcgga
      241 gggccaggcc ctgatgaagg ccctgcgcgg cgcgatctcg ggcctgtgtc agccgaccta
      301 cattctggac atcccgggcg gccacgggaa ggtgcccgtg ggacccgcct atctgggcga
      361 tcagagcgtc gaggatcctc aaggccaggc tcatccctac ccgcccgcgg gatagcgcga
      421 cacatccccg ctgcgtcgca gcaaagcctt ggcgagcccg tggtttctcg tgctagaaaa
      481 gccgccacaa tcaaggcgcg gattcacagt cctgtcgccg tatcaagcga aggcttggcg
      541 ttcatgagtg aaatcaagac ggtcggcgtt attggcgctg gccagatggg ctgtggcatc
      601 gcgcatgtct gcgccctcgc gggctatgac gttatgctgc acgacgtcac gcgcgagcgg
      661 atcgacgcgg gtctggcgct gatcgacaag aacctggcgc gtcaggtggg ccgcggcatc
      721 atcgaccaag ccgccatgaa cgctgccctg gcgcgaatca aacccgccga aggcctggaa
      781 gccgttggcc agaccgacct ggccattgag gcggcgaccg agaacgaaga gaccaagaag
      841 gccatcttcc gcagcctgca gccgcacctg aagccggata cactgctggc gtcgaacacc
      901 tcgtcgatct cgatcacccg cctggcctcg gccaccgacc gccccgagcg cttcatcggc
      961 ctgcacttca tgaaccccgt cccgctgatg aagctggtcg agatcattcg cggcatcgcc
     1021 accgacgttc cgacctatga acgggctgtg gccttcgccc agtcgctggg caagatcacg
     1081 tcgaacgccg aagacttccc ggccttcatc gtcaaccgcg tactggtgcc gatgatcaac
     1141 gaggcgatct ataccctgta cgagggcgtg ggtacggtcg acgccatcga caccgccatg
     1201 aagctgggcg ccaaccatcc gatgggcccg ctggaactgg gcgacttcat cggcctggac
     1261 accgtgctca gcatcatgaa cgtgctgcac gaaggtctgg ccgacagcaa ataccgcccc
     1321 tgcccgcttc tggtgaagta cgtggaagcg ggatggcttg gcaaaaaggc cgggcgcggg
     1381 ttctatgact atcggggcga gacgccggtt cccacgcgct aggcgtgcgg cgtctcctta
     1441 ggggacgccg atgaccgaca accagatctt gcggccggtg gcggccagcg agcgccatat
     1501 gagcctggac gtcctgcggg gcctgggcgt gatgggcata ttggcggtca acgccgtcgc
     1561 cttcgccatg ccgatggaag tctatatgac gcccaacctc tctcccttcc ccctgaccgg
     1621 ggcggaaggc gaggcctggt gggcggttca gacctttttc cacttcaagt tcgtcaccct
     1681 gttctcgatg ctgtttggcg tgtcgatcct gctggtcggc ggcgagcgca gcgacaagcc
     1741 gcgcggcgcc ctgctgcggc gacggctggg ctggctgctg gtgttcggcc tgatccatgg
     1801 cctgctgatc tggtttggcg acatcctgct gctctatgcg gtcaccggct ttgtggtttt
     1861 gctgttccgc tcctggaaac cgcgcaccct gtttatcgtg tcgatcatcg tcatcctgct
     1921 gggctcagcc ctggcggtgc tgccgatgat ggcgctgcaa cacgccccgc ccgagacccg
     1981 ggccgaggtg ctggcccaga tggccatggg cggccctgct gaagtggcgc gcgcgatcgc
     2041 cctggtgaaa tcgggcctgg ccggggccat ggccgagaac accgaagcct ggatcaaggt
     2101 tcaggtgatg agcgtgacca tcatcatctg gcgcaccggc gccctgatga tgctgggcat
     2161 ggcgctctac aagtggggct tcctgaccgg ccgcgcgccg acctgggtct atggcgctct
     2221 ggtggtcgtt ggcgccgcag gcctgtgggt gacgggcctg gagtcacgcg agaagctggc
     2281 gatcaatttt gctcagcccc gttcgaacgg cgaactgcaa ctgggctttg agttcctgac
     2341 cctgccgatc acactgggct atgcctccct ggcgatcctg ctgctgaagc tggatatcgc
     2401 tcgcaagctg ctcaacccgc tggcccgcct gggacagatg gccttcacca
//
LOCUS       DAOMEO010000443         2447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4595_length_2447_cov_2.425167, whole genome shotgun sequence.
ACCESSION   DAOMEO010000443 DAOMEO010000000
VERSION     DAOMEO010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_2447_cov_2.425167"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLH31_10420"
     CDS             complement(<1..653)
                     /locus_tag="PLH31_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR89652.1"
                     /translation="MPIGPFKTKPAGPRPLSLALQGGGSHGAFEWGVLDRLLEEDDIE
                     IKAVTAASAGAMNAVCLAQGLLDNGRDGARERLELFWKAVNTAGGRNVFGDTSIWTSA
                     FSGGSDWLKSLPGWRMAETLAMSMSPYEFNPFNLNPLHDVLEDQINFQALREGSPVKL
                     YIAATAVRLSKSKVFRETEVTSKHIMASACLPHLFHAVEIDGEPYWDGGFLANPPLWP
                     "
     gene            complement(667..>1192)
                     /locus_tag="PLH31_10425"
     CDS             complement(667..>1192)
                     /locus_tag="PLH31_10425"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HQR89653.1"
                     /translation="LINNAGVQHVAPVEDFPTEKWDQIIAINLTSAFHAIKAAVPIMK
                     AQGRGRIVNIASAHGLVASPFKSAYVAAKHGIMGLTKTVGLEVAQHGITCNAICPGFV
                     KTPLVEAQIADQARARGISEEAVMKDVILASQPTKQFVTFDQLSGMLLYLVSDAGASA
                     NGSAFQIDGGWTAQ"
     assembly_gap    1193..1202
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1203..1472)
                     /locus_tag="PLH31_10430"
     CDS             complement(<1203..1472)
                     /locus_tag="PLH31_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89654.1"
                     /translation="MAVDFGLQGQVAIVTGSTSGIGHALADALAAQGVNIVLNGLGEM
                     NAIERTRAELAEKHGVEVLYNGADMTKPDQIIDMVRAAKAEFGELD"
     gene            complement(1573..2340)
                     /gene="yaaA"
                     /locus_tag="PLH31_10435"
     CDS             complement(1573..2340)
                     /gene="yaaA"
                     /locus_tag="PLH31_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HQR89655.1"
                     /translation="MLMVISPAKSLDFTAPDQVLPLTTPELKGRIAELAKVTRKLSAA
                     DLRRLMHISEKLASLNRERFQAFDPEVEDGLQAVIAFNGDVYAGLAARELDRPALEWA
                     QDHLRILSGLYGVLRPLDALQPYRLEMGTRLKTKRGANLYDFWGETIAKTLNDAAEGH
                     ADKTLVNLASQEYCGAVDAKALKLPVLTCHFKEEKAGLLKVLGFFAKKARGRMARYII
                     DNRIEQAKDLKAFDLDGYAFQPKLSTDTDWVFARPQP"
BASE COUNT          406 a          779 c          810 g          442 t
ORIGIN      
        1 ggccatagcg gcggattggc caggaaacca ccgtcccaat agggctcgcc gtcgatctcc
       61 accgcatgga acaggtgcgg caggcaggcg ctggccatga tgtgtttcga ggtcacctcg
      121 gtctcgcgaa acaccttgga cttgctgagc cgcacggcgg tggcggcgat gtacagcttg
      181 accggcgagc cttcgcgcag ggcctggaaa ttgatctggt cctccagcac gtcgtgcagg
      241 gggttcaggt tgaacgggtt gaactcgtag gggctcatcg acatggccag ggtctcggcc
      301 atacgccagc cgggcaggct tttcagccag tccgagccgc cggaaaaggc gctggtccag
      361 atgctggtgt cgccgaacac gttgcggccg ccggcggtat tgaccgcctt ccagaacagc
      421 tcaagccgct cgcgggcgcc gtcgcggcca ttgtccagaa ggccctgggc cagacacacg
      481 gcgttcatgg cgccggccga ggcggccgtg accgccttga tctcgatatc gtcttcctcg
      541 agcaggcggt ccagcacgcc ccactcgaac gccccgtgcg agccgccgcc ctgcagggcc
      601 aggctcagcg gccggggccc ggcgggcttg gtcttgaagg ggccgatggg catggcccgc
      661 ttccctctat tgcgcggtcc agccgccgtc gatctggaag gccgagccgt tggccgaggc
      721 cccggcgtcg gacaccagat acagcaacat gccagacagc tggtcgaaag tgacaaattg
      781 cttggtcggc tggctggcca ggatgacgtc cttcatgacc gcctcctcgc tgatgccgcg
      841 cgccctggcc tggtcggcga tctgggcctc gaccagcggg gtcttcacaa agcccgggca
      901 gatggcgttg cacgtgatgc cgtgctgcgc gacctccagc ccgaccgtct tggtcaggcc
      961 catgatcccg tgcttggcgg ccacataggc cgacttgaac ggcgaggcga ccaggccatg
     1021 cgcggaagcg atattgacga tccgtccgcg cccctgcgcc ttcatgatcg gcacggcggc
     1081 cttgatggcg tggaaggccg aggtcaggtt gatggcgatg atctggtccc acttctcggt
     1141 ggggaaatcc tcgaccgggg ccacgtgctg gacgccggca ttgttgatca ggnnnnnnnn
     1201 nngtccagct caccgaactc ggccttggcc gccctgacca tgtcgatgat ctggtccggc
     1261 ttggtcatgt cggcgccgtt gtacagcacc tcgacaccgt gcttttccgc cagctcggcg
     1321 cgggtgcgct cgatggcgtt catctcgccc agaccgttca ggacaatatt gacgccctgc
     1381 gccgcgagcg catccgccaa ggcgtgaccg atgccgctgg tcgagccggt gacgatggcg
     1441 acctgcccct gcaaaccgaa gtctacggcc atgaacacgc tccctgatgc attgcccaag
     1501 tctatgctgc aacgcagcat atagctcgcg attgcgaacg gcgagctttt ataaattcat
     1561 cactaaatga attcagggct gaggacgagc aaaaacccag tcggtgtctg tggagagctt
     1621 gggctggaag gcgtagccat ccagatcgaa ggccttcagg tccttggcct gctcgatccg
     1681 gttatcgatg atgtagcgcg ccatccggcc ccgggccttc ttggcgaaga agcccagcac
     1741 cttcagcagg cccgccttct cctccttgaa gtggcaggtc agcaccggca gcttcagggc
     1801 cttggcgtcg acggcgccgc aatattcctg gctggccagg ttcaccaggg tcttgtcggc
     1861 atgaccctcg gcggcgtcgt tgagggtctt ggcgatcgtc tcgccccaga aatcgtacag
     1921 attggccccg cgcttggtct tcagtcgcgt acccatctcc aggcgatagg gctgcagggc
     1981 gtccagcggc cgcagcacgc cgtacaggcc tgacaggatc cgcaggtggt cctgggccca
     2041 ctccagggcc ggccggtcca gctcgcgcgc cgccaggccc gcatagacgt cgccgttgaa
     2101 ggcgatcacc gcctgcaggc cgtcctcgac ctcgggatcg aaggcctgga agcgctcgcg
     2161 attcagcgag gccagcttct cggagatgtg catcagccgg cgcaggtcgg cggcgctcag
     2221 cttgcgtgtc accttggcca gctcggcgat cctgcccttc agctcgggcg tcgtcagggg
     2281 cagcacctga tcgggcgcgg tgaagtccag cgacttggcg ggggagatga ccatcaacat
     2341 gagcggggag atagagggtc ctgaggggga tcgctagcgg gaggttggcg attggtgggc
     2401 gcggagcctc cttgcctctt tgatggagga agggagaaga cacggct
//
LOCUS       DAOMEO010000444         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4598_length_2446_cov_2.372647, whole genome shotgun sequence.
ACCESSION   DAOMEO010000444 DAOMEO010000000
VERSION     DAOMEO010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_2446_cov_2.372647"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLH31_10440"
     CDS             <1..525
                     /locus_tag="PLH31_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraI"
                     /protein_id="HQR89656.1"
                     /translation="AATVHRKPDVGRLGRKPPPQSQHRLRALSELGVVRIAGGSEVLL
                     PRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYIAEREAKRLKGFDIP
                     KHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARRLTRIAVGDAVSI
                     TAKGSIKTSKGRSR"
     gene            522..2435
                     /locus_tag="PLH31_10445"
     CDS             522..2435
                     /locus_tag="PLH31_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQR89657.1"
                     /translation="MKIKMNNAVGPQVRSAKPKPSKLLPVLGAASMVGGLQAATQFFA
                     HTFAYHATLGPNVGHVYAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVA
                     KVVTSNSSKANEYLHGSARWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGW
                     QDKDGNFFYLRHSGPEHVLTYAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTA
                     GWRQKHAKNKVLRFEPASTSGGVCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLD
                     SHWQKTAFALLVGVILHALYKAKDDGGTATLPSVDAMLADPNRDIGELWMEMATYGHV
                     DGQNHHAIGSAARDMMDRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSDFRIKQLMH
                     EDDPVSLYIVTQPNDKARLRPLVRVMVNMIVRLLADKMDFEGGRPVAHYKHRLLMMLD
                     EFPSLGKLEIMQESLAFVAGYGIKCYLICQDINQLKSRETGYGHDESITSNCHVQNAY
                     PPNRVETAEHLSRLTGQTTVVKEQITTSGRRTAAMLGQVSRTYQEVQRPLLTPDECLR
                     MPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPLYFKDPVFSARAAIPAPKVSDRLRA
                     VAQAETEGEGITI"
BASE COUNT          491 a          800 c          799 g          356 t
ORIGIN      
        1 gccgccaccg tccaccggaa gcccgacgtt ggacgccttg gaagaaaacc gccgccccaa
       61 agccagcacc gtttgcgagc actgtccgaa ctcggtgtgg ttcgcatcgc cggcggaagt
      121 gaagtgctat tgccgcgtga tgttcctcgt cacgtggagc agcaaggaac ccaaccagat
      181 cacgcactgc gacggggaat ttctcggcca ggaacaggag taggccagac gccgccgggc
      241 gtcgccgccg ccgacaagta catcgccgaa cgcgaggcaa agcgcctaaa aggtttcgat
      301 ataccgaagc attcccgata tactgccggt gatggtgcgc tcacgttcca gggcaccagg
      361 actatcgagg gccaggcgct ggccctcttg aaacgaggtg atgaggtcat ggttatgccc
      421 atcgaccagg ccacggcccg gcgcttgacg cgcatcgcgg tcggcgatgc cgtctcgatc
      481 accgcgaaag gctccatcaa aacgtcgaaa ggaaggagta gatgaagatc aagatgaaca
      541 acgcggtagg gccgcaagtc cgatccgcca agccgaagcc gagcaagctg ctgccggtgc
      601 tgggcgcggc ctcgatggtc ggcggcctcc aggccgcgac gcagttcttc gcccacacgt
      661 tcgcctatca cgcgaccctc ggccccaacg tgggccacgt gtacgcgccc tggtcgatcc
      721 tgcactggac gtacaagtgg tacagccagt acccggacga gatcatgaag gccggcagca
      781 tggggatgct ggtttcgacc gtgggcctgc tgggcgtggc cgtggcgaag gtcgtcacgt
      841 ccaacagctc gaaggcgaac gagtacctgc acggctcggc ccgctgggcc gagaagaagg
      901 acattcaggc ggccggcctg ctgccacgcg agcgcaacgt cctggagatc gtcaccggca
      961 aggccgcacc cacggctacc ggcgtgtacg tgggcggctg gcaggacaag gacggcaatt
     1021 tcttctacct gcggcacagc ggccccgagc acgtgctgac ctacgcgccc acgcgctcgg
     1081 gcaagggcgt cggcctggtg gtgcccacgc tgctgtcgtg gggcgcgagc agcgtcatca
     1141 ccgacttgaa gggcgagttg tgggccttga ccgccggctg gcggcagaag cacgccaaga
     1201 acaaggtgct gcgcttcgag ccggccagca cctcgggcgg cgtctgctgg aatccgctgg
     1261 acgaaatccg cctcggcacc gaatacgagg tgggtgacgt gcagaacctt gccacgctga
     1321 tcgttgaccc ggacggcaag ggcctggact cgcactggca gaagaccgcc ttcgcgctcc
     1381 tggtcggcgt gatcctgcac gcgctgtaca aggccaagga cgatggcggc accgccacgc
     1441 tgccgtccgt cgatgcgatg ctggccgacc cgaaccggga cattggcgag ctttggatgg
     1501 aaatggcgac ctacgggcac gtagacggcc agaaccatca cgccatcggc tcggcggccc
     1561 gcgacatgat ggatcggccc gaggaagaag ccggatcggt gctgtccacg gccaagtcgt
     1621 acctggccct gtaccgcgac cccgtggtgg cccgcaacgt cagccggtcg gacttccgca
     1681 tcaagcagtt gatgcacgag gacgacccgg taagcctcta catcgtcacc cagcccaacg
     1741 ataaggcccg cctgcggccg ctggtgcgcg tcatggtgaa catgatcgtg cgcctgctgg
     1801 ccgacaagat ggacttcgag ggcggccggc cggtggcgca ctacaagcac cggctcttga
     1861 tgatgctgga cgagttcccg agcctcggga agctggagat catgcaagag tcgctggcct
     1921 tcgtggccgg ctacggcatc aagtgctacc tcatctgcca ggacattaac cagctcaaga
     1981 gccgcgagac gggctacggc cacgacgaaa gcatcacgtc gaactgccac gtgcagaacg
     2041 cctacccgcc caaccgcgtc gagacggccg aacacctgtc ccgcctcacc gggcagacca
     2101 ccgtggtgaa ggagcagatc acgaccagcg gccgccgcac ggcggcgatg ctgggccagg
     2161 tgtcgcgcac ttaccaggaa gtgcagcggc ccttgctgac gcccgacgaa tgcctgcgca
     2221 tgccggggcc aaagaagaac gcccagggcg agatcgaaga agccggcgac atggtgatct
     2281 acgtcgcggg ctacccggcg atctacggca agcaacccct gtacttcaaa gacccagtgt
     2341 tctcggcacg cgccgcgatc ccggcgccca aggtcagcga ccgcctgcgc gccgtcgcac
     2401 aggccgagac ggaaggggag ggcatcacga tatgagccgc atcctg
//
LOCUS       DAOMEO010000445         2440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4610_length_2440_cov_2.482180, whole genome shotgun sequence.
ACCESSION   DAOMEO010000445 DAOMEO010000000
VERSION     DAOMEO010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_2440_cov_2.482180"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLH31_10450"
     CDS             <1..913
                     /locus_tag="PLH31_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HQR89658.1"
                     /translation="VADWPAVAAGRASDEAMADYLRGLDAGKPVAILHGEPEIGGRFF
                     YTDDMSGLNFQRRNGPLGEALDRLLALKEHTAPSALAVQSTPTAAALPGFAQANPQPL
                     LDASVGPRIWIGNAITTATHFDMNYNIACAVAGRRRFTLFPPDQLKTLYLGPLEFTPA
                     GPPVSMVDIAAPDLERYPRFAEAWATAQVAELEPGDAIYIPYFWFHNVESLDPFTVLV
                     NYWWSETPAVLSRPLEVLLHALASLRDLPPREREIWRGMFEHFVFQAHGDPVAHLPPE
                     RRGSLGEMTPEHLARIRAILLRALSQP"
     gene            960..>2440
                     /locus_tag="PLH31_10455"
     CDS             960..>2440
                     /locus_tag="PLH31_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HQR89659.1"
                     /translation="MAYRSLREFIDVLEAQGELVRVTEPVSTVLEMTEIQTRLLATGG
                     PAVLFENVILPDGSRSPMPALANLFGTVKRVAMGVTLGGEPRTTAGELREVGELLAFL
                     RQPQPPKGLKDALDLLPLAKTVMAMRPGTVKKAPVQEIVLTGDDIDLSKLPVQTCWPG
                     EPAPLITWPLIVTKGPSKDREDDFNLGIYRMQVLGKDRCIMRWLAHRGGAQHYARHKQ
                     AGRREPLPACAVLGADPGTILAAVTPVPDTLSEYQFAGLLRGAKVDLVPAKTVPLMVP
                     AHAEIVLEGHVLLDEYADEGPYGDHTGYYNSVEKFPVFQVTAITMRKNPIYLTTFTGR
                     PPDEPSVLGEALNEVFIPLLRQQFPEIVDFWLPPEGCSYRIAVVSMKKAYPGHAKRVM
                     LGVWSYLRQFMYTKWVIVVDHDINARDWKDVMWAISTKMDPARDITVIEHTPIDYLDF
                     ASPESGLGSKIGLDATDKLPPETHREWGQEIRMDQAVIDKVSD"
BASE COUNT          410 a          865 c          777 g          388 t
ORIGIN      
        1 ggtcgccgac tggcccgccg tggcggcggg gcgcgcctcg gacgaggcca tggccgacta
       61 tctgcgcggg ctcgacgccg gcaagccggt cgccatcctg catggcgagc ccgagatcgg
      121 tgggcggttc ttctataccg acgacatgag cggtctgaac ttccagcgcc gcaacggccc
      181 gctgggtgag gcgctggatc gattgctggc gctgaaagaa cacaccgcgc cgtcggccct
      241 ggccgtgcag tcgacgccaa cggcggcggc tctgccgggc tttgcccagg ccaatccgca
      301 gccgctgctc gacgcctcgg tcgggccgcg catctggatc ggcaacgcca ttaccaccgc
      361 gacccatttc gacatgaact acaacatcgc ctgcgcggtg gcgggccggc gacgtttcac
      421 cctgtttccg ccggatcagt tgaagaccct ctatctcggc ccgctggagt tcacgcctgc
      481 ggggccgccc gtcagcatgg tcgacatcgc cgcgcccgat ctggagcgct atccgcgttt
      541 cgccgaagcc tgggcgacgg cgcaggtggc cgaactggag ccgggggacg cgatctacat
      601 cccctatttc tggttccaca atgtcgagtc gctcgacccc ttcaccgtgc tggtgaacta
      661 ctggtggagc gagaccccgg ccgtcctgag ccggcccctg gaggtgctgc tgcacgccct
      721 ggccagcctg cgcgacctgc cgccccgcga gcgggagatc tggcgcggca tgttcgaaca
      781 cttcgttttc caggcccacg gcgacccggt agcgcacctc ccgcccgagc gacgcgggtc
      841 tctgggggag atgacgcccg agcatctggc caggatccgc gcgatcctgc tgcgcgccct
      901 cagccagcca tagccagacc cacaaaatcc gttaggttcc gcgcgttcca ggagtcccca
      961 tggcctaccg ttccctgcgt gaattcatcg atgttcttga ggcccagggc gagctggtcc
     1021 gggtgacgga gccggtctcc accgttctgg agatgaccga gatccagaca cgccttctgg
     1081 ccaccggcgg tccggcggtc ctgttcgaga acgtcatcct gcccgacggc tcgcgctcgc
     1141 cgatgccggc gctcgccaat ctgttcggca cggtcaagcg cgtggccatg ggcgtcaccc
     1201 tgggcggcga gccgcgcacc accgccggcg aactgcgcga agtgggcgag ctgctggcct
     1261 tcctgcgcca gccccagccg cccaagggcc tgaaagacgc gctggacctg ctgcccctgg
     1321 ccaagaccgt gatggccatg cggccgggca cggtgaaaaa ggcccccgtg caggaaatcg
     1381 tcctgaccgg cgacgacatc gacctgtcca agctgcccgt ccagacctgc tggcccggcg
     1441 aaccagcgcc gctgatcacc tggccgctga tcgtcaccaa ggggccgagc aaggatcgcg
     1501 aggacgactt caacctgggc atctaccgca tgcaggtgct gggcaaggac cggtgcatca
     1561 tgcgctggct ggcccatcgc ggcggggccc agcactatgc ccgtcacaaa caggctggcc
     1621 gcagagagcc cctgcccgcc tgcgccgtgc tgggcgccga tcccggcacc atcctggccg
     1681 ccgtaacgcc cgtgcccgac accctgtcgg agtaccagtt cgccggtctg ctgcgcggcg
     1741 ccaaggtcga tctcgtgccc gccaagaccg taccgctgat ggtcccggcc cacgccgaga
     1801 tcgtgctgga aggccacgtc ctgctcgacg agtacgcgga cgaaggtccc tatggcgacc
     1861 acaccggtta ctacaacagc gtcgagaagt tcccggtctt ccaggtgacg gcgatcacca
     1921 tgcgcaagaa cccgatctac ctgaccacct tcaccggccg gccgccggat gagccctcgg
     1981 tgctgggcga agcgctcaat gaggtgttca tcccgctgct gcgccagcag ttccccgaga
     2041 tcgtcgattt ctggctaccg cccgagggct gcagctaccg catcgccgtg gtgtcgatga
     2101 agaaggccta tcccggccac gccaagcgcg tgatgctggg cgtctggagc tatctgcgcc
     2161 agttcatgta caccaagtgg gtgatcgtcg tggaccacga catcaacgcc cgcgactgga
     2221 aggacgtgat gtgggcgatc tccaccaaga tggacccggc gcgggacatc acggtgatcg
     2281 agcacacacc catcgactat ctcgacttcg ccagccccga aagcggcctg ggatcgaaga
     2341 tcggcctgga cgccaccgac aagctgccgc ctgagaccca tcgcgaatgg ggccaggaga
     2401 tccgcatgga tcaggcggtc atcgacaagg tcagcgacat
//
LOCUS       DAOMEO010000446         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4615_length_2438_cov_2.553504, whole genome shotgun sequence.
ACCESSION   DAOMEO010000446 DAOMEO010000000
VERSION     DAOMEO010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_2438_cov_2.553504"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLH31_10460"
     CDS             <1..377
                     /locus_tag="PLH31_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR89660.1"
                     /translation="ATAGADASIVNIVDQRVWRPNPQFFSYSLSKAGLWWATQTLAQA
                     LAPRIRVNAIGPGPTLPNIHQAPGEFEAEAAGTLLQRRATPDDVTAALRYLIDARAVT
                     GQMIAVDAGQHLGWLTPDIVAP"
     gene            391..798
                     /gene="folB"
                     /locus_tag="PLH31_10465"
     CDS             391..798
                     /gene="folB"
                     /locus_tag="PLH31_10465"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQR89661.1"
                     /translation="MAASPLAETAADTAPVAKVIVTKVFVRALKVDAWIGVYDHEHGA
                     RQPLVIDVELDVAASHCERLGDTVNYETIGQAARAIADEGHIDLVETFAERLAQACFA
                     DARVTRARVRVEKPLALAPHAAAAGVEITAVRA"
     gene            798..1199
                     /locus_tag="PLH31_10470"
     CDS             798..1199
                     /locus_tag="PLH31_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89662.1"
                     /translation="MSVSHSARLAPFQPETVWTLKDGTLVETRGVRVRGFPLSALTRF
                     RLAAAPSGGHRRVLQLSFGRARVIIIAQSYVGPGRVEDRLASFSAFARILAHHAADAA
                     PAARFNLASWPCCCRRWAPAWPGWASTWPRG"
     gene            1414..1776
                     /locus_tag="PLH31_10475"
     CDS             1414..1776
                     /locus_tag="PLH31_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQR89663.1"
                     /translation="MTDPAPAVPPALQDDKTLPIVVYVLHLAGLVTGITSLIAVVLAY
                     VSRKNAPEWIQSHYEFAIRTFWLALLFAIVGAVLSVVGIGVVILAAIGVWVAVRCIVG
                     LSYLLKGQPYPTPKNWML"
     gene            1780..>2082
                     /locus_tag="PLH31_10480"
     CDS             1780..>2082
                     /locus_tag="PLH31_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89664.1"
                     /translation="MTDTSAPSLPPVVRAEEDKVLPAVVYGLYLLGFASCAATTIVGL
                     IVAYANRSTAGPINESHYTFAVRSFWLTIGWFLIGLVLFIVGLPLSLLIVGIPVVAA"
     assembly_gap    2083..2092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2093..2160
                     /locus_tag="PLH31_10485"
     CDS             <2093..2160
                     /locus_tag="PLH31_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89665.1"
                     /translation="GVGIYYLTRGEAYPRPRTWLI"
     gene            2300..>2438
                     /locus_tag="PLH31_10490"
     CDS             2300..>2438
                     /locus_tag="PLH31_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89666.1"
                     /translation="MLARIVGIILGTILATSGYVVAGWGQLGSWAAHLDLGPFEPHRP
                     LA"
BASE COUNT          336 a          880 c          780 g          432 t
ORIGIN      
        1 aggcgacggc cggggctgac gcctcgatcg tcaatatcgt cgaccagcgg gtctggcggc
       61 ccaatccgca gttcttcagc tacagcctga gcaaggccgg cctgtggtgg gcgacccaga
      121 ccctggccca ggccctggcc ccgcgcatcc gcgtcaacgc catcggcccc ggccccaccc
      181 tgcccaacat ccaccaggcc cccggcgagt tcgaggccga ggccgccggc accctgttgc
      241 agcgccgcgc cacccccgac gacgtcaccg ccgccctgcg ctatctgatt gacgcaaggg
      301 cggtcacggg ccagatgatc gcggtggacg ccggacagca tctgggctgg ctgacgcccg
      361 acatcgtcgc accttaagaa agggcgccgc ttggccgcct cccccctcgc cgaaaccgcc
      421 gcagacaccg cgcccgtcgc caaggtcatc gtgaccaagg tcttcgtccg cgcgctcaag
      481 gtggacgcct ggatcggggt ctatgaccac gagcacggcg cccgccagcc gctggtcatc
      541 gatgtcgagc tggacgtcgc cgccagccac tgcgagcgcc tgggcgacac cgtcaattac
      601 gagaccatcg gccaggccgc ccgcgccatc gccgacgagg gccacatcga cctggtcgaa
      661 accttcgccg aacgcctggc ccaggcctgt ttcgccgacg cccgcgtcac tcgcgcccgg
      721 gtgcgggtgg aaaagcccct ggccctggcc ccgcacgccg ccgccgccgg cgtggaaatc
      781 accgccgtcc gggcctgatg tctgtctccc acagcgcccg cctcgcgccg tttcagcccg
      841 aaacggtctg gacgctgaag gacgggacgc tggtcgagac gcgcggcgtg cgcgtgcgcg
      901 gtttcccctt gagcgccctg acgcgctttc gcctcgccgc cgcgcccagc ggcggccatc
      961 gccgcgtgct gcaactgagc tttggccgcg cccgggtgat catcatcgcc cagagctatg
     1021 taggccccgg ccgcgtcgag gaccgactgg ccagtttctc cgccttcgcc cgcatcctgg
     1081 cgcatcacgc cgctgacgcc gcgcccgccg cgcggttcaa cctggcgtcg tggccctgct
     1141 gctgtcgtcg ctgggcgccg gcatggccgg ggtgggcgtc gacatggccg cgcggatgag
     1201 ctttgtgctg atcctgctgc tggcggcgct gccgtggctg tcgcgcggag acggctttga
     1261 cccgcgcgat ccgcccaggg acctgctgcc ctagagtatt ttccgacgcg atggcgtctt
     1321 cgagtctctg cgggttacgg gaccttcatt tgcatcgatg accgataagg acgatccttg
     1381 ggacaacatc gttttccaag gctcagcttc atcgtgaccg atcccgcccc cgccgtcccg
     1441 cccgccctgc aggacgacaa gaccctgccc atcgtcgtct atgtcctgca tctggcgggt
     1501 ctggtgacgg gcattacctc gctgatcgcc gtggtgctgg cctatgtcag ccgcaagaat
     1561 gcgccggagt ggatccagag ccactacgag ttcgccattc gcaccttctg gctggccctg
     1621 ctgttcgcca ttgtcggcgc ggtgctgagc gtggtcggca ttggcgttgt catcctggcg
     1681 gcgatcggcg tgtgggtcgc ggtgcgctgc atcgtgggcc tgtcttacct gctcaagggt
     1741 cagccctatc ccacccccaa gaactggatg ctctaagcca tgaccgacac ctccgcaccc
     1801 agcctgcctc ccgttgtgcg cgccgaagag gacaaggtcc tgccggccgt cgtctacggc
     1861 ctgtatctgc ttggcttcgc cagctgcgcc gccaccacca tcgtcggcct gatcgtcgcc
     1921 tacgccaacc gttcgacggc gggaccgatc aacgagagcc actacacctt cgccgtccgc
     1981 agcttctggc tgaccatcgg ctggttcctg atcggcctgg tgctgttcat cgtgggcctg
     2041 ccgctgtcgc tgctgatcgt cggcatcccg gtggtggcgg ccnnnnnnnn nngcggcgtc
     2101 gggatctact acctgacgcg cggcgaagcc tatccgcgcc cccggacatg gctgatttag
     2161 aggcgccttt ggcggtcggc gcccgtagaa gggtcgtccg atcgcgcctg aatcccccac
     2221 cggttgagac ggctcgacgt cgcgggcccg ctcggctgac ctatggctcc tggcgaatgg
     2281 acgtcttgga gggggcggca tgctggctcg tatcgtaggg atcatcttgg ggacgatcct
     2341 ggcgacgtcg ggatatgtgg tcgcaggctg ggggcagctg ggaagctggg ccgcgcacct
     2401 tgatcttgga cctttcgagc cgcatcgacc attggccg
//
LOCUS       DAOMEO010000447         2438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4616_length_2438_cov_2.489299, whole genome shotgun sequence.
ACCESSION   DAOMEO010000447 DAOMEO010000000
VERSION     DAOMEO010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_2438_cov_2.489299"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>632)
                     /locus_tag="PLH31_10495"
     CDS             complement(<1..>632)
                     /locus_tag="PLH31_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275049.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR89667.1"
                     /translation="LAATIQDHAVKAALKPYTLSGPYGHLLDANQNALKPAAWQTFEM
                     AELMASKSALAPVLTYIFRSLERRFDGRPTLLVLDEAWLFLDQGAFAAKIREWLKTLR
                     KFNVAVVFATQSLADVARSSIAPALIESCPTRIFLPNPDAQTPQITALYQDFGLNPQQ
                     VRIIANATPKREYYYQSTSGNRLFELGLGGVSLAAVGSSSPGDQQLISQL"
     assembly_gap    633..642
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<643..>2178)
                     /locus_tag="PLH31_10500"
     CDS             complement(<643..>2178)
                     /locus_tag="PLH31_10500"
                     /inference="COORDINATES: protein motif:HMM:NF010447.0"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR89668.1"
                     /translation="ALRRLGSRWCLHIEAVRAPSQTYPTSQFPDPVSDLVDEERREGF
                     EAQERHFESRYFLTFTYLPPEEAISTAESLLLENAPSGRGAEGMYRAALSDFLSTVHQ
                     IADILTAIMPEVAELTDDETLTYLHSCISTKRHPVATPETPAYLDAFLTDDDFQGGLL
                     PRLGGQYLRTLSVRAYPTTSCPGLLDRLNELGISYRWVCRYLPLDKEDARKAVTTVRK
                     RWFAKRKGVMALLKEAITREPSLLEDPDAAAKSVDADAALAILGGDYASIGYFTPTVT
                     LMDADPDRLANRVREVEGAINRAGFVCKVEDVNAVEAWIGSLPGQAYADLRRPLVSSL
                     NLCDMMPMSAIWSGPTINAHLSAECQKRGHPGPQPALLVTRTAGTTPFRFDLHQGDVG
                     HTMIVGPTGSGKSVLLNALAMQWLRYPEAQVFYFDKGASSRASTLLVGGQFYVLGGDQ
                     SELAFQPLAQIDAPDDRAWAQEWVQDIVAAEGVPITPPVKDEIWGGLRNLAEGPRDQR
                     TLT"
     assembly_gap    2179..2188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2189..2347)
                     /locus_tag="PLH31_10505"
     CDS             complement(<2189..2347)
                     /locus_tag="PLH31_10505"
                     /inference="COORDINATES: protein motif:HMM:NF010447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89669.1"
                     /translation="MLYLREYRPKADRLFDHLPWVALIGPGLVLNKDGSFQKTLAFRG
                     PDLASATDA"
BASE COUNT          412 a          724 c          879 g          403 t
ORIGIN      
        1 ggagctggct gatgagctgt tgatcgccgg gcgacgacga gcccacggcg gccagggaca
       61 cgccgcccaa gcccagctcg aacagccggt tcccggatgt ggactggtag tagtattcgc
      121 gcttaggcgt ggcgttggcg atgatccgga cctgctgcgg gttcaggccg aagtcctgat
      181 agagcgccgt gatctgcggt gtctgggcgt ccggattggg caagaagata cgggtcggac
      241 agctttcgat tagcgcaggc gcgatcgacg accgggcgac atccgccagg ctctgggtgg
      301 cgaacaccac ggcgacgttg aacttgcgca gcgtcttcag ccactcgcgg atcttggccg
      361 cgaaggcgcc ctggtcgagg aagagccaag cctcatcgag caccagcagc gtcggccgac
      421 cgtcgaagcg acgttcaagg ctgcggaaga tgtaggtgag gaccggggcc aaggccgact
      481 tgctggccat cagctcggcc atctcgaagg tctgccaggc ggcaggcttg agggcgttct
      541 ggttggcgtc gagcagatgg ccgtaaggtc cgctcagcgt gtagggcttc agggccgcct
      601 tgacggcatg gtcctggatg gtggcggcca gcnnnnnnnn nngggtgagg gtccgttgat
      661 cgcgcggccc ctcggcgaga ttgcgcagtc cgccccagat ctcgtccttt accggcggcg
      721 tgattggcac gccctcggcg gcgacgatgt cttgcaccca ttcctgggcc caggcgcggt
      781 cgtcgggcgc gtcgatttgc gccagcggct ggaaggccag ctcgctctgg tcgccgccca
      841 ggacatagaa ctggccaccg acaagcagcg tcgatgcgcg ggagctggcg cccttgtcga
      901 aatagaagac ctgagcttcc ggataacgca gccactgcat ggccagggca ttgagcagga
      961 ccgatttgcc cgagccggtc ggcccaacga tcatcgtgtg accgacatcg ccctggtgca
     1021 ggtcaaagcg gaagggcgtg gtgccggcag tgcgggtgac caacagagcc ggctgagggc
     1081 caggatggcc gcgcttctgg cattcggccg acaagtgggc gttgatcgtc gggccagacc
     1141 agatcgccga catcggcatc atgtcgcaga ggttcagcga cgacaccaag gggcgacgca
     1201 gatcggcata ggcttggccc ggcaggctgc cgatccaggc ctcgacagcg ttgacgtcct
     1261 cgaccttgca gacgaagccc gcccggttga tcgcgccttc gacctctctg acgcggttgg
     1321 ccaggcggtc tggatcggcg tccatcaggg tgacggtcgg ggtgaagtag ccgatcgagg
     1381 cgtagtcacc gccgagaatg gccagggccg catcggcgtc gacggacttc gccgccgcgt
     1441 ccggatcttc caggagggac ggctcacggg tgatcgcctc cttcagaagc gccatcacgc
     1501 ccttgcgttt ggcgaaccag cgcttgcgca ccgtggtcac ggccttgcgc gcgtcttcct
     1561 tgtccagcgg cagatagcgg caaacccagc gatagctgat gcccagctca ttgagccggt
     1621 cgagcaggcc ggggcacgag gtggtcgggt aggcccgaac cgagagggtg cgcaggtact
     1681 ggcccccgag gcggggcagc aggccgccct ggaaatcgtc gtcggtgagg aaggcgtcga
     1741 ggtaggccgg ggtctcaggt gtggcgaccg gatggcgctt ggtcgaaatg cagctgtgaa
     1801 gataggtcag ggtctcatcg tcggtcagtt ctgcgacctc gggcatgatc gccgtcagga
     1861 tgtcggcgat ctggtggacc gtactgagga agtcgctcag cgccgcgcgg tacatcccct
     1921 cggcgccgcg tcctgacggg gcgttttcaa gcaggaggct ctcggccgtg gagatcgcct
     1981 cttccggcgg caggtaggtg aaggtcagga agtaacggct ctcgaagtga cgctcctgcg
     2041 cctcgaagcc ctcgcggcgc tcctcatcga ccaggtccga gacggggtct ggaaactggc
     2101 ttgtcggata ggtctgcgat ggcgcgcgga cggcctcgat gtgcaggcac cagcgtgacc
     2161 ccaggcggcg caaggcatnn nnnnnnnncg cgtccgtggc gctggcgagg tccgggccgc
     2221 ggaaggccag ggtcttctga aagctgccgt ccttgttgag caccagcccg ggcccgatga
     2281 gggctaccca ggggaggtgg tcgaataggc gatcagcctt gggccggtac tcacgcaggt
     2341 acagcacagc gaacctctag gagaccaaaa gcggggaggg gtgaggccga gcgcgcatgt
     2401 caggcgtcca ggtagggctt ctggcgaatg tggcggcc
//
LOCUS       DAOMEO010000448         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4623_length_2434_cov_3.577133, whole genome shotgun sequence.
ACCESSION   DAOMEO010000448 DAOMEO010000000
VERSION     DAOMEO010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_2434_cov_3.577133"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..921
                     /locus_tag="PLH31_10510"
     CDS             136..921
                     /locus_tag="PLH31_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR89670.1"
                     /translation="MAQNTGRVAGKKAFITGGAQGLGAAAGRMLASQGAKVSLADINL
                     AGAQAVADEINAAHGAGTAFAFELDVTQEDEWIAVLEKATAAMGGLSVLVNNAGIGGD
                     GPIESLDFALWKKVMSVNVDSVFLGAKHALTHMRAHQPGSIINLSSIAGLIANGNSPA
                     YNASKAAVWLLSKNIALYCAKMKLDIRSNSIHPTFIDTPILDGFTARFGKEEAHAKLA
                     RQVPLGRIGEPNDIANAVLYLASDESKFMTGAEIKVDGGISAM"
     gene            complement(1101..1658)
                     /gene="spdR"
                     /locus_tag="PLH31_10515"
     CDS             complement(1101..1658)
                     /gene="spdR"
                     /locus_tag="PLH31_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase response regulator
                     transcription factor SpdR"
                     /protein_id="HQR89671.1"
                     /translation="MADISELVAALPDKTLLLLDDDAPLRTRLGRALEQRGFVVTLAA
                     SVAEALALLRAHAPAHAVLDMRLEDGTGLKVVEAVRDARPDAKIIMLTGYGNIATAVA
                     AVKAGVVDYLSKPADADDVARALLAAKDAAPAPPENPMSADRVRWEHIQRVYEMCGHN
                     VSETARRLNMHRRTLQRILAKRAPR"
     gene            complement(1735..>2434)
                     /locus_tag="PLH31_10520"
     CDS             complement(1735..>2434)
                     /locus_tag="PLH31_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR89672.1"
                     /translation="RLSALGALAAAAAHELGTPLATISVVAREMARNAPDDQTREDAE
                     LMIGQAARCREILQRLTEMPEAKDAVHERMSLVQLVQDVIEPHLVHGVRVEAVVNGPS
                     GESAPDIWRRPEVIHAMTSIVENAVDFARAEVLVIARFDPRSIVIEVRDDGPGFSAEV
                     LAKLGEPYVTTRPGAEGSRTGHIGMGLGFFIAKTLLERTGATVDFRNGRRGGAVVSAR
                     WPRSALEAPGYTGA"
BASE COUNT          402 a          839 c          810 g          383 t
ORIGIN      
        1 ggaggtggcg cggcgcggat acgcgacgtg acggaggggg cgtccggcgc tggccacctc
       61 accaaagccg tttccccctt ccgccccgcc cccatccagt tcaaactaat gtttgaataa
      121 tcgggaggac gccaaatggc gcagaacacg ggccgggtcg ccggcaagaa ggccttcatc
      181 acgggcgggg ctcagggtct gggcgcggcg gcggggcgga tgctggccag tcagggcgcc
      241 aaggtcagtc tggccgacat caatctggcg ggcgctcaag ccgtggccga tgagatcaac
      301 gccgcccacg gggccggcac ggccttcgcc ttcgaactgg atgtcaccca ggaagatgag
      361 tggatcgcgg ttctggagaa ggcgacagcg gccatgggcg gcctgtcggt gctggtcaat
      421 aatgccggca tcggcggcga cggccctatc gagagcctgg atttcgccct ctggaagaag
      481 gtgatgtcgg tcaatgtcga ctcggtgttc ctcggcgcca agcacgccct gacccacatg
      541 cgggcccatc agcccggctc gatcatcaat ctgagctcga tcgccggcct gatcgccaac
      601 ggcaattcgc cggcctacaa cgcctccaag gcggcggtct ggctgctgag caagaacatc
      661 gcgctctact gcgccaagat gaaactggat atccgctcca actcgatcca ccccaccttc
      721 atcgacacgc ccatcctgga cggcttcacg gcgcggttcg gcaaggaaga ggcccacgcc
      781 aagctggcgc ggcaggtgcc cctgggccgc atcggcgagc ccaacgacat cgccaacgcc
      841 gtgctctatc tggccagcga cgagagcaag ttcatgaccg gcgccgagat caaggtcgac
      901 ggcggcattt cggcgatgtg atgaaaatcc cttctcccct tgcgggagaa ggtgtcgccg
      961 aaggcgacgg atgaggggtt gaaaaacgca agacgccgcg atcggccttt cggcttgtcg
     1021 cggcgtttct gattgcgcgc cccctcaccc gacccgcttc gcgggccacc ctctcccgca
     1081 aggggagagg ggaaccggct tcagcgcggc gcccttttcg ccaggatccg ctgcagagtc
     1141 cgtcggtgca tgttcagccg ccgcgcggtc tcagacacgt tgtggccgca catctcatag
     1201 acccgctgga tatgctccca tcgtacgcgg tcggcgctca tcgggttttc cgggggcgcc
     1261 ggggcggcgt ccttggccgc cagcagggcg cgcgccacgt cgtctgcgtc ggccggcttg
     1321 gagagatagt ccaccacccc ggccttcacg gcggccacgg cggtggcgat gttgccatag
     1381 ccggtgagca tgatgatctt ggcgtcgggt cgggcgtcgc ggaccgcctc gactaccttc
     1441 agccccgtgc cgtcttccag ccgcatgtcg agcacggcgt gggccggggc gtgagcccgc
     1501 agcagggcca gggcctcggc caccgaagcg gccagggtca ccacgaaccc gcgctgctcc
     1561 aaggctcgcc ccagccgcgt gcgcaggggc gcatcgtcgt cgagtagcag cagcgtcttg
     1621 tcgggcaggg ccgcaaccag ctcactaata tccgccatga attcagcgcc tccccgcgct
     1681 tacactcaac cgagtgtact catttgacat catgtcgccc ttctcggtcc cgcatcaagc
     1741 ccccgtgtag ccaggggctt caagcgccga ccggggccaa cgggccgaga ccaccgcccc
     1801 gccgcgtcgg ccattgcgga aatccaccgt ggcgccggtg cgctccagca gggtcttggc
     1861 gataaagaag cccaggccca tgccgatatg gcccgttcgc gagccctcgg cgcccgggcg
     1921 cgtggtgaca tagggctcgc ccagcttggc cagcacctcg gccgagaagc cgggcccatc
     1981 gtcgcgcacc tcgatgacga tcgagcgcgg atcgaagcgg gcgatgacca gcacctcggc
     2041 gcgggcgaaa tcgaccgcgt tctcgacgat cgaggtcatg gcgtggatca cctcgggccg
     2101 gcgccagata tccggggcgc tctcgccgga cggaccattg accacggcct cgacgcgcac
     2161 gccgtgcacc agatgcggct cgatgacgtc ctggaccagc tggaccaggc tcatgcgctc
     2221 gtgcaccgcg tccttggcct cgggcatttc ggtcaggcgc tgcaggatct cgcgacagcg
     2281 ggcggcctgg ccgatcatca gctcggcgtc ctcgcgggtc tggtcgtcag gcgcgttgcg
     2341 agccatttcg cgggccacta ccgagatggt cgccagcggc gtacccagct cgtgggcggc
     2401 tgcggcggcc agggcgccca gggccgacag gcgc
//
LOCUS       DAOMEO010000449         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4625_length_2434_cov_2.480454, whole genome shotgun sequence.
ACCESSION   DAOMEO010000449 DAOMEO010000000
VERSION     DAOMEO010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_2434_cov_2.480454"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="PLH31_10525"
     CDS             complement(<1..2166)
                     /locus_tag="PLH31_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQR89673.1"
                     /translation="MFSESFMARAARRTAVELFWDAIRYGWAQPWSARFRGGLVTVVG
                     AGLLLAVATYNYDDPSLNAATGAEATNALGGAGAAIADILMQSLGLVGWLAALLMLVF
                     GLTRVSQADPAAERHDLRLRALVGVAGLLALAGVLAALPTPAIWQSAKGLGGFWGDGL
                     LHVIAAVFDFARLPGAELIAAILLAIGAAIALGFAIGFRRFDPEAIAHAVNGALQPRE
                     RAEPEPVAPAPKAPRARKVVETPVPPRRPARPEAEVPEQAYTPPPVAADDEDEDQPVA
                     ARPLAIAKPKTLPKDSGREQREQQKAFNFNDEGGFQLPELAMLAKAKPRSSDVDAAAL
                     RQNARLLESVLAEFGVKGQIDQIRPGPVVTMYELVPAPGVKTARVVALADDIARSMSV
                     ISCRVAVAQGRNAIGIEMPNSRRETVYLRDLLSSNDYDKASSILPMALGETIGGEPYI
                     ADLAKMPHLLIAGTTGSGKSVGVNAMILSILYKLPPEKCRFIMIDPKMLELSVYDGIP
                     HLLAPVVTDPRKAIVALKWTVREMEDRYRRMSKIGVRNIGGFNDKANEALEKGEHFER
                     TVQTGFDDAGRPIYETEQIRPEAMPYLVVVIDEVADLMMVAGKDIEGAVQRLAQMARA
                     AGIHLIMATQRPSVDVITGTIKANFPTRISFQVTSKIDARTILGEQGAEQLLGQGDML
                     YMAGGGRVTRLHGPFVSDGEVEEVAKFLRDQGTPNYLEEV"
BASE COUNT          371 a          762 c          893 g          408 t
ORIGIN      
        1 gacctcttcc agatagttgg gcgtgccctg gtcgcgcagg aacttggcca cctcctcgac
       61 ctcgccgtcg ctgacgaacg ggccgtgcag gcgggtgacc cgcccgccgc cggccatgta
      121 gagcatgtcg ccctggccca gcagctgctc ggcgccctgc tcgcccagga tggtgcgggc
      181 gtcgatcttt gaggtgacct ggaagctgat ccgggtgggg aagttggcct tgatggtgcc
      241 ggtgatcacg tcgaccgacg ggcgctgggt ggccatgatc aggtggatgc cggcggcgcg
      301 ggccatctgg gccaggcgct ggacggcgcc ttcgatgtcc ttgccggcca ccatcatcag
      361 gtcggcgacc tcgtcgatga cgacgaccag atagggcatg gcttcggggc ggatctgctc
      421 ggtctcgtag atcgggcggc cggcgtcatc gaagccggtc tggacggtgc gctcgaaatg
      481 ctcgcccttt tccagggctt cgttggcctt gtcgttgaag ccgccgatgt tgcgcacgcc
      541 gatcttggac atccgccgat agcggtcctc catttcgcgc acggtccatt tcagggcgac
      601 gatggccttc ctgggatcgg tcacgaccgg ggccagcagg tgcgggatgc cgtcatagac
      661 cgacagttcc agcatcttgg ggtcgatcat gatgaagcgg cacttctcgg gcggcagctt
      721 gtacaggatc gacaggatca tggcgttgac gcccaccgac ttgccagagc cggtggtgcc
      781 ggcgatcagc aggtggggca tcttggcgag atcggcgata tagggctcgc cgccgatggt
      841 ttcgcccagc gccatgggca ggatcgagct ggccttgtca tagtcgttgc tggacagcag
      901 atcgcggaga tagacggtct cgcgccgcga attgggcatt tcgatgccga tggcgttgcg
      961 gccctgggcc acggcgacgc ggcaggagat cacgctcatc gagcgggcga tgtcatcggc
     1021 cagggccacg acgcgggcgg tcttgacgcc gggggccggg accagctcgt acatggtgac
     1081 tacggggccg gggcggatct ggtcgatctg gcccttgacg ccgaactcgg ccagcacgct
     1141 ttccagcagg cgggcgttct ggcgcagggc ggcggcgtcg acgtccgacg agcgcggctt
     1201 ggccttggcc agcatggcca gctcgggcag ctggaagccg ccttcatcgt tgaagttgaa
     1261 ggccttttgc tgctctcgct gctcgcggcc tgagtccttg ggcagggtct tgggcttggc
     1321 gatggccagg gggcgggccg cgacgggctg gtcctcgtcc tcgtcatcag cggcgacggg
     1381 cgggggcgta taggcctgtt ccgggacctc ggcttcgggc cgggccgggc ggcgcggcgg
     1441 gaccggggtc tcgacaacct tgcgggcgcg cggcgccttg ggcgcggggg cgacaggttc
     1501 gggctcggcc cgctcgcgcg gttgcagggc gccgttcacg gcgtgggcga tggcttccgg
     1561 atcaaagcgg cggaagccga tggcgaagcc cagggcgatg gcggcgccga tggccagcag
     1621 gatggcggcg atcagctcgg cgcccggcag gcgggcaaag tcgaagacgg cggcgatgac
     1681 gtgcagcagg ccatcgcccc agaacccgcc cagccccttg gcggactgcc agatagcggg
     1741 ggtcggcagt gcggccagca cgccggccag ggccagcagg ccggccacgc ccaccagggc
     1801 gcgcaggcgc aggtcgtgac gctcggcggc gggatcggcc tgcgacaccc gggtcaggcc
     1861 gaagaccagc atcagcaggg cggccagcca gcccaccagg cccagcgact gcatcaggat
     1921 gtcggcgatg gccgccccgg ctccgccaag ggcgttggtg gcttccgcgc cagtggcggc
     1981 gttcaggctg gggtcgtcgt aattataggt ggcgacggcc agcagcaggc cggcgccgac
     2041 cacggtgacc agcccgcccc ggaaccgcgc cgaccacggc tgggcccagc catagcggat
     2101 ggcatcccag aacagctcca ccgccgtacg ccgcgccgct cgcgccatga aactctccga
     2161 aaacaccggg taccgaactc tcgaaccgcc atgttgtgcc tgaagagggt taaggggcgt
     2221 ttacgctgat cgtcatcagg gtgattcagt gggtcttggg cttgcctgac cagcttcaca
     2281 ggacccggag cgttgagatg tagcgcatga cgctacattt gcttgctttc cgtagtgtct
     2341 cgcgctacat ctccgagcga gaggcgcgag atgatccaaa gctggaaagg caagtttgcc
     2401 gaagaggtct ttctggggcg ttcgcccaaa ggtt
//
LOCUS       DAOMEO010000450         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4631_length_2432_cov_2.424064, whole genome shotgun sequence.
ACCESSION   DAOMEO010000450 DAOMEO010000000
VERSION     DAOMEO010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4631_length_2432_cov_2.424064"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..839
                     /locus_tag="PLH31_10530"
     CDS             51..839
                     /locus_tag="PLH31_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89674.1"
                     /translation="MRRSATALLLILSLAATGPAMAGETACWFENGAIVAPAAIADMA
                     GDFVIDLSAPRTLLHNTKAQMSGYAETDLTLPVRLAGQRVAAAPIRVTDLDYRGVGFV
                     APIAGVIGADILDRYVVVIDFAPCRLQLEVRDAVRAPAGRALPVTVVGGVPTVRATAS
                     DGFTSLSGAFALDTASNGGVRARGDADGPRQTPAGTLAALSLDGRLSQRVPAVRAGDL
                     PEGVAGAVGVAILSDYHLRLDTLARRLWLTPAAKEKGPGVSARP"
     gene            complement(863..1707)
                     /locus_tag="PLH31_10535"
                     /pseudo
     CDS             complement(863..1707)
                     /locus_tag="PLH31_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
     gene            complement(1713..>2432)
                     /locus_tag="PLH31_10540"
     CDS             complement(1713..>2432)
                     /locus_tag="PLH31_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HQR89675.1"
                     /translation="PFMIAGVVILHIWALHVVGQNNPTGVEPKSKADTLPFTPYATVK
                     DGFAMSVFLILFAFFVFYMPNALGHADNYIPANPLVTPAHIVPEWYFLPFYAILRAVP
                     DKLMGVLAMFGAIACLFALPWLDTSKVRSMRYRPTAKMYFFIFVVACCILGLCGAKLP
                     DDPVIPHVKTFLLIDADLNSFVWLSRAATLYYFGFFLVILPILGLKETPLPVPESIAS
                     PALSHPAGLPAHATAAPEMKG"
BASE COUNT          403 a          784 c          858 g          387 t
ORIGIN      
        1 ccgtgctgac gcctgtcgcc gggaccgaaa ggcttggcta ggatcaacgc atgcgccgct
       61 ccgccaccgc cctgctgttg atcctgtcgc tggcggcgac cggcccggcc atggccggcg
      121 aaacggcctg ctggttcgag aacggggcca tcgtcgcccc ggccgccatc gccgacatgg
      181 ccggcgactt cgtcatcgat ctgtcggcgc cgcgcaccct gctgcacaac accaaggccc
      241 agatgagcgg ctatgccgaa accgacctga ccctgccggt gcgactggcc ggacagaggg
      301 ttgcggccgc cccgatccgg gtcaccgatc tggactatcg cggcgtgggc tttgtcgcgc
      361 cgatcgccgg ggtgatcggg gccgatattc tggatcgcta tgtggtggtc atcgacttcg
      421 ccccctgccg gctgcagctg gaggtgcggg acgcggtgcg cgcgcccgcc gggcgggccc
      481 tgcccgtaac ggtcgtcggc ggcgtgccga ccgttcgggc cacggcatcc gacggcttca
      541 ccagcctgag cggggccttc gccctcgata ccgcctccaa cggcggggtc cgggcgcgcg
      601 gcgacgccga tgggccgcgc cagaccccgg cggggaccct ggccgcgctg tcgctggacg
      661 gtcgcctgag tcagcgcgtt cccgctgtgc gagccgggga cctgccagaa ggcgtggcgg
      721 gcgcagtggg cgtggccatc ctgtcggact accacctgcg cctcgacacc ttggccaggc
      781 gtctctggct gacccccgcc gccaaagaaa aagggcccgg cgtttccgcc aggccctgat
      841 cgcttcagtc gtttccgacg ggtcagtgcg actcgttctt ccagaccttg cggtagctgg
      901 catagagcag ccccgagaac agcagcagat acaggatcac cacgaagccg gtttccttgc
      961 gctcttccat ctgggctcgg cggcccacat caggaaggcc gagacgtcct tggcctgctg
     1021 gtcgatggtg gacggggtgc cgtcgtcaaa cgacaccagg tccttcttga gcggcgcggc
     1081 catggcgatg tagccgccgg gcgggacctt gtcatgcgga cccgaccacg aggcggtaag
     1141 atcaccggcc atataggggt tgtagtgctg gccggcgccg accttcaggc cggcgggcgg
     1201 ggccacatag ccggtcagca gcgagtacag atagtccggg ccgccggtgc gggccttggc
     1261 cagcagcgac atgtccggcg gcagggcgcc gccattaccg gcccgggcgg ccgcctcgtt
     1321 gggatagggc gaggtgaaac gatccgccga ggtcgccgga cgcttgatcg cgtcgccggt
     1381 ctcggagtcg atatcggcga cctcgtagtc cttggcgatc gccttgaccc acgggttgtc
     1441 gttcgggttc ttgtactcgg cgttatagaa cggaccgccc ttgtcgccga ggttgcggaa
     1501 gctgaccagg tgcatcgagt ggcaggccga gcagacctcc cgatagacct tgtagccgcg
     1561 ttgcagctgg gcctggtcga acttgccgaa cgggccttca aacgaccatt cgacattctt
     1621 gggcggcagg gggtgacctg ccgccagaac cggaccagcc gccatcaggc cggccacggc
     1681 tgcgaggata gagagtttgc gcagcatcat gatcagccct tcatctcagg cgcggcggtc
     1741 gcatgggcag gcaggcctgc ggggtgcgac agggccggcg aagcaatcga ttccggcacg
     1801 gggagcggcg tctccttgag gcccaggatc ggcaggatca ccaggaagaa gccgaagtag
     1861 tacagcgtgg ccgcccggct gagccagacg aagctgttca ggtcggcgtc gatcagcagg
     1921 aaggtcttca cgtgcgggat gaccggatca tccggcagct tggcgccgca caggcccagg
     1981 atgcagcacg ccacgacgaa gatgaagaag tacatcttcg cggtcgggcg gtagcgcatc
     2041 gagcgcacct tcgaggtgtc cagccatggc agggcgaaca ggcaggcgat ggcgccgaac
     2101 atggccagca cgcccatcag cttgtcggga acggcgcgca ggatcgcgta gaacggcagg
     2161 aagtaccatt ccggcacgat gtgcgcgggc gtcaccagcg ggttggccgg gatgtagttg
     2221 tcggcgtggc ccagggcgtt gggcatgtag aacacgaaga aggcgaacag gatcaggaac
     2281 acgctcatcg caaacccgtc cttcaccgtc gcatagggcg tgaagggcag ggtatcggcc
     2341 ttcgacttgg gctcgacgcc ggtcgggttg ttctggccga ccacgtgcag ggcccagatg
     2401 tgcaggatca cgacgcccgc gatcatgaag gg
//
LOCUS       DAOMEO010000451         2431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4637_length_2431_cov_2.495370, whole genome shotgun sequence.
ACCESSION   DAOMEO010000451 DAOMEO010000000
VERSION     DAOMEO010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_2431_cov_2.495370"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..2219)
                     /gene="parE"
                     /locus_tag="PLH31_10545"
     CDS             complement(126..2219)
                     /gene="parE"
                     /locus_tag="PLH31_10545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQR89676.1"
                     /translation="MSSKDDPNSLFSLFDDALAPTPAAPLPAREPHVEAPARAAAPPP
                     PSAAPAPASSGGYTASDIEVLEGLEPVRKRPGMYIGGTDERALHHLFAEVLDNSMDEA
                     VAGFAKTIEVKLDADGFLSVKDDGRGMPVDPHPKHPGKSALEVIMTVLHAGGKFSGKA
                     YETSGGLHGVGASVVNALSERVEVTVWRDGFEHLQVFSRGKPLGPVEKIGPSRKRGTM
                     VRFKPDDEIFGEGTNFKPARLYRMARSKAYLFRGVQIKWSCAAERIHDQTPAEATFHF
                     PNGLADFLAERTKGLETVTPESFAGRIERQGEAGAVEWAIAWTPRGFGEHDGFMQSYC
                     NTVPTPEGGTHESGLRAALTRGLKAYAELKGEKRAAIITADDVIAQAGALISVFIRNP
                     EFQGQTKEKLSSSEAQRFVEAALRDPFDHWLTASPKNAQALLDFVIERAEERLKRRKD
                     KEVSRASATRKLRLPGKLADCAGGAVDGAELFIVEGDSAGGSAKQARNRKNQAILPLR
                     GKILNVASATGEKMSANKELSDLMLALGAQGGAKYREEDLRYERIIIMTDADVDGAHI
                     ASLLITFFYRTMPELIRGGHLFLALPPLYRISHGGKSAYARDDAHKDELLATQFKGKK
                     PEIGRFKGLGEMMAAQLKETTMDPAVRTLARVTLPRSEEAVESLVETLMGRKPELRFR
                     FIQENAEFAAADLDV"
BASE COUNT          345 a          776 c          882 g          428 t
ORIGIN      
        1 gatttcctgg cgatcatcgc ggcggcctag aggctcgcgg tggcccgggg accgggatcg
       61 cacagtctgc agctgggccg acgattgagg cggcgtcccg ctcctccctc gcggggaagt
      121 acgtcctaga cgtccagatc cgccgccgcg aactcggcgt tttcctggat gaaccggaac
      181 cgcagttcgg gcttgcgacc catcagggtc tcgaccagcg actccaccgc ctcctcggag
      241 cggggcagag tcacccgcgc cagggtgcgg acggccggat ccatggtcgt ctccttgagc
      301 tgggcggcca tcatctcgcc caggcccttg aagcggccga tctcgggctt cttgcccttg
      361 aactgcgtgg ccagcagttc gtccttgtgg gcgtcgtcgc gggcataggc gctcttgccg
      421 ccgtggctga tgcggtacag cggcggcagg gccaggaaca gatgcccgcc ccggatcagc
      481 tcgggcatgg tgcggtagaa gaaggtgatc agcagcgagg cgatgtgggc gccgtcgaca
      541 tcggcgtcgg tcatgatgat gatccgctcg tagcgcagat cttcctcgcg atacttggcg
      601 ccgccctggg ctcccagggc cagcatcagg tccgacaact ccttgttggc gctcatcttt
      661 tcgccggtgg ccgaggcgac gttgaggatc ttgccccgca ggggcaggat cgcctggttc
      721 ttgcggttgc gggcctgttt ggccgagccg ccggcgctgt caccttcgac gatgaacagc
      781 tcggcgccgt cgaccgcgcc gccggcgcag tcggccagct tgcccggcag gcgcagtttg
      841 cgggtcgccg aggcgcggga gacttccttg tccttgcggc gcttgaggcg ttcctcggcg
      901 cgctcgatga cgaagtcgag cagggcctgg gcgttcttcg gcgaggcggt gagccagtgg
      961 tcgaacgggt cgcgcagcgc cgcttcgacg aagcgctggg cctcgctcga ggacagcttt
     1021 tccttggtct ggccctggaa ttcgggattg cggatgaaca ccgagatcag ggccccggcc
     1081 tgggcgatga cgtcgtcggc ggtgatgatc gccgcccgct tctcgccctt cagttcagca
     1141 taggccttga ggccccgggt cagggcggcg cgcaggccgc tctcgtgggt gccgccttcg
     1201 ggagtgggca cggtgttgca gtacgactgc atgaagccgt cgtgctcgcc gaaaccgcgc
     1261 ggggtccagg cgatggccca ttcgaccgcg ccggcctcgc cctggcgctc gatgcgaccg
     1321 gcgaagcttt caggcgtgac ggtttccagg cccttggtgc gctcggccag gaagtcggcc
     1381 aggccgttgg ggaagtggaa tgtggcctcg gcgggcgtct ggtcgtggat gcgctcggcg
     1441 gcgcaggacc atttgatctg cacgccgcgg aacagatagg ccttggagcg ggccatgcgg
     1501 tacaggcggg cgggcttgaa attggtgccc tcgccgaaga tctcgtcgtc gggcttgaag
     1561 cgcaccatgg tgccgcgctt cctgctgggg ccgatcttct cgaccggacc caggggcttg
     1621 ccgcgcgaga acacctgcag gtgctcgaac ccgtcgcgcc agacggtgac ctcgacccgc
     1681 tccgacaggg cgttgacgac gctggcgccg acgccgtgca ggccgccgga ggtctcatag
     1741 gccttgccgc tgaacttacc gccggcgtgc aggacggtca tgatcacctc cagcgccgac
     1801 ttgccggggt gcttgggatg ggggtcgacc ggcatgcccc ggccgtcgtc cttgaccgac
     1861 aggaagccgt cggcgtcgag cttgacctcg atggtcttgg cgaagccggc cacggcctcg
     1921 tccatcgagt tatccagcac ctcggcgaac agatggtgca gggcccgctc gtcggtgccg
     1981 ccgatgtaca tgcccggccg cttgcggacg ggctccagac cttccagcac ctcgatgtcg
     2041 ctggccgtgt agccgcccga cgaagccggt gcaggcgcag cggatggagg cggtggcgcg
     2101 gcggcgcggg ccggggcctc gacgtgcggc tcgcgagccg gcaatggcgc ggccggcgtc
     2161 ggcgccaggg cgtcgtcgaa caacgagaac agcgagttgg gatcgtcttt agaggacatt
     2221 acgggaacat gcgtcgattc gatggaagct tcgtatgggc ttcccggagt ccggtgaaaa
     2281 gaggcgtgtc gtcgcggggg gcggatttgt tggggagcaa gatgctcccc tcggtggggg
     2341 aggaggcccg tagggccgga gggggaagac cgcaatgcgg cccagaactt ccccctccgt
     2401 cgcctccggc gacacctcca tagctgattc t
//
LOCUS       DAOMEO010000452         2430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4640_length_2430_cov_3.330947, whole genome shotgun sequence.
ACCESSION   DAOMEO010000452 DAOMEO010000000
VERSION     DAOMEO010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_2430_cov_3.330947"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..495
                     /locus_tag="PLH31_10550"
     CDS             103..495
                     /locus_tag="PLH31_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HQR89677.1"
                     /translation="MPAAFDDIEIGQVVSLGTRAVEARALDDFIAAFAPGWSAENGAP
                     DVMVFAIWSRLDAEAAAAWPQTKRLGVDALRWMRNPPVGELLRGRMTVMGKDPVGEGK
                     GMIIAQHDLLDEAGRLVFSCLTRSIFAR"
     gene            531..1157
                     /locus_tag="PLH31_10555"
     CDS             531..1157
                     /locus_tag="PLH31_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR89678.1"
                     /translation="MITVWGGQTSRSIRVVWVLEEMGLPYRVRQVDMLAAEQDPEFLA
                     VNPADYIPAIQDGDVVMVESIAIMEYLMARYGPTPLAPAPQDPTFPAYQQFLHLGEAG
                     LATLMMPVIVSRFIAPEAERANWGATWCLKSFQKRLKLVSQRLSSSPYLAGEAFTAAD
                     ISVTYALNLGQRTCGITLGDAEQAYLARTTARDAYKRAMERSHEGVDT"
     gene            complement(1308..2099)
                     /locus_tag="PLH31_10560"
     CDS             complement(1308..2099)
                     /locus_tag="PLH31_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HQR89679.1"
                     /translation="MESTQSAGGISGNVLFYASPEPLNREQHAKLALVHNEKPYSFAA
                     AGTAVPLTVTEFAPAALSFPVIFAGEDRVPLAVMGLNNGENLFVNADGSIDPGVYIPA
                     YIRRYPFVLANDEGQDRLIVCIDKGSSLLSETGETKLFDEKGEPTEYTQNCIKFCDDF
                     EAERRRTDSFVQLLKDNDLFELKTAIFTPTDAAGNAGPPQTVAEYYGVSEEKLNALPV
                     DKLRELQTNGALAQIYAHLVSLVGWDRLIALAMVRQAAAAGVAVN"
     gene            complement(2244..>2430)
                     /locus_tag="PLH31_10565"
     CDS             complement(2244..>2430)
                     /locus_tag="PLH31_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89680.1"
                     /translation="TDAYTTLNASLAWKPKGNGQLRVFLDGRNLTDEEVREHASFLKD
                     IAPSAGRSIRAGVAYRF"
BASE COUNT          424 a          800 c          803 g          403 t
ORIGIN      
        1 ctcgcacaaa acctgcgccc gacacatggg ccggccgagc gctctcgaca agcgccgagc
       61 gagcatggac agtcaaggac gactcaccgt gagacctccg acatgcctgc cgcctttgac
      121 gatatcgaga ttggtcaggt cgtgtccctg ggcacgcgcg cggtcgaggc cagggcgctg
      181 gacgacttca tcgcggcgtt cgccccaggc tggtccgccg agaacggcgc ccccgacgtg
      241 atggtcttcg cgatctggtc gcggctcgac gccgaggccg ccgccgcctg gccgcagacc
      301 aagcgcctgg gcgtcgacgc cctgcgctgg atgcgcaatc cgccggtcgg cgaactgctg
      361 cgcggccgca tgacggtgat gggcaaggac ccggtgggcg aaggcaaggg catgatcatc
      421 gcccagcacg acctgctcga cgaggcggga cggctggtgt tttcctgcct gacgcggagc
      481 atcttcgcgc ggtagctcga ctttcagtca cgccaggaaa gggccagcgc atgatcaccg
      541 tttggggcgg acagacctcg cgatcgatcc gggtcgtctg ggtgctggag gagatgggtc
      601 ttccctaccg cgtgcggcag gtggacatgc tggccgccga gcaggacccc gagttcctgg
      661 cggtgaaccc cgcggactac atcccggcca tccaggacgg cgacgtcgtc atggtcgagt
      721 cgatcgcgat catggagtac ctgatggccc gctacgggcc gacgccgctg gcgcccgcgc
      781 cgcaggatcc gaccttcccc gcctaccagc agtttctgca cctgggcgaa gccggactcg
      841 cgaccctgat gatgcccgtg atcgtcagcc gcttcatcgc gcccgaggcc gagcgggcca
      901 actggggcgc gacgtggtgt cttaagtcgt tccagaagcg gctgaagctg gtcagccaac
      961 ggctttccag ctcgccctac ctcgctggcg aggccttcac ggccgccgac atctcggtga
     1021 cctatgccct gaacctgggc caacggacct gcggcatcac cctcggcgac gccgaacagg
     1081 cctaccttgc ccgcaccacc gctcgcgacg cctacaagcg cgccatggag cgttcgcacg
     1141 agggcgtgga cacctaaacc ccgcctctgc cttgaatcga gaccggagag ggcagccctc
     1201 aacatccggg atgggtccgg aaacgcaact cacccccagc ccaagaaaaa aggcggcgaa
     1261 gccaaagcct cgccgccctt attcaaacct cccgaaggaa gcctgtctta gttaacagcc
     1321 acgcccgctg cggccgcctg gcggaccatg gccagggcga tcaggcgatc ccagccgacc
     1381 agcgacacca ggtgcgcata gatctgggcc agggcgccgt tggtttgcag ttcgcgcagc
     1441 ttgtcgaccg gcagggcgtt cagcttctct tccgatacac cgtaatattc ggccaccgtc
     1501 tgcggcgggc cggcgttgcc ggcggcgtcg gtcggggtga agatggcggt cttcagctcg
     1561 aacaggtcat tgtccttcag cagctgcacg aacgagtcgg tgcggcggcg ttcggcttcg
     1621 aagtcgtcgc agaacttgat gcagttctgt gtgtattcgg tgggctcgcc cttttcgtcg
     1681 aacagcttgg tttcgccggt ttccgacagc aggctcgagc ccttgtcgat gcagacgatc
     1741 aggcggtcct ggccttcgtc attggccagc acgaagggat agcggcggat ataggcgggg
     1801 atatagacgc ccggatcgat cgaaccgtcg gcgttgacga acaggttctc gccgttgttc
     1861 aggcccatca cggccagggg cacgcggtct tcaccggcga agatcaccgg gaacgacagg
     1921 gcggcgggcg caaactcggt gaccgtcagc ggcacggccg tgccggcggc ggcgaagctg
     1981 tagggcttct cgttgtgcac gagggccagc ttggcgtgtt gctcgcggtt cagcggctca
     2041 ggcgacgcgt agaacagaac attgccggag atgccgccgg cggactgggt cgattccatt
     2101 gatagctcta caaaaaagct gaaagggcgg tccgcgcgag cggccgtaaa gggcttggcg
     2161 cttctagccg atcccggcca ccccggcaat cgacccgtcc cgagcgggga tataaccccc
     2221 gccctttcca ctcaggagag accctagaac cggtaggcga cccccgcgcg gatcgagcgg
     2281 cccgccgaag gggcgatgtc cttcaggaac gaggcatgct cgcgcacctc ctcgtcagtg
     2341 aggttgcggc catcgaggaa gacccgcagc tggccgttgc ctttgggctt ccaggccaac
     2401 gaggcgttca gcgtcgtata ggcgtcggtg
//
LOCUS       DAOMEO010000453         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4647_length_2426_cov_2.592577, whole genome shotgun sequence.
ACCESSION   DAOMEO010000453 DAOMEO010000000
VERSION     DAOMEO010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4647_length_2426_cov_2.592577"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLH31_10570"
     CDS             <1..498
                     /locus_tag="PLH31_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HQR89681.1"
                     /translation="AALGNDVNLRDFEGRSALLLSKAKDNNASCAIGPFFRLFDDGFG
                     LDDVRSAEVELKITGRDNFVLDGRSNMSLISRDPAALAGQAFGKQHQYPDGFVLFLGT
                     MFAPIQDRDTPGQGFTHKVGDRVRVSTPRLGVLENEVTTCDQARPWTFGISALIRNLA
                     GRGLL"
     gene            521..>1338
                     /locus_tag="PLH31_10575"
     CDS             521..>1338
                     /locus_tag="PLH31_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR89682.1"
                     /translation="MTDTLRHYIGGERVASDAPTQSLNPSNTNDVVANVPNGGSAEVD
                     AAVAAAKKAFPSWSESSPEVRSDLLDKVGSTIIARSADIGRLLAREEGKTLAEGIGET
                     VRAGRIFKYFAGEALRRHGQNLESTRPGVEIQTYRQAVGVYGLVTPWNFPIAIPAWKA
                     APALAFGNTVVIKPAGPTPATASVLADIMQECGAPAGVFNMLFGRGSMGDALVKHKDV
                     DGVSFTGSQGVGAQVAAAAVARQARVQLEMGGKNPLIVLDDADLERAVAIALDGS"
     assembly_gap    1339..1348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1349..1929
                     /locus_tag="PLH31_10580"
     CDS             <1349..1929
                     /locus_tag="PLH31_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR89683.1"
                     /translation="LIVTDGIHDRFVALLAEKIAALRVGDALDANTQIGPAVSEDQME
                     TSYKYIDIATGEGGRVVTGGQRLQLENPGWYVQPTLIADTDRNMRINNEEVFGPVAST
                     IRVKDYEEALDVANGVEFGLSAGIATTSLKYARHFQRHAKAGMTMVNLATAGVDYHVP
                     FGGSKSSSYGAREQGFAAVEFFTQTKTSYSWS"
     gene            1935..>2426
                     /locus_tag="PLH31_10585"
     CDS             1935..>2426
                     /locus_tag="PLH31_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89684.1"
                     /translation="MSSAIYPSLKGKRVVITGGGSGIGAGLTTGFVRQGAEVIFLDIA
                     DEDSHALVAQLSTDAAIAPVYRHCDLMDLEGLKATLAEIGDVDVLVNNAGNDDRHKLA
                     DITPAYWDNRIGVNLRHMLFAAQAVAPGMAKRGGGAIINFGSISWHLGLEDLVLYETA
                     KAGI"
BASE COUNT          417 a          866 c          762 g          371 t
ORIGIN      
        1 gcggcgctgg gcaacgacgt caatctgcgc gacttcgagg gtcgctcggc cctgctgctc
       61 agcaaggcca aggacaacaa cgcctcgtgc gccattggcc ctttcttccg cctgttcgac
      121 gatggctttg gcctggacga cgtgcgctcg gccgaggtcg agctgaagat caccggccgc
      181 gacaatttcg tgttggatgg ccgcagcaac atgagcctga tcagccgcga ccccgccgcc
      241 ctcgcgggcc aggcgttcgg caagcagcat cagtatccgg atggctttgt cctgttcctg
      301 ggcaccatgt tcgccccgat ccaggatcgc gacacgccag gccagggctt tacccacaag
      361 gtcggcgacc gcgtccgcgt ctctaccccc aggctgggcg tgctcgagaa cgaggtcacc
      421 acctgcgatc aggccaggcc ctggacgttc gggatttccg ccctgatccg caatctggcc
      481 ggccgcggcc tcctctaaac ccaaatccta ggagctcggc atgaccgaca ccctgcgcca
      541 ctatatcggc ggcgaacgcg tcgcttccga cgccccgacc cagagcctga acccgtcgaa
      601 caccaacgac gtcgtagcca acgtgcccaa cggcggctcg gccgaggtcg acgccgctgt
      661 cgccgcggcg aagaaggcct tcccgtcgtg gtcggaaagc tcgccggaag tccgctcgga
      721 cctgctggac aaggtgggct cgaccatcat cgcccgcagc gccgacatcg gccgcctgct
      781 cgcccgcgaa gagggcaaga ccctggccga gggcattggc gagaccgtcc gcgccggccg
      841 catcttcaag tacttcgccg gcgaggccct gcgccgccat ggccagaacc tggaaagcac
      901 ccgtccgggc gtcgagatcc agacctatcg ccaggccgtc ggcgtgtatg gcctggtcac
      961 gccctggaac ttcccgatcg ccatcccggc ctggaaggcg gctccggctc tcgccttcgg
     1021 caacaccgtg gtgatcaagc ccgctggccc gaccccggcc accgcctcgg tcctggcgga
     1081 catcatgcag gaatgcggcg cgccggccgg cgtgttcaac atgctgtttg gccgcggttc
     1141 gatgggcgac gccctggtca agcacaagga cgtcgacggc gtgtcgttca ccggctcgca
     1201 gggcgtgggc gcccaggtcg ccgccgccgc cgtggcccgc caggcccgcg tgcagctgga
     1261 gatgggcggc aagaacccgc tgatcgtgct ggacgacgcc gatctggaac gcgccgtcgc
     1321 catcgccctg gacggctcnn nnnnnnnngc ctgatcgtca ccgacggcat ccacgacagg
     1381 ttcgtggccc tgctggccga gaagatcgcc gcgctccgcg tgggcgatgc tctggacgcc
     1441 aacacccaga tcggcccggc ggtctcggaa gaccagatgg aaaccagcta caagtacatc
     1501 gacatcgcca cgggcgaagg cggccgcgtc gtcaccggcg gccagcgcct acagctggaa
     1561 aaccccggct ggtacgtgca gccgaccctg atcgccgaca ccgaccgcaa catgcggatc
     1621 aacaacgagg aagtcttcgg ccccgtcgcc tcgaccatcc gggtcaagga ctatgaggaa
     1681 gccctggatg tcgccaacgg cgtcgagttt ggcctctcgg ccggcatcgc gaccacctcg
     1741 ctgaaatatg cccgccactt ccagcgtcac gccaaggccg gcatgaccat ggtcaacctg
     1801 gccacggccg gcgtcgacta tcacgtgccg ttcggcggtt cgaagagcag ctcctacggc
     1861 gcccgcgagc agggcttcgc ggcggtcgag ttcttcaccc agaccaaaac ctcgtattcg
     1921 tggtcgtaag ccgcatgtcc tcagccatct atcccagcct caagggcaag cgcgtcgtca
     1981 tcaccggcgg cggcagcggc atcggcgccg gcctgaccac cggcttcgtg cgccagggcg
     2041 ccgaggtgat cttcctcgac atcgccgacg aggactctca cgccctggtg gcgcagctgt
     2101 cgaccgacgc ggccatcgcg ccggtctata ggcactgtga cctgatggac ctggaaggcc
     2161 tgaaggcgac gctggccgaa atcggcgacg tcgacgtgct ggtcaacaac gccggcaatg
     2221 acgaccgcca caagctggcc gacatcacgc ccgcctattg ggacaaccgc atcggcgtca
     2281 atctgcgcca catgctgttc gcggcccagg cggtcgcgcc gggcatggcc aaacgcggcg
     2341 gcggggcgat catcaatttc ggctcgatca gctggcacct ggggcttgag gatctggtcc
     2401 tgtacgagac cgccaaggcc ggcatc
//
LOCUS       DAOMEO010000454         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4649_length_2426_cov_2.330662, whole genome shotgun sequence.
ACCESSION   DAOMEO010000454 DAOMEO010000000
VERSION     DAOMEO010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4649_length_2426_cov_2.330662"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLH31_10590"
     CDS             complement(<1..211)
                     /locus_tag="PLH31_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR89685.1"
                     /translation="MRALVVEDDPVVGPDLARALGAGGFVVDLATDGEAASFRGSVED
                     YAVVVLDLGLPHLDGLSVLRRWRAEG"
     gene            complement(212..448)
                     /locus_tag="PLH31_10595"
                     /pseudo
     CDS             complement(212..448)
                     /locus_tag="PLH31_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516734.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
     gene            complement(606..827)
                     /locus_tag="PLH31_10600"
     CDS             complement(606..827)
                     /locus_tag="PLH31_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89686.1"
                     /translation="MPDLVTVTCEEFADLRASLPAITDRYLFDVLGISETTWVKLRKG
                     LPIKRITLERALAKRERWRAMGGAGRIAA"
     gene            1176..>1912
                     /locus_tag="PLH31_10605"
     CDS             1176..>1912
                     /locus_tag="PLH31_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HQR89687.1"
                     /translation="MIHRRQHLAAASALALLFVAGPALAQTTAPSISVAPVGATAPTA
                     QGGANAAVLLYNSVDPRKSVIAATGELGGLEFHDLDGARRAALPGGETYAVDTRDLDG
                     RPVLAVLDRQAGRIRLSAFDFATGQAQAIEARPLMLGYAGEGLCLHKSPRDGSLYAFA
                     TGGEGQLDQWLLYPTAEGKLDGRLVRRLHLSSEAKYCVADDASGALYVSQEAVGIWRY
                     DADPEAEAVPTIVDINRLGRIKGEVGGL"
     assembly_gap    1913..1922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1923..>2426
                     /locus_tag="PLH31_10610"
     CDS             <1923..>2426
                     /locus_tag="PLH31_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HQR89688.1"
                     /translation="AILNGGAEGNYLVAANAEAGDYNVYDRNAGDRFLGAFRIQANGA
                     DAIEGPAGLFGLRAPVGPDLPSGALLVSDDRKVGANTRVLSWQDVVAALKISPGQAAP
                     EATASKLALVQATMETQPVEHDGDAADDPAIFVHPTDSAKHAIIATDKKGGLLVYDLS
                     GKLLQDLP"
BASE COUNT          400 a          858 c          783 g          375 t
ORIGIN      
        1 ggccctcggc ccgccagcgg cgcagcaccg acaggccatc caggtgcggc aggccaaggt
       61 ccagcaccac gacggcatag tcctcgacgc tgcccctgaa cgaagccgcc tcaccgtccg
      121 tggcaagatc gaccacgaac ccgcccgcgc ccagcgcgcg cgccagatca gggccgacga
      181 ccggatcatc ctcgacgacg agcgcgcgca atcagtcttc ctcgagcttg agggtcgcgc
      241 cgctcttggc gtccagctcc agttccagca cccggccgcc cttcgtcagg attttcagat
      301 cgtacttcat ccgacctttg tcctcttcga gttcgacgtc gatcacctcc cccggcgcgc
      361 gctgagcggc gatggcgagt atcttcgtca gcgggaggac gtcaccgcgc gccagggcgg
      421 cgcgcgcgac ctcgtgatta tgcttcttgt cgctggcctg ggctccgccg gccgccaggg
      481 ccagggcgag cggcaacaac aggagcgcta gacgggttct gatcaggggc tgggtcatgg
      541 ggcctacgat tccacaacca tcctgacacg ccgctgacgc gccgcaacat ctggccgtca
      601 agcggtcagg cggcgatcct gccggcgccg cccatggcgc gccagcgttc gcgtttggcc
      661 agggcgcgct ccagggtaat gcgcttgatc ggcaggccct tgcgcagctt gacccaggtg
      721 gtctcgctga tgcccagcac gtcgaacaga tagcgatcgg tgatggcggg caggctggcg
      781 cggaggtcgg cgaactcctc gcaggtgacg gtgacgagat caggcatggc gaggcgtcct
      841 ttcgcgcgcg ggatcgcgat ccaccgggtg ggaagatcgt caggaagact tcagtaacga
      901 caagagcgcc tgaacggcct cggcgaaccg ctggaaccgc gcgtgctcta ccactccatt
      961 gtgacagcga tttgccgaat gtgtcagggg cgacaccatc cgcccccgga acaccggccg
     1021 ggtcgtcatg aacgcgtatc caaacctcgc cataaggcgg ctattcagac gctgcggtag
     1081 cgcgtccagg gcaaatccgt tgatcgtcag cgtggcgtca gcaccgctgc tctagatccg
     1141 gtcgtacgac caagatgcga ccacaggggg acaccatgat ccatcgccgc caacaccttg
     1201 ccgccgccag cgccctggcc ctgctgttcg tcgccggtcc ggccctggcc cagaccaccg
     1261 cgcccagcat cagcgtcgcc cctgtcggcg cgaccgcgcc caccgcgcaa ggcggcgcca
     1321 acgcggccgt cctgctctac aacagcgtcg atccccgaaa gagcgtcatc gccgcgaccg
     1381 gcgaactggg cgggctggag tttcatgacc tggacggcgc ccgcagggcc gccctgcccg
     1441 gcggcgaaac ctacgccgtc gacacccgcg atctcgacgg ccgccccgtg ctggcggttc
     1501 tggatcgcca ggccgggcga attcgcctgt ccgcttttga cttcgccacc ggccaggccc
     1561 aggccatcga ggcacgccca ctgatgctgg gctatgccgg tgagggcctg tgcctgcaca
     1621 agagtcctcg cgacggctcg ctctatgctt tcgccaccgg tggcgagggt cagctggacc
     1681 agtggctgct gtaccccacc gccgagggaa aactggacgg ccgcctggtg cgccgcctgc
     1741 acctgtcgtc ggaagccaag tactgcgtcg ccgatgacgc cagcggcgcg ctatatgtct
     1801 ctcaggaagc ggtcggcatc tggcgctatg acgcagatcc cgaggccgag gccgtgccga
     1861 ccattgtcga catcaaccgc ctgggccgca tcaagggcga agtcggcggt ctnnnnnnnn
     1921 nnggccatcc ttaacggtgg ggcggagggc aattatctgg tcgccgccaa tgccgaggcg
     1981 ggcgactaca acgtctatga ccgcaatgcc ggggacaggt tcctgggcgc cttccgcatc
     2041 caggccaacg gcgccgatgc gatagagggc ccggccggcc tgtttggcct gcgtgcgcct
     2101 gtcggccccg atctgccgtc cggcgccctg ctggtctccg acgaccgcaa ggtgggcgcc
     2161 aacacccggg tgctgtcgtg gcaggacgtc gtcgccgccc tgaagatctc acccggccag
     2221 gccgcgcccg aggcgacggc cagcaagctg gccctggtgc aggcgaccat ggagacccag
     2281 cctgtcgaac acgacggcga cgcggccgat gatccggcga tcttcgtcca tccgactgac
     2341 tcggccaagc acgccatcat cgccacggac aagaagggcg gcctgctggt ctatgacctt
     2401 tcgggcaagc tgctgcagga cctgcc
//
LOCUS       DAOMEO010000455         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4664_length_2420_cov_2.413953, whole genome shotgun sequence.
ACCESSION   DAOMEO010000455 DAOMEO010000000
VERSION     DAOMEO010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4664_length_2420_cov_2.413953"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLH31_10615"
     CDS             complement(<1..492)
                     /locus_tag="PLH31_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89689.1"
                     /translation="MRTGHILKTRLSEYGALWLACFVLVLAGVGFVTFALGRDLITVA
                     DMVLPISFMILGLAVAVGVGITVASPASLIAKCLVTLLALLLILPLLWSPVVAVLIIA
                     AISQVPIEYSEAYAQFRISVSHLIYPVVAMLVEGPLVAAVWNAFLIVASIVIALLLVA
                     SKEA"
     gene            612..1376
                     /locus_tag="PLH31_10620"
     CDS             612..1376
                     /locus_tag="PLH31_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQR89690.1"
                     /translation="MADTGEPQTLCEPHASRGELISNRLLGALRPRELERLSPYLRKV
                     DLARDTMLFDCGEDVTLTHLPCHATMVSLLVVSKEGQEVEVANVGREGAIGGIVSAGF
                     KPAYGRAVVRVPGFAFCIPTARLEDAKSQSPILADLFARYADVLLAQMMQSAACNALH
                     GVEQRYCRWLLSTHDRAGDEPIRLTQETLAQMMGVQRTTVTAVARALQDDGVIATGRG
                     RIEILDRSALEKRACECHDAVEHHFQRLLPEVDTTD"
     gene            complement(1366..2331)
                     /locus_tag="PLH31_10625"
     CDS             complement(1366..2331)
                     /locus_tag="PLH31_10625"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQR89691.1"
                     /translation="MALHTPLCDLLAIEHPILLAGMGGVSYAPLAAAVSNAGGYGVLG
                     MAGTTPDFIRDQMRQVRALTDKPFGVDLLAATPDALIDSVEVIIAEGASSFIAGLGVP
                     MPIIERLKSAGLKVMVVCGAVKHAVKAEQAGCDAVICQGGEGGGHTGLVGTLPLVAQA
                     VDAVKIPVVAAGGLHDGRGLAAALALGAQGVWMGTRFIASTEAHAGDLYRQAVVEAAD
                     EDTVRTRCYSGKPMRVKKNPYVEDWESRPGDIQGFPQQAMVSIRAGAMGGIGGQIEGL
                     DADRSCFAMGQSAGGIREVLPAGVIVEQLVTEARAAISRIAALNR"
BASE COUNT          426 a          852 c          783 g          359 t
ORIGIN      
        1 cgcctctttg ctcgccacga gcagcaaggc gatcacgatc gaggcgacga tcaggaaggc
       61 gttccagacg gcggcgacca gcggcccttc caccagcatc gccaccaccg gatagatcag
      121 gtggctcacc gagatccgga actgggcata ggcttccgaa tattcgatcg gaacctgact
      181 gatggcagcg atgatcagca cggccacaac cggcgaccac agcagcggca ggatcaggag
      241 cagcgccagc agggtgacca ggcacttggc gatcaggctg gcgggcgagg ccacggtgat
      301 tccgacgcca accgccaccg ccaggcccag gatcatgaag ctgatcggca agaccatgtc
      361 ggcgacggtg atcaggtccc gccccagagc gaaggtgaca aagccaacgc cggccaggac
      421 cagtacaaag caggccagcc acagggcgcc atactcgcta agccgcgttt tcaggatatg
      481 gccggttcgc atgccgctct ccccatcgac ggcaagcagc ctgcctccta agcttagccg
      541 cgtcgatggg aaactaaagc gccgcttcgc gcgctatggc caagcacgct tgcgatcgcg
      601 gaggatcgat catggccgat acgggtgaac ctcaaacgct atgcgagcct catgcctcgc
      661 gcggcgaact catctccaac cgcctgctgg gcgcgctgcg accacgcgaa ctggagcggc
      721 tgagccccta tctgcgcaag gtggatctgg cgcgcgacac catgctgttc gactgcggcg
      781 aggatgtcac cctgacgcac ctgccctgtc acgccaccat ggtctcgctc ctggtcgtct
      841 ccaaggaagg ccaggaggtc gaggtggcca atgtcggccg cgaaggcgcc atcggcggca
      901 tcgtcagcgc gggcttcaag ccagcctatg gtcgcgccgt ggtccgcgtg ccgggcttcg
      961 ccttctgcat cccgacagcc cggcttgaag acgccaagag ccagtcgccg atcctggcgg
     1021 acctctttgc ccgctatgcc gacgtgcttc tggctcaaat gatgcaatcg gcggcctgca
     1081 acgccctgca cggggttgag cagcgttact gccgctggct tttgtcgacc cacgatcggg
     1141 ctggcgacga gccgatccgc ctgacccagg agaccctggc ccagatgatg ggcgtgcagc
     1201 gcacgacggt caccgccgtg gcccgcgccc tgcaggacga cggcgtgatc gcgaccggcc
     1261 gcggacgcat cgagatcctc gaccggtcag ccctcgagaa acgggcctgc gaatgccacg
     1321 acgccgtcga gcatcatttc cagagactgt tgcccgaggt cgatactacc gattgagcgc
     1381 ggcgatccgg ctgatcgcgg cgcgagcctc ggtgaccagc tgttcgacga tcacgcccgc
     1441 cggcagcacc tcgcggatgc cgcccgcgct ctggcccatg gcgaagcagg atctgtcggc
     1501 atccaggcct tcgatctggc cgccgatgcc gcccatggcg ccggcccgga tcgagaccat
     1561 cgcctgctgc ggaaagccct ggatgtcgcc gggccgggat tcccagtcct cgacataggg
     1621 gttcttcttc acccgcatcg gcttgcccga atagcagcgg gtgcggacgg tgtcctcgtc
     1681 ggcggcctcg accaccgcct ggcgatacag gtctccggca tgggcctcgg tggaggcgat
     1741 gaaccgcgta cccatccaga cgccctgggc gcccagggcc agggccgcgg cgagccctcg
     1801 cccgtcatgc agaccgccag cggcgaccac cgggatcttc acggcgtcca ccgcctgggc
     1861 cacgaggggc agggtgccga ccaggcccgt atggccgccg ccttcaccgc cctgacagat
     1921 caccgcgtcg cagccggcct gctcggcctt gaccgcatgt ttcacggccc cgcagacgac
     1981 catgaccttc aggcccgccg acttcagccg ctcgatgatc ggcatcggta cgcccaggcc
     2041 agcgatgaag gacgaggcgc cctcggcgat gatgacctcg acgctgtcga tcagggcgtc
     2101 cggcgtggcg gccagcagat cgacgccgaa cggcttgtcg gtcagcgcgc ggacctggcg
     2161 catctggtcg cggatgaagt ccggcgtcgt gccggccatg cccagcacgc cgtagccgcc
     2221 ggcgttcgac acggccgccg ccagcggggc gtaggagacg ccgcccatgc cggccagcag
     2281 gatcggatgc tcgatagcca gtagatcgca aaggggcgta tgcagcgcca tggaacccgt
     2341 cctcccgtta ttttccggca ggatgggcgc ggcggcgacc ggctcgcaag cctgccgcga
     2401 ggtcagccgc cgaaccctcc
//
LOCUS       DAOMEO010000456         2418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4668_length_2418_cov_2.162505, whole genome shotgun sequence.
ACCESSION   DAOMEO010000456 DAOMEO010000000
VERSION     DAOMEO010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4668_length_2418_cov_2.162505"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..716
                     /locus_tag="PLH31_10630"
     CDS             18..716
                     /locus_tag="PLH31_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HQR89692.1"
                     /translation="MSLESAARLTEALLALALLQQSLEHLRGSRPERTLFVARMALCG
                     LVLLGAAFTWPWVGLIGLVGLAGLSLLILRRFQGPYNGGSDRMGLLALWCLTLSRLAP
                     TPALAELALGYLGLQLMLSYFISGGVKIVNPDWRSGRALADVFRFSAYPVSEDLRRLA
                     DRPRLLLALSWAVMLFELAFPLTLLSRESLIVGLIVAGTFHLANACLFGLNRFFWTWL
                     SVYPAILWLQARLV"
     gene            complement(780..>2418)
                     /locus_tag="PLH31_10635"
     CDS             complement(780..>2418)
                     /locus_tag="PLH31_10635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HQR89693.1"
                     /translation="QVKAVKTKDDFTALMGKSSVTPFTSLLGIGIQTDAKNPKAYAVY
                     AGSGGLSLPNRDYYLEARFAAQKAAYQVYVEKILTMAGWDKPAANAKAIVDFETKLAE
                     VTWTRAELRDRDKTYNPATLAELQAMTPGYDWNRYLAGSELPKVDRFIVTTNTTFPKY
                     AKIYAETPLDTLKAWQAFKVADGAAPMLSKRFVDASFEFRNKTLSGQPEQRPRWKRGV
                     AQVNGMLGEAVGKDYVAAYFPPESKAKMLELVANVRSAMKVRIDNLDWMSAETKVKAQ
                     DKLAKFTVKIGYPEVWEDYSKLEIKDDDAFGNAQRAGVWAWRKDVERLFKPVDKTEWG
                     MTPQTVNAYYNSVNNEIVFPAAILQAPFFHPDADPAINYGGIGGVIGHEIGHGFDDQG
                     RKSDGDGVLRDWWTAEDAAKFKAQADRLGAQYGAFEPLPGLKLNGPLTMGENIGDMGG
                     LAFGLDAYHASLGGKPAPILDGLTGDQRVYLGWAQVWRQKQREEALKQQVTTGPHSPA
                     YYRVNGTIRNQDGWYKAFDIKPGDKLYIAPEDRVRIW"
BASE COUNT          325 a          768 c          832 g          493 t
ORIGIN      
        1 gcggacatca ccgtgcgatg agcctggaga gcgccgcccg gctgaccgag gccctgctgg
       61 ccctggccct cctccagcag agcctggagc atctccgtgg cagccgcccc gagcggacgc
      121 tgtttgttgc gcgaatggcg ctgtgcggcc tcgtgctgtt gggcgcggcc ttcacctggc
      181 cgtgggtcgg cctgatcggc ctggtgggtc tggccgggct gtcgctgctg atcctcaggc
      241 gcttccaggg cccttataat ggcggaagcg acaggatggg cctgctggcc ctgtggtgtc
      301 tgaccctgtc gcgcctggcg cccacaccgg ccttggccga gttggccttg ggctatctgg
      361 gcctgcaact gatgttgtcc tactttattt ccggtggggt gaagattgtg aaccccgact
      421 ggcgcagcgg gcgggccctg gccgatgtgt tccggttctc ggcctatccc gtcagcgagg
      481 acctgcgccg cctggccgat cgaccgcgcc tgttactggc cctgtcctgg gcggtcatgc
      541 tgttcgagct ggcctttccg ctgaccctgc tgtcgcgcga gagcctgatc gtcggactga
      601 tcgtggccgg gacgttccac ctggccaatg cctgcctgtt tggcctgaac cgctttttct
      661 ggacctggct gtcggtttac ccggcgatcc tgtggctaca ggcgcggctg gtgtgaaaga
      721 aaaaggggct ggcgcctgag cgccagcccc ctcgtcgttg ccgcctgacg atcagtcgat
      781 caccagatcc tcacgcgatc ttccggggcg atgtacagct tgtcgcccgg cttgatgtcg
      841 aaggccttgt accagccgtc ctggttccgg atcgtgccgt tgacgcggta ataggccggc
      901 gagtgcgggc cggtggtgac ctgctgcttg agggcctcct cgcgctgctt ctggcgccac
      961 acctgggccc agcccagata gacgcgctgg tcgccggtca ggccgtccag gatcggcgcc
     1021 ggcttgccgc ccagcgaggc gtgataggcg tcgagaccga acgccaggcc gcccatgtcg
     1081 ccgatgttct cgcccatggt cagcggaccg ttcagcttca ggcccggcag cggctcgaag
     1141 gccccgtact gggcgcccag gcggtcggcc tgggccttga acttggccgc gtcctcggcg
     1201 gtccaccagt cgcgcagcac gccgtcgccg tcggacttgc ggccctggtc atcgaagccg
     1261 tggccgatct cgtgaccgat cacgccgccg atgccgccat agttgatggc cgggtcggcg
     1321 tccgggtgga agaacggcgc ctgcaggatg gcggcgggga agacgatctc gttattgacc
     1381 gagttgtagt aggcgttgac cgtctgcggg gtcatgcccc actcggtctt gtcgaccggc
     1441 ttgaacaggc gctcgacgtc cttgcgccag gcccagacgc ccgcgcgctg ggcgttgccg
     1501 aaggcgtcgt catccttgat ctcgagcttg gaatagtctt cccagacctc ggggtagccg
     1561 atcttgacgg tgaacttggc cagcttgtcc tgggccttga ccttggtctc ggcgctcatc
     1621 cagtcgaggt tgtcgatccg caccttcatg gccgagcgga cattggccac cagctccagc
     1681 atcttggcct tggactccgg cgggaaatag gcggcgacat agtccttgcc gaccgcctca
     1741 cccaacatgc cgttgacctg ggccacgcca cgcttccagc gcgggcgctg ctcgggctgg
     1801 cccgacaggg tcttgttgcg gaactcaaag ctggcgtcga cgaaccgctt ggagagcatg
     1861 ggcgccgcgc cgtcagcgac cttgaacgcc tgccaggcct tcagggtgtc cagcggggtt
     1921 tcggcataga tcttggcgta cttcgggaag gtcgtgttgg tggtgacgat gaagcggtcg
     1981 accttgggca gctccgagcc ggccagatag cggttccagt catagcccgg cgtcatggcc
     2041 tgaagctcgg cgagcgtggc gggattatag gtcttgtcgc gatcgcggag ctcggcgcgg
     2101 gtccaggtga cttcggccag cttggtctcg aaatcgacga tggccttggc gttggcggcg
     2161 ggtttgtccc agccggccat ggtcaggatc ttctcgacat agacctggta ggcggccttc
     2221 tgggcggcga acctggcttc cagatagtag tcgcggttgg gcagcgacag gccgcctgag
     2281 ccggcataga cggcgtaggc cttggggttc ttggcgtcgg tctggatgcc gatgcccagc
     2341 aggctggtga acggcgtgac gctgctcttg cccatcaggg cggtgaagtc gtccttggtc
     2401 ttcacggcct tgacctgg
//
LOCUS       DAOMEO010000457         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4676_length_2416_cov_2.579839, whole genome shotgun sequence.
ACCESSION   DAOMEO010000457 DAOMEO010000000
VERSION     DAOMEO010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_2416_cov_2.579839"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..2352
                     /locus_tag="PLH31_10640"
     CDS             268..2352
                     /locus_tag="PLH31_10640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR89694.1"
                     /translation="MIGFLVSATVAQAQETAPPPQAAPPAQTSVAPEGPRPYTSLRRR
                     PRPATPRRAVAPRPAVAPVAPIAPPIPGVTPLPPAELEAFVDGLIRRSMVEDHIAGVT
                     IAVVQNGQVVLKKGYGVASLKERRAVDPDRTLFRIASISKTFTWIALMNQIEAGRMRL
                     DGPVNLYLPEPLQVRGKDMAPVLLRHLMTHTPGFEDRAMGQLFEQDWKRVRPLNEYLR
                     QERPRRVREPGGLPTYSNYGVALAGAAVANVTGKPFEQLVAEEIIFPAGMTHTTFRDA
                     RPWRDGLPAPMAPALAADRSAGFRWTQLGWREQPPEMTGQVAPAGGASSTAADMSRYM
                     MLLLGNGTIDGKTIYSPRTAQAFRTPMYQPAPEAAGWNAGFQDIALPGGRRGYGHNGA
                     TLWFFSNMVVVPDLGLGVFIAVNTDTGADLPSVVPTAIIERFYAPAPAVPVTRPLSPE
                     AARIYEGDYLGTRRAYGGLEGFIGRITQRAEVRVTPDGQLALLTDGRSTLWNATEKPG
                     VFQASDSAKTLVFETVGGRGVRFYPSPGFSAFERISFPMGAGLLIWIVALSAFAAVAT
                     LAGVFMRDRRETRQTPTQTRANLLQTTQATLWLIALCCVGVFAAKSDDIAWVFYGWPS
                     GWLVTASACALVASALTAVTLIMAPVVWRGGRRVDSWTTLRKLAFTYTALLYAVLGLL
                     LAYWNFLLPVKG"
BASE COUNT          343 a          893 c          768 g          412 t
ORIGIN      
        1 gcccttcgcc cggttcaaga cctaggacct gacgatatcg agccggcggg cctggatggg
       61 tccctgtcct gcatcgttcc tgcccgcgga ctccgtttca aggcgcaagg acggggcgcc
      121 ctgacaacgt ccccagcaat agacgtcaat cgcgcacgcc gccctttgaa cgcggtgcga
      181 ggcgccctag aagtcggctc agtcattcag cctcgggggt cggccttgac gtcgcgcttt
      241 tcgtccggtg ccgcgcttgt tgcggccatg atcggttttc tggtttcggc gactgtcgcc
      301 caggcccagg agaccgcgcc gccgcctcag gccgcgcccc cggcgcagac ctctgtcgcg
      361 cccgaaggtc ctcgccccta taccagcctg cgacgtcgcc cccggccggc gacgccccga
      421 cgggccgttg cgcccaggcc cgccgtagcc ccggtggctc ccatcgcgcc gcctatcccc
      481 ggggtcacac ccctgccccc tgcggagctg gaggcttttg tcgacggcct gatccgtcgc
      541 agcatggtcg aagaccacat cgccggcgta accatcgccg tcgtgcagaa cggccaggtg
      601 gtgctgaaaa agggctatgg cgtcgccagc ctgaaggagc gccgcgcggt tgacccggac
      661 cggacgctgt tccgcatcgc ctcgatctcc aagacgttca cctggatcgc cctgatgaac
      721 cagatcgagg ccgggcggat gcgcctggac ggtccggtca atctgtacct gcccgagccg
      781 ctgcaggtgc gcggcaagga catggccccg gttctgcttc gccacctgat gacccacacg
      841 cccgggttcg aggatcgcgc catgggccag ttgtttgagc aggactggaa gcgcgtacgg
      901 ccgctgaacg aatacctgcg tcaggagcgc ccccgccgcg tgcgcgagcc cggcggtctg
      961 ccgacctatt ccaactatgg cgtggccctg gccggcgcgg ccgtggccaa tgtcaccggc
     1021 aagcccttcg agcagttggt cgccgaagag atcattttcc cggccggcat gacccacacc
     1081 accttccgcg acgcccgccc ctggcgtgac ggcctccccg cgccgatggc cccggccctg
     1141 gccgccgacc gctcggcggg cttccgctgg acccagctgg gttggcgcga gcagccgccc
     1201 gaaatgaccg gccaggtcgc tcccgccggc ggcgcctctt cgacggcggc cgacatgagc
     1261 cgctacatga tgctgctgct gggcaacgga acgatcgacg gcaagaccat ctattcgccg
     1321 cgcacagccc aggccttccg cacaccgatg taccagcccg cgccagaggc ggccggctgg
     1381 aacgccggct tccaggacat cgccctgccc ggcggccggc gcggctatgg ccacaatggc
     1441 gcaacccttt ggttcttctc caacatggtc gtggttcctg accttggcct gggcgtgttc
     1501 atcgccgtga acaccgacac cggcgccgac ctgccgtcgg tggtgccgac cgcgatcatc
     1561 gagcggttct atgcgccggc gcccgccgtg ccggtcacgc gccccctcag cccggaagcg
     1621 gcccggatct atgaaggcga ctatctgggc acacgccgcg cctatggcgg cctggagggc
     1681 ttcatcggcc gcattaccca gcgggccgaa gtgcgcgtca cccccgacgg gcagctggcc
     1741 ctactgaccg acggccgttc caccctgtgg aacgccaccg aaaagcccgg cgtgttccag
     1801 gccagtgaca gcgccaagac cctggtcttc gaaaccgtcg gcgggcgcgg ggtgcggttc
     1861 tatccgtctc ccggtttctc cgccttcgag cgcatcagct ttcccatggg cgctggtctg
     1921 ctgatctgga tcgtggccct ctcggccttc gccgccgtcg ccaccctggc gggcgttttc
     1981 atgcgcgacc gtcgcgagac ccgccagacc ccgactcaga cgcgggccaa ccttctgcag
     2041 accacgcagg cgaccctgtg gctgatcgcc ctgtgctgcg ttggggtgtt cgccgccaag
     2101 tcggacgaca tcgcctgggt cttctatggc tggcccagcg gctggctggt gaccgcctcg
     2161 gcctgcgccc tggtggccag cgcgctgacc gccgtcaccc tgatcatggc gccggtcgtc
     2221 tggcgcggcg ggcgccgggt cgacagctgg accacgctgc gcaaactggc cttcacctac
     2281 accgccctgc tctatgccgt tctgggcctt ttgctggcct actggaactt cctgctgccc
     2341 gtgaaaggct aaagtcgccc ggcgctgaaa ttgcactgtt ctcccatagg aagtccttgg
     2401 gaggggatga cgtgtc
//
LOCUS       DAOMEO010000458         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4677_length_2416_cov_1.945362, whole genome shotgun sequence.
ACCESSION   DAOMEO010000458 DAOMEO010000000
VERSION     DAOMEO010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_2416_cov_1.945362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLH31_10645"
     CDS             complement(<1..239)
                     /locus_tag="PLH31_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89695.1"
                     /translation="MGLNTDTAAKLRKLAADESLSDTALLNRSLMLSVKWITQLWVNT
                     LVNESVAIRAGVFEGMKYVVNAAEGALLPRLVGTY"
     gene            complement(404..>2416)
                     /locus_tag="PLH31_10650"
     CDS             complement(404..>2416)
                     /locus_tag="PLH31_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQR89696.1"
                     /translation="RGSQAQMKQLGGMRGLMAKPSGEIIETPIISNFKEGLTVQEYFN
                     STHGARKGLADTALKTANSGYLTRRLVDVAQDCIIVEEDCGTTRGITLRAVVEGGDVL
                     VSLGMRVLGRFSAEDIKDPATGELVVPADTYLDENMADAIEAAAVQSVKVRSVLTCEA
                     KVGVCGACYGRDLARGTPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGTAQVAEQSF
                     FESSNDGTVRVTGATVTGADGFLVVMSRNTAVSVLVDGKERENYKPPYGARLKIKDGE
                     KVKRGQRLADWDPYTTPIITEVAGKIRAEDLVDGFSVREETDEATGIAQRVIADWRTS
                     ARASDLRPAMGVLAEDGSYVRLANGGEARYLMSVGAILSVADGDMVKPGEVIARIPTE
                     GAKTRDITGGLPRVAELFEARRPKDCAVIAEMDGRVEFGKDYKNKRRIKITPEVDADG
                     NQGEPVEFLIPKGKHIAVHDGDFIARGEYIIDGNPDPHDILRILGVEALANFLVDEIQ
                     EVYRLQGVPINDKHIETIVRQMLQKVEILEPGDTGLIKGDHLDKPEFYIEQDRAVARG
                     GRPATTQPVLLGITKASLQTKSFISAASFQETTRVLTEASVHGKTDTLEGLKENVIVG
                     RLIPAGTGSYLRSLQRVAAKRDEQLAQQREDAMEPLPEAIALADAE"
BASE COUNT          385 a          776 c          787 g          468 t
ORIGIN      
        1 tcataggttc ccaccaggcg cggcagcagg gcgccctcgg cggcgttgac cacgtatttc
       61 atgccttcga agacaccggc ccggatcgcc acgctctcat tgacgagggt gttgacccac
      121 agctgcgtga tccatttcac agacagcatg agcgagcggt tcagcagcgc agtgtccgac
      181 aggctctcgt ccgccgcgag cttgcgcagc ttggcggcgg tgtctgtgtt caggcccata
      241 cgcgcttccc agattgccgc ccctgatgaa cgttccaggg aaacgaaaaa ggcccgggat
      301 tggtcccggg cctttctcat tctttcgatc ccgaagacgg cgtcaaccat ccctggaagc
      361 ctgaacgccg gttggcgtca gacctcctga agatcgctta cgcctattcg gcgtccgcaa
      421 gggcgatggc ctcgggcaga ggttccatcg cgtcttcgcg ttgctgggcc agctgctcgt
      481 ctcgcttggc ggcgacgcgc tgcaggctgc gcagatagga acccgtaccg gcggggatca
      541 gacgaccaac gatgacgttt tccttcaggc cttccagggt gtcggtcttg ccgtgcaccg
      601 aggcttcggt caggacgcgg gtcgtttcct ggaacgacgc ggccgagatg aagctcttgg
      661 tctgcaagct cgccttggtg ataccgagca gcacgggctg ggtggtggcc ggacggccgc
      721 cacgcgcaac cgcgcggtcc tgctcgatgt agaactcggg cttgtccagg tgatcgccct
      781 tgatcagacc cgtgtcgccc ggctcgagga tctcgacctt ctgcagcatc tgacgaacga
      841 tcgtctcaat gtgcttgtcg ttgatcggca cgccctgcag tcgatagacc tcctggattt
      901 cgtccacgag gaagttggcc agggcctcga cgcccaggat gcgcaggatg tcgtgcggat
      961 ccgggttgcc gtcgatgatg tattcgccgc gcgcgatgaa atcgccatcg tgcaccgcga
     1021 tgtgcttgcc cttggggatc aggaactcga ccggctcgcc ctggttgccg tcagcgtcga
     1081 cttccggggt gatcttgata cggcgcttgt tcttgtaatc cttgccgaat tcgacgcgac
     1141 cgtccatttc cgcgatgacc gcgcagtcct tgggacggcg ggcttcgaag agttcggcga
     1201 cgcgcggcag accgccggtg atgtcgcggg tcttggcgcc ttcggtcggg atccgcgcga
     1261 taacctcgcc cggcttgacc atgtcgccat cggccaccga gagaatggcg ccgaccgaca
     1321 tcaggtagcg agcctcgccg ccgttggcga ggcggacata ggagccgtct tcagccagaa
     1381 cgcccatggc cggacgcagg tccgaagcgc gggccgaggt ccgccagtcg gcgatcacgc
     1441 gctgcgcgat accggtggct tcgtcggttt cctcgcggac ggagaagccg tccaccagat
     1501 cttcggcgcg gatcttgccg gccacttcgg tgatgatcgg ggtggtgtag gggtcccaat
     1561 cggccagacg ctgaccgcgc ttgaccttct cgccgtcctt gatcttcagg cgcgcgccgt
     1621 aaggcggctt gtagttctcg cgttccttgc cgtcgaccag gacgctcacg gccgtgttgc
     1681 ggctcatgac gaccaggaag ccgtcagcgc ccgtaaccgt ggcgccggtg acgcggaccg
     1741 taccgtcgtt gctgctttcg aagaacgact gctcggccac ctgggcggtg ccgccgatgt
     1801 ggaaggttcg catcgtcagc tgggtgcccg gctcaccgat ggattgggcg gcgatgacgc
     1861 cgaccgcttc accgatgttg accggggtgc cccgggccag gtcgcgacca tagcaggcgc
     1921 cgcagacgcc gaccttggct tcacaggtca ggaccgaacg caccttcacc gactgcacgg
     1981 cagcggcttc aatggcgtca gccatgttct cgtcgagata ggtgtcggcg ggaacgacca
     2041 gttcgccggt cgccggatcc ttgatgtcct cggccgagaa gcggcccagg acgcgcatgc
     2101 ccagcgagac gaggacgtcg ccgccctcga ccacggcgcg cagggtgatg ccccgcgtgg
     2161 tgccgcagtc ttcctcgacg atgatgcagt cctgcgccac gtcgaccaga cgacgcgtca
     2221 ggtaacccga gttggcggtc ttcagcgcgg tgtccgccag gcccttacgg gcgccgtggg
     2281 tcgagttgaa gtactcctga acggtcaggc cttccttgaa gttcgagatg atcggggttt
     2341 caatgatctc acccgagggc ttggccatca gaccgcgcat gccgcccagt tgcttcatct
     2401 gggcttgcga accacg
//
LOCUS       DAOMEO010000459         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4684_length_2414_cov_2.396354, whole genome shotgun sequence.
ACCESSION   DAOMEO010000459 DAOMEO010000000
VERSION     DAOMEO010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4684_length_2414_cov_2.396354"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2414)
                     /locus_tag="PLH31_10655"
                     /pseudo
     CDS             complement(1..2414)
                     /locus_tag="PLH31_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518692.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
BASE COUNT          320 a          826 c          917 g          351 t
ORIGIN      
        1 gcccgtcggc caggcggcga tgaaggccgg cgagtggaag ctgccccgcc atggcctggg
       61 cgcccaggcc gtagcccttg cgggtcacca ccgtgaacag cggcgagcgc agcttggccc
      121 cggcgatgaa ctgccgactg acgcggcgca cggcggcggc gtcttcgttg cccggcccca
      181 ccatgaagcc gggggtgtcg cacaggctga gcaccggcag ggcgaaggcg tcgcacagct
      241 gcaggaaccg cgcggccttc tcggccccgt cgcagtcgat ggccccgccc agatggcgcg
      301 gatcattggc gatcaggccc atggggcggc cctcgatgcg gatcaggccg gtgaccatgc
      361 cggccgcgaa gccgccgcgc agctccagaa acgaggcgtc gtcgaccagt ccggcgatca
      421 gggcgcgcac gtcatagacc cgcaggcggt tctcggggat cgcgccccgc agccgccgct
      481 ggtcaggcgc ggtccaggcg gtgagcgttc cctggaaata ggacagggcc tgcttggcca
      541 gccgcgtggc gtgggcctcg tcgtcggcca ggatgtcgat gacgccattg acccattggt
      601 gcgccgaagg gccgatctgt tcgggcctga agacgcccag gccgccgccc tcgatcatgg
      661 cggggccgcc catgcccaga ttgctgtcgc gggtggcgac gatgatttcc gaaagcccgg
      721 cgatggcggc gttgccggcg aagcagcggc ccgcgacgat ggcgatcttg ggcacgatgc
      781 cggaaagctc tgcgaagctg cggaaggtcg gtacgtccag ccccgccacg gccgtggtgt
      841 cggtgtcgcc cggccgtccg ccgccgcctt cggcgaacca gacgacgggc agcttctggt
      901 cggcgatcac cgccatcaac cggtcggtct tgcggtggtt catcgcgccc tgggtaccgg
      961 ccagcacggt gaaatcatag gccagggccg cgcagcgcgc cttgtctgcg gggaagaggc
     1021 cgccattgac cgtgccgatg ccggtgatca ggccatcggc cggcgtgttg gcgatcaggt
     1081 cctcggtggt gcgccggcgc ttctgggcgg cgatggccag ggcgccgtat tcgaggaagc
     1141 tgccgggatc gaccagatcg tcgatgtttt cccgcgccgt gcggtggccg gtcttgcgac
     1201 gccgggcgac ggcttcgggg cgagcctcgt cgagggtgaa gcggtggcgg gcgatgactt
     1261 cggcgaggtc ggggcggatc gcatccagat cctcggcggc cgcgtcggcg gcgatggcag
     1321 cgtccatctg caccggttcg aggaacagca gcgggtggcc ctcggccacg gtctggcccg
     1381 gcgcgacagc gatcttcagc acccgaccgc cggtctgggc gagcacgagg tgctccatct
     1441 tcatggcttc cagcacggcc aggggctgtc cggggcggac cagatcgccc tcggcgacct
     1501 cgacggcgcc gaccgtggcg gccaggggcg cggcgatggt cagcgcgcca tccagggtct
     1561 cgaagtgggc ttcggcggcg ctttccacag acacggggag gttggcgacc agctcggcgg
     1621 cgtggtcgtc gatgaaccgg gtggtggccg cgccgtcttt ccagtccgcc tgcgccagga
     1681 tcgcgcggat cagcggcgcg gtggtggcga cgccggtgat cgtgaactcg cccagggccc
     1741 ggtccagacg ggcggcggcc tcggccgggg tgcgaccgcg cgcgatcacc ttggccagca
     1801 ggctgtcata ggccccgccc accgtcaggc cggccgcgcc cgcgccatcc acccgcacgc
     1861 cgggaccgcc cggcggcgca taggcgttga gggttccgga cgagggcttg acctgaccgt
     1921 cggatgtcag ggtttcggcg ttgatccgcg cctggatcgc cgcgccgacg gcgggcgggg
     1981 cggtggtcag gtccagttcg gccagggtct tgccggctgc cagttcgaac tgcaggcgca
     2041 ccagatccag gcccgtgacc tcttcggtgc cgcctgggcc atggcggcgc gggcggcgcc
     2101 cggcaggttc ggggctggag cgatctcgac caccttctgg ttgcggcgct gcagggagca
     2161 gtcgcggtcg ccgaccgcca ggacgcgggc gccgtcgccg acgatctgca cctcgatatg
     2221 gcgcgcccgt tcgatcagct tttcggcata gaggccgtca tcgccgaacg cggccatggc
     2281 ctcgcgggcg caggcggcga aggccgggcc cagttcgccg gggtcgagca ccagccgcat
     2341 gccgcgtccg ccgccgccgg cccgggcctt cagcatgatc gcgccgtgct ggtcgaagaa
     2401 ggcctctgcg gcgg
//
LOCUS       DAOMEO010000460         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4687_length_2413_cov_2.667091, whole genome shotgun sequence.
ACCESSION   DAOMEO010000460 DAOMEO010000000
VERSION     DAOMEO010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_2413_cov_2.667091"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2343)
                     /locus_tag="PLH31_10660"
     CDS             complement(<1..2343)
                     /locus_tag="PLH31_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HQR89697.1"
                     /translation="MNVEDFITRWTAREGGAERANYQMFLSELCDVLGVPRPDPAGAA
                     RDLNDYVFERAVSSTTLDGASAKRIDLYKRAAFVLEAKQTHAPGAKKAAPDQLSLPGA
                     DAEPEQLGRRSTAKGWDVLMRKAFNQANDYVHLLPTDHAAPPFLIVADVGHCFEIYAD
                     FSGTGRGYAYYPDRKGFRIYLDELRDPRLRARLATIWTDPHSLDPTRESARVTRQIAE
                     RLAAVSKALEAKGARAEDVALFLMRCIFTMFAEDVELLPKGAFTQLLRKSVETPERFP
                     QRVKELWTAMDTGGESMAIDARVLHFNGGLFKQTTAFELGREEIGELLVAASHNWREV
                     EPAIFGTLVEQALDPNERRRLGAHYTPRAYVERLVNVTVMEPLRADWRAVRTKAEALA
                     DAGDEKAAREAVRAFHHQLCATRVLDPACGTANFLYVSLELMKTLEAEVLETLVQLGE
                     AESMGFETVDPHQFLGIELNPRAAAIAELVLWLGYLQQHYRSRSGHPAEPILKAFHNI
                     ENRDAVLTWDGYPLTTVVEKDGVRVETYPNARRPEWPEAEFIVGNPPFIGGSTLRSRM
                     PPGYAEALWAAHKGMNDSADFVMYWWDRSAEILARKKSILRRFGFVTTNSITQVFQRK
                     VMERHLNGKPPISLIFAIGDHPWTKAGRESAAVRIAMTAAARGKLEGVAWEVASETGL
                     DTDEPVLTFTEKRGKVNSDLTVGYDLTRAKGLAANKGLASRGLQLMGGGFIVTPEEAA
                     HLGLGQRPGLEKHIRLYRNGRDLTSRPRGVMVIDLFGVPAD"
BASE COUNT          393 a          788 c          808 g          424 t
ORIGIN      
        1 atccgccggc acaccaaaga ggtcgatgac cattacgccg cgcggtcgag aggtaaggtc
       61 tcgaccattg cggtaaagtc ggatgtgctt ctctagcccg ggtcgctgac ctaagcctag
      121 gtgcgcggct tcctcaggag ttacgataaa cccgccgccc atgagttgga ggccccgaga
      181 ggctagcccc ttattggccg ccagaccttt cgcacgggtc agatcataac ctaccgtgag
      241 gtcagagttc accttgcctc gcttctccgt gaaggtcagg acgggctcat cagtatccag
      301 gccggtctcg ctcgccacct cccaagccac gccctcgagc ttccccctcg cggctgccgt
      361 catcgcaatc cgaaccgcag cgctctcgcg gccggccttg gtccagggat gatcgccgat
      421 ggcgaagatc aggctgatcg gcggcttgcc gttcaggtgg cgctccatga ccttgcgctg
      481 gaacacctgg gtgattgaat tggtggtcac gaaaccgaag cggcgcagga tcgacttctt
      541 ccgagccagg atctcggccg accgatccca ccagtacatg acgaaatcgg cgctatcgtt
      601 catgcccttg tgggcggccc aaagggcctc ggcatagccg ggcggcatcc gcgagcgcag
      661 cgtcgagccg ccaataaacg gcggattccc cacgatgaac tccgcctccg gccactccgg
      721 ccgccgggcg ttgggatagg tctcgacccg cacgccgtcc ttttcgacca cggtggtcag
      781 gggatagccg tcccaggtca gcaccgcgtc gcggttttcg atattgtgga aggccttcag
      841 gatcggttcg gccgggtggc cgctgcggct gcgataatgc tgttgcagat agcccagcca
      901 cagcaccagt tcggcgatgg cggcggcgcg gggattgagc tcaatgccca ggaactggtg
      961 cggatccacc gtctcgaagc ccatgctttc ggcctcgccc agctggacca gggtttccag
     1021 aacctcggcc tccagggtct tcatcagctc cagcgacaca tagaggaaat tggccgtgcc
     1081 gcaggccggg tccagcacgc gggtggcgca cagctgatgg tgaaaggcgc gcacggcctc
     1141 gcgggcggcc ttttcgtcgc cggcgtcggc cagggcctcg gccttggtgc gcaccgcccg
     1201 ccagtcggcg cgcaggggct ccatcaccgt cacattgacc agtcgctcga cataggcgcg
     1261 cggggtgtag tgggcgccca ggcggcggcg ctcattgggg tccagcgcct gttcgaccag
     1321 ggtgccgaag atggcgggtt caacctcgcg ccagttgtgc gaggcggcga ccagcagttc
     1381 gccgatctct tcgcggccca gttcaaacgc cgtggtctgt ttgaacaggc cgccgttgaa
     1441 gtgcagcacg cgggcatcga tggccatgga ctcgccgccg gtgtccatcg ccgtccacag
     1501 ctccttgacc cgctggggaa agcgctcggg cgtctcgacg ctcttgcgca gcagttgcgt
     1561 aaacgcgccc ttgggcagca gttccacgtc ctcggcgaac atcgtgaaga tgcagcgcat
     1621 caggaacagg gccacgtctt cggcccgcgc gcccttggct tccagggcct tggagacggc
     1681 ggccaggcgt tcggcgatct ggcgggtgac gcgggccgac tcgcgcgtcg ggtccaggct
     1741 gtgcggatcg gtccagatcg tggccaggcg ggcgcggagc ctgggatcgc gcagctcgtc
     1801 cagatagatc cggaaaccct tgcggtctgg gtaataggca tagccccggc cggtgccgga
     1861 aaagtcggca tagatctcga aacagtggcc cacgtcggcg acgatcagga acggcggggc
     1921 ggcatggtcg gtgggcagca ggtgcacata gtcattggcc tggttgaagg ccttgcgcat
     1981 cagcacgtcc cagcccttgg ccgtggagcg gcggcccagc tgctcgggct ctgcatcggc
     2041 tcccggcagc gacagttggt cgggcgcggc cttcttggcg ccgggggcgt gggtctgctt
     2101 ggcctccagc acgaaggcgg cgcgcttgta gaggtcgatg cgcttggcgc tggccccgtc
     2161 cagggtggtg gagctgacgg cgcgctcgaa cacatagtcg ttcagatcac gggccgcgcc
     2221 ggccggatcg gggcgcggca cgcccagcac gtcgcacagc tcggacagaa acatctggta
     2281 attggcgcgc tcggcgccgc cctcgcgcgc ggtccagcgc gtgatgaagt cttcgacgtt
     2341 cataagcccc gatcacatcc ccaacagctc tgcaaccata ggccgctttg ttcgggcttg
     2401 gcgagacggt ccc
//
LOCUS       DAOMEO010000461         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4693_length_2410_cov_2.598726, whole genome shotgun sequence.
ACCESSION   DAOMEO010000461 DAOMEO010000000
VERSION     DAOMEO010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4693_length_2410_cov_2.598726"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLH31_10665"
     CDS             <1..1103
                     /locus_tag="PLH31_10665"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQR89698.1"
                     /translation="MAGHAIQHAVSRAGLDGGEVEDVALGCGGPEGATGMNVARNAAM
                     WAGLPVTVSGQTINRFCSSGLQAIATAANYVRNDGANVAIGGGVESISLVQGGGHMNR
                     HHITEEKLMKSHPALWMAMIDTADIVAKRYNVSREYQDEYALRSQQRIAAAQAAGLFK
                     DEIVPMATKMKVVNKETKEESLVDYVVDKDECNRPETTLEGLSSLKPVMGEGKFVTAG
                     NASQLSDGAAAVVIMDAKEASRRGLEPMGLFKGFAVAGCEPDEMGIGPVYAVPRLLER
                     HGLKVDDIDLWELNEAFASQCLYSRDKLGIDPEKYNVNGGSIAIGHPFGMTGARCAGH
                     LLMEGKRRGAKLGVVTMCIGGGMGAAGLFEIF"
     gene            1299..1433
                     /locus_tag="PLH31_10670"
     CDS             1299..1433
                     /locus_tag="PLH31_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89699.1"
                     /translation="MAIKAFFALALVLSVTIGLLLPTPGAHKPRPAQSSIETIELRRG
                     "
     gene            complement(1430..1882)
                     /locus_tag="PLH31_10675"
     CDS             complement(1430..1882)
                     /locus_tag="PLH31_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89700.1"
                     /translation="MLRTAALWILVLSLTACGEAPMGASEEAAPPADAPASAKGANYS
                     GDIDVVGTEPFWAVKIRSGSVALTRPDSPEVSNANPGVRIDGEQGVWDSSGVDEEKGR
                     LVVRLTPGVCTDGMSDRVYRFYAEVWIDGETLKGCAEHTAVLAAQPKP"
     gene            1929..>2410
                     /locus_tag="PLH31_10680"
     CDS             1929..>2410
                     /locus_tag="PLH31_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HQR89701.1"
                     /translation="MTRKASPAFLEFFAGGGMARVGLGEGWTCAFANDFDPLKAAAYR
                     ANFADAPEHFHQEDVWTIEAESLPTADLAWASSPCQDFSLAGARAGLTGGRSSAFFGF
                     WKLMQALNAKGRSPDTIVIENVVGLLTSHDSRDFAALCQALSDEGYRFGALEVDAARF
                     "
BASE COUNT          419 a          832 c          765 g          394 t
ORIGIN      
        1 ccatggccgg tcacgccatc cagcacgccg tgtcgcgcgc cggcctcgac ggcggcgaag
       61 tggaagacgt ggccctgggc tgcggcggtc ctgaaggcgc caccggcatg aacgtggccc
      121 gcaacgccgc catgtgggcc ggcctgccgg tcaccgtttc cggccagacc atcaaccgct
      181 tctgctcctc gggcctgcag gccatcgcca cggcggccaa ctatgtgcgc aacgacggcg
      241 ccaatgtcgc tattggcggc ggcgtcgagt cgatctcgct ggtgcagggc ggcggtcaca
      301 tgaaccgcca ccacatcacc gaagaaaagc tgatgaagag ccatccggcc ctgtggatgg
      361 cgatgatcga caccgccgac atcgtggcca agcgctataa tgtcagccgc gaataccagg
      421 acgaatacgc cctgcgcagc cagcagcgca tcgccgccgc ccaggccgcc ggcctgttca
      481 aggacgagat cgtcccaatg gccaccaaga tgaaggtggt caacaaggaa accaaggaag
      541 agagcctcgt cgactacgtg gtcgacaagg acgagtgcaa tcgccccgag actaccctgg
      601 aaggcctgtc cagcctcaag cccgtcatgg gcgaaggcaa gttcgtcacg gccggcaacg
      661 cgagccagct gtcggacggc gccgccgccg tggtgatcat ggacgcaaag gaagcttcgc
      721 gtcgcggcct ggagccgatg ggcctgttca agggcttcgc cgtcgccggc tgcgagcctg
      781 acgaaatggg catcggcccc gtctatgccg tgccgcgcct gctggaacgc cacggcctga
      841 aggtcgacga catcgacctg tgggaactga acgaagcctt cgccagccag tgcctctaca
      901 gccgcgacaa gctgggcatc gatccggaaa agtacaacgt caacggcggc tccatcgcca
      961 tcggtcaccc cttcggcatg accggcgccc gctgcgccgg ccacctgttg atggaaggca
     1021 agcgtcgcgg cgccaagctg ggcgtggtga ccatgtgcat cggcggcggc atgggcgcgg
     1081 cgggtctgtt cgaaatcttc tgacttcgac ccagatcaga gcaccaaaag ccaagtcggg
     1141 ccggagacct tcaagagggt ttccggcccg ttttgcattc tggcggggct ttccggagat
     1201 tttttcgggg agcgcttgaa ccccggtctt tggcaaccat ttgtgttgct gaaggcgcat
     1261 cccgcgatgt ccgcgcgacg ccagatcaga gatccgccat ggccatcaag gccttcttcg
     1321 ccctcgccct cgtgctctcc gtgacgatcg gcctgctgct gcccacgccc ggcgcgcaca
     1381 agccgcgccc ggcccagtca tcgatcgaga cgatcgagct gcggcgcggt tagggcttgg
     1441 gctgcgcagc cagcaccgct gtgtgctcgg cgcagccttt cagcgtttcg ccgtcgatcc
     1501 acacctcggc atagaaccgg tacacccggt ccgacatgcc atcggtgcag acgcccggcg
     1561 tcaggcgaac gaccagccgg cctttctctt catcaacgcc gctggagtcc cacacgccct
     1621 gctcgccatc gatccgcacg cccggattgg cgttgctgac ctcgggactg tcggggcggg
     1681 tcagggccac gctgccggag cggatcttca cggcccagaa cggctcggtt ccgaccacat
     1741 caatatcgcc ggaatagttg gcccctttag ccgaggccgg cgcgtcagcc ggcggcgcgg
     1801 cctcttccga tgcccccatc ggcgcctcgc cgcaggcggt gagggacaga accaggatcc
     1861 acaaggcggc ggtgcgtagc atggcggcga ctgttccctg ttgcctgacg aggctagtct
     1921 cacacggcat gactcgaaaa gcctctcccg cgttccttga attcttcgcc ggcggcggca
     1981 tggcccgcgt ggggctggga gagggctgga cctgcgcctt cgccaacgat ttcgatccgt
     2041 tgaaggccgc agcctaccgg gccaatttcg ccgacgctcc ggaacacttc caccaggaag
     2101 acgtctggac catcgaggcc gagagcctgc cgaccgctga cctggcctgg gcctcaagcc
     2161 cttgccagga cttcagcctg gccggcgccc gcgctggtct gaccggcggc cgctcctcgg
     2221 ccttcttcgg cttctggaag ctgatgcagg ctctgaacgc caaagggcga agcccggaca
     2281 ccatcgtcat cgagaacgtg gtcggcctgc tgacctcgca cgacagccgc gacttcgccg
     2341 ccctgtgcca ggccctgtcc gacgagggct atcgcttcgg cgcgctcgag gtcgacgcag
     2401 cgcggttcat
//
LOCUS       DAOMEO010000462         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4695_length_2410_cov_2.078556, whole genome shotgun sequence.
ACCESSION   DAOMEO010000462 DAOMEO010000000
VERSION     DAOMEO010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4695_length_2410_cov_2.078556"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLH31_10685"
     CDS             <1..295
                     /locus_tag="PLH31_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR89702.1"
                     /translation="GNLKTIGGPAHIVDPGTNAKLHVSYRVFGIIRIPRDYWVLERAE
                     DYSWFISADPSFHDLWIYTRNPRPSQAEIAPLVARARALGYDTSLLEFPATGD"
     assembly_gap    385..394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            450..713
                     /gene="fliQ"
                     /locus_tag="PLH31_10690"
     CDS             450..713
                     /gene="fliQ"
                     /locus_tag="PLH31_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HQR89703.1"
                     /translation="MTGAEVLDVGRDAIWLTLQLCAPVLIVGLVVGVAIGLFQALTQI
                     QEATLVYAPKIVAIFISLLIFLPLMGALMSGFMRQIAARIAGM"
     gene            730..1485
                     /gene="fliR"
                     /locus_tag="PLH31_10695"
     CDS             730..1485
                     /gene="fliR"
                     /locus_tag="PLH31_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HQR89704.1"
                     /translation="MDSYATAAQVYAAGLVFARVGAMVMLMPGIGDTSVPARVRLGFA
                     LLMSLLLTPLLQNTVPGVPTSVGGLWGQVIHEILIGLMIGSVLKLFFTAMSTAGEVIS
                     MQTTLSFAQTTNPSMGQSSTAVATFLSMLGLTLIMATDLHHLFIGAIVKSYDLFPFSR
                     AVPVNDAATLAVRTVADSFSLGIQLAAPVIVFSLVFNLATGLVGRVMPSFQIFFVASP
                     LSVILGLSLLALSLGGIAMVWTDRYRDLLMIFN"
     gene            1495..>2410
                     /gene="flhB"
                     /locus_tag="PLH31_10700"
     CDS             1495..>2410
                     /gene="flhB"
                     /locus_tag="PLH31_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HQR89705.1"
                     /translation="MAEDTDNDSKTEEPSAKKLSDARAKGDVAKSADVPQLASLVGAV
                     AVILMAGGWLTRDLSAALTPFLAHAGRMDLSSGGAVVIARQAIMAALPPLVMVMVTTA
                     VFGFIAHVAQTGFMMTPEKIKPDLKKLDIIKGFGRLFGIDGLVQFAKSALKFIVTGII
                     AWFVLEPHADEIVNMVGMAPAEMIPVSMRLATKLFMAVILLLTLTAGFDWFWQRQRFM
                     KRMRMTLQEVKDEYKQSDGDPHIKAKRRQIQMQRSRQRMMQAVPKATVVVMNPTHYAV
                     ALKYEPGETPAPMCVAKGMDDLALKIRAV"
BASE COUNT          375 a          797 c          763 g          465 t
ORIGIN      
        1 gggcaatctc aagacgatcg gcggcccggc gcatattgtt gatccgggca cgaacgccaa
       61 gctccacgtc agctaccgcg tcttcggcat tatccgcatt ccgcgcgact attgggtgct
      121 ggagcgggcg gaggactaca gctggttcat ctcggccgac cccagcttcc atgacctgtg
      181 gatctacacg cgtaatccgc gccccagtca ggccgagatc gctccgctgg tcgcgcgcgc
      241 cagggcgctg ggctatgaca ccagcctgct ggaattcccc gcgacgggag actaggcgtc
      301 cccctgcggc caggggcttt ctgtgggggc acgacattgc tgaggcagag cgccgcagcc
      361 accccctgga cctgcccagg caacnnnnnn nnnnatcttg aaacctcgat tctgagccct
      421 gttcgggcct gctctcacga ggcttttgga tgaccggcgc cgaagttctt gatgtcggtc
      481 gcgatgcgat ctggctgacc cttcagctgt gcgcgcccgt gctgatcgtc ggcctggtgg
      541 tcggcgtcgc catcggcctg ttccaggccc tgacccagat ccaggaagcc accctggtct
      601 atgcccccaa gatcgttgcg atcttcattt cgctgctgat cttcctgccg ctgatgggcg
      661 cgctgatgtc cggtttcatg cgccagatcg ccgcccgcat cgcgggcatg taggggaggc
      721 gaggggcgtt tggactccta cgcgacagcc gcccaggtct atgccgccgg cctcgtgttc
      781 gcgcgggtcg gggccatggt catgctgatg cccggcatcg gcgacacctc ggtgccggcc
      841 cgcgttcgcc tgggctttgc tctgctgatg tccctgttgc tgacgccgct gttacagaac
      901 accgtccccg gcgtgcccac ctcggtcggc ggcctgtggg gccaggtgat ccacgagatc
      961 ctgatcggcc tgatgatcgg ctcggtgctg aagctgtttt tcaccgccat gtcgacggcc
     1021 ggcgaggtga tctcgatgca gaccaccctg tcattcgccc agactaccaa cccgtcgatg
     1081 ggccagtcca gcacggccgt ggcgaccttc ctgtcgatgc tgggcctgac cctgatcatg
     1141 gccaccgacc tgcaccatct gttcatcggg gccatcgtca agtcgtacga cctgttcccg
     1201 ttcagccgcg ccgtgccggt gaacgacgcc gccaccctgg cggtgcgcac agtggccgac
     1261 agcttttcgc tgggcatcca gctggccgct ccggtgatcg tgttctcgct ggtgttcaac
     1321 ctggcgacag gtctggtggg acgggtcatg ccgtcgttcc agatcttctt tgtggcgtct
     1381 ccgctcagcg tgatcctggg cctgtctctg ctggccctca gccttggcgg catcgccatg
     1441 gtctggaccg accgctaccg cgacctcctg atgatcttca actaggggcg ggccgtggcg
     1501 gaagacacag acaacgactc gaagacagaa gaaccctcag ccaagaagct gtcggacgcc
     1561 cgcgccaagg gcgacgtcgc caagagcgcc gatgttcccc aactggcctc gctggtgggc
     1621 gcggtggcgg tgatcctgat ggcgggcggc tggctgaccc gtgacctgtc ggcggcgctg
     1681 accccgttcc tggcccatgc cggtcgcatg gacctgagca gcggcggggc ggtggtcatc
     1741 gcccgtcagg ccatcatggc ggcccttccg ccgctggtca tggtgatggt gaccacggcc
     1801 gtgttcggct tcattgccca cgtggcccag accggcttca tgatgacgcc ggagaagatc
     1861 aagccggacc tcaagaagct ggacatcatc aagggctttg gccggctttt cggcatcgac
     1921 ggcctggtgc agttcgccaa gtcggcgctg aaattcatcg tcaccggcat cattgcctgg
     1981 ttcgtgctcg agccgcacgc cgatgagatc gtcaacatgg tcgggatggc gccggccgag
     2041 atgatccccg tgtcgatgag gctggcgacc aaacttttca tggccgttat cctgctcctg
     2101 accctgacgg cgggcttcga ctggttctgg cagcgtcagc gcttcatgaa gcggatgcgc
     2161 atgacccttc aggaggtcaa ggacgagtac aagcagtcgg acggcgaccc acacatcaag
     2221 gccaagcgcc gccagatcca gatgcagcgc tcgcgtcagc ggatgatgca ggcggtgccc
     2281 aaggccaccg tcgtggtcat gaacccgacc cactacgccg ttgcgctgaa gtacgagccc
     2341 ggcgagaccc ccgcgccgat gtgcgtggcc aagggcatgg acgacctggc cctgaagatc
     2401 cgcgccgtcg
//
LOCUS       DAOMEO010000463         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4714_length_2399_cov_2.992747, whole genome shotgun sequence.
ACCESSION   DAOMEO010000463 DAOMEO010000000
VERSION     DAOMEO010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4714_length_2399_cov_2.992747"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLH31_10705"
     CDS             complement(<1..1197)
                     /locus_tag="PLH31_10705"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQR89706.1"
                     /translation="MTKIACLAGVAVAALLAASAAAQTSEEATKAYLDQIQARNDILH
                     AVIAVNPAALTDAKALDAERQAGKVRSAIHGLPILLKDNIEANDGLATTAGSLALKNN
                     VTGRDAPLVKGLRDAGAVLLGKANLSEWANIRSSKSISGWSAVGGTVRNPYVLDRSAC
                     GSSSGLGAAVAAGLAWAAIGTETDGSITCPAAINGLVGLKPTVGLVSRTHIIPISHSQ
                     DTAGPMTTSVKDAADILTAIAGSDPADVATKAADSHKTDYAKGLDKNALKGVTLAVAR
                     FYTGYSPKTDLVFEQALADLKAQGATLVEVKEFDEGPIGKAEGVVLYTELKVDLAAYL
                     ASTDPAKVPSRTLADLIAFNKATPHEFEWFGQESFEKAEKTKGYSDPDYIKAAADAKR
                     LAGPEGI"
     gene            complement(1292..2074)
                     /locus_tag="PLH31_10710"
     CDS             complement(1292..2074)
                     /locus_tag="PLH31_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR89707.1"
                     /translation="MTTYTDLIASITETKTGHAAHVTDDWKQGRTTYGGLSAALCLEA
                     AVRTLPQAPPLRSGQFAFIGPAAGELAIETTILRQGKSTLFVGVDLVGEQGLATRAIL
                     TFGAARESLVSHSDLPCPAVLKPDDCEPFFPKGGGPGFGGQFEVRIAGGARPLSGQPP
                     EYLLWIRHRDPAARSVAALVALADTPPPPAMTLFPKFGPISTMTWSLDIVGLPAEDDE
                     GWRLLRSTAETIGDGYSTQGMMLWDAAGRPLIAARQNVAVFV"
BASE COUNT          353 a          780 c          856 g          410 t
ORIGIN      
        1 gatgccctcg gggccggcca gacgcttggc gtccgcagca gccttgatgt agtcggggtc
       61 gctgtagccc ttggtctttt cggccttctc gaagctctcc tggccaaacc actcgaactc
      121 gtgcggcgtg gccttgttga aggcgatcag gtcggccagc gtgcggctgg gcaccttggc
      181 cggatcggtc gaggccagat aggcggccag gtcgaccttc agctcggtat agagcaccac
      241 gccctcggcc ttgccgatcg ggccttcgtc gaactctttc acctcgacca gggtcgcgcc
      301 ctgggccttc aggtcggcca gggcctgttc gaacaccaga tcggtcttgg gcgaatagcc
      361 ggtatagaac cgcgccacgg cgagggtgac gcccttcagg gcgttcttgt cgaggccctt
      421 ggcatagtcg gttttgtggc tgtcggccgc cttggtggcg acatcggcgg gatcggagcc
      481 ggcgatggcg gtcaggatgt cggcggcgtc cttgaccgag gtggtcatcg ggccggccgt
      541 gtcctggctg tggctgatgg gaatgatgtg ggtgcgcgag accaggccca cggtgggctt
      601 caggccgacc agaccgttga tcgccgctgg gcaggtgatc gagccgtcgg tctcggtgcc
      661 gatggcggcc caggccagac ccgccgccac ggccgcgccc aaaccgctgg acgagccgca
      721 ggcgctgcgg tccagcacat agggattgcg caccgtgccg cccaccgcgc tccagccgct
      781 gatcgacttg ctggagcgga tattggccca ttccgacagg ttggccttgc ccagcagcac
      841 ggctccggcg tcgcgcagac ccttgaccag cggcgcgtcg cggccggtca cgttgttctt
      901 cagggccaga gatccggcgg tggtggccag accgtcattg gcctcgatat tgtctttcag
      961 caggatgggc aggccgtgga tggccgagcg caccttgccg gcctggcgtt cggcgtcgag
     1021 ggccttggcg tcggtcaggg cggcggggtt cacggcgatc acggcgtgca ggatgtcgtt
     1081 cctggcctgg atctggtcga gataggcctt ggtcgcctct tcagaggtct gggcggcggc
     1141 gctggcggcc agcagggcgg cgacggccac tccggccagg caggcgatct tggtcatgcg
     1201 aattccccta cgcgggccga gacgacaggt cggccgaacg ggccggagga tgacggcaaa
     1261 acctcgccgc cgccaagccc ttcacacgcg ctcaaacgaa aacggccacg ttctgccgcg
     1321 ccgcgatcaa cgggcgaccg gccgcgtccc acagcatcat gccttgcgtg gaatagccgt
     1381 cgccgatcgt ctcggcggtg ctgcgcagca acctccagcc ctcgtcgtct tccgcgggca
     1441 ggcccacgat gtcgagcgac caggtcatgg tcgagatcgg gccgaacttc ggaaacagcg
     1501 tcatggccgg cggcggcggg gtgtcggcca gggccacaag ggccgccacg gagcgcgcgg
     1561 ccgggtcgcg gtgccggatc cacagcagat attccggcgg ttgacccgac agtggtcgcg
     1621 cgccgccggc gatccgcacc tcgaactgcc cgccaaagcc ggggccgccg cccttgggga
     1681 agaacggctc gcagtcgtcg ggtttgagca cggccgggca gggcaggtcg ctgtgcgaca
     1741 ccagcgattc gcgcgccgcg ccaaaggtca ggatcgcccg cgtcgccagg ccctgctcgc
     1801 ccaccagatc gacgccgacg aacagggtcg acttgccctg gcgcagtatg gtggtttcga
     1861 tggccagctc tccggccgcc gggccgatga aggcgaactg gcccgagcgc aggggcgggg
     1921 cctgcggcag ggtccgcacg gcggcttcca ggcacagcgc cgccgacagc ccgccatagg
     1981 tggtgcggcc ttgcttccag tcatctgtca catgggcggc gtggccggtc ttggtttcag
     2041 tgatgctggc gatgaggtcg gtataggtag tcacgcacga gtcccttggg gttttgtcga
     2101 aaggttcgtt ttctttcgct acggaagtca agggtgagtt gatggccacc gccgggacgc
     2161 cccctccgtc acgtcgcgta tccgcgccgc tgacttagat cctcccgctt gcgggggagg
     2221 tggcgcgaag cgccggaggg gtaagtcctt ctgttcatgg cagcccctct ccgtcggctg
     2281 cgccgacacc tctcccacag ggagaggatc ttgagacacg gcgtctcagc ctcacgcggc
     2341 atcagccact gaaggggttg gggaggcgcg aagcggccgg ggcgaacccg gatgaccgt
//
LOCUS       DAOMEO010000464         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4717_length_2399_cov_1.910836, whole genome shotgun sequence.
ACCESSION   DAOMEO010000464 DAOMEO010000000
VERSION     DAOMEO010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4717_length_2399_cov_1.910836"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1434)
                     /locus_tag="PLH31_10715"
     CDS             complement(199..1434)
                     /locus_tag="PLH31_10715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQR89708.1"
                     /translation="MRTAMTCSDTALWNDPDIRSAPGAFRTALRVAAENWAVGSLSFI
                     LPSGKRFDIHGKTPGPSAVIQVRDYRFVRRALASGDIGFAEGFIAGEWDTPDLSTVLE
                     AFSLNFDRLHDLVEGNVVMRTINALYHLFNRNDRRGSKKNIHAHYDLGNAFYSRWLDP
                     GMTYSSALYQTPDQPLEQAQTAKYAALARTIDLQADQTVLEIGCGWGGFAEFAAKEVG
                     ARVTGITISQAQHDFARKRLFEMGLADKADIQLIDYRDVQGRFDRVASIEMFEAVGEE
                     YWPTYFGKIHEVLTPGGRAGLQIITIRDELFEHYRTHTDFIQRYIFPGGMLPSETRLK
                     AETTRAGLEWRDISHFGQSYADTLAEWGRRFEAAWDDIATLKFDERFRRLWRFYLSYC
                     EAGFRTERTNVIQLGLSQA"
     gene            complement(1484..>2399)
                     /locus_tag="PLH31_10720"
     CDS             complement(1484..>2399)
                     /locus_tag="PLH31_10720"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQR89709.1"
                     /translation="ALPAPAKQPASDGLGDWNLHPTKPDWSKGFSSWTPGEAGAAERL
                     QAFLASQVQNYDKRDIPGVEVTSRLSPHLHFGEIGPRQVWAATRSAIAAGDVPAGQGE
                     KFLSEVGWREFNHSILFHRPDITTANFRPEFDAFPWNRDDAAFKAWTRGQTGYPIVDA
                     GMRELWTTGFMHNRVRMIVASFLIKHLLIDWRQGEAWFWDTLLDADLPNNVGNWQWVA
                     GSGADAAPYFRIFNPISQGEKFDPNGVYVRRWVPELSNLPDKLIHSPWTAAPHLTPAA
                     KRTYLRPIVDHSQARERALAAYQSLKGG"
BASE COUNT          431 a          751 c          781 g          436 t
ORIGIN      
        1 cctcgcccag cagctgttct tcctgaagtt cgttgcacca ggccgtgacc gcatcgacgg
       61 ccgtcgatgc gtcgaactgg gtgatgtagg tccccccgtg gaaatccagg accatggtgt
      121 agaggttcat gggacgcgac ctcgatgtgc tttcgactat ctacgcacac ccaaggtcgc
      181 gacgcgaagg cgaacttgct aggcctggct cagcccaagc tggatcacat tggtccgctc
      241 ggtgcggaag ccggcttcgc aatagctgag atagaaccgc caaagccggc ggaagcgttc
      301 gtcgaatttc agcgtggcga tatcgtccca tgccgcctcg aagcgccggc cccactcggc
      361 cagggtgtca gcatagctct ggccgaaatg gctgatatcc cgccattcca gcccggcccg
      421 ggtggtctcg gccttcaggc gcgtctcgct gggcaacatg ccgcccggga agatgtagcg
      481 ctggatgaag tcggtatggg tccgatagtg ctcgaacagc tcgtcgcgga tggtgatgat
      541 ctgcaggccg gcgcggcccc cgggggtcag cacctcatgg atcttgccaa aataggtggg
      601 ccagtactcc tcgcccaccg cctcgaacat ctcgatggag gcgacgcggt caaagcggcc
      661 ttgcacgtcg cgatagtcga tcagctggat atcggccttg tcggccagac ccatctcgaa
      721 caggcgcttg cgggcaaagt cgtgctgggc ctgcgagatg gtgatgccag tgaccctcgc
      781 ccccacctcc tttgcggcga actcggcaaa gccgccccag ccgcagccga tctccagaac
      841 ggtctggtcg gcttgcagat cgatggtccg ggccagggcc gcatatttgg ccgtctgggc
      901 ctgctccagc ggctggtctg gcgtctgata cagggctgac gagtaagtca tgccggggtc
      961 cagccagcgc gaatagaagg cgttgcccag gtcgtagtgg gcgtggatat tcttcttcga
     1021 tccccgccga tcgttgcggt tgaacaggtg atagagagcg ttgatcgtcc gcatgacgac
     1081 attgccctcg accagatcgt gcaggcggtc gaagttcagt gagaaggctt ccagcaccgt
     1141 cgacaggtcg ggcgtgtccc actcaccggc aatgaagcct tcagcaaagc cgatgtcgcc
     1201 cgaggccagg gcccgccgca cgaagcgata gtcgcgtacc tggatcacgg cgctggggcc
     1261 gggcgtcttg ccatgaatgt cgaaacgctt gcccgacggc agaatgaagc tgagggagcc
     1321 gaccgcccag ttttcggccg ccacgcgcag agccgtccga aacgcaccag gtgcgctgcg
     1381 gatgtcagga tcattccaga gcgcggtgtc cgaacaggtc atggcggttc gcatgaaatc
     1441 tcctggcatt aaacgctctc gggaccgtcc ccgcaaaccc tcctcaacct cccttgaggc
     1501 tttgataggc agccagcgcg cgttcccgcg cctggctgtg atcaacgatc ggtcgcaagt
     1561 atgtacgctt ggcggccggc gtcagatgcg gggccgccgt ccacgggctg tggataagct
     1621 tgtcgggcag attggacagc tccggaaccc agcggcgaac atagacgccg ttggggtcga
     1681 acttctcgcc ctggctgatc ggattgaaga tccggaaata gggggcggcg tcggcgccgc
     1741 tgcccgccac ccactgccag ttgcccacat tgttgggcag gtcggcgtcc agcagcgtgt
     1801 cccagaacca ggcttcgccc tgtcgccagt cgatcagcag gtgcttgatc aggaatgaag
     1861 cgacgatcat ccgtacacgg ttgtgcatga agccggtcgt ccacagctcg cgcatcccgg
     1921 cgtcgacgat gggatagccg gtctggcccc gggtccaggc cttgaacgcc gcatcgtcac
     1981 ggttccacgg aaaggcgtcg aactcggggc ggaaattggc ggtggtgatg tcgggacggt
     2041 ggaagaggat cgagtggttg aattcccgcc agcccacctc cgacaggaac ttctcgccct
     2101 ggcccgccgg cacgtcgcca gcggcgatgg cgctacgcgt cgcggcccag acctgacgtg
     2161 gcccgatctc gccaaaatgc aggtgcggcg acaggcggga ggtcacctcc acgccgggaa
     2221 tgtcgcgctt gtcatagttc tggacctgcg aggcaaggaa ggcctgcagg cgctcggcgg
     2281 cgcccgcctc gccgggcgtc cagcttgaaa agcctttcga ccagtccggc ttcgttggat
     2341 gcaaattcca gtcgcccagg ccgtcgctgg cgggctgttt ggctggagcc ggcaaggct
//
LOCUS       DAOMEO010000465         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4721_length_2396_cov_2.902606, whole genome shotgun sequence.
ACCESSION   DAOMEO010000465 DAOMEO010000000
VERSION     DAOMEO010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4721_length_2396_cov_2.902606"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..176)
                     /locus_tag="PLH31_10725"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="PLH31_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(230..418)
                     /locus_tag="PLH31_10730"
     CDS             complement(230..418)
                     /locus_tag="PLH31_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89710.1"
                     /translation="MVLRTTGANWLTLMMRAERAQRGWEPFGSEWLLYGPTDQDVVQD
                     PDSGQEEDDAPPTLLAAE"
     gene            726..932
                     /locus_tag="PLH31_10735"
     CDS             726..932
                     /locus_tag="PLH31_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQR89711.1"
                     /translation="MATGTVKWFNGTKGFGFIQPDDGGSDVFVHISAVERAGLRTLNE
                     GQKITYELEQDKRSGKMSAGQLQA"
     gene            1062..2249
                     /locus_tag="PLH31_10740"
     CDS             1062..2249
                     /locus_tag="PLH31_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR89712.1"
                     /translation="MRSVRILIVLLAGLTLAACGSLPRDAFTASDIAGNAPHGLADVR
                     FNATDGQAGIAFAEAARSRMERQKTFDVLAISGGGANGAYGAGILTGWTQTGDRPEFD
                     IVTGVSTGALTAPFAFLGPDWDQRLKMAYTSPAAERILKRRGIDLLFRPGFYTTASLR
                     RLIETYVDQPMLYAVAVEHARGRRLLIATTNLDTEETVIWDMGAIASRGDAGALKLFQ
                     DVLVASSSIPGVFPPTLIEVQGKGRRLSEMHVDGSVTTPFFVAPEALLLWTTPEQGVA
                     RPGKVRVIVNGKVGGSFGFTRGGTLSIIGRSWETMTKALVRTHLTASAAFARRNGGSL
                     LFTAIPDRAEVDTSGLDFAAANREALFRLGRDSALDGTAWREPDAPGDPPVASTASVA
                     AVP"
BASE COUNT          388 a          850 c          735 g          423 t
ORIGIN      
        1 cctagggcca atcgacattc agccgaccat tgtcctttat tcaccgtcat tcccgccctt
       61 gtggcgggaa cccctggttc agctggcagg tgagacgcaa gcggaccgcc agcggtccgc
      121 ccctcccgca cctgcggcgg gcataggtgt tcccgccaca agggcgggaa tgacggagtt
      181 taagagatcg gccgccgtcg cgatcctgaa tgtcgatcca ctctaggccc tattcggccg
      241 ccaacagggt ggggggagcg tcgtcctctt cctggccgct atcggggtcc tggacaacgt
      301 cctgatcggt ggggccgtac aggagccatt cgctaccgaa gggctcccag cccctctgcg
      361 cccgttcggc gcgcatcatc agggtcagcc agttcgcacc ggtcgttcgc aaaaccatcc
      421 tcgcctcctg cggcctggcg ccatttttga gaatgactcg caatagctag agtcgcaagg
      481 ctgtttcgtc gggcctggct ggactgcgcc ggcctttccg acaggccgaa aaaccaggct
      541 ccaagattct ccttgctggc gacggcttat gcccccatca tggagctatc gaattcgttg
      601 ccgttcggcc tgctattcca tgcttccttc gaggaagcgc cggacgcgcc taccgatccc
      661 catcgaaatt tgatcgtcga acggaacgct tccggcggcg actactatcc aaaggactac
      721 acaccatggc taccggcacc gttaagtggt tcaacggcac caagggcttc ggcttcatcc
      781 aaccggatga cggcggctcg gacgtttttg tgcacatctc ggccgttgaa cgcgccggcc
      841 ttcgcaccct caatgagggc cagaagatca cctacgaact ggaacaagac aagcgcagcg
      901 gcaagatgtc cgctggccag ctgcaggctt aatccagtca agaacggcgc cggtacgccg
      961 gcgctcttct gcactcctat aggccggcgc ttgcgccggc ctatttgcgt ttggaggtct
     1021 ctgttctagt ctggaggcct tcaaccgtct tggtggtcag catgcgctcg gtccggatcc
     1081 tgatcgtcct gctggccggg ctgaccctgg ccgcctgcgg ctccctgccg cgcgacgcct
     1141 tcaccgccag cgacatcgcg ggcaatgcgc cgcacggcct tgccgatgtg cgcttcaacg
     1201 ccacagacgg ccaggccggc atcgccttcg ccgaagccgc ccgcagccgc atggagcgcc
     1261 agaagacctt cgacgtgctg gccatttccg gcggcggcgc gaacggggcc tatggcgcgg
     1321 gcattctgac cggctggacc cagaccggtg accggcccga gttcgacatc gtcaccggcg
     1381 tcagcacggg cgccctgacc gcgcccttcg cctttctggg acccgactgg gaccagcgcc
     1441 tgaaaatggc ctataccagc ccggccgccg agcggatcct gaagcggcgg ggcatcgacc
     1501 tgctgtttcg ccccggcttc tacaccaccg catccctgcg tcgcctgatc gagacctatg
     1561 tcgaccagcc catgctctat gccgtggctg tcgagcacgc ccggggccga aggctgctga
     1621 tcgccaccac caacctggac accgaggaga cggtgatctg ggacatgggc gccatcgcct
     1681 cgcgaggcga cgccggcgcg ctgaaactgt tccaggacgt tctggtggcg tcgtccagta
     1741 ttccaggggt ctttccgccg accctgatcg aggttcaggg caagggccgg cggttgtcgg
     1801 aaatgcatgt ggacggcagc gtcaccaccc cgttcttcgt ggcccccgaa gccctgctgc
     1861 tatggacgac ccccgaacag ggcgtagccc ggccgggcaa ggtgcgggtg atcgtcaatg
     1921 gcaaggtcgg cggcagtttc ggctttaccc gtggcggcac cctgtcgatc atcggccggt
     1981 cgtgggaaac catgaccaag gccctggtcc gcacccatct gaccgccagc gccgcctttg
     2041 cgcgtcgcaa tggcggcagc ctgctgttca cagccatacc cgaccgcgcc gaagtcgaca
     2101 cctcgggcct ggatttcgcc gccgccaacc gcgaggcgct gtttcggctg ggtcgcgaca
     2161 gcgccctcga cgggacggcc tggcgcgagc cggacgcgcc gggcgacccg cccgtcgcct
     2221 ccaccgcctc ggtcgccgcc gtcccctgac gtctagcttc ggcgcgcagg ttcagcgccg
     2281 agcgcacggc gcaacaggcc ggctcagacg gcgtagctcg cgccaaccgc ctcggccacc
     2341 ttgatgccgt ccaccgccgc cgacaggatg ccgccggcat agcccgcgcc ttcgcc
//
LOCUS       DAOMEO010000466         2396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4722_length_2396_cov_2.238787, whole genome shotgun sequence.
ACCESSION   DAOMEO010000466 DAOMEO010000000
VERSION     DAOMEO010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4722_length_2396_cov_2.238787"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>796)
                     /locus_tag="PLH31_10745"
     CDS             complement(<1..>796)
                     /locus_tag="PLH31_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQR89713.1"
                     /translation="GVYHMLAKADFVGGFQGEGRGQISMLPRLRPNRFYDLVIEVAIV
                     RPGPIQGDMVHPYLRRRNGQEPVEWPAPSPEHGPPDELYGILGNTYGVPLFQEQAMSL
                     AIEAAKFTPDEADGLRKAMATFKNLGTPAEYRDKFVEGMTGRGYQRDFAERCFKQIEG
                     FGHYGFPESHAASFAKLVYVSAWLKWAWPDVFCAVLINSQPMGFYQPAQLVRDAREHG
                     VEVRPPDVMASDWDCTLEAIPKAPPTANVIPDEAKRRSGTDPERRAS"
     assembly_gap    797..806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<807..>2396)
                     /locus_tag="PLH31_10750"
     CDS             complement(<807..>2396)
                     /locus_tag="PLH31_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQR89714.1"
                     /translation="FADGTPSLLCYPSDREAFARLTRLLTVGQRRADKGGCELHWEDF
                     LQHAEGQLALIVPPARLDDVFTRDLDRMTSDLRGRVWLTASRAYAARDLQRLARLDQL
                     ARAAGAPMVATNDVLYHAPERRPLQDVMTCVREHCTIQEAGFRLEANAERHLKTPQEM
                     ARLFERWPRAVEHTMEIVARIGFDLGQLKEEYPDEPVPPGKTAIQHLTDLTWAGAAWR
                     YPKGVPDKVADQLREELRLIGKMDYPNYFLTVHDIVQEARAMGILCQGRGSAANSSVC
                     FCLGVTSIDPTEHRLLFTRFISENRGEPPDIDVDFEHERREEVMQYVYRRYGRDYAAI
                     CGTVIHYRPRSAIRDVGKALGLTEDVTSLLAATVWGSWGSGLPEEHIRKAGLDPEAPE
                     IARAVNLATELLGFPRHLSQHVGGFVLTKRRLDETVPIGNAAMKDRTFIEWDKDDIDS
                     LGLMKVDILALGMLTAIQRGFGMLRAEHGLDIHDLADVPVEVKGETRWWELSASPRHD
                     ENGLFLGFRGVASDVTAQRES"
BASE COUNT          387 a          804 c          793 g          402 t
ORIGIN      
        1 cggaagcgcg gcgctctgga tcggtcccgg atcttcgctt cgcttcgtcc gggatgacat
       61 tggcggttgg cggagccttc gggatggcct ccagcgtgca gtcccagtcg ctggccatca
      121 cgtctggcgg ccgcacctcg acgccatgct cgcgcgcatc ccgcaccagc tgggccggtt
      181 ggtagaaccc catgggctgg gagttgatca gcaccgcgca gaagacgtcg ggccacgccc
      241 atttcagcca ggccgagaca tagaccagct tggcgaaact ggccgcgtgg ctttccggaa
      301 aaccgtaatg gccaaagccc tcgatctgct tgaagcagcg ctcggcgaag tcgcgctggt
      361 agccgcgccc cgtcataccc tcgacgaact tgtcgcggta ttcggctggc gtgccgaggt
      421 tcttgaaggt agccatggcc ttgcgcagac catcggcctc atcgggcgtg aacttggcgg
      481 cctcgatggc caggctcatc gcctgttcct gaaacagcgg cacgccatag gtgttgccca
      541 ggatgccgta aagctcgtcg gggggcccat gctcgggcga cggggcgggc cactccaccg
      601 gttcctgacc gttacgtcgc cgcagatagg gatggaccat gtcgccctgg atcggcccgg
      661 gccggacgat cgccacctcg atgaccagat catagaaccg gttgggcctg agcctgggca
      721 gcatcgaaat ctggccccgg ccctcgccct ggaacccccc cacgaaatcg gccttggcca
      781 gcatgtggta gacgccnnnn nnnnnnccga ttcacgctgc gccgtgacgt ccgacgcaac
      841 cccgcgaaag cccagaaaaa gcccgttttc atcatggcgg ggcgaagcgg acaattccca
      901 ccagcgggtt tcgcccttca cctcgaccgg aacgtcagcc aggtcgtgga tgtccaggcc
      961 atgctcggcg cgcagcatgc caaagccgcg ctggatggcg gtcagcatgc ccagggccag
     1021 gatatcgacc ttcatcaggc ccaggctgtc gatatcgtcc ttgtcccact cgatgaaggt
     1081 gcggtccttc atggccgcgt tgccgatcgg cacagtctcg tccagccggc gcttggtcag
     1141 cacgaaaccg ccgacatgct gtgacaggtg gcgggggaac cccagcagct cggtggccag
     1201 gttgacagcg cgggcgatct ccggcgcctc gggatccagg cccgccttgc ggatatgctc
     1261 ttccggcagg cccgagcccc agctgcccca aaccgtcgcg gccaacaggc tggtgacatc
     1321 ctcggtcagg cccagagcct tgcccacatc gcggatggcg ctgcgcggcc ggtagtggat
     1381 caccgtgccg cagatggcgg catagtcgcg gccatagcgg cggtagacat attgcatcac
     1441 ctcctcgcgc cgctcgtgct cgaagtcgac gtcgatgtcc ggcggctcgc cccggttctc
     1501 agagatgaaa cgggtgaaca gcaaccggtg ctcggtgggg tcgatcgagg tcacgcccag
     1561 gcagaaacag accgaggaat tggccgccga acctcgtccc tggcagagaa tccccatggc
     1621 ccgggcctcc tgcacgatgt cgtgcacggt caggaaatag ttgggatagt ccatcttgcc
     1681 gatcagccgc agctcctcgc gcagctggtc ggcgaccttg tctggcacgc ctttcggata
     1741 gcgccaggcg gccccggccc aggtcaggtc ggtcaggtgc tgtatcgccg tcttgccggg
     1801 cggcacgggt tcgtcggggt actcttcctt cagctggccc aggtcgaagc cgatgcgcgc
     1861 gacgatctcc atcgtgtgtt cgacggcgcg cggccagcgc tcgaacaggc gggccatttc
     1921 ctgcggcgtc ttcaggtggc gctcggcatt ggcctccagc cggaagcccg cctcctggat
     1981 ggtgcaatgc tcgcgcacgc aggtcatgac gtcctgcagg ggccgtcgtt cgggcgcgtg
     2041 gtagagcaca tcattggtgg cgaccatcgg cgcgccggcc gcgcgggcca gttggtccag
     2101 tcgcgccagc cgctgcaggt cgcgggccgc ataggcccgg ctggccgtca gccagacccg
     2161 gccgcgcagg tcgctggtca tgcgatccag atcgcgcgtg aagacgtcgt ccagcctggc
     2221 cggcggcacg atcagggcca gctggccctc ggcatgctgc aggaaatcct cccagtgcag
     2281 ctcgcacccg cccttgtcgg cgcggcgctg gccgaccgtg agcaggcggg tcaggcgggc
     2341 gaaggcctca cggtcggacg gatagcagag caggctgggc gtcccgtcgg cgaaat
//
LOCUS       DAOMEO010000467         2386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4746_length_2386_cov_2.467181, whole genome shotgun sequence.
ACCESSION   DAOMEO010000467 DAOMEO010000000
VERSION     DAOMEO010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4746_length_2386_cov_2.467181"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLH31_10755"
     CDS             <1..664
                     /locus_tag="PLH31_10755"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HQR89715.1"
                     /translation="SSKTSDPPVSCGSATSARYLPARQPDHDRLRTWEVAGTAHADTY
                     VMGASPMDSGTASTEDLARALAPTCNLLGLTLPGEINAAPQHHYVMMAALSALNRWVT
                     TGEAPPAGTRLTIDGTKPARLTLDEVGNAVGGIRSPWMDVPTCKLSGLAIAKSRMGAL
                     FGSTGPLDPGSLSRLYPAGRADYLAKFRDALASAIAAGFILPADRDEIEALAAASYPD
                     AA"
     gene            complement(646..1251)
                     /locus_tag="PLH31_10760"
     CDS             complement(646..1251)
                     /locus_tag="PLH31_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR89716.1"
                     /translation="MSVLNNNANSVPQSPPRKDVVRHRAALLDAAARVFAAGGVDAPL
                     GEIARQAGVGRGTLYRHFPERIDLIVALLNRNLDDLETQAADLADQPDALLTLIRTMI
                     DQDIDVAPLLDSDLIMPATLLERFVAICRVPLEAARRAGVVRPDLDETDLLDLTVMAG
                     SALKRWGLDERRVRAPRIQALLLEGICCAPSSPRARHAASG"
     gene            1337..>2386
                     /locus_tag="PLH31_10765"
     CDS             1337..>2386
                     /locus_tag="PLH31_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQR89717.1"
                     /translation="MQDHFDYIVIGAGSAGCVLANRLSADPTVTVLLVESGPSDNSLF
                     VQMPRGIGVLLQPGNERLFTYEARQGGNRKTETWVKGRTLGGSSSVNGMVYIRGAPRD
                     FDGWEAEGCAGWGWDEMRRRFIELEDHELGPGPDRGVGGPLKITVPEFGDPMSEALIA
                     GAGELGAPRVRDINDRASVDDGGFGYQPVTIHNGSRFSAAKAFIHPIRKRANLTVVSE
                     THALRLLFAGTRVSGVRLRDKGGERDVMCGREILQLSGIGPRELLTSLGIEVVAEAPN
                     VGKNLIEHRYLSIQHRTRAPSQNEKLRGGNMLASLLTYLVAGKGPMTRGAYEGGGFIK
                     TDPALAHPDIQIGVGL"
BASE COUNT          426 a          784 c          801 g          375 t
ORIGIN      
        1 atcgtcgaaa acttcagacc cgccggtttc atgtgggtca gcaacatctg ccaggtatct
       61 tccggcccgt cagccggatc atgatcggct gaggacctgg gaggtcgcag gaaccgccca
      121 cgccgacacc tatgtcatgg gagcctcgcc gatggacagc ggaacggcct ccacggaaga
      181 cctggccagg gcgctggcgc ccacttgcaa tctcctaggc ttgaccctgc cgggcgagat
      241 caatgccgca ccccagcacc actatgtgat gatggccgcc ctgtcggcgc tgaaccggtg
      301 ggtgaccact ggcgaggccc cgcccgccgg aacacgactg acgatcgatg ggaccaagcc
      361 cgcgagactg acgctcgacg aagtgggcaa tgccgtcggc ggcatccgct cgccatggat
      421 ggacgtcccg acctgcaaac tctccggtct ggccatcgca aaaagccgga tgggggctct
      481 gttcggttcg accggaccgc tggatccggg cagcctgtcg cggctctatc cggcaggccg
      541 cgcggactat cttgcgaagt tccgggacgc tctggcctcc gcgatcgcgg cgggattcat
      601 cctgcccgca gatcgcgacg agatcgaagc tctagcagcg gcgagctatc ccgacgcggc
      661 atgacgcgca cgcggcgagc tcggtgcgca gcagatccct tcaagcaaaa gggcctggat
      721 gcgcggcgcc cgcacgcggc gttcgtccag tccccagcgc ttcagggccg acccggccat
      781 cacggtaagg tcgagaagat cggtctcgtc gaggtcgggc cggacgacgc ctgcccgacg
      841 cgcggcctcc aggggaaccc ggcagatcgc gacgaagcgc tccagcagcg tcgcgggcat
      901 gatcagatcg ctgtccagca acggcgcgac gtcgatgtcc tgatcgatca tcgtccggat
      961 cagggtcagc agagcgtccg gctggtcggc gagatctgcc gcttgcgttt ccaggtcgtc
     1021 gaggttgcgg ttcagcaagg cgacgatcag gtcgatccgc tcggggaaat gccgatagag
     1081 ggttccccgc cctactccgg cctgccgcgc gatctcgccc agcggggcat cgaccccgcc
     1141 cgcggcgaac acccgcgccg cggcgtccag cagagccgcc cggtgccgca cgacatcctt
     1201 gcggggcggc gactgcggaa cagaattcgc attattgttc aaaacggaca ttgatgtact
     1261 gttttaccga gtggatagtg ctcgcctgga accgggaccg acgcaagcgt cacggaccat
     1321 atcagggacg aaagccgtgc aggaccactt cgactacatc gtcatcggcg ctggctccgc
     1381 gggctgcgtg ctggccaacc gcctctcggc ggaccccacc gtcacggtgt tgctggtcga
     1441 aagcgggccg tcggacaaca gcctgttcgt ccagatgccg cgcggcatcg gcgtgctgct
     1501 ccagcccggc aacgagcgtc tgttcaccta tgaagcgcgc cagggcggca atcgcaaaac
     1561 cgagacctgg gtcaagggcc ggaccttggg cggatccagt tcggtcaacg gcatggtcta
     1621 tatccggggc gcgccgcgtg atttcgacgg ctgggaggcc gaaggctgcg ccggctgggg
     1681 ctgggacgag atgcgtcgcc gcttcatcga actggaagac catgaacttg gccccggtcc
     1741 agaccggggc gtgggcggcc ccctcaagat cacggtaccg gagttcggcg acccgatgag
     1801 cgaggccctg atcgcgggcg ccggcgaact gggcgcgccg cgtgtgcgcg atatcaacga
     1861 cagggcgtcg gtcgacgacg gcgggtttgg ctatcaaccg gtgaccattc acaacggctc
     1921 gcggttcagc gccgcgaagg ccttcatcca tccgatccgc aaacgcgcca atctgacggt
     1981 cgtcagcgag acgcacgcgc tgcgcctcct gtttgcggga acccgggtga gcggcgtgcg
     2041 actacgcgac aagggcggcg aacgcgacgt gatgtgcggc cgcgagatcc tgcaattgtc
     2101 ggggatcgga ccgcgcgaac tgctgacctc gctcggcatc gaggtcgtgg ccgaggcgcc
     2161 taatgtcggc aagaacctga tcgagcatcg ctacctgtcg atccagcacc ggacgcgcgc
     2221 gcccagccag aacgagaagc tgcgcggagg caatatgctt gcaagcctcc tgacctatct
     2281 ggtcgcgggt aaggggccga tgacgcgggg cgcctatgag ggcggcggtt tcatcaagac
     2341 cgatccggcg ctggcgcatc ccgatatcca gatcggcgtg ggcctg
//
LOCUS       DAOMEO010000468         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4749_length_2385_cov_2.901288, whole genome shotgun sequence.
ACCESSION   DAOMEO010000468 DAOMEO010000000
VERSION     DAOMEO010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_2385_cov_2.901288"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLH31_10770"
     CDS             <1..499
                     /locus_tag="PLH31_10770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQR89718.1"
                     /translation="LLAAVAKAAEVEIAFSNGWRYGAPVAPGPVTLGDLYNIVPMNPL
                     ISRTTLTGAELKAMLEENLERTFAADPYEQMGGYIKRCRGLTAFVKLENPKGQRLDRL
                     LIGGRPVEPDGDYRVAFITAQGVPKRYGRDRQTLDISAVEALETWFADHTPGETDLPA
                     SVLII"
     gene            511..1128
                     /locus_tag="PLH31_10775"
     CDS             511..1128
                     /locus_tag="PLH31_10775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR89719.1"
                     /translation="MDPITARDFWNNRYDRQDYLFGETPNAFLAAQINRLTPGMTALA
                     VADGEGRNGVWLAEQGLAVTTTDIAPRAIEKALALAERRGVTLDAQLADLEIWAWPQD
                     AFDVVVAVFIQFAPPPQRDRLFERMRAAVKPGGLILLQGYRPEQIAYGTGGPGQVENL
                     YTEALLRDAFADFDILHLNSHDSDVSEGAGHSGRSALIDLVARRP"
     gene            1158..1682
                     /locus_tag="PLH31_10780"
     CDS             1158..1682
                     /locus_tag="PLH31_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQR89720.1"
                     /translation="MKTLLLALSLAASLAASPSFAQAVSGFGGFKPLPEAGEQPNPSR
                     VYRVIFDVSQGGPDDKPLKGLDRVARLANMLAAGGVDADHRQIVVVLHGAATPAVLSD
                     AAWAARGKGDANPNSALIRSLIAAGVQVRLCGQAMAGNGIMQADLEPGVSVDLAALMT
                     VIHHQQAGYALVVN"
     gene            1783..>2385
                     /locus_tag="PLH31_10785"
     CDS             1783..>2385
                     /locus_tag="PLH31_10785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR89721.1"
                     /translation="MTGLPRLNEPAPDFHARTTHGDRSLADYRGKWLILFSHPSDFTP
                     VCTTEFIGFAKHADEFAAMNCELLGLSIDSIFSHLAWTRNINEKFGVEIPFPIIEDLK
                     MEVAQAYGMIHPGAADTSAVRATFLIDPEGKLRAMVYYPMSNGRSIEEFVRLLTALQA
                     SDANGVATPENWRPGQPAIVPPPKTAEAAEARASEGYVYTD"
BASE COUNT          418 a          849 c          720 g          398 t
ORIGIN      
        1 tcttctggcg gcggtcgcca aggcggccga ggtcgagatc gctttctcca acggctggcg
       61 ctacggcgcg ccggtcgcgc cgggtccggt caccttgggc gacctctaca acatcgtgcc
      121 gatgaatccc ctgatctcgc gcacgacgct gaccggagcc gagctgaagg ccatgctcga
      181 agagaatttg gaacgcacct tcgccgcgga cccgtacgaa cagatgggcg gctatatcaa
      241 acgctgccgg gggctgacgg ctttcgtcaa gcttgagaat ccgaaggggc agcgtctcga
      301 ccgcctcctg atcggaggtc gcccggtcga gccggacggg gattacaggg tcgctttcat
      361 caccgcccag ggcgtgccta aacgctacgg ccgcgatcgc cagacgctgg acatatccgc
      421 tgtcgaagcg ctggagactt ggttcgccga tcatacgccc ggcgagaccg atttgcccgc
      481 cagcgtcctg atcatttgaa agaatcccct atggacccca tcaccgcccg cgacttctgg
      541 aacaaccgct acgaccgtca ggactatctg ttcggcgaaa cccccaatgc cttcctggcc
      601 gcccaaatca accgactgac gccgggcatg acggcgctgg cggtcgccga cggcgagggg
      661 cgaaacggcg tgtggctcgc cgaacagggt ctggccgtca ccaccacgga tatcgcccca
      721 cgcgcgatcg agaaggccct cgccctggcc gagcgacgcg gggtcacact ggacgcccag
      781 ctggcggacc tcgagatctg ggcctggccg caggatgcgt tcgacgtcgt ggtcgccgtc
      841 ttcattcagt tcgccccgcc tccccagcgc gaccgacttt tcgagcgtat gagggcggcc
      901 gtgaagcctg gcggcctgat cctgctgcag ggctatcggc cggaacagat cgcctatgga
      961 accggcggcc cggggcaggt cgagaacctc tacaccgagg cgctgctccg cgacgccttc
     1021 gccgacttcg acatcctgca tctgaatagc cacgacagcg acgtctccga aggcgccggc
     1081 cacagcggcc ggtccgccct gatcgatctc gtggcccgcc gtccctgacc cccagcgcct
     1141 gcacaccgga actcgccatg aaaaccctcc tcctcgccct gtccctggcc gcctccctgg
     1201 cggcctcccc aagcttcgcc caggctgtct ctggcttcgg cggcttcaag cccttgcccg
     1261 aggccggcga acagccgaat ccttcgcgcg tctatcgcgt catcttcgac gtttcccagg
     1321 gcggtccgga cgacaagccg ctgaagggac tggatcgcgt cgcacggctg gcaaatatgc
     1381 tggcggcggg cggcgtggac gccgaccatc gtcagatcgt ggtcgtcctg cacggcgccg
     1441 ccacccctgc ggtcctgtcc gacgccgcct gggccgcacg cggcaagggg gacgccaacc
     1501 ccaacagcgc cctgatccgc agcctcatcg ccgccggcgt ccaggttcgg ctgtgcggcc
     1561 aggccatggc ggggaacggc ataatgcaag ccgacctcga accgggcgtg agcgtcgatc
     1621 tcgccgccct gatgacggtc atccaccacc agcaggctgg atatgccctg gttgtgaact
     1681 gacgggctcg gcatagacgg aaccttccct ggccataaga actattagtt tgtgtaagtg
     1741 tgtatgtaag tgttcaatgg gacgtctgaa ggagacatcg acatgaccgg actgccccgc
     1801 ctcaacgaac ccgcccccga ctttcacgcc cgcaccacgc acggggatcg cagcctggcc
     1861 gattaccgcg gcaaatggct gatcctcttc tcccacccgt cggacttcac cccggtctgc
     1921 acgaccgagt tcatcggctt cgccaagcac gcggatgagt tcgccgcgat gaactgcgag
     1981 ctcctggggc tctcgatcga cagcatcttc tcgcacctgg cctggacccg gaacatcaat
     2041 gagaagttcg gcgtcgaaat ccccttcccc atcatcgagg atctgaagat ggaggtggcc
     2101 caggcctacg ggatgatcca ccccggggcg gccgacactt cggctgttcg cgccaccttc
     2161 ctgatcgatc cggaaggcaa gctgcgcgcc atggtctact atcccatgag caatggccgc
     2221 agcatcgaag agttcgttcg gctgctcacc gcgctccagg cgtccgacgc caacggggtc
     2281 gcgacgcctg agaactggcg ccctggccaa ccggccatcg tccccccacc caagaccgcc
     2341 gaggcggctg aggcccgcgc ctccgagggt tacgtctaca ccgac
//
LOCUS       DAOMEO010000469         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4750_length_2385_cov_2.279399, whole genome shotgun sequence.
ACCESSION   DAOMEO010000469 DAOMEO010000000
VERSION     DAOMEO010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4750_length_2385_cov_2.279399"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /locus_tag="PLH31_10790"
     CDS             complement(<1..2336)
                     /locus_tag="PLH31_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89722.1"
                     /translation="MFPYDVAARHRTDGLKGAFGREEALRKLIEGADLEIASANAQVI
                     TLRARTAGPLGAAVADVVEEVIVTSRAGSDARTRLATSYAVTSLNEETLRMRSPMGVA
                     DALKSVPGFWVEASGGEASANIRARGIPQEGFSAIALQEDGAPVQHDGGLGYLNADQS
                     FRLDETIERIEVVRGGPSSIFASYAPGGTVNFITRKGGETPEGIAKVQVSDYGLKRVD
                     LWYGGPIGEWRVGAGGFWREDDGIRDPGFTANKGYQWRVSAGRDFERGSIEFNIKHLD
                     DNVILFAGVPLKFNASGEPGDTPGFDALKGTLAGPETAQLTLRGPTGPFNWDLTRGTE
                     VKLTQATAILNYELFDGWNFQNTLRYRTSESKRIGLFPNTPLTGAARIAQYRALLLAA
                     TPGATDVQLRYASSPTEIFQTTGAGQNGNGLVLDGSLRYVSVPLDELINDARLLHKFE
                     VGGQTHDVAFGVYTAHVEEAFNRYSANVLLDVRDNARRLNLVAVNGAGQVLSSLTENG
                     ISRYGAEFANGDGKSNTTALYLTDEWSITPSLRIDAGVRWEQIEFEGRSERTKTVNLG
                     LSPTPADDTVLAGSGVYDRFDRKFDHAGWTVGADYRITPQMGVFARYTSAFRLPSLGD
                     YITNALNTAPTVQTMDLMEAGYKWSTPKADLYATVFQTTYDNLGFGSLVFNPATGAYV
                     QQTSITETETLGLELEGTVRPTRWFDVSFSATVQNPEFGDYKFEEACSISATDATCQI
                     KPPAATASRTRDFTGNQLIRVPKTSFRLTPGVNLLDGRL"
BASE COUNT          374 a          748 c          857 g          406 t
ORIGIN      
        1 aggcgcccat ccagcaggtt cacgccgggc gtcagccgga acgaggtctt cggtacacgg
       61 atcaactggt ttccagtgaa gtcgcgcgtg cggctggcgg tcgcggcggg cggcttgatc
      121 tggcaggtgg cgtccgtcgc cgagatcgag caggcctcct cgaacttgta gtcgccaaat
      181 tccggattct ggacggtcgc gctgaagctc acgtcgaacc agcgggtggg acgcacggtc
      241 ccctccagtt ccaggcccag agtctcggtc tcggtgatgc tggtctgctg gacataggcg
      301 ccggtggccg gattgaacac caggctgccg aagccgaggt tgtcataggt ggtctggaac
      361 accgtggcgt agaggtcggc cttgggggtc gaccacttat agccagcttc catcaggtcc
      421 atggtctgga cggtcggggc cgtattgagg gcgttggtga tgtaatcgcc cagcgacggc
      481 agacggaagg ccgaggtgta gcgcgcgaag acgcccatct gcggcgtgat ccggtagtcg
      541 gcgcccacgg tccagccggc atggtcgaac ttgcggtcaa aacgatcata gacgcccgaa
      601 ccggccagga cggtgtcgtc ggccggggtc ggcgagagac ccagattgac ggtctttgtg
      661 cgctcgctgc gaccttcgaa ttcgatctgc tcccagcgca cgccggcgtc gatgcgcagg
      721 gacggcgtga tgctccactc gtcggtcagg tagagcgcgg tggtgttgga cttgccgtcg
      781 ccgttggcga actcggcgcc atagcgcgag atgccgttct cggtgaggct ggacagcacc
      841 tgacccgcgc cgttgacggc caccaggttc agccgccggg cgttgtcgcg cacgtcgagc
      901 agaacgttgg ccgaatagcg gttgaaggct tcctcgacgt gggcggtgta gaccccgaag
      961 gcgacgtcgt gggtctggcc gccgacttcg aacttgtgca gcaggcgggc gtcattgatc
     1021 agttcgtcca gcgggaccga gacgtagcgc agcgagccgt cgaggacgag gccgttgccg
     1081 ttctgaccag cgcccgtggt ctggaagatc tcggtcggcg aactggcgta gcgcagctgc
     1141 acgtcggtgg cgcccggcgt ggccgccagc aggagggcgc ggtactgggc gatgcgagcc
     1201 gcaccggtca agggcgtgtt ggggaacagg ccgatacgct tggactccga cgtgcggtag
     1261 cgcagcgtgt tctggaagtt ccagccgtcg aagagctcat agttcaggat ggcggtggcc
     1321 tgggtcagct tgacctcggt gccgcgcgtc aggtcccagt tgaagggccc cgtcggaccg
     1381 cgcagggtca gctgggcggt ttccggaccg gccagggtgc ctttcagcgc gtcgaagcca
     1441 ggggtgtcgc ccggctcgcc cgaagcgttg aacttcaggg gcacgccggc gaacaggatg
     1501 acattgtcgt ccaggtgctt gatgttgaac tcgatgctgc cgcgctcgaa atcgcgaccg
     1561 gccgagactc gccactgata gcccttgttg gccgtgaagc ccgggtcgcg gatgccgtca
     1621 tcctcgcgcc agaagccgcc ggcgccgacg cgccactcgc cgatcgggcc gccgtaccag
     1681 agatcgacgc gcttgaggcc gtagtcgctg acctgaacct tggcgatgcc ttccggggtc
     1741 tcgccgccct tgcgggtgat gaagttgacc gtgccaccgg gggcgtagga ggcgaagatc
     1801 gacgagggac caccgcgcac gacttcgatg cgctcgatgg tctcgtccag gcggaacgac
     1861 tggtcggcgt tcaaatagcc caggccgccg tcgtgctgca ccggcgcgcc gtcttcctgc
     1921 agggcgatgg ccgagaaccc ttcctgcggg atgccccgcg cgcggatgtt ggcgctggcc
     1981 tcgccgcccg aggcctcgac ccagaagcct ggcaccgact tcagggcgtc cgcgacgccc
     2041 atcggggagc gcatccgcag ggtttcctcg ttcaggctgg tgacggcgta gctggtggcc
     2101 aggcgcgtgc gggcgtcaga accggcgcgc gaggtgacga tcacctcttc caccacgtca
     2161 gcgacggccg cgcccaaagg gcccgccgtc cgggcgcgca gcgtgatgac ctgcgcattg
     2221 gccgaggcga tctccaggtc ggcgccctcg atcagcttgc gcagggcttc ctcacgacca
     2281 aacgcaccct tcagaccgtc ggtgcggtga cgggcggcga cgtcgtaggg gaacatcagc
     2341 tgtacgccgg cctgacgcga gaaggcgttc agcgccgccg ccgtg
//
LOCUS       DAOMEO010000470         2380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4762_length_2380_cov_1.959140, whole genome shotgun sequence.
ACCESSION   DAOMEO010000470 DAOMEO010000000
VERSION     DAOMEO010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_2380_cov_1.959140"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLH31_10795"
     CDS             complement(<1..798)
                     /locus_tag="PLH31_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQR89723.1"
                     /translation="MRVGVVGCGVGGMAAALALARRGHEVTLLEAFAQPRPLGSGLLL
                     QPTGLAALKALGLDDAIRAAGARVDRLQGRDTRGRTVMNLDYADWRPGAHGVGIHRAV
                     LFDALHGRLAGAGVTVVTSARVIRVEDPARPVLQDEDGRAFGPFDLAIIGDGSASTLR
                     RAIRPGARAPLYPWGALWVNAPDPHGQFSGALRQVYHRAEMMAGVLPVGHGPDGGQGQ
                     VSIFWSLPTADFDRALAIDFNDWCASRLGAIWPEAAALLAGQADWSGF"
     gene            complement(804..>1200)
                     /locus_tag="PLH31_10800"
     CDS             complement(804..>1200)
                     /locus_tag="PLH31_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQR89724.1"
                     /translation="GTGGTISGVGQVLKARKPGLKMVAVEPEASAVLSGGAPGPHKIQ
                     GIGAGFVPGILDRGVIDEIVQVSNDDAFAMARRSAAIEGLPVGISSGAALTAAFDLAL
                     RDDYAGKLIVAIIPSFAERYLSTALFDGL"
     assembly_gap    1201..1210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1211..1739)
                     /locus_tag="PLH31_10805"
     CDS             complement(<1211..1739)
                     /locus_tag="PLH31_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQR89725.1"
                     /translation="MADATSSYDAARYKRSGRGKIYDSILDTIGDTPLIRLPRLSAEI
                     QPKGEVVAKLEFFNPLASVKDRIGVSLIESLEAQGILTAGATIVEPTSGNTGIALAFV
                     AAAKGYKLILVMPESMSIERRKMLLLLGAKLELTPAEKGMRGAVARAQEIIEATPGAV
                     MPQQFENAANPLIHRV"
     gene            complement(1810..>2102)
                     /locus_tag="PLH31_10810"
     CDS             complement(1810..>2102)
                     /locus_tag="PLH31_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HQR89726.1"
                     /translation="AEARFDGLAGCLRTPRGGSSRQTLLVIDEGQVRSRLISPREGAR
                     LMGLPDTYALPGSQTAGLHVIGDGVAAPVVRWLAEGLLEPLIDSKSASLAAE"
     assembly_gap    2103..2112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2113..2353)
                     /locus_tag="PLH31_10815"
                     /pseudo
     CDS             complement(2113..2353)
                     /locus_tag="PLH31_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617254.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          358 a          790 c          859 g          353 t
ORIGIN      
        1 aaagccgctc cagtcggcct ggccggccag cagggcggcg gcctctggcc agatcgcgcc
       61 gagcctgctg gcgcaccagt cgttgaaatc gatcgccagg gccctgtcga agtcggctgt
      121 gggcagcgac cagaatatcg agacctggcc ctggccaccg tcgggcccat gcccgaccgg
      181 cagcacgccg gccatcatct cggcgcggtg atagacctgt ctcaaggcgc cggagaactg
      241 gccgtgggga tcaggagcgt tcacccacag cgcgccccat ggatagagcg gcgcgcgggc
      301 tccaggccgg atcgccctgc gcagggtcga ggccgagccg tcaccgatga tcgccaggtc
      361 gaaggggccg aacgcgcggc catcctcgtc ctgcagaacg gggcgggcgg gatcctcgac
      421 ccggatgact cgcgcgctcg tgaccaccgt gacgccggcg ccggcaagtc tgccatgcag
      481 cgcatcgaac agcacggccc ggtggatgcc gacgccatgg gcgccgggcc gccagtcggc
      541 atagtcgagg ttcatcaccg tgcggccccg cgtatcgcga ccctgcaggc ggtcgacccg
      601 ggcgccggcg gcgcggatgg cgtcgtccag ccccagcgcc ttcagggccg ccagaccggt
      661 gggttggagg agcaggcccg agcctagcgg gcgtggctgc gcgaaggcct ccagcagggt
      721 gacctcatgg ccccggcgcg ccagggcgag ggcggcggcc atgccgccca ccccgcatcc
      781 gacgacgccg acgcgcaagg gggctacaac ccgtcgaaca gggccgtgga caggtagcgc
      841 tcggcgaagc tggggatgat cgccacgatc agcttgccgg catagtcgtc gcgcagggcc
      901 aggtcgaagg cggcggtgag ggccgcgccg gagctgatgc cgaccggcag gccttctatg
      961 gcggcggagc gacgggccat ggcgaaggcg tcgtcattgc tgacctgcac gatctcgtcg
     1021 atgacgccgc gatccaggat gcccggcacg aagccggcgc cgatgccctg gatcttgtga
     1081 gggccgggtg cgccgcccga caggacggcc gaggcttccg gctcgacggc caccatcttg
     1141 aggccgggct tgcgcgcctt cagcacctgg ccgacgccgg aaatggtgcc gcccgtgccg
     1201 nnnnnnnnnn agacgcggtg gatcagcgga ttggcggcgt tctcaaactg ctggggcatc
     1261 accgcgccgg gggtggcctc gatgatttcc tgggcgcggg cgacggcgcc gcgcatgccc
     1321 ttctcggccg gggtcagctc cagcttggcg cccagcagca gcaacatctt gcggcgctcg
     1381 atcgacatgc tttccggcat gaccaggatc agcttgtagc ccttggcggc ggccacgaag
     1441 gccagggcga tgccggtatt gccgctggta ggctcaacga tagtggcgcc agccgtcagg
     1501 atgccctggg cttccaggct ctcgatcagc gagacgccga tgcggtcctt caccgaagcc
     1561 agcggattga agaactccag cttggccacg acctcgccct tgggctggat ttccgccgac
     1621 aggcggggaa gccggatcag cggggtgtcg ccgatggtgt ccaggatcga gtcatagatc
     1681 ttgccgcgtc cggaacgctt gtaacgggcc gcgtcgtagg aggaggtggc gtcggccata
     1741 ggacgatctc gctgaaggtt ttgcaggttt gaacgcacct tacggcgcgg ggttttgcct
     1801 gtcagcgggt cattcggcgg cgaggcttgc agatttgcta tcgatcagag gctccagcaa
     1861 gccttccgcc aaccagcgca ccacgggcgc ggcgacgccg tcgccgatca cgtgcagccc
     1921 ggcggtctgg ctgccgggca gggcataggt gtcgggcagg cccatcagcc gcgccccctc
     1981 ccgggggctg atcaggcgcg agcgcacctg gccctcgtcg atcaccagga gggtctggcg
     2041 cgaggagccg ccgcgcgggg tgcgcagaca gccggccaga ccgtcgaagc gcgcctctgc
     2101 ccnnnnnnnn nncagctcca actgcccgcc gaccgctctt caagtgcgct tcgacggtgg
     2161 cgcggtgagc gggggccatc agggcaagca gggcctgggt cctgtcgggc gtgttccaag
     2221 cgaccgcgtc gtcgggttcg agcatggcgg ccagatcggt gttgcgcggg tgcggtgcgg
     2281 ccaggcccca ccagatccag tcggcctgca gagcggcggg caggcgcgca tgggccgcct
     2341 tgaccgccct ggcttctttt ttgtatttat tgtcttaggt
//
LOCUS       DAOMEO010000471         2375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4771_length_2375_cov_2.643534, whole genome shotgun sequence.
ACCESSION   DAOMEO010000471 DAOMEO010000000
VERSION     DAOMEO010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_2375_cov_2.643534"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /gene="gyrB"
                     /locus_tag="PLH31_10820"
     CDS             <1..2334
                     /gene="gyrB"
                     /locus_tag="PLH31_10820"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQR89727.1"
                     /translation="GMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATKVEVILNADG
                     SVTVTDDGRGIPVDIHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNA
                     LSDWLELKIHRNGHSYQMRFERGDAVTSLAQTGDSPVRAEGPKAGTTLTGTEVTFFPS
                     RDTFAFIEFDRKTLEHRLRELAFLNSGVTIYFRDLRDAEPWEEKLHYEGGIEAFVRHL
                     DKAKTPLLKMPIVIRGRKDKVEVDLALWWNDSYHEHMLCFTNNIPQRDGGTHLSAFRG
                     ALTRIITNYAESSGITKKEKVSVGGEDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEV
                     RPAVEGLVQEGLASWFEEHPNEAKAIVSKIAEAAAAREAARKARELTRRKSALDITSL
                     PGKLADCSERDPAKSEIFIVEGDSAGGSAKQARNRDNQAVLPLRGKILNVERARFDKM
                     LSSDQIGTLITALGAGIGRDDFNADKVRYHKIVLMTDADVDGAHIRTLLLTFFYRQMP
                     ELIERGYIYIAQPPLYKAAKGKSSRYLKDDAEMDAFLIDEGVDGAELDLPSGERRTGK
                     DLLALIQLCRQAKGNIDRLAARAPAFAVEQTALAGLLGETPDIAAAAARLDLYAEEGD
                     GLWTGERGDTAYVFKRIRRGVTESVVLDDGLLHAADARRLAERAGALAEIFSGRAVFR
                     RRDKSTTVRGPMDLVGAVMEAGRKGLSIQRYKGLGEMNPDQLWETTLDAEARTLLQVK
                     VNHADDADDMFSRLMGDLVEPRREFIQENALDAEVDV"
BASE COUNT          440 a          814 c          769 g          352 t
ORIGIN      
        1 ggcatgtata tcggcgacac cgatgacggc tcgggcctgc accacatggt ctatgaggtg
       61 gtcgacaacg ccatcgacga ggccctggcc ggtcacgcca ccaaggtgga ggtgatcctc
      121 aacgccgacg gctcggtgac ggtcaccgac gacgggcgcg gcatccccgt cgacatccac
      181 gaaggcgaag gcgtttcggc ggccgaggtc atcatgaccc agctgcacgc cggcggtaag
      241 ttcgaccaga acagctacaa ggtctcgggc ggtctgcacg gggttggcgt ttccgtcgtc
      301 aacgcgctca gcgactggct ggagctgaag atccaccgta acggccacag ctatcagatg
      361 cgcttcgagc gcggcgacgc ggtgacctcg ctggcccaga ccggcgactc gccggtgcgg
      421 gcggaaggcc ccaaggccgg aaccaccctg acgggcacgg aagtcacctt cttcccgtcc
      481 agggacacct ttgcgttcat cgaatttgac cgcaagacgc tggagcaccg cctgcgcgag
      541 ctggccttcc tgaactcggg cgtgaccatc tatttccgcg acctgcgcga cgccgagccc
      601 tgggaagaaa agctgcacta tgagggcggc atcgaggcct ttgtccgaca cctcgacaag
      661 gccaagacgc ccctgctgaa gatgccgatc gtcattcgcg gccgtaagga caaggtcgag
      721 gtcgatctgg ccctgtggtg gaacgacagc tatcacgagc acatgctgtg cttcaccaac
      781 aacatcccgc agcgggatgg cggcacgcac ctttcggcct tccgcggcgc cctgacccgg
      841 atcatcacca actacgccga gagctcgggc atcacgaaga aggaaaaggt cagcgtcggg
      901 ggcgaggacg cccgcgaagg cctgacttgc gtactgtcgg tcaaggtgcc ggaccccaag
      961 ttcagctcgc agaccaagga caagctggtc tcgtccgaag tgcgccccgc cgttgaaggt
     1021 ctggttcagg agggcctggc cagctggttc gaggagcatc cgaacgaggc gaaagccatc
     1081 gtctccaaga tcgccgaggc cgccgccgcc cgcgaagccg cccgcaaggc ccgcgaactg
     1141 acccgccgca agagcgcgct cgacatcacc tccttgcccg gcaagctggc cgactgttcg
     1201 gaacgcgatc cggccaagtc cgagatcttc atcgtcgagg gtgactcggc cggcggttcc
     1261 gccaagcagg cccgcaaccg cgacaaccag gcggtgctgc ccctgcgcgg caagatcctc
     1321 aacgtcgaac gggcccgctt cgacaagatg ctgtcgtccg accagatcgg caccctgatc
     1381 accgctctgg gcgccggcat cggccgcgac gacttcaacg ccgacaaggt gcgctaccac
     1441 aagatcgtgc tgatgaccga cgccgacgtc gacggcgccc acatccgcac cctgctgctg
     1501 accttcttct accggcagat gcccgagctg atcgagcgcg gctacatcta tatcgcccag
     1561 ccgccgctct ataaggcggc caagggcaag tcctcgcgct acctcaagga cgacgccgag
     1621 atggacgcct tcctgatcga cgagggtgtc gacggggcgg agcttgacct gccctcgggc
     1681 gagcgccgca ccggcaagga cctgctggcc ctgatccagc tgtgccgtca ggccaagggc
     1741 aatatcgatc gcctggccgc ccgcgccccc gccttcgccg tggagcagac ggccctggcc
     1801 ggcctgctgg gcgagacccc cgacatcgcc gccgccgccg cgcgtctgga cctctatgcc
     1861 gaggaaggcg atggcctgtg gaccggcgag cgtggcgaca cggcctatgt gttcaagcgc
     1921 atccgtcgtg gcgtgaccga gagcgtggtg ctggacgatg gcctgctgca cgccgccgac
     1981 gcccgtcgcc tggccgaacg ggccggggcc ctggccgaga tcttcagcgg ccgcgccgtg
     2041 ttccgtcgcc gggacaagtc gacgaccgtc cgtggaccga tggatctggt cggcgcggtg
     2101 atggaggccg gccgcaaggg cctgtcgatc cagcgctaca agggcctggg cgagatgaac
     2161 cccgaccagc tgtgggaaac caccctcgac gccgaggccc gcaccctgct gcaggtcaag
     2221 gtcaaccacg ccgacgacgc cgacgacatg ttcagccgtc tgatgggcga tctggtcgag
     2281 ccgcgccgcg agttcatcca ggaaaacgcg ctggacgccg aggtcgacgt ctgacgacgt
     2341 cgggatcgct ggaccggctc actatgaatg agaag
//
LOCUS       DAOMEO010000472         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4782_length_2371_cov_2.479275, whole genome shotgun sequence.
ACCESSION   DAOMEO010000472 DAOMEO010000000
VERSION     DAOMEO010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_2371_cov_2.479275"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /locus_tag="PLH31_10825"
     CDS             <1..85
                     /locus_tag="PLH31_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89728.1"
                     /translation="NELFFRDNTMMLFGDAKKMVEGIVKGL"
     gene            complement(172..519)
                     /locus_tag="PLH31_10830"
     CDS             complement(172..519)
                     /locus_tag="PLH31_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HQR89729.1"
                     /translation="MRKFIMSLTTVASLSLAAIPVLGLAQAAQAGEHKARIAVSDLNL
                     SNPAQAAVFNARVDDAARTLCRAKVRNNDLKMSLNDCHMSVKREASRQLSATQRKALK
                     FASRAASVEMAAR"
     assembly_gap    669..678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1179..1922
                     /locus_tag="PLH31_10835"
     CDS             1179..1922
                     /locus_tag="PLH31_10835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR89730.1"
                     /translation="MTSQTLAARPPYSRIAFLALVGVIATVGALNAGSALRAFAGEPA
                     PAPAPVVEAPPVEAPPPPPPAFVFDTPLPGRVVNSPFGLRQLPWEENGRLHEGVDIAA
                     PAGSAIRAATDGVVVRTGASGSYGRFVEIAHKDGFRTMYAHLGATARGVKPGLYAKRG
                     QTIAYVGSSGRSTGAHLHFELRRKGKPLNPAFFLGQTFAQADDLPLRAAARGPRKVRM
                     AQVSNWPDGVKPAKTKGGRVQTRLSVADS"
     gene            1968..>2371
                     /locus_tag="PLH31_10840"
     CDS             1968..>2371
                     /locus_tag="PLH31_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR89731.1"
                     /translation="MLKVLVRGFAGVAAFLVSYIALGGVMSAASAPRPSGEMVQIDPG
                     RSLRLVCEGPSSDRPVVWLEAGAFGFAADWGATQEALTAAGWRSCAYDRAGMGYSPSG
                     PAPRDGLAIVSDFEKLTAASGEPGPYILVGHS"
BASE COUNT          377 a          737 c          783 g          464 t
ORIGIN      
        1 gaacgagctg ttcttccgcg acaacacgat gatgctgttt ggcgacgcca agaagatggt
       61 cgaggggatc gtgaaggggc tttgatccgc cacgttccgc agtccagatg aagcaaaggc
      121 ctcaggtgat cctgaggcct ttgtcgtttt cggatgtcgg cgagcctgag atcaccgggc
      181 ggccatctcg accgacgcag cgcgggaggc gaacttcagg gccttgcgct gcgtggccga
      241 cagctggcgg ctggcttcac gctttaccga catatggcag tcgttgagcg acattttcag
      301 gtcgttgttg cgaaccttgg cgcggcatag ggtccgggcg gcgtcatcga cccgggcgtt
      361 gaacacggcg gcctgggccg ggttcgacag gttcaggtcg ctgaccgcga tgcgggcttt
      421 gtgctcgccg gcttgtgcgg cctgggccag gcccagaacc ggaatggcgg ccaggctcag
      481 gctggcgaca gtggtaaggc tcatgatgaa cttgcgcatt tgaagtaatc cctcggcttt
      541 ggtttgtgtc tgacgctttg ttgcgccccg tgtccaaaga tgtactttcg agcacccata
      601 aagtcccgtt acgtctcggt catgacgttt aatttaaaga attacacgat tttaacagaa
      661 taaaattgnn nnnnnnnnag taatgtccgg ccgtctattg tcctgcggca ggggcgggta
      721 ttgacgcgcg gtccggcgac acgcttgtcg cgacagggtg tcgcacgcgt gccggggata
      781 gatggctcgg cgcagggcct gctgtcctgg tccaggtcgc gcttaatctg ccttgaggct
      841 ccgagatatt tgcgcgcccg aagcctgcgc aaacattgcc aagtgtgcag aggtgagacc
      901 ggttgcgaat ttccgatacg tgccggctaa gctattgaag ggcggcaagc gaatattttc
      961 gcatccgcaa atcatggggt cgagtgctgg aaatcctgat gcctatagac atgatcagcg
     1021 ggcccggaaa acggcagact gtcaagctgc gcccagcttt cgcccaatag aggggtcatc
     1081 aagcccgtcg attcattatc acgaaaggtc gatgtgggac caagtgcgcc ttcgcacggt
     1141 cccgcagccc gactgctcgt ctctgaggaa gcggattttt gacgtcacag acccttgccg
     1201 cccgaccgcc ctattcccgt atcgcctttc ttgccctggt gggcgtgatc gccacggtgg
     1261 gcgcgctgaa cgcgggctcg gccctgcgcg ccttcgccgg tgaacccgct ccggcgccag
     1321 cgccggtcgt tgaggccccg ccggtcgaag ccccgccgcc gccgccgccg gccttcgtgt
     1381 tcgacacccc attgccgggc cgcgtggtca attcgccgtt cggtctgcgc caactgccct
     1441 gggaagagaa cggccgcctg catgagggcg tcgacatcgc cgcgccggcc ggttcggcga
     1501 tcagggccgc caccgacggc gtggtcgtcc ggacgggcgc cagcggctcc tatggccgtt
     1561 tcgtggagat cgcccacaag gatggcttcc gtacgatgta cgcccatctg ggcgccacgg
     1621 cgcggggtgt gaagcctggc ctgtacgcca agcgcggcca gacgatcgcc tatgttggca
     1681 gcagtggccg ctcgaccggc gcgcacctgc attttgaatt gcgccgcaag ggcaagccgc
     1741 tgaaccccgc gttcttcctg ggccagacct tcgcgcaggc cgacgatctg ccgctgcgcg
     1801 ccgcagcccg cgggccgcgc aaggtgcgga tggctcaggt gtccaattgg ccagacggcg
     1861 tcaagccggc caagaccaag ggcggccggg tccagactcg tctgtcggtc gccgatagct
     1921 gagacaaggc ggctcgacgc gtcgcaatcg cccggtaatg tagggccatg ctcaaggtcc
     1981 ttgttcgcgg tttcgccggt gtcgctgcct ttctcgtttc ctatatcgcc ctgggtggcg
     2041 tgatgagcgc cgccagcgcg cctcgtccta gcggcgagat ggtccagatc gatccgggac
     2101 gcagcctgcg tctggtttgc gaaggcccca gcagcgatcg cccggtggtc tggctggagg
     2161 ctggcgcctt tggcttcgcc gccgactggg gcgcgaccca ggaggccctg acggcggccg
     2221 gctggcgctc ctgcgcctat gaccgggccg gcatgggcta ttcgccgtcg ggacccgcgc
     2281 cgcgtgacgg ccttgccatc gtctccgact tcgagaagct gaccgccgcc tcgggcgagc
     2341 cggggcccta tattctggtc ggccactcga t
//
LOCUS       DAOMEO010000473         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4783_length_2371_cov_2.233161, whole genome shotgun sequence.
ACCESSION   DAOMEO010000473 DAOMEO010000000
VERSION     DAOMEO010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_2371_cov_2.233161"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="gspK"
                     /locus_tag="PLH31_10845"
     CDS             <1..880
                     /gene="gspK"
                     /locus_tag="PLH31_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HQR89732.1"
                     /translation="RFGIRRTFNARVVNQAQWYALGTEQLARARIGSLMDRDASKTTL
                     EGGWNGRVIAFPIEQGRMEATLTDATGCFNLNSVVEANQGEPFRRRDLGVRQFSTLLS
                     LVGAPNERELAENLAEWMDSNDVGRAEAEDQGYVNLPQPYRTAGGPLAEVSELRAVRG
                     FTPAIYAALRPYVCALPTTDLSPINVNTLPSERALLLTALTEGKVARETARGVLDRRP
                     TQGWPDAQAFWGEQALLTTPPGDVVREQIRFRTRFFALNSQVVFADMPIFMSSLFETR
                     SASTVTLVARRWTSDE"
     gene            877..1962
                     /gene="gspL"
                     /locus_tag="PLH31_10850"
     CDS             877..1962
                     /gene="gspL"
                     /locus_tag="PLH31_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HQR89733.1"
                     /translation="MTVLRLLILPPQSGARLELIEAEGDVVSRRPVEIDERLDVGAVL
                     VAPGADTVARWMDLPVGSPAQARSAAAFRVEDEIALAGEELHIAVGGLDEAGRTLVVW
                     TARAKLNAWLDMALAHGVTPEAVIPDYLLLPEDPAGQLIVAPFGERLGLREPGRAFNV
                     EPELAALLAADRPHRYIPAAELDPWLAAGARSPAINLLQGQFAIRSGAPARGRKRLAI
                     LAAVAVISPLILVLAQIAHDQLTARALERQARSLAVSMVPQASRYEDPAAFALARITA
                     AQGRQGFGDLAASYLAMVRSAPGVTLDTLVYGDDGAIRSAIAYANYSDMDQLRNAAKK
                     AGLQLTEQSTVTEGARITSDLIVRRQP"
     gene            1959..>2371
                     /gene="gspM"
                     /locus_tag="PLH31_10855"
     CDS             1959..>2371
                     /gene="gspM"
                     /locus_tag="PLH31_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HQR89734.1"
                     /translation="MIERIDAARQWWLSRSDRERALLAVMLVAVLGVVGWYGVISPLR
                     SAAEDSQQRVETAARKLASLKAASRENAPQPGQSAAAPQVLVEASAIKAGVPIARRRQ
                     DANGQFTIWITAIDARTLLPWVAAVEREGGVTVAD"
BASE COUNT          357 a          803 c          838 g          373 t
ORIGIN      
        1 ccgctttggc atccgccgca cctttaacgc gcgcgtcgtc aaccaggccc agtggtacgc
       61 cctgggaacc gaacaactgg cccgcgcccg catcggcagc ctgatggatc gcgacgccag
      121 caagaccacg cttgagggcg gctggaacgg tcgggtgatc gcctttccga tcgagcaggg
      181 ccgcatggag gcgaccctga ccgacgccac gggatgcttc aacctcaaca gcgtggtgga
      241 ggccaatcag ggcgagccct tccgccgccg tgacctgggc gttcgccagt tttcgaccct
      301 gctgtcgctg gtcggcgcgc cgaacgagcg cgagctggcc gagaacctgg ccgaatggat
      361 ggacagcaac gacgtcggcc gcgccgaagc cgaggatcag ggctacgtca atctgcccca
      421 gccctacaga accgccggcg ggccgctggc cgaggtcagc gaactgcggg cggtgcgcgg
      481 cttcacgcca gcgatctacg ccgcgctccg gccctatgtc tgtgccctgc cgacgaccga
      541 tctgtcgccg atcaatgtca acaccctgcc gtccgagcgc gcccttctgc tgaccgccct
      601 gaccgagggc aaggtcgccc gggaaacggc gcgcggcgtg ctggatcgtc ggccgaccca
      661 gggctggccc gacgcccagg cgttctgggg tgagcaggcc ttgctgacca cgccgcccgg
      721 cgatgtggtg cgcgagcaga tccgctttcg cacgcgcttt ttcgcgctta acagtcaggt
      781 tgtcttcgcc gacatgccga tcttcatgag ctcacttttt gaaacccgtt cggccagcac
      841 ggttacgctg gtcgcgcgac gctggacctc ggacgaatga ccgtgctgcg cttgctgata
      901 ttgccgccac aaagcggcgc ccggcttgag ttgatcgagg ccgaagggga tgtcgtgtcc
      961 cgtcgccctg tcgagatcga cgagcggctg gatgtcggag ccgtactggt cgcgcccggc
     1021 gccgacaccg tcgcccgctg gatggacctg ccggtcggca gtccggccca ggctcgatcc
     1081 gcagccgcgt ttcgcgtcga ggacgagatc gcgctggctg gcgaggagct gcacatcgct
     1141 gtcggcggtc tggacgaggc gggacgcacg ctggtggtct ggacggcccg cgccaagctt
     1201 aacgcctggc tggatatggc cttggcccac ggcgtcacgc cggaagcggt gatccccgac
     1261 tatctcctgc taccggaaga tccggccggg cagttgatcg tcgcgccatt cggggagcgt
     1321 ctgggcctgc gggagccggg cagggcgttc aatgtcgagc ccgagctggc cgcgttgctc
     1381 gccgcagatc ggccgcatcg ctacattccc gcggcggagc ttgatccgtg gctggcggcg
     1441 ggggcgcggt cgccggcgat caacctgctg cagggccagt tcgcgatccg cagcggcgcg
     1501 ccggcccggg ggcgaaagcg actggccatc ctggcggccg tcgccgtgat ctcgccgctc
     1561 atcctggttc tggcccagat cgcccatgac cagctgaccg cgcgcgcgct cgagcggcag
     1621 gcccgaagcc tggcggtgtc gatggtcccg caggcgtcgc gctatgaaga tccggccgcc
     1681 ttcgccctgg ccaggatcac ggccgcccag ggacgtcagg gctttggcga tctggcggcc
     1741 agttatctgg ccatggtgcg ctcggcgccc ggcgtgacgc tggacaccct ggtctatggc
     1801 gacgacggcg cgatccgctc ggcgatcgcc tatgccaact attcggacat ggaccagctt
     1861 cgcaatgcag cgaagaaggc agggctccag ctgaccgagc agagcacggt caccgaaggc
     1921 gcccgcatca ccagcgatct catcgtcagg agacagccat gatcgagcgg atcgacgccg
     1981 caaggcaatg gtggctgtcg cgttctgacc gggagcgggc gctgctggcc gtcatgctgg
     2041 tcgccgtgct tggcgtcgtg gggtggtatg gcgtcatctc gcccctgcgc agcgccgctg
     2101 aggatagcca gcagcgggtc gagacggcgg cccgaaagct ggcgagcctg aaggcggcct
     2161 cgcgggagaa cgcgcctcag ccgggccaga gcgccgcagc gccccaggtt ctggtcgagg
     2221 ccagcgcgat caaggccggc gtgccgatcg cgcggcggcg gcaggacgcc aacggccagt
     2281 tcacgatctg gatcacggcg atcgacgcgc ggaccctgct gccctgggtg gcggcggttg
     2341 agcgggaggg cggcgtcacg gtggccgact t
//
LOCUS       DAOMEO010000474         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4785_length_2371_cov_1.921848, whole genome shotgun sequence.
ACCESSION   DAOMEO010000474 DAOMEO010000000
VERSION     DAOMEO010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_2371_cov_1.921848"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>319
                     /locus_tag="PLH31_10860"
     CDS             <1..>319
                     /locus_tag="PLH31_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQR89735.1"
                     /translation="AGCSRIFKEKVSGARADRRELNRLLAALRDDDQVVVTRIDRLAR
                     STFDLFAIVRRIVDAKAQFRSLAEPWADTATSTGRLMLAVLGGLADVERDLIRTRTAE
                     GRAR"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            471..701
                     /locus_tag="PLH31_10865"
     CDS             471..701
                     /locus_tag="PLH31_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HQR89736.1"
                     /translation="MSTVQLRRVDPSRNMARFYMLTVQPSLFGEWCLVREWGRIGSPG
                     RVVSTPFPSPALAEAALDKWQRQKVRKGYSQP"
     gene            787..1170
                     /locus_tag="PLH31_10870"
     CDS             787..1170
                     /locus_tag="PLH31_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR89737.1"
                     /translation="MEGLSSPARPLLWVGSSRKDYGEFPPKVQESCGFELFLAQTGQH
                     PPSAKVLKGIGGGMVELVENFDGDTYRTVYTVRFELAVYVLHAFKKKSKHGIKTPQSD
                     IELIKRRLKDAEADYAARTKQEKEP"
     gene            1167..1511
                     /locus_tag="PLH31_10875"
     CDS             1167..1511
                     /locus_tag="PLH31_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89738.1"
                     /translation="MTAKNSVVYEGDSNIFAELGLPEADGHFLKAQIVAEIYRLTKDM
                     KLTQARAGDLMGITQPEVSRLFKGNFREYSVERLMGFLTAFAQDVEIVTRPRDHSAGR
                     GHISFTAARATA"
     gene            1745..>2371
                     /locus_tag="PLH31_10880"
     CDS             1745..>2371
                     /locus_tag="PLH31_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR89739.1"
                     /translation="MKIITLTTQKGGTGKTTLATSLAVAAMEAGETVAAFDLDPQGSL
                     ARWGELRTAETPIVETFPAKNVAQLPDMVRGLANKGFSLVIIDTPGADNTATHKAMEA
                     ASWCLVPIRPTRLDAMAVQQTVRALMRGDKPFAFVLPDRLPGRFRGRAGGNRVCAQGT
                     CRRGSPRPVGLDQSPSPGGDPMSRRPSIFDASDFATVPKKAPAAQKAKP"
BASE COUNT          470 a          788 c          685 g          418 t
ORIGIN      
        1 ggccgggtgc agccgcatct tcaaggaaaa ggtcagcggc gcccgggcag atcgccggga
       61 gctgaatcgg ctccttgccg ccctccgtga cgacgaccag gtggtggtca cccgcattga
      121 ccgcctggcg cgctccacct tcgacctgtt cgccatcgtc cggcgcatcg tggacgccaa
      181 ggcgcaattc cgctccctgg ccgagccctg ggccgacacg gccaccagca ccggccgtct
      241 gatgctcgcc gtcctcggcg ggctcgccga cgtggaacgc gacctgatcc gcacccggac
      301 ggccgagggc cgcgcccgcn nnnnnnnnng gtgatgggcc gccggtcttc cctcagcccc
      361 cagcaaaagc aggaggtgcg cgagcgcctg gcggacggcg cccagctcgc cgacctggcg
      421 cgcaccttca acgtcagccg ctccaccatc tccaggctga ggccccgccc atgagcaccg
      481 tccagctgcg cagggttgat cccagccgga acatggcccg cttctacatg ctcaccgtgc
      541 agcccagcct gttcggcgaa tggtgcctgg tgcgcgaatg gggccgcatc ggctctcccg
      601 gccgggtggt ctcgaccccc ttcccctccc cggccctggc cgaggcggcc ctggacaagt
      661 ggcagcggca gaaggtccgc aaggggtatt cccagcccta acaaggccga gcaagagcgc
      721 gggctctcct ggcctgtcag gaaacttctt gacaaaatat gagttcatac tcataataat
      781 gcgtggatgg aaggactatc gagccccgct cgtcccctgc tgtgggttgg gtccagccgc
      841 aaggactacg gggagttccc accaaaagtg caggaatcgt gcggctttga actgttcctg
      901 gctcaaaccg gacagcatcc cccatctgcc aaggtgctga aaggcatcgg tggtggtatg
      961 gttgaacttg tcgaaaattt cgacggcgac acctaccgca ccgtttacac ggttcgcttt
     1021 gaactggcgg tgtatgtgct tcacgccttc aaaaagaagt cgaagcacgg aatcaaaacc
     1081 ccacaaagcg acatcgaact catcaagcgt cgtctcaaag acgctgaggc cgattacgca
     1141 gctagaacga aacaggagaa ggaaccatga ccgccaagaa tagcgtcgtt tacgaaggcg
     1201 acagcaatat cttcgcagaa cttggccttc ccgaggcgga tggtcatttc ctgaaagctc
     1261 agattgttgc ggaaatctac cgtctaacca aagacatgaa gctcacacaa gcccgcgcgg
     1321 gcgatctgat gggcattact cagccggaag tctcccgcct cttcaagggt aacttccgag
     1381 agtattccgt ggaacgtctg atggggttcc tgacggcctt cgcgcaggat gtggaaattg
     1441 tgacccggcc ccgcgatcac agcgcgggtc gtggtcacat cagcttcact gccgctcggg
     1501 cgacggcctg agtcctcccc cgcaagggga aagcccttgc cccccaccct tccccgcgtt
     1561 cgccacatgg caggggttca ggcaggctga acccctgatc tatttaaaac agaggcttag
     1621 gctcatccgc agcgtcactg tgattttatg ctacaatgct gttatgttac agcataaata
     1681 tgctgttgtg cctctgtgat cttatgctgt agcgtcacag cgtttttatg ctattatgct
     1741 gcccatgaag atcatcaccc tcaccaccca gaagggcggg accggcaaga ccaccctggc
     1801 cacctccctt gccgtcgccg ccatggaagc cggtgagacc gtcgccgcct tcgatctcga
     1861 tccccagggc tccctggcgc gctgggggga actgcggacc gctgaaaccc caatcgtcga
     1921 gacattcccg gcaaagaacg tcgcgcagct gcccgacatg gtgcggggcc tggccaacaa
     1981 gggcttcagc ttggtcataa tcgacacgcc cggcgccgat aacaccgcca cccacaaggc
     2041 catggaagcc gccagctggt gcctggtccc catccggccg acccgccttg atgccatggc
     2101 cgtgcagcag accgtccggg cgttgatgcg cggcgacaag ccctttgcct tcgtgctccc
     2161 ggaccgacta ccaggacgct ttcgcggccg ggcagggggt aaccgagttt gcgcccaagg
     2221 gacgtgccgc cgaggaagtc cgcgccctgt gggcctggat caatcgccaa gccccggagg
     2281 tgacccaatg agccgcagac cttcaatttt cgacgcatca gactttgcaa cggtgcccaa
     2341 aaaggccccc gccgcccaaa aggcgaagcc c
//
LOCUS       DAOMEO010000475         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4793_length_2367_cov_2.295848, whole genome shotgun sequence.
ACCESSION   DAOMEO010000475 DAOMEO010000000
VERSION     DAOMEO010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_2367_cov_2.295848"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLH31_10885"
     CDS             complement(<1..908)
                     /locus_tag="PLH31_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR89740.1"
                     /translation="MTEQYDVVIVGGGHNGLIAGTYLSKAGKRVLVVERAAMAGGLSQ
                     SDHVIPQAPDHMINTGTAELIHIRASPVMQELDLRSHGWKTVETDPMYAYLDPEGGSI
                     AVFRDPRRTAEDIARFSKVDAKAYLEFVELIDGLMQFAGAMGKGDPGVRTARNYLDLA
                     KVAIRNRALKDRLQLISTAPADQLAAEWFEHPATQALLLGIVAGAGPFDTDGNGIAYA
                     LFGLLHRVGVSKAVGGMRMFADVLLSAYAASGGQLMLNAEVEEIVIGDGAARGVRLKG
                     DRMIEAGVVIATCDPITAARLATPGGL"
     gene            complement(911..1261)
                     /locus_tag="PLH31_10890"
     CDS             complement(911..1261)
                     /locus_tag="PLH31_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR89741.1"
                     /translation="MTIAKSEIERFLHGQIDAWNAHDHAAFFAFYREFAANGLTIEYV
                     GKPPRDPWEILETMWTDHNAGMRLEAVKAVINGHEAACHHRNHIEAAGVVIETMELYD
                     FGDGTLSIRYFIDA"
     gene            complement(1258..2292)
                     /locus_tag="PLH31_10895"
     CDS             complement(1258..2292)
                     /locus_tag="PLH31_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89742.1"
                     /translation="MRTFNRTMPRADFNYQVLVAPMEVSAEAMNDTRGFFGLVMNKTM
                     PYGVLRDAEGHLYAPVRALMAPTGTPNPTTFFYQSTRLGEDTLVMDKPRMAAQAQTAL
                     PVQTLEGDTVVWRSPKSDAGNAWRVTASAERFSWKEDGLFELSGKLLGVGMQWYLPGV
                     DWGTFYTAQIYEVAGQCDGHEVKGVISLDQAYLAEGGAIHYKKDLIVNNGMHVIWWNF
                     ATVYADGTFDVGSFCVGHNMLGYAIFQNEKGEIRTTTKIEGVSHHPDGSYFCDTARIV
                     LDDSEEWEFLPDPKGRMVSFLGGFPITAQQEGRWRRVGDTRQPSHWLGWGETDRRNGA
                     ARNVRGSDIA"
BASE COUNT          388 a          778 c          762 g          439 t
ORIGIN      
        1 agcccccccg gcgtcgccag ccgcgcagcg gtaatcggat cgcaggtcgc gataacgacc
       61 ccggcctcga tcatccgatc gcccttgagg cgcacgccgc gcgcggcgcc gtcgccgatc
      121 acgatttcct cgacctcggc gttcagcatc agctggccgc ccgaggcggc ataggcgctc
      181 agcaggacat ccgcgaacat ccgcatcccg cccacggcct tggacacccc gacccggtgc
      241 agcaggccaa acagggcgta ggcgatgccg ttgccgtcgg tgtcgaacgg cccggcgccc
      301 gccacgatgc ccagcagaag cgcctgtgtc gccggatgct cgaaccactc cgccgccagc
      361 tgatcggccg gagcggtgct gatcagctgg agccgatcct tcagcgcccg gtttcggatc
      421 gccaccttcg cgagatccag atagttgcgc gccgtcctga cgcccggatc gcccttgccc
      481 atcgcgccgg caaattgcat caggccgtcg atcagctcga cgaattcgag ataggccttc
      541 gcatcgacct tcgagaaccg ggcgatgtct tcggccgtcc tgcggggatc gcggaacacg
      601 gcgatcgagc cgccctctgg atcgagatac gcatacatcg gatcggtttc gaccgtcttc
      661 cagccgtggc tgcgcaggtc gagttcctgc atgacgggcg aggcgcggat atggatcaac
      721 tcggccgtgc cggtgttgat catgtgatcg ggggcctggg ggatcacatg gtccgattgc
      781 gacagcccgc cggccatcgc cgcgcgctcc acgaccagca cacgcttgcc cgccttgcta
      841 agataggtcc cagcgatcag gccgttatgg ccgccgccca cgatcacgac atcgtactgc
      901 tcggtcatcg tcaggcgtcg atgaagtagc ggatcgacag ggtcccgtcg ccgaagtcgt
      961 agagctccat cgtctcgatc acgacaccgg cggcctcgat gtggttgcga tgatggcagg
     1021 cggcttcatg gccgttgatc accgccttga ccgcctccag ccgcatgccg gcgttgtggt
     1081 cggtccacat ggtctcgagg atttcccagg gatcgcgcgg cggtttgccg acgtactcga
     1141 tggtcaggcc gttggcggcg aactcacgat agaaggcgaa gaaggccgca tgatcgtggg
     1201 cgttccaggc gtcgatctgg ccgtgcagga accgttcgat ctcggatttg gcgatcgtca
     1261 tgcgatgtcc gagccccgga cgttgcgcgc cgcgccgttg cgccggtcgg tctcgcccca
     1321 gcccagccag tgcgacggct ggcgcgtgtc gccgacgcgt cgccagcggc cttcctgctg
     1381 cgccgtgatc ggaaagccgc ccaggaagct gaccatccgg cccttcggat cgggcaggaa
     1441 ttcccattcc tcgctgtcgt cgagaacgat ccgggccgtg tcgcagaaat aggagccgtc
     1501 gggatgatgc gacacgccct cgatcttggt cgtggtgcgg atctcgccct tttcgttctg
     1561 gaagatggcg tagcccagca tgttgtggcc gacgcagaac gacccgacgt cgaacgtgcc
     1621 atccgcatag acggtggcga agttccacca gatgacatgc atgccgttgt tgacgatcag
     1681 gtccttcttg tagtggatcg ccccgccctc ggcgagatag gcttgatcca gcgagatcac
     1741 gcccttgacc tcatggccgt cgcactggcc ggccacctca tagatctggg cggtgtagaa
     1801 ggtgccccag tcgacaccgg gcagatacca ttgcatgccg acgcccagca gcttgccgct
     1861 gagctcgaac aggccgtcct ctttccagct gaaccgttcg gcggaggccg tgacccgcca
     1921 ggcgttgcct gcgtcgcttt tgggcgagcg ccagaccacg gtgtcgcctt ccagcgtctg
     1981 gacgggcagg gcggtctggg cctgggccgc catgcggggc ttgtccatga ccagcgtgtc
     2041 ttcgcccagc cgcgtggact gatagaagaa ggtggtcgga ttgggcgtgc cggtcggggc
     2101 catcagggcg cggaccgggg cgtagaggtg gccttcggcg tcccgcagca cgccgtaggg
     2161 catggtcttg ttcatcacca gcccgaagaa tccccgggtg tcgttcatcg cctcggcgct
     2221 gacctccatc ggcgcgacta gcacctgata attgaagtcg gcgcggggca ttgtcctgtt
     2281 gaacgtgcgc atgttctaaa cagtttccat atttttgttt agttaagctg gggaaagctc
     2341 agccgtccag cagggaatcc cgacgcg
//
LOCUS       DAOMEO010000476         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4795_length_2367_cov_1.981834, whole genome shotgun sequence.
ACCESSION   DAOMEO010000476 DAOMEO010000000
VERSION     DAOMEO010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_2367_cov_1.981834"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLH31_10900"
     CDS             complement(<1..317)
                     /locus_tag="PLH31_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HQR89743.1"
                     /translation="MAAAIGAWGGGQYVVRRMHHATPLHQLVHEQLDLTPDQERRIDG
                     LERQYAAKRLTLEAEMRAANAELAQAIQEQHSYTPKVQAAIDRFHMAMGALQKESVQH
                     VIA"
     gene            complement(359..637)
                     /locus_tag="PLH31_10905"
     CDS             complement(359..637)
                     /locus_tag="PLH31_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89744.1"
                     /translation="MTLNIDDLIDRLAARPLDRSLDGLEWDVTRGIARRSAELKAGAA
                     LAPVRVASAMGVTAGGLAAAGTVAAPQQLNTFSVAAHLAPSTLLEGER"
     gene            complement(771..1061)
                     /locus_tag="PLH31_10910"
     CDS             complement(771..1061)
                     /locus_tag="PLH31_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQR89745.1"
                     /translation="MNMSKTDMKAHAQMGRDALKMDEMRMSKAMMAVEASDFDVLWAK
                     KMIVHHQGAIEMSRSLLKHAHDAEAKRMAQATIDENTKAKAELEMFIRKHGG"
     gene            complement(1492..1836)
                     /locus_tag="PLH31_10915"
     CDS             complement(1492..1836)
                     /locus_tag="PLH31_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HQR89746.1"
                     /translation="MKTLLAAAALALALTGPALAQSDMKMGDMPGMTHDAMPAAAKTV
                     DGQGVIKGLDKANGVVTLQHQPIPALKWPAMTMKFKADPAVLKPFKVGQAVNFKLKPT
                     GGSTEIVAMSAK"
     gene            complement(1939..>2367)
                     /locus_tag="PLH31_10920"
     CDS             complement(1939..>2367)
                     /locus_tag="PLH31_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89747.1"
                     /translation="LPFALTGGFWAIYLLGFNQSVATGVGFIALAGVSAEFGVVMLIY
                     LKQALAERRGPIKEADLVQAIRDGALLRVRPKAMTVAVILAGLMPILVGHGAGSEVMS
                     RIAAPMIGGMLSAPLLSMFVIPAAYLLIQRHRRRAARPRS"
BASE COUNT          438 a          759 c          712 g          458 t
ORIGIN      
        1 atggcgatca catgctggac gctttctttc tgaagcgcgc ccattgccat gtgaaaacga
       61 tcaatcgccg cctgcacctt gggggtatag ctgtgctgtt cctggatggc ctgggcaagt
      121 tcagcgtttg cggcccgcat ctcagcttcc agcgtcaggc gtttggctgc gtactgacgc
      181 tccaagccat caatgcggcg ctcctggtcg ggcgtcagat caagttgttc gtgcacgagc
      241 tgatgcagcg gcgtcgcatg atgcatccgg cgcacgacat attgcccgcc gccccaagcc
      301 ccgatcgcgg cggccaaggt cgacaagacc agtgtcagga cgatgctgcg actgatgctc
      361 accgttcgcc ctctaacaat gtcgatggtg cgagatgcgc agcgaccgaa aaggtgttga
      421 gttgctgcgg cgcggccacc gtgccggcgg cggccaagcc gccagctgtg acacccatgg
      481 ccgaggcgac gcggacggga gccaatgctg cgccggcctt cagctcagca ctgcggcggg
      541 cgatgccgcg cgttacgtcc cattctagcc cgtccaacga ccggtcaagc ggtctagccg
      601 ccaaccggtc gatgaggtca tcgatgttca aagtcatgtt cggtccttcg ccccgaggtg
      661 tcccgctgac tatacgcggc agagaccctt tatcccgcaa gtgcggaccc cgcccgaaaa
      721 tgacgctcgg cgccatgccc gccggcgcca gtttcagata ggctgcggcg ttatccgccg
      781 tgcttgcgga taaacatctc cagctcggct ttggccttcg tgttttcgtc gatcgtcgcc
      841 tgcgccatac gtttggcttc cgcgtcgtgg gcatgcttga gcagtgatcg gctcatctcg
      901 atggcgcctt gatggtggac gatcatcttc ttggcccaca gcacgtcaaa gtcggaggcc
      961 tcaacggcca tcatcgcttt gctcatccgc atctcatcca tcttcagggc gtcgcgaccc
     1021 atctgggcgt gggccttcat gtcagtcttg gacatgttca tgcccttcat gtcatgcccg
     1081 gccatcgact gagccgaagc cccggtggcg ccggccgcag ccagcaggct cccggcgcaa
     1141 atcgcggtca aaatcaaacg catctcgaat ttccttcaga ctgcttggcg aacctgcgcg
     1201 tcacaattga cgcgatccgc ccgaccaaac agtcctcgca acctattcgt tgcgttacgg
     1261 gtgcatatat ttcaaacgca cgacaagtat tacagcgcag acgccttttt attggtggta
     1321 tttttactct gcgataactt ccccaatgca atggcgaccc cccggtatgg caagtttggg
     1381 atagactagc gcttagtctg gaaaccctag atcgaacgtc agggaaggcc aacgtcgagc
     1441 caaggactag gccgttggtt ggccgcacgc ggcggccaac cgtctgaagg gctactttgc
     1501 cgacatcgcc acgatctctg tcgaaccgcc cgttggcttg agtttgaaat tgacggcctg
     1561 tccgaccttg aacggcttga gaacggccgg atcggccttg aacttcatgg tcatggccgg
     1621 ccattttagg gcgggaatgg gctggtgctg cagcgtcacc acgccgttgg ccttatccag
     1681 acccttgatc acgccttggc catccacggt cttggccgca gcgggcatag cgtcgtgcgt
     1741 catgcccggc atgtcgccca tcttcatgtc cgattgagcc aaggcggggc cagttagggc
     1801 cagggccaaa gcggctgcgg ctagcaaggt cttcatcgat ggtcgctcct gtcaggcgga
     1861 cgggtcaggg agaacccttc acagcggagg atatacgcgc cgccccgccg acacccctca
     1921 ggcgaagcga tcgcaccgtc acgaccgggg tcgcgcggca cgccttcgat ggcgctggat
     1981 caacagatag gcagcgggaa ttacgaacat cgacagcagc ggcgcgctga gcatgccgcc
     2041 gatcatcggc gcggcaatgc ggctcatcac ttcggagcca gcgccgtggc cgaccaggat
     2101 cggcatcagt ccggccagga tcaccgccac ggtcatggcc ttgggccgca cgcgcagaag
     2161 cgccccgtcg cggatggcct ggacgaggtc ggcctccttg atcgggccgc ggcgctcggc
     2221 cagggcctgc ttgagataga tcagcatcac caccccgaac tcggccgaga ccccggccaa
     2281 ggcgatgaag ccgacgccag ttgccaccga ctggttgaag cccagcaggt aaatcgccca
     2341 gaagccgccg gtcagggcga agggcag
//
LOCUS       DAOMEO010000477         2364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4802_length_2364_cov_2.952360, whole genome shotgun sequence.
ACCESSION   DAOMEO010000477 DAOMEO010000000
VERSION     DAOMEO010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4802_length_2364_cov_2.952360"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1137
                     /locus_tag="PLH31_10925"
     CDS             103..1137
                     /locus_tag="PLH31_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR89748.1"
                     /translation="MRTESGARIAVAGAGVFGLASALALARAGLAVTVFDPARPGDNA
                     SGVAAGMLAPASEALFDATSTPHLALLRRARDLWPTFVEGLDVEILQADVRIEGEADW
                     LCQIDARRQVLGLPQRREPDGALILNEDWRLDACSTLAALRTAASALGVVFEAASVTH
                     FEPGALTLSDGRVEGFDVLVLATGASQRDGALAPETASLAPIKGQILQLDPGQAAGSV
                     VRGSGVYLCPGAPMKVGATMEFGRDDLSPDPAITNPLLQAAIALRPALAKATVAIEVG
                     VRASTPDGLPLVGWSARAGILLAVGARRNGWLLAPLVAGLVAAYLTDKDPGQDGAQLD
                     ARRFDQELEA"
     gene            1154..2317
                     /locus_tag="PLH31_10930"
     CDS             1154..2317
                     /locus_tag="PLH31_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR89749.1"
                     /translation="MSGFWLTEEQEAIREGVAKLCAGFDDDYWRRTDETGNFPEEFVA
                     AIAEGGWLGVAMPESVGGAGLGLTEASVMMQAVAESGAGFSGASAIHLNIFGPMPIVK
                     FGSDEQRARHLPRLISGEDKMCFAVTEPNSGLDTSSLETRAEKVDGGYRLNGRKIWTT
                     GAQRANKILIIARTTPKDQCAKPTQGLSLFYTDREKIEAKPIPKMGRKAVECNMLFIE
                     DLFVPQEDLVGEEGKGFAYLLHGLNPERVLFAVEAIGLGRAALAKATTYAKERVVFGR
                     PIGQNQGVAHPLARSWAELEAANLLAFKAAALYDMGKDCGAEANASKYLGAEAGFTAC
                     ENAVLAHGGMGYAKEYDVERYFREAMIARIAPISREMILNFIAERVLGLPKSY"
BASE COUNT          370 a          749 c          832 g          413 t
ORIGIN      
        1 cccactgcgt caatgcgcgc cagggtcgag cctgtcggcc ctgttgcgtg aaactgacct
       61 gaagggcttg acctttggcc tatcggcggg tgtttgcgcc gcatgagaac tgagtcaggc
      121 gcgcgcatcg cggtggccgg cgccggcgtt tttggcctgg ccagcgcctt ggccctggcg
      181 cgcgccggcc tggccgtgac ggtcttcgat ccggccaggc caggcgacaa cgcgtcgggc
      241 gtcgcggccg gcatgctggc gcctgcgagt gaagccctgt tcgatgcgac ctcgacgccc
      301 catcttgctc ttcttcggcg ggcgcgggac ctgtggccga ccttcgtcga aggtctggat
      361 gtcgaaatcc tgcaggctga cgtgcgcatc gagggtgagg ctgactggct ctgccagatc
      421 gatgcgcggc gacaggttct gggtctgccg cagcgtcgcg agccggacgg agccctgatc
      481 ctcaacgagg actggcggct ggacgcctgc agcaccctag cggctcttcg aacggcggca
      541 agcgccctcg gcgttgtttt cgaggcggct tcggtcaccc acttcgagcc cggcgcgctg
      601 acgctgtccg atggacgggt cgagggcttc gatgtccttg tgctggccac gggagcctcc
      661 cagcgcgacg gggcgctggc gccggagacc gcctccctgg cgccgattaa ggggcagatc
      721 ctgcagctgg atccggggca ggccgcaggt tcggtcgtgc gcggctcggg cgtctatctg
      781 tgtcccggag cgccgatgaa ggtgggggcg acgatggagt ttggtcgaga cgatctgtct
      841 cctgaccccg cgataacaaa tccgctgctc caggcggcga tagccctgcg gcccgctctt
      901 gcgaaggcga ccgtggcgat tgaggtcggc gtgcgcgcct ctacccccga tggccttccg
      961 ctggtcggct ggagcgcccg ggccgggatc ctgcttgcgg tcggcgcccg acgcaacgga
     1021 tggctgctgg ccccgcttgt cgcgggtctg gtcgcggcct atttgacaga caaggacccg
     1081 ggccaggatg gggcgcagct tgatgcgcgc cgtttcgatc aggagctcga ggcttgaaag
     1141 cgcaggggaa gagatgtcgg gattttggct gaccgaagaa caggaagcga tccgcgaagg
     1201 cgtagccaag ctatgcgccg gcttcgatga tgactactgg cgccgcaccg acgagaccgg
     1261 aaactttccc gaggagtttg tcgccgccat cgccgagggc ggttggctgg gtgtggcgat
     1321 gcctgaaagt gtcggcggcg ccggcctggg cctgaccgag gcgtcggtga tgatgcaggc
     1381 ggtggcggag tcgggcgctg gcttctcggg cgcgtcggcc atccacctga acatttttgg
     1441 tcccatgccg atcgtcaagt tcggttccga cgagcagcgc gccaggcacc tgccgcgcct
     1501 gatctcgggc gaggacaaga tgtgctttgc ggtgactgaa cccaattcgg gcctggacac
     1561 ctccagcctg gagacccgcg cggaaaaggt cgatggcggc tatcgcctca atggccggaa
     1621 aatctggacc accggcgccc agcgcgccaa caagatcctg atcatcgccc ggaccacgcc
     1681 gaaggatcag tgcgccaagc cgacccaggg cctgagcctg ttctataccg accgcgagaa
     1741 gatcgaggcc aagccgatcc cgaagatggg tcgcaaggcc gtcgaatgta acatgctctt
     1801 catcgaggac ctgttcgtgc cccaggagga tctggtgggc gaggagggca agggctttgc
     1861 ctatctgctg catggtctga atcccgagcg ggtgctgttc gccgtggagg cgatcggcct
     1921 ggggcgcgcc gctctggcca aggccacgac ctacgccaag gagcgcgtgg tgttcggtcg
     1981 gcccatcggc cagaaccagg gcgtcgccca tcccctggcc cggtcatggg ccgagctgga
     2041 ggccgccaac ctgctcgcct tcaaggcggc ggcgctctac gacatgggca aggactgcgg
     2101 ggccgaggcg aacgcctcca agtatctggg cgccgaagcc ggcttcaccg cctgcgagaa
     2161 cgctgtcctg gctcatggcg gcatgggcta tgccaaggaa tacgatgtcg agcgctactt
     2221 ccgcgaagcc atgatcgctc gcatcgcgcc gatcagccgc gagatgatcc tcaacttcat
     2281 cgccgagcgc gtgctgggcc tgcccaagag ctactagctg gaaggccggg tctgtcagca
     2341 ggcccggcca ttttgtctga cgcc
//
LOCUS       DAOMEO010000478         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4809_length_2362_cov_2.511487, whole genome shotgun sequence.
ACCESSION   DAOMEO010000478 DAOMEO010000000
VERSION     DAOMEO010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_2362_cov_2.511487"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLH31_10935"
     CDS             <1..355
                     /locus_tag="PLH31_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89750.1"
                     /translation="GEVDIEARLLLGRSAVTRRGEIFSDLQLARLRRSGLADETRMDA
                     TLGFRPARNWLVMAQTYAGEADSKPRKARWLKAELSIVRGFGPWSVQAGWRETVAGRE
                     IAKDRGMVLGVWRTF"
     gene            478..1599
                     /locus_tag="PLH31_10940"
     CDS             478..1599
                     /locus_tag="PLH31_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR89751.1"
                     /translation="MIRRHFFLLAALAAVVLMLVVGGMKLAFGQKAAGQGGPGGGGRA
                     INVSQTVVSTRPFTDRIEVLGVAKGRQSVTITSNTAEMITAVHFTDGQAVARGQVLVD
                     LKSASEDAGVSEARVRLAQAERDYQRWKTLADRGVAPRATAEQYLAAVETARASVASA
                     QAQKLDRVIRAPFAGRVGISDVAPGALISPGTAIVSLDDVSVIRIDFSVPDRYLTTLR
                     EGLLITARPDALPGETFQGRIAQIDTRIDATTRAVKARAEFPNPGARLKPGMLVKVAI
                     DQGLRQAPAVPESAIQFEGVQASVFLIAKGPKGQVARRTNVETGLTAGGFVEILSGLK
                     SGDRIVADGLNRVQDGAAIGGGAPGGKGPGQPGAGGKAR"
     gene            1599..>2362
                     /locus_tag="PLH31_10945"
     CDS             1599..>2362
                     /locus_tag="PLH31_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89752.1"
                     /translation="MLSDLSVRRPVFAAVAAIILCVIGLAAFTGLPIRELPNVDPAVV
                     SVSTAYRGASAEVIEERITQVIERQVSGIQGIDRVNSSSRDGRSQINITFTLDRDLDD
                     AANDVRDAVSRVSANLPDQADPPQIAKANADSSPIVIMNLTSTTLSPLELADYADRYL
                     VERMSTVPGVAQAGLNGQLYAMRIWLKPDAMAARGVTVDDVENALNSQNVELPAGALE
                     SDSKDFTIRVTRSYSKPDDFLKLPLRAADANGFVLRL"
BASE COUNT          361 a          784 c          786 g          431 t
ORIGIN      
        1 gggcgaggtc gatatcgagg cacggctgct tctgggccgg tcagccgtga cccggcgcgg
       61 ggaaatcttt tcggatctgc aactggcccg tttgcgccga tctggcctgg ctgacgagac
      121 ccggatggat gcgacattgg gctttcgccc cgcgcgcaac tggctggtga tggcgcagac
      181 ctatgctggc gaggccgact cgaagccccg aaaagcccgc tggctcaagg ctgagctatc
      241 gattgtccgc ggctttggtc cctggagcgt tcaggcggga tggcgggaga ctgtggccgg
      301 tcgcgagatc gccaaggatc gcggcatggt gctcggggtg tggcgcacct tctgagtgtt
      361 gcagcgctga aacggtccgt catcttgcgg ttactggacg cgggtctagg gggggcggca
      421 tatgtcctgg caccttgtcc cgtccgccgg ctcccctcgg cgattggagt cctttcgttg
      481 atccgcaggc atttcttcct tttggcggcg ttggccgctg tcgttctgat gctcgtcgtg
      541 ggcggcatga agctggcctt tggtcaaaag gctgcggggc aaggcgggcc cggcggcggt
      601 ggtcgggcca tcaacgtctc ccagaccgtc gtgtcgacca ggcccttcac cgatcgcatc
      661 gaggtgctgg gcgtggccaa gggccgccag tcggtgacca tcacctccaa cacggccgag
      721 atgatcacgg cggtgcactt caccgatggt caggctgttg cgcgcggcca ggtgctggtt
      781 gacctgaagt ccgccagcga agatgccggc gtcagcgagg ctagggtgcg tctcgcccag
      841 gccgagcgcg actatcagcg ctggaaaacc ctggcggatc gtggcgtcgc gccgcgcgcc
      901 accgcagagc agtatctggc cgccgtcgag accgcgcgcg ccagcgtcgc ctcggcccag
      961 gcccagaagc tggatcgcgt gatccgggcg ccctttgccg gccgagtggg tatttcggat
     1021 gtggcccccg gcgccttgat cagtcctggc acggcgatcg tcagccttga cgacgtatct
     1081 gtgatccgca tcgatttttc cgtgcctgac cgctatctca cgaccctacg tgaaggtctg
     1141 ctcatcacgg cccggcccga cgccctgccg ggcgagacct ttcaggggcg catcgcccag
     1201 atcgacaccc ggatcgacgc cacaacccgc gccgtgaagg cccgggccga gttccccaat
     1261 ccgggcgctc gtttgaagcc gggaatgctg gtcaaggtgg ccatcgacca gggtttgcgc
     1321 caggcgccgg ccgtgccgga atcggccatc cagttcgagg gtgtgcaggc ctcggtgttc
     1381 ctgatcgcca agggcccgaa agggcaggtg gcgcgacgaa ccaatgtcga gaccggtctc
     1441 accgccggcg gcttcgtcga gattctgtcg ggtttgaagt cgggtgaccg gatcgtcgcc
     1501 gacggtctca atcgcgttca ggacggcgcc gccatcggcg gcggagcgcc cggcggcaag
     1561 ggaccgggcc agcccggcgc cggcggcaag gcccgctgat gctttccgat ctttccgtcc
     1621 gccgcccggt cttcgccgcc gtcgcggcga tcatcctctg cgtcatcggc ctggccgcct
     1681 tcaccggcct gccgatccgc gaactgccca atgtcgatcc ggcggtggtc tcggtctcga
     1741 ccgcctatcg cggcgcctcg gcggaagtca tcgaggagcg cattactcag gtgatcgagc
     1801 gccaggtctc gggcatccag ggtattgacc gggtcaattc gtcgtcgcgc gacggccgct
     1861 cgcagatcaa tatcaccttc acgctggatc gcgatctgga cgacgcggcc aatgacgtgc
     1921 gcgacgcggt gagccgggtg tcggccaatc ttcccgatca ggccgacccg ccgcagatcg
     1981 ccaaggccaa cgccgacagt tcgcctatcg tgatcatgaa cctgacctcg acgaccctgt
     2041 cgccgctgga actggccgac tatgccgacc gctatctggt cgaacgcatg tcgactgtgc
     2101 caggcgtcgc ccaggcggga ctgaacggcc agctctacgc catgcgtatc tggctcaagc
     2161 ccgacgccat ggctgcgcgc ggcgtgaccg ttgatgatgt cgagaacgcc ctcaacagcc
     2221 agaatgtcga actgcccgcc ggcgccctgg agagcgactc caaggacttc accatccggg
     2281 tcacccgcag ctattccaag cccgatgact ttctcaaact gccgctgcgc gctgccgacg
     2341 ccaacggctt tgtgctgcgt ct
//
LOCUS       DAOMEO010000479         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4814_length_2360_cov_1.931453, whole genome shotgun sequence.
ACCESSION   DAOMEO010000479 DAOMEO010000000
VERSION     DAOMEO010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4814_length_2360_cov_1.931453"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>335
                     /locus_tag="PLH31_10950"
     CDS             <1..>335
                     /locus_tag="PLH31_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQR89753.1"
                     /translation="RIGRSDLGARLLSTDDPEEARMLAEELDALNTERKAVEAGVVEE
                     AAAVLERGSNFNPDAPVIVVAGEGWHPGVIGIVAGRLRERYRKPVVVVGIDRAANVGK
                     GSGRSQPGV"
     assembly_gap    336..345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            346..757
                     /locus_tag="PLH31_10955"
                     /pseudo
     CDS             346..757
                     /locus_tag="PLH31_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516823.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
     assembly_gap    758..767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <768..866
                     /locus_tag="PLH31_10960"
     CDS             <768..866
                     /locus_tag="PLH31_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89754.1"
                     /translation="ALHVAGKLKPDDYNGRNGVQLEIEDAADPRAC"
     gene            985..1059
                     /locus_tag="PLH31_10965"
     tRNA            985..1059
                     /locus_tag="PLH31_10965"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1018..1020,aa:Glu,seq:ttc)
     gene            1256..1876
                     /locus_tag="PLH31_10970"
     CDS             1256..1876
                     /locus_tag="PLH31_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQR89755.1"
                     /translation="MSGYDFEAAGAHEDFVRISGRVKWFDVGKGYGFIVPDDPGQTGL
                     KDVLLHVTSLRNCGRETAPEGAVIVCDVVRRPKGWQVSEVVDLDETSAPSPLERPRRT
                     LVDPGQRRERDGLGNGFRSGLDSAGGRQTLTPEGPAEHAKVKWFNRTKGYGFVVRDAE
                     PGDIFVHIETLRRGGLEDLQPGDDVLVRFAKGPKGLVVAEITPGDA"
     gene            1968..>2360
                     /locus_tag="PLH31_10975"
     CDS             1968..>2360
                     /locus_tag="PLH31_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQR89756.1"
                     /translation="MIGQLGRRAVLSAVLAGALFVGGIAIAKPAGKLETVELITAKGR
                     ARFQVEIAATRAEQQRGLMFRKSLAPDRGMLFTYAKPQPAAFWMKNTLIPLDIVYIAP
                     NGRVLSIVRNAQPHNEMPMPSGGPVLGVL"
BASE COUNT          382 a          724 c          792 g          442 t
ORIGIN      
        1 cgcatcggtc gctccgacct tggcgcgcgt cttctgtcca cggacgatcc cgaagaagcc
       61 cggatgctgg ccgaggaact cgacgccctc aataccgagc gcaaggccgt ggaggccggc
      121 gtggtcgagg aggctgcggc cgtgctggag cgtggcagca acttcaatcc cgacgccccg
      181 gtgatcgtgg tggcggggga gggatggcac cctggcgtga tcggcattgt cgccggccgc
      241 ctgcgcgagc gctatcgcaa gcccgtggtg gtggtcggca tcgaccgcgc cgccaatgtc
      301 ggcaagggct cggggcgttc ccagcccggc gtcaannnnn nnnnngtcaa tctgggcgcg
      361 gccatccagg cggccttcga gcagggcctg ctgatgtccg gcggtggcca cgccatggcg
      421 gcaggtctgt ctattcgccc cgactcgatc cccgagctgc gcgccttcct ggaagagcgt
      481 ctggccggcg agatggaggc ggtaggtccc gaggccgtcg agatcgacgc cctggttcag
      541 ccgcgcggcg tcgatcgcgc gcactatgaa gacttccagc gcctggcgcc gttcggtcct
      601 ggcaaccccg agccaatgtt cgccctgacc ggcgttcgcg ctgaccgcgt gatggccctg
      661 aagggcggcc acgtgaagct ggatctggtg ggtccgacgg gcgagcgcct gaaggcgatt
      721 tcctggcgca gcgccgaaac cgacctgggc cgccggcnnn nnnnnnngcg ctgcacgtcg
      781 ccggcaagtt gaagcctgac gactataacg gccgcaacgg cgttcagctg gagatcgagg
      841 acgccgccga cccgcgcgcc tgttgatctg gaacggcttt tcgcagggtg ttggcccggt
      901 ctgtgggcga acggaaaaaa tgcttggctg cgggttgcgc accccaaacg gcgcggctat
      961 acacccgctt cctcgcgggg ccgtggtccc ttcgtctatc ggttaggacg ccagattttc
     1021 attctggaga gaggggttcg actcccctag ggactaccac cctcggctgc acttttggcg
     1081 cctgcggctg tatttcagaa ttatttccga aaaatatctg cgacgaaatg tcgcggtgtg
     1141 ctcgcgagcg tgtgcttgac gcgaattcac cattgacggc tctcctaacg cgagaacagt
     1201 gttattcttg tggcgctcgt cccattgggc tgattgggcg ggccagaggg gcggaatgtc
     1261 tggttacgat ttcgaggccg cgggggcgca tgaagatttc gtgcggatca gcggacgcgt
     1321 taaatggttc gatgtcggca aggggtatgg gttcatcgtt ccggatgatc ctggacaaac
     1381 cggcctgaag gacgtgcttc tccacgtgac ctcgctacgc aattgcgggc gggaaacagc
     1441 gcctgagggg gctgtgatcg tctgtgatgt cgtgcgccgc cccaagggct ggcaggtcag
     1501 cgaagtcgtt gatctggacg agacatccgc gccctcgcca ctggaacggc cccgccgcac
     1561 cttagtcgat ccgggacaac gtcgagagcg cgacggactt ggtaatggtt tccgctctgg
     1621 tctggacagc gccggcgggc gacagactct gacgccagag gggccggccg agcatgccaa
     1681 ggtcaagtgg ttcaatcgca ccaagggcta cggcttcgtg gtccgggatg ccgagccagg
     1741 cgatatcttc gtccatatcg agaccctgcg ccgaggtggc ctggaagatc tccagccggg
     1801 cgatgacgtc ctggtgcgtt tcgccaaggg gcccaagggt ctggtggtcg ccgagatcac
     1861 tccgggcgac gcctgacttt cgccatctcg ccctgccatt ccatttactg gaccgctgcg
     1921 cctggctgcg cgtctggtcg tatggcgagt gaggacatgg cgtggcgatg atcggtcaac
     1981 tgggaaggcg agcggttctg tcggccgtgc ttgccggcgc gctcttcgtg ggcggtatcg
     2041 ccatcgccaa gccggcgggc aaactggaga ctgtcgagct cattaccgcc aagggccgcg
     2101 cgcgctttca ggtcgagatc gccgcgactc gcgccgaaca gcagcgcggc ctgatgttcc
     2161 gcaagtcgct ggcgcccgac cgcggtatgc ttttcaccta cgccaagcca cagccggccg
     2221 cgttctggat gaagaacacc ctgataccgc tcgacattgt ctatatcgcg ccaaatggcc
     2281 gggtgctgtc gattgttcga aacgcccaac cccacaacga aatgcccatg ccttcgggcg
     2341 gtccagttct cggggttctc
//
LOCUS       DAOMEO010000480         2357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4821_length_2357_cov_2.597307, whole genome shotgun sequence.
ACCESSION   DAOMEO010000480 DAOMEO010000000
VERSION     DAOMEO010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_2357_cov_2.597307"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLH31_10980"
     CDS             <1..234
                     /locus_tag="PLH31_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HQR89757.1"
                     /translation="KAVTLVLAKADFDKNGRAAPFRANDELIALEPVCVLTLANTLNG
                     ARGASLQPFGPALNLGQKAAERGIRHLPVKTRG"
     gene            427..804
                     /gene="umuD"
                     /locus_tag="PLH31_10985"
     CDS             427..804
                     /gene="umuD"
                     /locus_tag="PLH31_10985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HQR89758.1"
                     /translation="MGFRICCGFPSPADDWIEDAIDVSRLVVTNPAATFLWRASGDCM
                     TPTILDGDYLVCDRSLAPVSGDVVVAVVDGEPTVKRLAYVAGRPTLSHDNPLSPPFAP
                     DPHSEISIWGVVAWSLRAHRPTR"
     gene            801..>1579
                     /locus_tag="PLH31_10990"
     CDS             801..>1579
                     /locus_tag="PLH31_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HQR89759.1"
                     /translation="MRPVIALSDGNCFYVSCERVFDPRLEGRPTVVLSSNDGNIIARS
                     PEAKAIGLKMGDPYFKVRELCARERVAALSSNYVLYGDMSRRVNAVYERFAPEVVVYS
                     IDESFLDFSGLADPVGHARALRATVRQWTGIPTCVGLGPTKTLAKVANHLAKRTPALD
                     GVCDLSDPQVRAAALDDLPVGDVWGVGPAYAARLTAMGVTTAGQLRDLDPRQARAVLS
                     VVGERLVLELRAQTCLSWDDVPAGRKGCAVTRSFGQPITDLG"
     assembly_gap    1580..1589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1590..2072
                     /locus_tag="PLH31_10995"
     CDS             <1590..2072
                     /locus_tag="PLH31_10995"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HQR89760.1"
                     /translation="DLLQAVASFASRVGEKLRRNGVVAPRLSVFMHTNPFGAGPHHSA
                     SGAVTFLEATADSTELVAGARQAARQAWRDGYRYSKAGVLCEGLVPAGQVQASLLAAK
                     DPARSERLMAALDASNRRFGRGSVVIGATTGRTRWTQKAEWRTPRYTTRLDEVPVARA
                     "
BASE COUNT          336 a          824 c          792 g          395 t
ORIGIN      
        1 aaggcggtca ccctggtgct ggccaaggcc gacttcgaca agaacggccg cgccgccccc
       61 ttccgggcca atgatgaatt gatcgccctc gaaccggtct gtgtcctgac cctggcgaac
      121 accctgaacg gcgcgcgcgg ggcgtcactg cagcccttcg gacccgccct caatctcggt
      181 caaaaggccg ctgaacgagg catccgccac cttccagtga agacacgcgg gtgaccccgc
      241 agggcgtggc cgctggcgcg gtcacgccca tttgtctgtt ggcggtctgt ggacaggcgc
      301 gatgccccct tgccggggtg caaatgttct acttatgttc tcgacctcgc cgcctgcccc
      361 gtgtcggcag gctttcgagg tctcatgcgt cccgttgtca tcggtcttcc ccttcccctc
      421 cccctcgtgg gctttcgcat ttgctgcgga ttccccagcc cggccgacga ctggatcgaa
      481 gacgccatcg atgtcagccg gctcgtcgtc accaaccccg ccgccacctt tctgtggcgt
      541 gcgtccggag actgcatgac gccgaccatc ctggacggcg actacctggt ctgcgaccgc
      601 tcgctggccc ctgtctccgg cgacgtggtg gtcgccgtcg tcgacggcga gccgacggtc
      661 aagcgcctcg cctatgtcgc cggccggccg accctcagcc atgacaatcc cctgtcgcca
      721 cccttcgcgc cggacccgca cagtgaaatc agcatctggg gcgtggtcgc ttggtccctg
      781 cgcgcgcatc ggccgacccg atgaggccgg tgatcgcgct gagcgacggc aattgcttct
      841 acgtctcgtg cgagcgggtc ttcgatcccc gcctggaagg acgaccgacc gtggtgctgt
      901 cgtccaatga cggcaacatc atcgcgcgca gccccgaggc caaggcgatc ggcctcaaga
      961 tgggcgatcc ctatttcaag gtccgcgagc tttgcgcccg ggagcgggtc gccgccctgt
     1021 cgtcgaacta cgttctctat ggcgacatga gccggcgcgt gaacgccgtc tatgagcgct
     1081 tcgcgcccga ggtcgtcgtc tacagcattg acgagagctt cctcgatttc agcggtctcg
     1141 ccgatccggt cggtcacgcc cgcgccttgc gcgcgacggt ccgccagtgg accgggattc
     1201 cgacatgtgt cgggcttggt cctaccaaga ccctggccaa ggtggctaac cacctggcca
     1261 agcgcacgcc ggcgctggac ggcgtttgcg atctgagcga cccgcaggtg cgcgccgcgg
     1321 ccctggacga tctgcccgtt ggcgacgtct ggggcgtggg cccggcctat gccgctcggc
     1381 tgacggccat gggggtgacc acggccggcc aactgcggga cctcgatccg cgtcaggccc
     1441 gagccgtgct cagcgttgtc ggcgagcgcc tggtgctgga gctgcgcgcc cagacctgtc
     1501 tgtcgtggga cgatgttccg gcaggccgca aggggtgcgc ggtgacccga agctttggcc
     1561 agccgatcac ggacttaggn nnnnnnnnng acttgctgca ggcggtcgcg agcttcgcca
     1621 gccgggtagg tgagaagttg cgccgcaatg gcgttgtcgc cccgcggctg tcggtgttca
     1681 tgcacaccaa tccctttggc gcggggccgc atcacagcgc cagtggcgcc gtcaccttcc
     1741 tggaagccac ggccgacagc accgagctcg tcgccggcgc gcggcaggcg gcgcgccagg
     1801 cctggcggga cgggtatcgc tacagcaagg ccggcgtcct ctgcgagggc ctggttcccg
     1861 caggtcaggt gcaggcaagc ctgctggcgg cgaaggatcc ggcgcgatca gagcggctga
     1921 tggcggcgct ggacgcctcg aaccgccggt ttgggcgcgg cagcgtggtg atcggggcga
     1981 ccacgggccg gacgcgctgg acgcagaaag ccgagtggcg gacgccgcgc tatacgacgc
     2041 gcctggacga ggtgcctgtc gcccgggcct agggcgctcc aacggcaaga tggcagcggc
     2101 ctcgaggggg tgtgatcctc gcacgtcacc gcaacatcga aggtcgtctc ccagatgggc
     2161 gccgccgaag cgggctcgtc cgcgatccag ctcacgacca tagggcgggc cggttagggg
     2221 ccaaggcgac ctcgccgaag gcgaggcggg gccgacggac cctcacggcc ttcgctgcgc
     2281 tgcaggccgg tttccatcgg ccccgaccct gtcccctgcc ctattccccg cccttcccgg
     2341 tcgcgagatc gcgggaa
//
LOCUS       DAOMEO010000481         2354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4830_length_2354_cov_3.122662, whole genome shotgun sequence.
ACCESSION   DAOMEO010000481 DAOMEO010000000
VERSION     DAOMEO010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4830_length_2354_cov_3.122662"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..960)
                     /locus_tag="PLH31_11000"
     CDS             complement(19..960)
                     /locus_tag="PLH31_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HQR89761.1"
                     /translation="MTHACAAGVDVGRDWLDVGLAPSGKVFREANAPSGIVALVARLK
                     REGAGKVVLESIGGYGARLVRSLAEAGFEVGVIDPKRIRALRVAEGRRRAKTDRLDAG
                     LIARFALLMQDAARPVPDAKAFEIRALSTRRRQLVEMAAMEKVRLKQTLDEAVADSCR
                     QMIAWLSQERANIEAQLELMLLETQAGQARRGLLQTIPGVGPAVSMTLLADLPELGSL
                     DRKAVGSLAGLAPHPNQSGTRTGAAQIGGGRPCVRAALYMAAVSATRTDKGFKREYLA
                     MRQAGKPAKVALVAIARKIVVAANSMLKSGQPWQKPA"
     gene            1131..1214
                     /locus_tag="PLH31_11005"
                     /pseudo
     CDS             1131..1214
                     /locus_tag="PLH31_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1246..2151
                     /locus_tag="PLH31_11010"
     CDS             1246..2151
                     /locus_tag="PLH31_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQR89762.1"
                     /translation="MALRKHLALAALGAVLGAILLTTAPGVAAEEQPWRLVWAEEFDG
                     DRLDETRWTHAADCGGGGNNERQCYVVSPDTVSVRDGLLRLTAIKRKTRGLANPWAGP
                     TGPMKTGDYASGKIVSQGKASWLYGRIEARARVPGGQGVWPAIWMMPETNAYGGWPRS
                     GEIDILETINLGAPCDTCEGGRENRTFGTIHFGKGPGADHRQLGGHTVSAPTADDFHV
                     YAVEWSAGKIDWFVDGVRYFTATAEDWKPADGEASPAPFDQPFHLILNLAFGGAWPEG
                     ANARGVDEAALPATMEVDWVRVSQR"
BASE COUNT          366 a          818 c          810 g          360 t
ORIGIN      
        1 gccggcgact gtgttggatc aagcgggttt ttgccaaggc tgtcccgact tgagcatgct
       61 gtttgcggcg acgacgatct tcctggcgat ggcgacgagg gcgaccttgg caggcttccc
      121 ggcctgccgc atggccaggt actcgcgctt gaagcctttg tcggtgcggg tggcgctgac
      181 ggcggccatg tagagggcgg cgcgcacgca aggtcgtccc ccgccgatct gggccgcgcc
      241 cgtccgcgtt ccgctctggt tgggatgagg cgccaggccc gccagactgc cgacagcctt
      301 gcggtccagg ctccccagct ccggcaggtc cgccagcagt gtcatgctga cggccggtcc
      361 gacgccagga atggtctgca gcaggcctct gcgggcctgc ccggcctggg tttccagcag
      421 catgagctcg agctgggctt cgatgttggc gcgctcctga gacagccacg cgatcatctg
      481 gcgacagctg tcggcgacgg cctcgtccag ggtctgcttg aggcgcacct tttccatggc
      541 cgccatctcc accagctggc ggcggcgggt ggacaaggca cggatctcga aggccttggc
      601 gtcgggaacg gggcgggcgg cgtcctgcat caacagagcg aagcgcgcga tcagtcccgc
      661 gtccagcctg tcggtcttgg cccgtcgtcg tccttcggcc acccgcaggg cccggatccg
      721 cttggggtcg atcacgccga cctcgaagcc ggcctcggcc agactgcgca caagacgcgc
      781 gccgtaaccg cctatcgact ccagcaccac cttgcccgcg ccctcgcgct tcaaccgcgc
      841 caccagggct acaatccccg acggggcgtt ggcctcccgg aacaccttgc ccgacggggc
      901 cagacccacg tcgagccaat ctcggccgac atccacgccg gccgcgcatg catgcgtcat
      961 agctccgctc ctgtgttcga gctgaagcgc caagccctcc gcccgtgcga ctgttcggaa
     1021 aatctgactg gcgagcgcgc ccttgccggc atctcggcgt caaaaccagg gacccaacga
     1081 gctacgcccg ccaaccatca agccaaggct cgacgcctat cgcaatacac agggacccag
     1141 gggcgaccgc actgcgccgg ccactgggtc ccggacagcc tctgcgaggc ttccgggatg
     1201 acaaggtttg gagcaggttg agcgctcctg ttgatgaggc ccaccatggc tctgcgcaaa
     1261 catctcgctc tcgccgctct tggggccgtc cttggcgcga tcctgctgac cacagcgccc
     1321 ggcgtcgcgg ccgaggaaca gccctggcgg ctggtctggg ccgaagagtt cgacggcgac
     1381 cggctggacg agacccgctg gacccacgcc gccgactgcg gcgggggcgg caataacgag
     1441 cggcagtgct acgtcgtcag cccggacacc gtgtcggttc gcgacggcct gctgcggctg
     1501 acggcgatca agcgcaagac gcggggactg gccaatccgt gggccggccc caccgggccg
     1561 atgaagaccg gcgactacgc ctcgggcaag atcgtcagcc agggcaaggc cagctggctg
     1621 tatggccgga tcgaggcgcg ggcccgggtt ccgggcgggc agggcgtttg gccggccatc
     1681 tggatgatgc ccgagaccaa cgcctatggc ggctggccgc gctcgggcga gatcgacatc
     1741 ctggagacca tcaatctggg cgcgccgtgc gacacctgcg agggcgggcg cgagaaccgc
     1801 accttcggca cgatacattt tggcaagggc cccggcgccg atcaccgcca gctgggcggc
     1861 cacaccgtca gcgcgcccac cgccgacgac ttccacgtct atgcggtgga atggtcggcg
     1921 ggcaagatcg actggttcgt cgacggcgtc cgctatttca ccgctacggc ggaagactgg
     1981 aagccagccg acggcgaggc ctcgcccgcc ccgttcgacc agcccttcca cctgatcctc
     2041 aacctcgcct tcggcggggc ctggcccgag ggcgccaacg ccaggggcgt ggacgaggcg
     2101 gccctgccgg cgacgatgga ggtggactgg gtgcgggtga gtcagcggta ggggtctttc
     2161 tggatcactc gccccctcct gaccgctatc gcggtcgtcc tcccccatag ggggaagatg
     2221 agtttcatcc gcccccgctg ggggcggacg acctgcgaag caggtcaggt gagggcaagt
     2281 ctggcggaca cgggctcgta gcctcacgcg gcatcagcca ctgaaggggt cggggaggcg
     2341 cgaagcggcc gggg
//
LOCUS       DAOMEO010000482         2351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4838_length_2351_cov_1.963850, whole genome shotgun sequence.
ACCESSION   DAOMEO010000482 DAOMEO010000000
VERSION     DAOMEO010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_2351_cov_1.963850"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..350)
                     /locus_tag="PLH31_11015"
     CDS             complement(9..350)
                     /locus_tag="PLH31_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HQR89763.1"
                     /translation="MTPGQALLLRIYTDENAMTGDRRFFDVLVERARSMGLAGATVLR
                     GLRGYGASSTLHGGKAFDLAPNLPVVVEIVDIEASLRAFIATLQPEDEIGLVTLEKVE
                     VVRYGGHRHHY"
     gene            complement(352..>1053)
                     /locus_tag="PLH31_11020"
     CDS             complement(352..>1053)
                     /locus_tag="PLH31_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89764.1"
                     /translation="RENGKRRVVVQANVRGNDLGSFVADAQAKVDAQVKLPAGSWIEW
                     SGQFENLKAAQSRLALVVPLCFLLIFALLYMALGGFAPAVAVFSAIPLALAGGVFALA
                     LRGIPFSVSAAVGFIALSGVAVLNGLVMMTAIRQRLAAGFELEKAIIDGAMERLRPVM
                     MTGLVASLGFVPMALATETGAEVQRPLATVVIGGLITATALTLFVLPAICRFVLRKTA
                     KDLREHPAETQEQGA"
     assembly_gap    1054..1063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1064..>2351)
                     /locus_tag="PLH31_11025"
     CDS             complement(<1064..>2351)
                     /locus_tag="PLH31_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR89765.1"
                     /translation="VSGNLMSLGALDFGLIVDGAVIIVENSLRRLAERQHREGRLLTL
                     SERLQETREAAREMISPTVYGQAIILLVYAPLLTFTGVEGKTFSPMAITVMLALAAAF
                     VLSLTFIPAMIALVIRGKVAEKEVAVVRWTKVRYEPLLRKAVARPWPVIGAAVGMFLV
                     AMVTFSFLGREFTPQLDEKDLAVQALRIPSTSLEQSLRMQRQIERTIATFPEVQFVYS
                     KTGTAEVASDPMPPNASDSFIILKPQDEWPNKKETKAELVTRMEKKLEGLTGNVYEFS
                     QPIQMRFNELIAGVRGDVAIKVYGDDLDAMTASAGQIAKALQSVKGAADVKVEQTSGF
                     PTLDVSLDRDAIGRLGLTVEEVADTLAVAMGGRESGLVFQGDRRFDIVVRLPDATRND
                     LDAVGALPVMLPERAGGGARLSVPLRDVARFSYSEGL"
BASE COUNT          418 a          783 c          778 g          362 t
ORIGIN      
        1 cccatcgcct agtagtgatg gcggtggccg ccgtagcgga ccacttcgac cttctccagc
       61 gttaccaacc cgatttcgtc ctcgggctgc agggtggcga taaaggctcg gagcgaggcc
      121 tcgatatcga cgatctcgac gacgaccggc aggttgggcg ccaggtcgaa cgccttgccg
      181 ccgtgcaggg tgctggaagc gccgtagcct cgcagcccgc gcaggacggt ggcgccggcc
      241 agacccatcg accgggcgcg ttcgaccagg acgtcgaaga agcgccgatc gccggtcatg
      301 gcgttctcat cggtatagat gcgtagcagc agtgcttggc ccggggtcat cttaggctcc
      361 ttgctcctga gtttccgccg gatgctcgcg caggtccttg gcggtcttgc gcagcacgaa
      421 ccggcagatg gcgggcagga cgaacagggt cagggccgtg gcggtgatca ggccgccgat
      481 gaccacggtg gccagcgggc gctggacctc cgccccggtc tcggtggcca gcgccatggg
      541 cacaaagccc aacgaggcca ccagacccgt catcatcacc ggccggagcc gctccatggc
      601 cccgtcgatg atcgccttct ccaactcaaa cccagctgcc aagcgctggc ggatggcagt
      661 catcatcacc aggccgttga gcacggccac gccggacagg gcgatgaacc cgacggcggc
      721 cgagaccgag aacgggatgc cgcgtagcgc cagggcgaat accccgccgg ccagggccag
      781 gggaatggcc gagaacacgg cgacagcggg cgcgaagccc cccagcgcca tgtagagcaa
      841 ggcgaagatc agcaggaagc agagcgggac gaccagggcg agccgcgatt gcgccgcctt
      901 gaggttctcg aactgcccgc tccactctat ccacgacccg gccggcagct tgacctgggc
      961 gtcgaccttg gcctgggcgt cggcgacaaa cgagccgaga tcattgcccc gaacattggc
     1021 ctggacgacg acgcggcgct tgccgttctc acgnnnnnnn nnnaggccct ccgaatagga
     1081 gaagcgggcg acgtcgcgca gcggcaccga aaggcgcgcg ccgccccctg ccctttcggg
     1141 cagcatcacg ggcagcgctc cgaccgcgtc cagatcgttg cgagtggcgt caggaagccg
     1201 cacgacgatg tcgaagcgcc ggtcgccctg gaagaccagg ccggactcgc gcccgcccat
     1261 ggccaccgcc agggtgtcgg cgacctcctc gaccgtcagg cccaggcgac cgatggcgtc
     1321 gcgatcgagc gagacgtcca gggtcgggaa ccccgaggtc tgctcgacct tgacgtcggc
     1381 cgcgcccttg acgctttgca acgccttggc gatctggcca gcgctggcgg tcatggcgtc
     1441 caagtcatcg ccatagacct tgatcgccac atcgccgcgc acaccggcga tcagttcgtt
     1501 gaaccgcatc tggatgggct ggctgaactc gtagacgttg cccgtcaggc cttcgagctt
     1561 cttctccatg cgggtgacga gctcagcttt cgtctccttc ttgttgggcc attcgtcctg
     1621 cggcttgaga atgatgaagc tgtcggacgc attgggaggc atcggatcgc tggcgacctc
     1681 ggcggtgccg gttttggagt agacgaactg cacctccggg aaggtggcga tggtccgctc
     1741 gatctgccgc tgcatgcgaa gcgactgctc cagcgaggtc gaagggatcc gcagcgcttg
     1801 gacggccagg tccttctcgt cgagctgcgg cgtgaactcc cgccccagga acgagaaggt
     1861 gaccatggcc accaggaaca tgccgaccgc cgcgccgatc acgggccagg gccgggctac
     1921 ggccttgcgc agtagcggct cgtagcgaac cttggtccag cggaccacgg ccacttcctt
     1981 ttcggcgacc ttgccgcgga tcaccaaggc gatcattgcc gggatgaagg tcagcgacag
     2041 cacgaaggct gcagccaggg ccagcatcac ggtgatggcc atcggcgaga aggtcttgcc
     2101 ctcgacgccg gtgaaggtca gcagcggtgc gtagaccagc aggatgatcg cctgaccgta
     2161 gacggtcggc gagatcatct cgcgcgccgc ctcgcgggtt tcctgaagcc gctccgacaa
     2221 ggtcagcaaa cgaccctcgc ggtgctggcg ttcggccagg cggcgcaggc tgttttcgac
     2281 gatgatcacc gcgccatcga cgataagacc aaagtccagc gcgcccaggc tcatcagatt
     2341 gcccgacacc c
//
LOCUS       DAOMEO010000483         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4870_length_2341_cov_2.543307, whole genome shotgun sequence.
ACCESSION   DAOMEO010000483 DAOMEO010000000
VERSION     DAOMEO010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4870_length_2341_cov_2.543307"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLH31_11030"
     CDS             <1..1427
                     /locus_tag="PLH31_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR89766.1"
                     /translation="ARHPRLRWVSLSLAGLVQTIPSLALLALFFPLLLLLSGLSERML
                     GHGFSALGFLPSLLALTLYSMLPILRNTAAGLTGVDPAVVEAARGVGMTDRQRLWRVE
                     LPLALPVIMAGVRTAAVWTIGAATLSTPVGQTSLGDYIFSGLQTEDWVAVLVGCAASA
                     VLAMAVDQLLGLIESGVGQRNRRRWVAGLAGLAIGLVGALSPLASVALSRGQPGYVIG
                     AKNFSEQYVLAELMAGQLEARGARVERRINLGSAVAYRAVAASEIDAYVDYSGTLWAN
                     VLGRKDNPGRQAVLDQLRTGLKDRDGVVLLAPLGFENAYALAMRRDRAESLGIRTLAD
                     LAAKGGGLTMGGDLEFFSRPEWTGVEQAYGLKFRARRQFQPTFMYRALASGEADVISA
                     FSSDGRIAADDLVLLGDPKGALPPYDAVILVSPKRAGDRRLIAALSPLAGTIDVKTMQ
                     AANYAIDRDAGKVSAAEAAKMLTR"
     gene            complement(1542..>2341)
                     /gene="hutU"
                     /locus_tag="PLH31_11035"
     CDS             complement(1542..>2341)
                     /gene="hutU"
                     /locus_tag="PLH31_11035"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQR89767.1"
                     /translation="PESVNRAARASMAVHVQAMLDFQAAGVPTVDYGNNIRQMALEEG
                     VANAFDFPGFVPAYIRPLFCRGIGPFRWAALSGDPEDIARTDAKVKALIPDNPHLHNW
                     LDMAAEKIKFQGLPARICWVGLGDRHRLGLAFNAMVASGELKAPVVIGRDHLDSGSVA
                     SPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGFSQHAGMVIVAD
                     GTQAAARRLERVLWNDPASGVMRHADAGYEVAAECAREKGLDLPGIL"
BASE COUNT          310 a          818 c          831 g          382 t
ORIGIN      
        1 cggcccgcca tccgcgtctg cgctgggtgt cgctgtccct ggcggggctg gtccagacca
       61 ttcccagcct ggccctgctg gccttgttct tcccgctgct gctgctgctg tcgggcctct
      121 ccgagcggat gctgggccac ggcttttcgg ccctgggttt cctgccctcg ctgctggccc
      181 tgacgctcta ttcgatgctg ccgatcctgc ggaacacggc ggccggcctg accggcgtcg
      241 atccggccgt ggtcgaggcg gcgcgcggcg ttggcatgac cgaccgccag cgcctgtggc
      301 gggtggagct gcccctggcc ctgccggtca tcatggccgg ggtgcggaca gcggcggtgt
      361 ggaccatcgg cgcggccacc ctctcgaccc ccgtcggcca gacctcgctg ggcgactaca
      421 tcttctcggg cctgcagacc gaggactggg tggcggtgct ggtcggttgc gcggcctcgg
      481 cagttctcgc gatggcggtc gaccaactgc tgggcctgat cgagagcggt gttggccagc
      541 gcaaccgcag gcgctgggtc gccggcctgg cgggcctggc gatcggtctg gtcggcgcgc
      601 tctcgcccct tgccagcgtg gccctgtcga gaggccagcc cggctatgtg atcggggcca
      661 agaacttctc cgagcaatat gtcctggccg agctgatggc cggccagctg gaggcccggg
      721 gcgcccgggt cgagcggcgg atcaatctgg gctcggccgt cgcctatcgc gccgtcgcgg
      781 ccagcgagat cgacgcctat gtcgactatt ccggcacgct ctgggccaat gtgctgggcc
      841 gcaaggacaa ccccggccgt caggcggtgc tggaccagct gcggaccggt ctgaaggacc
      901 gcgacggcgt cgtgctgctg gccccgctgg gtttcgagaa cgcctacgcc ctggccatgc
      961 gccgcgaccg cgccgagagt ctgggcatca ggaccctggc ggatctcgcc gccaagggcg
     1021 ggggtctgac catgggcggc gacctggagt tcttctcgcg gcccgaatgg accggcgtcg
     1081 agcaggccta tggcctgaag ttccgggcca ggcgccaatt ccagcccacc ttcatgtacc
     1141 gcgccctggc ctccggcgaa gccgacgtga tctcggcctt ctccagcgac gggcgaatcg
     1201 cggccgacga tctggtcttg ttgggcgacc ccaagggagc cctgccgccc tatgacgcgg
     1261 tgatcctggt ctcgccgaag cgggccgggg accggcggct gatcgcggcc ctgtcgccgc
     1321 tggcggggac catcgacgtc aagaccatgc aggcggccaa ctacgcgatt gaccgggacg
     1381 cggggaaggt cagcgcggcg gaggctgcga agatgctgac gcgttagatg ctccccctct
     1441 gggggagctg tcgcggagcg actgaggggg ctttcttggc ataggccgcg ctggcccctt
     1501 ccggccctct gggccacctc ccccagaggg ggaggatctg gtcagagtat ccccggcaga
     1561 tccagcccct tctcccgcgc acactccgcc gcgacctcgt accccgcgtc ggcatggcgc
     1621 atgaccccac tggccgggtc gttccacagc acgcgctcca gccgccgggc cgccgcctgc
     1681 gtgccgtcgg cgacgatgac catgccggcg tgctgcgaga agcccatgcc caccccgccg
     1741 ccatggtgca gcgacaccca ggtggcgccc gacgccgtat tcagcagggc gttcagcaga
     1801 ggccagtccg acacggcgtc ggacccgtcg cgcatcgcct cggtctcgcg attgggcgag
     1861 gcgacggaac cggagtccag gtgatcgcgg ccgatgacga cgggcgcttt gagctcgccg
     1921 ctggcgacca tcgcgttgaa ggccaggccc agccggtggc ggtcgcccag gccgacccag
     1981 cagatccgcg ccggcagacc ctggaacttg atcttctccg ccgccatgtc gagccagtta
     2041 tgcagatgcg gattgtcggg gatcagcgcc ttcaccttgg cgtcggtcct ggcgatgtct
     2101 tccggatcgc ccgacagcgc cgcccagcgg aacggcccga tgccccggca gaacagcggt
     2161 cggatatagg ccggtacgaa gccggggaag tcgaaggcgt tggcgacgcc ttcctccagc
     2221 gccatctggc ggatgttgtt gccgtaatcg accgtgggca cgccggcggc ctggaagtcg
     2281 agcatggcct ggacgtggac cgccatcgag gcgcgggcgg cgcggttgac gctttccgga
     2341 t
//
LOCUS       DAOMEO010000484         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4880_length_2337_cov_2.573620, whole genome shotgun sequence.
ACCESSION   DAOMEO010000484 DAOMEO010000000
VERSION     DAOMEO010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_2337_cov_2.573620"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLH31_11040"
     CDS             complement(<1..242)
                     /locus_tag="PLH31_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR89768.1"
                     /translation="MRALLIVSSLFVLAGCATSGAKTSSLHPATTIDAARFYTGTWYE
                     IGRRPMSLTDGCVAGGTTYTPQGGVKVKVLDFCRDGS"
     gene            complement(345..644)
                     /locus_tag="PLH31_11045"
     CDS             complement(345..644)
                     /locus_tag="PLH31_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89769.1"
                     /translation="MIASLVLAAALSNAAPSQTASDLLIPASQPREAVAILNCLVKDD
                     GHLTACTVEGESAPELGVGDAALKMAAQFQVDLLGPDGKSRAGSYVQVPVRIRIR"
     gene            complement(750..2123)
                     /locus_tag="PLH31_11050"
     CDS             complement(750..2123)
                     /locus_tag="PLH31_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89770.1"
                     /translation="MFEFGRDLKRLFGVDAGAESKAAHREGLTGGDTSLLELLDIRLL
                     TNEARSADVAAGRIGTRDRGARLLEAAIVWHELARRSGDAAALRKAASHAEAAGKIFE
                     SERRLEALASTRCEQAVLAILGADLFGDEGLNAAAGAALTLARTAPGQRCGLAMALVA
                     GLDGRAGLAENNIDRALAAVQGYDAPLKALAAAKRVKGGSARLLLADQRAARIELILT
                     CGARLKDRALTQLAITEATAAAAALDPDFEPLTWSRLEGLRGSALVQLGELDGELTLI
                     ADGVEALTAALEPLAMDHSPMDWARAQAALGVGLQAIGEATTSDRAFEQAVTCYDRAT
                     QVLKTQPALALRAVVANNRALSLARCAELTADLAVLDAAEMAFKTELSAQPGVRDPAS
                     WAVAQMNLARLYEARVEITGRDDGRLDRAAVALACAFDVFSELGLRSLTDLAAQGLHR
                     LKAAHAH"
BASE COUNT          384 a          791 c          804 g          358 t
ORIGIN      
        1 gagccgtcgc ggcagaagtc cagcacctta accttgacgc ccccctgggg cgtataggtg
       61 gtgccgcccg caacgcagcc gtcggtgagc gacatgggtc gccggccaat ctcataccat
      121 gtgccggtgt agaaccgcgc cgcgtcgatc gttgttgcgg gatgcaggga actggttttg
      181 gcgccactcg tcgcgcagcc cgcgaggacg aacaggctcg aaacaatcag caaggcgcgc
      241 atggggatct ccgaccaacg gagacagcta cggctcaacg cgcctggggg atgcaggcgc
      301 ggcgcctagg gcgcgatagc cgaaagtgta cgcgctaaaa gactctagcg gatccggatc
      361 cgcaccggca cctgcacata ggaccccgcg cggctcttgc cgtcggggcc cagcaggtcg
      421 acctggaact gggcggccat cttcagggcc gcgtcgccga cgcccagctc aggggcgctt
      481 tcgccctcga cagtgcaggc cgtcaggtgg ccatcgtcct tcaccaggca gttgaggatc
      541 gcaacagctt cgcgcggctg tgacgccggg atcagcaggt ccgaggcggt ctgggacggg
      601 gcggcgttgg aaagagcggc ggccagaaca agcgaagcaa tcacgatttc gtccctgtta
      661 ttttcttgat cggctgcatc gctttggcaa tgcgccgacc tttgtttcga cggggcgacc
      721 ctgccgcccc cttgttgtcc cgatccgggt cagtgggcgt gagccgcctt caaccgatgc
      781 agcccctgtg ccgccaggtc ggtcagcgac cgcaggccca gctcggaaaa cacgtcgaac
      841 gcgcaggcca gggccacggc ggcccggtcc agccggccat cgtcgcggcc cgtgatctcg
      901 acccgggctt cgtacaggcg cgccagattc atctgggcca cggcccacga ggccgggtcg
      961 cgtacgcccg gctgggcgga aagttcggtc ttgaaggcca tctcggccgc gtccagcacc
     1021 gccagatcgg cggtcagctc ggcgcagcgc gccaggctga gggcgcggtt gttggcgacc
     1081 accgcgcgca acgccagggc cggctgggtc ttgagcacct gcgtagcccg atcatagcag
     1141 gtgacggcct gctcgaaggc ccgatccgag gtcgtggcct cgccaatggc ctgcaggccg
     1201 acgcccagcg cggcctgggc gcgcgcccag tccatcgggc tgtgatccat ggcgagcggt
     1261 tcaagagcgg cggtcagggc ctcgacgcca tcggcaatca gggtcagttc gccgtccagc
     1321 tcgcccagct gcaccagggc cgagccgcga agaccttcca gtcgcgacca ggtcaggggc
     1381 tcgaagtcgg gatccagggc ggctgcggcg gctgtggcct cggtaatcgc cagctgggtc
     1441 agggcgcggt ccttcaggcg cgcgccgcag gtcaggatca gttcgatgcg cgcggcgcgc
     1501 tggtcggcca gcagcagccg ggccgacccg cccttgacgc gcttggcggc ggccagagcc
     1561 ttcagcggcg cgtcataacc ctgcacggcg gccagggcgc ggtcgatatt gttctcggcc
     1621 aggccggcgc gaccgtcgag gccggcgacc agggccatgg ccaggccgca ccgctgtccc
     1681 ggcgcggtgc gcgccagggt cagggcggcg ccggcggcgg cattcaggcc ctcgtcgcca
     1741 aacaggtccg cgcccaggat agccagaacg gcctgctcgc agcgggtcga ggccagagcc
     1801 tccagtcggc gttcgctttc aaagatcttg cccgcggcct cggcgtgcga ggcggccttg
     1861 cgcagggcgg cggcgtcgcc agaccggcgg gccagttcgt gccagacgat ggcggcttcc
     1921 agcaagcgcg cgccgcgatc ccgggtcccg atccggcccg cagccacatc ggccgagcgg
     1981 gcctcgttgg tcagcagcct tatgtcgagc agttcgagca gcgaggtgtc gccgcccgtc
     2041 aggccttcgc gatgggcggc cttagactct gccccggcgt cgacgccgaa aagacgtttc
     2101 agatcacgac cgaactcgaa catcgacgcg catctccgca cacggaccgt cccacaccgg
     2161 gacgtccggc aggggttcaa cgcatcagga gcaaagccgg agccaacgca cttaacaagt
     2221 ggttaatagg tgttgatggc gcattaacca taaatatttc ccggacctta agaaagtgag
     2281 cgtcgcgcgg ccagatccgg gaacttttgg ctcagtttgg cacacttttt gccttcc
//
LOCUS       DAOMEO010000485         2337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4881_length_2337_cov_2.498685, whole genome shotgun sequence.
ACCESSION   DAOMEO010000485 DAOMEO010000000
VERSION     DAOMEO010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4881_length_2337_cov_2.498685"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLH31_11055"
     CDS             <1..766
                     /locus_tag="PLH31_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HQR89771.1"
                     /translation="FEADPQSVFISGLSAGGAMTAVMLATYPELFAGGAIIAGLPYGV
                     ARSVSDALRAMGRGAGRPAPALAGLVLDAAPRPAAPLRLVIWHGDADATVHPANGRDL
                     SLQWAAALNLKALPDAAPPGPGRTLSVWRGEGPAPSVLEMHMVEGLAHGTPLSTRRDC
                     DVGTVAPFMIEMGLCSTLETAAFWGLSESAAFRATPVRNKGEPSLTQPTAAFEAQTRS
                     KPVGIGDQVMGAVSGHVSPQVQDIIAKALRAAGLLS"
     gene            complement(864..1637)
                     /locus_tag="PLH31_11060"
     CDS             complement(864..1637)
                     /locus_tag="PLH31_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQR89772.1"
                     /translation="MNRLLIASIDRFGPLKDDARTSLGACSQTVVTVSPGHSLVQRGE
                     PSRLRVLAKGLAVRQHVLADGARQILGFVTPGDLLDLGGLFAGGDHEVRALNACEVRQ
                     TTAREARSLLRQHPSLLAALCRAVMTEAKLQRNWMVGLGRRSALARTANLFCEIYARQ
                     KAVGLAAEGRCRLPVLQADIADALGLSVVHTHRVLQTLKKSGLASLRDGDLSIEDWDR
                     LVGLAEFDPDCFRPQHDPAELAATRGRHTLPALTAAQQA"
     gene            complement(1777..>2337)
                     /locus_tag="PLH31_11065"
     CDS             complement(1777..>2337)
                     /locus_tag="PLH31_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HQR89773.1"
                     /translation="EITPAAAPIPAAEMDPSPALSLLAKAPPGLVGRKVGCLVTDGVD
                     DALVAELKAAVEAAGAKLAIIAPTISGVTTAAGALLAADFRIDGGPSVLFDAVALLPS
                     DEGGAALALEATAVNFVRDAFGHLKVIAYVPAAAPLFVKGGLSDANPDADAGLINLPK
                     ATVADFITAASGGRIWDREPLVRKVF"
BASE COUNT          368 a          799 c          810 g          360 t
ORIGIN      
        1 cttcgaggcc gatccccaaa gcgttttcat cagcgggttg tcagcggggg gcgcgatgac
       61 ggcggtcatg ctcgcgacct atccggagct cttcgcgggc ggcgcgatca tcgccggcct
      121 gccctacggt gtggccagga gcgtgtcgga cgcccttcgc gccatgggcc ggggcgccgg
      181 gcgacccgct cctgcgctgg cgggtctggt gctggacgcc gcgccgcgcc cggccgcgcc
      241 gctgaggctt gtcatctggc acggggacgc cgacgcgact gtgcatcccg ccaacggccg
      301 ggatctctcc ctgcaatggg cggcggccct gaacctgaag gccctgccgg acgccgcgcc
      361 ccccggaccc ggccgaaccc taagcgtctg gcgtggcgag gggcctgccc cttcggtgct
      421 ggaaatgcac atggtcgagg ggcttgctca tggaaccccg ctctcgaccc gccgggattg
      481 cgacgtcggc acggtcgccc ccttcatgat cgagatgggg ctttgctcga cgctggagac
      541 tgccgccttc tggggccttt cagagtcggc cgctttccgc gcgacgccag ttcggaacaa
      601 gggggagcca tccctgaccc agccgacggc cgcgttcgag gctcaaacaa ggtcaaagcc
      661 tgtgggcata ggcgaccagg tgatgggcgc tgtgtcggga catgtgtctc ctcaggtcca
      721 ggatatcatc gccaaggcgc ttcgcgctgc aggcctcctg agctaggttc ggcctgacgc
      781 tgacgccgta ctcgaccatc aaacagagga cgggcgggcg atggcgaggc cgcctgggca
      841 gatctacttg cgcgatgggc ggctcaggcc tgttgggcag ccgtcagggc gggcagcgta
      901 tgacgccccc tagtggcggc cagttcggcc ggatcgtgct ggggccggaa acaatccggg
      961 tcgaactcgg ccagacccac aagccgatcc cagtcctcga tgctcaggtc gccatcgcgc
     1021 aaggacgcca gcccggactt cttcagggtc tgcagcacac ggtgggtatg gaccaccgag
     1081 aggcccaggg cgtcggcgat atcggcctga agcacgggaa gcctgcaacg gccttctgcc
     1141 gcgaggccga cggccttctg ccgggcatag atttcacaga acaggttggc tgttcgcgcc
     1201 agcgccgagc gacgacctag acccaccatc cagtttcgct gtaattttgc ttcggtcatc
     1261 actgcccggc acagggcggc cagcaggctg ggatgctggc gaagcaggga tcgagcctca
     1321 cgggcggtgg tctggcgcac ctcgcaggca ttgagcgcgc gcacctcgtg atcgccgccc
     1381 gcgaacagtc cgccaaggtc gagcagatcg ccgggggtga cgaaacccag aatctgccgc
     1441 gcgccatccg ccagcacgtg ttgccggacc gccaggccct tggccaggac ccgaagtcgg
     1501 gagggctcgc ccctctggac cagactgtgg cccggggaga ccgtgaccac ggtctgggag
     1561 catgccccca ggctggtccg ggcatcatcc ttgagcggac caaatcggtc gatgctggcg
     1621 atcagcaggc ggttcatgac atagccttcg gcggcgcggc ggaccgtggg aatgcgatct
     1681 cgcccacgcc tgagctcaac cctctggcaa ccctcatgtt ccctctggcc gtctcttgcc
     1741 ggggcccggc tgacgcgggg ccccggcgcc cggagactag aacaccttgc ggaccagggg
     1801 ttctcgatcc cagatccggc cgcccgacgc cgcggtgatg aagtcggcga ccgtcgcctt
     1861 gggaaggttg atcaagccgg cgtcagcgtc gggattggcg tccgacagtc cgcccttcac
     1921 aaacaggggc gcagcggcgg gcacataggc gatgaccttc aggtggccga aggcgtcgcg
     1981 gacgaagttc accgccgtcg cctccagggc cagggcggcg ccgccctcat ctgacggcag
     2041 cagcgccacg gcgtcgaaca gcaccgaagg gccgccatcg atccgaaaat ccgccgccag
     2101 cagggcgcct gccgcggtgg tcacgccgct gatggtcggg gcgatgatcg ccagctttgc
     2161 gccggcggcc tcgacggccg ccttcagctc ggccaccagg gcgtcgtcga caccatcggt
     2221 gaccaggcag ccgaccttgc ggccgaccag gcccggcgga gccttggcca ggaggctcaa
     2281 ggccggtgac gggtccatct cggcagccgg gatcggagcc gcagctggcg tgatttc
//
LOCUS       DAOMEO010000486         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4884_length_2336_cov_2.971942, whole genome shotgun sequence.
ACCESSION   DAOMEO010000486 DAOMEO010000000
VERSION     DAOMEO010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_2336_cov_2.971942"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="ybgF"
                     /locus_tag="PLH31_11070"
     CDS             <1..307
                     /gene="ybgF"
                     /locus_tag="PLH31_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQR89774.1"
                     /translation="AYVANYPDTPRTPEARYWLGETLFVREAYGDAAGAYLGAVRGWP
                     QSSWAPDATLKLSRSLVALKKPADACKTLDELARRYPKAPPAVVSRAASTRVAAKCG"
     gene            298..>1207
                     /gene="tilS"
                     /locus_tag="PLH31_11075"
     CDS             298..>1207
                     /gene="tilS"
                     /locus_tag="PLH31_11075"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQR89775.1"
                     /translation="MRLGEVSAALDRRLDPHSAAPVAVGFSGGGDSLALLLLTLDWAR
                     AHGRPVLSLTVDHQLQPDSAAWTREAVAAAQARGARALALVWTGEKPTTGLPAAARRA
                     RHALLAEAARAAGARVLLLGHTADDIAEAAVMRAEGSTVSDPRDWSPSPVWPEGRGIF
                     LLRPLLDQRRAELRAWLRARGETWLDDPANDDPRSARARARRSAPTLAMPAPPIAPPP
                     VFEGDPLGVIALPRDAPAAHLAAACLCVAGTERPPRGERLQRLMARLRSGEVFTATLA
                     GARIEAAADAVRVFRDAGETARGGLAD"
     assembly_gap    1208..1217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1218..1530
                     /locus_tag="PLH31_11080"
     CDS             <1218..1530
                     /locus_tag="PLH31_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HQR89776.1"
                     /translation="ALAPGQTGVWDGRYEIFAGGAPVTVRALKGLASSLSKADQAALR
                     TAPVAARPALPALVLASGAVTCPALGGPALAGADGVRIRPLFLDRFRAATGLIDQECV
                     T"
     gene            1607..>2336
                     /locus_tag="PLH31_11085"
     CDS             1607..>2336
                     /locus_tag="PLH31_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQR89777.1"
                     /translation="MNFRNAAIWLVIVAVLVGMFVVNQTSRTQAGGEISYSQLLRKVD
                     AGEIKSADIAGQIVQAKDGSGNVLTVNAPNNPDELVNRLVARGADVKFKGGGVSLLAI
                     LVNLLPIILVIGVWVFFMRQMQGGAKGAMGFGKSKARLLTENKNKVMFDDVAGVDEAK
                     EELTEIVDFLKDPAKFQRLGGKIPKGALLIGPPGTGKTLIARAVAGEAGVPFFTISGS
                     DFVEMFVGVGASRVRDMFEQAKKNA"
BASE COUNT          325 a          832 c          816 g          353 t
ORIGIN      
        1 ggcctatgtg gccaactatc ccgacacccc gcgcacgccg gaagctcgct actggctggg
       61 cgagaccctg tttgtacggg aagcctatgg cgacgccgcc ggcgcctatc tgggcgcagt
      121 gcgggggtgg ccgcagagca gctgggcgcc cgacgccacg ctgaaactgt cacgctcgct
      181 ggtggcgctg aagaagcctg cggacgcctg caagacgctg gacgagttgg ccaggcgcta
      241 tcccaaggcc ccgccggccg tcgtgtcgcg cgccgcctcg acccgcgtcg cggccaagtg
      301 cggctaggcg aggtttcggc cgccctcgac cggcggctgg atccccacag cgccgcgccc
      361 gtcgcggtgg gcttctccgg cggcggcgac tcgctggccc tgctgctcct gacgctggac
      421 tgggccaggg cccatggccg gcccgtgctg agcctgaccg tcgaccacca gctgcagcct
      481 gacagcgccg cctggacccg cgaagccgtc gccgcggcgc aagcccgcgg ggcgagagcc
      541 ctggcgctcg tctggaccgg cgaaaagccg accaccggcc ttcccgccgc cgcccgccgc
      601 gcgcgccacg ccctgctggc cgaggccgcc cgcgccgccg gggctcgggt cctgctgcta
      661 ggccatacgg ctgacgacat tgccgaggcc gccgtcatgc gcgccgaggg ctccaccgtc
      721 tccgatccgc gcgactggtc gccgtcgccg gtctggccgg aggggcgagg catctttctg
      781 ctgcgccccc tgctggacca gcgtagggct gagctgcgcg cttggctgcg agcgcggggc
      841 gagacttggc tggacgatcc ggccaatgac gatccccgat cggcccgcgc cagggcgcgg
      901 cgatcggcgc cgaccctcgc catgcccgcc ccgccgattg cgccgccgcc ggtcttcgag
      961 ggcgacccgc tgggcgtcat cgccctgccg cgagacgccc ccgccgccca tctcgccgcc
     1021 gcctgcctgt gcgtggccgg aaccgagcgg cccccacgcg gcgagcgcct ccagcgtctg
     1081 atggcccgcc tgcggtcggg cgaggtcttc accgccaccc tggccggggc gcggatcgag
     1141 gccgccgccg acgccgtgcg ggtctttcgg gacgccggcg agacggcgcg cggcggcctg
     1201 gctgatcnnn nnnnnnnggc cctcgcgccg ggccagaccg gcgtctggga cggccgctat
     1261 gaaattttcg ccggcggcgc gccggtcacc gtgcgggcgc tcaagggcct ggcctccagc
     1321 ctgtcgaaag ccgaccaggc cgccctccgc accgcccctg tcgccgcccg cccggccttg
     1381 cctgccttgg tgttggcaag cggcgcggta acctgtcccg ccctcggcgg cccggctctt
     1441 gcaggcgcag acggcgtgcg aatccggcct ctcttcctgg atcgtttcag ggccgcgacg
     1501 ggcctgatcg accaagaatg cgtgacctga caacgcgcgc gtggcgcaag cgctctcgac
     1561 cccctatctt gggaagacga aggctatcgt tggaaagacg acgcgtatga atttcaggaa
     1621 tgcggccatc tggctggtga tcgttgcggt gctggtcggc atgtttgtcg tcaaccagac
     1681 ctcgcgcacc caggccggcg gcgaaatcag ctactcgcag cttctgcgca aggtggacgc
     1741 gggcgagatc aagtcggccg acatcgccgg gcagatcgtc caggccaagg acgggtctgg
     1801 caatgtgctg accgtcaatg ctccgaacaa tccggacgaa ctggtcaatc gcctggtggc
     1861 gcggggcgcc gacgtcaagt tcaagggcgg cggcgtcagc ctgctggcca tcctggtcaa
     1921 tctgctgccg atcatcctgg tgatcggggt gtgggtgttc ttcatgcggc agatgcaggg
     1981 cggggccaag ggcgccatgg gctttggcaa gtccaaggcc cgcctgctga ccgaaaacaa
     2041 gaacaaggtg atgttcgacg acgtcgccgg cgtcgatgag gccaaggaag aactgacgga
     2101 gatcgtcgac ttcctgaaag acccggccaa gttccagcgc ctgggcggca agattcccaa
     2161 gggcgccctg ctgatcggtc ctcccggtac gggtaagacc ctgatcgccc gcgcggttgc
     2221 gggtgaggcg ggcgtgccct tcttcacgat ctcgggttcg gactttgtcg agatgtttgt
     2281 cggcgtcggc gccagccgcg tgcgcgacat gttcgagcag gccaagaaga acgcgc
//
LOCUS       DAOMEO010000487         2332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4888_length_2332_cov_2.634168, whole genome shotgun sequence.
ACCESSION   DAOMEO010000487 DAOMEO010000000
VERSION     DAOMEO010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_2332_cov_2.634168"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLH31_11090"
     CDS             <1..441
                     /locus_tag="PLH31_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose transferase"
                     /protein_id="HQR89778.1"
                     /translation="VRGWEQGAQAIIARARDEQAAGGLTSIAMDDRFLFNVTAYYGRD
                     YFRTPGAPPLKMWVHEARPQNQAETEAPLTATFGRRALVASLEGDYRPEIEQDFARTS
                     GLEIVRVRLDRKHVRRTHLFIAEDFRPLPRDPVTGLPPKPKGKP"
     gene            complement(438..797)
                     /locus_tag="PLH31_11095"
     CDS             complement(438..797)
                     /locus_tag="PLH31_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HQR89779.1"
                     /translation="MLLAAQIATGLVLLIHVYIVLLETVLFDSRGKKVFGLTDEAAAI
                     LKPAMSNQGCYNGFLVLALALGLFYPDPVTASAFKTFGLVCVAIAGVWGAATVKKSIL
                     FIQTVPAVIALVLGYLA"
     gene            complement(810..>2332)
                     /locus_tag="PLH31_11100"
     CDS             complement(810..>2332)
                     /locus_tag="PLH31_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HQR89780.1"
                     /translation="IEDVRDALRKHVEAHDETLNHIADAVAIFSQTRRLAFHNTAFAE
                     LWGLEPAWLADRPTHGEILDRLRQRRRLPETIDYAKWKAEELAHYEDLGPQADDMWGL
                     PDGRTLKLVRQPHPLGGMLLLYSDITGELKLKAQYNALIQVQQATLDKLNDAVAVFGS
                     DGRLRLHNEAFETFWNVTAFALEAAVDFEGVVDLCVPRLHDLSFWRELKGRVADPDPQ
                     MRAPTSGEVRTSDGRIVLYQSRPLPDGATLIGFTDVTDTRDLQSALADRSAALAEAER
                     LKRDFVGNVSYELRTPLTTIIGYSELLERAEGLHERGRNHVAAVRAAATQLARSIDDV
                     LDMAQIDAGEMALEIQDIRVVDLLVDARERAMKDATLGGVTLTIECDEDVGLIRGDLK
                     RLSQTLDHLVENALRQTPPDGIVTLSARRALGEVRLDVKDTGRGVPFHVQAHIFDRFV
                     GRDRGGPGLGLALVKALVELHGGWVALESEPGAGSTFTCHLPETQQPGAMQPELSF"
BASE COUNT          383 a          799 c          804 g          346 t
ORIGIN      
        1 gtgcgcggct gggagcaggg cgcccaggcg atcatcgccc gggcgcgcga cgaacaggcg
       61 gccggcggcc tgaccagcat cgccatggac gaccgcttcc tgttcaacgt caccgcctat
      121 tacggccgag actacttcag gacgcccggc gcgccgcccc tgaagatgtg ggtccatgag
      181 gcccgtccgc agaaccaggc cgagaccgag gcgccgctga ccgccacctt cggtcgccgc
      241 gccctggtcg ccagcctgga gggcgactat cgccccgaga tcgagcagga cttcgcgcgg
      301 acctcaggcc tggaaatcgt ccgcgtgcgc ctcgaccgca agcacgtgcg tcgcacccac
      361 ctgttcatcg ccgaagactt ccgccccctg ccccgcgatc cggtgacggg tttgccgccc
      421 aagccgaagg gcaaacctta ggctaggtag cccagcacca aggcgatcac ggccggaacg
      481 gtctggatga aaaggatcga cttcttgacg gtcgccgcgc cccagacgcc ggcgatggcg
      541 acgcagacca ggccaaaggt cttgaacgcc gaggccgtca ccgggtcggg atagaagagg
      601 cccagcgcca gggccaggac cagaaagccg ttatagcagc cctggttcga catggccggc
      661 ttgaggatgg cggcggcctc gtcggtcagg ccgaagacct tcttgccccg gctgtcgaac
      721 agcaccgtct ccagcagcac gatatagacg tggatcagca ggaccaggcc ggtggcgatc
      781 tgggcggcga gaagcatggc gcgacctcgt tagaacgaca gttccggctg catggcgccc
      841 ggctgctggg tctcgggcag gtggcaggtg aaggtcgagc cggcgcccgg ctcgctctcc
      901 agcgccaccc agccgccgtg cagctcgacc agcgccttga ccaaagccag gcccaggccc
      961 ggcccgccgc gatcccgacc gacgaagcgg tcgaagatgt gagcctggac gtggaacggc
     1021 acgccgcgcc cggtgtcctt gacgtccagc cgcacctcgc ccagcgcgcg gcgcgccgac
     1081 agggtgacga tgccgtcggg cggggtctgg cgcagggcgt tctcgaccag atggtccagg
     1141 gtctgggaca ggcgcttgag gtcgccgcgg atcaggccga catcctcgtc gcactcgatg
     1201 gtcagggtca cgccgcccag ggtcgcgtcc ttcatcgccc gctcgcgagc gtcgaccagc
     1261 aggtcgacga cgcggatatc ctggatttcc agcgccatct cgccggcgtc gatctgggcc
     1321 atgtcgagca cgtcgtcgat cgaacgggcc agctgggtgg cggcggcgcg cacggcggcc
     1381 acatggttgc ggccgcgctc atgcaggccc tcggcccgct ccagtagctc cgaatagccg
     1441 atgatcgtgg tcagcggcgt gcgcagctca taggacacgt tgcccacaaa gtcgcgcttc
     1501 agccgctcgg cctcggccaa agctgcggaa cgatcagcca gggcgctttg caggtcgcgg
     1561 gtgtcggtga cgtcggtgaa gccgatcagg gtcgcgccat cgggcagcgg ccggctctga
     1621 tagagcacga tccggccgtc ggaggtgcgc acctcgcctg aggtcggcgc gcgcatctgc
     1681 ggatcaggat cggcgacccg ccccttcagc tcgcgccaga acgacaggtc gtgcaggcgc
     1741 ggcacgcaca gatcaaccac gccttcgaag tccacggcgg cttccagcgc aaaggccgta
     1801 acattccaga aggtctcgaa cgcctcgttg tgcagccgca gccggccgtc ggagccgaac
     1861 accgcgacgg cgtcgttcag cttgtccagc gtcgcctgct gcacctggat cagggcgttg
     1921 tactgggcct tcagcttcag ctcgccggtg atgtcggaat agagcagcag catgccgccc
     1981 agcgggtgag gctgacgcac cagcttcagg gtacggccat cgggcaggcc ccacatgtcg
     2041 tcggcctggg gccccaggtc ctcgtagtgc gccagctcct cggccttcca cttggcgtag
     2101 tcgatggtct cgggcaggcg gcggcgctgg cgcaggcgat cgaggatctc gccatgggtc
     2161 ggacggtcgg ccagccacgc gggctcaagc ccccacagct cggcgaaggc ggtgttgtgg
     2221 aaggccaggc gccgggtctg gctgaagatc gccacggcgt cggcgatgtg gttcagggtc
     2281 tcgtcgtggg cctcgacatg cttgcgcagg gcgtcgcgga catcctcgat ct
//
LOCUS       DAOMEO010000488         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4895_length_2327_cov_2.417694, whole genome shotgun sequence.
ACCESSION   DAOMEO010000488 DAOMEO010000000
VERSION     DAOMEO010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_2327_cov_2.417694"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLH31_11105"
     CDS             <1..550
                     /locus_tag="PLH31_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQR89781.1"
                     /translation="IDHATGPWGVKIARIEIKDLTPPADITNAMARQMKAERERRAVI
                     TEAEGEKQAAIARAEGQKQSAILQAEGRREAAFRDSEAREREAEAEAKATAFVSEAIS
                     KGDVNAINYFIAQKYVEAFGDLARSPNAKTVIVPADFAGLTGTVAGVAELVKTLGGGG
                     GTVAPTPPVPRTNTPTAGVKRT"
     gene            555..1010
                     /locus_tag="PLH31_11110"
     CDS             555..1010
                     /locus_tag="PLH31_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQR89782.1"
                     /translation="MDALTSFYAMHPFWVWLALAAIFLAIEVGTGSGWLLWPAASSAV
                     VGLLALALPMNLPVEIALFAILTIATTYLARRFLRPVLEGDAPDLNDPLLRLIGRDGE
                     VLSTFEAGVGRVFVDGKEWQARLEGGPPLPGQRIQVVGVDGATLTVRAY"
     gene            1110..2036
                     /locus_tag="PLH31_11115"
     CDS             1110..2036
                     /locus_tag="PLH31_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR89783.1"
                     /translation="MTGIISVTGLTKTYASGHQALKSVDLDIRQGEIFALLGPNGAGK
                     TTLISIICGIVNPSTGTILADGHDVVRDYRAARSKIGLVPQELNTDAFETVWATVRFS
                     RGLFGKPRNDAYVEKVLRDLSLWDKKDAKIMTLSGGMKRRVMIAKALSHEPTILFLDE
                     PTAGVDVELRRDMWEMVRALRASGVTIILTTHYIEEAEEMADRIGVISKGEIILVEDK
                     DVLMRKLGKKQLTLQLQTPLTAVPVALSDYALELAADGHELIYTFDAQAEETGIAELL
                     RRLGEQGVDFKDLHTSQSSLEDIFVSLVRDAA"
     gene            2033..>2327
                     /locus_tag="PLH31_11120"
     CDS             2033..>2327
                     /locus_tag="PLH31_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR89784.1"
                     /translation="MNLYAIKSIYRFEMARWFRTLAQSILAPVISTSLYFVVFGSAIG
                     SRMQAIDGVSYGAFIIPGLVMLSLLSESISNAAFGIYMPKWAGTIYELLSAPVS"
BASE COUNT          402 a          774 c          753 g          398 t
ORIGIN      
        1 catcgaccac gctaccggtc cgtggggcgt caagatcgcc cggatcgaga tcaaggacct
       61 gacgccgccg gccgacatca ccaacgccat ggcccggcag atgaaggccg agcgcgagcg
      121 ccgcgccgtg atcaccgagg ccgagggcga gaagcaggcc gccatcgccc gcgccgaagg
      181 ccagaagcaa tcggccatcc ttcaggccga ggggcgtcgg gaagcggcct tccgcgactc
      241 tgaagcccgc gagcgggaag ccgaagccga ggccaaggcg accgccttcg tgtcggaagc
      301 catctccaag ggcgacgtca acgccatcaa ctacttcatc gcccagaaat atgtcgaagc
      361 gttcggcgat ctggcccgct cgcccaacgc caagaccgtg atcgtgcccg ccgacttcgc
      421 cggtctgacc ggaacggtcg cgggcgtggc cgaactggtc aagacgctgg gcggtggcgg
      481 cgggacggtc gcgccgacgc cgcccgttcc acgcaccaac accccgaccg ccggcgtgaa
      541 gcggacctga cccgatggac gcgctgacga gcttctatgc gatgcatccc ttttgggtct
      601 ggctggccct ggcggcgatc ttcctcgcca tcgaagtcgg gaccggcagt ggctggctgc
      661 tatggccggc cgcgtcctcg gcggtggtcg gtcttctggc cctggccttg ccgatgaacc
      721 tgcccgtcga gatcgccctg ttcgccatcc tgaccatcgc caccacctat ctggcccgcc
      781 ggtttctgag gccggtgctg gagggcgacg cacctgatct caatgacccg ctgctgcgcc
      841 tgatcggccg cgacggcgag gtgctgtcga ccttcgaggc cggcgtgggt cgggtctttg
      901 tcgacggcaa ggaatggcag gcccgtcttg aaggcggacc gcccttgccg ggccagcgca
      961 tccaggtcgt gggcgttgat ggcgcgacgc taacggtgcg cgcctactaa cttggccgtt
     1021 tgaggctcga cggctggcgc ctggctagac gcgtccccat ctggccgttc acaccgccgc
     1081 ttgcatcgcg cggatcttca gggggacccg tgacgggcat catttcggta acgggtctga
     1141 ccaagaccta tgcttcaggc catcaggccc tgaagtcagt ggatctggac attcgacagg
     1201 gcgagatctt tgcgctgctg ggccccaacg gggccggcaa gaccacgctg atcagcatca
     1261 tctgcggcat cgtcaatccc agcacgggga cgatcctggc cgatggccat gatgtggtcc
     1321 gcgactatcg cgccgcccga tccaagatcg gcctggtgcc gcaggagttg aacaccgacg
     1381 ccttcgagac ggtctgggcc acggtgcgct tctcgcgggg tctgttcggc aagcctcgca
     1441 acgacgccta tgttgagaag gtgctgcgcg acctgtccct ctgggacaag aaggacgcca
     1501 agatcatgac gctgtcgggc ggcatgaagc gccgggtgat gatcgccaag gcgctgagcc
     1561 acgagccgac catcctgttt ctggacgagc ccaccgccgg cgtcgatgtc gagctgcgcc
     1621 gcgacatgtg ggagatggtc cgcgccctgc gcgccagcgg cgtcaccatc atcctgacca
     1681 cccactatat cgaggaagcc gaggagatgg ctgaccggat cggggtgatc agcaagggcg
     1741 agatcattct ggtcgaggac aaagatgtgc tgatgcgcaa gctgggcaag aagcagctga
     1801 ccctgcagct gcagaccccg ctgaccgccg tgccagtcgc cctgtctgac tacgcgctgg
     1861 aactggccgc cgacggccac gagctgatct acaccttcga cgcccaggcc gaggagaccg
     1921 gcatcgccga acttctgcgc cgccttggcg agcagggcgt cgacttcaag gacctgcaca
     1981 ccagccagag ctcgctggaa gacatcttcg tcagcctggt gagggacgcg gcatgaacct
     2041 ctacgcgatc aagtccatct atcgtttcga gatggcgcgg tggttccgta ccctggccca
     2101 gagcatcctg gcgccggtga tctccacctc gctgtatttc gtggtgttcg gctctgcgat
     2161 cggttcgcga atgcaggcca ttgacggagt cagctacggc gccttcatca tcccgggcct
     2221 cgtgatgctg tccctgctca gcgagagcat ctccaacgcc gccttcggca tctacatgcc
     2281 caaatgggcg ggcacgatct atgagctgct gtcggcgccg gtctcat
//
LOCUS       DAOMEO010000489         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4899_length_2325_cov_3.214978, whole genome shotgun sequence.
ACCESSION   DAOMEO010000489 DAOMEO010000000
VERSION     DAOMEO010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4899_length_2325_cov_3.214978"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2286)
                     /locus_tag="PLH31_11125"
     CDS             complement(112..2286)
                     /locus_tag="PLH31_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HQR89785.1"
                     /translation="MDDAILAPPPTTDQERTGEPRPPFPLYALKDHDTFLVADAFGDI
                     GGVGDGLFHNDTRVLSKYRLTLGGHAPSHLSSSLSQDNVFFNCHATNQNLPLLGAAAA
                     PHGTIHLERKRFLWDQRCYERLTFANYSRDLVMLPVSLEFGADFNDMFEVRGSKRDAR
                     GLRTTPVAEGRSVRFAYTGLDAVERSSVISFSEPPGRLSSSRADFLFMLSADDRFELY
                     VEIGPTLSPPPTRQRWREAAARARLSMRQRTRRGARLHSSGRLFNEWLEKSRADLALL
                     TTELDTGPYPYAGIPWFSTPFGRDAIITAWQVLWIQPELARGVLAYLGKHQAVEISAF
                     QDSAPGKIMHETRRGEMTALGELPFGRYYGGVDTTPLFVGLACAYAERTGDMAFIDGL
                     WPTLLNAVGWIEQFGDSDGDGLIDYARGAASGLSNQNWKDSEDSVFHADGRFPAGPIA
                     VVEVQGYAYAAFKGMADLARRRGDGANGRRWAERAETLRQTVEARFWMEDQGFYAMAI
                     DGAGQPCRVRGSNPGHLLFSGLPSHERAKRVTETLLGSAFHAGWGLRTLASGEPRFNP
                     MSYHNGSIWPHDTALCVLGMARYGERQGVVRMLAGLFETAAHFEMRLPELFCGFSRAA
                     GEPPVAYPVACLPQAWAAGSVFMMLQACLGVTVDGWKGKVTLSDPRLPIGVDRLDIDS
                     IVLGDKVVDIRFSRSGDKTRARINRQGSTAGVWPGRLEPFSI"
BASE COUNT          406 a          741 c          792 g          386 t
ORIGIN      
        1 tcgcttgggg agggccagcg cgttttccag tcattgtgga acggttttcg gatcgaaaac
       61 ggtctagact actgacttca cgcccgtttt tgcctgactg tggtgaagcg ttcagatcga
      121 aaagggctcc agtcggcctg gccagacccc agccgttgaa ccctggcgat tgatcctggc
      181 ccgggtcttg tcgccagaac ggctgaaacg aatatccacc accttgtcgc ccagaacgat
      241 gctgtcgatg tccagacggt ccacgccgat gggcaggcgc gggtcgctca gagtgacctt
      301 gcccttccag ccatccaccg tgacgcccag gcaggcctgc agcatcatga agaccgagcc
      361 ggccgcccag gcctggggca ggcaggcgac ggggtaggcc accgggggtt cgccggcggc
      421 gcgtgagaag ccgcagaaca gctcgggcag gcgcatctcg aaatgggcgg cggtttcgaa
      481 caggccagcc agcatccgca ccacgccctg gcgctcgcca tagcgggcca tgcccagaac
      541 gcagagggcc gtatcgtggg gccagatcga cccgttatga tacgacatcg gattgaagcg
      601 tggctcaccc gaagccaggg ttcgaaggcc ccagccggcg tggaaggccg atcccagcag
      661 ggtctcggta acccgcttgg cgcgctcgtg gctgggaagg cctgaaaaca gcaggtggcc
      721 gggatttgag cctctcaccc ggcagggctg gccggcgccg tcgatggcca tggcgtagaa
      781 gccctggtct tccatccaga agcgcgcctc gactgtctgg cgcagggttt cagctcgctc
      841 agcccagcgc ctgccgttgg cgccgtcccc gcgacgccgg gcaagatcgg ccatgccctt
      901 gaaggcggca taggcgtagc cctgcacctc gacgacggcg atgggcccgg cagggaacct
      961 gccatcggca tggaagacgc tgtcttcgct gtccttccag ttctggttgg acaggccgct
     1021 ggccgccccg cgcgcatagt cgatcagccc gtcgccatcg ctatcgccga actgttcgat
     1081 ccagccgacc gcgttcagca gggtgggcca caggccgtcg atgaaggcca tgtcccccgt
     1141 ccgctcggca taggcgcagg ccaggccgac gaacaggggg gtggtgtcga cgccgccgta
     1201 gtaccggccg aacggaagct cgcccagggc ggtcatctcg ccacggcggg tctcatgcat
     1261 gatcttgcca ggcgcgctgt cctggaaggc cgagatttcg accgcctggt gcttgcccag
     1321 ataggccaga acgccccgcg ctagttcggg ctggatccac agcacctgcc aggcggtaat
     1381 gatggcgtcg cgaccgaacg gggtcgaaaa ccaggggatg ccggcatagg ggtaggggcc
     1441 agtgtccagt tcggtggtca gcagggctag gtcggcgcgg gacttttcca gccactcgtt
     1501 gaacagccgg cccgaactgt gtagccgcgc gccgcgccgg gtgcgctggc gcatgctgag
     1561 ccgggctctc gccgccgctt cgcgccagcg ttgccgggtt ggcggcggcg acagggttgg
     1621 cccgatctcg acatacagtt caaaccggtc atcagccgac agcatgaaca ggaagtcggc
     1681 ccgcgaggac gacaggcggc ccgggggttc ggaaaagctg atcacgctac tgcgctcgac
     1741 tgcgtccagg cccgtatagg caaagcgcac cgagcgccct tccgccacag gtgtggtgcg
     1801 cagtccgcgc gcatcgcgct tggacccgcg cacctcgaac atgtcgttga agtccgcgcc
     1861 aaactcgagg ctgacgggca gcatgaccag gtcgcggctg taattggcga aggtcaggcg
     1921 ctcgtagcag cgctggtccc acaggaaacg cttgcgctcc aggtggattg ttccatgcgg
     1981 cgccgcggcc gcgccgagca gcggcaggtt ttgattggtg gcgtggcagt tgaagaagac
     2041 gttgtcctgc gacaacgacg aactgagatg cgacggcgcg tggccgccca gggtgagccg
     2101 atactttgac agcacccggg tgtcattgtg gaacagtccg tcgccgaccc cgccaatgtc
     2161 gccaaaggca tcggcgacca ggaaggtgtc gtggtccttc agcgcataga gcgggaaggg
     2221 cgggcgcggc tcgcccgtcc tttcctgatc cgtcgtcggg ggtggagcga gaatagcgtc
     2281 atccatcaga tcgacctgtc gtgtccggcg ctttgcgcca agttt
//
LOCUS       DAOMEO010000490         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4921_length_2313_cov_2.873339, whole genome shotgun sequence.
ACCESSION   DAOMEO010000490 DAOMEO010000000
VERSION     DAOMEO010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_2313_cov_2.873339"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLH31_11130"
     CDS             complement(<1..475)
                     /locus_tag="PLH31_11130"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQR89786.1"
                     /translation="MQREIMTVAEMTAADRAAVARGVSIGALMERAGIAVAEAVRARF
                     SHRRVVIWCGPGDNGGDGYVAARHLRRKGWSVRVETSAPPATDAARRAAARWKGETGP
                     LSPEPSPADIYIDALFGAGLSRPLEGDVARLARACSLLGKPVVAIDTPSGVSGDTG"
     gene            complement(486..677)
                     /locus_tag="PLH31_11135"
     CDS             complement(486..677)
                     /locus_tag="PLH31_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89787.1"
                     /translation="MAEHPNAAVFSHFVALNAAVGVATGEASVAAFRPDHCSITTFDI
                     RDGRLILKEKGREAQSQVL"
     gene            complement(635..1048)
                     /locus_tag="PLH31_11140"
     CDS             complement(635..1048)
                     /locus_tag="PLH31_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR89788.1"
                     /translation="MTRVHVIRHGCPASTWGGADEDPGLDEVGRQQAIKVMEVLMALP
                     EGERPRRVVSSPLRRCRETAEPMAQALGVALTIDPRVGEIPTPAGMPAAERPAWLRLA
                     VNGARSSVIRTMKPGPSASRRLWPNIRTLRCSVTL"
     gene            complement(1053..>2313)
                     /locus_tag="PLH31_11145"
     CDS             complement(1053..>2313)
                     /locus_tag="PLH31_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HQR89789.1"
                     /translation="VYTETGGNSPQQAVNVICDRIAQGQSELALVAGAEFLGSLMKRL
                     KGGLGFDGWGDDLTEAPGRIGDPRPGVTPQEAAHGLGYPVNTYPLFENALRARDQRSL
                     EDHQVRLGELFSPFSKVAAANPNAWFPVERSPAELVTVTDRNRMISYPYPKYLNAIME
                     VDQSAAVLICSVRKARELGVPEDRWVFLHGCADAADLWYPMDRQDFHSSPAIRLTGER
                     AFEMAGIGLADIDIIDLYSCFPSAVQVAAEEFGLALDDPRGLTVTGGLPYFGGPGNNY
                     ALHSIAEMMARLRARPGAYGLCTANGWFLTKQSVGIYSTKPVDGDWIREAPSVIQDRI
                     DALPHPEIIDRPTGPAVIETYTVVHAREGARMGIVIGRDAAGRRFVAHTPDDAATLLD
                     LEAREGVGRTGVVGPHPDGVRNLFVPD"
BASE COUNT          417 a          797 c          721 g          378 t
ORIGIN      
        1 ggcccgtatc gccggacacc ccgctgggcg tatcaatcgc gaccaccggc ttgcccaaca
       61 gactgcaggc ccgggccaat ctggcgacat cgccttccag aggtcgcgac aggcccgcgc
      121 caaacagcgc gtctatataa atgtccgcag ggcttggctc tggcgacaaa ggaccggttt
      181 cgcccttcca tcgggctgcg gcccgcctcg cagcatcggt ggccggcggg gcggaagttt
      241 ccacgcgcac cgaccagcct ttgcggcgga gatggcgtgc ggcgacatag ccatcgccgc
      301 cgttatcgcc ggggccgcac cagatcacga cacgccggtg cgagaacctc gccctcaccg
      361 cttcagccac tgcgatcccg gctcgctcca tcaaggcgcc gatcgaaacg ccgcgcgcga
      421 cagcggcgcg gtccgccgcg gtcatttcgg cgacagtcat gatctcgcgt tgcacgacga
      481 ctccctcaga gcacctggct ctgagcttcg cgtcctttct ccttgaggat caagcgccca
      541 tcgcgaatgt cgaacgtcgt gatcgagcaa tgatccggcc gaaacgccgc cacgctggcc
      601 tcaccggtcg cgaccccgac agcggcgttg agcgctacaa agtgactgaa caccgcagcg
      661 ttcggatgtt cggccacagc cgccgcgacg ccgagggccc aggcttcata gtcctgatca
      721 ccgacgatct ggctccattc accgccaaac gtagccaggc gggacgctcg gcggcgggca
      781 tccctgcggg cgtcggaatc tcgccgacgc gcggatcgat cgttagcgct acgcccagcg
      841 cctgcgccat gggctcagcc gtctcgcggc agcgccgcag aggcgacgag acgacgcgcc
      901 ttggccgctc tccctccggc aaggccatca agacctccat caccttgata gcctgttgtc
      961 gcccgacctc atcaaggccc ggatcctcgt cggctccccc ccaggtggag gccgggcaac
     1021 cgtggcgaat gacatgaacg cgggtcatga gctcagtccg ggacaaagag gtttcgcacg
     1081 ccatcgggat gcgggccaac cacgccggtt ctccccacgc cttcgcgtgc ttccagatcc
     1141 aagagcgtag ccgcatcatc aggcgtgtgg gccacgaaac gccgccccgc cgcatctcga
     1201 ccgataacga tgcccatgcg ggcgccctcg cgggcgtgga cgactgtata ggtctcgatc
     1261 accgcaggtc cggtcggtcg gtcgatgatc tctgggtgcg gcagggcgtc gatgcgatcc
     1321 tggatcacgg acggagcctc gcggatccag tctccgtcga cgggcttggt tgaatagatg
     1381 ccgacagact gcttggtcag gaaccagcca ttggccgtgc agagcccgta ggcgccaggc
     1441 cgggccctta accgggccat catctcggcg atcgaatgca gggcgtagtt gtttccgggt
     1501 ccgccaaaat acggcaggcc gccagtaacc gtcagaccgc gcggatcgtc cagcgccagg
     1561 ccgaactctt ccgccgccac ctgaacggcc gacgggaagc aggaatagag gtcgatgatg
     1621 tcgatatcgg ccagaccgat gccggccatc tcgaaggccc gttcgccagt caggcgaatg
     1681 gccgggctgg aatggaagtc ctgccggtcc atcgggtacc agaggtcagc ggcgtcggcg
     1741 caaccgtgca ggaagaccca ccggtcttcg ggcacgccca gttctcgcgc cttccgaacg
     1801 ctgcagatca gcaccgcagc agactggtct acctccatga tggcgttcag gtacttggga
     1861 tagggatagc tgatcatgcg gttgcggtcg gtgaccgtga ccagttcggc cggtgatcgc
     1921 tcgaccggaa accacgcatt cgggttggcg gcggcgacct tgctgaaagg cgagaaaagc
     1981 tccccgagcc gaacctgatg gtcctccagc gagcgctgat cacgcgcccg tagcgcattc
     2041 tcgaatagcg gataggtgtt taccggatag cccagcccgt gtgcggcctc ctgtggcgtc
     2101 acgcctggac gcgggtcgcc aatccggccc ggagcctccg tgaggtcatc gccccacccg
     2161 tcaaagccga ggccaccctt caggcgcttc atcaatgaac cgagaaactc ggcgccggcg
     2221 accagggcca actcagactg cccctgagcg atcctatcgc aaatgacgtt gaccgcctgt
     2281 tgcgggctat tgccgcctgt ctcggtgtag acg
//
LOCUS       DAOMEO010000491         2311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4923_length_2311_cov_2.614805, whole genome shotgun sequence.
ACCESSION   DAOMEO010000491 DAOMEO010000000
VERSION     DAOMEO010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4923_length_2311_cov_2.614805"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..581
                     /locus_tag="PLH31_11150"
     CDS             111..581
                     /locus_tag="PLH31_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89790.1"
                     /translation="MGRPLATLAALGLLAASLAGCAHEQVADSGYRWAFLRDPGEAPR
                     LAYGRPFSDDVILMLSCSPGADSVRISAVGLNGREIVLASNRTESRYPAQRAESALVE
                     NGLLEARGQMSSPALAAFQHTGDLTVLTNGERHNLHASREDEGQVKAFFRSCAA"
     gene            complement(582..>2311)
                     /gene="dxs"
                     /locus_tag="PLH31_11155"
     CDS             complement(582..>2311)
                     /gene="dxs"
                     /locus_tag="PLH31_11155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQR89791.1"
                     /translation="ETPKDILIWDVGHQAYPHKILTGRRDRIKTLRQGGGLSGFTKRA
                     ESEYDPFGAAHAATSISAALGFCAARDAKGETNKVVAVIGDGSMSAGMAYEAMNAATD
                     TTKQLTVILNDNDMSIAPPVGGMSAYLANLVSGGAYRKARKLGKTVVEKLPTPMREAA
                     RKAEEYARGMVTGGTFFEELGFHYVGPIDGHDMDALVSVLKNAREFEDKPVLVHVVTQ
                     KGKGYAPAEGAADKLHAVVKFDVVTGQQHKAAAGPPSYTKVFAQELVKQAERDSKIVA
                     ITAAMPSGTGLDIFQKAFPERTFDVGIAEQHAVTFAAGLAADGMKPFAAIYSTFLQRG
                     YDQVVHDVAIQRLPVRFAMDRAGLVGADGPTHAGTFDIGFMGALPGMILMAAADELEL
                     ARMVATAVEIDDRPSAFRYPRGEGLGLEMPVGCADPLEIGKGRIVREGTSVAIVSFGT
                     RLSESLKAADLLAARGLPATVCDARFAKPLDIDLLLRLAREHEAIITVEEGAMGGFGA
                     FVLQALAEHGALDRGLKIRTLVLPDIFQDQDKPEVMYAQAGLDAEGIMRGALSALGID
                     NVSAAGRRA"
BASE COUNT          373 a          790 c          765 g          383 t
ORIGIN      
        1 agcccatcac gaccttcagg cgcgacccga aagggctcgc gcctgaagca tttctgcggc
       61 ccacgaaacc gccaagctcc agcggcgttc tctcaccagg agaactactc atgggacgcc
      121 cgctcgccac acttgccgct ctgggactgc tggccgccag ccttgcgggc tgcgctcatg
      181 aacaggtcgc cgacagcggt tatcgctggg cgttcctccg tgatcccggc gaagcgccgc
      241 gcctggccta tggtcggccg ttcagcgacg acgtcatcct gatgctcagc tgctcgcccg
      301 gcgccgacag cgtccggatc tctgcggtcg gtctgaatgg gcgagagatc gttctggcgt
      361 ccaaccgcac cgaaagccgc tatcccgccc agcgggccga aagcgccctg gtcgaaaacg
      421 gcctgctgga agcgcgcggc cagatgagct cgcctgccct ggccgccttc cagcacaccg
      481 gcgatctgac ggtgctgacc aacggcgagc gacacaacct ccacgccagc cgtgaagacg
      541 aaggtcaggt gaaagcgttc ttccggtcgt gcgccgcctg atcaggcccg gcggcccgcc
      601 gccgagacat tgtcgatccc cagggccgac aacgcgccgc gcatgatgcc ctcggcgtcg
      661 agaccggcct gggcgtacat gacctctggc ttgtcctgat cctggaagat atccggcaag
      721 accagcgtgc ggatcttcag gccgcgatcc agcgcgccgt gctcggccag ggcctgcagc
      781 acgaaggccc cgaagccgcc catggcgcct tcttccaccg tgatgatcgc ttcatgctcg
      841 cgggccagcc gcagcagcag gtcgatatcc agcggcttgg cgaagcgggc atcgcagacc
      901 gtggcgggca agccgcgcgc ggccagcaga tcggcggcct tcaggctttc gctcagacgc
      961 gtgccgaacg agacgatggc gacgctggtc ccctcgcgga cgatgcggcc cttgccgatc
     1021 tccagcgggt cggcgcagcc caccggcatt tcgaggccga gaccttcgcc gcgcggatag
     1081 cggaaggcgc tggggcggtc gtcgatctcg acagcggtgg ccaccatccg cgccagttcc
     1141 agctcgtcgg cggcggccat caggatcatg ccgggcagag cgcccatgaa gccgatatcg
     1201 aaggtgccgg cgtgggtggg accatcggcg ccgaccaggc cggcgcggtc catcgcaaac
     1261 cgtacaggca aacgctggat ggccacatcg tggaccacct gatcatagcc gcgctgcagg
     1321 aaggtcgaat agatcgccgc gaacggcttc atgccgtcgg cggcgaggcc ggcggcgaag
     1381 gtcacagcgt gctgctcggc gatgccgacg tcgaaggtgc gctctgggaa cgccttctgg
     1441 aagatgtcga ggcccgtgcc cgaaggcatg gcggccgtga tggcgacaat cttcgaatcc
     1501 cgctcggcct gcttgaccag ttcctgagcg aaaaccttgg tatagctcgg cggaccggct
     1561 gcggccttgt gctgctggcc ggtgacaacg tcgaacttga cgacggcgtg cagcttgtcg
     1621 gccgcgccct cggccggggc gtagcccttg cccttctggg tgacgacgtg gaccagcacc
     1681 ggcttgtcct cgaactcgcg ggcgttcttc agaacgctga ccagggcgtc catgtcatgg
     1741 ccgtcgatcg ggccaacata atggaagccc agctcttcaa agaacgtgcc gccggtgacc
     1801 atgccgcggg cgtattcctc ggccttgcgg gccgcttcgc gcatcggggt cggcagcttc
     1861 tcgaccacgg tcttgcccag cttgcgcgcc ttgcgatagg cgccgcccga gacgaggttg
     1921 gccagatagg cgctcatccc gcctaccggc ggggcgatcg acatgtcgtt gtcgttgagg
     1981 atgaccgtca gctgcttggt ggtgtcggtg gccgcgttca tcgcctcata ggccatgccg
     2041 gcgctcatcg agccgtcgcc gatcacggcc acgaccttgt tggtctcgcc cttggcgtcc
     2101 cgcgcggcgc agaagcccag cgccgccgag atcgaggtgg ccgcgtgagc cgcgccgaac
     2161 ggatcgtatt cgctctcggc ccgcttggtg aagcccgaca aaccgccgcc ctgacgcagc
     2221 gtcttgatgc gatcgcgacg tccggtgaga atcttgtgcg gataggcctg gtggccgacg
     2281 tcccagatca ggatgtcctt gggcgtctcg a
//
LOCUS       DAOMEO010000492         2308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4930_length_2308_cov_2.672437, whole genome shotgun sequence.
ACCESSION   DAOMEO010000492 DAOMEO010000000
VERSION     DAOMEO010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4930_length_2308_cov_2.672437"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="phoB"
                     /locus_tag="PLH31_11160"
     CDS             complement(<1..619)
                     /gene="phoB"
                     /locus_tag="PLH31_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HQR89792.1"
                     /translation="MTPYVLVVEDEDALATLLHYNLDKEGYQIAVAGDGEEALTMASE
                     RAPDLVILDWMLPKVSGIEVCRRLRGRAETRNVPIIMLTARGEESDRIRGLDTGADDY
                     VVKPFSMVELTARVRAVLRRIRPGLADDRITVGDIVIDRVAHRVKRQGKEVHLGPTEF
                     RLLDYLMQHPGRVFSREQLLDAVWGSDVYVEARTVDVHIGRLRKAL"
     gene            complement(714..>1043)
                     /locus_tag="PLH31_11165"
     CDS             complement(714..>1043)
                     /locus_tag="PLH31_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HQR89793.1"
                     /translation="TRSIERMGKLVQGRLKDVLDAYSASDLERATGVWSRDEEVDEHY
                     NAIFRELLTYMMGDPRTINACAHMLFVAKNLERIGDHATNIAEIIHFEITGEEILSKR
                     PKLDTPR"
     assembly_gap    1044..1053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1054..1380)
                     /locus_tag="PLH31_11170"
     CDS             complement(<1054..1380)
                     /locus_tag="PLH31_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HQR89794.1"
                     /translation="MTEHTVKSYGEELNHLTAEVTRMGGLAEAQVADAVECIARRDAP
                     LAQQVVARDDRLDTLQGEIERKAFRLIALRQPMAVDLRHAVAALKISMSLERCGDMAK
                     NIGKRAL"
     gene            complement(1400..2236)
                     /gene="pstB"
                     /locus_tag="PLH31_11175"
     CDS             complement(1400..2236)
                     /gene="pstB"
                     /locus_tag="PLH31_11175"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQR89795.1"
                     /translation="MPPASPDDSVSAPVIRTAAPQAQPPIGEIKIRAKDVNVFYGDKQ
                     ALFDVNIDIAAKSVTAFIGPSGCGKSTFLRCINRMNDTIPTAKVSGSIEIDGNDVNLK
                     SVDPVVLRSRVGMVFQKPNPFPKTIFENVAYGPRIHGLATGKAELEAIVESSLKKAGL
                     WNEVADRLHQPGTGLSGGQQQRLVIARAIAVGPEVILMDEPCSALDPIATAKIEELID
                     ELRSQFCIVIVTHSMAQAARVSQRTAFFHLGKLVEAGTTEEMFTNPRDTRTQDYITGR
                     FG"
BASE COUNT          350 a          700 c          823 g          425 t
ORIGIN      
        1 tcagcgcctt ccgcaggcgg ccgatgtgca cgtccaccgt ccgcgcctcg acatagacgt
       61 ccgagcccca gaccgcgtcc agcagctgtt cgcggctgaa cacccggccg gggtgctgca
      121 tcagatagtc cagcaggcgg aactcggtgg ggcccaggtg gacttccttg ccctggcgct
      181 tcacgcggtg ggcgacgcgg tcgatgacga tgtcgccgac cgtgatgcgg tcgtcggcca
      241 ggcccggacg gatgcggcgc agcaccgcgc ggacgcgggc ggtcagttcg accatcgaga
      301 acggcttgac cacatagtcg tcggccccgg tgtccaggcc ccggatgcgg tcgctctctt
      361 caccgcgggc ggtcagcatg atgatcggca cgttgcgggt ctcggcccgg ccgcgcaggc
      421 ggcggcagac ctcgatgccc gagaccttgg gcagcatcca gtccaggatc accaggtccg
      481 gcgcgcgttc gctggccatg gtgagggctt cctcgccgtc gccggccacc gcgatctggt
      541 agccttcctt gtcgaggttg tagtgcagga gggtggccag ggcgtcctcg tcctcaacca
      601 caagcacgta gggcgtcatg acttgatcct caagagcttg taaacaagat cgttttcaaa
      661 catttagagc gtgacaggcg ccgaaaccgg catccacttt cggctgtcac gctctaccgc
      721 ggggtgtcga gcttggggcg cttggagaga atctcctcgc ccgtgatctc gaagtgaatg
      781 atctcggcga tattggtggc gtggtcgccg atgcgttcca ggttcttggc tacgaacagc
      841 atgtgggcgc aggcgttgat cgtgcgcgga tcgcccatca tgtaggtcag cagttcgcgg
      901 aagatggcgt tgtagtgctc gtcgacctct tcatcgcggc tccacacgcc ggtggcgcgc
      961 tcgaggtccg aggcgctgta ggcgtccagc acgtccttca ggcggccctg gaccagcttg
     1021 cccatgcgct cgatcgagcg ggtnnnnnnn nnncagggcg cgcttgccga tgttcttggc
     1081 catgtcgccg cagcgttcca ggctcatcga gatcttcagg gcggcgacgg cgtggcgcag
     1141 gtccacggcc atcggctggc gaagggcgat caggcggaag gccttgcgct cgatctcgcc
     1201 ctgcagggtg tccagccgat catcgcgggc cacgacctgc tgggccaggg gcgcgtcgcg
     1261 gcgggcgatg cactcgacgg cgtcggcgac ctgggcctcg gccaggccgc ccatgcgggt
     1321 gacctcggcc gtcaggtggt tcagttcttc gccgtaggat ttgacggtat gttcggtcat
     1381 ggcgtgggaa tcctaccggt tagccgaagc ggccggtgat gtagtcctgg gtgcgggtgt
     1441 cgcgaggatt ggtgaacatc tcctcggtgg tcccggcctc gaccagcttg cccaggtgga
     1501 agaaggcggt gcgctgcgag acgcgggcgg cctgggccat cgagtgggtg acgatgacga
     1561 tgcagaactg gctgcgcagc tcgtcgatca gttcctcgat cttggcggtg gcgatcgggt
     1621 cgagcgccga gcagggctcg tccatcagga tcacttcagg accgaccgca atcgcgcggg
     1681 cgatgaccag acgctgctgc tggccgcccg acaggccggt gccgggctgg tgcaggcggt
     1741 cggcgacctc gttccacagg ccggccttct tgaggctgct ctcgacgatg gcttccagct
     1801 cggccttgcc ggtggccagg ccatggatgc gcgggccgta ggcgacgttc tcgaagatgg
     1861 tcttggggaa cgggttgggc ttctggaaca ccatgccgac gcgcgagcgc agcaccaccg
     1921 ggtcgacgct cttgagattg acgtcattgc cgtcgatctc gatcgagccc gagaccttgg
     1981 cggtcgggat cgtgtcgttc atccggttga tgcagcgcag gaaggtcgac ttgccgcagc
     2041 ccgacgggcc gatgaaggcg gtgaccgact tggccgcgat gtcgatattg acgtcaaaca
     2101 gggcctgctt gtcgccgtag aagacgttga cgtccttggc gcggatcttg atctcgccga
     2161 tcggcggctg ggcttgcggc gcggccgtgc ggatcacggg agcggagacg gaatcgtcgg
     2221 ggctggcggg gggcatggcg tgtccctgga cgagggaaaa ggcggaaggg ttcaggatca
     2281 tgtgactacc agcgacgctc gaagcgcc
//
LOCUS       DAOMEO010000493         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4934_length_2306_cov_2.782319, whole genome shotgun sequence.
ACCESSION   DAOMEO010000493 DAOMEO010000000
VERSION     DAOMEO010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4934_length_2306_cov_2.782319"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLH31_11180"
     CDS             <1..782
                     /locus_tag="PLH31_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQR89796.1"
                     /translation="LRQGRVIVHTFGDGDWKAVLDHLRDNRLIDVHNAPTSVSGGGPR
                     AAVVAKPPDPQRLDAARRLWEGGRAVQGTLCERHCRLRHIRRALPGPEILRHGGDTPV
                     SAYVQTRHRRPALMAAICDAEGAFTAVEVTYLAPNGQKAWDLRLARKTVGPVPAGTAA
                     RLDPAAPEMLVAEGVFTTLSATERFDLPGWALMSTRNLKTWCAPPGVRSVLIAADRGL
                     DGAASAQILAARLQRQGVAARIEFPPEPFGDWNDAEAALRD"
     gene            complement(840..1232)
                     /locus_tag="PLH31_11185"
     CDS             complement(840..1232)
                     /locus_tag="PLH31_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR89797.1"
                     /translation="MLLLIEDEVLIIDVLADALTDSGYEVVKAQNGTEALAEIEADAS
                     RFRAIITDIKLGSGPNGWEIAQRAREIVAQMPIVYMSGDSSHEWSSKGVPGSVVLAKP
                     FATGQLVTAVSTLATEADMHSPPSGNGA"
     gene            1527..>2306
                     /locus_tag="PLH31_11190"
     CDS             1527..>2306
                     /locus_tag="PLH31_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HQR89798.1"
                     /translation="MTLSVTATPVSNLVFVSLQDLALAPENIRFKEPADDGIPRLADT
                     IAAANVVIPLSIRPGAKGEKPFMVLDGRRRLFALQALLTAGRIEANHPVKCELFEDRA
                     AQAAAAMLSNAERAPIHVADVIVAIGKLRKSKMDTAAIAAALGYDDLEIKRLEALAHV
                     HGNVLKAFRKGSLTLKQVRLFARMPDKKQQGELAETALDGHFHDYQLHQVINGSRLTI
                     EDDRFGLVGMARYTAAGGRVESDLFAELADVLLDPGKLQDLW"
BASE COUNT          376 a          794 c          758 g          378 t
ORIGIN      
        1 tgctgcggca aggccgggtg atcgtccata ccttcggcga cggcgactgg aaggcggtgc
       61 tggatcatct gcgcgacaac cggctgatcg acgtccacaa cgccccgacc tcggtgtctg
      121 gcggcggccc ccgcgcggcc gtggtcgcaa agccaccgga cccgcagcgc ctggacgccg
      181 ccaggcggct ctgggagggc ggccgggcgg tccaggggac gctctgcgag cgccactgcc
      241 gcctgcgcca tatccggcgc gccctgcccg gccccgagat cctgcgccat ggtggagaca
      301 cgccggtctc cgcctacgtc cagacgcgcc atcggcggcc ggctctgatg gcggcgatct
      361 gtgacgcgga gggcgcgttc accgccgtcg aggtcaccta cctcgcaccg aacggccaga
      421 aggcctggga tctgcgcctg gcgcgcaaga cggtcggacc cgtgccggcc ggcacggccg
      481 cgcgactgga tccagcggcg ccggagatgc tggtggccga gggggtgttc accacgctct
      541 cggcgaccga acgcttcgac ctgcccggct gggccctgat gtcgacccgc aacctgaaga
      601 cctggtgcgc accgccgggc gtgcgttcgg tgctgatcgc cgccgatcgc ggcctggatg
      661 gcgccgcctc ggcgcagatc ctggcggctc gcctgcaacg ccaaggcgtc gcggcgcgga
      721 tcgagtttcc gcctgaaccc ttcggcgact ggaacgatgc tgaagctgcc ctccgagact
      781 gagccgacgg gtgatgaagc gcggccgggc gcgagttttc cgccagacat tgaccctccc
      841 tacgcgccat ttcctgatgg cggcgagtgc atgtcagctt cggtcgcgag cgtggaaaca
      901 gctgtgacga gttgccccgt cgcgaaaggc ttggccaaga caacgctgcc gggcacccct
      961 ttcgacgacc attcgtgaga gctgtcgccg ctcatgtaga cgattggcat ctgggcgacg
     1021 atctcccgcg cccgttgggc gatctcccag ccgttcgggc cagacccgag tttgatgtcc
     1081 gtaatgatgg cgcggaatct agaagcgtcc gcctcgatct cggcgagagc ttccgttccg
     1141 ttttgcgcct tgaccacctc atagccactg tctgttagcg cgtccgccaa gacgtcgatg
     1201 atgaggactt cgtcctcgat caataacagc accacacttg acgtcatcgg cgccccccgg
     1261 cgagcagaga cgcctaagcg ccggctgtac gatacaaggc atttcgccat ggatggttcc
     1321 aatgctctga attgttagtc agacgacggc gttcagcggg cctatggaca aggaccaacg
     1381 agcgtctgcg gcatcgcgca cgggtaaact tcgcccactt cgagggccgg tcagtcggcg
     1441 gctctggaaa ggagggaggt agggaggggg ttgggtgcgc tcaggggcag gatgaccctc
     1501 gcccacggcg ctggaaccct ggtcccatga ccctttctgt caccgctacc cccgtctcga
     1561 acctggtctt cgtctctctg caggacctgg cgctcgcccc ggagaacatc cgcttcaagg
     1621 agcccgccga cgacggcatc ccgcgcctgg ccgacaccat cgcggccgcc aacgtggtca
     1681 tcccgctcag catccgaccc ggcgccaagg gcgagaagcc gttcatggtg ctggacggcc
     1741 gccgccgtct gttcgctttg caggccctgc tcacggcggg ccgcatcgag gcgaaccatc
     1801 cggtgaagtg cgaactgttc gaggacaggg ccgcccaggc cgccgccgcg atgctctcaa
     1861 acgccgagcg cgcgccgatc cacgtcgccg acgtgatcgt ggccatcggc aagcttcgca
     1921 agtcgaagat ggataccgcc gccatcgccg cggcgctggg ctatgacgac ctggagatca
     1981 aacgccttga ggcgctcgcc cacgtccacg gcaatgtgct gaaggccttt cgcaagggca
     2041 gcctgaccct caagcaggtt cgcctgttcg cgcggatgcc cgacaagaag cagcagggcg
     2101 aactggcgga gaccgcgctc gacggtcact tccacgacta ccagctgcac caggtgatca
     2161 acggctcgcg cctgaccatc gaggacgacc gttttggcct tgtcgggatg gcccgctaca
     2221 ccgccgccgg cgggcgggtc gagtccgacc tgttcgccga gctggccgat gtcctcctag
     2281 atcctggcaa gctgcaggat ctgtgg
//
LOCUS       DAOMEO010000494         2306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4935_length_2306_cov_2.286095, whole genome shotgun sequence.
ACCESSION   DAOMEO010000494 DAOMEO010000000
VERSION     DAOMEO010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4935_length_2306_cov_2.286095"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..257
                     /locus_tag="PLH31_11195"
                     /pseudo
     CDS             2..257
                     /locus_tag="PLH31_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517967.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <268..894
                     /locus_tag="PLH31_11200"
     CDS             <268..894
                     /locus_tag="PLH31_11200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQR89799.1"
                     /translation="FATGRDPEHAAVAATTGLLAMLDRDEIAGVMAHELAHVKNRDTL
                     TMTVTATIAGAISALANFGLFFGGSRDEDRPGGMIGAIAVAILAPIAAMLVQMAISRS
                     REYEADRIGAQIGGDPAALARALQKIEAYARGVRVNHQAERNPATAHMFIINPLNGQG
                     ADNLFSTHPATHNRVEALLALGVGQGTRPRRVGTAVPVSRPRRPGPWS"
     gene            979..1338
                     /locus_tag="PLH31_11205"
     CDS             979..1338
                     /locus_tag="PLH31_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89800.1"
                     /translation="MTPPTRRSLLVSGVALLAAGCATRPSLVASDQSWPELEAVRITA
                     SRHELTVRVASKGCAAKADFVFHVDRTGRTAVLAFARRRLETCRFGAATQVDLVFSYD
                     ELGLKRGERFAVANPRL"
     gene            complement(1524..1943)
                     /locus_tag="PLH31_11210"
     CDS             complement(1524..1943)
                     /locus_tag="PLH31_11210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR89801.1"
                     /translation="MSRVFVPASNAFSMTFTPKASDIDANEHVNNVVYLAWAQDLAIA
                     HWEAWASAEERAGWSWVARRHEIDYRRELRPDEIATGYTWVGELKGPRFERYVRIDGP
                     DGEMCAQSRTDWVLIDIAARRPARVMDWMVERFSAKA"
     gene            complement(1940..2281)
                     /gene="grxD"
                     /locus_tag="PLH31_11215"
     CDS             complement(1940..2281)
                     /gene="grxD"
                     /locus_tag="PLH31_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQR89802.1"
                     /translation="MTDAAASPALDFIAKTVAENPVVVFMKGVPDQPRCGFSAVSVQI
                     LDHLGVEFIGVDVLQDDELRQGVKTFTDWPTIPQLYVKGEFIGGSDIVREMFQSGELK
                     TLLTEQGLIAA"
BASE COUNT          352 a          779 c          777 g          388 t
ORIGIN      
        1 gctggcgggc ctgacggccc tgttcatggc ggcgggtttc ttgctgggcg gcgccacggg
       61 catgatgatc gccctggtct tcgccatcgg cctgaacgcg ttctcctact ggaacgccga
      121 caagatcgtg ctgaagacct atggcgcggt cgaggtggac gaaacccacc ccgaccgccg
      181 catccgtgac tacgccatcg acgtccacgc catggccgat cgcgccggcc tgccgcgtcc
      241 tcgcgtctgc gtgatggnnn nnnnnnnttc gccacgggcc gcgaccccga gcatgccgcc
      301 gtggccgcca ccacgggcct gctggccatg ctggaccgcg acgagatcgc cggcgtcatg
      361 gcccacgaac tggcccatgt gaagaaccgc gacaccctga ccatgacggt gacggccacc
      421 atcgccggcg ccatctcggc cctggccaat tttggcctgt tctttggcgg ctcgcgcgac
      481 gaggaccggc cgggcggcat gatcggcgcg atcgccgtcg ccatcctggc ccccatcgcc
      541 gccatgctgg tgcagatggc catcagccgc tcgcgcgaat acgaggccga ccgcatcggc
      601 gcccagatcg gcggcgatcc cgccgccctg gcccgcgccc tgcagaagat cgaggcctat
      661 gcccggggcg tgcgggtgaa tcatcaggcc gagcgcaatc cggctacggc tcacatgttc
      721 atcatcaacc cgctgaacgg ccagggcgcc gataacctgt tctcgaccca cccggccacc
      781 cacaaccgcg tcgaggcgct gctggcgctg ggcgtggggc agggcacgcg accccgtcgg
      841 gtcggcacgg ccgtgccggt cagtcgcccg cgtcggccgg gaccctggag ctaggattcc
      901 agtacgaagg ccattgcgct ggcggtgtct gtcctcaagg attggatttc gatccgtctt
      961 tccaggtttg tcccgccgtt gacgccgcca acccgtcgat ccctgctggt tagcggcgtg
     1021 gccctgctcg cggcgggctg cgcgacacgg cccagccttg tggcgtccga ccagagctgg
     1081 cctgaattgg aggctgtgag gatcactgcc agccgtcatg aactcacggt gcgcgtcgcc
     1141 tcaaagggct gcgccgccaa ggccgacttc gtctttcatg ttgatcggac gggcaggacc
     1201 gccgtgctag ccttcgcgcg gcgacgactg gagacgtgtc ggttcggggc ggcgacccag
     1261 gtcgacctgg tgttcagcta tgacgagctt gggctgaagc gcggcgagcg gttcgcggtg
     1321 gccaatcctc gtctgtgaaa actgaggcca gcgccggacg ccccctccgt cacgtcgcgt
     1381 atccgcgccg cgccacctcc cccgtttcac ggggcaggag ggtgaaacgg tttttctcct
     1441 gccccgtagc gaagcgtgcg ggggaggtgg cgcgctgcga atacgcagcg tgacggaggg
     1501 ggcgctgcgg cgttggcgaa agctcaggcc ttcgcgctga accgctcgac catccagtcc
     1561 atcacccggg ccgggcgccg ggcggcgatg tcgatcagca cccagtcggt gcggctctgg
     1621 gcgcacatct cgccgtcggg accgtcgatg cgcacatagc gctcgaagcg cggacccttc
     1681 agttcgccga cccaggtgta gccggtggcg atctcatcgg ggcgcagttc gcggcgatag
     1741 tcgatctcgt ggcggcgggc cacccagctc catccagccc gctcttcggc cgaagcccag
     1801 gcttcccaat gcgcgatggc cagatcctgg gcccaggcca gatagaccac gttgttgacg
     1861 tgctcattgg cgtcgatgtc cgacgccttg ggcgtgaagg tcatcgagaa ggcgtttgag
     1921 gccgggacga agaccctact caagccgcga tgaggccctg ctcggtcagg agggtcttca
     1981 gttcgcccga ctggaacatc tcgcggacga tgtcgctgcc gccgatgaat tcacccttca
     2041 catagagctg gggaatggtg ggccagtcgg tgaaggtctt gacgccctgg cgcagctcgt
     2101 cgtcctgcag cacgtcgacg ccgatgaact cgacgcccag atggtcgagg atctggacgc
     2161 tgaccgccga gaagccgcag cgcggctggt cgggaacgcc cttcatgaac accaccaccg
     2221 gattttcggc gacggtcttg gcgatgaagt ccagggcggg ggaggcggcg gcgtcggtca
     2281 tggcggcaat tccttgggcg taggcc
//
LOCUS       DAOMEO010000495         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4942_length_2304_cov_2.530013, whole genome shotgun sequence.
ACCESSION   DAOMEO010000495 DAOMEO010000000
VERSION     DAOMEO010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_2304_cov_2.530013"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLH31_11220"
     CDS             complement(<1..547)
                     /locus_tag="PLH31_11220"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQR89803.1"
                     /translation="MAYDQTDAMPSLAARLDAELRDAHPLTILHKAHETFGDKLALVS
                     SFGAESAVLLHLASRVSPNIAVLFLDTGMLFGQTLDYRKSLAAQLALTDVRDLRPAFA
                     DLAVNDPKSDLWRTDIDGCCHIRKVLPLDRALNGFDGWITGRKRFHGGDRLRLAVVEE
                     AEGKIKFNPLANWSKAELDAYI"
     gene            complement(547..1053)
                     /locus_tag="PLH31_11225"
     CDS             complement(547..1053)
                     /locus_tag="PLH31_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HQR89804.1"
                     /translation="MPRLIKLENGVAGFADDSFVHVTDEDAIPETGDVILSLARFQAE
                     GDALLSSNSRRVGVRIESNEEVEALAYDLPRLAVVAVAFPKFRDGRAYTSARLLRERF
                     NYQGEVRAVGDVLQEQAGFMVRCGMDAFEPADGAGPEVWTKAANRFRHVYQRAADARP
                     VAFEERSA"
     gene            complement(1037..>2304)
                     /locus_tag="PLH31_11230"
     CDS             complement(1037..>2304)
                     /locus_tag="PLH31_11230"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HQR89805.1"
                     /translation="AGATAEEIDDPRVWAEVIRQWSTLHPEFSYLPRKFKIAITGAAK
                     DRTAAKVHDIGLMLRRARDGSLGFEVIVGGGQGRTPYVGPTIKEHLPVSRLLSYLEAV
                     LRVYNRHGRRDNIYKARIKILVAALGAEEFGRQVEEEWAKIDAARADLPDHELARIRG
                     AFASTPFETLPERSDVFEAARAASPVLQRFARNNIKPHKKPGYAIVEISLKAQGQTPG
                     DATADQLDLIADLAEQYSLNDVRVTHAQNMVLPHVKLDDVPAVHAALAAAGLATANID
                     LASDIIACPGLDYCALANARAIGVAQDIAAKFADEDHAEKVGELKIKISGCINACGHH
                     HVAHIGILGVDKKGEEFYQLSLGGSGAEDASIGKILGPALPADKVAGAIDTLVEAYLG
                     IRTGEERFLDTYRRVGLEPFKEAVYAQAD"
BASE COUNT          372 a          748 c          794 g          390 t
ORIGIN      
        1 cgatataggc gtccagctcg gccttggacc agttggccag cgggttgaac ttgatcttgc
       61 cctcggcttc ctcgaccacc gccagccgca gacgatcgcc gccgtggaag cgcttgcggc
      121 cggtgatcca gccgtcaaaa ccgttcagag cacggtccag gggcagcacc ttgcggatat
      181 ggcagcagcc gtcgatgtct gtgcgccaca ggtcggactt cgggtcattg acggccaggt
      241 cggcgaaggc cgggcgcaga tcgcgcacat cggtcagggc caactgggcg gccagtgact
      301 tgcgatagtc cagggtctgg ccaaacagca tgccggtatc gaggaacaat accgcgatat
      361 tgggcgacac ccgcgaggcc aggtgcagca gcacggctga ttccgcccca aaggacgaca
      421 ccagggccag cttgtcgccg aaggtctcat gggccttgtg caggatggtc agcggatggg
      481 cgtcgcgcag ctcggcgtcc agccgggccg ccaggctggg catcgcgtcc gtctggtcgt
      541 aagccatcac gcgcttcttt cctcgaacgc caccggccgg gcgtcggcgg cgcgctgata
      601 gacgtggcgg aagcggttgg cggccttggt ccagacctcg gggcctgcgc catcggccgg
      661 ctcgaaggcg tccatgccgc agcgaaccat aaagccggcc tgctcctgca acacgtcgcc
      721 caccgcccgc acttcgccct gatagttgaa gcgctcacgc agcagacggg ccgaggtata
      781 ggcgcggccg tcgcggaact tcggaaaggc cacggccacc accgccagac gcggcaggtc
      841 ataggccagg gcctcgacct cttcgttgga ctcgatgcgc acccccaccc tgcggctgtt
      901 ggacgacagc agcgcgtcgc cctcggcctg gaagcgggcc aaggacagga tgacgtcgcc
      961 ggtttccggg atggcgtcct cgtcagtcac atggacgaag ctgtcgtcgg cgaaaccggc
     1021 gacgccattc tcgagcttaa tcagcctggg catagacagc ctccttgaag ggctcgaggc
     1081 cgacgcggcg ataggtgtcg aggaagcgct cctcgcccgt gcggataccc agataggcct
     1141 cgaccagggt gtcgatggcg ccggccacct tgtcggcggg cagcgccggg cccaggatct
     1201 taccgatcga ggcgtcttcg gcgccggaac cgcccagcga cagctggtag aattcctcgc
     1261 ccttcttatc gacgcccagg atgccgatgt gggccacgtg atggtggccg caggcgttga
     1321 tgcagccgct gatcttgatc ttcagttcgc ccaccttttc ggcgtggtcc tcgtcggcga
     1381 acttggcggc gatatcctgg gccaccccga tggcgcgggc attggccagg gcgcaatagt
     1441 ccaggcccgg gcaggcgatg atgtcgctgg ccagatcgat attggcggtg gcgagccccg
     1501 ccgccgcgag cgcggcgtgg acagccggaa catcatccag cttcacatgc ggcagcacca
     1561 tgttctgggc gtgggtcacc cgaacatcgt tgaggctgta ctgctcggcc agatcggcga
     1621 tcaggtccag ctggtcggcc gtcgcgtcgc ccggcgtctg gccttgcgcc ttcagcgaga
     1681 tctcgacaat ggcgtagccg ggcttcttgt gtggcttgat gttgttgcgc gcgaagcgtt
     1741 gcaggaccgg gctggcggcc ctggcggctt caaagacgtc cgagcgctcg ggcagagtct
     1801 cgaacggcgt cgaggcgaag gcgccccgga tgcgggccag ttcgtggtcg ggcaggtcgg
     1861 cgcgggcggc gtcgatcttg gcccactctt cctccacctg acggccgaac tcctcggcgc
     1921 ccagggcggc gactaggatc ttgatgcggg ccttgtagat gttgtcgcgg cgaccatggc
     1981 ggttatagac ccgcagcaca gcctccagat agctgagcag acggctgacc ggcaggtgct
     2041 ccttgatggt ggggccgaca tagggcgtgc gaccctggcc gccgccgacg attacctcga
     2101 aacccagcga gccgtcgcgg gcccggcgca gcatcaggcc gatatcgtgc accttggcgg
     2161 ccgtgcggtc cttggcggcg ccggtgatgg cgatcttgaa cttgcgcggg agatagctga
     2221 actcagggtg cagggtcgac cactggcgga tcacctcggc ccagacgcgc gggtcgtcga
     2281 tctcctcggc cgtggcgccg gcat
//
LOCUS       DAOMEO010000496         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4943_length_2304_cov_2.480213, whole genome shotgun sequence.
ACCESSION   DAOMEO010000496 DAOMEO010000000
VERSION     DAOMEO010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4943_length_2304_cov_2.480213"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLH31_11235"
     CDS             <1..462
                     /locus_tag="PLH31_11235"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQR89806.1"
                     /translation="IDSAFDAMVEACAASRPGRLETWINAPIQQLYGELFARGLAHSV
                     ECWRDGQMVGGLYGVSLGAAFFGESMFSTERDASKVALAHLVGRLRIGQYRLLDTQFI
                     TDHLLQFGALEISRADYRRRLAKAWPVQADFYRLTAGATGADVLQAISHAS"
     gene            complement(399..710)
                     /locus_tag="PLH31_11240"
     CDS             complement(399..710)
                     /locus_tag="PLH31_11240"
                     /inference="COORDINATES: protein motif:HMM:NF021444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HQR89807.1"
                     /translation="MTTETMRFEAPVGQPIRYKTLIFTVRACETSAADEVAPESTAYI
                     VVDTQPKAVAGRVAPPGRQVYKGWMYANSPGMNPLQHPVYDAWLIACKTSAPVAPAVK
                     R"
     gene            complement(1040..1435)
                     /locus_tag="PLH31_11245"
     CDS             complement(1040..1435)
                     /locus_tag="PLH31_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HQR89808.1"
                     /translation="MLKAIFTWWNGATLGQRFHIGRRGVFIGQDDFGNRYFEARDASD
                     SYDAHKRRWVIYDGYAEASKVPAEWSGWLHYTFDEPPTTNPLPRRSWEKDHIPNLTGT
                     VHAWRPKGSLARGGERALATSDYQPWKPE"
     gene            1654..>2304
                     /locus_tag="PLH31_11250"
     CDS             1654..>2304
                     /locus_tag="PLH31_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase"
                     /protein_id="HQR89809.1"
                     /translation="MRSQSGFIDQPVGVERRDLERSNAIVEVMAPPTWTDARVEAWLD
                     WAGETLEPDAPLGGGPARYADRLARAGLEKGLFADAADAAAFNNALLATMLTGVATPA
                     GAFSSLDLLPDIAEIEFRQVIESQLSRRRSHALASKAAARLDTALAQVSDAVQRCHGD
                     AKACSDPRKNSALARAARRARDLGADDRMISDAIALVGAPRTPLIDSIAAPATAVVA"
BASE COUNT          371 a          753 c          768 g          412 t
ORIGIN      
        1 atcgatagcg ccttcgacgc catggtcgag gcttgcgcgg cctcccgccc cggccgactg
       61 gaaacctgga tcaacgcgcc gatccagcag ctttatggcg agctcttcgc gcgcggcctc
      121 gcccattcgg ttgaatgctg gcgcgatggc cagatggtcg gcgggcttta tggcgtctca
      181 ctaggcgctg cattctttgg cgagagcatg ttctcgaccg agcgtgacgc cagcaaggtg
      241 gcgctggccc atctggttgg gcggctgagg atcggccagt atcgactgct cgacacacag
      301 ttcatcaccg accatctgct acagttcggg gcgctcgaaa tctcgcgggc agattacagg
      361 cgtcggctgg ccaaggcctg gccggtccag gcggacttct accgcttaac ggcgggtgcg
      421 acgggcgcgg acgtcttgca ggcgatcagc catgcgtcat agaccgggtg ctgcagcggg
      481 ttcatgcccg gcgagttggc gtacatccag cccttgtaga cttgacgccc cggaggagcg
      541 acccggccag cgaccgcctt gggttgggtg tcgaccacga tgtaggccgt agactctggc
      601 gcaacttcat ccgcagcaga ggtctcgcag gcacggaccg tgaagatcag ggtcttgtag
      661 cgaatgggct gaccgaccgg cgcctcgaag cgcatcgtct cggtcgtcac cttgtccagc
      721 gcttggataa tggcgacgga cgagcgaccg cgcttagccg gttcggcagg ttttgcaggt
      781 gtcgcagtct tggcctgagc cggcttggcg gcaggtttct cggccacggg ttcgggtagg
      841 agcgcgggcg tcgcagcgcg aggttgcggg gccggcgtcg cgacgcgcgg ttgcggcggc
      901 tcaggcgtcg cagctaccgg ggcggtaagc gcttgtggag ccaccggcgc cgtcgactga
      961 cgcgcagagg cgccgcccat catcgtcagg ccgattgcca gcaccgacgc accaagggcc
     1021 gctaagcgag gcatcaaacc tattccggct tccagggctg ataatcgctg gtcgctaaag
     1081 cgcgttcgcc gccgcgcgcc agggagccct tcggacgcca agcgtgaacc gtgccggtca
     1141 gattgggaat atgatccttt tcccaactcc ggcggggcag tgggttcgtg gtcggcggct
     1201 cgtcgaaggt gtagtgcaac cagccgctcc actctgccgg aaccttggag gcctcggcat
     1261 agccgtcata gatcacccag cggcgcttgt gggcgtcgta gctgtcgctg gcgtcgcggg
     1321 cttcgaaata gcggttgccg aaatcgtcct ggccgatgaa cacgccgcgg cggccaatgt
     1381 ggaaccgctg acccagggtc gcgccgttcc accacgtgaa gatcgctttc agcactacgt
     1441 ttgcacccga atttgcaagc acttcccggt cgcgacgagc gcgacgaagc ggccggacta
     1501 tagggcggcc ggcggtaggc gtccagcatc cgccatccac gtctcaattc aacatcttgt
     1561 ggagcatgcg gtccgagccc ccaacatcta ttgaagcgag gttggtcgca atctaggttg
     1621 gaactctcgt ggcgtcttga cgcggaatcg cccatgcgca gtcagtccgg atttatcgat
     1681 cagcccgtcg gggtagagcg acgcgacctt gagcgcagca acgccatcgt cgaagtcatg
     1741 gctccgccga cctggacgga cgcgcgtgtt gaagcctggc tggactgggc tggcgagact
     1801 ctggagcccg acgcgccgct cggcggcggt ccggcccgtt atgccgaccg cttggccagg
     1861 gccggcctgg agaagggtct gttcgccgat gccgccgatg cggccgcctt caacaacgct
     1921 ttgcttgcca cgatgctgac gggggtcgcc acgcccgccg gcgctttctc ctcgctggat
     1981 ctcctgccag atatcgccga gatcgagttc cgccaggtca ttgaaagcca actgagccgt
     2041 cgtcgcagcc atgccctggc cagcaaggcc gccgcccggc tcgacaccgc cctggcccag
     2101 gtttccgacg ctgtgcagcg ctgccatggt gacgccaagg cctgctccga tccccgcaag
     2161 aacagcgccc tggcccgcgc cgcccgtcgc gctcgcgatc tgggcgccga tgaccggatg
     2221 atctcggacg ccatcgccct ggttggcgcg ccgcgcacac cgctgatcga cagtatagcg
     2281 gcgcccgcaa cggccgtggt cgcc
//
LOCUS       DAOMEO010000497         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4946_length_2303_cov_2.297153, whole genome shotgun sequence.
ACCESSION   DAOMEO010000497 DAOMEO010000000
VERSION     DAOMEO010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4946_length_2303_cov_2.297153"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLH31_11255"
     CDS             <1..442
                     /locus_tag="PLH31_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89810.1"
                     /translation="GTGGGLAGAGLGAAIGGSVSGNGAKTEGAVLGGLLGAVIGSSIG
                     KNSAACTPGATPPPPPPPTAYNNYDNRYDARPYDDPRYEDARRDAERDAYSRESYSYR
                     VDQRSADVNGCTLAESPIYLPDGRVQKRFVRVCPDAQGRFQVVD"
     gene            complement(511..1242)
                     /locus_tag="PLH31_11260"
     CDS             complement(511..1242)
                     /locus_tag="PLH31_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQR89811.1"
                     /translation="MTRRRVDVLMMERGLFDSRAKAREAIEAGLVRADGVLVLKPSEM
                     VEEHVSIRASLAHPWVGRGALKLVHALDLWPIEVEGKVAIDVGASTGGFTEVLLSRGA
                     AKVYAVDVGREQLHHSLVEHERVVDLSGTDARILDTSWIEEDPQLIVSDVSFISMTKA
                     LPAVLALAPSGADLITLVKPQFEAGKGHVGKNGLVKDPQTIARVRREIVDFLEEAGWT
                     VMGEDESPITGGDGQVESLVWARRR"
     gene            1343..>2061
                     /locus_tag="PLH31_11265"
     CDS             1343..>2061
                     /locus_tag="PLH31_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQR89812.1"
                     /translation="MHFSRRGLLAASAAIAAASSVEAKPRAAGEPAWTPDATEIAGRI
                     RRKEVSVVEVVEEAIRKAEALQPRINALVTSDFDRAMDKARGGQLSGPFAGVPFLIKD
                     LDPYTGLPTRYGSRSSMGAGPETAQTAFVETYDRAGLVVIGKSTTPEYGFLPTTEPMG
                     FLPTRNPWNLKHSSGGSSGGAAAATAAGIVPFAHASDGGGSIRIPAANCGLFGLKPSR
                     GRMIEGRPHGRGIDLSVAHVV"
     assembly_gap    2062..2071
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2072..>2303
                     /locus_tag="PLH31_11270"
     CDS             <2072..>2303
                     /locus_tag="PLH31_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-aminohexanoate hydrolase"
                     /protein_id="HQR89813.1"
                     /translation="SHSVRDSAAMFGASERTAAGSPFKAIGVVTKPLKKRLKVGLLLE
                     TGMGQKPDAEVVAAIEGAAKLMASLGHRVEPTS"
BASE COUNT          375 a          828 c          741 g          349 t
ORIGIN      
        1 cggcacgggc ggcggtctgg ccggcgcggg cctgggcgcg gccatcggcg gcagcgtctc
       61 gggcaatggc gccaagaccg aaggcgccgt cctgggcggc ctgctggggg cggtgatcgg
      121 cagcagcatc ggcaagaact cggcggcctg caccccgggc gcaacgcccc cgccgcctcc
      181 cccgccgacc gcctataaca attacgacaa tcgctatgac gcgcggccct atgacgatcc
      241 tcgctatgag gacgcccgcc gcgacgccga gcgcgacgcc tatagccgtg aatcgtattc
      301 ctaccgcgtc gaccagcgct cggccgacgt caatggctgc accctggccg agagcccgat
      361 ctacctgcct gacgggcgcg tccagaagcg cttcgtgcgg gtctgccccg acgcccaggg
      421 tcgctttcag gtcgttgact gagcttccga ccagtggctt gagctagccc cagatcagac
      481 ggccgctcgc ctcgcgtgag cggccgtttt ctatcggcgg cgcgcccaga ccagactctc
      541 gacctgacca tcgccgccgg tgatcgggct ttcatcctcg cccatcacgg tccagccggc
      601 ctcttccagg aaatccacga tctcgcggcg cacgcgggcg atggtctgtg gatccttgac
      661 cagcccgttc ttgcccacat ggcccttgcc cgcctcgaac tgcggcttga ccagggtgat
      721 caggtcagcg cccgacgggg ccagcgccag cacggccggc aacgccttgg tcatcgagat
      781 gaagctgacg tcgctgacga tcagttgcgg atcctcctcg atccagctgg tgtcgaggat
      841 gcgcgcatcg gtgccggaca gatccacgac ccgctcgtgc tcgaccaggc tgtggtgaag
      901 ctgctcgcgc ccgacatcga ccgcatagac cttggccgcg ccgcgcgaca acagcacctc
      961 ggtaaagccc ccggtcgagg cgccgacatc gatggcgacc ttgccctcga cctcgatggg
     1021 ccacagatcc agggcgtgga ccagcttcag cgcgccgcgc ccgacccagg ggtgggccag
     1081 cgaggcgcga atcgaaacgt gctcttccac catctccgac ggcttgagga ccagcacgcc
     1141 atcggcgcgc accagcccgg cctctatggc ctcgcgagcc ttggcgcggc tgtcgaacag
     1201 gccgcgctcc atcatcaaca catcaacacg tctacgggtc atgggcgcgg tctagcatag
     1261 acttgcagcc aaggccgctt caggttcaac tgaacgccgt tcaccggcgc gccaagacgc
     1321 cggtcgctcg ggagggattt ccatgcactt cagtcgccgc ggcctgctgg ccgcctctgc
     1381 cgcgatcgcc gccgcttcgt ccgtcgaagc caagcccagg gccgccggtg agccggcctg
     1441 gacgcccgac gccaccgaga tcgccggccg catccgtcgc aaggaggtca gtgtcgtcga
     1501 ggtggtcgaa gaggccatcc gcaaggccga ggccctgcag cccaggatca acgccctggt
     1561 aacctccgat ttcgaccggg cgatggacaa ggcccggggc ggccagctgt cgggcccgtt
     1621 cgccggcgtg ccgttcctga tcaaggacct ggatccctat actggactgc ccacgcgcta
     1681 cggctcccgc agttcgatgg gcgcaggccc agaaacggcc cagacggcct ttgtcgagac
     1741 ctatgatcgc gccggtctgg tggtgatcgg caagtcgacc acgccggaat acggcttcct
     1801 gccaacgacc gagcccatgg gcttcctgcc cacccgcaat ccctggaacc tgaagcactc
     1861 gtcgggtggc tcatccggcg gcgcggcggc cgcgacggcg gcgggcatcg tgcccttcgc
     1921 tcacgccagc gacgggggcg gctcgatccg cattccagcg gccaattgcg gcctgtttgg
     1981 cctgaaacct tcacgcggcc ggatgatcga gggccggcct cacgggcgtg gcatcgacct
     2041 gtccgtggcc catgtggtca gnnnnnnnnn ncagccacag cgtccgcgat tcggcggcca
     2101 tgttcggcgc cagcgagcgc acggcggcgg ggtcgccgtt caaggccatc ggcgtagtca
     2161 ccaagccgct gaaaaagcgc ctcaaggtcg gcctgctcct ggaaaccggc atgggccaaa
     2221 agccagacgc cgaggttgtc gccgccatcg agggcgccgc caagctgatg gccagcctgg
     2281 gacacagggt cgagccgaca agc
//
LOCUS       DAOMEO010000498         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4948_length_2303_cov_1.862100, whole genome shotgun sequence.
ACCESSION   DAOMEO010000498 DAOMEO010000000
VERSION     DAOMEO010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_2303_cov_1.862100"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>448)
                     /locus_tag="PLH31_11275"
     CDS             complement(<1..>448)
                     /locus_tag="PLH31_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR89814.1"
                     /translation="GRYGGEIYPLTRVLLMPLMGSFGLIPMIIAIYYSGELVWRERER
                     KTHEIIDATPIPDWAFVVPKTLAISLVLISTLLVSVLAAIISQLAHGYTHLELGKYLL
                     WYVLPQSVDWILLAVLAVFLQAVSPHKFVGWGLMVIYMVATITFTNL"
     assembly_gap    449..458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<459..>1371)
                     /locus_tag="PLH31_11280"
     CDS             complement(<459..>1371)
                     /locus_tag="PLH31_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR89815.1"
                     /translation="LLTFGAATIDQIRIGGGGQIHKNAPYAIAQTHLILSIFYMFVTT
                     AFVANVVVRDDETGFGPILRSTRIGKFDYLYGRFTGAFLAAAISFLVVPLAIFIGSFM
                     PWIDPERLGPNLLDAYLFSYFALALPSILLTSAIFFALATVTRSMMWTYVGVIAFMVV
                     WIIAGIALDRPEFERGAALWEPLGTAAFGLATKYWTATERNTLTPALTGALLFNRVFA
                     VALSAAFLGAAYWLFKIQSAELAGDRKVKAKAGPAPVEILPAAPSGPIVPRHDRQTAW
                     AQLRVRTRLDMGQVFKSPAYFVLLALGL"
     assembly_gap    1372..1381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1382..1459)
                     /locus_tag="PLH31_11285"
     CDS             complement(<1382..1459)
                     /locus_tag="PLH31_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89816.1"
                     /translation="MFAKIAGFELRYQLKSPVFWVVATVF"
     gene            complement(1471..2259)
                     /locus_tag="PLH31_11290"
     CDS             complement(1471..2259)
                     /locus_tag="PLH31_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89817.1"
                     /translation="MLIIENLTHVYGNGVRALDEVSLTIPRGMYGLLGPNGAGKSTLM
                     RTIATLQAPTSGHIRFDDIDVLKTPELLRRTLGYLPQDFGVYPRVSAYDMLDHMAVLK
                     GIAGAKARKETVESLLNQVNLWGVRKKALAGFSGGMRQRFGIAQAEIGENVVVILSTH
                     IVEDVSDLCPAMAIICDGRIVKQGAPNDLVVQLAGRVWRKTIDKADLEAAKAQYQVIS
                     TRLLGGRTVIHILADSDPGEGFAAVEGGLEDVYFSTLSTTRRAA"
BASE COUNT          450 a          721 c          769 g          343 t
ORIGIN      
        1 caggttcgtg aacgtgatgg tggcgaccat gtagatcacc atcaggcccc agccgacgaa
       61 cttgtgcgga ctgaccgcct gcaggaacac cgccagcaca gccagcagga tccagtccac
      121 cgattgcggc agcacgtacc agagcaggta cttgcccagc tccagatggg tgtagccgtg
      181 ggccagctgg ctgatgatcg ccgccagcac gctgaccagc agggtcgaga tcaggaccag
      241 cgagatggcc agggtcttgg ggacgacaaa cgcccagtct ggaatcgggg tggcgtcgat
      301 gatctcgtgg gtcttgcgct cgcgttcacg ccagaccagc tcgccggaat aatagatggc
      361 gatgatcatc gggatcaggc caaacgaccc catcagcggc atcagcagca cgcgggtgag
      421 cggatagatc tccccaccat agcgaccgnn nnnnnnnnag gcccagggcc agcagcacga
      481 aataggccgg gctcttgaac acctggccca tgtccagccg ggtgcggacc cttagttggg
      541 cccaggccgt ctggcggtca tgacgtggaa cgatgggacc gctgggcgca gccggcagga
      601 tctcgacggg ggcgggaccg gccttggcct tgaccttgcg gtcgccggcc agctcggccg
      661 actggatctt gaacagccag taggccgctc ccaggaaagc cgccgacagg gccacggcga
      721 agacgcggtt gaacagcagg gcgccggtca gggcgggggt gagggtgttg cgctcggtgg
      781 ccgtccagta cttggtggcc aggccgaagg ccgccgtgcc caggggctcc cacagggccg
      841 cgccgcgctc gaactcggga cggtccaggg cgatgccggc gatgatccag accaccatga
      901 aggcgatgac gcccacatag gtccacatca tcgagcgggt caccgtggcc agagcaaaga
      961 agatcgccga ggtcagcagg atcgagggca gggccagggc gaaataggaa aacagatagg
     1021 cgtccagcag gttggggccc agccgctcgg ggtcgatcca cggcatgaac gagccgatga
     1081 agatcgccag gggcacgacc aggaacgaga tcgcggccgc cagaaacgcg cccgtaaagc
     1141 ggccgtacag atagtcgaac ttgccgatcc gggtggagcg caggatcggc ccaaagcccg
     1201 tctcgtcgtc gcgcaccacc acattggcca cgaaggccgt ggtcacgaac atgtagaaga
     1261 tcgacaggat caggtgggtc tgagcgatcg catagggcgc gttcttgtgg atctgaccgc
     1321 cgccgccgat gcggatctgg tcgatcgtcg ccgccccgaa ggtcagcagg annnnnnnnn
     1381 ngaataccgt cgccaccacc cagaacacag gcgacttcag ctggtagcgg agctcgaagc
     1441 ccgcgatctt ggcgaacatg ggctgtcctc ctacgccgcg cggcgggtgg tggacagggt
     1501 cgagaaatag acgtcctcca gaccgccctc gacggcggcg aagccctcgc caggatcgct
     1561 gtcggccagg atgtggatca ccgtgcggcc gcccagcagg cgggtggaga tcacctgata
     1621 ctgcgccttg gccgcttcga gatcggcctt gtcgatggtc ttgcgccaga cccggccggc
     1681 cagctggacc accagatcat tgggggcgcc ctgcttgacg atgcggccat cgcagatgat
     1741 cgccatggcc ggacacaggt ccgacacgtc ctcgacgatg tgggtcgaca ggatcaccac
     1801 cacgttctcg ccgatctcgg cctgggcgat gccgaagcgc tgacgcatgc cgcccgagaa
     1861 cccggccagg gccttcttgc gcacgcccca cagattgacc tgattgagca ggctctcgac
     1921 ggtttccttg cgcgccttgg cgccagcaat gcccttgagc accgccatgt ggtcgagcat
     1981 gtcgtaggcc gagacgcgcg gatagacgcc gaaatcctgc ggcagatagc ccagggtgcg
     2041 gcgcagaagc tccggggtct tcagcacgtc gatatcgtcg aaacggatat ggcccgaagt
     2101 cggggcctgc agggtggcga tggtgcgcat cagggtcgac ttgcccgcgc cgttcggacc
     2161 cagcaggcca tacatgccgc gcggtatggt caggctgacc tcgtccaggg cccgaacgcc
     2221 gttgccgtag acgtgagtca ggttctcgat gatcagcatt cgcgcgctca cagcccccgc
     2281 ttggaaagac gcccaatatt ccc
//
LOCUS       DAOMEO010000499         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4953_length_2302_cov_2.417445, whole genome shotgun sequence.
ACCESSION   DAOMEO010000499 DAOMEO010000000
VERSION     DAOMEO010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_2302_cov_2.417445"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLH31_11295"
     CDS             complement(<1..691)
                     /locus_tag="PLH31_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR89818.1"
                     /translation="MTHRRHVLMLAAALCLAAPTAFAADAALKAAVAAPHRPAADVAR
                     DGARHPYESLSFWGLKPKQTVIEISPGNGYWTEILAPYAKATGGTYIAGVADLANPKL
                     SDGARKGRADFEARFADEARYGKVGYANFGPVSGPLGAPGSADLVLTARNVHNWTGQP
                     GVAEKAFADFFAVLKPGGLLAVEDHRADPRPEGSPGSGGYLATATVVTLAEKAGFKLD
                     ATSEINANPKDT"
     gene            923..1393
                     /locus_tag="PLH31_11300"
     CDS             923..1393
                     /locus_tag="PLH31_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HQR89819.1"
                     /translation="MRTIDLSPLYRSLVGFDRLAAQLDAAANNEAASGYPPYNIERTD
                     ENSYRIEIAVAGFKSDELSIEVKENLLTVTGRKTANDDVKRYLHRGLAERNFERRFQL
                     ADYVIVTDAALADGLLSISLKRELPEALKPRTIEIKSDSAPLIEGKKVKSEKAA"
     gene            complement(1473..>2031)
                     /locus_tag="PLH31_11305"
     CDS             complement(1473..>2031)
                     /locus_tag="PLH31_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR89820.1"
                     /translation="AVTAQALAKAQELHQRALENQRWCQTGGKEGQCARLDGEDLRPL
                     GDRLKGLRLTAMSAKGACMVGLDLSGAQLQGANLQNADLRMANLRGADLRGARLAGAN
                     LTKADLRQAFLCPLPLGPERRTVANLASARLRYAMIQMADLTEAVFDGADLRGADFTG
                     ARAAKASFRECDLSQAHGLDLSAPE"
     assembly_gap    2032..2041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2042..>2302)
                     /locus_tag="PLH31_11310"
     CDS             complement(<2042..>2302)
                     /locus_tag="PLH31_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR89821.1"
                     /translation="TRQGEVDEVNFSGATLDGSQFEGVSAFKADFSDCSLRGAKLSGA
                     NLKEANLSGAILDGADIKGANLEGANLQGSVMAGVDISTART"
BASE COUNT          388 a          765 c          762 g          377 t
ORIGIN      
        1 tggtgtcctt ggggttggcg ttgatctcgg aggtggcgtc cagtttgaag ccagccttct
       61 cggccagtgt gaccacagtg gcggtggcca gatagccgcc ggaacctggg ctgccctcag
      121 ggcggggatc ggcgcggtga tcctcgacgg ccagcaggcc gcccggcttc agcacggcga
      181 agaaatcggc aaacgccttc tcggcgacgc ccggctggcc ggtccagttg tggacgttgc
      241 gagcggtcag caccagatcg gccgaacccg gcgcacccag cggacccgag accgggccga
      301 aattggcgta gccgaccttg ccatagcgcg cctcatcggc gaatcgcgcc tcgaaatcgg
      361 cgcggccctt gcgggcgccg tccgacagct tggggttggc caggtcggcg acaccggcga
      421 tgtaggttcc accggtggct ttggcgtagg gcgccaggat ctcggtccag tagccattgc
      481 ccggcgagat ctcgatcacg gtctgcttgg gcttgagccc ccagaacgac aggctctcat
      541 agggatggcg ggcgccatcg cgggcgacat cggcggcggg acgatgcggg gcggcgacgg
      601 cggccttgag cgcagcgtcg gcggcgaagg cggtgggcgc ggccaggcag agggcggcgg
      661 ccagcatcag aacatggcga cggtgggtca tcttcgggtc tctccgcgtc aggagcggcg
      721 accttagccg ggggaccgtg acgatcaagc ctttagcgac cgaggttcgc ttcaacctct
      781 tgaagtcgga aattggcgct cccacctgtc taatcgaagg cgctggatac ccgggccttc
      841 cggaccacag gcgacgccga tttcgggccg ccgacgggac gaacacacac ggcaaccttg
      901 cttgataaga aggatgtgaa ccatgcgtac gatcgacctt tcccccctgt accgctcgct
      961 cgtcggcttt gaccgtctgg ccgcgcaact cgacgccgcc gccaataacg aggccgcctc
     1021 gggctatccg ccctacaata tcgagcggac tgacgagaat tcctaccgca tcgagatcgc
     1081 cgtcgccggc ttcaagtcgg acgaactgag catcgaggtg aaggaaaacc tgctcaccgt
     1141 caccggtcgc aagaccgcca atgacgacgt aaagcgctac ctgcatcgtg gcctggccga
     1201 gcgcaatttc gagcgccgct tccagctggc cgattacgtg atcgtcaccg acgcggccct
     1261 ggccgacggc ctgctgtcaa tctcgctgaa gcgcgaactg cctgaagccc tcaagccccg
     1321 gaccatcgag atcaagtcgg actcggcgcc gctgatcgaa ggcaagaagg tcaaaagcga
     1381 aaaggccgcc tgatcggcgg ccctgaacac cgtttaatct cccccctcga cactggcggc
     1441 gcagcgatgc gccgcctttt tcgtccgggg acctattcgg gcgccgagag gtccagaccg
     1501 tgagcctggc tcaggtcgca ttcgcggaag ctggccttgg cggcccgcgc gccggtgaag
     1561 tcggcgccgc gcagatccgc gccgtcgaac acggcttcag taagatcagc catctggatc
     1621 atcgcatagc gcagtcgcgc cgacgccagg ttggcgacgg tgcggcgctc ggggcccagt
     1681 ggcagcgggc agaggaacgc ctggcgcaga tccgccttgg tcagattggc gccggccagc
     1741 ctggcgccgc gcagatcggc gccccgcaga ttggccatgc gcaggtcggc gttctgcaga
     1801 ttggcgccct gtagctgcgc gcccgacagg tccaggccca ccatgcacgc gcccttggcg
     1861 ctcatcgccg tgaggcgcag gcccttgaga cgatcaccca gcggacgcag atcctcgcca
     1921 tccagccgcg cacactggcc ttccttgccg ccggtctgac accagcgctg gttttcgagg
     1981 gcgcgttgat gcagctcttg ggccttggcc agcgcctggg ccgttacggc cnnnnnnnnn
     2041 naggtgcggg cggttgagat gtcgaccccg gccatcaccg agccctgcag gttggcgcct
     2101 tccagattgg cgcccttgat gtcggcccca tccaggatgg cgcccgacag attggcctcc
     2161 ttgagattgg cgcctgacag cttggccccg cgcagggagc agtcgctgaa atcggccttg
     2221 aacgccgaca cgccttcgaa ctgtgagccg tccagcgtgg cgccggagaa attgacctca
     2281 tcgacctcgc cctggcgcgt ct
//
LOCUS       DAOMEO010000500         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4962_length_2297_cov_2.469224, whole genome shotgun sequence.
ACCESSION   DAOMEO010000500 DAOMEO010000000
VERSION     DAOMEO010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4962_length_2297_cov_2.469224"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLH31_11315"
     CDS             complement(<1..255)
                     /locus_tag="PLH31_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89822.1"
                     /translation="MRRLEGKIAIVTGGAGGIGSATARRLTSEGATTVIADLDLERAR
                     VVAAEIGHGATAFHYDAESVDTVEALVASTVARFGRLDILL"
     gene            complement(284..1111)
                     /locus_tag="PLH31_11320"
     CDS             complement(284..1111)
                     /locus_tag="PLH31_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89823.1"
                     /translation="MTDDSRRDPTFVSKYGPWVLVSGASEGTGAEMALLAAAKGCNVI
                     LVARRMEKLEALAATLRQTYGVETRTRSLDLAGPDAAAQLEACADGLDLGLVIFNAGG
                     DSVGGRFLDSSYAQWRTLLQRNIDTLTDACHRFATVLVERGKGGMILIGSEAAFQGTG
                     RLALYSATKAYALNLGESLWRELKPRGVDVLNVLIGATDTPKLRTVFARNNLSPDAVP
                     LTLAEDVARVALDHLGDGPTVVLSALENDDNPMLSRRLRRERVIYHSEFLNHFYGPE"
     gene            complement(1202..>1943)
                     /locus_tag="PLH31_11325"
     CDS             complement(1202..>1943)
                     /locus_tag="PLH31_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89824.1"
                     /translation="LYATTRKGYNAGGFNPNINDPDLEVVRPEFITDYEAGLKADWSL
                     AGMPVRTNLSTFYAKYKDIQRTTSLVFDNLIVTGNFNAAEATIYGGQIEVLARPVEPL
                     TLQASYGYLHTKYDSFQNALLGDVTGNKFAQAPKDTLNVSATYRHALPSGELVANVSY
                     AHISKVAFADDNLTTPGNVAPGYGLVDARLDWKKVGGGNVDLGVYVKNATDEEYLLNT
                     TDRTSRFGFDSRLYGDPRTYGVEIRYAF"
     assembly_gap    1944..1953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1954..>2297)
                     /locus_tag="PLH31_11330"
     CDS             complement(<1954..>2297)
                     /locus_tag="PLH31_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89825.1"
                     /translation="GLYSSASDVDNFNSYLLFAPVGTPHNNNTTQQTGGNTTTDSRAV
                     YAQGTYAVTDRLNLTAGARYTEDDVKTAQHGYSPGLKCNLPAALPSVNIATCTQKIAA
                     KTDALTYNLSVD"
BASE COUNT          369 a          724 c          768 g          426 t
ORIGIN      
        1 cagcaggatg tcgaggcgac caaaccgcgc caccgtggat gcgaccaggg cctcgacggt
       61 gtccacggac tccgcgtcgt agtggaaggc cgtcgcgccg tgtccgatct cagccgcgac
      121 gacgcgcgcg cgttccagat cgaggtcggc gatgaccgtc gtggcgccct cggacgtcag
      181 ccgtcgggcc gtggccgagc cgataccgcc cgcgccgccc gtgacgatcg cgatcttgcc
      241 ttccagccgc ctcatgctcg atctcccacc gtcatgcccg tgctcattcc gggccgtaga
      301 aatggttcag gaattcgctg tgatagatca cgcgttcgcg ccgcagccgg cgcgacagca
      361 tcggattgtc gtcgttctcc agcgcgctga gcacgaccgt ggggccgtcg ccgaggtggt
      421 cgagcgcgac gcgggccaca tcctccgcca gcgtcaacgg cacggcgtcc ggcgacaggt
      481 tgttgcgggc gaataccgtc cgcagcttcg gcgtgtccgt cgcgccgatc aggacgttga
      541 gcacatcgac gcctctcggc ttcagttcgc gccacaggct ttcgcccagg ttcagcgcat
      601 aggccttggt ggccgaatag agcgccagcc gccccgtgcc ctggaacgcc gcctccgagc
      661 cgatcaggat catcccgccc ttgccgcgtt cgaccaggac cgtggcgaac cggtggcagg
      721 cgtcggtcag cgtgtcgatg ttccgctgca agagcgtgcg ccattgggca taggagctgt
      781 ccaggaaacg cccgccgacg ctatctccgc cggcattgaa gatcaccagc ccaaggtcga
      841 ggccgtcggc gcaagcctcg agctgagccg ccgcgtccgg cccggccagg tccagcgacc
      901 gcgtccgggt ctcgacgccg taggtctggc gaagggtcgc ggccagggcc tccagttttt
      961 ccatccggcg ggccacgagg atgacgttgc agcccttggc cgccgccagc aacgccatct
     1021 ccgcgccagt gccttcggag gcgcccgaga ccaggaccca cggaccgtat ttcgatacaa
     1081 acgtcggatc gcgccggctg tcgtcggtca tgtcgtgtct ttcatcgacg gcgccgccgt
     1141 cttccaaaga aaaacccgct cacgacgcga tcgccgggag cgggaggtgg ggtggtggaa
     1201 gtcagaatgc gtagcggatc tccaccccgt aggtccgggg atcgccatag agccgggagt
     1261 cgaagccgaa gcgactggtc cggtcggttg tgttgaggag gtattcctcg tccgtcgcgt
     1321 ttttgacgta gacgcccaga tcgacattgc cgccgccgac cttcttccag tccagccgcg
     1381 catcgaccag gccatagccc ggcgcgacat tgcccggcgt cgtcaggttg tcgtcggcga
     1441 aggccacctt gctgatgtgg gcgtagctga cgttggcgac gagctcgccg gacggcaacg
     1501 catggcgata ggtcgccgag acattgaggg tgtccttcgg ggcctgggcg aacttgttgc
     1561 cggtgacgtc acccagcagc gcgttctgga agctgtcgta tttggtgtgc agatagccgt
     1621 aggaggcttg caacgtcagc ggctcgaccg gacgggccag aacctcgatc tggccgccgt
     1681 agatcgtcgc ctcggcggcg ttgaaattac ccgtcacgat caggttgtcg aacaccagcg
     1741 acgtggtgcg ctggatgtcc ttgtacttgg cgtagaaggt cgagaggttg gtccggacag
     1801 gcatgccggc caggctccaa tcggccttca gacccgcctc gtagtccgtg atgaactcgg
     1861 gcctgacgac ctcgagatcg gggtcgttga tgtttggatt gaagccgccg gcgttgtagc
     1921 ctttgcgcgt ggtcgcatag agcnnnnnnn nnnaatccac cgacaggttg taggtcaacg
     1981 catcggtctt ggctgcgatc ttctgggtgc aggtggcgat attgacgctg ggaagagcag
     2041 cgggaagatt gcacttcagg ccggggctat agccgtgctg ggccgtcttc acgtcgtctt
     2101 cggtgtagcg ggcgccagcc gtgagattca gccgatccgt cacggcatat gtgccctgag
     2161 cgtagacggc ccgggaatcc gtcgtcgtat tgccgccggt ctgctgggtg gtgttgttgt
     2221 tatgtggggt gcccaccggg gcgaacagca gatagctgtt gaagttgtcc acgtcgctgg
     2281 cggacgaata cagcccc
//
LOCUS       DAOMEO010000501         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4965_length_2295_cov_2.360714, whole genome shotgun sequence.
ACCESSION   DAOMEO010000501 DAOMEO010000000
VERSION     DAOMEO010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4965_length_2295_cov_2.360714"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLH31_11335"
     CDS             <1..378
                     /locus_tag="PLH31_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQR89826.1"
                     /translation="TAVVGIVLALLLFPLREWVASAYTADQNAIALIAPALALSCLFL
                     APDAVQVVAAQALRARGEVWIPTITHLISYGVIMGPLAWWLAIPMGLGVNGIVWSVIV
                     TSFVSAAFLLGRFRMLDIKDRSL"
     gene            complement(495..1388)
                     /locus_tag="PLH31_11340"
     CDS             complement(495..1388)
                     /locus_tag="PLH31_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HQR89827.1"
                     /translation="MSRLFSAYVIVDWSAAAKPTTGADSVWIGVLKRDVRFRLTFESF
                     NPATRQEAETKLAAILDDFKKRGERALVGFDFPLGFPRGFAKALSLPGEHPWRAVWDQ
                     LNKMVKDKADNTNNRFGVGSEINRRLTGGPFPFWGCPPKDTLTTLQPKRTREHGPDDL
                     PEFRYADEAAKGAHSIWKLYYNGSVGGQAIVGIPAARRLKDARGDAARVWPFETGFKA
                     LTEADLEGVLVVIAEVYPSLLKATPAPGEVKDLAQVRAQAEHFAKLDEAGKLAALFAA
                     PKAADEVVEAAVAEEGWILGA"
     gene            complement(1474..>2295)
                     /locus_tag="PLH31_11345"
     CDS             complement(1474..>2295)
                     /locus_tag="PLH31_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HQR89828.1"
                     /translation="GNVQHIPLVALPGLHWLSRMHLVIGVLSYVLSPLWFMALSAGVI
                     SRLLMPELKKAAFTMADLQAAAHALIDWREIQATAWAMIITFVLLFGPKILGAILVFS
                     RPNERKGFGGRRRIAAGLSVEMLLSALVAPMLMFTQTRALVEILAGKVGGWATQRRDA
                     DKVTGREAFRAMGWISVTGVTLATLFWFTPDLLTGTAPILAGLILAVPLTMLGAHKAA
                     GLRLKANGLFMTPEERRPPAIVRAALGPVCEPPARWFARDGRIAPPVEVAEQTAA"
BASE COUNT          352 a          757 c          831 g          355 t
ORIGIN      
        1 acggcggtgg tggggatcgt gctggccctg ctgctgttcc cgctgcgcga gtgggtggcc
       61 tcggcctata ccgccgacca gaacgccatc gccctgatcg cgcctgccct ggccctcagc
      121 tgcctgttcc tggcccccga cgccgtgcag gtggtggcgg cccaggccct gcgggcgcgc
      181 ggcgaggtgt ggatacccac catcacccac ctgatcagct atggcgtgat catgggcccg
      241 ctggcctggt ggctggccat tccgatgggg ctgggcgtca acggcattgt ctggtcggtg
      301 atcgtgacca gctttgtgtc ggcggcgttt ttgctggggc ggtttcggat gctggatatc
      361 aaggaccgga gcctctagct cctccccctc tgggggaggt ggcccagagg gtcggagggg
      421 ggcagctcgg cgttcgccca gatggccccc tcagtcgctc cgcgacagct cccccaaagg
      481 gggagcagct acagctaagc ccccaggatc cagccttctt cagcaaccgc cgcctcgacc
      541 acctcatcgg cagccttcgg cgccgcaaac agcgccgcca gctttcccgc ctcgtccagc
      601 ttggcgaagt gttcggcctg ggcgcgcacc tgggccaggt ccttgacctc gccgggggcg
      661 ggcgtggcct tcagcaacga gggatagacc tcggcgatca ccaccagcac gccctccaga
      721 tcagcctcgg tcagggcctt gaaacccgtc tcgaacggcc agacccgggc ggcgtcgccg
      781 cgggcgtcct tcagacggcg ggcagccggg atgccgacga tcgcctgacc gcccaccgag
      841 ccgttgtaat agagcttcca gatcgagtgc gcgcccttgg cggcctcgtc ggcgtagcgg
      901 aactccggca ggtcatcggg gccatgctcg cgcgtgcgct tgggctgcag ggtggtcagc
      961 gtgtccttgg gcgggcagcc ccagaacggg aaggggccgc cggtcaggcg gcggttgatc
     1021 tcggagccga cgccgaaacg gttattggtg ttgtcggcct tgtccttgac catcttgttc
     1081 aactggtccc agaccgcgcg ccacgggtgc tcgcccggca ggctcagggc cttggcgaag
     1141 ccgcgcggga agcccagcgg gaagtcaaag cccaccaggg cccgctcgcc gcgcttcttg
     1201 aagtcgtcga gaatggccgc cagcttggtc tcggcttcct gacgggtggc cggattgaag
     1261 ctctcgaagg tcagccggaa ccgcacgtcg cgcttgagca cgccgatcca gacggagtcc
     1321 gcgccggtgg tcggcttggc ggccgcgctc cagtcgacga tcacataggc gctgaaaagg
     1381 cgcgacacgg caggctccgt cggaaacgaa cgaagccgcg cttctactcc gcccggatcg
     1441 caaggaccag cgggcgttcg cgcctaaagc gtattaggcg gcggtttgct cggcgacctc
     1501 gaccggcggg gcgatgcggc cgtcgcgggc gaaccagcgg gcggggggct cgcagaccgg
     1561 gcccagggcc gcgcggacga tggccggcgg gcggcgctct tccggggtca tgaacaggcc
     1621 attggccttc agccgcaggc cggcggcctt gtgggcgccc agcatggtca gcggcacggc
     1681 caggatcagg ccggccagga tcggcgcggt gccggtcagc aggtcgggcg tgaaccagaa
     1741 cagggtggcc agtgtgacgc cggtgacgct gatccagccc atggcgcgga aggcttcgcg
     1801 gccggttacc ttgtctgcgt cgcggcgctg ggtggcccag ccgccgacct tgccggccag
     1861 gatctcgacc agagcgcggg tctgggtgaa catcagcatg ggggcgacga gcgccgagag
     1921 cagcatctcg acgctgaggc cggcggcgat gcggcggcga ccgccaaagc ccttgcgctc
     1981 gttggggcgc gagaagacca ggatggcgcc caggatcttg gggccaaaca gcagcacgaa
     2041 ggtgatgatc atcgcccagg cggtggcctg gatttcgcgc cagtcgatca gggcgtgagc
     2101 ggcggcctgc aggtcggcca tggtgaaggc ggccttcttc agttccggca tcagcaggcg
     2161 agagatcacg ccggccgaca gggccatgaa ccacaggggc gacagcacat agctcagcac
     2221 gccgatgacg aggtgcatgc ggctcagcca gtgcaggccg ggcagggcga ccagcggaat
     2281 gtgctggaca ttgcc
//
LOCUS       DAOMEO010000502         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4966_length_2295_cov_2.337054, whole genome shotgun sequence.
ACCESSION   DAOMEO010000502 DAOMEO010000000
VERSION     DAOMEO010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_2295_cov_2.337054"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLH31_11350"
     CDS             <1..469
                     /locus_tag="PLH31_11350"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HQR89829.1"
                     /translation="LELQEAAMITQGRTMVVAADDARARLFEEPRRGGPLTERSEWLA
                     DLAPRDFPDPGSGGIHDRMGYAIHGAATVKAADKSARDFLVRLVARLDSIVNEQDFDH
                     LVIFAPPRALGMLRVALPRSLRQRLALDQDNDRVDAGPEALREALSALRLNAS"
     gene            546..2231
                     /locus_tag="PLH31_11355"
     CDS             546..2231
                     /locus_tag="PLH31_11355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR89830.1"
                     /translation="MTPILSFHGAAGCVTGSCARLQTQQAMILIDCGMFQGSKSLKAL
                     NYGEFPFDPRQVDAVLLTHAHVDHCGLLPKLMLAGFEGPIYATAATRDLCAVMLADAG
                     GIQEAEVAALNRRNQRRGLAPVEPIYTATDAARVMKQFRKVKLGERVEVAPEISAVYW
                     EAGHILGSASIEVAIGPAGAEKRLLFSGDLGSGGSDFVADPQGPTGVDHLIMESTYGD
                     RERAVSSGAMRRVALAEELKAAHAAGGPLLIPVFALQRSQELLVDLLQLMDEGGAPRG
                     DIFLDSPLAIEASEVFLERGWNPALQRNPFEPVRPSANLRFLAKPWDSDGLERLSGWH
                     VILAASGMCDAGRVRKHLKRLLWRKEATVLLSGFQAAGTLGRLLADGAPRVSIQGDEV
                     RVRARVRKIEAYSAHADALGLTRWARDRAPVAGSILLAHGEPAAAQGLRDRLVAAGFD
                     ADAILIPDLDEAFDLTEGRVTPRQPSPARLPPHTAARLDWHNHRVAFQMALNAALQGA
                     EDDKAREALLRRLREVVEGQPGGAPARPPGARGSPHSPELDSGQRSSSRDEAF"
BASE COUNT          360 a          766 c          778 g          391 t
ORIGIN      
        1 cttggagctt caggaggcag cgatgattac gcaaggaaga accatggtcg tggcggccga
       61 tgatgcccgg gcccggcttt tcgaggaacc gcggcgcggc gggcccctga cggaacgttc
      121 cgagtggctg gctgatctgg ccccgcgcga ctttcccgat cccggttcgg gagggatcca
      181 tgatcggatg ggctatgcca tccacggcgc ggccaccgtc aaggcggccg acaagagcgc
      241 ccgcgacttc ctggtccggc tcgtcgcgcg gctcgacagc atcgtcaatg aacaggactt
      301 tgatcatctc gtcattttcg cgccgcctcg cgccctgggc atgttgcgcg tggcgttgcc
      361 aagatccctg cgccaacgtc tggcgctgga tcaggataac gaccgtgtcg acgccgggcc
      421 cgaagccctg cgcgaagccc tgagcgcgct gcggctgaac gcgtcgtgag ccgacgaacc
      481 cctgctcctc ccgccttgca cggtggcgtc gcgcccggtc gtctcctgcc gtcgaaggcc
      541 acagcatgac cccgatcctg tcctttcatg gcgccgctgg ctgtgtgacc ggatcctgcg
      601 cgcggttgca gacgcaacag gccatgatcc tgatcgactg cggcatgttc cagggatcaa
      661 agagcctcaa ggccctgaac tatggcgagt tcccgttcga tccacgccag gtcgacgcgg
      721 ttctgctcac ccacgcccat gtcgaccact gcggtctgct tcccaagctg atgctggcgg
      781 gcttcgaggg gccgatctat gcaacggcgg cgacacggga tctctgcgcg gtgatgctcg
      841 cagacgcagg cggcattcag gaggcggagg ttgcggcgct caaccgccgc aaccagagac
      901 ggggcctggc ccctgtggag ccaatctata ccgcgactga cgcagcgcgg gtcatgaagc
      961 agttccggaa agtcaaactg ggcgaacggg tagaggtcgc tcccgagatt tcggcggtct
     1021 attgggaggc gggacatatc ctcggctcgg cgtccatcga ggtggccata ggtccagcgg
     1081 gcgcggaaaa gcgcctgctg ttctcaggcg atctgggttc cggcggtagc gactttgtcg
     1141 ccgacccgca gggcccaacg ggcgtcgacc atctgatcat ggaatccacc tatggtgatc
     1201 gcgagcgcgc ggtctccagc ggcgccatgc gccgcgtggc cctggcggag gaactcaagg
     1261 ccgcccacgc cgccggcggg ccgctgctga tccccgtctt cgcgcttcag cgcagccagg
     1321 agcttctggt cgatctgcta cagttgatgg acgagggggg cgcgcctcgc ggcgacattt
     1381 ttctggactc gcccctggcc attgaggcgt cggaggtctt cctggagcgg ggctggaacc
     1441 ccgcgcttca gcgcaatccg ttcgaaccgg tccggccgtc cgcaaacctg cgctttctgg
     1501 ccaagccctg ggatagcgac gggctggagc ggctgtctgg ctggcatgtg atcctggcgg
     1561 ccagcggcat gtgcgacgcc ggcagggtgc gcaagcacct caagcggctt ctgtggcgca
     1621 aggaggcgac ggtgctgctt agcggattcc aggccgccgg cacgctgggc cggttgctcg
     1681 cggacggtgc gccgcgggtc agcatacagg gtgacgaggt gcgggttcgc gcccgggtgc
     1741 gcaagatcga ggcctattct gcccatgccg acgcgttggg tctaacccgt tgggcgcggg
     1801 atcgtgcgcc ggtcgcgggt tcgatcctgc ttgctcacgg ggaaccggcc gccgcccagg
     1861 gactgcggga ccggctggtc gccgccggct ttgacgccga cgccatcctg atcccggacc
     1921 tggacgaggc cttcgacctg accgaggggc gcgtcacgcc aaggcagcca tcgcctgccc
     1981 gactgccgcc acacacggca gcccggctcg actggcacaa tcaccgggtc gctttccaga
     2041 tggccctcaa cgcggccctg cagggtgctg aggacgacaa agcccgtgag gcgctgctgc
     2101 gccgcctgcg tgaggtggtc gaaggtcagc ctggcggcgc ccctgctcgg ccacccggag
     2161 cgcgaggctc gccccattcg cccgaacttg actcaggtca acgctcgtca tcgcgagatg
     2221 aagcgtttta gggcctcaac accccccctt tgcaaggcag gacaatgtca gctcgcaaga
     2281 tcaagacgct cgccc
//
LOCUS       DAOMEO010000503         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4970_length_2294_cov_2.675748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000503 DAOMEO010000000
VERSION     DAOMEO010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4970_length_2294_cov_2.675748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLH31_11360"
     CDS             <1..253
                     /locus_tag="PLH31_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR89831.1"
                     /translation="KAHYDCIKAFSETDFTEDLKKITVPTLVMHGEDDQIVPIADSAL
                     LAVKLLKKGELKTYPGLPHGMATTHADVINADLLAFFKA"
     gene            complement(318..653)
                     /gene="mazF"
                     /locus_tag="PLH31_11365"
     CDS             complement(318..653)
                     /gene="mazF"
                     /locus_tag="PLH31_11365"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HQR89832.1"
                     /translation="MSGRYVPEAGDIVWLQFDPQAGHEQAGHRPALVLSPSSYNGKRG
                     MMICCPMTSRVKGYVFEVAVPGAEPSAVLADQVKSLDWVARRATRKGVAPPEVLAEVR
                     SKLKALLSF"
     gene            complement(650..892)
                     /locus_tag="PLH31_11370"
     CDS             complement(650..892)
                     /locus_tag="PLH31_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQR89833.1"
                     /translation="MQVLIKKWGNSASVRIPATVMAAASISVDQAVDVREENGRIIIE
                     PIRAPGYDLNDLLARMTPETFPDDADFGAPVGEEVW"
     gene            complement(960..2267)
                     /locus_tag="PLH31_11375"
     CDS             complement(960..2267)
                     /locus_tag="PLH31_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HQR89834.1"
                     /translation="MTTVAEVRLWGSRIGAVSLEDGAETAAFAYEPAFIGSGVQPAPI
                     MMPLGPGVFSFPDLPRRSFHGLPGMLADALPDKYGNVLIDAWLATQGRTPQSFNAVER
                     LCYTGRRGMGALEFSPMAGPRRRVSSKIDIDALVTLASQVLTHRHDLRASFADAGKAD
                     ALRDILSVGTSAGGARAKAVVAWNPTTNEVRSGQVEAGEGFGYWLLKFDGVSGNKDKE
                     LADPKGYGAVEHAYGRMAAAAGVTVAESRLLEEGGRRHFMTKRFDRLEGGGKLHMQSL
                     AAIAHLDFNDPVANSYEQALFTMRRMGLPMTQLEEQFRRMVFNVLARNQDDHVKNIAF
                     LMDRSGAWRLSPAFDITWSYNPAGEWTSRHQMSINGKRDGFEFADLEACARTASINRG
                     HVSRIFDEVRDAVSRWPSFADAAGVDERWRDEIAATLRLELAR"
BASE COUNT          427 a          782 c          707 g          378 t
ORIGIN      
        1 caaggcccat tacgactgca tcaaggcctt ctcggaaaca gacttcaccg aagacctgaa
       61 gaagatcacc gtccccaccc tggtcatgca cggcgaagac gaccagatcg tccccatcgc
      121 ggactctgcg ctgctggcgg tgaagctgct gaagaagggc gaactgaaga cctatccggg
      181 cctgccgcac ggcatggcga cgacgcatgc ggatgtgatc aacgccgacc tgctggcgtt
      241 cttcaaggcg taggggtcga aactgggaac ttgggagcgc tcgccgaaag acgggcgctt
      301 cttcgctagg atgcccatca gaagctcagc agcgccttca gtttggaccg cacttccgcc
      361 agaacctccg gcggagcgac accttttcgc gtggcgcgac gagcgaccca gtccaggctc
      421 ttgacctggt cggccagaac cgcgctgggc tctgcacctg ggacagccac ttcaaacaca
      481 tagcctttga cccggctcgt catcggacag cagatcatca tgccgcgctt gccattgtag
      541 ctggatggcg acagcaccag ggccggtcga tgaccagcct gctcatgccc ggcctgcgga
      601 tcaaactgaa gccagacgat atcgccagcc tctggcacat agcgcccact caccagactt
      661 cttcgcccac gggagcgccg aagtcggcat cgtcagggaa agtctcaggg gtcatccgag
      721 ccagaagatc attcaggtcg taacccgggg cccggatcgg ctcgatgatg atccggccgt
      781 tctcttcgcg cacgtcgaca gcctgatcga cgctgatcga ggcggccgcc atcaccgtcg
      841 ccgggatgcg gactgaagcg ctgttgcccc acttcttaat caaaacctgc atgagcgatc
      901 ctcggataca ggttacaaca tcgcatacgt atcgacaaat gtcgatacgt cgaacagatc
      961 tacctcgcca gctccaaccg caacgttgca gcgatctcat cgcgccagcg ctcgtccacg
     1021 ccagccgcgt cggcgaagga cggccatcgc gagacggcgt ctcgcacctc gtcaaagatc
     1081 cggctgacat ggccgcgatt gatcgaggcg gtcctggcgc aagcctcaag gtcggcaaac
     1141 tcgaacccgt cccgcttgcc attgatcgac atctgatgcc gcgacgtcca ctccccggcg
     1201 gggttgtagc tccaggtgat gtcgaaggcc ggcgacagtc gccaggcgcc cgaacggtcc
     1261 atcaggaagg cgatgttctt gacgtgatcg tcctggttcc gcgccagcac gttgaagacc
     1321 atgcgccgga actgctcttc caactgcgtc atcggcaagc ccatccgccg catggtgaac
     1381 agggcctgct cataggaatt ggcgaccgga tcgttgaagt ccagatgggc gatggccgcc
     1441 agtgactgca tgtgcagctt gccgccgccc tccagccggt cgaaccgctt ggtcatgaag
     1501 tggcgacgcc cgccttcttc gagcaggcgg ctctcggcca cggtgacgcc ggccgccgcc
     1561 gccatgcgcc cataggcgtg ctcgaccgcg ccatagccct tggggtcggc cagttccttg
     1621 tccttgttgc ccgagactcc gtcgaacttc agcagccagt agccaaagcc ctcgccggcc
     1681 tcgacctggc cggagcgcac ctcgttggtg gtcgggttcc aggcgaccac cgccttggcc
     1741 cgcgccccgc cggccgaggt gccgacgctg agaatgtcgc gcagggcgtc ggccttgccc
     1801 gcgtcggcga acgaggcgcg caggtcgtgg cggtgggtca gaacctgcga ggccagggtc
     1861 accagggcgt cgatatcgat cttgctggag acccggcggc gcggcccggc catgggcgaa
     1921 aactccagcg cgcccatgcc ccgtcgcccg gtgtagcaaa gccgctcgac ggcgttgaag
     1981 ctctgaggcg tgcgcccctg ggtggccagc caggcgtcga tcagcacatt gccgtacttg
     2041 tcgggcaggg cgtcggccag catcccgggc aagccatgga agctgcgacg cggcaggtcg
     2101 ggaaagctga acacccccgg ccccagcggc atcatgatcg gagcgggctg aacgccgctg
     2161 ccgatgaagg ccggctcata ggcaaaggcc gccgtctccg cgccgtcctc cagcgacacc
     2221 gcgccgatac ggctgcccca cagccggacc tcggcgacgg tggtcatttt tcatcgcccc
     2281 agacccaggc ggcg
//
LOCUS       DAOMEO010000504         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4975_length_2292_cov_2.586947, whole genome shotgun sequence.
ACCESSION   DAOMEO010000504 DAOMEO010000000
VERSION     DAOMEO010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4975_length_2292_cov_2.586947"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1835)
                     /locus_tag="PLH31_11380"
     CDS             complement(225..1835)
                     /locus_tag="PLH31_11380"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HQR89835.1"
                     /translation="MSMRAFAHLLDRLSLTSSRNAKLTLIEDFLRGQPDPDRGFALAA
                     LTGALAFSAAKPAFIRKAIEARMDAQLFAWSYDYVGDLAETVALAWPSRPGANREPEL
                     SEVIEALRGASRSEVQRLIEDWLDALDADGRWALLKLMTGGLRVGVSARLAKQACANL
                     GKVEIGEIEEVWHALEPPYGDLFAWLEGRSERPSPDAPGRFRPVMLAQAIDEAVDFAK
                     LDPADYVAEWKWDGIRVQAVSERGERRLYTRTGDDISATFPDVLAALDFDGALDGELV
                     VIRDGALAAFGDLQQRLNRKTVDTRQLTAFPAGIRAYDLLLDGETDVRSLPFIERRAR
                     LEAFVARIANARIDLSPLQPFETWEELTALRAEPPEGDARLSEGLMLKRRDSVYEPGR
                     PKGPWFKWKRDPRLIDAVLMYAQRGHGRRSSFYSDYTFGAWREDELGARTLTPVGKAY
                     FGFTDEELKQIDKFVRDNTVERFGPVRSVRADLDFGLVFEVAFEGLQRSTRHKSGVAM
                     RFPRISRIRWDKPSRDADILETLERMLD"
     gene            complement(1875..>2292)
                     /locus_tag="PLH31_11385"
     CDS             complement(1875..>2292)
                     /locus_tag="PLH31_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89836.1"
                     /translation="GLEPAVNLILLSGAAAWFLVSLETRWKRGRVQKALHELRSFAHV
                     IDMHQLTKDPTLVLGPRTPSSPVREMNRFQLARYLDYCAEMLALTAKLAALYAGETDD
                     PVIVAAVNDIETLTSDLGRKIWQKIMILGQLDETRT"
BASE COUNT          411 a          776 c          747 g          358 t
ORIGIN      
        1 acgctcgcca gcaaccaggc aatgatcagg tcgtcacgcc cgatcaggtg caacgcccag
       61 ctgagcagga ccgtcagacc cagccgcgag aagacctgcc ggtatcgctc gtcggcgttg
      121 gcgggcagat gaggcagcaa cgaatccagg aactgaggca tgcgcggtct ccctaccgtc
      181 gatgcctaag ccgacatggc aaacgagatg tgaacgctca agcttcagtc gagcattcgc
      241 tccagcgttt cgagtatgtc cgcgtcgcgc gatggtttgt cccagcgaat tcggctgatg
      301 cgcgggaagc gcatggcgac gcccgacttg tggcgggtcg agcgctgcaa cccctcgaag
      361 gccacctcga acaccaggcc aaagtccaga tcggcccgca ccgagcgcac cggaccgaac
      421 cgttcgaccg tgttgtcgcg cacgaacttg tcgatctgct tcagctcctc gtcggtgaag
      481 ccgaaatagg ccttgcccac cggggtcagg gtccgggcgc ccagctcatc ctcccgccag
      541 gcgccgaacg tgtagtcgga atagaagctg gagcgccggc catgcccccg ctgggcgtac
      601 atcagcaccg cgtcgatcag ccggggatcg cgcttccact tgaaccacgg ccccttggga
      661 cgccctggct cgtagacact gtcgcgacgc ttcagcatca gaccttccga cagccgcgca
      721 tcgccctcgg gcggctcggc ccgcagggcg gtcaattcct cccaggtctc aaacggctgt
      781 agcggcgaca ggtcgatacg ggcgttggcg atccgggcca cgaaggcttc cagacgggct
      841 cggcgctcga tgaaggggag ggaccgcaca tcggtctcgc catccagcag cagatcatag
      901 gcgcggatac cggccgggaa cgcagtcaac tgccttgtgt cgacggtctt gcggttcaac
      961 cgctgctgca ggtcgccgaa ggctgccagg gctccgtcgc ggatcaccac caactcgccg
     1021 tccagggcgc cgtcgaaatc cagcgccgcc agcacgtcgg gaaaggtcgc cgaaatgtcg
     1081 tcgccggtgc gggtgtagag ccgccgctcg ccccgctccg aaacggcctg cacgcggatg
     1141 ccgtcccatt tccattccgc cacgtagtcg gccggatcga gctttgcgaa atccacggct
     1201 tcatcgatcg cctgggccag catcaccggc ctgaaacgcc ccggcgcatc cggcgacggg
     1261 cgttcggatc ggccctccag ccaggcaaag agatcgccgt agggcggctc cagggcgtgc
     1321 cagacctcct cgatctcgcc aatctcgacc ttgccgaggt tggcgcaggc ctgcttggcc
     1381 agccgcgccg agacgccgac ccgtaggccg ccggtcatca gtttcagcag cgcccagcgg
     1441 ccatcggcgt ccagagcgtc cagccagtcc tcgatcagcc gctggacctc cgagcgcgag
     1501 gccccgcgca gggcctcgat gacttccgac agttccggct cgcgattggc gcccggccgc
     1561 gaaggccagg ccagggcgac ggtctcggcc aggtcaccga catagtcgta ggaccaggcg
     1621 aacagctggg cgtccatccg cgcctcgatc gccttgcgga tgaaggccgg cttggcggcg
     1681 gaaaaggcga gcgcgccggt cagggccgcc agggcaaagc cccggtcggg gtcaggctga
     1741 ccgcgcagaa agtcctcgat cagggtcagc ttggcgttgc gcgagctggt cagggacagc
     1801 cggtccagca gatgggcgaa ggcgcgcatg gacatcgaac gtctggtctc ggctcaggtt
     1861 cagaccccgg gggttcaggt gcgggtttcg tccagctgac ccaggatcat gatcttctgc
     1921 cagatcttgc gacccaggtc agaagtcagg gtctcgatgt cattgacggc ggcgacgata
     1981 acgggatcgt cagtctcgcc cgcatacaag gccgccagct tggcggtcag ggccagcatt
     2041 tccgcgcagt agtccagata gcgagccagc tggaaacgat tcatttcgcg caccggcgag
     2101 gagggcgtgc gcggccccag caccagggtc gggtccttgg tgagctggtg catgtcgatc
     2161 acgtgggcga aggatcgcaa ctcgtgcagc gccttttgca cccggccgcg cttccagcgg
     2221 gtctccagcg agaccaggaa ccaggcggcc gctcccgaca gcaggatcag attgaccgcc
     2281 ggctcaagtc cc
//
LOCUS       DAOMEO010000505         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4979_length_2291_cov_2.520572, whole genome shotgun sequence.
ACCESSION   DAOMEO010000505 DAOMEO010000000
VERSION     DAOMEO010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_2291_cov_2.520572"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLH31_11390"
     CDS             complement(<1..187)
                     /locus_tag="PLH31_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89837.1"
                     /translation="MAYDAKQIAKAAGGLTLAGAPEGFDALVMGDIARARGGLTAFVA
                     RDTARANAFADALKFFAP"
     gene            complement(197..490)
                     /locus_tag="PLH31_11395"
     CDS             complement(197..490)
                     /locus_tag="PLH31_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQR89838.1"
                     /translation="MTIDHDSRLRRLRFRAWHRGFREADLILGPFADNHGQNLTPEQL
                     DTLEALLENPDREIYAWIVGQEATPPEFETDVLAMIRRFRFEAHAARPVGDGA"
     gene            672..>1954
                     /locus_tag="PLH31_11400"
     CDS             672..>1954
                     /locus_tag="PLH31_11400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR89839.1"
                     /translation="MTEFSELGLSPTTLQAVADTGYTTATPIQAQAIPVALAGQDVLG
                     IAQTGTGKTAAFTLPLIDKLMTGRAKARMPRALVIAPTRELADQVASSFEKYAKGTKL
                     SWALLIGGVSFGDQEKKLDRGVDVLIATPGRLLDHFERGKLLMTGVQFLVVDEADRML
                     DMGFIPDIERIFKMTPPKKQTLFFSATMPPEITRLTKQFLKDPVRIEAARPATTNENI
                     TQLLVKVPSSDPKAKRLALRALIEKAQIETGIVFCNRKTEVDVVAKSLRSHGFDAAAI
                     HGDLDQSQRMKTLAAFRDGSLKILVASDVAARGLDIPAVSHVFNYDVPHHADDYVHRI
                     GRTGRAGRSGITYMLVTPGDDKGFDKVIKLIGSTPVEEKLDLDYSQAVTVHRDGDKKR
                     GSRGAPSRDRGGEREPGERSARGGRGARTREAEAAP"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1965..>2291)
                     /locus_tag="PLH31_11405"
     CDS             complement(<1965..>2291)
                     /locus_tag="PLH31_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89840.1"
                     /translation="AANRLNAAHGRGRTDFALWTRAVMSAFARGDNVGFDSCGNFRAL
                     GTGTTVRAIAPAITAALARGRRFGFGLSFDFDLNDGFNHFGFDDAFRTLGARTAFTRF
                     ALAAATG"
BASE COUNT          404 a          792 c          729 g          356 t
ORIGIN      
        1 cgggggcgaa gaacttcagg gcgtcggcga aggcgttggc ccgggccgtg tcgcgggcca
       61 cgaaggcggt caggccgccg cgagcgcggg cgatgtcgcc catcaccagg gcgtcgaagc
      121 cctcgggcgc gccggcaagg gtcaggccgc ccgcggcctt ggcgatttgc ttggcgtcgt
      181 aggccatcag ggtctcttaa gcgccgtcac caacggggcg agcggcatgg gcctcgaagc
      241 ggaacctgcg gatcatcgcc agcacatccg tctcgaattc aggaggcgtg gcttcctgcc
      301 ccacgatcca ggcgtagatc tcgcgatccg gattttccag aagcgcctcg agcgtatcca
      361 gctgttctgg cgtcaggttc tggccatggt tatccgcgaa cggccccaga atgaggtccg
      421 cttcccggaa accgcgatgc caggcgcgga agcgaagccg ccgaaggcgg gagtcgtgat
      481 caatggtcat gtgcctggcg gatatagagc ttactcaggc ttggcgccag tcacgctggt
      541 cgtccccagt cttcgtcgag attgactttc cgggtgttca gcctatttat ccccagcttc
      601 ggagcaggtc aggcgaatcc gcgccgcccg ttcgcccaaa cacgggcggc tctgaattca
      661 gccagattga tatgacagaa ttttccgaac tcgggctgtc gcccacgacc ctgcaagcgg
      721 tcgcagacac cggctacaca acggccaccc ccattcaggc gcaagccatt ccggtggccc
      781 tggccggtca ggacgtcctg ggcatcgccc agaccggcac cggcaagacc gccgccttca
      841 ccctgccgct gatcgacaag ttgatgacgg gccgcgccaa ggcgcggatg ccgcgcgccc
      901 tggtcatcgc cccgacccgc gaactggccg accaggtcgc ctccagtttc gaaaagtacg
      961 ccaagggcac caagctctcg tgggcgcttc tgatcggcgg cgtgtcgttc ggcgatcagg
     1021 agaagaagct ggatcgcggc gtcgacgtgc tgatcgccac ccccggccgc ctgctggacc
     1081 atttcgagcg cggcaagctg ttgatgaccg gcgtgcagtt cctggtcgtc gacgaagccg
     1141 accggatgct ggacatgggc ttcatcccgg acatcgagcg catcttcaag atgacgccgc
     1201 ccaagaagca gaccctgttc ttctcggcga ccatgccgcc ggaaatcacc cgactgacca
     1261 agcagttcct gaaggatccc gtgcggatcg aggcggcgcg tcccgccacg accaacgaga
     1321 acatcaccca actactggtc aaggtcccct cctccgatcc caaggccaag cgcctggcgc
     1381 tgcgcgcgct gatcgagaag gcccagatcg aaaccggcat cgtgttctgc aaccgcaaga
     1441 ccgaggtcga cgtggtcgcc aagtcgctgc gctcgcatgg cttcgacgcc gccgcgatcc
     1501 acggcgacct ggaccagagc cagcgcatga agaccctggc ggccttccgc gacggttcgc
     1561 tgaagatcct ggtggcctct gacgtcgccg cgcgtggtct ggatatcccg gccgtcagtc
     1621 acgtcttcaa ctacgacgtc ccgcaccacg ccgacgacta tgttcaccgc atcggtcgca
     1681 ccggccgcgc cggtcgttcg ggcatcacct acatgctggt gacgccgggc gacgacaagg
     1741 gcttcgacaa ggtcatcaag ctgatcggct cgaccccggt cgaagaaaag ctcgacctcg
     1801 actactcgca agccgtcacc gtccatcgcg atggcgacaa gaagcgtggc agccgtggcg
     1861 cgcccagccg tgatcgcggc ggcgaacgcg agccgggcga acgctcagcc cgcggcggtc
     1921 gtggcgcccg cacgcgcgag gccgaagcgg ctccnnnnnn nnnngcccgt agccgcggcg
     1981 agcgcaaacc tcgtgaacgc ggtccgcgcg ccgagcgtcc tgaacgcatc gtcgaacccg
     2041 aaatggttga agccgtcgtt caggtcgaag tcgaagctga gcccgaaacc gaagcgcctc
     2101 ccccgcgcga gcgcggccgt gatcgcgggc gcaatcgctc ggaccgtggt ccccgtcccg
     2161 agcgcgcgga agttgccgca gctgtcgaag ccgacgttgt cgccccgcgc gaacgcactc
     2221 atgaccgcgc gcgtccagag cgcgaagtcc gtccggcctc gcccgtgcgc ggcgttcagc
     2281 cggttcgcgg c
//
LOCUS       DAOMEO010000506         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4983_length_2290_cov_2.592394, whole genome shotgun sequence.
ACCESSION   DAOMEO010000506 DAOMEO010000000
VERSION     DAOMEO010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_2290_cov_2.592394"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLH31_11410"
     CDS             complement(<1..1126)
                     /locus_tag="PLH31_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine transferase"
                     /protein_id="HQR89841.1"
                     /translation="MVESDDLAVLQTITTQGLSLGDLINAAGRYTDAGQSAMAEQVYK
                     IWIQFNPDHPQLFIALFNCATLQSGIGGTEAAMANLERAIALNPDFLPAYINLGGLQE
                     RAGALEVAITTWNAAATRPLAITGSNAHYVFSAYKQMARALLEAQQAGRAETALWRCA
                     DLDPTQLDAVGQLVAARLGQCKWPPLEPWERVDRRALLKGINPLSVAAYSDDPLLQLG
                     AAAAFTERDGSCAPEAIIAERRDAPIDLTGRRLRVGYVSSDLRDHAVGYLMAELFELH
                     DREKVEVFAYYCGPPATDALNARTRAAVEHYTDIRGMTDDQAARRIADDQIDILVDVN
                     GHTRDSRTPVFARRPAPILVNWLGYPGTMGSPFHHYIIGDP"
     gene            complement(1275..>2290)
                     /locus_tag="PLH31_11415"
     CDS             complement(1275..>2290)
                     /locus_tag="PLH31_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ice nucleation protein"
                     /protein_id="HQR89842.1"
                     /translation="IGSDFTTTQIAALSAAQVGAFNPSVLPGMTETQVATLSTTQVKG
                     LTASQITALTTTQVGALADTQLAALSILQLAALNATNLSALDETQMAGISATQIKGLT
                     NTQLKGLSTTDIAELSATQVSALTTGQITAFATTALQALSTTQVAALSTAQLGALTAT
                     SFSALADTQVAALSTTQIKGLTTAQLGGLTTTDSGELLDTQVAALTTSQLAALSPTNI
                     SALVDTQVAALSTTQVKGLTVNQIKGLSTTDIGELFDTQIAALTTSQIAALSATVIGG
                     LTETQVGSLQGTQIASLTVSQLDNLTTTSISALTATQAAALTTTQVQSLSDPQIVAYL
                     GVS"
BASE COUNT          349 a          707 c          835 g          399 t
ORIGIN      
        1 atggatcgcc gatgatgtag tgatggaacg ggctgcccat ggtgcccgga tagcccagcc
       61 agttgaccag gatcggggct ggacgacggg cgaagaccgg cgtgcgtgag tcgcgcgtgt
      121 ggccgttcac atcgaccaga atgtcgatct ggtcatcggc gatcctgcga gcagcctggt
      181 cgtcggtcat gccgcgaatg tcggtgtagt gctcgacggc cgcccgggtg cgggcgttca
      241 gggcgtcggt cgccggcggg ccgcaataat acgcaaagac ctcgaccttc tcacggtcgt
      301 gcagctcgaa cagctcggcc atcagatagc cgacggcgtg gtcgcgcagg tcggacgaca
      361 catagcccac ccgcagccgg cggccggtca ggtcaatggg cgcatcgcgg cgctcggcga
      421 tgatggcctc gggcgcacaa ctgccgtcgc gctcggtgaa ggcggcggcg gcgcccagtt
      481 gcagcagcgg gtcgtctgaa taggcggcca ccgacagcgg gttgatcccc ttgagcagag
      541 cgcggcggtc gacgcgctcc cacggctcca gcggcggcca tttgcattgc cccagccggg
      601 ccgcgaccag ctggcccacg gcatccagct gggtggggtc aagatcggcg cagcgccaaa
      661 gggcagtctc ggcgcggccg gcctgctgag cctccagcag ggcgcgggcc atctgcttgt
      721 aggctgaaaa gacgtagtgg gcatttgagc cggtgatggc caggggacgc gtggccgcag
      781 cgttccaggt ggtgatcgcc acctccaggg cccctgcccg ctcctgcaga ccgcccagat
      841 tgatataggc cggcaggaaa tccggattga gggcgatcgc ccgctccaga ttggccatcg
      901 ccgcctcggt cccgccgatc cccgactgca gggtcgcgca gttgaacagg gcgatgaaca
      961 gctgcgggtg atccggattg aactggatcc agatcttgta gacctgctcg gccatggccg
     1021 actggccggc gtcggtgtag cgcccggcgg cattgatcag atcgccgagc gacagcccct
     1081 gcgtggtgat cgtctgaagt acggcgaggt cgtcggattc aaccatgatc atctcagcgc
     1141 gcggatcggg ccggagcgag cccgactcgt ctcgtcagat gttcgcacgt cggggcagcg
     1201 tcaccaatca ccgcccggcc cggctatcct cctctggccg ccgacgtctt gcgacgccgg
     1261 cgaaccggag ggtcttagct cacgcccagg taggcgacga tctgcggatc cgagaggctc
     1321 tggacctggg tcgtcgtcag ggcggcggcc tgggtggcgg tcagggccga gatgctggtc
     1381 gtggtcaggt tgtccagctg gctgaccgtc aagctggcga tctgggtgcc ttgcagcgag
     1441 ccaacctggg tctcggtcag accgccgatc accgtggccg acagagccgc gatctgactg
     1501 gtggtcagcg ccgcgatctg ggtgtcgaac agctcgccga tatcggtggt cgacagaccc
     1561 ttgatctggt tgaccgtcag ccccttgacc tgggttgtgc tcagcgccgc cacctgggtg
     1621 tcgaccagag ccgagatgtt ggtcgggctc agcgcagcca gctggctggt ggtgagggcg
     1681 gcgacctggg tgtcgagcag ttcgccgcta tcggtcgtcg tcaggccgcc cagctgcgca
     1741 gtcgttagac ccttgatctg ggtggtgctg agcgccgcca cctgggtgtc ggccagggcc
     1801 gagaagctgg tggccgtcag ggcgcccagt tgcgccgtgc tgagcgcggc gacctgggtg
     1861 gtcgacaacg cctgcagggc ggtagtggcg aaggcggtga tctggccggt cgtcagggcg
     1921 ctgacctggg tcgccgacag ctcggcgatg tccgtggtcg acagaccctt cagctgggta
     1981 ttggtcaggc ccttgatctg ggtcgccgag atgcccgcca tctgggtttc gtccagcgcc
     2041 gacaggttgg tcgcgttcag ggcggccagt tgcaggatgc tgagcgcggc caactgggtg
     2101 tcggccaggg cgccgacctg agtggtggtc agggccgtga tttgcgaggc cgtcaggccc
     2161 ttgacctggg tcgtgctgag agtcgcgacc tgggtctcgg tcataccggg cagaacgctc
     2221 ggattgaatg cccccacctg ggccgccgac agagcggcga tctgggtggt ggtgaagtca
     2281 gacccgatat
//
LOCUS       DAOMEO010000507         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4984_length_2290_cov_2.511857, whole genome shotgun sequence.
ACCESSION   DAOMEO010000507 DAOMEO010000000
VERSION     DAOMEO010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_2290_cov_2.511857"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLH31_11420"
     CDS             <1..1427
                     /locus_tag="PLH31_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR89843.1"
                     /translation="FTTEEYMQRPANANVSPENFERIVALVRDLVPGGEPRVFEYESV
                     HKDGSVIWIESTYSRLAGEEGELLVTSRVTTERKKLEIELTNALEAAQTAAAAKSDFL
                     ANMTHELRTPLNAIIGFAGVLRGSSTLSERDARHVGLIQDASNTLLTVVNDVLDFSRL
                     EAGGVELDPQPFDPRVMVASCAGLIDGQARAKGLVLQVSAPDDLRVMQADGPRLTQVL
                     LNFLSNAVKFTNSGGVTVNVRQVLDGDQGLLRVEVTDTGIGIAEHQLDGVFDRFSQAD
                     AAVSRRFGGTGLGLAISRRIIEQMDGEIGVDSREGEGSRFWFEIRAPLCELAPETLLE
                     EPTLPDPDQAVRLLLVEDNAVNRELVRTLLEPFGIGVDTANDGVAGVEAVRQGQYDLV
                     LMDIQMPGMDGLTATRRIRAMTEGAGSRTPIVAMTANVLPEQVANCLAAGMDDHLGKP
                     INPTKLLEAVARWSGRSHVEAATG"
     gene            1529..2107
                     /locus_tag="PLH31_11425"
     CDS             1529..2107
                     /locus_tag="PLH31_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQR89844.1"
                     /translation="MNKSMMLAIAGVLSISCAGTALAKMEHHAMGHGKTVMVGGAAMY
                     PTKNIVENAVNSKDHTTLVAAVKAASLVETLSSPGPFTVFAPTNAAFGKLPAGTVDTL
                     VKPENKATLTTILTYHVVAGRLTAADIEARAKAGGGKAVLTTVQGQPITVANGAHGWT
                     ITDAKGGMSHITIADVLQSNGVIHVVDTVLMP"
BASE COUNT          412 a          750 c          731 g          397 t
ORIGIN      
        1 gcttcacgac agaagaatac atgcagcggc cggcgaatgc gaacgtcagc ccggagaact
       61 ttgaacggat cgtcgccctg gtgcgcgatc tcgttccggg cggtgagccg cgcgtgttcg
      121 agtatgagag cgtgcacaag gatggctcgg ttatctggat cgagagcacc tattcgcgcc
      181 tggccggcga ggagggcgaa ctgctcgtca cctcgcgggt aaccaccgag cgcaagaagc
      241 tcgaaatcga actgaccaac gccctggagg cggcccagac cgccgcagcg gccaagtccg
      301 attttctggc caacatgacc cacgagctgc ggacgcccct gaacgccatc atcggctttg
      361 ctggcgtctt gcgcggctcc agcaccctgt cagagcgcga tgcgcggcat gtgggcctga
      421 tccaggacgc cagcaacacc ctgctcactg tcgtcaatga cgtgctcgac ttctcgcggc
      481 tggaggccgg cggcgtcgag ctggatccgc aacccttcga tccgcgggtc atggtcgcct
      541 cttgcgccgg gctgatcgac gggcaggcca gggccaaggg actggttctg caggtgtccg
      601 cgccagacga tcttcgggtc atgcaggccg atggaccaag actgacccag gtgctgctga
      661 atttcctgtc caacgccgtg aagttcacca attcgggcgg cgtgacggtc aatgtcaggc
      721 aggtcctgga cggcgatcag ggccttcttc gggtggaggt caccgacacc ggtatcggca
      781 tagccgaaca ccagctggat ggcgtcttcg accggttttc gcaggccgac gcagccgtgt
      841 ctcgccggtt tggcggcacg ggcctcgggc tggccatctc gcgccgcatc atcgagcaga
      901 tggacggcga gatcggtgtc gacagccgcg agggtgaggg ctcgcgcttc tggttcgaaa
      961 tccgggcgcc cttgtgcgag ctggcgcccg agacgttatt ggaagagccg acgctccccg
     1021 atcccgatca ggccgtacgt ctgctgttgg tcgaggacaa cgccgtcaat cgcgaactgg
     1081 ttcgcaccct gctcgagccg ttcggcatcg gcgtcgacac cgccaatgac ggcgtggcgg
     1141 gcgtcgaagc cgtgcgccag gggcagtatg acctggttct gatggacatc cagatgccgg
     1201 gtatggacgg cctgacggcc acgcggcgca ttcgggccat gaccgagggc gcaggctcac
     1261 gcacgccaat cgtcgccatg accgccaatg tcctgcccga gcaggtcgcc aactgtctgg
     1321 cggcgggcat ggatgatcac ctgggcaagc ccatcaaccc caccaagctg ctcgaggcgg
     1381 tcgcccgctg gtcgggtcgc agccacgtgg aagccgcgac cggctagaaa gtcgcgccaa
     1441 ttttcggatc cccgcgcatc cggttcagga tcgctcccgt aactgcatcg tcccagcaac
     1501 ggacagctac aattccggag acgacccaat gaacaagtcc atgatgctcg ccatcgctgg
     1561 cgtgctttcg atttcgtgcg ccggtacggc tctcgccaag atggaacatc atgccatggg
     1621 gcatggaaag actgtcatgg tcggcggtgc ggcgatgtat ccgaccaaga acattgttga
     1681 aaacgccgtc aactccaagg atcacacgac tctggtcgcc gcagtcaagg ctgccagcct
     1741 agttgagacc ctctccagcc ccggtccctt cacggtgttc gcgccgacca acgccgcctt
     1801 cggcaagctc ccggccggca cggtggacac cctggtcaag cccgagaaca aggcgaccct
     1861 caccaccatc ctgacctatc acgtggtggc cggacgcctg acggcggctg acatcgaagc
     1921 ccgggccaag gccggcggcg gcaaggctgt tctgaccacc gttcagggcc agcccatcac
     1981 cgtggccaat ggcgctcatg gttggacgat cacggacgcc aagggcggca tgtcgcacat
     2041 caccatcgct gacgtgctgc agtccaacgg cgtgatccac gtcgtcgaca ccgtcttgat
     2101 gccctaaacg tcccggctcc cgggccgcct tgatggcggc ccgggaccgc tacttctcgg
     2161 gagacgcggg ctttgcggcc gcctcgcccc gggagtccgg aatgtagagg ttggtgatca
     2221 tccgggccac catgccgccc ggctgaaagc gattgccgta cgccttgcac gggtctgtct
     2281 tggcgtcgat
//
LOCUS       DAOMEO010000508         2289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_4988_length_2289_cov_1.847807, whole genome shotgun sequence.
ACCESSION   DAOMEO010000508 DAOMEO010000000
VERSION     DAOMEO010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_2289_cov_1.847807"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLH31_11430"
     CDS             <1..324
                     /locus_tag="PLH31_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HQR89845.1"
                     /translation="CLQLCLSLDLRAPKTKRASAYELLAMINQRVWLGHFEVWTEDGE
                     VVFRHALALPSGERPTMAQAASMIDAAVEAADRFYPAFDFLLQGAKTPDEAMAACMFE
                     TVGQA"
     gene            453..1221
                     /gene="proC"
                     /locus_tag="PLH31_11435"
                     /pseudo
     CDS             453..1221
                     /gene="proC"
                     /locus_tag="PLH31_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
     gene            1288..>1550
                     /locus_tag="PLH31_11440"
     CDS             1288..>1550
                     /locus_tag="PLH31_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR89846.1"
                     /translation="MTASEDILDRAAAAALALASDKPWHHIALRDIALKAEIPFAELY
                     ALASGKGAVLAHLSTRFDRAALGTVSADEASPHDRLFDAAMARL"
     assembly_gap    1551..1560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1561..1810
                     /locus_tag="PLH31_11445"
     CDS             <1561..1810
                     /locus_tag="PLH31_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR89847.1"
                     /translation="IAIAASEGVVVSALRFPKTARAILEAGGVEATPVRLAAMTAVWA
                     RTVQVWRDDEGALNRTMAELDKRLKQMAERLGKLGAGF"
     gene            complement(1928..>2289)
                     /locus_tag="PLH31_11450"
     CDS             complement(1928..>2289)
                     /locus_tag="PLH31_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR89848.1"
                     /translation="SAVPPDLAVELRPNAFKRALANLIDNAIAYGDHVRVTAAERITG
                     GVEIVVEDDGPGIPEDRYEEAFKPFSRLDESRNQNEKGVGLGLAIARDMARGLGGDLV
                     LERSAMGGLRAVMRLPG"
BASE COUNT          317 a          830 c          764 g          368 t
ORIGIN      
        1 tgcctgcagc tgtgcctgtc gctggacctg cgcgccccca agaccaaacg cgccagcgcc
       61 tatgagctgc tggccatgat caaccagcgc gtctggctgg gccatttcga ggtctggacc
      121 gaggacggcg aagtcgtgtt ccgtcacgcc ctggccctgc cctcgggcga gcgcccgacc
      181 atggcccagg ccgcctcgat gatcgacgcg gctgtcgaag ccgccgaccg cttctatccg
      241 gccttcgact tcctgctgca gggcgccaag accccggacg aggccatggc cgcctgcatg
      301 ttcgagacgg tgggtcaggc ataatcagct tgtcatcccg gccgaaagcc cgcatcgcgg
      361 gctgaagagc cgggaccgcg acaggcgcta gagcttccgg cggtcccgga tcggcctttc
      421 aggccgtccg ggatgacagc taggagtttc tcatgacccc catcctcctc ctcggcgccg
      481 gtcgcatggg cggcgcgctg atccagggct ggcgcgaggc tggcgccttc tccgccgccg
      541 acctgatcgt ccgtgatccc aatgtcgata ccgccgcctt tgaaggcgcg ctggttaatc
      601 cgccgctcga agccctgtcg gccgcgaaaa cggtcctgct ggccgtgaaa ccgcagatct
      661 ggcgcgaggc gatccaggac gtggtcccgc atctggctcc cgacgccgtc atcgtctcga
      721 tcgccgctgg cgtccgggcc gccgacatct ccgaagcctt cggcggccgc gctgtcgccc
      781 gggtgatgcc caccaccgcc gtcgccatcg gccgcggtac ggccagcgtc tatgccgaca
      841 cggcccaagc ccgccctgct caatccggtc gccaccgtgg tggatctgga cgacgaagac
      901 ctgatgcatg ccgccaccgc cgtctcgggc tcggccccgg cctatcttta cgccttcgtc
      961 gaggcgctgg aagcggccgg cgctggcgcc ggtctcgacc ctgccgcttc ggcgaaactg
     1021 gcccgcgcca ccatcatcgg cgcggcggcc ctgatggagg ccggcggtga agagccctcc
     1081 gagttgcgca agcaggtcac ctcgcccggc ggcacgacgg cggcggcgct cgcagtgttg
     1141 atgggtgagg gcgggttcgg cgacctgctg ccccgggcgc tggaggcggc gatcgcgcga
     1201 tcgaaggaac tgggcggcta aggtcggcgc tcgcccacga cgcgcccagc cttgtcagga
     1261 ccgccttcga cagccatctg agtgaccatg accgcctctg aagacatcct cgaccgcgcc
     1321 gccgccgccg ccctggccct ggcgtccgac aagccctggc atcacatcgc tctgcgcgat
     1381 atcgccctga aggccgagat cccgttcgcc gagctttatg ccctggcctc gggcaagggc
     1441 gcggtgctgg cccatctgtc cacgcgcttc gaccgcgcgg ctctgggcac tgtctcggct
     1501 gacgaggcct cgccgcacga tcgcctgttc gacgcggcca tggcgcggct nnnnnnnnnn
     1561 gatcgccatc gcggcgtcgg agggcgtggt ggtctcggcc ctgcggtttc ccaagaccgc
     1621 gcgagcgatc cttgaggccg gcggcgtcga ggcgacgccg gtgcggctgg ccgccatgac
     1681 ggcggtttgg gcgcgcacgg tgcaggtgtg gcgcgatgac gagggcgcgc ttaaccgcac
     1741 gatggccgag ctcgacaagc gtttgaagca gatggccgag cggctgggga aattgggcgc
     1801 cgggttctag ctgctcccct tttgggggag ctgtcgcgga gcgactgagg gggccatcgc
     1861 ggcctaagtc cagctacccc cctccggccc tctgggccac ctcccccaga gggggaggat
     1921 ctaccaccta ccccggcaac cgcatcaccg cccgcagccc ccccatcgcc gaccgctcga
     1981 gcacaaggtc cccgcccagc ccgcgcgcca tgtcgcgggc gatggccagg cccaggccga
     2041 cgcccttttc gttctggttc cgcgactcat ccaggcggct gaacggcttg aaggcttctt
     2101 cgtagcggtc ctctgggatg cccggcccgt cgtcctcgac aacgatctcg accccgccgg
     2161 tgatccgctc ggccgccgtg accctgacat ggtcgccata ggcgatggca ttgtcgatca
     2221 ggttggccag ggcgcgcttg aaggcattgg ggcgcagttc gacggccagg tccggcggca
     2281 cggcggact
//
LOCUS       DAOMEO010000509         2284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5006_length_2284_cov_2.589502, whole genome shotgun sequence.
ACCESSION   DAOMEO010000509 DAOMEO010000000
VERSION     DAOMEO010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_2284_cov_2.589502"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="PLH31_11455"
     CDS             complement(<1..>1007)
                     /locus_tag="PLH31_11455"
                     /inference="COORDINATES: protein motif:HMM:NF023776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HQR89849.1"
                     /translation="GRWPNDQDILAYFTRPTRSVNHRAIAEIRKRTKHTPVKPASSDD
                     LDLFLASWPDVDPETGLSLQGDELLIKAREAMIAAVHIFNSAGLTFRAELFIVTAVIG
                     WTYLLHAWFKREGIDYRHTKTEKGETVVLKTPNGADKYWELAQCLRHVRSPIERGVAD
                     NLSFLLDLRHEIEHRSTNRIDDAVSAKLQACCINFNDIIKVQFGAQYALERRLPIALQ
                     FVTFSPDQRALLKKASSLPRHVETMMDGFEQNLTPEQQADPRFAYRVFMIHKTANRAP
                     GADLAVELIPPGSDVDDKFNMVLKEVEKRKYLPAEVVAKVRAEGWNGFTMDSHTRLWQ
                     R"
     assembly_gap    1008..1017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1164..>2284)
                     /locus_tag="PLH31_11460"
     CDS             complement(1164..>2284)
                     /locus_tag="PLH31_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89850.1"
                     /translation="RAFPITAADFGNVNPNTGTAPIFRSRRDMALTAAIYGRLPVLVD
                     RSSSEPVAAWPVRYATMFHMTNDSHLFRTRAELEESEGAWSTGGNRFQSQSGEWVPLY
                     AGRMIHQFDHRAASVVVNSANIHNAALSGDVTAEMKADPAFLPAPQYWLLDQGSMDGP
                     AIAFRDIARSTDARTVIAAMLPHRAAGNTAPLILSDTAAPDQALLLANLNAIVLDYVA
                     RQKAQSTHLNWYIVEQFPVVPLKDYARKFGPKSAADIVRDIVLELSYTAHDLAPFARG
                     LGYVDADGEVHPPFVWDDDRRLMLRAKLDALYFILYGVFDPADSTQSREDIHYIYSTF
                     PIIEREEVAKWGSYRSRDLALAWVNALMAGQPDAEIAG"
BASE COUNT          443 a          644 c          718 g          469 t
ORIGIN      
        1 ggcgttgcca aagcctggtg tgactatcca tagtgaagcc gttccaaccc tctgctctga
       61 ccttggcgac cacctcggcc ggcagatact tgcgcttctc aacttccttc aggaccatgt
      121 tgaacttgtc gtcgacatcg gaaccgggcg ggatcaactc gactgccagg tcagcgcccg
      181 gagcacgatt tgcggtcttg tggatcatga agacacgata ggcgaagcgt ggatcggcct
      241 gctgttcggg cgtcagattc tgctcgaacc cgtccatcat cgtctcgacg tgacgaggca
      301 ggctgctggc cttcttgaga agagcgcgct ggtcggggct gaacgtgacg aactgaagcg
      361 cgatcggaag gcggcgttcc agagcgtatt gcgccccgaa ctgcaccttg atgatatcgt
      421 tgaagttgat gcagcaggcc tgaagtttgg cgctgacggc atcgtcgatc ctattggtcg
      481 agcgatgttc gatctcgtgt cgcagatcga gcaggaagga caggttgtcg gccaccccgc
      541 gctcgatcgg gctgcgtacg tgcctcaggc actgggccag ttcccagtat ttgtcggctc
      601 catttggagt cttgaggacg accgtctcgc ccttctcggt cttggtgtga cgatagtcga
      661 tgccttcgcg cttgaaccat gcgtgcagca aataggtcca gccgatgacg gcagtaacga
      721 tgaacagttc ggcgcggaac gtcaggccgg cgctgttgaa gatgtgaacc gccgcgatca
      781 tggcctcacg cgccttgatc agcagttcgt ctccctgaag gctcaggccg gtctcggggt
      841 ccacatcggg ccaggacgcg aggaacaaat ccaagtcgtc cgatgaagcc ggtttgacgg
      901 gtgtgtgttt agtcctcttc cgtatttcgg caatcgcccg gtgattcacc gatcgagtgg
      961 ggcgcgtgaa atatgcaagg atgtcctggt cattcggcca ccgcccgnnn nnnnnnncag
     1021 ggcgatctcc caccgttcca gagtattgcc ccgttttcgg attatcccca gcttggcggg
     1081 tgccggcgcc gcagctacgg acacagcagg agccggagca gccacggcca cggcgggtgg
     1141 aatacggccg gtaaagggtg ctttcatcca gctatctcgg cgtcaggctg acccgccatc
     1201 agggcgttga cccatgccag agctagatca cggctgcggt aggaacccca cttggcgact
     1261 tcctcgcgct caataattgg gaacgtcgag taaatgtagt ggatgtcttc gcggctctgc
     1321 gtggagtcgg cggggtcgaa gacgccgtag aggatgaagt agagcgcatc cagcttggcc
     1381 cgcaacatca gccggcggtc atcgtcccaa acgaaagggg gatggacttc gccatcggca
     1441 tccacatagc ccagaccgcg cgcgaagggg gccagatcat gcgcggtgta gctgagttcc
     1501 agaactatgt cgcgaacgat gtcggcggca gactttggac cgaactttcg cgcgtagtcc
     1561 ttcaatggga cgacgggaaa ctgctccacg atgtaccaat tgagatgggt gctttgagcc
     1621 ttctgccggg ccacgtagtc gagaacgatc gcgtttaggt ttgcaagcag aagggcttga
     1681 tcgggcgcgg ctgtgtccga gagtataagc ggcgcggtgt ttccagccgc gcggtgaggc
     1741 agcattgcgg ctataacagt gcgcgcgtct gttgatcgcg caatgtccct gaacgcgata
     1801 gccgggccgt ccatcgatcc ttgatctaga agccagtatt gcggggcagg aagaaacgcg
     1861 gggtcggcct tcatctccgc cgtcacatcg ccactcaagg cggcgttgtg aatgttggcg
     1921 gagttaacga cgacggaggc ggcgcggtga tcgaactggt ggatcatcct acctgcgtag
     1981 agcggcaccc actctcccga ctgggactga aatcggtttc cgcccgtact ccaagcgcct
     2041 tcgctctcct ccagttcggc acgagtgcgg aagagatggc tgtcgtttgt catatggaac
     2101 attgttgcgt agcgcactgg ccaagccgcg accggctcgc tgctgctgcg atcaacgagc
     2161 accggtaggc ggccgtagat cgcagcagtt aaagccatgt cccgccgcga acggaaaatc
     2221 ggcgcggttc cagtatttgg attcacgttc ccaaaatcag ccgccgtaat ggggaaggcg
     2281 cgat
//
LOCUS       DAOMEO010000510         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5010_length_2283_cov_2.095601, whole genome shotgun sequence.
ACCESSION   DAOMEO010000510 DAOMEO010000000
VERSION     DAOMEO010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_2283_cov_2.095601"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLH31_11465"
     CDS             complement(<1..498)
                     /locus_tag="PLH31_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQR89851.1"
                     /translation="MRLSAFAPAKVNLFLHVGGPDAEGYHPISSLMVFADVGDVVALQ
                     ASDAPGFETTGPFGSEIPAGDDNLVVRAVRAFHAGAAAPLPPFRLLLDKRLPIAAGLG
                     GGSSDAGAALRLLRDALAPGLSDKALEVMACSLGADGAACLWGLPVIAEGRGERLSPA
                     PSLPPL"
     gene            complement(540..2264)
                     /locus_tag="PLH31_11470"
     CDS             complement(540..2264)
                     /locus_tag="PLH31_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89852.1"
                     /translation="MRFKVLLAAVSLLALSACASTQDVALRGPVSNSYSFFDTRSSYG
                     QFLAGQAALRDGRTREAADYFGVAGVLGDDPGVIGERTFTALLLSGDVTRAAQIAPRG
                     EDASEAVKRLASLTRVVELLASGKGKEAQALLKAEPMGFPHRQAAALLGPWTAAAAGD
                     AEGATVQPSLQNDKLVQYFGQLGQARLYERARRYEEAETDYKALTSLSNTAGLFILDY
                     GAFLERRKRHADAVALYDQALSADPADPGLQTARARAKAKSAPPPMQTIKQGAAQSLV
                     ACAATFAGERQSQFALAYLRLALRLDPEREEAWLLVGDLLNQAEDHRGAIDAFGRIPV
                     ASPQYATAQSKLAWTYQQSGDKPRALEIARQSVAAAPDDRDAQIALADLLRANERWDE
                     SVAVLTPLIAREVDRPDWRVLYLRGIALERAGRWAEAERDLSAALKINPDEPEMLNFL
                     GYSWIDRNERLPEALAMVQKAVAARPQSGAMLDSLGWAYYRLGNYTLAVEKLERAVEM
                     EPGDPDVNGHLGDAYWRAGRQIEARFQWRRVLSLEPDAKQKAEAEAKLKDGLGAAGSA
                     APSTIARN"
BASE COUNT          387 a          760 c          789 g          347 t
ORIGIN      
        1 cagcggcggc aggctggggg ccggcgacaa acgctcgcca cggccctcgg cgatcaccgg
       61 aaggccccag agacacgctg cgccatccgc gccgaggctg caggccataa cctcgagggc
      121 tttgtctgac aggccaggag ccagggcgtc gcggagcaga cgcagggccg ctccggcgtc
      181 actggagccg ccgcccaggc ccgcagcaat cggcagacgc ttgtcgagca gcaagcggaa
      241 cggcggcagg ggcgccgccg cgccggcgtg gaaggcccgc accgcacgca ccaccaggtt
      301 gtcgtcaccc gccgggatct cggaaccgaa gggcccggtg gtttcgaagc cgggggcgtc
      361 gctggcctgg agcgcgacca catcgccgac atcggcgaag accatcaggc tggaaatggg
      421 atgatagcct tcggcgtcag ggccgccgac gtgcaggaac aggttgacct tggctggggc
      481 gaaggcggat aggcgcatgg aagaccccag gacggacgag cgagacccgt cccgataact
      541 cagttacggg cgatggtcga cggagccgca gatccggcgg cgccgagacc atctttcagc
      601 ttggcctcgg cctcggcctt ttgcttggcg tcaggttcga gactgagcac gcggcgccac
      661 tggaaccggg cctcgatctg tcggcccgcg cgccaatagg cgtcgccgag atgaccgttc
      721 acgtccgggt cgcccggttc catctcgacg gctcgttcca gcttctcgac ggccagcgtg
      781 tagttgccca ggcgatagta ggcccagccc agagagtcga gcatggctcc ggattgcggg
      841 cgcgcggcga cggccttctg gaccatggcc agggcttcgg gcaggcgctc attgcgatca
      901 atccacgaat agccgaggaa attcagcatc tcgggctcgt cgggattgat cttcaacgcc
      961 gcgctgagat cgcgctcggc ttcggcccag cgcccggccc gttccagggc gatgccgcgc
     1021 aggtaaagca cgcgccagtc cggacgatca acctcgcgcg cgatcagggg ggtcaggacc
     1081 gccacagatt catcccagcg ctcattggca cgcaacaggt cggccagggc gatctgggcg
     1141 tcgcggtcat ccggcgcggc ggccacggac tgacgcgcga tttcaagggc gcggggcttg
     1201 tctccgctct gttgataggt ccaggccagc ttgctctggg ctgtcgcata ctggggcgag
     1261 gcaaccggga tgcggccaaa ggcgtcgatg gcgccgcgat gatcctcagc ctggttcaga
     1321 aggtcgccga ccagtagcca ggcctcttca cgttccgggt ccagccgcag ggccaagcgc
     1381 agataggcca gggcaaactg gctctgccgt tcccccgcaa acgtcgctgc gcaggcgacc
     1441 aggctctgag ccgcgccctg tttgatcgtc tgcatcggcg gtggggcgct cttggccttt
     1501 gcgcgcgcgc gggcggtctg cagtccgggg tctgcaggat cggcgctcag agcctgatcg
     1561 tacagggcga ccgcatcggc atgacgcttg cggcgctcca ggaatgcccc gtagtcgaga
     1621 atgaaaaggc ctgccgtatt cgataaggat gtcagcgcct tgtagtctgt ttcggcctct
     1681 tcatagcgcc gcgcgcgctc atagaggcgg gcctgaccca gctggccaaa atactgcacc
     1741 agcttatcgt tctgcaggct gggctgcacc gtcgcgccct cggcgtcgcc cgcagcggcg
     1801 gccgtccagg gccccagcag cgccgcggcc tgacgatgcg ggaagcccat gggctcggcc
     1861 ttcaaaaggg cctgggcctc cttgcccttg cctgaagcga gcagttccac aacgcgcgtc
     1921 agggatgcca gccgcttgac ggcttcgctc gcgtcttcgc cgcgaggcgc aatctgggcg
     1981 gcgcgggtca catcgcccga cagcagcagg gctgtgaagg tccgctcgcc gatgaccccg
     2041 ggatcatcgc ccaggacgcc tgccacgccg aaatagtcgg ccgcctcgcg cgtgcggcca
     2101 tcgcgcagcg ccgcctgtcc ggccagaaac tgaccgtagg aactgcgagt atcgaagaac
     2161 gagtagctgt tcgacaccgg gccgcgcagg gcgacatcct gggtcgaggc gcaggccgag
     2221 agagccagga gggacacggc ggcgagaagg actttaaaac gcatcccgcc accctgttgg
     2281 gtt
//
LOCUS       DAOMEO010000511         2280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5019_length_2280_cov_2.484494, whole genome shotgun sequence.
ACCESSION   DAOMEO010000511 DAOMEO010000000
VERSION     DAOMEO010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_2280_cov_2.484494"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1128
                     /gene="era"
                     /locus_tag="PLH31_11475"
     CDS             163..1128
                     /gene="era"
                     /locus_tag="PLH31_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQR89853.1"
                     /translation="MTDTTSQNTPLATRAGFAAIIGAPNAGKSTLVNRMVGAKVSIVT
                     QKVQTTRFPVRGVAMHGAAQIILVDTPGIFSPRRRLDRAMVRSAWSGSEEAEVTVHLV
                     DVQAELASNINKATPAEHRSVQDVQTIIEGLKSANRQVILALNKIDGVKRDTLLAVTQ
                     ELFDTGVYSEVFMISAATGAGVDDLLERLAGMMPESPWLYPEDQTADLPARLLAAEIT
                     REKIYLRVHEELPYAASVETTAFVERADGSARIEQTIYVERDGQRAIVLGAGGQTLKW
                     IGQASREELCDILDRKVHLFLHVKVKENWAEERGLFSDMGLDFDV"
     gene            1206..1937
                     /gene="recO"
                     /locus_tag="PLH31_11480"
     CDS             1206..1937
                     /gene="recO"
                     /locus_tag="PLH31_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQR89854.1"
                     /translation="MEWEDEALVLSARIHGETGAIVELLTEQHGKFAAHVAGAASRRM
                     KPFLQPGSRVIARYRARMSDQLGTATLEPMGEGVASLFDDPLALAGLSSAASLAAGAL
                     PEREAHPGAFHALEALIGVLQIPDIWPAVYVRYEAGLLQELGFGLDLSKCAATGTFDD
                     LIYVSPRTGRAVSRAAGEPYKDKLLPLPPFLLSAQGGLAEGDVKAGLEITGHFLEQFV
                     FGPLNRPLPPTRMWLLDRLGEAGRL"
     gene            1995..>2280
                     /locus_tag="PLH31_11485"
     CDS             1995..>2280
                     /locus_tag="PLH31_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HQR89855.1"
                     /translation="MSILLDRYALSRRRALAAFGGVAATALSIPLMTGQALAQPVFSI
                     YPFQLGVASGDPQADGFVIWTRLAPQPLEIGHGMPSAPMEVSWEVAADAGF"
BASE COUNT          360 a          809 c          736 g          375 t
ORIGIN      
        1 cgggcttgtt catctgcgga tgctgccacc cagcgcagat gcccgcaatc cacccatctc
       61 agaagttggt tgcgtccgct catcacctcg aaacgccatc ccccgaccgc catccccatc
      121 agggggagga atcccggccc cgctgtgcta cacgcacgcg caatgactga caccacctca
      181 caaaacaccc cactcgccac ccgcgcgggt ttcgccgcca tcatcggcgc cccgaacgcc
      241 ggcaagtcca ccctggtcaa ccgcatggtc ggggccaagg tctcgatcgt cacccagaag
      301 gtccagacga cgcgctttcc cgtgcgcggc gtggccatgc atggcgccgc ccagatcatc
      361 ctggtcgaca cccccggcat cttcagcccg cgccgccgcc tggaccgcgc catggtccgc
      421 tccgcctgga gcggttcgga agaggccgag gtcaccgtgc acctggtcga cgtgcaggcc
      481 gaactggcct cgaacatcaa caaggccacg cccgccgagc accgctcggt gcaggacgtg
      541 cagaccatca tcgagggctt gaagagcgcc aatcgccagg tgatcctggc gctcaacaag
      601 atcgacgggg tcaagcgcga caccttgctg gccgtgaccc aggagctgtt cgacaccggc
      661 gtctattccg aggtcttcat gatcagcgcc gccaccggcg cgggcgtcga cgacctgctg
      721 gagcgtctgg ccggcatgat gccggaaagc ccctggctct atccggaaga ccagaccgcc
      781 gacctgccgg cccgcctgct ggccgccgag atcacccgcg agaagatcta tctgcgcgtc
      841 cacgaggagc tgccctacgc cgcctccgtc gagacgaccg ctttcgtgga acgcgccgac
      901 ggcagcgccc gcatcgagca gaccatctat gtcgaacgcg acggccagcg cgccatcgtg
      961 ctgggcgcgg gcggccagac cctgaagtgg atcggccagg cctcgcgcga ggaactgtgc
     1021 gacatcctgg atcgcaaggt gcacctgttc ctgcacgtga aggtcaagga aaactgggcc
     1081 gaagagcgcg gcctgttcag cgacatgggc ctggacttcg acgtctgacc tcgcgctcga
     1141 acacttgact tcgccggccg caccctgtcc ttggaccgtg aacagacagg gaatatcgcg
     1201 gtcgcatgga gtgggaagac gaggccctgg tgttgtcggc tcgcatccat ggcgagaccg
     1261 gcgccattgt cgaactgctg accgagcagc acggcaagtt cgcggcccat gtggcgggcg
     1321 cggcctcgcg gcgaatgaaa ccgtttctgc agccgggctc gcgggtcatc gcccgctatc
     1381 gcgcccggat gtccgaccag ctggggacgg cgacgctgga gccgatgggc gagggggtgg
     1441 cgtcgctgtt cgacgatccc ctggccctgg ccggcctgtc gtcggccgcc tcgctggccg
     1501 ccggcgccct gcccgagcgt gaggcccatc ccggcgcctt tcatgcgctg gaggccctga
     1561 tcggcgtgct gcagatcccc gacatctggc cggcggtcta tgtgcgctat gaggccggtc
     1621 tgttacagga gctgggcttt ggcctggatc tgtccaaatg cgccgccacc ggcacgttcg
     1681 atgacctgat ctatgtcagc ccccgcaccg gccgcgccgt cagccgcgcg gcgggcgagc
     1741 cctacaagga caagctgctg ccgctgccgc cgtttctgct ctcggcccag ggcggactgg
     1801 cggagggcga cgtgaaggcg gggctggaga tcaccggtca cttcctggag cagttcgtgt
     1861 ttggccctct gaaccggccg ctgccgccca cccggatgtg gctgctggat cggctgggcg
     1921 aggccgggcg actttaggcc taacgatccg tcacaaagtc gggatatcgg gcaagcctgt
     1981 ccgaggagtc gccgatgtcc atcctgcttg atcgctacgc cctgtcgcgc cgtcgcgccc
     2041 tggccgcctt tggcggcgtc gccgccaccg ccctgtcgat cccgctgatg acgggccagg
     2101 ccctggccca gccggtgttc tcgatctatc cgttccagct gggcgtcgcc tcgggtgatc
     2161 cgcaggcgga cggttttgtg atctggaccc gtcttgcgcc ccagcccctg gagattggcc
     2221 acggcatgcc cagcgcgccg atggaagtct cctgggaagt cgccgccgac gcgggcttta
//
LOCUS       DAOMEO010000512         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5025_length_2279_cov_2.290018, whole genome shotgun sequence.
ACCESSION   DAOMEO010000512 DAOMEO010000000
VERSION     DAOMEO010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5025_length_2279_cov_2.290018"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLH31_11490"
     CDS             complement(<1..735)
                     /locus_tag="PLH31_11490"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HQR89856.1"
                     /translation="MPVDPPAADRTFAPIHQIRGQSVVLDDAIAALFGVETRRLNEQV
                     RRNAARFEGYAFQLTAEEFARLISQNAISNSRGGRRTRPWAFTEHGVVMAATVLKSDS
                     AIAAMKMVVEVFVSARRRPDTSRQIANDVSVGNALIPRLTQAMAAVLDSVVSQRHGTS
                     VREEAQDLLDRSIAHLKDRLSRAGLENEELAARATKLLAEAEASKATAAKTQAEAGEI
                     ELGVLARKLQLVIEAERAMTGGDIDRF"
     gene            complement(797..>1067)
                     /locus_tag="PLH31_11495"
     CDS             complement(797..>1067)
                     /locus_tag="PLH31_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR89857.1"
                     /translation="MGLVGETRPNAGTHSNRIHIVAARNVVRVADPLDDPTERIERLW
                     VPAAEALRMALAGEMTVGMQVASLLRGLVQAGVATITLANPAEED"
     assembly_gap    1068..1077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1078..1375)
                     /locus_tag="PLH31_11500"
     CDS             complement(<1078..1375)
                     /locus_tag="PLH31_11500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR89858.1"
                     /translation="MADGKWRVTASRYVHKDRWISVRADDCVTDEGAVVAPYYVLEYP
                     DWVEVIALDADDNVLLVRQYRHGLGGFSVELPAGAMEPTDADPIAAATRELLEEA"
     gene            complement(1392..1973)
                     /gene="rimM"
                     /locus_tag="PLH31_11505"
     CDS             complement(1392..1973)
                     /gene="rimM"
                     /locus_tag="PLH31_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041539175.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQR89859.1"
                     /translation="MSAPSPANLILVGRVAGGFGVRGEVKITTYTEDPKSLAGFKALT
                     RQDGSPALTILSARVAKEGLVARCQGIETKDAADALRGLRLYVPRESLPEPDEDEFYL
                     TDLVGLPVRHIADNALLGSVKSVQNFGAGDLLEIQPALGGQTWFLPFTRQAVPEVKIA
                     ERLILADPPALVGEPEGPAQEAEDADIVERNYD"
     gene            complement(2028..>2279)
                     /locus_tag="PLH31_11510"
     CDS             complement(2028..>2279)
                     /locus_tag="PLH31_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89860.1"
                     /translation="QREEDRKAADVKAAEEAAAAAAQAAEDAKAAAEAEAAAAAEAAA
                     APAPEPVAEEAPVEAAAEEAPAAEAAAEDAPAEAEKTEG"
BASE COUNT          354 a          756 c          756 g          403 t
ORIGIN      
        1 gaaccgatcg atatcgcccc cggtcatcgc ccgctcggct tcgatcacca gttgcagctt
       61 gcgcgccagg acccccaatt cgatctcgcc ggcctcggct tgggtcttgg cggcggtcgc
      121 cttgctggcc tcggcttccg ccagaagctt ggtcgcccgc gccgccagtt cctcattctc
      181 aaggccggcg cgcgatagcc gatccttgag atgggcgatg gagcggtcca gcaggtcctg
      241 agcctcctcc cggacggagg ttccatgacg ctggctcacc accgagtcga gcaccgccgc
      301 catcgcctgg gtcaacctgg ggatcagggc gttgccgacg ctcacatcgt tggcgatttg
      361 ccgtgaggta tccggtcttc gacgcgcgct gacgaagacc tcgaccacca tcttcatcgc
      421 ggcgatggct gaatcggatt tcagaacggt cgccgccatg accacgccgt gttcggtaaa
      481 ggcccagggc cgtgtccgtc gcccacctcg ggagtttgat atcgcatttt gcgatatcaa
      541 acgggcgaac tcctcggccg tcagttgaaa ggcatagccc tcgaaccgtg cggcattgcg
      601 gcggacctgt tcgttgaggc gccgggtctc cacaccgaag agagcggcga tcgcgtcgtc
      661 aagaacgaca ctttgccctc ggatttgatg gataggggca aacgtcctgt cggcggcggg
      721 tggatcgacg ggcatggggc tcaacagcgc attgagggtg gtggctcctc cacactctct
      781 ttgattgagt tcgagatcaa tcctcttcgg ccgggttcgc cagggtgatg gtcgccacac
      841 cggcctgaac caggccgcgc agcagcgaag cgacctgcat gcccacggtc atctcgccgg
      901 ccagggccat gcgcagggct tcggccgcag gaacccagag ccgctctata cgctcggtcg
      961 ggtcgtccag cgggtcggca accctgacca cgttgcgcgc cgcaacgatg tggatgcgat
     1021 tggaatgggt gccggcattg ggccgcgtct caccgaccaa acccatcnnn nnnnnnncgg
     1081 cctcctccag cagttcgcgg gtcgcggcgg cgatcggatc ggcgtcggtc ggctccatgg
     1141 cgccggccgg caactcgacg gagaaaccgc ccaggccatg gcggtactgg cgcaccagca
     1201 ggacgttgtc gtcggcgtcc agggcgatga cttcgaccca gtccggatac tccagcacgt
     1261 aatagggggc gaccaccgcg ccttcgtcgg tgacgcagtc gtcggcgcgg acgctgatcc
     1321 agcgatcctt gtgaacatag cgcgaggcgg tgacccgcca cttcccgtcg gccatgcccg
     1381 atactcctgg atcagtcgta gttgcgctcg acgatgtcgg cgtcttcggc ctcttgcgcc
     1441 gggccttccg gctcgccgac cagagccgga ggatcagcca ggatcaggcg ttcggcgatc
     1501 ttcacctcgg gcacggcctg ccgggtgaag ggcaggaacc aggtctggcc gccgagggcg
     1561 ggctggatct ccagcaggtc gccggcgccg aagttctgca cgctcttgac cgagcccagc
     1621 agggcgttgt cggcaatatg gcgcacgggc aggccgacca gatcagtcag atagaattcg
     1681 tcttcgtccg gctccggcag cgactcccgg gggacataca ggcgcaggcc gcgcagggcg
     1741 tcggcggcgt ccttggtctc gatcccctgg cagcgggcga cgaggccttc cttggccacg
     1801 cgggccgaaa ggatcgtcag ggccggcgag ccatcctggc gcgtcagcgc cttgaaaccg
     1861 gccaggctct tggggtcttc ggtataggtc gtgatcttca cctcgccgcg cacgccaaag
     1921 ccgccggcga cgcggccgac caggatcaga ttggcgggag acggagcgct catgggtagc
     1981 gacatcgccg ccccgatccg aagaccgggg cggcaacgca aaaggcctta gccttcggtc
     2041 ttttcggctt ccgccggagc gtcttcagca gcggcttcag cggccggagc ctcttcggcg
     2101 gcggcttcga ccggcgcttc ttcagcaacc ggctccggag cgggagcagc agcggcttca
     2161 gcagcagcag cggcttcggc ttccgcggcg gccttggcgt cttcagcggc ttgagcggcg
     2221 gcggcggcgg cttcttcagc agccttcacg tcggcggcct tgcggtcttc ttcgcgttg
//
LOCUS       DAOMEO010000513         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5031_length_2278_cov_2.626181, whole genome shotgun sequence.
ACCESSION   DAOMEO010000513 DAOMEO010000000
VERSION     DAOMEO010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_2278_cov_2.626181"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2278
                     /gene="pheT"
                     /locus_tag="PLH31_11515"
     CDS             <1..>2278
                     /gene="pheT"
                     /locus_tag="PLH31_11515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQR89861.1"
                     /translation="AGLEVEHVTDPATKLAAFTVAKIVEAVQHPNADRLRVCQVDTVD
                     GMKEIVCGAPNARAGLTTIYAPIGAYVPGLGVTLVEKPVRGVISNGMLCSASELEAAS
                     ESDGIMELPDDLAVGTPAATALGMEAVIDFEVTPNRPDWLGVAGIARDLAAAGVGTLK
                     DLSIASVPGTFPCPITVKVDGEACPVFAGRLIKGVRNGQSPKWLQDRLLAIGLRPISA
                     LVDITNLITYDRARPLHVYDVAKLSGTEISTRLGRHGQHGDEHLIALDGKTYELTPDM
                     CVIADASGERPIGLGGVMGGESTGCSEETVDVFVESAWFEPIRIAQTGRTTGINSDAQ
                     YRFARTVDTHSLVPGIELATRLILDLCGGEPSEIVVAGQAPAAPAGFAFEPDYVEQLS
                     GLAVTPEQTRQILSDLGFDIVLGSPWTVTPPTFRRDVEGKADLVEEVARIAGYGALPS
                     TALPEVPRAVGGVLTAKQARGRLARRALAAAGYAEALTWSFTNQKIATLFGGGAAELV
                     LANPIASDLDCMRPSLLPNLIEAAGRNARKGFPDAALFEIGPTFHGDRPADQRTVIAA
                     IIAPHAPRGWAKTAQDDLFTVKADLLALLEELGAPVDKLQTAQGSASSWWHPGRSARL
                     QLGPKAVMAEFGELHPAVLKALDVAGPVYGFEITLEAIPEPKKKAHKSKAAFSPSPLM
                     PLTRDFAFLVETAKPAGDLVKAALGADKALITAVRVFDVYDGAGVPDGFKSVALEVVV
                     QPREATLTDADIEALSGRI"
BASE COUNT          346 a          846 c          749 g          337 t
ORIGIN      
        1 ggccggcctc gaggtcgagc acgtcaccga tccggccacc aagctggccg ccttcacggt
       61 cgccaagatc gtggaagctg ttcagcatcc caacgccgac cgcctccgcg tctgccaggt
      121 cgacaccgtc gacggcatga aggagatcgt ctgcggcgcg cccaacgccc gcgcgggcct
      181 gaccaccatc tacgctccca tcggcgccta tgtgccgggc ctgggcgtca ccctggtcga
      241 gaagccggtg cgcggcgtga tctccaacgg catgctgtgc tcggcctccg agctggaagc
      301 cgccagcgag agcgacggca tcatggaact gccggacgac ctggccgtcg gcacgcccgc
      361 cgccacggct ctgggcatgg aagcggtcat cgacttcgag gtcaccccca accgccccga
      421 ctggctgggc gtcgccggca tcgcccgcga cctggccgcc gccggcgtcg gcacgctgaa
      481 ggacctttcc atcgcgtccg tgccgggaac ctttccctgc ccgatcaccg tcaaggtcga
      541 cggcgaagcc tgcccggtgt tcgccggccg cctgatcaag ggcgtccgga acggccagtc
      601 gcccaagtgg cttcaggacc gcctgctggc catcggcctg cgcccgatca gcgccctggt
      661 cgatatcacc aacctgatca cctatgaccg cgcccggccg ctgcacgtct atgacgtcgc
      721 caagctgtcg gggaccgaga tctcgacccg cctgggccgc cacggccagc atggcgacga
      781 gcacctgatc gcgctggacg gcaagaccta tgagctgacg cccgacatgt gcgtgatcgc
      841 cgacgcctcg ggcgaacgcc cgatcggcct gggcggcgtg atgggcggcg aaagcaccgg
      901 ctgctcggaa gagacggtcg atgtcttcgt cgagagcgcc tggttcgagc cgatccgcat
      961 cgcccagacc ggccgcacca ccggcatcaa cagcgacgcc cagtatcgct tcgcccgcac
     1021 ggtcgacacc cactcgctgg tcccaggcat tgaactggcc acccggttga tcctggacct
     1081 ctgtggcggc gagccgtcgg agatcgtggt ggcggggcaa gccccggccg cgccggccgg
     1141 cttcgccttc gagcccgact atgtcgagca gctgtcgggt ctggcggtga cgcccgagca
     1201 gacgcgccag atcctgtccg acctgggctt cgacatcgtt ctgggttcgc cctggaccgt
     1261 gacgccgccc accttccgcc gtgacgtgga aggcaaggct gatctggtcg aggaagtcgc
     1321 ccgcatcgcc ggctatggcg ccctgccctc gacggccctg cccgaggttc cccgcgccgt
     1381 cggcggcgtc ctgaccgcca aacaggcgcg cggtcgcctg gcccgtcgcg ccctcgcggc
     1441 cgccggctat gccgaggccc taacctggag cttcaccaac cagaagatcg cgacgctgtt
     1501 tggcggcggc gcggccgagc tggtgctggc caatccgatc gcctcggacc tggactgcat
     1561 gcgcccgtcg ctgctgccca atctgatcga ggccgccggc cgcaacgccc gcaagggttt
     1621 cccggacgca gccctgttcg agatcggtcc caccttccac ggcgaccgac cggcagacca
     1681 gcgcaccgtg atcgcggcga tcatcgcccc gcacgcccca cgcggctggg ccaagacggc
     1741 gcaggacgac ctgttcaccg tcaaggccga cctgctggcc ctgctcgaag agctgggcgc
     1801 gccggtggat aaactgcaga cggcccaagg ctcggcctcg tcctggtggc atccgggccg
     1861 ctcggcgcgc ctgcagctgg gccccaaggc ggtgatggca gagttcggcg aactgcaccc
     1921 cgccgtgctg aaggcgctgg acgtggccgg cccggtctat ggcttcgaga tcaccctgga
     1981 agccatcccc gaacccaaga agaaggccca caagtcaaag gccgccttct cgccctcgcc
     2041 gctgatgccc ctgacccgcg acttcgcctt cctggtcgag acggccaaac cggccggcga
     2101 tctggtcaag gccgccctcg gcgccgacaa ggcgctgatc acggccgtgc gggtgttcga
     2161 cgtctatgac ggcgccggcg tgcccgacgg cttcaagtcg gtggcgctgg aggtggtggt
     2221 gcagccgcgt gaggcgaccc tgaccgacgc cgatatcgag gcgttgtcgg gccggatc
//
LOCUS       DAOMEO010000514         2278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5032_length_2278_cov_1.818264, whole genome shotgun sequence.
ACCESSION   DAOMEO010000514 DAOMEO010000000
VERSION     DAOMEO010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_2278_cov_1.818264"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..489
                     /locus_tag="PLH31_11520"
     CDS             61..489
                     /locus_tag="PLH31_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQR89862.1"
                     /translation="MAKGPNAFRTISEAAEELGVPQHVLRFWETKFSFIRPMKRAGGR
                     RFYRPQDIAVLNGVRVLLYGEGLTIKGVQKLHRDQGLSHVIAAGLGSNGSAMLDAGAV
                     GAIEAASGHELAEAARDRLAFVLDNLEVVKARLDGLLKRQ"
     gene            562..638
                     /locus_tag="PLH31_11525"
     tRNA            562..638
                     /locus_tag="PLH31_11525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:596..598,aa:Pro,seq:ggg)
     gene            complement(709..954)
                     /locus_tag="PLH31_11530"
     CDS             complement(709..954)
                     /locus_tag="PLH31_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HQR89863.1"
                     /translation="MSPSPTAARLLIPARLRKLIGGLGIMVFLTAYVVIATTLYDYLP
                     DNRAVHLLYFAIVGLAWGLPLMPLMSWMGKADKPVIR"
     gene            complement(951..1202)
                     /locus_tag="PLH31_11535"
     CDS             complement(951..1202)
                     /locus_tag="PLH31_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQR89864.1"
                     /translation="MSGVGVFATALIGIFAGWFADLVLGRRHSLFFKLLIGVIGAFIG
                     VFIAQRLELPLAGFWGGLIVASIGATLFLAVLGLIRRPA"
     gene            1349..>2278
                     /locus_tag="PLH31_11540"
     CDS             1349..>2278
                     /locus_tag="PLH31_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQR89865.1"
                     /translation="MTSFLRSDRSRPVAIWLFIVAALVFAMVVVGGATRLTDSGLSIT
                     EWKPIMGALPPMSEAAWQADFELYKQIPQYKLVNAGMTLEAFKTIFWWEWGHRLLGRV
                     VGAAFAIPFAFFLIRRMIPRRLIWRCAVMLMLGGLQGLIGWWMVSSGLSERVSVAPER
                     LMTHLGLALALFVMLIWTALDAWAGSPRIEERSPWRGWALALLGAIYFQSLLGALVAG
                     NDAGLVYNDWPLMNGALLPDQYFGKSLWGTLAHSHGAVQLHHRLGAYVLFAAGIVVGV
                     LALRSRHLPLEARMAGIAVAIIVTLQAALGVWTL"
BASE COUNT          379 a          706 c          774 g          419 t
ORIGIN      
        1 cgcctcgcag gtcatgaagg cgcgcatcga ccgcgccctg ggcggctagg cacacacgcg
       61 gtggcgaagg ggcccaacgc cttccgcacg atctccgaag ccgctgaaga gctgggcgtg
      121 ccgcagcatg tcctgcggtt ctgggaaacc aagttctcct tcatacggcc catgaagcgg
      181 gccggcggcc ggcgattcta tcgcccgcag gacatcgccg tcctgaacgg cgtgcgcgtg
      241 ctgctgtacg gcgaaggcct gaccatcaag ggcgttcaga agctgcatcg cgaccagggc
      301 ttgtctcatg tcatcgcggc gggtctgggc tccaatggct cggccatgct ggatgctggc
      361 gccgtcggcg cgatcgaggc tgcttcaggt catgaactgg cggaggcggc ccgcgatcgg
      421 ctggccttcg tcctggacaa tctcgaggtc gtcaaggcgc gtctggacgg gcttctgaag
      481 cgccagtagg gcgcttggaa attcccctgg aataccggtt gcggaggtca gggccggcgc
      541 ctataagagc gcctcttccg tcggagcgtg gcgcagcctg gtagcgcact tgactggggg
      601 tcaaggggtc gcaggttcaa atcctgtcgc tccgaccatt tccgcaaggg aataaacgga
      661 agaacggaaa ggccgcccta cggggcggcc tttttcggtt tctccctgct agcggatgac
      721 gggcttgtcg gccttgccca tccacgacat cagcggcatc agcggcaggc cccaggccag
      781 gccgacgatg gcgaagtaca gcagatgcac cgcgcggttg tcgggcagat agtcatagag
      841 cgtggtggcg atcaccacat aggcggtcag gaagaccatg atgcccaggc ccccgatcag
      901 cttgcgcagc cgggcgggaa tcaacaggcg ggccgcagtc ggggaagggc tcaagcgggt
      961 ctccggatca ggccaagcac ggccaggaaa agcgtggcgc cgatactggc gacgatcagg
     1021 ccgccccaaa agccggctag cggcaattcc aggcgctggg cgatgaagac gccgataaag
     1081 gctccgatca cgccaatcaa cagcttgaag aacagggagt gacggcgccc cagcacaagg
     1141 tcggcgaacc agccggcaaa gatgccgatc agcgccgtgg cgaacactcc aacaccactc
     1201 atcaggtcct cccgccgagc agattgtcgg cgccggcatc ttcgcacgaa aatcccttaa
     1261 gcgtcatagg cgatggacgg cggcgcgcgc cgggagtacg ggtcggccgg tcgcatttcg
     1321 gcccagaatt gtcgctttgt ccggtagcat gacgtctttc ctgcgctctg accgttcccg
     1381 tcccgtcgcc atctggctgt tcatcgtggc ggccctggtg tttgccatgg tcgtcgtcgg
     1441 cggggctaca cgcctgacgg actccggtct ttcgatcacg gagtggaagc ccattatggg
     1501 cgccctgccg cccatgtctg aggccgcctg gcaggccgat ttcgagctct acaagcagat
     1561 cccgcaatac aagctggtca atgccggcat gacgcttgag gccttcaaga cgatcttctg
     1621 gtgggagtgg ggccatcggt tacttggccg tgtcgtgggc gcagcgttcg cgattccatt
     1681 cgcgttcttc ctgatccgcc gaatgatccc gcgccgcctg atctggcgtt gtgcggtgat
     1741 gctgatgctg ggcggactgc agggcctgat cggttggtgg atggtgtcca gcggcctgtc
     1801 cgagcgggtg tctgtggcgc ccgagcggct gatgacccat ctgggcctcg cactggcgct
     1861 gttcgtcatg ctgatctgga cggcgctgga cgcctgggcc ggctcgccgc gcatcgaaga
     1921 gcgctcgccc tggcgcggct gggccctggc cctgctgggc gccatctact tccaaagcct
     1981 gctgggcgcg ctcgtcgcag ggaacgatgc ggggctggtc tataacgact ggcctctgat
     2041 gaacggcgcg ctgcttcctg accagtattt cgggaaaagc ctttggggca cgctggcgca
     2101 cagtcacggc gcggtgcaat tgcaccacag gctcggcgcc tatgttctgt tcgcagcggg
     2161 gatcgtggtg ggggtgctgg ccttgcgatc gcgtcatctg ccgcttgagg cccggatggc
     2221 cggcatagcg gtcgcgatca tcgtcaccct tcaggcggcg ctgggcgtct ggaccctg
//
LOCUS       DAOMEO010000515         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5034_length_2277_cov_2.245725, whole genome shotgun sequence.
ACCESSION   DAOMEO010000515 DAOMEO010000000
VERSION     DAOMEO010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_2277_cov_2.245725"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>541
                     /locus_tag="PLH31_11545"
     CDS             23..>541
                     /locus_tag="PLH31_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HQR89866.1"
                     /translation="MLIGLGAASVSGLAGALAWGLTAKSGRSATPEVYAVGGQPPERL
                     SALPKDYAGRAKDVPPLGPPLPGDLGRPILSAQQAGVEVSPPAIAPIAPERATPDPRV
                     QARDAARASGLFLASAARSPGPASRADEAVWTVAPDASSVGDPRLVSAERLQAPVSPY
                     LLQAGAMIPAVMI"
     assembly_gap    542..551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <552..1027
                     /locus_tag="PLH31_11550"
     CDS             <552..1027
                     /locus_tag="PLH31_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HQR89867.1"
                     /translation="ETVRDSVTGEHVLIPQGSRLVGDYVSDIAHGQSRVALVWTRLSL
                     PDGRAIVLDKLPASDLEGYSGLQDRVDRHWRATFGAALLSTILAVGSETGADDGDSDL
                     ARAIRRSGAQSLNQVGQQAVGQGLSLTPTLSIRPGAPVQVMVTRDLVLEPLMARR"
     gene            1030..1245
                     /locus_tag="PLH31_11555"
     CDS             1030..1245
                     /locus_tag="PLH31_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HQR89868.1"
                     /translation="MATLRLSTLEDDTPVKVSLEVPGPVYRNLTAYAEQMGQQAGKAA
                     EPAKLIVPMVELFMKSDRAFKPKRERG"
     gene            complement(1242..1676)
                     /locus_tag="PLH31_11560"
     CDS             complement(1242..1676)
                     /locus_tag="PLH31_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR89869.1"
                     /translation="MATDQQPHPVDRHVGARLRLARQLLHVSQRAVADALGVTFQQMQ
                     KYELGSNRISASKLYAAADALGRPVGWFFEGLAPIDTETGAPPNHASNLASVNDFLAS
                     RDGAELALRLSRIKPAHRRQILALARALDIDAADDKAEADSA"
     assembly_gap    1819..1828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1873..2229
                     /locus_tag="PLH31_11565"
     CDS             1873..2229
                     /locus_tag="PLH31_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89870.1"
                     /translation="MTPDAFHALVLQLAAVRAKPVLDTIQFQVAARTFATLGWPATGW
                     AVIKLSEADQRRALRLDPAFRAEAGPRGRRGVTLVSLEAVRQDHLAEVLACGWRQASG
                     TRKGGALAGQDMARSA"
BASE COUNT          337 a          728 c          823 g          369 t
ORIGIN      
        1 ggtgacgcga ctgtcacgca aggtgctgat cggcctgggc gcggcctccg tgtccggcct
       61 ggccggcgct ctggcctggg ggctgacggc caagtccggg cggtcggcaa cgccggaggt
      121 ctatgcggtc ggcggccagc cccccgaacg gctgtcggcc ttgcccaagg actatgccgg
      181 acgagccaag gacgtgccgc cgctaggccc gcctctgccg ggggatctgg gccggccgat
      241 cctcagcgcg cagcaggccg gcgtcgaggt ctcgccgccg gcgattgcgc cgatcgcgcc
      301 ggagcgtgcg acacccgacc cgcgggttca ggcgcgagac gccgcgcgcg ccagcggact
      361 cttcctcgcc agcgccgcgc gatctcccgg cccggcgtcc agggcggacg aggcggtctg
      421 gacggttgct cctgacgctt catcggtggg tgacccgcgt ctggttagcg ccgagcggct
      481 gcaggccccg gtctcgccct atctcctgca ggccggagca atgatcccgg cggtgatgat
      541 cnnnnnnnnn nccgaaaccg tgcgtgacag cgtcacgggc gagcatgtgt tgatcccgca
      601 aggttcgcgc ctggtcggcg actatgtcag cgacattgct cacggccaga gccgcgtcgc
      661 cctggtctgg acgcgcctgt ccttgcccga tggtcgcgcc attgtgctcg ataagctccc
      721 cgcgagcgat ctcgagggct attcgggact gcaagatcgt gtcgatcggc actggcgagc
      781 gacgttcggc gcggccctgc tcagcaccat cctggcggtc ggctccgaga cgggggccga
      841 cgacggcgac agcgatctag cccgcgccat acggcgctcg ggcgcccagt cgctcaatca
      901 ggtcggtcag caggctgtgg ggcaggggct gtcgctgacc ccgaccctga gcatccgacc
      961 cggcgcgccg gtgcaggtga tggtcactcg cgaccttgtt cttgaaccct tgatggcgag
     1021 gagatgatca tggccaccct caggctctcg acccttgaag acgacacacc ggtgaaggtc
     1081 agcctcgaag ttccggggcc ggtctatcgc aacctgacgg cctatgccga gcagatgggt
     1141 cagcaggccg gcaaggccgc cgagccagcc aagttgatcg tgccgatggt cgagctgttc
     1201 atgaaatcgg accgcgcctt caagcccaag cgtgagcggg gctaagcgct gtcggcctcg
     1261 gccttgtcgt cggcggcgtc gatgtccagc gcgcgggcca aggccagaat ctggcggcgg
     1321 tgggccggct tgatgcgcga cagccgtaag gccagttccg cgccgtcgcg agaggccagg
     1381 aagtcgttca cgctggcgag gttgctcgca tgattgggcg gggcgccggt ctcggtgtcg
     1441 atcggcgcaa gaccttcgaa gaaccagccg acggggcggc ccagggcgtc ggcggccgca
     1501 tagagcttgg aggcgctgat gcggttggag cccagctcgt atttctgcat ctgctggaag
     1561 gtgacaccga gcgcgtcggc caccgcgcgc tggctgacat gcagcagttg gcgggccagg
     1621 cgcagacgcg cgccgacatg gcgatcgacg ggatgaggct gttgatcagt ggccaaggat
     1681 caggtccgta tgagggtctc aagtctcgcg gcgcagcggg gcgtttcaac ccggcggagc
     1741 gcgacggctt ttacacgtta tgagaaggtc gcaaacggca aggttgaggg gcgcgaagat
     1801 ttgcgctccg gtcgccggnn nnnnnnnncg tggtcaccat tagggtcttg tgccccgctc
     1861 ggatgagagc cgatgacgcc cgacgctttc cacgccctgg tcctgcaact ggccgccgtc
     1921 cgcgccaagc cggttctgga caccattcag ttccaggtcg cggcgcgtac cttcgcgacc
     1981 ttgggctggc ccgccaccgg ctgggcggtc atcaaactct cggaagccga tcagcggcgc
     2041 gcgctgaggc ttgacccagc atttcgcgcc gaggccgggc cgcgtgggcg gcgaggtgtg
     2101 accctcgtta gcctcgaagc ggtgcgccaa gatcatcttg cggaggttct cgcctgcggt
     2161 tggcgtcagg ctagcggaac gcgaaagggc ggggctctgg ccgggcagga catggcgcgc
     2221 agcgcttagg atcttagtgg ggggagtggg cggcccggca agtcggggag cgatggc
//
LOCUS       DAOMEO010000516         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5035_length_2276_cov_2.410626, whole genome shotgun sequence.
ACCESSION   DAOMEO010000516 DAOMEO010000000
VERSION     DAOMEO010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_2276_cov_2.410626"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1390)
                     /locus_tag="PLH31_11570"
     CDS             complement(29..1390)
                     /locus_tag="PLH31_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR89871.1"
                     /translation="MRRSIWTSFRTRLIAGAVIWITAGLAVSGWVLAELFRAHVTEQF
                     DEELHGHAAELAVLAAVDPSGQPYLLRRLSDPRFLPQGSGFYWRLQAPGGRSVSSPSL
                     GNGRLHLAETPAPVGVERHAFADGPTGLVRIVETSAKPAGATAPLRIAIGVEQRLLDQ
                     VLARFNWTLAASLLFIALGLSGAAMIQVWFGLRPLCRVRSALAAVQTGQAHRLPDDLP
                     NEVAPLAADLNNLLEANLDMLRRARTQAGNLGHALKTPLAILVDEAQRLRSGGNAAAA
                     EVIDQQCERMQRQIDYQLARARAAALRSAPGVATSIEDALKPVLEAMRRLHGRRGVAF
                     EAAVAPGLVAALDAQDLAEILANLMDNGGKWARGRVEVIARPPHGGHILVTVDDDGPG
                     LAPEAYELVFGLGERLDEQMPGHGMGLAIVKDLVVLYGGKIWMDASPLGGLRVNLQLP
                     AIV"
     gene            complement(1387..2037)
                     /locus_tag="PLH31_11575"
     CDS             complement(1387..2037)
                     /locus_tag="PLH31_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR89872.1"
                     /translation="MRILLIEDDQDLARRLADALGEAGFAVDCAADGETGCLMGETND
                     FDAVVLDLGLPRLPGIEVLKRRRAGRDVPVLILTARDAWTDRVEGLNAGADDYLGKPF
                     QSAEVVARLRALTRRSAGRASPLLRRGDIVLDPAASAITQNGELIELTARELRVLTYL
                     MHRAGRIVSQAELVDHVYALDETRESNTIEVYIGRLRKKLGKDVIRTVRGLGYRMT"
BASE COUNT          384 a          771 c          763 g          358 t
ORIGIN      
        1 gagaggggac gctgaccgcg acaccgcgtc aaacgattgc cggcaactga aggttcacgc
       61 gaagcccgcc caggggcgag gcgtccatcc agatctttcc cccgtagagg acgaccagat
      121 ccttgacgat ggccagaccc atgccgtggc ccggcatctg ctcatccagt cgctcgccca
      181 ggccaaagac caattcgtag gcctctggag ccagacctgg gccatcgtcg tccaccgtca
      241 ccaggatgtg tcccccgtgg ggtgggcgtg cgatgacctc aacgcggccg cgggcccatt
      301 tcccgccgtt gtccatcagg tttgccagga tctcggcgag gtcttgggcg tccagcgccg
      361 caacaagacc aggcgcgacc gcggcttcaa acgcgacgcc ccggcgaccg tgcagtcttc
      421 gcatggcttc caggacaggc ttcagcgcgt cttcgatcga cgtggccacg cccggtgcgc
      481 ttcgcaacgc cgcggcgcgg gcgcgagcca gctgatagtc gatctgccgt tgcatgcgct
      541 cgcactgctg atcgatgacc tccgcggccg cggcgttccc gcctgagcga agtcgctggg
      601 cttcgtccac tagaatggcc agaggcgtct tcagggcatg gccaaggttg ccggcctgcg
      661 tgcgggcccg gcgcagcatg tccagattgg cctcaagcaa gttgttgagg tcagcggcca
      721 ggggggcgac ctcgttgggc agatcatccg ggagccgatg cgcctgaccc gtctggacgg
      781 ccgcgagcgc gcttcgcacc cggcagagcg gacgcagacc gaaccagacc tggatcatgg
      841 ccgcgccgct caggcccagg gcgatgaaca gcaacgacgc cgccagcgtc cagttgaacc
      901 gcgccagcac ctgatccagc agacgctgct ccacgccaat ggcgatgcgc aagggcgccg
      961 tcgcgccggc cggcttcgcg ctggtctcga caatcctgac cagaccggtg gggccatcgg
     1021 cgaacgcgtg gcgctcgaca cccaccgggg ccggggtctc cgccaggtgc agcctgccgt
     1081 ttcccagcga cggcgagctg accgaacgtc cgccgggggc ctgcagccgc cagtagaatc
     1141 cggacccctg tggcagaaag cgcggatcgc taagtcgccg gagcaggtag ggctgtccgg
     1201 acgggtcgac ggcggcgagg acggcaagtt cggcggcgtg gccatggagt tcctcgtcga
     1261 actgctcggt gacatgggcc cgaaacaact cagccagcac ccagccgctc acggcaaggc
     1321 cggcggtgat ccagataacg gcgcctgcaa tcaggcgagt gcggaagctg gtccagatcg
     1381 aacgcctcat gtcattcggt atcccaggcc gcgcacggtg cggatgacgt ccttgccaag
     1441 cttctttctc aggcggccga tatagacctc gatcgtgttg gactcgcggg tctcatccag
     1501 cgcgtagaca tgatccacca gctcagcctg ggacacgatc cgacccgcgc gatgcatcag
     1561 gtaggtgagg acgcgcagtt cacgcgcggt cagttcaatc aactcgccgt tctgggttat
     1621 ggcgctggcg gcggggtcca gaacgatgtc gccccgacgc agaagtggag aggcgcgtcc
     1681 tgccgaacgg cgcgtcaggg ccctgagacg ggcgacaacc tcggcgctct ggaacggttt
     1741 gccaagatag tcatcggcgc cagcgttcag cccctcgacg cgatcggtcc aggcgtcgcg
     1801 cgcggtgagg atgagcaccg ggacgtctct gcccgcacgc cgccgcttta ggacctcgat
     1861 ccccggcagg cgcggcaggc cgagatccag cacgacggcg tcgaaatcat tggtctcgcc
     1921 catcaggcag ccggtctcgc catcggccgc gcagtcaacc gcgaagccgg cttcgcccag
     1981 cgcgtcggcc agccgtcggg ccagatcctg gtcgtcctcg atgagcagaa tgcgaatggt
     2041 cgcgtctccc ttcgccaagg gctctagcac cggcgcctga agccagcctg aacgcgggcg
     2101 cggggtttgt caggttcatt tcagcttcgc ttcaggcggg cttcagggcc agggcgcatt
     2161 tgagggtgcg gtcgcggatc gccgcgcgag gatgaccatg accacacgaa actgttcgct
     2221 ggccgccggc gtggcgctgg ccgccgtatg gctttgcggg acgccgtcct tcgccg
//
LOCUS       DAOMEO010000517         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5045_length_2274_cov_2.278954, whole genome shotgun sequence.
ACCESSION   DAOMEO010000517 DAOMEO010000000
VERSION     DAOMEO010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5045_length_2274_cov_2.278954"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..705
                     /locus_tag="PLH31_11580"
     CDS             193..705
                     /locus_tag="PLH31_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HQR89873.1"
                     /translation="MRIKALLALTAATLSLSACATADRYDAAGDVHALLTAIRDRDRA
                     AFDARVDRRALKYEIEARLLDEARKRKADNSLMAIAAALAGPAADIAGEALIRPETFR
                     AAANYYGYTPDRPLPGRIAIAGALRPAGDGRVCAARKDGPCLLTFTRDGSVWRLSGFD
                     PQAANLHFKR"
     gene            702..1250
                     /locus_tag="PLH31_11585"
     CDS             702..1250
                     /locus_tag="PLH31_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89874.1"
                     /translation="MRLALPIVLAASALATTAQAEKRMFSYDPISPDARRLTGAGLTV
                     LFDQGLMSAKPVKVLATGVPAQGLLIKGKDRDLGPKGLAGLEGVDADAALYEIDPKAE
                     QGRVYIRAFCPGATRLWLSFSRIAVRRDLRIQALGDDPKTPGQARVCGTLDFSWRGEW
                     KLPDRHRPDPMEDWTDNLNGVR"
     gene            1413..2201
                     /locus_tag="PLH31_11590"
     CDS             1413..2201
                     /locus_tag="PLH31_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR89875.1"
                     /translation="MSSALDGFTRFEFDDGRWTREVWRIGTGPAVIVIHEIPGLHPGV
                     LAFARDVAAAGMTVFCPSLFGQPGKPVSNGYALGVIAKTLCVRREFTAWATDRSSPIV
                     DWLRALARQAHKECGGKGVGAVGMCFTGGFALAMMTEPSVVAPVLSQPSLPLGKAGRA
                     SLGCSAVEIACARTRFETEDLSLLALRFSSDKLVPDARFETLKREFGDRVETVELKDS
                     DAAPNPAMAPHSVLTLHLDRSDPLGATMQTRDKVIAFFKARTEA"
BASE COUNT          343 a          794 c          774 g          363 t
ORIGIN      
        1 agcttgtcgc gcgctgaaga cctcacgctc tacgacgccg cctatctaga actggcgatc
       61 cggcttggag ccacactcgc gtcatttgac agagagctga ttgccgccgc gcgccgcaac
      121 ggtgtcaacg ttgtgaccgc ctaaaagtcg ccggactgga attcgcccaa ttccggcgct
      181 acgccttccg ctatgcgtat caaagccctc ctcgccctga ctgccgcgac gctctcgctg
      241 agcgcctgcg ccaccgccga ccgctatgat gcggccggcg atgtccatgc cctgctgacg
      301 gcgatccgcg accgcgaccg ggccgctttc gacgcccgcg tcgatcgtcg ggcgctgaaa
      361 tacgagattg aggcgcggct gctggatgag gctcgcaagc gcaaggccga caacagcctg
      421 atggccatcg ccgccgccct ggccggtccc gccgccgata tcgcgggcga ggcgctgatc
      481 cggcccgaga ccttccgcgc cgccgccaac tattacggct acacgcccga ccgcccgctg
      541 ccgggccgga tcgctatcgc cggcgccctg cgtccagccg gcgacggccg ggtttgcgcg
      601 gccaggaagg acggtccctg cctgctgacc tttacccgcg acggctcggt ctggcggctg
      661 agcggttttg atccgcaggc ggcgaacctg cacttcaagc gatgagactg gcgttaccga
      721 tcgttctggc ggcctccgct ctggccacca ccgcccaggc cgaaaagcgg atgttttcct
      781 acgacccgat ctcgcccgac gccaggcggc tgaccggcgc gggtctgacc gtgctgttcg
      841 accagggcct gatgagcgcc aaacccgtca aggtgctggc caccggtgtt ccggcccaag
      901 gcctgctgat caagggcaag gaccgcgacc tgggccccaa agggctggcg ggtctggagg
      961 gtgtggacgc cgacgcggcg ctgtacgaga tcgaccccaa ggctgagcag ggccgggtct
     1021 atatccgcgc cttttgtccg ggcgccacgc gtctgtggct gagcttcagc cggatcgccg
     1081 tgcgccgcga cctgcgcatc caggcgctgg gagatgaccc caagacgccg ggtcaggcgc
     1141 gggtatgcgg cacgctggac ttctcctggc gcggcgagtg gaagctgccg gaccgccacc
     1201 gtcccgatcc gatggaagac tggaccgaca acctgaacgg tgttcgctag ggccgctaaa
     1261 gctgtcatcc cggccaagcg cagcggagag ccgggaccgc aaaagctcgg cgtttccggc
     1321 ggtcccggct cggcgcgctt cgcgcttggc cgggatgaca acgagaagga tgtcggccaa
     1381 gctcccgccc aacgacacgg gggtgggaaa caatgagcag cgcgctggac ggcttcacgc
     1441 ggttcgagtt cgacgacggg cgctggaccc gcgaggtctg gcggatcggg acgggtcccg
     1501 ccgtcatcgt catccacgaa atccccggcc tgcatcccgg cgtcctggcc tttgcccgcg
     1561 acgtggcagc ggccggcatg accgtcttct gccccagcct gtttggccag cccggcaagc
     1621 cggtcagcaa cggctacgcc ctgggcgtca tcgccaagac cctgtgcgtg cgccgtgagt
     1681 tcaccgcctg ggccaccgac cgctccagcc ccatcgtcga ctggctgcgg gccctggccc
     1741 ggcaggcgca caaggagtgc ggcggcaagg gcgtgggcgc agtgggcatg tgcttcaccg
     1801 gcggctttgc cctggcgatg atgaccgagc cgtcggtggt ggcgccggtt ctgtcgcagc
     1861 cgtccttgcc gctgggcaag gcgggccggg cgtctctggg ctgctccgcc gtcgagatcg
     1921 cctgcgcccg cacgcggttc gagaccgagg acctgtcgct gctggccctg cggttctcgt
     1981 ccgacaagct ggtgcccgac gcgcggttcg agacgttgaa gcgcgagttt ggcgaccgcg
     2041 tggagacggt ggaactcaag gactctgacg ccgccccaaa cccggccatg gccccgcact
     2101 cggtcctgac cctgcacctg gaccgttccg acccgctggg cgcgaccatg cagacgcgcg
     2161 acaaggtgat cgcgttcttc aaggcgcgga cggaggcgta agctggatgc tccccctctg
     2221 ggggagctgt cgcggagcga ctgagggggc taatgcggct tatgccgtgc tggc
//
LOCUS       DAOMEO010000518         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5054_length_2269_cov_2.659892, whole genome shotgun sequence.
ACCESSION   DAOMEO010000518 DAOMEO010000000
VERSION     DAOMEO010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_2269_cov_2.659892"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="tatA"
                     /locus_tag="PLH31_11595"
     CDS             <1..207
                     /gene="tatA"
                     /locus_tag="PLH31_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQR89876.1"
                     /translation="IHWVIVIAVIALLFGGRGKLSGIMGDAAKGIRAFKDGLKDDTSS
                     EVADNKATGALPRTKAEEEDLRKS"
     gene            302..907
                     /gene="tatB"
                     /locus_tag="PLH31_11600"
     CDS             302..907
                     /gene="tatB"
                     /locus_tag="PLH31_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HQR89877.1"
                     /translation="MLPDIGGAELLVIAAVALIVVGPKDLPLLLRKLGQFVGKMRGMA
                     SEFRASFDEMARQSELDDLRREVEAMRSGQYTAPLREAADQAKDVGVDQVFADIDASL
                     NSGAVQVHPYAGYQPNDASSEPSILPPETVVEPVAAKPRTPRKPRAAKAPMMVEPAPA
                     KAPARKRAAKTEIAVEAPKAARAPRKKAVKAGGSTASDIIS"
     gene            904..1803
                     /gene="tatC"
                     /locus_tag="PLH31_11605"
     CDS             904..1803
                     /gene="tatC"
                     /locus_tag="PLH31_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQR89878.1"
                     /translation="MTDALGHEPEDEIEASRAPLMEHLVELRMRLIICVLALFVGFGV
                     CFAFADQIFLFLVKPFTVAQQLLAAQHSGAKHGPFDLLLALVGLKDIPMGQTKALDLV
                     YTAALEFFFTKIKLAGFGAVVLTFPVLAWQLYRFVAPGLYKNERGAFLPFLLASPFMF
                     FLGAALVYFIMLPFVLWFSLSQQVQTAGISIQLLPKVSDYLTLVTSLLLAFGLCFQLP
                     VVVSLLGLAGIISSKLLREGRRYAIVGVFIAAAILTPPDPISQLTLALPMCLLYEIGI
                     WCVWLIERSKAKAEEKSNAVVPV"
     gene            complement(1902..>2269)
                     /locus_tag="PLH31_11610"
     CDS             complement(1902..>2269)
                     /locus_tag="PLH31_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89879.1"
                     /translation="INNEIKAAAQTVCAKEKAAFVAACLESTTYDANRQLASILKGRT
                     GATKVSARREAVSVVRISLKGKSAAEINAEITTAAKAVCKASNDFTNRYEFQACVGGT
                     VRSAKAQLQAMSVRPLTDA"
BASE COUNT          345 a          751 c          747 g          426 t
ORIGIN      
        1 attcactggg tgatcgtgat cgcggtcatt gccctgctgt tcggtggccg cggcaagctg
       61 tcgggaatca tgggcgacgc cgccaagggc attcgcgcgt tcaaggacgg cctgaaggac
      121 gacaccagca gcgaagtcgc cgacaacaag gccaccggcg ccctgccgcg caccaaggcc
      181 gaagaagaag acctgcgtaa gtcttgagga tcctccgggc gcgcgggcgg agcccgtcgc
      241 gcgcgtccca ttcatttcgc gacggcgggc tttaaggtcc gcgtcgctct aaggcgcgtc
      301 catgcttcct gatatcggcg gcgcagaact tctggttatc gctgcggtcg ccctgatcgt
      361 ggtgggtccg aaggatctcc ccttgctgct gcgcaagctt ggccagttcg tgggcaagat
      421 gcgcggcatg gcgtcggaat tccgcgccag cttcgacgaa atggcccgcc agtccgagct
      481 cgacgacctg cgtcgcgagg tcgaggccat gcgctcgggc caatataccg cgccgctccg
      541 cgaggccgcc gaccaggcca aggatgtggg cgtggaccag gtgttcgccg atatcgacgc
      601 cagcctcaac agcggcgcgg ttcaggttca cccctatgcc ggctatcaac ccaatgacgc
      661 ctcgagcgag ccgtcgatcc tgccgcccga aacggtggtc gagcccgtcg cggccaaacc
      721 tcgcacgccc cgcaagccgc gtgcggccaa ggcgccgatg atggtcgagc cggcccccgc
      781 caaggccccc gcccgcaagc gcgccgccaa gaccgagatc gccgtcgaag cgcccaaggc
      841 cgctcgcgcg ccgcgcaaga aggctgtcaa ggctggcggc tcgaccgcct cggatatcat
      901 ctcgtgacgg acgccctcgg acacgaaccc gaagacgaga tcgaggcctc gcgcgcgccc
      961 ttgatggagc atctggtcga gctgcgcatg cggctgatca tctgcgtgct ggcgctgttc
     1021 gtcggctttg gcgtgtgttt cgcgttcgcc gaccagatct tcctgttcct ggtcaagccg
     1081 ttcacggtgg cccagcagct gctggccgcc cagcattcgg gcgccaagca cggtccgttc
     1141 gacctgctgc tggccctggt gggcctgaaa gacatcccga tgggccagac caaggccctg
     1201 gatctggtct atacggcggc gctggagttc ttcttcacca agatcaagct ggccggtttc
     1261 ggcgcggtgg tcctgacctt cccggtcctg gcctggcagc tgtatcgctt cgtggcgccg
     1321 ggtctctaca agaacgagcg cggggcgttc ctgccgttcc tgctggcctc gcccttcatg
     1381 ttcttcctgg gcgcggcgct ggtctatttc atcatgctgc cgttcgtcct gtggttctcg
     1441 ctgagccagc aggtgcagac ggccggcatc tcgatccagc tgctgcccaa ggtcagcgac
     1501 tatctgaccc tggtgacctc gctgctgctg gcctttggcc tgtgcttcca gctgccggtc
     1561 gtggtgtcgc tgctgggcct ggccgggatc atttccagca agctgctgcg cgagggccgt
     1621 cgctacgcca tcgtcggcgt gtttatcgcc gccgccatcc tgacgccgcc ggacccgatc
     1681 agccagctga ccctggccct gccgatgtgc ctgctctacg aaatcggcat ctggtgcgtg
     1741 tggctgatcg agcgcagcaa ggccaaggcc gaagaaaaga gcaatgcggt cgtgccggtc
     1801 tgatcccacg atatcgcgga tagcaaaaag cctccgcccc ggaagggagc ggaggctttc
     1861 ctgctttcgg gcggcccctc agggccatcc agccgtgagg cttaggcgtc ggtcagcgga
     1921 cgaacgctca tcgcctgcag ctgggccttg gccgagcgga cggtgccgcc gacgcaggcc
     1981 tggaattcgt agcggttggt gaagtcgttc gaggccttgc acacggcctt ggcggcggtg
     2041 gtgatctcgg cgttgatctc ggcagccgac ttgcccttca gcgagatgcg gaccaccgaa
     2101 acggcttcgc ggcgggccga aaccttcgtg gcgccggtgc ggcccttcag gatcgaggcc
     2161 agctggcggt tggcgtcata ggtggtgctt tccaggcagg cggcgacgaa cgcagccttt
     2221 tccttggcgc agacggtctg ggctgcggcc ttgatctcat tgttgatct
//
LOCUS       DAOMEO010000519         2269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5055_length_2269_cov_2.252484, whole genome shotgun sequence.
ACCESSION   DAOMEO010000519 DAOMEO010000000
VERSION     DAOMEO010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_2269_cov_2.252484"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLH31_11615"
     CDS             <1..399
                     /locus_tag="PLH31_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89880.1"
                     /translation="SFLPAPFDNIGAVANYTYVDAEEALTGVSKTSYNATLYYETSRW
                     GVRGSLSHRARWYSGVNDSVMSASTRGFEGGTYIDAAAFFNIADGLQLSLDAINLTNQ
                     EDTQFWGQNRYLYNQNQSGATYMVGLSYKF"
     gene            465..1103
                     /locus_tag="PLH31_11620"
     CDS             465..1103
                     /locus_tag="PLH31_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQR89881.1"
                     /translation="MIVSHRHRFIFAAVPKTGTHAVRQALREQLGEEDVEQVGLFVDK
                     RFPWEELAAIRHGHLSLRQVRPYLGEEAFSGYFKFAFVRNPFDRFVSYCAFMLRGGEV
                     FQQQPREAMRHFLFAEPPEQHILFQPQASLLVDEDGQTLLTDGVGRVEDMQGSYEAIC
                     ARLGIPSRPLDRVNGSRHGDYRRYYDQALIDGVTRRYAQDLDLFGYTFEGLR"
     gene            1100..1729
                     /locus_tag="PLH31_11625"
     CDS             1100..1729
                     /locus_tag="PLH31_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HQR89882.1"
                     /translation="MSDAVFSPAANPRKTTGIRKLGAVEVSALQTAVAAIPETVWNAE
                     NAGKPNRFEVLDATRHIVFRFIDNPRDWRVSHDRAGWPAWRGLLEPVLAQAVRAYGYA
                     RGVFPRVMLARMPPGGVIHPHIDANPAAKWPHKIHVPLSTNPGVVSFFGGEEHHFPPG
                     EAVEVDNLGPHWVRNNGDTDRVHLIFEYYDADQPDPDWLEPFLGAGLAR"
     gene            1726..>2269
                     /locus_tag="PLH31_11630"
     CDS             1726..>2269
                     /locus_tag="PLH31_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR89883.1"
                     /translation="MTTHDRDTLLREAMALRAEQRPAEALNVLARLQALHPQFSRLHQ
                     ERGHCHILLGEGPAAIRALGEAVSLNPTLPASWDMLEQLHRMTGDTVQASEAGRRLAV
                     LKQLPPEIVAANSLLADGDLSPAEKGLRDYLRKDGDNVGAQRLLARIRQMQDAPDEAE
                     ALLKSVLEESQGFSTSSLNQF"
BASE COUNT          386 a          743 c          739 g          401 t
ORIGIN      
        1 tcgttcctgc cggcgccgtt cgacaacatc ggcgcggtgg ccaactatac ctatgtggac
       61 gccgaggagg cgctcaccgg cgtgtccaag accagctaca acgccacgct ctactatgaa
      121 accagccgct ggggcgtacg gggttcgctg agccatcgcg cgcgctggta cagtggcgtc
      181 aacgactccg tcatgagcgc cagcacgcgg ggcttcgagg gcggcaccta tatcgacgca
      241 gcggccttct tcaacattgc cgatggactg cagctgtcct tggacgcgat caacctgacc
      301 aaccaggagg atacgcagtt ctggggccag aaccgctatc tctataacca gaaccagagc
      361 ggcgcgacct acatggtcgg gctcagctac aagttctagg acgaccctat gtccagaccg
      421 cgccccctcc tcaggagggg gcgcttttcc gtgtgtcccg aaacatgatc gtttcccatc
      481 gccaccggtt catcttcgcg gccgtgccca agaccggcac gcatgccgtg cgtcaggccc
      541 tgcgtgaaca gctcggcgaa gaagacgttg agcaggtggg cctgttcgtc gacaagcgct
      601 tcccgtggga ggaactggcg gcgatccgac atggtcacct gtccctgcgg caggtgcgtc
      661 cctacctcgg cgaagaggcc ttcagcggct atttcaagtt tgccttcgtg cgcaatccgt
      721 tcgaccgctt tgtctcctac tgcgccttca tgctgcgcgg cggcgaggtc ttccagcagc
      781 agccgcgcga ggcgatgcgc cattttctgt tcgccgaacc gccggagcag cacatcctgt
      841 tccagcctca ggcctcgctg ctggtcgacg aggacggcca gaccctgttg accgacggcg
      901 tggggcgcgt cgaggacatg cagggctcgt atgaggcgat ctgcgcgcgt ctcggcattc
      961 cctcccgtcc gctggaccgg gtcaatggca gccgacatgg cgactatcgt cgctactacg
     1021 atcaggccct gatcgatggc gtcaccagac gctacgccca ggacctggat ctgttcggct
     1081 acacctttga aggcctcaga tgagcgacgc cgtcttcagc cccgccgcca atccgcgcaa
     1141 gactaccggc atccgcaagc tgggtgcggt cgaggtctcg gctctgcaga cggctgtggc
     1201 ggcgatcccc gagacggtct ggaacgccga gaacgccggc aagcccaacc gtttcgaggt
     1261 gctggacgcg acgcgccaca tcgtttttcg cttcatcgac aacccccgcg actggcgcgt
     1321 ctcgcacgat cgcgcgggct ggccggcgtg gcgcggcctt cttgagcctg tgttggccca
     1381 ggccgtccgc gcctacggct atgcgcgcgg cgtattcccg cgcgtgatgc tggcgagaat
     1441 gccgccgggc ggggtgatcc accctcatat cgacgccaac cccgcagcca agtggccgca
     1501 caagatccat gtgccactgt cgaccaatcc cggggtggtg tcgttcttcg ggggcgagga
     1561 gcaccatttc ccaccgggcg aagcggtgga ggtcgacaat ctgggcccgc actgggtgcg
     1621 caacaacggc gatactgacc gcgtgcatct gatcttcgaa tattacgatg ccgatcagcc
     1681 ggatccggac tggctggagc cattcctggg ggcgggcctg gcgcgatgac tacccatgat
     1741 cgcgacacgc tgttgcgtga ggcgatggcc ctgcgtgcgg agcaacggcc ggcggaggcg
     1801 ctgaacgtcc tggcgcgatt gcaggcgctg catccgcagt tcagccgcct gcatcaggag
     1861 cgcggtcact gccatatcct gttgggcgag gggccggcgg cgatccgggc cctgggcgag
     1921 gcggtgagcc tcaatccgac cctgccggcc agctgggaca tgctggaaca gcttcaccgt
     1981 atgacgggcg acaccgttca ggcgtccgag gcggggcggc ggctggccgt gctcaagcaa
     2041 ttgccgccgg agattgtggc ggccaacagc ctgctggccg atggcgacct gtcgccggcc
     2101 gagaaggggc ttcgggatta tctgcgcaag gacggcgaca atgtcggcgc ccagcgcctg
     2161 ctggcgcgga tacgccagat gcaggatgcg ccggacgagg ccgaggcctt gctgaagtcg
     2221 gtgcttgagg aaagccaagg cttttccacc agcagcctca atcagttcg
//
LOCUS       DAOMEO010000520         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5096_length_2246_cov_3.105431, whole genome shotgun sequence.
ACCESSION   DAOMEO010000520 DAOMEO010000000
VERSION     DAOMEO010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5096_length_2246_cov_3.105431"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLH31_11635"
     CDS             <1..436
                     /locus_tag="PLH31_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR89884.1"
                     /translation="VMAVVEMREKNQRAAAETVTNILEELRITHIRKSPAVALSGGER
                     RRVEIARALASEPSFMLLDEPFAGIDPLAIADIREVIGYLKKRGIGILITDHNVRETL
                     DIIDRASIIHAGEVLFEGSPKEIIEDPEVRRVYLGDHFAERV"
     gene            617..2110
                     /gene="rpoN"
                     /locus_tag="PLH31_11640"
     CDS             617..2110
                     /gene="rpoN"
                     /locus_tag="PLH31_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQR89885.1"
                     /translation="MALGHRLELRQGQGLVITPQLQQAIKLLQLSNIELDAFVEAELE
                     RNPLLQRDEGESEVVSDVEREPAASDLGVDIVAEGSANSEMDATPDDVSPGERASGDG
                     EQAQEAGGQIDWSRAGGGGSFEHDGDYEGALTAIPTLSEHLQGQLAQSGLSATEQLVA
                     NVLIDGVDEGGYLRLDLAETAERLGCAETLVERVLTVLQGFEPTGVCARDVRECLALQ
                     LKDQNRYDPAMAALLDNLDMLARRDMTGLRKACGVDDEDLREMIAEIRSLTPRPGAAY
                     ASETAETLVPDVHVREGLGGMWHVELNTDTLPRVLVDQRYHARVSKGARTDQEKTFVS
                     DCMATANWLVKSLDQRAKTILKVSSEIVRQQDGFLAFGVEHLRPLNLKTVADAIGMHE
                     STVSRVTSNKYIATPRGVFELKFFFTSAIQSSEGGEAHSAASVRHKIKGLVEAEKRET
                     DVHSDDAIVEILKAAGVDIARRTVAKYREAMRIPSSVERRRLMKEAC"
BASE COUNT          405 a          753 c          718 g          370 t
ORIGIN      
        1 tgtcatggcc gtggtcgaga tgcgcgagaa gaaccagcgc gcggccgccg agacggtcac
       61 caatatcctc gaggaactgc ggatcaccca tatccgcaag tcgccagctg tggccctgtc
      121 gggcggcgag cgccgccggg tggaaatcgc ccgggccctg gccagtgagc cgtccttcat
      181 gctgctggac gagcccttcg ccggtatcga tcccctggcc atcgccgata tccgcgaggt
      241 catcggctat ctgaagaagc gcggcatcgg catcctgatc accgaccaca atgtccgtga
      301 gaccctggat atcatcgatc gcgcgtcaat cattcacgcg ggcgaagtgc tgttcgaggg
      361 ctcgccgaag gaaatcatcg aggatccgga agtgcgccgc gtctatctgg gcgatcattt
      421 cgccgagcgc gtctaggaaa aatctgtttc tgcagaacga cttatcccgc gcagccctct
      481 ggcttcgcgg gattttcgtt gtttggcgac gggactgaag gtcgcaccgg cccggatatt
      541 gcaggcgttg gcgagccgtt aacctagttg gccttcaatg gggatcgaac attttcgatt
      601 tgcccaggag ggcgcattgg ctctcggcca tcgacttgag ctccggcaag gccaaggcct
      661 cgtcatcacg ccgcagctac agcaggcgat caagctgctg cagttgtcca atatcgaact
      721 cgacgccttc gtcgaggccg agcttgagcg caatccgctc ctgcaacgcg atgagggcga
      781 gagcgaagtc gtatccgacg tcgagcgcga gccggccgcc agcgatcttg gcgtcgacat
      841 cgtggccgaa ggctcggcca attccgaaat ggacgccaca cccgacgacg tctcgcccgg
      901 cgagcgcgct tcaggcgacg gcgaacaggc ccaggaggcc ggcggtcaga tcgactggtc
      961 ccgcgcgggc ggcggcggct ccttcgaaca cgacggcgac tatgaaggcg ccctgacggc
     1021 gatcccgacc ctgtccgagc acctgcaggg ccagctggcc cagtcgggcc tgtcggcgac
     1081 cgagcagctg gtggccaatg tcctgatcga cggtgtcgac gagggcggtt acctgcgcct
     1141 ggacctggcc gagaccgctg agcgcctggg ctgcgccgag acgctggtcg agcgggtcct
     1201 gaccgtgctc cagggcttcg agccgaccgg cgtctgcgcg cgcgatgttc gcgagtgcct
     1261 ggccctgcag ctgaaggacc agaaccgcta cgatccggcc atggccgccc tgctcgacaa
     1321 tctggacatg ctggcccgcc gcgacatgac cggcctgcgc aaggcgtgcg gcgtcgatga
     1381 cgaggatctg cgcgagatga tcgccgagat tcgctcgctc accccccgcc cgggcgcggc
     1441 ctatgccagc gagaccgccg agaccctggt gcccgacgtc catgtccgcg agggtctggg
     1501 cggcatgtgg cacgtggagc tcaataccga caccctgccc cgggtgctgg tcgaccagcg
     1561 ctatcatgcc cgggtttcca agggtgcgcg caccgaccag gaaaagacct tcgtctccga
     1621 ctgcatggcc accgccaact ggctggtgaa gagcctggac cagcgcgcca agaccattct
     1681 gaaggtctcc agcgagatcg tgcgccagca ggacggcttt ctggccttcg gcgtcgagca
     1741 cctgcggccg ctgaacctga agaccgtcgc cgacgccatc ggcatgcacg agagcacggt
     1801 cagccgggtc acctccaaca agtacatcgc caccccgcgc ggcgtcttcg agctgaagtt
     1861 cttcttcacc tcggccatcc agtcatcgga aggcggcgaa gcccactccg ccgccagcgt
     1921 gcgtcacaag atcaagggcc tggtcgaggc cgaaaagcgc gaaaccgacg tccactccga
     1981 cgacgccatc gtcgagatcc tcaaggccgc cggcgtggac atcgcccgcc ggaccgtcgc
     2041 caagtaccgc gaagccatgc gcatcccctc gtcggtggag cgccgccgcc tgatgaaaga
     2101 agcttgctga tcgggtcctg ttcatcgcgg tcaggcgaca ctccccgcga tatccggcgt
     2161 tacagtcgga ccagcgagga gtccgcatga cgatcagcag tctttccgac gtgatcgagg
     2221 ggtttggcga ggacgcccgc ccatcc
//
LOCUS       DAOMEO010000521         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5105_length_2244_cov_2.659205, whole genome shotgun sequence.
ACCESSION   DAOMEO010000521 DAOMEO010000000
VERSION     DAOMEO010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5105_length_2244_cov_2.659205"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..927
                     /locus_tag="PLH31_11645"
     CDS             22..927
                     /locus_tag="PLH31_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR89886.1"
                     /translation="MNLRDLRYLLAVVEHEHFGQAARACGVSQPTLSVQIRKLEELVG
                     VALFERTSKAVTPTVACQQLIGHARDAVTAAEAILATARNLRDPLAGRFRVGIIPTLA
                     PYLLPLVFAPLREALPHLEVEPWEDQTSALLERLRAHELDAALLATEVDGPDLISQPL
                     FDEPFLAALPPDHPLAGQAVVTESDLARDILVLADGHCLRDQTLAACGQSGALSGPLR
                     AASLPTLLNMVAAGYGTTLVPGLAAGAAQDAGIVLRPLAEQTGRTVRIVWRANFPGRP
                     AVEAVGEIIARRLRGFAQGAAAGMV"
     gene            complement(924..1400)
                     /locus_tag="PLH31_11650"
     CDS             complement(924..1400)
                     /locus_tag="PLH31_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR89887.1"
                     /translation="MRILLAVTAAIALLASPVLAQDLKVTVAGRDTVMLTPAEVKALP
                     RAKATITVKGQPVVYEGAVLHGALLKAGVVSGERLMGRYLNQIVVLRAADGFSSILSI
                     AETDPVYRANPVILADTVNGKPLDAREGPYRVVVDGDLRNSRSPRGVVSIEVKAGF"
     gene            complement(1432..2037)
                     /locus_tag="PLH31_11655"
     CDS             complement(1432..2037)
                     /locus_tag="PLH31_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR89888.1"
                     /translation="MTPDLLLAFVLFAFATAGTPGPNNMMLLASGANFGFRRTILHIL
                     GISVGLGVMVVAMGWGLSGVFEAFPILHEILKWVGATYMLWLAWKIATATGINDKGAG
                     GQPMTFLEAAGFQWLNPKAWAMALGAVTTYAPEGGGPTAVLLLAGTFMLVGAPCSAAW
                     AGFGQGLRQFLDRPAILRTFNVSMAVLLVASLYPLMTQLNR"
BASE COUNT          377 a          776 c          762 g          329 t
ORIGIN      
        1 gaggaatgat caggcgcacc gatgaatctt cgagaccttc gctacctgct ggccgtcgtc
       61 gagcatgaac atttcggtca ggcggctcga gcctgcggcg tcagccagcc gacgctgtcg
      121 gtccagatcc ggaagctgga ggagttggtc ggcgtcgccc tgttcgaacg caccagcaag
      181 gcggtaaccc cgacggtcgc ctgccagcag ctgatcggcc acgcccgcga tgcggtgacg
      241 gcggccgagg cgatcctggc cacggcgcgc aatctccgtg acccgctggc cggacgtttc
      301 cgggtgggga tcatcccgac cctggcgccc tatctgctgc ccctggtgtt cgcgccgctg
      361 cgcgaggccc tgccccacct tgaggtcgag ccctgggagg accagaccag cgccctgctg
      421 gaaaggctgc gcgcccacga actggacgcg gccctgctgg cgaccgaggt tgacggacca
      481 gacctgatca gccagcccct gttcgacgag ccgttcctgg cggccctgcc gccagaccat
      541 ccgctggccg gacaggcggt cgtcaccgag agcgatcttg cgcgggacat cctggtgctg
      601 gccgacggcc actgcctacg tgaccagacc ctggcggcct gcgggcaaag cggcgcgctg
      661 agcggaccgc tgcgcgccgc cagccttccg acgctgctga acatggtggc ggcgggctat
      721 ggcacaacgc tggtcccagg tctggcggcc ggggcagccc aggatgccgg gattgttcta
      781 cgccctctgg ccgagcaaac ggggcgaaca gtgcggattg tctggcgcgc caatttcccc
      841 ggccgcccgg ccgtggaagc tgtgggcgag atcatcgccc gacgcctgcg cggcttcgcc
      901 cagggcgccg ccgcgggcat ggtctagaag ccagccttga cctcgatcga caccacgccc
      961 cggggcgagc gcgaattgcg cagatcgccg tcgaccacca cgcgataggg gccttcacgc
     1021 gcgtccagcg gtttgccatt gacggtatcg gccaggatca ccggattggc gcgatagacc
     1081 gggtcggtct cggcgatcga caggatgctg gaaaagccat cggcggcccg cagcacgacg
     1141 atctggttca ggtaccgccc catcagccgc tcgcccgaga cgacgccggc cttcagcagg
     1201 gcgccgtgca gcaccgcgcc ttcatagacc accggctggc ccttgaccgt gatcgtcgcc
     1261 ttggcgcgcg gcagggcctt cacctcagcg ggcgtcagca tcaccgtgtc gcggcccgcg
     1321 accgtgacct tgaggtcctg ggccaggacg ggcgaggcaa gcagggcgat ggcggcggtg
     1381 acggcgagga ggatgcgcat gcgatcagcg tggcgcccct ccccggttct gtcacctatt
     1441 cagctgcgtc atcagcggat acagcgaggc caccagcagc acggccatgc tgacattgaa
     1501 ggtccgcagg atcgctggcc tgtccaggaa ctgccgcagg ccctggccga agcccgccca
     1561 ggcggcgctg cagggcgcgc cgaccagcat gaaggttccc gccagcagca acacggccgt
     1621 gggcccgccg ccctcgggcg cataggtagt caccgcgccc agcgccatgg cccaggcctt
     1681 ggggttaagc cactggaagc cggcggcctc caggaaggtc atgggctggc cgccggcgcc
     1741 tttgtcgttg atcccggttg cggtggcgat cttccaggcc agccacagca tgtaggtggc
     1801 gccgacccat ttgaggatct cgtgcaggat cgggaaggcc tcaaacacgc ccgacaggcc
     1861 ccagcccatc gccaccacca tcacgcccag gccgacgctg atccccagga tgtgcaggat
     1921 ggtgcggcgg aacccgaagt tcgcgcccga cgccagcagc atcatgttgt tgggaccggg
     1981 cgtgccggcg gtggcgaagg cgaacagcac aaaggcgagc agcaggtcgg gggtcatggc
     2041 gaagactctg gatcgagaat gtcacagaga tgttcccgat tgatccatga gtcaaaaaga
     2101 gtattgtcac gatgacaata tcggagaccc catgggcgac tggacaccga ccctcacccc
     2161 aggcgacgcg ccgatctatg agcgcctgct ggacgccctg cgcgccgaca tcgaggccgg
     2221 cgccctggcc gccggcgcgc gcct
//
LOCUS       DAOMEO010000522         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5106_length_2244_cov_2.137049, whole genome shotgun sequence.
ACCESSION   DAOMEO010000522 DAOMEO010000000
VERSION     DAOMEO010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5106_length_2244_cov_2.137049"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLH31_11660"
     CDS             <1..1081
                     /locus_tag="PLH31_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR89889.1"
                     /translation="VRPEDRAISWLPLYHDMGLIGFLLSPMTSQMTVDLLPTGAFVRR
                     PLMWLDLMTKNGGTISYSPTFGYELCARRAEAASIEHLDLSGWRSAGLGGDMIRTGPL
                     RAFAERFKTVGFSDSAFVASYGMAEATLALSMSPLGEGLKVETLDVEQLEQGLAVITT
                     DTDRARDFARNGPVLPHHELEVRGDDGAVLPERGVGRIYARGPSLMREYFEQPEETAR
                     VLSADGWLDTGDLGYLIDGEIVITGRGKDLIILNGRNIWPQDLEWTAEAEIESLRSGD
                     VAAFSTPGAGEEVVVVLVQARGGDPETRKALAENVATVLRLRHSVEVQVHLVGAHALP
                     QTSSGKLSRSKAKAAFLAGAYTERA"
     gene            1084..2034
                     /locus_tag="PLH31_11665"
     CDS             1084..2034
                     /locus_tag="PLH31_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQR89890.1"
                     /translation="MSRGVVAVTGATGFLGRRLVTALFADGWTVRILARRDIIDPDWA
                     GLEPQVVIGGLSDPAALERLCSGAAVIIHVAGLIKARSRAEFDRTNVHGARDVASAAK
                     AAGGRLILVSSLAAREPGLSDYAASKRGGEDAARDAFGERLTIVRPPAIYGPGDMETL
                     GLFKAAQSAPFLPVLDPAARIAMIHVDDAVAAITGLAGDPRSGLFALSDSRTDGYSWT
                     EVMTAAAGAVGKKPHIGRVPTWLIKALARLSEGVSLAIGKISIFTTGKAREMLHLDWS
                     VSDSEMLPRAIAPRYGIESGFAQSVQWYRAKGFLVALREL"
     gene            2120..>2244
                     /locus_tag="PLH31_11670"
     CDS             2120..>2244
                     /locus_tag="PLH31_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR89891.1"
                     /translation="MKAVTDLTLREIGLAASSVLGRSVEVSDETHIGRDLQVDSLA"
BASE COUNT          376 a          730 c          750 g          388 t
ORIGIN      
        1 agtgcggcct gaagaccgcg cgatctcgtg gctgccgctc tatcacgaca tgggcctgat
       61 cggcttcctg ctcagcccca tgaccagcca gatgacggtc gacctgctgc ccaccggggc
      121 cttcgtgcgc cggccgctga tgtggctgga cctgatgacg aagaatggcg gcacgatcag
      181 ctatagcccg accttcggct atgagctgtg cgcccgccgg gccgaggccg cctcgatcga
      241 gcatctggac ctttcgggct ggcgcagcgc cggcctgggc ggcgacatga tccgaaccgg
      301 ccccctgcgg gccttcgcag aacgcttcaa gaccgtaggc ttctccgaca gcgcctttgt
      361 cgccagctat ggcatggccg aggccaccct ggccttgtcg atgtcaccgc tgggcgaggg
      421 cttgaaggtc gagaccctgg acgtcgaaca gctggaacag ggcctggcgg tgatcaccac
      481 cgacaccgac cgcgcgcgcg acttcgcccg caatggccct gtcctcccgc accacgaact
      541 ggaagtgcgc ggagatgacg gcgcggttct gcccgagcgc ggcgttggca ggatctatgc
      601 gcgcggccca agcctgatgc gggaatattt cgagcagccc gaagagaccg cccgcgtgct
      661 gtcggccgac ggctggctgg acaccggtga ccttggctat ctgatcgacg gcgagatcgt
      721 catcaccggg cgtggcaagg acctgatcat cctcaacggc cgcaacatct ggccgcagga
      781 cctggaatgg accgccgagg ccgagatcga gtccttgcgc agcggcgacg tcgccgcctt
      841 ctcgacgccc ggcgcgggcg aagaggtggt ggtcgtgctg gtccaggcgc gcggcggcga
      901 tcccgagacc cgcaaggccc tcgccgagaa cgtcgccacc gtgctgaggc tgcgccacag
      961 cgtcgaggtg caggtgcacc tcgtcggcgc ccacgccctg ccccagacct cctcaggcaa
     1021 gctgagccgc tccaaggcca aggccgcttt cctggccggc gcctataccg agcgcgcctg
     1081 agcatgagcc ggggtgtcgt cgccgttacc ggcgccaccg gttttctggg ccggcgactc
     1141 gtcaccgccc tgtttgccga cggctggacc gtccgcatcc tggctcgccg cgacatcatc
     1201 gatccggact gggcggggct ggagccccag gtcgtgatcg gcgggctgtc ggatcccgca
     1261 gccttggaga ggctttgcag cggcgccgcc gtgatcatcc atgtcgcggg cctgatcaag
     1321 gcgcgctcgc gggcggaatt tgaccgtaca aatgtccacg gcgcccgtga cgtcgccagc
     1381 gcggccaaag cagcgggcgg acggctgatc ctggtttcca gcctggcggc gcgggagccg
     1441 ggtctgtctg actatgcggc cagcaagcgc ggcggcgagg atgcggcccg tgacgccttc
     1501 ggagagcgtc tgacaatcgt gcggccgccc gcgatctatg gcccaggcga catggagacc
     1561 ctgggtctgt tcaaggctgc ccagagcgcg ccgtttctgc ccgtgctcga tccggcggcc
     1621 aggatcgcta tgatccacgt tgacgatgcg gtcgccgcaa tcaccggact ggccggcgat
     1681 cccagatcag gccttttcgc cctgtcggac agccgcaccg acggttacag ctggaccgaa
     1741 gtgatgacgg ccgccgctgg cgcagtgggc aaaaagccac atatcgggcg cgtgccaacc
     1801 tggctaatca aagccttggc gcgattgtcc gaaggcgtct cactcgccat tgggaagatt
     1861 tccattttca cgactggaaa agcgcgcgaa atgctccatc tggattggag cgtttcggac
     1921 agcgagatgc tgccgcgggc tatcgctcca cggtacggga tagagtcagg gttcgcccaa
     1981 agcgtgcaat ggtatcgcgc caaggggttt ttagttgcgt tgagagaact atagcagcct
     2041 attttgacag gattccgaca acagggctgt tacaattcga gtcagttagg cccgctcacg
     2101 ggggcgtcag cgagtcggca tgaaagcggt tacagatctt accttgcgtg aaattggcct
     2161 tgcggcgtcc tcagtgctgg ggcgcagcgt ggaggtttcg gacgagactc acatcggccg
     2221 agacctgcag gtcgattccc tggc
//
LOCUS       DAOMEO010000523         2242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5110_length_2242_cov_2.362597, whole genome shotgun sequence.
ACCESSION   DAOMEO010000523 DAOMEO010000000
VERSION     DAOMEO010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_2242_cov_2.362597"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="PLH31_11675"
     CDS             complement(<1..1965)
                     /locus_tag="PLH31_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR89892.1"
                     /translation="MSNHRKHLLASTLIAGLAVGVMTPALALAQAAAPLATADDQAAE
                     ATEVEALVVTGSRIKRSEFNSPDPIQVISAEQGRLKGISTASGLLQTSTIASGSPQVT
                     SAISSAFITDGGPGAETISLRGLGANRTLVLINGRRTGPAGVRGGVSAVDLNVIPVSA
                     IDRIDILKDGASSIYGSDAVAGVVNIITKRDTDGFEIDAFVSQPQDKGGEQMRGSLNW
                     GKTFDRGFFNMSYDYSKTTEQTIGSRSYTNCGRQYITDSSGKRKDTIDPRTGSAQCRD
                     LLYDQIWLYDFSFGGIDGKLQYDYDGVLGGLIPQVPPGQAANYPGMPAGFYTVGYSDD
                     PQAADNGVLDYNSPFNLASSLVPRTERSTVYAQGAFDINDSIEAYGEVLLNRRATKTN
                     GYRQFWSYFYTGDYDPFSAGFTGDFILSPTPVTNRNRSGQRVDYQRYVGGLRGDFGKI
                     DFLKGWDWDVFTQYSHSKGTYSQDVILQDAVDIGAFRTGSCLGQVTPVSNKQCVDVSW
                     VTPDFLAGKYTAAEEAFLFDNEEGQTTYKQLVIEGSTSGTLFTLPAGDVGAAFGFHYR
                     KDSINDVPGAITLAGNVWGASTAGITKGDDLAREFFGELSVPLLKGLPFAENVELSLS
                     GRHTKINSYGEDDTYKIGLNWQIIPSLRVRA"
     gene            complement(1991..>2242)
                     /locus_tag="PLH31_11680"
     CDS             complement(1991..>2242)
                     /locus_tag="PLH31_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89893.1"
                     /translation="EGVIGSMAMRVFEATIASRLARPTRPLVAQTLIRQAPACADATP
                     CRNYVGYATKTLRANSGRAHLRYGEILLALDEFCRILLR"
BASE COUNT          402 a          661 c          761 g          418 t
ORIGIN      
        1 agcgcggacg cgcagcgacg ggatgatctg ccagttcagg ccgatcttgt aggtgtcgtc
       61 ctcgccatag ctgttgatct tggtgtgacg gccagacagc gacagttcga cattctcggc
      121 gaagggcagg cccttcagca gcgggacgct caactcgccg aagaactcgc gcgccaggtc
      181 atcgcccttg gtgatgccgg cggtggaggc gccccacaca ttgcccgcca gggtgatcgc
      241 gcccggaacg tcgttgatcg agtccttgcg atagtggaag ccaaaggcgg cgccgacgtc
      301 accggccggc aaagtgaaca gcgtgcccga ggtggagcct tcgatgacca gttgcttgta
      361 ggtggtctgg ccttcttcgt tgtcgaacag gaaggcttct tcagcggcgg tgtacttgcc
      421 agccaggaag tccggcgtga cccagctgac gtccacgcac tgcttgttgc tgacgggggt
      481 gacctgaccc aggcacgagc cggtgcggaa tgcgccaatg tcgacggcgt cctgcaggat
      541 cacgtcctgc gaataggtcc ccttggagtg gctgtactgg gtgaagacat cccagtccca
      601 gcccttcagg aagtcgatct tgccaaagtc gccgcgcagg ccgccgacat agcgctggta
      661 gtcgacgcgc tggccagacc ggttgcggtt ggtgaccggg gtcggcgaca ggatgaagtc
      721 gccggtgaag ccagcgctga aggggtcata gtcgccggtg tagaagtagc tccagaactg
      781 gcggtagccg ttggtcttgg tggcgcggcg gttcagcagc acttcgccat aggcttcgat
      841 gctgtcgttg atgtcgaacg cgccctgggc gtagacggtc gaacgctcgg tgcgcggcac
      901 cagcgacgag gccaggttga acgggctgtt atagtccagc acgccattgt cggcggcctg
      961 cggatcatcg ctataaccaa cggtgtagaa gccggccggc atgcccggat agttggccgc
     1021 ctggcctgga ggcacctgcg ggataagacc gcccagcacg ccatcgtagt cgtactgcag
     1081 cttgccgtcg atgccgccga agctgaagtc atacagccag atttgatcgt acagcaggtc
     1141 gcggcactgg gccgagccgg tgcgcggatc gatggtgtct ttccgcttgc cgctggagtc
     1201 ggtgatgtat tgacggccac agttggtgta gctgcgcgag ccgatggtct gctcggtggt
     1261 cttcgaatag tcgtacgaca tattgaagaa gccgcgatcg aaggtcttgc cccagttgag
     1321 gctgccacgc atctgttcgc cgcccttgtc ctggggctgg ctgacgaagg cgtcgatctc
     1381 gaaaccgtcc gtgtcgcgct tggtgatgat gttgacaacg ccggcaacgg cgtccgagcc
     1441 atagatcgac gaagcgccgt ccttcaggat gtcgatacgg tcgatcgcgg acactggaat
     1501 gacgttcagg tcaacggcgg acacgccgcc gcgcacgccg gcgggtccgg tgcggcgacc
     1561 gttgatcagc accagggtgc ggttggcgcc caggccgcgc agcgagatgg tctcagcccc
     1621 ggggccgcca tcggtgatga aggcgctgga gatggccgaa gtgacctggg gcgagcccga
     1681 ggcgatcgtc gaggtctgca gcaggccgga ggcggtcgag atgcccttca gtcggccctg
     1741 ttcggcgctg atcacctgga tcgggtcagg gctgttgaat tcgctgcgct tgatacgcga
     1801 gccggtgacc accagcgctt cgacctccgt cgcctctgcg gcctgatcgt cggcggtggc
     1861 caggggcgcg gccgcctggg ccagcgccag cgccggcgtc atgacgccga cagccaggcc
     1921 ggcgattaac gtggaggcaa gaagatgctt gcgatggttg gacattatag aaagaccccc
     1981 gtgactatat tcaacgtagc aaaatgcggc agaactcgtc taaagcgagc aagatttcac
     2041 cgtaacgcag gtgagcgcgt ccggagttcg cccgcaacgt cttcgtcgca tagccgacat
     2101 aatttcgaca cggtgtggca tccgcgcacg ctggcgcctg cctgatcagc gtttgagcaa
     2161 caaggggacg ggttggccgc gccagccggc ttgctattgt ggcctcgaac acgcgcatcg
     2221 ccatgctgcc aataacgcct tc
//
LOCUS       DAOMEO010000524         2232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5138_length_2232_cov_2.020211, whole genome shotgun sequence.
ACCESSION   DAOMEO010000524 DAOMEO010000000
VERSION     DAOMEO010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5138_length_2232_cov_2.020211"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLH31_11685"
     CDS             complement(<1..741)
                     /locus_tag="PLH31_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HQR89894.1"
                     /translation="MLKVLTCLTTEHDYRLVGVAAVVCFAACFTAFRLYSRMRGAKGL
                     VRAAWLLLTGLVCGSGVWATHFIGMVAYDPGLKTGYSPSGTLLSLMIAVLFMGSGFVV
                     ASAQRTRTNDVAGGLILGMGIASMHYTGMTAFVTQGQLVWEHATVGASVLFGVVGATA
                     ALVIAGRARSLSRQLLGGGILTLAVCALHFTGMGAITIAPDASVMVPDQLLSGGVLTL
                     AVTSITALIILGGLGAVTIESQTTRSALE"
     gene            complement(870..1853)
                     /gene="galE"
                     /locus_tag="PLH31_11690"
     CDS             complement(870..1853)
                     /gene="galE"
                     /locus_tag="PLH31_11690"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQR89895.1"
                     /translation="MQTVLVAGGAGYVGSHTCLALAAAGFKPVVFDDLSNGHSEHVQW
                     GPLEMGDIRDKARLDAVFAVHKPVAVLHFAARIEVGESVRDPGAFFDNNVAGTINLIE
                     AARRAGVEAMVFSSTCATFGDPVKLPMDESHPQAPLNPYGRTKLMVEQALADYGRYVG
                     FRSTVLRYFNAAGADPEGRIGEWHEPETHAIPLAIQVALGQRSHFTIFGDDYDTRDGT
                     AVRDYVHVLDLADAHVAALRRLLGGAQSASYNLGTGHGTTVKELIAGVERATGRPLPV
                     QMAARRPGDAPILVGDNAKARAELGWTPSRDLDVILGSAWRWHQAQADAGR"
     gene            2053..>2232
                     /locus_tag="PLH31_11695"
     CDS             2053..>2232
                     /locus_tag="PLH31_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HQR89896.1"
                     /translation="MRVAMIGTGYVGLVSGACFADFGHVVTCIDKDARKIERLEKGEI
                     PIFEPGLDDLVARNVR"
BASE COUNT          411 a          689 c          763 g          369 t
ORIGIN      
        1 ttcaagggcc gagcgcgtcg tctgggactc gatcgtcacg gcgccgagac cgcccaggat
       61 gatcagcgcg gtgatgctgg tgacggccag ggtcaggacg ccgcccgaca ggagttgatc
      121 tggcaccatg accgaggcgt cgggcgcgat ggtgatcgcg cccatgccgg tgaaatgcag
      181 ggcgcagacg gccagggtca ggatgccgcc acccaggagc tgccggctca ggctgcgggc
      241 gcggcccgca atgacaaggg ccgccgtggc gccgaccacg ccaaacaaca ccgaagcccc
      301 gaccgtggca tgctcccaga ccagttggcc ctgggtcacg aaggcggtca taccggtata
      361 gtgcatggag gcgatgccca tgcccaggat caggccgccg gcaacgtcgt tggtgcgcgt
      421 gcgctgggcc gaggccacga cgaagcccga gcccatgaac aggacggcga tcatcaggga
      481 cagaagggtg ccgctggggc tgtaaccggt tttcaggccc gggtcgtagg cgaccatgcc
      541 aatgaagtgg gtcgcccaca cacctgatcc gcagaccaga cctgtgagca gcagccaggc
      601 ggccctgacc aggcccttgg cgccgcgcat ccgcgagtaa agtctgaacg cggtgaagca
      661 cgcggcgaaa caaacgactg ccgccacgcc gaccaaccga tagtcatgct cggtcgtcag
      721 acaggtcaaa accttcaaca cacgcggttc ccccggtcac atcctcaaac gatacgggga
      781 gagttacaaa taaaccgttt caacgcgggg tcgtgcgaca attggctttg atcgcgcaga
      841 tcgagggccc gattatccct gatggatcgt caccgacctg cgtcggcctg ggcctgatgc
      901 cagcgccagg cgcttcccag gatgacgtcg agatcacgcg acggggtcca gcccagttca
      961 gcccgggcct tggcgttgtc gccgaccagg atcggggcgt cgccggggcg gcgggcggcc
     1021 atctgtacgg gcagaggcct gcctgtggcc cgctcgacgc cggcgatcag ctctttcacc
     1081 gttgtgccgt ggccggtgcc gaggttgtag ctggcgctct gggcgccgcc gagaagccgg
     1141 cgcagggccg cgacatgggc gtcggccaga tcgagcacgt ggacgtagtc gcgcacggcc
     1201 gtgccatcgc gggtgtcgta gtcgtcgcca aagatcgtga agtgcgaccg ctggcccagg
     1261 gcgacctgaa tggccagggg aatggcgtgg gtctccggct cgtgccactc gccgatgcgg
     1321 ccttcgggat cggcgccggc ggcgttgaaa tagcgcagca ccgtagagcg gaagccgaca
     1381 tagcggccat agtcggccag ggcctgctcg accatcagct tggtgcggcc atagggattg
     1441 agcggcgcct gtggatgact ctcgtccatc ggcagtttca ccggatcgcc gaaagtggcg
     1501 caggtcgagg agaagaccat cgcttccacg ccagcccggc gggcggcctc gatcaggttg
     1561 atcgtgccag cgacgttgtt gtcgaaaaag gcgccgggat ccctaaccga ctcgccgacc
     1621 tcgatgcggg cggcgaagtg cagcacggcg acgggcttgt ggacggcgaa cacggcatcg
     1681 agcctcgcct tgtcgcggat gtcgcccatt tccagcggtc cccactggac gtgctcgctg
     1741 tggccgtttg aaagatcatc gaacaccaca ggcttgaacc cggccgccgc cagggcaagg
     1801 caggtatgag agccgacata gccggcgccg ccggccacca gaacggtctg catgaaatcc
     1861 ctttcagcgc tacgagtcct ggctatcggc tttaccttaa cgttgaaaga ctggcgtcat
     1921 gcatcatgac ctcgtcgcaa gaggtccggg cgctggatgt catcgtctgg tggcttgcct
     1981 cggcgctgcg attgctcgat taggcacaag cgtcgctcgc tgggcgatga ttgacctgat
     2041 caggatgtct cgatgcgcgt agcgatgatt ggcacgggtt atgtgggcct ggtgtctgga
     2101 gcgtgctttg ccgatttcgg tcacgtggtg acgtgcatcg acaaggatgc cagaaagatc
     2161 gagcggctgg aaaagggcga gatcccgatc ttcgagcccg gtttggatga tctggtcgcc
     2221 cgcaatgtcc gc
//
LOCUS       DAOMEO010000525         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5139_length_2231_cov_2.662684, whole genome shotgun sequence.
ACCESSION   DAOMEO010000525 DAOMEO010000000
VERSION     DAOMEO010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_2231_cov_2.662684"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLH31_11700"
     CDS             complement(<1..890)
                     /locus_tag="PLH31_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR89897.1"
                     /translation="MRDYTKFYIDGAWVDPVAPKTLDVINPATEEVCGKISMGGVADV
                     DKAVMAARKAFETFSLTSREERIDLLERIVAEYQKRFEDMAKAITEEMGAPAWLAQRA
                     QAAMGIGHVQTAIAVLKNYKFEEDRGTTRIVKEPIGVCAFITPWNWPVNQIACKVAPA
                     LATGCTMVLKPSEIAPFSGYIWTEVLHAAGVPHGVFNLVNGDGPTVGAALSSHPEVDM
                     VSFTGSTRAGIEVAKNAAPTVKRVHQELGGKSPNIILEDADFQRAVSGGIASVMMNSG
                     QSCNAPTRMLVPGKKMDEVIA"
     gene            complement(998..1468)
                     /locus_tag="PLH31_11705"
     CDS             complement(998..1468)
                     /locus_tag="PLH31_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQR89898.1"
                     /translation="MLWRRRIEPFTRPLVYAWFRWNRGLTLGVRGLVTDGEGRVLLIQ
                     HTYIPGWYLPGGGVERGETAQVALARELQEEAGVRVLGPPVLLSVHSNEPRHPGDHVL
                     VYRIDALEPCEACAQGEIHAVGWFAPDALPDDISPGTRRRIAEALEGVDSDVMW"
     gene            1546..1803
                     /locus_tag="PLH31_11710"
     CDS             1546..1803
                     /locus_tag="PLH31_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQR89899.1"
                     /translation="MGDLSWKLEDAKARFSEVVRLARSQGPQRVTVRGKDAVVVMSVE
                     DLERLSPPDPAHPSLVSFLEGLHLDDLDLTRDPDHGRDLEL"
     assembly_gap    1890..1899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1900..2161
                     /locus_tag="PLH31_11715"
     CDS             <1900..2161
                     /locus_tag="PLH31_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89900.1"
                     /translation="ARDALAGRFVGRTLSVSDPVVRRWGEISGAVKRRKGQAPAVIDT
                     LLAATALEHDLFLVTRNTRDVQDSGAAVFNPWTDDPADFPLA"
BASE COUNT          342 a          734 c          761 g          384 t
ORIGIN      
        1 gcgatgacct cgtccatctt cttgcccggc accagcatgc gggtgggggc attgcaggac
       61 tggcccgaat tcatcatcac cgaagcgatg ccgcccgaga cggcgcgctg gaagtcggcg
      121 tcttcgagga tgatgttggg gctcttgccg cccagctcct ggtggacgcg cttgacggtc
      181 ggggcggcgt tcttggccac ctcgataccg gcccgggtcg agccggtgaa cgacaccatg
      241 tcgacttccg gatggctgga cagggccgcg cccaccgtgg ggccgtcgcc attgaccagg
      301 ttgaacacgc cgtgcggcac gccggcggca tgcagaacct cggtccagat atagccgctg
      361 aacggcgcga tttccgaggg cttgagcacc atggtgcagc ccgtggcgag cgcgggcgcg
      421 accttgcagg cgatctggtt gaccggccag ttccacggcg tgatgaaggc gcagacgccg
      481 atcggctctt tcacgatgcg ggtggtgccg cgatcttcct cgaacttgta gttcttgagg
      541 acggcgatgg cggtctggac gtggccgatg cccatggcgg cctgggcgcg ctgggccagc
      601 caggcggggg cgcccatttc ctcggtgatg gctttcgcca tgtcctcgaa gcgcttctga
      661 tattcggcga cgatgcgctc cagcaggtcg atccgctctt cgcggctggt caggctgaag
      721 gtctcgaacg ccttgcgggc ggccatgacg gccttgtcga cgtcggcgac gccgcccatc
      781 gagatcttgc cgcagacctc ttccgtggcc ggattgatca cgtcgagcgt tttgggcgcg
      841 accggatcga cccaggcccc gtcgatatag aacttggtat agtcgcgcat gtcgtgctcc
      901 tgccctgtct gggatgttcg aggtccgctc tcatgtggga cgcggcgggc cttcttcaaa
      961 caaccatttt aatgatcggc gatcatgtgg gaagccctca ccacatcaca tcgctgtcga
     1021 cgccctccag agcctcggcg atccgccgcc gcgtgcccgg gctgatgtcg tctggcaggg
     1081 cgtcgggcgc gaaccagccg accgcgtgga tctcgccctg ggcgcaggct tcgcagggct
     1141 ccaaggcgtc gatgcgatag accagcacgt ggtcgccggg atggcgcggc tcgttggagt
     1201 gcaccgacag caggaccggc gggcccagca cgcggacccc cgcctcctcc tgcagttcgc
     1261 gggccaaggc cacctgggcc gtctcgcccc gctcgacccc gccgccgggc aggtaccagc
     1321 cggggatgta ggtgtgctgg atcagcagca cccggccctc gccgtcggtg accagacccc
     1381 gcaccccaag cgtcagcccc cggttccagc gaaaccaagc atagaccagg ggacgggtga
     1441 acggttcgat gcgacggcgc cagagcatgg gaggcaggat agtcgtcctt gcccgccggg
     1501 cccatccgaa ctatgatctg actagctagt cagatggagc ctgcgatggg cgatctcagc
     1561 tggaaactgg aagacgccaa ggcccgcttc agcgaggtgg tgcgtctggc ccgaagccag
     1621 ggcccgcaac gggtgaccgt gcgcggcaag gacgccgtgg tggtgatgag cgtcgaggat
     1681 ctggagcgcc tgtcgccgcc cgacccggcc cacccgtcgc tggtctcctt tctggagggg
     1741 ctgcacctcg acgatctgga cctgacccgc gaccctgacc acggccgaga tttggaatta
     1801 tgaagggtgg ggacctgtga agggctggct gatcgacacc aatgtgatcg ccgccctgat
     1861 caatccgaac ggcgcgccca gcgtgaaggn nnnnnnnnng gcccgcgacg ccctggccgg
     1921 gcggttcgtc gggcgcaccc tgtcggtcag cgatccggtg gtgcgccgct ggggcgagat
     1981 ctcgggtgcg gtcaagcggc ggaagggtca ggcgcccgcc gtgatcgaca ccctgctggc
     2041 cgcgacggcg ctggagcacg acctgttcct ggtcacccgc aacacccgcg acgtgcagga
     2101 cagcggcgcg gcggtgttca atccctggac cgacgatccc gccgattttc cgttggcgtg
     2161 agcccgctct tgccccgggc ttcgcgcctc gctaaagccg ctgcatcctt ggtcttcaac
     2221 ggagccgcgt c
//
LOCUS       DAOMEO010000526         2231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5140_length_2231_cov_2.251838, whole genome shotgun sequence.
ACCESSION   DAOMEO010000526 DAOMEO010000000
VERSION     DAOMEO010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_2231_cov_2.251838"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..215)
                     /gene="ccoS"
                     /locus_tag="PLH31_11720"
     CDS             complement(51..215)
                     /gene="ccoS"
                     /locus_tag="PLH31_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQR89901.1"
                     /translation="MNIILFLAPFSVGLGALGLVAFLWTMRVGQYDDPKGDAARVLYD
                     HLDDGPPSDN"
     gene            complement(212..>2231)
                     /locus_tag="PLH31_11725"
     CDS             complement(212..>2231)
                     /locus_tag="PLH31_11725"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQR89902.1"
                     /translation="GVAGVRLNLTTGKLAVDLKAASVEPDQVVQAVEALGYRASLFDP
                     TEATAAQDREGRELAVALGVAGFGAGNVMMFTVPAWAGLFGQELNASTLTVMYWMAAI
                     VATPCALFAGRPFFRSAWASLRRGKANMDVPISIGVLLTLIVSFSETLLNGRHAYFDA
                     AVTLLFLLLIGRYLDHRLRASARSAAHDLLVLQAPVAMRVHNGVEQGVPVAQVQVGDQ
                     LAVAPGQRAPVDGYVDSGVSEIDNALISGETALVAVGPGARIHAGALNLTGRLVMTAA
                     ARSEDSTLAAIARLMEAGAQSRSAYVRLADKAAALYVPIVHAAAALTFLVGWAIGLGP
                     REALLRAAAVLIVTCPCALGLAVPAVQIAASARLFRKGVLVKSGAALERLAEVDHVVF
                     DKTGVLTEGLPRLVDAPAHLVALAAPLARASRHPLARAMAREAGVGLVAQACVETAGQ
                     GVEGLIDGRRARLGRAAFVGVSPGDERETELWFGFDGDTKIRFVFEDKSRAGAAQAIA
                     DLRAQGLEVEVLSGDLPGPVSRIAAELGISAWRAGLTPFEKAEAIDALKARGRKVLMV
                     GDGLNDAAALARAHASMAPGAAVDAAQNAADLVFTGEALDAVPTAIGVARSARGRALQ
                     NFTFSALYNLVAAPAAMFGLINPFIAALAMSGSSMVVLLNALRPGWRR"
BASE COUNT          404 a          800 c          735 g          292 t
ORIGIN      
        1 cgatccgaca accgtttcac acttatcgga aaatgctcta gccgagctga tcagttgtcc
       61 gatggcggtc cgtcatccag atggtcgtag agcacgcgcg ccgcgtcgcc tttcggatcg
      121 tcgtactggc ccacccgcat ggtccacagg aaggctacca atcccagggc gcccagcccg
      181 accgaaaacg gcgcgaggaa caggatgatg ttcatcgccg ccaccccgga cgcagcgcgt
      241 tgagcagaac gaccatcgag gatccggaca tcgccaaggc ggcgatgaac ggattgatca
      301 ggccgaacat ggcggcaggc gcagccacca gattgtaaag cgcagagaac gtgaagttct
      361 gcagcgcccg gccccgcgcc gagcgggcca cgccgatcgc tgtgggaacc gcgtccagcg
      421 cctcgccggt gaacaccagg tcggcggcgt tctgggcggc gtcgacggct gcgcccgggg
      481 ccatcgaggc gtgagcccgc gccagggcgg ccgcatcgtt gaggccgtct cccaccatca
      541 gcaccttgcg gcccctagcc ttcagagcgt cgatcgcctc ggccttctcg aacggcgtca
      601 ggccggcgcg ccaggcggag atccccagtt cggcggcaat ccgggaaaca ggtcccggca
      661 ggtcccccga cagcacctcg acctccaggc cctgggcgcg gagatcggcg atcgcctggg
      721 cggcgccggc gcgcgacttg tcttcgaaga caaagcggat cttggtgtcg ccgtcgaagc
      781 cgaaccacag ttcagtctcc cgttcatcgc caggactgac gcccacaaag gcggctcggc
      841 ccaggcgcgc gcgtcgcccg tcgatcagac cttccacgcc ctgccctgcg gtctcgacac
      901 aggcttgggc gaccagaccc acgcccgctt cccgcgccat ggcgcgggcc aggggatggc
      961 gcgaggcgcg ggccagcggc gccgccagag cgaccagatg ggccggtgcg tcgaccagac
     1021 gcggcaggcc ttcggtcagc accccggtct tgtcgaacac cacgtgatcg acctcggcca
     1081 gtcgctccag cgcggcgccg gacttgacca gcacgccctt gcggaaaagc ctggcgctgg
     1141 cggcaatctg tacagccggt acggccaggc ccagcgcgca ggggcaggtt acaatcagca
     1201 ccgcggcagc ccgcagcagg gcttctcgcg gcccgaggcc tatcgcccag ccgaccagga
     1261 acgtcagggc ggcggcggcg tgcacgatcg gcacatagag cgcggcagcc ttgtcggcca
     1321 ggcgcacata ggccgaacgg ctctgggcgc ccgcttccat caggcgcgcg atcgccgcca
     1381 gggtggagtc ctcgctgcgc gcggcggcgg tcatgacaag tcgcccggtc aggttcaggg
     1441 cgccggcatg aatgcgcgcc ccggggccga cagcgaccag cgccgtctcg cccgagatca
     1501 gcgcgttgtc gatctctgaa accccggaat ccacatagcc atcgaccggc gccctttgtc
     1561 cgggcgcgac cgcgagctga tcgccgacct gcacctgagc cacgggaacg ccctgctcca
     1621 cgccattgtg aacccgcatg gccaccggcg cttgcaggac cagcaggtca tgagccgccg
     1681 agcgggcgct ggcccgcagc cggtgatcga gatagcgacc gatcagaagc agaaacagca
     1741 gggtgacggc cgcgtcgaaa taggcgtggc ggccattcag caacgtctcg gaaaaactga
     1801 caatcagggt cagcaggacc ccgatcgaga tcggcacgtc catattggcc ttgccgcgcc
     1861 gcagcgacgc ccaggccgac cggaagaacg gccggcccgc gaagagggcg caaggcgtcg
     1921 cgacgatggc cgccatccag tacatcaccg tcagggtcga ggcgttcagc tcctggccga
     1981 acagacccgc ccaggccggg acggtgaaca tcatgacgtt gcccgccccg aaaccggcga
     2041 cgcccagcgc cacggccagt tcgcggcctt cacggtcctg agccgcagtc gcctcggttg
     2101 gatcgaacag agaggcgcga tagccgagcg cctcaacggc ctgcaccacc tggtcgggtt
     2161 caaccgacgc cgccttgaga tccaccgcca gcttgccggt tgtcagattc aggcgcacgc
     2221 cggcgacgcc c
//
LOCUS       DAOMEO010000527         2229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5145_length_2229_cov_2.934683, whole genome shotgun sequence.
ACCESSION   DAOMEO010000527 DAOMEO010000000
VERSION     DAOMEO010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_2229_cov_2.934683"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..299
                     /locus_tag="PLH31_11730"
                     /pseudo
     CDS             1..299
                     /locus_tag="PLH31_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519013.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     assembly_gap    300..309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <310..720
                     /locus_tag="PLH31_11735"
     CDS             <310..720
                     /locus_tag="PLH31_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HQR89903.1"
                     /translation="RIFAIGDVSHRPLPLYERQFRLESVPNALEQAKQAAAAIVGRPG
                     PTPEVPWFWSDQYDLKLQIAGLPFDADRIIVRGDVTAAKFAVFHMKGDLIQAVEAVNS
                     PPEFMAGKQLIAKRTPVSVEKLADATVSMKDVAA"
     gene            768..>929
                     /locus_tag="PLH31_11740"
     CDS             768..>929
                     /locus_tag="PLH31_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR89904.1"
                     /translation="MAKITYIEHDGTEHALDVKSGLTVMEGAVKNNVPGIDADCGGAC
                     ACATCHVYVD"
     assembly_gap    930..939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <940..1091
                     /locus_tag="PLH31_11745"
     CDS             <940..1091
                     /locus_tag="PLH31_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S ferredoxin"
                     /protein_id="HQR89905.1"
                     /translation="LSKTGDKSAMEESMLDFAENVEPNSRLSCQIKVSDALDGLIVRL
                     PESQH"
     gene            1929..>2229
                     /locus_tag="PLH31_11750"
     rRNA            1929..>2229
                     /locus_tag="PLH31_11750"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          445 a          616 c          696 g          452 t
ORIGIN      
        1 ggcccgcgct ctgggcagcc acgccatgat tatcgagcgc gaggaccggg tcctggcccg
       61 cgtcgcctgt catgcgctgt cgtcgttctt tcaggactat cacagcgcgc ggggcgtcaa
      121 ttttgagctc aatgccggtg tcgagtcctt cgaaggcgcg gatggtcacg tgaccggcgt
      181 ccgcttctct gatgggcgcc tcgttgcgtg cgacgcggtg ctggtcgggg ttggcgccat
      241 tcccaatgac gatctggcca aggacgcggg tctcgactgc gccaatggcg tggtggtggn
      301 nnnnnnnnnc gcattttcgc gatcggcgat gtcagccatc gacctctgcc gctctatgag
      361 cgccagttcc ggctggagag tgtgcccaac gcgctggagc aggccaagca ggcggccgcc
      421 gccattgtcg gtcgcccggg tccgacgccc gaagtgccat ggttctggtc ggaccagtat
      481 gacctgaagc tgcagatcgc cggcctgccg ttcgacgccg accgcatcat cgtacggggc
      541 gacgtgacgg cggcgaagtt cgcggtcttc catatgaagg gcgacctgat ccaggccgtc
      601 gaagcggtca attcgccgcc cgaattcatg gcgggcaagc aactgatcgc caaacgcacc
      661 ccggtctcgg tggaaaaact cgccgacgcc acggtatcaa tgaaggacgt ggcggcctga
      721 aagccgccca tagaggggag cccccaacgg ggaagcaagg acagcgcatg gccaagatca
      781 cctatatcga gcacgacggc acggaacatg ctctcgacgt caagtctgga ctgacggtca
      841 tggagggcgc ggtgaagaac aacgtgccgg gtatcgacgc cgattgcggc ggggcgtgcg
      901 cctgcgccac ctgtcacgtc tatgtcgatn nnnnnnnnng gctttcgaag acgggcgaca
      961 agtcggccat ggaggagtcg atgctcgact tcgccgagaa cgtcgagccc aacagccgtc
     1021 tgtcctgcca gatcaaggtc agcgacgcgc tggacggcct gatcgtccgg ctcccggaaa
     1081 gccagcatta agtaagccgc ccagaaagcg tcgttatatc gtcacgaagg gcttgccatg
     1141 agcggggcgc gggactaaat ccgcccctcg ctcggaaccg catcgcggcc ggacggcgcg
     1201 gccccgaaag ggcgcttgca tcgcctgtgt cgcttcgtta gtttccgcgc tccaatcgac
     1261 tcggtgatgg acacacgagg gaagcctagg cggccctggt ccatattctt gcgctccgtt
     1321 tcccggtttt ccggggttcg aaaagttcga aacgagttgg ttgacttcgg agtgtcgcct
     1381 ctttagaagc gccgctccgg gcatcgccgc caagtcttcg caagaattca gcggcgcggt
     1441 gtcaagtcta tcaaccagtt gttctgaagt ttcttcaaaa taatcggttg gctgactagg
     1501 ttagcttctt tagaagctgc ccactctggc gggtccggga tttcccgggt tggccgctcc
     1561 ggctcaaaac gttttgcaaa aaacgatttg acatggaatt cgaggttgga tagatagccg
     1621 cctccgccga catcgggcgc taaacggatg gttctgccga gaggcggttc tgggagcttc
     1681 ggctctgggt ctttgacatt gttgatttgg aaagagaaac gcaggcggcg gcgctctggc
     1741 gatggactga aaggttcatc tcgaacgctg acaaatgcgg tctcttgaag acaccatgaa
     1801 tatcattgga ctgaagactt cggttttcaa tccatcgatg tgattgggaa ctcgtcaaga
     1861 tactatgcaa accagaactg gatcgtgggt tttcccccat gatctgaagt ctgtgttagc
     1921 ggtcaactca acctgagagt ttgatcctgg ctcagagcga acgctggcgg caggcctaac
     1981 acatgcaagt cgaacggatc cttcgggatt agtggcggac gggtgagtaa cacgtgggaa
     2041 cgtgcctttt ggttcggaac aactcaggga aacttgagct aataccggat aagcctttcg
     2101 agggaaagat ttatcgccat tagagcggcc cgcgtaggat tagctagttg gtggggtaaa
     2161 ggcccaccaa ggctacgatc cttagctggt ctgagaggat gatcagccac actgggactg
     2221 agacacggc
//
LOCUS       DAOMEO010000528         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5158_length_2226_cov_2.445877, whole genome shotgun sequence.
ACCESSION   DAOMEO010000528 DAOMEO010000000
VERSION     DAOMEO010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5158_length_2226_cov_2.445877"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLH31_11755"
     CDS             <1..225
                     /locus_tag="PLH31_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQR89906.1"
                     /translation="WEQVEAAEQRGWASDQGYFTKGVQTIAAPVFDRAGDVAFTIVGV
                     MLLGQYADEALDQIGVALRDAGQRLTTALT"
     gene            complement(239..652)
                     /locus_tag="PLH31_11760"
     CDS             complement(239..652)
                     /locus_tag="PLH31_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR89907.1"
                     /translation="MIRRMDHFTVVTDRPEETIAFYARLGLSTGPRPDFGIDGAWLYT
                     GGHPVLHIVETTVMPEPRRGALDHMAFFAEGFAAVAERITAASLTYRVIRTPRPFSQW
                     QMFLFDPNGVEVELDFAAEETPPPDWKAHAGLGAR"
     gene            827..>1334
                     /locus_tag="PLH31_11765"
     CDS             827..>1334
                     /locus_tag="PLH31_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR89908.1"
                     /translation="MTVDDMILVSVDDHVIEPPNAFTRHWPERLKGREPHIEERDGRD
                     VWMFEDKAAGYMGLNSVVGRPKEEYGMEPLNYAQMRRGTWDIKARVEDMDANGILGSI
                     CFPTFPGFAGARFQTASKQDPDVALAAIRAYNDWHIHDWAGAAPGRFIPLMLTPFWDM
                     QAAVAEVER"
     assembly_gap    1335..1344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1345..2128
                     /locus_tag="PLH31_11770"
     CDS             <1345..2128
                     /locus_tag="PLH31_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR89909.1"
                     /translation="ALAGWPSLHDPYWDPLWKACADNQVVICCHIGTGSAAAHASDLS
                     PIDAWITSMPISIANSAADWIWAAMWKKYPALKMALSEGGIGWIPYLLERADFTHGHH
                     NAWTNSNFGPGVMPSDIYKKHIISCFIEDKFGLANLDYIGEDMVMYECDYPHSDSVWP
                     NSAEKLWNDVQHLSRETIDKITHINAMREFSYDPFSVLKKEDCTVGALKAAAAAVPVH
                     TDPLLNLGGAAPKREAGKPVTSGDINRMFANASAESTVSGRR"
BASE COUNT          413 a          705 c          717 g          381 t
ORIGIN      
        1 tgggaacagg tcgaggccgc tgaacaacgg ggctgggcca gcgaccaggg ctattttacc
       61 aaaggggttc agacgatcgc ggcgccggtc ttcgaccgcg cgggcgacgt ggcgttcacg
      121 atcgttggcg tcatgctgct gggacaatac gcggatgaag ccctcgatca gatcggcgta
      181 gccctgcgcg atgcgggcca acgtctgacg acagccctga cctgagcgag gcccgcgcct
      241 agcgggcgcc gaggccggcg tgggctttcc aatccggcgg cggggtctcc tcggccgcga
      301 aatccaattc gacctcgacg ccgttggggt cgaacagaaa catctgccac tggctgaacg
      361 ggcgaggcgt gcggatcacg cggtaggtca gtgaagcggc ggtaatccgc tcggccacgg
      421 cggcgaagcc ttcggcgaag aacgccatgt gatcgagcgc gccgcgcctc ggttcgggca
      481 tcaccgtcgt ttcgacgatg tgcagaacgg gatgcccgcc cgtgtagagc catgcgccgt
      541 cgatgccgaa atccggccgg gggccggtgc tgagccccag cctcgcatag aaggcgatcg
      601 tctcttccgg ccggtcggtc acgaccgtga aatggtccat gcgacggatc atgtcctctc
      661 cttgagcaag gtctggggtg ggagagtggt gcgccgaccc agggatattt tctagacact
      721 tttgcgattt ttgttataga aaggaattct gaatagcggc ggcgcccagg gctccgccca
      781 gtcgagaggc ggcgccaagc cgccctttga aagtgaggaa gagctgatga ctgtcgacga
      841 catgatcctt gtgagcgtgg acgaccacgt catcgagccg ccaaatgctt tcactcgaca
      901 ctggcccgaa cgtctgaagg ggcgcgagcc gcacatcgag gagcgcgacg gtcgcgatgt
      961 ctggatgttt gaggacaagg ccgccggtta tatgggcctg aactccgtcg tcggccgccc
     1021 gaaggaagag tacggcatgg agccgctcaa ctacgcgcag atgcggcgcg gcacctggga
     1081 catcaaggcg cgcgtcgagg acatggacgc caacggcatc ctgggctcaa tctgctttcc
     1141 gaccttcccg ggcttcgccg gcgcccgctt tcagaccgcg tcgaagcagg atcccgatgt
     1201 cgccctggcg gcgatccgcg cctataacga ctggcatatc catgactggg ccggcgcggc
     1261 gccggggcgg ttcattccgc tcatgctgac gccgttctgg gacatgcagg cggccgtcgc
     1321 tgaagtggag cggannnnnn nnnnggccct ggcgggatgg ccgtctctgc acgatcccta
     1381 ctgggatccg ctctggaaag cctgcgccga caatcaggtc gtcatctgtt gccatatcgg
     1441 cacgggatcg gccgccgcgc acgcctcgga cctttccccg atcgacgcct ggatcacttc
     1501 gatgccgatc tcgatcgcca attcggcggc cgactggatc tgggcggcca tgtggaaaaa
     1561 gtaccccgcc ctgaagatgg ctctgtcgga gggcggcatc ggctggattc cgtatctgct
     1621 ggaacgcgcc gacttcaccc acggccacca caacgcctgg accaattcga acttcggccc
     1681 cggcgtgatg ccgagcgaca tctataagaa gcacatcatc agctgcttca tcgaggacaa
     1741 gttcggcctc gccaacctcg actacatcgg cgaggacatg gtcatgtacg aatgcgacta
     1801 tccgcactcg gactcggtct ggccgaactc ggccgagaag ctgtggaatg acgtccagca
     1861 cctgtcgcgc gagacgatcg acaaaatcac ccacatcaac gcaatgcgcg agttttccta
     1921 cgacccgttc tcggtgctca agaaggaaga ctgcaccgtg ggcgcgctca aggccgccgc
     1981 ggccgcggtg cccgtccata ccgatccgct gctgaacctc ggcggcgcgg cccccaagcg
     2041 ggaagccggc aagccggtca cctcgggcga catcaaccgg atgttcgcga acgcctcggc
     2101 ggaatcgacg gtttcgggcc gccgctagaa cgaccgaaga ctgaaacgcc cgccggctta
     2161 agtcccggcg ggcgttttca tgtccgcttt ccttcgatcc aacgcctggc gcggccgagc
     2221 agccgg
//
LOCUS       DAOMEO010000529         2226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5159_length_2226_cov_2.152464, whole genome shotgun sequence.
ACCESSION   DAOMEO010000529 DAOMEO010000000
VERSION     DAOMEO010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_2226_cov_2.152464"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PLH31_11775"
     CDS             <1..1154
                     /locus_tag="PLH31_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR89910.1"
                     /translation="NRGDVVVVTVNHRLNAFGYLYLARIFNDPSVADSGNVGQMDLVL
                     ALEWVRDNIAAFGGDPANVTVFGQSGGGAKIATLMAMPTAKGLFHRAATMSGQQVTVG
                     GPFNATKRAQLFLDKLGVRELAQLRALPAEQLLNGLKAIDPVAGSGGVYMGPVLDERS
                     LTRHPFFPDAAPQSLDVPMMIGNTHDETRGFVGYDKKMLNLTWDEVIARLPGQFNARI
                     DIDPATVVAAYRRIYPDYSPSDVFFAASTAGRSWKAAIIQAEERARAGAPAYVYQVDW
                     RSPRDGGVWGAPHTIDIALVFGTLTAKGSLADGGADSIAMSNPMSDAFIAFARTGNPN
                     TPAIPAWEPYTLPRRQTMVFNTPASTMADDPRGAERELFNKVPFIQFGT"
     gene            complement(1155..>2226)
                     /locus_tag="PLH31_11780"
     CDS             complement(1155..>2226)
                     /locus_tag="PLH31_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89911.1"
                     /translation="HIDALKFDPSDPTQTSLALFLTRFVTHFCAPTFVFLSGASVWFQ
                     LARGKSKPALSRFLLTRGIWLILLELFVVSLAIGLRPGLFFLQVIWCIGLGMVLLSAM
                     IWLPRVASLVLGAVIVIGNGLLLLVPPDQLGAAAPLFSLATRPGLFPVGVGLFILYPF
                     LAWIGIMLLGYGLAPVFLMDKARRTRWLLILGSGAIVAFLIIRGLNGYGDPAPWKVQA
                     DGLRTAMSFFQISKYPPSLAYALITLGPALLMLLVLERLRGPAAKVLVTFGRVPLFYY
                     LLHFFAAHLLAAAVGVAIGYPFSAFVWGPNGPDVAALQGWGFPLGVVYLIWIAVVAAL
                     YPLCLWWAGLKARRSDWWLSYL"
BASE COUNT          429 a          782 c          721 g          294 t
ORIGIN      
        1 ccaatcgcgg cgacgtggtg gtggtcaccg tcaaccaccg gctcaacgcc ttcggctacc
       61 tctacctggc ccgcatcttc aatgatccca gcgtcgccga cagcggcaat gtcggccaga
      121 tggaccttgt cctggccctg gaatgggtgc gcgacaacat cgcggcgttc ggcggcgatc
      181 cggccaatgt cacggtgttt ggacagtccg gcggcggagc caagatcgcc acgctgatgg
      241 ccatgccgac cgccaagggc ttgttccacc gcgccgccac catgagcggc cagcaggtga
      301 cggtcggcgg cccgttcaac gccaccaagc gggcgcagct gttcctcgac aagctggggg
      361 tccgcgagtt ggcgcaattg cgcgccctgc ccgccgagca gctgctgaac ggtctgaagg
      421 ccatcgaccc cgtcgccggc tcgggcggcg tctatatggg tccggtgctg gacgagcggt
      481 ccctgacccg ccacccgttc ttccccgacg ccgcgcccca gagcctggat gtgccaatga
      541 tgatcggcaa cacccacgac gagacgcgcg gcttcgtcgg ctacgacaag aagatgctga
      601 acctgacctg ggacgaggtg atcgcccgcc tgcccggcca gttcaacgcc cgcatcgaca
      661 tcgaccccgc cacggtggtc gcggcctatc gcaggatcta tccggactat tcgccgtcgg
      721 acgtgttctt cgccgcctcg acggccggcc gctcgtggaa ggccgcgatc atccaggccg
      781 aggagcgggc cagggctggc gcgccggcct atgtctatca ggtcgactgg cgctcgcccc
      841 gcgacggcgg cgtctggggc gcgccccaca ccatcgacat cgccctggtg ttcggtacgc
      901 tgacggccaa gggctcgctg gccgacggcg gggcggactc gatcgccatg tccaacccga
      961 tgagcgacgc cttcatcgcc tttgcccgca ccggcaatcc caacactccc gcgatcccgg
     1021 cctgggagcc ctacaccctg ccccgccgtc agacgatggt gttcaacacg ccggcctcga
     1081 cgatggccga cgacccgcgc ggggccgagc gggagctgtt caacaaggtg ccgttcatcc
     1141 agttcggcac ctagtcagag atagctcagc caccagtcgg atcgccgcgc cttcagcccc
     1201 gcccaccaca ggcacagcgg atagagcgcg gcgaccacgg cgatccagat gagatagacc
     1261 acgcccagcg ggaagcccca gccctgcagg gcggccacgt ctgggccgtt cggaccccag
     1321 acgaaggccg agaacggata acctatcgcc acgcccacgg ccgccgccag caggtgcgcc
     1381 gcgaagaagt gcagcaggta gtagaacaac ggcacgcgcc cgaaggtcac cagcacctta
     1441 gccgctggcc cacgcaggcg ctccaagacc aacagcatta ggagcgccgg ccccagggtg
     1501 atcagcgcat aggccagcga cggcggatac ttgctgatct ggaagaacga catcgccgta
     1561 cgcaagccgt cggcctggac cttccacggc gcgggatcgc catagccgtt taggccgcgg
     1621 atgatcagga aggcgacaat ggcgccgctt cccaggatca acagccagcg ggtgcggcgg
     1681 gccttgtcca tcaggaacac aggcgcgagg ccgtagccca gcagcatgat cccgatccag
     1741 gccaggaagg gatagaggat gaacaggccg actccgaccg ggaacaggcc cggccgcgtg
     1801 gccagcgaga acagcggggc cgcagcgccc agctggtcgg gcgggacgag cagcaacagg
     1861 ccgttgccga tgacgatcac agcccccagc accagcgatg cgacgcgcgg cagccagatc
     1921 atcgccgaca gcagcaccat gcccagaccg atgcaccaga tcacctgcag gaagaacagc
     1981 cccggcctca ggcctatggc caggctgacc acgaacagct ccagaaggat cagccagatc
     2041 ccccgggtca gcaggaagcg gctcagcgcc ggcttgctct tgccccgcgc cagctggaac
     2101 cagacagagg cgccggacag gaagacgaag gtcggggcgc agaaatgggt gacgaaccgg
     2161 gtcaggaaca gcgccaggct ggtctgggtc ggatcgctgg gatcgaactt cagcgcgtcg
     2221 atgtga
//
LOCUS       DAOMEO010000530         2223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5174_length_2223_cov_2.001384, whole genome shotgun sequence.
ACCESSION   DAOMEO010000530 DAOMEO010000000
VERSION     DAOMEO010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5174_length_2223_cov_2.001384"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLH31_11785"
     CDS             <1..245
                     /locus_tag="PLH31_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89912.1"
                     /translation="LGGAMVSEAAAAGLHALTVPWFAAGAVGALLGAIWNFGAVQRLT
                     WRRLTAAERARALVVSPAGPAADRSPSGLHIPIFGN"
     gene            complement(302..565)
                     /locus_tag="PLH31_11790"
     CDS             complement(302..565)
                     /locus_tag="PLH31_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89913.1"
                     /translation="MAASAIKDTDVVRRRCRFMIVSPQETPMLGHICFFQRRQKSPQQ
                     RFLALLTNNLAAKMTVAVTLVCERVKGKIDPKFKNDSAVYGKT"
     gene            510..>2223
                     /locus_tag="PLH31_11795"
     CDS             510..>2223
                     /locus_tag="PLH31_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR89914.1"
                     /translation="MKRQRRLTTSVSLIALAAMLTAGQAYARQSTDNQTVDEVVVTGI
                     RASLERAIQIKRTNSGVVDAISAEDIGKFPDTNLAESLQRITGVSIDRTNGEGSQVTV
                     RGFGGGFNLVTVNGRTMPTANVATVGGDQSADFAGGTSRSFDFSNLASEGVTTLEVYK
                     TGRAAIPSGGIGATINVKTRRPLDGKSGLSGSLGIKGVYDEGVDKSQEGADKITPEIS
                     GLLNWLDDSEKFGIGLFGSYQKRNFTGRSATSNDWNIRRYSEFINPANGFVRNNGTTQ
                     ITNAPANGNVLVAIPNDSRYHFAEGERERINGQLSVQFRPTDNLTLTGDVLYAQNKAY
                     ERRNDSTNWFNRPFDRVTFDSNPVVATAVLLQETLSGTKDMGFEQQYRANKDTLESYG
                     LNAEWALSDTFRVNFDGHVSKTDSSPDAPNGTSMTSVSIGAPIVSSHSVDYSGKVPVQ
                     TFTINDAAPRGNANGTLDVGDLGSQVARTSAQRQQHDIKEFRADATWDLDDNGSRFDF
                     GVDYRTSKMVQSNINTQQDLGSWGISNPRDVSQYAGSLVTSFCLACQFNTYEPNQSGA
                     GLTSF"
BASE COUNT          445 a          734 c          646 g          398 t
ORIGIN      
        1 gtctgggcgg tgcgatggtg agcgaggccg ccgccgccgg gttgcatgcg ttgactgtcc
       61 cttggttcgc cgccggcgca gtcggcgcct tgcttggcgc gatctggaac ttcggcgctg
      121 tacagcgtct cacctggcgg cggctgacgg ccgcggaacg cgcccgggcg ctggttgtaa
      181 gccccgcggg acctgcggcg gacaggtcgc ccagcgggtt gcacatcccg atcttcggaa
      241 actgacatat cgtcgaaatt cctaatgccg caacaatctg ttcaaacgac tgttcgtttg
      301 gtcaagtttt cccataaaca gcgctgtcat ttttgaattt tgggtctatc ttgcccttga
      361 ccctctcgca gaccaatgtt accgctaccg tcatcttcgc tgctaggttg ttcgtgagca
      421 gggctaaaaa acgctgctgc gggctttttt gacggcgctg aaaaaaacaa atgtgcccca
      481 acatcggggt ctcttgcggg gaaacgatca tgaagcggca acgacgcctc accacatctg
      541 tatctttgat agcgctggca gccatgctta cagcgggtca agcctatgcg cgacagtcga
      601 cggacaacca gaccgttgac gaggtggtgg tcaccggcat tcgcgcttcg cttgaacgcg
      661 ccattcagat caagcggacc aacagcggcg ttgtcgatgc gatctcggcc gaagacatcg
      721 gcaagttccc cgacaccaat ctggcggaat cgctccagcg catcacgggc gtctccatcg
      781 accgcaccaa cggcgaaggc tcgcaagtta cggttcgcgg cttcggtggc ggcttcaatc
      841 tggtgacggt caacggtcgc accatgccga ccgccaacgt cgccaccgtc ggcggcgacc
      901 agtcggcgga cttcgctggc ggcaccagcc gttcgttcga cttctccaat ctcgcctcgg
      961 aaggcgtgac gacgcttgaa gtctacaaga ccggccgtgc ggccatcccg tcgggcggca
     1021 tcggcgccac gatcaacgtc aagacccgtc gtccgctgga cggcaagtcc ggcctgagcg
     1081 gcagcctggg tatcaagggc gtctatgacg agggcgtcga caaaagccag gaaggcgccg
     1141 acaagatcac gccggaaatc tcgggtctgc tcaactggct cgacgactcc gagaagttcg
     1201 gcatcggcct gttcggcagt tatcagaagc gcaacttcac gggccgcagc gccacgtcca
     1261 atgactggaa tatccgccgc tactcggagt tcatcaaccc ggccaacggc ttcgtccgca
     1321 ataacggtac gacccagatc accaacgcgc cggccaatgg caacgtcctg gtcgccatcc
     1381 ccaacgacag ccgctaccac ttcgccgaag gcgagcgcga gcgcatcaat ggccagctgt
     1441 cggtgcaatt ccgtccgacc gataacctga ccctgaccgg cgatgtgctg tacgcgcaga
     1501 acaaggccta tgagcgccgt aacgacagca ccaactggtt caaccgtccg ttcgaccgcg
     1561 tcaccttcga cagcaacccc gtggtcgcca cggccgtgct gctgcaggaa acgctcagcg
     1621 gcacaaagga catgggcttc gagcaacagt accgcgccaa taaggacacg ctggaatcct
     1681 atggcctgaa cgccgagtgg gccctcagcg ataccttccg ggtgaacttc gacggccatg
     1741 tctcgaagac cgacagctcg cctgatgctc ccaacggcac cagcatgacc tcggtcagca
     1801 tcggcgcgcc catcgtttcg tcgcactcgg tggactacag cggcaaggtc ccggttcaga
     1861 ccttcacgat caacgacgcc gcgccgcgcg gcaacgccaa cggcacgctg gatgtcggcg
     1921 atctgggcag ccaggtggcc cgcacctcgg cgcagcgcca gcagcatgac atcaaggaat
     1981 tccgggccga cgccacctgg gatctcgatg acaacggcag ccgcttcgac ttcggtgtcg
     2041 actaccgtac gtccaagatg gtccagtcga acatcaacac ccagcaggac ctgggcagct
     2101 ggggtatctc caacccccgc gacgtctcgc agtatgcggg cagcctggtc accagctttt
     2161 gcctggcctg ccagttcaac acctatgagc ccaaccagtc gggggccggc ctgacctcgt
     2221 tcc
//
LOCUS       DAOMEO010000531         2219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5185_length_2219_cov_2.573937, whole genome shotgun sequence.
ACCESSION   DAOMEO010000531 DAOMEO010000000
VERSION     DAOMEO010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_2219_cov_2.573937"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLH31_11800"
     CDS             <1..463
                     /locus_tag="PLH31_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="family 2 glycosyl transferase"
                     /protein_id="HQR89915.1"
                     /translation="TSLDQWGPQRARWIKGHLQTLSVHARGETRRQPRALLALIMTLG
                     VPIAASNLHAPAMLIAGVGVAVNWMADGELSVTLADLTLYMFGWSVSAACAAIGLRRS
                     GEQPRFLHLFGMVGYWMMWIFASPRAVWQFFFAPHQWDKTTHAPRTGRPTP"
     gene            481..1392
                     /locus_tag="PLH31_11805"
     CDS             481..1392
                     /locus_tag="PLH31_11805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR89916.1"
                     /translation="MSPTIAAAPLVMRTTGWSDYALLDSGNGRKLERYGPYTVVRPEP
                     QCFWSPRLPAKAWDDADAIFDPSDEDEAGRWRFKGKPIEKFPLSWGEARFHGQFTPFR
                     HLAFFPEQAANWAWQDERVRAFAKASGRQPKILNLFGYTGVASLVCAAAGAAVTHVDA
                     SKKSVGFARENAAFADLADKPIRWIVEDARKFVAREVRRGNVYDGIILDPPKFGRGAD
                     GEVWRLFEDLTELTANCAQLLSDDAAFLLMNAYAARVSGAAMSGLLAQVLEGRSGVID
                     WGELSLVEEKGDRQIGLSFFARWSSEG"
     gene            1398..2201
                     /locus_tag="PLH31_11810"
     CDS             1398..2201
                     /locus_tag="PLH31_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR89917.1"
                     /translation="MNTPNQAKTVTSLSNNTVKAVRALHMRKEREESGLFLAEGLKIV
                     IEAIDCGHAPKIIMYGRDAADHPMLQKASQACLKAGGEVIEVNREILEKVSRRDNPQA
                     VVGVFRQVFTPLDAIVPQSAPCWVAMQAVRDPGNLGTVIRTADAAGCGGVILVGDCVD
                     PYSVEGVRATMGSIFAVKIAKATIPEFLAWRETWPGSVVGTLLTATVDHKSADYVKPS
                     LILMGNEQQGLPPELAATCDVNVKIPMRGRADSLNLSVATGIMIYTVTG"
BASE COUNT          370 a          738 c          737 g          374 t
ORIGIN      
        1 cacctcgctg gatcaatggg ggccgcaacg ggcgcggtgg atcaagggac atctgcaaac
       61 gctctctgtt catgctcgcg gcgagacccg aagacagcct cgcgccctcc tggccttgat
      121 catgaccctg ggcgtgccca tcgccgcctc gaaccttcac gccccggcca tgctgatcgc
      181 cggggtgggt gtagcggtga actggatggc tgatggcgag ctgtcggtga ccctcgccga
      241 cctcaccctc tacatgttcg gctggagcgt ttcggcggcc tgcgcggcga tcgggctgag
      301 gcggtcgggc gagcagccac gtttcctgca tctcttcggc atggtcggat actggatgat
      361 gtggatcttc gcttcacccc gcgctgtctg gcagttcttt ttcgcgccgc atcagtggga
      421 caagaccacg catgcgccgc gcactggacg gccaacgccc tgaaagcgta tggcgaggcg
      481 atgagcccga cgatcgccgc cgcccccctt gtcatgcgca ccaccggctg gagcgactac
      541 gccctgctgg acagcggaaa cggcaggaag ctggaacgct atggccccta cacggtcgtg
      601 cgccccgagc cgcaatgctt ctggtcgccg cgactgccgg ccaaggcctg ggacgacgcc
      661 gacgcaatct tcgatccctc cgacgaggac gaggccggcc gctggcgctt caagggcaag
      721 cccatcgaga agttccccct gagctggggc gaggccaggt tccacggcca gttcacgccg
      781 ttccggcacc tggccttctt tcccgagcag gccgccaact gggcctggca ggacgaacgc
      841 gtgcgcgctt tcgccaaggc gtcaggtcgc cagcccaaga tcctgaatct gtttggctat
      901 acgggcgtgg cttcactggt ctgcgccgcc gccggcgcgg ccgtgaccca tgtggacgcc
      961 tccaagaagt cggtcggttt cgcccgcgag aacgccgcct tcgccgacct cgccgacaag
     1021 ccgatccgct ggatcgtcga ggacgcccgc aagttcgtgg cccgcgaggt gcggcgcggt
     1081 aacgtctatg acggcatcat tctggacccg cccaagtttg gacgcggcgc cgatggcgag
     1141 gtctggcgcc tgttcgagga cctgaccgaa ctcaccgcca attgcgcgca actgctcagc
     1201 gacgacgccg cgttcctgct gatgaacgcc tatgccgccc gggtctcggg cgcggcgatg
     1261 tcgggcctgc tggcccaggt gctggagggc cgcagcggcg tcatcgactg gggcgagctg
     1321 tccctggtcg aggaaaaggg cgaccgccag atcggcctgt cgttcttcgc gcgctggtct
     1381 tcggaagggt gaaacagatg aacaccccca accaggccaa gaccgtcacc tcgctgtcga
     1441 acaacaccgt caaggcggtc cgcgccctgc acatgcgcaa ggagcgcgag gagagcggcc
     1501 tgttcctggc cgagggcctg aagatcgtca tcgaggcgat cgactgcggc catgcgccca
     1561 agatcatcat gtatggccgt gacgccgccg accatccgat gttgcaaaaa gcctcgcagg
     1621 cctgcctgaa ggcgggcggc gaagtcattg aggtcaatcg ggaaatcctc gagaaggtct
     1681 ctcgccgcga taacccccag gccgtagtcg gggtgttccg ccaggtgttc acgccgctgg
     1741 acgccatcgt gccgcaaagc gccccgtgct gggtggcgat gcaggctgtg cgcgaccccg
     1801 gcaatctggg caccgttatc cgcaccgccg acgcggccgg ctgcggcggg gtgatcctgg
     1861 tgggcgactg cgtcgatccc tattcggtgg aaggcgtgcg ggcgaccatg ggctcgatct
     1921 tcgcggtcaa gatcgccaag gccacgatcc cggaattcct ggcctggcgc gagacctggc
     1981 ctggctcagt ggtcggcacc ctgctgaccg ccacggtcga ccacaagagc gccgactatg
     2041 tgaagccgtc gctgatcctg atgggcaatg agcaacaggg cctgccgccg gagctggcgg
     2101 cgacttgcga cgtgaacgtg aagatcccga tgcggggacg ggccgacagc ctgaacctgt
     2161 cggtagccac agggatcatg atctacacgg tgacgggtta agtccctctg ggcttggcc
//
LOCUS       DAOMEO010000532         2217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5193_length_2217_cov_2.560592, whole genome shotgun sequence.
ACCESSION   DAOMEO010000532 DAOMEO010000000
VERSION     DAOMEO010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5193_length_2217_cov_2.560592"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLH31_11815"
     CDS             complement(<1..787)
                     /locus_tag="PLH31_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR89918.1"
                     /translation="MATIQDVARDAGVSTATVSRVFSAPELVLEATRSKVMEAVNRLG
                     YEPNFAARSLRTLRTEKILVTVPDISNPFFSQVIRGVEEAAMAAGYSVLLGDTRHEEA
                     REEQYAAMFRRKEADGLIFLGHRLPNVLADMVAAKGPRTPIVNGCEFRPGLAVSSAHI
                     DNERAAAEVMTYLYGLGHTRIGVVTGPLASPISSDRLTGVLAAAQRQGRASALRISVG
                     DFSIESGVRQTGELLDEPGRPSAIFCFSDEMAMGALEVIRQRGL"
     gene            966..>2217
                     /locus_tag="PLH31_11820"
     CDS             966..>2217
                     /locus_tag="PLH31_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR89919.1"
                     /translation="MTTSFRLFLMMVLQLAIWGAWAPKIFPYMGMLGFEPWQQSLVGS
                     SWGVAALVGIFFSNQFADRNFAAERFLAVSHLIGGLALVGTAFATSFWPFFACYLIFS
                     LVYVPTLSVTNSIAFANLRNPAADFGAVRMGGTVGWVLVSWPFVFLLGANADVEQMRW
                     IFLVAALVSFVFAAYSLTLPHTPARKDAPGIDKLAWRRAFKLLATPFVLVLFIVTFID
                     SVIHNGYFVMADAFLTNRVGIAGNLSMVVLSLGQVAEILTMFLLGRVLAKLGWKITMI
                     VGVLGHAARFAVFAFFADSIPVIVAVQLLHGICYAFFFATVYIFVDAVFPKDVRSSAQ
                     GLFNLLILGVGNVVASFLFPSLISRLTQTGADGVATVDYTSLFLVPTGMALAAVCLLA
                     LFFKPPTRAPVAEVADDGPVAAATP"
BASE COUNT          306 a          740 c          742 g          429 t
ORIGIN      
        1 gcaggccgcg ctggcggatg acctccagcg cgcccatggc catctcgtcg ctgaagcaga
       61 agatcgccga cggccggccg ggctcgtcca gaagctcgcc cgtctggcgc acgcccgact
      121 cgatcgagaa atcgccgacg gagatgcgca gggcgctggc gcggccctga cgctgggcgg
      181 cggccagcac gccggtcagg cggtcgctgc tgatcgggct ggccagaggg ccggtgacca
      241 cgccgatgcg ggtgtggccc aggccgtaga gatacgtcat cacctcggcg gcggcgcgtt
      301 cgttgtcgat gtgggcgctg gagaccgcca ggcccggccg aaactcgcag ccgttgacga
      361 tcggcgtccg ggggcccttg gcggcgacca tgtcggccag aacgttcggc aggcggtggc
      421 ccaggaagat cagaccgtcg gcctccttgc gccggaacat ggcggcgtac tgctcttcgc
      481 gcgcctcctc gtggcgggtg tcgcccagca gaaccgaata gcccgccgcc atggcggctt
      541 cctcgacgcc ccggatcacc tgggagaaga aggggttgga gatgtccggc acggtgacca
      601 ggatcttttc ggtccgcagg gtgcgcaggc tcctggccgc gaaattgggc tcgtagccca
      661 ggcggttgac cgcctccatc accttgctgc gcgtcgcttc tagcaccagt tcgggagcgc
      721 tgaacacgcg ggaaaccgtc gcggtcgaga cacccgcgtc ccgcgcgaca tcttgaatgg
      781 tggccatcgg cggacactgg cgacaaggcg ggagcttggc aaggcacagc gaggcgcggc
      841 tggttgatcg gttacacggt tgccgccttg acgggccttg ctatcgatga tttcgattac
      901 atcagagcgg atgcggtcca aacgggtcga aacaagccgc ctgtgctggt cggggagggc
      961 caaccatgac gacgagcttc cggctctttc tgatgatggt gctgcaactg gccatctggg
     1021 gcgcctgggc gcccaagatc ttcccctaca tgggcatgct gggcttcgag ccgtggcagc
     1081 agtcgctggt cggcagctcg tggggcgtgg ccgccctggt cggcatcttc ttctccaacc
     1141 agttcgcgga ccgcaatttc gcggccgagc gcttcctggc cgtcagccac ctgatcggcg
     1201 gtctggcctt ggtcggcacg gccttcgcga ccagcttctg gcctttcttc gcctgctacc
     1261 tgatcttcag cctggtttat gtgccgaccc tgtcggtgac caactccatc gccttcgcca
     1321 acctgcgcaa cccggcggcc gacttcgggg cggtgcgcat gggcggcacg gtgggctggg
     1381 tgctggtcag ctggccgttc gtcttcctgc tgggcgccaa tgccgacgtc gagcagatgc
     1441 gctggatctt cctggtggcg gcgctcgtct ctttcgtctt cgctgcctac tctctgacct
     1501 tgccgcacac tccagcccgc aaggacgcgc ccggcatcga caagctggcc tggcgacgcg
     1561 ccttcaagct gttggcgacg ccgttcgtgc tggtgctgtt catcgtcacc ttcatcgatt
     1621 ccgtcatcca caacggctac ttcgtgatgg ccgacgcctt cctgaccaac cgtgtgggca
     1681 tcgccggcaa tctgagcatg gtggtgctga gcctgggcca ggtggccgag atcctgacca
     1741 tgttcctgct gggccgggtg ctggccaagc tgggctggaa gatcaccatg attgtcgggg
     1801 tgctgggcca cgccgcgcgc tttgcggtct tcgccttctt cgccgacagc atcccggtga
     1861 tcgtggccgt gcagctgctg cacgggatct gctacgcctt cttcttcgcc acggtgtaca
     1921 tcttcgtcga cgcggtgttt cccaaggatg tgcgctccag cgctcagggc ctgttcaacc
     1981 tgctgatcct gggcgtcggc aatgtggtgg ccagcttcct gttcccgtcg ctgatcagcc
     2041 gcctgaccca gacgggcgcc gatggcgtcg cgacggtgga ctataccagc ctgttcctgg
     2101 tccccaccgg catggctctc gcggccgtgt gtctgttggc cctgttcttc aaaccgccga
     2161 cccgcgcccc ggtggccgag gtcgccgacg acgggccggt ggcggccgcc accccgg
//
LOCUS       DAOMEO010000533         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5197_length_2216_cov_2.119389, whole genome shotgun sequence.
ACCESSION   DAOMEO010000533 DAOMEO010000000
VERSION     DAOMEO010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_2216_cov_2.119389"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLH31_11825"
     CDS             <1..680
                     /locus_tag="PLH31_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQR89920.1"
                     /translation="QILCLQRGAYMTSLGGYGLRIDKRNDPIQPTRGWFADLNQDLAG
                     FGGDVKYLKTETDAGWYWGFNKDFIFSATGSAGYIEGWGGDNVRINDRFYKGGTNFRG
                     FETAGIGPRDVSTQSNSLGGKLYAIGTFELTVPTLLPEQYGIKAAVFSDFGTAGLLDD
                     VDRQSSAGVFDPNIRDNLGLRASAGISIDWKSPMGPIRFDFSKILSKEDYDRTETFRF
                     STSTRFQ"
     gene            683..1312
                     /locus_tag="PLH31_11830"
     CDS             683..1312
                     /locus_tag="PLH31_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQR89921.1"
                     /translation="MTSKFLFAAASGVVAIAMSTSAVAQTAPAAPAISHGAPITGVCI
                     FSSQRAVGSSAVGKAVDARLKTIIAQVNAELTGERTTLDNEAKALDAKKATMDQGALE
                     QQAAGLQVKANAWQRKGQLRQKEVEATEQKALSRVYQELDPAIKQVYQQRTCGILLDR
                     ESVLLANPAMDITDAVVVALDARIKTLTFDRERLDQPTAGAQALTPTRK"
     gene            1361..>2216
                     /gene="lpxD"
                     /locus_tag="PLH31_11835"
     CDS             1361..>2216
                     /gene="lpxD"
                     /locus_tag="PLH31_11835"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQR89922.1"
                     /translation="MPDPRFFDDMGPVSLDELSRLGVAELADASLASRSITHVAPLDA
                     ADGRAVTFFSDAKRRDAAGDTRAGACFVRPEHRELLAPDCAALLTAHPQAAWAAAAAR
                     LHAPRRHEPLSPSIHPDCDLEDGVRLSPGVTVGQGARIGRGTWLSPGCVIGPGVSIGR
                     DCLIGANAVIGFAIIGDRVSIHAGAVIGEAGFGAAGGPRGVVDLPQLGRVVIQDGVTI
                     GANSCVDRGAFADTMIGENTKIDNLVHVAHNVRLGRNCVLAAYTGLSGSTVVGDGVAF
                     GGKAGVADH"
BASE COUNT          381 a          737 c          715 g          383 t
ORIGIN      
        1 cccagatcct gtgcctgcag cgcggcgcct acatgacgtc cctgggcggc tacggcctgc
       61 ggatcgacaa gcgcaacgat ccgatccagc ccactcgcgg ctggttcgcc gacctgaacc
      121 aggacctggc gggcttcggc ggcgacgtga agtacctgaa gaccgagacc gacgcgggtt
      181 ggtactgggg cttcaacaag gacttcatct tcagcgccac cggttcggcc ggctatatcg
      241 agggctgggg cggcgacaac gtccgtatca atgatcgctt ctacaagggc ggcaccaact
      301 tccgcggctt cgagactgcc ggtatcggtc cgcgcgacgt atcgacccag agcaactcac
      361 tgggcggcaa gctctatgcc atcggcacct tcgagctgac ggttccgacg ctgctacccg
      421 agcagtacgg catcaaggcc gccgtgttca gcgactttgg tacggccggc ctgctcgacg
      481 acgtcgaccg ccagtcctcg gccggcgtgt tcgatcccaa tatccgcgac aacctgggtc
      541 tccgggcgtc ggcgggtatc agcatcgact ggaagtcgcc catgggtccg atccggttcg
      601 acttcagcaa gatcctttcc aaggaagact atgaccggac agaaacgttc cggttctcca
      661 cctccacaag gttccaataa tcatgacttc caagttcctg ttcgcagccg cgtccggcgt
      721 cgtcgccatt gccatgtcga ccagcgctgt cgcccagacg gctccggccg ccccggcgat
      781 ctcgcacggc gcgcccatca ctggcgtttg catcttctcg agccagcgcg ccgtcggctc
      841 gtcggctgtc ggcaaggccg tcgacgcccg cctgaagacc atcatcgcgc aagtgaacgc
      901 cgagctgacc ggcgaacgca ctacgctcga caacgaagcc aaggcgctgg acgccaaaaa
      961 ggcgacgatg gatcaaggcg cgcttgagca gcaggccgct ggtctgcagg tcaaggccaa
     1021 cgcctggcag cgcaagggcc agctgcgtca gaaggaagtt gaagccaccg agcagaaagc
     1081 cctgtcgcgc gtctatcaag agctcgatcc ggctatcaag caggtctatc aacagcgtac
     1141 ctgcgggatt ctgctggacc gcgagtcggt gcttctggcc aaccccgcca tggatatcac
     1201 cgacgccgtc gtcgtggcgc ttgacgctcg catcaagacc ctgaccttcg atcgcgaacg
     1261 tctggaccaa cccaccgccg gcgctcaagc tctgacgccg accaggaagt aagcgagtcg
     1321 gcccgaacct cacgctcggg ctgatcgtcg agaggacgag atgccggacc cacgattttt
     1381 cgatgacatg ggtccggttt cactcgatga gctctcccgc cttggcgtag cagagcttgc
     1441 ggacgccagc ctggcgtcgc ggtccatcac ccatgtcgcg ccgcttgacg ccgctgacgg
     1501 acgggcggtc accttctttt ccgacgccaa gcgccgcgac gcagcaggcg acacccgggc
     1561 aggcgcctgc tttgttcgtc cggaacaccg tgagctgctc gcacccgatt gcgcggcgct
     1621 gctgacggcg caccctcagg ccgcctgggc cgccgcggcg gcgcggttgc atgcgccgcg
     1681 ccgccacgag ccgctatcgc cttcaatcca tcccgattgc gacctggagg acggcgtgag
     1741 gctgtcgccc ggcgtcaccg tggggcaggg ggcgaggatc gggcgtggaa catggttgtc
     1801 gcctggctgt gtgatcggtc ccggggtcag catcggtcgt gactgcctga tcggcgccaa
     1861 cgcggtgatc ggctttgcga tcatcggcga ccgggtcagc atccatgcgg gcgcggtgat
     1921 cggcgaggcg ggctttggcg cggccggcgg gccgcgtggg gtggtggatc ttccccagct
     1981 gggccgggtg gtaatccagg acggtgtcac catcggcgcc aacagctgcg tcgaccgggg
     2041 cgcctttgcc gatacgatga tcggcgagaa caccaagatc gacaatctgg ttcacgtcgc
     2101 gcacaacgtc cgtctgggtc gcaattgcgt gctggcggcc tatacagggc tgtccggcag
     2161 cacggttgtc ggcgacgggg tggcgtttgg cggcaaggcc ggcgtcgccg atcatt
//
LOCUS       DAOMEO010000534         2215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5202_length_2215_cov_1.644444, whole genome shotgun sequence.
ACCESSION   DAOMEO010000534 DAOMEO010000000
VERSION     DAOMEO010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_2215_cov_1.644444"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1379)
                     /locus_tag="PLH31_11840"
     CDS             complement(234..1379)
                     /locus_tag="PLH31_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR89923.1"
                     /translation="MDFNFTEEQSMVRDTVASFLQDKYDFETRRKIVASESGWRADYW
                     KAFAEELGILGAPFSEELGGLGGGAIDNMIIMEEFGKALVIEPYLGTVVIGGGFMKHS
                     GYAHAASVIEGIVGGTTTIAFAYAEPQGRYTWRDLKTTAKKDGSGYILNGHKAVVVGA
                     PFASHLIVTARTGGAQRDAGGVSVFLVDKSLPGIVTRDYPTVDGNRASEVYFENVSIP
                     ADALIGGEGEGLPLVDKVIDEATAAVGAEAVGVLRKLHENTLDYAKQRKQFGTAIANF
                     QVLQHRMVDMFINVEQSVSMAYMATIKIDENDATRAPAISAMKVQIGRACKFVGQNAI
                     QIHGGMGMTDELAIGHYFKRASIIEGQFGSVDHHLKRYESLSFAAAA"
     gene            complement(1504..>2215)
                     /locus_tag="PLH31_11845"
     CDS             complement(1504..>2215)
                     /locus_tag="PLH31_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR89924.1"
                     /translation="QHADWIFCLVRTDPTAKIQEGISFLLIDMKSPGVTVRPLITLGG
                     EHEVNEVWLENVKVPVENRIFDENKGWTCAKFLLAHERSGIAGVARSKRGIERIRSIA
                     AAELSDDGESLLADPVFKRKVAELEIDLTALEFTELRTLAGEASGKGPGPESSVLKIK
                     GTEIQQRITELVLEAAGHYGAPYFRGFPNDGDNAHPIGPDFSHRAAPTYFNVRKTSIY
                     GGSNEIQRNIIAKMVLGL"
BASE COUNT          369 a          694 c          720 g          432 t
ORIGIN      
        1 aggcggatca gttcagcgca cggaacatcc ccttctggca cgttggactg cacccttaat
       61 ttgctccgta caaggcgcat ctagccggga tcgccgtcca ggcctgtcag gcggccagcg
      121 atctttctcg acccttccaa aacgataaac cgcccgcgtc agcgtgacgc gggcggcttt
      181 caagacttcg gtccttgctc cgtgggcgtc accaccccga agcggccagc accttaagcg
      241 gcggcggcaa aagacaggga ttcgtagcgc ttgagatggt gatcgaccga gccaaactga
      301 ccctcgatga tcgaggcgcg cttgaagtaa tggccgatgg ccagctcgtc ggtcatgccc
      361 atgccgccat ggatctgaat ggcgttctgg ccgacgaact tgcaggcccg gccaatctgc
      421 accttcatgg ccgagatcgc cggggcgcgg gtggcgtcgt tctcatcgat cttgatcgtc
      481 gccatatagg ccatcgacac cgactgctcg acattgatga acatgtcgac catgcggtgc
      541 tgcagcacct ggaagttggc gatggcggtg ccgaactgct tgcgctgctt ggcgtagtcg
      601 agggtgttct cgtgcagctt gcgcagcacg ccgaccgctt cggcgccaac ggcggccgtg
      661 gcctcgtcga tcaccttgtc caccagcggc aggccttcgc cctcgccgcc gatcagggcg
      721 tcggccggga tcgagacgtt ttcgaaatag acttccgagg cgcggttgcc gtccacggtg
      781 ggatagtcgc gggtgacgat gcccggcagg ctcttgtcga ccaggaacac cgacacgccg
      841 ccggcgtcgc gctgggcgcc gccagtgcgg gccgtgacga tcaggtggct ggcgaacggc
      901 gcgccgacca cgacggcctt atggccgttg aggatgtagc ccgagccgtc cttcttggcg
      961 gtggtcttca ggtcgcgcca ggtatagcgg ccctgaggct cggcataggc gaaggcgatc
     1021 gtggtggttc cgccgacaat gccttcgatg accgaggccg cgtgggcgta gccggaatgc
     1081 ttcatgaacc cgccgccgat caccaccgtg cccagatagg gctcgatgac cagagctttg
     1141 ccgaactctt ccatgatgat catgttgtcg atggcgccgc cgcccagacc gcccagttct
     1201 tcgctgaacg gcgcacccag gatgcccagt tcctcggcga aggccttcca gtagtcggcg
     1261 cgccagccgc tttcggaggc cacgatcttg cgacgggtct cgaagtcgta cttgtcctgc
     1321 aggaagctcg cgaccgtgtc gcggaccatt gactgctctt cggtgaagtt gaagtccatc
     1381 ccagggtttc ccgtcttttt cttttgtcat cccggtcggc gcttaggaga gccgggatca
     1441 tcaaggtcgt gtgcgaaagc cccggaccgc cgcagcggtc cggggaaaac cgtcattgtg
     1501 gcgctagagc cccagaacca tcttggcgat gatattgcgc tggatctcgt tggagccgcc
     1561 atagatcgac gtcttgcgga cgttgaaata ggtgggcgcc gcgcgatggc tgaagtccgg
     1621 gccgatcgga tgggcgttgt cgccatcgtt cgggaagccc cggaaatagg gcgcgccgta
     1681 gtggcccgcg gcttccagaa ccagttcggt gatccgctgc tggatctcgg tgcccttgat
     1741 cttcaggacg ctggattccg ggcccggtcc cttgccggag gcctcgcccg ccagggtccg
     1801 cagttcggtg aactccagag cggtcagatc gatctcgagt tcggcaacct tgcgcttgaa
     1861 gaccggatcg gccagcaggc tttcgccgtc atcggacagc tcggcggccg caatgctgcg
     1921 gatccgttcg atgccacgct tggagcgcgc aacgccggca atgcccgaac gctcgtgggc
     1981 cagcaggaac ttggcgcagg tccagccctt gttctcgtcg aagatgcggt tttcgaccgg
     2041 caccttgacg ttctcgagcc agacctcgtt gacctcgtgc tcgccgccga gcgtgatgag
     2101 cgggcgcacg gtgacgccgg gcgacttcat gtcgatcagc aggaacgaga tgccttcctg
     2161 gatcttggcg gtcgggtcgg tgcggaccag gcagaagatc cagtcggcgt gctgg
//
LOCUS       DAOMEO010000535         2214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5203_length_2214_cov_3.220009, whole genome shotgun sequence.
ACCESSION   DAOMEO010000535 DAOMEO010000000
VERSION     DAOMEO010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_2214_cov_3.220009"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..450
                     /locus_tag="PLH31_11850"
     CDS             46..450
                     /locus_tag="PLH31_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR89925.1"
                     /translation="MQPHSWVAIVTLVALLVYVWMGVRVGGARKKSGIAAPAMTGDPE
                     LERHIRVQANTLEWLPIFLPSLWLFAIYWSDLIATIAGAVWIVGRILYALGYAAAADK
                     REVGFVIQMLATAVLLFGALGRAIWMLVTVGA"
     gene            493..1122
                     /locus_tag="PLH31_11855"
     CDS             493..1122
                     /locus_tag="PLH31_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR89926.1"
                     /translation="MALPVDPARYGAFLAAMFVMAITPGPANLFAIATGMEKGKGAAL
                     LGVVGMNSATLIWFAGSALGLGALIIAFPQAFHILALLGAAYLVWLGLKSLWAGWKNA
                     ESHAVATVKGGKSAFLDGFTVQIANPKALLFFTAVLPPFIDVNRPLAPQLLMFAIATI
                     GMDLISMSAYGLGGAALSTRMNEPVFRRVFAFVVGALLICAAILIVSRG"
     gene            1235..1828
                     /locus_tag="PLH31_11860"
     CDS             1235..1828
                     /locus_tag="PLH31_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89927.1"
                     /translation="MSLKKAALVAAMVVAAGLSACATATPYQPKTTSGSVTGGFSELR
                     LEADRYRVSFAGNSLTSRETVERYLLYRAAELTVQQGYDWFETADRRTDRTARTVIDP
                     DPLARPGFGYGYPYGYWRPSWRYFGPSYGWRSWDPFWGDPFFYDRTQVRTIERFEAGA
                     EIVMRKGKKPEGDPRAYDAREIMTNLASSIVLPTPKK"
     gene            complement(1957..>2214)
                     /locus_tag="PLH31_11865"
     CDS             complement(1957..>2214)
                     /locus_tag="PLH31_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR89928.1"
                     /translation="MTLPIARDLMSEGIRVNCILPGIFNTPLMNNAPEAVKAGLAASV
                     PFPKRLGNPEEYAHLALTMITNDYFNGEDVRLDGGIRMAPR"
BASE COUNT          343 a          745 c          709 g          417 t
ORIGIN      
        1 gtccgcttca aggccccctc cgtgggccca tggggagaaa cacatatgca gccgcattcc
       61 tgggtcgcta tcgtcacgct agtcgccctg ctggtttatg tctggatggg ggttcgggtt
      121 ggcggggccc gcaagaagag cggcatcgcc gcgccggcca tgacgggcga tcccgagctg
      181 gagcgccaca tccgcgttca ggccaatacg ctggaatggc tgccgatctt cctgccgtcg
      241 ctgtggctgt tcgccatcta ctggagcgac ctaatcgcca ccatcgccgg cgcggtctgg
      301 atcgtgggcc gaatcctcta tgccctgggc tacgccgccg cggcggacaa gcgcgaggtc
      361 ggcttcgtca tccagatgct ggcgacagcg gtcttgctgt ttggcgccct gggacgggcg
      421 atctggatgc tggtgaccgt cggcgcctag acagcgcttt tcgacgggtg ggagctcggc
      481 tatggcgtgg ccatggctct ccccgtcgac cccgcccgct atggcgcctt cctggccgcc
      541 atgttcgtca tggccatcac gccgggtccc gccaacctgt tcgccatcgc cacgggcatg
      601 gaaaagggca agggcgcggc cctgctgggc gtggtgggca tgaacagcgc caccctgatc
      661 tggttcgccg gttcggccct gggcctgggc gccctgatca tcgccttccc gcaggccttt
      721 cacatcctgg cgctgctggg cgcagcctat ctggtctggc tgggcctgaa gtcgctgtgg
      781 gcgggctgga agaacgccga gagccatgcg gtcgccaccg tcaagggcgg caagtcggcc
      841 tttctcgacg gcttcaccgt ccagatcgcc aatcccaagg ccctgctgtt cttcaccgcc
      901 gtgctgccgc ccttcatcga cgtcaaccgg cccctcgcgc cgcagctgct gatgttcgcc
      961 atcgccacca tcggcatgga cctgatcagc atgagcgcct acggcctggg tggagcggcc
     1021 ctatcgaccc gaatgaacga gcccgtcttc aggcgagtct tcgccttcgt ggtcggggcc
     1081 ttgctgatct gcgcggcgat cctgatcgtt tcgcgcggct gacgtctgag tcacgctgat
     1141 ttcaggcggg acatcaaacg ccttggcgaa accacaaacc agcgccagag ttaaagccca
     1201 gacccatcgg gttccgggtc acggagcgtc aaacatgtcc ctgaagaaag ccgccctcgt
     1261 cgcggccatg gtcgtcgccg ccggactgtc ggcctgcgcg accgccaccc cctaccagcc
     1321 caagaccacg tcaggttcgg tcaccggcgg tttctcggaa ctgcgtctcg aggccgaccg
     1381 ctatcgcgtc agcttcgccg gcaacagcct gacctcgcgc gagaccgtcg agcgctatct
     1441 gctgtaccgc gccgccgagc ttaccgtgca gcagggctat gactggttcg agaccgccga
     1501 ccgccgcacc gaccgcacgg cccgcacggt gatcgatccc gatcccctgg cgcgcccggg
     1561 ctttggctat ggctatcctt atggatactg gcgcccgtcc tggcgctatt tcggccccag
     1621 ctatggctgg cgctcatggg accccttctg gggtgacccc ttcttctatg accgcaccca
     1681 ggtgcggacc atagagcggt tcgaggccgg cgccgagatc gtgatgcgca aaggcaagaa
     1741 gcccgagggt gatccgcgcg cctatgacgc ccgcgagatc atgaccaacc tggccagcag
     1801 catcgttctg ccaacgccga agaagtagag aaagctgctc cccttttggg ggagctgtcg
     1861 cggagcgact gagggggctg ctcggcttat gccgcgctag ccccctcagg ccctctgggc
     1921 cacctccccc agagggggag gatcatcttg acgtgtttag cgcggcgcca tgcggatgcc
     1981 gccgtcgagg cgcacgtctt caccattgaa atagtcgttg gtgatcatcg tcagggccag
     2041 gtgagcatat tcctcggggt tgcccagacg cttggggaac ggcaccgagg cggccaggcc
     2101 ggccttcacg gcttcgggcg cattgttcat cagcggggtg ttgaagatac ccggcaggat
     2161 gcagttgacg cggatgcctt cgctcatcag gtcgcgtgcg atcggcaggg tcat
//
LOCUS       DAOMEO010000536         2212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5210_length_2212_cov_2.485860, whole genome shotgun sequence.
ACCESSION   DAOMEO010000536 DAOMEO010000000
VERSION     DAOMEO010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5210_length_2212_cov_2.485860"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..744
                     /locus_tag="PLH31_11870"
     CDS             82..744
                     /locus_tag="PLH31_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR89929.1"
                     /translation="MHIEKFVHSCLRLTVGADALLFDPGKFGFIDGRVDPKTLAGANV
                     VVITHAHPDHLDPAALKTILAGGGQVVGNAEVRDKLADEGLAVEVFDEGVRRFGAFEL
                     RAIPVPHEPILAKDTPRVTAFLVNGRVLNCGDSFDDALLPHAGVEALALPVMAPYLTE
                     LKVMAFAKAMRPKFALPLHDGHARDFFVEQRYDTYEPFFREAGIEFVRLAKPGDAFDL
                     AE"
     gene            complement(752..2182)
                     /locus_tag="PLH31_11875"
     CDS             complement(752..2182)
                     /locus_tag="PLH31_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQR89930.1"
                     /translation="MTRSLAFILLLASAAPAWAGQAPGPASAPDIAVSNRDRIYTSDQ
                     FSNTVSVIDPAANKLLGVIKLGDQTPMNLSPLYKGQLLVHGMGFSPDHKTLAVISIGS
                     NSVTFIDTATNAVKHTAYVGRSPHEAFFTPDGKEVWVAVRGEDYLQVLDGATYKETAR
                     IKVPNGPGMTIFAPDGRYAYVCSSFTPETVVIDTKTKAIVGRVKQESPFCPDIAATPD
                     GKQVWLTLKDIGKVMVFDAKPPFAVLKTLDTGPITNHVNTVRNGAGQFAYVTVGTQNA
                     VKVFRTDSFAQVAEIKVGALPHGLWPSGDGSRVYVGLENGDAVAAIDTATNTVIATIP
                     IGQAPQGVAYVPNAVPTGPGTANLQPLGLAAKSDSLTLAGAAGGPPTQVTLFDQGQVQ
                     ILQAAAVGLKPKAAYVLAIADNPKGEGKLQALAGFMTSPSGSAVVNAVGPLRNVVSAD
                     TPAPRRYLVIAEGKPDELGIVVQTQR"
BASE COUNT          357 a          718 c          726 g          411 t
ORIGIN      
        1 gcaagccgca cccgccaaga cattagtcga ggaactgcag gggcgtgggc gccttgagtc
       61 tcggcctcca ggaagcacgc catgcacatt gaaaagttcg tccactcctg cctgcggctg
      121 accgtcggtg cagacgccct gctcttcgac ccgggcaagt ttgggttcat cgacggccgg
      181 gtcgatccca agacactggc gggcgccaac gtcgtggtga tcacccacgc tcacccggat
      241 cacctcgatc cggcggccct caagaccatc ctggccggcg gcggacaggt cgtcggaaac
      301 gctgaggttc gcgataagct ggccgacgaa ggcctcgcgg tcgaggtgtt cgacgaaggt
      361 gtccgccggt tcggggcctt cgagttgcgc gccatccctg tccctcacga accgatcctg
      421 gccaaggata cgccgcgggt aaccgccttc ctggtcaacg gccgcgtgct caattgcggc
      481 gactccttcg acgatgcgct gctcccccat gccggcgtcg aggcgctcgc cctgcccgtc
      541 atggccccat acctgacgga gctgaaggtg atggccttcg ctaaggccat gcgacccaag
      601 ttcgccctgc ccctccacga cggccatgcc cgcgatttct tcgtggagca gcgctacgac
      661 acctacgagc ccttcttccg tgaagccggc atagaattcg tgcggctggc taaacctggc
      721 gacgccttcg atctagctga gtgacaggcc gctagcgctg ggtctgcacc acaatcccga
      781 gttcatcggg cttgccttcg gcgatcacca gatagcggcg cggcgccggc gtgtcggcgc
      841 tgaccacatt gcgtagcggt cccaccgcgt tgaccacggc cgaccccgat gggcttgtca
      901 tgaagccggc cagggcctgc agctttccct cgcccttggg attgtcggcg atcgccagca
      961 cataggccgc cttcggcttt agacccacgg ccgccgcctg caggatctgc acttggccct
     1021 ggtcgaacaa tgtcacctgg gtgggtggtc cgcccgcggc gccggcgaga gtcaggctat
     1081 cggatttagc ggccaagccg agcggctgga ggttggccgt acccgggcca gtgggaacag
     1141 cgttggggac ataggccacg ccctgaggcg cctggccgat cgggatggtg gcgatcacgg
     1201 tgttggtcgc cgtatcgatg gccgccaccg cgtcgccatt ctccaggccg acatagacgc
     1261 gcgagccgtc accggacggc cataggccat gcggcagcgc gccgaccttg atctcggcca
     1321 cctgggcgaa gctgtccgtc cggaacactt tcaccgcgtt ctgcgtgccg acggtgacat
     1381 aggcgaactg gccggcgcca ttgcgcacgg tgttgacgtg gttggtgatc gggccggtgt
     1441 cgagcgtctt cagaaccgcg aacggcggct tggcgtcgaa caccatcacc ttgccgatgt
     1501 cctttagcgt caaccagacc tgcttgccgt ccggcgtggc ggcgatgtcc gggcagaacg
     1561 ggctttcttg tttcacccgg ccgacgatcg ccttggtctt ggtgtcgatc accacggtct
     1621 ccggggtgaa gctcgagcac acgtaggcat accggccatc gggggcgaag atagtcatgc
     1681 ccgggccgtt gggcactttg atccgcgctg tctccttgta ggtcgcgccg tcgagcacct
     1741 gcagatagtc ctcaccgcgc accgcgaccc agacttcctt gccgtcgggg gtgaagaagg
     1801 cctcgtgcgg cgagcggccg acatatgccg tgtgcttcac cgcgttggtc gctgtgtcga
     1861 tgaaggtcac tgaattggag ccgatggaga tcaccgccaa ggtcttgtgg tccggcgaga
     1921 aacccatccc atggaccagc aactggccct tgtagagcgg gctcaaattc atgggcgtct
     1981 ggtcgcctag cttaatgacg ccgagcagct tgttagcggc cgggtcgatc accgagacgg
     2041 tgtttgagaa ctggtccgac gtatagatgc ggtcgcggtt gctcaccgca atgtcgggcg
     2101 cgcttgcggg tccaggcgct tgcccggccc aagccggcgc ggcgctggcc agcaacagga
     2161 tgaaggcgag actgcgggtc attgcgggtg tccttgcgga tgggtggcgg gg
//
LOCUS       DAOMEO010000537         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5225_length_2206_cov_2.592748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000537 DAOMEO010000000
VERSION     DAOMEO010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5225_length_2206_cov_2.592748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PLH31_11880"
     CDS             complement(<1..1219)
                     /locus_tag="PLH31_11880"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HQR89931.1"
                     /translation="MTDTQIPEATVAWCESALLADGWARSVRLTLSEGRIARIDTDVP
                     AGDALRLGAALPGLSNVHSHAFQRAMAGLAEARGETGDNFWTWREVMYRFLGRLTPDD
                     VQTIAAMGQIEMLEGGFTRVGEFHYLHHDPSGGSYDDPAEMAVRIAAAADETGIGLTL
                     LPVFYAHSNFGGAPPTDGQKRFIHDVDGFARLVERCREIVSATPDAVVGIAPHSLRAV
                     TAEELDAILPLAGDGPIHMHVAEQTKEVDDCLAATGQRPVRWLMNHTPVDRRWCLIHA
                     THINAMETERLAKSGAVAGLCPVTEANLGDGVFPTHDYLAAGGAFGLGTDSNVLIDAA
                     EEIRALEYAQRLTRRARNVLAGGPRRSTGGQLWRGACAGGAQALGAGRGGLKRGAAAD
                     FITLDREHPALIGR"
     gene            1293..1982
                     /locus_tag="PLH31_11885"
                     /pseudo
     CDS             1293..1982
                     /locus_tag="PLH31_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
BASE COUNT          344 a          766 c          734 g          362 t
ORIGIN      
        1 cgcggccgat cagggccggg tgctcgcgat ccagggtgat gaagtcggcc gccgcgccgc
       61 gcttcaggcc gcctcgcccc gcgccaagcg cctgggcccc gcccgcgcag gcgccgcgcc
      121 acagctggcc gccggtcgag cgccggggtc cgccggccag cacgttgcga gcccggcggg
      181 tcaggcgctg ggcatattcc agggcgcgga tttcctcggc cgcgtcgatc aggacgttgg
      241 agtcggtgcc caggccaaac gccccgccgg ccgccagata gtcatgggtc gggaagacgc
      301 cgtcgcccag attggcctcg gtgaccgggc acaggcccgc cacggcgccg ctcttggcca
      361 ggcgctcggt ctccatggcg ttgatatggg tagcgtggat caggcaccag cgcctgtcga
      421 ccggggtatg gttcatcagc cagcgaaccg ggcgctggcc ggtggcggcc aggcagtcgt
      481 cgacctcttt ggtctgctcg gccacgtgca tgtggatcgg gccgtcgccg gccaggggca
      541 ggatggcgtc cagctcctcg gcggtgaccg cccgcaggct gtggggcgcg atgccgacca
      601 ccgcgtcggg cgttgcggag acgatctctc ggcagcgctc caccagacgc gcgaagccgt
      661 cgacgtcgtg gatgaagcgc ttctggccgt cggtgggcgg cgcgccgccg aagttggagt
      721 gggcgtagaa caccggcagc aaggtcaggc cgatgcccgt ctcgtccgcc gccgccgcga
      781 tccgcacggc catctcggcg gggtcatcgt acgagccgcc cgatgggtcg tggtgcagat
      841 agtggaactc gcccacgcgg gtgaagccgc cctccagcat ctcgatctgg cccatggcgg
      901 cgatggtctg gacgtcgtcg ggggtgaggc gacccaggaa gcggtacatc acctcgcgcc
      961 aggtccagaa gttgtcgccc gtctcgcccc gtgcctcggc caggcccgcc atggcccgct
     1021 ggaaggcgtg gctgtggacg ttggagaggc ccggcaaagc tgctcccaag cgaagcgcgt
     1081 caccggccgg gacgtcggtg tcgatgcggg cgatgcggcc ctccgacagg gtcagtctga
     1141 cgctccgcgc ccagccgtcg gccagcagcg cgctctcgca ccacgcaacg gtggcttcag
     1201 gaatctgcgt gtcggtcact ggacagggct cctcgcttgc caatatgtct atacatattg
     1261 aaggcggccg caagagaggg agggcgggcg aaatgcgttg cgacaccctg tggataagtt
     1321 gccgtctcgc caccctcgat ccgacccgcg agggcctggg aatcgtcgag cgcggcgtgg
     1381 tcgccgcccg cgacggccgc atcgtctatg ccggcgaggc cgtcgaggcg ccggccttcg
     1441 aggccgacca gaccatcaac tgcgaaggcc gctggatcac ccccggcctg atcgacccgc
     1501 acacccatct ggtcttcgcc ggcgaccgcg cccacgagtt cgagctgcgg ctggccggcg
     1561 cctcctatga ggaaatcgcc cgggccggcg gcggcatcgt ctcgaccatg accgccaccc
     1621 gcgcggcctc ggaagacgat ctggtcctcg ccgccctgcc ccgtctggac gccctgatcg
     1681 ccgagggtgt caccacggtc gagatcaagt ccggctacgg cctgtcgctg gacgacgagc
     1741 tgaagtgcct tcgcgccgcg cgcgccctgg ccgcctatcg ctcggtcgac gtccgcacca
     1801 ccctgctggg cgcccacgcc ctgccgcccg agttcaaggg caatccggac ggctatatcg
     1861 acctcgtctg caagcagatg atccccgccg tcgccgacgc caagctggcc gacgcggtcg
     1921 acgtcttctg cgagggcatc ggcttcaccc ccgcccagac ccgccgcgtg ttcgaggccg
     1981 ctcaccggcg aggcctggaa cacctttttg agatcttcat gttcagtatc aattccacca
     2041 tcaataactg ccacaataat tggagatgat cttttacctt tcagaatttc cttataagcc
     2101 ctttctgtac tgataccaaa aacaccgtca gattttaagt cgagattttg ccagtttgcg
     2161 acaggagatt catcctccgc actttgggca aatgagataa gaggta
//
LOCUS       DAOMEO010000538         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5226_length_2205_cov_2.745581, whole genome shotgun sequence.
ACCESSION   DAOMEO010000538 DAOMEO010000000
VERSION     DAOMEO010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_2205_cov_2.745581"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLH31_11890"
     CDS             complement(<1..612)
                     /locus_tag="PLH31_11890"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR89932.1"
                     /translation="MTVQPLRLAGKSGYGVLLVHGLTGAPAEMKPLAKRLARRGFEVA
                     APLLAGHGADEATLLKTSWRDWLDGLCEAYDAFAAQVETVHVAGICVGGALGLMLAAE
                     RPAIRAAAVYSMTFEYDGWNMPRFAVAAPLIQLVANLPGVRRLAWAETYPFGLKDERL
                     RSLAERAPFSLIEGAIDRLPLGALFQMYRLGRHLERMGKAITTP"
     gene            complement(609..977)
                     /locus_tag="PLH31_11895"
     CDS             complement(609..977)
                     /locus_tag="PLH31_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQR89933.1"
                     /translation="MTPTLTAVSAGLLAFCIVTEIGRELCFKAASDGAEGKPAYLAAL
                     ALQPVLWLGIILWFVEMIAWLLVLEHTPLGLAFPIMTLTYAGVPLAGTLILKERLAPM
                     QVAGAALVAVGVTCVGLTGL"
     gene            complement(1044..1859)
                     /locus_tag="PLH31_11900"
     CDS             complement(1044..1859)
                     /locus_tag="PLH31_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HQR89934.1"
                     /translation="MRPIFLNLDNALAEQPALLRQGKLQVIYACDLGPALRLWSRPRA
                     LAALADRLREQPTPAGPELVFAGSGDFHHVTPLLIERAIQAANCGPVTVVHFDNHPDW
                     VRFDNGMHCGSWVGPAARLDGVAKVITVGVCSADIDHPETNRADLDLVAERRVEIYPY
                     RAPDGGLIFKVGDQGWPSIEAMGEVRFVAFLASRITTPAVYITIDKDVLRASDAITNW
                     DQGQLSLSGLEAMIRAVAGRRRLVAAAIWTWPPGGSAEDLRGLPRPAARRTTG"
     gene            complement(1868..>2205)
                     /locus_tag="PLH31_11905"
     CDS             complement(1868..>2205)
                     /locus_tag="PLH31_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR89935.1"
                     /translation="VMEKGAYFLAGLKELQKRWPQIGDVDGLGLALRAEICEADGFTP
                     NKALLNRMESEGMKGDIAVDGKCYGLILDVGGYHKNVITLAPSLEISTDEIDLALKLL
                     DAVLRRCVD"
BASE COUNT          413 a          710 c          731 g          351 t
ORIGIN      
        1 cggcgtcgtg atcgccttgc ccattcgctc cagatgccgt cctaggcgat acatctggaa
       61 cagagcgcca agcggcaagc gatcgatcgc gccctcgatc aggctgaagg gcgcgcgttc
      121 ggccagactt cgcaggcgtt cgtccttcag accgaaagga taggtttcag cccaggccag
      181 ccggcgcacg ccaggcagat tagcgaccag ctggatcaac ggcgcggcga cggcgaagcg
      241 cggcatgttc cagccgtcat attcgaaagt catcgaatag accgcggcgg cgcggatagc
      301 cggccgctcg gcggccagca tcagacccaa ggctccaccg acgcagatcc cggccacatg
      361 caccgtctcg acctgggcgg cgaaagcgtc gtaggcctcg cacaggccgt cgagccagtc
      421 gcgccagctg gtcttgagca aggtcgcctc gtcggctccg tgtcccgcca gcaggggcgc
      481 ggcgacctcg aatcctcgac gcgccaggcg cttggccagg ggcttcatct cggccggcgc
      541 accggtgagg ccgtgaacca gcaggacacc atagccggac ttgccggcta gtctgagcgg
      601 ttgaacggtc ataggcctgt gagcccaaca caggtgacgc cgacggcgac cagcgccgcg
      661 cccgcgacct gcatcggggc caggcgctcc ttcaggatca aggtcccggc caggggcacg
      721 ccagcatagg tcagggtcat gatcgggaag gccagaccca gcggcgtgtg ctccaacacc
      781 aacagccagg cgatcatctc gacgaaccag agaatgatcc caagccacag gaccggctgc
      841 aaggccagcg ccgcgagata ggccggcttg ccctccgcgc cgtcggacgc ggccttgaag
      901 cagagctcac ggccgatctc ggtgacgatg cagaaggcca gcaggccagc cgagacggcg
      961 gtcagggtgg gggtcaagcg gtgacctccg aaaacaagga aagcagccgc aggtttgccg
     1021 cctcgttgat atggcgcgcg gcgtcagccg gtggtcctgc gtgcggctgg tcgaggaagg
     1081 cctcgcagat cttcagcaga gccgccaggg ggccaggtcc atatcgcggc ggcgaccagt
     1141 cgccgacgac cggcgacggc gcggatcatg gcttcaaggc cgctcaggct gagctggccc
     1201 tggtcccagt tagtgatagc gtcgctggcg cgcagcacgt ccttatcgat tgtgatgtag
     1261 accgccggcg tggtgatgcg tgaggccagg aacgccacga accggacctc gcccatggct
     1321 tcgattgacg gccagccctg atcaccgacc ttgaatatca atccgccgtc aggcgcacgg
     1381 tagggataga tctccactcg tcgctcggcg acgaggtcga ggtcagcgcg attggtttca
     1441 ggatggtcga tgtcggcgct gcagactccg acggtgataa ccttggccac accgtccagc
     1501 cgcgccgcgg ggccgaccca tgagccgcag tgcattccgt tgtcgaatcg cacccagtcg
     1561 ggatggttgt cgaaatggac taccgtcact ggaccgcaat tcgcggcctg gatggcgcgt
     1621 tcgatcaaca gaggcgtgac gtgatggaag tctcccgagc cggcgaacac cagttcggga
     1681 cccgccgggg tcggctgctc gcgcaggcgg tcggcgagcg ccgccaaggc gcgaggccgg
     1741 ctccacaatc ttagagccgg ccccagatca caggcgtata tcacctgtaa tttaccctgg
     1801 cgaagcagcg cgggctgctc agccaaggcg ttgtccaggt tcaggaagat gggacgcatg
     1861 cgactgatca gtcgacgcag cgacgcagaa cggcgtcgag cagctttagg gccaggtcga
     1921 tctcgtcagt gctgatctcc agggacgggg ccagggttat gacgttcttg tggtagccgc
     1981 ccacgtcgag gatcaggccg tagcatttgc cgtcgaccgc gatgtcgccc ttcatccctt
     2041 cggattccat gcggttgagc agggccttgt tgggggtgaa accatcggcc tcacagatct
     2101 cggcgcgcag ggccaggcca agaccatcta cgtcgccgat ctgcggccaa cgcttttgca
     2161 gttccttgag gccggctagg aagtaggcgc ccttctccat gaccc
//
LOCUS       DAOMEO010000539         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5228_length_2205_cov_2.345581, whole genome shotgun sequence.
ACCESSION   DAOMEO010000539 DAOMEO010000000
VERSION     DAOMEO010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_2205_cov_2.345581"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLH31_11910"
     CDS             complement(<1..>257)
                     /locus_tag="PLH31_11910"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518883.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQR89936.1"
                     /translation="AVSATQSPAMDIQVASNFERLYFEAVRRDGVETGRAFRGFNDSG
                     LIDIPPSAFAMMRELFRGAAVSEADTAKTMLSTLNETGEVI"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<268..1146)
                     /gene="thrC"
                     /locus_tag="PLH31_11915"
     CDS             complement(<268..1146)
                     /gene="thrC"
                     /locus_tag="PLH31_11915"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQR89937.1"
                     /translation="MRYVSTRGEAAPIGFLDAVLAGLAPDGGLYVPAEWPTFTKAEIA
                     AFAGQPYAQVAAAVMGKFVGDDIPADVLADMCAEAYASFAHSAVTPLKQLGPNRFLLE
                     LFHGPTLAFKDVAMQLLGRLYDYVLERQSRKMTIICATSGDTGGAAVEAFRGRKNARI
                     VALFPEGRISEVQRRFMTTATDTNVACVSILGSFDDCQAIVKQAFQDDQFRHAVDLSG
                     VNSINWARIAAQSVYYFTAAVALGAPERSVAFVVPTGNFGDAYAAFVATRMGLPIAQI
                     ICATNSNDILARTFEDG"
     gene            complement(1146..1850)
                     /locus_tag="PLH31_11920"
     CDS             complement(1146..1850)
                     /locus_tag="PLH31_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HQR89938.1"
                     /translation="MSESFPKPFDFTARRRFPIGLTLAVAVVFGILCFLGVWQLKRMV
                     WKEDLLARIETLKTAQAVPIGPVLAQAARGENVAWTRVSVACRPAALPPLSLAYGIRD
                     GDVVWRAQALCGVPEGPYDLILIDRGVAPDLTGQVEAPARAFLEPGQVTGILIPLAQL
                     GGDRAAAVTGLAGRRPAPVALMVESESPAPAGVVPAPLPAEISNRHLEYALTWFGLAV
                     TLLFIYAAMLWRRLRP"
     gene            complement(1843..>2205)
                     /locus_tag="PLH31_11925"
     CDS             complement(1843..>2205)
                     /locus_tag="PLH31_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQR89939.1"
                     /translation="MAHVNPYLAGASGRCPKCGEGFLFEGFLKIAPTCEACGFALGRH
                     ETGDGASTFIILIGGIFGAFGALFSMLAWNWPVWLLLLVWLPVTLALSLGLMRPAKGL
                     MVAAQVANKASEAGRHDV"
BASE COUNT          384 a          718 c          793 g          300 t
ORIGIN      
        1 tcgatgacct cgccggtctc gttgagggtc gagagcatgg tcttggcggt gtcggcctcg
       61 ctgaccgccg cgccgcggaa cagctcgcgc atcatcgcaa acgcgctggg cggaatgtcg
      121 atcaggccgc tgtcgttgaa gccccggaag gcgcggccgg tctcgacgcc gtcgcggcgc
      181 acggcctcga aatagaggcg ctcgaaattg gaggccacct ggatgtccat ggccgggctc
      241 tgggtggccg acaccgcnnn nnnnnnngcc gtcctcgaag gtgcgggcca ggatgtcgtt
      301 ggaattggtg gcgcagatga tctgggcgat cggcaggccc atgcgggtgg ccacgaaggc
      361 ggcataggcg tcgccgaaat tgccggtggg cacgacgaag gccaccgaac gctccggcgc
      421 gcccagggcc acggcggcgg tgaaataata gacgctctgg gccgcgatcc gcgcccagtt
      481 gatcgagttg acgcccgaca ggtcgaccgc gtgacggaac tggtcgtcct ggaaggcctg
      541 cttgacgatg gcctgacagt cgtcgaacga gcccaggatc gagacgcagg ccacgttggt
      601 gtcggtcgcc gtggtcatga accggcgctg gacctcgctg atccggcctt cggggaagag
      661 ggccacgatg cgggcgttct tgcggccccg gaaggcctcg accgccgcgc cgccggtgtc
      721 gcccgaggtg gcgcagatga tggtcatctt ccgcgactgg cgttccagca catagtcgta
      781 cagccgcccc agcagctgca tggcgacgtc cttgaacgcc agggtcgggc cgtggaacag
      841 ctccagcagg aagcggttgg gacccagctg cttgagcggc gtgacggcgc tgtgcgcgaa
      901 gctggcatag gcctcggcgc acatgtcggc cagcacgtcg gcggggatgt cgtcgcccac
      961 gaacttgccc atcacggcgg cggcgacctg ggcgtagggc tggcccgcga aggcggcgat
     1021 ctcggctttc gtaaaggtcg gccactcggc cggcacatac agaccgccgt cgggggccag
     1081 gcccgccagc acggcgtcga ggaatccgat gggcgcggcc tcgccgcggg ttgaaacgta
     1141 gcgcatcagg gtctcagtct ccgccagagc atggcggcat agataaagag cagggttacg
     1201 gcaagaccga accacgtcag cgcatattcc aggtgccgat tggaaatttc tgccggcagc
     1261 ggcgcgggga cgacgccggc cggggccggg ctctcgcttt ccaccatcag ggccacgggc
     1321 gcgggcctgc gtccggccag gccggtcact gcggcggcgc ggtccccgcc cagctgggcc
     1381 agggggatca ggatgccggt cacctgcccg ggttccagga acgcgcgggc cggggcctcc
     1441 acctggccgg tgaggtcggg cgccacgccg cgatcgatca ggatcagatc atagggcccc
     1501 tccggcacgc cgcacagggc ctgggcccgc cagacgacat cgccgtcgcg aatgccatag
     1561 gccagggaca ggggcggcag ggcggccgga cgacaggcga cactgacccg ggtccaggcg
     1621 acattctcgc cccgggccgc ctgggccaga accgggccga tcggaacggc ctgggcggtc
     1681 ttcagggtct cgatccgcgc cagaagatct tccttccaga ccatgcgctt gagttgccac
     1741 acgcccagaa agcagaggat gccaaacacg acggccaccg cgagggtcag gccgatgggg
     1801 aagcgccgcc gcgccgtgaa atcgaacggc ttgggaaagc tctcagacat cgtgacgacc
     1861 ggcctccgaa gccttgttgg cgacctgggc ggccaccatc aggcccttgg cgggtcgcat
     1921 caggcccagg ctgagcgcca gggtcaccgg cagccagacc aggagcagca gccagaccgg
     1981 ccagttccag gcgagcatcg aaaacagagc cccgaaggcg ccgaagatgc cgccgatcag
     2041 gatgatgaag gtcgaggcgc cgtcaccggt ctcgtggcgg cccagcgcaa agccgcaggc
     2101 ctcgcaggtg ggggcgatct tcaggaagcc ctcgaacaga aacccctcgc cgcacttggg
     2161 gcaacggccg gacgcgccgg cgagataggg gttcacgtga gccat
//
LOCUS       DAOMEO010000540         2204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5230_length_2204_cov_2.126105, whole genome shotgun sequence.
ACCESSION   DAOMEO010000540 DAOMEO010000000
VERSION     DAOMEO010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_2204_cov_2.126105"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLH31_11930"
     CDS             complement(<1..805)
                     /locus_tag="PLH31_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR89940.1"
                     /translation="MPKLTSAIDPSGETFARNAAHNRSLVEELRERVATAALGGPEAT
                     RQRHLARGKLLPRQRVERLLDPGSPFLEIGQLAACDLYNGEAPGAGVITGIGRVSGRE
                     VMIVANDATVKGGAYFPITVKKHLRAQEIAQQNRLPCVYLVDSGGANLPHQAEVFPDR
                     DHFGRIFFNQARMSAAGIAQIACVMGSCTAGGAYVPAMSDETVIVRDQGTIFLAGPPL
                     VKAATGEVISAEELGGADTHGRRSGVVDHVANDDEHALEIVRSIVANLNT"
     gene            973..>2204
                     /locus_tag="PLH31_11935"
     CDS             973..>2204
                     /locus_tag="PLH31_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR89941.1"
                     /translation="MGTPLDAFNDSALARLFARERRDGSAAWFSLPGGATLYAPGEAA
                     DQLYFLRTGRLGAFRREEGQEPQLLGVIRPGEPAGEMALIGGTPHSANLVALRDSEVL
                     ALPRADFFEAAESDPTVMLELSRLMIRRARQAQTHAAIGDPSVFGFIAVEAGLAIRPV
                     VERLARCIETLGYSVTVEGGESLLAPTEWFSNVEREHDFVLYVAEADETQWKYLVGRQ
                     VDHLFRIGRGDRAPPADIPTYASGPLQAQRLVDLILLQSSSLKQPQGSEAWMEATQAA
                     RLFHLRENGMADVQRLARVLTGQSVGLVLSGGGARAYAHIGAIQAMRERGIPIDFVGG
                     ASMGAVIAAGLAMGWDDGEMETRIRQAFVESSPLDDIAFPMIAMTRGDKVKARLEEHF
                     GDIQISDLWLPFFCVSSNL"
BASE COUNT          340 a          739 c          751 g          374 t
ORIGIN      
        1 tcgtgttgag attggcgacg atcgagcgga cgatctccag cgcatgctca tcatcattgg
       61 cgacgtgatc gaccacgcca gagcggcggc cgtgggtgtc ggccccgccc agttcctcgg
      121 cgctgatgac ttcgcccgta gcggccttca ccagcggcgg cccggccagg aagatggtgc
      181 cctggtcgcg gacaatgacg gtctcgtcgc tcatggcggg cacataggcg ccgccggcgg
      241 tgcaggatcc catgacgcag gcgatctggg cgatgcccgc cgcgctcatc cgcgcctgat
      301 tgaagaagat ccggccaaaa tggtcgcggt cgggaaacac ctcggcctga tggggcaggt
      361 tcgcgccgcc gctatcgacc agatagacgc acggcaagcg gttttgctga gcgatctcct
      421 gggcgcgaag gtgcttcttc acggtgatcg ggaaataggc cccgcccttc accgtggcgt
      481 cattggcgac gatcatcact tcgcggcctg atacgcggcc gatgccggtg atcacgccgg
      541 cgccgggcgc ctcgccgttg tagaggtcac aggcggccag ctggccgatc tccaggaacg
      601 gcgaacccgg atccagcagg cgctcgacgc gctggcgcgg caacagcttg ccccgcgcca
      661 aatggcgctg gcgcgtggcc tcggggccgc ccagggcggc cgtggccacc cgttcgcgca
      721 gttcttcgac gaggctgcga ttgtgggcgg cgttgcgggc gaaggtctcg ccggacggat
      781 cgatagcgga ggtcagcttc ggcatatgag cggcatagcc ttttccattc aaacccggaa
      841 ggcggtcatt ttcgcctccc gttcagtaat agcccgcaag aaccctccac gaccaagacg
      901 ggtcgagggt gcgacagtcg gaccgatcaa cgcccgatgg tcaagccatg gcattcacgc
      961 tagcgttcgg ctgtgggaac gcctttggac gccttcaacg actcagccct ggcgcgcctg
     1021 ttcgcccgtg agcggcgcga cgggtcggcg gcatggttct ccctgcccgg cggcgcaacc
     1081 ttgtatgcgc ccggcgaggc ggccgaccag ctctactttc tgcgcaccgg acggctgggc
     1141 gcgtttcggc gcgaggaagg ccaggaaccg cagttgctgg gcgtgatccg tcccggcgag
     1201 ccggctggcg aaatggccct gattggcggc accccacact cggccaacct tgtggccctg
     1261 cgcgacagcg aggtgctggc cctgccccgc gccgacttct tcgaggccgc cgagagcgat
     1321 ccgacggtga tgctggagct gtcgcgactg atgatccgcc gcgcccgcca ggcccagacc
     1381 cacgccgcca tcggcgatcc ctcggtgttc ggcttcatcg ccgtcgaagc gggcctggcg
     1441 attcggccgg tcgtcgaacg cctggcgcga tgtatcgaaa ccctgggcta ttcggtcact
     1501 gtcgaaggcg gcgagtcgct gctggcgccg accgagtggt tcagcaatgt cgagcgcgag
     1561 cacgacttcg tcctctacgt cgccgaggcc gacgagaccc agtggaaata cttggtcggc
     1621 cgccaggtgg accatctgtt ccggatcggg cgtggcgacc gtgccccgcc ggcggatatc
     1681 cccacctatg cctccgggcc cctgcaggcc cagcggctgg tcgacctgat cctgctgcaa
     1741 tcgagcagcc tgaagcagcc gcagggctcc gaggcctgga tggaggccac ccaggccgcc
     1801 cgtctgttcc atctgcgcga gaacggcatg gccgatgttc agcggctggc gcgggtgctg
     1861 accggccagt cggtcggcct ggtgctgtcg ggcggcggcg cgcgcgccta tgctcacatc
     1921 ggcgccatcc aggcgatgcg cgagcgcggc attcccatcg acttcgtcgg cggggcctcg
     1981 atgggcgccg tgatcgcggc gggcctggcc atgggctggg acgacggcga gatggagacc
     2041 cgcatccgcc aggccttcgt ggaatccagc cccctggatg acatcgcctt cccgatgatc
     2101 gccatgaccc gcggcgacaa ggtcaaggcg cgactggagg aacatttcgg cgacatccag
     2161 atcagtgact tgtggttgcc gttcttctgc gtttcctcga acct
//
LOCUS       DAOMEO010000541         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5245_length_2200_cov_2.255944, whole genome shotgun sequence.
ACCESSION   DAOMEO010000541 DAOMEO010000000
VERSION     DAOMEO010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5245_length_2200_cov_2.255944"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLH31_11940"
     CDS             <1..228
                     /locus_tag="PLH31_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQR89942.1"
                     /translation="ERHVMISNETIALFVRFWLTAVPPLPDTAQAAAQAKGRERFEGF
                     VETLGRRLAKGRSFADEILRDVPAETEADED"
     gene            416..1351
                     /gene="trbB"
                     /locus_tag="PLH31_11945"
     CDS             416..1351
                     /gene="trbB"
                     /locus_tag="PLH31_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HQR89943.1"
                     /translation="MLRTALGADIAAFLDDPAIVEVMLNPDGRLWIDRLAGGLEDTGA
                     RLSAADGERIVRLVAHHVGAEVHAGNPRVSAELPETGERFEGLLPPVVAAPSFAIRKP
                     AVAVFTLSDYVEAGIMRADQAQSLRAAVHARKNILVAGGTSTGKTTLVNALLAEVAQT
                     GDRVVLIEDTRELQCAAPNLVALRTKDGAASLTDLVRSALRLRPDRIPIGEVRGGEAL
                     DLIKAWGTGHPGGVGTLHAGSALGALRRLEQLIQEAVITVPRALIAETIDLIAVLSGR
                     GAARRLAELTHVEGLGPDGDYRLVPQLPSSPGDLP"
     gene            1348..1665
                     /locus_tag="PLH31_11950"
     CDS             1348..1665
                     /locus_tag="PLH31_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HQR89944.1"
                     /translation="MTPLCRRLTGAATFALFAIVTAPAYAAGSNMPWEAPLQSILESI
                     QGPVAKIIAVMIIIVTGLTLAFGDTSGGARKMIQIVFGLSIAFAASSFFLSFFSFGGG
                     ALI"
     gene            1665..1937
                     /locus_tag="PLH31_11955"
     CDS             1665..1937
                     /locus_tag="PLH31_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HQR89945.1"
                     /translation="MLGADEPAVGFYAPVRRSLTEPILLGGAPRSLAIINGTLAAALG
                     LGLRLWLAGLLIWFVGHMAAVWAAKRDPAFVEVVRRHLRYPTHLGA"
     gene            1948..>2200
                     /locus_tag="PLH31_11960"
     CDS             1948..>2200
                     /locus_tag="PLH31_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395128.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR89946.1"
                     /translation="MLNLAEYRGKSQSLSDFLPWAALVSEGVILNKDGSFQRTARFRG
                     PDLDSATPAELVATTSRLNNALRRLGSGWAIFAEAQGHAA"
BASE COUNT          312 a          821 c          696 g          371 t
ORIGIN      
        1 gagcgccacg tcatgatcag caatgagacg atcgccctgt tcgtgcgctt ctggctgacg
       61 gccgttccgc cactgcccga caccgcccag gccgccgcgc aagccaaggg ccgcgagcgc
      121 ttcgaaggct ttgtcgagac cttgggccgg cgactcgcca agggccgcag cttcgccgac
      181 gaaatcctcc gcgacgttcc cgccgagacc gaagccgacg aggactaaaa ccgccgtttc
      241 cctgccgttg tacgccagcg cactactacg ccctcgtcgc tgttgaaccc gcctcagatc
      301 gccgcttctt gattgacccg ttgaggatct cgccttcggc gagacgcgag gaaacgggac
      361 gacatcagtg accacggtcg cattccgatc cgaaagcttc gcccgcggcg cgcgcatgct
      421 gcgcaccgcg ctcggcgccg acatcgccgc cttcctcgac gaccccgcca tcgtcgaggt
      481 gatgctcaat cccgatggac gcctctggat cgaccggctg gccggcggcc tggaagacac
      541 cggcgcgcgc ctcagcgccg ccgatggcga gcgcatcgtg cgccttgtcg cccaccacgt
      601 cggcgccgag gtccatgccg gcaatccgcg cgtctccgcc gagctgcccg agacgggaga
      661 gcgcttcgag gggctgcttc cgcctgtggt ggcggcgccc agcttcgcga tccgcaagcc
      721 tgcggtcgcc gtcttcaccc tgtcggacta tgtcgaggcg gggatcatgc gcgcggatca
      781 ggcgcagagc ctgcgcgccg ccgtccacgc ccgtaagaac atcctggtcg ccggcggcac
      841 ctcgaccggc aagaccaccc tggtcaatgc gctgctggcc gaggtggcgc agacgggcga
      901 ccgcgttgtc ctcattgagg acacgcgcga actgcaatgc gccgcgccca atcttgtggc
      961 cctgcgcacc aaggacggtg ctgcgagcct gaccgatctg gtccgctcgg cgcttcggct
     1021 gcgccccgac cgcattccca tcggcgaagt gcgcggcggt gaggcgctcg atctgatcaa
     1081 ggcctggggc accggccatc ccggcggcgt cggcactctg cacgcagggt cagcgctcgg
     1141 ggcgctgcgt cggctcgaac aactgatcca ggaggcggtg atcaccgtcc cgcgcgcgct
     1201 gatcgctgaa accatcgatc tcattgcggt tctgtcgggc cgcggcgccg cccgccgcct
     1261 ggccgaactc acgcacgtcg aggggctcgg ccccgacggc gactaccggc tcgtccctca
     1321 gctgccttcc tcgccaggag atctgccatg acccctcttt gccgtcgcct caccggcgcc
     1381 gcgaccttcg ccttgttcgc catcgtgacc gcgccggcct atgcggccgg ctccaacatg
     1441 ccttgggaag ccccactcca gtcgatcctg gagtcgatcc aggggccggt ggccaagatc
     1501 atcgcggtga tgatcatcat cgtcaccggt ctcaccctgg cgttcggcga cacctcgggc
     1561 ggcgcccgca agatgatcca gatcgtcttt ggcctttcga tcgccttcgc cgccagctcc
     1621 ttcttcctgt ccttcttctc ctttggcggc ggggcgctga tctgatgctc ggcgcggacg
     1681 aacccgccgt cggcttctat gcgccggtcc ggcgcagcct caccgagccg atcctgctcg
     1741 gcggcgcgcc gcggtcactg gccatcatca acggcacgct tgccgccgcc ttgggcctgg
     1801 gcctccgctt gtggctggcc ggcctgctga tctggttcgt cgggcacatg gccgcagtgt
     1861 gggccgcaaa gcgcgacccc gccttcgtcg aggttgtccg ccgccacctg cgctacccca
     1921 cccatctcgg cgcatgaggc aggcgcgatg ctgaatctcg ctgaatatcg cggcaagtcg
     1981 caaagcctct ccgacttcct gccctgggcg gcgctggtga gcgagggcgt gatcctcaac
     2041 aaggatggga gctttcaacg cacggcccgg ttccgcgggc cggatctcga tagcgccacg
     2101 ccggccgagc tcgtcgccac cacctcacgg ctcaacaacg cgctgcgccg gctcggctcg
     2161 ggctgggcca tcttcgccga ggcccagggc catgcggcgg
//
LOCUS       DAOMEO010000542         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5257_length_2195_cov_3.240654, whole genome shotgun sequence.
ACCESSION   DAOMEO010000542 DAOMEO010000000
VERSION     DAOMEO010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_2195_cov_3.240654"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLH31_11965"
     CDS             complement(<1..1059)
                     /locus_tag="PLH31_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HQR89947.1"
                     /translation="MRILHTSDWHLGRTLAQESLLDDQAQLLDQVFAAIVEHRVDVLI
                     IAGDLFDRAVPRKEAVQLFNDFLARVYAETGAAIVAIAGNHDAPDRVSFNSALQDPRR
                     VLIRGPLRDRPHALVLSDEHGQVAFSALPYCEIFAAREAFDDVGIASCAHALSAQLAQ
                     ARLHVPPGARWVVTAHAFVEGGRITETERPLAQVGGIETVPSAMFDGAAYVALGHLHR
                     AQEAGAAHIRYCGSWMGFGFDEADELKTLSLVELDAAGVARVTELPLVSPRPLRVVTG
                     KLADLVAAGRAPDALGAGALIKAVLTDEGALIDAIGQLRAVYPHVLQLERLNRLAAVG
                     PGAGAAAVDRQDTVKLVAG"
     gene            1130..1939
                     /locus_tag="PLH31_11970"
     CDS             1130..1939
                     /locus_tag="PLH31_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQR89948.1"
                     /translation="MSSPTPRYAYEARQRFLDGLLFWEGRVNRRDLIDTFRVSQPQAA
                     LDLKAYLAALPSGQVIYDTRQRRYEAASTFEPLFGPPALESWLERSRQAGLAVEVLPT
                     LDRPLDVGLMARLYRAIRDRKTIHVAYQTMRRATAEDRSITPTAFVSDGQRWHVRAYC
                     HLREDFRDFVLSRIAMAPNQAQAESAAVDLPLDTDWCSWVTLTLAPAAHLEENQKRAV
                     CWDYGIDGELSVTVRRALEFYAMRRWGLDRPESRLSVVGRTESAPNPEDHS"
     gene            1984..>2195
                     /locus_tag="PLH31_11975"
     CDS             1984..>2195
                     /locus_tag="PLH31_11975"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HQR89949.1"
                     /translation="MILRRVDPTRNMSRFWSARLQPSLFDEVLLVRNWGRIGSRGQEK
                     SYWFSSRQAAIAAFNKITAAKCRRGYA"
BASE COUNT          399 a          731 c          690 g          375 t
ORIGIN      
        1 gccggccacc agcttcaccg tgtcttgccg atcaaccgcc gctgcgccgg cgcccgggcc
       61 gacagcggcg aggcggttca ggcgttccaa ctgcagcacg tggggataga ccgcgcgcaa
      121 ctgaccgatg gcatcgatga gtgcgccctc gtcggtcaac accgccttga tcagagcacc
      181 cgccccgagc gcgtcaggcg cgcgcccggc cgcgaccagg tccgccaact ttccggtcac
      241 gacccgcagc ggacgcgggc tgaccagcgg aagctcggtc actctagcca ctccggcggc
      301 gtccagctcc accaggctca gagtctttag ctcatcagcc tcatcgaatc cgaagcccat
      361 ccaggagccg cagtatctaa tgtgagcggc gccggcctct tgtgcgcgat gaaggtgacc
      421 tagagcgacg taggccgcgc catcgaacat cgctgacggg accgtctcga taccgcccac
      481 ctgagccaag ggccgctcgg tttcggtgat ccgtccccct tccacaaagg cgtgggccgt
      541 caccacccat cgcgcgcctg gcggaacatg caggcgggcc tgcgccagtt gggctgagag
      601 ggcgtgggcg caactggcga tgccgacatc gtcgaacgcc tccctcgccg cgaaaatctc
      661 gcagtacggc agcgccgaga aagccacctg cccgtgctca tcgctcaaga ccaaggcgtg
      721 gggtcggtct cgcaaagggc cccggatcaa cacgcgccgc ggatcctgca gcgccgagtt
      781 gaagctaacg cggtcggggg cgtcgtgatt gcccgcgatc gctacgatcg cagcgccggt
      841 ctcggcgtaa acgcgggcga ggaaatcgtt gaacagctga accgcttctt tgcgcggcac
      901 cgcccggtcg aagaggtcgc cggcgatgat cagcacgtcg acgcgatgtt cgacgatcgc
      961 tgcgaagacc tggtcgagga gctgggcctg gtcgtcgagg aggctctcct gcgccagcgt
     1021 ccggcccaga tgccaatctg atgtgtgcaa gatccgcatc tgaagcccaa cctcccgtct
     1081 atatagtttc tatatggact agagcttggc cgtcaactag actctcggca tgtcgtctcc
     1141 cacgccgcga tacgcgtatg aagctaggca aaggtttctc gacggacttc tcttttggga
     1201 aggccgggtc aatcgccgcg acctgatcga cacgttccgg gtgtcgcagc cgcaggcggc
     1261 gctggacctc aaggcatacc tcgccgccct gccctcagga caggtgatct acgatactcg
     1321 ccaaagacga tacgaggccg cgtcgacgtt tgaaccgctg ttcggtccgc ccgccctcga
     1381 gtcctggctg gaacggtcac ggcaggctgg actggcggtt gaagtgctgc cgactttgga
     1441 caggccactc gatgtcggcc tgatggcgcg gctgtaccgg gccatccggg atcgcaagac
     1501 aatccatgtc gcctaccaga ccatgcgacg cgccacagcg gaggatcggt cgatcacgcc
     1561 tacagcgttc gtgtcagacg gtcagcgctg gcatgttcgg gcctactgcc acctgcgcga
     1621 agacttcaga gactttgtcc tttcgcggat cgccatggcg ccgaaccaag ctcaggcgga
     1681 gtctgcagcc gtcgatctgc cgctcgatac cgactggtgc tcttgggtga ccctcaccct
     1741 ggcgccggcc gctcaccttg aggaaaacca aaagcgggcg gtgtgctggg actacgggat
     1801 cgacggcgaa ctgtcggtca ccgtccggcg ggctcttgaa ttctacgcga tgcgtcgctg
     1861 gggcctcgat cggccggaaa gccgcctaag cgtggtcggg cgcactgaat ctgctccaaa
     1921 cccggaggat cactcgtgag ctcggcaacc gcccaacagg ccctggcgac cgactggccg
     1981 cggatgatcc ttcgccgcgt cgatcccacg cgcaacatgt cgaggttctg gtcggcgcga
     2041 ctccagccaa gcctctttga cgaggtgctg ctggtgcgca actgggggcg catcggctcg
     2101 cggggccaag agaagtccta ctggttttca agcaggcaag ccgccatagc ggcgttcaat
     2161 aaaataacgg ccgctaaatg ccgccgcggt tacgc
//
LOCUS       DAOMEO010000543         2183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5291_length_2183_cov_2.608553, whole genome shotgun sequence.
ACCESSION   DAOMEO010000543 DAOMEO010000000
VERSION     DAOMEO010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5291_length_2183_cov_2.608553"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLH31_11980"
     CDS             <1..180
                     /locus_tag="PLH31_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89950.1"
                     /translation="KPRTAGAPKAFGDKASGPQTTYSPKPRGEGPAKPFKGPKPFKAK
                     GFDGGKPAGKPFKKK"
     gene            complement(226..1407)
                     /locus_tag="PLH31_11985"
     CDS             complement(226..1407)
                     /locus_tag="PLH31_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR89951.1"
                     /translation="MEQFDVVVIGAGAAGMMCAIEAGKRGRSVLVVDHAKAPGEKIRI
                     SGGGRCNFTNIHTAPAHFLSANPKFCVSALRRYRPSDFIALVKKYNIAFYEKTLGQLF
                     CDGSAKQIITLLLTEMEAARCELRLETGVDRIARVEGGFELSLSNGANIRCQSLVVAC
                     GGKSIPKMGATGFGYEIAKQFGVGVVETRPALVPLTFEVKTLERLVPLSGVALDAVVA
                     HGKTKFQEGMLFTHRGLSGPSILQISSYWREGDEIRVDMAPGVDALAALKAARTATPR
                     RAVSTVMATLLPQRVAQIVSDDEGAQGNIADCSDKVLGRLAAAVNAWTFKPVGSEGYR
                     TAEVTLGGVDTNDLDSRTMQAKDVPGLYFIGEVVDVTGWLGGFNFQWAWASGWSAGQA
                     V"
     gene            1521..>2183
                     /locus_tag="PLH31_11990"
     CDS             1521..>2183
                     /locus_tag="PLH31_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQR89952.1"
                     /translation="MTSVVPPTIGIDFGTTNTVVSMTHGDGSARLVRFPTSQGDLFAF
                     RSALSFHNLQGHGGEANERVVEAGPWAIEAYLEDPLETRFIQSFKSFAASAAFTETRI
                     LNKRYLFEDLLAAFLLKVREHAGEAMADLPPRVIVGRPVTFAGGNPDEALALQRYEAA
                     FARLGFNDIRYAYEPVGAAFFFARQLKQDATVLVADFGGGTSDFSLVRFERKADGTLA
                     STP"
BASE COUNT          371 a          763 c          691 g          358 t
ORIGIN      
        1 aagccccgca ccgccggcgc gcccaaggcg ttcggcgaca aggcttcggg tccgcagacg
       61 acctattcgc ccaagccgcg cggcgagggt ccggccaagc ctttcaaggg ccccaagccc
      121 ttcaaggcca agggcttcga cggcggcaag ccggcgggta agcccttcaa gaagaagtag
      181 ttatggaaaa ggcctctctt cggagaggcc tttttctttg ggcgtttaga ccgcttggcc
      241 agccgaccag cccgacgccc aggcccactg gaaattgaag ccgcccagcc agccggtgac
      301 gtccacgacc tcgccgatga aatagagacc gggcacgtcc ttggcctgca tggtccgcga
      361 gtccaggtcg ttggtgtcga ccccgcccag ggtgacttcg gccgtccggt agccttccga
      421 acctaccggc ttgaaggtcc aggcgttcac cgcggcggcc aagcggccca gcaccttgtc
      481 cgaacagtcg gcgatattgc cctgagcgcc ttcatcgtcg gagacgatct gggccacgcg
      541 ctgtggcagc agggtcgcca tcacggtcga gaccgcccgg cgcggcgtgg cggtgcgggc
      601 cgccttcagg gccgccaaag catcgacgcc cggggccatg tcgacccgga tctcgtcgcc
      661 ttcccgccag taggacgaga tctgcaggat cgagggaccc gacaggcccc ggtgggtgaa
      721 cagcatgcct tcctggaact tggtcttgcc gtgggcgacc acggcgtcca gcgccacgcc
      781 cgacagcggg accaggcgct ccagcgtctt gacctcgaag gtcagcggca cgagggccgg
      841 acgggtctcg acgacaccca cgccgaactg tttggcgatc tcgtagccga agccggtcgc
      901 gcccatcttg gggatcgact tgccgccaca ggccaccacc agcgactggc agcgtatatt
      961 ggcgccattg gagagcgaca gctcaaaccc gccctcgacc cgcgcgatac ggtcgacgcc
     1021 ggtttccagc cgcagttcgc agcgcgcggc ctccatctcg gtcagcagca gggtgatgat
     1081 ctgcttggcc gagccgtcgc agaacagctg gcccaacgtc ttctcgtaga aggcgatgtt
     1141 gtatttcttg accagggcga tgaagtcgct gggccggtag cggcgcaggg ccgagacgca
     1201 gaacttggga ttggccgaca agaaatgggc cggcgcggta tggatgttgg tgaagttgca
     1261 gcgcccgccg ccgctgatgc ggatcttctc gccaggcgcc ttggcgtgat cgaccaccag
     1321 caccgagcgg ccgcgtttgc cggcctcgat ggcgcacatc atgccggccg cgccggcccc
     1381 aattacaacg acgtcaaact gttccatcgc gtccttccat aggagctgcc cggcgaagtc
     1441 aatcaaacgc cgcgccagct cttgaaccca ccttcgccaa cggctacgaa ctcgccccca
     1501 tcccagagcc cggttgaccc atgacgtccg tcgtcccgcc gacgatcggc atcgattttg
     1561 gcaccaccaa caccgtcgtc tccatgacgc acggcgacgg ttcggcgcgc ctggtacggt
     1621 ttccgaccag ccagggcgac ctgttcgcct tccgctcggc gctgagcttc cacaatcttc
     1681 agggccacgg cggcgaagcc aatgagcggg tggtcgaggc cggcccctgg gcgatcgagg
     1741 cctatctgga ggatccgctg gagacgcggt tcatccagtc gttcaagagc tttgccgcca
     1801 gcgccgcgtt caccgagacc cgcatcctca acaagcgcta tctgttcgag gacctgctgg
     1861 ccgccttcct gctgaaggtg cgcgagcatg ccggcgaagc gatggctgac ctgccaccgc
     1921 gcgtcatcgt gggacggccg gtcaccttcg ccggcggcaa tcccgatgag gccctggccc
     1981 tgcagcgcta cgaggccgcc ttcgcgcgcc tgggcttcaa cgacatccgc tatgcctacg
     2041 agccggtcgg ggcggccttc ttcttcgccc gccagctgaa gcaggacgcc accgtgctgg
     2101 tcgccgactt cggcggcggc accagcgact tttccctcgt gcggttcgaa cgcaaggcgg
     2161 acggcacgct ggcctcgacc ccg
//
LOCUS       DAOMEO010000544         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5298_length_2180_cov_2.277647, whole genome shotgun sequence.
ACCESSION   DAOMEO010000544 DAOMEO010000000
VERSION     DAOMEO010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5298_length_2180_cov_2.277647"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..713)
                     /locus_tag="PLH31_11995"
     CDS             complement(363..713)
                     /locus_tag="PLH31_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89953.1"
                     /translation="MDISQLLDAANWPIALVAGLAAVASSAVTILISYARFRRSNRLA
                     DNTEAVLRGMFGRGYRTRKFETLRHFIPLEENKLREALLRAGAVRISAADDPEGEQWG
                     LLEHHRNRVFKQKA"
     gene            complement(812..1849)
                     /locus_tag="PLH31_12000"
     CDS             complement(812..1849)
                     /locus_tag="PLH31_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89954.1"
                     /translation="MASDVDEADRNTRRPISDPNPPDLIGWPTKRLIALDALAPGLLA
                     RVLSAAPMSRHAIFAALSVRLPPSADDSDFALVLRSGKAREIVSYCRSEPPEGLLGAF
                     ARMGGQPLPRATYYDLLFDIFSDPDKRLQARALRHCGYISKLTLDALTSLESKWLLPG
                     VLKRADNLMEVRDFQKFIDVLRSLPNPPTDETIERAILALGPKSKLSAVIVRLIRRAK
                     SFPDHPLVRDEVFQPLVSASDLIRASRKYENCLRHRIMGPLTGRVAYAEYKDGIAVIE
                     LRRMSEDQGWLLVGIHGPRNEPVSVEIRREIREVCSGRGIPHIVENIEDLKWRSLLRM
                     ADSGGLYDWTV"
     gene            complement(1944..>2180)
                     /locus_tag="PLH31_12005"
     CDS             complement(1944..>2180)
                     /locus_tag="PLH31_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89955.1"
                     /translation="ADLMARQALHLPHRLEVPYDFNLQSEAKEYHDLIERCRDAFGAN
                     VRWKVAVSRERAIFELANRRDAVEFALICDRPIF"
BASE COUNT          429 a          664 c          667 g          420 t
ORIGIN      
        1 cagtgcaccg gcgctaaaca aagcggaatc tgacaaatcg cgtcccctat tagccggttc
       61 tatccagtcg ctgcgaccat ggcaaagggg actggcgctc aacttttgat caccaccggc
      121 gctgacggtt cccctggact cgaagctcgt atcaagcccg ccccagcgac tggcacgcaa
      181 gccggcggcc cagcaggtgc ggcgagaccg cacccggtgc tgagggtcac cttggcgccg
      241 gaagcccaga ttccgacccg ccggctgact cacccgcccg tccccttgta tgtaggtccg
      301 gcgctccaag ttgagcggga ggggcttgag gcagcgaccg tcgggtcgca aaccgaaccg
      361 cctcaggcct tctgtttgaa cactcggttc cggtgatgct ctaagaggcc ccactgttct
      421 ccctcggggt catcagccgc gctgatccga acggcgcccg cgcgcagcaa ggcttcccgg
      481 agcttattct cttccaatgg gatgaagtgg cgcagcgtct cgaacttcct ggtccgataa
      541 cctcggccaa acataccacg gagaacggct tcggtgttgt ccgcgagccg gttgcttcgc
      601 ctgaaacgcg catagctgat cagaatcgtg acggcgctgg aggcgacggc ggccaaacct
      661 gcgaccaacg cgattggcca attggccgca tcgagcaact gagaaatgtc catggagccc
      721 ccggtcagag gctatcccat gagaccggga aggcaactgt cgatgattcc agcgcgggac
      781 ttcgccgtat gcgggttttt gctgacccgg cctatacggt ccagtcgtac agaccgccgc
      841 tgtcggccat ccggagaagg ctgcgccatt tcaagtcttc gatattttcg acgatgtggg
      901 gaatcccgcg tccagagcag acctcgcgga tttcgcgacg aatctcgacc gacaccggct
      961 cattccgggg tccgtgaatc ccgaccaaca gccaaccctg atcctcggac atgcgccgca
     1021 attcaatgac cgcgattccg tccttgtatt ccgcataggc gacccggcct gtcagcgggc
     1081 ccatgatgcg atgtctgagg cagttttcgt acttccgcga ggcacggatg aggtcgctgg
     1141 cgctgaccaa gggctggaag acttcatcgc gaaccagcgg gtgatccggg aagctttttg
     1201 ccctccggat caatctgacg atgaccgccg acagcttgga tttgggcccg agcgcgagaa
     1261 tggcccgctc gatcgtttcg tcagtcgggg gattgggcag cgatcgcagg acgtcgataa
     1321 atttctggaa atcccggact tccatgagat tgtcggcccg cttcagaacg ccgggcagaa
     1381 gccatttgga ttcgagactc gtgagcgcgt ccagcgtcag cttgctgatg tagccgcagt
     1441 gcctgagcgc acgggcctgc aaccgcttat cgggatcgct gaagatgtcg aacagaagat
     1501 cgtagtaggt cgcccgtggc aggggttgac cacccatccg ggcgaaggcg ccgagcaatc
     1561 cctccggggg ctcactgcgg cagtacgata cgatctccct ggcctttccg gatcgcagaa
     1621 cgagcgcgaa atcggagtca tccgcagacg gagggagtct cacggaaagc gcggcgaaga
     1681 tcgcgtgccg ggacatcggc gccgcgctga ggacgcgtgc caaaagaccg ggcgcgaggg
     1741 cgtcgagcgc gatcaggcgc ttggtgggcc agccaatgag gtctggcgga tttggatcag
     1801 agatcgggcg gcgggtgttg cggtcggctt cgtcaacgtc gctcgccatg tagaactctg
     1861 ctcccgtgtt tcagtacggg catggtccca caagtcgcga atctgtcgag gaaaagctcg
     1921 ttcgatctag tcctttggat ttatcagaat atcggccggt cgcagatcag agcgaactcc
     1981 acagcatctc ggcggttggc cagttcaaag attgcccgct cccgactgac tgccaccttc
     2041 catcgcacat ttgcgccgaa tgcatctctg cagcgctcaa tcagatcgtg gtactctttc
     2101 gcttccgatt ggagattgaa gtcgtacgga acctccaaac ggtgaggaag gtgcagggct
     2161 tgccgtgcca tcaaatcggc
//
LOCUS       DAOMEO010000545         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5299_length_2179_cov_2.666196, whole genome shotgun sequence.
ACCESSION   DAOMEO010000545 DAOMEO010000000
VERSION     DAOMEO010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5299_length_2179_cov_2.666196"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..558)
                     /locus_tag="PLH31_12010"
     CDS             complement(106..558)
                     /locus_tag="PLH31_12010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQR89956.1"
                     /translation="MDGGNDAPGGPRLVETGDWAGWRTWSGLDPFEDQSGPFYFREDD
                     AGLVTTAFKAETRHMNGGGFMHGGCMMTFADFSLFAIAWRALAGQHAVTVSLNGEFMG
                     PARPGDLVTATGEVTKAGGSLLFVRGLVSTGSSPMLSFSGVIKKIRGR"
     gene            680..982
                     /locus_tag="PLH31_12015"
     CDS             680..982
                     /locus_tag="PLH31_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HQR89957.1"
                     /translation="MTSPIQEAILSQLAGLPEGKSIDPMNVAKAVQPERWQQILGHVR
                     TNAIELAKAGTIVILRHNKPVDPEKFRGVYRLRTRLPGDPTSFETAAPDDDADDDI"
     gene            1193..1825
                     /locus_tag="PLH31_12020"
     CDS             1193..1825
                     /locus_tag="PLH31_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR89958.1"
                     /translation="MIIHGSSLSPFVRKTMAFATEKGLTFENKVARPGSPDPEFLEIS
                     SFRKITGFQDGDFRISDSSAIVTYLEAAYPEPNLIPLEPKARARTIWFEEFADSIFVG
                     AAGKIFFNLVVGPRFMGVESDLAAVEKATTEEMPPVLDYLEKIAPESGFLIEDRLTLA
                     DIAVASPFANLMHAGFDFSSWPKAKAWADAILARPSFATVVAKERGFLGG"
     gene            1881..>2179
                     /locus_tag="PLH31_12025"
     CDS             1881..>2179
                     /locus_tag="PLH31_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HQR89959.1"
                     /translation="MLDALLSDIAACRACAPYLPHTPRPVTRVRAETRILIAGQAPGR
                     LVHETGLPFNDPSGVRLRQWMGIDRETFYGRPEIGVAAMAFCFPGTNPKGGDYPPP"
BASE COUNT          370 a          759 c          695 g          355 t
ORIGIN      
        1 ggaggtggcc cagagggccg gagggggcta gctcggctca ggccccatta gccccctcag
       61 tcgctccgcg acagctcccc ctgaggggga gcagctaccc agtacctacc gcccccggat
      121 cttcttgatc accccggaaa agctcagcat cggcgaactg ccggtcgaga ccagcccgcg
      181 cacgaacagc agcgagccgc cggccttggt cacctcgccc gtcgcggtga ccagatcgcc
      241 gggccgcgcc gggcccatga actcgccgtt caggctgacc gtcacggcat gctggccggc
      301 cagcgcccgc caggcgatcg cgaacagcga gaagtcggcg aaggtcatca tgcagccgcc
      361 gtgcatgaag ccgccgccgt tcatgtggcg ggtctcggcc ttgaaggcgg tggtcaccag
      421 acctgcgtcg tcctcgcgga agtagaaggg gccggactgg tcctcgaacg ggtccaggcc
      481 cgaccatgtg cgccagccgg cccagtcgcc ggtctcgacc aggcgcggtc cgccgggcgc
      541 gtcattgccg ccatccatgt gcttgtcccc tcgttgttat cgccgatcag ctaggacagg
      601 gccgaaaggc gcgcaagccg gactctcgcc tctgctgaca atccgtggca tgggagcgcc
      661 catgacgacc ccgaacccca tgacgagccc gatccaagag gccatcctgt cccagctggc
      721 cggcctgccg gagggaaagt ccatcgaccc catgaacgtc gccaaggcgg tgcagcccga
      781 gcgctggcag cagatcctgg gtcacgtgcg caccaacgcc atcgagctgg ccaaggccgg
      841 gaccatcgtg atcctgcgcc acaacaagcc ggtcgatccc gagaaattcc gcggcgtcta
      901 ccgcctgcgc acccgcctgc cgggcgatcc gaccagcttt gagacggcgg ctccggatga
      961 cgacgcggac gacgatatct aacctcatat tcgctctccc cggcgaaggc cggggcccag
     1021 atcgaaccca gagaggtcga tgatctggtc agccttattt cggatcaacc ccagacgctc
     1081 aggctgagtc tggaccccgg ccttcgctgg ggagagcggg tctgggattg taagtcttga
     1141 aacgagactt aatcggtgaa cttatgcgct ccaacatcag gggagttcac caatgatcat
     1201 ccacggctcg tcgctgtcgc cgttcgtgcg caagaccatg gccttcgcca ccgagaaggg
     1261 tctgaccttc gaaaacaagg tcgcccggcc gggctcgccc gatccggagt ttctggagat
     1321 cagctcgttc aggaagatta cgggcttcca ggacggtgat ttcaggatct ccgactccag
     1381 cgccatcgtc acctatctgg aggcggccta tcccgagccc aacctgatcc cgcttgagcc
     1441 caaggcccgc gcccggacga tctggttcga ggagttcgcc gacagcatct tcgtcggtgc
     1501 ggccggcaag atcttcttca acctggtcgt cggcccgcgt ttcatgggcg tcgagagcga
     1561 cctggcggcg gtcgaaaagg ccacgacgga agaaatgccg ccggtgctgg attatctgga
     1621 gaagatcgcg cccgagagcg gctttttgat cgaagatcgc ctgaccctgg ccgacatcgc
     1681 cgtggccagc ccctttgcca acctgatgca cgccggcttt gacttctcgt cctggcccaa
     1741 ggccaaggcc tgggccgacg ccatcctggc ccggccgtcg ttcgcgacgg tggtcgccaa
     1801 ggagcggggc ttcctgggcg gttagaggtc tctggcgcgg cgacgcttgg atcagcgccg
     1861 ccgcgcccta gattgtcgcg atgctcgacg cgctcctctc cgacatcgcc gcctgccggg
     1921 cctgcgcgcc gtacctgccg cacacgcccc ggccggtgac gcgggtgcgc gcggagaccc
     1981 gcatcctgat cgccggccag gcgccgggac gcctcgtcca cgagacaggc ctgccgttca
     2041 acgatccgtc gggggtgcgg ctgcgccagt ggatggggat cgaccgcgag accttctatg
     2101 gccggcccga gatcggcgtc gcggccatgg ccttctgttt tccggggacc aaccccaagg
     2161 gcggcgacta tccgccgcc
//
LOCUS       DAOMEO010000546         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5306_length_2176_cov_2.537954, whole genome shotgun sequence.
ACCESSION   DAOMEO010000546 DAOMEO010000000
VERSION     DAOMEO010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_2176_cov_2.537954"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..300
                     /locus_tag="PLH31_12030"
     CDS             124..300
                     /locus_tag="PLH31_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HQR89960.1"
                     /translation="MLKWAIILAVVALIAGALGFTGIAGAAAGVAKILFVVFLIGFVI
                     MLVLGATIFKTVAK"
     gene            complement(346..1509)
                     /locus_tag="PLH31_12035"
     CDS             complement(346..1509)
                     /locus_tag="PLH31_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HQR89961.1"
                     /translation="MSKTMLAAFGLTAGLLLGSAACAQAPAPATPNDYAKTENWLCLP
                     GRADACATDQTTTIVAADGKTSVETFTPAANAPVDCFYVYPTVSTDPGNNSDMTIDAA
                     ERSVVEQQLNRFASQCRIYAPMYRQITLAALRKVMMGGQAGDAALAYADVRDAWNHYL
                     AHENKGRGVILIGHSQGSRMLLQLLKEEIDGKPVQTQMISALVLGMNTPVDAEGRYGT
                     IPSCASASQTGCLVSYVSFRASSPPPASSRFGRTDAAGQRAACVNPAALLAGKAQTEA
                     APLQAYLGTKPMGSSSATPKPWATDVTVTTPFASTPGLLSGRCVSEGDFTYLSVDVNA
                     DPADPRVDDITGDILIMGRPLKDWGLHLIDVNLAMGDLVALAGEQSKAYAARP"
     gene            1655..>2176
                     /locus_tag="PLH31_12040"
     CDS             1655..>2176
                     /locus_tag="PLH31_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane complex domain-containing
                     protein"
                     /protein_id="HQR89962.1"
                     /translation="MTPFRTLLLVTALAAASLATPALADGYPAELRKTPAPRPAPVKR
                     AKPATKVVYVDRVVEKPVYIDRPVERIVERRVEVPVVKVVEKIVEKPVDRFVDRPVDR
                     IVEKKVYVDRPVDRVVEKIVKVPVERIVEKPVYVDRVVERPVYVERGCDCGPRHDRRS
                     EDDRGASGAISASA"
BASE COUNT          333 a          705 c          757 g          381 t
ORIGIN      
        1 ttcgggcttg gaggccatgc ttacgggtta ccggccccgg gcggcgaaaa caaggaacgc
       61 cgtgcgctct ggagtgttgt tttgccacaa gcaccaccga cttccagaac aaggaaacgc
      121 accatgctga agtgggccat cattctcgcc gttgtcgccc tgatcgccgg ggccctgggc
      181 tttaccggca tcgccggcgc cgccgccggc gtcgccaaga tcctgtttgt cgttttcctg
      241 atcggcttcg tcatcatgct ggtcctgggc gcgacgatct tcaagaccgt cgcgaaatag
      301 ttcaggcagg cgaccggccc cagggtccgg tcgcctgtcc tgtttctacg gcctggccgc
      361 gtaggccttg gactgctcgc ccgccagggc caccaggtcg cccatcgcca ggttcacgtc
      421 gatcagatgc aggccccagt ccttcagcgg ccggcccatg atcaggatgt cgccggtgat
      481 gtcgtcgacg cgcggatcgg ccgggtctgc attcacgtcc accgacagat aggtaaagtc
      541 accctcgctg acgcagcggc cggacaacag gcccggcgtc gaggcgaacg gcgtggtcac
      601 cgtcacatcc gtggcccagg gtttgggcgt cgcggatgaa gaccccatgg gcttggtccc
      661 cagataggcc tgcaacggcg ccgcctcggt ctgggccttg cccgccagca gggcggcggg
      721 attgacgcag gcggcgcgtt ggccggcggc gtcggttcgg ccaaagcggc tgctggccgg
      781 gggcggcgag gaagcccgga acgagacata ggaaaccagg cagccggtct ggctggcgct
      841 ggcgcagctg gggatcgtgc catagcggcc ttcggcgtcg accggcgtgt tcatgcccag
      901 gaccagggcc gagatcatct gcgtctggac cggcttgccg tcgatctctt ccttcagcag
      961 ctgcagcagc atccgcgacc cctggctgtg gccgatcagg atgacgccgc gtcccttgtt
     1021 ctcgtgcgcc agatagtggt tccaggcgtc gcgcacgtca gcataggcca gggcggcgtc
     1081 gccggcctgg ccgcccatca tcaccttgcg cagggccgcc aacgtgatct ggcgatacat
     1141 cggcgcatag atccggcact ggctggcgaa gcggttcagc tgctgctcga ccaccgaacg
     1201 ctcggcggcg tcgatggtca tgtcgctatt gttccccgga tcggtggaga cggtcggata
     1261 gacatagaag cagtcgaccg gcgcgttggc ggcgggggtg aaggtctcca cgctggtctt
     1321 gccgtcagcg gcgacgatgg tggtggtctg gtcggtcgcg caggcgtccg cgcggccagg
     1381 caggcatagc cagttctcgg tcttggcgta gtcgttcggc gtggcggggg cgggcgcctg
     1441 ggcgcaggcg gcgctgccca gcagcaggcc ggcggtcagg ccaaaggcgg ccagcatggt
     1501 cttggacatc gtttccccac ttcgcgctca aacgcttgtt tgggtcagca tggcgaaaag
     1561 ctttggccgc gccaaggctt tctggcccgc ttgttgcggc gcggcgggac gccgtcccga
     1621 aacggcacgt gtttttgagt gcgagacccc gataatgacg ccgttcagga cccttctgct
     1681 cgtcaccgcc ctggcggccg cgagcctggc gacgccggcc ctggccgacg gctatcccgc
     1741 cgagctgcgc aagacgcccg cgccgcgacc cgcgccggtc aagcgcgcca agccggcgac
     1801 caaggtggtc tatgtcgatc gcgtggtcga aaagccggtc tatatcgacc gtccggtgga
     1861 gcgcatcgtc gagcgccggg tcgaggttcc cgtggtcaag gtggtcgaga agatcgtcga
     1921 gaagccggtc gaccgcttcg tggatcgtcc cgtggaccgg atcgtcgaga agaaggtcta
     1981 tgtcgaccgg cccgtcgacc gagtggtcga gaagatcgtc aaggttccgg tcgagcgcat
     2041 cgttgaaaag cccgtctatg tcgatcgggt ggtcgaacgc ccggtctatg tggagcgcgg
     2101 ctgcgactgc ggcccccgcc acgaccgccg ttccgaggac gatcgcggcg ccagcggcgc
     2161 gatcagcgcc agcgcc
//
LOCUS       DAOMEO010000547         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5314_length_2174_cov_1.996225, whole genome shotgun sequence.
ACCESSION   DAOMEO010000547 DAOMEO010000000
VERSION     DAOMEO010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5314_length_2174_cov_1.996225"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLH31_12045"
     CDS             <1..278
                     /locus_tag="PLH31_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQR89963.1"
                     /translation="HKARHNKRRLIQPAVVTELARVLKSGGRLRFATDWADYAEWTIE
                     RVLADPAFRFESETADRNAPPADHVTTRYEEKKLGDCAPVFLDFVRV"
     gene            341..727
                     /locus_tag="PLH31_12050"
     CDS             341..727
                     /locus_tag="PLH31_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89964.1"
                     /translation="MNWIVEHYQSILVISGLLTLTMLQFVFAPTRSQRSTYGEALEGP
                     LADVVVRGWGLLIGLTGGMLVWAAFHPETRQLAVGVAVISKVFYMGSLVAKGGRFLKG
                     FSGVTVLVDAVMIVLLVAGLMAGHAG"
     gene            complement(734..1267)
                     /gene="ppa"
                     /locus_tag="PLH31_12055"
     CDS             complement(734..1267)
                     /gene="ppa"
                     /locus_tag="PLH31_12055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQR89965.1"
                     /translation="MDLSKIATGVNPPYDLNAVIEIPQGGEPVKYEIDKASGALMVDR
                     FLHTAMFYPANYGFIPHTLADDGDPADIMVVGPTPVVPGAIIRCRPIGTLMMVDEAGS
                     DEKILAVPVDKLHPFYTGVNSWRDLPVILTEQIAHFFQHYKDLEKGKSTKITGWADLE
                     ETADIIRAAIKRHDDNY"
     gene            1555..>2174
                     /gene="rimP"
                     /locus_tag="PLH31_12060"
     CDS             1555..>2174
                     /gene="rimP"
                     /locus_tag="PLH31_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQR89966.1"
                     /translation="MRGKTQEDRDLIEILDPVAETVGFEIVRLRLMGGAETRRLQIMA
                     ERPMQPDGSGGDMNVEDCAKLSRAVSEILDAADPISGEYTLEVSSPGVDRPLTRLKDF
                     DDYAGLEVRIELDRVAEGRKRFKGELAGVEDGQVGLNIEGEDDTTVYFPFEWLVDAKL
                     VLTDQLMKRGAEQRAARVAADGGDADEDDVAADGGDADEDDVAADGG"
BASE COUNT          349 a          664 c          757 g          404 t
ORIGIN      
        1 ggcacaaggc ccggcacaac aagcgccgcc tgatccagcc ggcggtggtg acagagctcg
       61 cccgtgtgct gaaaagcggc ggacgcctgc gcttcgccac cgactgggcc gactatgccg
      121 aatggacgat cgagcgggtg ctggccgatc cggcgttccg cttcgaaagc gaaacggcgg
      181 atcgcaacgc cccgccggcc gatcacgtga ccacgcgcta cgaggaaaag aagctgggcg
      241 actgcgcgcc ggtgttcctg gatttcgtgc gggtctgatc ggccgcgcgt ctgctaagct
      301 tgcccccggc ccgcgtcatg acgggcgggg ggaagcgacc atgaactgga ttgtcgagca
      361 ctatcaaagc attctggtga tcagcgggct gctgaccctg accatgctgc aatttgtctt
      421 cgccccgacg cgctcgcagc gctcgaccta tggcgaggcc ctggaaggac cgcttgccga
      481 tgtggtggtg cggggctggg gtctgttgat cggcctgacc ggcggcatgc tggtctgggc
      541 ggcgttccat cccgagaccc ggcagctggc cgtcggcgtc gcggtgatca gcaaggtctt
      601 ctacatgggg tcgctggtcg ccaagggcgg ccggttcctc aagggcttct ccggcgtcac
      661 cgtgctggtc gacgccgtga tgatcgtcct gcttgtggcc ggcctgatgg ccggccacgc
      721 aggctagttc aaactagtaa ttatcgtcgt ggcgcttgat cgccgcgcgg atgatgtcgg
      781 cggtttcttc caggtcggcc cagccggtga tcttggtcga cttgcctttt tccagatcct
      841 tgtagtgctg gaagaagtgg gcgatctgct cggtgaggat caccggcagg tcgcgccagc
      901 tgttcacgcc cgtatagaag gggtgcagct tgtcgaccgg cacggccaga atcttttcgt
      961 ccgaacccgc ctcatccacc atcatcaggg tgccgatcgg ccggcagcgg atgatcgcgc
     1021 cgggaaccac cggggtcggg cccaccacca tgatgtcggc ggggtcgccg tcgtcggcca
     1081 gggtgtgggg gatgaagccg taattggccg gatagaacat cgccgtgtgc aggaagcggt
     1141 cgaccatcag ggcgccgctg gccttgtcga tctcgtactt caccggctcg ccgccctgcg
     1201 ggatctcgat gacggcgttg aggtcgtagg gcgggttgac gcccgtggcg atcttggaga
     1261 ggtccattat gactcttctg tggaatgagc accgcgggga ggcgggcctg cgcgggcgct
     1321 tgtataggct acaagtgttg cggtgcgaaa gaggccgcgc atgacggccg gtcccggagg
     1381 ctgttcccgc ggctttttga ggggcttcca accgctcgcc tatcgcgcta tatagctcct
     1441 acatcgtccg tcagcgctga gtgcgctttc gagcggcggc ccgaaaggct gccgcttttt
     1501 ctttggctgt ttggcggcga cgccctctgg ccctgttctg agttgagaaa tccgttgcgc
     1561 ggcaagacgc aggaagaccg tgatctgatc gaaattctcg atcccgtggc tgaaaccgtc
     1621 ggcttcgaga tcgtgcgcct gcgcctgatg ggcggggccg agacgcgccg tctgcagatc
     1681 atggccgagc gtccgatgca gcccgatggt tcgggcggcg acatgaatgt cgaagattgc
     1741 gccaagctgt cgcgcgccgt gtccgaaatc ctggacgccg ccgatccgat cagcggcgag
     1801 tacaccctgg aagtctccag ccctggcgtc gatcgcccgc tgacgcgact gaaggatttc
     1861 gacgactatg ccggccttga ggtgcgcatc gagctggatc gcgtcgccga gggccgcaag
     1921 cgcttcaagg gcgagctggc cggcgtcgag gacggccagg tcggcctcaa catcgagggc
     1981 gaggacgaca ccaccgtcta tttcccgttc gagtggctgg tcgacgccaa gctggtgctg
     2041 accgatcagt tgatgaagcg cggcgctgaa cagcgcgccg cccgcgtcgc cgccgacggc
     2101 ggagacgcag acgaggatga cgtcgccgcc gacggcggag acgcagacga ggatgacgtc
     2161 gccgccgacg gcgg
//
LOCUS       DAOMEO010000548         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5336_length_2167_cov_2.827652, whole genome shotgun sequence.
ACCESSION   DAOMEO010000548 DAOMEO010000000
VERSION     DAOMEO010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5336_length_2167_cov_2.827652"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1464)
                     /locus_tag="PLH31_12065"
     CDS             complement(28..1464)
                     /locus_tag="PLH31_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQR89967.1"
                     /translation="MRDPIDFQTVFDRIPSPYMMLDRELCFVAANRAYLAAVSRDWEA
                     LKGVNLFEAFPSEGESRQILESSLVRARDSGQVDLLPLIHYAIKRPQHLGGGFEARIW
                     SATHTPIAGLNGETHYILQHTQDITEIQRLKDVAFGGRVPPAATMLGDDVLRRAEAVQ
                     AQSQQLRQLFMQAPGFMAVLRGPDHVFELVNNTYLQLIGHRDIVGLPLGQALPEVVEQ
                     GFLTLLNSVRKTGEAFVGQQIKIALQRTPGGALEERYLDFIYQPIIEADGEVSGIFVE
                     GSDVTDRVLAQDRQRLLMDELNHRVKNTLATVQAIAQQTLRGSATPEQFAEAFESRLL
                     ALSKTHNALTDSRWEGAALGQILQQELNPYGAERVLLSGADVQLSARVALSLGMVFHE
                     LATNAAKYGALSAGGRLKVSWTMEAESLSLEWRETGGPPPSPPTRRGFGSRLIERSIR
                     GELQGSVVIDYDPEGLVCSFTVPGARDA"
     gene            1644..2051
                     /gene="sdhC"
                     /locus_tag="PLH31_12070"
     CDS             1644..2051
                     /gene="sdhC"
                     /locus_tag="PLH31_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HQR89968.1"
                     /translation="MTDTSRGLPERPMSPHLQVWRWHITMACSILHRACVIGLYAGAV
                     LLALWALALASGHESYALFTGVMGSLLGKLVLLGLTFALFFNIAYAIRQTFWDAGVGF
                     APKTADLTGAAAIAFAVVATAVTWVIAAATGAL"
     gene            2054..>2167
                     /locus_tag="PLH31_12075"
     CDS             2054..>2167
                     /locus_tag="PLH31_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89969.1"
                     /translation="MAELNSERFRTPLSRARGTGAARHGVSHFMTERVSGIA"
BASE COUNT          337 a          711 c          722 g          397 t
ORIGIN      
        1 gcagcacttg gccctaagcg ttctgcgcta ggcgtcgcgc gcgccgggca cagtgaagct
       61 acagaccagg ccttctggat cgtagtcgat caccacggag ccctgaagtt cgcctctgat
      121 gctgcgctcg atcaggcgag agccgaagcc gcgccgggtc ggtggggaag ggggcgggcc
      181 gccggtctcg cgccattcca gcgacaggct ctcggcctcc atggtccagc tgaccttcag
      241 gcgcccgccc gcagacagcg cgccatactt ggccgcgttg gtggccagtt cgtggaacac
      301 catgcccagg gacagggcca cgcgcgccga gagctgcacg tcagcgccac tgagcagcac
      361 gcgctcggcg ccgtaggggt tgagctcctg ctggaggatc tgccccagcg ccgcgccctc
      421 ccaccggctg tcggttagcg cgttgtgggt cttcgacagg gccagcaggc gcgactcgaa
      481 agcctcggcg aactgctccg gtgtggcgga cccgcgcagg gtctgctggg caatggcctg
      541 gaccgtggcc agggtgttct tcacgcggtg gttcaactcg tccatcagca ggcgctgacg
      601 gtcctgggcg agcaccctat cggtgacgtc ggagccctcg acgaagatgc cggagacctc
      661 tccgtccgcc tcgatgatcg gctggtagat gaagtcgaga taacgctctt ccagagcgcc
      721 gccgggcgtg cgttgcaacg cgatcttgat ctgctggccc acgaaggcct caccggtctt
      781 gcgcacggag ttcagcaagg tcaggaaccc ctgctcgacc acttcgggca gggcctggcc
      841 cagcggcagg ccgacgatgt cgcgatgacc gatcagctgc agataggtgt tgttgaccag
      901 ttcgaagaca tggtcgggac cgcgcagcac agccatgaaa ccgggggcct gcatgaacag
      961 ctggcgcagt tgctggctct gggcctgaac cgcttccgcc cgacgcagga cgtcgtcgcc
     1021 cagcatggtc gcggctggcg ggacccggcc gccgaaggcg acgtccttga gccgctggat
     1081 ttccgtgatg tcctgggtgt gctgcaggat gtagtgggtt tcgccattca ggccggcgat
     1141 gggcgtatgg gtcgcgctcc agatccgcgc ctcgaagccg ccgcccaggt gttggggtcg
     1201 cttgatggcg tagtggatca gtggcagcag atcgacctgg ccgctgtcgc gggcgcggac
     1261 cagggagctt tcgagaattt gccggctttc cccttccgag gggaaggcct cgaagaggtt
     1321 gacccccttc agcgcctccc agtcgcggct cactgccgcc agataggccc ggttggcggc
     1381 gacaaagcac agctcgcggt cgagcatcat gtaggggctc gggattcgat cgaacaccgt
     1441 ctggaaatcg ataggatccc gcatactgtg cgcctccccg cgcgttaccg gcagcacaat
     1501 gccagcttga ccttaaaggt tccgccgcga tcctcgatat cgcggtgaga acgtttctca
     1561 acagtggcgg ttgcgttgac acccccgtat caaggcttac atgcatctcg caacgcacaa
     1621 tggctactgg ggttggaccg atcatgaccg acacgagccg ggggctgccc gagcgcccga
     1681 tgtcaccgca tcttcaagtg tggcggtggc atatcaccat ggcttgctcg atcctgcacc
     1741 gcgcctgtgt gatcggcctc tatgccggcg cagtcctgct ggcgctttgg gccctggccc
     1801 tggcttcggg ccatgagagc tacgccctct ttacgggcgt catgggttcg ctgctgggca
     1861 agctggtgct gctgggcctg acctttgccc tgttcttcaa cattgcctat gcgatccggc
     1921 agaccttctg ggacgccggc gtgggcttcg cgcccaagac cgccgacctg acgggggccg
     1981 ccgcgatcgc cttcgccgtt gtcgctacgg ccgtcacctg ggtgatcgcc gccgccacgg
     2041 gagcgctttg atcatggccg agctgaactc cgagcgtttc cgcaccccgc tgtcgcgagc
     2101 ccgcggcacg ggcgccgccc gtcacggcgt gagccacttc atgaccgagc gtgtctcggg
     2161 catcgcg
//
LOCUS       DAOMEO010000549         2160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5361_length_2160_cov_2.891686, whole genome shotgun sequence.
ACCESSION   DAOMEO010000549 DAOMEO010000000
VERSION     DAOMEO010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_2160_cov_2.891686"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLH31_12080"
     CDS             complement(<1..633)
                     /locus_tag="PLH31_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQR89970.1"
                     /translation="MSHPGILYPKMTVIGCGLIGGSVIRAARAHGVVSQITVADASEA
                     HRARVVELGIADHVTGDIAEAVKDADLVVIATPVLSTAEVGALLAPAMKPGATLTDVG
                     STKTSVAEALRALPLDHVFVIPGHPIAGTEQSGPDAGFAELFENRWTILTPSEIDDEA
                     YAAAVAKLSSFWRSFGAQVELMDEKHHDLVMAVVSHLPHLIAYTIVGSAAD"
     gene            complement(633..1754)
                     /gene="hisC"
                     /locus_tag="PLH31_12085"
     CDS             complement(633..1754)
                     /gene="hisC"
                     /locus_tag="PLH31_12085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQR89971.1"
                     /translation="MTAPAPDRFAAPRPMPKPGILDIHAYVGGKSRIEGVAHPVKLSS
                     NENILGCSDAAKDAYRAAVDRLHLYPDGKANGLRAAVAQHYGLEPERLTFGDGSDEIF
                     ALLCQVYLEPGDNIVQGEHGFAAYAIGARACQGQVRMAKEPNQRIDADEVVKCIDERT
                     RLVFIANPANPTGTWLTGDEIRALHAAIPPSVVLVLDGAYAEFATDPRFEDGLDLARA
                     AENVIVTRTFSKIHGLGGLRVGWGYGPAGIIEPIERIRPPFNTSIPGQEAAIAALADV
                     AFQARSVALVEQWRPFLTQQLGGLGLEVTPSAANFVLVNFPRAPGKTAPEAEAFLASR
                     GYLVRAVGNYGLPDAIRITIGLEEHNRAVVALLAEFMGR"
     gene            complement(1815..>2160)
                     /locus_tag="PLH31_12090"
     CDS             complement(1815..>2160)
                     /locus_tag="PLH31_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR89972.1"
                     /translation="QLRIAPSSVTKHVQLIERAVKAALVHRTTRRVNVTEAGERFYEH
                     YRTILSQIDSAAAIVEALSPSGVAAEPLAESGGLKLFSLSGFFMPHDERLAQAPGKLT
                     GVIGAAPTPFDV"
BASE COUNT          343 a          686 c          784 g          347 t
ORIGIN      
        1 atcggccgcg ctgcccacga tcgtgtaggc gatcaggtgg ggcaggtggc tgaccacagc
       61 catgaccagg tcatggtgct tttcgtccat cagctcgacc tgggcgccga acgaccgcca
      121 gaagctgctg agcttggcga cggcggcggc ataggcctcg tcgtcgatct cggacggggt
      181 caggatcgtc cagcgattct cgaacagctc ggcgaagccg gcgtcggggc ccgactgctc
      241 ggtgccggcg atcggatggc cggggatgac gaagacgtgg tccagcggca gggcgcgcag
      301 ggcctcggcg acgctggtct tggtggagcc gacatcggtc agggtggcgc ccggcttcat
      361 ggccggcgcc agaagcgcgc ccacctcggc ggtggacagg accggggtgg cgatgaccac
      421 caggtcggcg tccttgacgg cctctgcgat gtcgcccgtg acatggtctg cgatgcccag
      481 ttccaccacg cgggcccggt gggcttcgct ggcgtcggcc acggtgatct ggctgaccac
      541 gccgtgggcg cgggctgcgc ggatcaccga accgccgatc aggccgcagc cgatcacggt
      601 catcttcgga tagaggatcc cggggtgtga catcagcggc ccatgaactc ggccagcagc
      661 gccaccacgg cccggttgtg ctcttccagg ccgatggtga tgcggatggc gtcgggcagg
      721 ccgtagttgc cgacagcgcg gaccagatag ccccggctgg ccaggaacgc ctcggcttca
      781 ggggctgtct tgcccggcgc acgggggaaa ttgaccagca cgaaattggc ggccgagggc
      841 gtgacctcaa ggccaagccc gcccagctgc tgggtcagga acggccgcca ctgttcgacc
      901 agcgccaccg agcgggcctg gaacgcgaca tcggccaggg cggcgatggc cgcctcctgg
      961 ccggggatcg aggtgttgaa cggcgggcga atgcgctcga tcggctcgat gatcccggcc
     1021 ggcccatagc cccagccgac ccgcaggccg cccaggccgt ggatcttcga aaaggtgcgc
     1081 gtgacgatga cgttttcggc tgcacgggcc aggtcgaggc catcctcaaa gcgcggatcg
     1141 gtggcgaact cggcataggc cccgtccaac accagcacca ccgaaggcgg gatggcggcg
     1201 tgcagggcgc ggatttcgtc ccccgtcagc caggtgccgg tggggttggc cggattggcg
     1261 atgaacacca gccgcgtgcg ctcatcgatg catttgacca cttcgtcggc atcgatgcgc
     1321 tggttcggct ccttggccat ccgcacctgg ccctgacagg cgcgggcccc gatcgcatag
     1381 gcggcaaagc catgctcgcc ctgaacgata ttgtcgccgg gctccagata gacttggcac
     1441 agcagggcga agatctcgtc gctgccgtcg ccgaaggtca ggcgttcggg ctccaggcca
     1501 tagtgctggg ccacggcggc gcgcaggcca ttggccttgc cgtccggata gagatgcagg
     1561 cgatcgacgg cggcgcgata ggcgtccttg gccgcatcgc tgcagcccag gatgttctcg
     1621 ttgctcgaca gctttaccgg gtgggcgacg ccctcgatcc tcgacttgcc gccgacatag
     1681 gcgtggatat ccaggatgcc cggcttgggc atggggcgcg gcgcggcgaa gcggtcgggg
     1741 gcgggagcgg tcatctcggc tactcgttga aactcggggc ggtcttacac gtcggtcgct
     1801 taaacgtcgc ccggctaaac gtcgaaaggc gtgggcgcag ccccgatgac gccagtcagc
     1861 ttgccggggg cctgagccag gcgctcgtcg tgcggcatga agaaccccga cagcgagaac
     1921 agcttcagcc cgccggattc ggccagcggc tcggcggcga cgccgctggg gctcagggcc
     1981 tcgacgatgg cggcggcgct gtcgatctgg gacaggatcg tccggtaatg ctcataaaat
     2041 ctctcgccgg cttcggtgac attgacccgt ctggtggtgc ggtggacgag agcggctttg
     2101 accgcgcgct cgatgagctg gacgtgtttg gtgaccgaag agggggcgat ccgaagttgc
//
LOCUS       DAOMEO010000550         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5364_length_2159_cov_3.120247, whole genome shotgun sequence.
ACCESSION   DAOMEO010000550 DAOMEO010000000
VERSION     DAOMEO010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5364_length_2159_cov_3.120247"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLH31_12095"
     CDS             complement(<1..720)
                     /locus_tag="PLH31_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR89973.1"
                     /translation="MGKRRMLGRDFEGFTVVVTGASTGLGRAIAVETARRGAGLIVVN
                     FASKAEDAEETARLIQAEGARAVLAQGDVSQDADCRRIVEAAAATGRIDALFNNAGMT
                     KFAPNHADLDAVDAEDFLRLYSVNVVGAFQMVRAARSLLEAAPQPGAVVNTSSIAGVV
                     GNGSSVPYAASKGAMTTMTLSLARALAPHIRVNAICPGFIDTPWFGKAMDADRVDRLR
                     AGAAAATPLKVASTAEDVAGAA"
     gene            797..1252
                     /gene="queF"
                     /locus_tag="PLH31_12100"
     CDS             797..1252
                     /gene="queF"
                     /locus_tag="PLH31_12100"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HQR89974.1"
                     /translation="MTDLNVTQLGRMVDAPASPELAVLERVPNPQADVLYLSRFVAPE
                     FTSLCPVTGQPDFAHLVIDYAPRDWLIESKSLKLYLASFRNHGSFHEDCTVKVARKIV
                     EIADPHWLRIGGYWYPRGGIPIDVFWQTGVAPEGLWVPDQGVAPYRGRG"
     gene            complement(1249..1539)
                     /locus_tag="PLH31_12105"
     CDS             complement(1249..1539)
                     /locus_tag="PLH31_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89975.1"
                     /translation="MARRKDPWAGLAWNAWAMGLEASTVIGLRTMKIAAGGAAAQAEI
                     DLMVGEKMTAAMTLPMLAMTGQLGTNGPAVAAGSISHLRKKVRANRRRLSKA"
     gene            complement(1593..>2159)
                     /locus_tag="PLH31_12110"
     CDS             complement(1593..>2159)
                     /locus_tag="PLH31_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQR89976.1"
                     /translation="ETAPEVLSFLARRRSASAMTLTAPGPDDGQLADLLRLAARVPDH
                     GKLSPWRFIILRGPAKDVFADKITALASDQANPVKANAALRKLTRPPVCVAVISRYIP
                     GEIPEWEQRQSASAVCMQMLLAASAMGWGANWITDWYAYDPRALAILGVGAGEQVAGF
                     LYLGTCSEQPQERVRPDVAAITSEWAPG"
BASE COUNT          333 a          759 c          709 g          358 t
ORIGIN      
        1 ggcggcgccg gcgacatcct cggcggtcga ggccaccttc aggggtgttg cggccgccgc
       61 tccggcccgc aggcggtcta cccggtcggc gtccatcgcc ttgccgaacc aaggggtatc
      121 gatgaagccc gggcagatgg cgttgacgcg gatgtggggc gccagcgccc gggccagcga
      181 cagggtcatg gtcgtcatcg cccccttcga ggccgcatag ggcaccgatg agccattgcc
      241 gaccacgccg gcgatggacg aggtgttgac cacggccccg ggctggggcg cggcttccag
      301 caacgaccgg gcggcgcgca ccatctggaa ggccccgacc acattaacgc tgtagagccg
      361 caggaagtcc tcggcgtcga ccgcgtccag atcggcgtgg ttgggcgcga acttggtcat
      421 accggcattg ttgaacagcg catcgatccg accggtggcg gcggcggcct cgacgatcct
      481 gcggcagtcg gcgtcctgcg agacatcacc ctgggccagc accgccctgg cgccctcggc
      541 ctggatcagg cgggccgttt cctcggcgtc ctcggctttg ctggcgaagt tgacgacgat
      601 caggccagcg ccccgtcttg cggtttccac cgcgatcgcc cggccaagcc ccgttgaagc
      661 gccggtgacc accaccgtga agccttcaaa atcccgtccc aacatgcgcc tcttccccat
      721 tcgaacatgt gtttggagtt ttgtagcgac aggacgcccg cttgtctcgc ggtcgagacg
      781 ggcctaaatc gcagcgatga cagacctgaa cgtcacccag ctcggccgca tggtcgacgc
      841 gcccgccagc cccgagctgg ctgttctcga acgtgtgccc aatccgcaag cggacgtgct
      901 ctatctgtcg cgcttcgtgg ctcccgagtt cacctcgctg tgcccggtca ctggccagcc
      961 cgactttgcg cacctggtca tcgactacgc cccgcgcgac tggctgatcg aaagcaagtc
     1021 gctgaagctg tatctggcca gcttccgcaa tcacggctcg ttccacgagg actgcacggt
     1081 caaggtggcg cgcaagatcg tcgagatcgc cgacccgcac tggctgcgca tcggcggcta
     1141 ctggtatccg cgcggcggca tcccgattga tgtgttctgg cagacgggcg tcgccccgga
     1201 aggcctctgg gtgcccgacc agggcgtcgc gccgtatcgc ggccgcggct aggccttcga
     1261 cagccgtcgc cggttggcgc ggaccttctt gcgcaggtgg ctgatcgacc ccgccgccac
     1321 ggctggaccg ttggtcccga gctgaccggt catggccagc atcggcaggg tcatggccgc
     1381 cgtcattttc tcgccgacca tcagatcgat ctcggcctga gccgccgccc cgcccgccgc
     1441 aatcttcatc gtgcgcagac cgatcacggt cgacgcctcc aggcccatgg cccaggcgtt
     1501 ccaggccagg ccggcccagg ggtccttgcg tcgcgccatc gtcgtctccg tccgctgtgt
     1561 ttcaggacaa cgcgcgggaa gcggctttgg cgctagccgg gcgcccattc actggtgatc
     1621 gccgcgacgt ccggtcgaac acgctcctgc ggctgctcac tgcatgtgcc cagatagagg
     1681 aagccggcga cctgctcacc cgcgccgacg ccaaggatcg ccagcgcgcg gggatcgtaa
     1741 gcgtaccagt cagtgatcca gttggcgccc cagcccatgg cggatgcggc cagcagcatc
     1801 tgcatgcaca ccgccgaggc gctctgacgt tgttcccact cggggatttc accagggatg
     1861 tagcgcgaaa tcaccgccac gcagaccggc ggccgcgtca gcttgcgcag ggcggcgttg
     1921 gccttgaccg gattggcctg atcgctcgcc agtgcggtga tcttgtcggc gaaaacgtcc
     1981 ttggccgggc cgcgcaggat gatgaaccgc cagggcgaca gcttgccatg atcgggcaca
     2041 cgggccgcca gccgcagcag gtcggcgagc tggccatcgt cagggcctgg cgcggtcagg
     2101 gtcatggccg aagccgagcg acgccgcgcc agaaagctca gcacctctgg cgcagtctc
//
LOCUS       DAOMEO010000551         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5365_length_2159_cov_2.267586, whole genome shotgun sequence.
ACCESSION   DAOMEO010000551 DAOMEO010000000
VERSION     DAOMEO010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_2159_cov_2.267586"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="PLH31_12115"
     CDS             complement(<1..1739)
                     /locus_tag="PLH31_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQR89977.1"
                     /translation="MTYQAPVRDHVFILRDMLNIDQHGQLPAFADASFDTVEQILEAA
                     AQFTGEVLAPLNTVGDKVGCVLDATTNEVTTPPGFKEAYKQLCEGGWTGLGSDPAYGG
                     QGLPHVVNLAFSEMSSSANMAFSMYPGLAHGAYSAIHEGGTDAQKELYLPKMVTGEWT
                     GTMNLTEPHCGTDLGLLRTKAVPQADGSYKITGQKIWISAGEHDMADNIIHLVLARIE
                     GAPAGVKGISLFIVPKFLPKDDGSVGERNVGAKCVGLEEKMGIHGNATCVMQYDDAKG
                     FLIGEENAGLKIMFVMMNEARLGVGMQGVAQGEAAYQAAAAFAKDRLQGRSLTGPKNA
                     DGPADPIIVHPDVRRMLLESRALIEGGRAFLFWTALHGDLAHAHPDEKVREKAEDYMG
                     LMTPVLKGYLTDKGFKVCSDAVQVHGGSGFTEHFPVSQFMRDCRIALIYEGTNGVQAL
                     DLVGRKLASKGGRAVMTFFQEIDQFIGENDTDEGLKPFIKALAEVKAQLQDGTMWLMQ
                     NGLQNPDNAGAASTDYMHLFGLTGLAYMWALQVKAAQAKIAAGSSDPFYSTKVVTAQY
                     FIERILPDAGAHLT"
     gene            complement(1784..>2159)
                     /locus_tag="PLH31_12120"
     CDS             complement(1784..>2159)
                     /locus_tag="PLH31_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR89978.1"
                     /translation="LNPAREGLNRVYSHKDRVRLQLILRGKRVGLSLSEIRELLDLYD
                     ENDDGAAQMARSLKKFRERATALEQQREDIDGALIELHQACARLEQRLAEIRPDLLPR
                     AADYDQVLRARLDGHADAALGK"
BASE COUNT          334 a          682 c          744 g          399 t
ORIGIN      
        1 gtcagatgcg cccctgcgtc aggcaagatg cgctcgatga aatattgcgc cgtgacgacc
       61 ttggtcgaat agaacgggtc ggacgagccg gccgcgatct tggcctgggc cgccttgacc
      121 tgcagggccc acatgtaggc caggcccgtc aggccgaaga ggtgcatgta gtcggtggag
      181 gccgcgccgg cgttgtcggg gttctgcagg ccgttctgca tcagccacat ggtgccgtcc
      241 tgcagctggg ccttcacctc ggccagggcc ttgatgaagg gtttcaggcc ttcgtcggtg
      301 tcgttctcgc cgatgaactg gtcaatttcc tggaagaagg tcatcaccgc gcggccgccc
      361 ttggaggcca gcttgcggcc caccagatcc agggcctgca cgccattggt gccttcatag
      421 atcagggcga tgcggcaatc gcgcatgaac tggctgaccg ggaagtgctc ggtaaagccg
      481 ctgccgccgt gcacctgcac cgcgtcggaa cagaccttga agcccttgtc ggtcaggtag
      541 cctttcagca ccggcgtcat caggcccata tagtcctcgg ccttttcgcg caccttttca
      601 tcggggtggg cgtgggccag gtcgccgtgc agggcggtcc agaacaggaa ggcgcggccg
      661 ccctcgatca gggcccggct ctccagcagc atgcgacgca cgtcggggtg gacgatgatc
      721 gggtcggccg ggccgtcggc gttcttgggg ccggtcagcg agcggccctg caggcgatcc
      781 ttggcgaagg cggcggcggc ctgataggcg gcctcgccct gggccacgcc ctgcatgccg
      841 acgcccagcc gggcctcgtt catcatcacg aacatgatct tcaggccggc gttttcctcg
      901 ccgatcagga agcccttggc atcgtcgtac tgcatcacgc aggtggcgtt gccgtggatg
      961 cccatctttt cttccaggcc cacgcacttg gcgccgacat tgcgctcgcc caccgagccg
     1021 tcgtccttgg gcaggaactt gggcacgatg aacaggctga tgcccttcac cccggccggc
     1081 gcgccttcga tgcgggccag caccagatgg atgatgttgt cggccatgtc gtgctcgccg
     1141 gccgagatcc agatcttctg gccggtgatc ttgtaggacc cgtcagcctg gggaaccgcc
     1201 ttggtgcgca gcaggcccag gtcggtgccg caatggggct cggtcaggtt catggtgccg
     1261 gtccattcgc cggtcaccat cttgggcaga tacagttcct tctgggcgtc cgtgccgccc
     1321 tcatggatgg ccgaataggc gccgtgggcc aggcccggat acatcgagaa ggccatgttg
     1381 gccgagctcg acatctcgct gaacgccaga ttcacgacgt gcggcaggcc ctggcctcca
     1441 taggcggggt ctgagcccag gcccgtccag ccgccttcgc acagctgctt gtaggcctcc
     1501 ttgaagccgg gcggggtggt gacctcattg gtcgtcgcgt ccagtacgca gccgaccttg
     1561 tcgcccacgg tgttcagcgg ggccaggacc tcgccggtga actgggcggc ggcttccagg
     1621 atctgctcga cggtgtcgaa cgaggcgtcg gcgaaggccg gcagctggcc atgctgatcg
     1681 atgttcagca tgtcgcgcag gatgaagacg tgatcgcgaa cgggcgcctg gtaggtcatg
     1741 aaaggtcctg gaaaataggg gcgtataaga gagaggggga agattacttg cccagtgcgg
     1801 cgtcggcgtg accgtccagc cgggcgcgca gcacctggtc atagtcggcg gcgcggggca
     1861 gcaggtcggg gcggatctcg gccagacgct gttcaaggcg ggcgcaggcc tggtgcagct
     1921 cgatcagggc gccgtcgatg tcttcgcgct gctgctccag cgcggtggcg cgctcgcgga
     1981 acttcttcag cgaccgggcc atctgggccg cgccgtcgtc gttctcgtca tacaggtcca
     2041 gcagttcgcg gatttccgac agcgacagcc ccacgcgctt gccgcgcagg atcagctgca
     2101 ggcggacgcg atccttgtgg gaatagacgc ggttgaggcc ctcgcgggcc ggattgagc
//
LOCUS       DAOMEO010000552         2156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5371_length_2156_cov_2.770585, whole genome shotgun sequence.
ACCESSION   DAOMEO010000552 DAOMEO010000000
VERSION     DAOMEO010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5371_length_2156_cov_2.770585"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..>2156
                     /gene="katG"
                     /locus_tag="PLH31_12125"
     CDS             332..>2156
                     /gene="katG"
                     /locus_tag="PLH31_12125"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQR89979.1"
                     /translation="MDGSDIVEPTSKCPLKHGVRFHTSFGGRSNRDWWPNQLNLKILS
                     QHSSLVSPFSPTFRYAEQVEKLDVEALKKDLAALMTDSQDWWPADYGHYGPFFVRMAW
                     HSAGTYRTGDGRGGAGAGQQRFAPLNSWPDNGNLDKARRLIWPIKQKYGNQISWADLM
                     ILAADVGMETMGFKTFGFGFGREDTWEPEEDVHWGAEDTWLGDDRYKGERQLDKPLAA
                     VQMGLIYVNPEGPNGNPDPLASAHDIRETFARMAMNDEETVALIAGGHTFGKAHGAGD
                     VAHVGPEPEGAGIVEQGLGWRNSWESGKGVHTITSGLEGAWKPNPIKWDNGYFDTLFG
                     HEWELTKSPAGAHQWKPKDPAAQTLVPDAHDPSKRHAPMMATTDLALRFDPIYGPISK
                     RFHENPDQFQDAFARAWFKLTHRDMGPIARYRGPLVPKEELIWQDPVPAVDHPLVNAA
                     DIRDLKARVLASGLSVSQLVATAWASASTFRGSDKRGGANGARIRLEPQRNWAVNQPA
                     QLATVLATLEGIQAAFNASQTGGKKVSLADLIVLAGSAGVELAAKAAGHEVEVAFTPG
                     RTDASQAQTDIESFSVLEPTADGFRNYLKTQYTLTAEELLVD"
BASE COUNT          410 a          719 c          681 g          346 t
ORIGIN      
        1 agattcatta caggtagcct atcgcatcca ttggacttta gcgcttctct ttatcgcatg
       61 aatggccaaa actagcttgg caagacggtc cgccagagag cgtcacaaca aagggttacc
      121 tgggagcagc gcaggaacac gacatcaatc tggatgacaa cacgcccgtc gccggtgtca
      181 gcagggccgc acctcaggtg cgtctccggc tgggcgccgc ccttggaggg cgcgtgtctt
      241 cccccgtcag gaagcgggtc tgtcttccgc catcgtcgcg gccttcatcc gggggccacg
      301 tcagccacag cagccaagat cggagccagt tatggacgga agcgacatcg tcgaacccac
      361 gagcaaatgt cccctcaagc atggggtgcg gttccacacc agtttcggag gccggtcgaa
      421 ccgtgactgg tggcccaacc agctgaacct gaagattctc tcgcagcact cgtcgctggt
      481 cagcccgttc tccccaacct tccgctatgc cgagcaggtc gagaagctgg atgtcgaggc
      541 cctgaaaaag gacctcgcgg cgctgatgac cgactcccag gactggtggc ccgccgacta
      601 tggccactat ggtccgttct tcgttcgcat ggcctggcat agcgccggca cctatcgcac
      661 cggcgatggt cgcggaggcg cgggcgcggg ccagcagcgc tttgcgccgc tcaacagctg
      721 gcccgacaac ggcaacctcg acaaggcccg ccgcctgatc tggccgatca agcagaagta
      781 cggcaaccag atcagctggg ccgacctgat gatcctggcc gccgacgtcg gcatggagac
      841 catgggcttc aagaccttcg gcttcggctt tggacgggaa gacacctggg agcccgagga
      901 agacgtccac tggggcgccg aggacacctg gctgggcgac gaccgttaca agggtgagcg
      961 ccagctcgac aagccgctgg ccgctgtcca gatgggcctc atctacgtca atcccgaagg
     1021 ccccaacggc aatcctgatc ctctggcctc ggcccacgac atccgcgaaa ccttcgcgcg
     1081 catggccatg aatgacgagg agacagtagc cctgatcgcg ggcggtcata ccttcggcaa
     1141 ggcccacggc gcgggcgacg tggcgcatgt cgggccggag cccgaaggcg ccggcattgt
     1201 cgagcagggt ctgggctggc gcaacagctg ggagagcggc aagggcgttc acaccatcac
     1261 cagcggcctg gaaggcgcct ggaagcccaa tcccatcaag tgggacaacg gctatttcga
     1321 cacgctgttc ggccatgagt gggagctgac caagagccct gccggggccc atcaatggaa
     1381 gcccaaggat ccagcggccc agaccctggt gcccgacgcc cacgacccgt ccaagcgcca
     1441 cgcgcccatg atggcgacaa cggacctggc gcttcggttc gaccccatct acgggccgat
     1501 ctccaagcgc ttccacgaga acccggacca gttccaggac gcattcgccc gcgcctggtt
     1561 caagctgacc caccgcgaca tgggcccgat cgcccgttat cgcggaccgc tggtgcccaa
     1621 ggaagagctg atctggcagg atccggttcc ggccgtggat catccgctgg tcaatgcggc
     1681 cgatattcgc gacctgaagg ccagggtgct agcctcgggc ctgtcggtct cgcagctggt
     1741 tgcaacggct tgggcctcgg cctcgacctt ccggggttcg gacaagcgcg gcggcgccaa
     1801 cggcgcccgc attcgcctgg agccgcagcg taactgggcg gtcaaccagc ctgctcaact
     1861 cgcgaccgtg ctggcgaccc tggaaggcat ccaggcggcg ttcaacgcca gccagacggg
     1921 cggcaagaag gtgtctctgg ccgatctcat cgtgctggcg ggctctgcgg gcgtcgaact
     1981 ggcggccaag gctgctggcc atgaggtgga ggttgccttt acgccgggtc gcaccgacgc
     2041 ctcccaggcc caaaccgaca tcgagtcgtt ctcggtgctt gagccgaccg ccgacgggtt
     2101 ccgcaactat ctgaagacgc aatacaccct gacggccgag gaactgctgg tcgacc
//
LOCUS       DAOMEO010000553         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5379_length_2154_cov_2.473082, whole genome shotgun sequence.
ACCESSION   DAOMEO010000553 DAOMEO010000000
VERSION     DAOMEO010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5379_length_2154_cov_2.473082"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..590
                     /locus_tag="PLH31_12130"
     CDS             129..590
                     /locus_tag="PLH31_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQR89980.1"
                     /translation="MSKIDDRLNDLGVVLPQPVAPVANYVPFVRSGNLVHISGQISID
                     ASGGIKGTVGVDVDLETAQKAARLCGVNLLAQMKAACDGDLDRVVRVVKLGGFVQAGP
                     DFIDIPKVINGCSDLMVEVLGDAGRHARSAVGVYRLPLGFAVEVDAVVEIA"
     gene            590..1363
                     /locus_tag="PLH31_12135"
     CDS             590..1363
                     /locus_tag="PLH31_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HQR89981.1"
                     /translation="MRVHERPSAEVFGEAWERLFEPAIAHRGLWTPGGAPENSLAAFQ
                     AACARGYAIELDVQLTADGEAVVFHDDRLERLTGVEGRLRDHSAADLGAMALMGTDET
                     IPTLADTLTLIGHRAMVYIELKTPFGEVGELEKRVSEVLIDHNGPTAVIGFNPYSHAW
                     FADHHPQILRGLDSYGWNDDSARKLAPEVRKSLAALEQVEIARPDFLALGLDMLPSAR
                     ADVYRAKGMPVIAWTVRSPEQWDAVSDHCDNLIFEGFEA"
     gene            1363..>2154
                     /locus_tag="PLH31_12140"
     CDS             1363..>2154
                     /locus_tag="PLH31_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HQR89982.1"
                     /translation="MSLKAAVRVHREIAEVGREAWDACAATTGDPFVSYDFLSILEES
                     GCVAARTGWAPQHLSIEDETGAVAGVMPLYLKSHSQGEYVFDHSWADAYERAGGRYYP
                     KLQCSSPFSPVTGSRLIVRPDIDADEGRSALLGGALTLCERMGASSLHVTFPRADEWE
                     WMGQRGMLLRQDQQYHWENAGYATFDDFLAALSSNRRKTIRRERRDAQAGLEIVALTG
                     DELTEAHWDAFFGFYMDTGSRKWGRPYLNRAFYSLLGQRMADTVLL"
BASE COUNT          322 a          705 c          735 g          392 t
ORIGIN      
        1 ttgagggccg cgcctggcgc ggttccgaca gatctgattg aaggcccgga ccggcgacgg
       61 ttcgggcctt ttttcttgtg cccgcgccct gcggcgttaa gcattgcggc gcgtgaacgg
      121 agtacctgat gtcgaagatc gatgaccgcc tgaacgacct tggcgtggtg ctgccgcagc
      181 ctgtggctcc cgtcgccaac tatgtgccgt tcgtgcgatc gggaaatctg gtccacatct
      241 cgggccagat ctcgatcgac gcgtcgggcg ggatcaaggg cacggtcggc gttgatgtcg
      301 acctggagac cgcccagaag gcggctcgcc tctgtggcgt gaacctgctg gcccagatga
      361 aggccgcctg cgacggcgac ctggatcgcg tcgttcgcgt ggtcaagctg ggcggctttg
      421 ttcaggccgg acccgacttt atcgacattc ccaaggtgat caatggctgc tcggacctga
      481 tggtcgaggt gctgggcgat gctggccgtc acgctcgctc ggcggtgggc gtctatcgcc
      541 tgccgctggg ctttgcggtc gaggtcgatg cggtggtgga gatcgcctga tgcgcgttca
      601 tgagcgtccg tcggccgagg tgtttggcga ggcctgggag cgcctgttcg agcccgccat
      661 cgcccaccgc ggtctgtgga cgcccggcgg cgcccctgaa aattcgctgg ccgcctttca
      721 ggccgcctgc gcgcgcggtt acgccatcga gcttgatgtc cagctgacgg ccgacggtga
      781 ggccgtggtc ttccatgacg accgtctgga acgcctgacg ggcgtcgaag ggcgcctgcg
      841 cgatcacagc gcggccgatc tgggcgccat ggccctgatg gggaccgacg agaccatccc
      901 gaccctggcc gacaccctga ccctgatcgg tcaccgggcc atggtctata tcgagctgaa
      961 gaccccgttc ggcgaggtcg gcgagctgga gaagcgggtc tccgaggtgc tgatcgatca
     1021 caatggcccc accgccgtga tcggcttcaa tccctattcc cacgcctggt ttgccgacca
     1081 ccatccccag atcctgcggg ggctcgactc ctacggctgg aacgacgaca gcgcccgcaa
     1141 gctggcgccg gaggttcgca agtcgctggc cgccctggag caggtcgaga tcgcgcgccc
     1201 ggattttctc gcccttggcc tcgacatgct gcccagcgcc cgcgccgatg tctatcgcgc
     1261 caagggcatg ccggtgatcg cctggacagt gcgttcaccc gaacagtggg acgcggtcag
     1321 cgatcactgt gacaacctga tcttcgaggg tttcgaggcg tgatgtcgct caaggcggcg
     1381 gttcgcgtcc atcgcgagat cgccgaggtc ggacgcgagg cctgggacgc gtgcgccgca
     1441 acgacggggg acccgttcgt cagctacgac tttctgtcga tcctggagga gagcggctgc
     1501 gtcgccgccc gcaccggctg ggccccgcaa cacctgtcga tcgaggacga gaccggcgcc
     1561 gtggcggggg tcatgccgct gtatctgaag tcccatagcc agggcgaata cgtcttcgac
     1621 cattcctggg ccgacgccta tgagcgggcc ggagggcggt actatcccaa actccagtgc
     1681 tcttccccgt tctcgccggt gaccggttcg cggctcatcg tgcggcccga tatcgacgct
     1741 gatgagggcc gctcggccct gctgggcggg gccctgaccc tgtgcgagcg catgggggcc
     1801 tcgtcgctgc acgtgacctt ccccagggcc gatgaatggg aatggatggg ccagcgcggc
     1861 atgctgctgc ggcaggacca gcagtatcac tgggaaaacg ccggctacgc caccttcgac
     1921 gacttcctgg cggccctgtc gtccaaccgc cgcaagacca tccgccgcga gcgccgcgac
     1981 gcgcaagccg gtctggagat cgtggccctg accggcgatg agctgaccga ggcgcactgg
     2041 gacgctttct ttggcttcta catggacacc ggcagccgca agtggggccg cccctatctg
     2101 aaccgggcct tctattcgct gctgggccag cggatggccg acacggtgct gctg
//
LOCUS       DAOMEO010000554         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5387_length_2152_cov_2.376729, whole genome shotgun sequence.
ACCESSION   DAOMEO010000554 DAOMEO010000000
VERSION     DAOMEO010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_2152_cov_2.376729"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..703
                     /locus_tag="PLH31_12145"
     CDS             128..703
                     /locus_tag="PLH31_12145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQR89983.1"
                     /translation="MKGPAFALFDTLIGRCGVAWSDSGLIGMQLPEATPGAAWARLRK
                     RFPEAIETPPPPEIERVIERVRDLLAGGRDDLTDIVIDQSQVQGFNWRVYEIARAIAP
                     GETSTYGEVARAMGEPGAARAVGKALGENPWPIVVPCHRVLAASGGMGGFTAEGGTST
                     KAQLLTIERARTSAAPTLFDLEFSVAPPRGG"
     gene            complement(856..1305)
                     /locus_tag="PLH31_12150"
     CDS             complement(856..1305)
                     /locus_tag="PLH31_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89984.1"
                     /translation="MKEHSSMRLIALALTVAGLATTAGMSLAAPSTPNTILPGHWEYS
                     YKVGPIPAGDETRCLKQSDVEQFAKGICTRKYTCDYETRVVKDGKIALKGTWTDKKGR
                     VAPVTAKGGYTAESFQLDVKMKTVNGIPLAGVMDARRVGATCPADAK"
     gene            1515..1796
                     /gene="rpmB"
                     /locus_tag="PLH31_12155"
     CDS             1515..1796
                     /gene="rpmB"
                     /locus_tag="PLH31_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQR89985.1"
                     /translation="MSRRCELTGIGPMVGHNVSHSNIKTKRRFLPALSPATLASDSLG
                     QTFRLRISNAALRTLDFKGGQDEQLSPRALKIKAQVKAKAKAAAVAVAA"
     gene            complement(1955..>2152)
                     /locus_tag="PLH31_12160"
     CDS             complement(1955..>2152)
                     /locus_tag="PLH31_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89986.1"
                     /translation="AGKSAEQVRAEVVKAASTVCWADLRGEAMAGYLYPACIRASVND
                     AYAKINAPAQKLASNSVVSAR"
BASE COUNT          358 a          709 c          724 g          361 t
ORIGIN      
        1 gtcgagaggc tgctggcggc cagcaggacg gcgaggacga ggtggcgcac ggcgatctcc
       61 agacagcaag aagcggaaat cgtagggcgc cgctcgaaag ccgcaaccgc gatgctataa
      121 atgagggatg aaggggccag ctttcgcgct tttcgacacg ctcattggcc gttgtggcgt
      181 cgcctggagc gattccgggc tgatcggcat gcaactgccc gaggcgacgc ccggcgcggc
      241 ctgggcccgt ctgcgcaagc gattccccga ggcgatcgaa acgccgccgc cgcccgagat
      301 cgagcgggtc atcgaacggg tgcgcgacct gttggccggc ggccgcgacg acctgaccga
      361 tatcgtgatc gaccagagcc aggtccaggg cttcaactgg cgggtctatg agatcgcccg
      421 ggctatcgcc cccggcgaga cctcgaccta tggcgaggtg gcccgcgcca tgggcgagcc
      481 cggcgccgcg cgggcggtgg gcaaggcctt gggcgagaac ccctggccca tcgtcgtgcc
      541 ctgccaccgc gtgctggccg cgtccggcgg aatgggcggc ttcacggctg agggcgggac
      601 cagcaccaag gcccaactgc tgaccatcga gcgggcgcga accagcgcgg cgcccacctt
      661 gtttgacctg gaattctcgg tggccccgcc gcgcggcggc tagggccacg cgcaggttaa
      721 cgccgcgagc ggaacacgcg aacaggggcg aaacgaatca tgaccgaatc agcgacgtcg
      781 gccgattcag tctttgttcc ctacaaccca ttgtacctta attcggatta accacaaacg
      841 gtccgcgatg agccgctatt tggcgtcggc cgggcaggtg gcccctacgc gcctggcgtc
      901 catcacgccc gccaggggga ttccgttgac ggtcttcatc ttgacgtcca gctggaagct
      961 ctcggctgtg tagccgccct tggcggtcac cggggccacg cgacccttct tgtcggtcca
     1021 ggtgcccttg agcgcgatct tgccgtcctt caccacgcgg gtctcgtagt cgcaggtata
     1081 tttgcgggtg cagatgccct tggcgaactg ctcgacgtcc gactgcttga ggcagcgcgt
     1141 ctcgtcgccg gccgggatgg ggccgacctt gtagctgtac tcccagtgcc ccgggagaat
     1201 cgtgttcggc gtggacggcg cggccaggct catgccggcg gtcgtggcga gacctgcgac
     1261 ggtcagggcg agggcgatca ggcgcatgga cgagtgttcc ttcatgggct ctctatatag
     1321 ggcggcagat gaacctggct tgaaggcggt cttgaggcgc gcttcacctg ccaggcggtc
     1381 ggaggcggtc atcgcgcctt gactcggcaa gcccccttcg cctatatcgc ccgctctcgc
     1441 ggagtggcta gcccttcgcg ccccgaattc aatgcatgcg gagccctcgg gtttcgccca
     1501 gccaaggtaa tcccatgtcg cgccgctgcg aactcaccgg tatcggtccg atggttggcc
     1561 acaatgtgag ccactccaac atcaagacca agcgccgctt cctgccggcc ctgtcgccgg
     1621 cgacgctggc ctcggattcg ctgggccaga ccttccgtct gcgcatcagc aacgccgccc
     1681 tgcgcaccct cgacttcaag ggcggccagg acgagcagct gtcgccgcgc gccctgaaga
     1741 tcaaggctca ggtcaaggcc aaggccaagg ccgccgccgt ggctgtcgcc gcctaagcga
     1801 cgatccagcg cacaatcgaa aagcccgcac ggagcgatcc gcgcgggctt tttcgcgtct
     1861 ggagtccggc ctctgaaatc tcgatcagac aaagcaaagc ccggcgcgga ggaccgcgcc
     1921 gggcttggta agcttactga aggatcaggc ctgtttagcg ggccgagacc accgagttcg
     1981 aagccagctt ctgggccggg gcgttgatct tggcataggc gtcgttgacc gaagcgcgga
     2041 tgcaggcggg gtacaggtag ccagccatag cttcgccgcg caggtcagcc cagcacacgg
     2101 tgctggcggc cttgacgact tcggcgcgga cctgttcggc cgacttgccg gc
//
LOCUS       DAOMEO010000555         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5394_length_2150_cov_1.972792, whole genome shotgun sequence.
ACCESSION   DAOMEO010000555 DAOMEO010000000
VERSION     DAOMEO010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5394_length_2150_cov_1.972792"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLH31_12165"
     CDS             complement(<1..1283)
                     /locus_tag="PLH31_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQR89987.1"
                     /translation="MNSVRGRLVLLTISLLVPALLSMGFLLAGADRQGRAQLHEQMLA
                     TARALSGATDRQAAVGIAVAETLTTDQALLAGDGAAFHARARRVTDQRSGWVVVTDST
                     GQQIVNTLRPYGTPLPVTPRPEQLANAYRMGLSKVSDLRMGIMARKPVITVGTPVTIR
                     GKPYVLAYVVEASSFTSVFRQQRVPDRWVATLLDNNRKVIARSIRHEKWVGHSASADM
                     TENLRRSNEGVNRSKSLDGVPTMVAYTRSPQTGWTLVVAIPRADMARTVTRSVVAATG
                     VFLLLLVLGIGLALFFSRGINREVGRLVSAAHAIGQGQPVVAPAEPGLEEIAAIHVAL
                     SDASGELQTRAERQKVMINELNHRVKNTLATVQALARQTFGKLEGAAVEVFTERLIAL
                     SGAHDLLTRGGWSKADLGELMQVCTRAHGERVECVG"
     gene            1483..2100
                     /gene="pgsA"
                     /locus_tag="PLH31_12170"
     CDS             1483..2100
                     /gene="pgsA"
                     /locus_tag="PLH31_12170"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQR89988.1"
                     /translation="MKNLPNILTASRLVLALFMFVALAAAAGAVPWVSERLTAEDQFR
                     LERWAFWAFVVAAVTDFFDGWLARKMDAVTVWGAILDPIGDKILVCGALLGLMALGPN
                     AMIVLPAGLILFREFAVSALREVGAGKGIKLPVTVLAKWKTTLQLVAIGAEMVLASWA
                     AFGLPDTPDVVGPATLAIHGLLWLAAIITLITGAQYWEAARKALT"
BASE COUNT          336 a          748 c          702 g          364 t
ORIGIN      
        1 cccacacatt cgacccgctc gccgtgggcg cgggtgcaga cctgcatcag ttcgcccagg
       61 tccgccttgc tccagccgcc ccgggtcagc aagtcatggg cgcccgagag cgcgatcaat
      121 cgctcggtga acacctcgac cgccgcgccc tccagcttgc cgaaggtctg gcgcgccagg
      181 gcctgaaccg tggccagggt attcttcacc cgatggttca actcgttgat catcaccttc
      241 tgccgctcgg cgcgggtctg cagttcgccg gaggcgtcgc tcagggccac gtggatagcg
      301 gcgatctctt cgagcccggg ctcggccggc gcgaccacag gctggccctg gccgatggcg
      361 tgagcggccg agaccagtcg cccgacctcg cgattgatcc cgcgagagaa gaacagcgcc
      421 agacctatgc ccagcaccag cagcagcagg aagaccccgg tcgccgcgac caccgaacgg
      481 gtcaccgtgc gcgccatgtc ggcgcgggga attgccacca ccagcgtcca gcccgtctgg
      541 ggcgagcggg tgtaggcgac catggtcgga acgccatcca gcgacttgct acgattgacc
      601 ccttcgttgg aacgccggag attttcggtc atgtccgccg acgccgaatg gccgacccat
      661 ttttcatggc ggatcgagcg ggcgatcacc ttgcggttat tgtcgagcaa ggtcgccacc
      721 cagcggtcgg ggacgcgctg ctggcggaaa accgaagtga aggaagacgc ctcgaccaca
      781 taggccagga catagggctt gccccggatc gtgacgggcg tacccaccgt gatgactggc
      841 ttcctggcca ttatgcccat gcgcaggtcc gaaaccttgc tcaggcccat cctgtaggcg
      901 ttggccagct gttccggccg cggcgtcacc ggcaagggcg tgccgtaggg cctcagcgta
      961 ttgacgatct gctggcccgt gctgtcggtg accaccaccc agccactgcg ctggtcggtt
     1021 acccggcggg cccgcgcatg gaacgccgcc ccgtcgccgg ccagcagggc ctggtcggtc
     1081 gtcagggtct ccgccacggc gatgccgacg gcggcctggc ggtcggtggc gccagacagg
     1141 gcccgggcgg tggccagcat ctgctcgtga agctgggcgc ggccctgccg gtcggcgcca
     1201 gccagcagaa aacccatgct cagcagggcc ggaacaagca ggctgatcgt cagcaggaca
     1261 agcctgccgc ggaccgagtt cacccctgcc ctggccatcg cgccataccg gtcaagcaag
     1321 tctcatactc acgcagcgcc atattgacgg ggtcttgtgc gaccctgtgg cctgtatagc
     1381 caatcgtttt cgatccgcct ggcgaaaaat catcgccgcc gtccagggtc ctgcagcgcg
     1441 aaggcgtagc cgtttggggg tttcccgcgt tagaaccgca tcatgaagaa tctgccgaac
     1501 atcctgaccg cctcgcgcct cgtcctggcg ctgttcatgt tcgtggcgct ggccgccgcc
     1561 gccggcgccg tgccgtgggt cagcgagcgc ctgacggccg aggatcagtt ccggctggag
     1621 cgctgggcgt tctgggcgtt cgtggtcgcc gccgtcaccg acttcttcga cggctggctg
     1681 gcgcgcaaga tggacgccgt caccgtgtgg ggcgcgatcc tggacccgat cggcgacaag
     1741 atcctggtct gcggcgccct gctgggtctg atggccctgg gtcccaacgc catgatcgtc
     1801 ctgcccgccg gcctgatcct gttccgcgaa ttcgccgtca gcgccctgcg cgaagtgggc
     1861 gcgggcaagg ggatcaagct gccggtcacc gtgctggcca agtggaagac caccctgcag
     1921 ttggtggcga tcggcgccga gatggtgctg gcctcctggg ccgcctttgg cctgcctgac
     1981 accccagacg tcgtgggccc cgccaccctg gccatccacg gcctgctgtg gctggcggcc
     2041 atcatcaccc tgatcaccgg cgcccagtac tgggaagcgg cccgcaaggc gctgacctag
     2101 ggccaatcga cattcagccg accattgtcc tttattcacc gtcattcccg
//
LOCUS       DAOMEO010000556         2149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5395_length_2149_cov_2.915473, whole genome shotgun sequence.
ACCESSION   DAOMEO010000556 DAOMEO010000000
VERSION     DAOMEO010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5395_length_2149_cov_2.915473"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            573..869
                     /locus_tag="PLH31_12175"
     CDS             573..869
                     /locus_tag="PLH31_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89989.1"
                     /translation="MSYLPSPPAFHSDADGVTVHQLLLAAQDAPSALDAVRQGLADNL
                     AEPCKLTLNPVGQIVEASLRVRHLGDQAARLLARDLAAWPGVSSASVEHQWVRP"
     gene            866..1135
                     /locus_tag="PLH31_12180"
     CDS             866..1135
                     /locus_tag="PLH31_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89990.1"
                     /translation="MNLDEDKFGGVGRRLFLVTTEDTPDALMRVLGVASVQQTRLRAL
                     TATPDGPHLAIRLEIDDQGDDRAASLASRLQACPSVRGVGFGWRA"
     gene            1211..>2149
                     /locus_tag="PLH31_12185"
     CDS             1211..>2149
                     /locus_tag="PLH31_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQR89991.1"
                     /translation="MNRRALLATAALVLAAGSAHAQTAPDLSVTIYNSDLALVEDARD
                     LNLKAGRQRLEFKDVSAAIRPETVSLSAAGVTIIEQNFDYDLLTPDKLMEKAVGQQVR
                     IVRTNPGDGKETTEVATVLAANEGVVLRIGDRVEVLRDDGVPTRVIFDKVPETLRARP
                     TLSVTVDADKAGPRASKLSYLTSGLGWKADYVALFDEKKGALDLQGWITLTNTSGTPF
                     DNARTQLVAGDVNQLNNGGYRRQSTSMQSAGTESGDAERLADYYVYPLPQRTTILANQ
                     SKQVGFLSAQGVSARKVYEIREGSFASDANPSSATVA"
BASE COUNT          383 a          767 c          670 g          329 t
ORIGIN      
        1 cgtggcgggg gttgagaagg ctggcgggaa gctgcggagc taacccaaag tcctccccct
       61 ggcgggggag gtgtcgccgc aggcgacgga gggggaagtg ccgctgagtc actgcggtct
      121 tcccccaccg acctacggtc gcctccccct tcggggggac gacttggccc actgcccaaa
      181 ttgcgggcgc ccgctcgccc gaaaaccaaa acctgcccac aaccgcgacc gatcgcccct
      241 cggcgaggca aggtcttgcg catggcgcat acccacgcta cctctagtga agtcgcaagg
      301 agctactggg tccacatggt ctcgcaactc gccatcaaca tcgtgctcgt gaccggcttt
      361 accgccgtcg cggcgatgtc gtgcgccgcg accatgatgt ggaaccagat taaaaccgtt
      421 ccaattcgga cggggcaacg gcgcgcgaga tagactgaac gcagccgacg gatcgcaaga
      481 tcttcaaaaa gccccgcttc cgaaaggagc ggggcttttt tgttgcgcgt ttcgtcggtc
      541 ctccaaaatc aacctgaggg gacttgagac caatgagcta cctgccatca ccacccgcct
      601 tccacagcga cgccgacggc gtcaccgttc accagctgct gctggccgcc caggacgcgc
      661 ccagtgcgct ggacgccgtt cgccagggcc tggccgacaa cctcgcagaa ccgtgcaagc
      721 tgacgctcaa ccccgtgggc cagatcgtcg aggcgtccct gcgcgtccgc cacctgggcg
      781 accaggccgc ccgtctgttg gcccgcgacc tggccgcctg gcccggcgtc agctcggcca
      841 gcgtcgagca tcagtgggtg cgaccttgaa cctggacgag gacaaatttg gcggagtcgg
      901 gcgccgcctg ttcctcgtca ctaccgaaga cacgcccgac gccctgatgc gggtgttggg
      961 cgtggccagc gtgcagcaga cccgcctgcg cgccctgacc gccacgcccg acggcccgca
     1021 cctggccatt cgtctggaga tcgacgacca gggcgatgac cgcgccgcca gcctggcctc
     1081 gcgccttcag gcctgcccgt cggttcgcgg cgtgggcttc ggctggcggg cctagcgctc
     1141 gcaagcgtca ccgcctcgac atcgccccaa tcacatgtag gtttaaacct gtaaaatggg
     1201 gagatcatcg atgaaccgtc gcgcgcttct ggcgaccgcc gcgctcgtgc tcgctgcggg
     1261 ctcggcccac gcccagaccg ctcccgatct ctcggtcacc atctacaatt ccgacctggc
     1321 cctggtcgaa gacgcccgcg atctgaacct gaaagccggg cgccagcgcc tggagttcaa
     1381 ggacgtctcg gccgccatcc ggcccgagac ggtcagcctg tcggcggccg gcgtgaccat
     1441 catcgagcag aacttcgact acgacctgct gactcccgac aagctgatgg aaaaggcggt
     1501 cggccagcag gtgcgcatcg tccgcaccaa tccgggtgac ggcaaggaga ccaccgaggt
     1561 cgccaccgtg ctggccgcca acgagggcgt ggtcttgagg atcggcgacc gcgtcgaggt
     1621 gctgcgcgac gacggcgtgc ccacccgcgt gatcttcgac aaggtgcccg agaccttgcg
     1681 cgcccgtccg accctgtcgg tcaccgtcga cgccgacaag gccggacctc gcgcctcgaa
     1741 actcagctat ctgacctccg gcctaggctg gaaggccgac tatgtggccc tgttcgacga
     1801 gaagaagggc gccctggacc tgcagggctg gatcaccctg accaacactt ccggcacccc
     1861 gttcgacaac gcccgcaccc agctggtggc cggcgacgtc aaccagctga acaatggcgg
     1921 ctatcgccgg cagagcacgt cgatgcaatc ggccggcacg gagagcggcg acgccgagcg
     1981 tctggccgac tactacgtct atcccctgcc ccagcgcacc acgatcctgg ccaaccagag
     2041 caagcaggtc ggcttcttga gcgcccaggg ggtgagcgcc aggaaggtct atgagatccg
     2101 cgaaggctcg ttcgccagcg acgccaatcc ctcgtcggcc accgtggcc
//
LOCUS       DAOMEO010000557         2146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5404_length_2146_cov_2.653754, whole genome shotgun sequence.
ACCESSION   DAOMEO010000557 DAOMEO010000000
VERSION     DAOMEO010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_2146_cov_2.653754"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /gene="acnA"
                     /locus_tag="PLH31_12190"
     CDS             <1..1418
                     /gene="acnA"
                     /locus_tag="PLH31_12190"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HQR89992.1"
                     /translation="HGDVVIAAITSCTNTSNPSVLIAAGLLAKNAVAKGLKVKPWVKT
                     SLAPGSQVVTDYLLKAGLTKHLDALGFNLVGYGCTTCIGNSGPLPEAISKTISDHDLV
                     ACSVLSGNRNFEGRVNPDVRANYLASPPLVVAYALAGSMKIDLATQPLGQDKKGNDVF
                     LKDIWPSNEDIAVLQRKSVTEKMFAARYGDVFKGDKNWQGIKITGGQTYAWEGDSTYV
                     QNPPYFPNMSMTPTPVTDIIEARVLAVFGDSITTDHISPAGSIKTSSPAGQYLVDQGV
                     QPIDFNGYGARRGNHQVMMRGTFANIRIRNRITPDIEGGVTKHFPSGQVMSIYDAAMK
                     YQEEGRPSVVFAGKEYGTGSSRDWAAKGTNLLGVRAVITETFERIHRSNLVGMGVLPL
                     QFIQDGWQKLELTGEEIVSIRGLQDLAPRKQLIVELYRPSDGRIARFPVRCRIDTPTE
                     LEYFKNGGVLNYVLRNLARAD"
     gene            1564..>2146
                     /locus_tag="PLH31_12195"
     CDS             1564..>2146
                     /locus_tag="PLH31_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HQR89993.1"
                     /translation="MRKAALSLLCLALSGLSATSAWAAKPPVAKLPVVVELFTAQGCS
                     SCGKANQLAADLAQRDGVLALTYSVDYWDYLGWKDTFAKPAFAERQRAYVKKFALRDV
                     PTPQMVVAGRVQASGAKAEAVEDLVKTAARAPTNPPDMQFYGNGRVGVGSGPVPRGGG
                     EVWLIRFDPREQEIAVKRGDNKGQTIVHRNVVRE"
BASE COUNT          378 a          756 c          668 g          344 t
ORIGIN      
        1 gtcacggcga cgtggtcatc gcggccatca cctcgtgcac caacacctcc aacccttcgg
       61 tgctgatcgc cgccggcctg ctggccaaga acgcggtcgc caagggtctg aaggtcaagc
      121 cgtgggtgaa gacctcgctg gcccccggct cgcaggtggt caccgactat ctcctgaagg
      181 ccggcctgac caagcatctc gacgcgctgg gcttcaacct ggtgggttac ggctgcacca
      241 cctgcatcgg caattcgggc ccgctgccgg aagccatcag caagacgatc agcgaccacg
      301 acctggtcgc ctgctcggtg ctgtcgggca accgcaactt cgaaggccgg gtgaaccccg
      361 acgttcgcgc caactacctg gcctcgccgc cgctggtggt ggcctacgcc ctggccggct
      421 cgatgaagat cgatctggcc acccagccgc tgggccagga caagaagggc aatgacgtct
      481 tcctgaagga catctggccc tcgaacgaag acatcgccgt cctgcagcgc aagtcggtca
      541 ccgagaagat gttcgccgcc cgctatggcg acgtcttcaa gggcgacaag aactggcaag
      601 gcatcaagat cacgggcggc cagacctacg cctgggaagg cgactcgacc tacgtccaga
      661 acccgcccta cttcccgaac atgtcgatga cccccacgcc ggtcaccgac atcatcgagg
      721 cccgcgtcct ggccgtgttc ggcgactcga tcaccaccga ccacatcagc ccggccggtt
      781 cgatcaagac ctccagcccg gccggccagt acctggtcga ccagggcgtg cagcccatcg
      841 acttcaacgg ttacggcgcg cgtcgcggca accaccaggt catgatgcgc ggcaccttcg
      901 ccaatatccg catccgcaac cgcatcaccc cggacatcga agggggcgtc accaagcact
      961 tcccgtcggg ccaggtgatg tcgatctacg acgcggccat gaagtaccag gaagagggtc
     1021 gcccctcggt ggtcttcgcc ggcaaggaat acggcactgg cagctcgcgc gactgggccg
     1081 ccaagggcac caacctgctg ggcgtccgcg cggtgatcac cgagaccttc gagcgcatcc
     1141 accgttcgaa cctggtgggc atgggcgttc tgcccctgca gttcatccag gacggctggc
     1201 agaagctgga actgaccggc gaggaaatcg tctcgatccg cggcctgcag gacctggcgc
     1261 cgcgcaagca gctgatcgtc gagctctacc gcccgtcgga cggccgcatc gcccgcttcc
     1321 cggtccgctg ccgcatcgat acgcctaccg agcttgagta tttcaagaac ggcggcgtgc
     1381 tgaactacgt gctgcgcaac ctggcgcgcg ccgactaggc gacgatcccg cgttgaaaga
     1441 gacggcccgc cggagcgatc cggcgggccg ttttcgtttg cgacgcgaac ttttcgcaaa
     1501 gaagtcgccc ttcacggcct cgtgaacggc cttgcaggac gatcttggct taagtgaccc
     1561 gcaatgcgta aggccgccct ttccctgctc tgtctggctc tctcgggcct ttccgccacg
     1621 tccgcctggg ctgcaaagcc tccggtggcc aaattgcccg tggtggtcga gctgttcacc
     1681 gcccagggct gctcgtcctg cggcaaggcc aaccagctgg ccgccgatct ggcccagcgc
     1741 gacggcgtcc tggccctgac ctattcggtc gattactggg actatctggg ctggaaggac
     1801 accttcgcca agcccgcctt cgccgagcgc cagcgggcct atgtcaagaa attcgccctg
     1861 cgcgacgtgc ccacgcccca gatggtggtg gccggccgcg tgcaggcctc gggcgccaag
     1921 gccgaggcgg tcgaggacct ggtcaagacc gccgcccggg ccccgaccaa tccgccggac
     1981 atgcagttct acggcaatgg ccgcgtcggg gtcggctcgg gccccgtccc gcgcggcggc
     2041 ggcgaggtgt ggctgatccg gttcgatccg cgcgagcagg agatcgccgt caagcgcggc
     2101 gacaacaagg gccagaccat cgttcaccgc aatgtggtgc gtgagc
//
LOCUS       DAOMEO010000558         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5408_length_2144_cov_2.922451, whole genome shotgun sequence.
ACCESSION   DAOMEO010000558 DAOMEO010000000
VERSION     DAOMEO010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5408_length_2144_cov_2.922451"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..701
                     /locus_tag="PLH31_12200"
     CDS             90..701
                     /locus_tag="PLH31_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQR89994.1"
                     /translation="MSKVTRRLVAAGLPLAALVAPGLAHARPAPAFTAVDIDGQTRSL
                     AEFKGKTVVLEWVNEGCPYVKKHYSGNMQGLQAAATADGVVWLTIASSAPGKQGHFPD
                     GGSARTWMNARGARPTTLLLDGEGVVATAYKAKTTPHMFVIDKTGAIVYEGAIDDIPS
                     NKVEDLPKARNLVAAALADVKAGRKVAVAYVQPYGCALKYKDA"
     gene            complement(818..1297)
                     /locus_tag="PLH31_12205"
     CDS             complement(818..1297)
                     /locus_tag="PLH31_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR89995.1"
                     /translation="MSCSAVAFSAMLWLASSSAVPVQAAPAVATAPPSVAGEPLFSDI
                     VRRAAGLKAQAEAYRASLGADPAAPAATLKDAETFGAKIAELSALDMKGHLALKERGA
                     VDDLKCILRGISVDLPEKLAAMTAARTIKDQDLALRDMVYLLNDNVEVITAPPQPAA"
     gene            1406..>2144
                     /locus_tag="PLH31_12210"
     CDS             1406..>2144
                     /locus_tag="PLH31_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQR89996.1"
                     /translation="MADLDAAWTRLEAAARAAGDKRIVEMFDAEPDRLANLTLDVAGL
                     HIDLSKQAWDEAGLEAALDLAHAADVEGARARMLAGEAINSSEGRAVLHTALRAPATA
                     DIKAGGVPVMADVEAVRIRMKSFAEAVRSGQIKGATGKPFKAILHIGIGGSDLGPRLL
                     WDALRPVKPTIDLRFVANVDGAEFLLTTADLDPEETLVIIVSKTFTTQETLSNANAAR
                     AWLADALGEAAAGQHLAAISTALDKTAA"
BASE COUNT          353 a          747 c          716 g          328 t
ORIGIN      
        1 gaaggccgcc agctaggggt ttccagacaa cgtcaggatg tgagaggcta gccctgcgag
       61 ggggaccaag ccttttctgg agctcgccta tgtccaaagt cacacgccgt ctcgtcgccg
      121 ccggtctgcc tctggcggcg ctggttgcgc ccggcctggc ccatgcccgg cccgcgcccg
      181 ccttcaccgc cgtcgatatc gacggccaga cccggtctct ggccgagttc aagggcaaga
      241 ccgtcgtcct cgaatgggtc aacgagggct gcccctatgt taagaagcac tattcgggca
      301 acatgcaggg cctgcaggcg gccgccaccg ccgatggcgt ggtctggctg accatcgcct
      361 cctcggcccc cggcaagcag ggccactttc cggacggcgg cagcgccagg acctggatga
      421 acgcccgggg cgccaggccc acgaccctgc tgctcgacgg cgagggtgtg gtggccaccg
      481 cctacaaggc caagaccacc ccccacatgt tcgtcatcga taagaccggg gcgattgtct
      541 acgagggcgc catcgacgac atccccagca acaaggtcga ggacctgccg aaggccagga
      601 acctggtcgc cgccgccctg gccgacgtga aggcgggccg aaaggtggcc gtggcctatg
      661 tccagcccta tggctgcgcc ctgaagtaca aggacgctta gggccgggcg tccttcgagg
      721 ctcgcctgcg gctcgcacct caggatgagg tcggttgttc tgaagccctc attctgagac
      781 gcccgcttgc gggcctcgaa ggacgcaggg tgactgccta ggccgccggc tgcggcggcg
      841 cggtgatcac ttcgacattg tcgttcagca gatagaccat gtcgcgcagg gccaggtcct
      901 ggtccttgat ggtcctggcg gcggtcatgg cggccagctt ttcgggcagg tcgaccgaga
      961 tgccgcgcag gatgcatttg aggtcatcga ccgcgccgcg ttccttcagg gccagatggc
     1021 ccttcatgtc gagggcggaa agctcggcga tcttcgcgcc aaaggtctcg gcgtctttca
     1081 acgttgcggc cggggcggcg gggtctgcgc ccagcgatgc gcggtaggcc tcggcctggg
     1141 ccttcagccc ggcggcgcgg cgcacgatgt cggaaaacag cggctcgccg gccaccgagg
     1201 gcggtgcggt ggctaccgct ggcgcggcct ggacgggaac ggcgctgctc gaagcgagcc
     1261 agagcatggc ggaaaaggcg acggcgctgc aggacatggg actctccccg cgaaaaacgt
     1321 cattggtctt catgacgccg cggcatccgt ttggtaagcc cgcgccggta aacgccgatt
     1381 taccaaaccc gcaaggaatg cacgcatggc cgatctcgac gccgcctgga cccgcctgga
     1441 agccgccgcc agggccgctg gcgacaagcg catcgtcgag atgttcgacg ccgagcctga
     1501 ccgcctcgcc aatctgacgc tggacgtcgc cggcctgcat atcgacctgt cgaaacaggc
     1561 ctgggacgag gcgggcctgg aagccgccct cgacctggcc cacgccgccg acgtggaggg
     1621 cgcaagagcc cggatgctgg ccggcgaggc gatcaattcc tccgaaggcc gcgccgtgct
     1681 gcacaccgcc ctgcgggccc ccgccaccgc cgatatcaag gccggcggcg ttccggtgat
     1741 ggccgatgtc gaggccgtcc gtatccggat gaaatccttc gccgaggccg ttcgctcggg
     1801 ccagatcaag ggcgccaccg gcaagccgtt caaggccatc ctgcacatcg gcatcggcgg
     1861 cagcgacctg ggcccccgcc tgctgtggga tgccctgcgc ccggtgaagc cgaccatcga
     1921 cctgcgcttc gtggccaatg tcgacggcgc cgagttcctg ctgaccacgg ccgaccttga
     1981 tccggaagag accctggtca tcattgtctc caagaccttc accacccagg agaccctgtc
     2041 caacgccaac gccgcgcggg cctggctggc cgatgccctg ggcgaggccg ccgccggcca
     2101 gcacctggcc gccatctcca cggcgctgga caagaccgcc gcct
//
LOCUS       DAOMEO010000559         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5425_length_2139_cov_1.953455, whole genome shotgun sequence.
ACCESSION   DAOMEO010000559 DAOMEO010000000
VERSION     DAOMEO010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_2139_cov_1.953455"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1061
                     /locus_tag="PLH31_12215"
     CDS             120..1061
                     /locus_tag="PLH31_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR89997.1"
                     /translation="MSSPIPDPARDHAADIVRAALGDPRQRPVVRSFFDEATFTVSHV
                     VRDPGSDVCAIIDSVLDYDPASGRTAHASADTLIDYVKSEGLTVQWLLETHAHADHLS
                     AAPYLQEKLGGQLAIGHEILTVQAVFGKIFNEGTDFRRDGSQFDHLFNDGDRFEIGGL
                     KATVLHVPGHTPACLAYVIGDAVFPGDTMFMPDYGTARCDFPGGDAGTLYRSIHRLTS
                     LPDEARVFLCHDYKAPPGRTEFAWETTIGAQRTGNIHIRDGVSEDEFVAMRTQRDATL
                     PMPKLILPSVQVNMNGGRPPEPEDNGVRYLKVPLNAL"
     gene            1073..1507
                     /locus_tag="PLH31_12220"
     CDS             1073..1507
                     /locus_tag="PLH31_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQR89998.1"
                     /translation="MLEPLPFVWPLAGGLLVGLSAGLYLLLNGRVAGISGLTAAATGL
                     SKGAPRTLGLLFIAGLLGGAALASALIRRPEVQITSDIWLLVLGGLIVGFGTRWGSGC
                     TSGHGVCGLARLSPRSIVATGVFMAVAALTVFVTRHLLGGLA"
     gene            1504..1923
                     /locus_tag="PLH31_12225"
     CDS             1504..1923
                     /locus_tag="PLH31_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HQR89999.1"
                     /translation="MNRLIVAVLCGLLFGAGMVVSDMINPARVLAFLDVTGTWDPSLA
                     FVMGGALIPSAIAYLIRRRMETPVAAETFHVPESRTIDTPLVAGAVLFGLGWGLVGLC
                     PGPALAALTTGAWQAFVFVGALVAGMALFRLTLDRNA"
     gene            1953..>2139
                     /locus_tag="PLH31_12230"
     CDS             1953..>2139
                     /locus_tag="PLH31_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HQR90000.1"
                     /translation="MDIRPLDEALSASPQIAPEDLPAIAAQGFRSVISNRPDGEEPGQ
                     PSAEALRQAAEAAGLAFA"
BASE COUNT          301 a          806 c          664 g          368 t
ORIGIN      
        1 gtcgatcgtt tccatagccg caagctaagc gcttcccacc gtttccgcta cattaaaata
       61 catacttgca cgaactatga atgggtatat gtgttgagtc accgaaacgg agacacccca
      121 tgtcctcgcc catccccgat cccgcccgcg accacgccgc cgacatcgtg cgcgccgcgc
      181 tgggcgatcc tcgccagcgc cctgtcgtca ggtcattctt cgacgaggcc accttcaccg
      241 tcagccatgt cgtgcgcgat cccggctccg acgtctgcgc catcatcgac agcgtgctcg
      301 actacgatcc ggcgtccggc cgcaccgccc acgcctcggc ggacaccctc attgattacg
      361 tgaaatccga aggtctcacg gtccagtggc tgttggagac ccacgcccac gccgatcacc
      421 tgtcggcggc cccctatctt caggagaagc tcggcggtca gctggccatc ggccatgaga
      481 tcctcaccgt ccaggcggtc ttcggcaaga tattcaacga agggaccgac ttccgccgcg
      541 acggcagcca gttcgaccat ctgttcaacg acggcgaccg gttcgagatc ggcgggctga
      601 aggccacggt cctgcatgtg cccggccaca ccccggcctg tctggcctat gtcatcggcg
      661 acgccgtctt tcccggcgac accatgttca tgcccgacta cggcacggcg cgctgcgact
      721 tccccggcgg cgacgccggg accctgtatc gctcgatcca tcgcctgacc tccctgccgg
      781 acgaggcgcg ggtcttcctg tgccacgact acaaggcgcc gccgggccgg accgagttcg
      841 cctgggagac caccatcggc gcccagcgca ccggcaacat ccatatccgc gacggcgtca
      901 gcgaggacga attcgtggcc atgcgaaccc agcgcgacgc caccctgccc atgcccaagc
      961 tgatcctgcc gtcggttcag gtgaacatga acggcggccg gccgcccgag cccgaggaca
     1021 atggggtgcg ctacctcaag gtcccgctga acgcgctgtg aggcgagccc ccatgcttga
     1081 gcccctcccc ttcgtctggc cgctggccgg cggtctgctg gtcggcctgt cggccggtct
     1141 ttatctgttg ctgaacgggc gcgtggccgg gatctccggc ctgaccgctg cggcgaccgg
     1201 cctgtcgaaa ggcgcgccgc gcaccctggg cctgcttttc atcgccggcc tgctgggcgg
     1261 cgcggccctg gcctcggcgc tgatccgtag gcccgaggtg cagatcacct cggacatctg
     1321 gctgctggtg ctcggcggcc tgatcgtcgg cttcggcacc cgctggggct cgggctgcac
     1381 cagcggccac ggcgtctgcg gcctggcccg cctgtcgccc cgctccatcg tcgccaccgg
     1441 cgttttcatg gcggtcgcgg ccctgaccgt cttcgtcacc cgccacctgc tgggaggcct
     1501 ggcatgaacc ggctgatcgt cgccgtcctg tgcggcctgt tgttcggcgc cggcatggtg
     1561 gtgtccgaca tgatcaaccc ggcgcgcgtc ctggccttcc tggatgtgac gggcacatgg
     1621 gacccttcgc tggccttcgt catgggcggg gcgctgatcc cgtccgccat cgcctatctg
     1681 atccgccggc gcatggagac gccggtcgcg gccgagacct ttcacgtccc cgagagccgg
     1741 accatcgaca cccccctggt cgccggggcc gtcctgttcg gcttgggctg ggggctggtc
     1801 ggcctgtgcc ccggcccggc gctggcggcc ctgacgaccg gcgcctggca ggcctttgtc
     1861 ttcgtcggcg ccctcgtggc cggcatggcg ctgttccgcc tgacgctcga ccgtaacgcc
     1921 tgaaccagaa aagaccctag ggagacaaag ccatggatat ccgccccctg gacgaggccc
     1981 tatccgcctc gccccagatc gccccggaag acctgcccgc catcgcggcc cagggtttcc
     2041 ggtcggtgat ctccaaccga cccgacggcg aggagcccgg ccagcccagc gccgaggctc
     2101 tgcgccaggc cgccgaggcc gccggcctgg ccttcgccc
//
LOCUS       DAOMEO010000560         2133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5440_length_2133_cov_2.564485, whole genome shotgun sequence.
ACCESSION   DAOMEO010000560 DAOMEO010000000
VERSION     DAOMEO010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_2133_cov_2.564485"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1035)
                     /locus_tag="PLH31_12235"
     CDS             complement(79..1035)
                     /locus_tag="PLH31_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQR90001.1"
                     /translation="MSAFDAVSVGRRVLIGEADALRDLSGTLGGSFAKAVETLFAARG
                     RVVCTGIGKSGHVARKIAATMASTGAPAMFVHAAEASHGDLGMIGQDDVVLALSKSGE
                     ARELSDTLAYAKRFSIPLIAITAVADSQLGRAADILLLLPDAPEATAEVNAPTTSTTL
                     QMALGDALAVALLERRGFTASDFRVFHPGGKLGSMLRTVGDLMHGEQELPLAHVDAPM
                     SDALLVMSEKRFGAVGVIDTQGALVGLITDGDLRRHMDGLMSHIAGEVMTRAPLTIGP
                     SALAAEALKIMNERRITVLFVVDGDRPVGILHVHDLLRAGII"
     gene            complement(1032..1265)
                     /locus_tag="PLH31_12240"
     CDS             complement(1032..1265)
                     /locus_tag="PLH31_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90002.1"
                     /translation="MSDRFFMPLMALIAFAMIALALVWPQGYGARSPDPFGSTPIQQT
                     PEMRAKIEREKEGARRRAEEAVRAVKAAGGTVQ"
     gene            1365..2078
                     /gene="gpmA"
                     /locus_tag="PLH31_12245"
     CDS             1365..2078
                     /gene="gpmA"
                     /locus_tag="PLH31_12245"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HQR90003.1"
                     /translation="MPTLVLLRHGQSQWNLENRFTGWVDVDLTAEGEAQARKGGELIK
                     AAGIDLDEAYTSVQTRAIRTCNLALDAAGQSFVPVTKDWRLNERHYGGLTGLNKAETA
                     VLHGEEQVTIWRRSYDIPPPELAAGSEYDFSKDRRYAGKDLPTTESLATTLVRVLPYW
                     EAEIAPKLAAGETILVAAHGNSLRAIVKHLFQVPDADIVHVEIPTGNPLVIELDGALK
                     PVAVRYLDESRAQTLPAIA"
BASE COUNT          363 a          708 c          743 g          319 t
ORIGIN      
        1 agagcgtcgc cttaccgtcg cgacactggt tccgctagcg ctgtcgcatg acaaaggtcg
       61 gcgaaatgta aatcgccgct agataatccc ggcccgaagc aggtcgtgaa catgcagtat
      121 gccgaccggg cgatcgccat cgaccacaaa cagcaccgtg atccgccgct cgttcatgat
      181 tttcagggct tcggcggcca gggccgaggg gccgatggtc agcggcgcgc gggtcatgac
      241 ctcgccggcg atgtggctca tcagcccgtc catgtgacgg cgcaggtcgc cgtcggtgat
      301 caggccgacc agggcgcctt gggtgtcgat gacgccgacc gcgccgaagc gcttctcgct
      361 catcaccagc agggcgtcag acattggcgc gtcgacatgg gccagcggca gttcctgctc
      421 gccatgcatc aagtctccga ccgtgcgcag catcgagccc agcttgccgc cggggtggaa
      481 gacccggaag tcgctggccg taaagccgcg ccgctccagc agggccacgg ccagggcgtc
      541 gcccagggcc atctgcaggg tggtcgaggt ggtgggggcg ttgacctcgg ccgtcgcttc
      601 gggcgcatcg ggcagcagca gcaggatgtc ggcggcgcgc cccagctggc tgtcggccac
      661 ggcggtgatg gcgatcagcg ggatcgagaa gcgcttggca taggccaggg tgtcggacag
      721 ctcgcgcgcc tcgcccgact tggacagcgc cagcaccaca tcgtcctgac cgatcatgcc
      781 caggtcgcca tggctggctt ctgcggcgtg cacgaacatg gccggcgcgc cggtcgaggc
      841 catggtggcg gcgatcttgc gggcgacgtg accggacttg ccgatgccgg tgcagacgac
      901 gcggcccctg gcggcgaaca gggtttcgac ggccttggcg aaagacccgc ccagcgtccc
      961 cgaaaggtcg cgcagggcgt cagcctcgcc gatcagaacg cggcggccga ccgagacggc
     1021 gtcgaaggcg ctcactgtac ggttccgcca gcggccttga cggcgcggac ggcctcttcg
     1081 gcgcggcggc gggcgccttc cttttcgcgc tcgatcttgg cccgcatctc cggcgtctgc
     1141 tggatgggcg tcgagccgaa tggatcgggc gaacgcgccc catagccttg cggccagacc
     1201 agggcgaggg cgatcattgc aaaggcaatc agcgccatca ggggcatgaa gaagcggtcg
     1261 gacatgggcc tgcctatagc atgtgtcgcg ctgacgggga gggggcgctt gacgtaggac
     1321 ccggctgggc ctagcccgcc cgtgacatct cgcaaggagg cccgatgcca accctcgtcc
     1381 tgctccgcca cggccagagc cagtggaatc tcgagaaccg cttcaccggc tgggtggacg
     1441 tcgacctgac cgccgagggc gaggcccagg cccgcaaggg cggcgagctg atcaaggccg
     1501 ccggcatcga tctggacgag gcctatacct cggttcagac gcgcgcgatc cgaacctgca
     1561 atctggcgct ggacgctgct ggccaaagct tcgtgccggt gaccaaggac tggcgtctga
     1621 acgagcgcca ctatggcggg ctgacgggcc tgaacaaggc cgagaccgcc gtcctgcacg
     1681 gcgaagagca ggtcaccatc tggcgccggt cctacgacat tccgccgccg gaactggcgg
     1741 ccgggagcga gtacgacttc agcaaggacc gccgctacgc tggcaaggac ctgccgacca
     1801 ccgaaagtct ggccaccacg ctggttcggg tgctgcccta ttgggaagcc gagatagcgc
     1861 caaagctggc ggcgggcgag acgatcctgg tcgccgccca tggcaactcg ctgcgggcca
     1921 tcgtcaagca cctgttccag gtgccggacg ctgacatcgt ccacgtcgaa atccccactg
     1981 gaaacccgct ggtgatcgag ctggatggcg ccctgaagcc ggttgcggtc cgctacctgg
     2041 acgagagccg cgcccagacc ctgcccgcca tcgcctgagt gcgacggaag cgcttggaaa
     2101 cgctgcgtta atgccggggc gggcatggtc gcc
//
LOCUS       DAOMEO010000561         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5441_length_2132_cov_2.811748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000561 DAOMEO010000000
VERSION     DAOMEO010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5441_length_2132_cov_2.811748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PLH31_12250"
     CDS             <1..1979
                     /locus_tag="PLH31_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR90004.1"
                     /translation="LDHAQRLLERDPRLARQQAEAILEAVPRHAGTTVVLASALRLSG
                     EAEQALATIAPLAKGLPTLPEAQLELGLTLAELGRTAEAVAAFRRTVILDPRAGEAWR
                     GLAEGLDLLADKAGAETALAQQLRASTRDPALIEAAAALIDNRLGEAEQRLRDHLADH
                     PADVAALRMLAETGARLGRYEDAERLLVGCLEIAPGFAAARHNLATMLYRQNKSAEAL
                     AQIERLTDRDPRHPGYANLRAAILSRLGEYDTAIGIYERMLAEFPGQPKGWMSYGHAL
                     KTVGRQADCVAAYRKCLDLAPGLGEAWWSLANLKTVKFDAADRAAMAAGLEQPDLTDD
                     DRLHLHYALGKALEDARLYADSFQHYAAGAAIRRQQVDYDPDENHADVARSRAVFTPA
                     LLAATAGQGCPAPDPIFVVGLPRSGSTLIEQILASHSQVEGTMELPDLIVMARRLGGK
                     ANRRADSTYPEMLAELSPEALRELGEEYLERTRVQRKTDRPFFIDKMPNNFAHTGLIH
                     LILPKAKIIDARRHPLGCCFSGFKQHFARGQAFSYDLTDIGRYYADYVALMDHFDTVL
                     PGRVHRVIYEAMIADPEGQIRALLDHCGLPFEPACLSFHENDRAVRTASSEQVRQPIF
                     KDAVEHWQNFESDLEPLKQALGQCLATYPYAPRI"
BASE COUNT          349 a          738 c          699 g          346 t
ORIGIN      
        1 ccctcgacca tgcccagcgc ctgctggagc gcgatccacg gctggctcgt cagcaggccg
       61 aagcgatcct ggaggcggtc ccgcgtcatg ccggcacgac cgtggtgctg gcgtccgccc
      121 tgcggctgag cggcgaggcc gagcaggcgc tggcgaccat cgcgccgctg gccaagggct
      181 tgccgaccct gcccgaagcc cagctggagc tgggcctgac cctggccgag ctgggccgca
      241 ccgccgaggc cgtcgcggcc ttccggcgaa cagtgatcct cgatccccgc gcgggcgaag
      301 cctggcgcgg cctggccgag gggctggacc tgctggcgga caaggccggc gccgagacgg
      361 ccctggccca gcaactgcgc gcttcgaccc gcgatccggc cctgatcgag gccgccgccg
      421 ccctgatcga caaccgcctg ggcgaggccg agcagcggct tcgcgaccac ctcgccgatc
      481 accccgccga cgtcgccgcc ctgcgcatgc tggcagagac cggcgcgcgg ctgggtcggt
      541 acgaagacgc cgaacggctg ctggtcggct gtctggagat cgcgccgggc tttgcggccg
      601 cgcggcacaa tctggcgacc atgctctatc gccagaacaa gtcggccgag gctcttgccc
      661 agatcgagcg cctgaccgat cgcgatccgc gccatcctgg ctatgccaat ctgcgcgccg
      721 ccatcctgtc gcggctgggt gagtacgaca ccgccattgg catctatgag cggatgctgg
      781 ccgagttccc cggtcagccc aagggctgga tgagctatgg ccacgcgctg aagaccgttg
      841 ggcgtcaggc cgactgcgtc gccgcctatc gcaagtgtct ggacctggcg ccgggcctgg
      901 gcgaggcttg gtggagtctg gccaacctca agaccgtcaa gttcgacgcc gccgaccgcg
      961 ccgctatggc cgccgggctg gagcagccgg acctgacgga cgatgatcgc ctgcacctgc
     1021 actatgccct cggcaaggcg ctggaggacg cgcggctcta tgccgactcc ttccagcact
     1081 atgccgccgg cgcggcgatc cggcgccagc aggtggacta cgatccggac gaaaaccacg
     1141 ccgatgtggc ccgctcacgg gcggtgttta ccccggccct gttggccgcg acagcggggc
     1201 agggctgccc cgcgcctgat ccgatctttg tggttggctt gccgcggtcg ggctcgaccc
     1261 tgatcgagca gatcctggcc agccacagtc aggtcgaggg cacgatggag ctgcctgacc
     1321 tgatcgtcat ggcccgccgg ctgggcggca aggccaaccg gcgcgctgac tcgacctatc
     1381 ccgagatgct ggccgagctg tcgccggaag ccttgcgcga gctgggcgag gagtatctcg
     1441 agcgcacacg cgtgcagcgg aaaaccgatc gcccgttctt catcgacaag atgcccaaca
     1501 acttcgcgca cacgggtctg atccacctga tcctgcccaa ggcgaaaatc atcgatgcac
     1561 ggcggcatcc gctgggctgc tgcttttcgg gcttcaagca gcacttcgcc cggggtcagg
     1621 ccttcagcta cgacctgacc gacatcggcc gctactatgc ggactatgtg gcgctgatgg
     1681 atcatttcga caccgtgctg ccgggccgcg tccaccgggt gatctacgag gcgatgatcg
     1741 ccgatcccga aggccagatc cgcgccctgc tggaccattg cggcctgccg ttcgagcccg
     1801 cctgcctgag cttccacgag aacgaccggg ccgtgcgcac cgccagttcc gaacaggttc
     1861 gccagccgat cttcaaggac gccgtcgagc attggcagaa tttcgaatcg gacctggagc
     1921 ccctcaaaca ggcattgggc caatgtctgg cgacttatcc gtacgcgccc agaatttgaa
     1981 cacctcgggc agggtctgcc atttctgcaa cactcggaag attcgggttt tttagacgct
     2041 ttcagccttg ccgagcgctt gggcggagcg gaacatcgtt ctcttaaaat ggtacggggg
     2101 aacgaccatg acgtcgcgta agaacttcaa gc
//
LOCUS       DAOMEO010000562         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5448_length_2131_cov_2.685453, whole genome shotgun sequence.
ACCESSION   DAOMEO010000562 DAOMEO010000000
VERSION     DAOMEO010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5448_length_2131_cov_2.685453"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..572)
                     /locus_tag="PLH31_12255"
     CDS             complement(123..572)
                     /locus_tag="PLH31_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02444 family protein"
                     /protein_id="HQR90005.1"
                     /translation="MTLWDWALQVYARQDVADACLSLQDAHGQNVPYLLWAAWAAGEG
                     RVADAKAAARLMKQWDAEVGAPLRGVRRALKAPFPAVADTAREPFRQALKDVKLQGER
                     VLLESLEALSGPAGPPLDVLEGLLAAAAASGDPPRRSALERLATALA"
     gene            complement(577..1194)
                     /locus_tag="PLH31_12260"
     CDS             complement(577..1194)
                     /locus_tag="PLH31_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQR90006.1"
                     /translation="MPDPIKTADRPRLIVGISGASGVIYGIRALDACRDLGVESHLVL
                     SKSAALTLSQETDLSVSEVNAKADIVHRAADVGAAIASGSFRTLGMVIAPCSVRTMSE
                     IASGVTSSLLTRAADVVLKERRTLALMVRETPLHLGHLRTLTKLSEMGAVIAPPLPAF
                     YAKPDSIAEMVDQSVGRVLDLFDLNWRPVKRWGEDIAPIAGKSES"
     gene            1325..1498
                     /locus_tag="PLH31_12265"
     CDS             1325..1498
                     /locus_tag="PLH31_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HQR90007.1"
                     /translation="MAIESRIRELGSRHENLDRSIQEETKRPASDATRLRELKRQKLR
                     LKEEIEGLRSQLH"
     assembly_gap    1513..1522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1551..>2131)
                     /locus_tag="PLH31_12270"
     CDS             complement(1551..>2131)
                     /locus_tag="PLH31_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HQR90008.1"
                     /translation="RGADPIGNGQLTREELDRAQANDAYRMKAQVSRHAELLKPQKKA
                     PRYTPVSRRQDRPDAIAWFLRHHPEVTDAQISKLLGTTKSTIDQVRQRSHWNAANIKP
                     VDPVTLGLVGQLELDALVKKAAEKKAKDDLKKGILPEDPTLRPASETGVVETPEEDEE
                     ADYRKSRAEPKAEDVFGKSTYVADDEDEDDED"
BASE COUNT          344 a          692 c          726 g          359 t
ORIGIN      
        1 cgtccgacgg ctcgtcgtcg ttcgtcgacg gttcatcggt catggatggt tcccctcggc
       61 cggcccgcca acccgataga tagccccgat agatagcaaa ggctgctggc gcgtaaaggg
      121 cctcacgcca aagccgtcgc caggcgttcc agggccgagc ggcgcggcgg atcgccagac
      181 gccgcggcgg cggccagcag gccttccaga acgtccagcg gcggaccggc ggggccgctc
      241 agggcctcca ggctctccag cagcacgcgc tcgccctgca gcttcacgtc cttgagcgcc
      301 tggcggaagg gttcgcgcgc cgtgtcggcg acggccggga agggggcctt cagcgcccgg
      361 cgcacgccgc gcaggggcgc gcccacctcg gcgtcccatt gcttcatcag ccgggcggcg
      421 gccttggcgt cggcgacgcg gccctcgccg gcggcccagg cggcccacag caggtagggc
      481 acgttctggc cgtgggcgtc ctgcaggctc agacaggcgt ccgcgacatc ctgtcgcgca
      541 tagacttgca aagcccagtc ccagagcgtc atggcgttaa ctttcactct ttccggcgat
      601 cggcgcgatg tcctcgcccc agcgtttgac gggtcgccag ttcaggtcga acaggtccag
      661 cacccggccc accgactggt cgaccatttc ggcgatcgag tccggcttgg cgtagaaggc
      721 cggcaggggc ggggcgatga ccgcgcccat ctccgacagc ttggtcaggg tgcgcagatg
      781 gcccaggtgc agcggcgtct cgcgcaccat cagggccagg gtccgacgct ccttcagcac
      841 cacatcggcg gcgcgggtca gcagggagga ggttacgccg ctggcgattt cgctcatcgt
      901 gcgcaccgag cagggggcga tgaccatgcc cagggtgcgg aacgagcccg aggcgatggc
      961 ggcgcccaca tcggcggcgc gatgcacgat atcggccttg gcgttgacct cggaaaccga
     1021 aagatcggtc tcctgcgaca gggtcagggc ggccgatttg gagagcacca gatggctctc
     1081 gacgcccagg tcacggcagg cgtccagggc gcgaatgcca tagataacgc ccgaagcgcc
     1141 tgagattccg acgatcagtc tggggcgatc ggcggtcttg atggggtccg gcatggtgaa
     1201 ttttcgtccg acacgggagt aaggcgcgct acgtcaagcg gggccgcgtg caaccatatg
     1261 tgatctgaca gccgagcgca gcgggtgttc actccttaag gtcaacagca aggagccgac
     1321 agccatggcg atcgaatccc gtatccgcga acttgggtcc cgtcacgaga accttgatcg
     1381 ctcgatccag gaagagacca agcgtcccgc cagcgatgcg acccgacttc gagagctcaa
     1441 gcgacaaaag ctcaggctga aggaagagat cgagggcctc agatcccagc tgcactgaag
     1501 atcatataga aannnnnnnn nnggcctttt tcatgggtcg cgagacggga tcagtcttcg
     1561 tcgtcctcgt cctcatcgtc ggccacatag gtcgacttgc cgaacacgtc ctcggccttg
     1621 ggctcggccc gcgacttgcg gtaatcggcc tcttcgtcct cctcgggcgt ctcgacgacg
     1681 ccggtctccg acgcgggacg cagggtggga tcttccggca agatgccctt cttcaggtcg
     1741 tccttggcct tcttctcggc cgccttcttg accagggcgt ccagctccag ctggcccacc
     1801 aggcccaggg tcaccggatc gaccggcttg atgttggccg cgttccagtg cgagcgctga
     1861 cgcacctggt cgatggtcga cttggtggtg cccagcagct tggagatctg ggcgtcggtc
     1921 acctcggggt ggtggcgcag gaaccaggcg atggcgtcgg ggcgatcctg acggcgcgac
     1981 acgggcgtgt agcgcggggc cttcttctga ggcttcagca gttcagcgtg gcggctgacc
     2041 tgggccttca tgcgataggc gtcattggcc tgggcgcggt ccagctcttc gcgggtcagc
     2101 tggccattgc cgatcgggtc ggcgccgcgg a
//
LOCUS       DAOMEO010000563         2130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5450_length_2130_cov_1.654458, whole genome shotgun sequence.
ACCESSION   DAOMEO010000563 DAOMEO010000000
VERSION     DAOMEO010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_2130_cov_1.654458"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>357)
                     /locus_tag="PLH31_12275"
     CDS             complement(<1..>357)
                     /locus_tag="PLH31_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR90009.1"
                     /translation="DIAYSVNPASPMIGLARDGGITASAVTPVLSGVGGGSHEHADDG
                     VIEEMTAGASGSGDPPLFGGQAAFVRLQAGDPDLVEASKLAVTVSLGEAGARAAGGSR
                     GATIVLIKSALEDARAF"
     assembly_gap    358..367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<368..687)
                     /locus_tag="PLH31_12280"
     CDS             complement(<368..687)
                     /locus_tag="PLH31_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR90010.1"
                     /translation="MIRSILLTGAACAALAAPAAAQTIAITNARLEPISGPAIASGTI
                     VLRDGKIAALGAGVTAPAGATVIDAKGGVVTPGLIAPSSNLTASEISGVRETRDDGTG
                     TALSA"
     gene            complement(687..2130)
                     /locus_tag="PLH31_12285"
                     /pseudo
     CDS             complement(687..2130)
                     /locus_tag="PLH31_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079376.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
BASE COUNT          317 a          690 c          741 g          372 t
ORIGIN      
        1 gaaggcgcgg gcatcttcca gcgcagactt gatcagcacg atagtcgcgc cgcgcgaacc
       61 gccagccgcg cgggccccgg cttcgcccag cgacaccgtg accgccagct tcgaggcctc
      121 gaccagatca ggatcgccag cctgcaggcg aacgaaggcg gcctggccgc cgaacagggg
      181 cggatcgccc gagcccgaag cgccggccgt catctcctcg atcacgccgt catcggcatg
      241 ttcgtgcgat ccgccgccga cgcccgaaag cacgggcgtc acggccgagg cggtgatgcc
      301 gccgtcgcgg gccagaccga tcatcggcga ggccggattg acgctgtagg caatgtcnnn
      361 nnnnnnngca gacagggccg tgccggtgcc gtcgtcgcgc gtttcgcgca cgccgctgat
      421 ttccgacgcg gtcaggttcg aggacggcgc gatcaggccg ggggtgacta ccccgccctt
      481 ggcgtcgatc accgtcgcgc cagcaggcgc cgtgacccct gcgccgagcg cagcgatctt
      541 gccgtcccgc aggacaatcg tgcccgaggc gatggccggg ccgctgatcg gctccagccg
      601 ggcgttggtg atggcgatgg tctgggccgc tgccggagcg gcgagggcgg cgcaggcggc
      661 gcccgtcagg agaatggacc ggatcatcag ttgaacgctc cctggccggt ttggccaagc
      721 tcgaagtcgg acttgggctg gtagcgcgtg tcatgacggt cataggtcag ggcgccgtcg
      781 acatagacct tctcggcctg ggcgtagacg ctgaacggat cacaaggtcg gcggccttgc
      841 cgggctcgat ggcgccggtc ttgtcgccga tccccatggc cttggcggga ttggcggtga
      901 tccacttgat cgcctcggcc cgggtgatgc cgtagcccag cttgttcccg gccgtcatgg
      961 cgatggcggc ttcctggttc aggcgctggg tcatgatcgg atcgtcggag tggatgacga
     1021 cgcaggcgcc ggccttccag acgagggcgg ggttggcgtc gatgccgtcc aggctctcca
     1081 tcttgaagcc cgtccacgag gcccaggtgg ccgaacagat gtcttccttg gccagcaggg
     1141 cgcccacctt gtaggactcg atggcgtggt ggaacatcgt gatcttgtag ccaaactcct
     1201 tggcgatatc gatcatcacg gccatttcgt cgccgcgata gcagtggttc tggaccagga
     1261 tctcgccctt cagcacgccg gcgagggttt ccagcttgag gtcgcgcttg ggcgcatcag
     1321 ccttttcgcc cttgtccttc ttggcgcgat agtcgtccca cttgcgggcg tagtcggctg
     1381 catcgatcca ggcggcgcgg aagccggcga cattgcccat cgccgtcgcc ggtgagcgac
     1441 ccttgccgcc atagacgcgc ttgcggttct cgccgcaggc catcttcagg ccatacggcg
     1501 cgccggggaa cttcatgccc tgcatggtgg tcgacggcac attcttcagt gtcaccgatc
     1561 gaccgccgaa caggttggcc gagccaggca ggatcatcag ggttgtgacg ccgccggcgc
     1621 gagcccggtt gaagccgggg tcctgcggcc agaccgagtg ctcggaccag acctgcgacg
     1681 tattgggatc agtggcctcg ttgccgtccg acttggcttg cacgccgggc gagggataga
     1741 cgcccagatg gctgtgggcg tcgatgatgc cgggcgtcag ccacttgccc ttggcgtcga
     1801 tgaccgcgac atcggccggg atcggggtct cgggaccgcc aaccgaggcg atcttgccgc
     1861 ccgaaacgaa gacgacgccg ttgtcgattt gctgacccgt cgccgtcagg attgtcgcgc
     1921 cgacgatggc cgtagcgcgg gagggcaggg gcgcataggt cgaggggaag ccggaggagg
     1981 tggcgccgtc cagccccttg ggcagtggct tgttctcgac cttttcggcc ggcttggcct
     2041 ttgtttcgcc gggcttgccg cctgctccgc tcgtggtcgc gcaggccgaa agacccaacg
     2101 caagcaagct tacggccgca gccgtatgac
//
LOCUS       DAOMEO010000564         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5458_length_2126_cov_2.155480, whole genome shotgun sequence.
ACCESSION   DAOMEO010000564 DAOMEO010000000
VERSION     DAOMEO010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_2126_cov_2.155480"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLH31_12290"
     CDS             <1..690
                     /locus_tag="PLH31_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQR90011.1"
                     /translation="EHQPAKVMAMEGHFESHPDGAPLILFGIPDREAATVHGAVEIPK
                     LSSLILKHDPNAPLAGLDTIARADWPPVEIVFWSFRVMVGLGMAMLLLGLWGLYARLR
                     KRLYDAPWLHRFALVMGPMGFVAVLAGWITTEVGRQPWTVYGLLRTADSAAPLAAPAV
                     ATSLAAFAVVYFTVFGAGVFYILRLMSHAPHRDEPGVAETPIRTAGITPAPAIDPDRP
                     IPTGEETDHDR"
     gene            680..1681
                     /gene="cydB"
                     /locus_tag="PLH31_12295"
     CDS             680..1681
                     /gene="cydB"
                     /locus_tag="PLH31_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQR90012.1"
                     /translation="MTVDLTLVWAGLLAFAVFAYIVMDGFDLGVGILFPTLKAGDQRD
                     KAMNSIAPVWDGNETWLILGGGGLFAAFPLAYAVLMPAVYTPIIAMLLGLVFRGVAFE
                     YRWRTVRAKPVWDIAFFGGSLLAAFSQGVTLGAILQGVEVSGRAYGGGWWDWLSPFSL
                     LTGAAVVVGYALLGATWLNMKTEGALQTHARAQAWKLGPATLLMIGAVSLATPFLDGE
                     YARRWFDWPGVLLTAQVPLLVLIVAAAFFWTMRQKREVWPFLLSLGLFAVTFAGLGVS
                     VFPYAVPDEITLWQAAAPASSQLFMLVGALILIPIILAYTAYAYWVFRGKVGDDGYH"
     gene            1874..2119
                     /locus_tag="PLH31_12300"
     CDS             1874..2119
                     /locus_tag="PLH31_12300"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR90013.1"
                     /translation="MSFARSGFAQLIASGWGRLLRIIVGLGLIAWGYTMRDASTGVAL
                     MVVGLAPLLAGVFDLCLISPLLGGPLSGRRIRSARPL"
BASE COUNT          262 a          762 c          679 g          423 t
ORIGIN      
        1 gagcatcagc ccgccaaggt catggcgatg gaaggccatt tcgaaagcca cccggacggg
       61 gcgccgctga tcctgttcgg cattcctgac cgcgaggcgg ccaccgtcca cggcgcggtc
      121 gagatcccca agctgtcgtc cctgatcctg aagcacgatc cgaacgcgcc cctggccggt
      181 ctcgacacca tcgcccgcgc cgactggccg ccggtggaga tcgtcttctg gtccttccgc
      241 gtcatggtcg gcctgggcat ggccatgctg ctgctgggac tctggggtct ttacgcccgg
      301 ctgcgcaaac gcctctatga cgcgccctgg ctgcaccgct tcgccctggt catggggccg
      361 atgggcttcg tcgccgtgct ggccggctgg atcaccaccg aggtcggccg tcagccctgg
      421 accgtctacg gactgttgcg caccgccgac agcgccgcgc ccttggcggc gcccgccgtc
      481 gcaacctccc tggccgcctt cgctgtggtc tatttcactg tcttcggcgc cggggtcttc
      541 tacatcctgc gtctgatgag ccatgcgccg caccgcgacg agccgggcgt ggcggaaacc
      601 ccgatccgca cggcgggcat cacccccgcc cccgccatcg acccggaccg ccccatcccg
      661 accggcgagg agaccgatca tgaccgttga tctgaccctg gtctgggccg gccttctggc
      721 cttcgccgtc ttcgcctata tcgtcatgga tgggttcgac ctgggagtcg gcatcctgtt
      781 cccgacgctg aaggctggcg atcagcgcga caaggcgatg aactcgatcg ccccggtgtg
      841 ggacggcaat gagacctggc tgatcctggg cggcggcggc ctgttcgccg cctttcccct
      901 cgcctacgcc gtgttgatgc cggcggtcta tacgccgatc atcgccatgc tgctcggcct
      961 ggtcttccgg ggcgtcgcct ttgaataccg ctggcgcacg gtgcgggcca agcccgtctg
     1021 ggacatcgcc ttcttcggcg gatccctgct ggccgccttc tcccagggcg tcaccctggg
     1081 ggccatcctg cagggggtcg aggtgtcggg ccgcgcctat ggcggcggct ggtgggactg
     1141 gctcagcccc ttcagcctgc tgaccggcgc cgccgtcgtc gtcggctacg cccttctggg
     1201 ggccacctgg ctgaacatga agacggaagg cgcgctccag acccacgccc gcgcccaagc
     1261 ctggaaactg ggtccggcga ccctgctgat gatcggcgcc gtcagcctgg ccacgccttt
     1321 cctagacggc gaatacgccc ggcgctggtt cgactggccc ggcgtgcttc tgacggccca
     1381 ggtgccgttg ctcgttctga tcgtcgcggc cgccttcttc tggaccatgc gtcagaagcg
     1441 tgaggtctgg cccttcctgc tgtctctggg cctgttcgcc gtcaccttcg cggggctggg
     1501 cgtcagcgtc ttcccctacg ccgtgcccga cgagatcacc ctctggcagg ccgccgcccc
     1561 cgcctccagc cagctgttca tgctggtcgg cgccctgatc ctgatcccga tcatcctggc
     1621 ttacaccgcc tatgcctact gggtgttccg gggcaaggtc ggcgacgacg gttaccactg
     1681 atgcgtcgcg actccagaaa cgggctccgg cagggccttt ggttcgtcgc cctctgggtg
     1741 ctgggcgtgg gtgtcctggg cgtgatcgcc tatctcctcc gcgccgtcct cacgccggtg
     1801 ctcacataac aatggaacat cgttttctcg ccgacccatc gtaagggccg gctccagcca
     1861 gaaagagaag ctcatgtctt tcgcgcgttc gggtttcgcc cagttgatcg ccagcggctg
     1921 ggggcggctg ctgcggatca tcgtcggtct cggtctgatc gcctggggct atacgatgag
     1981 ggacgccagc acgggcgtgg cgctgatggt cgtgggtctt gcccccctgt tggcgggcgt
     2041 cttcgatctt tgtctgatca gccccttgct cggcgggccg ctgagcggtc gtcgcatccg
     2101 ttcagcccgc ccgctctgaa cccttc
//
LOCUS       DAOMEO010000565         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5461_length_2125_cov_2.923671, whole genome shotgun sequence.
ACCESSION   DAOMEO010000565 DAOMEO010000000
VERSION     DAOMEO010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_2125_cov_2.923671"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1523)
                     /locus_tag="PLH31_12305"
     CDS             complement(18..1523)
                     /locus_tag="PLH31_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR90014.1"
                     /translation="MTETVSPPDIPTPPAARRFPWPRGLSARLLLFTAFVVTLAGLMV
                     LPPTLAAYEEQWLLDRVRAGELASTIAESDPELRVSDSVAGQLFDQAGAVTVAVQVDG
                     ARRLVLPPKVPMQTPYLVDLRRQNPGSWLAAPFFTLTSPPGSMVRVMAEPRFRKAEFV
                     EVVLPDAPLKAQLVAYFWRLAAITVFVAGLAGFFVYLFLNIFLVRPMQRITRAMEQFR
                     ADPADPEARIVVSKRRDEIGRAELELDRMQTDLLTALASKARLAALGEAVAKINHDLR
                     NMLTSAQMASDRLAALGDPKVAQALPRLERALDRAITLATDVMTYGKSREPAPVVRPT
                     PLRAALDSAAEDSGLVAAGGVALETVIDPREQVLADPDQLHRILTNLLRNAREAIEGA
                     PDRGGKGRVFVELHRGDGLSILRLSDDGPGVPQRAQANLFQPFVGSVRRGGTGLGLAI
                     ARELAQGHGGDLVLVETGPGGSVFDLTLSGVPEPLTEPAALAAAPGDSAAP"
     gene            complement(1533..>2125)
                     /locus_tag="PLH31_12310"
     CDS             complement(1533..>2125)
                     /locus_tag="PLH31_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQR90015.1"
                     /translation="FAGEHPVLSVATYIALYVVTVSISLPGALILSLSGGFLFGPWGG
                     GAAAVTGATGGSTVVFLVCRTAFGDFLRRWPTRLLLAIEAGFRKDAFSYLLTLRLIPA
                     FPLLLVNVGAGLVGIPVRTFLLASFLGMVPSSFVYAGIGAGLGHVFARGGPVSVESLL
                     SPRIYLPIIGMGMLAFLPPLWRHWRHRRVAPAPDVK"
BASE COUNT          366 a          714 c          801 g          244 t
ORIGIN      
        1 gaccgggttt gacgccgtca gggcgcggcg ctgtcgcccg gcgccgcggc cagggcggcg
       61 ggctcggtca gcggctcggg aacgcccgac agcgtcagat cgaacaccga gccgccgggg
      121 ccggtctcga ccagcaccag gtcaccgccg tggccctggg ccagttcgcg ggcgatggcc
      181 aggccaaggc cggtgccgcc gcggcggacc gagccgacaa aaggctggaa gaggttggcc
      241 tgggcgcgtt gcggcacgcc ggggccgtca tccgacaggc gcaggatgga caggccgtcg
      301 ccgcgatgca gctcgacaaa cacccggccc ttgccgccgc gatcgggggc gccctcgatg
      361 gcctcgcggg cgttgcgcag caggttggtg aggatgcgat gcaactggtc gggatcggcc
      421 agcacctgct cgcgcgggtc gatcacggtc tccagggcga cgccgccggc ggccaccagg
      481 cccgagtcct cggcggcgct gtccagcgcg gcgcgcaggg gcgtggggcg caccaccggg
      541 gccggctccc tggacttgcc ataggtcatg acgtcggtgg ccagggtgat cgcgcggtcc
      601 agggcccgct ccaggcgcgg cagggcctgg gccaccttgg gatcgcccag ggcggccagc
      661 cggtccgacg ccatctgggc gctggtcagc atgttgcgca ggtcgtgatt gatcttggcc
      721 accgcttcgc ccagggccgc cagccgggcc ttggaggcca gggcggtcag caggtcggtc
      781 tgcatgcggt ccagttccag ctcggcgcgg ccgatctcgt cgcggcgctt ggacaccacg
      841 atgcgggctt ccggatcggc ggggtcggcg cggaactgtt ccatggcgcg ggtgatgcgc
      901 tgcatggggc gcaccaggaa gatgttgagg aacagataga cgaagaagcc cgccaggccg
      961 gcgacgaaca ccgtgatggc cgccaggcgc cagaaatagg cgaccagctg ggccttcagc
     1021 ggggcgtcgg ggaggaccac ctcgacaaat tcggccttgc ggaagcgcgg ctcggccatc
     1081 acccgcacca tgctgccggg cgggctggtc agggtgaaga acggcgcggc cagccatgag
     1141 ccggggttct ggcggcgcag atcgaccaga tagggcgtct gcatcgggac cttgggcggc
     1201 aagacgaggc gccgggcgcc gtcgacctgc accgcgacgg tgaccgcacc ggcctggtcg
     1261 aacagctggc cggcgacgga atcgctgacc cgcagctcgg gatcgctctc ggcgatggtc
     1321 gaggccagct cgccggcccg cacgcggtcc agcagccact gctcctcata ggccgccagg
     1381 gtgggcggca gcaccatcag gccagccagg gtgacgacga aggcggtgaa cagcagcagg
     1441 cgcgccgaca ggccgcgcgg ccacggaaac cggcgcgcgg cgggcggggt cgggatatcc
     1501 ggcggcgaga ccgtctcggt catggaactg ggttacttca cgtcgggcgc tggagcaacg
     1561 cgccggtgtc gccaatggcg ccacagcggc ggcagaaagg ccagcatgcc catgccgatg
     1621 atcggcagat agatcctggg cgacagcagg ctttccaccg agaccggtcc gcccctggcg
     1681 aagacatgcc ccaggccggc gccgatgccg gcatagacga acgagctggg aaccatgccc
     1741 aggaacgagg ccaggaggaa ggtgcgcacc ggtatgccca ccagacccgc gcccacattg
     1801 accagcagca acggaaaggc cgggatcagc cgcagggtca gcagatagga gaacgcgtcc
     1861 ttgcgaaatc ccgcctcgat cgccagcagc agccgggtgg gccagcgacg caggaagtcg
     1921 ccaaaggcgg tgcggcagac caggaacacc acggtagagc cgccggtcgc cccggtgacc
     1981 gccgccgccc cgccgcccca gggcccaaac aggaagccgc cagacagcga caggatcagg
     2041 gcgccgggca gggagatcga aaccgtgacc acatagaggg cgatataggt cgcgacgctg
     2101 agcaccgggt gctcgcccgc aaagg
//
LOCUS       DAOMEO010000566         2123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5468_length_2123_cov_2.058027, whole genome shotgun sequence.
ACCESSION   DAOMEO010000566 DAOMEO010000000
VERSION     DAOMEO010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_2123_cov_2.058027"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLH31_12315"
     CDS             complement(<1..281)
                     /locus_tag="PLH31_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90016.1"
                     /translation="MLYRPFGISGMTVSAITLRLSDQSRLRAGEWRALVFAAIENGVN
                     SFQIDGSSPALLQGAAEAFASVERRLLFLTWRLRDEAGQITRDIVDRMMA"
     gene            complement(284..517)
                     /locus_tag="PLH31_12320"
     CDS             complement(284..517)
                     /locus_tag="PLH31_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HQR90017.1"
                     /translation="MPMSPADLEGRLQAAFPDAEIVLTDLAGDGDHYKARIVSPAFRG
                     LSRVKQHQMVNRALADVLGGTLHALALETAAPD"
     gene            666..1598
                     /locus_tag="PLH31_12325"
     CDS             666..1598
                     /locus_tag="PLH31_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQR90018.1"
                     /translation="MIQVLVPSLRLFALILAFGVSTSACAATPGARALEDVRILSDDA
                     MQGRAPGTPGSALARAHILKRFGEIGLSPIGDSFERPFSYARRDGARVDAINLVARIP
                     GTQKSGKVMVVSAHYDHLGVRGGEIFNGADDNASGVAGLLAVAEAFKAPPRHDVILAV
                     VDAEEGGLRGAHAFVADPPVPLASIALNINFDMLSKNVRNELYASGASAWPALRPILD
                     KVAATAPVTLRQGHDTDADGKQNNWTTQSDHYAFAAKGVPWVYFGVEDHPEYHKSTDD
                     FATIPQDFFKRSIATVVAASLALERDLDTLQPSR"
     gene            1708..>2123
                     /locus_tag="PLH31_12330"
     CDS             1708..>2123
                     /locus_tag="PLH31_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR90019.1"
                     /translation="MLRDTIIRTAKVTALVVCVAMAMDYIVNILIMKHPAAYSPWATL
                     VISTIIAGPLAYSLNSQKHGLLKLQAELSGTLQAKQLAIEEVERRRAEAEDALVRLRE
                     SDRLYRLLADNLTDSINLFSRSGKRLYMSPSIEKLTG"
BASE COUNT          372 a          697 c          690 g          364 t
ORIGIN      
        1 gccatcatcc tgtcgacgat gtcgcgcgtg atctgtccgg cctcgtcgcg caggcgccag
       61 gtcaggaaca gcaggcggcg ctcgaccgag gcgaaggctt ccgccgcgcc ctgcagcagg
      121 gcgggcgagc tgccgtcgat ctggaagctg ttgacaccgt tctcgatggc cgcaaacacc
      181 agcgcgcgcc actcgccggc gcgcaggcgc gactggtctg acaggcgaag ggtgatcgcc
      241 gaaaccgtca tgcctgaaat gccgaacggc cggtacagca tggctagtcg ggcgccgcgg
      301 tttcgagcgc cagggcgtgc agagtcccgc ccagcacatc ggccagggcg cgattgacca
      361 tctggtgctg tttgacccgg ctcagaccgc ggaaagccgg cgagacgatg cgagccttgt
      421 agtgatcgcc gtcgccggcc agatcggtga ggacgatctc ggcgtcggga aaggcggctt
      481 gcaggcggcc ttcgaggtcg gcgggagaca tgggcacggg aggaaagctc caatcctgaa
      541 agggctgtat ggcccgcaag tcttaagaac gggcgtatgc ggtcggggca gggcgtcttt
      601 tggtcacagg catggttaag tggatttaac tccctctgga agccgcctgc gggcatacgg
      661 tcgacatgat tcaggttctt gttccaagcc ttcgcctttt cgccctgatt ttggcttttg
      721 gcgtatcgac ctcggcctgc gccgcgacgc cgggcgcccg ggcgctggag gatgtccgca
      781 tcctgtcgga cgatgcgatg cagggccgcg cgccgggaac gccgggctca gccctggccc
      841 gcgcccacat cctcaagcgc ttcggcgaaa tcggcctctc gccgatcggc gacagcttcg
      901 agcggccgtt cagctacgcc cggcgcgacg gcgccagggt cgacgccatc aatctagtgg
      961 cccgcattcc cggaacccag aagagcggca aggtcatggt cgtctcggcc cattacgacc
     1021 atctgggcgt tcggggcggc gaaatcttca atggcgccga cgacaatgcc tcgggtgtgg
     1081 ccggcctgct ggccgtcgcc gaggccttca aggccccgcc ccggcacgac gtgatcctgg
     1141 cggttgtcga cgccgaggag ggcggcctgc gcggggctca cgcctttgtc gccgatccgc
     1201 ccgtgccgct cgcaagcatc gccctgaaca tcaatttcga catgctgagc aagaacgtca
     1261 ggaacgagct ctatgccagc ggcgcaagcg cctggccggc gctgaggccg atcctcgaca
     1321 aggtggcggc caccgcgccc gtcacgcttc ggcaggggca cgacaccgac gccgacggca
     1381 agcagaacaa ctggaccacc cagtccgatc actacgcctt cgcggccaag ggcgtgccct
     1441 gggtgtattt cggcgttgag gaccaccccg agtatcacaa gagcacggac gacttcgcca
     1501 cgatcccgca ggatttcttc aagcgctcga tcgccaccgt cgtggccgcg agcctggcgt
     1561 tggagcggga cctggatacg ctccagcctt cccgctgatc ccgggccagc gcggccaatt
     1621 gccactcccg ttggcatcgc agatcatctg atcttaagct cgcctccggc atgctgggtt
     1681 ggaaaaggat tcgccgcgat cgaccggatg ctgcgcgaca cgatcatcag aacggccaag
     1741 gtcaccgcgc tggtcgtctg cgtcgccatg gcgatggact atatcgtcaa catcctgatc
     1801 atgaaacatc cggcggccta ttccccctgg gccaccttgg tgatctccac catcatcgcc
     1861 ggtccgctgg cctactccct caatagccag aagcatggcc tgctgaagtt gcaggctgag
     1921 ctgtccggca ccctgcaggc caagcagttg gccatcgagg aagtcgagcg ccgccgggcc
     1981 gaggccgagg atgcgctggt gcgattgcgc gaaagcgatc gtctgtatcg gctgctggcc
     2041 gacaatctga ccgacagcat caatctgttt tcccgctcgg gaaagcggct ctacatgtcg
     2101 ccatcgatcg agaagctgac ggg
//
LOCUS       DAOMEO010000567         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5477_length_2121_cov_2.475799, whole genome shotgun sequence.
ACCESSION   DAOMEO010000567 DAOMEO010000000
VERSION     DAOMEO010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5477_length_2121_cov_2.475799"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLH31_12335"
     CDS             complement(<1..1340)
                     /locus_tag="PLH31_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HQR90020.1"
                     /translation="MFKLHFRELLRFMIDGAAAERSFKIVRNGETEPALASWTADLAG
                     VQKHIADALNARNVAFLLGAGCSSLRSGDDELGISTMMPLAQQFCDTTHQSISAAKQA
                     AEDASLEEPSAEDDEREPGDLDDDTSAEVVLAPGAPADPGPAPAPAPAPAWALTADEL
                     SFLEGYGIALEEENARNLERLMEVLHSARFVLRRSSRSPDWASADRLTLIIDKVQLFI
                     WEKCTNGAFARPAGEKSDTKVRDLYEQFYRKLVLRDRSLPRPWVFTTNYDLFNETAMD
                     RLAIPYSNGFSGVVERRFNPATFRYALAEQLDISSRKWSAVDAYVYLCKLHGSISWTE
                     DDHGLFPIRERVPPEGGTKMMIFPTPAKQNSSLGSPYSDLFREFQGRIVQEQSVLITL
                     GYAFGDEHLNNIIYQALTIPTFRLIIFFDPTAKDEIAKLRALGDPRITIIGGDGS"
     gene            complement(1613..>2121)
                     /locus_tag="PLH31_12340"
     CDS             complement(1613..>2121)
                     /locus_tag="PLH31_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR90021.1"
                     /translation="AHNAAFDRRFLERFCETFTTKAWACSMSQVDWAEEGHEGTKLAY
                     LAAGAGFFYDRHRATNDCLAAVALLGAPLPRSGRFALAQLLERARQPTWRVWAENSPF
                     DLKDVLKARGYRWNGEGSGSPKAWFIDVTDGAKEAELAFLKTEIYRGEIDLLTRRIDA
                     YDRFSDRC"
BASE COUNT          387 a          677 c          655 g          402 t
ORIGIN      
        1 gacccgtcgc cgccgatgat cgtgatccgg gggtcgccca gcgctcgcaa cttggcgatc
       61 tcatcctttg cggtcggatc gaagaagatg atcaaccgaa acgtcgggat ggtcagggct
      121 tggtagatga tgttgttcag gtgctcgtcc ccaaaggcgt agccgagggt gatgaggaca
      181 ctctgctcct ggacgatccg gccctggaac tcccggaaaa ggtcggagta aggcgagccg
      241 aggctggagt tctgtttggc gggggtgggg aagatcatca tcttcgtgcc cccctcgggc
      301 ggcacccgct cgcggatcgg gaagaggccg tggtcgtcct cggtccagct gatcgagccg
      361 tggagcttgc aaaggtagac gtatgcatcg acggccgacc acttgcggct ggagatatcc
      421 agttgttcgg ccagggcgta acggaacgtg gcggggttga accggcgttc gaccacgccg
      481 ctgaagccgt tcgaatatgg gatcgccagg cggtccatgg ccgtctcgtt gaacaggtcg
      541 tagttggtgg tgaagaccca gggccgggga agactgcggt cgcgcagcac cagctttcgg
      601 taaaactgtt cgtagaggtc gcgcaccttg gtgtcgctct tctcgccggc cgggcgggcg
      661 aacgcgccgt tggtgcattt ttcccagatg aacagctgaa ccttgtcgat gatgagcgtc
      721 aggcgatccg ctgaagccca atcgggtgag cggctgcttc gacgcagcac aaaccgcgcg
      781 ctgtgcagca cctccatcaa ccgctcgaga ttgcgggcgt tctcttcctc gagggcgatg
      841 ccgtagcctt caaggaagct caactcatcg gccgtcagag cccaggccgg ggccggggcc
      901 ggggcggggg cgggcccggg atccgccggc gccccaggcg ccaggacaac ctcggctgac
      961 gtgtcgtcgt ccaaatcccc gggctctcgc tcgtcgtcct ccgccgaagg ctcctccaga
     1021 ctggcgtcct ctgccgcttg cttggcggcc gagatgctct gatgggtggt gtcgcagaat
     1081 tgctgggcaa ggggcatcat cgtgctgatg ccaagctcgt catcgccaga tcgaagagac
     1141 gaacaaccgg ccccaagcag aaacgcgacg ttgcgcgcgt tcagggcatc ggcgatgtgc
     1201 ttttggacac ccgccagatc ggcggtccaa ctcgcgagcg ccggctcagt ttcgccattg
     1261 cgaacaatct tgaaagaccg ctctgcggct gcaccatcga tcatgaatcg cagaagctcc
     1321 ctaaagtgca gcttgaacaa ctagcggttt tatcactgtc ccgcgataac ctgatcgacg
     1381 gcgcgcgaac taatcgcttc cctgcgacgt agttgggtgc cttccatgca agcctgcgtt
     1441 tggaactaga atggtgcgct ctcccccggc accacaaaat ccccgtgtca tctttggtga
     1501 gcgcggctgt tgtctcggcg cttacgagat atccaactcc aagacgtagg cggggatctg
     1561 gacccttgac ttgccctatt gctcgccccg accccggttt cgcctgacag attcaacaac
     1621 ggtctgaaaa cctatcgtag gcgtcgatcc gacgggtgag caggtcgatc tcaccccggt
     1681 agatttccgt tttcaggaac gccagctcgg cttccttagc cccgtcagta acgtcgatga
     1741 accaggcctt cggcgaacca cttccctccc cgttccaccg atagccccgc gccttcagca
     1801 catccttcag gtcgaacggc gagttctccg cccaaacccg ccacgtcggc tgacgcgccc
     1861 gctccagcaa ctgcgcgagc gcgaacctcc cactccgcgg cagcggcgcc ccaagcagcg
     1921 cgacggcggc caggcagtcg tttgtcgccc gatgccggtc ataaaagaac cccgcgccgg
     1981 ccgcgaggta ggccagcttc gtcccctcgt ggccttcctc ggcccagtcc acctggctca
     2041 tcgaacaggc ccaggccttc gtggtgaagg tctcgcagaa ccgctccaga aatcgacgat
     2101 cgaaggccgc attgtgggcg a
//
LOCUS       DAOMEO010000568         2120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5480_length_2120_cov_1.933172, whole genome shotgun sequence.
ACCESSION   DAOMEO010000568 DAOMEO010000000
VERSION     DAOMEO010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5480_length_2120_cov_1.933172"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLH31_12345"
     CDS             complement(<1..570)
                     /locus_tag="PLH31_12345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HQR90022.1"
                     /translation="MSAKLITLPGLLTPSAAYKPFRYPWAHEFWKKQQQVHWMPEEIP
                     LGEDLKDWAVKLNDKERNLLTQIFRFFTQADVEVADNYMERYGRVFKPTEVKMMLSSF
                     ANMETIHIAAYALLLETIGMPESEFGAFMEYEAMRDKHDFMQKFGVETNADICRTLAM
                     FGGFTEGLQLFASFAMLMNFPRFNKMKGMG"
     gene            705..1379
                     /locus_tag="PLH31_12350"
     CDS             705..1379
                     /locus_tag="PLH31_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR90023.1"
                     /translation="MVNPAYTIYGALGSGSVPVEATLTLLGQPYAVIEAPTWEGESEQ
                     AKVAVVNPLKQIPALVTPAGETITESAAILIWLADNHPQAGLAPALDSPERAQFLRWM
                     TFIPASIYSLFWVRDDPSRLVGDDAEMQAKVKAATAERILECWRMMESQVSPGRYLLG
                     DQMTVLDLYVAVVSRWGPRRVRFYEVAPRMAEVVRRVDADPRLAAFWAERFPFFDGWE
                     RLGQQG"
     gene            1478..1825
                     /locus_tag="PLH31_12355"
     CDS             1478..1825
                     /locus_tag="PLH31_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90024.1"
                     /translation="MSSNPPVPSSRLTIQEYTRVWWAIIWRNSLFGFVAGTLLGGVAG
                     LILSTFGYGHLGAPVGAFMGALGNIVISFFVIKHVLAKRFAGFEIVVLRSEDNVRHPS
                     LSDSPSAPDAATP"
BASE COUNT          363 a          640 c          696 g          421 t
ORIGIN      
        1 gcccatgccc ttcatcttgt tgaagcgcgg gaagttcatc agcatcgcga acgacgcgaa
       61 cagctgcagg ccctcggtga agccgccaaa catcgccagg gtgcggcaga tgtcggcatt
      121 ggtctcgacg ccgaacttct gcatgaagtc gtgcttgtcg cgcatggcct cgtattccat
      181 gaacgcgccg aactcgcttt ccggcatgcc gatggtctcg agcagcaggg cgtaggccgc
      241 gatatggatg gtctccatat tggcgaacga cgacagcatc atcttcactt cggtgggttt
      301 gaacacccgg ccgtagcgct ccatgtagtt gtcggccacc tcgacgtcgg cctgggtgaa
      361 gaagcggaag atctgcgtca gcaggttccg ttccttgtca ttgagcttga cggcccagtc
      421 cttgagatcc tcgcccagag ggatttcctc gggcatccag tggacctgct gctgcttttt
      481 ccagaactcg tgagcccacg gatagcggaa gggcttgtag gctgccgaag gcgtcaggag
      541 accaggaagg gtgataagct tggcggacat cggcagaggc tccagggacg gacggacacg
      601 aatcggacgg acgacttcag gctctcttcg taccgcagcc aacaggttca tctagtggtc
      661 agttggtcgc agcacacaac atgttgtggg tagattacta atcaatggtt aatccagcat
      721 acaccatcta cggcgcgctt ggctcgggtt ccgtacccgt cgaggccact ttgaccctgc
      781 tgggccagcc ctatgcggtg atagaggcgc cgacctggga aggcgagtcc gagcaggcca
      841 aggtggccgt ggtcaacccg ttgaaacaga tcccggccct ggtcaccccg gccggcgaga
      901 cgatcaccga gagcgccgcg atcctgatct ggctggccga caaccatccg caggcgggcc
      961 tggccccggc gttggattcg ccggagcgcg cccagttcct gcgctggatg acctttatcc
     1021 ccgcctcgat ctattcgctg ttctgggtgc gcgacgatcc ctcgcgcctg gtcggcgacg
     1081 acgccgaaat gcaggcgaag gtcaaggccg ccaccgccga gcggattttg gaatgctggc
     1141 gaatgatgga gagtcaggtt tcgccggggc gctacctgct gggcgaccag atgaccgtgc
     1201 tcgacctcta tgtcgccgtc gtcagccgct gggggcctcg gcgcgtgcgg ttctacgagg
     1261 tcgcgccacg gatggcggag gtggtccggc gggtggacgc cgatcctagg ctggcggcgt
     1321 tctgggcgga gcggtttccg ttttttgacg ggtgggagcg gttggggcag caggggtgat
     1381 aagctcggag cgaaagtcga gaggctggcg cgacgtcggc tgttgccgcc cccaactgca
     1441 ggcttgcggc ctcagcaggt ctgcttaagg tagagctatg agctcgaacc cgcccgtccc
     1501 atcgtcgcgc cttaccatcc aggaatatac cagagtctgg tgggcaatta tctggcgcaa
     1561 ctcgctgttc ggctttgtcg cgggcaccct gttgggcggg gtggcgggct tgattctcag
     1621 cacgtttggc tacgggcatc tgggggctcc tgtaggcgcg tttatgggtg ctcttggcaa
     1681 catcgtgatc agcttcttcg tcataaagca cgtcttggcg aagcgatttg cggggtttga
     1741 aatcgtcgta cttcggagcg aagataacgt ccgccaccca tcactgtcgg acagcccatc
     1801 cgccccagat gcggccacgc cttaaaccct tggtggatcg gagctctggc gtctgaccgt
     1861 gtcaaggacc gcctccggcg gccgcgtcgc ggcgacgcgg agcgtcgtcc ttgagacggt
     1921 cagacgccag agcaggctcg ccagcaaggt tctaaggcgc gggctcgccg acacgggcgg
     1981 tcattttgag gagccccacc ccttctcccc ttgcgggaga aggtggcccc ggcgaaggcc
     2041 ggggtcggat gaggggtcgc gccggcccat ccggacaggt ccttcacccc ctcatccgtc
     2101 ggctgcgccg acaccttctc
//
LOCUS       DAOMEO010000569         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5490_length_2117_cov_2.591659, whole genome shotgun sequence.
ACCESSION   DAOMEO010000569 DAOMEO010000000
VERSION     DAOMEO010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_2117_cov_2.591659"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>258
                     /locus_tag="PLH31_12360"
     CDS             22..>258
                     /locus_tag="PLH31_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR90025.1"
                     /translation="MSAAAADFSECSMRGAKLVGANLKNASLRGAVIDNVDVRGANLE
                     SADFTDAVVTRVDLTQAKMMGAVMDRCLCDPGPEA"
     assembly_gap    259..268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <269..809
                     /locus_tag="PLH31_12365"
     CDS             <269..809
                     /locus_tag="PLH31_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR90026.1"
                     /translation="EASAKAAAIFVKLAAHREWVTSGGKAGQPAVLDGEDLRPIADMA
                     GAFLTAVSARNACLAGMDLRRISLQGALLEGADLRGAILREADLRGANLTAANLRQAD
                     LRRTMLGALPFKVDREKPTVLAEACLRYADLREADLGDTDLADADLHGAKRAAARPAV
                     VDDMGVDDADSDETFVLDM"
     gene            complement(810..>1559)
                     /locus_tag="PLH31_12370"
     CDS             complement(810..>1559)
                     /locus_tag="PLH31_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQR90027.1"
                     /translation="IDRFDEIVELDTPLVEYAVRVAGDIERRIIEQIAALIPNGATVQ
                     AGIGRLGDALWAGLSGKEGLALHSGMICDPVADLVRHGLFRRVTTGSLLGSRAFYARA
                     ADLPGVEMAPALETHDVARLAKIPGFVAVNSAIEVDLLGQVNAEWVDGAAISGPGGLP
                     DFIGGARRSSGGLPIIALGSTAKGGAVSRIVPRLQVPATLSAAEVGIVVTEHGAVDLR
                     PLEGEARAMALIELAAPDHRAALREARRRGA"
     assembly_gap    1560..1569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1570..2014)
                     /locus_tag="PLH31_12375"
     CDS             complement(<1570..2014)
                     /locus_tag="PLH31_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90028.1"
                     /translation="MSIMPPDTRVFVSASAAEPSAAAPLSRLLEPLRSATFEGGFIPG
                     VNSFDWSSLGPQARMAGPFMSTAWQAIAAAGRFEHLPVTYHGFARRVAASRYAAGLFM
                     VTPPDQTGSCSFGPTTDFAPTALDRCGLKIGVINPSLTRLPGSPAV"
BASE COUNT          355 a          702 c          729 g          311 t
ORIGIN      
        1 gacaagtccc ggctggacga tgtgtcggcg gcggcggcgg acttttccga atgttccatg
       61 cgcggcgcaa agctcgttgg cgccaacctc aagaatgcca gcctgcgcgg cgctgtgatc
      121 gacaatgtcg acgtgcgggg ggccaatctc gagagcgccg atttcaccga tgcggtggtg
      181 acgcgcgtcg acctgaccca ggccaagatg atgggagccg tcatggatcg ttgcctgtgc
      241 gatcccgggc cggaagccnn nnnnnnnngg aagccagcgc caaagcggcg gcgatctttg
      301 tgaagctggc ggcgcaccgt gaatgggtca ccagtggcgg caaggcgggc cagcccgctg
      361 tgctggacgg agaagacctg cggcctatcg ccgacatggc cggcgccttc ctgaccgctg
      421 tcagcgcccg gaacgcctgc ctggcgggca tggatcttcg gcggatttct ctgcagggtg
      481 cgctgctgga gggcgccgac ctgcgtggcg ccatcctgcg cgaagccgac ctgcgcggcg
      541 ccaatctgac cgccgccaat ctgaggcagg ctgatctgcg ccgtacgatg ctcggcgcgc
      601 tacccttcaa ggtcgatcgc gagaaaccca cggtcctggc ggaggcctgc ctgcgctacg
      661 ctgaccttcg tgaggctgat ctgggcgaca cagacctggc cgacgcagat ctgcacggcg
      721 ccaaacgcgc ggcggcgcgc ccagctgtgg tcgatgacat gggggtcgac gacgcggata
      781 gcgacgagac gttcgttctg gacatgtagt caggcgccgc gtcgccgcgc ctcgcgcaag
      841 gccgcgcgat ggtccggggc cgcgagctcg atcagggcca tggcgcgggc ctcgccctcc
      901 agcggccgca gatcgacagc gccatgctcg gtcacgacga tcccaacctc ggccgccgat
      961 agcgtggccg ggacctgcag gcggggaacg atgcggctga ccgcgccgcc cttcgcagtc
     1021 gaacccaggg cgatgatggg taggccgcca gacgagcgcc gcgcgccgcc gatgaagtcc
     1081 ggcaagccac ccggtcccga gatcgccgcc ccgtccaccc actcggcgtt cacctggccc
     1141 agcagatcga cctcgatggc tgagttgacc gccacaaagc ctgggatctt ggcgaggcgg
     1201 gcgacatcgt gggtttcaag ggcgggcgcc atctccacgc ctggcaggtc ggcggccctt
     1261 gcgtaaaagg cccgagagcc gagcaggctg ccggtcgtca cgcgtcggaa caggccatgg
     1321 cgaacaaggt cggcgaccgg atcacagatc atgccgctgt gcagggcaag accctccttg
     1381 ccgctcagcc cggcccacag ggcgtcgccc agccgaccga tcccggcttg aacggtcgct
     1441 ccgttcggta tcagcgcggc gatctgttcg atgatccgcc gctcgatgtc tcccgcgaca
     1501 cgcacggcgt attcgaccaa gggcgtgtcg agctcgacga tctcgtcgaa cctgtcaatn
     1561 nnnnnnnnna gacggccgga gatccgggca accgggtcaa ggacgggttg atgacgccaa
     1621 tcttcagccc acatcggtcc agcgccgtcg gggcgaaatc tgtggtcggg ccgaacgaac
     1681 acgatcccgt ctgatcgggc ggtgtgacca tgaacagccc cgccgcatat cgactggcgg
     1741 ccacccgccg agcaaagccg tgataggtca cgggcaggtg ttcgaaccgc cccgccgccg
     1801 cgatggcctg ccaggccgtt gacatgaagg ggccggccat acgcgcctgg gggccaaggc
     1861 tgctccagtc gaagctgttg acgccgggaa tgaagccgcc ttcgaaggtc gccgatcgca
     1921 ggggttccag cagccgcgac aacggcgcgg cggcggaggg ctcggcggcc gaggcgctca
     1981 cgaaaacccg ggtgtctggc ggcatgatgc tcatcggctt ggcaggtgat gcgccgcagc
     2041 ataggcgaaa gcgacgcggg tgtggcgcct tccgccaggt ctggcggcct tgcccctaaa
     2101 gcccgatcac ccagtcg
//
LOCUS       DAOMEO010000570         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5511_length_2109_cov_2.537488, whole genome shotgun sequence.
ACCESSION   DAOMEO010000570 DAOMEO010000000
VERSION     DAOMEO010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5511_length_2109_cov_2.537488"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1388
                     /locus_tag="PLH31_12380"
     CDS             222..1388
                     /locus_tag="PLH31_12380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR90029.1"
                     /translation="MHIVDQIGRETTSFPNSLPALTSVRFALALGVVLFHYQLQWPWN
                     AMASTGVFDRAGLGVDAFFILSGFVLTHAYRQALEDGQLNYRRFLAARFARVYPAHIA
                     VLGFVLVMVGAGTLIGAEFDRRLYNPLGLLTTVLLVHAWLPETVVAEWNGPSWSLSAE
                     WFAYLAFPAFAWIGLVLARRPWILLALSALVFVALDLAYQAVFGDLVVHAQLNMGILR
                     IIPEFLYGIGLYRLGQRVQLTRRQAIGAAWLSAIILLLLMHFKADERVVVAAAGPLVL
                     SLALLSKSAADRAVARPSMLAAGEASYALYLVHMPILIGWKGVNSALTQRPSAYVLAW
                     WEVACLLVLTLVAAFAVHYLIERPARTWIRRGADRLWPATARKATAPRSRPPGP"
     gene            1497..1775
                     /locus_tag="PLH31_12385"
     CDS             1497..1775
                     /locus_tag="PLH31_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HQR90030.1"
                     /translation="MGHTVREKSKLLGRVRRIRGQVEAIERALEAETECAAVLQLIAS
                     VRGAMNGLMAEVMEDHIRMHVVDPASDPDPERARGAEELIDVVRTYLK"
     gene            1790..>2109
                     /locus_tag="PLH31_12390"
     CDS             1790..>2109
                     /locus_tag="PLH31_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90031.1"
                     /translation="MADQSNHDRATLADQAEARTTAPAASDEKKHPHRGHEQHGHDHY
                     DHDDEDGHDHEHPFEWLEAFRIGLVAIAAALVWFRVWEPIPTVSVIGAVGLLIGGWPI
                     LKET"
BASE COUNT          350 a          666 c          651 g          442 t
ORIGIN      
        1 cgtctgaggt ccttcgactg gtcaccaaga tacagctggg gcgatctcgt ccttgtcact
       61 acgtttcggt gggatcttag ccctcgccga tccggatgat ttttggcgcg cgccgtttgg
      121 gtccgttact ggcgcgcgga cgaccagtgg aattgcgttt cagcaagacg atgagggctg
      181 acctgtcaac cgcgcgtata cgcggcaggg cgggggctag aatgcacata gttgatcaga
      241 ttgggcgtga aacaacatcc ttccccaaca gtctgcccgc gctgacctcg gtccgctttg
      301 cgctggctct gggggtcgtg ctcttccact accaactaca atggccttgg aacgccatgg
      361 cctcgacggg cgtcttcgat cgcgctgggc tcggcgtcga cgccttcttc atcttgtctg
      421 gcttcgtcct cacccatgcc tatcgtcagg cgctagaaga cgggcagctg aactatcggc
      481 gcttcttggc ggcgcgcttc gcacgtgttt acccggctca catcgccgtt ttggggttcg
      541 tgctggtgat ggtcggcgca ggaaccctga tcggggctga gttcgatcgg cggctctaca
      601 atcccctcgg cctgctcacg accgtcctgc tggtgcacgc atggttgccg gaaacggtcg
      661 tggcggaatg gaacggccct tcatggtcgc tttccgccga atggttcgcc tatctcgcct
      721 ttcccgcctt cgcctggatc ggcctcgtcc tggcccgtcg accctggatc ctgctcgccc
      781 tgtcggcgct ggtcttcgtg gcgctggatc tggcctatca agcggtgttt ggggacttgg
      841 tcgtccacgc ccagctgaac atgggcatac tgcggatcat acccgagttt ctctatggga
      901 taggtcttta ccggttggga cagcgggtgc agctcactcg gcgccaggcg atcggcgcgg
      961 cttggctgtc ggcaatcatt ctattgctgc tgatgcactt caaagcggac gagcgggtcg
     1021 tcgtggccgc ggccggaccc ttggtcctca gtctggccct tctgtccaaa tccgccgccg
     1081 atcgcgcggt cgcccggccg tcgatgctgg ccgcaggcga ggcctcttac gccctttacc
     1141 tggtgcatat gcccatcttg attggatgga agggcgtcaa ttctgctctg acccagcggc
     1201 cgagcgccta cgtgctggcg tggtgggagg tcgcctgcct gcttgtgctc acgctcgttg
     1261 ccgcgttcgc tgtgcactac cttatcgagc gacccgcgcg aacatggatc cggcgcggcg
     1321 ccgatcgttt atggccagcg actgcccgaa aggcgacggc gcctcgctca cgccctccag
     1381 gcccctagcg gtgcggtggc cgttggacgc ctaaaccaaa accagaattg cagggtaagg
     1441 cctttccaag tataccctac ccccatatat tacgtttccc ggaacgcagg tgatctatgg
     1501 gacatacggt tcgcgaaaaa tccaagctgc tcggtcgcgt gcggcgcatt cgcggccaag
     1561 tggaagcgat cgaacgcgct ctggaggccg agactgaatg cgcagccgtg ctgcagctga
     1621 tcgcctccgt tcgcggcgcc atgaacggcc taatggccga ggtgatggaa gatcacatcc
     1681 ggatgcacgt cgttgatccg gccagcgacc ccgaccctga acgggctagg ggcgcggaag
     1741 aactcatcga cgtcgtgcgc acctacctga aatgaccagg aatacgacca tggccgacca
     1801 atcgaatcat gatcgcgcca ccctggccga tcaggctgaa gcacgaacga ccgcgccagc
     1861 ggcgtcggat gagaagaagc acccgcatcg cggccacgaa cagcatggac acgaccatta
     1921 tgatcacgac gatgaggacg gccacgacca cgagcatccg ttcgagtggc tggaggcctt
     1981 tcgcatcggc ttggtggcaa tcgccgcagc cctcgtctgg ttccgggtct gggagcccat
     2041 ccccactgtc agcgtgatcg gcgccgtcgg cctcctgatc ggcggctggc cgatcttgaa
     2101 ggagacgtt
//
LOCUS       DAOMEO010000571         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5532_length_2105_cov_2.783902, whole genome shotgun sequence.
ACCESSION   DAOMEO010000571 DAOMEO010000000
VERSION     DAOMEO010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5532_length_2105_cov_2.783902"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLH31_12395"
     CDS             complement(<1..303)
                     /locus_tag="PLH31_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR90032.1"
                     /translation="MMSDPVLSIRAASKTFGSRRALDAVSLDVSRGEMIALIGPSGSG
                     KSTLLRSIDGLQTIDEGLKGEDGGVITAFGGPVQARGKVSDQVRKARVRIGFIAQQF"
     gene            541..>753
                     /locus_tag="PLH31_12400"
     CDS             541..>753
                     /locus_tag="PLH31_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HQR90033.1"
                     /translation="MHTYHEPLDLVRERSPERPVALVRPDAVSVAARWFQAEFKGDVF
                     YAVKANPSPWVIRELVDAGVRSFDVAS"
     assembly_gap    754..763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <764..1775
                     /locus_tag="PLH31_12405"
     CDS             <764..1775
                     /locus_tag="PLH31_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HQR90034.1"
                     /translation="AFMHPVKSRVAISAAYFDHGVRTFSFDTHEELAKILDATGNAKD
                     LNLIVRMGVQAEGAAYSLSGKFGVESHAAPALLLAARRATQDMMGVSFHVGSQCMRPT
                     AYQAAMAQASRALVRAGVLADVVDVGGGFPSIYPGMVPPDMSEYLAAIDRGFAEMMVH
                     ETTELWCEPGRALVAEASSLLVKVELKKGDALYLNDGSYGSLFDAAHMKWPFPVKLYR
                     KNGADGGEMVEDGLKPFRFYGPTCDSVDHMPGPFYLPESVDEGDYIEIGMLGAYGVAM
                     STRFNGFGENETVEVQDAPMASMYGLAKRSIPTHKPESEERKVVRFSRPKGAAGKRKR
                     RR"
     assembly_gap    1820..1829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1876..>2105
                     /locus_tag="PLH31_12410"
     CDS             1876..>2105
                     /locus_tag="PLH31_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515739.1"
                     /note="transforms a conserved lysine residue of initiation
                     factor 5A into deoxyhypusine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HQR90035.1"
                     /translation="MFQRRASKVRETEAMNAPVPNTPTPNSKAELLQNVVEHIDITSF
                     DARPIIDAMRKMSFSSRDTARAADIFNMALED"
BASE COUNT          374 a          686 c          659 g          366 t
ORIGIN      
        1 gaactgctgg gcgatgaagc cgatgcgcac ccgggccttg cggacctggt cgctgacctt
       61 gcccctggcc tgaaccgggc cgccaaaggc ggtgatcacg cccccatctt cgcccttgag
      121 accctcatca atggtctgaa gaccatcgat cgagcgcaga agcgtggatt tgcccgagcc
      181 cgaggggccg atgagcgcga tcatctcgcc gcgactgacg tcgagcgaaa cggcgtccag
      241 cgcgcgcctg gacccgaagg tcttggaggc ggcgcggatc gagagaactg ggtccgacat
      301 catggtcggg gcgaatcctt aacgaggttt cgggccaagg ggaatgcgtt caccgctttg
      361 gaggccgatc gcaatggatt tgtgacatcc ggtggatcaa tttgcctcgg ggccgggggc
      421 cgaaaggtct ttacatccag aggttatgcc cctatctcgc actcctccgg cggacccgaa
      481 gtcctcataa gcgctgctaa cgccggccta ggtttcactt cttgcagggg gttacgtgca
      541 ttgcacacgt accatgaacc cctggacctg gtccgtgagc ggtcaccgga acgtcccgtc
      601 gcacttgtgc gaccggacgc ggtgtccgta gcggcgcgct ggttccaggc agaatttaaa
      661 ggcgatgtct tttacgcggt gaaggccaac ccttcgccgt gggtgatccg tgagctggtt
      721 gacgccggcg tgcgatcgtt cgacgtggcg tctnnnnnnn nnnggccttc atgcacccgg
      781 tcaagagccg ggtggcgatc tcggccgcct atttcgatca cggcgtgcgg accttctcgt
      841 tcgacaccca cgaagagctg gccaagatcc tcgacgccac cggtaacgcc aaggacctca
      901 acctgatcgt ccggatgggc gtgcaggccg agggagcggc ctacagcctg tcgggcaagt
      961 tcggcgtcga gtcgcacgcg gccccggccc tgctgctggc cgcccgccgc gccacccagg
     1021 acatgatggg cgtgtccttc cacgtcggca gccagtgcat gcggcctacg gcataccaag
     1081 ccgccatggc ccaggcctcc cgcgccctgg tgcgggcggg cgtgctggcc gatgtcgtcg
     1141 acgtgggcgg cggattcccg tcgatctatc ccggtatggt cccgccggac atgtccgaat
     1201 atctggccgc catcgaccgc ggcttcgccg agatgatggt gcacgagacg accgagctgt
     1261 ggtgcgaacc cggccgggcc ctggtggccg aagcctcgtc gctgctggtc aaggtagagc
     1321 tgaagaaggg cgacgcgctc tatctgaacg acgggtccta tggctcgctg ttcgacgccg
     1381 cgcacatgaa gtggcccttc ccggtcaagc tgtaccgcaa gaacggcgcc gatggcggcg
     1441 agatggtcga agacggcctc aagcctttcc gcttctacgg ccccacctgc gacagcgtcg
     1501 accacatgcc cggcccgttc tacctgccgg aaagtgtcga cgaaggcgat tacatcgaga
     1561 tcggcatgct gggcgcctat ggcgtggcca tgtcgacccg gttcaacggc tttggcgaga
     1621 acgagaccgt cgaggtccag gacgcgccca tggcttcgat gtacggcctg gccaagcgct
     1681 cgatcccgac ccacaagccg gagtcggaag agcgcaaggt cgtccgcttc tcgcgtccca
     1741 agggcgcggc cggcaagcgc aagcgtcgtc gctagttcga caccgttcgc cactagatca
     1801 tttctgagtt aaaccgcgcn nnnnnnnnng gttcgcattt ccgctcccgc cggttgctcc
     1861 gtcccggtga agagcatgtt ccaacgacgg gcgtccaaag tcagggaaac cgaagcgatg
     1921 aacgctcccg ttccgaatac ccccacgccg aacagcaagg ccgagctgct ccagaacgtg
     1981 gtcgagcaca tcgacatcac gtccttcgac gcccgcccga tcatcgacgc catgcgcaag
     2041 atgtcgttca gcagccgcga caccgcccgc gccgccgaca tcttcaacat ggccctggaa
     2101 gacaa
//
LOCUS       DAOMEO010000572         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5534_length_2105_cov_2.463902, whole genome shotgun sequence.
ACCESSION   DAOMEO010000572 DAOMEO010000000
VERSION     DAOMEO010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5534_length_2105_cov_2.463902"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLH31_12415"
     CDS             complement(<1..322)
                     /locus_tag="PLH31_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR90036.1"
                     /translation="MRYLHTMVRVRDLDASLRFYCQGLGLTEMYRMENDKGRFTLVFL
                     AAPEDVELARERKAPLVELTYNWDPEDYDGGRNFGHLAYRVDDIYETCQRLMDMGVTI
                     NRPPR"
     gene            complement(385..>2105)
                     /locus_tag="PLH31_12420"
     CDS             complement(385..>2105)
                     /locus_tag="PLH31_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HQR90037.1"
                     /translation="WRVALALAFTLIGKALGVLAPLLLGAAVNRLAAGQNVVTTVSWT
                     FAGLATGWALVRFISAAAPQARDAVFTPVAQAAQNRAAVETFAHALSLSIDFHQSKRT
                     GSLSRVIDRGARSMDFLLRSLVFNLGPTAIELLMAAAVLAKAYDWRFAAIALLTVGVY
                     GYITFAISDWRIGHRRALNEADAEAAGRAVDSLINYETVKSFGGEDRAVGGYKTALDV
                     YGDAQVKATNSLTVLNTVQAAVMSLGLGAMAVLAGMEASAGRMGPGDVTAAVLILINL
                     YAPLNILGFAYREIRQSFIDMEAMLDLRRQSPDVADAPDAIDLPPRADQRGGAVVFEA
                     VSFRHGARSEGLKDVSFSIPPGTTVAIVGPSGAGKTTLVRLALRMIDPQAGRITLDGC
                     DLRDLRQASLRRAVALVPQDVALFNDTVLSNIGFARPEATEAEIWAAAEAAELGEFIR
                     NLPEGMATKVGERGLKLSGGERQRVGIARALLAQPRVLILDEATSALDSRTEAAIQET
                     LRKARAGRTTLVVAHRLSTIADADEIVVLRRGRIVERGPHAALLEAGGEYAALWRRQT
                     REKPAA"
BASE COUNT          357 a          732 c          704 g          312 t
ORIGIN      
        1 cgcgcggcgg gcggttgatg gtcacgccca tgtccatgag acgctggcag gtctcgtaga
       61 tgtcatcgac ccgataggcc aggtggccga aattgcggcc gccgtcatag tcctcgggat
      121 cccagttgta ggtcagctcg accagcggcg ccttgcgctc cctggccagc tcaacgtcct
      181 caggcgcagc taggaagacc agggtgaaac ggcccttgtc gttttccatg cggtacatct
      241 cggtcaggcc caggccctgg caatagaagc gcagcgaagc gtccagatcg cggacgcgaa
      301 ccatggtgtg cagatagcgc aaggcgggac tcccaaactg tgtgcgatgg atatagcggt
      361 tcggcgcgat ctcgcgaagg gccgtcaggc tgcgggcttc tcgcgcgtct ggcgtcgcca
      421 cagggccgca tactctccgc ccgcttccag cagggcggca tgggggccac gctcaacgat
      481 cctgccacgt cgaagcacca cgatctcgtc ggcgtcagcg atcgtcgaaa gccggtgcgc
      541 caccaccagc gtcgtgcgcc ctgccctggc cttgcgcagc gtctcctgga tcgccgcctc
      601 ggtgcggctg tccagggcgc tggtggcctc gtccaggatc agcacgcgcg gctgggccag
      661 aagcgcccgg gcgatgccca cacgctgacg ctcgccgccc gacagcttca gtccccgctc
      721 gccaaccttc gtggccatgc cttcgggaag attgcggatg aactcgccca gctcggcggc
      781 ctcggcggcg gcccagatct cggcctcggt ggcctcgggt ctggcgaagc cgatattgct
      841 gagcacggtg tcattgaaca gggccacgtc ctgcggcacc agggcgaccg cacgacgcag
      901 cgaggcctga cgcaggtccc gcaggtcgca gccgtccagg gtgatcctgc cggcctgggg
      961 gtcgatcatc cgcagcgcca gacgcaccag agtggtcttg cccgcccccg atggcccaac
     1021 gatggccacg gtggttccgg gcgggatcga gaaactgacg tccttcagcc cttccgagcg
     1081 tgcgccatgc cggaacgaca ccgcctcgaa caccacggcg ccgccgcgct ggtcggcgcg
     1141 gggcggcagg tcgatggcgt caggcgcatc ggccacatcg gggctctggc ggcgcaggtc
     1201 gagcatggcc tccatgtcga tgaaggactg gcggatctcg cgataggcaa agcccaggat
     1261 gttgagcggc gcatacaggt tgatcaggat cagcacggcc gccgtgacgt cgcccggccc
     1321 catgcgcccg gccgaagctt ccatgcccgc cagcaccgcc atggcgccca gacccagact
     1381 catcaccgcc gcctgcacgg tattgagcac ggtcagggaa ttggtcgcct tgacctgcgc
     1441 gtcgccatac acatccagcg cggtcttgta gccgcccacc gcgcggtcct cgccgccgaa
     1501 ggacttcacc gtttcgtagt tgatcagcga gtcgactgcc ctgcccgcgg cctcggcgtc
     1561 ggcctcgttc agcgcgcgac gatggccgat gcgccagtcc gagatggcaa aggtgatgta
     1621 gccgtagacg ccgaccgtca gcagagcgat ggcggcgaac cgccagtcat aggccttggc
     1681 cagcacggcg gccgccatca gcagctcgat cgccgtcgga cccaggttga agaccaggct
     1741 gcgcagcagg aaatccatcg accgcgcgcc gcgatcgatc acgcgcgaca gcgagcccgt
     1801 gcgcttggac tggtggaaat cgatcgacaa tgacagggcg tgggcgaaag tctcgaccgc
     1861 cgcccggttc tgggccgcct gggccacggg cgtgaagacc gcatcccgcg cctgcggcgc
     1921 cgccgccgag atgaagcgaa ccagggccca gccggtcgcc aggcccgcaa aggtccagct
     1981 gacggtagtg acgacgtttt gcccggccgc caaccggttg actgccgcgc ccagcagcag
     2041 gggcgccaga acgcccaggg ccttgccaat cagggtgaag gccagggcca gagcgacacg
     2101 ccagc
//
LOCUS       DAOMEO010000573         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5541_length_2101_cov_2.422776, whole genome shotgun sequence.
ACCESSION   DAOMEO010000573 DAOMEO010000000
VERSION     DAOMEO010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_2101_cov_2.422776"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLH31_12425"
     CDS             <1..563
                     /locus_tag="PLH31_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90038.1"
                     /translation="SGLGLAITRRLAEMLGGEVVFQSEPGLGSRFTLTIAAPPAAAPA
                     SIERAPADILAGLRILLVEDNSTNQLVASRILEQLGAIVHTADDGLSGVEAARNGGFD
                     LVLMDVQMPGIDGLEASRRIRALPGPAARTPIIALTANVMAHQRASYTAAGMDGVAAK
                     PISPSALIAEILRLAESGACSETAVA"
     gene            632..>1249
                     /locus_tag="PLH31_12430"
     CDS             632..>1249
                     /locus_tag="PLH31_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90039.1"
                     /translation="MTDQTTKEEGASMRTVVAASSAGTAFEWYDFFIFGSLAQVISKT
                     FFTGLPETAGYIAALALFGVGFAFRPLGALVFGKIGDQAGRKGAFLATVLLMGGATFA
                     IAFLPTYAQAGIISPILLIILRCVQGFALGGEYGGAAIYVAEHSPNNKRGWSTSWVQT
                     SAAFGLFGALLVIMATRAIVGVEAFDAWGWRIPFAVSIGLLGISVW"
     assembly_gap    1250..1332
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1333..>2101
                     /locus_tag="PLH31_12435"
     CDS             <1333..>2101
                     /locus_tag="PLH31_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90040.1"
                     /translation="LKLTESPAFAKMKEEGEASKAPYAEAFGQWKNMKLVILAFLSMM
                     CAQGAVWYTSFFYSQTFMEKFLKVDPVTINALMMTATAVSAIFYVVFGWLSDKVGRKP
                     VMLAGMTLALAFYFPGFHLLERAANPALAEASARSPVSVIADPKDCALQFDPVGKTAF
                     VSSCDIVKSALANAGVSYDNQKAPAGTPAVMKVGDVVIASPSAQGLSKEETRAVKAAT
                     EAKIKAALTQAGYPAKADPARMNKLGMFGVLFVFVIAA"
BASE COUNT          315 a          673 c          669 g          361 t
ORIGIN      
        1 gatccggcct tggcctggca attacccgcc gactggccga gatgctgggc ggtgaggtcg
       61 tgtttcagtc tgaaccgggc ctcggttcgc gcttcaccct gaccatcgcc gcgccacccg
      121 ccgcggctcc ggcgtcgatc gagcgagccc cggcagatat cctggcaggt ctgcggattc
      181 tgctggtcga ggacaattcc accaaccagc tggtcgcctc gcgaatcctg gagcagctgg
      241 gggcgatcgt tcacacggct gacgacggcc tgtctggcgt ggaagccgcg cgaaacggcg
      301 gctttgacct ggtgctgatg gacgtccaga tgcccggcat agacggtctt gaagcctccc
      361 gccggatccg ggcgcttccc ggcccggccg ccaggacgcc tatcatcgcc ctgaccgcca
      421 atgtcatggc gcaccagcgg gccagctata ctgccgcggg catggacggg gtcgccgcca
      481 agccgatttc gccgtcggcg ctgatcgccg aaatcctgcg cctggccgag agcggggcgt
      541 gttcagagac cgctgtcgcc tgagtttccc ccgtcgtcga accacgctaa agctggcgcg
      601 aagagcgccg aacggctcca gggggaacag aatgactgac cagacgacca aggaagaagg
      661 cgcgtcgatg cgcaccgtcg tggcggcctc gtccgccggg acggcgttcg agtggtacga
      721 cttcttcatc ttcggaagcc tggcccaggt catttccaag accttcttca ccggcctgcc
      781 ggaaaccgcg ggctatatcg cggcgctcgc cctgttcggc gtcggcttcg cgttcaggcc
      841 tttgggcgcg ctggtgtttg gcaagatcgg cgaccaggcc ggccgcaagg gcgcgttcct
      901 cgccacggtg ctgctgatgg gcggcgcgac cttcgccatc gccttcctgc cgacctatgc
      961 gcaggcgggc atcatctcgc cgatcctgct gatcatcctg cgctgcgtgc agggctttgc
     1021 cctgggcggc gagtatggcg gggcggcaat ctatgtcgcc gagcactcac ccaacaacaa
     1081 gcgcggctgg tcgacctcgt gggtccagac ctcggcggcc tttggcctgt tcggcgccct
     1141 gctggtgatc atggccaccc gcgcgatcgt tggcgtcgag gcgtttgacg cctggggctg
     1201 gcgcatcccg ttcgccgtct cgatcggcct gctgggcatc tcggtctggn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nncctgaagc tcaccgaaag ccccgccttc gccaagatga aggaagaggg
     1381 cgaggcctcg aaagccccct acgcggaagc cttcggccag tggaagaaca tgaagctggt
     1441 gatcctggcc ttcctgtcga tgatgtgcgc ccagggcgcg gtttggtaca ccagcttctt
     1501 ctacagccag accttcatgg agaagttcct gaaggtcgat ccggtcacca tcaacgccct
     1561 gatgatgacg gccacggcgg tcagcgccat cttctacgtc gtctttggct ggctgtcgga
     1621 caaggtgggc cgtaagccgg tgatgctggc cggcatgacg ctggccctgg ccttctactt
     1681 cccgggcttc cacctgctgg agcgcgccgc caacccggcc ctggccgaag cgtcagcccg
     1741 gtcgccggtg tcggtgatcg ccgatccgaa agactgcgcc ctgcagttcg acccggtcgg
     1801 caagacggcg ttcgtctcgt cctgcgacat cgtcaagagc gccctggcca atgccggcgt
     1861 ctcctatgac aaccagaagg cgccggccgg gaccccggcg gtgatgaagg tcggcgacgt
     1921 cgtgatcgcc tcgcccagcg ctcagggcct gtcgaaagag gagaccaggg cggtcaaggc
     1981 cgcgacggag gcgaagatca aggcggcgct gacgcaggcc ggctatcccg ccaaggccga
     2041 tccggcccgg atgaacaagc tgggcatgtt cggcgtgctg ttcgtgttcg tgatcgccgc
     2101 g
//
LOCUS       DAOMEO010000574         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5548_length_2100_cov_2.602934, whole genome shotgun sequence.
ACCESSION   DAOMEO010000574 DAOMEO010000000
VERSION     DAOMEO010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_2100_cov_2.602934"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..880)
                     /locus_tag="PLH31_12440"
     CDS             complement(44..880)
                     /locus_tag="PLH31_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQR90041.1"
                     /translation="MSAPDRRLTLLRDGLADQRLEGLVPAQRFVAATPMQASAPIASI
                     RKAPAPDAEQEDQLAFGERFDVLFEVEGFAFGQARRDGYVGYVTSDALSAPVLAPTHR
                     VSALRTYGFSEPDIKSAIIGLYSLNALVTVEAREGRFVRVARSGWFIEAHLAPIGRGE
                     DDYVAVAERFLGAPYQWGGRESLGLDCSALVQQALAACNRACPRDTDLQREFFPEVAE
                     GERRRGDLVFWKGHVAILLDPETILHANAHHMATAIEPLAEAVARIEASGVGSPLGYR
                     RV"
     gene            complement(877..>2100)
                     /locus_tag="PLH31_12445"
     CDS             complement(877..>2100)
                     /locus_tag="PLH31_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HQR90042.1"
                     /translation="GQVERVLMGLGSRASVDPGLFRGLAARLPAGDYRLARLPRGLDA
                     QEATLAFALGLYRFDRYRKRSAEAGPRLVAPKGVDVEAVTAISHACALARDMVNTPAN
                     DMGPLQIETIAREIAEKHGAQISVITGDDLLAQNYPAVHAVGRAAAPHRAPRMIEISW
                     GDPAHPLVALVGKGVVFDTGGLDIKPSAGMRIMKKDMGGAAHALALARMVMGAQLPVR
                     LVVLTPVVENAISGDAMRPGDVLQTRAGLTVEVGNTDAEGRLILADALTRAAEHKPVL
                     TLDLATLTGAARVAMGPFVIPFWTSDDRLAGQIEAASKATFDPLWRLPLWDGYRDAID
                     SDIADLKNDPDAWAQAGATTAALFLERFAPTEGAWAHFDVYAWNPKSRPGYPTGGEAQ
                     AIRALFALLKMRFPA"
BASE COUNT          325 a          748 c          744 g          283 t
ORIGIN      
        1 ccctccgggc cacctccccc agagggggag gagctgcgag gcgctaaacc cgccgatacc
       61 ccagcggtga gccaacgccc gacgcctcga tccgcgcgac agcctcggcc aggggctcaa
      121 tcgccgtggc catgtggtgg gcgttggcgt gcaggatcgt ctcggggtcc aacaggatcg
      181 ccacgtggcc cttccagaac acaagatcgc cgcgacgccg ctcaccctca gccacttcag
      241 ggaagaactc gcgctgaagg tcggtgtcgc gcgggcaggc gcggttacag gccgccagcg
      301 cctgctggac cagggccgag cagtccagcc ccagcgactc gcgcccgccc cactgatagg
      361 gcgcacccag gaagcgttcg gcgacggcga catagtcatc ctcgccccgc ccgatcggcg
      421 ccaggtgcgc ctcgatgaac cagcccgacc gggcgacgcg gacaaagcgg ccttcgcggg
      481 cctcgaccgt cacaagggca ttgaggctgt agaggccgat gatcgccgac ttgatgtccg
      541 gctcgctgaa cccataggtg cggagggccg acacgcggtg ggtcggggcc aggaccgggg
      601 ccgagagggc gtcgctggtc acatagccca catagccgtc gcgacgagcc tggccgaagg
      661 caaagccctc gacctcgaac agcacgtcga accgctcgcc aaaggccagc tggtcttcct
      721 gctcggcgtc tggagccggc gccttgcgga tcgaggcgat cggcgcgctg gcctgcatcg
      781 gcgtggcggc gacgaagcgc tgggccggaa ccaggccctc cagccgctga tcggccaggc
      841 cgtcacgcag aagggtcagg cggcgatcgg gggcgctcat gccggaaacc gcatcttgag
      901 cagggcaaac agcgcccgga tcgcctgggc ctcgccgccg gtcggatagc ccggccggct
      961 cttggggttc caggcataga cgtcgaaatg ggcccaggcg ccctcggttg gcgcgaacct
     1021 ctccaggaac agcgccgctg tggtcgcccc ggcctgggcc caggcgtcgg ggtcgttctt
     1081 caggtcggcg atgtcgctgt cgatcgcgtc gcgataaccg tcccacaggg gcagacgcca
     1141 caggggatcg aaggtggcct tcgaggcagc ctcgatctgg ccggccagcc ggtcatcgct
     1201 ggtccagaac gggatcacga acggccccat cgccacccgc gccgcgccgg tcagggtggc
     1261 caggtccagg gtcaggacgg gcttgtgctc ggccgcccgg gtcagggcgt cggccaggat
     1321 caggcggcct tcggcgtcag tattgccgac ctccaccgtc aggccggcgc gggtctgcag
     1381 cacgtcgcca ggccgcatgg cgtcgccgct gatggcgttc tcgaccaccg gcgtcagcac
     1441 caccagccgc accggcagct gggcgcccat caccatccgc gccagggcca gggcgtgggc
     1501 ggcgccgccc atgtccttct tcatgatccg catgccggcc gagggcttga tgtccaggcc
     1561 gccggtgtcg aacaccacgc ccttgcccac cagggcgacc aacggatggg cgggatcgcc
     1621 ccagctgatc tcgatcatgc gcggcgcgcg atggggcgcg gcggcgcggc ccacagcgtg
     1681 gacggcggga tagttctgcg ccagcaggtc atcgccggtg atcaccgaga tctgcgcccc
     1741 gtgcttctcg gcgatctcgc gggcgatggt ctcgatctgc agcgggccca tgtcgttggc
     1801 gggcgtgttg accatgtcgc gggccagggc gcaggcatgg ctgatggcgg tgaccgcctc
     1861 gacatccacg cccttgggcg ccaccagacg aggccccgcc tctgcggaac gcttgcggta
     1921 ccggtcgaag cgatagaggc ccagggcgaa ggccagggtc gcctcctggg cgtccaggcc
     1981 gcgcggcagg cgggccagac gatagtcgcc cgccggcagc ctggcggcga ggcctcggaa
     2041 caggcccgga tcgacgctgg cgcggcttcc cagccccatc agcacgcgct caacctggcc
//
LOCUS       DAOMEO010000575         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5556_length_2097_cov_2.019099, whole genome shotgun sequence.
ACCESSION   DAOMEO010000575 DAOMEO010000000
VERSION     DAOMEO010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_2097_cov_2.019099"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="PLH31_12450"
     CDS             <1..133
                     /locus_tag="PLH31_12450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR90043.1"
                     /translation="VESIRRFPDQRTFATMMENAGFRRVTISNFTGGVAAMHQGWAV"
     gene            137..1614
                     /gene="ubiB"
                     /locus_tag="PLH31_12455"
                     /pseudo
     CDS             137..1614
                     /gene="ubiB"
                     /locus_tag="PLH31_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
     gene            complement(1615..>2097)
                     /locus_tag="PLH31_12460"
     CDS             complement(1615..>2097)
                     /locus_tag="PLH31_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90044.1"
                     /translation="DGGASAVRAPKGGERAVANTTTEAPPPSETRRGDPRDAPVRLVS
                     GKPFWAANRTRTAEENAERAFERNGEAFGAVTVDDYVEAAHRFVANPPKGAETLKRAN
                     GDTLIYDPGGNIFAVVSREGAPRALFKPDDGAAYWDVQKARENRRSTVAQGRGAADSG
                     "
BASE COUNT          268 a          751 c          745 g          333 t
ORIGIN      
        1 ggtcgagagc atccggcgct ttcctgacca gcgcactttc gcgacgatga tggaaaatgc
       61 cggcttcagg cgggtgacca tcagcaactt cacgggcggc gtcgcggcga tgcaccaggg
      121 ctgggccgtc tagtccatgg ggcagatgtc cgcgttctgg cgcctggtcg gcgcgggctg
      181 ggtgctgatc cgggccgacg ccctgatccc gcgcgagctg gatccggtgc tgccgccgcc
      241 ggcgcggctg gcggcgcgga cgctgcggct gttgtccggc ccccgcgcta agctggggcg
      301 accgggccag cggctggccg gcgccctaga aggcctgggt cccgccgcga tcaagatggg
      361 ccagttcctg tcgacccggg ccgacatctt cggcacgccg ttcgccgagg acctgtcgcg
      421 cctgaaggat cgtctgccgc ccttcccgct cagccaggcc cgcgaggaga tcgaacgcaa
      481 tctgggccgg ccgatcgaga ccctgttttc gcagctggac gagcccgtcg ccgccgcctc
      541 cctggcccag gcgcatccag cctggctgct ggacggccgc aaggtggcgg tgaaggtcct
      601 gcgccccggc atcgagcgcc aggtcgccca ggacagcgcc gtgctgcgcc tggccgccga
      661 tctggccgaa cggctggttc ccgaggcccg tcgcctgaag ccgcgcgact ttgtcgaggt
      721 ggtgatccgg gcgctggatc tggaaatgga cctgcgcttc gcgaggtgat ggccaaggac
      781 gccttcatga ccgccccggc cgtggtctgg gacggcgtcg gcaagcgggt cctgaccctg
      841 gcctgggccg agggtcagcc gctgtcggac cccgaggcgc tggcgctgcc gggtctggac
      901 cgcaaggccc tggcgcagaa catcacccgg ggcttcctgg cccaggccct ggatcacggc
      961 ctgttccacg ccgacctgca tgagggcaac ctgtttgtcg ccgccccggc cagggtggtg
     1021 gccgtcgact acggcatcat cggacgcctg ggtcccggcg agcggaaata tctggccgag
     1081 atcctgtacg gcttcctgaa ccgcgactac ccgcgcgtgg cccgcatcca tttcgacgcc
     1141 ggctatgtgc ccgcccacca ggatgtggac gccttcgccc aggccctgcg cgccgtgggc
     1201 gaaccggtgt ttggcctgga cgcccgcgac atctcgatgg gccggctgct ggcccagctg
     1261 ttcgagatca ccgccctgtt cgacatggcc ctgcggcccg aactggtgct gttgcagaag
     1321 accatggtga cggtcgaggg cgtggcccgt cggatcgacc cggcccacga catctggtcc
     1381 gccgccgatc cggtggtgcg ccgctggatc acccgcgagc tgtcccccgc cgccaaggtg
     1441 cgcgacttcg ccgacgacgc cctgaaggcg ctgcgggcca tggcgcgcct ggccgaagcg
     1501 cccgcgacgg tagccgtggc ggccgcgccg aggccccggc cgctggcctg gttcgcggcg
     1561 ggcgcagggg tggccaccct ggcctttacg gccggggcgc tgctgtttgg ctgatcagcc
     1621 gctgtcggcc gcgcctcggc cctgggcgac cgtcgaccgg cggttctcgc gggccttttg
     1681 cacatcccaa taggctgcgc catcgtcagg cttgaacagg gcccgggggg cgccctcgcg
     1741 cgagaccacc gcaaagatgt tgccgcccgg atcatagatc agggtgtcgc cattggcgcg
     1801 cttgagggtc tcggcgccct tgggcggatt ggcgacaaac ctgtgcgccg cctcgacata
     1861 gtcatcgacc gtgacggcgc caaaggcctc gccattgcgc tcaaaggccc gctcggcgtt
     1921 ttcctcggcc gtgcgggtgc gattggccgc ccagaagggc ttgcccgaca ccagacggac
     1981 cggcgcatcg cgcggatcac cgcgtcgggt ttcagacggc ggcggcgcct ctgtcgtcgt
     2041 gttggccacg gcgcgctcgc cgcccttggg cgcgcgaacc gcagacgccc cgccatc
//
LOCUS       DAOMEO010000576         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5564_length_2094_cov_3.057872, whole genome shotgun sequence.
ACCESSION   DAOMEO010000576 DAOMEO010000000
VERSION     DAOMEO010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5564_length_2094_cov_3.057872"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..297)
                     /locus_tag="PLH31_12465"
     tRNA            complement(221..297)
                     /locus_tag="PLH31_12465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Arg,seq:ccg)
     gene            complement(368..1690)
                     /locus_tag="PLH31_12470"
     CDS             complement(368..1690)
                     /locus_tag="PLH31_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HQR90045.1"
                     /translation="MSFLERQFGLKAQGTSLRTEALAGFTTFLTMAYIVIVNPAILGQ
                     AGMPVAAVAAATCISAGIGCILMGLIANYPIALAPGMGLNAYFTYTVVIGMGVPWQTA
                     LGCVFLSGVAFLILTVAGIRQLIVGAIPRPLFSAIAAGIGLFIAFIGLKEAGIIVANP
                     ATTVALGDLTKPTAILALTGLLIIGALMAWRVKGAILIGILLVAALGWFMGLSKVAPG
                     GYSFAELSATAFQLDIPGALHLNGAMGMALAEIIFVFLFVDLFDNVGTLVAVTKKAGL
                     VEPDGTIPRLNRILTADSVATIAGSVAGTSTVVSYIESASGVQAGGRTGLTAVIVGLL
                     FLATLFFAPLVQAIPTAATAPALILVGAMMIGALAEVEWDDPGVAIPAFLTLVTIPLT
                     FSIANGLAFGITSYAALKLVRGQARPSDWLLYVLAALFIVRFIYMGAE"
     gene            complement(1755..>2094)
                     /locus_tag="PLH31_12475"
     CDS             complement(1755..>2094)
                     /locus_tag="PLH31_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR90046.1"
                     /translation="CDQIAIVNRGEVVACEPTATLLKRMDSRNVVVTPEEPVTAAPML
                     AGFETKIRAGGAFSVSYRTGQSSVEQVLAAVRASGMHIKDIATEDPDLEDVFVSLTYG
                     DPNAADPTKD"
BASE COUNT          388 a          657 c          713 g          336 t
ORIGIN      
        1 cttttggtgg gcggactcta attattcctg gaggagtttc agggggtcac gtcaatctaa
       61 aggctcaggc gatttttgcg cacgtgctga tgaaaggccg agggtccttc cacgccgtct
      121 aacgtgcagc tgataggtgc gccttttagg ataatttcta gggtgcgccg cgacgacgtt
      181 ctgatttaac cagtaaaatc agagtcttaa ttggcggtaa tggcgcaccc gacaggattc
      241 gaacctgtga ccttcgcctt cggagggcga cactctatcc agctgagcta cgggtgcatc
      301 cgccttgcgg ggaatggcct cttagccgaa aggccgggcc ggcgcaaacg ggcttattgg
      361 cctcccccta ttctgcaccc atgtaaatga agcggacgat gaacagggcg gccagcacgt
      421 aaagcagcca gtcgctgggc cgggcctggc cgcgcaccag cttcagcgcc gcatagctgg
      481 tgatgccgaa ggccagaccg ttggcgatcg agaaggtcag cggaatggtg accagggtca
      541 ggaaggccgg gatcgctacg ccgggatcat cccactccac ctcggccagg gcgccgatca
      601 tcatcgcgcc gaccaggatc agggccgggg ccgtggcggc cgtcgggatg gcctggacca
      661 ggggcgcgaa gaacagggtg gccaggaaca gcaggcccac gatcaccgcc gtcaggcccg
      721 tacggccgcc ggcctgcacg ccggaggcgc tctcgatata gctgaccacg gtgctggtgc
      781 cggccaccga gccggcgatg gtggccaccg agtcggcggt caggatgcgg ttgaggcgcg
      841 ggatcgtgcc gtcgggctcg accaggccgg ccttcttggt cacggccacc agggtgccga
      901 cattgtcgaa caggtcgacg aacaggaaga cgaagatgat ctcggccagg gccatgccca
      961 tggccccgtt caggtgcagg gcgcccggga tatccagctg gaaggcggtg gccgaaagct
     1021 cggcgaacga atagccgccg ggcgccacct ttgacaggcc catgaaccag cccagcgccg
     1081 ccaccagcag gatgccgatc aggatcgcgc ccttcacccg ccaggccatc agggcgccga
     1141 tgatcagcag gccggtcagg gccaggatgg ccgtcggctt ggtcaggtcg cccagggcca
     1201 cggtggtggc cggattggcg acgatgatgc cggcctcctt caggccgatg aaggcgatga
     1261 acaggccgat gcccgccgcg atcgccgaga acagcggccg ggggattgcc ccgacaatca
     1321 gctggcggat gccggccacg gtaaggatca ggaaggccac gcctgacagg aacacgcagc
     1381 ccagggccgt ctgccacggc acgcccatgc cgatcacgac ggtgtaggtg aaataggcgt
     1441 tcagccccat gccgggggcg agagcgatgg ggtagttggc gatcagcccc atcaggatgc
     1501 agccgatgcc ggccgagatg caggttgccg ccgccacggc cgccacgggc atgccggcct
     1561 ggcccaggat cgccggattg acgatgacga tataggccat ggtcaggaag gtggtgaagc
     1621 cggccagcgc ctcggtgcgc aggctcgtgc cctgggcctt cagcccaaac tggcgttcca
     1681 ggaaactcat caactcaccc ccgactctcg cggtggagtt tgtccaagcg cttccggaac
     1741 gcaagcgctg tcggtcagtc cttcgtcgga tcggccgcgt ttggatcgcc ataggtcagg
     1801 gagacgaaca cgtcctccag atccgggtct tcggtggcga tatccttgat gtgcatgccg
     1861 ctggcgcgca cggccgccag cacctgctcg acgctggact gaccggtgcg atagctgacc
     1921 gagaaggcgc cgccggcccg gatcttcgtc tcgaagccgg ccagcatcgg ggccgcggtt
     1981 acgggctctt ccggcgtgac caccacattg cggctgtcca tgcgcttgag cagggtggcc
     2041 gtaggctcgc aggccaccac ctcgccgcga ttgacgatgg cgatctggtc gcac
//
LOCUS       DAOMEO010000577         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5582_length_2089_cov_2.451819, whole genome shotgun sequence.
ACCESSION   DAOMEO010000577 DAOMEO010000000
VERSION     DAOMEO010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5582_length_2089_cov_2.451819"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="gltX"
                     /locus_tag="PLH31_12480"
     CDS             complement(<1..808)
                     /gene="gltX"
                     /locus_tag="PLH31_12480"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQR90047.1"
                     /translation="MSNPSSTDRAVVTRFAPSPTGFLHIGGARTALFNWLYARHTGGK
                     FLLRVEDTDRERSTEAAVAAIFEGLDWLGLTSDEEPVFQHTRAPRHVEVVEQLLERGR
                     AYRCWMTVEELEVAREKARAEGHAIRSPWRDAPPPADPSAPHVIRFKGPAEGETLVDD
                     LVKGPVTFKNTDLDDLVLLRADGAPTYNLAVVVDDHDMGVTHVIRGDDHLNNAARQTL
                     IYQAMDWDLPAFAHIPLIHGPDGAKLSKRHGAQAVGEFADMGYIPEGLRNY"
     gene            924..>2089
                     /locus_tag="PLH31_12485"
     CDS             924..>2089
                     /locus_tag="PLH31_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQR90048.1"
                     /translation="MFRVIRDETSANLDRWVLWSPVWFGLGAAGYLEAPVEPPLTLLV
                     LLAGSGVALWWLSRASPRILIVLAALLAFMMAGGLAAKIRSDRVAAPVIDGERAPRRL
                     EGFVVDVVSPGAGGPRLLIAPVAIGGLAPEATPKRVRVTIDAIDRVEPGDAVRFRAIL
                     GPPPPPAAPGSYDFARDAWFNAVGAVGFSLGDISLTELEPPPWRLRVTMAVNAFRWRL
                     AERLVAHMGPESGGIGAAMVTGHEAWITPEQTEAMRASGLAHILSISGLHMAIVGGFV
                     FGVTRLVIAAWPWMALRAPGKKIAAVTGLVAVAIYLVISGAPPPAERAAITASVAFAA
                     ILFDRQAVTLHGLALAALIILARQPEAAGAPGFQMSFAATAALIALAEAWPRPMR"
BASE COUNT          276 a          687 c          769 g          357 t
ORIGIN      
        1 gatagttgcg caggccctcg gggatgtagc ccatgtcggc gaactcgccg acggcctggg
       61 cgccgtggcg cttggacagc ttggcgccgt cggggccgtg gatcagcggg atgtgggcga
      121 aggccggcag gtcccagtcc atcgcctgat agatcagggt ctggcgggcg gcgttgttga
      181 ggtggtcgtc gccccggatc acgtgggtga cgcccatgtc gtggtcatcg accaccaccg
      241 cgaggttgta ggtcggcgcg ccgtcggcgc gcagcaggac gagatcgtcc agatcggtgt
      301 tcttgaaggt cacgggaccc ttgaccaggt catcgaccag ggtctcgccc tcggccgggc
      361 ccttgaagcg gatcacgtgc ggcgccgacg ggtcagctgg cggcggggcg tcgcgccagg
      421 gcgagcgaat ggcgtggcct tcggcgcggg ccttttcgcg ggcgacctcc agttcctcga
      481 cggtcatcca gcagcgatag gcgcggccgc gctccagcag ttgctccacc acctcgacgt
      541 gacgcggcgc gcgggtgtgc tggaacaccg gctcctcgtc cgaggtcagg cctagccagt
      601 ccagaccctc gaagatcgcg gcgacggcgg cttcggtcga gcgctcacga tcggtgtctt
      661 cgacgcgaag aagaaacttt ccacccgtat ggcgtgcata taaccagttg aacagcgctg
      721 tgcgcgcgcc gccgatatgc aggaagccgg tgggcgaggg ggcgaagcgg gtgaccacgg
      781 cgcggtcggt ggaggagggg ttcgacatcg aactcaatgc tgaaaggcag gacgccgccg
      841 agacggccgg gatcgcgcgc cgaggcgcgg cttgggtcgc gcgtcttagc acggcggttc
      901 gcgcgtggcc tagcccggcg gcgatgttcc gcgtcatccg cgatgagacg agcgccaatc
      961 tggatcgctg ggtcctgtgg agtccggtct ggttcgggtt gggcgcggcc ggctatcttg
     1021 aagcgccagt cgagcccccg ctgacgctcc tggtgctcct ggccgggagt ggggtggccc
     1081 tgtggtggct atcgcgcgct tcaccgcgaa tcctgatcgt tctggctgcc ctgcttgcct
     1141 tcatgatggc cggtgggttg gcggcgaaga tccgcagcga tcgggtcgcc gcgcccgtca
     1201 ttgacgggga acgcgcgccg cgccgcctgg agggcttcgt ggtcgatgtc gtcagtccgg
     1261 gcgcgggcgg acctcgactg ctcatcgcgc ccgtcgccat cggtggtctc gcgccggagg
     1321 ccacacccaa acgggtccgg gtgaccatcg acgccatcga tcgggtcgag ccgggcgacg
     1381 ccgtgcggtt tcgggcgatt ctgggaccgc cgccgccgcc ggccgcgcca ggttcgtacg
     1441 acttcgcccg cgacgcctgg ttcaacgcgg tcggggccgt cgggttttcg ctgggcgaca
     1501 tcagtctcac agagctggag ccgccgccgt ggcgactgcg ggtcacgatg gccgtcaacg
     1561 cctttcgctg gcgtctggcc gagcggctcg tcgcccatat gggcccggaa agcggcggta
     1621 tcggtgcggc catggtcacc ggccacgagg cctggatcac gccggaacag accgaagcca
     1681 tgcgcgcctc gggtctggcg catatcctgt cgatctcggg cctgcacatg gccatcgtgg
     1741 gcggcttcgt ctttggcgtg acccgtcttg tgatcgcggc ctggccctgg atggccctgc
     1801 gtgcgccggg caagaagatc gccgcggtga ccggcctggt cgcggtggcc atctatctgg
     1861 tgatctccgg cgcgccgccg ccggccgagc gcgcggccat caccgccagc gtggccttcg
     1921 cggccatttt gttcgaccgg caagcggtga cactgcacgg cctggccctg gccgcgttga
     1981 tcatcctggc gcgtcagccc gaggccgccg gcgcgccggg atttcaaatg tccttcgcgg
     2041 ccaccgcagc cctgatcgcc ctggccgaag cctggccaag gccgatgcg
//
LOCUS       DAOMEO010000578         2085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5598_length_2085_cov_2.811330, whole genome shotgun sequence.
ACCESSION   DAOMEO010000578 DAOMEO010000000
VERSION     DAOMEO010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_2085_cov_2.811330"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLH31_12490"
     CDS             <1..289
                     /locus_tag="PLH31_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90049.1"
                     /translation="RKTVPDCIKLTPYNIEDLEWMPPSCAYRRLHEGKTLPDWHPLVT
                     GDPDSTHKQGISVRDQTVSELSFEVAEDAMDFVATDLMADRGDEVYDPDGD"
     gene            668..1114
                     /locus_tag="PLH31_12495"
     CDS             668..1114
                     /locus_tag="PLH31_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90050.1"
                     /translation="MAQRSEEIEAALAYVQDLVTAYYVEAAKEACETTQSDPPVARLR
                     TAEQMARAGRYLKLFRETGRRAVAAITALPSRARSSDPEETPMNDRPDWTPERVAELH
                     ADVRRRMAKLVASRDIKHVAEDGAAGPGRGVPSQPPTNRAPPAPAD"
     gene            1182..>2085
                     /locus_tag="PLH31_12500"
     CDS             1182..>2085
                     /locus_tag="PLH31_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003169090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HQR90051.1"
                     /translation="MADLIGQPLSIALVGPTFHDVREVMIEGPSGLKALSPLDDRPRW
                     EATRKRLIWNSGAVAYAFSAEDPDSLRGPQFHAAWADEFCAWPKPAETLAMLRFGLRL
                     GEDPRLVVTTTPRPIRALKTLIAEPGVAVTRAGTAANADNLAPAFLSTLQALYGGTRL
                     AAQELDGVIVEDEGGLFRAEDLARCRGARPARFDRVVVAVDPPATATGDACGIVVVGR
                     LEGRAFVLADRSAKGLSPNGWARRAVIAAQEFDAHALVAEANQGGDMVRAVLVQADPP
                     CAVKLVRASLGKRARAEPVAALYEQ"
BASE COUNT          337 a          731 c          703 g          314 t
ORIGIN      
        1 ccgcaaaacg gtgcccgact gcatcaagct gaccccctac aatatcgagg acctggagtg
       61 gatgcccccc tcctgcgcct atcgccgcct gcatgagggc aagaccctgc ccgactggca
      121 cccgctggtg acgggcgatc ctgacagcac ccacaagcag ggcatttcgg tgcgcgacca
      181 gaccgtcagc gagctgtcgt tcgaggtggc cgaggacgcg atggattttg tggcgactga
      241 cctgatggcc gatcgcgggg acgaggttta cgacccggat ggggactgac agggcggcgc
      301 agaccccctc agtcgctccg cgacagctcc cccaagaggg gagcggccaa caaagcgcga
      361 cctagctcct ccccctctgg gggaggtggc ccagagggcc ggagggggct agcgccgcgc
      421 atcagagtta tccacaggct cttgcgcctt cagatcctat cgcagagacc gcataatccc
      481 gcttgctcaa cgccaccccg ttatggcgca ggatgtcctg ggccttgcca gcgagaagac
      541 cccgactacg gggataagct gccctctatc ctctgcggat agcgaatcga acctcataaa
      601 atcaacagct tataactttc tcgcacctct ggatcagagg cgcccgctgg tcccgtatct
      661 ttgtgtcatg gcccagagaa gcgaagagat cgaagccgcc cttgcctacg tgcaggacct
      721 ggtcaccgcc tactatgtcg aggcggccaa ggaggcgtgt gagaccaccc agagcgaccc
      781 acccgtggct cgcctgagga ccgccgagca gatggcgcgc gccggtcgat acctgaaact
      841 gttccgcgag accggacgac gggcggtggc ggcgatcacc gccctgccgt cgcgtgcgcg
      901 ctcgtcggac cccgaggaga ccccgatgaa tgaccgaccc gactggacac ccgaacgtgt
      961 cgccgaactt catgccgacg tccgccgacg catggctaag cttgtcgctt cccgagacat
     1021 taaacatgtg gctgaagacg gcgccgccgg acctggtcgc ggcgtgcctt cccagcctcc
     1081 gaccaaccga gccccaccag cgcccgccga ctgacccctg gtcggcctgg ctattcctgg
     1141 ggggacgcgg tgcgggcaag accttcgccg gggccagctg ggtggccgac ctcatcggtc
     1201 agccgctgag catcgccctg gtgggcccca ccttccacga cgtgcgcgag gtgatgatcg
     1261 aggggccttc gggactcaag gcgctctcgc cgctcgatga tcgcccccgc tgggaagcga
     1321 cccgcaagcg gctgatttgg aatagcggcg ccgtcgccta cgccttctcg gccgaagacc
     1381 ccgactcctt acgcggcccg cagttccacg ccgcctgggc cgacgagttc tgcgcctggc
     1441 ccaagccggc cgagaccctg gccatgctgc ggtttggcct gcggctgggc gaggaccccc
     1501 ggctggtggt cacaaccacg ccccggccga tccgcgccct gaagaccctg atcgccgagc
     1561 ccggcgtcgc ggttacccgg gccggtacgg cggccaatgc cgacaacctg gcgccggcgt
     1621 tcctgtcgac cctgcaggcg ctctatggcg gcactcgcct ggcggcccag gagctggacg
     1681 gcgtcatcgt cgaggacgag ggcggcctgt tccgcgccga ggacctggcg cggtgtcgcg
     1741 gggcgcgtcc cgcgcgcttc gaccgggtgg tggtggcggt cgatccgccg gccacggcaa
     1801 cgggggacgc ctgcgggatc gtggtggtcg gcaggctgga ggggcgcgcc ttcgtgctgg
     1861 ccgaccgctc ggccaagggg ctttccccca atggctgggc gcgacgcgcg gtgatcgccg
     1921 cgcaggagtt cgacgcccac gccctggtcg ccgaagccaa ccagggcggc gacatggtgc
     1981 gcgccgtgct ggtccaggcc gacccgccct gcgcggtgaa actggtgcgg gcctcgctgg
     2041 gcaaacgggc gcgggccgaa ccggtggcgg ccttgtacga gcagg
//
LOCUS       DAOMEO010000579         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5601_length_2084_cov_2.860030, whole genome shotgun sequence.
ACCESSION   DAOMEO010000579 DAOMEO010000000
VERSION     DAOMEO010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_2084_cov_2.860030"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="tolB"
                     /locus_tag="PLH31_12505"
     CDS             <1..665
                     /gene="tolB"
                     /locus_tag="PLH31_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518710.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HQR90052.1"
                     /translation="QELTYMALRPTGSSIYLLNLETSRQETVGKFPGMVFAPRFSPDG
                     AKVAFSVEKGGNSDVYVLDLRSRQSTRITTDPSIDTSPSFSPDGSKIVFNSDRGGQAQ
                     LYVMNADGSGVRRISYGGGRYTTPVWSPRGDFIAFTKQAGGQFHIGVMRTDGSDERLL
                     TTSYLDEGPTWAPNGRVLMYFRETQAGTPRLWTVDVTGRILRPAAYQGQASDPAWSPL
                     LD"
     gene            838..1401
                     /gene="pal"
                     /locus_tag="PLH31_12510"
     CDS             838..1401
                     /gene="pal"
                     /locus_tag="PLH31_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQR90053.1"
                     /translation="MRRNWMNFDTKRAARLALIGLAAASLAACASRPKPAGPAPVAPS
                     EPSSPSAPYTPPPSPVAQGAIPGSVQDFVINIGERIYFDTDEYVVRSDAQPVLAAQAQ
                     WLNRYPAVRVRIEGNADERGTREYNLALGARRANAVRDFLVSQGVSGARIETLSYGKE
                     RPIDGGSSEDAWAKNRNARTAITDGAR"
     gene            1580..>2084
                     /locus_tag="PLH31_12515"
     CDS             1580..>2084
                     /locus_tag="PLH31_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQR90054.1"
                     /translation="MTLKSILLAALLASTALPALAQTPLPQDPLDDRSVRRVERMEKV
                     VRELRSIVFQGRDTGKPVVVQPAETEAQILTLGERITDLEQTLQKVNGQNEVLTHDLD
                     QARRDSTEQKGRAEALEARLAALEAKLTTMETAAVQVAAETPAPPAPPADPAVAFKQA
                     RQFLLDGD"
BASE COUNT          347 a          732 c          657 g          348 t
ORIGIN      
        1 gccaggagct gacctatatg gccctgcggc ctaccgggtc ttcgatctac ctgctgaatc
       61 tcgagaccag ccgccaggaa acggtgggca agttcccggg catggtgttt gcgccgcgct
      121 tctcgcccga tggtgccaag gtggccttct cggtcgagaa gggcggcaat agcgacgtct
      181 atgtgctgga cctgcgcagc cgccagtcga cccgcatcac gaccgatccc tcgatcgaca
      241 cctcgccctc cttctcgccg gacggcagca agatcgtctt caattccgac cggggcggcc
      301 aggcccagct gtatgtgatg aacgccgatg gctcgggcgt gcgccgcatc tcctacggcg
      361 gcggtcgcta caccacgccg gtgtggagcc cgcgcggcga tttcatcgcc ttcaccaagc
      421 aggctggcgg ccagttccat atcggcgtga tgcgcactga cggcagcgac gagcgcttgc
      481 tgaccaccag ctatctggat gaaggcccca cctgggcgcc gaacggccgt gtgctgatgt
      541 atttccgcga gacccaggct ggcacgccgc gcctgtggac cgtggatgtc accggccgca
      601 tcctgcgtcc ggccgcctat cagggtcagg cctccgatcc ggcctggtcg cccctgctcg
      661 actgatttga cccgccagcg aaccttttgc gcagtggggg cgttactgtg acggttcgcc
      721 ttgcaagtga atcaaggtcg cgacagggtc atctgacgtc attgtgaggc ttggtcaggc
      781 gttttcattg tcccgccaag gtcattggcg ggtatccatc aggtccgaac cgaggttttg
      841 aggagaaact ggatgaactt cgacacaaag cgcgccgcta gactggcgct gatcggtttg
      901 gcggccgcat cgctcgccgc ctgcgcttcg cgtccgaagc cggccggccc cgcccccgtc
      961 gcgccgagcg agccctccag ccccagcgct ccctatacgc cgccgccgtc gccggtcgcc
     1021 cagggcgcca ttcccggctc ggtgcaggac ttcgtcatca atatcggcga gcgcatttat
     1081 ttcgacacgg acgaatatgt cgtgcgcagc gacgcccagc ccgtgctcgc cgcccaggcg
     1141 caatggctga accgctatcc ggccgtgcgt gtccgcatcg aaggcaatgc cgacgaacgc
     1201 ggcacccgcg aatacaacct ggccctgggc gcccgccgcg ccaacgccgt ccgcgacttc
     1261 ctggtcagcc agggcgtgtc gggcgcgcgc atcgagaccc tgtcctacgg caaggagcgc
     1321 ccgatcgacg gcgggtcgtc ggaagacgcc tgggccaaga accgcaacgc ccgcaccgcc
     1381 atcaccgacg gcgcgcgcta aaatcataag gaacccttcc cccttgatgg gggaagggca
     1441 gggatggggg tgatgcggcc gttggtccgt ccagcgccgc ttcaccccca cccaaccctc
     1501 ccccatcaaa gggggagggc ttttcttttc gagatttcgt cgccccactt ttgtccgtaa
     1561 tcagggcata atcgccgaca tgacgctgaa atccatcctg cttgccgccc tgctggcctc
     1621 tacagccctc ccggccctgg cccagacgcc tctgccccaa gatccgctgg atgaccgctc
     1681 ggtccggcgc gtcgagcgca tggaaaaggt ggttcgcgaa ctgcggtcga tcgtctttca
     1741 gggccgcgac acgggcaagc cggtggtcgt gcagccggcc gagaccgagg cccagatcct
     1801 gaccctgggc gagcgcatca ccgatctgga acagaccctg cagaaggtca acggccagaa
     1861 cgaggtcctg acccatgacc tggaccaggc ccgccgggac tcgaccgagc agaaggggcg
     1921 cgccgaggcg ctggaggccc gtctggcggc gctggaggcc aagctcacca cgatggaaac
     1981 cgctgcggtc caggttgccg ccgagacgcc ggccccgccc gcgccgccgg ccgatccggc
     2041 cgtggccttc aagcaggcac ggcagttcct gctggacggc gact
//
LOCUS       DAOMEO010000580         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5613_length_2080_cov_2.945185, whole genome shotgun sequence.
ACCESSION   DAOMEO010000580 DAOMEO010000000
VERSION     DAOMEO010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_2080_cov_2.945185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLH31_12520"
     CDS             <1..1221
                     /locus_tag="PLH31_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQR90055.1"
                     /translation="VLGAGMMGAGIAYVQAMAGIESVLIDQTQEAADKGKGYAEALVK
                     KAVSRGKMTAEKGEAILALITPTTDYALVKGSDLVVEAVFENREVKADVTKKAEAQLA
                     DTAIFGSNTSTLPITGLAKASVRPASFIGIHFFSPVDKMGLVEIIMGEQTSQEALAKS
                     IDYVLKIKKTPIVVNDSRGFYTSRCFGTFVQEGLEMMAEGIAPAIIDNVGRATGMPRG
                     PLEMNDDVALDLSYKIGEQTKKDLGDKYEERPFAPVLAKMVVELGRLGRKNGKGFYDY
                     PEGGQKKIWPGLADLMPVTTKDADAALIEEIRTRLLYRQAVEAARCFEEGVITDPREA
                     DVGAILGWGFAPWTGGPVSLIDGIGLKTFVEGCDALAQKFGARFAPPKLLREMAEKGE
                     TFYSRFGVKTKAAA"
     assembly_gap    1241..1250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1373..2008
                     /locus_tag="PLH31_12525"
     CDS             1373..2008
                     /locus_tag="PLH31_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90056.1"
                     /translation="MPAALALIADRPARRRGRAMPWIVLASLGAHLLILGGLVAGVQI
                     MPPLDEPPVLSVELLRPAPPVPPELETAQPPQAAATRSVAPMPARVSPSPFVAPAPAP
                     PAVAKVPPGFKANGLTGGGEALRQTARDAVGCRSGDLLALTRAERSACAETLGEKNKN
                     RPAMYAVIDPDKKAAFDGACKKDDDWCLYRAGKGPYPGILALGRKKKRDDW"
BASE COUNT          377 a          705 c          683 g          305 t
ORIGIN      
        1 gttctgggcg ccggcatgat gggcgcaggc attgcctacg tccaagccat ggccggcatc
       61 gagagcgtgc tgatcgacca gacgcaggaa gccgccgaca agggcaaggg ctatgccgaa
      121 gccctggtca agaaggccgt ctcgcgcggc aagatgaccg ccgagaaggg cgaagcgatc
      181 ctggccctga tcaccccgac cacggactac gccctggtca agggttcgga cctggtggtg
      241 gaagccgtgt tcgagaaccg cgaagtcaag gccgacgtca caaagaaggc cgaagcccag
      301 ctggccgata ccgccatctt cggctccaac acctcgaccc tgccgatcac cggcctggcc
      361 aaggccagcg tccgtccggc ctcgttcatc ggcatccact tcttctcgcc ggtcgacaag
      421 atgggcctgg tcgagatcat catgggcgaa cagaccagcc aggaagccct ggccaagtcg
      481 atcgactacg tcctgaagat caagaagacc ccgatcgtcg tcaatgacag ccgcggcttc
      541 tacacgtcgc gctgcttcgg caccttcgtg caggaaggcc tggagatgat ggccgagggc
      601 atcgccccgg cgatcatcga caatgtcggc cgcgccaccg gcatgccgcg cggtccgctg
      661 gagatgaacg acgacgtcgc gctggacctc tcctacaaga tcggcgagca gaccaagaag
      721 gacctcggcg acaagtacga agagcgtccg ttcgcccccg tgctggccaa gatggtggtc
      781 gagctgggtc gcctgggccg caagaacggc aagggcttct atgactatcc ggaaggtggt
      841 cagaagaaga tctggccggg cctggctgac ctgatgcccg tcaccaccaa ggacgccgac
      901 gcggccctga tcgaggagat ccgcacgcgg cttctctatc gccaggccgt cgaagccgcc
      961 cgctgcttcg aggaaggcgt catcaccgac ccgcgcgaag ccgatgtcgg cgcgatcctg
     1021 ggctggggtt ttgcgccctg gaccggcggt cccgtcagcc tgatcgacgg cattggcctg
     1081 aagaccttcg tcgaaggctg cgacgccctg gcccagaagt tcggcgcgcg ctttgcgccg
     1141 ccgaagctgc tgcgcgaaat ggccgagaag ggcgaaacct tctacagccg cttcggcgtc
     1201 aagacgaagg cggcggcgta agcctcttcc cgcgttgaaa nnnnnnnnnn ggagagggcc
     1261 cggcggcgac gccgggccct tttcatttca gccgggagac gttgcggcca tgcttgcgcc
     1321 gcaccggctt tctatctgag aggctcgttt tgaacgggcg agacagacac cgatgccggc
     1381 ggccctcgcc ctgatcgccg acaggcccgc gcgacggcgg ggcagggcca tgccgtggat
     1441 cgtgctggcc tcgctgggcg cgcacctgct gatcctgggc ggcctcgtcg ccggggtgca
     1501 gatcatgccg ccgctggacg agccgccggt tctgagcgtg gagctgttgc ggcctgcccc
     1561 gcccgttccg ccagagctgg aaaccgccca accgccacag gcggcggcga cccgatccgt
     1621 agcgcccatg cctgcccgtg tctcgccaag cccgtttgtc gcgcccgccc cggcgccacc
     1681 cgccgtcgcc aaggtcccgc ccggcttcaa ggccaatggc ttgaccggcg gcggcgaagc
     1741 cctacgccag acggcccgcg acgccgtcgg ctgccgcagc ggcgacctgc tggccctgac
     1801 cagggccgaa cgctccgcgt gcgccgagac gctgggcgag aagaacaaga accgcccagc
     1861 gatgtatgcg gtgatcgatc ccgacaagaa ggccgccttc gacggagcct gcaagaagga
     1921 cgacgactgg tgcctgtatc gcgccggcaa gggtccctat cccggcatcc tggccctggg
     1981 gcgaaagaag aagcgagacg actggtgagt catggcgccg tccccgcaag aggcctaggc
     2041 tgacgccatg cgaaactccc tcaccagcga tgatcgggtc
//
LOCUS       DAOMEO010000581         2079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5620_length_2079_cov_2.451087, whole genome shotgun sequence.
ACCESSION   DAOMEO010000581 DAOMEO010000000
VERSION     DAOMEO010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_2079_cov_2.451087"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /gene="msrA"
                     /locus_tag="PLH31_12530"
     CDS             <1..347
                     /gene="msrA"
                     /locus_tag="PLH31_12530"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQR90057.1"
                     /translation="DPKAVSYETLLKTFWENHDPTQGMRQGNDIGTQYRSGIYVSSDA
                     QASAAVASKEAYDQALSAKGLGAITTEIAAAGPFYFAEDYHQQYLAKNPGGYCGIGGT
                     GVTCPIGVGVEA"
     gene            344..694
                     /locus_tag="PLH31_12535"
     CDS             344..694
                     /locus_tag="PLH31_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQR90058.1"
                     /translation="MSPQAFDAACRALPAVTMDIQWGSDQVYKVGGKMFAVMGGPVAQ
                     DGVSFKVSDVAFEVLTETGRAIPAPYLARAKWVKFDDLAALDEAEVVDWLTSAHGLVA
                     AKLTKKLRAELGLA"
     gene            complement(691..>1241)
                     /locus_tag="PLH31_12540"
     CDS             complement(691..>1241)
                     /locus_tag="PLH31_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HQR90059.1"
                     /translation="ASFFASSNLILIAAAAGVLFGGDTTLKSIEGLAVLAPATQTLFE
                     LKLGLVLFALARGLLDFIWAIRQMNYCLAAIGATPMWASPSTLAEYAEATGGILNPAL
                     SAFNAGVRGYYFALAGAAWLLGPYAFIAATLGALALLLWRQRRSRASAAVRKVRQILE
                     RQPVVHGPHMRHAERDPPVDTL"
     assembly_gap    1242..1251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1252..1368)
                     /locus_tag="PLH31_12545"
     CDS             complement(<1252..1368)
                     /locus_tag="PLH31_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HQR90060.1"
                     /translation="MPIIDIVALALFVFAWLFYEPLLKRLGEGGHLINTDMTV"
     gene            complement(1409..>2079)
                     /locus_tag="PLH31_12550"
     CDS             complement(1409..>2079)
                     /locus_tag="PLH31_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90061.1"
                     /translation="DTLLLFATGAAKAQGAPKANTLAMFTEHPREAFAIIGLTAAGSL
                     AFYAYTTYMLKFLTNTTGFSKETAGAVNLATLVGFMLIQPLFGWMSDRWGRRRMLIFA
                     FGGGAILAWPIFSMVARATDPVVAFMLIFAALVVQSAYTSISAVVKAELFPTHVRALG
                     VALPYALGNAVFGGTAEFVALWFKSIGLESGFYVYLAGMMAMGAVIAFALRDSGKHSR
                     ILED"
BASE COUNT          394 a          639 c          726 g          310 t
ORIGIN      
        1 ttgatccgaa ggcggtcagc tacgagacgc tgctcaagac cttctgggag aaccatgacc
       61 ccacccaggg gatgcgccag ggcaacgaca tcggcaccca gtatcgttcg ggcatctatg
      121 tctcgagcga tgcgcaggcg tccgcggccg tggcttcgaa ggaagcctac gaccaggcgc
      181 tttcggccaa gggtctgggc gcgatcacca ccgagatcgc cgccgccgga ccgttctact
      241 tcgccgagga ctatcaccag cagtacctgg ccaagaaccc gggcggctat tgcggcattg
      301 gcggcacggg cgtgacctgc ccgatcggcg tgggcgtcga ggcttgagcc cacaagcctt
      361 cgacgccgcc tgcagggcgt tgccggcggt gacgatggat atccagtggg gctccgacca
      421 ggtctacaag gtgggcggca agatgtttgc ggtgatgggc ggcccggtcg cgcaagacgg
      481 ggtgtcgttc aaggtctcgg acgtcgcctt cgaggtgttg accgaaaccg gccgcgcgat
      541 ccccgcgccg tatctggcgc gggccaagtg ggtgaagttc gacgatctgg ccgcactcga
      601 cgaggccgag gttgtcgact ggctgaccag cgctcacggg ctggtcgccg ccaagctgac
      661 caagaagctg cgggcggaac tgggcctggc ctagagcgta tcgaccggcg ggtccctttc
      721 ggcgtggcgc atgtgcgggc cgtggaccac cggttgccgt tccaggatct ggcgcacctt
      781 gcggacggcg gccgaggcgc ggctacgacg ctggcgccag agcaacaggg ccagggcgcc
      841 cagcgttgcg gcgatgaagg cgtagggccc cagcagccag gccgccccgg ccagggcaaa
      901 atagtatcca cggacgcccg cgttgaaggc tgacagggcg gggttgagga tgccgccggt
      961 cgcctcggca tattccgcca gggtggacgg tgaagcccac atcggcgtgg cgccaatggc
     1021 ggccaggcaa tagttcatct ggcgaatggc ccagatgaag tccagcagac cgcgcgccag
     1081 ggcaaacagc accagcccca gtttcagctc gaacagggtt tgggtggcgg gggccaggac
     1141 ggccagacct tcgatgctct tgagcgtggt gtcgccgcca aacagcacgc ctgctgcggc
     1201 cgcgatcagg atcaggttgg atgaggcgaa gaagctggct gnnnnnnnnn ncaccgtcat
     1261 gtcggtgttg atcaggtgtc cgccttcacc cagccgcttg agcagcggtt catagaacag
     1321 ccaggcgaac acgaagaggg cgagggcgac gatgtcgatg atcggcatgg ccatagctta
     1381 aggctggaac gggattcgac ggaagaactc agtcctccag aatgcgactg tgcttgccgc
     1441 tgtcgcgaag cgcaaaggcg atcacggcgc ccatcgccat catgccggcc agatagacat
     1501 agaagccgct ctccaggccg atgctcttga accatagggc cacgaactcg gcggtgccgc
     1561 cgaacacggc gttgccgagg gcgtagggca gggccacgcc cagggcgcga acatgggtcg
     1621 ggaaaagctc ggccttcacc actgcgctga tcgaggtata ggccgactgc accaccaggg
     1681 cggcgaagat cagcatgaac gccacaacag ggtcggtcgc cctggccacc atcgaaaaga
     1741 tcggccaggc caggatcgcg ccgccgccaa aagcgaagat cagcatccgc cgccggcccc
     1801 accggtcgga catccagcca aacagcggct ggatcagcat gaagccgacc agcgtcgcca
     1861 ggttcaccgc cccggccgtc tccttggaga agccggtggt gttggtcagg aacttcagca
     1921 tgtaggtcgt gtaggcatag aaggccagag agccggcggc cgtcaggccg atgatggcga
     1981 aggcctcacg cgggtgctcg gtgaacatcg ccagggtgtt ggccttgggc gcgccctggg
     2041 ccttggcggc gccggtggcg aacagcagca gggtgtcgg
//
LOCUS       DAOMEO010000582         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5637_length_2074_cov_2.796929, whole genome shotgun sequence.
ACCESSION   DAOMEO010000582 DAOMEO010000000
VERSION     DAOMEO010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5637_length_2074_cov_2.796929"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="gatB"
                     /locus_tag="PLH31_12555"
     CDS             <1..1422
                     /gene="gatB"
                     /locus_tag="PLH31_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517891.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQR90062.1"
                     /translation="PGQGGIVIGTGGAAVGFGAGPNEQVSLVDAAMPGMLPVLNGFCV
                     EQAVKTGLGLKAQINLKSRFDRKNYFYPDLPQGYQISQFDQPIVGEGVVTVERDDGTT
                     FDVRIERLHLEQDAGKSLHDQDPNATYVDLNRAGTALMEIVSKPDMRTSEEAAAYVKK
                     LRTILVYLGTCDGDMEKGNLRADVNVSVCRPGDYEKFRETNDFKHLGTRCEIKNVNSY
                     RYIQQAIEYEARRQIEILEDGGKVDQETRLFDPTKLETRSMRSKEEAHDYRYFPDPDL
                     LPLILDADWVKTIEETLPELPDAKKARLQSQYGLSAYDAGVLIIESDRADYFEAAAKG
                     RDAKLVSNWVTNELLAKLSAGGHEIANSPLPASDIAEMVELIENGTISSKIAKELFEH
                     MWAGEGRPSEIVQKRGLVQINDTGAIEAAIDKLIAANPDKAEAVKEKPQAIGWFVGQV
                     MKETGGKANPATVNELLKSKLGL"
     gene            complement(1470..1943)
                     /locus_tag="PLH31_12560"
     CDS             complement(1470..1943)
                     /locus_tag="PLH31_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HQR90063.1"
                     /translation="MKRLLLTIAAIASVAAPMAVVATDASAQDRGRWEHRGDRGDRYD
                     RRDGRHDGRYDGRRDHRWDGGRHNGYYYNNRFHYGAPPSSYYGRPGYRPGYASWRRGA
                     YLPPYYRDRGYVVHDYHRYRLRAPPRGYYWYRTGNDYVLAAIATGLIFEVMNNGY"
BASE COUNT          410 a          660 c          673 g          331 t
ORIGIN      
        1 ccggggcaag gcggcattgt gatcggcacc ggtggcgccg ccgtgggctt tggcgctggg
       61 cccaacgagc aggtgagcct ggtggatgcg gccatgcccg gcatgctgcc ggtgctgaac
      121 ggcttctgcg tcgagcaggc ggtcaagacg ggcctgggcc tgaaggcgca gatcaatctg
      181 aagagccgct tcgaccgcaa gaactacttc tatcccgatc tgccgcaggg ctatcagatc
      241 agccagttcg accagccgat cgtcggcgag ggcgtcgtca ccgtcgagcg cgacgacggc
      301 acgaccttcg acgtgcgcat cgagcgcctg catctggaac aggacgccgg caagtcgctg
      361 cacgatcagg atccgaacgc cacctatgtg gacctgaacc gcgcgggcac cgcgctgatg
      421 gagatcgtct ccaagccgga catgcgaacc tccgaagagg ccgccgccta cgtcaagaag
      481 ctgcggacga tcctggtcta tctgggcacc tgcgacggcg acatggaaaa gggcaacctg
      541 cgcgccgacg tcaacgtctc ggtctgccgc cccggcgact atgaaaagtt ccgcgagact
      601 aacgacttca aacatctggg cacgcgctgc gagatcaaga acgtcaactc gtatcgctac
      661 atccagcagg ccatcgagta tgaggcccgt cgccagatcg agatcctgga agacggcggc
      721 aaggtcgacc aggaaacccg cctgttcgat ccgaccaagc tcgagacccg ttcgatgcgg
      781 tccaaggaag aggcgcacga ctatcgctac ttccccgatc ccgatctgtt gccgctgatt
      841 ctcgacgccg actgggtcaa gacgatcgaa gagaccctgc cggagctgcc ggacgccaag
      901 aaggcgcgcc tgcagagcca gtatggcctg tcggcctatg acgccggcgt gctgatcatc
      961 gaaagcgacc gcgcggatta cttcgaggcc gccgcgaaag gccgcgacgc caagctggtg
     1021 tccaactggg tgaccaatga actgctggcc aagctgtcgg ctggcggcca cgaaatcgcc
     1081 aactcgccgc tgccggcttc ggacatcgcc gagatggtcg agctgatcga gaacggcacc
     1141 atcagctcca agatcgccaa ggagctgttc gagcacatgt gggctggcga ggggcgtcct
     1201 tccgagatcg tccagaagcg cggtctggtc cagatcaacg ataccggcgc catcgaggcg
     1261 gcgatcgaca agctgatcgc ggccaatccc gacaaggccg aggccgtgaa ggaaaagccc
     1321 caggccatcg gctggttcgt cggtcaggtg atgaaggaaa ccggcggcaa ggccaatccg
     1381 gccacggtca acgaactgct gaagtccaag ctggggctct agcatgcaac agcgccccgg
     1441 aggtctccct ccggggcgct cttttaggcc tagtagccgt tgttcatgac ctcgaagatc
     1501 aggcccgtgg cgatggccgc caggacgtag tcattgcccg tccggtacca gtagtagccg
     1561 cggggcgggg cgcgcagccg atagcggtga tagtcgtgca ccacatagcc ccggtcccgg
     1621 taatagggcg gcagataggc cccgcgccgc caggacgcat agcccgggcg atagcccgga
     1681 cggccgtagt agctggacgg cggcgcgcca tagtggaagc ggttgttgta gtagtacccg
     1741 ttgtgtcggc cgccatccca gcgatggtcg cgccggccat cgtaacgacc atcatgtcgg
     1801 ccgtcccggc gatcatagcg gtcgccgcga tctccacgat gttcccagcg gccgcgatcc
     1861 tgagcggaag cgtcggtggc cacgacggcc atgggggcgg ctaccgatgc gatcgcggcg
     1921 atggtgagca acagacgttt catctcgtcc actccttctg gtcaggcggg tttcacgccg
     1981 ccaagtccct cgaccttgga gaccatcctc tgacagaccg cctgaaccgc gcctgaatgg
     2041 ggctgtcagg cgaagacgcg gtttccgcta ggtc
//
LOCUS       DAOMEO010000583         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5638_length_2074_cov_2.660723, whole genome shotgun sequence.
ACCESSION   DAOMEO010000583 DAOMEO010000000
VERSION     DAOMEO010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_2074_cov_2.660723"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLH31_12565"
     CDS             complement(<1..990)
                     /locus_tag="PLH31_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR90064.1"
                     /translation="MTTLDPSVTEGGRKPAPLAGGLSSAVLLRATKDAFVKLDPRQLT
                     SNPVIFTTWIVALLSTVSAVAAVVSHQPAGFAIQIAIWLWATVLFANLAESVAEGRGK
                     AAADSLRATRVTTKAKLIIDEKTGTIVPTPAHKLGVGEIILVEAGDVIPTDGEIIEGV
                     ASVNEAAITGESAPVIRESGGDRSAVTGGTTVVSDWIKVRVTAEAGNTFLDRMIAMVE
                     GADRRKTPNEIALAVLLAGLTLIFLIAVVTLLGLGKFSGVAIDPLVLGALFITLIPTT
                     IGGLLSAVGIAGMDRLLKVNVLATSGRAVEAAGDVDTLLLDKTGTITFGNRMAT"
     gene            complement(1018..>2074)
                     /gene="kdpA"
                     /locus_tag="PLH31_12570"
     CDS             complement(1018..>2074)
                     /gene="kdpA"
                     /locus_tag="PLH31_12570"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HQR90065.1"
                     /translation="LFATASQEAIKQLGINGGGVFNVNSAHPFENPTPLTNLITAVSI
                     NVLGWAAFFAFGRSAMARKDIRALVAAALILLSAGAAAIYATETQAPPALVAAGVDAS
                     VNMEGKEVRFGAPSTATWAAMTTGASNGSVNGMHSSLMPLGGAVTLFLMQLGEILPGG
                     IGSGIAIMVVMALLAVFVAGLMVGRTPEYLGKKVEAREIQLAMLAVLVIPLSMLGFSG
                     VAAVLPEALKGLMHAGPHGLSEILYGYTSATANNGSAFGGLTANAPWWNTTLGISMML
                     GRFAPIVAVLAIAGALAAKPKLAPSAGTLPTDGGLFIGLLIGVILILGGLQFFPALAL
                     GPIVEHFQALAAVASVH"
BASE COUNT          342 a          675 c          746 g          311 t
ORIGIN      
        1 ggtcgccatg cggttgccga aggtgatggt gccggtcttg tccagcagca gggtgtcgac
       61 gtcgccggcg gcttccaccg cgcggcccga ggtggccagc acattgacct tcagcaggcg
      121 gtccatgccg gcgatgccga cggccgagag caggccaccg atggtggtgg ggatcagggt
      181 gatgaacagc gcgcccagca ccagcgggtc gatcgccacg cccgagaact tgcccaggcc
      241 cagcagggtg accaccgcga tcaggaagat cagggtcagg ccggccagca gcacagccag
      301 ggcgatctca ttgggcgtct tgcgacggtc cgcgccctcg accatggcga tcatgcggtc
      361 gaggaaggta ttgcccgcct cggccgtgac ccgcaccttg atccagtcgg agaccacggt
      421 ggtgccgccg gtgacggccg agcggtcgcc gccgctttcg cggatcacgg gcgcgctttc
      481 gccggtgatg gcggcctcgt tgacgctggc gacgccttcg atgatctcgc cgtcggtggg
      541 gatcacgtcg ccggcctcga ccaggatgat ctcgccgacg cccagcttgt gagcgggggt
      601 cgggacgatg gtcccggtct tctcgtcgat gatcagcttg gccttggtgg tgacccgggt
      661 ggcgcgcagg ctgtcggcgg ccgccttgcc gcgcccttcg gcgacgcttt cggccaggtt
      721 ggcgaacagc accgtggccc atagccagat cgcgatctgg atcgcgaagc cggccggctg
      781 atggctgacc accgctgcga cggccgagac cgtcgacagc aaggccacga tccaggtggt
      841 gaagatcacc ggattgctgg tgagctggcg cgggtccagc ttgacgaagg cgtccttggt
      901 cgcgcgcaac agcacggcgg aggagaggcc gccggccagg ggagcgggct tgcgcccgcc
      961 ctctgtgacg gaggggtcta gggtggtcat gtcaaatcgc ctttgcgatc agctgtgtca
     1021 gtggacgctg gcgaccgcgg cgagcgcctg gaagtgctcg acgatcggac ccagcgccaa
     1081 ggcggggaag aattgcaggc cgcccaggat caggatcacc ccgatcagca ggccgatgaa
     1141 caggccgcca tcggtgggca gggtgcccgc agacggggca agcttgggct tggccgccag
     1201 ggcgccggcg atggccagca ccgcgacgat gggggcaaag cggcccagca tcatcgagat
     1261 gcccagggtg gtgttccacc acggcgcatt ggccgtcagc ccgccaaacg ccgagccgtt
     1321 attggccgtg gccgaggtgt agccgtagag aatctccgac aggccgtgcg gtccggcgtg
     1381 catcagtccc ttcagggcct cgggcaggac ggccgctacg cctgagaacc ccagcatcga
     1441 cagcgggatc accagcaccg ccagcatggc cagctggatc tcgcgggcct cgaccttctt
     1501 gcccagatat tcgggcgtgc ggcccaccat cagaccggcg acgaacactg ccagcagggc
     1561 catgacgacc atgatggcga tccccgaacc gatgccgccg ggcaggatct cgcccaactg
     1621 catcaggaac agtgtcaccg cgccgcccag cggcatcagg ctggaatgca tcccgttgac
     1681 cgagccgttg gaggcgcctg tggtcatggc cgcccaggtg gccgtcgagg gagcgccgaa
     1741 gcgaacctcc ttgccctcca tattgaccga ggcgtcgacg ccggccgcga cgagggcagg
     1801 aggcgcttgg gtctcggtgg cgtagatcgc cgccgcgccg gccgacagca ggatcagggc
     1861 cgccgccacc agggcgcgga tgtccttgcg ggccatggcg ctgcggccga aggcgaagaa
     1921 cgccgcccag cccagcacat tgatcgacac cgccgtgatc aggttggtca gcggcgtggg
     1981 gttctcgaac ggatgggcgg agttgacgtt gaagacgccg ccgccattga tgcccagctg
     2041 cttgatggct tcctggctgg ccgtggcgaa cagg
//
LOCUS       DAOMEO010000584         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5643_length_2073_cov_2.396432, whole genome shotgun sequence.
ACCESSION   DAOMEO010000584 DAOMEO010000000
VERSION     DAOMEO010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_2073_cov_2.396432"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLH31_12575"
     CDS             complement(<1..620)
                     /locus_tag="PLH31_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQR90066.1"
                     /translation="MFDKILIANRGEIALRVHRACKEMGIATVAVHSEADSNSMWVRL
                     ADESVCIGPAPAAKSYLNIPSIIAACEITGAQAVHPGYGFLSENARFAEIVGAHGFTF
                     IGPKPEHIRLMGDKITAKQAVKDAGIPVVPGSDGGVSTEEEAFAAADVIGFPVLIKAA
                     SGGGGRGMKVAQTREDLAEAVSTARSEARAAFGDDTVYMERYLQKPR"
     gene            complement(626..1138)
                     /gene="accB"
                     /locus_tag="PLH31_12580"
     CDS             complement(626..1138)
                     /gene="accB"
                     /locus_tag="PLH31_12580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQR90067.1"
                     /translation="MSNPKAPADPVETPSIDARLVRKLADILKDTGLSEIEVEHAGLK
                     IRVARELTVAAAATTYVQAPQAAYAPQAASAPAPVAEAPAPAAPAARAAGDLVKSPMV
                     GTVYMSPQPGADAFIKVGDTVAAGQTLLIVEAMKTMNPISAPKAGKVVEILVGDAQPV
                     EFGEPLVVIE"
     gene            complement(1192..1635)
                     /gene="aroQ"
                     /locus_tag="PLH31_12585"
     CDS             complement(1192..1635)
                     /gene="aroQ"
                     /locus_tag="PLH31_12585"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQR90068.1"
                     /translation="MVNPIHVLSGPNLNLLGTREPEIYGKDTLDDVRARCEARAALHG
                     HSVVFRQSNHEGQLIDWVQEARVSASALVINPAGYGHTSIALLDALKTLSIPVIECHL
                     SNPAAREEFRRHTYVSLAATGIVSGFGAASYELAIEAAIGLIGAN"
     gene            1714..1914
                     /gene="thiS"
                     /locus_tag="PLH31_12590"
     CDS             1714..1914
                     /gene="thiS"
                     /locus_tag="PLH31_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HQR90069.1"
                     /translation="MRLLLNGDEREIADVFSVADLVSALGLDSRKVAVERNLEIVPRS
                     TYADTALADGDRIEIVTFIGGG"
     gene            1959..>2073
                     /locus_tag="PLH31_12595"
     CDS             1959..>2073
                     /locus_tag="PLH31_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HQR90070.1"
                     /translation="MNAHVSPDTALTSDDSWTVAGRTFTSRLIVGTGKYKDY"
BASE COUNT          339 a          643 c          722 g          369 t
ORIGIN      
        1 cgcggcttct ggaggtagcg ctccatgtag accgtgtcgt cgccgaaggc cgcgcgggct
       61 tccgaacgag cggtcgagac cgcctcggcc agatcctcgc gggtctgggc caccttcatg
      121 ccacgcccgc caccgccgga ggcggccttg atcaggacgg ggaagccgat cacatcggcg
      181 gccgcgaagg cttcttcctc ggtcgagacg ccgccatcgg aaccgggaac gacgggaatg
      241 cccgcgtcct tgacggcctg tttggcggtg atcttgtcgc ccatcagccg gatgtgttcg
      301 ggcttggggc cgatgaaggt gaagccatgg gcgccaacga tctcggcgaa gcgggcgttc
      361 tccgacagga agccgtagcc ggggtggacc gcctgggcgc cggtgatctc gcaggccgcg
      421 atgatcgacg ggatgttcag atagcttttg gcggcggggg cggggccaat gcagacgctt
      481 tcgtcggcca ggcgcaccca catcgagttg gagtcggcct cggagtggac cgccacggtg
      541 gcgatgccca tttccttgca ggcgcggtga acccggagcg cgatctcgcc ccggttcgcg
      601 atcaggatct tgtcaaacat gatcgttact cgatgacgac gagcggctcg ccgaactcga
      661 cgggctgggc gtcaccgacg aggatctcga cgaccttgcc ggccttgggg gccgaaatcg
      721 ggttcatcgt cttcatggcc tcgacgatca gcagggtctg gcctgcggcg acggtatcgc
      781 caaccttgat gaaggcgtcg gcgccgggct ggggcgacat gtagacggtg ccgaccatcg
      841 gcgacttgac cagatcgcca gcggcgcggg cggcgggcgc ggccggggcg ggagcttcag
      901 cgacgggagc gggggccgat gccgcttgcg gcgcataggc agcctgcgga gcctgaacat
      961 aggtggtggc ggctgcggcc acggtcagtt cgcgggccac gcggatcttc aggccggcgt
     1021 gttcgacctc gatttcgctc aggccggtgt ccttcaggat gtccgccagt ttgcggacca
     1081 ggcgggcgtc gatcgacggg gtctcgaccg gatcggcggg agccttggga ttggacattg
     1141 aagctacctt gttctcttgg ctcgcggcga tggaacgcct gcgggctgga cctagttcgc
     1201 gccgatcagg ccgatagcgg cctcgatcgc cagttcataa ctcgccgcgc cgaagccgga
     1261 gacgatcccc gtggctgcca gcgagacata ggtgtggcga cggaattcct cccgcgccgc
     1321 cgggttggac aggtggcact cgatcaccgg aatgctcaag gttttgagcg cgtccagcag
     1381 ggcgatcgag gtgtggccat aaccggccgg gttgatcacg agcgccgagg ccgacacacg
     1441 cgcctcctgc acccagtcga tcagctggcc ttcatggttg gactgccgga agaccacgga
     1501 gtggccatgc agagccgcac gcgcctcgca gcgcgcccgg acatcgtcca gcgtgtcctt
     1561 gccatagatc tcgggctcac gcgttcccaa caggttgaga ttggggccgc tcagcacatg
     1621 gatcgggttt accattcggc tccgccgtcg attccgaagg tcttgtatag ccggttccgc
     1681 cgggtcacaa gcagcgctcg ctcaggaggt gagatgaggc ttttattgaa tggcgatgag
     1741 cgggagatcg ccgacgtctt cagcgtcgct gatctggtgt ctgccttggg cctggactcg
     1801 cgcaaggtgg ctgtcgagcg caacctggag atcgtgccgc ggtctaccta tgccgatacg
     1861 gccctggctg acggcgaccg catcgagatc gtgacattta tcggcggcgg atgacctcca
     1921 ggtttgaaac cgcgcctgac ggggcgtaaa ccagccgcat gaacgcgcat gtttcccccg
     1981 acaccgccct gacgtccgac gatagctgga cggtcgcagg ccgcaccttc acctctcggc
     2041 tgatcgtcgg caccggcaag tacaaggact atg
//
LOCUS       DAOMEO010000585         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5648_length_2072_cov_2.884978, whole genome shotgun sequence.
ACCESSION   DAOMEO010000585 DAOMEO010000000
VERSION     DAOMEO010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_2072_cov_2.884978"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLH31_12600"
     CDS             complement(<1..213)
                     /locus_tag="PLH31_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQR90071.1"
                     /translation="MPESPVITGVTADSRKVGPGTLFAALPGTKVDGSSFAPGAIAAG
                     AAAVLGPVGMETFSVPTVKVEDPRRAY"
     assembly_gap    224..292
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(296..2062)
                     /locus_tag="PLH31_12605"
     CDS             complement(296..2062)
                     /locus_tag="PLH31_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQR90072.1"
                     /translation="MSLANLGPHGFQPGAWRWLIERVWRLEHAFERSRAAAKPEDDTR
                     IRIFFVMAFFGVCFVALALGASASALFSGGGKGPGYLASTEGARGDLVDRDGRLLAVD
                     LTHYALYVDPREVWDKPQARRALGKALPEVPAKRLDKVIFGERRTFLLGGLTPEQKDA
                     IFNLGLPGISFEEQGRRVYPLGETAAHLVGFVDSGGKGLAGAERALDKPIRASAGEGG
                     AATALSIDIRVQAALEDELRKAALEFQPRGAVGVVTNVHTGEILGMASYPDFDPNKAG
                     TATDDQKLNRAAGAVYEMGSTFKAFTVAIGLDTGVATPASTFDAREPYKLGYRTIHDY
                     HAAKAILTLVEVFKHSSNIGTAKLAVGVGAQRLSQYFTNLGLTKPAKVELLESARPLT
                     PRKWDEDAVASTSFGHGMNVTPLALAEAMGPLLNGGTRPPLTIRKLASGVRPEGPRVL
                     NEETSQQMLRIMRANVIPGEGGSGGKADVAGLSVGGKTGTGEKYDPAIRAYNHQRQVS
                     SFAAVFPTEGPIEADRYFVLILLDEPKGNARTFGFSTGGWVAAPAAGKVIERIAPFLG
                     VHRKPQIMTVSNSQNTSPGTGL"
BASE COUNT          294 a          672 c          731 g          306 t
ORIGIN      
        1 gtaggcgcgg cgggggtctt ccaccttcac ggtcgggacc gagaaggtct ccatgccgac
       61 cggacccagt acggcggcgg ccccggccgc gatggcgccg ggcgcaaagc tggagccgtc
      121 gaccttggtc cccggcaggg cggcgaacag ggttccgggc ccgaccttgc ggctgtcggc
      181 cgtcaccccg gtaatcaccg gactttcggg caggtcgcgc tgannnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntggtcaca
      301 ggccggttcc ggggctggtg ttttgcgagt tggagacggt catgatctgc ggcttgcgat
      361 gcacgcccag gaacggggcg atgcgctcga tcaccttgcc ggcggccggc gcggccaccc
      421 agccgcccgt cgagaagccg aaggtgcggg cgttgccctt gggctcgtcc agcaggatca
      481 gcacgaaata gcggtcggcc tcgatcggcc cctcggtcgg gaacaccgcc gcgaacgacg
      541 agacctgacg ctggtggtta taggcgcgga tggccgggtc gtacttttcg ccggtgccgg
      601 tcttgccgcc caccgacagg ccggccacgt cggccttgcc gccgctgccg ccttcgccgg
      661 gaatgacatt ggcccgcatg atccgcagca tctgctgcga ggtttcctcg ttcagcacgc
      721 gcggaccttc gggacgcacg cccgaggcca gcttgcggat ggtcaggggc ggacgggtgc
      781 cgccgttcag cagcgggccc atggcttcgg ccagggccag cggcgtcacg ttcatgccgt
      841 gaccaaacga ggtcgaggcc accgcatcct cgtcccactt gcgcggggtc aggggacggg
      901 ccgattccag cagttcgacc ttggccggct tggtgaggcc cagattggtg aaatactggc
      961 tcagccgctg ggcgccgacg ccgaccgcca gcttggcggt gccgatgttg gacgagtgct
     1021 tgaacacctc gaccagggtc aggatcgcct tggcggcgtg atagtcgtgg atggtgcgat
     1081 agcccagctt gtagggctcg cgggcgtcga aggtggaggc cggggtggcg acgccggtgt
     1141 ccaggccgat ggcgaccgtg aaggccttga aggtcgagcc catctcatag accgcgccgg
     1201 ccgcccggtt cagcttctgg tcgtcggtgg cggttccggc cttgttggga tcgaagtcgg
     1261 gatagctggc catgcccagg atttcgccag tgtggacatt ggtcaccacg cccaccgcgc
     1321 cgcgcggctg gaactccagg gcggccttgc gcagctcgtc ttccagggcg gcctgcacgc
     1381 ggatgtcgat cgacagagcg gtcgcggcgc cgccctcgcc ggccgaggcg cggatcggct
     1441 tgtccagggc ccgctcggcg ccggccaggc ccttgccgcc gctgtcgacg aagccgacca
     1501 ggtgggcggc ggtctcgccc agcggataga cgcggcgccc ctgctcctcg aacgagatac
     1561 ccggcaggcc cagattgaag atggcgtcct tctgctcggg cgtcaggccg cccagcagga
     1621 aggtgcgacg ctcgccgaag atcaccttgt ccagacgctt ggccgggact tcgggcaggg
     1681 ccttgcccag cgcccggcgg gcctgcggct tgtcccagac ctcccgggga tcgacataga
     1741 gggcgtagtg ggtcaggtcg accgccagca ggcggccatc gcggtccacc agatcgccgc
     1801 gcgcgccctc ggtcgaggcc agatagcccg gccccttgcc cccgcccgag aacagggccg
     1861 aggcgctggc gccgagggcc agggccacaa agcacacgcc aaagaaggcc atcacgaaga
     1921 agatgcggat gcgggtgtcg tcttcgggct tggcggcggc gcgcgagcgc tcgaaggcgt
     1981 gctccagccg ccagacgcgc tcgatcagcc agcgccaggc gccaggctga aagccgtggg
     2041 ggcccagatt ggcgaggctc atcgcggagc ct
//
LOCUS       DAOMEO010000586         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5657_length_2070_cov_2.575186, whole genome shotgun sequence.
ACCESSION   DAOMEO010000586 DAOMEO010000000
VERSION     DAOMEO010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_2070_cov_2.575186"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..1427)
                     /locus_tag="PLH31_12610"
                     /pseudo
     CDS             complement(444..1427)
                     /locus_tag="PLH31_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
     gene            complement(1536..2009)
                     /gene="greA"
                     /locus_tag="PLH31_12615"
     CDS             complement(1536..2009)
                     /gene="greA"
                     /locus_tag="PLH31_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQR90073.1"
                     /translation="MEKVPMTAGGYQTLDDELKRLKTIERPAVIAAISEARQHGDLSE
                     NAEYHAAKERQGWIEGRIAEIEDKIARAQVIDVSKLSGKQVKFGATVSVVDEDTEEEA
                     RYQIVGDHEADVKEGRISLSSPLSRAMIGKEVGEVVEVNTPGGVKAYEILKVEWL"
BASE COUNT          302 a          678 c          691 g          399 t
ORIGIN      
        1 ttgatgtatg aatcaactta atgcaacaat agactgcctt cctcgccctt gtggcgagga
       61 cccatggtga ggcttggttc tgctgtcgtc agtgcgcccc gcatctatgg gcgcgcatcc
      121 tgcttgtcac agcgagcgga cgcatgggtc ccaggcacaa ggcctgggaa ggcaagctat
      181 tgggttttgg gtttggtgcg cggtttaggc ttcgcagcag ccgttgccgt ggccgtaact
      241 gtagtcttgg ccttcgtccc ggctttcttc gcaaccggct tggtcgcggg cgcagccttc
      301 ggcttcgcgg ccggcttttc cgctaccgcc gccgtcttgc tccgaggttt ccgcgatggc
      361 cttggagcga ctggcggttc ggcgacaggc tcgaccgcct cggcctcagg cgcctcctcg
      421 acctcaacca cagtctcccc ccgcatccgc gccagcacct cgcgcgcggc gcggccggtt
      481 tcatcgacgg gctcatacgt ccagccatgg aacgcgccgc cataggggcg ggccgcgccc
      541 tggcgttcca gctcctcgtg aaagaggcgg aacttcaggc tggagccttc catcttcagg
      601 atgaacaccg gcttgcccgt cgacgcggct tcggtggcca tgttggtcga gtcctcggtg
      661 acgaggatgt agtcggcggc cgccaggaag gcgaaatagg ggttggggcc ttcgccgtcc
      721 cagaccatgc cgggcaggtg gcgcaggcgg gcggtcagca gggcgcgggc cggctctggc
      781 gtgcggcgcg agaaggtcat cagcaacgag ccgccgtcct gctccagcgg gatctggatc
      841 tggtgggcca tctgggcggc gcgttcgacg ggcaggtcaa aggccttgga cttgccgccc
      901 agcagcaccg cgacgcgcgg gcggggcaga tcgtcaagct gggccttgaa gacttcgtac
      961 tcgctggcca gccgcgcggc ggtcacccgg tgcggcgagc cggtgatggg caggatgttg
     1021 tcgccggaca atcggtcgtg ctttggcggg atcaccaggt cgaacatctg gggcgggacc
     1081 cgcggatcct ggatctggac cacataggtc ttgccgcccg accagcgctt ggcgcggatc
     1141 gacaggggca gggtggcgcg gccggcggcg atccacaggt cgggccaggg accgttgatc
     1201 ctgctttcgg gggtcagcca gcggcgcggg agcaggttca gccaccaggg caggcggccg
     1261 atacggccgg tccagccgac gcgcttgatc tcgatcgcgg cctcgatgcc ctgggtccgc
     1321 gcttcacgcg cgaccgcctc ggccaggccc acggcctggg cctcgatgcc ggcgcggccg
     1381 tccgagatcg cccagatcac gacaggaggt ttcggggggt tcttcaagac gtcatcccga
     1441 cgagcgcagt catgcagaca aggccgctcc cgaaagaggc ggccctgctc catggtctcg
     1501 ctttatggcg cgggtttggc ggcaggccta gaggcctaca gccactccac tttcaggatc
     1561 tcgtaggcct tcacgccgcc gggcgtgttg acctcgacga cttcgcccac ttccttgccg
     1621 atcatggcgc gcgacagcgg ggacgacagc gagatgcggc cctccttcac atcggcttcg
     1681 tgatcgccaa cgatctggta gcgagcctct tcctcggtgt cctcgtcgac gacgctgacg
     1741 gtcgctccga acttcacctg cttgcccgaa agcttcgata cgtcgatcac ctgcgcgcgg
     1801 gcgatcttgt cctcgatctc ggcaatccgg ccttcgatcc agccctgacg ttccttggcc
     1861 gcgtgatact cggcattttc cgagaggtcg ccatgctggc gcgcctccga gatcgcggca
     1921 atgaccgcgg gccgctcgat cgtcttcaaa cgcttcagtt catcgtcgag ggtttgataa
     1981 cccccggcgg tcatcggcac tttttccatt gcggatttta gctcggttcg ccttgggagg
     2041 attatacgtc ccgtcgctgg cgcacgcgac
//
LOCUS       DAOMEO010000587         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5661_length_2069_cov_2.666832, whole genome shotgun sequence.
ACCESSION   DAOMEO010000587 DAOMEO010000000
VERSION     DAOMEO010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_2069_cov_2.666832"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..591
                     /locus_tag="PLH31_12620"
     CDS             271..591
                     /locus_tag="PLH31_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90074.1"
                     /translation="MKRAFTIGMATLGVALLLSACGPKIEIRPAQFAVTGDRRAVLAF
                     AADQIAAGATNISISPGETRDTARATFPYARSQEIVRLAQKASEARLGWSYGGQSTRI
                     SVKL"
     gene            637..2001
                     /locus_tag="PLH31_12625"
     CDS             637..2001
                     /locus_tag="PLH31_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90075.1"
                     /translation="MSSAPSDPESQAGSRRADAVFHHLLANNLVANITNFTVWFALTF
                     WVYLETRSVFATGMVAGIYLVLTAASGFWFGSLVDHHRKKIVMLGSSLASMALYAAAL
                     AVHELSPKGAMADVAGPFLWLFIWLTMLGVIAGNIRSIALPTLVTILIPEDRRDRANG
                     LVGMVSGVGFLTTSVISGFLVAWGGMRYTLVFALALTVIVLVHLLAVRVDEPRAEPHA
                     DAAPEPRRVDIQGTMKVIAAVPGLFALILFACFNNFLGGIFMALLDAYGLSLVSVQTW
                     GLLFGFLSTAFIFSGIVISKTGLGASPLRTLLLVNLITWSVCCVFTLQSSIVLLAAGC
                     FVWMLLGPYAEAAEHTTLQKVVPLERQGRVFGFAQSVEQAASPLTAFLIGPLTQFIVI
                     PFMTDGAGAAAIGDWFGRGPARGMALVFTLAGVVGVAATVVAFNSRYYRQLSAAYARG
                     ASAP"
BASE COUNT          290 a          670 c          691 g          418 t
ORIGIN      
        1 atccccatcg ccgctgttgg gccggatcca ggcgcctccc cctgcgacgg ggacaaggag
       61 cagtatccat caggttttgg agccggggat acgcgagcgc gagaaagttg gaagtgattg
      121 attttgctgg cttcgattct ctgaacgccg aggatagagc gcgaagatcc tcgcccttca
      181 ggggaggact gtcaggcaga gtctcgccct gagattgcgt tttcgccaga aacgcgacat
      241 ggtcgcgtct gctcagacgg agatcgtcac atgaagcgcg ctttcacgat cggcatggcg
      301 acccttggcg tggccctgct gctgtcggcg tgcgggccga agatcgagat ccggcccgcc
      361 cagttcgccg ttaccggaga ccgtcgggcc gtgctggcct tcgccgccga tcagatcgcc
      421 gccggggcga ccaatatttc gatcagcccg ggcgaaaccc gggacaccgc gcgcgccacc
      481 ttcccttacg cccggtccca ggagattgtc cgactagcgc agaaggcttc ggaagcgcga
      541 ctgggctgga gctacggcgg gcaatcgacc cgtatttcgg tcaagctcta agagccccgc
      601 gttgcgccgg ccgtgatcgg ctggcaagct tggccgatgt cgagcgcgcc ttccgacccc
      661 gaaagccagg ccggttcgcg ccgcgccgat gcggtgtttc atcacctgct ggccaacaac
      721 ctggtcgcca acatcaccaa tttcacggtg tggttcgccc tgaccttctg ggtctatctg
      781 gagacccggt cggtattcgc caccggcatg gtggcgggca tctatctggt gctgaccgcc
      841 gcatcgggct tctggttcgg cagcctggtg gatcatcacc gcaagaagat cgtcatgctg
      901 ggctcaagcc tggcgtcgat ggccctgtat gcggcggcgc tggcggtcca tgagctgtcg
      961 ccgaaagggg cgatggctga cgtggccggg ccgttcctgt ggctgttcat ctggctgacc
     1021 atgctgggcg tcatcgccgg caatatccgc agcattgccc tgcccaccct ggtgacgatc
     1081 ctgattcccg aagaccggcg cgacagggcc aacggcctgg tcggcatggt cagcggcgtc
     1141 ggcttcctga ccacttcggt gatcagcggc ttcctcgtgg cctggggcgg gatgcgctac
     1201 accctggtct ttgccctggc cctgaccgtg atcgtgctgg tccatctgct ggcggtgcgg
     1261 gtggacgagc cgcgcgccga gcctcatgcc gacgcggcgc cggagccccg gcgggtggat
     1321 atccagggca cgatgaaggt gatcgccgcc gtgcccggcc tgtttgccct gatcctgttt
     1381 gcctgtttca acaatttcct gggcggcatc ttcatggccc tgctggacgc ctatggcctg
     1441 tcgctggtct cggtccagac ctgggggctg ctgtttggct tcctgtcgac agccttcatc
     1501 ttcagcggca tcgtgatctc gaagaccggc ctgggcgcca gtcccttgcg caccctgctg
     1561 ctggtcaatc tgatcacctg gtcggtgtgc tgcgtcttca ccctgcagtc gtcgatcgtg
     1621 ctgctggcgg ccgggtgctt cgtctggatg ctgctgggcc cctatgccga ggcggccgag
     1681 cacaccaccc tgcagaaggt ggtgccgctg gagcgccagg gccgggtgtt cggcttcgcc
     1741 cagtcggtcg agcaggccgc ctcgcccctg accgccttcc tgatcgggcc cctgacccag
     1801 ttcatcgtca tcccgttcat gaccgacggc gcgggagcgg ccgccatcgg cgactggttt
     1861 ggccggggac cggcgcgggg gatggcgctg gtgtttaccc tggccggggt ggtgggcgtg
     1921 gcggcgacgg tggtggcgtt taactcgcga tattaccggc agttgtcggc ggcctatgcg
     1981 cggggcgcct cggcgccctg accccaaatc gctccgtcat tcccgccctt gttgttcaga
     2041 ccggggacat agcggacacc tgttcggag
//
LOCUS       DAOMEO010000588         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5664_length_2069_cov_2.103277, whole genome shotgun sequence.
ACCESSION   DAOMEO010000588 DAOMEO010000000
VERSION     DAOMEO010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_2069_cov_2.103277"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLH31_12630"
     CDS             <1..255
                     /locus_tag="PLH31_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQR90076.1"
                     /translation="PTTGPAWTIDPLNGDNYGGSSTTLPAVAGYPHLTVPMGDVTGLP
                     VGLSFIGPAWSEKLLLNLGYAYEQATMKRKAPTFLKTLAP"
     gene            complement(269..430)
                     /locus_tag="PLH31_12635"
     CDS             complement(269..430)
                     /locus_tag="PLH31_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90077.1"
                     /translation="MLRSGLILILALTATAVCGCAPQKPFGPPAPYAPSAERVTPPPP
                     PIEVIRPRV"
     gene            complement(424..1056)
                     /locus_tag="PLH31_12640"
     CDS             complement(424..1056)
                     /locus_tag="PLH31_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90078.1"
                     /translation="MSKPVHIAAIALTVAAALGAAQAQAQAQAQSGRGGPGGGRPAAC
                     GEPLTGQAPDIMTYDSSTGTQVFVGGGRGGDTEVSAYNAQTRSAAALSGDLSRPGLVA
                     SCAVDGISRESYAVAPDGAGKITVSGRDERGERWAFTRADGQVEVWDPRGDNRCFKSG
                     LGIFTSSGNLGVAVANPPGALGLGVAVGDRFGGVGVGGNRNFAGVGLVGC"
     gene            complement(1053..>2069)
                     /locus_tag="PLH31_12645"
     CDS             complement(1053..>2069)
                     /locus_tag="PLH31_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90079.1"
                     /translation="DGDLLTLTSKEGGACVESLNITQSVTIVVRDYTPSRNRPILVAP
                     AGQPCVRIAPGVQYVVLEGLGIESLRAGQASCITGRATELALKGVTVRYSGEGSAMDV
                     QDSRIELQDTVFIGRTRQPVVKATGEIMAANVEVAATAIGAAFETSGDSRLRDIRVTR
                     LGDWTGSARTRNSAGFVIAGMNRQQLVKIEGLYVDGFSRGVYVGGGDETTLINPVVRD
                     ADWAVVLENANLQIRGGDLEVMRSGIYADAGGQVRSVDNRVFARPGGCEALVKGHFSG
                     ELTCRPWYEAPEVFRTPRDLARLDFDSYWPAPSVLPASATSASPQLPDDALSGGPQSG
                     GASR"
BASE COUNT          319 a          736 c          729 g          285 t
ORIGIN      
        1 ccgaccaccg gcccggcctg gaccatcgat ccgctgaacg gcgacaacta tggcggctcg
       61 tcgaccaccc tgcccgccgt ggccggctat ccgcacctga ccgtgccgat gggcgacgtc
      121 acgggcctgc cggtggggct gtcgttcatc ggcccagcct ggagcgaaaa gctgctgctg
      181 aacctgggct atgcctatga acaggcgacg atgaagcgca aggccccgac cttcctgaag
      241 accctcgcgc cctgatcagg gcgcgacctc agacccgggg gcgaatgacc tcgatcggcg
      301 gcggcggcgg ggtgacccgc tccgctgacg gcgcataggg ggccggcggt ccgaacggct
      361 tctgcggcgc gcagccgcag accgctgtcg cggtcagggc caggatcagg atcaggccgc
      421 ttctcagcat cccaccaggc ccacgccggc gaagttgcgg ttgccgccca cgccgacgcc
      481 gccgaaacgg tcgccaaccg caacgcccag gcccagggcg ccgggcgggt tggccacggc
      541 cacgcccaga ttgccgctgg acgtgaagat gcccaggccg gacttgaagc agcgattgtc
      601 gccgcgcgga tcccagacct cgacctggcc gtcggcccgc gtgaaggccc agcgttcacc
      661 gcgctcatcg cgaccgctga cggtaatctt gccggcgccg tccggcgcga ccgcatacga
      721 ctcgcggctt atgccgtcga cagcgcagga cgccaccagg cccggacggc tgagatcgcc
      781 gctcagggcc gccgccgaac gggtctgggc gttgtaggcc gagacctcgg tgtcgccgcc
      841 gcgcccgccg cccacgaaca cctgggtgcc ggtcgagctg tcataggtca tgatgtccgg
      901 cgcctgaccg gtcaggggtt cgccgcaggc cgcgggacga ccgccgccgg gaccgccacg
      961 gccgctctgg gcctgggcct gggcctgggc ctgggcagcg cccagagcgg cggcgaccgt
     1021 cagggcgatg gcggcgatat ggacgggctt gctcatcgtg aggctcctcc ggattgcggt
     1081 ccgcccgaca gagcatcgtc aggcagctgg ggcgacgccg aggtggccga ggccggcagg
     1141 acggacgggg cgggccagta cgagtcgaag tccagccggg ccagatcgcg gggcgtgcgg
     1201 aacacttccg gcgcctcgta ccaggggcgg caggtcagtt cgccgctgaa gtggcccttg
     1261 accagggcct cgcagccgcc ggggcgagcg aagacgcggt tgtcgaccga gcgcacctga
     1321 ccgccagcgt cggcatagat gcccgagcgc atgacttcca ggtcgccgcc gcggatctgc
     1381 agattggcgt tctccagcac cactgcccaa tcggcgtcac gaacgaccgg attgatcagc
     1441 gtggtctcgt cgccgccgcc tacatagacg ccgcgcgaga agccgtcgac atacaggccc
     1501 tcgatcttga ccagctgctg gcggttcatg ccagcgatca cgaagccggc cgagttgcgg
     1561 gtgcgggccg agccggtcca gtcacccagg cgggtcaccc ggatgtcgcg cagccggctg
     1621 tcgcccgagg tttcgaacgc cgcgccaatg gcggtggcgg cgacctcgac attggccgcc
     1681 ataatctcgc cggtggcctt gaccacgggc tgacgcgtgc ggccgatgaa gaccgtgtcc
     1741 tgcagctcga tgcggctgtc ctggacatcc atcgccgagc cttcgccgct gtagcggacc
     1801 gtgacaccct tcagggccag ctcggtggcg cggccggtga tgcacgaggc ctggccggcg
     1861 cgcagggact cgatgcccag cccctccagc accacatact gaacgcctgg cgcgatgcgg
     1921 acgcacggct ggccggcggg cgccaccagg atcggccgat tgcgcgacgg cgtatagtcg
     1981 cggaccacga tggtcaccga ctgggtgatg ttcagcgact cgacgcaggc cccgccctct
     2041 ttcgaggtca gggtcagcag gtcgccgtc
//
LOCUS       DAOMEO010000589         2067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5668_length_2067_cov_2.382207, whole genome shotgun sequence.
ACCESSION   DAOMEO010000589 DAOMEO010000000
VERSION     DAOMEO010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_2067_cov_2.382207"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLH31_12650"
     CDS             complement(<1..658)
                     /locus_tag="PLH31_12650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR90080.1"
                     /translation="MTNYYAATAAPFADLPPLRGDLHADVCVIGAGFTGLGAALELAE
                     RGVSVVVLEAGRVGSQASGLNGGQVHTGQRRDQAWLEKTVGLDDAMALWRLAQDARTH
                     LHATIAEHAIACDLKPGMIHARHRPGGEAEDAGHIEHMARVYGYDALSLIGPRDLADD
                     LGTDAYHGGLLDRDGGHLHPLNLALGLARAAIAGGAITHEHSRALSWTRQGGKITAVT
                     D"
     gene            complement(695..>1273)
                     /locus_tag="PLH31_12655"
     CDS             complement(695..>1273)
                     /locus_tag="PLH31_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQR90081.1"
                     /translation="MHIHQSIIDRHGQNLFSDPKTGDETPLFHSFIAGQQTYLPAIMA
                     ILAPYVNSYRRIARDSGAPVNTQWGYDNRTCGLRVPPSNAANRRLENRIPSSDSNPYL
                     AIAATLAAGYLGMVQALTPEPPVSSDASARGIELPRSLTESLKLFEACEPLVDILGPV
                     FCQAYDHVKHAEYETFMRTISPWEREFLLLNV"
     assembly_gap    1274..1283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1284..2041)
                     /locus_tag="PLH31_12660"
     CDS             complement(<1284..2041)
                     /locus_tag="PLH31_12660"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQR90082.1"
                     /translation="MKRGVATLEEAQAWFNRQNIEEIECVVPDLAGVARGKIMPVRKF
                     LGTPSMNLPLAVFYQTITGDFPEFGGVVNSVQADTDIVLTPDFATLAVVPWAQDPTAQ
                     VIHDAFHPDGRPVEESPRQVLRRVLALYDEKGWVPIVAPEIEFYLVDKNTDPDYPLKP
                     PIGRSGRPETGRQGYSISAVNEFDALFEDMYEYSERQGLEIDTLIHESGVAQMEINLR
                     HGHPLELADQVFMFKRTIREVALEHEIYATFMAKP"
BASE COUNT          322 a          639 c          727 g          369 t
ORIGIN      
        1 ggtcggtgac cgccgtgatc ttccccccct ggcgggtcca ggagagcgcc cgactgtgtt
       61 catgggtgat cgccccgccg gcgatcgccg cgcgcgccag gccaagggcc agattcaggg
      121 ggtggaggtg accgccgtcc cgatccagca ggccgccgtg ataggcgtct gtccccaggt
      181 cgtcggccag atcccggggt ccgatcagtg acagggcgtc atagccatag acgcgggcca
      241 tgtgctcgat gtggcctgca tcctcggcct cgccgcccgg gcggtggcgg gcgtggatca
      301 tgccgggttt caggtcgcag gcgatggcat gttcggcgat ggtcgcgtga agatgcgtcc
      361 gggcgtcctg cgccagtcgc cacagggcca tggcgtcgtc cagacccacc gtcttttcca
      421 gccaggcctg gtcgcgacgc tggccggtat ggacctgccc gccgttcagg cccgaagcct
      481 gcgatccgac acggccggct tcgaggacca ccacggaaac gccgcgctcg gccagttcaa
      541 gcgccgcgcc caggccggtg aagcccgcgc cgatcacgca aacgtcggcg tgcaggtcgc
      601 ccctcagtgg cggcaggtcg gcgaagggcg cggcggtcgc ggcgtaatag ttcgtcatcc
      661 cccttccccc tctatgggga agggtttgag cggactaaac attcagcagc aggaactccc
      721 gctcccaggg gctgatcgtg cgcatgaagg tctcgtactc ggcgtgtttc acatggtcgt
      781 aggcctggca gaacaccggt ccaaggatgt cgaccagcgg ctcgcaggcc tcgaacagtt
      841 tcagggattc ggtcaggctg cggggcagtt cgatgccgcg agcgctggcg tcgctactga
      901 ccgggggctc gggcgtgagc gcctgaacca tgcccagata gcccgctgca agggtggcgg
      961 cgatggccag ataggggttg gagtcggacg atgggatgcg attttccagc cggcggttgg
     1021 cggcgttgga cgggggaacg cgcagaccgc aggtgcgatt gtcatagccc cactgggtgt
     1081 tgaccggcgc gcccgagtcc ctggcgatcc ggcggtacga gttcacatag ggcgccagta
     1141 tggccatgat cgccggcagg taggtctgct gccccgcgat gaagctgtgg aacagcggcg
     1201 tctcgtctcc ggtcttggga tcggaaaaca gattctggcc gtgcctgtcg atgatcgact
     1261 ggtggatatg catnnnnnnn nnnggcttgg ccatgaaggt ggcgtagatc tcgtgctcca
     1321 gcgccacctc gcggatggtg cgcttgaaca tgaacacctg gtcggccagt tccagcggat
     1381 ggccgtggcg caggttgatc tccatctggg ccacgccgct ctcgtggatc agggtgtcga
     1441 tctccaggcc ctggcgctcg gaatattcgt acatgtcctc gaagagggcg tcgaactcgt
     1501 tgaccgcgct gatcgaatag ccttggcgcc cggtctcggg ccggcccgag cggccgatgg
     1561 ggggcttcag cggatagtcg gggtcggtgt tcttgtcgac gaggtagaac tcgatctcgg
     1621 gcgcaacgat ggggacccag cccttttcgt catacagggc cagtacgcgg cgcagcacct
     1681 ggcgcgggct ttcctcgacg gggcggccat cgggatggaa ggcgtcatgg atgacctggg
     1741 cggtgggatc ctgggcccag ggcacgacag ccagggtggc gaaatcgggc gtcaggacga
     1801 tatcagtgtc ggcctgcacc gaattgacca cccccccgaa ctcgggaaag tcgccggtga
     1861 tggtctggta gaacaccgcc agcggcaggt tcatcgacgg ggtcccgagg aacttgcgca
     1921 ccggcatgat cttgccgcgc gccacgccgg ccaggtcggg cacgacgcac tcgatctcct
     1981 cgatgttctg ccggttgaac caggcctggg cttcctcaag cgtggcgacg ccgcgcttca
     2041 cccgctggtc cggcttggtc ttcttgt
//
LOCUS       DAOMEO010000590         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5685_length_2063_cov_2.645418, whole genome shotgun sequence.
ACCESSION   DAOMEO010000590 DAOMEO010000000
VERSION     DAOMEO010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_2063_cov_2.645418"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..702)
                     /locus_tag="PLH31_12665"
     CDS             complement(52..702)
                     /locus_tag="PLH31_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HQR90083.1"
                     /translation="MRLRLAPGLHGAMIEGDLVLLDVAADAYFCLPAAARDLVLSGSE
                     LEVTPAALAEGLCEVGLALPLVAGLGDARPTPPPPTRTARRLIETLDAASRTRPCHGL
                     ALTKAVAAAIAGRRLAFEALLPAAVPFAVQPPTPALLSDLAVYRRIVPWLPIDGACLF
                     RSHMLRAYLTTLGHGVSWVFGVRTWPFSAHCWLQAGDVALDDEAERLTAYAPILVL"
     gene            complement(1207..1821)
                     /locus_tag="PLH31_12670"
     CDS             complement(1207..1821)
                     /locus_tag="PLH31_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR90084.1"
                     /translation="MGRDTDPFDRTMEALRRRILAVAPLQGAALPINGVALEFGVSQT
                     PVREALSRLAGEGLIERTPAGYRGVIHDPASLADLYALAGVLALALLRSVEGASSQAA
                     APPDLAPDDKTPSAALDIIAVIAARAGNRAVARAYGRAAAQLAPFREAERQVLSDVEA
                     ERVRLVQAFVAGDPGLTRQLRRYHDRRAARSGRILAAALGLLQP"
BASE COUNT          344 a          702 c          752 g          265 t
ORIGIN      
        1 ggaacagccg ggacggctcg cccggcgggc aggtgaggat cagatagccc atcagagcac
       61 caggatcggc gcataggccg tcaggcgctc ggcctcatcg tcgagggcga cgtcgccggc
      121 ctgcagccag cagtgagcgg agaacggcca tgtgcgcacc ccgaacaccc agctgacgcc
      181 gtggcccagg gtcgtcagat aggcccgcag catgtggctg cggaacaagc aggcgccgtc
      241 gatcggcagc cacgggacga tccggcgata gaccgccagg tcgctcagca gcgccggggt
      301 cggcggctgg accgcgaacg ggacggccgc cggcaacaga gcttcgaagg caagccgccg
      361 gccggcgatg gcggccgcca cggccttggt cagggccagc ccatgacagg gccgtgtgcg
      421 ggaagccgcg tccagggttt cgatcagccg gcgggccgtt cgggttggcg gtggcggcgt
      481 cggccgggcg tcgccaagac cggcgaccag cggcagggcc aggcccacct cgcacagccc
      541 ctcggccagg gccgccggtg tgacctcgag ctccgaaccg ctcagcacaa ggtcgcgcgc
      601 cgccgccggc agacagaaat aggcgtcggc cgcgacgtcg agcaggacga gatcgccctc
      661 gatcatcgcg ccatggagtc ctggcgccag gcgcagtcgc atcgccccct ccggtcacac
      721 gctgaaatgg atggaatgaa agtcgccgtc ggcgggtctc caccgccgac ggcgaggacc
      781 gtctagagcg ggtcgtattt gaggacgaaa ttgtcctcca gcagcatcca gccgccgccc
      841 gtctgggtca gggccttggc cgagccaagg cggatcatgc tgacgtcgcg catgaaaccc
      901 tcctttgtgt ggaagacgcg gccgatcagc gaccgcgaga accggcgatc ccaagaggga
      961 ccgccggggc gaaccgccga cgagcgcgag cgcggtcgtc ggcggagggg cgggtcaggc
     1021 ttggtcgtag cggaggatga agttgtcctc gtagtcgacg ccgccgccac cggtctgggt
     1081 caggaccttg gccgagccca ggtggatcag ttggacacgc atcgcacatc tccttgcttg
     1141 atcagccgca tcatcgcggc cccccaatca ggcgacgcta gagacgtata ttcaaaccta
     1201 tattctctat ggttgcagga gaccgagagc cgccgccagg atccgaccac tgcgcgcggc
     1261 gcggcgatcg tggtagcgcc gaagctgccg ggtcagaccg ggatcaccgg cgacgaaggc
     1321 ctggaccagc cggacgcgct cggcttcgac atcggacaga acctggcgct cggcttcgcg
     1381 gaacggggcc agttgggcgg cggcgcggcc gtaggcccgg gcgaccgcgc gattgccggc
     1441 gcgggcggcg atgacggcga tgatgtcgag cgccgccgac ggcgtcttgt cgtcgggcgc
     1501 gaggtccggc ggcgcggccg cctggctcga cgccccttcg accgagcgca gcagagccag
     1561 ggccaggacc ccggccaggg catagagatc ggccaggctg gccgggtcgt ggatcacacc
     1621 gcgatagccg gcgggtgtgc gttcgatcag cccctccccc gccaggcgcg agagcgcctc
     1681 tcgcaccgga gtctggctga cgccaaactc cagggccacg ccgttgatcg gcaaggccgc
     1741 gccctgcagc ggagccaccg ccaggatccg ccgacgaagg gcctccatgg ttcggtcgaa
     1801 tggatcggtg tcgcggccca cgcctgtctc cctgcgggga aggagtgtcg aggtcgcgcc
     1861 ccggcccgtc gaggcgcgag ccccgcgcct ggcgatcgcc gcggccgcgc aaggtcgatg
     1921 cgcaacgatc gcggacgccg ggtacagaag cctgctggcc gacgccgcga tcactgccgg
     1981 ttttgtggca gaggccggta aaagacttcc ggccaggaca ttgcgccggg ccgccgggca
     2041 tccagtcgca tcgatgcgcg ttg
//
LOCUS       DAOMEO010000591         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5687_length_2062_cov_2.609865, whole genome shotgun sequence.
ACCESSION   DAOMEO010000591 DAOMEO010000000
VERSION     DAOMEO010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_2062_cov_2.609865"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLH31_12675"
     CDS             <1..390
                     /locus_tag="PLH31_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90085.1"
                     /translation="AAHAGTDTHIETCRRWLAIDAEQRRLQLEWGTLEGWLMTKRNWY
                     QLSPDQQAAIPESARLAEIDARLDLLEVEGHALLKTMRPKPAKSIEAVVANLSVAARL
                     IFVEDHPEAHGLITRAVRDLAAFSGGK"
     gene            497..838
                     /locus_tag="PLH31_12680"
     CDS             497..838
                     /locus_tag="PLH31_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HQR90086.1"
                     /translation="MARFRKPWLLVVSQGWRWRHPDLWHGRVFDPHNAQQVMSYAVLR
                     LRRETRDVFLLNHIEALDYALIARHLGLSVADVQARLADALCEISRTIDLIERIRPTP
                     INLSHAEHPDV"
     gene            831..1325
                     /locus_tag="PLH31_12685"
     CDS             831..1325
                     /locus_tag="PLH31_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90087.1"
                     /translation="MSDDKAPFAVSRRVVIGAASAAPMVAGAGAAAPSSEAVTRCAEW
                     LALDAEIDRLGLRWSDLEAVLVRQKRWERMTQEERGELAPSEEMDAIDEQLKPLFVQR
                     EAWLEALPKVHARDLHGVVAKLDVALQVMGHQQGDGYDLFRATMDELRTVRCPHCGSP
                     VCKS"
     gene            complement(1560..1802)
                     /locus_tag="PLH31_12690"
     CDS             complement(1560..1802)
                     /locus_tag="PLH31_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HQR90088.1"
                     /translation="MDTRAWVMQRRERTHHLIELGGLVQKAGLVELTQDDRATLYGAF
                     LTLAMMLKGENREHILALWRRSGKRAFEAEAADGQA"
     gene            complement(1819..>2062)
                     /locus_tag="PLH31_12695"
     CDS             complement(1819..>2062)
                     /locus_tag="PLH31_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HQR90089.1"
                     /translation="KERKVRQLGELVIATGADNLDVETLAGGLLGLVETKDAAGKAEW
                     RKRGAGFFRGGKADPAPSAGGNPLRPAPNHGGASQA"
BASE COUNT          321 a          725 c          663 g          353 t
ORIGIN      
        1 gcggcgcacg ccggaactga tacccacatc gaaacctgcc gccgctggct agccatcgac
       61 gccgaacagc ggcggctcca gttagagtgg gggacgctcg aaggctggct gatgaccaag
      121 cgcaattggt atcagctgtc accggaccag caggcggcga tccccgaaag cgccaggctt
      181 gccgagatcg acgcacggct cgacctgttg gaggttgagg ggcacgccct cctgaagacc
      241 atgcggccga agcccgcaaa gagcatcgag gcggtcgtcg ccaacctgtc ggtggcggcg
      301 cgcctgatct tcgtggaaga ccatcccgaa gcccacggcc tgatcacccg cgcggtccgc
      361 gacctcgccg ccttttccgg tggcaagtga ccttccgggt tccccggcaa agcgacggct
      421 ccgaccgccc gctggcggcg acgttccacc gcaatgccgc ccggcgcgaa gcttcaaatc
      481 atgaaggtgc cgcgtgatgg cgcgcttccg aaagccttgg ctgcttgtcg ttagccaggg
      541 gtggcggtgg cgacatcctg atctctggca cggtcgtgtc ttcgatccgc acaacgccca
      601 gcaggtcatg tcctatgccg tgctccgcct tcggcgcgag acgcgagatg tcttcctgct
      661 caaccacatt gaggcgctcg actacgcgct gatcgctcgc cacctggggc tttccgttgc
      721 tgacgtccag gcgcgcctgg ccgatgcgct ttgcgagatt tcccgcacga tcgatctgat
      781 cgagcgtatc cgccccacgc ccatcaacct gtcccatgcg gagcatcccg atgtctgacg
      841 acaaggcgcc cttcgccgtt tcacgccgcg tcgtgatcgg cgcggccagc gccgcgccga
      901 tggtcgctgg cgccggcgcg gcggccccat cgagcgaggc ggtaacgcgc tgcgccgagt
      961 ggctggcgct cgacgcggag atcgaccggc tgggtctgcg ctggtcggat ctggaggcag
     1021 tcctggtgcg tcagaagcga tgggagcgca tgacgcaaga ggagcgtggg gaattggcgc
     1081 cctccgaaga gatggacgcc atcgacgagc agttgaagcc gctgttcgtg cagcgcgagg
     1141 cctggctcga agccctgccc aaggttcatg cgcgtgacct gcacggcgtc gtcgccaagc
     1201 tggatgtggc gctgcaggtc atgggccacc agcagggcga cggctacgac ctcttcaggg
     1261 cgacgatgga cgagctgcgc accgtgcgct gtccgcactg cgggtcaccg gtgtgcaaga
     1321 gctaaccgtg cctccacccg caaccgcctt gcccatccca atcggcggga gccaggaccg
     1381 gaacggtcgg tgctcgccct ggagcgcttg gagagcctat gccgggcggg atggtgcggg
     1441 tcaagggcgg cttcgccgtc gctccgcgat gcgcccgcag ggcgcaccct tgacgcgcgc
     1501 cagcccgtcc ggccctcgtt ccaccaagcg atccacggcg gtttgtctcc agacgggggt
     1561 caggcctgac cgtcggcggc ctccgcctcg aacgcgcgct ttccgcttcg ccgccacagc
     1621 gccaagatat gttcgcggtt ctcgcccttc agcatcatcg ccaaggtcag gaacgcgcca
     1681 tagagcgtgg cgcgatcatc ctgggttagt tcgaccaggc ccgccttctg caccaggccg
     1741 cccagttcga tcaggtgatg ggttcgctcg cgccgctgca tcacccaggc ccgcgtgtcc
     1801 atcctggccc ttcgtgcttc aagcctgtga cgcgccgccg tggttcggcg cagggcgtag
     1861 tggattaccg ccagcgcttg gcgcagggtc ggccttcccg ccacgaaaga accctgcccc
     1921 tcgtttgcgc cactccgcct tgccggccgc gtccttcgtc tcgaccagac ccagcaatcc
     1981 gcccgccagc gtctcgacat ccagattgtc agccccggtg gcgataacca gctcgccaag
     2041 ttgcctgacc ttccgctcct tt
//
LOCUS       DAOMEO010000592         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5700_length_2058_cov_2.476286, whole genome shotgun sequence.
ACCESSION   DAOMEO010000592 DAOMEO010000000
VERSION     DAOMEO010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5700_length_2058_cov_2.476286"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..946
                     /locus_tag="PLH31_12700"
     CDS             233..946
                     /locus_tag="PLH31_12700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR90090.1"
                     /translation="MKISGARTEPFAAIRKRALARAGAQVAVRETQSPDSAAFLGLTE
                     ADLTPQVQEALSTLMSEIDDLRAEVSRLKLRLNEAQFQADQDVLTPVLNRRAFLREMK
                     RVAAFAQRYGSPASVVFFDLDGFKSVNDRFGHAAGDAALQAVARRLLANVRESDVVGR
                     MGGDEFAVLLAQADKDTALLKAASLAAAVQDTPVEFGEWSAPLHISWGVREIAPGSDP
                     EVALAEADAAMFVRKRRQA"
     gene            1075..1437
                     /locus_tag="PLH31_12705"
     CDS             1075..1437
                     /locus_tag="PLH31_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90091.1"
                     /translation="MQRILIAEDDPILAMIYEITLSEAGFDVLVCRDGVEALDALDDF
                     APDLVITDFFMPRMTGGELIRAVRERRGGLTCTLLASAVDPARLREDEWADARLEKPI
                     TPARLLSGVRALEACQAH"
     gene            1515..2054
                     /locus_tag="PLH31_12710"
     CDS             1515..2054
                     /locus_tag="PLH31_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQR90092.1"
                     /translation="MTHECAKSIVRRMHDPKFASHYFVGQGLDVGGAPDPLSSFKPFF
                     PLMTGCDTWDLPQGDGQVLETVADETYDFLHSSHCLEHLVDPMVSLRNWLRVLKPGGT
                     LSSLGVYSSERSLNVVDLLRHIGPQAEIQKIECLNSTFYHGMGRFDQTRLPTTECAIE
                     FVVRKRPESEVAAKGRLPG"
BASE COUNT          341 a          681 c          687 g          349 t
ORIGIN      
        1 ggcataggcg tcgaggtccc cttggcggcg gcgggcctga aagccgcccc gtatgcgcga
       61 cacttgcatt tgcgcaagcc caaaacgccc tgcgcgcctt cgaaacgggc gccccttggc
      121 ctgaacgcgg cgtgcgacgc caaggccctg cgactaaaac gcgccccgat tgcgccgaac
      181 gccgttcccg ttaacaattg accaagctgc gcaacaagcg attcccgacc tgatgaagat
      241 ttccggcgcc cggaccgaac cttttgccgc catccgcaag cgggccctgg cccgtgcggg
      301 ggcccaggtc gccgtgcgtg agacgcaaag ccccgatagc gcggcgtttc tgggtctgac
      361 agaggccgac ctgacgcccc aggtgcagga agcgctgtcg accctgatga gcgagatcga
      421 cgacctgcgg gccgaggtct cgcggctgaa gctgcggctg aacgaagccc agttccaggc
      481 cgaccaggat gtgctgaccc ctgtgctgaa ccgccgcgcc ttcctgcgcg agatgaagcg
      541 cgtggccgcc ttcgcccagc gctatggctc gccggccagc gtggtgttct tcgatctgga
      601 cggcttcaag agcgtcaatg atcgtttcgg ccatgcggcg ggcgacgcgg ccttgcaggc
      661 tgtggcccgc cgtctgctgg ccaatgtgcg cgaaagcgac gtggtcggac ggatgggcgg
      721 cgacgagttc gccgtgctgc tggcccaggc cgacaaggac actgcgctgc tcaaggccgc
      781 cagcctggcc gccgcggttc aggacacgcc ggtcgagttt ggcgaatggt cggcgccgct
      841 gcacatttcg tggggcgtgc gcgagatcgc gccgggatcc gaccccgaag tggccctggc
      901 cgaggctgac gcggcgatgt tcgtgcgcaa gcgccggcaa gcctagcccg ccagcacacc
      961 tctgggtctt gcgacgtttt gaccggggac tcaggccggc gccgcgcgct accaagttaa
     1021 tcgttggtta agcaaaggcg ttctgactgt gggtcggagc gtgtggggcg cgagatgcag
     1081 cgaattctga tcgccgagga cgatccgatc ctggcgatga tctacgagat cacgctgagc
     1141 gaagccggct tcgacgtcct ggtgtgccgg gacggcgtcg aagcattgga cgcgctggac
     1201 gacttcgcgc ccgatctggt gatcaccgac ttcttcatgc cgcgcatgac cggcggggaa
     1261 ctgatccgcg ccgttcgcga gcgtcgcggc ggcctgacct gcaccctgct ggcctccgcg
     1321 gtcgatccgg cccggctgcg tgaggacgaa tgggccgacg cccgcctcga aaaacccatc
     1381 accccggctc gcctgctcag cggcgtgcgg gcccttgagg cctgccaagc tcactgaccg
     1441 gggcgcactg accgggccgc actgacaaac ccggcaaatt gggcgatgaa cggcgttcgt
     1501 cacaggggtc ccgcatgaca cacgagtgcg ccaagtccat cgttcgccgg atgcacgacc
     1561 ccaagttcgc cagccactat ttcgtgggtc agggccttga tgtgggcggc gcgcccgatc
     1621 cgctgtccag cttcaagccg ttctttccgc tgatgaccgg ctgcgacacc tgggacctgc
     1681 cgcagggcga cggccaggtc ctggagacgg tggccgacga gacctatgat ttcctgcatt
     1741 ccagccactg tttggaacat ctggtcgatc cgatggtcag cctgcgcaac tggttgcgcg
     1801 tgctcaagcc cggcggcacc ttgtccagcc tgggcgtgta ttcgagcgag cgctcgctga
     1861 atgtggtcga cctgctgcgc cacatcggcc cgcaggccga gatccagaag atcgagtgcc
     1921 ttaacagcac attctatcac ggcatgggtc gctttgatca gacgcggctg ccgaccaccg
     1981 aatgcgccat cgagttcgtg gtgcgcaagc ggccggagtc ggaagtggcc gccaagggcc
     2041 ggctacccgg ctagatct
//
LOCUS       DAOMEO010000593         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5705_length_2057_cov_2.841658, whole genome shotgun sequence.
ACCESSION   DAOMEO010000593 DAOMEO010000000
VERSION     DAOMEO010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_2057_cov_2.841658"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLH31_12715"
     CDS             <1..238
                     /locus_tag="PLH31_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQR90093.1"
                     /translation="TGGGVVALVGTSDGKAAVTVAVTADLVDRFSAADLARAAVLAMG
                     GQGAGGKADFAQGGAPDATKAQEGLDAIKAALAG"
     gene            complement(235..612)
                     /locus_tag="PLH31_12720"
     CDS             complement(235..612)
                     /locus_tag="PLH31_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR90094.1"
                     /translation="MISYITIGASDIDRAGAFYDAALAALGSARQWRQGDWIGYGPDP
                     EHATIMICPPQNGQPARAGNGIMVGLKAETPEQVDAFHAAALANGGTCEGPPGPRPAY
                     GEGYYLAYLRDPDGNKLSAFLKR"
     gene            complement(676..1620)
                     /locus_tag="PLH31_12725"
     CDS             complement(676..1620)
                     /locus_tag="PLH31_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQR90095.1"
                     /translation="MAGLVLDVLSRVLPFFLLVGAGVALVRLRLIDAPMAAGLSAYVF
                     WIGFPALLIHSLSRIGTPGPALVWGLAAFAGAALLVMAGLLGLGRLLGWTREEQAGAA
                     LAGALGNSAFLGLPITAAVLGPEAARLAAAVLAVDFVIVAAVGIAALGWAGGRSIPGS
                     ALRALVNPVVAAALIGLALALTGVALPAVLDRAVGAAAASGSPVALVALGAALALRRT
                     GDEAVPRAGPVLAATVAKLLVLPVLTWLAVGLTDAPAAFRIGATLLAATPTAVNVFIQ
                     TRTLGVFARGAARTVALTTAAAVITLSVAAILLTRGVS"
     gene            complement(1644..>2057)
                     /locus_tag="PLH31_12730"
     CDS             complement(1644..>2057)
                     /locus_tag="PLH31_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90096.1"
                     /translation="VRRSLTGQLQEAAPVAAAEPPSIWRDPMGAMRRAIEPIAPPEPK
                     VDRYLTIAGISALTRGYAPGAAPAGAARPETVGGKVKAVVSGPYPTLAYWDPNRVRVA
                     MKRPGDARKVTVFTFQRRALFTWKLVHIKLPRDER"
BASE COUNT          313 a          768 c          746 g          230 t
ORIGIN      
        1 gaccggcggc ggcgtcgtcg ccctggtcgg aaccagcgac ggcaaggccg ccgtcaccgt
       61 ggccgtcacc gccgacctgg tcgacaggtt cagcgccgcc gacctggcca gggccgccgt
      121 gctggccatg ggcggacagg gcgcaggcgg caaggccgac ttcgcccagg gcggcgcgcc
      181 tgatgcgacc aaggcccagg agggcctgga cgcgatcaag gcggcgctgg ccggctagcg
      241 cttcaggaac gccgacagtt tgttgccgtc cgggtcgcgc agataggcca gatagtagcc
      301 ctcgccatag gcggggcgcg ggcccggcgg tccctcgcag gttccgccat tggccagggc
      361 ggcggcgtgg aaggcatcga cctgttcggg cgtttccgcc ttaaggccga ccatgatgcc
      421 gttgccggcc ctggcgggct gaccgttctg gggcgggcag atcatgatgg tagcgtgctc
      481 gggatccggg ccatagccga tccagtcgcc ctgccgccac tgccgcgcgc tgcccagggc
      541 ggccagagcc gcgtcataga aggctcccgc ccggtcgata tcgctggccc cgatggtgat
      601 gtagctgatc atgtcgttct ccttctccac acagcctagc caggcgaaca ttgttcgtcg
      661 agaacgaacc gggaactagc tgacaccccg tgtcagcagt atcgccgcga ccgacagggt
      721 gatgaccgcc gccgccgtgg tcagggccac cgtccttgcc gcgccgcggg cgaagacgcc
      781 cagggtgcgg gtctggatga agacgttgac cgccgtcggc gtggcggcca gcagggtggc
      841 cccgatccgg aaggcggccg gcgcgtcggt caggcccacg gccagccagg tcaggaccgg
      901 caggaccagc agcttggcca ccgtcgccgc caggaccggt ccggcgcggg ggacggcctc
      961 gtcgcccgtc cggcgcaacg ccagggccgc gcccagcgcc accagcgcca ccggactgcc
     1021 agaggccgcc gcggcgccga cggcgcggtc cagcacggcc ggcagagcca cgccggtcag
     1081 cgccagggcc aggccgatca gcgcggcggc caccaccgga ttgaccagcg cccgcagggc
     1141 cgagcccggg atcgaccgcc ccccggccca gcccagtgcg gcgatcccca ccgccgccac
     1201 gatcacgaag tcgaccgcca ggacggccgc ggccagacgc gccgcctcgg ggcccagaac
     1261 ggcggcggtg atcggcaggc ccaggaaggc gctgttgccc agagcccccg ccagggcggc
     1321 gccggcctgt tcctcgcggg tccagcccag caggcggccc agacccagca gcccggccat
     1381 caccagcagc gccgccccgg cgaaggccgc cagcccccag acgagggccg gtcccggcgt
     1441 gccgatccgc gacagcgaat ggatcagcag ggccggaaag ccgatccaga acacataggc
     1501 cgacagcccc gccgccatcg gcgcatcgat caggcgcagg cggaccagcg ccacgcccgc
     1561 cccgaccagc aggaaaaacg gcaggacgcg cgacaggacg tccaggacga gacccgccat
     1621 ggcgaagacc ggcggtcctg gccctagcgc tcgtctctgg gcagcttgat gtggaccagc
     1681 ttccaggtga acagcgcgcg gcgctggaag gtgaacaccg tcaccttcct ggcgtcgccc
     1741 ggccgcttca tcgccacccg cacgcggttg ggatcccagt aggcgagggt ggggtagggg
     1801 cccgacacca cggccttgac cttgccgccc acggtttcgg gccgggccgc gcccgccggc
     1861 gccgcgccgg gggcatagcc ccgggtcagg gccgagatgc cggcgatggt cagatagcgg
     1921 tcgaccttgg gctcgggcgg agcgatcggc tcgatggccc gccgcatggc ccccatcggg
     1981 tcgcgccaga tcgacggcgg ctcggccgcc gccacgggcg cggcctcctg cagctggccg
     2041 gtcaggctgc ggcgcac
//
LOCUS       DAOMEO010000594         2057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5707_length_2057_cov_2.047952, whole genome shotgun sequence.
ACCESSION   DAOMEO010000594 DAOMEO010000000
VERSION     DAOMEO010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_2057_cov_2.047952"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1212)
                     /locus_tag="PLH31_12735"
     CDS             complement(271..1212)
                     /locus_tag="PLH31_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR90097.1"
                     /translation="MGAQQETIVRDMLSAWGDGNRPPDIERVVSFFAEDARWTLYMPD
                     GPTVVGRAAIRAELTRQATYVEQVETEITLIASSEDMVMAERRDRFVRNGRSIDRSIA
                     GAFEIDTEGRIAAWRDYFDGLDFAGQAAVSPDLMSGLEGTPTSAGLDTRPPIPEATDG
                     SGLPVALHPPQGVEQAFIEAFCDAWGDGTATSRPDVDRIIAMMAPDAEWRLWVPGGPL
                     VQGRDALRAEINRQIAYSGHNKCNTVHAVSNPRVVIQERSDWAVLLGRPCPHQMIAIY
                     ELDDDGLIIRWREYINMADLDRKRGVKAEVAHVQIDH"
     gene            complement(1216..>2057)
                     /locus_tag="PLH31_12740"
     CDS             complement(1216..>2057)
                     /locus_tag="PLH31_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90098.1"
                     /translation="MTRLTGKTAVITGAGSGIGRASALKFAAEGAAVAVTDILGETAE
                     AVAEEIRAAGGRAVGFAVDVADDLALKDMIHRTIAELGKLDILFNNAVNTAAGKRKRD
                     VDFLEFDPAVFHEIVQVNVLGGVLASKYALPHMLERGNGCILFTSSTSSLGGDVAQFS
                     YGASKAMVNWYVKTIATNYGKQGIRCNAVIPGVIETPAMKAWASAEMHAAFMKLQNAP
                     RLGQPEDIANLAAYLASDEAAFMNGGLYKCDGGMTAPLPFAQLQLDYLIPRHKPGAAP
                     GET"
BASE COUNT          349 a          635 c          665 g          408 t
ORIGIN      
        1 gataagcgca agcgcgcttg ctcccgcaag cagaaggtgc ctagccatgt ttccccctaa
       61 ggaaatgatg tggaccgact caggacagtc ttttgccgtc ctttgatgtg tgagccggtt
      121 ttgaggtcag tgatttgtgc gtgaactgtc gcgtctacct cgcgtaaggc cgatgcaggc
      181 gcaaacatct agcgaagagt tgtcggatcc tgttttgata ctttggatct gacacgagat
      241 tggtcttctg aaatttgtaa gtcaagtgac ttaatggtcg atttgaacgt gagcgacctc
      301 agccttgacg ccgcgttttc ggtcgaggtc ggccatgttg atgtactcgc gccagcgaat
      361 gatcagaccg tcgtcgtcca gttcgtagat cgcgatcatc tggtgcggac atggacgtcc
      421 cagcagcacg gcccaatcgg atcgctcctg gatgacgacg cgcgggttcg acaccgcatg
      481 gacggtgttg cacttgttgt gacccgaata ggcgatctgc cggttgattt cggcgcgcag
      541 ggcgtcgcgg ccctggacca acggtccgcc cggaacccac agacgccatt cggcgtcggg
      601 agccatcatg gcgatgatgc ggtccacgtc gggtctgctt gtggccgtgc cgtcgcccca
      661 ggcgtcgcag aaggcttcga tgaatgcctg ctcgacgcct tgcggcgggt gcagggcgac
      721 cggcaagccg gacccatcgg tggcttccgg aattggcggg cgagtatcca ggccggcgct
      781 tgtcggggtc ccttcgaggc cggacatgag atcaggcgaa acggccgcct gaccggcgaa
      841 atccagtccg tcgaaatagt cgcgccaggc cgcgatccgg ccctcggtgt cgatctcgaa
      901 ggcccctgcg atcgatcggt cgatcgatct tccattgcgg acgaagcggt cgcgtcgctc
      961 ggccatgacc atgtcctcgc tggaggcgat cagtgtgatc tccgtttcga cctgttcgac
     1021 ataggtcgcc tgccgcgtca gctcggcccg gatcgcggcc cgtcccacga cggtcggccc
     1081 gtccggcatg tagagcgtcc agcgggcgtc ttcggcgaag aacgagacga cgcgttcgat
     1141 gtccggtggg cgatttccat cgccccaggc gctcagcatg tcgcggacga tggtttcctg
     1201 ttgcgcgccc atcggtcagg tttccccggg ggcggcgccc ggcttgtgcc gggggatcag
     1261 ataatctagc tgaagctggg cgaagggcag gggcgcggtc atgccgccat cgcacttgta
     1321 aagcccgcca ttcatgaacg cggcttcgtc cgaggccaga taggcggcca ggttggcgat
     1381 atcctcgggc tgacccaaac gcggcgcgtt ctgcagtttc atgaaggccg catgcatctc
     1441 ggcgctggcc cacgccttca tcgccggggt ctcgatcacc ccggggatga ccgcgttgca
     1501 acggattccc tgcttgccgt agttggtggc gatggttttg acgtaccagt tcaccatcgc
     1561 cttggacgcg ccataggaga actgcgcgac atcgcccccc aacgaactgg tggaggaggt
     1621 gaacaggatg cagccattgc cgcgctccag catgtggggc agggcatact tggaagccag
     1681 gacgccgccc agcacattga cctggacgat ctcgtggaag accgctggat cgaactctag
     1741 gaaatcgacg tctcgcttgc gtttgcccgc cgccgtgttg acggcgttgt tgaacagaat
     1801 gtccaacttg cccaactcgg cgatcgtgcg gtggatcatg tccttcaggg ccagatcgtc
     1861 ggcgacgtcg acggcgaagc ccacggcccg gcccccggcg gcgcggatct cctcggcgac
     1921 ggcctcggcg gtttcaccca ggatatcggt caccgccacc gcggcgccct cggccgcgaa
     1981 cttcagcgcc gaggcgcggc cgatccccga gccggccccc gtgataacgg ccgtcttgcc
     2041 ggtcaagcgt gtcatgc
//
LOCUS       DAOMEO010000595         2053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5713_length_2053_cov_2.869870, whole genome shotgun sequence.
ACCESSION   DAOMEO010000595 DAOMEO010000000
VERSION     DAOMEO010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_2053_cov_2.869870"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="trbG"
                     /locus_tag="PLH31_12745"
     CDS             <1..650
                     /gene="trbG"
                     /locus_tag="PLH31_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HQR90099.1"
                     /translation="SPGALYQVYVAPGQVTDIALEPGEQLTGSGPVAAGDTVRWIIGD
                     TESGDGDSRRVHLLVKPTRPAIETNLVVNTDRRTYLIELRAHERPYIPSVAWFYPESR
                     GKSIRAVPPTPIVPEMVQRRYRYAIDGDRPPWRPVNAYDDGRKVYIEFSPGIVQGEMP
                     PLFIVGPDGKPEIVNYRTYGNVLIVDRLFAAAELRLGGENQQKVRIVRADGRPQR"
     gene            728..1816
                     /locus_tag="PLH31_12750"
     CDS             728..1816
                     /locus_tag="PLH31_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HQR90100.1"
                     /translation="MRLSRGVLIGGTALASILICGVVLWSLRGDRLRGAAPQDLYTTD
                     RPRMADGLAGLPRDYTGVPRDIPPLGPPLPGDLGRPIVAAQGQSGPPSPDAEQQRRAQ
                     EMEAARVSRLFAPGNRQQQPVGAGSSPSQASTSGTVPSGDEAAIQNAQDRKLAFTNAP
                     VDRRTTSPDRLARPASPNVLQAGTVIPAALVTGLRSDLPGQITAQVTENVYDTPTGGS
                     LLIPQGARLIGVYDSQVSFGQSRLLLVWTRLILPNGYSIVLERQPGADAAGQAGLEDG
                     VDHHWGSLFKAALLSTILAVGTELGSDQNDSDIVRALRRGTGDTLNQAGQQVVRRNLN
                     IQPTLTIRPGYPVRVIVTRDLVLRPYQG"
     gene            1827..>2053
                     /locus_tag="PLH31_12755"
     CDS             1827..>2053
                     /locus_tag="PLH31_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HQR90101.1"
                     /translation="MSKLRLGAILDEKPVKLTVDLPATVHRELLAYAEILARETGQAV
                     DPAKLVAPMLGRFMATDRAFARERRIARRVES"
BASE COUNT          345 a          706 c          652 g          350 t
ORIGIN      
        1 acagcccggg cgccctctat caggtctacg ttgcgccggg acaggtcacc gacatcgccc
       61 tcgagccggg cgagcagctc accggctccg ggccggttgc ggccggcgac accgtgcgct
      121 ggatcatcgg cgacaccgag agtggcgacg gtgatagccg acgcgtccac ctcctggtta
      181 agccgacccg gcctgcgatc gagaccaacc tcgtggtcaa caccgaccgg cgcacctatc
      241 tgatcgagct ccgcgcgcac gagcggccct acattccctc cgtggcctgg ttctatccgg
      301 agagccgagg caagagcatt cgcgccgtgc cgccaacgcc aatcgtcccg gagatggtcc
      361 agcggcgtta ccggtacgca atcgacggag acaggccgcc ctggcggccc gtcaacgcct
      421 atgacgacgg ccgcaaggtc tacatcgagt tctcccccgg catcgttcag ggtgagatgc
      481 cgcctctttt catcgtcggt ccggacggca agcccgagat cgtcaactac cgcacttatg
      541 gaaacgtgct gatcgtcgac cggctgtttg ccgcggccga gctgcggcta ggcggcgaga
      601 atcagcaaaa agtccggatt gtccgggctg acggcaggcc gcagcgatga cggatcaatc
      661 agcacctgac ccccaaccca cgacgggcgc tggtcccttc aggctgcggt cggaggggcc
      721 gcgcgtcatg cgcctgtcgc gcggtgttct catcggcggc accgcgctgg cctcaattct
      781 catctgcggc gtcgtgctgt ggtccttgcg gggtgatcgg ctgcgcgggg ccgcacccca
      841 ggatctctac accaccgacc ggccgcgtat ggctgatggc cttgccgggc tgccgcgcga
      901 ctacacagga gtgcctcgcg acatcccgcc gcttgggccg ccgctgcccg gcgacctcgg
      961 ccggccgatc gtggcggccc aaggccaatc cggcccgcct tcgcccgatg ccgagcagca
     1021 gcgccgtgcg caggagatgg aagcagcccg cgtcagccga ctgtttgccc caggaaatcg
     1081 ccagcaacag cctgtcggcg caggttcgag tccttctcag gcatcgacat ccgggaccgt
     1141 gccatcgggc gatgaagcgg cgatccagaa cgcgcaggac cgcaagctcg ccttcacgaa
     1201 cgcgcccgtc gatcgtcgga cgacgagccc cgatcgcctg gcacggcccg catcgcccaa
     1261 tgtcttgcag gccggcaccg tcattcctgc ggccctcgtc accggcctca ggtccgatct
     1321 gcccggccag atcacggcgc aggtgacgga gaatgtctac gacacgccaa ctggtggatc
     1381 gctgcttatt cctcaaggag caagactgat cggtgtctac gacagtcagg tttcctttgg
     1441 gcagtcgcgt ctccttctgg tctggacgcg cctgattctt cccaatggct attcgatcgt
     1501 gctcgagcgc cagcctggtg ccgatgctgc cggccaagcg ggcctggagg atggggtcga
     1561 ccaccattgg ggatcgttgt tcaaggcggc gctgctctcg accattctcg ccgtcggcac
     1621 cgaacttggg tccgatcaga acgacagcga catcgtccgc gccttgcgcc gaggcaccgg
     1681 cgacaccctg aaccaggctg ggcagcaggt cgtgcggcgc aatctcaaca tccagccgac
     1741 gttgacgatc cgccccggct atccggtacg cgtgatcgtg actcgcgatc tcgtgcttcg
     1801 gccttatcag ggatagagag gcaacgatgt ccaagctgcg attgggcgcg atcctggacg
     1861 agaagcccgt gaagctgacc gttgacctgc cggccacggt ccatcgtgaa cttctggcct
     1921 atgccgagat actcgcccgc gagaccgggc aggcggtcga tccagcgaag ctcgtggcgc
     1981 ccatgctggg acgattcatg gcgacggatc gggcttttgc tagggagcgc cgcattgcac
     2041 gtcgcgtcga atc
//
LOCUS       DAOMEO010000596         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5726_length_2050_cov_1.972932, whole genome shotgun sequence.
ACCESSION   DAOMEO010000596 DAOMEO010000000
VERSION     DAOMEO010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5726_length_2050_cov_1.972932"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLH31_12760"
     CDS             <1..642
                     /locus_tag="PLH31_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQR90102.1"
                     /translation="TSEGLTSLQMDIKIAGITEEIMKQALAQAKGGREHILGEMNKAM
                     DAPREEVGDYAPKIETITIPTDKIREVIGTGGKVIREIVATTGAKVDINDEGTVKVSA
                     SDGAKIKAAIDWIKSITQEAEVGAIYDGKVVKVVDFGAFVNFFGAKDGLVHVSQISNE
                     RVAKPSDVLKEGQIVKVKLLGFDDRGKTKLSMKVVDQETGEDLSKKEAAAEEA"
     gene            complement(819..1430)
                     /locus_tag="PLH31_12765"
     CDS             complement(819..1430)
                     /locus_tag="PLH31_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90103.1"
                     /translation="MTMQFAEPQEQSGALLIAIVDETYGVSDDDDWTQAREVFRLNLE
                     KEFGLPFEEANIGPGADLPAFVTLLQTSQTSVLALLIALFFGGKPIKESLTAWRDMAR
                     KLLSFFPRRIFLNRQGAAVLAIDAVMEAMGGLPKSIRLLSYRNRHVHEDENLATIEAS
                     TEIAEPPATLYLGYVRHVFDIEADGVLFRVGVEGQSVAVSRLN"
     gene            complement(1460..>1640)
                     /locus_tag="PLH31_12770"
     CDS             complement(1460..>1640)
                     /locus_tag="PLH31_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90104.1"
                     /translation="RPVRLGTLQPPRDLRIAPTLEVGGRGTSGLDAFVLTSRKREEVD
                     NSSPRSIFFSDPSDF"
     assembly_gap    1641..1650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          439 a          600 c          620 g          381 t
ORIGIN      
        1 accagcgagg gtctcacctc gctgcagatg gacatcaaga tcgccggcat caccgaagag
       61 atcatgaagc aagcgctggc ccaggctaag ggcggtcgcg agcacatcct cggcgagatg
      121 aacaaggcga tggacgcgcc tcgtgaagaa gtcggcgact acgcgccgaa aatcgaaacc
      181 atcaccatcc cgaccgacaa gatccgggaa gtgatcggca ctggcggcaa ggtgatccgc
      241 gagatcgtcg ccaccaccgg cgccaaggtc gacatcaacg acgaaggcac ggtcaaggtt
      301 tcggcctcgg acggcgccaa gatcaaggcc gccatcgact ggatcaagtc gatcacgcaa
      361 gaagctgaag tcggcgcgat ctatgacggc aaggtcgtca aggtcgtcga tttcggcgcc
      421 ttcgtgaact tcttcggcgc caaggacggc ctcgtccacg tcagccagat cagcaacgaa
      481 cgcgtcgcca agccgtcgga cgtgctgaag gaaggccaga tcgtcaaagt gaagcttctc
      541 ggcttcgacg atcgcggcaa gaccaagctg tcgatgaagg tcgtcgacca ggaaacgggc
      601 gaagacctgt ccaagaagga agcggcggcc gaagaggcgt aagccttttc agcccggttt
      661 ttccggacgg aatgcagcgg gcggctccga aaggggccgc ccgttttgcg tttggggggt
      721 gtgtagaagc tgtcctgcgc gctcagtttg gtgacgacag aacaccataa ccgccatttg
      781 catggctcca aaaacggaca ctcgatgctc tgcgagctct aattcaggcg cgatacggcc
      841 acgctttgtc cttcaacacc cacgcgaaac agaactccat cggcctcaat gtcgaaaacg
      901 tgcctgacat agccaagata gagggttgcc ggtggctctg ctatttcggt cgatgcctct
      961 atagtggcga ggttttcgtc ctcgtggacg tgccggttcc gatagctgag caatctgata
     1021 gatttaggca atccgcccat ggcctccatc actgcgtcga tagcgagtac ggctgcgcct
     1081 tggcgattta gaaagatgcg acgtgggaaa aaggacagga gtttccgcgc catatcccgc
     1141 caagcggtga ggctctcttt aatcggcttg ccgccaaaga aaagggcgat aagcaacgcc
     1201 aacaccgagg tctgactggt ttgcagcaga gtgacgaaag ctggtaggtc agcgcctggg
     1261 cctatattgg cttcctcgaa cggtagacca aattcctttt ctagattcag acgaaaaact
     1321 tctcgggcct gggtccaatc atcatcgtcg ctgacaccat aggtttcatc gacaatggca
     1381 atgagcagcg ctccggattg ttcctgcggc tcggcaaatt gcatcgtcat ttggacacct
     1441 tggggcggct cgctaaatct caaaagtcag atggatcgct gaagaaaatg cttcttgggc
     1501 tgctgttgtc cacctcttca cgcttccgac tggtgagcac aaaggcatcg aggccagaag
     1561 tgccacgccc gccgacctcc agggtcggtg cgatccttaa atctcttgga ggctgcagcg
     1621 tgccgaggcg tacaggtctg nnnnnnnnnn aaggaccggt cttcgaccgg ccgctccgcg
     1681 gcggcccgaa gggccgtcct tgagagacct gtacgcctcg gcgatcgtct cgccgtgaga
     1741 tgtaaggatg gctgtgcccg cgagggctga cggttggcgc gcgcgttggc cggggtgggt
     1801 ttccccctcc tcgaccacgg ggatcgggat cgcgctgtcc ctcgatgggc gtggggatcg
     1861 agccccagat cctccccctg gcgggggagg tgtcggcgaa gccgacggtg gcggaagtcc
     1921 tgctgagtca atgcagtctt cccccaccgg cctccggccg cctcccccgt cagggggagg
     1981 acctacacgg gcgcccggga aatttatcag acgcgccgaa acctcccgca taatcatccc
     2041 acctcgtcgc
//
LOCUS       DAOMEO010000597         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5731_length_2049_cov_2.628385, whole genome shotgun sequence.
ACCESSION   DAOMEO010000597 DAOMEO010000000
VERSION     DAOMEO010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_2049_cov_2.628385"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>2049)
                     /locus_tag="PLH31_12775"
     CDS             complement(15..>2049)
                     /locus_tag="PLH31_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR90105.1"
                     /translation="DVRLWNGLLADLIRRGGIDRDYIDAHVNGFDAVEAALAEIDQSP
                     AAVAVDCGVALADLLTFFDLFTANPKTVSLFSMGANQSAQGVAKGLSILNAHLATGRI
                     GKPGACPFSITGQPNAMGGRETGGMANTLAGHMDFDPADRDRVARFWGAPNTTRKPGL
                     KAVDMFEAVHDGRIKAIWVMATNPAVSMPDASRVREALAKCPFVVVSDCIADTDTTAF
                     ADVKLPALAWGEKDGTVTNSERRISRQRALFAAPGQARPDWRIIADVAKAMGFDGFGW
                     GTQASVFREWARLTAYENEGRLLNLSGLTALGPDGYATLRPVQWPVAADGTGTARLFV
                     EGRFQTPDGRARMIPTRPRGPADAVDAVYPLSLNTGRIRDQWHTMTRTGLAADLCRHA
                     PEPFVEMHQVDAEAAGIREGALTRVVTRRGEAVALARITDRQRPGGVFMPMHWTDAFA
                     PSGRSNALIAPNVDPTSGQPEFKHTPARIRAYRETWKGFFLSRDILGAPTGQDLVWRR
                     IPQDACQQHEFAGRGDTDQRDAVRKVLSRGLIGEVVAYEDPGSGSRREAWVRDGRLVA
                     VLFMTTTGRLPPRDWLADLFSMPVLSRDARSALLFGRPPGAPVDKGPLVCVCLKVGAK
                     AVQAAIVAGALDPDAVGAVTGAGTNCGSCRPEIARMIATASLASPVKETVDVA"
BASE COUNT          311 a          685 c          753 g          300 t
ORIGIN      
        1 gaccgacgtt taggtcatgc gacatcgaca gtctccttga cgggcgaggc tagagaggcg
       61 gtggcgatca ttcgcgcgat ctccggccgg caggatccgc aattggtccc cgcccccgtg
      121 actgcgccga cggcgtcagg atcaagggcg ccagcgacga tggcggcctg cacggccttg
      181 gcgccgacct tcaggcacac gcagaccagc ggtcccttgt cgaccggcgc gcctgggggg
      241 cggccaaaca gcagggccga gcgggcgtcg cggctcagca ccggcataga gaacaggtca
      301 gccagccagt cgcgcggcgg caggcggccg gtggtggtca tgaacaggac ggcgaccaac
      361 cggccgtcgc ggacccaggc ctcgcggcgc gatcccgagc cggggtcctc gtaggcgacg
      421 acctcgccga tcagccccct ggacagaacc ttgcgcaccg cgtcgcgctg gtcggtgtcg
      481 ccccggccgg cgaactcgtg ctgctgacac gcgtcctgcg gaatgcggcg ccagaccagg
      541 tcctgcccgg ttggcgcacc aaggatatcc cgcgacagga agaagccctt ccaggtctcg
      601 cgataggccc ggatgcgggc gggcgtgtgt ttgaactctg gctggccgga ggtggggtca
      661 acattgggcg cgatcagcgc attggagcgg cccgacggcg cgaaggcgtc ggtccagtgc
      721 atgggcatga agacgccgcc aggacgctga cggtccgtga tcctggccag ggcaaccgct
      781 tcgccgcgcc gcgtgaccac gcgggtcagg gccccttccc ggatcccggc ggcttcggcg
      841 tcgacttggt gcatctccac gaagggctcg ggcgcatggc ggcacaggtc ggcggccagg
      901 cccgtgcggg tcatggtgtg ccactggtcg cggatgcggc cggtattgag cgacagcgga
      961 tagaccgcgt ccaccgcgtc ggcggggccg cgcggtcggg tcggaatcat gcgggcgcgc
     1021 ccgtcggggg tctggaagcg gccttcgaca aacaggcgcg ccgttcctgt tccgtcggcg
     1081 gcgaccggcc actgcaccgg ccgcagggtc gcgtatccgt ccggaccaag agcggtaagg
     1141 cccgacaggt tcagaagccg gccttcgttc tcataggccg tcaggcgcgc ccattcgcgg
     1201 aacacactgg cctgggttcc ccagccgaaa ccatcaaaac ccatcgcctt ggcgacgtcg
     1261 gcaatgatcc gccagtcggg gcgcgcctgc cccggcgccg cgaacagggc gcgctggcgc
     1321 gagatccggc gctcggaatt ggtgaccgtg ccgtccttct cgccccaggc cagggccggc
     1381 agcttcacgt cggcgaaggc ggtcgtgtcg gtgtcggcga tgcagtcgga gaccaccacg
     1441 aaggggcact tggccagagc ctcgcgcacc cggctggcgt cgggcattga gacggccggg
     1501 ttggtggcca tcacccagat cgccttgatg cgtccgtcat gcacggcctc gaacatgtcg
     1561 acggccttca ggcccggctt gcgcgtggtg ttgggcgcgc cccagaagcg ggcgacgcgg
     1621 tcgcgatcgg cggggtcgaa gtccatgtgg ccggccaggg tgttagccat gccgccggtc
     1681 tcgcgcccgc ccatggcgtt gggctggccg gtgatcgaaa acggacaggc gcccggcttg
     1741 ccgatgcggc cggtggcgag gtgggcgttg aggatcgaca gccccttggc cacgccctgg
     1801 gccgactggt tggcgcccat cgagaacagg ctgaccgtct tggggttggc cgtgaacagg
     1861 tcgaagaagg tcagcagatc tgctagcgcc acgccgcagt cgacggccac ggcggcgggc
     1921 gattggtcga tctcggccag ggcggcctcg acggcgtcga agccgttgac gtgagcatcg
     1981 atgtagtcgc gatcgatccc gccgcgccgg atcaggtcgg ccagtaggcc gttccacagc
     2041 cgaacgtcc
//
LOCUS       DAOMEO010000598         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5750_length_2045_cov_2.404020, whole genome shotgun sequence.
ACCESSION   DAOMEO010000598 DAOMEO010000000
VERSION     DAOMEO010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5750_length_2045_cov_2.404020"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLH31_12780"
     CDS             <1..823
                     /locus_tag="PLH31_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR90106.1"
                     /translation="GSARVIGGELTASPVVRKLTFTGSTEVGKTLMAQCAPTMKKISM
                     ELGGNAPFIVFADADLDAAVEGAIASKYRNNGQTCVCVNRILVHRDIAAAFEAKLAQA
                     VARLKVGPGSDAGVALGPLIDEAAVLKVEEHIADALGQGGRLVAGGRRHDLGHGFFEP
                     TVIGGCTPSMLIAHEETFGPVAALFEFETEAQALAMANDTEVGLAGYFYTRDIGRVWR
                     VAEALEVGMVGINTGLISTEVAPFGGIKESGTGREGSRHGLEDYSELKYLCMGGL"
     gene            complement(832..1575)
                     /locus_tag="PLH31_12785"
     CDS             complement(832..1575)
                     /locus_tag="PLH31_12785"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQR90107.1"
                     /translation="MTHTLTDVDLAAHLAETAGKILIQVRESGLFSDKALGKAGDQTA
                     NQFLVHALREQRPDDGLLSEEMKCDGSRLAQSRVWIIDPVDGTREYGEGRTDWAVHVA
                     LAIDGLATVGAVALPGLGLTLTTGKPQPLPPANNPPRMLVSRTRPAAEAVAVAEALGA
                     ELVPMGSAGAKAMAVVRGEADIYLHTGGQYEWDSCAPVAVAQAAGLHVSRVDGSPLRY
                     NREDPSLPDLLICRPELAERLLALVKAMP"
     gene            complement(1572..>2045)
                     /gene="cysC"
                     /locus_tag="PLH31_12790"
     CDS             complement(1572..>2045)
                     /gene="cysC"
                     /locus_tag="PLH31_12790"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HQR90108.1"
                     /translation="STIANEVEKRLALMNRHTFLLDGDNIRHGLNKDLGFTEADRIEN
                     IRRVGEVAKLMADAGLIVLTAFISPFRAERDMVRAMLPEGEFVEIFVDTPLEVAEARD
                     VKGLYKKARAGDLKNFTGIDSPYEPPLQPQIRVNTVEMTPAEAADFIIRQIMPLK"
BASE COUNT          327 a          654 c          708 g          356 t
ORIGIN      
        1 gggctcggcc cgggtgatcg gcggggaact caccgccagc ccggtggtgc gcaagctgac
       61 cttcacgggc tcgaccgagg tgggaaagac cctgatggcg cagtgcgcgc cgacgatgaa
      121 gaagatctcc atggaactgg ggggcaacgc cccgttcatc gtctttgccg acgcagacct
      181 ggacgccgcc gtcgagggcg cgatcgcctc gaagtatcgc aacaacggcc agacctgcgt
      241 ctgcgtgaac cgcatcctcg tccatcggga tattgctgca gccttcgagg ccaagctggc
      301 ccaggccgtg gccaggctga aggtcggccc cggcagtgac gcgggcgtgg ccctgggccc
      361 gctcatcgac gaggctgcag ttctcaaggt ggaggagcat atcgccgacg cgctgggcca
      421 aggcgggcgt cttgtcgctg gcggacgccg ccacgacctc ggccatggct tcttcgagcc
      481 cacggtgatc gggggctgca cgccatcgat gctgattgct cacgaagaga ccttcggtcc
      541 cgtcgccgcc ctgttcgagt tcgagaccga agcccaggcc ctggccatgg ccaatgacac
      601 cgaggtgggt ctggccggct atttctacac gcgcgacatc ggccgggtct ggcgggtggc
      661 cgaggcgctt gaggtcggca tggtcgggat caataccggc ctgatctcca ccgaggtcgc
      721 gccgttcggc gggatcaagg agtccggcac gggtcgcgag ggctcgcgcc atggtctgga
      781 ggactatagc gagctcaaat atctctgcat gggcggcctc tagactggcc gctacggcat
      841 cgccttgacc agagccagca agcgttcggc tagttccggc cggcagatca gcaggtcggg
      901 cagagagggg tcttcgcggt tgtagcgcag cggcgaacca tcaacgcgac tgacatgcag
      961 ccccgccgcc tgggccacgg cgaccggcgc gcagctgtcc cactcatact gaccgccggt
     1021 atggagatag atatcggcct cgccgcgcac gacggccatg gccttggcgc cggccgagcc
     1081 catggggacg agctcggcgc ctagcgcctc ggccacagcc acggcctcag cggcggggcg
     1141 ggtgcggctg accagcatgc gcggcgggtt gttcgccgga ggtagcggtt gcggcttgcc
     1201 cgtggtgagg gtcaggccaa ggccgggcag ggcgacggcc cccaccgtgg ccaacccgtc
     1261 aatggccagg gcgacgtgca ccgcccagtc ggttcgacct tcgccatact cgcgcgtgcc
     1321 gtcgacggga tcgatgatcc atacccggct ctgcgccagg cgggatccgt cgcacttcat
     1381 ctcttcggac agcaacccgt catcgggccg ttgctcgcgc agcgcatgga ccagaaactg
     1441 gttggcggtc tggtctccgg ccttgcccag ggccttgtct gagaacagcc cggactcgcg
     1501 aacctggatc aggatcttgc ctgcagtttc cgccaggtgg gcggcgaggt cgacatcggt
     1561 cagggtgtgg gtcatttcag cggcatgatc tggcggatga tgaagtctgc agcctcggcg
     1621 ggtgtcatct cgaccgtatt gacccgaatc tgtggctgca aaggcggctc gtaggggctg
     1681 tcgatgcctg tgaagttctt cagatcgccc gcgcgcgcct tcttgtagag gcccttgacg
     1741 tcccgcgcct cggcgacctc cagcggggta tcgacgaaga tctcgacgaa ctcgccctcg
     1801 ggcagcatgg cgcggaccat gtcgcgctcg gcccggaacg gcgagatgaa ggccgtaagc
     1861 acgatcaggc cggcgtcggc catcagcttg gcgacttcac caactcggcg gatgttttcg
     1921 atccggtcgg cctcggtgaa gcccaggtcc ttgttgagcc cgtggcggat attgtcgccg
     1981 tcgagcagga aggtgtggcg gttcatcaag gccagtcgct tttccacctc gttggcgatg
     2041 gttga
//
LOCUS       DAOMEO010000599         2043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5758_length_2043_cov_1.791247, whole genome shotgun sequence.
ACCESSION   DAOMEO010000599 DAOMEO010000000
VERSION     DAOMEO010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_2043_cov_1.791247"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..500)
                     /locus_tag="PLH31_12795"
     CDS             complement(144..500)
                     /locus_tag="PLH31_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HQR90109.1"
                     /translation="MMRKTLTVLAIGLSLAAAGAGAALAQTAAAKAAVDAGKAAGTVG
                     EQADGYLGIVSGGDAALRASVAEINTGRAAAYKDIAAKTGVTPEAAGQATAKQLYSRL
                     APGQYWKPLDGGWMKK"
     gene            complement(515..694)
                     /locus_tag="PLH31_12800"
     CDS             complement(515..694)
                     /locus_tag="PLH31_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HQR90110.1"
                     /translation="MRKRALLMGLIAMGALGACTPTIRVKVDPINIYAKLDADVRVRL
                     DKEVQTLIQQNPNLF"
     gene            complement(729..>2043)
                     /locus_tag="PLH31_12805"
     CDS             complement(729..>2043)
                     /locus_tag="PLH31_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HQR90111.1"
                     /translation="ALDEAQVAVEDGGGGLNASGGGGAMRADVKFDTGRLRDAAETRR
                     FNPINAAGRLSLSNGLWTGAIDATTPVGQPLGRIIIRHDVAQARGRAEIDASKLAFVK
                     DGLQPGDLSPMAAWATQASGPASFTGLFAWDADGMKSSGRLAANRIDFTSPIGFVATL
                     DGTIDFISLTPLVSAPDQTLKIVRIDSIIPLEGFAAAFSLGAEALNIPSAAFEAAKGR
                     ISIEPTEVSLDPDKPIRGVIVVEHLDLGELIAASSLVEKIQLDAVVDGRLPFELSARG
                     FRFLDGRIRAIQPGRLAISREALTGVQAGVQGQDSDSPGAPAPPPSPDAPVNAIQDFA
                     YQAMENLSFATLEAGVNSTDQGRLAILFHIKGEHDPKVAEKARIGLLDLIRGSAFNKR
                     IALPAKTPVELTLDTSLNFDELLQAWRRAYKGEDPEASRSAPVQP"
BASE COUNT          281 a          679 c          732 g          351 t
ORIGIN      
        1 gggttccagt cgcgagatcc tcccctcttg ggggaggtgg cccaaagggc cggggggggg
       61 gctagcgcgg ctttcgccca gatggccccc tccgtcgcct ccggcgacac ctcccccaga
      121 gggggaggag ctttcttgat cgcctacttc ttcatccagc cgccatccag cggcttccag
      181 tactggccgg gcgccagacg gctgtaaagc tgcttagcgg tggcctggcc ggcggcttca
      241 ggggtcacgc cggtcttggc ggcgatgtcc ttgtaggcgg cggcgcggcc ggtgttgatc
      301 tcggcgaccg aggcccgcag ggcggcgtcg cccccggaca cgatccccag atagccgtcc
      361 gcctgctcgc ccaccgttcc ggcggccttg ccagcgtcga cggcggcctt ggcggcggcc
      421 gtctgggcca gggccgcgcc cgcgcctgcg gcggcgagtg acaggccgat ggccagaacc
      481 gtcagtgtct tgcgcatcat gtcggttcct ctgtctagaa caggttgggg ttttgctgga
      541 tcagcgtctg gacttccttg tccagccgca cccggacgtc ggcgtccagc ttggcgtaga
      601 tattgatcgg gtcgaccttc acgcggatcg tcggggtgca ggctcccagc gctcccatcg
      661 cgatcaggcc catcagcagg gcgcgctttc tcatgcgggt gtctccagtt cggttggaat
      721 gtgatcggtc acggctgaac cggggctgaa cgcgaagctt cagggtcctc gcccttatag
      781 gcgcgtcgcc aagcttgcag aagctcgtcg aagttcagcg aggtgtcgag ggtcagttcg
      841 accggggtct tggccggcag ggcgatgcgc ttgttgaacg ccgagccgcg gatcaggtcc
      901 agcaggccga tgcgggcctt ttcggcgacc ttgggatcgt gctcgccctt gatgtggaac
      961 aggatcgcca gccggccctg atcggtgctg ttgacgcccg cctccagggt ggcgaacgac
     1021 aggttctcca tggcctggta ggcgaaatcc tggatcgcgt tgaccggggc gtccggagac
     1081 ggcggcggcg caggcgcgcc ggggctgtcg ctgtcctggc cctgaacacc ggcctgaacg
     1141 ccggtcaggg cctcgcgcga gatcgccagc cggccgggct ggatggccct gatcctgccg
     1201 tccaggaacc ggaagcccct ggcgctgagt tcgaacggca ggcggccatc aaccacggcg
     1261 tccagctgga tcttctcgac cagggacgag gcggcgatca gttcgccgag gtccaggtgc
     1321 tcgacgacga tcacgccgcg gatcggcttg tccggatcca gcgacacctc ggtgggctcg
     1381 atcgaaatcc ggcccttggc ggcttcgaag gcggccgagg ggatgttcag cgcctccgcg
     1441 cccaggctga aggcggcggc gaagccttcc agcgggatga tactatcgat gcggacgatc
     1501 ttcagggtct ggtcgggcgc gctgaccagc ggggtcaggc tgatgaagtc gatggtcccg
     1561 tccagggtag cgacaaagcc gatcgggctg gtgaagtcga tcctgttggc cgccagccgg
     1621 ccgctgctct tcatgccgtc ggcgtcccag gcgaacaggc ccgtgaagct ggcgggtccc
     1681 gaggcctggg tggcccaggc cgccatcggc gacagatcgc ccggctgcag gccgtccttc
     1741 acgaaggcca gtttactggc gtcgatctcg gcccgtccgc gcgcctgggc gacatcgtgg
     1801 cggatgatga tgcggcccag cggctggccg accggcgtgg tcgcatcgat ggccccggtc
     1861 cacaggccat tcgacagaga caggcggccc gcggcgttga tcgggttgaa gcggcgggtc
     1921 tcggccgcgt cgcgcagccg gcccgtgtcg aacttcacgt ccgcgcgcat cgcgccgccg
     1981 ccgcccgagg cgttcaggcc gccgccgccg tcctcgacag cgacctgcgc ctcgtcgagc
     2041 gcg
//
LOCUS       DAOMEO010000600         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5762_length_2042_cov_2.593357, whole genome shotgun sequence.
ACCESSION   DAOMEO010000600 DAOMEO010000000
VERSION     DAOMEO010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5762_length_2042_cov_2.593357"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>751
                     /locus_tag="PLH31_12810"
     CDS             <1..>751
                     /locus_tag="PLH31_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90112.1"
                     /translation="GWPYTQKTKGYAVFGQADYRFTDKLTGTVGLRWSADDKHMNYQS
                     QIEDGLIVLLTSDQSKTFSALSGRLGLRYEVTPDASVYATYNRGYKSGGFFGGLATTP
                     EEVEPYDNETLDAFELGAKTEFFDRRLRLNVSGFYYDYKNQQVFAQALRNGLSVLVLD
                     NAANSEVYGGEAELTARPIQPLTLTAGVSLLHAKYGEYFSEGQDFSGNWLPQSPKVTF
                     NAAATYVAALPSGGAVVANVDANYASRVYFDN"
     assembly_gap    752..761
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <762..963
                     /locus_tag="PLH31_12815"
     CDS             <762..963
                     /locus_tag="PLH31_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90113.1"
                     /translation="SQDAVWLAGAQLSWRSPDQAVEAGVFARNLFDKSYVVSISNIDS
                     LGEDLLSMNRPRSLGAFLRYHY"
     gene            1060..1746
                     /locus_tag="PLH31_12820"
     CDS             1060..1746
                     /locus_tag="PLH31_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90114.1"
                     /translation="MARRSAANSPAPSLDTKSRPSRQRGIDTFEVILTTAGELLAEVG
                     FERLTTNLVCQRAGLTPPALYRYFPNKYALLKELAHRLMDAQDQVVLAWLANGGTRFT
                     SLEDSVAKNRDLQKQVNAVTRAFPGGDWILRALRALPVLQEVRTTSRDTIALALFDLL
                     RQANPGVDEDRLHTATRLTTQMMYAATEMVLEEPDQDEDRINEEICWMVSLYYQHLTR
                     PASAPATRAG"
     gene            complement(1685..>2042)
                     /locus_tag="PLH31_12825"
     CDS             complement(1685..>2042)
                     /locus_tag="PLH31_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90115.1"
                     /translation="GVGLINAVILAVLWSGPGRGLFVACVAAFGFLWLFALPFQTLLL
                     IRLDPTRRAAMQLGAAQLLGSSAGPLAASLVVGDGEVGRALALSAVCLTAALALISLL
                     ASRARTPVASGADSTG"
BASE COUNT          367 a          732 c          630 g          303 t
ORIGIN      
        1 cggctggccc tacacccaga agaccaaggg ctacgctgtc ttcggtcagg ccgactaccg
       61 gttcaccgac aagctgaccg gcacggtggg cctgcgctgg tcggctgacg acaagcacat
      121 gaactatcag agccagatcg aggacggtct gatcgtcctg ctgacctccg accagagcaa
      181 gaccttctcg gcgctctccg gacgactggg cctgcgctac gaggtgacgc ccgacgccag
      241 cgtctacgcc acctataatc gcggctacaa gagcgggggc ttcttcggcg gtctggccac
      301 cacgcccgag gaggtcgagc cctacgacaa cgaaaccctc gacgccttcg agcttggcgc
      361 gaagacggag ttctttgacc gtcgcctgcg cctgaacgtg tcgggcttct attacgacta
      421 caagaaccag caggtcttcg cccaggcgct gcgcaacggc ttgtcggtgc tggtgctcga
      481 caacgccgcc aactccgagg tgtatggcgg cgaggccgag ctgaccgcgc ggccgatcca
      541 gcccctgacc ctgacggccg gcgtctcgct gctgcacgcc aagtacggcg aatatttcag
      601 cgagggccag gacttcagcg gcaactggct gccgcagtcg cccaaggtga cgttcaacgc
      661 cgccgccacc tatgtcgcgg ccctgccgtc gggcggcgcg gtcgtggcca atgtcgacgc
      721 caactatgcg tccagggtct atttcgacaa cnnnnnnnnn ngtcacagga cgcggtgtgg
      781 ctggccggcg cccagctcag ctggcgttcg cccgaccagg cggtcgaggc cggcgtgttc
      841 gcccgcaacc tgttcgacaa gtcgtatgtg gtctcgatct ccaacatcga cagcctgggc
      901 gaggacctgc tgagcatgaa ccgtccgcgc agcctcggcg ccttcctgcg ctaccactac
      961 taggctcgcg tccccgcgac gcggcctttg cgcctcgtcg cgggaagggg ttcttgctat
     1021 ctgtcggcga tcctaagatc cgggccgttc aggaacccca tggcaagacg ctccgccgcc
     1081 aacagtccgg cgccctctct ggacaccaag tcgcgcccgt ccaggcagcg cggcatcgac
     1141 acgttcgagg tcatcctgac gacggcgggc gagctgctgg ccgaggtggg tttcgagcgc
     1201 ctgaccacca accttgtgtg ccagcgcgcg ggcctgactc cgccggccct ctatcgctac
     1261 ttccccaaca agtacgccct gctaaaggag ctggcccatc gcctgatgga cgcccaggat
     1321 caggtggtgc tggcctggct ggcgaacggc ggcacgcgct tcacgtcgct ggaagactcg
     1381 gtcgccaaga accgcgacct gcaaaagcag gtcaatgctg tgacccgcgc cttccccggc
     1441 ggcgactgga tcctgcgagc gctacgcgcg ctgccggtgc tgcaggaggt gcgcaccacc
     1501 tcacgcgaca ccatcgccct ggccctgttc gacctgctgc gccaggccaa tcccggggtc
     1561 gacgaggacc gccttcacac ggccacccga ctgaccaccc agatgatgta cgccgccacc
     1621 gaaatggtgc tggaagagcc cgaccaggac gaggaccgga tcaacgaaga gatctgctgg
     1681 atggtcagcc tgtactatca gcacctgacg cgaccggcgt ccgcgcccgc gacgcgagca
     1741 ggctgatcag cgccagggcc gccgtcaggc agacggccga cagggccaac gcccgcccca
     1801 cctcgccatc ccccactacc agcgacgccg ccagcgggcc cgcgctgctc cccagcagct
     1861 gggccgcgcc caactgcatc gcggcgcgtc gcgtcgggtc cagacggatc aacagcaggg
     1921 tctggaacgg caaagcaaat aaccaaagga agccgaacgc cgccacgcag gccacgaaca
     1981 ggcctcgccc ggggccgctc cacagcaccg ccaggatgac ggcgttgatc aggcccacgc
     2041 ca
//
LOCUS       DAOMEO010000601         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5780_length_2035_cov_2.197980, whole genome shotgun sequence.
ACCESSION   DAOMEO010000601 DAOMEO010000000
VERSION     DAOMEO010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_2035_cov_2.197980"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PLH31_12830"
     CDS             complement(<1..179)
                     /locus_tag="PLH31_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQR90116.1"
                     /translation="MKVLVPVKRVIDYNVKVRVKADQTGVDLANVKMSMNPFCEIAVE
                     EAVRLKEKGVATEIV"
     gene            412..690
                     /locus_tag="PLH31_12835"
     CDS             412..690
                     /locus_tag="PLH31_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90117.1"
                     /translation="MNRTINRLKIIFIGIFLLSSAGVFGYHYLWVWPKERCEARGGAW
                     AGKWLKCGTIYSIETLTRRPLNVPPINGEAATPAAPATPAPASPPEKK"
     gene            complement(692..>1248)
                     /locus_tag="PLH31_12840"
     CDS             complement(692..>1248)
                     /locus_tag="PLH31_12840"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQR90118.1"
                     /translation="TRTGDGGSTRLATGEPVSKASLRVDAYGGVDETNAFLGVARQHN
                     AHDPELDAMLERIQNDLFDLGADLATPEQHEKPAWEPLRVVESQVERLEQEIDRMNSE
                     LSSLTSFILPAGSPASAALHVARTVCRRAERVVVLLSETEGEVVGKPALKYLNRLSDL
                     LFVAARWANDKGASDVLWKPGATR"
     assembly_gap    1249..1258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1315..1512)
                     /locus_tag="PLH31_12845"
     CDS             complement(1315..1512)
                     /locus_tag="PLH31_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HQR90119.1"
                     /translation="MDRVFDFLIPAALLAVLLALSGGLYALFRGGDFGRSNSNKLMRL
                     RVVLQFVAIIVLVAAYWWRTR"
     gene            1825..>2035
                     /locus_tag="PLH31_12850"
     CDS             1825..>2035
                     /locus_tag="PLH31_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90120.1"
                     /translation="MAKAALAAAAFSASEREDAMAEVRQLITDLRDAAQDGRQLARRY
                     ADVLQNVVDEYCTEFEKRAEAALARL"
BASE COUNT          369 a          662 c          613 g          381 t
ORIGIN      
        1 atgacgatct cggtcgccac gcccttttcc ttgagacgca cggcttcttc gaccgcgatc
       61 tcgcagaacg ggttcatgga catcttgacg ttcgccagat cgacgcccgt ctggtccgct
      121 ttgacgcgaa ccttcacgtt gtaatcgata acccgtttaa ccgggactag gaccttcatc
      181 gggtttcacg cctccaggct gcgtttttat cctgcgtggg caggggaatt accctttttg
      241 caccgcaacg caagtttccc ttggcgtaag cgtaaagctg catcacgcgt cgggttcaaa
      301 caggtgtacg acacttcgtc ggcgccgtcc gccacggtca acgcgccgca gggcaaaggg
      361 ccgcgcgaaa ggactggtca ggctttcgct cgccccctaa acagccgggc catgaaccgc
      421 acgatcaatc gcctcaagat catctttatc ggcatcttcc tgctcagcag cgccggggtg
      481 tttggctatc actacctctg ggtctggccc aaggagcgct gcgaggcgcg tggcggagcc
      541 tgggccggca agtggctcaa gtgcggcacc atctattcga tcgagaccct cacgcgccgg
      601 cccttgaacg tgccgccgat caacggcgag gctgccacgc cggctgcgcc cgcgacgccc
      661 gcgcccgcgt ctccgcccga gaagaaatag tctagcgcgt cgcgccgggc ttccagagca
      721 cgtccgacgc gcccttgtcg ttggcccagc gggcggccac gaacaacagg tccgacaggc
      781 ggttgagata tttcagcgcc ggcttgccca cgacctcgcc ctcggtttcc gacagcagca
      841 ccacgacgcg ttcggcgcgc cgacagaccg tccgggccac atgcagggcg gccgaggcgg
      901 gtgatccggc cggcaggatg aacgaggtca gggaggacag ctcgctattc atgcggtcga
      961 tctcctgctc caggcgttcg acctggctct cgacgacgcg cagcggctcc caggccggct
     1021 tctcatgctg ctccggcgtg gccagatccg cgcccaggtc gaaaaggtcg ttctggatgc
     1081 gctccagcat ggcgtcgagt tctggatcat gtgcgttgtg ttgtcgcgcc acgcccagga
     1141 aggcgttggt ctcatcgaca ccgccatagg cgtccactct cagcgacgcc ttgctgacag
     1201 gctctcccgt cgccagccgc gtcgagccgc catcacccgt gcgggtgtnn nnnnnnnngc
     1261 gggtgtagat tctgttgagc gtgaccatgg gtcgcggacc gccgttatcg cattctagcg
     1321 cgtgcgccac caatatgcgg ccaccagcac gatgatcgcc acgaactgca gcaccacgcg
     1381 gagccgcatc agcttgttgg aattggaacg gccaaagtcg ccgccccgga agagggcgta
     1441 gagaccgccg ctgagcgcga ggagcaccgc caggagcgcg gccggtatga gaaagtcgaa
     1501 tactctgtcc atgggcagaa gataggcgtt cgagcggaac tttgccacac cgctgcgcat
     1561 tttgcggaac gctgtccaat gagcgaattc gccgaacagg ttcgctgagc gcttttgtcg
     1621 cactgcacaa tcccagtgcc cgcatatata tcaaaaccag ccgatgttcg gtgattttag
     1681 ttctgtctag cggttgcaat ggcgccagta cggcgatatg gcccccgctc cgattttcgt
     1741 agtatgcgtt cgttgaacat gatcgccacc tcaccgcagg tcgttcaacg ctttttcccc
     1801 agcggggagg caggagaaag aattatggca aaggccgctt tagccgccgc cgcgttctcc
     1861 gcgtccgaac gcgaagacgc catggcggaa gtccgccagc tgatcacaga cctgcgcgac
     1921 gctgcgcagg atggccgcca gctggcgcgc cgctacgccg acgtcctgca aaacgtcgtc
     1981 gatgagtatt gcaccgagtt tgagaagcgc gccgaagccg ccctcgcccg cctgg
//
LOCUS       DAOMEO010000602         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5792_length_2032_cov_2.743045, whole genome shotgun sequence.
ACCESSION   DAOMEO010000602 DAOMEO010000000
VERSION     DAOMEO010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5792_length_2032_cov_2.743045"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLH31_12855"
     CDS             complement(<1..337)
                     /locus_tag="PLH31_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR90121.1"
                     /translation="MIRLDNISKQNGHQILFIEASMGVQKGEKVGLVGPNGAGKTTLF
                     RMITGQEQPDDGLVVIDPGMTIGYFSQDVGEMAGQSAVAAVMDGVGPVSALAAEMARL
                     EAAMVDPDQA"
     gene            606..932
                     /locus_tag="PLH31_12860"
     CDS             606..932
                     /locus_tag="PLH31_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQR90122.1"
                     /translation="MRETMLNAFPQGAVHEAGEDFQASVRSDPRVFALWEGLTPLGRN
                     EFICWVDDAKQPATRQRRIERTRAALMEGKKRPCCWSGCIHRMDKAPSAWQQAVLIDR
                     KLKNER"
     gene            complement(1014..1730)
                     /locus_tag="PLH31_12865"
     CDS             complement(1014..1730)
                     /locus_tag="PLH31_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90123.1"
                     /translation="MRRYAGAATQGAAVGPNDVYSVSNFRLIRFDKKTGEKRSEWSGD
                     KARFPHINSCAVINEELVCASSNFPATPQVSTVEVFDSMSLAHKRSIALGLGTGSVTW
                     IDRHGGFWWAMFANYDGRGGEAPRDHRHTTLVKFDDQWRRLEAWGLPSTVLGRIAPMS
                     ISGGGWGADGKLYLSGHDLPELYVMQLPKGGGVLDHLDTVGMEAEGQAIDWDERQPGM
                     LYGITRRTREILAMRLPLTR"
     gene            complement(1678..>2032)
                     /locus_tag="PLH31_12870"
     CDS             complement(1678..>2032)
                     /locus_tag="PLH31_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90124.1"
                     /translation="RLSISLMASTCLLAPAFAQDVDATSVEAIIVTGKREAQRAAIAV
                     KRETFSVSDVVSADEIGKLPDHNTAAALPLFAGSRGGPGAGGPGARRGRADPKSPDCA
                     SLCRRRHSRRRGRAK"
BASE COUNT          383 a          639 c          643 g          367 t
ORIGIN      
        1 cggcctggtc cggatcgacc atggccgcct ccagcctggc catttcggcg gccagcgcgc
       61 tgacgggacc gacgccgtcc ataaccgcgg cgaccgcgct ctggcccgcc atttcgccga
      121 catcctggct gaaatagccg atcgtcatgc cgggatcgat cacgaccagg ccgtcgtcgg
      181 gctgctcctg gccggtgatc atgcggaaca gcgtcgtctt gcccgcgccg ttggggccga
      241 caagcccgac cttttcaccc ttctggacgc ccatcgaggc ttcgatgaac aggatctggt
      301 ggccgttctg cttggagata ttgtcaaggc ggatcatggg cggtctgcta acgcgggcgg
      361 aagcagaacg ccagccttag agctccagtc cacccacaaa gccggacttg gatctggtca
      421 agcagggcgc tgggctgagt gaggccgttg ggtcgctccg atgcttgacc cttctcaccc
      481 gaccccggaa agtggtgcgc cctggaccgt gggaaatggc cggcagacac cgcgctccag
      541 catattccac gcttgccggg taggcgctga tacgcgatgt cgggagtaga gcgaggggca
      601 aagccatgcg tgagacgatg ctgaacgcgt ttccccaagg cgccgttcat gaggctggcg
      661 aggatttcca ggcatcggtg cgatcggatc cgagggtctt tgcgctctgg gagggcctga
      721 cgccgcttgg tcgaaacgag tttatttgct gggtcgatga cgccaaacag ccagcaaccc
      781 gccagcgacg gattgagcgt acccgcgccg cactgatgga gggtaagaag cgaccatgct
      841 gctggtcagg ctgtattcac cgaatggata aagcgccaag cgcttggcaa caggcggtac
      901 tgatcgatcg gaaattgaaa aacgaacgat aggccggccg ataaccgaga cctctatttt
      961 ccattccctg taaactgctg cgccagcaac tgccccggaa cgactgcaaa gtgctatcgc
     1021 gttagcggga gacgcatggc gaggatctct cgggttcgcc tcgtaatccc gtagagcatt
     1081 cccggctggc gctcgtccca atcgatggcc tgcccttcag cctccatgcc cacggtgtcg
     1141 aggtgatcaa gcacgccacc gcctttcggc agttgcatga catagagttc gggcaggtcg
     1201 tgaccgctca gataaagttt gccgtccgcc ccccagccac cgcctgagat actcatcggc
     1261 gcgatgcgcc ccagcaccgt ggacggcaga ccccaggcct caagccgccg ccactggtcg
     1321 tcgaatttca ccagcgtggt gtgcctatga tcacgcgggg cctccccgcc cctgccgtca
     1381 tagttggcga acatggccca ccagaaaccg ccgtggcgat cgatccaggt gaccgagccc
     1441 gtgccgaggc caagcgcgat actgcgcttg tgggccaggc tcatggagtc gaacacctca
     1501 accgtactga cctgaggcgt ggcggggaaa ttggagctgg cgcaaacaag ctcctcgttg
     1561 atcacggcgc aggagttgat gtgcggaaag cgcgccttat cgccactcca ctcagaccgc
     1621 ttctcgccgg tcttcttgtc aaagcggatc agcctgaaat tgcttacaga gtagacgtca
     1681 tttggcccga ccgcggcgcc ttgagtggcg gcgccggcat aacgacgcac agtctgggct
     1741 cttcggatcg gcgcggccgc ggcgggcgcc gggacctccg gcgcctggac cgccacggct
     1801 gccagcaaat agaggcaggg cggcggcggt gttgtgatca ggcagtttgc cgatctcgtc
     1861 cgccgagacg acatccgaca cggaaaaggt ctcgcgttta acggcgatgg cggcgcgctg
     1921 cgcttctcgc tttccggtca cgataatcgc ctcgaccgac gtggcgtcaa cgtcctgggc
     1981 gaaggcgggc gccaagaggc aggtgctagc catcaggctg atggaaagtc tg
//
LOCUS       DAOMEO010000603         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5797_length_2031_cov_2.443320, whole genome shotgun sequence.
ACCESSION   DAOMEO010000603 DAOMEO010000000
VERSION     DAOMEO010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5797_length_2031_cov_2.443320"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLH31_12875"
     CDS             <1..510
                     /locus_tag="PLH31_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR90125.1"
                     /translation="RIDIVYNNASAARFGSIADLSIEDWRFTIRNELDLVFLTTKFAW
                     PHLAKQGGAIINVASTAGWQGSRGNGTIAHNATKGGVIAMTRQMALEGAPLGIRANSI
                     SPGFIITPGTRAFVENPTVRAQLTASIPLGRPGEPEDVVGMAAFLASDEAAFITGADI
                     IIDGGTTAC"
     gene            538..1452
                     /locus_tag="PLH31_12880"
     CDS             538..1452
                     /locus_tag="PLH31_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR90126.1"
                     /translation="MDIRGIGYLGFESPNIDAWRSYGPEVLGLAIAPSPEDEPEALYL
                     KMDDRRYRFAFQPGPIDKLAYIGWECVNRIAFEKAVKKLQDAGIAVEQGSDDLKARRA
                     VRDVVRFKDPVGYQYELFYGQKGEPDSFVPGRRHGGFNTYGRGFGHVVVMTPEYGPEL
                     DDFLINIMGFQWYGSGAGKGRTGFYRAGLNNLTSHDIGYGHAPGRMGIQHIGLLTGDL
                     RDVGETWDIVNKRQIQVQMTLGQHTQDPHFSFYHFSPSGFAVEVIAETHPWPGDPFEL
                     NAERLSYWGHQLVGPILGTTIRTPEELR"
     gene            1452..>2031
                     /locus_tag="PLH31_12885"
     CDS             1452..>2031
                     /locus_tag="PLH31_12885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR90127.1"
                     /translation="MGLLDGKVALVTGGGGGIGRGIARKFIREGATVLVAEFNDEYCA
                     SIKKELTEELGGRAEVIKADVRVKEQIQGAVQAAIDLFGGIDILVNNAFTLSPKVLLE
                     QKTDEMLDSTLHSGLWAGWWAMQAARPHMAARGGGSIINFYSIDVETAAWLNADYNIT
                     KSALRGLTRSAAHEWGRFNIRVNLLAPAAMGTV"
BASE COUNT          383 a          662 c          650 g          336 t
ORIGIN      
        1 cggatcgaca tcgtctacaa caacgcctcg gcggcgcgct tcggatcgat cgccgacctg
       61 tcgatcgagg actggcgctt cacgatccgc aacgaactgg acctcgtgtt cctgacgacg
      121 aagttcgcct ggccgcatct ggcgaaacag ggcggggcga tcatcaacgt cgcctcgacc
      181 gccggctggc aagggtctcg cggcaacggc acgattgccc acaacgccac caagggcggc
      241 gtcatcgcca tgacgcggca gatggcgctg gagggcgcgc ccttgggcat acgcgccaac
      301 agcattagcc ccggcttcat catcacgccc ggaacgcgcg ccttcgtcga gaacccgacc
      361 gtgcgcgccc agctgacggc cagcattccc cttggtcgtc ctggcgagcc agaggacgtc
      421 gtcggcatgg ccgccttcct ggccagcgac gaggccgcct tcatcaccgg cgccgacatc
      481 atcatcgatg gcggcacgac cgcctgctag ccaaccgaca agacgaggat ctggaccatg
      541 gatatccggg gtatcggcta tctgggcttc gaaagcccca acatcgacgc ctggcgcagc
      601 tatggccccg aggttctggg cctggccata gcgccatcgc ccgaggacga gcccgaggca
      661 ctctatctga agatggacga ccggcgctat cgcttcgcct tccaacccgg tccgatcgac
      721 aagctggcct atatcggctg ggaatgcgtc aaccggatcg cgttcgagaa ggccgtcaag
      781 aagctgcagg acgcgggaat cgcggtggag cagggcagcg acgacctcaa ggcccgacga
      841 gccgtgcgcg acgtggtccg gttcaaggat cccgtgggtt atcagtacga acttttctac
      901 ggccagaagg gcgagcccga ctcgttcgtg ccggggcgtc gccatggcgg attcaacacc
      961 tatggccggg gctttggcca cgttgtggtg atgacgccgg aatatggtcc ggaactggac
     1021 gacttcctga tcaatatcat gggtttccag tggtatgggt cgggcgccgg caagggcagg
     1081 accggcttct atcgcgccgg gctcaacaac ctgaccagcc acgacatcgg ttatggtcac
     1141 gcgcctggtc ggatgggcat ccagcacatt ggcctgttga ccggcgatct ccgcgacgtc
     1201 ggcgaaacct gggatatcgt caacaagcgc cagatccagg tacagatgac cttgggccag
     1261 catacccagg atccgcattt ctccttctat cacttcagcc cgtcgggctt cgccgtcgag
     1321 gtcatcgccg agacccaccc ctggcctggc gacccgttcg agctgaacgc cgagcgcctc
     1381 agctactggg gtcaccaact ggtcggaccc attctcggca cgacgattcg cacgcccgag
     1441 gaactgcgct gatgggactc ctcgacggca aggtcgccct ggtcacgggc ggtggcggcg
     1501 gcatcggccg gggcatcgcc cgaaaattca tccgcgaggg ggcgaccgtt ctggtcgccg
     1561 aattcaacga tgaatactgc gcctccatca agaaggagct gaccgaggaa ctcggcggac
     1621 gcgccgaggt catcaaggcc gatgtgcgcg tcaaggaaca gatccagggc gcggttcagg
     1681 cggcgatcga cctgttcggc ggcatcgaca tcctggtcaa taacgccttc accctcagcc
     1741 ccaaggtcct gctggagcag aagaccgacg agatgctgga cagcacgctc cactcggggc
     1801 tctgggcagg ctggtgggcg atgcaggcgg cgcgacctca catggcggcg cgcggcggcg
     1861 gctcgatcat caacttctac tcgatcgacg tggagacggc cgcctggctg aatgccgact
     1921 acaacatcac caagtcggcg cttcggggcc tgacgcgcag cgcggcgcac gagtggggtc
     1981 ggttcaacat ccgggtcaac ctattggcgc cggcggcgat gggcacggtc t
//
LOCUS       DAOMEO010000604         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5808_length_2028_cov_2.693360, whole genome shotgun sequence.
ACCESSION   DAOMEO010000604 DAOMEO010000000
VERSION     DAOMEO010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_2028_cov_2.693360"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLH31_12890"
     CDS             <1..542
                     /locus_tag="PLH31_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR90128.1"
                     /translation="SMLMERGQVISPICATPDMLDAEDAGGGAIAMRLGVKPPLSWPP
                     EHNDTATRAWMRRLLTETPDEPGYVCWYMIAQNRLVGICGFKGPPTDKGEVEIGYSVV
                     EAEHRKGYGVEAAGLLVARAFRDPRVDVVTAETLPRLIASQKVLARCGFTHVGGYIDA
                     EDGQIMRFEMRRPSERPGA"
     gene            complement(496..>2028)
                     /locus_tag="PLH31_12895"
     CDS             complement(496..>2028)
                     /locus_tag="PLH31_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90129.1"
                     /translation="ELLVEAYIDQAEGRGASAFQKLDRWRRVDRAENDRAHSSSVAEM
                     SAALESELSAKRDESRRLAAEVELNRRLAAASGVIAVLLALLVLGGVSWAFNQRRISR
                     SLRDARAKAEAANQAKSAFLAVMSHELRTPLNGMLGLAQSLRSEALGDHQREQVDLIL
                     DSGDTLLVLLNDILDLSKIEAGKLEIAPTAGDLVQVCARLIGGYQTTARDKGIELIFR
                     VEGVAPGPLMFDAVRLRQCLTNLVSNALKFTNAGRVEVILAAGPDLTTGRQRIRLRVT
                     DTGIGMSEVTLAKLFRAFTQADASTTRTFGGTGLGLNITRRLVEMMGGEIRVESVEGV
                     GSTFIVEMLADACGADSEAPVDAEAPEETSEPSFAALHGRRVLVVDDHPVNRRVIRLF
                     LTPFECDLVEAENGREALDALEAGPIDIVLMDVNMPVMDGLEATRRLRAEPRFAKLPV
                     IALTADVMSAQIKTCLAAGADAHVAKPIDLRALLSAMDQALSPRLPTPRGAQTGAASQ
                     SA"
BASE COUNT          321 a          695 c          685 g          327 t
ORIGIN      
        1 gatcgatgct catggaacgc ggccaggtca tttcaccgat ctgcgcgaca cccgacatgc
       61 tcgacgcgga agacgccggc ggcggcgcca tcgccatgcg gctgggcgtc aagcctccgc
      121 tttcctggcc gccggagcat aacgacaccg ccactcgcgc ctggatgcgc cgcctgctca
      181 ccgagacgcc ggacgagccc ggctatgtct gctggtacat gatcgcccag aatcgcctgg
      241 tcggcatctg cggtttcaag ggacctccga ccgacaaggg cgaggtcgag atcggctact
      301 ccgtggtcga ggccgagcat cgcaagggct atggcgtcga ggccgctggc ctgctggtgg
      361 cgcgggcctt ccgggatccc cgggtagacg ttgtcacggc cgagaccctg cccaggctga
      421 tcgcctcgca gaaggtgctg gcccgctgcg gctttactca tgtcggcggc tatattgacg
      481 ccgaggacgg ccagatcatg cgctttgaga tgcggcgccc gtctgagcgc cccggggcgt
      541 aggcaggcgc ggcgacaagg cctggtccat ggccgacagc agggcgcgca ggtcgatcgg
      601 cttggcgaca tgggcgtcgg cccccgcggc caggcaggtc ttgatctgag ccgacataac
      661 gtcggcggtc agggcgatga ccggcagctt ggcgaaacgc ggctcggcgc gcagacgccg
      721 cgtggcttcc agtccgtcca tcacgggcat gttgacgtcc atcagcacga tgtcgatggg
      781 gccggcttcc agggcgtcca acgcttcgcg tccgttttcc gcctcgacca ggtcgcactc
      841 gaacggcgtc aggaacaggc ggatcacgcg gcggttcacg ggatgatcgt ccaccaccag
      901 cacgcggcgg ccatgcaggg cggcgaagga cggctcgctg gtctcctcag gcgcttcggc
      961 gtcgaccggc gcctcgctgt cagccccgca ggcgtcggcc agcatctcga cgatgaaggt
     1021 cgagcccacg ccctccacgc tctcgacccg gatctcgccg cccatcatct cgaccagccg
     1081 gcgggtgatg ttcaggccca gccctgtgcc gccgaaggtg cgggtggtcg aggcgtcggc
     1141 ctgggtgaag gcccgaaaca gcttggccag agtcacttcg ctcatgccga tgccggtgtc
     1201 tgtcacccgc aggcgaatcc gctggcgacc agtcgtcagg tccggcccgg cggccagaat
     1261 cacctcgacc cgcccggcat tggtgaactt cagggcgtta gacacgagat tcgtcaggca
     1321 ctggcgcagg cgaaccgcgt cgaacatcag cgggccgggc gccacgccct ccacccggaa
     1381 gatcagctcg atgcccttgt cgcgcgccgt ggtctgatag ccgccgatga ggcgggcgca
     1441 gacctggacc agatcgccgg ccgttggggc gatttccagc ttgccggcct cgatcttgga
     1501 caggtccaga atgtcgttga gcagcaccag cagggtgtcg ccggagtcca ggatcagatc
     1561 gacctgctcg cgctgatgat cgcccagtgc ttcggagcgc agagactggg ccagacccag
     1621 catgccgttc aaaggggtgc gcagttcatg actcatcacc gccaggaagg ccgacttggc
     1681 ctggttggcg gcctcggcct tggcccgtgc gtcgcgcagc gagcgggaga tccggcgctg
     1741 gttgaacgcc cagctcactc cgcccagcac cagcagggcc aggagcaccg cgatcacccc
     1801 ggaggcggcg gccaggcggc ggttcagctc gacttcagcc gccagccggc ggctttcgtc
     1861 gcgcttggcg ctcagctcgc tctccagcgc cgccgacatt tcagcaacgc tgctcgaatg
     1921 ggccctgtcg ttttcggcgc ggtccacgcg tcgccaacga tccagcttct ggaaggcgga
     1981 ggcgccgcgt ccctcggcct ggtcgatata ggcctcaacc aggagttc
//
LOCUS       DAOMEO010000605         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5817_length_2026_cov_2.569254, whole genome shotgun sequence.
ACCESSION   DAOMEO010000605 DAOMEO010000000
VERSION     DAOMEO010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_2026_cov_2.569254"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..368)
                     /gene="clpS"
                     /locus_tag="PLH31_12900"
     CDS             complement(39..368)
                     /gene="clpS"
                     /locus_tag="PLH31_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HQR90130.1"
                     /translation="MAERKQGGQNNGTGSSVITEVKPKTQKPSLYRVLILNDDYTPME
                     FVVYVLERFFNKSREDATRIMLLVHQHGVGVCGVYTYEVAETKVAQVIDSARRHQHPL
                     QCTMEKD"
     gene            complement(603..1859)
                     /locus_tag="PLH31_12905"
     CDS             complement(603..1859)
                     /locus_tag="PLH31_12905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQR90131.1"
                     /translation="MKALCRDCDWTGEVKTPRCPSCGSPRTIFHEELASLSMAHLDCD
                     AFYASVEKRDDPSIRDKPVIVGGGKRGVVTTACYIARMSGARSAMPMYKALKLCPDAV
                     VIKPDFAKYKAASRHIMGKLEALTPLVQPLSLDEAWIDLSGTERLHGAPPAVMLTRVQ
                     REIEAEIELTVSIGLAPNKFLAKIASELDKPRGFSAIGAAEAKAFLAGKPVSILPGVG
                     PATVSALASEGFRTVGDIAAADLKHLAKVLGSSGLRLHRLAQGQDARAVDPDQIRKTI
                     SAETTFNDDLFKREDLEDQLWPLCERVARQARVEGVAGRVATLKLRTPDFKVHTRRRT
                     LAVPTQTARTLFQVARELLAAEPGGTYYRLIGAGLTEFVPAEGAADDFFAGDERRALK
                     SETAIDALRGKYGSGSVVTGRALKGR"
     gene            complement(1869..>2026)
                     /locus_tag="PLH31_12910"
     CDS             complement(1869..>2026)
                     /locus_tag="PLH31_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR90132.1"
                     /translation="MSNVEALYERLPAAMAPHVFSDTFETPVRTAMHGDSSGVRGAVW
                     LWPPQDA"
BASE COUNT          324 a          624 c          728 g          350 t
ORIGIN      
        1 cgagagggcg agaaaacgag ggcaaggggg gaggctcctc agtctttttc catggtgcac
       61 tgcagggggt gctgatgacg gcgcgccgag tcgatgacct gcgcaacctt cgtttcagcg
      121 acttcgtagg tatagacgcc gcacacacca acaccgtgtt ggtgaacgag cagcatgatc
      181 cgagtcgcgt cttcgcgtga cttgttgaag aaccgttcga ggacgtacac gacgaattcc
      241 atcggcgtgt agtcgtcgtt cagaatcagt actcgataga gcgaaggctt ctgtgtcttc
      301 ggtttcacct cagtgatgac cgaagatcca gttccgttat tctgaccgcc ttgcttccgc
      361 tcggccattg accgttttct cccggcgggc cgaaccaccc gccttgttag gattagatat
      421 ggaaacgcgc gggctttgga aggggccgac gcgatagtgc gccgctttaa cacaggggcg
      481 ccctgcaaaa tggcttcacg attgggggag cggggccgtg agatgacggt ttcccggggc
      541 gaggagccga aatggggcgc gtcgcatcgt cgcgcaaggg gtagtggtcg cgcggatccg
      601 actcaccgtc ccttgagggc ccggccggtg accacggagc cggagccgta tttgccgcgc
      661 agggcgtcga tggccgtctc gcttttcagc gcccggcgct cgtcgccggc gaagaagtcg
      721 tcggccgcgc cctcggcggg gacgaactcg gtcaggccgg cgccgatcag gcggtagtag
      781 gttccgcccg gctcggccgc caggagctcg cgcgccacct ggaacagggt acgagccgtc
      841 tgggtgggca ccgccagggt gcggcggcgg gtgtgaacct tgaagtcggg ggtgcgcagc
      901 ttcagtgtcg ccacgcgtcc ggccacgccc tcgacgcgcg cttgcctggc gactcgctcg
      961 cacaacggcc acagctggtc ttccagatcc tcgcgcttga agaggtcgtc gttgaacgtg
     1021 gtctcggcgc tgatcgtctt gcggatctgg tcgggatcga cggcgcgggc gtcctggccc
     1081 tgggccagac gatgcaggcg caggccgctg gagcccagga ccttggccag atgcttcagg
     1141 tcggccgcgg cgatgtcgcc gacggtgcgg aagccttccg aggccagggc cgagacggtg
     1201 gcggggccga cgccgggcag gatcgagacc ggcttgccgg ccagaaaggc cttggcttcc
     1261 gccgcgccga tggcggagaa gccgcgcggc ttgtccagtt cagaggcgat cttggctagg
     1321 aacttgttgg gggccaggcc gatcgagacg gtcagctcga tctcggcctc gatctcgcgc
     1381 tgaacccggg tcagcatcac ggcgggcggc gcgccgtgca ggcgctcggt gccggaaagg
     1441 tcgatccagg cttcgtccag cgacagcggc tgcaccaagg gcgtgagggc ctccagcttg
     1501 cccatgatat ggcggctggc ggccttgtac ttggcgaagt cgggtttgat gaccacggcg
     1561 tcggggcaca gcttcagcgc cttgtacatc ggcatggccg agcgggcgcc gctcatgcgg
     1621 gcgatgtagc aggcggtggt gaccaccccg cgcttgccgc cgccgacgat caccggcttg
     1681 tcgcggatcg agggatcgtc gcgcttctcg accgaggcgt agaaggcgtc gcagtccaga
     1741 tgggccatcg acagcgaggc cagctcttcg tggaagatcg tgcggggcga gccacacgac
     1801 gggcagcgcg gggtcttgac ctcgcccgtc cagtcgcagt cgcggcacag ggccttcatg
     1861 tgaggcccct aggcgtcttg gggtggccac agccagaccg cgccgcgcac gccggagctg
     1921 tcgccatgca tcgccgtgcg caccggggtc tcgaacgtgt cggaaaagac gtgcggcgcc
     1981 atggcggcgg gcaggcgctc ataaagcgcc tcgacattcg acatgc
//
LOCUS       DAOMEO010000606         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5818_length_2026_cov_1.893455, whole genome shotgun sequence.
ACCESSION   DAOMEO010000606 DAOMEO010000000
VERSION     DAOMEO010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_2026_cov_1.893455"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..394
                     /locus_tag="PLH31_12915"
     CDS             65..394
                     /locus_tag="PLH31_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HQR90133.1"
                     /translation="MAQRAEISFAAQNDRHSTYERITHIGGGVAKPWKLTIKEAIKLV
                     DSGKWRFFVMRDDVEVKVEAQTSRTGCRYLKTANDRNEPHDLLTLPGFPVTETETVEA
                     SESASAA"
     gene            483..>2026
                     /locus_tag="PLH31_12920"
     CDS             483..>2026
                     /locus_tag="PLH31_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90134.1"
                     /translation="MGDSYARCGAVAAIAVSVAMGLTMAPNPASAQSSSQRDIRVPFG
                     PPVSGDGYTGGSVGVSYAFLNCFGEIHISYSVAAGTAKTSDVYLMGGKTYIPDGVAPQ
                     PGSIRFAGTVSRGGEVIGSFADPYAATYSAKGCISGQTQKVGMMTPWVAKGATPDQII
                     NFLNSLSLRAEPAGPVRDAALESRLKAQDRARQAAVENQQRQEKAAAEARRLAEERRK
                     AEAAKPVSRTPSGPGGGYASQQASPSPQSAPRPLTDAERGDRAIASDRILAQQRLAEE
                     QRIYHQRQLAAAEAERQRQIQMNAAMVQAAPIIYGALESVTNSIDQAFAQAKANRWSA
                     AQSNLIGKCRDGAEGNWPIAKDGDLVLGVPTRSELTVADCGDRPDNRFKTFRLMVDEP
                     MRVRIKADGSIWMNATIVLIGTKPQKTVITRYDAPATGVISASSAIVANLSPGEYHVM
                     IHNAAGAGFRPFTVLVQKVDAKNRVIRTEPPPPPLNTVEAFPSPPQAPTPGPAPTRAI
                     GGIAGV"
BASE COUNT          385 a          702 c          648 g          291 t
ORIGIN      
        1 gctttgcgcg caaccgcaaa ctgaccggcg ccgttgctta ccctgcgcgg acccggaaga
       61 taccatggct caacgcgccg aaatcagctt tgccgcccag aacgaccgtc acagcaccta
      121 tgagcggatc acccacatcg gcggcggcgt cgccaagccc tggaagctga cgatcaagga
      181 agccatcaag ctggtcgaca gcggcaagtg gcgcttcttc gtcatgcgcg acgatgtcga
      241 ggtcaaggtc gaggcccaga ccagccgcac cggttgccgc tatctgaaga cggccaacga
      301 ccgcaacgag ccccacgacc tgctcacgct tcccggtttt ccggtgacgg agaccgagac
      361 agtcgaagcc agcgaaagcg ccagcgcggc ctaagtccgg gcccacgaaa cgccgaaaac
      421 catgcggctt gtcggcggcg ggatgacctg actactctcc tcccacacgc ggggggaacc
      481 agatggggga tagttacgca cgctgcggcg cagttgctgc gatagcggtg tcggtggcca
      541 tgggcctgac gatggcgccg aacccggctt cggcgcaatc ctcgagccag cgtgacatcc
      601 gggtgccgtt cgggccgccg gtaagcggcg atggatatac cggcggttcg gtcggcgttt
      661 catacgcctt cctgaactgt tttggcgaaa tccacatcag ctacagcgtg gccgccggca
      721 cggcgaagac cagcgacgtc tatctgatgg gcggcaagac ctatatcccc gacggcgtcg
      781 cgccccagcc cgggtctatc cgctttgcgg gcacggtctc gcgcggcggc gaggtgatcg
      841 gcagtttcgc cgatccctat gccgcgacct acagcgccaa gggctgcatc tcgggccaga
      901 cccagaaggt ggggatgatg accccctggg tggccaaggg cgcgacgccc gatcagatca
      961 tcaactttct caacagcctg tcgctcaggg cggagcccgc tggacccgtt cgcgacgccg
     1021 ccctggaaag ccgcctgaag gcccaggatc gcgcccgcca ggccgccgtc gaaaatcagc
     1081 agcgccagga aaaggccgca gcagaggccc ggcgtctggc cgaagagcgg cggaaggccg
     1141 aagcggccaa gcctgtctcg cgcacgccct ccggccccgg cggcggttac gcgtcgcagc
     1201 aggcctcgcc ctcaccccag tccgcgccgc gcccgctcac cgacgccgag cggggtgaca
     1261 gggccatcgc gtccgacaga atcctggccc agcagcggct cgctgaggag caacggatct
     1321 atcaccagcg acagctggcg gccgccgagg cggagcgtca gcgccagatc cagatgaacg
     1381 ccgccatggt ccaggccgcg ccgatcatct atggcgcgtt ggagagcgtg accaacagca
     1441 tcgaccaggc ctttgcccag gcaaaagcca atcggtggag cgccgcccaa tccaatctga
     1501 tcggaaaatg ccgggacggg gcggaaggca actggccaat cgccaaggac ggcgacctgg
     1561 tgctgggcgt gccgacgcgc tcggagttga ccgtcgccga ctgcggcgac cgacccgaca
     1621 atcgcttcaa gaccttccga ttgatggtcg acgagccgat gcgcgtacgg atcaaggccg
     1681 atggttcgat ctggatgaac gccaccatcg tgctgatcgg gacgaagcct cagaaaaccg
     1741 tcatcacgcg ctatgatgcg ccggccaccg gcgtcatctc ggccagcagc gccatcgtcg
     1801 ccaatctgtc gcccggcgag taccacgtga tgatccacaa tgcggccggg gcaggtttca
     1861 ggccgttcac cgtgctggtc cagaaggtcg acgccaagaa ccgggtcatt cgcaccgagc
     1921 cgcccccacc gccgctcaac acggtcgagg ctttcccgtc gccgcctcag gctccgacgc
     1981 ccggccccgc gccgacccgt gcgatcggcg gcatcgccgg tgtgtt
//
LOCUS       DAOMEO010000607         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5837_length_2019_cov_2.836049, whole genome shotgun sequence.
ACCESSION   DAOMEO010000607 DAOMEO010000000
VERSION     DAOMEO010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_2019_cov_2.836049"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..>1197)
                     /locus_tag="PLH31_12925"
     CDS             complement(56..>1197)
                     /locus_tag="PLH31_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR90135.1"
                     /translation="RAGLYYVCVSTKLTAAETQYILQDCGAKVFITSTAMGPIIDEIP
                     ALVPGLKLYAVGGAHGPYADFEAARAAMPETPISDESAGSDMLYSSGTTGRPKGVKPA
                     LSEGPIDAPNACGQFVPTHFVRMLKLPEETRAKYDVSSMKSAVHAAAPCPVPVKEQMI
                     AWWGPVIFEYYAGTEGNGFCWINSDNWLTHKGSVGQAVLGELRICDEDGNPVPPRTEG
                     TVYFANGPAVNYHNAPDKTAESYNQHGWTTLGDVGWADEEGYLYLTDRKSFMIISGGV
                     NIYPQEIENLLITHPKVADAAVVGAPCDEMGEKVVAVIQPLAWAEDHSDLADELMAFA
                     RANLSHVKSPRRIDFMQELPRHATGKLYKRLIRDAYWAEGGKRIG"
     assembly_gap    1198..1207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1208..1387)
                     /locus_tag="PLH31_12930"
     CDS             complement(<1208..1387)
                     /locus_tag="PLH31_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR90136.1"
                     /translation="MHPYLHAQTQGQKAAYIMAGSGETVTYAQLDARSNQGAQLFRSL
                     GLKAGDVIAILMENNA"
     gene            1456..1755
                     /locus_tag="PLH31_12935"
     CDS             1456..1755
                     /locus_tag="PLH31_12935"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQR90137.1"
                     /translation="MALSIKTEEADRLARDLSRLTGETMTQAVTVALRERLERERAAK
                     AETTEEFVARIRAFTAEMRGGVPFRPVTPEEWLDAGGDDLDFELAAKELAAKAKD"
     gene            1755..>2019
                     /locus_tag="PLH31_12940"
     CDS             1755..>2019
                     /locus_tag="PLH31_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR90138.1"
                     /translation="MVIDTSAIIAIIADEPTADDLTVRIARAAVPEMSVVSYVEAATV
                     LLARNISPDRLDTLLIRLGISVVDVDVVQGRAAVRARIAYGKGT"
BASE COUNT          335 a          629 c          663 g          382 t
ORIGIN      
        1 atccttgcca ccacttccaa caatgtggtg gcaaggatta tgaaaatcaa cccggctacc
       61 cgatccgctt tccgccctcg gcccagtacg cgtcccggat cagccgcttg tagagcttgc
      121 ccgtcgcgtg gcggggcagc tcctgcatga agtcgatccg gcgcggcgac ttcacatggc
      181 tgagattggc gcgggcgaag gccatcagtt cgtcggcgag atcagagtgg tcctcggccc
      241 aggccagagg ctggatcacc gccaccacct tctcgcccat ctcatcgcac ggcgcgccga
      301 ccacggcggc gtcggccacc ttggggtggg tgatcagcag gttctcgatc tcctgcggat
      361 agatgttcac cccgccgctg atgatcatga agctcttgcg gtcagtgagg tacagatagc
      421 cttcctcgtc ggcccagccc acgtcgccca gcgtcgtcca gccgtgctgg ttatagctct
      481 cggcggtctt gtcgggggcg ttgtgatagt tcaccgccgg gccgttggcg aaatagaccg
      541 tcccctcggt gcggggcgga accgggttgc cgtcctcgtc gcagatgcgc agctcgccca
      601 gcacggcctg gccgacgcta cccttgtgag tcagccagtt gtcggaattg atccagcaga
      661 agccgttgcc ttccgtgccc gcataatatt cgaagatcac cgggccccac caggcgatca
      721 tctgttcctt gaccggcacc gggcagggcg cggcggcgtg gacggccgat ttcatcgacg
      781 agacgtcgta tttggcgcgc gtctcctcgg gcagtttcag catccgcacg aagtgggtcg
      841 gcacgaactg gccgcaggcg ttgggtgcgt cgatcgggcc ctcgctcaag gctggcttga
      901 cgcccttggg ccgccccgtg gtgcccgagg aatacagcat gtccgagccc gcgctctcgt
      961 cgctgatcgg cgtttccggc atggccgcgc gcgccgcctc aaagtcggca taggggccat
     1021 gcgcaccacc caccgcgtaa agcttcaggc ccgggaccag ggcggggatc tcgtcgatga
     1081 tcggtcccat ggccgtcgag gtgatgaaca ccttggcccc gcagtcctgc aggatgtact
     1141 gcgtctccgc ggccgtcagc ttggtcgaga cgcacacata gtagagcccg gctcgctnnn
     1201 nnnnnnncgc gttgttttcc atcaggatgg cgatgacatc gccggccttc aggcccagcg
     1261 agcggaacag ctgcgcgccc tggttcgatc gcgcgtccag ctgggcatag gtgaccgtct
     1321 cgcctgagcc ggccatgatg taggcggcct tctggccttg agtctgagcg tggagatagg
     1381 ggtgcatgac ggcggacctt ggatgacttt tggggcggat tgatctagat tgcttgtcta
     1441 gaaacggagg cgatgatggc gctcagcatc aagactgaag aggccgaccg tctggcccgc
     1501 gacctctccc gcctgaccgg cgagacgatg acccaggccg tgaccgtggc cctgcgcgaa
     1561 cggctggagc gcgagcgggc ggccaaggcg gaaacgaccg aggagttcgt cgcgcggatc
     1621 agggcgttta cggccgaaat gcgtggcggc gtccccttcc ggccagtcac gcccgaggaa
     1681 tggttggacg ctggcggcga cgatttggac tttgagctgg cggccaagga gctggcggcc
     1741 aaggccaagg actgatggtg atcgacacct cggcgattat cgccatcatc gcggatgaac
     1801 caacggctga cgacttgacg gttcgtatcg ctagggccgc agtcccggaa atgtccgtcg
     1861 tcagttatgt cgaagccgcc acggttctct tggcgagaaa catatcgccg gaccgtctcg
     1921 acaccttgct tatccggctg ggcatatcgg ttgtcgatgt ggatgtggtc caaggtcgcg
     1981 cagctgttcg cgccaggatc gcgtacggca aggggacgg
//
LOCUS       DAOMEO010000608         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5847_length_2018_cov_2.049414, whole genome shotgun sequence.
ACCESSION   DAOMEO010000608 DAOMEO010000000
VERSION     DAOMEO010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_2018_cov_2.049414"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1835)
                     /locus_tag="PLH31_12945"
     CDS             complement(231..1835)
                     /locus_tag="PLH31_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HQR90139.1"
                     /translation="MNTTAGPATGGVLTRRRFFEGLAAVGGISLALSGMDALGFGMAS
                     ATELPPTLTGDAKGTRILILGAGLAGMTAAYELSKAGYQVQILEARSFAGGRCQTSRK
                     GFEHTDLLGNKQTCEFDDGLYINHGAWRIPYHHRSTLHYTKLFGVLLESFVNDNDAAY
                     VFFEKGEGPLKGKPIRKGEIAADARGYAAELIAKAASKGELDAALTPADREKFVAYMI
                     NEGRLSPKDLAYTGTEGRGFSVHPGAGTSPGPGKELPPFAFKDVLDSGAWRVLSSVTG
                     YEQQRTMLQPIGGMDQIAKGFEKQVGAMIRYSTVVEKIKQTDKGVKVAFKEASGKTGE
                     IEADYCICTIPLSVLKQIDLDASAPFKAAMAGVSYAPVNKIGLQMKTRFWEDKHHIYG
                     GHIYNDIPGIGTITLPSTGWQSQKGVLLGFYNFGGQAARISAMKPAERAAFAVAGGQK
                     VFPEYAENFENAFSFCWHLAEFNLGGWAEWGEDGRRDAYPLLCEPDGRLYLAGEHLSY
                     LGGWQAGAIESAWQQIAKIHARVQQV"
BASE COUNT          336 a          657 c          668 g          357 t
ORIGIN      
        1 ccggtcacct gatggcaggc cgagcaattg tcgtcataca ggctcttggg cgacgcggcc
       61 gaggcgacgg cgggcgctga cgcggcggcg acgaggcaga gcagggccag gcgggaagca
      121 aacttcatcg ggtcctcgga tccttgagct ctcctgacga ggagagccct acatctatcg
      181 tgttgagcgt gacgggcgcc gacagctgtg tccagcgtcg gcggccacgc ttagacctgc
      241 tgcacgcggg cgtggatctt ggcgatctgc tgccaggccg actcgatggc cccggcctgc
      301 cagccgccaa ggtagctgag gtgctcgccg gccagataca ggcggccatc gggctcgcac
      361 agcaaaggat aggcgtcgcg ccgaccgtct tcgccccatt cggcccagcc ccccagattg
      421 aactcggcca ggtgccagca gaacgagaag gcgttctcga aattctcggc gtattcgggg
      481 aacaccttct ggccgccggc cacggcgaag gccgcgcgtt cggccggctt catggcgctg
      541 atgcgcgccg cttggccgcc gaaattgtag aagcccagca acacaccctt ctggctctgc
      601 cagccggtgg agggcagggt gatcgtgccg atgccgggaa tgtcgttata gatgtggccg
      661 ccgtagatgt ggtgcttgtc ttcccagaac cgggtcttca tctgcaggcc gatcttgttg
      721 accggcgcat aggacacgcc tgccatcgcc gccttgaagg gggccgaggc gtccagatcg
      781 atctgcttga gcacgctgag cgggatcgtg cagatgcagt agtcggcctc gatctcgccg
      841 gtcttgcccg aggcctcctt gaaggcgacc ttcacgccct tgtcggtctg cttgatcttc
      901 tcgaccacgg tcgagtagcg gatcatcgcg ccgacctgct tttcgaagcc cttggcgatc
      961 tggtccatgc caccgatcgg ctgcagcatg gtgcgctgct gctcgtagcc cgtgaccgag
     1021 ctcagaaccc gccacgcgcc gctgtcgagc acgtccttga aggcgaaggg cggtagttcc
     1081 ttgcccggac cagggcttgt tcccgcgccg ggatgcacgg aaaaaccgcg cccctcggtg
     1141 cccgtatagg ccaggtcctt gggcgacagg cggccctcgt tgatcatgta ggcgacgaat
     1201 ttctcgcgat cggccggggt cagggcggcg tcgagttcgc ccttgctggc ggccttggcg
     1261 atcagctcgg cggcatagcc gcgcgcgtcg gcggcgatct cgcccttgcg gatcggcttg
     1321 cccttcagcg ggccctcgcc cttctcgaaa aagacatagg cggcgtcatt gtcgttgacg
     1381 aagctttcca gcagcacgcc gaacagcttg gtgtagtgca gggtcgagcg gtggtgatac
     1441 gggatccgcc aggcgccgtg gttgatgtag aggccgtcgt cgaattcgca ggtctgcttg
     1501 ttgcccagca ggtcggtgtg ctcgaagccc ttgcgcgagg tctggcagcg accgccggcg
     1561 aaagaccggg cttccaggat ctgcacctga tagccggcct tggagagctc ataggcggcg
     1621 gtcatgcccg ccaggcccgc gcccaggata aggatcctgg tccccttggc gtcgccggtc
     1681 agggtgggcg gcagttcggt ggccgaagcc atgccaaagc ccagggcgtc catccccgac
     1741 agcgccaggg aaatgccgcc caccgcggcc agaccctcga agaaacgccg ccgtgtcaga
     1801 accccacccg ttgccgggcc tgcagtcgta ttcatgccaa tccgccactc agaaacagcc
     1861 gacgccgaaa acgcgtgcat cgccgaaacg atacaccctt cgttgctccg gtcgaacgcg
     1921 cccccgccga agtcacgggc atctcgaaat gtcggcggaa cgggcgcaac ccgatttcgg
     1981 gcggcggccg atctgacatg tcagatgcgc gccggatg
//
LOCUS       DAOMEO010000609         2015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5856_length_2015_cov_2.043367, whole genome shotgun sequence.
ACCESSION   DAOMEO010000609 DAOMEO010000000
VERSION     DAOMEO010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_2015_cov_2.043367"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..540
                     /locus_tag="PLH31_12950"
     CDS             163..540
                     /locus_tag="PLH31_12950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQR90140.1"
                     /translation="MNAFLLQYLPIVIFLGIATIIGVAFLAAAAVLAPRAPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVSILFIIFDLEVAFLFPWAVSLMKLPHEAAVFAFWSM
                     MTFLGVLTVGFIYEWKKGALEWE"
     gene            540..669
                     /locus_tag="PLH31_12955"
                     /pseudo
     CDS             540..669
                     /locus_tag="PLH31_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286864.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
     assembly_gap    670..679
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <680..1062
                     /gene="nuoB"
                     /locus_tag="PLH31_12960"
     CDS             <680..1062
                     /gene="nuoB"
                     /locus_tag="PLH31_12960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HQR90141.1"
                     /translation="FGLACCAVEMMQASMPRYDLERYGFAPRASPRQSDVMIVAGTLT
                     NKMAPALRKVYDQMPEPRYVISMGSCANGGGYYYYSYSVVRGCDRVVPVDIYVPGCPP
                     TAEALVYGVLQLQKKIRRTGTIER"
     gene            1059..1658
                     /locus_tag="PLH31_12965"
     CDS             1059..1658
                     /locus_tag="PLH31_12965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQR90142.1"
                     /translation="MSEALELLGQDIVARAPGVLGHSVAFGELTIVARAATVCDTLVF
                     LRDDAACRFHQLIDLAGVDYPERAARFDVVYHLLSLVKNHRIRLKVSTDEDTPVPTIT
                     PVFEVANWFEREAFDMYGILFEGHPDLRRILTDYGFHGHPLRKDFPMTGYVEVRYDDE
                     LKRVVYEPVKITEFRAFDFLSPWEGAKYALPGDEKAEAK"
     gene            1658..>2015
                     /locus_tag="PLH31_12970"
     CDS             1658..>2015
                     /locus_tag="PLH31_12970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522355.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQR90143.1"
                     /translation="MADDASIVPAIPETPVRKFNINFGPQHPAAHGVLRLVLELDGEV
                     VERVDPHIGLLHRGTEKLMEARTYLQNIPYFDRLDYVAPMNQEHAYCLAIEKLLGVDV
                     PLRGQIIRVLYSEIGRI"
BASE COUNT          342 a          607 c          621 g          435 t
ORIGIN      
        1 cggcgagggg cgtctgatat gtcggtcgcg gactcgtgtc aacggccatg tcgtcgaagc
       61 cgcagatgag gattagttgc gatcgtttct caatgtgatg caggtgtttg aagcggtggc
      121 ggcagtgggc tacaacccgc tcgattcaag ccaagggctg tcatgaacgc cttccttctc
      181 cagtacctgc cgatcgtcat cttcctcggg atcgcgacga ttatcggggt cgccttcctg
      241 gccgccgccg ctgtgctcgc gccaagggcg ccggaccctg aaaagctctc cgcctacgaa
      301 tgcggcttca acgctttcga cgatgcgcgc atgaagttcg atgtccggtt ctatctggtc
      361 tcgatcctgt tcatcatctt cgacctggaa gtggcgttcc tgtttccctg ggccgtcagt
      421 ctgatgaagc tgccgcatga agcggcggtc ttcgcgttct ggtcgatgat gaccttcctg
      481 ggcgtgctta ccgtcggctt tatctacgaa tggaagaagg gcgccctcga atgggagtga
      541 tcgttcccgc caatagctcg cccctcgtcc cggctggctc cgctggccgt tcgacggtgc
      601 agggctatga tcccaagctg cacgatccgt tcttcgacgg catctcgggt cagttggccg
      661 acaagggctn nnnnnnnnnc gttcggtctg gcgtgctgcg ccgtggaaat gatgcaggcc
      721 tcgatgccgc gctatgacct ggagcgctat ggcttcgcgc cacgcgccag tccgcgtcag
      781 agcgacgtga tgatcgtggc cggcaccctg accaacaaga tggctccggc tctgcgcaag
      841 gtctacgacc agatgcccga gccgcgctat gtcatctcga tgggcagctg cgccaatggc
      901 ggcggctatt actattacag ctacagcgtc gttcgcggct gcgaccgcgt ggtgccggtg
      961 gacatctatg ttccgggttg cccgccgacc gccgaggccc tggtctatgg cgtcctgcag
     1021 cttcagaaga agatccgtcg cacggggacg atcgagcgat gagcgaagcc ctcgaacttc
     1081 tcggtcagga tattgttgcc cgcgcgcccg gcgtgctggg tcattcggtg gcgtttggcg
     1141 aactgaccat cgtggctcgt gccgcgacgg tctgtgacac gctagtcttc ctgcgtgacg
     1201 atgcggcttg ccgttttcac cagctgatcg atctggctgg cgtggactac ccggagcgcg
     1261 ccgcgcggtt cgacgtggtc tatcacctgc tgtcgctggt gaagaaccat cggattcgcc
     1321 tgaaggtctc gaccgacgaa gacacgccgg ttccgacgat cacgccggtg ttcgaagtcg
     1381 ccaactggtt cgagcgagaa gcgttcgaca tgtatggcat cttgtttgaa ggtcatccgg
     1441 acctgcgccg aatcctgacc gactatggct tccacggcca tccgctgcgg aaggacttcc
     1501 cgatgacggg ttatgtggaa gttcgctatg acgacgagct gaagcgcgtc gtctatgagc
     1561 cggtcaagat caccgagttc cgcgcctttg acttcctttc cccatgggaa ggcgccaagt
     1621 acgccctgcc gggcgatgaa aaagcggagg cgaagtaatg gctgacgacg cttctatcgt
     1681 gcctgcgatc ccggaaaccc cggttcgcaa gttcaatatc aatttcggcc cgcaacaccc
     1741 ggccgcgcac ggtgtgctgc gcctggtgct ggagctggac ggcgaagtcg ttgagcgggt
     1801 cgatccgcat atcggcctgc tgcatcgcgg caccgaaaag ctgatggagg cgcgcaccta
     1861 cctccagaac atcccttact tcgaccgcct cgactatgtg gcgccgatga accaggagca
     1921 tgcctactgc ctggccattg aaaagctgct tggcgtggac gtcccgcttc gggggcagat
     1981 catccgtgtg ctctattcgg aaatcggccg gatcc
//
LOCUS       DAOMEO010000610         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5857_length_2014_cov_3.021440, whole genome shotgun sequence.
ACCESSION   DAOMEO010000610 DAOMEO010000000
VERSION     DAOMEO010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_2014_cov_3.021440"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLH31_12975"
     CDS             complement(<1..747)
                     /locus_tag="PLH31_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQR90144.1"
                     /translation="MNRDYTRRASMRLVLVMVSSLVLTACGDGGKPTAETEGHAAEAG
                     DYERGPHRGRMLRNGDLAIEITIFEDGVEPEFHVYAYRKDKPLDPREVQLSVELTRLG
                     GKVDRFSFTPQEDYLRGAGVVTEPHSFDVKVRASQGGQSHQWTYPSYEGRTTISAQAA
                     RAGGVKTEIAGGADVAELVDMAGRVEITPEGKGEVRAWYPGRVMTLRGELGTQVRKGQ
                     LLARVESSESLQTYSIFAPISGIIVEKNINV"
     gene            complement(744..1214)
                     /locus_tag="PLH31_12980"
     CDS             complement(744..1214)
                     /locus_tag="PLH31_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR90145.1"
                     /translation="MQDPTWRAGLRYLNEGRDMAVVVGGSIPLARYDTNRGAIERALA
                     ERTAADADLLSGKVVRERQIARLQADLAARASEARRIEAEVLPAAERTVALVREGFNR
                     GGFSYLDVIEAQKVLIDARSRRLDALKAYHTHRAQLARLTGRHAALIPASETVR"
     gene            complement(1117..1941)
                     /locus_tag="PLH31_12985"
     CDS             complement(1117..1941)
                     /locus_tag="PLH31_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR90146.1"
                     /translation="MSRPIHGRLTRSWLLASAFAVALPIVSLPLVAQAQSLALDQALA
                     RAAQADPTRPAVEARLKAADAGARQAGVRANPVIGVDLEDLTGTGPYSLVDRAQATVY
                     YQQSLERGGKREARTSLARIEIDLVRLRHETKVLDLFREVELAWVEAMAAEAQVRLAK
                     IRLDIAERAQGEVDRRVKAARDPLFAGARADAQVAEARIILSQAQMTADTTRRALADY
                     WNGGVARRRTADSSSAGSRGRNQGRAGPDLAGRPALSERRPRHGGRGRRINPPGAV"
BASE COUNT          307 a          669 c          688 g          350 t
ORIGIN      
        1 gacattgatg ttcttctcga cgatgatccc gctgatcggc gcgaagatcg aataggtctg
       61 aaggctctcg ctggactcga cgcgcgccag cagctggccc ttgcggacct gggttcccaa
      121 ctcgccgcgc agggtcatca cccgccccgg ataccaggcg cgaacctcgc ccttgccctc
      181 gggtgtgatc tccacacggc cagccatgtc caccagctcg gcgacatccg cgccgccggc
      241 gatctcggtc ttcacgcccc cggccctcgc ggcctgggcg ctgatcgtcg tgcggccttc
      301 gtaggacgga taggtccatt gatggctttg accaccctgg ctggcgcgca ccttgacgtc
      361 gaaggagtgc ggctccgtta cgacaccggc gccgcgcagg tagtcctcct gcggcgtgaa
      421 gctgaaccgg tcgaccttgc cgcccagacg ggtcagttcg accgacagct ggacttcgcg
      481 cggatcgagc ggcttgtcct tccgataggc atagacgtgg aactcaggct ctacgccgtc
      541 ctcgaaaatg gtgatctcga tggccaggtc gccgttgcgc aacatgcggc cacgatgcgg
      601 gccgcgctcg tagtcgccag cctcggcggc gtgtccttcg gtctcggctg taggcttgcc
      661 gccatcgccg caggccgtca gcaccaggct cgaaaccatc accaggacaa ggcgcatcga
      721 ggcgcgccgg gtgtaatcgc ggttcatcgg acagtctccg aagcgggaat aagggccgcg
      781 tggcggcctg tcaggcgagc cagctgggcg cggtgtgtgt ggtaggcctt cagggcgtct
      841 aggcgacggc ttcgcgcgtc gatcagtacc ttctgggcct cgatgacgtc gagatagctg
      901 aagccacccc ggttaaaacc ttcgcggacc agggcgacgg tgcgctcggc ggcgggcaac
      961 acctcggctt cgatccgacg agcttcgctg gcccgtgcgg ccaagtcggc ctgaagcctg
     1021 gcgatctgtc gctcgcgcac aaccttgccg ctgagcagat cggcgtcggc cgctgtgcgc
     1081 tctgccaggg cgcgttcgat ggcgccgcga ttggtgtcat accgcgccag ggggattgat
     1141 ccgccgacca cgaccgccat gtcgcggcct tcgttcagat agcgcaggcc ggcccgccag
     1201 gtcgggtcct gcacggcctt ggtttcttcc acgcgaaccc gcgctgctgc tgtctgccgt
     1261 tcggcgtcga gcaacgccgc cgttccagta atcggccagg gcccgccggg tagtatcggc
     1321 tgtcatctga gcttgcgaca gtatgatcct ggcctcggcg acctgggcgt cggcgcgggc
     1381 gccggcgaac agcgggtccc gcgccgcctt cacgcggcga tcgacttcgc cctgggcgcg
     1441 ctcggcgatg tccaagcgga ttttagccag gcgaacctgg gcttcagccg ccatggcttc
     1501 gacccaggcc agttcgacct ccctgaaaag gtccagaacc ttggtctcat gccgaagtcg
     1561 gacgagatcg atctcgatgc gcgccagcga cgtgcgggcc tcgcgcttgc cgccgcgctc
     1621 caggctctgc tggtagtaga cggtggcctg ggcgcgatcg acgagggaat agggaccggt
     1681 tcccgtcagg tcttccagat caacgccgat gacaggattg gcgcgaacgc cggcttgccg
     1741 ggcgcccgcg tcggccgcct tcaggcgagc ctctacagcc ggacgagtag ggtcggcctg
     1801 ggcggctcgg gcgagcgcct gatccaaagc cagggattgg gcttgcgcca cgagcggcag
     1861 gctgacgatg ggcagcgcga cggcgaatgc cgacgcgagc agccaggatc gcgtgaggcg
     1921 accgtggata ggacgggaca tggatttctc tcgaacggag gcaggcgcgg cgcagggccg
     1981 cgtcggtcag gcgacgtgtt cgaggctggc cttt
//
LOCUS       DAOMEO010000611         2013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5862_length_2013_cov_2.608274, whole genome shotgun sequence.
ACCESSION   DAOMEO010000611 DAOMEO010000000
VERSION     DAOMEO010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5862_length_2013_cov_2.608274"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLH31_12990"
     CDS             complement(<1..304)
                     /locus_tag="PLH31_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQR90147.1"
                     /translation="MIGPMNKIRAHSAAFATGVALLFGSTAMVAPAAAFAQAAQTGVV
                     QRIVVQGNERIEQGTVMSYLPIQPGDTVDAARLDLALKTLARTDLFADVKIEMVSGD"
     gene            complement(322..>1762)
                     /gene="rseP"
                     /locus_tag="PLH31_12995"
     CDS             complement(322..>1762)
                     /gene="rseP"
                     /locus_tag="PLH31_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQR90148.1"
                     /translation="PLVILVVVTVHELGHFWAAKACNVEIERFSIGFGKAIVSWRDRA
                     GVQWQIGWLPLGGYVRFAGDENAASVPDKEDLASMRTAIERREGREAVNRYLHFKPLW
                     QRAFIVAAGPLANFVLAITLFAVLLGVFGETVRRPVISGVNAGSPAAAAGFRQGDVII
                     SADGRKVKNFGELDNYALLRSDVPIRFEIERGGKSLELVATPALVEISDGFGGSQKAG
                     VLGFGAPPLIASVEPGSPAEKAGLQRGDVIQRADGAAIGSFEDLTRHVTQRGGKPIAF
                     EVYRGGETINIVATPTLLTRPDADGKPKTRMLLGVAGGLPASDIQRVRYNPIEAIAGG
                     FARTWEVLETTVYYLGRVIRLEVSAEQISGPIGIAKTSGQLAQAGAAGATDLPSMLLG
                     SAVALIWLSAILSVSVGFMNLLPIPVLDGGHLLFYAYEAIARRPLGARLQAAGYRVGL
                     ALLLSFMLFATWNDLQRLSVFKFFGGLFS"
     assembly_gap    1763..1772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1800..>2013)
                     /locus_tag="PLH31_13000"
     CDS             complement(1800..>2013)
                     /locus_tag="PLH31_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQR90149.1"
                     /translation="AANEVAVAAFLDRQIGFLDIAASVAGTLERMNSLGALMEADTDP
                     VESAMTTDASARRIAAEVVRQVHRHN"
BASE COUNT          394 a          661 c          628 g          320 t
ORIGIN      
        1 ggtcgcccga caccatctcg atcttgacgt ctgcgaacag gtccgtgcgc gccagggtct
       61 tgagcgccag gtcgaggcgc gcggcgtcaa cggtgtcgcc cggctggatc ggcaggtagg
      121 acatcaccgt gccctgctcg atccgctcgt taccttgcac gacgattcgc tgaacgacgc
      181 cggtctgcgc cgcctgggca aaggccgccg caggcgcgac catcgcggtc gaaccgaaga
      241 gcagcgccac accagtggcg aacgcggcgc tatgggcgcg aattttgttc atgggaccaa
      301 tcatggacgg aaggcgaccg ctcaagagaa cagcccgccg aaaaatttga atacgctgag
      361 gcgctgcaga tcattccagg tggcgaacaa catgaaactc agcaacagcg caagaccaac
      421 cctgtacccg gccgcctgaa gccgggcgcc aagcggccgc cgggcgatcg cttcgtaggc
      481 gtagaacagc aggtgtccgc catccaaaac agggatcggc aacaggttca tgaaaccgac
      541 gctgaccgag aggatggccg agagccagat cagcgcgacc gctgaaccca gcagcatcga
      601 gggaaggtcc gtggcgcccg ccgcgcctgc ctgagccaac tgaccggagg tcttggcgat
      661 gccgatggga ccgctgatct gttcggcgga gacttccaga cgaatgaccc gacccagata
      721 gtaaaccgtc gtttcgagca cttcccaggt ccgggcaaag ccgcctgcga tggcctcaat
      781 gggattgtag cgcacgcgct gaatgtcgct ggcgggcagg ccgccggcta cgcccagcag
      841 catgcgagtc ttgggtttgc cgtcggcgtc cggacgggtc agcagagtcg gcgtggcgac
      901 gatgttgatg gtttcaccgc cccgatagac ctcaaaggcg atcggcttgc cgccccgctg
      961 ggtcacatga cgcgtcaggt cttcaaagct gccgatcgca gcgccatcgg cgcgctggat
     1021 gacatcccca cgctgaaggc cagccttttc ggcgggcgag cccggctcga cgctggcgat
     1081 caggggcggc gcgccgaaac ccagaacccc ggccttctgg ctgccgccaa agccgtccga
     1141 gatctccacg agcgcgggcg tcgccaccag ttcgagcgac ttgccgccgc gctcgatctc
     1201 gaagcggatc ggaacatcgc tgcgcagcag cgcataattg tccagttcgc cgaagttctt
     1261 caccttgcgg ccatcggccg aaatgatcac gtcgccctgc cggaagccgg ccgcagcggc
     1321 cggactaccc gcgtttacgc cgctgatgac cggtcgacgc acggtctcgc caaacacgcc
     1381 cagcagaacc gcaaacagcg tgatcgccag gacgaaattg gccaaggggc ccgccgcgac
     1441 gataaaggcg cgctgccaaa ggggtttgaa atgcaggtac cgattgaccg cttcgcgacc
     1501 ctcgcgacgc tcgatcgcgg tgcgcatact ggccagatct tccttgtcgg gaacgctggc
     1561 cgcgttctca tcgccggcga agcgaacata gccgcccaag ggtagccatc cgatctgcca
     1621 ctgcacgccg gcgcggtccc tccacgagac gatggccttg ccaaaaccga tcgagaatcg
     1681 ctcgatctcc acgttgcagg ccttggcggc ccagaagtgt cccagctcgt gaactgtcac
     1741 gaccaccagg atcacgaggg ggnnnnnnnn nnccgagcat gtcggatctc ttctcgtctt
     1801 cagttgtggc gatgaacttg gcgtacaacc tcggccgcaa tgcggcgggc gcttgcatcc
     1861 gtcgtcatgg cgctttcgac cggatcggtg tcggcttcca tcaaggcgcc cagactgttc
     1921 atgcgttcaa gggtccccgc caccgaggcg gcaatatcga gaaagccgat ctgacggtca
     1981 aggaacgccg ccacggccac ttcattggcg gcg
//
LOCUS       DAOMEO010000612         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5877_length_2010_cov_2.985166, whole genome shotgun sequence.
ACCESSION   DAOMEO010000612 DAOMEO010000000
VERSION     DAOMEO010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5877_length_2010_cov_2.985166"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>2010)
                     /locus_tag="PLH31_13005"
     CDS             complement(62..>2010)
                     /locus_tag="PLH31_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90150.1"
                     /translation="GEGFDLPELKIAALHDHHKSIAVTIQFVGRFTRQDPRLGDATVI
                     ANTGVDDVDRSLAKLYAEDADWNALVEALSSAKIERQIRRAEMFKGFTGDLNDIPLQT
                     LEPKMNAVVYRTSCESWDPFQAEELYDAGSYLGMKINPHQRVAIFVTRAEEQARWTTA
                     QHAINVTWDLHMLHWDQASGLLYISSSAKGPFDRLAKAVCGDTARRIEGEDVFRSLHG
                     FKRLILRNLGLTHHQGRGVRYSMYMGLDSAKSQSRIKNNVFGTGFLDGAPASRGCSAK
                     GKFWSMSAVRDLTDWVDWCQDIGRAVNDPGITTDGVFKSAMRPREINQRPAVPPVAIH
                     WPESLLKQFEERIEVTFGEHAVPFAECDIELLDNARSGPLRFAVRSDDHSAEFEIVFG
                     DGTRYPQRAGPKATIRVAGKVQTLSDSFGDDAPHIDFGDGSLLIHSHLFALPEGESVE
                     PYPAEKLEVWDWSKTNIRAEAQGPEKRADSVQRLVIETLLADPEPYDVIFDDDGKGEI
                     ADVVALRMTDSVVSVTLFHCKYSGTDTPGARVGDLYEVCGQAQKSARWRDRPNWMLKH
                     MLKREQIRRDKGLSSRIERGSAAMIKKLKAGWQDHRFEYDVRIVQPGLSRQAIGEEGL
                     HLIAGVETYLLETRAMRLRVIGSA"
BASE COUNT          337 a          656 c          625 g          392 t
ORIGIN      
        1 acatcggccc ccgcgtctct ctcttcgggc gcccggcgcc aatgttgaac caaggcgcgt
       61 ctcacgcgct gccgatgacc cgcaggcgca tggcgcgggt ctccagcaga taggtctcga
      121 cccctgcgat caggtgaagc ccctcctcgc caatggcttg gcgggaaagc cccggctgca
      181 caatccgcac atcgtactcg aaccgatggt cctgccagcc ggccttgagc ttcttgatca
      241 tcgcggccga gccgcgctcg atccgggagc tgaggccctt gtcgcgccgg atttgctcgc
      301 gcttcagcat gtgctttagc atccagttcg gccggtcgcg ccagcgcgcc gacttctggg
      361 cctgcccgca cacttcgtag aggtcaccga cccgggcgcc gggcgtatcg gtccccgaat
      421 atttgcaatg gaacagggtc actgacacga cgctgtcggt catgcggagc gccaccacat
      481 cggcgatctc gcccttgcca tcgtcgtcga agatcacgtc atagggttcg gggtcggcga
      541 gcaatgtctc gatcaccagc cgctggaccg agtcggcccg cttctccgga ccttgcgctt
      601 cggcacggat attggtcttc gaccagtccc acacctcgag cttctccgcc ggatagggct
      661 ccacgctttc gccttcgggc aatgcgaaaa gatggctgtg gatcagtagc gaaccgtcgc
      721 cgaaatcgat gtgcggcgcg tcgtctccga aggaatccga gagcgtttga actttgccgg
      781 cgaccctgat cgtggcctta gggccggccc gctgcggata gcgcgtgcca tcaccaaaga
      841 cgatttcgaa ctccgccgaa tggtcgtcgc tccgcactgc gaaccgcaac ggtccgctgc
      901 gtgcattgtc gagcagctcg atatcgcatt cagcgaaggg cacggcgtgc tcgccgaagg
      961 tgacttctat gcgctcttcg aattgcttca gaagcgattc aggccagtgg atcgccaccg
     1021 gcgggacggc ggggcgctgg ttgatttcac gcggccgcat cgcgctcttg aacaccccgt
     1081 cggtagtgat gccgggatcg ttcaccgcgc ggccgatatc ctgacaccaa tcgacccagt
     1141 cggtgagatc gcgcaccgcc gacatcgacc agaacttccc cttcgccgag caacccctcg
     1201 acgccggcgc accgtcgagg aagccggtgc cgaaaacatt gttcttgatc cgcgattgtg
     1261 acttggcgct gtccaggccc atgtacatgg aatagcgcac gccgcgcccc tggtgatggg
     1321 tcaggccaag gttgcgcagg atcaggcgct tgaagccgtg caagctgcgg aagacatcct
     1381 cgccctcgat ccgccgcgcc gtgtcgccgc acaccgcttt ggccaggcga tcgaaggggc
     1441 ctttcgccga gctgctgatg tagagcaggc cgctggcctg gtcccaatgc agcatgtgca
     1501 ggtcccaggt gacgttgatg gcgtgctgcg ccgtcgtcca acgcgcctgt tcctccgcgc
     1561 gggtgacgaa gatcgccacg cgctggtgcg gattgatctt catgccgagg taggagccgg
     1621 cgtcgtagag ttcctcggcc tgaaacggat cccagctctc acacgaggtc cgatagacca
     1681 ccgcgttcat cttcggctcg agggtctgca agggaatgtc gttgaggtcg ccggtgaagc
     1741 ccttgaacat ctcggcgcgt cggatttggc gctcgatctt tgccgagctc agggcctcga
     1801 ccagcgcgtt ccagtcggcg tcctcggcgt agagcttggc cagcgagcgg tcgacgtcgt
     1861 cgactccggt gttggcgatg accgtcgcgt cgcccaacct tgggtcttgt cgggtgaagc
     1921 ggccgacgaa ctgaatggta accgcaatgc ttttgtgatg gtcgtgcagg gccgcaatct
     1981 tcagctcagg gaggtcgaat ccttcgccca
//
LOCUS       DAOMEO010000613         2006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5892_length_2006_cov_2.079959, whole genome shotgun sequence.
ACCESSION   DAOMEO010000613 DAOMEO010000000
VERSION     DAOMEO010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_2006_cov_2.079959"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLH31_13010"
     CDS             complement(<1..559)
                     /locus_tag="PLH31_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQR90151.1"
                     /translation="MSVQDRIRVRETKLLSDNWYVLKTTRFDWKRSDGTWQTQAREHY
                     DRGNGAVLLPYNLAAKTVLLVRQFRYPAFINGYDDLLIEAAAGLLDDAEPEARIRAEV
                     EEELGYRLGAVRKVFEAFMSPGSVTEILHFFVAEYNSAMRIGAGGGHPDEGEDIEVLE
                     LSIDEALAMIADGRIRDAKTIMLLQH"
     gene            complement(568..1686)
                     /gene="zapE"
                     /locus_tag="PLH31_13015"
     CDS             complement(568..1686)
                     /gene="zapE"
                     /locus_tag="PLH31_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HQR90152.1"
                     /translation="MPTSLRTAYRERLAQGEIKPDAAQAAAVEALSRLEAELDSAGEP
                     SFSLFRRKPKSQRGVYLWGPVGRGKSMLMDLFFDSAPVTRKRRIHFHAFMAEVHAHID
                     AWRKGDAAERRARFGQHKGDDPIAPTAELISSQARLLCFDELQVTDIADAMILGRLFE
                     GLFAHGVTLVATSNRPPDDLYKDGLNRQLFVPFIDMLKSALDVVAVRGAVDFRLDRLR
                     AARTWLSPNDKASQAEFERLWADMLDGAAETGANLEVLGRKIRLPRAAGGMLRSSFAS
                     LCQQALGPQDYLALAERFHTVFLEDVPRLTPERRDAAKRFNTLIDALYEADAKLVALA
                     DAEPESLYPAGDGAFEFERTVSRLQEMRSADYVARVRD"
     gene            complement(1748..>2006)
                     /locus_tag="PLH31_13020"
     CDS             complement(1748..>2006)
                     /locus_tag="PLH31_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90153.1"
                     /translation="WKGDPAIGYAALGAWSFSAPLADCQGAVAMVERRGAVDGVQIRN
                     IIAPDRGRILVVFSDDAEFDFGEIWQGRGPSYTLASEAFCR"
BASE COUNT          329 a          670 c          681 g          326 t
ORIGIN      
        1 agtgctgcag cagcataatg gtcttggcgt cgcggatgcg gccgtcggcg atcatggcca
       61 gggcctcgtc gatgctgagc tccagcacct cgatgtcctc gccctcatca gggtggccgc
      121 cgcccgcgcc gatccgcatg gcgctgttat actcggccac gaagaagtgc aggatctcgg
      181 tcaccgagcc ggggctcatg aaggcttcga acaccttgcg aactgcgccc aggcgatagc
      241 ccagctcttc ctcgacctcg gcgcggatcc gcgcttcggg ctcggcgtcg tcgagcaggc
      301 cggcggcggc ttcaatcagc aggtcatcat aaccgttgat gaaggccggg tagcggaact
      361 gccgaaccaa caggaccgtc ttcgccgcca gattgtaggg cagcagcacc gcgccgttgc
      421 cgcggtcata gtgctcgcga gcctgggtct gccaggtccc gtcgctgcgc ttccagtcga
      481 atcgggtcgt cttcaggacg taccagttgt cggacagcag tttggtctcg cgcacccgga
      541 tgcggtcctg aacgctcatg gcgtctccta atcccgcacc cgggcgacat agtcggccga
      601 gcgcatttcc tgcagacggg aaaccgtccg ctcaaactcg aacgcgccgt cccccgccgg
      661 gtacagggac tcgggctccg cgtcagccag agccaccagc ttggcgtcgg cttcgtagag
      721 cgcgtcgatc agggtgttga aacgcttggc cgcatcacgc cgctcaggcg tcaggcgcgg
      781 cacgtcttcc agaaagacgg tgtggaagcg ctcggccagg gccagatagt cctggggtcc
      841 cagggcctgc tggcacaggc tggcgaagga cgatcgcagc atccctccgg cggcgcgcgg
      901 caggcggatc ttgcggccca gcacctcaag gttggcgccg gtctcggccg cgccatccag
      961 catgtcggcc cagagacgct cgaactcggc ctggctggcc ttgtcgttgg gcgacagcca
     1021 ggtgcgggcg gcgcgcagcc ggtcgaggcg aaagtccacc gcccctcgca cggccaccac
     1081 gtccagcgcg cttttcagca tgtcgataaa ggggacgaac agctggcgat tcaggccgtc
     1141 cttgtagagg tcgtccggcg ggcggttgct ggtcgccacc agggtcaccc catgggcgaa
     1201 gagcccttcg aacaaacggc ccaggatcat ggcgtcggcg atgtcggtga cctgcagctc
     1261 gtcgaagcac aacaggcgcg cctggcttga aatcaactcg gcggtgggcg ctatgggatc
     1321 gtcgcccttg tgctggccaa accttgccct gcgctcggcg gcgtcgccct tgcgccaggc
     1381 gtcgatatgg gcgtgcacct cggccatgaa ggcgtgaaaa tggatccgtc gcttgcgggt
     1441 gacgggggcg ctgtcgaaga acaggtccat cagcatggac ttgccgcgcc ccaccggccc
     1501 ccacagatag acgccgcgct ggctcttggg cttgcggcga aacagcgaaa agctgggctc
     1561 gccggccgag tccagctcag cctccagccg cgacagggcc tcgacggcgg cggcctgggc
     1621 ggcatcgggc ttgatctcgc cctgggccag gcgttcgcga taggcggtgc ggagtgaagt
     1681 gggcatcgag actgcggtta gcgcgggagc ggcgccaggg aaagggcctg tctgcggtgg
     1741 gtcgggttca gcgacagaac gcttcggacg ccaacgtgta gctgggcccc cgcccctgcc
     1801 agatttctcc gaagtcaaac tcggcgtcgt cgctgaagac caccaggatc cgcccgcgat
     1861 ccggggcgat gatgttgcgg atctggacgc catcgacggc gccacgccgc tcgaccatcg
     1921 cgaccgcgcc ctggcagtcc gccaggggcg cgctgaaaga ccaggccccc agggcggcgt
     1981 agccgatcgc cgggtcacct ttccac
//
LOCUS       DAOMEO010000614         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5897_length_2005_cov_2.948718, whole genome shotgun sequence.
ACCESSION   DAOMEO010000614 DAOMEO010000000
VERSION     DAOMEO010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_2005_cov_2.948718"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLH31_13025"
     CDS             complement(<1..342)
                     /locus_tag="PLH31_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HQR90154.1"
                     /translation="MKRVWFGAAALAALSLSAFSAQASEDHLCLDDACTTQALFAPAI
                     EGAAADLTSLESPRYGAWGFDTSGIDSSVKPGDDFYKWANGAWDARTEIPSDRTRYGN
                     FDKLSELSEART"
     gene            complement(439..924)
                     /locus_tag="PLH31_13030"
     CDS             complement(439..924)
                     /locus_tag="PLH31_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQR90155.1"
                     /translation="MRTARFLALAAVLAFTAPAMAGDYKVGALTIKSPWSRPAMAGMN
                     GVGFMTIVNTGAKPVKLVSAESPLAGKVEIHRSAMANGVMTMRRQDDGVIIPAGGATS
                     FAPGGYHLMLMGLKKPLTQGQKAPVTLVFDNGRRAAVELTVQIAAPQATQGSAGGHAH
                     H"
     gene            complement(939..1442)
                     /locus_tag="PLH31_13035"
     CDS             complement(939..1442)
                     /locus_tag="PLH31_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HQR90156.1"
                     /translation="MRKIVLTLLALAAPLPAAAHVVAAPDTARAGSYAAVAFRVGHAC
                     SAGDVTLKVRIEIPPGVVSARPQPKPGWTYEIEREAGADPKAAPSAVTFTGRLPDEAF
                     DDFPLLMKLPTAADTLVFPVIQTCEKGESQWAEVPSTGAPGEKLSRPAATLRLIPADA
                     ASAGHKH"
     assembly_gap    1513..1522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1523..1985)
                     /locus_tag="PLH31_13040"
     CDS             complement(<1523..1985)
                     /locus_tag="PLH31_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90157.1"
                     /translation="MVDPAHPAATFNADETVHQGLEAGLDWRFNKRVRLRQTYAWSDF
                     HFEGDAQYGDNSLPIVPEHLYRAELKYEHPAGWFVAPAVEWSPRGPFVDYRNKVRAPD
                     YAIASLGAGYTRDGVTVFLDARNLLDKRYVATVNPLANSASVTAATIENTAA"
BASE COUNT          318 a          613 c          729 g          335 t
ORIGIN      
        1 ggtgcgagct tccgacagtt ccgacagctt gtcgaaattg ccatagcggg tgcggtccga
       61 cgggatctcg gtgcgggcgt cccaggcgcc attggcccac ttgtagaagt cgtcgcccgg
      121 cttgacgctg ctgtcgatgc ccgaggtgtc gaagccccag gcgccgtagc gcggcgactc
      181 cagcgaggtc aggtcggccg ccgcgccttc aatggcgggg gcaaacagcg cctgggtcgt
      241 gcaggcgtca tcaaggcaca gatggtcttc gctggcctgg gcgctgaagg ccgagaggga
      301 gagggcggcc aaggcggcgg cgccgaacca aacacgtttc atggaagtcg tatccgttgg
      361 tgtgagctgg ccgcaatctg cctcgccgtg aaaattttgc tcccttacaa atttgtcatg
      421 tgtggctgag gccggcggtc agtgatgggc gtgcccgccc gccgatcctt gcgtggcctg
      481 gggcgcggcg atctggaccg tcagctccac ggccgcacgg cggccattgt cgaagaccag
      541 tgttacggga gctttttgcc cttgggtcag gggcttcttc aggcccatca gcatcaggtg
      601 atagccgccc ggggcgaagg atgtggcccc gccggccggg atgatcacgc cgtcgtcctg
      661 gcggcgcatg gtcatcacgc cattggccat ggccgagcgg tggatctcaa ccttgccggc
      721 cagcgggctt tcggcgctga ccagcttcac cggcttggcc ccggtgttga cgatggtcat
      781 gaagccgacg ccgttcatgc cggccatggc cgggcggctc catgggcttt tgatcgtcag
      841 ggcgccgacc ttgtagtcgc cggccatggc aggggcggtg aaggccagga cggccgcgag
      901 ggccaggaag cgtgcagtgc gcatggggct ctccaggatc aatgtttgtg gcccgccgac
      961 gcagcgtcgg cgggtatcag gcgaagggtg gcggcggggc gcgacagttt ctcgccgggc
     1021 gcgccggtcg agggaacctc cgcccactga ctttcaccct tctcgcaggt ctggatgacc
     1081 ggaaagacca gggtgtcggc ggcggtgggc agcttcatca gcagcgggaa gtcgtcgaac
     1141 gcctcgtcgg gcagacggcc ggtgaaggtg accgccgagg gcgcggcctt gggatcggcg
     1201 ccggcctcgc gctcgatctc ataggtccag ccgggcttgg gctggggcct ggccgagacc
     1261 acgccgggcg ggatctcgat ccggaccttc agagtgacgt cgcccgccga gcaggcatgg
     1321 cccacccgga aggcgacggc ggcgtaggag ccggcgcggg cggtgtccgg cgcggccacg
     1381 acatgagcgg cagcgggcag gggagcggcc agggccagca gggtgaggac gatcttgcgc
     1441 atcagggcct cctagaactt gcccgagacg ccgacgaagg acaacagaca atgccgggtc
     1501 cattcgatca agnnnnnnnn nnaggcggcc gtgttctcga tggtcgccgc tgtcaccgag
     1561 gccgaattgg ccagcggatt gacggtggcg acatagcgct tgtccagcag attgcgcgca
     1621 tccaggaaca ccgtcacccc gtcgcgggta tagcccgccc ctaaggatgc aatcgcgtag
     1681 tcgggcgcgc ggaccttgtt gcgatagtcc acgaacgggc cgcgcggcga ccattccacc
     1741 gccggggcca cgaaccagcc ggccgggtgc tcgtatttca gctcggcgcg atacaggtgc
     1801 tcaggtacga tcggcaggct gttgtcgcca tactgggcgt cgccctcgaa gtggaagtcc
     1861 gaccacgcat aggtctggcg caggcgaacg cgtttgttga aacgccagtc gagtccagct
     1921 tcgaggccct ggtggaccgt ttcgtcggcg ttgaaggtgg cggccgggtg ggcgggatcg
     1981 accacgaagg tcagcagctc gtcct
//
LOCUS       DAOMEO010000615         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5899_length_2005_cov_2.386154, whole genome shotgun sequence.
ACCESSION   DAOMEO010000615 DAOMEO010000000
VERSION     DAOMEO010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_2005_cov_2.386154"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PLH31_13045"
     CDS             complement(<1..1482)
                     /locus_tag="PLH31_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90158.1"
                     /translation="MSRRVVFSAVSLLALACAMGAHANEAAPAQDDAGVSKVVVTAAP
                     YAVSADNLTASVAIVDRTDLDLAPPTGLGDLLSGMPGVRSTAFGAGASRPVVRGLAGP
                     RVQVLTNGVGQIDASALSPDHQVATDPGEAERIEVLRGPAALAYGGSAIGGVINIIDD
                     RIASRAPDGGYSGRLLASGATGDDSYAVSGAIHAAVGPLVLTLDGLKREADDYEIPVF
                     AESRRLLASEGETAEGGGGKLANTAVELEAIGAGVSYVGADGFIGVSVKKTDTSYGVP
                     GHAHEEEGAEEEGPVTIGLTQTRIDLRGEYNKAIGPFAKVRLSAGHADYKHTEFEGDA
                     VGTRFMSDGYEGRVELVQAERGGWKGAVGVQGLRRNFDAQGDEAYVPRTKITEYGAFT
                     QQRVEKAGWGYEGGLRIDYRDLDSLKGQADFTNVSGSLGVFARPSDAVFFGLAVSRNS
                     RAPTEAELYAEGPHVATRGFEIGNPNLDAETAWSVEATAHYSGE"
     gene            1629..1771
                     /locus_tag="PLH31_13050"
                     /pseudo
     CDS             1629..1771
                     /locus_tag="PLH31_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519005.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1807..>2005)
                     /locus_tag="PLH31_13055"
     CDS             complement(1807..>2005)
                     /locus_tag="PLH31_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90159.1"
                     /translation="TGVNTLAAPVLDHRGDLVSELGRDDEGLVAASFDLDFLQTHRAA
                     WGFFRDRRSDLYGALASPRPA"
BASE COUNT          326 a          684 c          692 g          303 t
ORIGIN      
        1 ctcgccggaa tagtgggcgg tggcctcgac cgaccaggcg gtttccgcgt cgaggttcgg
       61 gttgccgatc tcgaagccgc gtgtggccac gtgcgggccc tcggcataga gctcggcctc
      121 ggtcggggcg cggctgttgc gcgacaccgc cagaccaaag aacaccgcgt ccgacggccg
      181 ggcaaagacg cccagcgaac cggagacgtt ggtgaaatcg gcctggccct tcaggctgtc
      241 caggtcgcga tagtcgatcc gcagaccgcc ttcatagccc cagccggcct tttcgacccg
      301 ctgctgggtg aaggcgccgt attcagtgat cttcgtcctg ggcacatagg cctcgtcgcc
      361 ctgggcgtcg aagttgcggc gcaggccctg gacgcccacc gcgcccttcc agccgccgcg
      421 ctcggcctgg accagctcga cccggccctc atagccgtcg gacatgaacc gggtgccgac
      481 cgcgtcgccc tcaaactcgg tgtgcttgta gtcggcatgg ccggccgaca ggcggacctt
      541 ggcgaagggg ccgatagcct tgttgtactc accgcgcagg tcgatgcggg tctgggtcag
      601 gccgatggtg accggaccct cctcttcggc gccctcttct tcatgggcat ggccgggcac
      661 accgtaggat gtatcggtct tcttgaccga gacgccgatg aagccgtcgg cgcccacata
      721 ggacacgccc gccccgatgg cctccagctc gaccgccgta ttggccagct tgccgccgcc
      781 gccctcggcc gtctcgccct cggaggccag caggcgccgg gattcggcga agaccggaat
      841 ttcatagtca tcggcctcgc gcttcagacc gtccagggtc aggaccagag ggcccacggc
      901 cgcatggatc gcgccggaga ccgcatagct gtcgtcgccc gtcgcgcccg aggccagcag
      961 ccggccgcta tagccgccgt ccggggcgcg gctggcgatg cggtcgtcga tgatgttgat
     1021 caccccgccg atggccgagc cgccataggc cagggccgcc gggccgcgca gcacctcgat
     1081 gcgctcggcc tcgccgggat cggtggccac ctggtggtcg ggcgacaggg cgctggcgtc
     1141 gatctggccg acgccattgg tcagcacctg cacgcgcgga ccggccaggc cccgcaccac
     1201 cgggcggctg gcccccgcgc caaaggccgt cgagcgcacg ccgggcatgc cgctgagcag
     1261 atcgcccaga ccggtcggcg gcgcgagatc gaggtcagtg cgatcgacga tggcgacgct
     1321 ggccgtcagg ttgtcggccg acaccgcata gggcgcggcg gtgaccacca ccttggagac
     1381 cccggcgtca tcctgggccg gagcggcctc attggcgtgg gcgcccatgg cgcaggccag
     1441 ggccagcagg ctgacagcgg agaaaacgac gcgacgcgac atgacgaaag tcctgaaccg
     1501 tggccggggg agcggccttg ttcaggagtg ttatgaaata acatatcgac tcgtcaagcg
     1561 cctttgcgcc tggccgagac taggcgggat cgacattcag agcttcggga tgctcaagcc
     1621 caaattatat agcgccgtca ttcccgccct tgtggcggga acccctctgt ccgccgcagg
     1681 tgcgggaggg gcgaagtgcg cgcgcacttc gctggcgctt cacgtgtcag ctgaaacagg
     1741 ggttcccgcc acaagggcgg gaatgacggg aaaaatggga aggatgggct gaatgtcgat
     1801 cagaccttaa gccggacgcg ggctggccag cgccccatac agatccgagc gccgatcccg
     1861 gaagaagccc caggccgcgc ggtgggtctg caggaagtcg agatcgaacg aggccgccac
     1921 caggccctcg tcgtcgcgcc ccagttcgct gaccagatcg ccgcgatggt cgagcacggg
     1981 ggccgccagc gtattgacgc cggtg
//
LOCUS       DAOMEO010000616         2004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5902_length_2004_cov_2.941508, whole genome shotgun sequence.
ACCESSION   DAOMEO010000616 DAOMEO010000000
VERSION     DAOMEO010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_2004_cov_2.941508"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2004
                     /gene="ptsP"
                     /locus_tag="PLH31_13060"
     CDS             <1..>2004
                     /gene="ptsP"
                     /locus_tag="PLH31_13060"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQR90160.1"
                     /translation="RGPRSLLRQIREAMAGAGPAQGKLDMVVRTIAISMVAEVCSIYL
                     RRASGDLELFATEGLAPEAVHVTRLKPGEGLVGETMRIGRPLNLSDAPNHPAFAYRPE
                     TGEDPYHAFLAVPLLRGGRSIGVLVVQNRTERIYGEEEVEDLQIIAMVLAEMVSSSEL
                     LGLDELKDVEIAPHKPERLKGARFAEGLAYGVAVLHEQPVAPEQLLSDDSAAEEIRLK
                     VAVTALQSQIDEMLEGQGGLVGASYEVLDTYRLFAHDRGWNRGLEEAVRSGLTAEAAV
                     ERVRSEHRARLGQARDPYLRERLHDLEDLNDRLLRHLSGDVHAVRQLPDDAILIARNL
                     GPADLLEYDRTKLKGILLEEGSAASHAGIVARALDIPCVGRLAGLRDRVSEGDPVVVD
                     AETAEAWLRPRPDVVKALKARMEVRAQRKAEFARLRDTPAFTKDGSKITLLMNAGLAV
                     DLDILGETGAEGIGLFRTEFQFMVAEEMPRLEAQTALYEKVLEAANGMPVTFRTLDLG
                     GDKLLPYMEMEREDNPALGWRAIRMGLDRPALLRMQIRALIKASRGRELRIMFPLVAS
                     VDEFRAARAFVDQEVAWALKRGRPAPARLDVGAMIEAPSLLWHLDALLPMTDFVSVGT
                     NDLMQYLFAADRGNPRMADRYDPLSPAALRALKTIQQACADTGT"
BASE COUNT          313 a          692 c          692 g          307 t
ORIGIN      
        1 tcgaggacca cgcagtctgc ttcggcagat acgcgaggcc atggccgggg cgggccccgc
       61 ccagggcaag ctcgacatgg tggtgcgcac catcgccatc tcgatggtcg ccgaagtctg
      121 ctcgatctat ctgcgccgcg cttccggcga tctggaactg ttcgccaccg agggcctggc
      181 tcccgaggcc gtccacgtca cccggctgaa acccggcgag ggtctggtcg gcgagaccat
      241 gcgcatcggc cggccgctga acctgtccga cgccccgaac cacccggcct tcgcctatcg
      301 cccggaaacc ggcgaagatc cgtaccacgc cttcctggcc gtccccctgc tgcgcggcgg
      361 tcgctcgatc ggcgtgctgg tcgtccagaa ccgcaccgag cggatctatg gcgaggaaga
      421 ggtcgaagac ctccagatca tcgccatggt cctggccgag atggtcagct ccagcgagct
      481 tctgggtctg gacgagctga aggacgtcga gatcgcgccc cacaagccgg agcggctgaa
      541 gggcgcgcgc ttcgccgagg gtctggccta tggcgtggcc gtgctgcacg aacagccggt
      601 ggcgcccgag caactgctgt cggacgactc ggctgccgaa gagatccgcc tgaaggtcgc
      661 cgtgaccgcc ctgcagagcc agatcgacga gatgctggag ggccagggcg gtctggtggg
      721 cgcctcgtac gaggtgctcg acacctaccg cctgtttgcc catgaccgcg gctggaaccg
      781 cgggctggaa gaggccgtgc gctcgggtct gaccgccgag gccgctgtcg aaagggtgcg
      841 ctccgagcac cgggcccgcc tgggccaggc ccgcgatccc tatctgcgcg agcgcctgca
      901 cgatctggaa gatctcaacg accggctgct gcgccacctg tcgggcgacg tccatgccgt
      961 acgccagctg cccgacgacg ccatcctgat cgcgcggaac ctgggccccg ccgatctgct
     1021 ggaatacgac cgcaccaagc tgaagggcat cctgctcgag gaaggctcgg ccgccagcca
     1081 cgccggcatc gtggcccggg cgctggacat cccttgcgtg ggccgtctgg ccggcctgcg
     1141 cgaccgggtc agtgagggcg acccggtggt ggtcgacgcc gagacggccg aggcctggct
     1201 tcggccgcgg ccggacgtgg tcaaggcgct gaaggcccgg atggaagtcc gcgcccagcg
     1261 caaggccgag ttcgcccgtc tgcgcgacac gcccgccttc accaaggacg gctcaaagat
     1321 caccctgctg atgaacgccg gcctggccgt cgatctggac atcctgggcg agaccggggc
     1381 cgagggcatc ggcctgttcc gcacagagtt ccagttcatg gtcgccgagg agatgccgcg
     1441 cctcgaagcc cagacggcgc tctacgaaaa ggtgctggaa gccgccaacg gcatgccggt
     1501 gaccttccgt acgctcgatc tcggcggcga caagctgctg ccgtacatgg agatggagcg
     1561 tgaggacaat ccggccctgg gctggcgcgc catccgcatg ggtctggacc ggccggccct
     1621 gctgcggatg cagatccgcg ccctgatcaa ggcctcgcgg ggtcgcgagc tgcggatcat
     1681 gttccctttg gtggccagcg tggacgagtt tcgcgccgcc cgcgcctttg tggatcagga
     1741 agtcgcctgg gccctgaagc gcggccgccc ggcgccggcc cgactggacg ttggcgcgat
     1801 gatcgaggcg ccgtcgctgc tgtggcatct ggacgccctg ctgccgatga ccgacttcgt
     1861 ctcggtgggc acgaacgatc tgatgcagta tctgttcgcc gctgaccggg gcaacccgcg
     1921 catggctgac cgctatgatc cgctgtcgcc agcggccctg cgagcgctga agaccatcca
     1981 gcaggcctgc gcggataccg gaac
//
LOCUS       DAOMEO010000617         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5918_length_2002_cov_2.196713, whole genome shotgun sequence.
ACCESSION   DAOMEO010000617 DAOMEO010000000
VERSION     DAOMEO010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5918_length_2002_cov_2.196713"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLH31_13065"
     CDS             complement(<1..588)
                     /locus_tag="PLH31_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR90161.1"
                     /translation="MPSLGDNAPESGLEGGRKRATINDIARLAGVSKKTVSRVINQSP
                     FVRDETRERIEAVIAEWGYAPDPQARGLAFRRSFLIGMIYDNPNPQYVVNMQLGLLDG
                     MRGSGFELVVHPCNRASPTFLADVRTFVERQKLYGVVLPPSVSEDDRVAKLLNEIGCA
                     YIRIASVELDKPENMIVGHDRMGGAQAARHILDLGH"
     gene            770..>2002
                     /locus_tag="PLH31_13070"
     CDS             770..>2002
                     /locus_tag="PLH31_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HQR90162.1"
                     /translation="MTQPSSVRVESIHDVDPRDHVATALRDIVAGETLSLRGRSVLAR
                     ADIPKGHKIAIQGAAAGQDVLKYGWPIGRATIDIAVGDHVHVHNVETKLSGVEDYVWT
                     PSALAPVAAPTDRAFMGYRRRNGRVGTRNEIWVLCTVGCVANTARRIAEKANARFAGR
                     TDGVFAFPHPFGCSQLGDDLSHTRKLIAALAGHPNAGGVLILGLGCENNQLKTLLDSA
                     PDLDPARLRAFTTQMVDDELEDGLAAIEALIALAERDVREPCPVSDLVVGLKCGGSDG
                     FSGVTANPLVGRIADKVSDAGGTPVLTEIPEIFGAEQMLLARAASQTVFDSAVRVVDD
                     FKRYFIDNNQPIHENPSPGNIAGGITTLEEKSLGAVQKGGRSALVEVIRYGERVGPHG
                     LTLLEAPGNDAVSSTALTA"
BASE COUNT          349 a          669 c          643 g          341 t
ORIGIN      
        1 gtggcccaga tcgaggatat gccgcgccgc ctgggcgccg cccatgcggt catggcccac
       61 gatcatgttc tcgggcttgt cgagctcgac cgaggcgatg cggatatagg cgcaaccgat
      121 ctcgttgagc agcttggcga cgcgatcgtc ctccgaaacc gacggcggca gcaccacgcc
      181 gtacagcttc tggcgctcaa cgaaggtgcg aacgtcggcc aggaacgtgg ggctggcgcg
      241 attgcagggg tgcacgacca gctcgaagcc cgaaccgcgc atgccgtcca gcaggcccaa
      301 ctgcatgttc acgacatact ggggattggg attgtcgtag atcatgccga tcaggaacga
      361 gcgccggaac gccagccccc gcgcctgcgg atcgggcgca tagccccatt ctgcgatgac
      421 cgcctcgatc cgttcgcggg tctcgtcgcg cacgaagggg gactggttga tcacacgcga
      481 aacggtcttc ttcgatacgc cggccagccg ggcgatatcg ttgatcgtgg cgcgcttgcg
      541 tccaccctcc aggccgctct cgggtgcgtt gtcgcccagc gacggcatag gcggttttga
      601 agaattcacg gcgtttccat ggagctttga gcgctttctt ttgtcatact ctacacacgc
      661 gggcgtcgcc accaatggcc gaaacggacg tttgcaagaa aactgacacc ggttaccctg
      721 ccgtcgaacg acacttggcg gcttagaagg tcgccacacg aaggcacaaa tgacccaacc
      781 gagctctgtt cgcgtcgaat cgattcacga cgtcgatcct cgcgaccatg ttgcgaccgc
      841 cctgcgcgac atcgtcgccg gcgagacgct gagcctgcgc ggtcggtcgg ttctggctcg
      901 ggccgacatc cccaaaggcc acaagatcgc cattcagggc gcggccgccg gccaggatgt
      961 gctgaagtac ggctggccca tcggacgggc gacgatcgac atcgccgtcg gtgatcacgt
     1021 ccacgtccac aatgtcgaga ccaagctttc cggcgtcgaa gactatgtct ggacgccctc
     1081 ggcgctcgcg ccggtcgccg cgcccaccga tcgcgccttc atgggttatc gtcgccgcaa
     1141 tggcagggtg ggcacccgca acgagatctg ggtgctatgc actgtgggct gcgtcgccaa
     1201 caccgcccgc cggatcgccg agaaggcaaa tgcccgtttc gcgggccgca cggacggcgt
     1261 cttcgccttc ccccaccctt tcggttgctc gcagctgggc gatgacctgt cgcatacgcg
     1321 caagctcatc gccgccctcg ccggccatcc gaacgccggc ggcgtgttga tcctgggcct
     1381 gggctgcgag aacaaccagc tgaagaccct gctggacagc gcgccggatc tggatcccgc
     1441 ccgcctgcgc gcgttcacca cccagatggt cgatgacgaa cttgaagacg gcctggccgc
     1501 gatcgaggcg ctgatcgccc tcgccgaaag ggacgtccgg gaaccctgcc cggtctccga
     1561 tctcgtcgtg ggcctgaagt gcggcggttc tgacggtttt tcgggtgtga ccgccaaccc
     1621 gctggtgggt cggatcgccg acaaggtctc ggacgcggga ggcacaccgg tgctgaccga
     1681 gatcccagaa atcttcggcg ccgagcagat gctgctggcc cgcgccgcca gccagacggt
     1741 gttcgacagc gccgtgcggg tggtcgacga tttcaagcgc tacttcatcg acaacaacca
     1801 gccgatccac gagaacccct cccccggcaa tatcgccggc ggcatcacga ccctggagga
     1861 gaaatccctg ggcgcggtac agaagggcgg ccgctcggcc ctggtcgagg tgatccgcta
     1921 cggcgagcgg gtgggcccgc atggcctgac cctgctggag gcgcctggca atgatgcggt
     1981 gtcttcgacg gcgctgacag cg
//
LOCUS       DAOMEO010000618         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5930_length_1998_cov_2.409676, whole genome shotgun sequence.
ACCESSION   DAOMEO010000618 DAOMEO010000000
VERSION     DAOMEO010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5930_length_1998_cov_2.409676"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..676)
                     /gene="rpsE"
                     /locus_tag="PLH31_13075"
     CDS             complement(71..676)
                     /gene="rpsE"
                     /locus_tag="PLH31_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQR90163.1"
                     /translation="MARGDQQRGDGGPNRGRRDRNAPEERVDSDIVEKLVHINRVAAT
                     VKGGRRFSFAALMVVGDQKGRVGFGHGKAREVPEAIRKATEEAKKTMVRVPLRESRTL
                     HHDGYGRWGAGKVMMRAAPPGTGVIAGGPMRAVLETLGVQDVVAKSTGSSNPYNMVRA
                     TFEALKVQSSPRQIAAKRGKKVGDILGRRADGASAPDAIEG"
     gene            complement(690..1046)
                     /gene="rplR"
                     /locus_tag="PLH31_13080"
     CDS             complement(690..1046)
                     /gene="rplR"
                     /locus_tag="PLH31_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQR90164.1"
                     /translation="MALSPRESAVRRAQRTRTRLKALSNGRPRLTVFRSSKNIYAQII
                     DDERGVTLAAASTLEGEGKTKGADKDAAALVGKLVAERAIEKGVKDVVFDRGGYIFHG
                     RVKALADAAREAGLNF"
     gene            complement(1050..1583)
                     /gene="rplF"
                     /locus_tag="PLH31_13085"
     CDS             complement(1050..1583)
                     /gene="rplF"
                     /locus_tag="PLH31_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQR90165.1"
                     /translation="MSRIGKKAVAIPKGVTLTLEGQTVSVKGPKGQLSWTVPEDIEIK
                     VESEELTLTPRNESTRAKSMWGLSRTLVANMVHGVTTGFEETLELVGVGYRAAMKGTA
                     LSIQLGFSHDVDVEAPAGVTFAVPKQTEIKIAGADKQAVGEIAAKIRRIRPPEPYKGK
                     GVRYAGEKVRRKEGKKK"
     gene            complement(1602..>1998)
                     /gene="rpsH"
                     /locus_tag="PLH31_13090"
     CDS             complement(1602..>1998)
                     /gene="rpsH"
                     /locus_tag="PLH31_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQR90166.1"
                     /translation="SMNDPLSDLIARIKNAATRKRAKVATPASKLRARVLDVLADEGY
                     IRGYSLVEKPGAFPEFEIELKYFDGEPVIAEIARVSKPGRRVYSSIKDLKPIKNGLGI
                     SILSTPKGVMSDSAARDANVGGEVLCRVY"
BASE COUNT          315 a          626 c          662 g          395 t
ORIGIN      
        1 gctggacggc gttgcggcgg atcgggctgc cggtttggcg aaccttgacg gtttttgctt
       61 cagccatgtc ttagccctcg atggcgtccg gagccgaagc tccgtcggcg cggcggccga
      121 gaatgtcgcc aaccttcttg ccgcgcttgg cggcgatctg gcggggcgac gattgaacct
      181 tcagggcctc gaacgtggcg cgaaccatgt tgtacgggtt cgaagaaccc gtcgacttag
      241 ccacgacgtc ctggacgccc agggtttcga gaaccgcgcg catcggacca ccggcgatga
      301 cgcccgtgcc gggaggggca gcgcgcatca tgaccttgcc agcgccccaa cggccgtagc
      361 cgtcgtggtg cagggtgcgc gactcgcgga gcgggacgcg gaccatggtc ttcttggctt
      421 cttccgtggc cttgcggatg gcttcaggca cttcacgcgc cttgccatga ccgaagccga
      481 cgcggccctt ttggtcgcca acgaccatca gagcagcgaa gctgaagcga cggccgccct
      541 taacggtggc ggcgacgcgg ttgatgtgaa cgagcttttc gacgatgtcg ctgtcgaccc
      601 gctcttcggg ggcgttgcgg tcgcgacggc cacgattggg gccgccgtct ccgcgctgtt
      661 gatcaccacg agccatgctg tgatttccct tagaaattca ggccggcttc gcgcgcggcg
      721 tcggccaggg ctttcacccg accgtggaag atgtagccgc cgcgatcgaa gacgacgtcc
      781 ttgacgccct tttcgatggc gcgctcggcg acgagcttgc cgaccagtgc agccgcgtcc
      841 ttgtccgcgc ccttggtctt gccttcaccc tcgagggtgg aggcggcagc cagggtcacg
      901 ccgcgttcat catcgatgat ctgggcgtag atgttcttgg acgaacggaa gaccgtaaga
      961 cgcggacgac cgttggacag ggccttgagg cgggtgcggg tgcgttgggc acgacgcacg
     1021 gcggattcgc gaggtgagag cgccatggct tacttcttct tgccttcctt gcggcgaacc
     1081 ttctcgccgg catagcgcac gcctttgccc ttgtagggct cgggcggacg aatgcggcgg
     1141 atcttcgcgg caatctcgcc aaccgcctgc ttgtcagcgc cggcgatctt gatttcggtt
     1201 tgcttcggaa ccgcgaaggt gacgccggcc ggagcctcga catccacatc gtggctgaaa
     1261 ccgagctgga tggagagcgc agtgcccttc atcgcggcgc ggtaaccaac gccgaccagt
     1321 tcgagggttt cctcaaagcc ggtggtgacg ccgtgcacca tgttggcgac cagcgtgcgg
     1381 gacagacccc acatcgactt ggcgcgcgtg gactcgttcc gcggggtcag ggtcagctct
     1441 tcgctttcga ccttgatctc gatatcttcc ggaaccgtcc aggagagttg gcccttggga
     1501 cccttgaccg acacagtctg accctcgagc gtcagcgtga cgcccttggg gattgcgacg
     1561 gctttcttac cgatacgtga catgctcttt ccctcccgcg cctagtagac gcggcagagg
     1621 acttcgccac cgacattagc gtcgcgtgcg gcggagtccg acatgacgcc cttcggcgtc
     1681 gaaaggatcg agatgcccag gccgttcttg atcggcttca ggtccttgat cgacgaatag
     1741 acgcggcggc ccggcttgga cacgcgtgcg atttcggcga tcacgggctc accgtcaaaa
     1801 tacttgagct cgatctcgaa ttcggggaac gcaccgggct tctcgaccag cgagtagccg
     1861 cggatgtagc cttcgtcggc cagcacgtcc aggacacggg cgcgcagctt ggaggctggc
     1921 gtcgcgacct tggcgcgctt gcgcgtggcg gcgttcttga tgcgagcgat caggtcgctc
     1981 aggggatcgt tcatcgac
//
LOCUS       DAOMEO010000619         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5931_length_1998_cov_2.140504, whole genome shotgun sequence.
ACCESSION   DAOMEO010000619 DAOMEO010000000
VERSION     DAOMEO010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_1998_cov_2.140504"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PLH31_13095"
     CDS             <1..116
                     /locus_tag="PLH31_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydrofolate--homocysteine
                     methyltransferase"
                     /protein_id="HQR90167.1"
                     /translation="DHIRHVAEAVAGVTPRQIPERPKAMRLAGLEPFELVG"
     gene            119..520
                     /locus_tag="PLH31_13100"
     CDS             119..520
                     /locus_tag="PLH31_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HQR90168.1"
                     /translation="MTAWAPGPFDNEEAADFIDDLAEAPDWRTVITLMEHAAGVSGYL
                     EEAEGELAVAAAAVVASCVGTGSVLPDSHADLKATLGKPPEGSANLARKALARVVGTA
                     SELDELWQEGEDHDAWLTQIAGLQSLLGGQS"
     gene            517..>1998
                     /locus_tag="PLH31_13105"
     CDS             517..>1998
                     /locus_tag="PLH31_13105"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQR90169.1"
                     /translation="MRSVFVNIGERTNVTGSAKFKKLIVEGNYGEALSVARQQVEAGA
                     QVIDVNMDEGLLDSRLAMVTFLNLMAAEPDIARVPVMIDSSKWEVIEAGLKCVQGKAI
                     VNSISMKEGEEKFIEQARLCLRYGAAVVVMAFDEVGQADTAARKVEICERAYRILVDQ
                     VGFPPEDIIFDPNIFAVATGIEEHDNYAVDFIEATREIKTRCPYARISGGVSNVSFSF
                     RGNEPVRRAIHSVFLYHAIAAGMDMGIVNAGDLPVYDTLDPELREAVEDVILNRPQRT
                     NVSNTERLVDLAPRYKGDKSQVQTANLEWRKGSVNARITHALVNGITEFINEDTEEAR
                     LSVERPLHVIEGHLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAWLEPFMEAEKA
                     GKPREQAGRILMATVKGDVHDIGKNIVGVVLGCNGYDIVDLGVMVPAQKILDAAREHK
                     ADIIGLSGLITPSLEEMAHVAREMQRQGFTIPLLIGGATTSLAH"
BASE COUNT          364 a          654 c          671 g          309 t
ORIGIN      
        1 cggatcatat ccgtcacgtg gccgaggccg tcgcgggcgt cacgccgcgt caaatccccg
       61 agcggcccaa ggccatgcgc ctggcgggcc tcgaaccttt tgaactggta ggctgagcat
      121 gaccgcctgg gctcccggac ccttcgacaa cgaagaagcg gccgacttca tcgatgatct
      181 ggccgaggcg ccggactggc gcacggtgat caccctgatg gagcacgccg ctggcgtcag
      241 cggctatctg gaagaggccg agggcgaact ggccgtcgcc gctgcggcgg tagtcgcctc
      301 gtgcgtcggg acggggtcgg tgctgcccga cagccacgcc gacctgaaag ccaccctggg
      361 caaaccgccg gagggctcgg ccaatctggc gcgcaaggcc ctggctcgtg tggtcggcac
      421 ggcttccgaa ctcgatgagc tttggcagga gggggaggac cacgacgcat ggctgaccca
      481 gattgccggg ttgcaatccc tgttgggcgg tcagtcgtga ggtccgtctt cgtcaatatc
      541 ggtgagcgca ccaacgtcac cggctcggcc aagttcaaga agctgatcgt cgagggcaac
      601 tacggcgaag ccctgtcggt cgcgcgccag caggtcgagg ccggcgccca ggtcatcgac
      661 gtcaacatgg atgagggcct gctcgactcc aggctggcga tggtcacctt cctgaacctg
      721 atggcggccg agcccgacat cgcccgggtg ccggtgatga tcgacagctc caagtgggag
      781 gtgatcgagg ccggcctgaa gtgcgtgcag ggcaaggcca tcgtcaactc gatctccatg
      841 aaggagggcg aggagaagtt catcgagcag gccaggctgt gcctgcgcta cggcgcggcc
      901 gtggtggtca tggccttcga cgaagtgggc caggccgaca ccgcggcccg caaggtcgag
      961 atctgcgagc gcgcctatcg catcctggtc gaccaggtcg gttttccgcc ggaagacatc
     1021 attttcgacc ccaacatctt cgccgtggca acggggatcg aggagcacga taactacgcc
     1081 gtcgacttca tcgaggcgac gcgcgagatc aagacgcgct gtccctatgc ccggatcagc
     1141 ggcggggtgt cgaacgtctc gttcagcttc cgcggcaacg agcccgtgcg ccgggccatc
     1201 cactcggtgt tcctgtacca cgccatcgcg gcgggcatgg acatgggcat cgtcaacgcc
     1261 ggcgacctgc cggtctatga caccctggac cccgaactgc gcgaggccgt cgaggacgtt
     1321 atcctcaacc gcccccagcg gaccaacgtc tccaacaccg agcggcttgt cgatctggcc
     1381 ccgcgctaca agggcgacaa gagccaggtc cagaccgcca acctggaatg gcgcaagggc
     1441 agcgtgaacg cgcgcatcac ccacgccctg gtcaacggca tcaccgagtt catcaacgag
     1501 gacaccgaag aggcgcgcct gtcggtcgag cggccgctgc acgtcatcga aggccatctg
     1561 atggacggca tgaacgtggt cggcgacctg ttcggctcgg gcaagatgtt cctgccgcag
     1621 gtggtcaagt cggcccgtgt gatgaagcag gccgtggcct ggctggagcc gttcatggag
     1681 gccgagaagg ccggcaagcc gcgcgagcag gctggccgca tcctgatggc cacggtcaag
     1741 ggcgacgtcc acgacatcgg caagaacatc gtcggcgtgg tgctcggctg caacggctac
     1801 gacatcgtcg acctcggggt gatggtgccg gcgcagaaga tcctcgacgc cgcgcgcgag
     1861 cacaaggccg acatcatcgg cctgtccggc ctcatcacgc cctcgctgga ggaaatggcg
     1921 cacgtcgcgc gcgaaatgca gcgccagggc ttcaccattc cgctgctgat cggcggcgcc
     1981 accacctcgc tggcccac
//
LOCUS       DAOMEO010000620         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5946_length_1996_cov_2.393096, whole genome shotgun sequence.
ACCESSION   DAOMEO010000620 DAOMEO010000000
VERSION     DAOMEO010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5946_length_1996_cov_2.393096"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLH31_13110"
     CDS             complement(<1..414)
                     /locus_tag="PLH31_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90170.1"
                     /translation="MAGKIGSSALMGLFANKTGNDNLMTLALRHGRGPIFVAAAFSLV
                     TNLLYLAMPIYTNQIYSRVLSSGSTPTLIVLTVGVIFIFAVSGLIDAIRAQILTAFGV
                     IFDQRVSGHIFSALFDGVVRRQAQAGSQALRDLDQF"
     gene            complement(569..757)
                     /locus_tag="PLH31_13115"
     CDS             complement(569..757)
                     /locus_tag="PLH31_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90171.1"
                     /translation="MFAAYSDQALQEKGRREGPATDLVAQVGFTFDDAMLGEAHNKIS
                     IGHAAPLELGLEDALGVL"
     gene            964..1623
                     /locus_tag="PLH31_13120"
     CDS             964..1623
                     /locus_tag="PLH31_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR90172.1"
                     /translation="MKIVAIVSQKGGAGKTTLAVHLAARAAHDGRQAVIVDLDPQATA
                     SSWGDWRGGDNPLVVAAPHTRLEATLAEAERSGVDLVIIDTPPSADAAAVAAARAADL
                     VLIPTRASAFDLHAIKTTGELMKIAQKPAYVVLNAVPPRSASVIDEAAEVVKSLSLGL
                     APACLVDRAAFRHAVINGQTAVEFEPSSKAASEVHILYTWMCGQLDMPTKKSVKTRSA
                     A"
     gene            1767..1913
                     /locus_tag="PLH31_13125"
     CDS             1767..1913
                     /locus_tag="PLH31_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90173.1"
                     /translation="MIAGYFSPELARAIKILAVERGVTVQALIGEGLDAVLRQHQRHP
                     FGER"
BASE COUNT          428 a          629 c          592 g          347 t
ORIGIN      
        1 aaactggtca agatctcgca gcgcctgcga tccggcctga gcttgccggc ggacaacgcc
       61 atcaaagagg gccgaaaaaa tatggcccga cacccgctga tcgaaaatca cgccaaaggc
      121 agtgaggatt tgcgcacgga tcgcgtcgat cagccctgac acggcgaata taaagattac
      181 cccaaccgtt aggacaatca aagtaggcgt gctgccgcta gaaagaaccc gactatagat
      241 ctggttagtg tagattggca tcgccaaata aagaagattc gtgacaaggc tgaaagccgc
      301 cgcgacaaaa attggtcctc gtccgtggcg caaagcgagc gtcatcaggt tatcattacc
      361 tgttttgttc gcaaaaagac ccataagtgc ggaacttccg atcttaccag ccaatcccca
      421 atgggcagcc cggtcggctg ggggggctcc caatcagcct tttcctggcg gcgagcaatg
      481 aggctggtgc ctattcatgt caatggtgtt agcatcatat tcgatgacaa aagcagcgcc
      541 tttgacgaca cttacgagga aggcccgatc acaggacgcc aagggcatcc tcaaggccga
      601 gctcaagcgg cgcggcatga cctatgctga tcttgttgtg cgcctcgccc agcatggcgt
      661 cgtcgaaagt gaagccaacc tgcgcaacaa gatcagtcgc gggtccttca cggcggcctt
      721 tttcctgcag tgcttgatcg ctgtaggctg cgaacatgtg actatccagg cgccgcgggc
      781 ggacgtcact taagcgcacg cgtggtaaca cctacaatcg gacacgtcga cgtgtccttg
      841 cgaggacgcg tcctcacgag gacagggccg catgaagaca ggtggacgtc agccccaaat
      901 caactcagtc ttgttaatct aaaaattgcc gcgcctcgcg gacgcgtcca catgaggaca
      961 caaatgaaga tcgtcgccat cgtcagtcag aaaggcggcg cgggaaaaac caccctggcc
     1021 gtgcatctgg ccgcccgcgc cgcccatgac ggccgacaag ccgtcatcgt ggacctggac
     1081 cctcaggcca cggcttcctc ttggggcgac tggcgtggcg gcgacaatcc gttagtggtg
     1141 gccgcgccgc acactaggct cgaagcgacg ctggccgagg ccgagaggag cggcgtggat
     1201 ctggtgatca tcgacacccc gccgtccgcc gatgccgcag cagtcgccgc cgcccgcgcg
     1261 gctgacctgg tgctgatccc gacccgcgcc tcggccttcg acctgcatgc gatcaagaca
     1321 accggcgagc tgatgaagat cgcgcaaaag ccggcctatg tcgtgctcaa cgccgtgccc
     1381 ccccgttccg ccagcgtgat cgacgaagcc gccgaagtgg tcaaaagctt gtcactgggt
     1441 cttgccccgg cttgtctggt agatcgcgcc gcctttcgcc acgccgtgat caatggccag
     1501 acggccgtgg aattcgagcc tagcagcaag gccgccagcg aagtccacat cctatacacg
     1561 tggatgtgtg gacagctcga catgcccacc aagaagtcgg tcaagacccg gagcgccgcg
     1621 tgagcgcccg tcccagcctt gccagccgcc tggctgccgc tgcgggggcc cctgccccct
     1681 cccctgccac tgaagcgcca gttcccgaaa cggtagccgc taagggcgtc gcgacggcgc
     1741 cggccaaggc gcgccagggc aagaccatga tcgcgggcta tttctcgccg gagctggccc
     1801 gggccatcaa gattctggcc gtggagcgcg gtgtgaccgt tcaagcgctg attggtgaag
     1861 gtctggacgc ggtgctgcgc cagcatcagc gccatccttt cggcgaacgg taggcggcgg
     1921 gatatggtca agcgcaagga tccgaacaac accgagttcg atttcttcat cccactgatg
     1981 cacgacgtcc cgctga
//
LOCUS       DAOMEO010000621         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5947_length_1996_cov_2.228748, whole genome shotgun sequence.
ACCESSION   DAOMEO010000621 DAOMEO010000000
VERSION     DAOMEO010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5947_length_1996_cov_2.228748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /gene="glmU"
                     /locus_tag="PLH31_13130"
     CDS             <1..1300
                     /gene="glmU"
                     /locus_tag="PLH31_13130"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQR90174.1"
                     /translation="VLHKIAGRALLDHAIDAAEALGCARIIVVVGTHSPAVGVHARKR
                     LGEAAVVIQDPPMGTGHAVLAAKAALADFDGDVVVTFADCPLLTAPVIAPLFDLRASG
                     ADVAVLGFEAHDPGAYGRLILAPGHVLLRIVEAKDADDSVKQVKHCNSGVLAADRALL
                     FDILGQVGNDNANKEYYLTDVVGIAHGRELSTRATFAAEASVQGVNSQAELAAAEAVW
                     QKRRRNALMVEGVTMPAPDTVHLAWDTKLAAGVTVEPFVVFGPGVSVASGAVIKAFSH
                     LEGAAVGEGALIGPYARLRPGAEIGAEAHIGNFVEVKKVKVGAGAKANHLSYLGDGSI
                     GEKANIGAGTIFCNYDGFEKFETHVGKGAFIGSNSALVAPVRVGDGAMTGSGSVITKD
                     VPEGALALSRSDQKIKNGWAEAFRTLKLAKKAKAAKKDRA"
     gene            1297..1977
                     /gene="rpiA"
                     /locus_tag="PLH31_13135"
     CDS             1297..1977
                     /gene="rpiA"
                     /locus_tag="PLH31_13135"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQR90175.1"
                     /translation="MSADDQKRLSGEAAAELVEDGMVVGLGTGSTAAWFVKALAARKL
                     DVRGVPTSDATANLARELGIPLAALDDVKVIDLTVDGADEIGPGLSLIKGGGAALLRE
                     KLVWEASKRCVVIADAAKHVKVLGKFPLPIEVVRFGHVHTGQRLADIAAEFELPPPRL
                     RMADRGVVVTDGGNLIYDMASGRIEEPAGLAAALKAVTGVVDHGLFLDLADEALLGTD
                     TGVVRLVP"
BASE COUNT          316 a          670 c          706 g          304 t
ORIGIN      
        1 ggtgctgcac aagatcgccg gccgggcctt gctggaccac gccatcgacg ccgccgaggc
       61 gctgggctgc gcccggatca tcgtggtggt cggaacccac agccccgccg tgggcgtcca
      121 cgcccgcaag cgtctgggcg aggcggcggt agtcatccag gatccgccga tgggcaccgg
      181 ccacgccgtg ctggccgcca aggcggccct ggccgatttc gacggcgacg tggtcgtcac
      241 cttcgccgac tgcccgctgc tgaccgcccc ggtcatcgcg ccgctgtttg atctgcgcgc
      301 ctcgggcgcc gatgtcgcgg tgctggggtt tgaggcccac gatcccggcg cctatggccg
      361 cctgatcctg gcgcctggcc atgtgcttct gcgcatcgtc gaggccaagg acgctgatga
      421 cagcgtcaag caggtcaagc actgcaattc cggcgtgctg gccgctgatc gggccctgct
      481 gttcgacatc ctgggccagg tcggcaacga caacgccaac aaggaatact acctgacaga
      541 cgtggtcggc atcgcccacg ggcgcgagct gtcgacccgg gcgaccttcg ccgccgaggc
      601 ttcagtgcag ggcgtcaact cgcaggccga actcgccgcc gccgaggccg tctggcaaaa
      661 gcgccgccgc aacgccctga tggtcgaggg cgtcaccatg ccggcgccag acaccgtcca
      721 cctggcctgg gacaccaagc tcgccgccgg ggtcacggtc gagccgttcg tggtcttcgg
      781 gcccggcgtc agcgtcgcga gcggcgcggt gatcaaggct ttcagccatc tggaaggcgc
      841 ggccgtgggc gagggggccc tgatcggtcc ctatgcccgc ctgcgcccgg gcgccgagat
      901 cggcgccgag gcccacatcg gtaacttcgt ggaagtgaag aaggtcaagg tcggggccgg
      961 cgccaaggcc aaccacctca gctatctggg cgacggctcg atcggcgaaa aggccaatat
     1021 cggggccggc acgatcttct gtaattacga cggcttcgag aagttcgaga cccatgtcgg
     1081 caagggcgcc ttcataggct ccaacagcgc gctggtggcg cccgttcggg tgggcgacgg
     1141 ggcgatgacg gggtctggct cggtgatcac caaggatgtg ccggagggcg cccttgcgct
     1201 gtcgcgcagt gatcagaaaa tcaagaacgg ctgggccgag gcgttcagga cgctgaagct
     1261 cgccaagaag gccaaggccg cgaagaagga cagggcatga gcgccgacga tcaaaagcgc
     1321 ctgtcgggcg aggctgccgc cgagctcgtt gaagacggca tggtcgtggg cctgggcact
     1381 ggctcaaccg ccgcctggtt tgtgaaggcc ctggccgcgc gcaagctcga cgtccgcggc
     1441 gtgccgacct ccgacgccac cgccaacctg gcgcgggagc tgggcatccc gctggcggcg
     1501 ctggacgacg tcaaggtgat cgacctcacc gtggacggcg ccgacgagat cggccccggc
     1561 ctgtccctga tcaagggcgg cggcgcggcc ctgctgcgcg agaagctggt ctgggaagcc
     1621 tccaaacgct gcgtggtcat cgccgacgcc gccaagcatg tgaaggtgct gggcaagttc
     1681 ccgctgccca tcgaggtcgt gcgctttggc cacgtccata ccggccaacg cctggccgac
     1741 atcgccgccg agttcgaact gccgccgccg cgtttgcgga tggctgatcg cggcgtggtg
     1801 gtgaccgacg gcggcaacct gatctacgac atggcctcgg gcaggatcga ggagccggcc
     1861 ggtctggccg ccgccctgaa ggccgtgaca ggcgtcgtcg atcatggcct gttcctggac
     1921 cttgccgacg aggcgctgct gggaaccgac acgggcgtgg tgcgcctggt cccttgagtg
     1981 cctagataga gcgccc
//
LOCUS       DAOMEO010000622         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5952_length_1995_cov_1.974227, whole genome shotgun sequence.
ACCESSION   DAOMEO010000622 DAOMEO010000000
VERSION     DAOMEO010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5952_length_1995_cov_1.974227"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>394)
                     /locus_tag="PLH31_13140"
     CDS             complement(<1..>394)
                     /locus_tag="PLH31_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR90176.1"
                     /translation="IGRVSGRQVMIVCNDPTVKGGAYFPMTVKKHLRAQEIAEQNHLP
                     CVYLVDSGGANLPHQAEVFPDREHFGRIFFNQANMSAKGIPQIACVMGSCTAGGAYVP
                     AMSDETVIVRKQGTIFLAGPPLVKAATGE"
     assembly_gap    395..428
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<429..692)
                     /locus_tag="PLH31_13145"
     CDS             complement(<429..692)
                     /locus_tag="PLH31_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HQR90177.1"
                     /translation="MSAPVLMSSLSTESDQFKANARHNRDLVETLRAAVAKASLGGDD
                     RARERHVSRGKLLPRDRVDRLLDPGSPFLEIGQLAANGLYGDDV"
     gene            complement(689..1843)
                     /locus_tag="PLH31_13150"
     CDS             complement(689..1843)
                     /locus_tag="PLH31_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HQR90178.1"
                     /translation="MMTNSAPSMDFALGETADAIRDTTARFAADKIAPIAARIDETNA
                     FPRELWVPMGDLGLHGITVEEEFGGLGLGYLEHVVAMEEISRASASVGLSYGAHSNLC
                     VNQIRRWGTPEQKNRYLPKLISGEHVGSLAMSEAGSGSDVVSMKLRAELKGDRYVLNG
                     TKFWITNAPHADTLVVYAKTSDRGITAFLIEKGMKGFSVSKKLDKMGMRGSDTAELVF
                     EDCEVPEDNIMGPVGGGAGVLMSGLDYERTVLSGGPLGIMQAALDVVLPYVRERKQFG
                     KAIGSFQLMQAKVADMYVALNSARAYVYAVARACDAGRTTRFDAAGAILLASENAVKV
                     SLEAVQALGGAGYTKEWPVERLVRDAKLYDIGAGTNEIRRFLIGRELLGS"
     gene            complement(1921..>1995)
                     /locus_tag="PLH31_13155"
     CDS             complement(1921..>1995)
                     /locus_tag="PLH31_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90179.1"
                     /translation="AICFVVSAYLLLRKLAGPDQDDED"
BASE COUNT          345 a          659 c          619 g          338 t
ORIGIN      
        1 cctcgcctgt cgcggccttc accagcggcg gaccggccag gaagatcgtg ccctgcttgc
       61 gcacgatcac cgtctcgtcc gacatcgcag gcacataggc gccgcccgcc gtgcaggatc
      121 ccatgacgca ggcgatctgg ggaatgccct tggccgacat gttggcctgg ttgaagaaga
      181 tccggccaaa gtgttcgcga tcgggaaaga cctcggcctg gtgcggcagg ttggccccgc
      241 cgctatcgac caaatagacg caaggcagat ggttttgctc ggcgatctcc tgagcgcgca
      301 ggtgcttctt caccgtcatc gggaaatagg ccccgccctt aacggtcggg tcgttgcaga
      361 cgatcatcac ctggcggccc gacacccgac cgatnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnga catcatcgcc atagaggccg ttggcggcca gctggccgat ttccaaaaag
      481 ggtgagccgg gatccagaag ccggtcgacc cggtcccgcg gcagcagctt gccgcgggaa
      541 acatgccgct cccgagcccg gtcgtcgcca cccaggctcg cctttgcgac agcggcgcga
      601 agggtctcga ccagatcgcg gttgtggcgc gcgttggcct tgaactgatc gctctcggtc
      661 gagagcgacg acatcaggac cggcgcgctc atgaccccaa caactcgcga ccgatcagga
      721 agcggcggat ctcgttggtg ccggcgccga tgtcatagag cttggcgtca cggacgagcc
      781 gttcgaccgg ccattccttg gtgtagccgg cgccgcccag ggcctgcacg gcttccaggc
      841 tgaccttcac ggcgttctcc gaggccagca ggatcgcgcc tgccgcgtcg aagcgcgtgg
      901 tcctacccgc gtcacaggcc cgcgccaccg catagacata ggcccgggcc gaattgaggg
      961 cgacgtacat gtcggccacc ttggcctgca tcagctggaa cgagccgatg gccttgccga
     1021 actgcttacg ctcgcggaca tagggcagga ccacgtccag ggccgcctgc atgatgccga
     1081 gcggcccgcc cgacaggact gtgcgttcgt agtcgaggcc gctcatcaac acccccgcgc
     1141 cgccgccgac cgggcccatg atgttgtcct ccgggacctc gcagtcctcg aacaccagct
     1201 cggcggtgtc ggagccgcgc atgcccatct tgtcgagctt cttggaaacg ctgaagccct
     1261 tcatgccctt ctcgatcaga aaggcggtga tgccccgatc gctggtcttg gcgtaaacca
     1321 ccagggtgtc ggcatgcggc gcgttggtga tccagaactt cgtgccgttc agcacgtagc
     1381 ggtcgccctt gagctcggcc cgcagcttca tcgagaccac gtccgaaccc gatcccgcct
     1441 cgctcatggc cagagagcca acatgctcgc cgctgatcag cttgggcagg tagcggttct
     1501 tctgctcggg cgtgccccag cggcgaatct ggttcacgca gagattggag tgcgcgccgt
     1561 aggacaggcc gaccgaggcc gaagcccggg aaatctcctc catggccacg acgtgttcaa
     1621 gatagcccag gcccagaccg ccgaactctt cctcgacggt gatgccgtgc aaaccgaggt
     1681 cacccatcgg gacccaaagc tcgcgcggga aggcgttggt ttcgtcgatc cgggccgcga
     1741 tgggcgcgat cttgtcggcc gcgaagcggg cggtggtgtc tcggatcgcg tcggccgttt
     1801 cgcccagcgc gaagtccatc gagggtgcgg aatttgtcat catggccacc tgatagcaca
     1861 aacgcccgcg gatcaacgag acccgcgggc gttcatgctt tccagccttt cagccggaat
     1921 ttagtcttcg tcatcctggt cgggaccggc cagcttgcgc agcagcaggt aggccgaaac
     1981 cacgaaacag atcgc
//
LOCUS       DAOMEO010000623         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5960_length_1993_cov_3.390093, whole genome shotgun sequence.
ACCESSION   DAOMEO010000623 DAOMEO010000000
VERSION     DAOMEO010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_1993_cov_3.390093"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1993
                     /locus_tag="PLH31_13160"
     CDS             <1..>1993
                     /locus_tag="PLH31_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR90180.1"
                     /translation="SAVATRDAPTVESAITNLRQRLGLDLAFTVGVEGDVIGLDPALL
                     DLDKLWAALDAEDGAQGVFVIDNAPYQIISAPIMAPTLNGWIVFAAKLDDREMQALEG
                     LSAIDLDAQVFDQRNGQWITTDTSFDPERIGAYVDQALQARTHEPELLKAGRADNLVL
                     VKPLKSLSQDGAAALVLRYPMAKAMAPYRPLFAAVILAGLLGAALVVVASWALSRGLA
                     RPIAALDAAARRLQRGEDVRVEVQTHDEIGRLASSFNLMAAEIRDREAKMIHQTLHDA
                     ETGLPNRRALEREISARLAGAAEGEQVIISAVGLDRFPFMRGAIGYTLATQLIADLGE
                     RITAISPGLLVGRLTTSTLCVVSNLTDLAALLPLIDRSVQDLERPIRVGGERVDIHLT
                     HGLAATTAGALDAPRLIEQALIALDQGRAANRKVMLFDPVAYGDPASNLSLMSEMLDA
                     VTTGALFLNHQPKLDLRTGQITGVEALVRWKHPTRGLMRPDLFVGMAEETGHIRALTD
                     YVLTQAIADQKILRAAGHDVLMSVNISGRLIDDADFADRAIEQVTREGARLCFEITET
                     AVIGNPEVALAVLERFAAAGIPVSIDDYGSGLSSLAYLKQIRADELKIDKAFVMSLEA
                     GGKDALLVKSTIDLAHSLGMKVTAEGVETREALAALQLMG"
BASE COUNT          327 a          718 c          631 g          317 t
ORIGIN      
        1 tctgcggtcg ccacccgcga tgcgcccacc gtcgagtccg ccatcaccaa tctgcgccag
       61 cgtctgggcc tggacctggc cttcacggtc ggcgtcgagg gcgacgtcat cggcctcgat
      121 ccggcccttc tggacctcga caagctatgg gcggcgctgg acgccgaaga cggcgcccag
      181 ggcgtgttcg tcatcgacaa cgcgccctat cagatcattt ccgcaccgat catggcgccg
      241 accctgaacg gctggatcgt cttcgccgcc aagctggacg atcgcgagat gcaggccctg
      301 gagggcctgt cggccatcga cctggacgcc caggtgttcg accagcgcaa tggccagtgg
      361 atcaccaccg acaccagctt tgatcccgaa aggatcggcg cctatgtcga ccaggccttg
      421 caggccagga cccacgagcc cgaactgctg aaggccggcc gcgctgataa tctggtgctg
      481 gtcaaacccc tcaaatcgct gagccaggac ggcgcggcgg cgctggtcct gcgctatccg
      541 atggccaagg ccatggcgcc ctatcgcccg ctgttcgcgg ccgtcatcct ggccggactg
      601 cttggcgcgg ctctggtggt tgtcgccagc tgggccctgt cgcgcgggct ggcccgtccc
      661 atcgccgccc tggacgccgc agcccgtcgt ctgcagcgcg gcgaggacgt tcgggttgag
      721 gtccagaccc atgacgaaat cggacgtctg gccagcagct tcaacctgat ggccgcggag
      781 atccgtgacc gcgaagccaa gatgatccat caaacgctgc acgacgcgga aaccggcctg
      841 cccaaccgcc gggccctgga gcgcgagatc tccgcccgcc tggccggcgc cgcagagggc
      901 gaacaggtga ttatcagcgc cgtcggcctg gatcgctttc ccttcatgcg cggcgccatc
      961 ggctacaccc tggccaccca gctgatcgcg gatctgggcg agcggatcac ggcgatcagc
     1021 cccggcctgc tggtggggcg cctgaccaca tcgaccctgt gcgtcgtctc caacctcacc
     1081 gatcttgccg ccctgctgcc gctgatcgat cgctcggtgc aagacctcga acgccccatc
     1141 cgggtgggcg gcgagcgggt cgacattcac ctgacccacg gcctggcggc caccacagcc
     1201 ggcgcgctgg acgccccgcg cctgatcgag caggccctga tcgccctgga ccagggccgc
     1261 gcggccaacc gcaaggtgat gctgttcgac cccgtggcct atggcgaccc cgccagcaat
     1321 ctgtcgctga tgagcgagat gctggatgcc gtgaccaccg gcgcgctctt cctgaaccat
     1381 caacccaagc tggacctgcg caccggccag atcaccggcg tcgaggccct cgtgcgctgg
     1441 aagcacccca cgcgcggcct gatgcgcccg gacctgttcg tcggcatggc cgaggagacg
     1501 ggccacattc gcgccctgac cgattacgtg ctgacccagg ccatcgccga ccagaagatc
     1561 ctgcgcgcgg ccggtcatga cgtgctgatg tcggtcaaca tctccggccg cctcatcgac
     1621 gacgccgact tcgccgaccg ggccatcgag caggtgacac gtgaaggcgc gcggctgtgt
     1681 ttcgaaatca ccgaaacggc cgtgatcggc aatcccgagg tggctctggc cgttctggaa
     1741 cgcttcgccg ccgccggcat ccccgtctcg atcgacgact atggctcggg cctgtcgtca
     1801 ctggcctatc tgaagcagat ccgcgccgac gaactgaaga tcgacaaggc cttcgtgatg
     1861 tcgctggaag ccggcggcaa ggatgcgctg ctggtcaagt ccaccatcga cctcgcccac
     1921 agcctgggca tgaaggtcac cgccgagggc gtcgagaccc gtgaggccct ggccgcgctg
     1981 caactgatgg gct
//
LOCUS       DAOMEO010000624         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_5981_length_1988_cov_2.610450, whole genome shotgun sequence.
ACCESSION   DAOMEO010000624 DAOMEO010000000
VERSION     DAOMEO010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_1988_cov_2.610450"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLH31_13165"
     CDS             complement(<1..534)
                     /locus_tag="PLH31_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HQR90181.1"
                     /translation="MSFSDGEVERYARHLVLREVGGPGQQKLKAARVLLVGAGGLGAP
                     AALYLAAAGVGTLGLADPDAVSLSNLQRQVLYQTADVGRSKVEVASERLTAINPHVTV
                     ATHPVWLDAANARDLVARYDLVLDGTDDFATRFAVSDACLAEGKPLVSGALGRWTGQV
                     GVFHGQPCYRCLVPEVPP"
     gene            575..1138
                     /gene="hslV"
                     /locus_tag="PLH31_13170"
     CDS             575..1138
                     /gene="hslV"
                     /locus_tag="PLH31_13170"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQR90182.1"
                     /translation="MQNSNSFPDWHGTTILAVRKNGQTVIAGDGQVSMGPTVVKGNAR
                     KVRRLAGGKVVAGFAGATADAFTLIERLEAKLEQYPDQLARACVDLAKDWRTDRYLRR
                     LEAMLLVADATAIYTVTGVGDVLEPGESAGGGSVAAIGSGGNYALAAGKALIDLDLSA
                     EEIARKAMAIAAEICVYTNGNLTVESL"
     gene            1138..>1988
                     /gene="hslU"
                     /locus_tag="PLH31_13175"
     CDS             1138..>1988
                     /gene="hslU"
                     /locus_tag="PLH31_13175"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQR90183.1"
                     /translation="MTEFSPREIVSELDRFIVGHTDAKKAVAVALRNRWRRRRVPADL
                     RDEVTPKNILLIGPTGVGKTEIARRLAKLAQAPFLKVEATKFTEVGYVGRDVDQIVRD
                     LVESALGMVRDKRRAGVRARAEAAAEERILDALTGPGSTAARESFRKKLRAGELDDKE
                     VELQLADTGGGGMFEIPGQPGGSVLNLSEMMKSFGGGRTKTHKTTVAGAHAPLIAEES
                     DKLLDQEALTQEALELAENHGIVFLDEIDKVASSSQRGGADVSREGVQRDLLPLIEGT
                     TVSTKYGP"
BASE COUNT          344 a          697 c          675 g          272 t
ORIGIN      
        1 tggcggaacc tcgggcacca ggcagcgata gcagggctgg ccgtggaaca ctccgacctg
       61 acccgtccag cggcccaggg cgccgctgac caggggcttg ccctcggcca ggcaggcgtc
      121 gctgacggca aagcgggtgg caaagtcgtc tgtgccgtcc agcaccaggt cataccgcgc
      181 gacgaggtcg cgagcattgg cggcgtccag ccataccggg tgggttgcga cggtgacgtg
      241 cgggttgatc gcggtcaggc gctcggaagc gacctcgacc ttggaccggc cgacatcggc
      301 ggtctggtac agcacctgcc gctgcaggtt cgacagcgac acggcgtcag ggtcggccag
      361 gcccagcgtc cccaccccgg cggcggccag atagagcgcg gccggcgcgc ccagaccacc
      421 cgcgccgacc agcagcacac gggctgcctt cagcttctgc tgccccggtc cgcccacctc
      481 gcgcagcacc agatggcggg catagcgttc gacctcgccg tccgaaaagc tcatcgcgct
      541 tgatctccgc agccagcctc gccacatgcg agacatgcaa aacagcaatt ccttcccaga
      601 ctggcacggc acgaccattc tggcggtgcg caagaacgga cagaccgtga tcgccggaga
      661 cggccaggtc tccatgggcc caaccgtcgt caagggcaac gcccgcaagg tgcgcaggct
      721 ggccggcggc aaggtggtgg ccggtttcgc cggcgccacg gccgacgcct tcaccctgat
      781 cgagcggctt gaggccaagc tggaacagta tcccgaccag ctggcccgcg cctgcgtgga
      841 tctggccaag gactggcgca ccgaccgcta tctgcgccgc ctggaggcca tgctgctggt
      901 ggccgacgcc accgccatct acaccgtcac cggcgtgggc gacgtgctgg agcccggcga
      961 aagcgccggc gggggttcgg tggcggccat cggctcgggc ggcaactatg ccctggcggc
     1021 cggcaaggcc ctgatcgacc tggatctttc ggccgaagag atcgcccgca aggcgatggc
     1081 gatcgccgcc gagatctgcg tctacaccaa cggcaatctg acggttgaaa gcctgtaatg
     1141 accgagttct ctccccgcga aatcgtttcc gagctcgacc ggttcatcgt cggccatacc
     1201 gacgccaaga aggccgtcgc cgtcgccctg cgcaaccgct ggcgccgccg tcgcgtgccg
     1261 gccgacctac gcgatgaggt cacccccaag aacatcctgc tgatcggccc caccggcgtc
     1321 ggcaagaccg agatcgcccg ccgcctggcc aagctggccc aggccccgtt cctgaaggtg
     1381 gaggccacca agttcaccga ggtgggctat gtgggccgcg acgttgacca gatcgtccgc
     1441 gacctggtgg aaagcgccct gggcatggtg cgcgacaagc gccgggccgg cgtgcgggcc
     1501 cgcgccgagg cggccgctga ggagcgcatc ctcgacgccc tgaccggacc cggctcgacc
     1561 gccgcccgcg aaagcttccg caagaagctg cgggccggcg agctggacga caaggaagtg
     1621 gagctgcagc tggccgatac cggcggcggc gggatgttcg agatccccgg tcagcccggc
     1681 ggctcggtgc taaacctttc cgagatgatg aaatcgttcg gcggcggtcg caccaagacc
     1741 cacaagacca cggtggccgg cgcccacgcc ccgctgatcg ccgaggaaag cgacaagctg
     1801 ctggatcagg aggccctgac ccaggaagct ctggaactgg ccgagaacca cggcatcgtc
     1861 ttcctggacg agatcgacaa ggtggcctcg tcctcccagc gtggcggggc cgatgtgtcg
     1921 cgcgagggcg tgcagcgcga cctgctgccg ctgatcgagg gcacgacggt ttcgaccaag
     1981 tatggccc
//
LOCUS       DAOMEO010000625         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6002_length_1982_cov_2.375714, whole genome shotgun sequence.
ACCESSION   DAOMEO010000625 DAOMEO010000000
VERSION     DAOMEO010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6002_length_1982_cov_2.375714"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLH31_13180"
     CDS             complement(<1..602)
                     /locus_tag="PLH31_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR90184.1"
                     /translation="MMKKTALVALLMGAAALTACTTTDPYTGMPIRNNTGTGALSGAG
                     VGAVLGYLTNTNSGEQGRKNALIGAGIGALAGGAVGNYMDRQQADFRRSLEGSGVMIR
                     RNGDQIVLVMPSDVTFAVDQSDVQPQFTRVLDDVARTLNAYPQTTIDVVGHADSSGAD
                     AYNQSLSERRASSVAGYLTGPGRVLPDRIFVAGMGERQPIA"
     gene            799..1734
                     /locus_tag="PLH31_13185"
     CDS             799..1734
                     /locus_tag="PLH31_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HQR90185.1"
                     /translation="MSTSAIEPVSFTLYQKDFTGFAQALGASFERYGFAVLSEYDLDQ
                     DRIDAAISDTKAFFALPVEIKKQYAGVKGGARGYIPFGVETAKGATHYDLKEFWHMGR
                     DLPPGHRFRATMADNVWPAEIPAFKHDVSWLYNALDGMGGKVLEAIATYLKLDRNFFA
                     PTIENGNSVLRLLHYPPIPMDATGVRAGAHGDINTITLLLGAEEGGLEVLDRDGQWLP
                     INPPPGCLVINIGDMLERLTNHVLPSTQHRVVNPPPERRGVPRYSTPFFLHFASDYEI
                     KTLPGCVTPDNPDRYPESITADEFLQQRLREIKLA"
     gene            complement(1773..>1982)
                     /locus_tag="PLH31_13190"
     CDS             complement(1773..>1982)
                     /locus_tag="PLH31_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG"
                     /protein_id="HQR90186.1"
                     /translation="SRQKKTCAAAKAKGVIIYSIGVGVSAHSKGILQECASTSDKYYD
                     VTDSAQLAAVFDAIAGSIQNLRIAK"
BASE COUNT          326 a          643 c          642 g          371 t
ORIGIN      
        1 gcgatcggct gacgttcgcc catgccggcc acgaagatcc ggtcgggcag aacgcggccg
       61 gggccggtca gatagccggc gaccgagctg gcgcgacgct cggacagcga ctggttatag
      121 gcgtcggcgc cgctgctgtc ggcatgacca accacgtcga tcgtggtctg gggataggcg
      181 ttcagggtgc gggccacgtc gtccagcacg cgggtgaact gcggctgcac gtcggactgg
      241 tcaaccgcga aggtcacgtc gctgggcatg accagaacga tctggtcgcc gttgcggcgg
      301 atcatcacgc ccgagccttc caggctgcga cggaagtcgg cctgctggcg gtccatgtag
      361 ttgccgaccg cgccaccggc cagggcgccg atgcccgcgc cgatcagggc gttcttgcgg
      421 ccctgctcgc ccgaattggt gttggtcaga tagcccagca ccgcgccgac gccggcgccg
      481 ctcagcgcgc cggtgccggt gttgttgcgg atcggcatgc cggtataggg atcggtggtc
      541 gtacaggcgg tgagagcggc ggcgcccatc agcagggcga cgagtgcagt cttcttcatc
      601 atcatggtcg ttgtcttccg tgtccgttcc ggtgcgtgca gccacgcttg atcatcgaga
      661 gggctaacgc ccggctcatg gtcaagttcc atcacgggac atgacacgat gatgaacggg
      721 tcttcgctca cgcctgttcc cggtgtagta agttgcaact tactagtttc ccgactgact
      781 tttcgaaggc tcacccctgt gtccacgtct gcgatcgaac cggtttcttt cacgctctac
      841 caaaaagatt tcaccggctt cgcccaggcg ctgggggcct cgttcgagcg ttacggcttt
      901 gcggtgctgt cggaatacga cctggatcag gaccgcatcg acgcggccat ttccgacacc
      961 aaggccttct tcgccctgcc ggtcgagatc aagaagcaat atgccggggt caagggcggg
     1021 gcgcgcggct atatcccgtt cggggtcgag accgccaagg gcgccaccca ttacgacctg
     1081 aaggaattct ggcacatggg ccgcgacctg ccgcccggcc accgcttccg cgccacgatg
     1141 gccgacaatg tctggccggc cgagatcccg gccttcaagc atgacgtgtc gtggctttac
     1201 aatgcgctcg acggcatggg cggcaaggta ctggaggcga tcgccaccta tctgaagctg
     1261 gaccgcaact tcttcgcgcc gaccatcgag aacggcaaca gcgtgctgcg cctgctgcac
     1321 tatccgccga tcccgatgga cgccacgggc gtgcgggccg gggcgcatgg cgacatcaac
     1381 accatcaccc tgctgctggg cgccgaagag ggcgggctgg aggttctgga tcgcgacggc
     1441 cagtggctgc cgatcaatcc gccccccggc tgtctggtga tcaatatcgg cgacatgctg
     1501 gagcggctga ccaaccacgt gctgccctcg acccagcacc gggtggtcaa tccgcccccg
     1561 gaacgtcgcg gcgtgccgcg ctattcgacg ccgttcttcc tgcatttcgc gtcggactac
     1621 gagatcaaga ccctgccggg ctgcgtgacg ccggacaatc cggaccgcta tccggagtcg
     1681 atcaccgccg acgaattcct ccagcagcgc ctgcgcgaga tcaagctggc ctgatttgac
     1741 ccctctccct ctgggagagg gctgatcctc tcttatttgg cgatgcgcag gttctggatc
     1801 gagccggcga tggcgtcgaa caccgccgcc agctgggcgg agtcggtgac atcgtaatac
     1861 ttgtccgagg tcgaggcgca ttcctgcagg atccccttgg agtgggccga taccccgacg
     1921 ccgatgctgt agatgatcac gcccttggcc ttggcggcgg cgcaggtctt cttctggcgg
     1981 ct
//
LOCUS       DAOMEO010000626         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6013_length_1978_cov_2.329693, whole genome shotgun sequence.
ACCESSION   DAOMEO010000626 DAOMEO010000000
VERSION     DAOMEO010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6013_length_1978_cov_2.329693"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLH31_13195"
     CDS             <1..926
                     /locus_tag="PLH31_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HQR90187.1"
                     /translation="EMEEVVKVELAERMSQAPAAPRGLSRSLALDSIAVAQPILRGAS
                     LSEEDLLEVARTRGQDHLRAISQRASVPSAVSDAIVQRGDDDTLGVLLRNEGAVLSRE
                     AHEAVVDRAAANPALHEAVVDRHSLPVDLLNEMYFMVEARLRERIMEKNADVDPATLE
                     AALNAGRKRVAEQDGALPADLAQAEHEFSGIKARGGATPQALAGMLRSKKTTLFLVAL
                     ADMADIDFHTARKILQRRELDALAVVCKAADFDRALFLTFALLILDPSDDVMGKAKAY
                     GELYVALPRESALRTIRFWRLRRQTGDVAAA"
     gene            1021..>1978
                     /locus_tag="PLH31_13200"
     CDS             1021..>1978
                     /locus_tag="PLH31_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR90188.1"
                     /translation="MIRASRLTLLAAAGLSLAACSALPSLPLPSMPSMGQREAIAPAQ
                     PAGPTLASLKPGQWAHQLSDVAPDPRVRFGVLPSGMRYALMKNATPPQQAALRLWFDT
                     GSMMEADDQQGLAHFLEHMAFNGSKNVPEGEMVKILERHGLAFGADTNASTGLDETIY
                     QLDLPKTDDATVDTSLMLLRETASELTIAPNAVDRERGVVLSEERTRDSPGYRVAIKS
                     MDFQMEGQLPPKRIPIGKTEVLKTAPAQRLRDYYEAYYRPERAVLVAVGDFDLDAMEA
                     KIKARFGDWTATGPAGPDPILGPVAPRTPEAKLVIEAGAPLSL"
BASE COUNT          322 a          735 c          629 g          292 t
ORIGIN      
        1 gcgagatgga agaggtcgtc aaggtcgagc tggccgagcg catgagccag gcgcccgccg
       61 cgccgcgcgg tctcagccgc tccctggccc tggacagcat cgccgtggcc cagccgatcc
      121 tgcgcggagc cagtctgtcg gaagaggacc tgctggaagt ggcccgcacc cgcggccagg
      181 accatctgcg cgccatctcg cagcgcgcca gcgtgcccag cgccgtatcg gacgccatcg
      241 tccagcgcgg cgatgacgac acgctgggcg tcctgctgcg caacgagggc gcggtgctgt
      301 cgcgcgaagc gcacgaggcg gtcgtcgacc gcgccgccgc caacccggcc ctgcacgagg
      361 cggttgtcga ccgtcacagc ctgccggtcg atctgctgaa cgagatgtac ttcatggtcg
      421 aagccaggct tcgcgagcgg atcatggaga agaacgccga cgtcgatccc gccacgctgg
      481 aagccgccct gaacgccggc cgcaagcgtg tggccgaaca ggacggcgcc ctgcccgccg
      541 acctcgccca ggccgaacac gagttcagcg gcatcaaggc ccggggcggc gcaacgcccc
      601 aggccctggc cgggatgctg cggtccaaga agaccacgct gtttctggtc gccctggccg
      661 acatggccga catcgacttc catacggccc gcaagatcct gcagcgccgt gagctcgacg
      721 ccctggccgt ggtctgcaag gcggccgatt tcgaccgcgc cctgttcctg accttcgccc
      781 tgctgatcct ggatccgtct gacgacgtga tgggcaaggc caaggcctat ggcgagctct
      841 acgtcgccct gcccagggag tcggccctgc ggaccatccg cttctggcgc ctgcgccgcc
      901 agaccggcga cgtcgcagcc gcctgatcgc ggctttcgcg aggccttaga cgccttgctt
      961 gcgccttcac ccccggccaa ggtgcggctt ccaaagagct cacctaaccc tgggtcgtat
     1021 atgattcgcg cttcgcgcct gacgctactg gccgccgctg gcctgtccct cgccgcctgc
     1081 tcggccctgc ccagcctgcc cctgccgtcg atgccctcca tgggccagcg agaggccata
     1141 gcccccgccc agcctgcggg cccgacgctg gcgtcgctga agcccggtca atgggcccac
     1201 cagctttcgg acgttgcgcc cgacccgcgc gtgcgcttcg gcgtgctgcc cagcggtatg
     1261 cgctacgccc tgatgaagaa cgccacgccg ccccagcagg ccgccctgcg cctgtggttc
     1321 gacaccggct cgatgatgga agccgacgac cagcagggcc tggcccactt ccttgagcac
     1381 atggccttca acggcagcaa gaacgtgccc gaaggcgaga tggtcaaaat cctggaacgc
     1441 cacggcctgg cctttggcgc cgacaccaac gcctccaccg gcctggacga aaccatctat
     1501 cagctggacc tgcccaagac ggacgacgcg acggtcgaca cctcgctgat gctgctacgc
     1561 gaaacggcca gcgagctgac gatcgcgccg aacgccgtcg atcgcgagcg cggcgtggtg
     1621 ctgtcggagg aacgcacccg ggacagccct ggctatcgcg tggccatcaa gtcgatggac
     1681 ttccagatgg aaggccagtt gccgcccaag cgcattccta tcggcaagac cgaggtgctg
     1741 aaaaccgcgc cagcccagcg cctgcgcgac tattacgaag cctattatcg tcccgaacgg
     1801 gccgtgcttg tggcggtcgg cgacttcgat ctcgacgcca tggaggccaa gatcaaggcc
     1861 cgcttcggcg actggacagc caccgggcct gccggccccg accctattct cggccccgtc
     1921 gctccccgca ccccggaggc caagctggtc atcgaggcgg gcgcgccgct gtccctgc
//
LOCUS       DAOMEO010000627         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6022_length_1975_cov_2.490625, whole genome shotgun sequence.
ACCESSION   DAOMEO010000627 DAOMEO010000000
VERSION     DAOMEO010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_1975_cov_2.490625"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>301)
                     /locus_tag="PLH31_13205"
     CDS             complement(<1..>301)
                     /locus_tag="PLH31_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR90189.1"
                     /translation="APLTYAAALQAAGQTSPTLQARGLQVESARAAARASGALPDPKL
                     TAGIDNFPISGPPAGRFNGDSMTMARVGVMQDMPSGAKRSAARALAQTDIDVAGAV"
     assembly_gap    302..311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(407..772)
                     /locus_tag="PLH31_13210"
     CDS             complement(407..772)
                     /locus_tag="PLH31_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90190.1"
                     /translation="MRRLLVLFALVGLLYSPAAAVASQQTCVESGMPMAGMATAASEN
                     GKAVAGAPVPCCDHKSKAHDNKACAQACAVMGGVVGALPTAVLFVPPQIEGQPVAEVS
                     LPLKPHPPPRTERPPRSIV"
     gene            complement(818..1750)
                     /gene="copD"
                     /locus_tag="PLH31_13215"
     CDS             complement(818..1750)
                     /gene="copD"
                     /locus_tag="PLH31_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HQR90191.1"
                     /translation="MLEPAVIILRLAQYTGAMILFGSSLFALYALAPSGMAASASLRW
                     LRPLLAWSAAGLLAASLLGLLAQTSVLAGSLSEGLKLSSLSAVVTTMGFGRSALVRAV
                     GAGLALFLLLPRRLDRTSLRVCAMLGAVTCASFAWMGHGAATEGPGGSLHLGADILHS
                     LAAGVWIGALVGFLFLLKRRSSDDAALDHVRHRALHGFSGIGSVLVATLVASGLVNSW
                     FLIGPTRIPGLWTTPYGQLLSLKLLLFAGMLVLAAANRFHLTPTLRVALDTAAAREPA
                     LGALRRSLVIESALAIAVLGLVAGFGMLAPVSAQ"
     gene            complement(1754..>1975)
                     /locus_tag="PLH31_13220"
     CDS             complement(1754..>1975)
                     /locus_tag="PLH31_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HQR90192.1"
                     /translation="FNEKLTPAFSKFELVMPTMNNMAVAVKTSVSKDGLTITGTPQAP
                     LMPGVYKINWHAASSDGHKMDGAVDFTVK"
BASE COUNT          365 a          649 c          676 g          275 t
ORIGIN      
        1 actgcccctg cgacgtcgat atcggtctgc gcaagcgcgc gggccgccga tcgcttcgcg
       61 ccgctgggca tgtcctgcat gacgccgacc ctggccatgg tcatgctgtc gccgttgaac
      121 cggccagccg gcggtcccga gatcgggaag ttgtcgatgc cggcggtcag tttcgggtcg
      181 ggcagagccc cggaggcccg cgccgcggcg cgggcgctct ccacctgcaa gccgcgggcc
      241 tgcaaggtgg gcgaggtctg gccggccgcc tgtagggcgg cggcgtaggt cagtggcgcg
      301 gnnnnnnnnn ncaaaacttt cccattaagc ttcccagctg actatcacgg taaagatgtt
      361 gctggcgaca gcgtgcggag ccacgccgcg cgaagcgcgg ctggcgtcag acgatggatc
      421 tgggcggtcg ttcggtccgc ggcggcggat gcggcttcag aggcagggac acttcggcca
      481 ccggctgtcc ctcgatctgc ggcggcacga agagcacggc cgtcggcagg gcgccgacga
      541 ccccgcccat cacggcgcac gcctgggcgc aggccttgtt gtcgtgcgcc ttggacttgt
      601 ggtcgcagca gggaaccgga gcgcccgcga cggccttgcc gttctcggac gctgcagtgg
      661 ccatgcccgc catcggcatg ccgctctcga cgcaggtctg ctgcgacgcc acggccgcgg
      721 ccgggctgta gagcaggccc acgagggcga aaaggacgag caagcgccgc atgatcgcca
      781 agctatgcct gaatgcaggc caagtcgacg gagccgctca ttgcgccgac accggcgcca
      841 gcatgccgaa cccggcgacc agaccgagca ccgcaatggc gagcgcgctt tcgatgacta
      901 ggctgcggcg cagagcgccc agcgcgggct cccgcgcagc ggcggtatcg agcgccactc
      961 tgagcgttgg cgtcaggtga aagcggttgg cggcggccag caccagcatg ccagcgaaca
     1021 gcaggagctt gagcgatagg agttggccgt agggcgtggt ccagagccca gggatgcgcg
     1081 tggggccgat gaggaaccag ctgttgacca gaccagacgc caccagggtc gccaccagca
     1141 ccgacccgat gccggaaaat ccgtgcagcg ctctgtggcg gacatgatcg agcgcagcat
     1201 catcgctgga tcgtcgcttc agtaggaaca ggaaaccaac gagggcaccg atccataccc
     1261 cagcagccag agaatgtagg atatcggcgc cgagatgcag agagccaccg ggcccttcgg
     1321 tcgccgcccc atgacccatc caggcaaaac tcgcgcaggt caccgcgccg agcatggcgc
     1381 agacccttaa gctcgtgcgg tcgagccggc gagggagcag caagaagagc gccaggccag
     1441 cccctacggc gcggaccagc gccgagcggc cgaagcccat agtggtgacc accgcagaca
     1501 gcgaggagag cttcaggcct tcactgagcg atcccgccaa caccgaggtt tgggcgagca
     1561 aacccagaag gctcgcggcc aacaggcccg cggcgctcca ggccagcagc ggacgcaacc
     1621 agcgcaacga cgccgacgcc gccatgcccg agggggccag ggcgtagagg gcaaagagcg
     1681 acgatccgaa caggatcatc gcccccgtat attgcgccag cctgaggata atgaccgcag
     1741 gctcgagcac ggcctatttg accgtgaagt ccacggcgcc gtccatcttg tgcccgtcgc
     1801 tggaggcggc gtgccagttg atcttataga caccgggcat cagcggcgcc tgcggcgttc
     1861 cggtgatggt gagaccatcc ttggatacgc tggtcttcac cgccacggcc atgttgttca
     1921 tcgtcggcat gaccagctcg aacttcgaga aggccggggt cagcttttca ttgaa
//
LOCUS       DAOMEO010000628         1974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6029_length_1974_cov_2.509119, whole genome shotgun sequence.
ACCESSION   DAOMEO010000628 DAOMEO010000000
VERSION     DAOMEO010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6029_length_1974_cov_2.509119"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..255
                     /locus_tag="PLH31_13225"
                     /pseudo
     CDS             108..255
                     /locus_tag="PLH31_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <266..1116
                     /locus_tag="PLH31_13230"
     CDS             <266..1116
                     /locus_tag="PLH31_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQR90193.1"
                     /translation="ATHGLQITELSTHLQGQLVAVHPAYDELFDGFAPPEVRGKPAER
                     QAWAVGQVKAAAVASRRLGLNAHATFSGALAWPYFYPWPQRPAGLVEEAFAELGRRWK
                     PILDVFETEGVDVCYEIHPGEDLHDGATFERFLDEVGGHPRANILYDPSHFVLQQLDY
                     LGYIDRYHERIKAFHVKDAEFRPSARSGVYGGYQGWAERPGRFRSLGDGQVDFKAIFS
                     KLAQYDYAGWAVLEWECALKHPEQGAREGAPFIRDHIIQVTDRAFDDFAGSAPDAGRN
                     RRLLGL"
     gene            1221..1502
                     /locus_tag="PLH31_13235"
     CDS             1221..1502
                     /locus_tag="PLH31_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR90194.1"
                     /translation="MFEQRCAVCHSLKPAPGKMGPTLAGVVGRKAGTLGGYAYSNALK
                     ASGITWNAQTLDAYVKAPAKTVPGTKMLLGAPDAQQRAAVIQYLASVKK"
     gene            complement(1693..>1974)
                     /locus_tag="PLH31_13240"
     CDS             complement(1693..>1974)
                     /locus_tag="PLH31_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQR90195.1"
                     /translation="TPPANAPTIPDGTWTKVELEVRPNGEVFQKIDGVVVHHYAGLTL
                     DPDDTVAGGAKPYILARGAQPVTEGYIALQSEGHPIEFKDIEIQELTAQ"
BASE COUNT          306 a          660 c          667 g          331 t
ORIGIN      
        1 ggctgggtgg cgctggccac cagcccggct cagccgggtt gggcgaccca agaaggagag
       61 ggccgatgaa gacgctcaag ggaccggcca ttttcctggc ccagttcatc ggcagcgagc
      121 ccccgttcga cacgctggag accatggccg cctgggtcgc cgacctcggc tatgtcgggg
      181 tgcagatgcc caccggcggg gcggactcgt tcttcgacct ggccctggcc gccgagagcc
      241 agacctattg cgacgnnnnn nnnnntggcc acccacggtc tgcagatcac cgagctttcg
      301 acccatctgc agggccaact ggtggccgtg catccggcct atgacgagct gttcgacggc
      361 ttcgccccgc ccgaggtgcg tggcaagccg gccgagcgtc aggcctgggc ggtggggcag
      421 gtcaaggccg ccgccgtcgc cagccggcgc ctgggcctga acgcccacgc caccttttcc
      481 ggcgctttgg cgtggccgta tttctatccg tggccgcagc ggccggccgg tctggtcgag
      541 gaggctttcg ccgagctggg ccgtcgctgg aagccgatcc tcgacgtctt cgagaccgag
      601 ggcgtcgacg tctgctacga gatccatccg ggcgaggatc tgcacgacgg cgcaaccttc
      661 gagcggttcc tggacgaggt gggcggccat ccgcgcgcca atatcctgta tgatcccagc
      721 cacttcgtgc tgcagcagct ggactatctg ggctatatcg accgctatca cgagcggatc
      781 aaagccttcc acgtcaagga cgccgagttc cggccctcgg cccggtcggg cgtctatggc
      841 ggctatcagg gctgggccga gcggccgggg cgcttccgct cgctgggcga tggccaggtg
      901 gacttcaagg cgatcttttc caagctggcc cagtacgact atgccggctg ggcggtgctg
      961 gaatgggagt gcgcgctgaa gcatcccgag cagggcgccc gcgaaggcgc gccgttcatc
     1021 cgcgatcaca tcatccaggt gacggaccgg gcgttcgacg acttcgccgg cagcgcgccc
     1081 gacgccggcc gcaatcggcg gctactgggc ctttagacca aaccttcgag gaagacgtcc
     1141 tgatgaagtt ccagcttttg ctcgtcgctg cattgtcggc catggcgacc agcgccgtgg
     1201 ccgcgccgta gggcccggac gtgttcgaac aacgctgcgc ggtctgccac tcgctgaagc
     1261 ccgcgccggg caagatgggg ccgaccctgg ctggcgtcgt tggccgcaag gccggaacgc
     1321 ttggcggcta tgcctacagt aacgccctga aggcctcggg catcacctgg aacgcccaga
     1381 cgctggacgc ctatgtcaaa gccccggcca agaccgtgcc ggggaccaag atgctgctcg
     1441 gcgcgcccga cgcccagcag cgcgcggccg tgatccagta cctggcttcg gtcaagaagt
     1501 agggcggggc atcctgcggg agcgctacac ctacaaaaac ttgtcatccc ggacgagcgc
     1561 gcagcgtgaa gatccgggac cgacgcgtga actcggtgct ccccgcggtc ccggatctgc
     1621 gcccggcttg cgccgagctt gtccgggatg acagcatctg gattccggac gcaacgctgc
     1681 gcctaggcga agctactgcg ccgtcagctc ctggatctcg atgtccttga attcgatggg
     1741 atggccctcg ctctgcaggg cgatatagcc ctccgtcacc ggctgggcgc cgcgcgccag
     1801 gatgtagggc ttggccccgc ccgcgacggt gtcgtcggga tccagggtca ggccggcata
     1861 gtggtggacc accaccccgt cgattttctg gaagacctcg ccattgggcc ggacctccag
     1921 ttcgaccttc gtccaggtcc catccggaat ggtcggcgca ttggccgggg gcgt
//
LOCUS       DAOMEO010000629         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6032_length_1972_cov_1.940532, whole genome shotgun sequence.
ACCESSION   DAOMEO010000629 DAOMEO010000000
VERSION     DAOMEO010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_1972_cov_1.940532"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="PLH31_13245"
     CDS             complement(<1..97)
                     /locus_tag="PLH31_13245"
                     /inference="COORDINATES: protein motif:HMM:NF018893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HQR90196.1"
                     /translation="MAYVDGFVLAVPKANIEAYKEMARLGAKVWME"
     gene            complement(192..1271)
                     /locus_tag="PLH31_13250"
     CDS             complement(192..1271)
                     /locus_tag="PLH31_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90197.1"
                     /translation="MTTRRVLLSALPLIGVASCGEGGDAYAQAVAATWVLPPASGAAG
                     LPVQALVQAATLAASGHNTQPWRFVSRGRELIIQPDLSRRTPVVDPDDHHLFVSLGCA
                     AETLLLAATGRGWSGEARFLGAAEGVAVNLSPGRQAPDPLCAAIARRASTRADYDPRP
                     VAIPSLRALAASAGPHVALSLITDRPRLDALTALIIDGDDVQVADPAFRRELAAWIRF
                     DAAEALARRDGLFSRSSGSPALPRWLGSRMFDLAFTVKGEADKIARQMANTAGAAVFT
                     GPSDDPAGWVAVGRAFTRFALTATALGLKLAFLNQPVEDRPTRARLAAWLGAPDRRPD
                     LVVRFGEGPAMPRSLRRPVSAVLSA"
     gene            complement(1354..>1972)
                     /locus_tag="PLH31_13255"
     CDS             complement(1354..>1972)
                     /locus_tag="PLH31_13255"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HQR90198.1"
                     /translation="QVMIEGPGHVPMHKIKANMDEQLKHCHEAPFYTLGPLTTDIAPG
                     YDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGVITYKLAAHAADLAKGH
                     PGAAMWDDAISRARFEFRWEDQFNLALDPETARKFHDETLPKEAHKTAHFCSMCGPKF
                     CSMEISHEVREFAAAQAPNEAAALGMAQMSEKFREQGSEIYLKTE"
BASE COUNT          313 a          666 c          702 g          291 t
ORIGIN      
        1 gctccatcca gaccttggcg cccagcctgg ccatctcctt gtaggcctcg atattggcct
       61 tggggaccgc gagtacgaaa ccgtcgacat aggccatcgg gaaatcctcc ttatcctgtt
      121 gaggatacca gtacgtttcg gcgacgatgg atccgacacc ctcgcccctg aaatcgccag
      181 accgcggacg ttcaggccga gagcacggcc gaaaccggcc ggcgcaggga ccttggcatg
      241 gcgggcccct cgccgaaacg aaccaccaga tcgggccgcc ggtcgggcgc gccaagccag
      301 gcggcgaggc gcgcgcgggt gggccggtcc tcgacaggct ggttgagaaa ggccagcttc
      361 aggcccagcg ctgtggcggt cagggcgaaa cgggtgaagg cgcggccgac cgcgacccag
      421 ccggccggat cgtcggaagg tcccgtaaac accgccgccc cggcggtatt ggccatctga
      481 cgggcgatct tgtcggcctc gcccttgacg gtgaacgcca gatcgaacat ccgcgagccc
      541 agccagcgcg gcagggccgg gctgccggag ctgcgcgaga acagcccgtc gcggcgagcc
      601 agggcctcgg cggcgtcaaa ccggatccac gccgccagtt cgcggcggaa ggccggatcg
      661 gccacctgaa cgtcatcgcc gtcaatgatc agcgcggtca gcgcgtcaag gcgcgggcga
      721 tcggtgatca ggctcagcgc cacgtgaggt ccggcggagg ccgccagggc gcgcaagctc
      781 gggatcgcca ccggacgcgg gtcatagtcg gcgcgggtgg aggcgcgacg ggcgatcgcc
      841 gcgcacaggg gatcaggcgc ctgacgcccc ggtgacaggt tgacggccac cccctcggcg
      901 gcccccagga acctcgcctc gcccgaccag ccccggccgg tggcggccag caaaagggtc
      961 tcggccgcgc agcccaggct gacgaacagg tggtggtcgt cgggatcgac aaccggcgtg
     1021 cgccgcgaca ggtccggctg gatgatcagc tcccgcccgc gcgagacgaa gcgccagggc
     1081 tgggtgttgt ggccgctcgc cgccagggtc gcggcttgga ccagcgcctg gacaggcagt
     1141 cccgccgccc ccgaagccgg cggcaggacc caggtggcgg ccacggcctg ggcataggcg
     1201 tcgccgccct ccccgcaact ggcgacgccg atcagcggaa gcgcggacag caggacgcgg
     1261 cgggtggtca tcagagggct ccttcagaga tcctgtgatg cggtcaacgc gtcagcctga
     1321 ccttgagatg ggtcaaggcc aggccagagt cccctactcg gtcttcagat agatctccga
     1381 gccctgctcg cggaacttct cgctcatctg cgccatgccc agggccgccg cctcgttcgg
     1441 ggcctgagcc gcggcgaact cccgcacctc gtggctgatc tccatcgagc agaacttggg
     1501 tccgcacatc gagcagaagt gcgcggtctt gtgcgcctcc ttgggcaggg tctcgtcgtg
     1561 gaacttgcgg gcggtttccg ggtcgagcgc caggttgaac tggtcttccc agcggaactc
     1621 gaaccgcgcc cggctgatgg cgtcgtccca catggccgcg ccggggtgcc ccttggccag
     1681 gtcggcggcg tgggcggcca gcttgtaggt gatgacgccg gtcttgacgt cgtcgcggtc
     1741 gggcagaccc aggtgttcct tgggcgtgac gtagcagagc atggccgtgc cgaaccagcc
     1801 gatcatcgcc gcgccgatcg ccgaggtgat gtggtcgtag ccaggggcga tgtcggtggt
     1861 caacgggccc aaggtgtaga agggggcctc gtggcagtgc ttgagctgct catccatatt
     1921 ggccttgatc ttgtgcatgg gcacgtggcc cggcccttcg atcatcacct gc
//
LOCUS       DAOMEO010000630         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6035_length_1971_cov_2.971294, whole genome shotgun sequence.
ACCESSION   DAOMEO010000630 DAOMEO010000000
VERSION     DAOMEO010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_1971_cov_2.971294"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLH31_13260"
     CDS             <1..1102
                     /locus_tag="PLH31_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQR90199.1"
                     /translation="SALLEVLDPAQNSAFGDHYLEVDYDLSQVMFVTTANSLNMPQPL
                     LDRMEIIRIAGYTEDEKLEIAKRHILPKLTKDHGLKEAEFVVPEKAIRDLIRYYTREA
                     GVRSLERELGALARKTVRDLAREKLASITIDDERLAKYAGVKKYRYGETDEVDQVGIV
                     TGLAWTEFGGDILTIEAVKMPGKGRMTVTGNLKDVMKESISAAQSYVRSRAMHFGVKP
                     PIFEKTDVHIHVPDGATPKDGPSAGAAMAVAMVSVLTGIPIRKDIAMTGEITLRGRVT
                     AIGGLKEKLLAALRSGVKTVLIPQENEKDLVDVPQSVKDGLEIIAVSTVDDVLKHALT
                     GPLTPVEWNEADEPIAASAKVDEGDADAILTH"
     gene            1252..1530
                     /locus_tag="PLH31_13265"
     CDS             1252..1530
                     /locus_tag="PLH31_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR90200.1"
                     /translation="MTTKAELVTAIAEKAGINKNQAKDALEAFIESVTDSLKSGNDVR
                     LVGFGTFKAVARAAGTARNPRTGETVNRPASKTARFQVGEGLKTTLNG"
     gene            1638..1712
                     /locus_tag="PLH31_13270"
     tRNA            1638..1712
                     /locus_tag="PLH31_13270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1670..1672,aa:Val,seq:tac)
     gene            complement(1717..>1971)
                     /locus_tag="PLH31_13275"
     CDS             complement(1717..>1971)
                     /locus_tag="PLH31_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR90201.1"
                     /translation="MAGAPLLCLPMGRDQNDNAARVVARGAGLRLAPDASVEAITAAI
                     AELIENNSYRQAAGLLGQTIRFDQTHRSAATLLEDLLVRD"
BASE COUNT          381 a          605 c          635 g          350 t
ORIGIN      
        1 ctcggccctg ctcgaggtgc tggaccctgc gcagaactcg gcgttcggcg accactatct
       61 tgaggtggac tacgacctca gccaggtgat gtttgtcacc acggccaaca gcctcaacat
      121 gccccagccc ctgctggacc gcatggagat cattcgcatc gccggctata ccgaggatga
      181 gaagctggag atcgccaagc gtcacatcct gcccaagctg accaaggacc acggtctcaa
      241 agaggctgag ttcgtggtgc ccgagaaggc gatccgcgac ctgatccgct actacacgcg
      301 tgaagctggc gtgcggtcgc tcgagcggga actgggggcg ttggcgcgga agaccgtccg
      361 cgatctggcg cgtgagaagc tcgcttcgat caccatcgac gacgagcggc tggccaaata
      421 tgcgggcgtc aagaaatacc gctacggcga gaccgacgag gtcgatcagg tcggtatcgt
      481 cacgggtctg gcctggaccg aattcggcgg cgacatcctg accatcgaag ccgtgaagat
      541 gccgggcaag ggccgcatga cggtcaccgg caacctcaag gacgtgatga aggagtcgat
      601 ctcggcggcc cagtcttatg tccgctcgcg ggccatgcac tttggtgtca agccgccgat
      661 ctttgaaaag accgacgttc acatccacgt gccggacggc gcgacgccca aggatggccc
      721 ttcggccggc gccgccatgg ctgttgccat ggtttcggtg cttacgggca tccccatccg
      781 caaggacatc gccatgaccg gcgagatcac cttgcgcggc cgggtcacgg cgatcggggg
      841 cctcaaggaa aagctgctgg ccgccctgcg ctcgggcgtg aagaccgtgc tgatccctca
      901 ggagaacgag aaggatctgg tcgacgtgcc gcagtcggtc aaggacggcc tggaaatcat
      961 tgcggtctcc acggtcgatg atgtcctgaa gcacgccctg acgggcccgc tgacgcccgt
     1021 tgagtggaac gaggcggacg agccgattgc tgcaagtgcg aaggtcgacg agggcgatgc
     1081 tgacgccatt ctgacccatt gaacgtaagt tattgttgat aagttgagcg gcgcgggaca
     1141 ttcccgcgcc gccttcattt gacggggcat tttccgccgc cataatcccg gccaagcgaa
     1201 gtggcgtcgg cttttgcgaa gcgccgccgc tttacaggct gggagaaaaa catgaccaca
     1261 aaagccgaac tcgtcacggc gatcgccgag aaggcgggca tcaataaaaa tcaggctaag
     1321 gacgccctgg aagccttcat cgaatcggtc accgactctc tgaagtcggg caatgacgtg
     1381 cgcctcgtcg gcttcggcac gttcaaggcc gtggcccgag cggcgggcac tgcgcgcaat
     1441 ccgcgcaccg gcgaaactgt gaaccgtccg gcctccaaga ccgcgcggtt ccaggttggc
     1501 gaaggcctga agaccacgct caacggctga tctgtttttc ggaaatggga gggcggagcg
     1561 cttcgcagcg cgccgccctt ttcttttcac gctttgcacg gaccctgccg gcggctagaa
     1621 ggccggctcg acttttgggg cggttagctc agcggtagag cgtctccttt acacggagag
     1681 ggtcgggggt tcgatcccct caccgcccac cagaactcag tctctcacga gcaggtcttc
     1741 cagcagggtc gccgcagacc ggtgggtctg gtcgaaacgt atcgtctggc ccagcaggcc
     1801 ggcggcctga cgatagctgt tattctcaat cagctctgcg atcgcggcgg tgatcgcctc
     1861 gacgcttgcg tcgggtgcaa ggcgtagtcc ggcacctctg gcgacgacac gggcggcatt
     1921 gtcattctga tcgcgcccca tgggcaggca gaggagtggc gcgccagcca t
//
LOCUS       DAOMEO010000631         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6042_length_1969_cov_2.497910, whole genome shotgun sequence.
ACCESSION   DAOMEO010000631 DAOMEO010000000
VERSION     DAOMEO010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_1969_cov_2.497910"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLH31_13280"
     CDS             <1..400
                     /locus_tag="PLH31_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HQR90202.1"
                     /translation="AEGFQVMVYCSDDPVYCRKLEDAGAVAIMPLGAPIGSGLGIQNR
                     VNLRIIIENAGVPVLVDAGVGTASDAAIGMELGCDAILMNTAIAEAKDPIRMARAMKH
                     AVIAGREAYLAGRMPKRMYADPSSPLAGLI"
     gene            complement(397..1137)
                     /locus_tag="PLH31_13285"
     CDS             complement(397..1137)
                     /locus_tag="PLH31_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR90203.1"
                     /translation="MILPRRAGALAASLALSGMLLSACTPAKPDKDFGEKVRAYLLEH
                     PEVLLEVSAKLEQKQAAKTAEAGRKALSQYRQALERDPRDFVANPNGSITVTEFFDYK
                     CGYCRVAAPEVVKLIAENPDVRFVFKEFVIFGNDSEAAARLMIGAKDQGKTLQLHQAL
                     MAEKSLDEASALRIARSLGLDVAKARAVGASAATTQHLADIRGLAQSLAIEGTPAFIV
                     GDTIIPGADMQALKLAIANTKAAALKKS"
     gene            complement(1164..>1969)
                     /locus_tag="PLH31_13290"
     CDS             complement(1164..>1969)
                     /locus_tag="PLH31_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR90204.1"
                     /translation="YQEVFDQARRFAYFRSHPLSSQRIETLRSRVERQSHYDAVDSPA
                     DLAEHQIMKAKLEGFLNPQVALMKYKESDNSFPARYARAIAYYQMKEPDRALKLIDSL
                     LVDNVDNPYLWELKGQVLFEFNRVEQAEEPQRRSVALKPDAPLLRINLGQTLINQKEP
                     AKVEEGVRELKRALLNDTDNAVTWRLLAQAYDKQGKDGDARLATAEQYFSMGAAQEAR
                     VFAMRARELLTKDTPEWRRATDIVLASRPSNQDLKDLAKEGATQSVFRR"
BASE COUNT          314 a          596 c          678 g          381 t
ORIGIN      
        1 cgccgagggc ttccaggtga tggtctattg ctcggacgac ccggtctatt gccgcaagct
       61 ggaagatgcc ggcgccgtcg cgatcatgcc gctgggcgcg ccgatcggct cggggctggg
      121 catacagaat cgcgtcaacc tgcggatcat catcgagaac gcaggcgtac cggtgctggt
      181 cgacgccggc gtgggcaccg cctcggacgc cgcgatcggc atggaactgg gctgcgacgc
      241 catcctgatg aacaccgcca tcgccgaggc caaggacccg atccgcatgg ctcgggcgat
      301 gaagcatgcg gtcattgcgg gccgggaagc ctatcttgcg gggcggatgc ccaagcggat
      361 gtacgccgat ccgagctcgc cgctggcggg gctgatctag ctcttcttca gcgccgccgc
      421 cttggtgttg gcgatggcga gtttcagcgc ctgcatgtca gcgccgggga tgatcgtatc
      481 tccgacgata aaggcaggcg tgccctcgat cgccagggat tgagccagtc cgcgaatatc
      541 ggccagatgc tgcgtcgtcg ccgctgacgc gccaacagcc cgcgccttgg cgacgtccag
      601 tcccaggctc ctggcgatac gcagggcact ggcctcgtcg aggctcttct cggccatcag
      661 ggcctgatgc aattgcagtg tcttgccctg gtccttggcg ccgatcatca gccgggccgc
      721 tgcttcggaa tcgttgccga agatgacgaa ctccttgaat acgaagcgta cgtcgggatt
      781 ttcggcgatc agcttgacga cttcaggcgc cgccacccgg cagtagccgc atttgtagtc
      841 gaagaactcg gtgacggtga tcgagccatt tggattggcc acgaagtcgc gcggatcgcg
      901 ctcaagagcc tgacggtatt ggctcagcgc cttgcggccg gcctccgctg tcttcgcggc
      961 ctgcttctgc tctagcttgg cgctgacttc cagcagcacc tcgggatgct ccagcagata
     1021 ggcccggacc ttttcgccaa agtccttatc gggcttggcc ggtgtgcagg cggaaaggag
     1081 catgcccgac agggcgagcg aagcggcgag ggcgccggcg cggcggggca ggatcatgag
     1141 ggtctcgaaa atcggggtgc tgatcagcgg cggaagacgg actgggtcgc gccttccttg
     1201 gccagatcct tgaggtcctg gttgctgggg cgcgaggcca ggacgatatc ggtggcgcgc
     1261 cgccattcgg gcgtgtcctt ggtcaggagc tcgcgagcgc gcatggcgaa cacccgggct
     1321 tcctgagcgg cgcccatgga gaagtactgc tcggcggtgg ccagacgggc gtcgccgtcc
     1381 ttgccctgct tgtcataggc ctgggccagc aggcgccagg tgaccgcgtt gtcggtgtcg
     1441 ttgagcaggg cgcgtttcag ctcgcgcacg ccttcctcga ccttggcggg ctctttctga
     1501 ttgatcaggg tctggcccag attgatgcgc agcagagggg cgtcgggctt gagcgcaacg
     1561 gagcggcgct gaggctcctc ggcctgctcg acccggttga actcgaacag gacctggccc
     1621 ttcagttccc aaagataggg gttgtcgaca ttgtcgacca gcaggctgtc gatcagcttc
     1681 agggcccggt cgggctcctt catctggtaa taggcgatgg cgcgggcgta gcgtgcgggg
     1741 aagctgttgt cgctttcctt gtacttcatc aacgcgactt gcgggttgag gaagccctcc
     1801 agcttggcct tcatgatctg gtgttcggcc agatccgccg gactatcgac agcatcgtag
     1861 tgcgactggc gctcgacccg gctgcgcagg gtctcgatcc gttgcgatga cagggggtgg
     1921 ctgcggaaat aggcgaagcg gcgcgcctga tcgaagacct cctgatagc
//
LOCUS       DAOMEO010000632         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6043_length_1969_cov_2.474399, whole genome shotgun sequence.
ACCESSION   DAOMEO010000632 DAOMEO010000000
VERSION     DAOMEO010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_1969_cov_2.474399"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..418
                     /locus_tag="PLH31_13295"
     CDS             236..418
                     /locus_tag="PLH31_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HQR90205.1"
                     /translation="MTRFIKNFLNDESGATAIEYGLIVALIAVVIVTAVTTLGTNLKT
                     TFTNVGTKVSTANSAS"
     gene            576..>855
                     /locus_tag="PLH31_13300"
     CDS             576..>855
                     /locus_tag="PLH31_13300"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HQR90206.1"
                     /translation="MQVLQLPLLLIFPALVIVAALKDVTSYTIPNWISLGLIAAFIPA
                     AAVSGASLPAIGLCLATGFGALLLGMGMFAAGWIGGGDAKLFAASALWI"
     assembly_gap    856..865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <866..1095
                     /locus_tag="PLH31_13305"
     CDS             <866..1095
                     /locus_tag="PLH31_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein CpaA"
                     /protein_id="HQR90207.1"
                     /translation="PAAFPFMLSTGLAGGALTMTILALRSGWLEPVLAGSPSWLRKLS
                     AQGGDIPYGVAIAVGALATFPQGALALAILG"
     gene            1300..>1969
                     /gene="cpaB"
                     /locus_tag="PLH31_13310"
     CDS             1300..>1969
                     /gene="cpaB"
                     /locus_tag="PLH31_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HQR90208.1"
                     /translation="MNPVRLLIVLVAAVSAIGLALVMQRALGGKPAAAPSAIAAPVSG
                     KPMTQVLVAKRDLAIGARLTGEDVTWQAWPTEAVNAAFITNGAALPKPEGAAKAAATV
                     SNAANEMIGGVDPAKAIEGAIVRDPILTGEPITPRKIVRGGDGGYLSVVLSPGKRAMS
                     VPVTSETAVGGFILPGDRVDVLQTRETAGRGEDGGSSKMVIAETVLQNVRVLALDQST
                     AAEKD"
BASE COUNT          328 a          689 c          597 g          345 t
ORIGIN      
        1 gaccacgttc accaacgtcg gcaccaaggt cagcacggcc aactcggcca gctaaatcct
       61 ggcttcaggc ctcgaaaaaa gaagggtcgg aacctcggtt ccggcccttt ttctttgttc
      121 ttctgtcact gccaaattta tttgagaaat cgaataatat tcatcgttga aaacggttga
      181 tttacctgga taaggcaaag tcattctcac gagtttcaac atcggagatt gacccatgac
      241 ccgtttcatc aagaacttcc tgaacgacga atccggcgcc acggccattg aatatggcct
      301 gatcgtcgcc ctgatcgccg tcgtgatcgt gaccgccgtc accacgctgg gcaccaacct
      361 gaagaccacg ttcaccaacg tcggcaccaa ggtcagcacg gccaactcgg ccagctaagc
      421 gcgcggggcc cgcaaggcct caagcacgac ctcgaaaacg ggagggccgc gacggtttcg
      481 tcgcggcctt ttccgtgttc gccttttcgt atttggggga aaccattgct tcacctgatc
      541 gtcccagaat ggggcgttca acaaggcgct ccccgatgca agtcctccag ctcccgctgc
      601 tgctgatctt tccggccctg gtcatcgtgg ccgcgctgaa ggacgtcacc agctacacca
      661 tccccaactg gatctccctg ggcctgatcg cggccttcat cccggcggcg gccgtcagcg
      721 gcgcgtccct gccggccatc ggcctgtgcc tcgccaccgg ctttggcgcc ttgctgctcg
      781 gaatgggcat gttcgcggcc ggctggatcg gcggcggcga cgccaagctg tttgcggcca
      841 gcgccctgtg gatcgnnnnn nnnnnggccg gcggctttcc ccttcatgct gtcgaccggt
      901 ctggcgggcg gcgccctgac catgaccatc ctggccctgc gctcgggctg gcttgagccg
      961 gtgctggccg gaagcccttc ctggctgcgc aagctgagcg cccagggcgg cgacattccc
     1021 tatggcgtgg ccatcgcggt cggcgccctg gccaccttcc cgcagggcgc cctggcgctg
     1081 gccattctgg gctgagcccc cgggggactg agccagtccc gcgtcagcct gtcacagcgg
     1141 gcatatcgac tcacggaccc aaaactttag ccggtgttaa ccatgcccgg gcgagcatga
     1201 cgcgggccag aaaggctgtc tgacgaatcg tgcgcgccgt cctgaagggc cgttgctcgc
     1261 gaaacctcgc ttctcgtcgc gaaagccagt gaccgcgcaa tgaatcctgt ccgcctcttg
     1321 atcgtcctgg tcgccgccgt ttcagccatt ggcctggccc tggtgatgca gcgcgcgctg
     1381 ggcggaaagc ccgccgccgc gccgtcagcc atcgccgcgc ccgtctcggg caagccgatg
     1441 acccaggtgc tggtcgccaa gcgcgacctg gccatcggcg cgcgcctgac cggcgaggac
     1501 gtcacctggc aggcctggcc gaccgaggcc gtcaacgccg ccttcatcac caatggcgcg
     1561 gccctgccca agcctgaagg cgccgccaag gccgccgcca ccgtctccaa cgccgccaac
     1621 gagatgatcg gcggcgtcga tccggccaag gctatcgagg gcgcgatcgt gcgcgacccg
     1681 atcctgaccg gcgaacccat caccccccgc aagatcgtgc gcggcggcga cggcggctat
     1741 ctgtcggtcg tgctgtcgcc cggcaagcgc gccatgtcgg tgcccgtcac ctccgaaacc
     1801 gccgtcggcg gcttcatcct gcccggcgac cgggtcgacg tgctgcagac ccgcgagacc
     1861 gcgggtcgcg gagaagacgg cggcagcagc aagatggtga tcgccgagac cgtcctgcag
     1921 aatgttcgcg ttctggccct cgatcagagc accgccgccg agaaggacg
//
LOCUS       DAOMEO010000633         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6044_length_1969_cov_2.350052, whole genome shotgun sequence.
ACCESSION   DAOMEO010000633 DAOMEO010000000
VERSION     DAOMEO010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_1969_cov_2.350052"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1345
                     /locus_tag="PLH31_13315"
     CDS             14..1345
                     /locus_tag="PLH31_13315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR90209.1"
                     /translation="MIVIGSGTAAQVAIAKVRAAGWSVAVIDHLPFGGTCALRGCDPK
                     KMLVSGEEAIDAAQRMRGHGVQGELSIDWPELMAFKRTFTDPIPAKQEDRYADLGIDS
                     FHGLASFADPETIQVDGRRLMARHILIATGARAIPLGIPGADLVSTSDDFLELPSLPR
                     RIVLIGGGYIAAEFSHLAARAGSEVVILQRGPRLLPHFDADIVGWLTPRFRELGITVE
                     TGVSVSAVEQNADDLTVHGTRRDGPDVQVSADLVVHAAGRGPDLEALNLAAGGVAVKE
                     GRLLLNGHLQSTSNPRVYAAGDAAGVGPPLTPVSSHDGKVVAANLLGGPAREPDYRGV
                     PSVAFTVPPIASVGMDEAAAHRSGRRFRVNAASTPDWFSARRLAECIYGHKVLIDEDT
                     DLILGAHLVGPQADEVINIFGLAIRHGLTAADVRSTMFAYPTGASDVGYML"
     gene            complement(1652..>1969)
                     /locus_tag="PLH31_13320"
     CDS             complement(1652..>1969)
                     /locus_tag="PLH31_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR90210.1"
                     /translation="DNRRQVAAYAGLAPTPWKSGSIDHEQGVAKAGNPRLRTTMIQVA
                     WLWTRHQPTSALSQWFFERVARNGGRMRKSNIVALARKLLVAFWKYVSSGVVIEGAVM
                     QAT"
BASE COUNT          318 a          663 c          632 g          356 t
ORIGIN      
        1 ggacgcctac gacctgatcg tcatcggcag cggaacggct gcgcaggtcg ccatcgcaaa
       61 ggtccgcgct gccggctggt cggttgcggt aatcgaccat cttccgttcg gcggcacctg
      121 cgcgctgcga ggctgcgatc ccaagaagat gctggtcagc ggcgaagagg ccatcgatgc
      181 tgcgcaacgc atgcgcgggc atggagtcca gggcgagctg tcgatcgact ggccagaact
      241 gatggccttc aagcgaacgt tcaccgatcc gatccctgca aagcaggaag atcgatacgc
      301 cgacttgggg atcgattctt tccacgggct tgccagcttc gcggaccccg agacgatcca
      361 ggtggacggg cgcaggctga tggcccgcca catcctgatc gcgaccgggg cacgcgccat
      421 cccgctgggc attcctgggg cggatctggt ttccacaagc gacgatttcc ttgagctgcc
      481 gagccttcca cggcgcatcg tcctgatcgg gggcggatac attgcggccg aattttccca
      541 cctcgccgcg cgcgctggct cagaggtcgt gatcctgcag cgaggcccaa ggctcctgcc
      601 gcacttcgac gccgacattg ttggatggtt gactccgcgc ttccgtgagc tcgggatcac
      661 cgtcgagacc ggtgtgagcg tttccgccgt tgagcagaac gccgacgacc tgactgtcca
      721 cgggacgcgg cgcgacggac cggacgtcca ggtttccgct gacctggtgg tccacgcggc
      781 gggccgcggc cccgacctcg aggcgttgaa tctcgccgcc ggcggggttg cggtgaagga
      841 aggacgcctg ctgctcaacg gacatctcca gagcacctcc aacccgcgag tctacgccgc
      901 tggcgacgcc gccggggtgg ggccaccgct cacgccagtc tccagccacg acggcaaggt
      961 ggtcgccgcc aatctgcttg gcgggcccgc gcgcgagccg gactatcgcg gcgtaccgag
     1021 cgtggccttt acagtgccgc cgatcgcgtc cgttgggatg gacgaggccg ctgcacatcg
     1081 cagcgggcgc cgcttccgcg tcaacgccgc ctcaacgccg gactggttca gcgcacgccg
     1141 cttggccgag tgcatctacg gccacaaggt gctgatcgac gaggataccg acctgatcct
     1201 cggcgcccat ctcgtgggac ctcaggccga cgaggtcatc aacatcttcg ggttggcgat
     1261 ccgccacggc cttacagcgg ccgacgtccg gtccacaatg ttcgcctatc cgaccggcgc
     1321 ctcggacgtc ggctacatgc tctgacggct ggggcgtcgc atccggacgc tccagcattg
     1381 tcgcctggcc aaggctagca gcgtgtggcg agcctcatcg gagtttcaga atccgcggtc
     1441 tgagccgtat ccgcgacctg gcctcaacca gatcaacctc acattcggtg gccgcgttgt
     1501 ggcggcgaac caatttcccg cattcgatgg cggcggctca ggaggacggg accattcttc
     1561 atctgcggct ggttgaaggc cacggctgca atcggttcgt ccacctggat ccctcaggac
     1621 tgatcaggcc cttgggatag ggtggtcgtt gtcaggtggc ctgcatgacg gctccctcga
     1681 taacaacgcc gctgctgacg tacttccaga acgccaccag gagcttccgg gccagcgcca
     1741 cgatgttgct cttgcgcatc cgaccgccgt tgcgtgccac ccgctcgaag aaccactggc
     1801 tcagggccga tgtgggctga tgccgtgtcc acagccacgc cacttggatc atcgtcgttc
     1861 tcaggcgagg attgccggct ttcgccacgc cctgctcatg gtcgattgat ccgcttttcc
     1921 agggcgtcgg ggccaggccg gcgtaggccg ccacctgtcg ccgattatc
//
LOCUS       DAOMEO010000634         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6065_length_1961_cov_2.846275, whole genome shotgun sequence.
ACCESSION   DAOMEO010000634 DAOMEO010000000
VERSION     DAOMEO010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6065_length_1961_cov_2.846275"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLH31_13325"
     CDS             complement(<1..408)
                     /locus_tag="PLH31_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90211.1"
                     /translation="MDFGIAGRRALVCASSSGLGKACAISLSGEGVEVWINGRDAERL
                     EAAAVEIEALTGRRPHTFVADIDTAEGRATALAACPEPDILVCNSGGPKPGRLADWTE
                     TAWSDVVAAKMISPILLIQTVIGGMRQRGFGRVV"
     gene            complement(429..1559)
                     /locus_tag="PLH31_13330"
     CDS             complement(429..1559)
                     /locus_tag="PLH31_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR90212.1"
                     /translation="MRKVFRDDHEMFRDQVRRFVETEIVPHHAQWERDGIVPKSLWLR
                     AGEQGLLCVTAPEEYGGSGGDFGHSAILIEELARVNATAVGFTTHSDIAVPYLVNYGT
                     DEQKARWLPRLITGDLIAVIAMSEPQIGSDLRGMKTKAVRDGDHYVLSGQKTFITNGI
                     NSGLAIVAAKVEGQANPKDITLFCVEEGTPGFDKGKKLEKVGLRGQDTSELFFSDVRV
                     PVENRLGEESKGFGCLMTELAKERLIIGIRAAASLESMLDKTIAYTKDRIVFGKPLFE
                     FQNTRFKLAEVKARSQMLRVFIDDCLQKLFDGELTPETAAMAKLTSAELHGQLLDELL
                     QLHGGYGYMSEYDVGRAWVDARVARIYAGTSEIMKEIIARTL"
     gene            complement(1573..>1961)
                     /locus_tag="PLH31_13335"
     CDS             complement(1573..>1961)
                     /locus_tag="PLH31_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR90213.1"
                     /translation="SFVKLGIVPGDGGAWLLPRIVGMPRASHMALTGDALNAQTALDW
                     TLVTQLTEPDALITTAMALAHRVASSPGHALRLTKRLLREGQHMRLGSLLELSAAYQA
                     LAHHTSDHAEAVAAMIEKRPAVYTDS"
BASE COUNT          307 a          631 c          660 g          363 t
ORIGIN      
        1 gaccacgcgg ccgaaacccc tctggcgcat gccgccgatc accgtctgga tcagcaggat
       61 cggcgagatc atcttggcgg cgacgacgtc gctccaggcg gtctcggtcc aatccgccaa
      121 tcggccgggc ttgggaccgc cgctgttgca gaccaggatg tcgggctcgg ggcaggcggc
      181 caaggccgtt gcacgtcctt cggcggtgtc gatgtcggcg acgaacgtgt ggggtcgtcg
      241 cccggtcagg gcctcgatct cgacggcggc tgcctccagg cgctcggcgt cccggccgtt
      301 gatccacacc tcgacgcctt cgccgctcag tgaaatggcg caggcctttc caaggcccga
      361 cgacgaggcg cagacgagcg cgcgccttcc ggcgatgccg aaatccatga gcctgctcca
      421 gatcgccctc agagcgtgcg ggcgatgatt tccttcatga tctcggacgt gccggcgtag
      481 atgcgggcga cgcgcgcatc gacccaggcg cgtccgacat cgtattcgct catataaccg
      541 tagcccccgt gaagctggag gagttcgtcc agcaactggc cgtgcaactc ggcgctggtc
      601 agcttggcca tggcggcggt ctcgggcgtc agttcgccgt cgaacagctt ctgcaggcag
      661 tcgtcgatga agacgcgcag catctggctt cgcgccttca cttcggccag cttgaaccgg
      721 gtgttctgga actcaaagag cggcttgccg aagacgatgc gatccttggt gtaggcgatg
      781 gtcttgtcga gcatcgactc cagcgaggcg gcggcgcgga ttccgatgat cagtcgctct
      841 ttggcgagtt ccgtcatcaa gcagccgaag cccttgcttt cctcgcccag ccgattttcg
      901 accggcaccc gcacgtcgga gaagaacagc tcggaagtgt cctggccgcg aaggccgacc
      961 ttttccagct tcttgccctt gtcgaagccg ggcgtgcctt cttcgacgca gaacagggtg
     1021 atgtccttgg ggttggcctg gccctcgacc ttggccgcga cgatggcgag ccccgaattg
     1081 atgccattgg tgatgaaggt cttctggccc gacaggacat agtggtcgcc gtcgcggacc
     1141 gccttggtct tcatgccgcg cagatcgctg ccgatctggg gctcggacat ggcgatcacg
     1201 gcgatcaggt cgccggtgat caggcgcggc agccagcggg ccttctgttc atcggttccg
     1261 taattgacca gatagggcac ggcgatgtcg gagtgggtgg tgaagccgac ggccgtggcg
     1321 ttcacccgcg ccagttcctc gatcaggatg gcgctgtggc cgaagtcgcc gccggagccg
     1381 ccgtattcct cgggcgcggt gacgcacagc agaccctgct cgccagcgcg cagccacagc
     1441 gatttgggca cgatgccgtc gcgttcccac tgagcgtggt gagggacgat ctcagtctcg
     1501 acgaaccgcc ggacctggtc gcggaacatc tcatggtcgt cgcggaaaac cttgcgcatc
     1561 gacatcgctt ccttagctgt ccgtgtacac ggcggggcgt ttctcgatca tcgccgcgac
     1621 cgcttcggcg tggtccgagg tgtggtgggc cagggcctga taggccgccg acagttctag
     1681 cagcgatccc agccgcatat gctggccttc gcgcaacagg cgcttggtga ggcgcagggc
     1741 gtgaccgggg ctgctggcga cccgatgggc cagggccatc gccgtggtga tcagggcgtc
     1801 tggctccgtc agctgggtca ccagggtcca gtccagcgcg gtctgggcgt tcagggcgtc
     1861 gccggtcagg gccatgtgac tggcccgcgg catgccgacg atccggggca acagccaagc
     1921 cccgccgtcg ccgggaacga tgcccagctt gacgaaactc t
//
LOCUS       DAOMEO010000635         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6068_length_1961_cov_1.836306, whole genome shotgun sequence.
ACCESSION   DAOMEO010000635 DAOMEO010000000
VERSION     DAOMEO010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6068_length_1961_cov_1.836306"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLH31_13340"
     CDS             complement(<1..226)
                     /locus_tag="PLH31_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HQR90214.1"
                     /translation="MARLRILHETRYYYDNPVSFGPQRLLLRPRDSHALRIVEASMTL
                     FPPGETRWSYDANGNCVCLFQPQEQADAMRV"
     gene            310..1554
                     /locus_tag="PLH31_13345"
     CDS             310..1554
                     /locus_tag="PLH31_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HQR90215.1"
                     /translation="MSAMAVLDLKRKLAILSDAAKYDASCASSGAEKRNSLSGPEGRG
                     KGVGSTEGMGICHAYAPDGRCISLLKILLTNFCIYDCAYCINRVSSNAPRARFTVKEV
                     VDLTLNFYKRNYIEGLFLSSGVIQTGDYTMEEMVRVAKSLRLDHDFRGYIHLKLIPEA
                     SPELAAEAGLYADRVSINIELPQDESLTILAPEKDPAGIKRAMGRMRLSIEDHNEPAK
                     KAGKRKFATSQSTQLIVGADAARDSDILARSANLYGAYKLRRVYYSAFSPIPDAASRL
                     PSMRPPLMREHRLYQADWLMRFYGFTQGEIVEPGEDLDLTVDPKTAWALRHRGDFPVD
                     VNTAYRDTLLRTPGLGTRGVDRILSARRQTRLTLADVKRVCGSTKRAQPFIVTADWTP
                     GELTDAKGLKASLIVPEQLSLF"
     gene            1554..>1961
                     /locus_tag="PLH31_13350"
     CDS             1554..>1961
                     /locus_tag="PLH31_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4130 domain-containing protein"
                     /protein_id="HQR90216.1"
                     /translation="MRVVRLASEIDFDGWRRAGRALRAEGVPPEAVVWTVEYDLFADG
                     AAPAAPAKGAGPRFTVPQPFIDLAERVVLNRSNERFALLYTLLWRLEREPGLIDNPAD
                     PDLTRARDMAKGVSRAAHKMKAFVRFRLVEGVEP"
BASE COUNT          333 a          677 c          618 g          333 t
ORIGIN      
        1 ccacgcgcat ggcgtcagcc tgctcctggg gctgaaacag gcacacgcaa ttgccgttgg
       61 cgtcgtagct ccagcgggtc tcgcccggtg ggaacagcgt catcgaggcc tcgacgatcc
      121 gcagggcgtg gctgtcgcgc gggcgcagca gcaggcgctg cggcccgaaa ctcaccgggt
      181 tgtcgtaata atagcgtgtc tcgtggagga tgcggagccg ggccattggc tcaagaaccc
      241 ccggcgtgtg cgcttcgttc catcgtcggc ccacgaagag catagacgga acatatcaag
      301 aactctatca tgtcggccat ggccgtcctc gatctgaagc gtaaactcgc catcctgtct
      361 gacgccgcca agtatgacgc ctcttgcgcc tcatccggag cggaaaagcg aaactccctt
      421 tccggccctg agggacgcgg caagggcgtg gggtccaccg agggcatggg catctgccac
      481 gcctatgcgc ccgacgggcg ctgcatctcc ctgctcaaga tcctgctgac caatttctgc
      541 atctatgact gcgcctattg tatcaaccgg gtcagctcca acgcgccgcg cgcgcggttc
      601 acggtcaagg aggtcgtcga cctcaccctc aacttctaca agcgcaacta tatcgagggt
      661 ctgttcctgt cgtcgggggt gatccagacc ggcgactaca cgatggaaga gatggtgcgg
      721 gtggccaaaa gtctgcgcct ggatcacgat tttcgcggct acatccatct gaaactgatc
      781 cccgaggcct cgcccgaact ggcggccgaa gccgggctct atgccgaccg ggtgtcgatc
      841 aatatcgaac tgccccagga cgaaagcctg acaatcctgg ccccggaaaa ggacccggcc
      901 ggcatcaagc gcgccatggg ccggatgcgg ctgtcgattg aggatcacaa cgaacccgca
      961 aaaaaggccg gcaagcgcaa gttcgccacc agccagtcga cccagttgat cgtcggggcc
     1021 gatgcggccc gcgacagcga catcttggcc cgcagcgcca acctctatgg cgcctacaag
     1081 ctgcggcggg tctattattc ggccttcagc ccgatccctg acgccgcctc gcgcctgccg
     1141 tccatgcggc cgccgctgat gcgcgagcac cgcctgtacc aggccgactg gctcatgcgg
     1201 ttctacggtt ttacgcaggg cgagatcgtc gagccgggcg aggacctgga cctgaccgtc
     1261 gatcccaaga ccgcctgggc cctgcgccat cgcggcgact tcccggtcga cgtcaatacc
     1321 gcctatcgcg acaccctgct tcgcacgccc ggcctgggga cgcggggcgt cgaccgcatc
     1381 ctgtcggccc gccgccagac ccgcctgacc ctggccgacg tcaagcgggt ctgcggttcg
     1441 accaagcgcg cccagccctt tatcgtcacc gccgactgga cgccggggga gctgaccgac
     1501 gccaagggcc tgaaggcgtc gctgatcgtg ccggagcagt tgagcctgtt ctgatgcgcg
     1561 tcgttcgtct ggcctccgag atcgacttcg acggctggcg ccgggccggc cgggccctgc
     1621 gcgccgaagg cgtgccgccc gaggcggtgg tctggacggt ggagtatgat ctgtttgccg
     1681 acggcgctgc cccggccgcg ccggccaagg gcgccggccc gcgcttcacc gtgccccagc
     1741 cctttatcga tctggccgaa cgggtcgttc tcaaccgttc caatgagcgc tttgccttgc
     1801 tctacacgct gctgtggcgg ctggagcgcg agccgggcct gatcgacaat ccggcagacc
     1861 ccgacctgac ccgcgcccgt gacatggcca agggcgtcag ccgcgccgcg cacaagatga
     1921 aggccttcgt ccgcttccgt ctggtcgagg gcgtcgagcc c
//
LOCUS       DAOMEO010000636         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6073_length_1960_cov_2.556955, whole genome shotgun sequence.
ACCESSION   DAOMEO010000636 DAOMEO010000000
VERSION     DAOMEO010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_1960_cov_2.556955"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLH31_13355"
     CDS             complement(<1..1002)
                     /locus_tag="PLH31_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR90217.1"
                     /translation="MYDAEKAPEVLDEAALRALEALYCSYGDTVHYLPDPKFFDGCEG
                     SFMYDSQGRQFLDLQMWYSAVNFGYRNPRLNAVAHRQLDTLPQVASQYLHREKVELAA
                     MIAQDAEKKFGRKGRVHFNVGGAQAVEDSLKLVRNASNGKQLMFAFEGGYHGRTLGAS
                     AITSSYRYRRRFGHFSDRAQFIEFPYHFRGPKGMSKEEYGAACVAKFARLFETEYNGV
                     LDTKTGQAEFAAFYIEPVQGTGGYVIPPPNFFIELKKVLDQHGILLVVDEIQMGMYRT
                     GKLWSIEHFGVQPDVLVFGKALTNGLNPLSGVWAREELINPEIFPPGSTHSTFNSNPM
                     "
     gene            complement(1015..1692)
                     /locus_tag="PLH31_13360"
     CDS             complement(1015..1692)
                     /locus_tag="PLH31_13360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HQR90218.1"
                     /translation="MRIFCDFDGTIAKVDTTDLVLTRLADPAWEALEARWTQGEITAA
                     ECMRGQVALIGGADAALDAVLDSVELADGFAEFVAWCQANAVPLTVVSDGVDRFIARI
                     LDRHGLGYLPVIANHLVGNAELGRRLEQPWSRVGCAAGSGVCKCQVATKSDERANKPD
                     DLMVFIGDGRSDFCVSGRADLLFARDKLADYARARAMPHYEFSDFSIITTTLTAALGR
                     SARRAAI"
BASE COUNT          354 a          605 c          641 g          360 t
ORIGIN      
        1 catggggttg gagttgaagg tcgaatgggt cgagccgggc gggaagattt ccgggttgat
       61 cagctcttcg cgcgcccaga cccccgacag cgggttcagg ccattggtca gggctttacc
      121 gaacaccagc acgtcgggct gtacgccgaa gtgctcgatc gaccaaagct tgccggtccg
      181 gtacatgccc atctgaattt catcgaccac cagcaggatg ccgtgctggt cgagaacctt
      241 cttcagttcg atgaagaagt tgggcggcgg aatgacatag ccgccagtgc cctggacggg
      301 ctcgatatag aaggcggcga attcggcctg gccggtcttg gtgtcgagca cgccgttata
      361 ttcggtctcg aacagccggg cgaacttggc cacgcaggca gcgccgtact cttccttgct
      421 catgcccttt gggccacgga agtggtatgg gaattcaatg aattgggcgc ggtcgctgaa
      481 gtggccgaag cgccgacgat agcggtagga cgaggtgatc gccgaggcgc ccaaggtgcg
      541 gccgtgatag ccgccctcga aggcgaacat cagctgtttg ccattgctgg cattgcgcac
      601 cagcttgaga gagtcctcga ccgcctgggc gccgccgacg ttgaagtgga ccctgccctt
      661 gcgaccgaac ttcttctcgg cgtcctgagc gatcatcgcc gccagttcga ccttttcgcg
      721 gtgcagatat tggctggcca cctgcggcag ggtgtcgagc tgacggtggg cgacggcgtt
      781 caggcgcggg ttgcggtagc cgaaattgac ggccgagtac cacatctgaa ggtccaggaa
      841 ctggcgaccc tggctgtcgt acatgaacga cccttcgcat ccgtcgaaga acttggggtc
      901 gggcagatag tggacggtgt cgccatacga acagtacagg gcctcaagcg cgcgcagggc
      961 ggcctcgtcc aggacttcgg gggctttttc agcgtcgtac atgtggggct ccggtcaaat
     1021 ggcggcgcgg cgcgccgaac ggccaagagc ggccgtgagc gtcgtcgtga tgatgctgaa
     1081 gtcggaaaat tcgtaatgag gcatggctcg cgcgcgggcg tagtcggcca gcttgtcgcg
     1141 ggcgaacagc aggtcggcgc ggcccgagac gcaaaagtcc gagcgcccgt caccgatgaa
     1201 gaccatcagg tcgtcgggct tgttggcgcg ctcgtcagac ttggtggcga cttggcattt
     1261 gcagacgcca gaccccgcag cacagccgac gcgcgaccaa ggttgctcca ggcgtcgccc
     1321 cagttcagcg ttgccgacca gatggttggc gatcaccggc aggtatccca ggccatggcg
     1381 atccaaaatc cgggcgatga aacggtccac cccgtcgctg accacggtca gcggcacggc
     1441 attggcttgg caccacgcca cgaactcggc aaagccatcg gccaactcga cgctgtccag
     1501 cacggcgtcc agcgccgcgt cagcgccgcc gatcagggcg acctgtccgc gcatgcactc
     1561 ggcggcggtg atctcgccct gggtccagcg cgcctcgagc gcttcccagg ccgggtcggc
     1621 cagacgggtc aggacaaggt cggtggtgtc gaccttggcg atcgtcccgt cgaaatcgca
     1681 aaatattcgc atctgtttga cttggctcca gacgcccccg cgtcgttcga gccaatgacg
     1741 ccttgtaggt tcaggcccct caccgattgt cctgtcgcga tacccagccg aaagcggggc
     1801 gatctcggct tgccttcatc ccgccggcca gccatgtgtt tttggggtca acgactcccg
     1861 caatgagccg attgtcctgc cgatgagcac caccccctcc acgacgatcc ttggcgagac
     1921 acggcctagc gcccgtgcga tcggctgaga tggacggctt
//
LOCUS       DAOMEO010000637         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6076_length_1959_cov_2.475315, whole genome shotgun sequence.
ACCESSION   DAOMEO010000637 DAOMEO010000000
VERSION     DAOMEO010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_1959_cov_2.475315"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLH31_13365"
     CDS             <1..936
                     /locus_tag="PLH31_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQR90219.1"
                     /translation="DDALDWARALDAPGTYAPYPRPAPTPPVEDRPRKMAVTRIEALT
                     RDPYAVWARDILKLYPLERPDEPVEARARGTAIHAAFEKFSQAHPGPVPANAPAIFEA
                     LYMSELVSAGMPPTALARERALAREAAAWVADWERERRAKAEQIIVEAEGQLTLPING
                     NLFTLTAKADRIEPTRDGLAHILDYKTGAAPSKKQVETGFSPQLTLTAAILLNGGFPD
                     LQGRKPGDLTYVRVTGRKPAGLEEVRVAAADSEHAAVQALDGLRALITRYDDPSQPYR
                     SRTAPQFVKDHPGDYAHLARVFEWSTSGDDGEGSE"
     gene            1064..>1959
                     /locus_tag="PLH31_13370"
     CDS             1064..>1959
                     /locus_tag="PLH31_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR90220.1"
                     /translation="MTPDPQRLAADPAISAFVTANAGSGKTKTLIDRVARLLLAGSTP
                     EAILCVTYTKAAAAEMQRRLFERLGGWSVTADSPLRAELARLVGQPEETFGPAELSKA
                     RALFARALETPGGLKIQTIHAFCEKLLRRFPLEAGVSPGFVVMDDSASSAVARAARRT
                     VATYVDTHDDAFAQAYARFSVALDFAAFESMFGTFEIRRGAIADYMARHGGLSGAIED
                     VWRVCGFDEPTDPQAVAAEAMDELDPDLWEACASLLESSGKARDTEHAARLRVTLASR
                     DFDDALRALFTDKEEPRKHLQTT"
BASE COUNT          309 a          735 c          639 g          276 t
ORIGIN      
        1 gacgacgccc tggactgggc ccgcgccctc gatgcgcccg ggacctacgc gccctatccc
       61 cgccccgccc cgaccccgcc ggtcgaggat cgtccgcgca agatggccgt gacccggatc
      121 gaggccctga cgcgcgaccc ctacgccgtc tgggcccgcg acatcctcaa gctttatccg
      181 cttgagcgcc ccgacgagcc ggtcgaggcc cgggcgcgcg gcaccgccat ccacgcggcc
      241 ttcgagaagt tctcgcaggc gcatcccgga ccggttccag caaacgcccc ggccatcttc
      301 gaggcgctct atatgagcga gctggtgtca gccggcatgc cgccgacggc cctggcccgc
      361 gaacgcgccc tggcccggga agccgccgcc tgggtcgccg actgggagcg ggagcgccgc
      421 gccaaggccg aacagatcat cgtcgaggcc gaagggcagc tgaccctccc catcaacggc
      481 aaccttttca ccctgaccgc aaaggccgac cgcatcgagc cgacccgcga cgggctggcc
      541 catatcctcg actacaagac cggcgccgcg ccgtcgaaaa agcaggtcga gaccggcttc
      601 tcgccgcaac tgaccctgac ggcggcgatc ctgctgaacg gcgggttccc tgacttgcag
      661 ggccgcaagc ccggcgacct gacctatgtg cgggtcaccg gccgcaaacc ggcgggcctg
      721 gaggaggtgc gtgtcgccgc cgccgacagc gagcacgccg ccgtccaggc gctggatggt
      781 ctgcgcgccc tgatcacccg ctatgacgat ccgtcccagc cctaccgctc gcgcaccgcg
      841 ccgcagttcg taaaagacca tcccggcgac tacgcccatc tggcgcgggt gtttgaatgg
      901 tcgaccagcg gcgacgatgg ggagggttcg gagtgagggc gctcagcgag atcctccccc
      961 tctgggggag gtggcccaga gggccggagg gggccagcac ggccgacctc cagctagccc
     1021 cctccgtcgc ctgcggcgac acctccccca gagggggagg attatgaccc ccgaccctca
     1081 acgcctagcc gccgatccgg cgattagcgc cttcgttacc gccaatgccg gctcgggcaa
     1141 gaccaagacc ctgatcgacc gggtggcgcg gctgctgctg gctggatcga cacccgaggc
     1201 gatcctgtgc gtgacctaca ccaaggccgc cgccgccgag atgcagcggc ggctgttcga
     1261 gcggctgggc ggctggtcgg tgacggccga tagtcccctg cgggccgagc tggccaggct
     1321 ggtcggccaa cccgaggaaa ccttcggccc ggcagagctt tccaaggctc gcgccctgtt
     1381 cgcccgcgct ctggaaacgc ccggcggcct gaagatccag accatccacg ccttctgcga
     1441 aaagctgctg cggcgctttc cactggaggc cggggtctcg cccggcttcg tggtgatgga
     1501 cgactcggcc agctcagccg tcgcccgcgc cgcccgccgc accgtggcga cctatgtcga
     1561 cacccatgac gacgccttcg cccaggccta tgcgcgcttt tcggtggcgc tggatttcgc
     1621 agccttcgag tcgatgtttg ggaccttcga gatccggcgc ggcgcgatcg ctgactacat
     1681 ggctcggcac ggaggcctct ctggcgcgat cgaggatgtc tggcgcgtct gcggcttcga
     1741 cgagcccacc gatccacaag ccgtcgccgc cgaggcgatg gacgagctgg acccggacct
     1801 ttgggaagcc tgcgccagcc tgctggaatc cagcggcaag gcccgcgaca ccgagcacgc
     1861 cgcccggttg cgcgtcaccc tggccagccg cgatttcgac gacgccctgc gcgccctgtt
     1921 caccgacaag gaagagcctc gcaagcacct gcagacgac
//
LOCUS       DAOMEO010000638         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6082_length_1958_cov_2.866001, whole genome shotgun sequence.
ACCESSION   DAOMEO010000638 DAOMEO010000000
VERSION     DAOMEO010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_1958_cov_2.866001"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /gene="secA"
                     /locus_tag="PLH31_13375"
     CDS             complement(<1..1667)
                     /gene="secA"
                     /locus_tag="PLH31_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQR90221.1"
                     /translation="MLGFAKKLFGSSNDRKVKTLTARVAKINAFEPEYAALSDEALKA
                     KTAEFKARLEKGETLDDLLNEAFAVVREASKRVLGMRHFDVQLVGGMVLHFGGISEMR
                     TGEGKTLVATLPTYLNALSGKGVHVITVNDYLAKRDAEWMGQIYNFLGLSYGVIVNGL
                     SQGERQRAYRSDITYGTNNEFGFDYLRDNLVYSVEEMVQRGHHFVIVDEVDSILIDEA
                     RTPLIISGPTEDRSELYKTIDVLVKELIKDKSNYDHDEKQKQVILTEDGQERIEEILI
                     AGGHLAEDTAGLYDAANISLVHHVNQALRANVLYTRDKDYIVKGDEVILIDEFTGRMM
                     TGRRLSEGLHQAIEAKEGADIQPENQTLASVTIQNYFRLYTKLSGMTGTASTEAQEFE
                     DIYKMGVSEIPTNRPVQRIDDDDEVYRTEAEKNQSILKQIADCHMRGQPILVGTVSIE
                     KSEELSKLLSTYAWEHDGKKHNGIPHQVLNARFHEQEAGIVADAGAPGAVTIATNMAG
                     RGTDIQLGGNLDMRLASWRQQQRGLGITPLPADELAMRAEIEADIAES"
BASE COUNT          303 a          578 c          659 g          418 t
ORIGIN      
        1 gactcggcga tgtcggcctc gatctcggcg cgcatggcca gttcgtcagc gggcagcggg
       61 gtgatgccca ggcccctctg ctgctggcgc cagctggcca ggcgcatgtc gagattgccg
      121 cccagctgga tgtcggtgcc gcggccggcc atgttggtgg cgatagtcac cgcgccgggg
      181 gcgcccgcgt ctgcgacgat gccggcttcc tgttcgtgga agcgggcgtt cagcacctgg
      241 tgcggaatgc cgttgtgctt cttgccgtca tgctcccagg cataggtcga cagcagcttt
      301 gacagttcct cggacttctc gatcgacacg gtgccgacca ggatcggctg gccgcgcatg
      361 tggcagtcgg cgatctgctt gaggatcgac tggttcttct cggcttcggt gcgatagacc
      421 tcgtcgtcgt cgtcgatgcg ctggaccggg cggttggtcg ggatttccga cacgcccatc
      481 ttgtagatgt cttcgaattc ctgagcctcg gtcgaggccg tgccggtcat gcccgacagc
      541 ttggtgtaga ggcggaaata gttctgaatg gtcaccgagg ccagggtctg gttctcgggc
      601 tggatgtcgg cgccttcctt ggcctcgatg gcctggtgca ggccttccga caggcggcgg
      661 ccggtcatca tgcgaccggt gaactcgtcg atcaggatca cctcgtcgcc cttgacgatg
      721 tagtccttgt cgcgcgtgta cagcacgttg gcgcgcaggg cctggttcac gtggtggacc
      781 agcgagatgt tggcggcgtc gtacaggccg gcggtgtcct cggccaggtg gccgcccgcg
      841 atcaggatct cttcgatacg ctcctggccg tcctcggtga ggatgacctg cttctgcttt
      901 tcgtcgtgat catagttcga cttgtccttg atgagctcct tcaccaggac gtcgatggtc
      961 ttgtacagct cgctgcggtc ctcggtgggg cccgagatga tcagcggggt gcgggcttcg
     1021 tcgatcagga tcgagtcgac ctcgtcgacg atcacgaagt ggtggccgcg ctggaccatt
     1081 tcctcgacgc tgtagacgag gttgtcgcgc aggtagtcga agccgaattc gttgttggtg
     1141 ccgtaggtga tgtcgctgcg ataggcgcgc tgacgctcgc cctggctcag accgttgacg
     1201 atcacgccat acgacaggcc caggaagttg tagatctgac ccatccactc ggcgtcgcgc
     1261 ttggccaggt agtcgttcac ggtaatgacg tggacgcctt tgcccgacag ggcgttcagg
     1321 taggtgggca gggtggcgac cagggtcttg ccctcgccgg tgcgcatttc cgagatgccg
     1381 ccaaagtgca gcaccatgcc gcccaccagc tggacgtcga agtggcgcat ccccagcacg
     1441 cgcttggagg cttcgcgaac aacggcgaag gcttcattca ggagatcatc gagggtttcg
     1501 cccttttcca ggcgcgcctt gaactcggcg gtcttggcct tcagggcctc gtcggagagc
     1561 gcggcgtact ccggctcgaa agcgttgatc ttcgccacgc gggcggtcag cgtcttgacc
     1621 ttgcggtcgt tggaagagcc gaagagcttt ttggcgaagc cgagcatgaa gtcgaaattc
     1681 cgatgaagtg ttaggcgctg cgggcgtcgg ggttgctgcg aggtgacatc caggcacgac
     1741 taatccggcc cttacggcgc gcgcgagact taagcagcga ccctcccagt gtcaacgcga
     1801 ggccgtctta tagagcacgg gcaaggcgaa ggtttccggc cttttccgcg tttagtcggc
     1861 aggtgcgcca aaaatgccgg aaagccttcc caaggatgcg ccggagttgc attcccctgc
     1921 ggctgaaacc gcgctagagc cgtccagaag gttcgtcg
//
LOCUS       DAOMEO010000639         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6086_length_1957_cov_2.172976, whole genome shotgun sequence.
ACCESSION   DAOMEO010000639 DAOMEO010000000
VERSION     DAOMEO010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_1957_cov_2.172976"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLH31_13380"
     CDS             <1..568
                     /locus_tag="PLH31_13380"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HQR90222.1"
                     /translation="HCLADLVYRWKIGELPMDIAGVVSNHPAETYAHVDLSGIPFHHL
                     PVTKETKFEQEAELWKLIQESKTDIVVLARYMQVLSDGLSAKLAGRCINIHHSFLPGF
                     KGAKPYHQAHARGVKVIGASAHYVTGDLDEGPIIEQDVERISHRDTPEDLIRKGRDIE
                     RRVLARALRYRLEDRVLLNGRKTVVFTD"
     gene            639..1703
                     /gene="rfbB"
                     /locus_tag="PLH31_13385"
     CDS             639..1703
                     /gene="rfbB"
                     /locus_tag="PLH31_13385"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQR90223.1"
                     /translation="MSRNLRIMVTGGCGFIGSAVCRYLAGLGDAAILNFDAMTYAASE
                     ASLADLRGHNSYAFVKGDVADASAVAEAIAEFKPTHIMHLAAESHVDRSITGPGAFIQ
                     TNVIGTYVMLQAALEYWRTLDSQAAQAFRFHHISTDEVFGSLGAEGLFSETTPYDPRS
                     PYSASKASSDHMVRAWHHTYGLPVVVSNCSNNYGPYHFPEKLIPLVTLNALEGKPLPV
                     YGKGDNVRDWLYVDDHAKALVLIATKGVVGESYNVGGRNERTNLEVVEAICDLLDELR
                     PQAGLKRRDLITFVHDRPGHDARYAIDATKLETELGWKAEENFDTGLRKTVEWYLARE
                     DWWRPLRERYDGQRLGLKTA"
     gene            1703..>1957
                     /locus_tag="PLH31_13390"
     CDS             1703..>1957
                     /locus_tag="PLH31_13390"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQR90224.1"
                     /translation="MSSPRIALFGANGQLGQDLSSIASARGLTLIALQRPMFDATDAA
                     GLDAALADLDFDVAINSVAVTRVDDCEKDPAPAVAINAYFA"
BASE COUNT          347 a          671 c          602 g          337 t
ORIGIN      
        1 ccactgcctg gctgatctgg tctatcgctg gaagatcggc gagctgccga tggatatcgc
       61 cggggtggtc tccaaccacc cggccgagac ctatgcccat gtcgatctgt cgggcatccc
      121 cttccatcac ctgcccgtga cgaaagagac caagttcgag caggaagccg agctttggaa
      181 gctgatccag gagagcaaga ccgacatcgt cgtcctcgcc cgctacatgc aggtattgtc
      241 cgacggactg tcggccaagc tggccgggcg ctgcatcaac atccaccact cgttcctgcc
      301 gggcttcaag ggcgccaagc cctatcacca ggcccatgcg cgcggcgtga aggtgatcgg
      361 ggccagcgcc cactatgtga ccggggacct cgacgagggc ccgatcatcg agcaggacgt
      421 cgagcgcatc agccaccgcg acacgcccga agacctgatc cgcaagggcc gcgatatcga
      481 gcgccgcgtg ctggcccgcg ccctgcgcta tcgccttgag gaccgggttt tgctgaacgg
      541 ccgcaagacg gtggtcttta ccgactaagc ttggcgggtt gcgccagcag ggatttctaa
      601 gctacggggc gggcttaagt cgtttccgga acctgggcat gtctcgcaat cttcgtatca
      661 tggtcaccgg cggctgcggg ttcatcggct cggcggtctg tcgctatctg gcaggcctgg
      721 gcgacgcggc gatcctcaat ttcgacgcca tgacctatgc ggcctccgag gccagcctgg
      781 ccgacctgcg cggccacaac agctatgcct tcgtgaaggg tgatgtcgcc gacgcttcgg
      841 cagtcgccga agccatcgcc gagttcaagc cgacccacat catgcacctg gcggcggaat
      901 cccacgtcga tcgctcgatc accggcccgg gcgccttcat ccagaccaat gtcatcggca
      961 cctatgtgat gctgcaggcc gcgctggagt actggcgcac cctggacagc caggccgcgc
     1021 aagccttccg cttccaccac atctcgaccg acgaggtttt cggcagcctg ggcgccgagg
     1081 gtctgttctc cgagaccacg ccctacgatc cgcgctcgcc ctattcggcg tcgaaggcct
     1141 cgtccgacca catggtccgc gcctggcatc acacctatgg cctgccggtg gtggtctcca
     1201 actgctccaa caattacggc ccctatcact tccccgagaa gctgatcccg ctggtcacgc
     1261 tgaacgccct cgagggcaag ccgctgccgg tctatggcaa gggcgacaat gtccgcgact
     1321 ggctctatgt cgacgaccac gccaaggcgc tggtgctgat cgccacaaag ggcgtggtcg
     1381 gcgagagcta taatgtcggc ggtcgcaatg agcggacaaa cctcgaagtc gtcgaagcca
     1441 tctgcgacct gctggacgag ctgcgcccgc aggcggggct gaagcgccgc gacctgatca
     1501 ccttcgtcca cgaccgtccg ggccacgacg ctcgctacgc catcgacgcc accaagctgg
     1561 aaaccgagct gggctggaag gcggaggaaa acttcgacac cggcctgcgc aagaccgtcg
     1621 aatggtatct ggcgcgcgag gactggtggc ggccgctgcg cgagcgctat gacggacagc
     1681 gcctgggact gaagaccgcc tgatgtcttc tccccggatc gcgctgtttg gcgccaatgg
     1741 ccagttgggt caggacctct cgtccatcgc ctcggcgcgc ggcttgaccc tgatcgccct
     1801 gcaacgcccg atgttcgacg cgaccgacgc cgcgggcctc gacgcggccc tcgccgatct
     1861 cgacttcgac gtcgccatca acagcgtcgc ggtcacgcgc gtggacgact gcgagaaaga
     1921 cccggcgcca gccgtggcga tcaacgccta ttttgcc
//
LOCUS       DAOMEO010000640         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6089_length_1956_cov_3.488690, whole genome shotgun sequence.
ACCESSION   DAOMEO010000640 DAOMEO010000000
VERSION     DAOMEO010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6089_length_1956_cov_3.488690"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLH31_13395"
     CDS             <1..748
                     /locus_tag="PLH31_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQR90225.1"
                     /translation="AVLEDLGDDLYARLIEAGTDEAPLYDAAIDGLLALHAAPTPGVL
                     EFDGSTWPLLVYDDLALKTAHDIFIEWLPKFRPVTFDANTLAEWEAIWSPIRSLGEAG
                     ATVFCHRDYHAENLIWLPERQGAARVGMLDFQDAVLAHPAWDLSMLLHDARRTVSPER
                     EAACLDRYLAARPELDRIAFLADYHALGALNIARILGIFARLVTRDGKPRYEQFMPRL
                     WTYLDVCLADPALARLKAWFDIYVPAEARR"
     gene            745..1461
                     /locus_tag="PLH31_13400"
     CDS             745..1461
                     /locus_tag="PLH31_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR90226.1"
                     /translation="MSAPKIAMVLAAGLGTRMRPLTDDRPKALVEVGGRVLIDHMLDR
                     LVEAGVETAVVNVHAFADRLQAHLMARQARGLGPRIVISDERPQALETGGGIRNALPL
                     LGDGPVWVANIDSVWIESGAPALDAVAAAWDPAGMDVCLLLASTAESLGFHDSGDVFL
                     EDGVVRFKGPGEMAPLVYVGVHICKPGITADGPDGPFSLTPIWKNLAAKGRVHGVSPE
                     GMWMHVGDPGARDLAEARLK"
     gene            1458..>1956
                     /locus_tag="PLH31_13405"
     CDS             1458..>1956
                     /locus_tag="PLH31_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HQR90227.1"
                     /translation="MTFGGPFEREGPRWFSIPAHRPFVDDLARGFLAALSHMGPEALP
                     RATLLTPTRRGARAVADALLAASDGKALLLPQIRPLGDLDEGEPPFEPGDLALDLPPA
                     ISSRRRRFELARIVVDHADLLNFTPGASQALEMAKALSDFLDSCRIEEIDTDGKLDGL
                     ADGDLA"
BASE COUNT          271 a          707 c          659 g          319 t
ORIGIN      
        1 ggccgtgctg gaagaccttg gcgacgacct ctatgcccgg ctgatcgagg ccggaaccga
       61 cgaggccccg ctctatgacg ccgccatcga cgggctgctg gccctgcatg ccgcgccgac
      121 tccgggggtg ctggagttcg acggctcgac ctggccgctg ctggtctatg acgacctggc
      181 cctgaagacc gcccacgaca tcttcatcga atggctgccg aagttccgcc ccgtgacctt
      241 cgacgccaac actcttgcgg aatgggaagc catctggagc ccgatccgca gcctcggcga
      301 ggctggcgcc acggtgttct gtcatcgcga ctatcacgcc gagaacctga tctggctgcc
      361 ggagcgccag ggcgcggcca gggtcggaat gctggacttc caggacgcgg tgctggctca
      421 cccggcctgg gacctgtcga tgctgctgca cgacgcacgc cgcaccgttt cgcccgagcg
      481 cgaagccgcc tgcctggatc gctatctggc ggctcggccg gagctggatc gcatcgcctt
      541 cctggccgac tatcatgcgc tgggcgcgct caacatcgcc cggatcctgg gcatcttcgc
      601 ccgcttggta acccgcgacg gcaagccccg ctatgagcag ttcatgccgc gcctctggac
      661 ctatctggac gtctgcctcg ccgatccggc cctggcgcgc ctgaaggcct ggttcgacat
      721 ctacgtcccc gccgaggctc gccgatgagc gcgccgaaga tcgcgatggt gctggccgcc
      781 gggctgggca cccgcatgcg gccgctgacc gatgatcggc ccaaggccct ggtcgaggtc
      841 ggcggccggg tgctgatcga tcacatgctg gaccgcctgg tcgaggccgg ggtcgagacc
      901 gccgtggtca atgtccacgc cttcgccgac cggctgcagg cccatctgat ggcccgtcag
      961 gccaggggcc ttggtccccg tatcgtcatc tctgacgaac gcccccaggc gctggaaacc
     1021 ggcggcggga tcaggaacgc cctgcccctg ctgggcgatg gccccgtgtg ggtggccaat
     1081 atcgactccg tctggatcga gagcggcgcg cccgccctcg acgccgtcgc ggcggcctgg
     1141 gaccctgcgg ggatggatgt ctgtctgctg ctggcctcga cggccgagtc cctgggcttc
     1201 cacgacagcg gcgacgtctt cctcgaggat ggggtcgtgc gcttcaaggg gcccggcgag
     1261 atggcgccgc tggtctatgt cggcgttcat atctgcaagc cggggatcac ggccgatggc
     1321 ccagacgggc cgttctcact gaccccaatc tggaagaatt tggccgccaa gggccgcgtc
     1381 cacggcgtct cgcccgaggg gatgtggatg cacgtcggcg atcccggcgc ccgcgatctg
     1441 gcggaagcgc ggctgaaatg accttcggcg gcccttttga gcgcgagggg ccgcgctggt
     1501 tctcgatccc ggcccatcgc cccttcgtgg acgatctggc ccggggcttt ctggcggccc
     1561 tgtctcacat gggccccgag gccctgcccc gcgccaccct gctaaccccg acccggcgcg
     1621 gcgcccgcgc cgtggccgac gccctgctgg ccgccagcga cggcaaggcc ctgctgctgc
     1681 cgcagatccg gccgctgggc gacctcgacg agggcgaacc gccgtttgag ccgggcgacc
     1741 tggccctgga cctgccgccc gccatcagtt cgcgccgccg ccggttcgaa ctggcccgga
     1801 tcgtggtcga ccacgccgac ctgttgaatt tcacccccgg cgcctcgcaa gcgctggaga
     1861 tggccaaggc cctgtcagac ttcctcgaca gctgccggat cgaggagatc gacaccgacg
     1921 gaaagctgga cggcctggcc gacggcgacc tggccc
//
LOCUS       DAOMEO010000641         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6090_length_1956_cov_2.081010, whole genome shotgun sequence.
ACCESSION   DAOMEO010000641 DAOMEO010000000
VERSION     DAOMEO010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6090_length_1956_cov_2.081010"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLH31_13410"
     CDS             <1..867
                     /locus_tag="PLH31_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HQR90228.1"
                     /translation="ETVAGVLAQLKPVLAVLQRDGEGNIDHGAALAVLAPGAARNALD
                     CALWDLRAKLTGVSVAEATGLPVPASLVTAVTVSLDAPEAMAAAARAVADAPLIKIKL
                     SAEDPAARLRAVAAAAPNARFIVDPNEGWTFEILHGLRALLADLPIALVEQPLPADAD
                     GALDGYDPPYVICADESVHVGGDLAALRSRYQAVNLKLDKTGGFSEAVRMLEQARALG
                     FQLMTGCMVASSLGIAPALHLAGACQFADLDGPWWLAVDHPGGLRVEGGVITPPAAGF
                     WGDGVDAEGLWL"
     gene            complement(877..1344)
                     /locus_tag="PLH31_13415"
     CDS             complement(877..1344)
                     /locus_tag="PLH31_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90229.1"
                     /translation="MEFPRGRLDLYLTEIGEPADNDLRIVVLQADARGPIESTEVGEA
                     RRIMPAEASPAIELVWYDYVAYAMRSESYARLEDEEPLSKDSFTVKYDSAFLTYVRAS
                     TFATDEFPGPLTHWSLDTLNHCLDVVSTSPPEVRVLDVAERPRGPTSQNWAKN"
     gene            1521..>1956
                     /locus_tag="PLH31_13420"
     CDS             1521..>1956
                     /locus_tag="PLH31_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQR90230.1"
                     /translation="MTDPTPAKPGPLAWWFAIPLYQRILGALILGAVVGYVWGEGATS
                     LKIVGEVFIRLIRMLVAPLVFVIIATGVAELGDPKRLGGIGVKTISMYFLTVFLAVVV
                     GLICASLFQPGLGADFANVAPQAVGTPKSPAEIFIGIVPLNPI"
BASE COUNT          289 a          654 c          640 g          373 t
ORIGIN      
        1 gagaccgtgg cgggcgtcct cgcacagctg aaacccgttt tggccgtgtt gcaacgggac
       61 ggggagggca atatcgatca cggcgctgct cttgccgtgt tggctcccgg cgcagcgcgt
      121 aacgctctgg actgcgccct gtgggacctg cgggccaagc tcaccggcgt cagtgtggcc
      181 gaggccaccg gcctgccggt cccggcttca ctggtgaccg ccgtcaccgt cagcctggac
      241 gcgcccgagg ccatggccgc cgccgcccgg gccgtggccg acgcgccgct gatcaagatc
      301 aagctctcgg ccgaagaccc cgccgcccgc ctgcgcgccg tggccgccgc cgcgccaaac
      361 gcccgcttca tcgtcgatcc caatgagggc tggacgttcg agatcctcca cggcctgcgc
      421 gccctgctgg ccgacctgcc gatcgccctg gtcgagcaac ccctgccggc cgacgccgat
      481 ggggcgctgg acggctatga cccgccctac gtgatctgcg ccgatgagtc ggtgcatgtg
      541 ggcggcgatc tcgccgcgct gcgcagccgc tatcaggccg tgaacctcaa gctggacaag
      601 acgggcgggt tttccgaggc ggtgcggatg ctggaacagg cccgggcgct gggtttccag
      661 ctgatgaccg gctgcatggt ggcctcgtcg ctgggcatcg ccccggccct gcatctggcc
      721 ggcgcctgcc agttcgccga cctcgacgga ccgtggtggc tggcggtgga tcaccccggc
      781 ggtttgcggg tcgagggcgg tgtgatcacg ccgccggccg ccgggttctg gggggatggt
      841 gtcgatgccg aggggctgtg gctctaactg cctctgctag ttcttggccc aattctggct
      901 tgtcggccca cgcggtcgtt ccgcgacgtc cagaactcgt acttccggcg gcgacgtact
      961 gacgacgtcc aagcagtggt tcagcgtatc caggctccaa tgcgtgagag ggccaggaaa
     1021 ctcgtcggtc gcgaaagttg atgctcttac ataggtcaga aaagccgagt cgtatttgac
     1081 agtgaagctg tccttgctga gtggttcttc gtcttcgaga cgcgcatagc tttcgctgcg
     1141 catggcatag gcgacatagt cgtaccaaac gagttcgatg gctgggctag cttcggctgg
     1201 cattatgcgc cgcgcctcgc caacctccgt gctctcgatg ggccctctag catccgcctg
     1261 tagcaccacg atccgaaggt cattgtccgc aggttctcca atctcggtga gatagaggtc
     1321 aaggcgtccc ctcgggaatt ccataaaagc atctccacac aatctagtgc gacgctagag
     1381 gcgcgactgt cgctcatcaa catggccgcg ctccattggg tggcggtcgg ggtcaggccc
     1441 ctcgtcagta agccatccag acttgaccat cgccgcgacg cgccatgctc tggacgcctc
     1501 agtctgaccg gacccttcgc atgaccgacc cgacacccgc caaaccgggc cctttggcct
     1561 ggtggttcgc catccccctc tatcaacgta ttctcggcgc cctgatcctc ggcgcggtcg
     1621 tcggctatgt ctggggcgag ggcgcgacca gtctgaagat cgtgggcgag gtgttcatcc
     1681 ggctgatccg gatgctggtg gcgccgctgg tgttcgtgat catcgccacc ggcgtggccg
     1741 agctgggcga tcccaagcgg ctgggcggca tcggggtcaa gaccatctcg atgtacttcc
     1801 tgaccgtgtt cctggccgtc gtggtgggcc tgatctgcgc gagcctgttc cagccgggcc
     1861 tgggcgccga cttcgccaat gtcgccccgc aggcggtggg cacgcccaag tcgccggccg
     1921 agatctttat cggcatcgtg ccgctgaacc ccatca
//
LOCUS       DAOMEO010000642         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6098_length_1954_cov_2.849394, whole genome shotgun sequence.
ACCESSION   DAOMEO010000642 DAOMEO010000000
VERSION     DAOMEO010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6098_length_1954_cov_2.849394"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>358
                     /locus_tag="PLH31_13425"
     CDS             <1..>358
                     /locus_tag="PLH31_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQR90231.1"
                     /translation="PHSLTLTLVGAALLWVGWFGFNAGSNLESNAYASLAMINTLVAT
                     AAAGLSWILVEWVTRKKPSALGLASGVVAGLVAITPAAGFAGPMGAVILGLVVSPICI
                     IFCSAVKNALKYDDSLD"
     assembly_gap    359..368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <369..855
                     /locus_tag="PLH31_13430"
     CDS             <369..855
                     /locus_tag="PLH31_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQR90232.1"
                     /translation="ASGVVAGLVAITPAAGFANPQGALIIGIIGGGAAYVGAVWLKRL
                     LKYDDSLDAFGIHGIGGIVGAIGTGLVVNPAWGGAGVVDYTSCAKDGDISTCDTAVYS
                     LATQVMAQVKAVGVTIIWSAIASAIVFFLIKLVIGLRASPDAEEEGLDISEHGERAYH
                     S"
     gene            complement(937..1506)
                     /locus_tag="PLH31_13435"
     CDS             complement(937..1506)
                     /locus_tag="PLH31_13435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR90233.1"
                     /translation="MSQATDRLQVANNVRVDVETGQGKIVIELYVDRAPITTANFLRY
                     VDQKRYDGATFFRASRAPGPADYGLIQGGLQGDPARVLKPIAHEPTTQTGIKHTDGVV
                     SIARNAPGTATSDFFICVGDAPYLDADPNAPGDNAGFAAFGKVVEGMDVVRKIINLPT
                     PGVAVNPVMKGQILEPPVPILSARRAAAL"
     gene            complement(1644..>1954)
                     /locus_tag="PLH31_13440"
     CDS             complement(1644..>1954)
                     /locus_tag="PLH31_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQR90234.1"
                     /translation="PEGEARPWMPDAFESAEEIAAWLSAATRNDLEAPAVALEPLIGE
                     VLDVLRDEPETLLARMSGSGATCFALCAGDIEAEGLAERLESMRPSWWVRRCRLDRVD
                     "
BASE COUNT          305 a          667 c          624 g          348 t
ORIGIN      
        1 ccgcactcgc tgaccctgac ccttgtgggc gctgccctgc tgtgggtggg ctggttcggc
       61 ttcaacgccg gttccaacct ggaatccaac gcctacgcct cgctggccat gatcaacacc
      121 ctggtcgcca ccgccgccgc cggtctgtcc tggatcctgg tggagtgggt cacccgcaag
      181 aagccgtcgg ccctgggcct ggcttcgggc gtcgtggccg gcctggtcgc catcaccccg
      241 gccgccggtt tcgccggccc gatgggcgcc gtgatcctgg gcctggtggt ctcgccgatc
      301 tgcatcatct tctgctcggc cgtgaagaac gcgctgaagt acgatgacag cctggacgnn
      361 nnnnnnnngg cttcgggcgt cgtggccggc ctggtcgcca tcaccccggc cgccggtttc
      421 gcgaacccgc aaggcgctct gatcatcggc atcatcggtg gcggcgcggc ctatgtcggc
      481 gcggtctggc tcaagcgtct gctcaagtat gacgacagcc tggacgcctt cggcatccac
      541 ggcatcgggg gcatcgtcgg cgccatcggc accggcctgg tggtcaaccc ggcctggggc
      601 ggcgctggcg tcgtcgacta caccagctgc gccaaggacg gcgacatctc gacctgcgac
      661 accgctgtct acagcctggc cacccaggtg atggcccagg tcaaggccgt cggcgtgacg
      721 atcatctggt cggccatcgc ttcggcgatc gtcttcttcc tgatcaagct ggtcatcggt
      781 ctccgcgctt cgccggacgc cgaggaagaa ggtctggaca tctccgagca cggggaacgc
      841 gcctatcaca gctaagcccc ggctcagctg aaaaggcgaa gggcgcggag cttggctccg
      901 cgccctttct ttttgtctca gtatctggcg gagctgttac agcgcggcgg ctcggcgggc
      961 gctgaggatc ggcaccggcg gctccaggat ctggcccttc atgaccgggt tcaccgccac
     1021 gcccggggtc ggcaggttga tgatcttgcg caccacgtcc atgccctcaa ccaccttgcc
     1081 gaaggcggcg aagccggcat tgtcgcccgg cgcgtttgga tcggcatcca gatagggtgc
     1141 gtcacccacg cagatgaaga agtccgaggt cgccgtaccc ggcgcattgc gggcgatcga
     1201 gaccacgcca tcggtgtgct tgatcccggt ctgggtggtc ggctcatggg caatgggttt
     1261 gagcaccctg gccgggtcgc cctgcagccc gccctggatc agaccataat ccgcgggtcc
     1321 aggcgctctc gaggcccgaa agaacgtggc gccgtcataa cgcttctggt ccacatagcg
     1381 caggaagttc gccgtggtga tcggcgccct gtcgacatag agctcgatga cgatcttccc
     1441 ctgccccgtc tcgacatcga cgcggacgtt attcgccact tgcagcctat ccgtcgcttg
     1501 ggacatggtc tgaagcggtt gcgccaagac cggcaagccg attgaggctc cgataagggc
     1561 caataggtcg cgacgatgca tatgaaagct ccggatgaac tgaccagcag cctagtccgc
     1621 ccgtcgcgag ccgtcaccca agcttagtcg accctatcca gtcgacagcg cctcacccac
     1681 cagctgggcc gcatgctttc cagccgttca gccaggccct cggcctcgat atcaccggcg
     1741 cagagggcaa agcacgtggc gccagagccc gacatccgag ccagcagggt ctccggctcg
     1801 tcgcggagaa catccagcac ctcgccaatc agaggctcga gcgccacggc gggcgcttcc
     1861 agatcgttgc gggtggcggc gctcagccag gcggcgatct cctcggcgct ctcgaaggcg
     1921 tcgggcatcc agggcctggc ttcgccttca ggcg
//
LOCUS       DAOMEO010000643         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6107_length_1952_cov_1.872430, whole genome shotgun sequence.
ACCESSION   DAOMEO010000643 DAOMEO010000000
VERSION     DAOMEO010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6107_length_1952_cov_1.872430"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLH31_13445"
     CDS             complement(<1..198)
                     /locus_tag="PLH31_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90235.1"
                     /translation="MTSMWKAALLAGAAWGAAATAVSAQEAPVANAGLSVEEVVVTAR
                     RREESLKDVPVAVSAYSGETLA"
     gene            complement(400..1098)
                     /locus_tag="PLH31_13450"
     CDS             complement(400..1098)
                     /locus_tag="PLH31_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR90236.1"
                     /translation="MTAAKPRARAGGRGRPSKAKGLDLKETILDAAEGLFARHGFYGV
                     TTRQVAAEAGVDTALIHYYFGAKRELFDAVFLRRAEILNQARLASMDLYVKSHAGSLT
                     VEGVIEAFIEPLLRISQEGGQGWKNYFVLVAQVNNTPVWGGETMTRFFDPVVHRLVEA
                     LKAARPDAEYRDLYWCYQFLTGAMMLALSETGRIDSLSKGECHSSDLEAVRRRLFAYC
                     AAGFETVIGRAAKG"
     gene            complement(1190..1741)
                     /locus_tag="PLH31_13455"
     CDS             complement(1190..1741)
                     /locus_tag="PLH31_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HQR90237.1"
                     /translation="MDDQNMSMRRSFSGRRFAVPALAVVTLGILAASPGVLAAPARLF
                     QRFGFAPHAPDLSLIAAAPLAVQLHIAGALVALGIGTALLVGVKGNGVHKVLGWTWVV
                     AMALTAISSLFIRSINSGDFSFIHLLSGWTLIALPMGVFAIKRGRVAAHRRTMTGMFV
                     GGLLVAGLLALFPGRLLWHVFLG"
BASE COUNT          348 a          673 c          621 g          310 t
ORIGIN      
        1 ggccagggtc tcgccgctat aggccgagac ggcgaccgga acgtccttga gactttcctc
       61 gcgacgacgg gcggtgacga ccacctcttc aaccgacagg cctgcattgg cgacaggcgc
      121 ctcttgagcc gaaacggccg tcgcggccgc gccccatgcc gcgccagcca gcagcgcagc
      181 cttccacatc gaagtcattg ggtttcccct cccttgtacg ttccgcccac ccgaaccgga
      241 tcctttttgg cggacacgtg ctcggaaccc gcgaattcat cgaaccgcga ataccggcac
      301 tgtcactcag agaacgtcga gacgccagcc ttttttcatc aggtggtgaa atggcggcgg
      361 actgtgtctt tcgggccccg gcgtccgcca gcctggcgtt cagcccttcg ccgcgcgccc
      421 gatgacggtc tcgaaacccg ccgcgcagta ggcgaaaagg cggcggcgca ccgcctccag
      481 atcgctggaa tggcattcgc ccttcgacag cgaatcgatc cgcccggtct ctgacagggc
      541 cagcatcatc gcgccggtga gaaactggta gcaccagtag aggtcgcgat attcggcgtc
      601 gggacgcgcc gccttcaggg cctcgaccag acggtggacc accggatcaa agaaccgcgt
      661 catggtctct ccgccccaga ccggggtgtt gttcacctgg gccaccagga cgaaatagtt
      721 cttccagccc tgcccgccct cctgggagat gcgcagcagg ggctcgatga acgcctcgat
      781 caccccctcg acggtcagcg atccggcgtg gctcttcaca tagaggtcca tcgaagccag
      841 tcgcgcctgg ttgaggatct cggcccggcg caggaacacc gcgtcaaaca gctcgcgctt
      901 ggcgccgaaa taatagtgga tcagcgccgt atcgaccccg gcctcggcag ccacctgccg
      961 ggtggtgacg ccgtagaaac cgtgacgcgc gaacaggccc tcggcggcgt cgaggatggt
     1021 ttccttcagg tccaggccct tggccttgga gggtctgcca cgcccgccag cacgggcgcg
     1081 gggcttggct gcggtcattc gactctcttg cctgccttga ttgcgctgtt ctccggatgg
     1141 cccatccgag gcagcgtggc aagcaaagct cgacaaagaa gctgggcagt cagcccagga
     1201 acacgtgcca gagcaggcgg ccgggaaaga gcgccagcag gcccgccacc agcaggccgc
     1261 cgacgaacat gccggtcatc gtgcgccggt gcgccgcgac cctgccgcgc ttgatggcga
     1321 aaacgcccat gggcagggcg atcagggtcc agccgctgag caggtgaatg aagctgaaat
     1381 cgccggaatt gatgctgcgg atgaacagtg agctgatcgc ggtcagggcc atggccacca
     1441 cccaggtcca gcccagcacc ttgtgaacgc cgttaccctt cacgccaacc agcaaggccg
     1501 tgccgatgcc cagggcgacc agggcgccgg cgatgtgcag ttgaacggcc agcggcgcgg
     1561 cggcgatcag cgacaggtcc ggcgcatgcg gcgcaaagcc aaagcgctga aacagacgcg
     1621 caggtgcggc cagcacgccg ggactggccg ccaggatgcc cagcgtgacg accgccaggg
     1681 ccggaacggc gaaacggcgc cccgaaaagc tgcgccgcat tgacatgttt tggtcgtcca
     1741 tcttcatctc ccgaggcgac ggcgctgccc aagtccgtcg acgcctcatc cctggagtgg
     1801 ctcctctcgt gcctcaatcg ccgttgcgcc agtcaccgcc ccgccttcgt gagcggctgg
     1861 cggacgccgc tcacgcttcg cgaatggcgt tgaccagccc ggaagcgttg cggggctttc
     1921 tggtctcggc cttcgcgggc ctgttcctgg cc
//
LOCUS       DAOMEO010000644         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6111_length_1950_cov_2.434301, whole genome shotgun sequence.
ACCESSION   DAOMEO010000644 DAOMEO010000000
VERSION     DAOMEO010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6111_length_1950_cov_2.434301"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..303
                     /locus_tag="PLH31_13460"
     CDS             64..303
                     /locus_tag="PLH31_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQR90238.1"
                     /translation="MARVLLFGRLADQAGWRERAFEATSLTALRGLIATDDPELGDAL
                     GGPGVQVAVDKTLVRGEAALTAATEVAFLPPMSGG"
     gene            300..761
                     /locus_tag="PLH31_13465"
     CDS             300..761
                     /locus_tag="PLH31_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQR90239.1"
                     /translation="MTTGSKIILTAEPFEPGALLTRFCAGRAETGAVASFTGLARAEQ
                     GQAAALELEAYPGFTDTAISDFAEAARTRFHLHDYLVVHRTGRIAPGEAIVFVATAAG
                     HRREAFEACDFLMDWLKSRAPFWKKEHGPDGARWIEPTDRDRTDAERWDQE"
     gene            765..1319
                     /gene="moaB"
                     /locus_tag="PLH31_13470"
     CDS             765..1319
                     /gene="moaB"
                     /locus_tag="PLH31_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HQR90240.1"
                     /translation="MSAAGLTPGGGIKPDLPFTPVRVAILTISDTRDEESDTSGQVLV
                     DRVKAAGHELGGRAVVRDDIPQIRDQVRNWIAGGQVDAIVTTGGTGLTGRDVTVEALE
                     PLFNKKIDGFSVVFHMVSYASVGLSTLQSRATAGLIDGVFVFCLPGSNGAVRDGWDKV
                     ISAQLDSRHKPCNMVELMPRLLEQ"
     gene            1333..1803
                     /gene="moaC"
                     /locus_tag="PLH31_13475"
     CDS             1333..1803
                     /gene="moaC"
                     /locus_tag="PLH31_13475"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQR90241.1"
                     /translation="MSKLTHIDDQGRARMVDVSDKTSTTREAVAVGFVRMSAETLALA
                     VSGTGRKGDVRAVAEIAGVMAAKKTSDLIPLCHPLALSKVVVEVEPGEGGLAVTARVK
                     TTGPTGVEMEALTAVSVACLTIYDMLKAAEKGMVIEAVRLVEKTGGKSGDWTAG"
BASE COUNT          300 a          647 c          711 g          292 t
ORIGIN      
        1 ggcgcccggc ctgccgtagc gcgccatatg tcgacgaccg gtggttaagg ggcgcggcta
       61 gagatggcga gggttctgct gtttgggcgt ctggcggatc aggcgggctg gcgcgagcgg
      121 gccttcgagg cgacgtcgct gacggcgctg cgcggtctga tcgcgactga cgatcccgag
      181 ctcggcgatg ccctgggcgg cccgggcgtt caggtcgcgg tcgacaagac gctggtgcgg
      241 ggcgaggcgg ccctgaccgc cgccaccgag gtcgcctttc tgccgccgat gagcggcgga
      301 tgacgacggg ctcaaagatc atcttgaccg ccgagccgtt cgagccgggc gcgctgctga
      361 cgcggttctg cgctggccgc gcggagaccg gcgcggtcgc cagcttcacc ggcctcgccc
      421 gggccgagca gggccaggcg gcggcgctgg agctggaggc ctatcccggc ttcaccgaca
      481 cggcgatcag cgacttcgcc gaggccgcca ggacacgctt ccatctgcac gactatcttg
      541 tcgtgcaccg caccggccgc attgcgccgg gcgaggccat cgtcttcgtg gccaccgccg
      601 ccggtcaccg ccgcgaggcc ttcgaggcct gcgacttcct gatggactgg ctgaaaagcc
      661 gcgcgccgtt ctggaagaaa gagcatggcc cggacggcgc ccgctggatc gagcccacgg
      721 accgcgatag gaccgacgcc gagcgttggg atcaggagta gatcatgtcg gcagcaggcc
      781 tcacccccgg cggcgggatc aagccggacc ttcccttcac gccggtgcgc gtggcgatcc
      841 tgaccatttc cgacacccgc gacgaggaaa gcgacacctc cggccaggtg ctggtcgatc
      901 gcgtcaaggc cgcgggtcac gagctgggcg gccgggcggt ggtgcgcgac gacatcccgc
      961 agatccgcga ccaggtgcgt aactggattg ccggcggcca ggtcgacgcc atcgtcacca
     1021 ccggcggcac cggcctgacc ggccgcgacg tgacggtcga ggcgctggag ccactgttca
     1081 acaagaagat cgacggcttt tcggtggtct tccacatggt cagctacgcc tcggtgggtc
     1141 tctccaccct gcagtcgcgg gccaccgctg gcctgatcga tggcgtcttc gtgttctgcc
     1201 tgccgggcag caacggcgcg gtgcgcgacg gctgggacaa ggtcatttcc gcccagctcg
     1261 acagccgcca caagccctgc aacatggtcg agctgatgcc ccgactactc gagcaatagg
     1321 gctgctggag ccgtgagcaa gctcacccat atcgacgacc agggccgggc tcgcatggtc
     1381 gatgtgtcag acaagacctc gacgacgcgc gaggcggtgg cggtcggctt cgtgcggatg
     1441 agcgccgaaa ccctggcgct cgccgtctcc ggaaccggtc gcaagggcga tgtccgcgcg
     1501 gtggccgaga tcgccggcgt catggccgcc aagaaaacct ccgacctgat ccccctgtgc
     1561 cacccgctgg ccctgagcaa ggtggtggtc gaggtcgagc cgggcgaggg cggcctcgcc
     1621 gtcaccgcgc gggtcaagac caccggcccg accggcgtcg agatggaggc cctgaccgcc
     1681 gtctcggtgg cctgcctgac gatctacgac atgctgaaag ccgccgagaa gggcatggtc
     1741 atcgaggccg tacgcctggt cgagaagacc ggcggcaagt cgggggactg gacggcgggt
     1801 tagccttgga cgcgagttct cgacttcacg cggcgggccc gctgaaggtg gggaggcggc
     1861 ggagcgtccg ggccaggccc ggatgagtgt gtagaggagt accgtttcgg ccgtggccgg
     1921 agcgctccgc gcggttgttg gtcaggagca
//
LOCUS       DAOMEO010000645         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6112_length_1950_cov_2.282322, whole genome shotgun sequence.
ACCESSION   DAOMEO010000645 DAOMEO010000000
VERSION     DAOMEO010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6112_length_1950_cov_2.282322"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="PLH31_13480"
     CDS             complement(<1..114)
                     /locus_tag="PLH31_13480"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HQR90242.1"
                     /translation="MTQAHQGRVLVIAGSDSGGGEGIQADIMTITALGGYAA"
     gene            complement(1055..1723)
                     /locus_tag="PLH31_13485"
     CDS             complement(1055..1723)
                     /locus_tag="PLH31_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HQR90243.1"
                     /translation="MSKGPHIANETASSWADAAEQRVLDEAVRLAPAAGWNARLTERA
                     AQAAGLSPGETQLLLPEGARDLAALLSRRHDAAAMDRLAIHDPAAMKIRDKIRAGVVA
                     RLDAVAADAEVMRALAAFLAFPTNLALALRLTWESSDVLWRWAGDTATDENHYSKRAI
                     LSGILVSTLAVDMASGRDSALAHLDARIDNVMAFEKWKAGIKPMDLASELVTALAKMR
                     YGRG"
BASE COUNT          346 a          624 c          627 g          353 t
ORIGIN      
        1 ggccgcatag ccgcccaggg cggtaatggt catgatatcg gcctggatcc cctcgccgcc
       61 gccggagtcc gatccggcga tgacaagaac gcggccttgg tgagcttggg tcatggcgca
      121 agtcctaggg gggaatcggg gaggtgcgaa gggggcggtt tgcggcaacc acagggacgc
      181 cccctccgtc atgtcgcgtc tccgcgccgc cgtagtggat ccccccaggg ggagttatat
      241 atttgatgaa gcagacttcg gaatccgtcg ttcgatcaac gaacaaggcc cgatccaatg
      301 aaccttacct ccgcaatctt tcaagacgcc gataatgcca gcgagcatct ggaaaatacg
      361 cgttggccgc atgatccgat ctaccctcac tgcggcgtta tgaatcaagc gaccgagctt
      421 aacagcaagt ccacccgcgc gggccatatc agtacagccc ggggtgcgag caattcacgg
      481 tgacggtcgg aacgtgtttg agcgccccaa ggggccgctc aacaggtggc tgctggagac
      541 cttccccttg tcgtctagca agaagggcat ttcggctcag cagatcggtc gtagccttgg
      601 cgtgatctac aaaacggctt ggttaatgtg ctaccgcatc cgcgaggcga tgagcccttc
      661 agagcccggc ccactgggtg gctaaggcag ggtgattgaa gccacttcac cagttcaacg
      721 ccgtctatgc aattctcgtg cttatcctaa cgcaaagtcg ggcagaaaca cttgagcaag
      781 cactcgttga attaggaacg gatttgaaac ttccgccgcc gcttcgggtc cgaccgcagc
      841 gcggagcgcg cccaccaaag ccgccagaat agcgtcgtcc tccagcttcg cggctagcac
      901 gcgctggaag acgacgtcat cagaagcgcg ggcccgtcgg cttgggtcat tagccccata
      961 ggtttctttg ttatgtacat ccccacagag ggagaggatc caataaggcg gcgcggagac
     1021 gcaacgtgac ggaggggcgt ctggcggttg ccggctaacc ccgcccgtac cgcatcttcg
     1081 ccagcgccgt gaccagctcg ctggccaggt ccatcggctt gatcccggcc ttccacttct
     1141 cgaaggccat gacgttgtcg atgcgggcgt ccagatgggc cagggccgaa tcccggcccg
     1201 aggccatgtc gacggccagg gtcgagacca ggatgccgga gagaatcgcc cgcttggaat
     1261 agtggttctc gtcggtggcc gtatcgcccg cccagcgcca caggacgtcg ctgctttccc
     1321 aggtcaggcg cagggccagg gccaggttgg tggggaaggc caggaaggcg gcgagcgccc
     1381 tcatgacctc ggcgtcggcg gccacggcgt ccagccgggc cacgacgccg gcgcggatct
     1441 tgtcgcggat cttcatcgcg gcggggtcgt ggatcgccag cctgtccatc gccgccgcgt
     1501 catggcgtcg cgacagcagg gccgccaggt ccctggcgcc ttcgggcagc agcaactggg
     1561 tttcgccggg cgacaggcca gcggcctggg cggcgcgctc ggtcaggcgg gcgttccagc
     1621 cggcggccgg cgccaggcgc acggcctcgt ccagcaccct ctgctcggcc gcgtcggccc
     1681 agctgcttgc ggtctcgttt gcgatgtggg ggcctttgct catgaatccg agtctacacc
     1741 gtggtttagg ccgcgtcgcg catggacagg gcccattcac tctgctatca gccgcccctc
     1801 gtttcccgga agcccccctt ccgggtttat caggttttgt tcgcccgccc ggcccctgag
     1861 agggactcgg gacattggtc gggccgtcga atggagagag acccctggtc cagattttcg
     1921 tccgcgacaa caacgtcgat caggccctca
//
LOCUS       DAOMEO010000646         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6114_length_1950_cov_2.179420, whole genome shotgun sequence.
ACCESSION   DAOMEO010000646 DAOMEO010000000
VERSION     DAOMEO010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_1950_cov_2.179420"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLH31_13490"
     CDS             <1..230
                     /locus_tag="PLH31_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90244.1"
                     /translation="PYRNWPAASRGPILLFALDCAANKWIDLGMDFTGDLGIGKGWRN
                     GEKIEDISAAVGGAGKLACEARDSLPKADLP"
     gene            391..1185
                     /locus_tag="PLH31_13495"
     CDS             391..1185
                     /locus_tag="PLH31_13495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR90245.1"
                     /translation="MATPSALLNVMIDAARKAARGLARDFGEVTELQVSKKGAADFVT
                     NADLKAEQTIFELLEKARPGYSFLGEERGLIEGTDKTHTWIVDPLDGTTNFMHAIPHF
                     AVNIALQREGEVVAGVTYNPITHDLFWVEKGKGAFLGAEKRLRVAARRHLDEAVLATG
                     VPFLGKPGHAQFLKELHQVSQKVAGVRRFGAAALDLAWVAAGRFDGYWERNLGAWDVS
                     AGVLMVTEAGGKVSTIEEHGNPVEGASIVAANQDLHPLLLEKLRLA"
     gene            complement(1235..1594)
                     /locus_tag="PLH31_13500"
     CDS             complement(1235..1594)
                     /locus_tag="PLH31_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90246.1"
                     /translation="MKLIAVQSAYEFARLFVTSLVATQTGALVALIALGAQKAPPNAF
                     TQNYSLAVFLIGLSLALGLLTAMLAYFNQYLLAFREEQTWLLWTAWVTGAASLLTLVL
                     GGLLAYWAIIERTGFPK"
     gene            1764..>1950
                     /locus_tag="PLH31_13505"
     CDS             1764..>1950
                     /locus_tag="PLH31_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQR90247.1"
                     /translation="MTLLTRRALTTAALAGTFLPGLARAQGKTPLVIAHRGASGYRPE
                     HTALAYELAIAQGCDFIE"
BASE COUNT          366 a          661 c          600 g          323 t
ORIGIN      
        1 gcccctaccg gaactggccc gccgccagtc ggggtccgat cctgctgttc gcgctggact
       61 gcgccgccaa caaatggatc gacctgggca tggacttcac cggcgacctg gggatcggca
      121 agggctggcg caacggcgag aagatcgagg acatcagcgc agcggtcggc ggcgcgggca
      181 agctggcctg cgaggcccgg gacagtctgc ccaaggcgga cctgccctag cgatcagagg
      241 aagaaaaccc ctcatcttgc tttcggcggc cctgaagact aggttccgcg cccgatcttt
      301 ctgaccgccg ctcgcatatc ccccgccgtc ccgccctttc acaacagaga gggggacgct
      361 gcgcaggggg acctatgagg aaatcccgcc ttggccaccc cctccgccct tctgaacgtg
      421 atgatcgacg ctgcccgtaa ggcggcgcgt ggcctggctc gcgactttgg cgaagtcacc
      481 gagttgcagg tttccaagaa gggcgcggcc gacttcgtca ccaatgccga cctcaaggcc
      541 gagcagacca tcttcgaact gctggaaaag gcccgccccg gctacagctt cctgggcgag
      601 gaacgcggcc tgatcgaggg caccgacaag acccacacct ggatcgtcga cccgctggac
      661 ggcaccacca acttcatgca cgccatcccg cacttcgcgg tcaatatcgc cctgcagcgc
      721 gagggcgagg tggtggccgg cgtcacctac aaccccatca cccacgacct gttctgggtc
      781 gaaaagggca agggcgcctt cctgggcgcc gaaaaacgcc tgcgcgtcgc cgcccgtcgc
      841 catctggacg aagccgtgct ggccaccggc gtgccgttcc tgggcaagcc aggccacgcc
      901 cagttcctga aggaactgca tcaggtcagc cagaaggtcg ccggcgtccg ccgcttcggc
      961 gccgccgccc tggacctggc ctgggtcgcg gccggccgct tcgacggcta ctgggagcgt
     1021 aatctgggcg cctgggacgt gtcggccggc gtgctgatgg tcaccgaggc cggcggcaag
     1081 gtctcgacca tcgaggagca cggcaacccg gtcgagggcg cctcgatcgt ggccgccaac
     1141 caggacctgc atccgctgct gctggagaag ctgcggctgg cctgatgctg atggcccgag
     1201 cgctgtgtca atcagcgctc ggggctgctc aatatcactt aggaaagcct gtacgctcga
     1261 taatggccca gtaggccagc aaccctccaa ggacgagcgt tagcaagctg gcggcgcctg
     1321 tgacccatgc tgtccatagc agccacgtct gttcttcgcg gaaggcgagg aggtattggt
     1381 tgaagtaggc caacatcgca gtcagcagtc caagggcaag cgacagcccg atcaaaaaga
     1441 ccgccaagct atagttttgc gtaaaggcgt tgggaggggc tttctgagca cctagcgcaa
     1501 ttagagcgac gagcgctcca gtttgagtgg ctaccaacga ggttacaaac agccgagcga
     1561 actcgtatgc cgattggacg gcaatgagtt tcatctgacg gtcgtgagct tcttgcgaat
     1621 cttggtttgt catttcatcc ccgcgccttc tgaaaactct accacatatc agatggggct
     1681 cgctctcatc ggcgatgcga gccgacagcc cgacacgcgc agtctctcgc caaaatgtca
     1741 caagtcagcg ctaaatcgcc gccatgacct tgctgacccg tcgcgccctg accaccgccg
     1801 ccctcgccgg caccttcctg ccgggcctgg cccgcgccca gggcaagacg cccctcgtca
     1861 tcgcccaccg gggcgcttcg ggctatcgcc ccgaacacac ggccctggcc tatgagctgg
     1921 ccatcgcgca gggctgcgac ttcatcgagc
//
LOCUS       DAOMEO010000647         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6152_length_1941_cov_2.550901, whole genome shotgun sequence.
ACCESSION   DAOMEO010000647 DAOMEO010000000
VERSION     DAOMEO010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_1941_cov_2.550901"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1164)
                     /locus_tag="PLH31_13510"
                     /pseudo
     CDS             complement(55..1164)
                     /locus_tag="PLH31_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1558..>1941)
                     /locus_tag="PLH31_13515"
     CDS             complement(1558..>1941)
                     /locus_tag="PLH31_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90248.1"
                     /translation="VEPWTLAGRASWASKFQGAGVVLSRPLAIALNARIDRLIAVGLV
                     GQDWKTPFGAAPSAPRTPALAQVSALCAAGDAPAWIVAPIAPGAAPDLTLKNRSWTAR
                     QPYELDLAGRWFVTRTYAVIPCVGR"
BASE COUNT          375 a          599 c          646 g          321 t
ORIGIN      
        1 cccggtttaa ggttggtgtt taagcgggca ttatgttcga gcgcttcttg gtcggaagat
       61 ggtcgggaac atgatcgagt tgaacaggcc gatcagcagg gccgcgaagg ccgggatcag
      121 accgctggtc agcggcgcct gggccaggaa aaggttcagc acgccggtgt gggcggtcgg
      181 gatcatgtcc ttggtaaaga tcacgaccag acacagggcg atggcgccca cagcgaccag
      241 acccagcagg acatagtcct tgaccgcctt cagcagcgcc gagccggcga aacggccgat
      301 catcgcgaac accatgtaga aggtggtcag cttgccggtc tgctcggccg agatgttcag
      361 aatgttggtc tgctccagga acaacagcag gttcagcgag atcgccactt cggcgccgac
      421 atagaggaag atgccgatcg cgcccagatt ggcccatttc gaggtcaggg ccgtgaacgg
      481 gctgaccagc ttgccggtct tgggcgcggc gtcggtgatg cgctgacgca ccatgaagat
      541 cattaggatg aacagggcca ggcccaggcc gatggccaga tagacgttgc tgacaaaacc
      601 cagagcctgc tcgcgcatgg cctgggtgac gacgatgtcc ttctcgaaga tctcgccctt
      661 cagcaggaag ttcgcgccga accacaggcc gcaggcggcg cccagcgagt tgaaggcctg
      721 ggacaggttc agtcggaacg aggagtcctc gggcttgccc agcgaggcga tcagcgggtt
      781 tgcggccacc tgcagcaggg tgatgcccga ggccatcacg aagagcgcgg tgagcacgag
      841 gctgtaggtg tgcagcttgg cggctgcgat ggcgatgaag caaccggcga cgatgccgcc
      901 caggccaacc atcaccgatc tggcgtagcc gaacttggac aggaaggccg ccgagggcag
      961 cgacatcagt ccgtaggcga tgaagaaggc aaagcccgtc aggttggctt cgacggaggt
     1021 cagcgtgaag accgtcttca tggtcttcag cagcgggtcc agcaggttgg tgaccagcgc
     1081 ccagatgaag aacaggcagg tgatatagac gacggccagg gccacgccct tgcccttttg
     1141 ggcggcggtt gctgcattag ccattggtca gatctcgccc tgcggagacc ggccgagcgc
     1201 cgatccgttc accgtggcgc acgtccgcgc accgcaaccc aagcttgtca tgattcactc
     1261 ccctgtccgt cttcgcggga cgcggcattg cgtccagacg tgacaacgtt gtcaagagag
     1321 ggtgcggcgc agcatggatt ttcgcgccca gtttgccgga acaaaaggcc cctcactgcg
     1381 gttattcgcc tagctgggga gagctttcat gaccacgctc gtcatcgtca tcacgtcagg
     1441 cattttcgcg ctcgcggcca ttattggcct gatcgacctg ttcgttcact tggtctgccg
     1501 gagcgcccgg tcgctccgca cgccagacgc ccttcaggag cgtgggagcg agccccgtca
     1561 acgtcccacg cacggtatga ccgcataggt ccgggtcacg aaccaacgcc cggccagatc
     1621 cagctcatag ggctggcgcg cggtccagga acggttcttg agagtgagat caggggcggc
     1681 gccaggcgcg atcggcgcga cgatccaggc cggcgcatcg cctgccgcac acagagctga
     1741 cacctgcgcc agggccggcg tcctgggcgc cgatggcgcg gcgccgaagg gcgtcttcca
     1801 gtcctgcccc accagtccga cggcgatcag ccggtcgatt cgggcgttca gggcgatcgc
     1861 cagcggccgc gacagcacca cgcccgcccc ctgaaacttg ctggcccagc tggcgcgacc
     1921 cgcaagggtc cagggctcga c
//
LOCUS       DAOMEO010000648         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6157_length_1941_cov_1.977731, whole genome shotgun sequence.
ACCESSION   DAOMEO010000648 DAOMEO010000000
VERSION     DAOMEO010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6157_length_1941_cov_1.977731"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1462
                     /locus_tag="PLH31_13520"
     CDS             5..1462
                     /locus_tag="PLH31_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR90249.1"
                     /translation="MEAQIAAMERSQAVIEFKLDGTILRANANFLGAMGYTAGEVEGR
                     HHSMFVPAGEAETAEYRDFWRELNRGQFVARKFLRIGKGGQACWIQASYNPVLDETGK
                     PYKVIKFATDVTAVENERAQAEAERQQVAEEQSTVVAHLADSLKRMAQGDLTARITAP
                     FNGRYQQIKDDFNAAIDSLREAMSAIAASTSGIRGGSDEIASASDDLSRRTEQQAASL
                     EETAAALDQITATVKRSADGAKQASAAASGARVDAARSGEVMRDAVSAMGEIEQSSGQ
                     ITNIIGVIDEIAFQTNLLALNAGVEAARAGDAGRGFAVVAQEVRALAQRSADAAKEIK
                     GLIAASTAQVDRGVRLVGDTGQALAGIVTKITEIDVLISEIAQSSQEQATGLNQVNAA
                     VNQMDQVTQQNAAMVEEATAAASNLRTEAGELARLVAGFEVGGQDQRSAGPVLAQAGR
                     HAPLRNPVAQAQARIASYARPGSAQTATAREWEEF"
BASE COUNT          344 a          677 c          638 g          282 t
ORIGIN      
        1 gagcctggaa gctcagatcg ccgccatgga gcggtcgcaa gcggtgatcg agttcaagct
       61 ggacggtacg atcctgcggg ccaatgccaa ctttcttggc gccatgggtt acacggccgg
      121 cgaggtcgag ggccgccacc acagcatgtt cgttccggcc ggcgaggctg agacagccga
      181 gtatcgcgac ttctggcgcg agctcaatcg cggccagttc gtcgcccgca agttcctgcg
      241 catcggcaag ggcggccagg cctgctggat ccaggcgtcc tacaaccccg tgctggacga
      301 gacgggtaag ccgtacaagg tcatcaagtt cgccaccgat gtcaccgcgg tcgagaacga
      361 gcgcgctcag gccgaagccg agcgccagca ggtcgccgag gagcagagca cagtcgtcgc
      421 ccatctggcc gacagcctca agcggatggc ccagggcgac ctgacagcgc ggatcaccgc
      481 gccgttcaac ggccgctacc agcagatcaa ggacgacttc aacgcggcca tcgacagcct
      541 gcgtgaggcg atgagcgcca tcgccgcgtc caccagcggc attcgcggcg gctccgacga
      601 gatcgccagc gcctccgatg atttgtcgcg tcgcaccgag cagcaggccg ccagcctgga
      661 agagaccgcg gcggcgctcg accagatcac cgcgacggtc aagcgcagcg ccgacggcgc
      721 caagcaggct tccgccgccg cctccggcgc ccgggtcgac gccgcccgat cgggcgaggt
      781 gatgcgcgac gccgtcagcg ccatgggcga aatcgagcag agctcgggcc agatcaccaa
      841 catcatcggc gtgatcgacg agatcgcttt ccagaccaat ctgctggccc tcaacgccgg
      901 cgtcgaggcc gcccgggctg gcgacgcggg ccggggcttt gcggtggtgg cccaggaagt
      961 gcgggcgctt gcccagcgct cggccgacgc cgccaaggag atcaagggcc tgatcgccgc
     1021 cagcaccgcc caggtggacc ggggcgttcg cctggtcggc gacacgggtc aggccctggc
     1081 cggcatcgtc accaagatca ccgagatcga cgtgctgatt tccgagatcg cccagtcctc
     1141 ccaggagcag gccacgggcc tcaaccaggt caacgccgcc gtcaaccaga tggatcaggt
     1201 cacccagcag aacgccgcca tggtcgagga ggcgacggcg gcggcctcga accttcggac
     1261 cgaagcgggc gaactggcgc gcctggtcgc cggtttcgag gtcggcggac aagaccagcg
     1321 gtcggcgggt ccggtcctgg cccaggccgg ccggcatgcg cccctgcgca atccggtcgc
     1381 ccaggcccag gccaggatcg cctcctacgc tcggcccggt tcggcccaga ccgccaccgc
     1441 ccgggaatgg gaagaattct gacgatgtga cgcggtcgcg gcccctatcg ccggggggcg
     1501 taacgcccta tattcgcaag cggcgggtct gctgtggccc tcgtcgaaga tttctattcc
     1561 cagggacctt aatctctcta tcgggacctg ttccctcccg tggccgtggc tgcggggaca
     1621 cggggcccac ctgtaccaaa caggttcgag tggattgaaa cgtcttcacg gttcttcatg
     1681 gcgccgccac ctcattccta aaaactgtca tcccgccccg gtcttgagcc gggggcagag
     1741 cgcgcagacc cgggatcacc gcaagcgccg cgtctgccgc gttcccggct cggcgcgtcc
     1801 gttggacgct gggccgggat gacagtattt aacgccatgg ttcacgccga tcccgcctcc
     1861 gccaagaccg ccctgcgggt cttcgtgcgc aaccagcgca agcagctgct taaaatgggc
     1921 ctcgacatcc gcgaatacaa a
//
LOCUS       DAOMEO010000649         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6159_length_1940_cov_2.462599, whole genome shotgun sequence.
ACCESSION   DAOMEO010000649 DAOMEO010000000
VERSION     DAOMEO010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_1940_cov_2.462599"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>265
                     /locus_tag="PLH31_13525"
     CDS             <1..>265
                     /locus_tag="PLH31_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR90250.1"
                     /translation="RWREKPGAGVLLDLGPHLIDQALVLFGRPLAVYADVAVQKDGGV
                     AGDYFHLLLRYPRLRVLLHASQMTQATNLRLAVHGTAGSFIKSG"
     assembly_gap    266..275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <276..538
                     /locus_tag="PLH31_13530"
     CDS             <276..538
                     /locus_tag="PLH31_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQR90251.1"
                     /translation="KAGLRPGAPGYGDDPRLGVLIQMEDGEPVSTPVQPLPADYRAYY
                     AGIREAITMGAPSPVPVEEALLVMEVIDAAQRSAVERREITL"
     gene            535..1002
                     /locus_tag="PLH31_13535"
     CDS             535..1002
                     /locus_tag="PLH31_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HQR90252.1"
                     /translation="MSLPPDLALIAEQERMLVFPRFTLDTAWSLGTALRDAALARAAP
                     VAIDISLPERPLFHAALPGAGPDNADWVRRKRNTVLRLGTSTLGIGLKLTASGETLEG
                     RYGLSLRDHAAHGGGFPLILEGLGCIGAITVSGLPQVEDHGLIVQTLEALLQT"
     gene            complement(999..1307)
                     /locus_tag="PLH31_13540"
     CDS             complement(999..1307)
                     /locus_tag="PLH31_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90253.1"
                     /translation="MSNAEVLFQQPVERRAESRRACLLRGDLIIGGGRAEPVAVLDLT
                     AFGARVAMRRTLLLPTRLLLRLTLVEGMVLYDAELAWRRGLDAGMALSGRREAKGLTH
                     "
     assembly_gap    1332..1341
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1385..>1940)
                     /gene="rsgA"
                     /locus_tag="PLH31_13545"
     CDS             complement(1385..>1940)
                     /gene="rsgA"
                     /locus_tag="PLH31_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQR90254.1"
                     /translation="IAVSARQGVGLDAVRDHIAPGETCVLIGSSGVGKSTLVNVLSGE
                     ERMATQEIRAADARGRHTTSHRQLFLLPGGGLILDTPGIREVGLIDAEEGLSVVFDDI
                     ERLAHDCRFRDCGHANEPGCAVRAALQSGALDPGRWASFQKLGLELAAVENKADRIAK
                     EAERRRQLSLQKAYRTTKKDLRGE"
BASE COUNT          312 a          686 c          619 g          303 t
ORIGIN      
        1 ccgctggcgg gaaaagccgg gggccggggt gttgctggac ctgggtccgc acctgatcga
       61 ccaggccctg gtgctgtttg ggaggccgct ggcggtctat gccgacgtcg cggtccagaa
      121 ggatggcggc gtggccggcg actatttcca cctgctgctg cgctatccgc gccttcgggt
      181 gctgctgcac gccagtcaga tgacccaggc caccaatctg cgcctggccg tgcacggaac
      241 cgccggcagc tttatcaaga gcggcnnnnn nnnnntgaag gcggggctaa ggcccggcgc
      301 gcccggctat ggcgacgatc cccgcctggg cgtcctgatc cagatggagg acggcgaacc
      361 ggtcagcacg cccgtgcagc ccctgcccgc cgactatcgc gcctattatg ccggcatccg
      421 cgaggcgatc acgatgggcg cgcccagccc cgtgccggtg gaagaagccc tgctggtcat
      481 ggaagtgatc gacgccgccc agcgcagcgc cgtggagcgt cgcgagatca ccctgtgagc
      541 ctgccccccg acctcgccct gatcgccgag caggagcgta tgctggtctt cccgcgcttc
      601 acgctcgaca ccgcctggag cctgggaacc gccttgcgcg acgccgccct ggcccgcgcg
      661 gcgcccgtgg ccatcgacat cagcctgccc gagcgcccgc tgtttcacgc cgccctgccc
      721 ggtgcgggcc cggacaacgc tgactgggtc cgtcgcaagc gcaacacggt tctgagactg
      781 ggaaccagca ccctgggcat aggcctgaaa ctgaccgctt cgggcgagac gctggaaggt
      841 cgctatggcc tgtcgctgcg cgaccacgcg gcccatgggg gcgggtttcc gctgatcctt
      901 gaaggcctgg gctgcatcgg cgcgatcacg gtctcgggcc tgccgcaggt ggaagaccat
      961 ggcctgatcg tccagacgct agaggcgctg cttcagacct agtgggtcag gcccttggct
     1021 tcgcgccggc ctgacaaggc catgccggcg tccaggccac gtcgccacgc cagctcggcg
     1081 tcgtagagca ccatgccctc gaccagggtc aggcgaagca gaaggcgcgt cggcagcagc
     1141 aaggtccgcc gcatcgccac ccgcgcgcca aaggccgtca ggtccagcac cgccaccggc
     1201 tcggcgcgcc cgccgccgat gatcagatcg ccgcgcaaca gacaggcccg ccgactctcg
     1261 gcgcgccgct cgacgggttg ttgaaagagg acttcagcgt tcgacatgca gccaagctat
     1321 accgccagat gnnnnnnnnn ngggcttagc cttgttggcg aggcgcgatg gcctcgcact
     1381 catttcactc gccccggaga tccttcttgg tcgttcggta ggctttctgg agcgatagct
     1441 gccgacggcg ttcagcctcc ttggcgatcc gatcagcctt gttttccacg gcagccagct
     1501 ccaggcccag cttctgaaac gacgcccagc gaccggggtc cagagcgccg ctctgcagag
     1561 cggcgcgcac cgcgcagcct ggctcattgg catgaccgca gtcacggaac cggcaatcat
     1621 gggccaggcg ctcgatatca tcgaacacca cactcaaacc ctcttcggcg tcgatcaggc
     1681 ccacttcccg aatgcctggc gtgtcgagta tcagcccgcc gccaggcaac agaaacagct
     1741 gccgatgact ggttgtgtgc cgcccccgag cgtctgccgc acggatctcc tgggtcgcca
     1801 tccgctcctc gcctgacagg acattgacga gtgttgactt gccgacgccg gacgacccga
     1861 tcagcacgca ggtctcgccc ggcgcgatat ggtcgcggac agcgtccagg ccaacaccct
     1921 gacgcgccga aaccgcgatc
//
LOCUS       DAOMEO010000650         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6175_length_1936_cov_2.904306, whole genome shotgun sequence.
ACCESSION   DAOMEO010000650 DAOMEO010000000
VERSION     DAOMEO010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6175_length_1936_cov_2.904306"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLH31_13550"
     CDS             complement(<1..193)
                     /locus_tag="PLH31_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90255.1"
                     /translation="MLRPYLLLSAALLAAGSTQAEPRGQDKAPTAAQIARVNRAARVE
                     PSVGPQAGASQVYVYAEGAL"
     gene            complement(193..879)
                     /gene="trbF"
                     /locus_tag="PLH31_13555"
     CDS             complement(193..879)
                     /gene="trbF"
                     /locus_tag="PLH31_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HQR90256.1"
                     /translation="MNPFKRSSARYGATPIPETPYQRAGQAWDERLGSARVQAANWRL
                     MALGLLGLSAALTGGLVWQSARGTITPWVVEVDRLGQAVASAPASAGYRPTDPQIVWH
                     LGRFVEQVRSISADPVVVRDNWLSAYDYTTERGGLALSDYARGADPFAKVGRTQVAVE
                     ISSVIRASPDSFRVAWIERRCEDGQLAATERWTAILTVVLQPPKTAEALRKNPLGVFI
                     HALSWSREMS"
     gene            complement(879..>1936)
                     /gene="trbL"
                     /locus_tag="PLH31_13560"
     CDS             complement(879..>1936)
                     /gene="trbL"
                     /locus_tag="PLH31_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HQR90257.1"
                     /translation="GNFQALANIVFASFSGLGLKATGGAMSAADFLQPGKLAQAGIDA
                     GRPLLENAGELARFPGFFDNFVQIVVLLAAWLVVMVAFFVLAVQLLVTIIEFKLTTLA
                     GFVLVPFGLFGRTAFLAEKVLGNVMASGIKVLVLAVIVGIGSTLFDEFVHDYAGAQPT
                     LEEVLAIVLGAVTLLGLGIFGPGIATGLVSGGPQLGAGAAVGTGLAVGGMAMVGAAGV
                     GALAGAGGALAAAGAGAAGRLAPSGRGDAGLGPVAPGGGAPPQGPAGGALGAAATGHS
                     AGSRREPAGPKPAAPDPSAKPEAATSPTDANGPPAWAKDLERRQVLTHGAATTAHAVG
                     GGDHGGGGATVSLTEDH"
BASE COUNT          319 a          703 c          670 g          244 t
ORIGIN      
        1 agagcgcgcc ttccgcatag acatagacct gggaggcgcc ggcttgcggt cccaccgacg
       61 gctcgacccg ggccgcgcgg ttgacccgcg cgatctgcgc cgccgtcggc gccttgtctt
      121 gaccgcgcgg ttcggcctgc gtggaccccg ccgccagcaa ggcggcagac agaaggaggt
      181 agggacgcaa catcagctca tctcccttga ccaggacagg gcgtggatga agacgcccag
      241 agggttcttg cgcagcgctt cggcggtctt gggcggctgc aggacgacgg tcaggatcgc
      301 ggtccagcgc tcggtggccg ccagctggcc gtcctcgcag cgtcgctcga tccaagcgac
      361 gcggaagcta tcgggcgagg cgcggatgac gctggaaatc tcgaccgcga cctgggtccg
      421 gccgaccttg gcgaagggat cggcgccgcg ggcatagtcc gagagggcca ggccgccgcg
      481 ctcggtggtg tagtcataag ccgacagcca gttgtcacgc accaccaccg gatcggccga
      541 gatcgagcgc acctgctcga cgaaacgccc caagtgccag acgatctggg ggtcggtcgg
      601 ccggtagccg gcgctggccg gcgcgctggc cacggcctgt ccaagacgat cgacctcgac
      661 cacccagggc gtgatcgtgc cgcgcgccga ttgccagacc agaccgccgg tcagggcggc
      721 gctcaggccc aagagcccca aggccatcag ccgccagttg gccgcctgta cccgagccga
      781 acccagtcgc tcgtcccagg cctgaccggc gcgctgatag ggcgtctcgg gaatgggcgt
      841 cgcgccatag cgggcgctgc tgcgcttgaa ggggttcatc agtgatcctc cgtcagggaa
      901 acggtcgccc cgccgccgcc atgatcgccg ccaccgaccg catgggccgt cgtcgccgcg
      961 ccatgggtca ggacttgtcg gcgctccagg tccttggccc aggccggcgg cccgttggca
     1021 tcggtcggcg aggtcgcggc ctcaggtttg gcgctggggt ctggcgcggc gggtttgggc
     1081 ccagcgggct cgcgcctcga tccggcggag tggcccgtcg cggcggcgcc aagcgcgccc
     1141 ccagccggtc cttggggcgg cgctccgccg ccaggcgcaa ccggtccgag acctgcatcg
     1201 ccgcgccccg agggcgccag ccgacccgcc gcgccggctc cagcggccgc cagtgccccg
     1261 cccgcgccgg ccaaggcgcc gacaccggcc gcgccgacca tggccatgcc gccgacggcc
     1321 agccccgtgc cgaccgccgc gccggcgccc aattgcgggc cgccagagac gagccccgtg
     1381 gcgataccgg gcccgaagat gcccaggccc agcaaggtca cggcgcccag gacgatggcc
     1441 agcacctcct cgagcgtcgg ctgggcgccg gcatagtcgt ggacgaattc atcgaacagc
     1501 gtcgagccga tgccgacaat gaccgccagg acgaggacct tgatccccga ggccatgaca
     1561 ttgcccagca ccttctcggc caggaacgcc gtgcggccaa agagcccgaa cggcacgagc
     1621 acgaagccgg ccaaggtggt cagcttgaat tctatgatcg tgacaagcag ttgaaccgcc
     1681 aggacaaaga aggcgaccat cacgaccaac caggccgcga gcagcacgac gatctggacg
     1741 aagttgtcga agaagccggg aaagcgcgcc agctcgcccg cgttctccag cagcggccgt
     1801 ccggcgtcga tccctgcctg ggccagcttg cccggctgca ggaaatcggc cgccgacatc
     1861 gccccgcccg tcgccttgag ccccaacccc gaaaagctgg cgaagacgat gttggccaag
     1921 gcctggaagt tgccga
//
LOCUS       DAOMEO010000651         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6176_length_1936_cov_2.709729, whole genome shotgun sequence.
ACCESSION   DAOMEO010000651 DAOMEO010000000
VERSION     DAOMEO010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_1936_cov_2.709729"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLH31_13565"
     CDS             complement(<1..469)
                     /locus_tag="PLH31_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR90258.1"
                     /translation="MTDPIPLSGAPGSPYTRKMLAVLRYRRIAYRFLPSSGQALDGLP
                     RPKVQLLPTFYLPGADGALEAVVDSSPIIRRLEAEHAGRSVIPPDRALAFIDALIEDY
                     ADEWLTKAMFHYRWSYPADIARAAAILPCWRGAAIPDAELAERGAQFSRRQIDR"
     gene            complement(481..1575)
                     /locus_tag="PLH31_13570"
     CDS             complement(481..1575)
                     /locus_tag="PLH31_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR90259.1"
                     /translation="MRTRTGVALAVLGLVAILGAGAWLARGRIAETAMERLYEKAMAR
                     APDDGLADGLHVGLCGAGGPLPDPQRAGPCTAVLAGKRLFVVDAGSGATRNLSLMNLP
                     PARIEAVFLTHFHSDHIDGLGELMLQRWAGSGSTMPVPVHGPQGVEPVVGGFMSAYRM
                     DQGYRTAHHGAAVVPPSGFGGQARAFDAAAGQPDVVLIDEPDLKVTAFPVAHDPVSPA
                     VGYAFTYKGRTVVISGDTALSPRVEAAAKGADLLVHEALAPNLVASQQRAARAAGRQR
                     LAKIFSDIPGYHAAPEQVAALAARDKVGMLIFTHITPALPIGALEGPFLGRSRQIFTG
                     RIKVGRDGDFVSLPAGSKEIVVSDRLKMFR"
     gene            1681..>1936
                     /locus_tag="PLH31_13575"
     CDS             1681..>1936
                     /locus_tag="PLH31_13575"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HQR90260.1"
                     /translation="MQVLRTPDDRFEALADWPFAPHYATITDADGTALRLHYVDEGPR
                     DAPVVLLMHGEPSWAYLYRRMIAALAAKGHRVIAPDLIGFG"
BASE COUNT          284 a          646 c          725 g          281 t
ORIGIN      
        1 ggcggtcgat ctgccttcgg ctgaactgcg cgccgcgctc ggccagttcg gcgtcgggga
       61 tggcggcccc gcgccagcag ggcaggatgg cggcggcgcg ggcgatgtcg gccggatagc
      121 tccagcgata gtggaacatc gccttggtca gccattcgtc ggcatagtcc tcgatcaggg
      181 cgtcgatgaa ggccagggcc cggtcgggcg ggatcacgga gcgaccggcg tgctcggcct
      241 ccagccggcg gatgatcggc gaggagtcga ccaccgcctc cagcgctccg tcggcgcctg
      301 gcaggtagaa ggttggcagc agttgcacct tgggccgggg caggccgtcc agcgcctggc
      361 cggagctggg caggaagcgg taggcgatgc ggcgatagcg cagcaccgcc agcatcttgc
      421 gggtgtaggg cgagccaggc gcgccgctga gggggatagg atcggtcatg ggggcgtcgg
      481 ctatcggaac atcttgagac gatcgctgac cacgatctct ttggagccag ccggcaggct
      541 gacgaagtcg ccgtcacggc cgaccttgat cctgccggtg aagatctggc gcgacctgcc
      601 caggaacgga ccctccagcg cgccgatcgg cagggccggg gtgatgtggg tgaagatcag
      661 catgcccacc ttgtcgcgcg cggccagggc cgccacctgc tcgggcgcgg cgtgatagcc
      721 ggggatgtcg ctgaagatct tggccagccg ctggcgcccc gccgccctgg ccgcccgctg
      781 ctggctggcc accagattgg gcgccagggc ctcgtggacc agcaggtcgg cgcccttggc
      841 ggcggcttcg acgcggggcg acagggcggt gtcgccgctg atcacgacgg tgcggccctt
      901 gtaggtgaag gcgtagccga cggcggggct gaccgggtca tgggccaccg ggaaggcggt
      961 gaccttcagg tcgggctcat cgatcagcac cacgtccggc tggccggcgg cggcgtcgaa
     1021 ggcgcgggcc tggccgccaa agcccgaggg cggcacgacg gctgcgccat ggtgggcggt
     1081 gcgatagccc tggtccatcc ggtaggccga catgaagccg ccgaccaccg gctccacgcc
     1141 ttgcggaccg tgcacgggca cgggcatggt cgagccgcta cctgcccatc gctgcagcat
     1201 cagctcgccc aggccgtcga tatggtcgga gtggaagtgg gtcaggaaca ccgcctcgat
     1261 ccgggccggg ggcaggttca tcagcgacag gttgcgggtc gccccggagc cggcgtcgac
     1321 cacgaacagg cgcttgcccg ccagcaccgc cgtgcagggt ccggcccgtt gcggatcggg
     1381 caggggaccg ccggcgccgc acaggcccac atgcaggccg tcggccaggc cgtcgtcggg
     1441 cgcgcgggcc atggcctttt cgtagagccg ttccatcgcg gtctcggcga tcctgccgcg
     1501 cgccagccag gcgccggcgc cgagtatcgc gacgaggccc aacaccgcga gcgcaacgcc
     1561 tgttctggtc cgcatggccg atcctcccgc ccggacgggc ttgaccccgc cacaatgtag
     1621 tgacacgcat attgtcaata tatgtcgcct gttatcgcgg cgagtcaacg ggagggtggt
     1681 atgcaggttc tgaggacgcc ggacgaccgg ttcgaggcgc tggccgactg gccgttcgcg
     1741 ccgcactacg cgacgatcac cgacgccgac ggcacggccc tgcggctgca ctatgtggac
     1801 gaagggccgc gcgacgcgcc cgtcgtgctg ctgatgcatg gcgagccgtc ctgggcctat
     1861 ctgtaccgca ggatgatcgc cgccctggca gccaagggcc atcgggtgat cgcgcccgac
     1921 ctgatcggct tcgggc
//
LOCUS       DAOMEO010000652         1935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6187_length_1935_cov_2.093617, whole genome shotgun sequence.
ACCESSION   DAOMEO010000652 DAOMEO010000000
VERSION     DAOMEO010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6187_length_1935_cov_2.093617"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLH31_13580"
     CDS             <1..238
                     /locus_tag="PLH31_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HQR90261.1"
                     /translation="ECFGDDVPYGELTSFPRAVQATDDEIVVFSWITYTDRAHRDAVM
                     AKVMADERMKPAMDNMPFDGKRMIFGGFETFLSV"
     gene            436..783
                     /locus_tag="PLH31_13585"
     CDS             436..783
                     /locus_tag="PLH31_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90262.1"
                     /translation="MIRSIAILAATAAVLTACGPKTDAEASRTEGPVVSGSEVPRNEA
                     VDTTPTTGESGPTPGASSYTEAQARSAIESAGYTGVGALTQNTNGLWQGPAMKDGKSV
                     SVSVDYKGAVTAL"
     gene            819..>1152
                     /locus_tag="PLH31_13590"
     CDS             819..>1152
                     /locus_tag="PLH31_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90263.1"
                     /translation="MSKTITRLFDDRAQALSAVEDLERAGVHHDHISLVSNNSDNWHD
                     GKAGPLGDANGDGENDVADGAGKGATTGGLIGGGAGMLAGLGMLAIPGLGPVVAAGWL
                     ASTAVGALV"
     assembly_gap    1153..1162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1163..>1461
                     /locus_tag="PLH31_13595"
     CDS             <1163..>1461
                     /locus_tag="PLH31_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90264.1"
                     /translation="GGAAGGLLGALKEAGHTDEEANVYSEGVRRGGTLVSVKTDEDRA
                     VEIQQILDGRRGVDAIRRGEAYRQSGWTQFDHNASPFSSEQVIRERDLYRSEERS"
     assembly_gap    1462..1471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1636..>1935
                     /locus_tag="PLH31_13600"
     CDS             1636..>1935
                     /locus_tag="PLH31_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HQR90265.1"
                     /translation="MTHASFSGFATADLAFLKGLAVHNDREWFTAHRAPFDEGLKPTL
                     VALVLALNEALDARDLPLAGHPKHTVFRIHRDVRFSKDKKPYKTHVSATLTRVGQK"
BASE COUNT          354 a          667 c          593 g          301 t
ORIGIN      
        1 cgagtgcttc ggcgatgacg tgccctatgg cgagctcacc tcgttccccc gcgccgtcca
       61 ggcgaccgac gacgagatcg tggtcttttc ctggatcacc tacaccgacc gcgcccaccg
      121 cgacgccgtg atggccaagg tcatggccga tgagcgcatg aagccggcca tggacaacat
      181 gcccttcgac ggcaagcgca tgatcttcgg cggtttcgag acgttcctgt ctgtctagcg
      241 cccaggcgct ccccgcaaat gttcgaaaga tctcgggtgc agtaaattca ggccaaaggc
      301 caagctttca cgtcggggca ggcgcgttct ggtcaggcta aaggtgacgt ttaagcattt
      361 cgctggctgg ccgatttccg aggaactggt catgacttga gggcttttcc ttccaccttc
      421 tgggaggaca aagtcatgat cagatctatc gccattctgg cggctaccgc cgctgtactc
      481 accgcctgcg gccccaagac cgacgctgaa gcctcgcgca ccgaaggccc cgtggtctcg
      541 ggctcggagg ttccgcgcaa tgaagcggtc gacaccacgc cgaccaccgg cgagagcggc
      601 cccaccccgg gcgccagctc ctacaccgaa gcccaggccc ggagcgccat cgagtcggcc
      661 ggctacaccg gcgtcggtgc cctcacccag aacaccaacg gcctgtggca gggcccggcg
      721 atgaaggacg gcaagtccgt cagcgtctcc gttgactaca agggcgcggt caccgcgctc
      781 tgatcccggc ctcgaacatc agcaagattg gaaaacccat gagcaagacc atcacccgcc
      841 tgttcgatga ccgcgcccag gctctgagcg ccgtcgaaga cctggaacgc gccggcgtcc
      901 atcacgacca catcagcctg gtctccaaca acagcgacaa ctggcatgac ggcaaggccg
      961 gcccgctggg cgacgccaat ggcgacggcg agaacgatgt cgccgacggc gccggcaagg
     1021 gcgcgaccac cggcggcctg atcggcggcg gcgccggcat gctggcaggc ctcggcatgc
     1081 tggccattcc tggccttggc ccggtcgtcg cggcgggctg gctggcctca accgccgtcg
     1141 gcgccctggt cgnnnnnnnn nnaggcggcg cagccggcgg cctgctgggc gcgctgaagg
     1201 aagcgggcca caccgacgaa gaggccaatg tctattccga aggcgttcgc cgcggcggca
     1261 ccctggtcag cgtcaagacg gacgaagatc gcgccgttga aatccagcag atcctggacg
     1321 gccgacgcgg cgtcgacgcc atccgacgcg gagaagccta tcgtcagtcg ggctggaccc
     1381 agttcgacca caatgccagc ccgttcagca gcgaacaggt catccgcgaa cgcgacctgt
     1441 atcgcagcga agaacgctcc tnnnnnnnnn ngaccgcgac gatggcctga tcggcgacga
     1501 caatggccgt atcgatcgcc ccggcgtgac cccctcggcg acgcgcgact actagcgttc
     1561 aggatgacgc cttcgcctca ggcgagggcg tcatccgcga ctggtctaag gctttcggcg
     1621 cgctaagctg cgcctatgac tcacgcttcg ttttccggct tcgccacagc cgatctggcc
     1681 tttctgaaag gcctcgccgt ccacaacgat cgcgagtggt tcaccgccca ccgcgcgccg
     1741 tttgacgagg gcctgaagcc cacgctcgtg gcgctggtcc tggccctgaa cgaagcgctg
     1801 gacgccaggg atctgccgct ggccggccac cccaagcaca ccgtattccg catccaccgg
     1861 gatgtgcggt tctccaagga caagaagccc tacaagaccc atgtgtcggc caccctgacg
     1921 cgtgtcggcc agaag
//
LOCUS       DAOMEO010000653         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6195_length_1933_cov_1.754526, whole genome shotgun sequence.
ACCESSION   DAOMEO010000653 DAOMEO010000000
VERSION     DAOMEO010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6195_length_1933_cov_1.754526"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLH31_13605"
     CDS             complement(<1..533)
                     /locus_tag="PLH31_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQR90266.1"
                     /translation="MARPVLSPPRPARRLIGYARVSTDEQATDAQVDELRAAGCQIIR
                     QEHGSGASRARPVLARLMREIGPSDVLVVVRLDRLARSVNHLLAVIEDLEGRQAHFRS
                     LRDPIDTSTPQGMFSLQVLGAVAQLERALIAERTKAGMKAAKARGKRAGNPGLRERRP
                     EAIRAAAAARQKVYVGDL"
     gene            complement(768..1154)
                     /locus_tag="PLH31_13610"
     CDS             complement(768..1154)
                     /locus_tag="PLH31_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR90267.1"
                     /translation="MVIDTSAIVAILRNEPEADALERLIVGAPVRLVPATCVFEARMV
                     LVSRRGEHALAEIDLWLTKIGADIIPVDAELADLATQAWLAYGKGRHPAALNFADCFS
                     YALSKRSNERLLFIGNDFSKTDILAA"
     gene            complement(1154..1411)
                     /locus_tag="PLH31_13615"
     CDS             complement(1154..1411)
                     /locus_tag="PLH31_13615"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HQR90268.1"
                     /translation="MSLNIKNPATVALADELARRQGVTKTAAIHQALSERLHRMGYGD
                     TAQARLLGELRAIRERVARLPELDTRSDEEIVGFDEHGIPN"
     gene            1619..>1933
                     /locus_tag="PLH31_13620"
     CDS             1619..>1933
                     /locus_tag="PLH31_13620"
                     /inference="COORDINATES: protein motif:HMM:NF018841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1403 family protein"
                     /protein_id="HQR90269.1"
                     /translation="MFPAFPGWARSLVTEETVEDTGFLAGAALAALHPIARHEHPIGI
                     LWRQRLALSCAAALARQNGRTEDEAALRDHWYLRREGDDPGPAGQLLKAWRALGERSA
                     MAA"
BASE COUNT          321 a          589 c          671 g          352 t
ORIGIN      
        1 aggtcgccca catagacctt ctggcgtgcc gccgcggcgg cgcggatggc ctctggtcgg
       61 cgctcgcgca ggccagggtt gccggcgcgc ttgccgcgcg ccttggccgc cttcatgccg
      121 gctttggtgc gctcggcgat cagggcgcgc tcgagctggg cgacggcgcc gagcacctgc
      181 agcgagaaca tgccttgtgg cgtcgaggtg tcgatcgggt cgcgcagcga gcggaagtgg
      241 gcctgacgtc cctccaagtc ctcgatcacc gcgagcaggt ggttgaccga gcgggcgagg
      301 cggtcgagcc gcaccaccac caggacgtca ctgggcccga tctcgcgcat cagcctggcc
      361 agcaccggcc gggcccgcga ggcgccggag ccgtgctcct ggcggatgat ctggcagccg
      421 gccgcgcgca gctcgtcgac ctgcgcatcg gtcgcctgct cgtcggtcga gacccgggca
      481 tagccgatca ggcggcgggc cggccgggga ggggagagga cggggcgggc catggggttt
      541 ccagggggcg agtcttgggg gcgattcagg cctttgtaca atcaatacag atttgagcaa
      601 acgaaccttt gcaggcgtgt tttatcgatc gcgtcagagg accgtgaagg cggccggtgg
      661 gtttcagatg gacccaaggg tcatctaacg tcaaaacgct cgcctacggc cttctgtgcg
      721 gaaaaggggc gctttcaggg accttggagg cgcgtgaggt gggatggtta ggccgcaagg
      781 atgtcggttt tggaaaaatc gttgccgata aagagtaggc gctcgttgga gcgcttcgac
      841 agggcatagg aaaagcaatc ggcgaaattc agggcggccg ggtggcgccc cttaccataa
      901 gcaagccagg cctgcgttgc caggtccgcc agctccgcgt cgaccggaat gatgtcggcc
      961 ccaattttgg tgagccacag gtcgatctcg gcgagcgcgt gctcgccgcg ccggctgacc
     1021 agaaccattc gggcctcaaa cacgcatgta gcggggacga ggcgaacagg ggcgccgacg
     1081 atcaatcgct cgagcgcatc ggcttcaggc tcattgcgca ggatcgcaac aatggcagag
     1141 gtatcgatca ccatcagttt gggattccgt gctcgtcgaa gccaacgatc tcctcgtcac
     1201 tcctggtgtc gagctcaggt aaccgcgcca cccgctcgcg gattgcacgc agctcgccga
     1261 gaagacgtgc ttgagcggta tctccatagc ccatgcggtg caggcgttcg ctcagggcct
     1321 ggtgaatggc ggcagtcttg gtgacgccct ggcggcgggc gagctcgtcg gcaagggcga
     1381 cggtcgccgg atttttgatg ttcagagaca tgggaagggt tccgaatatt ctacctttca
     1441 ggtcatttct accctgtttg gcgccctctg tcaatatgtc cgataatgca acattatcgg
     1501 tcgttcttat ctaacgacaa gcgcggcggt ttaggcgcta ttctatggca taaagcgccg
     1561 tgccgcgtta cgctgcagca tggctccgcg cgcgcgactc gttcccgatc cccttccgat
     1621 gttcccggcg tttcccggct gggcgcggtc gctggtgacc gaagaaactg ttgaagacac
     1681 tgggtttctg gccggtgcgg cgctcgcggc gctgcacccg atcgcccgcc acgagcaccc
     1741 gattggcatt ctctggcgcc agcgcctcgc gttatcctgc gcggcggcgc tggcgcgcca
     1801 gaatggccgc accgaggacg aagcggcgct gcgcgaccat tggtatctgc gccgcgaggg
     1861 agacgatccg ggaccggccg ggcagctgct caaggcctgg cgtgccttgg gcgagcgatc
     1921 ggcgatggcc gcc
//
LOCUS       DAOMEO010000654         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6200_length_1930_cov_2.275200, whole genome shotgun sequence.
ACCESSION   DAOMEO010000654 DAOMEO010000000
VERSION     DAOMEO010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_1930_cov_2.275200"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..749
                     /locus_tag="PLH31_13625"
     CDS             54..749
                     /locus_tag="PLH31_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HQR90270.1"
                     /translation="MTNVTFPAKREGLQMAPSGRSLQIVGMSDPLSPAARTSPSTARN
                     RDPILQVLRTRLPANGLVLEVAAGAGEHAVHFAAALPTLQYQPTDADPGALDSIAAWR
                     EHAGLGNLLCPLVLDASQPDIWPVERADAVLAINMIHISPWTATAGLMAGAARVLPAG
                     GGLFLYGPFIEGDTPTAPSNLEFDQSLRRRNREWGIRHLDHLVALAATHGLGLSERLT
                     MPANNLVLVFRKT"
     gene            995..1903
                     /gene="trbB"
                     /locus_tag="PLH31_13630"
     CDS             995..1903
                     /gene="trbB"
                     /locus_tag="PLH31_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HQR90271.1"
                     /translation="MLRTAMGADISDWLAEPAVVEIMLNPDGQLWLDRLGEGLSAAGV
                     AISAADAERIIRLVAHHVGEEAHAGRPRISAELPLTGERFEGLLPPLVRAPCFAIRKA
                     AVAIFGLDDYVAAEVMSAEQAQILAQAVRRRDNILIAGATSSGKTTLVNALLREIAGG
                     EDRVIILEDTRELQCAAPNQVSLRTKEAVASLRDLVRSAMRLRPDRILIGEVRGAEAL
                     DLIKAWGTGHPGGVATLHAGSAQGALLRLEQLIQEATMTVPRALIADTVDLIAVLAGR
                     GAQRRLIELTRLNGLAPDGAYQLTPA"
BASE COUNT          316 a          685 c          608 g          321 t
ORIGIN      
        1 gtcacaagcc acaaatggct acagtgatcc attgtggccc ccgtcaaaaa tgaatgacga
       61 atgtcacgtt tccggcaaaa cgcgaaggtc tgcaaatggc gccgagcggc cgaagccttc
      121 agattgtcgg catgtccgat ccgctgtctc ccgccgcgcg tacgtccccc tccacggccc
      181 gaaaccgcga tcccattctg caggtcctac ggacgcgcct gccggcgaac ggtctggtgc
      241 tggaggttgc ggcgggagcc ggcgagcacg cagttcactt cgccgcagcg ctgcccacgc
      301 tgcaatacca gccgaccgat gccgatcccg gcgctctgga tagcatagcc gcctggcgcg
      361 agcatgccgg cctaggcaac cttctgtgtc cgctggtgct ggatgcgtcg caaccggaca
      421 tctggcccgt cgaacgagcc gacgctgtcc tcgccatcaa catgatccat atctcgccgt
      481 ggacggccac ggccggcctg atggcgggcg ctgcgcgtgt gctgcctgcg ggcggcggcc
      541 tgttcctgta cggcccgttc atcgagggcg acacgccgac agcgcccagc aacctcgagt
      601 tcgatcagag cctgagacga cgcaatcgcg agtggggaat tcgccacctg gaccatctgg
      661 tcgccttggc ggctacccat ggccttggcc tttccgaacg tctcaccatg cccgcgaaca
      721 acctggtact cgtgttccga aagacgtagc cgcaaggcac gctcggccaa ggtccgtttc
      781 tccgaggcga gcgacggtcc gttcctgatg caccctgagc tagccagcac ggcctcatcg
      841 tcgtcgactt ctttctctac gccatagccc tacgcccatc atcccgctgt tgcccggccg
      901 cgaaatgacg cccctttagt cgtccccatc gtcaatgggg atcgccgtgg gccagatcca
      961 ggatcagcat cagggacaag agcgcggcgc caagatgctg cgcacggcca tgggcgccga
     1021 catctccgac tggctggccg aacccgctgt cgtcgagatc atgctcaatc ccgatggcca
     1081 actgtggctg gaccggctgg gggaggggct atcggccgcc ggcgtggcga tcagcgccgc
     1141 cgacgccgag cgtatcatcc gtctggtcgc tcatcatgtc ggcgaggagg cccatgccgg
     1201 ccggccgcgg atctcggccg agttgccgct gaccggcgag cgcttcgaag gcctgctgcc
     1261 gcccttggtt cgtgccccgt gcttcgccat ccgtaaggcg gccgtggcga tcttcggcct
     1321 cgacgactat gtcgccgccg aggtcatgag cgccgagcag gctcagatcc tggcgcaggc
     1381 ggtgcggcgt cgcgacaaca tcctgatcgc cggcgccacc tccagcggca agacgaccct
     1441 ggtcaatgct ttgctgcgcg agatcgcggg cggcgaggac cgcgtcatca tcctggaaga
     1501 cacccgcgag ctgcagtgcg cggcgcccaa tcaggtctcc ctgcgcacca aggaggcggt
     1561 ggcctcgctg cgcgatctcg tccgctcggc catgcggcta cggcccgacc ggatcctgat
     1621 cggggaggtg aggggcgccg aggcgctgga cctcatcaag gcctggggca ccggccatcc
     1681 cggcggcgtg gcgaccctgc acgccggctc ggcgcaaggg gcgctgctgc gactggagca
     1741 actgatccaa gaggccacga tgactgtgcc ccgcgccctg atcgccgaca cggtcgatct
     1801 gatcgctgtc ctcgctggtc ggggcgccca gcgccgcctg atcgaactga cccgcctcaa
     1861 cggcctcgcg cccgacggcg cctaccaact cacccccgcc tgatcccgca atctcagagg
     1921 agatcgtcat
//
LOCUS       DAOMEO010000655         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6224_length_1925_cov_2.363102, whole genome shotgun sequence.
ACCESSION   DAOMEO010000655 DAOMEO010000000
VERSION     DAOMEO010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_1925_cov_2.363102"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..467
                     /gene="cueR"
                     /locus_tag="PLH31_13635"
     CDS             54..467
                     /gene="cueR"
                     /locus_tag="PLH31_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HQR90272.1"
                     /translation="MNIGQASRASGVSAKMIRYYEEIELVRPASRTGANYREYAAREI
                     NELRFIKRGRSLGFSMAEIAQLLSLWRDRDRPSREVKAIADRHVADLDARIAGMQAMA
                     DTLRHLSQCCAGDGRPECPILADIAEASRSCDRPA"
     gene            637..1686
                     /locus_tag="PLH31_13640"
     CDS             637..1686
                     /locus_tag="PLH31_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90273.1"
                     /translation="MQAYLRKSLKPIGKTNVDLAGDIAKLLAAAPTADSPLRDAGASF
                     VPVDSDSRLALLKHEHPTPDLAAPLLPEILRDRLEQVIGERDNLEALESQGLSPTRSL
                     LFVGPPGVGKTLSARWIAARLDRPLLTLDLATVMSSYLGKTGANIRAVLDYAKSVRCV
                     LLLDEFDAIAKRRDDDGDIGELKRLVTVILQEVDDWPSGNILIAATNHGELLDPAVWR
                     RFDDVLEFGNPSPDLAFACLVDAFGPQAPDAGAWIILLSKLWCDRSFSDLTRKVHALR
                     RLAIVSNRDLGEVIGERLAADLKAGPLALRRQAAELMKAAGLSDRKVQSLSGVSRDTL
                     RRYRPSTHDSRAPGG"
     gene            1696..>1925
                     /locus_tag="PLH31_13645"
     CDS             1696..>1925
                     /locus_tag="PLH31_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90274.1"
                     /translation="MAEPTNPRYLLGGGEKLSSEIGRPPRGMGDKANPYGFAEARTCL
                     GSQWAQVGDALAALPPGALPAGQAVVVLTLHPS"
BASE COUNT          341 a          602 c          602 g          380 t
ORIGIN      
        1 gtgtcatcgg caacgccctt cgccttcgcg cggtaaagct ctgaggatcg gcaatgaata
       61 tcggccaggc ctcaagggcg agcggcgttt cggcgaagat gatccgatac tacgaggaaa
      121 tcgagctcgt acggcccgcc agccgtaccg gcgcaaacta tcgagaatac gccgcccgag
      181 agatcaacga gttgcggttt atcaagcgcg gccgaagctt ggggttctcg atggcggaga
      241 tcgcccagct actgtctctt tggagggacc gggaccggcc aagtcgggag gtcaaggcga
      301 ttgctgaccg tcacgtcgct gaccttgatg ctcggatcgc cggaatgcag gctatggcgg
      361 acactctgcg ccatttgtcc caatgctgcg ctggcgatgg ccggccggag tgtccgatcc
      421 ttgctgatat cgccgaggcg tcccggtcct gcgaccggcc cgcatgatat cagctatgac
      481 gaaatggctt cattgtcgtc gataatcgcc catggtatgg aggagctgtg atccttataa
      541 attaaggctt tttgacccac cgtggccgaa ctcgctaccc tagatacgat tttagtccac
      601 cttgctcgct tggccttggt cgggcgagcc caagatgtgc aggcctatct gcgcaaatct
      661 ctcaaaccga tcggcaagac caatgtcgat ctggcgggcg atatcgccaa actgctggcg
      721 gcggcgccaa cggccgactc gcccctgcgc gacgcgggcg cgtcgttcgt gccggttgat
      781 agcgacagcc ggctggcctt gcttaagcat gaacatccga caccggatct tgccgcgccc
      841 ctgctacccg aaatccttcg tgaccgccta gaacaagtta tcggtgaacg cgacaatctg
      901 gaggcgcttg aaagtcaggg cctctccccc actcgctcgt tgcttttcgt cggcccgccc
      961 ggcgttggaa agaccctgag cgcgcgatgg atcgccgcgc gcctggaccg gccgctgtta
     1021 acgctcgacc tggccacggt catgagcagc taccttggta agaccggcgc caacatccgc
     1081 gccgtgctcg actacgccaa gtcggtccga tgcgtgctgc tgctcgatga gttcgatgcg
     1141 atcgccaagc gtcgcgacga cgatggcgat atcggcgaac tcaagcgctt ggtgaccgtc
     1201 atcctgcagg aggtggacga ctggccatcg ggcaacattc tgatcgccgc gaccaaccat
     1261 ggtgaactgc tcgacccggc ggtctggcgc cggttcgatg atgttctaga gtttggcaat
     1321 ccgtcgcctg acttggcgtt cgcttgcctc gtagacgcct ttggccccca ggcccctgac
     1381 gccggggcct ggataattct gctctccaag ctttggtgtg accggtcctt cagtgatctc
     1441 actcggaagg ttcacgccct gcgtcggctg gcgatcgttt ccaatcgcga cctgggcgag
     1501 gtgatcgggg aaaggttggc cgcagacctg aaggccggac cgctggccct tcgtcgccag
     1561 gcggctgaac tgatgaaggc ggccggcctg tccgaccgaa aggtccagag cttgagtggc
     1621 gtttcgcgag acacgcttag gcggtaccgt ccttcgacgc acgattcgcg cgcacccggg
     1681 gggtaatcga gcgccatggc cgagccgacc aatccgcgct acctgctagg tggcggagaa
     1741 aagctgtcgt cggaaatcgg tcgccctccg cgtgggatgg gcgacaaggc caatccatat
     1801 ggcttcgccg aggcgcgcac gtgcctcgga tcgcagtggg cacaggtggg tgatgcgctc
     1861 gcagcccttc ctccgggggc gctccccgcc ggccaagcgg tcgtggtctt gacgcttcac
     1921 ccctc
//
LOCUS       DAOMEO010000656         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6225_length_1925_cov_2.335294, whole genome shotgun sequence.
ACCESSION   DAOMEO010000656 DAOMEO010000000
VERSION     DAOMEO010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6225_length_1925_cov_2.335294"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="PLH31_13650"
     CDS             complement(<1..1209)
                     /locus_tag="PLH31_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR90275.1"
                     /translation="MRQPVWVFDDTRKRKVYANKAALELWGAATLEDLQARDFGDQSP
                     AVRIRMDAISARIAQGETVHDRWTFYPHGAPVAVRTAISLIRLADGGSGMLFEAAPAE
                     VEGQEHRAIEALRHTVVLVALYDAQGTRLFANPAAMAAYPANNASFVDSFVDRAAGER
                     VWRMALAGRPAFGSFLLATSAGARWHSVDARWARDPVSGDACVLVNETDITGEIEVRS
                     ALGEARERAENAVRARQAFLANMSHELRTPLTSVIGFAGLLADSPLDDRQRLQLGRIQ
                     DAGSALMSTLNDVLDLSKLEAGGVSLQLRPFALRGMLSQAIGIVEAQASEKRLALNLS
                     VDETSPDWVLGDPERLRQVLVNLLGNAVKFTDEGGVGLTVTRRAHPDASMAQLEFAVS
                     DTGVGVPEAML"
     gene            1489..>1925
                     /locus_tag="PLH31_13655"
     CDS             1489..>1925
                     /locus_tag="PLH31_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR90276.1"
                     /translation="MASPTRAPILALKDVRLADGAKPLFDGVDLALEPRVRACLVGRN
                     GAGKSTLLKILAAKGVEADSGERSVQPGAKIIFVDQEPDITGETLLDYCTAGGAEDYE
                     AHATLADFGLDYAKSTQGLSGGERRRAALARAFAEQPDVLLLD"
BASE COUNT          334 a          670 c          629 g          292 t
ORIGIN      
        1 gagcatggcc tcgggcacgc cgaccccggt gtccgagacg gcgaactcga gctgagccat
       61 cgaggcgtca ggatgcgccc gacgggtgac cgtcagaccc acgccgccct cgtcggtaaa
      121 cttgacggcg ttgcccagca gattgaccag cacctggcgc agccgctccg ggtcgcccag
      181 cacccagtcc ggactggtct catcgacgct gaggttcagg gccaggcgct tttccgaagc
      241 ctgggcttcg acaatgccga tcgcctggga gagcatccca cgcagcgcaa acggccgcag
      301 ctgcagactg accccgccgg cctccagctt tgagaggtcc agcacatcgt tcagggtcga
      361 catcagggcc gagccggcgt cctggatccg gcccaactgg aggcgttgcc gatcatccag
      421 cgggctgtcg gccagcaagc cggcaaagcc gataacgctg gtgagcggcg tgcgaagctc
      481 gtgactcata ttggccagaa acgcctggcg ggcgcgaacg gcgttctcgg cgcgctcccg
      541 cgcctcgccc agggccgatc ggacctcgat ctcgccggtg atgtcggttt cgttgacaag
      601 cacgcaggcg tccccgctga ccgggtcacg cgcccagcgg gcgtcgacgc tgtgccagcg
      661 ggcgcccgcc gacgtggcca gaagaaacga accgaaggct ggccgaccgg ctagcgccat
      721 ccgccacacc cgctcgcccg ccgcgcggtc gacgaaggaa tcgacgaacg aggcgttgtt
      781 ggcgggatag gcggccatgg ccgcagggtt ggcgaacagc cgcgttccct gcgcgtcata
      841 gagggccacc agaacgacgg tatggcgcaa ggcctcgatg gcccgatgtt cctgcccctc
      901 cacttcggcc ggcgcggcct cgaacagcat ccccgatccg ccatccgcca ggcggatgag
      961 cgagatggcg gttctcaccg caaccggggc gccatgggga tagaaggtcc agcggtcgtg
     1021 aacggtttcg ccctgggcga tgcgcgcgct gatggcgtcc atccggatgc gcacggcagg
     1081 cgactggtcg ccaaaatcgc gggcctgaag gtcctcgagc gtcgccgcgc cccacaactc
     1141 cagcgccgcc ttgttggcgt aaaccttgcg cttgcgcgtg tcgtcgaaca cccagaccgg
     1201 ctgacgcaaa tggtcaaagc cgtccaccgc aaacgggaga cgagcagcag ccgtggatat
     1261 gtcggttttc ggcgacaagg caggcaatca tctcccctgg agggtgtctg taacaacagc
     1321 acgcttggcg gcgcgttaac gggcgattat gtcgcgccag ccgacgaccc aagccttgac
     1381 gatccaatca gacctgtgaa gccggatttt ggcgtttctt cgcggccatc cctgttatag
     1441 gccgcatcct cgcgacactc tgacgccttc cccaccgacg agcatcttat ggcttcccct
     1501 acccgcgcgc ctattctcgc cctcaaggac gtccgactgg ccgacggcgc caagccgctg
     1561 ttcgacggcg tcgacctggc gctggagccg cgggtgcgcg cctgcctggt cggccgcaac
     1621 ggggccggca agtccaccct gctgaaaatc ctggcggcca agggtgttga ggccgacagc
     1681 ggcgagcgat ccgtgcagcc gggcgcaaag atcatcttcg tcgaccagga gccggacatc
     1741 accggcgaga ccctgctgga ctactgcacc gccggcggtg ccgaggacta tgaggcccac
     1801 gcgaccctgg ccgactttgg tctggactac gccaagagta cgcaaggcct gtcgggcggc
     1861 gagcgccgcc gcgccgccct ggcccgcgcc ttcgccgaac agcccgacgt gctgctgctg
     1921 gatga
//
LOCUS       DAOMEO010000657         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6251_length_1921_cov_2.391211, whole genome shotgun sequence.
ACCESSION   DAOMEO010000657 DAOMEO010000000
VERSION     DAOMEO010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6251_length_1921_cov_2.391211"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="PLH31_13660"
     CDS             complement(<1..85)
                     /locus_tag="PLH31_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HQR90277.1"
                     /translation="MTKWAASLIRISTHEVETLQKRLADIVE"
     gene            complement(82..1419)
                     /gene="fliI"
                     /locus_tag="PLH31_13665"
     CDS             complement(82..1419)
                     /gene="fliI"
                     /locus_tag="PLH31_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HQR90278.1"
                     /translation="MRSLIAAIERIDPLTISGRVAAVNGLLIEARGGLTRLAVGARVE
                     IDRLGLPVLPAEVVGFRETRALLMPFGPVEGVAPGAEIRIMPEGATVRPTRAWLGRII
                     NAFGEPIDGLGPLPQGEVPYPLKASPPPAHARGRVGERLDLGVRSMNVFTTTCRGQRL
                     GIFAGSGVGKSVLLSMLAKEATCDAVVVGLIGERGREVREFIEETLGEEGLKRAIVVV
                     ATSDEPALTRRQAAYMTLAISEYLRDQDLEVLCLMDSVTRFAMAQREIGLAAGEPPTT
                     KGYTPTVFTELPKLLERAGPGPIRPDGSTAAPITALFTVLVDGDDHNEPIADATRGIL
                     DGHIVMERAIAERGRFPAINVLKSISRTMPGCQYDHERAIVKGARQVMAAYANMEELI
                     RIGAYRAGADPIVDRAIRLNPAIEEFLSQDKDEATSLDDSFGHLGSILESDHA"
     gene            complement(1477..>1921)
                     /locus_tag="PLH31_13670"
     CDS             complement(1477..>1921)
                     /locus_tag="PLH31_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HQR90279.1"
                     /translation="GVNPGHYECQMVKRAPVAAEAATAPWIHDPRIGAVVSAAPALGY
                     TFKAESLKALKAPVQLWRAADDEILPDPFHASAVKAALPASVDYRIVPKARHFDFLTP
                     CDDYARKNLAQLCNSAPDFDRAAFHHTFNTVVAAFFTTRLALTHP"
BASE COUNT          301 a          597 c          701 g          322 t
ORIGIN      
        1 gctcgacgat gtcggccaat cgcttctgca gggtctcgac ctcgtgggtc gagatgcgga
       61 tcagggatgc ggcccacttg gtcatgcgtg gtcactctca aggatactgc ccaggtgtcc
      121 gaaggaatca tcgaggctgg tggcctcgtc cttgtcctgg ctgaggaact cttcgatcgc
      181 cggattgagg cgaatggcgc ggtcgacgat cggatcggcc ccggcgcgat aggcgccgat
      241 gcggatcagc tcttccatgt tggcataggc cgccatcacc tggcgggcgc ctttgacgat
      301 ggcgcgctca tggtcgtact ggcagccggg catggtccgg ctgatggact tcagcacatt
      361 gatcgccggg aagcggccgc gctcggcgat ggcccgttcc atgacgatgt ggccgtccag
      421 gataccgcgg gtggcgtcgg cgataggttc gttgtggtcg tcgccgtcca ccagcacggt
      481 gaagagggcg gtaatcggcg cggccgtgct gccgtctgga cggatcgggc ccggtccggc
      541 ccgttccagc agcttgggca gttcggtgaa gacggtgggc gtatagccct tggtggtcgg
      601 cggctcgccg gcggccaggc cgatctcgcg ctgggccatg gcgaagcggg tgaccgagtc
      661 catcagacac aggacttcca ggtcctggtc acgcagatat tcgctgatcg ccagcgtcat
      721 gtaggcggcc tggcgacggg tcagggcggg ctcgtccgag gtggcgacta cgacgatggc
      781 gcgcttgagg ccttcctcgc cgagggtctc ctcgatgaac tcgcgcacct cacggccgcg
      841 ttcgccgatc aggcccacca cgacggcgtc gcaggtggct tccttggcca gcatcgacag
      901 cagcaccgac ttgccgacgc ccgagccggc gaaaatgccc agacgctggc cccggcaggt
      961 ggtggtgaag acgttcatcg atcgtacgcc caggtccagg cgctcgccaa cccggccccg
     1021 cgcatgggcg ggtgggggcg aggccttcag tgggtagggc acttcgccct ggggcagggg
     1081 ccccagtccg tcgatgggtt cgccaaaggc gttgatgatg cggcccagcc aggccctcgt
     1141 cgggcgcacg gtggcccctt cgggcatgat gcggatctcg gcgccggggg cgacgccctc
     1201 gacggggcca aagggcatca gcagagcgcg cgtttcgcgg aagccgacga cctcggccgg
     1261 caggaccggc aggcccaggc gatcgatctc gacccgggcg ccgaccgcca gacgcgtgag
     1321 gccgccgcgc gcctcgatca ggaggccatt cacggcggcg acgcgaccgg aaatggtcag
     1381 cgggtcgata cgttcaatgg cggcgatcag gctacgcaaa tcggttctcg gtggtgaagg
     1441 tccatcctcg gccgacagcg ttaaccaccg gtgaaattag ggatgtgtta gggcgagccg
     1501 ggtggtgaaa aacgccgcaa ccaccgtgtt gaaggtgtgg tggaacgccg cacggtcgaa
     1561 gtctggcgcg ctgttgcaca gctgggccag gttcttgcgg gcatagtcgt cgcagggcgt
     1621 caggaagtcg aagtgccggg cctttggaac gatgcgatag tcgacgctgg cgggcagggc
     1681 ggccttcacg gcgctggcgt ggaacggatc cggcaggatt tcatcatcgg cggcgcgcca
     1741 cagctgcacc ggcgccttca gggccttcag gctctcagcc ttgaacgtgt agcccagggc
     1801 tggcgcggcc gagaccacgg cgcctatgcg ggggtcgtga atccagggcg cggtcgcggc
     1861 ctcggcagcc accggcgcgc gcttgaccat ctggcactca tagtggccgg gattcacgcc
     1921 g
//
LOCUS       DAOMEO010000658         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6265_length_1917_cov_2.224490, whole genome shotgun sequence.
ACCESSION   DAOMEO010000658 DAOMEO010000000
VERSION     DAOMEO010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6265_length_1917_cov_2.224490"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLH31_13675"
     CDS             complement(<1..833)
                     /locus_tag="PLH31_13675"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQR90280.1"
                     /translation="MSVFVLGSINLDAVARVDDLPRPGETVAGRSLDLFPGGKGANQA
                     VAAARMGAPTRLMGAVGREDAGPGLKTKLAGYGVQVADVLELQGVPTGQAHVWVAASG
                     ENMIVVTSGANAAITPQHVTATPLEGQRVLLSQLESPAQAIEALFRAGAAKGALRILN
                     AAPALPQGAALFPLTDILILNQTELATYAKLDEEPVRLEEVSRASRKLMSRPDQVVIV
                     TLGASGVACIRRDEAFLIEGRKVKAVDTTGAGDCFCGALAACLSINMDLRDAVEMANA
                     AA"
     gene            complement(844..1644)
                     /gene="fliP"
                     /locus_tag="PLH31_13680"
     CDS             complement(844..1644)
                     /gene="fliP"
                     /locus_tag="PLH31_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516375.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HQR90281.1"
                     /translation="MMRSLLKSLIGANSQDLRRAAIISVLVALETTILPAAAFASSAI
                     NVNLGTGAGLSERVVQMVGLLTVLSLAPSIVIMTTSFVRIVVVLGLLRTAIGVQQSPP
                     NPVIISLALFLTAIVMGPTFERSYDLGVKPLLDQQMELPEAFEAAGGPVKEFMLRQVD
                     RDDLALFVRLSKIPTPKTAADTPLKVVTPAFMISELKRAFEIGFLLFIPFLVIDLVVA
                     SVLMSMGMMMLPPATISLPFKLIFFVLVDGWRLVAGSLVESFQRGAGG"
     gene            complement(1641..>1917)
                     /locus_tag="PLH31_13685"
     CDS             complement(1641..>1917)
                     /locus_tag="PLH31_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HQR90282.1"
                     /translation="GLIGLAAYALRRFGPDAFARLVTARQIRRLRVVESLALDPTRRL
                     VIFSLDGEERLMLLGEGKLLDWGPKAAAASPSPAQSLSVQRAPEPVV"
BASE COUNT          333 a          655 c          669 g          260 t
ORIGIN      
        1 gcggcggcgt tggccatctc gacggcgtcg cgcaggtcca tattgatcga caggcaggcg
       61 gccagggcgc cgcagaagca gtcgccagcc ccggtggtgt cgacggcctt gaccttgcgg
      121 ccctcgatca ggaaggcctc gtcgcgacgg atgcaggcga cgcccgaagc gccaagggtg
      181 acgatcacca cctggtcggg gcgactcatc agcttgcgcg aggcgcgcga cacctcttcc
      241 agccgcacgg gctcctcgtc cagcttggcg taggtggcca gttcggtctg gttgaggatc
      301 aggatgtcgg tcagcggaaa cagcgccgcg ccctggggca gggccggggc ggcgttgagg
      361 atgcgcaggg cgcccttggc ggcgcccgcc cggaacaggg cctcgatggc ctgggccggg
      421 gattcaagct ggctgagcag cacgcgctgt ccctccagcg gagtggccgt cacgtgctgg
      481 ggggtgatgg cggcgttggc gccgctggtc accacgatca tgttctcgcc gctggccgcc
      541 acccagacat gggcctggcc ggtgggcacg ccctgaagtt ccagaacatc ggccacctgc
      601 acgccatagc cggccagctt ggtcttcagg ccggggcccg cgtcttcacg gcccaccgcg
      661 cccatcagcc gggtcggagc gcccatgcgc gcggcggcca ccgcctggtt ggcgcccttg
      721 ccgccgggaa acaggtccag cgaccggcca gcaacggtct cgccgggacg cggcaggtca
      781 tcgacccgcg cgacggcgtc cagattgatc gaaccgagga cgaaaacgct catcgtctga
      841 accctaaccc ccggccccgc gctggaagct ctcaacaagg cttcctgcga cgagtcgcca
      901 gccatccacc agcacgaaga agatcagctt gaacggcagg ctgatggtcg ccggcggcag
      961 catcatcata cccatgctca tcagcacgct ggccaccacc aggtcgatga ccaggaacgg
     1021 gatgaacagc aggaagccga tctcgaaggc gcgcttcagc tcggaaatca tgaaggccgg
     1081 cgtcaccacc ttcagcggcg tatcggcggc ggtcttgggc gtggggatct tcgacaggcg
     1141 cacgaacagc gccaggtcgt cgcggtccac ctgccgcagc atgaactcct tgaccggccc
     1201 gccggccgcc tcgaaggcct cgggcagctc catctgctgg tccagcagcg gcttgacgcc
     1261 caggtcatag gaccgctcga aggtcgggcc catgacgatg gcggtcagga acagggccag
     1321 gctgatgatc accggattgg gcgggctctg ctgcacgccg atggcggtgc gcaacaggcc
     1381 cagcaccacc acgatccgca cgaacgaggt ggtcatgatc acgatcgacg gcgccagcga
     1441 cagcaccgtc agcaggccga ccatctggac gacgcgctcg gaaagaccgg cgccggtgcc
     1501 caggttcaca ttgatcgccg acgaggcgaa ggcggccgcc ggcaggatcg tcgtttccag
     1561 ggcgaccagc accgaaatga tggctgcgcg tcgcaggtcc tgcgaattgg cgccgatgag
     1621 ggatttgagc aggctgcgca tcagaccacg ggctccggag cgcgctggac agacaaggac
     1681 tgggcggggg agggcgaagc ggcggcggcc ttgggccccc agtccagcag cttgccctcg
     1741 cccagcagca tcaggcgctc ctcgccgtcc aggctgaaga tcaccagccg gcgggtcggg
     1801 tccagggcca ggctttccac cacgcgcagg cggcggatct ggcgcgcggt gaccagccgc
     1861 gcgaaggcgt ccggcccaaa ccggcgcagg gcgtaggcgg ccaggccgat cagcccc
//
LOCUS       DAOMEO010000659         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6277_length_1914_cov_2.230769, whole genome shotgun sequence.
ACCESSION   DAOMEO010000659 DAOMEO010000000
VERSION     DAOMEO010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_1914_cov_2.230769"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLH31_13690"
     CDS             <1..848
                     /locus_tag="PLH31_13690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR90283.1"
                     /translation="LETIIPEHTAMDGFEVQHDFPGLGRRTMLLNARNVLYETSSDTT
                     ILLAFTDITARRLIEREKEGLLERTEDLLRQKQVLLQEMQHRVANSLQIIASILMLKA
                     RAVTSEESRLHLKDAHQRVLSVAQVQSHLHASGGVDQIEVGSYLKKLCSSLAASMVGE
                     NQPIAFEVAAGDALIGSDKAVSIGLIVTELMINAIKYAFPRSQADARVTVTFESDDGG
                     WTLTVSDNGVGKIDGALPSKQGGLGTVIVQALVRQLEATMTVDVSAGMSVAIRHPRVS
                     LDEAA"
     gene            974..1558
                     /locus_tag="PLH31_13695"
     CDS             974..1558
                     /locus_tag="PLH31_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR90284.1"
                     /translation="MLTLFHAPRSRSTRFLWLLEELGATYQVKTVNIYRSVSGAGARD
                     PANPHPHGQVPALLDDGVLITESAAIALYLTDKFPAKGLGPKVGDPLRGPYLSWLAYY
                     AGVLEPAVVNLWKQRQDDEDRATYQALDNRLRSTLEESPWLLGEDFSAADMLFISLLQ
                     FARQMLPPHAVYDDWLARANARPALARALAKDDV"
     gene            1558..>1914
                     /locus_tag="PLH31_13700"
     CDS             1558..>1914
                     /locus_tag="PLH31_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HQR90285.1"
                     /translation="MRRQLPILALTGALALVSPAAALSADPATNPRVDAVLTDDAVTV
                     TEAGKTVLVYRTKSLDPTAEPGRLNYVHPLYAPDGTVLTEDRPADHLHQRGAFWSWHQ
                     VLVNGKSIGDGWFMKGL"
BASE COUNT          346 a          643 c          579 g          346 t
ORIGIN      
        1 tgctcgagac gatcattcca gaacacacgg ccatggacgg ctttgaggtt cagcacgact
       61 ttccgggcct tggccgacgg accatgctcc tgaacgcccg caacgtgctg tacgagacga
      121 gctctgacac caccattctg ctggccttca ccgacattac cgcccgccga ctgatcgaac
      181 gggaaaagga gggtctgctt gagcgcacag aggatctgtt gcgtcagaag caggtccttc
      241 tgcaggaaat gcagcaccgg gtggccaata gcctgcagat catagccagc atcctgatgc
      301 tgaaggcgcg cgccgtcacg tccgaggaga gccgcctgca cctcaaggac gcccaccagc
      361 gggtgctgtc ggtcgctcag gtgcagtcgc atcttcacgc ctctggaggc gtcgatcaga
      421 tcgaggtcgg cagctacctc aagaagctgt gctccagtct cgccgcctca atggtcggcg
      481 agaaccagcc cattgccttc gaggtcgcag ccggcgacgc gctgatcggt tcggataaag
      541 ccgtcagtat cggcttgatc gtcaccgagc tgatgatcaa cgcgatcaag tacgccttcc
      601 cccgcagcca ggccgacgca cgtgtgaccg tcaccttcga gagtgatgac gggggctgga
      661 cgctgacggt gtctgacaat ggtgtcggga aaatcgacgg cgccctgccc agcaagcagg
      721 gcggcctggg cacagtcatc gtccaggccc tggttcggca actggaagcg accatgactg
      781 tggatgtctc cgccggcatg agcgtcgcca tccgacaccc cagggtgtcg ctcgacgagg
      841 cggcttgacc gcgttcgaac gcgagccccg ccaagacacc ataagggcgg cgcagtagag
      901 gatttctgac tcgcgctggc ttgcccaagc cgtttgttcc ggttacgttc gcgttcagat
      961 agagggcccc cgcatgctca cccttttcca cgcgccgcgt tctcgctcca cccgcttcct
     1021 gtggctgctg gaagaactgg gcgcgaccta ccaggtcaag accgtcaaca tctatcgctc
     1081 agtgtccggc gcaggcgcgc gtgacccggc caatcctcat cctcacggtc aggtcccggc
     1141 cctgcttgat gacggtgtgc tgatcacgga atccgccgcc atcgccctct atctgaccga
     1201 caagttcccg gccaagggct tgggcccaaa ggtcggcgac ccactgcgag gcccctatct
     1261 cagttggctg gcctattatg cgggcgtcct tgagcccgcc gtcgtcaacc tgtggaagca
     1321 gcgccaggac gatgaggaca gggcgaccta tcaggccctc gacaaccgcc tgcgctccac
     1381 cctggaagag tcgccctggc tactgggtga agacttctct gcagccgaca tgttgtttat
     1441 cagcttgctg cagttcgccc gtcagatgct gccgcctcac gcggtctatg acgactggct
     1501 ggctcgcgcc aatgctcgcc ccgccctggc gcgggctctc gccaaggacg acgtctgatg
     1561 cgccgccaac tgccgatcct cgccctgacc ggggcgctgg cgcttgtgtc tcccgcagcg
     1621 gcgctgagcg ctgatcccgc cacaaacccc cgggtcgacg ccgttctgac ggatgatgcg
     1681 gtaacggtca ccgaagcggg aaagaccgtg ctggtctatc gaaccaagtc tcttgacccc
     1741 actgccgagc ccggccgcct gaactatgtg catccgctct acgcgccgga cggcacggtc
     1801 ctgaccgagg acaggcccgc cgaccatctg caccagcgcg gcgccttctg gagctggcac
     1861 caggtgctgg tgaacggaaa gtcgatcggc gacggctggt tcatgaaggg cctg
//
LOCUS       DAOMEO010000660         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6287_length_1911_cov_2.573276, whole genome shotgun sequence.
ACCESSION   DAOMEO010000660 DAOMEO010000000
VERSION     DAOMEO010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6287_length_1911_cov_2.573276"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLH31_13705"
     CDS             complement(<1..964)
                     /locus_tag="PLH31_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR90286.1"
                     /translation="MALDLETRDQLLDMVARFVAERLRPIEAKVAEDDAVPQDVVDEM
                     KSLGLFGLSIPEEFGGLGLNMEEECLVGVELGRTSPAFRSAFGTNVGIGSQGLVMFGT
                     DEQKAKWLPGIASGEIITSFALTEPEAGSDSASVRTLAIRDGDDYVLNGSKRYITNAG
                     KASLFTVMARTSPDTKGGSGVSAFLVPSGLPGLSVSKPEKKMGQQGAHIHDVTFDNVR
                     VPAWNRLGEEGEGFKVAMQVLDRGRLHIAAVCVGVAERLIKDCVAYAGERKQFGQAIS
                     NFQLIQAMLADSKTEALAAKALVMETARKRDAGQGVTLEAAASKL"
     gene            complement(1071..>1911)
                     /locus_tag="PLH31_13710"
     CDS             complement(1071..>1911)
                     /locus_tag="PLH31_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR90287.1"
                     /translation="VVLCIPAFDASHFTPFMPQGFAATPDAAGIKVGVMAAASLIFFA
                     FYGFDAVSTAAEETKNPKRDLTIGIVGSMAVCTLIYMVVAAVSIGASRTEVFSKSEAP
                     LVFILESLQHPRIAQLVALAAVIALPTVILAFMYGQSRIFFVMARDGLLPKSLAKVNA
                     KTGTPVLMTLLTGALSALISGWLSLKDIAELANAGTLAAFIAVGLAVIILRVQDPGRQ
                     RVFSTPIWPVVASGAILGCLYLFSSLPTQTQLYFLYAHCIGAVVYVAFGMRNSLLARA
                     ERT"
BASE COUNT          341 a          616 c          647 g          307 t
ORIGIN      
        1 acagcttgct ggccgccgct tccagggtca cgccctggcc cgcatcgcgc ttgcgcgccg
       61 tctccatgac gagggccttg gcggccaggg cttcggtctt gctgtcggcg agcatggcct
      121 ggatcagctg gaaattcgag atcgcctggc caaactgctt gcgctcgccg gcataggcca
      181 cgcagtcctt gatcagacgt tcggccacac cgacgcagac ggcggcgatg tgcaaccggc
      241 cgcgatccag cacctgcatg gccaccttga agccttcgcc ctcctcgccc agccggttcc
      301 aggctggcac ccggacattg tcgaaggtca cgtcgtggat atgggcgccc tgctggccca
      361 tcttcttctc tggcttgctg accgacaggc cgggcagacc gctgggcacc aggaacgccg
      421 agacgcccga tccgcccttg gtgtccggac tggtccgcgc catcacggtg aacagcgagg
      481 ccttgccggc attggtgatg tagcgcttgg agccgttgag gacgtagtcg tcgccgtcgc
      541 ggatcgccag ggtgcgcacc gaggcgctgt cggagccggc ctccggttcg gtgagagcaa
      601 agctagtgat gatctcgccc gaggcgatgc cgggcagcca cttggccttt tgttcgtcgg
      661 tgccgaacat caccagcccc tggctgccga tgccgacatt ggtgccgaag gcagagcgga
      721 aggccggaga ggtccggccc agctcgacgc cgactaaaca ctcctcttcc atgttcaggc
      781 caaggccgcc gaactcttcg gggatcgaca ggccaaagag cccaaggctc ttcatctcgt
      841 cgacgacgtc ctggggcacc gcgtcgtcct cggccacctt ggcctcgatg ggccgcaggc
      901 gctcggcgac gaagcgggcg accatgtcga gcagctggtc gcgggtttcg agatcgaggg
      961 ccatggcgtt ccatcctgcg tcaaacactt gttttgacgc gaactgtagc cgccaagctc
     1021 aggaatggaa gggccggcgc gtaaaacgcc ggcccaaaat caccttgccg ctaggtccgc
     1081 tcggcgcgcg ccagcaggct gttgcgcatg ccaaacgcca catagaccac cgcgccgatg
     1141 cagtgggcgt agaggaaata gagctgggtc tgggtcggca ggctggagaa cagatagagg
     1201 catcccagga tcgcgcccga agccacgacc ggccagatgg gggtcgagaa cacccgctgt
     1261 cggcccggat cctgcacccg caggatgatc accgccaggc ccacggcgat gaaggccgcc
     1321 agcgtgccgg cattggccag ctcggcgatg tccttcagcg acagccagcc cgaaatcagg
     1381 gctgacaggg cgccggtcag cagggtcatc agcaccgggg tgccggtctt ggcgttgacc
     1441 ttggccaggc tcttgggcag cagaccgtcg cgggccatga cgaagaagat ccggctctgg
     1501 ccgtacatga aggccaggat cacggtcggc agggcgatca cggcggccag ggccaccagt
     1561 tgcgcgatcc tggggtgttg caggctttcg aggatgaaga ccagcggcgc ttcactcttg
     1621 gagaacacct cggtgcgcga ggccccgatc gagacggcgg cgaccaccat gtagatcaac
     1681 gtgcagacgg ccatcgagcc gacgatgccg atggtcaggt cgcgcttggg gttcttggtc
     1741 tcctcggcgg cggtcgagac cgcatcgaag ccgtagaagg cgaagaagat caggctggcg
     1801 gcggccatga ccccgacctt gatcccggcg gcgtcgggcg tagcggcaaa gccctgcggc
     1861 atgaacggcg tgaagtggct ggcgtcgaag gccgggatgc acagcacgac g
//
LOCUS       DAOMEO010000661         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6293_length_1909_cov_2.548004, whole genome shotgun sequence.
ACCESSION   DAOMEO010000661 DAOMEO010000000
VERSION     DAOMEO010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_1909_cov_2.548004"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>785)
                     /locus_tag="PLH31_13715"
     CDS             complement(<1..>785)
                     /locus_tag="PLH31_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90288.1"
                     /translation="TALTAAQAAAAGAALVQATGGSGLITRTTEYRIDRVGVISSVSA
                     ELGNHKIELGGWFEHNSTTQWRRWYAVPVASPDLSTPYIRPGNVMAPLFTQYQGEARI
                     KQLQLHIQDTWQATERLLLQGGFKTSSQWASGRFPVQPKVGSLGGTTALPQGDIDTHN
                     WFLPAFGATYDFNGREQFYFNVQKNLRQYQAYLAGGGGPWFTGSQAAFDAFAREGKPE
                     TSWTYEVGLRTNRQFGGGVALQAQMNYYHVDFSNRLLAIPTNP"
     assembly_gap    786..795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<796..>1909)
                     /locus_tag="PLH31_13720"
     CDS             complement(<796..>1909)
                     /locus_tag="PLH31_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90289.1"
                     /translation="GDAVAGEGAAAEAPAEAGDLVDTNTIIVTGAKTTRSATAIQQAE
                     IQKILPGVSPLKAIQTLPGVLYITADPWGNNEQNAQTFVHGFAGNQLGYTMDGIPLGD
                     QSYGNYNGLSPQRAVISENVARVIVATGAGDLATPSNSNLGGTVETFSTDPLAQFGIQ
                     AAQTVGSYDASRTYLRIDSGDLGGGSSAYVSGVRQRARAWDFNGIQKGWQANAKFVRD
                     GDAGKFTAYFDYNDMVQPNEDATVFFKPSAGGTATAAQLYTPYTKPFFYPDFNGYRTG
                     YLNALGNSPAAEGSNYRNYYSDAQRTDYLGYLRYDARLADKVTWSNTAYYHHNDGAGV
                     VAGPLGQSITTAQAYLDPAYGSLPANCRFSGNNNGTK"
BASE COUNT          314 a          595 c          625 g          365 t
ORIGIN      
        1 ggggttggtc gggatggcca gcagacggtt gctgaagtcg acatgatagt agttcatctg
       61 cgcctgcagg gcgacgccgc cgccgaactg gcggttggtg cgcagaccca cctcataggt
      121 ccagctcgtc tcgggcttgc cttcgcgggc aaaggcatcg aacgccgcct ggctgccggt
      181 gaaccacggg ccacccccgc cggcgagata agcctggtac tgccgaaggt tcttctggac
      241 gttgaagtag aactgttcgc gcccgttgaa gtcatatgtc gcgccgaacg ccggcaggaa
      301 ccagttgtgc gtatcgatgt cgccttgcgg cagcgccgtg gtgccaccga gcgagccgac
      361 cttgggctgc accgggaacc tgccgctcgc ccactgcgag ctggtcttga agccgccttg
      421 caacagcagc cgctcggtcg cctgccaggt gtcctggatg tgaagttgca actgcttgat
      481 ccgggcttca ccctgatact gcgtgaacag cggggccatc acgttgccgg ggcgaatata
      541 gggcgtgctc agatcggggc ttgccaccgg cacggcatac caccggcgcc actgcgtggt
      601 gctattgtgc tcgaaccagc cacccagctc gatcttgtgg ttgccaagct ccgcgctgac
      661 cgacgagatc acgccgacac ggtcgatgcg atattcggtg gtccgggtga tcaggcccga
      721 tccaccggtc gcctgaacca gggcggcgcc tgccgccgct gcctgggccg ccgtcagcgc
      781 cgtgcnnnnn nnnnnctttg tgccattgtt gttgccgctg aaacggcagt tcgctggcag
      841 gctgccatag gccgggtcaa gataggcctg cgcagtggtg atcgactggc cgagcggccc
      901 ggcaacgaca ccggcaccat cgttgtggtg atagtacgcc gtgttcgacc aggtcacctt
      961 gtccgccagg cgtgcatcat agcgcagata gccgaggtaa tcggtgcgtt gcgcgtccga
     1021 gtagtaattg cggtagttgc tgccctcggc cgctggcgaa ttgcccagcg cattgaggta
     1081 gcccgtgcga tagccgttga agtcggggta gaagaacggc ttggtgtagg gcgtgtaaag
     1141 ctgggccgcc gtcgccgtcc caccggcaga tggcttgaag aacacggtcg cgtcctcgtt
     1201 cggctgaacc atgtcgttgt agtcgaaata ggcggtgaac ttgcctgcat cgccatcccg
     1261 gacgaacttg gcattggcct gccagccctt ctggatgccg ttgaaatccc acgcccgcgc
     1321 ccgctggcga acgcccgaga cataggccga actgccaccg ccaagatcac cgctatcgat
     1381 ccgcaggtag gtacgcgagg catcgtaact gccgaccgtc tgcgccgcct ggatgccgaa
     1441 ctgcgccagc ggatcggtcg agaaggtctc gacagtaccg ccaaggttgc tgttcgaagg
     1501 cgtggcaagg tcaccggcgc ccgtcgccac gatcacccgc gcaacgttct ccgagatcac
     1561 ggcacgctgc ggcgatagac cgttgtagtt cccatagctt tgatcgccca gcgggatgcc
     1621 gtccatggtg tagccgagct ggttgccggc aaagccatgc acgaaggtct gtgcgttctg
     1681 ctcgttgttg ccccacggat cggcggtgat gtacaggacg ccgggcagcg tctggatcgc
     1741 cttcagcggc gagacgccgg gcaggatctt ctggatttcg gcctgctgga tcgcggtggc
     1801 cgagcgcgtc gtcttggcgc cggtgacgat gatcgtgttc gtgtcgacca gatcgcctgc
     1861 ttcggcgggt gcttcggccg cggccccttc gccggcaacg gcatcacct
//
LOCUS       DAOMEO010000662         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6296_length_1909_cov_1.937433, whole genome shotgun sequence.
ACCESSION   DAOMEO010000662 DAOMEO010000000
VERSION     DAOMEO010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6296_length_1909_cov_1.937433"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1023
                     /locus_tag="PLH31_13725"
                     /pseudo
     CDS             66..1023
                     /locus_tag="PLH31_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516359.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            1096..1278
                     /locus_tag="PLH31_13730"
     CDS             1096..1278
                     /locus_tag="PLH31_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HQR90290.1"
                     /translation="MSTNRVEGAIDKGVGATKEAVGKATGNERLEAEGLAQKAKGDVQ
                     NKVGQVQDKIGDAIKR"
     gene            1404..>1909
                     /locus_tag="PLH31_13735"
     CDS             1404..>1909
                     /locus_tag="PLH31_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90291.1"
                     /translation="MTTTVPTIGARLRDWRLKRRLSQLDLACEAEISTRHLSFLETGR
                     AAPSRDMVLRLAEGLVVPLRERNALLLAAGFAPVFPERSLDDPGLEAAKIAVEALLEA
                     HMPFPTLVIDRRWTLLAHNASAALLMAGVDPGLLEGPVNLLRLSLHPDGVAPRIANLA
                     ELRGHILSR"
BASE COUNT          308 a          650 c          616 g          335 t
ORIGIN      
        1 ggcgctcgtc atcatctgac gtcggttcat gatgcttatt tccccaacca aaccgtaaac
       61 gatctctgcg gcagaacgcc aacttgcggg gcgtagcggg tctggtcaaa ctgatctggc
      121 aaagtgcgcc gccgcgcttg gagcggggtt tcgaaaaagc taggggcgag gatggtgtac
      181 gacctgtccg ttgatctgat tcaggcgacg gtgcagcttg agcagccgct caaggacggc
      241 tcgcgcacgg tgggtactgg cttcctgatc gccgatatcg acgccgatgg tcgcccccgt
      301 acgatcctga tcaccgccgc ccatgtcttt gaccgcatgc ccgcgttttc ggccaggatc
      361 ggctatcgca tccaggccaa ggacggcgcc tggcgctatg atccccagac cctgaagatc
      421 cgcgacggcg atcatccgct gtgggtcaag cacgccggcc gcgacgtcgc cgccatcgtt
      481 gtcgaggccc ccccggaatt cgccagggcc gccatccctc tggcctggct ggccaaggac
      541 gagaccttca gcgactacaa gctgggtcct ggcgacgaga tgatggccct gggcttcccg
      601 cgtggcctgt ctgccaatcc ggccggcttt ccgatccttc ggtcgggccg tgtggcctcc
      661 tatccgctgg ccccggccac cgcctttcca accttcctga tggatttctc ggtgtttccc
      721 ggaaactctg gcggcccggt gttcatggcg cagggcgcgc gccgccgacc gggctcgacc
      781 gagtcccaag aggtgcagtt catcgccggc atcctgaccc agcaggtcga gctctccggt
      841 gagcgtctcg agatcggcat cgtcacccac gccaaataca tccgcgagac catcggcctg
      901 ctcgaccagc cgcccgccct gacggtggcg gccgcggggc ggacccggat cgagcctgtc
      961 gtcgagggcg ctacggccgc ctcggcggcg gtctcggcca gcgccatggg cgtgggccgc
     1021 taggaaaaac ctcggaacct tcgcgtccgc ggacccgttc ttgtggggac tattcacccc
     1081 aacaggagca aagccatgag caccaatcgt gttgaaggcg ctatcgacaa gggcgtcggc
     1141 gccaccaagg aagccgtcgg caaggccacg ggcaacgagc gtctcgaagc cgaaggcctg
     1201 gcccagaagg ccaagggcga cgtccagaac aaggtcggtc aggtccagga caagatcggc
     1261 gacgccatca agaggtaggg tctggtcttg ataagactga ccaacggccc cgggagagat
     1321 cccggggccg ttttcgtgtc cggccccacg cgattacgtc ggaggtaatg gcatggcggc
     1381 cgggctcggc ctagcttgcc gtcatgacca ccaccgtccc caccatcggc gcgcggctgc
     1441 gcgactggcg cctcaagcgc cggctcagtc agctggacct ggcctgcgag gccgagatct
     1501 cgacccggca tctgtcgttt cttgagaccg gccgcgctgc gcccagtcgc gacatggtgc
     1561 tgcgcctggc cgagggtcta gtcgtgccgc tgcgcgaacg caacgccctg ctgctggccg
     1621 ccggctttgc cccggtgttt cccgagcgat cactggatga tccgggcctg gaagccgcca
     1681 agatcgccgt cgaggcgttg ctggaagccc acatgccgtt cccgaccctg gtcatcgacc
     1741 gccggtggac cctgctggcc cacaatgctt cggcggcgct gctgatggct ggcgttgatc
     1801 ccgggttgct ggaaggtccg gtcaatctgc tgcggctgag cctgcatccc gacggcgtgg
     1861 cccccaggat cgccaatctg gccgagctgc gcggccatat cctgtcgcg
//
LOCUS       DAOMEO010000663         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6306_length_1907_cov_2.435745, whole genome shotgun sequence.
ACCESSION   DAOMEO010000663 DAOMEO010000000
VERSION     DAOMEO010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6306_length_1907_cov_2.435745"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLH31_13740"
     CDS             <1..739
                     /locus_tag="PLH31_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HQR90292.1"
                     /translation="VTMHDFEHAKDKVMMGAERRSMAMSDAEKKLTAYHEGGHALVAL
                     RVPVADPVHKATIVPRGRALGMVMQLPEGDRYSMSYDMMTSRLAIMMAGRVAEELIFG
                     KEKITSGASSDISAATGLARNMVTRWGFSDELGTVAYGDNQDEVFLGHSVARTQNVSP
                     ETMIKIDSEVRRLVKGGEDEARRILTEELDNLHAVAKALLEFETLSGDEIIGVMKGVQ
                     PTRKDEDEKKPTLPMSSVPISPTGVTA"
     gene            831..>1439
                     /gene="folP"
                     /locus_tag="PLH31_13745"
     CDS             831..>1439
                     /gene="folP"
                     /locus_tag="PLH31_13745"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQR90293.1"
                     /translation="MTFPARPRVMGVVNVTPDSFSDGGRFLDHASALAHVRQLIADGA
                     EILDIGGESTRPGAAPVTETDELARVIPLIEAIRAHSDVAISIDTMKPAVARAAVAAG
                     ASIWNDVAALRFGPDAPEVAAELGCEVVLMHMLGEPGTMQDAPVYDDVVAQVQAFLLA
                     RADAAMAAGVARGKIWLDPGIGFGKTLQHNLALIAALPRLVAL"
     assembly_gap    1440..1449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1450..1639
                     /locus_tag="PLH31_13750"
     CDS             <1450..1639
                     /locus_tag="PLH31_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQR90294.1"
                     /translation="GASRKRFIAAIDPLGEAADARLGGSLAAHLAGAAAGVAAVRAHD
                     VRETAQALAVWQAVRAAP"
     gene            1710..>1907
                     /locus_tag="PLH31_13755"
     CDS             1710..>1907
                     /locus_tag="PLH31_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90295.1"
                     /translation="MSTVMRESEIEGLERFSFGDGGELADSLLALVLAGEKTATCWDA
                     VLGRLTEVGRLMVACDGDGRPR"
BASE COUNT          314 a          620 c          674 g          289 t
ORIGIN      
        1 ggtcaccatg cacgacttcg agcacgccaa ggacaaggtg atgatgggcg ccgagcgacg
       61 ctcgatggcc atgagcgacg ccgaaaagaa gctgaccgcc tatcacgagg gcggccacgc
      121 cctggtggcc ctgcgcgtcc ccgtcgccga tccggtccac aaggccacca tcgtgccgcg
      181 cggccgggcc ttgggcatgg tcatgcagct tcccgagggc gaccgctact cgatgagcta
      241 cgacatgatg acctcgcgcc tggcgatcat gatggccggc cgggtggccg aggaactgat
      301 cttcggcaag gagaagatca cctcgggcgc ctccagcgac atcagcgccg ctacgggcct
      361 ggcccgcaac atggtcaccc gctggggctt ctctgacgaa ctgggcaccg tggcctatgg
      421 cgacaaccag gacgaggtgt tcctgggcca ttctgtggct cgcacccaga acgtctcgcc
      481 cgagaccatg atcaagatcg acagcgaagt gcgtcgcctg gtcaagggcg gcgaggacga
      541 ggctcgccgg atcctgaccg aggagctgga caacctgcac gcggtggcca aggcccttct
      601 ggagttcgag accctgagcg gcgacgagat catcggtgtc atgaagggcg tccagccgac
      661 gcgcaaagac gaagacgaaa agaagccgac cctcccgatg tcgtcggtgc cgatctcgcc
      721 cacgggcgtt acggcctagg ggcttccgaa gactgcacgg aaaagccggt ccctcgcggg
      781 gccggctttt ttcttgcgcg gcgaccgccg ggacgcccaa aaagggtctc atgactttcc
      841 ccgccaggcc ccgcgtgatg ggcgtcgtca atgtcacccc cgacagcttt tccgatggcg
      901 gccggttcct ggaccacgcc tcggccctgg cccatgtccg ccagctgatc gccgacggcg
      961 ccgaaatcct cgacattggc ggcgagtcga cccgccccgg cgccgcgccg gttacggaaa
     1021 ccgacgagtt ggcccgggtg atcccgctga tcgaggcgat ccgcgcccac agcgacgtgg
     1081 cgatctcgat cgacaccatg aagcccgccg tggcccgcgc cgccgtcgcg gcaggggcct
     1141 cgatctggaa cgatgtcgcc gccctgcgct tcgggccgga tgcgcccgag gtcgccgccg
     1201 agctgggctg cgaggtggtg ctgatgcaca tgctgggcga gccgggcacc atgcaggacg
     1261 cgccggtcta tgatgacgtg gttgcgcagg tacaggcctt cctgctggcc cgcgccgacg
     1321 ccgccatggc ggcgggggtg gcgcgcggga agatctggct ggatcccggc atcggctttg
     1381 gcaagacgct ccagcacaac ttggccctga tcgccgccct gccgcgcctt gtcgccctgn
     1441 nnnnnnnnng ggcgccagcc gcaagcgctt catcgccgcc attgatccgc tgggagaggc
     1501 cgctgacgca cggctgggcg gctccctggc ggcgcacctg gccggcgcag cggcgggcgt
     1561 cgcggcggtg cgggcccacg acgtgcgcga gacggcccag gccctggcgg tgtggcaggc
     1621 ggtgagggcg gcgccgtgag gggaggggcg gatgctttga aggtctgtat cgggtggatt
     1681 gctgacgcac ccgcagatag cttggcctga tgagcacagt catgcgggaa agcgaaatcg
     1741 aagggctgga gcgattcagt ttcggtgacg ggggcgaact tgccgacagc ctgctggcct
     1801 tggtgctcgc cggcgagaag acggcgacat gttgggacgc tgtacttggg cgactgaccg
     1861 aagtaggcag gctaatggtc gcttgcgacg gtgacggcag gccgcga
//
LOCUS       DAOMEO010000664         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6307_length_1907_cov_2.408747, whole genome shotgun sequence.
ACCESSION   DAOMEO010000664 DAOMEO010000000
VERSION     DAOMEO010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_1907_cov_2.408747"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1050
                     /locus_tag="PLH31_13760"
     CDS             <1..>1050
                     /locus_tag="PLH31_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90296.1"
                     /translation="EDDAKPTAGFQRVIQRAVIHVQSSGREEVTGANVLVAIFSERES
                     HAAYFLQEQDMTRYDAVNFIAHGIAKKAGASEPKAVKGANSTTEEESEKPNAKTGGEA
                     LEAYCVDLNEKARQGKVDPLIGRANEVERAIQILCRRTKNNPLLVGEPGVGKTAIAEG
                     LARKIITNQVPEVLAEATIFSLDMGALLAGTRYRGDFEERVKQVVKELENHPNAVLFI
                     DEIHTVIGAGATSGGAMDASNLLKPALASGSLRCMGSTTYKEFRQHFEKDRALVRRFQ
                     KIDVNEPTVEDTIKILKGLKSYYEDFHKLKYTNDAIKVAVELSAKYITDRKLPDKAID
                     VIDEAGAGQMLLPEGR"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1061..>1907
                     /locus_tag="PLH31_13765"
     CDS             <1061..>1907
                     /locus_tag="PLH31_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90297.1"
                     /translation="DLKRAVFGQDTAIQQLSSAMKLARAGLRDADKPIGSFLFSGPTG
                     VGKTEAAKQLASTLGIEMIRFDMSEYMERHTVSRLIGAPPGYVGYDQGGQLTDAVDQH
                     PHAVVLLDEIEKAHQDVYNILLQVMDHGVLTDSNGKKVDFRNVILIMTTNAGASDAQR
                     QSIGFGRDKVVGEEEAALKRLFTPEFRNRLDAVVAFKPLTPEIIRQVVQKFVMQMEAQ
                     LADRNVTIELSDDAADWLAKNGFDELYGARPLGRVIQEHIKKPLADDILFGRLVRGGH
                     VKIILK"
BASE COUNT          394 a          630 c          593 g          280 t
ORIGIN      
        1 cgaggacgac gccaagccga ccgctgggtt ccagcgcgtg atccagcgcg ccgtgatcca
       61 cgtgcagtcg tcgggccgcg aggaagtcac cggcgccaac gtgctggtgg cgatcttctc
      121 ggaacgcgaa agccacgccg cctacttcct gcaagagcag gacatgacgc gctatgacgc
      181 ggtgaacttc atcgcccatg gcatcgccaa gaaggccggc gcgtccgagc ccaaggccgt
      241 caagggcgcc aacagcacca ccgaggaaga gtccgaaaag cccaacgcca agaccggcgg
      301 cgaggctctg gaagcctatt gcgtcgacct caacgagaag gcccgccagg gcaaggtcga
      361 ccccctgatc ggccgcgcga acgaagttga acgcgcgatc cagatcctgt gccgccgcac
      421 taagaacaac cccctgctgg tgggtgagcc gggcgtcggc aagacggcca tcgccgaagg
      481 cctggcccgc aagatcatca ccaatcaggt tcccgaggtc ctggcggagg ccaccatctt
      541 ctcgctcgac atgggcgcgc tgctggcggg cacccgctat cgcggcgact tcgaggaacg
      601 cgtcaagcag gtggtcaagg aactcgagaa ccaccccaat gcggtgctgt tcatcgacga
      661 gatccacacg gtgatcggcg ccggcgccac cagcggcgga gcgatggatg cgagcaatct
      721 tctcaagccc gcgctcgcct ccggctcgct gcgctgcatg ggttcgacca cctacaagga
      781 gttccgtcag cacttcgaga aggatcgggc cctggtccgt cgcttccaga agatcgacgt
      841 caacgagccg accgtggaag acaccatcaa gatcctcaag gggctgaaga gctactacga
      901 ggacttccac aagctgaaat acaccaacga cgccatcaag gtggccgttg agctgtcggc
      961 caagtacatc accgaccgca agctgcccga caaggcgatc gacgtgatcg acgaggcggg
     1021 cgccggccag atgctgctgc cggaaggccg nnnnnnnnnn gacctgaagc gcgcggtgtt
     1081 tggtcaggac actgcgatcc agcaactgtc ctcggccatg aagctggccc gggccggtct
     1141 gcgcgacgcc gacaagccga tcggctcgtt cctgttcagc ggccccacgg gggttggcaa
     1201 gaccgaagcc gccaagcagc tggcctcgac gctgggcatc gagatgatcc gcttcgacat
     1261 gtcggaatac atggagcgcc ataccgtgag ccgcctgatc ggcgcccctc ccggctatgt
     1321 cggctatgac cagggcggcc agctgaccga cgccgtcgac cagcacccgc acgccgtggt
     1381 gctgctcgac gaaatcgaga aggcccacca ggacgtctac aacatcctgc tgcaggtcat
     1441 ggaccatggc gtgctgaccg acagcaacgg caagaaggtc gatttcagga acgtcatcct
     1501 gatcatgacc accaatgccg gcgcttcgga cgcccagcgt cagtcgatcg gctttggtcg
     1561 cgacaaggtg gttggcgagg aagaggccgc cctcaagcgt ctgttcacgc cggaattccg
     1621 caaccgcctc gacgcggtcg tggccttcaa gccgctgacg ccggagatca tccgccaggt
     1681 cgtgcagaag ttcgtcatgc agatggaggc ccaactggcc gaccggaacg tgacgatcga
     1741 actgtccgac gacgcggccg actggctggc caagaatggc ttcgacgagc tctacggcgc
     1801 ccgccccctg ggccgggtca tccaggagca catcaagaag cccctcgccg acgacatcct
     1861 gtttggacgt ctggtgcgcg gcggccacgt caagatcatc ctcaagg
//
LOCUS       DAOMEO010000665         1907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6309_length_1907_cov_1.755400, whole genome shotgun sequence.
ACCESSION   DAOMEO010000665 DAOMEO010000000
VERSION     DAOMEO010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_1907_cov_1.755400"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLH31_13770"
     CDS             <1..383
                     /locus_tag="PLH31_13770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQR90298.1"
                     /translation="KGAERVARVLEKDDWLMKASLPGRDRAVSQILLDLSQRATKNRS
                     AAFAQLLLERVGEETPLLRRSHRDGGFIVLLAPDVPAVLLEMGFITNPEDERLLSNAS
                     SRNRVVNAVGDAIDAYFATQVRKS"
     gene            542..>1907
                     /locus_tag="PLH31_13775"
     CDS             542..>1907
                     /locus_tag="PLH31_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQR90299.1"
                     /translation="MDLKPTERWMAIAGVAVLSVIAVAGFAIAIYAAWLFHDMPDAGE
                     LQDYRPPTATRVYAWDGTLIGEYSKERRIFVPYDQIPPQLAQAFMAAEDRNFFRHSGV
                     DVGGFSRAMIKNVSNAARGRRLEGGSTITQQVAKNVLLTSDATVGRKFKEAILAHRLE
                     QSLDKQQILELYLNEIWLGYRSFGVGAASYNYFGKSLPELTLAESAYLAALPKGPDNY
                     HPIRNKAKAKARRDWILGQMAELGWVSNADAQKAMAEDLVVQAAPRRASYRDADYFVE
                     EVRQRGVATLGPRLSEGGYYMRTTLDPHMQTAARVALMDGLEDYDRRHGWRGAWGHVE
                     ANDPNWEKAARAKSPPSERQGWRAAVVTSTDGRVRLIKNEGEGALVGEDVTWARAGKG
                     LKPGDLIFVEKVEGASTYRLRQIPAVNGALVAIEPYSGRVVALVGGYSFSLSNFNRAT
                     QAMRQ"
BASE COUNT          339 a          615 c          626 g          327 t
ORIGIN      
        1 aaaagggcgc tgagcgcgtg gcgcgggtgc tggaaaaaga cgactggctg atgaaggcca
       61 gcctgccggg ccgtgaccgc gccgtcagtc agatcctcct ggatctctcg cagcgcgcca
      121 ccaagaaccg ctccgccgcc tttgcgcagc tccttctgga gcgggtaggc gaggagactc
      181 cgttgctgcg tcgcagccat cgtgacggag gtttcatcgt cctgctggcg ccggatgttc
      241 cagctgtatt gctggagatg ggcttcatca ccaatcccga ggacgagcgt ctgctgagca
      301 acgccagcag ccgcaaccgc gtcgtcaatg ccgttggcga cgctatcgac gcctacttcg
      361 caacccaggt ccgcaaatcc tgaagcgtgc gcgccgttgg cgcctgcgcc gatgcaggtt
      421 agaggcttga ggcagagtcc gggcgccgag gtgcgaccgc aaaagcgctt acacttttgt
      481 ggaaccggct ctagaggatg agagcagggc gggcgatagt gcgtcgtccg acggaggccg
      541 cgtggatctg aaacccaccg aacgatggat ggccatcgcc ggcgtagcgg tgctttccgt
      601 gatcgccgtg gcgggcttcg cgatcgcgat ctacgccgcg tggctgttcc atgacatgcc
      661 cgatgctggc gagctgcagg actaccgtcc gccgacagca acgcgcgtct atgcgtggga
      721 tggcactctg atcggcgaat actccaagga acgccggatc tttgttccct acgaccagat
      781 tccaccccag ctggcccagg ccttcatggc cgccgaggac cgcaacttct tccgccacag
      841 cggcgtcgat gtcggcggtt tctcgcgcgc gatgatcaag aacgtcagca acgccgcacg
      901 cggtcgtcgt cttgagggcg gatctaccat cacgcagcag gtcgccaaga acgtcctgct
      961 gaccagcgac gctacggtgg gtcgcaagtt caaggaagcg atcctggcgc accgcctgga
     1021 gcagtcgctc gacaagcagc agatccttga gctgtatctc aacgaaatct ggctgggtta
     1081 ccgctctttt ggcgttggcg cggcgtccta caactatttc ggcaagtccc tgcccgagct
     1141 gaccctggcc gagagcgcct atctggcagc cctgcccaag gggccagaca actatcatcc
     1201 gatccgcaac aaggcgaagg ccaaggcccg ccgcgactgg atcctgggtc agatggccga
     1261 gctgggctgg gtgtccaacg cggatgccca gaaggccatg gcggaagacc tcgtggtcca
     1321 ggcggcgcca aggcgcgcct cgtaccgcga tgccgactac ttcgtcgagg aggtgcgcca
     1381 gcgcggcgtc gccaccctcg gcccccgcct cagcgaaggc ggctactaca tgcgcacgac
     1441 cttggatccg catatgcaga cggccgctcg tgtcgccttg atggatggtc ttgaggatta
     1501 cgaccgccgc cacggctggc gcggcgcgtg gggccatgtc gaggccaatg accccaactg
     1561 ggagaaggcc gcgcgcgcca agagcccgcc gtcagagcgc cagggttggc gcgccgccgt
     1621 ggtcacctcg acggatggcc gcgttcggtt gatcaagaac gagggcgaag gcgcgctggt
     1681 tggcgaggac gtgacctggg cccgcgcggg caagggcctc aagccaggcg atctcatttt
     1741 cgtcgagaag gtcgaggggg cttccaccta tcgcctgcgg cagatccccg ccgtcaacgg
     1801 ggccttggtg gccatagagc cctacagcgg ccgtgtcgtt gctctggttg gcggttacag
     1861 cttctcgctg tccaacttca accgcgccac ccaggcgatg cgacagc
//
LOCUS       DAOMEO010000666         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6311_length_1906_cov_2.129660, whole genome shotgun sequence.
ACCESSION   DAOMEO010000666 DAOMEO010000000
VERSION     DAOMEO010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_1906_cov_2.129660"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLH31_13780"
     CDS             <1..490
                     /locus_tag="PLH31_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR90300.1"
                     /translation="KSLGAIGGFCVSNMDDFDVMRVVCRPYMFTASLPPAVVSSTLVA
                     LKRLEESPQLRHRLMANARRLYDGLSELGFQTGPTASPIVAVTMPDQESAIGMWNALL
                     QNGVYLNLALPPATPDSRPLLRTSVSAAHTDEQIDKVLSVFAQLGQALGLIDNQRQRV
                     SA"
     gene            complement(525..1454)
                     /locus_tag="PLH31_13785"
     CDS             complement(525..1454)
                     /locus_tag="PLH31_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90301.1"
                     /translation="MTSQVLAMVEAELRAPAPPHAVAFARQLVSAFEDPVAVLFYGSV
                     LRTGDLDGVMDFYVLTGRARRGWRGVVGRKLWPDVSYHEIEHGGRTLRAKVASMTLAQ
                     FTLAARGREIDTTIWTRFVQPSALVWSSSAEIEPVVINAVAEAAHSAARFAAALGPAE
                     GPSEAYWSALFQQTYKAEFRVERSGREQSILAFDAARYDRLLIACWREQGLLDILAPG
                     SVQPNLTEEARDRFGRAWRVRRRLGKSLNVARLIKAAFTFEGAARYAAWKIERHTGVA
                     VPLTPWREKHPVLAAPGVLWRLWRAQRARRAET"
     gene            complement(1451..>1906)
                     /locus_tag="PLH31_13790"
     CDS             complement(1451..>1906)
                     /locus_tag="PLH31_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HQR90302.1"
                     /translation="NRMGAFHGLSVALTVAGAAAGALMGGARDHWRAGVPLSLSIDDQ
                     PTPDARRFLLMATTLKRLPLGLKPFGPPREGLKILDVDAPPKRLLAALAVLLSGGDAG
                     WLPRLGYRRGDARILRVQTAEAFVLDGEVYDAAGGLTVALGSTLRFLAS"
BASE COUNT          344 a          680 c          605 g          277 t
ORIGIN      
        1 caagagcctg ggcgccatcg gcggcttctg cgtcagcaat atggacgact tcgacgtcat
       61 gcgcgtggtc tgccgcccct acatgttcac cgcctcgctg ccgccggctg tcgtcagctc
      121 gaccctggtc gccctcaagc gcctggagga aagcccgcag ctgcgtcatc gcctgatggc
      181 caacgcccgc cgcctgtatg acggcctgag cgaactgggc ttccagaccg gccccaccgc
      241 cagcccgatc gttgccgtca ccatgcccga tcaggagagc gccatcggca tgtggaatgc
      301 tctgctgcag aacggggtct atctgaacct tgccttgccg cccgccacgc cggatagccg
      361 accgctgctg cggaccagtg tcagcgcagc ccatactgac gagcagatcg acaaggtctt
      421 gtcggtcttc gcgcagctcg gccaggccct gggcctgatc gacaaccagc gccagcgcgt
      481 ttcggcctga cgcaatcagg acgacgcgcc agagggccga gacctcaggt ctcggccctt
      541 cgcgctctct gggcgcgcca tagtctccac aagaccccgg gggccgccag caccggatgt
      601 ttctcgcgcc aaggcgtcag gggcaccgcg acgccggtgt ggcgctcgat cttccacgcg
      661 gcatagcgcg ctgcaccttc gaaggtaaag gcggccttga taagccgcgc gacgttcaag
      721 gatttgccca gccgccgccg cacccgccag gcgcgcccga aacgatcccg cgcttcttcc
      781 gtgagattgg gctgaaccga tccaggggcc aggatgtcaa gaaggccctg ctcgcgccaa
      841 caggcgatca gcagtcgatc atagcgcgca gcgtcgaagg ccaggatcga ttgctcgcga
      901 ccgctgcgct cgacgcggaa ctcggccttg taggtttgct gaaacagggc cgaccagtag
      961 gcttctgatg gcccttcagc gggtcccagc gccgccgcga agcgcgcagc cgaatgggca
     1021 gcctctgcga cggcgttgat gacgacgggc tcaatctccg cgctggagga ccacaccaga
     1081 gcagacggct ggacgaaacg ggtccagatc gtggtgtcga tctcacgccc ccgcgccgcc
     1141 aaggtgaact gggccagggt catggaagcg accttggcgc gcagggttcg cccgccgtgc
     1201 tcgatctcgt gatagctgac gtccggccag agcttgcggc caacgacgcc tcgccagcca
     1261 cggcgggccc ggccagtgag cacatagaag tccatgaccc cgtcaaggtc gccggtgcgc
     1321 aggacggacc cgtagaacag aacagccaca gggtcttcga aggcggagac cagctgtctg
     1381 gcgaaggcca cggcgtgcgg cggcgcaggg gcgcgcaact ctgcctcgac catcgccagc
     1441 acctggctgg tcatgacgcc agaaacctca gggtcgagcc cagagcaaca gtcagaccgc
     1501 ccgctgcgtc atacacctcg ccatccagca caaaggcctc ggccgtctgg acgcgcagaa
     1561 tccgtgcgtc acctcgtctg taaccgagcc tgggcaacca gccagcgtcg ccgccgctca
     1621 acagaacagc cagcgccgcc agcagtcgct tgggaggcgc gtccacgtcg aggatcttca
     1681 acccctcgcg cggcggtccg aagggcttca gccccagcgg caggcgcttc agcgtcgtgg
     1741 ccatcagcag gaaccggcgc gcatccggcg tgggttgatc atcgatggaa aggctcaaag
     1801 gcacgccggc ccgccagtgg tcgcgcgcgc cgcccatcag cgccccggcc gccgcccccg
     1861 ccacggtcag ggccaccgac aggccatgga aggcgcccat acgatt
//
LOCUS       DAOMEO010000667         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6312_length_1906_cov_1.781740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000667 DAOMEO010000000
VERSION     DAOMEO010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6312_length_1906_cov_1.781740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLH31_13795"
     CDS             <1..1100
                     /locus_tag="PLH31_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90303.1"
                     /translation="VSGKGKYSNMLPSLDFQVDLTENVVGRFSFSRTIARPDYGNLFA
                     AQTAGAPNRPVANGAIPLGTSGNPDLEPLISDNFDVSVEWYYKPSSYVSAGFFEKRVN
                     NFIGTGTVTQTLFDLRDPSSGAAGTRSGDARASLTAIGANQTDVNLFTMTALIQQTGS
                     VAAATAAFQANRGPSGDLNQAYADQILAAFDIEPNAQDPLFNFNVARPINNRTGKIHG
                     FEVAAQHFFGDTGFGVAGAYTMVRGDVDFDIASDPGQDQFALLGLSDTANATLIYDKN
                     GLSARLAYNWRDRFLQQTNRGGSRNPVFVEHFGQLDFNISYDVTEKLAVSLEGLNLTR
                     ESLRTYGRDVNQLWFAQELDRRFLLGARYRF"
     gene            1227..1892
                     /locus_tag="PLH31_13800"
     CDS             1227..1892
                     /locus_tag="PLH31_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HQR90304.1"
                     /translation="MINRVLLNNINLALVLPTEFEEVQREYPILLRKDQNGDYQAVAL
                     LGLDRDENLFLDGETWRARYVPAVQQRGAFSIALQDPGDGSEPAPMIHVDLDHPRIGS
                     GEGQPVFLPAGGNAPYLQQVMRVLGTIYDGLDVAPQMYAAFAALDLIAPVEINIALDG
                     GASYDLPDFHTIDADRLAALSGIDLERMHRAGLLRAAQWIISSLGNIQHLVELKNRRL
                     ATA"
BASE COUNT          342 a          623 c          580 g          361 t
ORIGIN      
        1 cggtttccgg caagggcaag tacagcaaca tgttgccgtc cttggacttc caggtggatc
       61 tgaccgaaaa cgtggtcggt cgcttctcct tcagccgcac gatcgcgcgt ccggactacg
      121 gcaacctgtt cgccgcccag acggccggcg cgcccaaccg cccggtcgcc aacggcgcca
      181 tcccgctcgg caccagcggc aaccccgacc tggaaccgct gatctccgac aacttcgacg
      241 tgtccgtgga gtggtactac aagcccagca gctatgtttc ggcgggcttc ttcgaaaagc
      301 gcgtgaacaa cttcatcggt acgggcaccg tcactcagac cctctttgac ctgcgtgacc
      361 ctagctccgg cgcggcaggc acccgttcgg gcgacgcccg ggccagcctg accgcgatcg
      421 gcgccaacca gaccgacgtg aacctcttca cgatgacggc cctgatccag cagaccggat
      481 cggtggcggc ggcgacggcg gcgttccagg ccaatcgcgg tccgagcggc gacctgaacc
      541 aggcctatgc cgatcagatc ctggcggcct ttgatatcga gccgaacgct caggatccgc
      601 tgttcaactt caacgtggcg cgtccgatca acaaccggac cggcaagatc cacggctttg
      661 aagtcgctgc tcagcacttc ttcggcgaca ccggcttcgg tgtggcgggc gcctatacca
      721 tggttcgcgg cgacgtcgac ttcgacatcg cgtcggatcc tggccaggac cagttcgccc
      781 tgctgggtct cagcgacacg gccaacgcca ccctgatcta cgacaagaac gggctctcgg
      841 ctcgcctggc ctacaactgg cgcgaccggt tcctgcaaca gaccaaccgt ggtggttcgc
      901 gcaacccggt gttcgtcgag cacttcggcc agctggactt caacatcagc tacgatgtca
      961 ctgaaaagct ggcggtttcg ctggaaggtc tcaacctgac gcgcgaaagc ctgcgtacct
     1021 acggacgtga tgtgaaccaa ctctggttcg cccaggagct ggacaggcgc ttcctcctcg
     1081 gcgctcgcta ccgcttctag accgaacgtc tggagggcga ggggaaaggt cgctgttcgc
     1141 agcggccttt ttcttcgaca acggcttagg atcgggtctg acttggactg tctgaagtca
     1201 ggcctgatcc ggctcgaaag gtgcgtatga tcaacagggt gctgcttaac aacatcaatc
     1261 tggccttggt gctgcccacc gagttcgagg aggtccagcg cgagtacccc atcctgctgc
     1321 gcaaggacca gaacggcgac tatcaagccg tggccctgct ggggctcgat cgcgatgaga
     1381 acctgttcct cgatggggaa acctggcgcg cgcgctatgt gccggcggtt cagcaacggg
     1441 gcgccttttc catcgcgcta caggatccgg gcgatggctc cgagccagcg ccgatgatcc
     1501 atgtcgatct ggatcacccg cgtatcggtt ctggcgaggg ccagcctgtg tttctgccgg
     1561 cggggggtaa tgcgccctat ctgcagcagg tgatgcgtgt gctgggaacg atctatgacg
     1621 ggctggacgt ggcgccccag atgtacgctg cctttgccgc cctggacctg atcgccccgg
     1681 tcgagatcaa tatcgcgctg gacggcggag cgtcgtacga tctgcccgat ttccacacca
     1741 tcgacgccga ccggttggcg gccttgtctg gcatcgacct ggaacgcatg catcgcgcgg
     1801 gccttcttcg cgcggctcaa tggatcatct cgtcgcttgg caatattcag catctggtcg
     1861 agctgaagaa ccggcggttg gcaaccgcat gaccacgccc gcgcga
//
LOCUS       DAOMEO010000668         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6324_length_1902_cov_2.497564, whole genome shotgun sequence.
ACCESSION   DAOMEO010000668 DAOMEO010000000
VERSION     DAOMEO010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_1902_cov_2.497564"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLH31_13805"
     CDS             <1..277
                     /locus_tag="PLH31_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HQR90305.1"
                     /translation="VDEVGLDSLDRRYLRALIEHYGGGPAGVETIAYAIAESRDAVED
                     VIEPYLMQQGFIQRTPRGRVACGKAYLHLGLKAPAAPAGQAGLFGEE"
     gene            282..>694
                     /locus_tag="PLH31_13810"
     CDS             282..>694
                     /locus_tag="PLH31_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQR90306.1"
                     /translation="MRAIRFVTAVQAAACLGVSLVAISVAGAGRNALVMQTLAAVIAL
                     GLALVVARFWRKVGWRYWLLVAACALALVWTLMTGVTLEGARRWLAVGPVQLHTAFLA
                     LPLAVWAFARGFEDRRVAPLAAGLSFALLYQHDAAS"
     assembly_gap    695..704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <705..1125
                     /locus_tag="PLH31_13815"
     CDS             <705..1125
                     /locus_tag="PLH31_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQR90307.1"
                     /translation="AIGAATLVQRPRQMVPWAVVAVAAVFAYAAWTEPDTLPAVPYVE
                     GLLAANFATSPVVGALAAILMFSLPAPFLLVAASRPERRAEALALAALWAGWIFGNLF
                     GNYPAPVLGYGAAPILAWGLSIGLIFAETANQGDPAP"
     gene            complement(1134..1409)
                     /locus_tag="PLH31_13820"
     CDS             complement(1134..1409)
                     /locus_tag="PLH31_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR90308.1"
                     /translation="MRRIIWTVEAVSNLESIFAYVSAFNASAAVRLARRLQSAADSLA
                     THPDRGRETVRGLRELTIIHPYLIRYRVKGDTVLILRIRHGARDVEA"
     gene            complement(1409..1585)
                     /locus_tag="PLH31_13825"
     CDS             complement(1409..1585)
                     /locus_tag="PLH31_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQR90309.1"
                     /translation="MKIEPAIFDETDDAAEAAADARAEADISAGRVISHEAVSRWLKS
                     WSDGAPTPAPQSGD"
     gene            1744..>1902
                     /locus_tag="PLH31_13830"
     CDS             1744..>1902
                     /locus_tag="PLH31_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQR90310.1"
                     /translation="MTTPDPTAGVFDGREHQLPVRIYYEDTDFSGIVYHANYLRYFER
                     GRSDFFRLI"
BASE COUNT          268 a          651 c          625 g          348 t
ORIGIN      
        1 agtggacgag gtcggcctcg acagcctcga ccgccgctac ctgcgcgccc tgatcgagca
       61 ctatggcggc ggcccggccg gggtcgagac catcgcctac gccatcgccg agtcccgcga
      121 cgcggttgaa gacgtcatcg agccctatct gatgcagcag ggcttcatcc agcgcacccc
      181 gcgcggccgg gtggcctgcg gcaaggccta tttgcacctt gggctgaagg ctccggccgc
      241 gccggcgggg caggcgggtt tgttcgggga ggaatagggc catgcgcgcc atccggttcg
      301 tgacggccgt ccaggcggcg gcctgtcttg gcgtctcgct ggtagcgatc agcgtggccg
      361 gcgccggccg gaacgcgctg gtgatgcaga ccctggccgc cgtcatcgcc cttggtctgg
      421 ccctggttgt cgcgcgcttc tggcgaaagg tggggtggcg gtactggttg ctggtcgccg
      481 cctgcgccct ggccctggtc tggaccctga tgaccggcgt cacgctggag ggcgcgcggc
      541 gctggctggc cgtggggccg gtgcaactgc acaccgcctt cctggccctg cccctggccg
      601 tgtgggcgtt cgccagaggt ttcgaggacc ggcgcgtcgc gcctctggcc gcgggcctga
      661 gttttgcgct gctctaccag catgacgcgg cctcnnnnnn nnnnggccat cggcgcggcg
      721 accctggtcc agcgcccgcg ccagatggtc ccgtgggccg tggtcgccgt cgccgccgtg
      781 ttcgcctacg ccgcctggac cgaacccgac accctgccgg ccgtgcccta tgtcgagggc
      841 ctgctggccg ccaacttcgc gaccagcccc gtggtcggcg ccctggccgc gatcctcatg
      901 ttcagcctgc ccgcgccgtt tctgctggtc gccgccagcc gacccgagcg gcgcgccgag
      961 gccctggccc tggcggcgtt gtgggcgggc tggattttcg gcaacctgtt tggcaactat
     1021 cccgcgccgg tcctgggtta cggcgcggcg ccgatcctgg cctggggcct gtcgatcggg
     1081 ctcatattcg ccgaaaccgc caaccagggc gatccggcgc catgatctag gcctcaggcc
     1141 tcgacgtccc gagcgccgtg acgaatccgc aggatgagaa cggtatcgcc cttgaccctg
     1201 tagcggatca ggtaggggtg aatgatcgtg agctctcgca gtccacggac ggtctcacgt
     1261 ccccgatccg gatgcgtggc caggctgtca gccgccgatt gaaggcgtcg cgccaaacga
     1321 acagccgcgg acgcgttgaa cgctgacacg taggcgaaga tgctttcgag gttggatacg
     1381 gcttccaccg tccagataat ccgccgcact agtcgccaga ttgaggcgcc ggcgtgggtg
     1441 cgccatcgga ccacgatttc agccagcggc ttacggcttc atgactgata acccggcctg
     1501 ccgaaatatc agcttcggcg cgcgcgtcgg cggcggcttc agcggcatcg tccgtttcat
     1561 cgaagatggc gggttcgatc ttcatggcca gaatttatca tggcgaggcg aaagtccata
     1621 atcaattcca gattgaattt tcacctttgc gcctttgacg gcggtaccga tttcgccagc
     1681 gggcttatcg cccttgcctg accaacctat actccccgga ccccagggcg cgccggagcg
     1741 tagatgacca ccccagaccc caccgccggc gtcttcgacg gccgcgagca ccagctgccc
     1801 gtccgcatct actacgaaga caccgacttt tccggcatcg tgtatcacgc caactacctg
     1861 cgctatttcg agcgtgggcg gagcgatttc tttcggctga tc
//
LOCUS       DAOMEO010000669         1902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6325_length_1902_cov_2.442880, whole genome shotgun sequence.
ACCESSION   DAOMEO010000669 DAOMEO010000000
VERSION     DAOMEO010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6325_length_1902_cov_2.442880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PLH31_13835"
     CDS             <1..1217
                     /locus_tag="PLH31_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HQR90311.1"
                     /translation="LLLYVEHLNQDRLEQDVATVWPGTSLIADYLGCTESNARAHRRA
                     LEAAGFMVRDYTRANRPAGVEAYDLAPLVARLEEMEAADLAVREAAQARRAGYSEPVV
                     FSERTTARAPRFTHLEQSHQNGSSSVRGKDAPPARHESYQRRPPPAENGEVRKSSGKR
                     QSTGQESAICSPGGASGFSSAFSEGSAPAEMVRQELRAAFQVSPRIAALVGPAAVADP
                     ASVTPADLARVAAAVDDLLPERERNNDQTFAWALKRHGVRAIAMLAIAIEDPDVRSPC
                     KYFGRMASYEPKGAPDLRLNFARVLKAKGDIPPAEPPPPLMSAPGVDDPVWQKIAEPL
                     RRLVREGAYGSWFGRIGFHGIDEGVLTLSTLSQIAADRIKRDYVDAILHAAEVAEVYV
                     DRVIITLRKDRR"
     gene            1214..1750
                     /locus_tag="PLH31_13840"
     CDS             1214..1750
                     /locus_tag="PLH31_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQR90312.1"
                     /translation="MIRRLSLMVLAIGLAAAAPAQVTGPARVVDGDTFSVGAERVRLW
                     GVDAPEGRQVCQNDQGQGYACGDVARDQLVGLIGGRAVRCEVRDRDPYGRAVARCLAG
                     ATDLGEAMVRAGWAVDDIQFSRGAYASAEVEARRAPSAPGAGRTPSAGLRHQGQHQRQ
                     GPPDLPCPGPRGLRRDPY"
BASE COUNT          311 a          618 c          657 g          316 t
ORIGIN      
        1 ccctgctcct ctatgtcgag catctgaacc aggatcggct tgaacaggac gtggccacgg
       61 tgtggcccgg gaccagtttg atcgccgatt atctgggatg cacggagagc aatgcccggg
      121 cgcaccgacg cgcgctggaa gccgccggct tcatggtgcg agactacact cgcgccaatc
      181 ggccggcggg cgtcgaggcc tatgacctcg cgcccctcgt ggcgcggctg gaggagatgg
      241 aggctgcgga tctcgccgtc cgcgaagctg ctcaggcccg gagggccggc tattccgaac
      301 ctgtcgtctt tagtgaaaga actaccgccc gggcgccaag attcactcac ctagaacagt
      361 cccatcagaa cggcagttct tctgtacgag gaaaggacgc gcctccagcg cgacatgaat
      421 cttatcagcg gcggcctccg cccgccgaga acggcgaagt ccgaaaatcc tcgggcaaac
      481 gccagagcac cggacaagag agcgccattt gctctccggg gggagcaagc ggtttctcca
      541 gcgccttttc ggaggggtcc gcgcccgcgg aaatggtccg ccaggagctt agggctgcct
      601 tccaggtctc tccccgcatc gctgcgctgg tgggacctgc cgccgtggcc gacccggcca
      661 gcgtaacgcc tgccgatctc gctcgggtgg cggccgccgt tgacgatctg ctgcccgaac
      721 gtgaacgaaa caacgaccag accttcgctt gggcccttaa gcggcacggg gtccgcgcca
      781 tcgccatgct ggcgattgcg atcgaggatc ccgacgttcg cagcccgtgc aaatatttcg
      841 gccggatggc ctcctacgag ccgaaggggg cgccggacct gcggctcaac ttcgcccggg
      901 tgcttaaggc caagggcgac attccgccgg ctgaaccgcc gccgcccttg atgtcggcgc
      961 cgggggtcga tgaccccgtc tggcaaaaga tcgctgagcc gctccgccgg ctagtccgcg
     1021 agggcgccta tggcagctgg ttcggccgga tcggcttcca tgggatcgat gagggggtgc
     1081 tgaccctgtc gacgctcagc cagatcgccg ccgaccggat caagcgcgac tacgtcgacg
     1141 cgatcctgca cgcggccgag gtcgccgagg tctatgtcga ccgggtgatc atcaccctgc
     1201 gaaaggaccg gcgatgatcc ggcggttgag ccttatggtg ctggccatcg ggttggcggc
     1261 cgccgcgccg gcgcaggtga cgggcccggc ccgtgtcgtc gatggcgaca ccttcagcgt
     1321 cggcgccgag cgggtccgcc tttggggcgt cgatgcgcca gaaggccgac aggtctgcca
     1381 gaacgaccag ggccaaggct acgcctgcgg cgacgtcgcg cgcgaccagc tcgtcggcct
     1441 gatcggcggg cgggcggtgc gctgtgaggt gcgcgaccgt gatccttatg gccgggcggt
     1501 tgctcggtgc ttggccggag cgaccgatct gggcgaggcg atggttcgag cgggatgggc
     1561 ggtcgacgat atccagttca gccgcggcgc ctatgcgtca gccgaggtcg aggcgcgaag
     1621 ggcgccaagc gctcccggcg ccggccgcac cccctcagcc gggttgcgtc atcaagggca
     1681 acatcaacgc caagggccgc cggatcttcc atgtcccggg ccaagaggat tacgccgcga
     1741 cccgtattga cccttcgaac ggggagcgct ggttctgttc agcggctgaa gcacgggcgg
     1801 ccggttggac ggccgctact cgatgatcga agggttcaag cgtggcagcg tcactttgct
     1861 gcggatcctg cccacgtgag gttctcgagc ttgatgttaa gt
//
LOCUS       DAOMEO010000670         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6345_length_1897_cov_2.128122, whole genome shotgun sequence.
ACCESSION   DAOMEO010000670 DAOMEO010000000
VERSION     DAOMEO010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_1897_cov_2.128122"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLH31_13845"
     CDS             complement(<1..373)
                     /locus_tag="PLH31_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90313.1"
                     /translation="MHGTTKPGDLDVMRRSFPLALTCALLAGPALAADPGTTQRGDTL
                     RDAVTAPLEDLNLKRKDIPAVLNRAVADPYDITGLDRCEPIAAEIGRLDAALGPDLDE
                     APPPDERSRGKKVVDGAWQAGV"
     gene            complement(403..>1897)
                     /locus_tag="PLH31_13850"
     CDS             complement(403..>1897)
                     /locus_tag="PLH31_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQR90314.1"
                     /translation="FSAPRSDLVPNTAAYENEPLVKATGFREYDARWLFGPDINLLGI
                     QALGLGLGTYIHELGLSKIVVGHDFRSYSSSIKNALILGLISAGCEVHDIGLALSPTA
                     YFAQFDLDIPCVAMVTASHNENGWTGVKMGAQRPLTFGPDEMGRLRDIVLGGKFVERD
                     GGKLIRVEGEAERFIADVAKRASITRPLKVIAACGNGTAGAFAVDALQRMGVSDVVGM
                     DTELDYTFPKYNPNPEDVEMLHSMAAAVREHKADLAFGFDGDGDRCGVVDDEGEEIFA
                     DKIGLMLARDLAPLNPGATFVVDVKSTGLYATDPILSAHGCKVIYWKTGHSYIKRKSA
                     EVGALAGFEKSGHFFMNGELGYGYDCGLTAAAAILAMLDRNPGQKLSDMRKALPVAFT
                     SLTMSPHCGDEVKYGVVDDVVKEYQDLFAAGGSILGRRVTEVITVNGVRVHLEDGSWV
                     LVRASSNKPEIVVVVESTRSEDDMRALFRQEVKPRLGDRVGKYNQEI"
BASE COUNT          295 a          609 c          670 g          323 t
ORIGIN      
        1 ccaccccagc ctgccaggcg ccgtcgacca ccttcttgcc cctggagcgc tcgtcgggcg
       61 gcggcgcttc atccagatcc gggcccaggg cggcatccag gcggccgatc tcggcggcga
      121 tcggttcgca gcggtccagg ccggtgatgt catagggatc tgcgaccgcg cggttcagca
      181 cggccgggat atccttgcgc ttgaggttca ggtcctccag cggcgcggtg acggcgtcgc
      241 gcagggtgtc gccgcgctgg gtcgtgccgg gatcggcggc cagggccggt ccggcgagca
      301 gggcgcaggt cagggcaaga gggaacgagc gacgcatcac gtcgagatcg cccggctttg
      361 tggttccgtg caagcggaat gctcggacgg cgccgctcgg tcttagattt cctggttgta
      421 cttgccgacc cggtcgccca ggcgcggctt cacttcctgg cggaacaggg cgcgcatgtc
      481 gtcctcggag cgggtgctct cgaccacgac cacgatctcg ggcttgttgg acgaggcgcg
      541 caccagcacc catgagccgt cttccagatg cacgcgcacg ccgttgacgg tgatgacctc
      601 ggtgaccctg cggcccagga tggagccgcc ggcggcgaac aggtcctgat attccttcac
      661 cacgtcatcg acgacgccgt acttcacctc gtcgccgcag tgcgggctca tggtcaggga
      721 ggtgaaggcc accggcaggg ccttgcgcat gtccgacagc ttctggccgg ggttacgatc
      781 cagcatggcg aggatggcgg cggcggcggt caggccgcag tcatagccgt agcccagctc
      841 gccgttcatg aagaagtggc cgctcttctc gaagccggcc agggcgccga cctcggcgct
      901 cttgcgcttg atgtagctgt ggccggtctt ccagtagatc accttgcagc cgtgtgcgct
      961 gaggatcggg tcggtggcgt agaggccggt ggatttcaca tcgaccacga aggtcgcgcc
     1021 ggggttcagc ggggccaggt cgcgggccag catgaggccg atcttgtcgg cgaagatctc
     1081 ctcgccctcg tcgtcgacca cgccgcagcg gtcgccatcg ccgtcgaagc cgaaggcgag
     1141 gtccgccttg tgctcgcgca cggcggcggc catggagtgc agcatctcca cgtcttcggg
     1201 gttcgggttg tacttcggga aggtgtagtc gagctcggtg tccatgccga ccacgtcgga
     1261 gacgcccatg cgctgcaggg cgtcgaccgc gaaggcgccg gccgtgccgt tgccgcaggc
     1321 ggcgatcacc ttcagcggcc gggtgatgct ggcgcgcttg gcgacgtcgg cgatgaagcg
     1381 ctcagcctcg ccctcgacgc ggatcagttt gccgccgtca cgctccacga acttgccgcc
     1441 cagcacgatg tcgcgcagcc gacccatctc gtcgggacca aatgtgagag ggcgttgcgc
     1501 gcccatcttc acgccggtcc agccgttctc gttatggctg gcggtgacca tggccacgca
     1561 ggggatgtcc aggtcgaact gggcgaaata ggcggtgggc gacaaggcca ggccgatgtc
     1621 gtgcacctcg cagccggccg agatcaggcc caggatcagg gcgttcttga tcgacgacga
     1681 ataggagcgg aagtcgtggc cgaccacgat cttcgacagg cccagttcgt ggatgtaggt
     1741 gccaaggccc aggcccaagg cctgaatgcc cagcaggttg atgtccggcc caaacagcca
     1801 gcgcgcgtcg tattcacgaa agcccgtggc cttgaccagc ggctcgttct cataggcggc
     1861 ggtattggga acgaggtcgg agcggggagc ggagaac
//
LOCUS       DAOMEO010000671         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6348_length_1895_cov_2.664674, whole genome shotgun sequence.
ACCESSION   DAOMEO010000671 DAOMEO010000000
VERSION     DAOMEO010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6348_length_1895_cov_2.664674"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1807
                     /gene="ffh"
                     /locus_tag="PLH31_13855"
     CDS             287..1807
                     /gene="ffh"
                     /locus_tag="PLH31_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQR90315.1"
                     /translation="MFDGLTERLSGVFDRLGGRGVLSDKDIDEALREVRVALLEADVA
                     LPVVKDFISKAKELASGEAVIRSVKPADQVVKIVYDGLVDMLGGEEPTGLNLALNPPS
                     VILMTGLQGSGKTTTTGKLALRLSRNERKKVLVASLDTRRPAAMEQLAMLAKQVEVES
                     LPIVAGQSAVDIAKRAMSAAKLGGYDVLILDTAGRTTLDEAMMSEAAEIARIAQPSET
                     LLVADSLTGQDAVRTAKAFHERLPLTGLILTRADGDGRGGAALSMRHVTGLPIKFLGA
                     GEKIDALDVFDARRVAGRILGQGDVVALVERAAQDLDQAEAERMAKKLAKGQFDLNDL
                     SSQLSQMQKMGGMEGIMGLLPGVQKVKKQIAESGIDDSIFRRQQAIISSMTKLERRKP
                     DILAASRKRRIAAGAGVDVAEVNRLLKQHRQMADMFKAMSKDGGKSLAKMAGMMGGGD
                     MSRLKAMGGGKVAQPQQNSAGGLPGLPGLGGGGDAKPNLLSGLGGGLPPGFNPFKK"
     gene            1832..>1895
                     /locus_tag="PLH31_13860"
     CDS             1832..>1895
                     /locus_tag="PLH31_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQR90316.1"
                     /translation="MLKIRLARGGAKKRPYYSIVV"
BASE COUNT          333 a          638 c          635 g          289 t
ORIGIN      
        1 ctacgcgatc ctgcgcaaga acggcgtcga ggtcggcaag cgcgactatc tgggcatggc
       61 ctgatttcag gctggcggcc cgtctgggcg ggtcgccttt tgactccctc gccttttctg
      121 tgcataaggc ccgccttccg gcgcttcatc agcagcgcct ctaccgtcac gaccccggcc
      181 tgttagcgca ggatccatcc ggacgaggac ggcgaggacc cccatcgacg tgatcgtcca
      241 tgggggctga ttgcgtttgg agctttgggt tcaaacaggg ttcgacatgt tcgacggcct
      301 tacagagcgg ctctccggcg tcttcgaccg cctcggcggt cgcggcgtgc tgtccgacaa
      361 ggacatcgac gaggcgctgc gggaggtccg cgtcgccctg ctggaagccg acgtcgccct
      421 gccggtggtc aaggacttca tcagcaaggc caaggaactg gcctcgggcg aggcggtcat
      481 ccgctcggtc aagccggccg atcaggtggt caagatcgtc tatgacggtc tggtcgacat
      541 gctgggcggc gaagagccga ctggcctgaa cctggctctg aacccgccgt cggtgatcct
      601 gatgaccggc ctgcagggct cgggcaagac caccaccacc ggcaagctgg ccctgcgcct
      661 gagccggaac gagcgcaaga aggtgctggt cgcctcgctc gacacgcgtc gtcccgccgc
      721 catggaacag ctggccatgc tggccaagca ggtcgaggtc gagagcctgc cgatcgtggc
      781 cggccaaagc gccgtcgaca tcgccaagcg cgccatgagc gccgccaagc tgggcggtta
      841 tgacgtcctg atcctcgaca ccgccggccg caccacgctg gacgaggcga tgatgagcga
      901 agcggcggaa atcgcccgca tcgcccagcc gtccgagacc ctgctggtcg ccgacagcct
      961 gaccggtcag gatgccgtcc gcaccgccaa ggccttccac gaacgcctgc cgctgaccgg
     1021 cctgatcctc acccgcgccg atggcgatgg ccggggcggg gcggcgctgt cgatgcgcca
     1081 tgtcaccggc ctgccgatca agttcctggg cgccggcgag aagatcgacg cgctggacgt
     1141 gttcgacgcc cgccgcgtgg ccggccgcat cctggggcaa ggcgacgtcg tcgctctggt
     1201 ggaacgcgcc gcccaggacc tggaccaggc cgaagccgag cgcatggcca agaagctggc
     1261 caagggccag ttcgacctca acgacctgtc gtcccagctg tcgcagatgc agaagatggg
     1321 cggcatggaa ggcatcatgg gcctgctgcc gggcgtccag aaggtcaaga agcagatcgc
     1381 cgaaagcggc atcgacgaca gcatcttccg ccgccagcag gcgatcatca gctcgatgac
     1441 caagctggag cgcaggaagc ccgacatcct ggccgcctcg cgcaagcgcc gcatcgcggc
     1501 cggcgccggg gtggacgtgg ccgaggtcaa ccgcctgctg aagcagcacc gccagatggc
     1561 cgacatgttc aaggccatgt ccaaggacgg cggcaagagc ctggccaaga tggctggcat
     1621 gatgggcggc ggcgacatgt cgcgcctcaa ggctatgggc ggcggcaagg tcgctcagcc
     1681 ccaacagaat tccgcgggag gcctgccggg tcttcccggt ctcggcggtg gcggcgacgc
     1741 caagccgaac ctcctttccg gcctgggcgg cggcctgccg cccggtttca acccgttcaa
     1801 gaaatagaac tcgaactcaa ggactaccga tatgctgaag atccgtctcg cccgtggcgg
     1861 cgccaagaag cgcccgtact actcgatcgt cgttg
//
LOCUS       DAOMEO010000672         1894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6355_length_1894_cov_2.512779, whole genome shotgun sequence.
ACCESSION   DAOMEO010000672 DAOMEO010000000
VERSION     DAOMEO010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_1894_cov_2.512779"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLH31_13865"
     CDS             <1..1011
                     /locus_tag="PLH31_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR90317.1"
                     /translation="VQNAAVAAGLAIATGSEPTPLPGVTVDNVRIVDSTGALSLPEVP
                     KSLVVIGAGVIGLELGSVWRRLGADVTVVEYLDRICPGMDDETAKVFQRSLTKQGFKF
                     QLGAKVTGAKASAKGVELSFEPVAGGEAQTVSADYVLVAIGRRPYTEGLGLETVGVVP
                     DKRGMIANDHFKTTAPGVWVVGDVTSGPMLAHKAEDEGVACIELIAGKAGHVNYGIIP
                     GVIYTKPEVATVGKTEEELKAEGVAYKVGKFPFTANSRAKVNHENEGQVKVLADAKTD
                     RILGVHAVGPNVGDMIAEYCVAMEFGGSSEDVARTSHPHPTRSEAMRQAAMGVEGWVM
                     QA"
     gene            1093..1377
                     /locus_tag="PLH31_13870"
     CDS             1093..1377
                     /locus_tag="PLH31_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90318.1"
                     /translation="MLLSALLLAAAVQAAPIAPEPPGTLKPPGVVHGRIMRPARRTCR
                     PMQVVPVDQPGEDGSPAPNGMRSGGLQYAVMRSIDGCSIPTPIRQVRPAR"
     gene            complement(1382..>1595)
                     /locus_tag="PLH31_13875"
     CDS             complement(1382..>1595)
                     /locus_tag="PLH31_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HQR90319.1"
                     /translation="QMRKGARDLRAEAVRLRDPAYRARQIADNRGRGNQVTDAELRAL
                     ADQMPAQADDMERQAERLAAEARKSS"
     assembly_gap    1596..1662
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1663..1878)
                     /locus_tag="PLH31_13880"
     CDS             complement(<1663..1878)
                     /locus_tag="PLH31_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HQR90320.1"
                     /translation="MAANARAHAAEARAQAMEARRQADAGRAAADIARMAADQARVRA
                     DQARVEHDRARLGDEQARMIGERAREEA"
BASE COUNT          260 a          628 c          636 g          303 t
ORIGIN      
        1 gtccagaacg cggcggtcgc cgccggtctg gcgatcgcca ccggctcgga gcccacgccg
       61 ctgcccggcg tcaccgtcga caacgtccgc atcgtcgact cgaccggcgc cctgtcgctg
      121 ccggaagttc ccaagagcct ggtcgtgatc ggggccggcg tcatcggcct tgagctgggt
      181 tcggtctggc gtcgtctggg cgcggacgtc accgtggtcg aatacctcga ccgcatctgc
      241 ccgggcatgg atgacgagac cgccaaggtg ttccagcgca gcctgaccaa gcagggcttc
      301 aagttccagc tgggcgccaa ggtcaccggc gccaaggctt cggccaaggg cgtggagctg
      361 tcgttcgagc cggtcgccgg tggcgaggcc cagacggttt cggccgacta tgtgctggtg
      421 gccattggtc gccgtccgta cacggaaggt ctgggcctgg agacggtcgg cgtcgttccc
      481 gataagcgcg gcatgatcgc caatgaccac ttcaagacca cggctccggg cgtctgggtc
      541 gtcggcgacg tgacctcagg tccgatgctg gcgcacaagg ccgaggacga gggcgtggcc
      601 tgtatcgagc tgatcgcggg caaggcgggt cacgtgaact acggcatcat cccgggcgtc
      661 atctacacca agccggaagt ggccacggtg ggcaagaccg aggaagaact gaaggccgaa
      721 ggcgtcgcct acaaggtcgg caagttcccg ttcacggcca atagccgcgc caaggtcaat
      781 cacgagaacg aaggccaggt gaaggtgctg gccgacgcca agaccgatcg catcctgggc
      841 gttcacgccg tgggtcccaa tgtgggcgac atgatcgccg aatactgcgt ggccatggag
      901 tttggcggct cgtcggaaga cgtggcccgc acctcccacc cccaccccac ccgctcggaa
      961 gccatgcgcc aggcggccat gggggtcgag ggctgggtga tgcaggcctg atcggctcct
     1021 gacacagagt taacgcgtgc gccggttcaa tctggcgtag agtggcgggg tttcggcaaa
     1081 cggagccccg ccatgctttt gtccgctctc ctgctcgcgg ccgctgtcca ggccgcgccg
     1141 atagcgcccg aaccgcccgg cacgctgaag ccgcccggcg ttgttcatgg ccggatcatg
     1201 cgccccgcgc gccggacctg ccgtcccatg caggttgtgc ctgtcgatca gcccggtgaa
     1261 gacggttccc ccgcgccgaa cgggatgcgg tccggcggtc tgcagtacgc ggtgatgcgc
     1321 tcgatcgacg gctgttcgat tccgaccccg atccgccagg tcagaccagc ccgataaccg
     1381 ctcagctcga cttgcgcgct tctgcggcca gtcgttcggc ctggcgctcc atgtcgtcgg
     1441 cctgggcggg catctggtcc gccagggcgc gcagctcggc gtcggtgacc tgattgcctc
     1501 gcccgcggtt gtcggcgatc tgcctggcgc gataggcggg gtcgcgcaga cgcacggctt
     1561 cggcgcgcag gtctctcgcc cccttgcgca tctgtnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcctcct cgcgggcgcg
     1681 ctcgccgatc atgcgggcct gttcgtcccc aagccgcgcc cggtcgtgtt caaccctggc
     1741 ctggtcggcg cgaacccgcg cctggtcggc ggccatgcgg gcgatgtcgg ctgcagcgcg
     1801 gccggcgtcg gcctggcgcc gcgcctccat ggcttgagcc cgggcctccg cggcatgggc
     1861 gcgagcgttg gcggccatcg ccctctcggc tggc
//
LOCUS       DAOMEO010000673         1891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6365_length_1891_cov_1.783769, whole genome shotgun sequence.
ACCESSION   DAOMEO010000673 DAOMEO010000000
VERSION     DAOMEO010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_1891_cov_1.783769"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLH31_13885"
     CDS             <1..286
                     /locus_tag="PLH31_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR90321.1"
                     /translation="LKDAPILLLDEATSALDTESEAQVQSALERLMAGRTTILIAHRL
                     STVKGADRIYVIDKGQVVETGTHAELVRAKGLYARLAKAQDLDHLPGDAA"
     assembly_gap    352..361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <362..993
                     /locus_tag="PLH31_13890"
     CDS             <362..993
                     /locus_tag="PLH31_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR90322.1"
                     /translation="REGQAIADDVQAQALAERSGVILALWHSRVPVGPATWPQGADKP
                     EIRVLVSQSRDGEFIARVIARLGLPSIRGSSLKKTDTAKNKGGEQAFRDMVKWVKDGG
                     AMAITPDGPRGPVEVLQKGAVALARVSGAPVLFVGVAMKPCIRLGTWDRTIIPLPFAK
                     AAMVWDGPITAGRDDDPDVLAEAWGARLSAVSRRAEQIVGEDDGAPVRH"
     gene            1024..>1891
                     /locus_tag="PLH31_13895"
     CDS             1024..>1891
                     /locus_tag="PLH31_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQR90323.1"
                     /translation="MPHDHSGHAHDHHGHDHGHDHAHDHGHGHSHGHGHGHHHHVPKD
                     FGRAFAIGTALNLGFVLVEFGAGLLTQSLALLADAGHNLSDVLGLVLAWGAATLAKRA
                     PSGRHTYGLRKGTVLASLGNAALLLAATGAIAWEAIRRFAAPTPVESGPVMIVATIGI
                     IINTATALMFMRGSKDDLNARGAFLHMAADAAVSAGVVLAALLMMLTGWLWIDSVTSL
                     LIVAVIVLGTWGLLRDSLDMALDAAPRGIDPAAVRDWLAARPGVIEVHDLHVWAMSTT
                     ETAMTAHVVRPTG"
BASE COUNT          268 a          668 c          655 g          290 t
ORIGIN      
        1 cctgaaggat gcgccgatcc tgttgctgga tgaggccacc agcgccctgg acaccgagag
       61 cgaggcccag gtccagtcgg cgctggagcg cctgatggcc ggccggacca ccatcctgat
      121 cgcccatcgc ctgtcgaccg tgaagggcgc cgaccgcatc tatgtgatcg acaagggcca
      181 ggtggtcgag accgggaccc atgccgaact ggtccgcgcc aagggcctct atgcccgcct
      241 cgccaaggcc caggacctcg atcatctgcc gggcgacgcc gcgtgaggcc gctgcgctcg
      301 cccctggtca tgggcctgct ggtcaatctg atgtcgggct gtctggccct gnnnnnnnnn
      361 ncccgcgagg gccaggcgat cgccgacgat gttcaggccc aggctttggc ggaacgctcg
      421 ggcgtgatcc tggcgctgtg gcactcccgc gtacctgttg gccccgcgac ctggccgcag
      481 ggcgccgaca agccggagat ccgcgtgctg gtgagccagt cgcgcgacgg cgagttcatc
      541 gcccgggtga tcgcccgcct gggcctgccg tccattcgcg gctcgtcgct gaagaagacc
      601 gacaccgcca agaacaaggg tggcgagcag gccttccgcg acatggtcaa gtgggtgaag
      661 gacggcggcg ccatggcgat cacgccggac ggcccgcgcg ggccggtcga ggtattgcag
      721 aagggcgccg tggccctggc gcgggtcagc ggcgcgccgg tgctgttcgt cggcgtggcg
      781 atgaagccct gcattcgcct gggcacgtgg gaccgcacca tcatccccct gccgtttgcc
      841 aaggcggcga tggtctggga cgggccgatc accgccgggc gcgacgacga tcccgacgtg
      901 ctggccgaag cctggggcgc aaggctgtcg gctgtgtcgc gccgggccga gcagatcgtc
      961 ggcgaagacg acggcgcgcc ggtccgccat tgatccgacc cgtccatggt gggatgtagg
     1021 gccatgccgc acgaccattc gggccacgct cacgatcacc atggtcacga ccatggacat
     1081 gaccacgccc acgaccacgg gcatggccat tcgcacggac acggtcacgg ccaccatcat
     1141 cacgtcccca aggacttcgg ccgcgccttc gccatcggca cggcgctgaa cctgggcttt
     1201 gtgctggtcg agttcggggc ggggctgctg acccagtccc tggccctgct ggccgacgcc
     1261 ggccacaatc tgtcggacgt gctgggtctg gttctggcct ggggcgcggc gacgctagcc
     1321 aagcgcgcgc ccagcggccg tcacacctat ggcctgcgca agggcacggt gctggcctcg
     1381 ctgggcaatg ccgccttgct gctggccgcc accggcgcca tcgcctggga ggctatccgc
     1441 cgcttcgccg ccccgacccc ggtcgagagc ggtccggtga tgatcgtggc gaccatcggc
     1501 atcatcatca acactgcgac cgccctgatg ttcatgcgcg gctccaagga cgacctgaac
     1561 gcccgcggcg ccttcctgca catggccgcc gacgccgctg tctcggccgg ggtggtgctc
     1621 gccgccctgc tgatgatgct gaccggctgg ctgtggatcg actcggtgac cagcctgctg
     1681 atcgtggccg tgatcgtgct ggggacctgg ggcctgctgc gcgactcgct ggacatggcc
     1741 ctggacgccg caccgcgcgg catcgacccg gccgccgtcc gcgactggct ggccgcccgt
     1801 cccggcgtca tcgaggtgca tgacctgcac gtctgggcga tgagcacgac ggaaaccgcc
     1861 atgaccgccc acgtcgtgcg tcccacgggc g
//
LOCUS       DAOMEO010000674         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6377_length_1888_cov_2.186034, whole genome shotgun sequence.
ACCESSION   DAOMEO010000674 DAOMEO010000000
VERSION     DAOMEO010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_1888_cov_2.186034"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLH31_13900"
     CDS             complement(<1..783)
                     /locus_tag="PLH31_13900"
                     /inference="COORDINATES: protein motif:HMM:NF018249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HQR90324.1"
                     /translation="MRRLAVVACLLASLMAGPALAAPLEVRVVVVTAFEIGEDTGDKA
                     GESQAWAQVMPEKLPFKAGYRDLRYDPVRKTLLVSTSMGTGRAAISTMALGLDPRFDL
                     SKSYWLVAAIAGVNPNQGSVGSAAWIGAVIDSDFGYAIDPRETPKGWSTGLIPFDRKA
                     PYQTPAPPAPNYNLFPLNTALRDWAFDLTKGSAIADNEALKALRAPYVGLPMAQTPPR
                     VLKGDEVSGQAFWHGEILNRHYEAWANYWTGDQDGFVMTGMED"
     gene            complement(836..1687)
                     /locus_tag="PLH31_13905"
     CDS             complement(836..1687)
                     /locus_tag="PLH31_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQR90325.1"
                     /translation="MIRPQNRLAAIGVAAGALLVLLGSTVYQVDQRQQALVVRFGDPI
                     RVVKSPGLHLKAPFLDQVLKFDNRNIALNADQEEVIAADQERLVVDAFVRYRISDPRQ
                     FYRTLRDETVARDRLERIVNASLRQTIGRANSEEVIAGKRAVVMAAIRDDVARQVAAS
                     NLGVQIIDVRIKRADLPEANQKAVFERMQTARQQEAAQLRAIGEQQKREIVATATEEG
                     EKIRGEGDARRAELFASSFGRDPNFAAFYRSMQAYEASLGQGDATLVLSPDSAFFKYF
                     QRGPQAQ"
     gene            complement(1684..>1888)
                     /locus_tag="PLH31_13910"
     CDS             complement(1684..>1888)
                     /locus_tag="PLH31_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQR90326.1"
                     /translation="NQIYDQYKLAPAVTRERLYIETMQRVLANSNKVIIDSKGASAPV
                     ILPPDAFRPRTATVVKSVDGAVQ"
BASE COUNT          301 a          598 c          657 g          332 t
ORIGIN      
        1 gtcttccatg ccggtcatca caaagccgtc ctggtccccg gtccaataat tggcccaggc
       61 ctcgtagtgc cggttcagga tctcgccgtg ccagaaggcc tggccgctga cctcgtcgcc
      121 cttcagcacc ctcggcggtg tctgggccat cggcaggccg acatagggcg cgcgcaaggc
      181 cttcagcgcc tcattatcgg cgatggccga gcccttggtc aggtcaaagg cccagtcgcg
      241 cagggcggtg ttcagcggaa acaggttgta gttcggcgcg ggcggggcgg gggtctggta
      301 cggcgccttg cgatcgaacg ggatcaggcc ggtgctccag cccttgggcg tctcgcgcgg
      361 atcgatggcg tagccaaagt cgctgtcgat caccgcgccg atccaggcgg ccgagccgac
      421 cgagccctgg ttgggattga ccccggcgat ggccgcgacc agccagtagc ttttcgacag
      481 gtcaaaacgg gggtccaggc ccagggccat ggtcgagatc gccgcgcggc ccgtgcccat
      541 gctggtggag accagcaggg tcttgcgcac cgggtcatag cgcaggtctc gatagccggc
      601 cttgaagggc agtttttcgg gcatgacctg cgcccaggcc tgggattcgc cggccttgtc
      661 gccggtgtcc tcgccgatct cgaaggcggt caccaccacc acccgcacct ccagcggcgc
      721 tgcgagggcg ggacctgcca tcaaactggc gagcaggcag gcgacaaccg cgagacgacg
      781 catgggcttt cccggataga aaaagggccg cgagagaatc tcgcggccct ggttcttact
      841 gcgcttgcgg tccgcgctgg aagtacttga agaaggcgct gtcgggcgac agcaccaggg
      901 tggcgtctcc ctgaccaagc gatgcctcat aggcctgcat cgagcgatag aaggccgcga
      961 agttgggatc ccggccaaag ctggacgcga acagttcggc gcggcgggcg tcgccctcac
     1021 cccggatctt ttcgccttcc tcggtggcgg tagccacgat ctcgcgcttc tgctgctcgc
     1081 cgatcgcgcg aagctgggcg gcttcctgtt gacgggcggt ctgcatgcgc tcgaacaccg
     1141 ccttctggtt ggcttcgggc aggtcggcgc gcttgatccg cacgtcgatg atctggacgc
     1201 ccaggttgga cgcggcgacc tgacgggcga catcgtcgcg aatggcggcc atcaccacgg
     1261 cgcgcttgcc ggcgatcact tcctcggaat tggcgcggcc gatcgtctgg cgcagcgaag
     1321 cgttgacgat gcgctccagc cggtcgcgag ccacggtctc gtcgcgcaag gtgcgataga
     1381 actggcgcgg atcgctgatc cggtagcgga cgaaggcgtc aaccaccagg cgctcctggt
     1441 cggcggcgat cacctcttcc tggtcggcgt tcagggcgat gttgcggttg tcgaacttca
     1501 gcacctgatc caggaacggc gccttcaggt gcaggcctgg cgatttgacc acccggatcg
     1561 ggtcgccgaa gcgaaccacc agggcctgct ggcgctgatc gacctgatag actgtcgagc
     1621 ccagcaggac gagcagggcg ccggcggcga cgccgatggc ggcgagacgg ttttgcgggc
     1681 ggatcattgg acggctccgt caacggactt gacgacggtg gcggttctcg gacggaacgc
     1741 atcggggggc aggatcaccg gagcgctggc gcccttgctg tcgatgatca ccttgttcga
     1801 attggccagc acgcgctgca tggtctcgat gtagagacgc tcgcgcgtga cggccggggc
     1861 cagcttgtac tggtcgtaga tctggttg
//
LOCUS       DAOMEO010000675         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6379_length_1887_cov_2.218886, whole genome shotgun sequence.
ACCESSION   DAOMEO010000675 DAOMEO010000000
VERSION     DAOMEO010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6379_length_1887_cov_2.218886"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLH31_13915"
     CDS             complement(<1..475)
                     /locus_tag="PLH31_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR90327.1"
                     /translation="MVELTLPKNSQVKAGKHWPAPAGATKVKTFKIYRYDPEAGGNPR
                     WDTYDVDMDAVGPMVLDALLYIKNNIDSSLAFRRSCREGVCGSCSMNIGGRNTLACTH
                     GWAEVPGKAVQIAPLPHAPVVKDLIPDLTLFYAQYASIEPWLHADTPEPQTEWKQS"
     gene            complement(483..>1887)
                     /locus_tag="PLH31_13920"
     CDS             complement(483..>1887)
                     /locus_tag="PLH31_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQR90328.1"
                     /translation="YGEGPIQRTCAAADRTGHAMLHTMYGQSLAHDTEFFIEYFALDL
                     IMEDGVCRGITAWKLDDGTLHRFQAQMVILATGGYGRAYFSATSAHTCTGDGNAMALR
                     AGLPLQDMEFVQFHPTGIYGAGCLITEGARGEGGYLTNSEGERFMERYAPSVKDLAPR
                     DMVSRAMTIEIREGRGVGPNKDHIFLHLDHLDPKILHERLPGISETAKVFAGVDVTKA
                     PIPVLPTVHYNMGGIPTNYHGEVVTKSGENPDQVIPGLMAVGEAACVSVHGANRLGSN
                     SLIDLVVFGRAAALRCAEILKPLATQPELKNANTDAHLARFDRYRNASGTQGTAGLRL
                     EMQKAMQEDAAVFRTGETLDGGAQRLANVWEKAKDIKVNDRGLVWNTDLMETLEFDNL
                     IGQAVVTVAGAVNRTESRGAHAREDFGDRNDGEWMKHTLAWLDVDTGKVKIDYRPVHS
                     YTMSDDIAYIPPKARVY"
BASE COUNT          280 a          587 c          663 g          357 t
ORIGIN      
        1 ggctctgctt ccactcggtc tgcggctcgg gcgtgtcggc gtgaagccac ggctcgatcg
       61 aggcgtactg ggcgtagaac agcgtcaggt ccgggatcag gtccttgacc accggcgcgt
      121 gcggcagcgg cgcgatctgc acggccttgc cgggcacttc ggcccagccg tgggtgcagg
      181 ccagggtgtt gcggccgccg atgttcatcg agcaggagcc gcagacccct tcccggcacg
      241 atcggcggaa ggccaggctc gagtcgatgt tgttcttgat atagagcagg gcgtccagga
      301 ccatgggccc gaccgcgtcc atatcgacgt cgtacgtatc ccagcgcgga ttgccgcccg
      361 cctcgggatc gtagcgatag atcttgaagg tcttgacctt cgtggctccg gcgggcgccg
      421 gccagtgctt gccggccttg acctgggagt tcttgggaag cgtcagctcg accatcggac
      481 cctcaataaa cccgtgcttt gggcgggata taggcgatgt cgtcagacat cgtgtagctg
      541 tggaccgggc ggtaatcgat cttcaccttg ccggtgtcga catcgagcca ggccagggtg
      601 tgtttcatcc actcgccgtc gttgcgatcg ccgaagtcct cgcgggcgtg ggcgccgcgg
      661 ctttcggtgc ggttcaccgc cccggccacc gtgaccaccg cctggccgat caggttatcg
      721 aactccaggg tctccatcag gtcggtgttc cacaccagcc cgcggtcatt gaccttgatg
      781 tccttggcct tctcccagac attggccagg cgctgggcgc cgccgtcgag ggtttcgccg
      841 gtgcggaaca cggcggcgtc ttcctgcatg gccttctgca tctcaagacg caggccggcc
      901 gtgccctggg ttccgctggc gttgcgatag cgatcgaagc gggccaggtg ggcgtcggtg
      961 ttggcgttct tcagctctgg ctgggtggcc aggggcttga ggatctcggc gcagcgcagg
     1021 gcggccgcgc ggccgaacac caccaggtcg atcaggctgt tggagcccag acggttggcg
     1081 ccatgcaccg acacgcaggc cgcctcgccc acggccatca ggccggggat cacctggtca
     1141 gggttttcac ccgacttggt gacaacttcg ccgtgatagt tcgtggggat gccgcccata
     1201 ttgtagtgga cggtcggcag gaccggtatc ggggccttgg tcacatcgac gccggcgaac
     1261 accttggcgg tttccgagat gccgggcagg cgctcgtgca ggatcttcgg atccaggtga
     1321 tccaggtgca ggaagatgtg gtccttgttg gggcccacgc cgcggccttc gcggatctcg
     1381 atggtcatgg cccggctgac catgtcgcgc ggggccaggt ccttcacgga cggcgcatag
     1441 cgctccatga agcgctcgcc ttccgaattg gtcaggtagc cgccttcacc gcgggcgccc
     1501 tcggtgatca ggcaaccggc gccatagatg ccggtggggt ggaactgcac gaattccatg
     1561 tcctgcagcg gcaagccggc gcgcagcgcc atggcgttgc cgtcgccggt gcaggtgtgg
     1621 gccgaggtgg ccgagaaata ggcacggccg taaccgccgg tggccaggat caccatctgg
     1681 gcctggaagc ggtgcagggt gccgtcgtcg agcttccagg cggtaatgcc ccggcagacg
     1741 ccatcttcca tgatcaggtc gagggcgaag tactcgatga agaactcggt gtcgtgggcc
     1801 agggactggc cgtacatcgt gtgcagcatg gcgtgaccgg tgcggtcggc cgccgcgcag
     1861 gtgcgctgga tcgggccttc gccgtag
//
LOCUS       DAOMEO010000676         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6383_length_1886_cov_2.741671, whole genome shotgun sequence.
ACCESSION   DAOMEO010000676 DAOMEO010000000
VERSION     DAOMEO010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6383_length_1886_cov_2.741671"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLH31_13925"
     CDS             complement(<1..1006)
                     /locus_tag="PLH31_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HQR90329.1"
                     /translation="MIMNGSEAAKIQPVSKARTEGMKVTERPKLALVRKVQVDRSRDA
                     LLTDFGKTTLEDRYLLPGESYQDMFARVSTAFADDADHAQRVYDYMSKLWFMPSTPVL
                     SNGGAERGLPISCFLNSVSDSLDGILSVWNENVWLAANGGGIGTYWGGVRSIGEKVKG
                     QGQTSGIIPFIRVMDSLTLAISQGSLRRGSAAVYLDIHHPEIEEFLEIRKASGDFNRK
                     SLNLHHGISISDEFMHAVRDGLKFGLRSPKTNEVLREVDARALWQKVLELRLQTGEPY
                     LIFSDTVNRAMPQHQRELGLTVRQSNLCSEIMLHTGVDHLGNDRTAVCCLSSVNAETF
                     M"
     gene            complement(1458..>1886)
                     /locus_tag="PLH31_13930"
     CDS             complement(1458..>1886)
                     /locus_tag="PLH31_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR90330.1"
                     /translation="PPPPPPPPPPPPPPPPPPPPPPPPPPPPPAYEAREFIVYFPFDQ
                     YVLTPEAQSVVSEAANYANAGKATKLVIVGHTDTSGSPKYNARLSERRAKAVADALVG
                     MGVATSTVAVDWKGESAPAVATGDGVKEPLNRRSTISINF"
BASE COUNT          327 a          597 c          630 g          332 t
ORIGIN      
        1 ccatgaaggt ctcggcgttg acgctcgaca ggcagcagac ggccgtgcgg tcattgccca
       61 ggtggtcgac accggtgtgc agcatgatct cgctgcacag attggactgg cgcaccgtca
      121 ggcccagctc gcgctggtgc tgcggcatgg cgcggtttac ggtgtcggag aagatcagat
      181 agggctcgcc ggtctgcagg cgcagctcca gcaccttctg ccacagggcg cgggcgtcga
      241 cctcgcgcag cacttcgttg gtcttcggcg agcgcaggcc aaacttcagg ccgtcgcgga
      301 cggcgtgcat gaactcgtcg ctgatcgaaa tgccgtggtg caggttcagg gacttgcggt
      361 tgaagtcgcc tgacgccttg cggatttcca ggaactcttc gatttccggg tggtggatgt
      421 ccagatagac ggcggccgat ccgcgacgca ggctgccctg cgagatggcc agggtcaggc
      481 tgtccatcac gcggatgaag gggatgatgc cgctggtctg gccctggccc ttgaccttct
      541 cgccgatcga ccggacgccg ccccaatagg tgccgatgcc gccgccattg gccgccagcc
      601 agacgttctc gttccagacg ctgaggatgc cgtccaggct gtcggagacc gagttcagga
      661 agcagctgat cggcagaccg cgctcggcgc cgccgttgct gagcaccggc gtcgacggca
      721 tgaaccacag cttgctcatg tagtcataga cgcgctgggc gtggtcggcg tcgtcggcga
      781 aggccgtcga cacgcgggcg aacatgtcct ggtaggactc gcccggcagc agatagcggt
      841 cttccagcgt ggtcttgccg aaatcggtca gcagggcgtc gcgcgagcga tccacctgca
      901 ccttgcgcac caaggccagc ttgggacgct cggtcacctt catcccctcg gtacgcgcct
      961 tggatacggg ctgaatcttc gccgcttcac tgccgttcat gatcaaaatc cataatcaat
     1021 cagcgtgttc cggtgaaggg aacgccccgc ccagccgcta catcttgtgg ccgaggagcc
     1081 ccctcagccc acatatgggg ccacagtggc taatgactcg tcaacgcccc gagcgcgagt
     1141 ttttcgttaa caagctgtga acggcgtgaa cttaccgggc aggatcaagg ggttgcccat
     1201 accaactttt ttcacctgcg acagaccgtc acaaggtgcg ccaaccaatc cgcccccgga
     1261 cttcgacaag cacccacagc cgcgtctgca tgagcttctg cggggccgcc tggcgcatcg
     1321 gaaagtgtct gcgcgcgcct caagcgcagg gcgggactta agaccccttg ccgccgctgt
     1381 ctgcgttggc tcacgatcca aaagaaaagg gcctggcgtc gccgccaggc ccccctcaga
     1441 tcgcgttgat ccagatctta gaagttgatc gagatcgtag agcggcggtt cagcggttcc
     1501 ttcacaccat cgcccgtggc gacggccgga gcgctttcgc ccttccagtc aacagccacg
     1561 gtcgaggttg caacacccat gccgaccagc gcgtcagcaa cggcctttgc acgacgctcc
     1621 gacaggcggg cgttgtactt gggcgagccc gacgtgtcgg tgtgaccgac gatgaccagc
     1681 ttggtcgcct tgccagcgtt ggcgtagttc gcggcttccg aaaccaccga ctgggcttcc
     1741 ggcgtcagca cgtactgatc gaacgggaag tagacgatga actcgcgagc ctcatacgcc
     1801 ggcggaggcg gcgggggagg cggcgggggc ggaggcggcg gcgggggcgg cggcgggggc
     1861 ggcgggggcg gcggaggcgg cggggg
//
LOCUS       DAOMEO010000677         1886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6384_length_1886_cov_2.313490, whole genome shotgun sequence.
ACCESSION   DAOMEO010000677 DAOMEO010000000
VERSION     DAOMEO010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6384_length_1886_cov_2.313490"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLH31_13935"
     CDS             <1..729
                     /locus_tag="PLH31_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR90331.1"
                     /translation="SAPTLHVTLTATGLDIDITGVEAKSGGLSADARMVAAMAASEGD
                     FARVTLAGEVVYMARQPLVKLGPAVVALSPGSFLQAVPQAEKAMVAFAVEAAQGATKI
                     ADLYCGAGTFTFRLAEVAAVHAADSSAPAISALKAAIGGAPGLKPITAEARDLVRRPV
                     LTMELAKTDVVVIDPPRAGALEQTVEIANSKVARVISVSCNPATFARDARMLVDAGFK
                     LEKVLPVDQFVWSPHIELVGVFSR"
     gene            737..1789
                     /locus_tag="PLH31_13940"
     CDS             737..1789
                     /locus_tag="PLH31_13940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR90332.1"
                     /translation="MDQSPGRNAFALDAVGQRARAATKAAWWSAAGGLARALTRPTQG
                     QAARTFEPSGPPVSGARLRQVYLEAFEKDARDVAAGLYPAMEDAPTRPMKSLLQALDF
                     LVDAKAVDQRRRRGDGVEVRSEPETQAYPNYYRQNFHYQSGGWFTAESARRYDAQVEA
                     LFAGTAGAMRRRGLSLLARHWRGRDQRNLRMVDAACGSGAFLRDLKAAFPRAAMAGVD
                     LSSAYLAKARRRAGVGAVQANVETLPFADGALDAVSCVYLFHELPPRVRPVVAAAMAR
                     VLKPGGVLVFVDSVQPTDEPHLERLLEAFPAYFHEPYYSTYAQTDLAALFRGAGLRLV
                     SEDRAFLTKAMLFEKA"
BASE COUNT          279 a          640 c          661 g          306 t
ORIGIN      
        1 tcggccccga ccctgcacgt caccctgacc gcgaccggtc tcgatatcga catcaccggc
       61 gtcgaagcca agagcggggg cctgtcggcc gacgcccgga tggtcgccgc catggcggcc
      121 agcgaaggcg acttcgcgcg cgtcactctg gccggcgagg tcgtctatat ggcccgccag
      181 ccgctggtga agctggggcc ggccgtggtc gccctgtcgc cgggctcctt cctgcaggct
      241 gtgccccagg ctgaaaaggc catggtcgcc ttcgctgtcg aggccgccca gggcgccacc
      301 aagattgctg atctctactg cggggcaggg acgttcacct tccgcctggc ggaagttgcg
      361 gcggttcatg cagcggactc ctcagcgccg gccatcagcg ccctgaaggc cgcgatcggc
      421 ggcgcgccgg gcctcaagcc gatcaccgcc gaggcgcgcg atctggttcg tcggccggtg
      481 ctgaccatgg aactggccaa gaccgacgtg gtcgtcatcg acccgccgcg cgccggggcg
      541 ctggaacaga ccgtcgagat cgccaactcc aaggtcgccc gggtgatcag cgtctcgtgc
      601 aaccccgcca ccttcgcccg cgacgcccgg atgctggtcg acgccgggtt caagctggaa
      661 aaggtgctgc cagtagacca gttcgtctgg tcgccccata ttgaactcgt tggggtcttc
      721 tcgcgatgag gcaggcatgg atcagtcacc cgggcggaac gcgttcgcgc tagacgctgt
      781 tggacagcgc gccagggcgg cgaccaaggc cgcctggtgg agcgcagcag gcggccttgc
      841 gcgagccctc acaaggccaa cccaggggca agccgcaagg accttcgagc ccagtgggcc
      901 acccgtctca ggggcgcggc tgcggcaggt ctatctcgag gccttcgaga aggacgcgcg
      961 cgacgtcgcc gccggcctct atcccgccat ggaagacgcg ccgacgcggc caatgaagtc
     1021 gctgttgcaa gccctggatt tcctcgtgga cgccaaggcc gtcgaccaga ggcgccggcg
     1081 aggggacggt gtcgaggtaa ggtccgagcc tgaaacccaa gcctatccaa actattaccg
     1141 gcagaatttc cactaccagt ctggtggctg gttcaccgcc gagagcgccc ggcgctatga
     1201 cgcccaggtc gaggcgctgt tcgccggcac ggcaggcgca atgcgtcggc gcgggctgtc
     1261 gctgctggcc cgtcattggc ggggtcgcga tcagcggaac ctgcggatgg tcgacgcggc
     1321 ctgcggctcg ggcgccttcc tgcgcgacct gaaggccgcc tttccccgcg ccgccatggc
     1381 gggcgtcgat ctgtcatcgg cctatctggc caaggcccgg cgccgggcag gggttggcgc
     1441 tgtgcaggcc aatgtcgaga ccctgccgtt cgccgatggc gcgctggacg cggtcagctg
     1501 cgtctatctg ttccatgagt tgccaccgcg cgttcggccg gttgtcgcgg ccgccatggc
     1561 tcgggtgttg aagcccggcg gcgtgctggt cttcgtcgac tcggtgcagc cgacagacga
     1621 gccgcacctg gagcggctgc ttgaggcttt cccggcctat ttccacgagc cctactattc
     1681 gacctacgcc caaaccgatc tggcggccct gttccgcggc gctggcctgc ggttggtcag
     1741 cgaggaccgg gcgttcctga ccaaggcgat gctgttcgag aaagcctgag gctggacggg
     1801 cgggcgcggg cgcggcacta agcggccctg tgtcgggggc aagcgcgttg gggcaatcga
     1861 tcgacggcgg gcgtcgctat gaggcc
//
LOCUS       DAOMEO010000678         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6417_length_1880_cov_2.408219, whole genome shotgun sequence.
ACCESSION   DAOMEO010000678 DAOMEO010000000
VERSION     DAOMEO010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_1880_cov_2.408219"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLH31_13945"
     CDS             <1..1121
                     /locus_tag="PLH31_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90333.1"
                     /translation="LANMSHEIRTPMNGVLGVLHLLKQEDLTDDGRQLLNEALGCGAM
                     LSELLNDIVDFSKVEAGKLELAPEPTDPAALLRGVADLLRPQTRQRGLYLRVETPPDL
                     GWVSVDPVRLRQALFNLLGNAAKFTLEGGIDIRLTARPSERGLSLRFEFQDTGIGIAE
                     EAGRHLFERFSQADGSTTRRFGGAGLGLAITRRLAHLMGGEIGFTSTLGQGSIFWLEI
                     YAERADAIQTREEADRPLFGGRRFLLVEDNATNRMVASRMLEAMGASVECAEDGAHGV
                     EAARRGFDLILMDIQMPGMDGVEATRRIRALGGEIGATPILAMTANALAHQQASYLAA
                     GMNGAVAKPLSPAALIQTVAAALDSAEDAQQSSVLQQS"
     gene            1254..1880
                     /locus_tag="PLH31_13950"
                     /pseudo
     CDS             1254..1880
                     /locus_tag="PLH31_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676192.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          311 a          650 c          611 g          308 t
ORIGIN      
        1 tcctggccaa tatgagccac gagattcgca cgcccatgaa cggcgtgctg ggcgttctgc
       61 atctgctcaa gcaggaggac ctgaccgacg acggccgtca gctgctcaac gaggccctgg
      121 gctgcggggc catgctgtcg gaactgctca acgatattgt cgatttttcc aaggtcgaag
      181 cgggcaagct ggaactggcc cccgagccga ccgatccggc cgccctgctg cgcggcgtcg
      241 ccgatctgct acgcccgcag acacggcagc gggggctgta tctgcgcgtt gagacccccc
      301 cggatctggg ctgggtgtcg gtcgatccgg tacgtctgcg tcaggcgctt ttcaatctgc
      361 tgggcaatgc cgcaaagttc accctggaag gcggcatcga tatccgcctg accgcccggc
      421 catcggagcg gggtctgagc ctgcgcttcg agttccagga caccggcatc ggcatcgccg
      481 aggaggctgg ccgtcatctg ttcgagcgct tcagccaggc cgacggctcg accacccgcc
      541 gcttcggcgg cgcgggtctt ggcctggcca tcacccgccg gctcgcccat ttgatgggcg
      601 gcgagatcgg cttcaccagc accctgggcc agggctcgat cttctggctc gagatctacg
      661 ccgaacgggc tgacgccatc cagacccgcg aagaggccga ccgtccgctg ttcggcggcc
      721 gacgcttcct gctggtcgag gacaacgcca ccaatcgcat ggtcgcctca cgcatgctgg
      781 aggcgatggg cgccagcgtc gaatgcgccg aggacggagc ccacggcgtc gaggcggcgc
      841 gccgcggctt cgacctgatc ctgatggata tccagatgcc cggcatggat ggcgtcgagg
      901 ccacccgacg catccgggcg ctgggcggag agatcggcgc gaccccgatc ctggccatga
      961 ccgccaacgc cctcgctcac cagcaggcga gctatctggc ggccggaatg aacggcgctg
     1021 tcgccaagcc gctctccccc gccgccctga tccagaccgt ggccgcggcc ctggacagcg
     1081 ccgaagacgc ccaacagagc agcgttctgc aacaaagctg aaaaaccccg cgggcgccgc
     1141 cctgttattg tcgccaaaat cgggaagcgg tctttgcgtc gctccccgcc acgcctaact
     1201 tggactgtac agtccagcag ccggcgtaga tggtgcttgg gggatcctgc cttatggatg
     1261 acggacggat ctcgggcgtg gccacggcgc gctatgcgcg caagaaggaa gccatcctgg
     1321 tcagggcgtg cgcggcatga ccctggccga cgtcgcggct cgcgtgggcc tgatcaccac
     1381 cagcgtcacc tactattacc gcaagaagga cgatctggcg gccgcctgct tcatgcgcgg
     1441 tctggagcgt ttcgacgcga tgatcgccca ggcggcggag cagcccgacc cgccgtcgcg
     1501 cctgaagacc ttcatcgacc tctatctgga tctgaaccgc cgcgtccggc tgggcgaagc
     1561 cacgcccctg accagcttca ccgagatcaa ggccctgaag gagcccctgc gctcgtcggt
     1621 ttccaacgcc ttcaacgatc tgttccggcg ggtgcgcggc ttcttcgagg gcgagggctt
     1681 cgagcatctg gaaaagcgcg aacgcaacgc ccgcacccac ctgctgatgg agcaggtgtt
     1741 ctggtcgggc acctggctgc ggcgctacga tctggacgac tacgaacgcg ttcgcgacaa
     1801 gatctatgac attctggtcg gcgggctggc gggccctgga caggtctggg cgcccgccgc
     1861 cctgcccgac cccaccccgg
//
LOCUS       DAOMEO010000679         1875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6433_length_1875_cov_3.076374, whole genome shotgun sequence.
ACCESSION   DAOMEO010000679 DAOMEO010000000
VERSION     DAOMEO010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6433_length_1875_cov_3.076374"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLH31_13955"
     CDS             complement(<1..485)
                     /locus_tag="PLH31_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90334.1"
                     /translation="MRALALLFALIAAPALAQEAPVTTAPSPVAQQANPAQPAAEPAA
                     EPPAAEPYVPPAIDPLGDLISQTTDEEEEAAPSSRPAPHKPTLLPIPAPESPAPSIAA
                     GEHLVPQGSYMPAEQIYEMRVKGSIVAAQGLQGPLDGGWKVKGPDGSTLYTLQIVDPA
                     GG"
     gene            complement(494..1369)
                     /locus_tag="PLH31_13960"
     CDS             complement(494..1369)
                     /locus_tag="PLH31_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HQR90335.1"
                     /translation="MSDVARPRRSALYMPASNAKAVEKARTLDADVIILDLEDAVAPE
                     MKEAARDAAVAAVKAGGFGSREIVIRTNGLDTPWGAADLKAAAEAGPDAVLVPKVNDA
                     ADVHAYDAALHAAPIHTRLWTMIETGKAAFHLWEIAAASKSTRLSAWVMGVNDFAKEM
                     RARQTPDRAPFLPLLTLSVAAARAHGLTILDGVHNDIEDLEALAAVCVQGVDFGFDGK
                     TLIHPKHLEICNRVFSPTEGEIAWSQAVIAAFKAPENAGKGALRVDGKMAERLHLTQA
                     ERLVQVAAAIAAKAG"
     gene            1498..>1875
                     /locus_tag="PLH31_13965"
     CDS             1498..>1875
                     /locus_tag="PLH31_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HQR90336.1"
                     /translation="MTTRYDENARNVLGGELAPCSLNPVTGFYRNGCCETGPHDLGLH
                     TVCAVMTEAFLAFSRAAGNDLSTPRPEYGFRGLKAGDRWCLCAGRWKEALDAGLAPPV
                     VLEASHEEMLAIVPLGVLKDHAAV"
BASE COUNT          277 a          612 c          674 g          312 t
ORIGIN      
        1 ccgccggccg gatcaacgat ctgcagcgtg tagagggtcg atccgtcagg gcccttgacc
       61 ttccagccgc catccagcgg gccttgcagg ccctgcgccg cgacgatcga gcccttgacc
      121 cgcatctcgt agatctgttc ggcgggcata tagctgccct gcggaaccag atgctcgccc
      181 gccgcgatgg acggcgccgg cgattccggg gccgggatcg gcagcagggt cggcttatgt
      241 ggcgccggac gcgacgaggg cgcggcctcc tcttcctcat ccgtggtctg cgagatcagg
      301 tcgcccagcg gatcaatggc ggggggcaca tagggttcag cggcgggggg ttcagcggcg
      361 ggctccgcag cgggctgggc ggggttcgcc tgctgcgcga cgggcgacgg cgcggtcgtc
      421 accggcgcct cctgggccag ggccggggcc gcgatcagcg cgaaaaggag agccagagcc
      481 cgcatgaaac gtcctaaccg gccttggcgg cgatggcggc ggccacctgg accaggcgct
      541 cggcctgggt caggtgcagg cgctcggcca tcttgccgtc gaccctgagc gctcccttgc
      601 ccgcattttc gggcgccttg aaggcggcga tcaccgcctg gctccaggcg atctcgccct
      661 cggtcggcga gaacacccgg ttgcagatct ccagatgctt aggatggatc agggtcttgc
      721 cgtcaaagcc gaaatccacg ccctggacgc agaccgccgc aagcgcttcg aggtcctcga
      781 tgtcgttgtg cacgccatcc aggatggtca ggccgtgggc ccgggcggcg gcgaccgaca
      841 gggtcagcag cggcaggaac ggcgcgcggt ccggcgtctg gcgggcccgc atctccttgg
      901 cgaagtcgtt gacgcccatc acccaggccg acagccgggt cgacttgctg gcggcggcga
      961 tctcccacag gtggaaggcg gccttgccgg tctcgatcat ggtccacagc cgggtatgga
     1021 tcggggcggc gtgcagggcg gcgtcatagg cgtggacatc ggcggcgtca ttgaccttgg
     1081 gcaccagcac cgcgtcgggc ccggcctcgg cggcggcctt cagatcggcc gcgccccagg
     1141 gggtgtcgag gccattggtg cggatgacga tctcgcgcga tccaaagccg ccagccttca
     1201 cggcggcgac ggcggcgtca cgggcggcct ctttcatttc cggggccacg gcgtcttcaa
     1261 ggtcaagaat gatcacgtcg gcgtcgaggg tgcgcgcctt ctccaccgcc ttggcgttgg
     1321 aagcgggcat gtagagggcg ctgcggcgcg gccgggcgac gtcggacatt caaaattccc
     1381 tgcgttgctt gttcttatac agccttctta atcacgcgcg ggccccggca aaagcaaaga
     1441 cgttcgtcgc gcgctaatgt tggtcgagga tcgcgcaaag cgggttagga tgccggcatg
     1501 accacgcgct atgacgagaa cgcccgcaac gttctgggcg gcgaactggc cccctgttcc
     1561 ctgaacccgg tgacgggctt ctaccgcaac ggctgttgcg aaaccgggcc gcacgatctg
     1621 ggcctccata ccgtctgcgc ggtgatgacc gaggcgttcc tggccttctc cagggccgcc
     1681 ggcaatgatc tctcgacgcc ccgtcccgaa tatggctttc gcggcctgaa ggcgggcgat
     1741 cgctggtgtc tgtgcgccgg gcgctggaaa gaggccctgg acgcgggcct ggccccgccg
     1801 gtggtgctgg aagcctcgca tgaggaaatg ctggcgattg tgccgctggg ggtgctgaag
     1861 gaccacgcgg cggtt
//
LOCUS       DAOMEO010000680         1872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6445_length_1872_cov_2.260870, whole genome shotgun sequence.
ACCESSION   DAOMEO010000680 DAOMEO010000000
VERSION     DAOMEO010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_1872_cov_2.260870"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1134)
                     /locus_tag="PLH31_13970"
     CDS             complement(43..1134)
                     /locus_tag="PLH31_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90337.1"
                     /translation="MPLTAELTATAPRTPDGKPYNCYAIDTTAGGRWTVTLKSQAFDS
                     GLSIARGALCSAASLQHQNDNAAPGDLDARVTFTAVGGRYLILARGMSTTAAGAYELT
                     ATPEAADLGYGGSTQLADNRTDARRALMEAQVGERRAQLAEEAARKQAAEQAATQARL
                     NAEAAARRAERERKRARSEAFNSFLGAAVGAMGEVTNQMAAENSRIQAETSAMIARQA
                     AQAEVQRAAQQRAQAQARAQAAEQAARQLAQNKALVAASRNGGGGGYGIGYSPQTRAA
                     AAQQQNAAQVQSGAAASQVLAQQQAQQRQLAEQQRLAQQRAAEQQASQQRFAQQQAAQ
                     ARAEADRQQRAQEAERRRMAQQQADGLLT"
     gene            1305..>1872
                     /locus_tag="PLH31_13975"
     CDS             1305..>1872
                     /locus_tag="PLH31_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90338.1"
                     /translation="MDVWQNAGIPICGVFAWIRVGGCAIPPAVLQVRRVSDRMTLETE
                     SAEDVWLRGHDAQVSSIQTAGFPPQNGALFSGYALARTQRLGGVVLDRSGGLLFANSA
                     FSALFGKTPPNVEAALEALTQRRSRMAFSTNDKRLAAIVYAPADQGLTWSLPPEVRQE
                     AQRPDAAILGLAVAGIDGGKALDDACLAL"
BASE COUNT          273 a          618 c          631 g          350 t
ORIGIN      
        1 catccatatt ggctctacca aatgccatca tgccgtgtgc ttttaagtta gtaatccgtc
       61 agcctgctgc tgcgccatgc ggcgacgctc ggcctcctga gcgcgctgct ggcgatcagc
      121 ctcggcccga gcctgggcag cctgttgctg ggcgaaccgt tgctgggagg cctgctgctc
      181 ggccgcgcgt tgctgggcca gacgctgttg ttcggcgagc tgtctttgct gggcctgttg
      241 ttgagccagg acctgtgacg ccgcggcgcc ggactgaacc tgggccgcgt tctgctgctg
      301 agccgccgca gcgcgggtct gggggctata gccaatgcca tagcctcccc cgccaccgtt
      361 gcgcgatgcg gcgacgaggg ccttgttctg ggcgagttga cgcgcggcct gctcggcggc
      421 ctgcgccctg gcctgggcct gggcgcgctg ctgggccgcg cgttgaacct cggcttgggc
      481 ggcctggcgg gcaatcatcg cggaggtttc cgcctggatg cgactgttct cggccgccat
      541 ctggttggtg acctcgccca tagcgcccac cgccgcgccc aggaaactgt tgaaggcctc
      601 ggatcgggct cgtttgcgct cacgctcggc gcgccgcgcg gcggcttcgg cgttcaacct
      661 ggcctgggta gccgcctgct cagccgcctg tttccgcgcc gcttcctcgg ccagttgagc
      721 gcgccgctcg ccgacctggg cctccatcaa ggcccggcgc gcgtccgtcc tgttgtcggc
      781 gagttgtgtc gagccgccat agccgagatc agcggcctcc ggtgtggcgg tgagctcata
      841 agcgcccgcc gccgtggtcg acattccacg ggccaggatc agataccgtc cgccgacggc
      901 ggtgaaggtc acccgcgcat ccaggtcgcc cggcgccgcg ttgtcattct gatgctgaag
      961 agacgcggcc gaacaaagag cgccccgcgc gatggatagg ccgctgtcaa acgcctgcga
     1021 cttcagggtg acggtccagc gacctccagc ggtcgtgtcg atggcgtagc agttgtaggg
     1081 tttgccatcc ggcgttcggg gcgcggtggc ggtcagctcc gccgttagag gcaagccaac
     1141 ctccaatgac gcgggcctga ccaagcctgg cgacgaggtc cccggcgtga tctgggccca
     1201 acccagactt gggatggcga aagccgccgc cacgcaacgc caaaagtatg gtttgaccat
     1261 gagcgacgcc gaaccccgaa ccatgacgct tacaaagcat caccttggac gtttggcaaa
     1321 acgcgggtat ccccatttgc ggggtatttg cgtggatccg cgttggagga tgcgcgatcc
     1381 cgcccgctgt attgcaggta agacgcgtat cagaccgcat gacacttgag accgaaagcg
     1441 ccgaggacgt ctggttgaga gggcatgatg ctcaggtctc gtcgatccag accgctggct
     1501 tcccgcccca gaacggcgcg cttttctcgg gctatgccct agcgcgaacc cagcggcttg
     1561 gcggtgtggt cttggaccgg tccggcgggc tgctgttcgc aaattccgcc ttcagcgctc
     1621 tcttcgggaa gacaccgccc aacgtcgagg cggccctgga ggccttgact cagcggaggt
     1681 cccggatggc cttttcgacc aatgacaagc ggctagccgc gatcgtctac gcccctgcgg
     1741 atcagggcct gacctggtcg ttaccgccgg aggttcgaca ggaggcgcag cggcctgacg
     1801 ccgccatcct agggctggcc gtcgcaggca tcgacggtgg caaggcgctg gacgatgctt
     1861 gcctggcctt gg
//
LOCUS       DAOMEO010000681         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6470_length_1866_cov_1.908338, whole genome shotgun sequence.
ACCESSION   DAOMEO010000681 DAOMEO010000000
VERSION     DAOMEO010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_1866_cov_1.908338"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="PLH31_13980"
     CDS             complement(<1..909)
                     /locus_tag="PLH31_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR90339.1"
                     /translation="MTFDIDAIANRRRLLAGLGGLGLMGAAGCAPGQLARPEPMAGLG
                     DFDTLPRLAPIRMRPDRISKITVCLRPFRKAGPRLDVETVGDKTVVHNYGHGGSGWSL
                     SWGSGTIAAKNALSTGERDIAVIGCGALGLTTATLLRRAGANVTIYASERTPDTRSAR
                     ATGTWSPDSRIAAVGATAPGFEAQWEQMCRTSYAAFQTYLGLAGDPVEWTDRYSLFDN
                     PPGPGRATPHDPNGVRFVEYVDRVRDITPRSLDLAPGSHPFPTPYARRGTAMQFNIAE
                     YAHLLMTDYLLAGGKIETMTFHTPADL"
     gene            complement(1042..1413)
                     /locus_tag="PLH31_13985"
     CDS             complement(1042..1413)
                     /locus_tag="PLH31_13985"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR90340.1"
                     /translation="MSSKRRLDLTLQARDDIAELLDTSAERFGQISRRRYEALVARVL
                     EDVRVDPRRPGSLARPELGPGTRTYHFRFSRDRGRTDSGAVKSPRHLLVYETPDEHAV
                     LVLRVLHDAMDIEQHVGADSQ"
     gene            complement(1410..1688)
                     /locus_tag="PLH31_13990"
     CDS             complement(1410..1688)
                     /locus_tag="PLH31_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HQR90341.1"
                     /translation="MAARNVNLTDHLANFVDANVATGRFQNASEVVREGLRLLEQRQR
                     EDDLKLERLRQAVELGREDARHGRTLRIEPGEVDAFLAGLGRTGRPTR"
     gene            complement(1745..>1866)
                     /locus_tag="PLH31_13995"
     CDS             complement(1745..>1866)
                     /locus_tag="PLH31_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90342.1"
                     /translation="LVQAVAASHGGRLELAEGPGEYNGMGPGLRVALVLPRVE"
BASE COUNT          305 a          643 c          616 g          302 t
ORIGIN      
        1 cagatcggcg ggcgtgtgaa aggtcatggt ctcgatcttg ccgccggcca gaagatagtc
       61 ggtcatcagc aggtgggcat attcggcgat gttgaactgc atcgcggttc cgcgtcgcgc
      121 atagggcgtc gggaacggat ggctgcccgg cgccaggtcc aggctgcgtg gcgtgatgtc
      181 ccgcacccga tcgacatact ccacaaaccg cacgccgttc ggatcgtgcg gcgtggcgcg
      241 ccccgggccc ggagggttgt cgaacagact gtagcggtcg gtccattcca ccggatcacc
      301 ggccaggccc agataggtct ggaaggctgc ataggaggtg cggcacatct gctcccactg
      361 cgcttcgaag ccgggggcgg tcgcccccac ggcggcgatg cgggagtcgg gcgaccaggt
      421 gccggtggcg cgggccgagc gcgtgtcggg cgtgcgctcg ctggcataga tggtgacatt
      481 ggcgccggcc cggcgcagca gcgtcgcggt cgtcaggccc agagccccgc agccgatcac
      541 cgcgatatcg cgctcgccgg tcgacagcgc gttcttggcc gcgatggtcc ccgagcccca
      601 cgacagcgac cagccgctgc cgccatggcc gtagttatgg acgacggtct tgtcgccgac
      661 ggtttcgaca tccagacgcg gcccggcctt gcgaaacgga cgcagacaga cggtgatctt
      721 ggagatccgg tcgggcctca tgcggatcgg cgccaggcgc ggcagggtgt cgaagtcacc
      781 cagacccgcc atcggctcag ggcgagcgag ctggcccggc gcgcagccgg ccgcgcccat
      841 caggccaagc ccgccgagcc cggccagaag ccggcgccgg ttagcgatcg cgtcgatgtc
      901 gaaagtcacg ccgggtcctc gcctccgtgg aacacagcgc caccctggac cagcctcgtc
      961 cggggttcga gccagaaccg acgaccgtcc acagatgtgc gcgccccgcc gccgaaaccg
     1021 gcgccgtgcc ctgcgagtag gctattggct gtcagcgcca acgtgctgtt cgatatccat
     1081 cgcatcgtgc aggacacgca acaccaggac agcgtgctcg tctggcgtct cgtagaccag
     1141 cagatgccgc gggcttttga cggccccgct atcggtgcgg cctcgatccc gactgaagcg
     1201 aaagtggtag gtacgcgtcc ccgggcccaa ctctggacgc gccaggctgc ccggtctccg
     1261 aggatcgacg cggacatcct ccaggacgcg ggccaccagc gcttcatagc gtcggcggct
     1321 gatctgtccg aaacgttccg cactggtgtc gaggagttcg gcaatgtcgt cgcgcgcttg
     1381 aagcgtcaga tccagccggc gcttgctgct cacctcgtgg ggcgtcccgt acgtcctagt
     1441 cccgccagaa acgcgtccac ttcgccgggc tcgatgcgca aggtgcgacc gtgtcgggcg
     1501 tcttcgcgac ccagttcgac cgcttgccga aggcgctcaa gcttgaggtc atcttcgcgc
     1561 tggcgttgct ccagcagcct caagccctcg cggaccactt cgctggcatt ctgaaaccgc
     1621 cccgtggcga cattcgcatc gacaaaattc gccagatggt cggtcaaatt cacattgcgc
     1681 gccgccatgg ctcaccctcc gaacgtggtg ataagcctag gatcgttgtc aatctttgac
     1741 aagtttactc gacccgggga agcaccaagg cgacccgcag gcccggcccc atgccgttgt
     1801 actcacccgg cccctcggcc agttccagcc gcccgccgtg ggaggcggcg accgcctgga
     1861 ccagcg
//
LOCUS       DAOMEO010000682         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6478_length_1863_cov_3.052544, whole genome shotgun sequence.
ACCESSION   DAOMEO010000682 DAOMEO010000000
VERSION     DAOMEO010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6478_length_1863_cov_3.052544"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLH31_14000"
     CDS             complement(<1..1010)
                     /locus_tag="PLH31_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR90343.1"
                     /translation="MSRSSSPRARLAVAAAAIALAAGLAGYGLARLQPGRAPTPAAKE
                     QSRVLYWYDPMVPAQHFDKPGKSPFMDMQLVPKYAGEGGAAAPGVVIDPAKLQNLGVR
                     YATAEGGQLDSSLTATGVLEFNERDIAIVQAKASGFVQRVYGRAPGDVVGAGAPLADV
                     LVPEWAGAQAEFLAVRRSGDTALASAARQRLILLGMPPAAVAEVERSGRPRSVLTIAT
                     PSGGVIKTLNVRSGMTVSAGQTLAEVNGVGSVWLNAAVPEALAGQVRPGQPVVATLAA
                     YPTETFNGRVAAILPQANSETRTLTMRVELANPVGRLRPGMFASVRFDAAPQPALLVP
                     SEA"
     gene            complement(1007..>1863)
                     /locus_tag="PLH31_14005"
     CDS             complement(1007..>1863)
                     /locus_tag="PLH31_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR90344.1"
                     /translation="EVRTATALAWINLYYAERRQAAVGEVLTRLEPLWSAAPSAVASG
                     AARPAQSLEAEQMRADLEDRRSEVTATVGRARAELSRWTGDPDPEAAGSPPSFEVNGP
                     ALRAALEGHPRLEEQTAMAAQAQADVRLAQAGKRPDWSWEVAYQRRDPMFGDMVSAGV
                     TVTLPLFGAGRQDPIIAAKVAAAGQARAEREVARRDLVALLDAALADHIMHHDQWSRS
                     QSVLLPLAQKRADLETASYGAGRAGLSDVLQAFTTLANTQLTLLDREAAVAADAASLV
                     LTFGNDQP"
BASE COUNT          247 a          659 c          693 g          264 t
ORIGIN      
        1 gcttccgacg ggacgagcag ggcgggctgt ggcgccgcgt caaaccgcac gctggcgaac
       61 atgccgggac gcaagcgccc cacggggttg gccagttcga cgcgcatcgt cagggtccgg
      121 gtctcgctgt tggcctgcgg caaaatggcg gcgacccgcc cgttaaaggt ctccgtcggg
      181 taggcggcca gcgtcgcgac gaccggctgg ccagggcgga cctggcccgc gagcgcctcc
      241 ggaacggcgg cgttcagcca gacgctcccg acgccgttca cctccgccag ggtctggccg
      301 gcgctcaccg tcatcccact gcggacgttg agggtcttga tcacgccccc agacggggtc
      361 gcgatggtca ggacgctgcg gggtcggccg ctgcgctcca cctccgccac ggccgccggc
      421 ggcatgccga gcaggatcag gcgctggcgg gcggcgctcg cgagggcggt atcgccgctt
      481 cggcgcaccg ccaggaactc cgcctgggcg cccgcccact cggggaccag cacgtcggcg
      541 agcggcgcgc cggcgccgac cacatcgcct ggcgcgcggc cgtagacgcg ctggacgaac
      601 cccgacgcct tggcctggac gatggcgatg tcgcgctcgt taaactccag cacccccgtg
      661 gcggtgaggc tgctgtcgag ctggccacct tcggccgtgg cgtagcggac gccgagattt
      721 tgcagcttcg ccgggtcgat gaccacgccg ggcgcagcgg cgcctccctc gccggcgtat
      781 ttcgggacca gctgcatgtc catgaagggc gacttgcccg gcttgtcgaa gtgctgagcc
      841 gggaccatcg gatcgtacca atagagcacc cggctctgct ccttggcggc cggagtcggg
      901 gcgcggcctg gctgcaaccg cgccaggccg tagccggcca acccggcggc cagggcgatc
      961 gcggcggcgg cgacggccag ccgcgcgcgg ggtgaggacg aacggctcat ggctggtcat
     1021 tcccaaaggt gagcaccagg cttgcggcgt cggcggcgac ggcggcttcc cggtcgagaa
     1081 gggtgagttg agtgttggcg agcgtcgtga aggcctgcag cacgtcgctg aggccggctc
     1141 ggccggcgcc gtagctggcg gtctcgagat cggcgcgctt ctgcgccagc ggcagcagga
     1201 ccgattgcga gcggctccac tgatcgtggt gcatgatgtg atcggccagc gccgcatcga
     1261 gcagggcgac gaggtcgcgc cgggcgacct cgcgctcggc gcgggcctgg cccgcagccg
     1321 cgaccttagc ggcgatgatc ggatcctgcc gccccgctcc gaagaggggg agggtgacgg
     1381 tcacgccggc cgagaccata tcgccgaaca tcggatctcg gcgttgatag gccacctccc
     1441 agctccagtc ggggcgcttg ccggcctggg ccaggcggac gtcggcctgc gcctgcgccg
     1501 ccatcgcggt ctgctcctct aggcgaggat gcccttccag cgccgcgcgc agggcagggc
     1561 cgttcacctc gaaactgggc ggcgatccgg ccgcctcggg gtcagggtcg ccggtccagc
     1621 gggacagctc ggcgcgcgct cgcccgacag tcgcggtgac ttcgctccgc cggtcttcca
     1681 gatccgcccg catctgttcg gcttccaggg actgggccgg gcgggccgcc cccgacgcca
     1741 ccgccgaggg tgcggcgctc cagagcggtt cgaggcgggt caaaacctcg cctacggccg
     1801 cctgccgtcg ttcagcgtag tagaggttga tccaggccag ggccgtggcg gttcggacct
     1861 cgc
//
LOCUS       DAOMEO010000683         1863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6479_length_1863_cov_2.776549, whole genome shotgun sequence.
ACCESSION   DAOMEO010000683 DAOMEO010000000
VERSION     DAOMEO010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_1863_cov_2.776549"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /gene="pdeM"
                     /locus_tag="PLH31_14010"
     CDS             <1..573
                     /gene="pdeM"
                     /locus_tag="PLH31_14010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HQR90345.1"
                     /translation="KGSSYASRFGQMLPPYDTRETLDRLDREIAALSPARLIFLGDSF
                     HDGAGEDRLADDDAERLRGLARGRELIWAVGNHDEDGPRVLPGEVIAEASILGLTLRH
                     EPEAGEQPGEVAGHLHPAARVSSGRGSVRRRCFVTDGSRLILPAFGAYTGGLNVLDRA
                     FSGLFGGPVLAGALGTQKVHAIGPRSLRPD"
     gene            complement(575..>707)
                     /locus_tag="PLH31_14015"
     CDS             complement(575..>707)
                     /locus_tag="PLH31_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HQR90346.1"
                     /translation="FLLQWVWDVTDHDGPAWRPRMRTLLTSGAAVSLAFALEQALHM"
     assembly_gap    708..717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<718..1053)
                     /locus_tag="PLH31_14020"
     CDS             complement(<718..1053)
                     /locus_tag="PLH31_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HQR90347.1"
                     /translation="MSEPVADRVPAPPAVWVFGLATLIPFFVASGLFCYGPPGVRSGA
                     LISLLAYSTAMVSYFGGVRSGLEIENPSPRWAVLGLSLLFPLAGFGLLLGELRFAPAW
                     QLSGFLLVFL"
     gene            complement(1192..>1863)
                     /locus_tag="PLH31_14025"
     CDS             complement(1192..>1863)
                     /locus_tag="PLH31_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HQR90348.1"
                     /translation="KAANLGLPRAQFYLGKMYEMGEGGVKKDLVEARRWTERAATAGD
                     ARAMHNLALFYFKGEGGAKNSTTAANWFRKAADQGLVDSQFNLAQLYEGGWGVSQNPA
                     EAYKWYVIAGRAGDATSKARANALRPQLTAEAQRTADRSALAFRAQTVNASVAANTAA
                     PSNAGLGTAQRVLSQLGYYQGPRDGVASPALRMAIAAYQRDQGLPSSGALDGETLNRL
                     SVYTR"
BASE COUNT          311 a          614 c          658 g          270 t
ORIGIN      
        1 aaaggcagca gctacgcctc gcgttttggc cagatgctgc cgccctatga cacccgcgaa
       61 accctggatc gcctggaccg cgaaatcgcg gcgctgtcgc cggcgcgcct gatctttcta
      121 ggcgacagct tccatgacgg cgcgggcgag gatcggctgg ccgatgacga cgccgaacgc
      181 ctgcgggggc tggcccgagg acgcgagctg atctgggcgg tcggcaacca cgatgaagac
      241 ggcccccgcg tcctgcctgg agaagtgatc gccgaggcca gcatcctggg cctgaccctc
      301 cgccatgagc cggaggcggg cgagcagccc ggcgaggtcg ccggacacct gcacccggcc
      361 gccagggtgt cgagcgggcg cggcagcgtg cggcgacgtt gtttcgtgac cgatggatcg
      421 cggctgatcc tgccggcctt cggcgcctat acgggcggac tgaatgtgct ggatcgggcc
      481 ttttcaggcc tgttcggcgg tcccgtcctg gcgggggccc tgggcacgca gaaggttcac
      541 gcgatcggac cgcgctcact ccgtcccgac tagattacat gtgcagggct tgttcgagcg
      601 cgaaggccag cgagacggcg gcgcccgagg tgagcagggt tcgcatccgg gggcgccagg
      661 ccgggccgtc gtggtcggtg acgtcccaga cccattgcag caggaagnnn nnnnnnncag
      721 gaagaccagc agaaagcccg acaattgcca ggcgggcgcg aaccgcagct cgccgagcag
      781 aaggccaaag ccggccagcg ggaacagcag cgagaggccc agaaccgccc agcgcggact
      841 agggttttcg atctccagcc cgcttcgcac gcccccgaaa taggaaacca tcgcggtcga
      901 ataggcgagc agggagatca acgcgccgct gcgcacgccg ggagggccgt agcagaacag
      961 gcccgaggcc acgaagaacg gtatcagggt cgcgagcccg aacacccaaa ccgccggcgg
     1021 cgccggtacg cgatccgcca ctggctcgct catgcatcaa ctcctccggg aaggccgccc
     1081 ccgcgacctc cctaaccccg gcgtgactat aaccagacat accgcgaggt ccacggacga
     1141 tatttgccga atggattcag cgcccccgca atggggtgag ctcggtgtcg atcagcgcgt
     1201 atagaccgaa agccggttca gggtttcgcc gtccagcgcg cccgacgacg gcaggccttg
     1261 atcgcgctgg taggcggcga tggccatgcg cagggcgggg gaagccacgc catcgcgcgg
     1321 tccttgatag tagcccagtt gcgagagcac gcgctgggcg gtgcccaggc ccgcgttcga
     1381 cggcgcggcg gtgttggccg ccaccgaagc gttcacggtc tgggcgcgga aggccagggc
     1441 cgaacggtcg gcggtgcgct gggcctcggc ggtcagttgc gggcgcagcg cgttggcgcg
     1501 ggccttggag gtcgcgtcac cggcgcgacc ggcgatcacg taccacttgt aggcctcggc
     1561 cgggttctgg ctgacgcccc aaccgccttc ataaagctgg gcaaggttga actggctgtc
     1621 gaccagaccc tgatcggcgg ccttgcggaa ccagttggcg gccgtggtcg agttcttggc
     1681 gccgccttcg cccttgaaat agaacagggc caggttgtgc atggcgcgcg cgtcgccggc
     1741 ggtggcggcg cgttctgtcc agcggcgggc ctcgacgagg tccttcttca cgccgccctc
     1801 gcccatctcg tacatcttgc cgagatagaa ctgggcgcgc ggcaggccca gattggcggc
     1861 ctt
//
LOCUS       DAOMEO010000684         1861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6491_length_1861_cov_2.040975, whole genome shotgun sequence.
ACCESSION   DAOMEO010000684 DAOMEO010000000
VERSION     DAOMEO010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_1861_cov_2.040975"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLH31_14030"
     CDS             complement(<1..459)
                     /locus_tag="PLH31_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQR90349.1"
                     /translation="MTNLPFFSPYRHGFVRVATAVPKVKLVDPAANAASVLDLVRDAD
                     AAGVAVLVFPELGLTGYSIDDLLQQDALLDAVEAAIATLAKASEKLAPLFVVGAPLRV
                     DGRLYNTAVAIQGGKVRGVVPKSFLPNYREFYERRWFTPGAGVVGKTVTLA"
     gene            626..>1861
                     /locus_tag="PLH31_14035"
     CDS             626..>1861
                     /locus_tag="PLH31_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519108.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HQR90350.1"
                     /translation="MPVSPIKMADAIRVLSMDAVHKAKSGHQGMPMGMADVATVLWSK
                     FLKFDASKPDWADRDRFVLSAGHGSMLIYSLLHLTGFKAMTMKEIENFRQWGSLTPGH
                     PEVHHTPGVETTTGPLGQGLATAVGMAMAEAHLASRFGKDIVDHRTWVIAGDGCLMEG
                     VSHEAISIAGRLKLSKLTVLFDDNNTTIDGVATISETGDQVARFKAAGWAVKVVDGHD
                     HGKIAAALRWATKQDRPTMIACKTLISKGAGPKEGDPHSHGYTLFDNEIAASRVAMGW
                     DAAPFTVPDDIAKAWKSVGRRGARVRKAWEAGLKASAHAAEFTRAMSGGLPTGAFEAV
                     DAHIAKMAEAKPANATRVHSGAALDHLIPAIPEMIGGSADLTGSNNTFVKGMTAFDTP
                     DYAGRYVHYGVREFGMAAAM"
BASE COUNT          335 a          653 c          600 g          273 t
ORIGIN      
        1 ggccagggtc acggtcttgc cgaccacgcc agcgcccggc gtgaaccagc ggcgctcgta
       61 gaactcgcga tagttgggca ggaagctctt ggggaccaca ccccgcacct tgcccccctg
      121 gatggccacg gcggtgttgt agagccggcc gtccacgcgc agcggcgcgc cgaccacgaa
      181 cagcggcgcg agtttttcgc tggcctttgc cagggtggcg atcgccgcct caacggcgtc
      241 cagcagggcg tcctgctgca ggaggtcgtc gatcgaatag ccggtcaggc ccagttccgg
      301 aaacaccagc accgccacgc ccgccgcgtc cgcgtcgcgc accagatcca gcacgctggc
      361 ggcgttggcg gccggatcga ccagcttgac cttgggcacc gccgtggcga cccgcacgaa
      421 gccgtggcgg tagggcgaga agaacggcag gttcgtcaag cgatcgacct ttcgaggtta
      481 tgctgaaaat gagcataagt ctgggtccag ctataacgtg tccggacggc cttgcctagc
      541 gccgcgcggc ttgaccgaaa ggggtcatcg tgcgacctcg cgcctcgcgc agccctgcaa
      601 aacataacga acaaagacaa cgcccatgcc cgtttcgccc atcaaaatgg ccgacgcgat
      661 ccgcgtcctt tccatggatg cggtccacaa ggccaagtcc ggacaccagg gcatgccgat
      721 gggcatggcc gatgtggcga ccgtgctgtg gagcaagttc ctcaagttcg acgctagcaa
      781 gcccgactgg gccgaccgcg accgcttcgt gctgtcggcc ggccacggct cgatgctgat
      841 ctattcgctg ctgcatctga ccggcttcaa ggccatgacg atgaaggaga tcgagaattt
      901 ccgtcagtgg ggctcgctga cccccggcca cccggaagtg catcacacgc ccggcgtcga
      961 gaccaccacc ggtccgctgg gccagggcct ggccaccgcc gtcggcatgg ccatggccga
     1021 ggcccatctg gcctcgcgct tcggcaagga catcgtcgat caccgtacct gggtgatcgc
     1081 tggcgacggc tgcctgatgg agggcgtcag ccatgaagcc atcagcatcg ccggccgcct
     1141 gaagctgtcc aagctgacgg tgctgttcga cgacaacaac accaccatcg acggcgtcgc
     1201 caccatctcc gagaccggcg accaggtcgc gcgcttcaag gccgccggct gggcggtcaa
     1261 ggtcgtcgac ggccacgacc atggcaagat cgccgccgcc ctgcgctggg ccaccaagca
     1321 ggaccgtccg acgatgatcg cgtgcaagac gctgatctcc aagggcgcgg gtccgaaaga
     1381 aggcgacccc cacagccacg gctacaccct gttcgacaac gagatcgccg cctcgcgcgt
     1441 cgccatgggc tgggacgccg cgccgttcac cgtgccagac gatatcgcca aggcctggaa
     1501 gagcgtcggt cgtcgcggcg cccgcgtccg caaggcctgg gaagcggggc tgaaggcctc
     1561 agcccacgcc gccgagttca cgcgcgccat gtcgggcggc ctgccgacgg gcgccttcga
     1621 agcggtcgac gcgcacatcg ccaagatggc cgaggccaag ccggccaacg ccactcgcgt
     1681 tcactccggc gcggccctgg accacctgat cccggcgatc ccggaaatga tcggcggctc
     1741 ggccgacctg accggctcca acaacacctt cgtcaagggc atgacggcgt tcgacacgcc
     1801 cgactatgcc ggccgctatg tccactatgg cgtgcgcgag ttcggcatgg cggcggcgat
     1861 g
//
LOCUS       DAOMEO010000685         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6495_length_1860_cov_1.797784, whole genome shotgun sequence.
ACCESSION   DAOMEO010000685 DAOMEO010000000
VERSION     DAOMEO010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6495_length_1860_cov_1.797784"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..480
                     /locus_tag="PLH31_14040"
     CDS             121..480
                     /locus_tag="PLH31_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HQR90351.1"
                     /translation="MNETLRTNFSNRLQTAADAKKALLEKFKPKPAVVDPNFESRDAV
                     KAAELQQVRADRAEERAAARQVAADKVAAAEQAIADSAAAVLSLKRGATKERKALSAA
                     EAKAKRDSKYAARKARN"
     gene            647..>1161
                     /locus_tag="PLH31_14045"
     CDS             647..>1161
                     /locus_tag="PLH31_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR90352.1"
                     /translation="MSASATGPHRRALLTGVTALAFAGAARASVATPSRHSVLVKTLI
                     GQMTLDEKIGQLNQAPGGRQRALNSRIDAAQLDLVRKGGIGSYLHVAGADFLRELQRV
                     AVQESRLKIPLLFGIDVIHGFRTIFPVPIGMAATFDPEIVKACSRVAAIETSVSGLHW
                     TFTPMVDIARDP"
     assembly_gap    1162..1171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1172..>1505
                     /locus_tag="PLH31_14050"
     CDS             <1172..>1505
                     /locus_tag="PLH31_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR90353.1"
                     /translation="GEDPYLGSRIAEAQITGLQGDSLAGKDVILACAKHFGAYGAANG
                     GRDYDSADVSERTLQEVYLPPFHASVRAGAGSFMTAFNDIAGVPTTANQALVRGQLRD
                     QWGYQGLVV"
     assembly_gap    1506..1515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1516..>1860
                     /locus_tag="PLH31_14055"
     CDS             <1516..>1860
                     /locus_tag="PLH31_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HQR90354.1"
                     /translation="AQAAALALKAGVDMDMAGGVYAGHLKIAVAADPSLMPYLDQAVG
                     RVLVTKARLGLFDDPFRFGDAAREKTELNSPAHRAIAGEAARKAIVLLKNEGGLLPLS
                     PAGKRIAVIGALA"
BASE COUNT          325 a          644 c          576 g          295 t
ORIGIN      
        1 agcttcaagc tgcctaagcc acttcggagc gcgggccagt cccgcgctcc agcccggctt
       61 ttctaccgag cgacctcgaa ctgacacgcg gggttatccc ccttcgtaat ccgaggccct
      121 atgaacgaaa ctctacgcac caacttctcg aaccgactcc agaccgccgc cgacgccaag
      181 aaggcgctac tggaaaagtt caagcccaag cccgccgtcg ttgatcccaa cttcgagagc
      241 cgcgacgccg tcaaggccgc cgagcttcag caagttcgtg ctgatcgcgc cgaagaacgc
      301 gccgccgccc gccaggtcgc agccgacaag gttgccgccg ccgaacaagc catcgccgac
      361 agcgccgccg ccgtgctcag cctgaagcgt ggcgcgacca aggaacgcaa ggccctgtcc
      421 gccgccgaag ccaaggccaa gcgcgacagc aagtatgccg cccgcaaggc gcgcaactag
      481 cgttcagctc ctaagagcac gatgaaggcc gggtcacacc ggccttttta cgatcactgt
      541 ttgacagcgt tgtcagaaca ttgaacttct gtagtgtgcc gccacggcct tggccgaatg
      601 gacgcaaaga gctccgcttc acaggagccg ctacgggaga ccagggatgt cggccagtgc
      661 tacaggaccc catcgccgcg ccttgctgac gggcgtcacc gccctggcct tcgccggcgc
      721 cgcccgcgcg tcggtcgcga cgccatcgcg gcactcggtg ctggtcaaga cgctgatcgg
      781 ccagatgacg ctggacgaaa agatcggcca gctgaatcag gctcccggcg gccgtcaacg
      841 cgccctcaac tcgcggatcg acgcagccca gctggatctg gtgcgcaagg gcggcatcgg
      901 ctcgtacctg cacgtagccg gggcggactt tctgcgcgaa ctgcagcgcg tggcggttca
      961 ggagagccgg ctgaagatcc cgctgctgtt cggcatcgac gtgatccacg gcttccgaac
     1021 catcttcccc gtgccgatcg gcatggcggc gacctttgat ccggagatcg tcaaggcctg
     1081 ctcacgtgtc gcggcgatcg agacctcggt cagcggcctg cactggacct ttaccccgat
     1141 ggtcgacatc gcccgcgacc cnnnnnnnnn ncggcgaaga tccctatctg ggctcacgaa
     1201 tcgccgaagc ccagatcacc ggcctgcaag gcgacagcct ggccggcaag gatgtgatcc
     1261 tggcctgcgc caaacacttc ggagcctatg gcgcggccaa tggcggccgc gactatgaca
     1321 gcgccgacgt ctccgagcgc acgctgcagg aggtctatct gccgcctttt catgcctcgg
     1381 tgcgggcggg ggcaggcagc ttcatgaccg ctttcaacga catcgccggc gtgccgacca
     1441 ccgccaatca ggccctggtg cgcggccagc tgcgcgacca gtggggctat cagggcctgg
     1501 tggtcnnnnn nnnnncgccc aggctgccgc gctggcgttg aaggccggtg tcgacatgga
     1561 catggctggc ggcgtctatg ccggccacct gaagattgcc gtcgccgccg atccgtcgct
     1621 gatgccctat ctcgatcagg ccgtcggcag ggtgctggtg accaaggcgc ggctgggcct
     1681 gtttgacgac cccttccgct ttggcgacgc ggctcgcgag aagaccgagc tcaattcccc
     1741 cgcccatcgc gccatcgccg gcgaggcggc gcgcaaggcc atcgtgctgc tcaagaacga
     1801 gggcggcctg ctgcctttgt cgccggcagg caagcgtatc gctgtgattg gcgccctggc
//
LOCUS       DAOMEO010000686         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6497_length_1859_cov_2.334812, whole genome shotgun sequence.
ACCESSION   DAOMEO010000686 DAOMEO010000000
VERSION     DAOMEO010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_1859_cov_2.334812"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLH31_14060"
     CDS             complement(<1..436)
                     /locus_tag="PLH31_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene hydroxylase"
                     /protein_id="HQR90355.1"
                     /translation="MATLALNLALFLAAFVFMEGFAWVTHRYVMHGFMWVWHKSHHEP
                     RHGMFELNDLFAVVFAAPAIVAIYFGVHGTPWLLPVGLGITAYGFMYFVFHDGMVHRR
                     FKTPFGKSAFWKRAIQAHRIHHAVHTKEGAVSFGFLLARPVRE"
     gene            complement(445..1653)
                     /locus_tag="PLH31_14065"
     CDS             complement(445..1653)
                     /locus_tag="PLH31_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR90356.1"
                     /translation="MIRLDMTLAMVRVMALELWRDRAALVMTFVLPPLVFLIFSSVFA
                     GATGEDIKPKLALVDLARTETSGRVAKALLASSEIRAEATTPATVEAVRALVKSGQAD
                     VGLVIRADPAAPGRPFLIISDPARAVAAPLTQARVQLAMARAAPDAALRRSVDQLTPA
                     LGDLTPAQTARLDAAADALKAKPPAVTGGGLFDREDLLGAKKGGATIAYYAGAVMILF
                     ALFSAMQGSLGLMDERRTGVADRLLAGVAGMGPVVTGKFVFLVAQGVIQAVAIFATAQ
                     IIYGVAVVEHFALWLPTTLATAVCAAGLALGLVSVCRTREQAQMLSTFVILVLAAIGG
                     SMVPRFLMPVWLQTVGWFTPHAWVIDAYQGVLWRDAPLSQVYKAWGVLTAAGLAGLAL
                     AQTLARLSRR"
     gene            complement(1650..>1859)
                     /locus_tag="PLH31_14070"
     CDS             complement(1650..>1859)
                     /locus_tag="PLH31_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR90357.1"
                     /translation="GETLLAAEGLARTAHRAKLWTRLDVDGYAAAGRLDKRLRKAGLI
                     PREIRVREPSLQNLFSLVAERKLAA"
BASE COUNT          354 a          647 c          624 g          234 t
ORIGIN      
        1 gctcgcgcac cggcctggcc agcaggaagc caaacgacac ggccccctcc ttggtgtgaa
       61 cggcgtgatg gatccggtgc gcctggatag cgcgcttcca gaaggctgac ttgccgaagg
      121 gcgtcttgaa gcgtcggtgc accatgccgt cgtggaagac gaagtacatg aagccatagg
      181 cggtgatgcc caggcccacc ggcaacagcc acggtgtgcc gtgaacgcca aaatagatcg
      241 ccacgatggc cggggccgca aagaccacgg cgaacagatc gttcagctcg aacatgccat
      301 gtcgcggctc atgatgcgac ttgtgccaga cccacatgaa gccgtgcatc acatagcgat
      361 gcgtgaccca ggcaaagccc tccatgaaga cgaaggcggc gaggaaaagc gcgagattga
      421 gagccagggt cgccatcaac aatcctatcg acgcgacagt cgcgccaggg tctgggccag
      481 cgctaggccg gccaggccgg cggcggtcag cacgccccaa gccttataca cctgcgacag
      541 tggcgcgtcg cgccagagca cgccttgata ggcgtcaatc acccaggcgt gaggcgtgaa
      601 ccagcccacc gtctgcagcc agaccggcat caggaagcgc ggaaccatgc tgccgccgat
      661 cgcggccaga accaggatca cgaaggtcga cagcatctgg gcctgttccc gcgtgcggca
      721 caccgagacc aggccgaggg ccaggcccgc cgcgcagacc gccgtcgcca gggtggtggg
      781 cagccacagg gcgaaatgct cgaccaccgc cacgccatag atgatctggg cggtggcgaa
      841 gatggctacg gcctggatga cgccctgtgc gaccaggaag acgaacttcc cggtcaccac
      901 cggccccatg ccggccaccc cggccagcag acggtcggca acgccggtgc gacgctcgtc
      961 catcaggccc agcgaaccct gcatggccga gaacagggca aacaggatca tcaccgcgcc
     1021 ggcgtaatag gcgatggtcg ccccgccctt cttggcgccc agcaggtcct cgcggtcgaa
     1081 aagaccaccg ccggtcacgg ccggaggctt cgccttcagc gcatccgcgg cggcgtcaag
     1141 gcgagccgtc tgggccggcg tcaggtcgcc caacgcgggg gtcagctgat cgacgctgcg
     1201 gcgcagggcg gcgtcgggcg cagcgcgcgc catggccagt tggacgcgag cctgggtcag
     1261 gggcgcggcc acggcccggg cggggtcgga tatgatcagg aacggacggc ccggcgcggc
     1321 gggatcggcg cggatcacca gacccacatc ggcctggcca gacttcacca gcgcccgcac
     1381 cgcctcgacc gtggccggag tcgtggcctc ggcgcggatc tcggaactgg ccagcagggc
     1441 cttggccaca cgacccgagg tttccgtgcg cgccagatcg accagagcca gcttgggctt
     1501 gatgtcctca cccgtcgcgc cagcaaacac cgacgagaag atcaggaaca cgagcggcgg
     1561 cagcacgaag gtcatcacca gcgcggcgcg gtcgcgccac agctccagcg ccatgacccg
     1621 caccatggcc agcgtcatgt ccagccggat catgcggcca gcttccgctc ggcgacgagg
     1681 ctgaacaggt tctgcagcga cggctcccgc acccggatct cgcgcgggat tagccccgcc
     1741 ttgcgcagcc gcttgtccag tcgcccggca gcggcgtagc cgtcgacatc gaggcgggtc
     1801 cagagcttgg cgcggtgggc ggtccgcgcc agaccttcgg ccgccagcag cgtctctcc
//
LOCUS       DAOMEO010000687         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6498_length_1859_cov_2.273836, whole genome shotgun sequence.
ACCESSION   DAOMEO010000687 DAOMEO010000000
VERSION     DAOMEO010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6498_length_1859_cov_2.273836"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLH31_14075"
     CDS             complement(<1..372)
                     /locus_tag="PLH31_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR90358.1"
                     /translation="MPSKRISFVLAAVACSALTACGTSMPKVSTEGNYATPIGKAPVT
                     ANPTDYSAALVCLNQYAKQNRIGAPRVAIGRIADYTGKEESDGSGRKVTQGASLLAMS
                     AFAKAGMPMVERFDTSVSEFEL"
     assembly_gap    375..384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<385..644)
                     /gene="hfaA"
                     /locus_tag="PLH31_14080"
     CDS             complement(<385..644)
                     /gene="hfaA"
                     /locus_tag="PLH31_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holdfast anchoring protein HfaA"
                     /protein_id="HQR90359.1"
                     /translation="MAWQIKNTIGFGAGVTAAVVGLGALGAAGAQSMSSNSAAYNAGY
                     GRLAGQESRMVEYSTRDANGNRVVVDGVMLTGADQSVYSSSNS"
     assembly_gap    963..972
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <973..1444
                     /locus_tag="PLH31_14085"
     CDS             <973..1444
                     /locus_tag="PLH31_14085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQR90360.1"
                     /translation="LVTSLFVHGCWIHALMNAAFALAFGAPVARLLGLGVRGGGLFGL
                     LYIACGVLAGLGYAAIHPQSTSLVVGASGAVAGLMGGAARLLDRPGQLSSLFAPRALS
                     LALGWLVINLVMAAFGGFPGLTGAVAWEAHLFGFAAGALLISPAAWLAPRSETH"
     gene            1503..>1859
                     /locus_tag="PLH31_14090"
     CDS             1503..>1859
                     /locus_tag="PLH31_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR90361.1"
                     /translation="MLVSQILKDKGDLVFTASPQETVGAAAALLHTRRVGAMVVLDGD
                     ETIVGILSERDIVRVIAKEGAGALNKPITACMSADVIFAHPEESIDILLERMTDRRIR
                     HLPVVRDGRLAGIISIG"
BASE COUNT          274 a          585 c          623 g          357 t
ORIGIN      
        1 cagttcgaac tccgacaccg aggtgtcgaa gcgctcgacc atcggcatgc cagccttggc
       61 gaaggcgctc atggccagca gcgaggcgcc ttgcgtgacc ttgcggcccg agccgtcgga
      121 ctcttccttg ccggtatagt cggcgatgcg gccgatcgcg acgcgcggcg cgccgatgcg
      181 gttctgcttg gcgtattggt tcaggcacac cagggcggcc gaatagtcgg tcggattggc
      241 ggtcaccggg gccttgccga tcggcgtggc gtagtttccc tcggtcgaca ccttgggcat
      301 gctggttccg caggccgtca gggcgctgca ggccacggcc gccaggacaa aagagatacg
      361 cttacttggc atcgnnnnnn nnnngaattg ctcgacgaat agacgctctg gtcggcgccg
      421 gtcagcatca cgccgtcgac cacaacgcgg ttgccgttgg cgtcgcgggt cgaatattcg
      481 accatgcggc tttcctgacc ggccaggcgg ccatagccgg cgttgtaggc ggccgagttg
      541 ctggacatcg actgtgcgcc ggccgcgcca agcgcgccga ggcccacaac ggcggcggtg
      601 acgcccgcgc cgaacccgat ggtgttctta atttgccagg ccatgagagc ctccgcgact
      661 gccgacgcga aggtgcaagc aaccctcgtg ccaaattcgt acagtctctg gtctagcgcc
      721 aaggtctcgc aaaatggtta agcgggagag cgtgcgactc tgaatcgcgc gacgacgtct
      781 tatgtaagag cgtgatgacc ccagacccct ccttcgacct tggtcaagac cgccctgaac
      841 cgatgttcaa cgcgccgtgg cccgccgtgc tggcggccgc cagtattgtc atctcgaatt
      901 tcgtcctggg ctgggtcagt gacgagacga tctaccgtct cgccatggag cctgtgcggg
      961 tgnnnnnnnn nnactggtga cctcgctctt tgtccatggc tgctggatcc atgctctgat
     1021 gaacgccgcc ttcgccctgg ccttcggcgc gcccgtcgcg cggctgctgg ggctgggagt
     1081 caggggaggg ggcctgtttg ggcttctcta tatagcgtgc ggcgtgctgg cggggctggg
     1141 ctacgccgcc atccatccgc aatcgaccag cctggtggtc ggcgcctctg gcgcggtggc
     1201 cggcctgatg ggtggcgcgg cgcgtctgct ggatcggccc ggacagctga gcagcctctt
     1261 tgcgccgcgc gccctgtcgc tggcgttggg ctggctggtc atcaatctgg tcatggcggc
     1321 gtttggcggt tttcccggcc tgaccggcgc ggtggcctgg gaggcgcatc tgttcggctt
     1381 cgccgccggg gcgctgctga tttctcccgc cgcctggctg gccccgcgtt ccgagaccca
     1441 ttgatccccg ggccgctttg cgagaccctc gttattataa gaacgaacaa gggagacgcg
     1501 cagtgctggt ttcacagatc ctgaaggata agggcgatct ggtgttcacg gcctcgcccc
     1561 aggagacggt gggcgccgca gcggccctgc ttcatacccg ccgggtgggg gccatggttg
     1621 tgctggacgg tgatgagacc atcgtcggca tcctttccga gcgcgacatc gtgcgggtga
     1681 tcgccaagga aggcgcaggc gccctgaaca agccgatcac cgcctgtatg agcgccgatg
     1741 tgatcttcgc tcaccccgag gaatccatcg acatcctgtt ggagcgcatg accgaccgtc
     1801 gcatccgcca cctgccggtg gtgcgcgacg gacggttggc cgggatcatc tcgatcggc
//
LOCUS       DAOMEO010000688         1859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6499_length_1859_cov_2.196231, whole genome shotgun sequence.
ACCESSION   DAOMEO010000688 DAOMEO010000000
VERSION     DAOMEO010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_1859_cov_2.196231"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1383
                     /gene="dnaJ"
                     /locus_tag="PLH31_14095"
     CDS             238..1383
                     /gene="dnaJ"
                     /locus_tag="PLH31_14095"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQR90362.1"
                     /translation="MRDYYEILGVTRTADDAGLKAAFRKLAMEHHPDRNGGCEDASGR
                     FKEINEAYSVLSDPQKRAAYDRFGHAGVNGGQGGGGFSGDPSDIFSDVFGDVFGEMFG
                     GGRRPSNGPQRGQDLRYDLEISLEQAYAGAEVEIKVPAAITCEVCDGSGAKVGTSPTV
                     CGTCGGHGRVRATQGFFAVERGCPRCGGSGRLVLDPCQSCNGHGQVRRERVLSVRIPA
                     GVDDGARIRLSGEGDAGARSGPRGDLYIFLSVIPHELFERDGLDLLCTVPVPMTVAAL
                     GGEIDAPCLMGGEECDGTCKIPVKVPEGAQTGKTVRLKGKGMPSLRSRQRGDLVVELF
                     VETPTHLSARQKELLKELAGLCGENQNPTSANFVGKAKRFWDEVTGS"
     gene            complement(1390..>1859)
                     /locus_tag="PLH31_14100"
     CDS             complement(1390..>1859)
                     /locus_tag="PLH31_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR90363.1"
                     /translation="WAYPAHAQRKEIDGKAVINCKVGYDGLLRDCRIVSEAPAGEDFG
                     VAALALSQKFRMIPPEAGADSLPDVTIPVVFQVPRSEPFQPKTAGEASPVFTLLQLFR
                     VGLVKEEQRGPAKPPQPLPAHDQKVVAGIAAVAFVLLVAMVLGLGHRRKPRRP"
BASE COUNT          295 a          630 c          630 g          304 t
ORIGIN      
        1 cggtgacgcc gctgcggacg atggcgtggt cgacgccgag ttcgaggaag tcgacgaaaa
       61 caagccggcg aactagttcg ccatgcgcgc gccgcggaac gtttctgaca tgatcgttcc
      121 gtggccgcgt ttgcttgatc cgcgcgcttg tgacgccttg cgtcgctcgc cggaaaccac
      181 cacctctttt cccatcaccg cctctcccgt gggcctcttg ggcgccagac gctgacgatg
      241 cgcgactatt acgaaatcct gggcgtgacc cggaccgccg acgatgcggg cctgaaggcc
      301 gcgttccgca agctggcgat ggagcaccat cccgaccgca atggcggttg cgaggatgcg
      361 tccggccgct tcaaggaaat caacgaggcc tattcggtcc tgtccgatcc ccagaagcgc
      421 gcagcctatg accggttcgg ccatgccggc gtcaacggcg gccagggcgg cggcgggttc
      481 agcggcgatc ccagcgacat cttcagcgat gtctttggcg atgtgttcgg cgaaatgttc
      541 ggcggcggcc gtcgcccgtc caacggcccc cagcgcggcc aggacctgcg ctacgatctg
      601 gagatctcgc tggagcaggc ctatgccggc gccgaggtcg agatcaaggt gcccgccgcc
      661 atcacttgcg aggtctgcga cggctcgggc gccaaggtcg gcaccagccc gacggtctgc
      721 ggcacctgcg gcggccacgg ccgcgtgcgg gccacgcaag gcttcttcgc ggtcgagcgc
      781 ggctgccccc gctgcggcgg ttcgggccgg ctggtgttgg acccctgcca gagctgcaat
      841 ggccacggcc aggtgcgccg cgagcgcgtc ctgtcggtgc gcatccccgc cggcgtcgat
      901 gacggcgccc gcatccgcct gtcgggcgaa ggcgacgccg gcgcccgcag cggcccgcgc
      961 ggcgacctct acatcttcct gtcggtgatc ccgcacgagc tgttcgagcg cgatggcctg
     1021 gatctgctgt gcaccgtgcc cgtgccgatg accgtggccg ccctcggcgg cgagatcgac
     1081 gccccctgcc tgatgggcgg cgaggaatgc gacggcacgt gcaagatccc cgtcaaggtt
     1141 cccgaaggcg cccagaccgg caagaccgtc cgcctgaagg gcaagggcat gccgtccctg
     1201 cgcagccgcc agcgcggcga cctggtggtg gaactgtttg tcgaaacccc gacccacctg
     1261 tcggcccggc aaaaggagct cctcaaggag ctggctggcc tgtgcggcga gaaccagaac
     1321 cccacgtcgg ccaacttcgt cggcaaggcc aagcggttct gggacgaggt tacggggagc
     1381 tagccggctc tagggcctgc gcggtttgcg gcggtgtccc aggccaagga ccatggctac
     1441 aagcaggacg aaagccacgg cggctatccc ggccacgacc ttttggtcgt gagcgggtaa
     1501 aggttgcggt ggtttagcgg gcccgcgctg ctcctctttg accagcccga cacgaaacaa
     1561 ctgcaacagc gtgaagactg gcgacgcctc gcccgctgtt ttgggctgga acggctcgct
     1621 ccgagggacc tggaacacga ccggtatggt gacatccggg aggctgtcag ctccggcttc
     1681 gggcgggatc atccggaact tctgcgacag tgccagcgcc gcgacgccga aatcctcgcc
     1741 ggccggcgct tctgatacta ttcgacaatc gcgcagcagg ccgtcatagc cgaccttgca
     1801 gttgatcacg gccttgccgt cgatctcctt gcgttgggca tgggcgggat aggcccact
//
LOCUS       DAOMEO010000689         1857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6506_length_1857_cov_2.237514, whole genome shotgun sequence.
ACCESSION   DAOMEO010000689 DAOMEO010000000
VERSION     DAOMEO010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_1857_cov_2.237514"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..426)
                     /locus_tag="PLH31_14105"
     CDS             complement(175..426)
                     /locus_tag="PLH31_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90364.1"
                     /translation="MSTTLVTQLEAALCDIAGVESRPSSLTVDYGPDGPEGERADDLA
                     VRAWVERSTRSLVFAQGEARRRNGSLAATASAVFRRVGA"
     gene            complement(589..927)
                     /locus_tag="PLH31_14110"
     CDS             complement(589..927)
                     /locus_tag="PLH31_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90365.1"
                     /translation="MKLRTLAAVAALSVSMFAGAALADGRVAAALEAPVAAKTKIVAG
                     GAVFVCDGGECVAASAPSRAITAASCKALAKEVGRVAAFGGETKSLDAEDLTRCNVSA
                     KAGVSQTAAK"
     gene            complement(1167..1583)
                     /locus_tag="PLH31_14115"
     CDS             complement(1167..1583)
                     /locus_tag="PLH31_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90366.1"
                     /translation="MATAAIIGAALGLFVRPRLLGVLVAVLLVGAVEGGVLMLIGMLE
                     GQPNRELLIARLEAVFGEGLLGAAGPIAATFIGAVLSALMGSFSDPSDSPGLLTADQL
                     RRRVGKNGRYARIEGMVEERQIHAKAESRIDSILGL"
BASE COUNT          401 a          596 c          584 g          276 t
ORIGIN      
        1 atccgcccgg tgcgttgcaa tggccgcgga caagaagatt aaccctcgtc ccggcttaaa
       61 ggcttgagcg cgcttgaaaa aagcacatgg agggccagcg ctgatgcgcc accccgtccg
      121 aagccgcgcg cctgataaca cgggcttttc gcagtcgcaa ccaaaggggc gcgatcaggc
      181 cccgacgcgg cgaaacaccg ccgaggcggt ggccgcgagg cttccgttac ggcgccgggc
      241 ctcgccctgc gcgaagacca ggctgcgcgt tgaccgctcc acccaggcgc gcaccgccaa
      301 atcgtcggcg cgctcgccct ccgggccgtc tggaccatag tcgacggtca gggacgacgg
      361 gcggctctca acgcctgcga tatcgcacaa cgcggcttcc aactgggtaa cgagggtggt
      421 gctcatgctt tttcactacg caggatcgag aaaaagaaat tcactacgca ggatcgagaa
      481 aaagaaaaag ggcggcgaga ctggctcgcc gccctgtcag gcgaatgctc gaggaaacag
      541 ccagacgggg cgcaggtcgc cccaacacgt ccgggtacgg gtaaagcctt acttcgcagc
      601 ggtctggctg acgccggcct tggccgagac gttgcagcgg gtcagatcct cagcgtccag
      661 agacttggtc tcaccgccga aagcggcgac ccggccgact tccttggcca gggccttgca
      721 cgacgccgcc gtgatggcgc gcgacggagc ggaagcggcg acgcattcac cgccgtcgca
      781 gacgaagacg gcgccgccag cgacgatctt ggtcttggcg gcgacgggcg cttccagggc
      841 ggcagcgacg cggccgtcag cgagggcggc gccagcaaac atggagacgg acagggcggc
      901 gacagccgca agggtacgga gcttcataag agccctccaa cgttcgggga gttgtgaaaa
      961 gatcgatgat cggcgaaaag cgtactggtc gttcaggctt cgaggatgca agcgtcggta
     1021 tccccaccga cgcgacgaaa ctgaactaag cagtgcttag atatcagtga tcaacgattt
     1081 cgcaagcgga atttatttcg cgtgagcaat ttgtaatcaa acccagtcga tttcggaaat
     1141 ggccagccat caactctggc tgggcgctag aggcccagga tcgagtcgat ccggctctcg
     1201 gccttggcgt ggatctgccg ctcttcaacc atgccctcga tccgcgcgta gcgaccgttc
     1261 ttgcccaccc ggcggcgaag ctggtcagca gtcagcaggc ccggcgagtc actgggatcg
     1321 gaaaagctgc ccatcagcgc cgagagcacc gcgccaatga aggtcgcagc tatgggcccc
     1381 gctgcgccga gcaagccctc gccgaacaca gcctcgaggc gggcgatcag cagttcgcga
     1441 ttgggctggc cttccagcat gccgatcagc atcagaacgc caccctcgac ggcgccaacc
     1501 agcagcaccg ccaccagcac gcccagcagt cggggccgga cgaacagtcc gagcgccgcg
     1561 ccgatgatcg ctgctgtggc catggtgaac atgaaccaag actagcaggg cttggtcaaa
     1621 agcccgttaa gaccaaaggt attcccaagc ggcagggttg gcggcgatcc gacgccgcgc
     1681 atactcgccc ccaagacaaa agaaaaatat cacagggagg ctaggccatg gacttcaacc
     1741 cgccgccgga actgaccgcc tatctggccg tcctcgacag attcatcgag gacgagatcg
     1801 cgcccttgca ggcccgcgat gacaatgagc gcttcttcga ccaccgccgc gaatggg
//
LOCUS       DAOMEO010000690         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6511_length_1855_cov_2.297222, whole genome shotgun sequence.
ACCESSION   DAOMEO010000690 DAOMEO010000000
VERSION     DAOMEO010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_1855_cov_2.297222"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLH31_14120"
     CDS             <1..387
                     /locus_tag="PLH31_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90367.1"
                     /translation="EAMTDATVRMVRRSALSQLAAQRPEVARALFRLTVEGLQRSQDH
                     VLMLGRKSARERVVSLLIDLANRTGADGELDVPMSRQDMADYLGVTIETVSRTLTQLQ
                     VDGVIAIPTTRHIVLRDSAALRRFAA"
     gene            complement(429..>1429)
                     /locus_tag="PLH31_14125"
     CDS             complement(429..>1429)
                     /locus_tag="PLH31_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90368.1"
                     /translation="HFAAPMVIFVLFLMARALFGLFGSASNPATQAYMAERTSREERT
                     QSMATLAGAFGLGTVVGPLLAPLFVLPIVGLAGPMFAFAILAGVMVIIVQRGLPETFK
                     PSRGETPPRRGFGFPFKGEGAGLWKDPRLKPFLIYGFLVASCQTAQSQTLGFLIIDKL
                     QLPPMQAQGYITLAMAAGAVAGLLAQWGLIRMFAMRPRDLMRWGAGAAVVGNVIVAFA
                     PDYAAVAIGFAIANLGYGFARPGFTAGASLSVGAKDQAGAAGAIAAINGLNVVFGPLF
                     VMLYRWIGWGPFVLNAVILAGLLVYTFRQAALRKVSEADPQEEATLASLERSDEGG"
     assembly_gap    1430..1439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1440..1736)
                     /locus_tag="PLH31_14130"
     CDS             complement(<1440..1736)
                     /locus_tag="PLH31_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90369.1"
                     /translation="MSTAAPDARSNRGAFAILFGVSVVSAMGNSGMWSVLPAIGREIG
                     IPDVLVAAIFSLSAVLWAISSPFWARQSDIHGRKPLILLGLVGFCVSMILCAIVV"
BASE COUNT          344 a          631 c          623 g          247 t
ORIGIN      
        1 gaagcgatga cagacgccac ggtgcgcatg gtgcgtcgct ccgcgctcag ccagctggcc
       61 gcccagcggc ccgaggtggc ccgggcgctg tttcgcctga cggtcgaagg cctgcagcgc
      121 agccaggacc atgtgctgat gctgggccgc aagtcggcgc gcgaacgcgt ggtcagcctg
      181 ctgatcgatc tggccaaccg caccggcgcc gacggggaac tggatgtgcc gatgtcgcgc
      241 caggacatgg ccgactatct gggcgtgacc atcgagacgg tgtcgcgcac gctcacccag
      301 ctgcaggtcg acggcgtgat cgccattccc accacgcgcc acatcgtcct gcgcgatagc
      361 gcggccttgc gccggttcgc ggcctaggct ttccagccag gcggaacgta gcacaggggg
      421 acaaaggcct agccgccctc gtcgctgcgc tcgaggctgg ccagggtcgc ctcctcctgg
      481 ggatcggctt cgctgacctt tcgcagagcc gcctgacgga atgtgtagac cagcaggcct
      541 gccaggatga cagcgttgag cacgaacgga ccccagccga tccagcgata gagcatcaca
      601 aacagcgggc cgaacaccac attgaggccg ttgatcgccg cgatcgcccc ggcggcgccg
      661 gcctgatcct tggcccccac cgacagcgag gccccggcgg tgaagcccgg ccgcgcgaag
      721 ccgtagccca gattggcgat ggcgaagccg atggcgacgg cggcgtagtc cggcgcgaag
      781 gcgacgatca cattgcctac aaccgccgcc ccggcgcccc agcgcatcag gtcgcgcggt
      841 cgcatggcga acatgcggat caggccccac tgggccagca ggccggccac cgcgcccgcc
      901 gccatcgcca gggtgatgta gccctgggcc tgcatgggcg gcagctgcag cttgtcgatg
      961 atcagaaagc ccagggtctg ggactgggcc gtctggcatg aggcgaccag gaagccgtag
     1021 atcaggaagg gcttgagccg tggatccttc caaaggccgg ccccctcgcc cttgaacgga
     1081 aagccaaagc cgcgccgggg cggcgtttcg ccgcggctgg gcttgaaggt ctcgggcagg
     1141 ccccgttgaa cgatgatcac catcacgccg gccaggatgg cgaaggcgaa catcgggccg
     1201 gccaagccca cgatgggaag cacaaacagc ggcgccagga gcggtcccac caccgtgccc
     1261 aggccaaacg ccccggccag ggtggccatg ctctgggtgc gctcctcgcg gctggtgcgc
     1321 tcggccatat aggcttgggt ggcggggttg gacgccgagc cgaacaggcc aaacagcgct
     1381 cgcgccatca ggaacagcac gaagatcacc atcggcgcgg cgaagtggtn nnnnnnnnng
     1441 acgacgatcg cgcacaggat catcgacacg cagaaaccga ccagacccag caggatcaag
     1501 ggcttgcggc cgtggatgtc ggactgccgg gcccagaacg gcgagctgat cgcccacagc
     1561 acggccgaca gcgaaaagat cgccgccacc aggacgtccg gaatgccgat ttcccgcccg
     1621 atggccggca gcaccgacca catgccgcta ttgcccatgg ccgagaccac cgacactcca
     1681 aagaggatcg cgaacgcgcc ccggttgctc cgggcgtctg gtgcggcggt ggacatcaaa
     1741 caactgttag gccgctcgcc gccgccgcgc aactcggcga gccgcgtccg ggaaccttgg
     1801 attaatcatt aaccgggatg atgcgggatc acttctcggg gtcggcctta cgtca
//
LOCUS       DAOMEO010000691         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6520_length_1852_cov_2.406233, whole genome shotgun sequence.
ACCESSION   DAOMEO010000691 DAOMEO010000000
VERSION     DAOMEO010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6520_length_1852_cov_2.406233"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PLH31_14135"
     CDS             complement(<1..153)
                     /locus_tag="PLH31_14135"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQR90370.1"
                     /translation="MAWLRDIDHWFATRVLPFAGNYRAYARKLIGDEAEAEDLVQEAY
                     AKVLGVP"
     gene            complement(244..1092)
                     /locus_tag="PLH31_14140"
     CDS             complement(244..1092)
                     /locus_tag="PLH31_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90371.1"
                     /translation="MSDNSPRSADRKFALIAPVVVGLAAVVVLVLLLTRQPPPPPASP
                     PVVAEAPPIVAAPPALPAVATRAELIAAAARASDAYAAGTTEGGGRDLVGRRFRVRLT
                     FGCGGPAADPGSAQAYYQYDPAGSALRLVARPAVWTDLPLVRAAPTPSGTESVEGFWL
                     PRPWLTSEGCPSRRTVAPPATPTSVEAPTLGLAMLHAAEASRVERRGKRPYEHVVKLA
                     PENLDQAQTFGLVLEGKVSGFADGAAIHCWSESAEHRPVCLYAVDIDRVAFEDAKAEI
                     LADWPR"
     gene            1186..>1852
                     /locus_tag="PLH31_14145"
     CDS             1186..>1852
                     /locus_tag="PLH31_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQR90372.1"
                     /translation="MTPAQIIALGVLAGVVVALIHGKLRSDVVALAGAAVLLGTRTVR
                     PVEVQAAFASPAILALASLFVIAYAMELSGLLGVLIRGATRLCARLGAVGLWLVIAFC
                     GLASAFLNNTPIVVLGAPVVRDVARSMDLSPKRFLIPLSYATILGGACTLIGTSTNLL
                     VNDMARNAGQPAFSLFEITPVALVIAAVGGLYLVLVGPRLLGAEPAASPASEPMARPP
                     NSPP"
BASE COUNT          282 a          653 c          627 g          290 t
ORIGIN      
        1 cggcacgccc agcaccttgg cataggcttc ctggacaagg tcttccgcct cggcctcgtc
       61 gccgatcaat ttgcgggcat aggcgcgata gttgccggca aagggcaaca cgcgggtcgc
      121 aaaccaatga tctatgtcac gcagccaggc catatcgcgt cctgtcgcct gagacagggt
      181 caggtcgact aggcgctgca ctagcgcgat cacgtgtcag tttgctgacg ggccagaccc
      241 agcctatcgc ggccagtcgg caaggatctc agccttggcg tcctcgaagg cgacgcggtc
      301 aatgtcgacc gcatacaggc agaccggtcg atgctcggct gattcactcc agcagtggat
      361 cgccgcccca tcggcgaaac ccgaaacctt gccctccaga accagaccga acgtctgcgc
      421 ctgatcaagg ttctcagggg cgagcttgac aacatgttcg taggggcgct tgccgcgtcg
      481 ctcaacgcgc gaggcttcgg cggcgtgcaa catggccagg cccagcgtgg gcgcttcgac
      541 cgacgtgggg gtggcgggcg gcgccacggt gcgacgcgaa gggcagccct ccgaggtcag
      601 ccaggggcgc ggcagccaga acccttcaac cgattccgtc cccgagggcg tcggcgcggc
      661 gcgcaccagc ggcaggtccg tccacaccgc ggggcgcgcg accagccgca gcgccgaacc
      721 ggccgggtca tactgataat aggcctgggc cgaaccggga tcagcggccg gtccgccgca
      781 tccaaatgtc agacgcaccc gaaaccggcg gcccaccaga tctcgtccgc cgccttctgt
      841 ggtcccggcg gcataggcgt cagacgcccg cgcagccgca gcgatgagct cggcccgcgt
      901 ggcgaccgca ggaagcgcgg gaggcgctgc gacgatcggc ggggcctcag cgaccacagg
      961 cggcgaagca ggcggcggcg ggggctggcg ggtcagcagc agcacaagca ccacgacggc
     1021 cgcgagcccg acgacgacgg gcgcgataag ggcgaacttg cggtctgccg acctggggct
     1081 attgtcgctc atggctacag cctagcagaa cacagggtcc cctttgcaat cgccttcgtc
     1141 ccgatccgcg cgggtcgcgt gcctccgcgt cggcgcgcga cgttcatgac cccagcccag
     1201 atcatcgccc tgggagtgct ggcgggagtc gtcgtcgccc tgatccacgg caagctgcgc
     1261 tccgatgtgg tggccctggc tggcgccgcg gtgctgctgg gcacgcgcac cgttcgtccg
     1321 gtcgaggttc aggccgcctt cgccagtccg gcgatcctgg ccctggcctc gctgttcgtc
     1381 atcgcctacg ccatggagct gtcgggcctg ctgggcgtgc tgatccgggg cgcgacacgg
     1441 ctctgcgccc gcctgggcgc ggtgggcctg tggctggtca tcgccttctg cggactggct
     1501 tcggcgtttc tcaacaacac gccgatcgtc gtgctgggcg cgcccgtggt gcgcgacgta
     1561 gcccgctcga tggacctgtc gcccaagcgc tttctgatcc ccctgtcgta cgcgacgatc
     1621 ctgggcggcg cctgcaccct gatcggcacc tcgaccaatc ttctggtcaa cgacatggcc
     1681 cgcaatgccg gccagccggc gttcagcctg ttcgagatca cgccggtggc cctggtcatc
     1741 gccgcggtcg gcggcctcta tcttgtgctg gtgggaccgc gactgctggg cgccgagccg
     1801 gcggcgtcac ccgcatccga gccgatggcg cgacctccga actcgccgcc cg
//
LOCUS       DAOMEO010000692         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6525_length_1851_cov_2.089644, whole genome shotgun sequence.
ACCESSION   DAOMEO010000692 DAOMEO010000000
VERSION     DAOMEO010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6525_length_1851_cov_2.089644"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLH31_14150"
     CDS             complement(<1..1134)
                     /locus_tag="PLH31_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR90373.1"
                     /translation="MGHAHHQHRPGCTHGPATADSAKTIDPVCGMTVNPETTANKASH
                     EGQPFFFCSAGCRAKFVADPQKYLADKPTAAESAPAWTIYTCPMHPEIRQVGPGSCPI
                     CGMALEPDLITADAGPNPELADMTRRFWIGLVLTVPVFALEMGGHLTGLMMRLSPATS
                     GWIQLVLATPVVLWAGWPFFERGARSVVTRNLNMFTLIAMGIGVAWIFSVVAVLAPDV
                     FPTAFRSADGSAPLYFEAAAVITVLVLLGQLLELRAREQTSGAIRALMDLAPKTARRV
                     RSDGTDEEVTLDLITVGDSLRVRPAEKVPVDGEVLEGRVSIDESMVTGESMPVTRGPG
                     DKVVGGAINKTGSFVMRAEKVGADTLLSQIVQMVAQAQRSRAPI"
     gene            1315..>1851
                     /locus_tag="PLH31_14155"
     CDS             1315..>1851
                     /locus_tag="PLH31_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90374.1"
                     /translation="MPPIDVQRRRDQVLNRECFCITLDSQGLQAAIRAQLPAETGSES
                     LVTNHPHLFAQAPVFLAREDLEAMLDVVGAIEAAAATSAYQDAVLTWAPAIAREDFGP
                     RGAFMGYDFHLTDDGPKLIEVNTNAGGAFLNALLARAQTACCEEARVAARSAQSEEFE
                     PAVWRMFMSEWESQRGPLP"
BASE COUNT          334 a          590 c          602 g          325 t
ORIGIN      
        1 aataggcgcg cggctacgct gcgcttgggc caccatctga acgatctggg acagcagagt
       61 gtcagcgccg accttttcag cgcgcatgac aaacgagccg gtcttgttga tcgcgccgcc
      121 gaccaccttg tcacctggcc cgcgggtgac gggcatcgac tcgcccgtca ccatcgattc
      181 gtcgatcgac acgcgacctt cgagcacctc accgtcgact ggaacctttt cagcaggccg
      241 aacacgcagg ctgtcgccaa cggtgatgag atctagggtg acctcttcat cggttccgtc
      301 gctgcgcacc cgacgggcgg tcttgggcgc taggtccatc agcgcgcgga tcgcccccga
      361 tgtctgctcg cgggctctca gctcgagcaa ttggcccagc aggacgagga ccgtgatgac
      421 ggcggcggct tcgaaataaa gcggcgcgct gccatcggcg gatcggaagg cggtcggaaa
      481 gacgtccggc gccaagacgg ccacgacact gaatatccag gcgacgccga tgcccatggc
      541 gatgagcgtg aacatattga gattgcgggt aacgacagag cgcgcgccgc gctcgaagaa
      601 gggccagcca gcccagagca cgaccggggt cgccagcacc agttggatcc agcccgaggt
      661 ggcggggcta agacgcatca tcaggccggt gaggtgcccg cccatctcca gggcgaagac
      721 cggaacagtc agcacaaggc cgatccagaa ccggcgcgtc atgtcggcca gctcaggatt
      781 ggggcccgcg tcagccgtga tcaagtccgg ctccagcgcc atgccgcaaa tggggcagga
      841 cccgggccct acctggcgga tctccgggtg cataggacag gtgtagatgg tccaggcggg
      901 ggcgctttct gcggcggtcg gcttatcggc caggtatttc tgcgggtctg ccacgaactt
      961 cgcgcggcat cccgccgagc agaagaagaa gggctgccct tcgtgcgacg ccttgttggc
     1021 cgtcgtctcg gggttcaccg tcatgccgca gacaggatcg atcgtcttgg cgctgtcggc
     1081 ggtcgccggt ccgtgcgtgc agcctggtcg gtgctgatgg tgggcatgtc ccattgggtc
     1141 ggccatatca gactccttga ggccgccccg gacggccgcc aaatcaaccc tcaggcctcc
     1201 catcatggga aggtcaaggg ttcttttcct ttaggcacaa cgcacgggat ggcttgaagc
     1261 agcggatccc ccttagccca cgcgtccctg gtccgggcgt gctagaattc agtgatgcca
     1321 ccgattgacg ttcaaagacg gcgcgaccaa gtgctgaaca gggaatgctt ctgcatcact
     1381 ctggacagcc agggcttaca ggccgcgatc cgcgcccaat tgcccgccga aaccggctcc
     1441 gaatccctcg tcactaatca cccgcatctc ttcgcgcagg cgccggtgtt tttggcccgc
     1501 gaggacctgg aagccatgct cgatgtggtt ggcgccattg aggccgccgc agcgacgtca
     1561 gcttatcaag atgcggttct cacctgggcg ccggcgatcg cccgcgagga ctttggaccg
     1621 cgcggcgcct tcatgggcta tgactttcac ctaaccgatg atggtcccaa gcttatcgag
     1681 gtcaatacca acgctggtgg cgccttcctg aacgccttac tggcccgcgc gcagacggcc
     1741 tgctgcgagg aagctcgggt ggcggcgcga tcggcgcagt cggaggagtt tgagccggcc
     1801 gtctggcgca tgttcatgag cgaatgggag agccagcgtg ggccactgcc g
//
LOCUS       DAOMEO010000693         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6531_length_1850_cov_2.384958, whole genome shotgun sequence.
ACCESSION   DAOMEO010000693 DAOMEO010000000
VERSION     DAOMEO010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6531_length_1850_cov_2.384958"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLH31_14160"
     CDS             complement(<1..1007)
                     /locus_tag="PLH31_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQR90375.1"
                     /translation="MKLLRRLAWTLGIVALTVAAFTIWVIWPGKAPVPPPLVTDPPPP
                     WEQQGATQYIPSVDPPAAAPFTARNEACDTGAAPPGAAAVNAASLTTMVWSPFGRPEV
                     GWEIYAPRIAAEIDTACAPDTRRFAALLAKWQQSRGLKPTGVMDLSAFQPMLTGWHLQ
                     RPFVRVNNTGICPGAPLLSGLNTAKPNESYGGKTIQLRPGALEAYRRMVAEARAAGVI
                     QRPETLTIFSGFREPAADAARCERDNNCQGLVRTICSAHRTGLALDIYIATAPGFGPD
                     SSADDNRRAMARSAVFRWMVVNAGRFGFVNYAYEPWHWEWTGEPLLPGVPIKSLSQAG
                     SA"
     gene            1088..>1445
                     /locus_tag="PLH31_14165"
     CDS             1088..>1445
                     /locus_tag="PLH31_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90376.1"
                     /translation="MPLDPRAVALILGCALATAACSDPAPEPPAAASPVAPPAPTPAQ
                     TDMPVVDAPPAVVAASASPQAQAIDAAIFDPAVTDPEAIKRAMTRAQILLDRAHMSPG
                     VIDGRPGTNVTLALEAF"
     assembly_gap    1446..1455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1456..>1850
                     /locus_tag="PLH31_14170"
     CDS             <1456..>1850
                     /locus_tag="PLH31_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90377.1"
                     /translation="LPVNGKLDKVSWDALVAGDAGPVMTDYLITPEDVAGPFQPNIPK
                     DDYEAMAKLPSLGYGTPLEALAERFHMDEALLQALNPGVDFTAAGTAIVVAAAGPDRL
                     AGKVARIEIDKARGQVRVFDDKAVQLAVY"
BASE COUNT          282 a          660 c          613 g          285 t
ORIGIN      
        1 gccgaccctg cctgggacag gctcttgatc ggtacgcccg gcagcagcgg ctcgccggtc
       61 cactcccagt gccagggctc gtaggcgtag ttgacgaagc caaaccggcc cgcgttgacg
      121 accatccacc ggaagaccgc cgagcgggcc atggcgcggc gattatcgtc ggccgagctg
      181 tcggggccaa agcccggggc ggtggcgata tagatgtcca gggccagccc ggtgcggtgg
      241 gccgagcaga tggtccgcac caggccctgg cagttgttgt cacgctcgca gcgcgcggcg
      301 tcggcggccg gctcgcggaa gccggagaag atggtcagcg tctcgggccg ctggatcacc
      361 ccggcggccc tggcctcggc caccatccgg cgataggcct ccagcgcgcc gggccgcagc
      421 tggatcgtct tgccgccata gctttcgttc ggcttggcgg tgttcagtcc cgacaacagc
      481 ggcgcgccgg ggcagatccc ggtgttgttg acccgcacga acggccgctg caggtgccag
      541 cccgtcagca tgggctggaa agcgctcagg tccatcacgc cggtcggctt cagaccacgc
      601 gactgctgcc atttggccag caaggccgcg aaccggcgag tatccggggc gcaggcagtg
      661 tcgatctcgg cggcgatgcg cggcgcgtag atttcccagc cgacctcggg acgcccgaac
      721 ggcgaccaga ccatcgtggt gagcgacgcg gcgtttacgg ccgcagcgcc tggcggggcg
      781 gcgcctgtgt cacaggcttc gttgcgcgcc gtgaagggcg cagccgccgg aggatccacc
      841 gaagggatat attgtgtcgc accctgctgc tcccacgggg gcggcgggtc ggtgaccagc
      901 ggtggcggaa ccggagcctt gcccggccag atcacccaga tcgtgaaggc ggccaccgtc
      961 agggcgacta tgcccaatgt ccaggccagg cggcgcagca gcttcaaccg tttctcccca
     1021 ggatcattgg aacttagcca tgactgaagg cttcagatcg gcagtcgttc cgtctggagt
     1081 ttccaccatg cccctcgatc cgcgcgccgt cgccctgata ctcggttgcg ccctcgccac
     1141 ggcggcctgt tcggatccgg ctccggaacc gcctgccgcc gcatcgcctg tcgccccgcc
     1201 cgcgccgacc ccagcccaga ccgatatgcc cgtggtcgac gccccgcccg ccgtcgtcgc
     1261 ggcctcggct tcgccccagg ctcaggccat cgacgcggcg atctttgatc cggccgtcac
     1321 cgatcccgag gcgatcaagc gggcgatgac ccgcgcccag atcctgctcg accgcgccca
     1381 catgtcgccg ggcgtcatcg atgggcgacc cggaaccaat gtcactctgg cgcttgaagc
     1441 cttccnnnnn nnnnncctgc cggtcaatgg caagctggac aaggtttcgt gggacgccct
     1501 ggtcgctggc gacgccgggc cggtgatgac cgactatctc atcacccctg aagatgtcgc
     1561 cggtcccttc cagcccaaca tccccaagga cgactatgaa gccatggcca agctgcccag
     1621 ccttggctac ggcacgccgc tggaggccct ggccgagcgc ttccacatgg acgaggcgct
     1681 gctgcaggcc ctcaatcccg gcgtcgattt cacggccgcc gggaccgcca tcgtcgtcgc
     1741 cgccgctgga cccgacaggc tggccggcaa ggtggcgcgc atcgagatcg acaaggcgcg
     1801 cggccaggtc cgcgtcttcg acgacaaggc ggttcagctg gccgtctatc
//
LOCUS       DAOMEO010000694         1843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6556_length_1843_cov_1.779642, whole genome shotgun sequence.
ACCESSION   DAOMEO010000694 DAOMEO010000000
VERSION     DAOMEO010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6556_length_1843_cov_1.779642"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>426
                     /locus_tag="PLH31_14175"
     CDS             <1..>426
                     /locus_tag="PLH31_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR90378.1"
                     /translation="EAFIDALLAGPKAEFKEWEHVPYFDGCLPIEVMAERGRETLRHG
                     PMKPVGLTNPRDPLVKSYAIVQLRQDNALGTLWNMVGFQTKLKHGAQAEVFRMIPGLK
                     DAQFARLGGLHRNTFINSPRLLDRSLRMKVEPRLRFAGQM"
     assembly_gap    427..436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <437..756
                     /locus_tag="PLH31_14180"
     CDS             <437..756
                     /locus_tag="PLH31_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HQR90379.1"
                     /translation="SAATGLLAGRFAAAERLGRTLDAPPPTTALGALVDHVTGGHIEG
                     EVEPGKTSFQPMNINYGLLPPMEAPKTGEDGVKIPLEERGRAKKRLMSLRALSDLEAW
                     LDA"
     gene            980..>1843
                     /locus_tag="PLH31_14185"
     CDS             980..>1843
                     /locus_tag="PLH31_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR90380.1"
                     /translation="MKDRSAHPRVRALFGQASIGALVVASALSLGSTAFAQDAAATAA
                     EPEAVEEIVVTGYRAALQSALNLKRNSNVMVDAINAEDIADFPDANLAESLQRLPGVS
                     VDRENGEGRTITVRGLGSDFTRVRLNGLEALSTAGPSIAGDAANRSRGFDFNTFASEL
                     FNSLKVQKTASAETDEGSLGATVDLETGRPLSFKGRRLALSLQNAYYENGGTNSPRFA
                     GLVSDRWETNWGDFGALFSVAYNKRNQIIDSYQRQAGQSEYTYRGSTFATTPNPTSGT
                     YRLTRQGFATPI"
BASE COUNT          328 a          649 c          559 g          297 t
ORIGIN      
        1 gaggccttta tcgacgccct gctggccggc cccaaggccg agttcaagga atgggagcac
       61 gtcccctatt tcgacggctg cctgcccatc gaggtgatgg ccgagcgcgg tcgcgagacc
      121 ctgcgccatg gcccgatgaa gccggtcggc ctgaccaatc cgcgcgaccc gctggtgaag
      181 tcctacgcca tcgtccagct gcgccaggac aacgccctgg gcacgctgtg gaacatggtc
      241 ggcttccaga ccaagctgaa gcacggcgct caggccgagg tgttccggat gatcccgggc
      301 ctgaaggacg cccagttcgc ccggctgggc ggtctgcacc gcaacacctt catcaacagc
      361 ccgcgcctgc tggaccgcag cctgcggatg aaggtcgagc cgcgcctgcg cttcgccggc
      421 cagatgnnnn nnnnnnaaag cgccgccacg ggcctgctgg ccggccgctt cgccgccgcc
      481 gagcgcctgg gtcggacgct ggacgccccg cctccgacga cagccctggg cgctttggtc
      541 gaccacgtca ccggcggcca cattgaaggc gaggtcgagc ccggcaagac cagcttccag
      601 ccgatgaaca tcaactatgg cctgctgccg ccgatggaag cgcccaagac cggcgaggac
      661 ggcgtcaaga tccccctcga ggagcgcggg cgggcaaaga agcggctgat gagcctgcgc
      721 gccctgtcgg atcttgaggc ctggctggac gcctgaaccc agccttccgg ccttgtctga
      781 tcggcaggcc ccgctgtttg gcgcagagcc acgcgtgtgt cctgaaggcg tctgacctcg
      841 ctcaaatatc aatgttcgcc agagatgaca ccggtttctc ttgcaaatga caccggtggc
      901 ctttagcgct atttcgactg actgaggcgt gaccgaccca aaaagagtcg cgccttttgt
      961 gaactttcgg gagggaacta tgaaggatcg atccgcacat ccgcgcgtgc gcgcgctttt
     1021 cggccaggcg tccatcggcg ccctggttgt cgcctcggcc ctgtctctgg gctcgaccgc
     1081 cttcgcacag gacgccgccg cgacggccgc cgaacctgaa gccgtcgaag aaatcgtcgt
     1141 caccggctat cgggcggctc tccagagcgc attgaacctc aagcgcaatt cgaacgtgat
     1201 ggtcgacgcc atcaacgctg aagacatcgc cgactttccc gacgccaacc tcgccgagtc
     1261 cctgcagcgc ctgccaggcg tttcggtcga ccgcgaaaac ggcgaaggcc gcaccatcac
     1321 cgtccgcggc ctgggcagcg acttcacccg ggtgcgcctg aacggtcttg aagccctgtc
     1381 caccgccggt ccgtcgatcg cgggcgacgc ggccaaccgc tcgcgcggct ttgacttcaa
     1441 caccttcgcc tcggaactgt tcaactcgct gaaggtccag aagaccgcct cggccgagac
     1501 cgatgaaggc tcgctaggcg cgaccgtcga tctggaaacc ggccgcccgc tgagcttcaa
     1561 gggccgtcgc ctggccctgt ccctgcagaa cgcctactac gaaaacggcg gcaccaactc
     1621 cccgcgtttc gccggcctgg tctcggaccg ctgggaaacc aactggggtg atttcggagc
     1681 cctgttctcg gtcgcctaca acaagcgcaa ccagatcatc gacagctatc agcgtcaggc
     1741 gggccagtcg gaatacacct atcgcggttc gaccttcgcc acgacgccca acccgacctc
     1801 gggcacatac aggctgaccc gccagggctt cgcgacgccg atc
//
LOCUS       DAOMEO010000695         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6558_length_1842_cov_2.367096, whole genome shotgun sequence.
ACCESSION   DAOMEO010000695 DAOMEO010000000
VERSION     DAOMEO010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_1842_cov_2.367096"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLH31_14190"
     CDS             <1..522
                     /locus_tag="PLH31_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR90381.1"
                     /translation="SLAAEVAYKLDSSHTEAMFIIDRFGFNSVIGIFGESEGTIWLDE
                     AAPEKSRVEATIQVGSLLSGHPTRDEHLKAERWLNVAKSPTMSFKSTKVEKTGPDTAK
                     VTGDMTILGVTLPTTLDVKLNKIGKAPNNQKQQAGFTITGAISRKAFGSVTGAGMIGD
                     SVSIRIEALAIAQ"
     gene            complement(525..1295)
                     /locus_tag="PLH31_14195"
     CDS             complement(525..1295)
                     /locus_tag="PLH31_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQR90382.1"
                     /translation="MSAPLAFAAGMASAAPVSRWQAYERRLSRGLSDAAGGDFDDGFE
                     DTLLDLNNIIRERQDLPALRPDHGLTLAARAHAADMAVSGVFDHVTSDGFSAAGRVGL
                     LARDLVGAPGENIAMRRNAAGAVQPDQIMNQWRTSPGHRANLVARGFTHVGYGVLRQG
                     PRVIAVGAYAEVSARLARPAPLRVRSADEIAAALSRATPAIGQFSVSEPGGDVLTTTY
                     AQGRLQPVLRPGAWQLRPHLLSGERRYQVAWGPVFFLD"
     gene            1377..>1842
                     /locus_tag="PLH31_14200"
     CDS             1377..>1842
                     /locus_tag="PLH31_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HQR90383.1"
                     /translation="MRPVRSIWLAGPDVWLPDSQAQAARQRAYCIDAGFTGLTPDPVP
                     PGSEQSSELEARELYAARMARLRQADAGVVNLTPWRGPSADPGAAFEIGVLAGLGRPI
                     FAYLNVPDESQAEYVDRVESFVGAQRDEAGIWRDGDGCVVEDYGLPETVMLWA"
BASE COUNT          315 a          607 c          643 g          277 t
ORIGIN      
        1 agcctggccg ccgaagtggc ctacaagctg gattcttccc acaccgaggc gatgttcatc
       61 atcgaccgct ttggcttcaa ctcggtgatc ggaatctttg gcgagtcgga gggcacgatc
      121 tggctggacg aggccgcgcc ggaaaagtcg cgggtcgagg ccaccattca ggtgggcagc
      181 ctgctgtcgg gccaccccac ccgtgacgag cacctgaagg ccgagcgctg gctgaacgtc
      241 gccaagagcc cgaccatgag cttcaagtcg accaaggtgg aaaagaccgg cccggacacc
      301 gccaaggtga ccggcgacat gaccattctg ggcgtgaccc tgcccacgac cctggacgtc
      361 aagctcaaca agatcggcaa ggcgcccaac aatcaaaagc agcaggccgg cttcaccatc
      421 accggcgcga tcagccgcaa ggccttcgga agcgttacgg gcgcgggcat gatcggcgac
      481 agcgtctcga tccgcatcga ggccctggcg atcgcgcagt agccctagtc cagaaagaac
      541 acgggccccc aggctacctg gtagcgacgc tcgcctgaca agaggtgcgg gcgcaactgc
      601 caggcgccgg gtcgcagaac cggctgcagg cgcccttggg cataggtggt ggtcagcacg
      661 tcgccgcccg gttcggagac cgagaactgg ccgatggccg gcgtcgcccg ggacagggcg
      721 gcggcgatct cgtcggctga tcgcacccgc agcggcgccg ggcgcgccag ccgggcgctg
      781 acctcggcat aggcgcccac cgcaatgacg cgcggaccct ggcgcagaac gccatagccc
      841 acatgggtga agccgcgcgc caccagattg gcgcggtggc cggggctggt gcgccactgg
      901 ttcatgatct ggtcgggctg aacggcgccg gcggcgttgc ggcgcatggc gatgttctcg
      961 cccggcgcgc cgaccagatc gcgggccagc aggccgacgc ggccggcggc gctgaagccg
     1021 tccgaggtga catggtcgaa aacgccgctg accgccatgt cggcggcatg ggcgcgggcg
     1081 gccagagtga ggccgtgatc tggccgcagc gcgggcaggt cctgacgctc gcgaatgata
     1141 ttgttgagat ccagcagggt gtcttcgaac ccgtcgtcga aatcgccgcc ggctgcgtcg
     1201 gagagccccc ggcttaaccg gcgctcatag gcctgccagc gcgaaacggg cgcggcgctg
     1261 gccatgccgg cggcgaaggc cagcggcgcg ctcaacaggg ttcttcgaag gatttgcggc
     1321 atcgcggtcc ttaacagtga ttgacagacg cccctgcgac caagctctaa gacccgatgc
     1381 gtcccgtgcg ttccatatgg ctggccggcc ccgatgtctg gctccccgat tcccaggccc
     1441 aggccgcgcg gcagcgggcc tattgcatcg acgcagggtt taccggcctg actccggacc
     1501 ccgtccctcc gggttcggaa cagagttccg agctcgaagc ccgcgaactc tacgcggccc
     1561 gcatggcgcg attacgtcag gctgacgccg gggtggtcaa tctgaccccg tggcgcggac
     1621 cgtcagctga tcccggcgcg gccttcgaga tcggcgtgct ggccggtctg gggcggccga
     1681 tcttcgccta tctgaacgtg ccggacgaat cccaggctga atatgtggat cgcgtcgaat
     1741 ccttcgtcgg cgcccagcgt gatgaggcgg gtatctggcg ggacggcgat ggctgcgtgg
     1801 tcgaggacta tggcctgccg gagaccgtga tgctgtgggc tg
//
LOCUS       DAOMEO010000696         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6560_length_1842_cov_1.917739, whole genome shotgun sequence.
ACCESSION   DAOMEO010000696 DAOMEO010000000
VERSION     DAOMEO010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_1842_cov_1.917739"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLH31_14205"
     CDS             complement(<1..387)
                     /locus_tag="PLH31_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HQR90384.1"
                     /translation="MAVAPNTGLSAKQRSEIAAGLNKVLADSYALYLKTHGYHWNVRG
                     PNFQSLHVLLEGQYTEQWAALDTIAERIRALGELAPQGYAAFGNLSSIKDGDPEQDWE
                     AMLAELKKDNETVIATLRAAFPTADDA"
     gene            467..1807
                     /locus_tag="PLH31_14210"
     CDS             467..1807
                     /locus_tag="PLH31_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR90385.1"
                     /translation="MSHDAPSSISKERERLHGLISAWVIPTVPPPAWRCAAAAVIAVA
                     AAILMRFALLGLSGGVGATQPFFPAMVLVTLYAGWRWGLVPIACGAAFGWWLWGGRYG
                     EAMSEDEIATMVIFLTSAAVTTVVAEGLRGAVQGLARARLEQRDAEVRLEVVQSAAGV
                     GPWDWDIVTDTLHLSPSARRNLGFSPEEPVDWPRMNAAIHPDDRQMVTDNIRTSVRQG
                     EACEVEFRLADLSQGERWIHARGQVLQNEAGRAIRMVGLNFDVTKRRRDDERLRESEA
                     RFRSLADSAPALMWVSRVDGQREFVNRAYADFAGVDYDAALSLDWRTRLDPEDLPGIL
                     KGQIAGEGSRKPFSLEGRYRRADGQMRWLKSFSQPRLDPGGAFAGFIGIAFDITDAKQ
                     AEADDGDLAEPGVAHFADSHIWRMAAAISSICAAVPMVMRNACGRPWPGSQRVM"
BASE COUNT          265 a          600 c          647 g          330 t
ORIGIN      
        1 cgcgtcgtca gcggttggga aggcggcgcg cagggtggcg atcaccgtct cattgtcctt
       61 tttcagctcg gcgagcatcg cttcccagtc ctgttcgggg tcgccgtcct tgatgctgga
      121 aagatttcca aaggcggcat agccctgcgg ggccagctcg cccagagcgc gaatgcgttc
      181 ggcgatggtg tccagggccg cccactgttc ggtgtactga ccttcgagca ggacgtgcag
      241 ggactggaag ttggggcccc ggacgttcca gtgatagcca tgcgtcttga gatagagcgc
      301 atagctgtca gctagcacct tgttcagtcc ggcggcgatc tcggaccgct gtttagccga
      361 tagtccggtg ttgggcgcga cagccatgat aatctcccgt gttgcgtgtt ctaggtaagc
      421 tcgtccatgc tcttggcaag ccttgtttgg gcggagagcg acaaaggtga gtcacgacgc
      481 cccatcctcg atctccaaag agcgtgaacg cctccacggg ctaataagcg cctgggtgat
      541 cccgaccgtt ccaccgccgg cgtggcgctg cgccgctgcg gccgtcatcg cggtggcggc
      601 ggcgattctg atgcgcttcg ccctgctggg cctgagcggc ggagtcgggg ccacccagcc
      661 cttcttcccg gccatggtgc tggtcaccct gtatgccggc tggaggtggg ggctggttcc
      721 gattgcttgc ggcgcggcct ttggctggtg gctgtggggc ggtcgctacg gcgaggccat
      781 gtccgaggac gagatcgcca ccatggtgat cttcctgacc tcggctgcgg tcaccacagt
      841 ggtcgccgaa ggtctgcgcg gcgccgtgca gggcctggcg cgggcgcggc tggagcagcg
      901 cgacgccgag gttcggctgg aggtggtgca gtccgccgcc ggggtcggcc cctgggactg
      961 ggacatcgtg accgacaccc tgcacctgtc gcccagcgcc cggcgcaacc tgggcttttc
     1021 gccggaagag ccggtcgact ggccccgcat gaacgcggcg atccatcccg acgaccgcca
     1081 gatggtcacc gacaacatcc gcacctccgt gcgccagggc gaggcctgcg aggtcgagtt
     1141 ccgcctggcc gatctgagcc agggcgagcg ctggattcac gcccggggtc aggtgctgca
     1201 gaacgaggcc ggccgcgcca tccggatggt gggcctgaac ttcgacgtca ccaagcgccg
     1261 ccgcgacgac gagcgcctgc gcgagagcga ggcgcggttc cgcagcctgg ccgacagcgc
     1321 cccggccctg atgtgggtgt cgcgggtcga cggccagcgc gaattcgtca accgggccta
     1381 tgccgacttc gcgggcgtcg actatgacgc cgccctgagc ctggactggc gcacgcggct
     1441 ggatcccgag gacctgcccg gcatcctcaa gggccagatc gccggagagg gctcgcgcaa
     1501 gccgttctcg ctggagggac gctatcgccg ggccgatggc cagatgcgct ggctgaaatc
     1561 cttctcccag ccgcgcctgg atccgggcgg cgcctttgcc ggcttcatcg gcatcgcctt
     1621 cgacatcacc gacgccaagc aggccgaggc cgacgatggt gacttggctg aacccggcgt
     1681 cgctcacttt gcggactcgc acatctggcg gatggctgcg gcgatctcgt ccatctgcgc
     1741 tgccgtgccg atggtgatgc gcaacgcttg tggcaggccc tggccgggca gccagcgggt
     1801 gatgtaaccg tggtccatca ggccggtgta gacctgttcg gc
//
LOCUS       DAOMEO010000697         1839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6572_length_1839_cov_2.182735, whole genome shotgun sequence.
ACCESSION   DAOMEO010000697 DAOMEO010000000
VERSION     DAOMEO010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_1839_cov_2.182735"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1067)
                     /locus_tag="PLH31_14215"
     CDS             complement(177..1067)
                     /locus_tag="PLH31_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90386.1"
                     /translation="MRLASLAAAALVLIAAGHAAAAPKTFRQLLDMPRAAPTAHIPYG
                     AEPEQFGDLWLPEGPGKHPVVVLIHGGCWQAKYPSVELMAYMARDLQGRGYAVWNLAY
                     RRLGEPGGGYPGTFVDVANGLDKLRELAPEHRLDLKRVVVAGHSAGGHLTTWAAGRPR
                     LPKASPLRVGKPLKIRGAVSLAGINDLAAYRATGPDACGGPDTIDQLTAGAALPDRYA
                     DTSPPAFLPIKTPLAVISGALDPIVPPVFGVEFAAKAKGAGDRVEEITIADAGHFELI
                     DPTSAAWTRIVGVIDAMARK"
     gene            complement(1129..1839)
                     /locus_tag="PLH31_14220"
     CDS             complement(1129..1839)
                     /locus_tag="PLH31_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQR90387.1"
                     /translation="MLALSARGATVLIGDPGRAAMLQALLDGRAQPASALAWAAGLTA
                     QAASNHLTKLVEGGLLKVERQGRHRYYRLHNAEVAHALEALALLAAPIRALEQPRSPL
                     ARALRGGRCCYGHLAGRLGVGLTRALEDHGHILAADDKLYAISPAGTAWFAELGVDVA
                     TLRSKRGVARQCLDWTERQHHLAGPLGVALCDRLEALGWIVRDRASRAAELTPAGRDA
                     LREHLNLDLDAEAVARAA"
BASE COUNT          261 a          624 c          707 g          247 t
ORIGIN      
        1 gcccggtccc gaacacggcc tgcggagcct ccttgagggc gggaacccct gtttcagctt
       61 gcacgtgaag cgccagcgaa gtgcgcgcgc acttcgcccc tcccgcactt gcggcggtac
      121 gaggggttcc cgccacaagg gcgggaatga cgggaaaaaa ggggaagcgt gcgaccctat
      181 ttgcgagcca tcgcgtcaat cacccccacg atccgcgtcc atgccgccga ggtgggatcg
      241 atcagctcga agtgcccggc gtcggcaatg gtgatctcct cgaccctgtc gcccgcccct
      301 ttggccttgg ccgcgaactc cacgccaaat accggcggca cgatcggatc cagcgcgccg
      361 gagatcaccg ccagcggcgt cttgatcggc aggaaggccg gcggcgaggt gtcggcatag
      421 cgatcaggca gggcggcgcc ggcggtcagc tggtcgatgg tgtcgggtcc gccacaggcg
      481 tcggggccgg tggcgcgata ggcggccagg tcgttgatgc cggccagact gaccgcgccc
      541 cggatcttca gcggcttgcc gacgcgcagg gggctggcct tgggcaggcg cgggcggccc
      601 gccgcccagg tcgtcaggtg cccgcccgcc gaatgaccgg ccaccaccac gcgcttgagg
      661 tccagtcgat gctcgggcgc cagctcacgc agcttgtcca ggccgttggc cacgtcgacg
      721 aaggtgccgg gatagccgcc gcccggttcg cccagccggc gataggccag gttccacacc
      781 gcatagccgc gtccctgcag gtccctggcc atgtaggcca tcagctcgac gctgggatat
      841 ttggcctgcc agcagccgcc atggatcagc accacgaccg gatgcttgcc gggtccctcg
      901 ggcagccaaa gatcgccgaa ctgctcgggc tcggcgccat aggggatgtg cgcggtgggc
      961 gcggcgcgcg gcatgtccag cagttggcgg aaggtcttgg gcgcggcggc ggcgtgacca
     1021 gccgcgatca ggacgagggc ggcggcggca aggctggcga ggcgcatgga gggtctttcg
     1081 tcgaacggag cggcagttgg ccccgcgcga cgggcgcgcg tcaacggttc aagcggcgcg
     1141 cgcgacggcc tcggcgtcga ggtcgaggtt caggtgttcc cgcagcgcgt cgcgccccgc
     1201 cggggtcagt tcggcggcgc ggctggcgcg gtcgcggacg atccagccca gggcctccag
     1261 gcggtcgcac agggccacgc ccagcgggcc ggccaggtga tgctggcgct cggtccagtc
     1321 caggcactgc cgggcgacgc cgcgtttgga gcgcagggtg gccacgtcga cgccgagctc
     1381 ggcaaaccag gcggttccgg cggggctgat ggcatagagc ttatcgtcgg ccgcaaggat
     1441 gtggccgtgg tcctccagcg cgcgggtcag gcccacgccc agccgtccag ccagatggcc
     1501 atagcagcag cggccgccgc gcagggcgcg ggccaggggc gagcggggct gttccagggc
     1561 gcggatcggg gcggccagca gggccagggc ctccaaggcg tgggcgacct cggcgttgtg
     1621 caggcggtaa taacggtggc ggccctggcg ctcgaccttc agcagtccgc cctcgaccag
     1681 tttggtcagg tggttgctgg ccgcctgggc ggtcaggccg gcggcccagg ccagggcgct
     1741 cgcgggctgg gcgcggccgt ccagcagggc ctgcagcatg gcggcgcggc cggggtcgcc
     1801 gatcagcacg gtggcgccac gggcggagag cgccagcaa
//
LOCUS       DAOMEO010000698         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6577_length_1838_cov_2.164330, whole genome shotgun sequence.
ACCESSION   DAOMEO010000698 DAOMEO010000000
VERSION     DAOMEO010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6577_length_1838_cov_2.164330"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLH31_14225"
     CDS             complement(<1..294)
                     /locus_tag="PLH31_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR90388.1"
                     /translation="MMQSHAWGAKVVDASQVFKPVGPGPFPVALILHGCGGKTPFLEA
                     YAQTAVDAGYAAIVVDSLKPRGMSTLDAKLLVCTGVRLHGLKRAADLFAVLAWL"
     gene            complement(302..1837)
                     /gene="purH"
                     /locus_tag="PLH31_14230"
     CDS             complement(302..1837)
                     /gene="purH"
                     /locus_tag="PLH31_14230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQR90389.1"
                     /translation="MLSVSDKTGLVEAARVLHEAGVELVSTGGTKAAIAAAGLPVKDV
                     SDLTGFPEMMDGRVKTLHPLVHGGLLGVRDAPEHVAAMVEHGVEAIDILYVNLYPFEA
                     TVAKGGSYAECVENIDIGGPAMIRSAAKNHGYVAVCTDPADVAEVLEALKAGGATSFG
                     LRQQLAARAYARTAAYDAAISAWFAAALGQDFPARKAIAGELRQVMRYGENPHQKAAF
                     YAFPNPRVGVATATQLQGKELSYNNINDTDAAFELIAEFDPAQGPAVAIIKHANPCGV
                     AVGGSQREAYERALACDPTSAFGGIVAVNSRLTREAALQMVEIFTEVVIAPEADDDAI
                     AVFAAKKNLRLLVTGGLPDALSTGDTFKSVAGGFLVQSRDDARIKAADLKIVTRRQPT
                     DEEVRDMLFAFTVGKHVKSNAIVYARAGQTLGVGAGQMNRKDSARIAALRAADFGLDL
                     KGCACASEAFFPFADGLIQAAEAGATAIIQPGGSMRDGEVIEAADRLGLTMAFTGVRV
                     FRH"
BASE COUNT          294 a          643 c          621 g          280 t
ORIGIN      
        1 cagccaggcc agcaccgcaa acaggtcggc cgcgcgcttc aggccatgca gccgcacgcc
       61 ggtgcagacc agcagcttgg cgtccagggt cgacatcccg cgcggcttca gcgagtcgac
      121 cacgatggcc gcgtagcccg cgtccaccgc cgtctgggca taggcctcga gaaacggggt
      181 cttgccgccg cagccgtgca ggatcagcgc caccggaaag gggccgggcc ccacgggctt
      241 gaacacctgc gaggcgtcga cgaccttggc tccccaggcg tggctttgca tcatccgggc
      301 cctaatgcct gaaaacccgc acgccggtga aggccatggt caggcccagc cggtcggccg
      361 cctcgatcac ctcgccatcg cgcatcgagc cgccaggctg gatgatcgcc gtcgccccgg
      421 cctctgccgc ctggatcaga ccatccgcaa acggaaagaa ggcttccgag gcgcaggcgc
      481 agcccttcag gtccaagcca aaatcagcgg cccgaagggc cgcgatccgg gccgagtcct
      541 tgcggttcat ctgaccggcg cccacgccca gggtctgacc agcgcgggca tagacgatgg
      601 cgttggactt gacgtgcttg ccgacggtga aggcgaacag catgtcgcgc acctcttcgt
      661 ccgtaggctg acgtctggtg acgatcttca ggtcggccgc cttgatccgc gcgtcgtcgc
      721 gcgactgcac cagaaaaccg ccagccacgc tcttgaaggt atcgccggtc gacagggcgt
      781 cgggcaggcc gccggtcacc agcaggcgca ggttcttctt ggcggcaaac acggcgatgg
      841 cgtcgtcatc ggcttccggt gcgatcacca cctcggtgaa gatctcgacc atttgcaggg
      901 cggcttcacg ggtcaggcgg ctattgaccg ccacgatgcc gccgaaggcc gaggtggggt
      961 cgcaggccag cgcccgctca taggcctcgc gctggctgcc gcccacggcc acgccgcacg
     1021 ggttggcgtg cttgatgatg gccacggccg gaccctgggc cgggtcgaac tcggcgatca
     1081 gctcgaaggc cgcgtcggtg tcgttgatgt tgttgtagct gagctccttg ccctgcagct
     1141 gggtggccgt ggccaccccg acgcggggat tggggaacgc atagaaggcc gccttctggt
     1201 gcgggttctc gccatagcgc atcacctggc gcagctcgcc ggcgatggcc ttgcgggccg
     1261 ggaagtcctg gcccagcgcg gccgcgaacc aggccgagat cgccgcgtca taggccgccg
     1321 tgcgggcata ggcgcgggcc gccagctgct ggcggagtcc gaacgaagtc gcgccgccgg
     1381 ccttcagggc ttccagcact tcggccacat cggcaggatc ggtgcagacc gccacatagc
     1441 cgtggttctt ggccgccgag cggatcatgg ccgggccgcc gatgtcgatg ttctcgacgc
     1501 actcggcgta gctgccgccc ttagccaccg tggcctcgaa cggatagaga ttcacataca
     1561 ggatatcgat ggcctcgacg ccatgctcga ccatggcggc gacatgctcc ggcgcgtcgc
     1621 gaacacccag cagcccgccg tgaacgaggg ggtgcagggt cttgacccgg ccgtccatca
     1681 tctcggggaa gcccgtcagg tccgagacgt ctttcaccgg caggccggcc gccgcgatgg
     1741 ccgccttggt gccgcccgtc gagaccagct cgacgccggc ctcgtgcagc acccgggccg
     1801 cctcgaccag gccggtcttg tcggagaccg acagcagg
//
LOCUS       DAOMEO010000699         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6585_length_1837_cov_1.994949, whole genome shotgun sequence.
ACCESSION   DAOMEO010000699 DAOMEO010000000
VERSION     DAOMEO010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_1837_cov_1.994949"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /gene="fabA"
                     /locus_tag="PLH31_14235"
     CDS             complement(<1..394)
                     /gene="fabA"
                     /locus_tag="PLH31_14235"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HQR90390.1"
                     /translation="MQQSSYTYDELLACGRGEMFGPGNAQLPAPPMLMFDRIVRIEAE
                     GGKHGKGYVEAEFDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFFLGWSGGPGRG
                     RALGVGEVKFTGQVTQDVKKVVYKIDLKR"
     gene            complement(489..1013)
                     /locus_tag="PLH31_14240"
     CDS             complement(489..1013)
                     /locus_tag="PLH31_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQR90391.1"
                     /translation="MPSNCKHRSFGIVAALALAGFASSAGAADPPRVTPSGLEVPRYV
                     SLKYEKVNARNGPDEAHRLLWVYRAKGLPVQVVAETREWRRICDPEGGLAWVHKRTID
                     GRRTAMRLQPQPLALLVNPKEGARISAWLRGRSTASLDKCEKGWCRLKAEGSSGWARE
                     GGIWGASPQAQCKP"
     gene            1087..>1837
                     /locus_tag="PLH31_14245"
     CDS             1087..>1837
                     /locus_tag="PLH31_14245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HQR90392.1"
                     /translation="MAARKLKVIVTRKLPDPVETRMCELFDTELNVTDKPLTADELID
                     AMGRADVLVPTITDRIDSRLLSRSGDRLKLIANFGAGVDNIDVATANARGIIVTNTPG
                     VLTEDTADLTMTLIMAASRRVVEGAEVVKSGGFHGWSPTWMLGRRLWGKRLGIIGMGR
                     IGQAVARRAKAFGMQVHYHNRKPVSPRIAEELGCTYWESLDQMLARMDFISVNCPHTP
                     ATYHLLSARRLKLLRPHAIVVNTARGEVIDEG"
BASE COUNT          314 a          643 c          575 g          305 t
ORIGIN      
        1 cccgcttcag gtcgatcttg tagaccacct tcttgacgtc ctgggtgacc tggccggtga
       61 atttcacttc gccgacgccc agggcgcggc cgcggccggg accgccagac cagcccagga
      121 agaagccgac cagctgccac atggcgtcca ggcccaggca gcccggcatc accgggtcgc
      181 cgataaagtg gcagccgaag aaccagagat cgggattgat gtcgaattca gcctcgacat
      241 agcccttgcc gtgcttgcca ccctcggcct cgatccggac gatgcggtcg aacatcagca
      301 tgggcggcgc cggcagctgc gcgttgccgg ggccgaacat ctcgccccga ccgcaggcca
      361 ggagttcgtc gtaggtgtag gagctctgtt gcatggtcac gccctagcac gacggtgggt
      421 cgcgcctcaa tgttactttc agtaacgctt gtgaacgctt cagggtcctt cgtcacgccc
      481 cttgccggtc agggtttgca ctgggcctgc ggagacgcgc cccagatccc gccctcgcgc
      541 gcccaaccgg acgatccctc ggccttcagt cggcaccagc ctttttcgca cttgtcgagg
      601 ctggcggtcg agcgaccccg cagccaggcg ctgatcctgg cgccctcctt ggggttgacc
      661 agcagggcca gcggctgggg ctggaggcgc atggcggtgc ggcgaccatc tatggtgcgc
      721 ttgtgcaccc aggccaggcc gccttccgga tcgcagatcc gccgccattc gcgggtttcg
      781 gccaccacct gcaccggcag gcccttggcg cgatagaccc acagcagacg atgagcctcg
      841 tccggaccat tgcgggcgtt gaccttttca tacttcaggg agacgtagcg cggcacctcg
      901 aggcctgagg gcgtcacgcg cggcggatcg gcggcgcccg cgcttgaggc gaaccccgcc
      961 agggccagcg cggcgacgat accgaacgaa cgatgtttgc aattcgacgg catgttgctt
     1021 ggccccatcg ggcccctttg ctacaggttt tcaaacgccc gtcagagaga gcttttcaaa
     1081 ccgcccatgg cagcccgcaa gctcaaagtc atcgtcaccc gcaaactccc cgatccggtg
     1141 gagacgcgca tgtgcgagct gttcgacacc gaactcaacg tcaccgacaa accgttgacg
     1201 gcggatgaac tcatcgatgc gatggggcgg gccgatgtgc tcgtgccgac gatcaccgat
     1261 cggatcgact cgcgccttct gtcgcgatcc ggcgatcgac tcaagctgat cgccaatttc
     1321 ggggccggag tcgacaatat cgacgtcgcc acggccaatg cgcgcggcat catcgtcacc
     1381 aacacgcccg gcgtgcttac cgaagacacc gccgacctga ccatgaccct gatcatggcc
     1441 gcctcgcgcc gcgtcgtcga aggcgccgag gtggtcaaga gcggcggctt ccatggctgg
     1501 tcgcccacct ggatgctggg ccgccgcctg tggggcaagc gcctgggcat catcggcatg
     1561 ggccggatcg gccaggccgt ggcccgccgc gccaaggcct tcggcatgca ggtgcactat
     1621 cacaaccgca agcccgtcag cccgcgcatc gccgaggagc tgggctgcac ctactgggag
     1681 agcctcgacc agatgctggc ccggatggac ttcatctcgg tcaactgccc gcacacgccc
     1741 gccacctatc acctgctgtc ggcccgccgc ctgaagctgc tgcggccgca cgccatcgtg
     1801 gtcaacaccg cccgcggcga ggtcatcgat gaaggcg
//
LOCUS       DAOMEO010000700         1834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6593_length_1834_cov_2.225408, whole genome shotgun sequence.
ACCESSION   DAOMEO010000700 DAOMEO010000000
VERSION     DAOMEO010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_1834_cov_2.225408"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..457)
                     /locus_tag="PLH31_14250"
     CDS             complement(305..457)
                     /locus_tag="PLH31_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90393.1"
                     /translation="MTTTPPEDTTPEIETPHVAKVGVKDTGHNVTSAADEPINGLLAD
                     IADGDV"
     gene            complement(763..1599)
                     /locus_tag="PLH31_14255"
     CDS             complement(763..1599)
                     /locus_tag="PLH31_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90394.1"
                     /translation="MSSGFVSAQDGVQIFYKDWGPKSAQPIVFHHGWPLSSDDWDAQM
                     MFFLLKGYRVIAHDRRGHGRSTQTDTGNEMDTYAADVIALANHLDLKNAFHVGHSTGG
                     GEAIHYAARAKPGRVSKVVLVGAVPPIMLKTPANPGGLPMEVFDGFRADLVANRAQFF
                     RDIPEGPFFGFNRPGAKVSQGLIDNWWRQGMEGGAKAHYDCIKAFSETDFTDDLKKVD
                     IPVLIMHGEDDQLVPIANSAHLAIKLVKKGTLKTYPGLPHGMASTHPEIINADLLAFF
                     KA"
BASE COUNT          285 a          544 c          676 g          329 t
ORIGIN      
        1 cggcgacggc gacccgggtc tcggccgccg gggcgcagcc tcccgccagc agcgcgaacg
       61 cggcggcgag cggggcggcc agcaacgggc ggcgggcgat catgcgagac ctcggcggct
      121 tgggaggcgg cgtcaaaaac ccaccgtctg tagcaagacc ccctagcgct gtccccctcg
      181 catccggggc gaccccgcgc agcgctcgtc tggcgccagg gcgctggggc cggatgggca
      241 atcgcggtct aagggggttg atttgaggcg ccgtcacgac cctggccgcg acggcgcgca
      301 ggaatcagac gtcgccgtcg gcgatgtcgg ccagcaggcc attgatcggc tcatcggccg
      361 cgctggtgac attgtggccg gtgtctttga cgccaacctt ggcgacatgc ggggtttcga
      421 tttcgggcgt ggtgtcttcg ggaggcgtgg tggtcatcgg gtagcctttg gatgcagggg
      481 cgtcgggcga ctgtcgcgcg acggttccaa gccaacggtg tcatggctac gccaggctgt
      541 caggtaaact caacttcgca tgagcgccaa gccgccgacc ggcgcgaact tgggcgttcg
      601 ccggggctct tcgcacggtg ggggcgtagg ccggtcccaa aactcttggg ggcgcaggac
      661 accaatgtcc aaggaactcc aggaaccgcc gccacacgcg gaggcgtggc gctgccgatt
      721 ggcttgcttt gcttctcgca tgacgacggt ccccaacgat ccctaggcct tgaagaacgc
      781 cagcaggtcg gcgttgatga tctcggggtg ggtggaggcc atgccgtgcg gcaggccagg
      841 ataggtcttc agcgtgccct tcttgaccag cttgatggcg agatgagcgc tgttggcgat
      901 cgggacgagc tggtcgtcct cgccgtgcat gatcaggacc gggatatcga ccttcttgag
      961 gtcgtcggtg aagtcggttt ccgagaaggc cttgatgcag tcgtagtggg ccttggcgcc
     1021 gccttccatg ccttggcgcc accagttgtc gatcaggccc tggctgacct tggcgcccgg
     1081 acggttgaaa ccgaagaacg ggccttcggg gatgtcgcgg aagaactggg cgcggttggc
     1141 gaccaggtcg gcgcggaagc cgtcgaacac ttccatgggc agtccgccgg gattggccgg
     1201 ggtcttcagc atgatcggcg gcaccgcgcc gaccagcacg accttgctca cccggcccgg
     1261 cttggcgcgg gcggcgtagt ggatggcctc gccgccgccg gtcgagtggc cgacgtggaa
     1321 ggcgttcttg aggtccagat ggttggccaa ggcgatgacg tcggcggcgt aggtgtccat
     1381 ttcgttgccg gtgtcggtct gggtcgagcg gccatggccg cggcggtcat gggcgatgac
     1441 gcgatagccc ttgagcagga agaacatcat ctgggcgtcc cagtcgtccg acgacagcgg
     1501 ccagccatgg tgaaacacga tcggctgggc gctcttggga ccccagtcct tgtagaagat
     1561 ctggacgccg tcctgggcgc tgacgaaacc gctgctcatg gtggaagatc ctttcgtggc
     1621 ttgggaaggg gcggcctggg ccgaggcggg aagggcgggg atgagcgacg gcgcgacgct
     1681 gattgccgcc agactcagac ccgtggcaag gacgccgcga cgcgaggcgt gcgggccatc
     1741 taacgcattg ttgttcatgg aaaatatcct ctgcggtcga ctggcggttt gccggtcttg
     1801 acctagtggc tgtggatgct cgtacgagtt cgct
//
LOCUS       DAOMEO010000701         1833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6599_length_1833_cov_2.185039, whole genome shotgun sequence.
ACCESSION   DAOMEO010000701 DAOMEO010000000
VERSION     DAOMEO010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_1833_cov_2.185039"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLH31_14260"
     CDS             complement(<1..1288)
                     /locus_tag="PLH31_14260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90395.1"
                     /translation="MSETFAAAPAKAKATHYRWLVLAVLTAVHSTHHIDRNVLSVVVE
                     PIRQEFGLSDGQMGMLGSLGYALAFAVAAIPMGYLVDRVNRRNMLVGILALWSVMTAV
                     CASANSYVHLLLARMGVGIAESGGAPTAMSMVSDYFPPKQRSTAIGIWYLSSAIGTGI
                     IFLVGGFLAQSFGWRTVFLVAGIPGLVMGAILFLVVREPRRGASEVLALDIPEASPAA
                     AVDMPEKAATPREAFAYVIARPAILSMMAGIVLAAAMSSAFALWSVSFLVRVHHMPLA
                     LAGVSIAAAFSVFGIVIPLISGLMGDRLSNSKHGHRPERLALLSATTMTGVVICGVAA
                     ALSGSAPVAVAMMCLWCGLMLAHNGPANALVLGLLRPRMRGVVVATLQTVATVVGTGL
                     GPFLVGVLSDVYGGPNSLRWAIMTGMSLNVLAVLCFL"
     gene            complement(1288..1821)
                     /locus_tag="PLH31_14265"
                     /pseudo
     CDS             complement(1288..1821)
                     /locus_tag="PLH31_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          351 a          603 c          623 g          256 t
ORIGIN      
        1 tgaggaagca cagcaccgcc aagacgttca gcgacatgcc ggtcatgatg gcccagcgca
       61 gcgagtttgg accgccatag acgtcgctga gcacccccac cagaaagggg cccagccccg
      121 tgccgacgac cgtcgcgacg gtctgcagcg tggcgacgac gacgccgcgc atccgagggc
      181 gaagcaggcc gaggaccagg gcgttggccg gcccgttgtg cgccagcatt aggccgcacc
      241 acaggcacat catcgccacc gcgaccggcg cgctgccgga cagggcggcc gcgacgccgc
      301 agatgacgac cccggtcatg gtcgtggcgc tgagcagggc caggcgctcg ggcctgtggc
      361 cgtgcttcga gttcgacaac cggtcgccca tcaggccgga gatcaacgga atgacgatgc
      421 cgaacaccga aaaggcggcg gcgatcgaaa ctccggccag ggccagcggc atgtgatgaa
      481 cccgcacaag gaacgacacc gaccacaggg cgaaggccga gctcatcgcc gcggccagga
      541 cgatgccggc catcatgctg agaatagccg ggcgagcgat cacataggcg aaggcttcgc
      601 gaggggtggc ggccttttcc ggcatatcga cggccgcggc ggggctggct tcgggaatgt
      661 cgagggccaa gacttccgat gcgccgcgcc gaggctcgcg cacgaccaga aacaggatcg
      721 cccccatcac cagcccgggg atacccgcga ccaggaatac cgtgcgccag ccgaacgact
      781 gggccagaaa gccgccgacc aggaagatga tcccggtccc gatcgccgaa ctcaggtacc
      841 agatgccgat cgccgtcgag cgctgcttgg gcgggaaata gtccgagacc atcgacatgg
      901 cggtcggagc gccgcccgac tccgcgatgc cgacgcccat ccgggccagc agcagatgga
      961 cataggagtt ggccgaggcg cagacggccg tcatcacgct ccacagggcc aggatgccga
     1021 ccaacatatt gcggcggttc actcggtcga cgagatagcc catcgggatc gcggcgacgg
     1081 cgaaggccag ggcgtagccc aggctgccca gcatccccat ctggccgtcg ctgaggccaa
     1141 actcctgccg gattggctcg acgacgaccg acaagacgtt gcggtcgatg tggtgggtcg
     1201 aatgcacggc ggtcagcacc gccagcacga gccagcgata gtgggttgcc ttggcctttg
     1261 cgggcgcggc cgcaaatgtc tccgacatca gccgagccct tcgagatatt gcgccagatc
     1321 gtggctgtgg ggttgatggg cgagggagcc gccgttgcag acgatggtct cgccattgat
     1381 gaaacccgcg tcctcggacg ccaggaaggc gaccacagcc gccacatcct cgggcgaccc
     1441 gagacgcggc gtcaggacat ggttctagat catctcgatc agttccggca cgcccgcctt
     1501 cgaggcctcg gtgagaatca ggcccggcgc gacggcgttg caccggacac cctggcgacc
     1561 gtactgggtc gcgatgaagc gggtcagggc gatgactccg gccttggacg tgccgtagcc
     1621 gatccgcgac aggtcgccga ccaatccgga tccggaggcc gtgttgatga tcgcgccgcc
     1681 gccgaccttc agcatttcgg gaatggcgta tttgcacccg gccagatagc cgcgcaggtt
     1741 gaccgccatc acccggtccc aggtctcgaa ggtgatgtcg accgccgtgg tgtcttcacg
     1801 ctggatctgc ggcgtggtga tcgcggcgtt gtt
//
LOCUS       DAOMEO010000702         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6618_length_1828_cov_1.969543, whole genome shotgun sequence.
ACCESSION   DAOMEO010000702 DAOMEO010000000
VERSION     DAOMEO010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_1828_cov_1.969543"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>851)
                     /gene="nhaA"
                     /locus_tag="PLH31_14270"
     CDS             complement(<1..>851)
                     /gene="nhaA"
                     /locus_tag="PLH31_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQR90396.1"
                     /translation="VFFLLVGLEIKREVLDGQLSKPSRVILPGVAALGGVVVPALIYL
                     AFNAGSPASRDGWAIPSATDIAFALGVLALLGPRVPGSLKIFLTAIAIMDDLAAIVII
                     ALFYTAQLHVAYLAGAGCVLIGLFVLNRMKVMTLWPYLLGGLVLWWLVLESGVHATLA
                     GVALAMLIPLRKSPAAPDHVASPLHRLEHSLHKPVAFLIVPLFGFANAGLSFVGVSPA
                     ALVDPVPLGVALGLFVGKQVGVFLTAIAIVRLGWADMPRDATATQLYGVSVLCGIGFT
                     MSLFIGN"
     assembly_gap    852..861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<862..1088)
                     /locus_tag="PLH31_14275"
     CDS             complement(<862..1088)
                     /locus_tag="PLH31_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQR90397.1"
                     /translation="MTPTASRSRPLSALRAFLYHEASGGYVLMVAAALALVIANSPLA
                     AAYFAALKTEFGFTIGAFHLRESVIHWINDGL"
     gene            complement(1345..>1828)
                     /locus_tag="PLH31_14280"
     CDS             complement(1345..>1828)
                     /locus_tag="PLH31_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HQR90398.1"
                     /translation="GDAAFSERFFKTTIKGAELPDFEPLLARAGLKLRRKAPKRAWLG
                     ALRIRVNGGEVLLAEPPAPNTPLYVAGVESGDRIVGIGRFEFASEADWLDALERLKPD
                     EATSVRFIQRGQVREAPLKVAADPSLEIIRYETANLKPTKAQLTFRDQWLGADELPAK
                     "
BASE COUNT          368 a          561 c          612 g          277 t
ORIGIN      
        1 ggttgccgat gaacaggctc atggtgaagc cgatgccgca gagcacggac acgccgtaga
       61 gctgtgtcgc tgtggcgtct ctgggcatgt cggcccagcc caggcgcacg atggctatgg
      121 ccgtcaggaa gacgccgacc tgcttgccga caaagaggcc cagcgccacg ccgaggggca
      181 cgggatcgac cagggcggct ggcgaaacgc cgacgaagga caggccggca ttggcgaaac
      241 cgaacagggg cacgatcagg aaggcgactg gcttgtgcag ggaatgttcg agacggtgca
      301 gcggcgatgc gacgtgatcg ggcgctgcag ggctcttgcg cagcgggatc aacatggcca
      361 gggctacgcc cgccagggtc gcgtggacgc ccgactccag aacaagccac cacaggacga
      421 gaccgcccag cagatagggc cacagcgtca tcaccttcat ccgattgagg acgaacaggc
      481 cgatcagcac gcagcccgcg cccgccagat aggccacgtg aagttgcgcg gtgtagaaca
      541 gtgcgatgat cacgatcgcg gcaaggtcgt ccatgatggc gatcgccgtc aggaagatct
      601 tcagggatcc gggaacccgg ggtcccagta gcgccaggac gcccagggcg aaagcgatgt
      661 ccgtagccga agggatggcc cagccgtcgc gactggccgg ggagccggca ttgaaggcca
      721 gataaatcag cgccgggacg accacgccgc ccagggccgc gacgcccggt agaatgacgc
      781 gcgacggctt ggagagctgg ccgtcgagga cctcgcgttt gatctccaga ccgacgagca
      841 ggaagaacac cnnnnnnnnn naggccgtca ttgatccagt ggatcacact ctcgcgcaga
      901 tggaaggcgc caatcgtaaa gccgaactct gtcttcagcg ccgcgaagta ggcggcagcc
      961 aacggagagt tggcgatcac cagggcgagc gccgcagcca ccatcaacac gtagccgccc
     1021 gaggcctcgt gatacaggaa ggcgcgaagg gccgagaggg ggcgggaccg ggatgcggtt
     1081 ggcgtcatgc aggtcacatc tttctaacgg gcgtcgcgct gacgcaggag gggagagcgc
     1141 cgccccgacc ggaggcacgc gccgtcgcgc gtcaagttcg tgatctggcc cagggggtca
     1201 agtctaaccg ccgtattggc tcagagattt ggtcggtgtc gttctgaagg caatagacgc
     1261 agggatggat gccgcgccga ctgcttaaat gaccaggctg agaggcgctg ttcgcctccg
     1321 ccgcccgcca ctcccaaccc ctgcctactt agccgggagc tcatcggctc ccagccattg
     1381 atcgcgaaat gtcagttggg ccttggtggg ctttaggttg gcggtctcat accggatgat
     1441 ctctaaggac gggtcggctg cgactttcag cggcgcttca cggacttgcc cccgctgaat
     1501 aaaccgcaca gaggtcgctt catccggctt tagcctttcc agcgcatcca gccagtctgc
     1561 ctcgctagcg aactcgaacc ggccaatccc gacaatgcga tcgccggact caacgccagc
     1621 cacatagagg ggggtgttcg gcgcaggcgg ttccgccagt aggacttcgc cgccgttcac
     1681 gcggatccgg agagcgccca gccaggcgcg cttgggtgct tttcggcgaa gtttcagtcc
     1741 agcacgggcc agtagcggct cgaagtcagg cagttctgcc cccttgatgg tggtcttgaa
     1801 gaagcgctcc gagaaggccg catctccc
//
LOCUS       DAOMEO010000703         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6619_length_1828_cov_1.957699, whole genome shotgun sequence.
ACCESSION   DAOMEO010000703 DAOMEO010000000
VERSION     DAOMEO010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_1828_cov_1.957699"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>240
                     /locus_tag="PLH31_14285"
     CDS             <1..>240
                     /locus_tag="PLH31_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90399.1"
                     /translation="TQAKIDEVVGIYNSGYASDFAAGSILRTTPVLDEKYSAKIDWNI
                     NDQHRASLSYRRAESAFTSRANLGTFTAALDSQWYD"
     assembly_gap    241..250
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <251..>1828
                     /locus_tag="PLH31_14290"
     CDS             <251..>1828
                     /locus_tag="PLH31_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90400.1"
                     /translation="TTLEINSDWTDRLSTTVRATYRDWVKGQMPKSGQNYSDVTICTA
                     PTADAAPTNSSITTCATGNSSVRFGPDQFRHANQLDIQEAVFQGTATYSLDDHLFKFG
                     YQGSKKEVFNVFVPQSRGVYYFDSVADFAAGRASSLTYNNSVTGNAIDAAADFKYWQH
                     SLYAQDAIDVTDDIQVTAGFRFDWIDMNDVPTENPNFTARNGFANTSTLDGLSILMPR
                     VSASWRATDTLKLTAGFGLFSGGQPEVLFATPFYNNGFQTAGTTIRRCITAASNCTGA
                     NGYLEASSSASVTPGFNAAVGAAALDNLNTFSGFGYEIPTLVKSFQEGTLAGTPGISP
                     NNEVIALNPAFQLPAEWKLYFGANWEVYDGWNLDVQYVATQAQDAVTFVDARAQPLVI
                     NGQRALTPDGRPRYDGLTLSAAQRAAQGVSSINPGDNRDLITNNVDKGQAFTAGVTLS
                     KSFDFGLDLGLGYAKQNIDESNAGLRFGTTAGSLYNSVPAGLDPARDAYGTALEEIKD
                     RYKLETGYRKKFFGDNET"
BASE COUNT          354 a          658 c          510 g          296 t
ORIGIN      
        1 acccaggcga agatcgacga ggtcgtcggc atctacaaca gcggctatgc gagcgacttc
       61 gctgcgggct cgatcctgcg gaccacgccg gtcctcgacg agaagtattc ggccaagatc
      121 gactggaaca tcaacgacca gcatcgcgcc agcctcagct atcgccgcgc ggaatcggcc
      181 ttcacctcgc gcgccaacct cggcaccttc acggcggccc tggactccca gtggtacgac
      241 nnnnnnnnnn caccacgctg gaaatcaact cggactggac cgatcgtctg tcgacgaccg
      301 tccgggccac ctatcgtgac tgggtcaagg gccagatgcc caagagcggt cagaactatt
      361 cggacgtgac catctgtacg gcccccaccg ccgacgccgc tccgaccaat tcgagcatca
      421 ccacctgcgc caccgggaac tcgtcggtcc gcttcggacc cgaccagttc cgtcacgcca
      481 accagttgga catccaggaa gcggtgttcc agggcacggc gacctattcg ctggacgacc
      541 acctcttcaa gttcggctat cagggctcga agaaggaagt gttcaacgtg ttcgtgccgc
      601 agtcgcgcgg cgtgtactac tttgactcgg tcgccgactt cgcggccggt cgcgccagct
      661 cgctgaccta caacaactcg gtcaccggca acgccatcga cgccgccgcc gacttcaaat
      721 actggcagca ctcgctgtac gcgcaggacg ccatcgacgt caccgacgat atccaggtca
      781 ccgcgggctt ccgctttgac tggatcgaca tgaatgacgt tccgaccgaa aaccccaact
      841 tcacggcgcg caacggcttt gccaacacca gcaccctgga cggcctgagc atcctgatgc
      901 cgcgcgtctc ggcctcctgg cgcgcgaccg acaccctgaa gctgacggca ggctttggcc
      961 tgttctcggg cggccagccg gaagtgctgt tcgccacgcc gttctataac aacggcttcc
     1021 aaaccgccgg caccaccatc cgccgctgca tcaccgccgc gtcgaactgc acgggcgcca
     1081 acggttatct ggaagcctcg tcgtcggcgt cggtcacccc cggcttcaac gccgcggtcg
     1141 gcgccgccgc cctggacaac ctgaacacct tctcgggctt cggttacgaa atcccgaccc
     1201 tggtgaagtc gttccaggaa ggcaccctgg ccggcacccc gggtatcagc ccgaataacg
     1261 aagtcatcgc cctgaacccg gccttccaac tgccggcaga atggaagctg tacttcggcg
     1321 ccaactggga agtgtacgac ggctggaacc tggacgttca atatgtggcc acccaggccc
     1381 aggacgccgt gaccttcgtc gacgcccgcg cccagcctct ggtgatcaac ggccaacgcg
     1441 ccctgacccc cgacggtcgt ccgcgttatg acggtctgac tttgagcgcc gcccagcgcg
     1501 ccgcccaggg cgtgtcgtcg atcaaccccg gcgacaaccg cgacctgatc accaacaatg
     1561 ttgacaaggg tcaggccttc accgccggcg tcaccctgtc caagtccttc gacttcggtc
     1621 tggacctggg cctcggctac gccaagcaga acatcgacga gtcgaacgcc ggcctgcgct
     1681 tcggcaccac cgccggctcg ctgtacaaca gcgttccggc cggcctggat ccggcccgcg
     1741 acgcctatgg caccgcgctg gaagagatca aggaccgcta caagctggaa accggctacc
     1801 ggaagaagtt cttcggcgat aacgagac
//
LOCUS       DAOMEO010000704         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6630_length_1825_cov_1.997740, whole genome shotgun sequence.
ACCESSION   DAOMEO010000704 DAOMEO010000000
VERSION     DAOMEO010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6630_length_1825_cov_1.997740"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>408)
                     /locus_tag="PLH31_14295"
     CDS             complement(<1..>408)
                     /locus_tag="PLH31_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90401.1"
                     /translation="GTGPADGGELIVNSVEGQGATFILTLTVEAVDAPQAGEDGPSIT
                     GAQVLVVDDHVVNRRAIELVLQPFGIEATLAESGEQALELLGAKIFDVVLMDVYMPGM
                     DGREATRTLRAGAGLNRETPVIAVTASATPKDW"
     assembly_gap    409..418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<419..1517)
                     /locus_tag="PLH31_14300"
     CDS             complement(<419..1517)
                     /locus_tag="PLH31_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR90402.1"
                     /translation="MGSSETQRNLARAAASRRSNTYLRLAATLVIALILHVFIGMAWV
                     WAWAALYVLAQLFELWITSSVMASPEVRPPAWRRLALTVSPFITSAIFGFLSLPLFAS
                     HERFAPTLGGMLLAGALLNVIVVNGSLRSATLSAAAPHVIYLMIVPFIGRIANPDSPL
                     ANALWFGALLLVASVAVASRTLGAALKAEADAKEEAELRRHEAEEAVAAKSAFVAMIS
                     HELRTPISAILAGAARLHSEVPEASSKTHAQLITDAGGLMRALLNDLLDFSRLEVGRM
                     AVEKAPYNLRQTLSDTLRLWRPEVAKKGLRLRIEGAATLPRWVAGDALRLRQVLNNLL
                     SNAIKFTDQGAIAVRFDSREDGVQRRLTITVT"
     gene            complement(1730..>1825)
                     /locus_tag="PLH31_14305"
     CDS             complement(1730..>1825)
                     /locus_tag="PLH31_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HQR90403.1"
                     /translation="RLTAGATARLKRLEIDGVEEADLEKAFGPRP"
BASE COUNT          324 a          597 c          590 g          304 t
ORIGIN      
        1 cccagtcctt gggcgtcgcc gaagcggtga cggcgatcac aggcgtctcg cgattgaggc
       61 ccgcgccggc ccgcagggtg cgggtcgctt cccgaccgtc catgccgggc atgtagacgt
      121 ccatcagcac cacatcgaag atcttggcgc ccagcagttc cagggcctgc tcgccggact
      181 ccgccagggt ggcctcgatc ccgaagggct gcagcaccag ttcgatggcc cggcgattga
      241 ccacgtgatc gtcgaccacc agcacctgcg cgccagtaat ggagggaccg tcctcccctg
      301 cctgtggcgc atccaccgcc tcaaccgtca gggtcaggat gaatgtggcg ccctggccct
      361 cgacgctgtt gacgatcagc tcgcccccat cagccgggcc agttcccgnn nnnnnnnncc
      421 gtgacggtga tggtcagacg tcgctggact ccatcctcac gactgtcgaa ccgcaccgcg
      481 atggcgccct ggtcggtgaa cttgatggcg ttggacaaca gattgttaag cacctgacgc
      541 aggcgcagcg cgtcacccgc cacccatctg ggcagggtcg cggccccttc gatacgtagg
      601 cgaaggccct ttttggccac ctcggggcgc cacagccgca aggtgtccga cagggtctgg
      661 cgaagattgt agggcgcctt ttcgacagcc attcggccga cctccaggcg cgagaaatcc
      721 agcaggtcgt tgagcagggc gcgcatcagc ccgccagcgt cggtgatcag ttgggcgtgg
      781 gtctttgaac tggcctccgg gacctcgcta tgcagccgcg cggcgcccgc caggatggcg
      841 ctgatcgggg tgcggagctc gtggctgatc atggcgacaa aggccgactt ggcggccacg
      901 gcctcttccg cctcatgccg gcgaagctcg gcttcttcct tggcgtcggc ctccgccttc
      961 agcgccgcgc caagggtgcg gctggccacg gcaaccgatg cgaccagcaa cagggcgcca
     1021 aaccaaagag cattggccag cggcgagtcg gggttggcga tccgtccgat gaacgggacg
     1081 atcatcagat agatcacatg cggcgcggcg gctgacagcg tcgccgacct gaggctgcca
     1141 ttgacgacga tgacattcag cagcgcgccc gccagcaaca tgccgcccag cgtcggcgcg
     1201 aaccgctcgt gcgaggcaaa cagcggcaga gacaggaagc cgaagatggc cgaggtgatg
     1261 aacggcgaca cggtcaaagc cagccgccgc caggcgggag ggcgaacttc ggggctggcc
     1321 atgaccgaac tggtgatcca cagctcgaac agttgggcca ggacatagag cgccgcccag
     1381 gcccagaccc acgccatgcc tatgaagaca tgcaggatca gggcgatgac cagggtggcg
     1441 gccagacgca gataggtatt ggagcgccgc gaagccgcgg cgcgcgccag gttcctctgc
     1501 gtttcgctag accccatcgg cgtcctgtcc cctcgggttg acgcgtgcct cattttatcg
     1561 gtcagcttca cagccgaaac ctaagatgac gtgaagtatt ggcgttccgt caatggcttg
     1621 cgcgatgatg ttcggctctg ccgcatgcaa ttttcacagt gagccagacc cttagaggtt
     1681 ttgtccgtgg gaaatcttgc ccgatatggt gtccggtcgc gtttgacgct cagggccgcg
     1741 ggccaaaggc cttttcaaga tcggcctcct ccacgccgtc aatctccagg cgcttgaggc
     1801 gcgcggtcgc accggccgtc aggcg
//
LOCUS       DAOMEO010000705         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6635_length_1824_cov_1.899378, whole genome shotgun sequence.
ACCESSION   DAOMEO010000705 DAOMEO010000000
VERSION     DAOMEO010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6635_length_1824_cov_1.899378"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..584)
                     /locus_tag="PLH31_14310"
     CDS             complement(90..584)
                     /locus_tag="PLH31_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HQR90404.1"
                     /translation="MKHLLSTLGASLLLITGSALAHEPAKPATASLTSLSPAARVAAL
                     AVDGFHAALAAGDTEKALALLAPDVLVVEEGGAERSRAEYASHHLAADAAFTRAVPST
                     PLSRSGLAQGDLAYIVSESRTTGVYGGKPVDRLSAETMVLRQEPAGWKIVHIHWSSRD
                     AKPK"
     gene            complement(619..1686)
                     /locus_tag="PLH31_14315"
     CDS             complement(619..1686)
                     /locus_tag="PLH31_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HQR90405.1"
                     /translation="MNRLVLIGLFPLALAGPAMAQTMDHSAMPGMTMPATPAAKPAKP
                     EAAAHQHHARPATTTAPKPTTPAAGEADPHAGHDMPTGTPMDHSAMPGMEMGAPAAAG
                     TTIGDLEVPTGPPPAAPSDHAADRVFSPAAMAAARGQLRREHGGLSSSMVMANIVEWA
                     PQSGKDSYRWEGEAWFGGDIHRLVLKTEGEGVVGEGVDQAELQALYSRAVGPYFNLQA
                     GVRHDFKPHPTRTYATVGFEGLAPYWFEVSGAAFLSDRGDLSGRLEGSYDQRITQRLI
                     LQPRAEFNLAASNDAATGMGSGLSNAELGLRLRYEIRREFAPYIGVTYDRKFGKTADY
                     SRAAGEDVEDARLVFGLRAWF"
     gene            complement(1683..>1824)
                     /locus_tag="PLH31_14320"
     CDS             complement(1683..>1824)
                     /locus_tag="PLH31_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516388.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQR90406.1"
                     /translation="VLPGGKVSWDFTADPGDWAFHCHMLYHMHAGMFQVFSVRPLDGG
                     AA"
BASE COUNT          305 a          594 c          627 g          298 t
ORIGIN      
        1 cggctcccag cagcagggcg cgccgcgcga ggggcgagcg aaggatcaag cgggtcacag
       61 gtgggctccg gaaggttaga aacgatcgac tatttcggct tggcgtcgcg ggacgaccaa
      121 tggatatgga caatcttcca acccgccggc tcctggcgaa ggaccatggt ctcggccgac
      181 aggcggtcca ccggcttgcc gccgtagaca cccgtcgtgc ggctttcgct gacgatatag
      241 gccaggtcgc cttgggccag gcccgaccgt gacagcggcg tgctgggcac agcgcgggtg
      301 aaggccgcgt cggcggccag gtgatggctg gcatattcgg cccgcgatcg ctccgcgccg
      361 ccctcctcaa cgaccagaac gtcgggcgcc aacaaagcca gggctttttc ggtgtcgccc
      421 gccgccagcg ccgcatggaa gccgtcgacg gccaaggccg cgaccctggc cgccggggac
      481 aggctggtca aggatgcggt ggccggcttg gctggttcat gagccagcgc tgagccggtg
      541 atcagcaaca ggctggcgcc aagcgtggaa agtaggtgct tcattctcaa ggtctcctgc
      601 cgcacgcggc atattgggtc agaaccaggc ccgaaggccg aacaccaagc gagcatcctc
      661 aacatcctcg ccggcggcgc ggctgtagtc ggctgtcttt ccgaattttc ggtcataggt
      721 gacgccgata tagggggcga actcccggcg gatttcatag cgaagccgca ggcccagttc
      781 cgcgttcgac agacccgaac ccatcccggt cgctgcgtca ttcgacgcgg cgaggttgaa
      841 ctccgcgcgc ggctggagga tcaggcgctg ggtgatgcgc tggtcgtagg acccttctag
      901 ccgcccagag aggtcgcctc tgtcggacag gaaggccgca ccgctcacct cgaaccaata
      961 gggcgcaagg ccctcaaacc caacggtcgc gtaggtgcgg gtggggtggg gcttgaaatc
     1021 atgccgcacc cccgcctgca gattgaaata gggaccaacc gcgcgggaat agagcgcttg
     1081 cagttcggct tgatcaacgc cttcaccgac gacgccttcg ccttctgtct ttagcactag
     1141 ccgatggatg tcgccgccga accaggcctc gccttcccag cgatagctat ccttgcccga
     1201 ctggggcgcc cactcgacga tgttggccat gaccatggaa ctgctcaagc cgccgtgctc
     1261 gcgccgaagt tggccgcgcg cggcggccat ggcggccggc gagaacacgc ggtcagcggc
     1321 gtggtcgctt ggcgccgccg gcggcggccc cgttgggacc tccagatcgc cgatcgttgt
     1381 gccagccgcc gctggcgccc ccatctccat gcccggcatg gcggaatggt ccatcggcgt
     1441 tcccgtcggc atgtcatggc cggcgtgcgg atcggcttcg ccggccgcag gcgtcgttgg
     1501 cttcggcgcg gtcgttgtcg cggggcgcgc atgatgctgg tgagccgccg cctctggctt
     1561 ggcgggtttc gcggccggcg tggcgggcat ggtcatgccc ggcatagcgg aatggtccat
     1621 ggtctgagcc atggccggcc cagccagggc gagcgggaaa aggccaatca ggacgagccg
     1681 gttcatgctg cgcctccatc gagaggccgc acgctgaaca cctgaaacat gccagcgtgc
     1741 atgtggtaga gcatgtggca gtggaaagcc cagtcgccgg gatcggcggt gaagtcccag
     1801 gacaccttgc ctcccggcag cacg
//
LOCUS       DAOMEO010000706         1823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6638_length_1823_cov_2.626697, whole genome shotgun sequence.
ACCESSION   DAOMEO010000706 DAOMEO010000000
VERSION     DAOMEO010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_1823_cov_2.626697"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..306
                     /locus_tag="PLH31_14325"
     CDS             58..306
                     /locus_tag="PLH31_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90407.1"
                     /translation="MTDEHDLVGFCCEELEDAVSSDPTDALIEHDSGLILLNLGERTE
                     DGETGVVLATIRFCPFCETEIQTDEDVEAALGDVEIVE"
     gene            306..566
                     /locus_tag="PLH31_14330"
     CDS             306..566
                     /locus_tag="PLH31_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HQR90408.1"
                     /translation="MAKLHFSLVAPERELFSGEVDMVQAPGAEGDFGVLANHAPFMTT
                     LREGRVTVKDGATTKLFDIQGGFADVGPDGLTILAEHAVEAA"
     gene            770..1459
                     /locus_tag="PLH31_14335"
     CDS             770..1459
                     /locus_tag="PLH31_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90409.1"
                     /translation="MRLALASLIALGVVSTAAVAQEQTAPAAAPAAPAQEAPAPAPVA
                     PADPNVPVVPAPPAAAPAETYVEPVRPAPSTDPVSLQLLSVLDRICKPAVVGGDFNAL
                     AKAAGLKLKKKMWTLDLGKPYQIVLDNPGSNTNVCTVTIQYAQGDAQAQALGTALHDW
                     ATWENSPQLRLIRNDQINGSDFRRLTVSWDDAWANGKAGLVYVRLKKTDGSPVGKKFE
                     QAQILYSTTSR"
     gene            1578..>1823
                     /locus_tag="PLH31_14340"
     CDS             1578..>1823
                     /locus_tag="PLH31_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HQR90410.1"
                     /translation="MAWIILFIAGLFEIGWAVGLKFTEGFTKPIPIALTAISLVLSMG
                     LLGWAVKTLPLGTAYAVWTGIGAVGTAIVGIVVFKEPA"
BASE COUNT          315 a          593 c          593 g          322 t
ORIGIN      
        1 ggtcggcccg atcgaggaag ccgtggccaa ggccgaaaag ctggcggcgg aagcgtaatg
       61 accgacgaac acgatctggt cggcttctgc tgcgaggagc tggaggacgc ggtctcttcg
      121 gacccgaccg acgcgctcat cgagcatgac agcggcctga tcctgctcaa tcttggcgaa
      181 cggacggaag acggggagac tggcgtcgtg ctggccacca tccgcttctg cccgttctgc
      241 gagaccgaga tccagaccga cgaagacgtc gaggcggccc tcggtgacgt ggagatcgtg
      301 gaataatggc aaagctgcac ttctctctgg tcgctccgga acgcgaactg ttctcgggcg
      361 aggtcgacat ggttcaggct ccgggcgccg aaggcgactt cggcgtgctg gccaaccatg
      421 cgcccttcat gacgacgctg cgcgaaggcc gcgtcaccgt gaaggacggc gcgaccacca
      481 agctgttcga catccagggc ggcttcgccg acgtgggccc cgacggcctg acgatcctgg
      541 ccgagcacgc ggtcgaagcg gcctaaacgc ttcggacgga ttcgaggaaa cgcctccgcc
      601 ctgctcaggg cggaggcgtt ttcgcgtctg gggtgaacct gcgcgacgta tcgacgcagg
      661 aaattacact tgtggcccaa tctccgtcgc attctgtcgc cataagggtt gcgagggacc
      721 gtctgtcggt tttaatgccg acctcttgcc tgatggggat atctcgataa tgcgcctcgc
      781 gctcgccagc ctgatcgccc tcggtgtggt ttcgaccgct gccgtcgccc aggagcaaac
      841 cgctcccgcc gccgcgcctg ccgcgcccgc acaggaagct ccggccccgg ctccggttgc
      901 tccagccgat ccgaatgtgc cggtcgttcc ggctccgcct gccgcggctc ccgccgagac
      961 ctatgtcgaa ccggtgcgcc cggcgccctc gacggacccg gtttcgctgc agctgctgag
     1021 cgtgcttgac cgcatctgca agccggccgt ggtgggcggc gacttcaacg ccctggccaa
     1081 ggcggccggc ctgaagctga agaagaagat gtggaccctg gacctgggca agccctacca
     1141 gatcgtcctc gacaacccgg gttcgaacac caacgtctgc acggtcacca tccagtacgc
     1201 ccagggcgac gcccaggctc aagccttggg caccgcgctc cacgattggg ccacctggga
     1261 gaacagcccg cagctgcgcc tgatccgcaa cgaccagatc aacggcagcg atttccgtcg
     1321 cctgacggtt tcctgggatg atgcctgggc caatggcaag gccggtctgg tctatgtgcg
     1381 tctgaagaag actgacggct cgccggtggg caagaagttc gaacaggccc agatcctgta
     1441 cagcaccacc agccgctagg acgtagcggg aggcttgtcc tcccgccatg ctgtcccata
     1501 tcaacgggag gcgcggggac gaccccgcgc cttttgtttt gtccggggac gaccccacta
     1561 gaaaagtgga gcaggtcatg gcctggatca tcctgttcat cgcgggtctg ttcgagatcg
     1621 gctgggccgt gggcctgaag ttcactgaag gcttcaccaa gcccattccc atcgccctga
     1681 cggcgatcag tctggtgctg tcgatgggtc tgctgggatg ggcggtgaag accctgccgc
     1741 tgggcaccgc ctatgcggtc tggaccggca tcggcgccgt tggcacggcc attgtcggca
     1801 ttgtggtctt caaggagccg gcg
//
LOCUS       DAOMEO010000707         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6646_length_1821_cov_2.615515, whole genome shotgun sequence.
ACCESSION   DAOMEO010000707 DAOMEO010000000
VERSION     DAOMEO010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_1821_cov_2.615515"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1821
                     /locus_tag="PLH31_14345"
     CDS             <1..>1821
                     /locus_tag="PLH31_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90411.1"
                     /translation="NLVLRRRFRATTLEAGARLPTQSGGGDTVDVRLNQLRIQRDNRF
                     MVDAKVSDQSELYETERDLAQPRSDAAFRSLIPRQTTWELNSVFSRPIKDGVSGTVNA
                     SLEGSDSTSWLGLNTLATSPLRRQSESLTGHVGGGMIGAIKGWKWSVTGNAERGKNDS
                     TTERVISGVAYRDISNAISTSADVQGDLSGTLFRIPAGAVSSTITGGFETVRLESDSV
                     RAGAARSGELSRDTGSAKLNLDLPLTSVRNDVLPGLGNLSLNLNLAADRLSDFGTLTT
                     LGYGANWSPIEKLRVIASVTRDQGAPSLQQIGDPEQLTPGVQVYDFRRGETAVISRLT
                     GGNRALREEEGRVIKLGLSLKPFDKTNLTLTTNYTNSRTDDLISSFPTATSQIEEAFP
                     ERFVRDATGRLVQIDARPINFDRRQTDELRTGFTFRKAFGPQPQPGRFRQRQAQAQAG
                     APAAGQPGPTPGPASATSEPPPPPGVDGAPPATTPTRPQGGERGPSGFGPGGFGGGGR
                     GFGGGGPPGAGTFQIGLYHTVKFTDEIVIRPGLPTLDLLDGAALGSGGGTPKNQIDLQ
                     GNVSRNGLGAGFNARWQEDTVVRGSGGVGAQDLSFSSLTKV"
BASE COUNT          293 a          670 c          609 g          249 t
ORIGIN      
        1 aacctggtcc tgcgtcgccg cttccgcgcc accaccctgg aggccggcgc ccggctgcct
       61 acccagtcgg gcggcggcga tacggtcgac gtccgcctca accagctgcg catccagcgc
      121 gacaaccgct tcatggtcga cgccaaggtc agcgaccaga gcgagctcta cgagaccgag
      181 cgcgatctgg cccagccacg ctcggacgcg gccttccgca gcctgatccc cagacagacc
      241 acctgggagc tcaacagcgt gttctcacgt ccgatcaagg acggggtctc gggtacggtc
      301 aacgccagcc tcgagggcag cgacagcacc agctggctgg gcctcaacac cctggcgacc
      361 agccccttgc gtcgccagtc ggaaagcctg accggccatg tcggcggcgg gatgatcggc
      421 gcgatcaagg gctggaaatg gtcggtgacc ggcaacgccg agcgcggcaa gaacgacagc
      481 accaccgagc gggtgatcag cggcgtcgcc tatcgcgaca tctccaacgc gatctcgacc
      541 tcggccgacg tgcagggcga tctcagcggc acgctgtttc gcatcccggc cggcgccgtt
      601 tcctcgacga tcaccggcgg ctttgagacc gtgcgcctgg aaagcgactc ggtgcgggcc
      661 ggcgcggcgc ggtcgggcga gctgtcgcgc gacaccggca gcgccaagct gaacctcgac
      721 ctgcccctga ccagcgtgcg caacgacgtg ctgccgggac tgggcaatct gtcgctcaat
      781 ctgaacctcg ccgccgacag gctttcggat ttcgggaccc tcaccaccct cggctacggc
      841 gccaactggt cgccgatcga gaagctgcgg gtcatcgcct cggtcacccg cgaccagggc
      901 gcgcccagcc tgcagcagat cggcgatccc gagcagctga cgcctggcgt gcaggtctat
      961 gacttccgcc gcggcgagac ggcggtgatc tcgcggctga ccggcgggaa ccgggcgctg
     1021 cgcgaggaag agggccgggt gatcaagctg ggcctcagcc tcaagccctt cgacaagacc
     1081 aacctgaccc tgacgaccaa ctacaccaat tcccgcaccg atgacctgat ctccagcttc
     1141 ccgaccgcga ccagccagat cgaggaggcc ttccccgagc gtttcgtacg cgacgcgacc
     1201 gggcgcctgg tgcagatcga cgcgcggccg atcaatttcg accgccgcca gaccgacgag
     1261 cttcgcaccg gtttcacctt ccggaaggcg ttcggtccgc agccgcagcc cggccggttt
     1321 cggcaaaggc aggcccaggc gcaagccggc gcgcccgcag cgggtcagcc gggcccgacg
     1381 cccggcccgg ccagcgcgac ctccgagccg cccccgcccc ccggtgtcga cggcgcgccg
     1441 cctgcgacca ccccgacgcg gcctcagggc ggcgagcgcg gccccagcgg ctttggaccg
     1501 ggcggttttg gcggcggcgg gcgtggcttc ggcggcggcg gaccgccggg cgcgggcacg
     1561 ttccagatcg ggctctatca cacggtcaag ttcaccgacg agatcgtcat ccggcccggt
     1621 ctgccgaccc tggacctgct ggacggcgcc gcgctgggct ccggcggcgg tacgcccaag
     1681 aaccagatcg acctgcaggg caatgtgtcc cgcaatggcc tgggcgccgg tttcaatgcg
     1741 cgctggcagg aggacacggt ggttcgcggt tcgggcggcg taggcgcgca ggacctgtcg
     1801 ttctcgtccc tgaccaaggt c
//
LOCUS       DAOMEO010000708         1819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6657_length_1819_cov_2.678005, whole genome shotgun sequence.
ACCESSION   DAOMEO010000708 DAOMEO010000000
VERSION     DAOMEO010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_1819_cov_2.678005"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="ispH"
                     /locus_tag="PLH31_14350"
                     /gene_synonym="lytB"
     CDS             <1..932
                     /gene="ispH"
                     /locus_tag="PLH31_14350"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQR90412.1"
                     /translation="LTIVLASPRGFCAGVDRAIQIVERAIEKFGAPVYVRHEIVHNRH
                     VVDRLKALGAVFIEELSEAPDDRPIVFSAHGVPKSVPAEAKAREMIYLDATCPLVSKV
                     HVEAQKHFNSGREIVLIGHAGHPEVVGTMGQLPEGTVTLIENIDDARAWTPKDASNVA
                     FLTQTTLSVDDTADMVDLLKARFPGIAAPHKEDICYATTNRQDAVKHLAQGTELVLVV
                     GSRNSSNSVRLAEVGLKAGARDARLIDDAHGLDWSWFEGVSRVGLTAGASAPEDLVQG
                     VIDAISAHFEVTIEDLIEARETVTFKLPRLLTT"
     gene            976..>1819
                     /gene="thrB"
                     /locus_tag="PLH31_14355"
     CDS             976..>1819
                     /gene="thrB"
                     /locus_tag="PLH31_14355"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQR90413.1"
                     /translation="MAVYTDITDEELSAFLADFDLGQALAFKGIAEGVENSNFLLETE
                     AGRFILTVYEKRAAPEDLPYFLNLMRWLADKGFPSATPITDRAGRTLKTLRGKPAALV
                     QFLPGLSVRRPTVAHCREAGEGLAQLHLAGEGYPGRRVNDLGQPYWAPLFSKHRKGAD
                     DLKPGLAATIEKDLAELALAWPRDLPSGVIHADYFPDNVFFRPDGKFAAAIDFYFACD
                     DAYAYDVAVTLNAWCFEQDGSFNATAAAALLNGYQRRRPLSPAEKQALPILARGAAMR
                     FFLTR"
BASE COUNT          304 a          638 c          567 g          310 t
ORIGIN      
        1 cgctcaccat cgtgctcgcc agcccccggg gcttctgcgc gggggtcgat cgcgccatcc
       61 agatcgtcga gcgcgccatc gagaaattcg gcgcgccggt ctatgtgcgt cacgagatcg
      121 tccacaatcg ccacgtcgtc gatcgcctga aggcgctggg cgcggtgttc atcgaggagc
      181 tgtcggaagc cccggatgac cgcccgatcg tgttctcggc ccacggcgtg cccaagtcgg
      241 tgccggccga ggccaaggcg cgcgagatga tctatctgga cgccacctgc ccgctggtgt
      301 ccaaggtcca tgtcgaggcc cagaagcact tcaattcagg acgcgagatc gtactgatcg
      361 gccacgccgg ccacccggaa gtggtcggca ccatgggcca actgcccgaa ggcacggtga
      421 ccctgatcga gaacatcgac gacgcccgcg cctggacccc gaaggacgcc tctaacgtgg
      481 ccttcctgac ccagaccacc ctgtctgtcg atgatacggc cgatatggtc gaccttctca
      541 aggcccgctt ccccggcatc gccgcgccgc acaaggaaga catctgctac gccaccacca
      601 atcgccagga cgcggtcaag catctggccc agggcacaga gctggtgctg gtggtcggtt
      661 cgcggaactc ttccaactcg gtgcgtctgg cggaggtcgg tctgaaggct ggcgcgcggg
      721 acgctcgcct gatcgacgac gcccatggcc tggactggtc ttggttcgag ggcgtttctc
      781 gcgtgggcct gaccgccggc gcctcggccc ccgaggatct ggtgcaaggc gtcatcgacg
      841 ccatctccgc gcattttgag gtgaccatcg aagacctgat cgaagcccgc gaaacggtga
      901 ccttcaagct gccgagactg ctcaccacct aggcggcctc ttgaccgccg gcgccggctc
      961 ccgcatcccc cgatcatggc cgtctatacc gacatcaccg atgaagaact gtccgccttc
     1021 ctggccgact ttgacctggg gcaggccctg gcgttcaagg gcatcgccga aggcgtggag
     1081 aactccaact tcctgctgga gacggaggcc ggtcggttca tcctgacggt ctatgaaaag
     1141 cgcgcggcgc cggaagacct gccctatttc ctgaacctga tgcgctggct ggcggacaag
     1201 ggtttcccaa gcgccacgcc gatcaccgat cgcgccggtc gcacgctgaa gaccctgcgc
     1261 ggcaagccag cagccctggt gcagttcctg cccggcctat cggtgcgtcg ccccaccgtc
     1321 gcccattgcc gcgaagccgg cgaaggtctg gcccagttgc atctggcggg cgaaggctat
     1381 ccggggcgcc gggtcaatga tctgggccag ccttactggg cgccgctgtt ttcaaagcac
     1441 cgcaagggcg ccgacgacct gaagcccggt ctggcggcga ccattgaaaa ggacctggcc
     1501 gagctggccc tggcctggcc ccgcgacctg ccgtcgggcg tgatccacgc agactatttc
     1561 cccgacaacg tcttcttccg gcccgatggc aagttcgccg ccgccatcga cttctatttc
     1621 gcctgcgacg acgcctatgc ctatgacgtt gcggtgacgc tgaacgcctg gtgcttcgag
     1681 caggacggaa gcttcaacgc caccgccgca gccgccctgc tgaacggcta tcagcgccgt
     1741 aggccgctca gccccgccga gaagcaggcc ctgccgatcc tggcgcgcgg cgcggcgatg
     1801 cggttcttcc tgacccgtc
//
LOCUS       DAOMEO010000709         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6662_length_1817_cov_2.696368, whole genome shotgun sequence.
ACCESSION   DAOMEO010000709 DAOMEO010000000
VERSION     DAOMEO010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_1817_cov_2.696368"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>302)
                     /locus_tag="PLH31_14360"
     CDS             complement(<1..>302)
                     /locus_tag="PLH31_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQR90414.1"
                     /translation="DGWLRTLDGQGLPSLTVPAPDLPPHPFPATPVRADFDAPSLPID
                     FQWLRSPWPDELFSLTARPGWLRLYGRESMGSQYRQALVARRQQAFCYSAATLMDF"
     assembly_gap    303..312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(313..1255)
                     /locus_tag="PLH31_14365"
                     /pseudo
     CDS             complement(313..1255)
                     /locus_tag="PLH31_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516413.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            complement(1192..>1817)
                     /locus_tag="PLH31_14370"
     CDS             complement(1192..>1817)
                     /locus_tag="PLH31_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90415.1"
                     /translation="FKVLKQRNFWGIALPRFLADPTWGTLAFWVPLYLTSVRGFDLKQ
                     IAMFAWLPFVAADLGCMFGPTVATFLQKRGVSLINARRWAFTVGALMMTGMAFVGRVE
                     SPYAAIALLCLGGFAHQTLSVTVITLSSDLFKRNEVATVAGMAGTMGNLGVLIFSLLI
                     GGLVATVGYDPFFIALGVLDLLGAVLLWTLVRDRPAPAPHAASPVSV"
BASE COUNT          320 a          566 c          662 g          259 t
ORIGIN      
        1 tcgaaatcca tcagggtcgc ggcgctgtag cagaaagcct gctgccgacg ggcgaccagc
       61 gcctggcgat attggctgcc catgctctcg cggccgtaaa gtcgcagcca gccggggcgg
      121 gcggtcaggc tgaacagctc gtccggccag ggcgagcgca gccactggaa gtcgatgggc
      181 aggctcggcg cgtcgaagtc ggcccgaacc ggcgtcgccg ggaagggatg tggcggcagg
      241 tcgggggcag gaaccgtcag gctgggcagg ccctggccgt caagcgtgcg cagccagccg
      301 tcnnnnnnnn nnggatcgcc gtctcgcggc ccatcacgca gcggccccgg ttgggcagtg
      361 ggcggccgca gagatagacg gcccaggtct gaccgtccgg agtctcgacc aggtcggcat
      421 ggccggcgcg ttgcagcggg gcgtcggggc gggtgcgcga gctgagaata tgggtgtcgg
      481 ggtgcagctc gtacgggcct gcgagatctc gcgaccgcgc catggtcacc gcatggttcc
      541 agcccgtgcc gccctcggcg gtgatcagat agtaccagcc atcgcgcttg tagatgtgcg
      601 gcgcctcggt caggcccagc ggcgtgccct cgaagatcac ctgccgctcg ccggtcaggg
      661 cgcgcgcgtt gtgggaatat tcctgcatca cgatcccggc gaagcggttg cggccggggc
      721 ggtggtccca cagcatgttg accagccatt tgcgaccgtc gtcgtcgtgg aacagcgagg
      781 ggtcaaagcc gctgctgttc agaaacaccg ggtccgacca ctcgccgtcg atggtgtcgc
      841 aggtgaccag atagttgtgg aagtcgcgca gggacgcgcc cgacgccccg cctacggtgg
      901 tgcggccata gcgcttcacg tcggtataga tcaggtggaa caggccatcg ctatgggtca
      961 ggcacggcgc ccacacgccg caggagtccg gcgcgcccag catgttcagc tggctgggcc
     1021 ggttcagcgg ccggctgatc agtcgccagt tcaccaggtc ccgcgagtgg tggatttgca
     1081 cgccggggaa ccactcgaag gtcgaggtgg cgatgtaata gtcctcgccc acccgcacga
     1141 tggacgtgtc gggattgaag cccggcagga tcggattgcg gatcatgagc tctagaccga
     1201 gacaggcgag gcggcgtgcg gcgccggggc cgggcggtcg cgaaccaggg tccacagcag
     1261 cacggcgccc aggaggtcga gcacgcccag ggcgatgaag aacggatcat agccgaccgt
     1321 ggcgaccagt ccgccgatca gcagcgagaa gatcagcacg cccagattgc ccatggtgcc
     1381 ggccatgccg gcgaccgtcg ccacctcgtt gcgcttgaac aggtcggacg acagggtgat
     1441 caccgtcacc gacagggtct ggtgcgcgaa gccgcccagg cacagcaggg cgatggcggc
     1501 ataggggctc tcgacccggc cgacgaatgc catgccggtc atcatcaggg cgccgaccgt
     1561 gaaggcccag cggcgggcgt tgatcaggct gacgccgcgc ttctgcagga aggtcgctac
     1621 cgtggggccg aacatgcagc ccaggtcggc ggccacgaac ggcagccagg cgaacatcgc
     1681 gatctgcttg aggtcgaagc cccgaacgct ggtcaggtac agcggcaccc agaaggccag
     1741 ggtgccccag gtgggatcgg cgagaaaacg gggcagggca atgccccaga agttgcgctg
     1801 tttcagaacc ttgaaga
//
LOCUS       DAOMEO010000710         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6676_length_1814_cov_3.023877, whole genome shotgun sequence.
ACCESSION   DAOMEO010000710 DAOMEO010000000
VERSION     DAOMEO010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_1814_cov_3.023877"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLH31_14375"
     CDS             <1..659
                     /locus_tag="PLH31_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR90416.1"
                     /translation="IGVIDEIAFQTNLLALNAGVEAARAGEAGRGFAVVAQEVRALAQ
                     RSADAAKEIKTLISTSTQQVGQGVSMVGQTGEALQAIVAKVSEIDALVSEIAAGGAEQ
                     ATGLNEVNAAVNQMDQTVQQNAAMVEQSTAASHSLKGEASNLMQMISRFQVSGSSGST
                     ARAPAARRPSAPAPATRPTLQPASSSSRPGANPVRAAQAKLAAFAGSAKPAADEWEEF
                     "
     gene            940..1314
                     /locus_tag="PLH31_14380"
     CDS             940..1314
                     /locus_tag="PLH31_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQR90417.1"
                     /translation="MIESLPRREREVFETLCRMEASTAAGVRAALSDPLSDSAVRTML
                     SRLEAKGLVERSSGPDGFLFSPVQRTEAVAASALRRMIDTFFAGSAASAATALLGLGQ
                     RLTPEEAAALERVIDKAVEKTS"
     gene            1311..>1814
                     /locus_tag="PLH31_14385"
     CDS             1311..>1814
                     /locus_tag="PLH31_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HQR90418.1"
                     /translation="MTWALLLSLLVKSSLVAGAGLACARFLAQRPVDRVDILRGAVCL
                     LLALPVIMNVLPTLDLALLPPPSSAPVSGPVPASTGQAAAIALTWPPSVQVLGGLWLL
                     GVVAVAGRLQLGLLTLHRWTREGRPVTCPAWTQPLEQLSPADQPRLVTSDRIASPLSW
                     GVTPGFII"
BASE COUNT          261 a          690 c          572 g          291 t
ORIGIN      
        1 tcatcggcgt catcgacgag atcgccttcc agaccaacct gctggccctc aacgccggtg
       61 ttgaagcggc tcgcgccggt gaagccggtc gtggcttcgc cgtcgtcgcc caggaagtgc
      121 gggccctggc ccagcgctcc gccgacgccg ccaaggaaat caagaccctg atctcgacct
      181 cgacccagca agtgggtcag ggcgtgtcga tggtgggcca gacgggcgag gccctgcagg
      241 ccatcgtcgc caaggtcagc gagatcgacg ccctggtcag cgagatcgcc gccggcggcg
      301 ccgagcaggc caccggcctc aacgaggtca acgccgccgt caaccagatg gaccagacgg
      361 tccagcagaa cgcggccatg gtcgagcagt ccaccgccgc cagccactcg ctgaagggcg
      421 aggccagcaa tctgatgcag atgatctcgc gcttccaggt cagcggctcc agcggttcga
      481 cggctcgcgc cccggccgcc cgccgcccga gcgcgccggc cccggcgacg cgccccaccc
      541 ttcagccggc ctcgtccagc agccgtccgg gcgccaaccc ggttcgcgcc gcccaggcca
      601 agctcgccgc cttcgccggt tcggccaagc cagccgccga cgagtgggaa gagttctaaa
      661 tcctcgtctg acgcatttcc ttcacgcgaa acgcgtaagg gcttgcctca gagacgctct
      721 gttttcgtaa gcgtagcgta acgcgcccca caccctcttg gggcgttcct cccctgaaga
      781 ctactgaggc cccgccggga aactggcggg gcttttttct gtcgtgagcc ggagttcggc
      841 aggttaccgc gatttcatct cgcgagcggt cttgatggct ggcgtcacta cacccgtagc
      901 gctacacatg tagcgaacct gtgtgtggtg gagcccgcca tgatcgaatc cctgccccga
      961 cgcgaacgcg aggtcttcga gaccctgtgc cggatggagg ccagcacggc cgccggcgtt
     1021 cgcgccgcgc tgtccgatcc tctcagcgac tcggccgtcc gcaccatgct ctcgcgcctg
     1081 gaggccaagg ggctggtcga gcgatcgagc ggtcccgacg gcttcctgtt cagcccggtc
     1141 cagcggaccg aggccgtcgc cgccagcgcc cttcgccgca tgatcgacac cttcttcgcc
     1201 ggctcggccg ccagcgccgc caccgccctg ctgggcctgg gccagcgcct gacccccgag
     1261 gaagccgccg ccctcgaaag ggtcatcgac aaggccgtgg agaagacgtc gtgacctggg
     1321 ccctgctcct gtccctgctg gtcaagtcca gcctggtcgc cggcgccggt ctggcctgcg
     1381 cccgcttcct ggcccagcgc cctgtcgatc gggtggatat cctacgcggc gcggtctgcc
     1441 tgctgctggc cctgcccgtg atcatgaacg ttctgcccac cctggatctg gcgctgctgc
     1501 cgccgccttc atccgcgccg gtgtcaggac ccgttcccgc ctctacaggc caggccgccg
     1561 caatcgccct gacctggccc ccgtccgtcc aggtgctcgg cggcctctgg ctgctgggcg
     1621 tggtcgccgt cgcgggccga ctgcagctgg ggctgctcac ccttcaccgc tggacccgcg
     1681 agggccgccc agtgacctgc ccggcctgga cacagccgct tgagcaacta tcgcccgccg
     1741 accagcctcg cctggtgacc agtgacagga tcgccagccc gctcagctgg ggcgtcacgc
     1801 ccggcttcat catc
//
LOCUS       DAOMEO010000711         1814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6681_length_1814_cov_2.217169, whole genome shotgun sequence.
ACCESSION   DAOMEO010000711 DAOMEO010000000
VERSION     DAOMEO010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_1814_cov_2.217169"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLH31_14390"
     CDS             complement(<1..676)
                     /locus_tag="PLH31_14390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQR90419.1"
                     /translation="MSFRATDRKIWDTTATLEALGAADVALWVWEPEADRLRVNGAAR
                     ALGLGPLAPECSSAAFRALALPQDRAQAEEVLKFREPGSEVIARLRMRGFETCIWRGV
                     WLEEGVRAAGVVAPEVKFAASGRCDLTGLLDRKSFIARARERLRHDGVHELVVADVDR
                     LRRLNEALGHERADLVLAALGSRLAAAFPAGSILGRIGEDEFAVLCEPKGFEPAELLR
                     NALEQPL"
     gene            complement(722..1282)
                     /locus_tag="PLH31_14395"
     CDS             complement(722..1282)
                     /locus_tag="PLH31_14395"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQR90420.1"
                     /translation="MFRSRPGLKLQGNGVYLRPPRVSDHRAWADLRRGSHTFLQPWEP
                     TWPEDDLGRRSYRARLGAYARELETGEAWPFFVFRKSDDVLIGAVRLFHVRRGVSLSG
                     TVGYWVGQPYTRQGHMTDAVATVIRFAFEGLGLHRLEAACMPENTASAALLLKCGFSG
                     EGYAPAYLKINGEWRDHRLFGLVARS"
     gene            complement(1327..>1814)
                     /locus_tag="PLH31_14400"
     CDS             complement(1327..>1814)
                     /locus_tag="PLH31_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQR90421.1"
                     /translation="LGGGMSSRLFQEAREKRGLAYNIDAYSDTYADHGALGIYAGCAA
                     GNAVETARVCAEQLVALARHIEPSELARAKAQLKAHMFMAREAALSRAEQSAGQVLLF
                     DRLYPPAELAAEVDAVTPADVTRLGERLLGSGRCATAILGAKSALKAGPAFETALFAA
                     A"
BASE COUNT          303 a          626 c          643 g          242 t
ORIGIN      
        1 gcaggggctg ttccagggcg ttgcgcagca gctcggccgg ctcgaagccc ttgggctcgc
       61 acagcacggc gaactcatcc tcgccgatgc ggcccaggat ggacccggcc gggaaggcgg
      121 cggccaggcg cgagcccagg gcggccagca ccaggtcggc gcgctcgtgg ccaagggcct
      181 cgttcaggcg gcgcaggcga tcgacatcgg cgaccaccag ctcgtggacg ccatcgtggc
      241 gcagccgctc gcgggcccgg gcgatgaagc tcttgcgatc cagcaggccg gtcaggtcgc
      301 agcggccgga agccgcgaat ttcacttcgg gggcgacgac gccggcggcg cggacgccct
      361 cctccagcca gacgccccgc cagatgcagg tctcgaagcc gcgcatgcgc agccgggcga
      421 tgacttccga gccgggctcg cggaatttca ggacttcctc ggcctgggcc cggtcctggg
      481 gcagggccag ggcgcggaag gcggcggagg agcattcggg ggccagcggg ccgaggccca
      541 gggcgcgggc ggcgccattg acgcgcaggc ggtcggcctc aggctcccag acccacaggg
      601 cgacatcggc cgcgcccaac gcttccagcg tggcggtcgt gtcccagatc ttgcggtccg
      661 tagcccgaaa cgacattctc gatcccacgc ctttttggcg tctctcgccg tgaccgccgc
      721 gctaggatcg cgcgacaagt ccgaacagac gatgatctcg ccattcgccg ttaattttca
      781 aataagcggg agcataaccc tccccggaaa agccgcattt cagcaaaaga gcggccgagg
      841 cggtgttttc cggcatgcag gcggcttcca ggcgatggag ccccaacccc tcgaaagcga
      901 agcggatgac ggtggcgaca gcgtcggtca tgtggccctg ccgcgtatag ggctggccga
      961 cccagtagcc gaccgtgccg ctcagcgaga cgccccgccg aacgtggaac agccgcaccg
     1021 cgccgatcag cacgtcatcg ctcttgcgga acacgaagaa cggccaggcc tcgccagtct
     1081 caagctcgcg ggcataggcc cccagccggg cgcgatagga ccggcggccc agatcatcct
     1141 ccggccaggt gggctcccag ggctgcagga acgtgtgcga gccgcgccgc agatcggccc
     1201 aggcgcgatg atccgagacc cgcggcggcc gcagatagac gccgttgccc tggagcttca
     1261 ggccgggtct ggagcggaac atctgcagaa gggtcgacac gaaggtcctc ggcggaagga
     1321 cggccctcag gccgccgcaa acagggcggt ctcgaaggcc gggcccgctt tcagggccga
     1381 cttggcgccg agaatggcgg tggcgcaacg ccctgaaccc agcagtcgtt cacccaggcg
     1441 cgtcacatcg gccggcgtga cggcgtcgac ctcggcggcc agctcggcgg gcggatacag
     1501 gcggtcaaac agcagcacct gaccggctga ctgctcggcg cgcgacaacg ccgcctcgcg
     1561 ggccatgaac atgtgggcct tcagctgggc cttggcgcgg gccagttcgc tgggctcgat
     1621 gtggcgggcc agggcgacca gctgctcggc gcagaccctg gccgtttcga ccgcgttgcc
     1681 cgctgcgcag ccggcataga tgcccagcgc cccgtgatcg gcataggtgt cggaataggc
     1741 gtcgatattg taggcgaggc cgcgcttctc acgggcttcc tggaacaggc gcgatgacat
     1801 gccgccgccc aggc
//
LOCUS       DAOMEO010000712         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6693_length_1811_cov_2.757973, whole genome shotgun sequence.
ACCESSION   DAOMEO010000712 DAOMEO010000000
VERSION     DAOMEO010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_1811_cov_2.757973"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLH31_14405"
     CDS             <1..390
                     /locus_tag="PLH31_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HQR90422.1"
                     /translation="GWPYSYFGQTVDKRVKPERPDLVAKALAPDYALGAHTASLGLTF
                     YEGASFPASYQGGIFIGQHGSWNRKPVSGYKVVFIPFANGKPAGPPQDFLTGFLDGEG
                     QAQGRPVGVVVDKVGALLVADDVGNVI"
     gene            476..>1811
                     /locus_tag="PLH31_14410"
     CDS             476..>1811
                     /locus_tag="PLH31_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HQR90423.1"
                     /translation="MKIERLPPVVTSLGHTNHPYMTGAWTPQHEEVNAWDLEVLEGAI
                     PTDIDGVYLRNTENPVHAPLGRYHPFDGDGMVHQIEFKGGQATYRNRFIRTRGFEAEQ
                     EVGESLWGGLADGPGTSKRPGFGAHGGLKDTASTDIVVHGGEAIATFYQCGEAYRLDP
                     LTLENLGVAAWAPLDGVSAHPKVDEETGELLFFNYSKHAPYMHYGVVDASGKRTTYQA
                     IDLPGPRLPHDMAFTKNYSILNDLPVFWDAALLERDIHAVRLHKGVPSRFGIVPRHGG
                     AVRWFEAAPTYVLHWLNAYEDGDEIVLDGYFQENPTPRPLEDAPDGHGHLMAYLDEHS
                     FRPKLHRWRFNLKTGETTEGHLDERILEFGMFNQQYAGRPYRYAYSTTAKPGWFLFNG
                     FVKHDLETGESWSIQLPEGRYASEAPFAPRIGATSEDDGYLVSFIIDENRGTS"
BASE COUNT          318 a          630 c          570 g          293 t
ORIGIN      
        1 ggctggccct acagctattt cggccagacg gtggacaagc gggtcaagcc cgagcgtccc
       61 gatctggtgg ccaaggccct cgcgcccgac tacgccctgg gggcccacac cgcctcgctg
      121 ggcctgacct tctatgaagg cgccagcttc ccggccagct accaaggcgg gatcttcatc
      181 ggccagcacg gctcgtggaa ccgcaagccg gtgtcgggct acaaggtggt gttcatcccg
      241 ttcgccaacg gcaagccagc gggtccgccg caggacttcc tgaccggctt cctcgacggc
      301 gaaggccagg cccagggccg ccccgtcggc gtcgtcgtcg acaaggtcgg cgccctgctg
      361 gtggccgacg atgtgggcaa tgtgatctga cgggtcgcgg ccgcgcgctg accaacctta
      421 cttgcacaac gagactgact ggcggtaagc tggcctatct ctgatggggt ctgccatgaa
      481 gatcgaacgc ctgccgcccg tcgtcacctc gctgggtcac accaatcacc cctatatgac
      541 cggggcctgg acgccgcagc atgaggaggt caacgcctgg gacctggagg tgctggaggg
      601 cgcgatcccg accgacatcg acggcgtcta tctgcgcaac accgaaaacc ccgtccacgc
      661 gccgctgggc cgctatcacc cgttcgacgg cgacggcatg gtgcaccaga tcgagttcaa
      721 gggcggccag gcgacctatc gcaaccgctt catccgcacg cgcggcttcg aggccgagca
      781 ggaagttggt gaaagcctgt ggggcggtct cgcggatggt ccgggaacct ccaagcgtcc
      841 gggctttggc gcccatggcg gcctgaagga caccgccagc accgacatcg tcgtccacgg
      901 cggcgaggcc atcgccacct tctaccagtg cggcgaggct taccgcctgg atcccctgac
      961 cctggaaaat ctcggcgtcg cggcgtgggc gccactggac ggcgtctcgg cccaccccaa
     1021 ggtcgacgaa gagaccggcg agctgctgtt cttcaactac tccaagcacg cgccctacat
     1081 gcactatggc gtggtcgatg cttcgggcaa gcgcaccacc tatcaggcca tcgacctgcc
     1141 ggggccgcgc ctgccgcacg acatggcctt caccaagaac tattcgatcc tcaacgacct
     1201 gccggtgttc tgggacgccg ccctgctgga gcgcgacatc cacgccgtgc gtctgcacaa
     1261 gggcgtgccc tcgcggttcg gcatcgtgcc gcgccatggc ggcgcggtgc gctggttcga
     1321 ggccgcgccg acctatgtgc tgcattggct aaatgcgtac gaggacggcg acgagatcgt
     1381 gctcgacggc tatttccagg aaaatcccac gccgcgtccg ctggaggacg cccccgacgg
     1441 tcacggccac ctgatggcct atctggacga gcacagcttc cggcccaagc tgcaccgctg
     1501 gcgcttcaac ctgaagaccg gcgagaccac cgagggccat ctggacgagc gcattctcga
     1561 gttcgggatg ttcaaccagc aatatgccgg ccggccctat cgttatgcct attccaccac
     1621 ggccaagccc ggctggttcc tgttcaacgg cttcgtcaag cacgacctgg agaccggcga
     1681 gagctggtcg atccagctgc ccgaaggccg ctatgccagc gaagcgccgt tcgcgccgcg
     1741 catcggggcg acctccgaag acgacggcta tctggtcagc ttcatcatcg acgagaaccg
     1801 cggaacgtcc g
//
LOCUS       DAOMEO010000713         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6696_length_1811_cov_1.869021, whole genome shotgun sequence.
ACCESSION   DAOMEO010000713 DAOMEO010000000
VERSION     DAOMEO010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_1811_cov_1.869021"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLH31_14415"
     CDS             <1..678
                     /locus_tag="PLH31_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQR90424.1"
                     /translation="MGSRRLPGKAMATLQGEPMIIRQLERLRSARCPSKIIVATSTDA
                     SDDALAGFVVSRGYTVYRGAGSDILDRIARCAETISSVSHVVRIKGDAPFVDPGIVDE
                     AVRLALASGADYTSNHVQRTYPAGLEVEVVTANALRVSAAQARDPMARVSPTAAIRND
                     PARFSQAHLKARRDWSHLNWRVKTAADLAFARSIYDALHPVDPGFCMNDVLDLVESHQ
                     DLARFAA"
     gene            complement(810..1811)
                     /gene="ald"
                     /locus_tag="PLH31_14420"
                     /pseudo
     CDS             complement(810..1811)
                     /gene="ald"
                     /locus_tag="PLH31_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519062.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
BASE COUNT          268 a          638 c          623 g          282 t
ORIGIN      
        1 atggggtccc gccgtcttcc gggcaaggcc atggccacgc tccagggcga gccgatgatc
       61 atccgccagc tggagcgcct gcgcagcgcg cgctgtccca gcaagatcat cgtcgccacc
      121 agcaccgacg cttccgatga cgccctggcc ggcttcgtgg tctcgcgcgg ctatacggtt
      181 tatcgcggag ccgggtccga tattctcgac cgcatcgccc gctgcgccga aaccatcagt
      241 tcggtcagcc acgtcgtgcg catcaagggc gacgcgccct ttgtcgatcc cggtatcgtg
      301 gacgaggcgg tccgcctggc cctggccagc ggcgccgact acacatccaa ccacgttcag
      361 cgcacctatc cggccgggct tgaggtcgag gtggtcacag ccaacgccct gcgggtctcg
      421 gccgcacagg cgcgtgatcc gatggcccgc gtgtcgccca ccgccgccat tcgcaacgat
      481 ccggcccggt tctcgcaggc tcacctgaag gcccgccgcg actggtcgca cctgaactgg
      541 cgggtcaaga ccgccgccga cctggccttc gcgcggtcga tctacgacgc cctgcatccg
      601 gtcgatcccg gcttctgcat gaacgacgtg ctggacctgg tcgaaagcca tcaggacctg
      661 gcgcggttcg cggcctgacc ttaaaaactg tcatcccggc ccagcgcgta gcgcgccgag
      721 ccgggaccgc cgggaactcg gcgcgagacg tccggccgac cccagtgctt gcggcggccc
      781 cgggtcttcg cccgggatga cagcgtctgt tacgtccagg cgccgtaagg atcgaccgac
      841 ggcttgccca gcgcctgcgc gacggccggg tgggtcagct cgcccttcag cacattgaga
      901 ccgcgggcca ggtggacgtt ggtcttcagg gcgtccaggc cgtggtcggc cagggccagg
      961 ccgaacggca gggtcgcgtt gcccagggct tccgacgagg tgcgcggcgc ggcgccgggc
     1021 atgttggcga cgcagtagtg gacgacgccg tcgaccgtat aggtcggctc ggcgtgggtg
     1081 gtgggcttgc tggtctcgaa acagccgccc tggtcgatcg acacgtcgac cagcaccgag
     1141 cccggcttca tctgcttgag gtgctcgcga cgcaccagct tgggcgcggc ggcgccggcg
     1201 gtcagcacgg cgccgatgat cacgtcagct ttcaggatct cttcttccac ggccgcgaag
     1261 gtcgaatagc gggtcaggat gcggccctga tagaggtcgt ccagttcgcg catgcgcggg
     1321 atagagcgtt ccagcaccac gacttccgcg ccgaggccag cggccatgcg cgcggcgttg
     1381 gagccgacca cgccgccgcc cagcacggcc acgcgggccg ggggcacgcc cggcacgccg
     1441 cagagcagca ggcccatgcc gccattgtgc ttcagcaggg tctcgccggc cgagaacttc
     1501 cgacatcggc gccagcagcg gcaggccgcc gcgcgcgtcg gtgacggtct cataggcgat
     1561 ggccgcgcag ccgctgctga gcaggccctc ggtctgggcc ggatcgggcg ccaggtgcag
     1621 ataggtgaag aggatgtggc ggggttccag cttctcccac tcgaccttct gcggctcctt
     1681 gaccttgatg atcatgtccg cgccggagaa cacggctgcg gcgtcggcgg cgagggtcgc
     1741 gccagctttc agatagtcac cgtcggcata gcccgcgccg aggccggcgc cggactggac
     1801 cagcacgctg t
//
LOCUS       DAOMEO010000714         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6726_length_1804_cov_2.345340, whole genome shotgun sequence.
ACCESSION   DAOMEO010000714 DAOMEO010000000
VERSION     DAOMEO010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_1804_cov_2.345340"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="flgA"
                     /locus_tag="PLH31_14425"
     CDS             complement(<1..733)
                     /gene="flgA"
                     /locus_tag="PLH31_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HQR90425.1"
                     /translation="MKPLLITVCALALAGPALAGQAVSLKFDTTDADGRVTLGDLFDG
                     AGSASSVLVATRMGPTTVLDAGQVQLAARRAGLDWTNTQGVRRIIVRQGVDGGGNGAG
                     AVRARGNVEVLAYARSLSAGEIVQPQDLIWVKMAASPSDAPQDADALIGMSAKRPLRE
                     GAAASLRDVSAAQVIKNGDIITVIYENEGISLALQGKAMTAAAAGDVVSVQNTLSKKI
                     IQAVATGPGSAMVGPQAQSLQTRSPI"
     gene            complement(738..>945)
                     /locus_tag="PLH31_14430"
     CDS             complement(738..>945)
                     /locus_tag="PLH31_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HQR90426.1"
                     /translation="TGGSGPANIAAPGQPGFGTLMQNYTESSNVDAVSEITALIVAQR
                     AYEMNSKVITTADQMLQATSQLRS"
     assembly_gap    946..955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<956..>1396)
                     /locus_tag="PLH31_14435"
     CDS             complement(<956..>1396)
                     /locus_tag="PLH31_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HQR90427.1"
                     /translation="QTIERAGAQSSSDGNVVPTGVQIGGGVKAGSVYRITEQGTPTMT
                     NNELDVAIQGKGYLQILLPSGETAYTRAGNFSTNDQGQVVTDDGYTVQPGITVPQNAT
                     AITIGKTGLVQVTLQGQVEPQVIGQIELANFMNEGGLEAIGDNL"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1407..1503)
                     /locus_tag="PLH31_14440"
     CDS             complement(<1407..1503)
                     /locus_tag="PLH31_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HQR90428.1"
                     /translation="MQALRTAATGMSAQQLNVEVISNNIANMNTVG"
     gene            complement(1517..>1804)
                     /locus_tag="PLH31_14445"
     CDS             complement(1517..>1804)
                     /locus_tag="PLH31_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HQR90429.1"
                     /translation="QGPLALGKIGVVRPDDLSTLSKDGDNLYRNTANANLQPAPDAVV
                     HQGMLESSNVQSVVQITKLIEVQRAYESMAKMMDNTAELTRSAVERLGKAN"
BASE COUNT          256 a          534 c          649 g          345 t
ORIGIN      
        1 ggatgggaga acgggtttgc aaggactggg cctggggccc caccattgcc gagcccggcc
       61 ccgtcgcgac ggcctggatg attttcttgg acagggtgtt ctggacggag accacatcgc
      121 cggctgcggc ggcggtcatg gcttttcctt gcagggcaag ggaaatgccc tcgttttcgt
      181 agatcacggt gatgatgtcg ccgttcttga tcacctgcgc ggccgagaca tcgcgcagcg
      241 aggctgcggc gccttcacgc agggggcgct tggcgctcat gccgatcagg gcgtctgcgt
      301 cctggggggc gtcgctgggt gatgcggcca tctttaccca gatcaggtcc tggggttgca
      361 cgatctcgcc ggccgacaga ctgcgggcat aggcaagaac ttccacattg ccacgggcgc
      421 ggaccgcgcc agcgccattg ccgccgccgt cgacgccctg gcgcacgatg atccggcgca
      481 cgccctgcgt gttggtccag tccaggccgg cccgacgcgc ggccaactgc acctggcccg
      541 cgtccagaac ggtcgtgggg cccatgcggg tggcgaccag cacgctcgaa gccgagccgg
      601 cgccgtcgaa caggtcgccc agggtcacgc ggccgtcggc gtcggtggtg tcgaacttca
      661 gcgaaacggc ctggccggcg agggcggggc ccgccagagc cagggcgcag acggtgatca
      721 ggaggggctt catgagccta ggacctcagc tgcgaagtgg cctggagcat ctggtcggcc
      781 gtggtgatga ccttggagtt catctcgtag gcgcgctggg cgacgatcag ggcggtgatt
      841 tcgctgaccg cgtcgacgtt cgaggattcg gtgtagttct gcatcagggt gccgaaacca
      901 ggctggccgg gggcggcgat gttggccggg ccggagccgc cggttnnnnn nnnnnaacag
      961 gttgtcgccg atggcttcca gaccgccttc attcatgaag ttggccagtt cgatctggcc
     1021 gatgacctgg ggctcgacct ggccctgcag ggtcacctgg accaggccgg tcttgccgat
     1081 ggtgatggcg gtggcgttct gcgggacagt gatgccaggc tggacggtgt agccgtcgtc
     1141 ggtgacgacc tggccctgat cgttggtcga gaaattgccg gcgcgggtat aggcggtctc
     1201 gcccgagggc agcaggatct gcagatagcc cttgccttgg atggccacgt ccagctcgtt
     1261 gttggtcatg gtgggcgtgc cctgctcggt gatcctatag accgagccgg ccttcacgcc
     1321 gccgccgatc tggacgccgg tgggcaccac attgccatcc gaggacgact gggcgccggc
     1381 ccgttcgatg gtctggnnnn nnnnnnagcc aacggtgttc atgttggcga tgttgttcga
     1441 gatgacctcg acgttcagct gttgggccga catgccggtc gcggcggtgc gcagagcttg
     1501 catcgtctag tttcccttag ttggctttgc cgaggcgctc gacggcggag cgcgtcagtt
     1561 cggcggtgtt gtccatcatc ttggccatgc tctcgtaggc gcgctgcacc tcgatcagct
     1621 tggtgatctg gacgacggac tggacgttgg aggactccag catgccctgg tgcaccacgg
     1681 cgtcgggggc gggctggaga ttggcgttgg cggtgttgcg gtacaggttg tcgccgtcct
     1741 tgctcagcgt cgacagatcg tcggggcgaa ccacgccgat cttgcccaag gccagggggc
     1801 cctg
//
LOCUS       DAOMEO010000715         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6729_length_1803_cov_2.565789, whole genome shotgun sequence.
ACCESSION   DAOMEO010000715 DAOMEO010000000
VERSION     DAOMEO010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6729_length_1803_cov_2.565789"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(411..1142)
                     /locus_tag="PLH31_14450"
     CDS             complement(411..1142)
                     /locus_tag="PLH31_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HQR90430.1"
                     /translation="MLTTAEQMAWEFLSKAPHYAGPDEVETHFARAIAAFGYDRFCAT
                     LINADRYGREPPTLSRQDFETWDARYWSERYVVHDPCVKLLMKSTRSFAWSDARPFSD
                     NKRADIMWSEASDLGMNDGFVVRVFGPAGHLVLIRMATEALDTDPAARAVIESLATVF
                     GTIMLKHWELQDDNPDYGVISERQAQCLHWASRGKTDWEIGAILELSPRTVHHHIENA
                     KKRLGVAKRQEAVMKAGELGLLGKG"
     gene            1267..1752
                     /locus_tag="PLH31_14455"
     CDS             1267..1752
                     /locus_tag="PLH31_14455"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQR90431.1"
                     /translation="MPEACAGQRVPRISLGVLVGQEIQPRSFEQILAGRRAVVIGVPG
                     AFTPICTGLHVPDLISSADRLRASGIGLLICITPNDPWTMDAWARTVDPHGKLMFLSD
                     GNLALAKALGTTIRDTEHFLGLRTRRYLMQTRDAVIERLTVESSPLALTCTRAQDVII
                     D"
BASE COUNT          301 a          574 c          609 g          319 t
ORIGIN      
        1 gggaagggcg tccggccggc gaccagcgac ggctgtcagg ggtgatcgag cggggccacc
       61 ggagtcgcga gagcgggacc gggctaggct taaacgagga caggggacgt aaacctgtcc
      121 atcggggatc tgccagggtc cccgcccgtc cgaaggctcg ctccgggtcc tccggaatcg
      181 gcgtggcttg cagctttaag ggctgggtcc gtcgcgagga ggagaagccc gggtggggca
      241 aaaggttgaa caagacctgc gcgccccggc cagagggata acgatcacgc ggagcgtctg
      301 tcttcgtcga aacggtactc aactatccca tcatccgggc gaggcccgga cgctccgcga
      361 cccttcccac cagctcggcg gtttccgcgc gaggtcgaga cctcatgccc tcaacccttg
      421 cccaacaggc ccaactcccc cgccttcatc accgcctcct gccgcttggc cacgcccagg
      481 cgcttcttgg cgttctcgat gtggtggtgg accgtgcggg gggaaagttc caggatcgcg
      541 ccgatctccc agtcggtctt gccccggctg gcccaatgca ggcattgggc ctgacgctca
      601 gagatgacgc cgtagtcggg attgtcgtcc tgcagttccc agtgcttgag catgatcgtg
      661 ccaaacaccg tggccaggct ttcgatcacc gcccgcgcgg cggggtcggt gtccagggcc
      721 tcggtcgcca tgcggatcag caccaggtgg ccggcgggtc cgaagacgcg gaccacgaac
      781 ccgtcgttca tgcccaggtc cgaggcctcg gaccacatga tgtcggcgcg cttgttgtcg
      841 ctgaagggcc tggcgtccga ccaggcgaac gaccgcgtgg acttcatcag cagcttcacg
      901 cacgggtcgt ggaccacgta gcgttcgctc cagtaccggg cgtcccaggt ctcgaagtct
      961 tgccggctga gggtgggcgg ctccctgcca tagcggtcgg cattgatcag ggtggcgcag
     1021 aaccggtcat agccaaaggc cgcgatggcg cgggcgaaat gggtctcgac ctcgtcgggc
     1081 ccggcgtaat gcggcgcctt gctgagaaat tcccaggcca tctgctcggc ggtcgtgagc
     1141 acgtgacgtc tctccgttcg tggtccaagc tagaggctgg gatgcgccat gcccagcctc
     1201 aaacccactt caacggcatg gcggacaggt tatggcgcgc gatgagacag cgggcggagt
     1261 ttgccgatgc ctgaggcttg cgcgggccag cgtgttcccc ggattagcct gggtgtcctg
     1321 gtcggtcagg agatccagcc ccggtctttc gagcagatcc tggcgggacg gcgcgcggtg
     1381 gtgattggcg ttcccggcgc ctttacgccc atctgcacgg ggctgcatgt gcccgatctg
     1441 atcagcagcg ccgacaggct ccgggccagc ggcatcggcc tgctgatctg catcacgcca
     1501 aacgatccgt ggaccatgga cgcatgggcc agaacggtcg atccgcacgg caagctgatg
     1561 tttctctcgg atggaaacct ggccctggcc aaggccctgg gcacgacgat ccgtgacacc
     1621 gagcattttc tggggctgcg cacccggcgc tatctgatgc agacccggga cgccgtgatt
     1681 gagcggttga cggtggagag ctctcccctg gcgctgacgt gcacccgggc tcaggacgtc
     1741 atcatcgact agagcatttt ccgacgaagt ggacgccggt tcgtcgtcag aaaatgcgtt
     1801 aaa
//
LOCUS       DAOMEO010000716         1802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6738_length_1802_cov_1.918718, whole genome shotgun sequence.
ACCESSION   DAOMEO010000716 DAOMEO010000000
VERSION     DAOMEO010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_1802_cov_1.918718"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1802)
                     /locus_tag="PLH31_14460"
     CDS             complement(<1..>1802)
                     /locus_tag="PLH31_14460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQR90432.1"
                     /translation="AVHPSDERYKDLVGKFVTLPIVGRRIPIVADDYADPTKGSGAVK
                     ITPAHDFNDFGVGKRHGLDAINILTPDARLNDSVPADYQGLDRFVARKAIVARAEEDG
                     WLREIEKTKHMVPHGDRSGVVIEPYLTDQWYVDAKTLAQPALKAVENGDTVFEPRHWE
                     KTYFEWLRNIEPWCVSRQLWWGHRIPAWFGEDGHIFVEETEEEAYAAAILHYGDEAPV
                     LKRDEDVLDTWFSSALWPFSTLGWPEKTEDLARFYPTSTLVTGFDIIFFWVARMMMMG
                     LHFMGEVPFKQVFINALVRDEKGAKMSKSKGNVLDPLVLIDELGCDAVRFTMTAMSGQ
                     ARDIKLSKQRIEGYRNFGTKLWNASRFAQMNECIRVEGFDPATVQQPINKWIRGETVK
                     TVAEVTRALEAPSFDAAATALYKFIWNVFCDWYLELAKPVLNGDDAAAKAETRATAAW
                     ALDVILKVLHPVMPFITEELWDKTALFGPKRDGMLIVEKWPELPESWIDADAEAEIGW
                     LVDTVSEIRSVRAEMNVPPGAKPPLTLIGANAETRARLARHRDLLLTLARLDSAREAD
                     APPAGAVPFVLGEATGALAIAEFIDLAAEKARLT"
BASE COUNT          290 a          574 c          609 g          329 t
ORIGIN      
        1 ttggtcaggc gggccttttc ggccgccaga tcgatgaact cggcgatggc cagcgccccg
       61 gtcgcctcac ccagcacgaa gggcacggcg ccggccggcg gcgcgtcggc ctcgcgggcg
      121 ctgtcgaggc gggccagggt cagcagcagg tcgcggtgac gggccagtcg ggcccgggtc
      181 tcggcattgg cgccgatcag ggtcagcggc ggcttggcgc ccgggggcac gttcatctcg
      241 gcgcggaccg agcggatttc gctgacggta tcgaccagcc agccgatctc ggcctccgcg
      301 tcggcgtcga tccagctttc cggcagctcc ggccatttct cgacgatcag catgccgtca
      361 cgcttggggc caaacagggc ggtcttgtcc cacagctcct cggtgatgaa cggcatcacc
      421 gggtgcagaa ccttcaggat cacgtccagc gcccaggcgg ccgtggcgcg ggtctctgcc
      481 ttggcggcgg cgtcgtcgcc gttgaggacc ggcttggcca gttccagata ccagtcgcag
      541 aacacgttcc agatgaactt gtagagcgcg gtggcggccg catcgaacga cggcgcctcg
      601 agcgcgcggg tcacctcggc gacggtcttg accgtctcgc cgcgaatcca cttgttgatc
      661 ggctgctgga cggtcgcggg atcaaagccc tcgacgcgga tgcactcgtt catctgggca
      721 aagcgcgagg cgttccacag cttggtgccg aagttgcgat agccctcgat gcgctgcttc
      781 gacagtttga tgtcacgcgc ctggcccgac atcgccgtca tggtgaagcg caccgcgtcg
      841 cagcccagtt cgtcgatcaa gacgagcggg tccagcacat tgcccttgga cttgctcatc
      901 ttggcgccct tctcgtcacg gacgagggcg ttgatgaaca cctgcttgaa cgggacttcg
      961 cccatgaagt ggaggcccat catcatcatc cgggcgaccc agaagaagat gatgtcgaag
     1021 cccgtgacca gggtgctggt ggggtagaag cgcgccaggt cttcggtctt ttccggccag
     1081 cccagggtgg agaacggcca cagggccgag ctgaaccagg tgtcgaggac gtcttcgtcg
     1141 cgcttcagca ccggggcttc gtcgccatag tgcaggatgg cggcggcata ggcctcttcc
     1201 tcggtctcct cgacgaagat gtggccatct tcgccaaacc aagccgggat gcggtggccc
     1261 caccagagct ggcgcgagac gcaccagggc tcgatgttgc gcagccactc gaagtaggtc
     1321 ttttcccagt gacggggctc aaagaccgtg tcgccgttct cgaccgcttt cagggccggc
     1381 tgagccaggg tcttggcgtc gacgtaccac tggtcggtca gatagggctc gatgactacg
     1441 ccggaacggt cgccgtgcgg gaccatgtgt ttggtcttct cgatctcgcg cagccagccg
     1501 tcttcctccg cccgggcgac gatcgccttg cgggccacga aacgatccag gccctgatag
     1561 tcggccggca cgctgtcgtt caggcgcgca tcgggggtca ggatgttgat ggcgtccagg
     1621 ccgtgacgct tgccgacgcc aaagtcgttg aaatcatggg ccggggtgat cttgaccgcg
     1681 cccgagccct tggtcgggtc ggcatagtcg tcagcgacga tcgggatgcg acggccgacg
     1741 atcggtagcg tcacgaactt gccgaccaga tccttgtagc gctcatcgct gggatgaacg
     1801 gc
//
LOCUS       DAOMEO010000717         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6749_length_1798_cov_2.390706, whole genome shotgun sequence.
ACCESSION   DAOMEO010000717 DAOMEO010000000
VERSION     DAOMEO010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6749_length_1798_cov_2.390706"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PLH31_14465"
     CDS             <1..90
                     /locus_tag="PLH31_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HQR90433.1"
                     /translation="QRDGIHPNAAGARVIAERLAPTVAAALRA"
     gene            complement(114..>562)
                     /locus_tag="PLH31_14470"
     CDS             complement(114..>562)
                     /locus_tag="PLH31_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR90434.1"
                     /translation="LLVIARSSFSISTLIGILMLMGIAAKNSILLVEYAIMAMKEHGM
                     TKREAIIDAAHKRARPILMTTVAMGAGMAPVALGFGADVEFRAPMAIAVIGGLITSTL
                     LSLLYVPVVFVLMDGLRTRAERVTHRMFGRGAHTAHPGPAQSALVD"
     assembly_gap    563..572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<573..>1798)
                     /locus_tag="PLH31_14475"
     CDS             complement(<573..>1798)
                     /locus_tag="PLH31_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR90435.1"
                     /translation="VVARLLTPLMGAYMLKPNHKHEDRDPSWMAPYLKSLTWGLNNRW
                     KVLAMGIPMVLISGFLATKLPFEFQPAADRGRAPFSVELPPGATLDETDAIVQRMTKE
                     LLARPEVVGVYASIGGNNAVNTATLNADLVAKGERMGQQEFSRAMVDQFAAIPGARIG
                     AGSNNGGGPSDGTSFTLSLLSDNGPALEAAARKVEAEMRNVPGLAHVVNTAAIARPEI
                     VVSPRPDQAARAGVSAGVISQAVRVATIGDVDQSLPKYNLGDRQVPIRLRLTQDARED
                     MAVLQTLQVPTANGPVPLNAVADVRFGAGPSQISRQDRSRVATITAELNGIVVGEASK
                     RVHDLPSVKNLPAGVQEVAAGDVEFIQEMVTGFAAALITGILLMYVVLVLLFRSFAHP
                     ITIMVALPLAIGGAFA"
BASE COUNT          279 a          585 c          651 g          273 t
ORIGIN      
        1 cagcgcgacg gcatccatcc caacgccgcc ggcgcgcggg tgatcgccga gcgtctggcc
       61 ccgacggtgg cggcggcgct gcgggcctag gtcagaaccc acagcgccgg ccttcagtcg
      121 accagggccg attgagccgg gcccggatgc gccgtgtgcg caccccggcc gaacatccgg
      181 tgggtgacgc gctcggcgcg ggtgcgcagg ccgtccatca gcacgaacac caccggcaca
      241 tacagcagcg acagcagggt cgaggtgatc agaccgccga tgacggcgat ggccatcggg
      301 gcccggaact ccacatcggc gccaaagccc agggccacgg gcgccatgcc cgcgcccatg
      361 gccacggtgg tcatcaggat cggccgggcc cgcttgtggg ccgcgtcgat gatggcctcg
      421 cgcttggtca tgccgtgttc cttcatcgcc atgatcgcgt actcgaccag caggatcgag
      481 ttcttggccg cgatgcccat cagcatcagg atgccgatca gggtcgagat cgagaagctg
      541 gaccgcgcga tcaccagcag ggnnnnnnnn nnggcgaagg ccccgccgat cgccagcggc
      601 aaggcgacca tgatggtgat cgggtgggcg aagctgcgga acagcagcac cagcacgaca
      661 tacatcagca ggatgccggt gatcagggcg gcggcgaagc cggtgaccat ctcctggatg
      721 aactcgacgt cgccggccgc cacttcctgg acgccggctg gcaggttctt gactgagggc
      781 aggtcgtgga cgcgcttgct ggcctcgccc accacgatgc cgttcagttc ggcggtgatg
      841 gtggcgacgc gcgagcggtc ctgccggctg atctgcgagg gaccggcgcc aaagcgcaca
      901 tcggccacgg cgttcagcgg aacggggcca ttggcggtgg gcacctgcag cgtctgcaga
      961 acggccatgt cctcgcgggc gtcctgggtc aggcgcaggc ggatcggcac ctggcggtcg
     1021 cccagattgt atttcggcag ggactggtcg acatcgccga tggtggcgac ccgcacggct
     1081 tgcgagatca cgccagccga gacgccggcc cgggcggcct ggtcgggacg cggcgagacg
     1141 acgatttcgg gtcgggcgat ggcggccgtg ttgaccacgt gggccaggcc cggaacattc
     1201 cgcatctcgg cctcgacctt gcgggcggcg gcttccaggg cggggccgtt gtcgctgagc
     1261 aggctgaggg tgaagctggt gccgtcgctg ggaccgccgc cattgttgct gccggctccg
     1321 atccgcgcgc ccggaatggc ggcgaactgg tcgaccatgg cccggctgaa ctcctgctgg
     1381 cccatgcgct cgcccttggc gaccaggtcg gcgttcaggg tcgcggtatt gacggcgttg
     1441 ttgccgccga tcgaggcata gaccccgacc acttcgggac gggccagaag ttccttggtc
     1501 atgcgctgca cgatggcgtc ggtctcgtcc agcgtggccc cggggggcag ctccaccgag
     1561 aagggcgcgc ggccccggtc ggcggcgggc tggaattcga acggcagctt ggtggccagg
     1621 aagcccgaga tcagcaccat ggggatgccc atggccagca ccttccagcg attgttcagg
     1681 ccccaggtca ggctcttgag gtagggcgcc atccaggatg ggtccctgtc ctcatgcttg
     1741 tggttgggct tgagcatgta ggcgcccatc agtggcgtca gcaggcgggc cacgacca
//
LOCUS       DAOMEO010000718         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6755_length_1797_cov_1.936854, whole genome shotgun sequence.
ACCESSION   DAOMEO010000718 DAOMEO010000000
VERSION     DAOMEO010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6755_length_1797_cov_1.936854"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLH31_14480"
     CDS             <1..529
                     /locus_tag="PLH31_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR90436.1"
                     /translation="RDSDINMRGLAQELKVSPKLLYRYAASKDELLDLAAAALLETWI
                     APSDALPWSDRLTAVVGGARDLIERFPALSRAVLMRNLEARDSPEVSRVVASIKGCFA
                     DAGLSASEVDQVFLAYEMLILGELALSRAVRDGELSEARLPAAAARAVSIETSLRLMI
                     SGITVFHVGQTPCQT"
     assembly_gap    540..549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(559..747)
                     /locus_tag="PLH31_14485"
     CDS             complement(559..747)
                     /locus_tag="PLH31_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90437.1"
                     /translation="MASDNRPPDKHGARARLAEAADLRASAKLADRPQRSRALRRLAK
                     MTIAAAAALRPLTRRRPD"
     gene            complement(1005..>1797)
                     /locus_tag="PLH31_14490"
     CDS             complement(1005..>1797)
                     /locus_tag="PLH31_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR90438.1"
                     /translation="GDLAACRLGLMLDLETTGVDPRRDEIIEFALTPFHYDGEGRIVS
                     VDEAFSRLRQPAQPIPPEITRITGITDAMVAGQTVDPAEVSAFIAPAALIIAHNAQFD
                     RRFAEAFSPAFALKPWACSMSQIDWRAEGYEGTKLSYLATQGGFFHAGHRATNDCLAT
                     LEVLAAPLPIGGVTGLARLLQSARRASWRIWAENSPFDLKDVLKARGYRWNGDPGPAP
                     KAWYIDVDEADREAEVAFLRAEIYRGEIDPLMRRITAFERFSERA"
BASE COUNT          272 a          589 c          615 g          311 t
ORIGIN      
        1 tcgcgattct gatataaata tgaggggcct ggctcaggaa cttaaggttt ctcccaagct
       61 gctctatcgc tatgcggcca gcaaggacga gttgctcgac ctggcggcgg cggccctact
      121 cgagacctgg atcgccccgt cggacgcctt gccctggtcc gaccgactga ccgctgtagt
      181 cggcggcgcc cgtgacctga tcgagcgctt ccccgcgctg tcgcgggcgg tgctgatgcg
      241 aaaccttgag gcccgcgact ctccggaggt ctctcgtgtc gtggcttcca tcaagggctg
      301 tttcgccgac gccggactgt cggcgtccga agtcgatcag gtattcctgg cctatgagat
      361 gctgatcctg ggggagctgg ccttgtcccg ggcggtgcgt gacggcgagt tgtccgaagc
      421 gcgtctgcca gccgccgccg cccgcgctgt cagcatcgag accagtttgc ggctgatgat
      481 ctccggaatc accgtctttc acgttggcca aaccccctgc caaacctgat ctagaacggn
      541 nnnnnnnnnc ggcggcgtct agtccgggcg gcggcgggtc agcggtcgca gagcggcggc
      601 tgcggcgatg gtcatcttgg ccagccggcg cagggcccgg gaccgttgag gacgatccgc
      661 aagcttggcg gaggcccgca gatcggcggc ctcggccaga cgcgcgcgag ccccgtgttt
      721 gtcgggcggc cggttgtcgc tggccatcgg tgtctccaag gacgtcatta gtagaacgcc
      781 gttcttcgag tcggattcag gctccggccg atctctcgcg tctggagcgc gcgtcctgcg
      841 gcggctccac ttggcgcacc gcgcgccaat agggccaagg cttggcgcgc acctgctcga
      901 ccacgaacca ggcttcctcg ccctcgaagg gcgggacgtt gtcgacatgg atgaagctgt
      961 tgcgagcgcg cgaggccttt ggctggccgc ccgcgacgcc ggcctcaggc gcgctcggag
     1021 aaccgttcga acgccgtgat cctgcgcatg agcggatcga tctcgcccct atagatttcg
     1081 gctcgcagga aggcgacttc ggcctcgcga tcagcttcgt cgacatcgat gtaccaggcc
     1141 ttgggcgcgg ggccggggtc gccgttccag cgatagcccc gtgccttgag gacgtccttc
     1201 aagtcaaagg gcgagttttc cgcccagatc cgccaggacg cgcgccgggc gctctgtagt
     1261 aggcgcgcca ggcccgtcac gccgccgatc ggcagcggcg cggccagaac ctccagggtc
     1321 gccaggcagt cgttggtcgc gcgatgtccc gcgtgaaaga agccgccctg cgtggccaga
     1381 taggagagct tggttccctc gtagccctcg gcccgccaat caatctgcga catcgagcac
     1441 gcccagggct tgagcgcgaa ggccggcgag aaggcctcgg cgaaacgccg gtcgaactgg
     1501 gcgttgtggg cgatgatcag cgcggcgggc gcgatgaacg ccgacacctc cgccgggtcg
     1561 accgtctggc cggcgaccat ggcgtcggtg atgccggtga tgcgggtgat ttccggcggg
     1621 atcggctggg ccggctgacg caggcgcgag aaagcctcgt ccaccgagac gatgcggccc
     1681 tcgccgtcat agtgaaacgg cgtcagcgcg aactcgatga tttcgtcgcg acgcggatcg
     1741 acccctgtgg tctccagatc cagcatcagg cccagccggc aggcggccag atcgccc
//
LOCUS       DAOMEO010000719         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6758_length_1795_cov_2.697701, whole genome shotgun sequence.
ACCESSION   DAOMEO010000719 DAOMEO010000000
VERSION     DAOMEO010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6758_length_1795_cov_2.697701"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLH31_14495"
     CDS             <1..764
                     /locus_tag="PLH31_14495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQR90439.1"
                     /translation="QKAMYLSADHWRDNGRNDIEIEFHNAGPVLFGVKDYVPALMEYV
                     RKYRIDLNFGSRLVAVDGPARIATFAVTGADGEVRQARREFDFLHVCPPQTAPDFIRE
                     SPLAAESGWIEVDDASLRHKRFENVFGLGDACSAPNAKTAAAARKQAPVVAENVLSLL
                     DGQPMRAVYDGYGSCPLTVERGKIVLAEFGYGGKLLPSFPKWLLDGTKPTRLAWLLKS
                     EALPEIYWSAMLKGREWMAHPQHIEPAPQPRPAVA"
     gene            773..1567
                     /locus_tag="PLH31_14500"
     CDS             773..1567
                     /locus_tag="PLH31_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR90440.1"
                     /translation="MDLSPLQYGLGAGAGSLVGFTLGLVGGGGSILAVPLIVYLVGVK
                     DPHLAIGTSAFAVAANAFANLLNHARHGTVKWKIAGLFALAGVFGAFLGSSLGKAVDG
                     QKLLALFAALMLVVGVLMLRGRSASGDPDVVLTRGNAPKLVGAGALTGIMSGFFGIGG
                     GFLIVPGLMVSTRMPIYYAVGSSLVGVTAFGLTTAFNYALDGWVDWPLAAVFIGGGVL
                     GGLVGARLAKALSGQKGLLNTVFAGLIFVVAFYMLYRSAGALGLIG"
     gene            1577..>1795
                     /locus_tag="PLH31_14505"
     CDS             1577..>1795
                     /locus_tag="PLH31_14505"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQR90441.1"
                     /translation="MELDALILARMQFAFTVAFHIVFPAFSIGLASYLAVLEALWLKT
                     GRELYLNLFKYWLKIFAVAFGMGVVSGLV"
BASE COUNT          255 a          605 c          589 g          346 t
ORIGIN      
        1 cgcagaaggc catgtatctg tcggccgacc actggcgcga caacggccgc aacgacatcg
       61 agatcgaatt ccacaacgct ggcccggtgc tgttcggggt caaggactac gtcccggcgc
      121 tgatggaata tgtccgcaag tatcgcatcg acctgaactt cgggtcgaga ctggtcgcgg
      181 tggacggccc ggcgcggatc gcgaccttcg ccgtgacggg tgcggacggc gaggtgcgcc
      241 aggcgcggcg cgagttcgac ttcctgcacg tctgtccgcc gcagaccgcg ccggacttca
      301 ttcgcgaaag tccgctggcc gctgaaagcg gctggatcga ggtggacgac gccagcttgc
      361 gccacaagcg gttcgaaaac gtcttcggcc tgggcgatgc ctgctccgcc cccaacgcca
      421 agaccgctgc ggccgcccgc aaacaggcgc ccgtcgtggc cgagaacgtc ctgtcgctgc
      481 tggacggcca gccgatgcgg gcggtctatg acggctacgg ctcatgtccg ctcacggtcg
      541 agcgcggcaa gatcgtgctg gccgagttcg gctatggcgg caagctcctg cccagcttcc
      601 cgaaatggct tctggacggc accaagccga cccgcctggc ctggctgctg aagtccgagg
      661 cgctgccgga gatctactgg tccgccatgc tgaagggccg agaatggatg gcccaccccc
      721 agcacatcga gcccgcgcct caacctcgtc ccgccgtcgc ctgagcctga tcatggacct
      781 gtctccgctg cagtacggcc tcggcgccgg cgccggctcg ctcgtcggct tcaccctcgg
      841 cctggtcggc ggcggcgggt ccatcctggc cgtgccgctg atcgtctatc ttgtcggggt
      901 caaggatccg catctggcga tcggcaccag cgccttcgcc gtggccgcca acgccttcgc
      961 caatctgctc aaccacgcgc gccacggcac ggtgaaatgg aagatcgccg gcctgttcgc
     1021 cttggccggc gtcttcggcg cctttctcgg ctccagcctg ggcaaggcgg tggacggtca
     1081 gaagctgctg gctttgttcg ccgcgctgat gctggtggtc ggggtgctga tgctgagagg
     1141 ccgctcggcc tcgggggacc ccgacgtggt cctgacgcgg ggcaatgcgc cgaagctcgt
     1201 tggcgccggc gccctgacgg ggatcatgtc cggcttcttc ggcatcggcg gcggcttcct
     1261 gatcgtccca ggcctgatgg tctcgacccg catgcccatc tactatgcgg tcggttcgtc
     1321 gctggtcggc gtgacggcct tcggactcac caccgccttc aactacgccc tggacggctg
     1381 ggtcgactgg cccctggcgg ccgtcttcat tggcggcggt gtgcttgggg gtctggtcgg
     1441 cgcacggctc gcgaaagccc tgtcgggaca aaaaggccta ttgaacacag tgttcgccgg
     1501 gctgatcttc gtcgtcgcct tctatatgct ttaccgcagc gccggcgcgc tcggcctgat
     1561 cgggtgagac cacaccatgg aacttgacgc cctgatcctg gcgcggatgc agttcgcctt
     1621 caccgtcgcc ttccacatcg tctttccggc cttctccatt ggcctcgcca gctatctggc
     1681 cgtgctggag gccctgtggt tgaagacggg gcgagagctc tatctgaacc tgttcaaata
     1741 ctggctgaag atcttcgctg tggccttcgg catgggcgtc gtttcgggtc tggtc
//
LOCUS       DAOMEO010000720         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6759_length_1795_cov_2.695977, whole genome shotgun sequence.
ACCESSION   DAOMEO010000720 DAOMEO010000000
VERSION     DAOMEO010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6759_length_1795_cov_2.695977"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLH31_14510"
     CDS             <1..244
                     /locus_tag="PLH31_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQR90442.1"
                     /translation="VGLWSASEARRRSVVRKATPAVSGPSISEARSLLGVGPNATAEE
                     IKAAHARLIRLAHPDKGGTAGLAAQLNAARDRLLKG"
     assembly_gap    407..416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<417..1628)
                     /locus_tag="PLH31_14515"
     CDS             complement(<417..1628)
                     /locus_tag="PLH31_14515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQR90443.1"
                     /translation="MFAKLRAARTILATIGLVLSCVLAALVPAGSASAQIPYLQLNAA
                     EPKYAAIVIDANSGEVLYDKRADSPRYPASITKVMTVYLAFEALSEGRMKPTDRVVIS
                     PRAAAQAPTKLGLPPGDSLSVDEAIRILTVRSANDVAVAMAEKLAGSEARFAALMTLR
                     GQELGMRNTRFVNASGLPDSRQISTARDLAILSRATMRDFPQYYSYFSLKSVSFRGRT
                     IRGHNRLLDSMNGFDGLKTGYTNASGYNLAGSAVRDGRRLIAVVLGGSSTAWRDNNME
                     DLLLTGFDVLKRRAHGDRTTIAANLFESEPGGPIMRPSTEQGDGEQSGLKIVLTDSPR
                     AQPVKVSPTLKAAKAATKPAKKPKGEWAVQVGAFKSKGLASKQLTLVRGRFAKFVADA
                     EGAVEGAAGGAF"
BASE COUNT          299 a          556 c          630 g          300 t
ORIGIN      
        1 ggtcggcctg tggagcgcgt ccgaggcgcg tcgccgctcg gtagtccgca aggccacgcc
       61 agcggtttcc ggtccatcga tatccgaagc gcggtctctt ctgggcgtcg gccccaacgc
      121 gacggctgag gagatcaagg cggcccatgc gcgcctgatc cgtctggcgc atcccgacaa
      181 gggcggcacc gccggcctcg cggcgcagct caatgcggcg cgcgatcgat tgctgaaggg
      241 ctagacgcaa gaaaagagcc cagggcgcta ggcgctccgg gctcgataag atctgcgatt
      301 ttgtgccgag atcagcgcgg cgacaacacc atgcagggct ggcgcttggc cgtgatggcc
      361 gcgcaggtgc tgcgggcggc ttcagcggtc aggccctgga actgggnnnn nnnnnngaat
      421 gcgccgccgg ccgcgccctc gaccgcgccc tcggcgtcgg ccacgaactt ggcgaagcga
      481 ccgcgcacca gggtcaattg cttgctggcc agacccttgg acttgaaggc gccgacctga
      541 accgcccatt cgcccttggg ctttttggct ggcttggtcg cagccttggc ggccttgagg
      601 gtgggggaga cctttaccgg ctgggcgcgc ggcgagtcgg tcagcacgat cttcagaccc
      661 gactgctcgc cgtcgccctg ctcggtcgac ggccgcatga tcgggccacc gggctcgctc
      721 tcgaagaggt tggcggcgat cgttgtccgg tcgccatggg cgcgacgctt caggacgtcg
      781 aagcccgtca gcagcaggtc ttccatgttg ttgtcgcgcc aggcggtcga ggatccgccc
      841 aggaccacgg cgatcaggcg gcggccatcg cgaacggccg agccggccag attatagccc
      901 gaggcgttgg tgtagccggt cttcaggccg tcaaagccat tcatgctgtc gagcagacgg
      961 ttgtggcccc ggatcgtcct gccccggaac gaaaccgatt tcaggctgaa gtagctgtag
     1021 tactgcggga agtcgcgcat cgtggcgcgg gacaggatgg ccaggtcgcg ggcagtgctg
     1081 atctggcgac tgtcgggcag gcccgaagcg ttgacgaagc gggtgttgcg catgccaagc
     1141 tcctggccgc gcagggtcat cagggcggcg aaccgcgcct cgctgccggc cagcttttca
     1201 gccatggcca ccgccacgtc attggccgag cgcacggtca gaatacggat ggcctcgtcc
     1261 acgctgaggc tgtcaccggg cgggaggccc agcttggtcg gcgcctgagc ggcggcgcgg
     1321 gggctgatga cgacccggtc ggtcggcttc atgcgtcctt cgctcagcgc ttcgaaggcg
     1381 agatagaccg tcatgacctt ggtgatggac gcaggatagc gggggctgtc ggcgcgcttg
     1441 tcgtagagga cctcgcccga attggcgtcg atcacgatgg ccgcatattt cggctcggcg
     1501 gcgttcagct ggagataggg tatctgggcc gaggcgctgc cagcgggcac cagggcggcc
     1561 aggacacacg aaagcaccag gccaatagtg gcgagaatgg tacgggcggc gcgaagcttg
     1621 gcgaacatga atgagcgtcc gtcacatttg aactgagcgg ttccgcaccg ccacacgcgt
     1681 gaccctagca tgacccgacc cgcctttagc caaagtaaac aaccggtgag cgatgttgtc
     1741 gcagatgcga aaaaaacgtg cgcgttgcgg tcaggcttca gcatgaccct aggtc
//
LOCUS       DAOMEO010000721         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6771_length_1792_cov_2.860679, whole genome shotgun sequence.
ACCESSION   DAOMEO010000721 DAOMEO010000000
VERSION     DAOMEO010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_1792_cov_2.860679"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..87
                     /locus_tag="PLH31_14520"
                     /pseudo
     CDS             1..87
                     /locus_tag="PLH31_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
     gene            complement(203..>1792)
                     /locus_tag="PLH31_14525"
     CDS             complement(203..>1792)
                     /locus_tag="PLH31_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HQR90444.1"
                     /translation="GGEGWRRITYADAKTKIEAVAGGLKALGLGPGKPLLILARNGID
                     HALISYAAMSLGAPIAPVSPQYGLAGAELSRLQFAVERLKPAAVYADDAIAFGGALAA
                     PFLAGLPVIASLNPRDGDIPFADLLEAPPLTPLCQPGDVAKLLLTSGSTGKPKAVICT
                     HANIALNAAQIEACYADPEPPVLVNSAPWSHSLGANAILHMVLHRGGTLYIDAGQPTP
                     TRFSETLRNLREVATTYHNMVPAGWGLLVGELERDAALAARFFEKVRVLQYGGASMAQ
                     SILDRVQAVAVRTMGERVTFAAGYGATETGPTACNIHWLNARSGMVGLPTPGTAVKLV
                     PAGDKFEIRVKGPQVSPGYLDQPEATRDAFDEEGFYRLGDAARLADPEDPAAGLVFDG
                     RLVENFKLASGTFVAAGALRVAAVSAIGGVVSDAVVCGEGRDGVGLMLFVDPTAAARL
                     GDASAVRAAVVEGLERLNAAAKGGGKVARALILDGAPDAASGELTDKGYINQALARDR
                     RLAELERLFAEQPDDGVMVFR"
BASE COUNT          277 a          651 c          601 g          263 t
ORIGIN      
        1 gccggcccgc tgccggacaa ctttgtcacc ggactgggcg tgtgggggct gtactatttc
       61 gggcagctgc tgatctgcgt gggggtgaca aacaccttgt catcccgggc gtagcgaagc
      121 gaagacccgg gaccgatctg gaacacggtg ttcaggcgtc ggtcccggat cggcgcgcta
      181 cgcgcttgtc cgggatgaca gttcagcgaa acaccatcac cccatcatcc ggctgctcgg
      241 caaacagccg ctccagttcc gccagccgcc ggtcccgcgc cagggcctgg ttgatatagc
      301 ccttgtcggt cagctcgccg ctagccgcgt ccggcgcgcc gtccaggatg agcgcccgcg
      361 ccaccttgcc gcctcccttg gccgcagcgt tcagacgttc cagcccttcg accaccgcag
      421 cccgcaccgc cgaggcatcg cccaaccgcg cggcggcggt gggatcgacg aacagcatca
      481 gccccacgcc gtcacgcccc tcgccgcaga ccaccgcgtc ggagaccacg ccgccaatgg
      541 ccgacaccgc cgcaacccgc agggcgccgg ccgcgacgaa ggtgccgctg gccagcttga
      601 aattctcgac cagccggccg tcgaacacca ggcccgccgc cggatcttcc ggatcggcca
      661 gccgcgcggc gtcacccagg cggtagaagc cttcctcgtc aaaggcgtcg cgggtggctt
      721 caggctggtc cagatagccg ggcgagacct gcgggccctt gacccggatc tcgaacttgt
      781 cgccggccgg gaccagcttc accgccgtgc cgggcgtcgg caggcccacc atgcccgagc
      841 gggcgttcag ccagtggatg ttgcaggcgg tggggccggt ctcggtggcc ccatagccgg
      901 cggcgaaggt cacccgctca cccatggtcc gcaccgccac ggcctggacc cggtcgagga
      961 tcgactgggc catcgaggcg ccgccatatt gtagcacacg gaccttctcg aagaacctgg
     1021 ccgccagggc cgcgtcgcgc tccagctcgc ccaccagcag gccccagccg gccgggacca
     1081 tgttgtgata ggtcgtcgcc acctcgcgca ggtttcgcag ggtctcggaa aagcgcgtcg
     1141 gcgtgggctg gccggcgtcg atatacaggg ttccgccccg gtgcagcacc atgtgcagga
     1201 tggcgttcgc ccccaggctg tggctccacg gcgcggaatt gaccagcacc ggcggctcgg
     1261 ggtcggcgta gcaggcctcg atctgggcgg cgttcagcgc gatattggcg tgggtgcaga
     1321 tcacggcttt cggcttgccg gtcgagcccg aggtcagcag cagcttggcc acatcgccgg
     1381 gctggcagag gggcgtcagc ggcggcgcct ccagaagatc cgcaaacgga atatctccat
     1441 cgcgcgggtt caggctggcg atcaccggca gccccgccag gaacggcgcg gccagagccc
     1501 cgccgaaggc gatagcgtcg tcggcataga ccgccgccgg tttcagccgc tcgacggcga
     1561 actgcaggcg cgaaagctcg gctccggcca ggccatactg cggcgacacc ggcgcgatcg
     1621 gcgcgcccag gctcatggcg gcatagctga tcagggcgtg gtcgatgccg ttgcgggcca
     1681 agatcagcag cggcttgccc ggaccaagac ccagcgcctt cagcccgccg gccacggctt
     1741 cgatcttcgt cttggcgtcg gcgtaggtga tcctccgcca gccttcgccc cc
//
LOCUS       DAOMEO010000722         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6780_length_1791_cov_2.147465, whole genome shotgun sequence.
ACCESSION   DAOMEO010000722 DAOMEO010000000
VERSION     DAOMEO010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_1791_cov_2.147465"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLH31_14530"
     CDS             complement(<1..764)
                     /locus_tag="PLH31_14530"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516311.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQR90445.1"
                     /translation="MSAAPDPVVIVSFARTPMGGFQGALGGVKATDLGAAAVKAAIER
                     AGVSGDLVEQIIMGCVLPAGLGQAPARQAAVGAGLPLSVEATTVNKMCGSGMQAAIMA
                     HDALAAGSAEIIVAGGMESMTGAPYLMNKHRGGARIGHDQMWDSMYLDGLEDAYTPGK
                     LMGAFAEDSAATYQFSREAMDEYATKGLMKAKAAIQSGAFKAEITPVTVITRKGAEIV
                     EQDEQPLKADPAKIPTLRPAFSRDGGVTAANSSSIS"
     gene            987..>1256
                     /locus_tag="PLH31_14535"
     CDS             987..>1256
                     /locus_tag="PLH31_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90446.1"
                     /translation="MGRTADYTNERCSIAATLEVVGDPWTLLILRDAFAGVKRFEQWQ
                     ERLGVARNVLASRLKSLVAHGVMEAQRYSERPPRQEYVLTPKGLDL"
     assembly_gap    1257..1266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1267..1494
                     /locus_tag="PLH31_14540"
     CDS             <1267..1494
                     /locus_tag="PLH31_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR90447.1"
                     /translation="SPLLLTMAEWGDQHVYGIGKGPVHFVHKTCGCDFHPRLACEACG
                     EVIDRRDLRRVVHETSQTVGEALEAVKIPAE"
     gene            1600..>1791
                     /locus_tag="PLH31_14545"
     CDS             1600..>1791
                     /locus_tag="PLH31_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90448.1"
                     /translation="MALLKTVAGIFAIRRDRPELLIAQATAFSRQVPMMYLVVLVNAI
                     ALAVTHFQSAPRVLTLGGPT"
BASE COUNT          295 a          579 c          584 g          323 t
ORIGIN      
        1 tcgctgatcg agctggaatt ggccgcggtg accccgccgt cgcggctgaa ggcgggccgt
       61 agcgtcggga tcttggccgg atcggccttc agaggttgtt cgtcctgctc gacgatttcg
      121 gcgcccttgc gggtgatgac ggtgaccggg gtgatctcgg ccttgaaagc gccgctctgg
      181 atcgccgcct tggccttcat caggcccttg gtcgcgtact cgtccatggc ttcgcggctg
      241 aactgatagg tcgcggccga gtcctcggcg aaggcgccca tcagcttgcc gggggtatag
      301 gcgtcttcca ggccgtccag gtacatcgag tcccacatct ggtcgtggcc gatgcgggcc
      361 ccgccccggt gcttgttcat caggtagggc gcgccggtca tgctctccat gccgccggcc
      421 acgatgattt cggcgctgcc ggccgccagg gcgtcgtggg ccatgatcgc cgcctgcatg
      481 ccgctgccac acatcttgtt gaccgtggtg gcctcaaccg acaggggcag gccggcgccg
      541 actgccgcct gacgcgcggg cgcctggcca aggccggcgg ggagcacaca gcccatgatg
      601 atctgctcga ccagatcgcc cgaaacaccc gcacgttcga tggcagcctt caccgccgct
      661 gcgcccagat cggtcgcctt gaccccgccc agcgcgccct ggaagccgcc catcggcgtg
      721 cgggcgaacg agacgatgac gacaggatca ggggcggcgg acatggacga tctccggatg
      781 gaaagaattc gggtggaata ggccccgctg cggtggcttt gcgcaagcag tttgcggaaa
      841 ggggaggcga tcgcaatgat gcagcagcgg ccatcgcctc ccaaggcgca accgagagag
      901 tcagttgtga taaaaaacct aatgccgttc atcctcaaac gcaacggtct cttgctttcc
      961 ctatcggaac gcctcacctt cgcttcatgg gccgcaccgc cgactacacg aacgaacgct
     1021 gcagcattgc cgccaccctc gaggtggtgg gtgacccatg gacccttctg atccttcgtg
     1081 acgccttcgc cggggtgaag cgctttgagc aatggcagga acgcctgggc gtcgcccgca
     1141 atgtcctggc gtcgcggctc aaatccctgg tcgcgcacgg cgtgatggag gcgcagcgtt
     1201 actccgagcg cccgccacgc caggaatatg tgctcacgcc caagggactg gatctcnnnn
     1261 nnnnnnagcc cgttgctgtt gaccatggcc gaatggggcg atcagcacgt ctatggcatc
     1321 ggaaaggggc cggtgcattt cgtgcacaag acttgcggct gcgacttcca tccgcgcttg
     1381 gcctgcgagg cttgcggcga ggtgatcgat cgccgggacc tgcgccgcgt ggttcacgag
     1441 acctcgcaga cggtcggcga ggccctcgaa gcggtgaaga tccctgccga ataggccatg
     1501 ggcgcgcagg tcaatatgcc gcacctggcg cccgaagcca gcttaccgaa tatttagggc
     1561 ttgccccgca ccgtgatcgg aagccgaggg caggcataga tggcgctatt gaagaccgtc
     1621 gcggggatct tcgcgatccg cagggaccgt ccggaacttc tgatcgctca ggcgacggcg
     1681 ttctcgcgcc aggtgccgat gatgtatctg gtcgtcctgg tcaatgcgat cgcgctggcc
     1741 gtcacccatt tccagtccgc accgcgtgtc ctgaccctgg gcggacccac g
//
LOCUS       DAOMEO010000723         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6789_length_1789_cov_2.303345, whole genome shotgun sequence.
ACCESSION   DAOMEO010000723 DAOMEO010000000
VERSION     DAOMEO010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_1789_cov_2.303345"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLH31_14550"
     CDS             <1..468
                     /locus_tag="PLH31_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HQR90449.1"
                     /translation="GGTLICQKDSFLAAARGVSIGIHFQKRMMTGLFGGEGFIMQKLE
                     GDGWVFVQMGGTLVERELKAGEELHVDTGCLAAYTPGVDFDLVMAGGVKSVLFGGEGL
                     FFARLRGPGKVWIQSLPFSRLAGRMLQAAGGQGGNQGEGSLLGGLGDLIGGNR"
     gene            complement(494..823)
                     /locus_tag="PLH31_14555"
     CDS             complement(494..823)
                     /locus_tag="PLH31_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQR90450.1"
                     /translation="MVKTNIEVGERRVPDDCQTMLDVRQGVDALDRALVALLTERQGY
                     MDAAARIKADRSKVFDRARIEDVVTKVKAAAREAGLSEAIVEPVWRTLIDRCIAYEYD
                     SWDRTKG"
     gene            complement(830..1786)
                     /gene="gcvA"
                     /locus_tag="PLH31_14560"
     CDS             complement(830..1786)
                     /gene="gcvA"
                     /locus_tag="PLH31_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HQR90451.1"
                     /translation="MERQNERRRLPPLNALRAFEAAARHLNFSRAADELSVTPGAVSQ
                     QIQNLEDYVGAALFKRTPKGLLLTDAAQTALPALREAFDRLAEAASLLTAAVDGRRLT
                     LTAAPSFAAKWLVPRLGKFEQAQPQVDVWLSAGMEVVDFAAGEVDLAIRYGSGRYPGL
                     EVVRLLSETVVPVVSPALLADNPLTAPEDLARHILLHDGSPDADDSCPDWAMWLAARG
                     LRTIDGSRGPRFNQSSMVIEAAANGRGVALAKRTLAQADLDAGRLVIPFEMATAVDFA
                     YYVVHPKAKGRLPQVKAFVTWLKDEAAAHEAALLTMDNGAGI"
BASE COUNT          274 a          574 c          649 g          292 t
ORIGIN      
        1 ggtggaaccc tgatctgcca gaaggacagc ttcctggctg ccgcgcgcgg cgtgtcgatc
       61 ggcatccact tccagaagcg gatgatgacc ggcctgttcg gcggcgaagg cttcatcatg
      121 cagaagctgg aaggcgacgg ctgggtcttc gtccagatgg gcggcacgct ggtcgagcgc
      181 gagctcaagg ccggcgagga gctgcacgtc gataccggct gtctggccgc ttatacgccg
      241 ggcgtcgact tcgacctggt gatggccggc ggggtcaaga gcgtgctgtt cggcggcgag
      301 gggctgttct tcgcgcgcct gcgcggcccg ggaaaggtgt ggatccagtc gctgccgttc
      361 tcgcgtctgg caggccggat gctgcaggcg gccggcggcc agggcggcaa ccagggcgag
      421 ggctcgctgc tgggcggtct gggcgacctg atcggcggca atcgctagaa tcagcagggc
      481 tgaggttcgc gcctcagccc ttcgtcctgt cccagctgtc gtactcatag gcgatgcagc
      541 ggtcgatcag ggtgcgccag accggctcga cgatggcttc cgacaagccg gcctcgcggg
      601 cggcggcctt caccttggtc acgacgtcct cgatccttgc gcggtcgaag accttggagc
      661 ggtcggcctt gatgcgcgcg gcggcgtcca tatagccctg acgctcggtc agcagggcca
      721 ccagggcgcg gtccagggcg tccacgccct gacgcacgtc aagcatggtc tggcagtcgt
      781 cgggaacccg tcgttcgccg acctcgatat tcgtcttgac catgaagccc tagatacccg
      841 cgccgttgtc catcgtgagg agcgccgcct cgtgcgcggc ggcctcgtcc ttgagccagg
      901 tgacgaaggc cttcacttga ggcagtcgtc ccttggcctt gggatggacg acgtaatagg
      961 caaagtcgac ggccgtggcc atctcgaagg ggatcaccag gcgtccggcg tccagatcgg
     1021 cctgagccag ggtgcgtttg gccaaggcta cgccacggcc gttggcggcg gcctcgatca
     1081 ccatgctgga ctggttgaag cgcggacccc gtgagccgtc gatggtgcgc aggccgcgcg
     1141 ccgccagcca catggcccag tcggggcagc tgtcatcggc gtcgggcgag ccgtcgtgta
     1201 gcaggatgtg gcgggccagg tcctcgggcg cggtcagcgg attatcagcc aacagggcgg
     1261 ggctgaccac gggcacgacg gtttcgctca gcaggcgcac cacctccagg ccgggatagc
     1321 ggccggagcc atagcgaatc gccaggtcca cctcgccggc cgcgaaatcg accacctcca
     1381 tgccggccga cagccagaca tccacctggg gctgggcctg ctcgaacttg cccaggcgcg
     1441 gcaccagcca cttggcggcg aacgacggcg cggcggtcag ggtcagtcgg cgaccgtcga
     1501 cggcggcggt gagaagggat gccgcctcgg ccaggcgatc gaaggcctcg cgcagggcgg
     1561 gcagggcggt ctgggccgcg tcggtcagca acaacccctt gggcgtgcgc ttgaacaggg
     1621 ccgcgccgac atagtcttcc aggttctgga tctgctggct gaccgcgccg ggcgtgaccg
     1681 acagctcgtc ggcggcgcgg ctgaagttca gatggcgggc tgcagcctcg aatgcccgaa
     1741 gggcattcaa cggaggaagc ctgcggcgct cgttctgtct ttccatgag
//
LOCUS       DAOMEO010000724         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6790_length_1789_cov_2.278547, whole genome shotgun sequence.
ACCESSION   DAOMEO010000724 DAOMEO010000000
VERSION     DAOMEO010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6790_length_1789_cov_2.278547"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLH31_14565"
     CDS             <1..140
                     /locus_tag="PLH31_14565"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQR90452.1"
                     /translation="GVLTVDEEDQAWTRAKVSEGDRGGAAAKACLETVALKRQLLGQT
                     R"
     gene            137..598
                     /gene="nusB"
                     /locus_tag="PLH31_14570"
     CDS             137..598
                     /gene="nusB"
                     /locus_tag="PLH31_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQR90453.1"
                     /translation="MSKNNRIQPRSVARLAAVQALYQMEVSGVGVDAVVREFSEHRFD
                     RDVDGPDGDQLAAADETFFAELAKGVVDKQGEVDQAIVKRLASGWRLDRLDATARAVL
                     RAGAFELMHRPDVPTEVVIDEYVEIAKSFFEGPEAGFINGALDAIARDART"
     gene            558..1622
                     /gene="thiL"
                     /locus_tag="PLH31_14575"
     CDS             558..1622
                     /gene="thiL"
                     /locus_tag="PLH31_14575"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQR90454.1"
                     /translation="MAPSTRSPAMHEPDADDDWFADEPPSETAERPAANEFDHIARLL
                     RPLTRGDPVALDLLDDAAVLPSRIDHDLVITKDAMVAGVHFLAGEDLDLVAKRLLRTN
                     LSDLAAKAAEPYGYFLAVGWPSGTTIEDRETFARGLAEDGELYDINLLGGDTVSTSGA
                     LVVSATFLGWVPVGDAVLRRGARVGDRLMVSGTIGDGWLGLLAHWGEVEDPDGSLVSS
                     YRLPPPRLSIRDALRAYARAAADVSDGLLADAAHVAKASGLRVLVDLDRLPLSPGARN
                     WLSAQPEAGEARMSLASGGDDYEIVCAVDPTDVAAFQAAAMASGVPVRDIGEFVEGEG
                     VCALFKGKDITPERLGWLHG"
BASE COUNT          271 a          606 c          617 g          295 t
ORIGIN      
        1 ttggcgtgct gaccgtcgat gaggaagacc aggcctggac ccgcgccaag gtcagtgaag
       61 gcgatcgcgg cggcgctgcg gccaaggcct gcctggaaac cgtcgccctg aagcgtcaat
      121 tgctggggca gacccgatga gcaagaacaa ccgcatccag ccccgctcgg tggcccgtct
      181 ggccgccgtc caggccctgt accagatgga agtctcgggc gtgggcgtcg acgctgtggt
      241 tcgcgagttc tccgaacacc gcttcgatcg tgacgtggac ggccccgacg gcgaccagct
      301 ggccgccgcc gacgagacct tcttcgccga gcttgccaag ggcgttgtgg acaagcaggg
      361 cgaggtcgat caagccatcg tcaagcgcct ggcctcgggc tggcgcctgg atcgtctgga
      421 cgcaaccgcc cgcgctgtgc tgcgagccgg cgcgttcgag ctgatgcatc gaccggacgt
      481 gccgaccgag gtcgtcatcg acgagtatgt cgagatcgcc aagtcgttct tcgaaggacc
      541 ggaggctggt ttcatcaatg gcgccctcga cgcgatcgcc cgcgatgcac gaacctgacg
      601 ccgacgacga ctggttcgcc gacgagccgc cgtccgaaac ggcggagcgg ccggctgcca
      661 atgagttcga ccacatagcg cgcctgcttc ggccgttgac gcgcggcgat ccggtcgcgc
      721 tggacctgct ggatgacgcg gcggtgctcc cctcgcgcat cgaccatgac ttggtgatca
      781 ccaaggacgc tatggtcgcc ggggtgcatt tcctggctgg cgaagacctg gaccttgtcg
      841 ccaagcggtt gctgcgcacc aatctgtcag acttggcggc caaggcggcc gaaccctacg
      901 gctacttcct ggcggtgggc tggccctcgg gcaccaccat cgaggatcgc gagacctttg
      961 cgcgtgggct ggccgaggac ggcgagcttt atgacatcaa cctgctgggc ggcgacaccg
     1021 tcagcacctc cggcgcccta gtcgtttcgg cgactttcct gggctgggtc cccgtcggcg
     1081 acgcggtgct tcggcgcggc gccagggtcg gcgaccgcct gatggtcagc gggactatcg
     1141 gcgacggctg gctgggcctg ctggcccact ggggcgaagt ggaggatccg gatggcagcc
     1201 tggtgagcag ctatcggctg ccgccgccgc gcctgagcat ccgcgacgcc ctgcgagcct
     1261 atgcccgcgc cgccgccgac gtctcagacg gcctgctggc cgacgccgcc cacgtggcca
     1321 aggccagcgg tctgcgcgtg ctggtcgacc ttgaccgctt gccgctgtcc cccggagccc
     1381 gcaactggct cagcgcccag ccggaagccg gcgaggcgcg gatgtctctg gcttcgggcg
     1441 gcgacgacta cgagatcgtt tgcgccgtcg atcctaccga tgtggccgcc ttccaggccg
     1501 ccgccatggc ctcgggcgtg cccgtccgcg acatcggcga gtttgtggaa ggcgagggtg
     1561 tctgcgccct gttcaagggc aaggacatca cgccggagcg tctcggctgg ctccacggct
     1621 aggcggtagc aaaacttcaa ggcttggctg gtatggaagg gcgcatgacc gctccattag
     1681 ccttcacgcg ccgcttcctg gtcaccgccg ccttgctggc cgtggccggt cccgccttgg
     1741 cgagcggacc gcccaagaaa aaagaaggcg aggccgctgc gcctgatcc
//
LOCUS       DAOMEO010000725         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6795_length_1788_cov_3.141950, whole genome shotgun sequence.
ACCESSION   DAOMEO010000725 DAOMEO010000000
VERSION     DAOMEO010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6795_length_1788_cov_3.141950"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1707)
                     /gene="ggt"
                     /locus_tag="PLH31_14580"
     CDS             complement(31..1707)
                     /gene="ggt"
                     /locus_tag="PLH31_14580"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQR90455.1"
                     /translation="MRLFVRFRAVLAALLLVAAPALASPRPVLTAGAVASPDRYGALA
                     AEEILKAGGNAVDAAVATAFALAVTYPEAGNLGGGGFMTLQVDGKPYFLDYRETAPAA
                     STADMFLGPDGEPVAERSMYGNLAVATPGTVRGMAMAHQRFGKLPWARVLAPSIRYAR
                     DGFLVTPQLIGILDGSRAPLAKTNFEAHFGGLKAGEMFRQPALAEVLTRIATDGDKAF
                     YEGRTADLLVAQMDRGPIKGLITKADLAAYKAVWREPLQADWRGYNVITAPPPSSGGI
                     ALMQLLLMKQEAGKLFAGVPLNSPQYMHLVSEIEKRVFADRAEYLGDPAFVTVPVQAL
                     IAPDYMARRAAGIDPDKPSPTSSVKPGLERPQTTHFSIVDKWGNAVSNTYTINGWFGS
                     GVVVEGAGFLLNNEMDDFSAKPGAPNMFGVVGGEANAITPGKRPLSSMTPAILTKDGQ
                     VVMVIGTPGGSRIFTSVFQVLAAVYDHGLSLADAQKAQRFHHQLLPKDTIFFEPYAPP
                     SEALKSALEARGYVIEMQDYNGDIEAIQVVGRTPVPEADPRARGVALVVK"
BASE COUNT          298 a          620 c          600 g          270 t
ORIGIN      
        1 tctgggccac ctcccccaga gggggaggag ctacttcacc accagcgcca caccccgcgc
       61 ccggggatca gcctccggaa ccggcgtccg gcccaccacc tgaatcgcct cgatgtcgcc
      121 attgtagtcc tgcatctcga tcacatagcc gcgcgcttcc agcgccgact tcagcgcctc
      181 cgacggcggg gcatagggct cgaagaagat cgtgtccttg ggcagaagct ggtggtgaaa
      241 gcgctgggcc ttctgggcgt cggccagcga caggccgtgg tcatagacgg cggccagaac
      301 ctggaacacc gacgtgaaga tccgcgagcc gcctggcgtg ccgatcacca tcaccacctg
      361 gccatccttg gtcagaatgg ccggggtcat cgacgacagc ggccgcttgc ccggcgtgat
      421 ggcgtttgcc tctccgccga cgacgccaaa catgttcggc gcgcccggct tggccgagaa
      481 gtcgtccatc tcgttgttga gcaggaagcc cgccccctcg accaccacgc ccgacccgaa
      541 ccagccattg atcgtatagg tgttggagac ggcgttgccc catttatcga caatcgagaa
      601 atgggtggtc tgcggccgct ccagcccggg cttgaccgag gaggtcggcg agggcttgtc
      661 cggatcgatc ccagcggcgc ggcgggccat atagtccggc gcgatcaggg cctggaccgg
      721 caccgtcacg aacgccggat cgcccagata ttcggcccgg tcggcgaaga cccgcttttc
      781 gatctccgac accagatgca tgtactgcgg gctgttcagc gggacgcccg cgaacagctt
      841 gccggcctcc tgcttcatca gcagcaactg catcagggcg atcccgcccg agctgggcgg
      901 cggggcggtg atcacgttgt agccgcgcca gtccgcctgc aggggctcgc gccagacggc
      961 cttgtaagcc gccagatcgg ccttggtgat caggcccttg atcggcccgc gatccatctg
     1021 ggccaccagc aggtcggcgg tccttccctc atagaaggcc ttgtcgccat ccgtcgcgat
     1081 gcgggtcaga acctcggcca gggccggctg gcggaacatc tcgcccgcct tcagtccccc
     1141 gaagtgggcc tcgaaattgg tcttggccag cggcgcgcgg ctgccgtcca ggatgccgat
     1201 cagctgcggc gtgaccagga agccgtcgcg ggcgtagcgg atcgacggcg ccagcaccct
     1261 cgcccagggc agcttgccaa agcgctggtg agccatggcc atgccgcgca cagtgcccgg
     1321 ggtggcgacg gccagattgc cgtacatcga ccgctcggcc accggctcgc cgtctgggcc
     1381 caggaacatg tcggccgtcg acgcagccgg agcggtctcg cggtagtcga ggaagtaggg
     1441 cttgccgtcg acctgcaggg tcatgaatcc gccgccgccc agattgccgg cctcgggata
     1501 ggtgaccgcc agcgcaaaag ccgtggccac cgccgcatcg acggcgttgc cgccggcttt
     1561 gaggatctcc tcggcggcca gggcgccata gcggtcgggc gaggccaccg cgcccgccgt
     1621 caggaccggc ctggggctgg ccagggccgg cgcggcgacc agcagcaggg cagcgaggac
     1681 cgcgcgaaaa cggacgaaca gacgcaagcg acggcctcct tcaaactgaa gggctcacgt
     1741 tagccgatca cggcggcggt agaagcctgg ccttgcgccg gcggtcag
//
LOCUS       DAOMEO010000726         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6800_length_1788_cov_2.155222, whole genome shotgun sequence.
ACCESSION   DAOMEO010000726 DAOMEO010000000
VERSION     DAOMEO010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6800_length_1788_cov_2.155222"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>359)
                     /locus_tag="PLH31_14585"
     CDS             complement(<1..>359)
                     /locus_tag="PLH31_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90456.1"
                     /translation="GKAGELGWFGGNKSAVASAPVKGQAWSLFGKPRSANAPRRGIPK
                     PEGFAVWQSRIDSSGADPMACVRMSKALDPSKPYADYVLISPDTGRTPAVRVKGDELC
                     VGGVGFTDHRVTLLKGL"
     assembly_gap    360..369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(546..1352)
                     /locus_tag="PLH31_14590"
     CDS             complement(546..1352)
                     /locus_tag="PLH31_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQR90457.1"
                     /translation="MVSRRLLLGVLASGLAGAPLAALAEGDDHHAPAPRKAAPKPRAA
                     AARAAPAPVRSTVAEYPKPVETHAPEVIINPDEALGRLKHGNAIFARGGASMALPSWT
                     RINELAGGQKPFAIIVGCSDSRVPPELVFDCTLGELFVVRVAGSTVSREGLGSILYAV
                     EHLGAPLIVVMGHTKCGAVGAAVDVVTKHSHLHGALFDMALPIVPAVLEAQAKSPVDL
                     QDAAIRQNVHDTAERLKSADHILSERIASGRLKIVSACYSLSTAQVEFNV"
     gene            complement(1435..>1788)
                     /locus_tag="PLH31_14595"
     CDS             complement(1435..>1788)
                     /locus_tag="PLH31_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR90458.1"
                     /translation="TRGRIGVALGGGVISRYAAVIDERAVGLPLSVFVTVTLSSQAEA
                     TLSAFEKAVANVREVAECYLMTGGSDYLLRLVVRDVDDLERVHAQELTRIPGVVRVSS
                     SIALRAVVKRAELPL"
BASE COUNT          314 a          601 c          594 g          269 t
ORIGIN      
        1 gcaggccctt gagcagggtg acgcgatgat cggtgaagcc gaccccgccc acacacagct
       61 cgtcgccctt gacccgcacg gccggcgtgc ggccggtgtc gggcgagatc agcacatagt
      121 ctgcataggg cttggaggga tccagcgcct tgctcatccg cacgcaggcc atggggtcgg
      181 cgccgctgct gtcgatgcgg ctttgccaga cggcgaagcc ttcgggcttg ggaatgccgc
      241 gacgcggcgc attggccgag cgaggcttgc caaacagcga ccaggcctgg cccttgaccg
      301 gcgccgaggc caccgcgctc ttgtttccgc caaaccagcc cagctcgccg gccttgcccn
      361 nnnnnnnnng ccttcagccg gtcaagaaac gccgcgcggg cgccgggagt cgagtcgtcc
      421 gaaccgctcg cgggcggtgt ctcgtccgtc gacatgcagc atcccccggt tcaccacgtt
      481 cagaggggga tgtgacgcca aggcccgcgc cgggtcaacg ggcgggagcc cgttgtcgca
      541 caagcttata cgttgaactc gacctgagcc gtcgacagac tgtagcaggc cgaaacgatc
      601 ttcaggcggc ccgaggcgat acgctccgac agaatgtgat cggcggactt caggcgctcg
      661 gccgtgtcgt gaacattctg acggatcgcg gcgtcctgca gatcgacggg ggacttggcc
      721 tgagcctcca gaacggccgg gacgatcggc agggccatgt cgaacagcgc gccatgcaga
      781 tggctgtgct tggtgaccac gtccaccgcc gccccgacgg cgccgcactt ggtgtggccc
      841 atcacgacga tcagcggcgc gcccaggtgc tcgacggcat agaggatcga gcccagaccc
      901 tcgcggctga cggtcgagcc ggccacgcgg accacgaaca gctcgcccag ggtgcagtcg
      961 aacaccagtt ccggcggcac gcggctgtcg gagcagccca cgatgatggc gaagggcttc
     1021 tggccgccag ccagctcatt gatccgcgtc cagctgggca gggccatgct ggcgccgccg
     1081 cgcgcgaaga tggcgttgcc gtgcttgagg cggcccagcg cttcatcggg attgatgatc
     1141 acttcgggcg catgggtctc gaccggcttg ggatattcgg cgaccgtcga gcgcaccggc
     1201 gcgggcgcgg cgcgggctgc agcggcacgg ggcttgggcg cggccttgcg cggcgcgggc
     1261 gcgtgatgat catcaccctc ggccagggcg gccagcggcg cgccagccag tccggacgcc
     1321 agaacgccca gtagaagtct tctcgaaacc atctcgtccc ccgacgtgga ttttcgcgtc
     1381 gggacaaagg actagagaga caaggttaac cgaccgtagg tatttgaagc actgtcacag
     1441 cggcaattca gcgcgcttga ccaccgcgcg cagggcgatg ctggaactga ccctgacaac
     1501 gcctggaatt cgcgtcagct cctgggcgtg aacgcgctcc agatcatcga catcccgcac
     1561 caccagccgc agcagatagt ccgatccgcc ggtcatcaga tagcattcgg ccacctcgcg
     1621 cacattggcg acggccttct cgaaggcgct cagcgtcgcc tcggcctggg acgacagggt
     1681 gacggtgaca aagacgctca gcggcaggcc taccgcccgc tcatcgatga cggcggcata
     1741 gcgactgatg accccgcctc ctagagctac accaatacga ccacgggt
//
LOCUS       DAOMEO010000727         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6801_length_1788_cov_2.111368, whole genome shotgun sequence.
ACCESSION   DAOMEO010000727 DAOMEO010000000
VERSION     DAOMEO010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6801_length_1788_cov_2.111368"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /gene="rplB"
                     /locus_tag="PLH31_14600"
     CDS             complement(<1..629)
                     /gene="rplB"
                     /locus_tag="PLH31_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQR90459.1"
                     /translation="MALKHFNPASPGQRGLVLIDRSELYKGRPEKKLVEGLTKSGGRG
                     GNGRIAVRFRGGGAKRLYRLVDFKRRKQGVASVVRIEYDPNRTAFIALIKYQTDGELA
                     YILAPQRLKVGDEVVTADKVDVKPGNTSPLRTLPIGTIIHNVELKPAKGGQIARSAGA
                     YAQLVGRDAGYAQIRLNSGELRMVLDTCLATVGAVSNPDHMNENLGKAGR"
     gene            complement(634..930)
                     /locus_tag="PLH31_14605"
     CDS             complement(634..930)
                     /locus_tag="PLH31_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQR90460.1"
                     /translation="MAATARHYDTILSPVITEKATLLSEQNKVVFRVSADASKDEIAA
                     AVEELFKVKVTKVNTMVTKGKTKRFRGIIGRRSDVKKAIVTLAEGQSIDITTGL"
     gene            complement(930..1568)
                     /gene="rplD"
                     /locus_tag="PLH31_14610"
     CDS             complement(930..1568)
                     /gene="rplD"
                     /locus_tag="PLH31_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQR90461.1"
                     /translation="MKLDVIKLDGGKAGSVDLDDAIFGIADIRGDILQRVVTWQLAKR
                     RSGNHKIQVRNEVSRTGKKMYKQKGTGGARHGSRRAAQFVGGAKAHGPVVRSHAFDLP
                     KKIRAMALRHALSSKAKAGSLIVLDNVALTDPKTAALRATFEKMGLKNALVIAGPEVD
                     GNFKLAARNIPNIDVLPNAGLNVYDVLRRHTLVLTKDAVDAISARFAEKEAA"
     gene            complement(1568..>1788)
                     /locus_tag="PLH31_14615"
     CDS             complement(1568..>1788)
                     /locus_tag="PLH31_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQR90462.1"
                     /translation="KVDVERGLILVKGAVPGHEGSYVKVRDAIKKAAPADLPRPGAFR
                     KAGQAPATAAAETPAEEAPAASTEEGEG"
BASE COUNT          289 a          542 c          605 g          352 t
ORIGIN      
        1 cgaccggcct tgccgaggtt ttcgttcatg tggtcagggt tggagactgc gccgaccgtg
       61 gccaggcagg tgtcgaggac catgcgcagc tcgcccgagt tcaggcggat ctgggcgtag
      121 ccggcgtcac gaccgaccag ctgggcatag gcgccagccg aacgggcgat ctgaccaccc
      181 ttggcgggct tcagctcgac attgtggatg atcgtgccaa tcggcagggt ccgcagcggc
      241 gaggtgttgc ccggcttcac gtcgaccttg tcggcggtga cgacttcgtc accaaccttg
      301 aggcgctgcg gggccaggat gtaagccagc tcgccatcgg tctgatactt gatcagggcg
      361 atgaacgcgg tgcggttggg gtcgtattca atacgaacaa ccgaagccac gccctgcttg
      421 cgacgcttga agtcgaccag gcgatagagg cgcttggcgc cgccgccgcg gaagcggaca
      481 gcgatacggc cattgccgcc gcgaccgccc gacttggtca ggccttcaac gagcttcttc
      541 tcgggacgac ccttgtagag ctcgctgcgg tcgatcagga ccaggccgcg ctggcctggg
      601 ctggccgggt taaagtgctt caaggccatc tgattagagc cccgtcgtga tgtcgatcga
      661 ctggccttcg gccagggtca cgatggcttt tttgacatcg gagcgacgtc cgatgatgcc
      721 gcggaagcgc ttggtcttgc ccttggtgac catggtgttg accttggtga ccttgacctt
      781 gaacagctct tcgactgcgg cggcgatttc gtccttggac gcatcggcgg acacccggaa
      841 gacaaccttg ttctgctcgg aaagcagggt ggccttttcg gtgatcaccg gcgacaggat
      901 cgtgtcgtag tggcgggcag ttgcggccat tacgcggctt ccttctcagc gaaacgggcc
      961 gagatcgcat ccaccgcgtc cttggtcagg accagggtgt ggcgacgcag cacgtcatag
     1021 acgttcaggc cggcgttcgg cagcacgtcg atgttcggaa tgttgcgagc ggcgagcttg
     1081 aagttgccgt ccacttccgg acccgcgatg accagggcgt tcttcagacc catcttttcg
     1141 aaggtggcgc gcagggcggc cgtcttcgga tcggtcagag caacgttgtc cagcacgatg
     1201 agcgagccgg ccttggcctt cgacgacaga gcgtgacgca gagccatcgc acggatcttc
     1261 ttgggaagat cgaacgcgtg gctacggacg acagggccgt gagccttggc gccgccgacg
     1321 aactgggccg cacggcgcga gccgtgacga gcgccgccgg tgcccttttg cttgtacatc
     1381 ttcttgcccg tgcgagagac ctcgttacgg acctgaatct tgtggttgcc ggagcggcgc
     1441 ttggccagct gccaggtgac gacgcgctgc aggatgtcgc cgcggatgtc ggcgatgcca
     1501 aagatggcgt cgtcgagatc aacggaaccg gccttgccgc cgtccagttt gatgacgtcg
     1561 agtttcatta gccttcgccc tcttcggtcg atgccgcggg ggcttcctca gcgggggttt
     1621 cagcagcggc ggtcgccgga gcctggccag ccttgcggaa ggcgcccgga cgcggcaggt
     1681 cggccggagc ggccttcttg atcgcgtcgc gaaccttcac gtagctgcct tcgtggccgg
     1741 ggacagcgcc cttgaccagg atcaggccgc gctcgacatc gactttcc
//
LOCUS       DAOMEO010000728         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6802_length_1787_cov_2.569284, whole genome shotgun sequence.
ACCESSION   DAOMEO010000728 DAOMEO010000000
VERSION     DAOMEO010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6802_length_1787_cov_2.569284"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /gene="ccmA"
                     /locus_tag="PLH31_14620"
     CDS             complement(<1..454)
                     /gene="ccmA"
                     /locus_tag="PLH31_14620"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HQR90463.1"
                     /translation="MLRTVLIQDLTISRGERRLFSDLSMALRAGEAVALTGRNGAGKT
                     SLLRAVAGLLRPEAGQIVFEDLEAETARAEHLHMVGHHDGLKSSRSAWEELRFQTLWS
                     GGSPATARQAAERLDLVRLLDLEVRRLSAGQRRRLALARLVASPRALWL"
     gene            636..>1787
                     /locus_tag="PLH31_14625"
     CDS             636..>1787
                     /locus_tag="PLH31_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQR90464.1"
                     /translation="MASVDSLKARRELIVGGKTYVYYSLRAAEEAGLTGVSSLPVSMK
                     VLLENLLRNEDGVSVDEPDLKAVAAWLQNKGAVEHEISFRPARVLMQDFTGVPAVVDL
                     AAMRDAMVALGADPAKINPLNPVDLVIDHSVMVDNFGNPKAYDDNVKREYERNIERYR
                     FLRWGSSAFNNFRVVPPGTGICHQVNLEYLAQTVWTNEVDGETTAYPDTVVGTDSHTT
                     MVNGLSVLGWGVGGIEAEAAMLGQPIPMLIPEVIGFKLNGAMPEGATATDLVLTVTQM
                     LRKKGVVGKFVEFYGDALATLTLEDQATIANMAPEYGATCGFFPISAATIAYLKGTGR
                     DAARVALVEAYAKEQGMWWEPGVPEPTFTDGLELDLSTVLPSLAGPKRPQ"
BASE COUNT          311 a          629 c          571 g          276 t
ORIGIN      
        1 gcagccacag ggcgcggggg ctggccacca gccgcgccag ggccaggcgc cggcgctggc
       61 cggccgacag acggcggacc tccaggtcga gcagacgcac caggtccagc cgctcggccg
      121 cctggcgcgc cgtcgcgggc gagccgccgc tccacagggt ctggaaccgc agttcctccc
      181 aggccgagcg gctggatttc aggccatcgt ggtggcccac catgtgcagg tgctcggccc
      241 gcgccgtctc ggcctccaga tcctcgaaaa cgatctggcc ggcttccggc cgcaaaagac
      301 ccgccacggc gcgcagcagg ctggtcttgc cggccccgtt tcgcccggtc agggccacgg
      361 cctcgccggc tcgcagcgcc atgctcaggt cagagaatag ccgccgctct ccccgggaga
      421 tcgtcaaatc ctgaatgaga acagttctca acatgccaag accctgcccc gcgcatggac
      481 gttggctgcg accaaggcta agaagccctc ggccgtcacc ctccaaacag tcaggggcgt
      541 acgcggcgcg gggatggacg ctgtgggtcc gcctcgatcg cgcgatcccg ggagtggttt
      601 tcgggcggcc gtggacagag aagggatctc ccgaaatggc gtctgtggac agccttaaag
      661 cccgccgcga actgatcgtc ggtggcaaga cctacgtcta ttacagcctt cgcgccgccg
      721 aggaagccgg tctgaccggt gtttcttcgc tgccggtgtc gatgaaggtg cttctcgaga
      781 acctgctgcg caacgaagac ggcgtgtccg ttgacgaacc cgacctgaag gccgtggccg
      841 cctggctgca gaacaagggc gccgtcgagc acgaaatcag cttccgcccc gcgcgcgtgc
      901 tgatgcagga cttcaccggc gttcccgctg tggtcgacct ggccgccatg cgcgacgcca
      961 tggtggccct gggcgccgac ccggccaaga tcaatccgct gaaccccgtt gacctggtca
     1021 tcgaccactc ggtgatggtc gacaatttcg gcaatcccaa agcctatgac gacaacgtca
     1081 agcgcgagta cgagcgcaac atcgagcgct atcgcttcct gcgctggggc tcgtcggcct
     1141 tcaacaactt ccgcgtcgtg ccccccggca ccggcatctg ccatcaggtg aacctggaat
     1201 atctggccca gaccgtctgg accaacgagg tcgacggcga aaccaccgcc tatcccgaca
     1261 ccgtcgtcgg caccgacagc cacaccacca tggtcaatgg cctgtcggtc ctgggctggg
     1321 gcgtgggcgg catcgaggct gaagccgcca tgctgggcca gccgatcccg atgctgatcc
     1381 ccgaagtcat cggcttcaag ctgaacggcg ccatgccgga aggcgcgacg gccaccgacc
     1441 tggtgctgac cgtcacccag atgctgcgca agaagggcgt ggtcggcaag ttcgtcgaat
     1501 tctacggcga cgccctggcc accctgaccc tggaagatca ggccaccatc gccaacatgg
     1561 ctccggaata tggcgcgacc tgcggcttct tcccgatctc ggccgcgacc atcgcctatc
     1621 tgaagggcac cggccgcgac gccgcccgcg tggcgctggt cgaagcctat gccaaggaac
     1681 agggcatgtg gtgggagccg ggcgtgcccg agcccacctt caccgacggc ctggaactgg
     1741 acctgtcgac ggttctgccg tcgctggctg gccccaagcg tccgcag
//
LOCUS       DAOMEO010000729         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6814_length_1784_cov_2.251591, whole genome shotgun sequence.
ACCESSION   DAOMEO010000729 DAOMEO010000000
VERSION     DAOMEO010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6814_length_1784_cov_2.251591"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..749)
                     /locus_tag="PLH31_14630"
     CDS             complement(351..749)
                     /locus_tag="PLH31_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90465.1"
                     /translation="MRVWFVRAAAAASLLAFGLTGCSDGGATTKVAQAQKVVLEAKSV
                     TASGCVRPVETTSCLVVRGRAGGYYDISSASPAPDPTKGVAISLQGKDLGKNTECGRQ
                     LTDVKWSYLGIQCAAPSTSASTDKADKKAG"
     gene            1021..>1784
                     /locus_tag="PLH31_14635"
     CDS             1021..>1784
                     /locus_tag="PLH31_14635"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQR90466.1"
                     /translation="MTAAPVPAAPAPIKEAAQSVETVLWVKHWTDRLFSFAITRPASF
                     RFRSGEFVMIGLPPREDLGEKKPILRAYSIGSPSFAEELEFFSIKVPDGPLTSRLQQI
                     QPGDQVLLGKKPTGTLVLDAVRPGKRLFLFGTGTGLAPWLSVARDPDAYSRFERVIVA
                     HGVREVQELAYRDFFTSEIFDDPLVGDEARAQLTYYPTVTREPFERQGRFTDLIQSGQ
                     LFRDLGLDSDRFDPEHDRAMLCGSMAMIKDTAALLEA"
BASE COUNT          319 a          631 c          525 g          309 t
ORIGIN      
        1 ggtcatggcc ttgttctgcg ccaggcggac gctgccgccg tgcgaaagcc ctgccgattg
       61 gctgcaggga gggatcaaag cgcggcgctt tcgcgttagg cagacatgac ccagtcagcc
      121 acgacccaac ccgtcaatga caacgccgcc tacgctgcgg cgcgcccgtc agatcgctct
      181 gccgagaacg agaaccttcg ccctccgtcg caggacggcg aggcgccccg ccccgcgaca
      241 gagccgcctg gcagcgaaaa cagcacccag tcacccaaga cccgcacgga tccagcatca
      301 ggcgagaact gaaccgtcgg gcggtcgctt gcgcgccgcc cgcagcggga tcagcctgcc
      361 ttcttgtcag ccttgtcggt cgaggccgag gtcgacggcg ctgcgcactg gatccccaga
      421 taggaccact ttacgtccgt cagctgacgg ccgcattcgg tgttctttcc gagatccttg
      481 ccttgcaaag agatcgccac acccttggtc gggtcgggcg ccggactggc ggagctgata
      541 tcgtaatatc cgcccgcacg accacgaacc accagacacg acgtggtctc gaccggcctt
      601 acgcagccgg acgccgtgac agacttggct tccaaaacaa ccttctgggc ctgcgcgact
      661 ttcgtcgtcg cgccgccatc gctgcaaccg gtcaaaccaa acgctaacag actggccgcc
      721 gcggcggccc tcacgaacca tacccgcata cgttcctccg tatcgttggc tccgcgaacg
      781 aagcaaagtg aagtttgcgc tccgtttgga tagccggcaa tcacattttt gacgcgtttc
      841 gaaaaagcgt tgccgcacat tttgacgatt tggcgaaaga ttttcccgga gcgccatcag
      901 aggagctgag ttgacttctc cgccccgctc ctcaaatgaa accagcgtcg cctcagggcc
      961 gtggcgacgc cactgaattg accttttcgc gcgtctgcgc ccaggaccga aacattgacg
     1021 atgacggccg ccccagtccc cgcagccccc gccccgatca aggaagccgc ccagtcggtc
     1081 gagaccgtgc tgtgggtgaa acactggacc gaccgcctgt tcagcttcgc catcacccgt
     1141 ccggccagct tccgcttccg ctcgggcgag ttcgtaatga tcggcctgcc gccccgcgaa
     1201 gacctgggcg agaaaaagcc gatcctgcgc gcctactcga tcggctcgcc cagctttgcc
     1261 gaggagctgg aattcttttc gatcaaggtc cccgacggcc ccctgacctc gcgcctgcag
     1321 cagatccagc ccggtgatca ggtcctgctg ggcaagaagc cgaccggcac cctggtgctg
     1381 gacgccgtcc gccccggcaa gcgcctgttc ctgtttggca ccggcacggg tctggcgccg
     1441 tggctgtcgg tggcccgcga tcccgacgcc tatagccgct tcgagcgcgt gatcgtcgcc
     1501 catggcgtgc gtgaagtgca ggagctggcc tatcgcgact tcttcacctc ggagatcttc
     1561 gacgatccgc tggtcggcga cgaggccagg gcccagctga cctattatcc caccgtgacc
     1621 cgcgagccgt tcgagcggca gggccgcttt accgacctga tccagagcgg ccagctgttc
     1681 cgcgatctgg gcctggacag cgaccgcttc gaccccgagc acgaccgcgc catgctgtgc
     1741 ggctcgatgg ccatgatcaa ggataccgcc gccctgctgg aggc
//
LOCUS       DAOMEO010000730         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6815_length_1784_cov_2.206478, whole genome shotgun sequence.
ACCESSION   DAOMEO010000730 DAOMEO010000000
VERSION     DAOMEO010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6815_length_1784_cov_2.206478"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..982
                     /gene="fdhD"
                     /locus_tag="PLH31_14640"
     CDS             167..982
                     /gene="fdhD"
                     /locus_tag="PLH31_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQR90467.1"
                     /translation="MPNPSVTRRSAEQWRAGAETQSIERAVPDEVAVGLSFDGRPHTV
                     LMATPADVEDLARGFTLTEAVAVAADITGIEVRETELGLLADVRLRAGTANRKARPRT
                     LEGRSSCGLCGVQRLSDAVRPLPKVGEGRRLSHQAVQRAVDALAGAQTLGRLTRATHA
                     AAFADAGGRILLSREDVGRHNALDKLAGAMAVAGEDATAGFILVTSRCSFEMVEKAVR
                     IGCPVLVAVSAPTDLAIRKAEEAGLTLIALARSDGHTVFTRADRMVEAPLETV"
     gene            982..>1784
                     /locus_tag="PLH31_14645"
     CDS             982..>1784
                     /locus_tag="PLH31_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR90468.1"
                     /translation="MSDAKIPGVRAYDAPAGGWGALKAVAGALGDQATIIEGGKTLLS
                     ANQPEGFDCPGCAWPDPKHTSSFEFCENGAKAVAWEATSKRATPEVFERHTVTELLTW
                     SDHAIEDLGRLTQPMAYDAAADRYRAISWDDAFARTGAALRALDHPDQAEFYASGRAS
                     NEAAFLYQLLGRRFGTNNFPDCSYMCHKPTSVGLPDSIGAGKGSVTLEDFDHADLILC
                     FGHNPGTNHPRMMGTLREASRRGAAILAFNPLRERSLEKFASPQDAVEMA"
BASE COUNT          262 a          612 c          630 g          280 t
ORIGIN      
        1 cagtcacctg ttgatctaag cgaaggcccc ggcggaaacg ccggggcctt ttgctgtcct
       61 gggctacccc acgaaggatg ctcggcttgc agggtgacgc ggtgcgccta tccggggttt
      121 cgcatttgtc gtgacgacgc gcgcctgcgc gcgctaggct gagcgcatgc cgaacccttc
      181 cgtcacccgc cgttccgccg agcagtggcg cgccggcgcc gagacccagt cgatagagcg
      241 ggccgtgcct gacgaggtcg ccgtgggcct gtcgttcgat ggtcggccgc acacggtgct
      301 gatggccacg cccgctgatg tcgaggatct ggcgcgcggc ttcaccctga ccgaggccgt
      361 ggccgtggcg gccgacatta caggtattga ggttcgcgag accgagctgg gcctgctggc
      421 cgatgtgcgc ctgcgggccg ggacggcgaa ccgcaaggcg cggccgcgta cgctggaagg
      481 acgctccagc tgtggcctgt gcggcgttca gcgcctgtcg gacgcggtgc gaccgctgcc
      541 gaaggtgggc gaggggcggc gtctttccca tcaggccgtt cagcgcgcgg tcgatgcgct
      601 ggccggggcc cagaccctgg gccgcctgac gcgcgccacc catgctgcgg ctttcgccga
      661 cgccgggggc cgcatcctgc tgtctcgcga ggacgtcggc cgccacaacg cgctcgacaa
      721 gctggccggg gccatggccg tcgcgggcga ggatgcgacg gccggcttta tactggtcac
      781 cagccgctgc tcgttcgaga tggtcgagaa agccgtccgc atcggctgcc ccgtactggt
      841 ggccgtttcg gcgccgacgg acctggccat ccgtaaggcg gaggaggcgg ggctgaccct
      901 gatcgccctg gcgcgctcgg acggccatac cgtcttcacc cgtgccgatc gcatggttga
      961 agccccgctg gagaccgtct gatgtcagac gcaaagattc cgggcgtccg cgcctatgat
     1021 gcgccagcgg gcggctgggg cgccctgaag gccgtcgccg gcgcactggg cgaccaagcc
     1081 acgatcatcg agggcggcaa gaccctgctg tcggccaacc agcccgaggg cttcgactgc
     1141 cccggctgcg cctggcccga tcccaagcac accagctcat tcgagttctg cgagaacggc
     1201 gccaaggcgg tggcctggga ggcgaccagc aagcgcgcta cgccagaggt gttcgagcgc
     1261 cacacagtga ccgagctgct cacctggagc gatcacgcca tcgaggatct gggccggctg
     1321 acccagccca tggcctatga cgccgccgcc gaccggtatc gggcgatctc gtgggacgac
     1381 gccttcgcgc ggacgggggc tgcgctgcgg gcgctggacc atcccgatca ggccgagttc
     1441 tatgcttcgg gccgggcctc caacgaggcg gcgtttctct atcagctgct gggccggcgg
     1501 ttcgggacca acaatttccc cgactgctcc tacatgtgcc acaaaccgac cagcgtcggc
     1561 ctgccggact cgatcggcgc gggcaagggc tcggtcacgc tggaggattt cgaccacgcc
     1621 gacctgatcc tgtgctttgg ccacaatccc ggcaccaacc acccccggat gatgggaacc
     1681 ctgcgcgagg cgtcaaggcg cggcgcggcg atcctggcgt tcaacccctt gcgcgagcgg
     1741 tcgctggaga agttcgcctc gccgcaggac gcggtggaga tggc
//
LOCUS       DAOMEO010000731         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6821_length_1783_cov_2.240741, whole genome shotgun sequence.
ACCESSION   DAOMEO010000731 DAOMEO010000000
VERSION     DAOMEO010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_1783_cov_2.240741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1423)
                     /gene="flgK"
                     /locus_tag="PLH31_14650"
     CDS             complement(2..1423)
                     /gene="flgK"
                     /locus_tag="PLH31_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HQR90469.1"
                     /translation="MSLNSIMSTASSGMMAAQTGLRVVSDNIANVNTAGYVRKTISQS
                     NLISNGMGVGVNIDAIKRATDRFLQSASLNAVSDTGRAAALSDALHTAQNLFGDPSGE
                     NSFFGRLDDVLSAFSAASDDPASSLLRTQALSRVDDFLGESSRITAALSNLGKDVDNR
                     IATDVERVNELLKQINALNTDITRAKVAGSDGTGSENVQGGLIDELSTLINIQVSQRA
                     NGGVIVRSTEGLNLAGDGAAVVSYQKSGTATGFLQVIQANGTDTPVSLNISSGEIKGL
                     LDLRNTELPALSDQLGEFVSRAAEELNRASNASSSVPAPATLTGRNTGLDEATAMSGF
                     TGKTTVALTDSAGVIQRQIEIDFDAGSMTVDGAAGPSFTNTDFIAQLNAALGGQGTAG
                     FSGGVLSLSANGGRGVVVADDATTPSNKIGKGFSHFFGLNDIVKSNGFSPYETGLTAT
                     DPHGFNPGDVTPISHPAITRVLG"
BASE COUNT          333 a          524 c          603 g          323 t
ORIGIN      
        1 atcaacccag gactctcgtt atcgctggat gagaaatggg ggtcacgtca cccggattga
       61 agccgtgcgg atcagtggcc gtaaggcccg tctcgtacgg cgagaagccg ttcgatttga
      121 cgatgtcgtt gaggccgaag aagtggctga agcccttgcc gatcttgttg gacggtgtgg
      181 tcgcatcatc agccaccacc acgccgcgcc ccccgttggc gctaaggctc agcacgccgc
      241 cgctgaaccc ggcggtgccc tggccgccga gcgccgcgtt cagttgggcg atgaagtcgg
      301 tattggtgaa gctgggtccc gcggcgccgt cgacggtcat cgagccagcg tcgaaatcga
      361 tctcgatctg tcgctggatg acgcccgcgg agtcggtgag ggcgacggtg gtcttgccgg
      421 tgaagccgct catggcggtg gcttcatcga ggccggtatt gcgaccggtc agggtggctg
      481 gagcggggac cgaggaagag gcgttggaag ctcgattcag ctcctccgcc gcgcgcgaga
      541 caaattcgcc cagctgatcg gacagggctg gcagttcggt gttgcgcagg tccagcaggc
      601 ccttgatctc accgctcgag atattcagcg agaccggcgt atcagtgccg ttggcctgga
      661 tcacctgcag gaagccggtg gcggtaccag acttctggta cgagacgacc gccgcgccgt
      721 cgccggccag attcaggccc tcggtcgagc gcacgatgac gccaccgttc gcgcgttggc
      781 tgacctggat gtttatcagg gtcgacaact cgtcaatcag gccgccctgc acgttctccg
      841 aacctgtgcc atcagaaccg gccaccttgg cgcgggtgat gtcggtgttg agcgcgttga
      901 tctgcttgag cagttcgttg acccgctcca catccgtggc gatgcggttg tcgacgtcct
      961 tgcccagatt ggagagggcg gccgtgatcc ggctggattc gcccagaaag tcatccacgc
     1021 gcgagagggc ctgggtgcgc aacaggctgg aagccgggtc atcggaagcg gccgagaacg
     1081 cagacagcac atcatccagc cggccaaaga agctgttctc gccgctgggg tcgccaaaca
     1141 ggttctgggc ggtgtgcagg gcgtcggaca gggccgccgc gcggccggta tcggagaccg
     1201 cattcaggct ggccgattgc aggaagcgat cggtggcgcg cttgatggcg tcgatattga
     1261 cgccgacgcc catgccgttg gagatcaggt tcgactggct gatcgtcttg cggacatagc
     1321 cagcggtgtt gacgttggcg atgttgtccg agacgacgcg caggcccgtc tgggccgcca
     1381 tcatccccga actcgccgtg ctcatgatgg aattcaaaga catgcggccg ctcccgtcag
     1441 ctcggcaatc cgcgaaccac gcgacgcgcc cttgcccggc gaaaactgcc gggcggggga
     1501 ggggccaatg ccctcgatat atgccaagcc actgaaattc atggcgtatt gttccaagtg
     1561 ccgggtattc ggcaggatgt gatgcgcaag gagggcgcca agtgggggcg cgaccgggcg
     1621 acgcggcaag ccacgcccct gacaaccccg ttcgactcgg caaaaagcgc cgcagaccga
     1681 cgatctcgag ttataaacaa gacattaaag ttcatgaaaa caatgggatg gcggaatatt
     1741 tcgagttggc ccgacggttg ctgaaggagg ttcggacaaa ggc
//
LOCUS       DAOMEO010000732         1782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6823_length_1782_cov_2.568616, whole genome shotgun sequence.
ACCESSION   DAOMEO010000732 DAOMEO010000000
VERSION     DAOMEO010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6823_length_1782_cov_2.568616"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..653)
                     /gene="fixJ"
                     /locus_tag="PLH31_14655"
     CDS             complement(51..653)
                     /gene="fixJ"
                     /locus_tag="PLH31_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HQR90470.1"
                     /translation="MTEAVVHVVDDDESARESLAFLLESADFQVVSYESGPAFLDALP
                     TARPGCVITDVRMPEMSGQELVARLAALNARMPVIMITGHGDIPMAVEAMRSGVVDFI
                     EKPFSEGRILDALRRALNALEAPSMSDDQAEIQHRLETLSERERQVLDGVVAGHPNKV
                     IARELGISPRTVEIYRAKLMTKMHADNLAALVRMTLSVRG"
     gene            complement(646..>1782)
                     /locus_tag="PLH31_14660"
     CDS             complement(646..>1782)
                     /locus_tag="PLH31_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR90471.1"
                     /translation="HAARRRAAATNAHLQSILDTVPDAVVVIDRDGVMTSFSPAAERL
                     FGWAAAEAIGQNVTLLMPSPYRQAHDGFLRRYAQTGEKRIIGIGRVVVGERKDGSTFP
                     MELAVGETRGPRPSYTGFIRDLTDRQQTETRLQELQNELVHVSRLTAVGEMASALAHE
                     LNQPLSAIANLLTGSRRLIDRGRPADQAKVRDAIDRAAAQALRAGDVIHKMRDFVRRG
                     ASEREVESLSKLVEEASALALIGEKERQVDVRLSLDPQADQVFADRVQIQQVLLNLIR
                     NGIDAMENLPRRELVISSDLNEEGWTRVSVADSGSGITDAVREKLFQPFMTTKPQGMG
                     VGLSISRSIIEAHGGRIWAEVKTGGGTIFRFTLPPADQEDDIDD"
BASE COUNT          298 a          587 c          580 g          317 t
ORIGIN      
        1 ttagagcatt tttcgatccg ataaccgttt cacacttatc ggaaaatgct ctagccgcgc
       61 accgacaggg tcatccgcac cagggcggcc agattgtcgg catgcatctt ggtcatcagc
      121 ttggcgcgat agatctcgac cgtccggggg ctgataccca gctcgcgggc gatcaccttg
      181 ttgggatggc cggcgaccac gccgtccagc acctggcgct cgcgttcgga cagcgtctcc
      241 aggcgatgct ggatctccgc ctgatcatcg gacatcgagg gcgcttccag agcgttcagg
      301 gctcgcctga gagcatccag aatacggcct tccgagaagg gcttttcgat gaagtccacg
      361 acgcctgacc gcatggcttc gaccgccatc gggatatcgc catggccggt gatcatgatg
      421 acgggcatgc gggcgttcag cgcggccagt cgcgcgacca gctcctgacc gctcatctcg
      481 ggcatgcgga catcggtgat cacgcagcca ggtcttgcgg tcggcagggc gtccaggaag
      541 gcgggtccgc tctcatagct gacgacctgg aaatcggccg actccagcag gaaggccagg
      601 gactcccggg cgctttcatc gtcgtcgacc acatgaacca cggcctcagt catcaatatc
      661 gtcctcctgg tcggcgggcg gcagggtgaa acggaagatc gtcccgcccc ccgttttgac
      721 ttcggcccag atccgtccgc catgcgcctc gatgatcgag cgcgagatcg aaagccccac
      781 gcccataccc tgcggcttgg tggtcatgaa gggctggaac agtttttcac gcaccgcatc
      841 ggtgatgccg cttccactgt cggcgacgct gacccgggtc cagccctcct cgttcaggtc
      901 gctggaaata acaagttcgc gccgcggcag gttttccatg gcgtcaatgc cgttgcggat
      961 caggttcagc agcacctgct ggatctggac gcggtcggcg aagacctgat cggcctgggg
     1021 atccagcgac agtcgcacat cgacctggcg ttccttctcg ccgatcaggg ccagggcgct
     1081 ggcctcctcg accagcttgg acaggctctc gacttcgcgc tcgctggcgc cgcgccgaac
     1141 gaagtcgcgc atcttgtgga tcacgtcacc ggcccgcagc gcctgggcgg cggcgcggtc
     1201 gatcgcgtcg cgcaccttgg cctggtcggc cgggcgaccc cggtcgatca atcggcgcga
     1261 cccggtcaac aggttggcga tggccgagag cggctgattc agctcgtggg ccagggccga
     1321 ggccatctcg ccaacagcgg tcaggcgcga cacatggacc agttcgttct gcagctcctg
     1381 caggcgggtc tcggtctgct ggcggtcggt caggtcgcga atgaagccgg tataggaggg
     1441 gcgtgggccg cgggtctcgc cgaccgccag ctccatggga aaggtcgagc cgtccttgcg
     1501 ttcgccgacc accacgcggc cgataccgat gatccgcttc tcacccgtct gggcgtagcg
     1561 gcgcagaaag ccgtcatggg cctgacgata gggactgggc atcagcaggg tgacattctg
     1621 accaatagcc tcggccgccg cccagccaaa caggcgctcg gccgcggggc tgaacgaggt
     1681 catcaccccg tcgcggtcga tgaccaccac ggcgtctggc acggtgtcga ggatcgactg
     1741 caggtgggcg ttggtcgccg ccgcccgccg ccgcgcggca tg
//
LOCUS       DAOMEO010000733         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6826_length_1781_cov_1.881228, whole genome shotgun sequence.
ACCESSION   DAOMEO010000733 DAOMEO010000000
VERSION     DAOMEO010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_1781_cov_1.881228"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..>1781
                     /locus_tag="PLH31_14665"
     CDS             124..>1781
                     /locus_tag="PLH31_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQR90472.1"
                     /translation="MNDWTLPPYRFEDGPKPPQRPEVSFGASPPPPPPPPPEPPPEPE
                     EPFRADLKHQKARKKANRFGWIWKTLLVGVILGVLTVGAGAGYVWWKYLRDTPKLPSR
                     EALFAVNRAPGIRFEDKNGQVIATRGPRYGQRVTLDGLPNYVPLAFMAAEDRRFYKHG
                     PIDLRGIGRAAIVNWKAGRTVQGASTLSQQLAKGLFLTPDRTVKRKLQEMVMANELEK
                     VLTKDEVLELYLNRVFFGANTFGVDGASRTYFGKPASQLTLSEAALLASLPKAPSRLA
                     LTRDMSGALARSRLVLANMRKEGWITAEQESRALDDTPRLSPLAVQDEGIYGWVLDYA
                     TNEAVRIAGQNAPDLVVRLTIDPLLQKLGSETVRQAIAFDGERAGAGQAALLSLSADG
                     AIRAMVGGTDYIESPFNRAVQARRQPGSTFKPFIYAAALDKGVLPTDIRVDAPVKFGD
                     WEPENYAGGYRGPMTVEQALVNSTNTIAVKLGQEAGGPAIGELVRRFGITTLPPSPDL
                     SVALGSYEVNLLQLTSGFQVFQQGGNRIEPYVIESIVTQGGAPI"
BASE COUNT          306 a          638 c          564 g          273 t
ORIGIN      
        1 actctcccac tcggggacgg tcatctgctg gagggacttc gtctcccaga aaggtttgcg
       61 caccatcatg cgggcgtcct aaaccgaaac taggctaaga ccaaacaggg ccgtatcgaa
      121 accgtgaacg actggacgct tcccccgtac cgtttcgaag atggccccaa gccgccgcaa
      181 aggccggagg tttcctttgg cgcgtcgccg ccgcccccgc cccccccgcc tccagagccg
      241 ccgccagaac ctgaagagcc gttccgcgcc gacctgaagc accagaaggc tcgcaagaag
      301 gccaaccgct ttggctggat ctggaagacc ctgctggtcg gcgtgatcct gggcgtcctc
      361 accgtgggcg ccggggccgg ctatgtctgg tggaaatacc tgcgcgacac gccaaaactg
      421 cccagccgcg aggccctgtt cgccgtcaac cgcgccccgg gcatccggtt tgaggacaag
      481 aacggccagg tgatcgccac ccgcggcccg cgctatggcc agcgcgtcac cctggacggc
      541 ctgcccaact atgtgcccct ggccttcatg gccgccgagg accgccggtt ctacaagcac
      601 ggcccgatcg acctgcgcgg catcggccgg gcggcgatcg tcaactggaa ggccggccgc
      661 accgtccagg gcgcctccac cctcagccag caactggcca agggcctgtt cctgacgcct
      721 gaccgcacgg tcaagcgcaa gctgcaggaa atggtcatgg ccaatgagct ggaaaaggtg
      781 ctgaccaagg acgaggtgct ggagctgtac ctcaaccggg tgttcttcgg agccaacacc
      841 ttcggcgtgg atggcgcgtc acggacctat ttcggcaagc ccgccagcca gctgaccctg
      901 tcggaagccg ccctgctcgc ctcgctgccc aaggcgccgt cgcgcctggc cctgacccgc
      961 gacatgagcg gcgccctggc ccgctcgcgc ctggttctgg ccaatatgcg caaggaaggc
     1021 tggatcaccg ccgagcagga gagccgggct ctcgacgaca ccccgcgcct gtcgccgctg
     1081 gccgtgcagg acgagggcat ttacggctgg gtgctggact acgccaccaa cgaggccgtg
     1141 cgcatcgccg gccagaacgc ccctgatctg gtggtgcgcc tgaccatcga ccccctgctg
     1201 cagaagctgg ggtcggaaac cgtgcgccag gccatcgcct ttgacggcga gcgggccggc
     1261 gccggccagg cggccctgct gtcgctgtcg gccgacggcg ccatccgggc catggtgggc
     1321 ggcaccgact atatcgaaag cccgttcaac cgggccgtcc aggcccgtcg ccagccgggc
     1381 tcgaccttca agccgttcat ctatgccgcc gccctcgaca agggggttct gcccaccgac
     1441 atccgcgtcg atgcgcccgt gaagttcggc gactgggagc cggaaaacta tgccggcggc
     1501 tatcgcggtc cgatgaccgt ggaacaggcc ctggtcaatt ccaccaacac catcgccgtg
     1561 aaactgggcc aggaagccgg cggtccggcg atcggcgaac tggtccggcg tttcgggatc
     1621 accaccctgc cgcccagccc cgacctgtcg gtggcgctgg gctcgtatga ggtgaacctt
     1681 ctgcagctga cctcgggctt ccaggtgttc cagcagggcg gcaatcgcat cgagccctat
     1741 gtgatcgaga gcatcgtcac ccagggcggc gccccgatct t
//
LOCUS       DAOMEO010000734         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6829_length_1780_cov_2.551884, whole genome shotgun sequence.
ACCESSION   DAOMEO010000734 DAOMEO010000000
VERSION     DAOMEO010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_1780_cov_2.551884"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>438)
                     /locus_tag="PLH31_14670"
     CDS             complement(176..>438)
                     /locus_tag="PLH31_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQR90473.1"
                     /translation="GEDAADLLERHGFEALALSPAAPEILAHLKPPERSAVLLGAEGP
                     GLSDALMARARPIGIPMAGGFDSLNVATTSGVVLHHLRFADR"
     assembly_gap    439..452
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<453..911)
                     /locus_tag="PLH31_14675"
     CDS             complement(<453..911)
                     /locus_tag="PLH31_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR90474.1"
                     /translation="MPQFITVTDPDDPRVAAYRDIRERDLVGRQGLFIAEGETVLRAF
                     VRDAPQRVMSLLIDPKRRDKLDEVFAGLPDEAPVYLVEQAVLDAVAGFHLHRGVLAVG
                     RKAEPVPAETLLASLKGRAVVLVLCGIANHDNIGGIFRNAAAFGADAILLD"
     gene            complement(932..>1780)
                     /locus_tag="PLH31_14680"
     CDS             complement(932..>1780)
                     /locus_tag="PLH31_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90475.1"
                     /translation="LPDRVPDAQKGRVAAFLSLGNPIGTMAGAVLIGGLLVTEFTRYI
                     AISLVLLLTLAPFVLKLDDKPLPRDARPPFKLGAFIAALWVNPRKHPDFAFAWVGRFL
                     VLVALSLVQCYMLYFLQDVIGYSRIFPGRKAEEGLAILTTVSTVSNVALALIGGMLSD
                     KLKRRKLFAAGAAMTIALAMVIFSMTPSWPVMVATYLLLGCGAGCFYAVDIALVTQVL
                     PSQRDAGKDLGVLNLANTLPQALAPMLAVWALGPTQLDYQVFFLVAATLAVAGGLAIL
                     PIRGVR"
BASE COUNT          339 a          589 c          605 g          233 t
ORIGIN      
        1 ggatcgggca tgggcagggt ctccgctttc gccccgcgcg ccaggctcga ggcccaaagg
       61 ccagtgacga aggccgccag agcgccgcga cgattgagca tggacgaacc ttccggcgaa
      121 ctgactgaac tgccgccgac cttaggaccc gccccggatt caggaaaccg gtttcctagc
      181 ggtctgcgaa gcgcagatga tgcaggacga cgccgctggt cgtggccacg ttcagggaat
      241 cgaagccgcc cgccatcggg ataccgatag gccgggcgcg cgccatcagg gcgtccgaca
      301 gcccgggtcc ttccgcgccc agcagtacgg ccgagcgctc gggcggcttc aggtgagcca
      361 ggatttcggg ggcggccggc gacagggcca gggcctcgaa gccgtggcgc tccagcaggt
      421 cggcggcgtc ctcgccacnn nnnnnnnnnn nnatccagca ggatggcatc ggcgccgaag
      481 gcggcggcgt tgcggaagat gccgccgata ttgtcgtgat tggcgatgcc gcacagcacc
      541 agcaccaccg ccctgccctt cagcgacgcc agcaacgtct cggccggcac gggttcggcc
      601 ttgcggccca cggccagcac cccgcgatgc agatggaagc cggcgacggc gtcgagcacc
      661 gcctgctcga ccagatagac cggcgcctcg tccggcagtc cggcgaacac ctcgtccaac
      721 ttgtcacggc gcttgggatc gatcagcagc gacatcaccc gctggggcgc gtcgcggacg
      781 aaggcccgca gcaccgtctc gccctcggcg atgaacaggc cctggcgtcc gaccagatcg
      841 cgctcgcgga tgtcgcgata ggccgcgacg cggggatcgt ccggatcggt gacggtgatg
      901 aactgcggca cggcggcgtc ttccgaaagg cttaccgcac gcctcgtatc ggcaggatgg
      961 ccagaccgcc agccacggcg agcgtggcgg ccaccaggaa gaagacctga tagtccagct
     1021 gggtgggacc cagcgcccag acggccagca tcggcgccag ggcctggggc aaggtgttgg
     1081 ccaggttcag cacgcccaga tccttgcccg catcgcgctg ggacggcagc acctgcgtga
     1141 ccagggcgat atcgactgca tagaaacagc ccgccccgca gcccaggagc agataggtcg
     1201 ccaccatcac cggccagctg ggcgtcatcg aaaagatcac catggccaga gcgatggtca
     1261 tggcggcgcc tgctgcgaac agcttgcgac gcttgagctt atcggagagc atcccgccaa
     1321 tcagggccag ggcgacattg gacaccgtgg agacggtggt caggatcgcc agcccctctt
     1381 cggccttgcg acccggaaag atcctggaat agccgatcac gtcctgcagg aaatacagca
     1441 tgtagcactg cacgaggctg agggcgacga gcaccaggaa gcgtccaacc caggcaaagg
     1501 cgaagtcggg gtgtttgcgc ggattcaccc agagcgcggc gatgaaagcg cccagcttga
     1561 acggcggccg ggcgtcgcgc ggcagcggct tgtcgtcaag cttcagcaca aagggggcca
     1621 gggtcagcag caacaccagc gagatcgcaa tatagcgggt gaactcggtc accagcaggc
     1681 caccgatcaa cacggcgccg gccatcgtgc caatcgggtt gcccaggctg aggaaggcgg
     1741 ccacgcggcc cttctgggcg tcgggcacac ggtcgggcaa
//
LOCUS       DAOMEO010000735         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6830_length_1780_cov_2.510725, whole genome shotgun sequence.
ACCESSION   DAOMEO010000735 DAOMEO010000000
VERSION     DAOMEO010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_1780_cov_2.510725"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>685
                     /locus_tag="PLH31_14685"
     CDS             3..>685
                     /locus_tag="PLH31_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQR90476.1"
                     /translation="MRSVVESAIQRASSATGVDFTFLMGTAKRESGYNPTAKARTSSA
                     SGLFQFVDQTWLGTLKKHGAKYGYARYADLISQGSDGRFRVAGDEARKAVLALKMDPH
                     AASLMAGELASDHASYLRGRVGRTPTAGELYAAHFLGPQGSARLIEAANKTPGASAAS
                     LFPEAAQANRSIFYREGRAATVSEVYANLTKTGGAGRATGAPRDSGADQGFVQYASGR
                     RLERLQQQEA"
     assembly_gap    686..695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <696..843
                     /locus_tag="PLH31_14690"
     CDS             <696..843
                     /locus_tag="PLH31_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90477.1"
                     /translation="ILRGSQDADTTFGSLGSGVGSGQRLASSMFSSEMLRVLAEAKDG
                     KLNR"
     gene            complement(833..>1780)
                     /locus_tag="PLH31_14695"
     CDS             complement(833..>1780)
                     /locus_tag="PLH31_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQR90478.1"
                     /translation="MKALVATADVFLTNVRPGALARAGLDHEALKAVNPRLIYCSLTG
                     YGLEGPDADKAGMDVAAFWSRAGVGSITAPKGAEPFPIRTGMGDHVTSLATVSAILAA
                     VFERQTTGVGRLVETSLLRAGVYAIGSDMAIQLRFGKLASTRGRREAIQPLANFFKTR
                     DGRWICLLPRQGATDWARIAEAAGRPELVDDPRFASAKARRENTAILVDLLDAAFGAM
                     DYEDVAAALDEGDLTWAPYQTPRELVGDPQAEAAGCFVDTPDGAGGTFKAPASPARFP
                     GALDGPRGPAPKLGADTAAVFKELGWTEDRIAALTGPSG"
BASE COUNT          269 a          622 c          618 g          261 t
ORIGIN      
        1 ccatccgcag cgtcgtagaa tcggcgatcc agcgcgcttc cagcgcgacc ggggtagatt
       61 tcacgttcct gatgggcaca gccaagcgcg agagcggcta caaccccacc gccaaggcgc
      121 ggacctcgtc ggcctccggt ctgttccagt tcgttgacca gacctggctg ggcacgctga
      181 agaagcacgg cgcgaagtac ggctatgccc gttatgccga tctgatctcg cagggctcgg
      241 acggtcgttt tcgggtcgcc ggcgacgagg cccgcaaggc cgtgctggcg ctgaagatgg
      301 acccgcacgc cgcctcgctg atggccggcg agctggcgtc ggaccacgcc tcctatctgc
      361 gcggccgggt cgggcggacg ccgacggcgg gcgagctcta cgcggcgcac tttctggggc
      421 cgcaaggctc ggcgcggctg atcgaggccg ccaacaagac ccccggcgcc agcgccgcgt
      481 cgctgtttcc cgaagccgcc caggccaatc gctcaatctt ctatcgcgaa ggtcgcgcgg
      541 ccaccgtcag cgaggtctat gccaacctca ccaagaccgg cggcgcgggt cgcgccacgg
      601 gcgcgccccg cgatagcggc gccgatcagg gctttgtcca atacgccagc gggcgccggc
      661 tggagcgcct gcagcagcag gaggcnnnnn nnnnngatcc tgcgcggctc tcaggacgcc
      721 gacaccacgt ttggcagcct gggttcgggc gtcggctcgg gccagcgtct ggccagctcc
      781 atgttctcca gcgagatgct gcgtgtcctg gccgaggcca aggacggcaa gctcaaccgc
      841 tagggccggt cagggccgcg atgcggtcct cggtccatcc cagttccttg aacaccgccg
      901 ccgtatccgc gcccagcttc ggcgcggggc cacgcggacc atccagcgcg cccggaaagc
      961 gggccggaga ggcgggggcc ttgaaggttc cgccggcgcc atcaggcgta tccacgaagc
     1021 agccagccgc ctcggcctgg ggatcgccaa ccagctcgcg cggcgtctgg tagggggccc
     1081 acgtcaggtc gccctcgtcg agggcggcgg ccacgtcctc gtagtccatg gcgccaaagg
     1141 ctgcgtccag caggtcgacc aggatggcgg tgttttcgcg ccgcgccttg gccgaggcga
     1201 agcgcgggtc gtcgaccagt tcgggcctgc cggccgcttc cgcgatccgg gcccaatccg
     1261 tcgcgccttg tcggggcaac aggcagatcc agcgcccgtc gcgggtcttg aagaagttgg
     1321 cgagcggctg gatcgcctcg cgccgtccgc gcgtcgaggc cagtttcccg aatcgcagct
     1381 ggatcgccat gtccgagccg atggcgtaga cgccggcgcg cagcagcgag gtctcgacca
     1441 agcggcccac gcctgtggtc tggcgctcga atacggccgc caggatcgcc gacaccgtgg
     1501 ccagagaggt cacgtggtcg cccatgccgg tgcggatcgg aaagggctct gcgcccttcg
     1561 gggcggtgat cgatccgacg ccagcccgtg accagaaggc ggcgacatcc atgccggcct
     1621 tgtccgcgtc cggaccctca agaccatagc ctgtgaggct gcaatagatc aggcggggat
     1681 tgaccgcctt cagcgcttcg tgatccaggc ccgcccgcgc cagggcgccg gggcggacat
     1741 tggtcaggaa cacgtcggcg gtcgcgacca gggccttcat
//
LOCUS       DAOMEO010000736         1777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6845_length_1777_cov_3.407085, whole genome shotgun sequence.
ACCESSION   DAOMEO010000736 DAOMEO010000000
VERSION     DAOMEO010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6845_length_1777_cov_3.407085"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1777)
                     /locus_tag="PLH31_14700"
     CDS             complement(<1..>1777)
                     /locus_tag="PLH31_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR90479.1"
                     /translation="DDEERPIGLVERNAFFLRMAAEYGRALYANRPISALMDVEPLVV
                     DADTALADFTTDSLSYRASDLLRGFIVTDHGRYLGVGTVLALLQAANESNRRGVAELA
                     AAKADAERAQTFMTAVVEAMPSMVFVKRADDHRYVLLNRAGEKTMGFSRAEMIGRTDA
                     DFFSPEQAAIYFDRDREVVESGEVRVIEEDLVPRKDGSLAILRTKKIAINDAEGRPEY
                     LLGVSEDIADRKRAEAQIARLAHYDPLTELPNRVLFQKDLVEALARRSRTGDRLAVHF
                     IDLDRFKTVNDTLGHPLGDALLRIAAERLRGCVREGDTVARLGGDEFAVVQTGLTDLS
                     GATKLAERVVEAMAAPFDLQGHQVMIGASVGVSVAPSDGDDADELLKKADMALYRAKA
                     DGRGAFHFFERAMDEQLQARRALELDLRRALAAGEFQLYYQPLYHLGDDRVTGCEALL
                     RWNHPDRGMVSPADFIPIAEEIGLIVQLGEWVLREACAEAANWPDHVRLAVNLSPAQF
                     RDRGLVRTVVSALAVSGLPAQRLELEITESVLLQDNVANMGMLHDLKALGVRISMDDF
                     GTGYSSLSYLRSFPFDKIKIDRSFVR"
BASE COUNT          294 a          577 c          624 g          282 t
ORIGIN      
        1 gcgcacgaag gagcgatcga tcttgatctt gtcgaacggg aagctgcgca gatagctcag
       61 cgagctgtag cccgtgccga agtcgtccat cgagatgcgc acgcccaggg ccttcagatc
      121 gtgcagcatg cccatattgg cgacattgtc ctgcagcagc accgactcgg tgatctccag
      181 ctccagccgc tgcgccggca ggcctgaaac cgccagggcc gagaccacgg tgcgcaccag
      241 gccccggtcg cggaactggg cgggcgacag attgaccgcc agacgcacgt gatcgggcca
      301 gttggccgcc tcggcgcagg cttcgcgcag cacccactcg cccagctgga cgatcaggcc
      361 gatctcctcg gcgatcggga taaagtcggc cggcgagacc ataccgcggt cgggatggtt
      421 ccagcgcaac agcgcctcgc aaccagtcac ccggtcatcg cccagatgat acagtggctg
      481 gtagtagagc tggaattcgc ctgcggcgag cgcgcggcgc aggtccagct caagggcccg
      541 gcgcgcctga agctgctcgt ccatggcccg ttcgaagaag tggaaggccc cgcgtccatc
      601 ggccttggcg cgatagagcg ccatgtcggc cttcttgagc aattcgtcgg cgtcgtcgcc
      661 gtcggagggc gcgacggaca cgccgacgct ggcgccgatc atcacctgat gaccctgcag
      721 gtcgaacggc gcggccatgg cctcgaccac gcgctcggcc agcttggtgg cgccggacag
      781 gtcggtgagg ccggtctgga ccaccgcgaa ttcgtcgccg cccaggcggg ccacggtgtc
      841 gccttcgcgc acgcagcccc gcaggcgctc ggcggcgatg cgcagcaggg cgtcgcccag
      901 cggatggccc agggtgtcgt tgacggtctt gaagcggtcc aggtcgatga agtgcacggc
      961 cagccggtcg cccgtgcgtg agcggcgggc cagggcctcg accaggtcct tctggaacag
     1021 cacgcggttg ggcagttcgg tcaggggatc atagtgggcc aggcgggcga tctgggcctc
     1081 ggcgcgcttg cgatcggcga tgtcttccga cacgcccagc aggtattcgg gccggccctc
     1141 ggcgtcattg atggcgatct tcttggtgcg caggatggcc aggctgccgt ccttgcgggg
     1201 caccaggtct tcctcgatca cccgcacttc gccggactcg acgacttccc ggtcgcgatc
     1261 gaaatagatg gccgcctgct ctggcgagaa gaagtcggcg tcggtgcggc cgatcatttc
     1321 ggcgcgggaa aagcccatcg tcttctcgcc ggcccggttc agcagcacat agcgatgatc
     1381 gtcggcgcgc ttgacgaaga ccatcgaggg catggcctcg accacggcgg tcatgaaggt
     1441 ctgggcgcgt tcggcgtcgg ccttggcggc cgccagttcg gccacgccgc ggcggttgct
     1501 ctcattggcg gcctgtagca gggccagaac cgtgccaacg cccagatagc gaccgtgatc
     1561 ggtaacgatg aagccacgca gcaggtcgga ggcgcgatag gacaggctgt cggtggtgaa
     1621 gtcggccagg gcggtgtcgg cgtcgacgac caggggctcg acatccatca gggccgagat
     1681 gggccggttg gcatagaggg cgcgaccata ttcggcggcc atgcgcagga agaaggcgtt
     1741 acgctcgacc agaccgatcg gccgctcctc gtcgtcg
//
LOCUS       DAOMEO010000737         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6880_length_1771_cov_2.817016, whole genome shotgun sequence.
ACCESSION   DAOMEO010000737 DAOMEO010000000
VERSION     DAOMEO010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6880_length_1771_cov_2.817016"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1771)
                     /gene="uvrB"
                     /locus_tag="PLH31_14705"
     CDS             complement(<1..>1771)
                     /gene="uvrB"
                     /locus_tag="PLH31_14705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQR90480.1"
                     /translation="DSGVSDVSTPFVMDVSSDPAMIARQTLWTPHRPERPAKSEGGKR
                     FKLVSEYSPAGDQPTAIAELVEGLENGDQDQVLLGVTGSGKTFTMAQVIEKTQRPALI
                     LAPNKTLAAQLYSEMKSFFPENAVEYFVSYYDYYQPEAYVPRTDTYIEKDSSINEQID
                     RMRHSATRAILERDDVIIVASVSCIYGIGSVETYTAMTFTLKVGQTVNEKQLMADLVA
                     QQYKRNDAAFERGTFRRRGDTLEIFPAHYEDRAWRISLFGDEVEAIAEFDPLTGKKTA
                     DLPSIKVYANSHHVTPRPTLRQAIIEIRAELKERLAWMYENGKLLEAQRLEQRTNFDL
                     EMIETTGSCAGIENYSRYLSGRKPGEPPPTFFEYIPDNALLFTDESHQTVPQIGAMYK
                     GDRSRKWTLAEYGFRLPSALDNRPLKFEEWDAMRPQSVHVSATPAAWELERAGGVFAE
                     QVIRPTGLIDPPVEVRPVSASGASQVDDVIDEIRQAKAKGYRTLVTVLTKKMAEDLTE
                     YINEQGLGVRYMHSDVDTLERIEIIRDLRLGKFDVLVGINLLREGLDIPEVSLVAILD
                     ADKEGFLRSERALIQTIGRAARHL"
BASE COUNT          248 a          529 c          648 g          346 t
ORIGIN      
        1 agatggcgcg cggcgcgccc gatggtctgg atcagcgcgc gctccgagcg caggaagcct
       61 tccttgtcgg cgtcgaggat cgccaccagc gacacttccg gaatgtcgag gccctcgcgc
      121 agcaggttga tgccgaccag cacgtcgaac ttgcccagcc gcaggtcgcg gatgatctcg
      181 atgcgctcca gggtgtcgac gtcggagtgc atgtagcgca cgcccaggcc ctgctcgttg
      241 atatattcgg tcaggtcctc ggccatcttc ttggtcagga cggtgaccag ggtgcggtag
      301 cccttggcct tggcctggcg gatctcgtcg atgacgtcat cgacctggct ggcgccgctg
      361 gcgctgaccg ggcggacctc gaccggcggg tcgatcaggc cggtggggcg gatgacctgc
      421 tcggcgaaga cgccgccggc gcgctccagc tcccaggcgg cgggggtggc gctgacatgc
      481 accgactggg gccgcatggc gtcccattcc tcgaacttca gcgggcggtt atccagcgcg
      541 ctgggcaggc ggaagccgta ctcggcaagc gtccatttgc ggctgcggtc gcccttgtac
      601 atggcgccga tctgcggaac cgtctggtgg ctctcgtcgg tgaacagcag ggcgttgtcg
      661 gggatatatt cgaagaaggt cggcggcggc tcgccgggtt tgcggccgga gagatagcgg
      721 ctgtagtttt cgatgccggc gcaggagccg gtggtctcga tcatctccag gtcgaaattg
      781 gtccgctgct ccaggcgctg ggcctccagc agcttgccgt tctcgtacat ccaggccagg
      841 cgctccttga gctcggcgcg gatctcgatg atcgcctggc gcagggtggg gcgcggcgtg
      901 acgtggtggc tgttggcgta gaccttgatg ctgggcaggt cggcggtctt cttgccggtc
      961 agcgggtcga actcggcgat ggcctcgacc tcgtcgccga acaggctgat gcgccaggcg
     1021 cggtcttcat agtgggcggg gaagatctcc agcgtgtcgc cccggcggcg gaacgtgccg
     1081 cgctcgaagg cggcgtcgtt gcgcttgtac tgctgggcca ccaggtcggc catcagctgc
     1141 ttctcgttga ccgtctggcc gaccttcagg gtgaaggtca tggccgtata ggtctcgacc
     1201 gagccgatgc cgtagatgca gctgaccgag gcgacgatga tcacgtcgtc gcgctcgagg
     1261 atcgcccggg tggccgagtg gcgcatccgg tcgatctgct cgttgatgga gctgtccttc
     1321 tcgatatagg tgtcggtgcg cgggacgtag gcttcgggct ggtagtagtc gtaatagctg
     1381 acgaaatatt cgaccgcgtt ctccgggaag aagctcttca tctcgctgta gagctgggcc
     1441 gccagggtct tgttgggggc caggatcagg gccgggcgct gggtcttctc gatgacctgg
     1501 gccatggtga aggtcttgcc cgagccggtg acgcccagca gcacctggtc ctggtcgccg
     1561 ttctccaggc cttccaccag ttcggcgatg gcggtcggct ggtcgccggc gggggagtat
     1621 tcggagacga gcttgaaccg cttgccgcct tccgacttgg cggggcgttc ggggcggtgc
     1681 ggggtccaca gggtctgccg ggcgatcatc gccgggtcgg acgagacgtc catgacgaag
     1741 ggcgtggaga cgtcggaaac gccgctatct c
//
LOCUS       DAOMEO010000738         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6883_length_1771_cov_2.511655, whole genome shotgun sequence.
ACCESSION   DAOMEO010000738 DAOMEO010000000
VERSION     DAOMEO010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_1771_cov_2.511655"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PLH31_14710"
     CDS             complement(<1..1411)
                     /locus_tag="PLH31_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HQR90481.1"
                     /translation="MTRLLERLAPCGPIIDVKAAERAHEAILKRVGEASASVEAAWLA
                     VAPVFAASPYLAGLARRDARRLPSILETDPDARLVEILAAAKAVAAEPDFETARRVLR
                     ELKADLHLLTALCDLGGVWDLDAVTGALTRFADATLNAALAQAVRLEVDRGALTHVGD
                     TEAGPAPGLFCIAMGKHGAFELNYSSDIDFSIFYAPEKLPVADGVEPQGVAVRIANHL
                     GRILQERTADGYAFRIDLRLRPDPSSTPPAMPVDAAMDYYESVGQNWERAAHIKARIA
                     AGDIRQGEAFLAELQPFIWRRNLDFAAIADIHSIKRQIHTYKVDDRLTAKGADLKLGR
                     GGIREIEFFVQTQQLILGGRQPDLRGPRTLDALAALAEAGHVTPEDAAWLTQSYKDLR
                     ALEHRAQMIADDQTHKLPESDTDRKKVAALWNHDNLRSFDAAVGKILKGVNLRYGRLF
                     AGEEELSSRFGSLVFTGVED"
     gene            1542..>1771
                     /locus_tag="PLH31_14715"
     CDS             1542..>1771
                     /locus_tag="PLH31_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQR90482.1"
                     /translation="MIELLVLALTRVQVAAAAAVLLVLLLRVPVRNLFGARLAYGLWS
                     LVPAAAAASVFPSLAETLGVGAPAAPLAPGLA"
BASE COUNT          270 a          572 c          637 g          292 t
ORIGIN      
        1 catcctcgac accggtgaag accaggctgc cgaaccgcga ggagagttcc tcctcgcccg
       61 caaacagccg gccatagcgc aggttgacgc ccttcaggat cttgccgaca gcggcgtcga
      121 agctgcgcag attgtcgtgg ttccacagcg ctgcgacctt cttgcggtcg gtgtcggact
      181 ccggcagctt gtgggtctgg tcgtcggcga tcatctgggc gcggtgctca agggccctta
      241 gatccttgta ggactgggtc agccaggccg catcttccgg cgtgacgtgg ccagcctccg
      301 ccagggcggc gagcgcgtcg agggtgcgag ggccgcgcag atccggctga cgcccgccca
      361 ggatcagctg ctgggtctgg acaaagaact cgatctcgcg aatgccgccg cgccccagct
      421 tcaggtcggc gcccttggcg gtcaggcggt catcgacctt gtaggtgtgg atctggcgct
      481 tgatcgagtg gatgtcggcg atggctgcga aatccaggtt ccgccgccag atgaagggct
      541 gcagctccgc caggaacgcc tcgccctgcc tgatgtcgcc cgccgcgata cgggccttga
      601 tatgggcggc gcgttcccag ttctgaccca cgctttcgta gtaatccatg gccgcgtcga
      661 ccggcatggc cggcggggtc gaggacggat cggggcgcag gcgcaggtcg atgcggaacg
      721 catagccgtc tgcggtgcgc tcctgaagaa tgcggcccag gtggttggcg atgcgcaccg
      781 ctacgccttg cggctcgacg ccatcggcca cgggcagctt ttcgggcgca tagaagatcg
      841 aaaaatcgat gtcgctggaa tagttgagct cgaaggctcc gtgcttgccc atggcgatgc
      901 agaacaggcc cggcgcgggt ccggcctcgg tgtctcccac atgggtgagg gcgccgcgat
      961 cgacctccag ccgcaccgcc tgggccaggg ccgcgttcag ggtggcgtcg gcgaagcggg
     1021 tcagggcgcc cgtcaccgca tccaggtccc agacgccgcc caggtcgcac agggcggtca
     1081 gcagatgcag gtcggccttc agctcgcgca gcacccggcg ggcggtctcg aagtcgggct
     1141 cggcggcgac agccttggcg gcggccagga tttcgacgag acgagcgtcc gggtcggtct
     1201 ccaggatcga cggcaggcgg cgggcgtcgc ggcgcgccag gccggccaga tagggcgagg
     1261 cggcaaacac cggcgccaca gcgagccagg cggcttccac gctcgccgag gcctcgccga
     1321 cacgcttgag aatcgcttca tgagcgcgct cggcggcctt cacatcaatg atagggccac
     1381 acggggcgag gcgttcgagc aggcgagtca tggcgccacc gtggcccgcc tcgcctgccc
     1441 cggcaaggca cacgcttgac gagcgatatt acagttgtaa ccatggctga ctgaaacccg
     1501 aggccagaga ccgggtcggg ccatgggcgg gggaagcgac gatgatcgaa ctgttggtcc
     1561 tggcgctgac acgggtgcag gtcgcggcgg cggcggcagt cttattggtc ctgctgctgc
     1621 gtgttcccgt tcgcaacctg tttggcgcgc gtctggccta cgggctctgg tcacttgtgc
     1681 cggcggcggc ggcggccagt gtctttccaa gcctggctga aacgctgggc gtgggcgccc
     1741 ccgccgcgcc gctggcgccg ggtctggcaa t
//
LOCUS       DAOMEO010000739         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6892_length_1769_cov_2.256126, whole genome shotgun sequence.
ACCESSION   DAOMEO010000739 DAOMEO010000000
VERSION     DAOMEO010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_1769_cov_2.256126"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>1383
                     /locus_tag="PLH31_14720"
     CDS             212..>1383
                     /locus_tag="PLH31_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR90483.1"
                     /translation="MSFTTQRPRARHALLAATALAGFVALPSLAMAQDSTVEEIIVTA
                     TKRDATILDVPFSINAQTQQDIQRSGAVTLEDLSRNVASLTIQNLGPGQSQVSVRGVS
                     AGQVVRDQPGVKEQVGVYLDESVISLSLFTPDIDLFDLNRVETLRGPQGTLFGSGSVG
                     GTIRYITNQPKIGVTEGVIEANGNMVDGDDTGGYVKGAINLPVSDKVAVRAVGYYTKY
                     AGFIDARTEGGGRKKNVNDGSRAGGRLAVLFQPTENISITPRVVYQEIRANGFNRQEA
                     FNLFANPNTITRAPVRLGEREQHLLLDESFADDTLLTDLTASIGLGAVELTSVTSYVD
                     RKIHLDRDASSLTGSVSVDLGFPSAAVLLPSKLVDTTDLQQFTQELRLGSTGDGPF"
     assembly_gap    1384..1393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1394..>1769
                     /locus_tag="PLH31_14725"
     CDS             <1394..>1769
                     /locus_tag="PLH31_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90484.1"
                     /translation="VFGGFYSEVDRVYDQRLPTPGYDAFTDATLGAGTSAAVANGFPL
                     NSPYNASLPYNIKQKALFGEASYKLDKLTVTAGGRFYDVEETRQFKSGGLFANGDNQV
                     DKTSSDGFTPRVLVAYEASDTVT"
BASE COUNT          322 a          621 c          528 g          288 t
ORIGIN      
        1 atgtttttaa tagcgacttg cacgcctggt gcccatggac agggtgacgc atggccgctg
       61 cgagccggat gtgagtcagc caagacacat cgccggacaa ttatccaatc ccgcccttga
      121 ttggcgttga ctttccgtcc ccggagcaag aacgcttaag ttgcggccga gccaggctca
      181 gagaccgcga aaaacgtctg ggaggatacc aatgagcttt acgactcagc gcccgcgcgc
      241 gcgtcacgcg ctgctggccg ccaccgccct ggcgggcttt gtggccctgc cctccctggc
      301 catggcccag gactcgaccg tcgaggaaat catcgtcacc gccaccaagc gcgacgcgac
      361 catcctcgac gtgccgttct cgatcaacgc ccaaacccag caggacatcc agcgctcggg
      421 tgcggtgacc cttgaagacc tgtcgcgcaa cgtcgccagc ctgacgatcc agaacctcgg
      481 ccccggccag agccaggtgt cggtgcgcgg cgtctcggcc ggccaggtgg ttcgcgacca
      541 gccaggcgtc aaggaacagg tcggggtcta tctcgacgaa tcggtgatct cgctgtcgct
      601 gttcacgccg gacatcgacc tctttgatct gaaccgggtg gaaaccctgc gtggcccgca
      661 gggcacgctg tttggctcgg gctcggtcgg cggcaccatc cgctacatca ccaaccagcc
      721 caagatcggc gttaccgagg gcgtgatcga ggccaatggc aacatggtcg atggcgacga
      781 caccggcggc tacgtcaagg gcgcgatcaa cctgccggtt tccgacaagg tcgccgtgcg
      841 cgccgtgggc tactacacca aatatgccgg cttcatcgac gcccgcactg agggcggcgg
      901 tcgcaagaag aacgtcaatg acggcagccg cgccggcgga cgcctggccg tgctgttcca
      961 gcccaccgag aacatcagca tcaccccgcg cgtggtctat caggagatcc gggccaacgg
     1021 cttcaatcgc caggaagcct tcaacctgtt cgccaatccc aacaccatca cccgcgcccc
     1081 ggtgcgcctg ggcgagcgcg agcagcacct gctgctggac gagagcttcg ccgacgacac
     1141 cctgctgacc gacctgaccg cctcgatcgg cctgggcgcg gtcgagctga cctcggtgac
     1201 cagctatgtg gaccgcaaga tccatctgga ccgcgacgcc agctcgctga ccggcagcgt
     1261 ctcggtggac ctgggcttcc ccagcgccgc cgtcctgctg ccctccaagc tggtcgacac
     1321 caccgatctg cagcagttca cccaggagct gcggctgggc tcgaccggcg atggcccgtt
     1381 ccannnnnnn nnnggtgttc ggcggcttct attccgaggt cgatcgcgtc tatgaccagc
     1441 gtctgcccac cccgggctat gacgccttca ccgacgccac cctgggcgcc ggcacctcgg
     1501 cggcggtggc caacggcttc ccgctgaact cgccctacaa cgcctccctg ccctacaaca
     1561 tcaagcagaa ggccctgttc ggcgaagcta gctacaagct ggacaagctg actgtcacgg
     1621 ccggcggccg cttctacgac gtcgaggaaa cccgccagtt caagtcgggc ggtctgttcg
     1681 ccaacggcga caaccaggtc gacaagacct cttccgacgg cttcacgccg cgggtcctgg
     1741 tagcctatga ggccagcgac acggtgact
//
LOCUS       DAOMEO010000740         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6895_length_1768_cov_2.241097, whole genome shotgun sequence.
ACCESSION   DAOMEO010000740 DAOMEO010000000
VERSION     DAOMEO010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_1768_cov_2.241097"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="sucA"
                     /locus_tag="PLH31_14730"
     CDS             <1..1065
                     /gene="sucA"
                     /locus_tag="PLH31_14730"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515717.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQR90485.1"
                     /translation="DWGTAEHLAFATLLDEGFPVRLSGQDSVRGTFTQRHSDVIDQTT
                     EEHYTPLNNIGPKQAHYEVIDSALSEEAVLGFEYGFSLADPNTLTLWEGQFGDFVNGA
                     QVVIDQFISSGERKWLRMSGLVMLLPHGYEGQGPEHSSARLERFLQSCAEDNMQVVNC
                     TTPANYFHALRRQMHREFRKPLIVMTPKSLLRHKRAVSNIADLAEGSSFHRVMIDGAE
                     AGCDVGGVTLKPDAEIKRVVICSGKVYFDLVDARAKSGRDDVYLLRLEQFYPWPMKSL
                     MGVLSRFKNAELVWVQEEPKNMGGWTFVDPWIELTLAKLDIKAKRARYIGRPASASTA
                     AGLMSRHLKELQAFLDETFA"
     gene            1160..>1768
                     /locus_tag="PLH31_14735"
     CDS             1160..>1768
                     /locus_tag="PLH31_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HQR90486.1"
                     /translation="MADIMTPALGESVTEATVARWTKKVGEAVKKDEILIELETDKVS
                     LEVASPADGVLSAIGAEEGATVVPGTVLGVVTEGATASAAPAAAAPKAAAPAPAPTPA
                     PAPAPVAAAAPAAATAAPVSPAPARVAAENNLDLSTVAGTGKDGRVTKGDALAALEAR
                     ANAPAPAAAPSAPRPIHEREERVKMTRLRQTIARRLKEAQNNA"
BASE COUNT          307 a          630 c          566 g          265 t
ORIGIN      
        1 gactggggca ccgccgagca cctggcgttc gccaccctgc tcgacgaagg cttcccggtg
       61 cgcctgtcag gccaggactc ggtgcgcggc accttcaccc agcgccattc ggacgtcatc
      121 gaccagacga ccgaggagca ctacacgccg ctgaacaata tcggtccgaa acaggcccat
      181 tacgaggtca tcgactcggc cctgtcggaa gaggcggtgc tgggcttcga gtacggtttc
      241 tcgctggctg atccgaacac cctcaccttg tgggaaggcc agttcggcga cttcgtgaac
      301 ggcgcacagg tggtgatcga ccagttcatc agctcgggtg agcgcaagtg gctgcggatg
      361 agcggcctgg tgatgctgct gccgcacggc tacgaaggcc agggtcccga gcacagctcg
      421 gcccgcctcg agcgcttcct gcagtcgtgc gccgaggaca acatgcaggt ggtcaactgc
      481 accaccccgg ccaattattt ccacgccctg cgccgtcaga tgcatcgcga gttccgcaag
      541 ccgctgatcg tgatgacgcc caagagcctg ctgcgccaca agcgcgccgt ctccaacatc
      601 gccgacctgg cggagggctc gtccttccac cgggtgatga tcgatggggc cgaggctggc
      661 tgcgacgtcg gcggcgtgac cctgaagccc gacgccgaga tcaagcgcgt cgtgatctgc
      721 tcgggcaagg tctatttcga tctggtcgac gcccgcgcca agagcggccg cgatgacgtc
      781 tatctgctgc gtctggaaca gttttatccg tggccgatga agtcgctgat gggcgtgctg
      841 tctcgcttca agaacgccga actggtctgg gttcaggaag aacccaagaa catgggcggc
      901 tggacgttcg tcgatccctg gatcgagctg acgctggcca agctggacat caaggccaag
      961 cgcgcgcgct atatcggccg tccggccagc gcctccacgg cggcgggtct gatgagccgc
     1021 cacctgaagg aactgcaagc cttcctcgac gagaccttcg cctagcttta tcgataaggc
     1081 cccgtccttc gcggcggggc cgtttccaca cctgccccgc caaacctgac cggcacaaac
     1141 gagagacatc ggaaccccca tggccgacat catgaccccc gccctcggcg aatccgtcac
     1201 cgaagcgacg gtcgcgcgct ggaccaagaa ggtcggcgag gcggtcaaga aggacgagat
     1261 cctcatcgag ctcgagaccg acaaggtcag cctggaagtg gcctcgcccg ccgacggcgt
     1321 gctgagcgcc atcggcgccg aagagggcgc gacggttgtt ccgggcacgg tcctgggcgt
     1381 tgtgaccgaa ggcgcgaccg cctcggccgc tcccgccgcc gccgctccga aggccgcagc
     1441 gccggctccc gcaccgaccc cggctcccgc gcccgctccg gtcgctgctg ctgcgccggc
     1501 cgccgccacg gctgctccgg tcagcccggc cccggcccgc gtcgccgccg agaacaatct
     1561 ggacctgtcg accgtcgccg gcaccggcaa ggacggccgg gtgaccaagg gcgacgccct
     1621 ggccgccctg gaagcccgcg ccaacgcccc ggcccccgcc gctgcgcctt cggccccgcg
     1681 tccgatccat gagcgcgaag agcgcgtgaa gatgacgcgc ctgcgtcaga cgatcgctcg
     1741 ccgcctgaag gaagcccaga acaacgcc
//
LOCUS       DAOMEO010000741         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6896_length_1768_cov_1.903094, whole genome shotgun sequence.
ACCESSION   DAOMEO010000741 DAOMEO010000000
VERSION     DAOMEO010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6896_length_1768_cov_1.903094"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>516
                     /locus_tag="PLH31_14740"
     CDS             <1..>516
                     /locus_tag="PLH31_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQR90487.1"
                     /translation="DEALALLGRNHDAGAARRAIAVAAATFPRLLVDLIYARPGQTVE
                     AWRAELEDVLAMGPEHVSPYQLTIEAGTAFDRAVGRGQLIVPDEDRAHDLFETTQTVL
                     EAAGFDAYEVSNHAKGEAARSRHNLVYWRGQDYVGVGPGAHGRLSLAEGRVATTAHRK
                     IVDYIAAVRDAG"
     assembly_gap    517..526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <527..692
                     /locus_tag="PLH31_14745"
     CDS             <527..692
                     /locus_tag="PLH31_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQR90488.1"
                     /translation="GVPFHEVAVLCLSADAAKVRHHVELGLLADDPRRLRATRAGRLV
                     LDRLTGALAT"
     gene            745..1629
                     /gene="rsmI"
                     /locus_tag="PLH31_14750"
     CDS             745..1629
                     /gene="rsmI"
                     /locus_tag="PLH31_14750"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQR90489.1"
                     /translation="MSRHPDPPALSDAPLAPGLYLVATPIGNLRDITLRALDVLNACD
                     VLLAEDTRVSGKLLAAYQISKRLERYDEHVADRVTPKILERLAAGERVAMVSDAGTPM
                     VSDPGFRLAREAIAAGHQVFPIPGASAALAALTLGGLPTDRFLFAGFPPPKSGARKTF
                     FEELAPVRATLIFYEGASRVADSLADMAAVFGPREAAVARELTKLYETCTRGTLDVLA
                     ADPRFESPKGEIVILVGPGSEAPVQAGDAESALREAMARLPLGEAASEVAKALGLPRR
                     DLYRQALAMKDAAQETAG"
     gene            1626..>1768
                     /locus_tag="PLH31_14755"
     CDS             1626..>1768
                     /locus_tag="PLH31_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90490.1"
                     /translation="MTGVRQIRGAKARLSGRRAEVLAAAWLMLKGYRILGFRLPTPLG
                     EIDL"
BASE COUNT          240 a          659 c          598 g          261 t
ORIGIN      
        1 cgacgaggcc ctggccctgc tgggccgcaa tcacgacgcc ggtgcggccc gacgcgcgat
       61 cgccgtggcc gccgccacct tcccgcgcct gttggtcgac ctgatctatg cccgccccgg
      121 ccagacggtc gaagcctggc gcgccgagct ggaagacgtg ctggccatgg gtcccgagca
      181 cgtctcgccg tatcagctga ccatcgaggc cggcacggcc ttcgaccgcg cggtggggcg
      241 cggccagttg atcgtccccg acgaggaccg ggctcacgac ctattcgaga ccacccagac
      301 cgtcctcgag gccgccggct tcgacgccta tgaggtgtcc aaccacgcga aaggcgaagc
      361 ggcgcggtcg cggcacaatc tcgtttactg gcgcggccag gattatgtgg gcgtcgggcc
      421 cggggcccat ggccggctga gccttgccga gggccgcgtg gcgaccaccg cccaccgcaa
      481 aatcgtcgac tacatcgccg ccgtgcgcga cgctggnnnn nnnnnncggc gtgcccttcc
      541 acgaggtcgc ggtgctgtgc ctctctgccg acgccgccaa ggtgcgccat cacgtggaat
      601 taggcctgct ggccgatgat ccgcgccgcc tgcgcgccac ccgggccggc cgtctggtgc
      661 tggaccggct gacaggcgcg ctcgcgacct gataggactg ggtcccgaac catcatcgcg
      721 tcccggcgcg gaaggatccc ccgcttgagc cgccaccccg acccgcctgc cctgtccgac
      781 gcgcccctgg cgccgggcct gtatctggtg gcgacgccga tcggcaatct gcgcgacatc
      841 accctgcggg ccctggatgt gctcaacgcc tgcgacgtgc tgctggccga ggacacccgc
      901 gtctcgggca agctgctggc cgcctaccag atctccaaac ggctggagcg ctatgacgaa
      961 catgtcgccg accgggtgac gccgaagatc ctggagcgtc tggcggccgg cgagcgggtg
     1021 gccatggtct ctgacgccgg cacgcccatg gtctccgatc cgggcttccg cctggcccgc
     1081 gaggccatcg ccgcagggca tcaggtcttt cccatacccg gcgcctcggc ggccctggcg
     1141 gccctgaccc tgggcggcct gcccaccgac cgcttcctgt tcgccggctt cccgccgccc
     1201 aagagcgggg cccgcaagac cttcttcgag gagctggccc ccgtgcgggc caccctgatc
     1261 ttctatgaag gcgcctcgcg ggtggccgac agcctggccg acatggccgc cgtcttcggg
     1321 ccgcgggagg ccgccgtggc ccgcgaactg accaagctct acgaaacctg cacgcgcggc
     1381 acgctggacg tgctggccgc cgatccccgc tttgaaagcc ccaagggcga gatcgtcatc
     1441 ctggtcggcc ccggctcgga agcccccgtg caagccggcg acgccgagag cgccctgcgc
     1501 gaggccatgg cccgcctgcc gctgggcgag gccgcctctg aagtggccaa ggccctgggc
     1561 ctgccgcgcc gcgatctcta tcgccaggcg ctggcgatga aggatgcggc ccaggagacg
     1621 gcgggatgac cggcgtccgc cagatcaggg gcgccaaggc gcgactgtct ggccgccgcg
     1681 ccgaggttct ggccgccgca tggctgatgc tgaagggcta tcgtattctg ggttttcgtc
     1741 tgcccacccc gctgggcgag atcgatct
//
LOCUS       DAOMEO010000742         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6899_length_1767_cov_1.812500, whole genome shotgun sequence.
ACCESSION   DAOMEO010000742 DAOMEO010000000
VERSION     DAOMEO010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_1767_cov_1.812500"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLH31_14760"
     CDS             complement(<1..390)
                     /locus_tag="PLH31_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HQR90491.1"
                     /translation="MTRHLLIVGAAFAALSLAACGDKTETKGAATPAEQAATPDANPA
                     ATVPTMADETKADVFAMKAADLNMFEIEAAKLAASRSTNAEVKAFAAKMETAHGKTGD
                     SLKAAIAASGAAITVPAMLSKDMQDDLD"
     gene            513..1709
                     /locus_tag="PLH31_14765"
     CDS             513..1709
                     /locus_tag="PLH31_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQR90492.1"
                     /translation="MARAPLDGLRVIEMGSLIAGPFCGQILGDFGAEVIKLEDPKVGD
                     PMRQWGRSKPQGLSPWWPVIGRNKKSVTIDLRTEEGRDLARSLIAQADVVVENFRPGT
                     MEAWGMGYEALSATHPGLVMARVSGFGQTGPYSHRAGYALVGEAMGGLRHITGEPDRN
                     PARAGISIGDSLSGLNAALGVMMALHSRDRTGKGQVVDAAIYESVLTVMENLITEYDL
                     TGYIRERSGAVLPGIAPSNVYPTKSGELILIGANQDTLFKRLCELMGRPDLVDDVRYR
                     DHAARGAHQGELDARIAAWTADQEIETLLPRLEEAGIATGRIYRAPDMLADPHFAARE
                     SIVSVPHPVFGQVKMQNAFPRLSQTPGAVRWTGPTLGEHTDAVLREVAGLSEEAIAGL
                     RARKVL"
BASE COUNT          269 a          553 c          636 g          309 t
ORIGIN      
        1 gtcgaggtca tcctgcatgt ccttggacag catcgccggc acggtgatgg ccgcgcccga
       61 ggcggcgatg gcggctttca ggctgtcgcc ggtcttgccg tgagccgttt ccatcttggc
      121 ggcgaaggcc ttgacctcgg cattggtcga gcgcgaggcg gccagcttgg cggcttcgat
      181 ctcgaacatg ttcaggtcgg cggccttcat ggcgaagacg tcggccttgg tctcgtcggc
      241 catggtcggg acggtggcgg ctgggttggc gtccggcgtt gcagcctgtt cggccggcgt
      301 cgcggcgccc ttggtttcgg tcttgtcacc gcaggcggcg aggctgaggg cggcgaaggc
      361 ggcgccgacg atgaggagat gacgggtcat ggctgtcttt ccctgcgcgt ttttatagag
      421 gattgaagtc tggtcagcgt cgcgcggcgc tgtatggcta taagaacatc acggcgccgt
      481 gatcggttcc gagcaggctg gggaagatcg acatggctag ggcgccgctg gacggattgc
      541 gggtcatcga aatgggctcg ctgatcgcgg ggcccttctg cggccagatc ctgggcgact
      601 tcggcgccga ggtgatcaag ctggaagacc ccaaggtcgg cgacccgatg cgccagtggg
      661 gtcgcagcaa gcctcagggc ctgtcaccct ggtggccggt gatcggccgc aacaagaaat
      721 ccgtcaccat cgacctgcgc actgaggaag gccgtgacct ggcccgtagc ctgatcgccc
      781 aggccgatgt cgtggtcgag aactttcgcc cgggcacaat ggaggcctgg ggcatgggct
      841 atgaggccct gtcggccacc catccgggtc tggtgatggc gcgggtgtcg ggctttggcc
      901 agacgggtcc ctattcacac cgcgccggct acgccttggt cggcgaggcc atgggagggc
      961 ttcgccacat caccggcgag cccgatcgca atcccgcgcg ggcgggcatc tcgatcggcg
     1021 acagcctgtc gggcctcaac gcggcgctgg gcgtgatgat ggccctgcat tcgcgggacc
     1081 gcaccggcaa gggccaggtt gtcgacgccg ccatctatga gagcgtgctg acggtgatgg
     1141 agaacctgat caccgaatat gacctcaccg gctatatccg cgaacggtcg ggcgccgtgc
     1201 tgcccggcat cgcgccatca aatgtctatc caacgaaaag cggcgagctg atcctgatcg
     1261 gggccaatca ggacaccctg ttcaagcgtc tgtgcgagct gatgggtcga ccggatctgg
     1321 tcgatgatgt ccgttatcgc gaccatgcgg cgcggggcgc gcatcagggc gagctggacg
     1381 cccgcatcgc cgcctggacc gccgaccagg agatcgagac cctgctgccg cgcctggagg
     1441 aagccggcat cgccaccggc cggatctatc gcgcgcccga catgctggcc gacccccact
     1501 tcgcggcgcg cgagtctatc gtcagcgtgc cccatccggt gtttggccag gtgaagatgc
     1561 agaacgcctt cccgcgcctg tctcaaaccc ccggcgcggt gcgctggacg ggcccgacgc
     1621 tgggcgagca taccgacgcc gtgctgcgcg aggtggcggg cctgagcgag gaggctatcg
     1681 ccgggctgcg ggcgcgcaag gttctctagc ttagatcctc cccctctggg ggaggtggcc
     1741 cagagggccg gagggggcca gctcggc
//
LOCUS       DAOMEO010000743         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6910_length_1765_cov_2.070760, whole genome shotgun sequence.
ACCESSION   DAOMEO010000743 DAOMEO010000000
VERSION     DAOMEO010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6910_length_1765_cov_2.070760"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1765)
                     /gene="ggt"
                     /locus_tag="PLH31_14770"
     CDS             complement(125..>1765)
                     /gene="ggt"
                     /locus_tag="PLH31_14770"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQR90493.1"
                     /translation="DAPAPTATTPRGFVAAANPLAAEAGLRVLREGGGAVDAAVAIQA
                     VLSLVEPQSSGLGGGAFLTYYDARTGKVTAYDGREKAPSGAAPDMFVGADGKPLPFVT
                     AVLSGRATGVPGAVAMLAQAQKDHGSARWSTLFKDAEQLATEGFVVSPRVAGMVNSPR
                     VPQGQTPDAKAYFTRPDGQRYKAGDVLKNPAYAATLRRLAAEGPSALYEGPIAQAMVD
                     KVREGALPGSLTLEDLKTYRPRSADALCRPWKVYTVCTPNPSSSGVAVIQALLMLEHT
                     QIGKLGPKDPAAWAILAEAERVMYADRDRYVGDPSFVKVPVEGLLDPAYVAERAKLIK
                     DVAGPAPKFGLPEGAPAAGADATQEPSGTSHFVVMDPAGNVVSMTTTVESIFGSGRMV
                     GGFFLNNQMTDFSFMPVEKDGAPAANAVAAGKRPRSSMTPLIVLDDKGRFLAAVGSPG
                     GNAILSYNLKAMVGLFYWGLTMQEAINLPNMVARGDNFSGDAELFGPDMVTALNARGV
                     TVKTGQNETSGLHGVIVRPGGALEGGADPRREGVARGL"
BASE COUNT          251 a          630 c          616 g          268 t
ORIGIN      
        1 aaacacacat cacggtcatc cgggttcgcc ccggccgctt cgcgcctccc caaccccttc
       61 agtggcgact gccgcgtgag gctgagaagc cgtgtccgcg cctgcctttc agggccccgg
      121 cgctctacag cccccgggcc acgccctcgc gacgggggtc agcgccgcct tccagagccc
      181 cgccgggccg tacgatcacg ccatgcagac ccgaggtctc gttctggccg gtcttgaccg
      241 tcacaccccg ggcgttgagg gcggttacca tgtcggggcc gaacagctcg gcgtcgccgg
      301 agaagttgtc cccgcgcgcc accatgttgg gcaggttgat ggcctcctgc atcgtcaggc
      361 cccagtagaa cagcccgacc atggccttga ggttatagga caggatggcg ttgccgcccg
      421 gcgagccgac ggcggccagg aagcggccct tgtcgtccag cacgatcagc ggcgtcatcg
      481 acgagcgcgg acgcttgccc gccgccaccg cattggccgc cggcgcgccg tccttctcga
      541 ccggcatgaa cgagaaatcg gtcatctggt tgttgaggaa gaagccgccg accatccggc
      601 ccgagccgaa gatgctctcg accgtggtgg tcatcgacac gacattgccg gccggatcca
      661 tgaccacgaa atggctggtg ccgctgggtt cttgggtggc gtcggcgccc gctgcgggtg
      721 cgccctcggg caggccaaac ttcggcgcag ggccggccac atccttgatc agctttgcgc
      781 gctcggccac ataggccggg tcgagcaggc cctcgacggg gaccttcacg aaggacggat
      841 cgccgacata gcggtcgcgg tcggcgtaca tcacccgctc ggcttcggcc aggatcgccc
      901 aggcggccgg atccttgggg ccaagcttgc cgatctgcgt gtgctccagc atcagcaggg
      961 cctggatcac cgccacgccg ctggacgagg gattgggcgt gcagaccgta tagaccttcc
     1021 aggggcggca cagggcgtcg gccgagcggg gccgataggt cttcaggtcc tccagggtca
     1081 ggctgccggg cagggcgcct tcgcgaacct tgtcgaccat ggcctgggcg atagggccct
     1141 catagagggc gctggggcct tcggcggcca ggcgcctgag cgtggccgca taggccggat
     1201 tcttcagcac gtcgccggcc ttgtagcgct ggccgtcggg ccgggtgaaa taggccttgg
     1261 cgtccggcgt ctggccctgg ggaacccgag ggctgttgac catgccggcc acgcgcgggc
     1321 tgaccacgaa gccctcggtc gccagctgtt cggcgtcctt gaacagggtg ctccaccggg
     1381 cgctgccgtg gtccttctgg gcctgggcca gcatggccac cgcgccgggc acgccggtcg
     1441 cgcggcccga cagcacggcg gtgacgaagg gcagcggctt gccgtcggcg cccacaaaca
     1501 tgtcgggcgc ggcgccgcta ggggctttct cgcgcccgtc ataggccgtc accttgccgg
     1561 tcctggcgtc gtaataagtg aggaacgcgc cgccgccgag gcccgagctc tgcggctcca
     1621 ccaggctcag caccgcctgg atcgccaccg ccgcgtcgac cgccccgccg ccctcgcgca
     1681 gcacccgcag gccggcctcg gccgccaggg gattggccgc agcgacaaag ccgcgcggcg
     1741 tggtcgccgt cggggccggg gcgtc
//
LOCUS       DAOMEO010000744         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6918_length_1763_cov_3.305621, whole genome shotgun sequence.
ACCESSION   DAOMEO010000744 DAOMEO010000000
VERSION     DAOMEO010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6918_length_1763_cov_3.305621"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLH31_14775"
     CDS             <1..359
                     /locus_tag="PLH31_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90494.1"
                     /translation="RITWTTLPFSKANHPPIARLDHDDRLTVTAGQTFVLSAKGSSDP
                     DGDSLSFHWFHYPEVGSWKTPISSEGADNIYRVAFRAPGVAKTETVHFVVAVSDKGAP
                     QLTRYRRVIVTIVPRS"
     gene            complement(396..>549)
                     /locus_tag="PLH31_14780"
     CDS             complement(396..>549)
                     /locus_tag="PLH31_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQR90495.1"
                     /translation="GRFSRLANAPAPFLPIDHVYAGGAWKTVSVVRGPALGSDHRPIV
                     VKLRRD"
     assembly_gap    550..559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(601..1443)
                     /locus_tag="PLH31_14785"
     CDS             complement(601..1443)
                     /locus_tag="PLH31_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR90496.1"
                     /translation="MDLLLRLLSLFFRGTGLMLGVVGAGFALACLGGVSSDRLDAFSH
                     FAPLWAAMGLGAILMGWIFARQGERWTIVGLGGATLFACAILMGPELLAAAGTKRLPA
                     RPDDLKVIQFNIWHENETPERALRWLLDQNADVVVIVEGGGMSGPAVKGLKAYYPFHV
                     TCDGKKRCGTMIFSRKRMIARNGFYPEGHDLAGAWATLADPKGEFTVVGIHYVWPMPA
                     GPQQQQSKRLTDAVARFEKSDMILTGDFNSTPWSFTLKRQDKALGLERRTRALPSWPS
                     GLLK"
     gene            complement(1483..>1763)
                     /locus_tag="PLH31_14790"
     CDS             complement(1483..>1763)
                     /locus_tag="PLH31_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90497.1"
                     /translation="SPTAENELGQVNHTVSFRLQADSYRVDAQEVVFRSKDPRVGEVV
                     FSGMFNVAALKAAKAAGPAGGAQTVLTGGLQIGAERIRNISFLYFAGD"
BASE COUNT          314 a          583 c          546 g          310 t
ORIGIN      
        1 cgcgtatcac ttggacgaca ctgcctttca gcaaggccaa tcatccgccg attgctcgtc
       61 tggaccatga tgatcgactg accgtcacgg ccggccagac cttcgttcta agcgccaagg
      121 gaagctctga tcctgacggc gacagccttt cgtttcactg gtttcactat cccgaggtgg
      181 gcagttggaa gacacccata tcctccgaag gcgccgacaa tatctatcga gtcgcgttca
      241 gggcccccgg tgtcgccaag acggaaacgg ttcatttcgt agtcgctgtc agcgacaagg
      301 gcgcgccgca actgacgcgc tatcggcgcg tgatcgtgac catcgttccc aggtcctagc
      361 agatatcgtc ggcggcacgc ggcccgcctg aaagcctagt cccgccgaag cttcaccacg
      421 atcggccggt gatctgagcc cagggccggc ccgcgcacga cgctgacggt cttccacgcc
      481 ccgccggcat agacgtggtc gatcggcagg aacggggccg gcgcattggc caggcgcgag
      541 aagcggcccn nnnnnnnnna gctttttgaa gcttttctgt ttcaactttt ttattccctt
      601 tcacttcaat agccccgaag gccaggaggg cagggcgcgg gtgcggcgct ccagccccag
      661 cgccttgtcc tggcgcttga gggtgaacga ccagggcgtg gagttgaagt cgccggtcag
      721 gatcatgtcg gacttctcga accgcgcgac ggcgtcggtc aggcgcttgg actgttgctg
      781 ctgcggcccg gcgggcatgg gccagacata gtggatgccc accaccgtga actcgccctt
      841 cggatcggcc agcgtcgccc aggcgccggc cagatcgtga ccttcgggat agaagccatt
      901 gcgcgcgatc atccgcttgc ggctgaagat catggtgccg cagcgcttct tgccatcgca
      961 ggtgacatga aacggataat aggccttcag gccctttacc gctggtcccg acatgccgcc
     1021 gccctcgacg atcaccacca catcggcgtt ctgatccagc agccagcgca gggcgcgttc
     1081 cggggtttcg ttctcgtgcc agatattgaa ctggatgacc ttcaggtcgt cagggcgcgc
     1141 tggcagccgc ttggttcccg cagcggccag cagttcgggt cccatcagga tcgcgcaggc
     1201 aaacagggtc gcgccgccca gtccgacgat ggtccagcgc tcgccctgcc gggcgaagat
     1261 ccagcccatc aggatcgcgc caaggcccat ggccgcccac aacggcgcga agtggctgaa
     1321 ggcgtccagc cggtcgctgg acacgccacc cagacaagcc agggcgaaac cggccccgac
     1381 aacgcccagc atcaggccgg tgccccggaa gaaaaggctc aagaggcgca gcaacagatc
     1441 catggcctgc ccttaacacg gtcgggcgcg acggcgcgcg tcttagtcgc cggcaaagta
     1501 gaggaagctg atgttgcgta tgcgctcggc gccgatctgc agcccgcccg tcagcaccgt
     1561 ctgtgccccg ccggctggac cggcggcctt cgcggctttc agggccgcta cattgaacat
     1621 gcccgagaag accacttcgc caacgcgtgg gtccttgctg cgaaacacca cttcctgggc
     1681 gtcgacccgg tagctgtcag cctgcaggcg gaagctgacg gtgtgattga cctggcccag
     1741 ctcgttctcg gccgtggggc tgc
//
LOCUS       DAOMEO010000745         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6948_length_1757_cov_2.308461, whole genome shotgun sequence.
ACCESSION   DAOMEO010000745 DAOMEO010000000
VERSION     DAOMEO010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_1757_cov_2.308461"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..640
                     /locus_tag="PLH31_14795"
     CDS             260..640
                     /locus_tag="PLH31_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HQR90498.1"
                     /translation="MTNKPLFASDAERMVETARLNDLARTAPKTVNATWLATSGVAAL
                     ISEAADGADRGVELIARLAGFTDFDEGNDPYGERDFGAFDLWGEQLFWKIDYYHPDRD
                     EHSPVKWSTELTRRVLTIMLASEY"
     gene            1155..>1757
                     /locus_tag="PLH31_14800"
     CDS             1155..>1757
                     /locus_tag="PLH31_14800"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR90499.1"
                     /translation="MAGQVRAGQGAAVTLTAFPGERLSCHVSAILPEATADCRTLTAR
                     IELPNRGGRLRPGMYGQAAFGGGATPALLVPSEAVIRTGRRTLVMLAEAGGRYRPAEI
                     QVGRESGDRTEVLAGLSEGERVVASGQFLLDSEASLSGLQARPAATGSPPSSPRPSLY
                     ETTGRIERLEASGVTLSHQPVPALQWPAMTMGFTLADPKLA"
BASE COUNT          253 a          621 c          596 g          287 t
ORIGIN      
        1 gggggaggtc tccccctgag cttgagccat ggggctcaat ctaccccctc tgcgggggca
       61 ccccgcaacg ccccgttggc taaggggccc cgcccctcgc gctcggaaga ccagccttgg
      121 gtgtcccctg ccttcctccc ctcggtcgtg caggtcgggc gatccccctc ccctcccccg
      181 gtcatccgct tgctaccccc gcctcgccgg cgggcagggg ttgatgcgca aggcatccaa
      241 ccctgaccgg agatcaaccg tgaccaacaa gcctctcttc gccagcgacg ccgaacgcat
      301 ggtggaaacc gcccgcctca acgacctggc gcgcaccgca ccaaagaccg tcaacgccac
      361 atggctggcg accagcggag tcgccgcctt gatctccgag gctgccgatg gggcggatcg
      421 gggcgttgaa cttatcgccc gcctggccgg gtttactgat ttcgacgagg gcaatgatcc
      481 ttacggcgag cgcgatttcg gggcgttcga cctatggggc gagcagctgt tttggaagat
      541 cgactactat catcccgacc gcgatgagca ctccccggtc aagtggagca ccgagctgac
      601 ccgccgcgtt ctgaccatta tgctggccag cgagtattga ccgggcgggc cagtctcctg
      661 gactggcccc ctcccctacc aaccgccttc ttggcgacga agactgctag gctcggcctt
      721 ggtcagaaat ccacggggat ggtgggccga gcggctcccc cacagagcaa gcgaggacca
      781 ccaaggcggc tgcgacttga gcaggcgccg ggcgcggtgg agttctggcc gtcgtctggc
      841 gctcgggttt gtacgtgcaa actttggctg cggggcggcg cgtcgcgccg ggcaagccgc
      901 gggcttgcta gccgccgatg gcggcaggcc ccgccggggc taggcgcggg atttgaccga
      961 tgaggcgcgt ggcctcgctt gggttcccag ggcccagcct tgcggcaagg cgtgacggtc
     1021 atttcctcca gcagcagtgt cggcgtctcg cgtgcgagac ccatcggtcc tttggccacg
     1081 caggcgtggc gtgttgctaa ggggcggtcg cccctcgcgc ccggggggta aagtctgcgc
     1141 tgcgctgcga agccctggct ggccaagtgc gtgctggaca gggtgccgcc gtgaccctga
     1201 ccgccttccc aggcgagcgg ttgtcgtgcc acgtctcggc tatcctgccg gaggccacgg
     1261 ccgactgccg cactctcacc gctcgcatcg agctgcccaa ccgcggcggc cgattgcggc
     1321 cggggatgta cggccaggca gctttcggcg ggggggccac cccggccctc cttgtgccgt
     1381 ccgaggccgt gatccgcacc ggccgacgca cgctagtgat gctggctgaa gccggcggac
     1441 gctatcggcc cgctgaaatc caggtcggcc gcgagagtgg cgaccgcacc gaggtcctgg
     1501 ccggcctcag cgagggggag cgggtcgtcg cttccggcca gttcctgctg gactccgagg
     1561 ccagcctttc gggcctgcag gcgagaccgg ccgcgaccgg ttcgccgcct tcgagcccgc
     1621 ggccatcgct ctacgagacc accggccgga tcgagcgcct cgaagccagc ggggtgacct
     1681 tgagccacca gccggtgccg gctctgcaat ggccggccat gaccatgggg ttcaccctgg
     1741 ctgacccgaa gctagcc
//
LOCUS       DAOMEO010000746         1757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6950_length_1757_cov_2.085194, whole genome shotgun sequence.
ACCESSION   DAOMEO010000746 DAOMEO010000000
VERSION     DAOMEO010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6950_length_1757_cov_2.085194"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLH31_14805"
     CDS             complement(<1..228)
                     /locus_tag="PLH31_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQR90500.1"
                     /translation="MNKPHHNTRGLLLMVVAPSGVGKTSLTRRLVADHGDLHLSISAT
                     TREPRPGEHDGRDYHFVSHEKFQEMIAADAFL"
     gene            complement(221..1099)
                     /locus_tag="PLH31_14810"
     CDS             complement(221..1099)
                     /locus_tag="PLH31_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQR90501.1"
                     /translation="MALSGMTGFARVEGAHGAWSWAVEARSVNGRNLETRFRGPPGFD
                     SLDRAARDGAQARFQRGQLTVNIQGKRAEAVGQTQVSVETLERYLKAGSAYVATGMVA
                     KPSLDGLLALRGVIEVREEDDDAETRAAIEAAMAGSIAQALDALKIARLEEGTALTPV
                     LHGQIDTLERLTTQAGAEAAGQPAVLKDRFSRRMTELLGEAASEERIVQEAAAMAVKA
                     DVREELDRLAGHVAARTLLTGDAAAGRRLDFLSQEFMREANTLCSKSALSSLTTIGLE
                     LKATIEQFREQVQNVE"
     gene            complement(1101..>1757)
                     /gene="mltG"
                     /locus_tag="PLH31_14815"
     CDS             complement(1101..>1757)
                     /gene="mltG"
                     /locus_tag="PLH31_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQR90502.1"
                     /translation="ANRLASNSLVECMVFARAAAQAIAKEATGTCPPLPTWDDSRVTD
                     PDGMDDRDQLLDELWPKRQKGLPFSTKDEAMTLASIVEKETGVASERPRVAAVFVNRL
                     RTGMRLGSDPTIIYGLTRGRPLGRGILLSELQRQTAYNTYLIDGLPPTPIANPGRAAI
                     AAVLDPPKTDELYFVADGTGGHVFARTLEEHNANVVKWRQIERSRAAAKKPAAPLSGG
                     "
BASE COUNT          245 a          571 c          636 g          305 t
ORIGIN      
        1 gaggaacgcg tccgccgcga tcatctcctg gaatttttcg tggctgacga agtggtagtc
       61 gcggccgtcg tgctcgcccg gacggggctc gcgcgtggtg gcgctgatcg acaggtgcag
      121 gtcgccatgg tcggcgacca gacggcgggt cagcgaggtc ttgcccacgc ccgagggcgc
      181 gacgaccatc agcaaaaggc cgcgagtgtt atggtggggc ttattcaacg ttctgaacct
      241 gttcgcggaa ctgctcgatg gtggccttca gctccagacc gatcgtggtc aggctgctga
      301 gcgccgattt cgagcagagg gtattggctt cgcgcatgaa ctcctgcgac aggaaatcca
      361 ggcggcggcc ggcggcggcg tcgccggtca gcagggtgcg ggcggcgacg tggccggcca
      421 ggcggtcgag ttcttcgcgc acatcggcct tcacggccat ggcggcggct tcctggacga
      481 tgcgctcttc agaggccgcc tcacccagca gttcagtcat ccgacgcgag aagcggtctt
      541 tcagcacagc gggctgaccg gcggcctcgg cgccggcctg ggtggtcagg cgctcaaggg
      601 tgtcgatctg gccgtggagc acgggggtca gcgccgtccc ctcctccagc cgcgcgattt
      661 tcagggcgtc cagcgcctgg gcgatggagc cggccatggc ggcctcaatg gcggcgcggg
      721 tctcggcgtc gtcgtcttct tcgcggactt cgataacgcc gcgcagggcc agcaggccat
      781 ccagactggg tttggcgacc atgccggtgg cgacataggc gctgcccgcc ttcagatagc
      841 gctccagggt ctcgacgctg acctgggtct ggcccacggc ctcggcgcgc ttgccctgga
      901 tgttgacggt cagctggccg cgctggaagc gggcctgggc gccgtcgcgg gctgcgcggt
      961 ccaggctgtc aaaaccgggc ggaccgcgga agcgggtctc gagattgcga ccattgacgc
     1021 tgcgagcttc cacggcccac gaccaggctc cgtgcgcgcc ttcgacgcgg gcgaagccgg
     1081 tcatgcccga cagcgccacc tcagcccccg ctcaggggcg cagccggctt cttggcggcg
     1141 gcgcgactgc gctcgatctg ccgccatttg acgacattgg cgttgtgctc ttccagggtg
     1201 cgggcgaaca cgtggccgcc cgtgccgtcg gcgacgaaat agagctcgtc ggtcttgggc
     1261 gggtccagta cggcggcgat ggcggcgcgg cccggattgg cgatcggcgt cggcggcagg
     1321 ccgtcgatca gataggtgtt gtaggccgtc tggcgctgca gctccgacag caggatgcca
     1381 cggcccagcg gccgtccccg ggtcaggcca tagatgatcg tcgggtcgct acccaggcgc
     1441 atgccggtgc gcaggcgatt gacgaacacg gcggccacgc gcggccgctc cgaggcgacg
     1501 ccggtttcct tctcgacgat ggaggccagg gtcatggcct cgtccttggt cgagaacggc
     1561 aggcccttct gacgtttcgg ccatagctcg tcgagcagtt ggtcgcggtc gtccatccca
     1621 tcggggtcgg tcacgcggct gtcgtcccag gtgggcagtg gcgggcaggt gcccgttgct
     1681 tccttcgcaa tggcctgcgc cgccgcacgc gcaaacacca tgcattccac aagtgaattg
     1741 ctggccagcc ggttggc
//
LOCUS       DAOMEO010000747         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6955_length_1756_cov_1.932981, whole genome shotgun sequence.
ACCESSION   DAOMEO010000747 DAOMEO010000000
VERSION     DAOMEO010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_1756_cov_1.932981"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLH31_14820"
     CDS             <1..593
                     /locus_tag="PLH31_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR90503.1"
                     /translation="PRTVGSEVAEIKTPPPAGSQPTDPASGLQIYRNEENQPSPTFAA
                     QPETPLPRPTAPVEAKPLTTAALPAAAKPAAAKPAPAPTIETLATAAIAPKPAAPKPV
                     AVATATAPKPAASASGPAMVQIGALSSPALADKAWNDAARLAPGLAAGKGKKVEAIDK
                     NGTTLYRTSVTGFASREAAKAFCDAILAAGKSCFVK"
     gene            604..>1128
                     /locus_tag="PLH31_14825"
     CDS             604..>1128
                     /locus_tag="PLH31_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR90504.1"
                     /translation="MSTSAAILGCAGTTLSREEEAFFRDVKPWGFILFKRNIADPEQV
                     RALTAALRDTVGRPDAPILIDQEGGRVARLQPPHWKTYPPGRAYGELVANDPLVAREI
                     TRLGARLIAHDLRAIGVNVDCVPVLDVPDPQGHEIIGDRAYGDTPEQVATLGRAAAEG
                     LLAGGVLPIIKHIPG"
     assembly_gap    1129..1138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1139..1622
                     /locus_tag="PLH31_14830"
     CDS             <1139..1622
                     /locus_tag="PLH31_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR90505.1"
                     /translation="SDSHLELPVVKAKLAELDARDFAPFRVLSDMPMAMTAHVVYTAI
                     DRRHPATTSKKVIKKIIRESLGFDGLLMSDDLSMKALSGHFKQRAKDSLSAGCDVVLH
                     CNGDMVEMKAVMSGVGGLSKEARRRSQAVMSRLVKVVEPFDVAEARARFDAAFDGRFA
                     "
     gene            1619..>1756
                     /locus_tag="PLH31_14835"
     CDS             1619..>1756
                     /locus_tag="PLH31_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQR90506.1"
                     /translation="MTTGFQPTLDFEAANAAAEDGAALVIDIDGYEGPLHVLLALARS
                     QK"
BASE COUNT          282 a          645 c          568 g          251 t
ORIGIN      
        1 cgccgcgcac ggtgggttcg gaggtcgccg agatcaagac gccgccgcct gcgggcagcc
       61 agccgacgga tccggcctcg ggcctgcaga tctaccgcaa cgaagaaaac cagccatcgc
      121 cgaccttcgc cgcccagccg gagacgccgc tgccgcgtcc gaccgcgccg gtcgaggcca
      181 aaccgctgac caccgctgct ctgccagccg cagccaagcc cgctgccgcc aagcctgcgc
      241 cggctcctac catcgaaacc cttgccaccg cggccatcgc gcccaagccc gccgcgccca
      301 agccggtggc ggtcgccacg gccacggcgc ccaagcccgc cgcctcggcc agcggcccgg
      361 ccatggtcca gatcggcgcc ctgtcctcac ccgccctggc cgacaaggcc tggaacgacg
      421 ccgctcgcct ggcccctggc ctggccgccg gcaagggcaa gaaggtcgag gcgatcgaca
      481 agaacgggac cacgctttac cgcacctcgg tgacgggctt tgccagccgc gaggccgcca
      541 aggcgttttg tgatgcgatc ttggccgcgg gcaagtcctg cttcgtgaag taggagcagg
      601 cccttgagca cctccgccgc gatcctcggc tgcgccggga ccaccctctc ccgcgaggaa
      661 gaggcatttt tccgggatgt gaagccctgg ggcttcatcc tgttcaagcg caacattgct
      721 gatcccgagc aggtgcgggc cctgaccgcc gccctgcgcg acacggttgg ccggcccgat
      781 gcgcccatcc tgatcgacca ggagggcggc cgcgtcgccc gcctgcagcc gccgcactgg
      841 aagacctatc cgcccggccg cgcctatggc gagctggtgg ctaacgaccc gctggtcgcc
      901 cgcgaaataa cccggctggg cgcacggctt atcgcccatg acctgcgagc catcggcgtc
      961 aatgtcgact gcgtgccggt gctggacgtg cccgatccgc aaggtcacga gatcatcggc
     1021 gaccgcgcct atggcgacac gcccgagcag gtggctaccc tgggccgggc cgccgccgag
     1081 ggcctgctgg ccggcggcgt gctgccgatc atcaagcaca tccccggcnn nnnnnnnnga
     1141 gcgacagcca tctggaactg ccggtcgtca aggccaagct ggccgaactg gacgcccgcg
     1201 acttcgcgcc gttccgcgtg ctgtccgaca tgccgatggc gatgacggcc cacgttgtct
     1261 acacggccat cgaccgccgt catccggcca cgacctcgaa gaaggtcatc aagaaaatca
     1321 tccgcgaatc cctgggcttc gacggcctgc tgatgagcga cgacctgtcg atgaaggccc
     1381 tgtcgggtca cttcaagcag cgcgccaagg acagtctgtc ggccggctgc gacgtcgtcc
     1441 tgcactgcaa tggcgacatg gtcgagatga aggccgtgat gtccggcgtc ggcggccttt
     1501 cgaaggaagc ccgccgccgg tcgcaggcag tcatgagccg gctggtcaag gtggtcgagc
     1561 cctttgacgt ggccgaggcc cgggcccgct tcgacgccgc ttttgacggc cgctttgcgt
     1621 gacgacgggc ttccagccca cgcttgattt cgaggccgcc aacgcggcgg cggaagacgg
     1681 cgcggcgctg gtcatcgaca tcgacggcta tgaaggcccg ctgcatgtcc tgctggcgct
     1741 cgcccgcagc cagaag
//
LOCUS       DAOMEO010000748         1755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6958_length_1755_cov_2.095882, whole genome shotgun sequence.
ACCESSION   DAOMEO010000748 DAOMEO010000000
VERSION     DAOMEO010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6958_length_1755_cov_2.095882"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLH31_14840"
     CDS             <1..284
                     /locus_tag="PLH31_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HQR90507.1"
                     /translation="DEPVASLDPKISAEVLRLLRDQAKSRGATVLCSLHQIDLARAFA
                     DRIIALGGGRVVFDGAPDQLSTSHLARIYGAAPLSNAMSDFTREPEPVS"
     gene            416..1297
                     /gene="phnE"
                     /locus_tag="PLH31_14845"
     CDS             416..1297
                     /gene="phnE"
                     /locus_tag="PLH31_14845"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HQR90508.1"
                     /translation="MLALSADGMLATVGLKDDSQVAKGFATIGHQMFPLVIAERTETS
                     RIENFDPEHLPPLARLETVESQVETLNPDTLQTDYSIERSQYLVEPFGYLSHVALKLI
                     ETLEIALWGTVLAVILAVPLAILGARNLTPHPVARVAARTVVSFFRSIPELISALFLV
                     LAYGFGPIAGVLALAFHAAGFLGKFYAEDIENTDPRAQEALGAIGAGRLKVLIYAILP
                     QVAPQYIAYTLYVLDRNVRMATVIGLVGAGGIGQELKGRYDMYNYGHVATILLAIFLL
                     VVALDQLSIRLRQRMLN"
     gene            complement(1550..1640)
                     /locus_tag="PLH31_14850"
     tRNA            complement(1550..1640)
                     /locus_tag="PLH31_14850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1604..1606),aa:Ser,seq:gct)
BASE COUNT          304 a          570 c          537 g          344 t
ORIGIN      
        1 ccgacgagcc ggtggccagc ctcgacccca agatcagcgc cgaagtgctc cgccttctga
       61 gggatcaggc caaatcccgg ggagccacgg ttctgtgcag ccttcaccag atcgacctgg
      121 cccgcgcctt tgcggatcgg atcatcgccc tgggcggcgg ccgcgtcgtg ttcgacggag
      181 cgccggacca gctatccaca tcccatctcg ccaggattta tggcgcggcg cccctgtcca
      241 acgcgatgtc tgacttcacg cgcgagccgg agcctgtgtc gtgaccccac cctcctccca
      301 agccccgcca gccgactggc gattttcacc gccttatgat gcgcgcagac tcgcactcgc
      361 gctgatcgcc gtcctggttc tgctgttcac gggtgcgcgg gtcgagatcg accggatgct
      421 ggccctgtcg gcggacggca tgctggcgac ggtgggcttg aaagatgact cccaggtcgc
      481 gaagggcttc gcgaccattg gtcatcagat gttcccgctg gtgatcgcgg agcgcaccga
      541 aaccagtcgc atcgagaatt tcgaccccga gcaccttccg cccctggccc gcctggagac
      601 ggtcgagagt caggtggaga cgcttaatcc agacacgctc cagaccgact actccatcga
      661 gcgctcccag tacctggtcg agccctttgg ctatctgagt catgtcgccc tgaaactgat
      721 cgagactctc gaaattgcgc tttggggaac tgtgctggcg gtcattctcg ccgtgccgct
      781 agcgatcctg ggggctcgaa acctgacgcc gcatcccgtg gcccgcgtgg ccgcccgtac
      841 ggtggtcagc ttcttccggt cgattccaga gttgatcagc gcgttgtttc tcgttctggc
      901 ctatggattc ggcccgatcg ccggcgtcct tgcgctagcg tttcacgccg ccggcttcct
      961 tgggaagttc tacgccgaag acatagaaaa cactgaccca cgggcccagg aagcgctcgg
     1021 cgccatcggt gcgggccggc tgaaggttct gatctatgcg atcctgccgc aggtggcgcc
     1081 gcaatacatc gcctataccc tctatgtcct cgaccggaac gtgcgcatgg ccaccgtcat
     1141 cggcctggtt ggcgccggcg gtatcggcca ggagctaaag ggccgctacg acatgtacaa
     1201 ctacggccat gtcgcgacga tcctgctcgc gattttcctg ctggtcgttg cgcttgacca
     1261 gctatcgatc cggctccgcc agcgcatgct gaactagggc tggcggccag ggcttttcaa
     1321 gaccaccagc cgcgaggcta ccgctccctc gtcggtgagc gcgtacaggt cgaagccgaa
     1381 gcggccattc gactcaagaa gcaaaatcct gcgggatttg gcgaccaatt gccttggccc
     1441 cataagtcgc tcgctttacg caggaccgtt gcggctaagc cgtgcctacc aacggcgcgg
     1501 gaagccatcc ggtcgagggg atgctgcgga ttacccgtgg aaacgggggt ggcggagacg
     1561 gagggattcg aaccctcgat acccttttgg ggtatgccgc tttagcaaag cggtgccttc
     1621 agcctctcgg ccacgtctcc tcctgagagg gagcggctgg atagcctgtt cgcagcgaca
     1681 tgacaatcac gcggaagcgg aaatcggtgc gttaacgcga agcttagacg ccgacgctag
     1741 gttgaacgct ggttt
//
LOCUS       DAOMEO010000749         1751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_6989_length_1751_cov_1.985259, whole genome shotgun sequence.
ACCESSION   DAOMEO010000749 DAOMEO010000000
VERSION     DAOMEO010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_1751_cov_1.985259"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLH31_14855"
     CDS             complement(<1..316)
                     /locus_tag="PLH31_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HQR90509.1"
                     /translation="MRHEKAARLLDLARMLAGSAEGMTLDEMARALDVGRRTAERMRD
                     AVWAAFPQMEVVDDPPTRRFRIPAGLDGLFQTPTAEELAALRTAADSYKASGAEGRAA
                     SLF"
     gene            388..732
                     /locus_tag="PLH31_14860"
     CDS             388..732
                     /locus_tag="PLH31_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008877606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90510.1"
                     /translation="MSRFACPAWAIAPINAPMISAVLAFADIHRDLGAGRTLMRISPE
                     RAARADVILLLGRDAERVVDLGLVWNDREDQIIRIIDDAEQHDSRLAAWEDAYDLFES
                     EVREPADHRLVA"
     gene            1004..>1751
                     /locus_tag="PLH31_14865"
     CDS             1004..>1751
                     /locus_tag="PLH31_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR90511.1"
                     /translation="MRGLSMNRHFKKTLIAGISALSFTIGYSAAAHAADTPVDPAAET
                     TSELETLIVTARGKPRTVLDSAVPVDSFSEADLKSAAFTDTNDILKTLVPSYTLAREP
                     ISDGATFIRPASLRGLPTDKTLFMVNSKRRHRSALVSIGGTGAQAPDAATIPASALKN
                     VVVTRDGAGAQYGSDAIAGVIDFQLKDSPSGGSLTAQYGQFFLGDGEDVMITGNLGLP
                     LGDSGFVNGTFEYTSQNQVHRGKQYCNKGIP"
BASE COUNT          336 a          606 c          512 g          297 t
ORIGIN      
        1 cgaacagcga ggcggcgcgc ccctcggcgc ccgacgcctt gtaggaatca gccgccgtcc
       61 gaagcgccgc cagttcctcg gcggtgggtg tctggaacag accgtccagt ccggccggga
      121 tgcgaaaacg gcgggttggc ggatcgtcga ccacctccat ctgcgggaaa gccgcccaaa
      181 ccgcatcgcg catgcgctcg gccgttcgcc gccccacgtc cagggcgcgc gccatttcgt
      241 ccagggtcat gccctcggcg gatcccgcca acatgcgggc cagatccagg agccgtgcag
      301 ctttttcgtg cctcatacgt tctccatacg accgaatctg gcgcacggca cgtgcaaaca
      361 gaaggggtcc gaacgaagga gacgtccatg tcccgctttg cctgccccgc ctgggccatc
      421 gccccgatca acgccccgat gatctcggcc gtcctggctt tcgcggacat ccatcgcgac
      481 ctgggcgccg gacgcaccct gatgcggatc agtcccgaac gcgccgcgcg agccgacgtc
      541 atcctgctgc tgggtcgcga cgccgaacgg gtggtcgatc tgggtctggt ctggaatgac
      601 cgcgaggacc agatcatccg gatcatcgac gacgccgaac agcatgattc ccgcctggcg
      661 gcctgggagg atgcgtacga tctgttcgag tccgaggtgc gcgaaccggc agatcaccgc
      721 ctcgtcgcct gacctgaccg tcgcaacgcc cgcgatctgg gcgatctgca agcaagtttc
      781 tgcctaaaaa tgcggtctcg acgccaccca aaggggcgcc ggggccgcag cgcgtttttg
      841 tgacgccttc ttgtcacggc tcccattgca gaagccatca tcttcactcc acgctgaatt
      901 ggtcggcaaa gtccgagtga aaagtcgcgt cagtaccaaa aataataaaa ggtcgggaga
      961 tgaacgccat ggaggaggaa tcaatcgtgc gttgataaat gaaatgaggg ggctctctat
     1021 gaatcggcac tttaaaaaga ctctgatcgc aggtatttcc gcgctatcat ttactattgg
     1081 atactctgca gcggcacatg ccgctgacac gcccgtcgat ccggcggctg aaaccacttc
     1141 cgaactcgaa accctgatcg tgacggcgcg cggcaagccc cgcaccgttc tggacagcgc
     1201 ggtcccggtg gactccttca gcgaggctga cctgaagtcc gcggccttca ccgacaccaa
     1261 tgacatcctg aagacgctgg tcccgtccta caccctggcc cgtgagccga tctcggacgg
     1321 cgccaccttc atccgccccg cgtccttgcg cggcctgccc accgacaaga ccctgttcat
     1381 ggtcaactcc aagcgccgac accgctcggc gctggtcagc atcggcggca ccggcgccca
     1441 ggcgcctgac gcggccacca tccccgcctc ggccctgaag aacgtggttg tcacccgcga
     1501 cggcgccggc gcgcagtacg gctcggacgc catcgccggc gtcatcgact tccagctgaa
     1561 ggacagcccc agcggcggtt cgctcaccgc ccagtacggc cagttcttcc tgggcgacgg
     1621 tgaagacgtg atgatcaccg gtaaccttgg cctgcccctg ggtgacagcg gcttcgtcaa
     1681 cggcaccttc gaatatacca gccagaacca agttcaccgc ggcaagcaat actgcaacaa
     1741 gggcatcccc a
//
LOCUS       DAOMEO010000750         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7002_length_1748_cov_1.828706, whole genome shotgun sequence.
ACCESSION   DAOMEO010000750 DAOMEO010000000
VERSION     DAOMEO010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_1748_cov_1.828706"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLH31_14870"
     CDS             <1..540
                     /locus_tag="PLH31_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQR90512.1"
                     /translation="AASAVGRGGRAGRDAARKLGATLAGFMVQDGGPRIAALSLDGWD
                     THAGQVGQINTRLTYLDAVLSGLNTGLGDEWKNTVVVTVTEFGRTARANGTGGTDHGT
                     ASTALVLGGALKPGGIIGDWPGLAQPALYENRDLAPTLDMRGLFKGLLGDHMGLDRAA
                     LESRVFANSAEARAVTGLV"
     gene            complement(544..1533)
                     /locus_tag="PLH31_14875"
     CDS             complement(544..1533)
                     /locus_tag="PLH31_14875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQR90513.1"
                     /translation="MKEVRTLYVDAGEDGTRVDRWFKRRWPHLNHIQLNKLFRSGQVR
                     VDGSRVKADTKLVAGSQVRVPPLPDAPDPDEKQALSKRDIAFAKSLVLYEDEEVLALN
                     KPAGLAVQGGTKTTHHIDKLLSAWGEGVNRPKLVHRLDRDTSGVLLLGKTPAAAARLS
                     GSFAKRKAQKTYWAIVQGNPHPVEGVIELHLAKRGVGDRELVVPAEPKDKDAQPAETE
                     FVSISRAGPRVTFMALRPHTGRTHQLRAHMKAIGHPILGDPKYSDEKSTQLSEGLKLQ
                     LHARSVMLPHPSVGMLFIEAPISPELKAGFAKFGFSEDEADQDPFAKKQIKRR"
     gene            complement(1530..1631)
                     /locus_tag="PLH31_14880"
                     /pseudo
     CDS             complement(1530..1631)
                     /locus_tag="PLH31_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671508.1"
                     /note="may be involved in chromosome condensation;
                     overexpression in Escherichia coli protects against
                     decondensation by camphor; overexpressing the protein
                     results in an increase in supercoiling; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
BASE COUNT          262 a          576 c          621 g          289 t
ORIGIN      
        1 gccgcgtccg ccgtcgggcg gggcggacgc gcgggccgcg acgccgcgcg caagctgggc
       61 gccaccctgg cgggcttcat ggtccaggac ggcggcccgc gcatcgcggc cctctcgctg
      121 gacggctggg acacccatgc cggccaggtg gggcagatca atacgcgcct gacctatctg
      181 gacgcggtgc tgtcgggcct caacacgggt ctgggcgacg agtggaagaa taccgtggtg
      241 gtcacggtca ccgaatttgg ccgcaccgcc cgcgccaatg gcacgggcgg caccgaccat
      301 ggcacggcct cgaccgccct ggtgctgggc ggcgcgctga agcctggcgg gatcatcggc
      361 gactggccgg gcctggccca gccggcgctt tacgagaacc gcgacctggc gccgaccctg
      421 gacatgcgcg gcctgttcaa gggcctgctg ggcgaccaca tgggcctcga tcgcgcggcg
      481 ctggagtcca gggtcttcgc caacagcgcc gaggcccgcg cggtgacggg actagtttag
      541 gccctagcgc cgcttgatct gcttcttggc gaacggatcc tgatcggcct cgtcttccga
      601 gaagccgaac ttggcgaagc cggccttcag ttcggggctg atcggggcct cgatgaacag
      661 catgcccacc gacgggtgcg gaagcatcac cgagcgggcg tgcagctgca gcttcaggcc
      721 ttccgacagc tgggtcgatt tctcgtcgct gtatttcgga tcgcccagga tcgggtggcc
      781 gatggctttc atgtgggcgc gcagctggtg cgtgcggccg gtgtgcggac gcagggccat
      841 gaaggtgacg cgcgggccgg cgcggctgat cgagacgaac tcggtctcgg ccggctgggc
      901 gtccttgtcc ttgggttcgg cgggaaccac cagttcgcgg tcgcccacgc cgcgcttggc
      961 cagatgcagc tcgatcaccc cctcgaccgg gtggggattg ccctgaacga tggcccaata
     1021 ggtcttctgc gccttgcgct tggcgaacga gcccgacagc cgcgcggcgg cggcgggggt
     1081 cttgcccagc agcagcacgc ccgaggtgtc gcggtccaga cggtggacca gcttgggccg
     1141 gttgacgccc tcgccccagg cgctcagcag cttgtcgata tggtgggtgg tcttggtgcc
     1201 gccctgcacg gccaggccgg cgggcttgtt cagggccagg acttcctcgt cctcgtacag
     1261 caccagcgac ttggcaaacg cgatgtcgcg cttggacagg gcctgctttt catctggatc
     1321 gggcgcgtcg ggcagcggcg gcacgcgcac ctgcgagccg gcgaccagct tggtgtcggc
     1381 cttcacccgc gagccgtcta cccgcacctg accggagcgg aacagcttgt tgagctggat
     1441 gtggttgaga tggggccagc ggcgcttgaa ccagcggtcc acccgggtgc catcctcgcc
     1501 cgcatccaca tagagcgtgc gtacttcttt catgccgcga acatcctgcg ggcgatcaga
     1561 aggcccgcga aaagggccgc cacggacaag agcacgctgg cggcggaata ggtgaaggcc
     1621 acacgatagg ccgcgctcgc catcaattta gtcatgcgcg cgcatacgat agccgactcc
     1681 gcgcaatgtc tcgatcgggt cgtggtcaaa gccctcgttg agcttggacc gcaaccggct
     1741 gagatggc
//
LOCUS       DAOMEO010000751         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7017_length_1743_cov_2.672393, whole genome shotgun sequence.
ACCESSION   DAOMEO010000751 DAOMEO010000000
VERSION     DAOMEO010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_1743_cov_2.672393"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLH31_14885"
     CDS             complement(<1..221)
                     /locus_tag="PLH31_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQR90514.1"
                     /translation="MSEAKIIDGKTFAADLREQIAEDVKALKAGYGVTPGLAVVLVGE
                     NPASQVYVRNKGEQTQAAGMHSETHRLPA"
     gene            313..1497
                     /gene="crtY"
                     /locus_tag="PLH31_14890"
     CDS             313..1497
                     /gene="crtY"
                     /locus_tag="PLH31_14890"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HQR90515.1"
                     /translation="MAPTASTSRADVVLVGGGLANGLIALRLKALRPDLRVIMLERDS
                     RIGGEHTWCHFETDVSPAISGWLRPLIVHRWSGYDVRFPGHQRSLETRYLAITSQRLH
                     EAVTAVLGSDVWLGATVSDVSPHQVTLSDGRRITAAAVIDGRGPRRSRSLVLGWQKFL
                     GQGVRLTAPHGLARPIVMDATVSQLDGYRFVYVLPLDATRLLIEDTRYSDGADLDHAE
                     LRAAIAAYAAQQGWTIAAVEREEHGVLPIALAGDIDVYWREARAQVAEVGLRAALFQP
                     TTGYSLPDAARLADQIAALPHITSAHVRTCVETHSKAAWRKRRYFRLLNRMLFKACAP
                     DQRYKVLERFYRLGGPLIQRFYAARLTPFDKARVLMGKPPVPIVAALGYLGESSVIED
                     KP"
     gene            1494..>1743
                     /locus_tag="PLH31_14895"
     CDS             1494..>1743
                     /locus_tag="PLH31_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR90516.1"
                     /translation="MSDPKPRAAVIGSGFGGLSLAIRLQSSGFDVTVIEGRDKPGGRA
                     YVWEDEGFIFDAGPTVITDPDCLKELFTISGRKIEDYVE"
BASE COUNT          271 a          607 c          553 g          312 t
ORIGIN      
        1 tcggccggca gacggtgggt ttcggagtgc atgcccgccg cctgggtctg ctcgcccttg
       61 ttgcggacat agacctggct ggccgggttc tcgccgacca gcaccacggc caggcctggc
      121 gtgacgccat agccggcctt cagggccttc acgtcttcgg cgatctgctc gcgcagatca
      181 gccgcgaacg tcttgccatc gataatcttg gcctcggaca tgggcgcctc ccggtttcgc
      241 tgtgcctacc gggtcgtttt gatgtgcgca acgctggcgt gaaggtcgca caatcatgtt
      301 agaggcaggg tcatggcgcc aaccgcttcc acctcccgag cagacgtcgt cctggtgggc
      361 ggcgggttag ccaacggcct gatcgccttg cggcttaagg ccctgcggcc agacctgcgc
      421 gtaatcatgc tggagcgcga cagccgaatc ggcggcgagc acacctggtg ccatttcgag
      481 accgacgtca gtcccgccat cagcggctgg ttgaggccgc tgatcgtgca tcgctggtca
      541 ggctacgatg tccgctttcc gggccatcag cgctcgctcg aaacacgcta cctggccatc
      601 acttcgcagc gcctccacga ggccgtgacg gccgtgctgg gctcggatgt ctggctcggc
      661 gcgacggtgt cggacgtctc gccgcatcag gtcacgctat ccgatggtcg ccgcattacc
      721 gccgccgccg tgatcgacgg cagaggcccg cgccgcagtc gcagtctggt gctcggctgg
      781 cagaagttcc tgggccaggg cgtgcgcctg acggccccac atggcctggc gcgacccatc
      841 gtgatggacg ccaccgtctc gcaactggac ggctatcggt ttgtctatgt cctgccgctc
      901 gacgccacgc gcctgctgat cgaggacacc cgctacagtg acggcgccga cctggaccat
      961 gcagaactgc gcgccgccat agccgcctat gccgcccagc agggctggac catcgccgcg
     1021 gtcgagcggg aggaacacgg cgtcctgccg atcgccctgg ccggcgatat tgacgtctac
     1081 tggcgcgagg cccgggcgca ggtggcggag gttgggctgc gggccgcact cttccagccg
     1141 acaacgggct actcccttcc cgacgccgca cgcctggccg atcagatcgc cgccctgccc
     1201 cacatcacca gcgcccatgt gcgcacctgc gttgaaaccc actccaaggc agcctggcgc
     1261 aaacggcgat acttccgcct gctgaaccgc atgctgttca aggcctgcgc gccagaccag
     1321 cgctacaagg ttctggagcg cttctaccgg ttgggcgggc cactgatcca acgcttctac
     1381 gccgcgcgcc tgacaccttt cgacaaggcg cgcgtcctga tgggcaagcc gcccgtacct
     1441 atcgtcgccg ccctggggta tctgggcgag agctccgtga ttgaggacaa accatgagtg
     1501 atcccaagcc gcgcgcggcc gtcatcggtt ccggcttcgg cggcctttcc ctggcgatcc
     1561 gcctgcagtc gtccggcttc gacgtcaccg tgatcgaggg tcgcgacaag cccggtggcc
     1621 gcgcctacgt ttgggaagac gaaggcttca ttttcgatgc cgggcccacg gtcatcaccg
     1681 atcccgactg cctgaaggag ctgttcacga tcagcggccg caagatcgag gactatgtcg
     1741 aac
//
LOCUS       DAOMEO010000752         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7046_length_1737_cov_2.360880, whole genome shotgun sequence.
ACCESSION   DAOMEO010000752 DAOMEO010000000
VERSION     DAOMEO010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7046_length_1737_cov_2.360880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLH31_14900"
     CDS             <1..188
                     /locus_tag="PLH31_14900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR90517.1"
                     /translation="GVGAALVKHGLSLHPSMTTDVNEQNGQAVGFYERMGFRRTGRSP
                     LDGQGRPYPLIHLAYGE"
     gene            complement(208..>428)
                     /locus_tag="PLH31_14905"
     CDS             complement(208..>428)
                     /locus_tag="PLH31_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90518.1"
                     /translation="PPQDVMECAARLLGVPVPPDLPFNEMGMSPATRRFYAENKRVSN
                     ARAKAELGWRLKYPTYREGLEAILRGGG"
     assembly_gap    429..438
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<439..1053)
                     /locus_tag="PLH31_14910"
     CDS             complement(<439..1053)
                     /locus_tag="PLH31_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HQR90519.1"
                     /translation="MRLFVFGLGYVGAAFAQALRVRGWAIAASARSPEQAAALRAAGI
                     EPADPNDRDAMASALTGVNAVLITSPPGPDGCVALESVIPALAQAQAFPDWIGYLSTT
                     GVYGDFEGRWVFETSPLKAQSVEGARRVGAERDWGQVGSGMGLTVATFRLPGIYGPGR
                     SALDRLRAGEGRRIVKPGQVFSRIHLDDIISGLLASLARPRAGGI"
     gene            1202..1276
                     /locus_tag="PLH31_14915"
     tRNA            1202..1276
                     /locus_tag="PLH31_14915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1234..1236,aa:Gly,seq:gcc)
     gene            1322..1396
                     /locus_tag="PLH31_14920"
     tRNA            1322..1396
                     /locus_tag="PLH31_14920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1354..1356,aa:Gly,seq:gcc)
     gene            1624..>1737
                     /locus_tag="PLH31_14925"
     CDS             1624..>1737
                     /locus_tag="PLH31_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQR90520.1"
                     /translation="MARITVLGECMVEMSPDGVSDDGAPRYRMGFAGDTFNT"
BASE COUNT          306 a          594 c          559 g          268 t
ORIGIN      
        1 cgggcgtagg tgcggctttg gtgaagcatg gtctgagcct ccatccctcg atgacgaccg
       61 acgtcaatga acagaacggt caagccgtgg gcttctatga gcgaatgggc tttcgacgca
      121 cggggcggtc gccgctcgat ggccagggta ggccctaccc gctgatccat ctggcgtacg
      181 gcgagtaact cctgacggag gccaccctca gccgccgccc ctcaaaattg cctccaaccc
      241 ctcgcgatag gtcggatact tcagccgcca gcctagttcc gccttggccc tcgcgttcga
      301 gacgcgcttg ttttccgcat agaagcgccg ggtggccggt gacatcccca tctcgttgaa
      361 cggcagatcc ggcgggaccg ggacgcccag cagcctggcg gcgcactcca tcacgtcctg
      421 cggcggggnn nnnnnnnnga tcccgccggc ccgggggcgg gccagggagg ccagcaggcc
      481 gctgatgatg tcgtccaggt ggatgcggct gaatacctgg cccggcttga cgatgcggcg
      541 gccttcgccg gctcgcaggc gatccagggc gctgcggccg gggccgtaga tgccgggcag
      601 gcggaaggtg gcgaccgtca ggcccatgcc gctgccgacc tgtccccagt cgcgctcggc
      661 ccccacacgg cgggcgccct cgacggactg ggcctttagc ggcgaggtct cgaacaccca
      721 gcggccctcg aagtcgccat agacgccggt ggtcgacaga taaccgatcc agtcggggaa
      781 ggcctgcgcc tgggccaggg cggggatcac cgattccagg gcgacacagc cgtccgggcc
      841 gggcggtgag gtgatcagca ccgcattgac ccccgtcaga gccgaagcca tggcgtcgcg
      901 gtcgttcgga tcggcgggct cgatcccggc cgcccgcagg gccgcggcct gctccggcga
      961 gcgcgccgag gcggcgatag cccagcccct aacccggagc gcctgggcga acgccgcccc
     1021 tacatagccc agaccgaaca cgaacagacg caagatccag ctccgccacc ccgaaaatca
     1081 gggggcgaat gagcccctga aaaaacgcgc tcggcaaccc ctcaaagggc gcttgccaag
     1141 gccggagcgc tctgccataa gccgcctcct cgtcgcgggg cgttcttttc gcttccacga
     1201 tgcgggcgta gctcagtggt agagcacaac cttgccaagg ttggggtcga gagttcgaat
     1261 ctcttcgccc gctccattcc tccactcacg gaggatggaa cgagacccga gaaatcacga
     1321 tgcgggcgta gctcagtggt agagcacaac cttgccaagg ttggggtcga gagttcgaat
     1381 ctcttcgccc gctccaattt ctcctgaaga ccccacacat gtccggtccg ccgagcgccg
     1441 caatggcgcg cctttcggcg gggtgaaacg atttgaagcc cttggcgaag ccgggcccgc
     1501 gaccatcaat ctcccttccc gcaaccggct cctgcaggcg gaagctctga tctgccccgc
     1561 catcctcgcg ccgactgcaa aaaccgccta ttaccgccgc aaacaggaaa gggaaccggt
     1621 gtcatggcca ggatcaccgt gctgggcgag tgcatggtcg agatgtcgcc cgacggagtg
     1681 tccgacgacg gcgcgccgcg ctatcggatg ggctttgctg gcgacacctt caacacc
//
LOCUS       DAOMEO010000753         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7050_length_1736_cov_2.844735, whole genome shotgun sequence.
ACCESSION   DAOMEO010000753 DAOMEO010000000
VERSION     DAOMEO010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7050_length_1736_cov_2.844735"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..539
                     /locus_tag="PLH31_14930"
     CDS             9..539
                     /locus_tag="PLH31_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HQR90521.1"
                     /translation="MLQAAETAPFANYATAYGKAMSVGMTSFGPLGWTSNASGYRYAD
                     THPGTGQPWPAMPQALLDLWTQLGDPDTPPDSCLINLYRGQARMGPHQDRDEADPRFP
                     VLSISLGDTAVFRIGGTSRKDPTRSLKLASGDVCRLSGPARLAFHGVDRILVSSSSIV
                     PDGGRINLTLRRATPA"
     gene            complement(620..>1736)
                     /gene="dnaA"
                     /locus_tag="PLH31_14935"
     CDS             complement(620..>1736)
                     /gene="dnaA"
                     /locus_tag="PLH31_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQR90522.1"
                     /translation="EPIEIVLPVSTDAPSLAPVPTGKPSRTQGLQERFTFETFVPGPA
                     NEFAHAVARRVASWADGHFNPVLFHGPYGFGKTHLLNALAWEAMRQAPTKRVVYLTAE
                     RFLSTFVRAVMDRQTAAFKEELRAADLLIIDDVHFIAGKQSTQEELFHTLTALVEEGR
                     RVVFSSDRPPSAMTEMDAHLRSHLSAGLVCGLEPADRSLRMGILERKIQTLSAAHGSD
                     PTMRPDVLQFLADRFTDSVRELEGALNTLSARAGEGLSRLTLEEVQAILRPHLRSGEK
                     RITIDDIQKATADHYGMKQADLLSERRNRAIARPRQAAMWLAKQLTTRSLPDIGRRFG
                     GRDHTTVLHAVRRIEALRAEDSALNHDLEVLTRKLRG"
BASE COUNT          273 a          575 c          617 g          271 t
ORIGIN      
        1 tcgccgacgt gctccaggcc gcagaaacgg cgccgttcgc gaactacgcc acggcctatg
       61 gcaaggcgat gagcgtgggc atgaccagtt tcggacctct gggctggacc agcaacgcca
      121 gcggctatcg ttatgccgac acccatccgg gcaccggcca gccctggccg gccatgcccc
      181 aggccctgct ggatctgtgg acgcagctgg gcgacccgga caccccgccc gactcctgcc
      241 tgatcaatct ctatcgcggt caggctcgca tgggtccgca ccaggatcgc gacgaagccg
      301 atccgcgctt tccggtgctg tccatctcgc tgggcgacac ggccgtgttc cggatcggcg
      361 ggaccagccg caaggacccg acccgcagcc tgaaactggc ctccggcgat gtctgccggc
      421 tgtcggggcc ggcgcgactg gcgtttcacg gcgtggaccg gatcctggtc agttcgtcca
      481 gcatcgtccc ggatggcggg cggatcaatc tgaccctgcg acgcgccacg ccggcctgaa
      541 cgaaaaaggg gccgccccat cgggcgaccc ctctcaatat ctaaccggac gcagacgccg
      601 ccaacaggcc cgcccaggcc tagccgcgca gcttgcgcgt cagcacttcc aggtcatggt
      661 tcagggcgct gtcctcggcg cgcagggcct cgatgcggcg cacggcgtgc aggacagtgg
      721 tgtggtcgcg accgccgaac cgccggccga tgtccggcag cgaccgggtg gtcagctgct
      781 tggccagcca catggcggcc tgacgcggac gggcgatggc gcggttgcgg cgctcgctca
      841 gcaggtcggc ctgtttcatg ccgtaatggt cggcggtggc cttctggatg tcatcgatgg
      901 tgatgcgctt ctcgcccgat cgcaggtgcg gccgcaggat ggcctgcact tcttccagag
      961 tcaggcgcga caggccctca ccggcccggg ccgacagggt gttgagcgcg ccttccagct
     1021 cacggacgct gtcggtgaag cggtcggcca ggaactgcag cacgtcgggc cgcatggtcg
     1081 ggtccgagcc atgcgcggcc gagagggtct ggatcttgcg ttccagaatg cccatccgca
     1141 gcgagcggtc ggccggctcc aggccgcata ccaggcccgc cgacaggtgc gagcgcagat
     1201 gggcgtccat ctcggtcatg gccgacggcg gacggtccga cgagaacacg acgcggcggc
     1261 cttcctcgac caaggcggtc agggtgtgga acagctcttc ctgcgtcgac tgcttgccgg
     1321 cgatgaagtg cacgtcgtcg atgatcagca ggtcggcggc gcgcagctct tccttgaagg
     1381 cggccgtctg gcgatccatc acggcgcgca cgaaggtcga caggaagcgc tcggcggtca
     1441 gatagaccac gcgcttggtc ggggcctgac gcatggcctc ccaggccagg gcgttgagca
     1501 ggtgggtttt gccaaagccg taggggccgt ggaacagaac ggggttgaag tggccgtcag
     1561 cccagctcgc cacccggcgc gccacggcat gggcgaattc gttggcggga ccggggacaa
     1621 aggtttcgaa ggtgaagcgt tcctgcaggc cctgggtgcg cgaaggcttg ccggtgggaa
     1681 ccggggccag gctgggggcg tcggtggaaa cgggcagcac gatctcgatc ggctcg
//
LOCUS       DAOMEO010000754         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7051_length_1736_cov_2.525877, whole genome shotgun sequence.
ACCESSION   DAOMEO010000754 DAOMEO010000000
VERSION     DAOMEO010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7051_length_1736_cov_2.525877"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>932)
                     /locus_tag="PLH31_14940"
     CDS             complement(<1..>932)
                     /locus_tag="PLH31_14940"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQR90523.1"
                     /translation="LEAMAAGVETVAALPDPLGVATARWTRPNGLDIARVRTPIGVIA
                     MIYESRPNVTADAAALCVRSGNAVILRGGSECLASSLAIHAAIVRGLKAAGLPASAVQ
                     AVGTSDRAAVGHILSGLNKTIDLIIPRGGKSLVARVQAEARAPVLGHLEGLNHVFVHA
                     GADPRKAIDIAVNAKMRRVSVCGSAETLLVDRAVADRLLPLLADALIKSGCELRGDAA
                     ALAIEPTMKKAKDADWTTEYLAPILSVAVVDGVAGAAAHIARYGSGHTDAIVTEDAAA
                     AEAFAAEVDSAIVLINASTQFADGGEFGFGAEIG"
     assembly_gap    933..942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<943..1093)
                     /gene="proA"
                     /locus_tag="PLH31_14945"
     CDS             complement(<943..1093)
                     /gene="proA"
                     /locus_tag="PLH31_14945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923275.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HQR90524.1"
                     /translation="MDDAGVSLQAKMIAMGEAARAGARALRLASAEQRTVALAAMAGA
                     IRHEAA"
     gene            complement(1274..1525)
                     /locus_tag="PLH31_14950"
     CDS             complement(1274..1525)
                     /locus_tag="PLH31_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90525.1"
                     /translation="MARFDKPAFDVTTAIAPPLPKTDTAMAAASRAAGAELQRRLKYQ
                     ALQCMLRAIETLEAVPEGDWRGRLEAVERLGREAEAEDR"
BASE COUNT          216 a          590 c          658 g          262 t
ORIGIN      
        1 tgccgatctc ggcgccgaag ccgaactcgc cgccgtcggc gaactgggtg gaggcgttga
       61 tcagcacgat ggcgctgtcg acctcggccg cgaaggcctc ggcggcggct gcgtcctcgg
      121 tgacgatggc gtcggtatgg cccgagccgt agcgggcgat atgcgccgcc gccccggcca
      181 cgccgtccac cacggccacc gacaggatcg gggccagata ttcggtggtc cagtcggcgt
      241 ccttggcctt cttcatggtc ggctcgatgg ccaaggcggc ggcgtcgccg cgcagctcgc
      301 agcccgactt gatcagggcg tcggccagca ggggcagcag gcggtcggcc acggcccggt
      361 cgaccagcag ggtctcggcc gagccgcaca ccgacacccg ccgcatcttg gcgttgacgg
      421 cgatgtcgat ggccttgcgc gggtcggcgc cggcatggac gaagacgtgg ttcaggcctt
      481 ccagatggcc cagcaccggg gcgcgggcct cggcctggac gcgggccacc aggctcttgc
      541 cgccgcgcgg gatgatcagg tcgatggtct tgttcaggcc gctcaggatg tggccgacgg
      601 cggcgcggtc ggacgtgccc acggcctgga cggcgctggc gggcaggccg gcggccttca
      661 ggccccgcac gatggcggcg tggatggcca ggctcgaggc caggcactcg gagccgccgc
      721 gcaggatcac cgcattgccc gaacgcacgc acagcgccgc cgcgtcggcg gtgacgttgg
      781 ggcggctctc atagatcatg gcgatcacgc cgatgggggt gcgcacccgg gcgatgtcca
      841 gcccgttggg ccgggtccag cgcgcggtgg ccacgcccag cggatcgggc agggcggcca
      901 cggtctcgac gccggcggcc atggcctcga gcnnnnnnnn nngggcggcc tcgtgtcgga
      961 ttgctccggc catggccgcg agggcgacgg tgcgttgctc ggccgaggcg agccggaggg
     1021 cgcgcgcgcc cgcgcgggcg gcctcgccca tcgcgatcat cttcgcctgc aagctgacgc
     1081 ccgcgtcgtc catccgcctc atcctcccag ggatatgctc aggttgagcc ttacctaggc
     1141 gctttcgccg cgagggggaa cctcggattg tctgatggcg gacgcaggtt cgttgcgcct
     1201 gccggtttgc gccagcggcg gcgaggcgtg tcctggccaa ggtatccccg cgagccgagc
     1261 agcgcctttt tcttcaacgg tcctcagctt cagcctcccg tcccagccgc tcgaccgcct
     1321 ccagcctgcc gcgccagtcg ccttcgggga cggcttcgag ggtctcgatc gcccgcaaca
     1381 tgcactgcag ggcctggtat ttcagccgcc gctgcagctc ggcgccggcc gcgcggctgg
     1441 ccgccgccat cgcggtgtcg gtcttgggga gcggcggcgc gatggccgtc gttacgtcga
     1501 aggcgggttt gtcgaaccgg gccatgggat gtcctctgag ggctgaagag agaaggctgc
     1561 gtcgccgcgt gacatgcgga cgcgcgtcgg cgccgaccgc caggacggac cgggcgggtg
     1621 gccccgggga aaagcgggga ggcggcggag cgtccgggcc aggcccggat gtgtttgtcg
     1681 tggagtaccg tttcggccgt ggccggagcg ctccgcgcgg ttgttggtca ggagca
//
LOCUS       DAOMEO010000755         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7059_length_1735_cov_2.350595, whole genome shotgun sequence.
ACCESSION   DAOMEO010000755 DAOMEO010000000
VERSION     DAOMEO010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_1735_cov_2.350595"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLH31_14955"
     CDS             <1..468
                     /locus_tag="PLH31_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HQR90526.1"
                     /translation="AMARLDAMDGVWRGPAWTLLADGRKHELTQTERIGPLLDGAIKV
                     LEGRGYEADGATSFNALGVISYEPSTDAFTLHSHAMSQKGDFSLKPTADGYVWEIPAG
                     PMVIRYTAVIRDGAWREIGERVMPDRPPMQFFEMNLKRVSDSAWPAAGAVAPR"
     gene            complement(493..864)
                     /locus_tag="PLH31_14960"
     CDS             complement(493..864)
                     /locus_tag="PLH31_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90527.1"
                     /translation="MPRPDAALDEAITAEERDLLRRIGDDDPGHIDQALSLLGSRSGW
                     ASSVLLVTQAVMFLAGLWSGWRFFEAQDMLTALHWGLPSAVLLIMALMLKLALWPAMQ
                     THRLLRALKRLELMIIQPRVD"
     gene            complement(857..1435)
                     /locus_tag="PLH31_14965"
     CDS             complement(857..1435)
                     /locus_tag="PLH31_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR90528.1"
                     /translation="MTPLNDSSNAQTRRLLDEYLVVAARAGDRRAFADLVRRWQQRLT
                     AHGWRLTGDRDMALDAVQAAWLEIARGLPRLEDERAFPAWAYRIVSRRCARDVTALKE
                     RRNLASAFAAEPEPEPHCPDPAAQDQARRLHVAIRTLPPGQRAALALYHFEGLSVGEI
                     AVALDVPAGTVKTRLMHARRTLRAVLGGDRNA"
     gene            complement(1512..>1735)
                     /locus_tag="PLH31_14970"
     CDS             complement(1512..>1735)
                     /locus_tag="PLH31_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR90529.1"
                     /translation="KVPTALHFGKSDPMIPMADVETIQAAHPDLPVWLYEAGHAFVAP
                     NGYHADSARLAMLRTLQLFQRGGGNRGEA"
BASE COUNT          297 a          563 c          617 g          258 t
ORIGIN      
        1 gccatggcca ggctggacgc gatggacggc gtctggcgcg gtccggcctg gaccctgctg
       61 gccgacggca ggaagcacga actcacccag accgagcgga tcggcccgct gctggacggt
      121 gccatcaagg tgctggaagg gcgcggctat gaagcagacg gcgccaccag cttcaacgcc
      181 ctgggcgtga tctcctacga gccctcgacc gacgccttca ccctgcactc ccacgccatg
      241 agccagaagg gcgacttcag cctcaagcct accgccgacg gctatgtctg ggagatcccg
      301 gccgggccga tggtcatccg ctatacggcc gtcatccggg acggcgcgtg gcgcgagatc
      361 ggcgaacggg tcatgccgga ccgtccgccg atgcagttct tcgagatgaa tctcaagcgc
      421 gtgagcgaca gcgcctggcc ggcggcgggg gcggtcgccc cgcggtaggg gcggcttgcc
      481 gcctgccgac cttcagtcga cgcgcggttg gatgatcatc aactcgaggc gcttgagggc
      541 ccgcaacagc cgatgggttt gcatcgcggg ccaaagggcg agcttcagca tcagcgccat
      601 gatcagcaga acagccgacg ggagacccca gtgcagggcg gtcagcatgt cctgggcctc
      661 gaaaaagcgc cagccgctcc agagcccggc gaggaacatc acggcctggg tgaccaggag
      721 cacgctgctg gcccatcccg agcgactgcc cagcaaggag agggcctgat cgatgtgacc
      781 gggatcatcg tcgccgatcc tgcgcaagag gtcgcgctcc tcggcagtga tggcctcgtc
      841 tagggcggcg tcgggtctag gcattgcgat ctcctccaag cacggcgcgc aaggtgcgcc
      901 gtgcgtgcat caggcgggtc ttgacggtgc ccgcgggaac gtcgagggcc acggcgatct
      961 cgccgacgct caagccctcg aaatggtaca gggccagcgc cgcgcgctgg ccgggcggca
     1021 gggtgcggat cgcgacgtgc aggcggcgag cctggtcctg tgcggcggga tctggacagt
     1081 ggggttcagg ctcgggttcg gcagcgaacg cgctggcgag gtttcgccgc tctttcaggg
     1141 cggtgacgtc gcgcgcgcac cggcgtgaga caatgcgata ggcccaggcc gggaaggctc
     1201 tttcgtcctc caaccgaggc aggccgcgag cgatctcgag ccaggcggcc tgaaccgcgt
     1261 ccagcgccat gtccctgtcg cccgtcagac gccagccgtg ggcggtcaac cgttgctgcc
     1321 accgccggac gaggtctgca aaggcgcgtc ggtcgccggc acgcgccgcg acgacgagat
     1381 attcgtccag cagtcgccgg gtctgcgcat tcgagctgtc gttcaacggg gtcatcgaag
     1441 gataggtggc gatccaacgg caaacggttc aaaaaaactg aaggcgttac cgttcaaagc
     1501 gccgaaccgg tctaagcctc gccccggttc ccgccgccgc gctggaacag ttggagggtg
     1561 cgcagcatcg ccagccgggc gctgtcggcg tgatagccgt tgggggccac gaaggcgtga
     1621 cccgcctcgt acagccagac cggcaggtcc ggatgggcgg cctggatggt ctcgacatcg
     1681 gccatcggga tcatcggatc ggacttgcca aagtgcaggg cggtcgggac ctttg
//
LOCUS       DAOMEO010000756         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7061_length_1735_cov_2.095238, whole genome shotgun sequence.
ACCESSION   DAOMEO010000756 DAOMEO010000000
VERSION     DAOMEO010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7061_length_1735_cov_2.095238"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLH31_14975"
     CDS             complement(<1..186)
                     /locus_tag="PLH31_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HQR90530.1"
                     /translation="MAHAADIDQHDAAHGADDKPPFFQRWFFSTNHKDIGTLYILFAI
                     MAGIVGGALSGLIRWELM"
     gene            complement(210..1136)
                     /gene="coxB"
                     /locus_tag="PLH31_14980"
     CDS             complement(210..1136)
                     /gene="coxB"
                     /locus_tag="PLH31_14980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQR90531.1"
                     /translation="MKAQFYGIRRMLTGASALAATMMFAGQALAQDLLGQPTPGAIDL
                     QPAASSLKRDAIWFHDIILMPIITVITLFVLGLLIWVVVRYNKKSNPVPAKFSHNTTI
                     EIVWTVVPVIILMVIAIFSFRLLFAYNDMPKPYMTVKATGYQWYWGYEYPDQKISEII
                     SIGLSEADSKAKGVPYRLAADNAMVVPVNQVVRVQVTGADVIHAFALPAFGLKTDAIP
                     GRLNETWFKAEKPGVYYGQCSELCGVDHAFMPIEIKVVSQAEFDAWVQSKTAPPAVAA
                     PVADAAAPAAAVATPASAPATTPAAAPAAPAV"
     gene            1492..>1735
                     /locus_tag="PLH31_14985"
     CDS             1492..>1735
                     /locus_tag="PLH31_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQR90532.1"
                     /translation="MPEAIEIQLKKGVLALCVLALLSRADSYAYEIASRLAKDIDMGE
                     GTIYPLMRRMQSDGLVETYLVESQSGPPRKYYRLTSA"
BASE COUNT          336 a          534 c          558 g          307 t
ORIGIN      
        1 catcagctcc cagcggatca ggcccgacag ggcgccgccc acgatcccgg ccatgatggc
       61 aaacaggatg tagagggtgc cgatgtcctt gtggttcgtg gagaagaacc agcgctggaa
      121 gaagggcggc ttgtcgtcag cgccgtgcgc tgcgtcgtgt tgatcaatgt ctgcggcgtg
      181 agccatcgga ctaatcctgc aatctggtct tagaccgccg gcgcggcggg agccgcggcc
      241 ggggtcgttg cgggggcgga ggccggggtc gcgacagcgg cagccggggc ggcggcgtca
      301 gcgacgggag cggcgacggc cggcggagcg gtcttggact ggacccaggc gtcaaactcg
      361 gcctgggaca cgaccttgat ctcgatcggc atgaaggcgt ggtccacgcc gcacagttcc
      421 gagcactggc cgtaatagac acccggcttt tcagccttga accaggtttc gttcaggcgg
      481 cccgggatgg cgtcggtctt caggccaaag gccggcaggg cgaaggcgtg gatcacgtcg
      541 gcgccggtga cctgcacgcg gaccacctga ttgaccggaa ccaccatggc gttgtcggcg
      601 gccaggcgat agggcacgcc cttggccttg gaatcggctt ccgacagacc gatcgagatg
      661 atctcgctga tcttctggtc cggatactcg tagccccagt accactgata gcccgtggcc
      721 ttcaccgtca tgtacggctt gggcatgtcg ttataggcga acagcagccg gaacgagaag
      781 atcgcgatca ccatcaggat gatcaccggg acgaccgtcc agacgatctc gatggtggtg
      841 ttgtggctga acttggccgg aaccgggttc gacttcttgt tgtagcgaac caccacccag
      901 atcagcagac ccagcacgaa cagggtgatg accgtgatga tcggcatcag gattatgtcg
      961 tggaaccaga tcgcgtcgcg cttcagcgac gacgcggccg gctggaggtc gatcgcccct
     1021 ggcgtcggct gacccagcag atcctgcgcg agggcctgcc ccgcgaacat catcgtggcg
     1081 gcgagggcgc tagcgcccgt cagcatccgc cgaatcccgt aaaattgcgc cttcatatcc
     1141 ctcacggccc gtttcagcga cgacctgccg ttaagataaa cgacacctcg cgccgacctg
     1201 catattacag ccaagcctgc ggttttgcgc aatcgactcc ttgtcacgga gtcgcggtcg
     1261 cagccgccca ttggcccttc cggtggctgc atacgcgtat cgtcagcggt tgccaagcgc
     1321 ctcagccccc cttgaaagcc aagggagcaa agggccggcg cataatttgc gaggcgcttt
     1381 caacaagcct gccccacggc ctccggttca ttacgtaaca ttcattgaca ggtaccttgc
     1441 accactcagt accttgccgt agaagagtgc atcaaccgcg gcgaggacaa cgtgccggaa
     1501 gccattgaaa tacaactgaa gaaaggcgtg ctggcgctgt gcgtgctggc cctgctgtca
     1561 cgggctgaca gctacgccta cgagatcgcc agcaggctcg cgaaggacat cgatatgggg
     1621 gagggaacaa tctatccgct gatgcgccgc atgcagtcgg acggcctggt ggagacctat
     1681 ctggtcgaat cccagagcgg tccgccgcgc aagtactacc gcctgacgtc ggctg
//
LOCUS       DAOMEO010000757         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7062_length_1735_cov_1.926190, whole genome shotgun sequence.
ACCESSION   DAOMEO010000757 DAOMEO010000000
VERSION     DAOMEO010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7062_length_1735_cov_1.926190"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLH31_14990"
     CDS             <1..571
                     /locus_tag="PLH31_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90533.1"
                     /translation="AVVAAPVVASAVPTAIAGLTATFPVLAAAIVVAVATGTALVAPI
                     LIGTPLIAASLPPLLTTSIRARPAATSVPVGLAARLLGLGSLGSLGRLLSRRRLGRRA
                     SAAATAPVGPAATAVATIAAIATRLGLLLTAWLLALPTALALATLTATAALTAAPVAA
                     AITARAVLALLALGSHRRSGPGFRRRAAQ"
     gene            713..1267
                     /locus_tag="PLH31_14995"
     CDS             713..1267
                     /locus_tag="PLH31_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HQR90534.1"
                     /translation="MTAHPMDIVGFWRSAGPRKWFAKDAGFDSMIALKFEQTHYRAAQ
                     RRYDKWAETPEGALALLILLDQFPRNLYRGTAHAFATDPLARMFARAALAAGHDQATE
                     PQLRPFFYLPFEHSESLADQEFCVELCATLQADSGDEDTIKWAVEHRDIIARFGRFPH
                     RNAVLGRETTPEEQAFLDEGGFAG"
     gene            1537..>1735
                     /locus_tag="PLH31_15000"
     CDS             1537..>1735
                     /locus_tag="PLH31_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQR90535.1"
                     /translation="MNFHTLHKARADTSHPEWQYLELLQDILDNGVQRGDRTGTGTLG
                     VFGRQIRFDLSKGFPVLTTKKL"
BASE COUNT          264 a          625 c          544 g          302 t
ORIGIN      
        1 cgcggtcgta gccgccccgg ttgtcgcgtc cgcggtccca accgccattg cgggcctgac
       61 cgccacgttt ccagtcctgg ccgccgcgat tgtcgttgcg gtcgccactg ggacggcctt
      121 ggtcgccccg attctgatcg ggacgcccct gatcgccgcg tccctgccac cgctcctgac
      181 cacctcgatc cgggcgaggc ccgcggccac ctcggttcca gtcggactgg cggcccggct
      241 gctgggcctg gggagcctgg ggagcctggg ccggttgctc agccgccggc gcctggggcg
      301 cagggcgagc gcggccgcca ccgctccagt cgggccggcc gccaccgcgg tcgccacgat
      361 cgccgcgatc gccacgcggt tgggcttgtt gctgaccgcc tggctgctgg ccctgccaac
      421 cgccctggcg ctcgccaccc tgacggccac cgccgcgctc accgccgccc cggtcgccgc
      481 cgcgatcacc gcgcgggccg tcctggcgct gctcgcgctg ggcagccacc gccgcagcgg
      541 acccggcttc cgccgccgcg ccgcccaata gcgacagagc catggcggtc gagatcagac
      601 gcttcatttc gtgctccaag aggccttgtg cgcggccccg gctttgtccc ttgataacca
      661 acatcggccg tcgcggatga accgcgtttg aacggataag ccagagcttc gcatgaccgc
      721 ccaccccatg gatatcgtag gcttctggcg ttcggccggc ccgaggaaat ggttcgccaa
      781 ggacgcgggg tttgattcga tgatcgccct gaagttcgag cagacccact atcgcgccgc
      841 ccagcgccgc tatgacaaat gggcggagac gccagagggg gcgctggcgc tgctgatcct
      901 gctggatcag ttcccgcgca atctgtatcg cggcaccgcc cacgccttcg ccaccgatcc
      961 cctggcccgg atgttcgccc gcgccgcgct ggccgctggc catgaccagg caaccgagcc
     1021 tcagctgcgg cccttcttct acctgccctt cgagcattcc gaatccctgg ccgaccagga
     1081 attctgcgtc gagctgtgcg ccaccctgca ggccgacagc ggcgatgagg acaccatcaa
     1141 gtgggcggtg gagcatcgcg atatcatcgc ccgcttcggc cgcttccccc atcgcaacgc
     1201 cgtgctgggc cgcgagacca cgccggagga gcaggcgttc ctggatgagg gcggcttcgc
     1261 ggggtgaagc tcagacggag ctagaccaga ggactccagt atctttttgc ttggcgctct
     1321 cgaatcgggt ggagcgattt cgaatcgcat cagagtttgc cgatccacag gccctgtggg
     1381 taactctgtt cacaatcaga tgttattcac aggctctgtt tatatgcggt gagcgcacgg
     1441 ctcgtactcg ttaagcgtgg tgtactgaac tgagtgcacc gtcgcgcgcg gacgcgaggc
     1501 gttgcaaacc gcacaggtca aacaggccgg tgcatcatga actttcacac cctccacaaa
     1561 gcccgcgccg atactagcca tcctgagtgg cagtatcttg agctccttca ggacatcctc
     1621 gacaacggcg tccagcgcgg cgaccgcacg ggcaccggca cgctgggcgt gttcggccgc
     1681 cagatccgct ttgacctgtc caagggcttc ccggtcctga ccaccaagaa gctgc
//
LOCUS       DAOMEO010000758         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7081_length_1731_cov_2.241647, whole genome shotgun sequence.
ACCESSION   DAOMEO010000758 DAOMEO010000000
VERSION     DAOMEO010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_1731_cov_2.241647"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLH31_15005"
     CDS             <1..384
                     /locus_tag="PLH31_15005"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90536.1"
                     /translation="IAHKWQADANPGADNKTLELTASVSRSIGPASARLLLQHSPEGA
                     GATRAWTWVEARLGWDLAPNLSASTAVGRREQSANIDYTGWNAGVTYAVNDRLDLDLR
                     WHDTDALAQGPQYAGALVAGLNVEF"
     gene            535..1113
                     /locus_tag="PLH31_15010"
     CDS             535..1113
                     /locus_tag="PLH31_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HQR90537.1"
                     /translation="MRKTISLIALGVASSIGAAAQAQAPNWNGPYQEPPRGSYVETCR
                     EITTFNGNVYARCKTSDGRWTWTGAHLRDCNNQGLANAGDGRLICQSLVGGGGRGGGR
                     PGGGGNAYRADAVLFEHAGYEGRPYEVRGDMPDLTAIKFNDVASSIRIMRGEWQVCEH
                     TNYQGRCWTLTRDEGLFNRDLNDRISSIRRVR"
     gene            1199..1336
                     /locus_tag="PLH31_15015"
     CDS             1199..1336
                     /locus_tag="PLH31_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HQR90538.1"
                     /translation="MRKIVVLAAAAAALLVSACNTVEGVGRDVSAAGRAVTDTARDVK
                     N"
     gene            complement(1342..1518)
                     /locus_tag="PLH31_15020"
     CDS             complement(1342..1518)
                     /locus_tag="PLH31_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90539.1"
                     /translation="MKRSQTTMPENGPAMPPEDDRPPAASRAEQAVRKASDQEIAPHE
                     KQAIAIETSREEDA"
BASE COUNT          310 a          576 c          552 g          293 t
ORIGIN      
        1 atcgcccaca aatggcaggc tgatgcgaat cccggcgccg acaacaagac actcgaactt
       61 accgccagcg tgagccgttc gatcggcccg gccagcgccc gcttgctact gcagcactcg
      121 ccggaaggcg caggcgcaac acgggcctgg acctgggtag aggccaggct gggctgggat
      181 cttgccccca acctgtccgc cagcaccgcc gtgggccgtc gcgagcagag cgccaatatt
      241 gactataccg gatggaatgc gggcgtgacc tatgctgtca atgaccgcct ggacctggat
      301 ctgcgctggc atgatacgga cgcgctggcg cagggtcctc agtatgcggg cgcgctggtc
      361 gccgggctga acgtcgagtt ctgatccccg gccgccgcca ggcaagccct cagatccgcg
      421 caccggaccg gcaatcgtgt cgccgtgaga agtagcgccc cgcgtgcgtt cagctacggt
      481 tcagccgtac ggcctagctt ggcttcataa agttcaaagc gtggggaaac cgcaatgcgc
      541 aagaccatca gcctgatcgc ccttggcgtc gcctccagta tcggcgcggc cgcccaggcc
      601 caggcgccca actggaacgg cccgtaccaa gagccgccgc gcggctccta tgtcgagacg
      661 tgccgcgaga tcaccacctt caatggcaac gtctatgcgc gttgcaagac cagcgatggc
      721 cgctggacct ggaccggcgc ccatctgcgc gactgcaaca accagggcct ggccaatgcc
      781 ggcgacggcc ggctgatctg ccagagcctg gtcggcggcg ggggtcgcgg cggcggccgt
      841 ccgggtggcg gcggcaacgc ctatcgcgcc gacgcggtgc tgttcgagca cgccggctat
      901 gagggccgcc cctatgaggt gcgcggcgac atgcccgacc tgacggccat caagttcaac
      961 gacgtggcct cgtccatccg gatcatgcgc ggcgaatggc aggtctgcga acacaccaac
     1021 tatcagggcc gctgctggac cctgacgcgc gacgagggcc tgttcaacag ggacctgaac
     1081 gacaggatca gctcgatccg ccgcgtgcgc tagaacgccg gccaaaaacc gcggcgacct
     1141 cttgatggct gccgcgcgcc atggctaagc tcccgcgcct tttgctggag cttagcgcat
     1201 gcgcaagatc gtcgtcctgg ccgccgcggc cgccgccctg ctggtctcgg cctgcaacac
     1261 cgtcgaaggc gtgggccgcg acgtctcggc cgccggccgc gcggtcaccg acacggctcg
     1321 cgacgtgaag aactagacgg ctcaggcgtc ttcttcgcgg gacgtctcta tggcgatggc
     1381 ctgtttttcg tgaggcgcga tctcctggtc cgacgccttg cgcaccgcct gttcggcgcg
     1441 cgaggcggcg ggcgggcgat cgtcttcagg cggcatggcg ggaccgtttt ccggcatggt
     1501 ggtctggctc cgtttcaagc acgatcctga aacgcggggc cgccgcccag gttgcacaga
     1561 gcatggctgc caagccaaag ccaccgaacg ccgattttga tagttaagat tacctgccac
     1621 gccgatattg ttcagatcat gttcgaactg aaacatatcg atacttgata atggacatgt
     1681 ttatgtccga ttaactaaca gatgcgtcgc taagtcacct ttcaacatgc g
//
LOCUS       DAOMEO010000759         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7083_length_1731_cov_1.683174, whole genome shotgun sequence.
ACCESSION   DAOMEO010000759 DAOMEO010000000
VERSION     DAOMEO010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7083_length_1731_cov_1.683174"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>806)
                     /locus_tag="PLH31_15025"
     CDS             complement(<1..>806)
                     /locus_tag="PLH31_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR90540.1"
                     /translation="GTTFQADHFRAKAKSAASVSLAGIIAPFLIAVLITPWLLKVPGL
                     FAAGISQFNATLFLGACIALTAFPMLARIINERGLAKTSLGTLSLTAGAFDDAVSWCV
                     LAVVLATFGGGAGVAILAVGGGLAYVLVLGLFGKRLLAPLGRMVEREGQMSLTVLAIT
                     LMLFCFSAFLMDAVGIHAIFGGFMLGAVMPRGLFVEELKKKVEPLAVVLLLPMFFTYS
                     GLNTRMDMVNSPQLLLIALGILLASIIAKGVACYAAARLAGEDNRTALGI"
     assembly_gap    807..816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<817..1150)
                     /locus_tag="PLH31_15030"
     CDS             complement(<817..1150)
                     /locus_tag="PLH31_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR90541.1"
                     /translation="MSDIIASTMQVAGKALAPNGPATLAAAHSYTPTDFSVHFFLQLA
                     VILLACRVVGWIGQKFLGQPQVVGEMIAGVVLGPSLLGLLFPAFQGMLFPKETKNVLY
                     VGAQLGVGL"
     assembly_gap    1453..1462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          333 a          553 c          573 g          252 t
ORIGIN      
        1 cgatgcccag ggcggtacgg ttgtcctcgc cggccagacg ggcggcggcg tagcaggcca
       61 cgcccttggc gatgatcgag gccagcagga tgcccagggc gatcagcagc agctgaggcg
      121 agttgaccat gtccatccgg gtgttgaggc ccgaataggt gaagaacatc ggcagcagca
      181 ggacgaccgc cagcggctcg accttcttct tcagttcctc gacgaacagg ccgcgcggca
      241 tgacggcccc cagcatgaag ccgccgaaga tggcgtggat gccgaccgcg tccatcagga
      301 aggccgagaa gcagaacagc atcagggtga tggccaggac cgtcaggctc atctggcctt
      361 cgcgctcgac catccggccc aggggcgcca gcaggcgttt gccaaacaac cccagcacca
      421 ggacataggc caggccgccg ccgacggcga ggatcgccac gccggcgccg ccgccgaagg
      481 tggccagcac caccgccagc acgcaccagg acaccgcgtc gtcgaaggcg ccggccgtca
      541 gcgacagggt gcccagcgag gtcttggcca ggccgcgctc attgatgatc cgggccagca
      601 tcgggaaggc ggtcagggcg atacaggcgc ccaggaacag ggtggcgttg aactgcgaga
      661 tcccggccgc gaacaggccc ggaaccttca gcagccaggg cgtgatcagc acggcgatca
      721 ggaaaggcgc gatgatgccc gccagcgaca cgctggcggc gcttttggcc ttggccctga
      781 agtgatcggc ctggaaggtg gtgccgnnnn nnnnnnagag gcccacgccc agctgggcgc
      841 cgacatacag cacgttcttg gtctcttttg ggaacagcat gccctggaag gccgggaaca
      901 gcagacccag cagggacggc cccagcacca cgccggcgat catctcgccc accacctggg
      961 gctggcccag gaatttctgg ccgatccagc cgacgacgcg gcaggccagc aggatcaccg
     1021 ccagctggag gaagaagtgc acgctgaaat cggtgggcgt gtagctatgg gcggccgcca
     1081 aagtggcggg accgttcggt gcaagggctt tccctgcgac ctgcatggtg ctggcaatga
     1141 tgtcagacaa gcgtacttcc cccgatcgcg gctcttgagg cctgcgtctc gcgacagaag
     1201 gggacaggct ttcgaaaggc gcgcaagccc agcgctgtca attttcccta ctcatcagaa
     1261 gattgtattt gctggtgaat tttctgttgc tcgacggcaa caaggtagta acgtacctac
     1321 aagacagcct tgtcattgta ggtatgtagt cacgtgaagg tcatcaccag tcgggcattc
     1381 aaccaggatg tcagccaggc caagcgcgcc gcgcggatcg caccggtgtt cgtcaccgac
     1441 cggggcaagg ccnnnnnnnn nngatgagcg tggaggccta tcgcaagctg atcggccaga
     1501 ccgagagcat cgtcgacctg ctggcgatgc cgggcctgga gcccatcgac ctgaccgccg
     1561 cccgctcggg ggcctgggac ggccggagcg gcgactgatg tacctgctcg acaccgacat
     1621 cgtcttcgaa ctgcgcaacg ccaagtccgg gggcgcatcg ccggccctgg ccgcctgggc
     1681 cagcggcgtc cagcgcgcca gcctgttcat ctcggccctg agcctgctgg a
//
LOCUS       DAOMEO010000760         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7099_length_1728_cov_2.080096, whole genome shotgun sequence.
ACCESSION   DAOMEO010000760 DAOMEO010000000
VERSION     DAOMEO010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_1728_cov_2.080096"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLH31_15035"
     CDS             complement(<1..658)
                     /locus_tag="PLH31_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90542.1"
                     /translation="MIRLGNVSLIALAMGASLALAGCGGADSVASPGEGSFAGGGTPT
                     TPTTPTTPAPGTPATDCPTGFANVGTVANGTLRNCQLPQSITGNLVVPLRDGTVYSVS
                     GRVDVGQDQGGDPNAPIAGRTKGVLTVEPGVKIFGSAGLDYIVVNRGSQIFAEGTATK
                     PIIFTSKQSVEGTTGIDSIGQWGGLVILGRAPTSNCSGSGATPGTANCEAQVEGTNAF
                     Y"
     assembly_gap    1026..1047
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1196..1516)
                     /locus_tag="PLH31_15040"
     CDS             complement(1196..1516)
                     /locus_tag="PLH31_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90543.1"
                     /translation="MLTVFAGFAAAAALSAGGAGADFVQAKSLASQAPAAKTLYICER
                     DAASKRTFKRLHGEVRFVTAKSLLARRGDGDQWSAPRCITGAELRRLESSSGNLIQIR
                     HQTR"
BASE COUNT          329 a          526 c          545 g          306 t
ORIGIN      
        1 cgtagaacgc gttggtgcct tcaacctggg cttcgcagtt ggcggtgccc ggcgtagcgc
       61 ccgagcccga gcagttgctg gtcggcgcgc ggccgaggat gaccagaccg ccccattgac
      121 cgatcgagtc aatgccggtg gtgccttcaa cgctttgctt gctggtgaag atgatgggct
      181 tggtcgcggt gccttcagcg aagatctgcg agccgcggtt gaccacgatg tagtccagac
      241 cggccgagcc aaagatctta acgcccggct caacggtcag cacacccttg gtgcgaccgg
      301 cgatgggcgc gttgggatcg ccgccctgat cttgaccaac atcgacgcgg ccgctgaccg
      361 aatacacggt gccgtcacgg agcggaacca ccaggttgcc ggtgatcgac tggggcagct
      421 ggcagttgcg cagcgtgccg ttggcgaccg tgccgacatt cgcaaagccg gtggggcaat
      481 cggtggcggg cgtgccgggc gccggggtcg tcggggtggt gggggtggtc ggcgtgccgc
      541 caccagcgaa ggagccttca cccggcgagg cgacgctatc ggcgccaccg caaccggcga
      601 gagccagaga ggcgcccatg gcaagcgcga tcaaactaac atttccgagg cggatcatca
      661 ggtttctcca gtggcgagca gccgcaaaca gggcagcgaa gaccgatggt cagcgcctat
      721 ggaaaagcct cgcccttcct cacccctgag gatgggctac agagcttgca cgacattcga
      781 cttacaattt tacgacggtt tgacgacgtg ccatttcagc cacgcacaaa ggccacgcct
      841 cggggcctcc agcgtcgcca gacaaatctg acgacagggt cagaaatgac caggccgaat
      901 tccagtctga acatggatgt tatctagcgg ccgacttaag gccctgcggg cctgctgtct
      961 gcgcatcacg ccggccaggc ctgctgttgt tcagcgcctg ctagccgtgc gcggcggcac
     1021 atccgnnnnn nnnnnnnnnn nnnnnnntgg cgccggtgtc cgcgacacga tcaggggccg
     1081 cggtcgccca gggcgcgcag aacagcgcgc agggttgtgg gccaaccgtc gatccgccgg
     1141 ggatcaagct ccaggcgcgt gaacttgaac gctcacgcgc ctggacgtgc tgaggttagc
     1201 gggtctggtg tctgatctgg atgagattgc cggacgacga ctccagccga cgcagctcgg
     1261 cgcccgtaat acagcgcggc gctgaccatt gatcgccatc accccgacgg gcgagcaaag
     1321 acttggcggt cacaaacctg acttcgccat gcagtcgctt gaaagttcgc ttggaagccg
     1381 cgtcgcgttc gcaaatatac agggtcttgg cggcaggcgc ctgtgacgca agactcttgg
     1441 cctgcacgaa atccgcgccc gcgccaccag cagacagggc ggcagccgcc gcaaacccag
     1501 caaaaacggt aagcatttct ctcctccttg ctcgattggt ccgagcttga aagagagctt
     1561 aggcccactt ttggaaatgt gaaggtattt tgacaagcct gtggataact tttcggcgac
     1621 agaatgacac agccgatttc aatccgacgg ctgagcaatt cctttcatgc ttctggcggc
     1681 ttcaaagccc ccgtgaccgt tgtcggatat ccgcatcccg tcgcttgg
//
LOCUS       DAOMEO010000761         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7105_length_1727_cov_2.747010, whole genome shotgun sequence.
ACCESSION   DAOMEO010000761 DAOMEO010000000
VERSION     DAOMEO010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_1727_cov_2.747010"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLH31_15045"
     CDS             complement(<1..121)
                     /locus_tag="PLH31_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR90544.1"
                     /translation="MAAKLSGGGGDRFNVEQNSEINVTPFVDVMLVLLIIFMVA"
     gene            complement(127..1023)
                     /locus_tag="PLH31_15050"
     CDS             complement(127..1023)
                     /locus_tag="PLH31_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR90545.1"
                     /translation="MLDIKRKTPLIALVGALALMASAPAFAQDAAAPAPAAAESAAVP
                     ATDAAAAPAPAPVEEPAAEEPAKAESHSLTPVGMFMQAGVVVKVVMIGLILASVFSWV
                     LLITKIFEFGALNKQSDKFLEAFRGARSIADIARIGSSEEFEGNPMADMAAAAAQEVE
                     LSRQAGLAVGGEHRDSTISRATYAINAVQASLVKRLSGGMVFLASVGSGGPFIGLFGT
                     VYGIMTSFIGIANTNTTNLAVVAPGIAEALLATGIGLFAAIPAVIFYNYFQTRISAYG
                     TRSEGFVAELMNAISRQLDKGA"
     gene            complement(1642..1717)
                     /locus_tag="PLH31_15055"
     tRNA            complement(1642..1717)
                     /locus_tag="PLH31_15055"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1682..1684),aa:Phe,seq:gaa)
BASE COUNT          339 a          518 c          556 g          314 t
ORIGIN      
        1 ccgcgaccat gaagatgatc aggagaacca gcataacgtc cacgaagggc gtaacgttga
       61 tctcgctgtt ctgttcgacg ttaaacctgt cgcccccacc gcctgagagt ttggcggcca
      121 tgggttttac gcccccttgt cgagctggcg cgagatggcg ttcatcagtt cagcaacaaa
      181 accttccgac cgcgtgccgt aggccgagat gcgggtctgg aagtagttgt agaagataac
      241 cgcggggata gcggcgaaca gaccgatacc ggtggcgagc agcgcttcgg cgatgccggg
      301 ggcgacgacg gccaggttgg tcgtgttggt gttcgcgatg ccgatgaacg aggtcatgat
      361 cccgtagacg gtaccgaaca gaccgatgaa cggaccgccc gaaccgaccg aggccaggaa
      421 caccatgccg cccgacagac gcttaaccag cgaggcttgc accgcgttga tggcgtaggt
      481 ggcgcgcgag atggtggagt cgcggtgttc gccgccgaca gccagaccgg cttgacgcga
      541 cagttcgact tcttgggcgg ccgcggcggc catatcggcc atcgggttgc cttcgaattc
      601 ttccgacgac ccgatgcggg cgatgtcggc gatcgagcga gcgccgcgga aggcttccag
      661 gaacttgtcc gactgcttgt tgagcgcgcc gaactcgaag atcttcgtga tcagcagaac
      721 ccacgagaag accgaggcga ggatcaggcc gatcatcacg accttaacga ccacgccggc
      781 ctgcatgaac atgccgacgg gggtgaggga gtggctttca gccttggcgg gttcttcagc
      841 ggcgggctct tcaaccggag ccggagccgg agcagcggcg gcgtcagtgg ccggaactgc
      901 ggcgctttcg gcggcggccg gggcgggagc cgcggcgtcc tgagcgaaag ccggggcgct
      961 tgccatcagc gcgagcgcgc caacgagagc gatgaggggg gtcttccgtt taatgtcgag
     1021 catctgtcgc cagttcctga ttggtctagc acgtttggac cgagggtcgt cgcgcgagag
     1081 cgtttctacc ctaactctga atagtccccc aggcggcaga tttctctgtc gacccggcgg
     1141 acccgcttcg ctgcgctcga cccgttacgc ccctcgcgag gaccaggccc tccagaagga
     1201 tcgtttcgaa gcgcggatga ccaaagcctc cggagaggct ctgaccttac cccaatgtta
     1261 gaattgcggc aggggtcctt tggcaacccc ttttcgtacc gtcaaggggt tcttctgggc
     1321 acaaaattat cgccccggcg acgttccacc cccgcttgct gcggcgcaca aaagggaaat
     1381 caggatttag gggcggtttt tccctgccaa cgggaatatc cacgctgcac tgcagcataa
     1441 gaacaaaacg ctggttggcg aaacgcgggc gcccagcgac gctccattgt gcgccatcat
     1501 gaaaggcgcg cgttctgcat atcggcctgg cagatcgata ttcaaggcgc cggctggacg
     1561 gccgccgcac ctccaaggga tctgacgacg gcgcgagatc gccggacgcg gcccatctgt
     1621 tctaaatcat tgaaaaagtg gtggtgcctg gagccggatt cgaaccagcg acacgcggat
     1681 tttcaatccg ctgctctacc aactgagcta tccaggccct tgggcct
//
LOCUS       DAOMEO010000762         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7110_length_1726_cov_3.035308, whole genome shotgun sequence.
ACCESSION   DAOMEO010000762 DAOMEO010000000
VERSION     DAOMEO010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7110_length_1726_cov_3.035308"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLH31_15060"
     CDS             <1..493
                     /locus_tag="PLH31_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQR90546.1"
                     /translation="TAYEQGVPIFCPAFVDSSAGFGLVKHQKERIAAKQPYLMIDAVA
                     DFRELTDIKIAAGTTGLFMVGGGVPKNFAQDTVVCAEILGVEADMHKYAIQITVADVR
                     DGACSSSTLKEAASWGKVNTTHEQMVFAEATTVVPLIASDAYHREAWKGRECPRWNKL
                     FGK"
     gene            607..1092
                     /locus_tag="PLH31_15065"
     CDS             607..1092
                     /locus_tag="PLH31_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQR90547.1"
                     /translation="MAHHAACDHDHDHHGVAGSALAAELEAAEARCVAADQRLTAPRR
                     RVLELLLEAGQPVKAYDLISTYAVDGPQAKPPTIYRALDFLEKQGFAHRIESLNAYVA
                     CRKEADGHAAAFLICDCCGATREIEPKASAEIIAAGAAAGYALTGVTIEAHGLCADCK
                     A"
     gene            1283..>1726
                     /locus_tag="PLH31_15070"
     CDS             1283..>1726
                     /locus_tag="PLH31_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR90548.1"
                     /translation="MTTAETLPGVEIWRGGVNTWDCDEMGHMNVRHYIVRAMEGLAGL
                     AAELGLPHAFTPYANATLLVKEHHVRFLREAHAGATLHMLGGVIEISDTEARLLQLLV
                     HSATGELAATFQTTVVHATPREGEVFPWPKATRERARAMMVEVPEK"
BASE COUNT          308 a          613 c          548 g          257 t
ORIGIN      
        1 gaccgcctat gagcagggcg tgccgatctt ctgcccggcc ttcgtcgaca gctcggccgg
       61 ctttggcctg gtcaagcacc agaaggaacg catcgccgcc aagcagccct atctgatgat
      121 cgacgcggtg gccgacttcc gcgaactgac cgacatcaag atcgcggccg gcacgacggg
      181 cctgttcatg gtcggcggcg gcgttcccaa gaactttgcc caggacaccg tggtctgcgc
      241 cgagatcctc ggcgtcgagg ccgacatgca caagtacgcg atccagatca ccgtggccga
      301 tgtccgcgac ggcgcctgca gctcctcgac cctgaaagag gccgccagct ggggcaaggt
      361 caacaccacc cacgagcaga tggtgttcgc cgaagccacc accgtggtgc cgctgatcgc
      421 ctcggacgcc tatcaccgcg aagcctggaa gggtcgcgag tgtccgcgct ggaacaagct
      481 gttcggcaag taagtcgata agctacgact gaaaatacag ggtccccgaa agcgatttcg
      541 gggacctttt tcatggacgc cgctctcgcg cttgcgaggg aggctccgcg cctctacctc
      601 ttggccatgg cccatcacgc cgcttgcgac cacgatcacg accaccacgg cgtcgccggc
      661 tccgccctcg cggccgagct ggaagccgcc gaggcccgct gcgtcgcggc ggaccagcgc
      721 ctgaccgcgc cgcgccgccg ggtgctggag ctgctgctgg aagccggcca gccggtgaag
      781 gcctatgacc tgatctcgac ctatgccgtc gatggccccc aggccaagcc gccgaccatc
      841 tatcgggcgc tggatttcct cgagaagcag ggcttcgccc accgcatcga gagcctcaac
      901 gcctatgtcg cctgccgcaa ggaggccgac ggccacgccg ccgccttcct gatctgcgac
      961 tgctgcgggg cgacccgcga gatcgagccc aaggccagcg ccgagatcat cgcggcgggc
     1021 gcggcagccg gctacgccct gaccggcgtg acgatcgagg cgcacgggct gtgcgcggac
     1081 tgcaaggcct gatccgaaaa ctttccatgt catcccggac aagcgcgcca gcgcgcagat
     1141 ccgggaccga cgcctgaacg ccgcgttcca agtcggtccc gggtcttcgc ccgggatgac
     1201 aacctttcgg tggtcttgat tgcggacctc acacgaaccc cgctagactc aaacgaccgt
     1261 tcaacccgcc gagacctcgc ccatgacgac cgctgagacc ctgcccggcg tcgagatctg
     1321 gcgcggcggc gtcaacacct gggactgcga cgagatgggg cacatgaatg tgcgccatta
     1381 catcgtgcgc gccatggagg gcctggccgg gctggcggca gagctgggcc tgccccacgc
     1441 cttcacgccc tacgccaacg ccaccctgct ggtgaaggaa caccacgtcc gcttcctgcg
     1501 cgaggcccat gccggcgcga ccctgcacat gctgggcgga gtgatcgaga tcagcgacac
     1561 cgaggcccgg ctgctgcaac tcctggtaca ttcggcgacg ggcgaactgg ccgccacctt
     1621 tcagaccacg gtcgtccacg ccacgccgcg cgagggcgag gtgtttccct ggccaaaagc
     1681 cacgcgcgag cgggccaggg ccatgatggt cgaggttccg gaaaag
//
LOCUS       DAOMEO010000763         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7113_length_1726_cov_2.194494, whole genome shotgun sequence.
ACCESSION   DAOMEO010000763 DAOMEO010000000
VERSION     DAOMEO010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7113_length_1726_cov_2.194494"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1399)
                     /locus_tag="PLH31_15075"
     CDS             complement(20..1399)
                     /locus_tag="PLH31_15075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HQR90549.1"
                     /translation="MTSRWTPATWRAKPAKHIPSDYPDAGAVERVEQTLRQMPPLVFA
                     GEARRLKTLLGDVAEGRAFLLQGGDCAESFKEFHADNIRDTFRLILQMAVVLTFAGGK
                     PVVKVGRIAGQFAKPRSEPIETIDGVTLPSYRGDNINGMDFTAEERIPDPDRLLKAYG
                     QSAATLNLLRAFANGGYADLHNIHRWTLGFVGDSPQGARYRELSDKITESLAFMAAIG
                     VTPQSHPGMHQVEFFTSHEALLLGFEEAMTRVDSTSGDWYDTSAHLLWIGERTRQLDG
                     AHIEFMRGVKNPIGLKCGPTMEGDDLLRLIDVLNPHNEPGRLTLYGRFGSDKIADRLP
                     RLMKATKAAGRSVVWAIDPMHGNTLKASTGYKTRPFDRILSEVKSFVEIANAEGVHPG
                     GVHLEMTGQNVTECLGGARAVSEGDLADRYHTHCDPRLNGEQALELAFLVAEKLKSAR
                     DDQRSLATG"
     gene            complement(1529..>1726)
                     /locus_tag="PLH31_15080"
     CDS             complement(1529..>1726)
                     /locus_tag="PLH31_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQR90550.1"
                     /translation="GGVFFSWVAYRIVALTGESREEGAIAAAALVTYLTIGALSFGVW
                     QEWWLALGAFTVIACDISRKD"
BASE COUNT          289 a          568 c          568 g          301 t
ORIGIN      
        1 agccaggaca accggcgcgt cagccggtgg cgagactacg ctggtcgtcc cgggccgact
       61 tcagcttctc ggccaccagg aacgccagtt ccagggcctg ctcgccgttc agacgcgggt
      121 cgcaatgggt gtggtaccgg tcggctagat cgccttccga caccgcccgc gcgccaccca
      181 ggcattcggt gacgttctgg ccggtcattt ccaggtgtac gccgccgggg tgaacgccct
      241 cggcgttggc gatctcgacg aaggatttca cctccgacag gatgcggtcg aacggccggg
      301 tcttgtagcc ggtcgaggcc ttcagcgtgt tgccgtgcat cgggtcgatg gcccagacca
      361 ccgagcggcc ggcggccttg gtcgccttca tcaggcgcgg caggcggtca gcgatcttgt
      421 cagagccgaa gcggccatac agcgtcaggc ggcccggctc gttgtgcggg ttgagcacat
      481 cgatcaggcg cagcaggtcg tccccttcca tcgtgggacc gcacttgagg ccgatcgggt
      541 tcttgacgcc gcgcatgaac tcgatgtgcg cgccgtccag ctgacgggtg cgctcgccga
      601 tccacagcag gtgggcgctg gtgtcgtacc agtcgcccga ggtgctatcg acgcgggtca
      661 tggcttcctc gaagcccagc agcagggcct cgtggctggt gaagaactcc acctgatgca
      721 tgcctggatg gctttgcggg gtcacaccga tggcggccat gaaggccagg ctctcggtga
      781 tcttgtcgct gagctcgcgg tatcgcgcgc cctgcgggct gtcgcccacg aagcccaggg
      841 tccagcggtg gatgttgtgc aggtcggcgt agccgccatt ggcgaaggcg cgcagcaggt
      901 tcagggtcgc ggccgactgg ccataggcct tcagcaggcg atccggatcg gggatgcgtt
      961 cctcggccgt gaagtccatg ccgttgatgt tgtcgccccg ataggacggc agggtcacgc
     1021 catcgatggt ctcgatcggc tccgagcgcg gcttggcgaa ctggcccgcg atgcggccca
     1081 ccttcaccac cggcttgccg ccggcgaagg tcagaaccac cgccatctgc aggatcaggc
     1141 ggaaggtgtc gcggatgttg tccgcatgaa attccttgaa gctctcggcg cagtcgccgc
     1201 cctgcagcag gaaggcgcgg ccctcggcca catcgcccaa cagggtcttc agacggcgcg
     1261 cctcgcccgc aaacaccagc ggcggcatct ggcgaagcgt ctgctcgacc cgttccacag
     1321 cgcccgcatc cggatagtcg gacgggatgt gcttggcggg ttttgctctc caggtggcgg
     1381 gggtccaacg cgaggtcatg actgggctct ataacttcaa agggagcggc ttttacccta
     1441 gccgcaacga aaaggcaaaa tcatgcggct gatcaccgct ttttgacaca ggcgaggaaa
     1501 gttcaccctt ccctcagccg tgacgacttc aatccttgcg cgagatatca caggcgatga
     1561 cggtgaacgc gccgagcgcc agccaccact cctgccagac cccaaagctc agggctccaa
     1621 tcgtcagata ggtgaccagg gccgccgccg ctatggcccc ctcctcccgg gactcgcccg
     1681 tcagggccac gatccggtag gcgacccagc tgaaaaagac gccgcc
//
LOCUS       DAOMEO010000764         1725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7115_length_1725_cov_2.632934, whole genome shotgun sequence.
ACCESSION   DAOMEO010000764 DAOMEO010000000
VERSION     DAOMEO010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7115_length_1725_cov_2.632934"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>635
                     /locus_tag="PLH31_15085"
     CDS             <1..>635
                     /locus_tag="PLH31_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90551.1"
                     /translation="SLRERDAGTVADLSTGTFGTARASGAGAVQIGQVRLMASGAYDR
                     GDGYTPVRGRARGAADTPVDLDARSAALRLDAPVGEAELSVRLGGYSEDRGAGLNGAR
                     AKATGSALSATLARTPSETGWGWRLQAWRRDSDLANSAVAVAANRATTTPANDQYETP
                     ATGQGVNAALRWSGPRFEGEFGLDARFADGEVRERFRYMAGAFTRDRRAGG"
     assembly_gap    636..645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <646..1611
                     /locus_tag="PLH31_15090"
     CDS             <646..1611
                     /locus_tag="PLH31_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90552.1"
                     /translation="ETSVAGAYAEGAVTQDQWLLAGGLRLDRWTSSNGLRIERDAANG
                     AVLLDDRPADRDGQVATARLGARRDLGQGGALRLAAYSGFRPPTLNELHRPFRVGNDI
                     TEANSALRPERLLGLETGVSARGQDYLLEATAFWNRIEDPIANVTLGAGPAVFPRAGF
                     VPAGGVLRERRNTGEITAIGLEAHATRKLGAALDVRAALSVTDARVDGGAAAPQLTGK
                     RPAQAPIWSATTGMQWRVSSRLDLDVDLTWEGKRFDDDLNSRVLGASLRIDARADWRL
                     ARGVTAYLAADNLLDEEIEVSRTANGVAGYANPRMARIGLRLAFD"
     gene            complement(1625..>1725)
                     /locus_tag="PLH31_15095"
     CDS             complement(1625..>1725)
                     /locus_tag="PLH31_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR90553.1"
                     /translation="LELAALFPRLDDRRLRRRVVDLVRAMVEEEPG"
BASE COUNT          260 a          603 c          609 g          243 t
ORIGIN      
        1 ttcactgcgc gagcgcgacg ccggaacggt tgcggatctc tcgaccggaa cgttcggaac
       61 ggcacgcgcc tcgggggcgg gggcggttca gatcggtcag gtgcggctca tggccagcgg
      121 cgcctatgat cgcggcgacg gctacacgcc ggtgcgcggc cgggcgcggg gcgcggctga
      181 cacccctgtc gacctcgacg cccgcagcgc cgccctgcgg ctcgacgctc cggtcggcga
      241 ggctgaactg tcggtgcggc tgggcggata cagcgaagac cggggcgcag gtctgaacgg
      301 cgcgcgcgca aaggccaccg gctcggctct cagcgccacg ctggcccgaa caccctctga
      361 aaccggttgg ggctggaggc tgcaggcctg gcgacgcgac agcgacctgg ccaacagcgc
      421 cgtcgccgtc gcagccaatc gcgcgaccac cacgccggcc aatgatcagt atgagacccc
      481 cgcgactggt cagggcgtca acgccgccct acgctggagc gggccccggt tcgaaggcga
      541 gtttggtctg gacgcccgtt tcgccgacgg cgaggtgcgc gagcgcttcc gttacatggc
      601 cggcgccttc acccgtgatc gtcgcgccgg cggtgnnnnn nnnnngagac ctcggtggcg
      661 ggcgcctatg ccgagggcgc cgtcacgcaa gatcaatggc tgctggccgg cggcctgaga
      721 ctggatcgct ggacctccag caacggcctg cggatcgagc gcgacgccgc caatggcgcg
      781 gttcttctgg atgatcgacc cgcagaccgc gacggtcagg tcgccaccgc ccgtctcggc
      841 gcacgccggg acctgggcca gggaggagcg ctgcggctgg ccgcctacag cggctttcgc
      901 ccgccgaccc tgaacgaact tcaccggccc tttcgcgtcg gcaacgatat cactgaagcc
      961 aattccgccc tgcggcctga gcggctgctt ggccttgaga ccggggtgtc cgcccggggc
     1021 caggactacc tgctcgaggc gacggcgttc tggaaccgga tcgaggatcc catcgccaat
     1081 gtcaccctcg gcgcggggcc ggcggtcttt ccgcgcgcgg gcttcgtgcc tgctggcggc
     1141 gtgctgcgcg agcggcgcaa taccggcgag atcaccgcca tcggacttga agcgcacgcc
     1201 acgcgcaagc ttggcgccgc gctggatgtc cgggccgctc tctccgtaac cgacgcccgg
     1261 gtcgacggcg gcgcggcggc gccccaattg acgggaaagc ggcccgctca ggcgccgatc
     1321 tggagcgcca cgacgggcat gcaatggcgc gtttccagcc ggcttgatct ggacgtcgat
     1381 ctcacctggg agggaaaacg gttcgacgat gatctcaaca gccgagttct gggcgccagt
     1441 ctgcggatcg acgcgcgcgc cgactggcga ctggccagag gcgtcacggc ctatctggcg
     1501 gctgacaacc tgctggatga agagatcgag gtctcgcgca cggccaacgg cgtggccggc
     1561 tacgccaatc cccgcatggc ccggatcggc ctccggctgg cgttcgactg aagcgcggcg
     1621 aggctcagcc cggctcttcc tcaaccatgg cccggaccag atccaccaca cgccggcgca
     1681 ggcgtcggtc atcgagccgc ggaaacaggg cggccagttc caggc
//
LOCUS       DAOMEO010000765         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7118_length_1724_cov_2.804673, whole genome shotgun sequence.
ACCESSION   DAOMEO010000765 DAOMEO010000000
VERSION     DAOMEO010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7118_length_1724_cov_2.804673"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLH31_15100"
     CDS             <1..269
                     /locus_tag="PLH31_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQR90554.1"
                     /translation="HGKFLTRLWDDGDTLVCPGVPFDEREMLMEAEPQTFYLTDHYRN
                     YPYVLVRLSQVHAGTVERLIQRHWRATAPKKVLKAWDAQHPETA"
     gene            266..910
                     /locus_tag="PLH31_15105"
     CDS             266..910
                     /locus_tag="PLH31_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HQR90555.1"
                     /translation="MTAWRILIAVLAWTGVTIQYGLMIHNESWASGPWKTVEFFSYFT
                     ILSNLLAGVVLTAPLVAPASRLAAWAEASSTRIAAATYLAITALVYHGLLADIWDPKG
                     LGLVADTLLHTVTPIAVLADWLLRGGKGPARWSGAFKALAFPALFGVWTLLHGALTDF
                     YPYPFLDVTKRGYPGVMVMMLAMSAGFLVVAVAFTVLHRARARLAIDRRAPLSA"
     gene            931..>1724
                     /locus_tag="PLH31_15110"
     CDS             931..>1724
                     /locus_tag="PLH31_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR90556.1"
                     /translation="MSKRVPAVRSGDVLIPALGFGTWQLDHGTAQPLVETALEIGYRH
                     IDTAQAYRNERDVGAAIAASGIRRDDIFLTTKVWIDQFAAGDLQRSAEKSLEKLGVDH
                     VDLLLLHWPKPDVPLAETMAALNAVRERGLTRAIGLSNFPSALMAQAQTLSAAPIATN
                     QVEYHPYLSQKTLIAAAHAAGTSITAWSPLAQGKVAEDPVLIAIGQAHGKAPGQVTLR
                     WLIQQGVIAIPRTKTPARIAENFDVLDFALSDDEMAAVFALARPDGR"
BASE COUNT          260 a          641 c          547 g          276 t
ORIGIN      
        1 cccatggcaa gttcctgacc cgcctgtggg acgacggcga caccctggtc tgccccggcg
       61 tgccgttcga cgagcgcgag atgctgatgg aagccgagcc gcagaccttc tatctcaccg
      121 atcactaccg caactatccg tatgtgctgg tccgcctgtc gcaggttcac gccggaacgg
      181 tggaacggct gatccagcgg cactggcgcg ccacggcgcc caagaaggtc ctgaaagcct
      241 gggacgccca acacccggag accgcatgac cgcctggcgc atcctgatcg ccgtcctggc
      301 ctggacgggc gtgaccatcc agtacggcct gatgatccac aacgagtcct gggcctcggg
      361 cccgtggaag acggtggagt tcttcagcta cttcaccatc ctcagcaacc tgctggccgg
      421 ggtggtgctg accgcgcccc tggtcgcgcc cgcctcgcgc ctggcggcct gggcggaggc
      481 gtcctcgacc cgcatcgcgg cggcgaccta tctggcgatc accgccctcg tctatcacgg
      541 gctgctggcc gacatctggg accccaaggg cctgggcctg gtggccgaca ccctgctgca
      601 caccgtgacg ccgatcgccg tcctggccga ctggctgctg cgcggcggaa aaggcccggc
      661 ccgctggagc ggcgcgttca aggccctggc cttcccggcc ctgtttggcg tctggacgct
      721 gctgcacggc gccctgaccg atttctatcc gtatccgttc ctggacgtga ccaaacgggg
      781 ctatcccggc gttatggtga tgatgctggc gatgagcgcc ggcttcctgg tcgtggccgt
      841 ggccttcacg gtgctgcatc gcgcccgcgc caggctggcg atcgaccggc gcgcgcccct
      901 atccgcctga ccgtctttcc ggagttttcc atgtccaagc gcgttcccgc cgtccgttcg
      961 ggcgatgtcc tgatccccgc cctgggcttt ggcacctggc agctggacca tggaaccgcc
     1021 cagccgctgg tcgagaccgc gctggagatc ggctatcgcc acatcgacac cgcccaggcc
     1081 tatcgcaacg agcgcgacgt cggcgcggcc atcgccgcca gcgggatcag gcgcgacgac
     1141 atcttcctga ccaccaaggt gtggatcgac cagttcgccg ccggcgacct gcaacgctcg
     1201 gccgaaaaga gcctggaaaa gctgggcgtc gaccatgtcg acctgctgct gctgcactgg
     1261 cccaagcccg atgtgcccct ggccgagacg atggcggcgc tcaacgcggt cagggagcgc
     1321 ggcctgaccc gcgccatcgg cctgtcgaac ttcccctcgg ccctgatggc ccaggcccag
     1381 accctgtccg ccgcgccgat cgccaccaac caggtcgaat accaccccta cctgtcgcaa
     1441 aagaccctga tcgccgccgc ccacgccgcc ggaacctcga tcaccgcctg gtcgcccctg
     1501 gcccagggca aggtggccga agacccggtg ctgatcgcca tcggccaggc ccacggcaag
     1561 gcgccgggcc aggtgaccct gcgctggctg atccagcagg gcgtcatcgc catcccccgc
     1621 accaagaccc cggcccgcat cgccgagaac tttgacgtgc tggatttcgc gctgtccgac
     1681 gacgagatgg cggcggtgtt tgccctggcg cggccggacg ggcg
//
LOCUS       DAOMEO010000766         1723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7123_length_1723_cov_2.556954, whole genome shotgun sequence.
ACCESSION   DAOMEO010000766 DAOMEO010000000
VERSION     DAOMEO010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7123_length_1723_cov_2.556954"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PLH31_15115"
     CDS             <1..165
                     /locus_tag="PLH31_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HQR90557.1"
                     /translation="HGLTPNLPDDMKTDYVQPGVGHYGVFNGRRFREEIYPRVRSFIR
                     KSDPNFGEEI"
     gene            223..939
                     /locus_tag="PLH31_15120"
     CDS             223..939
                     /locus_tag="PLH31_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQR90558.1"
                     /translation="MFTAAQRFSDGDRLDVAGCPVRLKVDGRARRISLRVDRARSEVV
                     ALAPTPRRLAEAVAFAHEKSAWIAGQLAALPGRISIAPGGVLRLYDRAYQLEVGPGRA
                     RLDHDRIIAPGDANWGLKVLRLAKREALTVLTERTAVHAAALGRPMPSVAVADPKARW
                     GSCRPARPGTPAAIRYSWRLILAPAAVADYVAAHECAHLIEANHGPKFWALCERLAGD
                     PAPHRAWLRAHAAELHAISA"
     gene            1129..1593
                     /locus_tag="PLH31_15125"
     CDS             1129..1593
                     /locus_tag="PLH31_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90559.1"
                     /translation="MSVALLAALQIVTATSEWVWADISISDARGSVDRIQILPKVTIE
                     GDRQVLRTVVARRTNTFAGKTMEIQWSDTSRCPGLRASLDAISEVRVLAESTLSKSYE
                     GLTNPPPPPPHRPVATVEVAGMRMNAIPESPLGLWVHGVTVATAGCWSEKAP"
BASE COUNT          277 a          595 c          576 g          275 t
ORIGIN      
        1 cacggcctga cccccaacct gcccgacgac atgaagaccg actatgtcca gccgggcgtc
       61 ggccactatg gcgtcttcaa cggccgccgt ttccgcgagg agatctatcc ccgcgtccgg
      121 tcgttcatcc gcaagagcga ccccaacttc ggcgaagaga tctaggccgc cgtcccggcg
      181 cttgcgccgg cgcggcccag acgcgatgta gggaccatgg gtctgttcac cgctgcgcag
      241 cgcttctccg acggcgaccg gctggatgtg gccggctgcc ccgtgcggct gaaggtcgac
      301 ggccgcgcgc ggcgcatctc gctgcgggtc gatcgcgccc ggtccgaggt cgtggccctg
      361 gcccccaccc cgcgccgcct ggccgaagcc gtcgccttcg cccacgaaaa gtccgcctgg
      421 atcgccggtc agctggccgc cctgcccggc cggatcagca tcgcgccggg cggggttctg
      481 cgcctgtacg acagggccta tcagctcgag gttggtccgg gccgggcccg cctcgaccac
      541 gaccgcatca tcgcgcccgg cgacgccaac tggggcctga aggtgctgcg tctggccaag
      601 cgcgaggcct tgaccgtgct caccgagcga accgccgtcc acgccgccgc cctgggccga
      661 ccgatgccca gcgtcgcggt ggccgatccc aaggcgcgct ggggctcctg ccgcccggcc
      721 cgtcccggaa ccccggcggc gatccgctac agctggcgcc tgatcctggc cccagcggcg
      781 gtggccgact atgtcgccgc ccacgaatgc gcccacctga tcgaagccaa tcacggtccg
      841 aagttctggg cgctgtgcga gcggctggcg ggggatcccg cgccgcaccg ggcctggcta
      901 agggcgcatg cggcggagtt gcacgcgatc tcggcgtgag aggacgggcg cgcaccggaa
      961 ggggcaaccc cggtcgctgg cgctgaaagc ggacatgcca atgggctgct gagggtggag
     1021 ggcggacgca acgaaccacg cgcgcaaagg ttggaatgtg gatgccgccg ccgcgtccct
     1081 tgctcaggct ggtgggctgg aattgtctag cgcctaggag tgtgccgaat gtccgtagcc
     1141 ttgcttgctg cacttcagat cgtgactgcc actagcgaat gggtctgggc cgacatttcc
     1201 attagcgacg cgcggggttc tgttgaccgg atccagatcc tgccgaaggt gaccattgaa
     1261 ggcgaccggc aagttctgcg gactgtcgta gcgcgccgga cgaatacatt cgccgggaaa
     1321 acgatggaaa ttcaatggag cgacaccagc cgttgccctg ggctacgcgc cagcctggat
     1381 gccatcagcg aagtgagagt tttggctgag agcacgctgt ccaagtcata cgaggggctg
     1441 acaaacccgc cgcctccacc gccccacagg ccggttgcca ccgtggaggt ggcaggaatg
     1501 agaatgaacg ctatcccgga aagcccattg gggttgtggg tgcacggcgt caccgtggcg
     1561 acggccggat gctggagcga gaaggccccc tagttgttgg ctcgcccaga tcctctatcg
     1621 ggctttccca acttccgctc agggtcgaaa gcacgccttg ccttccctcc cacaagcgga
     1681 cgccccgcgt cccgctcatc cttaaatctc ttggaggctg cag
//
LOCUS       DAOMEO010000767         1719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7137_length_1719_cov_2.254808, whole genome shotgun sequence.
ACCESSION   DAOMEO010000767 DAOMEO010000000
VERSION     DAOMEO010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7137_length_1719_cov_2.254808"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLH31_15130"
     CDS             complement(<1..497)
                     /locus_tag="PLH31_15130"
                     /inference="COORDINATES: protein motif:HMM:NF018960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR90560.1"
                     /translation="MGRAAQHQASQQGAKAPRQPAHRSFRRLHGDPLHTTSSNRSASK
                     NRAVTRLPGRLAPKRDDRGSGPSLSASLVRPKTAVEGPAEGTTMFHPSTQRLIDYWRD
                     RKGDAPAPSRSDINPGDFTDLLPQVFIVGREKLGRMPFRLTGGFLTDLHARDLRGEDL
                     LSLWAL"
     gene            515..1225
                     /locus_tag="PLH31_15135"
     CDS             515..1225
                     /locus_tag="PLH31_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90561.1"
                     /translation="MPAAAAGVLQAAAGSAQTAQAAQQHAKNQVSAQDGGATTNVYYG
                     GGSHGGYYSGPMSTGYIQGLNVEGGEMRRRASYEATRTKLRRVVIRAVCLDDRDVPHP
                     AAQVTPDRDIDNRYDGELYRCIAGSRMQYVIGDYEGAIDISTNGRGAQTFICAKNEAL
                     YHTPGAGQAGTLSCRPARPARDCNERSLLRRFGAGVKILTLLTSETYTAYREEQVRET
                     SASSMSFSLDGGVGGVVY"
     gene            complement(1290..>1719)
                     /locus_tag="PLH31_15140"
     CDS             complement(1290..>1719)
                     /locus_tag="PLH31_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQR90562.1"
                     /translation="LTDELREKQGATYSPSAGATHSVVFTDWGYLSVSVEVPPEQLDA
                     VAATIRQIAADLRDKAVTADELERAKKPRIDQIEKARVTNEYWLGNLSGAQSDPRILD
                     ATRSVVAGLERVTASDVQKAARTFLGDDKSWTLLVKPEGK"
BASE COUNT          275 a          571 c          572 g          301 t
ORIGIN      
        1 agggcccaca ggctgagcag gtcctcgccg cgcaggtcgc gggcgtgcag gtcggtcaga
       61 aaacccccgg tcagacggaa gggcatccgg cccagtttct cgcgtccgac gatgaaaacc
      121 tgcggcagaa ggtcggtgaa atcgcccgga ttgatgtcgc tccgcgatgg cgcgggcgcg
      181 tcgcctttgc ggtcgcgcca atagtctatc aaccgttgtg tgcttggatg gaacatcgtc
      241 gtcccctcgg cgggtccttc aacggctgtt ttcggccgca caaggcttgc tgagagactc
      301 gggccagagc cccgatcgtc ccgttttggc gcaagcctgc cgggaagacg ggtgacggcc
      361 cgattcttgc tggccgagcg gttggacgac gtcgtgtgaa ggggatcgcc atgaaggcgc
      421 cgaaacgatc gatgcgcagg ctggcgtggg gctttggcgc cctgctggga agcctgatgc
      481 tgggcggcgc ggcccatgcg aagtgcgtca atgcttgccc gccgccgccg ccggtgtgct
      541 gcaagccgcc gccggctccg cccaaaccgc ccaagccgcc caacaacacg ccaaaaatca
      601 ggttagcgcc caagatggag gcgcgaccac gaacgtctat tatggcggcg gcagtcatgg
      661 gggctactat tccggcccga tgtcgaccgg ctatatccag ggcctcaatg tcgagggtgg
      721 cgagatgcgt cgccgcgcgt cctatgaagc cactcgcacc aagctgcgcc gcgtcgtcat
      781 ccgcgccgtc tgtctggacg atcgcgacgt gccgcacccg gccgctcagg tcacgcccga
      841 tcgcgacatc gacaatcgct atgacggcga gctgtatcgc tgtatcgccg gctcgcgcat
      901 gcaatatgtg atcggcgact atgagggcgc gatcgacatc agcaccaatg gtcgcggcgc
      961 ccagaccttc atctgcgcca agaacgaggc cctgtatcac acgcccggcg ctggccaggc
     1021 cggcacgctg agctgccgtc cggcccgccc ggctcgcgac tgcaacgagc gctctctgct
     1081 ccgccgcttc ggcgcgggcg tgaagatcct caccctcctg accagcgaga cctacacggc
     1141 ctatcgcgaa gagcaggtcc gcgagaccag cgcgtcgtcg atgagcttca gcctcgatgg
     1201 cggcgtgggc ggcgtcgtct actaggaccc gctttccttg tctgaacgtt caggccctct
     1261 cgcccggcgg cgagagggcc ttttcgtatc tacttgcctt caggcttgac cagcaaagtc
     1321 cacgacttgt cgtcgcccag gaaggtgcgg gcggccttct ggacatcgga ggccgtgacc
     1381 cgttccaggc cggcgacgac cgagcgggtg gcgtccagaa tgcggggatc gctctgggcg
     1441 cccgacaggt tgcccagcca gtattcattg gtcacgcggg ccttttcgat ctggtcgatg
     1501 cggggcttct tggcccgctc cagctcgtcg gcggtgaccg ccttgtcgcg caggtcggcg
     1561 gcgatctggc ggatcgtggc ggccacggcg tccagctgct ccggcggcac ctcgacactg
     1621 accgacagat agccccagtc ggtgaagacc acgctgtggg tggccccggc cgagggcgaa
     1681 taggtggccc cctgcttttc gcgcagttcg tcggtgagc
//
LOCUS       DAOMEO010000768         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7153_length_1717_cov_2.443442, whole genome shotgun sequence.
ACCESSION   DAOMEO010000768 DAOMEO010000000
VERSION     DAOMEO010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7153_length_1717_cov_2.443442"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="PLH31_15145"
     CDS             <1..>1032
                     /locus_tag="PLH31_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HQR90563.1"
                     /translation="AVVLRLAFPPRAYAKHGTSVETGLLVVDRGQAGETAPVIAAETL
                     ADAARAAGAVAPRPSAQPRQFRAVAGVAILAPRARALATPSNRLAFLATTAPLAYETK
                     AWSGEGHDVGLYQAYRLGRIGFPEDRPHPSPLVESGPMASVAPPAPTYRPILPAKITD
                     QGLVSDAQLETVIYAGEAHATLLPGRWRLGDAPHQVILVGEDTPDAVSFRRGFFLGDG
                     TGCGKGRQIAGVIADNLSQGRTRAVWLSKNDALLEDARRDWTAIGGEASDLTPQSAWK
                     QADAIRLDRGILFTTYATLRQPARGDRASRLDQIVAWLGADFDGVIVFDEAHAMANAA
                     GGAKGARGP"
     assembly_gap    1033..1042
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1043..>1717
                     /locus_tag="PLH31_15150"
     CDS             <1043..>1717
                     /locus_tag="PLH31_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HQR90564.1"
                     /translation="AGLALQNRLPDARILYVSATGATTPENLAYAARLGLWGGPDAPF
                     PTREAFMDAVETGGVAVMELIARELKAMGLYIARSLSFDGVEYNALRHPLSADDTGIW
                     DAWADAYQLIHHNLRAALEAVGVTEDGKPKSGQAASAVMSAFEGSKLRFFGHLLAGLK
                     APSLVASIRNDLAAGRSAIVQVVSTNEAVMERRLAEIPPEEWNNLAVDLTPKDQVLDY
                     LMGAFP"
BASE COUNT          239 a          613 c          593 g          262 t
ORIGIN      
        1 gcgcggtcgt cctgcgtctg gccttcccgc cccgcgccta cgccaagcac ggaacttccg
       61 tcgagaccgg actgctggtt gtggaccggg gccaggctgg cgagaccgca ccggtgatcg
      121 cggccgagac cttggccgat gcggcccggg ctgcgggcgc cgtggcgccg cgcccgagcg
      181 cccagccccg gcagttccgg gccgtcgcag gcgtcgccat cctggcgccg cgggcccgcg
      241 ccttggcgac cccatcgaac cgcctggcct ttttggcgac gacggcaccc ctcgcttacg
      301 agaccaaggc gtggtcgggc gagggacacg atgtcggcct ctaccaagcc taccggctgg
      361 gccgcatcgg cttcccggag gaccgtcccc acccctcccc cctcgtggaa tctggcccca
      421 tggcctcggt cgcgccgccg gcgccgacct accggccgat cttgcccgcc aagatcacag
      481 accagggtct ggtctcggac gcgcagctgg agacggtgat ctatgccggc gaggcccacg
      541 ccaccctgct gcccggccgc tggcggctcg gtgacgcgcc gcaccaggtg atcctggtgg
      601 gagaggacac cccggacgcg gtcagtttcc ggcgcggttt cttccttggc gacggcaccg
      661 gctgcggcaa gggccgccag attgccggcg tcatcgccga caacctttcg caagggcgga
      721 cccgcgcggt gtggctgtcc aaaaacgacg ctctgttgga ggacgcccgt cgcgactgga
      781 ctgccatcgg cggcgaggcc agcgacctga ccccgcaatc ggcctggaag caggccgacg
      841 ccattcgcct tgatcgcgga atcctgttca ccacctacgc caccttgcgc cagccggcgc
      901 gcggggaccg ggcgtcgcgg ctcgatcaga tcgtcgcttg gctgggggcg gacttcgacg
      961 gcgtcatcgt cttcgacgag gctcacgcca tggccaacgc cgctggcggc gcgaaaggcg
     1021 cgcgggggcc cannnnnnnn nnggctggcc tggccctgca gaaccggctg cccgacgccc
     1081 gcatcctcta tgtctcggcg accggcgcca cgacgccgga gaacctggcc tatgccgcgc
     1141 ggctgggcct gtggggcggc ccggatgcgc cgttcccgac ccgggaggcg ttcatggacg
     1201 ctgtcgagac cggcggcgtg gccgtgatgg agttgatcgc ccgggagctg aaggcgatgg
     1261 gtctgtacat cgctcgctcg ctgtcgttcg atggcgtgga gtacaacgcc ctgcgtcatc
     1321 cactgagcgc cgacgacacc ggcatctggg acgcttgggc cgacgcctac cagctgatcc
     1381 accacaatct gcgcgccgcg ctggaggcgg ttggggtcac cgaggacggc aagcccaaga
     1441 gcggccaggc ggcgtcggcc gtgatgtcgg cctttgaggg ctccaaactg cgcttcttcg
     1501 gccatctgct ggccggcctg aaggcgccgt ccctcgtggc ttcgatccgc aacgacctgg
     1561 cggccggtcg ctcggccatc gtccaggtgg tttcgaccaa cgaggcggtc atggagcggc
     1621 ggctggccga gatcccgccg gaggagtgga acaacctcgc cgtcgatctg acgccaaagg
     1681 atcaagtgct cgactatctg atgggggcct tcccgat
//
LOCUS       DAOMEO010000769         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7158_length_1716_cov_2.560506, whole genome shotgun sequence.
ACCESSION   DAOMEO010000769 DAOMEO010000000
VERSION     DAOMEO010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7158_length_1716_cov_2.560506"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="PLH31_15155"
     CDS             complement(<1..123)
                     /locus_tag="PLH31_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HQR90565.1"
                     /translation="MSIAFIFPGQGSQSVGMGADLAQAFAAAREVFQEVDDALGQ"
     gene            290..823
                     /locus_tag="PLH31_15160"
     CDS             290..823
                     /locus_tag="PLH31_15160"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR90566.1"
                     /translation="MNRLTAGLAAVAVLAASPAFAALKPGAKAPDFTAPAALAGKDYS
                     FTLSKALKKGPVVLYFFPAAYTAGCTAEAHEFAEATPDFEKLGATVIGVTAGNVDKIK
                     AFSKEHCRDKFAVAAAPAGLVKAYDVALPMPGGWSNRTSYVIAPDGKILMAYTDGNFA
                     GHVSKTMDAVKAYKAQK"
     gene            891..>1716
                     /locus_tag="PLH31_15165"
     CDS             891..>1716
                     /locus_tag="PLH31_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR90567.1"
                     /translation="MADLSQLEAVIRGVAIGGFAAAGLALAATPRPNPTRWLGATFFV
                     CAIAHVICGWRPELERFDPVWAASVVGTGLFWMFALDLLEDTPHPPAWRLVAPIGAFL
                     FWLAAQIAPPAWCPPIWGVYLALQILLVGHLLVVAWRGWRGDLVPQRRRLRPLLAASA
                     CGYVLVVAAKDMGWLPFLPTGTMGVIQASALAILALGTTLALLRAEPQLLEAQADASA
                     APTPEPSSMDLGPADRLVLARLDKAMTDNEVWRGEDLSIGALAALVGAPEHRLRKLI"
BASE COUNT          255 a          599 c          563 g          299 t
ORIGIN      
        1 ctggcccagg gcgtcgtcga cttcctggaa gacctcgcga gcggcggcga aggcctgcgc
       61 aagatcggcg cccatgccga ccgactggct gccctgtccg gggaaaatga aggcgatgct
      121 cattggaagc tcgctccgtt ctagagattc aatgccgtac ttggggccgg agggttaggg
      181 gaaagccccc ggacgggcaa gagcgacggc gcttgacttg cattccgcga gggtgcgtcc
      241 aaattgccaa ctgacccgta actcgggcac gaaaccgatg gagcttctca tgaaccgcct
      301 gaccgccggc ctcgcggccg tcgccgtgct cgccgcctca cccgccttcg ccgccctcaa
      361 gccgggcgcc aaggcccccg acttcactgc tcccgccgcc ctggctggca aggactacag
      421 cttcaccctg tccaaggcgc tgaagaaggg gccggttgtg ctgtacttct tcccggccgc
      481 ctacaccgcc ggctgcaccg ccgaggcgca cgaattcgcc gaggcgacgc ctgacttcga
      541 aaagctgggc gcgacggtga tcggcgtgac cgccggcaat gtcgacaaga tcaaggcctt
      601 ctccaaggag cactgccgcg acaagttcgc ggtggccgcg gcgcccgcag gtctggtcaa
      661 ggcctatgac gtcgccctgc cgatgccggg cggctggtcc aaccgcacct cctatgtgat
      721 cgcgcccgat ggcaagatcc tgatggccta taccgacggc aacttcgccg gccatgtcag
      781 caagacgatg gacgccgtca aggcctacaa agcccagaag tagcgaagct tttccggttt
      841 cggccagcct tggggcttga gcatcccgag gctggcgggg attgtcggtc atggccgatc
      901 tatcgcaact ggaagccgtc attcggggcg tcgccatcgg cggctttgcc gccgccggcc
      961 tggccctggc ggccacgccg cgcccgaacc caacccgctg gctgggcgcg acattctttg
     1021 tctgcgccat cgcccacgtc atctgcggct ggcgacccga actggagaga ttcgatccgg
     1081 tctgggcggc ctctgtcgtc gggacagggc tcttctggat gttcgccctc gacctgctgg
     1141 aagacacgcc gcacccgccg gcctggcgcc tggtcgcgcc gatcggcgcg tttctgttct
     1201 ggctagccgc ccagatcgcg ccgcctgcct ggtgtccgcc gatctggggc gtctatcttg
     1261 ctctccagat cctgctggtc gggcatctgc tggtggtggc ctggcgcggt tggcggggtg
     1321 acctggtgcc tcaacggcgg cgcctgcgac cgctcctggc cgcctcggcg tgtggctatg
     1381 tgctggtcgt tgcggccaag gacatggggt ggctaccgtt tctgccgacc ggcacgatgg
     1441 gcgtcatcca ggccagcgcg cttgcgatcc tggcgctcgg cacgacactg gcgctgctgc
     1501 gcgccgagcc acagctcctc gaggcccaag ccgacgcttc ggccgcgccc acccccgaac
     1561 cgtcgtcgat ggatctgggc cccgccgacc gcctggtgct ggcccgactg gacaaggcca
     1621 tgaccgacaa tgaggtctgg cgcggtgagg atctgtccat aggcgccctg gcggccctgg
     1681 tcggcgctcc cgagcatcgc ctgcgcaagc tgatca
//
LOCUS       DAOMEO010000770         1716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7160_length_1716_cov_2.370259, whole genome shotgun sequence.
ACCESSION   DAOMEO010000770 DAOMEO010000000
VERSION     DAOMEO010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_1716_cov_2.370259"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLH31_15170"
     CDS             complement(<1..561)
                     /locus_tag="PLH31_15170"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516983.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQR90568.1"
                     /translation="MTSVASSASNQGAFDKRDNVVIFDTTMRDGEQSPGASMSHDEKL
                     ELAKILEEMGVDVIEAGFPIASNGDFEAVREIAKIVKNSTIAGLCRAHQVDIDRCAEA
                     LKGTQRGRIHVVIATSDLHMKHKLQMEPDAVIDVIARSVTHARNLRDDVEWSAEDATR
                     SDRQFLRRAIETAIKAGATTINLPDTV"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<858..>1716)
                     /locus_tag="PLH31_15175"
     CDS             complement(<858..>1716)
                     /locus_tag="PLH31_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HQR90569.1"
                     /translation="HGWPRFGKETVRTVLEQHRDTYKYLHDQSVRLLNQGLTGDEIAA
                     QIQLPPSLSRDWFNRGYYGSMSFNSRAVYQRYMGWYDANPVHLAPEPPAETGKRYVAA
                     MGGADKVKALAAEAAKAGDYVWAAKLLNHVVMGDEGDRAARNQLAGVYEQLGYQTENA
                     IWRNMYLTATDELRGGVRKIPASMASLDMLQGLESQMIFDVLAIRLNAEKVGDERLKL
                     AFVFPERNERFLVEVRNRVLVAQPLVEGEKVDATLTVARPVFLDSLFRGASLLPKILT
                     GEVKLEGDRA"
BASE COUNT          284 a          541 c          554 g          327 t
ORIGIN      
        1 gacggtgtcg ggcaggttga tcgtcgtcgc gccggccttg atggccgtct cgatggcgcg
       61 gcgcaggaac tgacggtcgc tgcgggtggc gtcctcggct gaccactcga catcgtcacg
      121 caggttgcgg gcatgggtga cagagcgggc gatcacgtcg atgacggcgt ccggctccat
      181 ctgcagcttg tgcttcatgt gcagatcgct ggtggcgatc accacgtgga tgcggccgcg
      241 ctgcgtgccc ttcagggcct ccgcacatct atcgatatcg acctgatggg cgcggcacag
      301 gccggcgatg gtgctgttct tgacgatctt ggcgatctcg cggaccgcct caaagtcgcc
      361 gttcgaggcg atcgggaagc cggcctcgat gacatcgacc cccatctcct cgaggatctt
      421 ggccagttcc agcttttcgt catgcgacat ggacgcgccg ggcgactgct cgccgtcgcg
      481 catggtggtg tcgaagatca cgacattgtc gcgcttgtcg aaagcgccct ggttagaggc
      541 gcttgaggct acggaggtca tttgaatact cgtcggaagg tgtctgcgtg cggggcggct
      601 gcgggcaaat catcccctgc gcgcccggtc acgacgatgc gtgaccctag agcgcccagg
      661 ggctggtaag ccgaagcaga agcagggtgc gcgcgacgtt catgcgtact ttctacacat
      721 cgcgcccaat cccagcaagc attttgcgcc tggagcgcga ttggccgcat gcagcgcaac
      781 ttctatccaa gcatcagcta tttatcgcgc gtgacgatgg gaaaatcgtt ggggaaagcg
      841 tcaaaccnnn nnnnnnncgc ccgatctccc tccagcttga cctcgccggt caggatcttg
      901 ggtagcagcg acgcgccccg gaacagcgag tcgaggaaca ccggccgggc cacggtcagg
      961 gtggcgtcga ccttttcgcc ttcgaccagg ggctgggcga ccagcacccg gttgcggacc
     1021 tcgaccagga agcgctcgtt ccgttccggg aagacaaagg ccagtttgag ccgctcgtcg
     1081 ccgaccttct cggcgttcaa ccggatagcc aggacgtcga agatcatctg gctttccagg
     1141 ccctgcagca tgtcgagcga ggccatcgag gccgggatct tgcgcacgcc gccgcgcagc
     1201 tcgtcggtgg ccgtcagata catgttccgc cagatggcgt tctcggtctg gtagccgagc
     1261 tgttcataga cgccggccag ctggttccgg gcggccctgt cgccctcatc gcccatcacg
     1321 acgtgattga ggagcttggc ggcccagacg taatcgcccg ccttggcggc ctcggcggcc
     1381 agggctttga ccttgtcagc cccgcccatc gccgcgacat agcgcttgcc ggtctccgcc
     1441 ggcggctccg gggccagatg aaccggattg gcgtcgtacc agcccatata gcgctgatag
     1501 accgcgcggc tgttgaagct catcgagccg tagtagccgc gattgaacca gtccctggac
     1561 aggctcggcg gcagctggat ctgcgcggcg atctcatcgc cggtcaggcc ctgattgagc
     1621 aatctaaccg actgatcgtg cagatacttg taggtgtcac gatgctgctc cagcacggtc
     1681 ctgacggtct ccttgccaaa gcgcggccag ccgtgg
//
LOCUS       DAOMEO010000771         1715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7168_length_1715_cov_2.111446, whole genome shotgun sequence.
ACCESSION   DAOMEO010000771 DAOMEO010000000
VERSION     DAOMEO010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_1715_cov_2.111446"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLH31_15180"
     CDS             <1..661
                     /locus_tag="PLH31_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQR90570.1"
                     /translation="FFSANAAPGSFFEGMGPVTFDLSNPYVVVGLLFGGLLPFLFGGM
                     SMTAVGRAAEAVVAEVRRQFRENPGIMTYEVKPEYGKAVDILTKAAIREMIVPSLLPV
                     VSPIVLFFVINAIAGKVDAFASLGAMLMGVIVTGLFVAISMTSGGGAWDNAKKVIEEG
                     FTDKDGVLHTKGSEAHKAAVTGDTVGDPYKDTSGPAVNPMIKITNIVALLLLAVLAHG
                     V"
     gene            complement(740..1126)
                     /locus_tag="PLH31_15185"
     CDS             complement(740..1126)
                     /locus_tag="PLH31_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90571.1"
                     /translation="MKRLLLSLSALAIVSAAPLAAQAAPWQSVNQRQANLDRRIDQGV
                     RSGEITRSEARRLRDEFRSLGRLEVQYRRSGGGLSMQERRDLDLRFDRLSQRIYVQKH
                     DRQDRDRHDRDGRDGRHDGRHDGRHN"
     gene            1328..>1715
                     /locus_tag="PLH31_15190"
     CDS             1328..>1715
                     /locus_tag="PLH31_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQR90572.1"
                     /translation="MTLQQGLSFGLIGATVLCFIWGRWRYDLIALGALAAGIATGLIP
                     IEAAFSGFSNDIVIIIASALVLSAAIARSGLVDMLMAPLLPHLKDERSQVPVLAGVTT
                     ILSMATKNVGTLALMMPSALQIAKRSG"
BASE COUNT          282 a          528 c          571 g          334 t
ORIGIN      
        1 gttcttctcg gccaatgcgg cgccgggcag cttcttcgag ggcatgggtc cggtgacctt
       61 tgacctgtcc aatccctatg tggtggtcgg cctgctgttc ggggggctgc tgcccttcct
      121 gtttggcggc atgtcgatga ccgccgttgg gcgggccgcc gaggccgtcg tggccgaggt
      181 tcgtcgccag ttccgcgaga acccgggcat catgacctat gaggtcaagc ccgagtacgg
      241 caaggcggtc gacatcctga ccaaggccgc gatccgcgaa atgatcgtgc ccagcctgct
      301 gccggtcgtg tcgccgatcg tgctgttctt cgtgatcaac gccatcgccg gcaaggtcga
      361 cgcctttgct tcgctgggcg ccatgctgat gggcgtgatc gtcaccggcc tgttcgtcgc
      421 catctcgatg accagcggcg gcggcgcctg ggacaacgcc aagaaggtga tcgaggaagg
      481 cttcaccgac aaggacggcg tcctgcacac caagggttcg gaggctcaca aggccgccgt
      541 caccggcgac accgtcggcg acccctacaa ggacacctcg ggtcccgccg tgaacccgat
      601 gatcaagatc accaacatcg tggctctgct gctgctggcg gtcctggccc acggcgtctg
      661 atccagacgc gccgtagaaa cgacgacgcc ccggagagtg atctccgggg cgtttccatt
      721 tcagcctcgg ctgacaggct tagttatggc ggccatcgtg gcgaccgtca tgacggccat
      781 cgcgaccatc ccggtcgtgg cggtcacgat cctggcggtc gtgcttctgg acgtagatgc
      841 gctggctcag gcggtcgaag cgcaggtcca ggtcacggcg ttcctgcatc gacaggccgc
      901 cgccactgcg acggtactgg acttcaaggc gccccaggct gcggaactcg tcgcgcaggc
      961 ggcgggcttc cgagcgggtg atctcgccgc tgcggacgcc ctgatcgata cggcgatcca
     1021 gattggcctg acgctgattg acggactgcc agggggcggc ttgggcggcg aggggagcag
     1081 ctgaaacgat ggccaaggcg gagagcgaga ggaggaggcg cttcatggcg agaacctttt
     1141 ttcgaaactt gcgatcgctg gggagtgcga tctgatgagc ccatagacca ctcctcatct
     1201 gaacgttccc ggaccccctt gttatgttgg gttcatgagg accaccgggt gagtgaacgg
     1261 aacccctcca ggatcgttga gccccgaagc cgagcttctc ggcatctaga gttgacgggg
     1321 cgtacacgtg acattgcagc aagggctgtc cttcggcctg atcggcgcga cggtgctgtg
     1381 tttcatctgg gggcgttggc gctatgacct catcgcgctg ggggcgctgg cggcgggcat
     1441 tgccacgggg ctgattccga tcgaagctgc cttctcgggc ttttccaatg atatcgtcat
     1501 catcatcgcc agcgccctgg tgctgagcgc agccatcgcc cgatccgggc tggtcgacat
     1561 gttgatggcg ccgctactgc cccatttgaa ggacgagcgc tcccaggtgc cggtactggc
     1621 gggagtgacc accattcttt cgatggcgac caagaatgtc ggcaccctgg cgctgatgat
     1681 gccttcggct ctccagatag ccaagcgtag tggag
//
LOCUS       DAOMEO010000772         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7175_length_1713_cov_2.826900, whole genome shotgun sequence.
ACCESSION   DAOMEO010000772 DAOMEO010000000
VERSION     DAOMEO010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7175_length_1713_cov_2.826900"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLH31_15195"
     CDS             complement(<1..960)
                     /locus_tag="PLH31_15195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HQR90573.1"
                     /translation="MGNARTALAVSFAALLALGSSGAFAADWKLVEVAGVVRIAAPGK
                     APTSANANQVLPVGSIVTTAAGGRAALFNGAQRITLGPNSRMTVAPESTDGMTRVLQD
                     LGSILFQIDKKPSQHFSVETPLLAAIVKGTTFTVNVEVAADSVHVAEGLVEVRANEGG
                     ATRDVGAGATGAIARSEPGQLTVETPRASTEAPLPLQTAAIDYNVVSGGLVEGPTPTG
                     AAAPSESIRSVAETSSPTQAEGPMASPVAQTTMMAFAGNLATGGDAVASGLDAAPIPS
                     ALDAPVSAGGNDNAGGGNAGGNGNGAGGGNSNAGGGNDNAGGGN"
     gene            1248..>1713
                     /locus_tag="PLH31_15200"
     CDS             1248..>1713
                     /locus_tag="PLH31_15200"
                     /inference="COORDINATES: protein motif:HMM:NF020068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQR90574.1"
                     /translation="MLTGLTIDGVSAYPLKTLAPLYADYLAREVTIEDLVKIAQAITD
                     KYRADGYFLSRAIVPPQAPGGHARIRVYEGYIEEVEVTGDAAPALEAMLSGLTERRPL
                     RLTDLERRLTLAADLPGVQPRSRIEPILDDPARHKLVVTAGLKRWSGSLYMDN"
BASE COUNT          297 a          554 c          545 g          317 t
ORIGIN      
        1 gttgccgcca ccggcattgt cgttgccgcc gccagcgttg gagttgccgc cgcctgcgcc
       61 gttgccgtta ccgcccgcgt tgccgccacc ggcattgtcg ttgccgccgg cgctcaccgg
      121 cgcatctagc gcgctcggga tcggggcggc gtccaggccg gacgctacag cgtcgccgcc
      181 ggtcgcgaga ttacccgcga aggccatcat cgtcgtctgc gccacaggcg atgccatggg
      241 gccttccgcc tgggtcgggg aagacgtttc ggcaacgctc cgaatggact cggacggcgc
      301 cgccgctccg gtcggggtcg ggccctcgac cagcccacca gacacaacgt tgtaatcgat
      361 ggcggcggtt tgcaggggga gaggcgcttc tgtggacgcg cgtggcgtct caaccgtgag
      421 ctgcccgggc tcggaccttg cgattgcgcc cgtcgcgcca gcgccaacat cacgcgtcgc
      481 gccgccttca ttggcgcgaa cttcgaccag tccttcggcc acgtgcacgg agtcggcggc
      541 cacttccacg ttaacggtga acgtcgtgcc tttgacgata gccgcgagga gcggcgtttc
      601 gacgctaaag tgctgggaag gcttcttatc gatctggaag aggatggagc ccaggtcctg
      661 gaggacgcgg gtcatgccgt cagtcgattc aggggccacg gtcatgcgac tgtttggacc
      721 cagggtgatg cgctgggcgc cgttgaacaa ggccgctcgt ccgcctgccg ccgtcgtaac
      781 gatgctgccc acgggcagga cctggttggc gttggcggag gtgggggcct tcccaggcgc
      841 agcgatacgg accacaccgg cgacctcgac cagtttccaa tcagccgcga atgctccact
      901 ggaacccagg gcgaggagcg ccgcgaagga tactgctagc gcggtacgag cattgcccat
      961 gtggttgcct ctaaagtcta gtcggaccat gtaattttac cgacctctaa aacaatctga
     1021 ctaaagctcc gctaaggggc gtggtttgca aacttcgtta acgagcgatt tatatgtacc
     1081 gtcgatcatc agtatcgatg agcaacataa aaacatttgt taagagacgc tcgcagccca
     1141 gtattggagg ctgtctgctc gtcgcaacac tcgcagccac cagcttggcg tcacccgtcg
     1201 ctgcgcgacc acagccgtcg ccagatcgac ccgcggccgc gccagtggtg ctgacagggc
     1261 tgacgatcga cggtgtgagc gcttatccgc tcaagacgct ggccccgctc tatgccgact
     1321 atctggcccg cgaagtcacg atcgaggact tggtcaaaat cgcccaggcc atcaccgaca
     1381 agtatcgggc cgacggctat ttcctgtcgc gcgcgatcgt gcctccacag gcgccgggcg
     1441 gccatgcccg catccgggtt tatgaaggtt atatcgaaga ggtcgaagtc accggtgacg
     1501 ccgcgccggc ccttgaagcc atgctctctg gcctgaccga gcggcgcccc ctccggctga
     1561 ccgaccttga gcgcaggctg actctggcgg ccgacctgcc tggcgtgcag ccgcgttcac
     1621 gtatcgagcc gattctggat gatccggcga ggcacaagct tgtcgtcacc gcaggtctca
     1681 agcggtggag cggcagcctc tacatggata atc
//
LOCUS       DAOMEO010000773         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7178_length_1713_cov_2.268396, whole genome shotgun sequence.
ACCESSION   DAOMEO010000773 DAOMEO010000000
VERSION     DAOMEO010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_1713_cov_2.268396"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1559)
                     /locus_tag="PLH31_15205"
     CDS             complement(132..1559)
                     /locus_tag="PLH31_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQR90575.1"
                     /translation="MDFLRLDAHDQLEALNAGRISALDALNLAIARADAVGGTLNAVV
                     ARRLDLARDEARRIDEARSLGEPMGRLAGLPMTIKDSLDIEGLPASVGRASHLDRRSR
                     DADSVRRVRAEGAIVWGQTNTPIDAADWQTFNPLYGVTNNPWNLKRTPGGSSGGSAAA
                     LAAGISALEIGADIAGSLRIPAAFCGVFAHKPTFGAISQAGLAPPQTLDLDMAVVGPM
                     ARSARDLELLFSILSQRPFTPAPPKDLANVRIGLWLDEPGFELDPGMRRVIERFAKQL
                     QDHGAIVEPASGPVSGGETLEIYTGLLYPLLWAKAPAAEMALYEALRGPAKLARRLGA
                     GPLSWAQGVLAATARYRDWRATEERRAARIEAIDTFFTRFDVLLAPCAPTPAFEHDHG
                     PRPTRKLKLSTGRRIDYLQLMSWPALATVWGLPATAIPVGLTDDGLPVGVQLIGPRDG
                     DARTLGVARAIEERLGAFPAPPPLS"
     gene            1533..>1713
                     /locus_tag="PLH31_15210"
     CDS             1533..>1713
                     /locus_tag="PLH31_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90576.1"
                     /translation="MGVEPQEVHADVRCGEVRQSTLGQTALAAPEASASDHAATLRWA
                     GTSKTRVAPRHTSIRS"
BASE COUNT          267 a          570 c          617 g          259 t
ORIGIN      
        1 ccatggccgc tgtcgtcggc gacggcgttg gggcgagggg tgtcggtcat ggtcggaatc
       61 cctgatgcgt gcagccaagc tcgtgcgtcg atgcgccatg cgcaagggcg cagcgcgtcg
      121 atttcagtgc ttcaagacaa gggcggtggg gcagggaatg cgccaagccg ctcttcgatg
      181 gcgcgggcca cgcccaaggt ccgggcgtcg ccgtcacgcg ggccgatcag ctgaacgccg
      241 accggcaggc cgtcatcggt aagtccgacg ggaatggccg tggccggcag gccccagacc
      301 gtcgccaacg ctggccagct catcaactgg agatagtcga tccgccggcc ggtcgagagc
      361 ttcagtttcc gggtcgggcg cggaccgtgg tcgtgctcga aggccggcgt cggggcgcag
      421 ggcgccagca gcacgtcgaa ccgcgtgaaa aaggtgtcta tggcctcaat ccgagcggcg
      481 cggcgctctt ccgtcgcccg ccagtcgcga tagcgggccg tcgcggcgag tacgccctga
      541 gcccagctga gcgggccagc gccaagccgc cgcgccagct tagcggggcc tcgcaatgcc
      601 tcgtacagcg ccatttccgc cgctggagcc ttggcccaga gcagcgggta gagaagcccg
      661 gtatagatct caagcgtctc gccgccgctc accggaccgc tggcaggctc gacgatcgcg
      721 ccatggtctt ggagctgttt ggcgaagcgc tcgatcacgc gacgcatccc cggatcgagt
      781 tcgaagcccg gttcgtccag ccacaggccg atccgcacgt tcgccaggtc cttcggcggc
      841 gccggggtga aggggcgttg gctgaggatc gagaacagca gttccaggtc tctggccgaa
      901 cgggccatcg ggccgaccac ggccatgtcc aggtcgaggg tctgcggcgg cgccagtccg
      961 gcctgtgata tcgcaccgaa cgtcggcttg tgagcgaaga cgccgcagaa ggcggcgggg
     1021 atgcggagtg agccggcgat atcggcgccg atctccagcg ccgatatgcc cgccgccagg
     1081 gccgcagccg acccgcccga ggagccgccc ggcgtacgct tcaggttcca ggggttgttg
     1141 gtcacgccgt aaagcgggtt gaaggtctgc cagtccgcgg cgtcgatcgg cgtattggtc
     1201 tggccccaga cgatcgcccc ttcggcgcgg acgcgccgga cggaatcggc gtctcggctt
     1261 ctgcggtcaa ggtggctcgc ccgcccgacg gaggccggca ggccctcgat gtccaggctg
     1321 tccttgatgg tcatcgggag gccagccagg cgtcccatcg gctcgcctaa cgatcgagct
     1381 tcgtctatcc gacgggcttc atcgcgggcc agatccaggc ggcgcgccac cacggcgttc
     1441 agggttccgc caacggcgtc ggcgcgcgca atcgccaagt tcaaggcgtc cagcgccgag
     1501 atgcggccag cattcagcgc ctcgagctgg tcgtgggcgt cgagccgcag gaagtccatg
     1561 ccgatgtccg gtgcggtgaa gttcgccaga gtactctagg tcagacggcg ctggcggcgc
     1621 cagaggcgag cgccagcgat cacgcggcga cgctgcgttg ggcgggcacg tcgaagacgc
     1681 gcgtcgcgcc gcgccacacc tcgatcagat cgc
//
LOCUS       DAOMEO010000774         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7209_length_1707_cov_1.990315, whole genome shotgun sequence.
ACCESSION   DAOMEO010000774 DAOMEO010000000
VERSION     DAOMEO010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_1707_cov_1.990315"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..752)
                     /locus_tag="PLH31_15215"
                     /pseudo
     CDS             complement(1..752)
                     /locus_tag="PLH31_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            complement(824..1498)
                     /locus_tag="PLH31_15220"
     CDS             complement(824..1498)
                     /locus_tag="PLH31_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90577.1"
                     /translation="MRNGQLIVAAAIVVVTVGLGATITSRVTAQTRVDPTTGAPIGAA
                     RPPVATRPPAATTVPPRRGGRVDWQGAVGGLRQSGGASETLGANLDKAEVDATRVPIL
                     LPNDPKLTAGARIYSFGDYYTITADAPGAGISLSGTTAIVPLPASRPLKIEPAGPEGL
                     TVQRTVDGQLASFMRYGVLYTVEIRCEKAGDPRCADDNYVLGLVGKTTTVVLGAAARA
                     EAGLGG"
BASE COUNT          230 a          560 c          632 g          285 t
ORIGIN      
        1 ctcagatagg gcggtatgaa gctgctgatc gccaccggtt tgccgtcggt ggtcacgccc
       61 gccaggcccg cccgcatctc gaaatgcagg tggatggtgg tgggcgtgcc gccaaagtcg
      121 ttggacacct tgcccagctt ctggccggcg gtgaccttgg ttccctcggc caccgccagg
      181 gtcgacatct gcatgtgcag atagcggtag gtgtagtgcg gcgcggcctt gcccacgagg
      241 gtgacggtgt aggtcccgat atcggtgatg gtgccggctt cggtggcgac cgcccagtgc
      301 tcgttcttct tgcaggtggc cgggcggatg tcctggccct ggtggccggt tcccgagccg
      361 cacagcgggc tggtgcgcga gcgggcctcg cagaaattgt cccgccaggg caggctgtaa
      421 ttgaggctgt cgcactggct gggcttgccg ggtgggccca tgccgccgcc ggggccatag
      481 acctgggaat tggcgaagcc cgcctcctgt agcggccagc acatggccgg ggcccagacg
      541 gtgcggtcta gatagcccga gccctgaccg ctgggcagct ggccggcggg gccatgattg
      601 aaggtggcgg gcgagggggc gggcggctcg gccggctgca ccggatcgcc ggggctgggg
      661 ggcaggggct ccgaagcgtc ctgctcggcg ccaccatcga ccggcgtatc atcggcctgg
      721 ggcgggccgg gatcggccga gggcggcttg ggcggcgcgt ccggcggtca gggccagggc
      781 cagaacggcc agagccaggg gagggatcag cttgctcatc ggctcagccc cccaggccgg
      841 cttcggcgcg ggcggcggcg cccagcacaa cggtggtggt cttgcccacc aggcccagca
      901 cgtagttgtc gtcggcgcag cgcggatcgc ccgccttctc gcagcggatc tcgacggtgt
      961 agagcacccc gtagcgcatg aagctggcca gctggccatc caccgtgcgc tgcaccgtca
     1021 ggccctcggg gcccgcgggc tcgatcttca gcggcctgga ggcgggcagg ggcacgatgg
     1081 cggtcgtgcc gctcagcgag atgccggccc cgggcgcgtc ggcggtgatc gtgtaatagt
     1141 cgccaaagct gtagatgcgc gccccggcgg tcagtttggg atcattgggt agcaggatcg
     1201 gcacgcgggt ggcgtctacc tcggccttgt ccagattggc ccccagggtt tccgaagccc
     1261 cgccgctctg gcgcagcccg cccacggcgc cctgccagtc gacccggccg cctctgcggg
     1321 gcggtactgt agttgccgcg ggcggccggg tcgccactgg cgggcgcgcc gcgccgatcg
     1381 gggcgccggt cgtcgggtcc acgcgtgtct gggcggtgac gcgagacgtt atcgtcgcgc
     1441 caagccccac cgtcacgacg acaatggccg ccgcaactat cagctgtccg ttgcgcatgg
     1501 ccccaccaca taaccccgtt atctcaacct tagcgcggat tggcgaggct gttaaccctg
     1561 tcgactcgcc ctgcggcctg gggcggcgat tgaaccggcg cggtttcgtg gtctatatga
     1621 cccgtagttc aggcgccctg agtgtggcgg ccctgccaaa gcccagctta atcaaaggga
     1681 tgtaaggttc gccatgctgc tcaccat
//
LOCUS       DAOMEO010000775         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7214_length_1706_cov_2.264082, whole genome shotgun sequence.
ACCESSION   DAOMEO010000775 DAOMEO010000000
VERSION     DAOMEO010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7214_length_1706_cov_2.264082"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="dsbD"
                     /locus_tag="PLH31_15225"
     CDS             <1..1142
                     /gene="dsbD"
                     /locus_tag="PLH31_15225"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQR90578.1"
                     /translation="LSLAYVLAMASALGLLGVAAAWSGQNLQMALQSPLAIGAVSVLF
                     VVLALSMFGLFELQLPSAWVNAIGRLGQGNRGSLASAGLLGFTSALIVGPCVTAPLAG
                     ALIYIAQTGDVALGAASLFALGLGKGIPLIAFGTLGPKALPKAGAWMTVVRQAFGFVF
                     VATAIWMVSRIIPAEAGLALWALFAIGLAVWLGAFDTIGPDASGRRRAAKAAGIAAAL
                     YGIVLAVGAASGAGDPFRPLANLAAGTGGAPGSSEALAFRSAGTPAELQTEIAGRNGK
                     PSLLYVTADWCVTCAVIDRSVLSDPAVQSTLARFNLIKLDVSGNTPEQRQIMQGLQVV
                     GPPTMIFVDPEGREATGTRLVGDVTKATLQASVEQIGSDKTGTVR"
     gene            1139..>1361
                     /locus_tag="PLH31_15230"
     CDS             1139..>1361
                     /locus_tag="PLH31_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HQR90579.1"
                     /translation="MNAIAIGPFVFASEQFAAVLGIFTFTIATSILARRVDPIIGQWS
                     TWALIGGLVAARLGHVALHWQSFAAEPMRI"
     assembly_gap    1362..1371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1372..>1706
                     /locus_tag="PLH31_15235"
     CDS             <1372..>1706
                     /locus_tag="PLH31_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR90580.1"
                     /translation="IRSLKAGLAAAGALGLSLVLWVGVTQLTQATLGQPAPTIALQQM
                     DGPPLAVSDVIGKPAVVNLWASWCPPCRREMPLLAEVAASRDDVAFLFVNQGEGSETI
                     RAYLAKENL"
BASE COUNT          242 a          580 c          548 g          326 t
ORIGIN      
        1 ccctgtcgct tgcctacgtg ctggcgatgg caagcgcgtt gggcctgctc ggcgtggcgg
       61 cagcctggtc cggacagaat ttgcagatgg cgcttcagtc gccgctcgcc atcggcgcgg
      121 tctccgtgct cttcgtcgtg ctggccttgt cgatgttcgg gctgttcgaa ctgcaactgc
      181 catcggcctg ggtcaatgcc atcggccggc tcggtcaggg caatcgcggc tctttggcct
      241 cggccgggct gctcggcttc acctcggcac tgatcgtcgg gccttgcgtg accgcgccgc
      301 tcgccggtgc tctgatctac atcgcccaga ccggcgacgt cgcgctcggg gcagcatccc
      361 tcttcgcgct tggcctgggc aagggcattc cgctgatcgc tttcggcaca ctcggcccga
      421 aggcgctgcc caaagcggga gcgtggatga ccgtcgtccg gcaggcgttc gggttcgtct
      481 tcgtggcgac cgccatctgg atggtctcgc gcatcatccc ggcagaagcg ggccttgcgc
      541 tctgggccct attcgcgatc ggtcttgcgg tctggctggg cgccttcgat acgatcgggc
      601 cggatgcttc ggggcggcgg cgggcggcaa aggccgccgg catcgcggct gcactctatg
      661 gcatcgtgct ggccgtcggg gctgccagcg gtgccggaga tcccttccgg cctctcgcca
      721 accttgcggc cggaaccgga ggcgctcccg gttcttcgga agcgcttgcc ttccgcagcg
      781 cgggcacgcc cgccgagctt cagacggaaa tcgcgggacg gaacggtaaa ccgagcctgc
      841 tctacgtcac cgcggactgg tgcgtaacct gcgccgtcat agaccgcagc gtcctgtccg
      901 atcccgccgt ccagtccacg ctcgcccgct tcaatctcat caagctcgat gtcagcggca
      961 acacgccgga acagcggcag atcatgcagg gccttcaggt ggtcggtccg ccgacgatga
     1021 tcttcgtcga tcccgaaggg cgagaggcga ccggaacgcg gcttgtggga gatgtgacca
     1081 aagcgacctt gcaagcctct gttgagcaga ttgggtccga caagaccggg acggtgcgat
     1141 gaacgccatc gcgattgggc ctttcgtctt cgcctcggag caattcgcgg ccgttctcgg
     1201 catcttcacc ttcacgatcg cgacgtcgat cctcgcgcgc cgcgtcgacc ctatcatcgg
     1261 tcaatggtcg acctgggcgc tgatcggcgg cctggtcgct gcccgccttg gtcatgtcgc
     1321 gctgcattgg caaagcttcg cggccgagcc catgcggatc tnnnnnnnnn ntcatccgct
     1381 cgctcaaggc cgggcttgct gccgcagggg ccctcggcct cagcctcgtg ctgtgggtcg
     1441 gcgtgaccca actcacccag gccacgctcg gtcagcccgc cccgacaatc gcgcttcagc
     1501 aaatggacgg tcctccgctc gcggtcagcg acgtgatcgg caaacccgct gtcgtcaatc
     1561 tctgggcgag ctggtgcccg ccctgccgca gggaaatgcc gctgctggcg gaagtcgccg
     1621 caagccgtga tgacgtcgcc ttcctcttcg tcaatcaggg cgaaggctcg gaaacgatcc
     1681 gtgcctatct cgcaaaagaa aacctc
//
LOCUS       DAOMEO010000776         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7224_length_1705_cov_2.238788, whole genome shotgun sequence.
ACCESSION   DAOMEO010000776 DAOMEO010000000
VERSION     DAOMEO010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7224_length_1705_cov_2.238788"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLH31_15240"
     CDS             <1..708
                     /locus_tag="PLH31_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HQR90581.1"
                     /translation="ALARSQKVDLLQLSITRLAEQYLAFVQQARRVRFSLAADYLVMA
                     AWLAYLKSRLLLPKPERAKTEEPPAEEMAAQLAFRLAKLDVMRKAVEALKERPLLQRE
                     VFMRGDPEAVKIVSSTRLEGDLYGLMSAYIAQRKREHSRHYAPRPPTAYPLEDARDRL
                     RSLLPRLEDWTTLSTVAPVERYLEEDDGPSPASYMASTLAASLELVKEGVLEARQMEA
                     FSDVYLRNRAEPLEMVE"
     gene            705..1361
                     /gene="scpB"
                     /locus_tag="PLH31_15245"
     CDS             705..1361
                     /gene="scpB"
                     /locus_tag="PLH31_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQR90582.1"
                     /translation="MIDPLFTERCIEALLFAAAEPLSDIDLTKRLPEGCDIPAGIAAL
                     RARYEGRGIELVCVANRWRFQTAQDLAFLMTQEREEPRRLSKAAQETLAIIAYHQPVT
                     RAEIEAVRGVQASRGTIDVLLELGLVRMRGRRRSPGRPVTFGTTDAFLEHYGLATLSD
                     LPGVTEMKAAGLLEMNLPPGFSVPDPLGLRPGEDEDPLEDGDEAPEFAQDFLGEMEKG
                     "
BASE COUNT          272 a          565 c          589 g          279 t
ORIGIN      
        1 gccctggcgc gcagccagaa ggttgatctg ctgcagctgt cgatcaccag gctggccgag
       61 cagtatctgg cgtttgtcca gcaggcccgg cgggtgcggt tctcgctggc cgccgactat
      121 ctggtgatgg ccgcctggct ggcctatctg aaatcgcgcc tgctgctgcc caagcccgaa
      181 cgcgccaaga ccgaggagcc gccggccgag gagatggccg cccagctggc cttccgtctg
      241 gccaagctgg acgtgatgcg caaggccgtc gaagcgctga aggagcgtcc gctgctgcag
      301 cgcgaggtgt tcatgcgcgg cgatccggaa gcggtgaaga tcgtctcgtc gacgcggctg
      361 gagggtgatc tgtatggcct gatgagcgcc tatatcgccc agcgcaaacg cgagcacagc
      421 cgccactatg cgccgcgccc gcccacggcc tatccgctgg aagacgcccg cgaccgtcta
      481 cgctctctgc tgccgcgcct ggaggactgg accacactgt cgaccgtggc gcccgttgaa
      541 cgctatctgg aggaagacga cggcccgtcg ccggcgtcct atatggcctc taccctggcc
      601 gcgtcgttgg agctggtgaa agagggcgtg ctggaagccc ggcagatgga ggcgttctcg
      661 gacgtttatt tgcgcaaccg ggccgaaccg ctggagatgg tggagtgatc gaccccctgt
      721 tcaccgagcg ctgcatcgag gcgttgctgt tcgccgccgc cgagccgctc agcgatatcg
      781 acctgaccaa gcgcctgcct gagggctgcg atattccggc cggcatcgcc gccctgcggg
      841 cgcggtatga gggcaggggg atcgagctgg tctgcgtggc caaccgctgg cgcttccaga
      901 ccgcccaaga cctggccttc ctgatgacgc aggagcgcga ggagccgcgc cgcctgtcca
      961 aggccgccca ggagaccctg gcgatcatcg cctatcacca gcccgtcacc cgggccgaga
     1021 tcgaggccgt gcgcggggtc caggccagcc gggggacgat cgacgtgttg ctggagctgg
     1081 gtctggttcg gatgcgcggt cgccgtcgct cgccgggtcg gcccgtcacc ttcggcacca
     1141 ccgacgcctt cctcgagcac tatggcctgg cgaccctgtc ggacctgccg ggcgtcaccg
     1201 aaatgaaggc cgccggcctg ctggagatga acctgccgcc gggcttctcc gtgcccgacc
     1261 ccctgggcct gcgtcctggc gaggacgagg atccgcttga ggacggcgac gaggcgcccg
     1321 aatttgctca ggacttcctg ggggagatgg agaaaggatg aaggtagagg aggggcgcct
     1381 cctccctact ttccgtcatt cccgccacaa gggcgggagt tcaccagagc cgacaatccc
     1441 tggcggtggc gatctgaagg actgcttgcc ggagggtgtt agtgacgaat ttcgaacccg
     1501 aaccgccaag agggctgtct gttctggcgt ggcgactgtt tgactttagg cgaggcccat
     1561 agcgaggccg ccccttttgc ggatgtcggt aggccgaggc gtctaatccc ccccggccgc
     1621 gaggaacaac gctggcagca gggccagagc gacgccgatc ccggccaagg ctggccagcg
     1681 ccaggcgccc atgccgcgcc acgcc
//
LOCUS       DAOMEO010000777         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7227_length_1705_cov_1.821212, whole genome shotgun sequence.
ACCESSION   DAOMEO010000777 DAOMEO010000000
VERSION     DAOMEO010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_1705_cov_1.821212"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLH31_15250"
     CDS             complement(<1..351)
                     /locus_tag="PLH31_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR90583.1"
                     /translation="MSSRLAFMLSSALVSLALAGPALARQAEPTSVDAVVVTGSQVRL
                     PPPYAGGQVAEGARVGLLGALDTIDAPFSVTSYTEALIRNQQARSVADVLQNDPTVRV
                     TKGFGNFQELYVIRG"
     gene            complement(602..>1705)
                     /locus_tag="PLH31_15255"
     CDS             complement(602..>1705)
                     /locus_tag="PLH31_15255"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQR90584.1"
                     /translation="DCAGSRDKSVRTLFGRAEAAGLSTGVVSTARITHATPAAAYAHS
                     ANRDWENDSELPAKAKAEGCIDIARQMIEWPAGNGFEVMLGGGRAQFLPADQADVEDA
                     SAKGLRRDGRDLVSDWRKANPKGAYVWNAKGFDAVDPAEVTKLLGLFQRDHMTYEGER
                     AADPGGERSLAEMTAKAIAMLQRSPQGYVLMVEAGRVDHGHHEGLAGRALEEAIALDA
                     AVKRALAMVNLDETLVVVTADHSHGLTISGYPKRGNPILGTVQGQSGKVAKAPDGKAY
                     TTLSYATGPGGVDGPRADPATIDTTAPDYRQPALVLSRSAAHSGEDIALRAIGPQAHL
                     LQGTIEQNLIFHVMAHALGGQVAREAQVAGAID"
BASE COUNT          268 a          549 c          584 g          304 t
ORIGIN      
        1 gccgcgtatg acatacagct cctggaagtt gccgaacccc ttggtgacgc gcacggttgg
       61 atcgttctga agcacgtcgg cgacactgcg cgcctgctgg ttgcggatca gcgcctcggt
      121 gtaactggtg acggagaagg gcgcgtctat ggtgtcgagc gcgcccaaca ggccgacgcg
      181 cgcgccctcg gcgacctgac cgccggcata ggggggcggc aggcggacct gggatcctgt
      241 gacaacgacc gcgtcaacac tggtcggctc ggcttgccgg gcgagagcag ggcccgccag
      301 cgcgaggctc acgagagcag acgacagcat aaaagcgaga cgcgacgaca tggggatgtc
      361 ctgtattaag agtgcgtcgc aatagcgttg attgggtgaa gctacaacag ttcctttccc
      421 gtcaccgaaa atagggggct aatggctgga gccagcgctc catgccgtcg ctctccgccc
      481 atcaaggggc ctcccgtccc gagcgcccgc agtggacgct tggggcagta tccaggttgg
      541 gtgggcccga ccgtttcggc agctcaggca aagtccccag atcgggttgg ccttcgtcag
      601 ctcaatcgat ggcgccggcc acctgcgcct cgcgggccac ctgtcccccc agggcgtggg
      661 ccatgacgtg gaaaatcagg ttctgttcga tcgtgccctg aagaaggtgc gcctgtgggc
      721 cgatggctcg cagggcgatg tcctcacccg agtgcgccgc tgaacgcgag agaaccaggg
      781 caggttggcg atagtctggc gcggtcgtgt cgatcgtggc gggatcggcg cgcgggccgt
      841 caacgccgcc agggccggtg gcatagctca gggtcgtgta ggccttgccg tccggcgcct
      901 tggcgacctt gccgctctgc ccctggaccg tcccgagaat gggattgccg cgcttgggat
      961 agccagagat cgtcagaccg tggctatggt cggcggtcac cacgaccaag gtctcgtcga
     1021 gattgaccat ggccagggcc cgcttgacgg cggcgtccag cgcaatggcc tcttccaggg
     1081 cccggcccgc caacccttcg tggtgcccat gatcgacccg tccggcctcg accatcaaga
     1141 catagccttg cgggctgcgc tgcagcatcg cgatggcctt tgcggtcatt tccgccaggg
     1201 accgctcgcc gcccggatcg gcggcgcgtt cgccctcata ggtcatgtgg tcgcgctgaa
     1261 acaggcccag cagcttggtg acttcggcgg gatcgacggc gtcgaaaccc ttggcgttcc
     1321 agacataggc gccttttgga ttggccttgc gccagtccga gaccaggtcg cgtccatcgc
     1381 ggcgcaggcc cttggcgctg gcgtcttcga cgtcggcctg atcggcgggc aggaactggg
     1441 cgcggccgcc gccgagcatg acctcaaagc cgtttccggc cggccattcg atcatctgcc
     1501 gtgcgatatc gatgcagccc tcggccttgg ctttcgcggg aagttcgctg tcgttttccc
     1561 agtcgcgatt ggccgaatgg gcataggctg cagccggcgt ggcgtgggtg atccgggcgg
     1621 ttgagaccac gccggtggac aggcccgccg cctcggccct cccgaacagg gtccggacac
     1681 ttttgtccct ggagccagcg cagtc
//
LOCUS       DAOMEO010000778         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7238_length_1702_cov_2.740134, whole genome shotgun sequence.
ACCESSION   DAOMEO010000778 DAOMEO010000000
VERSION     DAOMEO010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7238_length_1702_cov_2.740134"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLH31_15260"
     CDS             complement(<1..410)
                     /locus_tag="PLH31_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HQR90585.1"
                     /translation="MPVDLPPAAVAPVVVSAARLPPLRGEAAFSVVRLDEAELAGAVR
                     IDEALSRTPGVSLFRRTSSLSANPTTQGISLRAIAPSGAGRTLVLLDGAPLNDPFGGW
                     VIWSQLAPEGVAGADIVRGAGAGPYGAGALTGVIS"
     assembly_gap    527..536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            657..>1702
                     /locus_tag="PLH31_15265"
     CDS             657..>1702
                     /locus_tag="PLH31_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR90586.1"
                     /translation="MRIAQVPPLYEAVPPRLYGGTERVVAHLTDALVELGHDVTLFAS
                     AEARTTAKLVVVRDQAIRLDPAPLKSDLAAHLAMLLDVRDRAEEFDVIHFHTDMLQFG
                     LFASMAGKTLTTLHGRLDLKDLANLYRHFPEFGLVSISDSQRVPLPDANWVGTVLHGL
                     HSDIDTYRAKTDGYLAFLGRISPEKGPERAIAIAKRVGRKLKIAAKIDQADIGYFHQV
                     VEPLLADPLVEFVGEIGDQEKGRFLGGADALLFPIDWPEPFGLVMIGAMAAGTPVIAY
                     DRGSVREVIEEGLTGFIVNNETDAVAAVHRLHQLSRRAIRDRFDLRFSATAMARRYET
                     LYEQMIEAGDVDLN"
BASE COUNT          310 a          583 c          515 g          284 t
ORIGIN      
        1 gaaatcacgc ctgtcagcgc gccggcgcca taggggccag cgcctgcgcc gcgtacgata
       61 tcggcgccgg ccaccccctc cggcgccagc tgcgaccaga tcacccagcc gccgaacgga
      121 tcgttcagcg gggcgccatc cagcagcacc agcgtccgcc cggcccccga aggcgcgatg
      181 gcccgcaggc ttatcccctg cgtggtggga ttggccgaaa ggctggaggt ccgtcggaag
      241 agcgacacgc cgggcgtccg gctcagcgcc tcatcgatac gcacggcccc ggccagttca
      301 gcctcgtcga gccgcaccac ggagaacgcc gcctcgcccc gcaagggcgg caggcgcgcg
      361 gcggagacca caacgggcgc gacagcagca ggcggcagat caaccggcac ggaaatcgac
      421 tttcatcaaa agggtctctg acgtcctgag gcgcaagtca cggccgaatc cgctgccccg
      481 ccaggctctc aacctgcatg gcccgtcgtc ttcccgaaag gcctatnnnn nnnnnnaagc
      541 tcgcacaagg ccgggtagca aagtcagcaa acccccaggc tcaacggaac ctcggagaag
      601 cttcaacggt ttcagaatgc ggccaaattt ctggccgcac aggggaaaca ttctccatgc
      661 gcattgctca ggtccccccg ctgtatgagg ctgttccgcc tcgcctctat ggcggaaccg
      721 aacgcgtggt cgcgcatctg acagacgctc ttgtggagct gggtcacgat gtgaccctgt
      781 ttgccagcgc cgaggcccga accacagcga agctggtcgt ggtccgcgat caggccatcc
      841 gcctcgaccc tgctccgctc aagtcggacc tcgcagccca cctggccatg ctgctggacg
      901 tccgcgatcg cgccgaagaa ttcgacgtca tccacttcca caccgacatg ctgcagtttg
      961 gtcttttcgc ctctatggcg ggcaagaccc tgaccaccct gcatggccgg ctggatctga
     1021 aggacctggc caacctttat cgacactttc cggaatttgg cctggtttcg atttccgact
     1081 cccagcgcgt gccgctgccg gacgccaact gggtgggcac cgtgctgcac gggctgcatt
     1141 cggatatcga cacctatcgc gcgaaaaccg atggctatct ggccttcctc ggccggattt
     1201 cgcctgagaa aggtcccgag cgcgccatcg caatcgccaa gcgcgtcggg cgaaagctga
     1261 agatcgcggc caagatcgac caagccgaca tcggctattt tcatcaggtg gttgagcccc
     1321 tgctcgccga cccgctggtc gagttcgtcg gcgagatcgg ggaccaggaa aaaggcaggt
     1381 tcctgggcgg ggcggacgcc ctgcttttcc ccatcgactg gcccgagcct ttcggcctgg
     1441 tgatgattgg ggccatggcg gctggcaccc cggtgatcgc ctatgaccgc ggctcggtcc
     1501 gcgaggtgat cgaggagggc ctcaccggct ttatcgtcaa caacgagacc gatgccgtgg
     1561 cggccgtgca ccgcctgcac caactgagcc ggcgcgccat tcgcgaccgc tttgacctgc
     1621 gcttctcggc cacggccatg gcccgtcggt atgagacgct ctacgaacag atgatcgagg
     1681 caggcgatgt cgatctaaac tt
//
LOCUS       DAOMEO010000779         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7245_length_1701_cov_2.242406, whole genome shotgun sequence.
ACCESSION   DAOMEO010000779 DAOMEO010000000
VERSION     DAOMEO010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7245_length_1701_cov_2.242406"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLH31_15270"
     CDS             <1..364
                     /locus_tag="PLH31_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90587.1"
                     /translation="FVIEPQSDGFGFVNLAKFKAGFAADINDADAAFMRDSQVPIAMS
                     AFGDKVTKAAWRAKPSWFVVATEDGAIDPKLLRKTATRIGAEAVEVKGSHVVFLSRPQ
                     AVADMIDKAAKSAVAKAK"
     gene            484..1662
                     /locus_tag="PLH31_15275"
     CDS             484..1662
                     /locus_tag="PLH31_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HQR90588.1"
                     /translation="MTFRTPPVTAWSAALIAALIGFGGTVALVVQAMRVLGGSPEQTG
                     SAVTALCLAIGLAGGALSLGLRMPVVLAWSTPGAALLAGTTAQVSWPVAIGVFLVAAL
                     LAIVLGLVPALGRLAEKIPSAVASAMLAGVLLPFCLTLFRTAAVDPVLVGVILAVFLA
                     ARQRAPLYALLLALAAGVALTLFRGGLSLPESGQILGMLRPQGPVFEPAAIVSLALPL
                     FLVTLVSQNLPGLVVLRTAGYRPPPRPLLAGVGLASLLAAPFGAHGVNLAAITAAICT
                     NPDAHPDAAQRWGVGVIYAGFYLLLALFSPFLVGVFLALPHAVIAALTGLALIPALLG
                     ALEGMLAPAADRDPAIVTFLATASGLSLLGIGSAFWGLMAGFFALGARALLRRQSRRT
                     "
BASE COUNT          200 a          623 c          565 g          313 t
ORIGIN      
        1 cttcgtgatc gagccgcaat cggacggctt cggcttcgtc aacctggcca agttcaaggc
       61 tggcttcgcc gccgatatca acgacgccga cgccgccttc atgcgcgact ctcaggtgcc
      121 gatcgccatg tccgccttcg gcgacaaggt caccaaggcc gcctggcgcg caaagcccag
      181 ctggttcgtc gtggcgaccg aagacggcgc catcgatccg aagctgctgc gcaagaccgc
      241 cacccgcatc ggcgctgaag ccgtcgaggt gaagggcagc cacgtcgtct tcctcagccg
      301 tccgcaagcg gtggctgaca tgatcgacaa ggccgccaag agcgccgtag ccaaggcgaa
      361 gtaagaccag gaccgaccac ggacgtccga gggtccggcc gccggcgcgc caagcgcgtc
      421 ggcggctgcg gccgtgaacg cggctggaaa cggcggcggc tatgctgtta tgcgataggg
      481 cccatgacct tcagaacgcc tcccgtcacc gcctggtccg ctgccctgat cgccgccctg
      541 atcgggttcg gcggcacggt ggcgctggtc gtccaagcca tgcgcgtgct gggcggcagc
      601 cccgagcaga ccggatcggc cgtcaccgcc ctttgcctgg ccatcggcct cgccggcggg
      661 gccctgtcgc tgggcctgcg gatgccggtc gtcctggcct ggtcgacgcc cggcgcggcc
      721 ctgctggcgg ggaccaccgc acaggtttcc tggcccgtcg ccatcggcgt cttcctggtc
      781 gcggccctgc tggccatcgt gctcggcctg gttccggctt tgggccggtt ggccgagaag
      841 atccccagcg ccgtcgcctc ggccatgctg gctggcgtcc tgctgccatt ctgcctgacg
      901 ctgtttcgaa ccgccgcggt ggatcccgtc ctggtcggcg tgattctggc ggttttcctg
      961 gcggcccgcc agcgtgcgcc gctctatgcg ctgctgctgg ccctggcggc gggcgtggcg
     1021 ctgaccctgt ttcgcggcgg cctttccctg ccggagtcag gccagatcct cggaatgctg
     1081 cggccccagg gaccggtctt cgaacccgcc gcgatcgtca gcctggccct gcccctcttc
     1141 ctggtcacct tggtctcgca gaacctgccc ggcctggtgg tgctacgcac ggccggctat
     1201 cggccgcccc cgcgcccgct cctggccgga gtgggcctgg cctccttgct ggccgcgccg
     1261 ttcggggcgc atggggtgaa cctggcagcc attaccgcgg ccatctgcac caacccggac
     1321 gctcaccccg acgcggcgca gcgatggggg gtcggcgtaa tctatgcggg cttttacctg
     1381 ctgctggcgc tgttctcgcc gtttctggtc ggcgtctttc tggcgctgcc tcacgcggtc
     1441 atcgccgcct tgaccggcct tgccctgatc cccgctctgc tgggcgcgct ggaaggcatg
     1501 ctggcgccgg ccgccgaccg cgatccggcc atcgtcacct tcctggccac ggcatctgga
     1561 ctgtcattgc tgggcatcgg ttcggcattc tggggcctga tggcgggctt cttcgccctc
     1621 ggcgcccgag cgctgctacg tcgccaaagc cgcaggacct agaggcgtac gtctttcacg
     1681 tcttgtttgg cgccgccgca c
//
LOCUS       DAOMEO010000780         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7249_length_1700_cov_2.356231, whole genome shotgun sequence.
ACCESSION   DAOMEO010000780 DAOMEO010000000
VERSION     DAOMEO010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7249_length_1700_cov_2.356231"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLH31_15280"
     CDS             complement(<1..737)
                     /locus_tag="PLH31_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HQR90589.1"
                     /translation="MRLRAKLYIPRQIKRWLPTSLFGRSLLIIVLPVAVMQIAVTWAF
                     FDAHWQTVNSRLSEGLAGDVAWAVASYQDDPSPAAVDKLADRAEESLALSIAFQKGKT
                     LPTGRRTSLLPSLDRSLQDALADRLNAPFWFDTTRYTAYIDIRVQVDGGVLQIYALRD
                     RAFATQGHIFILWMVVATMLLTAVAILFIRNQVRAIERLADAADAFGRGGDIDFKPHG
                     AREVRQAAQAFLAMKARIQRHIEQRTAL"
     gene            complement(737..1438)
                     /locus_tag="PLH31_15285"
     CDS             complement(737..1438)
                     /locus_tag="PLH31_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90590.1"
                     /translation="MSPEERRDRHLLVVDDDDRIRKLLKEFLVRAGFRVSTAADAAGA
                     DKLCAALDFDLMVLDVMMPGEDGFAFTKRLRAMGGEVAKTPILMLTARDQAADRIEGL
                     THGVDDYLGKPFEPQELLLRIEAILRRTAAKPSGPRELSLGRCSFEPERGELTCDGQP
                     VRLTEAEVALMRRLARSAHEPVDRLELARDTADTTGRAIDVQVTRLRRKIEPDPKNPR
                     YLQTVRGIGYRLAPD"
     gene            complement(1435..>1700)
                     /locus_tag="PLH31_15290"
     CDS             complement(1435..>1700)
                     /locus_tag="PLH31_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR90591.1"
                     /translation="AASRTLSDLTKAGLVERASGDLDGRRKPAVLTAQGVAFEARTAE
                     RLRTLLAKAYRTGGLDGVAGTRRILAALAGPRQGVGPTRRVVA"
BASE COUNT          258 a          572 c          630 g          240 t
ORIGIN      
        1 agcgcggtgc gctgctcgat atggcgctgg atgcgggcct tcatcgccag gaaggcctgg
       61 gccgcttggc gcacctcgcg cgcgccgtgc ggcttgaagt cgatatcgcc gccccgccca
      121 aaagcgtcgg ccgcgtcggc cagccgctcg atggcccgca cctggttgcg gatgaacagg
      181 atggcgacgg ctgtcagcag catggtcgcc accaccatcc acaggatgaa gatgtggccc
      241 tgggtggcga aggcccggtc gcgcagggcg tagatctgca agaccccgcc gtccacctgc
      301 acgcggatgt cgatataggc ggtatagcgg gtggtatcga accagaacgg cgcgttcagc
      361 cggtcagcca gggcgtcctg cagcgagcgg tctagcgacg gcagaagcga ggtccgccgc
      421 ccggtcggca gcgtcttgcc cttctggaag gcgatggaga gggccaggga ttcctcggcc
      481 cggtcggcca gcttgtcgac ggcggcgggg ctgggatcgt cctgatagct ggccacggcc
      541 caggcgacgt caccggccag gccctcggag aggcggctgt tcaccgtctg ccagtgggcg
      601 tcgaaaaagg cccaggtgac ggcgatctgc atcacggcca cgggcaggac gatgatcagc
      661 agcgagcggc caaacagcga ggtcggcagc cagcgcttga tctggcgcgg gatgtagagc
      721 ttggcgcgca ggcgcatcaa tccggcgcca gccgataccc aatgccccgc accgtctgca
      781 gatagcgcgg gttcttcgga tccggctcga tcttgcggcg caggcgggtg acctggacgt
      841 cgatggcgcg tccagtggtg tcggcggtgt cgcgggccag ttccaggcgg tcgacaggct
      901 cgtgggccga gcgggccagg cggcgcatca gggcgacctc ggcctcggtc aggcgcacgg
      961 gctggccgtc gcaggtcagc tcgccacgct cgggctcgaa ggagcagcgg cccagcgaca
     1021 gctcgcgcgg accgctgggc ttggcggcgg tgcggcgcag gatggcctcg atacgcagca
     1081 gcagctcctg cggctcgaag ggcttgccca gatagtcgtc gacgccgtgg gtcaggccct
     1141 cgatccggtc agcggcctgg tcgcgggcgg tcagcatcag gatcggagtc ttggcgacct
     1201 cgccgcccat ggcccgcagg cgcttggtga aggcaaagcc gtcctcgccg ggcatcatca
     1261 cgtccagcac catcaggtca aagtccagcg ccgcgcacag cttgtcggcc ccggccgcgt
     1321 cggcggcggt gctgacccgg aaaccggccc ggaccaggaa ctccttcagg agcttgcgga
     1381 tgcggtcgtc gtcatcgacc accagcaggt gacgatcgcg gcgttcctcg gggctcatgc
     1441 aaccacccgg cgggtggggc ccacgccctg acgcggaccg gccagggccg ccaggatgcg
     1501 ccgcgtgccg gccacgccgt ccaggccccc ggtgcgatag gccttggcca gcagggtgcg
     1561 cagacgctcg gcggtgcgag cctcgaaagc cacgccctgc gcggtcagca cggcgggctt
     1621 gcggcggccg tccagatcgc ccgaggcgcg ctcaaccagc ccggccttgg tcaggtccga
     1681 gagggtgcgg ctggcggcct
//
LOCUS       DAOMEO010000781         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7257_length_1699_cov_1.650243, whole genome shotgun sequence.
ACCESSION   DAOMEO010000781 DAOMEO010000000
VERSION     DAOMEO010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7257_length_1699_cov_1.650243"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLH31_15295"
     CDS             complement(<1..302)
                     /locus_tag="PLH31_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQR90592.1"
                     /translation="MVKLMALVKKPVRKRTAKRISPAERERVAVLFDRFESLELRPKT
                     ELNYNNPYELVTAVALSAQATDVQVNKSTGPLFQVAGSPEAMLALGEEGLIRYIAS"
     gene            355..861
                     /locus_tag="PLH31_15300"
     CDS             355..861
                     /locus_tag="PLH31_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HQR90593.1"
                     /translation="MAGPLYMDAVIKPNRSLSRRGLHVVLGVVLAFNLIIALFVLGVG
                     APLVLPFLGLDVAAIWLAMNASFRAAERRERVRVTAEAITVSREDEKGARLVWTSPTA
                     FTGVDVDQPGEHEARVRLRIYRRRLTVARALSPPERVEFADALRRAIREARAERYAAP
                     GAGAGAPA"
     gene            909..>1699
                     /locus_tag="PLH31_15305"
     CDS             909..>1699
                     /locus_tag="PLH31_15305"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQR90594.1"
                     /translation="MALDMHPSETFARLSERSADYHRMAVALDWLAERWQERPSLDEA
                     AGAVGLSPFHFQRIFTRWAGVSPKTFVAAIAHAEARKSLEAGASVLDAAYDAGLSGPS
                     RLHDLFIAQEAATPGEVRRRGAGMALRYGWAPTPFGKGLFVMAERGLAGLAFSDGDDE
                     ATFADMHRRFPAADWVCDDEAASATAIHAFAGGQGPLPVVLIGSPFHIQVWKALLRIP
                     VGATGTYGQVAEWAGKPRAYQATGGAIGANPISLLIPCHRAIAKDG"
BASE COUNT          237 a          576 c          596 g          290 t
ORIGIN      
        1 atcgaggcga tgtagcggat caggccttcc tcgccgagcg ccagcatggc ctcggggctg
       61 cctgcgacct ggaacagcgg cccggtggac ttgttgacct gaacatcggt ggcctgggcc
      121 gagagcgcga ccgccgtcac cagctcatag gggttgttat agttcagctc ggtcttgggc
      181 cgcagttcca ggctctcgaa acgatcgaac agcaccgcca cccgttcacg ctcggctggc
      241 gagatccgct tggcggtgcg tttgcggaca ggttttttca caagagccat taacttgacc
      301 atgccgtgat gcgaccccct cgccaaggcg gcgatgcatg cctaaatcga tgacatggcc
      361 gggccgcttt acatggacgc cgtgatcaag cccaaccggt cgctcagccg gcggggcctt
      421 cacgtggtgc tgggcgttgt tctggccttc aacctgatca ttgcgctgtt tgtcctgggg
      481 gtgggcgcgc cgctggtgct gccgtttctg ggtctggacg tggcggcgat ctggctggcc
      541 atgaacgcca gtttccgggc cgccgagcgt cgcgaacggg tgcgggtcac cgccgaggcg
      601 atcacggtca gccgcgagga cgaaaaaggc gctcgtctgg tctggacttc gcccaccgcc
      661 tttaccggcg tggatgtcga tcagccgggc gagcatgagg cgcgggttcg gctgcgcatt
      721 tatcgccgcc gcctgaccgt ggcccgggcg ctcagtccgc ccgaacgggt ggagttcgcc
      781 gacgccctgc ggcgcgccat ccgcgaggcg cgcgccgagc gctatgccgc gccgggggct
      841 ggagccggcg cgccagcgtg agcaagaacc gggcggacac gcctgcggag ggcgcatacg
      901 gtcccgccat ggctctcgac atgcacccct cagaaacctt cgcccgtctg tccgagcgct
      961 cggcggacta tcaccgcatg gcggtggccc tcgactggct ggccgagcgc tggcaggagc
     1021 gcccgtcgct ggatgaggcc gccggcgcgg tcggcctgtc gccgttccac ttccagcgga
     1081 tcttcacccg ctgggccggg gtcagtccga agacctttgt tgcggccatc gcccatgccg
     1141 aggctcgcaa gtcgctggaa gccggcgcca gcgtgctgga tgcggcctat gacgccgggc
     1201 tttccggtcc ctcgcgcctg cacgacctgt tcatcgccca ggaagccgcc acaccgggcg
     1261 aggtgcggcg tcgcggagcc ggtatggcct tgcggtatgg ctgggcgccc acgccctttg
     1321 gcaagggcct gttcgtgatg gccgagcgcg gcctggcggg cctggccttt tcggacggcg
     1381 acgacgaagc gaccttcgcc gacatgcacc gccgcttccc ggccgccgac tgggtctgcg
     1441 acgatgaggc ggctagcgcg acggcgatcc acgcctttgc gggcgggcag gggcccctgc
     1501 cggtggtgct gatcggctcg ccgttccaca tccaggtctg gaaggccctg ctccgcatcc
     1561 cggtcggcgc gaccggcacc tatggccagg tggcggaatg ggccggtaag cccagggcct
     1621 accaggcgac gggcggggca atcggggcca atccgatctc gctattgatc ccctgccacc
     1681 gcgccatcgc caaggacgg
//
LOCUS       DAOMEO010000782         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7270_length_1696_cov_2.576478, whole genome shotgun sequence.
ACCESSION   DAOMEO010000782 DAOMEO010000000
VERSION     DAOMEO010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_1696_cov_2.576478"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..512
                     /locus_tag="PLH31_15310"
     CDS             264..512
                     /locus_tag="PLH31_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HQR90595.1"
                     /translation="MGSLKKRSVNLAGHATSLALEPEFWSVLERAAIDDALSLAALIR
                     LIDTSRGERPLASACRVFALQRALADQPGRGAVGSGGG"
     gene            complement(694..888)
                     /locus_tag="PLH31_15315"
     CDS             complement(694..888)
                     /locus_tag="PLH31_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90596.1"
                     /translation="MTEGTGNSGGGNSALAFIVGGLVVIVAVIAFFVYSGGVSQKKSV
                     DVNISASAPKLPDAPKLPGN"
     gene            998..>1696
                     /locus_tag="PLH31_15320"
     CDS             998..>1696
                     /locus_tag="PLH31_15320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQR90597.1"
                     /translation="MSSGFDSPPPHGDLPSPRISDLARPRGPAGGDPGYLDGLNPEQR
                     AAVETTEGPVLVLAGAGTGKTRVLTTRLAHILSTGRARPWELLAVTFTNKAAREMRER
                     ITHIIGSEAEGLRWLGTFHSVAAQILRRHAELVGLKSSYTIIDTDDQERLLKQLITAE
                     NIDAKRWTPKILAGMIDNWKNKGWTPDRVPPAEGGEFANGKGVTLYRQYQERLRILNA
                     CDFGDLLLHNITIFT"
BASE COUNT          297 a          588 c          531 g          280 t
ORIGIN      
        1 ttttggtggg cggactctaa ttattcctgg aggagtttca gggggtcacg tcaccgctcg
       61 gctcgctttg cagttccaga accgtgccat ccacggcgtg ctctacggcc atgcgctcta
      121 ctctgaagga aactctacgc gtccgaagct tccgcgccac ggtctgatta tagggtatgg
      181 cgcgcagtgc aaggcgcgac ggggaacacc ccacaacgca agccggattg cttcaccgcc
      241 cgtcagggcg catttctcct accatgggca gtctcaagaa gcggtcggtg aatctggccg
      301 gccacgccac gtcgctggcc ctggagccgg aattctggag cgtcctggag cgggccgcga
      361 ttgatgatgc gctgagcctc gcggccctga tccggctcat cgatacaagc cgaggcgagc
      421 ggccgttggc ctcggcttgc agggtcttcg cccttcagcg cgcgctggcc gatcaacccg
      481 gtcgcggggc ggtcggcagc ggcggcggct gagggtttgg catcgaccgc gacgggacgg
      541 acggcgcccg aagccgcgga ccggacggaa cggtgaccgt tccgcacaga cgccaggcca
      601 tccacagcgt caacatcacc acgaccacga ccagccccgc cagccagccg ccatatcggt
      661 catggcgcgg aggggcgtgt ttcacggccg acttcagttt ccaggtagct ttggcgcgtc
      721 cggaagcttg ggcgccgacg ctgaaatatt gacgtcgacc gacttcttct gactgacacc
      781 ccccgaatag acgaagaagg cgatgaccgc gacgatcacc accagacccc caacgatgaa
      841 cgccagcgcg ctgtttccgc cgccgctgtt gcctgtacct tcggtcatag tggcctcccg
      901 tgattgatgt gccggatcaa acgacgccca gcggccaagg ttccgtattc cgcgccgagg
      961 ggcttaagct ggcggccgcg tgtgctacat atgttccatg tcctcaggct tcgactcgcc
     1021 gcctccgcat ggcgacctcc cctctccgcg catctccgat ctggccaggc ctcgcggtcc
     1081 ggctggcggc gaccccggct atctggacgg gctgaacccc gagcaacggg cggcggtcga
     1141 gaccaccgag ggccccgtcc tggtcctggc cggagccggc accggcaaga cccgggtgct
     1201 gaccacccgc ctggcccaca tcctgtcgac cggccgcgcc cgcccgtggg agctgctggc
     1261 cgtcaccttc accaacaagg ccgcccgcga gatgcgtgag cggatcaccc acatcatcgg
     1321 gtccgaggcc gagggcctgc gctggctggg caccttccac tcggtggccg cccagatcct
     1381 gcgccgtcac gccgagctgg tgggtctgaa atccagctac accatcatcg acaccgacga
     1441 tcaggagcgg ctgctcaagc aactgatcac ggccgagaat atcgacgcca agcgctggac
     1501 gcccaagatc ctggcgggga tgatcgacaa ctggaagaac aagggctgga cgcccgaccg
     1561 cgtgcccccg gccgagggcg gcgagttcgc caacggcaag ggcgtcaccc tgtatcgtca
     1621 gtatcaggag cggctgcgca tcctgaacgc ctgcgatttc ggcgacctgc tgctgcacaa
     1681 catcacgatc ttcacc
//
LOCUS       DAOMEO010000783         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7294_length_1692_cov_2.245571, whole genome shotgun sequence.
ACCESSION   DAOMEO010000783 DAOMEO010000000
VERSION     DAOMEO010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7294_length_1692_cov_2.245571"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLH31_15325"
     CDS             complement(<1..1382)
                     /locus_tag="PLH31_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQR90598.1"
                     /translation="MNAPFKTDALPEWNLTDLYADRDDPRIEVDLAAAKAANDELASL
                     EGLFLQARGEPERLGLLIDRGIRLYEKATNHLWSVGAYASLAGSVAHDDPACAKFEAD
                     LRARSAGIAAESLFFTLELNKLEDSEIQAALAARPAAQRWTPWLRRVRLSRPHELSPD
                     LERFIVDKSPAAANWVRLYDETLAKLQAKVGDESLTLPETLNRLSDSDVARRKAAADG
                     LAAALEARVSTQALVLNTLAFEKQVEDRWRRYAEPAQARHLSNEVDADAVNALEAAVV
                     EAYPRLSHRYYALKAKIMGVDALDYWDRNAPLDAAAPRTYDWAEAKDVVLDSFQTLAP
                     KFADSARTFFNQPWIDARPRPGKQSGAYAHPVTAERHPYVFMNYMGERRDVLTLAHEL
                     GHAVHQTLCAPLGTLLADTPLTLAETASIFGEGLVFDRLLAEATPDDRKKLLAGKIED
                     GLNTVVRQIA"
BASE COUNT          247 a          544 c          621 g          280 t
ORIGIN      
        1 aaggcgatct ggcggaccac ggtgttgagg ccgtcctcga tcttgccggc cagcagcttc
       61 ttgcgatcgt cgggcgtggc ctccgccagc aggcggtcga acaccagccc ctcgccgaag
      121 atcgaggccg tctcggccag ggtcaggggc gtatcggcca gcagggtgcc cagcggcgcg
      181 cacagggtct ggtgcacggc gtggcccagc tcgtgggcca gggtcaggac gtcccgtcgc
      241 tcgcccatat agttcatgaa cacatagggg tggcgctcgg ccgtcaccgg atgggcatag
      301 gcccccgact gcttgccggg gcggggacgg gcgtcgatcc agggctggtt gaagaaggtc
      361 cgggcggagt cggcgaactt cggagccagg gtctggaaac tgtccagcac cacatccttg
      421 gcttcggccc agtcataggt gcgcggcgcg gcggcatcca gcggcgcgtt gcggtcccag
      481 tagtccaggg cgtccacgcc catgatcttc gccttcagcg catagtagcg gtgcgagagg
      541 cgcggatagg cctcgaccac cgccgcctcc agggcgttga cggcgtcggc gtcgacctcg
      601 ttggacaggt ggcgggcctg ggccggctcg gcatagcggc gccagcggtc ctcgacctgc
      661 ttttcgaagg ccagcgtgtt gagcaccagc gcctgggtcg agacgcgcgc ttccagagcc
      721 gcggccaggc catcggcggc cgccttgcgg cgggcgacgt cggagtcgga caggcgattg
      781 agggtttcgg gcagggtcag gctctcgtcg ccgaccttgg cctgcagctt ggccagggtc
      841 tcgtcataga gacgcaccca gttggcggcg gccgggctct tgtcgacaat gaaccgctcc
      901 agatcgggcg acagctcgtg cggccgcgac aggcgcacgc ggcgcagcca cggcgtccag
      961 cgctgggcag cggggcgcgc ggccagggcg gcctggatct cgctgtcttc cagcttgttg
     1021 agctccaggg tgaagaacag gctttcggcg gcgatcccgg ccgagcgggc ccgcaggtcg
     1081 gcctcgaact tggcgcaggc cggatcatca tgcgccaccg agccggccag gctggcatag
     1141 gcgccgacac tccacaggtg gttggtggct ttctcgtaca gccggatgcc gcgatcgatc
     1201 agcaggccca gccgctcggg ctcgccgcgc gcctgcagaa acaggccctc cagcgaggcc
     1261 agctcgtcat tggcggcctt ggcggcggcg aggtccacct cgatgcgggg gtcgtcgcga
     1321 tccgcataga ggtcggtcag gttccactcg ggaagggcgt cggtcttgaa gggggcgttc
     1381 atgatcacag ggatatggcc cctgcggggt ggggcgcaac ggcgcatggc cgttgggggc
     1441 gacattccag ggttcatggc ctcgcgcttt tgctgcgatg gggatgggaa ggggcgcgga
     1501 gcgtccgggc ctcgcccgga taggagtgtt tttaagtacc gtttcgacga agacagacgc
     1561 ctcgcgtgat cgttatccct ctggccgggg cgcgcaggtc tggttcaacc ttttgcccca
     1621 cccgggcttg ttcctcctcg cgacggaccc agcccctaaa gctgcaagcc acgccgattc
     1681 cggaggaccc gg
//
LOCUS       DAOMEO010000784         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7303_length_1691_cov_2.066015, whole genome shotgun sequence.
ACCESSION   DAOMEO010000784 DAOMEO010000000
VERSION     DAOMEO010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7303_length_1691_cov_2.066015"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLH31_15330"
     CDS             complement(<1..756)
                     /locus_tag="PLH31_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HQR90599.1"
                     /translation="MTAVAPPAATPWRLVLLLGALTAFAPMSIDMYLSSLPSIGRSLN
                     AGPDQTQATLAAFFAGMAIGQFIYGPASDRFGRRGPILFGVGVYFIASVACALAPSIE
                     ALITSRFIQALGGCAGAVVARAVVRDRFSHADTARVLSLMTLIMGLAPVLAPQLGGII
                     QLIAGWRAVFWTLAVFGLAIGVWVALGLSESRSAETEAQARSENPLKAFGALLRMRRL
                     VGYGLAGTLNGAVLFSYISGAPEVIMGTYGHSPL"
     gene            912..>1025
                     /locus_tag="PLH31_15335"
     CDS             912..>1025
                     /locus_tag="PLH31_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90600.1"
                     /translation="MPPPSFATVNGIRMAYYEAGPRQGVPIVLCHGFPEFSY"
     assembly_gap    1026..1035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1036..>1691
                     /locus_tag="PLH31_15340"
     CDS             <1036..>1691
                     /locus_tag="PLH31_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR90601.1"
                     /translation="AAAGRWVIVPDQRGYGLTDRPEAVEAYDMEHLTGDLVGLLDHLG
                     VEKGVFCGHDWGGIIVWAMPLLHPGRCAGIIGLNTPFTPRLPMDPIEMYRRAYGDEMY
                     IVHFQKPGIADAQLGADPEKTIRYFMRLPGDSVEAFEARPAEKRSLALQEGLARYDPA
                     TDPNQVLRPEEAAVFAETFTRTGFTGGINWYRNFTRNWERSEHLPRRIDGVPCLMIMA
                     "
BASE COUNT          302 a          592 c          551 g          236 t
ORIGIN      
        1 cagcggcgag tggccgtagg tccccatgat cacctccggc gcgccggaga tgtaggagaa
       61 cagcaccgcg ccgttcaggg tcccggccag gccatagccg accagacgcc gcatccgcag
      121 cagcgcgcca aaggccttga gcgggttctc ggaccgggcc tgggcctcgg tctcggccga
      181 tcgcgactcg ctcagcccca gcgccaccca gacgccgatc gccaggccaa acaccgccag
      241 cgtccagaac accgcgcgcc atccggcgat cagctggatg atcccgccca gctgcggcgc
      301 cagcacgggc gcgagaccca tgatcagggt catcagcgac agcacccggg cggtgtcggc
      361 atggctgaac cggtcgcgca ccacggcccg cgccaccacg gcgcccgcgc aaccgcccag
      421 ggcctggatg aagcgggagg taatcagggc ttcgatgctc ggcgccaggg cgcaggccac
      481 cgaggcgatg aaatagacgc ccacgccaaa caggatcggt ccgcgacggc caaagcggtc
      541 agacgcgggg ccatagatga actggccgat ggccatgccg gcgaagaagg ccgccagcgt
      601 cgcctgggtc tggtcgggtc cggcgttcag cgatcggccg atggacggca ggctcgacaa
      661 gtacatgtcg atcgacatcg gggcgaaggc ggtcagggcc cccagcagca gaaccagtct
      721 ccagggtgtg gcggcgggag gagcgacagc ggtcatggcg cgcttctact cccgtttgac
      781 gctaggtgaa cccctaaacc gggcttgccc cgcgcccagc ggaccggcac ggtaatcaac
      841 aatcagatag ggccggaaac tcgcaggaga cgccaatgac cgttcaggaa gagcgcccgt
      901 tcgccccgcc catgccgccg ccgtcgttcg cgacggtcaa cggcattcgc atggcctact
      961 acgaggccgg cccgcgtcag ggcgttccca tcgtgctgtg tcacggcttt cccgagtttt
     1021 cctatnnnnn nnnnngccgc cgcaggtcga tgggtgatcg tccctgacca gcgcggctat
     1081 ggcctgaccg atcggcccga ggccgtcgag gcctatgaca tggagcacct gaccggcgat
     1141 ctggttgggc tgctcgacca tctgggcgtc gaaaaggggg tgttctgcgg ccacgactgg
     1201 ggcggcatca tcgtctgggc catgccgctg ctacatcccg gtcgctgcgc cgggatcatc
     1261 ggcctgaaca cgcccttcac cccccgcctg ccgatggatc cgatcgagat gtaccgccgc
     1321 gcctatggcg acgagatgta catcgtccac ttccagaagc ccgggatcgc cgacgcccag
     1381 ctgggcgccg atccggagaa gaccatccgc tacttcatgc gcctgcccgg cgacagcgtg
     1441 gaagccttcg aggcccggcc tgccgaaaag cgcagcctgg ccctgcagga ggggctggcg
     1501 cgctacgacc ccgccaccga tcccaaccag gttctgcgcc ccgaagaggc ggcggtgttc
     1561 gcagagacct tcacccgcac cggtttcacc gggggcatca actggtatcg caacttcacc
     1621 cgcaactggg agcggtcgga acaccttccg cgccggatcg acggcgtgcc ctgcctgatg
     1681 atcatggccg a
//
LOCUS       DAOMEO010000785         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7324_length_1687_cov_2.526348, whole genome shotgun sequence.
ACCESSION   DAOMEO010000785 DAOMEO010000000
VERSION     DAOMEO010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_1687_cov_2.526348"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1385)
                     /gene="chrA"
                     /locus_tag="PLH31_15345"
     CDS             complement(102..1385)
                     /gene="chrA"
                     /locus_tag="PLH31_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQR90602.1"
                     /translation="MTKAPPSFAEALLVWLKIGCLGFGGPAGQIALLHKTIVEENGWV
                     DEARFTHALSFCMLLPGPEAQQLAVWLGWRLHGVRGGLAAGLLFVLPGLAIMLALSAL
                     YVAYGRSEWAGPVLLGLKAAVVALVLQALLRIGRRTIRDRAGMTVAVAAFALMSFTLA
                     PFPLLILAAGALGWALGGGAVVEGAPEAAGGRRATLPTVMLWLAIWLAPVTAALAIAP
                     DSVVARMGAIFSGLAVVSFGGAYAALAYLGQAASALGWLTPGQMLDGLGLAETTPGPL
                     VLVFVFVGFVGAYQAASPEWAWVAGLAGGLMAAWATFAPSFLWIFAGGPFVERLRARP
                     RPARALSMVSAAAVGVIAQLAVWFATHLLFRTGATQVWGAMRFTLPDPASLDATALGL
                     TTLALGLTFALRLPVLALVTVLVAAALALRAFGLS"
     assembly_gap    1459..1468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1469..>1687)
                     /gene="rlmJ"
                     /locus_tag="PLH31_15350"
     CDS             complement(<1469..>1687)
                     /gene="rlmJ"
                     /locus_tag="PLH31_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HQR90603.1"
                     /translation="LRREDRYTACELRDDDFDLLRRSLAPYAFAQAAKADGYETAAAR
                     AGRGGRAFILIDPPFERPDDYARIVEVTR"
BASE COUNT          319 a          621 c          556 g          181 t
ORIGIN      
        1 actgggtaga aaccatggcg agcgctccgg caaaccgcga tttgtgcatc gcagcataac
       61 acagattttt cgttcgcaac tgcaaaaatc ttgcccctag gtcaacttag accgaaggcc
      121 ctcaaagcca gtgccgccgc caccagcacc gtcaccaggg ccagcaccgg cagacgcaag
      181 gcgaaggtga ggcccagcgc cagcgtcgtc aggcccaggg ctgtcgcatc aagactggcc
      241 ggatcaggaa gggtgaacct catcgccccc cagacctgcg tcgcgccggt gcgaaacagc
      301 aggtgggtcg caaaccagac cgccagttgc gcgatgacgc cgacggcggc cgccgacacc
      361 atcgacaggg cccgggccgg ccggggacgg gcgcgcaggc gctctacaaa cggcccgccc
      421 gcgaagatcc acaggaacga cggcgcaaag gtcgcccacg ccgccatcag gcctccggcc
      481 aggccggcga cccaggccca ctccggcgag gccgcctgat aggcaccgac gaacccgacg
      541 aagacaaaga ccagcaccag cggtccgggc gttgtctcgg ccaggccaag gccgtccagc
      601 atctgccccg gcgtgagcca gcccagggcc gaggccgcct gtcccagata ggccagggcc
      661 gcataggcgc cgccgaagct gaccaccgcc aggccgctga agatcgcgcc catgcgggcc
      721 acgacggagt cgggggcgat ggccagggcc gccgtaacgg gcgccagcca gatcgccagc
      781 cagagcatga cggtcggcag cgtcgcacga cgtccgcctg ccgcttccgg cgcaccctcg
      841 acgaccgcgc cgccgcccag cgcccaccca agggctcccg ccgccaggat cagcagggga
      901 aacggcgcca gcgtgaagct catcagggcg aaggcggcca cagccaccgt catcccggcc
      961 ctgtcgcgga tcgtacggcg gccgatacgc agcagcgcct ggagcaccag ggccacgacg
     1021 gcggccttca ggcccagcag cacagggcca gcccattcgg atcgtccgta ggcgacatac
     1081 agcgccgaaa gcgccagcat gatcgccagg cccggcaaga caaacagcag gcccgccgcc
     1141 aggccgccgc gcacgccatg cagacgccag cccagccaga cggccagttg ctgcgcctcc
     1201 ggccccggca gcagcatgca gaagctcagg gcatgggtga agcgagcctc gtcgacccag
     1261 ccgttttcct caacgatggt cttgtggagc agggcgatct gcccggccgg cccgccaaag
     1321 cccaggcagc cgatcttcag ccagaccagc agggcctcgg caaagctggg cggggccttg
     1381 gtcaccgggc cgtgcgccag accctggccg cgccgcctgc ctgacccaga tggcgcacga
     1441 tccaatcggt ggccgtccnn nnnnnnnngc gggtgacctc cacaatgcgg gcatagtcgt
     1501 ccgggcgctc gaacggcggg tcgatcagga tgaaggcccg cccgccccgc cccgccctgg
     1561 cggcggctgt ctcatagccg tccgccttgg cggcctgggc gaaggcatag ggcgccagtg
     1621 agcggcgtag caggtcgaag tcgtcgtcgc gcagttcgca ggcggtatag cgatcctcgc
     1681 ggcgcag
//
LOCUS       DAOMEO010000786         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7325_length_1687_cov_1.659314, whole genome shotgun sequence.
ACCESSION   DAOMEO010000786 DAOMEO010000000
VERSION     DAOMEO010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7325_length_1687_cov_1.659314"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLH31_15355"
     CDS             complement(<1..273)
                     /locus_tag="PLH31_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR90604.1"
                     /translation="MLYKLQFAELLPYWDVLLQGLVFTIVLTIVSTAAGIAVGTAGAS
                     ARTFGPAWLSWIVVVYVELIRNTPFIVQLFFIFFGLPSLGISLGEMQ"
     gene            complement(299..>821)
                     /locus_tag="PLH31_15360"
     CDS             complement(299..>821)
                     /locus_tag="PLH31_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQR90605.1"
                     /translation="QTHKADLVISSLGKNAERAKVIDFSNAYAPFFSAVYSVKGKDAG
                     ITKLADLSGKTVGAARGTIEEQALSATAPKDATIKRFEDNSATIAAFVSGQIDLVATG
                     NTVAAVIAKQNPARMPEMKFLIKDSPCYVGMNKAEPALMAKVNSIIADAKKAGEIEKL
                     SEKWLKQPLPPGF"
     assembly_gap    822..831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<832..1058)
                     /locus_tag="PLH31_15365"
     CDS             complement(<832..1058)
                     /locus_tag="PLH31_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90606.1"
                     /translation="MTKTGFAALMLASAISLLGLSAGPAHADALADIMKTKVIKIAVP
                     QDFAPFGSAGLDLKPQGYDIDMANLIGKALGV"
     gene            1200..1298
                     /locus_tag="PLH31_15370"
     CDS             1200..1298
                     /locus_tag="PLH31_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR90607.1"
                     /translation="MDLRQLRYFAMVAEELSFTRAAARLRPHLVAV"
     gene            1364..>1687
                     /locus_tag="PLH31_15375"
     CDS             1364..>1687
                     /locus_tag="PLH31_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HQR90608.1"
                     /translation="MTHVQSAPETKVAAPESQSALDTLMRSFETYKETNEIRLAQIER
                     NGAVDPITAEKLARIDGVLDLHTARLDDMSLKSQRPSLGGGRLRAASEREHGDAFSTY
                     VRHGEA"
BASE COUNT          297 a          530 c          535 g          315 t
ORIGIN      
        1 ttgcatctcg ccgagcgaga tgcccagcga cggcaggccg aagaagatga agaacaactg
       61 gacgatgaac ggcgtgttgc ggatcagctc gacatagacg acgacgatcc agctgagcca
      121 agccggcccg aacgtccgcg cggatgcccc cgccgtgccg accgcgatac cggccgccgt
      181 cgacacgatc gtcagtacga tcgtgaagac gaggccctgc aacagaacgt cccagtaggg
      241 gagcagttcg gcgaattgca gtttgtacag catggcgggg tagccccgcg ccgcgcgatc
      301 agaagcccgg cggcaaaggc tgcttcagcc acttctccga cagcttctcg atctcgccgg
      361 ccttcttggc atcggcgatg atgctgttga ccttggccat cagcgccggc tcggccttgt
      421 tcatgccgac gtagcagggg ctatccttga tcagaaactt catctccggc atgcgcgccg
      481 ggttctgctt ggcgatcacg gcggccaccg tgttgccggt cgcgaccaga tcgatctgac
      541 cggacacgaa ggcggcgatc gtggcgctgt tgtcctcgaa gcgcttgatg gtggcgtctt
      601 tcggcgcggt ggcgctcaaa gcctgctctt caatggtgcc gcgcgcggcg ccgacggttt
      661 tacccgacag gtcggcaagc ttggtgatgc cggcatcctt gcccttcaca ctgtagacgg
      721 ccgagaagaa cggcgcgtag gcattcgaga agtcgatgac cttggcgcgt tcagcattct
      781 tgccgaggct ggagatgacg aggtcggcct tgtgggtttg cnnnnnnnnn nacgccgagc
      841 gccttgccga tcaaattggc catgtcgatg tcgtagccct gcggcttgag atcgaggccg
      901 gcggagccga acggcgcgaa atcctgcggc acggcgatct tgatcacctt ggtcttcatg
      961 atgtcggcaa gcgcgtcggc atgggccggg ccggcggaca gtcccaggag gctgatggcg
     1021 gaagcgagca tcagggcggc gaagccggtt ttggtcacgc gcttaagcat gcgtagattc
     1081 cttgttttgg atcgcaatcg ggccggacga caccccggcc gcgtcatcct cgcatatcta
     1141 ttgcagtgcg gaatacacag gtccaatata ttgttagtgg caatcaatag gtttttcgta
     1201 tggatctgcg ccagctccga tacttcgcca tggtcgccga ggagctgagc ttcacccgcg
     1261 ccgccgcccg gctgcgcccg catctggtgg cggtctgagc cgtcttacca ccccgcgccg
     1321 ggcgcgcggg ccttcacttt cctcccgacc ataggatgtc ttcatgaccc atgttcagtc
     1381 cgccccggaa accaaggtcg ccgcgcccga gagccagtcg gcgctcgata cgctgatgcg
     1441 ctcgttcgag acctataagg agaccaacga gatccgcctg gcgcagatcg agcgcaacgg
     1501 cgcggtcgat ccgatcaccg ccgagaagct ggcgcgcatc gatggcgtgc tggatctgca
     1561 caccgcacgc ctcgacgaca tgtcgctcaa gagccagcgt ccgtccctgg gcggtgggcg
     1621 cctgcgcgcc gcctccgagc gcgagcatgg cgatgccttc tccacttatg tccgccatgg
     1681 cgaggcc
//
LOCUS       DAOMEO010000787         1683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7341_length_1683_cov_2.621622, whole genome shotgun sequence.
ACCESSION   DAOMEO010000787 DAOMEO010000000
VERSION     DAOMEO010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7341_length_1683_cov_2.621622"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PLH31_15380"
     CDS             <1..134
                     /locus_tag="PLH31_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90609.1"
                     /translation="SRESGGSGLGLAVVKAIAEAHGGTVSAANRPDGGAVLELRLPA"
     gene            complement(146..946)
                     /locus_tag="PLH31_15385"
     CDS             complement(146..946)
                     /locus_tag="PLH31_15385"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQR90610.1"
                     /translation="MPDWLIAIVLGLIEGLTEFIPVSSTGHLLLVGHFLGFNSPGNTF
                     QVLIQLGAILAITGVYFGRLWGLLTTAPTDPGARRFILGVFLAFLPAVFVGVLAHDFI
                     KTVLYESPLLVCITLITGGFALLALDRMKIEPEHTDVADYPLKTAFIIGLFQCLALVP
                     GVSRSGATIAGALLLKCDKRSAAEFSFFLAMPTMAGAFAYDLYKNIDKLSTNDLGLIG
                     LGFVAALISGVFVVKTVLDFVSKHGFAPFAWWRIAVGVVGLGLLYIPH"
     gene            1179..>1683
                     /locus_tag="PLH31_15390"
     CDS             1179..>1683
                     /locus_tag="PLH31_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90611.1"
                     /translation="MAERMLKFTTVARKTPEKRAAEARNGDFHEIYADFIDAKATEQA
                     SRCSQCGVPFCQTHCPLHNNIPDWLRMTAEGRIQEAYELSQATSSMPEVCGRICPQDR
                     LCEGNCVIEQSGHGTVTIGSVERYLSDKAWEMGWVKPLVAGPARGQSVGIIGAGPAGL
                     STAEVLRE"
BASE COUNT          346 a          529 c          551 g          257 t
ORIGIN      
        1 ggtcgcggga gtcaggcggc agcggcctgg gtctcgcggt ggtcaaggcc atagcggagg
       61 cccacggcgg gacagtctcg gcagccaacc ggcccgatgg cggcgcggtg cttgagctgc
      121 gcctgccagc ctgagcggcc cttcgtcagt gcggtatgta gagcagaccc agccccacaa
      181 cgcctaccgc gatgcgccac caggcgaacg gcgcaaagcc gtgcttgctg acaaagtcca
      241 gcacggtctt gacgacgaag acgcccgaaa tcagcgccgc tacgaagccc aggccgatca
      301 ggcccaggtc gttggtgctg agcttgtcga tgttcttgta gaggtcatag gcgaaggcgc
      361 cggccatggt tggcatggcc aggaagaagc tgaactcggc cgccgagcgc ttgtcgcact
      421 tcagcagcag ggcgccggcg atcgtggcgc ccgagcgcga gacgcccggc accagggcca
      481 ggcactggaa caggccgatg ataaaggcgg tctttagcgg atagtcggcc acatcggtgt
      541 gctcgggctc tatcttcatc cggtcgagag ccagcagggc gaagccgccg gtgatcaggg
      601 tgatgcagac cagcagcggc gactcgtaga gcaccgtctt gatgaagtcg tgcgccagca
      661 ccccgacaaa gaccgccggc agaaaggcca ggaagacacc caggatgaag cggcgcgcgc
      721 caggatcggt cggcgccgtg gtcagcagtc cccagagacg gccaaaatag accccggtaa
      781 tggccaggat cgcgcccagc tggatcaata cctggaaggt gtttccggga ctgttgaagc
      841 ccaggaaatg cccgaccagc aggaggtgtc cggtcgagga aacgggaatg aactcggtca
      901 gcccctcgat caggcccaac acgatcgcga tcagccaatc tggcatcggc actcccccaa
      961 gacgcaggcc ctcggcgtgc gcctgacacg gtaaacaagg cgttcatcca caaagtggac
     1021 caatatggct atgggctgcg aaatgttcgc aagggccccg cgacgcgcag ggatataatc
     1081 gtcaattatt tttaccaccg cattcttctt gccccaaaga gtccgtatga ggccgccata
     1141 cgcaattttt ctcccagagc tgggacgagg gaagtcgcat ggccgagcgc atgctgaaat
     1201 tcaccacggt ggcccgcaag acgcccgaaa agcgtgcggc cgaagcccgt aatggcgact
     1261 tccacgagat ctacgccgac ttcatcgacg ccaaggcgac cgagcaggcc tcgcgttgct
     1321 cgcaatgcgg cgtgccgttc tgtcaaactc actgcccgct gcacaacaac atccccgact
     1381 ggctgcggat gaccgccgag ggccggatcc aggaagccta tgagctgtcg caggccacca
     1441 gctcgatgcc cgaggtctgc ggtagaatct gcccccagga ccgtctgtgc gaaggcaact
     1501 gcgttataga gcagtcgggt catggcaccg tgacaatcgg ctcggtcgag cgctacctct
     1561 ccgacaaggc ctgggagatg ggctgggtca agccgctggt cgccggtccg gcgcgcggcc
     1621 agtctgtcgg gatcatcggc gcgggccccg cgggcctgtc gacggccgag gtgctgcgcg
     1681 agc
//
LOCUS       DAOMEO010000788         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7367_length_1679_cov_2.380542, whole genome shotgun sequence.
ACCESSION   DAOMEO010000788 DAOMEO010000000
VERSION     DAOMEO010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7367_length_1679_cov_2.380542"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="ilvB"
                     /locus_tag="PLH31_15395"
     CDS             complement(<1..1331)
                     /gene="ilvB"
                     /locus_tag="PLH31_15395"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQR90612.1"
                     /translation="MTAHQTIESPAPSETIDRAMTGAEIVVRGLVDQGVEVLFGYPGG
                     AVLPIYDALFHEPRLQHILVRHEQGATHAAEGYARSSGKPGVVLVTSGPGATNAITGI
                     MDALMDSIPMVVITGQVPTHLIGTDAFQEADTVGMTRSCTKHNYLVKDVRDLPQIIHE
                     AFKIATTGRPGPVLIDIPKDVQFAKGEYYGPTEVASTHAYNPRTKGEPNRIAEAAAMI
                     ARARRPIFYTGGGVINAGPKASAALREFQALTGAPVTSTLMGLGAFPAADPAWLGMLG
                     MHGTFEANNAMHDCDVMICVGARFDDRVTGRLDAFSPGSKKIHIDIDASSINKNVRVD
                     LPIIGDAASVLEDMIAAWKAAGHQPNKAALTDWWAQIDTWRARQCLKYRTSETTIKPQ
                     YAIERLYELTKGRDTYITTEVGQHQMWAAQFYGFQEPNRWMTSGGLGTMGYG"
BASE COUNT          263 a          492 c          612 g          312 t
ORIGIN      
        1 ccataaccca tggtgccgag gccgccggaa gtcatccagc ggttcggctc ctggaagccg
       61 tagaactgcg cggcccacat ctgatgctgg ccgacctcgg tggtgatgta ggtgtcgcgg
      121 cccttggtca gttcgtacag ccgctcgatg gcgtactgcg gcttgatggt ggtttccgag
      181 gtccggtact tcaggcactg gcgcgcgcgc cacgtatcga tctgggccca ccagtcggtc
      241 agggcggcct tgttgggctg atggccggcg gccttccacg cggcgatcat atcctcgagc
      301 acactggcgg cgtcgccgat gatcggcagg tcgacgcgga cgttcttgtt gatcgacgag
      361 gcgtcgatat cgatgtggat cttcttgctg cccggcgaga aggcgtccag ccggccggtg
      421 acgcggtcgt cgaagcgggc gccgacgcag atcatcacgt cgcagtcatg catggcgtta
      481 ttggcttcga acgtgccgtg catgcccagc atgcccagcc aggccggatc ggcggccggg
      541 aaggcgccca ggcccatcag ggtcgaggtg accggcgcgc cggtcagggc ctggaactcg
      601 cgcaaggccg cgctggcctt ggggccggcg ttgatgacgc cgccgccggt atagaagatt
      661 ggccggcggg cgcgggcgat catcgccgcc gcctcggcga tgcggtttgg ctcgcccttg
      721 gtcctggggt tgtaggcgtg ggtcgaggcc acttccgtcg ggccgtaata ttcgcccttg
      781 gcgaactgca cgtccttggg gatgtcgatc agcaccgggc cggggcggcc ggtggtcgcg
      841 atcttgaacg cctcatggat gatctgcggc aggtcgcgga cgtctttgac cagatagttg
      901 tgcttggtgc acgagcgggt catgccgacg gtgtcggcct cctggaaggc gtcggtgccg
      961 atcaggtggg tcgggacctg gccggtgatg acgaccatcg ggatggagtc catcagggcg
     1021 tccatgatgc cagtgatggc gttggtcgcg cccgggcccg aggtgaccag cacgacgccc
     1081 ggcttgcccg agctgcgcgc atagccttct gcggcgtggg tcgcgccctg ctcgtggcga
     1141 accaggatgt gctgcaggcg cggctcgtgg aacagcgcgt catagatcgg cagcaccgcg
     1201 ccgcccggat agccgaacag cacttcgacg ccctggtcca cgaggccgcg aaccacgatc
     1261 tcggcgccgg tcatggcgcg gtcgatggtt tcgctgggag ccgggctctc gatggtttga
     1321 tgggcggtca tggcaaaggt ccgaaagggg gtggcggtcc gtaggggggc ggagaagacg
     1381 gaggctgaaa agaaaaaagg tcccgaagga cctttgcgcg cgcgactgaa ctacgacgtg
     1441 acttcgaggt cacatcgcgg acagtcgctc cataagtacg atcatggttt tgcgcacgag
     1501 tcttgctcca aagctacgga aagggtgatg ccatgcgcga aagggggcgt caaggtgggt
     1561 ttcaaggcat ggatgtgcct tattgtccca atttggcgtc cagcgtgtcg agcagcagct
     1621 gcagggccgc cccggcgaag gcctgcatgt tggcgggccg gtcattgacg cccgtttcg
//
LOCUS       DAOMEO010000789         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7384_length_1676_cov_2.756940, whole genome shotgun sequence.
ACCESSION   DAOMEO010000789 DAOMEO010000000
VERSION     DAOMEO010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_1676_cov_2.756940"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>289
                     /locus_tag="PLH31_15400"
     CDS             <1..>289
                     /locus_tag="PLH31_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90613.1"
                     /translation="ALSGIAAETLEGGSGIASRTIASLEHQWAQGRELLDARDVLVID
                     EAGMIGSRQMERLLSAAEKAGVKVVLVGDPEQLQAIEAGAAFRSIAERHSHV"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <300..>1676
                     /locus_tag="PLH31_15405"
     CDS             <300..>1676
                     /locus_tag="PLH31_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285209.1"
                     /note="MobA/MobL family protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ti-type conjugative transfer relaxase TraA"
                     /protein_id="HQR90614.1"
                     /translation="TQVRRQHEDWQRDATRQLATGRTGEALAAYEARGMVHAVQTRDQ
                     AREALVERWDRDRVAEPGKSRIILTHTNDEVRDLNLTARDRLRQAGALGEDVTVKAER
                     GERAFAVGDRLMFLKNDRGLGGAGGVKNGMLGEIEQVSASQMTVRLDVGRSVVFDLKD
                     YAQVDHGYAATIHKSQGVTVDRTHVLATPGMDRHGAYVALSRHRDSVHLHYGRDEFED
                     LGKLTRVLSRERAKDMASDFAERRDIHVPRSMRPKESPQRERGMFAGFRPSSPARQPA
                     GPARDEDQLVQRRAIERHARAAADVMRMNDRGLPVLAHQRQALEHAREAVGKIGPHAA
                     KDLERAYVRDPALAGETASGRTQRAIRVLQLEAEVRADPRLRADRFVERWQGLERQRS
                     ALHQAGDVTGAGQVRDRMGAMAKSLARDPQVDSLLRARRPELGLPAEIGRSVGQGLSD
                     YLGIGRGR"
BASE COUNT          251 a          533 c          640 g          242 t
ORIGIN      
        1 gcgctgtcgg gcatcgccgc cgagaccctg gagggcggat cgggcatcgc ctcgcggacg
       61 atcgccagcc tggagcatca gtgggcgcag ggccgcgagt tgctggacgc gcgagacgtg
      121 ctggtgatcg acgaggccgg gatgatcggt tcgcggcaga tggaacggct gctgtcagcc
      181 gccgagaagg ccggggtcaa ggtggtgttg gttggcgatc ccgagcagct tcaggccatc
      241 gaggccggag cggcgttccg gtcgatcgcc gagcgtcata gccacgtcgn nnnnnnnnnt
      301 cacccaggtt cgccggcagc atgaggactg gcagcgcgac gccacgcggc agttggccac
      361 ggggcggacg ggcgaggcgt tggccgctta cgaggcgcgc ggcatggtcc atgccgtgca
      421 gacccgcgac caggcgcgcg aggctctggt cgagcgctgg gatcgcgacc gggtggccga
      481 gccgggcaag agccggatca tcctgacgca caccaatgac gaggtgcgcg atctcaatct
      541 gacggcgcgg gatcggctgc gtcaggccgg tgccttgggc gaggacgtca ccgtcaaggc
      601 cgagcgcggc gagcgcgcgt tcgcggttgg cgatcggctg atgttcctga agaacgatcg
      661 tgggctgggc ggcgccgggg gcgtgaagaa cggcatgctc ggtgagatcg agcaggtgtc
      721 ggccagtcag atgactgtgc gtctcgacgt cgggcgctcg gtcgtcttcg acctgaagga
      781 ctacgcccag gtcgatcacg gctatgccgc caccatccat aagagccagg gcgtcacggt
      841 tgatcggacc catgtgttgg cgacgccggg catggatcgc cacggcgcct atgtcgccct
      901 gtcacgacat cgcgacagcg tgcatctcca ctacggccgc gacgagttcg aggatctggg
      961 caagctgacc cgcgtgctgt cgcgcgagcg ggccaaggac atggccagcg acttcgccga
     1021 gcggcgcgac attcatgtgc cgcggtcgat gcggcccaag gagtcgccgc agcgcgaacg
     1081 cggcatgttt gccgggttca ggccgagcag tccggcgcgt cagccggcgg ggccggcgcg
     1141 cgatgaagac cagcttgtgc agcgtcgggc gatcgagcgc catgcgcgcg ccgcggccga
     1201 cgtcatgcgc atgaacgacc gcggcttgcc ggtgctcgcc catcagcggc aggcactgga
     1261 gcatgcacgc gaggccgtcg ggaagatcgg tccgcacgcg gccaaggatc tggagcgggc
     1321 ctatgtgcgc gatccggccc tggccggcga gacggcctcg ggccgcacgc agcgcgcgat
     1381 ccgcgtcctt cagcttgaag ccgaggtccg cgccgatccg cgtttgcgcg ccgaccgctt
     1441 tgtcgaacgc tggcagggtc tggagcgtca gcgctcggcc cttcaccagg cgggtgacgt
     1501 gaccggcgcg ggccaggtca gggaccgcat gggcgcgatg gccaagagcc tggcgcgcga
     1561 tccgcaggtg gactcgctgc tgcgcgcgcg ccggcccgag cttgggctgc ccgccgagat
     1621 cggccgcagc gtcgggcagg gcctgtctga ctacctcggc atcggtcgtg gccggg
//
LOCUS       DAOMEO010000790         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7405_length_1673_cov_2.423362, whole genome shotgun sequence.
ACCESSION   DAOMEO010000790 DAOMEO010000000
VERSION     DAOMEO010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_1673_cov_2.423362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLH31_15410"
     CDS             complement(<1..1106)
                     /locus_tag="PLH31_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90615.1"
                     /translation="MAKTDSSGAKDGVVVGRKDGLVIGAASVGTVFEWYDFYLYGSLA
                     TYITHHFFSGVNETTGYIFALLAFAAGFAVRPFGALVFGRLGDLWGRKNTFLVTMLLM
                     GLSTFVVGLLPSYDQIGIIAPIALVLMRLLQGLALGGEYGGAATYVAEHAPANRRGLY
                     TSFIQITATFGLFLSLIVILATRNLIGEDAFKEFGWRIPFLISVLLLGVSLWIRLQLS
                     ESPSFQKMIDEGKGSKKPLADSFAKWGNLKIVILALVGLTAGQAVVWYTGQFYALFFL
                     EKMLKVDGGLTNMLVAISLLIGTPFFVFFGWLSDKIGRKWIIMAGCVLACLTYFPLFK
                     TLTVAANPQLAAAVASAPVTVVADPADCSFQFDP"
     gene            1328..1606
                     /locus_tag="PLH31_15415"
     CDS             1328..1606
                     /locus_tag="PLH31_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR90616.1"
                     /translation="MNVSDLVDAAVAADDKLTKAQAKAIIDGVFKAVTDAAVKGEEVS
                     IPGFGKFKVQAKPARTGRNPATGAAIEIAASKKVAFTPAKQLKDAVNG"
BASE COUNT          360 a          518 c          514 g          281 t
ORIGIN      
        1 atcggatcga actggaacga gcagtcggcc gggtcggcga ccacggtcac cggcgcgctg
       61 gcaacggcgg cggccagttg cggattggcc gccacggtca gggtcttgaa cagcgggaaa
      121 taggtcaggc aggccaggac gcagccggcc ataatgatcc acttgcggcc gatcttgtca
      181 gaaagccagc cgaagaacac gaagaacggc gtgccgatca gcagcgagat cgccaccagc
      241 atgttggtca ggccgccatc aaccttgagc atcttctcca ggaagaacag ggcgtagaac
      301 tggccggtgt accagaccac ggcctgaccg gcggtcagac cgaccagagc caggatgaca
      361 atcttcaggt tgccccactt ggcgaagctg tcggccaacg gcttcttgga gcccttgccc
      421 tcgtcgatca tcttctggaa tgacgggctt tccgacagtt gcaggcgaat ccacagcgac
      481 acgcccagca gcagcacgga gatcaggaag ggaatgcgcc agccaaattc cttgaaggcg
      541 tcctcaccga tcagattgcg cgtcgccagg atgacgatca ggctgaggaa caggccgaag
      601 gtcgcggtga tctggatgaa gctggtgtag aggccgcggc ggttggccgg cgcatgttcg
      661 gccacatagg tcgctgcgcc gccgtactcg ccgcccaggg ccaggccctg cagcagtcgc
      721 atcagcacca gggcgatagg cgcgatgatg ccgatctggt cgtagctggg cagcaggccg
      781 accacgaagg tcgatagccc catcagcagc atggtgacca agaaggtgtt cttgcggccc
      841 cacaggtcgc ccaggcgacc aaagaccagc gctccgaacg ggcgcacggc aaagccagcg
      901 gcaaacgcca gcagggcgaa gatatagccc gtcgtctcgt tcacgccgga gaagaaatgg
      961 tgggtgatgt aggtggccag cgaaccgtac aggtagaagt cgtaccactc gaaaaccgtg
     1021 cccaccgagg ccgcgccgat gacgagtccg tccttgcggc ccaccacaac accgtctttc
     1081 gcgcccgagc tatcggtctt cgccatggat tcccccattt ttgttatcgc taccatggca
     1141 tgagttgagc gtcaccacgc tcagactcgc cctcatcggc atgctggcag agtttttggc
     1201 cgccagggag gtgaaccttg ccctttacgt cgtagaaacg cccgtatttg ctgggatgac
     1261 gtgacaaaac cttcccggtt cgtcaggctt ggcgttcctt gattccaact ttggatgagc
     1321 gaagaaaatg aacgtttccg atctggtcga cgccgcagtc gccgctgacg acaagctgac
     1381 caaggctcag gccaaggcga tcatcgatgg cgtgttcaag gccgtgaccg acgccgccgt
     1441 aaagggcgaa gaagtctcga tcccgggctt tggcaagttc aaggtccagg ccaagccggc
     1501 ccgcaccggc cgtaacccgg ccacgggcgc ggcgatcgaa atcgcggcct ccaagaaggt
     1561 cgccttcacg cccgccaagc aactcaagga cgccgtcaac ggctaatcct ctaggatttg
     1621 aagcgaccgg cgagcgcaag cccgccggtc gttttctttt cccgctatct gca
//
LOCUS       DAOMEO010000791         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7418_length_1670_cov_2.321362, whole genome shotgun sequence.
ACCESSION   DAOMEO010000791 DAOMEO010000000
VERSION     DAOMEO010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7418_length_1670_cov_2.321362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..551
                     /locus_tag="PLH31_15420"
     CDS             51..551
                     /locus_tag="PLH31_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR90617.1"
                     /translation="MSGTPSTRLGRQARLQSQTLKMLRKERRMTAAEVASSMGVALRT
                     YQDFEAGRGDLDLAKVRLFAAATRTDAAAITLGLLFEDADIARQTMDNKIPTTFWISL
                     REFRAEAGEQLNTVPPALFLEGFRRVYEEVRAFLRKRADSTDNWLERAIAKTYRSSKD
                     DDDQDA"
     gene            complement(548..895)
                     /locus_tag="PLH31_15425"
     CDS             complement(548..895)
                     /locus_tag="PLH31_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HQR90618.1"
                     /translation="MTTIGAFTQDGEAYVGQLSTLSQEASLRLVPAQKSGERQPDYRL
                     LKMLGEGAGYLDCGAGWKVDDDSSGALVNVKIDDPAWAAPIHARLMRGEDHVLPLVWI
                     RRADPPASPPTSG"
     gene            1263..1649
                     /locus_tag="PLH31_15430"
     CDS             1263..1649
                     /locus_tag="PLH31_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90619.1"
                     /translation="MSEDVELYGVAFMSLYQHDGRFVRGGGVFAFARRDLDGGRTILH
                     LEPVADISRAADSTHPRWDWAVEHGMNELLVHLAGSQQTPGELAPGVGSPPIRWQLHP
                     DELAAEGGPIDERDALADQARLYASG"
BASE COUNT          269 a          563 c          567 g          271 t
ORIGIN      
        1 cgcgtcgcct ataagcgatt ttaggttgtt ttcgtggtct ggagcgtcgc atgtccggca
       61 cgccctctac gcgcctcggg cgccaggctc gcttgcagtc tcagacgctg aagatgctgc
      121 gcaaggagcg ccgcatgacg gcggcggagg tcgccagcag catgggcgtg gccttgcgca
      181 cctatcagga tttcgaggcc ggacgcggcg atctggacct ggccaaggtc cggctgtttg
      241 cagccgccac gcgtaccgat gctgcggcca ttaccctggg cctgttgttc gaagacgccg
      301 acatcgcgcg ccagaccatg gacaacaaga tcccgacgac gttctggatt tcgctacgag
      361 agtttcgggc ggaagccgga gagcagctca atacggtccc ccccgcattg ttcctggaag
      421 ggttccggcg cgtctacgag gaggttcggg ccttcctgcg caaacgcgcc gacagcaccg
      481 acaactggtt ggaacgggcg atcgccaaga cctaccggtc gtcgaaggat gacgacgacc
      541 aagacgccta gcctgaggtt ggcggcgagg ccggcgggtc ggcgcgccgg atccagacca
      601 ggggcaggac atggtcctcg ccccgcatca gccgcgcatg gatcggggcc gcccaggctg
      661 gatcgtcgat cttgacgttg acgagcgcgc cgctgctgtc gtcgtcgacc ttccagccgg
      721 cgccgcagtc gagatagcct gccccctcgc ccaacatctt cagcaggcgg tagtcgggct
      781 gccgctcgcc ggacttttgc gcggggacaa gacgtaggct ggcctcctgg gagagggtcg
      841 aaagctggcc gacataggcc tcgccgtcct gggtgaaagc gccgatggtg gtcatggcag
      901 gtctcctggc cgcccgccgc tgaagggcgg cggcggctca aagctcgggc gaccttaagc
      961 gccaaaccca gctcgaaccg ccgcctccgc cggtcgccga gggcggcgac cggcggtttg
     1021 acgggaggcg aagcgacgct tggcgggcgg ggtgaaagcg tcttcgcggg tcccgttcga
     1081 cggttgtttc ccaaggcggg acgggcctca aggggccgag ccggccggtc gcgtagtgac
     1141 aggccctggc gtggaatcgg cgaatccgaa aggctcccac ccggacggat tcgactcccg
     1201 gcgcaaggcg tgttcttatt atgttcccat cgaccaccgc caacccctcg ccgaggcgcc
     1261 ccatgtccga agacgtcgaa ctctatggcg tggccttcat gagcctctat cagcatgacg
     1321 gccggttcgt gcgcggcggc ggcgtcttcg ccttcgcccg ccgcgatctg gacggcggcc
     1381 gcaccatcct gcacctggag ccggtcgccg acatcagccg cgccgccgac agcacccatc
     1441 cccgttggga ctgggccgtc gagcacggca tgaacgaact gctcgttcat ctggccggct
     1501 cgcaacagac ccccggcgag cttgcgccgg gggtgggttc gccgccaatc cgctggcagc
     1561 tgcatcccga cgaactggcg gccgagggcg gaccgatcga cgagcgcgac gcgctcgccg
     1621 accaggcccg gctctacgct tcgggctgaa ggtccttcag atagtccacc
//
LOCUS       DAOMEO010000792         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7444_length_1665_cov_2.504348, whole genome shotgun sequence.
ACCESSION   DAOMEO010000792 DAOMEO010000000
VERSION     DAOMEO010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7444_length_1665_cov_2.504348"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLH31_15435"
     CDS             complement(<1..738)
                     /locus_tag="PLH31_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HQR90620.1"
                     /translation="MRHQLAQQERRRQNTLLLSLAVAIVASFAFSDFLTRTPLGIATF
                     WPANALLAVGLMVLNPPRRIALVVVSLVFHLLINFLVGDPAGKALLFTGVDILEAFAV
                     WLVAKRFLGAPPRVRTMRQLALLAALALPITLVAALICASFLALGYGLPFLEVLWEWF
                     MCGALGMAVVLPAVLVLIDAEHRRSFHRSLTERLVLLIAVALLTAVCFHPDGVATPFM
                     IFPLAMLASFRLGARGAAQVSLIVACVA"
     gene            complement(946..>1665)
                     /locus_tag="PLH31_15440"
     CDS             complement(946..>1665)
                     /locus_tag="PLH31_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90621.1"
                     /translation="FTAAGGITARLAVERSDGADELVVEISDTGIGIAVEQLDRLFQR
                     FSQADGSIRRSFGGAGLGLAICKALVTLMDGQIGVRSVAGQGSTFWVRLPAAAAKPMA
                     SAKTSAVPGPVARILLVDDHPMNRELGKALLVLAGCDVQTADDGDEAVRAAAMGDFDL
                     ILMDIHMPRMDGLAASQAIRALSGPASAVPIIALSADVMPQQIERCREAGMVDHVAKP
                     IDRAALYAAINRCLAPDPLAA"
BASE COUNT          333 a          548 c          562 g          222 t
ORIGIN      
        1 cgctacgcag gcgacaatca gcgagacctg cgccgcgccg cgcgcgccca gccggaacga
       61 ggccagcatg gccaggggaa agatcatgaa cggggtcgcc acgccgtccg gatggaagca
      121 gacggctgtc agcagcgcca ccgcaatcag cagcacaagc cgctcggtca gggagcgatg
      181 gaaggaccgg cgatgctcgg cgtcaatcag caccagcacg gcgggcagca ccacggccat
      241 gcccagcgcg ccgcacatga accactccca gaggacctcc aggaacggca ggccatagcc
      301 cagggccagg aagctggcgc agatcagcgc tgccacaagg gtgatcggca aggccagggc
      361 ggccagcagg gccaactgcc gcatggttcg cacgcgcggc ggcgccccca ggaaccgctt
      421 ggccacaagc cagacggcga aggcctcaag gatatcaacg ccggtaaaga gcagcgcctt
      481 ccccgcggga tcgccaacca ggaaattgat cagcagatga aagacgaggc tcaccacgac
      541 cagggcgatg cgtcgcggcg ggttcaggac catgaggccg accgccagaa gggcgttggc
      601 cggccagaag gtcgcaatgc ccagcggtgt gcgtgtcagg aaatcgctga aggcgaagct
      661 ggcgacaata gcgacagcca ggctcagcag cagagtgttc tgccgccgcc gctcctgttg
      721 tgccaactgg tgccgcatga tcgttctggt ccccacggat caatacgctc gcgagtgctc
      781 gcggtagccg cccaatttgt tgctgtggcg ctttgccgca gatggcgcac ataactcaag
      841 gcggccccag atccgcgtcg gcgttgcttg gcggaggcac gacaatgcat aaggcgcagg
      901 gtgtttcaag cttgagcgct gtccgccacg cggggcgcca atcggtcagg cggccaacgg
      961 atcgggcgcg aggcagcgat tgatcgcggc gtacagcgcc gcccggtcaa taggcttggc
     1021 cacatgatcg accatcccgg cttcccggca gcgctcgatc tgctggggca tgacgtcagc
     1081 gctcagggcg atgatcggca ccgcgctggc gggcccggac agggcgcgga tcgcctggct
     1141 ggcggccagt ccgtccatcc ggggcatgtg aatatccatc aggatcaggt cgaagtcgcc
     1201 catggcggcg gcgcgcacgg cttcgtcgcc atcgtcggcc gtctggacat cgcaaccggc
     1261 cagcaccagc agggccttgc ccaactcgcg gttcatcgga tggtcgtcga ccagcagaat
     1321 gcgggcgacg gggccgggaa ccgcagacgt tttcgccgaa gccatcggtt tagccgccgc
     1381 ggcgggcagc cgaacccaga aggttgaacc ctgacccgcg acgctgcgga cgccgatttg
     1441 accgtccatc aaggtgacca gggccttgca gatcgccagg cccaggccgg ccccgccaaa
     1501 gctgcggcga atggagccat cggcctggga gaaacgctgg aacagtcggt cgagctgctc
     1561 aaccgcaatg cctatgccgg tgtccgagat ttcgacaacc agctcgtccg caccatcgga
     1621 ccgttcaacg gcgaggcggg ccgtgatccc gccagcggcg gtaaa
//
LOCUS       DAOMEO010000793         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7445_length_1665_cov_2.071429, whole genome shotgun sequence.
ACCESSION   DAOMEO010000793 DAOMEO010000000
VERSION     DAOMEO010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_1665_cov_2.071429"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..790
                     /locus_tag="PLH31_15445"
     CDS             227..790
                     /locus_tag="PLH31_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90622.1"
                     /translation="MAKTVYQRNQKVWVESVGAWATIERIVPIWAKGFDEPVRVTYDV
                     GLGREFHAHELKPEEKVDAEGAGMVANWRVLRARNKWQQESDCPHHPYPGTYPVVVTD
                     PNDWGGWRTPGAEYDRDPRKIEYQARLISSAPRLHAIARQILELVADHPEDAPPALAA
                     LAQQASAIERYLHEVPAAESATGRVEG"
     gene            complement(994..1647)
                     /locus_tag="PLH31_15450"
     CDS             complement(994..1647)
                     /locus_tag="PLH31_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HQR90623.1"
                     /translation="MTIATRLALTCAASLLAVSAVQAQEAVKPKAKGDLVLNVRVTDV
                     APDAGNSIVTAAGVASGLKADVNDDIKPTIGLSYFLSDKLAVEVIAGTTQHTVKAVGP
                     GTDIEVHKTWVLPPVVSLQYHPFPAAKFSPYVGAGLNYMLFYSSKDKNGFKVKLDDGF
                     GYALQVGADVPLNARYNLNIDVKKVWFETDASINGGALKSKVKLDPIVASVGVGYRF"
BASE COUNT          308 a          533 c          546 g          278 t
ORIGIN      
        1 cggccggaac gcagcatccc tcggggaagt tgcgcgcctc gtccttcaga aatttctggt
       61 cgtcggaaaa gtcaaaatcc actggggcct ccctggacgg ctcacgggtc ggcgcgtctc
      121 aaggcccacc ctgaacgacc ttgagccgca gggaaagatt gacccggcgt caaggatttc
      181 aggttcttat ggcgctcgtt cagttcacgt cgcgtccaag gggcccatgg ccaagaccgt
      241 ctatcagcgc aaccagaagg tctgggtcga gagcgtcggc gcctgggcga cgatcgagcg
      301 catcgtgccc atctgggcca agggcttcga tgagcccgta cgcgtcacct atgacgtcgg
      361 cctgggtcgc gaattccacg cccatgagct gaagcccgag gaaaaggtcg acgccgaggg
      421 ggctggcatg gtcgccaact ggcgggtgct gcgggcccga aacaagtggc aacaggaaag
      481 cgactgcccg caccacccct atcccggcac ctatccggtg gtggtcaccg atcccaatga
      541 ctggggcggc tggcgcacgc cgggggcgga atatgatcgc gacccgcgca agatcgaata
      601 ccaggcccgg ctgatctcca gcgcgccgcg tttgcacgcc atcgcccggc aaattctgga
      661 gctggtcgcc gaccatcccg aagatgcgcc gcccgccctc gccgccctcg cccagcaggc
      721 gtcggcgatc gagcgctatc tgcatgaggt tccggcagcc gaaagcgcga cgggtcgggt
      781 cgaaggctaa cgccccatga caggcgggcg cggttcggct gatcaggaat gaccagaacc
      841 cgggtcggac ggcatcaggc tcgacactga tgccgtccgg cctcgcccgg ggcgaggcaa
      901 acgacgctcc ggcctcaacc gccggcgctc cgggcacagt ttccgcagcc cgcgccggga
      961 cggcgcgacg ctgcgaaacg tctcaggtgg gcgttagaac cgataaccga cgccgaccga
     1021 agccacgatg gggtccagct tgaccttcga cttcagcgcg ccgccattga tcgaggcgtc
     1081 ggtctcgaac cacaccttct tgacgtcgat gttcagattg tagcgcgcgt tcagcggcac
     1141 gtcagcgccg acctgcaggg cgtaaccgaa gccgtcatcc agcttcacct tgaagccgtt
     1201 cttgtccttg ctgctgtaga acagcatgta gttcaggccc gcgccgacat aggggctgaa
     1261 cttggcggcc gggaacgggt gatactgaag cgacacgacg ggcggcagga cccaggtctt
     1321 gtgaacctcg atgtcggtcc cgggcccgac ggccttgacg gtgtgctggg tggtgccggc
     1381 gataacctcg acggcgagct tgtcgctcag gaaataggac aggccgatgg tcggcttgat
     1441 gtcgtcattg acgtcggcct tcaggcccga ggcgacgccc gcagcggtga caatcgaatt
     1501 gccggcgtca ggcgctacat cggtgacgcg gacgttcaga accagatcgc ccttggcctt
     1561 gggcttgacg gcctcttggg cttgaacggc ggaaacggcc agaaggctgg cggcgcaagt
     1621 cagagccagg cgggtcgcga tggtcatctt ttatccccat atttc
//
LOCUS       DAOMEO010000794         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7446_length_1665_cov_1.698758, whole genome shotgun sequence.
ACCESSION   DAOMEO010000794 DAOMEO010000000
VERSION     DAOMEO010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7446_length_1665_cov_1.698758"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLH31_15455"
     CDS             complement(<1..380)
                     /locus_tag="PLH31_15455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR90624.1"
                     /translation="MIDLRDLRDEDEGVLFQWRSEPEVDRWMSDAAFPSREAHAAWFE
                     ILRTDPDMRGWLITRGGGAAGLLTLSGLQSHHRRASWNWFLGSAEARGRGVGRAAQVL
                     GLDRAFGELGLHKVFAEVMADNDAA"
     gene            complement(469..1302)
                     /locus_tag="PLH31_15460"
     CDS             complement(469..1302)
                     /locus_tag="PLH31_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQR90625.1"
                     /translation="MTLSVNTNQPALIALQNLNKTNDDMQGVQTRINTGLIVSSAKDN
                     AAVWSIAQEQRADMNALSAVKMSLDRATSVADVALAAGESVSDLLGLMREKVVAAKDT
                     SLTTKSRQALNSDFQGLIKNLNQVLSSATFDGANLLNGSLTADVSFLADADAGAAITL
                     NLQNLSLGGTINTLTATDDILDVANATNVLARLDATMGAVNQAVGNIGTQAKQIEAHH
                     SFVSKLSDVLETGVGNLVDADLAKESARLQALQVKQQLGAQALSIANSAPQIILKLFN
                     G"
     gene            complement(1479..>1665)
                     /locus_tag="PLH31_15465"
     CDS             complement(1479..>1665)
                     /locus_tag="PLH31_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90626.1"
                     /translation="SEPQPGDLRLVIEQDEGSGAFIYKTVDRRTGETLQSLPREDVLK
                     LKHAATYDPGAVYDGLS"
BASE COUNT          326 a          517 c          501 g          321 t
ORIGIN      
        1 gcagcgtcat tgtccgccat cacctcggca aacactttgt ggaggccaag ctctccgaaa
       61 gcgcgatcaa gaccaagaac ctgggcggcg cgcccgaccc cccgcccgcg ggcctctgcg
      121 gaaccaagaa accagttcca gcttgctcgc cgatgatggc tttgaagtcc cgagagcgtc
      181 aggagtcccg ccgccccccc tccccgcgtg atcagccagc cgcgcatgtc gggatccgtg
      241 cgaaggattt caaaccacgc agcgtgggct tcacggctgg ggaaagcggc atccgacatc
      301 caccgatcaa cttcgggttc cgagcgccac tgaaacagca cgccctcatc ctcgtctcgc
      361 aaatcgcgaa ggtcgatcat tacggccgat tcccctcagg caagaccaaa gcgtacctcg
      421 cgcgctagcg aaggcctaga gtgctggcgc tcaggcgaag cctatggact agccgttgaa
      481 caacttcaga atgatctgcg gggccgagtt ggcgatggac agggcctggg cgcccaattg
      541 ctgcttcacc tgcagggcct gcaggcgcgc gctttccttg gccaggtcgg cgtcaaccag
      601 gtttccgacg ccggtctcca gcacatcaga cagtttggac acgaagctgt ggtgagcctc
      661 gatctgcttt gcctgggtgc cgatattgcc gacggcctgg ttgaccgcgc ccatggtggc
      721 gtcaaggcgc gccaggacgt tggtcgcgtt ggcgacgtcc aggatatcat cggtagcggt
      781 cagggtgttg atcgtgccgc ccagcgacag attttgcaga ttgagcgtga tcgccgcgcc
      841 ggcgtcggca tcggccagga acgagacgtc ggcggtcagg ctgccattaa gcagattggc
      901 tccgtcgaac gtggcgctgg agagcacctg gttcaggttc ttgatcaatc cctggaaatc
      961 ggagttcaac gcctggcggg acttcgttgt cagcgaagta tccttcgcgg cgacgacctt
     1021 ctcgcgcatg agaccgagca ggtccgaaac cgattcgccc gcagccaagg ccacatcggc
     1081 aaccgatgtc gcgcgatcca ggctcatctt gaccgccgaa agcgcgttca tgtccgcgcg
     1141 ctgttcctga gcaatagacc agaccgccgc attatccttg gcgctggaaa ctatcagacc
     1201 cgtgttgatg cgggtttgca caccctgcat gtcatcattg gtcttgttca ggttctgcag
     1261 agcaatcagc gcgggctgat tggtgttgac gctaagcgtc attcgcggcc tccgatcgga
     1321 aattttttcc gggtttggtt cgtgcgcctc tcagcgaacg agccctcacg gaactgaccg
     1381 taggcacaga aagtaaggtt atggttaacg gaaataaacc ataaccagcc caccccgcga
     1441 ctccgcgtcg cagggggtga aaagcgcgct taagcgcgtc agctcagacc gtcgtaaacc
     1501 gcgcccggat catacgtagc ggcgtgtttg agcttgagaa cgtcttcccg aggcagactc
     1561 tgaagcgttt cgcccgtgcg gcggtcaacg gtcttgtaga tgaaggcgcc gctgccctcg
     1621 tcctgctcga tgaccagacg aagatcgccc ggctgaggct cggac
//
LOCUS       DAOMEO010000795         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7447_length_1664_cov_2.590429, whole genome shotgun sequence.
ACCESSION   DAOMEO010000795 DAOMEO010000000
VERSION     DAOMEO010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_1664_cov_2.590429"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLH31_15470"
     CDS             complement(<1..789)
                     /locus_tag="PLH31_15470"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517325.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQR90627.1"
                     /translation="MRRVLIGLARTGWLKAALIVGGLTVAGVAVATAQGPAPAAGVQK
                     VRFGGDRAETRVVIDLDRAAVGRLIADGALDQRIVIGLPNVSVSGDLQGAGQGLVKRW
                     VIDEAGGGARLRLDLSGRAEVRRRFLLPPGDGATAYRYVIDLKATDGAKPASAPKLTL
                     VSSPVKAAPLRLKKVIVIDAGHGGKDPGSLGASVYEKDVTLAAAKALKTRLEKSGRFQ
                     VVMTRETDTFVPLEARVQIARRADADLFLARLLRILLMENQMRRV"
     gene            1198..1416
                     /locus_tag="PLH31_15475"
     CDS             1198..1416
                     /locus_tag="PLH31_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90628.1"
                     /translation="MGCAAPVRLWHPTSRNRTGGATDGDARACLSYRRPGPEPAVRRG
                     DSLNVEEDADRRSTRRRDACGRRGRNPG"
     gene            1340..>1664
                     /locus_tag="PLH31_15480"
     CDS             1340..>1664
                     /locus_tag="PLH31_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQR90629.1"
                     /translation="MSKKMLIDAAHAEETRVVVVDGTRVEEFDFESQTRKQLRGNIYL
                     AKVTRVEPSLQAAFIEYGGNRHGFLAFNEIHPDYYQIPVADREALMRDDSHDDEDDHV
                     ATRRGD"
BASE COUNT          320 a          562 c          496 g          286 t
ORIGIN      
        1 cacgcgtcgc atctggtttt ccataagcaa tattctcagc aatcgtgcca aaaataggtc
       61 agcgtccgca cggcgggcga tctgcacgcg cgcttccagc ggaacgaagg tgtctgtctc
      121 gcgagtcatc accacctgga agcggccgga tttctccagt cgggtcttca gggccttggc
      181 agcggccagg gtgacgtcct tttcatagac ggacgcgccc agcgagcccg gatccttgcc
      241 gccgtgaccg gcgtcgatga cgatcacctt cttcaggcgc agcggggcgg ccttaacggg
      301 gctcgatacc agggtcagtt tcggcgcaga tgcgggtttc gcgccatccg tcgccttgag
      361 gtcaatgaca tagcggtagg ctgtcgcacc atcgccgggc ggcagtagga agcgacggcg
      421 cacctcggct cggccggaaa ggtccaggcg caggcgcgcg ccgccgccgg cctcatcgat
      481 gacccagcgc ttgaccaacc cttggccagc gccctgcaga tcacccgaaa cggaaacatt
      541 gggcagtccg atgacaatac gttgatccag agcgccatcg gcgatcagcc ggcctacggc
      601 ggcgcgatcc agatcaatga cgacgcgggt ttcggcgcgg tcgccgccaa accggacttt
      661 ctgcacgcca gccgctggcg cagggccctg cgcagtcgcc accgcgacgc cagccacggt
      721 caagccgccg acgattaacg ccgccttcaa ccagcctgtt cgagctaacc cgatgagaac
      781 tctacgcata cctcgcccaa gcattccgga gctggtgaac caaccgtcaa tatcggccac
      841 ctgcggtagc gaaagcgtta aatcagcccc tcttgtcgag cgctttcctt ttgccccccg
      901 atgttgctac aattccaatg cgtgcgagcg acgtgatcgt catggaagct tgcgcgggcg
      961 atctgcgacg atagaagccc tagcgcctct agccgcccgt aggctgcaga cttggtagtg
     1021 atcaaggacc gatcctttcg tttgcaggcc tgacgcctgc ctcgggagcg gtccgaggag
     1081 cccgcgtcgg gctctccgcc cttccgggcg acgacgcgcc atccgatccg cgccacccgg
     1141 cgcgacccgg taaaccatcc agtccgcgtc ctaggcgtgg tggagaatag aaacctcatg
     1201 ggctgcgccg cacccgtccg cctttggcat ccgacctctc gcaaccgcac tggcggagcg
     1261 acggacggtg acgcgcgcgc ctgtctttca tatcggcgac cgggcccaga gcccgccgtg
     1321 cgccgtggag attcccttaa tgtcgaagaa gatgctgatc gacgcagcac acgccgaaga
     1381 gacgcgtgtg gtcgtcgtgg acggaacccg ggttgaagaa ttcgatttcg aaagccagac
     1441 ccgcaaacaa cttcgcggaa acatctatct cgccaaggta acccgcgttg aacccagcct
     1501 ccaggctgcg ttcatcgagt atggcggcaa ccgccacggt ttcctcgcct tcaacgagat
     1561 ccatcccgac tactaccaga ttccggtcgc cgaccgcgaa gcgctgatgc gcgacgattc
     1621 gcatgacgac gaggacgacc acgtcgccac gcgccggggc gatg
//
LOCUS       DAOMEO010000796         1657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7502_length_1657_cov_2.149813, whole genome shotgun sequence.
ACCESSION   DAOMEO010000796 DAOMEO010000000
VERSION     DAOMEO010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7502_length_1657_cov_2.149813"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLH31_15485"
     CDS             <1..749
                     /locus_tag="PLH31_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQR90630.1"
                     /translation="DQPRRTFREDDLVELSNSIREKGVLQPILVRPAPGAAGEYQIVA
                     GERRWRAAQRAGLRSVPILVRELDDLAVLEIGIIENVQRSDLNILEEALSYRVLMDKF
                     DRTQENIAQTIGKSRSHVANTLRLLALPEEVQQHLVSGELSAGHARAIAAAEDPAALA
                     REIIGKGLSVRDTEALARKTPDARPSKNKGGRPPKVKDTDTQALESDLSSVLGLDVVI
                     DHRDGAGQLTIRYATLEQLDDLCNRLTRGV"
     gene            complement(966..>1657)
                     /locus_tag="PLH31_15490"
     CDS             complement(966..>1657)
                     /locus_tag="PLH31_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQR90631.1"
                     /translation="AGALGRDSTLRKVGEKASGCAVIPCYEDEAGDMARLARETLARD
                     KVSLNSEALDLFVSRLPKERGVARMEIERLILFLGPGSGVNATAADLTDFLGVEPEAS
                     LSDAAVDAFGGRLGAAQAGLRRAAAEGEGGPAAVRAMGYHLGRLRRTLTLHKNGIDLA
                     QAAKASGVFWKQEREFLRQARAWTLELLLDIQGEILNADRACKTSGSPDQLISERLAL
                     MIAGRARRLGL"
BASE COUNT          268 a          574 c          558 g          257 t
ORIGIN      
        1 ccgaccagcc gcgccggacc ttccgcgagg acgacctggt cgagctgtcc aattcgatcc
       61 gcgagaaggg cgtcctgcag ccgatcctgg tgcgacccgc gcccggcgcg gccggggaat
      121 accagatcgt cgccggcgag cgccgctggc gcgcggccca acgggccggc ctgcgcagcg
      181 tgccgatttt ggtgcgcgaa ctggatgacc tggctgtcct cgagatcggc atcatcgaga
      241 acgtccagcg ctccgacctc aatatcctcg aagaggcgct gtcctatcgc gtcctgatgg
      301 acaagttcga ccgcacccag gaaaacatcg cccagaccat cggcaagagc cgcagccacg
      361 tggccaacac tctgcggctg ctggccctac cggaagaggt tcagcaacac ctggttagcg
      421 gggagctgtc ggccggtcat gcccgtgcca tcgccgccgc cgaagacccc gccgccctgg
      481 cgcgcgagat catcggcaag ggcctgtcgg tgcgcgacac cgaggccctg gcccgcaaga
      541 cccccgacgc ccgcccgtcc aagaacaagg gcggccgtcc gcccaaggtc aaggacaccg
      601 acacccaggc cctggagtcc gatctgtcgt cggtgctggg cctggacgtg gtcatcgatc
      661 atcgcgacgg cgccggccag ttgaccatcc gctatgcgac gctcgagcag ctggacgacc
      721 tctgcaaccg gctcacgcgc ggggtctgat cgtccgcacg aaatgtgaag tcaaaaggtg
      781 tgaagtcacg ctttttcgac ggcctctgtc gcgcacggag cggcgccttt ccgtactcag
      841 ggaggcgccg ttttcgtgag acgaaggtcg atttctcgat aatccatgta aggcgctgtt
      901 ccgtaaggct ttccggaaag tcatccacag cggcgcaaac gccccgtttt cggggttttt
      961 ttggcctaaa gccccaaccg gcgtgcgcga ccggcgatca tcagggccag gcgctcggaa
     1021 atcagctggt cgggcgaacc ggaggtcttg caggcgcgat cggcgttgag aatctcgccc
     1081 tggatgtcga gcagcagctc cagggtccag gcgcgggcct gacgcaggaa ctcgcgctcc
     1141 tgtttccaga acacgccgga ggccttggcg gcctgggcca gatcgatgcc gttcttgtgc
     1201 agggtcaggg tgcggcgcag gcggcccagg tgatagccca tggcccgcac cgccgccggt
     1261 ccgccttcgc cttcggccgc cgcgcgacgc aggccggcct gggcggcgcc cagacggccg
     1321 ccgaaggcgt cgacagcggc gtcgctgagc gaggcctcgg gctcgacgcc caggaagtcg
     1381 gtcaggtcgg ccgccgtggc gttgacgccg ctgccggggc ccaggaacag gatcaggcgc
     1441 tcgatctcca tccgcgccac gccgcgctcc ttgggcaggc gtgaaacgaa caggtcgagg
     1501 gcttcgctgt tgaggctgac cttgtcccgc gccagggtct cgcgcgccag gcgggccatg
     1561 tcgccggcct cgtcctcgta gcaggggata acggcgcagc cgctggcctt ctcgccgacc
     1621 ttgcgcaggg tcgagtcgcg gcccagcgcg ccggcct
//
LOCUS       DAOMEO010000797         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7541_length_1649_cov_2.653074, whole genome shotgun sequence.
ACCESSION   DAOMEO010000797 DAOMEO010000000
VERSION     DAOMEO010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7541_length_1649_cov_2.653074"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1177
                     /locus_tag="PLH31_15495"
     CDS             62..1177
                     /locus_tag="PLH31_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90632.1"
                     /translation="MVTTSKPLKALLAMAGGAALLSACASADDVAALRQPAMPPVAIA
                     KAAQHKPFFRNVSLQAVEGAPEFRWFDGGAIITTRPTRVQVVENLSRHLDRADMLARS
                     RLDSEYMLYVRFDELRGPNVWFGTDKLASARVTFRLVRWRTGELVKESAVEASYRAEW
                     TGFTPEMTRAALGGPILASKDAVLAPVGGALKGVALGYYVNQNLVVSIADAPYAGLVG
                     AVEASRIGGVSRAPAGFASAFATAAAIGSASGRFTDLEAMLAGGAITAAGAAAGPTPI
                     ARPISANGEVTTAIAGSARRLAATRGLMDLAFDQFMAELSEDGSVNYKTAVSCAALNG
                     VSRKGPYLRENASSYAVDCPGSTYNESKTQQVYPSRF"
     gene            complement(1183..>1649)
                     /locus_tag="PLH31_15500"
     CDS             complement(1183..>1649)
                     /locus_tag="PLH31_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90633.1"
                     /translation="IELELSRPKGDEDGVLIAHGSMPAGYVLYIDKGRLIYETSLIPW
                     SEVIASPAKLPRGTFKVKYEQTMTSRPFDGRGALYVNGRKVAERKFDRVLFAPGYDGF
                     CVGADYGNRVSRAYEGPNPFQGQIQRVLIDVDTAAMKPLEIMRFMKAMSIRV"
BASE COUNT          259 a          579 c          539 g          272 t
ORIGIN      
        1 gcggcgaaat ggcccattaa ggttgtcagg gcattctcca tcgcgatttt ggagagctcc
       61 gatggttacg acgtccaagc ccctgaaggc gctgctcgcg atggctggcg gggccgccct
      121 gctgagcgcc tgcgccagcg ccgacgacgt cgcggccctg cgccagccgg ccatgccgcc
      181 ggtcgccata gccaaggcgg cccagcacaa gccgttcttc cgcaacgtct cgctgcaggc
      241 ggtcgagggc gcgcctgagt ttcgctggtt cgatggcggc gcgatcatca ccacccgccc
      301 cacccgcgtg caggtggtcg agaacctctc ccgccatctg gaccgcgccg acatgctggc
      361 ccgcagccgg ctggacagcg aatacatgct ctatgttcgc ttcgacgaac tgcgcggccc
      421 caatgtgtgg tttggaaccg acaagctggc ctcggcccgc gtgaccttcc gcctggtgcg
      481 ctggcgcacg ggcgaactgg tcaaggagtc cgccgtcgaa gccagctatc gcgccgagtg
      541 gacgggcttt acgcctgaaa tgacccgcgc ggccctcggc ggacccatac tggcctccaa
      601 ggacgccgtg ctggcgccgg tcggcggcgc gctgaagggc gtggccctgg gctactatgt
      661 gaaccagaac ctggtcgtct cgatcgccga cgccccctat gccggcctgg tcggcgcggt
      721 ggaggcctcg cggatcggcg gtgtcagccg cgcgccggcc gggtttgcct ccgccttcgc
      781 cacggcggcc gctatcggca gcgccagcgg tcgcttcacg gatctggagg ccatgctggc
      841 cggcggggcg atcaccgccg ccggcgcggc tgcaggtcca acgccgatcg ctcgcccgat
      901 ctcggccaat ggcgaggtga ccaccgccat cgccggcagc gcccgccgcc tggccgcaac
      961 gcgcggcctg atggacctgg cttttgacca gttcatggcc gaactcagcg aggacggctc
     1021 ggtcaactac aagacggccg tctcgtgcgc ggccctgaac ggcgtcagcc gcaagggccc
     1081 ctatctgcgc gagaacgcca gcagctatgc ggtcgactgc ccgggctcga cctacaatga
     1141 gagcaagacc cagcaggtct atccgtcgcg cttctaaacg cttcagaccc gaatgctcat
     1201 cgccttcatg aaccgcatga tctccagcgg cttcatggcg gcggtgtcga catcgatcag
     1261 cacgcgctgg atctgaccct ggaaggggtt ggggccctca taggcgcgcg agacccgatt
     1321 gccgtagtcg gcgcccacgc agaagccatc atagcccggc gcgaacagca cccggtcgaa
     1381 cttgcgctcg gccaccttgc ggccattcac atagagcgcg ccgcgcccgt cgaaggggcg
     1441 tgaggtcatg gtctgctcgt acttcacctt gaaggttccg cgcggcagct tcgccggcga
     1501 ggcgatgacc tcgctccagg ggatgaggct ggtctcgtag atcagtcggc ccttgtcgat
     1561 atacaggacg tagcccgccg gcatcgagcc atgcgcgatc agcacgccgt cctcgtcgcc
     1621 cttgggccgt gaaagctcca gctcgattg
//
LOCUS       DAOMEO010000798         1647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7562_length_1647_cov_2.223618, whole genome shotgun sequence.
ACCESSION   DAOMEO010000798 DAOMEO010000000
VERSION     DAOMEO010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_1647_cov_2.223618"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>1241
                     /gene="rodA"
                     /locus_tag="PLH31_15505"
     CDS             140..>1241
                     /gene="rodA"
                     /locus_tag="PLH31_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQR90634.1"
                     /translation="MTLSGGLSRPGERDRPIIKFMEIDWTFCLALCLIAGAGALMLFS
                     IAGASWEPWAAKHLVRFGIYFVLMIVLAMIDLRLWFMIAYPVYVVGLLLLVAVELFGD
                     TSLGAQRWLSIGGFRFQPSEIMKIGLVLALARYYHGLSADSARMSWRLLIPAAMIAAP
                     VLLVAHQPDLGTAVLVASTGLAIVVLAGLSWKIIVAGIISVLVAIPPFIIFVLHEYQR
                     NRIMTFMNPEADPSGNGYHIMQSKIALGSGGLMGKGFGLGSQSQLNFLPEKQTDFIFA
                     TLAEEFGFLGCFSVLFLYGVVIFMALRIASISHSHFGRLAAAGTTATFALYVLINGAM
                     VMGMAPVVGVPMPMLSYGGTVMLTVMIGFGLVQ"
     assembly_gap    1242..1251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1275..>1647)
                     /locus_tag="PLH31_15510"
     CDS             complement(1275..>1647)
                     /locus_tag="PLH31_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQR90635.1"
                     /translation="AWSQWEGDTISLRGPEARPPKFNEEDFAIAFARIECHFFTHRGF
                     FEHDGWILKNIDKIKHIPGWIVQGRFDVVTPLDSAWSLHKAWPQARFDIIWDAGHAST
                     EPGVIDGLVRATEAALVSNSG"
BASE COUNT          240 a          539 c          530 g          328 t
ORIGIN      
        1 aggacccgga aatccgcgcc cgcatcgaaa agccgatgcc catgcccgac gcgccgcccg
       61 acgcgttcat cgagggcgcg gcgccggaag atccgatcgt cggcgcgccg ccacccggca
      121 cgccgcagga gccgcgcata tgaccctcag cggcggcctc agccggcccg gcgaacgcga
      181 ccggccgatc atcaagttca tggagatcga ctggaccttc tgtctggccc tctgcctgat
      241 cgcgggcgct ggcgcgctga tgctgttctc gatcgcaggc gcgtcctggg agccgtgggc
      301 cgccaagcat ctggtgcggt tcgggatcta tttcgtgctg atgatcgtgc tggccatgat
      361 cgacctgcgc ctgtggttca tgatcgccta tcccgtctat gtggtgggac tgttgctgct
      421 ggtcgccgtg gaactgttcg gcgacacctc cctgggcgcc cagcgctggc tgtcgatcgg
      481 cggcttccgc ttccagccct cggaaatcat gaagatcggc ctcgtcctgg ccctggcgcg
      541 ctactatcac ggcctctcag ctgacagcgc ccggatgtcg tggcggctgc tgatcccggc
      601 ggcgatgatc gccgcgccgg tgctgctggt ggctcaccag cctgaccttg gcaccgccgt
      661 gctggtcgcc tccacgggcc tggccattgt ggtgctggcc ggcctgtcct ggaagatcat
      721 cgtagcgggg atcatcagcg tgctggtcgc catcccgccc ttcatcattt tcgtgctgca
      781 cgaatatcag cgcaaccgca tcatgacctt catgaacccc gaggccgatc cgtccggcaa
      841 cggctatcac atcatgcagt ccaagatcgc cctgggttcg ggcgggctga tgggcaaggg
      901 ctttggtctt ggctcgcaga gccagctgaa cttcctgccc gagaagcaga ccgacttcat
      961 cttcgccacc ctcgccgagg agttcgggtt cctgggctgt ttcagcgtgc tgttcctgta
     1021 tggcgtggtg atcttcatgg ccctgcgcat cgcctcgatc agccacagcc atttcggacg
     1081 cctggccgcc gccgggacca ccgccacctt cgcgctctat gtgctgatca atggcgccat
     1141 ggtcatgggc atggcgccgg tggtcggcgt gccgatgccg atgctgtcgt atggcggcac
     1201 cgtgatgctg acggtgatga tcggctttgg cctggtccag gnnnnnnnnn nagggctcgc
     1261 tgatgtagca gcggtcagcc gctgttggac accagtgccg cctcggttgc gcgcaccagc
     1321 ccgtcgatga cgccaggctc ggtcgaggcg tggcccgcgt cccagatgat gtcgaagcgc
     1381 gcctgcggcc aggccttgtg caggctccag gcgctatcca gcggcgtgac cacatcgaaa
     1441 cggccctgca cgatccagcc ggggatgtgc ttgatcttgt cgatgttttt caggatccag
     1501 ccgtcgtgct cgaagaagcc gcggtgggtg aagaagtggc actcgatccg cgcgaaggcg
     1561 atggcgaagt cttcttcatt gaacttaggg ggacgcgcct cgggccctcg cagcgaaatg
     1621 gtgtcgcctt cccactggct ccaggcg
//
LOCUS       DAOMEO010000799         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7571_length_1645_cov_2.017610, whole genome shotgun sequence.
ACCESSION   DAOMEO010000799 DAOMEO010000000
VERSION     DAOMEO010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_1645_cov_2.017610"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..897)
                     /locus_tag="PLH31_15515"
     CDS             complement(34..897)
                     /locus_tag="PLH31_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HQR90636.1"
                     /translation="MSRFAYVNGRFVRHGEAAVHIEDRGYQLADGVYEVWAVFEGKLA
                     DAEGHFARLWRSLDELRIAHPMGQAALTFVLREAVRRNRVRNGLVYLQVTRGVARRDH
                     AFPNPAVPPSMVVTAKSTDPNLAERKAAQGAAVISMPENRWGRCDIKSVGLLPNALAK
                     QAARERGAIEAWFVDDLGLVTEGASSNAWIVDAEGHLRTRDTQANILRGVTRSTLFEV
                     IREAGLPINEKPFTIAEAQSAREAFVTGAGTLVMPIISVDGVKVGDGSAGPVATRLRR
                     LYIERAREKAI"
     gene            complement(898..>1645)
                     /locus_tag="PLH31_15520"
     CDS             complement(898..>1645)
                     /locus_tag="PLH31_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQR90637.1"
                     /translation="ALSVSMETSEQVFERAHGGTLYLDEVADMPRETQSRILRVLVEQ
                     RFRRVGGDNDVQVDVRVISSTSRDLRDEIAGGRFREDLFHRLNVVPVRVPGLSERRED
                     IPELISFFIDRIVEATGLPRRRLGDDALATLQVQTWPGNVRQLRNNVERMLILAAGEP
                     GELITAEMLAGAEQPSAGHAGAIGAERIIALPLREARELFEREYLNAQILRFGGNISR
                     TANFIGMERSALHRKLKSLGVAGARTDEED"
BASE COUNT          289 a          570 c          499 g          287 t
ORIGIN      
        1 gctgtttccg accagccgtt ttcggccaat ccgtcagatc gctttctcac gcgcccgctc
       61 gatatagaga cgccggagcc gcgtcgccac cgggccagcg gagccatcgc caactttcac
      121 gccgtcgacg ctaatgatcg gcatcaccag cgttccggcg cccgtgacga acgcttcccg
      181 ggcgctctga gcctcggcga tcgtgaaggg cttctcgtta atcggcagcc ccgcttcgcg
      241 gatcacctca aacagggtgg atcgggtcac cccgcgcagg atgttggcct gcgtgtcgcg
      301 ggtgcgcaga tgaccttcag cgtcaacaat ccaggcgttg gatgacgcgc cttccgtcac
      361 cagtcccagg tcatcgacga accaggcctc gatcgctccg cgctcgcgcg ctgcctgctt
      421 ggccagagca ttgggcagca gaccgaccga cttgatgtcg caacgccccc agcggttctc
      481 cggcatgctg atcacggccg cgccctgcgc cgccttgcgc tcggcaagat tggggtctgt
      541 cgacttggcc gtgaccacca tcgacggcgg aacggcggga ttggggaagg cgtggtcacg
      601 acgggcgacg ccgcgcgtca cctgcagata gaccaggcca ttacgcaccc ggttgcgccg
      661 aaccgcctct ctcaacacga aggtcagggc cgcctgcccc atcggatgag cgatccgtag
      721 ctcgtccagg ctgcgccaaa gccgggcgaa gtgcccctcg gcgtcggcca gcttgccttc
      781 gaatacggcc cagacttcat aaaccccgtc cgccagctga tagccgcggt cctcgatatg
      841 gacggcggct tcgccgtgcc gaacgaagcg gccgttcaca taggcaaagc gcgacatcta
      901 gtcttcctcg tcggtccggg cgccggcgac gcccagggac ttgagcttgc ggtgcagcgc
      961 cgagcgctcc atgccgatga agttggcggt gcgcgaaata ttgccgccga accgcaggat
     1021 ctgggcgttc agatactcgc gctcgaacag ctcacgggct tcgcgaagcg gcagcgcaat
     1081 gatcctctcg gccccgatcg cgcctgcatg gccagccgac ggttgctcgg cgcccgccag
     1141 catctcggcg gtgatcaatt cgcccggttc gccggccgcc aggatcagca tgcgttcaac
     1201 attgttccgt agctgccgga cgttgccggg ccaagtctgg acctgtagcg tcgccagcgc
     1261 gtcgtcgcca agccttcgcc gcggcaagcc ggtcgcctcg acaattcggt cgatgaaaaa
     1321 gctgatcagc tcgggaatgt cttctcgacg ctcagaaaga ccgggcaccc tcacgggcac
     1381 aacgttcaga cgatgaaaca ggtcttcccg gaagcgcccc ccagcgattt cgtcacgcag
     1441 atcacgcgac gtcgaggaga tgacccggac gtcgacctgt acgtcgttat cgccgcccac
     1501 gcgccggaag cgctgttcaa caagaacccg cagaatacgg ctctgtgtct cacgcggcat
     1561 gtcggccact tcgtcgaggt atagcgtgcc gccatgggcc cgctcgaaca cctgctctga
     1621 ggtttccatg gatacgctca gcgcc
//
LOCUS       DAOMEO010000800         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7574_length_1644_cov_3.073002, whole genome shotgun sequence.
ACCESSION   DAOMEO010000800 DAOMEO010000000
VERSION     DAOMEO010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7574_length_1644_cov_3.073002"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLH31_15525"
     CDS             <1..403
                     /locus_tag="PLH31_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90638.1"
                     /translation="LARANDVADRVLIGEAFHGEDFERFRGSKVLVFIDAEGFEDDIL
                     RPDLYPALTGFNLIVETHPLMRPGVTERLIERFSPTHDIVQIDQGIEKADMPDVLAKS
                     SHLEMVLATWEWRARPTPWLAMRPKSRASGA"
     gene            505..1605
                     /locus_tag="PLH31_15530"
     CDS             505..1605
                     /locus_tag="PLH31_15530"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR90639.1"
                     /translation="MRLDISTKVSLTIATAFLVMTAAVLAIVASGSLGFAKQQAAAQQ
                     EASMRVAWDVIGKQGGAFRREGDQLMVGDQVLNGDFSGVDRVKALVGGTATVFMGDTR
                     ITTNVTKPDGSRAVGTPLAKGKVHDAVLGRGESYRGEAKILDRPFFVAYDPIKDASGQ
                     VIGILYVGVPQADYFQPVYRQLGFLAAACLLLGAAGAGASVLMVRGQLKPLSALRDAM
                     SRVMAGDLTVSLGFAGRRDDIGLMADAVHAFRDDAREKLAIKAQSDARDGQAEAERRA
                     TQDRDARQAAAQTLVVSSLAQGLERLSNGDLTVRLETPFAADYEGLRADFNQTVARLE
                     DALGAVVDAVGGLNAGAGGIASEAVDLSRRTE"
BASE COUNT          269 a          565 c          539 g          271 t
ORIGIN      
        1 cctagccagg gccaatgacg tcgccgatcg ggtgctgatc ggcgaagcgt ttcacggcga
       61 ggacttcgaa cgttttcggg gctccaaggt gctggtgttc atcgacgccg aaggtttcga
      121 ggacgacatc ctgcggccgg acctttaccc cgccttgacc ggcttcaacc tgattgtcga
      181 gactcacccc ttgatgcgtc ccggcgtgac cgagcgcctg atcgagcggt tctcgccgac
      241 ccacgacatc gtccagatcg atcaggggat cgagaaggcc gacatgcctg acgtgctggc
      301 caaaagcagc cacctggaaa tggtgttggc gacctgggaa tggcgcgccc ggccgacgcc
      361 atggctggca atgcgaccca agagccgcgc ctccggggcg tgacattacg tcccagtttc
      421 gaaacccccg ccatttccgg gtgttaagcg gtacttcacg taaggtgaag catgatgggc
      481 gctcacgctc tccgaagagt aaccatgcgc ctggacattt ccaccaaggt cagcctgacc
      541 atcgctaccg cgtttctggt gatgaccgcc gccgttctgg ccatcgtcgc cagcggatcg
      601 ctcggctttg ccaagcagca ggcggcggcc cagcaggaag ccagcatgcg ggtggcctgg
      661 gacgtcatcg gcaagcaggg cggcgccttc cggcgcgagg gcgaccagct gatggtcggc
      721 gatcaggtcc tgaatggcga cttctccggc gtcgaccgcg tcaaggccct ggtcggcggc
      781 acagccaccg tcttcatggg cgacacccgg attaccacca atgtcaccaa gcccgacggc
      841 agtcgcgccg tcggcacgcc cctggccaag ggcaaggtcc atgacgccgt gctgggccgc
      901 ggcgagtcct atcgcggcga ggccaagatc ctggatcggc cgttcttcgt cgcctatgac
      961 cccatcaagg acgccagcgg tcaggtgatc ggcattctct atgtgggcgt gccccaggcc
     1021 gactacttcc agcctgtata tcgtcaactg ggcttcctgg cggcggcctg cctgctgctg
     1081 ggcgcggccg gagccggcgc ctcggtgctg atggtgcgcg gtcagctgaa gcccctgtca
     1141 gccctgcgcg acgccatgag ccgtgtcatg gccggtgatc tgaccgtcag cctgggcttt
     1201 gccggccggc gcgacgatat cggcctgatg gctgacgcgg tgcacgcctt ccgcgacgac
     1261 gcccgcgaga aactggccat caaggcccag tcggacgccc gcgacggcca ggccgaagcc
     1321 gagcgccgcg ccacccagga tcgtgacgcc cgccaggccg ccgcacagac gctggtggtc
     1381 tcaagtctcg cccagggtct ggagcgcctg tccaatggcg acctgaccgt tcgcctggag
     1441 actccgttcg ccgccgacta cgagggcctg cgcgccgatt tcaaccagac cgtggcccgc
     1501 cttgaggacg ccctgggcgc cgtggtcgac gcggtagggg gcctcaatgc cggcgccggc
     1561 ggcatcgcct cggaggccgt cgacctgtcg cgacgcaccg agtagcaggc cgccagcctg
     1621 gaggaaaccg ccgccgccct cgat
//
LOCUS       DAOMEO010000801         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7578_length_1644_cov_1.923851, whole genome shotgun sequence.
ACCESSION   DAOMEO010000801 DAOMEO010000000
VERSION     DAOMEO010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7578_length_1644_cov_1.923851"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLH31_15535"
     CDS             <1..581
                     /locus_tag="PLH31_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR90640.1"
                     /translation="HAHYDHAGGLAQMKAVTGAKLYASSLDKPALEKGRHFGDNENGL
                     TPFPAVKVDRTIRDGQKIKLGEATLTAHLTPGHTIGGTTWTMSVTERGRPLSVMFFNS
                     VSVAGNALVGNRTYPRIVADYRATFARLKAMPADVFLPVHPEQGGLIAKRQRVLNGDN
                     SAFIDPAELGRFIDASEAAFNKELARQQGAAK"
     gene            578..>1644
                     /locus_tag="PLH31_15540"
     CDS             578..>1644
                     /locus_tag="PLH31_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQR90641.1"
                     /translation="MTDLTTPDLSTRGGRIAALKAAAKERVLILDGSWGVMFQKKKLV
                     EADYRGQRFADYDGQMKGNNDVLCLTRPDLVAELHDAYFAAGTDISETNTFSGTTIAQ
                     ADYHLSEKDVWDINYEGARIGRSVADRWLKAHPEKPRFIAGSIGPLNVMLSMSSDVND
                     PGARKVTFDQVYEAYRQQVSALYEGGVDLFLIETITDTLNCKAAIKAILDLRDEGHEE
                     LPIWISGTITDRSGRTLSGQTAEAFWNSVKHCKPFAVGFNCALGADLMRPHIAEMARI
                     ADTLVAAYPNAGLPNAMGEYDEEPHQTGHALHEWATDGLVNILGGCCGSTPAHIAAMA
                     KAVAGLPARAVPVPPVVTMAST"
BASE COUNT          309 a          562 c          522 g          251 t
ORIGIN      
        1 gccacgccca ctacgaccac gccggaggcc tggcccagat gaaggcggtc acgggcgcca
       61 agctctacgc ctcgtcgctg gacaagcccg ccctggagaa gggccgtcac tttggcgaca
      121 acgagaacgg cctgacgcca ttcccggccg tgaaggtgga tcgcacgatc cgcgacggcc
      181 agaagatcaa gctgggcgag gccaccctga ccgcgcacct gacgcccggc cacaccatcg
      241 gcggcaccac ctggacgatg tcggtgaccg agcggggccg gcccctgagc gtgatgttct
      301 tcaactccgt ctcggtggcg ggcaacgccc tggtcggcaa caggacctat cccaggatcg
      361 tggcggacta ccgcgccacc ttcgccaggc tgaaggccat gccggctgat gtgttcctgc
      421 cggtccatcc cgagcagggc ggcctgatcg ccaagcgcca gcgggtgctg aatggcgaca
      481 acagcgcctt catcgatccg gccgaactgg gccgtttcat cgacgcctcg gaagccgcgt
      541 tcaacaagga actggcccgc cagcagggag cggccaaatg accgacctca ccacgcctga
      601 cctttcaacg aggggcggcc gcatcgccgc cctcaaggcc gccgccaagg agcgcgtact
      661 gatcctcgac ggctcctggg gcgtgatgtt ccagaagaag aagctggtcg aggccgacta
      721 tcgtggccag cgcttcgctg actacgacgg ccagatgaag ggcaataacg acgtcctgtg
      781 cctgacgcgg cccgatttgg tcgccgaact gcacgacgcc tatttcgcgg ccggcaccga
      841 catctccgag accaacacct tttccggcac caccatcgcc caggccgact atcacctgag
      901 cgaaaaggac gtctgggaca tcaactacga aggcgcgcgc atcggccgtt cggtggccga
      961 ccgctggctg aaggcgcatc cagaaaagcc gcgcttcatc gccggctcga tcggaccgct
     1021 gaacgtgatg ctgtcgatgt cgtcggatgt gaatgatccg ggcgcgcgca aggtgacctt
     1081 cgaccaggtc tacgaggcct atcgccagca ggtttcagcc ctgtacgagg gcggggtcga
     1141 tctctttttg atcgagacca tcaccgatac gctgaactgc aaggccgcga tcaaggcgat
     1201 cctggatctt cgcgatgagg gccacgaaga gctgccgatc tggatcagcg gcaccatcac
     1261 cgaccgttcg ggccgcaccc tgtcgggcca gacggccgag gcgttctgga acagcgtcaa
     1321 gcactgcaag ccgttcgccg tgggcttcaa ctgcgccctg ggcgctgatc ttatgcggcc
     1381 gcatatcgcc gagatggccc gcatcgccga caccctggtg gcggcctatc ccaatgccgg
     1441 cctgcccaac gccatgggcg aatatgacga ggagccgcac cagaccggcc acgccctgca
     1501 cgaatgggcg acggacggct tggtcaacat cctgggcggc tgctgcggat cgacacctgc
     1561 ccatatcgcg gcaatggcga aggccgtggc gggcctgcca gcccgcgccg tgccggtgcc
     1621 gccggtggtg acgatggcgt cgac
//
LOCUS       DAOMEO010000802         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7579_length_1644_cov_1.840780, whole genome shotgun sequence.
ACCESSION   DAOMEO010000802 DAOMEO010000000
VERSION     DAOMEO010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_1644_cov_1.840780"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLH31_15545"
     CDS             <1..196
                     /locus_tag="PLH31_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR90642.1"
                     /translation="GYIDTEMVAAVPENVLAQIIAGIPVGRLGKGEEIADMVSFLAGE
                     RAGFVTGATLTLNGGQYMAA"
     gene            368..1147
                     /locus_tag="PLH31_15550"
     CDS             368..1147
                     /locus_tag="PLH31_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4908 domain-containing protein"
                     /protein_id="HQR90643.1"
                     /translation="MKSSIVEMDGWRAKCVLVIVAMLAIASPVGARQTNQSLRDALLG
                     HRGFSTSRRPPPPPVARYVSETGSAFVLDRASPLPLLKFENSAEVWVLSPQPASRGDV
                     IYRNELGEAMLRATKLGGMILFTDERPNGAAAALVGKAASIQPPSVLSPIALYQRMGQ
                     ASVRASRAAQRQMPFESQDARPETSVLIAEAATVTAEAFERISRNGDRATLARITRVV
                     LAEGDKPGAVFRNGALIVTFAPGQGVAGRPSSKRIVKVVNR"
     gene            complement(1174..1614)
                     /locus_tag="PLH31_15555"
     CDS             complement(1174..1614)
                     /locus_tag="PLH31_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90644.1"
                     /translation="MSDEYHDARAAVRCATDYILRWVEIANETHGVDIVYSLVYTTLW
                     AGNVSHLRGRVYAEIDDIPPDAERRPITVRQVADSLGLPYETVRRRVVEMLKRGVAKK
                     VGREGFIVSGEALARPEMVAGLKRSHASLMRFLRELKALGIEAT"
BASE COUNT          248 a          566 c          548 g          282 t
ORIGIN      
        1 cggctatatc gacaccgaga tggtcgccgc cgtgccggaa aacgtcctgg cccagatcat
       61 tgccggcatc ccggtgggcc gcctgggcaa gggcgaagag atcgctgaca tggtctcgtt
      121 cctggccggc gagcgcgcgg gctttgtcac cggcgccacc ctgaccctga acggcgggca
      181 gtacatggcg gcttaagccg tccgctgtcg gcatgtttgc aacggcccgg cgcgcaagcg
      241 accgggccgt tgtcgcgacg gttgacgacc cgaggcgttt cgtcgtgaaa cctttgatcc
      301 gggccgcggg cgtctcgccg tggcgtcccg tccaagattc gccctagtcg gcgggcatga
      361 gaggttcatg aagagttcga ttgtcgagat ggacggctgg agggcgaaat gcgtcctcgt
      421 gatcgtcgcc atgctcgcca tcgcctcgcc ggtcggcgcc cggcagacga accagtcgct
      481 ccgggacgcc ctgcttggcc accggggctt ttcgacctcg cgtcgtccgc ccccgccgcc
      541 tgtggcccgc tatgtttccg agaccggcag cgcctttgtg ctggaccgcg cctcgccgct
      601 gccgctgctg aagttcgaga acagcgccga ggtctgggtg ctttcgcctc agcccgcctc
      661 gcgcggcgac gtgatctatc gcaatgagct gggcgaggcg atgctgcggg ccaccaagct
      721 tggcggcatg atcctgttta ccgacgagcg ccccaacggc gcggccgccg ccctggtggg
      781 caaggcggcg tcgatccagc cgccgtctgt gctgtcgccc atcgccctgt atcagcgcat
      841 gggccaggcc agcgtccgcg ccagccgcgc ggcccagcgc cagatgccgt tcgagtcgca
      901 ggacgcgagg cccgaaacct ccgtgctgat cgccgaggcc gccacggtca ccgccgaggc
      961 cttcgagcgc atctcccgca atggcgaccg ggcgacgctg gcccggatca cgcgggtcgt
     1021 actggccgaa ggcgacaagc cgggggctgt tttcagaaac ggcgccctga tcgtcacctt
     1081 cgcgcccggt cagggcgtgg cgggtcgccc gtcgtccaag cggatcgtca aggtcgtgaa
     1141 ccgctagcct attcgggcgc cagcctagtc ggctcaggtc gcctcgatac ccagggcttt
     1201 cagttccctc aggaaccgca tcaggctggc gtgcgagcgc ttgagtccgg ccaccatttc
     1261 gggccgggcc agggcctcgc cggacacgat gaagccctcg cggcccacct tcttggcgac
     1321 cccgcgcttg agcatttcga cgacgcggcg acggacggtt tcgtagggca ggcccagcga
     1381 gtctgcgacc tggcggacag tgatcggccg gcgctccgca tccggtggaa tgtcgtcgat
     1441 ctcggcatag acgcgccccc tcaggtggga gacgttgccc gcccagaggg tcgtgtagac
     1501 cagcgaatag acgatatcga cgccatgggt ctcgttggcg atctcgaccc aacgcaaaat
     1561 atagtccgtg gcgcagcgca ccgcggcgcg cgcgtcatgg tactcgtccg acatgttgac
     1621 cccacccatt tgtcagacct ttaa
//
LOCUS       DAOMEO010000803         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7581_length_1644_cov_1.686595, whole genome shotgun sequence.
ACCESSION   DAOMEO010000803 DAOMEO010000000
VERSION     DAOMEO010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_1644_cov_1.686595"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..676
                     /gene="pyrH"
                     /locus_tag="PLH31_15560"
     CDS             2..676
                     /gene="pyrH"
                     /locus_tag="PLH31_15560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQR90645.1"
                     /translation="MGDTPYGIDTNTVQSVAEDIAQIVKSGVELCIVIGGGNIFRGMA
                     GAAKGMERSSADYMGMLATVMNALAMQDALERVGVSTRVQSAIPMATVCEPYIRRRAQ
                     RHLEKGRVVIFAAGTGNPFFTTDTAAALRAAEMQCDALFKGTSVDGVYTADPKKDANA
                     QRYDRLTYMDVLAKDLRVMDASAVALMRDSNIPIVVFSIKGRGNLLNVLRGEGTFTVV
                     AQDAAA"
     gene            700..1266
                     /gene="frr"
                     /locus_tag="PLH31_15565"
     CDS             700..1266
                     /gene="frr"
                     /locus_tag="PLH31_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQR90646.1"
                     /translation="MAAAEKPVLSRYKERMEKSVQALKEDFAGLRTGRASSSLLDQIM
                     VNAYGSMVQLNTVASISVPEPRQINVNIWDKGVVSAVEKAIRNSDLGLNPVVEGNNLR
                     IPIPPLTEERRKDLSKIAGKYAEAQKVAVRNIRRDANDDLKKAEKASVISEDELKKME
                     TEVQKMTDDAIKKVDEALKIKEQEIMQV"
     gene            1312..>1644
                     /gene="uppS"
                     /locus_tag="PLH31_15570"
     CDS             1312..>1644
                     /gene="uppS"
                     /locus_tag="PLH31_15570"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQR90647.1"
                     /translation="MSATTGLQDVSVRAGGDASNRSLHVAIIMDGNGRWAKRRGMPRA
                     LGHRAGVNALKRTVEAAPELGVSVLTVFGFSTENWSRPAQEISELMGLLKAYVESDLV
                     RLAREGVRV"
BASE COUNT          333 a          527 c          523 g          261 t
ORIGIN      
        1 gatgggcgat acgccctacg gcatcgacac caacaccgtt cagtccgtgg ccgaggacat
       61 cgcccagatc gtcaagtccg gcgtcgagct ttgcatcgtg atcggcggcg gcaacatttt
      121 ccgcggcatg gccggcgccg ccaagggcat ggagcgctcc agcgccgact atatgggcat
      181 gctggccacg gtcatgaacg ccctggccat gcaggacgcg cttgagcgcg tcggcgtctc
      241 cacccgcgtg caatcggcca ttccgatggc gacggtttgc gagccctaca tccgccgccg
      301 ggcccagcgt cacctggaaa agggcagggt ggtgatcttc gccgccggca ccggcaaccc
      361 gttcttcacc accgacaccg ccgccgccct gcgcgccgcc gaaatgcaat gcgacgccct
      421 gttcaagggc accagcgtcg acggcgtcta cactgccgat cccaagaagg acgccaacgc
      481 ccagcgctac gaccgtctca cctacatgga cgtgctggcc aaggacctga gggtcatgga
      541 cgcctccgcc gtcgccctga tgcgcgacag caacattccg atcgtggtct tttccatcaa
      601 ggggcgcggc aatctgctga atgtcctgcg tggcgagggc acctttaccg tcgtcgccca
      661 agacgccgcc gcctaactca agcgaagaga gcccaagcca tggccgccgc cgaaaagccc
      721 gttctgtccc gctacaagga gcgcatggag aagtccgtgc aggctctgaa agaagatttc
      781 gccggtctgc gcaccggtcg cgcgtcgtcc agcctgctcg accagatcat ggtcaatgcc
      841 tatggctcga tggtgcagct caataccgtc gcctcgatca gcgtgccgga accgcgccag
      901 atcaacgtca acatctggga caagggcgtt gtctcggctg tggagaaggc gatccgcaac
      961 tcggatctgg gcctgaatcc ggtcgttgaa ggcaacaatc tgcggatccc gatcccgccg
     1021 ctgaccgaag agcgccgcaa ggacctctcc aagatcgctg gcaagtacgc cgaggcccag
     1081 aaggtcgccg tgcgcaacat ccgccgtgac gccaatgacg acctcaagaa ggccgagaag
     1141 gccagcgtca tcagcgagga tgaactgaag aagatggaaa ccgaggtcca gaagatgacc
     1201 gacgacgcca tcaagaaggt cgacgaggcc ttgaaaatca aagagcaaga gatcatgcag
     1261 gtctaagggc cgacctgagt aacggggacg gttagggaag gcttgaagcg aatgtcggcg
     1321 acgaccggcc tgcaggatgt ttcggtgcgc gctgggggcg atgcgtcgaa ccgctccctg
     1381 cacgtggcca tcatcatgga cgggaacggt cgctgggcta agcggcgcgg catgccgcgc
     1441 gcgctgggcc atcgcgccgg cgtcaatgcg ctcaagcgca ccgtagaggc tgcgccggag
     1501 ctgggcgtca gcgtactcac cgtgttcggc ttctcgaccg aaaactggag ccgccccgcg
     1561 caggagattt ccgagttgat gggtctgctg aaggcctatg tcgaatcgga cctggtgcgc
     1621 ctggcgcggg aaggcgtccg ggtt
//
LOCUS       DAOMEO010000804         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7584_length_1643_cov_2.350126, whole genome shotgun sequence.
ACCESSION   DAOMEO010000804 DAOMEO010000000
VERSION     DAOMEO010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7584_length_1643_cov_2.350126"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLH31_15575"
     CDS             complement(<1..692)
                     /locus_tag="PLH31_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HQR90648.1"
                     /translation="MRLPTLFGALGLLISLAVAPVARAEDGYDLWLRYRPVEAAARAP
                     YAAAGAAIAPTAQTPTLSLARAELERGLAGLLGAAPASAEAGGRDGVVLIGTPETSPA
                     IARLGLPLKAAGDEGYLIRTVRIDGKATTVIAANRDIGVLYGVFRYLSLIQTRQPVDN
                     LAITSAPKVQLRMLNHWDNLDRTVERGYAGQSIWDWWKLPDHKDARYADYARANASLG
                     INGTVLNNVNSKS"
     gene            921..>1643
                     /locus_tag="PLH31_15580"
     CDS             921..>1643
                     /locus_tag="PLH31_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HQR90649.1"
                     /translation="MPKIIAAKTIVTCPGRNFVTLKIITEDGTYGVGDATLNGRELAV
                     EAYLTEHVIPCLIGRDAHTIEDIWQYLYKGAYWRRGPVTMSAIAAVDMALWDIKGKLA
                     GLPLYQLLGGACRSGVMVYGHANGETIEETLDNAAAYLEQGYKAIRLQSGVPGMKGTY
                     GVSKDKFFYEPADSDLPKETLWSTEKYLRAAPELFKAARERLGSDVHLLHDVHHRLTP
                     IEAGRLGKDLEPYRLFWMEDCTP"
BASE COUNT          295 a          553 c          539 g          256 t
ORIGIN      
        1 gacttggaat taacgttgtt cagcaccgtg ccgttgatcc ccagcgaggc attggcgcgg
       61 gcatagtcgg cgtagcgggc gtccttgtgg tcgggcagtt tccaccagtc ccagatcgac
      121 tggcccgcat agccgcgctc gaccgtgcga tccagattgt cccagtggtt cagcatccgc
      181 agctgaacct tcggggcgga ggtgatggcc aagttatcca caggctggcg ggtctggatc
      241 agagagagat agcggaacac gccatacagc acgccgatgt cgcgattggc ggcgatcacg
      301 gtcgtggcct tgccgtcgat gcggaccgtg cggatcagat agccctcgtc gccggccgcc
      361 ttcagcggca gacccaggcg cgcgatggcg ggcgaggttt ccggcgtgcc gatcaggacg
      421 acgccgtcac gaccgccggc ctctgcggac gcgggtgctg cgcccagcag gccggcaagg
      481 ccgcgctcca gttcggcgcg ggcgagggac agggtcgggg tctgggctgt cggcgcgatc
      541 gccgcgccgg cggcggcata gggcgcgcgg gcggcggctt ccaccggcct gtagcgcaac
      601 caaaggtcat agccgtcctc ggcgcgggcg acaggcgcaa cggccaggct gatcagaagg
      661 cccagggccc cgaaaagggt cggcaatcgc atcgtctact ccctgatcgc cgcgttaccc
      721 ggcgctacct gcttatgctc cggccgtttc acgccagagg tcgagacact gacatctagt
      781 ggtctgacca ttgggttgac aacccatccc ccaccgctaa accgatatca gggaggaaaa
      841 ccacgccaac gcgccctggc gcgtgtcacg ggcatggtcg ccctcccggc tgcgtccacg
      901 aaagcgtctc ccgaagtccc atgcccaaga tcatcgccgc caagaccatc gtgacctgcc
      961 ctgggcgcaa cttcgtcacc ctgaagatca tcacggaaga cgggacctat ggcgttggcg
     1021 acgccacgct gaacggccgc gaactggcgg tcgaggccta tctgaccgag cacgtcatcc
     1081 cctgcctgat cggccgcgac gcacacacga tcgaggacat ctggcagtac ctgtacaagg
     1141 gcgcctactg gcgtcggggg ccggtgacaa tgtcggccat cgccgccgtc gacatggccc
     1201 tgtgggacat caagggcaag ctggccggcc tgccgctgta tcagctgctg ggcggcgcct
     1261 gccgctcggg cgtgatggtg tatggccacg ccaatggcga gaccatcgaa gagaccctcg
     1321 acaacgccgc cgcctatctc gagcagggct acaaggccat ccgcctgcag tcgggcgtgc
     1381 cgggcatgaa gggaacctac ggcgtctcca aggacaagtt cttctacgaa ccggccgata
     1441 gcgacctgcc caaggaaacg ctctggtcga ccgagaaata cctgcgcgcc gcgcccgaac
     1501 tgttcaaggc cgcccgcgag cggctgggct cggacgtcca cctgctgcac gacgtgcacc
     1561 accgcctgac gcccatcgag gccggccggc tgggcaagga tctggagccc tatcgcctgt
     1621 tctggatgga agactgcacc ccg
//
LOCUS       DAOMEO010000805         1642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7588_length_1642_cov_2.606805, whole genome shotgun sequence.
ACCESSION   DAOMEO010000805 DAOMEO010000000
VERSION     DAOMEO010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_1642_cov_2.606805"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..439)
                     /locus_tag="PLH31_15585"
     CDS             complement(32..439)
                     /locus_tag="PLH31_15585"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HQR90650.1"
                     /translation="MSDKPKADHAPGAISRARRLRRDMTASERHLWKALRALNLHIRR
                     QAPVGRYIADFIHHGACLVIEVDSAWHDSLKAQERDALRDAWFQSQGYRVLRFRDREV
                     FSDPYAIADRVAVEIQQSPPSQPFPHQGGRAQE"
     gene            579..1094
                     /locus_tag="PLH31_15590"
     CDS             579..1094
                     /locus_tag="PLH31_15590"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQR90651.1"
                     /translation="MPPPISLTLDRLTSPIGEILLVTDEAGRVRAIDFHDYASRMMRL
                     LRLHYGDTTLDEGVAPAAAREALTDYFDGQLDALPAISWATGGTAFQRTVWTALLDVP
                     AGTTSTYAALAARIGKPAATRAVGAANGANPIAILVPCHRLVGANGSLTGYAGGLERK
                     QWLLRHEGVRF"
     gene            complement(1091..1516)
                     /locus_tag="PLH31_15595"
     CDS             complement(1091..1516)
                     /locus_tag="PLH31_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HQR90652.1"
                     /translation="MSSYALSLLLAVIACVIGGALGGIALARPGQVLGLAGLAEGEAA
                     PTPLLAEGRAFGGLLVLSHAGAALYLGYQPGIGAAMAFALALAWFGAALGRAASLLID
                     QAGGRFNLGNLVFEVLIGVTLSLPFFSAGRLVLRGGMIA"
     gene            complement(1537..>1642)
                     /locus_tag="PLH31_15600"
     CDS             complement(1537..>1642)
                     /locus_tag="PLH31_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR90653.1"
                     /translation="RPHYHPDYYGAFVIDPDGFNIEAVFHGARATGPA"
BASE COUNT          296 a          565 c          523 g          258 t
ORIGIN      
        1 agcggccctg cacgtgccgg ggattcggta atcattcttg agcccttccc ccttgatggg
       61 ggaagggttg ggatgggggt gactgctgaa tctccaccgc gacacggtca gcgattgcat
      121 acggatcgct gaacacctcc cgatcccgaa atcgcagcac gcgatatcct tgggactgga
      181 accacgcatc acgcagggcg tcgcgttctt gcgccttgag cgaatcatgc caggcgctgt
      241 caacctcgat gaccagacaa gcgccgtgat ggatgaaatc ggcgatgtat cggccaaccg
      301 gagcctgcct tcggatatgg agattgagcg cccttagcgc cttccaaaga tgtctttcgc
      361 tggccgtcat gtcgcggcgc agtctgcggg cacggctgat cgcgccgggc gcgtggtccg
      421 cttttggctt gtcggacatg ccgcatcacc cccatccccg cccttccccc atcaaggggg
      481 aagggagaac aatatacttt tgattgtaat ttacatctca ctcccctgtt cacaagaacc
      541 ggaaagacgc agcggcggcg gcgggcgata aggctcgaat gccgccgccc atcagcctca
      601 ccctcgaccg cctcacctcg ccgatcggcg agatcctgct ggtcaccgac gaggccggcc
      661 gcgtgcgggc catcgacttc catgactatg cctcgcgcat gatgcggctg ctgcgtcttc
      721 actatggcga cacgacgctg gacgagggcg tcgcgccagc agccgcccgc gaggcgctga
      781 ccgactattt cgacggccag ctggacgctc tgccggccat ctcctgggct acgggcggaa
      841 ccgccttcca gcgcacggtc tggaccgccc tgttggatgt gcccgccggg accacctcga
      901 cctacgccgc ccttgccgcg cggatcggca agcctgccgc cacaagggcc gtgggggccg
      961 ccaacggggc caatccgatc gcgatcctcg tgccctgcca ccgcctggtc ggcgccaacg
     1021 ggtcgctcac cggctatgcc gggggacttg agcgcaaaca gtggctgcta aggcacgagg
     1081 gcgtgcggtt ctaggcaatc atcccgccgc gcagcaccaa ccgccccgca ctgaagaacg
     1141 gcagactgag ggtaacgccg atcagcacct cgaagacaag gttgcccaga ttgaacctgc
     1201 caccagcctg atcgatcagc agcgaggcgg cgcggcccag ggcggcgccg aaccaggcca
     1261 gggccagggc gaaggccatc gcggcgccga tgcctggctg gtagcccaga tagagggccg
     1321 ccccggcgtg ggacagcacc agaaggccgc cgaacgcccg cccttccgcc agcagcgggg
     1381 tcggcgccgc ctcgccctcg gccagccccg ccagaccaag cacctgaccc ggtctggcca
     1441 gggcgatgcc gcccagcgcg ccgccgatca cgcaggcgat cacggccagc agcagggaca
     1501 gggcatagct cgacatgcgc ttggcgctcc ttgaggtcag gccggtccgg tcgcccgggc
     1561 gccgtggaac acggcctcta tgttgaagcc gtcaggatcg atgacgaagg ctccatagta
     1621 gtctggatga taatgtgggc ga
//
LOCUS       DAOMEO010000806         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7601_length_1641_cov_2.414880, whole genome shotgun sequence.
ACCESSION   DAOMEO010000806 DAOMEO010000000
VERSION     DAOMEO010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7601_length_1641_cov_2.414880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLH31_15605"
     CDS             <1..459
                     /locus_tag="PLH31_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR90654.1"
                     /translation="KIQGLIQKGIDEGATLVSGGVGRPEGLDKGYYVKPTVFANVTNE
                     MTIAKEEIFGPVVAILGYETVDEAVKVGNDTEYGLAAYISGGDEEQVRKVASRLRAGQ
                     VNINGAGPDMMAPFGGYKMSGNGREWGDHAFGEFLETKAILGYAPKVAAE"
     gene            complement(573..1259)
                     /locus_tag="PLH31_15610"
     CDS             complement(573..1259)
                     /locus_tag="PLH31_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90655.1"
                     /translation="MFSPTHLRHDEIVERLVALRKNITAQDVGNAFADSLVTRRLDLR
                     SALGSFGAALNLPSHHFADSYGSLRCRTCWAFESSAAEDLNVLNFERFKWGGVRHSDP
                     LYALLDLTRFRTSAPESSASEGHALLRRLLEIAGNAPANTRPNDLVKLLKSLIPGNAS
                     QRRVAIQCLGYAGVLQSREHAGFFDTYPIHRAHPPEGKNDWSYPISWWRGHDGVNVAA
                     VRFYFPEVMA"
BASE COUNT          317 a          477 c          531 g          316 t
ORIGIN      
        1 aagatccagg gcctgatcca gaagggcatc gacgagggcg ccaccctggt cagcggcggc
       61 gtcggccgac ccgagggtct ggacaagggc tactacgtca agcccaccgt cttcgccaat
      121 gtcaccaacg agatgaccat cgccaaggaa gagatcttcg gcccggtcgt cgccatcctc
      181 ggctacgaga cggtcgacga ggcggtgaag gtcggcaacg acaccgaata cggcctggcg
      241 gcctatatct cgggcggcga cgaggagcag gtccgcaagg tcgcctcgcg gctgcgcgcc
      301 ggccaggtca acatcaacgg cgccggcccc gacatgatgg ccccgttcgg cggctacaag
      361 atgagcggca acgggcggga gtggggcgac cacgcgttcg gggagttcct cgagacgaag
      421 gcgatcctgg gttacgcgcc gaaggtggcg gcggagtagg tttgctcagt tagacgggtt
      481 tggaggggcg gggctgaaag gctccgcctc tttttctttg gcgagttctt tggcgaggcc
      541 ttcgggcacc tccggggggc atcagccttg gttcaggcca tgacctcggg aaagtagaac
      601 ctcaccgctg ccacgttcac cccgtcgtgg cctcgccacc aagatatcgg ataggaccaa
      661 tcgttctttc cttcgggtgg gtgagcgcga tggatcggat aggtgtcgaa aaagcccgcg
      721 tgttcacgcg attgcagtac gccagcatac cccaaacact ggatcgcgac ccgccgctgg
      781 ctggcgttac cagggatcaa tgactttagc agcttcacca agtcgttcgg tcgagtgttg
      841 gccggcgcgt tccccgctat ttcgagcaac cggcgcaaaa gggcgtgacc ttcgctcgcg
      901 gagctttcgg gtgccgacgt tctgaaccgc gtgaggtcca gcagcgcata taggggatcg
      961 ctatgcctga ccccgcccca cttgaatcgc tcaaagttca agacattcaa gtcttcggcg
     1021 gcggaacttt caaatgccca gcaagtcctg cagcgtagag atccatagga gtcggcgaaa
     1081 tggtgactgg gcaggttgag tgccgcgcca aaactgccaa gtgcagatcg tagatcaagt
     1141 cgacgtgtca cgaggctgtc ggcgaatgcg tttcctacat cctgggcagt gatgttcttt
     1201 cgcagcgcca caagtcgctc cacgatctca tcgtggcgca gatgcgtcgg cgagaacatg
     1261 tagccagcct cggtagcgag gcggagcgtt tcgggtgcgg ttgtacgatc gcgcttccat
     1321 cctgaggcag accaataggt gtcgaagaga atgcgctcgc ctgtcttatt gggcctccaa
     1381 gagcgctcgt cctgtggatt tgcactgaag ggcattaagg gctctcaagc tacgaatgac
     1441 gaggtgtgtg gggcgcggtt gttgtaacca ttacctcacg gtgcgctgag gggctcaaca
     1501 ccattcgcta gtgttgaaat ggacgcccac gcgtcctagc cagcccttca tctcttagag
     1561 gcagcagcgt gccggtctgg acaggtcagt caaggctcgc ctctggcgac cgcttcgcgg
     1621 cgacgcggag cgtcggcctt g
//
LOCUS       DAOMEO010000807         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7616_length_1639_cov_2.299874, whole genome shotgun sequence.
ACCESSION   DAOMEO010000807 DAOMEO010000000
VERSION     DAOMEO010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_1639_cov_2.299874"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="pnp"
                     /locus_tag="PLH31_15615"
     CDS             complement(<1..1508)
                     /gene="pnp"
                     /locus_tag="PLH31_15615"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQR90656.1"
                     /translation="MFEIMRKTIEWGGKSLVLETGRIARQADGAVLATMGETVVLATA
                     VFAKKAKPGQDFFPLTVNYIEKTYAAGKIPGGFFKREGRPSEKETLVSRLIDRPIRPL
                     FVKGFKNEVQVVCTVLQHDLENDPDIVAMVAASAALVISGAPFMGPIGGARVGYINGE
                     YVLNPTLDELKESKMDLVVAGTADAVMMVESEIQELSEEIVLGGVSFAHKSMQAVING
                     IIELAEHAAKEPFDFQPEDTDAIKAEVKKAVGKDLAEAYTIRAKGDRVAALSAAKAKA
                     VDTFAKSDANPAGIDPLKLISVFKELEADIVRRSILDTGIRIDGRTVDKVRPILGEVG
                     ILPRTHGSALFTRGETQAIVVATLGTGDDEQFIDALEGTYKESFLLHYNFPPFSVGET
                     GRMGSPGRREIGHGKLAWRALRPMLPAKEDFPYTIRLVSEITESNGSSSMASVCGASL
                     ALMDAGVPMKRATAGIAMGLILEGERFAVLSDILGDEDHLGDMDFKVAGTEEG"
BASE COUNT          259 a          496 c          564 g          320 t
ORIGIN      
        1 ccttcctcgg tgccggcaac cttgaagtcc atgtcgccaa gatgatcttc gtcgccgagg
       61 atgtcggaca gaaccgcgaa gcgctcacct tcgaggatga gacccatcgc gatgccggcg
      121 gtggcgcgct tcatcggcac acccgcgtcc atcagcgcca gcgaggcgcc gcagaccgaa
      181 gccatcgagg acgagccgtt cgactcggtg atctcggaga ccaggcggat ggtgtagggg
      241 aagtcttcct tggccggcag catcgggcgc agggcgcgcc aggccagctt gccgtggccg
      301 atttcgcggc ggccggggct gcccatccga ccggtctcac cgaccgagaa gggagggaag
      361 ttgtagtgca gcaggaagga ttccttgtag gtgccttcca gggcgtcgat gaactgctcg
      421 tcgtcgccgg tgcccagggt ggcgaccacg atcgcttggg tttcgccgcg cgtgaacagg
      481 gccgagccgt gggtgcgcgg caggatgccg acttcaccca ggatcggacg caccttgtcg
      541 acggtgcggc cgtcgatgcg gatgccggtg tcgaggatgg agcgacgaac gatgtcggct
      601 tccagttcct tgaacaccga gatcagcttc agcggatcga tgccggccgg gttcgcatcg
      661 ctcttggcga aggtgtcgac ggccttggcc ttggcggccg acagggccgc gacgcggtcg
      721 cccttggcgc ggatggtgta ggcttcagcc aggtccttgc cgacggcctt cttcacttcg
      781 gccttgatgg cgtcggtgtc ttccggctgg aagtcgaagg gctccttggc ggcgtgttcg
      841 gccagttcga tgatgccgtt gatcacggcc tgcatcgact tgtgggcgaa gctgacgccg
      901 cccaggacga tctcttccga gagttcctgg atttcggact cgaccatcat cacggcgtcg
      961 gcggtgccgg ccacgaccag gtccatcttg ctttccttca gctcgtcgag cgtcgggttc
     1021 agcacgtatt cgccgttgat gtagccgacg cgagcgccgc cgatcgggcc catgaagggg
     1081 gcgccggaga tcaccagggc ggccgaggcg gcgaccatgg cgacgatgtc gggatcgttc
     1141 tcgaggtcat gctgcagcac ggtgcagacg acctggactt cgttcttgaa gcccttgacg
     1201 aacagggggc ggatcggacg gtcgatcagg cgggaaacca gggtttcctt ttccgacgga
     1261 cgaccttcgc gcttgaagaa gccgcccggg atcttgcccg cagcgtaggt cttctcgatg
     1321 tagttgacgg tcagggggaa gaagtcctgg ccgggcttgg ccttcttggc gaacacggcg
     1381 gtggccagga cgacggtttc gcccatggtg gccagaacgg cgccgtcagc ctgacgggcg
     1441 atgcgaccgg tttcgaggac caggctcttg ccgccccact cgatcgtctt gcgcatgatt
     1501 tcaaacattt ttgcttcttt cggttccgaa ggacccattt cctccggcac ggacaggggc
     1561 gggcggccga atggcggtcc cgagtcttcc tcatccagtt cgacaggcgt ccggggatcg
     1621 tgatctccgg cttacgtgt
//
LOCUS       DAOMEO010000808         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7617_length_1639_cov_2.266414, whole genome shotgun sequence.
ACCESSION   DAOMEO010000808 DAOMEO010000000
VERSION     DAOMEO010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_1639_cov_2.266414"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLH31_15620"
     CDS             complement(<1..342)
                     /locus_tag="PLH31_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HQR90657.1"
                     /translation="MTQTTSAGREALAGKALGVSARLWIFTALAGQLAFALYVASFYG
                     GAAVQGRFEAWNDVLVGGYVRGAMIGNIVLAAHLLLAVVITLGGPFQLWPWIRRRAPW
                     LHRWNGRFYMLT"
     gene            412..1476
                     /locus_tag="PLH31_15625"
     CDS             412..1476
                     /locus_tag="PLH31_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR90658.1"
                     /translation="MTAALGKHDPGIQPGGKAIAGTVSAGFADHLMRFAIAQGADPGA
                     LADRSGIRPEDLENRDHRIPARAYVALMGAAKALCGAPDLALRLGAASDFREISVVGL
                     ICYAAPTMGEALKELNRYGRLAMEVDLPAGAQRFAMVQRDGQLWLTDTRTDPNDFPEL
                     TESTWSRFICETARHFPDAPFARAVHVTHARPDHGAAYEALWKVPVSFGAAWNAIQID
                     PSWLHIPLHNPSGYAFGVLSDHADRLLAQLEASRTVRGRVESALLPILHKGAPGMEET
                     AARLGVSRPTLYRQLRQEGVTYEAVIDALRHTMALHYLNGRKVSVNETAYLVGFSDPS
                     AFSRAFKRWTGSSPRRLKAG"
     gene            complement(1478..>1639)
                     /locus_tag="PLH31_15630"
     CDS             complement(1478..>1639)
                     /locus_tag="PLH31_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR90659.1"
                     /translation="PHTCIGKRVAQIQLEEAYRQILARFPDLKATGEMEIAPNNFVHA
                     ISKLMVKRG"
BASE COUNT          268 a          584 c          528 g          259 t
ORIGIN      
        1 ggtcagcata tagaaccggc cgttccagcg gtgcagccag ggcgcccgcc ggcggatcca
       61 cggccaaagc tggaacggtc cgcccagggt gatgaccacc gccagcaaca gatgcgccgc
      121 gagcacgatg ttgccgatca ttgcgccgcg cacatagccg ccgaccagga cgtcgttcca
      181 ggcctcgaac cgtccctgaa ccgccgcgcc gccgtagaac gatgcgacgt acagagcaaa
      241 ggccagttgc cccgccaggg cggtgaagat ccaaagccgc gccgagacgc ccagggcctt
      301 gccagcaagc gcctcccggc cggctgaagt cgtttgcgtc atgcgttttc cctgacctga
      361 cacggctgcg cgtgttgcca acaggctagg tcagcggcca agacttgggc catgacggca
      421 gcgctcggaa aacatgaccc tgggattcag ccgggcggca aggcgatcgc cggcacggtc
      481 tccgccgggt ttgccgatca cctgatgcgc ttcgcgatcg cccagggcgc ggatcccggg
      541 gcgctggcgg accggtccgg cattcgacct gaagacctcg agaaccggga ccatcgcatc
      601 cccgctcgcg cctatgtcgc cctgatgggc gcggccaagg ccctgtgcgg cgcgcccgat
      661 ctggccctgc gactgggggc ggccagcgac tttcgcgaaa tctcggtcgt cgggctgatc
      721 tgctacgcgg ccccgaccat gggcgaagcg ctgaaggagc tgaaccgcta tgggcggctg
      781 gccatggagg tcgaccttcc cgccggggcg cagcgcttcg cgatggtcca gcgcgacggc
      841 cagctgtggc tgaccgacac gcggaccgac cccaacgact ttcccgaact gaccgagtcg
      901 acctggtcgc gcttcatctg cgagaccgcc cgccattttc ccgacgcccc gttcgccagg
      961 gccgtccacg tcacccacgc ccgaccagac cacggcgcgg cgtacgaggc cctgtggaag
     1021 gttccggtgt cctttggcgc agcctggaac gccatccaga tcgatccgtc ctggctgcac
     1081 atcccgctgc acaatcccag cggttacgcc tttggcgtcc tcagcgacca tgccgaccgg
     1141 ctgctggccc agctggaagc ctcgcgcacc gtgcggggcc gggtcgagtc ggccctactg
     1201 ccgatcctgc acaagggcgc gcccggcatg gaagaaacgg ccgcccgcct tggggtgagc
     1261 cggcccacgc tctaccgcca gcttcgccag gaaggcgtga cctatgaggc cgtcatcgac
     1321 gcccttcgcc acaccatggc cctgcactat ctgaatggcc gcaaggtgtc ggtgaacgag
     1381 acggcctatc tggtcggctt ctccgacccc agcgccttct cgcgcgcctt caagcgctgg
     1441 accggatcga gccccaggcg cctgaaggcg gggtgattta cccccgcttg accatcagct
     1501 tgctgatcgc gtgcacgaag ttgttgggcg cgatctccat ttcgccggtc gccttcaggt
     1561 cggggaaccg cgccaggatc tggcgatagg cttcctccag ctggatctgg gccacgcgct
     1621 tgccgatgca ggtgtgagg
//
LOCUS       DAOMEO010000809         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7630_length_1636_cov_2.872865, whole genome shotgun sequence.
ACCESSION   DAOMEO010000809 DAOMEO010000000
VERSION     DAOMEO010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7630_length_1636_cov_2.872865"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="cysE"
                     /locus_tag="PLH31_15635"
     CDS             <1..742
                     /gene="cysE"
                     /locus_tag="PLH31_15635"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQR90660.1"
                     /translation="EPALASLLNAVILSHDNIADALTFQLARKLGDQEMRAMTAREFA
                     ADAFNSDPALIAAAEADLMAVFERDPACKGYVQPFLFFKGFLALQTHRVSHWLWNEGR
                     ETLAFYLQSRASEVFQVDINPAARIGKGVFIDHGTGIVIGETAVVGDDVSMLHGVTLG
                     GTGADRGDRHPKIGNGVLLGAGAKVLGNITVGDYAKIASGSVVLKPVPPHCTAAGVPA
                     RIVNCPTCDEPAKSMDQTLSEAVYDYVI"
     gene            782..1510
                     /locus_tag="PLH31_15640"
     CDS             782..1510
                     /locus_tag="PLH31_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HQR90661.1"
                     /translation="MLAATAVAPAMAGSSRDYGYTVVNTYPHDSRAFTQGLFWRDGFL
                     YESTGLHGRSSVRKVTLETGVPEQERLIDSHYFGEGVVDWKDQVGFIFDLATFEKVGE
                     FSYPGEGWALTRDETRLIMSDGTAFLRFLDPDTLKETGRIQVTDKGKPVDNLNELEWV
                     KGEVLANIWQSDRVARIDPTTGQVTGWVDLSGLLGSADQAGGRVDVLNGIAYDAATDR
                     LFVTGKLWPRLFEIKLTPKAGLAH"
BASE COUNT          281 a          543 c          530 g          282 t
ORIGIN      
        1 cgagcccgcc ctggcctcgc tgctcaatgc ggtgatcctc agccacgaca acatcgccga
       61 cgccctgacc ttccagctgg cgcgcaagct gggcgaccag gaaatgcggg ccatgacggc
      121 gcgcgagttc gccgccgacg ccttcaattc cgatccggcc ctgatcgccg ccgccgaggc
      181 cgatctgatg gccgtgttcg agcgtgatcc tgcctgcaag ggctatgtcc agccgttcct
      241 gttcttcaag ggcttcctgg ccctgcagac ccaccgcgtg tcgcactggc tttggaacga
      301 ggggcgcgag accctggcct tctacctgca gagccgggcc agcgaggtct tccaggtcga
      361 tatcaacccc gccgcccgca tcggcaaggg cgtcttcatc gatcacggca ccggcatcgt
      421 catcggcgag acggcggtgg tcggcgacga cgtctcgatg ctgcacggcg tgaccctggg
      481 tggcacgggc gcggatcgcg gcgaccgcca tcccaagatc ggcaacggcg tgctgctggg
      541 cgcgggcgcc aaggtgctgg gcaatatcac cgtcggtgac tacgccaaga tcgcctcggg
      601 gtcggtggtg ctcaagcccg tgccgccgca ttgcaccgcc gccggggttc cggcccgcat
      661 cgtcaactgt ccgacctgtg acgagccggc caagtcgatg gaccagaccc tgtccgaggc
      721 cgtgtacgac tacgtgatct aggtgctcgg cagaatctgg aaaggcctgt gcctcgcggc
      781 cctgctggcc gcgacggccg tcgcgccggc catggccgga tcgtcgcgcg attatggcta
      841 caccgtcgtc aacacctatc cgcacgattc gcgggccttc actcaaggcc tcttctggcg
      901 cgacggcttt ctgtatgaga gcacgggcct gcatggtcgc tcgtccgtcc gcaaggtgac
      961 gctggaaacc ggcgtgcccg agcaggagcg cctgatcgac agccactatt tcggcgaggg
     1021 cgtcgtcgac tggaaggacc aggtgggctt catcttcgat ctggccacct tcgaaaaggt
     1081 cggtgagttc tcctatcccg gagagggctg ggccctgacc cgcgatgaga cgcggctcat
     1141 catgagcgac ggcacggcgt ttctgcgctt cctcgatccc gacaccctga aagagaccgg
     1201 acgcatacag gtcaccgaca agggcaagcc tgtggataac ctcaacgaac tggaatgggt
     1261 gaagggcgag gtcctggcca atatctggca gagcgaccgc gtcgcacgca tcgatccgac
     1321 cacgggccag gtgacgggct gggtcgatct gtcgggcttg cttggctctg ccgatcaggc
     1381 cggggggcgg gtcgatgtgc tgaacggcat tgcctatgac gcggcgaccg atcgtctctt
     1441 cgtcacaggc aagctttggc ccaggctgtt cgagatcaag ctgacgccga aggccggttt
     1501 ggcgcattag cgctgaagcg acttgcgact ccgccgcagg gcgctagttt cgccccggca
     1561 cgcgccacgg cgccaaacac aacgacagga gctccccgtg gacgctgaat ccatcgccaa
     1621 gatcgaaaag cacctg
//
LOCUS       DAOMEO010000810         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7664_length_1632_cov_2.884591, whole genome shotgun sequence.
ACCESSION   DAOMEO010000810 DAOMEO010000000
VERSION     DAOMEO010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_1632_cov_2.884591"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLH31_15645"
     CDS             complement(<1..711)
                     /locus_tag="PLH31_15645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR90662.1"
                     /translation="MEEPGDAQFDRIDTGDVRVPDAGVLRPRHAATLIIVRTDAAKPR
                     LLMGRRAGGHAFMPDKWVFPGGRVDRGDYRAPSATELSPEVAARLTHEPRHPSPATLA
                     RALGLAAIRETFEETGLLLAKSAPSRPAAGAWRPFLAQGALPDLAPLAFVARAITPPY
                     RTRRFDARFFMAPAEALLSLERQPDCGELDEIAWVDFDEAMALDLPNITRFVVAEIGQ
                     RLKDAGRPAPFMRFLNGGR"
     gene            823..>1632
                     /locus_tag="PLH31_15650"
     CDS             823..>1632
                     /locus_tag="PLH31_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR90663.1"
                     /translation="MSGRVSTLAIMAGLAFGAPALAAPSAAQIEAAAKAVQPRVVAWR
                     RDIHQHPELGNQEVRTAALVARELKALGFEVREGVGRTGVVGVLKGGQPGKVVALRAD
                     MDGLPVEEKTGLPFASKVTATWEGKTVPVMHACGHDTHVAMLLGAATVLAGMKAHIKG
                     TVVLIFQPAEEGPQAGEEGGAKLMIRDGVLDQPKVEAIFGLHIGPADAHRLNYRAGGF
                     YAGSERMTITVKGKQTHGARPWAGVDMASIAADIVQAMNQIAARQIDVGASP"
BASE COUNT          253 a          550 c          604 g          225 t
ORIGIN      
        1 ccggccgcca ttcaggaagc gcatgaaggg cgcgggacgt ccagcatcct tcagcctctg
       61 gccgatttcg gcgaccacga agcgggtgat gttgggcagg tccagggcca tggcctcgtc
      121 gaaatccacc caggcgatct cgtccagctc gccgcagtcg ggctggcgct ccaggctgag
      181 cagggcctcg gcgggcgcca tgaagaaccg ggcgtcaaag cggcgggtgc gatagggcgg
      241 ggtgatggcc cgggccacga aggccagcgg cgccaggtcg ggcagggcgc cctgggccag
      301 gaagggccgc caggccccgg cggcgggccg tgagggcgca gatttggcca gcagcaggcc
      361 cgtctcctcg aaggtctcgc ggatggccgc caggcccagg gcgcgggcca gggtggcggg
      421 gctgggatgg cgcggctcgt gggtcaggcg agcggcgacc tcgggcgaaa gctcggtggc
      481 tgacggcgcg cgatagtcgc cgcgatccac ccggccgccg gggaacaccc acttgtcagg
      541 cataaaggcg tggccgccag cccggcggcc catcagcagg cgcggcttgg cggcatcggt
      601 gcggacgatg atcagggtgg cggcgtggcg cggtcgcagc acgccggcgt cgggcacacg
      661 aacgtcgccg gtgtctatgc ggtcgaattg ggcgtcgcca ggctcttcca tggggctagc
      721 ttatccccat cgcgccattc gcaaagccgc cccttacgtc agcttgaccg ccgggacatc
      781 agcgccgcac tctccgacag cttttcaagg ggaatgacgc gcatgtctgg acgggtttcg
      841 acgctggcga tcatggccgg cctggccttt ggcgcgccgg ccctggcggc cccttccgcc
      901 gcccagatcg aggccgccgc caaggcggtg cagcccaggg tcgtcgcctg gcgccgcgat
      961 atccaccagc accccgaact gggcaatcag gaggttcgca ccgccgccct ggtcgcccgg
     1021 gagctgaagg ctctgggttt cgaggtgcgc gaaggcgtgg gcaggaccgg cgtcgtgggc
     1081 gttctgaagg gcggccagcc cggcaaggtg gtggccctgc gcgccgacat ggacggtctt
     1141 cccgtcgagg aaaagaccgg cctgccattt gcgtccaagg tgacggccac ctgggagggc
     1201 aagaccgtcc cggtcatgca cgcctgcggc catgacactc acgtcgccat gttgctgggc
     1261 gcggccaccg tgctggccgg catgaaggcc cacatcaagg gcacggtcgt cctgatcttc
     1321 cagccggctg aggaaggccc tcaggccggc gaggaaggcg gcgccaagct gatgatccgc
     1381 gacggggtgc tggaccagcc caaggtggag gcgatcttcg gcctgcacat tggtccggcc
     1441 gacgcgcaca ggctgaacta tcgcgccggg ggcttctacg ccggctccga gcgcatgacg
     1501 atcacggtca agggcaagca gacccacggc gcgcggccct gggcgggcgt cgacatggcc
     1561 agcatcgccg ccgacatcgt ccaggccatg aaccagatcg ccgcccgcca gatcgatgtc
     1621 ggcgcctcgc cc
//
LOCUS       DAOMEO010000811         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7665_length_1632_cov_2.644261, whole genome shotgun sequence.
ACCESSION   DAOMEO010000811 DAOMEO010000000
VERSION     DAOMEO010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7665_length_1632_cov_2.644261"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLH31_15655"
     CDS             complement(<1..139)
                     /locus_tag="PLH31_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQR90664.1"
                     /translation="MAIRRILTVDNAADLAVLKKISTPVEAVTDDLRALMDDMLETMY
                     DA"
     gene            complement(148..1458)
                     /locus_tag="PLH31_15660"
     CDS             complement(148..1458)
                     /locus_tag="PLH31_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQR90665.1"
                     /translation="MNFSDPFLILAAVFAAIAVAAILWALASTKRASAADARAWDLNA
                     RLVQADERARLLEDQAATQAELIRAQAAQQATMTANTVAEALIKRADENFRSREMLSQ
                     QRLEAQLKPVAETLAKFEAQVTAVEKSRAEETGGLKAQIAALMEASTATQFEARKLSA
                     ALRRGAGVQGRWGEQTLRNVLEAAGLNKRFDFEEQFSVDTEEGRRRPDVKVRMPGGAV
                     FVIDAKCSLNAFLEAQEMAEELPREAALLRHAQSVRAHMTSLSAKTYWDQFAAEGSPD
                     FVAMFVPGDSFLAAALERLPDLMTEAMDKRVLLVTPTTLFALCKAVAYGWRVEDQAKN
                     AANIVAVGRELYKRVAVMGAHAGAVGKALESAVGKYNQFVGSLESQVLTQARRFEDLS
                     VDHEGKEIAELAPVEGGVRALTKLAPQAAMGQSPTLTLGNETLT"
BASE COUNT          236 a          538 c          569 g          289 t
ORIGIN      
        1 gggcgtcgta catggtctcc agcatgtcat ccatcagggc gcgcagatcg tcggtcaccg
       61 cctcgacggg ggtggagatc ttcttcaaca ccgccagatc ggcggcgtta tcgacggtga
      121 ggatgcgacg gatagccatg ataggtgtca ggtaagggtc tcatttccca gcgtcaaggt
      181 gggggattgt cccatcgctg cctgaggggc cagcttggtc aacgcgcgaa ccccgccttc
      241 gaccggcgcc agttcggcga tttccttgcc ctcgtggtcg accgaaaggt cctcaaagcg
      301 acgggcctgg gtcaggacct ggctctccag cgagcccacg aactggttgt atttgcccac
      361 ggcgctttcc agcgccttgc ccaccgcgcc ggcgtgggcg cccatcaccg ccacgcgctt
      421 gtagagctcg cggccgaccg cgacgatatt ggcggcgttc ttggcctggt cctcgacccg
      481 ccagccatag gccaccgcct tgcacagcgc gaacagcgtc gtgggcgtga ccagcagcac
      541 ccgcttgtcc atggcctcgg tcatcaggtc gggcaggcgc tccagcgcgg cggccaggaa
      601 gctgtcgccg ggcacgaaca tggccacgaa gtcgggcgag ccttctgcgg cgaactggtc
      661 ccaataggtc ttggccgaca ggctggtcat gtgggcgcgc acgctctggg cgtggcgcag
      721 cagggcggcc tcgcgcggca gctcctcggc catttcctgg gcttccagga aggcgttcag
      781 cgagcacttg gcgtcgatca cgaacaccgc cccgcccggc atccgcacct tcacgtcagg
      841 ccggcggcgg ccctcctcgg tgtcgacgct gaactgctcc tcgaagtcga agcgcttgtt
      901 gaggccggcg gcctcaagga cgttccgcaa ggtctgctcg ccccagcggc cctgcacgcc
      961 ggcgccgcga cgcagggcgg ccgacagctt gcgggcttcg aactgggtcg cggtcgaggc
     1021 ctccatcagg gcggcgatct gggctttcag gccgccggtc tcctcggcgc gcgatttctc
     1081 gacggcggtg acctgggcct cgaacttggc cagggtctcg gcgacgggct tgagctgggc
     1141 ttccagccgc tgctgcgaca gcatttcccg gctgcggaaa ttctcgtcgg cgcgcttgat
     1201 cagggcctcg gccacggtgt tggcggtcat ggtcgcctgc tgcgcggcct gggcgcggat
     1261 cagctcggcc tgggtggcgg cctggtcttc cagcagccgg gcccgctcgt cggcctggac
     1321 cagacgggcg ttcagatccc aggccctggc gtcggcggcg ctcgcgcgct tggtcgaggc
     1381 gagcgcccac aggatggcgg ccacggcgat ggcggcgaac acggcggcga ggatcaggaa
     1441 cggatcggag aagttcatgc tccgttccta gccggagtcg gagtgtgttg gaaggcggct
     1501 tacggcgctg gtcgagaagg cgatgaccgc ttttgacgca aacctatctt gtcatcccgg
     1561 ccgcagcgaa gcggagcgcc ggcgactgtg ttggatcaag cgggtttttg ccaaggctgt
     1621 cccgacttga gc
//
LOCUS       DAOMEO010000812         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7676_length_1631_cov_2.381345, whole genome shotgun sequence.
ACCESSION   DAOMEO010000812 DAOMEO010000000
VERSION     DAOMEO010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7676_length_1631_cov_2.381345"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>440
                     /locus_tag="PLH31_15665"
     CDS             <1..>440
                     /locus_tag="PLH31_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR90666.1"
                     /translation="FAAVGAVVGGGRYLVRPLFRFIAKARLREIFTASALLIVVGVAS
                     LMQMVGLSPALGAFLAGVVLAESEFRRELETDIEPFRGLLLGLFFITVGAGVDLPLIA
                     SQPLTLIGLVLGLMVLKFLVMLGLARLFGAPLRGAAAVATALAQ"
     assembly_gap    441..450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <451..1390
                     /locus_tag="PLH31_15670"
     CDS             <451..1390
                     /locus_tag="PLH31_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQR90667.1"
                     /translation="LPAALAGRLSGVTAFSPVHPVDEVRAVGMLDECAATVGAGVIGA
                     TLAALLTAAVAVSMALTPLAMILYERVAAALDAAIPDVTPETGDFDDGEPEIIIAGFG
                     RFGQITGRLLTANGFRSTVLDSDIEQIDLLRRFGRRVHYGDATRMDLLRAAGIERARM
                     LIVALDDREKTVELVETARKAFPDLVILARAWDRRHAYDLLANGADAVERETFESALA
                     LGQTALQKLGFRAHRAHRAASFFRRHDRRTFEDLRPVWGQEEAYILASRDAAQTMDKL
                     LAADLSRMRPGDAGGAWDTASLEQELRERAEREGAD"
BASE COUNT          207 a          559 c          577 g          278 t
ORIGIN      
        1 gttcgccgcc gtgggcgcgg tggtcggggg cgggcgctat ctggtgcggc cgctgtttcg
       61 cttcatcgcc aaggcacggc tgcgcgagat cttcaccgct tcggccctgc tgatcgtcgt
      121 cggcgtggcc agcctgatgc agatggtcgg cctgtcgccc gcgctcggcg ccttcctggc
      181 cggggtggtt ctggccgaga gcgagttccg ccgcgaactg gaaaccgaca tcgagccctt
      241 ccgggggctg ctgctgggtc tgttcttcat cacggtcggg gcgggcgtcg acctgccgct
      301 gatcgccagc cagcccctga ccctgatcgg cctggtgctg ggcctgatgg tgctgaagtt
      361 cctggtcatg ctgggcctgg cccgcctgtt tggcgcgccc ctgcgcgggg ccgccgctgt
      421 ggccacggcc ctggcccagg nnnnnnnnnn gcttccggcg gcactggccg gccggctcag
      481 cggcgtcacc gccttctccc cggtacaccc ggttgacgag gtccgggccg tgggcatgct
      541 cgacgagtgc gcggccaccg tcggcgccgg ggtgatcggc gcaaccctgg cggcgctgct
      601 gaccgccgcc gtcgccgtct ccatggccct gacccccctg gcgatgatcc tctatgagcg
      661 ggtcgccgcc gcccttgacg ccgcgatccc cgatgtcacg cccgagaccg gtgatttcga
      721 cgatggcgag cccgagatca tcatcgccgg cttcggccgg tttggtcaga tcaccggccg
      781 cctgctgacc gccaacggct ttcgatcgac ggtgctggac agcgatatcg agcagatcga
      841 cctgctgcgc cggttcgggc ggcgggtgca ctatggcgac gccacccgga tggacctgtt
      901 gcgagccgcc ggcatcgagc gggcgcggat gctgatcgtg gcgctggatg atcgggaaaa
      961 gacggtcgag ctggtcgaaa cggcccgcaa ggcctttccg gatctggtga tcctggcccg
     1021 ggcctgggat cgccgccatg cctatgacct gctggccaat ggcgccgacg ccgtcgagcg
     1081 cgagaccttc gagtcggccc tggccctggg ccagaccgcc ttgcagaaac tggggttcag
     1141 ggcccaccgc gcgcaccgcg ccgcgtcctt cttccgccgg cacgaccggc gcaccttcga
     1201 ggacctgcgt ccggtgtggg gtcaggaaga ggcctatatc ctggcctcgc gcgacgccgc
     1261 ccagaccatg gacaaactac tggccgccga cctgtcgcgc atgcggccgg gggacgccgg
     1321 cggggcgtgg gacacggcga gcctggagca ggagctgcgc gagcgggccg agcgtgaagg
     1381 ggcggattga tccttctccc cttgcgggag aaggtgtcag ccgcaggctg acggatgagg
     1441 ggtcgcgctg acgcgggccg cctcatccgt cgcttcgtta tttgccggaa aggactatcg
     1501 acccctcatc cgacccgctg cgcgggccac cttctcccgc agggggagaa ggcagttcga
     1561 caattactgc gtgatcggac gcagaacgat ctcgacccgg cggttctggc tgcggccttc
     1621 ggccgtgcca t
//
LOCUS       DAOMEO010000813         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7677_length_1631_cov_1.828680, whole genome shotgun sequence.
ACCESSION   DAOMEO010000813 DAOMEO010000000
VERSION     DAOMEO010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_1631_cov_1.828680"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLH31_15675"
     CDS             complement(<1..1028)
                     /locus_tag="PLH31_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HQR90668.1"
                     /translation="MGGFRATLLVSAALAVVGGGAAAEDRVLTSPDKTLSITLSDTGG
                     QARYAVSYKGRTLIAPSALGLLLDKGDALSTGLSITGSSTASVDQTYELVVGKTRLVR
                     DHYNELTIDLAEAGPDGRKLRVIARAYDDGVAFRYVLPDQPRLAGVVIRNEETRFEIA
                     GDNRCWALNLGRFGTSHEGEYDPVLASSFREHNLLELPVVCQTPHGATFALAEADLKN
                     YAGLYLTGRGDSGIGLRAKLSPRLEDGAVAVHTRIGTDTMSSWRVVMVGDQPGDLISS
                     NLMTSLNPPTALTDTAWIKPGKAAWDWWNGSIVAGADKPAMDTATMKRFIDFAAEAED
                     GLADPGAD"
     gene            1278..1439
                     /locus_tag="PLH31_15680"
     CDS             1278..1439
                     /locus_tag="PLH31_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90669.1"
                     /translation="MCQRQYEEWASLWEALFGEPPPIAAEISLTARILVACLPSAEPY
                     EPGSKPPAS"
     gene            complement(1420..>1631)
                     /locus_tag="PLH31_15685"
     CDS             complement(1420..>1631)
                     /locus_tag="PLH31_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR90670.1"
                     /translation="PLLKRLEAGGLIARARDPQDERRVIITLTDRGQALRDQAAKIPE
                     KILCQLDLPLEDLGTLRDRLKTLAV"
BASE COUNT          244 a          532 c          572 g          283 t
ORIGIN      
        1 tcgtccgcac cgggatcggc aaggccgtcc tctgcctcgg cggcgaagtc gatgaaccgc
       61 ttcatcgtcg cggtatccat ggcgggcttg tcggctcccg cgacgatgga gccgttccac
      121 cagtcccagg cggccttgcc gggcttgatc cacgcggtgt cggtcagggc ggtcggcggg
      181 ttgaggctgg tcatcaggtt ggacgagatc aggtcgcccg gctggtcgcc gaccatcacc
      241 acgcgccagg atgacatcgt gtcggttccg atccgcgtgt gcaccgccac cgcgccgtcc
      301 tccagacggg gcgatagctt ggccctcagg ccgatcccgc tgtcgccgcg accggtcagg
      361 taaaggccgg catagttctt caggtcggcc tcggcgaggg cgaaggtcgc gccgtgcggt
      421 gtctggcaga ccacgggcag ctcaagcagg ttgtgctcgc gaaagctgga ggccaggacc
      481 ggatcgtatt cgccctcgtg cgaggttccg aaccgcccga gattcagcgc ccagcagcgg
      541 ttgtcgccgg cgatctcgaa acgggtttcc tcgttgcgga tcaccacgcc cgccaggcgc
      601 ggttggtccg gtaacacgta gcgaaaggcc accccgtcgt cataggcgcg ggcgatcacg
      661 cgaagcttgc gcccgtccgg gccggcctcg gcgagatcga tggtcagttc gttgtagtga
      721 tcgcggacca ggcgggtctt gccgaccacc agttcatagg tctggtcgac gctggccgtg
      781 ctggacccgg tgatcgaaag cccggtcgac agggcgtcgc ccttgtcgag caacaggccc
      841 agggcggatg gggcgatcaa cgtcctgccc ttgtagctca ccgcgtagcg ggcctggccg
      901 ccggtgtccg acagggtgat cgacagggtc ttgtccggtg acgtcagcac gcgatcctcg
      961 gccgcagcgc cgccgccgac caccgccagc gccgcgctga ccagtaaggt tgccctgaag
     1021 ccgcccatcg aatcctcccg tgaccgtgtt ttcattgtat ttgtcagaca attaaacggt
     1081 cctggcggcg gcgtcgagcc ttgggatacc ggtaccatca acattgcggc gcacctgtcg
     1141 caggtggacg ggccacctga cccaaccgtc gggctctggc gggtgcgggg cagggggggc
     1201 gagaaagcct ggttagcgcg ccgtaaccag caacctcccg gcttgccgga tcattctggg
     1261 aggcgaagga gccgcccgtg tgtcagcgtc agtatgagga atgggccagt ctctgggagg
     1321 cgctgttcgg cgagccgccg cccatcgcgg cggaaatctc gctgaccgcc cggatcctgg
     1381 tggcgtgcct gccgtcagcc gaaccctatg agccggggtc taaaccgcca gcgtcttgag
     1441 ccggtcgcgc agggtgccga ggtcttccag cggcaggtcc agctggcaga ggatcttctc
     1501 ggggatcttc gcggcctggt cgcgcagggc ctgacccctg tctgtcaggg tgatgatcac
     1561 gcggcgctcg tcctggggat cgcgggcgcg ggcgatcagc ccgccggcct ccaggcgctt
     1621 gagcagcggg g
//
LOCUS       DAOMEO010000814         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7686_length_1630_cov_2.450794, whole genome shotgun sequence.
ACCESSION   DAOMEO010000814 DAOMEO010000000
VERSION     DAOMEO010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7686_length_1630_cov_2.450794"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..>1630
                     /locus_tag="PLH31_15690"
     CDS             241..>1630
                     /locus_tag="PLH31_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HQR90671.1"
                     /translation="MRNSLISLASLTLLFASEAMAQDPGASAPVQYEVAFDNASHHEA
                     QISASYANVPAGPLRVQMSRSSPGRYAIHEFAKNVYRVSATDSAGRPLAVERTDPYSW
                     TIAGHDGTVRVTYILYADRGDGTYSQIDTTHAHLNMPATFLWASGYDDKPIRVKFRRP
                     DTTWKVATQLPASSEADTYWAPNLQYFMDSPTEISNFALREWQVTDAGQTYTFRLALH
                     HQGTDADADAFADKLKKILPEHIKVFGELPKFDYGTYTFIADYLPQITGDGMEHRNST
                     VISNTQSLFRANFAQLGTASHELFHAWNVERIRPAELEPFDFTKANPTPSLWLAEGFT
                     QYYGPLLIRRSGQSTVDEYLKALSSTVNGVVNGPGRGYGSPQDMSLRAPFVDAAAALD
                     PTNANIFTSYYPYGAVIGLALDLQLRSRPAPLTLDNYMRRLWLTHGVPETPYKPADLR
                     LALTAVTGDAAFS"
BASE COUNT          321 a          547 c          463 g          299 t
ORIGIN      
        1 gctgcttgac ctacagttat aactagacgt attgttattt tctcgtcgcc tggtcgcggg
       61 ctggagactc cctgcgattg gtaaccccac cccccactga tcgcgagcga tccaggcgac
      121 gagaccttca agccgccagc ccccggcggt aggattgagg tctggtaaac acgccttgcg
      181 accagcgcgt gcgcctctca tggtgcgcgc tatcgccgat acggcagctc cgggggacaa
      241 atgcggaatt ctttgatcag cctggcgagc ctcactctgc tcttcgccag cgaagccatg
      301 gcgcaggatc ccggcgcgag cgcgcccgtg cagtacgaag tggcgttcga caatgcgagc
      361 caccacgaag cgcagatctc cgcgagctac gccaacgttc cggccggacc tttgagggtg
      421 caaatgtcac gctcgtcgcc ggggcgctac gccattcatg aattcgccaa gaatgtctat
      481 cgggtgagcg ccaccgacag cgccggccgt cccctggccg ttgagcggac tgatccgtac
      541 agctggacca tcgccggcca cgacggaact gtacgcgtca cctacatcct ctacgccgac
      601 cgcggggatg gcacctattc gcagatcgac acgacgcatg ctcacctgaa catgccggcc
      661 accttcctgt gggccagcgg atatgacgac aagccgatcc gcgtgaagtt cagacgcccg
      721 gacacgacct ggaaggtggc gacgcagctg ccggcctcaa gcgaggcgga tacctactgg
      781 gcgcccaacc tccagtactt catggacagc cccaccgaga tcagcaactt cgccctgcgc
      841 gaatggcagg tcacagacgc tggccagacc tacacgttcc gtctggccct gcatcatcaa
      901 ggcacggatg cagatgcgga cgccttcgcc gacaagctga agaagatcct ccccgaacat
      961 atcaaggtct ttggcgaact gccgaagttc gactacggga cctacacgtt catcgctgac
     1021 tacttgcctc agatcaccgg cgacgggatg gaacaccgca actccaccgt catcagcaat
     1081 acccaatccc tgtttcgcgc caacttcgcc cagctgggca ccgcgagcca cgagttgttt
     1141 catgcctgga acgtagagcg catacgtcca gcggaactgg agcctttcga cttcacgaaa
     1201 gccaatccga ccccttccct gtggttggcc gagggcttca cccagtatta tgggcctctg
     1261 ctgatccgcc gctcgggcca gtcgaccgtc gatgagtatc tgaaggccct gagttcgacc
     1321 gtgaatggcg tggtcaatgg tccagggcgg ggctatggct cgccccagga catgagcttg
     1381 cgggcgccgt ttgtcgatgc agcagccgct ctcgacccga ccaacgcaaa catcttcacc
     1441 tcctactacc cctatggtgc ggtgattggg ctggcgctgg acctgcagct gagaagccgc
     1501 cccgcccccc tgactctgga caactacatg cgccggctct ggctgaccca cggggttccg
     1561 gagacgccat acaaacctgc tgatctgcgc cttgcattga cggccgtaac gggggatgcg
     1621 gccttctcgg
//
LOCUS       DAOMEO010000815         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7687_length_1630_cov_2.400635, whole genome shotgun sequence.
ACCESSION   DAOMEO010000815 DAOMEO010000000
VERSION     DAOMEO010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7687_length_1630_cov_2.400635"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..>540
                     /locus_tag="PLH31_15695"
     CDS             169..>540
                     /locus_tag="PLH31_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQR90672.1"
                     /translation="MSAEPRQQLFAVDGLFCGGCARGLERRLGETPGVLLARVHHLSA
                     SALVRWEAQRCDVAYLRTVVRQAGYRLIERADPKDAVDRFDGQIRQLTLRLAVSVAFG
                     MWAMVAAIVLYVTPSLGADLAW"
     assembly_gap    541..550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <551..>1630
                     /locus_tag="PLH31_15700"
     CDS             <551..>1630
                     /locus_tag="PLH31_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR90673.1"
                     /translation="SGLLALPALLYGGWDIGRMAIRSVRLRAPGLDLLVTLGVGGAVS
                     ASLVHLMAGDRHVYFDTAVMLITLLLTGRLIEIHIRRGAAQAVVAMGRLFDDTALVWR
                     EADGWQAAPSASLRLGDRVRIPAGAVATVDGRVVTGESRLNAAVLNGESAPRAVTPGD
                     RVSAGAVNLDRMIEVSVDRDAGDRDVDRMGGRVALELATRGGDEDRIARLAGTLTQGL
                     ILAAVLVTAATLAFTADVGEAVMRGLVVLLAACPCALALAAPLAQARLSAKAAEHGVR
                     FSDPGVVDRMTGLRNVFLDKTGTLTLGRPEVVAVTPCPGWSAAQVLDLAARAETGVAH
                     PLAQAVVAAARIGDANGDGLVLVLE"
BASE COUNT          204 a          565 c          580 g          271 t
ORIGIN      
        1 cgccgccttc atcaacagcc aagagcggcc caaggacccc cgccagaccg gcacagtgca
       61 ggagaccgct caaaagaacc acggcggcga ggagacgttt tatgggcgga cctaccaggg
      121 ccgtctgatc ggggtgggca cgcctgaacc gaccacccgc gctcgacctt gagcgcggaa
      181 ccgcgtcagc aactgttcgc cgtcgatggc ctcttttgcg gcgggtgcgc gcgcggtctg
      241 gagcgtcggc ttggcgaaac ccccggcgtc ctccttgcac gggtccatca tctctccgcc
      301 tcggccctgg tccgatggga agcccagcgc tgcgacgtcg cttatcttcg aaccgtcgtc
      361 cgtcaggccg gctatcgcct gatcgagcgc gccgacccca aggatgcggt ggaccggttc
      421 gacggtcaga tccgccagtt gacgctcagg ctcgccgttt ccgtcgcctt cggcatgtgg
      481 gcgatggtcg cggcgatcgt gctctatgtg acgccgagcc taggggcgga tctcgcctgg
      541 nnnnnnnnnn cagcggtctg ctggccctgc cggcgctcct ctatggcggt tgggacatcg
      601 gccgcatggc gatccgatcc gtgcgcctgc gcgcgcccgg gctcgacctc ctggtcacgc
      661 tcggcgtcgg cggcgccgtg tcggcctcgc tcgttcatct gatggccggc gatcgtcacg
      721 tctatttcga cacggcggtc atgctgatca cgctgctgct gaccggacgt ttgatcgaga
      781 tccacatccg gcgcggggcc gcccaggccg tggtggccat gggccgtctg ttcgatgaca
      841 cggccctggt ctggcgggaa gcggacggct ggcaggcggc gcccagcgcc agccttcgcc
      901 tcggcgaccg cgtgcggatc cccgccggcg ccgtggcgac cgtcgacggc cgggttgtga
      961 ccggcgagag ccgtctgaat gcggccgtgc tgaacggcga gtccgcgccg cgcgccgtca
     1021 ctcccgggga tcgcgtcagc gcgggggccg tgaacctcga ccggatgatc gaagtctcgg
     1081 tcgatcgcga cgccggggat cgcgacgtgg atcggatggg cggacgggtg gccctcgaac
     1141 tggcgacgcg cggcggtgac gaggaccgga tcgcgcgcct ggccggcacg ctgacccagg
     1201 gcctgatcct ggcggccgtc ctggtgacgg cggcgacgct ggcgttcaca gccgacgtcg
     1261 gcgaggcggt gatgcgtggt ctggtcgtgc tcctggcggc gtgcccctgc gccctggccc
     1321 tggccgcgcc cttggctcag gcgcggctct cggccaaagc cgccgaacac ggtgtgcggt
     1381 tctccgatcc cggcgtggtc gatcgcatga ccgggctgcg caatgtgttt ctggacaaga
     1441 ccgggaccct caccctggga cgccccgaag tggtcgccgt cacgccctgc cccggctggt
     1501 ccgcagcgca ggtgctggat ctggcggcgc gtgcggagac cggcgtcgcc catcccctcg
     1561 cgcaggccgt cgtcgccgcc gcgcggatcg gcgacgccaa tggtgatggc cttgttctgg
     1621 tccttgagat
//
LOCUS       DAOMEO010000816         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7699_length_1628_cov_2.267006, whole genome shotgun sequence.
ACCESSION   DAOMEO010000816 DAOMEO010000000
VERSION     DAOMEO010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_1628_cov_2.267006"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="secD"
                     /locus_tag="PLH31_15705"
     CDS             <1..1129
                     /gene="secD"
                     /locus_tag="PLH31_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQR90674.1"
                     /translation="AKADAAMAVEQSIETIRRRIDSLGTKEPTITRQGTDRIVIQAAG
                     ESDPEKLKSVIGKTAKLTFQMVDEGVTPEDMAAGRIPPGSEMLPSEVVRKRAVVSGDE
                     LTDSRQTFDSQSGNPVVSFNFNGQGARKFGDATARNIGKKFAIVLDGKIISAPVINGA
                     ITGGSGIITGNFTPESAAELALLLRSGALPAPLNVEEQRTVGAELGADAVKAGAISTL
                     VAFITIVVFMLLAYGLLFGGIAVIALLINGMMIVAAMSITQATLTLPGIAGLILTLAV
                     AVDANVLIYERMRDEARAGKSPIMAADAGFSRAMSTIIDANVTTLAAAAIMFAFGAGP
                     VRGFAWTLSIGVFTSVFTAVLVSQLLIGWWFRAARPKKLPI"
     gene            1130..>1628
                     /locus_tag="PLH31_15710"
     CDS             1130..>1628
                     /locus_tag="PLH31_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQR90675.1"
                     /translation="MSAPMAWPLIKLLPQKTNLTFVKFARLMALISVTLVVASLIGVF
                     KPGLNMGIDFKGGSVMEVSTAPRAVDLAKVRTTLEAQGLGDVQVQEFGSPDRALVRFQ
                     TPAGGDAGVTLTNVKNALKQSLGDPVQFINPTAVGPKVSGELFRSGIIALALAIGLMF
                     LYIWFR"
BASE COUNT          266 a          565 c          518 g          279 t
ORIGIN      
        1 cgccaaggcc gacgcggcga tggcggtgga gcagtcgatc gagaccatcc gtcgccggat
       61 cgacagcctg ggcaccaagg aaccgacgat cacccgtcag ggcacggacc ggatcgtcat
      121 ccaggcggcc ggcgaaagcg atcctgaaaa gctgaagtcg gtgatcggca agaccgccaa
      181 gctgaccttc cagatggtcg acgagggcgt cacgcctgaa gacatggccg ctggccgcat
      241 cccgccgggc tcggaaatgc tgcccagcga ggtggtgcgc aagcgcgctg tggtctcggg
      301 cgacgagctg accgattcgc gtcagacctt cgactcccag agcggcaacc cggttgtctc
      361 gttcaatttc aacggccagg gcgcgcgcaa gttcggcgac gccacggccc gcaacatcgg
      421 caagaagttc gccatcgtcc tggatggcaa gatcatctcg gcgccggtca tcaacggcgc
      481 gatcacgggc ggctcgggca ttatcacggg caacttcacg ccggagagcg ctgctgaact
      541 ggccctgctg ctgcggtcgg gggctctgcc cgcgccgctg aacgtcgagg aacagcgcac
      601 cgtcggcgcc gaactgggcg cggacgcggt caaggcgggg gcgatctcca ccctggtggc
      661 cttcatcacc atcgtggtct tcatgctgct ggcctatggc ctgctgtttg gcggcatcgc
      721 cgtgatcgcc ctgctgatca acggcatgat gatcgttgcg gccatgtcga tcacccaggc
      781 caccctgacc ctgccgggta tcgccggcct gatcctgacc ctggccgtgg ccgtcgacgc
      841 caacgtgctg atctatgagc ggatgcgtga cgaggcccgg gccggcaagt cgccgatcat
      901 ggccgccgac gccggcttct cccgcgccat gagcacgatc atcgacgcca acgtcaccac
      961 cctggcggcg gcggcgatca tgttcgcgtt cggcgcaggc ccggtccggg gctttgcctg
     1021 gaccctgtcg atcggcgtgt tcacctcggt gttcaccgcc gtgctggtca gccagcttct
     1081 catcggctgg tggttccgcg ccgcccgccc caagaaactt ccgatctgag tgagcgcacc
     1141 catggcctgg cccctgatca agctgctccc gcagaaaacc aacctcacct tcgtcaagtt
     1201 cgcgcgcctg atggcgctga tcagcgtcac cctggtggtc gcctcgctga tcggcgtctt
     1261 caagcccggg ctcaacatgg gcatcgactt caagggcggt tccgtcatgg aagtctcgac
     1321 cgccccgcgc gcggttgacc tggccaaggt gcgcaccacg ctcgaagccc agggtctggg
     1381 cgacgtgcag gtgcaggaat tcggctcccc cgaccgcgcc ctggtgcgct tccagacacc
     1441 cgcgggcggc gacgccgggg tgaccctgac caacgtcaag aacgccctga aacagtcgct
     1501 gggcgacccc gtccagttca tcaatcccac cgcggttggt cccaaggttt cgggcgagct
     1561 cttccgcagc ggcatcatcg ccctggcgct cgcgatcggc ctgatgttcc tctacatctg
     1621 gttccggt
//
LOCUS       DAOMEO010000817         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7703_length_1627_cov_2.891221, whole genome shotgun sequence.
ACCESSION   DAOMEO010000817 DAOMEO010000000
VERSION     DAOMEO010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7703_length_1627_cov_2.891221"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLH31_15715"
     CDS             complement(<1..292)
                     /locus_tag="PLH31_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90676.1"
                     /translation="MTVTFRRALMLGAAALCLSSAAQAAEADQTHLDSVLVTARPDPE
                     DPPAVAQARKRLSETPGAVAVISSEAYARRFALGLNDTVRDVPGVFAQKKFGE"
     gene            complement(360..755)
                     /locus_tag="PLH31_15720"
     CDS             complement(360..755)
                     /locus_tag="PLH31_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HQR90677.1"
                     /translation="MIPRRARSGPTLLVALLAALAVLAQTLVPAVAMARSTSAGEAII
                     ICTAEGAKLVKVGGEEPSPSKIFGGFKCGQCLVASLAAITPDPAATIVPVRYAVRLEV
                     AIVRTLGVRGHARAPPRPPGQGPPLLLNA"
     gene            complement(908..>1627)
                     /locus_tag="PLH31_15725"
     CDS             complement(908..>1627)
                     /locus_tag="PLH31_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQR90678.1"
                     /translation="GKSIAALIIVLAFRRPMSTALTISASLAQIGEFSFILAGLGVSL
                     KLLPEAGRDLVLAGAILSILVNPLLFVILDRVLPRMMADEREAAGEPPLIAAAQPHAL
                     LVGYGRVGKRVAEGLKGRTPLVVIEDDPERAAELRAAGYETVNGNAVLPEVLLKAGLD
                     KATHLFVAVPSPFEAARIIEQARAANPKAEIIARAYTDADVELLKSMGATHALVGEDE
                     IAKGMLARAPKKKLAAAAGGH"
BASE COUNT          256 a          520 c          611 g          240 t
ORIGIN      
        1 cctcgccgaa cttcttctgg gcgaacacgc cgggcacatc gcggacggtg tcgttcaggc
       61 ccagggcgaa gcggcgggca taggcttccg acgagatcac cgccacggcc ccgggggttt
      121 ccgacaggcg cttgcgggcc tgggccacgg cgggcgggtc ttcgggatcg ggacgggcgg
      181 tgaccagcac ggagtcgagg tgggtctggt cggcttcggc ggcttgagcg gcggaagaca
      241 ggcaaagggc tgcggcgccc agcatgaggg cgcggcggaa ggtgacggtc atggaaaggg
      301 aagtcctgaa aacgtgacga gacgcgcggg cggcagggcc gtggcgcggg ctttttaggt
      361 caggcgttca ggaggagcgg tggtccctgt ccgggaggac gcggcggcgc gcgggcatgg
      421 ccgcgaacgc ccagggtgcg cacgatggcg acctcaagac ggacggcgta gcgcacggga
      481 acgatggtgg cggccggatc aggcgtgatg gccgccaggc tggcgaccag gcactggccg
      541 cacttgaagc cgccaaagat cttgctgggc gacggctcct cgccgcccac cttgaccagc
      601 ttggcgccct cggcggtaca gatgatgatc gcctcgcccg cgctggtcga gcgggccatg
      661 gcgaccgccg gaaccagggt ctgggccagc accgccagcg ccgccaacag ggcgacgagc
      721 agggtgggac cagatctggc gcgacggggg atcatggctt ggctgactag ggcgtggcga
      781 cgggctggtc aatcagcgcc ggtgtcgcgc gcgctgggat ggctacttaa gggccttcag
      841 aagacaaagc agactgtcat ccgctttcct tccccccgcg cgaaggaagg atctttgaca
      901 ttcgcgccta gtgcccgccc gccgccgccg ccagcttctt cttcggcgcc cgcgccagca
      961 tgcccttggc gatctcgtcc tcgccgacca gggcgtgggt ggcgcccatg cttttgagca
     1021 gttcgacatc ggcgtcggta taggcccggg cgatgatttc ggccttggga ttggcggccc
     1081 gggcctgctc gatgatccgt gccgcctcga aggggctggg cacggcgacg aacaggtggg
     1141 tggccttgtc caggccagcc ttcagcagca cctcggggag caccgcattg ccgttgaccg
     1201 tctcgtagcc ggcggcgcgc agttcggcgg cgcgttcggg atcatcctcg atcaccacca
     1261 ggggcgtgcg gcccttcagc ccctcggcca cccgcttgcc gacccggcca tagccgacca
     1321 gcagggcgtg gggctgggcg gcggcgatca ggggcggctc gccggcggct tcgcgttcgt
     1381 cggccatcat cctgggcagg accctgtcga ggatcacgaa caggagcggg ttgaccagga
     1441 tcgacaggat ggcgccggcc agcaccaaat cgcggccggc ttccggcagc agcttcaggc
     1501 tcacgcccag gccggccaga atgaacgaga actcgccgat ctgggccaga ctggccgaga
     1561 tggtcagggc cgtgctcatc gggcggcgga aggccagcac gatgatcaag gcggcgatcg
     1621 acttgcc
//
LOCUS       DAOMEO010000818         1624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7719_length_1624_cov_2.767368, whole genome shotgun sequence.
ACCESSION   DAOMEO010000818 DAOMEO010000000
VERSION     DAOMEO010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_1624_cov_2.767368"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..929)
                     /locus_tag="PLH31_15730"
     CDS             complement(177..929)
                     /locus_tag="PLH31_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQR90679.1"
                     /translation="MNDFNRGYARAIPADRADMSVDAGLRSFMLGVYNKVALGLLLSA
                     GLAFLTSAYQPVASLLYVLTPDGRLAGFTMAGTIVRFAPLAMMLVAMFAMKNPTAKSS
                     GALYWALVASIGAGAGVWLLAYTGASVASTFLITAIAFGGLSLFGYTTKKDLTGFGSF
                     LIMGLIGLVLASLAQMFLKLPGLSFIISILGVFIFAGLIAYDTQKLKTAYYEMGGDQN
                     SMAVATNYGALNLYLNFINLFQFLLSLFGARR"
     gene            1079..>1624
                     /locus_tag="PLH31_15735"
     CDS             1079..>1624
                     /locus_tag="PLH31_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR90680.1"
                     /translation="MDYSELGRTGMMVSRCCLGTMTWGSQNSEDEAHAQMDYALERGV
                     NFWDTAEMYSSPPDPRTQGNTERQIGSWLKKTGKRDEIVLASKVAGRGSAFGGLTWMR
                     ADGAPTRHTKAQIDEAVEGSLKRLNTDYLDLYQLHWPDRPVRVFGGQTFKDYEQDYET
                     FGDILEALDAHVKKGTIRAIGV"
BASE COUNT          333 a          501 c          542 g          248 t
ORIGIN      
        1 gcgatcacgg tcaggccggc cagaagagtc agggtgcggg tcatgaaatc ctcaaagcgg
       61 ctcttcttgt ccccgccaac aaggtggcgc cgaaggtcgg cggcgtcgag ggtgatccac
      121 gaaaaagccc cggcagtttc ccgccggggc ttcgctgttt cgtccgggct tgggttctag
      181 cggcgagcgc cgaagaggct gagcaggaac tggaacaggt tgatgaagtt cagatacagg
      241 ttcagcgcgc catagttggt ggccaccgcc atcgagttct ggtcgccgcc catttcataa
      301 taggcggtct tcagcttctg ggtgtcgtag gcgatcaggc ccgcgaagat gaacacgccc
      361 aggatgctga tgatgaagct caggcccggg agcttgagga acatctgggc cagcgacgcc
      421 agaaccaggc cgatcaggcc catgatcagg aagctgccga aacccgtcag gtccttcttg
      481 gtggtgtagc cgaacaggct gagaccgccg aacgcgatgg cggtgatcag gaaggtcgaa
      541 gccaccgaag cgccggtata ggccagcagc cagacgcccg cgcccgcgcc gatcgaggcg
      601 accagcgccc agtacaaggc gcccgacgac ttggcggtag ggttcttcat cgcgaacatg
      661 gccaccagca tcatcgccag cggggcgaag cgcacgatcg tgccggccat ggtgaagccg
      721 gccaggcgac cgtccggggt cagcacatag agcaggctgg ccaccggctg gtaggcgctg
      781 gtcaggaagg cgaggccggc ggacagcagg aggcccaagg ccaccttgtt gtagacgccg
      841 agcatgaagc tgcgcaggcc ggcgtcaacc gacatgtcgg cgcgatccgc cgggatcgcg
      901 cgcgcatagc cgcggttgaa gtcgttcatc gagggaaagc ccttttgatt gcatcacgcc
      961 cagattggac gccccacgaa tatcggcatc ctttggcctt cgcgcaaggc atggctggcg
     1021 agacccgcgc cggaccgctt aaatagaggc cagcgcaaat caggatgggt cgaacggcat
     1081 ggattattcg gaactggggc gcacgggtat gatggtctcg cgctgctgcc ttggcaccat
     1141 gacctggggc tcgcagaaca gcgaagacga ggcccacgcc cagatggact atgcgctcga
     1201 gcgcggcgtc aatttctggg acacggccga gatgtattcc agcccgcccg accccaggac
     1261 ccagggcaat accgagcgcc aaatcggctc atggctgaaa aagaccggca agcgcgacga
     1321 gatcgtgctg gcctccaagg tggccggtcg cggcagcgcc tttggcggcc tgacctggat
     1381 gcgcgccgac ggcgccccca cccgccacac caaggcccag attgacgagg ccgtcgaagg
     1441 ctcgctcaag cgcctgaaca ccgactatct ggacctctac cagctgcact ggcccgaccg
     1501 gccggtgcgg gtgttcggcg gccagacctt caaggactat gagcaggact acgaaacctt
     1561 cggcgacatc cttgaggcgc tggacgccca tgtgaagaag gggacgatcc gcgccatcgg
     1621 ggtc
//
LOCUS       DAOMEO010000819         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7729_length_1623_cov_2.919005, whole genome shotgun sequence.
ACCESSION   DAOMEO010000819 DAOMEO010000000
VERSION     DAOMEO010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7729_length_1623_cov_2.919005"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLH31_15740"
     CDS             <1..277
                     /locus_tag="PLH31_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HQR90681.1"
                     /translation="VAAVSGDQVCTGDGRLVINGQVRAPILNKDRRGAVLPRWNGCRR
                     LLDGEVFVFSDRIPNSFDSRYFGPVNRAEIVGVFRPVTLSLSHPGDR"
     gene            427..1110
                     /locus_tag="PLH31_15745"
     CDS             427..1110
                     /locus_tag="PLH31_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HQR90682.1"
                     /translation="MAGPDAREAITRVAFAEAGNQGDSGLAAVVYTILNRVQDGRWGA
                     TVEAVVNARGQFEPVMRVGGDWRRLPASTVAQRARIETILNLALEGRLPDLTNGARFF
                     QNPQIVADRATRGEVSEGLVNFGGTGPSAVIGAHSFYVDVRSAGGRGTRSAKPSSTPR
                     GSSSAAAWQSAGGAIFVGDNRAAQDPSAVSSPAPEAAGQGAMRATKVADPGRSIFVDG
                     PGLAAGEDR"
     gene            complement(1111..>1623)
                     /locus_tag="PLH31_15750"
     CDS             complement(1111..>1623)
                     /locus_tag="PLH31_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90683.1"
                     /translation="ETALREAGQDGIHKQRHVTPLEDIEDDAKRFHVLKARVERLEAL
                     WSINKRRRETRGKIIIGGALLAEAGDAHFEGDDELLARLVDILDRRVERVRDRLTVRE
                     LLGDVPLPLRPGGDVNEDAQSALQAAGEPLPDFDLMAESALAQEAGGELLPSEVDPDY
                     ADLDPAWRAA"
BASE COUNT          230 a          570 c          545 g          278 t
ORIGIN      
        1 cgtcgccgcc gtgtccggcg accaggtctg cacgggcgac ggccggttgg tgatcaatgg
       61 acaggtgcgc gccccgatcc tgaacaagga tcgccgcggc gctgtcttgc cgcgttggaa
      121 cggctgccgc cggcttctcg acggcgaagt gttcgtcttc tccgaccgca tccccaacag
      181 cttcgacagt cgctacttcg gcccggtaaa ccgcgccgaa atcgtcggtg tgttccggcc
      241 ggtgaccctg tcgctttccc accctggaga ccgttgatgg acccggctgc cctggcggga
      301 ttgtgcctgc ttctcgcgcg cggacagagc gcccaaccgt tgcccgcggg cgtcaattgc
      361 ccgccacccg cgcccgtctc ggcggtgatg gcgccgcctt tgacgcggag cggtcttgct
      421 cccctcgtgg ccggcccgga cgcgcgcgaa gcgatcaccc gggtcgcctt cgccgaggcc
      481 ggtaatcagg gcgacagcgg cctggccgcc gtcgtctaca cgatcctcaa ccgggtccag
      541 gacgggcgat ggggcgccac ggtcgaggcc gtggtcaatg cccgaggcca gttcgagccg
      601 gtgatgcggg tcggcggcga ctggcgtcgc ctgccggcct ccacagtcgc gcagcgggcg
      661 cggatcgaga ccattctcaa tctcgctctt gagggtcgcc tacctgatct caccaacggg
      721 gcgcgcttct tccagaaccc gcaaatcgtg gcggaccggg cgacccgcgg cgaggtctcc
      781 gagggcctcg taaacttcgg cggaacgggg ccttcggccg tgatcggcgc ccatagcttc
      841 tatgtcgatg tgcgctcggc cggggggagg ggcacgcgtt ccgccaagcc ctcgtccacc
      901 cctcgtggat cttcctcagc agcggcctgg cagtccgccg gcggggcgat cttcgtcggc
      961 gacaatcgcg ctgcgcaaga cccgagcgca gtctccagcc cagccccgga agcggcaggg
     1021 cagggggcta tgcgcgcgac gaaagtcgcc gaccccggcc gctccatctt cgtggacggc
     1081 ccgggcctgg cggccggaga agaccgatga tcaggcggcc cgccaagccg ggtcgagatc
     1141 ggcgtagtcc gggtcgactt cgctcggcag cagctccccg ccggcttcct gagcgagggc
     1201 agattcggcc atcagatcga agtccggcag gggctctccg gcggcctgga gcgctgactg
     1261 tgcgtcttca tttacgtcgc cgccaggccg caagggcagg ggcacatcgc ccagaagttc
     1321 gcgcacggtc aggcggtcgc gaacccgttc tacacgccgg tcgaggatat cgaccaggcg
     1381 cgccagaagc tcgtcgtcgc cttcgaaatg cgcgtcgcct gcctcggcca gcagggcgcc
     1441 gccaatgatg attttgccac gtgtttcgcg tctgcgtttg ttgatcgacc agagcgcttc
     1501 aagccgttcg acgcgcgcct tcagcacgtg gaaacgcttg gcgtcatcct cgatatcttc
     1561 aagcggggtg acgtgccgct gcttatggat tccgtcctgg cccgcctctc gcagcgccgt
     1621 ctc
//
LOCUS       DAOMEO010000820         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7738_length_1622_cov_2.641991, whole genome shotgun sequence.
ACCESSION   DAOMEO010000820 DAOMEO010000000
VERSION     DAOMEO010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7738_length_1622_cov_2.641991"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..903)
                     /locus_tag="PLH31_15755"
     CDS             complement(109..903)
                     /locus_tag="PLH31_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR90684.1"
                     /translation="MLLINNIEVVYDRVILVLKGVSIDVREGAITTLLGANGAGKTTT
                     LKAISGVLHGERGEVTKGTIQIGDRRIDGMRAHDVTRLGLAQVFEGRRVFEHLTTEEN
                     LIAGGHVQKDGGAVKQGIELVYTYFPRLRERRHQQSGYLSGGEQQMLAIGRALMSKPR
                     VVLLDEPSLGLAPMLVEEIFGIVSRLVQQEKLSVLLVEQNAAMALAVADHGYVMENGR
                     IVLEGPADKLRDNSDIKEFYLGLNEGGARKSYRDTKHYKRRKRWLS"
     gene            complement(944..>1622)
                     /locus_tag="PLH31_15760"
     CDS             complement(944..>1622)
                     /locus_tag="PLH31_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQR90685.1"
                     /translation="DLAKSEGFEMRTFAVPAPGVEMGAQILDITGRFKPDFIITHLFG
                     RSPSVSIKELKGKGFPLSKVVAFVWGSAEADIIAAGGMGVAEGYNGIQFAGVGKDFQV
                     IKDIEAMYKAQGKPVPKELDSTVYYNRGVFIAALHTEAVRNAIKAKGGAAPTSEEVKK
                     GMESIKGFTLGGLVPPMEITPTDHEGGGWVQIWTVKGGKLVKSKDWFQGFRPVIQKHL
                     EAEAKKS"
BASE COUNT          270 a          524 c          488 g          340 t
ORIGIN      
        1 gcggcgcgct cgttcctgag cgattgggcc cacggtatct tgcattgacc tggcgcttcg
       61 cgttcaaggg tcccatcgtc caatactttc acgacgcgca atagaaagtc acgatagcca
      121 gcgcttgcgc ctcttgtaat gcttggtatc gcggtatgac ttgcgcgcac cgccttcgtt
      181 caggccgaga tagaactcct tgatgtcgga gttgtcgcgc agcttgtcag caggtccctc
      241 caggacgatg cggccgtttt ccatgacata accgtggtcg gcgacggcca gcgccatggc
      301 ggcattctgc tcgaccagga gaaccgagag cttctcctgc tgcaccaggc gactgacgat
      361 gccaaagatc tcctcgacca gcatgggggc caggccgagc gagggttcgt cgagcaggac
      421 tactctgggc ttgctcatca gggcccggcc gatcgccagc atctgctgtt cgccgcccga
      481 caggtagccg gactgctgat ggcggcgctc cctcagcctc gggaaatacg tatagaccag
      541 ctcgatgccc tgcttcaccg caccgccatc cttctggaca tggccgccgg cgatcaggtt
      601 ttcctcggtg gtcaggtgct cgaacacgcg ccggccctcg aacacctggg cgaggccgag
      661 ccgcgtgaca tcgtgggccc gcatgccgtc gatacggcgg tcgccgatct ggatggtgcc
      721 cttggtgacc tcgccgcgct cgccgtgcag cacgccggag atcgccttga gcgtcgtcgt
      781 cttgccggcg ccgttggcgc ccagcagcgt cgtgatggcg ccttcacgca cgtcgatcga
      841 cacgcccttg aggacgagga tgacgcggtc gtagacgact tcgatgttgt tgatcagcag
      901 catgggatgt cgggcggcga ttgctcgccg cccgcccgtt cgtttaggat ttcttggctt
      961 ctgcctcgag atgcttctgg attacgggcc ggaagccttg gaaccagtct ttggacttca
     1021 cgagcttgcc gcccttaact gtccagattt ggacccagcc accgccctcg tggtcggtcg
     1081 gcgtgatctc catcggcggc accaggccgc cgagcgtaaa gcccttgatc gactccatgc
     1141 ccttcttgac ctcttccgag gtcggcgccg ctccgccttt ggccttgatc gcattgcgca
     1201 ctgcctcggt atgcagcgct gcgatgaaga caccgcgatt atagtagacg gtcgagtcca
     1261 attccttcgg cacgggcttg ccctgtgcct tgtacatggc ctcgatgtcc ttgatgacct
     1321 ggaagtcctt gccgacgcca gcaaactgga tgccgttgta accttcggcc acacccatgc
     1381 cgccggccgc gatgatatcg gcctcggcgc tgccccacac gaaggccacc actttgctga
     1441 gtgggaaacc cttacccttc agttccttga tggagaccga cggcgagcgt ccgaacaggt
     1501 gggtgatgat gaaatccggc ttgaagcggc cggtgatgtc gagaatctgg gcacccatct
     1561 cgacgccggg cgctggtacc gcaaaagtgc gcatctcgaa gccttcggac ttcgcgagat
     1621 cc
//
LOCUS       DAOMEO010000821         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7745_length_1621_cov_2.977650, whole genome shotgun sequence.
ACCESSION   DAOMEO010000821 DAOMEO010000000
VERSION     DAOMEO010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7745_length_1621_cov_2.977650"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /gene="ilvD"
                     /locus_tag="PLH31_15765"
     CDS             <1..1518
                     /gene="ilvD"
                     /locus_tag="PLH31_15765"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQR90686.1"
                     /translation="VKAAGGTPREFCTITVTDGIAMGHEGMRSSLVSRDCIADSVELT
                     MRGHGYDALVGVAGCDKSLPGMMMAMLRLNVPSVFLYGGSILPGRWGGKDVTVVDVFE
                     GVGMHSAGKMTLEDLCDLETHACPSDGACGGQFTANTMACVSEAIGLALPLSASLPAP
                     YLSRDEYAVASGEAVMRLIESNLRPRDICTRKAFENAAVVVAATGGSTNGGLHLPAMA
                     HECGIEFTLRDVAEIFKRTPYLADLKPGGQFVAKDMGEAGGMPVLLKTLLEGGFIHGD
                     CMTVTGKTMAENLKDVKWRDDQVVIRPVSNPLSPTGGVVGLWGSLAPDGAIVKVAGMT
                     SHRSHRGPARCFEGEDACFDAVAAGDYQDGDVLVIRYEGPKGGPGMREMLSTTAALAG
                     QGRGDKVALVTDGRFSGGTRGLCIGHIGPEAQAGGPIALVQDGDIINIDADAGTIDLE
                     VAPEELARRKAAWSPRKTNYNSGAIWKFAQLVGPAHLGATTHPGADGETHIYADL"
     gene            1530..>1621
                     /locus_tag="PLH31_15770"
     CDS             1530..>1621
                     /locus_tag="PLH31_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR90687.1"
                     /translation="MSHAAWPVDPGMIAPFLVAVILIELTPGPN"
BASE COUNT          253 a          577 c          551 g          240 t
ORIGIN      
        1 gtcaaggcgg ccggcggtac gccgcgcgag ttctgcacca tcaccgtcac cgacggcatc
       61 gccatgggcc acgagggcat gcgctcatcc ttggtcagcc gcgactgcat cgccgactcg
      121 gtggaactga ccatgcgcgg ccacggctat gacgccctgg tcggcgtggc cggctgcgac
      181 aagagcctgc cgggcatgat gatggccatg ctgcgcctga acgtgcccag cgtgttcctg
      241 tatggcggct ccatcctgcc cggccgctgg ggcggcaagg acgtcaccgt ggtcgacgtg
      301 ttcgaaggcg tcggcatgca ctcggccggc aagatgacgc tggaagacct gtgtgacctg
      361 gaaacccacg cctgtccgtc ggacggcgcg tgcggcggcc agttcaccgc caacaccatg
      421 gcctgcgtct ccgaagccat cggcctggcc ctgccgctgt cggcctcgct gccggcgccc
      481 tatctgagcc gcgacgaata tgccgtggcc tcgggcgagg cggtgatgcg gctgatcgag
      541 agcaatctgc ggccgcgcga catctgcacc cgcaaggcgt tcgagaacgc cgccgtcgtc
      601 gtcgccgcca ccggcggctc gaccaatggc ggcttgcacc tgccggccat ggcccacgag
      661 tgcggcattg aattcaccct gcgcgacgtg gccgagatct tcaagcgtac gccctatctg
      721 gccgacctga agccgggcgg tcagttcgtg gccaaggaca tgggcgaggc cggcggcatg
      781 ccggtgctgc tcaagaccct gctggaaggc ggcttcatcc acggcgactg catgacggtg
      841 accggcaaga ccatggccga gaacctcaag gacgtgaaat ggcgtgacga tcaggtggtg
      901 atccgcccgg tctccaaccc gctgtcgccg accggcggcg tggtgggcct gtggggctcg
      961 ctggctcccg acggcgccat cgtcaaggtg gcgggcatga cctcgcaccg cagccatcgc
     1021 ggcccggccc gctgcttcga gggcgaggac gcctgtttcg acgccgtcgc ggccggcgac
     1081 tatcaggacg gcgacgtcct ggtcatccgc tacgaaggcc ccaagggcgg tcccggcatg
     1141 cgcgagatgc tgtcgaccac ggcggccctg gccggccagg gccgcggcga caaggtggcc
     1201 ctggtcaccg acggccgctt ctcgggcggc acgcgcggcc tgtgcatcgg ccatatcggt
     1261 ccggaggcgc aagccggcgg ccccatcgcc ctggtgcagg acggcgacat catcaatatc
     1321 gacgccgacg ccggcaccat cgatctggaa gtcgcgcccg aggaactggc ccgccgcaag
     1381 gccgcctggt cgccgcgcaa gaccaactac aactctggcg ccatctggaa gttcgcccag
     1441 ctggtgggcc cggcccacct gggggccacc acccatccgg gcgcggacgg cgagacccac
     1501 atctacgccg acctctaggg gcaagaggct tgtcccacgc ggcctggccc gtcgaccccg
     1561 ggatgatcgc gcccttcctg gtcgcggtca tcctgatcga actgacgccc gggcccaaca
     1621 t
//
LOCUS       DAOMEO010000822         1620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7754_length_1620_cov_2.313099, whole genome shotgun sequence.
ACCESSION   DAOMEO010000822 DAOMEO010000000
VERSION     DAOMEO010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7754_length_1620_cov_2.313099"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1620
                     /locus_tag="PLH31_15775"
     CDS             60..>1620
                     /locus_tag="PLH31_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90688.1"
                     /translation="MAKGPQSTLFGRGALIGAVNLVQNKADPSGNGFSLKAEGGDYGY
                     LLGEAVGNFAINERTALRLAIRSKSRDGYVDDEAGGEAFNSLRTSAIRAAFHMEPTDR
                     LTIDLIGNWQEDDTTGTSFKSNTFNPTNPVTGAVLGDRGVASGATLSPGAGFENGSPL
                     GLERSVYGLTALTDYRLTDSLSLSSITATRAFHSVEVFDPDGSSLPILTFAEDAQGRQ
                     QSQELRLNYDAGGRFTGFAGVNYFHETGSWRIPGQFDERLVLALLAGNTPLGISRPNP
                     QPLAVLTNPAYSATVLQLGFGIPAALAQGIAANLKPVHREEFANFAENTAWDAYMDGT
                     LKVTDKFELTAGLRYTSEEREASYRSGNTNGRSILGILLAAPTPTNLFLGAQPNAVNS
                     TTLPNIGLITQPTTGNGGLFTRQIEDTGVTWRLVGRYAASDNLSLYGSYARGRRPDTI
                     STSGAAAANGPARFSVIDAETVDSYEAGVKARLMEGRLFLAGSVFQYSYDNFATTFRQ
                     GAQVVTLNAGEA"
BASE COUNT          288 a          582 c          500 g          250 t
ORIGIN      
        1 aagaccgcac ccggcaaggc ataaccaaat cccagcaaac gattaaccca tgctgcgagg
       61 tggccaaggg gccgcagtcg accctgtttg gccgcggcgc cctgatcggg gcggtgaacc
      121 tggtgcagaa caaggccgac cccagcggca acggcttcag cctgaaggcc gagggcggcg
      181 actatggcta tctgctgggc gaagcggttg gcaatttcgc gatcaatgag cgcaccgcgc
      241 tgcgcctggc catccgctcc aagtcgcggg atggctacgt cgatgacgag gcgggcggcg
      301 aggctttcaa ctccctgcgc accagcgcga tccgggcggc cttccacatg gagccgaccg
      361 accgtctgac catcgacctg atcggcaact ggcaggaaga tgacaccacc ggcacctcgt
      421 tcaagtcgaa taccttcaac ccgaccaacc ccgtgaccgg cgccgttctg ggcgatcgcg
      481 gcgtcgcctc gggcgcgacc ctgagccccg gcgcgggctt cgagaatggt tcgccgctgg
      541 gcctggagcg cagtgtctat ggcctgaccg ccctgaccga ctaccgcctt accgacagcc
      601 tcagcctgtc gtcgatcacc gccacgcgcg ccttccactc ggtcgaagtg tttgatccgg
      661 acggctcgtc gctgccgatc ctgaccttcg ccgaagacgc ccagggccgt cagcaaagcc
      721 aggagctgcg cctgaattac gacgccggcg gccgcttcac cggcttcgcg ggcgtgaact
      781 atttccacga gaccggctcg tggcgcattc cgggccagtt cgacgagcgt ctggtcctgg
      841 ccctgctggc gggcaacacc ccgctgggca tcagccgtcc caacccgcag cccctggccg
      901 tgctgaccaa cccggcctat tcggccaccg tgctgcaact gggctttggc attccggccg
      961 cgctggccca aggcatcgcc gccaacctga agccggtgca tcgcgaagag ttcgccaact
     1021 tcgccgagaa caccgcgtgg gacgcctata tggacggcac gctgaaggtc accgacaagt
     1081 tcgaactgac cgccggcctg cgctacacca gcgaagagcg cgaagcctcg taccggtccg
     1141 gcaacaccaa cggccgctcg atcctgggta tcctgctggc ggcgccgacc ccgaccaatc
     1201 tgttcctggg ggcccagccg aacgcggtca acagcaccac cctgcccaat atcggcctga
     1261 tcacccagcc caccacgggc aatggcggcc tgttcacccg gcagatcgaa gacaccggcg
     1321 tcacctggcg tctggtcggt cgctatgcgg cgtcggacaa tctcagcctc tatggctcgt
     1381 acgcgcgggg ccgtcgtccc gacaccatct cgaccagcgg cgcggcggcg gcgaacggcc
     1441 cggcccgctt cagcgtgatc gacgccgaga cggtcgactc ctatgaagcc ggcgtgaagg
     1501 ctcgcctgat ggaagggcga ctgttcctgg ccggttcggt gttccagtac agctacgaca
     1561 acttcgccac gaccttccgc cagggcgccc aggtggtgac cctgaacgct ggcgaagcca
//
LOCUS       DAOMEO010000823         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7766_length_1618_cov_2.557262, whole genome shotgun sequence.
ACCESSION   DAOMEO010000823 DAOMEO010000000
VERSION     DAOMEO010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7766_length_1618_cov_2.557262"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLH31_15780"
     CDS             <1..1166
                     /locus_tag="PLH31_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90689.1"
                     /translation="SRRAMQAERSARLAAEAQTAAKSAFVAMVSHELRTPINALLNGA
                     ATLERQEGADRGAASLIADAGAMMKTLLNDLLDYSKIEAGRMGVEIVDYDLRGLVRDS
                     VLLWRGEARSKGVKLELKGIKALPVWVRGDPMRLRQILNNLLSNAIKFTAEGGVVLTI
                     AMQERAEGRHMVIEVVDTGPGLTEEQISRLFIAYDQLGAGTARAFGGTGLGLAISRDL
                     AGLMGGTLTAASDAGGGARFTLTLPAPEGAAPPSVDLSSPSPLVATAQAPLVLVVDDH
                     DINRRTLRGMLEAFGARVETAEDAESALEAVHARVFDAILMDVRMPGVDGLEATRRLR
                     ASGLNRDTPVIAVTGAASVQDIEACRAAGMTGLVEKPVAPSDLYAALFSQVED"
     gene            complement(1216..1476)
                     /locus_tag="PLH31_15785"
     CDS             complement(1216..1476)
                     /locus_tag="PLH31_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90690.1"
                     /translation="MMLQRKVDYLDAIAPSSRQRPALRLVNETDAPVQSAAHGLQYEI
                     ARVFAQESKWSVRRTVAIGVVFHLGVFCALGLAASGAIGQIR"
BASE COUNT          281 a          493 c          581 g          263 t
ORIGIN      
        1 ggtcgcgacg cgccatgcag gccgagcggt cagcgcggct ggcggccgag gcccagacgg
       61 cggccaagtc ggcctttgtc gccatggtca gccatgagct gcgcacgccc atcaatgcgc
      121 tgctgaacgg ggcggccacg ctggaacgcc aggagggcgc tgatcgcggc gcggccagcc
      181 tgatcgcgga cgccggggcc atgatgaaga ccctgctcaa cgacctgctc gactattcga
      241 agatcgaggc ggggcgcatg ggcgtcgaga tcgtggacta tgacctgcgc gggctggtgc
      301 gcgacagcgt gctgctctgg cggggcgagg cgcggtccaa gggcgtcaag ctggagttga
      361 aggggatcaa ggccctaccg gtctgggtgc ggggcgaccc gatgcggttg cggcagatcc
      421 tcaacaatct gctgtccaac gccatcaagt tcaccgccga gggcggggtg gtgctgacca
      481 tcgccatgca ggagcgcgcc gaaggtcgcc acatggtcat cgaggtggtg gataccgggc
      541 cgggcttgac cgaggagcag atcagccggc tgttcatcgc ctacgatcag ctgggcgcgg
      601 gcacagcgcg ggcctttggc ggtacgggcc tgggtctggc gatcagccgc gatctggccg
      661 gactgatggg cggaaccctg acggcggcca gcgacgctgg cggcggggcc cggttcaccc
      721 tgaccctgcc ggcccccgaa ggcgctgcgc cgccgtccgt cgacctgtcg agtccctctc
      781 cactcgtcgc gacggcgcag gcgcccctgg tccttgtggt ggatgaccac gacatcaatc
      841 gccggaccct gcgcggcatg ctcgaagcgt tcggtgcgcg ggtcgagacg gcggaggacg
      901 ccgagagcgc gctggaagcg gtccatgccc gcgtcttcga cgcgatcctg atggatgtgc
      961 ggatgccggg cgttgacggg ctggaggcca cccggcggct gcgcgcttcg gggctgaacc
     1021 gagatacgcc agtgatcgcg gtgaccggcg cggcgtcggt ccaggacata gaggcctgcc
     1081 gagccgctgg gatgacgggg ctcgtcgaga agccggtggc gcccagcgat ctctacgccg
     1141 ccctgttcag ccaggtggaa gactagaggc tgacccccaa ccctttccag aggggaaggg
     1201 ttggggcgcg ttttgtcagc ggatctggcc gatagcgccg ctggcggcca ggcccagggc
     1261 gcagaagacg ccaaggtgga acacgacgcc gatcgccacg gtgcggcgaa cagaccactt
     1321 ggactcctga gcaaagaccc gggcgatctc gtattgcagg ccgtgagccg cgctctgaac
     1381 cggcgcatca gtttcgttga cgaggcgaag ggcagggcgc tgacgcgagc tcggtgcgat
     1441 ggcgtccaga tagtccactt tacgctgaag catcactgac ctccttatgt gtttcaaggc
     1501 acagtagaga gatcaagcta acaaactatg ctcatcgatt tgggcttgga ttgcgcatgt
     1561 ctggaaaaag cccgaattgg gtatatttag actgcattaa gcacgtctat cttcaatc
//
LOCUS       DAOMEO010000824         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7768_length_1618_cov_2.419706, whole genome shotgun sequence.
ACCESSION   DAOMEO010000824 DAOMEO010000000
VERSION     DAOMEO010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_1618_cov_2.419706"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLH31_15790"
     CDS             <1..745
                     /locus_tag="PLH31_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90691.1"
                     /translation="DHFAPVRASIAKRVLTELNGVRRLKPNDAEAEIEFLRALAMSLT
                     AAAGRILPPEDIQAAFTVRSKTLLNGEFIEALLGRDRSAREEITILIRLAENVMGAVN
                     KRQAARWLSANITALRFEKELRQGPDSPASKLAALAGLQKTVARSGLVREDYEPLCAK
                     LGELGAAVEADSRLLTMLVRAPAPLAHRLALLVKLAMGEAGPTGPVADKAKIEAFKLA
                     RDPAAREELAASPATMDLLKGLMQQAQAA"
     gene            837..>1142
                     /locus_tag="PLH31_15795"
     CDS             837..>1142
                     /locus_tag="PLH31_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HQR90692.1"
                     /translation="MVIFSVLYAATDGAHFDHDYYNATHIPLVHEAFGETGLTGVQVF
                     KGLSAPDGGPAPFIAMAHLAFRDAEALQASMGGPRAAEVMADVAKFTNIQPIAQVSGL
                     "
     assembly_gap    1143..1152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1500..>1618)
                     /locus_tag="PLH31_15800"
     CDS             complement(1500..>1618)
                     /locus_tag="PLH31_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQR90693.1"
                     /translation="LPGAQAWYDRCLTLPLYPTMADGDVERVVEALGKVLAR"
BASE COUNT          257 a          577 c          527 g          247 t
ORIGIN      
        1 cgaccatttc gcccccgttc gcgcctcgat cgccaagcgg gtgctgaccg aactgaacgg
       61 cgtgcgccgg ctgaaaccga acgacgccga ggccgagatc gaatttctcc gcgccctggc
      121 catgagcctg accgccgccg ccggccgcat cctgccgccc gaagacatcc aggcagcctt
      181 caccgtccgc tccaagaccc tgctcaatgg cgagttcatc gaggccctgc tgggccgcga
      241 ccgctcggcc cgcgaggaga tcaccatcct gatccgcctg gccgagaacg tcatgggcgc
      301 ggtcaacaag cgtcaggccg cccgctggct cagcgccaac atcaccgccc tgcgcttcga
      361 gaaggaactg cgccagggtc ctgattcacc ggcctccaag ctcgccgccc tggccggcct
      421 gcagaagacc gtcgcgcgct ctggcctcgt gcgcgaagac tatgagcccc tgtgcgccaa
      481 gctgggcgaa ctgggcgccg cggtcgaggc cgacagccgc ctgctgacca tgctggtccg
      541 cgcgccggcc cccctggctc accggctggc cctgctggtc aagctggcca tgggcgaggc
      601 cggccccacc ggccccgtgg ccgacaaggc caagattgaa gccttcaagc tggcccgcga
      661 tccggcggcg cgcgaggaac tggcggcctc tccagcgacc atggacctgc tcaagggcct
      721 gatgcagcag gcccaggccg cctgacgtcc gcctagtcgg cttgcgacgc gcctgaagac
      781 ccttaagttg cgatgcaacg acgccgccat ggcgtcgatg gcacaagggc ggcgacatgg
      841 tgatcttcag cgttctttac gcggcgaccg acggcgcgca tttcgatcat gactattaca
      901 acgccaccca cattccgctc gtccacgagg ccttcggcga aacggggctg acgggcgttc
      961 aggtcttcaa ggggctgtcg gcgccggatg gcggccccgc gcccttcatc gccatggcgc
     1021 atctggcgtt ccgcgacgcc gaggcgctgc aggcgtcgat gggcggaccg cgcgcggccg
     1081 aggtgatggc cgatgtggcc aagttcacca acatccagcc gatcgcgcag gtcagcgggc
     1141 tgnnnnnnnn nncgacattc gagaccttgg gattgaagcc cgtttgcagg gctcggtcgt
     1201 tcccgcctga aaacgtggat attccgacgc ttgcaacggt cggccggcac cagaagcaga
     1261 cgtagtgact gtccgccacg cgtggattgc tgccttggcg ccgggacgcc ccctccgtca
     1321 cgtcgcctat cggcgccgcg ccacctcccc cgcacgcttc gctacggggc aggagggagc
     1381 gctcttttcc tgccccgtga aacgggggag gtggcgcgct gcgaatacgc agcgtgacgg
     1441 agggggcgtc cggtgtcggc cgttgaacct ccgacgtggg tcggaaagcg aactgcccgt
     1501 tagcgcgcca gcaccttgcc cagcgcctcg accacccgct cgacgtcgcc gtcggccatg
     1561 gtcggatata gcggcagggt caggcagcgg tcgtaccagg cttgcgcgcc cggaaggt
//
LOCUS       DAOMEO010000825         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7771_length_1618_cov_2.156110, whole genome shotgun sequence.
ACCESSION   DAOMEO010000825 DAOMEO010000000
VERSION     DAOMEO010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7771_length_1618_cov_2.156110"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PLH31_15805"
     CDS             complement(<1..134)
                     /locus_tag="PLH31_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQR90694.1"
                     /translation="MKPVFEITKAAHFDAAHYIEQGPADHRYRKLHGHSFKVEASVKG
                     P"
     gene            complement(208..840)
                     /gene="queE"
                     /locus_tag="PLH31_15810"
     CDS             complement(208..840)
                     /gene="queE"
                     /locus_tag="PLH31_15810"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HQR90695.1"
                     /translation="MTYSVKEIFLTLQGEGGQAGKAAVFCRFAGCNLWSGREQDRHKA
                     VCAFCDTDFVGTDGQGGGKFATAELLADAVEAAWTGGPGDRLVVCTGGEPLLQLDSPA
                     IAALHARGFMIAVESNGTLAAPDGIDWICVSPKADAPVAQTRGQELKLVYPQEKALPE
                     RFENLDFERFYLQPMDGPDRDANTQLAVAYCLSHPQWRLSVQTHKYLGLP"
     gene            complement(837..1553)
                     /gene="queC"
                     /locus_tag="PLH31_15815"
     CDS             complement(837..1553)
                     /gene="queC"
                     /locus_tag="PLH31_15815"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQR90696.1"
                     /translation="MTDAALVLFSGGQDSSVCLAWALERYGRVETVGFDYGQRHVIEM
                     EARVAVRHQIAARFPQWAERLGEDHVLDLRGFGAVAESALTAERAIEMTDRGLPSTFV
                     PGRNLVFLTYAAALADRRGIAALVGGMCETDFSGYPDCRRDTLDAMQTALNLGMDRDF
                     RIETPLMKLTKAQTWALAKSLGGEDLVDLIVVESHTCYRGERGDLHPWGHGCGDCPAC
                     DLRQKGYEDWDAAGRGVLAP"
BASE COUNT          280 a          540 c          525 g          273 t
ORIGIN      
        1 ggccctttca cgctggcttc caccttgaac gagtggccgt gcagcttgcg gtagcgatgg
       61 tctgcgggac cttgctcgat ataatgcgcc gcgtcgaaat gcgcggcctt cgtgatctcg
      121 aagacaggct tcatcaggta cttcttttga caaggcctgg gaggctctag cgcgtcgacc
      181 ctgtcaccaa gtcgagcgcc ttatccgtca gggcaggccg agatatttgt gtgtttggac
      241 gcttaaacgc cattgagggt gggaaaggca ataggcgacg gccaattgcg tgtttgcatc
      301 gcggtcgggg ccgtccatcg gctgaaggta gaagcgctcg aaatccaggt tttcgaagcg
      361 ttcgggcagc gctttctcct gcggatagac gagcttcagt tcctgccccc gggtctgggc
      421 gaccggcgcg tcggccttgg ggctgacgca gatccagtcg atgccgtcgg gcgccgccag
      481 cgtgccattg ctctcgaccg cgatcatgaa gccccgggca tgcagggctg cgatggcggg
      541 cgagtccagc tgcagcagag gctcgccgcc ggtgcacacc accaaccgat caccaggacc
      601 gccggtccag gcggcttcca ccgcgtcggc cagcaactcg gccgtggcga acttcccgcc
      661 accctggccg tccgtgccaa cgaagtcggt gtcgcagaag gcgcagacgg ccttgtggcg
      721 atcctgctcg cggccgctcc acaggttgca gcccgcaaac cggcagaaca ccgccgcctt
      781 gcccgcctgc ccgccttccc cctgcagggt caggaagatt tccttgacgc tataggtcac
      841 ggggccagga ctccgcgccc cgccgcatcc cagtcctcat agcccttctg acgcaggtcg
      901 caggctggac agtcgccgca gccgtgcccc caggggtgca gatcgccgcg ttcgccccgg
      961 tagcaggtgt ggctctcgac cacgatcagg tcgaccaggt cctcgccacc caacgacttg
     1021 gcaagggccc aagtctgcgc cttggtcagc ttcatcagcg gcgtctcgat gcggaagtcg
     1081 cgatccatgc caaggttgag cgcggtctgc atggcgtcca gggtgtcgcg gcgacagtca
     1141 ggatagccag agaagtctgt ctcgcacatg ccgccgacca gggccgcgat accgcgccgg
     1201 tcggccaggg ccgccgcata ggtcaggaac accaggttgc gaccgggcac gaaggtcgag
     1261 ggcagaccgc ggtcggtcat ctcgatggcg cgctcggcgg tcagggccga ctctgcgact
     1321 gcgccaaagc cccgaaggtc gagaacgtgg tcctcgccga gacgctccgc ccactgcgga
     1381 aagcgggcag cgatctgatg gcgcaccgcc acgcgcgcct ccatttcgat gacgtggcgc
     1441 tgaccgtagt cgaagcccac tgtttcgacg cgcccatagc gttccagcgc ccaggcgagg
     1501 cagacgctgc tgtcctggcc gccggaaaat agcaccagag cggcgtcagt catggatggg
     1561 tctccggctc ggccgacacc gggctgcatg aagggcaaaa cacaaggaca tggctccc
//
LOCUS       DAOMEO010000826         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7783_length_1616_cov_2.272902, whole genome shotgun sequence.
ACCESSION   DAOMEO010000826 DAOMEO010000000
VERSION     DAOMEO010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7783_length_1616_cov_2.272902"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLH31_15820"
     CDS             complement(<1..712)
                     /locus_tag="PLH31_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HQR90697.1"
                     /translation="MRLQIAAKPVQAALIDFALQSNLSLGGDLEACRGFAPALFGRMS
                     QKAALDRILTQSGCGFALADPRTILILKVTPQRASRAVVEAATVLGLGEVVVTAQRYP
                     NLPGRTPYAISVISGTTLQREAFTSLADLSAQVAGMTVTNLGPGRDKILLRGLSDGAF
                     TGQTQSMVALYLDDVPITDSAPDPDLRLADIERVEVMRGPQGTLYGGGSLGGVVRTAT
                     RKPDLDRYQANALAGLGHT"
     gene            complement(829..>1616)
                     /locus_tag="PLH31_15825"
     CDS             complement(829..>1616)
                     /locus_tag="PLH31_15825"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQR90698.1"
                     /translation="PARAVADNVSPFQRRAERPSRRWVWAAGATAAAAAALVLAVLPQ
                     LTRPTFTDYSTRRGEIREIALADGSRLTLGSATVVRVHLGRAQRDVTLVDGEARFDVA
                     HLPTRPFVVSVVDRKVRVLGTEFNILSHADRLTVTVRRGVVAVSGGDDPPVRLTKGQQ
                     LIHVQNTTSSQVRVADPNSVLAWTSGKLVYRDTPLPEVVAELNRYVATPIRVDPSAAS
                     VKVSGVLLIDKESAMIRRLELFAPIVSQTSGSEIVLRAKATRP"
BASE COUNT          308 a          515 c          540 g          253 t
ORIGIN      
        1 aagtgtgccc caggccggcc aaagcgttgg cctgatagcg atccagatcc ggctttcgtg
       61 tagcggttcg cacgacgccg cccagggacc cgccgccata gagcgtgcct tgcggaccgc
      121 gcatgacctc gacgcgctcg atatccgcca gccgcagatc ggggtcgggc gcgctgtcgg
      181 tgatcggaac gtcgtcgaga tagagcgcga ccatcgattg ggtctggccg gtgaaggccc
      241 catccgagag gccgcgcagc aggatcttgt cacgcccagg tccgagattg gtgaccgtca
      301 tgccggcgac ctgcgcactg agatcggcca gactggtgaa cgcttcgcgt tgcaaggtgg
      361 tcccggagat gaccgagatc gcatagggcg tgcggccggg taggttgggg tagcgctggg
      421 cggtcacgac cacctcgccg agaccaagaa cggtcgcggc ctcgacgacg gcgcgcgacg
      481 cgcgctgcgg cgtcacctta aggatcagga tcgtgcgagg gtcagccagg gcgaagccgc
      541 agccgctttg agtgagaatt cggtcgagcg ctgctttttg cgacattcgg ccgaacaagg
      601 ccggcgcaaa ccctcgacag gcctccagat cgccgccgag cgaaagattt gattgcaggg
      661 cgaagtcaat cagtgccgct tggacgggct tggcggcgat ctgcaggcgc acgtacggac
      721 cctgctcccg cgccggagcg gcatgcgcag cgccaatcgc caggccggct ataagtgccg
      781 ctagccacac gcggtccaac cgtcgagcca gccccttctt cgaggtcgtc agggtcgggt
      841 cgctttcgcc ctgagtacga tctcgctgcc agacgtctgg ctaacgatcg gggcgaacag
      901 ttccaagcga cggatcatcg cagattcctt atcgatcaaa agcacgccgg agactttcac
      961 agaagccgcg ctgggatcaa cccggatcgg tgtggcgaca tagcggttca gctcggcgac
     1021 cacctcgggt aagggggtat cgcgatagac cagcttgcca gatgtccagg ccaacaccga
     1081 atttgggtcg gccacgcgga cctgcgagct tgtcgtattc tggacgtgga taagttgctg
     1141 gcccttcgtc agtcgtactg gaggatcatc cccgccggac accgcgacca cgccgcgacg
     1201 caccgtgacc gtcaggcgat cggcatggct gagaatattg aactcggtcc ccaacacccg
     1261 caccttccgg tcaacgacgg agacaacgaa gggccgcgtt ggcaggtgcg caacatcgaa
     1321 cctcgcctcg ccatccacca gggtgacgtc acgctgggcg cgcccgagat ggacacgcac
     1381 cacggtggca ctgcccaagg taagcctcga accatccgct aaggcaatct cccggatctc
     1441 gccccgccgg gtggaataat cggtgaaggt tgggcgagtg agttggggca agacggccag
     1501 gacgagcgct gcagcagcag cggcggtagc gcccgccgcc cacacccagc ggcgcgaggg
     1561 tcgctcggcg cggcgttgga acggcgagac attgtcggcc acagcccgcg caggag
//
LOCUS       DAOMEO010000827         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7785_length_1616_cov_2.075593, whole genome shotgun sequence.
ACCESSION   DAOMEO010000827 DAOMEO010000000
VERSION     DAOMEO010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_1616_cov_2.075593"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLH31_15830"
     CDS             <1..409
                     /locus_tag="PLH31_15830"
                     /inference="COORDINATES: protein motif:HMM:NF026799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Brp/Blh family beta-carotene 15,15'-dioxygenase"
                     /protein_id="HQR90699.1"
                     /translation="IGAVAPVTLLVTGVGLVEAWRGGSQAWATAMGLCLIVLFVAPPV
                     IGFAVFFVFLHAPLHLDRARTVLSDMPQSRWLATGALLSGAALIGWLALRLLAPVRID
                     ETLTSQAFQLLAAVAVPHLVLSHWLEQRQDPDV"
     gene            complement(424..939)
                     /locus_tag="PLH31_15835"
     CDS             complement(424..939)
                     /locus_tag="PLH31_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR90700.1"
                     /translation="MKIKDRPEFKSKASVLAFGPDVLVAEAVSVMSERNYGAAVVVSP
                     DRKPLGIVTERDFMRRLLNKRLNPDETTLSQIMTTDLKMAKAEDDLLDWLRIMSNERF
                     RHLPVVDDQGVIISMMSQGDFVSYTWPELLGRLREQAKATFDVSPSLVMIIACIAVFA
                     AWAVTIFTQFR"
     gene            complement(1158..1505)
                     /locus_tag="PLH31_15840"
     CDS             complement(1158..1505)
                     /locus_tag="PLH31_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90701.1"
                     /translation="MTAAIEDLVLGPRAISFCRVSRAGAAAANDDEHEGGSLLMTLQS
                     VEAGDVLGWADIELDGQTTRLETRGADELEVAYELIQLSGAWLIDAAKARGLMLFAWG
                     QTPVVEPMDVFVL"
BASE COUNT          269 a          548 c          518 g          281 t
ORIGIN      
        1 catcggcgcg gtcgctccgg tgaccctgct ggtgaccggc gttggcctcg tcgaggcctg
       61 gcgtgggggc agccaggcct gggccacggc catggggctg tgcctgatcg tgctgtttgt
      121 cgccccgccg gtcattggct ttgcggtgtt tttcgtcttc ctgcatgcgc cgctgcacct
      181 ggaccgggcg cggacggtgc tgagcgacat gccgcaatca cgctggctgg cgacgggggc
      241 gctgctgtcc ggcgcggcgc tcatcggctg gctggccctg aggctgctgg cgcccgtgcg
      301 gatcgatgag accctgacct cgcaggcgtt ccagctgctg gcggccgtcg ccgtgccgca
      361 tctggtcctg tcgcactggc tggagcagcg acaggaccct gacgtttaga gacggggcgc
      421 cctctagcgg aactgggtga agatcgtcac ggcccaggcc gcgaaaacgg cgatacaggc
      481 aataatcatg accagcgagg gactgacgtc aaaggtcgcc ttggcctgtt cccgcagccg
      541 ccccaacagc tctggccacg tgtagctgac aaagtcgccc tggctcatca tgctgatgat
      601 cacgccctga tcatcaacca ccggaaggtg gcgaaatcgc tcgttcgaca tgatgcgcag
      661 ccagtcgaga agatcgtcct cggccttggc catcttcagg tcggtcgtca tgatctggga
      721 cagtgtcgtc tcgtcggggt tcagccgctt gttgagaaga cggcgcatga agtcgcgctc
      781 cgtgacgatc ccgagaggct tgcggtcagg gctcacgacg acggccgccc cgtagttccg
      841 ttcgctcatc accgaaacgg cctcagccac gagcacgtcg ggaccaaagg ccagtacaga
      901 cgccttgctc ttgaactcag gcctgtcctt gatcttcatg gaaagctccc atctggctgc
      961 gcaggtccga aggggtcagg ggcctgcatg gataggcgcg atcgggtttc acccaacgcc
     1021 tccagcagtt cccagaacct ggacgcacct caccctgtga gcggaacaga cggagcacca
     1081 gctgccccaa agtctgtata tcgcgcggca ggaatctgtg ggtgcgcgcc tctcccgcaa
     1141 ggagagagac ccgcccgtca cagcacgaac acgtccatcg gctcgacaac cggcgtctgc
     1201 ccccaggcga acagcatcag ccccctcgcc ttcgccgcgt cgatcagcca ggcgcccgac
     1261 agctggatca gctcataggc gacctcaagc tcatcggcgc cgcgcgtctc gaggcgggtg
     1321 gtctggccgt cgagttcgat atcggcccag ccgagcacat cgccagcctc aaccgactgc
     1381 agggtcatca gcagtgagcc cccttcgtgc tcatcgtcat tggcggcggc ggcgcccgcg
     1441 cgcgagactc ggcaaaagga gatcgcgcgc ggtcccagca ccagatcctc gatcgccgct
     1501 gtcatgggcg cgccgcgaac agttggtgcc atgtggtcac ccgcgtctcg ctgcccggca
     1561 gcagggtcag gcccgcctcc tgcgccgtgc ggatcagcca gtcgccggcg aaccac
//
LOCUS       DAOMEO010000828         1614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7797_length_1614_cov_2.035279, whole genome shotgun sequence.
ACCESSION   DAOMEO010000828 DAOMEO010000000
VERSION     DAOMEO010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7797_length_1614_cov_2.035279"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLH31_15845"
     CDS             complement(<1..284)
                     /locus_tag="PLH31_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HQR90702.1"
                     /translation="MGEYGSDRGEREGQLRADRQAEVAAAGQALAAGGLVLLPTETVY
                     GLGADAANPTAVAAIFEAKGRPRFNPLIAHVADVETAARIAILDDRALALA"
     gene            complement(215..1498)
                     /locus_tag="PLH31_15850"
     CDS             complement(215..1498)
                     /locus_tag="PLH31_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR90703.1"
                     /translation="MAATRTRYTTVAIVIHWLIAAAIIFQIILGWRAGDGPKGQTTFA
                     LYQLHKSIGITILLLSLFRLAWKLTHKSPPPPVGQPNWERIASHVVHWGFYVIMIGLP
                     LTGWIIVSASRTNIPTLLYGVVPWPHLPLLPDLTAGAKAVWHDIGEIGHGMLVKTTYL
                     LLLLHLGAVAKHQILDKDAVFSHMAPGARPGWKEPRAWLAAAGLAAVIAAGYLYMPAV
                     KPAVAAPVVAEEPMTPEPAAASATPADAPVSTEAAPAPMESPLKDPVAWTVSKGGKLG
                     FTASWSGEALEGSFTRWSADILFSPEALDRSKLTVSIDTASAQTGDAQRDESLPSGDF
                     FDTAAHPKATFTATKFRKTGEGRYVADGTLSLRGVSKPLSLPFSLKIDGDTATARGVT
                     TLDRTAFGVGQGEWASTDQIAANVKVSFALTARRK"
BASE COUNT          253 a          540 c          561 g          260 t
ORIGIN      
        1 gccagggcaa gcgcccggtc gtcgaggatg gcgatccgcg cggcggtctc gacatcggcc
       61 acatgggcga tcagcgggtt gaagcgcgga cggcccttgg cctcgaagat cgccgccacg
      121 gccgtggggt tggcggcgtc cgcgcccagg ccatagaccg tctcggtcgg cagcagcacc
      181 aggccgccgg ccgcaagagc ctgcccggcg gccgctactt ccgcctggcg gtcagcgcga
      241 agctgacctt cacgttcgcc gcgatctgat ccgtactcgc ccattcgccc tgtccgaccc
      301 cgaacgccgt ccggtcgagg gtggttacac cgcgcgccgt ggccgtgtcg ccgtcgatct
      361 tcagcgagaa cggcaggctg aggggcttgc tgaccccgcg tagcgacaac gttccgtcgg
      421 cgacatagcg accctcgccg gtcttgcgga acttggtcgc cgtgaaggtc gccttgggat
      481 gcgcggccgt gtcgaagaaa tcgccgctgg gcaggctctc gtcgcgctgg gcgtcgccgg
      541 tctgggccga ggccgtgtcg atgctgacgg tcagcttcga gcggtccagc gcctcgggcg
      601 agaacaggat gtcggcgctc cagcgagtga agctgccttc cagcgcctcg cccgaccacg
      661 aagcggtgaa acccagcttg ccgcccttcg acaccgtcca ggccacggga tccttcaggg
      721 gcgactccat cggcgccggg gcggcctcgg tcgagaccgg ggcgtcggcc ggcgtcgcgg
      781 acgcagcggc tggctcgggc gtcattggct cctcggccac caccggcgcg gcgacggcgg
      841 gcttgacggc gggcatgtag agatagccgg cggcgatcac cgccgcgaga ccggcggcgg
      901 ccagccaggc gcggggctct ttccagcccg gacgggcgcc gggcgccatg tggctgaaca
      961 ccgcgtcctt gtcgagaatc tggtgcttgg ccaccgcgcc caggtgcagc agcagcagca
     1021 gataggtggt cttgaccagc ataccgtggc cgatctcgcc gatgtcatgc cagaccgcct
     1081 tggcgccggc cgtcaggtcg ggcagcagcg gcaagtgagg ccacggaacc acgccgtaca
     1141 gcagggtcgg gatgttggtg cggctggccg agacgatgat ccagcccgtc agcggcaggc
     1201 cgatcatgat cacatagaag ccccagtgca cgacgtgcga ggcgatgcgt tcccagttgg
     1261 gctggcccac cggcggcggc ggcgatttat gcgtcagctt ccaggccagc cggaacaggc
     1321 tgagcagcag gatggtgatc ccgatcgact tgtgcagctg gtagagggcg aaggtcgtct
     1381 gacccttggg accgtccccc gccctccagc cgagaatgat ctggaagatg atggccgcag
     1441 cgatcagcca gtgaatgacg atcgccaccg tcgtgtagcg cgtgcgtgtc gcggccatca
     1501 ggctctccag atctctttta agcgtcttac ttcttgtcga atccgacttc gatgttcagc
     1561 gtcacgtcat cgccgaccaa cggcacatat ttggacacgc cgtattccga gcgc
//
LOCUS       DAOMEO010000829         1611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7819_length_1611_cov_2.597044, whole genome shotgun sequence.
ACCESSION   DAOMEO010000829 DAOMEO010000000
VERSION     DAOMEO010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7819_length_1611_cov_2.597044"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLH31_15855"
     CDS             complement(<1..252)
                     /locus_tag="PLH31_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HQR90704.1"
                     /translation="MTRTILFDSPFLLGFEHTRDLIERAAKAASESYPPYNVEQDDEG
                     GVRITLAVAGFAPEQLHVTVEGGQLVVAGKREAEARPETD"
     gene            complement(497..572)
                     /locus_tag="PLH31_15860"
     tRNA            complement(497..572)
                     /locus_tag="PLH31_15860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(537..539),aa:Ala,seq:ggc)
     gene            complement(726..1193)
                     /locus_tag="PLH31_15865"
     CDS             complement(726..1193)
                     /locus_tag="PLH31_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HQR90705.1"
                     /translation="MANPELGAKQICPNCQSKFYDLGRRPALCPKCGESFDPEEALKS
                     RRVRARAVTPDYDAEDEKPVVAPKEEDGFEDEVDETPEIDKVEPDVIETDDEDADPGA
                     PTPAGGDDLGVDFAEDEDLAEDEADDVPFLEDEDEDDLDDEIEGLPGAGDDDI"
     gene            1309..1563
                     /locus_tag="PLH31_15870"
                     /pseudo
     CDS             1309..1563
                     /locus_tag="PLH31_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
BASE COUNT          272 a          512 c          501 g          326 t
ORIGIN      
        1 gtcggtttcg ggcctggctt cggcctcacg tttgccggcc accaccaact gtccgccctc
       61 gacggtcacg tgcagctgct cgggcgcgaa gccggccacg gccagggtga tgcgcacccc
      121 gccctcatcg tcctgttcga cattataagg cggatagctt tcggacgcag ccttggcggc
      181 gcgctctatc aggtctcgcg tatgttcgaa gcccagcagg aaggggctgt cgaacaggat
      241 cgttcgggtc atcgcgaagt ccttcgctta agcgacttcg tccgccacct cgccctgatg
      301 tcagcagcgg cgcggcgata ccctatggag gtggaggttg ccgcccggcg tttcaaggcc
      361 ggcccggatc agagccactc cacaaagccg catttggcca tccacaacag gctcatcatg
      421 ggccatcgaa aggtggcgat ccatcggtga gggcccgaca tggaaacggc cgctcccttt
      481 agggaagcgg ccgttttggt ggagcttagc ggagtcgaac cgctgacctc ttgcatgcca
      541 tgcaagcgct ctaccagctg agctaaggcc ccgaggtcct tggtttccca agagaaccct
      601 ggtgtttctc gccgcttccg atcaggtcgg ccccggcgag gcgcggaaac tatgccggga
      661 ggatttttgg atcaaccctc ccggcgaact tttttcgaag tatttttcga aggctgtttc
      721 aggccctaga tgtcgtcgtc gccagcgccg ggcagacctt cgatttcgtc gtcgagatcg
      781 tcttcgtcct cgtcttcgag gaagggcacg tcgtcggctt cgtcttcagc cagatcctcg
      841 tcctcggcga aatcgacgcc caggtcatcg ccgcccgcag gggtgggcgc gcccggatca
      901 gcgtcttcgt cgtcggtttc gatgacatcg ggctcgacct tgtcgatttc cggggtctca
      961 tcgacctcgt cctcgaagcc gtcctcctcc ttgggagcaa cgacgggctt ttcgtcctcg
     1021 gcgtcatagt ccggcgtgac ggcgcgggcg cggacgcggc gcgacttcag cgcctcttcc
     1081 gggtcaaacg actcgccaca cttggggcac agagccgggc gacggcccag gtcatagaat
     1141 ttcgattggc agttggggca aatttgtttt gcgcccaatt cgggattggc caagaggcga
     1201 acctttgatc cagatgggaa aagcggccgg tcacttgcca tgaccaaggc ccgctgtcaa
     1261 aagcaatccc ctcgcgaacc ccaccataac cagggagccg gttacggcat gacggcggct
     1321 ggactgaaaa gcgggcccgg cggcgccctt cgcgggaccg ttcgcgctcc cggcgacaag
     1381 tcgatttcgc accgttccat gatcctcggc gccctggcca gcggcacgac cacggtcgag
     1441 ggcctgctcg agggcgacga tgttctggcc accgcgcggg ccatgcgggc ctttggcgcg
     1501 cgggtcgagc aggagggtga ggggcgctgg cgcattgagg gtcacggcgg cttcaccgag
     1561 cccaaccatt attggcatta taagttccca aaatatttcc tttgattgtc c
//
LOCUS       DAOMEO010000830         1611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7824_length_1611_cov_1.847686, whole genome shotgun sequence.
ACCESSION   DAOMEO010000830 DAOMEO010000000
VERSION     DAOMEO010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_1611_cov_1.847686"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLH31_15875"
     CDS             complement(<1..261)
                     /locus_tag="PLH31_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR90706.1"
                     /translation="MDHRRRILIVGGGAAGWLTAAYLARSLRIAEQTHLEITLLESPD
                     VGIIGVGEATFPTIRATLQFLGIDEGRFIRETSATFKQGIRFN"
     gene            complement(309..>1330)
                     /locus_tag="PLH31_15880"
     CDS             complement(309..>1330)
                     /locus_tag="PLH31_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR90707.1"
                     /translation="SLVAGVIPPPSEALKRQALEKAGSLYASRGWTGLGNMSVEAPDL
                     ALMTALAAEKKFHIRVDNYMDPSGAAEVLTKGPFQDASGLIRTQGIKLYMDGALGSRG
                     AALLEPYSDADGAGLLLTERDRGLALMKSAKAVKAQVAMHAIGDRGNRMVLDWFGETL
                     GSDTTARWRIEHAQIVADTDLPRFNAMGVIASMQPSHAIGDLYFAPARLGKARLHEGY
                     RWNGMLRSGAVVAAGSDAPVEVGDPRIEFYAAVHRHSLTGFANEDWRLEEAVSRAQAL
                     KMLTLAPAYAAFAEKDRGTLEAGKKADVTVFSKDLMTVAPAEILTAETVLTVVDGVVA
                     FERRP"
     assembly_gap    1331..1340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1341..>1611)
                     /locus_tag="PLH31_15885"
     CDS             complement(<1341..>1611)
                     /locus_tag="PLH31_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR90708.1"
                     /translation="AAKDVRDFDLKGAAAFPGFVDAHAHLTGIGLRELTLNLDQVGSV
                     EALIKAVAAHAAANPDGPIYGRGWIETHWPEKRFPSRADLDRAAPG"
BASE COUNT          237 a          552 c          548 g          264 t
ORIGIN      
        1 gttgaagcgg attccctgct tgaacgtggc cgaggtctcg cggatgaacc ggccctcgtc
       61 gatgcccagg aactgcagcg tcgcgcggat ggtcgggaaa gtagcctcgc ccaccccgat
      121 gatgcccaca tcgggagatt ccagcagggt gatctcgaga tgggtctgct cggcgatccg
      181 cagcgacctg gccagatagg cggcggtcag ccaacccgcc gcgccgcccc ccacgatcag
      241 gattctgcgt ctgtgatcca tgctctctgc atagcgcctt atgggctgta ttggcggcta
      301 aatcgcgctc acggcctccg ctcgaacgcc accaccccgt cgaccacggt cagcaccgtt
      361 tcggcggtga ggatctcggc cggggcgacg gtcatcaggt ccttggagaa cacggtcacg
      421 tcggccttct tgccggcctc cagcgtgccg cgatcctttt cggcgaaggc ggcataggcg
      481 ggcgccaggg tgagcatctt cagggcctgg gcgcggctga cggcctcctc caggcgccag
      541 tcctcgttgg cgaagccggt caggctgtgg cggtgcacgg cggcgtagaa ctcgatgcgc
      601 ggatcgccca cctcgaccgg ggcgtccgag ccggcggcca cgaccgcgcc gctcctgagc
      661 attccgttcc agcgatagcc ctcatgcagg cgggccttgc ccaggcgggc gggggcgaaa
      721 tagaggtcgc cgatggcgtg gctgggctgc atcgaggcga tgacgcccat ggcgttgaag
      781 cgcggcaggt ccgtgtcggc gacgatctgg gcgtgctcga tccgccaacg ggcggtggtg
      841 tcactcccaa gcgtttcgcc aaaccagtcc agcaccatgc gattgccgcg atccccgatg
      901 gcgtgcatgg ccacctgggc cttcacggcc ttcgccgact tcatcagcgc cagccccctg
      961 tcgcgctcgg tcagcaacag gccggcgccg tcagcgtcgc tgtaaggctc cagaagcgcc
     1021 gcgccgcgcg agcccagggc gccgtccata tagagcttga tgccctgcgt gcggatcagg
     1081 ccgctggcgt cctggaacgg gcccttggtc agcacctctg cagcgccgct ggggtccatg
     1141 tagttgtcga cccggatgtg gaacttcttc tcggcggcca gcgcggtcat cagggccagg
     1201 tccggcgcct cgacgctcat gtttccaagc cccgtccagc cgcgcgaggc gtagagggag
     1261 ccggccttct ccagagcctg acgcttcagg gcctccgacg gcggcggaat cacgccggcc
     1321 accagcgact nnnnnnnnnn cccggggcgg cgcggtccag atcagcgcgg ctcggaaagc
     1381 gcttctctgg ccagtgggtc tcgatccagc cccggccata gatcggcccg tccgggttgg
     1441 cggcggcatg ggcggccacc gccttgatca gcgcctcgac cgagccgacc tggtccaggt
     1501 tcagggtcag ttcccgcagg ccgatgccgg tcagatgggc gtgagcgtcg acaaagcccg
     1561 ggaaggcggc ggcgcctttc aggtcgaagt cgcggacgtc cttggccgcc c
//
LOCUS       DAOMEO010000831         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7832_length_1610_cov_2.104180, whole genome shotgun sequence.
ACCESSION   DAOMEO010000831 DAOMEO010000000
VERSION     DAOMEO010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7832_length_1610_cov_2.104180"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLH31_15890"
     CDS             <1..733
                     /locus_tag="PLH31_15890"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQR90709.1"
                     /translation="LGYAEADPTMDVGGFDAGHKITILAALAFGCAPNFAAAEIEGIE
                     AVELLDIKLAKYLGYRIKLVASAARTEDGVAVKVHPSLAPLEHPLAQAGGALNALFIE
                     GRRVGRIFVQGPGAGAGPTAAAVAADIADVMTGAVRPVFQAPSGNLKPFIAVDPSRTV
                     GKAYLRIMVRDEPGAIAAISETLAECGVSIDSFLQKPVEGAGGVPIVLVTHATPESNL
                     LDAITRIEKLRAVLERPRLLRVARI"
     assembly_gap    824..833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <834..>1610
                     /gene="glpX"
                     /locus_tag="PLH31_15895"
     CDS             <834..>1610
                     /gene="glpX"
                     /locus_tag="PLH31_15895"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HQR90710.1"
                     /translation="AVRVTEAAAIASWTMVGRGDEIAADQAAVDAMRNALNELAINGE
                     IVIGEGERDEAPMLYIGEKVGTGKGPAIDIALDPLEGTTLAAKAMPNALTVMAWAPKG
                     TLLNAPDTYMDKIACGPGLPAGVIDLDKSPAENVQAIAAAKGVPASQITVCVLDRPRH
                     AEIIASVRSVGARVYLITDGDVAGVINTTDPSTGIDLYVGSGGAPEGVLACAALKCVG
                     GQFQGRLLFRNADERARAAKWGITELDKKYDLHEIVRADAI"
BASE COUNT          265 a          530 c          528 g          277 t
ORIGIN      
        1 cctgggctat gccgaggccg atccgaccat ggacgtcgga ggcttcgacg ccggccacaa
       61 gatcacgatc ctggcggccc tggccttcgg ctgcgcgccc aatttcgcgg ccgccgagat
      121 cgagggtatc gaggccgtcg agctgctgga tatcaagctg gccaagtatc ttggctaccg
      181 gatcaagctg gtcgcctcgg ccgctcgcac cgaggacggc gtggcggtca aggtgcatcc
      241 gtcgctggct ccgctggagc atccgctggc ccaggctggc ggcgccctca acgccctgtt
      301 catcgaaggc cgccgcgtcg gccggatttt tgtgcagggg ccgggggctg gcgcggggcc
      361 gaccgccgcc gccgtggcgg ctgatatcgc cgatgtgatg accggcgccg tgcgcccggt
      421 tttccaggcg ccctcaggca acctcaagcc tttcatcgct gtcgatccca gtcgcacggt
      481 gggcaaggcc tatctgagga tcatggtgcg cgacgaaccc ggcgccatcg ccgccatctc
      541 tgagaccctg gcggagtgtg gtgtctcgat cgatagcttc ctgcaaaagc ctgtcgaagg
      601 cgcgggcggg gtgccgattg tgctcgttac ccatgcgacg ccggaatcca atctcctgga
      661 tgcgattaca cgcatcgaaa aacttcgcgc cgtgctagag cgtccccgcc ttttgcgcgt
      721 tgcgcgcatc tgagaaccgc gaaaagcggc cgagatttag ccttacaagg ctgggagacg
      781 ttgatgagtt cgaagaccct ggaccgctcg ctggtcctgg acgnnnnnnn nnngcggtcc
      841 gcgttaccga agccgccgcc attgcgtcct ggaccatggt cggtcgcggc gacgagatcg
      901 ccgccgatca ggccgccgtc gacgccatgc gcaacgcgct caatgaactg gccatcaatg
      961 gcgagattgt catcggcgaa ggcgagcgcg acgaagctcc catgctttat atcggcgaga
     1021 aggtcggcac gggcaaaggc ccggccatcg acatcgccct ggatccgctc gagggcacga
     1081 ccctggccgc caaggccatg cccaacgccc tgaccgtgat ggcctgggcg cccaagggta
     1141 cgctgctgaa cgcccccgac acctatatgg acaagatcgc ctgcgggcct ggcctgccgg
     1201 ccggcgtgat cgatctggac aagtcgcctg ccgagaacgt tcaggccatc gcggccgcaa
     1261 agggcgtgcc ggcctcgcag atcactgtct gtgtgctgga tcgcccgcgc catgccgaaa
     1321 tcatcgccag cgtccgctcg gttggcgcgc gggtctatct gatcacggac ggtgatgtcg
     1381 cgggcgtgat caacacgacc gatccttcga caggcatcga tctctatgtc ggctccggcg
     1441 gcgcgcctga gggcgtgctg gcctgcgcgg cgctcaagtg cgtcggtggg cagttccagg
     1501 gtcgcctgtt gtttcgcaat gccgacgagc gcgcccgggc cgccaaatgg ggcatcaccg
     1561 agctcgacaa gaagtatgac ctgcacgaga tcgtccgcgc cgacgccatc
//
LOCUS       DAOMEO010000832         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7846_length_1607_cov_2.704253, whole genome shotgun sequence.
ACCESSION   DAOMEO010000832 DAOMEO010000000
VERSION     DAOMEO010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7846_length_1607_cov_2.704253"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..>505)
                     /locus_tag="PLH31_15900"
     CDS             complement(14..>505)
                     /locus_tag="PLH31_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287649.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQR90711.1"
                     /translation="KEAGADPKAARARAMVAAFREADGGPPRIMVAKMGQDGHDRGQK
                     VIASAFSDLGFQVEIGPLFQTPAEAAEEAIRAGVHVVGASSLAAGHLTLVPELKAELA
                     RLGREDMVVVVGGVIPPQDFQALLDAGAASIFPPGTVIAEAAIEVLEKLNVQLGYTQG
                     AAA"
     assembly_gap    506..515
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<516..>1607)
                     /locus_tag="PLH31_15905"
     CDS             complement(<516..>1607)
                     /locus_tag="PLH31_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQR90712.1"
                     /translation="QNDILKEFMVRNTYIYPPAPSMRIISDIFSYTSANMPKFNSISI
                     SGYHMQEAGATADLELAYTLADGIEYARAGVAAGMSIDVFAPRLSFFWAIGMNYFMEV
                     AKMRAARLLWARLMKREFDPKDDRSLSLRTHSQTSGWSLAAQDVFNNVSRTCVEAMAA
                     VNGQTQSLHTNALDEALALPTDFSARIARNTQLFLQMESGTTRVADPWGGSYYVERLT
                     HDLAQKALAHIEEVEAAGGMAKAIERGIPKLRIEEAAAKTQARIDANRQSVVGVNRYK
                     PEVEDVIPMLKVDNSSVRAQQLEKLARLKAERDPAATAAALKALEDGARGHGNLLALA
                     VDAARAKATVGEISLAMENVFGRHRAVIQT"
BASE COUNT          222 a          522 c          574 g          279 t
ORIGIN      
        1 gggcgccccg cgactacgcc gccgccccct gcgtgtagcc cagctgcacg ttcagctttt
       61 cgagcacctc gatggccgcc tcggcgatca ccgtgccggg ggggaagatg ctggccgcgc
      121 cagcgtcgag cagggcctgg aagtcctggg gcgggatcac gccgccgacc accacgacca
      181 tgtcctcgcg cccgagtcgg gccagttccg ccttcagctc cggcacaagg gtcaggtggc
      241 ctgccgccag ggacgaggcc ccgaccacgt gaacgccggc ccggatcgct tcctcggcgg
      301 cttcggcggg ggtctggaac agcgggccga tctcgacttg gaagccgagg tcggagaagg
      361 cgctggcgat caccttctgg ccgcggtcgt ggccgtcctg gcccatcttg gcgaccatga
      421 tgcggggcgg gccgccgtca gcctcgcgga aggcggcgac catggcgcgg gcgcgggcgg
      481 ccttgggatc ggccccggct tccttnnnnn nnnnngtctg gatcacggcg cggtggcgac
      541 caaagacgtt ctccatcgcc aggctgatct cgcccaccgt ggccttggcg cgggcggcgt
      601 caacggccag ggccagcagg ttgccatggc cgcgcgcgcc gtcctccagc gccttgagcg
      661 ccgccgccgt ggcggccggg tcgcgctcgg ccttcaggcg ggccagcttt tccagctgct
      721 gggcgcggac cgagctgttg tccaccttca gcattgggat cacgtcctcg acctcgggct
      781 tgtagcgatt gacgcccacc acgctctggc gattggcgtc gatgcgggcc tgggtcttgg
      841 cggcggcctc ctcgatgcgc agcttgggaa tgccgcgctc gatggccttg gccatgccgc
      901 cggccgcttc cacttcctca atgtgggcca gggccttctg ggccaggtcg tgggtcaggc
      961 gctcgacata gtagctgccg ccccacgggt cggcgacgcg ggtcgtgccg ctctccatct
     1021 gcaggaacag ctgggtgttg cgggcgatgc gggccgagaa gtcggtgggc agggccaggg
     1081 cttcgtccag ggcgttggtg tgcagactct gggtctggcc gttcacggcc gccatggcct
     1141 cgacgcaggt gcgcgagacg ttgttgaaca cgtcctgggc cgccagcgac cagcccgagg
     1201 tctggctgtg ggtgcgcagg gagaggctgc gatcgtcctt gggatcgaac tcgcgcttca
     1261 tcaggcgggc ccacagcaga cgggccgcgc gcattttcgc gacctccatg aagtagttca
     1321 tgccgatcgc ccagaagaag ctgaggcgcg gtgcgaacac gtcgatgctc atgccggccg
     1381 cgacgccggc ccgggcatat tcgatgccgt cggccagggt gtaggccagc tccaggtcgg
     1441 cggtcgcgcc ggcctcctgc atgtgatagc cgctgatcga gatcgaattg aacttcggca
     1501 tgttggccga agtgtatgaa aagatgtcgg aaatgatccg catcgagggc gcgggcggat
     1561 agatataggt gttccgcacc atgaattctt tgaggatgtc gttctgg
//
LOCUS       DAOMEO010000833         1606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7851_length_1606_cov_1.982592, whole genome shotgun sequence.
ACCESSION   DAOMEO010000833 DAOMEO010000000
VERSION     DAOMEO010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7851_length_1606_cov_1.982592"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLH31_15910"
     CDS             <1..592
                     /locus_tag="PLH31_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR90713.1"
                     /translation="AAFLLTITPGLDTALVLRTAATEGSRRAFAAALGIGIGCLVWGV
                     AAACGAGALLTASEVAYTALKWAGAAYLVWIGIRMILKPRAAFEPGAMAPTGGGAMAS
                     LRRGLLTNLLNPKVGVFYISFLPQFVPAGANPATFGVVLAGLHVVMGLVWAGLLIAAT
                     TPLAGLLREASVIRWLDRITGGVFIAFGLRLALERR"
     gene            complement(589..>1606)
                     /locus_tag="PLH31_15915"
     CDS             complement(589..>1606)
                     /locus_tag="PLH31_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQR90714.1"
                     /translation="RVPWIVVWDDHETANDSWQGGAENHDPDKGEGDWAARKAAALKA
                     YYEWMPIREPAAGALPEASWRGFQFGDVATLLMTETRLTARTEAVDYARDLPMVEGKP
                     DVAAFSAKWKDPSRRLLGEAQAQWLAGQVGASVKGGCAWQVLGNQVVMARVAGPNLVQ
                     VMGPEKFEAMLGKLPEYIRGRVRQGVGLSALGLPYNLDAWDGYPADRERLYDLFKSAG
                     ANPIVLSGDSHSFWANELFDDAGAKRVAVEFGATGITSPGYGDILPGVPLGDAFAARN
                     KEVKFADPGAKGFVLLTLEHGKATSEMIAVSTIHDPAYTTRVLKRFVVTPTKGGGVEA
                     LAEG"
BASE COUNT          215 a          577 c          546 g          268 t
ORIGIN      
        1 cgccgccttc ctgctgacca tcacgccggg tctcgacacg gcgctcgttc tgcgaaccgc
       61 cgccaccgaa ggttcgagac gggcgttcgc ggcggcgctg ggcatcggga tcggctgtct
      121 ggtctggggc gtggcggccg cctgcggagc cggcgcactg ctgaccgcgt ccgaggtcgc
      181 ctataccgct ctgaaatggg ccggcgcagc ctatctggtc tggatcggga tcaggatgat
      241 cctcaagccc cgcgccgcct ttgagccggg cgccatggct cccaccggcg gcggcgcgat
      301 ggcctccctg cggcgggggc tgctgaccaa tctgctcaat cccaaggtcg gggtgttcta
      361 tatctcgttc ctgccgcagt tcgtgccggc cggcgccaac ccggccacct tcggcgtggt
      421 gctggcggga ctgcacgtgg tgatgggcct ggtctgggcc gggctgttga tcgccgccac
      481 cacgcctctg gccggcctgc tgcgcgaggc ctcggtgatc cggtggctgg accggatcac
      541 cggcggcgtc ttcatcgcct tcggcctgcg cctggcgctg gagcggcgtt agccctccgc
      601 aagcgcctcg acgccgccgc ccttggtggg cgtgaccaca aagcgcttga gcaccctggt
      661 ggtgtaggcg ggatcatgga tggtcgagac ggcgatcatc tcgctggtgg ccttgccatg
      721 ttccagggtc agcagcacga agcccttggc gcccggatcg gcgaacttga cctccttgtt
      781 gcgcgccgcg aaggcgtcgc ccagcggcac gcctggcagg atgtcgccat agcccggcga
      841 ggtgatcccc gtcgcgccga actcgacggc cacgcgcttg gcgccggcgt catcgaacag
      901 ctcattggcc cagaacgaat ggctgtcgcc cgacagcacg atcggattgg cgccggcgct
      961 cttgaacaga tcgtacagac gctcgcggtc ggcgggatag ccgtcccagg cgtcgagatt
     1021 gtagggcagg cctagggccg acaggcccac gccctgacga acacgcccgc ggatatactc
     1081 gggcagcttg cccagcatgg cctcgaactt ctcgggaccc atcacctgga ccagattggg
     1141 tccggccacc cgggccatga ccacctgatt gcccagcacc tgccaggcgc agccaccctt
     1201 gaccgaggcg ccgacctgcc ccgccagcca ctgggcctgg gcctcgccca gcaaacggcg
     1261 gctggggtcc ttccacttgg ccgagaacgc ggccacgtcg ggcttgccct cgaccatcgg
     1321 caggtccctg gcgtagtcga ccgcctcggt gcgcgcggtc aggcgggtct cggtcatcag
     1381 cagggtggcg acgtcaccga actggaagcc gcgccagctg gcctcgggca aggccccggc
     1441 cgccggctcg cggatcggca tccactcgta ataggccttc agggcggcgg ccttgcgggc
     1501 ggcccagtcg ccttccccct tgtccgggtc gtggttctcg gccccgccct gccagctgtc
     1561 attggcggtc tcgtggtcgt cccagaccac gatccacggc acgcgc
//
LOCUS       DAOMEO010000834         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7864_length_1603_cov_2.625969, whole genome shotgun sequence.
ACCESSION   DAOMEO010000834 DAOMEO010000000
VERSION     DAOMEO010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7864_length_1603_cov_2.625969"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1186)
                     /locus_tag="PLH31_15920"
     CDS             complement(80..1186)
                     /locus_tag="PLH31_15920"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQR90715.1"
                     /translation="MTDTAPPAPSWPLLPGSTIGILGGGQLGRMLALAAARLGFDVVI
                     LDPEENSPAGRVAARQIVAAYDDRWALKRLADACDVVTYEFENVPADTVAELTALGCV
                     VAPGAKALAAAQDRVVEKSFLAEIGVPTVAFAAVDDSETLTAAIAKIGAPSLLKTRRE
                     GYDGKGQAWIQRASEAQASFEKIGRQAAILEAPADFGRELSVIAARSRDGEIACYPLS
                     ENHHEGGVLKRTSAPAKVTTATRDQAETIAIRILTALDYVGVIGVELFELAGGKLLVN
                     EFAPRVHNTGHWTQDGCEVDQFEQHIRAVAGWPLGPTQPLAHVEMTNLLGADVEAWRK
                     LAAEPETRVHLYGKGEARPGRKMGHVNRLRGLKD"
     gene            complement(1192..1602)
                     /gene="purE"
                     /locus_tag="PLH31_15925"
     CDS             complement(1192..1602)
                     /gene="purE"
                     /locus_tag="PLH31_15925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQR90716.1"
                     /translation="MTSLGVAFEARVISAHRTPQRLFDFASGAQAAGFKVIIAGAGGA
                     AHLPGMAAAMTNLPVLGVPVQSKALSGLDSLLSIVQMPGGVPVATLAIGEAGAKNAGL
                     LAAQILALSDPALAERLAALRQAQTDSVAESVED"
BASE COUNT          229 a          520 c          605 g          249 t
ORIGIN      
        1 aacaatatca gcccgccttc ccaggccttg tgcctgggaa ggcaaacttg agttagggcg
       61 atggataaag ggaacccctt tagtccttca gcccccgcag gcggttcaca tggcccatct
      121 tgcggcccgg gcgggcctcg cccttgccgt agagatggac gcgggtctcg ggttcggcgg
      181 ccagcttgcg ccaggcctcg acatcggcgc ccaagaggtt ggtcatttcc acatgggcca
      241 ggggctgggt ggggcccagg ggccagccgg ccacggcgcg gatgtgctgc tcgaactggt
      301 cgacctcgca gccgtcctgg gtccagtggc cggtattgtg gacgcggggg gcgaactcgt
      361 tgaccagcag cttgccgccg gccagctcga acagctccac gccgatcacg cccacataat
      421 ccagggccgt cagtatacgg atggcgatgg tttcggcctg gtcgcgggtg gcggtggtga
      481 ccttggccgg ggccgaggtg cgtttcagca cgccgccctc gtggtggttt tccgatagcg
      541 gatagcaggc gatctcgccg tcgcggctgc gcgcggcgat gaccgacagc tcgcgcccga
      601 aatcggcggg ggcctcgagg atggccgcct ggcgaccgat cttctcgaac gaggcctgcg
      661 cctcgctggc ccgctggatc caggcttggc ccttgccgtc atagccctcg cggcgggttt
      721 tcagcaggga gggggcgccg atcttggcga tggcggcggt cagggtttcg ctgtcgtcga
      781 cggcggcgaa ggccacggtg ggtacgccga tctcggccag gaagctcttt tcgacgacgc
      841 ggtcctgggc ggcggccagg gccttggcgc ccggcgcgac gacgcagccc agggcggtca
      901 gttcggcgac ggtgtcggcc gggacgttct cgaactcata ggtgaccacg tcgcaggcgt
      961 cggccaggcg cttgagggcc cagcggtcgt cataggcggc gacgatctgg cgggcggcga
     1021 cacggccggc ggggctgttt tcctcggggt cgagaatgac cacatcgaac cccaggcgcg
     1081 cggcggccag ggccagcatc cggcccagct gtccgccgcc caaaatgccg atggtcgagc
     1141 cggggaggag gggccaggag ggggcgggag gggccgtgtc tgtcatgccg gtcagtcctc
     1201 gacgctttcg gcgacgctgt cagtctgcgc ctgccgcaag gcggccagac gctcggccag
     1261 ggcggggtcg gacagggcca ggatctgggc ggccagcaag ccggcgttct tggccccggc
     1321 ctcgccgatc gccagggtgg ccacgggcac gccgccgggc atctggacga tggaaagcaa
     1381 ggagtcgagg ccgctcaggg ccttggactg cactggcacg cccaacaccg gcaggttggt
     1441 catcgcggcg gccatgcccg gcaggtgggc cgcgccgccg gccccggcga tgatcacctt
     1501 gaagcccgca gcttgcgcgc cgctggcgaa gtcgaacagg cgctgcggcg tgcggtgggc
     1561 cgagattacc ctggcctcga aggcgacgcc caggctggtc agc
//
LOCUS       DAOMEO010000835         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7867_length_1602_cov_2.448610, whole genome shotgun sequence.
ACCESSION   DAOMEO010000835 DAOMEO010000000
VERSION     DAOMEO010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_1602_cov_2.448610"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLH31_15930"
     CDS             complement(<1..482)
                     /locus_tag="PLH31_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQR90717.1"
                     /translation="MARDPYQELGVSRTATTDEIRKAFRKLAKQFHPDTNPGNKAAED
                     RFKQVSAAFDILGDAVKKKKFDAGEIDADGREIARGFGGAGGSPFGAGGFNRGGFQSR
                     GGGADGPEIDLGDLFGDILGRGARGGGFAGGASGGGFSPRGADVRARLDIDLEEAIRG
                     G"
     gene            complement(565..>1335)
                     /locus_tag="PLH31_15935"
     CDS             complement(565..>1335)
                     /locus_tag="PLH31_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90718.1"
                     /translation="TTPATLGETLSDLPPASPHRLDRANRLTVRLEGATPASRSLAAV
                     LAGDNALAVLTPSGEWEVIGFTSAEAVAEDVWTLSGLLRGQRDGAAAEAVILSGAPVV
                     LLDEALARLEVAPFERGAPLIVRAAPAGGPPSGPAMSEVGMTWRGRALRPLAPAHLRV
                     RVSGGDRLVNWIRRDRLGGDVWEGEVPLSEAVEAWRLRVLNGAVVVREVALAAPAFVH
                     TAAMQVADLAAGSAGVLTVEVAQGSAASGWGVTATRLL"
     assembly_gap    1336..1345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1346..>1602)
                     /locus_tag="PLH31_15940"
     CDS             complement(<1346..>1602)
                     /locus_tag="PLH31_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90719.1"
                     /translation="RVDLDEHPRATLAPVPAGSELDSRIDWTPAPPREPVGPPALLML
                     DLPAISDDARPWVAAAAEPWRPFDIHAGGDLDTLKVRTRLT"
BASE COUNT          238 a          601 c          550 g          203 t
ORIGIN      
        1 ccgccgcgaa tggcctcttc caggtcgatg tcgagcctgg cccgcacatc ggcgccgcgc
       61 ggcgagaacc cgccgccgct ggccccgccg gcaaagccgc cgccccgggc gccgcgcccc
      121 aggatgtcgc caaacaggtc gcccaggtcg atctcgggac catcggcccc gccgccgcgc
      181 gactggaagc cgccgcgatt gaagccgccg gccccgaacg ggctgccgcc cgccccgcca
      241 aagccgcgcg cgatctcgcg accgtcggcg tcgatctcgc cagcgtcgaa cttcttcttc
      301 ttgaccgcgt cgcccaggat gtcgaaggcg gcgctgacct gcttgaagcg gtcctcggcg
      361 gccttgttgc ccgggttggt gtccgggtgg aactgcttgg cgagcttgcg gaacgccttg
      421 cggatttcgt ccgtcgtcgc ggtccgggaa acaccaagtt cctgataggg gtcgcgcgcc
      481 aaggaaggtc ctcgagagtg attggaatac ctgccctcac ttaggccatt cgtggccgca
      541 cacaagcaca ttgttgcatt attatcacag caaccttgtc gcagtgacgc cccaaccgct
      601 cgcggccgag ccctgggcga cctcgacggt cagcacgccg gccgatcccg ccgccagatc
      661 agcaacctgc atggcggcgg tatggacgaa ggctggcgcg gccagcgcga cctcgcgaac
      721 gaccaccgcg ccattcagca cccgcaggcg ccaggcctcg acggcttcgc tgagcggaac
      781 ctcgccctcc cagacatcgc cgcccaggcg gtcgcgacgg atccagttga ccaggcgatc
      841 gccgcccgag acccgtacgc gcagatgcgc cggagccagc ggacgcaggg cccggccgcg
      901 ccaggtcatg ccaacctcgc tcatggccgg tcccgagggc ggaccaccgg ccggcgcggc
      961 ccgcacgatc agcggcgcgc cccgctcgaa gggcgcgacc tccagccggg ccagcgcttc
     1021 atccagtaga accacgggcg cgccggacag gatcaccgcc tcagccgccg cgccatcgcg
     1081 ctggccgcgc agcaggcccg acagggtcca gacatcctcg gcgaccgcct cggccgacgt
     1141 gaagccgatg acctcccatt cgccggatgg cgtcagcacc gccagggcgt tgtcgccggc
     1201 cagcacggcg gccagcgagc ggctggccgg ggtcgccccc tccagccgca cggtcaggcg
     1261 gttggcgcgg tccagccgat gcggcgaggc cggcggcagg tcggagaggg tctcgcccag
     1321 ggtggccggc gtcgtnnnnn nnnnncgtga ggcgggtgcg gaccttcagg gtgtccagat
     1381 cgccgccggc atgaatatcg aacggccgcc agggctcggc ggcggcggcg acccacggcc
     1441 gggcgtcgtc gctgatggcc ggcaggtcga gcatcaacaa cgccggaggg cccacaggtt
     1501 cccgcggcgg cgcgggtgtc cagtcgatgc ggctgtccag ctcggagccc gccgggaccg
     1561 gcgccagggt cgcgcgcgga tgctcgtcca gatccacccg ga
//
LOCUS       DAOMEO010000836         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7879_length_1601_cov_1.919146, whole genome shotgun sequence.
ACCESSION   DAOMEO010000836 DAOMEO010000000
VERSION     DAOMEO010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7879_length_1601_cov_1.919146"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1262
                     /locus_tag="PLH31_15945"
     CDS             <1..>1262
                     /locus_tag="PLH31_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90720.1"
                     /translation="KASWRSVDHEMNFLADKGGSVTGGMPMNTQGRDLRASLAVSVPQ
                     KDGGAIRAEVEAHRGDVDDWWPPVAGSMMMGPQTYWNINNGRRERTGLWAEWEAKPSA
                     AWTTLLGARVEHVETDAGLVQPYAWTGMMNMPDAMAARAFNARERGRADDNVDVTAKA
                     VWRANTTTSIEFGYARKTRSPTLYERYAWGKGAMSSSMTSFAGDANAYVGDPDLSPEV
                     AHNLAATVKYASLDGKRGLTLSAYHSRVDDFIDAVKLADLTKGFVQLRFANVDARLHG
                     FDASGYAQVWETAGLGQGTLKGVLSWVEGENRNSGDNLYHMTPLTLRLALEQTKGRWK
                     NQAEVELVGEKDAVNALRHEPRTSAYALFSLRSSWKGDRVRVNLAAENLLDTAYDLPL
                     GGVSFGDFKAGGSVAAIQPLPGPGRSINL"
     assembly_gap    1263..1272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          300 a          500 c          535 g          256 t
ORIGIN      
        1 aaggcgtcgt ggcgctcggt ggaccacgag atgaacttcc tggccgacaa gggcgggtcc
       61 gtcacgggcg gcatgccgat gaacacccag ggccgggatc tcagggcctc gctggcggtg
      121 agcgtgccgc agaaggatgg cggggccata cgcgccgagg tcgaggccca ccgcggcgac
      181 gtcgacgatt ggtggccgcc cgtcgcgggc agcatgatga tggggccgca aacctactgg
      241 aatatcaaca atggccgccg ggagaggacc gggttgtggg cggaatggga ggccaagccc
      301 tcggcggcgt ggacgacctt gctcggcgcg cgggtcgagc atgtagagac tgacgccggt
      361 ctggttcagc cctatgcctg gaccggtatg atgaacatgc ccgacgccat ggccgcgcgc
      421 gctttcaacg cccgcgagag gggtcgcgcc gacgacaatg tagacgtcac cgccaaggcc
      481 gtctggcggg cgaacaccac gacgagcatc gagttcggct acgcgcgcaa gacccgctcg
      541 cccacactct acgaacgcta cgcctggggc aagggggcga tgtcgagctc aatgacctcc
      601 tttgctggag acgccaacgc ctatgtcggc gatccggatc tgtcgcccga agtcgcgcac
      661 aatctggcgg ccacagtgaa atacgccagc ctggacggga agcgtggcct gacgttgagc
      721 gcctaccatt cccgggtcga tgattttatc gacgcagtga aattggccga cctgacgaag
      781 ggctttgttc aactgcgctt cgccaatgtg gacgcgcgcc tgcacggctt cgacgccagc
      841 ggctacgctc aggtttggga aaccgccggg ctgggccaag ggaccttgaa aggcgtcctg
      901 tcctgggttg agggcgaaaa ccgcaatagc ggtgacaatc tctatcacat gacgccgctg
      961 accctgcgcc tggccctgga acaaacgaag ggccgctgga aaaatcaggc cgaggtggaa
     1021 ctggtcggcg agaaagatgc ggtcaatgcc ctgcgccacg agcccaggac gtccgcctac
     1081 gctctgttca gcctgcgctc cagttggaag ggggatcggg ttcgcgtgaa tctggccgcg
     1141 gagaacctgc tcgacaccgc ctacgatctg ccgctgggcg gggtgtcgtt cggcgacttc
     1201 aaggccggcg gctctgtcgc ggcgatccag ccgctgccag gaccgggtcg gtcgatcaac
     1261 ctnnnnnnnn nngcgggctg gctttgcaca agcaggctcc ggcgtttgcg ccggagcctg
     1321 cgatgaccgt cagtctggtc gaggcgccgc cgcgcgagac tgcgcgatgg ccggcgacat
     1381 tcgtcatccg gccaaactcg ccgcctaact cgcaggtttc gacagcaatt ccacagatcg
     1441 aggcatccgc gccttctatc ggcgccaggc tcgaaccggc tggggagttg acctcgctag
     1501 agccgccatc tcctcagcct gtggcagggg ggaccataac ccccttgcca acaacgatca
     1561 gcaaacctga agaagacttg ggctccgact atggacgacg c
//
LOCUS       DAOMEO010000837         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7882_length_1600_cov_2.620712, whole genome shotgun sequence.
ACCESSION   DAOMEO010000837 DAOMEO010000000
VERSION     DAOMEO010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_1600_cov_2.620712"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLH31_15950"
     CDS             <1..667
                     /locus_tag="PLH31_15950"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQR90721.1"
                     /translation="TDPKGKIWNGYSPLGLRWIAEANRLGIVIDVSHASDDVVDQSVA
                     LSKVPIIASHSGPKAIYDHARNMDDARLKKIADAGGAICINSIYLTNTTPSPERKAAL
                     EALGRGPDLKTATPEVVKAYADKRAAIDKAYPPVRGDFDLFMKSMLHVLKVTGPKGVC
                     VGADWDGGGGMDGFEDITDLPKVTARLKAEGYSDADIEGIWSGNVLRILAAAEAYAKS
                     LAK"
     gene            complement(711..1331)
                     /gene="msrQ"
                     /locus_tag="PLH31_15955"
     CDS             complement(711..1331)
                     /gene="msrQ"
                     /locus_tag="PLH31_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HQR90722.1"
                     /translation="MIGAKPARKRPSKLLDNLVYGLVWLACLAPLVWLAWRGFTGDLG
                     ANPIETLIRQLGVWGLRLLLVGLAITPLARILRMPRLVRFRRTVGLFAFSYIVLHLFS
                     YVGVDLFFDLGQLWKDILKRPFITLGMGAFLLLIPLAVTSTNGWVIRMGRATWQRLHW
                     LIYLIVPLGVAHYYLLVKADHRPPLIYAAVFAVLMSWRIWDRVKRT"
     gene            complement(1331..>1600)
                     /locus_tag="PLH31_15960"
     CDS             complement(1331..>1600)
                     /locus_tag="PLH31_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mononuclear molybdenum enzyme YedY"
                     /protein_id="HQR90723.1"
                     /translation="VPWKYGFKGIKSIVRITLTEKQPRTAWNVSAPREYGFYSNVNPA
                     VDHPRWSQASERRIGEFRRRETLPFNGYGQYVADLYRGMDLKKNF"
BASE COUNT          323 a          518 c          519 g          240 t
ORIGIN      
        1 gaccgatccc aagggcaaga tctggaacgg ctattcgccg ctgggcctgc gctggatcgc
       61 cgaagccaat cgcctgggca ttgtgatcga tgtcagccac gccagcgacg acgtggtcga
      121 ccagtcggtg gccctgtcca aggtcccgat catcgcctcg cactcggggc ccaaggcgat
      181 ctacgatcat gcccgcaaca tggacgatgc gcgcctgaag aagatcgccg acgccggcgg
      241 cgcgatctgc atcaactcga tctatctgac caacaccacg cccagcccgg agcgcaaggc
      301 cgcactggag gccctgggtc gcgggcctga cctgaagacg gcgacgcccg aggtggtcaa
      361 ggcctatgcc gacaagcgcg ccgcgatcga caaggcctat ccgcctgtgc gcggcgactt
      421 cgacctgttc atgaagagca tgttgcacgt gctcaaggtg acgggcccca agggcgtttg
      481 cgtgggcgcc gactgggacg gcggcggcgg catggacggc ttcgaggaca tcaccgacct
      541 gcccaaggtc accgcgcgcc tgaaggccga gggctattcg gacgccgaca tcgaaggcat
      601 ctggagtggc aacgtgctgc gtatcctggc ggccgccgaa gcctatgcca agagcttagc
      661 gaagtaggtt ggcgtaagac gcgccctctc ctggcagccg ccaggagagg ctatgtccgc
      721 ttgacccgat cccatatccg ccagctcatc agcaccgcga acaccgccgc atagatcagc
      781 ggcggacggt ggtcggcctt caccagcagg tagtagtggg ccacgcccag cgggacgatc
      841 agatagatca gccagtgcag ccgctgccag gtcgcccggc ccatgcggat cacccagccg
      901 ttggtcgagg tcacggccag cgggatcagc agcaggaacg cacccatgcc cagggtgatg
      961 aagggccgct tgaggatatc cttccacagc tggccaaggt cgaaaaagag atcgacgccc
     1021 acatagctga acagatgcag cacgatatag ctgaaggcga acagccccac cgtgcgccgg
     1081 aagcgcacca ggcggggcat cctgaggatc cgagccaggg gcgtgatcgc caggccaacc
     1141 agcagcagac gcagccccca gacgcccagt tggcggatca gggtctcgat cggattggcg
     1201 ccgagatcgc cggtgaagcc tcgccacgcc agccagacca gcggcgccag gcaggccagc
     1261 cacaccaggc cataaacgag gttgtccaac agtttcgacg ggcgtttgcg cgccggcttg
     1321 gccccaatca tcagaagttc ttcttcaggt ccatgccgcg ataaaggtcg gcgacgtact
     1381 ggccatagcc gttgaacggc agggtctcgc gacgacggaa ctcgccgatg cggcgctcgc
     1441 tggcctgcga ccagcgcggg tggtccacgg cgggattgac gttggaatag aagccgtatt
     1501 cgcgcggcgc cgagacgttc caggcggtgc gcggctgctt ctcggtcagg gtgatccgga
     1561 cgatcgactt gattcccttg aagccatatt tccaaggcac
//
LOCUS       DAOMEO010000838         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7889_length_1599_cov_2.365285, whole genome shotgun sequence.
ACCESSION   DAOMEO010000838 DAOMEO010000000
VERSION     DAOMEO010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_1599_cov_2.365285"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1350
                     /locus_tag="PLH31_15965"
     CDS             <1..>1350
                     /locus_tag="PLH31_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740166.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR90724.1"
                     /translation="EAHLRTLTVMGFPSQTWPGLLDDLNRLAFDYRWVTRAICLDKTD
                     AAKVLGRIRRQWFAKRKSIAAMLKEIMTNEASALMDTDAANKAADADLALQELGSDLV
                     GTAYVTATVTVWDKDPRIAEERLRLVEKVIQGRDFTCMAERVNAVEAWLGSLPGHVYA
                     NVRQPPLSTLNLAHMMPFSAVWAGPERDEHFDAPPLFLAKTEGSTPFRFSLHVGDVGH
                     TLVVGPTGAGKSVLLALMALQFRRYAGAQVFAFDFGGSIRAAALAMGGDWHDLGGSLA
                     EDAAEPVTLQPLAGIDGDDERAWAAEWVAAVLAREKVDVTPDVKELVWSALNNLASAP
                     KAERTLTGLSVLLQSQALKQALKPFCVGGPWGRLLDAEAERIGEADVLAFETEGLIGT
                     GAAGPVLAYLFHRIERRLDGRPTLLIVDEGWLALDDQGFASQLREWLKTLRKKNASVV
                     "
     assembly_gap    1351..1360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1361..>1599
                     /locus_tag="PLH31_15970"
     CDS             <1361..>1599
                     /locus_tag="PLH31_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90725.1"
                     /translation="VFATQSLADIDGSVIAPAIIESCPTRLFLPNERALEPQIAAIYR
                     RFGLNERQIEILSRATPKRDYYCQSRRGNRLFELG"
BASE COUNT          253 a          548 c          523 g          265 t
ORIGIN      
        1 agaggctcat ctacgcacgc tgacggtgat gggttttccc tcgcagacct ggccaggcct
       61 gctggacgac ctcaatcgcc tcgccttcga ctatcgctgg gtgacgcggg cgatctgcct
      121 ggacaagacc gacgcggcca aggtgctcgg ccgtatccgg cgccagtggt tcgccaagcg
      181 caagtcgatc gcggccatgc tcaaggagat catgaccaac gaggcctcgg ccttgatgga
      241 taccgacgcc gcgaacaagg cggccgacgc cgatctggcg ctccaggaat tgggatccga
      301 tctggtcggc acggcctatg tcaccgccac cgtgacggtg tgggacaagg acccgcgcat
      361 cgccgaggaa cggctgcggc tggtcgagaa ggtcattcag ggccgcgact tcacctgcat
      421 ggccgagcgg gtcaacgcgg tcgaagcctg gctcggcagc ctgcccggtc acgtctacgc
      481 caacgtccgc cagcccccgc tctcaacgct gaacctggcg cacatgatgc cgttttcggc
      541 ggtctgggcc gggccggaac gggacgagca tttcgatgcg ccgccgctct tcctcgccaa
      601 gacggaaggg tcgacgccgt ttcgcttctc gctccatgtc ggcgacgtcg gccacacctt
      661 ggtggtaggg cctaccggcg cgggcaagtc ggtgctgctg gcgctgatgg cgctccagtt
      721 tcgccgctat gccggggctc aggtctttgc cttcgatttc ggcggatcga tccgggcggc
      781 tgcgctcgcc atgggcggcg actggcatga cctcggcgga tcgcttgccg aggacgccgc
      841 cgaacccgtg accttgcaac cgctggccgg gatcgatggc gacgacgagc gcgcctgggc
      901 ggccgagtgg gtcgccgcgg ttctcgcgcg cgaaaaggtg gacgtcacgc cggacgtgaa
      961 ggagctggtc tggtccgccc tcaacaatct cgcctccgcc ccgaaggccg aacgcaccct
     1021 gacggggcta tcggtcctgc tgcaaagcca ggccctcaag caggcgctca agcccttctg
     1081 cgttggcggc ccctggggac gcctgctgga cgctgaagcc gagcggatcg gcgaggccga
     1141 tgtgctggcc ttcgaaaccg aaggcctgat cggcaccggc gccgccgggc cggtgctcgc
     1201 ctatctcttc caccgcatcg agcgccggct cgacggccga ccgaccctgc tgatcgtgga
     1261 cgaaggctgg ctggccctgg acgaccaggg cttcgccagc cagctgcgcg agtggctgaa
     1321 gacgctgcgc aagaagaatg cgagcgtcgt nnnnnnnnnn gtcttcgcca cccagtcgct
     1381 cgccgacatt gacggctcgg tcatcgcccc agccatcatt gagagctgcc cgacgcggct
     1441 ctttctgccg aacgagcgcg cgctcgagcc gcagatcgcc gccatctacc ggcgcttcgg
     1501 cctcaatgaa cgccagatcg agattctcag ccgcgccacg cccaagcgcg actattactg
     1561 ccagtctcgc cgcggcaatc gcctgttcga actgggctt
//
LOCUS       DAOMEO010000839         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7894_length_1598_cov_2.879456, whole genome shotgun sequence.
ACCESSION   DAOMEO010000839 DAOMEO010000000
VERSION     DAOMEO010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7894_length_1598_cov_2.879456"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLH31_15975"
     CDS             complement(<1..282)
                     /locus_tag="PLH31_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR90726.1"
                     /translation="MPRLTNLDLDLVRTFLVVCEQKSFTRAGGRLGRSQSAISLQVRR
                     LEDQLGQSLLSRDPRHVVLTEHGEAFLPQARRLLRLNDDILAGLAADDVE"
     gene            400..1386
                     /locus_tag="PLH31_15980"
     CDS             400..1386
                     /locus_tag="PLH31_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HQR90727.1"
                     /translation="MDIITQTLAAAGTNLLQPAVLFFALGVLAALARSDLALPREAAK
                     TLSLVLMLCIGFKGGVEARAHGLDAGFLKAAGLGLALSAIMPLPAYFLLRRAGLNTVT
                     AAATAAHYGSVSVVTFAAGQSYLTSIDQAPGGYMSAVLALMETPALLTAIAIAVFSDR
                     RGTDRPPIGRLTHEVLLNAASVILVGSFIIGLLTGPAGGAKLAVFSGPVFQGVLCLFL
                     LDLGAKAGRHLANARGMDGRVLAMGLILPLLGSAIGLAVGRLAGLGAGDLAALAVLAG
                     SASYIAAPAAMGLALPKADAGIYLTLSLGVTFPFNLALGIPLYSLIAHWLTA"
BASE COUNT          239 a          565 c          522 g          272 t
ORIGIN      
        1 ctcgacatca tcagccgcca ggcccgcgag gatgtcgtcg ttcaatcgca acaggcggcg
       61 ggcctggggc agaaaagcct cgccatgctc ggtcaagacg acgtgacggg gatcccgcga
      121 gagcagagac tggccaagct ggtcctccag acggcgcacc tgcagactga tggccgactg
      181 tgaccgcccc aggcgcccgc cggcgcgcgt gaagctcttc tgctcacaca ccaccaggaa
      241 ggtccgcaca agatccaggt ccagattggt caggcgaggc atgtcagctc catgatgatg
      301 atttgtgatg atacaaatga ccaaaatcaa caatggaaat cgatcaacac cccgtagagc
      361 ggccgcgcgc cggtcgggcg caccgggtcg gagcgcttcg tggacatcat cacccaaact
      421 ctcgccgctg cggggaccaa ccttctgcag ccggcggtgc tgtttttcgc cctgggcgtt
      481 ctggccgccc tggcgcgctc ggacctggcc ttgccgcgcg aggcggccaa gaccctctcc
      541 ctggtcctga tgctctgcat cggcttcaag ggcggcgtgg aggcgcgggc ccatggcctg
      601 gacgccggct tcctcaaggc tgcgggcctg ggcctggccc tcagcgccat catgcccctg
      661 cccgcctatt ttctgctgcg tcgggccgga cttaacacgg tgacggccgc cgcgacggcc
      721 gcccactacg ggtcggtctc ggtggtgacc ttcgcggccg gacagagtta cctgacctcg
      781 atcgaccagg cgccgggcgg ctacatgtcg gcggtcctgg ccctgatgga gaccccggcc
      841 ctgctgacgg ccatcgccat cgccgtgttc agcgatcggc gggggacaga ccggcctccc
      901 atcgggcgac taacccatga ggtgctgctg aacgccgcca gtgtcatcct ggtgggcagc
      961 ttcatcattg gcctgcttac cggccccgcc gggggcgcga agctggcggt gttctccggg
     1021 ccagtgttcc agggggtgct gtgcctgttc ctgctggatc tgggggccaa ggccgggcgg
     1081 catctggcca atgcccgggg tatggatggc cgggtgctgg ccatgggcct gatccttccg
     1141 ctcctgggga gcgcgatcgg cctggccgtc gggcgactgg ccgggctggg ggccggcgac
     1201 cttgccgccc tggccgtgct cgccggctcg gcctcctata tcgccgcgcc ggcggcgatg
     1261 ggtctggccc tgccgaaggc ggacgcgggg atctatctga ccctgtccct gggcgtgacc
     1321 tttccgttca atctggccct gggcataccg ctctacagcc tgatcgccca ttggctgacc
     1381 gcgtgaggcc ggccctgtcc cacaggcggc tgcgacagcg tcgcctcgcc tgtccccgtc
     1441 aagggacggg gacaggccca tctcgccccc aacacattca acgtcctgac cccgccaccc
     1501 agcgtcacga tgcaaaatcg acgcagcccc ctccaaaacg cccgcatact tctcctcgcc
     1561 ccgtcactgg ggaggccgtc cagctggcga cctgacgg
//
LOCUS       DAOMEO010000840         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7913_length_1593_cov_2.714564, whole genome shotgun sequence.
ACCESSION   DAOMEO010000840 DAOMEO010000000
VERSION     DAOMEO010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7913_length_1593_cov_2.714564"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..299
                     /locus_tag="PLH31_15985"
     CDS             78..299
                     /locus_tag="PLH31_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HQR90728.1"
                     /translation="MQALSIAAAGMTAAAHRLTESAQRTATWSAREIDTDLTKEAVEQ
                     VSAKNDFAANVAVARTADEMTGALLDMKV"
     gene            420..>812
                     /locus_tag="PLH31_15990"
     CDS             420..>812
                     /locus_tag="PLH31_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90729.1"
                     /translation="MIVPLKLAGLEGEQDMFRCVLAVAVAAGLLLGGAAQAAQAADSV
                     EAMMARIEKLKLQGRKGESLLRPGYAVGEYTGGATVRSKNASAGFNDSKVYSKDQMSA
                     DFTVTAPGFATPVTGSCSGGQGRIALVWI"
     assembly_gap    813..822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <823..1140
                     /locus_tag="PLH31_15995"
     CDS             <823..1140
                     /locus_tag="PLH31_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90730.1"
                     /translation="KANLTLAMSKRKGLAGMTAPQRAGEFRYDGVTLRVRTQRVGGMP
                     IGGGGALGYVFSRDGVDIGGVSLSGLAPTFYLPPKGSPDRDAAAVLALSLFFFKDPGF
                     NGR"
     gene            1214..1543
                     /locus_tag="PLH31_16000"
     CDS             1214..1543
                     /locus_tag="PLH31_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQR90731.1"
                     /translation="MSKAPDTAIKTVRTDWSQVVLVCRKCSKKLDGGFGEDGDQSLRK
                     ALRRAAGGGKGRKASIGVIEVGCFDLCPRNAVVAANASTPGQWLVVPRGTPIAAVSEQ
                     LGLMAPA"
BASE COUNT          247 a          538 c          551 g          247 t
ORIGIN      
        1 cggtgttcac ctttctttta cgcgagggct taaccgaagg cgtgggaaga cggggcgtac
       61 tgaaggaacc gcctcccatg caggctctct ccatcgccgc cgccggcatg accgccgccg
      121 cccaccgcct caccgaaagc gcccagcgca cggcgacgtg gagcgcgcgc gagatcgaca
      181 ccgatctgac caaggaagcc gtcgagcagg tctcggccaa gaatgacttc gccgccaacg
      241 tcgcggtcgc caggaccgcc gacgaaatga ccggcgcgct gctggacatg aaggtctagc
      301 gcgccgcctt ccagacgccc cgcccgcttc cccccagcgg aagggcgtct ggctgccgct
      361 ccccgggtct ccgtcccaag ttcacccggg gagcggcttg accttttccc gcttcgggcg
      421 tgatcgttcc actcaagctt gcgggccttg agggggaaca ggacatgttc aggtgcgtac
      481 tcgcggtcgc cgtggcggcc ggattgttgc tgggcggcgc ggcccaggcg gcccaggccg
      541 ccgatagcgt cgaggccatg atggcccgca tcgagaagct caagctccag ggtcgcaagg
      601 gcgaaagcct gctgcggcca ggctatgcgg tcggcgaata taccggcggg gcgaccgtgc
      661 gctccaagaa cgccagcgcc ggcttcaacg actccaaggt ctattcgaaa gaccagatga
      721 gcgccgactt caccgtcacc gcgcccggct tcgccacgcc cgtcaccggc tcgtgctcgg
      781 gcggccaggg caggatcgcc ctggtctgga tcnnnnnnnn nnaaggccaa cctgacgctg
      841 gctatgtcga agcggaaagg cctggccggc atgaccgcgc cgcagcgggc cggcgagttc
      901 cgctatgacg gcgtcaccct gcgcgttcgc acccagcggg tcggcggcat gccgatcggc
      961 gggggcgggg ccctgggcta cgtcttcagc cgcgacggcg tcgacatcgg cggcgtcagc
     1021 ctgagcggcc tggcgccgac cttctatctg ccgcccaagg gctcgcccga tcgcgacgcg
     1081 gcggcggtgc tggcgctgag cctgttcttc ttcaaggacc caggcttcaa cggacgctga
     1141 cgcggggttt tgcgcctggg tcctgccttc ctatctgtgg cgggcggcgc ccctcgccgc
     1201 gtttgaggcg atcatgagca aggcccccga cacggcgatc aagacggtca ggacggactg
     1261 gtcccaggtg gtgctggtct gccgcaaatg ttcgaagaag ctggacggcg gctttggcga
     1321 ggatggcgac cagagcctgc gcaaggccct gcggcgcgcg gccggcggcg gcaagggtcg
     1381 caaggccagc atcggcgtca tcgaggtggg ctgtttcgac ctttgcccca ggaacgcggt
     1441 ggtggccgcc aacgcctcga cgccgggcca gtggctggtc gtgccgcgcg gaacgccgat
     1501 cgctgccgtg agcgagcagc ttggactgat ggcgccggcc tagagccctt tccgacctga
     1561 tggcttcatc agggtcgggc gaaaagggct cta
//
LOCUS       DAOMEO010000841         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7916_length_1593_cov_1.769831, whole genome shotgun sequence.
ACCESSION   DAOMEO010000841 DAOMEO010000000
VERSION     DAOMEO010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7916_length_1593_cov_1.769831"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLH31_16005"
     CDS             <1..694
                     /locus_tag="PLH31_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90732.1"
                     /translation="LIPGGGYERVVLDKEGYETARWLADRGYTCFVLFYRLPGDGWAP
                     GPDVPLQDAQRGLRLIRSRAPALGINPDRVAVMGFSAGGHVAASLTTRFAAKVYQRID
                     AADDLSARPSLSALIYPVITMDPASMHGGSRQQLLGAHPTPEQIALYSPERQIGADLP
                     PVFLLHAADDKAVPVENSLMMFSALKARAIPTEMHIFEEGGHGFGLRFVTGKPVAAWP
                     GLFETFAKRHGV"
     gene            complement(691..1404)
                     /locus_tag="PLH31_16010"
     CDS             complement(691..1404)
                     /locus_tag="PLH31_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HQR90733.1"
                     /translation="MRRAERLFQIIQILRRSHAPVTADAIAVELETSKRSVYRDIAAL
                     VGQRAPIRGEAGVGYVLEAGFDMPPLMLTPDEIEAAVLGAQWVAGRGDPVLAKAARDL
                     ISKIAAAVPERLRPYVLEPATGASPAWNAAPDGLDIAQARAWIHAGRKIRLDYRDEQG
                     AVSQRVIWPVTVGYRETVRMLIGWCELRQDFRTFRTDRVVGAEFLEDRHGQRPGQLRS
                     RWQKHIEAERAAWSKQNSD"
BASE COUNT          226 a          542 c          550 g          275 t
ORIGIN      
        1 gctgatcccg ggcggcggct atgagcgggt ggtgctggac aaggaaggct acgagaccgc
       61 ccgctggctg gccgatcgcg gctatacctg ctttgtgctg ttctatcgtc tgccgggcga
      121 tggctgggcg ccgggccctg acgtcccgct gcaggacgcc cagcgcggtc tgaggctgat
      181 ccggtctcgg gcccccgccc tgggcatcaa tcccgaccgt gtggccgtca tgggcttctc
      241 cgccggcggt cacgtcgccg ccagcctgac cacgcgcttt gccgccaagg tctatcagcg
      301 catcgacgcc gccgacgacc tttcagcccg gcccagcctg tcggccttga tctatccggt
      361 gatcacgatg gatccggcct ccatgcacgg cggttcgcgc cagcagcttc tgggcgccca
      421 tccgactccg gaacagatcg cgctctattc gccggaacga cagatcggcg ccgacctgcc
      481 gccggttttc ctgctgcacg ccgccgacga caaggccgta ccggtcgaaa actcgctgat
      541 gatgttctca gcgctgaagg ccagggctat cccgaccgag atgcacatct tcgaggaggg
      601 cggccacggc tttggtctgc gcttcgtcac cggcaagccg gtcgccgcct ggccgggcct
      661 gttcgagacc ttcgccaagc ggcacggcgt ttagtcgcta ttctgtttgg accacgcggc
      721 ccgttcggcc tcaatgtgct tctgccaccg gctgcgcagc tggcctgggc gctgtccgtg
      781 gcggtcttcc aggaactcgg cgccgaccac gcggtcagtg cggaaggtgc ggaagtcctg
      841 gcgcagttcg caccagccga tcagcatgcg gacggtttcg cgatagccca ccgtgaccgg
      901 ccagatcacc cgctggctga ccgcgccctg ctcgtcgcga tagtccagcc ggatcttgcg
      961 gccagcatgg atccaggcgc gggcctgggc gatgtccagc ccgtctggcg cagcgttcca
     1021 cgcgggcgag gcgccggtgg ccggctccag cacatagggc cgcaagcgct cgggcacggc
     1081 ggctgcgatc ttgctgatca ggtcgcgcgc ggctttggcc agcacgggat cgccgcgtcc
     1141 ggccacccat tgggcgccca gcacggcggc ttcgatctca tcgggtgtga gcatcaacgg
     1201 cggcatgtcg aatccggcct ccagcacgta gccgacgccc gcttcgcccc ggatcggcgc
     1261 ccgctgtccc accagggcgg cgatgtcgcg atagacgctg cgcttggagg tctcgagttc
     1321 gacagcaatg gcgtcggcgg tgaccggcgc atggctgcgg cgcaggatct ggatgatctg
     1381 gaacaggcgt tcggcgcgtc tcatcaggac accatggcgg agctatgctg acagcatgct
     1441 ggcagtaggg tggcggtagc aaggcctgat cggacgccgg ggcgcgcaga gcgctatggc
     1501 ccccctatgg gattggggcg ccgcgcacca cgcagcgaaa gccgatgtgg ctggtggtgg
     1561 tgtcgatcgc ctgggggtga cgcgcggccg gtc
//
LOCUS       DAOMEO010000842         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7956_length_1586_cov_2.478119, whole genome shotgun sequence.
ACCESSION   DAOMEO010000842 DAOMEO010000000
VERSION     DAOMEO010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_1586_cov_2.478119"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLH31_16015"
     CDS             complement(<1..837)
                     /locus_tag="PLH31_16015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR90734.1"
                     /translation="MRGAALDNGVSVETVKVSELGAPERARWNALRQANPLLGSPYFD
                     LRFVEAAGEIAPGAAVAVIRQGGEILGFLPFQRRGTLVQPLAAPMSDIHGLVAPVDTG
                     LNLTAILSQLGADRARFGGLIGQGVSEACGLSVRYAMAADVSAGFAAYEAGRSAGFLK
                     DKRRRLRMLERDHGPVSFSFEKPAAAMLDMIVAGKRQQLHRTHQYDIFGCGWTETLLR
                     RLAEVSETDFGLKLAVLRAGDVVVAAELGMTSGDRHHLWFPIYDAAYARYSPGSLMTL
                     ETL"
     gene            complement(874..1586)
                     /locus_tag="PLH31_16020"
                     /pseudo
     CDS             complement(874..1586)
                     /locus_tag="PLH31_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662780.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
BASE COUNT          288 a          525 c          525 g          248 t
ORIGIN      
        1 cagggtctcc agggtcatca ggctgcccgg cgaatagcgc gcataggctg cgtcatagat
       61 cgggaaccac aggtgatgcc ggtccccgct ggtcatgccc aactcggcgg ccacaacgac
      121 gtcgcccgcc ctcaggaccg ctagcttcag gccaaagtcg gtctccgaga cctcggccaa
      181 acggcgcagc agggtttcgg tccagccaca gccgaagatg tcatactggt gggttcgatg
      241 aagctgctga cgcttgccgg cgacaatcat gtccagcatc gcggcggccg gcttttcaaa
      301 gctgaagctg accgggccgt ggtcgcgttc gagcatccgg agccggcggc gcttgtcctt
      361 gagaaagcca gcgctgcgcc ccgcttcata ggctgcgaaa ccggccgaaa cgtcggcggc
      421 catggcgtag cgcaccgaaa ggccgcaggc ctcgctgacg ccctggccaa tcagtccgcc
      481 gaaccgcgcc cggtcagcgc caagttggct cagaatggcc gtcaggttca ggccggtgtc
      541 gaccggcgca acaaggccat gaatgtcgct catcggcgcc gccaggggct gaaccagggt
      601 tccgcgccgc tggaaaggca gaaagcccag gatctcgccg ccttgccgga tgacggcgac
      661 cgcggcgcct ggcgcgatct cgccggcggc ttcaacgaaa cgcaggtcga aataggggct
      721 gcccagcagg ggattggcct ggcgcagggc gttccagcgg gcgcgctccg gcgcgcccaa
      781 ctccgagacc ttgaccgtct ctacggaaac cccgttgtcc aaagccgccc cccgcatcac
      841 tgcaggttac gcaggctttc cctaataatc gctttagttg agcttttccg gttcagcggc
      901 gctcaggcgc accggaaccg acttgtaggc cggcgtgccg ctacgggtgt cgcggtcgtc
      961 aagatcgacc aggatattgg tctccgggta gtaggcggcc aggcacccgc ggggaatgtc
     1021 gcgcgccacg agggtgaagc cccgcaccac gcgctctccg ccgcgatcaa aggcggcggc
     1081 gagatcaacg cgatcacccg atgcgagacc gcgggccgcg atatcctcag gatgggcgaa
     1141 caccacgtcg cggcggccaa aaaccccgcg atagcggtcg ttctggccat agatggtggt
     1201 gttgtactga tcgtggctgc gcagggtggt gaggatcatc acgtcaggat caccctggcg
     1261 cggatcgccg gccccgggcg catggaccag gaaattggcc ctgcccgagg cggtcttcca
     1321 gacccgatcc gacggcggta cgggcaggcg gaagccgcca gggacgcgga tgcgctcatt
     1381 ataattgtcg aacgttcccg gaaatacgcg ggcgatcagg tcccggatcc ggtcgtagtc
     1441 ggcgaccagg cccgaccagt cgatggcgtc cccctcgccc agaacggccc gcgcgatgcc
     1501 ggccactatg gccggctcgg atttcaggtg ttcagaggct ggcgggctca ggccccggga
     1561 cgcatgaacc atctacatcg agtcct
//
LOCUS       DAOMEO010000843         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7961_length_1585_cov_2.063399, whole genome shotgun sequence.
ACCESSION   DAOMEO010000843 DAOMEO010000000
VERSION     DAOMEO010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7961_length_1585_cov_2.063399"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..72)
                     /locus_tag="PLH31_16025"
     CDS             complement(<1..72)
                     /locus_tag="PLH31_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90735.1"
                     /translation="MDFLTLPVVGKPLWMWAGFMLAVV"
     gene            complement(168..353)
                     /locus_tag="PLH31_16030"
     CDS             complement(168..353)
                     /locus_tag="PLH31_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90736.1"
                     /translation="MRIGGYTPPTAWVSLQLAQQALVQAASHASQPTAKPVPPPDPLE
                     RAPASRPLNSGKLDIRV"
     gene            complement(350..808)
                     /locus_tag="PLH31_16035"
     CDS             complement(350..808)
                     /locus_tag="PLH31_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HQR90737.1"
                     /translation="MSLNPVHAARSRITDAWTPSQLEHLDALVAACALVSHADGWVTA
                     EERARLLERMRGLAVFAVFGVEDALEAFEALDQRFEQDPEAARFEAEMAVRRLRGRDD
                     AARVVVNAACAVAVADGGFDAEERDALLRICKLLGLDPIQFELITARGSR"
     gene            complement(1054..>1585)
                     /locus_tag="PLH31_16040"
     CDS             complement(1054..>1585)
                     /locus_tag="PLH31_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR90738.1"
                     /translation="PRVALTVTCDLTLNLLDRLREGALDIALVKREPLGPDLGVRVWR
                     EPLVWVGLDSDIVRRDGPMPLVAAPSPCVYRKRAVAALEASGRSWRATYTSPSLAGQL
                     AALRGGLGVSVLPREMAPDDLTILTGDLPQLQDAEIALIKARGGALPAAERLADYILS
                     ALDRQRLEPLPKAAGA"
BASE COUNT          272 a          535 c          544 g          234 t
ORIGIN      
        1 gacgaccgcc agcatgaagc ccgcccacat ccagagcggt ttgccgacta ccggcagggt
       61 cagaaagtcc atggtttcgg ctcacgtgca ggggaccgcc gcaacacggc gtcaaagccg
      121 gtcgcggcgg gcgcagtctc aggagattgg ggtgggtgga ggctcgctca gacccggatg
      181 tcgagcttgc cgctgttcag gggccgtgac gccggcgccc gctccagggg atcgggcggt
      241 ggcaccggct tggccgtggg ctggctggcg tgggacgccg cctgaaccag cgcctgttgc
      301 gccagctgaa gcgaaaccca ggcggttgga ggggtgtagc caccgattct catcgcgacc
      361 cccgggcggt gatcagctcg aactggatcg ggtcgagacc cagcagcttg cagatccgca
      421 gcagggcgtc gcgctcctcg gcgtcgaagc cgccatcggc caccgccacg gcgcaggccg
      481 cgttgacgac aacccgggcc gcatcgtcgc gtccgcgcag gcggcggacc gccatttccg
      541 cctcgaaccg ggcggcttcc ggatcctgct caaagcgttg gtcaagcgct tcaaaggcct
      601 ccagcgcgtc ctcgacgcca aagacggcaa agaccgccag gccccgcatc ctttccagca
      661 gtcgcgcccg ctcctcggcg gtgacccagc catcggcgtg ggacaccagc gcgcaggcgg
      721 cgaccagagc gtccaaatgc tccagttgcg acggtgtcca ggcatcggtg atgcgcgaac
      781 gcgcggcgtg cacaggattc aaagacatgg ttttgctctc ctctgttggg agggcgcgcc
      841 cgatatcggg cttgctgggc tctgggggaa caaccctctg gacccggcat cgagcgcacc
      901 cgatgcggtc cgacatcacg atcgcgccgt acggcgtcgc gtttcgtcag aggggcccgg
      961 cccgctccag acagattgga ctcaaaacgc gccggcgcaa ggccatagtc gattgatctt
     1021 cggcatgccg ttgatgagat attgaaatga tgactaggcg cctgcggcct tgggcaaggg
     1081 ctcaagcctc tgacgatcca gggcggagag gatgtaatcc gccagccgct cggcggccgg
     1141 caaggcgccg ccccgcgcct tgatcagggc gatctcggca tcctgcaact ggggcaggtc
     1201 cccggtcagg atcgtgaggt catccggcgc catctcgcgc ggcagcacgc tgacacctag
     1261 cccgccccgc agcgcggcca gctgtccggc cagggagggg ctggtatagg tggcgcgcca
     1321 gctgcggcca ctggcttcca gcgcggcgac cgcgcgtttg cgatagacgc agggcgaagg
     1381 cgcggcgacc agcggcatgg gtccgtcacg acggacgata tcagagtcca gacccaccca
     1441 gaccagcggc tctctccaga cccggacgcc cagatcgggc cccagcggct cacgtttgac
     1501 cagggcgatg tccagggctc cctcacgaag ccggtcgagc aggttaagcg tcagatcaca
     1561 ggtgaccgtc agggccaccc tgggg
//
LOCUS       DAOMEO010000844         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7962_length_1585_cov_2.051634, whole genome shotgun sequence.
ACCESSION   DAOMEO010000844 DAOMEO010000000
VERSION     DAOMEO010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_1585_cov_2.051634"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="PLH31_16045"
     CDS             complement(<1..1465)
                     /locus_tag="PLH31_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90739.1"
                     /translation="MITFDTSVLLGYYQARTGQLNGVSATAVSPSRSKAVVPSAPWLS
                     GTAEPSDLVKAALNGRKFVDEAGNATSLKGASGDYKKLFATYQALNTLSAIAARASEK
                     GVTDSELKRLQTALTKGLSEVTAYTQNMTLDQGRLTPGAVMATDRSTVGVPKNVYGYI
                     TDTIYSGDLDDEVPKFQGNVSFDLAVKKFGVTTNVTMNLLDMGATPRTMSNVVSFMNG
                     KLKAEGFETSFAVERKVGEARTVQVNGQPVTLPATGDDFALRVKGDSSEQLTFTATTA
                     SPAVYITTTAGNPDPDKDTKTDDAVIENTLTKYSAAGGGQPGGKVFSEELQGTISSVR
                     KTVAGADGSIYMLADVTKDVSGQVIKGDQDVALLKYDSAGHLLYARSLGATDSASGLN
                     LAVADDGSVAVAGSVTGRLQGAVDGPINSDATSGKSDSFVTRYDAKGDEQWTVRRGGM
                     LEDEATAVAFGSDGILYVGGRSKSDLPGSTSMAGGGYD"
BASE COUNT          299 a          512 c          460 g          314 t
ORIGIN      
        1 aatcgtagcc gccacccgcc attgacgtag agcccggcaa gtcagacttg gagcgtccgc
       61 caacataaag aatgccatca gagccgaacg cgacagccgt cgcttcatcc tccagcatac
      121 cgccgcgccg cacggtccac tgctcatcgc ccttggcgtc gtagcgcgtg acgaaactgt
      181 cgctcttgcc cgaagtggca tcggagttga tcggcccgtc gaccgcgccc tgcagccgac
      241 ctgtcacaga accggcgaca gcaacgctac cgtcgtcggc gaccgccagg ttcaagccgc
      301 tcgcggaatc cgtggccccc aggctgcggg cgtacagcag atgacccgcc gagtcgtatt
      361 tgaggagcgc gacgtcctgg tcgcccttga tcacctggcc gctaacatcc ttggtgacat
      421 ccgcgagcat gtagatcgag ccatcagctc cggccaccgt cttgcgaacg ctggagatcg
      481 tgccctgcaa ctcctcggaa aacaccttac cgcccggctg accgccccct gcggcgctgt
      541 acttggtcag cgtgttctcg atgacggcgt cgtctgtctt ggtgtccttg tccggatcgg
      601 gattgccggc ggtcgtggta atgtaaacag cgggtgacgc cgtcgtggcg gtaaaggtca
      661 attgctcgga cgaatcaccc ttcacccgaa gcgcaaagtc gtcgccggtc gcgggcaagg
      721 tcacgggctg gccattgacc tgaaccgtgc gggcctcgcc aaccttacgc tcgacagcaa
      781 agctggtctc gaagccctcg gccttcagct tgccgttcat gaagctgacg acgttcgaca
      841 tcgtccgcgg cgtcgctccc atgtccagca gattcatggt cacattggtc gtcacgccga
      901 acttcttgac ggcaagatca aagctgacat tgccctggaa cttgggcact tcgtcgtcca
      961 gatcaccact atagattgtg tcggtgatat agccgtagac gttctttggc accccaaccg
     1021 tcgagcgatc ggtagccatt accgcgccag gcgtcagccg gccctgatcc agcgtcatgt
     1081 tctgggtgta ggccgtgact tcggaaagcc ccttggtcag ggctgtctgc aatcgcttga
     1141 gctcgctgtc ggtaacaccc ttttccgacg ccctggcggc gatcgcggaa agggtgttga
     1201 gcgcctgata agtagcgaac agcttcttgt agtcgccact agcgcccttc agactggtcg
     1261 cattgccagc ttcatcgacg aatttacgac cattcaaggc cgccttgacc agatcgctgg
     1321 gctccgccgt tccagacagc cagggagccg agggcaccac agccttggac cgcgacggag
     1381 acaccgccgt ggcggacacg ccgttcagct ggcccgtgcg agcctgataa tagccgagca
     1441 gtacgctggt gtcgaaagtg atcacggcga gtcctgtcgg gatacgcgga gcaccccgtt
     1501 tctatttgaa cgcgtaacgc tgaagactcg ttaacaccct tggttacgcc gcgattaggt
     1561 gtgcttcagc caggctacga cgggc
//
LOCUS       DAOMEO010000845         1584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7967_length_1584_cov_2.525180, whole genome shotgun sequence.
ACCESSION   DAOMEO010000845 DAOMEO010000000
VERSION     DAOMEO010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7967_length_1584_cov_2.525180"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>416
                     /locus_tag="PLH31_16050"
     CDS             <1..>416
                     /locus_tag="PLH31_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR90740.1"
                     /translation="DLLLLDVKLPRLDGFAVLARLRAHSDTPVIMVTALGEDLDRLAS
                     LRIGADDHVVKPFNPLEVVARVRAVLRRTLTSAAQVDSAGRCLRVDGLEVDLVSHTAR
                     LMVADGARPAPLDLTLTEFRLLAHMARHASRVFTRG"
     assembly_gap    417..426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            481..552
                     /locus_tag="PLH31_16055"
     CDS             481..552
                     /locus_tag="PLH31_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90741.1"
                     /translation="METAGATGFLESVRGVGYRLRLP"
     gene            549..>1584
                     /locus_tag="PLH31_16060"
     CDS             549..>1584
                     /locus_tag="PLH31_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR90742.1"
                     /translation="MSPPSNATSPSLKGSGLRTRLVRTLVVMVGLTVILGFVLVQGAV
                     VLASYHHLPNIPPDLLAAEKALRAGAVPDRDTILRMIEAGEETSLIEDRAILLSLGVL
                     LVLSALSGAAIALHLSRLIGTPVERVAVAARRLAGGDFTARAEPSPGSAQETAQLVAD
                     FNHMAQALETLDRELNENAAAIAHELRTPLTILRGRLQGLRDAVFPPTPREFDGLIGQ
                     VEALTRLVEDLRTVSLATTGRLELRRGAVDLAVELDLLLDGMEPILAASGLTLERDLR
                     PAPLEADGERLRQATLALLENARQHAGGCVRVETQWLQPIGAQATVVLRVLDRGPGIA
                     ADPPDLVFGRF"
BASE COUNT          221 a          524 c          563 g          266 t
ORIGIN      
        1 ggacctgttg ctgctggacg tcaagctgcc gcggctcgac ggatttgcgg tgctggcgcg
       61 ccttcgcgct cacagcgaca caccggtcat catggttacg gcgctcggcg aggatctgga
      121 caggctggcc agtctgcgga tcggcgccga tgaccacgtg gtcaagccgt tcaacccgct
      181 ggaggtggtg gcccgagtcc gggcggtgtt gcggcgaacc ctgaccagcg ccgcccaggt
      241 cgacagcgcc ggacgctgcc tgcgggtcga cggcctggag gtggaccttg tctcccacac
      301 agcccgactg atggtggccg acggcgctcg gccggcgccg ctggacctga ccctgacgga
      361 attccggctc ctggcgcata tggcgcgcca tgccagccgg gtctttacac gcggggnnnn
      421 nnnnnngggg acgcgctgga gcgcacggtc gacagccatc tgagcaagct gcgccgcaag
      481 ctggaaaccg cgggggccac agggtttctg gaaagcgtac gcggcgtcgg ctaccggctg
      541 aggctgccgt gagcccgcct tccaacgcca cctcgccctc cctgaaagga tctggcctgc
      601 gcacccgact ggtccgcacc ctcgtggtga tggtcgggct caccgtgatt ctgggcttcg
      661 tactggtgca gggcgcggtt gtactggcca gctaccacca tctgcccaat atcccgcccg
      721 atctgctggc cgccgagaag gctttgcggg cgggcgccgt gcctgatcgc gacaccatcc
      781 tgcgcatgat cgaggcaggc gaagagacga gcctgatcga ggaccgcgcc atactgctgt
      841 cgctgggcgt gttgcttgtg ctgtcagcgt tgtccggggc ggccatagcc ctgcacctgt
      901 cgcgtctgat cggcacgccg gtggagcggg tggcggtggc ggcgcgtcgg ctggccggcg
      961 gtgatttcac cgcgcgggcc gagccgtccc ccggatcggc ccaggaaacg gcgcagctgg
     1021 tggctgactt caatcatatg gcccaggccc tggagaccct ggatcgtgag ctgaacgaga
     1081 acgccgccgc catcgcccat gagctgcgaa cgcccctgac catactgcgc ggccgactac
     1141 agggcctgcg cgacgcggtg tttcccccca cgccgcgcga gtttgacggc ctgatcgggc
     1201 aggtggaagc gctgacgcga ctggtcgagg atctgcgcac cgtcagtctg gccaccacgg
     1261 gccgtctgga gctgcgcaga ggcgcggtcg atcttgcggt ggagctggac ctgctgctgg
     1321 acggcatgga gccgatcctg gcggcgtcgg gtcttaccct ggagcgcgac ctgcgccctg
     1381 cccccctgga ggccgacggg gaaagactgc gtcaggcgac cctggccctg ctggagaacg
     1441 ctcgccagca tgccggcggc tgcgtccggg tcgagaccca gtggcttcag ccgatcgggg
     1501 cgcaggcgac ggtggttctg cgggtgctcg atcgcggtcc cggtatcgcc gccgatccgc
     1561 ctgatctggt gtttggccga tttt
//
LOCUS       DAOMEO010000846         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7975_length_1583_cov_2.087042, whole genome shotgun sequence.
ACCESSION   DAOMEO010000846 DAOMEO010000000
VERSION     DAOMEO010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_1583_cov_2.087042"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLH31_16065"
     CDS             <1..665
                     /locus_tag="PLH31_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90743.1"
                     /translation="LAVLFLVAIIGAAAWRTYALTVLQVSEVYTYKATESRIDSIAWG
                     CLAAVIERSWSTAMDVIKRRAWLVFGTGCALMLASLAYRDPVFRETLRYSVQGLALVG
                     IILPLVAAPQMGGLVKLLEIAPLRWMGRRSYAAYLWHMLALTLGGMIVGFSGVIETAP
                     RITQLQALPFVVALSWIFAELSYRFVYTPAQKLKPWVTPKRPSEANAARIPPDRAAEA
                     VS"
     gene            complement(678..>1583)
                     /locus_tag="PLH31_16070"
     CDS             complement(join(678..1278,1277..>1583))
                     /locus_tag="PLH31_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611772.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HQR90744.1"
                     /translation="AAIDGGLSCRAAAARFGVSAASAIRWRQLTLSQGDARPGPLGGD
                     RRSGLIEKNADVILAMLEAKPDITLAELQAGLAERGVAVSGSSIWRFFDRRRITLKKV
                     SAHADEQSRPDILKRRRAWFEGQLDLDPARLVFLDETGATTKMARRHGRAPRGERLRA
                     SVPHGHWKTTTFIGGLRLSGMTAPMVLDGPMTGAWFLAYVEQILVPTLAPGDIVILDN
                     LAAHKSAAARAAIEMAGARLLFLPPYSPDLNPIENAFSKLKALLRKAAARTVEQLWKA
                     IAHAIDAFTPNECANYFAAAGYDAD"
BASE COUNT          237 a          525 c          539 g          282 t
ORIGIN      
        1 ggttggctgt gctgttcctg gtcgccatca tcggcgcggc ggcatggcgg acctatgcct
       61 tgaccgtgtt gcaggtgtcg gaagtctata cctacaaggc caccgaaagc cggatcgact
      121 cgatcgcctg gggctgcctg gccgccgtca tcgaacgctc gtggtctacc gccatggatg
      181 tcatcaagcg tcgcgcctgg ctggtcttcg ggaccggctg cgccctgatg ctggcctcgc
      241 tggcctatcg cgacccggtc ttccgcgaga ccctgcgcta ttcggtgcag ggcctggccc
      301 tggtcggcat catcctgccg ctggtcgccg cgccgcagat gggcggactg gtcaagctgc
      361 tggagattgc gccgctgcgc tggatgggtc gacgatccta tgccgcctat ctgtggcaca
      421 tgctggcact gaccctgggc ggcatgatcg tgggctttag cggggtcatc gaaaccgcgc
      481 cccgcatcac ccagcttcag gccctgccct tcgtggtggc cctgtcgtgg atcttcgccg
      541 agctcagcta tcgcttcgtc tataccccgg cccagaagct gaagccgtgg gtcacgccca
      601 aacgccccag cgaggcgaac gccgcgcgga tccccccgga ccgcgcggcc gaggcggtca
      661 gctagagcat tttccgatca gtctgcatca tagccggcgg cggcgaagta gttggcgcat
      721 tcgttgggtg tgaaggcgtc gatggcgtgg gcgatggcct tccaaagctg ctcgacggtt
      781 cgggcggcgg cttttcgcag cagggccttg agctttgaga aggcgttctc gatcggattg
      841 aggtctgggc tgtagggcgg caggaacagt agccgggcgc cggccatctc gatcgcggcc
      901 cgggcggcgg cgcttttgtg ggcggccaga ttgtcgagga tcacgatatc gccaggcgcc
      961 agcgtgggga cgaggatctg ttcgacatag gccaggaacc aggcgccggt catggggccg
     1021 tccaggacca tgggcgcggt catgcccgac aggcgcagac cgccgatgaa ggtcgtggtc
     1081 ttccagtggc cgtggggcac gctggcccgc agacgttcac cgcgaggcgc gcggccgtgc
     1141 cgacgagcca tcttggtggt agctccggtc tcgtccagga ataccagccg ggccgggtcc
     1201 agatccagct gaccctcgaa ccaggcccgc cggcgcttca ggatatcggg acggctctgt
     1261 tcgtccgcgt gcgccgactt ttttgagcgt aatccgccgc cggtcgaaga accgccagat
     1321 cgacgagccg ctgaccgcca cgccgcgctc ggccaagccg gcctggagct cggccagggt
     1381 gatgtccggc ttggcctcaa gcatcgccaa gatcacgtcg gcgttcttct cgatgagacc
     1441 ggaacgacga tcacctccaa gaggccccgg gcgcgcatcg ccctggctca gggtcagttg
     1501 ccgccatcgg atcgcgctgg ccgcgctgac cccaaaccgc gccgccgccg cgcggcacga
     1561 cagcccacca tcgatcgcgg cga
//
LOCUS       DAOMEO010000847         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_7980_length_1582_cov_2.420432, whole genome shotgun sequence.
ACCESSION   DAOMEO010000847 DAOMEO010000000
VERSION     DAOMEO010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_1582_cov_2.420432"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>289)
                     /locus_tag="PLH31_16075"
     CDS             complement(<1..>289)
                     /locus_tag="PLH31_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR90745.1"
                     /translation="PGEFDGPAFHAHAYSDPFDPVDMRGKNVVVVGMGNSAMDIASEL
                     SQRPIAKNLWVSARRGVWVFPKYLNGKPADKSALPAWMPRKLGLSLTRKVLK"
     assembly_gap    290..299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<300..748)
                     /locus_tag="PLH31_16080"
     CDS             complement(<300..748)
                     /locus_tag="PLH31_16080"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR90746.1"
                     /translation="MTGKLPKACIIGAGCSGFTTAKRLKDYGVPYDCFEMSDEVGGNW
                     YYKNPNGLSACYESLHIDTSKWRLAFEDFPVPADWPDFPHHAQLHQYFKDYVDHFGLR
                     ETITFNTRVEKARRTDDGLWAVTLGTGETRLYDVLFVCNGHHWDPRIP"
     gene            898..1419
                     /locus_tag="PLH31_16085"
     CDS             898..1419
                     /locus_tag="PLH31_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HQR90747.1"
                     /translation="MSDSKAKTMTRHELKTWPKYFAAVRSGHKRFEIRRNDRDFSVGD
                     ILVLREFHPEDDAYTGQVEERQITFLLSEEDYGVIHGFVVIGFGELLPHPDAPVDGVL
                     TADQLAAWHETQASNAALRAADARKVSQSYAAPTTGRAAMSVAADRHAGVAAAAEAEA
                     GFHAAAAKIVRKG"
     gene            complement(1487..>1582)
                     /locus_tag="PLH31_16090"
     CDS             complement(1487..>1582)
                     /locus_tag="PLH31_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR90748.1"
                     /translation="RFIQAIISMENRLITYLRMNDVFPALISEAA"
BASE COUNT          285 a          504 c          503 g          280 t
ORIGIN      
        1 tcttcagcac cttgcgggtc agggacaggc ccagcttgcg cggcatccag gccggcaggg
       61 ccgacttgtc ggccggcttg ccgttcagat atttcggaaa cacccacacc ccgcgccggg
      121 ccgagaccca gaggttctta gcaatggggc gctgggacag ctcgctggcg atatccatgg
      181 ccgaattgcc catgcccacc accacgacgt tcttgccgcg catgtcgacg ggatcgaagg
      241 ggtcgctata ggcatgggcg tggaaggccg gaccgtcaaa ctcgccgggn nnnnnnnnng
      301 ggatgcgcgg gtcccaatgg tggccgttgc agacaaacag cacgtcgtag aggcgggtct
      361 cgccggttcc cagggtcaca gcccacaggc cgtcatcggt acggcgggcc ttctcgaccc
      421 gggtgttgaa ggtgatggtc tctctcaggc cgaaatggtc gacatagtcc ttgaagtact
      481 ggtgcaactg ggcgtggtgg gggaagtcgg gccagtcggc cgggaccggg aaatcctcga
      541 aggccagccg ccatttgctg gtgtcgatat gcagactctc gtagcaggcc gacagtccgt
      601 tggggttctt gtagtaccag ttgccgccca cctcgtcgga catctcgaag cagtcatagg
      661 ggacgccgta gtccttcaac cgcttggccg tggtgaagcc gctgcagccc gcgccgataa
      721 tgcacgcctt gggaagcttg cccgtcatgt ccgcccctga cttatcgttg ttcatgaaag
      781 cgtatcagcg ttatgcaggc ggtcaaacgg cgcccctacg tcacagcgct ccacagttgg
      841 acgttgctgg gttaatcctc cgctaggacg ttcggggaga aaccccgcga ctgatccgtg
      901 tccgactcga aagccaagac catgacccgt cacgaactga agacctggcc caaatatttc
      961 gctgccgtgc ggtcgggcca caagcggttc gagatccgcc gcaacgaccg cgacttctcg
     1021 gtcggcgaca tcctggtgct gcgcgaattc caccccgaag acgacgccta taccggccag
     1081 gtcgaggagc gtcagatcac cttcctgctg tcggaggagg actacggcgt catccacggc
     1141 ttcgtggtca tcggttttgg cgaactgctg ccgcatcccg acgccccggt cgatggcgtg
     1201 ctgacggctg accaattggc cgcctggcac gagacccaag cctccaacgc cgccctgcgc
     1261 gccgccgacg cccgcaaggt ctcgcagagc tatgcggcgc ccaccaccgg ccgcgcggcc
     1321 atgagcgtcg ccgctgatcg ccacgccggc gtcgccgctg cggccgaagc cgaggcgggt
     1381 ttccacgccg cggccgcgaa gatcgtccgc aagggttagg acacgaaaaa gccgccgccc
     1441 gcgatcagcg gacggcggct ttttagaatg cgggaaagcc ggcttcttag gccgcttcgc
     1501 tgatcagcgc cgggaacacg tcgttcatgc gcagataggt gatcaggcgg ttttccatcg
     1561 agatgatcgc ctggatgaag cg
//
LOCUS       DAOMEO010000848         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8011_length_1578_cov_2.097833, whole genome shotgun sequence.
ACCESSION   DAOMEO010000848 DAOMEO010000000
VERSION     DAOMEO010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_1578_cov_2.097833"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLH31_16095"
     CDS             complement(<1..444)
                     /locus_tag="PLH31_16095"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQR90749.1"
                     /translation="MTLPPTLSGARRVIGPVARAGERGQSQGGGRPRVSFEFFPPKTL
                     AMEESLWQAITRLAPLDPAFVSVTYGAGGSTRERTHRTVKRILDETSLKPAAHLTCVG
                     ASREEVDEVIREYWETGVRHIVSLRGDPPPTEGGIGGVYAPRADGY"
     gene            complement(441..1418)
                     /locus_tag="PLH31_16100"
     CDS             complement(441..1418)
                     /locus_tag="PLH31_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR90750.1"
                     /translation="MKLSAEQVVEVLRAAGESTRLRLLALLASEELSVLELCQILDQS
                     QPRVSRHLKLLAEAGLVERFPDGAWVFYRLASKSPGRHLVGQALDLIDDADPVVHADA
                     EKLAKVRAERSTGAQAYFARNAARWNEIRSLYVDEAEVEAAILRAAGEGPFDEMVDLG
                     AGAGRMLTLLGQRAGAALGLDLSQQMLNIARDEVAKAGLAQCELRHGDIFATGLPGGC
                     ADLVTVHQVLHYLGDPAAAVAEAARLVAQGGLLLIADFAPHDHEFLREAHQHRRLGFE
                     DAEIVPWLEAAGLRLESNIALPPSSADGLTVKIWTARRPAVRSVERSAA"
BASE COUNT          265 a          550 c          543 g          220 t
ORIGIN      
        1 atagccgtcg gcgcgcggcg catagacacc gccgatacca ccctccgtgg gcggaggatc
       61 gccgcgcagc gagacgatgt ggcgaacgcc cgtctcccag tattcgcgga tcacctcgtc
      121 cacttcctcg cggctggccc cgacgcaggt caggtgggcg gcgggcttca gcgaggtctc
      181 gtcgaggatg cgcttgaccg tgcggtgggt gcgctcgcgg gtcgagccgc cggcgccata
      241 ggtcaccgag acgaaggccg gatccagcgg cgccaggcgg gtgatggcct gccacaggct
      301 ctcttccatc gccagtgtct tgggcgggaa gaactcgaac gagacgcgcg ggcgaccgcc
      361 gccctggctc tgaccgcgct cgccggcgcg ggcgacgggt ccgatcaccc ggcgggcccc
      421 agaaagcgta ggtggaaggg tcatgcggcg ctcctttcga cggaacgaac agccggacgg
      481 cgcgccgtcc agatcttgac ggtcagccca tcggccgaag acggcggcaa ggcgatgttg
      541 ctttccaggc gcaggccggc ggcctccagc cagggaacga tctcggcgtc ctcaaagccc
      601 aggcggcgat gctggtgggc ctcgcgcagg aactcgtggt cgtgcggagc gaaatcggcg
      661 atcagcagca ggccgccctg ggccaccagc cgcgcagcct cggccacggc ggcggcggga
      721 tcgcccagat agtgcagcac ctgatggacg gtgaccagat cggcgcagcc gcccggcagg
      781 ccggtggcga agatgtcgcc atgccgtagc tcgcactgcg ccagacccgc cttggccacc
      841 tcgtcacggg cgatgttgag catctgttgc gacaggtcca ggcccagggc cgcgccggcc
      901 cgctggccca gcagggtcag catgcgtccc gcgcccgcgc ccagatcgac catctcgtcg
      961 aagggcccct cgccggccgc acgcaggatc gccgcctcga cctcggcctc gtcgacatag
     1021 agcgagcgga tctcgttcca gcgggcggcg ttgcgagcga aataggcctg ggcgccggtc
     1081 gagcgctcgg cccgcacctt ggccagcttt tcggcgtcgg cgtgaacaac gggatcggcg
     1141 tcgtcgatca gatccagcgc ctgtccgacc aggtggcgcc cgggcgactt gctggccagg
     1201 cgatagaaca cccaggcccc gtcggggaat cgctccacaa gcccggcctc ggccagcagc
     1261 ttgaggtggc gcgatacgcg cggctggctc tgatccagaa tctggcacag ctccagcacc
     1321 gaaagctcct cgctggccag cagggcgagc aggcgcaggc gcgtcgactc ccccgccgcg
     1381 cgcagcacct cgacaacctg ttcggccgac agcttcatga ggacataaag atatccttat
     1441 gtccgatttg gcaagctgaa tttgagcgcc gagggtcgag aacgatgcgg caaactatgc
     1501 cccggcggca gcctccagag gctgccagag ctcgatcttg accccggccg gatcgataag
     1561 ccaggcaaac cgccccat
//
LOCUS       DAOMEO010000849         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8019_length_1576_cov_3.155819, whole genome shotgun sequence.
ACCESSION   DAOMEO010000849 DAOMEO010000000
VERSION     DAOMEO010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_1576_cov_3.155819"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PLH31_16105"
     CDS             <1..99
                     /locus_tag="PLH31_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR90751.1"
                     /translation="RTFRGVITALSRGRGRVLAKLAAKAVKDEPKG"
     gene            153..1151
                     /locus_tag="PLH31_16110"
     CDS             153..1151
                     /locus_tag="PLH31_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HQR90752.1"
                     /translation="MTLREMTAIDADAFHGTIVPAAQPVIFRGLVSAWPAVQAGAESD
                     EALFAYLSSFDQQQSITTLVGDPEAGGRFFYNADLTGFNFRSGGARLQKSFEFLLANR
                     DAPNPASLAVQSVPTNTHLPGFGQANPSRLLDPSVEARLWIGNRAIIAAHHDPYENLA
                     CVVAGRRRFTLFPPSSVGDLYIGPFELTPGGPTISMVSFDDPDLERHPRFARALEASL
                     VAELEPGDALYIPYLWWHHVRSLAPVNVLANYWWTPPAEARGAPHDAFLHAVLALRAL
                     PDAHRAAWKTLFDHYVFGDGDAATAHLPADRQGILGRLSPDEAQRLRRLLSQQILS"
     gene            complement(1148..>1576)
                     /locus_tag="PLH31_16115"
     CDS             complement(1148..>1576)
                     /locus_tag="PLH31_16115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR90753.1"
                     /translation="QRGWESASFSAVVDNMLGHYGKRYQRAGQDVSLAPSVVIILGMA
                     LHELATNAVKYGAWLEDGIVHIEATISDNALRVEWRERGGPPAVKPEHRGFGSRLLER
                     GVASELSGVVTLDYAASGLTCMIVAPLSDKVQPLPPEKTD"
BASE COUNT          247 a          546 c          513 g          270 t
ORIGIN      
        1 cgcaccttcc ggggcgtgat caccgcgctg tcgagaggcc ggggcagggt gctggccaag
       61 ctggcggcca aggcggtcaa ggacgagcca aagggctaaa ggtcagacca caggtcttga
      121 cgcggcgacc gccagagttg aaggcggagg ccatgaccct tcgtgaaatg accgcgatcg
      181 acgccgacgc gttccacggg acgatcgtcc cggcggcgca gccggtgatt ttccggggac
      241 tggtctccgc ctggcccgcc gtccaggcgg gggcggaaag cgacgaggcc ctgtttgcct
      301 atctgtccag cttcgaccag caacagtcga tcacgaccct ggtcggggat ccggaggccg
      361 gcggccggtt cttctacaac gccgatctca cgggcttcaa ttttcgcagc ggcggcgcac
      421 ggctgcagaa gagctttgag ttcctgctgg ccaatcggga cgcgccgaac cccgccagcc
      481 tggcggtgca gtcggtgccg accaacaccc atctgccggg gtttgggcag gccaatccct
      541 cccgcctgct ggacccctct gtcgaggctc ggctgtggat cggcaaccgg gcgatcatcg
      601 ccgcccatca cgacccctat gagaacctgg cctgcgtggt ggccgggcgg cggcgcttca
      661 ccctgttccc gcccagcagc gtgggcgatc tctatatcgg cccgttcgaa ctgacgccgg
      721 gcggtccgac gatcagcatg gtgtcattcg atgaccctga ccttgaacgt catccccgct
      781 tcgccagggc gctggaggcc tccctggtgg ccgagctgga gccgggcgac gcgctctata
      841 ttccctatct ctggtggcac catgttcgct cactggcccc ggtgaacgtg ttggccaact
      901 actggtggac gccgcccgca gaggcgcggg gcgcgcccca tgacgccttc cttcacgcgg
      961 tgctggcgct gcgcgccctg ccagacgccc acagggcggc ctggaaaacg ctctttgacc
     1021 actatgtgtt tggcgatgga gacgcggcga cagcgcacct gccggccgac cgccagggca
     1081 tattgggccg cctctcgccg gatgaggccc agcgcctgcg ccgcctgctg agccagcaga
     1141 tcctgtccta gtctgtcttt tccgggggta gcggctgcac cttgtccgat agcggagcca
     1201 cgatcatgca ggtcaggccc gaggcggcat agtccagcgt aaccaccccc gacagctccg
     1261 aggcgacccc ccgttccagc aggcgtgatc caaaacccct gtgctcgggc ttgacggcgg
     1321 gcggcccgcc gcgctcacgc cactcaacgc gaagcgcatt gtcgctgatc gtggcttcga
     1381 tatggacgat cccgtcttcc agccacgcgc cgtacttgac ggcgttcgtc gccagttcat
     1441 gcagggccat gcccagaatg atgaccacgc tgggcgccag cgagacgtcc tgcccggcgc
     1501 gctggtagcg tttgccgtag tgacccagca tgttgtcgac cacggcgctg aatgaggcgc
     1561 tctcccagcc gcgctg
//
LOCUS       DAOMEO010000850         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8040_length_1574_cov_2.502304, whole genome shotgun sequence.
ACCESSION   DAOMEO010000850 DAOMEO010000000
VERSION     DAOMEO010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8040_length_1574_cov_2.502304"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="purM"
                     /locus_tag="PLH31_16120"
     CDS             complement(<1..916)
                     /gene="purM"
                     /locus_tag="PLH31_16120"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQR90754.1"
                     /translation="MSDLPNGLTYAQAGVDIDAGNALVEAIKPLAKATRRPGADGGLG
                     GFGALFDLKAAGYDDPLLVSTTDGVGTKLRIAIDAKMHATVGIDLVAMCVNDLLAQGA
                     EPLLFLDYFATGKLDLDVATSVVAGIAEGCKLAGAALVGGETAEMPGMYSDGEYDLAG
                     FSVGAVERDGVLPKLDKQRAGDMIIGIGSSGPHSNGYSLVRRVVERSGLAWDAPAPFE
                     AGKTLAEALMAPTRIYVKSILPLLQSGRVKGGAHITGGGLIENPPRCIAAGLVPEFDW
                     NAWPLPPVFAWLQEVGGISDHELRRTFNC"
     gene            1031..>1574
                     /locus_tag="PLH31_16125"
     CDS             1031..>1574
                     /locus_tag="PLH31_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQR90755.1"
                     /translation="MKPITTTAELAAFCNALQGQPFVAVDTEFMRETTYWPKLCLIQV
                     GAPNIEAVIDPLADGLDMTPLLDLMRDERVLKVFHAARQDVEIFNNLGAMPRPLFDTQ
                     VAGMAAGFGEQIAYDALVRQMLKIELDKSSRFTDWARRPLSEAQLTYALADVTHLASL
                     FPMLRQRLADAGRLAWVEEEM"
BASE COUNT          258 a          526 c          525 g          265 t
ORIGIN      
        1 cgcagttgaa ggtgcggcgc agttcgtggt cggaaatgcc gcccacttcc tgcagccagg
       61 caaagaccgg cggcaggggc caggcgttcc agtcgaactc cggaaccagg cctgccgcga
      121 tgcagcgtgg cgggttctcg atcaggccgc cgccggtgat gtgggcgccg cccttgaccc
      181 gtccggactg cagcaagggc aggatcgact tcacatagat gcgggtgggg gccatcaggg
      241 cctcggccag ggtcttgccg gcttcgaagg gcgccggggc gtcccaggcc aggcccgagc
      301 gctcgaccac gcggcgcacc agcgaatagc cgttggagtg cggacccgac gagccgatgc
      361 cgatgatcat gtcgccggcc cgttgcttgt ccagcttggg cagcacgccg tcgcgctcga
      421 ccgcgccgac cgagaagccg gccagatcgt actcgccgtc gctgtacatg cccggcattt
      481 cggcagtctc gccgccgacc agggccgcgc cggccagctt gcagccttcg gcgatgccgg
      541 ccaccacgct ggtggcgaca tcgagatcca gcttgccggt ggcgaaatag tcgaggaaca
      601 acaagggctc ggcgccttgg gccagcaggt cattgacgca catggccacc aggtcgatcc
      661 cgaccgtggc gtgcatcttg gcgtcgatgg cgatgcgcag cttggtgccg acgccatcgg
      721 tggtggagac cagcagcggg tcatcatagc cggccgcctt caggtcgaag agcgcgccga
      781 aaccgcccag accgccgtcg gcaccagggc gtcgggtggc cttggccagg ggcttgatcg
      841 cctcgaccaa agcgttgccg gcatcgatgt cgacgcccgc ctgggcgtag gtgaggccgt
      901 tcggcagatc gctcattcgc tcgctctggt ctatttgagg acgcagttgc gccttcaact
      961 cgcggaaatc tgcaccccgg ctatgcttga cgcttgcaga ctcgggggtg cgcgaggcta
     1021 ttagcagccg atgaagccga taaccactac cgccgagctg gcggcgtttt gtaatgcgtt
     1081 gcagggccag cccttcgtcg ccgtggacac agagttcatg cgcgagacga cctattggcc
     1141 caagctctgc ctgatccagg ttggcgcgcc caatatcgag gccgtgatcg atccgcttgc
     1201 ggacggcctc gacatgacgc cgctgctgga tctgatgcgc gatgagcgcg tgctgaaggt
     1261 gttccacgcc gcccgccagg acgtcgaaat cttcaacaac ctgggcgcca tgccccggcc
     1321 gttgttcgac acccaggtcg ccggcatggc cgccggcttt ggcgagcaga tcgcctacga
     1381 cgctctagtg cgacagatgc tcaagatcga gctcgacaag tccagccggt tcaccgactg
     1441 ggcccgtcgc ccgctgtccg aagcccagct gacctacgcc ctggccgatg tcacccactt
     1501 ggcctcgctg tttccgatgc tgcgccagcg cttggccgac gccggccgcc tggcctgggt
     1561 cgaggaagag atgc
//
LOCUS       DAOMEO010000851         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8041_length_1574_cov_2.392363, whole genome shotgun sequence.
ACCESSION   DAOMEO010000851 DAOMEO010000000
VERSION     DAOMEO010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_1574_cov_2.392363"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1574)
                     /gene="dsbD"
                     /locus_tag="PLH31_16130"
     CDS             complement(115..>1574)
                     /gene="dsbD"
                     /locus_tag="PLH31_16130"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQR90756.1"
                     /translation="QSDVAAREADAQTAQAAAMLAIQAPGAAPAPENDEGAAAHSPVA
                     SSPAVTLDANPGFVDRLALQGGATWVLLAFFGFGVLLAFTPCVFPMYPILAGVIGRGV
                     DGRGTRRGLTLSVAYVLGLSIAFALLGVAAAWSGQNLQMALQSVWAVGALAVVFVILA
                     ASMFGGFELQLPSTWTSRLSRDGGDGRRSLPSAAGLGFVSALIVGPCVTAPLAGALLY
                     IAQTGDVGLGAAALFFLGLGKGVPLIVFGTVGARFLPKAGPWMDRVKMLFGFIFLGMA
                     WWLASRILPPNATLALGAALALGLAAALGLFQPLSPNLRNGVARVAGLAAATWGLLLL
                     VGLSLGADDPWRPLEPLARPAGVAAAAPVETAAVVLDQTALDRAVADAGARERPALVY
                     FTADWCVSCRVIERTVFEDPAVIAGLSNTDLIKVDVTRSTPEARDLLERHGVVGPPTM
                     IFLSPTATEAPGSRLIGEMSAEDVASALQSAGAAA"
BASE COUNT          277 a          551 c          577 g          169 t
ORIGIN      
        1 agcgtgggct gacccggctg gccagcagtc cggcgccgat catgaagacg aacacgccgg
       61 cgatgatggc gaaccgctcg cccgacagga cgagcgggcc cagggtgatg gcgttcatgc
      121 ggcggctccg gcgctctgca gggcggatgc gacgtcttcg gccgacattt ctccgatcag
      181 ccggctgccc ggtgcctcgg tcgccgtggg cgacaggaag atcatcgtcg gcggtccgac
      241 cactccgtgc cgctcaagaa gatcgcgcgc ctcgggggtg ctgcgcgtaa cgtcgacctt
      301 gatgaggtcg gtgttcgaaa ggccggcgat gacggcgggg tcctcgaaca ccgtccgctc
      361 gatcaccctg cagctgacgc accagtcggc ggtgaaatag accagggcgg gacgttcgcg
      421 cgcgccggca tcggcgacgg cgcggtccag cgccgtctga tccagcacga ccgccgcggt
      481 ttcgaccggc gcggcggctg cgactccagc gggacgcgcc aggggctcaa gcggacgcca
      541 cgggtcgtcc gccccgagac tgagtccgac caacagcaac aggccccagg tcgcggccgc
      601 caggccggcg acgcgggcga cgccgtttct caggttcggg ctcagcggct ggaacaggcc
      661 gagggccgcc gccagaccga gcgccagcgc cgcgccgaga gccagcgtcg cgttcggcgg
      721 caggatgcgg ctggccagcc accaggccat gcccaggaag atgaagccga acagcatctt
      781 cacccggtcc atccacggcc cggccttggg caggaagcgc gcgccgaccg tgccgaacac
      841 gatcaagggc acccccttgc ccaggcccag gaagaacagg gccgccgcgc ccagcccaac
      901 gtcgccggtc tgggcgatgt agagcagggc gcccgccagg ggcgcggtga cgcaaggccc
      961 cacgataagg gcggagacga agcccagacc tgcggctgag ggcagggacc gccggccatc
     1021 gccgccgtcg cgggagaggc ggctggtcca ggtcgagggc agttgcaact cgaagccgcc
     1081 gaacatggag gcggcgagga tgacgaagac gacagccaga gcgcccacgg cccaaaccga
     1141 ctggagcgcc atctgcaggt tctgtccgga ccaggcggcc gcgacgccca ggagggcgaa
     1201 ggcgatggaa aggcccagca cataggcgac ggagagcgtc aggcctcggc gcgtaccgcg
     1261 cccgtccacg ccgcgtccga tgacgccggc caggatcgga tacatgggaa agacgcaggg
     1321 cgtgaaggcc agcaggacac caaagccgaa gaaggcgagc aggacccagg tcgccccacc
     1381 ctgcagcgcc aggcgatcga cgaagcccgg gttggcgtcc agagtgaccg ccggcgagga
     1441 cgcgaccggc gaatgcgccg ccgccccctc gtcgttctcg ggcgcgggtg cagcgcccgg
     1501 cgcctggatc gccagcatgg ccgcggcctg tgcggtctgc gcatcggcct cccgcgcggc
     1561 cacgtcggac tgcg
//
LOCUS       DAOMEO010000852         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8055_length_1571_cov_2.083113, whole genome shotgun sequence.
ACCESSION   DAOMEO010000852 DAOMEO010000000
VERSION     DAOMEO010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8055_length_1571_cov_2.083113"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLH31_16135"
     CDS             <1..1092
                     /locus_tag="PLH31_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90757.1"
                     /translation="QFELSRVPDFMPRLLPDSWLGGAALLLIVFVMSTILDNIAAAVI
                     GAVIARKVYAGRVSLGFLAAIVACSNAGGAGSVIGDTTTTMMWIAGVSPLVVLKAFIA
                     SGVAFVIVAGFAAAAQHRYQPIVAHVAGDTPPVDGLRMLVVVLLLAALLGTNFVGNAY
                     FPSQQGAAPWLGLGLWGAILVTQILRKPDWSVMPEAGRGAAFLVCLVGLASLMPVDSL
                     PPASWPSALGLGFLSAVFDNIPLTALALKQGGYDWGVLAFAVGFGGSMMWFGSSAGVA
                     VSNLFPEARSVGGWLKQGWFVPVGYVAGFLVLLLAQGWVPTATRTQSEGVGVEAADHH
                     FDPNAAARFWGDARGLVDRALAEAAQRGR"
     gene            1164..>1571
                     /locus_tag="PLH31_16140"
     CDS             1164..>1571
                     /locus_tag="PLH31_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR90758.1"
                     /translation="MKTRKLGDGLEVSAIGLGCMGMSWAYGPAPEKAETFKVLARAVE
                     LGVTFFDTAEVYGPFVNEELVGEGLKPFRDKVVIATKFGFKIAAPDENGVRRMVGVDS
                     RPEHIREVAEASLKRLGVEIIDLLYQHRVDPNVP"
BASE COUNT          197 a          507 c          577 g          290 t
ORIGIN      
        1 cagttcgagc tgagccgggt tcccgatttc atgccgcggc tgctgcccga cagctggctg
       61 ggcggcgcgg cgctgctgct gatcgtcttt gtgatgtcga cgatcctcga caatatcgcc
      121 gccgccgtga tcggggcggt gatcgcccgc aaggtctatg cagggcgggt cagcctgggc
      181 tttctggcgg ccatcgtggc ctgttcgaac gcgggcgggg ccggcagcgt gatcggcgac
      241 accaccacca ccatgatgtg gatcgccggc gtctcgcccc tggtggtgct caaggccttc
      301 atcgcatccg gcgtcgcgtt cgtgatcgtc gccggctttg cggcggcggc ccagcatcgg
      361 taccagccaa tcgtcgccca tgttgcgggc gacaccccgc ccgtggacgg cctgcggatg
      421 ttggtcgtgg tcctgctgct ggccgcgctg ctggggacca acttcgtcgg caacgcctat
      481 ttccccagcc agcagggcgc cgcgccctgg ctgggtctgg ggctgtgggg cgcgatcctg
      541 gtcacccaga tccttcgcaa gcccgactgg tcagtgatgc ccgaggcggg gcgcggggcg
      601 gcgttcctgg tgtgtctggt cggcctggcc tcgctgatgc cggtcgactc gctgccgccg
      661 gcctcgtggc ccagcgcgct ggggctcggc ttcctgtcgg cggtgttcga caatatcccg
      721 ctgacggccc tggcgctcaa gcagggcggc tatgactggg gcgtgctggc ctttgcggtc
      781 ggctttggcg gttcgatgat gtggtttggc tcctcggcgg gcgttgcggt gtccaacctg
      841 ttccccgagg cgcgctcggt gggcggctgg ctgaaacagg gctggtttgt tccggtcggc
      901 tatgtggcgg gcttcctggt tctgctgctg gcccagggct gggttcccac agccacgcgg
      961 acccagagcg agggcgtcgg cgtcgaggcc gccgatcatc atttcgatcc gaacgccgcc
     1021 gccaggttct ggggggatgc gcggggcctg gtggaccggg cgctggcgga agccgcgcag
     1081 cgggggcggt agccgcagag ggggcctcct caagggagtg tcgggcgcct atgttgggga
     1141 accacgacat caggagctgc cccatgaaga cccggaaact cggcgacggt ctggaggtct
     1201 cggccatcgg cctgggctgc atggggatga gctgggctta cggccccgcg cccgaaaagg
     1261 ccgagacctt caaggtgctg gcccgcgccg tcgagctggg cgtcaccttc ttcgacaccg
     1321 ccgaggtcta tgggccgttc gtcaatgagg agttggttgg cgaggggctg aagcccttcc
     1381 gcgacaaggt ggtgatcgcc accaagttcg gcttcaagat cgcggcgccg gacgagaacg
     1441 gcgtgcgccg catggtcggc gtcgacagcc gccccgagca catccgcgag gtcgccgagg
     1501 cctcgctcaa gcgcctgggc gtcgagatca tcgacctgct gtaccagcac cgcgtcgacc
     1561 ccaatgtgcc c
//
LOCUS       DAOMEO010000853         1569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8075_length_1569_cov_2.129458, whole genome shotgun sequence.
ACCESSION   DAOMEO010000853 DAOMEO010000000
VERSION     DAOMEO010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8075_length_1569_cov_2.129458"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLH31_16145"
     CDS             complement(<1..491)
                     /locus_tag="PLH31_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90759.1"
                     /translation="MRGNITIDPLARATAVADPQIREVRSLKSGQILEARRFIESVRY
                     DRLVQVRSQIKEAMDAGSPRARLRDLRNGGLHRLQPRQGVLLPASSRGWLLPAQTRDG
                     LSEDDIRTMKYKGAQESEAHRRLKALIERSLVADPRFQGVLAEKVWRGQRDPASLRRP
                     DIQA"
     gene            780..>1569
                     /locus_tag="PLH31_16150"
     CDS             780..>1569
                     /locus_tag="PLH31_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HQR90760.1"
                     /translation="MTRSASRSRGRTDRANIYDEITSRIIGELEAGRLPWVQHWGSSA
                     VAAPLAMPRNAATGRGYSGINLLILWGAVVQHGYASQGWITFRQALGLGGNVRKGEHG
                     TTVVYADRFIPDDERRRARETGDEAAAIPFLKRFTVFNIDQCEGLPEELSAPPPPVPE
                     GLILPEAEALLRASGADLRLGGDKAFYAPGPDYIQIPRPEAYFEPVNWHRTALHELGH
                     WSGHPSRLARDLSGGFGSKAYAREELVAEMTSAFCCASLGITPTV"
BASE COUNT          230 a          550 c          538 g          251 t
ORIGIN      
        1 gcttggatgt ccggccgcct gagcgaggcg ggatcgcgct gaccgcgcca gaccttctcc
       61 gccagaacgc cctggaaacg cggatctgcg accagcgagc gttcgatcag ggccttcagg
      121 cggcggtgcg cctcgctttc ctgggcgccc ttgtacttca tggtccgaat atcgtcttcg
      181 ctcaggccgt cgcgggtctg ggccggcagc aaccatcctc gactcgatgc cggaagaaga
      241 acgccttgtc ggggctggag acgatgtaga ccgccgttcc gcagatcgcg caggcgggcg
      301 cgcgggctgc cggcatccat ggcttccttg atctggctgc gcacctgcac gagacggtcg
      361 tagcgcacgc tttcgatgaa gcgacgcgcc tccaggatct gccccgactt caggctgcgg
      421 acctctcgga tctgcgggtc cgccaccgcc gtggcgcggg ccagcggatc gatcgtgatg
      481 ttgccgcgca tagcccctct tgacgccctc ggttgctcct cttgggttgc ggccgataga
      541 tcgcgccggt tcacgtgagg gcctggaggg gaaagccttc ccctgcggcc gaatctgaag
      601 tctcggccga ccccttctgc ggggggagcc ccgcaacgcc cctcggtggc gccggcgccg
      661 gtcaggcagg cggaaggcgg aaggctccgc ggtcgagagg gagagggccg cggctgtcgc
      721 cgtgacgggt tttgaaggtc gagagaggag ctccggccgg cccgtcgcgg agacatgcga
      781 tgacacgctc agcttccagg agccgcggcc ggaccgatcg ggccaacatc tatgacgaaa
      841 tcaccagccg gatcatcggc gagctggagg ctggccgcct gccctgggtc cagcactggg
      901 gctccagcgc cgtagccgcg ccgctcgcca tgcccaggaa cgccgccacg ggccgcggct
      961 attcggggat caacctcctg atcctgtggg gcgcggtcgt ccagcacggc tatgcgagtc
     1021 aaggctggat caccttccgt caggcgctcg gcctgggcgg caatgtgcgc aagggcgaac
     1081 acgggaccac ggtcgtctac gccgatcgct tcattcccga cgacgagcgc cggcgcgccc
     1141 gtgagacggg cgacgaggcg gccgccatcc cgttcctcaa gcgcttcacc gtcttcaaca
     1201 tcgaccagtg cgagggcctg cccgaagagc tctccgcccc gccgccgccg gtcccggaag
     1261 ggctgatcct tcccgaggcc gaggccctgc tgcgggccag cggcgccgat ctgcgcctgg
     1321 gcggcgacaa ggccttctat gccccgggcc cggattacat ccagatccct cgccccgagg
     1381 cctatttcga gccggtcaac tggcaccgga cggccttgca cgagctgggt cactggtcgg
     1441 gtcaccccag ccggctggcc cgcgacctga gcggagggtt cggctccaag gcctatgccc
     1501 gcgaggagct ggtggccgag atgacctcgg ccttctgttg cgccagcctc ggcatcacgc
     1561 cgaccgtgc
//
LOCUS       DAOMEO010000854         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8098_length_1566_cov_2.444739, whole genome shotgun sequence.
ACCESSION   DAOMEO010000854 DAOMEO010000000
VERSION     DAOMEO010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_1566_cov_2.444739"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLH31_16155"
     CDS             complement(<1..413)
                     /locus_tag="PLH31_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQR90761.1"
                     /translation="MADAYIFDAVRTPRGKGKKDGSLHEITSLSLASQVLEALRDRNN
                     LDTSYVDDVVLGCVAPVGEQGSDIARTAVLTADYAESVAGVQINRFCASGLEAVNMAA
                     AKVASGEAQLAIGGGVESMSRVPMGSDGGAWPTDPS"
     gene            complement(437..916)
                     /locus_tag="PLH31_16160"
     CDS             complement(437..916)
                     /locus_tag="PLH31_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQR90762.1"
                     /translation="MSTHTATISWALKPEEDFPKGRYSRVHTLSFDGGVTLTGSASPS
                     VVPLPWSDPAGVDPEEMFVAALSACHMLTFLDLARRAGFHITAYADTAEGVMTKNDHG
                     AHWVSTVTLRPVITWQDAAPSEAAEAVLHEAAHHACFIANSVRTDVRVEPALRLQPV"
     gene            complement(1104..1460)
                     /locus_tag="PLH31_16165"
     CDS             complement(1104..1460)
                     /locus_tag="PLH31_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90763.1"
                     /translation="MMAQAAAPAAKPAPDARAAVVCVRADRAGRRPLTFAGQLVGKRP
                     QPGAFKLPLTPGADVCNNLLRSKIATWTLEVTTSGFTPCTLPPPEFGYRVTYSAKATR
                     GLQCAQIAKSPIGTWK"
     gene            complement(1475..>1566)
                     /locus_tag="PLH31_16170"
     CDS             complement(1475..>1566)
                     /locus_tag="PLH31_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR90764.1"
                     /translation="IARMYRDSRVQRIYGGTNEVMKILIARTL"
BASE COUNT          246 a          501 c          561 g          258 t
ORIGIN      
        1 gaggggtcgg tcggccaggc gccgccgtcg ctgcccatgg gcacgcggct catgctttcg
       61 acgccgccgc cgatggccag ctgggcctcg ccggaggcca ccttggcggc cgccatgttc
      121 acagcttcca ggcccgaggc gcagaagcgg ttgatctgca cgccggcgac gctctcggca
      181 tagtcggcgg tcagcacggc ggtgcgggcg atgtcgctgc cctgctcgcc cacgggggcg
      241 acgcagccca gcaccacgtc atcgacatag gacgtgtcga ggttgttgcg gtcgcggagg
      301 gcttccagga cctggctggc cagggacagc gaagtgatct cgtgcagaga tccgtctttc
      361 ttgcccttgc cgcgcggggt gcgcacggcg tcaaagatgt aagcgtcggc catctgatgg
      421 ctccttcttg ggacgtctaa acgggttgga ggcggagcgc cggctcgacc cgcacatccg
      481 tgcggaccga attggcgatg aaacaggcgt ggtgggcggc ctcgtgcagg accgcctcgg
      541 cggcctcgct gggggctgca tcctgccagg tgatcacggg gcgcagcgtc acggtgctga
      601 cccagtgcgc gccatggtca tttttcgtca tcaccccttc tgccgtgtcg gcataggcgg
      661 tgatgtggaa gccggcccgg cgggccaggt ccaggaaggt cagcatgtgg caggccgaca
      721 gggccgcgac gaacatctct tcaggatcga cgccggcggg gtctgaccac ggcagcggca
      781 ccaccgacgg cgaggccgag ccagtcagcg tcacgccgcc gtcgaacgac agcgtatgga
      841 cgcggctgta gcggcccttg gggaagtctt cttccggttt cagagcccag ctgatggtcg
      901 cggtgtgggt agacatcaga gcgcccaacc cagaatttgc cttcctgggc cttgtgccca
      961 ggatccatgc tcaaggcagg cggagcccat cagctcttgc ccgccgcaag gtccagcgca
     1021 cggctctaga tcagcgaggg cggaaccatg ggtcctgggc acaaggccca ggaaggcggg
     1081 atctggttga tgggagcggt tcatcacttc caggttccga tgggcgactt ggcgatctgg
     1141 gcgcattgca aaccccgcgt cgccttggcg ctgtaggtca cccgatagcc aaactccggc
     1201 ggcggcaggg tgcagggtgt gaagcccgag gtcgtgacct ccagtgtcca ggtggcgatc
     1261 ttcgaccgca gcaggttgtt gcagacatcg gcgccgggcg tcagcggcag cttgaacgcg
     1321 cccggctgcg ggcgcttgcc cacaagctgg cccgcgaagg tcaggggccg gcggccggcg
     1381 cggtcagcgc gcacgcagac cacagccgcg cgggcgtcag gagcgggctt ggcggccggc
     1441 gctgcggcct gagccatcat caagacgagg tcgatcacag cgtcctcgcg atcaggatct
     1501 tcatcacctc gttggtgccg ccatagatcc gctggacgcg gctgtcgcgg tacatccggg
     1561 cgatcg
//
LOCUS       DAOMEO010000855         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8099_length_1566_cov_2.441430, whole genome shotgun sequence.
ACCESSION   DAOMEO010000855 DAOMEO010000000
VERSION     DAOMEO010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_1566_cov_2.441430"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..982)
                     /gene="tsf"
                     /locus_tag="PLH31_16175"
     CDS             complement(44..982)
                     /gene="tsf"
                     /locus_tag="PLH31_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQR90765.1"
                     /translation="MAEITAALVKELREKSGVGMMDCKKALQENAGDIDASIDWLRAK
                     GLSKAAKKADRVAAEGLVGVAVRNDGAGMTAAAVEVNAATDFLGRNDLFQSAVRKIAA
                     VGLDNEGVDAINAGKTSDGEVVSDVLTNLIATIGENMVLRRSARFSVAEGAIATYIHN
                     ATAPELGRIGVLVAIEGAGDQAKIVELGRKIAMHVAATSPLSLSPDDLDQAAIEKERA
                     IFTEQALESGKPAGVVEKMVEGRIRKFLEEVVLLKQAFVMNPDQTVEQLVADTGKELG
                     STLTVKGFVRLALGEGVEKAPVGDFAAEVAAMAGQA"
     gene            complement(1077..>1566)
                     /gene="rpsB"
                     /locus_tag="PLH31_16180"
     CDS             complement(1077..>1566)
                     /gene="rpsB"
                     /locus_tag="PLH31_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQR90766.1"
                     /translation="KELLQLTRERDKLELSLGGIKDMGGIPDIMFVIDTNKEAIAILE
                     ARKLNIPVIAILDTNCDPDGITYPIPGNDDAARALQLYCDLIADAVLDGLAAGQASSG
                     VDLGASVAPMEPTLARELTPEPVAAEAAAPEAIVQEAAPEAAQEATAEALEAVAEEPA
                     AS"
BASE COUNT          253 a          507 c          509 g          297 t
ORIGIN      
        1 agaaaagcca cgcgccgccg aggcgacccg cgagctcaat atcctaggcc tggccggcca
       61 tggcggcgac ttcagcggcg aaatcgccaa ccggcgcctt ctcgacgcct tcgcccagcg
      121 ccaggcgcac gaagcccttc acggtcaggg tggaacccag ttccttgcca gtgtcggcga
      181 ccagctgctc gacggtctgg tccgggttca tgacaaaggc ctgcttcagc agcacgactt
      241 cttccaggaa cttgcggata cggccttcga ccatcttctc gacgacgccg gcgggcttgc
      301 ccgattccag cgcctgctcg gtgaagatcg cgcgttcctt ttcgatggcg gcctggtcca
      361 ggtcgtccgg cgaaagcgac agcggcgacg tcgcagcgac gtgcatggcg atcttgcggc
      421 ccagttcaac gatcttggcc tggtcgccag cgccttcgat ggccaccagc acgccaatgc
      481 gacccagttc aggggccgtg gcgttatgga tgtaggtcgc gatcgcgcct tcggcgacgc
      541 tgaagcgcgc cgagcgacgc aggaccatgt tctcgccgat ggtcgcgatc aggttggtca
      601 gcacgtcaga gacgacttcg ccgtccgagg tcttgccggc attgatggcg tcgacgccct
      661 cgttgtcgag gccgacggcg gcgatcttgc ggacagcgct ctggaacagg tcgttgcgac
      721 ccaggaagtc ggttgcggcg ttcacttcga cagcggcggc ggtcatgccg gcgccgtcgt
      781 tgcgaacggc gacgcccacg aggccttcag cggcgacgcg atcggccttc ttggcggcct
      841 tggacaggcc cttggcgcgc agccagtcga tggacgcgtc gatgtcgccc gcgttctcct
      901 gcagcgcctt cttgcagtcc atcatgccga cgccggactt ttcgcgcagt tctttaacca
      961 gcgcagccgt gatctcagcc atggataatc tccatattca aacggttagc gaccgggctt
     1021 gatagcccgg tcgcatgtca gggtcgcaac agtcgcccga aggcgaccac tggaaatcag
     1081 ctcgcggccg gctcttcagc gacggcttcg agcgcttcag cggtcgcttc ttgagcggcc
     1141 tcgggagcgg cttcttgaac gatggcttcc ggggcagcgg cttcagcagc caccggctcc
     1201 ggggtcagtt cgcgagccag ggtcggttcc atcggagcaa ccgaagcgcc cagatcgacg
     1261 cctgacgagg cctggccggc ggccagacca tccagaaccg catccgcgat caggtcgcag
     1321 tacagttgca gggcgcgggc ggcgtcgtcg ttacccggga tcggataggt gatgccgtcc
     1381 ggatcgcagt tggtgtccag gatcgcgatg accgggatgt tcagcttgcg ggcttcaagg
     1441 atcgcgatcg cttccttgtt ggtgtcgatc acgaacatga tgtcggggat gccgcccatg
     1501 tccttgatgc cgccgagcga cagttccagc ttgtcgcgtt cgcgggtcag ctgcagcagt
     1561 tccttc
//
LOCUS       DAOMEO010000856         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8100_length_1566_cov_2.229649, whole genome shotgun sequence.
ACCESSION   DAOMEO010000856 DAOMEO010000000
VERSION     DAOMEO010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8100_length_1566_cov_2.229649"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLH31_16185"
     CDS             <1..674
                     /locus_tag="PLH31_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90767.1"
                     /translation="NYEFGWKTSWMENRFRWNGAVFMEDWQNFQFSILGANGLTEIKN
                     ANEARIKGVESDVTLAVTQGFTISAAATYTDAKLTANYCGFTDTSGNPVTVCPTPQAP
                     DGTVLPVTPKFKANLTARYDFSFSGYDAYLQASAVHQTGSWTDLRIVEREIIGRQSGY
                     TLVDLSGGVTRDDWSMALYVKNLTDKRASLNRYAQCAESVCGAQTYITPNQPRTIGLK
                     VGRKF"
     gene            745..>1566
                     /locus_tag="PLH31_16190"
     CDS             745..>1566
                     /locus_tag="PLH31_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR90768.1"
                     /translation="MKTSVAKPLGFWMCVALVVGNMIGSGVFMLPASLAPYGWNAVIG
                     WVVTIAGGLCLAFVFARLAKAFPKAGGPYAFTQEAFGRTAGFMVAWSYWISLWVGNAA
                     IATGTVSYLSVFFPQIATVPGLHGGITVAMVWGLTALNCYGARSAGRLQVVTTILKLM
                     PLLAVIILAFVVLAKTGGSTVTPLRMADLSAVGITSAATLTLWALLGLESATVPADKV
                     HDPERTIPRATLVGTAFTGLIYLVVCSAVVLLTPAGMLEGSNAPFADFVGLYGGGD"
BASE COUNT          246 a          527 c          503 g          290 t
ORIGIN      
        1 agaactacga gttcggctgg aagaccagct ggatggagaa ccgcttccgc tggaacggcg
       61 cggtgttcat ggaagactgg cagaacttcc agttctcgat cctgggggcc aacggcctga
      121 ccgagatcaa gaacgccaac gaggcgcgta tcaagggcgt ggaaagcgac gtgaccctgg
      181 cggtcaccca gggtttcacg atcagcgcag cggccaccta taccgacgcc aagctgacgg
      241 ccaattattg cggctttacc gacacctcgg gcaatccggt caccgtctgc cccacgccgc
      301 aggcgccgga cggcacggtc ctgccagtga cgccgaagtt caaggccaac ctgacggcgc
      361 gctacgactt cagtttcagc ggttacgacg cctatctgca ggcctcggct gtccatcaga
      421 cgggcagctg gaccgacctg cggatcgtcg agcgcgaaat catcggacgt cagtcgggct
      481 acaccctggt cgacctgtcg ggcggcgtca cgcgggacga ctggtcgatg gcgctgtacg
      541 tcaagaacct gaccgacaag cgcgccagcc tcaaccgtta cgcccagtgc gccgaaagcg
      601 tctgcggggc ccagacctac atcacgccca accagccgcg cacgatcggc ctgaaggtcg
      661 gccgcaagtt ctaggtggaa ggggcggccc gcaaggccgc ccctttttct ttctaccgtg
      721 tcgcgttcgt gaaagcctgg ccgcatgaag acttccgtcg ccaagccgct gggcttctgg
      781 atgtgcgtcg ccctggttgt gggcaacatg atcggctcgg gcgtcttcat gctgcccgcc
      841 tcgctcgcgc cctatggctg gaacgcggtg atcggctggg tggtgaccat cgccggcggc
      901 ctgtgcctgg ccttcgtctt tgcccgactg gccaaggcct ttcccaaggc cggcggcccc
      961 tatgccttta cccaggaagc gttcgggcga acggccgggt tcatggtggc ctggagctac
     1021 tggatctcgc tttgggtcgg caacgccgcc atcgccaccg gcacggtcag ctatctctcc
     1081 gtgttcttcc cgcagatcgc cacggtcccg ggcctccatg gcggcattac cgtggcgatg
     1141 gtctggggac tgacggccct caactgctac ggcgcccgct cggcgggccg gctgcaggtg
     1201 gtcaccacca tcctgaagct gatgcctctg ctggcggtga taatcctggc cttcgtggtg
     1261 ctggccaaga ccggcggcag cacggtgacc ccgctgcgca tggccgacct ttcggcggtc
     1321 ggcattacct ctgccgcgac tctgaccctt tgggcgctgc tgggcctgga gtcggccacc
     1381 gtgccggccg acaaggtgca tgatcccgag cgcaccatcc ctcgcgccac cctggtgggc
     1441 acggccttca ccgggctgat ctatctggtc gtctgctcgg cggtggtgct gctgaccccg
     1501 gccggcatgc ttgagggctc caacgccccg ttcgccgatt tcgtgggcct gtatggtggt
     1561 ggcgac
//
LOCUS       DAOMEO010000857         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8101_length_1566_cov_1.973527, whole genome shotgun sequence.
ACCESSION   DAOMEO010000857 DAOMEO010000000
VERSION     DAOMEO010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8101_length_1566_cov_1.973527"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLH31_16195"
     CDS             complement(<1..477)
                     /locus_tag="PLH31_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90769.1"
                     /translation="MLAAERLYGQHGLDGVSLRQLATAAGQANNYVVQHHFGSKLGLI
                     QAVSEMRLPAMEAHRQTLLDVARAEGDYSVSRMLSCLFSPLVTVLEAQDMKDYARFTL
                     AVMHLDPEQHPFARSTDISPASTEIRSKLSESLSHLGPDVFRRRLALSASVFLNGIS"
     gene            854..>1209
                     /locus_tag="PLH31_16200"
     CDS             854..>1209
                     /locus_tag="PLH31_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR90770.1"
                     /translation="MSHLFAPTGAASAASAALVAAAMVVGLPPSAAAAADAAPPIVST
                     TEGLVQGAPSKGVLSFLGIHYGADTGGENRFLPPAPPPSWNGVKVADRMGDRCPQPPV
                     NMPPEMASVLSFSDLPT"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1220..>1566
                     /locus_tag="PLH31_16205"
     CDS             <1220..>1566
                     /locus_tag="PLH31_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR90771.1"
                     /translation="NCLVLNVWTPAVADSAKRPVMVWLHGGGFFLGSGGDKYYEGSNL
                     SRGQDVVVVTLNHRLNAFGYLHLGSEAGPEYADSNLAGMLDIVAALKWIKANIAQFGG
                     DPNNVTIFGQSGG"
BASE COUNT          277 a          517 c          492 g          270 t
ORIGIN      
        1 ggaaatgccg ttcaggaaca cgctggcgct cagcgcgagc cggcggcgga acacatcggg
       61 tccgagatgt gacaggctct cgctaagttt gctgcggatc tcggtcgaag cgggcgaaat
      121 gtcggtggag cgcgcgaacg gatgctgctc cggatccaga tgcatgacgg ccagggtaaa
      181 ccgcgcatag tccttcatgt cctgcgcctc caacactgtc accagcggcg agaacaggca
      241 gctgagcatg cgcgagacgc tgtagtcgcc ctcggctcgc gcgacgtcga gcagggtctg
      301 acgatgcgcc tccatggcgg gcaggcgcat ctccgacacg gcctggatca agccaagctt
      361 ggagccgaaa tgatgctgga ccacatagtt gttggcctgc cccgcggccg tggccaactg
      421 gcgcagcgag accccatcaa gaccgtgctg accatatagg cgttcggcgg ccagcatcag
      481 ctttcgagcg gtcttcggat actcctggaa aagtctcggc ataggcgatc cttgcccgac
      541 tggcgtgcaa aataaaagcc gacgatccgc caaagatcgt gccgaaaatc ccgcaaacct
      601 cgacttaagt cacttgactt atattggtgt tggatctccc atgcatcggg gcgcttagtc
      661 cggcggccgc ttagcccgcc ggacgatcag acctcgaata ggggcgcgtc accgcgcgac
      721 ccgggagtgg aaacgtaggt cttcaccgtt ctcaccggct tcaccgcgcg gtcggtcatg
      781 ccgaccccgc ggccggacgg gagacggacc agcagcaatc aggccctagc ggccattcgg
      841 gaacaggttt ggcatgtctc atctcttcgc tcccaccgga gccgcgtccg cggcgtccgc
      901 cgcccttgtc gccgccgcga tggtcgtggg cctgccgccg tccgcggctg ccgcagccga
      961 cgccgcgccg ccgatcgtgt ccacgaccga ggggctggtc cagggcgcgc cctccaaagg
     1021 cgtcctatcg ttcctcggca tccactatgg ggcagacacc ggcggcgaaa accggttcct
     1081 gccgcccgcc ccgccgccgt cctggaacgg cgttaaggtc gccgaccgca tgggcgatcg
     1141 ctgtccgcag ccgcccgtga acatgccgcc ggaaatggcc agcgtgctgt cgttttcgga
     1201 cctgccgacn nnnnnnnnna aaattgcctt gtcctgaacg tctggacccc ggcggtcgcc
     1261 gacagcgcca agcgcccggt catggtgtgg ctgcatggcg ggggtttctt cctcggcagc
     1321 ggcggcgaca agtattacga gggcagcaat ctctcgcgcg gccaggacgt cgtcgtggtg
     1381 acgttgaacc accgtctgaa cgcgttcggc tatctgcacc ttgggtccga ggcgggtccc
     1441 gaatatgccg actccaacct ggccgggatg ctcgacatcg tcgcggccct caaatggatc
     1501 aaggccaata tcgctcagtt cgggggcgac cccaacaatg tcaccatctt cggtcagtcc
     1561 ggcggc
//
LOCUS       DAOMEO010000858         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8128_length_1563_cov_2.242042, whole genome shotgun sequence.
ACCESSION   DAOMEO010000858 DAOMEO010000000
VERSION     DAOMEO010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8128_length_1563_cov_2.242042"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..>904)
                     /locus_tag="PLH31_16210"
     CDS             complement(85..>904)
                     /locus_tag="PLH31_16210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR90772.1"
                     /translation="ASVEKRHSALAMLVSDFKGVPGAAEALKTNPPRLLAATPVQRIQ
                     ATRMDQERLIDNAETFAKSRAERLRLAMRMAGLDAGNYTGRGASLGGPLIEAKDPRAL
                     AAVLDVDEEFATRIHRAATDMSDMRALNQAAQKLPFFRPTSNPAMSSSYGVRFDPFTR
                     RPAFHSGLDFPGAFHTPIMATAPGVISFTGVRSGYGNTVEIDHGRGFKTRYAHLQAIS
                     VRVGERVAIGQRVGAMGSTGRSTGPHLHYEVWANGRAQNPNRFLKAGEYVQQAN"
     assembly_gap    905..914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<915..1172)
                     /locus_tag="PLH31_16215"
     CDS             complement(<915..1172)
                     /locus_tag="PLH31_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5930 domain-containing protein"
                     /protein_id="HQR90773.1"
                     /translation="MAIKRFLRLRQSLEGLFPERHLYIRSGGEMRGYVFSTNKQLLAA
                     TAVGCAALWMGVCTAAMMVNALAVSSTDQQVIKQRAYYERLN"
     gene            complement(1306..>1563)
                     /locus_tag="PLH31_16220"
     CDS             complement(1306..>1563)
                     /locus_tag="PLH31_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQR90774.1"
                     /translation="KDSAASHAKFRLKHDLTVELAADTTGEIVETYGAWVEKSMYGRK
                     YMGIDRSTFLIDADGVIREVWRKVKVPGHIKAVLTAARALK"
BASE COUNT          293 a          460 c          513 g          287 t
ORIGIN      
        1 ttcggcggcg ccttgcgggc ctggtctaca gcggcgacgg gggtaggcag gggctcgatc
       61 ttggccggac cgcgcgccgg cgttttagtt tgcttgctga acatactcac cagccttcaa
      121 aaagcgattt gggttctggg ccctaccatt ggcccagact tcatagtgca ggtgcgggcc
      181 ggtagaccga cccgtcgagc ccatggcccc aacgcgttga ccaatcgcga cccgttcgcc
      241 aacgcgcact gaaatcgctt gaaggtgagc ataacgcgtc ttgaagccgc ggccatggtc
      301 gatctcgacc gtgttaccgt agccggaccg aacaccggtg aaggagatca caccgggcgc
      361 ggtcgccatg atcggcgtgt gaaatgcgcc tgggaagtcg aggccggaat ggaaggccgg
      421 ccggcgcgta aacggatcga aacgcacccc gtagctgctg ctcatcgcgg ggttgctggt
      481 cggacggaag aagggaagct tctgggccgc ctggttcagg gcccgcatgt cagacatgtc
      541 ggtggcggcg cgatggatgc gggtggcgaa ctcctcatca acgtcaagca cggcggcaag
      601 agcgcgagga tccttggctt cgatcagcgg accgcccagc gacgcgccgc gtccggtgta
      661 gttgccggcg tcgaggccag ccatgcgcat ggccaaacgg aggcgttcgg cgcggctctt
      721 ggcaaaggtc tcggcgttgt cgatcaggcg ttcctgatcc atccgggtgg cctggatgcg
      781 ttgcactggc gtagccgcca gcagccgggg cgggttggtc ttgagggctt cggcggcgcc
      841 gggcacgccc ttgaagtcgc tgaccagcat ggccagggcc gaatggcgct tctcgaccga
      901 ggcgnnnnnn nnnngttgag gcgttcatag taggcgcgct gcttgatgac ctgctggtcg
      961 gtcgagctga ccgccagcgc attgaccatc attgcggcgg tgcagacgcc catccacagg
     1021 gcggcgcagc ccaccgcggt ggcggccaga agctgcttgt tggtggagaa aacatagccc
     1081 cgcatctcgc cgccggagcg gatgtagagg tgccgttcag ggaacaggcc ttcaagggat
     1141 tgtcgcagtc gcaagaagcg cttgatcgcc atcgcctagg aaacccccgt tttcaggaca
     1201 cggggatatg caacgataaa aaggggcggc gcaaggctct tcgcccccaa agaaatacaa
     1261 caccaatata tgtcgcaggc ccgaccttgg acgatgatga tcccgttatt taagagcgcg
     1321 cgctgctgtg agcacagcct tgatgtgtcc gggaaccttc accttgcgcc atacctcccg
     1381 aattacaccg tccgcgtcga tcagaaaggt ggaacgatcg atacccatat atttgcggcc
     1441 atacatgctc ttctcgaccc aggcaccgta ggtctcgacg atctcgcccg ttgtatcggc
     1501 agccagctca acggtcaggt catgcttgag gcggaatttg gcatgggagg cggcgctgtc
     1561 ctt
//
LOCUS       DAOMEO010000859         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8139_length_1561_cov_2.628818, whole genome shotgun sequence.
ACCESSION   DAOMEO010000859 DAOMEO010000000
VERSION     DAOMEO010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_1561_cov_2.628818"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1271)
                     /gene="tyrS"
                     /locus_tag="PLH31_16225"
     CDS             complement(48..1271)
                     /gene="tyrS"
                     /locus_tag="PLH31_16225"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQR90775.1"
                     /translation="MKPSPHPGPPVLDGPPSPHESSHPESRSMSAPASFKSEFLQTLQ
                     ARGYIHQITHPDELDAAAAGGIVTAYIGFDATGKDESRKLLSDADIAANIAGIKQVFA
                     RFLTFGDGPTDAVMVDNDEWLSKFGYVQFLRDYGVHFTVNRMLAFDSVKLRLEREQPM
                     TFLEFNYMLMQAVDFLELNRKFGCVLQMGGSDQWGNILNGVELTRRVDQKSAFGLTTP
                     LLATASGAKMGKTMSGAVWLNAEQLSPYDYWQFWRNTEDADVGRFLKLFTDLPLDEIA
                     RLEALPGAQINDAKKVLADEATRMAHGEEAAKTARDAAEKAFEQGVTSADLPTFEVPA
                     ADLKAGIVLANLFADAGLAASRGEARRLAQGGGVKLNDKAEADANRVVTEADLVDGVV
                     KLAAGKKKIVLVKPV"
BASE COUNT          260 a          487 c          532 g          282 t
ORIGIN      
        1 cgtatcgtcc cgacccgcag cggccgcgag tcgagacgac tgataaccta aaccggtttg
       61 accagaacga tcttcttctt gcccgccgcc agcttcacga cgccgtcaac cagatcagcc
      121 tcggtgacaa cccgattggc gtcggcctca gccttgtcat tgagctttac gccgccgccc
      181 tgggccaatc ggcgagcctc gccgcgcgag gcggcaaggc cagcgtcggc gaacaggttg
      241 gccaggacga tccccgcctt caagtcggcg gccgggactt cgaaggtcgg caggtcagcc
      301 gaggttacgc cttgctcgaa agccttttcg gccgcgtcgc gggcggtttt cgcggcctct
      361 tcgccgtgcg ccatgcgggt ggcttcgtct gccagcacct tcttcgcgtc gttgatctgg
      421 gcgccgggca gggcttctag gcgcgcgatc tcgtccagag gcaggtcggt gaacagcttc
      481 aggaagcggc ccacgtcggc gtcctccgtg ttgcgccaga actgccagta atcgtagggg
      541 ctcagctgct cggcgttcag ccacacggcg cccgacatgg tcttgcccat cttggcgccc
      601 gaggcggtgg ccagcagcgg cgtcgtcagg ccgaatgcgg acttctggtc gacacgccgg
      661 gtcagctcca cgccattcag gatattgccc cactggtccg agccgcccat ctgcagcacg
      721 cagccgaact tgcggttcag ctccaggaag tccaccgcct gcatcagcat gtagttgaat
      781 tcgaggaagg tcatcggctg ctcgcgctcg agtcgcagct tgaccgagtc gaaggccagc
      841 atccggttga ccgtgaagtg cacgccgtag tcgcgcagga actgcacgta gccaaacttc
      901 gacagccact cgtcgttgtc gaccatgaca gcgtcggtcg gcccgtcgcc aaaggtcagg
      961 aacctggcga acacctgctt gatgccggcg atgttggcgg cgatgtcggc gtccgacagc
     1021 agcttgcggc tctcgtcctt gccggtggcg tcgaaaccga tataggcggt gacgatcccg
     1081 ccagcggcgg ccgcgtcgag ttcatccggg tgggtgatct ggtggatata gccgcgcgcc
     1141 tgcagggtct gcaggaattc cgacttgaac gaggcgggcg cggacatgga tcgactctct
     1201 ggatgggacg actcgtgcgg agacggtggg ccgtctagca cgggcgggcc gggatgtggc
     1261 gagggtttca tagggtcagt ctccggatag gggccggagc gctatgatct tgtgagacgc
     1321 gacccggcac ggcgggggcg tatcgacgtg tcaggccggc ctcccgctat ttgagcgggc
     1381 ggtaataggc gcaggagcgg aagcgagcgt cgatcatggg gcctagctac aggccccgcg
     1441 ccatggcagt caacggcgat gcgcatattg ggattcatga cgggaacctc gctcgacgcc
     1501 gtcgatatgg cggtgctgga gaccgacggc gaagacatcc aggcgtttgg gccggcgggc
     1561 g
//
LOCUS       DAOMEO010000860         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8142_length_1561_cov_2.117530, whole genome shotgun sequence.
ACCESSION   DAOMEO010000860 DAOMEO010000000
VERSION     DAOMEO010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_1561_cov_2.117530"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..416)
                     /locus_tag="PLH31_16230"
     CDS             complement(132..416)
                     /locus_tag="PLH31_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HQR90776.1"
                     /translation="MIKSELIARLANENPHLTQKDVERVVSVILERMIGALEGGGRVE
                     LRGFGALSVRSRPARSGRNPRTGETVDVRAKHVPFFKSGKELRARLNADE"
     gene            complement(836..>1561)
                     /locus_tag="PLH31_16235"
     CDS             complement(836..>1561)
                     /locus_tag="PLH31_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQR90777.1"
                     /translation="KIVSTSQEVDVVVLDVDPSKRRVSLGLKQALANPWDSFLETHPV
                     GSTVEGEVKNATEFGLFIGLDNDIDGMVHLSDIDWSASGEEAMARYKKGDMVKAKVLD
                     VDVEKERISLGIKQLGGDPMSGDTYRKNQTVTVTVTEVTTGGIEVRFGEDDALMTAFI
                     RKSDLSRDRQEQRPERFAVGDRVDAQITNIDKAARRVSMSIKSLEMAEEKEAIEQFGS
                     SDSGASLGDILGAALRERATPKE"
BASE COUNT          277 a          485 c          497 g          302 t
ORIGIN      
        1 atcgatgaca ttgccccgcg agatgaactc gcgaaattcc ttgatgacgc tcatgaccgg
       61 tctcgccgat tagccctgcg gaagtcgcga agctgatggc gttcggcccc caaggtcaag
      121 ggcgggtcag gttactcgtc ggcgttgagc cgggcccgca gttccttgcc gctcttgaag
      181 aacggcacgt gcttggcgcg aacatcgacg gtttcgccgg tgcgcggatt gcggcccgag
      241 cgcgctgggc gcgagcggac cgacagggcg ccaaagcccc gaagctcgac gcgaccgccg
      301 ccttccagag cgccaatcat gcgctccagg attacgctga caacgcgctc gacgtctttc
      361 tgcgtcaggt gcggattttc attcgcgagc ctggcgatga gttcagactt gatcatcgat
      421 atccccggcg ccggagggag gaactggcgc gaccggacct ttgcccaagg gtggttgagt
      481 cttcaagagc ctgcgtgcga ttctcatcca gcttgagagt ttacctcgca tggccatggc
      541 acatgaacgt cgcgcctgag gccctgtgag aggcgccagc gggcgtcaga tggcgcccgg
      601 agagcatttt tttgggcgat aaccgtcgag tgaggtcgaa agaaccacac ggcgccgagg
      661 tgcgccgagc gcgcctattt tgagcgggcg cgccccgcca tggtgagtgg cgtcttcacc
      721 aaatgcgtcc ccatccaaag acttagcggg accgcaagca aggcggacgc aaagaaaacg
      781 gcggccggat cgctccggcc gccgctgact tcagtctctc aggtgagagc gaagactatt
      841 ccttcggggt ggcccgttcg cggagagccg cgcccaggat atcgcccagc gaagcgccgg
      901 agtccgacga gccgaactgc tcgatggctt ccttttcttc ggccatttcc agcgacttga
      961 tcgacatcga aacgcggcgg gccgccttgt cgatattggt gatctgagcg tcgacgcggt
     1021 cacccacggc gaagcgctcg gggcgctgtt cctgacggtc gcgcgacagg tccgacttgc
     1081 ggatgaacgc ggtcatcagg gcgtcgtctt cgccgaagcg aacttcgatg ccgccggtgg
     1141 tcacttcggt cacggtgacg gtcacggtct ggttcttgcg ataggtgtcg cccgacatcg
     1201 gatcgccacc cagctgcttg atgcccagcg agatgcgttc cttctcgacg tcaacgtcga
     1261 gaaccttcgc cttgaccatg tcgcccttct tgtagcgggc catggcttct tcgcccgagg
     1321 cgctccagtc gatgtccgac aggtgcacca tgccgtcgat gtcgttgtcg aggccgatga
     1381 acagaccaaa ctcggtcgcg ttcttgactt cgccttcgac ggtcgagccg accgggtggg
     1441 tttcgaggaa cgaatcccac gggttggcca gagcctgctt caggcccagc gatacgcgac
     1501 gcttggacgg atcgacgtcc agcacgacca cgtcgacttc ctgcgaggtc gagacgatct
     1561 t
//
LOCUS       DAOMEO010000861         1559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8149_length_1559_cov_2.096410, whole genome shotgun sequence.
ACCESSION   DAOMEO010000861 DAOMEO010000000
VERSION     DAOMEO010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_1559_cov_2.096410"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLH31_16240"
     CDS             complement(<1..168)
                     /locus_tag="PLH31_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HQR90778.1"
                     /translation="MGEELVLTQARQWLGTPYRHQASLRGVGCDCLGLIRGIWQALYG
                     DEPEPPPPYRPD"
     gene            complement(282..926)
                     /locus_tag="PLH31_16245"
     CDS             complement(282..926)
                     /locus_tag="PLH31_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HQR90779.1"
                     /translation="MRAVPAELAARLEEGAASLCHAWILTRSDAVVLGFTDHDRDLVV
                     DGATCRAASGWTAGAHETAAGFAPGLSAALGALDDGAVTVEDIEAGLYDGASVACLSV
                     DWSRPDLFVRLWTARIARIRREGEAFTAELEGPLADLDRVAGRTFGRLCDAVLGDARC
                     GVSPAPGAVCDKRWATCVGTFANGVNFRGFPAMPGEDFLTLYPSEGERHDGGRR"
     gene            complement(926..1558)
                     /locus_tag="PLH31_16250"
     CDS             complement(926..1558)
                     /locus_tag="PLH31_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HQR90780.1"
                     /translation="MSAFHDIRLPARLAFGCTGGVERRTQVVTLASGYERRSSPWVQG
                     RRRYLIATATRPLDEAAELVAFFEARRGRLHGFRFRDPSDFKSCLPSAQPAATDQAIG
                     TGDGARKIFQLAKTYGTGAETAVRTIAKPVAGTVKVAVAGVALAPAAFTVDLATGLVT
                     LVTAPAAGAAVRAGFEFDTPVRFDIDRLDVQMEGFAAARITACPLVEVRA"
BASE COUNT          231 a          576 c          564 g          188 t
ORIGIN      
        1 gtcggggcgg tagggcggcg ggggttcggg ctcgtcgccg tagagggcct gccagatgcc
       61 ccggatcagc cctaggcagt cgcagccgac accccgcagg ctggcctggt ggcggtaggg
      121 cgtgccgagc cattgacggg cttgggtgag aaccagctcc tcccccactg ggggaggtgg
      181 cccagagggc cggagggggc agcgcggcgt tcgcccagat ggccccctca gtcgctccgc
      241 gacagctccc ccagaggggg agcagctctc cgcgcccgcc ctcaccgcct acccccgtca
      301 tgccgttcgc cctcggaggg atacagcgtc agaaagtcct cgcccggcat ggccgggaag
      361 ccccggaagt tgacgccatt ggcgaatgtg ccgacgcagg tcgcccagcg cttgtcgcaa
      421 accgcgcccg gcgcaggcga aaccccgcag cgcgcatcac cgagcacggc gtcgcacagc
      481 cggccaaagg tgcggcccgc cacacggtcg agatccgcca gcggcccttc cagctcggca
      541 gtgaaggcct cgccctcgcg gcggatgcgg gcgatgcgcg ccgtccacag gcgcacgaac
      601 aggtccggcc ggctccagtc tacgctcagg cacgccaccg acgccccgtc atagagcccg
      661 gcctcgatgt cctcgacggt caccgcgccg tcgtccaggg ccccgagcgc ggccgacagc
      721 cccggtgcga aaccggccgc cgtctcgtgg gcgccggcgg tccagccgct ggcggcccgg
      781 caggtcgcgc catcgaccac caggtcgcgg tcgtggtcgg tgaagcccag aaccacggca
      841 tcgctgcggg tcaggatcca ggcgtggcac aggctggccg cgccctcctc aaggcgtgcg
      901 gccagttcgg cgggaacggc gcgcatcagg cccgtacctc gaccagcgga caggcggtaa
      961 tccgcgccgc cgcaaagcct tccatctgca cgtccagccg atcgatatcg aagcgcaccg
     1021 gggtgtcgaa ctcgaacccc gccctgaccg ccgcgccggc ggcgggcgcc gtgaccaggg
     1081 tgacgaggcc ggtcgccaga tccaccgtga aggcggccgg ggccagggcg acgcccgcca
     1141 ccgcgacctt gactgtgccg gccacgggtt tggcgatggt gcggaccgcc gtttcagcgc
     1201 cggtcccgta ggtcttggcc agctggaaga tcttgcgggc gccgtcaccg gttccgatcg
     1261 cctggtccgt cgccgccggc tgggccgagg gcaggcacga cttgaagtcg ctgggatcgc
     1321 gaaagcgaaa gccgtgcagc cgcccgcgcc gggcctcgaa gaaggcgacc agctcggcgg
     1381 cctcgtccag cggccgggtc gcggtggcga tcaggtagcg gcggcggccc tgcacccacg
     1441 gactggagcg gcgctcatag cccgaggcca gggtcaccac ctgcgtgcgc cgctcgacgc
     1501 cgccggtgca gccgaaggcc agccgcgcag gcaggcggat gtcgtggaag gcgctcatg
//
LOCUS       DAOMEO010000862         1558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8152_length_1558_cov_2.431803, whole genome shotgun sequence.
ACCESSION   DAOMEO010000862 DAOMEO010000000
VERSION     DAOMEO010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8152_length_1558_cov_2.431803"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLH31_16255"
     CDS             complement(<1..736)
                     /locus_tag="PLH31_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90781.1"
                     /translation="MSDLFQAAGLTPHAPTPLAERLRPQTLGEVVGQEHLLGPEGPIG
                     RMAAAQRLASMILWGPPGTGKTTIARLLAKAGGYEFQQISAVFSGVADLKKAFEQARG
                     RRLAGQSTLLFVDEIHRFNRAQQDGFLPFVEEGVITLVGATTENPSFELNGALLSRCQ
                     VFVLKRLDEEALEALLAKAEAVEGRKLPLDSEARFTLIAMADGDGRYLLTLTETLLSI
                     GTDTPLNAVQLGQFLQKRRPAYDKNRE"
     gene            complement(831..>1558)
                     /locus_tag="PLH31_16260"
     CDS             complement(831..>1558)
                     /locus_tag="PLH31_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQR90782.1"
                     /translation="INTFIISRSGSSSGVGFAIPAAVVKQVVNAALGGGHSVVRPWLG
                     VKGQPVTAEIARSLGMRAPQGVLVAQVYPGSSADKAGLQESDVILSIDGKAVNDEGGG
                     AFAIGTHKVGDRVPMVIRRGDNERTLTVRAETAPESPPRDQKTLTGRNPFDGATVLNL
                     SPAVAQDLNVDPFVGKGVLVSKIGQGFALNAGLRPGDFIREVNGRTINTVAELVAVSN
                     GGNRAWTVTIERGGQRISARFNT"
BASE COUNT          246 a          524 c          547 g          241 t
ORIGIN      
        1 cctcgcggtt cttgtcgtag gcgggccggc gcttctgcag gaattggccc agctggacgg
       61 cattcagcgg cgtgtcggtg ccgatcgaca gcagggtctc ggtcagggtc agcagatagc
      121 ggccgtcgcc gtcggccatg gcgatcaggg tgaagcgggc ctcggagtcc agcggcagct
      181 tgcgaccttc gaccgcctcg gccttggcca gcagggcctc aagcgcctcc tcgtccaggc
      241 gcttgagcac gaacacctgg cagcgggaca agagagcgcc gttcagctcg aacgacgggt
      301 tctcggtggt ggcgccgacc agggtgatca ccccctcctc cacgaacggc aggaaaccgt
      361 cctgctgggc gcggttgaag cggtggatct cgtcgacgaa cagcagggtg ctctggccgg
      421 ccaggcggcg gccgcgcgcc tgctcgaagg ccttcttcag gtcggcgacg ccggagaaca
      481 ccgccgagat ctgctggaac tcgtagccgc cggccttggc cagcagccgg gcgatggtgg
      541 tcttgccggt gccgggcggg ccccacagga tcatcgaggc cagacgctgg gccgccgcca
      601 tccggccaat ggggccttcg ggacccagca gatgctcctg ccccaccacc tcgcctagcg
      661 tctggggccg caggcgctcg gcgagcggag tcggcgcgtg gggcgtcagg ccggcggctt
      721 ggaaaagatc agacatggct ctggtctaca aaactgcggg gaccctgacc aacgaagaag
      781 acccggcgcg gggtccgcgc cgggtccatt ggcttggctc aatcaaatga tcaggtgttg
      841 aaccgcgccg agatgcgctg accgccccgc tcgatggtga cggtccaggc gcggttgccg
      901 ccgttcgaca cggcgaccag ttcggccacc gtattgatcg tgcggccatt gacctcacgg
      961 atgaagtcgc ccggacgcag gccggcattg agcgcaaagc cctggccgat cttgctgacc
     1021 agcacaccct tgccgacaaa cgggtcgaca ttcaggtcct gggcgacggc gggcgacagg
     1081 ttcagcaccg tggcgccatc gaacgggttg cggccggtca gggtcttctg gtcgcggggc
     1141 ggactttcag gcgcggtttc agcgcgaacc gtcagggtgc gctcgttgtc gccgcgacgg
     1201 atcaccatcg gcacgcgatc gcccaccttg tgggtgccga tggcgaaggc gccgccgccc
     1261 tcgtcgttca cggccttgcc gtcgatggac aggatcacgt cgctttcctg cagccccgcc
     1321 ttgtcggccg acgagccggg atagacctgg gccaccagca caccctgcgg ggcgcgcatg
     1381 cccaggctgc gggcgatctc ggcggtgacg ggctggccct tcacgcccag ccacggacgc
     1441 accacgctat gaccgccgcc cagggcggcg ttgaccacct gcttgaccac ggcggcggga
     1501 atggcgaagc ccaccccgct cgacgagccg gaccgcgaga tgatgaaggt gttgatgc
//
LOCUS       DAOMEO010000863         1552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8198_length_1552_cov_2.894456, whole genome shotgun sequence.
ACCESSION   DAOMEO010000863 DAOMEO010000000
VERSION     DAOMEO010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8198_length_1552_cov_2.894456"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1222
                     /gene="plsX"
                     /locus_tag="PLH31_16265"
     CDS             137..1222
                     /gene="plsX"
                     /locus_tag="PLH31_16265"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQR90783.1"
                     /translation="MSKNIVISIDAMGGDHGPSVVVPGVAKAAQMLPGVRFLLHGDEI
                     RLNAELAKAPAARAVSEVRHTDKHIAMDEKPAQAMRRGKGSSLWNAVESLREGEAQAC
                     VSAGNTGALMAISKLILRMGADLERPAIVASWPTMKGITAVLDVGANVESDAAQLVEF
                     AIMGAAFHHAVHGSMRPTVGLLNVGSEDQKGHEEVREAHAILKETQLDFDYRGFVEGN
                     DIAYGTVDVVVTDGFTGNVALKTAEGLARFFSNEIKSTLTSGPLAMLGALIASGALKQ
                     MRERLDPGRVNGGPLLGLNGIVVKSHGGADAKGFASALRVAADLARSDFTAEIDRNLK
                     RLTAPAAPGGASDGAASACVERDGASE"
     gene            1219..>1552
                     /locus_tag="PLH31_16270"
     CDS             1219..>1552
                     /locus_tag="PLH31_16270"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516805.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HQR90784.1"
                     /translation="MSVIRSVVTGVGAYLPPLIVTNNDLAKFVDTSDEWIAERTGIRQ
                     RHQAAEDQAVSDLAVEAARSALQAAGKTAMDVDLIIVATTTPDLTFPATATIVQRKLG
                     APVGIAFDI"
BASE COUNT          284 a          486 c          517 g          265 t
ORIGIN      
        1 ctctgccgac agcggaaatc tcgcccttcg ccgcgctcaa ggcgctcaaa cccaagggcg
       61 attcggacta aacgggtccg actcgccgcg cttgcgtgat actcatgcag gcgcctcagg
      121 agctcaaagc gccaaagtgt ccaagaacat agtcatttcg atcgacgcca tgggcggcga
      181 tcacggcccg tcagtcgttg tgccgggcgt ggccaaggcc gcccaaatgc tgccgggtgt
      241 gcgctttctg cttcatggcg acgaaatcag gctgaatgcc gagcttgcga aggctccggc
      301 ggccagggcg gtaagcgagg tccggcacac cgacaagcat atcgccatgg acgagaagcc
      361 cgcccaggcg atgcgcaggg gcaagggctc aagtctctgg aacgctgtgg agtcgctacg
      421 ggagggtgag gcgcaagctt gcgtttcggc cggcaatacc ggggccctaa tggccatctc
      481 caagctgatc ctgcgcatgg gcgcggatct ggagcgtccc gccatcgtcg ccagctggcc
      541 gaccatgaag ggcatcacgg cggttctcga tgtcggcgcc aatgtcgaga gtgacgcagc
      601 ccaactggtc gaatttgcga tcatgggcgc ggcctttcac cacgcggtgc acggctccat
      661 gcgcccgacg gtcggcctgc tcaacgtcgg ttcggaagac caaaagggcc acgaagaggt
      721 gcgtgaggcg cacgctattc tcaaggaaac ccagctcgat ttcgactatc gcggcttcgt
      781 cgagggcaat gacattgcct atggcactgt tgatgttgtc gttacggacg gtttcaccgg
      841 caatgtggcg ttgaagaccg ccgaggggct ggcgaggttt ttctccaacg agatcaagtc
      901 gaccctgacc tccggaccct tggccatgct gggcgccttg atcgcgtcgg gcgcgctgaa
      961 gcagatgcgt gagcggctcg acccgggcag ggtcaatggc gggccgctct tgggtttgaa
     1021 tggtatcgtg gtcaaaagtc acggcggcgc ggacgccaag ggcttcgcct cggcgttacg
     1081 ggtcgccgca gatctggcgc ggagcgactt cacggccgaa atagatcgaa atctgaagcg
     1141 cctgaccgct ccggcggccc cgggcggcgc cagcgacggg gctgcctccg cctgcgtcga
     1201 gcgcgatgga gcaagcgagt gagcgtaatc cgcagcgtgg tcacgggtgt cggcgcctat
     1261 ctgccgccgc tgatcgtcac caataatgac ctggccaagt tcgtcgacac cagcgatgag
     1321 tggatcgccg agcgtacggg catccgtcag cgccaccagg cggccgagga ccaggctgtg
     1381 tcggatctgg ccgtggaggc cgccaggtcg gcgcttcagg ccgccggcaa aaccgccatg
     1441 gacgttgacc tgatcatcgt cgcgaccacg acgcccgacc tgaccttccc ggcgacggcc
     1501 accatcgtgc agcgcaagct gggcgcgccg gttggcatcg ccttcgacat cc
//
LOCUS       DAOMEO010000864         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8204_length_1551_cov_3.127005, whole genome shotgun sequence.
ACCESSION   DAOMEO010000864 DAOMEO010000000
VERSION     DAOMEO010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_1551_cov_3.127005"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLH31_16275"
     CDS             complement(<1..434)
                     /locus_tag="PLH31_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HQR90785.1"
                     /translation="MSDDLKIEGHASLFWTRDLNDDVTAAGAFAESLAETGAAGVKML
                     HQHDDAEPIGVWDEVVEDARGLFVRGRILRSTPRGRLVAALVEAGALDGLSIGFRQVK
                     ARQDGSLRVLKRVELWEVSVVTFPMLPGARLRLSESRSPPEA"
     gene            complement(431..676)
                     /locus_tag="PLH31_16280"
     CDS             complement(431..676)
                     /locus_tag="PLH31_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90786.1"
                     /translation="MTTPNRWRLDRQISVALIVAVALQAATALMWAGRASARIDEMHK
                     RLDTQAPVAERLARLEEQAVATRAALARIESRLERDR"
     gene            complement(820..>1551)
                     /locus_tag="PLH31_16285"
     CDS             complement(820..>1551)
                     /locus_tag="PLH31_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HQR90787.1"
                     /translation="RGWPLAYDYQAGGRTVRIGRDADGWLPVLHLKLFNPTSDHYGLS
                     PLEAAAFAIDVHNASSAWNKALLDNSARPSGALVYSNREAGDRLSEEQFDRLKAELAG
                     AHSGAGNAGRPLLLEGGLDWRAMSLTPAEMDFTDGKHAAAREIALAFGVPPQLLGVPG
                     DATYANYREANAAFWRHTVAPLVERAARAMTAWLEVKFPGVRIAPDLDAVPAFSAERD
                     ALWARLDAASFLTPEERRRLAGLDG"
BASE COUNT          220 a          542 c          572 g          217 t
ORIGIN      
        1 cacgcctccg gtggagagcg actttcagac agtcgaagcc gagccccagg caacatcgga
       61 aaagtaacca ccgacacctc ccaaagctcc acccgcttga gtacccgcaa gctcccgtcc
      121 tgacgcgcct tgacctggcg aaagccgatg gagaggccgt ccagcgcgcc ggcctccacg
      181 agcgccgcga ccagccggcc gcgcggggtc gagcgcagga tgcggccacg gacgaacagg
      241 ccgcgcgcgt cctcgacgac ctcgtcccag acgccgatcg gctcggcgtc gtcgtgctgg
      301 tgcagcatct tgacccccgc cgcgccggtc tcggccaggc tttcggcgaa ggcgccggcg
      361 gcggtgacgt cgtcgttgag gtcccgggtc cagaacagcg aggcgtggcc ctcgatcttc
      421 agatcatcac tcatcggtcg cgctccagcc gggattcgat gcgggccagc gcggcgcggg
      481 tggcgacggc ctgctcttcc agccgggcca ggcgctcggc gacgggggcc tgggtgtcga
      541 ggcgcttgtg catctcgtcg atccgggccg aggcgcgccc ggcccacatc agggcggtcg
      601 cggcctgcag ggcgacggcc acgatcaagg cgaccgagat ctggcggtcc agccgccagc
      661 ggttgggggt ggtcatgttg ggtgtctctt caaagtcctc cccctgaagg gggaggggtc
      721 ggcggagccg acggaggggg aagttggccg gcgtgggatg acttccccct ccggccctgc
      781 gggccgcctc ccccttcagg gggaggactt gtcgcggagt cagccgtcca gccccgccaa
      841 ccttctccgc tcctcgggcg tgaggaaact ggccgcgtcc agccgcgccc acagggcgtc
      901 gcgttcggcg gagaaggcgg gcacggcgtc gaggtcgggg gcgatgcgga cgcctgggaa
      961 cttgacctcc agccaggccg tcatggcgcg cgccgcccgc tcgaccagcg gcgcgaccgt
     1021 gtggcgccag aaggcggcgt tggcctcgcg atagttggcg taggtggcgt cgccgggcac
     1081 ccccagcagc tggggcggca cgccaaaggc cagggcgatc tcgcgggcgg cggcgtgctt
     1141 gccgtcggtg aagtccatct cggctggggt cagcgacatg gcccgccagt cgaggccgcc
     1201 ttccagcagc agcggccggc cggcgttccc cgccccggaa tgggccccgg ccaactcggc
     1261 cttcaggcgg tcgaactgct cttccgagag ccggtcgcca gcctcgcggt ttgaatagac
     1321 cagggcgccg gacggccggg ccgagttgtc cagcagcgcc ttgttccagg cgctggaggc
     1381 gttgtgcaca tcgatggcga aggccgccgc ctccaggggc gagaggccgt aatggtcgct
     1441 ggtggggttg aacagcttca ggtgcaacac cggcagccag ccgtcggcgt cgcgcccgat
     1501 gcgcaccgtg cggccgccgg cctggtagtc ataggccagc ggccagccgc g
//
LOCUS       DAOMEO010000865         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8215_length_1549_cov_2.047523, whole genome shotgun sequence.
ACCESSION   DAOMEO010000865 DAOMEO010000000
VERSION     DAOMEO010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_1549_cov_2.047523"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="pseB"
                     /locus_tag="PLH31_16290"
     CDS             <1..814
                     /gene="pseB"
                     /locus_tag="PLH31_16290"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HQR90788.1"
                     /translation="GDVRDRERLTLALRGVDIVIHAAALKQVPAAEYNPSECIHTNVL
                     GAENVVWASLTNGVKKVVALSTDKACNPINLYGATKLASDKTFVAANNLSGDIGTRFA
                     VVRYGNVVGSRGSVVPLFKRLLANGATELPITDERMTRFWITLNEGVNFVLSSLELMR
                     GGEIFVPKIPSMTMPDLVKAMSPTAGVKVIGIRPGEKLHEMMISADDARATVELEDRY
                     VIEPTFVEYPRQRFGPEDGAKPAEEGFCYSSDNNDDWLSAPGLLAMLEEKAG"
     gene            814..>1549
                     /locus_tag="PLH31_16295"
     CDS             814..>1549
                     /locus_tag="PLH31_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR90789.1"
                     /translation="MAQPPFLPYGRQTIEDDDVAAVAAALRADFLTTGPTVEAFETAF
                     KEKVGAQHAIAVSNGTATLHLAMLALGVGEGDVCIAPSITFLATANCARYVGAEVVFA
                     DVDPHSGLMTPDTLADALARAGDRRVKAILPVHLRGDVCDLPAMRGMASAAGAVLVED
                     APHALGSVATFGGVKHNVGDGAYSAFASFSFHPVKTLATGEGGMLTTNDEGLAARARS
                     LRGHGMVRPPGSDPWWYEMPELGFNYR"
BASE COUNT          249 a          529 c          514 g          257 t
ORIGIN      
        1 gggtgacgtg cgcgaccgcg agcgcctgac cctggccctg cgcggggttg atatcgtcat
       61 tcacgccgcc gccctgaaac aggtgccggc cgccgaatat aatccgtccg agtgcatcca
      121 caccaatgtg ctgggggccg agaacgtggt ctgggccagc ctgaccaatg gcgtgaagaa
      181 ggtcgtcgcc ctgtcgaccg acaaggcctg caacccgatc aacctgtatg gcgcgaccaa
      241 gctggcctcg gacaagacct tcgtggccgc caacaacctg tcgggcgaca tcggcacccg
      301 ctttgcggtg gtgcgctacg gcaatgtggt gggctcgcgc ggcagcgtcg tgccgctgtt
      361 caagcgcctg ctggccaatg gcgccaccga gctgccgatc accgatgagc gcatgacccg
      421 gttctggatc accctgaacg agggcgtgaa ctttgtgctg tcgtcgctgg agctgatgcg
      481 cggcggcgag atcttcgtgc cgaaaattcc gtccatgacc atgcccgacc tggtcaaggc
      541 catgtcgccg accgccggcg tcaaggtcat cggcatccgt ccgggcgaaa agctgcacga
      601 gatgatgatc agcgccgacg acgctcgggc caccgtcgag ctggaagacc gctatgtgat
      661 cgagccgacc ttcgtcgaat atccccgcca gcgctttggt cccgaggacg gcgccaagcc
      721 ggccgaggaa ggcttctgct acagcagcga caacaatgac gactggctgt cggcccccgg
      781 cctgctggcc atgctggagg agaaggcggg ctgatggccc agcctccttt ccttccctac
      841 ggtcgccaga ccatcgagga cgacgatgtc gccgccgtcg ccgccgccct gcgcgccgat
      901 ttcctgacca ctggcccgac ggtcgaggcc tttgagaccg ccttcaagga aaaggtcggg
      961 gcgcagcacg ccatcgccgt ctccaacggc accgccaccc tgcacctggc catgctggcc
     1021 ctgggggtgg gtgaaggcga tgtctgcatc gcgccgtcga tcaccttcct ggcgaccgcc
     1081 aactgcgccc gctatgtggg ggccgaggtg gtgttcgccg atgtcgatcc ccattccggc
     1141 ctgatgaccc ccgacaccct ggccgacgcc ctggcgcgag ccggcgaccg tcgggtgaag
     1201 gccatcctgc ccgtgcacct gcgcggcgat gtctgcgacc tgccggccat gcgcggcatg
     1261 gccagcgcgg ccggcgcggt gctggtcgag gacgcgcccc acgccctggg ttcggtcgct
     1321 acgtttggcg gcgtgaagca caatgtcggc gacggggcct attcggcctt cgccagtttc
     1381 tcgttccatc cggtcaagac gctcgccaca ggcgagggcg gcatgctgac caccaatgac
     1441 gagggtctgg cggcgcgggc acggtccctg cgaggccacg gcatggtgcg gccgccgggg
     1501 tccgatccct ggtggtacga gatgccggag ctgggcttca actatcgca
//
LOCUS       DAOMEO010000866         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8247_length_1544_cov_2.526528, whole genome shotgun sequence.
ACCESSION   DAOMEO010000866 DAOMEO010000000
VERSION     DAOMEO010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8247_length_1544_cov_2.526528"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1544
                     /locus_tag="PLH31_16300"
     CDS             <1..>1544
                     /locus_tag="PLH31_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HQR90790.1"
                     /translation="VNGKPSLSIETAGLTLVGFENGWSSALGQPYTITYGFRATAPET
                     MPDDSAGFSRFNTAQIRQAELALTSWSDVANITFVRVGSGAVGEAAYSNQASILFSNY
                     SSGVDGASAFAYLPGNPAFSASSGDVWINATLGYNVTPTANNYGGSVLVHELGHAIGL
                     GHPSDYDVVDGASFTYAEHAEYYQDSRQYTVMSYFSERNTGASYEGRYASAPQLDDIA
                     AAQAEYGANMTTRTSDTTYGFNSNAGRSWFEISTASTKVVFAVWDAGGADTFDFSGYS
                     NAQTIDLREGHFSDVGGLVGNVAVAAGAKIEDARGGSGADTIHGNDLANWLRGEAGAD
                     AINGLAGDDRLDGNDGNDTITGGDGFDRINGNIGADFGYGGAGADWVVGGQNNDQLFG
                     EAGDDIVNGNLGNDVCYGGVGSDIVRGGQGDDVLSGDEGDDWLSGDRGADNITGGLGA
                     DIFYSFAGAGLDLITDFNRAQGDRIQLDPGETYSVYQSGANAIIDLGGGDQIILAGVN
                     ISALTG"
BASE COUNT          294 a          478 c          492 g          280 t
ORIGIN      
        1 gtcaatggca agcccagcct gtcgatcgag acggcgggcc tgaccctggt gggtttcgag
       61 aacggctggt cttccgcctt gggtcagccc tacaccatca cctatgggtt tcgcgcgacg
      121 gcgcccgaga ccatgcccga tgattcggct ggcttttcac ggttcaacac cgcccagatc
      181 agacaggcgg agctggccct gacgtcttgg tcggatgtcg ccaacatcac ctttgtccgg
      241 gtcggttccg gcgccgttgg agaggcagcc tacagcaacc aggcctccat cctgttcagc
      301 aactattcca gcggcgtcga tggcgcatca gcgtttgcct atctcccggg caatcccgcc
      361 ttctcggcca gttccggcga cgtctggatc aacgcaacac tgggctataa tgttacgccg
      421 acagccaaca actacggcgg ctcggtcctt gtccatgaac tgggccacgc catcgggctg
      481 ggccatccct ccgactatga cgtcgtcgac ggcgccagct tcacctatgc ggaacatgct
      541 gaatattatc aggattcccg ccaatatacg gtgatgagct atttcagcga gcgcaataca
      601 ggcgccagct atgagggacg ctacgcctcg gcgccgcagc tcgacgacat agcggccgct
      661 caggcggaat acggggccaa tatgacgacc cggaccagcg acaccaccta cggattcaat
      721 tccaacgccg gacgatcctg gttcgagatc agcaccgcct cgaccaaggt ggtctttgct
      781 gtctgggatg ctggcggagc cgacacgttc gacttttctg gctacagcaa cgcccagacg
      841 atcgatctgc gagaaggcca tttctcggac gtcggtggtc tggtcggcaa tgtggctgtg
      901 gccgctggcg ccaagatcga agacgcccgg ggcggctcgg gcgcagacac catccacggc
      961 aacgacctgg ccaattggct acgcggcgaa gctggcgccg acgccataaa tggtctggcg
     1021 ggcgatgatc ggcttgatgg caatgacggc aatgacacca tcacgggcgg cgacgggttc
     1081 gatcggatca acggcaatat cggcgctgat ttcggctatg gcggcgcggg tgcggactgg
     1141 gtcgtgggag gtcagaacaa cgaccagctg tttggcgagg cgggtgatga catcgtcaac
     1201 ggcaaccttg gcaatgacgt ctgctatggc ggcgtgggca gcgacatcgt tcgcggcggt
     1261 cagggcgacg atgtcctgtc cggcgacgaa ggcgatgact ggctgtcggg cgaccgcggc
     1321 gccgacaaca tcaccggcgg gctcggggca gacatcttct acagcttcgc cggcgccggt
     1381 ctggacctga tcaccgactt caaccgcgcg cagggcgacc ggatccagct ggatccgggc
     1441 gagacctaca gtgtctacca gagcggggcg aacgcgatca tcgacctggg cggcggtgac
     1501 cagatcatcc tggcgggcgt gaatatctcg gccttgaccg gcga
//
LOCUS       DAOMEO010000867         1543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8256_length_1543_cov_1.932796, whole genome shotgun sequence.
ACCESSION   DAOMEO010000867 DAOMEO010000000
VERSION     DAOMEO010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8256_length_1543_cov_1.932796"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="uppS"
                     /locus_tag="PLH31_16305"
     CDS             <1..428
                     /gene="uppS"
                     /locus_tag="PLH31_16305"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQR90791.1"
                     /translation="IGLSWVYLAYGQVPWIAGGNTKFTLQVAFNYGGRADIADAARAF
                     AEAVERGEAKAADLDEAAFERLLSTSTAPAPDLIVRTSGERRISNFLLWECAYAELVF
                     QDVLWPDYGPEALSAAIAEYRGRDRRYGGIAADDVAVAG"
     gene            406..1254
                     /locus_tag="PLH31_16310"
     CDS             406..1254
                     /locus_tag="PLH31_16310"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQR90792.1"
                     /translation="MTSPSPAKRFNWKNLRVRVLSAAVLVPTVVSAVWFGGVWFLALV
                     AVCVALLAREWGMMSAPRAPRGVALAIGAAALVAVVAAFQGHYYLMWPLLAAGALLAA
                     LIARGAVERRVDAAYGVIYIAPACIALVWLRELDAGLSWTLLLFAVTWFADIFAFVVG
                     SILKGPKLWPKFSPNKTWSGFFGGLVAASVAAVGIDALSGLAPALPDMNLTWPVAAAI
                     GFLGGLSTMMGDLWESMLKRRFGVKDSGDLIPGHGGLLDRVDGLMFAAIVIAGVRLIY
                     LYGWAH"
     gene            1242..>1543
                     /locus_tag="PLH31_16315"
     CDS             1242..>1543
                     /locus_tag="PLH31_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQR90793.1"
                     /translation="MGTLTVIRKVVVLGSTGSIGVSTLGLFEAAGVEVEILALTAGRN
                     VERLIEQSRRWRPAVAVIEDERCLDALRAGLKDTGVRAAAGTSAVIEAAAMGADWV"
BASE COUNT          209 a          469 c          538 g          327 t
ORIGIN      
        1 tgatcggact ttcttgggtt taccttgcct atggacaagt accctggatt gcaggaggca
       61 atacgaagtt tacgctccag gtggctttca attatggtgg ccgggccgat attgccgacg
      121 ccgcccgcgc cttcgccgag gcggttgagc gcggcgaggc caaggcggcc gatctcgatg
      181 aagcggcctt cgaacgcctt ctatccacct ccaccgcacc ggcgcctgac ctgatcgtgc
      241 gcaccagcgg agagcggcgg atctcgaatt tcctgctgtg ggaatgcgcc tacgccgagc
      301 tcgtcttcca ggacgtgctc tggcctgact acggacccga agcgctctcg gcggccatcg
      361 cagaataccg cgggcgtgac cgacgctatg gaggcattgc cgccgatgac gtcgccgtcg
      421 ccggctaagc gcttcaattg gaaaaatcta cgagtacggg tgctttcagc cgccgtgctc
      481 gtacccacag tcgtatccgc agtctggttt ggcggcgtct ggttcctcgc gcttgtcgcg
      541 gtctgtgtcg ccttgctggc gcgcgagtgg ggaatgatga gcgcgccgcg cgcgcccagg
      601 ggcgtggcgc tggcgattgg cgccgccgct ctcgtggccg tcgtggccgc tttccagggc
      661 cactattacc tgatgtggcc gctcctggcc gccggggcgc ttctggcggc cctgatcgcc
      721 cgcggcgccg tcgagcgcag agtggatgcg gcctatggcg tgatctatat tgcgccggcc
      781 tgcatcgccc ttgtgtggct gagggagctg gatgcgggcc tgagctggac gcttctgctg
      841 tttgcggtca cctggttcgc cgacatcttc gcctttgtgg tcggcagcat cctcaagggg
      901 cccaagctgt ggcccaagtt ctcacccaac aagacctggt ctggcttctt tggcggcctg
      961 gtggcggcct cggtcgccgc tgtcgggatt gatgcgctgt cgggcctggc gccggcgctt
     1021 ccagacatga atctgacctg gcccgtagcc gctgcgatcg gcttcctggg cggcctgtcg
     1081 accatgatgg gcgatctctg ggaatcgatg ttgaagcggc ggttcggcgt gaaagattcc
     1141 ggcgacctga tacccgggca tggtgggctg ctcgatcgtg tggacggcct gatgttcgcg
     1201 gcgatcgtca tcgccggtgt gcgcctgatt tacctctacg gatgggcaca ttgacggtta
     1261 tccggaaggt cgtggttctc ggctctacag ggtcaatcgg cgtttcgacc ctggggctgt
     1321 tcgaggccgc cggtgtcgag gtcgagattc tggcgctgac cgctggtcgc aatgtcgagc
     1381 gcctgatcga gcagtcgcgt cgctggcgcc cggccgtggc cgtgattgaa gacgagcgct
     1441 gtctcgacgc cctgcgcgcg ggtctgaagg acaccggggt ccgggcggcg gcgggaacct
     1501 cggcggtcat cgaggctgcg gcgatgggcg cagattgggt gat
//
LOCUS       DAOMEO010000868         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8261_length_1541_cov_2.782638, whole genome shotgun sequence.
ACCESSION   DAOMEO010000868 DAOMEO010000000
VERSION     DAOMEO010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_1541_cov_2.782638"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLH31_16320"
     CDS             complement(<1..295)
                     /locus_tag="PLH31_16320"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HQR90794.1"
                     /translation="MPPKNPTAPPPAPDTTVGLETLTGPGGELHQQSADQAHQLTTNQ
                     GVPISDNQNSLKAGARGPVLLQDFILREKIMHFDHERIPERVVHARGSAAHGFF"
     gene            complement(346..882)
                     /locus_tag="PLH31_16325"
     CDS             complement(346..882)
                     /locus_tag="PLH31_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQR90795.1"
                     /translation="MATTANTKAKNSTRRAATKAAAAPKAVGPDAISLLEADHREVDD
                     YFEQYETLTDDAAKKALADKICLALKVHAQIEEELLYPPAREETGDGHLIDEAIVEHM
                     GAKTLIAQIEASAPGQPLYDARVKVLGEQVRHHVEEEEQELFPEVRATGLDLDALGAK
                     LTARKAELMALLAPAPAA"
     assembly_gap    1221..1230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1245..>1541
                     /locus_tag="PLH31_16330"
     CDS             1245..>1541
                     /locus_tag="PLH31_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR90796.1"
                     /translation="MGSPRLEQDASLRPERILDLSERLSTVAGEKIGEIARVNRAAKM
                     LSINALIVAARAGEAGKGFAIVAEEFKKISTEIDAVAAALESQVRADLDELTAIG"
BASE COUNT          231 a          459 c          544 g          297 t
ORIGIN      
        1 cgaagaagcc atgggcggcc gaaccccggg catgaaccac ccgctcgggg atgcgctcgt
       61 gatcgaagtg catgatcttt tcgcgcagga tgaagtcctg cagcagcacc ggaccgcggg
      121 cgccggcctt cagcgagttc tgattatcgc tgatcgggac gccctggttg gtcgtcagct
      181 gatgggcctg gtcagcgctc tgctggtgca gttcgccgcc aggtccggtc agggtttcga
      241 ggccgacggt ggtgtcgggt gcgggcggcg gggctgtggg atttttgggg ggcatgagct
      301 gtcctggccc gcttgggcta ttgcgagaaa agagaggggg tggcgtcagg cggccggggc
      361 gggcgccagc agcgccatca gttcggcctt gcgcgccgtg agcttggcgc ccagagcgtc
      421 gagatccagg ccggtcgcgc gcacctcggg aaagagctcc tgctcttctt cttcgacatg
      481 gtggcggacc tgctcgccca gcaccttgac cctggcgtca tagagcggct ggcctggcgc
      541 gctggcttcg atctgggcga tcagggtctt ggcccccatg tgctcgacga tggcttcgtc
      601 gatgaggtgg ccatctccgg tctcttcgcg agccggcgga tagagaagtt cctcttcgat
      661 ctgggcatga accttgaggg ccaggcagat cttgtcggcg agcgccttct tggcggcatc
      721 gtccgtgagg gtttcatact gttcgaagta atcgtcgact tcgcgatggt cggcctcgag
      781 cagcgaaatg gcgtctggac cgacggcctt gggggcggcg gcggccttgg tggccgcgcg
      841 gcgggtgctg ttcttggctt tggtgtttgc cgtggtggcc atgagagctc tcgattgtga
      901 tgaggctcaa tcaacagacg ggtccgatga cctgttccgg cgcgacgggg cgcctcggcg
      961 gcgcctggcg cgcgggggcc gccagaagcc aatggatcca gcgcttcagt cgcaacaggc
     1021 ggtgtcgggt aaaatcggcg cgctcgccgt tccggccgcc gtcgacggca tcaaatgata
     1081 ctcgggcctg gccggagcaa gggcggcggt cggcggagct gggctgtccc aacctctctg
     1141 ctcgttcctc gcgcccaccc caatgccgtc attttgagcg aagagcgctc aatatcttcg
     1201 cgggcttgag acctcctttt nnnnnnnnnn cgttcagtag tcgcgtgggg tcgcctaggt
     1261 tggaacaaga cgccagtctg cgtccggaac gtattctgga cctttccgaa cgcctcagca
     1321 cggtcgccgg cgaaaagatc ggcgaaatcg cgcgggtcaa tcgcgcggcc aagatgctgt
     1381 cgatcaacgc cctgatcgtc gccgcccggg cgggcgaggc cggcaaggga ttcgccatcg
     1441 tcgccgagga gttcaagaag atctccaccg agatcgacgc ggtcgccgcc gccctggaaa
     1501 gccaggttcg cgccgatctg gacgagctga ccgcgatcgg c
//
LOCUS       DAOMEO010000869         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8266_length_1541_cov_2.045760, whole genome shotgun sequence.
ACCESSION   DAOMEO010000869 DAOMEO010000000
VERSION     DAOMEO010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_1541_cov_2.045760"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLH31_16335"
     CDS             <1..665
                     /locus_tag="PLH31_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90797.1"
                     /translation="GDELQYARFAPILAERGATVRLVCRPQVSRLFRRSLPGVEVYEA
                     SGRVEIPDCDFYVMGPDIVGLMDYTLETVPGAPYLKAAETVASSARIGLVAAGSAAHG
                     NDAYRSLNAADGLTLAAALGPTIGLDYAATGGKDFADTADIIEGLDVVVTVDTAIAHL
                     AGGMGKTVWLLLSRIDTDWRWGRGRERSTWYPSAEFFFSDAKGQWGETIERLGARAKA
                     LG"
     gene            complement(765..>1541)
                     /locus_tag="PLH31_16340"
     CDS             complement(765..>1541)
                     /locus_tag="PLH31_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90798.1"
                     /translation="SATPAALSRVIGNLIDNALKFTQQGGVTVKISGDDHGLQIAVID
                     TGIGIAAKDHESIFERFVQVDNSSTRKVGGIGLGLAICREISGRMGGSLTVRSALGIG
                     ARFDFDAPIPSVVSSEAPISDEVDASAATQPLSLLVVDDNPVNRRILASLVEPFGVTC
                     GFAVSGREAVAAWRDQPWDAIFMDVHMPDMDGVEATRAIRREEALRGGARTPIIAVTA
                     SVLSHEVESYIEAGMNGVLPKPIEAAALASTLSFCAAAAD"
BASE COUNT          252 a          487 c          534 g          268 t
ORIGIN      
        1 tcggcgacga gctgcagtat gcccggttcg cgccaatcct ggccgaacgc ggtgcgaccg
       61 tcaggctggt ctgcaggccg caggtgtcgc ggctgtttcg ccgatcattg ccgggcgtcg
      121 aggtttatga ggccagcggc cgggtcgaga tccccgactg cgacttctac gtcatggggc
      181 ccgatatcgt cggcctgatg gactacacgc tcgagaccgt gccgggagcg ccctatctca
      241 aggccgccga gacggtggcg tcttccgcgc ggatcggcct ggtcgccgcg ggcagcgccg
      301 cgcatggcaa cgacgcctat cgctcgctca atgcggcgga cgggttgacg ctggccgccg
      361 cgctgggtcc caccataggt ttggactacg ccgccaccgg cggtaaggac ttcgccgata
      421 ccgctgatat cattgaaggt ctcgatgttg tcgtgacggt tgatacggcc atcgcccacc
      481 tggccggggg gatgggcaag accgtgtggc tgctgctgag ccggatcgac accgactggc
      541 gatggggccg ggggcgggaa cgctcgacct ggtacccttc cgcggagttc tttttcagcg
      601 acgccaaggg gcagtggggc gaaaccatcg agcgtctggg cgcgcgcgcc aaggctctgg
      661 gttgagagct tcagcgccag gattggctcg ggcgtgacgc cgaaacttct gatttggagc
      721 ctttttcgcc cggccctgat gaagtcatca ggtcggaaag ggcgctagtc cgcagcggct
      781 gcgcagaatg acagggtgga ggccagggcc gcagcttcga tgggcttggg caggacgcca
      841 ttcatccccg cctcgatgta gctttccacc tcatgcgaga gcacgctggc cgtgacggcg
      901 atgatggggg ttcgggcgcc gccacgcagg gcctcttcac gacggatggc gcgtgtggcc
      961 tccacgccgt ccatgtccgg catgtggaca tccatgaaaa tcgcgtccca gggctggtcg
     1021 cgccaggccg cgacggcttc gcggccactg acggcgaagc cgcaggtgac gccgaagggc
     1081 tcgaccagcg aggcgagaat gcgccggttg accggattgt cgtccaccac cagcagcgag
     1141 aggggctggg tggcggcgga cgcgtcgacc tcgtcgctga tcggggcttc ggaagataca
     1201 accgacggga tcggcgcgtc gaaatcgaac cgcgcgccga tgcccagggc cgatctgacc
     1261 gtcagtgagc cgcccatccg cccggagatc tcgcgacaga tcgccaggcc cagaccaatc
     1321 ccgccaacct tgcgggtcga tgaattgtcg acctgaacga aacgctcgaa gatcgactcg
     1381 tgatccttgg cggcgatgcc gatcccggtg tcgatgacgg cgatctgaag tccgtgatca
     1441 tcgccgctga tcttgaccgt cacgccgccc tgctgggtga atttcagcgc gttgtcgatc
     1501 agattgccga tcacccggct cagcgccgcc ggcgtggcgc t
//
LOCUS       DAOMEO010000870         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8279_length_1540_cov_2.719192, whole genome shotgun sequence.
ACCESSION   DAOMEO010000870 DAOMEO010000000
VERSION     DAOMEO010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8279_length_1540_cov_2.719192"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLH31_16345"
     CDS             complement(<1..478)
                     /locus_tag="PLH31_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQR90799.1"
                     /translation="MNAPSKPNDYSAYPDAEGRFGGFGGRYVAETLMPLVLDLGKAYA
                     QAKADPKFQDQLKSFNTHYAGRPSPMYFAERLTEHFGGAKIYFKRDELNHTGSHKINN
                     ALGQILLAMRMGKTRIIAETGAGQHGVATATVCARFGLPCVVYMGATDVERQKPNVF"
     gene            complement(508..1164)
                     /locus_tag="PLH31_16350"
     CDS             complement(508..1164)
                     /locus_tag="PLH31_16350"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQR90800.1"
                     /translation="MSSPFKTSAKICGLSTPESVRAAVEGGAAYLGFVFFDKSPRNLT
                     PEAASRLVDPVRGGPVKTVAVTVDPDDALIDRLMATMKPDLIQVHGRETPARVRQIAE
                     RSGAGVIKAFSVSSPSDVDQASAFETVAEQFLFDAKPVEGSALPGGTGARFDWTLLEG
                     RRFSRPHFLAGGLDPWNVADAIRLSGAPLMDVSSGVERGPGLKDPALITAFLDAVKRA
                     "
     gene            complement(1219..>1540)
                     /locus_tag="PLH31_16355"
     CDS             complement(1219..>1540)
                     /locus_tag="PLH31_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90801.1"
                     /translation="AIKGHTPGHVGYEISSGGQTLLDIGDTAHSEIVSLARPDWVIQF
                     DRDAPVATASRIALLKTLSANGGLVFSPHFPFPGVGKVEAAGEGFVWKAGLPVQDAHA
                     GHAH"
BASE COUNT          241 a          478 c          565 g          256 t
ORIGIN      
        1 ggaacacgtt gggcttttga cgctcgacgt cggtggcgcc catatagacc acgcacggca
       61 ggccgaagcg ggcgcagacg gtggcggtgg ccacgccgtg ctggccggcg ccggtctcgg
      121 cgatgatccg ggtcttgccc atgcgcatgg ccagcaggat ctggcccagg gcgttgttga
      181 tcttgtgcga gccggtgtgg ttcagctcgt cgcgcttgaa atagatcttg gcgccgccga
      241 aatgctcggt cagccgctcg gcgaaataca tcgggctggg gcggccggca taatgggtgt
      301 tgaagctctt cagctggtcc tggaacttcg ggtcggcctt ggcctgcgca taggccttgc
      361 ccaggtcgag cacgagcggc atcagggtct cggccacata gcgaccgcca aagccgccga
      421 agcggccctc cgcgtcggga taggccgaat agtcattggg cttgctggga gcgttcacga
      481 tcgtcttccg ggctgggctt gtcagcttta ggcgcgtttg acggcgtcga ggaacgccgt
      541 gatcagagcc gggtccttta ggccgggacc gcgctccacg ccagaggaaa cgtccatgag
      601 cggggcgccc gatagccgga tggcgtcggc gacgttccac ggatccagcc ctcccgccag
      661 gaagtggggg cgagaaaatc gcctgccttc cagcagggtc cagtcgaacc gtgcgccggt
      721 tccgccgggg agggcagagc cttccacggg cttggcgtcg aacaggaact gctcggcgac
      781 ggtctcgaag gcgctggcct ggtcgacatc cgagggcgat gaaaccgaga acgccttgat
      841 caccccggcg ccggaccgct cggcgatctg gcgcactcgg gcgggggttt ccctgccatg
      901 cacctggatc aggtcaggct tcatcgtcgc catcaggcgg tcgatcaggg cgtcgtcggg
      961 atcgacggtg acggccaccg ttttcacggg tccgccccgt accggatcca ccagccggct
     1021 ggcggcttca ggggtcaggt tgcgagggct tttgtcgaag aacacgaagc ccagataggc
     1081 cgcgccgcct tctacagcgg cgcgcaccga ctcgggcgtc gaaagcccgc agatcttggc
     1141 gctggtcttg aagggcgaac tcatgggcgg gaggtacggc gcgggcgccc cggtgacaag
     1201 ggcccgcgcc ggatcggcct agtgcgcgtg gccggcgtgg gcgtcctgga cagggaggcc
     1261 ggccttccag acgaagcctt cgcccgccgc ctcgaccttg cctacgcccg ggaacgggaa
     1321 gtggggcgag aagaccaggc caccgttggc gctcagggtc ttgagcaggg cgatgcggct
     1381 ggcggtggcg acgggggcgt cgcggtcaaa ctggatcacc cagtcggggc gggccaggga
     1441 gacgatctcg ctgtgggccg tatcgccgat gtccagcagg gtctggccgc cggagctgat
     1501 ctcgtagcca acatggccgg gcgtatggcc cttgatggcc
//
LOCUS       DAOMEO010000871         1540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8280_length_1540_cov_2.692256, whole genome shotgun sequence.
ACCESSION   DAOMEO010000871 DAOMEO010000000
VERSION     DAOMEO010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8280_length_1540_cov_2.692256"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLH31_16360"
     CDS             <1..327
                     /locus_tag="PLH31_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR90802.1"
                     /translation="VYLHDTPSRARFASYSRLASHGCVRVEKPVDLARAVMEGDATWT
                     PQVIDDTIASGKTVRAQLPQQIAVFLLYWTAYVTPDGQVNFRDDPYGWDKLLVQRIAA
                     SLPGSA"
     gene            382..999
                     /locus_tag="PLH31_16365"
     CDS             382..999
                     /locus_tag="PLH31_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HQR90803.1"
                     /translation="MIARRRLFQLGFSALGAAAIGPSAHAQDDIIGVLLEAQPPAPVT
                     TVAVAQPALEAAPKWLSLHNLHTAEKLQAVYWEKGAYVPDALDALNKVLRDHRTGEVA
                     PMNVQLFDLLADIHHKTETRTAFQIISGYRSPASNRLLSARSGEVAKRSLHMDGKAMD
                     IYLEDVALDRLRAAALDLKRGGVGYYPDSKFVHVDVGPFRRWQGS"
     gene            1024..1371
                     /locus_tag="PLH31_16370"
     CDS             1024..1371
                     /locus_tag="PLH31_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90804.1"
                     /translation="MSAERRSNPFGWIFLGFVVGVIATFGAILFLTAGLGGYDEGSDE
                     MRTAADDAAAEAMVTAPLAPAARKVEAPVATVPPPAVDVPVRQTPQVDSQMADDAAAA
                     GMTSRATPDQATN"
BASE COUNT          258 a          503 c          484 g          295 t
ORIGIN      
        1 gtctatctgc acgacacgcc cagccgcgcg cgcttcgcca gctatagccg tctggccagc
       61 catggctgcg tgcgtgtcga aaagcccgtc gatctggccc gcgccgtgat ggaaggggac
      121 gctacctgga ccccccaggt gattgacgac accatcgcca gcggcaagac cgtccgagcc
      181 cagctgccgc agcagatcgc ggtcttcctg ctgtattgga cggcatatgt gacacccgat
      241 ggccaggtca atttccgcga tgacccctat ggctgggaca agctcctggt gcagcggatc
      301 gccgcatccc tccctggctc tgcttgacct tcgggctgcc ttggggtgtc ctgccggcag
      361 ttcggtgccg gggatagcgt tatgatcgca cgacgtaggc tttttcagct gggtttcagc
      421 gcgctaggcg cggctgcgat tggtccgtcg gctcatgcgc aggacgatat cattggcgtg
      481 cttcttgagg cacagccccc ggcgcccgtc accacggttg ccgtggcgca acccgccctc
      541 gaggccgccc ccaaatggct ctccctgcac aatcttcaca cggccgaaaa gttgcaggcc
      601 gtgtactggg aaaagggcgc ctacgttccc gacgcccttg acgccctgaa caaggtcttg
      661 cgtgatcatc gcaccggcga ggtggctccg atgaacgtcc agttgttcga tctgctggcg
      721 gacatccacc acaagaccga aacaagaacg gcattccaga tcatttccgg ctatcgctca
      781 cccgccagca accgccttct gtcggcccgt agtggcgagg tcgccaagcg cagcctgcac
      841 atggacggca aggccatgga catctatctg gaagacgtgg ccctcgaccg gcttcgcgct
      901 gcggcgctgg atctgaaacg gggcggcgtc ggctactatc ccgacagcaa atttgtacac
      961 gtcgacgtcg ggccgttccg acgctggcag ggaagctagg ccctcagggg cagggagtcg
     1021 ggaatgagcg ctgagcgtcg tagcaatccg ttcggatgga tcttcctggg ctttgtcgtg
     1081 ggcgtcatcg cgacgttcgg cgccattctg ttcctgaccg ccggcttggg ggggtatgac
     1141 gagggctccg acgaaatgcg caccgccgcc gatgacgcgg ccgctgaagc catggtcacg
     1201 gcgcccttgg cgccggccgc tcgcaaggtt gaagcgccgg tcgcgactgt tccaccgcca
     1261 gccgttgatg tgccggttcg ccagacgccg caggtcgact cgcagatggc cgacgacgcc
     1321 gcagctgctg gcatgacctc gcgcgcaacg ccggatcagg cgaccaacta gaagtttgga
     1381 tcaggccgct tcgctttcca gcggctgggt gttcagggcg ccgtgacgcg ccatgcgcat
     1441 cagggcctcc aactgctgct ccagatcctc gatccgttcg gcttgtttga tgacaagtcg
     1501 ttcgaggtcc tgcaggcgtt cttcgacacg ttcagccatg
//
LOCUS       DAOMEO010000872         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8302_length_1538_cov_3.407283, whole genome shotgun sequence.
ACCESSION   DAOMEO010000872 DAOMEO010000000
VERSION     DAOMEO010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_1538_cov_3.407283"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLH31_16375"
     CDS             <1..651
                     /locus_tag="PLH31_16375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR90805.1"
                     /translation="EGKATRLLGATVNITDRKRAEMHLRLLVNELNHRVKNSLATIQA
                     IAAQSFNASRPLPQAQEAFTSRIVALSEAHDLLTRENWEGAELHDIAARLSVLHGGPT
                     RFELKGPAIRLSPRAALSLSMALHELATNAVKYGALSVPDGRIRIVWDLAPGGGAQRL
                     DLTWTESGGPPVKPPTQRGFGSRLIERGLAAELSGEASIKFEPAGVVCHIRALLDA"
     gene            complement(648..>1538)
                     /locus_tag="PLH31_16380"
     CDS             complement(648..>1538)
                     /locus_tag="PLH31_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90806.1"
                     /translation="AREAMDGRLTPPPWASLPLRAMLGEPLPLLGMMAVGAGLFWLAT
                     TALGRRFSDAAAAAQGAASKRPRGAVKPAGAFAAGAFKATVRKELRLVGRDATLLSQV
                     LLRVLYLIPLALVLARSAAKVDAIALAGGAGIVAFMAGQVAGSLAWITLSAEDTPDLL
                     AVSPTSTTTLRRAKLVAALIPVALLLVLPIAALTWFQPLTGLITAVGAGLAALSCGLI
                     NVWHQKPGKRADFNRRRGASWLATLAEIMIGGLIAGATTLTVMGLAGWAAPLLIVALT
                     VLMVMRRTDEQIGRALRLAG"
BASE COUNT          240 a          579 c          533 g          186 t
ORIGIN      
        1 gagggcaagg ccacccgcct gctgggcgcc acggtcaaca tcaccgaccg caaacgggcc
       61 gagatgcacc tgcgcctgct ggtcaacgag ctgaaccacc gggtgaagaa ctcgctggcc
      121 acgatccagg ccatagcggc ccagagcttc aacgcctcgc gccccctgcc ccaagcccag
      181 gaggccttta ccagccgcat cgtcgccctg tccgaggccc atgatctgct gacccgcgaa
      241 aactgggagg gcgccgagct gcacgacatc gccgcgcgcc tgtcggtgct gcacggcggt
      301 ccgacgcgat tcgagctgaa ggggcctgcc atccggctgt cgccgcgcgc ggccctgtcg
      361 ctctcgatgg ccctgcatga actggccacc aatgcggtca agtacggcgc gctgtcggtt
      421 cccgacggcc gcatccgcat tgtctgggac ctggcgcccg gcggcggcgc ccagcggctg
      481 gacctgacct ggaccgagag cggcggcccg cccgtcaagc cgcccaccca gcgcggcttc
      541 ggctcccgcc tgatcgagcg cggcctggcc gccgaactgt cgggcgaggc ctccatcaag
      601 ttcgaaccgg ccggcgtggt ctgtcacatc cgggcgctgc tggacgccta acccgccagt
      661 ctcaacgccc tgccgatctg ctcgtcggtt cgccgcatca ccatcagcac cgtcagggcc
      721 acgatcagca gcggcgcggc ccagccggcc agacccatca ccgtcagggt ggtggcgccg
      781 gcgatcaggc cgccgatcat gatctcggcc agggtggcca gccacgaggc gccgcgacgg
      841 cggttaaagt cggcccgctt gcccggcttc tgatgccaga cattgatcag cccgcaggac
      901 agggcggcca ggcccgcgcc gacggcggtg atcaggccgg tcaggggctg gaaccaggtc
      961 agggccgcga tcggcaggac cagcaacagg gccaccggga tcagggccgc gaccagcttg
     1021 gcccggcgca gggtggtggt cgaggtcggc gacacggcca gcaggtccgg ggtgtcctcg
     1081 gccgacaggg tgatccaggc cagacttccg gccacctgtc cggccatgaa ggcgacgatg
     1141 cccgccccgc cagccagggc aatggcgtcc accttggcgg ccgagcgggc cagcaccagg
     1201 gccagaggga tcagatacag cacccgcagc agcacctgcg acagcagggt ggcgtcgcgt
     1261 cccaccagcc gcagctcctt gcgcaccgtg gccttgaagg ccccggccgc gaaggcgccg
     1321 gcgggtttca ccgcgccgcg cggacgtttc gacgccgccc cctgcgccgc cgcagcggcg
     1381 tcggagaacc ggcgtcccag ggccgtggtc gccagccaga acaggcccgc gcccacggcc
     1441 atcatgccca gcagcggcag gggctcgccg agcatggcgc gcagcggcag gctggcccag
     1501 ggcggcgggg tcaggcgacc gtccatcgcc tcgcgggc
//
LOCUS       DAOMEO010000873         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8304_length_1538_cov_2.692515, whole genome shotgun sequence.
ACCESSION   DAOMEO010000873 DAOMEO010000000
VERSION     DAOMEO010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_1538_cov_2.692515"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLH31_16385"
     CDS             <1..847
                     /locus_tag="PLH31_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HQR90807.1"
                     /translation="ERAGNAALEEVVMAMQVRGDKLPYETKIDTTHITRASRYVSAIT
                     GFPVQYNKAIVGKNAFAHESGIHQDGMLKNRETYEIMTPESVGQAPSSLVMGKHSGSH
                     AFRAKLKDLGYELGQNALKEAFGRFKDLADRKKHVYDDDIIALVDDALARGSEKIRVM
                     HLRVVAGTEGQSAELTLDVDGQERTAEATGDGPVDAVFNAIHKIVPHSAALRLFQVHA
                     VTEGTDAQAQVSVRLEEDGRIATGQAADTDTLTASAKAYVNALNNLFARKEKSRPEAA
                     IASGF"
     gene            912..>1538
                     /locus_tag="PLH31_16390"
     CDS             912..>1538
                     /locus_tag="PLH31_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HQR90808.1"
                     /translation="MLWIPITIAAAALQVARNAAQRSVMGGAGPWGATLVRFLFGLPF
                     ATVFAAVAWAATPGATAHATTWFWLACAAGAGLQIAATAALLTAMQRSTFALGTAMQQ
                     SGLPFALVWGALLFGDHVGPLAWAGGLVASAGLAALSWPRGEVVMRPGAALAGLGSGA
                     IFALSANCFRQASLAFDPGHPVASAFVTVMVVQAIQTVALTSVLAVRNP"
BASE COUNT          250 a          556 c          507 g          225 t
ORIGIN      
        1 cgagcgcgcc ggcaatgccg cgctggaaga agtcgtgatg gccatgcagg ttcgcggcga
       61 caagctgccc tacgagacca agatcgacac cacccacatc acccgcgcca gccgctacgt
      121 ctcggccatc accggtttcc cggtgcagta taacaaggcc atcgtcggca agaacgcctt
      181 cgcgcatgaa agcggcatcc accaggacgg gatgctgaag aaccgcgaga cctacgagat
      241 catgaccccg gaatcggtcg gccaggcgcc gtccagcctg gtcatgggca agcactcggg
      301 cagccacgcc ttccgcgcca agctgaaaga cctgggctac gaactgggcc agaacgccct
      361 gaaggaggcc ttcggccgct tcaaggacct ggccgaccgc aagaagcacg tctacgacga
      421 cgacatcatc gccctggtcg acgacgccct ggcgcgcggc tcggaaaaga tccgcgtgat
      481 gcacctgcgc gtcgtggccg gcaccgaagg ccagtcggcc gagctcaccc tggacgtcga
      541 cggccaggaa cgcaccgccg aggccacggg cgacggtccg gtggacgccg tgttcaacgc
      601 catccacaag attgtgccgc acagcgccgc tctgcgcctg ttccaggtgc acgcggtgac
      661 cgaaggcacc gacgcccagg cccaggtctc ggtgcgtctt gaagaggatg gccgcatcgc
      721 caccggacag gcggccgaca ccgacacgct taccgcctcc gccaaggcct atgtgaacgc
      781 gctcaacaac ctcttcgccc gcaaggaaaa gagccgcccc gaagcggcga tcgccagcgg
      841 cttctaggcg cgtctctcag ccatcgccca gcgacccctt cttccgataa ggaagaaggg
      901 aaggacgccg catgctctgg attcccatca cgatcgccgc cgccgccttg caggtcgccc
      961 gcaacgccgc ccagcgctcg gtgatgggtg gagcggggcc atggggcgcg accctggtga
     1021 ggttcctgtt cggcctgccg ttcgcgaccg tgttcgccgc ggtcgcctgg gcggcgacgc
     1081 cgggcgcgac ggctcacgcc accacctggt tctggctggc ctgcgcggcc ggagcggggc
     1141 tgcagatcgc cgcgacggcc gcgctgctca ccgccatgca acgctcgacc ttcgccctgg
     1201 gcaccgccat gcagcagagc ggcctgccgt tcgccctggt ctggggcgcg ctgttgttcg
     1261 gcgaccacgt cggcccgctg gcctgggccg gcggactggt ggcgtcggcc ggcctcgcgg
     1321 cgctgtcctg gccgaggggc gaggtcgtga tgcggccggg cgcggctctg gccggtctgg
     1381 ggtccggcgc gatcttcgcg ctcagcgcca attgtttccg ccaggccagc ctggcctttg
     1441 atcccggtca ccctgtcgcc tcggccttcg tcacggtgat ggtggtgcag gccatccaga
     1501 ccgtggccct gaccagcgtc ctggccgtgc gaaatccc
//
LOCUS       DAOMEO010000874         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8305_length_1538_cov_2.443695, whole genome shotgun sequence.
ACCESSION   DAOMEO010000874 DAOMEO010000000
VERSION     DAOMEO010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8305_length_1538_cov_2.443695"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="PLH31_16395"
     CDS             <1..113
                     /locus_tag="PLH31_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR90809.1"
                     /translation="RADTLQRYALAVFLAPAIMLMSVCRPLLWKITGRPI"
     gene            complement(159..542)
                     /locus_tag="PLH31_16400"
     CDS             complement(159..542)
                     /locus_tag="PLH31_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90810.1"
                     /translation="MTRSLFTLGLIAGAALALVACAATPPPVDIAARCDVTVKFGSYG
                     MGIDRPLAERVAIAVKADRDIARVERKPWGREGEYDLCLEARTGRDARAIYERYRATL
                     PGRALQAPTSIEGPDGLRYETIAPM"
     gene            complement(539..1438)
                     /locus_tag="PLH31_16405"
     CDS             complement(539..1438)
                     /locus_tag="PLH31_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQR90811.1"
                     /translation="MSRFIEIVEVGPRDGLQNEKLALSVEEKLDLIRQLEAAGARRME
                     VVSFVNPNRVPQMAGAEEIMAALEPDLKHSRIGLVLNMRGWERCVAAQCDEANVVVCA
                     TDGFGTRNQGSTTSQQIDALAGIVERQAVEGGPPITATLSVAFGCPFDGEVSEDQVVA
                     IIRDIALLGVPEIAIADTIGVADPWTVRKRIEAVRAAAPDARVRMHFHDTRNTGLANA
                     FASVEAGVDVLDASVGGLGGCPFAPNATGNIGTEDLVYMLERAGFETGYDLGALIEVG
                     RTISERLGKAPASSLARAGGFPA"
BASE COUNT          246 a          521 c          527 g          244 t
ORIGIN      
        1 agcgcgccga caccctgcag cgctacgccc tggccgtgtt cctggccccg gccatcatgc
       61 tgatgtcggt ctgccggccg ctgctgtgga agatcaccgg gcggccgatc taggctgcca
      121 gctggatccc ggctttcggc gggctagtgg ctacaccgct acatcggcgc gatcgtctcg
      181 tacctcaaac catccggacc ctcgatcgag gtcggcgcct gcaaggcccg gcccggcagc
      241 gtggcgcgat agcgttcgta gatcgcgcgg gcgtcgcggc cggtccgggc ctcaaggcac
      301 agatcatatt cgccctcgcg gccccagggc ttgcgctcga cccgggcgat gtcgcgatcg
      361 gccttgacgg cgatggcgac gcgctcggcc agagggcggt cgatgcccat gccatagctg
      421 ccgaacttga cggtaacgtc gcagcgcgcg gcgatatcga ccggcggcgg ggtggcggcg
      481 caggcgacca aggcgagggc cgcgccggcg atcaggccaa gggtaaagag ggagcgggtc
      541 atgcggggaa acccccagcg cgcgccagtg aggaggccgg cgccttgccc aggcgctcgc
      601 tgatcgtgcg cccgacctcg atcaacgcgc caagatcata gccagtctca aaaccggccc
      661 gctccagcat gtagaccaga tcctcggtgc cgatattgcc ggtggcgttg ggcgcgaagg
      721 gacaaccgcc caggccgccg accgaggcgt ccagcacgtc gaccccggcc tcgacgctgg
      781 caaaggcgtt ggccaagccc gtattgcggg tgtcatggaa gtgcatgcgc acgcgcgcgt
      841 ccggcgcggc agcgcgcacc gcttcgatcc gcttacggac ggtccaggga tcggcgacac
      901 cgatggtgtc ggcgatggcg atctcgggaa cccccaagag ggcgatgtcg cgaatgatgg
      961 ccacgacctg gtcttcggaa acctcgccat cgaagggaca gccgaaggcc accgacaggg
     1021 tcgcggtgat cggcgggccg ccctcgaccg cctgacgctc gacgataccc gccagcgcat
     1081 cgatctgctg gctggtggtc gagccctggt tgcgggtccc gaaaccgtcg gtggcgcaga
     1141 ccacgacatt ggcctcgtcg cactgtgccg ccacgcagcg ctcccagccg cgcatgttca
     1201 gcaccaggcc gatgcgggag tgtttcaggt ctggctcaag cgccgccatg atctcttcgg
     1261 cgccagccat ctggggcaca cggtttggat tgacgaacga caccacctcc atgcggcggg
     1321 cgccggcggc ctcaagctgg cggatcaggt ccagcttttc ctcgaccgac agggccagtt
     1381 tttcgttctg aagaccgtcg cgcggcccga cctcgacgat ctcgatgaag cggctcacgg
     1441 gcgatcctcg atcaggggct gggtgtcggg ttcgatctct gtctggggct cgccgcgata
     1501 gatcgtcagg cgctcgtcgt cgccgtcgga atagttct
//
LOCUS       DAOMEO010000875         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8316_length_1537_cov_2.379892, whole genome shotgun sequence.
ACCESSION   DAOMEO010000875 DAOMEO010000000
VERSION     DAOMEO010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8316_length_1537_cov_2.379892"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1537
                     /locus_tag="PLH31_16410"
                     /pseudo
     CDS             337..1537
                     /locus_tag="PLH31_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518627.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
BASE COUNT          249 a          548 c          505 g          235 t
ORIGIN      
        1 gcgcctcccc aacccactgg cagccgattt cggcgcgagg tcgagacgcc gcgtcctttt
       61 caacccacaa aaccttgcct tcctcgcccc tgtggcgagg acccatcggt ccgctgtctc
      121 agcgcatctc gatcacgctc aacgccggcg gaacgctggg tcctgggcac agggtccagg
      181 aaggcagggg tttggttggc gctgggtgat tgaagagggg gcgtcccggt gctggccagg
      241 tccagaatga caccggtgga caaaactcgt ccagaccggc attttcgcgc ctcgaaaccg
      301 gcgctcaaga cgccagctct accggggatg aaaccgatgc gccgccacct tcaacgctcc
      361 gctggcctcg ccgccctcgc cctcaccctc agcgcctgcg ccaccgccgc cagcgtggcc
      421 ggaccaaggg cggtcctcgc cttccccggc gccgagggcg cgggccggct cagcgtcggc
      481 gggcgcggcg gggcggtgct caaggtcacc aatctcaacg acgccggccc tggctccctg
      541 cgcgccgcca tcgaggccaa gggcccgcgc accatcgtgt tcgaggtggc cgggaccatc
      601 gcgctgcaaa gcccgctgaa gattgccaat ggccagatca ccctcgccgg ccagaccgcg
      661 ccgggcggcg ggatcaccct gcgcgaccag accctggtgg tggccgccga cgatgttgtg
      721 atccgcttca tccgctcgcg tctgggcgcc gagagcagga ccgagggcga cgcgatctgg
      781 atcgccaagg gcgccggatc attctggacc acgtctcggc cagctggtcg gtggacgaga
      841 ccctgtcggc ctcggcccgc ttcaccccgc cggaacaggg cttctatgac ctgaccgttc
      901 agtggtcgat catcagcgaa agcctggcca ggtcgatcca cgccaagggc gaccatggct
      961 atggcagcct gatccggggc ggccagggtt ccaggataag ctttcaccac aatctgtggg
     1021 ccaatcacgt ggcgcgcatg ccgcgcccgg gcaattatga cggacccgac aaggacccgg
     1081 tcggcgcctt catcgagttc cgctccaacg tcttctacaa ctggggcggc ggccattccg
     1141 gctacgacgc cgacaaggcg gccatggtcg cctacaactt tgtcgacaac gcctatgtga
     1201 tgggtcctga cagccagcag gcggtcgcct tcaaggaaag caacagcctg gccagggcct
     1261 ggttcgctgg caacagcatg aacggcgtgg ttccagccga cccgtggagc ctggtgaccg
     1321 gcctgcaggc ctcgggctat cgccaggtcg cgccgatcga tatcgcgccc gtggccgccg
     1381 agcccgccgc cagcgcctat gagcgtgtgc tggccgaggc cggcgcctcg gtccgtcgcg
     1441 atgccgtcga cgcccgggtc gtggcgggcg tgcgcaaccg gacgggcagg atcatcaaca
     1501 ccgagcagga tgtcggcggc tggccagtcc tgccgcc
//
LOCUS       DAOMEO010000876         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8321_length_1537_cov_1.628880, whole genome shotgun sequence.
ACCESSION   DAOMEO010000876 DAOMEO010000000
VERSION     DAOMEO010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8321_length_1537_cov_1.628880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLH31_16415"
     CDS             complement(<1..918)
                     /locus_tag="PLH31_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQR90812.1"
                     /translation="MLRILRLGAPGFEDAGAGPDWRLPDDAVWIELVDPSRTEEVSVE
                     QSIGLLLPTREEMAEIEASSRLYQEDGGTFMTATILVNADGDLPTAAPVTFVLIGQRL
                     ITIRYVEPRAFSVFAAQAERQPSLCPGGPQTFLGLLDAIIDRTADIIERTASEVEAQS
                     RAIFSRPRGDAFEQILNRLGRAQNINSKARDSLVSLARLLSFASLADQFEGDRELRDH
                     LKSLQRDVQSITDHSSYVSSNITFLLDAALGLINIEQNAIFKIFSVFSAVFLPPTLIA
                     GVYGMNFEHMPELRWMQGYPMALGLMLAAG"
     gene            complement(986..>1537)
                     /locus_tag="PLH31_16420"
     CDS             complement(986..>1537)
                     /locus_tag="PLH31_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HQR90813.1"
                     /translation="DARTWWGQAAGKYDTGSAPKIGSVLSFKPTGRMNLGHVAFVSQV
                     LTDRVIQVTHANWSVMNGTRGQVERDVTVVDVSPNGDWSQVKVWYDPIRDLGTTVYPT
                     HGFIYQNAQATTIAAATSKLALAQNAAVSLAKSAANQVAASVRASPLDIINQAADSTD
                     RIAALIAAASGGPPEDDKPTAPR"
BASE COUNT          256 a          459 c          569 g          253 t
ORIGIN      
        1 gcccgccgcc agcatcaggc ccagggccat tggatagccc tgcatccagc gcagctcggg
       61 catgtgttcg aagttcatgc catagacccc ggcgatcagg gtgggcggca ggaacacggc
      121 cgagaacacc gagaagatct tgaagatcgc gttctgctcg atgttgatca ggcccagggc
      181 cgcatccagc aggaaggtga tgttgctgga cacatagctg gaatggtcgg tgatcgactg
      241 cacgtcgcgt tgcagcgatt tgaggtgatc gcgcagctcg cggtcgcctt cgaactggtc
      301 ggccagagag gcgaagctga gcagacgggc caggctgacc aggctgtcgc gggccttgga
      361 attgatgttc tgggcgcggc ccaggcggtt gaggatctgc tcgaaggcgt cgccgcgcgg
      421 ccggctgaaa atggcgcggg actgggcctc gacctcgctg gcggtgcgct cgatgatgtc
      481 ggcggtgcgg tcgatgatgg cgtccagaag gcccaggaag gtctgcgggc cgccggggca
      541 caggctgggc tggcgctcgg cctgggcggc gaacaccgaa aaggcgcgcg gctcgacata
      601 gcggatggtg atcaggcgct ggccgatcag cacaaaggtc accggtgcgg ctgtgggcag
      661 gtcgccgtcg gcattgacca ggatggtggc ggtcatgaag gtgccgccgt cctcctgata
      721 caggcgcgag gaggcctcga tctcggccat ttcctcgcgg gtgggcagca gcaggccgat
      781 cgattgctcg accgaaacct cttcggtgcg cgaaggatcg accagctcga tccacaccgc
      841 gtcatcgggc aggcgccagt cggggccggc gcccgcatcc tcgaagcccg gagcgccaag
      901 gcgcagaatt ctcagcatgg atcagcgagc ctcgtcacgc gccgggaatg acccagcgcg
      961 agcaagacgt caactttggc gcggcttagc ggggcgcggt cggcttgtcg tcttcgggcg
     1021 gaccgcccga ggcggcggcg atcagggccg cgatccggtc ggtcgagtcg gcggcctggt
     1081 tgatgatgtc cagcggcgag gcgcgaaccg aggcggccac ctgattggcg gcggactttg
     1141 ccagcgaaac ggcggcgttc tgggccaggg ccagcttgct ggtcgcggcg gcgatcgtgg
     1201 tcgcctgggc gttctgatag atgaagccgt gggtcggata aacggtggtg cccagatcgc
     1261 ggatcggatc gtaccagacc ttgacctggc tccagtcgcc gttgggcgag acatccacca
     1321 ccgtcacgtc gcgttcgacc tggccgcggg ttccgttcat caccgaccag ttggcgtggg
     1381 tcacctggat gacgcggtcg gtcagcacct ggctgacgaa ggcgacgtgg cccaggttca
     1441 tgcggccggt cggcttgaag gaaaggacag agccgatctt cggcgcggag ccggtgtcgt
     1501 acttgccagc ggcctggccc caccaggtgc gagcgtc
//
LOCUS       DAOMEO010000877         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8334_length_1534_cov_3.156187, whole genome shotgun sequence.
ACCESSION   DAOMEO010000877 DAOMEO010000000
VERSION     DAOMEO010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_1534_cov_3.156187"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLH31_16425"
     CDS             complement(<1..356)
                     /locus_tag="PLH31_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQR90814.1"
                     /translation="MATATARIGEAPYATTITTGRHTLTADEPERLNGADVGPAPFDL
                     LLSALGACTSITLKMYAEHKAWPLEALEVRLHYHGDETPPRIERVLVPTGPLTDEQRK
                     RLADVAERTPVTLAIKG"
     gene            complement(390..>1534)
                     /locus_tag="PLH31_16430"
     CDS             complement(390..>1534)
                     /locus_tag="PLH31_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQR90815.1"
                     /translation="GGAASKALRAEGGLINLAHNIAPNGPARARLADTLTRLRRRSDL
                     LDNLDYAPSAGLEVQRRAGAAWLERSGGLAGADWTRLVCTAGAQQALALAFGAVVRWG
                     ETVLCEASTFYGVKALAEHMGYGLKGLAMDAQGLRPDALDEAASTGAFKAVAIQPTLQ
                     NPTGRIMSAGRRAQIVAVARKHDLMIIEDDIYAVYAPDAPPPFAVLAPERTFHVSGVS
                     KSLAPGLRTGFLIVPPGDAMDRVLRTVRALNYAPPNFGGLIATQWIEDGTADAIVAEI
                     QAEMTARLAMAREILGSSVETPMSASAPHVWLPMSELDAERLAGRALRGGVELTPPSA
                     PVVEPGLTTGVRLCLGAARDRDELERGLRIVAAAVADVDERSRAVI"
BASE COUNT          204 a          528 c          567 g          235 t
ORIGIN      
        1 ccctttatgg ccagggtcac cggcgtgcgc tcggcgacat cggccagtcg cttgcgttgt
       61 tcgtcggtta ggggacccgt cggaaccagc acgcgctcga tccggggcgg cgtttcatcg
      121 ccgtgatagt gcagtcgcac ttccagcgcc tccagaggcc aagccttgtg ctcggcatac
      181 atcttgagcg tgatcgaggt gcaggccccc agagccgaca gcagcagatc aaatggcgcg
      241 ggaccgacgt cggcgccgtt cagccgctcg ggctcgtcgg ccgtcagggt gtggcggccg
      301 gtggtgatcg ttgtcgcgta gggggcttcg ccgatgcggg cggttgcggt ggccatgggg
      361 cggctcctga ctggactctg tcggcgacct tagatcaccg ctcgcgaccg ctcgtccaca
      421 tcggccacgg ccgctgcgac gatcctcagg ccccgttcca gctcatcccg atcccgcgcc
      481 gcgcccaggc atagccgcac acccgttgtc aggcccggct ccaccaccgg cgcagagggc
      541 ggcgtgagct ccacgccgcc gcgtagggcc cggccggcca gccgctcggc gtccagttcc
      601 gacatcggca gccagacgtg cggggcgctg gccgacatcg gggtctcgac ggagctgccg
      661 aggatctccc gggccatggc caggcgggcg gtcatctcgg cctggatctc ggcgacgatg
      721 gcgtcggccg tgccgtcctc gatccactgg gtggcgatca gcccgccgaa attgggcggg
      781 gcgtagttta gggcgcggac ggtgcgcagc acgcgatcca tggcgtcgcc aggcgggacg
      841 atcaggaagc cggtgcgcag gcctggcgcc agggatttgg acacgcccga gacgtggaag
      901 gtccgctccg gcgccagcac cgcgaagggc ggcggggcgt ccggggcata gaccgcatag
      961 atgtcgtcct caatgatcat caggtcgtgc ttgcgggcca cggcgacgat ctgggcccgg
     1021 cggcctgcgc tcatgatccg gccggtgggg ttctgcaggg tgggctggat ggccacggcc
     1081 ttgaaggcgc cggtggaggc ggcttcgtcc agggcgtcgg ggcgcaggcc ctgggcgtcc
     1141 atggctaggc ccttcagccc atagcccatg tgctcggcca gcgccttgac gccatagaag
     1201 gtcgaggctt cgcacagtac cgtctcgccc cagcgcacca ctgcgccgaa ggccagggcc
     1261 agggcctgct gggcgccggc cgtacagacc agccgggtcc agtcggcgcc ggccaggccg
     1321 ccgctgcgct ccagccaggc ggcgcccgcg cggcgctgga cctccagtcc ggccgagggc
     1381 gcatagtcca gattgtcgag cagatcgctg cgccggcgca ggcgggtcag ggtgtccgcc
     1441 agtcgcgccc gggcggggcc gttgggcgcg atgttgtggg ccaggttgat caggccgccc
     1501 tcggctcgca gcgcttttga cgccgcgccg ccac
//
LOCUS       DAOMEO010000878         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8343_length_1533_cov_2.548714, whole genome shotgun sequence.
ACCESSION   DAOMEO010000878 DAOMEO010000000
VERSION     DAOMEO010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8343_length_1533_cov_2.548714"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>741)
                     /locus_tag="PLH31_16435"
     CDS             complement(<1..>741)
                     /locus_tag="PLH31_16435"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQR90816.1"
                     /translation="GSFGDAAENERARDMSDGVNIRRARIGMEGKVFSVFDYNMVYDF
                     GGSGVEEAGRVSSAWLQYSGYPVKVRVGFFPAPTSLEDASSFTGSLFVERASVAETVR
                     GLAGGDGRASIGLTASGERWNLAATVTGNTVTTLTYDEQLGFVGRAAYVPFKGKDWMI
                     HVGANANLIIDPAASGPDVALAGGAVTNVRLRDRPELRVDGARLIDTGNIDADGVTAL
                     GLELGGQIKNVYAQGEYFNIDVERRNSTL"
     assembly_gap    742..751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<752..997)
                     /locus_tag="PLH31_16440"
     CDS             complement(<752..997)
                     /locus_tag="PLH31_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQR90817.1"
                     /translation="MTHTTALRTALIAGVSFMTMAGAANAQTSAESEARIAALEAQLA
                     ALSGQIAELKAATAASPKDTRPAPGATTVTLSNGRPTI"
     gene            complement(1310..>1533)
                     /locus_tag="PLH31_16445"
     CDS             complement(1310..>1533)
                     /locus_tag="PLH31_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90818.1"
                     /translation="RQTLAGETGDTVAKFQNGSPFTLAALDDKQGALTLGFAIKAGTP
                     MSYLALEGGAEATKKQKRYNLRLSGRAMF"
BASE COUNT          248 a          490 c          528 g          257 t
ORIGIN      
        1 agcgtgctgt tgcggcgctc gacgtcgatg ttgaagtact cgccctgagc gtagacgttc
       61 ttgatctggc cgcccagctc caggcccagg gccgtgacgc cgtcggcgtc gatattgccg
      121 gtatcgatca ggcgcgcgcc gtcgacgcgc agttccggcc ggtcacgcag gcggacattg
      181 gtcacggcgc cgccggccaa ggcgacgtcc gggcccgagg cggccgggtc gatgatcagg
      241 ttggcgttgg cgcccacatg gatcatccag tccttgccct tgaacggcac ataggccgcg
      301 cgacccacaa agccgagctg ctcgtcatag gtcagggtgg tgacggtgtt gccggtgacg
      361 gtggccgcca ggttccagcg ctcgccgctg gccgtcaggc cgatgctggc ccggccgtcg
      421 ccgccggcca ggccgcgaac cgtttcggcg accgaggcgc gttcgacaaa cagcgaaccg
      481 gtgaagctgg acgcgtcttc caggctggtg ggggccggga agaagcccac gcgcaccttc
      541 accggatagc cgctgtactg cagccaggcc gacgagaccc taccggcctc ctccacgccc
      601 gagccgccga aatcgtagac catgttgtag tcgaagacgc tgaagacctt gccttccatg
      661 ccgatgcggg cgcggcggat attaacgccg tcgctcatat cccgggcgcg ctcgttctcg
      721 gcggcgtcgc cgaacgagcc cnnnnnnnnn ngatggtcgg gcgcccgttg gacagggtca
      781 ccgtggtggc gccgggcgcc gggcgcgtgt ccttggggct ggcggcggtc gcggccttca
      841 gctcggcgat ctggcccgac agggcggcca gctgggcttc cagcgcggcg atgcgggcct
      901 cggactcggc gctggtctgg gcgttggcgg cgcccgccat cgtcatgaac gagacgcctg
      961 cgatcagtgc ggtgcgcagg gcggtggtat gggtcatctt gatcgtcccc tctcggaccg
     1021 tctcggcggc ccggcctggc cgaactaggg aaaacgatta ttcctagttc gtgaatagag
     1081 attaggaaaa ccggccgtca gaaaatctgc aggcgcggtt tggcctcccg ggccgaagcc
     1141 gagaaacgcc agacaacaca aggggatata agtttaatga gcggttagtc atgcgtggac
     1201 aaatcgtcac agccgcgctt gccgcagggc ccaaaacgaa caagcctccc gccgcgatac
     1261 gcgacgggag gcttgaaatc agccggtttg gcgcgtgtag gccggtcgcc tagaacatcg
     1321 cccggccgga gaggcgcaga ttgtagcgct tctgcttctt ggtcgcctcg gccccgccct
     1381 caagggcgag atacgacatc ggcgtgccgg ccttgatcgc aaagcccagg gtcagcgccc
     1441 cctgcttgtc gtcgagggcg gcgagggtaa aggggctgcc gttctggaac ttggccaccg
     1501 tatcaccggt ttcacccgcc agggtctgac ggt
//
LOCUS       DAOMEO010000879         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8355_length_1532_cov_2.264049, whole genome shotgun sequence.
ACCESSION   DAOMEO010000879 DAOMEO010000000
VERSION     DAOMEO010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8355_length_1532_cov_2.264049"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLH31_16450"
     CDS             complement(<1..429)
                     /locus_tag="PLH31_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90819.1"
                     /translation="MQTATSMDDWRPGFAKRADNFHEVCDRAQAWRWEPGVSVPRILY
                     AVIVGTPELTIPQRMTLLVYAQHLNQDRLEQNQACVWPSSKLVARYLGCGVQTARAHR
                     RALESKGYMVRDYTRANRPAGEEAYDLAPLAARLEEMEDAD"
     assembly_gap    756..765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1143..1397)
                     /locus_tag="PLH31_16455"
     CDS             complement(1143..1397)
                     /locus_tag="PLH31_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HQR90820.1"
                     /translation="MARETKTTLALPKIDLEEKALDVRLRLKGKAAVDLADYQRAYAE
                     TNGHAVELDHLAAHILAAFIDGDRGFQAWRRSNPAPGARS"
BASE COUNT          261 a          468 c          530 g          263 t
ORIGIN      
        1 gtcagcgtcc tccatttcct ccaggcgagc cgcgagggga gcgaggtcgt aggcctcttc
       61 gccggccggc cggttggccc gggtatagtc gcggaccatg tagcccttgc tctccagggc
      121 ccggcgatgg gcgcgggcgg tctgcacgcc gcagccgagg tagcgggcca ccagctttga
      181 gctgggccac acgcaggcct ggttctgctc cagccggtcc tggttgaggt gctgggcata
      241 gaccagcagc gtcatccgct ggggaatcgt cagctccggc gtgccgacga tcacggcgta
      301 aaggatgcgc ggcacggaga cgccgggctc ccagcgccag gcctgggcgc ggtcacagac
      361 ctcatggaaa ttgtcggcgc gcttggcgaa gccaggccgc cagtcgtcca tcgatgtagc
      421 ggtttgcatg tcgtccctcg ggttgggacg aggccgtctg cacgggctga aacacgcagg
      481 agtcagaggc aaagctccgc catccggtcc ttgacgacgt tcaaaacgcg ccataggttg
      541 gaccgatctt cttgccgcca ttcacaacgg cactgcgatt tccgagagcc tccagcgcca
      601 actgggggct ttcgccgttt tagggtctcg tctaggtcac ggttcagatc ctggtcgggc
      661 ccggccaaga ccgggcgatc acgctgaggc agggcctcgg cgaggggtca cgggcctgaa
      721 ggcccggatc agacggtcag ggggagggca tggtgnnnnn nnnnnaagga caaggcccgc
      781 gccggcgtgt gctgttcgac ggccgcaagg aagcgtttgg ggtcttgacg ccaaaaacga
      841 aacgcctagc ctgtccccat caaacgccgc ccagaagcag cttgatcgaa tttcacgaga
      901 gccctcgatt gcagtcgggg gctttcgccg ttttgggcct catctcgcta agtcatcggg
      961 tctctccaag gcacgccacc ccgcgcgctg cacacgcgat tcggcagggt gaaggttggg
     1021 ttgagtctgc ccggcgccat aggccccggc aggggccaaa actgccgaaa tagcccctac
     1081 ggggagattc tctgttcttg cccagaatcg agaatcgagg cgtcagcgat ggcgaatcag
     1141 ctctaggagc gcgcgcctgg cgccggattg gatcgacgcc aggcctggaa gccccgatcg
     1201 ccatcgatga aggccgcgag gatgtgggct gccaggtgat cgagctcgac ggcatggccg
     1261 ttggtctcgg cataggcgcg ctggtagtcc gccagatcga cggcggcctt gcccttcagg
     1321 cgcagacgca cgtcgagcgc cttctcctcg aggtcgatct tcggcagggc gagggtcgtc
     1381 ttggtttcac gggccacgtc aggctccttc tgggggatgg aggaagcgga cgatcgccgt
     1441 actggcgaag tcggtgagct tcaggttgag gggatcgagg cggccgcgga tgcgagcgca
     1501 cagcaggccc gagacccgca ccgagatccg at
//
LOCUS       DAOMEO010000880         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8363_length_1531_cov_2.546070, whole genome shotgun sequence.
ACCESSION   DAOMEO010000880 DAOMEO010000000
VERSION     DAOMEO010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8363_length_1531_cov_2.546070"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>339
                     /locus_tag="PLH31_16460"
     CDS             <1..>339
                     /locus_tag="PLH31_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR90821.1"
                     /translation="HYRVAVPSAKGGLPEVNIGLLPGAGGTQRLPRIVGVEKALEMVT
                     SGQHVPAKAALAMGLFDELVEEGNLRAGAIAFAHKVLAEKRPLAKVRELNAKVEAARG
                     KPEIFAAFRAA"
     assembly_gap    340..349
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <350..>1531
                     /locus_tag="PLH31_16465"
     CDS             <350..>1531
                     /locus_tag="PLH31_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQR90822.1"
                     /translation="IEAAVNLPFDEGLKAERKLFMELMTGSQSAAQRHVFFAERQAAK
                     IPDVPDDTPIIPVKKVGVIGAGTMGGGISMNFLNAGIPVTIIEMKQENLDRGVATIRK
                     NYENTAKKGRITQDDVEARMALLTPSMNLEDLADCDLIIEAVFELMEIKKEVFGKLDK
                     IAKPGAILASNTSYLDIDVIAASTSRPESVIGLHFFSPANVMRLLEIVRGAKTDKSVI
                     ATSMQIGKKIGKVAVLVGNCHGFVGNRMLAQRQREAQKLILEGAMPWDVDRVLYDFGL
                     PMGPFAMSDLAGLDIGWDPAKTSSSTVREVLCEMDRRGQKNGKGFYDYDENRNAKPSP
                     IVEEVIRDFAEKRQIQRRDITDQEILERCLYPMVNEGAKILEEGKAIRASDIDTVWIN
                     G"
BASE COUNT          296 a          497 c          503 g          225 t
ORIGIN      
        1 cactatcgcg tcgccgtgcc ctcggccaag ggcggtctgc ccgaagtgaa catcggcctg
       61 ctgcccggcg ccggcggcac ccagcgcctg ccgcgcattg tcggcgtcga gaaggcgctg
      121 gagatggtca ccagcggcca gcacgtgccg gccaaggcgg ctctggcgat gggcctgttc
      181 gacgagctgg tcgaggaagg caatctgcgc gccggcgcga tcgccttcgc ccacaaggtg
      241 ctggccgaaa agcgcccgct ggccaaggtt cgcgagctca acgccaaggt cgaggccgcg
      301 cgcggcaagc ccgagatctt cgcggccttc cgcgccgccn nnnnnnnnng catcgaagcg
      361 gcggtgaacc tgcccttcga cgagggcctg aaggccgagc gcaagctgtt catggagctg
      421 atgaccggca gccagtcggc cgcccagcgc cacgtgttct tcgccgaacg ccaggccgcc
      481 aagatcccgg acgtgccgga cgacacccca atcatccccg tcaagaaggt gggcgtgatc
      541 ggcgccggca ccatgggcgg cggcatctcg atgaacttcc tcaacgccgg cattccggtg
      601 acgatcatcg agatgaagca ggaaaacctg gatcgcggcg tggccacgat ccgcaagaac
      661 tacgagaaca ccgccaagaa gggccgcatc actcaggatg acgttgaggc ccgcatggcg
      721 ctgctgacgc cgtcgatgaa cctcgaggat ctggccgact gcgacctgat catcgaggcc
      781 gtgttcgagc tgatggagat caagaaggag gtcttcggca agctggacaa gatcgccaag
      841 ccgggcgcga tcctggcgtc gaacacctcg tatctcgaca tcgacgtgat cgccgcctcg
      901 acctcgcggc cggaaagcgt gatcggcctg cacttcttct cgccggccaa tgtgatgcgc
      961 ctgctggaga tcgtgcgcgg ggccaagacc gacaagtcgg tgatcgccac ctcgatgcag
     1021 atcggcaaga agatcggcaa ggtcgccgtg ctggtcggca actgccacgg ctttgtcggc
     1081 aaccgcatgc tggcccagcg ccagcgcgag gcccagaagc tgatcctgga aggcgccatg
     1141 ccctgggatg tcgaccgggt gctctatgac ttcggcctgc ccatgggccc gttcgcgatg
     1201 agcgacctgg ccggcctcga catcggctgg gatccggcca agacgtcctc gtccaccgtg
     1261 cgcgaagtgc tgtgcgagat ggatcgccgg ggccagaaga acggcaaggg cttctacgac
     1321 tacgacgaga accgcaacgc caagccgtcg ccgatcgtgg aagaggtgat ccgcgacttc
     1381 gccgaaaaac gccagatcca gcgccgcgac atcaccgacc aggagatcct ggagcggtgc
     1441 ctgtatccga tggtcaacga gggggccaag atcctcgaag agggcaaggc catccgggcg
     1501 tccgacatcg atacggtgtg gatcaacggc t
//
LOCUS       DAOMEO010000881         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8384_length_1529_cov_2.069199, whole genome shotgun sequence.
ACCESSION   DAOMEO010000881 DAOMEO010000000
VERSION     DAOMEO010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8384_length_1529_cov_2.069199"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..625)
                     /locus_tag="PLH31_16470"
     CDS             complement(44..625)
                     /locus_tag="PLH31_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HQR90823.1"
                     /translation="MISQPAADAFVAELVDLFARLGDLRYGEDVSQLEHALQTAHHAR
                     VDDAPPALIVAALLHDIGHLMQKTGENAADLGIDTRHEHIGGGYLARAFGPDVTEPIR
                     LHVAAKRYRVAVDPAYFERLSAASLQSLALQGGPMSPDEITAFLAEPAAQDALRLRGY
                     DEAGKAPDADVAGLDAYHDLLRDQIYRAGRLSA"
     gene            851..>1529
                     /locus_tag="PLH31_16475"
     CDS             851..>1529
                     /locus_tag="PLH31_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR90824.1"
                     /translation="MQQPDHLHYLGVRNDIADRIDSGELKAGERLPSERQLQVGGGVA
                     RGTIREALFQLEAEGLVYRRNRSGWYVSPPPVVYDPTRWEGFMSYVEAQGRRPATETL
                     SKTVITCDAMLSQIFSRPIGAPLYWIRRRRSIDDRAVLIESIVVDASLAPGLIEHDLD
                     GSLTSVLKSVYGIGVARNKVDMRPCALTRGEAEALRVESGLPGLSVERTCYDAQGRVV
                     ELDREYWR"
BASE COUNT          262 a          492 c          511 g          264 t
ORIGIN      
        1 ggtctcggat gaggcggcgc cctagcaagc cgtaagttgc ggatcaggcc gacagccggc
       61 ccgcgcgata aatctgatcg cgaagcaggt catgataggc gtcaaggcca gccacgtccg
      121 cgtcgggggc cttgccagcc tcgtcataac cgcgcagtcg aagggcgtcc tgcgccgctg
      181 gctcggccag gaacgcagtg atctcgtccg gactcatcgg gccgccctgc agcgccagac
      241 tctgcaatga cgccgcgctg aggcgctcga aataggcggg gtccacagcc acccggtagc
      301 gcttggccgc cacgtgcagg cggatgggct cggtcacgtc tgggccgaac gcccgggcca
      361 gatagccgcc accaatatgc tcgtggcggg tgtcgatgcc caggtccgcg gcgttctccc
      421 cggtcttctg catcaggtgg ccgatatcat gcagcagggc ggcgacgatc agggcgggcg
      481 gcgcgtcatc gaccctggcg tgatgggccg tctgcagcgc gtgctccagc tggctgacat
      541 cctctccgta ccggaggtcg cccagtcggg cgaagagatc gacaagttcg gccacgaaag
      601 cgtcggccgc aggctgagag atcatagaca atccgggatc actgccctgt tcgcgccggc
      661 aagcgcgggc agggtttaaa attgggatta ctggtataga ccaaaataag ctatcgctgc
      721 gcgaatcttt cgtgacgcgc tcatcctgtc cacattgctc gccagggctc ggatcccgtt
      781 tcttgacctt ggcgtagcgc actcccatcc tcgcgcaaag cggcgctctg accgccgaca
      841 ggtccccacg atgcagcagc cagatcacct ccactatctt ggagttcgca acgacattgc
      901 cgatcggatc gacagtgggg aactgaaggc cggcgaacgt ctgccgtcgg aacgtcagct
      961 acaggtcggc ggcggggtgg cgcgtggcac gatccgcgag gcgcttttcc agcttgaggc
     1021 cgagggcctg gtctatcgcc gcaatcgcag cggctggtat gtctctcccc cgcctgtggt
     1081 ctatgatccg acgcgctggg aaggcttcat gtcgtatgtc gaagcgcagg gccggcggcc
     1141 ggcgaccgag acgctgagca agaccgtgat cacctgcgac gccatgctga gccagatctt
     1201 ttcccgcccc atcggcgcac cgctgtactg gatccgccgc cggcgatcga tcgacgaccg
     1261 ggccgtgttg atcgagagca tcgtggtcga cgcctccctg gcgccgggcc tgatagagca
     1321 cgatctggat ggctcgctga ccagcgtcct gaagtcggtc tatgggatcg gtgttgcgcg
     1381 caacaaggtc gacatgcggc cctgcgccct gacgcgcggc gaggccgaag cccttcgggt
     1441 ggagtcgggg ctgcccggat tgagcgtcga gcgcacgtgc tatgacgccc agggacgcgt
     1501 ggtcgagctc gaccgcgaat actggcgcc
//
LOCUS       DAOMEO010000882         1528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8391_length_1528_cov_2.739308, whole genome shotgun sequence.
ACCESSION   DAOMEO010000882 DAOMEO010000000
VERSION     DAOMEO010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8391_length_1528_cov_2.739308"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1185)
                     /locus_tag="PLH31_16480"
     CDS             complement(127..1185)
                     /locus_tag="PLH31_16480"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQR90825.1"
                     /translation="MSLHDLAARALHAFDPEDAHGLAIKALKAGLGPRDGAPDDPLLS
                     ITLAGMHLGSCVGLAAGFDKNAEVPDAMLAAGFGFVEAGTVTPLPQDGNPRPRLFRLT
                     QDQAVINRMGFNNEGLEVFAERLSRRAGRHGYVGANIGANKDATDRIQDYVTGLTRLW
                     GLSDYFTANISSPNTPGLRALQTRAALEELLGRLAETRAMLKAGSTSDYPIFLKVAPD
                     LEDGEVEAIVETVVAHGLDGIIVSNTTIARPQSLRSPQAGETGGLSGAPLLEPSTAVL
                     RRFHIAATGRCALIGAGGVADGAGAYAKIRAGARAVQLYSALVYGGPGLVSRIKRDLA
                     RRLRADGFATVEDAVGTA"
     gene            complement(1182..1526)
                     /locus_tag="PLH31_16485"
     CDS             complement(1182..1526)
                     /locus_tag="PLH31_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HQR90826.1"
                     /translation="MTLIYKILSRAEWEAAQATGRFDGSAVDVADGFIHLSAADQAQE
                     TAAKWFAGQADLVLLGVEAEALGPALKWEASRGGALFPHLYRPLLPAEVVSQASLDLD
                     ADGVPQLGGLTA"
BASE COUNT          240 a          540 c          537 g          211 t
ORIGIN      
        1 ccctgatccg cagctcctcc agcgagatgt gctcggctat cccgctggcc acggcccaga
       61 cgaacagggc gaccagggcc gccacgggcc cgaaacgtcg cagcagcgtc agggcgcggg
      121 tggtggtcat gcggtcccga ccgcgtcctc gaccgtggcg aaaccgtcgg cgcgcaaacg
      181 tcgggccaga tcgcgcttga tgcgcgacac caggccgggc ccgccataaa cgagggctga
      241 atagagctgc acggcccggg cgccggcccg gatcttggca taggccccgg cgccgtcggc
      301 gacgccgccc gccccgatca gggcgcatcg cccggtcgcc gcgatatgga agcgccgcag
      361 caccgccgtc gagggctcca gaagcggtgc gccggacagg ccgcccgtct cgccagcctg
      421 gggcgagcgc aggctttgcg ggcgggcgat cgtggtgttg ctgacgatga tcccgtccag
      481 cccatgggcg accacggtct cgacaatggc ctcgacctca ccatcctcaa ggtcgggcgc
      541 gaccttcagg aagatcggat agtcgctggt cgagccggcc ttcagcatcg cccgggtctc
      601 ggccagacgg cccagcagtt cctccagcgc cgccctggtc tgcagggcgc gcaggcccgg
      661 cgtgttgggc gaggagatgt tggcggtgaa atagtccgac agcccccaca ggcgggtcag
      721 gccggtgaca tagtcctgga tgcggtcggt cgcgtccttg ttggccccga tattggcccc
      781 gacatagcca tggcggccgg cgcgccgcga caggcgttcg gcgaagacct caagcccctc
      841 gttgttgaag cccatgcggt tgatcaccgc ctggtcctgc gtcaggcgaa acaggcgcgg
      901 gcgcggattg ccgtcctgcg gcaggggcgt gacagtgccc gcctcgacaa agccgaagcc
      961 ggcggccagc atggcgtcgg gcacctcggc gttcttgtcg aagccggcgg ccaggcccac
     1021 gcagctgccc agatgcatgc cggccagggt gatcgacagc agcggatcat ccggcgcccc
     1081 gtcgcggggc ccaaggccgg ccttcagcgc cttgatggcc aggccatggg cgtcttccgg
     1141 atcgaaggcg tgcagcgccc gggcggccag atcgtgcagg ctcatgccgt aagaccgccc
     1201 agttgcggca cgccgtcggc gtcgagatcg agggatgcct ggctgaccac ctcggccggc
     1261 agcaggggcc gatagagatg cggaaacagc gccccgcccc gcgaagcttc ccacttcagc
     1321 gccggaccca gggcctcggc ctcgacgccc agcagcacca ggtcggcctg gccggcgaac
     1381 catttggcgg cggtctcctg ggcctggtcg gcggccgaca ggtggatgaa gccatcggcc
     1441 acatccacgg ccgagccgtc aaaacgcccc gtcgcctggg cggcctccca ctcggcgcgg
     1501 gaaaggatct tgtagatcag ggtcattc
//
LOCUS       DAOMEO010000883         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8401_length_1527_cov_2.622962, whole genome shotgun sequence.
ACCESSION   DAOMEO010000883 DAOMEO010000000
VERSION     DAOMEO010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_1527_cov_2.622962"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLH31_16490"
     CDS             <1..772
                     /locus_tag="PLH31_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90827.1"
                     /translation="PTPPDFSGARLFRHDCAREAFVAAPDYPMPLDLLVGVERQKDRF
                     VENLKRFATGLPCNHVLLWGVRGTGKSSMTKAAFMAVSEDHADLKLVEVDRDEIAALP
                     PLFDLLRTRAERFVVLCDDLSFEDGAAAAKALKSALEGGVAGPPENVLFVATSNRRHL
                     MPRDHVESQGAIAAAENAEEEMSVSDRFGLWIGFPPMDQPTYLEAIRTYAERFDLVVE
                     TLDRRALQWSQLRGARSGRVAWQFIRDLAGELGKSLPA"
     gene            901..1149
                     /locus_tag="PLH31_16495"
     CDS             901..1149
                     /locus_tag="PLH31_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90828.1"
                     /translation="MGKPWFRTKQYGYGASLPCSWEGWAVLVLFIGVMIGLSALPVPL
                     TLPHRWLGVALRVGLIVGFIVLVWRKSDKPWGWRWGRK"
     gene            complement(1190..>1527)
                     /locus_tag="PLH31_16500"
     CDS             complement(1190..>1527)
                     /locus_tag="PLH31_16500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR90829.1"
                     /translation="TGQRNDGVNIRANAGAPVVSAADGEIAYAGNQVPTFGNLVLVKH
                     ADGWVTAYAHLGSTDVKMRQQVSKGQQLGTVGSTGGVNEPQLHFEMRYAPTVKDKARP
                     VDPALVLPR"
BASE COUNT          225 a          516 c          523 g          263 t
ORIGIN      
        1 gccgacgccg ccggatttca gcggcgcgcg gctgtttcgg catgattgcg cgcgcgaggc
       61 cttcgtcgcc gcgcccgact atcccatgcc gctggacctg ctggtcgggg tcgagcgaca
      121 gaaggaccgg ttcgtcgaga accttaagcg cttcgccacc ggcctgccct gcaaccacgt
      181 gctgctgtgg ggcgtacgcg gcacgggcaa gagctcgatg accaaggccg ccttcatggc
      241 cgtcagcgaa gatcacgccg acctgaagct ggtcgaggtg gatcgcgacg agatcgccgc
      301 cctgcccccg ctgttcgacc tgctgcgcac gcgcgccgag cgcttcgtgg tgctgtgcga
      361 cgacctgtcg ttcgaggacg gcgcagccgc cgccaaggcc ctgaagtcgg cgctggaggg
      421 cggcgtggcc ggcccgcccg agaacgtgct gttcgtcgcc acctccaacc gccgccacct
      481 gatgccccgc gaccacgtcg agagccaggg cgccatcgcc gccgccgaga acgccgagga
      541 ggagatgagc gtctccgacc gctttggcct gtggatcggc tttccgccca tggaccagcc
      601 gacctatctg gaggcgatcc gcacctatgc cgaacgcttc gacctggtgg tcgagaccct
      661 ggaccgccgc gccctgcagt ggtcgcaact gcgcggcgcg cggtccggcc gcgtggcctg
      721 gcagttcatc cgggatctgg cgggggagct ggggaagagc ctgccggcct agtttgcaag
      781 cccttcaacc ttgcccccct tcccccttga tgggggaagg gagaagaaag gagggtttca
      841 tgagacgcgg agcacggcct ggagtatagt tcgtgcgtag tttcaacccg gagggtggcc
      901 atgggcaagc cgtggtttcg gacgaaacag tatggctacg gcgcgagcct gccgtgcagt
      961 tgggaaggct gggcggtcct ggtcctgttc atcggcgtga tgatcgggct gtccgccctg
     1021 ccagtacccc tcaccctgcc tcaccgctgg ctgggcgtcg ccctgcgcgt gggcctgatc
     1081 gtcggcttta tcgtgctggt ctggcgcaag agcgacaagc catggggctg gcgctggggc
     1141 cgtaaatagc caaacggccc gcctccttcg aagcgggccg ttctctatcc tagcgcggca
     1201 ggaccagcgc cgggtcgacc ggccgggcct tgtccttcac cgtcggcgca tagcgcatct
     1261 cgaagtgcag ttggggctcg ttgaccccgc cggtcgaacc gacggtgccc agttgctgcc
     1321 ccttcgacac ctgctggcgc atcttcacgt cggtcgagcc cagatgggca taggccgtga
     1381 cccagccgtc agcgtgcttg accagaacaa ggttgccgaa ggtcggcacc tggttgccgg
     1441 cataggcgat ctcgccgtcg gcggccgaca ccaccggcgc gccggcattg gcgcggatgt
     1501 tgacgccgtc attgcgctgt cccgtgc
//
LOCUS       DAOMEO010000884         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8416_length_1524_cov_2.697073, whole genome shotgun sequence.
ACCESSION   DAOMEO010000884 DAOMEO010000000
VERSION     DAOMEO010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8416_length_1524_cov_2.697073"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLH31_16505"
     CDS             complement(<1..807)
                     /locus_tag="PLH31_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQR90830.1"
                     /translation="MADGQAPIDQDHFLGVSRSLSGRAWRERPGDSAAARSHQQVHGL
                     SEPLARALAARGVGVDGAEDYLRPTLKALFPDPSSFTDMDRAAEILIDALEAGRPTMV
                     FADYDVDGATSAAQLVRWFRHMGVELPIYIPDRLTEGYGPSPAAFKTIRDTGAELVVT
                     LDCGAAAYDAIASAATIGLEVVVIDHHLMREDPPAAAAVVNPNRPGCRSGQGVLAAAG
                     VTFVLLTALNREARKRGLFTDDRPQPDPRQWLDLVAMGEVCDVTQLVGSNR"
     gene            complement(907..>1524)
                     /locus_tag="PLH31_16510"
     CDS             complement(907..>1524)
                     /locus_tag="PLH31_16510"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQR90831.1"
                     /translation="NVSVGTIFSILPAPDGRETTEADFLLPGRQQVAAGYVVYGPQTM
                     LVLTLSKGVFGFTMSAEGDWLLTHESVSISADTAEFAINMSNQRHWAEPVRRYIDGCL
                     QGKDGPRGKNFNMRWVASMVADVHRILMRGGIFMYPWDAREPDKPGKLRLLYEANPMA
                     LIVERAGGKATDGKTAILDLQPAKLHQRVPVVLGSANEVDLVSAG"
BASE COUNT          251 a          514 c          521 g          238 t
ORIGIN      
        1 tcggttagag ccgaccagct gggtgacgtc gcagacctcg cccatggcca ccagatccag
       61 ccactggcgc ggatccggtt gcgggcggtc gtcggtgaac aggccgcgct tgcgggcctc
      121 cctgttgagg gcggtaagca gcacgaaggt gacgccggcg gcggccagaa ccccctgccc
      181 gctccggcag cccggacggt tgggattgac cacggcggcg gcagccggcg ggtcctcgcg
      241 catcaggtgg tgatcaatca ccacgacctc caggccgatc gtagcggcgc tggcgatggc
      301 gtcataggcc gccgcgccgc agtccagagt caccaccagt tcggcgccgg tgtcgcggat
      361 ggtcttgaag gccgcagggc tgggcccata gccctcggtc aggcggtcgg ggatatagat
      421 cggcagctcg acgcccatat ggcggaacca gcggaccagc tgggcggcgc tggtggcgcc
      481 gtcgacgtca tagtcggcga acaccatggt cggacggccg gcttccagcg cgtcgatcag
      541 gatctccgca gcccggtcca tgtcagtgaa gctggaagga tcggggaaca gcgccttcag
      601 ggtggggcgc agatagtcct cggccccgtc gacgcccacg cctcgagcgg ccagggcccg
      661 ggccagaggc tccgacaggc catgcacctg ctgatggcta cgcgccgccg ctgagtcgcc
      721 cggccgctcg cgccaggccc ggccgctcaa cgaacgcgag acgcccagga agtgatcctg
      781 atcgatgggc gcttgaccgt cggccatggc tagacggtcc tgagcttggg agcggtgagc
      841 atcgggcgag cctatccgtt tccggggatt cgctccatgc gccctagcga cgtcagtctc
      901 ggtcgcctaa cccgccgaga ccaaatcgac ctcgttggcc gagcccagca ccaccggcac
      961 ccgctgatgc agcttggccg gctgcaggtc gaggatcgcc gtcttgccat cggtcgcctt
     1021 gccacccgcg cgctcaacga tcagggccat cgggttagct tcatagagca ggcgaagctt
     1081 gccgggcttg tccggctcgc gggcgtccca cgggtacatg aagatgccgc cgcgcatcag
     1141 gatgcggtgg acatcggcca ccatcgaggc gacccagcgc atgttgaagt ttttgccgcg
     1201 cggaccgtcc ttgccttgca ggcacccatc gatgtagcgg cgcaccggct cggcccagtg
     1261 gcgctggttg gacatgttga tcgcgaactc ggccgtgtcg gcgctgatgg acacgctctc
     1321 gtgggtgagc agccagtcac cctcggcgct catcgtgaag ccaaacacgc ccttggacag
     1381 ggtcagcacc agcatggtct gcggaccgta gacgacataa ccagcggcga cctgctggcg
     1441 ccccggcaac aggaagtcgg cttcggtcgt ttcgcgccca tccggcgccg gcagaatcga
     1501 gaagatcgtc ccgaccgaga cgtt
//
LOCUS       DAOMEO010000885         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8421_length_1524_cov_1.858407, whole genome shotgun sequence.
ACCESSION   DAOMEO010000885 DAOMEO010000000
VERSION     DAOMEO010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8421_length_1524_cov_1.858407"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>895)
                     /locus_tag="PLH31_16515"
     CDS             complement(<1..>895)
                     /locus_tag="PLH31_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516388.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HQR90832.1"
                     /translation="GYFNDQKQTVAGLLAGRDQSLKDRVEWSKMRMDPTDILDVTGSV
                     YRYLINGQGSADNWTGLFTPGQRVRLRFVNAGAMTIFNVRLPGLTMSVVQADGQNVQP
                     VSVEEFQIAPAETYDVVVQPSEDRAFTLVAEASDRSGMARGTLAPRLGMSAPVPALRK
                     RPLGAMKDMGMDMSAHAGHDMSGMSMDMRDGANAPGVKLGPGVQTISPMPMDRLGEPP
                     QGLEGVGHKVLVYTDLVALDPNPDPRPPGRMVEIHLTGNMERFMWAFDGEAFGPLKKP
                     IAFQRDERARVVLVNDSMMAHP"
     assembly_gap    896..905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<906..1472)
                     /locus_tag="PLH31_16520"
     CDS             complement(<906..1472)
                     /locus_tag="PLH31_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HQR90833.1"
                     /translation="MQALDRRQMLAAATGLGISALLPTWARAGTGKAPQVLSGEDIRL
                     TIGHAPMTVDGKAGHAIAVNGTVPGPLIRLKEGQHVRLSVTNTLSEDTSIHWHGLLLP
                     FQMDGVPGVSFPGIKPGETFVYEFKVRQAGTYWYHSHSGLQEQEGVYGPIVIDPATAD
                     PVAYDREHVVVLSDYSFLHPHQLMRKLKA"
BASE COUNT          250 a          516 c          494 g          254 t
ORIGIN      
        1 cgggtgggcc atcatggagt cgttgaccag caccacccgc gcccgctcgt cgcgctggaa
       61 ggcgatgggc tttttcagcg gaccaaaggc ctcgccgtcg aacgcccaca tgaagcgctc
      121 catgttgccg gtgaggtgaa tctcgaccat gcggccgggc gggcgcggat cgggattggg
      181 gtccagcgcg acgaggtcgg tatagaccag caccttgtgg ccgacgcctt ccagaccctg
      241 cggcggctcg cccaggcggt ccatcggcat cggcgagatc gtctggacgc cgggccccag
      301 ctttacgccc ggcgcattgg cgccgtcgcg catgtccatg ctcatgcccg acatgtcgtg
      361 gccggcgtgg gcggacatat ccatgcccat gtctttcatg gcgccaagcg ggcgcttacg
      421 cagggccggg accggagcgc tcatgcccag gcgcggcgcc agggtccctc gcgccatgcc
      481 cgagcggtcg gaagcctcgg cgaccagcgt gaaagcgcga tcctccgagg gctgaacaac
      541 aacgtcatag gtctcggccg gcgcgatctg gaattcctcg acgctgaccg gctgcacgtt
      601 ctggccatcg gcctggacca cgctcatcgt caggcccgga aggcggacat tgaagatcgt
      661 catcgcgccg gcgttgacga acctcagccg cacccgctgg cccggcgtaa aaaggccggt
      721 ccagttgtca gccgacccct ggccattgat cagatagcga tagaccgagc ccgtgacgtc
      781 gaggatgtcg gtcgggtcca tccgcatctt cgaccattcg acgcggtcct tcagcgactg
      841 gtcgcggccg gccagcaggc ccgccaccgt ctgcttctga tcgttgaaat agccgnnnnn
      901 nnnnnggcct tcagctttcg catcagctga tgcgggtgca ggaagctgta gtccgacaac
      961 acaactacgt gctctcggtc ataggcgacc ggatcggccg tcgcgggatc gatgacgatg
     1021 gggccataga cgccctcctg ctcctgcagg cccgaatggc tgtggtacca gtaggtcccc
     1081 gcctgacgga ccttgaactc atagacaaag gtctcgcccg gcttgatccc cgggaagctg
     1141 acgcccggca cgccgtccat ctggaacggg agcagcaggc cgtgccagtg gatcgaggtg
     1201 tcctcggaaa gggtgttggt caccgacagc cggacatgct ggccttcctt caggcggatc
     1261 agcggaccgg gcacggttcc gttcaccgcg atggcgtggc ccgccttgcc gtcgaccgtc
     1321 atgggcgcat ggccgatggt caggcggata tcctcgccgc tcagcacctg cggcgccttg
     1381 cccgttcccg cgcgcgccca ggtcggcagc aatgccgaaa tgcccagccc cgtcgcggcg
     1441 gcgagcatct ggcggcgatc gagggcctgc ataacggtca tgtctccggt tcggccccga
     1501 acacaacgcg tccggctcca cacc
//
LOCUS       DAOMEO010000886         1523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8427_length_1523_cov_2.018392, whole genome shotgun sequence.
ACCESSION   DAOMEO010000886 DAOMEO010000000
VERSION     DAOMEO010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8427_length_1523_cov_2.018392"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1279
                     /locus_tag="PLH31_16525"
     CDS             35..1279
                     /locus_tag="PLH31_16525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQR90834.1"
                     /translation="MGPANTSRDDGQGNGNSMNFPPITALPQTPLLVVDKAALLRNIE
                     HMQALCDAAGVRLRAHGKTHKCSRLGRLIVERGAVGLCAQTVGEAEAFAAGGITDILV
                     TAAPPPWAAPRLAALARAGVTIGVTADDEGQIDHLSDAAVAAGTRLDLVVDLDLGTHR
                     TGAYPRDALHLARAADAAPGLRFAGLQAYLGHLQHMNDGAARKAADEAAFATLTALIV
                     ELTAAGLPPPVVTGGGTGTHAFDLASGVFTELQAGSYAVMDVEYDDCGPPGGGAWAFE
                     PSLYIAASVVSVRHKSHVVVDAGFKAVSMDGPPARVIAGAAPGSLWRPMGDEHGMIAH
                     PSMIDVLKAGGRDPLGFAGAVTAADADPARAFPADAPRVGDIVWLQPGHCDPTINLYD
                     ALHVWDGETLERWAVDGRRISS"
     gene            1359..>1523
                     /locus_tag="PLH31_16530"
     CDS             1359..>1523
                     /locus_tag="PLH31_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HQR90835.1"
                     /translation="MKRLLLATALIAAPMLAHAADAPKIDPANLAAHIKTLSSDAFEG
                     RGPATAGETKT"
BASE COUNT          223 a          580 c          507 g          213 t
ORIGIN      
        1 gtgttccatt ccgctccgca cgtgcggcgg acaaatgggt cccgcgaaca cgtcgcggga
       61 tgacggacaa gggaatggga acagcatgaa ctttccaccg atcaccgccc tcccccaaac
      121 cccgctcctt gtggtcgaca aggccgccct gctccgcaac atcgaacaca tgcaggccct
      181 gtgcgacgcc gccggcgtgc gactgcgggc ccacggcaag acccacaagt gctcgaggct
      241 gggccggctg atcgtcgaac gcggcgcggt cggcctttgc gcccagaccg tcggcgaggc
      301 cgaggccttc gcggcgggcg gcatcaccga catcctggtc accgcggccc cgccgccctg
      361 ggccgcgccg cgtctggcgg ctctggccag ggctggcgta acaatcggcg tgaccgccga
      421 cgatgaaggc cagatcgacc acctgtcgga cgccgccgtc gcggccggga ccaggctgga
      481 tctggtcgtc gatctggacc tggggaccca ccgcaccggc gcctatcccc gcgacgccct
      541 gcatctggcc cgcgccgccg acgccgcgcc gggcctgcgc ttcgccggtc tgcaggccta
      601 tctggggcac cttcagcaca tgaatgacgg cgcagcccgc aaggccgccg acgaagccgc
      661 cttcgcgacg ctgacggccc tgatcgtcga gctgaccgcc gctggcctgc ccccgcccgt
      721 ggtcactggc ggcggcaccg gcacccacgc cttcgacctg gcctcgggcg tgttcacgga
      781 gctgcaggcc ggctcctacg ctgtgatgga cgtggagtat gacgactgcg gcccgcccgg
      841 cggcggcgcc tgggcgttcg agccgtcgct ctacattgcc gccagcgtcg tctcggtccg
      901 ccacaagagc cacgtcgtgg tcgacgccgg cttcaaggcc gtgtcgatgg acggcccgcc
      961 ggcccgcgtg attgccggcg ccgcgccggg ctcgctgtgg cggcccatgg gcgacgagca
     1021 cggcatgatc gcccacccct cgatgatcga cgtgctgaag gccggcggtc gcgatccgct
     1081 gggctttgcg ggcgccgtga cggcggccga cgccgatccg gcccgcgcct tcccggccga
     1141 cgcgcccagg gtcggcgaca tcgtctggct gcagccgggc cactgcgatc cgaccatcaa
     1201 cctctatgac gccctgcacg tctgggacgg ggagacgctg gagcgttggg cggtggatgg
     1261 gcggcggatc agttcctgac cgcggcctct agccctcgct ggagaccccg gatacggtcc
     1321 cgccccaacg acaagatttg ctccaaagag tgacaacgat gaaacgcctg ctgctcgcca
     1381 ccgccctgat cgccgcgccg atgctggcgc acgccgctga tgcgcccaag atcgaccccg
     1441 ccaacctggc cgcgcacatc aagaccctgt cgtccgacgc cttcgaaggt cgcggccccg
     1501 ccacggccgg cgagaccaag acc
//
LOCUS       DAOMEO010000887         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8434_length_1522_cov_2.370143, whole genome shotgun sequence.
ACCESSION   DAOMEO010000887 DAOMEO010000000
VERSION     DAOMEO010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8434_length_1522_cov_2.370143"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLH31_16535"
     CDS             complement(<1..785)
                     /locus_tag="PLH31_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQR90836.1"
                     /translation="MANLTGRARRKNTYDAIVVGSGITGGIAAKELTQKGLKTLVLER
                     GPMVRHQQDYPTAMTAPWETKYPQGQLPQAELAAKYSVQRRTGYTMTEHTQHFFVRDD
                     ENPYVEENRFDWIRGYHVGGRSLTWGRQSYRHSPIDFEANAREGIGVDWPIRYEDLAP
                     WYDHVERFIGVSGQAEGLPHLPDGQYQPAMEMNCVEKAFKAKSEARFPERRITMGRTA
                     HLTEPTEEQMSLGRTKCQHRNLCIRGCPFGAYYSSNSGGLVAA"
     gene            1016..>1522
                     /locus_tag="PLH31_16540"
     CDS             1016..>1522
                     /locus_tag="PLH31_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQR90837.1"
                     /translation="MSALLGCLSLGVVSAASAEELKAGPWRSLFDGRTLDGWTPKIAK
                     HPVGKNHQQTFVVDQGAIRVSYAGYDTLNGQFGHLFYKTPFKAYRLRFTYRFLTEGAL
                     ADTPAWARSNSGVMFHSQSPASMMVEQSFPVSVEFQLLGKDGDAPRPTGAVCTPGITI
                     TFDGVKAKE"
BASE COUNT          246 a          494 c          505 g          277 t
ORIGIN      
        1 tcggccgcca ccagcccgcc ggagttggag ctgtaatagg cgccgaacgg acagccgcga
       61 atgcacaggt tgcggtgctg gcacttggtc cggccaaggc tcatctgctc ctccgtcggc
      121 tcggtcaggt gcgccgtgcg gcccatggtg atccggcgtt cggggaaccg cgcctcggac
      181 ttggccttga aggccttctc gacgcagttc atctccatcg ccggctggta ctggccgtca
      241 ggcagatgag gcagcccctc ggcctgaccc gacacgccaa tgaagcgctc gacgtggtcg
      301 taccacgggg ccaggtcctc atagcggatc ggccagtcga cgccgatccc ctcgcgggca
      361 ttggcctcga aatcgatcgg gctgtggcga tagctctggc gaccccaggt cagcgaccgt
      421 ccgcccacgt gatagccgcg aatccagtcg aagcggtttt cctcgacata ggggttctcg
      481 tcgtcgcgca cgaagaagtg ctgggtgtgc tccgtcatcg tgtagccggt gcggcgctgg
      541 acggagtatt tggcggccag ttcagcctgg ggcagttgcc cttgcgggta cttggtctcc
      601 cagggcgccg tcatggcggt gggatagtcc tgctggtggc gcaccatcgg cccgcgctcc
      661 agcaccagcg tcttgagccc cttctgggtc agctccttgg ccgcgatgcc gccggtgatg
      721 ccgctgccca cgacgatggc gtcataggtg ttttttcggc gcgcgcgacc ggtgagattg
      781 gccatgggtg ttccctcatc cgctcgctcg tggcgcgagg ccggcccgtt acagcccgac
      841 acgcaacctc ggcgaacccc ttgattgttg gcgaaaggct tccatgaaat gggttacatt
      901 gcaagaaagc tcggcaatcg acgtaagttg ttgggcgcgc gggaggaaac cagccatgac
      961 cacgtcagat cgttccacac aggttcgcca tgtaacagcc aggcgccgcg tcgcagtgag
     1021 cgccctgctc gggtgcctgt cgctgggcgt cgtcagcgcg gccagcgccg aggagctgaa
     1081 ggccgggccg tggcgctcgc tgttcgatgg ccgcacgctg gacgggtgga cgccgaagat
     1141 cgccaagcat cctgtgggta aaaatcatca gcagaccttc gtggtcgatc agggcgccat
     1201 ccgggtgtcc tatgccggct acgacacgct gaacggacag ttcggacacc tgttctacaa
     1261 gaccccgttc aaggcctatc gcctgcggtt cacctatcgc ttcctcacgg agggggcctt
     1321 ggccgacacg cccgcctggg cgcgcagcaa cagcggggtg atgttccaca gccagagccc
     1381 cgcgagcatg atggtggagc agtccttccc cgtgtcggtg gagttccagc tgctgggcaa
     1441 ggacggcgac gcgccccgcc ccaccggcgc agtctgcacg cccgggatca cgatcacctt
     1501 cgacggcgtg aaggccaagg ag
//
LOCUS       DAOMEO010000888         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8435_length_1522_cov_2.368098, whole genome shotgun sequence.
ACCESSION   DAOMEO010000888 DAOMEO010000000
VERSION     DAOMEO010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8435_length_1522_cov_2.368098"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLH31_16545"
     CDS             complement(<1..546)
                     /locus_tag="PLH31_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HQR90838.1"
                     /translation="MKDQRQGASSLPGRLRLLAGVASLVLAASAAHAQQPLVSAPAAP
                     VIRAPPSDGLGEEGYYLESDLLIRDDKSEILTARGDVEARYQGRTLRAEEVIYDTKAG
                     VITAKGKVALINADGTAQFADEMTLDKDLKAGFAMGFSARMDNNVKIAAVSAIRRNED
                     IQELNRAIYTPCEICAEKPTPT"
     gene            complement(569..>1289)
                     /locus_tag="PLH31_16550"
     CDS             complement(569..>1289)
                     /locus_tag="PLH31_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQR90839.1"
                     /translation="MTAQYETSRDAMMENYLKTAPKGTWLRQGDDKTQIVIRARARDQ
                     VDGSVRLRGVSLFVYTLNNKGVMDFSRRIEANEARLEPGFWRLIGVREATPGAGAIRS
                     ESLSIPSNLDDRTASERFNSPQAVALWRLPTTIKRTEDAGFSATPFRLRYHQILATPL
                     LFAAMSVLAAAFSLRLMRLGGLAALAGSGVALGFGFFFFNELCSALAKAEVLHPAIAA
                     WTPPLVALLAGFTLLCYTEDG"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1300..>1522)
                     /locus_tag="PLH31_16555"
     CDS             complement(<1300..>1522)
                     /locus_tag="PLH31_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQR90840.1"
                     /translation="FQLLYLTILQAPATILVLTPFIFLFGTLGAFVGLNRRSELIAMR
                     AAGVSAWRFILPAAVAAFVIGVLTITLLNP"
BASE COUNT          270 a          481 c          526 g          235 t
ORIGIN      
        1 ggtgggggtc ggcttttcgg cgcagatttc gcagggcgtg tagatcgccc ggttgagctc
       61 ctggatgtct tcgttgcgcc ggatggccga gacggcggcg atcttgacgt tgttgtccat
      121 gcgggccgag aagcccatgg cgaagccagc cttcaggtcc ttgtccagcg tcatctcgtc
      181 ggcgaactgc gccgtcccgt cggcattgat caaagcgacc ttgcccttgg cggtgatgac
      241 gccggccttg gtgtcgtaga tcacttcctc ggcgcgcagg gtgcggccct ggtagcgcgc
      301 ctcgacatcg ccgcgcgcgg tcaggatctc gcttttgtcg tcgcggatca gcaggtcgga
      361 ttcgagatag tagccctctt cgcccaggcc gtcggacggc ggcgccctga tgaccggcgc
      421 ggcgggcgcg ctgaccagcg gctgctgggc atgcgctgcg gaagcggcca gcaccagcga
      481 ggcgacaccg gcgagcagac gcaagcgacc cggcagggac gacgcgcctt gacgctgatc
      541 cttcatgaac gacctcggag acacggactc aaccatcctc ggtataacaa agcagagtga
      601 aacccgccaa aagcgcgacg agcgggggtg tccaggcggc gatcgccggg tggagaacct
      661 cggctttcgc aagcgcgctg catagctcgt tgaaaaagaa aaatccgaag cccagcgcaa
      721 cgccggatcc ggccagggcc gcaaggcccc ccagacgcat caggcgcagc gaaaacgctg
      781 ccgccagcac cgacatggcg gcgaacagca gcggggtcgc caggatctgg tgatagcgca
      841 gccggaatgg cgtggccgag aagccggcgt cctcggtccg cttgattgtg gtgggcaggc
      901 gccacagcgc gacggcctga ggcgagttga agcgctccga ggcggtgcgg tcgtcgagat
      961 tggacgggat cgacaggctt tcggagcgaa tcgccccagc gcctggagta gcctcccgca
     1021 cgccgatcag ccgccagaag ccgggctcca gccgggcctc gttggcctcg atccggcgcg
     1081 agaagtccat cacgcccttg ttgttcaggg tgtagacgaa cagcgacacg ccgcgcaggc
     1141 gcaccgaacc gtccacctgg tcgcgcgccc gggcccggat cacgatctgg gtcttgtcat
     1201 cgccctggcg cagccatgtg cccttgggcg cggtcttcag atagttttcc atcatcgcgt
     1261 cgcgcgaggt ttcgtattgc gcggtcatgn nnnnnnnnnc ggattgagca gggtgatggt
     1321 caggaccccg atcacgaagg ccgccaccgc cgccggcagg atgaaccgcc aggccgaaac
     1381 gccggccgcg cgcatggcga tcagctcgct gcgccggttc aggccgacga aggcccccag
     1441 ggtgccgaac agaaaaatga agggcgtcag caccaggatg gtggccggcg cctgcaggat
     1501 ggtcaggtac agcagctgga ag
//
LOCUS       DAOMEO010000889         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8436_length_1522_cov_2.034765, whole genome shotgun sequence.
ACCESSION   DAOMEO010000889 DAOMEO010000000
VERSION     DAOMEO010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8436_length_1522_cov_2.034765"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLH31_16560"
     CDS             <1..322
                     /locus_tag="PLH31_16560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQR90841.1"
                     /translation="ARETATGRYVGEVGCADFHREIEPSFFGEPERGWVLAAWSHGQG
                     FGTEAVTAGLAWIDQALAPPRVPCIIDPGNAASLALARKTGFKIVTHTSYKDAPIMLL
                     ERTR"
     gene            complement(319..1359)
                     /locus_tag="PLH31_16565"
     CDS             complement(319..1359)
                     /locus_tag="PLH31_16565"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQR90842.1"
                     /translation="MTSAATSSFAYVPHPKPTSAEQRAALLADPGFGRVFTDHMVTIR
                     YSQAQGWHDAKVEPRGPIPMDPASSVLHYAQEIFEGMKAYRTADGGTALFRPEENARR
                     FQDSARRMAMAELPETLFVEAVEKLIDIDRDWIPDGEGSLYLRPFMFATEVFLGVKPA
                     SEYLFVVLASPVGAYFKNKASAISVWVSETYTRAALGGTGAAKCGGNYAASLLAQSEA
                     IAHGCDQVVFLDAVERRWVEELGGMNVFFRMDDGSLQTPPLGGTILPGITRNSIITLA
                     RDQGITVREEPYSLDQWRADAAGIGTVRTASGDFTIGNGAEGETTEQLRNALIGIQRG
                     RTADPYGWVRPV"
BASE COUNT          251 a          496 c          531 g          244 t
ORIGIN      
        1 ggcgcgcgag acggcgacag ggcgctatgt cggcgaagtc ggctgcgccg acttccaccg
       61 tgagatcgag cccagcttct ttggcgagcc ggaaaggggc tgggtgctgg cggcctggtc
      121 gcacggccag ggctttggga ccgaggcggt gacggcaggc ctggcctgga tcgatcaagc
      181 cctggcgccg ccccgcgtgc cctgcatcat cgatccaggc aacgccgcat ccctggccct
      241 ggcccgcaag accggcttca agatcgtgac ccacacatcc tataaggacg cgccgatcat
      301 gctgctggaa cggacccgct aaaccgggcg aacccagcca taggggtcgg cggtgcggcc
      361 gcgctggatg ccgatcaggg cgttgcgcag ttgttcggtc gtctcgccct cggccccgtt
      421 gccaatggtg aagtcgcccg aggcggtgcg caccgtgccg ataccggccg cgtcggcccg
      481 ccactggtcc agcgaatagg gctcctcgcg caccgtgatc ccctggtcgc gcgcgagggt
      541 gatgatcgag ttgcgggtga tgccgggcag gatcgtgccg cccagcggcg gggtttgcag
      601 gctgccgtcg tccatccgga agaagacgtt catgccgccc aactcctcga cccagcggcg
      661 ctcgaccgcg tcgaggaaca ccacctggtc gcagccgtgg gcgatggctt cggactgggc
      721 cagcaggctg gcggcatagt tgccaccgca cttggccgcg cccgtgccgc ccagcgccgc
      781 gcgcgtgtag gtctctgaaa cccagaccga gatcgccgac gccttgttct tgaaataggc
      841 cccaactggc gaggcaagga cgacgaacag atattcgctg gccggcttga cgcccagaaa
      901 tacctcggtg gcgaacatga agggccgcag atacaggctg ccctcgccgt ccgggatcca
      961 gtcccggtcg atgtcgatca gcttctcgac cgcttcgacg aacagggtct cgggcagctc
     1021 ggccatggcc atgcgacgcg ccgagtcctg gaagcggcgg gcgttctctt cgggccggaa
     1081 cagggccgtg ccgccgtccg ccgtgcgata ggccttcatg ccctcgaaga tttcctgggc
     1141 gtagtgcagc accgacgaag ccggatccat cggaatgggc ccgcgcggct cgaccttggc
     1201 gtcatgccag ccctgcgcct gggaatagcg gatggtcacc atgtggtcgg tgaacacccg
     1261 gccaaaaccc ggatcagcca gcagggcggc gcgctgctcg gcggaggtcg gcttgggatg
     1321 cggaacgtag gcgaacgatg aggtggcggc tgaggtcatg tggggcttgg ctctcgctcg
     1381 gaaaggtggg tcgcggcgcg agcacgccgc aagggtggaa tggatatggc tgatgcttcg
     1441 cgtcaagtgc cacgcacgca aagaccgcat ccgccctcag gttcgcaagc cctgtgtggc
     1501 tagatatcgc cgccgacgtt gg
//
LOCUS       DAOMEO010000890         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8484_length_1516_cov_1.702943, whole genome shotgun sequence.
ACCESSION   DAOMEO010000890 DAOMEO010000000
VERSION     DAOMEO010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_1516_cov_1.702943"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLH31_16570"
     CDS             complement(<1..776)
                     /locus_tag="PLH31_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQR90843.1"
                     /translation="MTLLVGEGEILIGLGEGPVTQRLDRSNRHGVVAGATGTGKTVTL
                     QIMAQAFSDAGVPVFAADVKGDLSGIAIAGTPNEKMLARAASMDLTLTPAAPPTVFWD
                     LFGQKGHPIRTTISEMGPLLLARLLELNDVQEGVLTIVFHVADKDGLLLLDLKDLQAC
                     LKYVADHASEIGTLYGNVSPATVGSIQRKLLTLQSQGADNFFGEPALRLADIMRTDVA
                     GRGYVNLLAADKLIHSPKLYSTFLLWLLSELFEELPELGDP"
     gene            874..>1516
                     /locus_tag="PLH31_16575"
     CDS             874..>1516
                     /locus_tag="PLH31_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQR90844.1"
                     /translation="MTVSSSAATRNAVVFLAVIAGGAALLWMRDILTPLALAVFLAVM
                     IDGFARVLMAKARFAKRLALPTAIVLSISLFGLSVWVVAENSASFVDQLKDYAPRLND
                     VIARLAHALGIQVAPTVADLITQLNPQRYIGAAAQSLQNFASSAILVLIYLGFIIASR
                     RGFGRKIVAMFPHHGERDKAMVLFHRIRDGVEQYLWIQTVTGLIIAIGAWLVMA"
BASE COUNT          258 a          482 c          490 g          286 t
ORIGIN      
        1 ggatcgccca gctcgggcag ttcctcgaac agctccgaca gcagccacag caggaaggtc
       61 gaatagagct tgggcgaatg gatcagcttg tcggcggcca gcaggttcac atagccgcga
      121 ccggcgacgt cggtgcgcat gatgtcggcc agcctcaggg ccggctcgcc aaagaagttg
      181 tcggcgccct gactctggag ggtcagcagc ttgcgctgga tcgaccccac cgtggcgggc
      241 gagacattgc cgtagagcgt gccgatctcg gaggcgtggt cggcgacata tttcaggcag
      301 gcctgcaggt ccttgaggtc cagaagcagc aagccatcct tgtcggcgac atggaagacg
      361 atggtcagaa cgccttcctg aacgtcgttg agctccagaa gccgcgcgag cagcagcggg
      421 cccatctccg agatggtggt gcggatcgga tgacccttct ggccgaaaag gtcccagaac
      481 acggtcggcg gcgcggccgg ggtcagggtc aggtccatcg aggcggcgcg ggccagcatc
      541 ttctcgttgg gcgttccggc gatggcgatg ccggaaagat cgcccttcac gtcggcggcg
      601 aacacgggca cgccagcgtc ggagaaagct tgagccatga tctgcagggt aacggtcttg
      661 ccggtgccgg tcgcgccggc cacgacgccg tgtcggttgg agcgatccag cctttgcgtc
      721 acgggacctt cgcccagtcc gatcaggatc tcgccttcac caaccaacag cgtcatgtgc
      781 aacatccttc aaactgaagc actgacccta accgcgccct tgcgccgcgc caatcgcggt
      841 aggcaaagtg ttgtgatctg caggagttct tgaatgaccg tttcgtcgtc cgccgccacg
      901 cgcaacgccg ttgtgttcct ggcggtcatc gcgggcggcg cggcgctgct gtggatgcgc
      961 gacatcctca cgccgctcgc cctggccgtg ttcctggcgg tgatgatcga cggcttcgcc
     1021 cgcgtgctga tggccaaggc ccgctttgcc aaacgcctcg ccctgcccac ggccatcgtg
     1081 ctgtcgatct cgctgtttgg cctgtcggtc tgggtcgtgg cggagaacag cgccagcttc
     1141 gtcgaccagc tcaaggacta tgcgccaagg ctgaatgacg tcatcgccag gctggcccac
     1201 gcgctgggca ttcaggtggc gccgacggtc gccgacctga tcacccagct caacccgcag
     1261 cgctatatcg gcgccgccgc ccagagtttg cagaacttcg cctcaagcgc gattctggtt
     1321 ttgatctatc tgggattcat catcgcctcg cgtcgcgggt ttggccgcaa gatcgtggcc
     1381 atgtttccgc atcatggcga gcgcgacaag gcgatggtcc tgttccatcg catccgtgac
     1441 ggcgtcgaac agtatctgtg gatccagacg gtcaccggcc tgatcatcgc catcggggcc
     1501 tggctggtca tggctt
//
LOCUS       DAOMEO010000891         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8488_length_1514_cov_2.389993, whole genome shotgun sequence.
ACCESSION   DAOMEO010000891 DAOMEO010000000
VERSION     DAOMEO010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8488_length_1514_cov_2.389993"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLH31_16580"
     CDS             complement(<1..417)
                     /locus_tag="PLH31_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HQR90845.1"
                     /translation="MHASNRTLRHTLSLSCGVAALGFALAAAPAAMAQDTQVGEVIVT
                     AQKRSENLQDVPVSVTALGGERLQSTFAAGDDILALSAKAPGLYAESSNGRAAPRFYI
                     RGLGNSDFDLAASQPVSIIQDDVVLENVVLKSSPIYD"
     gene            complement(719..>1514)
                     /gene="glnA"
                     /locus_tag="PLH31_16585"
     CDS             complement(719..>1514)
                     /gene="glnA"
                     /locus_tag="PLH31_16585"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517420.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQR90846.1"
                     /translation="MKPEKHHHEVAPAQHELGLKFDTMVVMADRLQLYKYVIHNVAHA
                     YGKTATFMAKPMFGDNGSGMHVHQSIWQDGKPLFAGDKYAGLSDMCLWYIGGIIKHAK
                     AINAFSNSTTNSYKRLVPGYEAPVKLAYSARNRSASIRIPHVDSPKAKRLEARFPDPM
                     GNPYLTFVALLMAGLDGINNKIDPGAPADKNLYDLPPREQKKIPEVCGSLREALENLD
                     KDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFDMYYKC"
BASE COUNT          256 a          419 c          519 g          320 t
ORIGIN      
        1 gtcgtagatg ggggagctct ttaggacgac gttctccaga acgacgtcgt cttggatgat
       61 cgacacgggt tgcgaggcgg caaggtcgaa gtcgctgttg cccaggccgc ggatgtagaa
      121 gcggggcgcc gcgcgaccat tggaggattc ggcgtagagg ccgggggcct tggccgagag
      181 ggccaggatg tcatcgccgg cggcgaaggt cgattgcagg cgctcgccac ccagggcggt
      241 caccgagaca gggacgtcct gcaggttttc gctgcgcttt tgggcggtga cgatgacttc
      301 gccaacctgg gtgtcctgag ccatagccgc tggcgctgcg gcgagtgcaa agcccagggc
      361 tgcgacaccg caggacaggc tgagggtgtg acgaagggtg cgattggagg cgtgcatgga
      421 gagatcctga tgctgatccg agtctcgcgt cgccgagtct tcggggcgtt cgcgaagctt
      481 gcgttgcggt gcggtaagcc cagttggcgc tcctgcgata tggttcgcca tgtcatttcg
      541 cccattgccg gccgattgcg acccttctgt tgcgaagttg cgacaatttt gttcagctgg
      601 cgcccataag taagaatcca ttctcaaagt cgccgcacgg acgccttcgg ccgactaaaa
      661 tgaaaagggc cggagattgc tctccggccc tggtcactag tcctaggtgt cgttcaagct
      721 agcacttgta gtacatgtcg aattcgacgg ggtgcgggtg cagctgcagg cgcatcactt
      781 cttccatctt cagctcgatg tagctgtcga tgaagtcgtc atccatgacg ccgccggcct
      841 tcaggaaggc gcggtccttg tcgaggttct cgagcgcctc gcggagcgaa ccgcagactt
      901 ccgggatctt cttctgctcg cgcgggggca gatcgtagag gttcttgtcg gcgggcgcgc
      961 ccggatcgat cttgttgttg ataccgtcca ggccagccat cagcagcgca acgaaggtca
     1021 gataggggtt gcccatcggg tcggggaagc gggcttccag acgcttggcc ttcggcgagt
     1081 cgacgtgcgg gatacggatc gaggccgagc ggttgcgggc cgaataggcc agcttcaccg
     1141 gggcttcgta gccgggcacc aggcgcttgt agctgttcgt cgtggagttc gagaatgcgt
     1201 tgatcgcctt ggcgtgcttg atgatgccgc cgatgtacca caggcacatg tccgacaggc
     1261 cagcgtactt gtcgccggcg aacagcggct tgccgtcctg ccagatcgac tggtggacgt
     1321 gcatgcccga accattgtcg ccgaacatcg gcttggccat gaaggtggcc gtcttgccat
     1381 aggcgtgggc cacgttgtgg atcacgtact tgtagagctg cagacggtcg gccattacga
     1441 ccatggtgtc gaacttcagg ccaagctcat gctgggcggg cgccacttcg tggtggtgct
     1501 tttccggctt catg
//
LOCUS       DAOMEO010000892         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8496_length_1513_cov_1.995199, whole genome shotgun sequence.
ACCESSION   DAOMEO010000892 DAOMEO010000000
VERSION     DAOMEO010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_1513_cov_1.995199"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLH31_16590"
     CDS             complement(<1..643)
                     /locus_tag="PLH31_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HQR90847.1"
                     /translation="MSAPRPISEDDLQAYVDEALSTTRREEVEAYLDAHPEEAKRIGG
                     FVADRAALRAALGAIAEAPIPSELGLRALIEARRTRQAYWRPAIAAAAVLMIGGLAGW
                     TARGVVTPPINGVGALSREAADNYRVYASDVHRPVEIGSAQKAELVRWVSNRVSSSVE
                     VPDLQAAGYRFIGGRLVSTPHGPAALFIYDDAQGLRLAVMVRPMRVEKNTAMSE"
     gene            complement(640..>1045)
                     /locus_tag="PLH31_16595"
     CDS             complement(640..>1045)
                     /locus_tag="PLH31_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR90848.1"
                     /translation="GRWSQRKEASDARSWLFSILHNLAISRWRRLGRRGREVSLDELN
                     EADLAERATQQDSVNQSDILRSLAALPQEYRSVLLLVGVEDLSYAQAAKVLDVPIGTV
                     MSRLSRGRARLAQIMANGSATQVQTPHLRRIK"
     assembly_gap    1046..1055
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1172..>1513)
                     /locus_tag="PLH31_16600"
     CDS             complement(1172..>1513)
                     /locus_tag="PLH31_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90849.1"
                     /translation="SAHLVLGPIGGGGEEPTSVSLFDQGLLQVLQASVTGLTPKQAYV
                     LALARQADGSGPLEPLASFMTNPAGAAIVNTIGPIRQVVQAASTTDRRYLVIAPGTAD
                     QFGTPVQVQAR"
BASE COUNT          232 a          511 c          523 g          237 t
ORIGIN      
        1 gttcggacat ggcggtgttt ttctccaccc gcatggggcg gaccatcact gccagcctga
       61 ggccttgggc gtcgtcatag atgaacaggg ccgccgggcc gtgcggcgtg gagacgaggc
      121 ggccgccgat gaagcgataa cccgccgcct ggagatcggg cacctcgacc gagctgctga
      181 cacggttgga gacccaacgc accagctcgg ccttttgggc ggagccgatc tcgacggggc
      241 gatgaacgtc cgaagcatag acccggtagt tgtcggccgc ctcgcgcgac agggcgccaa
      301 ccccgttgat cggcggggta acaacgcccc tggccgtcca tccggccagt ccgccgatca
      361 tcagtacggc cgccgcggcg atcgcgggcc gccagtaggc ctgacgcgtg cgacgagcct
      421 cgatcaaggc gcgcaagcct agctccgacg ggatcggcgc ctcggcgatc gcgcccaggg
      481 cggcgcgcaa ggcggcgcga tcggcgacga agcccccgat cctcttggct tcttcggggt
      541 gtgcgtcgag ataggcctcc acctcctcgc gacgcgtcgt ggagagagcc tcgtcgacat
      601 aagcctgcag gtcgtcttcg ctgatggggc gcggggcgct catttgatcc tccgcagatg
      661 gggcgtttga acttgggtgg ccgaaccatt ggccatgatc tgggcaaggc gcgcgcgacc
      721 gcgcgacagc cgcgacatca ccgtgccgat cggcacgtcc agcaccttgg cggcctgggc
      781 gtaggagagg tcctcgaccc cgaccaacag gagcaccgag cggtactcct gcggcaaggc
      841 cgcgagggac ctgaggatgt cgctctggtt cacgctgtcc tgctgggtgg cgcgctcggc
      901 cagatccgcc tcgttgagct cgtccaacga cacttctcgc ccgcgacggc ccagacggcg
      961 ccaacggctg atcgccagat tgtgcaggat cgagaagagc caactgcgcg cgtcgctggc
     1021 ctccttgcgc tggctccagc ggcctnnnnn nnnnnaggtc gtcagcctcg gcggcgtccc
     1081 ggaccagcgc gcgcgcatag cgtcgcaggc tcgggattga gggttcgatc aggcggatca
     1141 tgtcgtccac ggcgggtctc cgggtcgcgc cctagcgggc ctgaacctga acgggcgtgc
     1201 cgaactggtc ggcggttcct ggcgcgatca ccagatagcg cctgtctgtc gtgctcgctg
     1261 cctgcacgac ctggcggatg ggcccgatcg tattgacgat cgccgcgccg gccggattgg
     1321 tcatgaagct ggccagcggc tcgagcgggc cagagccgtc agcctgccgc gccagggcca
     1381 ggacataggc ttgcttgggc gtcaggcccg tgaccgacgc ctgcagcacc tgcagcagcc
     1441 cctggtcgaa caggctcacg ctggtgggtt cctcgccgcc gccgccgatg ggacccaaca
     1501 ccagatgcgc gct
//
LOCUS       DAOMEO010000893         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8502_length_1512_cov_2.514756, whole genome shotgun sequence.
ACCESSION   DAOMEO010000893 DAOMEO010000000
VERSION     DAOMEO010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_1512_cov_2.514756"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1120
                     /locus_tag="PLH31_16605"
     CDS             98..1120
                     /locus_tag="PLH31_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR90850.1"
                     /translation="MSSKITIHDVARQSGVSIKTVSRVLNREPNVKADTRDRVQAAVA
                     ALRYRPNISARSLAGSKAYLIGVFFDNPSPGYITDVQLGAIARCREEGYHLIVEPLDS
                     TADDIEDQVAPMLATLRMDGVILTAPVCDHPVVLAALEREAVPYVRISPGSELDRSAR
                     VGMDDRLAAYQMTRHLIELGHRDIGFIIGHPDHGASHLRHEGFLAAMADSGLTVRPER
                     VEQGYFSFRSGFEAAERLLTGDDRPTAVFASNDDMALGVMAVANRLRLNVPETLSVAG
                     FDDTPGAKVVWPQLTTVRQPIAAMAAAAADILLSGVSWEEGALPPSKLLDFELVVRES
                     TGPAVT"
     gene            1147..>1512
                     /locus_tag="PLH31_16610"
     CDS             1147..>1512
                     /locus_tag="PLH31_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90851.1"
                     /translation="MLTALSRCAVIPPSKNVALKRRLEETPSRCPFRLTAFIDGHRLQ
                     PGASRRVLLDAAHLRAASSLFCSPDRRRRTTGSFMAAYRPFAQASVLALLMAGSLGAA
                     AFAQTPSAVANPELWPKAAS"
BASE COUNT          224 a          516 c          499 g          273 t
ORIGIN      
        1 agcgagcgtg gcggccgaaa gtgggagccg ttttcggccc cggccacgct ccagataaga
       61 tttggagtcc tgatgggctc tggggcgagg ggcggaattg agctcgaaga tcacgatcca
      121 cgatgtcgcc cggcaatcgg gcgtctcgat caagaccgtc tcgcgggtcc tcaatcgcga
      181 gcccaacgtc aaggccgaca cccgcgatcg ggtgcaggcg gcggtggcgg cgctgcgcta
      241 tcgacccaat atctcggccc gcagcctggc gggttcgaag gcctatctga tcggcgtctt
      301 cttcgacaac cccagccccg gctacatcac cgatgtgcag ctgggcgcca tcgcgcggtg
      361 ccgggaggag ggctatcacc tcatcgtcga gccgctcgac tcgaccgccg acgatatcga
      421 ggatcaggtc gcgccgatgc tggcgaccct gcggatggac ggcgtcatcc tgaccgcacc
      481 ggtctgcgac caccctgtgg tgctggcggc gctggaacgg gaagccgtgc cctatgtgcg
      541 tatttccccc ggcagtgagc tggatcgttc cgcacgcgtg ggcatggatg atcgcctggc
      601 cgcctatcag atgaccaggc acctgatcga gctgggccac cgcgatatcg gcttcatcat
      661 cggccaccct gatcacggcg cctcccacct ccggcatgag ggcttcctgg ccgccatggc
      721 cgacagcggc ctgacggtcc gccccgagcg ggtggagcag ggctattttt cgtttcgctc
      781 ggggttcgag gcggctgagc gcctgttgac gggcgacgac cggcccaccg ccgtgttcgc
      841 cagcaatgac gacatggcgc tgggcgtcat ggcggtcgcc aaccgcttgc ggctcaatgt
      901 gccggaaacc ctgtccgtcg ccgggttcga cgacacgccg ggcgccaagg tggtctggcc
      961 ccagcttacc actgttcgcc agcccatcgc cgcgatggcg gcggcggcgg ccgacattct
     1021 gttgagcggc gtatcctggg aggagggcgc gcttcccccg tccaagctgc tggatttcga
     1081 gctggtggtt cgcgagtcca ccgggcccgc cgtgacatag gcttgccaga gatggggagc
     1141 agttcgttgt tgacagcgct gtctcgttgc gctgtcatcc cgccaagcaa gaacgtcgcc
     1201 ctcaagcggc gtctggagga gacaccatct cgttgcccat ttcgcctgac cgcgttcatc
     1261 gacggtcacc gcctccagcc gggggcgtcc aggcgcgtgc ttctcgacgc ggcgcacctg
     1321 cgcgctgcgt cgagcctttt ctgttcgccg gatcgccggc gccgcaccac gggaagtttc
     1381 atggccgctt atcgcccttt cgcccaagcc tcggttctcg cgctcctcat ggccggaagt
     1441 ctcggagcgg cggcgttcgc ccagacgccg tcggcggtcg ccaatcctga actctggccc
     1501 aaggccgcca gc
//
LOCUS       DAOMEO010000894         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8512_length_1511_cov_2.510302, whole genome shotgun sequence.
ACCESSION   DAOMEO010000894 DAOMEO010000000
VERSION     DAOMEO010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8512_length_1511_cov_2.510302"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1511
                     /locus_tag="PLH31_16615"
     CDS             <1..>1511
                     /locus_tag="PLH31_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQR90852.1"
                     /translation="GGTAWRERLSADLADDAAALALIAAYKDIDLAELMTELGGHCLE
                     TLLEAFERASHDDAPRFFIAYTVKGLRLPFQGHKDNHAGLMTPTQIDELRQRLGVVEG
                     EEWDPLSGLSSAERTALKGLIARAPFAADVQRQHVAPIIVTPSADELLEAVAGAKGGE
                     NEQSTQAAFGKVMFEISRRTDEFSGRVVTTSPDVTVSTNLGGFVNRRGVFHRRAHEDV
                     FKRRRIPSAQVWSMVETGQHVELGIAENNLFIALAALGLTAPLFGERLFPVGTLYDPF
                     IARGLDALNYACYQDARFLLVATPSGLTLAPEGGAHQSINGPVIGMAQPGLDTYEPAF
                     ADETAVLMAHAFDRIQAPDGASTYLRLSTRVIDQPERMDDAWRQGTIDGGYWLKAPAP
                     GAKLALVFSGALAPEALAAFEALSEDMPEVGLLAVTSPDVLHRDWTRSGRARWTGKGA
                     RNAPCGSTIETLLSTLDRDAGLVTLADGSPLALSWLGSVRGQRVRALGLETFG"
BASE COUNT          224 a          506 c          526 g          255 t
ORIGIN      
        1 gggcggcacg gcctggcgcg agcgcctgtc agccgatctg gcggacgacg ccgcagccct
       61 ggccctgatc gccgcctaca aggacatcga tctcgccgag ctgatgaccg agctgggcgg
      121 ccactgcttg gaaaccctgc tggaggcctt cgagcgggcc agccacgacg atgcgccacg
      181 gttcttcatc gcctacaccg tcaagggcct gcggctgccg ttccaggggc acaaggacaa
      241 tcacgcgggc ctgatgaccc cgacccagat cgatgagctg cgccagcggc tgggcgtcgt
      301 cgagggcgag gaatgggatc cgctgtcggg cctgtccagc gccgagcgca ccgccctgaa
      361 gggcctgatc gcccgcgcgc cgttcgccgc cgatgttcag cgccagcacg tcgcgccgat
      421 catcgtgacg ccgagcgctg acgaactact ggaggctgtt gcgggcgcca aggggggaga
      481 gaacgagcaa tccacccagg ccgcgttcgg caaggtgatg ttcgagattt cccggcgcac
      541 cgacgagttc tccggccggg tggtcaccac ttcgccggac gtcaccgttt cgaccaatct
      601 cggcggcttt gtgaaccgtc gcggggtctt ccaccgtcgc gcccacgaag atgtcttcaa
      661 gcgccgccgt attccctcgg cccaggtctg gtcgatggtc gagaccggcc agcacgtcga
      721 gttgggcatt gcggagaaca acctgttcat cgccctggcg gcgctgggcc tgaccgcgcc
      781 gctgtttggc gagcgactgt tcccggtggg cacgctgtac gatccgttca tcgcccgcgg
      841 cctcgatgcg ctgaactacg cctgctatca ggacgcccgg tttctgctgg tcgctacgcc
      901 tagcggtctg accctggccc cggagggcgg cgcgcaccag tcgatcaacg ggccggtgat
      961 cggcatggcc cagccggggc tggacactta cgagccggct ttcgcggatg aaacagcggt
     1021 gctgatggcc catgccttcg atcgtatcca ggctcccgat ggcgcctcga cctatctgcg
     1081 gctatcgacc cgggtgatcg accagccgga gcggatggac gacgcctggc gccaaggaac
     1141 catcgatggc ggctattggc tgaaggcgcc ggctcctggc gcaaagctgg ccctggtgtt
     1201 cagcggcgcc ctggcgccag aagccctggc ggcgtttgag gcgctgtcgg aggacatgcc
     1261 cgaggtcggg ctgctggctg tcacctcgcc cgatgtcctg caccgcgact ggacgcgtag
     1321 cgggcgtgcg cgctggacag ggaagggggc gcggaatgcc ccttgtggct cgaccatcga
     1381 aaccttgctg tcgacgctcg accgcgacgc gggccttgtt accctggcgg acggttcgcc
     1441 gctggcgctg tcgtggctgg gctcggtgcg tgggcagcgc gtgagggccc tgggcctgga
     1501 aacctttggc c
//
LOCUS       DAOMEO010000895         1511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8516_length_1511_cov_1.660027, whole genome shotgun sequence.
ACCESSION   DAOMEO010000895 DAOMEO010000000
VERSION     DAOMEO010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8516_length_1511_cov_1.660027"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLH31_16620"
     CDS             complement(<1..897)
                     /locus_tag="PLH31_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR90853.1"
                     /translation="MTGAVTLPPAKSRPARRRGHEFAFLPAALEVVETPPSPIGRAIS
                     GLIIVVFLVAILWASLGRIDIIAVAQGKIIPSGSTKTLQPLESGIVRAIHVKDGQAVK
                     AGDVLVELDSTVSVAERTRTEADLVAARLDAARLRALLSDVPDPVAQFEPPQEADRAL
                     VAAQRHLLASEVAERRAKAAAMDNQKIQKEKEVATLAASIAKIEAILPILRQRFEIRR
                     ALMEKDLGSRLLYLQEQGELAGTENELLIQKSRHAEAVAALGAITATRDQVEAEFRRK
                     TAEELSKAEQRVAALTQDLVKTT"
     gene            complement(894..>1511)
                     /locus_tag="PLH31_16625"
     CDS             complement(894..>1511)
                     /locus_tag="PLH31_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR90854.1"
                     /translation="KLIQRLYVPESGRVLIDGVDLALVDTSWLRRQVGFVLQENVLLN
                     RTVHENIALTDPAMPMEKVISAAKLAGAHEFILELPEGYDTIVGERGGSLSGGQRQRL
                     AIARALVTDPRILIFDEATSALDYESERIIQENMAEIARGRTVFIIAHRLSAVRHADR
                     IVTMDHGRIVEDGKHEDLVRIGGRYAQLYRLQGGIQVVSEAGRDR"
BASE COUNT          227 a          518 c          503 g          263 t
ORIGIN      
        1 ggtggttttc acgagatcct gcgttagcgc agcgacgcgc tgctcggcct tcgacagttc
       61 ctcggcggtc ttgcgccgga actcggcctc cacctggtca cgggtggccg tgatggcccc
      121 cagggcggcg acggcttcgg catggcggct tttctggatc aacagctcgt tctcggtgcc
      181 ggccaattcg ccctgctcct gcaggtagag cagcctggaa ccgagatcct tctccatcag
      241 cgcccgccgg atctcaaagc gctggcgcag gatcgggagg atcgcctcga tcttggcaat
      301 gctggccgcg agcgtcgcga cctctttctc cttctggatc ttctggttgt ccatcgccgc
      361 ggccttggcg cgccgctccg cgacctcgct ggcgaggaga tggcgctgag cggccaccag
      421 cgcccggtcc gcttcctggg gcggctcgaa ctgcgcgacc ggatcgggca cgtccgagag
      481 aagggcacgc agccgcgccg cgtcgaggcg cgccgccacc agatcggcct cggtgcgcgt
      541 ccgctcggcg acactgacgg tcgaatcaag ctcgaccagt acgtcgcccg ccttgaccgc
      601 ctgaccatcc ttgacgtgga tcgcccttac gatgccgctc tccagcggct ggagggtctt
      661 ggtactgccg ctcgggatga tcttgccctg cgcgacggcg atgatgtcga tccggccgag
      721 agacgcccac aggatcgcga cgaggaagac cacaatgatg aggccgctga tcgcccggcc
      781 gatcggcgat ggcggcgtct cgacgacctc gagcgccgcc ggcaggaacg cgaactcgtg
      841 gccacgacgc cgggcgggcc gggacttggc cggcggcagg gtaacggccc ccgtcatcgg
      901 tctcgcccag cctcggagac gacctggatg ccgccctgca agcgatagag ctgggcgtag
      961 cgaccaccga tccgcaccag gtcctcgtgc ttgccgtcct cgacgatccg gccatggtcc
     1021 atagtgacga tgcgatcggc gtgccggacg gccgacaatc ggtgcgcgat gatgaacacg
     1081 gtccgccccc gggcgatctc ggccatgttc tcctggatga tgcgttcgct ctcgtagtcg
     1141 agcgcgctgg tcgcctcgtc gaagatgagg atgcgcgggt cggtcacgag ggcccgggca
     1201 atggccagcc gctggcgctg tccgccggac aggctgccgc ccctctcgcc cacgatcgtg
     1261 tcgtagcctt ccggcaattc caggatgaat tcgtgagccc cggccagctt cgccgccgag
     1321 atcaccttct ccatgggcat cgccgggtcg gtcagggcga tgttctcatg caccgtgcgg
     1381 ttgagcagaa cgttctcctg cagcacgaag ccgacctgcc ggcgcaacca ggaggtgtcg
     1441 acgagcgcaa ggtcgacgcc atcgatcagg acgcggccgc tttccgggac gtagagtctc
     1501 tggatcagct t
//
LOCUS       DAOMEO010000896         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8518_length_1510_cov_2.804124, whole genome shotgun sequence.
ACCESSION   DAOMEO010000896 DAOMEO010000000
VERSION     DAOMEO010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8518_length_1510_cov_2.804124"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLH31_16630"
     CDS             complement(<1..1100)
                     /locus_tag="PLH31_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR90855.1"
                     /translation="MFRTLLTAAAVSALMVGSAFAQDAATAEALRDKALRDHLAWDLT
                     QELTTTIGPRLVGSPAMEKAKDWGVAKLKALGFTNIKVEQFAKPSWTRGEESAWLMAP
                     YAMNLNPLGLGRTVPTPAGGVEAEVALFATYAEMIAAPEGALKGKIVVITQPMVRAQD
                     GAGYGVAGISRRAGPVEAAKRGAIALLIRSISTSDSSVPHTGVTTHGDDVVTIPAAAI
                     GVPEAEQLQRLAAKGPLRIKLNLQSSVDPKGVAWNISGDIKGSEKPDEVIVIGGHLDS
                     WDVGTGALDDATGVAITTAAAKLIGDLPKHPRRTIRVVMWGSEESGGSSDAYLAANKD
                     SLSRIILAGESDSGADRIYSLKVPAGSLEHPV"
     gene            complement(1348..>1510)
                     /locus_tag="PLH31_16635"
     CDS             complement(1348..>1510)
                     /locus_tag="PLH31_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HQR90856.1"
                     /translation="GAMFSPLHSNFLINTGEATAADLEGLGDAVRADVLAKTGIQLDW
                     EIKRIGRPA"
BASE COUNT          231 a          510 c          519 g          250 t
ORIGIN      
        1 atcaccggat gttccagcga gccggccgga accttcaggc tgtagatgcg gtcggcgccg
       61 ctgtcgctct cgcccgccag gatgatcctg gacaggctgt ccttgttggc ggccagatag
      121 gcgtcggacg agccgccgct ttcctccgag ccccacatca cgacgcggat ggtgcgcctg
      181 gggtgcttgg gcaggtcgcc gatcagcttg gcggcggccg tggtgatggc gacgcccgtg
      241 gcgtcgtcca gagcgccggt gccgacgtcc cagctgtcga gatggccgcc gatgacgatc
      301 acctcgtccg gcttttccga gcccttgata tcgcccgaga tgttccacgc cacgcccttg
      361 gggtcgacgc tggactgcag gttcagcttg atgcgcagcg ggcccttggc ggccaggcgc
      421 tgaagctgct cggcttccgg cacgccgatc gcggcggcgg gaatggtcac cacgtcgtcg
      481 ccatgggtgg tgacgccggt gtggggcacc gaggagtccg aggtcgagat cgagcggatc
      541 agcagggcga tggcgccgcg cttggcggcc tccacgggac cggcccggcg cgaaatgccg
      601 gccacgccat agccagcgcc gtcctgggcg cgaaccatcg gctgggtgat gaccacgatc
      661 ttgcccttca gcgcgccttc cggcgcggcg atcatctcgg cataggtcgc aaacagcgcc
      721 acctcggcct cgacgccgcc ggccggcgtc ggcacggtgc ggcccaggcc cagggggtta
      781 aggttcatcg catagggggc catcagccag gcgctctcct cgccgcgcgt ccaggacggc
      841 ttggcgaatt gctcgacctt gatgttggtg aagcccaggg ccttcagctt ggccacgccc
      901 cagtccttgg ccttttccat cgctggcgag cccaccaggc gggggccgat cgtggtggtc
      961 agctcctgcg tgagatccca ggccaggtgg tcccgcaggg ccttgtcacg cagcgcctcg
     1021 gcggtggcgg cgtcctgggc gaaggcagag ccgaccatca gggcggaaac ggcggcggcg
     1081 gtcagcaggg tgcggaacat aggcgaggag tctcccgaaa tttggccgat ctcttagcac
     1141 cggcgcccgc ccgaaacagg caacaaccgg atgaactatc ggtaaccttc ggttcaaccg
     1201 gctataggct gcgcagctca aacaagctgt catcccggac aagcgtgcag cgcgccgatc
     1261 cgggaccgcc ggaagcgcgg cgctcaggcg tcggtcccgg atcttctctt cgctacgtcc
     1321 gggatgacag tcgttagcat gcgcgcccta agccggccgc ccgatgcgct tgatttccca
     1381 gtccagctgg atgccggtct tggccagcac gtcggcgcga accgcatcgc ccaggccctc
     1441 cagatcggcc gccgtggcct cgccggtatt gatcaggaag ttcgaatgca gcggcgagaa
     1501 catcgccccg
//
LOCUS       DAOMEO010000897         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8523_length_1509_cov_2.349381, whole genome shotgun sequence.
ACCESSION   DAOMEO010000897 DAOMEO010000000
VERSION     DAOMEO010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8523_length_1509_cov_2.349381"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLH31_16640"
     CDS             <1..734
                     /locus_tag="PLH31_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HQR90857.1"
                     /translation="VLDPAGRLVGLTDGKLEGLKGPDGQPLQDKDKADAEGLAILANG
                     DRLISFERDHRIWLYPAGGGAPRPAPKPATTLPYNEGIEALAAYPAAGPDAYLAGGEE
                     GEVWLCKLSAACQALAPQQGPDFTWGLTAMASFDGPAIATLHRGFDPIRGWRGILRFV
                     ANPLTPAARQVHAASLHLDGSVTRDNYEGVALSRTPSGATRIYLISDDGGLPGQRTLL
                     MAFDWTAPPPPPPPPAKTRKPVRKR"
     gene            911..1216
                     /locus_tag="PLH31_16645"
     CDS             911..1216
                     /locus_tag="PLH31_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90858.1"
                     /translation="MIRLLVLAVSALMTLSAGAASAAELTVKVAGKSTPQLHADLVNA
                     ATTICREDLDRTTFAADLMPYCVREVTRAAIDKVRSPALAAYDKAQGRSAYLVKISR"
     gene            1304..>1509
                     /locus_tag="PLH31_16650"
     CDS             1304..>1509
                     /locus_tag="PLH31_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90859.1"
                     /translation="MRRNIIVDNPPCCGYGFFDGQTGKNRTDVRGTRREEVEPNPLSW
                     TTNMIRTIALAVSAIALTVAAPVSA"
BASE COUNT          253 a          554 c          455 g          247 t
ORIGIN      
        1 tggtgctgga cccggccggt cggctggtcg gcctgacgga tggcaagctt gagggcctca
       61 agggccccga tggccagccc ttacaggaca aggacaaggc cgacgccgag ggcctggcca
      121 ttctggccaa tggcgatcgc ctgatcagct ttgagcgcga ccaccgtatc tggctctatc
      181 cggcgggcgg cggcgccccg cgtccggcgc ccaagcccgc gacgaccctg ccctataacg
      241 aaggcattga ggccctcgcc gcctatccgg ccgctggtcc cgacgcctat ctggcgggcg
      301 gcgaggaggg cgaggtctgg ctttgcaagc tgtcggccgc ctgccaggcc ctggccccgc
      361 agcagggccc tgacttcacc tggggcctga cagccatggc ctcgtttgac ggaccggcca
      421 tcgccacgct gcatcgcggc ttcgacccca tccggggctg gcgcggcatc ctgcggttcg
      481 tggccaatcc cctgacgccg gccgccagac aggtccacgc cgccagcctg cacctggacg
      541 gatccgtcac ccgcgacaac tacgagggcg tggcgctcag ccgtacgccc tccggcgcga
      601 cgcggatcta cctgatctct gacgacggcg gcctgccggg ccagcgcacc ctgctgatgg
      661 cgttcgactg gaccgccccg ccccctccgc cgcctccgcc ggccaagacc cgcaagcccg
      721 ttcgcaaacg ctgagcccgg cctgcgacaa gttaccgcta acattttcat gttgcgacgc
      781 agcatcgcgc ttgcggtgac aacgttgtca tgtattcttg gtccgacggt tgacgaggcg
      841 agacccacag gccgtcagag gaacgcgtcg ggaggaggtt gaacccaacc ccctttccgg
      901 gactaaaatc atgatccgtc tgcttgttct ggccgtatcg gccctgatga ccctctccgc
      961 cggcgccgcc agcgccgccg aactgaccgt aaaggtcgct ggcaagtcca cgccccagct
     1021 ccacgccgac ctggtgaacg ccgcgaccac catctgccgc gaagatctgg accgcacgac
     1081 cttcgccgcc gacctgatgc cctactgcgt ccgtgaagtg acccgcgccg ccatcgacaa
     1141 ggttcgcagc ccggctctgg ccgcctatga caaggctcag ggccgctctg cctatctggt
     1201 gaagatcagc cgctaaccac ccccctccat cgccggacaa gcgccccgcg aagcctcgct
     1261 tcgcggggcg cttgatggtc gagcacgcgc caaaaaaatc tgggtgcggc gcaacataat
     1321 cgttgataac ccaccttgct gcggctatgg cttttttgac ggccaaacag gcaagaaccg
     1381 gacggacgtc agaggaacgc gtcgggagga ggttgaaccc aacccccttt cgtggactac
     1441 gaacatgatc cgcaccatcg ccctggccgt ttcggccatc gccctgactg tcgctgctcc
     1501 cgtttcggc
//
LOCUS       DAOMEO010000898         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8524_length_1509_cov_2.114168, whole genome shotgun sequence.
ACCESSION   DAOMEO010000898 DAOMEO010000000
VERSION     DAOMEO010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_1509_cov_2.114168"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="ilvN"
                     /locus_tag="PLH31_16655"
     CDS             complement(<1..393)
                     /gene="ilvN"
                     /locus_tag="PLH31_16655"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQR90860.1"
                     /translation="MTNAPAASGSAYDMPVASEAEQTATFALLVDNEPGVLHRVVGLF
                     AARGYNIESLTVAETDHKAHTSRITVVARGTRHVLDQIEAQLQKVVSVRRVHDVTRDP
                     NGVERELALVKVRGSGTDRVEALRVADIF"
     gene            complement(393..680)
                     /locus_tag="PLH31_16660"
     CDS             complement(393..680)
                     /locus_tag="PLH31_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HQR90861.1"
                     /translation="MNKTTLSSEQVERLAKLDALPDDKIDTVGIPEAPAENALLARRP
                     GLGEPRAHPDAVALDADVVGWFKEHAGDKPYQTEINRVLRQHVAKTEKKRA"
     gene            complement(661..951)
                     /locus_tag="PLH31_16665"
     CDS             complement(661..951)
                     /locus_tag="PLH31_16665"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HQR90862.1"
                     /translation="MVIFEWGAQKARSNPANHGVAFDDAKLVWADPLNVVYPDRFIDG
                     EQRWHAVGVVGFVTVLLVVHIYIGGTDAERVRIISARKATPWERKIYEQDNA"
     gene            complement(991..>1509)
                     /locus_tag="PLH31_16670"
     CDS             complement(991..>1509)
                     /locus_tag="PLH31_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR90863.1"
                     /translation="NRWMTSGGLGTMGYGLPAAVGVQMAHPDALVIDIAGDASVQMTM
                     QEIATAIQYDLPVKIFIINNEWMGMVRQWQQLLHGERYSHSYSDSLPDFVKLAEAYGA
                     VGIRCSDPAELDGKILEMINSDKVVVFDCRVEKHENCLPMIPSGKAHNEMIMGDVEDI
                     GNVIGAAGAGLV"
BASE COUNT          253 a          483 c          469 g          304 t
ORIGIN      
        1 gaagatgtcg gccacgcgca gggcctcgac ccggtcggtg cccgaaccgc gcaccttgac
       61 cagggccagc tcgcgctcga cgccgttggg atcgcgggtc acgtcgtgga cgcggcggac
      121 gctgaccacc ttctgaagct gggcctcgat ctggtccagt acatgccgcg tgccgcgcgc
      181 caccacggtg atgcggctgg tgtgagcctt gtggtcggtc tcggcgaccg tcaggctctc
      241 gatattgtag ccgcgcgcgg cgaacagccc gaccacgcga tgcagcacgc ctggttcgtt
      301 atccaccagc agagcgaagg tcgccgtctg ctcggcttcg gaggcgacgg gcatgtcgta
      361 ggcggatccg gaggcggcgg gggcgttggt catcaggcgc gcttcttttc tgtcttggcc
      421 acgtgctggc gcagcacgcg gttgatctcg gtctgatagg gcttgtcgcc cgcatgttcc
      481 ttgaaccagc cgaccacatc ggcgtccagc gccacggcgt caggatgtgc gcgcggctcg
      541 ccaaggccag gccgtcgcgc caggagggcg ttctccgcag gggcttcggg aatacccact
      601 gtatcgatct tatcgtcggg cagggcgtcg agttttgcca gacgctctac ctgctcagaa
      661 ctaagcgttg tcttgttcat agattttgcg ctcccaagga gtcgctttcc gcgcgctgat
      721 gatccgaact cgttccgcat ccgtgccacc aatatagatg tgaaccacca gcaagaccgt
      781 aacaaatccg acaacaccga ccgcgtgcca tcgttgctca ccgtcgatga accggtccgg
      841 gtaaaccacg ttgagcggat cggcccagac cagttttgcg tcgtcaaaag ctacgccgtg
      901 gttggccgga ttagatcttg ccttctgcgc gccccattcg aatatcacca tgacgggtat
      961 ttgttgaaat acccgtcatg gctgtaaacc ttaaaccagt cccgccccag ccgcgccgat
     1021 gacgtttccg atgtcctcga cgtcgcccat gatcatctcg ttgtgggcct tgcccgaggg
     1081 gatcatcggc aggcagttct catgcttctc gacgcggcag tcgaagacca cgaccttgtc
     1141 gctgttgatc atttccagga tcttgccgtc cagctcggcc ggatccgagc agcggatgcc
     1201 gacggcgcca taggcctcgg ccagcttcac aaagtcgggc aggctgtcgg aataggagtg
     1261 gctgtagcgc tcgccgtgca gcagctgctg ccactggcgg accatgccca tccactcgtt
     1321 attgatgatg aagatcttga ccggcaggtc gtactgaatg gccgtggcga tctcctgcat
     1381 ggtcatctgc acgctggcgt cgccggcgat gtcgatgacc agcgcgtccg ggtgcgccat
     1441 ctgcacgccc acggcggccg gcaggccgta gcccatggtg cccaggccgc cggaggtcat
     1501 ccagcggtt
//
LOCUS       DAOMEO010000899         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8534_length_1508_cov_1.943565, whole genome shotgun sequence.
ACCESSION   DAOMEO010000899 DAOMEO010000000
VERSION     DAOMEO010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8534_length_1508_cov_1.943565"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLH31_16675"
     CDS             complement(<1..188)
                     /locus_tag="PLH31_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR90864.1"
                     /translation="MRVRLAAALVVALLPVLLLGGLQSLFAFRAEADLRRQSLELAAG
                     RSAAIAHARIAAAGVLLQT"
     gene            complement(227..838)
                     /locus_tag="PLH31_16680"
     CDS             complement(227..838)
                     /locus_tag="PLH31_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR90865.1"
                     /translation="MTDTEAQVGRSPFDPPRDNAFKRELVTLIPHLRAFARTLTGDPT
                     AADDLAQDAMMKAWDARASFQLGTNMKAWTFMILRNQFYSEKRRSWRQSQLDQEAAER
                     TLVAVDDPEAPVALDELRMSLGMLPAEQREALILVGAGGFAYEEAAVICGCAVGTVKS
                     RVSRARRALQAILEAGAYDRDGRSAGDAMGSILADAERLSSLR"
     gene            complement(841..1026)
                     /locus_tag="PLH31_16685"
     CDS             complement(841..1026)
                     /locus_tag="PLH31_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HQR90866.1"
                     /translation="MIEHVPMDDHRKGAAALDEARLRQQAIGVKLRQLFDEVVNEQVP
                     DEFLEILRKAEKPAGEE"
     assembly_gap    1213..1285
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1297..>1508
                     /locus_tag="PLH31_16690"
     CDS             1297..>1508
                     /locus_tag="PLH31_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR90867.1"
                     /translation="MSLLARLAPHLPYIRRYARALTGDQNTGDHYVRVALEALAAGER
                     SLDADLSPRVALYRVFHAIWLSTGAQL"
BASE COUNT          243 a          481 c          454 g          257 t
ORIGIN      
        1 gtctgcagaa gaacccctgc cgccgcgatg cgcgcatggg cgatggccgc gctgcgaccc
       61 gccgccagct caagactctg gcggcgcagg tccgcctcgg cgcggaacgc aaacagcgac
      121 tgcaggccgc ccagcaacag gaccggcagg agggcgacga ccagcgccgc cgccaggcgg
      181 acgcggatcg aggttacagc ctccgcgccc cggcttttga aaggtctcac cttaaggagc
      241 tcaatcgctc cgcgtcggcc agaatcgaac ccatcgcatc gcccgccgat ctgccatccc
      301 ggtcataggc gccggcttcc aggatcgcct gcagcgcccg acgggcgcgg ctgacgcggc
      361 ttttgacggt gcccacggcg cagccgcaga tcactgcggc ttcctcatag gcaaagccgc
      421 cagcgccgac gagaatgagg gcttcacgct gttcggcggg aagcatgccc aggctcatgc
      481 gcagttcgtc cagcgccacg ggcgcctcgg gatcatcgac cgcgaccagg gtccgctcgg
      541 cggcctcctg atcaagctgg ctctgacgcc aggagcggcg tttttccgaa tagaactggt
      601 tgcgcaggat catgaaggtc caggccttca tgttcgtgcc aagctggaag ctggcgcggg
      661 cgtcccaggc cttcatcatg gcgtcctgcg ccagatcgtc ggccgcggtg ggatcaccgg
      721 tcagggtgcg cgcaaaggcg cgcagatgcg ggatcagggt caccagctcg cgcttgaaag
      781 cattgtcgcg cggcgggtcg aacggcgagc ggccgacctg agcttcggtg tccgtcatgc
      841 tcattcctcg cctgcaggct tctcggcctt gcgcagaatt tcgagaaatt cgtcgggaac
      901 ctgctcgttg acaacctcgt cgaacagctg gcgcagcttc acgccaatag cctgctgacg
      961 caggcgagct tcgtcgagcg ccgcagcgcc tttgcggtga tcatccatgg gtacgtgttc
     1021 gatcatgtcc tcgacgccgg tgtaacgcag cgcccgcccc ctttggccga atgagtgtct
     1081 gccagacgac aacgcggttt tccgtatcgg gttccacaag cttgggcata agatgccaaa
     1141 attccgcgtg gggcggaacc cgctggataa ggcggcgttt aagaccctga tcgaatggcc
     1201 cagcttggcc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnncggga ggggtcatga gtcttcttgc tcggctggcg
     1321 ccacatctac cttatatccg tcgctacgcc cgcgccctta ctggcgatca gaatactggc
     1381 gaccattatg tccgggtcgc cctggaagcg ctggccgccg gggagcgttc gctggacgcc
     1441 gacctgtcgc cccgcgtcgc gctctatcgc gttttccatg ccatctggct ttccacaggc
     1501 gcccaact
//
LOCUS       DAOMEO010000900         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8540_length_1506_cov_2.271537, whole genome shotgun sequence.
ACCESSION   DAOMEO010000900 DAOMEO010000000
VERSION     DAOMEO010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8540_length_1506_cov_2.271537"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLH31_16695"
     CDS             <1..254
                     /locus_tag="PLH31_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HQR90868.1"
                     /translation="LVYPALLFSGACILSKRLHDRGRSGWFAALILVAIVAVWPQPEH
                     FLDFVFSIVILWAVVELGVMGSEQGANRYGPNPLKSISI"
     gene            complement(251..976)
                     /gene="lipB"
                     /locus_tag="PLH31_16700"
     CDS             complement(251..976)
                     /gene="lipB"
                     /locus_tag="PLH31_16700"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517631.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQR90869.1"
                     /translation="MLDRPLSLENKGNPLFGRGDGAAVGWAVSTGYVPYPAAVAAMEA
                     RAAAIADGTAGELVWLLEHPPLYTGGVSAKGADLIEPDRFPVFASGRGGQYTYHGPGQ
                     RVAYVMLDLTKRDRDVRAFVQGLEAWIIDALAAFNVVGEMREGRVGVWVERKGAGWSR
                     EDKIAAIGVKLRKWVSFHGISLNVEPDLDHFSGIVPCGQTEHGVTSLVDLGLPVTMDE
                     ADAALKASFQRVFGPVEDAEAPV"
     gene            1047..1298
                     /locus_tag="PLH31_16705"
     CDS             1047..1298
                     /locus_tag="PLH31_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HQR90870.1"
                     /translation="MSQVNAVNVEISVLLGRSILPMQQLLRMGRGAVIPLDAKTNDEV
                     WILANNHPIARGEIQISDDRIAIQVTRAADVYDYMAGGV"
     gene            1382..1468
                     /locus_tag="PLH31_16710"
     tRNA            1382..1468
                     /locus_tag="PLH31_16710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1416..1418,aa:Leu,seq:gag)
BASE COUNT          245 a          508 c          470 g          283 t
ORIGIN      
        1 ggctggtcta tccggccctg ctgttttcgg gcgcatgcat cctgtccaag cggctgcacg
       61 acaggggccg gtcgggctgg ttcgccgcct tgatcctggt ggcgatcgtc gccgtctggc
      121 cccagcccga gcacttcctc gacttcgtgt tctcgatcgt catcctgtgg gcggtcgttg
      181 agctgggcgt gatgggcagc gagcaggggg ccaatcgcta tgggcccaat cccctgaagt
      241 cgatctcgat ctagaccggg gcctctgcgt cttcgaccgg cccgaacacc cgctggaagc
      301 tggctttcag cgccgcgtct gcttcgtcca tggtcaccgg caggcccaga tccacgaggc
      361 tggtgacgcc atgctcggtc tggccgcacg gcacgatgcc tgagaagtgg tcaagatccg
      421 gctcaacgtt caggctgatc ccgtggaagc tgacccactt gcgcagcttg accccgatgg
      481 cggcgatctt gtcctcgcgc gaccagcccg cgcccttgcg ctcgacccac acgccgaccc
      541 ggccctcgcg catttcgccg accacgttga aggcggccag ggcatcgatg atccaggctt
      601 ccaggccttg tacgaaggcg cgcacatcgc ggtcgcgctt cgtcaggtcc agcatcacat
      661 aggccacccg ctggccgggg ccgtgatagg tgtattgccc gccgcgcccg ctggcgaaca
      721 ccgggaagcg atccggctcg atcaggtccg cgccctttgc cgagacgccg ccggtataga
      781 gcggcggatg ctccagcagc cagacaagct cgcccgctgt cccgtcggcg atggcggcgg
      841 cgcgggcctc catggcggca acggctgccg ggtaggggac gtaaccggtc gaaaccgccc
      901 accccacagc cgcgccgtcg ccgcgcccaa acagcgggtt tcctttattt tcaaggctta
      961 acgggcgatc aagcataagg ccctattctt gcaatctcga tggcgcgacc agtctggtcg
     1021 agagtcgatt ttaggggcct tagttcgtga gccaggtcaa cgccgtcaat gtcgagatct
     1081 ccgttctttt ggggcgctcg attctaccca tgcagcagct gctgcgcatg ggccgcggcg
     1141 cggtgatccc gctggacgcc aagaccaatg acgaggtctg gatcctggcc aacaaccacc
     1201 cgatcgcccg gggcgagatc cagatcagcg acgaccgcat cgccatccag gtgacgcgcg
     1261 ccgccgacgt ctatgactac atggccggcg gcgtctgacg ccgtcccaga tagttcgctt
     1321 tccggcaact ctgccctttc caaaccagat tcagtttgct acctgccgcc cctcaccacg
     1381 tgcggtcgtg gcggaattgg tagacgcgca gcgttgaggt cgctgtgccc gaaagggcgt
     1441 ggaagttcga gtcttctcga ccgcaccagg tgagacctac tagagcgtgc ctggcgaaat
     1501 cggttt
//
LOCUS       DAOMEO010000901         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8546_length_1505_cov_1.987586, whole genome shotgun sequence.
ACCESSION   DAOMEO010000901 DAOMEO010000000
VERSION     DAOMEO010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8546_length_1505_cov_1.987586"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLH31_16715"
     CDS             <1..513
                     /locus_tag="PLH31_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90871.1"
                     /translation="PLPTLYSKYRGTGFPLRNNNRRLLPPWRELSPWMKLQVATLVLA
                     EGSFIQFTVHLHDDRREELEAQGADLRKYILDRVTRRLGALSLGYVGEPKAYRNWVAH
                     NIRDVLLNAFATKPRIGRIPQIKNACVCVVHVTQTFQPDYGSGDLANAIAERNDLPAE
                     LVPFLKLTLA"
     gene            977..1282
                     /locus_tag="PLH31_16720"
     CDS             977..1282
                     /locus_tag="PLH31_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90872.1"
                     /translation="MLPRSRICNYQTGERWACGQRASIALLNVAGATTIDCRPKDPDR
                     ASVVICRLAGSDLAELRPREGWGALAGGVTEKRYIDVAAAAFANKNGMWMLRPPPRR"
BASE COUNT          311 a          444 c          462 g          288 t
ORIGIN      
        1 ccactaccta cattatatag taagtacaga ggaacaggat tccctctacg aaacaacaat
       61 aggcgcttac ttcctccatg gcgcgaactg tctccctgga tgaagctaca agttgccact
      121 ctggtattag cggaggggag cttcatccaa tttactgttc accttcatga tgaccgccga
      181 gaggagttag aggctcaggg agcagattta cggaagtata tattggatcg cgtaacccga
      241 agactaggcg cgctgtcgtt gggttacgtc ggggagccca aggcctatag gaattgggtg
      301 gcgcacaaca tccgagatgt tctgctgaac gcttttgcga ccaaacctcg gataggtcga
      361 ataccccaaa ttaagaatgc ctgtgtatgt gtcgtgcatg ttacgcaaac tttccagcct
      421 gattatggtt ctggcgatct ggccaacgcc attgcggagc gcaacgacct gccggcggag
      481 ttggtgccgt ttctgaagtt gaccttggcc tgaagcgatt ttccggaaat tgaatacgcc
      541 gactattggc cgcgcccagg tccgagcgca caggacggtc tgtcggttcc atgatctggc
      601 ttaccgggtg tcacgcatcg cagccaggac cgcgagcacg ccagacgccc aataattccg
      661 accctttcaa gttttgccgc agtgctttcc aaccgcgtga ttcggctatg gctttggaag
      721 gttaagaatg gccgcgttaa tctcaccgtt tgcgaataac gggacgcttt cggctgtcca
      781 aggcacccag gatgcgtaat ggctcgagac gccaaccgcc gccccttaac cgacccacgc
      841 cgcgggtcgc gcagcgcacc gtcggtgggg caacgcaggc ggctggacgg cgctagagcc
      901 cgccttcaag aattaccagg ttcagatcaa taacgatggc ggcctacaag cgggcgatcg
      961 aactcatcgc gtcaagatgt tgccgcgcag ccgcatatgc aattatcaaa ccggcgagcg
     1021 ctgggcctgc ggccaacgcg cctctatcgc cttgttgaac gtggcgggcg cgacgaccat
     1081 cgattgccgg ccgaaagatc cggatcgggc cagcgtcgtg atctgccgcc tggccggctc
     1141 cgacctcgcc gaactgaggc cgcgcgaagg ctggggggcg ctggcgggcg gcgtcacaga
     1201 gaaacgctat atcgacgtgg cggccgcagc cttcgccaac aagaacggca tgtggatgct
     1261 gcggccaccg ccgcgacgct gattaattgc gataagagac caccggctcg cgcgagcgag
     1321 acggccacgg gaccctttgt cgcggcggcc tcgtccgagc tgtggcgccg cgcgacgcca
     1381 ggcagtgccg gccccggtgg gaggcgcgga aagcctcggc cgtcttagcc ggggcaggct
     1441 ggcccttcca tttgacccgg cggaacgttt gcgcggaggg caagcggcgt cgaagacaca
     1501 tcggc
//
LOCUS       DAOMEO010000902         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8550_length_1504_cov_3.107660, whole genome shotgun sequence.
ACCESSION   DAOMEO010000902 DAOMEO010000000
VERSION     DAOMEO010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8550_length_1504_cov_3.107660"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1504
                     /locus_tag="PLH31_16725"
     CDS             <1..>1504
                     /locus_tag="PLH31_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQR90873.1"
                     /translation="YDLTATTPSNTQTVRDVLAGDVWLCSGQSNMEQQVLASSDAWNQ
                     INNVTPNDTIRMVTIEKADGRAPKANFAKPPVWKPTTKDTVGSFSASCFYFARELQKT
                     RQVPLGLVHASWGGSGIEAWMTPAALRKVGGYDEAIDILNLSTTDPAAASRRWGETIK
                     GWWTSKHPGEAAIAPWTGAGPWTPAPRDLGVWERWGVAPLAAFNGVVWFKTEVTLTAA
                     QARQAATLNLGQVDEMDTTYLNGVGVGATSGAWLDRSYPLAAGALKAGRNTLVIAAND
                     TYGNGGIYGDPSKRAIVLADGTRLPLEAWTYRIEPRVGADPPRAPWDTLAGVSTLYNG
                     MLAPMGPYAYSGLAWYQGETNVGRDQTYAALQTAFVAERRAGMAPDMPVVVTQLASYG
                     AFATTPGAFGTAALREAQRRAVIADPHAGLAVTTDIGDPFDIHPANKQEVGRRLALAA
                     RRIAGEAVTTGPAPVSAKRAGETVVVTFEQIGGALVSRGSDRVIGFELCDA"
BASE COUNT          235 a          553 c          507 g          209 t
ORIGIN      
        1 ctacgacctc accgccacca cgccctcgaa cacccagacc gtccgcgacg tgctggccgg
       61 cgatgtgtgg ctttgctcgg gccagtcgaa catggagcag caggtcctgg cctccagcga
      121 cgcctggaac cagatcaaca acgtcacgcc gaacgacacc atccgcatgg tgaccatcga
      181 gaaggctgac ggccgcgcgc ccaaggccaa cttcgccaag ccgccggtct ggaagcccac
      241 gaccaaggac accgtcggct cgttctcggc cagctgcttc tatttcgccc gcgagctgca
      301 gaagacgcgg caggtcccgc tgggcctggt ccacgcctcg tggggcggat cgggcatcga
      361 ggcctggatg acgccggccg ccctgcgcaa ggtcggcggc tatgacgagg ccatcgacat
      421 cctcaacctg tcgaccaccg accccgccgc cgccagccgt cgctggggcg agacgatcaa
      481 gggctggtgg acgtccaagc atccgggcga ggccgcgatc gcgccgtgga ccggagcagg
      541 cccctggacg cctgcgccca gggacctggg cgtctgggag cgctggggcg tcgcgccgct
      601 ggccgccttc aacggcgtgg tctggttcaa gaccgaggtc acgctgaccg ccgcccaggc
      661 gcggcaggct gcgaccctga acctgggcca ggtcgacgag atggacacca cctatctgaa
      721 cggcgtcggc gtgggggcca cctcgggcgc gtggctggac cggtcctatc ctctggccgc
      781 cggagcgctg aaggcgggca ggaacaccct cgtgatcgcc gccaacgata cctatggtaa
      841 tggcggaata tacggcgatc cctccaaacg cgccatcgtt ctggctgacg gcacgcggct
      901 gccgctggag gcctggacct atcgcattga accccgcgtg ggcgccgatc cgccgcgtgc
      961 gccttgggac accctggccg gggtctcgac cctttataac ggcatgctcg cgcccatggg
     1021 gccttacgcc tattcgggcc ttgcctggta ccagggcgag accaatgtgg gccgcgacca
     1081 gacctatgcg gccctgcaga ccgccttcgt cgccgagcgc cgcgcgggca tggcgcccga
     1141 catgccggtg gtggtgacgc aactggccag ctacggcgcc ttcgccacca cgccgggcgc
     1201 tttcggcaca gccgccctgc gcgaagccca gcgccgcgcg gtcatcgccg acccccatgc
     1261 gggcctggcc gtgaccaccg acatcggcga ccccttcgac atccaccccg ccaacaagca
     1321 ggaggtcggc cgccgcctgg ccctggccgc ccgccgcatc gccggcgagg ccgtgaccac
     1381 ggggccggcg ccagtgtcag ccaagcgggc aggggagacg gtggtcgtca cgttcgagca
     1441 gatcggcggg gccttggtct cgcgcggctc ggaccgggtg atcggcttcg agctctgcga
     1501 cgcc
//
LOCUS       DAOMEO010000903         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8574_length_1500_cov_1.986159, whole genome shotgun sequence.
ACCESSION   DAOMEO010000903 DAOMEO010000000
VERSION     DAOMEO010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8574_length_1500_cov_1.986159"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..168
                     /locus_tag="PLH31_16730"
     CDS             58..168
                     /locus_tag="PLH31_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90874.1"
                     /translation="MVWKLDHLGRSLRQLGDFTSQLEARRTHFRQPDGWH"
     gene            206..499
                     /locus_tag="PLH31_16735"
     CDS             206..499
                     /locus_tag="PLH31_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR90875.1"
                     /translation="MAAMAEMERDLVRERTVAGLAAAKACGRLGGRPSKLTAQQIGHA
                     KRLLADPEISGAEVARTLGVARSTLYRSIERPAGVARKRYRRPSSSLESGTAR"
     gene            complement(1148..>1500)
                     /locus_tag="PLH31_16740"
     CDS             complement(1148..>1500)
                     /locus_tag="PLH31_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR90876.1"
                     /translation="LSISAGIGFIALSGVAVLNGVVMVSFIRSLLAEGRSMDEAISEG
                     ALSRLRPVLMTALVASLGFVPMAFNIVAGAEVQRPLATVVIGGIVSSTLLTLLILPAL
                     YRLAIRSGPGRGQP"
BASE COUNT          285 a          481 c          486 g          248 t
ORIGIN      
        1 ggtgatcgcg tgggcttggc cgacgctctt tcacatctgc gcgccggcga caccctggtg
       61 gtctggaagc ttgatcatct cgggcgctcg ttgaggcagc tcggcgactt cacctctcag
      121 cttgaggcta gacggaccca cttccgccag cctgacggat ggcattgata cggggacagc
      181 ggccggacgt tttttcttcc acgtgatggc cgcgatggcg gaaatggaac gagatctggt
      241 gcgggagcgc acggtcgctg gtttggcggc cgcaaaggcg tgcggaagac tcggtggtcg
      301 cccttcaaag ctgacagcgc agcagatagg acatgcgaaa cgcctgctcg cggatccaga
      361 gatcagcggc gcggaagtcg cccgcacgct cggcgtcgct cgttccacgc tctatcgatc
      421 cattgagcgg cccgccggcg tggcccgtaa acgctaccgt cgtccgtcgt cgagtttgga
      481 gtcaggaacc gcgcgatagg agtcggtcgc gcggtttgga gtcaggattt cgagctaagc
      541 ggtgatgttg agtttcaggg ttaggagtca gtgacaacag gctccaaatc ctgcgtccgg
      601 tctgatcaca attcctgcga ctggcgtggc ggccacggtc gctgcctggc tgtcagaatt
      661 cagaacacgt aaccgccaca gccgccgcat acgtccatcg ccgcctgaga tccgtcggcc
      721 tgcctcgcgt cagcgtcaga gcccgctgcc tcaagcatgg tgtcgcgcgg ctaggcgccg
      781 acatgcttat cgccaaatag cgtcaggtct ctggcgatcc ttacgggaga ctgtccatgc
      841 gacagcagcg gaacaccatg ctggacgctg aaaacggcca ggggatgggt acaggcgcgg
      901 cggatgtgcg ccgccctgcc ctgatcaacg tcaccttgga gacagcgcac gcgcaccgcc
      961 caacaggccg gcggccgagg ccccgatcag gttggatgag cgatcagacc tgctagcggc
     1021 gctcgcgtca ggatgtcgtg acctacccgt gtcagggccg atgccagagg cagaagcgcg
     1081 gcactctggg atcgcctcag aaatcggaca tcgagcgcct caccgatcgg ccgcggcagg
     1141 gataccatca cggctgtccc ctccccggtc cggatctgat cgcaagccga tagagcgcgg
     1201 gcaggatcag cagggtcagc agcgtcgagg agacaatccc gccgatcacc accgtcgcca
     1261 acggacgctg cacctcggcg ccagccacga tgttgaaggc catgggcaca aagcccaggc
     1321 tggccaccag cgccgtcatc agcaccggcc gcagccggct cagggcgcct tcggagatcg
     1381 cctcgtccat cgaccggccc tcggcgagca ggctgcggat aaagctgacc atgacgacgc
     1441 cgttgagcac cgctacgccc gacaaagcaa tgaagccgat gcccgcggag atcgatagcg
//
LOCUS       DAOMEO010000904         1499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8578_length_1499_cov_2.930055, whole genome shotgun sequence.
ACCESSION   DAOMEO010000904 DAOMEO010000000
VERSION     DAOMEO010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_1499_cov_2.930055"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1231)
                     /locus_tag="PLH31_16745"
     CDS             complement(2..1231)
                     /locus_tag="PLH31_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR90877.1"
                     /translation="MSAAARPSLIRSLHASAVKEVVEAHGRFLKGLPGGRRANFSYVN
                     LNNYNFEGLDLSEADLTGALLGGAMLSQARLTRATLYGADLRDADLRECDLTRADMRG
                     ACLRGANLSGADLSACDLREGVTAVQDDTKGLRILKHSTRAGELDYALLNGAKLAGAQ
                     MSGAFAQAADFTDADMTGVTLQGAKLANARMDGADLSGANLHNADMAKVSLRRAVLVG
                     TDLSGANLEDADMAQVLRAPPPIIYVDDLPLNEVLEAHEAFVKSEGREGAVAKAEGVD
                     FRPLRRLKNRRLTGLSAPGAVFFGMDLEGVQLQGANLMGADLRGVTFRNADLRGARLV
                     GAQLAKADLSGAKLGPLKIAEDRFIRTDLSRAVLRGANLTGASAPRARFIEADLTGAR
                     LEGCDLKGAELPATSSS"
BASE COUNT          234 a          531 c          493 g          241 t
ORIGIN      
        1 ctcaagaaga agatgtagcc ggaagctccg cgcccttcag gtcgcaaccc tcgaggcggg
       61 ctccggttag gtccgcctcg atgaaccggg cgcgcggcgc gctggccccg gtcaggttgg
      121 cgccgcgcag caccgcgcgg ctcagatcgg tgcggatgaa ccggtcctcg gcgatcttca
      181 gcggacccag cttggcgccg ctcaggtcgg ccttcgccag ctgggcgccg accaggcgcg
      241 cgccgcgcag atcggcgttc ctgaacgtca cgccgcgcag gtccgcgccc atcagattgg
      301 cgccctgcag ctggacgccc tccaggtcca tgccgaagaa caccgccccc ggcgccgaca
      361 ggcccgtcag ccgccggttc ttcagacgcc gcagcggacg gaagtccacc ccctcggcct
      421 tggcgacagc gccctcgcgc ccctcgctct tgacgaaggc ctcgtgggcc tcaagcactt
      481 cgttcagcgg caggtcgtca acatagatga tcggcggcgg ggcgcgcagc acctgcgcca
      541 tgtcggcgtc ttccagattg gcgccggaga gatcggttcc caccagcaca gcccggcgca
      601 gcgagacctt ggccatgtcg gcattgtgca gattggcgcc cgacaggtca gcgccgtcca
      661 tccgggcatt ggcgagcttc gcaccctgca gggtcacgcc ggtcatgtcc gcgtcggtga
      721 agtccgcggc ctgggcgaag gcgcccgaca tctgcgcccc ggccagcttg gccccgttta
      781 gcagggcgta gtccagctct ccggcccgcg tggaatgctt gaggatccgc aggcccttgg
      841 tgtcgtcctg cacggcggtg acgccctcgc gcaggtcgca ggccgacaga tcagcgcccg
      901 acagattggc tccgcgcagg caggcgcctc gcatgtcggc ccgggtcagg tcgcactcgc
      961 gcagatcagc gtcgcgcagg tcggcgccgt acagcgtggc ccgcgtcagc ctggcctgcg
     1021 acagcatcgc cccgcccagc agcgcgccgg tcaggtcggc ctccgagaga tccagtccct
     1081 cgaagttgta attgttgagg ttcacatagg agaaattggc gcgccgcccg cccggcaggc
     1141 ccttcaggaa ccggccatga gcttccacca cctccttgac ggcggaggcg tgcagggagc
     1201 ggatcagact gggtctggcg gcggcggaca tgcgggatgg gcgtctttca cgagcgatcg
     1261 agcctctcac gtcagcttta aggaaaggtt caaccctgcc tgcaaagcgc ccaccgcccg
     1321 cccgccgcct gtcggacggc aagcttggcc gcaatcgggg gttaaccttg atcatctaga
     1381 cctcagtcgt cattgttgac gcgttcaagg atcgttcacg catggccgag gcgtcattcg
     1441 ttaccccagg atccgccgtc tcggatctga cgggcgaaga gctcatcggg gacctctcg
//
LOCUS       DAOMEO010000905         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8585_length_1498_cov_2.673597, whole genome shotgun sequence.
ACCESSION   DAOMEO010000905 DAOMEO010000000
VERSION     DAOMEO010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8585_length_1498_cov_2.673597"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1498
                     /locus_tag="PLH31_16750"
     CDS             <1..>1498
                     /locus_tag="PLH31_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HQR90878.1"
                     /translation="EFVEAIEAGGVAAMEVLARDLKALGLYTARSLSYDGVEYEIVEH
                     ALSAEQARIYDAYAGAFEIIHNNLSAALDAANITGSGPEGGSRTLNAQAKSAARSAFE
                     SAKQRFFNHLITAMKTPTLISAIDRDLAAGHACVVQIVSTGEALLERRLADIPTEDWG
                     DVTVDITPREYVLDYLAHSFPTQLFEPFTDGEGNLSSRPAFRDGQPVQCRDAVERRDR
                     LIEHLAALAPVQGALDQIIQRFGTEQVAEVTGRSRRVVARTGADGERRLAVEGRAAGA
                     NLAETAAFQDDDKRILIFSDAGGTGRSYHADLGAKNQRLRIHYLLEAGWKADTAIQGL
                     GRTNRTHQAQPPVFRPISTNVKAEKRFLSTIARRLDSLGAITKGQRQTGGQGLFRAED
                     NLESPYAKAALRQLYMLLLLGKVPGCSLQRFEDATGLKLTDRDGTIREDLPPITQFLN
                     RMLALTIALQDTLFEVFEGLLRAKIEGAISAGVYDRGVETITAHSLRVT"
BASE COUNT          262 a          529 c          474 g          233 t
ORIGIN      
        1 cgagttcgtc gaggcgatcg aggccggcgg cgtggcggcg atggaggtgc tcgcgcgcga
       61 tctgaaggcg ctcggcctct ataccgcgcg atccctctcc tatgacggcg tcgaatacga
      121 gatcgtcgaa cacgccctca gcgctgaaca ggcgaggatc tacgacgcct atgccggcgc
      181 cttcgagatc atccacaaca acctgtcggc cgcgctggac gccgccaaca tcaccggctc
      241 tggccccgaa ggcggatcgc gaacgctgaa cgcccaggcc aaatcggccg cccgctcggc
      301 ctttgaaagc gccaaacagc gcttcttcaa tcacctgatc accgcgatga agacgccgac
      361 cctgatcagc gccatcgacc gcgatctcgc cgccgggcac gcctgcgtcg tccagatcgt
      421 ctccaccggc gaagcgctgc tggagcgccg cctcgccgac atcccgaccg aagactgggg
      481 cgacgtgacg gtcgacatca cgccgcgcga atatgtcctg gactatctcg cccattcctt
      541 ccccacccag ctcttcgagc cgttcaccga tggcgagggc aacctctcgt cacggccggc
      601 ctttcgcgac ggccagcccg tgcaatgccg cgatgcggtc gagcgccgcg accgactgat
      661 cgagcatctg gccgcgctgg cgccggtcca gggcgcgctc gatcagatta tccagaggtt
      721 cgggaccgaa caggtcgccg aggtgacggg ccggtcgcgc cgcgtggtcg cccgcacggg
      781 cgcagatggc gagcggcgcc tcgccgtgga agggcgcgct gcgggcgcca atctcgccga
      841 gaccgccgcc ttccaggatg acgacaagcg cattctgatc ttctccgatg cgggcggcac
      901 cggccgcagc tatcacgccg atctgggcgc gaagaaccag cgcctgcgca tccattatct
      961 gctggaggcg ggctggaagg cggacaccgc catccagggt ctggggcgca ccaaccgcac
     1021 gcatcaggcc cagccgccgg tctttcggcc gatttcgacc aatgtgaagg ccgagaagcg
     1081 tttcttgtcg accatcgccc ggcggctcga cagcctgggc gcgatcacca agggtcagcg
     1141 gcagaccggc ggccaagggc tgttccgagc cgaggacaat ctcgaaagcc cctacgccaa
     1201 ggctgcgctg cggcagctct atatgctgct cctgctcggc aaggtgccgg gctgctcgct
     1261 ccagcggttc gaggacgcca cgggtcttaa gctcaccgat cgcgacggca cgatccgcga
     1321 ggatctgccg cccatcaccc agtttctcaa tcggatgctg gcgctgacga tcgcgcttca
     1381 agacaccttg ttcgaagtgt tcgagggcct gctgcgggcc aagatcgaag gggcgatcag
     1441 cgccggcgtc tatgaccgcg gcgttgaaac catcaccgcg cactcgctgc gggtcacc
//
LOCUS       DAOMEO010000906         1498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8589_length_1498_cov_1.760915, whole genome shotgun sequence.
ACCESSION   DAOMEO010000906 DAOMEO010000000
VERSION     DAOMEO010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_1498_cov_1.760915"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="PLH31_16755"
     CDS             <1..79
                     /locus_tag="PLH31_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQR90879.1"
                     /translation="TGGPSSGAAPRDAQPEGAGDLRPAQ"
     gene            87..896
                     /locus_tag="PLH31_16760"
     CDS             87..896
                     /locus_tag="PLH31_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR90880.1"
                     /translation="MARTRADTGFSPALIGSVALHGLVAAAILIGLPWKKPTPITLGE
                     SVPVTIVTNGPTNVRPSEEALEEQTALTEEPVAEATPTPPAPVPAPVPTPAPTPPSKA
                     APKPTPTPTPAKKPTPAKANDDFFASLEASLSKTKKSTGKPAANAPRGPTRAETSVSA
                     RPAAGAATGLSAAALGRLQGEVQDRWNPNCEVEGGSNVNVRVVFVIGPGGRVVGQPES
                     PGTSSPDPVVKAASDRAVRALLQASPFAYLPSDLYGQKIALNFNAKQACSR"
     gene            917..>1498
                     /gene="tolB"
                     /locus_tag="PLH31_16765"
     CDS             917..>1498
                     /gene="tolB"
                     /locus_tag="PLH31_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921063.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HQR90881.1"
                     /translation="MRLKALLLMVAALIGGAMVSPAAAQIEVDINAGAVKPLPIAVPA
                     FSGTPRGAEIAQVITGNLERSGLFQPLNVSAVADKLPDVNVQPRFPDWQATGAQALIN
                     GQVTVGADGGLRVDFRLWDVFSQQQLLGLQFSSTPDNWRRVAHKISDAVYERLTGEKG
                     YFDTRVAFVAESGPKMARVKRLAIMDQDGANPQF"
BASE COUNT          242 a          552 c          484 g          220 t
ORIGIN      
        1 aacgggtggc ccctcctcgg gggccgcgcc gcgcgacgcc cagcccgagg gcgcgggcga
       61 cctgcggccg gcccaatagg tcgctgatgg cccgtactcg cgccgacacc ggattctcgc
      121 ccgccctgat cgggtcggtc gcgctgcacg ggctggtagc ggcggcgatc ctcatcggtc
      181 tgccgtggaa gaagcccacg cccatcaccc tgggcgaaag cgtgccggtg accatcgtca
      241 ccaatggccc gaccaatgtg cggccgtccg aggaagcgct cgaagagcag acagccctga
      301 ccgaggagcc ggtagccgag gctaccccca ctccgccggc tcccgtgcct gcgcccgtgc
      361 cgacgcccgc cccgaccccg ccgtcgaagg ccgcgcccaa gccgaccccc accccgacgc
      421 ccgccaagaa gccgaccccg gccaaggcca atgacgactt cttcgcctcg ctggaggcgt
      481 cgctgtccaa gaccaagaag tcgaccggca agcccgccgc caatgcgccc agaggtccaa
      541 cccgcgcgga gacttccgtg tcagcacgcc ccgccgccgg cgccgctacg ggattgtccg
      601 ccgctgcgct tggccgtttg cagggcgagg tgcaggatcg ctggaacccc aattgcgagg
      661 tcgagggcgg ctcgaacgtc aatgtgcgcg tggtgttcgt gatcggcccc ggcggccgcg
      721 tggtgggcca accggaatcg ccgggcacga gttcgccaga cccggtggtc aaagcggcgt
      781 ccgaccgcgc cgttcgcgcg ttgttgcagg cttcgccgtt cgcctatctg ccgtccgacc
      841 tttacggtca gaaaatcgcc ctgaatttca acgcaaaaca ggcctgctcg cgatgatgtc
      901 gaacaaggaa aacccgatgc gcttgaaagc cttgctgctg atggtcgccg cgctgatcgg
      961 cggagccatg gtttcccccg ccgccgccca gatcgaggtc gacatcaatg ccggcgcggt
     1021 caagccgctg ccgatcgccg tgccggcctt ttccggcacg ccgcgcggcg ccgaaatcgc
     1081 ccaggtcatc accggcaatc ttgagcgctc gggcctgttc cagccgctga acgtctcggc
     1141 cgtggccgac aagctgcccg acgtgaacgt ccagccgcgc ttccccgact ggcaggccac
     1201 cggggcccag gccctgatca atggccaggt gacggtaggc gccgacggcg gcctgcgcgt
     1261 cgacttccgc ctgtgggacg tgttttccca gcagcagctg ctgggcctgc agttttcctc
     1321 gacgcccgac aactggcgcc gggtggccca caagatcagc gacgccgtct atgagcgcct
     1381 gaccggcgaa aaaggctatt tcgacacccg cgtcgccttc gtggccgaga gcggtccgaa
     1441 gatggcccgg gtcaagcgcc tggcgatcat ggaccaggac ggcgccaatc cgcagttc
//
LOCUS       DAOMEO010000907         1497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8600_length_1497_cov_1.883495, whole genome shotgun sequence.
ACCESSION   DAOMEO010000907 DAOMEO010000000
VERSION     DAOMEO010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_1497_cov_1.883495"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1497)
                     /locus_tag="PLH31_16770"
     CDS             complement(119..>1497)
                     /locus_tag="PLH31_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR90882.1"
                     /translation="NFLPISSDKYLKLGGGLERTQEAFRQYSRRDAEVLPAYYAMLDE
                     IGDVLRDLAAETPPNLGDGIHGLLRGARQGTRLALLRRERKRDLLDLLTKSARDFLDG
                     WFESDAVKASFGFDAVVGNFASPDSPGSAYVLLHHTFGEVNGKKGAWGHAIGGMGAIT
                     QAMAKACEALGVEILLDAPVEAVHVEHGKDGPRATGVQLVDGRQIMAPIVSSNLNPAL
                     LYGKLIAPSALPAAFTKAIKGYKNGSGTFRMNVALSELPDFTCLPGKVSAEHHQCGIV
                     ISPSLDYMDEAYRDAKAFGISKKPIVEMLIPSTLDPTLAPPGQHVASLFCQQFAWDLP
                     NGRSWDDEREAAADLIIDTVNDWAPNFKASVLGRMILSPTDLERKFGLINGDIMHGHM
                     SLDQLWAARPVLGHASHRGPLKGLYMCGAGCHPGGGVSGNPGRNAAREILRDKDFTTA
                     LKLSVVGR"
BASE COUNT          229 a          499 c          525 g          244 t
ORIGIN      
        1 aggggtgggg gtgacacggt ttcaggatgt ggcgcggccg agctgaaagc tgccgacgct
       61 gtcaccccca tccccgaccc ttcccccatc aagggggaag ggagatgcgt gaccaccatc
      121 accgccccac caccgaaagc ttcagcgccg tcgtgaagtc cttgtcgcgc aggatctcgc
      181 gcgccgcgtt gcgtcccgga ttgcccgaga cgccgccgcc cggatggcaa ccggcgccgc
      241 acatgtagag gcccttcagg gggccgcgat ggctggcgtg ccccagcacc ggtcgcgcgg
      301 cccacagctg atccagcgac atatggccgt gcatgatgtc gccgttgatc aggccaaact
      361 tccgctccag gtccgtcggc gacaggatca tccgtcccag gaccgatgcc ttgaagttgg
      421 gcgcccagtc attgaccgtg tcgatgatca ggtcggcggc ggcctcgcgc tcgtcatccc
      481 acgaccggcc gttcggcagg tcccaggcga actgctggca gaacaggctg gccacgtgct
      541 ggccgggcgg ggccagggtg gggtccaggg tcgaggggat cagcatctcg acgatgggct
      601 tcttgctgat gccgaaggcc ttggcgtctc tataggcctc gtccatgtag tcgaggctgg
      661 gcgagatcac gatgccgcac tggtgatgct cggccgagac cttgccgggc aggcaggtga
      721 agtccggcag ctccgacagg gccacgttca tccggaaggt gcccgagccg ttcttgtagc
      781 ccttgatggc cttggtgaag gcggcgggca gggccgaggg ggcgatcagc ttgccgtaca
      841 gcagcgccgg attgaggttg gacgagacga tcggggccat gatctgacgg ccgtcgacca
      901 gctgcacgcc cgtggcccgg gggccgtcct tgccgtgttc gacatgcacc gcctcgaccg
      961 gcgcatcgag caggatctcg acgcccagcg cctcgcaggc cttggccatc gcctgggtga
     1021 tcgcgcccat gccgccgatg gcgtgccccc aggcgccctt cttgccgttc acctcgccga
     1081 aggtgtggtg cagcagcaca taggccgagc cgggcgaatc cggactggcg aaattgccga
     1141 ccacggcgtc gaagccgaag ctggccttga ccgcgtcgct ctcgaaccag ccgtccagga
     1201 agtcgcgggc gctcttggtg agcaggtcca gcaggtcgcg cttgcgctcg cgcctcagca
     1261 gggccagccg cgtgccctgg cgggcgccgc gcagcaggcc gtggatgccg tcgcccagat
     1321 tgggcggggt ctcggcggcc aggtcgcgca gcacgtcgcc gatctcgtcc agcatggcgt
     1381 agtaggccgg cagcacctcg gcgtcgcggc ggctgtactg gcggaacgcc tcctgggtgc
     1441 gctccagacc gccgcccagc ttgaggtact tatctgagct gatcggcagg aagttgg
//
LOCUS       DAOMEO010000908         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8604_length_1496_cov_2.739070, whole genome shotgun sequence.
ACCESSION   DAOMEO010000908 DAOMEO010000000
VERSION     DAOMEO010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8604_length_1496_cov_2.739070"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLH31_16775"
     CDS             complement(<1..931)
                     /locus_tag="PLH31_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR90883.1"
                     /translation="MRDGPWAAEQRIERLNRCIDLLIGHQADIAKAVSTDFGSRSPEA
                     TSLTDVAGSIGPLKFARDNLAKWMKPQKRKTTPAILGLFGAKASVQYQPKGVVGVISP
                     WNFPVNLTFAPLAGILAAGNRAMIKPSEFTPATSDLMKAMFAKAFTDEEIAVFVGGAE
                     AGQAFSGLAFDHLVFTGATSVARHVMRAAAENLVPVTLELGGKSPVILSRGADMATAA
                     ARIMNGKTLNAGQICLAPDYVFAPADQVDTFVAEAKAAVGRYFPTLKDNPDYTAVVAQ
                     RHFDRITGYVEDARARGATIIEINPAGEDLSQQP"
     gene            857..1069
                     /locus_tag="PLH31_16780"
     CDS             857..1069
                     /locus_tag="PLH31_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90884.1"
                     /translation="MAYQQIDATVEPLDALLGCPGPIAQICRFLAFQGMIHLGFVSFE
                     RQIRRCVHGRDSSRRPATGSSYHWLQ"
     gene            1199..>1496
                     /locus_tag="PLH31_16785"
     CDS             1199..>1496
                     /locus_tag="PLH31_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR90885.1"
                     /translation="MSDVEITLRGPEAYKIAKRALELMERHQVWPTALNFELWTHYVA
                     DPDGALARELTRLISLGEPMTEMVSEELAATYLPKARLNEQIRDAGDLLSKELEA"
BASE COUNT          269 a          474 c          477 g          276 t
ORIGIN      
        1 ggggctgctg tgacagatcc tcgcccgccg gattgatctc gatgatcgtc gcgcccctgg
       61 cgcgggcgtc ctcgacatag ccggtgatcc ggtcaaagtg gcgctgggcg accacggcgg
      121 tgtagtcggg attgtccttc agagtcggga aatagcgacc gacggcggcc ttggcctcgg
      181 ccacgaaggt atcgacctgg tcggccggcg cgaagacata gtcgggcgcc aggcagatct
      241 ggccagcgtt cagggtcttg ccattcatga tccgggccgc ggccgtggcc atgtcggcgc
      301 cgcgcgacag gatcaccggg ctcttgccgc ccagttccag ggtcaccggc accaggttct
      361 ccgccgccgc gcgcatgaca tggcgcgcca ccgaagtcgc gccggtaaag accagatggt
      421 caaaggccag cccggagaag gcttgtccgg cctcggcgcc gccgacgaag accgcgatct
      481 cttcgtccgt gaaggccttg gcgaacatcg ccttcatcag gtccgaggtc gccggcgtga
      541 attccgacgg cttgatcatc gcccgattgc cggcggccag gatgccggcc agcggcgcga
      601 aggtcaggtt gaccgggaag ttccaggggc tgatcacccc gaccacgccc ttgggctggt
      661 actgaacgct ggccttggcg ccgaacaggc ccaggatcgc aggggtggtc ttgcgcttct
      721 gcggcttcat ccatttggca agattgtcgc gcgcgaactt caacggcccg atcgagcccg
      781 ccacgtcggt cagggacgtg gcttccggtg aacgagaccc aaaatcggta ctgactgctt
      841 tggcaatatc cgcctggtgg cctatcagca gatcgatgca acggttgagc cgctcgatgc
      901 gctgctcggc tgcccagggc ccatcgcgca aatatgccgc tttttggcgt tccagggcat
      961 gattcatctc ggctttgtga gcttcgagcg tcagattcgt cggtgcgttc atggtcgcga
     1021 ttcctcccgg cggcccgcta cgggctcatc ttatcattgg ttacaatgat cgccggaatc
     1081 gccaagcgca agagaacctt gttcgctctt aacgcctcgt ttgaactctc cgagctagag
     1141 tgaagatcca agcgatcggc attctcgctg cgggaacgcg ccgacggggg aagaaaagat
     1201 gtcggacgtc gaaatcacac tgcgcggccc cgaggcctac aagatcgcca agcgcgctct
     1261 ggaactgatg gagcgtcatc aggtttggcc caccgcgctg aatttcgaac tctggaccca
     1321 ctacgtcgcc gatccggacg gcgctctggc ccgcgaactc actcggctga tttcgcttgg
     1381 cgagcccatg accgagatgg tcagcgagga actggcggcc acctatctgc ccaaggcgcg
     1441 cctcaacgaa cagatccgcg atgcgggcga cctgctcagc aaggaactgg aagccg
//
LOCUS       DAOMEO010000909         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8605_length_1496_cov_2.669674, whole genome shotgun sequence.
ACCESSION   DAOMEO010000909 DAOMEO010000000
VERSION     DAOMEO010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8605_length_1496_cov_2.669674"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1415)
                     /locus_tag="PLH31_16790"
     CDS             complement(3..1415)
                     /locus_tag="PLH31_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HQR90886.1"
                     /translation="MRDKLFIGPKVRLLRQAKGWKLEPCAARLGVSASYLSQIEANQR
                     PVTARVLMDVMRVFEVDAASLDAADDQRLIADLREATADPIQDGETPSLSELKSAVAN
                     TPNLAKAYLALHHAYRRLDERLKTTDEAVSLDERGAASALLPYEEVRDFFHYKNNYIH
                     SLDMASEALAAELGLDGAEATETVLERQLRDGLGIRVVRSAEPDLLRRFDPAAGLLTL
                     GAALAAATRSFQMAYQIVAAALSDTVEGELTAAGFRSEGAAKVCRTALLNYAAGALLL
                     PYDRFREAARAARHDTEQLAQTFQTSLEQVFHRLSTLQRPGARGVPFYFVRVDQAGNI
                     TKRHSATRLQFARFGGTCPLWNVHDAFARPDQWLVQLAEMPDGVRYVSIARGLVKPSG
                     DYLRPGRRYALGLGCEVEYADSLVYAQGLDLAGPSAPIGVSCRICERDDCSQRAFPPV
                     DRPFEVLENERRWVPFFLDR"
BASE COUNT          239 a          503 c          494 g          260 t
ORIGIN      
        1 acctagcggt ccagaaagaa gggcacccag cgccgttcgt tctcaagcac ctcgaacggt
       61 cgatcgacgg ggggaaaggc ccgctgggag caatcatcgc gctcgcagat tcggcaactg
      121 accccgatcg gggctgacgg cccggccaag tccagtccct gagcgtagac caggctgtcg
      181 gcatattcga cctcgcagcc cagccccagg gcgtagcgac ggccaggccg cagatagtcg
      241 cctgacggct tcaccagtcc gcgcgcgatg ctgacatagc gcactccgtc gggcatctcg
      301 gccaactgga ccagccattg atcaggccgc gcgaaggcgt cgtggacatt ccacagcgga
      361 caggtcccgc cgaaccgcgc gaactgcagc cgggtggcgc tgtggcgctt ggtgatgttg
      421 cccgcctggt cgacccgcac gaaatagaat ggcacgcccc gcgcgccggg ccgctgcagg
      481 gtgctcagcc gatgaaacac ttgctccagg ctggtctgga atgtctgcgc cagttgctcg
      541 gtgtcatgcc gcgccgcgcg cgccgcctcg cggaagcggt catagggcaa gagcagcgcg
      601 ccggccgcgt agttcagcag ggctgtacga cagaccttgg ccgccccttc gctgcggaac
      661 cccgccgccg tcaactcgcc ctcgacggtg tcggatagcg cggcggccac gatctggtag
      721 gccatctgga agcttcgtgt ggccgcggcc agggccgcgc ccagggtcag caggccggcg
      781 gccggatcga accggcgcag caggtccggc tcggccgatc gcaccactcg gatacccagt
      841 ccgtccctga gctggcgctc cagcaccgtc tcggtcgctt cggctccgtc caagccgagc
      901 tcggcggcca gggcttcgga cgccatgtcc aggctgtgga tgtagttgtt cttgtagtga
      961 aagaagtcgc gtacctcttc gtagggcaga agtgcgctgg ccgcgccgcg ttcgtccagc
     1021 gacacagcct catcggtggt ctttagccgc tcgtccagcc ggcgatacgc gtggtggagg
     1081 gccagatagg ccttggcgag gttgggcgtg ttggccaccg ccgacttcag ttcggagaga
     1141 ctgggggtct cgccgtcctg gattggatca gcggtcgctt cgcgcaggtc ggcgatcagc
     1201 ctctggtcgt cggcggcgtc caacgaggcg gcgtcgactt cgaacacccg catcacgtcc
     1261 atcagcaccc ttgccgtcac cggccgctgg ttggcctcga tctgcgacag atagctggcc
     1321 gagacgccca ggcgcgcggc gcacggctcc agcttccaac ccttggcctg acgcaggagg
     1381 cggaccttcg ggccgatgaa cagcttgtcg cgcacggctt tacaactttg cagttttaca
     1441 gatttagccg tcgcaacccc gcttggcggc tcgattttgc gcatgttttt cttgcg
//
LOCUS       DAOMEO010000910         1496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8606_length_1496_cov_2.612769, whole genome shotgun sequence.
ACCESSION   DAOMEO010000910 DAOMEO010000000
VERSION     DAOMEO010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_1496_cov_2.612769"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLH31_16795"
     CDS             <1..667
                     /locus_tag="PLH31_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR90887.1"
                     /translation="STVLVFGGLAAARLGHVATHWEYFGADPWRSLAVWQGGFSWIWV
                     APVVILTTILLLRTTRERAWAIAPVLASALVWTTAHQLASATEPMAPPALTLAAMDAP
                     ALDLSAPTDRPTVINLWATWCPPCVREMPALAQAEQAHPNVRFLFVNQGEGEAGVRAF
                     LKSQGLALDHVLFDEAMAVPRHYGTAGIPVTLFLHADGRLAKAHMGEIAPEQIATEIA
                     RLH"
     gene            690..1364
                     /locus_tag="PLH31_16800"
     CDS             690..1364
                     /locus_tag="PLH31_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR90888.1"
                     /translation="MNRTFAPAFAAVLALAACGPADADNSAAPANPSAASGQDMPAIA
                     QVGFNDLLRDPAAPFIGAENADVTIIGFVDYNCPYCKKMQPEIDGLLKADPKVRVLYK
                     DWPIFGDVSETAARTALAAGYQGKYEAVHNAFMLSPSPIADQADITRLVQSADVDMAR
                     LNQDLADHGADIDAVLERNNREAAALALRGTPAFIINGNLIPGGMPQAQLEALIGRIR
                     AGQPLR"
BASE COUNT          227 a          559 c          469 g          241 t
ORIGIN      
        1 gagcacggtc ctggtcttcg gcggtctggc ggccgcccgc ctcggtcacg tggcgacgca
       61 ctgggagtat ttcggggccg acccgtggcg atccctggcg gtctggcagg gcgggttcag
      121 ctggatctgg gtcgcgccgg tcgtgatcct gaccacgatc ctgctgctgc gcactacgcg
      181 cgaacgcgcc tgggccattg cgcctgtgct ggccagcgcc ctggtttgga ccaccgccca
      241 tcagttggcc tcggcgacgg agcccatggc tccgcccgcc ctgacgttgg ccgcgatgga
      301 cgcaccggcg ctcgaccttt cggcgcccac cgaccggccc acggtgatca atctctgggc
      361 cacctggtgc ccgccgtgcg tgcgcgagat gccggctctg gctcaagctg aacaggctca
      421 tccgaacgtg cgcttcctct tcgtcaacca gggcgagggc gaggccggcg ttcgcgcctt
      481 cctgaagagc caggggctgg ccctcgacca tgtcctgttt gacgaggcca tggcggtgcc
      541 gcgccactac ggcacggccg gcattccggt gaccctgttc ctccacgccg acggccgcct
      601 ggccaaggcc cacatggggg aaatcgcccc cgaacagatc gcgaccgaaa tcgcgcgtct
      661 gcactgaccc ctcgcctcac ggagcttcca tgaaccgcac cttcgccccc gctttcgccg
      721 ccgtccttgc gctcgccgcc tgcggccccg ccgacgccga caacagcgca gcgcccgcca
      781 atccctccgc cgcgtccggg caggacatgc ccgccattgc gcaggtcggc ttcaacgacc
      841 tgcttcgcga tcccgccgcg cccttcatcg gcgccgaaaa cgccgatgtg accatcatcg
      901 gcttcgtcga ttacaattgc ccctactgca agaagatgca gccggagatc gacggcctgc
      961 tgaaggcgga ccccaaggtg cgcgtcctgt acaaggactg gccgattttc ggcgacgtct
     1021 cggagaccgc cgcccggacc gccctggccg ccggctatca gggcaaatac gaggccgtcc
     1081 acaacgcctt catgctgtcg ccctcgccta tcgccgacca ggcggacatc acgcgtctgg
     1141 tccagtcggc cgacgtggac atggcgcgtc tgaatcagga cctggccgat cacggcgcag
     1201 acattgacgc ggtgctggag cgcaacaacc gcgaggcggc ggccctggcc ctgcggggca
     1261 cgcccgcctt catcatcaac ggcaatctga ttcccggcgg catgccccag gcgcaactgg
     1321 aagcgttgat cggccgtatc cgcgccggac aacctctgcg ctgaggccga ccatgaccgc
     1381 cctgaccctg ctccaactga acgacctgca cggctatctc gagccgcatc cggaattggt
     1441 gcgcaccgaa ggcggctggc ggtttgaacg gctgggcggg gtcgcccgca tcgccc
//
LOCUS       DAOMEO010000911         1495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8615_length_1495_cov_1.645139, whole genome shotgun sequence.
ACCESSION   DAOMEO010000911 DAOMEO010000000
VERSION     DAOMEO010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8615_length_1495_cov_1.645139"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1495)
                     /locus_tag="PLH31_16805"
     CDS             complement(<1..>1495)
                     /locus_tag="PLH31_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90889.1"
                     /translation="QRRAIEHITGPERIAAVVGYAGAGKSTMLAAAREAWEAEGYTVH
                     GAALSGKAAEGLEESSGIQSRTLASWSRGWDNPHSGDRYALGRGDVFVIDEAGMVGSR
                     QLARFVGEAEARGAKIVLVGDHEQLQAIGAGAPFRAITEEIGHAELSEIRRQRVDWQR
                     EASVDFATHRTAEGLAAYRDRGDIRFSETGEDARGEIVRDYLADRDQRPDGTRVAMAH
                     RRADVRAINDAIRTELQDRGELAQGNVPAVGSDAGALTFQTNDGSREFAPGDRIVFLE
                     NNRDLGVKNGMLGTVEHVEPGRIIATLDGRGGESVSMPMGDYQAIDHGYATTIHKNQG
                     ATVDRSYVLASGTMDRHLTYVAMTRHRDGVQLYAAQDEFTNAGRLVEHGAAPYEHDPQ
                     KSDSYFVTLENDKGEQRTLWGVDLERAMKEAAPEIGEKIGLQHEGSTPVTFPDGTQTH
                     RNTWKVQDAGELAYSQLERRLSRSGVKETTLDYTSDFAERRGIAEQMG"
BASE COUNT          218 a          543 c          454 g          280 t
ORIGIN      
        1 cgcccatttg ctccgcgatc ccgcgccgct cggcaaagtc gcttgtataa tcgagcgtgg
       61 tttccttcac gcccgaccgg gacaggcggc gttctagctg gctataggcc agctcgccag
      121 cgtcctgaac cttccaggtg ttgcggtgcg tctgcgtgcc atccggaaag gtgacgggcg
      181 tggaaccttc atgttgaagg ccgatcttct ccccaatctc cggcgcggcc tccttcatgg
      241 cccgctcaag atcgacgccc catagggtgc gctgctcgcc cttgtcgttt tccagcgtca
      301 cgaaatagct gtcggacttc tgcgggtcgt gctcataggg cgcggcccca tgctcgacca
      361 ggcggcccgc attggtgaac tcgtcctgtg ccgcgtagag ctgaacgccg tcccgatgcc
      421 gggtcatggc gacataggtt agatgccggt ccatcgtccc cgacgccagc acataggacc
      481 gatcgaccgt cgccccctga tttttgtgaa tcgtcgtcgc atagccgtgg tcgatcgcct
      541 gataatcgcc catcggcatg ctgacgctct cgccgccccg gccgtctagg gtggcgatga
      601 tgcggcccgg ctcgacatgc tcgaccgttc caagcatccc gtttttcacg ccaaggtcgc
      661 ggttgttctc caagaacacg atgcggtcgc ccggcgcgaa ctctcggcta ccgtcattgg
      721 tctggaaggt cagcgcgccg gcgtccgagc ccacggcggg cacgttgccc tgcgccagct
      781 cgccccggtc ctgtagctcg gtcctgatcg cgtcattgat cgcccgaaca tcggcccggc
      841 gatgcgccat cgcaacccgg gtgccgtccg ggcgctgatc tcgatcggca agataatcgc
      901 gcacgatctc gccgcgcgcg tcctcgccgg tctcactgaa acgaatgtcg ccccggtccc
      961 gataggccgc cagcccctcg gccgttcggt gcgtggcgaa gtcaaccgac gcctcccgct
     1021 gccagtccac gcgctgccgg cgtatctcgg aaagctcggc atggccgatt tcctccgtga
     1081 tcgcccggaa cggtgcgccg gccccgatcg cctgtagctg ctcatggtcg ccgactagga
     1141 cgatcttcgc cccgcgcgcc tcggcctcgc caacgaagcg ggcgagctgc cggctcccga
     1201 ccatgccggc ctcgtcgatg acaaacacgt cgccgcggcc gagtgcatag cggtccccac
     1261 tatgtgggtt gtcccaaccg cgggaccatg acgccagtgt gcggctctgg atgccggaag
     1321 attcttccag gccctcggcc gccttgcccg acaaggccgc gccgtggacc gtgtagccct
     1381 cggcctccca tgcctcgcgc gccgccgcga gcatggtgga cttgccagcg ccggcataac
     1441 cgacaacggc cgcgatccgc tccggcccgg tgatatgctc gatcgcccgg cgctg
//
LOCUS       DAOMEO010000912         1494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8620_length_1494_cov_1.651842, whole genome shotgun sequence.
ACCESSION   DAOMEO010000912 DAOMEO010000000
VERSION     DAOMEO010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8620_length_1494_cov_1.651842"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    275..322
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(380..910)
                     /locus_tag="PLH31_16810"
     CDS             complement(380..910)
                     /locus_tag="PLH31_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQR90890.1"
                     /translation="MKNLMPAALAALSVAAIPALSHAQVTPEYYGSAGYGTTRADGAD
                     LGAIQGRLGAKLTPHFGVEGEVGVGVSDDKTYVAGAPVNVELKHQAAAYAVGFLPVTP
                     QIDLLARAGYGTTKFKSSVAGLSASESQDSWNYGVGAQYKLDDRNGLRADWTKSEYTN
                     SKADADTLAVSYVRKF"
     gene            1208..>1494
                     /locus_tag="PLH31_16815"
     CDS             1208..>1494
                     /locus_tag="PLH31_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516882.2"
                     /note="catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQR90891.1"
                     /translation="MHHAVIAATGLYTPPHSISNTELVEAFNTFVERFNTANAEAIAA
                     GAVLALAPSSPEFIEKASGIKSRFVMNKDGIIDPDLMRPVLPERSNDEISIL"
BASE COUNT          241 a          482 c          460 g          263 t
ORIGIN      
        1 cccgccgccg caatggtcgc tggccttgcg ccctttcctc gcgacgaggt ggggacagaa
       61 tacgggtagt ttgagcgcgg aggattggcg cgatcgctct attttcaggg cgttgatttc
      121 acttaacgtc ctgctcgaaa gcacggggat tgatcgagct ctatccccgt actcgccccc
      181 tcctgacctg cttcgcaggt cgtcctcccc cagcggggga agacgaaatc atcctcccct
      241 gctgggggag gacgaccgcg tagcggtcag gaggnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nncgggccgc cgcgggacca gagcccgtcc cgggcctctc
      361 ggccggggac aaatccgccc tagaacttgc ggacgtagga caccgccagg gtgtcggcgt
      421 cagccttgga gttggtatat tccgacttgg tccagtcggc ccggagaccg ttcctgtcgt
      481 ccagcttgta ttgagcgccg acgccatagt tccagctgtc ctgcgactcg ctggccgaca
      541 ggccggcgac cgagctcttg aacttggttg tgccgtaacc ggcccgggcc agcaggtcga
      601 tctgaggcgt cacgggcagg aagcccaccg cataggcggc cgcctggtgc ttgagttcga
      661 cgttcaccgg cgcgccggcc acataggtct tgtcgtcgga aacgccgacg ccgacctcgc
      721 cttcaacgcc gaaatgcggg gtcagcttgg cgccaagccg gccctggatc gcgcccagat
      781 cggcgccgtc ggcgcgggtg gttccgtagc cggccgagcc gtaatattcc ggcgtgacct
      841 gagcgtgcga cagggcggga atggcggcga ccgacagggc ggctagagcc gcgggcatga
      901 gatttttcat tgtttttact ccaggtactg attgcccctc agccccctct gactaggcgc
      961 gccatgcggc cccgatgggg cccggaccgg gcctgtttga ggccattgcg tgtcgcagcc
     1021 gacatgtcca aggcgattgt ggcgacgcgg gattgttccc ctgcggaagc gaaaacagcc
     1081 cgcctgtgac ctcaaggccg cgcgtccggg ggaggtgacg aggccggcag gcgtgctatt
     1141 gatccagcag atcaacaatt gagacgcgcg ctccaggtcg cgcgccagcg tcatcgggac
     1201 cagtcgcttg catcacgccg tcatcgccgc cacggggctc tacaccccgc cccacagcat
     1261 ttccaacacc gagctggtcg aggccttcaa caccttcgtc gagcgcttca acaccgccaa
     1321 tgccgaggcc atcgcggccg gcgcggtatt ggccctggcc ccctcctcgc ccgaattcat
     1381 cgagaaggct tccggcatca agtcgcgctt cgtgatgaac aaggacggca tcattgatcc
     1441 cgacctgatg cgtccggtcc tgcctgaacg gtccaacgac gagatctcga tcct
//
LOCUS       DAOMEO010000913         1491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8632_length_1491_cov_2.360724, whole genome shotgun sequence.
ACCESSION   DAOMEO010000913 DAOMEO010000000
VERSION     DAOMEO010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_1491_cov_2.360724"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLH31_16820"
     CDS             complement(<1..239)
                     /locus_tag="PLH31_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90892.1"
                     /translation="MSLVEAQGIVQVACANGYFGLALQARRIDIELERRGPDVVSVAS
                     NLSRIIFNVVGFRIVGVGVLSVCEPAVETGHRHQVA"
     gene            390..>1491
                     /locus_tag="PLH31_16825"
     CDS             390..>1491
                     /locus_tag="PLH31_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR90893.1"
                     /translation="MAVTKPTNLIFYGPPGTGKTYRTALEAVRLCDGDTPDERSDLVR
                     RYGELEREGRIGFVTFHQSFSYEDFVEGLRPETAASDISGSAGFRLEPRNGVFREMAA
                     LADQARLAAAAPRRAGGLDLSGRRFWKMGLGAIGTEDHIYDEAIAGDYVVLGWGGEVD
                     WSDAAYEDIGAVRAKWAEVGPADSKPSNVSQLHPFRSEMKKGDIIIVPYGNTAFRAIG
                     EVTGDYTFVANPDGEYNHRREVNWLLKLEKPLPLDSIVEGAFTMRTLYPINASRIRME
                     AVSRLIGAPGDAEATAPGVAPSFVLVIDEINRANISKVFGELITLLEADKRLGMENEI
                     RVKLPYSGEMFGVPANLHLIGTMNTADRSIALL"
BASE COUNT          276 a          454 c          503 g          258 t
ORIGIN      
        1 gcaacctggt ggcggtgacc cgtctctacg gcaggttcgc aaacggagag gaccccaacg
       61 cccacgatcc ggaagccgac gacgttgaag atgatgcggc tgaggttgga agcgacgctg
      121 acgacatccg ggccgcggcg ctcaagctct atatcgatcc ggcgcgcctg aagggcgagg
      181 ccgaagtatc cattcgcgca ggcgacttgc acaatgccct gggcctcgac caggctcacg
      241 ccaacgtctg ccaagcgctg ggcggcgcca agtttcagca gttggccaat gtgcagccgc
      301 cgcgagtaga ggggccgggg caaagctcga cgacaaccta ccactttagc cttcgggcag
      361 gctcgagcct gactactgag agtgcggcct tggcggtcac gaagcccacc aacctgattt
      421 tctacggacc accaggcacc ggtaagacct accggaccgc gctggaggct gtgcggctgt
      481 gcgatggcga tacgcccgat gagcgtagtg atctggtgcg acgctacggc gagcttgagc
      541 gggaggggcg gatcggcttc gtcacgttcc accagagctt ctcttacgag gactttgttg
      601 aggggctgcg tccggagacg gcggcgtccg acatcagcgg ctcagctggt ttccggctgg
      661 agccgcgcaa cggtgtgttc cgcgagatgg cggccctggc cgatcaggcg cgtctggcgg
      721 cggccgcgcc gcggagggct ggcggcctgg atctttccgg ccgtcgattc tggaagatgg
      781 gcctgggcgc gatcggaacc gaggatcaca tctacgacga ggccatcgcg ggcgactatg
      841 tcgtcctggg ctggggcggt gaggtcgact ggagcgacgc ggcctatgag gacattggag
      901 cggttcgcgc caagtgggcc gaggttggac cggccgacag caagccgagc aatgtctctc
      961 agcttcaccc gttccgctcg gagatgaaga aaggcgacat catcatcgtc ccctatggca
     1021 acaccgcttt tagggcgatt ggcgaggtca cgggcgacta caccttcgtg gccaaccctg
     1081 acggcgagta caatcatcgt cgcgaggtca actggctcct caagcttgaa aagcccctgc
     1141 cgctggacag catcgtggag ggggccttca cgatgcgaac cctctatccg atcaatgcga
     1201 gccgcatccg gatggaagcg gtgtcgcggc tgattggcgc tccgggggac gcagaggcca
     1261 ctgcgccggg cgtggccccg tcgttcgtgc tggtgatcga cgagatcaat cgcgccaaca
     1321 tctccaaggt gtttggcgag ttgatcaccc tgcttgaggc cgacaagcgt ctcggcatgg
     1381 aaaacgaaat ccgggtcaag ctgccctatt ccggggagat gttcggcgtg ccggcgaacc
     1441 tccacctgat cggcaccatg aacacggccg accgctcgat cgcgcttctc g
//
LOCUS       DAOMEO010000914         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8653_length_1488_cov_2.205164, whole genome shotgun sequence.
ACCESSION   DAOMEO010000914 DAOMEO010000000
VERSION     DAOMEO010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8653_length_1488_cov_2.205164"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1114)
                     /locus_tag="PLH31_16830"
     CDS             complement(35..1114)
                     /locus_tag="PLH31_16830"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQR90894.1"
                     /translation="MDTTRRGLLIGGAALAPTLVAAETLPPPAIRAHLGVTENPYGPS
                     PAARRAAAGALEKTPYYAWSLEPPLVEAIARREGVSPDMVGLCNGSLEALSLLSTHFS
                     KTGPIVAPQPTYATPLTYAERQGAKLDWIPVTGDHQIDLPALAARARGAGCVYVCNPN
                     NPTGLLMDPDALRAFCMAASKQAPVIVDEAYVEISPDPVRHSMIDLVKAGHDVVVART
                     FSKVYGMAGLRVGFIIARPERVRQLKSLIPTHKGRPGLAAALACYGDATYLAGAKAYL
                     EGCRETIYAICKANGLGYLPSFGTYVFVDTGKPNLAFQKTLATMGVEVRVFDSARHPT
                     WIRVGTASPAELEVFAQVLPRALAV"
     gene            1395..>1488
                     /locus_tag="PLH31_16835"
     CDS             1395..>1488
                     /locus_tag="PLH31_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90895.1"
                     /translation="MTLSFWTRRKSIDAIAAGHDGHQLKKTLSWP"
BASE COUNT          244 a          484 c          527 g          233 t
ORIGIN      
        1 ggccggactg atcccccttg gccggaatca gggtctagac ggcgagcgcc cttggcagaa
       61 cctgggcgaa cacctccagc tcggccgggc tggccgtgcc gacccggatc caggtgggat
      121 gacgggcgct gtcgaacacc cgcacctcga ctcccatcgt cgccagggtc ttctggaacg
      181 ccaggttcgg cttgcccgta tcgacgaaca cataggtgcc gaacgacggc agatagccca
      241 gcccattggc cttgcagatc gcgtagatcg tctcgcggca gccttcgaga taggccttgg
      301 cgccggccag ataggtggcg tcgccatagc aggccagggc cgccgccagg ccaggcctgc
      361 ccttgtgggt cgggatcagg ctcttcagct gcctgacccg ttcgggccgg gcgatgatga
      421 acccgacccg caggccggcc atgccataga ccttcgagaa cgtgcgggcg acgaccacat
      481 cgtgaccggc cttgaccaga tcgatcatcg agtgacggac gggatcgggg ctgatctcga
      541 cataggcctc gtcgacgatc accggcgcct gcttcgacgc cgccatgcag aaggcgcgca
      601 gggcgtccgg gtccatcagc aggccggtcg gattgttggg attgcacaca tagacgcagc
      661 ccgcgccgcg ggccctggcg gcgagcgccg gcaggtcgat ctggtggtcg cccgtcaccg
      721 gaatccagtc cagtttcgcg ccctgccgct cggcataggt caggggcgtc gcataggtcg
      781 gctgcggcgc gacgatggga ccagtcttcg agaaatgggt cgacagcagc gacagcgctt
      841 ccagcgatcc gttgcacagg cccaccatgt cgggactgac gccttctcgc cgggcgatgg
      901 cctcgaccag cggcggctcc agcgaccagg cgtagtaggg cgttttctcc agcgccccgg
      961 cggcggccct gcgagcggca gggctgggac cgtaggggtt ctcggtaacg cccagatggg
     1021 cgcggatcgc cggcggcggc agggtttcgg cggcgacgag ggtgggagcc agggcggcgc
     1081 cgccgatgag caggcctcgt cgcgtcgtgt ccatgggtcg ctctccgcta tcggttctgc
     1141 agttgaggcg cgggcgatga caggatcaag acgtctgtcg ggtccgggcg atggcgggca
     1201 ggatctgccc tgtcctggag cggaaccgcc gttcagggac agtttgcggg caaacccgcc
     1261 cagaatcccc gcgatcgttc cgtctggcgg gccgggccga caatcttcgg caggaaatgc
     1321 ccccgccgat cgcgcacgtc ttggcgaaag gcgtcgccca acccatggtc gcggcggaga
     1381 cgaagtcgga gggaatgacc ttgagtttct ggacgcggcg caagtcgatt gacgccatcg
     1441 cggcgggcca tgacggccat cagctgaaga agaccctgag ctggccgc
//
LOCUS       DAOMEO010000915         1487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8659_length_1487_cov_2.316341, whole genome shotgun sequence.
ACCESSION   DAOMEO010000915 DAOMEO010000000
VERSION     DAOMEO010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8659_length_1487_cov_2.316341"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..562)
                     /locus_tag="PLH31_16840"
     CDS             complement(128..562)
                     /locus_tag="PLH31_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HQR90896.1"
                     /translation="MKRLAITALALSLMVGTAATAAPQRYDDRGNGRYEQNRYDDRRD
                     DRADRRDDRRDDRRDYRADRRDDRRDYRADYRDDRRDYRRWARGQRLDARYRGNGYYV
                     SDYHRHGLRAPPRGYRWQRVNDQYVMAAIATGLIASVIIANR"
     gene            737..1456
                     /locus_tag="PLH31_16845"
     CDS             737..1456
                     /locus_tag="PLH31_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQR90897.1"
                     /translation="MTLFPAFDPRRRLPALLRAARRELGATAALLIAALSALAFLSIA
                     EEVAEGETRAIDLAILDALRQPGQPRELIGPDWLHVAAIDITALGSLAVLGLLILLAF
                     ALLACLRQWGEGLLLLIGAGGGLAISQGLKLLFGRERPDMDYRAVEAVNASFPSGHAM
                     LSAVVFLTLGALAARFAQRKRLKALALGSAVALSLLVGLSRIYLGVHWTSDVVAGWCV
                     GATWAMACWLAAFAWTRWRSR"
BASE COUNT          191 a          481 c          533 g          282 t
ORIGIN      
        1 accagggtga tcagcggatc ggcggcggag ctggtttcgg cgaactcggg catttcaccc
       61 gattagccgg ttgacgtggg gtaggggaag ggggcgccgc atcgcgacgc ccccaccagg
      121 caacttttta gcggttggcg atgatcaccg aggcgatcag gcccgtggcg atcgcggcca
      181 tgacgtactg gtcgttcacg cgctgccagc gatagccgcg cggcggggcg cgcaggccgt
      241 ggcgatgata gtcggagacg tagtagccgt tgccgcgata gcgggcgtcc aggcgctggc
      301 cgcgagccca gcggcgatag tcgcggcggt cgtcgcggta gtccgcacgg tagtcgcggc
      361 ggtcgtcccg acggtcagcc cgatagtcgc gacggtcatc acggcgatca tcgcgacggt
      421 cggcgcggtc gtcccggcga tcgtcgtaac ggttctgttc gtagcgacca ttgccacggt
      481 cgtcatagcg ttgcggcgcg gcggtggcgg cggttcccac catcagcgac agggcaaggg
      541 cagtgatagc taggcgcttc atggcggtct ccttttcgat gaccccaacc tattgaagcg
      601 gacgtggccc ggacatgaac ggccctggtc agctcttgtt catgtttgat tagccatgcc
      661 gccgtggttg cacgcgacgc gttcgcccga tgtccctccg cgtggagcca agcttggcct
      721 tgcgcgttgg acgagcatga ccctgttccc tgcttttgat ccgcgtcggc gcctgcccgc
      781 cttgctccgg gccgcccggc gcgagctggg cgccaccgct gcgctcctga tcgccgccct
      841 gtcggccctg gccttcctgt cgatcgccga agaggtggcc gagggcgaaa cccgggccat
      901 cgacctggcc atcctcgacg ccctgcgaca gcccggccag ccgcgcgaac tgatcggccc
      961 cgactggctg catgttgcgg cgatcgacat taccgccctg ggctcgctgg cggtgcttgg
     1021 cctgctgatc ctgctggcct tcgcgctgct ggcctgtctg cggcagtggg gcgaagggct
     1081 gctgctgctg atcggcgccg ggggcggcct ggcgatcagc cagggcctga agctgctgtt
     1141 tggacgcgag cgcccggaca tggactaccg cgccgtcgag gcggtcaatg ccagcttccc
     1201 gtccggtcac gccatgctgt cggccgtggt cttcctgacc ctgggcgccc tggccgcgcg
     1261 gtttgcgcag cgcaagcgac tcaaggccct ggcgctgggt tcggcggtgg cgctgtcgct
     1321 gctggtcggt ctcagccgga tctatctggg cgttcactgg accagcgacg tggtggccgg
     1381 ctggtgcgtg ggcgcgacct gggccatggc ctgctggctt gcagcgtttg cctggacgcg
     1441 ctggcggtcg cgctagagca ttttccgata agtgtgaaac ggttatc
//
LOCUS       DAOMEO010000916         1485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8674_length_1485_cov_2.688112, whole genome shotgun sequence.
ACCESSION   DAOMEO010000916 DAOMEO010000000
VERSION     DAOMEO010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8674_length_1485_cov_2.688112"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="PLH31_16850"
     CDS             complement(<1..159)
                     /locus_tag="PLH31_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR90898.1"
                     /translation="MRAPFTRETADVRRQALVAAAETVLARDGVAGASVRSICAEAGV
                     SPGLLRHYF"
     assembly_gap    309..318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<319..1457)
                     /locus_tag="PLH31_16855"
     CDS             complement(<319..1457)
                     /locus_tag="PLH31_16855"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQR90899.1"
                     /translation="MNAPTSNLDPQADWGLPGWTYGNDRFFKEEQDRILRPSWQVVCH
                     LNDIPKAGDFHTLDFLSESVVVVRAKDGGVRAFTNVCRHRGARLVDGPSGSCGKGGGR
                     IVCPYHAWTYDLEGRLIGVPMRDTYPALDMDRQGLVKVDVEIWRGFVFVRLEGDGPSV
                     AEMMAPYDHEVAHYRFEELQPFGRVTLRPRTVNWKNISDNYSDGLHIPIAHPGLTRLF
                     GKGYGVEAETWVDKMWGQLIDEPSANPSERLYQDILPDVPHLPPERKRLWTYFKLWPN
                     FAFDIYPDQVDFMQFIPVSATQTLIREIAYALPDDRREMKAARYLNWRINRQVNAEDT
                     ELVARVQEGMASRSFTAGPLASSEVSLRSFGRKMRALIPEARLDRP"
BASE COUNT          237 a          475 c          511 g          252 t
ORIGIN      
        1 gaaatagtgc cgcaacaggc cgggcgagac gcccgcttcc gcgcagatgc ttcttacgga
       61 tgctccggcc accccgtcgc gagccaggac ggtttcggcg gccgcgacca gggcctggcg
      121 gcggacgtcg gcggtctcgc gggtgaaggg ggcgcgcatg gtcctagccc tcccccttga
      181 tgggggaggg ttgggtgggg gggatcgggc ggtggcggtt gaaccgctgc atcaccccca
      241 tccccgaccc ttcctccatc gagggggaag ggagatgtct gggcatcacc accccgtcgg
      301 cggccggtnn nnnnnnnngg ccggtccagc cgcgcttcag ggatcagcgc ccgcatcttc
      361 cggccaaaac tgcgcagcga aacctccgag ctcgccagcg gccccgccgt gaagctccgc
      421 gacgccatgc cctcctgcac ccgggccacc agctcggtgt cctcggcatt gacctgacgg
      481 ttgatgcgcc agttcagata gcgggccgcc ttcatctccc gacggtcatc gggcagggcg
      541 taggcgatct cgcggatcag ggtctgggtg gccgagaccg ggatgaactg catgaagtcc
      601 acctggtcgg gatagatgtc gaaggcgaag tttggccaca gcttgaaata ggtccacagg
      661 cgtttgcgct cgggcggcag gtgcggaacg tcgggcaaga tgtcctggta cagccgctcg
      721 gacgggttgg ccgacggctc gtcgatcagc tggccccaca tcttgtcgac ccaggtttcg
      781 gcttccacgc catagccctt gccgaacagc cgggtcaggc cggggtgggc gatggggata
      841 tgcaggccgt ccgaatagtt gtcgctgatg ttcttccagt tcaccgtccg gggccgcagg
      901 gtgacgcggc cgaagggctg aagctcttcg aagcgatagt gggccacctc gtgatcgtag
      961 ggcgccatca tctcggcgac ggacgggcca tcaccttcca gccgcacgaa aacgaagccg
     1021 cgccagatct cgacatcgac cttgaccagg ccctgcctgt ccatgtcgag cgccggatag
     1081 gtgtcgcgca tgggtacgcc gatcaggcgg ccttccaggt cgtaggtcca ggcgtgatag
     1141 gggcagacaa tgcggccgcc gcccttgcca cagctgccgc tggggccgtc gaccagccgc
     1201 gcgccgcgat ggcggcagac attggtgaag gcccgaacgc cgccgtcctt ggcgcgcacc
     1261 accacgacgc tctcgcttag gaagtccagg gtgtggaagt cgccggcctt ggggatgtcg
     1321 ttcaggtggc agaccacctg ccacgagggg cgcaggatcc tgtcctgttc ctctttgaag
     1381 aaccggtcgt tgccgtaagt ccagccgggc aggccccagt ctgcttgcgg gtcgaggttg
     1441 gaggtggggg cgttcatgcg ccgagccctc cagcaagatc acttt
//
LOCUS       DAOMEO010000917         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8687_length_1483_cov_2.824230, whole genome shotgun sequence.
ACCESSION   DAOMEO010000917 DAOMEO010000000
VERSION     DAOMEO010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_1483_cov_2.824230"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1483
                     /locus_tag="PLH31_16860"
     CDS             52..>1483
                     /locus_tag="PLH31_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR90900.1"
                     /translation="MPRHIVVEDARIDTRGDILKLDIYSGQTERLMRGSDRFGGFQLD
                     LKGEIRTRQELGYDKGAVYLAQWIRDPSSGEWSEHFRWYAKDREPVSIVGFTEDPNII
                     YVSSAKGRDKSGLYVYDIKARQILEPLLEHRLFDAGGIVTSSAKADYGRVLGFSYAGP
                     TNSIYWTDETFSALNKSLRTALGVKTTPINWVDPATGTATRLPVADGADASITDWSED
                     LKHFIVQKSGPANPGEYYLLDSSGALRLLGKVRPDLDAASLGVTRLVQYPARDGLMIP
                     GFLTTPPADQFGPGPYPTLIEPHGGPWSRDNLDWDPAGWVQYFASRGYAVLQPQFRGS
                     EGWGQKLWRAGDGEWGQKMQDDKDDGAQWLIDQKIAAPDRIAMFGYSYGGYAALAAAI
                     RPNGLYQCAISGAGAGDLASLKQATFENRFQREFQNPTIRGMDALERAKEAQIPLLIY
                     HGDRDTNVEVKQSRKFVDALKAADKPH"
BASE COUNT          265 a          498 c          478 g          242 t
ORIGIN      
        1 gctgccactt ttgcgacagc tgctgtgtca tgccaagctg agccatatcg gatgccgcgt
       61 cacatcgtgg tggaggatgc gcgcatcgat acgcgcggcg acatcctcaa gctggacatc
      121 tattcgggcc agaccgagcg gctgatgcgg ggctccgatc gctttggcgg cttccagctg
      181 gacctgaagg gcgaaatccg cacgcgtcag gaactgggct atgacaaggg cgcggtctat
      241 ctggcccagt ggatccgcga tccgtccagc ggcgagtggt ccgaacattt ccgctggtac
      301 gccaaggacc gtgagcccgt ctccatcgtc gggttcaccg aagaccccaa catcatctat
      361 gtctcctcgg ccaagggtcg cgacaagtcg ggcctctatg tctacgacat caaggcgcgg
      421 cagattctcg agccgctgct ggagcacagg ctgttcgacg ccggcggcat cgtcacctct
      481 agcgccaagg ccgactatgg ccgggttctg ggcttttcct atgcgggccc gaccaacagc
      541 atctactgga cggacgagac cttctcggcc ctgaacaaga gcctgcgcac ggccctgggc
      601 gtgaagacca caccgatcaa ctgggtggat ccggcgaccg gaaccgcgac gcgcctgccg
      661 gtggccgacg gcgccgatgc ctcgatcacc gactggtcgg aagacctcaa gcacttcatc
      721 gtccagaaat caggaccggc caatccgggc gagtactacc tgctcgactc gtcgggcgcg
      781 ctgaggctgc tgggcaaggt gcggcccgac ctggacgccg cctcgctggg cgtcacgcgc
      841 ctggtgcagt atcccgcccg cgacggcctg atgatcccgg ggttcctgac cacgcccccc
      901 gccgaccagt ttggtcccgg cccctaccca accctgatcg agccgcatgg cgggccgtgg
      961 tcgcgcgata atctggactg ggatccggcc ggctgggttc agtacttcgc cagccgcggc
     1021 tacgccgtcc tgcagcccca gttccggggc tccgaaggct ggggccagaa gctgtggcgc
     1081 gccggcgacg gcgagtgggg ccagaagatg caggacgaca aggacgacgg cgcccagtgg
     1141 ctgatcgacc agaagatcgc cgcgccggac cgtatcgcca tgttcggtta ttcctatggc
     1201 ggctatgccg ccctggccgc cgccatccgg cccaatggcc tctatcagtg cgcaatctcg
     1261 ggcgcggggg ccggcgacct ggccagcctg aagcaggcga cgttcgagaa ccgtttccag
     1321 cgcgagttcc agaacccgac aatccggggc atggacgctc tggagcgcgc caaggaagcc
     1381 cagatcccgc tgctgatcta tcatggggac cgcgacacga acgtcgaggt caagcagtca
     1441 cgcaagttcg tcgacgcgct gaaggcggcc gacaagccgc atc
//
LOCUS       DAOMEO010000918         1483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8688_length_1483_cov_2.700280, whole genome shotgun sequence.
ACCESSION   DAOMEO010000918 DAOMEO010000000
VERSION     DAOMEO010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8688_length_1483_cov_2.700280"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1271
                     /locus_tag="PLH31_16865"
     CDS             45..1271
                     /locus_tag="PLH31_16865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR90901.1"
                     /translation="MSTPEAVGLSSERLKRIETFIQDKYIASGKLPCALTQVWRRGQL
                     AYTSVLGSADIERGKPLQADSLFRIYSMTKPITSVAFMMLVEEGKVSLDDPVHRFIPA
                     WRDLGVFQAGVAGAFQTQRASEPMRMLDLLRHTAGLTYGFQQRTNVDAAYRKLKLDGV
                     DSEGGLQDFVDRLATVPLEFSPGAAWNYSVATDVLGYLVETISGQPFDAFLKSRIFDP
                     LKMVDTSFFVPESQRARFTACYALTPSGKIVLQDDPEKSRYLSDPAVKSGGGGLVSTA
                     DDYMRFCRMLLNGGELDGARLLSPKTIKLMTINHLPGGKELTQLSKSLFSEAVFEGLG
                     FGLGFAMTIDQARTKNLGSHGEYFWGGMASTAFWVDPVEDLAVVFLTQLMPSTAYPIR
                     RELRTLVYSAFTESAA"
BASE COUNT          256 a          499 c          458 g          270 t
ORIGIN      
        1 agaaacgccg ccagaacccg ggtggtcgca gggagaatca gatcttgagc acgcctgaag
       61 ccgtaggcct gtcgtccgag cgcctgaagc gcatcgaaac cttcatccag gacaaataca
      121 tcgccagcgg caagctgccc tgcgccctga cccaggtctg gcggcgcggc cagctggcct
      181 acacctccgt gctgggctcg gctgatatcg agcgcggcaa gccgctgcag gccgacagcc
      241 tgtttcgcat ctattcgatg accaagccca tcaccagcgt cgccttcatg atgctggtcg
      301 aggaggggaa ggtttcgctg gatgatccgg tccatcgctt cattccggcc tggcgcgacc
      361 ttggcgtctt tcaggccggc gttgctggtg cgttccagac ccagcgtgcg tctgagccca
      421 tgcggatgct ggacctgctg cgccacaccg ccggcctgac ctatggcttc cagcagcgca
      481 ccaatgtcga tgcggcctat cgcaagctga agctggacgg cgtcgacagc gagggcggtt
      541 tgcaggactt cgtcgacagg ctggccaccg tgccgctgga gttcagcccc ggcgcggcct
      601 ggaactattc ggtggccacc gacgtgctgg gctatctggt cgagacgatc tccggtcagc
      661 ccttcgacgc cttcctcaag agccgcatct tcgatccgct gaagatggtc gacaccagct
      721 tcttcgtccc ggaaagccag cgcgcccgct tcacggcctg ctacgccctg acgccgtcgg
      781 gcaagatcgt cctgcaggac gacccggaaa agagccgcta tctgagcgat ccggcggtga
      841 agtcgggcgg cggcggcctg gtctccacgg ccgacgacta tatgcgcttc tgccggatgc
      901 tgctgaacgg cggcgagctg gacggcgcgc ggttgctcag ccccaagacc atcaagctga
      961 tgaccatcaa ccacctgccg ggcggcaagg agctgaccca gctgtccaag tcgctgttca
     1021 gcgaggcggt gttcgagggg cttggctttg gccttggctt cgccatgacc atcgatcagg
     1081 cgcgcaccaa gaaccttggc tcgcacggcg aatatttctg gggcggcatg gcctcgacgg
     1141 cgttctgggt cgacccggtc gaagaccttg cggtggtgtt cctgacccag ctgatgccat
     1201 cgaccgctta cccgatccgg cgtgaactgc ggaccctggt ctattcggcg ttcacggagt
     1261 cggccgccta ggatccgccc actctgctgg agccgctggc ccacacaaga aagcggcgcg
     1321 ggagctcggc tcccgcgcca aaggtggctt tacctagttc gacgcattgg ccacaacacg
     1381 ccgcgcccgc gtaaaatccc tgacttcgaa tttgtggaac gcttgcaagt cgcgtcccaa
     1441 gccccagctt ccacgcgcac gccctccccc agcacggatc gtt
//
LOCUS       DAOMEO010000919         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8712_length_1480_cov_2.040702, whole genome shotgun sequence.
ACCESSION   DAOMEO010000919 DAOMEO010000000
VERSION     DAOMEO010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8712_length_1480_cov_2.040702"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLH31_16870"
     CDS             complement(<1..763)
                     /locus_tag="PLH31_16870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HQR90902.1"
                     /translation="MAASDGSQQATSPSYRLAALDQAFLLGDSMRGVRFLLEFAKADE
                     ALRAWGIRSTIVVFGSARVREDTAGRPGDWYAQARLFGRIASEQGGACGPAGQPLDNV
                     IATGGGPGIMEAANRGAFETGAPSIGFNITLPREQLPNPYSTPDLTFRFHYFAMRKMH
                     LAMRANALVVFPGGFGTLDELFELLTLRQTRKAPAIPIVLVDETYWRSVINFEALIEQ
                     GMVDAADLSLFAFADTADAAWTQLVSAGLCLSRPAD"
     gene            complement(772..1371)
                     /locus_tag="PLH31_16875"
     CDS             complement(772..1371)
                     /locus_tag="PLH31_16875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQR90903.1"
                     /translation="MKHAVILAHPKPTSFCAAIAKTCVEHLRATGHAVVMRDLYGLDF
                     DPRLKAEELPGPLGAQAAPDIIAERQALGDVDSFIFVYPFWFNAPPAILKGYVDRVFS
                     MGFGYAAGAGGAESLLDDKTLLSFSTSGAPEDWVKTTGALKALMTVFDLHLSGVCGLR
                     VLDHKHFGSIVSNTTAESGADVMEEVRSRLDDLFGSAPA"
BASE COUNT          280 a          459 c          510 g          231 t
ORIGIN      
        1 ggtccgcagg tcgagacagg cacaagccgg cgctgacgag ctgtgtccac gcggcgtcag
       61 cggtgtcggc aaaagcgaaa agcgaaagat cggcggcgtc gaccatcccc tgttcgatca
      121 gggcctcgaa gttgatcacc gaacgccagt aggtctcatc gaccaggacg atcgggatcg
      181 ccggagcctt tcttgtctga cgcagggtca ggagctcgaa gagctcgtcc agggtgccga
      241 accccccggg aaacaccacc agagcgttgg cccgcatcgc cagatgcatc ttgcgcatcg
      301 cgaaatagtg aaaccggaag gtcagatccg gcgtggaata ggggttggga agctgctccc
      361 tgggcagggt gatgttgaag ccgatcgacg gagcgccggt ctcgaaggcg ccgcgattgg
      421 ccgcttccat gatcccgggg ccgccgccgg ttgcgatgac attgtcgagg ggctggccgg
      481 ctggcccgca cgcgccgcct tgctcggagg cgatacgccc aaaaagccgc gcctgggcgt
      541 accagtctcc cggacgtccg gccgtgtcct cgcgcacccg agcgctgccg aagacgacga
      601 tggtcgagcg tatgccccag gcccttagcg cctcatcggc cttggcgaac tccagcagaa
      661 agcgcacgcc gcgcatggag tcgccgagaa ggaaggcctg gtccagcgcc gcgagccggt
      721 aggacgggga ggtggcctgc tgactaccgt ccgaggcggc cagatcagcc gtcacgccgg
      781 ggctgagccg aaaaggtcgt ccagacgcga gcgcacttcc tccatgacgt cggcgccgga
      841 ctcggcggtg gtgttggaaa caatcgagcc aaaatgtttg tggtccagaa cccgtaggcc
      901 gcagactccc gagaggtgca ggtcaaacac tgtcatcagg gccttcaggg ccccggtggt
      961 cttcacccag tcctcgggcg cgccggatgt cgaaaagctc agcagggtct tgtcgtcgag
     1021 cagcgactcc gcgccgccgg ctccggccgc atagccaaag cccatgctga acacgcggtc
     1081 cacatagccc ttgagaatgg cggggggtgc gttgaaccag aacggataga caaagatgaa
     1141 gctgtccaca tcgcccagcg cctgtcgctc ggcgatgatg tccggggcgg cctgcgcgcc
     1201 cagcggtccg ggcagttcct cggccttgag gcgaggatcg aaatccagcc catagaggtc
     1261 ccgcatcacc acggcgtggc cggtggcgcg caggtgctcg acgcaggtct tggcgatggc
     1321 cgcgcagaag ctggtgggct tgggatgggc gaggatgacg gcgtgtttca tgggagccag
     1381 gctgcgcctt ctgtcgatgg cgacagacta gccgggcgcg cgagcctcga attgattcag
     1441 atcagggaac atgacgcaga cgcgccgatc ttggagcttc
//
LOCUS       DAOMEO010000920         1480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8714_length_1480_cov_1.701754, whole genome shotgun sequence.
ACCESSION   DAOMEO010000920 DAOMEO010000000
VERSION     DAOMEO010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8714_length_1480_cov_1.701754"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLH31_16880"
     CDS             complement(<1..269)
                     /locus_tag="PLH31_16880"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQR90904.1"
                     /translation="MSDIVIVSAARTPVGSFNGALSSLPASELGKIAIQAAVERAGIS
                     PADVDEVILGQVLQAGAGQGPARQAAINAGIPVGSPAWSLNQLCGS"
     gene            488..1171
                     /gene="phaR"
                     /locus_tag="PLH31_16885"
     CDS             488..1171
                     /gene="phaR"
                     /locus_tag="PLH31_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQR90905.1"
                     /translation="MSENPEKGETTAGAEKAAGQRVIIKKYANRRLYNTASSSYVTLE
                     HLSDMVKEGVDFVVYDAKSNDDITRSVLTQIIFEEENRGGGQNLLPIQFLRQLIGFYG
                     NSMQAFLPSYLEMSLESFTKQQERMRTQFAGVTPGLTAAKLPMGVGVYEEQIRQNMAL
                     FDRAMKMFSPFAYARPEDAAAAAAAASNAPDPTPAAPAPQPSEDSLADLKRQLEVMQQ
                     QIEKLATKP"
     gene            1232..>1480
                     /locus_tag="PLH31_16890"
     CDS             1232..>1480
                     /locus_tag="PLH31_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90906.1"
                     /translation="MNGPIDPIRRSSPSRRALTVRQNTAEHDARETEPEDVVFVREEQ
                     PAPPPRKSGFATFAAHLMGQSGQKRGLRGGQEVLDSARS"
BASE COUNT          292 a          503 c          458 g          227 t
ORIGIN      
        1 gaaccgcaca gctggttgag gctccaggcg gggctgccca ccgggatgcc ggcattgatc
       61 gcggcctgac gggcgggacc ctggccggcg ccggcctgca gcacctgacc cagaatcact
      121 tcgtccacgt cggccggcga gatacccgcc cgctcgacgg cggcctggat ggcgatcttg
      181 cccagttcgg acgcgggcag gctggacagc gccccgttga aggagccgac cggcgtgcgg
      241 gcggcggaaa cgatgacgat gtcgctcatg gcgagtctcc cgtaacgatc tgacgtgacg
      301 ccttcgcagt tgcggcgccc gtgctctttt gcagtgcagc agcacaatgc ataccgaacc
      361 gcgttcgtca cgggcacatt ttcacggctt gttgcggctt ccaagtgcct gaccactggc
      421 gccatgcctt tgcattcgcg atagtgcgat gcgaaaagcg ggcgacaccg ctgtcctagg
      481 gaacgaaatg tccgagaacc ctgaaaaagg cgaaaccacc gccggcgccg aaaaagcggc
      541 cggtcaacgc gtgatcatca agaaatacgc caaccgtcgc ctctacaaca cagcgtccag
      601 ctcgtacgtc accctggaac acctgtccga catggtgaag gagggcgtcg acttcgtggt
      661 ctatgacgcc aagtccaacg acgacatcac ccgttcggtc ctgacccaga tcatcttcga
      721 ggaagaaaac cggggcggcg gacagaacct gcttccgatc cagttcctgc gccagctgat
      781 cggcttctac ggcaattcca tgcaggcctt cctgcccagc tatctggaaa tgtcgctgga
      841 aagcttcacc aagcagcagg agcgcatgcg cacccagttc gcgggcgtca cgccgggcct
      901 gaccgccgcc aagctgccga tgggcgtggg cgtgtatgaa gaacagatcc gccagaacat
      961 ggccctgttc gaccgggcga tgaagatgtt ctcgcccttc gcctatgccc ggccagaaga
     1021 cgccgccgca gcggcggcgg cagcctcgaa cgcgccggac ccgactccgg ccgcccccgc
     1081 cccgcagccc agcgaggatt ccctggccga cctgaagcgt cagctcgagg tcatgcagca
     1141 gcagatcgag aagctggcga ccaagcctta aagagctgga aaccacgccg gaacagccgt
     1201 tcttaacggt cggagcccta gggtcttcac catgaacggc cctatcgatc ccattcggcg
     1261 ttccagccct tcgcgtcgcg ccctgacggt gcgccagaac acggccgaac acgacgcccg
     1321 ggaaaccgaa ccggaggacg tcgtgtttgt ccgcgaggag caaccggccc cgccgccccg
     1381 caagagcggc tttgcgacct tcgccgccca cctgatgggc cagagcggcc agaagcgcgg
     1441 cctgcgcggt gggcaggaag tgctcgactc ggcgcgatcg
//
LOCUS       DAOMEO010000921         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8720_length_1479_cov_2.186798, whole genome shotgun sequence.
ACCESSION   DAOMEO010000921 DAOMEO010000000
VERSION     DAOMEO010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8720_length_1479_cov_2.186798"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..>234)
                     /locus_tag="PLH31_16895"
     CDS             complement(96..>234)
                     /locus_tag="PLH31_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR90907.1"
                     /translation="SGQDVLVLAHGFFNWMVARSLKKKGARQLVDEGHAYWSVKRFAL
                     A"
     assembly_gap    235..244
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<245..689)
                     /locus_tag="PLH31_16900"
     CDS             complement(<245..689)
                     /locus_tag="PLH31_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR90908.1"
                     /translation="MADASNADHILAEAKPVRPGVITLARHGEPALSRKVNFNAQAYG
                     EWWALYEVGGLKAGQTPPESLVEACRQASVIIASTRRRSIETAQAVCGGRAFATDPLF
                     IEAPLPPPPWPAWIKMKPSHLGFFARFWWWFFNHHGGQETRAEAEI"
     gene            complement(725..>1479)
                     /locus_tag="PLH31_16905"
     CDS             complement(725..>1479)
                     /locus_tag="PLH31_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQR90909.1"
                     /translation="LVHPQAFIDTILASAPHTGRHALDPDTILTPGSLVAARRAAGAA
                     VSAVRQIAAGEGSRAFCAVRPPGHHAEPGTAMGFCVFSNVAVAARVAQASGLRRVAII
                     DFDVHHGNGTQAAFENDPTVFFASIHQSPLYPGTGDPSETGLGNIANAIVAPGASSQT
                     WRAGFEGLMDQVDGFSPDLILVSAGFDAHVRDPLSEQNLEAEDFAWATRAIVSVANAR
                     SRGRIVSSLEGGYDLHALGRSAAAHVSALQEG"
BASE COUNT          243 a          481 c          531 g          214 t
ORIGIN      
        1 caccctagtg gggcgggaag gtgcgggcaa cgacctagaa tgtggccgtg gttaagggaa
       61 gccggaatga gtcctccgtc ttggggctga cggcttcagg ccagggcgaa gcgcttgacg
      121 ctccaatagg cgtggccttc atcgaccagc tggcgcgcgc ccttcttctt cagtgaccgg
      181 gccaccatcc agttgaagaa gccgtgggcc agcaccagga catcctgacc gctgnnnnnn
      241 nnnnggattt cggcctcggc gcgggtctcc tgcccgccgt ggtgattgaa gaaccaccac
      301 cagaagcggg cgaagaaccc caggtggctg ggcttcatct tgatccaggc cggccagggc
      361 ggcggcggca gcggagcctc tatgaaaagc ggatcggtgg cgaaggcccg accgccgcaa
      421 accgcctggg cggtctcgat cgaccggcgg cgggtcgagg cgatgatgac gctcgcctga
      481 cggcaggcct caaccaggga ttcggggggt gtctggccgg ccttcagtcc gccgacctca
      541 taaagggccc accactcgcc ataggcctgg gcgttgaaat tcaccttgcg cgacagggcc
      601 ggctcgccgt gacgggccag ggtgatgacg cctggccgca cgggcttggc ctcggcaagg
      661 atgtggtcgg cgttcgatgc gtcggccatg ttcgcgaggg agcgacggtc gcccgcctgt
      721 cccttcatcc ctcctgcaga gcgctgacat gcgcggcggc tgagcgcccc agcgcgtgta
      781 ggtcgtagcc gccctcgagc gaggagacaa tacggccccg cgaccgggcg ttggcgaccg
      841 agacgatggc ccgggtcgcc caggcgaaat cctcggcctc caggttctgc tcagacagcg
      901 gatcgcggac atgggcgtcg aaaccggccg agaccaggat cagatcgggg gagaacccgt
      961 ccacctgatc catcagcccc tcgaagccgg cccgccaggt ctggctggaa gccccgggcg
     1021 cgacgatggc gttggcgata ttgcccaggc ccgtctcgga cgggtcgccg gtgccgggat
     1081 agagcggcga ctgatggatc gaggcgaaga agaccgtggg gtcgttttcg aacgccgcct
     1141 gggtgccatt gccgtggtgg acgtcgaaat cgatgatggc tacgcggcgc aggcccgagg
     1201 cctgggccac ccgcgccgcc accgcgacat tggaaaagac gcaaaagccc atggccgttc
     1261 ccggctcggc gtggtggccc ggcggccgca ccgcgcagaa ggctctgctg ccctccccgg
     1321 ccgcgatctg ccgcaccgcc gaaaccgccg ccccggccgc gcgccgcgcc gcgaccagac
     1381 tgccgggggt caggatggtg tcgggatcca gcgcatggcg accggtgtgc ggggccgagg
     1441 ccagaatggt gtcgatgaag gcctgcgggt gcaccaggg
//
LOCUS       DAOMEO010000922         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8722_length_1479_cov_1.719101, whole genome shotgun sequence.
ACCESSION   DAOMEO010000922 DAOMEO010000000
VERSION     DAOMEO010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8722_length_1479_cov_1.719101"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..659
                     /gene="folE"
                     /locus_tag="PLH31_16910"
     CDS             33..659
                     /gene="folE"
                     /locus_tag="PLH31_16910"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQR90910.1"
                     /translation="MPSALTSVAADRIEAAPGRPTQAQAEDAVRLLIAWAGDDPGREG
                     LIDTPARVARSYAELFSGYSEDPRTHLTRTFSETGGYDDLIVLSNIRVVSCCEHHMLP
                     VIGRAHVGYLPGNRVVGISKLARVVRGFSRRLQIQEKLTTDIANAIQDSLEPRGVGVV
                     IEAEHSCMTLRGVDTPGSMLTTSRLLGQMRDDPQTREEFLSLVRQARV"
     gene            762..1118
                     /locus_tag="PLH31_16915"
     CDS             762..1118
                     /locus_tag="PLH31_16915"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 family protein"
                     /protein_id="HQR90911.1"
                     /translation="MPGWSGRRPAPSRSTRWPPTPPSSSRSSRAPVWASPSPRRPATT
                     TLCLSMAPPLAAWALVLLPIMSGLRLVLLALALLAHAAWDARADLAPRWYAGLRWRLT
                     FGAMTGLLAGAVVLHD"
     gene            1111..>1479
                     /locus_tag="PLH31_16920"
     CDS             1111..>1479
                     /locus_tag="PLH31_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C-factor"
                     /protein_id="HQR90912.1"
                     /translation="MTEPASIAVVVSVTGGLGGALAKTLGAQARYDKVVGLARRRPDD
                     WPDSPDWPFITADVLKEQDLAAAAKVLSALGALDRVVVATGLLHGEGVTPEKTMRSLN
                     ASTLAELFAVNAVAPALVAKH"
BASE COUNT          206 a          507 c          503 g          263 t
ORIGIN      
        1 gtcgtacaca aaaatgtcgc atggagatcg ctatgccttc agccctgacg tcagtggccg
       61 cggatcgcat cgaggcggcg cctggccgtc ccacccaggc tcaggccgag gacgccgtcc
      121 ggctgctgat cgcctgggcc ggcgatgatc ccggccgcga aggcctgatc gacaccccgg
      181 cccgcgtggc acgctcctac gccgagctct tcagcggcta tagcgaggac ccacgaacgc
      241 acctgacccg cacgttcagc gagacgggcg gttatgacga cctgatcgtc ctttccaaca
      301 ttcgggttgt cagctgctgc gagcaccaca tgctgccggt cattggccgt gctcatgtgg
      361 gctatctgcc gggaaatcgg gtggtgggga tctccaagct ggcccgcgtg gtgaggggct
      421 tctcccggcg gctgcaaatt caggaaaagc tcaccacgga tatcgccaac gccatccagg
      481 acagtctgga gccccggggc gtgggcgttg tcatcgaggc cgagcacagc tgcatgaccc
      541 tgcgcggcgt cgatacgccc ggttcgatgc tgaccaccag ccgcctgctg gggcagatgc
      601 gagacgatcc ccagacccgt gaggaattcc tgagtctggt tcgtcaggcc cgggtctagt
      661 gcgctcagcg actgacaacg cgcctgcggt ggcgccccag gctccactgg ccttgaccgt
      721 cctgggcgcg ctcggcgtcg cgccgttctg gctgcccgtt cttgccgggg tggtctggcc
      781 gcagaccagc gccgtcgcgt tcgacgcgct ggccgcctac gccgccatca tcctctcgtt
      841 cctcgcgggc tcccgtctgg gcatcgccat caccgaggcg ccccgccaca accaccctct
      901 gcctgtccat ggcgccgccg cttgcggcct gggccctggt tctgctgccg atcatgtcgg
      961 ggctcaggct tgtgcttctt gctctggccc tgctggcgca tgcggcctgg gacgcccgcg
     1021 cggacttggc gccccgctgg tacgccgggc tgcggtggcg tctgacgttc ggcgccatga
     1081 cgggattgct ggctggcgcg gtggttctgc atgactgagc cggcttcgat cgcggtcgtc
     1141 gtcagcgtca ccggcgggct gggcggggcc ctggccaaga cacttggcgc gcaggcccgc
     1201 tatgacaagg tggtcggcct ggccaggcgg cgacccgacg attggccaga cagcccggac
     1261 tggccgttca tcaccgcgga cgttctgaag gagcaagacc tggcggcggc ggccaaggtc
     1321 ctgtccgcct tgggggcgct cgatcgggtg gtggtcgcca ccgggctgct gcacggtgaa
     1381 ggcgtcacgc ccgaaaagac catgcgatcg ttgaacgcct cgacgctggc ggagctcttc
     1441 gcggtcaacg cggtggctcc ggcgcttgtg gccaaacat
//
LOCUS       DAOMEO010000923         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8724_length_1478_cov_2.595221, whole genome shotgun sequence.
ACCESSION   DAOMEO010000923 DAOMEO010000000
VERSION     DAOMEO010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8724_length_1478_cov_2.595221"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLH31_16925"
     CDS             complement(<1..377)
                     /locus_tag="PLH31_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQR90913.1"
                     /translation="MSEIQSDESGAGKNGTGAKRSPLKWAIGAVMLVGVAAVLYVIAA
                     ASFKPSGPANLNEFKRASLAKLEVPASPRAAPGTTFFDEAGKPVTLADFKGQVVVMNL
                     WATWCAPCKKEMPTLAKLAAAYAA"
     gene            378..>1478
                     /gene="argH"
                     /locus_tag="PLH31_16930"
     CDS             378..>1478
                     /gene="argH"
                     /locus_tag="PLH31_16930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQR90914.1"
                     /translation="MTDNASKKPDAAEGAKGQAMWGGRFSAKPAELMQAINVSIGFDK
                     RLWAQDLAGSRAHARMLITQGVIASSDGEEILEGLARVEDELLTGTFPFRDEYEDIHM
                     NIEARLRELIGPVAGRLHTARSRNDQVALDFRLWVREACDRSAAQLDALQRALLAQAE
                     TYADALMPGFTHLQPAQPVTFGHHLMAYVEMFGRDASRFRDARARMNECPLGAAALAG
                     SPFPIDRHATAAALDFDRPTANSLDSVSARDFALEALSAASITATHLSRLAEEIVLWT
                     TPMFGFIKLTDAFTTGSSIMPQKKNPDAAELIRAKVGRILGSLTTLTVVMKGLPLAYS
                     KDMQEDKVPTFEAFDALELSLLAMAGMVADLTP"
BASE COUNT          248 a          512 c          465 g          253 t
ORIGIN      
        1 gccgcatagg cggcggccag cttggccagg gtcggcatct cctttttgca aggggcgcac
       61 catgtggccc agaggttcat caccaccacc tgccccttga aatcggcaag ggtcaccggc
      121 tttccggcct cgtcgaagaa ggttgtgcct ggcgcggcgc ggggcgaagc gggcacctcc
      181 agcttggcca gcgacgcccg cttgaattca ttgaggttgg ccggccctga gggtttgaac
      241 gaagcagcgg cgatgacgta tagaaccgcc gcaacgccga ccagcatgac ggcgccgatc
      301 gcccacttca gcggactgcg ctttgcgccc gttccgttct tgcctgcccc gctctcgtcc
      361 gactggattt cgctcatatg accgacaacg cctccaaaaa acccgacgcc gccgagggcg
      421 cgaagggtca agccatgtgg ggcggcaggt tctcggccaa accggcggag ctgatgcagg
      481 cgatcaatgt cagcatcggc ttcgacaagc gcctatgggc gcaggatttg gcgggctccc
      541 gcgcccatgc ccggatgttg atcacccaag gtgtgattgc aagtagcgat ggcgaggaaa
      601 tcctcgaagg cctggccagg gtcgaggacg aactgctgac cggaaccttc ccgttccgcg
      661 acgagtatga agacatccac atgaatatcg aggcccggct gcgtgagctg atcggcccgg
      721 tggccggccg cctgcacacg gctcggtcgc gcaacgacca ggtggcgctc gatttccggc
      781 tgtgggtccg cgaggcctgt gaccgttcgg ccgcccaact cgacgccctg cagcgcgctc
      841 ttctggccca ggcggaaacc tatgccgacg ccctgatgcc aggcttcacc catctgcagc
      901 ccgctcagcc ggtgaccttt ggccatcacc tgatggccta tgttgaaatg ttcggccgcg
      961 acgccagccg gttccgcgac gcccgggccc ggatgaacga gtgcccgctg ggcgccgcgg
     1021 ctctggccgg ttcgcccttc cccatcgacc gccacgccac ggccgccgcc ctcgacttcg
     1081 accgtccgac cgccaattcg ctggacagcg tctcggcccg cgattttgcg ctggaggccc
     1141 tgtcggctgc ctcgatcacc gccacgcatc tgtcgcgcct ggccgaggag atcgtgctgt
     1201 ggacgacgcc gatgttcgga ttcatcaagc tgaccgacgc cttcacgacc ggcagctcga
     1261 tcatgccgca gaagaagaac ccggacgccg ccgagttgat ccgcgccaag gtcggccgca
     1321 tcctgggcag cctgaccacc ctgaccgtgg tcatgaaggg cctgccgctg gcctattcca
     1381 aggacatgca ggaggacaag gtcccgacct tcgaggcttt cgacgcgctg gagctgtccc
     1441 tgctggccat ggccggcatg gtcgccgacc tgacgccc
//
LOCUS       DAOMEO010000924         1478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8726_length_1478_cov_2.153197, whole genome shotgun sequence.
ACCESSION   DAOMEO010000924 DAOMEO010000000
VERSION     DAOMEO010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_1478_cov_2.153197"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..930)
                     /locus_tag="PLH31_16935"
     CDS             complement(58..930)
                     /locus_tag="PLH31_16935"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HQR90915.1"
                     /translation="MKPVRKAVLPVAGFGTRVLPGTKTTPKELLNVVDRPILSYVVAE
                     ARAAGIEHFVFVTGRNKGAIEDYFDHQVELEAALAAKGKTDLLNELLDELPKPGEMSF
                     VRQMAPLGLGHAVWCARDIIGDEPFAVLLPDMVMHAQTSALAQAIEAYDQVGGNIVVV
                     EPCPAGQAHQYGIVALDGQEGRLNRMTGMVEKPPKGTEPSNLFISGRYILQPEIFDLL
                     ASQEKGAGNEIQLTDSMLKLMATQEFHALEYEGITYDCGDKIGLLRANVALALAHPEL
                     GAAARSAIEDLLKL"
     gene            1097..>1478
                     /locus_tag="PLH31_16940"
     CDS             1097..>1478
                     /locus_tag="PLH31_16940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR90916.1"
                     /translation="MHRRALLTLMTAGAAGLLAGCGNKKAAENLVAADAFMAKNAKEP
                     GVVTLPEGLQYKVIRSGPNGGMHPTRADEVKVHYEGKLIDGTVFDSSYERGAPASFPL
                     DGLVPAWIIALQRMKAGDEWMLYVP"
BASE COUNT          246 a          494 c          488 g          250 t
ORIGIN      
        1 ccgcattagc cccctcagtc gctccgcgac agctccccca gagggggagc atccaaacta
       61 cagcttcagc agatcctcga tcgccgacct tgccgccgcg cccagttccg gatgcgccag
      121 ggccagggcg acattggccc gcagcaggcc gatcttgtcg ccgcagtcat aggtgatccc
      181 ctcgtattcc agggcgtgga actcctgggt cgccatcagc ttgagcatcg agtccgtcag
      241 ctggatctcg ttgccggcgc ccttctcctg ggacgccagc aggtcgaaga tttccggctg
      301 caggatgtag cggccactga tgaacaggtt ggagggctct gttcccttcg gcggcttctc
      361 gaccatgccg gtcatgcggt tgaggcggcc ctcctggccg tccagcgcca cgatgccgta
      421 ctggtgggcc tgacccgccg ggcagggctc gaccacgacg atgttaccgc cgacctgatc
      481 ataggcttcg atagcctgcg cgagggcgga ggtctgggcg tgcatcacca tgtcgggcag
      541 caacacggcg aagggctcat cgccgatgat gtcacgcgcg caccagacgg catggcccag
      601 acccaggggc gccatctggc gcacgaagct catctcgccg ggcttgggca gttcgtccag
      661 cagctcgttg aggaggtcgg tcttgccctt ggcggccagc gcggcttcca gttcgacctg
      721 atggtcgaaa tagtcctcga tggcgccctt gttgcggccg gtgacaaaga cgaagtgctc
      781 aatgcccgcg gcgcgggctt ccgccacgac ataggacagg atcggccggt ccacgacgtt
      841 caacagttcc ttcggggtcg tcttggtgcc cggcagcacg cgggtgccga agccggcgac
      901 ggggaggact gccttgcgga cgggtttcat gagcgcctca tgggtttcgc ttgcgggagg
      961 gttctagggg ggcaaatcgc gaaacgccac caccgttcga tgaaatcatg gctggcgcga
     1021 gggttccgac gcggcgaaga ttgatcttcg cgcggagcct gctagctgaa ccgccagatc
     1081 agttccggag gcccccatgc atcgccgcgc ccttctcacc ctcatgaccg ccggagcggc
     1141 cggcctgctc gccggctgcg gcaacaagaa agccgccgag aacctcgtgg cggccgacgc
     1201 cttcatggcc aagaacgcca aggagccggg cgtggtcacc ctgcccgagg gcctgcagta
     1261 caaggtcatt cgctcgggcc ccaacggcgg catgcacccg acccgcgccg acgaggtcaa
     1321 ggtccactac gagggcaagc tgatcgacgg cacggtgttc gacagcagct acgagcgcgg
     1381 cgcgcccgcc tcgttcccgc tggacggcct ggtgccggcc tggatcatcg ccctgcagcg
     1441 catgaaggcc ggcgacgagt ggatgctcta tgtgccgc
//
LOCUS       DAOMEO010000925         1476 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8738_length_1476_cov_2.428571, whole genome shotgun sequence.
ACCESSION   DAOMEO010000925 DAOMEO010000000
VERSION     DAOMEO010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8738_length_1476_cov_2.428571"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1307)
                     /locus_tag="PLH31_16945"
     CDS             complement(51..1307)
                     /locus_tag="PLH31_16945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR90917.1"
                     /translation="MGWRRWLGAAVAVGLLASAPSYAAPRSGPSVSLSPGAPETVGFS
                     AERLRRLDGAMQALVEQGHLPGVTTMLVRHGKIVSFQVHGKKGFDGPLMTRDTVFRIY
                     SQTKPVTGVAMMILFEEGKWRLDDPVSKYIPEFANLRVYKGQNPDGSFITEAANRPPT
                     MREVMNHSAGFAYGLVPDNPVDKAYVSTGVLSAKTGQEFIDKVAALPLVGQPGAQWKY
                     SASVDIQGFIVEKLSGMSLADFMRTRIFEPLKMVDTGFYLPAAKADRLASMYVWGPKV
                     QKLVPAEGFMVLDVTKPPVVASGGGGLVSTNADYARFAQMLLNGGELDGARILSPGTI
                     SLMGANHLSDDIMTGKDGSFRNGRGQGFGLDFMVITDPAKAGVLAGEGTISWGGAAGT
                     WFWIDPKNDLFFLGMIHILNKDGDPE"
BASE COUNT          279 a          490 c          454 g          253 t
ORIGIN      
        1 gcggtacaca cggccaagaa atagtcctcg cgcgcgggcg cgaggcacct ctactccgga
       61 tcgccgtcct tgttgaggat gtggatcatg cccaggaaga acaggtcgtt cttcgggtcg
      121 atccagaacc aggtgccggc cgcgccgccc cagctgatcg tgccctcgcc ggccaggacg
      181 ccggccttgg ccggatcggt gatgaccatg aagtccaggc caaagccctg accgcggcca
      241 ttgcggaacg agccatcctt gccggtcatg atgtcatcgg acaggtgatt ggcgcccatc
      301 aggctgatcg tgcccggcga caggatccgc gcgccgtcca gttcgccgcc gttcagaagc
      361 atctgggcga agcgggcata gtcggcattg gtcgacacga ggccgccgcc gcccgaggcg
      421 accaccggcg gcttggtaac gtccagcacc atgaagccct cggccgggac cagcttctgc
      481 accttgggcc cccagacata catcgaggcc aggcgatcgg ccttggcggc tggcaggtaa
      541 aaacccgtat cgaccatctt caacggctcg aagatgcggg tgcgcatgaa gtcggccagc
      601 gacatccccg acagcttctc gacgatgaac ccctggatat cgaccgaggc gctatacttc
      661 cactgcgcgc ccggttggcc caccagcggc agggcggcga ccttgtcgat gaactcctgg
      721 ccagtcttcg cgctcagcac accggtcgag acataggcct tgtcgacggg gttatccgga
      781 accaggccat aggcgaagcc agccgagtgg ttcatcactt cgcgcatggt cggcgggcga
      841 ttggcggcct cggtgatgaa gctgccgtcc gggttttggc ccttgtagac gcgcagattg
      901 gcaaactccg ggatatactt gctgaccgga tcatcgaggc gccacttgcc ctcctcgaac
      961 aggatcatca tcgccacgcc ggtgacgggc ttggtctggc tgtagatccg aaagaccgta
     1021 tcccgcgtca tgagcgggcc atcgaagccc ttcttgccat ggacctggaa gctgacaatc
     1081 ttgccatggc gcaccagcat ggtggtgacc ccgggcaggt gtccctgctc gaccagcgcc
     1141 tgcatggcgc cgtccagtcg cctaagccgc tcagccgaaa agccgaccgt ctcgggcgca
     1201 ccgggagaca ggctgacaga gggaccgcta cggggcgccg catatgaggg cgcggacgcc
     1261 agaagtccga ccgcaaccgc agccccgagc caacgccgcc aacccatctc gccctccaac
     1321 gtatccgaac acttgtttaa gggaccttag ggtcaatcgc atcggttgcg cagctagttt
     1381 ttctgcgtcc tcacgaccat gcgcaggaaa ggccgccatt ggctcgcctc caatgacctg
     1441 ttggccctga ctgcaagaat cctgaggctt ggaccg
//
LOCUS       DAOMEO010000926         1474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8750_length_1474_cov_3.115574, whole genome shotgun sequence.
ACCESSION   DAOMEO010000926 DAOMEO010000000
VERSION     DAOMEO010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_1474_cov_3.115574"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLH31_16950"
     CDS             complement(<1..1037)
                     /locus_tag="PLH31_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90918.1"
                     /translation="MAQVILSSVGAALGGPVGGFVGAMLGRAADSAVINALSPARQLG
                     PRIPELRITGAAEGAAIPCVFGRGRVGGHILWAARFREKRIEGRVGGSKGQKTTSYAY
                     SLSFAVAVGEGPIDGIGRVWADGKVMDMAGVTMRLHRGTQDQTPDPLIEAIEGAAPAY
                     RGLAYVVFEDLPLEAFGNRPPQLSFEVFRRPGSPTGLEARLKGVCLIPGAGEFVYATD
                     AVLRRDGLTRTTAENVNNAEGRPDLVVSLDQLQAQLPEVDHVTLVVSWFGTDLRCGTC
                     QIKPGVDQAFKATLPHEWRAGGVDRAGAHAISTHDGGPAYGGTPSDRSVLQAIAELKR
                     RGLKVTLYPFVL"
     assembly_gap    1109..1118
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1247..>1474)
                     /locus_tag="PLH31_16955"
     CDS             complement(1247..>1474)
                     /locus_tag="PLH31_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HQR90919.1"
                     /translation="GRWLIPVSLAAARPGDVLVFRMEPRAFAKHCAILSAPDRMIHAY
                     WGRACVESWLGRWWRERMVGAFRFPSSSPQN"
BASE COUNT          246 a          523 c          502 g          193 t
ORIGIN      
        1 agcacgaacg gatagagggt caccttcagc ccccgccgct tcagctcggc gatggcctgc
       61 agcaccgagc gatccgacgg ggtgccgcca taggccgggc cgccgtcgtg ggtcgagatc
      121 gcatgggctc cggcgcgatc cacgccgcca gcgcgccact cgtgcggaag ggtcgccttg
      181 aacgcctggt cgacgcccgg cttgatctgg caggtcccgc agcgcaggtc ggtcccgaac
      241 cacgagacca ccagggtcac gtgatccacc tccggcaact gcgcctgaag ctggtccagc
      301 gacacgacga gatcgggccg gccctcggcg ttgttgacgt tctcggccgt ggtccgggtg
      361 aggccgtcgc ggcgcaggac ggcgtcggtg gcgtagacga actcgcccgc gccggggatc
      421 aggcacaccc ccttcagccg cgcctccagc cccgtgggcg agccgggccg gcggaacacc
      481 tcgaacgaca gctgcggcgg gcgattgccg aaggcttcga gcggcaaatc ctcgaacacc
      541 acataggcca gaccgcgata ggccggcgcg gcgccctcga tcgcttcgat cagcgggtcg
      601 ggcgtctggt cctgcgtccc gcgatgcaga cgcatggtca cgcccgccat gtccatcacc
      661 ttgccgtcgg cccagacgcg gccgatcccg tcgatcgggc cttcgcccac cgcgacggcg
      721 aaagacaggc tgtaggcata gctggtggtc ttctgaccct tgctcccgcc cacccggccc
      781 tcgatccgct tctcgcggaa acgggcggcc cagaggatgt ggccacccac ccggccacgc
      841 ccaaacacgc acgggatcgc cgcgccctcg gcggcgccgg tgatacggag ctcgggaatg
      901 cgcggcccca gctggcgggc gggcgaaaga gcgttgatca ccgcgctgtc ggccgcccgg
      961 cccagcatcg cgccgacgaa accgccgacc gggccgccga gggccgcgcc gacggaggag
     1021 aggatcacct gggccatcta gggggctccg ttgtctaggg tggaacggaa ctgagcgttc
     1081 caaaaatcaa cgccttcctg ggccttgtnn nnnnnnnngc ccaggatcca tgcccgcggc
     1141 ttcgctgcct tcaggtcaga ggctagggct tgccgcacgc ggttgagggc ctcaacgcga
     1201 gccgcacgat gggtcctggg cacaaggccc aggaaggcag gcatgttcag ttttggggag
     1261 aggaagacgg aaaccgaaac gccccaacca tccgctccct ccaccaccgc cccagccagc
     1321 tttcgacgca ggcgcggccc cagtaggcgt ggatcatgcg atccggcgcc gacaggatcg
     1381 cgcagtgctt ggcgaaggcc cggggctcca tgcggaacac caggacgtcg ccgggacgag
     1441 cggcggcgag ggaaaccggg atcagccagc ggcc
//
LOCUS       DAOMEO010000927         1473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8757_length_1473_cov_3.047955, whole genome shotgun sequence.
ACCESSION   DAOMEO010000927 DAOMEO010000000
VERSION     DAOMEO010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_1473_cov_3.047955"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLH31_16960"
     CDS             <1..501
                     /locus_tag="PLH31_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518887.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HQR90920.1"
                     /translation="QTTGFDGTVRKADLAPTKVLDRKVTVRFDANIRNLPWEFTTEQT
                     KQDIRIGDTGLAFFKVTNTSNQAMTGRAIYNVVPEQAGPYFQKLECFCFTNQTIQPGQ
                     TIEFPVVYFVDPQYVDDPETKGKGEITLSYTFFPAVEDGPAQKQAVSTTPRIVQPLGG
                     APSRGL"
     gene            574..1449
                     /locus_tag="PLH31_16965"
     CDS             574..1449
                     /locus_tag="PLH31_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQR90921.1"
                     /translation="MAHGAVKHDYHILPPSPWPLVGSVASTIMAIGLIGWLKGGVLGI
                     EKGNWAIFAAGFAGVLYTFFGWWSDVAKEAKAGDHTPVVSIGLRYGMILFIASEVMFF
                     VAWFWMFFEMALFPEARTVSTIEEVRAAWATWPPAGVETVSPWHLPLVNTLTLLLSGT
                     TVTWAHHALQEGDRNGAKWGLILTVLLGALFTAIQFYEYAHINHEQLFYSEAAVNSGL
                     YGSTFFLATGFHGFHVFVGTLFLIVCLIRLQNGAFTPKQHFGFEAAAWYWHFVDVVWL
                     FLFAFIYVIFGASAH"
BASE COUNT          255 a          495 c          435 g          288 t
ORIGIN      
        1 cagacgaccg gcttcgacgg cacggtgcgc aaggccgatc tggccccgac caaggtgctg
       61 gaccgcaagg tcaccgtccg gtttgacgcc aatatccgca atctgccctg ggagttcacg
      121 accgagcaga ccaagcagga catccggatc ggcgatactg gcctggcctt cttcaaggtc
      181 accaacacca gcaatcaggc catgaccggc cgggccatct acaatgtggt gccggaacag
      241 gcggggccgt acttccagaa gctggaatgc ttctgcttca ccaaccagac gatccagccc
      301 ggccagacga tcgagtttcc ggtcgtttat ttcgtcgatc cgcaatatgt cgatgatccg
      361 gagaccaagg gaaagggcga gatcaccctg tcctacacct tcttcccggc ggtcgaggac
      421 ggcccggcgc aaaaacaggc ggtttcaacg acgcctcgta tcgttcaacc ccttggcggc
      481 gcgccgtcga gagggctata gcgttcaata tggagcgttg cggcgggggg actcggtcgg
      541 caacgtttag agggaataag ggttagcacc accatggccc acggcgccgt caaacacgac
      601 tatcacatcc tgccgcccag cccgtggccc ctcgtgggtt cggtcgcctc cacgatcatg
      661 gccatcggcc tgatcggctg gctcaagggc ggcgtcctgg gcatcgaaaa aggcaactgg
      721 gcgatcttcg ccgcgggctt cgccggcgtg ctctacacct tcttcggctg gtggagcgac
      781 gtggccaagg aagccaaggc cggcgaccac accccggtcg tctcgattgg cctgcgctac
      841 ggcatgatcc tgttcatcgc ctcggaagtg atgttcttcg tcgcctggtt ctggatgttc
      901 ttcgagatgg cgctgttccc cgaggcgcgc acggtctcga ccatcgagga agtccgcgcc
      961 gcctgggcca cctggccgcc ggctggtgtc gagaccgtct cgccctggca cctgccgctg
     1021 gtcaacaccc tgaccctgct gctgtcgggc accaccgtga cctgggcgca ccatgccctg
     1081 caggaaggcg accgcaacgg cgccaagtgg ggtctaatcc tgaccgtcct gctgggcgcg
     1141 ctgttcaccg ccatccagtt ctatgaatac gcccacatca atcacgagca gctgttctac
     1201 tcggaagcgg ccgtcaattc gggcctgtat ggctcgacct tcttcctggc caccggcttc
     1261 cacggcttcc acgtgtttgt cggcaccctg ttcctgatcg tctgcctgat ccgcctgcag
     1321 aacggcgcct tcacccccaa gcagcacttc ggcttcgaag ccgccgcctg gtactggcac
     1381 ttcgtcgacg tggtctggct gttcctgttc gccttcatct acgtgatctt cggcgcctca
     1441 gcgcactagg tcatgacctt cgagggggcc cgg
//
LOCUS       DAOMEO010000928         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8767_length_1472_cov_2.143260, whole genome shotgun sequence.
ACCESSION   DAOMEO010000928 DAOMEO010000000
VERSION     DAOMEO010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8767_length_1472_cov_2.143260"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLH31_16970"
     CDS             <1..1009
                     /locus_tag="PLH31_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQR90922.1"
                     /translation="LQYSVSEGYLPLREWIAERMTRDGMPCDAGNIMLTAGSQQALDL
                     LGKLFLTRGDTVMVARPTYLGALQAFNGYEPTYLDMPETGLADGLDAATLDRAKGCLG
                     YFVPDFANPTGQSLTLDEREALLDLAGDLDMTLIEDAAYRELRFSGEALPTLLSLDIA
                     RSGGIEAARTLFCGTFSKTLSPALRIGWVCGPRAVIEKLVLLKQGADLHVSTINQMVA
                     HRAVIEGFDQHLGMLRGAYGANARVMLAALERHMPAGVTWSKPHGGMFVWVWLPEGLD
                     GKVVLERALAEERVAFVPGEPFFAEVPTANALRLSYSLPTDAQIEDGVARLARLIGRM
                     G"
     gene            complement(1133..>1472)
                     /locus_tag="PLH31_16975"
     CDS             complement(1133..>1472)
                     /locus_tag="PLH31_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQR90923.1"
                     /translation="KDAQLACLRDALDELEYEGGRPRTIGSDWARGDLASVRARYSTS
                     AAQRCLLRSPGGAKLLEQQISQSANALSSALNRPGVTVAVVDLAFLLPANGVLDRLKA
                     GGATVTSPPD"
BASE COUNT          228 a          499 c          507 g          238 t
ORIGIN      
        1 gctgcaatat tcggtcagcg agggctatct gccgctgcgc gaatggatcg ccgagcgcat
       61 gacccgcgac ggcatgccgt gcgacgcggg caacatcatg ctgaccgccg gctcgcagca
      121 ggcgttggac ctgctgggca agctgttcct gacgcgcggc gacacggtga tggtggcccg
      181 gcccacctat ctgggcgcgc tgcaggcctt caacggctac gagccgacct atctggacat
      241 gccggaaacc ggcctggccg acggcctgga cgccgcgacg ctggatcggg caaagggttg
      301 cctgggctat ttcgtccccg acttcgccaa tcccaccggc cagagcctga ccctggacga
      361 gcgggaggcc ctgctggacc tcgccggcga cctggacatg accctgatcg aggacgccgc
      421 ctatcgcgag ctgcgcttca gcggcgaggc cctgccaacc ctgctgtcgc tggacatcgc
      481 ccggtcaggc gggatcgagg cggcccgcac cctgttttgc ggcaccttct ccaagaccct
      541 ctcgccggcc ctgcgcatcg gctgggtctg cggtccgcgc gcggtgatcg agaagctggt
      601 tttgctgaaa cagggcgccg acctgcacgt ctcgaccatc aaccagatgg tcgcccaccg
      661 cgccgtgatc gagggctttg accagcacct gggcatgctg cgcggcgcct atggcgccaa
      721 cgcgcgggtg atgctggcgg ctcttgagcg ccacatgccg gcgggggtca cctggtcgaa
      781 gccccacggc ggcatgttcg tctgggtctg gctgcccgag ggcctggacg gcaaggtcgt
      841 gctcgaacga gcgctcgccg aggaacgtgt cgcctttgtg ccgggcgagc cgtttttcgc
      901 ggaggttccg accgccaacg ccctgcggct gagctattcg ctgccgaccg acgcgcagat
      961 cgaggatggc gtggcgcggc tggcgcggtt gatcgggcgg atggggtaga gccgtgcgtc
     1021 tttcgagacc ctcctacgga gggcttctca ggatgaggaa gtcaacgaaa gcgtgctaat
     1081 cctgaggtcc gagccgaagg tgagcctcga aggacgcaag gctggtccca cgtcaatccg
     1141 gcggcgacgt caccgttgcg ccgccagcct tcagcctgtc cagcacgcca ttggccggca
     1201 gaaggaacgc cagatcgacc accgccaccg tgacgccggg ccggttcagc gcgctcgaca
     1261 acgcattggc cgactggctg atctgctgct ccagaagttt ggcgccgccg ggcgaccgga
     1321 gcaggcagcg ctgggcggcg ctggtcgaat agcgggcgcg gacgctggcc agatcgcccc
     1381 gcgcccagtc cgaaccgatg gtcctgggcc ggccgccttc atattccagc tcgtccaggg
     1441 cgtcgcgcaa acaggccagc tgggcgtcct tc
//
LOCUS       DAOMEO010000929         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8768_length_1472_cov_2.119266, whole genome shotgun sequence.
ACCESSION   DAOMEO010000929 DAOMEO010000000
VERSION     DAOMEO010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_1472_cov_2.119266"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLH31_16980"
     CDS             complement(<1..274)
                     /locus_tag="PLH31_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HQR90924.1"
                     /translation="MPLLMCPNCDGSMQAVNRAGVEFDMCPKCRGVWLDRGELEKLMA
                     LEREDAAGQAAPPPRQQTQYQAPPQQNYAPPPRYEEPRRYKDDDVRH"
     gene            complement(747..1364)
                     /gene="rpsD"
                     /locus_tag="PLH31_16985"
     CDS             complement(747..1364)
                     /gene="rpsD"
                     /locus_tag="PLH31_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQR90925.1"
                     /translation="MSKRHSAKYKIDRRMGENLWGRPKSPVNQRSYGPGQHGQRRKQK
                     VSDFGLQLRAKQKLKGYYGNLTEKQFSRTYEEAARRKGNTSENLISLLESRLDAIVYR
                     AKFVPTVFAARQFVNHGHVTVNGKRVNIASYRCKAGDVIAVRERSRNMALVLEAVASA
                     ERDFAEYITVDVKGLSATFVRAPELSEVPYPVKMEPNLVVEFYAS"
BASE COUNT          255 a          439 c          502 g          276 t
ORIGIN      
        1 gatgacgaac atcgtcatcc ttgtagcggc gcggctcttc atagcggggc ggcggcgcat
       61 agttttgctg cggaggggcc tggtactggg tctgctgacg cggcggcggc gcggcctgac
      121 ccgcggcgtc ttcccgctcc agggccatca gcttttccag ttcgccgcga tccagccaga
      181 caccccggca cttggggcac atgtcaaact caacgccggc gcggttcacg gcctgcatcg
      241 aaccatcgca gttggggcac atcagaagcg gcatatggcg tttccctatc gagactggag
      301 gggtgctctc gacgctgagt ccggaccgag ggagggaggg ggggacaacc ggacccagac
      361 atcgagaaca cccgacgcgg tccgacatca cgaatgcttg cattcactcg tcagaggggc
      421 ccggcccgcg cattgaaact atgcagcgcg acgaggcgct tcaaggggga ccctgaaaaa
      481 agatcctctt cagggggtga agcagggctt tcgggttcgc agctaaaatc tgtcatcccg
      541 gacaaggccc gtagggccgc cgatccggga ccgccggaag cgccgagttc aggcgtcggt
      601 cccggatctt cgcgctgcgc gctcgtccgg gatgacaacc gcttggtcat gagcgatagc
      661 gctgccctga gaagggcgtc tgccgaacga aaaaggcccc ggatctctcc ggggcctttc
      721 cgataggtct tcgaggtcga ggcttattac gaagcgtaga attcgacgac gaggttgggt
      781 tccatcttca ccgggtacgg cacttccgac agttccggag cgcggacgaa ggtggccgac
      841 aggcccttga cgtcgacggt gatgtattcg gcgaagtcgc gttcggccga ggcgacggct
      901 tccagcacca gggccatgtt gcgcgagcgc tcacggacgg cgatgacgtc gccggccttg
      961 cagcggtagg aggcgatgtt gacgcgcttg ccgttcacgg tcacgtggcc gtggttcacg
     1021 aactggcggg cggcgaacac ggtcggcacg aacttggcgc gatagacgat ggcgtccagg
     1081 cgcgactcga gcagcgagat gaggttttcc gaggtgttgc ccttgcggcg ggcggcttcc
     1141 tcgtaggtgc gcgagaattg cttctcggtc aggttgccgt agtagccctt cagcttttgc
     1201 ttggcgcgca gctgcagacc gaagtcggaa accttctgct tgcggcgctg gccgtgctgg
     1261 ccggggccgt aggaccgctg gttgaccggc gacttgggcc ggccccagag gttttcgccc
     1321 atacggcgat cgatcttgta cttggcgcta tggcgcttgg acatgtgtag ctcttcagtt
     1381 cgatccgggc cggcgcctca agaagtagag gcgcgatctc aacggcggcg tcgcatcagt
     1441 ccgtcatata tttccacgcc gcacggcgag cc
//
LOCUS       DAOMEO010000930         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8769_length_1472_cov_2.098800, whole genome shotgun sequence.
ACCESSION   DAOMEO010000930 DAOMEO010000000
VERSION     DAOMEO010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8769_length_1472_cov_2.098800"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLH31_16990"
     CDS             complement(<1..382)
                     /locus_tag="PLH31_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90926.1"
                     /translation="MLRRSALIALAMVVVATPAMAEKIAPAAKAFPYLEKFLKVPAAE
                     RSRLRLGYVLTREGKPLSNLKVALIEANGTRTPLPVDGSGGFERLPTLAQLEAGARLS
                     LDLPQDAKVGTMMNFSTQLKPAVDY"
     gene            complement(391..1278)
                     /locus_tag="PLH31_16995"
     CDS             complement(391..1278)
                     /locus_tag="PLH31_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR90927.1"
                     /translation="MMLPDISWSRRGLLVAAAATGLLGCGQKAADDAPKPPAKAGVDT
                     IEAAVNGDWRSSADRARDMWRHPIESLKFWELKPGQTVVEFWPGAGWYTEILAPFLAA
                     TGGKLYAAGLQSDPGDLAAVEVVEAYRRKLTEKSRIYGAVDITAFGPTSGPVAPAGSA
                     DLVLFLRNIHNWMAAGIAEKAFRDALAALKPGGVLGVEEHRGEAGRVQDVLAADGYVQ
                     EDYVVQLAREAGFIVAGASEINANPKDSKDHPFGVWTLPPTRLSSPRGDPAEPGFDHS
                     KYDAIGESDRMTLKFIKPK"
     gene            complement(1334..>1472)
                     /locus_tag="PLH31_17000"
     CDS             complement(1334..>1472)
                     /locus_tag="PLH31_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR90928.1"
                     /translation="FGVWTLPPVKRTAPGGQPADPTFDRSKYDAIGESDRMTLRFRKP
                     S"
BASE COUNT          247 a          495 c          484 g          246 t
ORIGIN      
        1 cgtagtcgac agctggcttc agctgggtcg agaagttcat catcgtgccg accttggcgt
       61 cctggggcag gtccagcgac agcctggccc ccgcctccag ctgcgccagg gtcggcaggc
      121 gctcgaaacc accggagcca tcgaccggca agggcgtacg agttccgttg gcctcgatca
      181 gcgccacttt gaggttcgac agcggcttgc cctcgcgggt cagtacatag cccagccgca
      241 gcctggagcg ctcggcggcc ggcaccttca ggaacttttc gagataggga aaggccttgg
      301 cggccggggc gatcttctcg gccatggccg gcgtggcgac gacgaccatc gccagggcga
      361 tcagagcgga acggcggagc atcgaagatc tcatttgggc ttgatgaact tgagcgtcat
      421 gcgatcgctc tcgccgatgg cgtcgtactt gctgtggtca aagcccggtt ctgcgggatc
      481 gccgcgaggc gacgacaggc gcgtgggcgg caaggtccag acgccaaacg gatggtcctt
      541 gctgtccttg ggattggcgt tgatctcgct ggcgccggcc acgatgaagc ccgcctcgcg
      601 cgccagctgc acgacatagt cctcctggac gtagccatcg gccgccaaca cgtcctgcac
      661 ccgccctgct tcgccgcggt gctcttcgac gcccagcacg ccgcccggtt tcagggcggc
      721 aagagcgtca cggaacgcct tttcggcgat gcccgccgcc atccagttat ggatgttgcg
      781 caggaaaagc acgaggtcag ccgagccagc gggcgcgacg ggaccgctgg tcggaccaaa
      841 agcggtgatg tcgaccgcgc catagatcct ggacttctcg gtcagcttgc ggcgataggc
      901 ctcgacgacc tcaaccgcgg ccagatcgcc ggggtcgctc tgcagtccgg cggcatagag
      961 cttgccgcca gtggccgcga ggaacggcgc caggatctcg gtgtaccagc cggcacccgg
     1021 ccagaattcc acgacggtct gccccggctt gagctcccag aacttcaggc tttcgatcgg
     1081 gtggcgccac atgtcccgcg cgcggtcagc ggacgatcgc cagtcgccgt tgaccgccgc
     1141 ctcgatcgtg tcgacgccgg ccttcgccgg cggcttgggc gcatcgtcgg ccgccttctg
     1201 cccgcaacct aagagacctg tcgccgctgc ggcgacgagc aggcctcgcc tagaccagga
     1261 aatatccgga agcatcatcc ccccttacga acgccgccag actatttcgc gaccttggcc
     1321 aaaaggcgcc ggactacgat ggcttgcgga aacgcagggt catccggtcg ctttcgccga
     1381 tcgcgtcata cttgctgcga tcaaaggtag gatccgcagg ctggccgccc ggcgcggtgc
     1441 gcttgacggg cggcagggtc cagacgccga ag
//
LOCUS       DAOMEO010000931         1472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8773_length_1472_cov_1.584333, whole genome shotgun sequence.
ACCESSION   DAOMEO010000931 DAOMEO010000000
VERSION     DAOMEO010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_1472_cov_1.584333"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="PLH31_17005"
     CDS             complement(<1..79)
                     /locus_tag="PLH31_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90929.1"
                     /translation="MSRQPPIRIGARGSKLSLAQSGLMQA"
     gene            152..1249
                     /gene="tsaD"
                     /locus_tag="PLH31_17010"
     CDS             152..1249
                     /gene="tsaD"
                     /locus_tag="PLH31_17010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQR90930.1"
                     /translation="MQTLKTLTILGLETSCDETAASVVRRAGDGTVTVLSSVIGTQFE
                     QHAPFGGVVPEIAARAHVEAIDAIAAEAMRAAGVGFDDINGVAATAGPGLVGGVMVGL
                     AFGKAVALARDVPLVAVNHLEGHAVSARLGADIPYPFLLLLVSGGHCQLLEVAGVGAC
                     TRLGTTIDDAAGEAFDKIAKSLGLPYPGGPALEKLATTGDASKFDLPRALLGRKDCDF
                     SFSGLKTAAARQAEKITDQQSRADLAAAVQATIARQLSERTDRAMKLYKDSHPGQPLR
                     FVVAGGVAANVAVKAALKKNCDDNGFSFDAPPLAYCTDNAAMIALAGAERLALGISDD
                     LDAVARPRWPLDEAAALSNPSNSFGRKGAKA"
     gene            1246..>1472
                     /locus_tag="PLH31_17015"
     CDS             1246..>1472
                     /locus_tag="PLH31_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQR90931.1"
                     /translation="MTFTHAGVIGAGAWGTALAQVCARAGLKVTLQAREAEVVESIAA
                     QRENSLFLPGVVLEPEIAAVADMAGLADCDL"
BASE COUNT          227 a          501 c          515 g          229 t
ORIGIN      
        1 gggcctgcat gaggcccgat tgcgccaggg acagcttgga gccacgggcg ccgatacgga
       61 tgggaggttg ccgggacacg gtctgaacgt tacctgcggg gacgagattt gaagccctgc
      121 tgctacagga ccggcggaaa gcccgcaacc gatgcaaacc cttaagaccc tgacgatcct
      181 tggcctggag accagctgcg atgagaccgc ggcctcggtc gtgcgccgcg cgggtgacgg
      241 aacggtcacg gtgctgtcct cggtcatcgg cacgcagttc gagcagcacg cgccgtttgg
      301 cggggtcgtg ccggaaatcg ccgcccgcgc ccatgtcgaa gccatagacg ccatcgcggc
      361 cgaggccatg cgggcagcgg gggtcgggtt cgatgacatc aacggcgtcg ccgctacggc
      421 cggtcccggc ttggtcggcg gggtaatggt cggcctggcg ttcggcaagg ctgtggccct
      481 ggcgcgtgat gtgccgctgg tggcggtcaa tcacctggaa ggtcatgcgg tctcggcccg
      541 gctgggcgcc gacattccct atcccttcct gctgctgctg gtctcgggcg gccactgcca
      601 gctgctggag gtggccgggg tcggggcctg cacgcgcctg ggcaccacca tcgacgatgc
      661 ggccggcgag gccttcgaca agatcgccaa gtccctgggc ctgccctatc cgggcggtcc
      721 ggcgctggaa aagctggcga ccacgggcga cgcctccaag ttcgacctgc cccgcgccct
      781 gctgggccgc aaggactgtg acttctcgtt ctcgggcctg aagaccgccg ccgcgcgcca
      841 ggccgaaaag atcactgacc agcagtctcg cgccgatctg gccgccgccg tccaggccac
      901 catcgcccgt cagctgtccg aacgcaccga ccgggcgatg aagctgtaca aggacagtca
      961 tccaggccag ccgctgcgct tcgttgtcgc cggcggtgtc gccgccaatg tcgcggtcaa
     1021 ggcggcgctg aagaagaact gcgacgacaa cggcttttcg ttcgacgccc cgcccctggc
     1081 ctattgcacc gacaacgccg cgatgatcgc cctggccggg gccgagcggc tggctttggg
     1141 catcagcgac gatctcgacg ctgtcgcccg tccgcgttgg cctttggacg aggcggcggc
     1201 gttatccaat ccgagcaaca gtttcggtcg caagggagcc aaggcatgac cttcacgcac
     1261 gcaggcgtca tcggcgccgg agcctggggc acggccctgg cccaggtctg cgcccgggcg
     1321 ggcctgaagg tcacgctgca ggcccgcgag gccgaggtgg tcgaaagcat cgccgcccag
     1381 cgggaaaaca gcctgttcct gcccggcgtc gtgctggagc ccgagatcgc cgccgtcgcc
     1441 gacatggccg gcctggcgga ctgcgacctg at
//
LOCUS       DAOMEO010000932         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8784_length_1470_cov_3.162544, whole genome shotgun sequence.
ACCESSION   DAOMEO010000932 DAOMEO010000000
VERSION     DAOMEO010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8784_length_1470_cov_3.162544"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>277
                     /locus_tag="PLH31_17020"
     CDS             <1..>277
                     /locus_tag="PLH31_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HQR90932.1"
                     /translation="SAKIFHVSPFMPMDLRYVFEIAPPGPAVHVGIDVFDAEGLALAA
                     NFIGRRVEMTDRALTHAVLSHPLQMLGVLVGIHWEALKMALKGFGLFG"
     assembly_gap    278..287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(392..>1470)
                     /locus_tag="PLH31_17025"
     CDS             complement(392..>1470)
                     /locus_tag="PLH31_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR90933.1"
                     /translation="ELRGNIGTRYAITDVQSDGLTNTARPISGENKYTDLLPSLNAAY
                     EVADNMILRFGAAKVMARPLLGNLAPNVTSISVPTNGNTVGGSLTVGNPKLDPFRAVN
                     LDLSLEWYFAPGALISVAVFDKDISSFPQTLINSTSLSAFLDADGVAALRAAQTNVNA
                     IAYIDANNPFDVRQFGNAPGGYIRGIEFNYQQDLTFLPWHFKNLGVQFNATHLESELE
                     YIVTPANARLGKAAITAKGPFTGASPDAFNFTVYYEVEKFTARVSSAYRTEYRTQYPI
                     ATGTCEPGFCDSPLVNDFLGNDSTFNMDASATYKFSKNITLTLEALNLTDQTENRWAY
                     QDDPVVTRYAAVGRSFFAGIRFVY"
BASE COUNT          255 a          408 c          512 g          285 t
ORIGIN      
        1 agcgccaaga tctttcacgt ctcgccgttc atgcccatgg acctgcgcta tgtgttcgag
       61 atcgcgccgc ccggcccggc ggtgcatgtg gggatcgacg tctttgacgc cgaaggcctg
      121 gcgctggcgg ccaatttcat cggacgacgg gtcgagatga ccgacagggc cctgacccac
      181 gccgtattga gccatccgct gcagatgctg ggtgttctgg tgggcattca ttgggaggcg
      241 ctgaagatgg cgctgaaggg ttttggcctg ttcggctnnn nnnnnnngtt cggctcgccc
      301 tcgccctcgg gggtcttgcc cttgccgcaa gaaaaaggcg gcgctggata agccagcgcc
      361 gccagtcgtc tagatcgggg aggaagaccg tctagtagac gaaccggata cccgcgaaga
      421 acgagcgacc caccgccgca tagcgggtca cgaccgggtc atcctgatag gcccagcggt
      481 tctcggtctg gtcggtgagg ttcagggcct caagcgtcag cgtgatgttc ttgctgaact
      541 tgtaggtcgc cgaggcgtcc atgttgaaag tgctgtcatt gcccaggaag tcgttgacca
      601 gcggcgagtc gcagaagccg ggttcgcagg tgcccgtggc gatcggatac tgggtccgat
      661 actcggtgcg ataggcgctc gacacgcggg cggtgaactt ctcgacctcg taatagacgg
      721 tgaagttgaa ggcgtccggc gaggcacccg tgaaaggacc cttggcggtg atcgcggcct
      781 tgcccaagcg ggcattggcc ggagtgacga tgtattcgag ttccgattca aggtgcgtgg
      841 cgttgaactg aacgcccagg ttcttgaagt gccagggcag gaaggtcagg tcctgctgat
      901 agttgaactc gatgccgcgg atgtagccgc ccggcgcatt gccgaactgg cgaacgtcga
      961 acggattgtt ggcgtcgata taggcgatcg cattgacatt ggtctgggcc gcacgaaggg
     1021 ccgcgacacc gtcggcgtcc aggaacgcgc tcaggctggt cgaattgatc agggtttgcg
     1081 ggaagctgga gatgtccttg tcgaacacgg ccaccgagat cagggcgccg ggggcgaagt
     1141 accactccag gctcaggtcc aggttcaccg cgcggaacgg atccagcttc gggttgccga
     1201 ccgtcagcga gccgccaacg gtgttgccgt tggtgggaac actgatcgac gtcacgttcg
     1261 gcgccaggtt gcccagcagc ggacgggcca tgaccttggc ggcgccgaag cgcaggatca
     1321 tgttgtcggc gacttcatag gcggcgttca gcgagggcag caggtcggtg tacttgttct
     1381 cgcccgagat cggccgtgcg gtgttggtca ggccgtccga ctgcacgtcg gtgatggcgt
     1441 agcgggtgcc gatattgccg cgcagttcgc
//
LOCUS       DAOMEO010000933         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8809_length_1466_cov_2.447201, whole genome shotgun sequence.
ACCESSION   DAOMEO010000933 DAOMEO010000000
VERSION     DAOMEO010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_1466_cov_2.447201"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLH31_17030"
     CDS             complement(<1..1218)
                     /locus_tag="PLH31_17030"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR90934.1"
                     /translation="MRNPALSLGGDRPTSRHVRQNANLLSDLLAEAIAYLEGDDAAAL
                     VTKARKASARDDESDAAPVLDHLFADLSNEQAVFLARAFASHSLLANIGEDVAGRRRH
                     AEAAALPGDERPRTLVDAVAALKAGGKSDADIARIFAAMNVVPVLTAHPAEVRRRSMV
                     DRETEISRLMALRRHHLPADLESDIRERLFREIALMWRTRLYRPERITVKDEIRNALS
                     IVRTSILPAIIDLYGDWTAQIAHNAELAPLLKMGSWLGGDRDGHPGVNGDTLKLALSS
                     QSRVILDWYAGEVRKLWSNLAISTAYTPVSDELMALAGQAKDPSVHRIDEPYRRALEL
                     VFDRLTAVSQKLTGQPVAYANGATDVEPYAHPDGFVADLSIVIDSLARNGGERLVGTS
                     LRTLVEVAKACGFH"
     gene            complement(1385..>1466)
                     /locus_tag="PLH31_17035"
     CDS             complement(1385..>1466)
                     /locus_tag="PLH31_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-deoxy-D-gluconate 3-dehydrogenase"
                     /protein_id="HQR90935.1"
                     /translation="AVFLASPAADYVQGITLPVDGGWLAR"
BASE COUNT          254 a          480 c          505 g          227 t
ORIGIN      
        1 gtggaagcca caggccttgg ccacctcgac cagggtccgc agggacgtac ccaccagacg
       61 ctcgccgcca ttgcgcgcca gactgtcgat gacgatcgac aggtcggcca caaaaccgtc
      121 gggatgggca tagggctcca cgtccgtcgc gccattggca taggcgacag gctggcccgt
      181 cagcttctgc gacacggcgg tgaggcggtc aaagaccagc tccagggccc ggcgataggg
      241 ctcgtcgatg cgatgcaccg acggatcctt ggcctggccg gccagggcca tcagctcgtc
      301 cgataccggc gtataggccg tcgagatcgc caggttcgac cacagcttgc ggacctcgcc
      361 ggcgtaccag tcgaggatca cccgtgactg cgaactcagg gccaacttca gggtgtcgcc
      421 attgacgccg ggatggccgt cgcggtcgcc gcccagccac gagcccatct tcagcagtgg
      481 cgccagctcg gcattgtgag caatctgggc ggtccagtcg ccgtataggt caatgatggc
      541 cggcaggatc gatgtgcgga cgatcgacag ggcgttgcgg atctcgtcct tcacggtgat
      601 ccgctcggga cgatagagcc gcgtgcgcca catcagggcg atttcgcgaa acagccgctc
      661 gcggatgtcg ctctccagat cagccggcag atggtggcgg cgcagggcca tcagccgcga
      721 gatctcggtc tcgcggtcca ccatcgagcg gcggcgcacc tcggccgggt gagcggtcag
      781 gacgggcacc acattcatgg cggcgaagat cctggcgatg tcggcgtcgg acttgccgcc
      841 agccttcagg gcggcgacgg cgtcgaccag ggtgcgggga cgctcgtcgc ccggcagggc
      901 ggcggcctcg gcgtggcgac gacggccggc gacgtcttcg ccgatattgg ccaacagcga
      961 atggctggca aaggcccggg ccaggaacac ggcctgctcg ttggacagat cggcgaacag
     1021 gtgatccaga accggcgcgg cgtcgctctc atcgtcgcgg gccgaggcct tgcgggcctt
     1081 ggtgaccagg gcggcggcgt cgtcgccttc cagataggcg atggcctcgg ccagcaggtc
     1141 gctcagcaga ttggcgttct ggcggacgtg gcggctggtg gggcgatcac cgccgagcga
     1201 cagggccggg ttacgcatga gcgagctccg ctgacgccag aggcgcctct acaaaggatc
     1261 aacttgaacg ccctgcccca tccgagactg acgtaacggc gcccttagcg cgcttcgccg
     1321 tccgggaaaa cccggacggc atgatggttg cgctaccatc ccatctttgc gtgaatctat
     1381 gctgttagcg ggccagccaa ccgccatcca cgggcagagt aatcccctgg acatagtccg
     1441 ctgccggcga cgccaggaac acggcc
//
LOCUS       DAOMEO010000934         1465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8812_length_1465_cov_2.365248, whole genome shotgun sequence.
ACCESSION   DAOMEO010000934 DAOMEO010000000
VERSION     DAOMEO010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8812_length_1465_cov_2.365248"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLH31_17040"
     CDS             complement(<1..844)
                     /locus_tag="PLH31_17040"
                     /inference="COORDINATES: protein motif:HMM:NF016693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR90936.1"
                     /translation="MTVPPLGRVAIIGGGVEAWMTAAGLARATGGQARIRVVETGPAA
                     TGALSTLPSLRAFHALLGLDETALMAATGATYKLGSRFSGWTPGLSFCDAFGEIGANL
                     EGVGFHHYWTRLRQAGDVTPLDDYSLAAVIARLGRFSPPDPDPRSPLSTLAYGLHLDA
                     ALYVGVLRGLALKGGVAASAGEVAQVIRDSETGNITAVALTDGERVEAELFIDCTGPA
                     ARLIGDEPFEDWSALLPADRAVRITAEAPRDAPPLTEIEAVADGWRWRIPLRDRLDSG
                     LTYRA"
     gene            complement(841..>1140)
                     /locus_tag="PLH31_17045"
     CDS             complement(841..>1140)
                     /locus_tag="PLH31_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR90937.1"
                     /translation="RDKAAAPIPDSLAYKMELFRDRGMVVKYRDGFFLEPSWLAVYLG
                     QNILPRGYDPMADSIDPDEAARKLAELKAAVRRTAEAMPGHQAFIERFCGARGAA"
     assembly_gap    1141..1150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1151..>1465)
                     /locus_tag="PLH31_17050"
     CDS             complement(<1151..>1465)
                     /locus_tag="PLH31_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR90938.1"
                     /translation="ISDDEAARVLVSKLDGKAQADPRFLRFTTGRRKKQWNRNVVAIG
                     LASGFLEPLESTSIHLIQVAITTLLELFPERGCAGADQDEYNRVMDLEFERIRDFLVL
                     HYH"
BASE COUNT          218 a          505 c          528 g          204 t
ORIGIN      
        1 ccgcgcgata ggtcaggccg ctgtccagcc ggtcgcgcag cgggatccgc cagcgccagc
       61 cgtcggcgac cgcttcgatc tcggtgaggg gcggggcgtc gcgcggggcc tcggcggtga
      121 tccgcaccgc gcgatcggcg ggcagcaggg ccgaccagtc ctcgaacggc tcatcgccga
      181 tcaaccgggc ggcggggccg gtgcagtcga taaagagctc ggcctcgacc cgctcgccgt
      241 cggtcagcgc gacggcggtg atgtttccgg tctcgctgtc gcggatcacc tgggcgacct
      301 cgccggcgct ggccgcgacg ccgcctttca gggccaggcc gcgcagcacg cccacataga
      361 gcgccgcgtc caggtgcagg ccataggcca gggtcgagag cggcgagcgc ggatcgggat
      421 ccggcggcga gaagcggccc agccgcgcga tcacggcggc caggctatag tcgtccagag
      481 gcgtgacgtc gccagcctga cgcaggcggg tccagtagtg gtgaaagccc acgccctcca
      541 gattggcccc gatctcgccg aaggcgtcgc agaagctcag tcccggcgtc cagccgctga
      601 accgcgagcc cagcttgtag gtggccccgg tggcggccat cagcgccgtc tcgtccaggc
      661 ccagcagggc gtggaaggcg cgcagcgagg gcagggtgga tagggcgcca gtcgcggccg
      721 gcccggtctc gacgacacgg atcctggcct gaccgccggt ggcgcgggcc aggcccgcgg
      781 ccgtcatcca ggcttcgacg ccgccgccga tgatcgcgac gcggccaagg ggaggaacgg
      841 tcatgcagcg ccccgcgcgc cgcagaaccg ttcgataaac gcctgatgcc ccggcatggc
      901 ctcagccgtg cggcgcaccg ccgccttcag ctcggccagc ttgcgggcgg cctcgtccgg
      961 atcgatcgag tctgccatcg gatcatagcc gcgcggcagg atgttctgcc ccagatagac
     1021 ggccagccac gagggctcca ggaagaagcc gtcgcgatac ttcaccacca tgccccggtc
     1081 gcggaacagc tccatcttgt aggccaggct gtcggggatg ggcgcggcgg ccttgtcgcg
     1141 nnnnnnnnnn gtgatagtgc agcaccagga agtcgcggat ccgctcgaac tccaggtcca
     1201 tcacgcggtt gtactcgtcc tggtcggcgc cggcgcagcc gcgctcgggg aacagttcca
     1261 gcagggtggt gatggccacc tggatcaggt ggatgctggt gctttccagc ggctccagga
     1321 agccggacgc caggccgatg gccacgacat tgcggttcca ctgcttcttg cgacgcccgg
     1381 tcgtgaaccg caggaagcgc gggtcggcct gggccttgcc gtccagcttg gagaccagca
     1441 cccgggcggc ttcgtcgtcg ctgat
//
LOCUS       DAOMEO010000935         1461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8844_length_1461_cov_1.876956, whole genome shotgun sequence.
ACCESSION   DAOMEO010000935 DAOMEO010000000
VERSION     DAOMEO010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8844_length_1461_cov_1.876956"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLH31_17055"
     CDS             complement(<1..165)
                     /locus_tag="PLH31_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90939.1"
                     /translation="MAAIAAHKFQIFRMLVETAPDSALRSLELDLASVGCVGGLAQVR
                     GLVEDETTDRF"
     gene            complement(353..>1461)
                     /locus_tag="PLH31_17060"
     CDS             complement(353..>1461)
                     /locus_tag="PLH31_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR90940.1"
                     /translation="LKVIERSRSLFDSDLMVSSEQVDRLVAAPGKTLFFEMRGPQDKP
                     LRAAAILTQLPGHTNPVVFIAAEDRSPIDADAARFGRITALALLLLGGGLILAVVVQV
                     RVGLAPLFQLRREVASVRRGKAERLVRVYPEELEPLAVELNALLAHNQEVVERQRTHV
                     GNLAHALKTPLSVMLTEASQQPGSLAEVVTRQADVMREQVDHHLRRARAAARSQTSGE
                     RTPVEPILDELAVTLERIFQDKSDGRGIEIDWRCPEDLCFQGERQDLMELAGNVMENA
                     GKWCRSKIRAVASPTTAERWTLVVDDDGPGLPPERWVDALKRGQRMDEHTPGSGLGLS
                     IVDELARAYGGSVTLGESPLGGLRVCLDLPRSET"
BASE COUNT          247 a          495 c          500 g          219 t
ORIGIN      
        1 gaagcggtca gtggtctcgt cctcgacaag gccgcgcacc tgcgccagtc cgcctacgca
       61 gccgacgctg gccaggtcca gttccagact gcgcagggca gagtcgggcg cggtttcaac
      121 caacatccga aagatctgaa atttgtgcgc agcgattgcg gccatgatcc cccgccggat
      181 cgaaaagacc gacagcgtca accatgttgg caacgaggtt aagactttcc caaacctgtc
      241 aaaaaagctg gtgggttcct gggctcaggc ggcgatggaa agatcatagg cgccagaggt
      301 ttagcgatgg gcgcgaggcg ggcggcgaca caacctgcgc gacaagcggt cgctaggttt
      361 cagaccttgg caggtcgagg caaacgcgca ggccgcccag gggcgattca ccaagcgtga
      421 ccgacccgcc ataggcgcgg gccagttcgt cgacgatgga aagacccagg cccgagccgg
      481 gcgtatgctc gtccatgcgc tgtccgcgct tgagggcgtc gacccagcgc tcgggcggca
      541 ggcccgggcc gtcgtcgtcg accaccagcg tccagcgctc ggccgtggtg ggcgaagcca
      601 ccgcgcggat cttgctccgg caccacttgc cggcgttttc catcacgttg ccggccagct
      661 ccatcaggtc ctgccgctcg ccctggaagc acaggtcttc aggacagcgc cagtcgatct
      721 cgatgccccg cccgtccgac ttgtcctgga agatccgctc cagggtcacg gccagctcgt
      781 ccaggatcgg ctccaccggc gtgcgctcgc cgctggtctg cgagcgggcg gcggcgcggg
      841 cgcggcgcag gtggtggtcg acctgctcgc gcatcacgtc ggcctggcgg gtcaccacct
      901 cggccaggct gcccggctgc tggctggcct cggtcagcat caccgacagc ggggtcttca
      961 gggcgtgggc cagattgccg acatgggtgc gctgacgctc caccacttcc tggttgtggg
     1021 ccagcagggc gttcagctcg acggccagcg gctccagctc ctcgggatag acccgcacca
     1081 gccgttcggc cttgccgcgg cgcaccgaag cgacttcgcg gcgcagctgg aacagaggcg
     1141 ccaggcccac ccgcacctgc acaaccacgg ccaggatcag gccgccgccc agcagcagca
     1201 gggccagggc ggtgatacgg ccaaaccgcg cggcgtcggc gtcgatgggc gagcggtctt
     1261 cggcggcgat gaacaccaca ggattggtgt ggcccggcag ctgggtgagg attgcggcgg
     1321 cgcgcagcgg cttgtcctgc ggaccgcgca tttcgaagaa cagggtcttg cccggggcgg
     1381 ccaccagccg gtcgacctgc tcggacgaga ccatcaggtc gctatcgaac agcgaacgcg
     1441 agcgctcgat caccttcagg g
//
LOCUS       DAOMEO010000936         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8859_length_1459_cov_2.573362, whole genome shotgun sequence.
ACCESSION   DAOMEO010000936 DAOMEO010000000
VERSION     DAOMEO010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8859_length_1459_cov_2.573362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLH31_17065"
     CDS             <1..430
                     /locus_tag="PLH31_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQR90941.1"
                     /translation="GAERVISHYDQIDAALEAAVAQAGDAPRARLSAYLTASFKPPVL
                     DTQLLAAWLAFWSLVKVNDAIADIHRRTYASYRARLEALLIACDTGVDARLAAIGLTA
                     VVDGLWLELCLDPTVFTPAEAGAIAERLLAGYSPTPVFLD"
     gene            complement(427..666)
                     /locus_tag="PLH31_17070"
     CDS             complement(427..666)
                     /locus_tag="PLH31_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90942.1"
                     /translation="MANEHNYFVRPVSGVWHVTWNDAPDLIGSYSCQAEAQAIAEMLA
                     RHMRVQGKTAKVEVFEPEVRRATWGRSIVSHAAAA"
     gene            complement(842..1459)
                     /locus_tag="PLH31_17075"
                     /pseudo
     CDS             complement(842..1459)
                     /locus_tag="PLH31_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518598.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT          219 a          476 c          492 g          272 t
ORIGIN      
        1 gggggcggag cgcgtgatct cccattatga ccagatcgat gcggcgctgg aggctgcggt
       61 ggcgcaggcg ggagacgccc cgcgcgcccg gctgtcggcc tatctgaccg ccagcttcaa
      121 gccgccggtg ctggacacgc aactgctggc cgcgtggctg gccttctgga gtctggtgaa
      181 ggtcaatgac gccatcgccg atatccaccg gcggacctat gccagctatc gggcgcgact
      241 ggaggcgttg ctgatcgcct gcgacaccgg cgtcgatgcg cggctggcgg ccattggcct
      301 gaccgccgtg gtcgacggtc tgtggctgga actgtgtctg gatccgacgg tctttacccc
      361 ggccgaagcc ggggccatcg cggaacggct gctggccggc tattcgccga caccggtctt
      421 cctggattag gcggcggccg cgtggctgac gatgctgcgg ccccaggtgg cgcggcgaac
      481 ttcgggctcg aacacctcga ccttggcggt ctttccctgc acgcgcatgt ggcgggccag
      541 catctcggcg atcgcctggg cctcggcctg acaggaatag gaccctatca gatccggcgc
      601 gtcattccag gttacgtgcc agacgccgga gacggggcgc acgaagtaat tgtgttcgtt
      661 ggccatggga aactccttgg gctggcttaa gcatcggaca taccgcgtcc gcgtgacagt
      721 gtttcaatgc tgcacggcaa caagcgtgct gcattgcaca ttgcacaggc cgcgccaagg
      781 cgaccgctgg tgaatccagg agcaactttg aggcgaactc gcctgccagg ttagatttgg
      841 tctactccct gaccaccacg ccgcccttca tcaccttggc gaccgaagac agggtggcga
      901 tatcggccag cgggtcgccc gcgacggcga tcatgtcggc atagcgaccc gggctgagcg
      961 cgcccacatc ggccgacttg cccatcagcg cggccgagac agtggtcgct gactggatcg
     1021 cctgcatcgg cgtcatgccg taccgaacca tatagggcag ctgcctggcg ttctggccgt
     1081 gcggatagac gccggcgtcg gtgccatagg cgatcttgac ccccgccttc accgccttgg
     1141 cgaagcccac gcgttgggcg tcggtggtct cggtgttctt gcgcagggtt tcggcgggcc
     1201 agccgttgcg ggttccgacc tcgtcgatga agtcaccatt atagatatcg gccaccagcc
     1261 aggcgccccg ggccttcatc agggcgatcc cctcgtcgtc gatcagcgag ccgtgctaga
     1321 tcgagcgcgc cccggctagc accgccacct tgatgccctc ggccccgtgg gcatgggcgg
     1381 tggcgtaggt tccgcgcttg gccgcggcct cgacggcggc cttgatctgg tcttcggaga
     1441 gttccagttg gcccggttc
//
LOCUS       DAOMEO010000937         1456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8889_length_1456_cov_2.234832, whole genome shotgun sequence.
ACCESSION   DAOMEO010000937 DAOMEO010000000
VERSION     DAOMEO010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8889_length_1456_cov_2.234832"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLH31_17080"
     CDS             <1..428
                     /locus_tag="PLH31_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQR90943.1"
                     /translation="LSLLRRDAPLGQTLRITGKGGKIRIVPVLDAVREAINAYVSALP
                     FVLSPDEPLFRARRGGPLSPRHVQASVQVLRGRLGLSDRVTPHALRHAFATHLLGAGA
                     DLRSIQDLLGHASLSTTQRYTEVDAAGLMAAYARAHPHA"
     gene            602..>1456
                     /locus_tag="PLH31_17085"
     CDS             602..>1456
                     /locus_tag="PLH31_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HQR90944.1"
                     /translation="MKFDDLKISTKVALPAVILTVVALGIVGLGAWQAKEAEAATKVL
                     VEKRAPAELATARFNRQIMGIGYAAYRTVSNTGSSPEAAQASTELDEAYKAAKARLIE
                     IKKADPETTKQTADFGQRLEKIYSSARQGADLALMDADEAAIMVMAVIDPDIAKLNKD
                     VADFTDKHSDETAVMVEDANKASAAGTVFSVLFGVISAGIALLFAIWIGRSKISAPLN
                     NLSKTMETLAQGSVDVDVVGAQRKDEVGAMARSVQVFKDNALALRTAEAAQQRASAET
                     EAERRRNQE"
BASE COUNT          265 a          507 c          453 g          231 t
ORIGIN      
        1 gcctgtcgct gctgcggcgc gacgcgcccc tgggccagac cctgagaatc acgggcaagg
       61 gcggcaagat ccggatcgtg cccgtgctgg acgccgtgcg tgaagcgatc aacgcctatg
      121 tgtccgccct gccctttgtg ctgtcgcccg acgagccgct gtttcgcgcc cggcgcggcg
      181 ggccgctgtc gccgcgccat gtccaggcca gcgtccaggt cctgcgtggc cggctgggcc
      241 tttccgaccg ggtgacccct cacgccctgc gccacgcctt cgccacccac ctgctggggg
      301 ccggcgctga cctgcgctcg atccaggatc tgctgggcca cgcctcgctg tcgaccaccc
      361 agcgctatac cgaggtcgac gccgccggcc tgatggctgc ctacgccagg gcccatcccc
      421 acgcctgagc tgcagccagc ctgagccccc agcctgattc acagcgtcgc tgcgacggac
      481 tgtcgcgaca tgactaacaa aatccgggaa ttaaccgcgt ttttactgcg catggccctt
      541 ttggtctcaa gaaccgacgc ccgtcctccg actggcgggc gttgaggcca tggggttaga
      601 gatgaaattc gacgatctga agatttcgac caaggtggcc ttgccggccg tcatcctgac
      661 cgtggtagcg ctcggtatcg tcggcctggg cgcctggcag gccaaagagg cggaagccgc
      721 caccaaggtt ctggtcgaaa agcgcgcgcc agctgaactg gcaaccgcac gcttcaaccg
      781 ccagatcatg gggatcggct atgcggcgta tcgcacggtg tcgaacaccg gaagctcgcc
      841 cgaggccgcc caggccagca ccgaactgga cgaggcctat aaggcagcca aggcgcgcct
      901 gattgaaatc aagaaggccg atcccgagac caccaagcaa acggccgatt tcggccagcg
      961 cctggaaaag atctattcca gcgcccgcca gggcgccgac ctggccctca tggatgctga
     1021 tgaagccgcc atcatggtca tggccgtgat cgatcccgac atcgccaagc tgaacaagga
     1081 cgtggccgac ttcactgaca agcattccga cgagacggcg gtcatggttg aagacgccaa
     1141 caaggcctca gccgcaggca ccgtcttcag cgtgctgttc ggcgtgatct cggccggtat
     1201 cgccctgctg ttcgccatct ggatcggtcg ctcgaagatt tccgcgcccc tgaacaacct
     1261 gtccaagacc atggaaaccc tggcccaggg ttcggtggac gtcgacgtgg tcggggccca
     1321 gcgcaaggac gaggtcggcg ccatggcccg ctcggttcag gtgttcaagg acaacgccct
     1381 ggccctgcgc accgccgaag ccgcccagca gcgcgccagc gccgagaccg aggccgaacg
     1441 ccgccgcaat caggaa
//
LOCUS       DAOMEO010000938         1455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8893_length_1455_cov_2.217143, whole genome shotgun sequence.
ACCESSION   DAOMEO010000938 DAOMEO010000000
VERSION     DAOMEO010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8893_length_1455_cov_2.217143"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PLH31_17090"
     CDS             complement(<1..839)
                     /locus_tag="PLH31_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HQR90945.1"
                     /translation="MSEPAPLTAFPEPEAEKPKARSRAALLKRLADVVCLPASRVNAF
                     ERAMTADLLVEMLRDAVVEEREKVARRLANLVEMPGTLVRLILRDELSVARALLENSP
                     TLGDADLIDCARHATTEHRRMIAQRRGVGEMVADALVEAGEATVVEVLLRNELVKFSH
                     HTIETVVAMSRDNPRLLPLLLRRAELRPSHAYVMFWWADAEARKLILQRFAVSREILQ
                     DAVGDLFAVASEEGWQDPMSRKALQFIERRQRNRAAIAKSPFDSLDDAIAAAQNGMTR
                     DVA"
     gene            complement(959..>1064)
                     /locus_tag="PLH31_17095"
     CDS             complement(959..>1064)
                     /locus_tag="PLH31_17095"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285406.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HQR90946.1"
                     /translation="GVTSFLEKRTPVYPDKVSTDLPDIWPAWGDHPFS"
     assembly_gap    1065..1074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1075..>1455)
                     /locus_tag="PLH31_17100"
     CDS             complement(<1075..>1455)
                     /locus_tag="PLH31_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR90947.1"
                     /translation="LRMYDSLKPIIAAVNGAAVGVGVTMQLPMDIRLASTDAKFGFVF
                     ARRGINPEAASSWFLPRLVGMQTALEWCYTGRVFGAQEALERGLVRSLHAPEDLLPAA
                     RALAREIADNSAPVSVALTRQLLW"
BASE COUNT          231 a          485 c          510 g          219 t
ORIGIN      
        1 tcggccacat cgcgggtcat gccgttctgc gcggcggcga tggcgtcgtc caggctgtcg
       61 aacggactct tggcgatggc ggcgcggttg cgctggcggc gctcgatgaa ctgcagggcc
      121 ttgcgcgaca tcgggtcctg ccagccctct tcggaggcca cggcgaacag gtcgccgacg
      181 gcgtcctgca ggatctcccg cgacaccgca aagcgttgca ggatcagctt gcgggcctcg
      241 gcgtcggccc accagaacat cacataggca tggctgggac gcagctcggc ccggcgcagc
      301 agcagcggca gcaggcgcgg gttgtcgcgc gacatcgcca ccaccgtctc gatggtgtgg
      361 tggctgaatt tcaccagctc gttgcgtagc agaacttcga cgacggtcgc ctcgccagcc
      421 tcgaccaggg cgtcagcgac catctcgccc acgccgcggc gctgggcgat catccgccgg
      481 tgctcggtgg tggcgtggcg ggcgcagtcg atcaggtcgg cgtcgcccag ggtgggggaa
      541 ttttccagca gggcgcgggc caccgacagc tcgtcgcgca ggatcaggcg caccagcgtg
      601 cccggcatct cgaccagatt ggccaggcgg cgggccacct tctcgcgctc ctcgaccacc
      661 gcgtcgcgca gcatctcgac cagcaggtcg gcggtcatgg cccgttcgaa ggcgttgacc
      721 cggctggcgg gcaggcagac cacgtcggcc aggcgcttga gcagggccgc ccgcgaacgg
      781 gcctttggct tctcggcctc aggctctgga aacgcggtaa ggggcgcggg ttcgctcatg
      841 gtcatcctga cggtcgcaac agccgtcaca ccgaactgat cagatcgtca ttaacgtccg
      901 atgagcggtg ggtaaggcgc cccttggata tgggaacgcc tcgccgtgac caaaagcctc
      961 agctgaatgg atggtcgccc cacgccggcc agatgtccgg caggtcggtc gagaccttgt
     1021 cgggatagac cggcgtgcgc ttttccagga acgaggtgac ccctnnnnnn nnnngccaca
     1081 gcaactggcg ggtcagggcc acggaaacgg gagcgctgtt gtcggcgatc tcgcgggcca
     1141 gggcgcgggc ggcgggcagc aggtcttcgg gcgcgtgcag cgagcggacc aggccgcgct
     1201 ccagcgcttc ctgcgcgccg aacacccggc cggtgtagca ccactccagc gccgtctgca
     1261 tcccgaccag gcgcggcagg aaccaggacg aggccgcctc gggattgatc ccgcgccgcg
     1321 cgaacacgaa gccgaacttg gcgtcggtcg aggccaggcg gatatccatc ggcagctgca
     1381 tggtcacgcc cacgccgacc gccgcgccat tcacggcggc gatgatgggt ttgaggctgt
     1441 catacatgcg aagcg
//
LOCUS       DAOMEO010000939         1453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8907_length_1453_cov_1.979256, whole genome shotgun sequence.
ACCESSION   DAOMEO010000939 DAOMEO010000000
VERSION     DAOMEO010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8907_length_1453_cov_1.979256"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /locus_tag="PLH31_17105"
     CDS             complement(<1..82)
                     /locus_tag="PLH31_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQR90948.1"
                     /translation="MSLKGKTLFVTGASRGIGLAIALRAAR"
     gene            complement(79..597)
                     /locus_tag="PLH31_17110"
     CDS             complement(79..597)
                     /locus_tag="PLH31_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90949.1"
                     /translation="MESRSVLRLAAVATIIGAAIDIVAPFVIYPRLVQPWPHLVYVVI
                     DLLLLCGMLGVRSVSGRSTGPLALAGFVLAVLGVLLVRTSSAEIFGEASYMIASSVWS
                     IGMVLWSVDLLRARGPFRLAAGLWIATLVIGLAGVVLKDHGPIAHVAKMSFILGFIAA
                     AVDLFKVRGDPA"
     gene            complement(597..>1453)
                     /locus_tag="PLH31_17115"
     CDS             complement(597..>1453)
                     /locus_tag="PLH31_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90950.1"
                     /translation="VETARALAEAGAEVIIAVRKPDLAEAAVAEVNKTAKGAKASWSM
                     LDLSSFRSIRAFAERWGDRPLNILINNAGVMACPLAYTEDGLEMQIGTNHFGHYLLSV
                     LLAPNLIAGARASGKTSRLVSLSSIGHRRSGVNFDDPHYKTRDYDKWESYGQAKSANS
                     LFAVGFNKRFKDQGVFANAVMPGGIMTPLQRHLPIEEQIALGWIDEHGKVRDGFKTTE
                     EGASTSVWAAVGDELEGVGGLYLEDLAEAPPWTKDKPWAGVMPHALDPEAAERLWALS
                     VETTGAGA"
BASE COUNT          255 a          484 c          492 g          222 t
ORIGIN      
        1 cgcgcgcggc ccgcagggcg atggccaggc cgatgccgcg cgaggcgccg gtgacgaaca
       61 gggtctttcc cttgaggctc atgcagggtc tccacggact ttgaacagat cgacggcggc
      121 ggcgatgaag ccaagaatga agctcatctt cgccacatgc gcgatcggac cgtgatcctt
      181 caggaccacg cccgcaaggc cgatgacgag ggtggcgatc cacaggccgg cggcgaggcg
      241 gaacggcccc ctggcgcgca gcagatccac gctccagagg accatgccga tcgaccagac
      301 gctggaggcg atcatgtatg aggcctcgcc gaagatctcg gccgacgagg ttcgcaccag
      361 caacacgccc agcaccgcca gaacaaagcc ggccagggcg aggggcccgg tcgaccgtcc
      421 gctgacggag cgaacgccca gcattccgca gagcagcaac aggtcgatga ccacatagac
      481 caggtgcggc cacggctgga ccaggcgcgg atagatcacg aacggcgcca cgatatcgat
      541 ggccgcgccg atgatcgtgg cgacagcggc cagccgcagg acggagcggc tttccatcag
      601 gcgccggccc ccgtggtttc caccgacagg gcccacaggc gctcggcggc ttcgggatca
      661 agggcgtgag gcatgacgcc ggcccagggc ttgtcttttg tccacggcgg ggcctcagcc
      721 aggtcttcca gatagagccc gcccacgcct tccagttcgt cgcccacggc ggcccagacg
      781 ctggtcgagg cgccctcctc cgtcgtcttg aagccgtcgc gcaccttgcc atgctcgtcg
      841 atccagccca gggcgatctg ctcctcgatc ggcaggtggc gctgcagcgg agtcatgatc
      901 ccacccggca tcacggcgtt ggcgaacacg ccctgatcct tgaagcgctt gttgaaaccg
      961 acggcgaaga gactgttggc gctcttggcc tggccgtagc tttcccactt gtcgtagtcg
     1021 cgggtcttgt agtgcgggtc atcgaagttc acgcccgagc ggcggtggcc gatggacgac
     1081 agcgacacca gccgcgaggt cttgccggaa gccctggcgc ccgcgatcag gttgggcgcc
     1141 agtagcactg acagcagata gtggccgaaa tgattggtgc caatctgcat ctccagcccg
     1201 tcctcggtat aggcgagcgg gcaggccatg acgccggcat tgttgatcag gatgttcagc
     1261 ggcctgtcgc cccagcgctc ggcgaaggcg cggatcgacc tgaagctgga gaggtccagc
     1321 atcgaccacg acgccttggc gcctttcgcg gtcttgttga cctcggcgac ggcggcttcg
     1381 gccaggtcgg gcttgcggac ggcgatgatc acctcggcgc cggcctcggc cagggcgcgg
     1441 gcggtctcga cgc
//
LOCUS       DAOMEO010000940         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8909_length_1452_cov_3.369363, whole genome shotgun sequence.
ACCESSION   DAOMEO010000940 DAOMEO010000000
VERSION     DAOMEO010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8909_length_1452_cov_3.369363"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLH31_17120"
     CDS             <1..460
                     /locus_tag="PLH31_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQR90951.1"
                     /translation="RKIYLDLTFWNELESKFGAKGEFARAYVISHEVGHHVQTLLGAS
                     QAARRMGAQGAESGTVRLELQADCYAGVWASKAGETSGGQIVIRPADIEDGLGAAAAV
                     GDDTIQSRTQGRVVPDSFTHGTSEQRMRWFTRGYERGDPAVCDTFGASRL"
     regulatory      498..603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            676..>1452
                     /locus_tag="PLH31_17125"
     CDS             676..>1452
                     /locus_tag="PLH31_17125"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HQR90952.1"
                     /translation="MNVQTTIKAVAETISTGSIPGSRKVYQAGELFPDIRVPFREVAV
                     HPSANEPPVTVYDPSGPYSDPTVTIDIEKGLERTREAFVVARGDVEVVAQPRAVKPED
                     NGFAQGKHLAPQFPAVGRTIYRGKPGALITQYEYANAGKITAEMEYVAIRENLRREQD
                     RPCVRDGDDFGASIPDFVTPEFVRQEVARGRAIIPANINHGELEPMAIGRNFLVKINA
                     NIGNSAVLSTVADEVDKLVWATRWGADTVMDLSTGRNIHNI"
BASE COUNT          249 a          502 c          479 g          222 t
ORIGIN      
        1 tcgcaagatc tatctggacc tgaccttctg gaacgagctt gagtcgaagt tcggcgccaa
       61 gggcgagttc gcccgggcct atgtgatcag ccacgaggtc ggccaccacg tgcagaccct
      121 gctgggcgct tcccaggcag cccggcgcat gggcgcgcag ggcgccgaga gcggcacggt
      181 gcgactggag ctgcaggccg attgctatgc cggggtctgg gcgtcgaagg ctggcgagac
      241 ctcgggcggc cagatcgtga tccgtcccgc cgacatcgag gatggcctgg gcgcggcggc
      301 ggcggtgggc gacgacacca tccagtcgcg cacccagggt cgggtggtcc ccgacagctt
      361 cacccacggc accagcgagc agcggatgcg gtggttcacg cgcggctatg agcgcggcga
      421 tccggcggtg tgcgacacgt tcggcgcgtc gcggctgtaa gggtgtcttg acccacacgt
      481 cagtgcgcct agtgtccccc cgttcaccgg ggggtcgcta gcgcggctga gattgggctt
      541 tcgtcctgac ccgtcgaacc tgatccgggt catgccggcg aagggaggga acgcggtcgg
      601 ggcttacagt ccgtccgtta gaaccgactt agcacgcttg tccgggtctc cttaatgctt
      661 gaggagcgcc cgaaaatgaa cgtccagacc accatcaagg ccgtcgccga gaccatctcg
      721 accggctcca tccccggctc ccgcaaggtc tatcaggccg gcgagctgtt ccccgacatc
      781 cgcgtgccgt tccgcgaggt ggccgtccac ccttcggcca acgagccgcc ggtcacggtc
      841 tatgacccgt ccggtcccta cagcgacccg accgtcacga tcgacatcga aaagggcctg
      901 gagcgcaccc gcgaagcctt cgtggtcgct cgcggcgacg tcgaggtggt ggcgcagccc
      961 cgcgcggtga agcccgagga caacggcttc gcccagggca agcacctggc cccgcagttc
     1021 cccgccgtgg gtcgcaccat ctatcgcggc aagccgggcg cgctgattac ccagtacgaa
     1081 tacgccaacg ccggcaagat caccgccgag atggaatatg tggccatccg cgaaaacctg
     1141 cgccgcgagc aggaccgccc gtgcgtgcgc gacggcgacg atttcggcgc ctcgatcccc
     1201 gacttcgtga cccccgagtt cgtgcgccag gaagtggccc gcggccgcgc catcatcccg
     1261 gccaacatca accacggcga gctggagccg atggcgatcg gccgcaactt cctggtcaag
     1321 atcaacgcca atatcggcaa ctcggcggtg ctcagcaccg tcgccgacga ggtcgacaag
     1381 ctggtctggg ccacccgttg gggcgccgac acggtcatgg acctgtcgac tggccgcaac
     1441 atccacaaca tc
//
LOCUS       DAOMEO010000941         1452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8911_length_1452_cov_1.952756, whole genome shotgun sequence.
ACCESSION   DAOMEO010000941 DAOMEO010000000
VERSION     DAOMEO010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8911_length_1452_cov_1.952756"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="rlmB"
                     /locus_tag="PLH31_17130"
     CDS             <1..773
                     /gene="rlmB"
                     /locus_tag="PLH31_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQR90953.1"
                     /translation="DFSHKPRNKQSFSKGGSDDKDWIWGIHAVEAALANPARPAPKRL
                     LATPDRAKRLAPNWAKLPCLQIMEGGDIARQLPQGAAHQGLALKIDEPEPLSIAELGT
                     PAQGVIVMLDQITDPQNVGAIFRSAAAFGVRGAILQDRHAPTLSGVLAKTAVGAIDKV
                     PYSRVVNLSRALEELADMGWRAIGLAGEAEETLDIVLDGTPTVLVLGSEGEGVRRLVA
                     EHCDAMGKIAMPGGFESLNVSAAAAIALYEASRVSRKG"
     gene            954..>1452
                     /locus_tag="PLH31_17135"
     CDS             954..>1452
                     /locus_tag="PLH31_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90954.1"
                     /translation="MAGCGAGDVDTTPYAMTETRAETAPDLMGAPPAPDAGSAPDGLL
                     GAPTPAAAPVPVTVQPSPNKSSTLKTWRRPDGTLVTAMRPIPNPKERRAVRYAPRASS
                     APANRAAVASAPVAKPVAKPVTVAAAPVLKPVAPITPVAPLAKAPTPAIPAAVATKPA
                     SKLDQL"
BASE COUNT          240 a          500 c          471 g          241 t
ORIGIN      
        1 gtgattttag tcacaagcca cggaataaac agagcttttc caagggcgga agcgacgata
       61 aggactggat ttgggggatt cacgctgtcg aggcggccct ggccaacccc gccagacccg
      121 cgccgaagcg cctgttggcc accccggacc gggccaagcg actcgcgccg aactgggcca
      181 agctgccctg cctgcagatc atggagggcg gcgatatcgc ccgccagctc ccgcagggcg
      241 ccgcgcacca gggcctggcc ctgaagatcg acgaacctga acccctgtcg atcgccgaac
      301 tggggacgcc agcccagggc gtgatcgtca tgctggacca gatcaccgac cctcagaacg
      361 tcggcgccat cttccgctcg gccgccgcct tcggggttcg cggcgccatc ttgcaggatc
      421 gccacgcccc aacgctctca ggcgtcctgg ccaagacggc ggtcggcgcc atcgacaagg
      481 tgccctattc gcgcgtcgtc aatctgtcac gcgcgcttga ggaactggcc gacatgggct
      541 ggcgggccat cggcctggcc ggtgaggccg aagaaacgct cgatatcgtg ctcgatggaa
      601 cgccgaccgt acttgttctg ggttcggaag gtgaaggagt ccgcagattg gtcgcagagc
      661 attgtgatgc catgggaaaa attgccatgc cgggcggttt cgagagcctg aatgtgtcag
      721 ccgcggcggc aatcgcgctc tacgaggcgt cgcgagtttc gcgtaaggga tgaaatgagt
      781 tcgtgaatct tctttaaccg ttttgatttc gtcgcaagct catgcttctc tcatggacaa
      841 ggttccgcga gtgctgctcg ctggaacact cttgcatggg atcgggggac ccgatgtttg
      901 gacgtcatac gcgtttggct gctcttggcg cttcggcgct gggcgttttg gtgatggctg
      961 gttgcggcgc aggggatgtg gacaccacgc cctacgccat gaccgagacc cgcgccgaga
     1021 ccgcgccgga tctgatgggc gcgccgcccg cgcctgacgc cggctcggcg cccgacgggc
     1081 tgctgggcgc gccaacgccg gccgcagcgc ctgtgccggt gaccgtgcag ccctcaccga
     1141 acaagagctc gacgctgaag acctggcgtc gcccggacgg gacgctggtg accgccatgc
     1201 ggcccatccc caatcccaag gagcgccgcg ccgttcgcta tgcgccgcgc gcatcctccg
     1261 cgcccgccaa tcgcgctgct gttgcatccg ccccggtcgc caagcccgtg gccaagccgg
     1321 tcaccgtcgc ggccgcgccc gtcctcaagc cggtggcccc gatcacgccg gtcgcgccgc
     1381 tggccaaggc cccgacgccc gccatcccag ccgccgtcgc gaccaagccg gccagcaagc
     1441 tcgaccagtt gc
//
LOCUS       DAOMEO010000942         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8921_length_1450_cov_2.204301, whole genome shotgun sequence.
ACCESSION   DAOMEO010000942 DAOMEO010000000
VERSION     DAOMEO010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_1450_cov_2.204301"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLH31_17140"
     CDS             <1..427
                     /locus_tag="PLH31_17140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR90955.1"
                     /translation="ATTAPASAAPATTAGQTFLAANAKVPGVVVLPSGLQYKILTSGP
                     KTGPSPKPGDIIKVHYEGKLLDGTVFDSSFARKQSAIMPLEGLVQAWLEALPMMKVGD
                     EWVLYVPPALGYGDRDVGPIPAGSVMTFRLQLLGMLAVD"
     gene            complement(543..>1450)
                     /gene="rutA"
                     /locus_tag="PLH31_17145"
     CDS             complement(543..>1450)
                     /gene="rutA"
                     /locus_tag="PLH31_17145"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HQR90956.1"
                     /translation="GFGGKTQFWEHNLESFTLMAGLAAVTSKIKIFATVATLTIPPAI
                     AARMASTIDSIAPGRFGLNLVTGWQKAEYSQMGLWPGEAHYTDRYNYLAEYTTVLKDL
                     LETGVSDFKGQYFQMDDCRVSPHPKETKLICAGSSNEGLAFTAQYADYSFALGKGTNT
                     PAAFASVNDRLKAAADKTGRDVASFILFMIIADETDEKAFAKWRLYRDGADQEALAWL
                     TQQAAPNAAAGATTNTTQLAAPESAVNLNMGTLVGSYESVARMLDEVAAVPGTAGVLL
                     TFDDFVEGVENFGTRIQPLMKSRQK"
BASE COUNT          223 a          488 c          482 g          257 t
ORIGIN      
        1 cgcgacgacg gcgccggcca gcgccgcgcc cgccacgacg gcgggtcaga ccttcctggc
       61 cgccaacgcc aaggttcccg gcgtggtcgt tctgccctcg ggcctgcagt acaagatcct
      121 gacctcgggc cccaaaaccg gcccttcgcc caagcccggc gacatcatca aggtccacta
      181 tgaaggcaag ctgctggacg gcacggtctt cgacagctcg ttcgcgcgca agcaatcggc
      241 catcatgccg ctcgaaggcc tggtccaggc ctggctggag gccctgccga tgatgaaggt
      301 cggcgacgag tgggtgctct atgtgccgcc cgcgctgggc tacggagacc gtgacgtcgg
      361 cccgatcccg gctggcagcg tgatgacgtt ccggctgcag ctgctgggca tgctggcggt
      421 ggattgatcc aatctccctt cccccttgat gggggaaggg ccggggatgg gggtgacgcg
      481 gcggttcaac cggcgagcgc cctatcaccc ccacccaacc ctcccccatc aagggggagg
      541 gcttattttt gccggctctt catcagcggc tggatccgcg tcccgaagtt ctccaccccc
      601 tcgacgaagt catcaaaggt cagcagcacc ccggccgtgc ccggcacggc ggccacctcg
      661 tccagcatcc gcgccacgct ctcatagctc cccaccaggg tgcccatgtt gagattgacc
      721 gccgactccg gcgcagccag ctgggtggtg ttggtggtcg cgcccgccgc cgcgttgggg
      781 gccgcctgct gggtcagcca ggccagggcc tcctgatcgg cgccgtcgcg atagagccgc
      841 cacttggcaa aagccttctc gtcggtctcg tcggcgatga tcatgaacag gatgaagctg
      901 gccacgtccc gcccggtctt gtcggcggcg gccttcaggc ggtcattgac cgaggcgaag
      961 gcggcggggg tgttggtgcc cttgcccagg gcgaagctgt agtcggcata ttgcgccgtg
     1021 aaggccaggc cctcgttcga cgagccggcg cagatcagct tggtttcctt ggggtgcggg
     1081 ctgacccggc agtcgtccat ctggaaatac tggcccttga agtccgaaac cccggtctcc
     1141 agcaggtcct tcagcacggt cgtgtattcg gccaggtagt tatagcggtc ggtatagtgc
     1201 gcctcgcccg gccacaggcc catctggctg tattcggcct tctgccagcc ggtcaccagg
     1261 ttcaggccaa accggcccgg ggcgatggag tcgatggtcg aggccatgcg cgcggcgatg
     1321 gccggcggga tggtcagggt ggcgacggtg gcgaagatct tgatcttgct ggtcacggcc
     1381 gccaggccgg ccatcagggt gaagctctcg agattatgct cccagaactg cgtcttgccg
     1441 ccaaacccgc
//
LOCUS       DAOMEO010000943         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8942_length_1447_cov_2.273707, whole genome shotgun sequence.
ACCESSION   DAOMEO010000943 DAOMEO010000000
VERSION     DAOMEO010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8942_length_1447_cov_2.273707"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..>1447)
                     /locus_tag="PLH31_17150"
     CDS             complement(288..>1447)
                     /locus_tag="PLH31_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQR90957.1"
                     /translation="IGTASLVAMGCIMVRQCHSNTCPVGVCTQDEALRAKFTGTPEKV
                     INLFSFIAEEVREILAGLGFRSLQEIVGRTDLLAQVSRGGEHLDDLDLNPLLVRADPG
                     QNKPYNTVVGRNEVPDTLDAQIVRDAAPLLERGEKMQLTYTVRSTARTIGTRTSSHIV
                     RKFGMKGLPAGHLTVQLKGSAGQSLGAFAVQGLRIELTGEANDYVGKGLSGATIVIKP
                     SRHLSSPESNALLGNTVLFGATSGRLFAAGQAGERFAVRNSGATTVVEGCGANGCEYM
                     TGGQVVILGPTGGNFGAGMTGGMAFVLDQHGRFEKAFNPESIVVQRLASPYWEGVLRS
                     LVAEHARETDSAFAGSLLRDWDRVRDLFWQVCPKEMVSRLPQPLAAEEVAA"
BASE COUNT          230 a          507 c          473 g          237 t
ORIGIN      
        1 ccgtcggggg cctagctacc ggagcgggtt aaaaccccgc cagcccggcg atgcttccgg
       61 ataacgatca cgcggagcgt ctgtcttcgt cgaaacggta ttcaactaat ccctcatccg
      121 ggcgaggccc ggacgctccg cagcccttcc caccaactcg gcggtttccg cgcgaggctg
      181 tgaaaccgtg tccgccagac ttgccccctc cgcgcctacg gcgctcctcc cccgtagggg
      241 gaagatgacg tgcgcggagg gcgccggtcc tccgcgcccg ggaacgtcta ggctgcgacc
      301 tcttccgccg ccagcggctg cggcaaccgc gacaccatct ccttgggaca cacctgccag
      361 aacaggtcgc gcacccggtc ccagtcccgt agcaggctgc ctgcgaaggc cgagtcggtt
      421 tcgcgggcgt gttcggccac cagcgaccgc agcacgccct cccaataggg cgaggccagg
      481 cgctggacga cgatgctttc cggattgaag gccttctcga accggccgtg ctggtcgaga
      541 acaaaggcca tgccgccggt catgccggcc ccgaaattgc cgcccgtcgg acccaggatc
      601 accacctggc cgcccgtcat atattcgcag ccattggcgc cgcagccctc gaccaccgta
      661 gtggcgcccg agttgcggac cgcaaagcgc tcgcccgcct gtcccgccgc gaacagccgg
      721 ccggaagtgg cgccaaacag cacggtgttg cccagcaggg cgttgctttc cggcgacgac
      781 agatggcgcg agggcttgat gacgatcgtc gcgcccgaca ggcccttgcc aacatagtcg
      841 ttggcctcgc cggtcagttc gatccgcagg ccctggaccg cgaaggcgcc caggctctgg
      901 ccggccgaac ccttgagctg gacggtcaga tggccggcgg gcaggccctt catgccaaac
      961 ttgcgcacga tgtggcttga ggtccgcgtg ccgatcgtgc gcgccgtgct gcgaaccgta
     1021 taggtcagct gcatcttctc gccgcgctcc agcagggggg cggcgtcgcg aacgatctgg
     1081 gcgtccaggg tgtcgggcac ttcgttgcga ccgaccacgg tgttgtaggg cttgttctgg
     1141 ccaggatcgg cgcgaaccag cagcgggttc aggtcaaggt cgtccaggtg ctcgccgccg
     1201 cggctgacct gggccaagag gtcggtgcgg ccgacgattt cctgcaggct cctgaagccc
     1261 agtccggcca ggatctcgcg cacttcctct gcgatgaagc tgaagaggtt gatgaccttt
     1321 tccggcgtgc ccgtgaactt ggcgcgcagg gcctcgtcct gggtgcacac ccccaccggg
     1381 caggtgttgg aatggcactg gcgcaccatg atgcagccca tcgccacgag cgaggccgtg
     1441 ccgatgc
//
LOCUS       DAOMEO010000944         1447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8945_length_1447_cov_1.882902, whole genome shotgun sequence.
ACCESSION   DAOMEO010000944 DAOMEO010000000
VERSION     DAOMEO010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8945_length_1447_cov_1.882902"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..410)
                     /gene="fliE"
                     /locus_tag="PLH31_17155"
     CDS             complement(102..410)
                     /gene="fliE"
                     /locus_tag="PLH31_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HQR90958.1"
                     /translation="MITALAAAKAYASQGAMSVGSIGGGASAAKGMDFGDLLKNAMND
                     VTQSSKAAEMKVAEQVAGKAELIDVVTAISSAEASLETVMAVRDQVISAYQEIMRMPI
                     "
     gene            complement(421..840)
                     /gene="flgC"
                     /locus_tag="PLH31_17160"
     CDS             complement(421..840)
                     /gene="flgC"
                     /locus_tag="PLH31_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HQR90959.1"
                     /translation="MPEIKSNSLSTMAVATSALRAQQGRMRVIAENIANANSTARVAG
                     GDPYRRQVPVFKPTKIDGAEGVQMLRVEPDQSDFKTEYDPGNPAADDKGYVKLPNVNS
                     LVEALDMKEAQRAYEANLNVIETARAMQSRTLDVLRK"
     gene            complement(851..1297)
                     /gene="flgB"
                     /locus_tag="PLH31_17165"
     CDS             complement(851..1297)
                     /gene="flgB"
                     /locus_tag="PLH31_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HQR90960.1"
                     /translation="MGPDDIPLFGMLKSRLGYLSERQKVIAQNVANASTPGYQPRDLK
                     AYSFQASLMNQGSGQAPYRGGRVAPTGGVPMMATSASHMAPSRAPSAWRATAGADTET
                     TLDGNAVNLEDQMLKMSDARMNYDAAVGFYQKSMTMLRMSARRPGG"
BASE COUNT          231 a          452 c          493 g          271 t
ORIGIN      
        1 tcccgcacct gcggcggaca gaggggttcc cgccacaagg gcgggaatga cggcgtgctt
       61 tgggtagaga cagaaggtga agcgggcgac gaaaacagcc gctagatcgg catccgcatg
      121 atctcttgat acgccgagat cacctgatcg cgcaccgcca tcacggtttc gaggctggcc
      181 tcggccgacg agatcgcggt caccacgtca atgagctcag ccttgccggc cacctgctcg
      241 gcgaccttca tttcggcggc cttggagctc tgggtgacgt cattcatggc gttcttcagg
      301 aggtccccga agtccatgcc cttggcggcg gaggcgccgc cgccgatcga gccgaccgac
      361 atggcgcctt gcgaagcata ggccttggcg gcggcgagcg cggtgatcat cggtcagccc
      421 ctatttgcgc aggacatcga gggtgcgcga ctgcatggcg cgcgccgttt cgatgacgtt
      481 gagattggcc tcgtaggcgc gctgcgcctc tttcatgtcc agcgcctcga ccagcgagtt
      541 gacgttgggc agcttgacat agcccttgtc gtcggcggcc ggattgcccg ggtcgtattc
      601 ggtcttgaag tcgctctggt cgggctcgac gcgcagcatc tgcacgccct cggcgccgtc
      661 gatcttggtg ggcttgaaca ccggcacctg gcgacgatac gggtcgccgc cggcgacgcg
      721 cgcggtggag ttggcgttgg cgatgttctc ggcgatcacc cgcatgcggc cctgctgggc
      781 gcgcagggcc gaggtggcca cggccatggt cgacagcgaa ttggatttga tctcgggcat
      841 cgcctaatcc tcagccgccg ggccggcgcg cggacatccg caacatggtc atggacttct
      901 ggtagaagcc gacggcggcg tcatagttca tgcgcgcgtc ggacatcttg agcatctggt
      961 cctcgaggtt cacggcgttg ccgtcgaggg tggtctcggt gtcggcgccg gccgtggcgc
     1021 gccaggcgct gggcgccctg gacggagcca tgtgcgaggc cgaggtggcc atcatcggca
     1081 cgccgccggt cggcgccacc ctgccgccgc gatagggcgc ctggcccgaa ccctggttca
     1141 tcagcgaggc ctgaaagctg taggccttca ggtcgcgcgg ctgatagccg ggcgtgctgg
     1201 cgttggccac gttctgggcg atgaccttct gccgctcgga caaatagccg agccggctct
     1261 tgagcatgcc aaaaagggga atgtcgtcgg gacccatggc gatcatggtg aagaaacagg
     1321 gttaatccgg cattaagaag cctcaggcac accagtatcg acgcagcttt cgcctgcgcc
     1381 atcttgtctc gcttagctgg tcgatacggg cgtcatggac atcctggaaa tcatccgcgc
     1441 catcgcc
//
LOCUS       DAOMEO010000945         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8951_length_1446_cov_2.639827, whole genome shotgun sequence.
ACCESSION   DAOMEO010000945 DAOMEO010000000
VERSION     DAOMEO010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8951_length_1446_cov_2.639827"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..739
                     /locus_tag="PLH31_17170"
     CDS             137..739
                     /locus_tag="PLH31_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR90961.1"
                     /translation="MAEKARRSPRQARAEATVEAIFDATFQLLEAGGLERLTTNHIAQ
                     RAGVSIGTLYQYFGGKQEILAAMAQRRAEVARGRIAETVIAAPQVGSVRPIVQALMAT
                     FEGSPETRKALLDALFDRGGDAVMLHHHQAFLAAIAGKAQLNLDLSPEQLFVLTHAVA
                     SLLRAVAAEPDLNLSPTVLEDEFVLLMESYLSALIARERR"
     gene            complement(749..>1446)
                     /locus_tag="PLH31_17175"
     CDS             complement(749..>1446)
                     /locus_tag="PLH31_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR90962.1"
                     /translation="KCQEGEFPREAFEAAGLPHLKIAGQKGWHGVAIASRLPFEDVPT
                     LMNCREGHARCVAVKVAGVDIQNFYIPAGGDTPDRIGNPKFDHKLDFYETLTAALKKR
                     DPKDPLIVTGDLNIAPGENDVWSHRQMLKVVSHTPAELEAFDAMIKSMGLLDLPRLAT
                     PEPEKLFSWWSYRAADFRKSNRGLRLDHILASPGLRDAAIIDGKVSAKVHDTVREWER
                     PSDHAPVTADLRL"
BASE COUNT          231 a          468 c          491 g          256 t
ORIGIN      
        1 tgctcgatcg tgctggtcat gttcaatccc gtttggcgga tggcggcccc cgccagacca
       61 tgagaatatg taagcattgc tcatgtttct actcgccttg cccggcgagc gagccgaccg
      121 cgaaggaagc tggcaagtgg ctgaaaaagc gagacgatcg cccaggcagg cccgggcgga
      181 ggccacggtg gaggcgattt ttgacgccac ctttcaactt ctggaggccg gcggactgga
      241 gcgcctgacc accaaccaca tcgcccagcg ggccggggtc agcattggca cgctgtacca
      301 gtatttcggc ggcaagcagg aaatcctggc cgccatggcc cagcgccggg ccgaggtggc
      361 gcgcggccgg atcgccgaga cggtgatcgc ggcgccgcag gtgggtagcg tgcggcccat
      421 cgtccaggcg ttgatggcca ccttcgaggg ttcgcccgaa actcggaaag ccctgctgga
      481 cgccctgttc gatcgcggcg gcgacgcggt gatgctgcat catcaccagg ccttcctcgc
      541 cgccatcgcc ggcaaggcgc agctcaatct ggatctgtcg ccggagcagc tgttcgtgct
      601 gacccacgcg gtcgccagcc tgctgcgcgc cgtggcggcc gaacccgacc tgaatctgtc
      661 gcccaccgtg ctggaggacg agttcgtgct gctgatggag agctatctgt ccgcgctgat
      721 tgcgcgggaa cgacgctagg ccgaaatcct aaagcctcaa atccgccgtc accggcgcgt
      781 ggtcgctggg gcgctcccat tcgcggaccg tgtcatgcac cttggccgag accttgccgt
      841 cgatgatggc cgcgtcgcgc agcccggggc tggccaggat atgatccagg cgcaggccgc
      901 ggttggactt gcggaagtcg gcggcgcgat agctccacca ggaaaacagc ttttccggct
      961 ccggcgtggc caggcgcggc aggtccagca ggcccatcga tttgatcatc gcatcgaagg
     1021 cctccagttc cgccggcgtg tggctgacca ccttcagcat ctggcggtgg ctccagacgt
     1081 cgttctcgcc cggggcgata ttcaggtcgc cggtgacgat cagcgggtcc ttcgggtcac
     1141 gctttttcag ggcggcggtc agggtctcgt agaagtccag cttgtggtcg aacttgggat
     1201 tgccgatgcg gtcgggcgtg tcgccgccgg ccgggatgta gaagttctgg atgtcgaccc
     1261 cagccacctt gaccgccacg cagcgggcgt ggccctcgcg gcagttcatc agggtgggaa
     1321 catcctcgaa cggcaggcgc gaggcgatgg ccacgccgtg ccagcccttt tgcccggcga
     1381 tcttcaggtg cggcagaccg gcggcctcga aggcctcgcg cggaaactcg ccctcctggc
     1441 acttga
//
LOCUS       DAOMEO010000946         1446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8956_length_1446_cov_2.186197, whole genome shotgun sequence.
ACCESSION   DAOMEO010000946 DAOMEO010000000
VERSION     DAOMEO010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_1446_cov_2.186197"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1199)
                     /gene="coaBC"
                     /locus_tag="PLH31_17180"
     CDS             complement(3..1199)
                     /gene="coaBC"
                     /locus_tag="PLH31_17180"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQR90963.1"
                     /translation="MSDKRVLLIVGGGIAAYKALELTRLLRKAGVGVRPILTRAGAEF
                     VTPLSLGSLAEDRVYDDLFSLTDEAEMGHIQLSRSADLVVVAPATADLIAKAAHGLAG
                     DLASTTLLATDKPVLMAPAMNVRMWLHPATQRNIATLKADGVTIVGPDEGAMACGEFG
                     PGRLAEPETIFAAVMDALEGPAAKPLAGKRALVTAGPTFEPIDPVRGITNRSSGKQGF
                     AIAEALAQLGAEVTLIAGPVFLPTPPGVTRIDVETARQMLAASQAALPADVGVFVAAV
                     ADWRVDEAFGTKLKKEKGGPPALTFVENPDILAAVSATGPMRPKLVVGFAAETNDVEM
                     HARAKLSRKGCDWIIANDVTEPGVMGGEDNAVLLVTRKKTERWDRASKNEVARAIALR
                     IAEALA"
BASE COUNT          215 a          485 c          507 g          239 t
ORIGIN      
        1 ccctaggcga gagcttccgc gatcctcagg gcgatggcgc gcgccacctc gttcttggac
       61 gcacggtccc agcgctcggt cttcttgcgc gtgaccagca gtacggcatt gtcttcgccg
      121 cccatgacgc caggctcggt gacgtcattg gcgatgatcc agtcgcagcc cttgcgcgac
      181 agcttggcgc gggcgtgcat ctccacatca ttggtctcgg ccgcgaagcc gaccaccagc
      241 ttgggccgca tcggacccgt ggccgagacg gcggccagga tgtcggggtt ttcaacgaag
      301 gtcagggctg gcggtccgcc cttttccttc ttcagcttgg tcccgaaggc ctcgtccacc
      361 cgccagtcgg cgacggcggc cacgaacacc cccacatcgg ccggcagggc ggcctggctg
      421 gcggccagca tctggcgagc ggtctcgaca tcgatgcggg tcacgccagg cggggtgggc
      481 aggaagaccg ggccggcgat cagggtgacc tcggcgccca gttgcgccag cgcttcggcg
      541 atcgcaaagc cctgcttgcc gctggagcgg ttggtgatgc cccgaaccgg gtcgatgggc
      601 tcaaaggtgg ggccggcggt gaccagcgcg cgcttgccgg ccaggggttt ggcggcgggg
      661 ccttcaagcg cgtccatgac cgcggcgaag atggtctccg gctcggccag acgcccgggg
      721 ccaaactcgc cgcaggccat ggccccctcg tcgggtccaa cgatcgtgac gccgtcggcc
      781 ttcagggtgg cgatattgcg ctgggtggcc gggtgcagcc acatccgcac gttcatggcc
      841 ggggccatca gcactggctt gtcggtggcc agcagggtgg tggaggccag gtcgccggcc
      901 aggccatggg cggccttggc gatcaggtca gcggtcgccg gcgccaccac caccaggtcg
      961 gccgagcgcg acagctggat gtggcccatc tcggcctcgt cggtcaggga gaacaggtca
     1021 tcatagaccc tgtcttcggc cagcgagccc agcgacagcg gcgtcacgaa ctcggcgccg
     1081 gcccgggtca ggatcgggcg cacgcccacc ccggccttgc gcagcaggcg cgtcagctcc
     1141 agcgccttgt aggccgcgat gccgccgcca acgatcagaa gaacccgctt gtcgctcacg
     1201 ccggttggtc tccgcattgc ttccgccctt atggcccaag cctggcgaac agacaaagga
     1261 caagctcatg acggactcga ctcgcgttcc gctttcgttc attaatcggt aaggttgaac
     1321 tgatggggtc aggggatcat gaggttgaac agatttcaca tcgctgcaac ccgctggcgg
     1381 ggcgcggccc tggcggcgac aggcgcgctg gtcctggcgg cctgcgatgg cggggcgtct
     1441 gcggtt
//
LOCUS       DAOMEO010000947         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8965_length_1445_cov_2.384892, whole genome shotgun sequence.
ACCESSION   DAOMEO010000947 DAOMEO010000000
VERSION     DAOMEO010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8965_length_1445_cov_2.384892"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLH31_17185"
     CDS             complement(<1..528)
                     /locus_tag="PLH31_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQR90964.1"
                     /translation="MNSTKGSVRARLLTSTLLAGLASIATPLAITTIATAVPTLASAQ
                     DYTSGSLSGTVLDANGAPVSGAAVTVKSNAQGFTRSFTTDGSGQFRSSALPAGSYSVS
                     ISKEGFQPTSDGALNVRAGGEAGYNFTLASADAAVSEVVVTATANPQLDFSQTTTGLS
                     IDVEDLQKQVPVGRNV"
     gene            1143..>1445
                     /locus_tag="PLH31_17190"
     CDS             1143..>1445
                     /locus_tag="PLH31_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HQR90965.1"
                     /translation="MSDFRRVTESLFVSPQVTEADMARAAAEGFVLVINNRPDGEDPN
                     QPSSAALEAAARAAGLDYVHIPVVGGPGPSQVEAVQRAADAAGGPVLAFCRSGTRSI"
BASE COUNT          259 a          456 c          462 g          268 t
ORIGIN      
        1 gacgttgcgg ccgaccggaa cttgcttttg caggtcttcg acgtcgatcg acagaccggt
       61 cgtggtttgc gagaagtcga gctgcgggtt ggcggtggcg gtgacgacca cttccgaaac
      121 cgcggcgtca gccgaggcga gcgtgaagtt gtagcctgct tcaccaccgg cgcggacgtt
      181 gagggcgccg tcgctggtcg gctggaagcc ttccttgctg atcgacaccg agtagctgcc
      241 ggccggcagg gccgagctgc ggaactgacc gctgccgtcc gtggtgaacg agcgggtgaa
      301 gccctgggcg ttcgacttca cggtgacggc ggcgccggag accggagcgc cgttggcgtc
      361 aagcacggtg cccgacagcg aaccgctggt gtagtcctga gcgctggcca gggtcggaac
      421 cgccgtggcg atggtcgtga tcgccagcgg cgtcgcgatc gacgcgaggc ctgccagcag
      481 cgtcgacgtc agcagacgcg ctcggacgct acccttcgtg gaattcatgt tcattctatc
      541 ccctctagca tggtgcagcg cgacggccgg actggcccct gaagaaacgt ttgtccggtg
      601 tgcgcggaaa tccgcgccaa gtgcaccacg agattactgg tgtcccggaa aacatttccc
      661 cggttgcggg ggacgttagg agccatgaca gttttgggtc aattgacatt acaacgcggt
      721 aacccacatc gctcaccgct gtcgcataaa cgcaacacgg tcgccccatt ctggtcatag
      781 aggctcggac cattggacgc agccccgtcc gggacgggcc aagatcgccc tagaaccttg
      841 gtttaacggg aaaatttcgc cagcgtaccg tcacggaacg tcgctatctc gacactatgg
      901 attgcggcaa aatccacctc ggcaatcttg acaacgcaga accacattcc aattcatcgc
      961 caaaataacg gtgttttcca ccccttgaac cgatgatttc tgcgtattga tttcagcata
     1021 tgatcgtttc cagggcacat tcaggccgta atgctcgttg ccatggcgcc tgcggctcgt
     1081 gatgatggcc ggcctgtcgc cgcccccgtg gcgagcaggt cagtcagttg agcgagccct
     1141 ccatgagcga cttccggcgc gtcaccgaat ccctcttcgt cagcccgcag gtcacggagg
     1201 ctgacatggc ccgcgctgcg gccgagggat tcgtgctggt gatcaacaat cggccggacg
     1261 gcgaagatcc caaccaaccg tccagcgccg ccctcgaggc ggcggcgcgc gccgccggtc
     1321 tggactatgt ccacattccg gttgtgggcg ggcccgggcc aagccaggtc gaggccgtcc
     1381 agcgcgccgc ggacgccgcc ggcgggccgg tactggcctt ctgtcggtca ggaacccgct
     1441 cgatc
//
LOCUS       DAOMEO010000948         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8966_length_1445_cov_1.923741, whole genome shotgun sequence.
ACCESSION   DAOMEO010000948 DAOMEO010000000
VERSION     DAOMEO010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8966_length_1445_cov_1.923741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLH31_17195"
     CDS             <1..638
                     /locus_tag="PLH31_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HQR90966.1"
                     /translation="AVSPTNAQALDALDAWPRWVDGRLALVGAAGAGKTHLARDWALK
                     SGAAVVEAANPLSAPLDLPALRGRAVLIDDADRRAQGGHLDDETLFHLINMAGVDGGT
                     LLLTGRTAPVAWDCAVADLRSRLNALPVAVIDEPDDIVLEAVLRRALAQRAMTPAEEV
                     YPYLLRRIPRSSLEAIAAVDLLDEAAAAQGRAITRVLAGEVLWGEGDEAED"
     gene            930..>1445
                     /locus_tag="PLH31_17200"
     CDS             930..>1445
                     /locus_tag="PLH31_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HQR90967.1"
                     /translation="MPNTPPLPTPANEADTTCRLDKALLASPERFINRETSWLAFNER
                     VLEESCNPRHPLLERLRFLSISANNLDEFYMVRVAGLKGQVRESVRVVSQDGLTPGEQ
                     LARINASASDLMAEQQKIWREVRAQLWAEGLKLLDAKDIVGDDKVKAEELFLTRMFPV
                     LTPLAIDPAHPF"
BASE COUNT          203 a          499 c          523 g          220 t
ORIGIN      
        1 tcgcggtgtc gccgaccaat gcgcaggcgc tggacgccct ggacgcctgg ccgcgctggg
       61 ttgacgggcg gctggccctg gtgggggctg ccggcgcggg caagacccat ctggcgcgcg
      121 actgggccct gaagtcgggc gctgcggtgg tcgaggccgc caatccgctg tcggcgccgc
      181 tggacctgcc ggccctgcgc ggccgagcgg tgctgatcga cgacgccgac cggcgggcgc
      241 agggcggcca tctggacgac gagaccctgt tccacctgat caacatggcc ggggtcgatg
      301 gcggcacgct gctgctcacc ggccgcacgg cgcccgtggc ctgggactgc gcggtggccg
      361 acctgcgctc gcggctcaac gccctgcccg tggccgtgat cgacgagccc gacgacatcg
      421 tgctggaggc cgtgctgcgg cgggccctcg cccagcgcgc catgaccccg gccgaggagg
      481 tctatcccta cctgctgcgc cgcatccccc gctcgtcgct ggaggccatc gcggcggtcg
      541 acctgctgga cgaggcggcg gcggcccagg gccgggcgat cacccgggtg ctggcggggg
      601 aggtgctgtg gggcgaaggg gatgaggcgg aggactgagg gctgagggct gagggctgag
      661 ggctgagggt cctgctcttc aaaacggctg tcatcccgga caagcgcgca gcgcgcagat
      721 ccgggaccga cgcctgaact cggcgcttcc ggcggtcccg gatctgcgcc cggctttcgc
      781 cggacttgtc cgggatgaca gcttttgttg ctgcggctct caatgtgatc acccgccccc
      841 ttgagcggga gaaggacgct caccctgtct tgcccccgtc acccggcgcg ttcaccatgg
      901 gacaaccccg ttcgcctatg gttccccgca tgcccaatac cccgcccctg cccacgcccg
      961 ccaatgaagc cgacacgacc tgccgcctgg acaaggcgct gctggcctcg cccgagcggt
     1021 tcatcaatcg cgagacctcg tggctggcct tcaatgaacg ggtgctggag gagagctgca
     1081 atccacgcca tccgctgctg gagcgcctgc ggttcctgtc gatctcggct aacaatctcg
     1141 acgaattcta catggtgcgc gtggccggcc tgaaggggca ggtgcgcgag agcgtgcggg
     1201 tggtcagcca ggacggcctg acgccgggcg agcagctggc gcggatcaac gcctcggcct
     1261 ccgacctgat ggccgagcag cagaagatct ggcgcgaggt gcgggcccag ctgtgggccg
     1321 agggcctgaa gctgctcgac gccaaggaca ttgtcggcga cgacaaggtc aaggccgagg
     1381 agctgttcct gacccggatg ttcccggtgc tgacgccgct ggccatcgac ccggcccacc
     1441 cgttt
//
LOCUS       DAOMEO010000949         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8977_length_1444_cov_1.598272, whole genome shotgun sequence.
ACCESSION   DAOMEO010000949 DAOMEO010000000
VERSION     DAOMEO010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8977_length_1444_cov_1.598272"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLH31_17205"
     CDS             complement(<1..1206)
                     /locus_tag="PLH31_17205"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQR90968.1"
                     /translation="MAAAASILAATPSWAQIQITPDQPVSGVVETSDPVAQLGEYSFS
                     HDCYMIDLPEGSSWAVEVTNRDQYLNLSIGRGESCVTLQVDASVASKIFAGRARAALE
                     VTSGGGRYLVRILRPNPFITKSVEYSVALRAATGGKRLPGGEIVQALAQIAPATASTT
                     PTANAGSYADGQVVQDCPTGPQMVVLPAGTLTMGSLATEEARGRDEGPRHAVTFAAAF
                     AVGRTEVTFDQWDGCVAGGGCQRRPADQGWGQGARPVIDVTWNDAMAYAVWLSKATGQ
                     AYHLLSESEWEYAARAGTDTPWNTGEAIVTDDANLLGQFGKTVPVGGYPPNAFGLHDL
                     HGNAAEWVQDCYEDTGYFGAPTDGAAAGLAGCPRRVQRGGSFADEPGRVRSAARKGVA
                     PTQRSPEAGF"
BASE COUNT          251 a          506 c          455 g          232 t
ORIGIN      
        1 gaatccagcc tccggcgagc gctgggtggg cgcgacgccc ttgcgggcgg ccgagcggac
       61 tcgaccgggc tcgtcagcga aggatccgcc acgctggacc cgccttgggc agccggccag
      121 tcccgccgct gcgccatcgg tcggcgcacc gaaatagccg gtatcctcgt agcagtcctg
      181 cacccactcc gcggcattgc catgcaggtc atgaaggcca aaggcgttgg gcggatagcc
      241 gccaaccggt acagtcttgc caaactgacc gagcagattg gcgtcatcgg tgacgatggc
      301 ctcgccggtg ttccagggcg tatccgtccc ggcgcgggcg gcatattccc actcgctctc
      361 agacagtagg tgataggcct gccccgtcgc cttgctcagc cagacggcat aggccatggc
      421 gtcgttccag gtgacgtcga tcacagggcg ggccccctgc ccccagccct gatcggcggg
      481 ccggcgctga cacccccctc ccgcgacaca gccgtcccat tggtcgaagg tgacctcggt
      541 ccgcccaacg gcgaacgctg cggcgaaggt cacggcatgt cggggacctt catctcgacc
      601 gcgcgcctcc tccgttgcca gcgaacccat cgtgagcgtt ccggcaggca gaacgaccat
      661 ctgcggaccg gtggggcaat cctgcacaac ctgtccatcc gcatagctgc cggcattggc
      721 cgttggcgtt gtcgaggctg tcgctggcgc gatctgggcg agcgcctgaa caatctcacc
      781 gccgggaagc cgcttgccgc ccgtggcggc tcgcagcgcg acgctgtact caaccgactt
      841 tgtgatgaac gggttggggc gcaatatccg cacaagataa cggccgccgc ccgacgtcac
      901 ctccaacgcg gctcttgcgc gacccgcgaa gattttcgat gccacgctcg catcaacttg
      961 gagggttacg cagctctctc cacggccaat ggagaggttg agatactggt cgcggttggt
     1021 gacctcgacc gcccagctcg acccctccgg caagtcgatc atgtagcaat catgggaaaa
     1081 ggaatattcg ccgagctgcg cgacgggatc actggtctcg acgacgccag agacgggctg
     1141 atcgggcgtg atctgaatct gggcccaact cggcgtggcc gcaaggatgc tcgcggcggc
     1201 ggccaagccc gaggatcgga cggcgaaccg gatcaacaac ctcgacattt gaaccctcac
     1261 agcgccctcg ccacccggaa accaacctta ggtgatcgtg ccccggccgc cccgcccttt
     1321 cgcgccgccg agcgcagttg cgcgggttca tcggcccagg atccgccgcg ctgaacgcgc
     1381 agggggcagt caggcttcca ggccgccgct cctgtcaacg gcgcaccgaa atagcccatg
     1441 tcgt
//
LOCUS       DAOMEO010000950         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8980_length_1443_cov_2.766571, whole genome shotgun sequence.
ACCESSION   DAOMEO010000950 DAOMEO010000000
VERSION     DAOMEO010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8980_length_1443_cov_2.766571"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="serB"
                     /locus_tag="PLH31_17210"
     CDS             <1..736
                     /gene="serB"
                     /locus_tag="PLH31_17210"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HQR90969.1"
                     /translation="ERDEVEALTALLADLPVDFAIQPVENRRKRLLIADMDSTIINVE
                     CLDELADFAGVKAQVSEITERAMRGELAFEDALRERVGMLTGLSVDALQACYDDRVRL
                     NPGAETLVKTMAAHGARCALVSGGFTFFTSRVAQAAGFHLNRANTLIEADGKLTGKVG
                     DPILGKEAKLAALIEETALLGLTPSDALAVGDGANDLAMIEAAGLGVAYRAKPIVAAQ
                     AHAKVDHADLTALLHFQGYTVSEFAA"
     gene            733..1398
                     /locus_tag="PLH31_17215"
     CDS             733..1398
                     /locus_tag="PLH31_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQR90970.1"
                     /translation="MSFPRRVEAVVFDMDGLLLDTEIVYRAAMIEAGSVFGVAFTGEI
                     YASMVGRTNPECGVLLRDLYGEAFPVQAYFERVWSDVEDLLEAETKLKTGVIEILDYL
                     DEVGLPCAIATSNGMVSVEKYLGRFDLLSRFNAVVANADVIRHKPHPDPYLEAASRLG
                     VDPAHCLALEDSHPGVRAAHAAGMMTVMVPDILDPNEEMHDKCVHIADSLHVVLDLLK
                     ASV"
BASE COUNT          230 a          481 c          471 g          261 t
ORIGIN      
        1 cgagcgcgat gaggtcgagg cgctgacggc gctgctggcc gacctgccgg tcgatttcgc
       61 gatccagccg gtcgagaacc gcaggaagcg cctgctgatc gccgacatgg actccaccat
      121 catcaatgtc gaatgcctgg acgaactggc tgatttcgct ggcgtgaaag cgcaggtctc
      181 cgaaatcacc gagcgggcca tgcgcggcga actggccttt gaggacgccc tgcgcgagcg
      241 ggtcggtatg ctgaccggcc tgtcggtcga cgccctgcag gcctgctacg acgaccgcgt
      301 gcggctcaac cccggcgccg agaccctggt gaagaccatg gcggcgcatg gcgcgcgctg
      361 cgccctggtc tcaggcggct tcaccttctt caccagccgg gttgcgcagg cggcgggctt
      421 tcatctgaac cgcgccaaca ccttgatcga ggccgatggc aaactgaccg gcaaggtcgg
      481 tgatccgatc ctgggcaagg aggccaagct ggccgccctg atcgaggaga ccgcgctgct
      541 gggcctgacc cccagcgacg ccctggccgt gggcgacggc gccaacgacc tggccatgat
      601 cgaggccgcc ggcctgggcg tcgcctatcg cgccaagccc attgttgcgg cccaggccca
      661 cgccaaggtc gaccatgccg acctcaccgc cttgcttcac ttccagggct atacggtctc
      721 ggagtttgca gcatgagttt cccccgccgc gtcgaagccg tcgtcttcga catggatggc
      781 ctgttgctgg acaccgagat cgtctatcgc gccgccatga tcgaggccgg ctcggtgttt
      841 ggcgtcgctt tcaccggcga gatctatgcc tcgatggtcg gccggaccaa tccggaatgc
      901 ggcgtgctgc tgcgcgatct ctatggcgag gcgtttccgg tccaggccta tttcgagcgt
      961 gtctggagcg acgtcgagga cctgctggag gccgagacca agcttaagac cggcgtgatc
     1021 gagatcctcg actatctgga cgaggtcggc ctgccgtgcg ccatcgccac ctcgaacggc
     1081 atggtgtcgg tcgagaagta tctggggcgc tttgatctgc tgagccgctt caacgcggtg
     1141 gtcgccaatg ccgacgtcat tcgccacaag ccgcatcccg atccctatct ggaggccgcc
     1201 agccgcctgg gcgtcgatcc cgcccactgt ctggcgctgg aggattcaca ccccggcgtc
     1261 cgcgccgccc atgcggccgg catgatgacg gtgatggtgc ccgacatcct cgatcccaac
     1321 gaggagatgc acgacaagtg cgtccacatc gccgacagcc tgcatgtggt gctggatctg
     1381 cttaaggcgt cggtctgagt tgagcggctt caggcctggc gcagcgcggc cgtctctgtc
     1441 tcg
//
LOCUS       DAOMEO010000951         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_8986_length_1443_cov_1.968300, whole genome shotgun sequence.
ACCESSION   DAOMEO010000951 DAOMEO010000000
VERSION     DAOMEO010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8986_length_1443_cov_1.968300"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PLH31_17220"
     CDS             <1..1156
                     /locus_tag="PLH31_17220"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQR90971.1"
                     /translation="GKTALVRVDFNVPVEGGKITDDTRLRSALPTIQYLTGKGAKVAL
                     LAHFDRPKGKVVPQMSLAFVAEPLAALLGAPVAFAGDCIGDEAAKVIAALPAGGVALL
                     ENVRFHAGEEKNDAAFAADLAKLGDLYVNDAFSAAHRAHASTEALARALPAYPGLAMQ
                     RELEALDSALGNPQKPVIGIVGGSKVSTKLDLLNNLVAKLDRLAIGGGMANTFLHAQG
                     HDIGGSLCEKDLADTAREIIEKAKAAGCELLLPVDVVVAKKVAPGVDAETRSLADVQA
                     DDLILDAGPETTKRLLAAMDASKTLIWNGPLGVFEVPPFDEATVLAAKHAAQLAKSGK
                     IVAVAGGGDTVSALNHAGVVGDMTFVSTAGGAFLEWMEGKDLPGVSALNT"
     gene            1294..>1443
                     /locus_tag="PLH31_17225"
     CDS             1294..>1443
                     /locus_tag="PLH31_17225"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQR90972.1"
                     /translation="MARITLRQLLDHAAEHEYGLPAFNINNMEQGLAIMEAADAVNSP
                     VIIQAS"
BASE COUNT          265 a          490 c          465 g          223 t
ORIGIN      
        1 cggcaagacc gccctggtcc gcgtggactt caacgtgccc gtcgagggcg gcaagatcac
       61 tgacgacacc cgcctgcgct cggccctgcc gaccatccag tatctgaccg gcaagggcgc
      121 caaggtggcg ctgctggccc acttcgaccg acccaagggc aaggtggttc cccagatgag
      181 cctggccttc gtggccgagc cgctcgccgc cctgctgggc gccccggtgg ccttcgccgg
      241 cgactgcatc ggcgatgagg ccgccaaggt gatcgccgcc ctgccggccg gcggcgtcgc
      301 cctgctggaa aacgtccgct tccacgccgg cgaagagaag aacgacgcgg ccttcgccgc
      361 cgacctcgcc aagctgggcg acctctatgt caacgacgcc ttcagcgccg cccaccgcgc
      421 tcacgcctcg accgaagccc tggcccgcgc cctgccggcc tatccgggtc tggccatgca
      481 gcgcgagctg gaggccctcg actcggccct gggcaatccg cagaagccgg tgatcggcat
      541 cgtcggcggc tccaaggtct cgaccaagct ggatttgctc aacaacctgg tcgccaagct
      601 ggatcgcctg gcgatcggcg gcggcatggc caacaccttc ctgcacgctc agggccacga
      661 tatcggcggc tcgctctgcg aaaaggacct ggccgacacc gcccgcgaga tcatcgaaaa
      721 ggccaaggct gccggctgcg aactgctgct gcccgtcgac gtggtggtgg ccaagaaggt
      781 cgcgcccggc gtcgacgccg agacccgcag ccttgctgac gtgcaagccg acgacctgat
      841 cctcgacgct ggtcccgaaa cgaccaagcg cctgctggcc gccatggacg ccagcaagac
      901 cttgatctgg aacggccctt tgggcgtttt tgaagtccct ccgttcgacg aagcgaccgt
      961 tttggcggcg aaacatgccg ctcagctggc aaaatcgggt aagatcgtcg ccgtggcggg
     1021 cggcggtgat acggtgtcgg cgctgaatca cgcgggcgtc gtgggcgata tgaccttcgt
     1081 ctcgaccgcc ggcggcgcgt ttcttgagtg gatggagggc aaggacctgc ccggtgtctc
     1141 ggctttgaac acctaaggga cccagatttt caggcgcgga cctccgggag gggtccgcgt
     1201 gagctacgga cgaccgggca gagaacgcaa aagcgtcacg ccccaaaaac aagaaacgac
     1261 ccaaaccttt caagcttgca ggagagaata cacgtggcga ggatcacgct taggcagttg
     1321 ctggaccatg ccgcagagca tgagtacggg ctgccggcct tcaatatcaa caacatggaa
     1381 cagggtctgg cgatcatgga ggcggccgac gccgtcaact cgccggtgat catccaggcc
     1441 tcg
//
LOCUS       DAOMEO010000952         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9000_length_1441_cov_2.717893, whole genome shotgun sequence.
ACCESSION   DAOMEO010000952 DAOMEO010000000
VERSION     DAOMEO010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9000_length_1441_cov_2.717893"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1144)
                     /locus_tag="PLH31_17230"
     CDS             complement(281..1144)
                     /locus_tag="PLH31_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90973.1"
                     /translation="MPAAASADALGDYVAARDTAIAASVAAAKAGKSGDAAVVTREQA
                     AFMDLGKRLATALGPLKFKGLGPPIYTLLTFTYDESSPTHLLDGLAFANKDVSTRLVV
                     TPDSVFQPWLAARAKDDGAPAALAGGVKTAMTTAEFFQDALAFDGGYFQPYVDLPVAA
                     APGETALAVLGLQADEPPGNIAPNQIAIVRIADGKAMVGLTMVKLDIKPIAACEAIWK
                     PYKTKVEALLKAIEKDDKSEDPRWEELTRINDAGSQAFRVCFAREAPAQPFFPVAVKR
                     ADALLDIARGK"
BASE COUNT          225 a          479 c          504 g          233 t
ORIGIN      
        1 cgcaataagc ctctacacct gcagtcctca gcggtggcgc cggaaacggc gacttggaga
       61 acggacggat gtactccggc tccagagcat agctcgagac aggaagcccc gcgtgagcgg
      121 ggcttttttg ttatctatac gcgctctaaa tcccttcctc tagggcatct aggacgctca
      181 agcgtcgtga gaatgacggc cgcccgaggt tcgcgggccg accaaccggt cacgcccgcc
      241 cgccggggac gacaagtcta ctcaagttta ctaatgcccc tcacttgccg cgggcgatgt
      301 cgagcagggc gtccgcgcgc ttgaccgcca cggggaagaa cggctgcgcc ggcgcctccc
      361 tggcgaagca gacgcggaag gcctgcgagc ccgcgtcatt gatgcgggtc agctcctccc
      421 agcgcggatc ctcgctctta tcgtcctttt cgatcgcctt cagcagggcc tcgaccttgg
      481 tcttataggg cttccagatc gcctcgcagg ccgcgatggg cttgatgtcc agcttcacca
      541 tggtcaggcc gaccatggcc ttgccgtcgg cgatccggac gatggcgatc tggttcgggg
      601 cgatattgcc gggcggctcg tcggcctgga ggccgagcac cgcgagcgcg gtttccccgg
      661 gcgccgctgc caccggcagg tccacatagg gctggaaata gccgccgtcg aaggcgagcg
      721 catcctggaa gaattcagcg gtggtcatgg ccgtcttcac gccgccggcg agcgcggcgg
      781 gtgccccgtc gtccttggcg cgcgcggcca gccagggctg gaacacgctg tccggcgtga
      841 cgacgaggcg ggtgctgaca tccttgttcg cgaaggccag cccgtcgagc aggtgggtgg
      901 ggctgctctc gtcataggtg aaggttaaga gcgtgtagat cggtgggccc agccccttga
      961 acttgagcgg gccgagggcg gtcgccagcc gcttgccgag atccatgaag gcggcctgct
     1021 cgcgcgtgac caccgccgca tccccgctct tgcccgcctt ggcggcggcg accgatgcgg
     1081 cgatcgccgt gtcgcgggcg gcgacataat cccccagcgc gtcggcggag gcggcggcgg
     1141 gcaacgcaat ggcgaggacc agggagctgg cgaaggcgag ggcgtgaacg aagcttctca
     1201 tggagcttcc cttgaaaggt cgaggccgca gggcggggga agcgctaccg tcaggtcagt
     1261 catggccagg gtcaagctgg aaatccgcgc ctgcgccatc ttgccgtccc caggggcatc
     1321 cctgcgccgc atccccgaga ctcgccccgg ctattccgcc ggcgccagcg gcggggcggc
     1381 gccgcctgtc tcctcgcgcg cgggcgccgg ccggcgccgc aggcggtcga gcagcagata
     1441 g
//
LOCUS       DAOMEO010000953         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9001_length_1441_cov_2.485570, whole genome shotgun sequence.
ACCESSION   DAOMEO010000953 DAOMEO010000000
VERSION     DAOMEO010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_1441_cov_2.485570"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>225
                     /locus_tag="PLH31_17235"
     CDS             <1..>225
                     /locus_tag="PLH31_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HQR90974.1"
                     /translation="RGPTGVAVVVSTGDAEDVESVKREVERVSQVLGRTLTYLVGKPG
                     LDGHSNGAEQIATRARACGMEVVYDGIRSTP"
     assembly_gap    226..235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <236..519
                     /locus_tag="PLH31_17240"
     CDS             <236..519
                     /locus_tag="PLH31_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518550.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQR90975.1"
                     /translation="EIVRRAKESRAHVVGLSILSGSHLDLVQEVVRVMRVEGLGDIPV
                     VVGGIIPDADALILKQMGVARIYTPKDFKITDIMGDIVKLVEGAALVEA"
     gene            570..854
                     /locus_tag="PLH31_17245"
     CDS             570..854
                     /locus_tag="PLH31_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HQR90976.1"
                     /translation="MKAELLLQERHQVAEAAFATIKIWRVAEPVRGSQHDLKYSLAYV
                     VSGVCVLRYDNEAGKGDHRHLGELEMPYVFSTPQALLADFWNDVDRWRPQ"
     gene            851..1195
                     /locus_tag="PLH31_17250"
     CDS             851..1195
                     /locus_tag="PLH31_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQR90977.1"
                     /translation="MTTVTLSVASRDDLNRRVQSAFAGEAQGAHISFASAELLWTTLT
                     RKRWEILRAMTGQGPLSIREVARRVERDVKAVHGDIHALLDAGVLEAAPGGGVAFPYD
                     AVHVDFMLTAAA"
     gene            1218..>1441
                     /locus_tag="PLH31_17255"
     CDS             1218..>1441
                     /locus_tag="PLH31_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR90978.1"
                     /translation="MTLDDALARLRAAKPLLDHYGVQRVGVFGSTARGEAGPDSDVDV
                     LVEFAPKVLPGFGVFRLERDLAALFERPVDL"
BASE COUNT          225 a          448 c          497 g          261 t
ORIGIN      
        1 cgaggcccga ccggcgtggc cgtcgtggtc tcgaccggtg acgccgagga cgtcgagtcc
       61 gtcaagcgcg aggtcgaacg ggtctcgcaa gtgctgggcc gcaccctgac ctatctggtc
      121 ggcaagccgg gcctggatgg tcactccaac ggcgccgagc agatcgccac ccgggcccgg
      181 gcctgcggca tggaggtcgt ctatgacggc atccgctcga ccccgnnnnn nnnnnacgag
      241 atcgtccgcc gcgccaagga gagccgggcc catgtggtgg ggctgtcgat cctgtcgggc
      301 tcgcacctgg atctggtgca ggaagtggtg cgtgtgatgc gcgtcgaggg gctgggcgac
      361 atccccgtcg tggtgggcgg catcatccct gacgccgacg ccctgatcct gaaacagatg
      421 ggtgtggcgc ggatctacac ccccaaggac ttcaagatca ccgacatcat gggcgacatc
      481 gtcaagctgg tggaaggcgc ggcgctggtc gaggcttgag ttgtttgagt atttgacaac
      541 atgcccggcc tgttggtaaa aagtccaaca tgaaggctga gcttctcctg caggagcgcc
      601 accaggtcgc ggaggccgct ttcgcgacga tcaagatctg gagggtcgct gagccggtgc
      661 ggggctccca gcacgatttg aagtattcgc tggcctatgt ggtgagcggc gtttgcgtgc
      721 ttcgctatga caacgaggct ggcaaaggcg atcaccggca cctgggtgag cttgaaatgc
      781 cctacgtgtt cagcacgccg caggccttgc tcgctgactt ctggaacgat gtcgaccgat
      841 ggaggcccca atgaccactg tgaccctgtc cgtcgcatct cgggacgatc tgaaccggcg
      901 cgtccagtcg gccttcgccg gcgaggccca gggcgcacac atctcgttcg cctcggccga
      961 actgctctgg accaccttga cccgcaagcg ttgggagatc ctgcgcgcca tgacggggca
     1021 ggggcccctg tcgatccgcg aggtcgcccg ccgggtcgag cgcgatgtga aggccgtgca
     1081 tggcgacatc cacgccctgc tcgacgccgg cgtgctggaa gctgcgcccg gcggcggggt
     1141 ggctttcccc tacgacgccg tccatgtcga cttcatgctg acggcggcgg cctgagtttc
     1201 atgctataat tcaggtcatg accctcgacg acgccctcgc cagactgcgc gcggccaagc
     1261 cgctgctgga ccactatggc gtccagcgcg tgggcgtgtt cggctcgacg gcccggggtg
     1321 aggcgggccc ggacagcgac gtggacgtgc tggtggagtt cgcaccgaag gtgctgccag
     1381 ggtttggcgt cttccggctt gagcgtgatc tggccgccct gtttgagcgg ccggtcgatc
     1441 t
//
LOCUS       DAOMEO010000954         1441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9002_length_1441_cov_2.183983, whole genome shotgun sequence.
ACCESSION   DAOMEO010000954 DAOMEO010000000
VERSION     DAOMEO010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9002_length_1441_cov_2.183983"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PLH31_17260"
     CDS             <1..100
                     /locus_tag="PLH31_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR90979.1"
                     /translation="EEEFDIEIPDDAAEHIQTVGDAVKFITEKTGA"
     gene            273..>1441
                     /gene="fabF"
                     /locus_tag="PLH31_17265"
     CDS             273..>1441
                     /gene="fabF"
                     /locus_tag="PLH31_17265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQR90980.1"
                     /translation="MRRVVVTGLGLLTPLGQGVEVSWKNILAGKSGANRISAFDPTDY
                     ACKVACEVPRVDGRGGGGPDVEGAFNPDLTMSPRDQKRVDDFILYGIAAADEAVKDSG
                     WVPETEEDRYRTGVILGSGIGGLSTIADTALELEAKGPRKVSPFFISSALINLISGQV
                     SIRYGFKGPNHAVVTACATGAHAIGDAARLIKYGDADVMVAGGAEAAVCKVGIAGFIA
                     CRAVSTDFNDTPEKASRPYDQDRDGFVMGEGAGALVLEEYEHAKARGAKIYAEVVGYG
                     LSGDAYHITAPSEDGDGGFRALSAAVKDAGLQPSDIDYVNAHGTSTMADGIEAKAVER
                     FLGNHAANVVMSSTKSMTGHLLGAAGAIEAIFSILAIRDQIAPPTINLDNPNVEVA"
BASE COUNT          250 a          497 c          466 g          228 t
ORIGIN      
        1 cgaggaagaa ttcgacatcg aaatcccgga cgacgccgcc gagcacatcc agacggttgg
       61 cgacgccgtg aagttcatca cggaaaagac cggcgcctaa gggcgccagg ttcgcctctg
      121 aggcgaacgg actatcaccg ccgcgcagca cgggctcttg ccatcgtgcg cgcggcggtt
      181 ttcgtatgtg gccgagagtc gcggcctggg gaggcgtcgg attcgcccct tcatccccat
      241 atgcccacga cgacgaaggt taggagtgat ccatgcgtag agttgttgtc accggactgg
      301 gcctgctgac cccgctgggt cagggcgtcg aagtgtcctg gaagaacatc ctcgccggca
      361 agtcgggcgc gaaccgcatc tcggccttcg atcccaccga ctacgcctgc aaggtggcct
      421 gcgaggttcc gcgcgtcgat gggcgcggtg gcggcggccc tgacgttgaa ggcgccttca
      481 accccgacct gaccatgagc ccgcgcgacc agaagcgcgt ggacgacttc atcctgtacg
      541 gcatcgccgc cgcagacgag gccgtgaagg attccggctg ggttcccgag accgaggaag
      601 accgctaccg caccggcgtg atcctcggat cgggtatcgg cggcctgtcg accatcgccg
      661 acaccgcgct cgagctcgag gccaagggcc cgcgcaaggt cagccccttc ttcatctcct
      721 cggccctgat caacctgatc tcgggccagg tttcgatccg ctacggcttc aagggcccca
      781 accacgccgt agtcacggcc tgcgccacgg gcgcccacgc catcggtgac gccgctcgcc
      841 tgatcaagta cggcgacgcc gacgtgatgg tggccggcgg cgccgaggcg gcggtttgca
      901 aggtcggcat cgccggcttc atcgcctgcc gcgccgtctc gaccgacttc aacgacacgc
      961 ctgaaaaggc ttcgcgtcct tacgaccagg accgcgacgg cttcgtgatg ggcgagggcg
     1021 ctggcgccct ggtgctggaa gagtacgagc acgccaaggc ccgcggcgcc aagatctatg
     1081 ccgaggtcgt gggctacggc ctgtccggtg acgcctatca catcactgcc ccctccgagg
     1141 acggcgacgg cggcttccgg gccctgtcgg cggccgtgaa ggacgctggt ctgcagccgt
     1201 cggacatcga ctacgtcaac gcccacggca cctcgaccat ggccgacggc atcgaagcca
     1261 aggccgtcga gcggttcctg ggcaatcatg ccgccaacgt ggtgatgtcg tcgaccaagt
     1321 cgatgaccgg acacctgctg ggcgcggccg gcgccatcga ggccatcttc tcgatcctgg
     1381 ccatccgcga ccagatcgcg ccgccgacca tcaacctgga taaccccaat gtggaggtcg
     1441 c
//
LOCUS       DAOMEO010000955         1440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9012_length_1440_cov_2.879422, whole genome shotgun sequence.
ACCESSION   DAOMEO010000955 DAOMEO010000000
VERSION     DAOMEO010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_1440_cov_2.879422"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /gene="ccrM"
                     /locus_tag="PLH31_17270"
     CDS             complement(<1..530)
                     /gene="ccrM"
                     /locus_tag="PLH31_17270"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase CcrM"
                     /protein_id="HQR90981.1"
                     /translation="MTFGPETIIQGDCIEAMNALPEKSVDLIFADPPYNLQLGGDLLR
                     PDNSKVDAVDDHWDQFESFAAYDKFTREWLKAARRVLKDDGAIWVIGSYHNIFRVGVA
                     VQDLGFWILNDVIWRKSNPMPNFKGTRFANAHETLIWASKSQAAKRYTFNYDALKMAN
                     DEVQMRSDWTIPLCTG"
     gene            complement(669..1316)
                     /locus_tag="PLH31_17275"
     CDS             complement(669..1316)
                     /locus_tag="PLH31_17275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQR90982.1"
                     /translation="MPPGPDMMLERACGPGPVCGVDEAGRGPWAGPVSAGAVILDPAR
                     IPRGLNDSKKLSAKARAALEIEIKETAIAWGVGLASIEEIEELNILHAAGLAMRRAIE
                     ALPVTPAFALVDGNYAFKLPCPIRTVIKGDSLSCSIAAASILAKEARDRIMVEMDGLY
                     PGYGFAGHKGYHAPIHVEGLRRLGPSPIHRMSWAPVKAALAGDLIRDFAGENLEA"
BASE COUNT          232 a          450 c          484 g          274 t
ORIGIN      
        1 tcgccggtgc aaagcgggat ggtccagtcc gagcgcatct gcacctcgtc attggccatc
       61 ttcagggcgt cgtagttgaa ggtgtagcgc ttggccgcct ggctcttgga ggcccagatc
      121 agggtctcgt gggcgttggc gaagcgggtg cccttgaagt tgggcatcgg gttggacttg
      181 cgccagatca cgtcgttgag gatccagaag cccaggtcct gcacggccac gccgacgcgg
      241 aagatgttgt gatagctgcc gatcacccag atcgcgccgt cgtccttcag cacccggcgg
      301 gcggctttca gccactcgcg ggtgaacttg tcgtaggcgg cgaagctctc gaactggtcc
      361 cagtggtcgt cgaccgcgtc gaccttggaa ttgtccggac gcagcaggtc gccgcccagc
      421 tgcagattgt agggcggatc ggcgaagatc aggtcgaccg acttctcggg cagggcgttc
      481 atcgcctcga tgcagtcgcc ttgaatgatg gtctctggcc cgaaggtcat gtccgcttcc
      541 tgtaaaacag tcagcggaat catgagtctt cctggtaaac gggtcgtgtc gaagcgtggt
      601 tagcgggccg ttaaccccgc ccagaagcgt cgatttgagt ctgttcagac tcataatcgt
      661 cagcgcaatc aggcctctag gttttcgccg gcgaaatcgc ggatcaggtc gcccgccagc
      721 gccgccttga ccggcgccca gctcatccgg tggatcggcg aggggcccag cctgcgcaga
      781 ccctccacgt ggatgggcgc gtgatagccc ttgtggccgg caaagccgta gccgggatag
      841 aggccgtcca tttccaccat gatccggtcg cgggcttcct tggccaggat cgaggccgcg
      901 gcgatcgagc acgacaggct gtcgcccttg atcaccgtcc tgattgggca gggcagcttg
      961 aaggcgtaat tgccgtccac cagggcgaag gccggcgtca ccggcagggc ctcgatggcc
     1021 cggcgcatgg ccaggccggc ggcgtgcagg atgttgagtt cctcgatctc ttcgatgctg
     1081 gccaggccga cgccccaggc gatggccgtt tccttgatct cgatctccag ggccgcgcgg
     1141 gccttcgccg acagcttctt ggagtcgttg aggcccctgg gaatgcgggc cggatccagg
     1201 atcacggcgc cggcgctgac cggcccggcc caagggccac ggccggcctc gtccaccccg
     1261 cagaccgggc cgggtccgca ggcccgttcc agcatcatgt cgggaccggg aggcatgggt
     1321 cgatgcatag gcagatttgc cgggtccttc aaactgtcat cccggccaag tgcgcaacgc
     1381 gccgagccgg cgactgtgtt ggatcaagcg ggtttttgcc aaggctgtcc cgacttgagc
//
LOCUS       DAOMEO010000956         1438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9030_length_1438_cov_2.039769, whole genome shotgun sequence.
ACCESSION   DAOMEO010000956 DAOMEO010000000
VERSION     DAOMEO010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9030_length_1438_cov_2.039769"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>600)
                     /locus_tag="PLH31_17280"
     CDS             complement(<1..>600)
                     /locus_tag="PLH31_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518582.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HQR90983.1"
                     /translation="RQGRDLADAFGEAVGEGLAAAQLDPEEIETGHVGNFAGELFAGQ
                     GLLGGLFGQVHPAFDGLPTARHEAACASGSVAILAATAEIEAGRYGLACVVGIELMRN
                     VPGQKAAENLGSAAWAGHEFQDARFLWPAAFSDLAEEYDRRYGLKYEHLMGISEINFA
                     NGRRNPNAQTRDWRFGPEAFTENDETNPVVEGRTRKQDCG"
     assembly_gap    601..610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            749..>1438
                     /locus_tag="PLH31_17285"
     CDS             749..>1438
                     /locus_tag="PLH31_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HQR90984.1"
                     /translation="MPQPIKAPTLVRNSSAARALNLIGDRWTLLLLYAAFMGVKRFDG
                     FIALTGMARSLLVDRLGRLEAAGVLEKRPYQTRPPRHEYHLTAMGRDLYDSALMLLGW
                     EMRWRHDPDCPSHQIIHSPCGQALRPVLVCQACAAPVKVRDITLTPGPGAGLEPAPRA
                     RHSRRASDAQSAEGVGGVPLHPMLERSIEVLGDRWTAHLVAASFYGLTRFKDLQAELK
                     VASNILTDRLSR"
BASE COUNT          217 a          526 c          459 g          226 t
ORIGIN      
        1 accgcagtcc tgcttgcggg tgcggccttc gaccaccgga ttggtctcgt cgttctcggt
       61 gaaggcctcg ggcccgaacc tccagtcgcg ggtctgggcg ttgggattgc ggcggccgtt
      121 ggcgaagttg atctccgaga tccccatcag gtgctcgtat ttcagaccgt agcggcggtc
      181 atattcctcg gccagatcgg agaaggcggc gggccacagg aaccgtgcgt cctggaattc
      241 atgccccgcc caggcggcgc tgcccaggtt ctcggccgcc ttctggccgg gcacgttgcg
      301 catcagctcg atgccgacca cgcaggccag gccgtagcgg ccggcctcga tctcggcggt
      361 agcggccagg atggcgacgc tgcccgaggc gcaggcggcc tcatggcggg ccgtcggcag
      421 gccgtcgaag gccgggtgga cctggccgaa caggccgccc agcaggccct gcccggcaaa
      481 cagctcgccc gcgaaattgc cgacgtggcc ggtctcgatc tcttcaggat ccagttgcgc
      541 cgccgccagc ccttcaccga cagcctcgcc aaaggcgtcg gccaagtcgc gaccctgacg
      601 nnnnnnnnnn agtcgctctg ccatccaccc agcacataga ccaaggccgc ctccctcgcc
      661 gctcgacagt gagtccgact atacaagtga ctgatccgga gtccatgccc ggcgttgacg
      721 cccgccgcgc ggcgccgcac caagccccat gcctcagccg atcaaggccc cgaccctcgt
      781 ccgcaacagc tcggccgccc gcgcgctgaa cctgatcggc gaccgctgga ccctgctgct
      841 gctctatgcg gccttcatgg gcgtcaagcg cttcgacggc ttcatcgccc tgacgggcat
      901 ggcgcgctcg ctgctggtcg accgcctggg ccggctggag gcggccggcg tgctggaaaa
      961 gcgcccctat cagacccgcc ccccgcgcca cgagtaccat ctgaccgcca tgggcaggga
     1021 tctctacgac tcggccctga tgctgctggg ctgggagatg cgctggcggc acgacccaga
     1081 ttgcccctcg caccaaatta tccacagccc ctgtggacaa gctctgaggc ccgttctggt
     1141 ctgtcaggcc tgcgccgcgc cggtaaaggt gcgcgacatc accctgacgc ccggtccggg
     1201 cgctggcctg gaacccgcgc cccgcgcccg acattcgcgc cgggccagcg acgcccagag
     1261 cgcagaaggc gtgggcggcg tccctctgca tccgatgctg gagcgctcca tcgaggttct
     1321 gggcgaccgc tggaccgccc acctggtcgc ggccagcttc tacggcctga cgcgattcaa
     1381 ggatctgcag gccgagttga aggtggccag caacatcctc actgaccggc tgtcacgt
//
LOCUS       DAOMEO010000957         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9039_length_1437_cov_2.105644, whole genome shotgun sequence.
ACCESSION   DAOMEO010000957 DAOMEO010000000
VERSION     DAOMEO010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9039_length_1437_cov_2.105644"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="PLH31_17290"
     CDS             <1..>1046
                     /locus_tag="PLH31_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR90985.1"
                     /translation="QVLADPARLQALGLSIEELARTLSSNNVVVATGRLEDRYRLYLV
                     LADNRLTGIEDIRRTVVQTGSRGVVELDDIAQVVTRQAPQWTRVTSQGRDAVLLNIRQ
                     TPDANSVALVKEVRKRIAGFASQTPPDVQIVTYYDQSQLVTAAASSVRDSILLGALLA
                     GIVLFVFLRSLRFMLIVALLLPATLAATTIVLTLMGMSFNMMTLGGMAAAVGLVVDDA
                     VVMLEHLMRRLQERHRDSAAGGSVLEAASEMIRPLIGSTLATVLVFAPLAFLGGVTGG
                     FFKALAITMSAALVISLLFALFVAPLLADRWVRDRDVQQAEKSGAILDRFGGRYEMLM
                     RHLLKAPGRIALVV"
     assembly_gap    1047..1056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1057..>1437
                     /locus_tag="PLH31_17295"
     CDS             <1057..>1437
                     /locus_tag="PLH31_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR90986.1"
                     /translation="YAAPGTSLAETDRLLRQVEVILQNTPDVESYSRRTGLQLGGSLS
                     EANEGDFFVRLKAKGRRPIEAVTADVRSRVEATVPGLTISTAQLMEDLIGDLTAVPQP
                     IEVKLLGADETALKAASPQVVEAI"
BASE COUNT          229 a          474 c          460 g          264 t
ORIGIN      
        1 accaggtcct ggccgacccg gcccgcctcc aggcgctggg cctctctatc gaagagctcg
       61 ctcgcaccct gtcgtccaac aatgtggtgg tcgccacggg ccggctggaa gaccgctatc
      121 gcctgtatct ggtcctggcg gacaaccgcc tgaccggcat cgaagacatc cgccgcaccg
      181 tggtccagac cggcagccgc ggcgtggtgg aactggacga tatcgctcag gtcgtgacgc
      241 gtcaggcccc gcagtggacc cgggtgactt cgcaaggccg cgacgcggtt ttgctgaaca
      301 tccggcagac ccccgacgcc aacagcgtcg ccctggtcaa ggaggtgcgc aaacggatcg
      361 ccgggttcgc atcccagacc ccgccggacg tgcagatcgt cacctactac gatcagtccc
      421 agttggtcac cgcagccgcc agcagcgtgc gggactccat acttctgggc gccctgctgg
      481 ccgggatcgt gttgttcgtc tttctgcgca gcttgcgctt catgctgatc gtcgcccttc
      541 tgctgccagc gacgctggcg gccacaacca tcgtgctcac cctgatgggg atgagtttca
      601 acatgatgac cttgggcggc atggcggcgg ccgtcggcct cgtcgtcgat gatgcggtcg
      661 tcatgctcga acatctgatg cgacggctac aggaacgcca tcgcgattca gccgccggcg
      721 ggagcgtcct ggaggccgcg tccgaaatga tccgccccct gatcggatcg acacttgcga
      781 cggtgctcgt cttcgccccg ttggccttcc tcggcggcgt caccggcggc tttttcaagg
      841 cgctggcgat caccatgtcg gccgccttgg tcatttctct tctgttcgcc ctgttcgtcg
      901 caccgctcct agcagaccgc tgggttcggg accgcgacgt acaacaggcc gaaaaaagcg
      961 gcgcgatcct ggatcggttc ggcgggcgct atgagatgtt gatgcgccac ctgctcaagg
     1021 ctccgggtcg gatcgccttg gttgttnnnn nnnnnnacta tgcggcgccg gggacctctc
     1081 tggccgagac cgaccggttg ctgaggcagg tcgaggtcat tctacagaac acgcccgacg
     1141 tcgaaagcta ttcgcgccgg acgggcctgc agctcggcgg cagtctgtcc gaggccaatg
     1201 agggcgactt cttcgtgcgc ctgaaggcaa agggccggcg gcccatcgag gcggtcacag
     1261 cggatgtccg cagtcgcgtg gaagcgaccg tgccgggtct gacgatctcc acggcgcagc
     1321 tcatggagga cctgatcggc gacctgaccg ccgtgccgca accgatcgaa gtcaagctgt
     1381 tgggcgcgga cgaaacggct ttgaaggccg cctcgcctca ggtcgtggaa gccatcg
//
LOCUS       DAOMEO010000958         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9076_length_1431_cov_2.853198, whole genome shotgun sequence.
ACCESSION   DAOMEO010000958 DAOMEO010000000
VERSION     DAOMEO010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9076_length_1431_cov_2.853198"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLH31_17300"
     CDS             <1..712
                     /locus_tag="PLH31_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR90987.1"
                     /translation="GLAAIPLAVAFVVYMLMAKDAPEQPQPKKLTEYLDVLKAPDAWW
                     LMLLYAVTFGGFVGLASSLTMYFNSEYGLDPVIAGFFTAACVFAGSFIRPVGGALADR
                     HGGVRTLTVVYALAAIGMAVASFQLPSAWLALAVLMFSMLALGAGNGAVFQLAPQRFR
                     KEIGVMTGLIGMTGGIGGFYLASTLGMAKKATGSYQLGFLGFAALAVFALFALHSLKA
                     RWRAVWPTLHADARPVRV"
     gene            741..>1431
                     /locus_tag="PLH31_17305"
     CDS             741..>1431
                     /locus_tag="PLH31_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR90988.1"
                     /translation="MSKAPTRERLIVIGNGMAGCRAVEEILKRDPDRYEIAIFGTEPR
                     VNYNRIMLSPVLAGEKTFDDIVINDEAWYRDNGIALHVGRAVIGVDLEGKTVLAEGGL
                     ALAYDKLILATGSDPFRLPLPGSDLKGVVTFRDLDDVEVMVAASAKPGARAVIIGGGL
                     LGLEAAYGLARRGMGATVVHLMDVLMERQLDESAGFLLREALLERGVETVMGAHSEEI
                     IGENGQVTGLKL"
BASE COUNT          213 a          472 c          476 g          270 t
ORIGIN      
        1 tggcctggcc gccatcccgc tggccgtcgc cttcgtcgtc tacatgctga tggccaagga
       61 cgcgcccgag cagccgcagc ccaagaagct gaccgagtat ctcgacgtat tgaaggcgcc
      121 cgacgcctgg tggctgatgc tgctgtacgc cgtaaccttc ggcgggttcg tgggtctggc
      181 ctcgtcgctg accatgtact tcaactccga atatggcctg gacccggtca tcgccggctt
      241 cttcaccgcc gcctgcgtat tcgccggatc gttcatccgt cccgtcggcg gggccctggc
      301 cgaccgccat ggcggcgtac gcaccctgac cgtggtctac gccctggccg ccatcggcat
      361 ggccgtggcc agtttccagt tgccttccgc ctggctggct ttggcggtgc tgatgttctc
      421 gatgctggcc ctgggcgccg gtaatggcgc ggtgttccaa ctggcgccgc agcgttttcg
      481 aaaggagatc ggcgtcatga ccggcctgat cggcatgacc ggcgggatcg gcggcttcta
      541 tctggcctcg accctgggca tggccaagaa ggcgaccggt tcctaccagc ttggctttct
      601 gggcttcgcc gccctggccg tcttcgcgct cttcgccctg catagcctga aggcccgctg
      661 gcgcgccgtc tggccgaccc tgcatgccga cgcccgcccc gtgcgcgtct gatcctggcc
      721 tcttgaaaat gagcaacgcc atgagcaaag ccccaacgcg ggaaagactg atcgttatcg
      781 gcaacggcat ggccggatgc cgggcggtcg aggaaatcct caagcgcgat cctgaccgct
      841 acgagatcgc gatcttcggg accgagcccc gcgtcaacta caaccgcatc atgctgtcgc
      901 cggtgctggc gggcgagaag acgttcgacg acatcgtcat caatgacgag gcctggtatc
      961 gcgacaacgg catcgccctg cacgtgggtc gtgcggtcat cggggtcgat ctggaaggca
     1021 agacggtgct ggccgagggc ggtctggcgc tcgcctacga caagctgatc ctggccacgg
     1081 gttcggatcc gttccgcctg cctctgccag gctccgacct gaagggcgtg gtcaccttcc
     1141 gcgatctgga cgatgtcgag gtgatggtcg ctgcctcggc caagccgggc gcccgcgctg
     1201 tgatcatcgg cggcggtctg ctgggcctgg aagccgccta cggtctggcg cgacgcggca
     1261 tgggcgcgac ggtggtccac ctgatggacg tgttgatgga gcgccagctg gacgaaagcg
     1321 ccggcttcct gctgcgcgag gctctgctgg agcgcggcgt cgagacggtg atgggtgccc
     1381 attccgagga gatcatcggc gagaacggcc aggtcaccgg cctgaaactc a
//
LOCUS       DAOMEO010000959         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9078_length_1431_cov_2.566860, whole genome shotgun sequence.
ACCESSION   DAOMEO010000959 DAOMEO010000000
VERSION     DAOMEO010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9078_length_1431_cov_2.566860"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLH31_17310"
     CDS             complement(<1..592)
                     /locus_tag="PLH31_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR90989.1"
                     /translation="MHIDLSARRVIVTGASRGIGHATAAAFAREGVRLAICSRSAETL
                     AVVADEMRALGAAEVIAVALDVRDTAAVRAFVAQVAEAWDGVDVLVNNAGQGKGGSLE
                     SLEPEDILDHANNIQVAHFRFVQAVVPHMRKQKWGRIININALAGALPTPDGIPSVTN
                     RAAGIALSKLLGQQLAKDNILVNSLNMGWIDTGQWDR"
     gene            757..1431
                     /locus_tag="PLH31_17315"
     CDS             757..1431
                     /locus_tag="PLH31_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR90990.1"
                     /translation="MTVSARRARPEAPLERSAIAEGAAASTAAVRPRRPDPRAAKTIE
                     NIVNAAQNVMMNHGTPRITAQEVCDEAGVSRGTLYRYFPSMEAVLEAVVLRLRTETDD
                     ELNHAMEGCETPSERFAAFLKYSVSNNETRRGSQFLHVEPAFVLQYFESNFDHFIARV
                     LTALESVFDAWEKDIGASVDRSAIAEMMVRLALSETVVSPREGKPLALRLQSTIDVIL
                     GALKNG"
BASE COUNT          240 a          471 c          456 g          264 t
ORIGIN      
        1 gtcgatccca ctggccggtg tcgatccagc ccatgttcag gctgttgacc agaatgttgt
       61 ccttggccag ctgctgaccc agcagctttg agagcgctat gccagccgcg cgattggtca
      121 ccgacggaat cccgtccggc gtgggcaggg cgccggccaa tgcattgatg ttgatgatcc
      181 ggccccattt ctgcttgcgc atgtgaggca cgaccgcctg cacgaagcgg aaatgggcga
      241 cctggatgtt gttggcgtgg tccaggatat cctcgggctc aaggctctcc aggctgccgc
      301 ccttgccctg accggcgttg ttgaccagca cgtccacgcc gtcccaggcc tcggcgacct
      361 gggccacgaa ggctctcaca gcggcggtgt cgcggacatc cagagcgacc gcgatgacct
      421 ccgcggcgcc cagcgcgcgc atctcgtcgg ccacgaccgc gagggtctcg gcgctgcgcg
      481 aacagatcgc cagcctaacg ccttcacggg cgaaggcagc ggccgtggcg tggccgatgc
      541 cccggctcgc gcccgtcacg atcacccgac gggcgctgag gtcgatatgc atgtcggtgt
      601 ttcctgatgg gcttctggcg agccgattgg gcctggtatt ttctagtcaa aaatcgtatt
      661 tatgtctcgt attttctcgc cgagccggcg ggccggtttg cgtgttcgat cccggcaagc
      721 tacgctgcac accgatcttt tcggagtagg atacgcatga ccgtcagcgc ccgaagggct
      781 cgtccagagg ccccactcga gcgttcggcc atcgccgagg gcgccgccgc ttcgacggcc
      841 gccgttcgcc cgcgccggcc ggatccccgc gcggccaaga cgatcgagaa catcgtcaat
      901 gccgcccaga acgtgatgat gaaccacggc acgccgcgga tcaccgctca ggaggtctgt
      961 gacgaggccg gcgtctcacg cgggacgctg tatcgctatt tcccctccat ggaagcagtg
     1021 cttgaagcgg tggtcctgcg actgcgcacc gagaccgacg acgaactgaa ccatgccatg
     1081 gagggatgtg agaccccgtc cgagcgcttc gccgcgttcc tgaaatacag cgtctcgaac
     1141 aatgagaccc ggcgaggctc gcagttcctg catgtcgaac cggcgttcgt gcttcagtat
     1201 ttcgagtcga atttcgacca cttcatcgcc cgggtgctga ccgcgctgga atcggtgttc
     1261 gacgcctggg agaaggacat cggcgcctcg gtcgatcgca gcgccatcgc cgagatgatg
     1321 gtgcgcctgg ccctcagcga aacggtcgtg tcgccgcgcg agggcaagcc tctggcgctg
     1381 cgcctccaga gcaccatcga cgtcatcctg ggcgcgctga agaacggcta g
//
LOCUS       DAOMEO010000960         1431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9079_length_1431_cov_2.558866, whole genome shotgun sequence.
ACCESSION   DAOMEO010000960 DAOMEO010000000
VERSION     DAOMEO010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9079_length_1431_cov_2.558866"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="PLH31_17320"
     CDS             complement(<1..79)
                     /locus_tag="PLH31_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR90991.1"
                     /translation="MTAAQSMAGRRRLSQAELDMIVTAHE"
     gene            complement(179..1426)
                     /locus_tag="PLH31_17325"
     CDS             complement(179..1426)
                     /locus_tag="PLH31_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR90992.1"
                     /translation="MATGHEYRRLTQYDVDVICAKHDRLWAARLGGARAVFAFCDLSG
                     LSVTGRNLCDADFTGALLVGCDMRKAKLDNANMYGADMQGADLTDASMRRVDLRGSSL
                     RGANLTGADLFEADLREGSIAAADRKEGYKIIEPVQREAYATGAVLAGANLERSRLSG
                     IMATKADFSDAILKDAKLVRANLKQANFNGANLAGADLSGANLAGADLRNTVLVGAKT
                     QSWNVNDTNMEGALTDKPSGTSVSDMPYEQMIADHARWCETGGAEGKPSSFDKADLRN
                     LKSVRGFNLTALSAKAAVFYGLDMEGVQLQGAQLEGADLRACNLRHADLRGARLRGAK
                     LSGSDLRDAQLGPLLIGRDRLLPSDLTGVILTNADLARADLRQACLSGADLSRANFTN
                     ALLKEIDITGAIRQGARGLEDVI"
BASE COUNT          230 a          475 c          478 g          248 t
ORIGIN      
        1 tctcgtgggc cgtcacgatc atgtcgagct cggcctggct gaggcgtcgt ctgccagcca
       61 tgctctgcgc ggcggtcatg ccctgatgtc tcccggaaag tacttcgctc gaacgccacc
      121 aggataccgc cgatcaacga gcgaacggtt aagactgcgc ggaaatgtcg cggtcgcgct
      181 agatcacgtc ttccaggcct ctcgccccct ggcgaatggc gcccgtgatg tcgatctcct
      241 tgaggagcgc attggtgaag ttggcgcgcg acagatcggc gccactgagg cacgcctgtc
      301 ggaggtcggc ccgcgcaaga tcggcgttgg tcaggatcac cccggtcaga tccgacggca
      361 gcagcctgtc gcgtccgatc agcaaggggc ccaactgggc gtcgcgcaga tccgaacccg
      421 acagcttggc gccgcgcagc cgcgcgccgc gcaggtcggc gtggcgaaga ttgcaggccc
      481 gcagatcggc gccctccagc tgagcgccct gaagctgcac gccttccatg tccaggccat
      541 agaacacggc agccttggcc gacagggccg tcaggttgaa gccgcgcacg gacttgagat
      601 ttcgcaggtc ggccttgtcg aacgaggacg gcttgccctc cgcgccgccg gtttcgcacc
      661 agcgggcatg gtcggcgatc atctgttcat agggcatgtc ggagacgctg gtgcccgacg
      721 gcttgtcggt cagggcgcct tccatattgg tgtcgttgac gttccaggac tgggtcttgg
      781 ccccgaccag cacggtgttg cgcaggtcgg cgcccgccag attggccccc gacagatcgg
      841 cccccgccag attggcgccg ttgaaattgg cctgtttgag gttggccctc accagcttgg
      901 cgtccttgag gatggcgtcg ctgaagtcag ccttggtggc catgatgccc gaaagacgcg
      961 agcgctccag attggcgccg gccagcacgg cgcccgtggc ataggcctcg cgctggaccg
     1021 gctcgatgat cttgtagcct tccttgcggt cggcggcggc gatcgagccc tcacgcagat
     1081 cagcctcgaa gagatcagcg ccggtcagat tggcgccccg caggctggag ccgcgcaggt
     1141 cgacgcgccg catcgaggcg tcggtcaggt cggcgccctg catgtcggcg ccgtacatgt
     1201 tggcattgtc gagcttggcc ttgcgcatgt cgcagccgac cagcagggcc ccggtgaaat
     1261 cggcgtcaca cagattgcgg cctgtgaccg acaggccaga caggtcgcaa aaggcaaaga
     1321 ccgcccgcgc cccgcccagc cgcgccgccc acaggcggtc gtgcttggcg cagataacgt
     1381 ccacgtcata ttgcgtcagg cgccgatact cgtggcctgt cgccactggg g
//
LOCUS       DAOMEO010000961         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9117_length_1427_cov_2.196793, whole genome shotgun sequence.
ACCESSION   DAOMEO010000961 DAOMEO010000000
VERSION     DAOMEO010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9117_length_1427_cov_2.196793"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1406
                     /locus_tag="PLH31_17330"
     CDS             264..1406
                     /locus_tag="PLH31_17330"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HQR90993.1"
                     /translation="MPMAVVAVTKETRGGETRVAATPETVKKLTAAGFSVVVEAGAGS
                     AASYPDADYEAAGAKLAKTAKDAITGADILFKVRAPEASEIAALKSGAIVAAALNPHQ
                     DKDTLTALAKAGASAIAMEFIPRITRAQVMDMLSSQANLAGYRAVIEGAEAYGKALPM
                     MMTAAGTIAAAKVFIMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVESLGAKFLA
                     VEDDEFKNAQTAGGYAKEMSKEYKAKQAELVSAHIAKQDIVITTALIPGRPAPKLVSA
                     EQVASMRPGSILVDLAIEQGGNVVGAKLNETVVTANGVKILGAANLPGRIATDASALY
                     ARNLFALSGLFLNKEGAFAPDFEDEILKAALVTQGGEIVHPSLKTA"
BASE COUNT          273 a          479 c          461 g          214 t
ORIGIN      
        1 gaagcgaggc gcttgacgtg ctggacgttg cgcaaacgcc ttaccgcgaa ccgggattgg
       61 cttctttggc ggagtgatcg ctggattcgt gctggcggtt ctgggcgtcg tgcttctgcg
      121 ggaaaagccc gcttctggac actgacaccg gtagacatcg gcataatttc tccctgacgc
      181 gcacgtaacg cctcgacaag aggcaagact atcccctaaa cagcagcgca agctgactgg
      241 gatagcagcg ctctgggagg ggtatgccga tggccgtcgt cgccgtgacg aaagaaaccc
      301 gcgggggtga aacgcgggtc gccgccacgc cggaaacggt gaaaaaactg accgccgccg
      361 gcttctctgt ggtggtcgag gccggcgcgg gcagcgccgc ctcctacccc gacgccgact
      421 atgaggcggc cggcgccaag cttgccaaga ctgcgaaaga cgcgatcacc ggcgccgata
      481 tcctgttcaa ggtgcgcgcc ccggaagcct ccgagatcgc cgccctcaag agcggcgcca
      541 tcgtcgccgc cgcccttaat ccgcatcagg acaaggacac gctgaccgcc ctggccaagg
      601 ccggcgccag cgctatcgcc atggagttca tcccgcgtat cacgcgggcc caggtgatgg
      661 acatgctgtc cagccaagcc aacctcgccg gctaccgcgc cgtgatcgag ggcgcagagg
      721 cctacggcaa ggccctgccg atgatgatga ccgccgccgg caccatcgcc gcagccaagg
      781 tgttcatcat gggcgtcggc gtcgccggcc tgcaggcgat cgccacggcc cgccgcctgg
      841 gcgcggtggt caccgccact gacgtgcggc cggccacgaa ggaacaggtc gagtcgctgg
      901 gcgccaagtt cctggccgtc gaggacgacg agttcaagaa cgcccagacg gccggcggct
      961 atgccaagga aatgtcgaag gaatacaagg ccaagcaggc agagctggtc tccgcgcaca
     1021 tcgccaagca ggacatcgtc atcaccacgg ccctgatccc cggccggccg gcgcccaagc
     1081 tggtcagcgc cgagcaggta gcctcgatgc gtccgggttc gatcctggtc gacctggcca
     1141 tagagcaggg cggcaatgtg gtcggcgcca agctgaacga gaccgtggtc acggccaatg
     1201 gcgtcaaaat cctgggcgcg gccaacctgc cgggccgcat cgctaccgac gctagcgcgc
     1261 tctatgcccg caacctcttt gcgctgtcgg gtctgttcct caacaaggaa ggcgctttcg
     1321 cccccgactt cgaggatgag atcctgaagg ccgccctggt cacccagggg ggcgaaatcg
     1381 tccacccgtc tctgaagacc gcctaactcg catcgaaagg gaggctc
//
LOCUS       DAOMEO010000962         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9133_length_1426_cov_1.649891, whole genome shotgun sequence.
ACCESSION   DAOMEO010000962 DAOMEO010000000
VERSION     DAOMEO010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9133_length_1426_cov_1.649891"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLH31_17335"
     CDS             <1..644
                     /locus_tag="PLH31_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQR90994.1"
                     /translation="PLPVRLVYWIGLCVAGSVVGTGVSQVIGRDGRADARPWLYGSLI
                     AIGVTLPFTLVVWLVTEVMFHGQPRLAALPYYLPPVFIITSAMTGLNFLIQRRPPETH
                     AAAAGALAPRFLDRLPPRLRGAQIRAVEAQDHYLRLHTSKGEDLILMRLSDAVAELEG
                     IEGAQTHRSWWVARDAVEAARRGDGRATLTLRGGVEAPVSRAYAKALREAGWF"
     gene            complement(641..1195)
                     /locus_tag="PLH31_17340"
     CDS             complement(641..1195)
                     /locus_tag="PLH31_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR90995.1"
                     /translation="MPVAPGPVLETERLILRPTATEDLDGWASLMADEQASRFIGGLQ
                     PRSSAWRGMASMAGSWTLLGFGMFSVIEKSSGEWLGRIGPWQPEDWPGTEVGWGLSRA
                     AWGKGYAVEAATAAIDWVFDELGWDEVIHCIDPENTPSQKVAERLGSYNRGRGQLPAP
                     FSDHVIDIWGQTRDEWRARRGAGA"
     gene            complement(1212..>1426)
                     /locus_tag="PLH31_17345"
     CDS             complement(1212..>1426)
                     /locus_tag="PLH31_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQR90996.1"
                     /translation="IYGAEAAARRYFGVGADKLTTAQAARLAAILPSPLKWKAVKPGP
                     YVKKRARKIGAATGTVRREGLAACVV"
BASE COUNT          196 a          520 c          493 g          217 t
ORIGIN      
        1 cgccgctgcc cgtgaggctg gtctactgga tcggcctgtg tgtcgccggc tcggtcgtgg
       61 gcacgggcgt gagccaggtc atcggtcgcg acggccgggc cgacgcccgg ccctggctct
      121 acggaagcct gatcgcgatc ggcgtcaccc tgcccttcac gctcgtcgtc tggctggtca
      181 ccgaggtgat gtttcacgga cagccgcgcc tggcggccct gccctattat ctgccgccgg
      241 tcttcatcat cacctcggcg atgaccggcc tgaacttcct gattcagcgc aggccgccgg
      301 agacccacgc cgccgcggcc ggggctctgg cgccgcgctt cctggatcgt ctgccgcccc
      361 gcctgcgcgg cgcgcagatc cgcgccgtcg aagcccagga ccactatctg cgcctgcaca
      421 cctccaaggg cgaggacctg atcctgatgc gcctgtcgga cgccgtggcc gaactggaag
      481 gcatagaagg cgcgcagacc catcgctcgt ggtgggtggc ccgggacgcg gtcgaggccg
      541 cccggcgcgg tgacggccgg gccaccctca ccctgcgcgg cggcgtcgaa gcgccagtca
      601 gtcgggccta cgccaaggcc ctgcgggagg cgggctggtt ctaggcgcct gcgccccggc
      661 gcgctcgcca ctcgtcccgg gtctggcccc agatgtcgat cacatggtcg gaaaacggcg
      721 cgggcagctg gccgcgtccc ctgttgtacg accccaggcg ctcagcgacc ttctgggagg
      781 gcgtgttctc cgggtcgatg cagtggatga cctcgtccca acccagttcg tcgaacaccc
      841 agtcgatggc cgccgtcgcg gcctcgacgg cgtagccctt gccccaggcg gcgcgggaca
      901 ggccccagcc gacctcggtt ccaggccagt cctccggctg ccacggcccg atccgtccca
      961 gccattcgcc gctggacttc tcgatcaccg aaaacatgcc aaagcccagc agggtccagg
     1021 agccggccat ggaggccatg ccccgccagg cggatgaacg gggttgcagg ccgccgatga
     1081 agcgcgaggc ctgttcgtcg gccatcagac tggcccatcc gtccaggtcc tcggtcgcgg
     1141 tcgggcgcag gatcaggcgc tcggtctcga gcacagggcc gggggctacg ggcatgggag
     1201 aacgctcctg actagacgac gcaggccgcc aggccctcgc gccgcacggt tccggtcgcc
     1261 gcgccgatct tgcgggcgcg cttcttgaca taggggccag gcttgaccgc cttccacttc
     1321 agcgggctgg gcaggatggc ggccagacgc gcggcctggg cggtggtcag cttgtcggcc
     1381 ccaacaccga aatagcgacg ggcggcggcc tcggcgccat agatgc
//
LOCUS       DAOMEO010000963         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9155_length_1423_cov_3.044591, whole genome shotgun sequence.
ACCESSION   DAOMEO010000963 DAOMEO010000000
VERSION     DAOMEO010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9155_length_1423_cov_3.044591"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLH31_17350"
     CDS             <1..483
                     /locus_tag="PLH31_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR90997.1"
                     /translation="VDFALAHPEAVDRLVLVGPWLSGFDASYGFLARTLKLLVLFKLG
                     AVNSAVKDPYILTKGATAPRKRVADLLRAHPGNVTAGTKEQSLAVARPRLGEIRAPTL
                     VLVGAVDIKDVQEQAKAIEAAVPGARRIVVPQSGHLMYLERPAAFAGYVIDFISEHPR
                     "
     gene            complement(621..>1423)
                     /locus_tag="PLH31_17355"
     CDS             complement(621..>1423)
                     /locus_tag="PLH31_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQR90998.1"
                     /translation="GLSASGYYAWRVRPESRRAAANRALIEDIRLIHAESCGTYGSPR
                     VHAVLRGHGRRVGRSRVERLMRRAGLRGLAALPRRTRTTDSRHGYPIAPNRLARNFQA
                     AAPGQVWLADLTYIPTGEGWLYLAGILDMHTRKLVGWSMRDTLHAQIALEALTMAIER
                     QKPAPGLIHHSDRGIQYAAETYRSALAQSGITPSMSRKGDCWDNAPMESFFHTLKTER
                     VHHRVYATRAEARRDLFGYIEGFYIYNSRRLHSALGYISPAEMERRAA"
BASE COUNT          233 a          433 c          507 g          250 t
ORIGIN      
        1 gtggatttcg ccttggccca ccccgaagcg gtcgaccgtt tggtgctggt cggcccctgg
       61 ctcagcggct tcgacgcttc ctacggcttt ctggcgcgaa ccctgaagtt gctggtcctg
      121 ttcaagttgg gcgcggtcaa cagcgccgtg aaggacccct acatcctgac caagggcgcg
      181 accgccccac gcaaacgcgt ggcggatctg ctgcgagccc atccaggcaa tgtcaccgcc
      241 gggactaagg agcagtcgct cgcggtggcc aggccgcgcc tgggcgagat tcgcgcgccg
      301 acgcttgtgc tggtgggtgc ggtcgacatc aaggatgtgc aagagcaggc caaggcgatc
      361 gaggccgcag tcccgggcgc gcgtcggatc gttgtgcctc agagcggaca ccttatgtac
      421 ctggagcgcc cagcggcttt cgccggttat gtcattgact tcatcagcga gcatccaaga
      481 tgacgaaggg gttgatttgt caaaatcgac cgtccgcttg ggtggaactt cgcgctaggc
      541 gccctgccag taagccgacc ttcaggccgc aaccgccagg aagtgtcggt cctgatcttc
      601 ccccgagaaa gtggacgggg ttaagccgct ctgcgttcca tctcggcggg gctgatatag
      661 cccagagccg agtgcaggcg acgtgaattg tagatgtaga agccctcgat atagccaaac
      721 aggtcacgcc gggcctcggc acgggtggcg tagactcgat gatggacccg ctcggtcttg
      781 agggtgtgga agaagctttc catcggcgcg ttgtcccagc agtcgccctt gcggctcatg
      841 gatggagtaa tccctgactg ggccagggcc gagcggtagg tctcggcggc gtattgaatg
      901 ccgcgatccg aatggtggat gagtccgggc gcgggctttt ggcgctcgat ggccatggtc
      961 agggcctcca gggcgatctg ggcgtgcagg gtgtcgcgca tcgaccagcc gacgagcttg
     1021 cgggtgtgca tgtcgaggat gccggccagg tagagccagc cttcgccagt cggaatatag
     1081 gtcaggtcgg cgagccagac ctgacctggg gccgccgcct ggaagttgcg ggcgagcctg
     1141 ttgggggcga tcggatagcc atgtcggctg tcggtcgtgc gggtgcgacg gggcaatgcg
     1201 gccagcccgc gcaggccggc gcggcgcatc agccgctcga cccgcgagcg tccgacacgg
     1261 cggccgtggc cgcgcagaac ggcgtggacg cgcggcgagc cataggtccc gcaactctcg
     1321 gcatggatca ggcggatgtc ctcgatcagg gcgcggttgg ccgccgcgcg ccggctctcg
     1381 ggcctgacgc gccaggcgta gtagccgctg gccgacagac cca
//
LOCUS       DAOMEO010000964         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9192_length_1418_cov_2.941306, whole genome shotgun sequence.
ACCESSION   DAOMEO010000964 DAOMEO010000000
VERSION     DAOMEO010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9192_length_1418_cov_2.941306"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /gene="rpsK"
                     /locus_tag="PLH31_17360"
     CDS             complement(<1..121)
                     /gene="rpsK"
                     /locus_tag="PLH31_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQR90999.1"
                     /translation="MAKEPGRVKKRERKNITSGVAHVNASFNNTMITITDAQGN"
     gene            complement(134..502)
                     /gene="rpsM"
                     /locus_tag="PLH31_17365"
     CDS             complement(134..502)
                     /gene="rpsM"
                     /locus_tag="PLH31_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQR91000.1"
                     /translation="MARIAGVNIPTNKRVLIALQYIHGIGQKSARDIVTKVGLDDTRR
                     VNQLTDAEVLQIRETIDKDFTVEGDLRRENSMNIKRLMDLACYRGLRHRKGLPVRGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            complement(676..1245)
                     /locus_tag="PLH31_17370"
     CDS             complement(676..1245)
                     /locus_tag="PLH31_17370"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQR91001.1"
                     /translation="MNLILFGPPAAGKGTQAKRLVTERGMVQLSTGDMLRAAIASGSD
                     LGARVKGVLDRGELVTDEIVIALIEDRLPEAEAAGGAIFDGFPRTVAQAQALDKMLAA
                     RGQKIDLVLRLKVDEPSLIERISKRFAEQGRADDNPEVFVTRLAQYNAQTAPLLPYYV
                     EQGKLTELDGMADVETVAASIDAALSGAA"
BASE COUNT          234 a          454 c          432 g          298 t
ORIGIN      
        1 tgtttccctg cgcgtcggtg atggtgatca tggtgttgtt gaacgaggcg ttcacgtgcg
       61 ccacgcccga agtgatgttc ttgcgttcgc gctttttaac gcgacccggt tccttggcca
      121 tcgtcaggct accttatttc ttcttgccgg cgatcggctt ggccggaccc ttgcgggtgc
      181 gagcgttcgt gtgcgtgcgc tgaccgcgga ccggaaggcc cttacggtga cgcaggccgc
      241 gatagcaggc caggtccatc aggcgcttga tgttcatgga gttttcgcgg cgcaggtcgc
      301 cttcgaccgt gaagtccttg tcgatcgttt cacggatctg caggacctca gcgtcggtca
      361 gctggttgac gcggcgggtg tcgtccaggc ccaccttcgt gacgatgtca cgagcggact
      421 tctggccgat gccatgaatg tactgaagcg cgatcaaaac gcgcttgttc gtcgggatgt
      481 tgacgcctgc gatacgggcc acgtctaatg ttctcctagt cacgcactaa agacgcgaac
      541 ggcgcgcccg gcccggtctt tcgacccagt ccggcgcgca cagatcgcgc cctttcgcgg
      601 aagcgccccg ttatagggat cgttgcgccg ccgtcaatgg ttgaatccgc cgaaaatcaa
      661 cgaattgaaa atgtcttacg ccgcgcccga aagcgccgcg tcgatagagg cggcgaccgt
      721 ttccacgtcc gccatcccgt ccagctccgt caacttcccc tgctccacat agtagggcag
      781 cagaggggcg gtctgggcgt tgtactgggc caaccgcgtc acgaagactt ccgggttgtc
      841 gtcggccctg ccctgctcgg caaatctctt ggagatccgt tcgatcaagg acggttcgtc
      901 taccttgagt cggaggacga ggtcgatctt ctggccgcgg gcggccagca ttttatccag
      961 cgcctgcgcc tgagctacgg tgcggggaaa tccgtcgaag atggcgccgc cagcggcctc
     1021 agcctccggc aggcggtcct cgataagcgc gatgacgatc tcgtcggtca ccagctcgcc
     1081 acggtccaga acgcccttca cgcgcgcgcc caggtcagag cctgacgcga tggccgcccg
     1141 gagcatgtcg ccggtggaaa gctggaccat gccgcgctcg gtgacgagcc tcttggcctg
     1201 agtccccttc cccgccgccg gcggtccgaa caggatcaag ttcatgaatg ccccttcgcg
     1261 cgtcgccgag tctccgctcg gtcacttccc cgcagaccta gagtgcggcg aaaaaaaacg
     1321 gaagccggtt ttgtgacaaa ccggcttccg aaacgcttta tcgttgctag cggcccttgc
     1381 cgcccctgag tttcgacttc ttgatcaggc cctcgtac
//
LOCUS       DAOMEO010000965         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9195_length_1418_cov_2.235510, whole genome shotgun sequence.
ACCESSION   DAOMEO010000965 DAOMEO010000000
VERSION     DAOMEO010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_1418_cov_2.235510"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="glyA"
                     /locus_tag="PLH31_17375"
     CDS             <1..526
                     /gene="glyA"
                     /locus_tag="PLH31_17375"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516792.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQR91002.1"
                     /translation="KKVNSAVFPGLQGGPLEHVIAAKAVAFGEALRPDFKAYARQVIA
                     NARALAEALGKSGVNIVSGGTDSHLMLVDLRPKGVTGRDAEHSLERAYMTCNKNGVPF
                     DTAPFTVTSGIRLGTPAGTTRGFMEAEFTRVGELIGEVINGLAAHGADGNAAVEAKVR
                     EEVLALTGRFPIYN"
     gene            558..1031
                     /gene="nrdR"
                     /locus_tag="PLH31_17380"
     CDS             558..1031
                     /gene="nrdR"
                     /locus_tag="PLH31_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQR91003.1"
                     /translation="MRCPFCGHAESQVKDSRPSEDGAAIRRRRACPECGGRFTTFERV
                     QLRELTILKRSGRRSPFDREKLVRSIALATQKRPVDAERVDRMINGIVRQLESMGETE
                     LPSSTVGEMVMKALKALDDVAYVRYASVYRDFKETSDFAKFLGEEGLSDVADEEV"
     gene            1102..>1418
                     /locus_tag="PLH31_17385"
     CDS             1102..>1418
                     /locus_tag="PLH31_17385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQR91004.1"
                     /translation="MVEEKIRLLIVEGRSYSGISDELLRGAVQAIEAYGADYDIITVS
                     SALQIPGAVAMAEEAGHRPVGIQYDGYVALGCVIRGETYHFEIVANETARGLQDLCIG
                     KRLA"
BASE COUNT          260 a          419 c          476 g          263 t
ORIGIN      
        1 caagaaggtc aattcggcgg tgttccccgg cctgcagggt ggtccgctgg aacacgtcat
       61 cgccgccaag gccgtggcgt tcggtgaagc cctgcgccct gatttcaagg cttatgcgcg
      121 gcaagtgatc gccaatgccc gggccttggc cgaggctctg ggcaagtcgg gcgtcaacat
      181 cgtgtctggc ggcaccgaca gccacctcat gcttgtggac ctgcgtccca agggcgtgac
      241 cggccgcgat gcggagcaca gcctcgagcg cgcctacatg acctgcaaca agaacggtgt
      301 gcccttcgac acggcgccgt tcacggtcac ctcgggcatt cgcctgggta cgccggcggg
      361 cacgacgcgg ggcttcatgg aagccgagtt tacccgggtt ggcgagctga tcggcgaggt
      421 catcaacggt ctggccgctc acggcgctga cggcaatgcc gcggtcgagg ccaaggttcg
      481 cgaggaagtc ctggccttga cgggccgctt cccgatctac aactaatagg ccgaacaaag
      541 ccgcaggggg ctagatcatg cgctgcccgt tctgtggaca cgccgaaagc caagtaaagg
      601 acagccgccc gtcggaagac ggcgcggcca tccggcgtcg tcgcgcctgc ccggagtgcg
      661 gcggacgttt caccaccttc gaacgggtcc agttgcggga actgacgatt ctcaagcgat
      721 cggggcgtcg ctcgccgttc gatcgtgaga agctggtgcg ctcgattgcc ctggccaccc
      781 agaagcgccc cgtggacgca gagcgtgtgg atcgcatgat caacggcatc gttcgacagc
      841 tggaaagcat gggcgagacc gagcttccgt cctcgacggt gggcgaaatg gtcatgaagg
      901 cgttgaaggc gctcgatgac gtggcctatg tccgctacgc ctcggtctat cgcgatttca
      961 aggaaactag cgatttcgca aagtttctgg gcgaagaagg cctgagcgac gtcgcggacg
     1021 aagaggtata ggcgcattct gtatcgattg cggctgagtc tgttggcgac gcgccagcgc
     1081 cggccctgga gttcgagtta gatggttgaa gagaagatcc gcctgctgat cgtggagggc
     1141 cgaagctatt cgggcatctc cgacgaactg ctgcgcggag ccgtgcaggc gattgaggcc
     1201 tacggcgccg actatgacat catcaccgtt tccagcgccc tgcaaatccc cggcgcggtg
     1261 gcgatggccg aagaggccgg tcatcgtccg gttggcattc agtatgacgg ttatgtcgct
     1321 ttgggctgtg tgattcgcgg cgagacctat catttcgaga tcgtcgccaa cgaaaccgcg
     1381 cgtggcctgc aggatctgtg cattggcaag cgtctggc
//
LOCUS       DAOMEO010000966         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9196_length_1418_cov_2.062362, whole genome shotgun sequence.
ACCESSION   DAOMEO010000966 DAOMEO010000000
VERSION     DAOMEO010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9196_length_1418_cov_2.062362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1335
                     /locus_tag="PLH31_17390"
     CDS             103..1335
                     /locus_tag="PLH31_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR91005.1"
                     /translation="MIRKGPPDAPQPREPDEAFEPYEEFRRIPLPVYWIAIALAIWGA
                     VTLWDNAQAVGVGREERAEQIEETPALALNSGAQVFEARCSTCHQPNAAGVRSAIPPL
                     AGSRFVAADPAVIVQILLHGIDGPIDVGGKVFDGHMPSFASVLSDAELARVASHVRQT
                     WGGDDQEITPAFVAAQRERFAGRGAWRGGEELARVVDPGLAPQPSVGPVRISSFDDPA
                     VMRLVTQGQGEAWACASCHGAAGEGGANVPRLAGLPQEYIVKQLQDYVSGRRRNETMQ
                     TVAGALSDANRRGLARYYSALRTPSTAQPELGGDMARGEQLALHGDWSKNLPACFSCH
                     GPSGFGVAPNFPSLAAQHPAYTASQLAAWVGGQRDNSDVQLMNEVARNLSDADRRAVA
                     DYLATLPPVPAVSDDERR"
BASE COUNT          213 a          510 c          475 g          220 t
ORIGIN      
        1 ggcgcgcgac cttgtcgcct atctgcgcag tctggaccgc accttccctg ccccgcccac
       61 cgccgtccgc gacgacggct acagcgaaaa gggcgagccg aaatgatccg caaagggccg
      121 cccgacgccc ctcagccccg cgaaccggac gaggccttcg aaccctacga ggagttccgg
      181 cgcattccgc tgccggtcta ttggatcgcc atcgccctgg cgatctgggg cgcggtcacc
      241 ttatgggaca acgctcaggc ggtcggcgtc ggacgtgaag aacgcgccga gcagatcgaa
      301 gagacgcccg ccctcgcttt gaacagcggc gctcaggtgt tcgaggcgcg gtgttcgacc
      361 tgccatcagc ccaatgctgc gggcgtgcgc agcgccatcc cgccgctggc gggatcccgg
      421 ttcgtcgccg ccgatccggc cgtcatcgtg cagatcctgc ttcacggcat cgacggcccg
      481 atcgatgtcg gcggcaaggt gttcgacggc catatgccga gcttcgccag cgtgctgtcc
      541 gacgccgaac tggcgcgggt cgccagccat gttcgccaga cctggggcgg cgacgaccaa
      601 gagatcacgc cggccttcgt ggcggcgcag cgcgaacgtt tcgccggccg cggggcctgg
      661 cgcgggggcg aggagctggc gcgcgtcgtc gaccccggtc tggcgcctca accatcggtc
      721 ggacctgtcc ggatctccag tttcgacgat cccgccgtga tgcgtctggt cacacagggc
      781 cagggcgaag cctgggcctg cgcctcctgt cacggcgccg cgggcgaggg cggcgcgaac
      841 gtgccccggc tcgcgggttt gccgcaggag tatatcgtca aacagcttca ggactatgtg
      901 tcagggcgcc gccgcaatga aacgatgcag actgtcgccg gcgccctgag cgacgccaac
      961 cggcgcggtc tcgcgcggta ctattcggcg cttcgcacgc cctccaccgc gcagccggag
     1021 ctgggcggag acatggcgcg tggcgagcag ctggcgctgc acggcgactg gtccaagaac
     1081 cttcccgcct gtttcagctg ccacggcccg tcgggcttcg gcgtggcgcc gaacttcccc
     1141 tccctggccg cccagcaccc cgcttatacg gcctcgcagc tggccgcctg ggtcgggggg
     1201 cagcgcgaca actctgacgt ccagctgatg aacgaggtcg cgcgcaatct gtccgacgcg
     1261 gacagacgcg cggtcgccga ctatcttgca acccttccgc cggtgccggc cgtttccgat
     1321 gatgaaaggc gatgaccatg gatgacgacg ctccgcctcc cgcaccgcag gatcggcccg
     1381 ccaagggccg gttcgcccgt gacgtgatct atgccgtc
//
LOCUS       DAOMEO010000967         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9232_length_1415_cov_1.640441, whole genome shotgun sequence.
ACCESSION   DAOMEO010000967 DAOMEO010000000
VERSION     DAOMEO010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9232_length_1415_cov_1.640441"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLH31_17395"
     CDS             complement(<1..1185)
                     /locus_tag="PLH31_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR91006.1"
                     /translation="MRHLLFAAAAVAAPAIAMAQTAPIVGDWYGTLNAGGNGLPLVFH
                     IKADGSAAVDSPAQNAMGLVATASFADGRARVTLTAIPASCEGVLSADGKTLDGKWSQ
                     GGAQLPLALGRTPSAVQAVAAPIRPQTPKPPFPYRAEEVSYLNPVSGMKLAGTLTLPQ
                     GEGPFPVALLITGSGVQDRDETIYGHKSFLVLADALTRRGVAVLRVDDRGAGGSQAGD
                     LMAATTADYATDVEAGVAFLRGRKDIDPGRVGLIGHSEGGLIAPMVAARDTKIAFIVL
                     MAGTGVPGGDVLKTQIHDITLASGAPAEKAQQQAKAIAAANAIILEEPDTAKARPRII
                     TALLDGGVPSRAAAEAQVTLLTSPWYRAFLTLDPAASLSQVRVPVLAVNGEKDTQVSA
                     KQN"
     gene            complement(1250..>1415)
                     /locus_tag="PLH31_17400"
     CDS             complement(1250..>1415)
                     /locus_tag="PLH31_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91007.1"
                     /translation="MTGAVVGVQPFGGEGLSGTGPKAGGPHALLRYAVERAVSVNITA
                     QGGDPALLNL"
BASE COUNT          202 a          478 c          514 g          221 t
ORIGIN      
        1 gttctgtttg gccgagacct gggtgtcctt ctcgccattc accgccagga ccggcacccg
       61 aacctggctg aggctggcgg cgggatccaa ggtcaggaag gcccggtacc agggcgaggt
      121 cagcagggtg acctgggcct ccgccgccgc ccggctgggg acgcctccgt ccaggagtgc
      181 ggtgatgatg cgcggcctgg ccttggccgt gtcgggctct tccaggatga tggcgttggc
      241 ggcggcgatc gccttggcct gctgctgcgc tttttccgcc ggcgctccgg aggccagggt
      301 gatgtcgtgg atctgggtct tcaggacgtc ccctccgggt acccccgtgc cggccattag
      361 cacgatgaag gcgatcttcg tatccctggc ggccaccatc ggcgcgatca gcccgccctc
      421 gctgtgacct atcaagccca cgcggcccgg atcaatgtcc ttgcggccgc gcaggaaggc
      481 gacgcccgcc tcgacgtcgg tcgcgtagtc tgcggtagtc gcggccatca ggtcgcccgc
      541 ctgcgagcca ccggcgcccc ggtcgtcgac tcgcagcacg gccacgcccc tgcgggtcag
      601 ggcgtcggcc agcaccagga aggacttgtg gccatagatg gtctcgtcac gatcctggac
      661 gcccgagccg gtgatcagca aggccaccgg gaaagggccc tcgccctgcg gcagggtcag
      721 ggttccggcc agtttcatgc ccgagacggg gttgaggtac gacacctcct cggcgcgata
      781 aggaaagggc ggctttggcg tctgggggcg gatgggcgcc gccacggcct ggaccgccga
      841 cggggtgcgg cccagggcca ggggcagctg tgcgccgccc tgactccact tgccgtcaag
      901 cgtcttgcca tcggccgaca gaacgccttc gcagctggcg gggatcgcgg tcagggtcac
      961 gcgcgccctg ccgtcggcga aagacgctgt ggcgaccaat cccatggcgt tttgggcggg
     1021 gctgtcgaca gcggccgagc cgtcggcttt gatatggaag accagcggga ggccgttgcc
     1081 gccggcgttc agggttccgt accagtcgcc gacgatcggg gcggtctggg ccatggcgat
     1141 ggcgggcgcc gcgactgcgg cggcggcgaa cagcagatga cgcataacga agaaagctcc
     1201 cccggatgac cgggagagct taagccgcgc gcgcggaggt tgtgaagacc tacaggttca
     1261 acaacgccgg gtccccgccc tgggccgtga tgttgacgct caccgcccgt tccaccgcgt
     1321 agcggagcag ggcgtggggg ccgccggcct tggggccggt gcccgacagg ccttcgccgc
     1381 cgaacggctg cacgcccacc acggcgccgg tcatc
//
LOCUS       DAOMEO010000968         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9253_length_1412_cov_2.269713, whole genome shotgun sequence.
ACCESSION   DAOMEO010000968 DAOMEO010000000
VERSION     DAOMEO010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_1412_cov_2.269713"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>430
                     /locus_tag="PLH31_17405"
     CDS             <1..>430
                     /locus_tag="PLH31_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase"
                     /protein_id="HQR91008.1"
                     /translation="EVCEIFIDMDKEVAAFRSLMNNFASAVSLGLQHGVPLDEFVDAF
                     VFTRFEPAGPVTGNDSIKSATSILDYVFRELGVSYLGRDDLANADPQEFNADGLGHGK
                     ADAPGVEPEPLPASKFISKGFSRGAATDNLVFLPFGGRKAE"
     assembly_gap    431..480
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            552..1055
                     /locus_tag="PLH31_17410"
     CDS             552..1055
                     /locus_tag="PLH31_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR91009.1"
                     /translation="MNPIARLAALGLSVAILASAAPALAQNASQIARVRGGASCPGCN
                     LFQADLSNLTLRGLNLSKARLRQADLSTVVMNRVSFAGGDLRDVNAYGGVFGGASFAR
                     ANLTNASFVGAFLEGANFAGANLTGVNFSGAEMDRAVGLSQGQLNRACGDSSTRLPRG
                     LSIPGCR"
     gene            1151..>1412
                     /locus_tag="PLH31_17415"
     CDS             1151..>1412
                     /locus_tag="PLH31_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR91010.1"
                     /translation="MSRMGVSLAVLTLALTALAGQAQASPGDKDVARMVSFGGMCVSC
                     ELSGRKLTGAKFAGANFDRAVLIGADLRGAAFYGSNFSGADLT"
BASE COUNT          205 a          462 c          432 g          263 t
ORIGIN      
        1 gaggtctgcg agattttcat cgacatggac aaggaggtcg ccgccttccg cagcctgatg
       61 aacaatttcg ccagcgcggt gtcgctgggt ctgcagcacg gcgtaccgct ggacgagttc
      121 gtcgacgcct tcgtgttcac ccggttcgaa ccggcgggac cggtgaccgg caacgactcg
      181 atcaagtcgg cgacctcgat cctcgactac gtgttccgcg aactgggcgt ctcgtatctt
      241 ggccgcgatg atctggccaa tgctgaccct caagagttca acgccgatgg actgggccac
      301 ggcaaggccg atgcccccgg cgttgagccc gaacccctgc ccgccagcaa gttcatctcc
      361 aagggcttct cgcgcggcgc ggcgaccgac aatctggtgt tcctgccgtt tggcggacgc
      421 aaggccgagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 cgccgttcgt ctggccttaa acttcggctg tcatcctgct tgcgtcgata tccgccagga
      541 agctcttcag aatgaacccg atcgcacgtc tcgccgccct cggcctgagc gtcgccattc
      601 tggcgagcgc ggcgccggcc ttggcccaga acgccagcca gatcgcccgg gttcgcggcg
      661 gcgcaagctg tccgggctgc aatctcttcc aggccgacct gtccaacctg accctgcggg
      721 gtctgaacct gtcgaaagcc cgtctgcgcc aggccgatct gtcgacggtg gtgatgaacc
      781 gggtcagctt cgccggcggt gacctgcggg acgtcaacgc ctatggcggc gtgtttggcg
      841 gggccagctt cgcccgggcc aacctgacca atgccagctt tgtcggagcc tttctggagg
      901 gcgccaactt cgccggagcc aatctgaccg gcgtgaactt ttccggcgcc gagatggacc
      961 gcgccgtggg cctgtcgcag ggccagctga accgggcttg cggcgacagc tccacccgct
     1021 tgccgcgtgg tctttccatc cctggttgcc gttaagctgc tgtcattacg gcgatttaag
     1081 tggcgctggg tcgctcgcgg ccccaaatcg taactcaagc agtcgcgttt ctgactggta
     1141 ggagatttcg atgtcccgca tgggcgtttc actcgcggtt ctgacgctgg ccctgacagc
     1201 cctggccgga caggcccagg ccagtcccgg ggacaaggac gtcgcccgca tggtctcgtt
     1261 cggcggcatg tgcgtcagct gcgagctgtc gggccgcaag ctgaccggcg ccaagttcgc
     1321 cggcgccaat ttcgaccgcg ccgtcctgat cggcgccgac ctgcggggcg cggccttcta
     1381 tggctccaac ttctccggcg ccgacctgac cc
//
LOCUS       DAOMEO010000969         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9262_length_1411_cov_3.238938, whole genome shotgun sequence.
ACCESSION   DAOMEO010000969 DAOMEO010000000
VERSION     DAOMEO010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_1411_cov_3.238938"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..695)
                     /locus_tag="PLH31_17420"
     CDS             complement(69..695)
                     /locus_tag="PLH31_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQR91011.1"
                     /translation="MYDPMATAMNLVPMVVEQTSRGERAFDIFSRLLKERIIFLTGPV
                     EDGMASLICAQLLFLESENPKKEIAMYINSPGGVVTAGLAIYDTMQYIKSPVSTVCMG
                     MAASMGSLLLCAGAPGQRISLPNSRIMVHQPSGGFRGQASDIERHAEDIIKTKRRLNE
                     IYVKHCGRTYEEVERTLDRDHFMSAEEALAWGLIDHINETRDETDAKV"
     gene            complement(869..1411)
                     /locus_tag="PLH31_17425"
                     /pseudo
     CDS             complement(869..1411)
                     /locus_tag="PLH31_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522253.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
BASE COUNT          231 a          431 c          453 g          296 t
ORIGIN      
        1 tagatcagaa ctgaaaaagc cgccgacctg gcggtcggcg gcttgatcag atttgcgagg
       61 ctcgaggcct agaccttggc gtcggtctcg tcgcgcgtct cgttgatatg gtcgatcaga
      121 ccccaggcca gggcttcctc ggcgctcatg aagtggtcgc ggtcgagcgt gcgctcaacc
      181 tcttcataag tgcggccgca atgcttgacg tagatttcgt tcaggcgacg cttggtcttg
      241 atgatgtcct cggcatggcg ctcgatgtcc gaggcctggc cgcggaaacc gcccgatggc
      301 tggtggacca tgatccgcga gttcgggagc gagatgcgct gacccggcgc gccggcgcag
      361 agcagcaggc tgcccatcga ggcggccatg cccatgcaca cggtggaaac cgggctcttg
      421 atgtattgca tggtgtcata gatcgcgagg ccagccgtca ccacgccgcc cggcgagttg
      481 atgtacatgg cgatttcctt cttggggttt tctgactcca ggaagagcag ttgagcgcag
      541 atcaggctgg ccatgccgtc ttcgacgggg ccggtcagga agatgatccg ctccttcagc
      601 aggcgcgaga aaatgtcgaa cgcacgctcg ccgcggctgg tctgttcgac caccatcggc
      661 acgaggttca tcgccgtcgc catcggatca tacatagggt tcgccctttt tcgcgttttg
      721 gggccgtgcg aatcacacct cgcgcggcgt cgctaacgca tatcgcgtgg cccttaacag
      781 gttgcaagga gccggagctc cgcttgaggc aaagaaaaag gcgcggccct cgcgagccgc
      841 gcctcattca gtgcgttggt cggaagcctt agccgccgta gccttccggc agatcgtctt
      901 cttccaggag ctcgtccttg gagacttcct tttcctcgat cttggccttg ccgaagatca
      961 ggtcgacgac cttgtcctcg tagatcgggg cgcgcagcga agcctgcagg tcggcgcgct
     1021 ggcggtacat gtcgaacacc tgctgggcct gcgcgccata ttggcgagct tcgcgcatga
     1081 tggcgtcggt cagttcctgt cggtgacgac cacgtcgttc ttgcggccga tctcggcgag
     1141 caccagaccc aggcgcacgc ggcgctcggc gatcttgcga tactcgtcct tcagctgatc
     1201 ttcggtcttg tcggcgtctt ccggcggcag gccgccttgc gacttgtcgg cctggacctg
     1261 ctgccagatg ccggcgaact cggcgtcgac catgcgcggc ggcagggcga agtcatgctt
     1321 ggtgtccagc acgtccagca gcgcgcgctt cagcttgaag cgcgacgagt tgtcgtaacg
     1381 gcccgaaagg ttggacttca gcagttcttt c
//
LOCUS       DAOMEO010000970         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9273_length_1410_cov_2.014760, whole genome shotgun sequence.
ACCESSION   DAOMEO010000970 DAOMEO010000000
VERSION     DAOMEO010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_1410_cov_2.014760"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLH31_17430"
     CDS             <1..1147
                     /locus_tag="PLH31_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HQR91012.1"
                     /translation="LPELVKRFNGGDTADAALWNVLFATTWGVMQFFCSPVLGLLSDR
                     FGRRPVILTSIFGLGVDFLFMAFAPSLWWLFIGRVFNGMTAASFSTAGAYVADVTTPE
                     NRAKGFGLMGAAFGVGFTFGPALGGFLWQFDHRLPFLVCAALALCNWLYGYFVLPESL
                     PPERRIQKFEWKKANPLGSLTLLASKPNLMGLAGVGFLFQLAHNVLPSVFVLYMGFRY
                     QWSVQTIGLTLMASGISGILLQAFLVGRIVKAVGERGALMIGLFSGFVGFLIYALAPT
                     GWLYLCGLPIFAFSGLIQPGLQGLMTRRVSPQEQGQLQGANSAMMGISSIIGPTLYLA
                     PFAWAVRNDATLHMPGLPVLIAAALMLAATGLAFRFAHPISTEPRPA"
     gene            1231..>1410
                     /locus_tag="PLH31_17435"
     CDS             1231..>1410
                     /locus_tag="PLH31_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HQR91013.1"
                     /translation="MRAHRALLPALILLAACSPEGPSNAQAIPDLAQPNRRAPSDPVS
                     MKASFAPVVKKAAPAV"
BASE COUNT          179 a          491 c          455 g          285 t
ORIGIN      
        1 gctgccggaa ctggtgaagc ggttcaacgg cggcgacacc gccgacgcgg cgctgtggaa
       61 cgtgctgttc gcgaccacgt ggggcgtgat gcagttcttc tgctcaccgg tcctgggttt
      121 gctgtcggac cgctttggcc gtcgcccggt gatcctgacc tcgatcttcg gcctgggcgt
      181 cgacttcctg ttcatggcct tcgcgcccag cctgtggtgg ctgttcattg gccgggtgtt
      241 caacggcatg acggcggcca gcttctcgac cgccggcgcc tatgtggccg acgtcaccac
      301 gccagaaaac cgcgccaagg gctttggcct gatgggggcg gcgttcggcg tcggcttcac
      361 cttcggtccg gccctgggcg gctttctgtg gcagttcgac caccgcctgc cgttcctggt
      421 ctgcgcggcc ctggcgctgt gcaactggct gtacggctat ttcgtgctgc ccgaatccct
      481 gccgcccgag cgccggatcc agaaattcga gtggaagaag gccaatccgc tcggctcgct
      541 gaccctgctg gccagcaagc ccaacctgat gggcctggcc ggcgtgggct tcctgttcca
      601 gctggcccac aacgtcctgc cgtcggtctt cgtgctctat atgggcttcc gctatcagtg
      661 gtcggtgcag accatcggcc tgaccctgat ggccagcggc atttcgggca tcctgctgca
      721 ggccttcctg gtcggccgca tcgtcaaggc tgtgggcgag cgcggcgcgc tgatgatcgg
      781 tctgttctcc ggcttcgtcg gcttcctcat ctatgccctg gccccgaccg gctggctcta
      841 tctgtgcggc ctgccgatct tcgccttctc gggcctgatc cagccggggc ttcagggcct
      901 gatgacccgc cgggtctcgc cgcaggaaca gggacagctg cagggcgcca attcggcgat
      961 gatggggata tcctcgatca tcgggccgac gctgtacctc gccccgttcg cctgggcggt
     1021 gcgaaacgac gccaccctgc acatgccggg cctgccggtg ttgatcgcgg cggctttgat
     1081 gctggcggcg acgggtctgg cgttccgctt cgcccaccct atttcaaccg aaccgcgtcc
     1141 ggcataaccg gttaggtcac ggcgcggcca tcaatgcgcc tgaaagctcg gctgatgtcc
     1201 gggccgcttc caaggagatg ctccacaccc atgcgagccc atcgcgcttt gcttcccgcc
     1261 ctgatcctgc tggcggcctg ttcgccggaa ggtccgtcca acgcccaggc gatcccggac
     1321 ctggcccagc ccaaccgccg cgcgcccagc gacccggtct cgatgaaggc ctcgttcgcg
     1381 ccggtcgtga agaaggccgc gccggccgtg
//
LOCUS       DAOMEO010000971         1409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9281_length_1409_cov_1.770310, whole genome shotgun sequence.
ACCESSION   DAOMEO010000971 DAOMEO010000000
VERSION     DAOMEO010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9281_length_1409_cov_1.770310"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>819
                     /locus_tag="PLH31_17440"
     CDS             30..>819
                     /locus_tag="PLH31_17440"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQR91014.1"
                     /translation="MELPDGFSYRVISQAGETMSDGFVVPGAFDGMGCFDLGGDRVAL
                     VRNHELDHDDIDTGPYGMGSRLAPNIARQSVYDFTEGGLPLPGGTTTVVYDLKSGQTI
                     SQHLSLAGTAVNCAGGITPWGSWLTCEETVRKAGKSVNKDHGYVFEVPSTAKGLVEPV
                     PLKAMGRFKHEAACIDPSTGVVYMTEDVGDGLFYRFLPNDRRNLAAGGRLQALGFVGV
                     ASGADSRNQTSRTIEPAAWLDARWIDLDGVDSPDDDLRRRGHAVG"
     assembly_gap    820..829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <830..1259
                     /locus_tag="PLH31_17445"
     CDS             <830..1259
                     /locus_tag="PLH31_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQR91015.1"
                     /translation="LWWGRGELYFTATSGGPKAAGQIFRYVPSADEGQVGERDNPGRL
                     QLFVESDNTRVLDYADNITISPQGYIVVCEDRYSLIKPNHIKMVTPQGKVFTLGRNVF
                     KGNAEFAGACFSPDGQTLFVNIQWPGMTLAITGPWATMKV"
     gene            complement(1296..>1409)
                     /locus_tag="PLH31_17450"
     CDS             complement(1296..>1409)
                     /locus_tag="PLH31_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HQR91016.1"
                     /translation="VWTLMAQVPLSLGVLHQAGAAVLLAAAAAFAWRVRRP"
BASE COUNT          247 a          446 c          475 g          231 t
ORIGIN      
        1 ggccgttgcg tcccgatccc aaaggcatga tggagctgcc tgacggcttc tcctaccggg
       61 tgatcagcca agcgggcgag accatgtccg acggtttcgt ggtgccggga gccttcgatg
      121 gcatgggctg cttcgacctg ggcggcgacc gtgtcgccct cgtccgcaac cacgagctgg
      181 accacgacga tatcgacacg ggtccctacg gcatgggttc acgcctggcg ccgaacatag
      241 cccggcaaag cgtctacgac tttaccgagg gcggcctgcc actgccgggc ggaacgacta
      301 cggtggtcta tgacctgaag agcggtcaga ccatcagcca gcatctcagc ctggcgggta
      361 cggcggtgaa ctgcgcgggc gggatcaccc cgtggggatc atggttgacg tgcgaggaga
      421 cggtccggaa ggcgggcaag tcagtcaaca aagaccacgg ctatgtcttc gaggtgccca
      481 gcaccgccaa ggggttggtc gagccggttc cgctgaaagc catgggccgc ttcaagcacg
      541 aggccgcctg catcgatccg agcaccggcg tggtctacat gaccgaagac gtcggcgacg
      601 gtctgtttta ccgtttcctg cccaacgacc gacgcaacct ggcggcgggc ggacggcttc
      661 aggcgctcgg cttcgtcggg gtcgcgagcg gcgctgacag ccgcaaccag accagccgca
      721 cgatcgagcc cgcggcttgg ctggatgcgc gctggatcga tctggacggc gttgacagcc
      781 ctgatgacga cctccggcgg cgcggccatg cggtcggcgn nnnnnnnnng ctgtggtggg
      841 gccggggcga gctctatttc accgcgacct cgggcggtcc aaaggccgcg gggcagatct
      901 tccgctacgt gccgagcgcc gatgagggtc aggtcggcga gcgcgataat ccaggccgcc
      961 tgcagctgtt cgtcgagagc gataatacgc gcgttctgga ctatgccgac aacatcacga
     1021 tctcgcccca gggctacatc gtggtctgcg aggatcgcta ttccctgatc aagccgaacc
     1081 acatcaagat ggtgaccccg cagggcaagg tcttcactct gggtcgcaac gttttcaagg
     1141 gcaatgccga gttcgccggc gcctgcttct cgccggatgg ccagaccctg ttcgtcaaca
     1201 tccagtggcc aggcatgacc ctagcgatca ccggtccctg ggcgacgatg aaggtttagg
     1261 gccctttccg acctgatggc ttcgtcaggg tcgggctagg gccggcgcac gcgccaggca
     1321 aaggcggcgg cggcggctag cagcaccgcc gccccagcct ggtgcaacac gccgagggat
     1381 aggggcacct gggccatcag ggtccagac
//
LOCUS       DAOMEO010000972         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9295_length_1407_cov_4.351331, whole genome shotgun sequence.
ACCESSION   DAOMEO010000972 DAOMEO010000000
VERSION     DAOMEO010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9295_length_1407_cov_4.351331"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1407)
                     /locus_tag="PLH31_17455"
     CDS             complement(119..>1407)
                     /locus_tag="PLH31_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91017.1"
                     /translation="LIQLAWDGASLDILRGDPLAAFSTYETVGRLTTNGLLYDQRRKE
                     IRLRDLGWQLAAAELHGLRYGGTGGADGDASIAGQMKPYAVGPVYNCEPTQLSAALLI
                     FQLSCSSILAVDAVRDGGVPLAFDADYPDWTALAVAAVPASTYATCLAQGLIRLGSAV
                     VYTLTVDLRGDNDTINGQAYPSTRGQIARRIATGRGTIALSDAQIDFAALNYMEQEQA
                     GTVGFYFKDAITKAQALTEVMAGCLGWWAVRANGLLALGFLEEPTRSPALTIQYPEDF
                     GSSEPQMMQTYQAPRRATYVGWQRNYTPQDPSRLAGSVDAAAALLYRGTERFASSSDG
                     FQASLWPTAAAVYVSGGFSQEAAAHAEAARQQRVMGVRRERWRVTVPCDPFANLLGKV
                     IQIYGFTRYGWAGARKFICVGMSFASSKSVALDLWG"
BASE COUNT          243 a          472 c          470 g          222 t
ORIGIN      
        1 cctcgctgcg caagcatttc ggcaacgaaa tcccgcagac catcatggaa ccagggcctc
       61 ccgttccggt gctggtcgtc gtctcctgca cgcggtcgag tatcctgtgc gccattcgct
      121 atccccatag atcgagcgcg acagacttcg acgaggcgaa gctcatgccg acacagatga
      181 acttgcgtgc gccggcccag ccgtagcgcg taaagccgta gatctggatc accttgccca
      241 gcaggttggc gaagggatcg cagggcacgg tcacgcgcca ccgctcgcga cgaacaccca
      301 tgacgcgttg ctgccgcgcc gcctcggcat gggccgccgc ctcctgcgag aagccgccgc
      361 tcacatagac cgcggccgcc gtgggccaga ggcttgcctg gaagccgtcg gacgagctgg
      421 caaagcgttc ggtgccccga tagaggagcg ctgccgccgc atcgacggag cccgccagcc
      481 ggctgggatc ctgcggcgtg taattgcgct gccagccgac ataggttgcc cgacggggcg
      541 cctggtaggt ctgcatcatc tgcggctccg aggaaccgaa gtcctcgggg tactggatgg
      601 tcaacgccgg ggaacgtgtc ggctcttcga ggaagcccag ggcaaggagg ccgtttgccc
      661 gtacggccca ccaacccagg cagcctgcca tgacctcggt cagggcctgg gccttggtga
      721 tcgcgtcctt gaagtagaaa cccaccgtgc cggcctgctc ctgctccatg tagttcaggg
      781 ccgcgaagtc gatctgggca tcggagagcg cgatcgtgcc gcgaccggtg gcgatgcgcc
      841 gggcgatctg gcctcgcgtc gagggatagg cctggccatt gatggtgtcg ttgtcgccgc
      901 ggaggtcgac cgtgagggtg taaaccaccg ccgagcccag ccgaatcaga ccctgcgcca
      961 ggcaggtggc gtatgttgag gctggcaccg cagctactgc cagcgccgtc cagtccgggt
     1021 agtcggcatc gaacgcgaga ggcacgccgc catcccgcac ggcgtccacc gccaggatcg
     1081 aactgcagga cagctggaag atcagcagcg cggcactcag ctgcgtcggc tcgcagttgt
     1141 agaccgggcc caccgcatag ggcttcatct gcccggcgat cgaggcgtcg ccatcggcgc
     1201 cgccagtacc gccatagcgc aggccatgca gctcggccgc ggccagttgc cagccgaggt
     1261 cgcgcagcct gatctccttg cggcgctggt cgtagagcag gccattggtg gtgagccgtc
     1321 cgacggtctc ataggtcgag aacgcagcca gcggatcgcc ccgcaggata tcaaggctgg
     1381 cgccgtccca cgcgagttgg atcaacc
//
LOCUS       DAOMEO010000973         1402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9354_length_1402_cov_2.449889, whole genome shotgun sequence.
ACCESSION   DAOMEO010000973 DAOMEO010000000
VERSION     DAOMEO010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9354_length_1402_cov_2.449889"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLH31_17460"
     CDS             complement(<1..487)
                     /locus_tag="PLH31_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HQR91018.1"
                     /translation="MRLAPTLFALAFLAIALPVAAQPNAGFQVIQVPDPQGAPIEVGV
                     WYPTGATPTAPARGMGNIPVAQGAPLEGQKLPLVVMSHGNGGWFGGHYDTAIALAKAG
                     FVVAALTHTGDNYKDQSRALDMANRPRQLHVLIDYMLADWTQHASLDPGRIGAFGFSS
                     GG"
     gene            609..1385
                     /locus_tag="PLH31_17465"
     CDS             609..1385
                     /locus_tag="PLH31_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQR91019.1"
                     /translation="MDRTAPPLLGTARDWAIDLAVATVIGLFFGLVGPFGSFLNGSAA
                     VRIAHWVLSTWCAVLIFGLVARLGLAAAERWRIPAVVTLGAGLLIGAAPLAAMVALSA
                     RSFWPDLSGLSALDWYGQCLIISTPSLAAYVAVRWALTRDQRQTAPPVLSILEPSPSS
                     AATLDEVLCLRMEDHYVRIHSAVGSRLVAGPFERVIASLGQREGMRVHRSWWVARSAV
                     VGVKADGRNLKLELSNGLSAPVSRASVAKLRQVGWLPSDS"
BASE COUNT          193 a          444 c          506 g          259 t
ORIGIN      
        1 agccgcctga cgagaagccg aacgcgccga tccgcccggg atcaaggctc gcatgctgcg
       61 tccagtcggc cagcatgtag tcgatcagca catgcagctg acgaggccgg ttggccatgt
      121 cgagggcgcg gctctggtcc ttgtagttgt cgccggtatg ggtgagggcg gcgaccacga
      181 agcctgcctt ggccagggcg atggcggtgt cgtagtggcc gccaaaccag ccgccatttc
      241 catgcgacat caccaccagc ggcagcttct ggccctcaag cggcgcgccc tgggcgaccg
      301 ggatattgcc catgccccgg gccggcgcgg tcggtgtggc gccggtgggg taccagacgc
      361 ccacctcgat cggcgcccct tgcggatcgg gaacctggat cacctgaaac ccggcattgg
      421 gctgagcggc gacgggtaaa gcgatggcga gaaaggcgag ggcgaaaagg gtcggggcga
      481 gccgcatggg cgatctcctg ttgcgatgac aggagcaggc ttggcccggc aaaacggcgc
      541 tctccagcgc gctttcgcga atgggcgaac gggttcgcga agcggcgaac tgtgcttata
      601 ggacggtgat ggacaggact gcgccaccgc tgctgggaac cgctcgcgat tgggcgatcg
      661 atctggccgt ggccacggtg atcggcctgt tcttcggcct ggtcggtccg tttggcagtt
      721 tcctgaacgg ctcggctgcg gtgcggatcg cccactgggt gctgtcgacc tggtgcgcgg
      781 tgctgatctt cggcctggtg gcgcgcctgg gcctggccgc cgcggagcgc tggcggatac
      841 ccgctgtggt gaccctgggc gccggcctgc tgatcggcgc cgcgccgctg gcggcgatgg
      901 tggcgcttag cgcgagaagc ttctggccgg acctgtccgg cctgagcgcg ctggactggt
      961 atgggcagtg cctgatcatc tcgacgccct cgctggcggc ctatgtcgct gtgcgttggg
     1021 ccctgacccg cgatcagcgc cagaccgccc cgcccgtgct ctcaatcctt gagccgtccc
     1081 cctcatccgc cgcgaccctg gacgaagtgc tgtgcctgcg gatggaagac cattatgtgc
     1141 gcatccattc cgccgtcggc tctcgactgg ttgctggacc gtttgagcgg gtgatcgcta
     1201 gcctcggcca gcgagagggg atgcgcgttc atcgctcctg gtgggtggcc cgctcggcgg
     1261 tggttggggt caaggccgat ggtcgcaacc tcaagcttga gctttcgaat ggcctctcgg
     1321 cgccagtctc gcgcgcgtcc gtggccaagc ttcggcaagt tggatggctg ccgtcggatt
     1381 cctagttttc ggcccgcggc gc
//
LOCUS       DAOMEO010000974         1400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9376_length_1400_cov_1.946468, whole genome shotgun sequence.
ACCESSION   DAOMEO010000974 DAOMEO010000000
VERSION     DAOMEO010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9376_length_1400_cov_1.946468"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLH31_17470"
     CDS             complement(<1..456)
                     /locus_tag="PLH31_17470"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91020.1"
                     /translation="MRTLLQQSIERLLADAVTPDVLRAAEAGVWPADLWGKIEELGLP
                     LAAVPEDLGGAGASWTDIFVVVRACGAAAAPAPLPEAILANSLLAAAGLEPPESGAIV
                     FAAGTADAPLPEVPWGRHAKWLALHDTRTGRIALHALAEARIVLDNNVAG"
     gene            complement(456..>1400)
                     /locus_tag="PLH31_17475"
     CDS             complement(456..>1400)
                     /locus_tag="PLH31_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91021.1"
                     /translation="GHDASPFERFVVNEELLAAGAPVTAHWIADRQSAPLLLRHGTEA
                     QKQRYLPAICAGEMYFCIGMSEPGSGSDLASVRTKADRQTDGSWLLNGRKVWTTNADR
                     CQAMIALVRTDPASERHAGLSQILVDLTLPGVMIRPIKDLSGHEHFNEVTFDDVRLGA
                     DALIGVEGDGWAQATSELAFERSGPERFLSAMTLFRCLVGAVGPAPDARQAVEIGRLV
                     AWLISLRSMSISVTAQLASGQDPVWAASCVKDLGTGYEQETVEVAMRLIDSLPPGAQA
                     TTTRRVLAIVQSMAPSFSLRGGTREILRGILARGLGVR"
BASE COUNT          227 a          469 c          492 g          212 t
ORIGIN      
        1 gccggcgaca ttgttatcga ggacgatccg cgcttcggcc agggcgtgga gcgcgatcct
       61 gcctgtacgg gtgtcatgaa gcgccagcca tttggcgtgt cgaccccaag ggacctccgg
      121 caagggcgcg tcggcggtgc cggcggcgaa gacgatggcg ccgctttcag gcggctcgag
      181 gccggcggcg gccagcaggc tgttggccag aatggcttcg ggcaggggcg cgggcgccgc
      241 cgcagctcca caggcgcgaa cgacgacgaa gatgtcggtc caactggcgc ccgcgccgcc
      301 tagatcttcg ggaacggcgg ccagcggcag gcccagttcc tcgatcttgc cccagagatc
      361 cgccggccac acgccggctt ccgccgcgcg aagtacatct ggcgtgacgg cgtcggccag
      421 cagccgctcg atgctttgct gaagcagggt tcgcatcagc gcactcccag tccgcgcgcg
      481 aggatgccgc gcaggatttc tcgcgtgccg ccacgcaggg agaaggacgg agccatgctc
      541 tggacgatcg ccagcacccg gcgcgtggtc gtggcttgcg cgcccggcgg aagggaatcg
      601 atcaaccgca tcgccacctc gacggtctcc tgttcatagc cagtgccaag gtccttgacg
      661 caggaagcgg cccagaccgg gtcctggcct gacgcgagct gcgccgtgac cgagatcgac
      721 atcgaccgca atgaaatcag ccaggcgacc aggcgtccga tctcgacggc ctgtcgcgcg
      781 tctggcgccg gtcccacggc gccgaccagg cagcggaaca gggtcatggc gctgaggaac
      841 cgctccgggc cgctgcgttc gaacgccagt tccgacgtcg cctgggccca cccgtcgccc
      901 tcgactccga tcagggcgtc ggcgcccaaa cggacgtcgt cgaaggtgac ctcgttgaag
      961 tgctcgtggc cggacaggtc cttgatcggc cggatcatca cgcccggaag agtcaggtcg
     1021 acgaggatct gggacaggcc cgcatggcgc tcgctggcgg gatcggtgcg gaccagggcg
     1081 atcatcgcct ggcaccggtc ggcgttggtg gtccagacct tccggccatt cagcaaccac
     1141 gagccatcgg tctggcgatc ggctttcgtt cgcaccgagg ccaggtcgga gccggatccg
     1201 ggctcgctca tgccaatgca gaaatacatt tccccggcgc agatggccgg caggtaacgc
     1261 tgcttttgcg cctccgtacc gtggcgcaag agcagggggg cgctttggcg gtcggcgatc
     1321 cagtgcgccg tgacgggggc gccggcggcc agaagttcct cattgaccac gaaccgttcg
     1381 aacggcgagg cgtcgtggcc
//
LOCUS       DAOMEO010000975         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9380_length_1399_cov_2.971726, whole genome shotgun sequence.
ACCESSION   DAOMEO010000975 DAOMEO010000000
VERSION     DAOMEO010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9380_length_1399_cov_2.971726"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLH31_17480"
     CDS             <1..513
                     /locus_tag="PLH31_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQR91022.1"
                     /translation="VKPAGETSLQHMLAEMTQAIYADFRYGKRFEKGVQTPLTTLENR
                     GGTCRDFAVLMIEAVRRLGLAAQFVSGYIHSPSRKQVAVERTGGGHTHAWLRVYLPSC
                     GWVEFDPTNGIVGNTDLIRVAVVRDPRQAPPVHGTWAGFPTDYLGMDVEVDVTSIEDH
                     LAPSLATAFG"
     gene            581..>1399
                     /locus_tag="PLH31_17485"
     CDS             581..>1399
                     /locus_tag="PLH31_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQR91023.1"
                     /translation="MRRLLRETTMLIRYGYEITVYCQQPTAMVTLLGLRDGREADVRI
                     PESFFTTPEVATSTYRDMFGNPCRRLVAPAGLLTLWSDSVMEDSGAVDPAAPDAEQHD
                     VADLPDDCLVYLMGSRYCETDRLSQLAWDRFGPIQGGWARAQAICDFVHSHISFDYKT
                     ACSTRTAYEVHEGRIGVCRDYAHLAVALCRCLNIPARYVNGYLGDIGVPRVDPMDFSA
                     WMEVYLGGKWWAFDPRNNIPRIGRIVVAHGRDAADVAMLTSFGPHQLNGFRVWAY"
BASE COUNT          230 a          470 c          442 g          257 t
ORIGIN      
        1 gtcaagcctg cgggcgagac cagcctgcag cacatgctgg ccgagatgac ccaggcgatc
       61 tatgccgact tcagatacgg caagcgcttc gagaaggggg tccagacgcc cctgaccacc
      121 ctggaaaacc ggggcggcac ctgtcgcgac ttcgcggtgc tgatgatcga ggccgtgcgg
      181 cgcctgggcc tcgccgccca gttcgtgtcg ggctacatcc acagcccctc gcgcaaacag
      241 gtggcggtcg agcggactgg aggcggccac acccatgcct ggttgcgggt ctatctgccc
      301 tcatgcggct gggtggagtt tgatcccacc aatggcatcg tcggcaacac cgacctgatc
      361 cgcgtcgccg tggtccgcga tccgcgccag gcgccacccg tccatggcac ctgggcaggc
      421 tttccgaccg actatctggg catggatgtc gaggtcgacg tcaccagcat cgaagatcat
      481 ctggcgccgt ccctggccac ggccttcggc tgacgcatct ttcgatcgcg tcgcgcgttg
      541 gcgttgaact tctacttaaa tcccggattc gccacccctg gtgcgccggc tcctgcgtga
      601 gacgacgatg ctgatccgct atggctacga gatcacggtc tactgtcagc aacccacggc
      661 gatggtgacg ctgctgggcc tgagggacgg acgcgaggcc gacgtacgca tccccgagtc
      721 gttcttcacg acacccgagg tcgcgacctc gacctatcgc gacatgttcg gcaatccttg
      781 ccgccgcctg gtcgccccgg caggcctgct gaccctctgg agcgacagcg tcatggaaga
      841 tagcggcgcg gtcgatcccg cggcgccaga cgccgagcaa cacgacgtcg ccgacctgcc
      901 cgacgactgt ctcgtctatc tgatgggcag ccgctattgc gagacggacc gtctcagcca
      961 gctggcttgg gacaggttcg gccctatcca ggggggctgg gcgcgggccc aggcgatctg
     1021 cgacttcgtc cacagccaca tcagttttga ctacaagacc gcctgctcga cacgcacggc
     1081 ctatgaggtg catgagggcc gcatcggcgt atgtcgcgac tatgcccatc tggctgttgc
     1141 gctctgtcgt tgcctgaaca tcccggcgcg ctatgtgaac ggttatctgg gcgacatcgg
     1201 cgtgccgcgc gtcgacccga tggacttcag cgcctggatg gaagtttatc tgggcggcaa
     1261 atggtgggcc ttcgacccgc gcaacaacat cccacgtatc ggccgcatcg tggtggccca
     1321 tggccgtgac gccgccgacg tggccatgct gacctcgttc gggccgcatc agctgaacgg
     1381 cttccgggtc tgggcctat
//
LOCUS       DAOMEO010000976         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9382_length_1399_cov_2.613839, whole genome shotgun sequence.
ACCESSION   DAOMEO010000976 DAOMEO010000000
VERSION     DAOMEO010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_1399_cov_2.613839"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLH31_17490"
     CDS             <1..1130
                     /locus_tag="PLH31_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91024.1"
                     /translation="SLFLANMSHELRTPLNGVIAIGDRLAEEEDPKVRRDLASLVASS
                     GRLLEQVLGDILDVSKIEAGQFQLTPAPFDLERLVRGMAELHAAAAEAKGLDFAWSIA
                     PDAAGGYLGDAGRISQILSNLLANAVKFTSKGSISLTVERRDEQLVFTVADTGVGFDD
                     TVRERLFKRFVQADQSITRQFGGTGLGLSISAALAEMMGGRIDARAVLGSGATFSVEL
                     PLPPCEDQQADARRAPADSMSLAGLRVLVAEDHPTNRKVVEVVLEPFGVVLTMVEDGK
                     AALTAVETQDFDAILMDMQMPVMDGLTATRAIRAREAANGLSPVLIIMLTANAMEEHV
                     ASAKAAGADLHLAKPLHPVMLLEALAGVRAGAAADTVSAAE"
BASE COUNT          206 a          474 c          487 g          232 t
ORIGIN      
        1 agtcgctgtt cctggccaat atgagccacg agctgcgtac gccgctgaac ggcgtcatcg
       61 ccatcggcga tcgcctggcc gaggaagagg atcccaaggt gcgtcgcgac ctggcgtcgc
      121 tggtcgcctc gtctggccgc ctgctggagc aggtgctggg cgacatcctg gatgtctcga
      181 agatcgaggc cggccagttc cagctgaccc ccgcgccgtt tgatctggag cgtctggtgc
      241 gcggcatggc cgagctgcac gccgccgccg ccgaggccaa gggtctggac tttgcctggt
      301 ccatcgcgcc cgacgcggcg ggcggctatc tgggcgacgc cggccggatc agccagatcc
      361 tgtccaacct gctggccaac gccgtcaagt tcaccagcaa ggggtcgatc tcgctgacgg
      421 tcgaacgccg cgacgagcaa ctggtcttca ccgtggccga caccggggtg ggctttgacg
      481 acacggtgcg cgagcggctg ttcaagcgct tcgtccaggc cgaccagtcc atcacccgtc
      541 agtttggcgg caccggcctt ggcctgtcga tctcggccgc cctggccgag atgatgggcg
      601 gccgcatcga cgcccgggcg gtgctgggct ccggggccac cttctcggtc gagcttccct
      661 tgcccccgtg cgaggatcag caggccgacg cacgccgggc gccggcggac tcgatgtccc
      721 tggcgggcct gcgcgttctg gtcgccgaag accatcccac caaccgcaag gtcgtcgaag
      781 tggtgctgga accgttcggc gtggtcctga ccatggtcga ggacggcaag gcggccctga
      841 ccgcggtcga gacccaggac ttcgacgcca tcctgatgga catgcagatg ccggtcatgg
      901 acggcctgac cgccacccgg gcgatccggg cgcgcgaggc ggctaacggc ctgtcgccgg
      961 tgctgatcat catgctgacg gccaacgcca tggaagagca cgtcgcctcg gccaaggcgg
     1021 ccggcgccga cctgcacctg gccaagccgc tgcacccggt catgctgctg gaggccctgg
     1081 cgggggtgcg ggccggggcc gcggctgata cggttagcgc cgcggagtag gggctgttcc
     1141 ggctcagtga ttggcaagca agaacggctt tcgagccgtc tcgtcatccc ggggacaagc
     1201 cccgggatga cgggtctcga gagggaggaa gcgtttgtct gctcccgacc ctagtgagac
     1261 gttccgtttg cccggaaaga cgccgacggc ctacgccccc tccgtcacga tgcgtattcg
     1321 catcgcgcca cctcccccgt ttcacggggc aggagggtga tgctgttctt ctcctgcccc
     1381 gtagcgaagc gtgcggggg
//
LOCUS       DAOMEO010000977         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9384_length_1399_cov_2.489583, whole genome shotgun sequence.
ACCESSION   DAOMEO010000977 DAOMEO010000000
VERSION     DAOMEO010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_1399_cov_2.489583"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..488)
                     /gene="rpsT"
                     /locus_tag="PLH31_17495"
     CDS             complement(213..488)
                     /gene="rpsT"
                     /locus_tag="PLH31_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQR91025.1"
                     /translation="MANNPGAKKAIRKIARRTEVNTARRSRVRTFLRKFEDALAGGDV
                     AVAKSAFIEAQSELMRAVSKGVVHPNTGSRKVSRLAARLKKLDKAAA"
     gene            complement(637..>1011)
                     /locus_tag="PLH31_17500"
     CDS             complement(637..>1011)
                     /locus_tag="PLH31_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR91026.1"
                     /translation="LGVAPGIGGTQRLTRFVGKSKAMDMILTGRMMGAEEAERAGLVS
                     RIFPADMLLAEVLAMAAKIASQSPLAVAMNKELVNAAYETTLTTGVALERRLFHSLFA
                     FEDQKEGMAAFVEKRKPDFKGR"
     assembly_gap    1012..1021
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1022..>1399)
                     /locus_tag="PLH31_17505"
     CDS             complement(<1022..>1399)
                     /locus_tag="PLH31_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR91027.1"
                     /translation="AGVVLIRLNRPEALNALNAALLGELAQALGQAQADDGVGCIVLT
                     GSAKAFAAGADIKEMSDKTYAEMFKADFFTAGARAIEQCRKPIIAAVSGYALGGGCEL
                     AMMCDVIIAADTAKFGQPEINLG"
BASE COUNT          248 a          448 c          443 g          250 t
ORIGIN      
        1 aacggaaaac ttgcgtccgg gatcggatat ttctgttgac tcgcgagagc ccctcgcctg
       61 gcaagggaat agttaagtcc gctctttttt ggacgttgtt atttctgaaa agcgtgcagt
      121 cgaacacaaa gcaaaagccg gttggactgc tccaaccggc ttctgtaaat acttgaaatt
      181 cttggccgaa cgtcgttcgt ccagaatttg gactaggcgg ctgccttgtc cagcttcttc
      241 agacgggcgg ccaggcggga cacctttcgc gaacccgtgt tcggatgaac cacgcccttg
      301 gaaacggcgc gcatcagctc cgattgagcc tcgataaagg cgctcttggc gacggcgacg
      361 tcgccgcccg ccagggcgtc ttcaaacttg cggaggaacg tgcgcacgcg cgaacggcga
      421 gcggtgttca cctcggtgcg acgggcgatc ttgcggatcg ctttcttggc gccgggatta
      481 ttggccatag gtaggacctt caaacggaca gccgcgcggg acagccgcga agcagggaaa
      541 atcttgagcg cgcggatata cgggaaccct tggccggggt caacgggctg tggcgaccaa
      601 tctcatctgg gtgcggccag aggtcgcatc ccagcctcag cgccccttga agtcgggttt
      661 gcgcttctcg acgaaggccg ccatcccctc cttctgatcc tcgaaggcga acagcgagtg
      721 gaacaggcgt cgctccaggg ccacgcccgt ggtcagggtc gtctcatagg cggcgttgac
      781 cagctccttg ttcatggcca cggccagggg cgactggctg gcgatcttgg ccgccatggc
      841 cagcacctcg gccagcagca tgtcggccgg gaagatccgc gacaccagac cggcgcgctc
      901 ggcctcctcg gcgcccatca tccgcccggt caggatcatg tccatggcct tggacttgcc
      961 gacaaagcgc gtcaggcgct gggtgccgcc gatgccgggg gcgacgccca gnnnnnnnnn
     1021 ncgcccagat tgatctcggg ctggccaaac ttggcggtgt cggcggcgat gatgacgtcg
     1081 cacatcatgg ccagctcgca acccccgccc agggcgtagc ccgacacggc ggcaatgatc
     1141 ggcttgcggc actgctcgat ggcgcgcgcc ccggcggtaa agaagtcggc cttgaacatc
     1201 tccgcatagg tcttgtccga catctccttg atgtcggccc ccgccgcaaa ggccttggcc
     1261 gagccggtca ggacgataca gcccacgccg tcgtcggcct gggcctggcc caacgcctgg
     1321 gccagctcgc ccaacagcgc cgcgttcagc gcattgagcg cctcgggccg gttgagccgg
     1381 atcagcacga cgccagcgg
//
LOCUS       DAOMEO010000978         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9385_length_1399_cov_2.313244, whole genome shotgun sequence.
ACCESSION   DAOMEO010000978 DAOMEO010000000
VERSION     DAOMEO010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9385_length_1399_cov_2.313244"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLH31_17510"
     CDS             <1..413
                     /locus_tag="PLH31_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR91028.1"
                     /translation="AFLSDDPEVALMHGPTFMANPLACAAANASLDLFEDGAWRGEVA
                     RVSAGLAQGLEPCRSAPGVVDVRVFGAIGVVEFEAPVAVGDLCARFAEMGVWIRPMGK
                     LIYLTPAFTTSDADLALLTRAIRKVVGVGSPLAD"
     gene            410..1346
                     /locus_tag="PLH31_17515"
                     /pseudo
     CDS             410..1346
                     /locus_tag="PLH31_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT          174 a          446 c          505 g          274 t
ORIGIN      
        1 aggccttcct gtctgacgat cccgaggtgg ccctgatgca cggcccgacc ttcatggcca
       61 atccgctggc ctgcgcggcg gccaacgcct cgctcgatct cttcgaggat ggcgcctggc
      121 ggggcgaggt agcgcgggtc tcggccggtc tggcgcaagg tctggagccg tgccgctcgg
      181 cgccgggggt cgtcgatgtg cgggttttcg gcgcgattgg tgtcgtcgag ttcgaggcgc
      241 cggtggccgt cggcgacctg tgcgcccggt tcgccgaaat gggcgtgtgg atccggccga
      301 tgggcaagct gatctatctg accccagcgt tcacgacgtc cgacgccgat ctggcgttgc
      361 tgacgcgcgc catccgcaag gttgtcggcg tcggcagccc cctggcagat tgaccggcgc
      421 ggcgcggtct ggtccaagcg gccatgcccg ccaactcgcc cctgtctggt ctggaacgcc
      481 tcgccatcct ggcgatcatc ctgacctggg gcgtgaacaa tgccgccgcc aaggtggcga
      541 ccgagcatct gccgccgatg atggtgggcg gcctgcggtt tggcctggcc ctgctgttcc
      601 tgttcccgtt catcaagccg ccattccccg agccccggaa gctgctggcg atcattctgc
      661 tgtcgggacc gatccatttc gccctggtct atattgcttt cggcatgggc cagtcgctga
      721 gcccgctggt ggtggccagt cagctatgga ttccgttcac ggcggtggcg gcgtggaaaa
      781 tgctgggcga aaccatgcga ccagcggcgg tggccggcct ggtcatcgcc tttgtgggcg
      841 tggcctggat gagcctggat ccgcacggcg ccggtgatct gccggccgtg gcgttggtga
      901 tcctggcctg cggttgctgg gcggtggcca cggtgctggt gcgcatgacc ccgggcgtca
      961 agcccttgaa ggtgcaggga ctgaccgcgc tggtcgcgac cccgaccctg atcgccatgt
     1021 ctttcgcctt cgagccgcgc gtggtcgagc gggtgagcag cgcgccgcta atcgcctggg
     1081 gcgccgtcgc ctttgccggc atcgtctcga caatcggggc cagcgccctg ctgttctggc
     1141 tggtccagcg acgcgaagcc gggcgcgtga cgccgtattt cctgatgacg cctctggtgt
     1201 cctgcacgat tggcatcctg ttcatggggg atcgggtgac cccgcaactt ctgatcggcg
     1261 gggcggcgac catggtgggt gttgctctgg tggccttgac cgagaagcgg gccgccaaga
     1321 cggcggtgct ggcggagacg gtctagcgag acacggcttc gcgacctcgc gcgggaaccg
     1381 ccgagttgat ggaaagggt
//
LOCUS       DAOMEO010000979         1399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9388_length_1399_cov_1.967262, whole genome shotgun sequence.
ACCESSION   DAOMEO010000979 DAOMEO010000000
VERSION     DAOMEO010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9388_length_1399_cov_1.967262"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PLH31_17520"
     CDS             complement(<1..1079)
                     /locus_tag="PLH31_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR91029.1"
                     /translation="MRLILLLAGLGLANAAQADPLTFNAAQELALQSPALAATAADLD
                     AAKSQGKAAGRLPDPKLRVGLDNFPISGPPAGTFGGDSMTMVSVGLMQDRPSAAKREA
                     ARQAARAEVTTAGARQALTARETQVAAALAWIDLFYTDQKLAALAAVERAITPLRDTA
                     PTAVTTGDARPGQALEAQQKLAALADRRSELLAGRARAAAELTRWTGEPGPQPIGPAP
                     DFLINAEALRAGLSDHPALRALDAARQTAGAQLAQARAEAYSDTSWDLAYQKRDGAYG
                     DMVSVGVTFGLPLFGQARREPLIAAQSARIRGAESQKAAGERQLLAALETDLADHQMH
                     HEQFARAQETLAPLAQQRADLETAS"
BASE COUNT          177 a          453 c          553 g          216 t
ORIGIN      
        1 tagctggcgg tctccaggtc ggcgcgttgc tgagccagcg gcgccaaggt ttcctgggcg
       61 cgggcaaact gttcgtggtg catctggtgg tcggcgaggt cggtctccag cgcggcgagc
      121 aattggcgct cacctgcggc cttttgactt tcggcccccc ggatcctggc gctttgggct
      181 gcgatcagcg gctcgcgccg ggcttggcca aacagcggca agccgaaggt caccccgacc
      241 gacaccatgt cgccataggc gccgtcgcgc ttctggtagg cgagatccca gctggtgtcg
      301 gaataggctt cggcgcgcgc ttgggccaac tgggcgccgg ccgtctggcg cgccgcgtcc
      361 agggcgcgga gagcaggatg gtctgacagg cccgcgcgca gggcctcggc gttgatgaga
      421 aagtccggag ccggcccgat gggctgaggt ccaggctcgc ctgtccaacg ggtcagttcg
      481 gcggcggcgc gggcccggcc ggccagcagt tcgctgcgtc gatcggccag tgcggccagc
      541 ttctgttgcg cttccagggc ttgacccgga cgggcgtctc cggtggtgac ggcggtgggc
      601 gcggtgtcgc gcagtggcgt gatcgcccgt tcgaccgcag ccagggcggc cagcttctga
      661 tcggtgtaga acaggtcgat ccaggccagg gccgcggcca cttgcgtctc gcgcgccgtc
      721 agggcctggc gggcgccggc ggtcgtgacc tccgcccgag ccgcctgacg cgcggcctcg
      781 cgcttggcgg cgctgggccg gtcctgcatc aggccgacgc tgaccatggt catgctgtcg
      841 ccgccaaagg tcccggccgg tggaccggag attgggaaat tgtccaggcc gacgcgcagc
      901 ttggggtcgg gcaacctgcc cgccgccttg ccctgggact tggccgcatc gagatcggcc
      961 gccgtcgcgg ccagggccgg cgattgcagg gccagctctt gggcggcgtt gaaggtcagt
     1021 gggtcggcct gggcggcgtt ggccagccca aggcccgcga gaagcaggat aagacgcatg
     1081 aaggagtcct gtcggatcgt gcgaacgacg tcggcgaaaa ggccagacgg gatgcctcgg
     1141 cggggcgttt gcagccgccc cgccgaggcc ggccgtctag gcggcgggag gggatcgccc
     1201 gccgcctggg ttcagccggt ccctagcctt ggggcgaggc ggcggcgggc tgggccgcgc
     1261 cggccttgcg gggcatcatt ccgcagtcgg ccggcatgtc gcagacgcga accgcggtgc
     1321 gaaagacatc cttggggttg gacggcgggg cctccgcgcg cagatggctg caggcgggga
     1381 aggtggcggc gacggcggc
//
LOCUS       DAOMEO010000980         1398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9390_length_1398_cov_2.430380, whole genome shotgun sequence.
ACCESSION   DAOMEO010000980 DAOMEO010000000
VERSION     DAOMEO010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9390_length_1398_cov_2.430380"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="hmgA"
                     /locus_tag="PLH31_17525"
     CDS             <1..1104
                     /gene="hmgA"
                     /locus_tag="PLH31_17525"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQR91030.1"
                     /translation="RLRPSAGHGRYEPYAQPTLASGPFDGPVTPNRLRWDPIAVPDAP
                     TDFVDGLSTLAGNGDVATQAGIGIHLYLANTSMQNRVFQNADGELLIVPQQGVLTLVT
                     ELGVLTAGPGEIALIPRGVRFRVEVAGSSRGYVCENYGAAFKLPDLGPIGANGLANPR
                     DFETPVAAFEDVEASTQVIQKFQGGLWASQWDHSPLDVVAWHGNFAPCKYDLARFNTI
                     NTVSFDHPDPSIFTVLTSPSEIPGTANCDFVIFPPRWMVAEHTFRPPWFHRNVMSEFM
                     GLVHGAYDAKAGGFSPGGASLHNAMSDHGPDLASWQGAVAADLKPHRIENTMAFMFES
                     RWVIRPTRFALEHSSLQADYDACWSGFPKAVLP"
     gene            1130..>1398
                     /locus_tag="PLH31_17530"
     CDS             1130..>1398
                     /locus_tag="PLH31_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HQR91031.1"
                     /translation="MKLASLKGGRDGRLVVVSNDLAWFTDAATIAPTLQAALDNWEHC
                     EPLLRGLAESLEHGGVPKERFHEHDAASPLPRAYQWLDGSAYVNHV"
BASE COUNT          228 a          477 c          454 g          239 t
ORIGIN      
        1 cgcctgcggc ccagcgccgg tcacggtcgc tacgagccct acgcccagcc gaccctggcc
       61 agcggtccgt tcgacggccc ggtgacgccc aaccgcctgc gctgggaccc cattgcggtt
      121 ccagacgcgc cgaccgattt cgtcgatggc ctgagcaccc tggctggcaa tggcgacgtg
      181 gcgacccagg ccgggatcgg catccatctc tatctcgcca acacctcgat gcagaaccgg
      241 gtgttccaga acgccgatgg cgagctcttg atcgtgccgc agcagggcgt gctgaccctg
      301 gtcaccgagc tgggcgtcct gaccgccggc cccggcgaga tcgccctgat cccgcgcggc
      361 gtgcggttcc gggtcgaggt cgcggggtct tcacggggct atgtctgcga gaactacggc
      421 gcggcgttca agctgccgga tctgggtccg atcggcgcca acggcctggc caatccgcgt
      481 gatttcgaga cgccggtggc ggccttcgag gatgtcgagg cctcgaccca agtgatccag
      541 aagttccagg gcggcctgtg ggccagccaa tgggatcata gcccgctgga cgtggtggcc
      601 tggcacggca atttcgcgcc ctgcaaatac gatctcgccc gcttcaacac gatcaacacg
      661 gtcagcttcg accatcccga tccgtcaatc ttcacggtgc tgacctcgcc cagcgagatc
      721 ccgggcaccg ccaattgcga cttcgtgatc ttcccgccgc gctggatggt ggccgagcac
      781 acgttccggc cgccgtggtt ccaccgcaac gtgatgagcg agttcatggg tctggtgcac
      841 ggcgcctatg acgccaaggc tgggggtttc tcgccgggag gggcatcctt gcacaacgcc
      901 atgagcgatc acggccccga tctggccagc tggcagggcg cggtggcggc ggatctgaag
      961 ccgcacagga tcgagaacac gatggccttc atgttcgaga gccgctgggt gatccgccca
     1021 acgcgatttg ctctggagca ttcgtcgctt caggccgact atgacgcctg ctggtcgggc
     1081 ttccccaagg ccgtcctgcc ataaccataa cgataaagag gaagcgccca tgaagctcgc
     1141 gtctctcaaa ggcggccgtg acggccggct ggtggtggtc tccaacgacc tggcctggtt
     1201 taccgacgcg gcgaccatcg cgcccaccct gcaggcggcg ctggataact gggagcattg
     1261 cgagcctctg ttgcgcggcc tggccgagag ccttgagcac ggcggcgtgc ccaaggagcg
     1321 cttccacgag cacgacgccg ccagcccctt gccgcgcgcc tatcagtggc tggacggcag
     1381 cgcctatgtg aaccacgt
//
LOCUS       DAOMEO010000981         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9401_length_1397_cov_2.147541, whole genome shotgun sequence.
ACCESSION   DAOMEO010000981 DAOMEO010000000
VERSION     DAOMEO010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9401_length_1397_cov_2.147541"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLH31_17535"
     CDS             <1..798
                     /locus_tag="PLH31_17535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR91032.1"
                     /translation="DPFGVFGGVGEPQAAFVIAPEGSTADYLEAVRAASPKKIKNYRR
                     LDHKLEREMGQVRLVAPDTSRASFDLLIAWKREQLQRTGLHDFLRPDWTSDLLTDLFE
                     RQTGDFQGLMIGLYAGDELVAGHFGVRLGGVYHPWIASTNPAYGAWSPGQLFFLRAIA
                     AMPELGLTRYDLGPGHDHYKRAYAFTQTPVQSGAVMAANVAGLMACSVESAWNLAGAG
                     GNGVVARLRRRMDVINSVELTMGGQVRGLVDAVAGQSRRRVGAHEAG"
     gene            798..>1397
                     /locus_tag="PLH31_17540"
     CDS             798..>1397
                     /locus_tag="PLH31_17540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR91033.1"
                     /translation="MIHVTVMIPTQRRLGGLAVAVRSVLAQRSVDFAALEIIVVDNDQ
                     VPSAQSTVEDLAQGAACLVHYVHEPRAGVANARNAGMARASGALIAFLDDDEEAPEGW
                     LAALLAAQARFDADVVFGPVRGRAPDSVVEHRDYLERFFSRLGPAEAGVIDHYYGCGD
                     SLLRRAALPDPVSPFAIERNQIGGEDDMLFGQMQAAGARF"
BASE COUNT          192 a          479 c          490 g          236 t
ORIGIN      
        1 gaccctttcg gggtgttcgg cggcgtgggc gagccgcagg ccgccttcgt gatcgcgccc
       61 gagggctcga ccgccgacta tctggaggcc gttcgcgccg ccagccccaa gaagatcaag
      121 aattaccgcc ggctggacca caagctggaa cgggaaatgg gccaggtgcg cctggtcgcg
      181 cccgacacct cgcgcgcgtc gtttgacttg ctgatcgcct ggaagcgcga gcagctgcag
      241 cgcaccggcc tgcacgactt tctgcgcccg gactggacca gcgacctgct gaccgacctg
      301 ttcgagcgcc agaccggcga cttccagggc ctgatgatcg ggctttatgc cggcgacgag
      361 ctggttgcgg gccatttcgg cgtgcggctg ggcggggtct atcatccgtg gatcgcctcg
      421 accaacccgg cctatggcgc ctggtcgccg gggcagctgt tcttcctgcg cgccatcgcg
      481 gccatgcccg agctgggcct gacccgctac gaccttggcc cgggccatga ccactacaag
      541 cgcgcctacg ccttcaccca gacccctgtc cagagcggcg ccgtgatggc cgccaacgtg
      601 gctggcctga tggcctgctc ggtcgagagc gcctggaacc tggcgggcgc gggcggcaat
      661 ggcgtggtgg cgcgtctgcg tcgccggatg gacgtgatca atagcgtcga actgaccatg
      721 ggcggccagg tgcgcggtct ggtcgacgcc gtggcgggac agtcacgccg ccgggtcggc
      781 gcgcacgagg ccggctgatg atccatgtga cggtgatgat cccgacccag cggcggctcg
      841 gcggtctggc cgtcgcggtc cgctccgtgc tggcccagag gagcgtggat ttcgccgccc
      901 tggagatcat cgttgtcgac aatgaccagg tcccgtcggc ccagtccacg gtcgaggacc
      961 tggcccaggg cgcggcctgt cttgttcact acgtgcacga accccgcgcc ggggtagcca
     1021 acgctcgcaa tgctggcatg gccagggcgt ccggcgcgct gatcgccttt ctcgacgatg
     1081 acgaagaagc gccggagggc tggctggcgg ctctgctggc ggcccaggcc cgctttgacg
     1141 ccgacgtggt ttttggcccg gtccggggca gggcgcccga cagcgtcgtt gagcatcgcg
     1201 actatctgga acggttcttc tcgcgcctgg gccccgccga ggccggcgtc atcgatcact
     1261 attacggctg tggcgacagc ctgctgcgcc gcgccgcctt gcccgatccc gtctcgccct
     1321 tcgcgatcga gcgcaaccag atcggcggcg aggacgacat gctgtttggc cagatgcagg
     1381 ccgccggcgc gcgtttc
//
LOCUS       DAOMEO010000982         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9403_length_1397_cov_2.026080, whole genome shotgun sequence.
ACCESSION   DAOMEO010000982 DAOMEO010000000
VERSION     DAOMEO010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_1397_cov_2.026080"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /gene="fusA"
                     /locus_tag="PLH31_17545"
     CDS             complement(<1..1363)
                     /gene="fusA"
                     /locus_tag="PLH31_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQR91034.1"
                     /translation="MARSHPLKLYRNIGIMAHIDAGKTTTTERILYYTGKSYKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWNDHRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDGVAGVEPQSETVWRQADKYNVPRMCFINKLDRTGANFKYCVQTIIDRLGAKPA
                     VLYLPIGAEADFKGLVDLVEQRAIIWKDEALGAEFFYEEIPADMADEAAEYRNNLIEL
                     AVEQDDEAMEAYLEGNEPDVATLKALIRKGTLGQAFVPVLCGSAFKNKGVQPLLDAVV
                     DYLPSPIDIEDVQGINPDTDEPDSRPTADDAPFSALAFKIMNDPFVGSLTFTRIYSGT
                     LTKGTYLNSVKDKKEKVGRMLLMHSNNREDIDAAYAGDIVAIAGLKETTTGDTLCSEK
                     QPIILERMEFPEPVIELSVEPKTKADQEKMGIALNRLSAEDPSFRVSTDHESGQTIIK
                     GMGE"
BASE COUNT          246 a          413 c          428 g          310 t
ORIGIN      
        1 gttcgcccat gcccttgatg atcgtctggc ccgattcgtg atcggtcgaa acgcggaacg
       61 aagggtcttc ggccgagaga cggttgagcg cgatgcccat cttctcctgg tcagccttgg
      121 tcttcggctc caccgacagt tcgatcaccg gttcggggaa ttccatgcgc tcaaggatga
      181 tcggctgctt ttccgaacac agggtatcac ccgtggtcgt ttccttcaga ccggcgatgg
      241 cgacgatgtc accagcgtag gctgcatcga tgtcctcacg gttgttggag tgcatcagca
      301 gcatacggcc gaccttctcc ttcttgtcct tcaccgagtt caggtacgtg cccttggtca
      361 gcgtgcccga atagatgcgg gtgaaggtca gcgagcccac gaacggatcg ttcatgatct
      421 tgaacgccag tgccgagaac ggcgcatcgt cagcagtcgg acggctgtcc ggctcgtcgg
      481 tgtcggggtt gatgccctgc acgtcttcga tgtcgatcgg cgaaggcaga tagtctacca
      541 ccgcgtcgag caggggctga acgcccttgt tcttgaacgc cgaaccgcac agcaccggca
      601 cgaaggcctg gccgagcgta cccttgcgga tcaacgcctt cagcgtcgcc acatcaggct
      661 cgttgccctc aagatacgct tccatcgcct cgtcgtcctg ctcgacggca agctcgatga
      721 ggttgttgcg atattccgcg gcttcatcag ccatgtcggc cggaatctct tcatagaaga
      781 attcagcgcc cagcgcctca tccttccaga tgatcgcgcg ctgttcgacg aggtcaacca
      841 agcccttgaa gtcagcttca gcgccgatcg gcagatacag caccgcaggc tttgcaccca
      901 agcggtcgat gatcgtctgg acgcagtact tgaagtttgc gccggtgcgg tcgagcttgt
      961 tgatgaagca catgcggggg acgttgtatt tgtcggcctg gcgccagacg gtttcggact
     1021 ggggttcgac cccggccacg ccgtcaaagg cagcgacagc gccgtccaac acgcgcagcg
     1081 agcgttcgac ttcgatggtg aagtcgacgt gcccgggggt gtcgatgatg ttcaggcggt
     1141 ggtcgttcca gaagcaggtc gtggcggccg acgtgatcgt gatgccgcgt tcctgctcct
     1201 gttccatcca gtccatcgtg gcggcgccgt catggacttc gccgatcttg taggacttgc
     1261 cggtataata aagaatacgc tcggtcgtag tcgtcttgcc ggcgtcgata tgggccataa
     1321 tgccgatatt gcgatacagt ttgagcggat ggctgcgggc catgatgctt ctccgttgcc
     1381 ggcgcaccgg cggttgg
//
LOCUS       DAOMEO010000983         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9413_length_1396_cov_2.929157, whole genome shotgun sequence.
ACCESSION   DAOMEO010000983 DAOMEO010000000
VERSION     DAOMEO010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9413_length_1396_cov_2.929157"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLH31_17550"
     CDS             <1..428
                     /locus_tag="PLH31_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intermediate filament-like cell shape
                     determinant CreS"
                     /protein_id="HQR91035.1"
                     /translation="AVERRAGDLNVALERALDRIRSLEDEGEGLRQRHAGVDTARAAA
                     IERADQLAKAAVAHDKTLQRAEERANQLRVRLEAMQEAQDAARRQHEEKVGELQAQIE
                     RLTSEAALAEGALEAARRDRSRLQMALLGATEGDIAEAS"
     gene            complement(501..1028)
                     /locus_tag="PLH31_17555"
     CDS             complement(501..1028)
                     /locus_tag="PLH31_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQR91036.1"
                     /translation="MKLWISVAALALTAAAARAEVMDATPSGFQVKHAVEIAAPAVRV
                     WASLLQPATWWDSSHTWSGSAANLSLAAASGGCFCERLPGGGSVLHMSTVYAQPGQKL
                     TLFGALGPLQSSGAAGHMSFGLAETDGKTTLTVTYDVGGYMKGGLDSIAAPVDGVIGQ
                     QVVRLKRQIETGKPG"
     gene            1284..>1396
                     /locus_tag="PLH31_17560"
     CDS             1284..>1396
                     /locus_tag="PLH31_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91037.1"
                     /translation="MTTSTPDWAARFSERMTRVRASEIRELLKLLDQPDILS"
BASE COUNT          241 a          499 c          458 g          198 t
ORIGIN      
        1 aggccgtcga acgccgggct ggcgatctga atgtcgcgct ggaacgggcc ctggaccgga
       61 tccgctcgct ggaagacgag ggcgagggcc tgcgtcaacg ccatgctggc gtcgacaccg
      121 cccgcgccgc cgccatcgaa cgcgccgacc agctggccaa ggccgccgtg gcccacgaca
      181 agacgctgca gcgggccgag gaacgcgcca accagctgcg cgtccgcctg gaggccatgc
      241 aggaagccca ggacgccgcg cgccgtcagc acgaggaaaa ggtcggcgaa ctgcaagcgc
      301 agatcgaacg cctcacctcc gaagcggcgc tcgccgaggg cgccctggaa gcggcccgcc
      361 gcgaccgctc gcgtctgcag atggctctgc tgggcgccac cgaaggcgac atcgctgaag
      421 ccagctagat ctttcccccg accaaagtcc ggaaaagggc ggtcctcgtg gccgcccttt
      481 tttgttgcgg ccgccgcgcc ctagcctggc ttacccgtct cgatctggcg cttgagccga
      541 acgacctgct gtccgatcac gccatcgacc ggcgcggcga tgctgtcgag accgcccttc
      601 atgtagccgc cgacgtcgta ggtgacggtc agggtggtct tgccgtccgt ctccgccaga
      661 ccaaagctca tgtgaccggc cgcgccgctg ctctgcagcg gacccagcgc gccaaacagg
      721 gtcagcttct ggccgggctg ggcgtagacg gtgctcatgt gcagcaccga gccgccgccc
      781 ggcagccttt cgcagaaaca gccgcccgac gccgccgcca gcgacagatt ggcggccgag
      841 cccgaccagg tatgggacga gtcccaccag gtcgccggct ggaggagcga ggcccagacc
      901 ctgacggccg gcgcggcgat ctcgacagcg tgcttcacct gaaagccgct gggcgtggcg
      961 tccatgacct cggcgcgggc ggccgcggcg gtcagagcca gggcggcgac ggaaatccag
     1021 agcttcatgc gatgtcccct tgtgacgacg gcagggttcc cccgccacca gaatgacgtc
     1081 aagcttcagt ggttttcttc cgggaatgat cgcgctaggc tcggcgcatg aacacgaccc
     1141 agggcggtcg tcggggcttc gggctccgtc gctcgcagga ccttatccgc tccgtatgac
     1201 gccctgaact gatcaggcga gacgccgcgc atgcggctcc aggacggccc cgccgtcagc
     1261 gaaacatacg gaaattcaga gacatgacga cctccacccc cgattgggcc gcgcgtttca
     1321 gcgaacgcat gacccgcgtc cgcgccagcg agatccgcga gcttctcaag cttctggacc
     1381 agcccgacat cctgtc
//
LOCUS       DAOMEO010000984         1396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9415_length_1396_cov_2.662938, whole genome shotgun sequence.
ACCESSION   DAOMEO010000984 DAOMEO010000000
VERSION     DAOMEO010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_1396_cov_2.662938"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..672
                     /locus_tag="PLH31_17565"
                     /pseudo
     CDS             265..672
                     /locus_tag="PLH31_17565"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
     gene            1069..>1396
                     /locus_tag="PLH31_17570"
     CDS             1069..>1396
                     /locus_tag="PLH31_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR91038.1"
                     /translation="MKTTAEGARLASVTVADAKSEAQRSGEVVGRAIRAMGQIEDSSR
                     QIGQIIGVIDEIAFQTNLLALNAGVEAARAGEAGRGFAVVASEVRALAQRSAEAAREI
                     KALISES"
BASE COUNT          284 a          386 c          443 g          283 t
ORIGIN      
        1 gtggataatc ggcattggct tgttaaacaa tgtcaaatcg caaatgatat gccgttttaa
       61 gaaggcggcc tccgaggtca ataataatat aatctataca caaggatata tatccttgtg
      121 tatagatact tcaatataca atgcgtagca aattattgag gtgaatagtt tgttttatat
      181 attgctgctc gatgaactca ggtttcaaat atttggggta ttctgatgga tcagcaagac
      241 tctctatatg atcgccttaa gttcatgaag attgatgctt ccgcgcaatc gagcctccag
      301 ggcgttaaat cgatcctgat gcgggagctg cctgcggcgc tggatggctt ttacgcgcaa
      361 gttcaaggcc atccggagac ccgccgtttc ttcaccgatc ccgcgcgcgt gggcagcgcc
      421 aagagcaggc agattggtca ttgggacgcg atttccagcg gccgtttcga cgaaacctat
      481 gttcgcgctg tcacggctgt tggtgaaacc catgcgcgaa tcggcctgga gccccgctgg
      541 tacatcggcg gttatgccct tctgctgaat gatctgatcg gagccgtggt ggcggagcgg
      601 tggccaaggg gaggcttcgg cgccaagaag gggcctgggg ccgatacggt cgccgccgaa
      661 ctcggggccc tggccaaggc gacgctcctt gacgtggatt tctccatctc cgtctatctg
      721 gaggctgcgg aggccgctcg caaacacgct gaagccgagg tcatggggca agagcgcgcg
      781 tcggtcgtac gatcagtcgg cgcgggcatg gccgctctgg cggcaggtga tctcacctac
      841 cgtatgcctg acgacctcgc gcccgagtat cgccaactcc ggcaagactt caaccttgcc
      901 ctgggccgga tcgaggagac gatttccact gtcgccacca cgaccggtgg cattggcgtt
      961 agcgctgagg agatggccaa ggcttcggac gacctttccc ggcggaccga gcagcaagcg
     1021 gccggccttg aagagacagc ggcggccctg gacgagatca cggcgacggt gaagaccact
     1081 gcggagggcg cgcgcctggc cagcgttacc gtggcggacg ccaagagcga ggcccagcgc
     1141 tccggcgagg tcgtcggacg ggcgatcaga gccatggggc agatcgaaga ctcgtcgcga
     1201 cagatcggcc agatcatcgg cgttatcgat gagatcgcct ttcagaccaa ccttctggcc
     1261 ctcaacgccg gcgtcgaggc cgcccgagct ggcgaagcag gccgggggtt tgctgtcgtc
     1321 gcctcggagg ttagagccct ggcccaacgt tcggccgagg cggcgagaga gatcaaggcg
     1381 ttgatctcgg agtcct
//
LOCUS       DAOMEO010000985         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9425_length_1395_cov_2.656716, whole genome shotgun sequence.
ACCESSION   DAOMEO010000985 DAOMEO010000000
VERSION     DAOMEO010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_1395_cov_2.656716"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..>1395
                     /locus_tag="PLH31_17575"
     CDS             280..>1395
                     /locus_tag="PLH31_17575"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQR91039.1"
                     /translation="MSWPPPCLWPRRRLSRRSAASSLSLATQTKVRILFGSDLVAGRS
                     APSLSGRFTAAEALARLLAGSDIEVRQTQPGVLILRPRRVPAAYVPPSGDMILAGAGA
                     VGQTAIQSDPAAPAPNGASDETVILSEVVVGSHIRGGRNGASPVLVVGREDMDRAGYA
                     TVADALTALPQVFTGSASDDAGAANTDPTTTNGTRATGVNLRGLGADATLVLINGRRM
                     AGAGLMADFADVSAIPMAAVERVEILLDGASAVYGSDAVGGVVNIVMRERYDGAETRM
                     RVGGSTHGDLGQRQIGQTFGRAWASGSLLLSGEYQRRDRLRATARDYTGEADLRPYGG
                     TDHRFNFSQPGNIMGIDPISKLLVPVYAIPAGQSGVGL"
BASE COUNT          196 a          504 c          469 g          226 t
ORIGIN      
        1 tctttcagcc gggcgacacc aacgagttcg tcagcgcggt gacggggctt ttccacctga
       61 cggccgtgcg ccgttcggac ggcgcgatcg agctgcggcg gacgccttcg gcctgacacc
      121 aaaaatattt cacaacctta ctaccaagag atgcgttctc ccgtgtctca cccgtaccgc
      181 gtctgcggca ggagcgcgtc agcaagacgt cgcccgcagg cgcccggggg gtttgtatgg
      241 tgcgcttgtc attggatttg ctggtcggcg cttcggcgct tgtcctggcc tccaccgtgt
      301 ctctggccgc gcaggcggct gagccgccgg tccgcagcgt cgtctctgag cctggcgacc
      361 cagaccaagg ttcgtatcct gttcggcagc gacctggtcg cgggccgctc cgcgccctcg
      421 ctcagcggcc ggttcaccgc cgccgaagcc ctcgcccggc tgctggccgg cagcgatatc
      481 gaggtccggc agacacagcc cggcgtgctc atcctgcggc cgcgccgcgt tccggccgcc
      541 tatgttccac cgtccggcga catgatcctg gccggcgcgg gcgccgtcgg ccagacggcg
      601 atccaatcgg atcctgccgc cccggcgccg aacggcgcga gcgacgagac ggtgatcctg
      661 tccgaagtcg tggtcggcag ccacattcgc ggcggtcgca acggcgcttc gccggtgctg
      721 gtggtcgggc gcgaggacat ggaccgcgcg ggctacgcca ccgtcgccga cgccctaacc
      781 gccctgcccc aggtgttcac cgggtcggcc agcgacgacg ccggcgcggc caataccgat
      841 cccaccacca ccaacggcac gcgcgccacg ggcgtcaatc tgcgcggtct gggcgctgat
      901 gcgaccctgg tgctgatcaa cggccgccgc atggccggcg ccggcctgat ggccgatttc
      961 gcggacgttt cggcgatccc gatggccgcc gtcgagcggg tcgagatcct tctcgacggg
     1021 gcgtcggccg tctatggctc ggacgcggtc ggcggtgtgg tcaacatcgt catgcgcgag
     1081 cgctatgacg gcgccgagac ccgcatgcgg gtcggcggct cgacccacgg agacctcggc
     1141 caacgtcaaa tcggccagac cttcggccgc gcctgggcca gcggctcgct gctgctgagc
     1201 ggcgaatatc agcgccgcga tcgtctgcgg gccacggcgc gtgactatac gggcgaagcc
     1261 gacctgcgcc cctatggcgg caccgaccac cggttcaact tcagccagcc cggtaacatc
     1321 atgggcatcg atccgatcag caagctgctg gtcccggtct acgcgatccc ggccggtcaa
     1381 tcgggcgtgg gccta
//
LOCUS       DAOMEO010000986         1395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9426_length_1395_cov_2.591791, whole genome shotgun sequence.
ACCESSION   DAOMEO010000986 DAOMEO010000000
VERSION     DAOMEO010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9426_length_1395_cov_2.591791"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLH31_17580"
     CDS             <1..223
                     /locus_tag="PLH31_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HQR91040.1"
                     /translation="LTAAGRTVLYDDTDARGGAKFATMDLIGLPWQMIIGPKGIAEGV
                     VEIKNRATGERSTASLESVLAGLTQSKAG"
     gene            223..>1395
                     /locus_tag="PLH31_17585"
     CDS             223..>1395
                     /locus_tag="PLH31_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQR91041.1"
                     /translation="MSDLRAAGPFSRWERSVARRYLFAKRKNGGVALISVISFCAVML
                     AVFALITVMSVMNGFRAELLGRILGFNGHLYAQSPLINGPDRDGVISRIRAVPGVIQA
                     VPMVEAQAMVIGPSQVTGAIVRGVTPGDLRGLKIIADNIKKGSMSGFGQGEFGGDIVL
                     IGDRMALNLGVAPGDAITLISPSGPATAFGTSTREKDYIVGGVFSVGMSQFDEAYVYM
                     PLAQSQLFFGRDTAVDYVEIKVEDPDDAKLLKPAVEAASGPGALVADWMDKNHSYFTA
                     LQVERKVMRLILFCIVAIATLNIISSLVMLVKNKGKDIAILRTIGAGQGSILRIFLMA
                     GASIGVAGTLAGLLLGVVFCAYITPIQNFVEWVTGTAVFSADVYMLSHIPAKIDWAE"
BASE COUNT          233 a          455 c          453 g          254 t
ORIGIN      
        1 gctgaccgca gccggccgca ccgtacttta cgacgacacc gacgcccgcg gcggcgccaa
       61 gttcgccacc atggatctga tcggtctgcc ctggcagatg atcatcggtc ccaagggcat
      121 cgccgagggc gtggtcgaga tcaagaaccg ggcgactggc gaacgctcca ccgcgtcgct
      181 ggagtccgtg ctggcgggtc tcacgcaaag caaggctggc taatgagcga tctcagggcg
      241 gccggaccgt tcagccgctg ggagcggtcc gtcgcgcggc ggtacctgtt cgccaagcgc
      301 aagaatggcg gcgtcgccct gatctcggtg atctcgttct gcgcggtgat gctggcggtc
      361 ttcgccctga tcaccgtcat gagcgtgatg aacggcttcc gggccgagct gctcgggcga
      421 atcctcggct ttaacggcca cctctacgcg caatcgccgt tgatcaacgg tcccgaccgc
      481 gacggcgtga tcagccgcat ccgagctgtt ccgggggtga tccaggccgt gccgatggtc
      541 gaggcccagg ccatggtcat cggtccgagc caagtgactg gcgcgatcgt tcgcggcgtc
      601 acgcccggtg atctgcgcgg gctgaagatc atcgctgaca acatcaagaa gggttcaatg
      661 tctggcttcg gccagggcga gttcggcggc gacattgtcc tgatcggcga ccgcatggcc
      721 ctcaatctcg gcgtcgcgcc gggcgacgcc atcaccctga tctcgccctc tggccccgcg
      781 acggcgttcg ggacctcgac gcgcgagaag gactacatcg tcggcggcgt cttcagtgtc
      841 ggcatgagcc agtttgacga ggcgtacgtc tatatgccgc tcgcgcagtc gcagctgttc
      901 ttcgggcgcg acaccgccgt cgactatgtc gagatcaagg tcgaagaccc tgacgacgcc
      961 aagctgctga aacccgcagt cgaggctgcc agcggcccgg gcgcgctggt cgccgactgg
     1021 atggacaaga accacagcta cttcacggcg ctgcaggtcg agcgcaaggt gatgcgcctg
     1081 atcctgttct gcatcgtcgc gatcgccacc ctgaacatca tttccagtct ggtcatgctg
     1141 gttaagaaca agggcaagga catcgccatt ctccggacca taggggccgg gcagggctcg
     1201 atcctgcgca tcttcctgat ggcgggcgcc tcgatcggcg tggccggcac cctggcgggt
     1261 ctcctgctcg gcgtggtgtt ctgcgcctac atcacgccca tccagaactt cgtcgagtgg
     1321 gtgacgggca cagccgtgtt cagcgccgac gtctacatgc tctcgcacat ccctgcgaag
     1381 atcgactggg ccgag
//
LOCUS       DAOMEO010000987         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9452_length_1393_cov_1.869208, whole genome shotgun sequence.
ACCESSION   DAOMEO010000987 DAOMEO010000000
VERSION     DAOMEO010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_1393_cov_1.869208"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLH31_17590"
     CDS             complement(<1..651)
                     /locus_tag="PLH31_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQR91042.1"
                     /translation="MAAPRDGLITQISVEEGAHVERGAVLAQLDQDRTALLAIQAVAE
                     TAQLQAASRAAQARALAAQTDAARLTRLASADAATRREAEQARQAAAVAVAQGEEAVR
                     AVDVARVRERLASLEQTIRTVRAPVTGLVLRRAATVGAAAVTTAAAPLFVLAPDGPRI
                     VRAELDEAFAARVGPGATAWITDASGGGHVFRARVVRVSPAFEAAALEDQPGARADG"
     gene            complement(831..>1393)
                     /locus_tag="PLH31_17595"
     CDS             complement(831..>1393)
                     /locus_tag="PLH31_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR91043.1"
                     /translation="RLDPSVRNGEGAAYFTARLVDPTQARSVVADLREVGRQRGFEIM
                     TRQTFADRSVRYWVAESGAGVAFVFGSAVALLVAVIVTSQTLAAAVAASLKDYAALRA
                     LGFTLGALRSIVLAQTAWIAAAGVVLAGVMTALLVGLARLKATPLILTPSMAAGVIGL
                     VVLVALGSGLWALRRVARADPAALLL"
BASE COUNT          258 a          502 c          468 g          165 t
ORIGIN      
        1 gccgtcggcg cgcgcgccgg gctgatcctc tagggcggca gcctcaaaag ccggagaaac
       61 cctcacgacg cgcgcgcgaa acacatgacc gccgccgctg gcgtcggtga tccaggcggt
      121 cgctcccggc ccgacccgag cggcgaaagc ttcgtccagt tcggcgcgaa cgatgcgcgg
      181 tccatcgggc gccaggacaa acagaggcgc tgccgccgtg gtcactgcgg ctgcgccgac
      241 ggtcgccgca cgtcgcagca ccaagccggt taccggcgcc cggaccgtcc ggatggtctg
      301 ttcgagactc gccaaccgct cacgaactcg cgccacatca acggctcgga cagcctcctc
      361 gccttgcgcg acggcaacgg ccgccgcttg ccgcgcctgt tcggcctcgc gccgtgtcgc
      421 ggcgtcggcc gaggccaagc gggtcaggcg agccgcatcg gtttgggcgg ccagggctcg
      481 ggcttgggcg gcgcgcgatg ccgcctgtag ctgggcggtt tccgccaccg cctggatggc
      541 cagtaacgca gtgcgatcct ggtcgagctg ggccaagacc gcgccgcgct cgacgtgcgc
      601 cccctcctcc acgctgatct gggtaatgag accgtcgcgg ggcgcggcca cggagatcag
      661 accgccatcg accgcgacga caccccgcgc gacagcgaca gcagggggaa gggtctgggc
      721 aaccgccgtg tccggccccc gttgacagcc cgtgagcgct agacccgcca gagtcgtcag
      781 aagaaccaag gggcgacagc gtcgcgaaac agcgcaagcc atggagaacc tcatagaagc
      841 agcgcggcgg gatcggccct tgcgaccctg cgcaatgccc aaagcccgga acccaaggcg
      901 accaacacca ccaaaccgat cacgcccgca gccatcgacg gggtcaggat caggggcgtc
      961 gccttcagac gagcaagacc caccaggagg gctgtcatga caccggccaa tacgacgccg
     1021 gccgcggcga tccaggcggt ctgggccaga acgatggagc gcagggcgcc gagggtgaag
     1081 cctagcgcgc gcagagcggc atagtcctta agcgaagcgg cgaccgccgc cgctagagtc
     1141 tgactggtga cgatcaccgc caccagcagc gccacggcgc tgccgaacac gaaggccacc
     1201 ccggctcccg actcagcgac ccaatagcgg acgctgcgat cagcaaaggt ctgccgggtc
     1261 atgatttcga agccacgttg gcggccgacc tccctaagat cggcgaccac cgagcgcgcc
     1321 tgggtcgggt cgaccaatcg ggccgtgaaa taggcagcgc cctccccgtt ccgcaccgat
     1381 ggatcgagcc gga
//
LOCUS       DAOMEO010000988         1392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9467_length_1392_cov_2.005984, whole genome shotgun sequence.
ACCESSION   DAOMEO010000988 DAOMEO010000000
VERSION     DAOMEO010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9467_length_1392_cov_2.005984"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1392
                     /locus_tag="PLH31_17600"
     CDS             <1..>1392
                     /locus_tag="PLH31_17600"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQR91044.1"
                     /translation="PRVAVAEPGANLTATIELLRAGHDRNVDLLLFPELGLSAYAIDD
                     LLLQDALLQGVDQALSDLVAASASVSPTLVVGAPLRRNGRLYNCAVAISRGRVLGVVP
                     KSFLPNYREFYENRWFAPGAGLTGLEIRVGGQVAPFGTDLIFEAEDLPGFTFHMELCE
                     DFWAATPPSSRAALAGALILCNLSASNIVVGKADDRELLCASQSMRCQAAYLYSAAGP
                     GESTTDLAWDGQATIHELGRRLAQTERFAAVSQMAVADVDVERLQLDRMRTPTFNNAA
                     VAAGHPEKDFRRVRFAHQPAFEDVGLVRPLDRFPFVPDDPARLDKDCYEAFNIQVQGL
                     TKRIQATKTQHIVIGVSGGLDSTHALIVAAKAFDAVGLPRSNILGFTMPGFATSDGTK
                     ENAWALMRSLGVTGEEIDIKPTARLMLQEIGHPFANGEPVYDITFENVQAGLRTDYLF
                     RLANQRHGFVLGTG"
BASE COUNT          221 a          487 c          448 g          236 t
ORIGIN      
        1 ccgcgcgttg cggtcgcgga gcccggcgcc aacctgaccg cgacaattga gttgttgagg
       61 gcagggcacg accggaatgt cgacctcctg ctgtttccag aactcggcct ctcggcctac
      121 gccatcgacg acttgctgct gcaagatgcc ctgctgcaag gcgtcgatca ggcgctgtcc
      181 gacttggtcg cagcttcggc gagcgtgtcg ccgacgctcg tggtcggagc gccgctacgt
      241 cgcaacggcc gcctctacaa ctgtgccgtc gccatctcta ggggacgggt tctgggcgtt
      301 gtgccgaaga gctttctgcc caactaccga gagttctacg agaatcgctg gttcgcgccg
      361 ggcgccggtt tgacggggct cgagatccgc gtcggcggac aggtcgcgcc gttcgggacc
      421 gatctgatct tcgaggccga ggatctgccg ggcttcacct tccatatgga actctgcgag
      481 gacttctggg cggcaacccc gccgtcgagc cgggccgcgc tcgcgggcgc gctgatcctc
      541 tgcaacctgt cggcctccaa catcgtggtc ggcaaggccg acgaccgcga gctgctctgc
      601 gcctcgcaat ccatgcgctg tcaggcggcc tatctttact ctgccgccgg gccgggggag
      661 agcaccaccg acctcgcctg ggacggccag gcgacgatcc acgagcttgg ccggcggctc
      721 gcgcaaacgg agcgttttgc ggctgtctcg cagatggcgg tcgccgacgt ggacgtcgag
      781 cggctgcagc tggatcgcat gcgaacaccc acgttcaaca acgccgcggt ggccgcgggc
      841 cacccggaga aggacttccg ccgggtgcgg ttcgcccacc agccggcgtt cgaggatgta
      901 ggcctggttc ggcccctgga tcgcttcccg ttcgtgcccg acgacccggc ccgcctcgac
      961 aaggactgct acgaggcctt caacatccag gtccagggcc tgaccaagcg tatccaggcc
     1021 accaagaccc agcacatcgt catcggcgtc tccggcggcc tggactcgac ccacgccctg
     1081 atcgtcgccg ccaaggcctt cgacgccgtg ggtctgccgc gcagcaacat cctgggcttc
     1141 accatgcccg gcttcgccac cagcgacggc accaaggaga acgcctgggc tctgatgcgc
     1201 agcctgggcg tcaccggcga ggagatcgac atcaagccga cggctcgctt gatgctgcag
     1261 gagatcggcc atcccttcgc caacggcgag ccggtctatg acatcacgtt cgagaacgtg
     1321 caggcggggc tcagaaccga ctacctgttc cgcttagcga accagcgcca cggcttcgtg
     1381 ctcggcaccg gc
//
LOCUS       DAOMEO010000989         1390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9482_length_1390_cov_2.286891, whole genome shotgun sequence.
ACCESSION   DAOMEO010000989 DAOMEO010000000
VERSION     DAOMEO010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_1390_cov_2.286891"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /gene="bamE"
                     /locus_tag="PLH31_17605"
     CDS             complement(<1..286)
                     /gene="bamE"
                     /locus_tag="PLH31_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HQR91045.1"
                     /translation="MFRPAVFVAAALGLSAVVSGCTPLTSYSGFQAIEARPADMKVGE
                     DSKSTLMEKLGSPSTISTFDPSTWYYVSQTSDRVAFYRPRVIKRDVIAIKF"
     gene            408..938
                     /locus_tag="PLH31_17610"
     CDS             408..938
                     /locus_tag="PLH31_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HQR91046.1"
                     /translation="MILDRLLKPRPAKAAGAKLYASAVAQARSAPLYAAMGVRDSLEG
                     RFELFTLHVVLLLERLKGQGDLAAETSQAVFDAYVRGLDDAFREIGVSDTAVGKKMKK
                     LAGAFYGRLKAFDEAAATLPDRTALGQMIGRTVLEERGEGDPEALTTYIIAVREHLAD
                     QSLDALLEGEAQWPNP"
     gene            923..>1390
                     /locus_tag="PLH31_17615"
     CDS             923..>1390
                     /locus_tag="PLH31_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQR91047.1"
                     /translation="MAEPVNTPWPCEVPLARVDRGDVSLKLEPDADQRKAIAKQLGLV
                     RLEEFSAEVYLSSWMDGAEVAGRLRARVIQLCSVSADDFEDSIEARFTVHVIPANSEH
                     APAEEGGELGLDPEADDPPDVLEGDVIDVSGYVLEHLALELDPFPRKPGAVFEN"
BASE COUNT          257 a          409 c          470 g          254 t
ORIGIN      
        1 tgaacttgat ggcgatgaca tcgcgcttga tcacgcgcgg cctgtagaac gccacccggt
       61 cagaggtctg ggacacatag taccaggtgc tggggtcgaa ggttgaaatg gtcgagggcg
      121 aacccagctt ctccatcaag gtggacttgc tgtcctcgcc taccttcatg tcagccgggc
      181 gggcctcgat cgcctggaaa ccggaatagc tcgtcaacgg cgtgcagccg gacactacgg
      241 cagacaggcc gagggcggcg gcgacaaaaa cggcgggacg aaacatgtag gctccggaac
      301 cggaaaaatg ctggactttg acctatcgcc cgcaggggct tagttcaatg gcccgcgtgg
      361 accggcagct catctagttc cgggtcgcgg cgaaatagag gcgttgaatg attctggatc
      421 gattgctcaa acccaggcca gccaaggcgg cgggggccaa gctctacgct tctgccgttg
      481 cccaggcgcg ttccgccccg ctttacgcgg cgatgggcgt tcgcgactca ctggagggcc
      541 ggttcgagct tttcaccctc cacgtcgtgc ttctgctgga acggttgaag gggcagggcg
      601 atcttgccgc tgaaacatcg caggccgttt tcgacgccta tgtgcgcgga ctggatgatg
      661 ctttccgcga gatcggcgtg tcagacacgg ccgtgggcaa gaagatgaag aagctggccg
      721 gcgccttcta cggccgcctg aaggcgttcg acgaggctgc ggccacgttg ccggaccgca
      781 ccgccctcgg ccagatgatc ggccgcacgg ttttggaaga gcgcggcgag ggcgatcctg
      841 aagcgctgac gacctatata atcgccgtgc gcgaacatct ggccgaccag tcgctggacg
      901 ccctgctgga aggagaagcg caatggccga acccgtaaac acgccctggc cctgcgaagt
      961 cccgctggcc cgcgttgatc gcggcgacgt cagcctgaag ctcgaacccg acgccgatca
     1021 gcgcaaggct atcgccaagc agttgggcct ggtgcgtctg gaggagtttt cggccgaggt
     1081 ttatctcagc tcgtggatgg acggcgccga ggtggcgggc cgtctacgcg cgcgggtgat
     1141 ccagctttgc agcgtgtcgg cggacgactt cgaggactcc atcgaggcgc ggttcacggt
     1201 ccatgtgatt ccggccaata gcgagcacgc gccggccgaa gagggcggcg agctgggcct
     1261 cgatcccgag gctgatgatc caccggacgt gctggagggt gatgtcatcg atgtctcggg
     1321 ctatgtcctg gaacatctgg cgcttgagct ggatccattt cctcgtaagc cgggcgcagt
     1381 gttcgagaat
//
LOCUS       DAOMEO010000990         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9492_length_1389_cov_2.363568, whole genome shotgun sequence.
ACCESSION   DAOMEO010000990 DAOMEO010000000
VERSION     DAOMEO010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_1389_cov_2.363568"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1389)
                     /locus_tag="PLH31_17620"
     CDS             complement(<1..>1389)
                     /locus_tag="PLH31_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQR91048.1"
                     /translation="DNLIETKATDVRDTLKNLGFAVLNRRLQALSRAGDPPFIAGGAF
                     SSQQYGAIRMTTLGATAQPGRWREALSALDQEQRRAAQYGVRQDELDREIASLRATLV
                     AAVSGEATQRTTQLANSIIGTLGDGETVTSPSQNLAAFDEIVKGLTADRVSAALKATF
                     SGQGPLILIPSPTNIEGGEKTVLEAYADSRKVAVLAPAAPGVTAWPYASFGPIGKVAE
                     QKDVTDLDAVFVRFENGVRLTVKPTKFRDDQVLIKARIGNGLLDLPPGAQSPLWSGSA
                     FIEGGLKQISAQDMERVLNGKIWNAALGVEDDAFTLSGRTRPEDLGTELQVLAAFAME
                     PGWRPEAFSRIKTYYGTIHDQLETTTGGVMGRDLSGLLHNGDRRWTFPSRADIAAASI
                     DNLKAAVTDPLSKGQIEVVVVGDITVDKAVAAVAETFGALPPRPGLADPTTTRLVPFP
                     APSATPVVLTHKG"
BASE COUNT          188 a          461 c          500 g          240 t
ORIGIN      
        1 cccttgtggg tcagcaccac cggcgtcgcg gacggcgccg ggaagggaac cagccgcgtc
       61 gttgtcgggt cggcgagacc cggacggggc ggcagcgcgc cgaaggtttc ggccacggcg
      121 gctacggcct tgtcgacggt gatatcgccc accaccacca cctcgatctg acccttgctg
      181 agcggatcgg tgacggcggc cttcaggtta tcgatcgagg cagcggcgat gtcggcgcgg
      241 gacgggaagg tccagcgcct gtcgccattg tgcagcaggc cgctgaggtc gcgacccatg
      301 acgccgccgg tggtggtttc cagctggtcg tggatggtgc cgtaataggt cttgatccgt
      361 gagaaggctt ctggccgcca gccgggctcc atggcgaagg ccgccagcac ctgcagttcc
      421 gtgccaaggt cctccggacg cgtgcggccg ctgagggtga aggcgtcgtc ctcgacgccc
      481 agggcggcgt tccagatctt gccgttcagc acccgctcca tgtcctgggc gctgatctgc
      541 ttgaggccgc cctcgatgaa ggccgagcct gaccacagcg ggctctgggc gccgggcggc
      601 agatcgagca ggccgttgcc gatgcgagcc ttgatcagca cctggtcgtc gcggaacttg
      661 gtcggcttga ccgtcaggcg cacgccgttc tcgaagcgca cgaagacggc gtccagatcg
      721 gtgacgtcct tctgttcggc caccttgccg atggggccaa agctggcata gggccaggcc
      781 gttacgcccg gcgcggccgg ggccaggaca gcgaccttgc gggagtcggc ataggcctcc
      841 agcaccgtct tctcgccccc ctcgatattg gtggggctgg ggatcaggat cagcgggccc
      901 tggccgctaa aggtcgcctt gagcgccgcg ctgacccggt cggcggtcag gcccttcacg
      961 atctcgtcga aggcggccag gttctggctg gggctggtca cggtctcgcc gtcgcccagg
     1021 gttccgatga tgctgttggc cagctgcgtg gtgcgctggg tggcctcgcc cgagaccgcc
     1081 gcgaccaaag tggcgcgcag gctggcgatc tcgcggtcca gctcgtcctg acgcacgcca
     1141 tattgcgcgg cgcggcgctg ttcctgatcc agcgccgaca gggcctcgcg ccagcgaccc
     1201 ggctgggctg tcgcgcccag ggtcgtcatg cgtatggcgc cgtactgctg gctgctgaac
     1261 gccccgccgg cgatgaacgg cgggtcgccc gcgcggctca gggcctgcag gcgtcggttc
     1321 agcaccgcaa agcccaggtt cttcagcgtg tcacggacgt cggtggcctt ggtctcgatc
     1381 aggttgtcg
//
LOCUS       DAOMEO010000991         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9497_length_1389_cov_1.714393, whole genome shotgun sequence.
ACCESSION   DAOMEO010000991 DAOMEO010000000
VERSION     DAOMEO010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9497_length_1389_cov_1.714393"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>325)
                     /locus_tag="PLH31_17625"
     CDS             complement(<1..>325)
                     /locus_tag="PLH31_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HQR91049.1"
                     /translation="AIGVAVGLARENHGAAGLAGVVCYVITAKGVDALMVVPPEIAAA
                     AVESARDLTIAAWKAKEIGKLSIPVGILSGVISGWLYNRYSNIKLPEYLAFFGGRRFV
                     PIVAGL"
     assembly_gap    326..335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<336..517)
                     /locus_tag="PLH31_17630"
     CDS             complement(<336..517)
                     /locus_tag="PLH31_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl-D-glucosamine transporter"
                     /protein_id="HQR91050.1"
                     /translation="MKSPLEVLQPLGRALMLPIAVLPVAALLLRIGQPDLLGAPGLAA
                     MTGGLSLHIANVFGAAG"
     gene            complement(521..>1389)
                     /locus_tag="PLH31_17635"
     CDS             complement(521..>1389)
                     /locus_tag="PLH31_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQR91051.1"
                     /translation="DALAASANGAEGSGLLRTEFLFLDRATPPDEDEQARQYQAIASA
                     LDGKPLIIRTPDVGGDKAAPYLPIPAEENPALGLRGVRVSLWRPHLLKTQIRAILRVR
                     PIGQCKIMVPMIASLDELRAVRAVLDEAKREMGISERIELGVMIETPAAAVTADLIAA
                     EADFLSIGTNDLTQYVLAMDRGNPELAARIDALHPAVLRMIAQTCAGAAKHHRWVGVC
                     GGAASDLAAVPILLGLGVTELSATAAVVPEVKALVRALDLEACRTLAAQVLNLPSPEA
                     VREHAKAFVAGV"
BASE COUNT          228 a          454 c          493 g          204 t
ORIGIN      
        1 agacccgcga cgatcggcac gaagcggcgt ccgccaaaga aggccaggta ctcgggcagc
       61 ttgatgttgg aatagcggtt gtagagccag ccgctgatca cccctgacag gatgccgacc
      121 gggatcgaca gcttgccgat ctccttggcc ttccacgccg cgattgtcag gtcccgcgcg
      181 ctctcaacgg ctgcggcggc gatctcaggc ggcacgacca tcagggcgtc cacgcccttg
      241 gcggtgatca cgtagcagac gacgccggcg agccccgccg cgccatggtt ctcgcgcgcc
      301 agaccgacgg cgacaccgat ggcgannnnn nnnnncctgc tgcgccaaac acattggcga
      361 tgtgcagcga caggccgccc gtcatggcgg ccaggccggg cgcaccaagc aagtcgggtt
      421 ggccaatccg cagcaacagc gccgccactg gcagcacggc gatgggcagc atcagggcgc
      481 ggcccagggg ctggaggact tcgagcggcg acttcatcgc ttagactccg gccacaaagg
      541 ctttggcgtg ttcacggacc gcctcgggcg agggtagatt cagcacctgg gcggccaggg
      601 tgcgacaggc ctcaaggtcg agcgcgcgga ccagagcctt cacctcgggc accacggcgg
      661 cggtggctga gagctcggtg acgcccaggc ccagcaagat cggcacggcg gccagatcgg
      721 acgccgcgcc gccgcagacc ccgacccagc gatggtgttt ggccgcgccg gcgcaggtct
      781 gggcgatcat ccgcagcaca gccgggtgca gggcgtcgat gcgggcggcg agctccggat
      841 ttccccggtc catggccagc acatactggg tcaggtcatt ggtgccgatg gacaggaagt
      901 cggcctcggc ggcgatcagg tcggcggtga cggcggcggc gggcgtctcg atcatcacgc
      961 ccagctcgat gcgctcggat atccccatct cgcgcttggc ttcgtcgagc acggcgcgga
     1021 cggcgcgcag ttcgtcgagg ctggcgatca tcggaaccat tatcttgcac tgaccaatag
     1081 gacgcacacg gagtattgct cggatctggg tcttcagcag atgcggtcgc cacagcgaaa
     1141 cccgcacgcc gcgcagacca agcgccggat tttcctcggc cgggatcggc aggtagggcg
     1201 cggccttgtc gccgccgacg tcgggggtgc ggatgatcag cggcttgccg tccagggccg
     1261 aggcgatggc ctggtactgg cgggcctgtt catcctcgtc gggcggggtg gcgcggtcga
     1321 ggaacaggaa ttcggtgcgc agcaggccgc tgccttccgc gccgttggcc gaggcggcca
     1381 gggcgtcat
//
LOCUS       DAOMEO010000992         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9501_length_1388_cov_3.201050, whole genome shotgun sequence.
ACCESSION   DAOMEO010000992 DAOMEO010000000
VERSION     DAOMEO010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9501_length_1388_cov_3.201050"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="hfaD"
                     /locus_tag="PLH31_17640"
     CDS             <1..1112
                     /gene="hfaD"
                     /locus_tag="PLH31_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holdfast anchor protein HfaD"
                     /protein_id="HQR91052.1"
                     /translation="VFAEQTLNVETVTDGFSASTIAPANSALISVQNTDASMTSNQEN
                     SGADLAHGVVNVATHGGNNSNFTTTAVGNAGTAAADNGTLTGRATQLNTGPVTARSQL
                     EAHEAELNDMSMSTLAQGNSQGLTLINGAMGVRVSQGNTANVLADGGAIAGAITGTAA
                     VAGTATGNNITLSGTEQSAARVITEQSNAGITQASKFTAYGSSYLSSTAASASGNNLS
                     ATNEGPLLDVASSQYNTAYVRAQAEATAYQFSAAQVGAHGVGNSALVGNYGPEVVLDN
                     IQTNEGGGVEVLATFEGYDGYDAVASSTAMGNAVTGYACSQCSGQMTIKNNQVNSAEI
                     GARTSTIAGSARSVSGITTATGNNASFYVSSPGGN"
     gene            complement(1113..>1388)
                     /locus_tag="PLH31_17645"
     CDS             complement(1113..>1388)
                     /locus_tag="PLH31_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR91053.1"
                     /translation="AVQSRDYDLVLMDVRMPVMDGLTATRAIRALKTDMARIPIIALT
                     ANVLADQVASYLAQGMDDHVGKPIDPQDLLLKIARWSDLEVGRGSHH"
BASE COUNT          284 a          476 c          425 g          203 t
ORIGIN      
        1 acgtctttgc cgagcaaacc ctgaacgtcg aaaccgtcac cgacggcttc agcgccagca
       61 ccatagcgcc ggccaacagc gccctgatct cggtgcagaa caccgacgcc tcgatgacct
      121 ccaaccagga aaattccggc gcggatctgg cgcatggcgt cgttaatgtc gcgacacatg
      181 gcggcaataa cagcaacttc accaccacgg cggtcggcaa tgccggcaca gccgcagccg
      241 acaacggcac cctgacgggc cgcgccacac agctgaacac cggaccggtc accgcgcgca
      301 gtcagcttga ggctcacgaa gccgagctga atgacatgtc gatgagcacc ctggcccagg
      361 gcaacagcca gggcctgacc ctgatcaatg gcgccatggg cgtgcgggtc agccagggca
      421 atacggccaa cgtcttggcc gacggcggcg cgatcgccgg cgcgatcacc ggcacggccg
      481 ccgtcgcggg cacggcgacg ggcaacaaca tcaccctgtc ggggaccgag cagtcggcgg
      541 cccgtgtgat taccgagcag tcaaacgccg gcatcaccca ggccagcaag ttcacggcct
      601 atggctccag ctatctgagc agcacggcag cctcggccag cggcaacaat ctgtcagcga
      661 ccaatgaagg cccgctgctg gacgtggctt caagccagta caacaccgcc tatgtgcgcg
      721 cccaggccga ggcgacggcc taccagttca gcgccgctca ggttggcgcc catggtgtcg
      781 gcaattccgc cctggtgggc aactatggtc ccgaggtcgt gctcgacaac attcagacga
      841 atgaaggcgg cggagtcgag gtgctggcga ctttcgaagg ctatgacggc tatgacgccg
      901 tggcgtcttc cacggccatg ggcaatgccg tcaccggcta tgcctgctcg cagtgcagcg
      961 gccagatgac catcaagaac aaccaggtca acagcgccga gatcggcgcc cgcaccagca
     1021 ccatcgcggg ctccgcacgt tcggtcagcg ggatcaccac cgccaccggc aacaacgcca
     1081 gcttctatgt gagctcgccc ggcggcaact agtcagtggt gcgagccgcg ccccacctcc
     1141 aggtcgctcc atctggcgat cttcagcaac aggtcctgcg ggtcgatcgg cttgccgacg
     1201 tgatcatcca tgccctgggc gagataggac gccacctgat cggccagcac attggccgtc
     1261 agggcgatga tgggaattcg ggccatatcg gttttcaggg cccgaatggc ccgggtggcg
     1321 gtcaggccgt ccatcaccgg catccgcaca tccataagga caaggtcgta gtcgcgcgac
     1381 tgcaccgc
//
LOCUS       DAOMEO010000993         1387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9507_length_1387_cov_4.158408, whole genome shotgun sequence.
ACCESSION   DAOMEO010000993 DAOMEO010000000
VERSION     DAOMEO010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9507_length_1387_cov_4.158408"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1387)
                     /locus_tag="PLH31_17650"
                     /pseudo
     CDS             complement(146..1387)
                     /locus_tag="PLH31_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          237 a          456 c          462 g          232 t
ORIGIN      
        1 tcaaacgatt gatccggcaa agctcgtcgg gccgatgttg gcccggttca tgtctgctga
       61 tcgagcgttc gctaaagcgc ggcgttcggc aaaagagccg gcgcagaaag tggatgctag
      121 gtgaggatga tcggatagtg cgtttgtagt ccatcgcgcc ggcgacgtcg ccgtgtcgag
      181 acagcttgcg ccgctcatca cgcatccagg cttccagtgc gtagaccagt ggcgcgctgc
      241 gttcccgacg aaccgccagc cgctgctcgg cgccggcaga ccgttgatgt cgcgctcaat
      301 atccaacagc gcgtcgatcc gctggaccgc ttccagggcg atcggcgaga ggacctggag
      361 tttgcgacct tgggcgcggc gtcgcacacc ggcctcgata tcggcgagga cgaagaactt
      421 gcgccgagcg tgggcccagc aggcggcctc gatgagtctc ggccgtcgca cgacgtcgta
      481 cagcttgccg taaccactgt aggcgtcagt ctgcaggatg ccgccccaat cggccaggcg
      541 ctgctgggga tgctcgccga accggtcgcg cgagtagaag aacaccgcag ccggcggtgt
      601 ccgcccaccg aacggccggt catcgcgcac ataggtccca catcgcccga tgatggtctt
      661 gcccctggcc agcaccggca ccgtggtgtc gtcgccaagc agacggtcgg cggccaggac
      721 gtaagccaga aggcgatcat ggacgggccg cagcaccgtg cagcacgccc caatctgatc
      781 ggccagcgtg gagagactca acggcacacc ctcgggcgcg tagcgctcgg cctggcggtt
      841 gagatgctga tgctggcctt acttctcgaa caggatcatc gccagaaggc tggggccggc
      901 ccagcctctg ggcacgacgt ggaaacggca ccggcgactg gttgatgctc tcgcagtcgc
      961 ggcccgagaa cttctcgcgc acatgccgga tcaccttcca ctgacggggg atgacctcca
     1021 gggtctcggt gacgtcctcg cccagcttcg acaggcgcgc accgccacag cagcgacagg
     1081 cggtcggtcc gggaacgatc acccgctcgc gcggtagatg ttcggggaag ggcttgcgcg
     1141 ctggccgctg ccgggtgaag gccgccacct tggtggtcct ggcggccact cgctctgcca
     1201 ccagttcgtc ctccgtcgcc gaggcctcga gctcctcgag ctggagctca agctggtgga
     1261 gcagccggga actgcgttcg gagcgctggc caaaccgctc gcggcggagc ttcccaatct
     1321 gcagcttcag atggacgatc aacgcctgat cgtcggctgc cttggctcgc aagcttgctg
     1381 cttaggc
//
LOCUS       DAOMEO010000994         1383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9538_length_1383_cov_2.708584, whole genome shotgun sequence.
ACCESSION   DAOMEO010000994 DAOMEO010000000
VERSION     DAOMEO010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_1383_cov_2.708584"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLH31_17655"
     CDS             <1..274
                     /locus_tag="PLH31_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQR91054.1"
                     /translation="LLASAHHGLTHRIDPGPAVVGDGYAAAAKEKSRLPSNWFSAVDL
                     FDQSTVPRDYLGDRFVDMFVSVKRTEQARFFEVVTSLDFDWYLRNA"
     gene            355..>1383
                     /locus_tag="PLH31_17660"
     CDS             355..>1383
                     /locus_tag="PLH31_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR91055.1"
                     /translation="MGTLTQRGLNRRSLLAATGAAAVGLTFTACGQKPAAPTGAEEAK
                     LNFYNWDTYIGDTTLADFKTATGVDVNMSLFATNDELFAKLKAGNPGFDVIVPSNEFV
                     TRMGQAGLLEVLDHSKIPNLKNVDQAFMNPEYDPGRKYSMPYTWLVLGIGYRKSKVKG
                     VPDSWKYLFDSNQYAGKIALLSESADLIRLSAKYKGHSVNNIPPEMVAEIEAMLIKQK
                     PFIKAFHDDNGQDMLASGEVDLVLEYNGDIAQLMKEDADVDFVIPKEGSLINSDTLCI
                     PKGAPRPDNAHKFINYLLDAQAGAAISKTILYPTPNAAAKALMPEDYKNNPVIFPPPE
                     VMAKCEYGA"
BASE COUNT          292 a          431 c          426 g          234 t
ORIGIN      
        1 gctgctggcc agcgcccatc acggcctgac ccacaggatc gatccgggcc cggcggtggt
       61 gggcgacggc tatgcggccg ccgccaagga gaagtcgcgc ctgccctcca actggttttc
      121 ggcggtcgac ctgtttgatc agtccacggt gccgcgcgac tatctgggtg accgctttgt
      181 ggacatgttc gtgtcggtga agcggaccga acaggcgagg ttcttcgagg tggtcacatc
      241 cctcgatttc gattggtatc tccgcaacgc ctgacggcgg agtggagcgg ggcttgcgtt
      301 gaaccgccgg cgcaagccgg catcgaacga aacgaacagg ggagtttcga aaccatgggt
      361 acgctcacgc aacgtggact gaatcgccgg tcgctgctgg cggcgacggg ggccgccgct
      421 gtcggcctga ccttcaccgc ctgcggccag aagccggccg cgccgacggg ggccgaggaa
      481 gccaagctga atttctacaa ctgggacacc tatatcggcg acaccaccct ggccgacttc
      541 aagacggcca ccggcgtcga cgtgaacatg agcctgttcg ccaccaatga cgagctgttc
      601 gccaagctga aggcgggcaa tccgggcttt gacgtgatcg tgccgtccaa tgaattcgtt
      661 acccgcatgg gccaggccgg cctgctggaa gtgctggacc acagcaagat tcccaacctg
      721 aagaacgtcg atcaggcctt catgaacccg gagtatgacc cgggccgcaa atattcaatg
      781 ccctatacct ggctggttct gggcatcggc tatcgcaaga gcaaggtgaa gggcgttcct
      841 gacagctgga agtatctgtt cgacagcaac cagtacgccg gcaagatcgc cctgctgtcg
      901 gaaagcgccg acctgatccg cctgtcggcc aagtacaagg gccacagcgt caacaacatc
      961 ccgcctgaaa tggtcgcaga gatcgaggcg atgctgatca agcagaagcc cttcatcaag
     1021 gcgttccacg acgacaatgg tcaggacatg ctggcctcgg gcgaggtgga tctggtgctg
     1081 gaatacaacg gcgacatcgc ccagctgatg aaggaagacg cggacgtcga cttcgtgatc
     1141 cccaaggaag gatcgctgat caactccgac accctgtgca ttcccaaggg tgcgccgcgt
     1201 ccggacaacg cccacaagtt catcaactat ctgctggacg cccaggccgg ggcggcgatc
     1261 tccaagacca tcctgtatcc gacgcccaac gccgcggcca aggcgctgat gccggaagac
     1321 tacaagaaca atccggtgat cttcccgccc cccgaggtga tggccaagtg cgaatacggc
     1381 gcc
//
LOCUS       DAOMEO010000995         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9550_length_1382_cov_2.591560, whole genome shotgun sequence.
ACCESSION   DAOMEO010000995 DAOMEO010000000
VERSION     DAOMEO010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9550_length_1382_cov_2.591560"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLH31_17665"
     CDS             complement(<1..705)
                     /locus_tag="PLH31_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91056.1"
                     /translation="MHIFSRARVVLMTAAVALTTVVAGCETPPAPPPVVAAPPAPPPI
                     TLSNALVERASAYRGYMARAGAIDPKFQNGDQIQSSLKVGVAYEPKQLLSGVTAYAAV
                     IALQDPAFVGAVRSFAADPTQRQQVIAQLVADPAYAVGFKGSDTAAGLVIDTLGAEGL
                     KVYTAGKAVKQAAYDVQHSSWSKASVQDRDGRLAYAKTMSAIPALGDTSDVAALQQAS
                     VGAQPLSLTPRSASGPY"
     gene            complement(843..>1382)
                     /gene="prs"
                     /locus_tag="PLH31_17670"
     CDS             complement(843..>1382)
                     /gene="prs"
                     /locus_tag="PLH31_17670"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HQR91057.1"
                     /translation="FFDIPTDNLLPSRLLADDIIRNYSLGDDLMVVSPDVGGVVRARA
                     LAKRLNDADLAIVDKRRSGPGQSEVMNIIGDVSGRRCILFDDIADSAGTLCNAAAALI
                     ANGATSVSAYVTHGVLSGAAAERVAGSVLTELVVTDSIEASDPVKACPKIRYVSCAPL
                     IGEAIRRIANEESVSKLFD"
BASE COUNT          229 a          425 c          517 g          211 t
ORIGIN      
        1 ataggggccc gaagccgaac gcggcgtcag gctgaggggc tgggcgccga ccgaggcctg
       61 ctgcagggcg gccacgtcgc tggtgtcgcc cagggccggg atcgccgaca tggtcttggc
      121 ataggccaga cgaccatcgc gatcctgcac cgaggccttg gaccaggacg agtgctgaac
      181 gtcataggcc gcctgtttga cggccttgcc ggcggtatag accttcaggc cttccgcgcc
      241 gagcgtgtcg atgaccaggc cggcggcggt gtcgctgccc ttgaagccga cggcataggc
      301 cgggtcggcc acaagctggg cgatgacctg ctggcgctgg gtcggatcgg ccgcgaagct
      361 gcgcaccgcg ccgacgaagg cggggtcctg cagggcgatg acggcggcat aggcggtgac
      421 gccggacagc agctgcttgg gctcgtaggc gacgcccacc ttcagcgagg actggatctg
      481 atcgccgttc tggaacttgg gatcgatcgc gccggcccgg gccatgtagc cccgataggc
      541 gctggcccgc tcgaccagag cgttggacag ggtgatcggc ggcggcgctg gcggcgcggc
      601 gaccaccggc ggcggcgcag gcggggtttc gcagccggcc acgacagtgg tcagtgcgac
      661 ggcggcggtc attagaacga cgcgcgcacg cgaaaagatg tgcattcggg cgaacttccc
      721 catgggtctg attttcgcga atagcatccg cgacgggcga tattgtgaca ctcagcccgt
      781 taaccagact ttgacaagcg tggccgcaag cgccttcgcc cctggggaga gattgccggg
      841 gatcagtcga acagcttgga gaccgattcc tcgttggcga tacggcggat cgcttcgccg
      901 atcagcgggg cgcaggagac gtagcggatc ttggggcagg ccttgacggg atctgaggcc
      961 tctatggaat cggtgaccac cagctcggtc aggactgacc cggcgacgcg ctcggcggcc
     1021 gcgcccgaca gcaccccgtg ggtgacatag gcgctgaccg aggtggcgcc attggcgatc
     1081 agggcggcgg cggcgttgca cagggtgccg gccgagtcgg cgatgtcgtc aaacaggatg
     1141 cagcggcggc ccgagacgtc gccgatgatg ttcatcactt ccgactggcc agggcccgag
     1201 cggcgcttgt cgacgatggc cagatcggcg tcgttcaggc gcttggccag ggcccgggcg
     1261 cgcaccacgc cgcccacgtc gggcgagacc accatcaggt cgtcgcccaa cgagtagttg
     1321 cggatgatgt cgtcggccag caggcgcgag ggcagcaggt tgtcggtcgg gatgtcgaag
     1381 aa
//
LOCUS       DAOMEO010000996         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9552_length_1382_cov_2.181613, whole genome shotgun sequence.
ACCESSION   DAOMEO010000996 DAOMEO010000000
VERSION     DAOMEO010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9552_length_1382_cov_2.181613"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLH31_17675"
     CDS             <1..481
                     /locus_tag="PLH31_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HQR91058.1"
                     /translation="GVLPPVCENGAVLVDGAVMKNFPADVMRAFQLGPIVGVDVTRGR
                     SITDEDVVTPPSLWRWFWSGEWRKGPPIVALLMRAATVSTGRDLAASREASDVLITPR
                     MDKIDIRDWRAFAPAVAAGKIAAAFALDGLDRPVNDLRRRPSLAERARAALTPSGRG"
     gene            complement(459..1250)
                     /locus_tag="PLH31_17680"
     CDS             complement(459..1250)
                     /locus_tag="PLH31_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQR91059.1"
                     /translation="MKVQSMPDWNPDIYALYQAYRERPALDLLTAIPRDLNPGEIWDL
                     GCGAGEQAALLAARHPGATVHGLDSSPEMLERARGLGARVGWVQGDIASFAPAGPVDL
                     LFTNAALQWVDDHPALFPRLAGALAPRGVLACQMPLVADVAWRKTLGEIVATGPWAAR
                     LAGVQGVRPSHEAGDYYDWLAPLCPEIDIWTTTYLHALHGQDPIVDWTMGTTLLPFLE
                     ALRDTEEKAAFLDAWRARLARDYPRRADGVTLFPFTRLFILARRG"
BASE COUNT          226 a          486 c          463 g          207 t
ORIGIN      
        1 cggcgtgctg ccgcctgtgt gcgagaatgg tgcggttctg gtcgatgggg cggtgatgaa
       61 gaacttcccg gccgacgtca tgcgcgcctt tcagctgggg ccgatcgtcg gcgtggatgt
      121 cacccgcggc cgcagcataa ccgacgagga cgtggtcacg ccgccgtccc tgtggcgatg
      181 gttctggtcg ggcgaatggc gcaaggggcc gcctatcgtg gccctgctga tgcgcgccgc
      241 caccgtctcg acaggacgcg atctcgccgc cagtcgcgaa gcctcggatg tgctgatcac
      301 cccccggatg gacaagatcg acatccgcga ctggcgcgct ttcgcccctg cggtggcggc
      361 cggcaagatc gccgccgcct tcgccctcga tggcctggac cggcccgtca atgacctgcg
      421 ccgccggccc agccttgccg aacgcgcccg cgcggcgctc accccctccg ggcgaggatg
      481 aacagccggg tgaagggaaa cagggtgacg ccgtccgccc ggcgtggata gtcacgcgcc
      541 agccgcgccc gccaggcgtc aagaaaggcc gccttctcct ccgtatcgcg cagggcctca
      601 aggaacggca gcaaggtcgt gcccatggtc cagtcgacga tggggtcctg cccatgcagg
      661 gcgtgcagat aggtcgtcgt ccagatatcg atctccggac acagcggcgc cagccagtcg
      721 tagtagtcgc cagcctcgtg agagggacgc acgccctgga cgccggccag gcgcgcggcc
      781 cacggccccg tcgcgacgat ctcgccaagg gtctttcgcc aggccacatc cgccaccagc
      841 ggcatctggc aggccagcac gccgcgcggc gccagagcgc ccgccagacg cgggaacagc
      901 gcagggtgat cgtcgaccca ctgcaaggcg gcattggtga acagcagatc gaccggccct
      961 gcgggcgcaa agctggcgat gtcgccctgg acccagccca cccgcgcgcc aagccccctc
     1021 gcccgctcca gcatctccgg gctggagtcc aggccatgca ccgtcgcccc ggggtggcgc
     1081 gccgccagca gcgcggcctg ttcgccagcg ccgcagccca gatcccagat ctcgcccggg
     1141 ttcaggtccc gcgggatcgc cgtcaaaagg tcgagcgcgg ggcgctcccg ataggcctgg
     1201 tagagcgcat agatgtcggg gttccagtcg ggcatggatt ggaccttcac tcggggtgcg
     1261 acaatcggcg caagctgtag cgcgccagaa ccctcttaac ctctttgtta agatgcgatt
     1321 caccctatag ttaagtcagg tctccgacag agagactttt gggaaggaag ctcgatgcct
     1381 cg
//
LOCUS       DAOMEO010000997         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9564_length_1381_cov_3.130468, whole genome shotgun sequence.
ACCESSION   DAOMEO010000997 DAOMEO010000000
VERSION     DAOMEO010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9564_length_1381_cov_3.130468"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLH31_17685"
     CDS             complement(<1..589)
                     /locus_tag="PLH31_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR91060.1"
                     /translation="MSQPMIVDVVADVVCPWCYLGWRRVKAAVALRPDIEAKLVWRPY
                     QLDPTISEQGVDRKAYMAAKFKDSERLRAVHDALVTGGADDGITFNFDIIEKSPNTNA
                     AHRLIRWALTAGVQDAVVEALFAAYFTQGRDIGDPMVLADIAEAAGMERLVVLQLLSE
                     GADKESVTREHSMAVQGGVTGVPYVIFGGKVAVVGA"
     gene            complement(598..>1381)
                     /locus_tag="PLH31_17690"
     CDS             complement(598..>1381)
                     /locus_tag="PLH31_17690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQR91061.1"
                     /translation="AYTAFDYLHERAFDSVVLWAGDNQQEALSIAAGMRRNTRLFHIP
                     AMLYLKAESYVTMSEAFHRGVSDVASPETAEVETARRVMELARNYRRGESIRGALEKA
                     RSSGLMDAATGLFTRDLFAAHLARLTAAARERSRPLSICVLRVADKPETVWARQNGWL
                     DRAIPQIGSMVGRLVRVEDTAARLAPEVFALALPATNQNAARAAAERIAAVIGCTAFD
                     AGEDRAPFVCEFDIGVAQIEAGEGAVKALERAAARALQREAS"
BASE COUNT          223 a          471 c          477 g          210 t
ORIGIN      
        1 ccgccccgac gaccgcgacc ttgccgccga agatcacata gggcacgccg gtgacgccgc
       61 cctgcaccgc catggagtgc tcgcgggtca ccgactcctt gtcggcgcct tcggacagca
      121 gttgcagcac gaccaggcgc tccatgcccg ccgcctcggc gatatcggcc agcaccatgg
      181 gatcgccgat gtcgcgacct tgtgtgaagt aggccgcgaa cagcgcctca accacggcgt
      241 cctggacgcc ggcggtcagc gcccagcgga tcagtcggtg cgcggcgttg gtgttgggcg
      301 acttttcgat gatgtcgaag ttgaaggtga tcccgtcatc ggcgccgccc gtgaccagag
      361 cgtcgtgcac cgctcgcagc cgttccgagt ccttgaactt ggccgccata taggctttgc
      421 gatcaacgcc ttgctcggaa atcgtcgggt ccagctgata gggccgccag accagcttgg
      481 cctcgatatc cgggcgcagg gccacggccg ccttcacgcg tcgccagccc agatagcacc
      541 acggacacac aacgtcggcg accacgtcca cgatcatcgg ctggctcatc gggggcctca
      601 gctggcttcg cgttgcagcg cccgggcggc ggcgcgttcg agggccttga ccgccccttc
      661 gccggcttcg atctgggcga caccgatgtc gaactcgcag acgaagggcg cacggtcctc
      721 gccggcgtca aaggcggtgc agccgatgac ggcggcgatg cgctcggcgg cggcgcgggc
      781 ggcgttctgg ttggtggcgg gcagggccag ggcgaagacc tctggcgcca ggcgcgcggc
      841 ggtgtcctcg acccggacca ggcgacccac catcgagccg atctggggaa tggcgcgatc
      901 cagccagccg ttctgacggg cccagacggt ctccggcttg tcggcgaccc gcagcacgca
      961 gatcgacagc ggacgcgagc gctcacgggc ggcggccgtc agacgcgcca gatgggcggc
     1021 gaacaggtcg cgggtgaaga gacccgtggc ggcgtccatc aggcccgaac tacgcgcctt
     1081 ttccaacgcg ccccggatgg attcgccccg gcgatagttg cgggccagtt ccatcacacg
     1141 gcgcgcggtc tcgacctcgg cggtctcggg cgaagcgacg tcggaaacgc cccgatggaa
     1201 agcctccgac atggtcacat agctctcggc cttcagatac agcatggccg ggatgtggaa
     1261 gaggcgggtg ttgcggcgca tgccggccgc gatcgacagg gcttcctgct gattgtcgcc
     1321 ggcccacagc accacggaat cgaaggcccg ctcgtgcagg tagtcgaagg ctgtgtaggc
     1381 g
//
LOCUS       DAOMEO010000998         1381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9566_length_1381_cov_2.458522, whole genome shotgun sequence.
ACCESSION   DAOMEO010000998 DAOMEO010000000
VERSION     DAOMEO010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_1381_cov_2.458522"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1142)
                     /locus_tag="PLH31_17695"
     CDS             complement(<1..>1142)
                     /locus_tag="PLH31_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQR91062.1"
                     /translation="GVTNQVVLLIATVMLLGLFFLQSRGTATVGKLFGPVCAVWFSVM
                     FLLGAVNLAQNPGVLMAISPHYAVGFLADHGLTGFIVLGAVFLTVTGVEALTADMGHF
                     GRWPIQAAWLFFVLPCLTMNYLGQGAFALTTLNAAEAAGQPMPELNWFFEMAPAVLRL
                     PLVLLAGAATVIASQAVITGAFSLTQQAIQLGLLPRIDIRRTSETQAGQIYVPQVNTI
                     LLFGVLAIMFTFQTSSALAHAYGLAVTGTMIVTTCMAFIVMRRLWNWSLPMALCFLVP
                     FLTLDVVFLTANALRFFSGGWLPVLIGAVLFLVMATWVRGSQILTAKTRRDSVPLVDL
                     MEILRARAPHRAPGTAIFLTSDPDMTPVALMHNLKHNKVLHERNVI"
     assembly_gap    1143..1152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1153..>1381)
                     /locus_tag="PLH31_17700"
     CDS             complement(<1153..>1381)
                     /locus_tag="PLH31_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQR91063.1"
                     /translation="WALILVVTIKYVVFIMRADNKGEGGVLSLMALAQHAMGKRTVLV
                     FALGVAGAALFYGDALITPAMSVLSAVEGLRT"
BASE COUNT          273 a          423 c          483 g          192 t
ORIGIN      
        1 ggatcacatt gcgctcgtgc agaaccttgt tgtgcttgag gttgtgcatc agcgccacgg
       61 gcgtcatgtc cgggtcggag gtcaggaaga tcgccgtgcc gggggcgcgg tgcggcgcgc
      121 gggcccgcag gatttccatc aggtcgacca gggggacgct gtcgcggcgg gtcttggcgg
      181 tgagaatctg cgatccgcgc acccaggtgg ccatgaccag gaacagcacc gcgccgatca
      241 gcaccggcag ccaaccgccc gagaagaagc gcagggcgtt ggcggtcagg aacaccacgt
      301 ccagggtcag gaagggcacg aggaagcaca gggccatcgg caggctccag ttccacagcc
      361 ggcgcatgac gatgaaggcc atgcaggtgg tcacgatcat ggtgccggtc accgccaggc
      421 cataggcgtg ggccagggcc gacgaggtct ggaaggtgaa catgatcgca agcacgccaa
      481 acagcaggat ggtgttgacc tggggcacat agatctggcc ggcctgggtc tcgctggtgc
      541 ggcggatgtc gatgcgcggc agcaggccca gctggatggc ctgctgggtc agggagaagg
      601 cgccggtgat caccgcctgg ctggcgatga ccgtggctgc gccggccagc agcactagcg
      661 gcaggcggag cacggccgga gccatttcga agaaccagtt cagttccggc atcggctggc
      721 cggcggcctc ggcggcgttg agggttgtga gggcgaaggc gccctggccc agatagttca
      781 tggtcaggca gggcaggacg aagaacagcc aggccgcctg aattggccag cggccaaaat
      841 ggcccatgtc ggcggtcagg gcctcgacgc cggtcacggt caggaacacc gcgcccagca
      901 cgataaagcc cgtcaggccg tgatcggcca ggaagcccac ggcatagtgc gggctgatcg
      961 ccatcagcac gccgggattc tgggccagat tgacggcgcc cagcaggaac atcacgctga
     1021 accacacggc gcagactgga ccaaacagct tgcccaccgt cgctgtgccg cggctctgca
     1081 ggaagaacag gcccagcagc attacggtgg cgatcagcag tacgacctgg ttggtgacac
     1141 ccnnnnnnnn nnggttcgca gaccctcgac cgccgacaag accgacatgg ccggggtaat
     1201 caaggcgtcg ccgtaaaaca aggctgcgcc agccacgccc agggcgaaca ccagcacggt
     1261 gcgcttgccc atggcgtgtt gcgccagggc catcagcgac agcacgccgc cttcgccctt
     1321 gttgtcggcg cgcatgatga agacgacgta tttgatcgtc acgaccagga tcagcgccca
     1381 c
//
LOCUS       DAOMEO010000999         1379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9582_length_1379_cov_2.777190, whole genome shotgun sequence.
ACCESSION   DAOMEO010000999 DAOMEO010000000
VERSION     DAOMEO010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_1379_cov_2.777190"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>917
                     /locus_tag="PLH31_17705"
     CDS             79..>917
                     /locus_tag="PLH31_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR91064.1"
                     /translation="MGNQTVAISTADASDPGHAQDFFDLAEALLNLAAGQYGVRAPPS
                     SHPSAEHDAFNRLAEHLDKASSADLPASLLTLNPRPSDAAGLSYIAILGLDGFSRLRR
                     QIGSQVASQILKTLGERVRLLLPGVKLGRIGRTNLEFAFRAADDLEAVSLLSALRLEL
                     DRRLDLADGQSFDLDVAFGAARHDRCGEFVIDCAAEALVQAQSQATKVQIYREEDRER
                     AAASLALLRDLREAIAQDGLALAYQPKLRLSSDRMEVAEALVRWNHPVRGAVSPDEFI
                     GLA"
     assembly_gap    918..950
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <951..>1379
                     /locus_tag="PLH31_17710"
     CDS             <951..>1379
                     /locus_tag="PLH31_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR91065.1"
                     /translation="GLIGDLTRWVVRRALADQARLRDLGLEVEIHINLSGQMVADETF
                     ALWLLETIDRDSAGRIGLEITETAVINEPERALANLQSFAAAGLKIAIDDYGSGLSSL
                     SYLKQLPAHELKIDKLFISQLTSSHRDPLLVRSTIDLAHAL"
BASE COUNT          251 a          432 c          392 g          271 t
ORIGIN      
        1 gcgccagccg gatggctgcc cctacatgaa acgcgcaaga ttaatctctc cctcagcaca
       61 agcacttagg gtgatggaat gggcaaccag actgttgcaa tttcaaccgc cgatgcgagc
      121 gatccgggcc acgcccagga tttctttgac ctggccgaag cgctgctgaa tttggcggcc
      181 ggacagtatg gcgtccgtgc gccgccatct tcccatccca gcgccgagca cgacgccttc
      241 aatcgcctcg cagagcatct ggacaaggca tcatcagccg atctgccagc ttcgcttctg
      301 accctcaatc cccgtccctc cgacgcagct ggcctgagct atatcgccat cctcggcctt
      361 gacggcttca gtcgtcttcg ccggcaaatt ggctcccagg tcgccagtca gatcctcaag
      421 accctaggcg aacgtgtcag gctcctactt ccgggtgtaa agcttggtcg catcggtcga
      481 acaaaccttg agtttgcctt ccgggccgct gacgatcttg aagccgtgag tctcttgagc
      541 gcgctgcgcc tcgagctgga ccgccgcctc gatctggcgg acggacagag tttcgatctc
      601 gacgtcgcct ttggcgccgc gcgccatgat cgatgcggag agtttgtgat cgactgcgcg
      661 gcagaagctc tggtccaggc acagtcgcag gccacgaagg ttcagatcta tcgcgaggaa
      721 gaccgcgaac gcgcagcggc aagcttggcc ttgttgcgcg acctgcgtga agccatcgcg
      781 caggatggcc tggcattggc ctatcaaccc aagctgcggc ttagcagcga caggatggag
      841 gttgccgagg ccttggtgcg ctggaaccat cctgtccggg gcgccgtctc gcctgatgag
      901 tttatcggcc tggccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtctaatc
      961 ggagatctca cgcgatgggt cgtccgtcgc gccctcgccg atcaggcgcg cctgcgagac
     1021 ttggggcttg aggttgagat acacatcaac ctctccggcc aaatggtcgc tgacgaaacc
     1081 ttcgctttgt ggctgctgga gaccatcgac cgagatagcg ccggccgtat tgggctggag
     1141 atcaccgaaa ccgccgtcat caacgaacca gagcgcgccc tggccaacct ccagagcttc
     1201 gccgccgctg gtttgaagat cgccatagat gactacggct cgggattgtc ttcgctgtcg
     1261 tacctcaagc aactgccggc ccacgagctg aagatcgaca agctgttcat ttcgcagctg
     1321 accagcagtc atcgcgaccc gttgctggtg cgttcgacga tcgatctcgc gcatgccct
//
LOCUS       DAOMEO010001000         1379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9585_length_1379_cov_2.138973, whole genome shotgun sequence.
ACCESSION   DAOMEO010001000 DAOMEO010000000
VERSION     DAOMEO010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9585_length_1379_cov_2.138973"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>307)
                     /locus_tag="PLH31_17715"
     CDS             complement(78..>307)
                     /locus_tag="PLH31_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HQR91066.1"
                     /translation="GPKGAIWIVAQKGKGAPLKDTEILAAARGYGLVDTKVCSFSKTH
                     SALRFVKRKAGPPAASKPVEDDAAAEFDDEV"
     assembly_gap    308..317
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<318..698)
                     /locus_tag="PLH31_17720"
     CDS             complement(<318..698)
                     /locus_tag="PLH31_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HQR91067.1"
                     /translation="MGKEARGVWGHLADGDCEGKLLWEHPKLIFRGAHRGIYQGHALR
                     YIKVEGNDLVLNDGTRFEMEPGEADKWLHAILNPPGRLDKLGVKPDQTVVVLGLEDEE
                     FLDELSGRVEPVEADEDIDLLFLGV"
     gene            complement(703..>1379)
                     /locus_tag="PLH31_17725"
     CDS             complement(703..>1379)
                     /locus_tag="PLH31_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQR91068.1"
                     /translation="INDDQDIRCVILTGAGRAFSAGGDVKAMKAREGAFSGNGVKVRD
                     GYRKNIHRIVRAIYGLEVPSIAAVNGAAIGLGCDVACMTDIRIGADTAKFGVTFLKLG
                     LIPGDGGAWLMPRTIGMSRAAELLFTGDVIDAAKAAEWGLISKAVPADALMDEAMALA
                     TRIAAQPPHALRMAKSLLKHGTTASYDTLMEMSAAAQAIAHHTEDHMEGVDAILEKRA
                     PVFKGA"
BASE COUNT          191 a          484 c          449 g          245 t
ORIGIN      
        1 cacaccctgc tttacgcaat ctccaatcca cgcctcgcaa tactcgcgcc caaccttccc
       61 catcgccagg ttctccctca gacctcgtcg tcgaactctg cggcagcgtc atcctcgacc
      121 ggctttgacg ccgccggcgg ccccgccttg cgcttgacga accgcagcgc cgagtgagtc
      181 ttggagaagc tgcacacctt ggtgtcgacc aggccatagc cccgcgccgc cgccaggatc
      241 tcggtgtcct tcagcggcgc gcccttgccc ttctgggcca cgatccagat cgcgcccttg
      301 gggccaannn nnnnnnngac gcccaggaac agcaggtcga tgtcctcgtc ggcctcgacc
      361 ggttcaacgc ggcccgagag ttcatcgaga aactcttcgt cctcgaggcc cagcaccacc
      421 accgtctggt ccggcttgac gcccagcttg tccaggcggc cgggcgggtt gaggatggcg
      481 tgcagccact tgtcggcctc gcccggctcc atctcgaagc gcgtgccgtc gttgagcact
      541 aggtcgttgc cctcgacctt gatgtagcgc agggcgtggc cctgatagat gccgcgatgg
      601 gccccgcgga agatcagctt gggatgctcc cacaagagct tgccctcgca gtcaccgtcc
      661 gccagatgcc cccagacgcc gcgcgcttcc ttgcccatga cctcaggcgc ccttgaagac
      721 cggcgcgcgc ttttcgagga tcgcgtcgac gccttccatg tggtcctcgg tgtggtgggc
      781 gatggcctgg gcggccgcgc tcatctccat cagggtgtcg tagctggcgg tcgtgccgtg
      841 cttgagcagg ctcttggcca tgcgcagggc gtgcggcggc tgggccgcga tgcgggtggc
      901 cagggccatg gcctcgtcca tcagggcgtc ggcgggcacg gccttgctga tcaggcccca
      961 ttcggcggcc ttggccgcgt cgatgacgtc gccggtgaac agcagttcgg cggcgcgact
     1021 catgccgatg gtgcgcggca tcagccaggc gccgccgtcg ccggggatca ggcccagctt
     1081 caggaaggtg acgccgaact tggcggtgtc ggccccgatg cggatgtcgg tcatgcaggc
     1141 cacgtcgcag cccaggccga tggccgcgcc gttgacggcg gcgatggaag ggacttccag
     1201 gccgtagatc gcccggacga tgcggtggat gttcttgcga tagccgtcgc ggaccttgac
     1261 gccgttgccg ctgaacgcgc cctcgcgggc cttcatcgcc ttgacgtcgc cgccggccga
     1321 gaaggcccgg cccgcgcccg tcaggatcac gcagcggatg tcctggtcgt cattgatcg
//
LOCUS       DAOMEO010001001         1376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9616_length_1376_cov_1.963664, whole genome shotgun sequence.
ACCESSION   DAOMEO010001001 DAOMEO010000000
VERSION     DAOMEO010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9616_length_1376_cov_1.963664"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLH31_17730"
     CDS             <1..488
                     /locus_tag="PLH31_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQR91069.1"
                     /translation="ITSMLQGVVQRGTAASVSSLGRPLAGKTGTTNEYRSAWFVGYSP
                     NLVVGVFVGFDDNRSLGAGETGATDAVPVFMDFMREALKGQPVVDFKPPKTAKFATVR
                     GIREAFRPGTEPKVAAPAAVTGPQPYSDAWPNGVPSGAPSAAEAARPPPRAPDPLSGL
                     Y"
     gene            564..1376
                     /locus_tag="PLH31_17735"
                     /pseudo
     CDS             564..1376
                     /locus_tag="PLH31_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079283.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT          241 a          446 c          451 g          238 t
ORIGIN      
        1 agatcacctc gatgcttcag ggcgtggttc agcgcggcac ggccgcatcg gtgagttcgc
       61 ttggtcgtcc ccttgcgggc aagacgggca ccaccaacga ataccgcagc gcctggtttg
      121 ttggctattc gcccaatctt gtggtcggcg tcttcgtggg ctttgacgac aatcgctcgc
      181 ttggcgcggg cgaaacgggg gcgacggacg ctgtgccggt gttcatggat ttcatgagag
      241 aagccctcaa gggccagccc gtcgtggact tcaaaccgcc caagacagcc aagttcgcta
      301 cggtgcgggg cattcgtgaa gccttccgtc ctggcaccga gcccaaggta gcggcccccg
      361 ccgccgtgac cggtccgcaa ccctattccg acgcctggcc aaacggcgtg ccctccggcg
      421 cgcccagcgc cgcggaagcc gcgaggccgc cgccccgggc gcccgatccg ctgagtggac
      481 tctactgatc catcatcggc gttgcgccgg tagccgacgc ggggtatctc ccgcgccctt
      541 tcctattgtc acggagtaac gtgatgcgac cggatgttga ggcctctgcg gccgacatcg
      601 agcagtccgt gggactgctc aggaggcgtc tttgactggg atgccgcact tcgaaagctc
      661 gatgagctga acgctcgggt cgaagacccg acactttggg acaaacccgc cgaagctcag
      721 gccgtctcgc gcgagcgcgc cagcctgtcg gcgcgcgtcg aggccgttca ggtgctcgaa
      781 cagggcctca aggacgcgct ggaatatgcc gagctggccg acatggaggg ggatgaagcc
      841 cttctggagg acgcccgcgc ccagctgaag gacctcaagg accgagccgg tcgcgctgag
      901 cttgaggcct tgctctccgg cgaggccgac ggcaacgact gctatgtgga aatcaattcc
      961 ggcgctggcg gcaccgagtc ctgcgattgg gcgggcatgc tgctgcgcat gtattcgcgc
     1021 tgggccaatg ctcacaagat gagcgtggag ctggtcgaag agacctcggg cgaccaggtt
     1081 ggcatcaagt cggcaaccct gctgatcaag ggtcagaacg cctatggctg gctgaagacc
     1141 gaggccggcg tgcaccgcct ggtgcgcatc agcccttatg acgccgccgc caagcgtcac
     1201 accagctttg cctcggcctg ggtctatcct gttgtcgacg acacgatcga gatcgagatc
     1261 aatccgtctg acgtgcgcac cgacacctac cgggccagcg gcgctggcgg tcagcacatc
     1321 aacaagaccg actcggccgt gcgcctgacg cacattccga cgggtatcgc ggtggc
//
LOCUS       DAOMEO010001002         1375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9622_length_1375_cov_2.528788, whole genome shotgun sequence.
ACCESSION   DAOMEO010001002 DAOMEO010000000
VERSION     DAOMEO010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9622_length_1375_cov_2.528788"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLH31_17740"
     CDS             <1..428
                     /locus_tag="PLH31_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2083 domain-containing protein"
                     /protein_id="HQR91070.1"
                     /translation="NITKRHSATRLQFARFGGACPLWNVHDAFARPDKLLVQLAQMPD
                     GVRYVCIASGVVKRSGSYLQPDRRYALGLGCEVQYADDLIYAEGLDLGGPSTPIGVSC
                     RICERNDCPQRAFPPVDRRFDVLEDERRIVPFSLRPENG"
     gene            complement(432..>1375)
                     /locus_tag="PLH31_17745"
     CDS             complement(432..>1375)
                     /locus_tag="PLH31_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR91071.1"
                     /translation="LNLFRVVSAALALRRDRRVIVMEGSNFPTNNYIVQGLIESLGQG
                     HQIRFAEKDTLEDAIDDDVAAVVLTQAHYKTAHVLDMAAITAKAYAHGALAIWDLCHS
                     AGAIPVDLKGCHADFAVGCTYKYLNGGPGGPAYLFVASRHQGAAAQPLTGWWGHGAPF
                     AFDRDFRPAEGIGRMLTGTQPILSMVATEAGLDMIERAGTGAIRAKSLALGKLFQDLM
                     AARLPDAGFALASPADPADRGGHVAYDHPSGYPIMKALIARGVIGDFRAPATLRFGFA
                     PLHLRFVDVWDAVDRLAQIIETELWRDPAYAAVDAVT"
BASE COUNT          233 a          437 c          484 g          221 t
ORIGIN      
        1 gcaatatcac caagcgccat agcgccacgc gccttcagtt cgcgcggttt ggcggcgcct
       61 gcccgctctg gaacgtccat gacgccttcg cacggccaga caagctgctg gtgcaactgg
      121 cgcagatgcc ggacggcgtg cgctatgtct gcatcgccag cggggtggtg aagcgctcgg
      181 ggtcctatct gcagcccgac cgacgctacg ccctggggct cggctgcgaa gttcagtatg
      241 ctgacgacct gatctatgcc gaaggtctcg acctgggtgg tccttcaacc cccatcggcg
      301 tcagctgccg gatctgtgaa cgcaatgact gcccgcagcg cgcgttcccg ccggttgacc
      361 gacggttcga tgttctggag gacgagcgcc ggatcgtgcc gttctcgctt cgcccggaga
      421 acggataggt atcaggtcac ggcgtcgacg gcggcatagg ccgggtcgcg ccagagttcc
      481 gtctcgatga tctgggcaag gcgatcaacg gcgtcccaca catcgacgaa ccgaaggtgc
      541 aggggcgcaa agccaaagcg cagggtggcg ggagcccgga agtctccgat gacgccccga
      601 gcgatcaggg ccttcatgat cgggtagccc gaagggtgat cgtaggcgac atggccgccg
      661 cgatcagcgg gatcggccgg gctggcgagc gcgaagccgg cgtccggcag gcgcgccgcc
      721 atcaggtcct ggaacagctt gccgagagcc agggacttgg cgcggatcgc cccggtcccg
      781 gcgcgctcga tcatgtccag gccagcttcg gtggccacca tgctgaggat cggctgggtg
      841 cccgtcagca tgcgaccgat gccttcggcc ggccgaaagt cgcgatcgaa ggcgaacggc
      901 gcgccatgac cccaccagcc ggtcaggggc tgggccgccg cgccctgatg acgactggcc
      961 acgaacagat aggccggacc gccggggccg ccgttgaggt acttgtaggt gcagcccact
     1021 gcgaaatcgg cgtgacagcc cttcaggtcg accgggatcg cgccggcgct gtggcacaga
     1081 tcccagatcg caagcgcgcc gtgggcatag gccttggcgg tgatggccgc catgtcgagc
     1141 acgtgggcgg tcttgtagtg ggcctgggtc aggacgacgg cggcgacatc gtcatcgatc
     1201 gcgtcttcga gggtgtcctt ctcggcgaag cggatctggt gaccctggcc cagcgactcg
     1261 atcagaccct gaacgatgta attgttggtc ggaaagttgc tgccttccat cacgatcacg
     1321 cgccggtccc ggcgcagggc cagggcggcg gagacgacgc ggaagaggtt caggc
//
LOCUS       DAOMEO010001003         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9641_length_1374_cov_2.055345, whole genome shotgun sequence.
ACCESSION   DAOMEO010001003 DAOMEO010000000
VERSION     DAOMEO010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_1374_cov_2.055345"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLH31_17750"
     CDS             <1..965
                     /locus_tag="PLH31_17750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517389.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQR91072.1"
                     /translation="LGSVLMLAAILAMITIAGTSSIPELMAFKFAPWLQTWLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFMRFSLPMFPNASEMFQ
                     PLVFTMSAIAIVYTSLVAFRQTDIKKLIAYSSVAHMGFVTMGIFSGNAAGEQGAIFQM
                     LSHGVISGALFLCVGVVYDRMHTREIAFYGGLTNRMPWYAATFLLFTMGNVGLPGTSG
                     FVGEILTMTGVYQVSTWTALFAASGVILSAMYALTLYRRVMFGKIVNPELKTITDLDK
                     REILIFAPLIISTLVLGIYPNFVFDLTASSVDALVGAWRAAVAG"
     gene            973..>1374
                     /locus_tag="PLH31_17755"
     CDS             973..>1374
                     /locus_tag="PLH31_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQR91073.1"
                     /translation="MTLSANLSLILPEVILAVSALALLVWGAFQGRVTAAFTLASVAA
                     LIAAAVAAVMGPHGHAFNGVYSADAAATYAKVAIFFSSAVGIVLGDRWLAQRNDQKFE
                     YAVLVILAALGMGVTASAGDLISLYIGVELQS"
BASE COUNT          190 a          458 c          421 g          305 t
ORIGIN      
        1 tgcttggttc ggtgctgatg ctggcggcga tcctggcgat gatcaccatc gccggtacct
       61 cctcgatccc cgagttgatg gccttcaagt ttgcgccctg gctgcagacc tggctgtggc
      121 tggccttctt cgccagcttc gcggtcaaga tgccgatgtg gccggttcac acctggcttc
      181 ccgacgccca cgtcgaggcg ccgacagccg gttcggtcat cctggcgggt atcctgctga
      241 agatgggcgg ctacggcttc atgcgcttca gcctgccgat gttccccaat gcctcggaga
      301 tgttccagcc gctggtgttc acgatgtcgg ccatcgccat cgtctacacc tcgctggtcg
      361 ccttccgtca gaccgacatc aagaagctga tcgcctattc gtcggtggcc catatgggct
      421 tcgtgaccat gggtatcttc tcgggcaatg cggccggcga gcagggcgcc atcttccaga
      481 tgctgagcca cggcgtgatc tccggcgcgc tcttcctctg cgtcggcgtc gtctatgacc
      541 gcatgcacac ccgcgagatt gccttctatg gcggcctgac caaccgcatg ccctggtacg
      601 cggcgacctt cctgctgttc accatgggca atgtcggcct gccgggcacc tcgggcttcg
      661 tcggcgaaat cctgaccatg accggcgtct accaggtctc gacctggacg gccctgttcg
      721 cggccagcgg cgtgatcctg tcggcgatgt atgccctgac gctctatcgc cgggtcatgt
      781 tcggcaagat cgtcaatccg gagctcaaga cgatcaccga tctcgacaag cgcgagatcc
      841 tgatcttcgc cccgctgatc atctcgaccc tggtgctcgg catctatccg aatttcgtat
      901 ttgacctgac cgcgtcgtcc gtcgacgcgc tggttggcgc ctggcgcgcc gccgtggccg
      961 ggtgagggac tgatgacgct ttcagccaat ctttcgctga tcctgcctga ggtcatcctc
     1021 gcggtttcgg cccttgccct gctggtctgg ggcgcctttc aggggcgtgt caccgccgct
     1081 ttcacgctgg ccagtgtcgc ggccctgatc gcggcggctg tggcggccgt gatgggccca
     1141 cacggtcacg ccttcaacgg cgtctattcg gccgatgcgg cggcgaccta cgccaaggtc
     1201 gcaatcttct tctccagcgc ggtcggcatc gtgctgggag accgctggct ggcccagcgc
     1261 aacgaccaga agttcgagta tgccgttctg gtgatcctgg cggcgctggg catgggcgtc
     1321 accgcgtcgg ctggcgacct gatcagcctc tatatcggcg tcgaactgca atcg
//
LOCUS       DAOMEO010001004         1374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9642_length_1374_cov_2.042456, whole genome shotgun sequence.
ACCESSION   DAOMEO010001004 DAOMEO010000000
VERSION     DAOMEO010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9642_length_1374_cov_2.042456"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLH31_17760"
     CDS             <1..572
                     /locus_tag="PLH31_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQR91074.1"
                     /translation="VIVRWNPRTLFAKIFEEPAELDGFLIHVCTMEWHGETDRGLSFA
                     DNIARLTPLHPHYAGQIAAWWDRWPEMFSGPIPETEAVMDDLAARGVPQFGLTNMSSE
                     TWPDVQAMSPVFRHFQDVIVSAEEKVIKPERRIYEIVLERTGLTPGDLLFVDDNAANI
                     AAADAMGFHTHLFTDPAALRPMIQAHGLL"
     gene            635..901
                     /locus_tag="PLH31_17765"
     CDS             635..901
                     /locus_tag="PLH31_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HQR91075.1"
                     /translation="MADDAAHADVLNSTAQGQLKSIIERIERLEVEKAEISEQIKEVY
                     NEAKGNGFDVKILRKVVRILKQDRAKRQEEDAILDLYLSAVGAI"
     gene            957..1295
                     /locus_tag="PLH31_17770"
     CDS             957..1295
                     /locus_tag="PLH31_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91076.1"
                     /translation="MRAKPPDPRAQAKRAALNALKRARRAADKSGIALSDWEGEFLNS
                     VTQRVETYGRAFGDPEKGGRDQALSGNQAIKLKEIVAKAKGEKKPMKRGRGFGRRAEP
                     QSTTSTEGDE"
BASE COUNT          270 a          442 c          443 g          219 t
ORIGIN      
        1 atgtcatcgt gcgctggaat ccgcgcacgc tcttcgcgaa aatcttcgag gaacccgccg
       61 aactcgacgg gttcctcatc catgtctgca ccatggagtg gcatggcgag actgaccgag
      121 gcctgtcgtt cgccgacaac atagcccggc tgacgccgct gcatccccac tatgccgggc
      181 agatcgccgc ctggtgggat cgctggccgg agatgttctc cggcccgatt cccgagaccg
      241 aggccgtgat ggatgacctg gccgcgcggg gcgtcccgca gtttggcctc accaacatgt
      301 ccagcgagac ctggcccgac gtccaggcca tgagcccggt gttccgccac ttccaggacg
      361 tgatcgtctc ggccgaggaa aaggtcatca agcccgagcg gcggatctac gagatcgtgc
      421 tggagcggac aggactgacg ccgggcgacc tgctgttcgt cgacgacaac gccgccaata
      481 tcgccgccgc cgacgcgatg ggattccata cacatctgtt cacagatccg gcggccctgc
      541 ggccgatgat tcaggcgcac gggctgttgt gagggaaggg cggcccgact atggtcggcg
      601 acccaatttc cactccgact gagagctttc cgtcatggcc gacgacgccg cccacgccga
      661 cgttctgaac tccaccgccc agggccagct gaagagcatc atcgagcgca tcgagcgcct
      721 cgaggtcgag aaggccgaga tttccgagca gatcaaggaa gtctacaacg aggccaaagg
      781 taacggcttc gacgtcaaga tcctgcgcaa ggtggtccgc atcctcaagc aggatcgcgc
      841 caagcgccag gaagaggacg ccatcctcga cctctatctg tcggccgtcg gcgcgatcta
      901 aacggggtgt ttttgcgggg tgcgtcctgg cgcggcttcg gggataatcc ccgtcgatgc
      961 gcgccaagcc ccctgatccc agagctcaag ccaaaagggc cgcgctgaac gcgctgaaac
     1021 gcgccaggcg ggccgccgac aagagcggga tcgcgctgtc cgactgggag ggcgagttcc
     1081 tcaactcggt cacccagcgc gtcgagacct atggccgggc gtttggcgat cccgaaaagg
     1141 gcggtcgcga ccaggccctg tcgggcaacc aggcgatcaa gctcaaggaa atcgtcgcca
     1201 aggccaaggg cgagaagaag cccatgaagc gcggccgggg ctttggccgc cgcgctgagc
     1261 cgcaatcgac cacgtcgacc gagggtgacg aatagccggt atggttgtgc ggcgtgaatg
     1321 cgggcgcaag gtcgatgcaa ctctgaattg caagccgtgc gatcccccat gtcg
//
LOCUS       DAOMEO010001005         1373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9653_length_1373_cov_2.556904, whole genome shotgun sequence.
ACCESSION   DAOMEO010001005 DAOMEO010000000
VERSION     DAOMEO010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_1373_cov_2.556904"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>919
                     /locus_tag="PLH31_17775"
     CDS             <1..>919
                     /locus_tag="PLH31_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR91077.1"
                     /translation="GGVSGAMAAWCLAPVAAASTLVSPRRLAEGAALSLIGGCVAALT
                     QLSGLAPAAPTGALAFILGFLALTTTGLGLASGLLIGHRRFGARDVRHSAEMASLDVM
                     LDGLPQLAIAVTTRGQVTAVRGAPPPGVTVADIVNRGLAGAAAPNDRQKVGGAIAQAF
                     REGHVSLTFSPALGVDRIVALDLHRASPVLLVGLLRDVTVERNRETALDRARADAEAL
                     AAGRARFLANMSHELRTPLNAIMGFSDIMRSRMFGPLTDRYGEYAELIHESGGHLLDL
                     INDVLDMSKIEAERFELSRGVFDAREAMTA"
     assembly_gap    920..929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <930..>1373
                     /locus_tag="PLH31_17780"
     CDS             <930..>1373
                     /locus_tag="PLH31_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91078.1"
                     /translation="VQADSAGVQLRGVLPPNDLEVDADRRALKQIVLNLISNALKFTP
                     RGGQVTVTADGYDGVLEIVVADTGVGISPEDLERLGRPYEQAGGADQRAMGTGLGLSL
                     VRAFAKLHGGEMHIESRMGAGTSVSVRLPVLLAPLKAPPPPSEPL"
BASE COUNT          181 a          500 c          461 g          221 t
ORIGIN      
        1 gggcggggtg tcgggggcca tggccgcctg gtgcctggcg ccggtcgccg ccgcctcgac
       61 cctggtctcg ccacgccgcc tcgccgaggg cgccgccctg tcgctgatcg gcggctgcgt
      121 ggcggccctg acccagcttt cgggactggc tccggccgcg cccacaggcg ctctggcctt
      181 cattctgggc ttcctggccc tgaccacaac cgggcttggc ctggcttcgg gcctactgat
      241 tggccatcgc cggttcggcg cacgcgatgt gcgccatagc gccgagatgg cttcgctgga
      301 cgtgatgctg gacggcctgc cccaactggc catcgccgtg accacgaggg gccaggtcac
      361 cgccgtgcgc ggcgcgcccc cgccgggcgt caccgtcgcc gatatcgtca atcgcggcct
      421 ggccggcgcc gccgcgccca acgaccgcca gaaggtcggc ggagccatcg cccaggcttt
      481 ccgcgagggt catgtcagcc tgaccttcag ccccgccctt ggcgttgacc gcattgtcgc
      541 cctggacctg caccgcgcct cgcccgtctt gctggtaggc ttgctgcgcg atgtcaccgt
      601 cgagcgaaac cgtgaaaccg ctctggatcg cgcccgcgcc gacgccgagg ccctggccgc
      661 cggccgcgcc cgcttcctgg ccaatatgag ccacgaactg cgcacgccgc tgaacgccat
      721 catgggcttt tccgacatca tgcgctcgcg gatgtttggc cccctgaccg accgctacgg
      781 cgaatatgcc gagctgatcc atgagagcgg cggtcacctg ctggacctga tcaacgacgt
      841 tctggacatg tccaagatag aggccgagcg cttcgagctt tcgcgcgggg tgttcgatgc
      901 tcgcgaggcg atgacggcgn nnnnnnnnng cgttcaggcc gatagcgcgg gggtgcagtt
      961 gcgcggcgtg ctgccgccca acgacctgga ggtcgacgcc gatcgtcgcg cgctgaagca
     1021 gatcgtcctg aacctgatct ccaacgccct gaagttcacc ccgcgcggcg gccaggtcac
     1081 ggtcacggct gatggttatg acggcgttct ggagatcgtc gtcgccgaca ccggcgtcgg
     1141 catcagcccc gaagacctgg aacgcctggg ccgtccctac gaacaggccg gcggcgccga
     1201 ccagcgcgcc atgggcacgg ggctgggcct gtctctggtg cgcgccttcg ccaagctgca
     1261 cggcggcgag atgcatatcg aaagccgcat gggcgccggc accagcgtct cggtgcgcct
     1321 gccggtactg ctggccccgc tgaaggcgcc gcccccgccg tccgagccgc tgg
//
LOCUS       DAOMEO010001006         1372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9662_length_1372_cov_2.329537, whole genome shotgun sequence.
ACCESSION   DAOMEO010001006 DAOMEO010000000
VERSION     DAOMEO010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9662_length_1372_cov_2.329537"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLH31_17785"
     CDS             <1..540
                     /locus_tag="PLH31_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR91079.1"
                     /translation="AIGFIFLFTVGGVTGVVLSNAGIDYSLHDTYYVVAHFHYVLSLG
                     AVFAIFAGFFYWFEKMWGVKYNEFLGCLQFWIMFIGVNLIFFPQHFLGLQGMPRRYVD
                     YPDAFAQWNYVSTVGYMITVVGVAVFLVILIEAAIRRRKAEANPWGEGATTLEWTLSS
                     PPPFHQFSEPPVIKADDHH"
     gene            643..>1372
                     /locus_tag="PLH31_17790"
     CDS             643..>1372
                     /locus_tag="PLH31_17790"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQR91080.1"
                     /translation="MSKIAAMTPETDQPLADPATARWQDFFQLLKPRVMSLVVFTGLT
                     GLLAAREPMHPVLAAVAVLCIALGAGASGALNMWFDADIDGLMRRTRGRPVPSGKVRG
                     DDAAALGVVLSLLSVMLLGMAVNWLAAGLLAFTIVFYAVVYTMWLKRWTTQNIVIGGL
                     AGALPPAIGWAAATGSAPLNAWLMVAIIFLWTPPHFWALSLYITTDYAKAGVPMLPVI
                     KGAKHTRLQILIYSLILIPVCLSPV"
BASE COUNT          196 a          463 c          428 g          285 t
ORIGIN      
        1 gcgatcggct tcatcttcct gttcaccgtc ggcggcgtga ccggcgtcgt cctgtccaat
       61 gccggcatcg actacagcct gcacgacacc tattacgttg tcgcccactt ccactacgtg
      121 ctgtcgctgg gcgccgtgtt cgccatcttc gccggcttct tctactggtt cgagaagatg
      181 tggggcgtga agtacaacga gttcctgggc tgcctgcagt tttggatcat gttcatcggc
      241 gtcaacctga tcttcttccc gcagcacttc ctgggcctgc agggcatgcc gcgtcgctat
      301 gtcgactatc cggacgcctt cgcgcagtgg aactatgtct cgacggtggg ctatatgatc
      361 accgtggtcg gcgtggccgt gttcctggtt atcctgatcg aggccgccat ccgtcgtcgc
      421 aaggccgagg ccaatccgtg gggcgaaggc gccaccaccc tggaatggac cctgtcttcg
      481 ccgccgccgt tccaccagtt cagcgaaccg ccggtgatca aggctgacga tcaccactga
      541 tcgccaagct gagctttgcc tgaaacgcgg gggcgcggtc tggaccactt aagggtccgg
      601 gccgcgccct tcgcccatga agatcgctat aattaactcc agatgtcgaa gatcgccgcc
      661 atgaccccgg aaaccgacca gccgctggcg gaccccgcca ctgcgcgctg gcaggatttc
      721 ttccagctgc tcaaaccgcg cgtcatgtcg ctggtggtgt tcaccggcct gacgggcctg
      781 ctggccgccc gcgagcccat gcatccggtt ctggccgccg tggcggtgct gtgcatcgcg
      841 ctgggggcag gggcctcggg cgccctgaat atgtggttcg acgccgatat cgatggcctg
      901 atgcgccgca cgcgcggccg gcccgtgccc tcgggcaagg tgcggggcga cgacgcggca
      961 gccctgggcg tggtgctgtc cctgctgtcg gtgatgttgc tgggcatggc tgtcaactgg
     1021 ctggccgccg gcctgctggc cttcaccatc gtcttctatg ccgtggtcta caccatgtgg
     1081 ctcaagcgct ggacgaccca gaacatcgtc attggcggct tggccggcgc cctgccgccc
     1141 gctattggct gggccgcagc gacgggctcg gccccgctga acgcctggct gatggtggcg
     1201 atcatcttcc tgtggacgcc gccccacttc tgggcgctgt cgctgtacat caccaccgac
     1261 tacgccaagg ccggcgtgcc gatgctgccg gtgatcaagg gcgccaagca cacccgcctg
     1321 cagatcctga tctatagcct gatcctgatc ccggtctgtc tgtcgccggt gc
//
LOCUS       DAOMEO010001007         1370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9683_length_1370_cov_2.986312, whole genome shotgun sequence.
ACCESSION   DAOMEO010001007 DAOMEO010000000
VERSION     DAOMEO010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9683_length_1370_cov_2.986312"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLH31_17795"
     CDS             <1..800
                     /locus_tag="PLH31_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HQR91081.1"
                     /translation="DQMDEALATYEKWGVKGIKVDFMDRDDQAMVDYYHRLLTKTGQH
                     RLMLDLHGAYRPTGLVRTYPHYVTQEGVMGAEFNKWSSRITATHNVTLPFTRMLLGPM
                     DYTPGGFRNVTPQAFRSQFILPVVKTTRGQALAMYVVYDSPLSMVSDSPVTYAASPAG
                     LDFISATPTTWDETRVLSGQIGQSIVMARRKGRDWWVGAMTNEEGRTVKVPLAFLGAG
                     AFSADIREDGDAPTELKSAVRVVSASNVLTLKLEPSGGGAIRLTPVN"
     gene            complement(913..>1370)
                     /locus_tag="PLH31_17800"
     CDS             complement(913..>1370)
                     /locus_tag="PLH31_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQR91082.1"
                     /translation="QVTKAATNAKFNGVSIADGTTAKLTFLANADGSGFTVSAKTLTL
                     AGIGLSAASTFATAAAAKTMITTVSDALGTATNKLASLGTASTGLDTHLTFVGKLQDS
                     LEAGVGNLVDADLAKESARLQSLQTKQQLGIQALSIANSSSAAILGLFR"
BASE COUNT          242 a          443 c          461 g          224 t
ORIGIN      
        1 acgaccagat ggacgaagcc ctggccacct atgaaaagtg gggcgtgaag gggatcaagg
       61 tcgacttcat ggaccgcgat gaccaggcca tggtcgacta ctaccaccgc ctgctgacca
      121 agaccggcca gcaccgcctg atgctggacc tgcatggcgc ctatcgaccc acggggctgg
      181 tccgcaccta tccgcactat gtcacccagg agggggtgat gggcgcggag ttcaacaagt
      241 ggagcagccg gatcaccgcc acccacaatg tcaccctgcc cttcacccgc atgctgctgg
      301 ggccgatgga ctatacgccc ggcggctttc gcaacgtgac gccgcaggcg ttcaggagcc
      361 agttcatcct gccggtggtg aagacgacgc gcggccaggc cctggccatg tacgtggtgt
      421 atgacagccc gctgtcgatg gtgtcggaca gccccgtgac ctacgccgcc tcgcccgccg
      481 gcctggactt catcagcgcc acgcccacca cctgggacga gacgcgggtg ctgtcgggac
      541 agatcggcca gtccatcgtc atggcccgcc gcaagggtcg cgactggtgg gtcggcgcca
      601 tgaccaatga ggaaggccgt acggtgaagg tcccgctcgc cttccttggc gccggcgcat
      661 tcagcgccga catccgcgag gatggcgacg cgccgaccga actgaagtcg gccgttcgcg
      721 tggtttcggc cagcaatgtc ctgacgctga aactggagcc gagcggcggc ggagccatcc
      781 gcctgacccc ggtaaactag aaggccaggc cctcccgaat gcaaaaaagc ggggcggaca
      841 cgaagtccgc cccgcagatg cagctgttca tgcgggcagg cccttacgcg gcctgcccgt
      901 ccagctgtcg tcctatcgga aaagacccag gatcgccgcg ctcgaggaat tggcgatcga
      961 cagggcctga atgcccagct gctgcttggt ctgcagcgat tgcaggcggg cgctttcctt
     1021 ggccaggtcg gcgtcaacca ggttgccaac accggcttcc aggctgtctt gcagcttgcc
     1081 aacgaaggtc aggtgcgtgt ccaggccggt cgaggcggta cccagcgaag ccagcttgtt
     1141 ggtcgccgtg cccagcgcgt ccgagaccgt ggtgatcatg gtcttggccg cggcggccgt
     1201 ggcgaaggtc gaagcggccg agaggccgat gccggccagg gtcagggtct tggcggagac
     1261 cgtgaagccc gaaccgtccg cgttggcgag gaaggtcagc ttggcggtcg tgccgtcggc
     1321 gatgctgacg ccattgaact tggcgttcgt ggcggccttg gtcacctgat
//
LOCUS       DAOMEO010001008         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9689_length_1369_cov_2.788432, whole genome shotgun sequence.
ACCESSION   DAOMEO010001008 DAOMEO010000000
VERSION     DAOMEO010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9689_length_1369_cov_2.788432"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLH31_17805"
     CDS             <1..583
                     /locus_tag="PLH31_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQR91083.1"
                     /translation="PSYRFYPGDEVEVTVASAPELNKSAVVQPDGRIVLSLLGPVMAA
                     DRTIFELEDAITEAYGSQLLRPQVQVSVKGTAPLKVFVGGEVGTPGVYDMPGDGDALR
                     AVIQAGGFKTSSKRSQVVIIRRGPDGRAMLRTADLLKGMTNPGGVDLIPLRRFDIVYV
                     PRNGAAETGLFMQQYFRELLPLNFSYAINGTGG"
     gene            complement(629..904)
                     /locus_tag="PLH31_17810"
     CDS             complement(629..904)
                     /locus_tag="PLH31_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HQR91084.1"
                     /translation="MMMSIEPPAAHVLPLPTPESSGDELFRMGLLYSTGQGGAPVDYV
                     SAHMLFNLAAMRGSVEAKVYRKELSQEMASEDVAEAQRAAREWLAHG"
     gene            complement(1078..>1369)
                     /locus_tag="PLH31_17815"
     CDS             complement(1078..>1369)
                     /locus_tag="PLH31_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQR91085.1"
                     /translation="NRLYGVLDKQLAGQDFICGQLSIADFACWPWVVPYKNQGIILED
                     FPNLKAWFERVGARENVQKGFKLGAELRSAGLQASGKAAEEARKMLFGQRAR"
BASE COUNT          257 a          428 c          418 g          266 t
ORIGIN      
        1 gccctcctat cgcttctatc ccggcgatga ggtcgaagtg accgtggcgt cagcgccgga
       61 attgaacaaa tccgccgtcg tccagccgga cggacgaatc gtcctgtccc tgctgggacc
      121 ggtgatggcc gctgatcgca cgatcttcga actggaggac gcaatcaccg aggcctatgg
      181 cagccagctg ctgcggcccc aggtgcaggt gtcggtgaag ggcaccgccc cgttgaaggt
      241 gttcgtgggc ggcgaagtcg gtacgccggg cgtgtacgac atgccgggcg atggggacgc
      301 gctacgcgcc gtcattcaag ctggcggctt caagacttcg tccaagcgca gccaggttgt
      361 gatcattcgt cgcggccccg acggtcgcgc catgctgcgc acggcggatc ttctgaaggg
      421 gatgaccaat cccggcggcg tggacctgat cccgcttcgc cggttcgaca tcgtctatgt
      481 gccgcgcaat ggcgcggccg aaacgggcct cttcatgcag cagtacttcc gcgaacttct
      541 gccgcttaac ttcagctacg cgatcaacgg cacaggcggc taaccttcgg cgtcgacagg
      601 cgaagccgct gaatccgggg aaccggattc agccgtgggc cagccattcg cgcgcggcgc
      661 gctgggcttc agcgacgtct tcgctggcca tctcttgcga cagctccttg cgatagacct
      721 tggcctcgac cgaaccgcgc atggcagcca ggttgaacag catgtgcgcc gagacgtagt
      781 cgacgggggc gccgccctgg ccggtcgaat acagcaggcc catgcgaaac agttcgtcgc
      841 ccgaggactc gggggtcggc agcggcagca cgtgggcggc cgggggttcg atgctcatca
      901 tcatggcgtc ttctcctctt ccaggcccct ctgacgttct gtcggatcac aggcctgaaa
      961 gcagaaaagt cccccgccgc tgaatatata gttaagcccc ggaacatcat gaattcaaaa
     1021 acgaatacat ggattcttca ggggtgttac tcggcgtaaa cgcctatata cagcgagtta
     1081 acgcgctcgc tgcccgaaga gcatctttcg agcctcttcc gccgcttttc cggaggcctg
     1141 aagccctgcg gaacgcagct cagcccccag tttgaagcct ttttgcacgt tttcgcgggc
     1201 tccgacccgc tcgaaccaag ctttcaggtt cgggaagtct tccagaatga tgccctggtt
     1261 tttgtaaggc acaacccatg gccagcaggc gaagtcggca atggacaatt ggccgcagat
     1321 gaaatcctgc ccggccagtt gcttgtcgag cacaccatag agccggttt
//
LOCUS       DAOMEO010001009         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9690_length_1369_cov_1.935312, whole genome shotgun sequence.
ACCESSION   DAOMEO010001009 DAOMEO010000000
VERSION     DAOMEO010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9690_length_1369_cov_1.935312"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLH31_17820"
     CDS             complement(<1..468)
                     /locus_tag="PLH31_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopD"
                     /protein_id="HQR91086.1"
                     /translation="MIESLVVLARIVQYAGTAMLFGAPLFYLYGLPADGPGAARTNAG
                     TKRLVVVAGLLAAIGCGLALLAQTAMMTGALADALDPEALVMVATGTQFGMSVMIRAT
                     ASLAALALAATLHPSTNLWRASAGLGAVALASFAWSGHGADGEGAAGLFKLAAD"
     gene            complement(473..841)
                     /gene="copC"
                     /locus_tag="PLH31_17825"
     CDS             complement(473..841)
                     /gene="copC"
                     /locus_tag="PLH31_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HQR91087.1"
                     /translation="MTTKTTSTFLAMIAAGGLMLAASQAAAHAKLVSANPAPDATVAA
                     PKAISLKFSEKLEANFSSFEIAKASGGAVPVKIKVAKGGMVIDGALAAPLAPGAYKVS
                     WRVVTADAHRINGAYSFTVR"
     gene            complement(944..>1369)
                     /locus_tag="PLH31_17830"
     CDS             complement(944..>1369)
                     /locus_tag="PLH31_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HQR91088.1"
                     /translation="AGATTLLAGCAQGAEPLAIQVYKTPTCGCCTKWVDALRAAGLKP
                     SINELDDLTPVRATYGVADTLSSCHTARISGYTIEGHVPPSDILRLLQERPKALGLAV
                     PGMPIGSPGMEMPGAAAEPYATLLLLDAKGATRVFARHG"
BASE COUNT          224 a          451 c          486 g          208 t
ORIGIN      
        1 gtcggcggcc agcttgaaga ggcccgccgc cccttcgccg tcagcgccgt gtccgctcca
       61 ggcgaagctg gccagggcta cagcacccag cccggcgctg gcgcgccaga gattggtcga
      121 gggatgcaag gtcgcggcga gcgccaaggc cgccaggcta gcggtcgcgc ggatcatgac
      181 cgacatgccg aactgggtgc cggtcgccac catgactaga gcctctgggt cgagtgcgtc
      241 ggcaagcgcc ccggtcatca tcgccgtctg ggccagcagc gccaagccgc agccgatcgc
      301 cgccagcagg ccggcgacca cgaccagtcg cttcgttccg gcgttggttc gcgccgcgcc
      361 ggggccatct gcgggcagac cgtagaggta gaagagcggc gcgccaaaca acatcgccgt
      421 cccggcatac tggacaatgc gcgccagaac gacgagcgac tcgatcatag tcctagcgga
      481 ccgtgaagct gtaggcgccg ttgatgcgat gggcgtcggc ggtgaccacg cgccagctga
      541 ccttataggc acccggcgcc agcggcgcgg ccagggcgcc gtcgatgacc atcccgccct
      601 tggcgacctt gatcttcacc ggcacggcgc cgcccgaggc tttggcgatt tcgaagctgg
      661 agaaattggc ctcgagtttc tcgctgaatt tcaggctgat cgccttaggg gcggcgaccg
      721 tggcgtcggg cgcgggattg gcgctgacca gcttggcgtg ggcggcggcc tgggaagcgg
      781 cgagcatcag gccgcctgcg gcgatcatcg ccaggaaggt cgaagtggtc ttggtagtca
      841 tccgatgtct ccaaggtgcg acgccccggt cgcccacaga tcatacgcac ggcccaggcc
      901 gtgtccctcg cggcgagacg caaaaatcga tgtcgggcgc cgatcagcca tggcgggcga
      961 aaacccgggt cgcgcccttg gcgtcaagca gcagcagcgt cgcgtagggc tcggcggcgg
     1021 ccccaggcat ttccatgccg ggcgatccga tcggcattcc agggaccgcc aggcccaacg
     1081 ccttcgggcg ttcttgcaac agccgaagaa tgtcggacgg cggtacgtgg ccctcgatgg
     1141 tgtagccgct gatccgtgcg gtgtggcacg aggacagggt gtcggcgaca ccgtaggtcg
     1201 cgcgcaccgg cgtcagatca tcgagctcgt tgatcgacgg cttgaggccg gctgccctca
     1261 gggcgtcgac ccacttggtg cagcagccgc aggtcggggt cttgtaaacc tggatagcca
     1321 ggggttcggc gccttgcgcg cagcccgcca gcagagtggt ggcgccggc
//
LOCUS       DAOMEO010001010         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9732_length_1365_cov_2.611450, whole genome shotgun sequence.
ACCESSION   DAOMEO010001010 DAOMEO010000000
VERSION     DAOMEO010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9732_length_1365_cov_2.611450"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..751
                     /locus_tag="PLH31_17835"
     CDS             77..751
                     /locus_tag="PLH31_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQR91089.1"
                     /translation="MIPLDDRGLLLGDGLFETMLALDGAIPLVEAHLDRLAAGCGALG
                     LPALDREAARALCLSAPPPAGRAAVRLTVTAGSGGRGLDRPAQIAPRLRATAALSTPI
                     ETPASLIIARTRRNEGSPASRLKTLAYLDNVLARAEATAAGADEAVMLNNAGRVACAA
                     GGRRRDLPDQQPDRRAAGLAAGGAHPCAASADGAAERRLFLTRLAGRGDPQLTRITVS
                     LLRGRR"
     gene            complement(706..1275)
                     /locus_tag="PLH31_17840"
     CDS             complement(706..1275)
                     /locus_tag="PLH31_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HQR91090.1"
                     /translation="MPHKLTPRPRKGLKGFWRDRRGVSAVEFALIAPVLVIFYCGLAE
                     LTQAMMAQRRLSNIASSIGDLVAQSAQLNDARRDDIFTIGNTIMAPFPTTTLRMCLVS
                     ISSDATGKDKVDWSENRNSHGNCPAKGTVLGSTVIPVGVLPANQSVIMAKASYGYTSP
                     IKFILPSTLNFYRTFYLRPRKSETVMRVS"
BASE COUNT          192 a          454 c          496 g          223 t
ORIGIN      
        1 gcgacccgca tgccgaacgc ctggagaccg aaaccaagat cgccgccctg aagcgcgcgc
       61 tgaccgaggc tgtcagatga tccccctcga cgatcgcggt ctgttgctgg gcgacgggct
      121 gtttgagacc atgcttgcgc tcgacggcgc catcccgctg gtcgaggccc acctggaccg
      181 cctggccgcc ggctgcggcg ctctgggttt gccagctctg gatcgcgagg cggcgcgggc
      241 gttgtgcctt tccgcgccgc cgcccgcggg gcgggccgcc gtgcgtctga ccgtgacggc
      301 ggggtcaggc gggcgggggc tggatcgtcc ggcccagatc gcgccccgcc tgcgggcgac
      361 cgccgccctt tcgacgccca tcgagacccc ggcgagcctg atcatcgccc ggacccggcg
      421 caacgagggc tcgccggcct cgcgcctgaa gaccctggcc tatctcgaca atgtgctggc
      481 ccgcgccgag gcgacggcgg cgggagctga cgaggcggtg atgctgaaca atgccgggcg
      541 cgtcgcctgc gcggctggcg ggcgccgacg cgatcttcct gaccaacagc ctgatcggcg
      601 tgcggccggt ctcgcggctg ggggcgcgca tccttgcgcc gcatccgctg acggagcggc
      661 tgagaggcgt cttttcctca cccgcctagc ggggagaggc gaccctcagc tcacccgcat
      721 cacggtctcg ctcttgcgcg gccgcagata gaaggtgcgg tagaagttca gggtcgaggg
      781 caggatgaac ttgatcggcg aggtatagcc gtagctggcc ttggccatga tcacgctctg
      841 gttggcgggc agcacgccca ccgggatcac ggtggagccc agcaccgtgc ccttggcggg
      901 gcagttgccg tggctgttcc tgttctccga ccagtcgacc ttgtccttgc ccgtcgcatc
      961 cgacgagatg ctgaccaggc acatgcgcag ggtcgtggtc gggaaaggcg ccatgatcgt
     1021 gttgccgatc gtgaagatgt cgtcgcggcg ggcgtcattg agctgggcgc tctgggccac
     1081 caggtcgccg atcgatgagg cgatgttgct caggcgtcgc tgggccatca tcgcctgggt
     1141 cagctcggcc agaccacagt agaagatcac cagcaccggg gcgatcaggg cgaactcaac
     1201 ggccgagacg ccgcgacggt cgcgccagaa gcctttcagg cccttccggg ggcgaggggt
     1261 cagtttgtgg ggcatgtcac cgccgccgcg ggagtgtcgg aatagggctc gttggcgaag
     1321 gccgtcgccg ccgtcagcat gcgcttgccg ttcgaggtct gcagg
//
LOCUS       DAOMEO010001011         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9733_length_1365_cov_2.426718, whole genome shotgun sequence.
ACCESSION   DAOMEO010001011 DAOMEO010000000
VERSION     DAOMEO010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9733_length_1365_cov_2.426718"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1315
                     /locus_tag="PLH31_17845"
     CDS             89..1315
                     /locus_tag="PLH31_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91091.1"
                     /translation="MAKVLRPALIVLVLALAHAPLAAARGLPLRDQVSGVATLGVSVG
                     DNERSWVDRGLGKAGSGETVTAGAIIAWRPALGGRIGGLVTADLQSALSPSIGLDEAY
                     FTLRPSPGAAVRLSGRAGLFFPPVSLEHDGSEWSLSRSITPSAINSWVAEEVKTAGLE
                     LTARTTVTGRPMGLTLAAFQGNDTSGTLLAFRGWALHDLRATWNDTLPLPPVPTMFAG
                     KQATRTRPVDEVDGRWGVYGRLDVKPIDTLDLSLLAYDNNGDRTTVVDGQYAWRTRFL
                     QAGARWSPAARTEVLFQIMQGETSMGPRIQGLTVADVGFQSAFILMSRDIGAGAITTR
                     LEQFSIDDRSFKDSDNNAEHGWAATLAWTRPLRQTLDLVIEGVFVTSDRPDRARLGED
                     RRQDNLRLTTALRASF"
BASE COUNT          200 a          467 c          462 g          236 t
ORIGIN      
        1 gtggcggtgt tcgcctatgg ggcctggaag cggacgcgag cggtggcgca ttaaccgaaa
       61 tgaagccgcc tccggatagg ttggcggcat ggccaaggtc cttcgcccgg ctctcattgt
      121 cctggtcctc gccctggctc acgccccttt ggcggcagcc cggggcctgc ccctgcgcga
      181 tcaggtctca ggcgtagcca ccctgggcgt gtcggtcggc gataacgagc gatcctgggt
      241 cgatcgcggg ctgggcaagg ccggcagcgg ggagacggtg acggccggcg ccatcatcgc
      301 ctggcggccc gccctcggcg gccggatcgg cgggctggtc acggccgatc tccagtcggc
      361 cctgtcaccc agcatcggcc tggatgaggc ttatttcacc cttcgcccct cgccgggggc
      421 cgccgtgcga ctgtccgggc gtgcgggcct gttctttccg ccggtctcgc tggaacatga
      481 tggctcggaa tggtcgctgt cgcgctcgat cacgccgtcg gcgatcaata gctgggtggc
      541 tgaagaggtg aagaccgccg ggcttgagct gaccgcgcgc acaacggtga cagggcgccc
      601 catgggcctg accctggccg cctttcaggg caatgacacc tccggcacgc tgctggcgtt
      661 tcggggttgg gccttgcatg acctgcgcgc cacctggaat gacaccctgc ccctgccgcc
      721 cgtgccgacg atgtttgccg gcaagcaggc aacccggacc cgtccggtcg atgaggtgga
      781 tggccgctgg ggcgtctatg gccggttaga cgtcaagccg atcgacaccc tcgatctcag
      841 cctgctggcc tatgacaaca atggcgatcg cacgaccgtg gtcgacggcc agtatgcctg
      901 gcgcacacgc ttcctgcagg ctggcgcgcg ctggtcgccc gccgcccgga ccgaggtgct
      961 cttccagatc atgcagggcg agaccagcat gggaccgcgc attcagggcc tgactgtcgc
     1021 cgacgtcggc ttccagagcg cattcatcct gatgtcgcgc gacattggcg ccggcgccat
     1081 caccacgcgg ctcgagcagt tctccatcga cgaccgcagt ttcaaggaca gcgacaacaa
     1141 tgccgagcac gggtgggccg cgaccctggc ctggacccgc cccctgcgcc agactctgga
     1201 cctggtcatc gagggcgtgt ttgtcacgtc cgatcgcccg gaccgggccc ggctgggtga
     1261 ggatcgccgg caggacaacc tgcgcctgac caccgccctg cgggccagtt tctagagccc
     1321 tttccgacct gatggcttca tcagggtcgg gcgaaaaggg ctcta
//
LOCUS       DAOMEO010001012         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9765_length_1362_cov_1.952563, whole genome shotgun sequence.
ACCESSION   DAOMEO010001012 DAOMEO010000000
VERSION     DAOMEO010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9765_length_1362_cov_1.952563"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLH31_17850"
     CDS             <1..449
                     /locus_tag="PLH31_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HQR91092.1"
                     /translation="VIPHIDTFMEDGFTKEEWKMMAETKKILDPSIKLVATCVRVPVM
                     VGHAEAVNIEFETPLDEDDVREILRDADGVEVIDKREDGGYMTPKESAGEFPVYVSRI
                     RTDPTVENGIVLWCVADNLRKGAALNAVQIAQLLHDKGLIKQKLPA"
     gene            544..>1362
                     /gene="rarD"
                     /locus_tag="PLH31_17855"
     CDS             544..>1362
                     /gene="rarD"
                     /locus_tag="PLH31_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HQR91093.1"
                     /translation="MSADLSKAPNEARTALIAAVGCYVIWGVLPLYFHLLAVLGVGSW
                     EMIAHRTLWAAPWALLIVMLSRQGGQVMAVLRQPKPLLILACSTAAIFINWSIYVFAV
                     NSGHVIETSLGYYINPLMNMAAGAILFRERIRREGAIAIGLAAIGVAIQTAALGHLPI
                     ISLGVAISFCAYAILRKQVKADAQTGLFIECAYLAIPGLIYVLHLQSTGAGHFGGGAG
                     VTGLLLAAGPATVIPLALFAWSARRMPLSAMGFLQFIAPTLQFGVGVALGEAFTP"
BASE COUNT          217 a          439 c          460 g          246 t
ORIGIN      
        1 acgtgatccc ccacatcgac accttcatgg aggacggctt cacgaaggaa gagtggaaga
       61 tgatggcgga gaccaagaag atcctggatc cgtcgatcaa gctggtcgcc acctgcgtgc
      121 gcgtgccggt catggtgggc cacgccgagg cggtgaacat cgagttcgag accccgctgg
      181 acgaggacga tgtccgcgag atcctgcgtg acgccgacgg cgtggaagtc atcgataagc
      241 gcgaggacgg cggctacatg acgcccaagg aaagcgccgg cgaattcccg gtctatgtct
      301 cgcgcatccg cactgacccc accgtcgaga acggcatcgt gctgtggtgc gtggccgaca
      361 acctgcgcaa gggcgcggcg ctgaacgccg tgcagatcgc gcagcttctg catgacaagg
      421 gtctgatcaa gcagaaactc ccggcctaaa ccctcctccg caacaagcgg gtggacgatt
      481 ttggggctcc cgggacatca ggtcccggga gccctttttc attctggact gaaggacgac
      541 gccatgtcgg cggatttgag caaggccccg aacgaggccc ggacggcgtt gatcgccgcg
      601 gtgggctgct atgtgatctg gggtgtcctg ccgctgtatt tccacctgct ggcggtgctg
      661 ggcgtgggct cgtgggagat gatcgcccac cgcaccctgt gggccgcgcc ctgggccctg
      721 ctgatcgtca tgctgtcgcg gcagggcgga caggtgatgg cggtgctgcg ccagcccaag
      781 cccctgctga tcctggcctg ctcgacggcg gcgatcttca tcaactggtc gatctatgtg
      841 ttcgcggtca attccggcca cgtgatcgag accagcctgg gctattacat caacccgctg
      901 atgaacatgg cggcgggcgc catcctgttt cgcgagcgga tccgccgcga gggggccatc
      961 gccatcggcc tggccgcaat cggggtggcg atccagaccg ccgccctggg ccatctgccg
     1021 atcatctcgc tgggcgtggc catcagcttc tgcgcctacg ccatcctgcg caagcaggtg
     1081 aaggccgacg cccagacggg cctgttcatc gagtgcgcct atctggcgat cccgggcctg
     1141 atctatgtgc tgcacctgca gtcgacgggc gcgggacact tcggcggcgg agcgggcgtc
     1201 accggcctgt tgctggcggc cggcccggcc acggtgatcc ccctggccct gtttgcctgg
     1261 tcggcccggc ggatgccgct gagcgccatg ggctttttgc agttcatcgc cccgaccctg
     1321 cagttcggcg tcggcgtggc tctgggcgag gccttcacgc cg
//
LOCUS       DAOMEO010001013         1361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9771_length_1361_cov_2.297856, whole genome shotgun sequence.
ACCESSION   DAOMEO010001013 DAOMEO010000000
VERSION     DAOMEO010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_1361_cov_2.297856"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLH31_17860"
     CDS             complement(<1..185)
                     /locus_tag="PLH31_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HQR91094.1"
                     /translation="MSLQKRIWDLIVLGIFVFAAAFASAPWFAFRALKSAAQYEDVQA
                     IGELVDFRKTDTHSRVHV"
     gene            complement(286..465)
                     /locus_tag="PLH31_17865"
     CDS             complement(286..465)
                     /locus_tag="PLH31_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91095.1"
                     /translation="MPRSKTIARNRTFPAVDPVWPAARKAQLRRTVFKKMAKLRLSKA
                     PHAPDMAGEAAEAAP"
BASE COUNT          203 a          460 c          484 g          214 t
ORIGIN      
        1 acatgcaccc gagaatgagt gtcggtcttt cggaaatcga ccagctcgcc gatggcctgc
       61 acgtcctcgt actgggccgc cgacttcagg gcccggaacg caaaccaggg cgccgacgca
      121 aaggccgccg cgaacacgaa gatgcccagg acgatcaggt cccagatccg cttttgcaaa
      181 ctcatggagc gccccgattg gttaacgcgg catagaaggg ctattcgcgt cggcctcgca
      241 aggcccttcg cgcgcggcgg gaccgggacg tgggtcgcgg ccggttcagg gcgcggcctc
      301 ggctgcctcg cccgccatgt ccggagcgtg cggcgccttg ctcaggcgca gcttggccat
      361 cttcttgaac accgtgcgcc gcagttgcgc cttgcgcgcc gcaggccata cgggatcgac
      421 ggcggggaag gtgcggttgc gtgcgatggt cttgctgcgg ggcatggtga gactcctcgg
      481 ggtcgagggg caaggtctca gcctccggct ttggctggga agtttggggg ggggtgcgaa
      541 gggccggggc ccgcccggag aaggaaaaaa taccgtttcg acgcagacag acgctccgcg
      601 cgatcgttat ccggcggctt acggccctgg gcgctgttga cgccaggccg tctaggccgc
      661 cgacggcggg ccaggccagc aagcctgatc ccggaccgcg aacgtttccg tccgcctgcc
      721 gcctctcgcc gaccctgtca tcccacgtcg tttctgtgtt ccagggccga ccgccgccag
      781 aaggatccag gtgcgccggc gctcatgccg agccccggcg ccgtcgacga gcgctctccg
      841 ctcgaccacc ccccagggcc gcaaaggtcg ctggccgcgc gccctcccca tcgccctgag
      901 gtgaagacag tatagggcgg cggcgatcgc gggggagctg gggcaccttg ggcgagggat
      961 ttcagcgggt taggcgcggg aagcggggtg gataggcttc cttctcccct tgcgggagaa
     1021 ggtggcccgc gccagcgggt cggatgaggg gtcgaaagcc ctgtccgccg cgaccggcgg
     1081 atcgaccgga tgcggcgccg cctgtgcgac ccctcatccg gcgcctcgcg ccaccccgga
     1141 tcaagtccgg ggcaggctct tctcccgcaa ggggagaagg ggttttgctc tgatctcggg
     1201 ccagggtgtc cttctcccct tgcgggagaa ggtggcagcc gcaggctgac ggatgagggg
     1261 tcgaaagccc tttccgccgc gaccgactga tcgaccgggt acggcgccgc ccgcgcgacc
     1321 cctcatccgg cgcttcgcgc caccttctcc cgcaagggga g
//
LOCUS       DAOMEO010001014         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9779_length_1360_cov_2.637548, whole genome shotgun sequence.
ACCESSION   DAOMEO010001014 DAOMEO010000000
VERSION     DAOMEO010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9779_length_1360_cov_2.637548"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1122)
                     /locus_tag="PLH31_17870"
     CDS             complement(187..1122)
                     /locus_tag="PLH31_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR91096.1"
                     /translation="MFETTERSWRYDHENQDQLVTLEQLRIFLAVAERQHVTRAAEAL
                     NLTQSAVSSAITTLEGRHGVALFDRVGRSIVLNRAGEVFLAEARKVLAQAQAAEAALA
                     DLSGLERGRLSIHASQTIASYWLPPRLTLFHAAHPGIEIDVAIGNTAQVARAVAEGDA
                     EIGLVEGEVDDPVLSRSVIDEEQLSLVVAPLHPWASAHTSADHDLTKTAWVLREPGSG
                     TRAAFEHMLAARHLSLSDLKIAMVLPGNEAVRSAVEAGAGAGVMSQTVARLGVARKAL
                     VEIAFDLPPRQFYLLRHKQRYRSRAGDAFLEMAKP"
BASE COUNT          223 a          421 c          461 g          255 t
ORIGIN      
        1 ggcccgagtg gtgtcgcgga acacggggta gggggctaag gcggtcaagg tggtcattag
       61 ggtcactccg ttagagtgga ggccgattgg cggcgcgctc cttgaccgac accctgggct
      121 gtcagaccat tcttatccaa cgagacgttt gtgtatgttc gttcgatagt tccgaacgat
      181 cttgccctac ggcttcgcca tctccaggaa ggcgtctccc gcgcggctgc gatagcgctg
      241 cttgtgccgc aataggtaga actgccgagg cggcagatcg aacgcgatct caaccagggc
      301 tttgcgcgcg acgcccagtc gcgcgacggt ctgagacatc actccggcgc ccgcccccgc
      361 ttcgacggcc gagcggacag cctcattgcc cggcaggacc atggcgatct tcaggtcgga
      421 tagagacagg tggcgagcgg ccagcatgtg ctcgaacgcc gcacgggtgc cggagccggg
      481 ttcccgtaaa acccaagccg ttttggtcag gtcatgatcg gcgctcgtgt gggcggaagc
      541 ccagggatgc aatggggcga cgacaaggct cagttgctct tcatcgatca ctgaacggct
      601 cagcaccgga tcgtccacct cgccttcgac caagccaatc tcagcgtccc cctccgcgac
      661 cgcgcgggcg acctgggcgg tgttgccgat cgccacgtcg atctcgatgc cgggatgtgc
      721 ggcgtggaag agcgttaagc gcggcggcag ccagtagctg gcgatggtct ggctggcgtg
      781 gatagacagg cgtccgcgtt ccagcccact gaggtctgcc agggccgcct cggcggcctg
      841 cgcctgggcc aggaccttgc gggcctccgc caggaacacc tcaccggcgc gattgagcac
      901 gatgctgcgg ccgacccggt cgaacagggc gacgccgtgg cgcccctcga gggtggtgat
      961 ggcgctgctg accgccgact gggtcaggtt caaggcttcg gccgcccggg tgacatgctg
     1021 gcgctctgcg acggcgagga agatgcggag ctgttcgagg gtcacgagct gatcctggtt
     1081 ttcatgatca taacgccagg atcgttcggt cgtttcgaac attcttagtc gacgatcccg
     1141 ttagaccgca tgaattggcg cgtcgaaggt gcgatcgcgt ttttgaggaa ccacccatgc
     1201 aaccgcctgc gctgcctagt cgccgctcac ctcgtgtctg gatgggtctt gcggccttcg
     1261 tgtcggtcgc gggcgcttcc agcgctatcg cggcggctga cgggttcgat gcccggatgg
     1321 ccgaggccat gacgcgaatg catcacgcga tgatgctgcc
//
LOCUS       DAOMEO010001015         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9780_length_1360_cov_2.619157, whole genome shotgun sequence.
ACCESSION   DAOMEO010001015 DAOMEO010000000
VERSION     DAOMEO010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9780_length_1360_cov_2.619157"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..>1360
                     /locus_tag="PLH31_17875"
     CDS             187..>1360
                     /locus_tag="PLH31_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR91097.1"
                     /translation="MPDQLRNADTPTHAPLQARIAGVALITTVVALVAACACFMVQQW
                     SVARQESRANHQVLAQMVAAGAAAPLAAGDVIGVRNALEAVAKAPNVAGVQISDLRGR
                     VVAQVGTAPRSAADKSIPTLDRKIQLEGRQIGTLALVAREPRLEQILARDLALTGTLF
                     FGSAGLAILLANGLARRMTEPMQRLTEAMQAVAADGRFRPIEQTSKDDIFHSLTESFN
                     QLVGRLEINDQDLRGAMAELVKARDDANAANVLKSHFLANMSHEIRTPLNGVLAMAEV
                     MAMDDLKPAQRERLSVIRDSGGLLLAVLNDVLDLSKIEAGRLDLAAEDFDLATLAVSL
                     RDSFASEARDKGLAFEVAVAAEAQGVWHGDVDRLRQILGNLISNALKFTLNGAVAVR"
BASE COUNT          244 a          461 c          430 g          225 t
ORIGIN      
        1 gcaacaaaaa ccttttccac gcccatctcc cgttaagaac ctgtcaatga aggtaaacga
       61 agtcttaacg ggtgtacaac cagaaacacc tcttagcgtt gatttgtcgc gtcacccccc
      121 gggcatggaa gcgaagcgtt agggagatgg ctgcaaagtg aactcctgat ttcacttgga
      181 cggcccatgc ctgaccagct ccgcaacgct gatacgccaa cgcatgcgcc tctccaggcg
      241 cgaattgcgg gtgttgcgct gatcacgaca gtggtcgccc tggtcgccgc ctgcgcctgt
      301 ttcatggttc agcaatggag cgttgcccgg caggaatcgc gggccaatca ccaggtgctg
      361 gcccagatgg tcgcggcggg cgccgccgcg ccgctggccg ccggagacgt gatcggcgtc
      421 cgcaacgccc tggaggccgt ggccaaggcc ccaaacgtgg cgggcgtaca gatctccgac
      481 ctccgggggc gcgttgtcgc ccaggtcgga accgccccac ggtcggctgc ggacaagtcg
      541 atcccgaccc ttgatcgcaa gatccagctt gagggccggc agatcggaac tctggctctg
      601 gtggcccgcg agccgcggct ggagcagatc ctggcccgcg acctggccct gaccggcacg
      661 ctgttcttcg gctcggccgg cctggcgatc ctgctggcca acggcctggc gcgccgcatg
      721 accgagccca tgcagcgcct gaccgaggcc atgcaggctg tcgccgctga cggccgcttc
      781 cggcccatcg agcagaccag caaggacgac atcttccaca gcctgaccga aagcttcaac
      841 cagttggtcg ggcgactcga gatcaacgat caggacctgc gcggggcgat ggccgaactg
      901 gtcaaggccc gtgacgacgc caacgccgcc aacgtcctga agtcccactt cctggctaat
      961 atgagccacg agatccggac accgcttaac ggcgtgctgg ccatggccga agtcatggcg
     1021 atggatgacc tcaagcccgc ccagcgcgaa cgcctgtcgg tgatccgcga ctcgggcggt
     1081 ctgctgctgg ccgtgctgaa cgacgtgctc gacctgtcca agatcgaggc cggtcgactg
     1141 gatctcgctg ccgaggactt cgacctggcc accctggcgg tctccctgcg cgacagcttc
     1201 gccagcgaag cgcgcgacaa ggggctggcc ttcgaggttg cggtggccgc cgaggcccag
     1261 ggcgtctggc atggcgatgt cgatcgcctg cgccagatcc tgggcaacct gatctccaac
     1321 gccctgaaat tcaccctgaa cggcgccgtg gccgtacggt
//
LOCUS       DAOMEO010001016         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9781_length_1360_cov_2.511111, whole genome shotgun sequence.
ACCESSION   DAOMEO010001016 DAOMEO010000000
VERSION     DAOMEO010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9781_length_1360_cov_2.511111"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLH31_17880"
     CDS             <1..1312
                     /locus_tag="PLH31_17880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HQR91098.1"
                     /translation="VRARVADQKTRQPELYGGIDFSATPERFTDDPANSVAPASVAGA
                     APTPQHIALVRAYTLLGDRVADAYAGLMPQHGFRGLIAMLTKACDEGLEAVPDAPPEL
                     IAFIRDMEHVPDWLDMDLVRQGARLDRNAMANLSPFAIRGAFVATFMNKYAALPMALT
                     GTLGAETAARRVNETASFFTTTLLPGALERHGPGFKAAAMVRLMHSMVRFNALRRPGA
                     WDAKVYGVPIPQVDQMPAGLIPIFLLSYKLLRQGRTTFTPAERARVELARYRCYLLGL
                     PEDLLADTPVGIVQTMSARNQTLRRGFDDDTCGVLVRATLAAYLPPDRSWRNRLFDTV
                     ERGFAKVFFVRHFMGGDPAAAGRMGVKITAGDRALFLLVAVIVTSRMAAYGLALKIPG
                     LRTLADRDLVRQLRGHLARYGHAEFTTDAAQYRPTRPSAEPVAA"
BASE COUNT          205 a          493 c          428 g          234 t
ORIGIN      
        1 ggtaagggcc agggttgctg accagaagac acgccagccc gagctttatg gcggcatcga
       61 tttctcggcc acccccgagc gctttacgga tgatccggcc aacagcgtcg cccccgccag
      121 cgtggctggc gccgcgccca caccccagca catcgccctg gttcgcgcct acaccctgct
      181 gggcgaccgg gtggccgacg cctatgccgg cctgatgccg caacatggct tccggggcct
      241 gatcgccatg ctgaccaagg cctgcgacga aggccttgaa gccgtgcccg acgcgccgcc
      301 tgaactgatc gccttcattc gcgacatgga acatgtcccc gactggctgg acatggatct
      361 ggtgcgccag ggcgcgcggc tggaccgcaa cgccatggcc aacctgtcgc ccttcgccat
      421 ccgcggcgcg ttcgtcgcca ccttcatgaa caagtacgcg gccctgccca tggccctgac
      481 cgggaccctg ggggccgaga ccgccgcgcg ccgggtcaat gagacggcca gctttttcac
      541 caccaccttg ttgccgggcg ctcttgagcg ccatggcccc ggtttcaagg ctgccgctat
      601 ggtgcggttg atgcattcga tggtgcgctt caacgccctg cgccgacccg gcgcctggga
      661 cgcaaaggtc tatggcgtgc cgatccctca ggtcgaccag atgcccgccg gtctgatccc
      721 gatcttcctg ctcagctaca agctgttgcg acagggccgg accaccttta ccccggccga
      781 acgcgccagg gtcgaactgg cccgctatcg ctgctacctg ctgggcctgc ccgaggacct
      841 gctggccgac acgcccgtcg ggatcgtcca gaccatgagc gcccgcaacc agaccctgcg
      901 caggggcttc gacgacgaca cctgcggcgt gctggtccgc gcgaccctgg cggcctatct
      961 tccgccagac cggtcatggc gcaaccgcct gttcgacacg gtcgagcgcg gctttgccaa
     1021 ggtgttcttt gtccgtcatt tcatgggcgg cgatccggcg gcggcgggcc ggatgggcgt
     1081 gaagatcacc gcaggcgatc gggccctgtt cctgctggtc gcggtcatcg tcaccagccg
     1141 catggccgcc tatgggctgg cgctgaagat ccccggcctg cggacgctgg ccgaccgtga
     1201 cctggtgcgt cagctacggg ggcatctggc gcgatatggt catgcggagt tcacgaccga
     1261 cgcagcccag taccgcccta cccggccatc ggccgaaccg gttgccgcct agctggcgat
     1321 ctggaccagc ccaagtttca cccagacggc cttgtccttg
//
LOCUS       DAOMEO010001017         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9785_length_1360_cov_1.940230, whole genome shotgun sequence.
ACCESSION   DAOMEO010001017 DAOMEO010000000
VERSION     DAOMEO010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_1360_cov_1.940230"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLH31_17885"
     CDS             complement(<1..765)
                     /locus_tag="PLH31_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR91099.1"
                     /translation="MPLDVGARRKARVTSFDLAVDVARYDYADRIWSVRMAVRRALAD
                     YFIAERQAVAYQRVTQVRARQQAAMENRVNAGEASRAELERVRADGADAVRRAADAVN
                     SSRAARQSLATALGVPVEALNDVTLSWTDFDKPADDPGVGVTEAIRTAATLSRADVLK
                     AVAAYDQAESDLRAEVARQYPAISIAPGYTWERGLVKIPVSLGLVLPPLDLNRGAIAT
                     AQAKRTEAGRKLEAVVAGSLAAVDAALAETRAARDAL"
     assembly_gap    811..820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<821..1159)
                     /locus_tag="PLH31_17890"
     CDS             complement(<821..1159)
                     /locus_tag="PLH31_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91100.1"
                     /translation="MKSTLTVLLSALSLSACASYAPAPIEPSAFAAEARTRTLNLAQV
                     RERVASIAPDAGWSGPRVDRLALFASALLNNRDIAAARAGVDSAQAAAKASRVGPAAT
                     LTLSAEYARSA"
BASE COUNT          235 a          442 c          461 g          212 t
ORIGIN      
        1 cagcgcgtcg cgcgcagcgc gggtctcggc cagggccgca tcgacggccg ctagcgaccc
       61 cgcgacaacc gcttcgagct tgcgtcctgc ttcagtccgc ttggcctgcg ctgtggcgat
      121 agcgccgcga ttgagatcga gcggcggaag gacgaggccg agggataccg gaattttcac
      181 cagaccacgt tcccaggtgt agccgggcgc aatgctgatc gccggatact ggcgcgcgac
      241 ctctgcgcgg agatcggatt cggcctggtc ataggccgcc acggccttga gcacgtcagc
      301 gcgcgacaag gtggcggcgg tacgaatcgc ctccgtcaca cccacgcctg ggtcgtcggc
      361 cggcttatcg aagtcggtcc aggacagggt gacgtcgttc aaggcttcga ccggaactcc
      421 caaagccgtc gcgagtgact ggcgggccgc ccgagaactg ttgacggcgt ccgcggcgcg
      481 acgaacggcg tcggcgccat ccgcgcggac gcgctccagc tctgcgcggg acgcctcccc
      541 cgcgttgacc cggttttcca tcgccgcctg ttggcgcgcc cgcacctgtg tcacgcgctg
      601 gtaggcgacg gcctggcgtt ccgcgataaa atagtcggca agcgcccgcc gaacggccat
      661 gcgaaccgac cagatcctat cggcgtagtc gtagcgcgcg acatcgacgg caagatcaaa
      721 actggtcacc cgggccttgc ggcgagcgcc cacgtcgaga gggatgtcaa tcgccccgcc
      781 aaacagccag ggtgagcttt cggcggcgct nnnnnnnnnn ggcgctgcga gcgtattccg
      841 cacttaacgt gagtgtcgcg gcagggccga cgcgcgaagc cttggccgca gcctgggcac
      901 tatcgacgcc tgctcgagcg gcggcgatat cgcgattgtt gagcaacgcg ctggcgaaga
      961 gcgctaagcg atcgacgcgc gggccgctcc agcccgcgtc cggcgctatg gaggcgaccc
     1021 tttcccgaac ctgcgcgaga ttcaaagttc gggttcgagc ctccgcggcg aaggccgagg
     1081 gctcgatcgg tgccggcgcg taactcgcgc aggccgatag agacagggcg ctgagcagga
     1141 cggtcagagt cgacttcacg aggccaaagc tcccgcgacg ggcggtcgcg ggatagctta
     1201 tggctgcggt ctattgcgcc cgcaccttgt cgattcccaa acatggctgg ctcgatagag
     1261 ccaggagcgt gatcgagtcg ccgaatttca aagctgtgat gagcgcggcg atctggctga
     1321 acctgcctgg gaagtactcg ccccgaacat gaacagcagg
//
LOCUS       DAOMEO010001018         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9811_length_1357_cov_2.773425, whole genome shotgun sequence.
ACCESSION   DAOMEO010001018 DAOMEO010000000
VERSION     DAOMEO010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9811_length_1357_cov_2.773425"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLH31_17895"
     CDS             <1..314
                     /locus_tag="PLH31_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HQR91101.1"
                     /translation="GRPFALERRLISLKTAPGAATADFSAAPPGAWLLEHVAWTEAES
                     RISAVNADAEVAALLDLDEGVACLVVDRRTWRAGEHVTRVRQVFPGEAYDLVARFGPG
                     A"
     gene            370..615
                     /locus_tag="PLH31_17900"
     CDS             370..615
                     /locus_tag="PLH31_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91102.1"
                     /translation="MKTIVFTAAAAKDFDALPELARKAVERALDLYAITGQGDVRALQ
                     GRRGFRMRVRDYRVIFNEDVTTILAIYIGRRTTTTYR"
     gene            625..1008
                     /locus_tag="PLH31_17905"
     CDS             625..1008
                     /locus_tag="PLH31_17905"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91103.1"
                     /translation="MTAPQFILSPLGEELVVLARADYDSLVSAAGLLDDEDADDVAIY
                     DARMADVEGSQPLPVAVSAEVLKGSSLLKALRTWRGLSQVQLAQAAAIGQGFLSDLER
                     NAKRPGAETAEALAAALDVPVAWIR"
     gene            complement(1050..1126)
                     /locus_tag="PLH31_17910"
     tRNA            complement(1050..1126)
                     /locus_tag="PLH31_17910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1090..1092),aa:Met,seq:cat)
BASE COUNT          240 a          441 c          450 g          226 t
ORIGIN      
        1 gcggccggcc cttcgccctg gaacgccgcc tgatcagcct gaagaccgcc ccgggcgccg
       61 cgactgctga cttctcggct gcgccgccgg gcgcctggct gctggaacat gtcgcctgga
      121 ccgaagccga aagccgcatc agcgccgtca acgccgacgc cgaagtcgcc gccttgctcg
      181 acctcgacga aggcgtcgcc tgccttgtcg tcgaccgccg tacctggcgc gccggcgaac
      241 acgtcacccg cgtgcggcag gtgtttccgg gcgaggcgta tgacctggtg gcgcggtttg
      301 ggccgggggc ttgaggggct aagcgatatt ccatttgcga tttggtgata atctagcgat
      361 attatcgcca tgaagacgat tgtctttacc gccgctgccg ccaaggattt cgacgccctg
      421 cccgaactgg cccgcaaggc cgtcgaaagg gcgctcgacc tttatgccat caccggccag
      481 ggcgatgtac gggcgcttca gggccggcgg ggctttcgga tgcgggttcg agactatcgg
      541 gtgatcttca acgaggacgt caccaccatt ctggctatct acatcggccg ccgcaccaca
      601 accacctatc gatgaggagc cgcaatgacc gcaccgcaat tcattcttag ccctctggga
      661 gaggaactcg tggttctggc ccgcgccgac tacgacagcc tggtttcggc cgcgggcctc
      721 ctcgatgacg aggatgccga tgatgtggcg atctatgacg cgcgcatggc tgatgtagag
      781 ggaagccagc ccttgccagt ggcggtcagc gccgaagtcc tcaagggctc aagcctgctc
      841 aaggcgctgc ggacctggcg agggctcagc caggtgcaac tggcccaggc ggcggcgatc
      901 ggccagggat ttctctcgga tctcgaacgc aacgccaaac gcccgggcgc tgaaacagct
      961 gaagccctgg cggccgcgct ggacgtgccc gtcgcctgga tcaggtgatc cgccgacggg
     1021 gcgacaattg gaatgcggga aatccgtcct ggtggttgga ggcgggatcg aaccgccgac
     1081 ctgtgggtta tgaatccacc gctctaacca tctgagctac ccaaccgcac caggacggga
     1141 gcggggctta taggcgagaa gcgcgcggga tttcaagcga ccctgttcag gccacgatcg
     1201 acgccgccgt cgcgcccagg gctgcgacca gatcggcggg cgtcaggcgc atctgcaggc
     1261 cgcgctggcc accgttgacg aacacgtagg gctgggcgag ggcgcgctgc ccgcagacga
     1321 cgggctggcc ttgaaaagca gcagctacaa cgcctcc
//
LOCUS       DAOMEO010001019         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9813_length_1357_cov_2.200461, whole genome shotgun sequence.
ACCESSION   DAOMEO010001019 DAOMEO010000000
VERSION     DAOMEO010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9813_length_1357_cov_2.200461"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>647)
                     /locus_tag="PLH31_17915"
     CDS             complement(<1..>647)
                     /locus_tag="PLH31_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR91104.1"
                     /translation="IVTPKAVGKGKAAKIGLSFKDSRPAHAEEPKAPAGAPNVVVIIL
                     DDVGFADLGCYGGELQTPAMDALAGKGLRYTNFRTTAMCSCTRAALLTGLNHHSAGMG
                     WLADIDSGYPGYRGDLTRQNATLAEVLVEAGWSTFLLGKWHVNNADHGGPTGPFHNWP
                     TSRGFERAYWYQGHSSDHFHPGALYEGTAPVEAGGEGSYLSDDLAQRAATYLRTQ"
     assembly_gap    648..657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(714..1097)
                     /locus_tag="PLH31_17920"
     CDS             complement(714..1097)
                     /locus_tag="PLH31_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91105.1"
                     /translation="MRVVVRGLVGAVGVLGLLLAAMFLLRTEPAAAKFGLQALGPLGL
                     ASLRADMVALFGAVGILSLMGAVRDRGDLLLAPLILLGLALAGRMISLLMTGLSPDLI
                     PPMAVEVVLVAILALGHRVLARPQA"
BASE COUNT          237 a          469 c          431 g          210 t
ORIGIN      
        1 gctgggtgcg cagataggtc gcggcgcgct gcgccagatc atcggagagg taggagccct
       61 ccccgcccgc ctcgaccggg gccgtgcctt catagagggc gccggggtgg aagtggtcgc
      121 tggagtggcc ctgataccag taggcccgct cgaagccccg gctggtcggc cagttgtgga
      181 acggcccggt cggcccgccg tgatcggcgt tgttcacatg ccacttgccc agcaggaagg
      241 tcgaccaacc ggcctccacc agcacctcgg ccagggtggc gttctggcgc gtgagatcgc
      301 cgcgatagcc gggatagccg ctgtcgatat cggccagcca gcccatgccg gccgaatggt
      361 ggttcagccc cgtcagcagc gccgcccgcg tgcacgagca catggccgtg gtgcggaagt
      421 tggtatagcg cagccccttg ccggccaggg cgtccatcgc cggggtctga agctcgccgc
      481 catagcagcc caggtcggcg aacccgacgt cgtcgaggat gatcaccacg acattgggcg
      541 cgccggccgg ggccttgggc tcttcggcat gggccggccg cgagtccttg aacgacaggc
      601 cgatcttggc ggccttgccc ttgccgaccg ccttgggcgt gacgatcnnn nnnnnnnagc
      661 agcgagcgac ggtcgatcct gggcttcttg tcttcgtcca tgacagtccc cgatcaggct
      721 tgcggtcgcg ccaggacgcg atgtcccagg gccaggatcg cgaccaggac cacctcgacc
      781 gccatcggcg ggatcaggtc tggcgagaga ccggtcatca gaagcgagat catccgtccc
      841 gccagggcca ggccgagcag gatcagcggc gccagcagca ggtcgccgcg atcacgcaca
      901 gcgcccatca gcgacaggat gccgaccgcg ccgaacagcg ccaccatgtc agccctcaag
      961 gacgccagcc ccagcggccc gagcgcctgg aggccgaact tcgccgccgc aggctctgtc
     1021 cgcagcagaa acatcgccgc cagcaacagg cccagcaccc cgacggcgcc gaccagacct
     1081 cgcaccacaa cccgcatggt ttcgctcccc acctattatt gtggcatgct aactgccatt
     1141 tttatcggaa ggcaagcgtg gaccgcgccg aattagcgga tatagtcgca ttacaagtgc
     1201 cactatatcg aaacggccac acccgggagc gcgcgcatga gcctgaccta tgacctctac
     1261 tggtccttcc gctcgcccta ttcgtacatg atcacccatc ggctgacggc gctggagcgt
     1321 gagttcgacg tggtcgggaa catccgtcca gtctatc
//
LOCUS       DAOMEO010001020         1354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9846_length_1354_cov_3.095458, whole genome shotgun sequence.
ACCESSION   DAOMEO010001020 DAOMEO010000000
VERSION     DAOMEO010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9846_length_1354_cov_3.095458"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLH31_17925"
     CDS             <1..345
                     /locus_tag="PLH31_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR91106.1"
                     /translation="EPEAERFLDVLERNPSYISAVNYVETGLILIGRGQMATQEDLDE
                     WLKQLGAKVMPDENLAMAALSAYLIYGCRYHAAKLNLADCFAYALAKSLDAPLLYKGD
                     DFPLTDVRSALD"
     gene            complement(342..659)
                     /locus_tag="PLH31_17930"
     CDS             complement(342..659)
                     /locus_tag="PLH31_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQR91107.1"
                     /translation="MKFKIMYLEDKSKGLNGPARIGRVGSSKTGETLYYAGRSFEALR
                     GSGLKANFRDLETGQPFWIAKARKDGGDRLYKGAGDPPVIDDDVREEYWRDVRGVPDP
                     DLG"
     gene            complement(666..>1354)
                     /locus_tag="PLH31_17935"
     CDS             complement(666..>1354)
                     /locus_tag="PLH31_17935"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518874.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQR91108.1"
                     /translation="FWPGPLTLVAPIRDVSAVCDLARAGLDTVAIRVPGHPLSRAVLA
                     AFGGPVVAPSANRSGRPSPTTFEDAFSETGDKAAIALDGGPCEVGLESTVVAVLDGPP
                     RLLRPGAVTRAQLEAVVGPLAEAQDDAKRSPGRMALHYAPDAPVRIDATAPQAGEAFL
                     AFGPWPDGEGVFNLSPIGDLAQAAANLFSFLRAADRTRPVGIAVAAIPHEGLGEAIND
                     RLRRAAGFVG"
BASE COUNT          225 a          458 c          468 g          203 t
ORIGIN      
        1 gagcctgagg cagaacgctt tctggacgtt ctggagcgaa acccctccta catctcagcg
       61 gtgaactatg tcgagacggg gctgatcctg attggccggg gacagatggc cacccaagaa
      121 gacctggatg agtggctaaa acagttgggc gcgaaggtca tgcccgatga gaacctcgcg
      181 atggccgcgc tcagcgccta tctcatctac ggctgtcgct atcatgcagc gaaattgaac
      241 ctggccgact gtttcgccta cgccctggcc aagtcactgg acgccccgct gctctacaag
      301 ggcgacgact ttccgctgac ggacgtgcgc tccgcgctcg actaacccag gtcggggtcg
      361 ggaacgccgc gcacatcgcg ccagtattcc tcgcgcacgt catcgtcgat caccggcgga
      421 tcgcccgcgc ccttgtagag ccggtcgccg ccgtccttgc gggccttggc gatccagaag
      481 ggctggccgg tctccaggtc gcggaaattg gccttcagcc ccgagccgcg cagggcctcg
      541 aacgaacggc cggcgtaata gagggtctcg ccggtcttgg acgagcccac ccggccgatg
      601 cgggcgggac cgttcaggcc cttggacttg tcctcgaggt acatgatctt gaatttcatc
      661 ggcctctatc ccacgaaccc ggccgcgcgt ctcagccggt cattgatcgc ctcgcccagg
      721 ccctcgtgag ggatggccgc cacggcgatg ccgaccggcc gggtccgatc ggcggcgcgc
      781 aggaacgaaa agagattggc cgcggcctgg gccaggtcgc cgatcggact gagattgaac
      841 accccctccc cgtccggcca cggaccaaag gccaggaacg cctcgccagc ctgcggcgcg
      901 gtcgcgtcga tccgtaccgg cgcgtcgggg gcatagtgca gggccatccg gccgggggag
      961 cgcttggcgt cgtcctgcgc ctcggccaag gggcccacca ccgcctccag ctgggcgcgg
     1021 gtcacggcgc cggggcgcag caaacgtgga ggaccgtcca gaacggcgac gacggtggat
     1081 tccaggccga cctcgcaagg cccgccgtcg agggcgatgg cggccttgtc gcccgtttcg
     1141 ctgaacgcgt cttcgaaggt cgtggggctt ggccggcccg agcggttggc tgacggcgcc
     1201 acgacgggtc cgccgaacgc cgccagaacg gcgcgggaca gcggatggcc gggcacgcgg
     1261 atcgccacgg tgtcgagacc ggcccgggcc aggtcgcaga ccgcgcttac gtcacggatc
     1321 ggggcgacga gggtcaaagg gccgggccag aagg
//
LOCUS       DAOMEO010001021         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9854_length_1353_cov_2.660247, whole genome shotgun sequence.
ACCESSION   DAOMEO010001021 DAOMEO010000000
VERSION     DAOMEO010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9854_length_1353_cov_2.660247"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1292
                     /locus_tag="PLH31_17940"
     CDS             81..1292
                     /locus_tag="PLH31_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR91109.1"
                     /translation="MTQAAARPALIVVAASFGFLVTQLDVTIVNVALDAIGAAFDAPM
                     AALQWVVDAYVLALAAMLLSAGALGDRIGPRRVFVAGLVLFGLASAACGLAAGPGALI
                     AARAAQGGLLVAALDWRWVFLVNLPLCAIGVALTLAVTPEAPPRARKPLDLPGQALAL
                     IAVTALVGAVIEGGRRGWSHPGPLVALVVGALAVAAFLWVEARSPHAVAPLSMFRQQS
                     VSTAVLVGSALNFTYYGLVFVLGLYLQRAVGYGPEQAGLAFLPLTATFILSNLISGGL
                     VARFGAARVTGAGVLIAAGGYALTARLGAGSSFWEMLPGFALIPGGMGLAIPALTSGL
                     LASVDRSVAGAATGVLNACRQAGGAAGVAVFGALAAGGPERIVSGLRVSGVVAAVMLV
                     GAAWVVGRRVR"
BASE COUNT          125 a          431 c          556 g          241 t
ORIGIN      
        1 gacatcctac cccagcatgg accgcgatgg ttcatcctcg cctgtagcat caagcgaccg
       61 gtcgaaggca tgacagcgcc atgacccaag ccgccgcccg tcccgccctg atcgtcgtcg
      121 ccgccagttt cggctttctg gtcacccagc tggacgtgac catcgtcaat gtggccctgg
      181 acgccatcgg ggcggcgttc gatgcgccga tggcggcgtt gcaatgggtg gtcgacgcct
      241 atgtgctggc cctggcggcc atgctgctgt cggccggggc cctgggcgac cggatcgggc
      301 cccggcgggt gttcgtggcg gggctggtgc tgtttggcct ggcctcggcc gcctgcggac
      361 tggcggcggg gccgggcgcc ctgatcgcgg cgcgggcggc gcagggcggc ctgctggtgg
      421 cggcgctgga ctggcgctgg gtattcctgg tcaatctgcc gctgtgcgcg atcggcgtgg
      481 ccctgaccct ggccgtcacc ccggaggcgc cgcccagggc ccgcaagccg ctggacctgc
      541 ccggacaggc cctggccctg atcgcggtga cggccctggt cggggcggtg atcgagggcg
      601 gccggcgggg ctggagccat ccggggccgc tggtcgcgct ggtcgtcggg gcgctggcgg
      661 tggccgcctt cctgtgggtc gaggcgcgca gtccgcatgc ggtggcgccg ctgtcgatgt
      721 ttcgccagca gtcggtgtcg acggcggtgc tggtcgggtc ggcgctgaac tttacctatt
      781 acggcctggt cttcgtgctg gggctctatc tgcagcgggc cgtcggctat gggcccgagc
      841 aggcgggcct ggccttcctg cccctgacgg cgaccttcat cctttccaac ctgatcagcg
      901 gcggactggt cgcgcggttc ggcgctgccc gggtgaccgg cgccggggtg ctgatcgcgg
      961 ccggcgggta tgccctgacc gcgcggttgg gcgcagggtc gagtttctgg gagatgctgc
     1021 cgggctttgc cctgatcccg ggcgggatgg ggctggcgat cccggccctg acgtccggat
     1081 tgctggccag cgtcgaccgc agcgtggccg gggcggcgac gggggtcctg aacgcctgcc
     1141 gtcaggccgg cggcgcggcc ggggtggcgg tgttcggcgc gctggcggcg ggcgggccgg
     1201 agcggatcgt cagtgggctg agagtgtcag gtgtggtggc ggcggtgatg ctggttgggg
     1261 cggcctgggt ggtggggcgg cgcgttcgtt agccttctcc cttccccctt gatgggggaa
     1321 gggccgggga tgggggtgac gcagcggtgc agc
//
LOCUS       DAOMEO010001022         1353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9856_length_1353_cov_1.735747, whole genome shotgun sequence.
ACCESSION   DAOMEO010001022 DAOMEO010000000
VERSION     DAOMEO010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9856_length_1353_cov_1.735747"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>852
                     /locus_tag="PLH31_17945"
     CDS             <1..>852
                     /locus_tag="PLH31_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQR91110.1"
                     /translation="DRRFSEVYAGAGLASASSGDSLDLDGGLGLLLEDIPAAADLLDP
                     QDDAPIIRLINGLIAEAVRLGASDIHIEPFEQALIVRLRIDGLLREVLSLSPRIAAML
                     VSRVKVMAKLDIAEKRLPQDGRISLALGGKSLDVRVSTLPARMGERVVMRILDKDQAG
                     LRLADLGMSPEVLSALEAAVREPHGIILVTGPTGSGKTTTLYAALAVLNDNSRNILTI
                     EDPVEYAVHGIGQTQVNTKVGMTFAAGLRAILRQDPDVVMVGEIRDSETAQIAVQASL
                     TGHLVLST"
     assembly_gap    853..862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <863..1307
                     /locus_tag="PLH31_17950"
     CDS             <863..1307
                     /locus_tag="PLH31_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQR91111.1"
                     /translation="HTNDAAGAVVRLRDMGVEPFLLASTLRLIIAQRLVRRLCEHCRT
                     PEPADAAAAALVGIEPGETLYRPVGCSHCAHTGYQGRIGIYEALRVDDRLRRLIGGNA
                     TEQEITAAGLDRDLGQEARRYILAGLTTVEEALRITGQGARDGDL"
BASE COUNT          200 a          475 c          454 g          214 t
ORIGIN      
        1 gatcggcggt tctccgaggt ctatgccggc gcgggactgg cctcggcgtc cagcggcgat
       61 agcctggatc tggacggcgg tcttggcctg ctgctggaag atatcccggc ggcggcggat
      121 ctgctggatc cccaggacga cgcgccgatc atccggctga tcaacggcct gatcgccgaa
      181 gccgtgcgcc tgggcgcctc cgacatccat attgagccct tcgagcaggc cctgatcgtg
      241 cgcctgcgca tcgacggcct gttgcgcgaa gtgctgagtc tgtcgccgcg catcgccgcc
      301 atgctggtgt cgcgcgtcaa ggtgatggcc aagctcgaca tcgccgagaa gcgcctgccc
      361 caggatggcc gaatctcgct ggccctcggt ggcaagagcc tcgacgtgcg ggtctccacc
      421 ctgccggccc gcatgggcga gcgggtggtg atgcggatcc tcgacaagga ccaggcgggc
      481 ctgcgcctgg ccgacctggg catgtcgccc gaggttctga gcgctctgga ggccgctgtg
      541 cgcgagcctc atggcatcat cctggtcacc ggcccgaccg gctcgggcaa gacgacgacg
      601 ctctatgccg ccctggcggt gctgaacgac aacagccgca acatcctgac gatcgaagac
      661 ccggtcgaat acgccgtgca tggcatcggc cagacccagg tcaacaccaa ggttggcatg
      721 accttcgcgg ccggcctgcg ggccatcctg cgccaggacc ccgatgtggt gatggtcggt
      781 gaaatccgcg attccgagac cgcccagatc gcggtccagg ccagcctgac gggccacctg
      841 gtgctgtcga ccnnnnnnnn nnccacacca acgacgcggc cggcgcggtc gtgcgtctgc
      901 gcgacatggg cgtcgagccc ttcctgctgg cctcgaccct gcggctgatc atcgcccagc
      961 gcctggtgcg ccgcctgtgc gagcattgcc ggacgcccga gcctgcggac gcggccgccg
     1021 ccgccctggt cggcatcgag ccgggcgaga ccctctatcg ccctgtcggc tgctcccact
     1081 gcgcccatac cggctatcag ggccggatcg gcatctacga ggccctgcgc gtcgacgacc
     1141 gcctgcgccg tctgatcggc ggcaacgcca ccgagcagga gatcacggcc gctggcctgg
     1201 atcgcgatct gggccaggaa gcccgccgct acatcctagc cggtctgacg acggtcgagg
     1261 aggcgcttcg gatcaccggg cagggggctc gtgatggaga cctttgacta tgtcgccctc
     1321 gatctggcgg gacacacccg tcgcggccag ctg
//
LOCUS       DAOMEO010001023         1351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9870_length_1351_cov_2.597994, whole genome shotgun sequence.
ACCESSION   DAOMEO010001023 DAOMEO010000000
VERSION     DAOMEO010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_1351_cov_2.597994"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLH31_17955"
     CDS             complement(<1..1309)
                     /locus_tag="PLH31_17955"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQR91112.1"
                     /translation="MAGSGSLAQVGETYALSLDAAGRMGRTDIKTREAARVRFGGVST
                     VARLRLLIGDGKADLDADLGGQSASLRARLDGVSLTAFDPDLAGDIDGTFNLNGQGET
                     LLGDFAAKLTGARERGSKIEQSLDAQIQGQLKDRVLDIVANGANRQGLKAEARLQLPA
                     EASAKPLRLAINRTRPVMGTFKAQGEIKPLWDLLLGPERSLSGKIDAEGTLAGTLADP
                     RLVGRAALDDGGFEEGQTGLRLRKITLRMQLADNAIDVSEATAEDGGSGSVTGGGRIS
                     LNREGVSSFKLNLAGFQLIDNDLATATATGQATVNRSADGKVKLSGDLTIDRADVSAQ
                     SKTPAGVVNMDVIERNRPTELDQGAQPRRPSPGGGIALDINLTAPRRVFVKGRGLDVE
                     LSLDAHVGGTTFAPRLDGVARMVRGEYDFAGKRFEFDDNGVDTL"
BASE COUNT          187 a          497 c          438 g          229 t
ORIGIN      
        1 acagcgtgtc cacgccgttg tcgtcgaact caaagcgctt gccggcgaag tcgtactcgc
       61 cgcgcaccat ccgcgccacg ccgtccaggc gcggggcaaa cgtcgttccg ccgacatggg
      121 cgtccagcga cagctcgacg tccagccccc ggcccttcac gaacacgcgg cgtggcgcgg
      181 tcaggttgat atccagtgcg atgccgccgc cggggctggg tcggcgcggc tgcgcgccct
      241 ggtccagttc cgtgggtcga ttgcgttcga tgacatccat gttgacgaca ccggccgggg
      301 tcttgctttg agccgagacg tcggcgcggt cgatggtcag gtcgccgctc agcttgacct
      361 tgccgtcggc cgagcggttg acggtggcct gtccggtggc ggtcgccgtc gccaggtcgt
      421 tgtcgatcag ctggaaaccg gcaagattca gcttgaagct gctgacaccc tctcgattga
      481 ggctgatgcg ccccccgccc gtgaccgacc cgctcccacc atcctcggcc gtggcctcgc
      541 tcacatcgat ggcgttatca gccagttgca tccgcaaggt aatcttgcgc agccgcagcc
      601 ccgtctggcc ctcttcgaac ccgccgtcgt cgagcgcggc ccggcctacc agccgaggat
      661 cggccagggt gcccgccagc gtgccttcgg cgtcgatctt gcccgaaagc gagcgctccg
      721 gacccagcaa caggtcccat agaggcttga tctcgccctg ggccttgaag gttcccatca
      781 ccggccgcgt gcgattgatc gccagacgaa gcggcttggc cgaggcctcg gccggcagct
      841 gcagccgggc ttcggccttc aggccctgac ggttggcgcc attggccacg atgtccagca
      901 cgcggtcctt cagctggccc tggatctggg cgtccagcga ctgctcgatc ttcgagccgc
      961 gctcccgcgc gcccgtcagc ttggccgcga agtcgcccag cagcgtctcg ccctggccgt
     1021 tcagattgaa ggtcccgtcg atgtcgcctg caaggtcggg atcgaaagcc gtaaggctca
     1081 caccgtccag ccgcgccctt aggctggcgc tctggccgcc caggtcggcg tcgaggtcgg
     1141 ccttgccgtc gccgatcagc aagcgcagac gcgcgacggt cgagaccccg ccgaagcgca
     1201 cccgcgccgc ttcgcgggtc ttgatgtcgg tccgtcccat acgcccggcg gcgtccagcg
     1261 acagagcgta ggtctcgccg acttgcgcta ggctccccga gccagccacg cgccagcgcc
     1321 cgccgggcgc cacgccctgg ccgtcgacgg t
//
LOCUS       DAOMEO010001024         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9886_length_1350_cov_2.511969, whole genome shotgun sequence.
ACCESSION   DAOMEO010001024 DAOMEO010000000
VERSION     DAOMEO010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_1350_cov_2.511969"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..714
                     /locus_tag="PLH31_17960"
     CDS             181..714
                     /locus_tag="PLH31_17960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HQR91113.1"
                     /translation="MKILGVSGSLRAASFNTALLRAAQQLAPASVSIEIFGLHDLPLF
                     NQDVEDQGDPPAVTAWKDAIRGAEALLLACPEYNGGVTGVLKNAIDWASRGKPAPLAG
                     KICCLVGASPGATGTVRAQDALRLSLRRAGAEAVPLGEILVGQAHTKIVDGVLADETT
                     RSYLARHLASFAARLRG"
     gene            complement(711..>946)
                     /locus_tag="PLH31_17965"
     CDS             complement(711..>946)
                     /locus_tag="PLH31_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91114.1"
                     /translation="GARIQQSVAPYDAAMQGSVKIGGEWAMHTVPGKAAIDIPAGGGN
                     VSLHLASARQTVVLGPVFVLDFGPDYDLSKLAN"
     assembly_gap    947..956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<957..1275)
                     /locus_tag="PLH31_17970"
     CDS             complement(<957..1275)
                     /locus_tag="PLH31_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91115.1"
                     /translation="MKAVIVAASLLALAAGAGSSVLARQDKDVDILQKAINKPSANWQ
                     VQGAEQTSKPVKDKTVVGGGGVRVEVAAASSQPWAVAAQQPISGKIARGDTLLLAFWA
                     KAES"
BASE COUNT          205 a          476 c          426 g          233 t
ORIGIN      
        1 ggccgctcgg tctgcgaatg ggaccccggc tcggccgcca gccaggaggt cgagcgcctg
       61 tggagctata tcgtcccgat gctgcacggt caggccgatc tgcagacccg cgcaagctga
      121 tcggcttggc gtcagcgatc gaacacccct aagccttccg catccctgtc ggaggtccgc
      181 atgaagattc ttggcgtgtc tggcagcctg cgggccgcct cgttcaacac cgctctcctg
      241 cgggccgcac agcagctggc gcctgccagc gtttccatcg agatcttcgg gctgcacgat
      301 ctgccgctct tcaatcagga tgtggaggac cagggcgatc cgccagccgt caccgcctgg
      361 aaggacgcca ttcggggcgc ggaagccctg ttgctggcct gccccgagta taacggcggc
      421 gtcaccggcg tgcttaagaa cgccatcgat tgggcctcac ggggaaaacc cgccccgctg
      481 gcgggcaaga tctgttgtct ggtcggcgcc agccctggcg cgactggaac cgtccgcgcc
      541 caggacgccc tgcgcttgtc tcttcgtcgc gccggggccg aggcggtccc gttgggcgag
      601 attctggtcg gccaggcgca caccaagatc gtcgatggcg ttctggccga cgagaccacc
      661 cgaagctatc tggcccgcca cctggcgagc ttcgccgccc ggcttcgcgg ctagttggcc
      721 agtttcgaca aatcgtagtc cggtccgaag tccagcacga acaccggtcc cagcaccacc
      781 gtctgcctgg ccgaagccag gtgcagcgag acgttgccgc cgcccgctgg aatatcgatc
      841 gcagcctttc ctggcacggt atgcatcgcc cactcgccac cgatcttgac cgagccctgc
      901 atggcggcgt catagggggc gacggactgc tggatacgcg cgcccgnnnn nnnnnncgct
      961 ttcggccttg gcccagaagg ccagcagcag ggtgtcgccc ctggcgatct tgccgctgat
     1021 cggctgctgg gcggccacgg cccaaggctg agaactggcg gcggcgacct cgacacgaac
     1081 gccgccgcca ccgactacgg tcttgtcctt cacaggcttg ctggtctgtt cggcgccttg
     1141 cacctgccag ttggccgagg gcttgttgat cgccttctgc aggatgtcga catccttatc
     1201 ctgccgcgcc agaacggacg acccggcgcc ggcggccagc gccagaagcg acgcggccac
     1261 gatgacagct ttcatacggc tctctcccct tgttccaaca gaacggaggc gtcccctgga
     1321 cggctccggc gcttcccccc agggctggcc
//
LOCUS       DAOMEO010001025         1349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9897_length_1349_cov_2.283617, whole genome shotgun sequence.
ACCESSION   DAOMEO010001025 DAOMEO010000000
VERSION     DAOMEO010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9897_length_1349_cov_2.283617"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1242
                     /gene="bioF"
                     /locus_tag="PLH31_17975"
     CDS             97..1242
                     /gene="bioF"
                     /locus_tag="PLH31_17975"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQR91116.1"
                     /translation="MQSLDAFAGEKLTALEAASLRRILKPTRRLDGARVERDGRQLIS
                     FSCNDYLNLSHHPAVRAAAAEAALNYGAGAAASRLVTGDHPLLGDLEKRLARLKGTEA
                     ACVFGSGYLANTGVIPTLVGPDDLILVDELAHACIWAGAKLSGARTTAFKHNDVADLE
                     RLLQAERGSARHVLVATDGVFSMDGDIAPLDQLSDLCAAHEAWLLSDDAHGVGVLAGG
                     RGSAALFPDAEIPLQMGTLSKALGSFGGYLCGSQAVIDLLKTRARTLVYATGLPPASA
                     AAALAALDIVETQPALTALPLAKARAFTRALDLPLAQSPIVPVIIGSAEAALAASARL
                     EVEGYLVVAIRPPTVPDGAARLRIAFSAAHSDADVARLADLVADLRR"
     gene            1239..>1349
                     /locus_tag="PLH31_17980"
     CDS             1239..>1349
                     /locus_tag="PLH31_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR91117.1"
                     /translation="MRSLFVAGTGTDIGKTHVACALIRAVRARGLSVDAFK"
BASE COUNT          183 a          498 c          444 g          224 t
ORIGIN      
        1 gggggtcatg gcgatccttc cttgcgccac cttttcgttg cgcagcgggg gcgccttgcc
       61 tatgccgtgc gccctagctt cgcaaagggg aaacacatgc agagcctcga cgccttcgcg
      121 ggcgaaaagt tgaccgccct cgaagctgca agcctgcgcc gcattctgaa gcccacccgc
      181 cggctggacg gcgcgcgggt cgaacgcgac ggccggcagc tgatttcgtt ctcgtgcaac
      241 gactatctga acctgtcgca ccatcccgcc gtccgcgccg ccgccgccga ggccgccctg
      301 aactatggcg ccggcgctgc cgcctcgcgg ctggtcactg gcgatcatcc gctgctgggc
      361 gatctggaaa aacgcctggc gcgcctgaaa ggcacagagg ccgcttgcgt gttcggctcg
      421 ggctatctgg ccaataccgg cgtcatccca acgctggtcg ggcccgacga cctgatcctg
      481 gtcgacgaac tggcccatgc ctgcatctgg gccggggcga agctgtcggg cgctcggacc
      541 accgccttca agcacaatga cgtcgccgat ctggagcgtc tgctgcaggc cgagcgcggc
      601 tcagcccgcc acgtcctggt cgccaccgac ggcgtcttct cgatggacgg cgacatcgcg
      661 cctctcgacc agctgtcgga cctttgcgcg gcgcacgagg cctggctgct gtcggacgac
      721 gctcatgggg tgggcgtcct ggcaggggga cgcggctcgg cggcgctgtt ccccgacgcc
      781 gaaatcccgc tgcagatggg cactctgtcc aaggcgctcg gctcgttcgg cggctacctc
      841 tgcgggtcgc aggcggtgat cgatctgctg aaaacccggg ctcgcaccct ggtctacgcc
      901 accggcctgc ccccagcctc ggcggcggcg gcgctggcgg cgctcgacat cgttgaaacc
      961 cagcctgcgc tgaccgcctt gcccctggcc aaggctcgcg ctttcacccg cgccctggac
     1021 ttgccgctgg cgcaaagccc gatcgtaccg gtcatcatcg gctcagccga agccgcgctg
     1081 gcggcctcgg cgcggcttga agtcgagggc tatctcgtcg tcgccatccg cccgccaacg
     1141 gtgccggacg gcgcggcgcg cctgcggatc gccttcagcg ccgcccattc ggacgccgac
     1201 gtcgcgcgac tggccgatct ggtcgcggac ctccgtcgat gaggtcgctg ttcgtcgccg
     1261 gcaccggcac cgacatcggc aagacccatg tcgcctgcgc cttgatccgc gccgtccgcg
     1321 cccgggggct gagcgttgac gccttcaag
//
LOCUS       DAOMEO010001026         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9908_length_1348_cov_1.433101, whole genome shotgun sequence.
ACCESSION   DAOMEO010001026 DAOMEO010000000
VERSION     DAOMEO010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_1348_cov_1.433101"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLH31_17985"
     CDS             complement(<1..293)
                     /locus_tag="PLH31_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91118.1"
                     /translation="MLIELRKQKIIKHLSQDCSCYTAELWIDGRLAFLASNRGQGGAD
                     DYRQVGVITEAEVDTWLKANRPVRPFDGIVLAPTLEHEVAFLMDEAENIAMLRR"
     gene            complement(610..>1348)
                     /locus_tag="PLH31_17990"
     CDS             complement(610..>1348)
                     /locus_tag="PLH31_17990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRTRC system ThiF family protein"
                     /protein_id="HQR91119.1"
                     /translation="QMLMGLASLDTALRAISTRSLHVTVVDDDVVSEANLGRQPFYRC
                     DLGSSKAHTLTERINLAHGLAWKAVHGRAPGAIDISKADILITCVDTASARRALGAAM
                     ATGSTPPTYWMDLGNRASDGQFILGCPGREHGQDRRRLPTVLEYFPEIADERLPDDNS
                     PSCSVAEALERQSLFVNRVLASHALALFFDLIGRGSIGHAGAFINLASGQCVPIPLRA
                     TDGTIDDRGSAVELDESAARPLIGAQR"
BASE COUNT          250 a          391 c          468 g          239 t
ORIGIN      
        1 cggcgcagca ttgcgatgtt ctcggcctcg tccatgagga aggccacttc atgttcgagc
       61 gtgggcgcca gtacgatgcc gtcgaatggc cgaacaggtc gattggcctt caaccaggta
      121 tcaacctcgg cctcggtgat gacgccgacc tgacgatagt catcggcgcc gccctgtccg
      181 cgattgctgg cgaggaacgc caaccggcca tcgatccaca gctccgccgt atagcaggag
      241 caatcctggg ataggtgctt gatgatcttc tgtttgcgga gctcaatcaa catcgggtga
      301 tctccttgga ggacagtgca gcgggcgccg gaggcatctt gggaaccccg gttcgttgtc
      361 ggcggccagg ccccgggccg atggcgcgag caatgtgtcc cgcatagact ccgcacgcgg
      421 cccaataggc cgcgaccgga ggctttcgat tccgccagct gcgttccgcg cgcaaacgag
      481 catcgttctg gatgtcgagc aggatgtcct ggagggcgag acgggcgtca ggactgaggg
      541 cgccaagccg agcgaccgac gggagggtga gaatcggatt gcggacctcg gccttggagg
      601 aacgcacgct catcgctggg ctccgatcag gggcctcgca gcgctttcgt ccagctcaac
      661 ggctgacccg cgatcgtcga tcgttccatc ggtcgctcgc agcggaatcg ggacgcattg
      721 tccgctggcg agatttatga aagcgccggc gtggccgatg gagccgcggc cgatcaaatc
      781 aaaaaacagg gcgagagcgt ggctggccag aacgcggttc acgaagaggg attgccgttc
      841 gagggcctcg gcgaccgagc aggatgggct gttgtcgtcc ggaaggcgct catcagcgat
      901 ctcggggaaa tattcgagga cggtgggaag gcgtctgcgg tcctgcccgt gctcgcggcc
      961 agggcagccg agaatgaact gaccgtcgct ggcgcggttg ccgaggtcca tccaataggt
     1021 cggtggcgtg gatcccgtcg ccattgcagc gccaagcgcg cggcgcgcgg acgccgtgtc
     1081 gacgcaggtg atgaggatat cggctttcga gatatcgatc gcgcctggcg cgcggccgtg
     1141 aacagccttc caggcaagac catgcgcgag gttgatccgc tcggtgagtg tatgggcctt
     1201 cgagctgccg aggtcgcatc ggtagaatgg ttgtcgacca agattggctt cgctgacgac
     1261 gtcatcgtcg acgacagtga catggagcga tcgggtggaa atagcgcgca gcgccgtgtc
     1321 gagcgaggca agacccatca gcatctgc
//
LOCUS       DAOMEO010001027         1347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9911_length_1347_cov_1.962074, whole genome shotgun sequence.
ACCESSION   DAOMEO010001027 DAOMEO010000000
VERSION     DAOMEO010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_1347_cov_1.962074"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLH31_17995"
     CDS             <1..304
                     /locus_tag="PLH31_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR91120.1"
                     /translation="LELKSSLEIARRRCQPVVVTADIRAQGVASVGMEVMFAPLRGVN
                     GETDRFLGIYQPIAMMARLEGRPAYELGVRQIQSLGLADQDGPQLRLATLNGRRIA"
     gene            complement(301..732)
                     /locus_tag="PLH31_18000"
     CDS             complement(301..732)
                     /locus_tag="PLH31_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HQR91121.1"
                     /translation="MRADLIERSFEIAAERCGDLTPLVYARLFDQQPAMEPLFCLDTH
                     HQVRGEMLSQVIRAMLDFIGERQYAATLIQTEVMNHAGYDVPPAVFRTFFDVVAETVR
                     DLMAEDWTDEVDAAWRELLTALDYYVTHPDQAETARDAAPA"
     gene            complement(738..>1347)
                     /locus_tag="PLH31_18005"
     CDS             complement(738..>1347)
                     /locus_tag="PLH31_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR91122.1"
                     /translation="QTDYIDLYQSHRDDADTPVEETLEAYGRLVKAGKVRAIGASNFT
                     PARLGESLTASAAGGLPRYETIQPKFNLYDRDEVEGELAALTAAEGLGVIPFYGLAAG
                     FLTGKYRTPADLKGRARAGTIKRDYLNDKGLQVLAALDKAAAATGATQAQLALAWIMA
                     HPAVTAPIASATSVAQLKELMAAAQLVLPTGIIADLNAAGRA"
BASE COUNT          214 a          459 c          441 g          233 t
ORIGIN      
        1 gctggagctg aaatcttcgc tggagatcgc ccgtcgccgc tgccaaccgg tcgtggtcac
       61 cgccgatatc cgggcgcaag gcgttgcatc tgtcggcatg gaggtgatgt ttgctcctct
      121 gcgcggggtg aatggcgaaa ctgaccggtt cctgggcatc tatcagccga tcgccatgat
      181 ggcgcggctt gagggtcgtc cggcctacga actgggcgtg cgccagatcc agtcgctggg
      241 cctggccgat caggacggcc cgcagctgcg cctggccacg ctgaacggcc gccgcatcgc
      301 ctaagccggc gcggcgtccc gcgcagtctc ggcctggtcc ggatgcgtga catagtagtc
      361 gagcgccgtc agcagctctc gccatgcggc gtcgacctcg tcggtccagt cctcggccat
      421 cagatcgcgc acagtctcgg cgaccacgtc gaagaaggtg cggaaaacgg ccggcggcac
      481 gtcatagccg gcatggttca tcacttcggt ctggatcagc gtggccgcgt actgccgctc
      541 gccgataaaa tccagcatgg cgcggatcac ctgcgacagc atctcgccgc gaacctgatg
      601 atgggtgtcc aggcagaaca gcggctccat ggcgggctgc tggtcgaaca gccgggcata
      661 gaccagcggg gtcaggtccc cgcaacgttc ggcggcgatc tcgaagctgc gctcgatgag
      721 gtcggcccgc accggcccta ggcccgcccg gccgcgttca ggtcggcgat gatcccggtg
      781 ggcaggacca actgggccgc agccatcagt tccttcagtt gcgcgaccga ggtcgcgctg
      841 gcgatcggcg ccgtcaccgc cggatgcgcc atgatccagg caagggccag ctgggcctgg
      901 gtcgcgcccg tcgccgccgc ggccttgtcc agagccgcca gaacctgcaa acccttgtcg
      961 ttgagataat cgcgcttgat cgtccccgcc cgcgcccgcc ctttcaggtc agccggggtt
     1021 ctgtacttgc ccgtcagaaa tccggcggcc agaccataga aggggatgac gcccagcccc
     1081 tcggccgctg tcagcgccgc cagttcgccc tcaacctcat cacggtcgta caggttgaac
     1141 ttcggctgga tcgtctcata gcgcggcaag ccgcccgcgg ccgacgccgt gagactttcg
     1201 ccgagccggg ccggcgtaaa gttggatgcg ccgatagccc gaaccttgcc ggcttttacg
     1261 agccggccat aagcctccag agtctcctcg accggcgtat ccgcgtcgtc ccggtgcgac
     1321 tggtagaggt cgatatagtc ggtctgc
//
LOCUS       DAOMEO010001028         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9927_length_1345_cov_2.696899, whole genome shotgun sequence.
ACCESSION   DAOMEO010001028 DAOMEO010000000
VERSION     DAOMEO010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_1345_cov_2.696899"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..736)
                     /gene="raiA"
                     /locus_tag="PLH31_18010"
     CDS             complement(104..736)
                     /gene="raiA"
                     /locus_tag="PLH31_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQR91123.1"
                     /translation="MQVQISGKHVDVGEALRARVSDEITLSIGKYFDRGGNADVVISK
                     EGHAFRADCSVKLASGQQLISHGTGGDAHAAFDAALAKIETRIRRYKRRLKSHSIAAS
                     AKQAENAAMYVLKAPDIDEHEDDDWEGDEAHPAGAPSAMIIAETQASLKVMTVSMAVM
                     ELDLTESQAIVFRNAAHDGLSVVYRRPDGNIGWIDPERTKSLNGNGSTAN"
     gene            complement(787..>1345)
                     /locus_tag="PLH31_18015"
     CDS             complement(787..>1345)
                     /locus_tag="PLH31_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HQR91124.1"
                     /translation="PSLTVGEMLDAFDSRAFGAMLFVFGALNTLPLPPGSSTILSLPI
                     LLLAPQVALGADAPWLPRRMIERPLKRDDLRGLFRKLTPIVKWMELVTRPRLTWIFTP
                     LGERMIGVVCTLLALVLILPIPLGNLAPGATVAILSLALLQRDGLLALLGYLMAFVSL
                     GLLFVSAGVVTAAVREMIGLVSGFF"
BASE COUNT          257 a          426 c          424 g          238 t
ORIGIN      
        1 gagatcgccg atagtcatgg acttaaacgc tcgctcgctg tcggccaccg caacgccatc
       61 gctggcggtc ctccggaacg ttcaggatcc tgacaggatc ccgttagttc gcggttgacc
      121 cgttcccgtt cagcgacttg gtgcgttcgg ggtcgatcca gccgatgttt ccgtctggcc
      181 gccgatacac cacggacaat ccgtcatggg cggcgtttct aaacacgatt gcctgcgact
      241 cggtcaagtc cagttccatg acagccatgg aaacagtcat caccttcaga gacgcctggg
      301 tctcggcgat gatcatggcc gagggcgcgc ctgcgggatg agcctcatca ccctcccagt
      361 catcatcctc gtgctcatcg atgtcgggcg ctttcagcac gtacatcgcc gcgttctcgg
      421 cctgtttggc cgaggcggcg atggagtgac ttttcagtcg tcgcttatag cgcctgatac
      481 gcgtctcgat cttggccagg gcggcgtcaa aggccgcgtg ggcgtcaccg cccgtaccgt
      541 ggctgatcag ttgctggccc gaagccagct ttaccgaaca gtcggcccgg aaggcatggc
      601 cctccttgct gatgaccacg tcggcgttgc cgccgcgatc aaaatacttg ccgatgctca
      661 gggtgatttc gtcggagact cgcgctcgca gagcctcgcc aacgtcgaca tgcttgccgg
      721 agatttggac ttgcataccg taacaacgcg cctgtcgcgg gtcggtgtca agcggcgccg
      781 aagggttcag aaaaagcctg aaaccagacc gatcatttca cgaacggcgg cggtcaccac
      841 ccccgcgctg acgaacagca ggcccaggct gacaaaggcc atcagatagc ccagcagggc
      901 cagaagcccg tccctttgca gcaacgccag cgacaggatg gcgaccgttg cgcccggcgc
      961 cagattgccc aggggaatgg gcaggatcag caccagggcc agcagggtgc agaccacgcc
     1021 gatcatccgt tcgcccagcg gcgtgaagat ccaggtcagg cggggccggg tcaccagttc
     1081 catccatttg acgatcggag tcagtttgcg aaacaggccc cgcaggtcat cgcgcttcag
     1141 gggccgctcg atcatcctgc ggggcagcca tggcgcatcg gcgccgagag cgacctgggg
     1201 cgccagcagc agaatcggca ggctgaggat cgtggaggat ccgggcggca gcggcagggt
     1261 gttgagcgcg ccgaacacga acagcatcgc gccaaaggcg cgactgtcga aggcgtccag
     1321 catttcgccc acggtcaggg atggg
//
LOCUS       DAOMEO010001029         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9928_length_1345_cov_2.425581, whole genome shotgun sequence.
ACCESSION   DAOMEO010001029 DAOMEO010000000
VERSION     DAOMEO010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_1345_cov_2.425581"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1345)
                     /locus_tag="PLH31_18020"
                     /pseudo
     CDS             complement(51..1345)
                     /locus_tag="PLH31_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
BASE COUNT          212 a          408 c          445 g          280 t
ORIGIN      
        1 ggttcaattg ccggaagtga ttctggccgt tgacgcccag gtgcgcttta gctgatgtcg
       61 aaataggcct cgccgaacgc ggccgtgtcc cggtcgatcc gctgctgggc ggtcagccag
      121 atggtgtcgc tatagcccgg cacctcgatg tcgtagctga tgccgtcgat cttatagcgc
      181 tgggtaatcc cgtgctgctg cttctggtag aacaggccgc cgatcaggcg gaagcgcttg
      241 tcgtccgggg tggcgatccg gatctcgtgg ctttcctttt tgtagtcgtc cttgccggtg
      301 atgtactgcg acgggttggc caggccgccc gtgtcgttgg tccagtagaa gccgtagccg
      361 acctgatcgt agaaatagga atagtccgaa tagtcctgac gggtgtcgac ggtgcgcttg
      421 aggtacgagc cggcataggt gacgtcgaga ttggcgatct gcccctcgat ggtcatggcc
      481 gcctgcaccc acttgtcgtc cgaggcttcc ggatagaagt gagtcacctc caggtcaccg
      541 accttgggat cataggaata gatgccgctg gtcttggtgc gctggcccat gatcgtgggc
      601 gtgatcaccc agttctcgcc cagctcgacc cgcagggcgg cgcgaccgcc gcgggtgctg
      661 gagccgttat agttttcgct ggcggtggtg gcgttgctga tcgtcaggtc atcgctggga
      721 tcggccgtgc cgcgcccgta ggtgcgcgtg cccacgacgt tgtcgatata gccggcggtt
      781 tcctcgttcc agccgaccag gcgcaccgcg atggtgtccg acagcgggat gttgacgaag
      841 gcttcttcga catgacccat gtcgtcgcca tcaacctggt tgacctcgac atcaacgccc
      901 gccgaaaagc cggcggtgct gggcttgttg gtgatgatcc gcagggtgcc ggcctgtgag
      961 ctggcgccat acagcgtgcc ctgcggtccg gccagggcct cgacccgggc cacgtcatag
     1021 acgtggatgt ccagcgcgcc ctggatggtg ctgatcggct gttcgtccag atagacgccg
     1081 acgctgggca gcgagccgga gtggttgccg tcgccgccgc tggcgacgcc gcgcatatag
     1141 acctggccaa agccggggcc cgccgtcgtg aacgaaacgc tgggcagata tttcacatag
     1201 tcgacgaagt tggagatctg cagctgttcc agcttggcct cgcccagcgc ctggatagac
     1261 accggcacat cctgcagatt ctcggaccgc ttctgggcgg tgaccaccac ctcttccagc
     1321 gacaccacct gccgggcctg cgcgg
//
LOCUS       DAOMEO010001030         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9932_length_1345_cov_1.728682, whole genome shotgun sequence.
ACCESSION   DAOMEO010001030 DAOMEO010000000
VERSION     DAOMEO010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9932_length_1345_cov_1.728682"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLH31_18025"
     CDS             <1..297
                     /locus_tag="PLH31_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91125.1"
                     /translation="EAIVELVQQTAQMPDISNLATKDDIRDLATKTEVAALATKAELQ
                     ATNAEIRAATLELRGEMSSLEARLSEKMRVQGLALIGAIATLLALSTALIKLAP"
     gene            complement(294..725)
                     /locus_tag="PLH31_18030"
     CDS             complement(294..725)
                     /locus_tag="PLH31_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQR91126.1"
                     /translation="MSLHGTYDPDNIFAKIIRGEMPAVKVFEDDQVLAFMDVFPQARG
                     HMLVISKTSKARNLLEAEAKTLGRMIGATQKAAAAVTAALKPDGVIVTQFNGAPAGQT
                     VFHLHFHVIPRYEGEAMGRHGEGMADVEELKALAEKIAAVL"
     gene            complement(796..>1345)
                     /locus_tag="PLH31_18035"
     CDS             complement(796..>1345)
                     /locus_tag="PLH31_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HQR91127.1"
                     /translation="REFFFSFVRTQLFGGKLKQAQVNGLTAILDYWDAHLAAKDDRWL
                     AYALGTAHHETDKTFGPIREYGRGKGKAYGAPTAPHGHVYYGRGLVQLTWKDNYQKMS
                     SKLSVDLVADPDLALSLVNAVPVMFLGMEQGLFTGVGFGRYFNATRDDWVNARRIING
                     TDKANLIADHARMFYAAISHTV"
BASE COUNT          260 a          422 c          419 g          244 t
ORIGIN      
        1 gaggccatcg tggaactggt tcagcagacg gcgcaaatgc ccgacatcag taatctcgcc
       61 accaaggacg acatccgcga tctggcaacc aagactgaag tggcggcgct cgccaccaag
      121 gccgagttgc aggccacgaa cgcggaaata cgcgcagcga ccctcgaact cagaggcgaa
      181 atgtcctccc ttgaggcgcg cctgagcgag aagatgcgcg ttcagggctt ggcgttgatc
      241 ggtgcgatag ccacgctttt ggccctgtcc accgcactga tcaaactggc tccctaaagc
      301 acagccgcga tcttctcagc caaggccttc agttcctcga catcggccat gccctcgccg
      361 tgccggccca tcgcttcgcc ctcgtagcgg gggatgacgt ggaaatgcag gtggaacacc
      421 gtctggccgg ccggggcgcc attgaactgg gtgacgatca ccccgtcggg cttcagggcg
      481 gcagtgacgg cggcggcggc cttctgggtg gcgccgatca tccggccaag cgtcttggcc
      541 tcggcctcca gcaggttgcg ggcctttgag gtcttggaga tcaccagcat gtggccgcgc
      601 gcctgcggga acacgtccat gaaggccagg acctgatcgt cctcgaacac cttcacggcc
      661 ggcatctcgc cccggatgat cttggcgaag atgttgtcgg gatcgtaggt tccgtgaagg
      721 ctcatgacca tctcctgttc aggccgacgg tcgtatcaaa ccacgggggc ggcgcaagcc
      781 atgggtcaac acggatcaga ccgtgtggct gatagcggcg tagaacatgc gcgcatggtc
      841 ggcgatcagg ttggccttgt cggtcccgtt gatgatccgg cgcgcgttga cccagtcatc
      901 ccgggtcgca ttgaagtagc gcccgaagcc gaccccggtg aacagaccct gctccatgcc
      961 caggaacatc acgggcacgg cgttgaccag cgacagggcc agatcaggat cagccaccag
     1021 gtcgacgctc agcttgctgg acatcttctg atagttgtcc ttccaggtca gctgcaccag
     1081 gccacggccg taatacacat ggccatgagg agcggttgga gcgccatagg ccttgccctt
     1141 gccccgacca tattcgcgga tcggcccaaa ggtcttgtcg gtctcatggt gagcggtgcc
     1201 aagggcatag gccagccagc ggtcgtcctt tgccgccaga tgcgcgtccc aatagtcgag
     1261 aattgccgtc aggccgttga cctgagcttg cttcagcttg ccgccgaaca gttgcgtcct
     1321 gacgaaggaa aagaagaact cccgg
//
LOCUS       DAOMEO010001031         1344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9948_length_1344_cov_1.895268, whole genome shotgun sequence.
ACCESSION   DAOMEO010001031 DAOMEO010000000
VERSION     DAOMEO010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9948_length_1344_cov_1.895268"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLH31_18040"
     CDS             complement(<1..348)
                     /locus_tag="PLH31_18040"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQR91128.1"
                     /translation="MSSAPTTPLRQRMIEDMSIRQFGERTQRGYVAVVADFARFLGRS
                     PDKADPEDLRRYQLHLAAQGVSPGKMNAAVSALRFFFNLTLGRQGYGERLARARKEDR
                     LPEVLSPEEVALLL"
     gene            complement(706..>1344)
                     /locus_tag="PLH31_18045"
     CDS             complement(706..>1344)
                     /locus_tag="PLH31_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQR91129.1"
                     /translation="PAGEDPALYPVDAPFDPVKAGAALGVFTLVVTVAALGLLPIAAA
                     AFAGAVLLVVTGVISVEDAYKGLRPEILLLIAGMVVVGLSLELTGLAALTTDALVGWL
                     RGLGPLFALALIYGATLFLTEILSNAAVAVLLTPMAVALAESLGVNPRPFLVAVMMAA
                     SAAFATPFGYQTNVLVYQMGGYRYLDFVRIGAPLNLITWAAGMAAIPIFFPF"
BASE COUNT          253 a          424 c          464 g          203 t
ORIGIN      
        1 cagcagtagc gccacctctt cagggctgag cacttcgggc aggcggtctt ccttgcgcgc
       61 cctggccaag cgctcgccat aaccctgccg acccagggtg aggttgaaga agaaccgtag
      121 ggccgagacg gcagcgttca tcttgccggg cgaaacgcct tgggcggcca gatgcagttg
      181 gtatcgtcgc agatcctcgg gatcggcctt gtccggcgag cggccgagga accgagcgaa
      241 gtcggcgacc acggcgacat agccgcgctg ggtccgctcg ccgaactggc ggatggacat
      301 gtcttcgatc atccgctgac gcagcggggt ggtgggtgca gagctcatcg gggatctcct
      361 gtctgcaggg ttgacccctg atcttcagac aggacgggca aggcgccact tcaggttcgt
      421 tcacggcgcc agcgccggtc acccatgccg cgcgagcggc ttagtccgct ggcgctcggg
      481 cggcttgaaa ccttatcggc aggtatcgaa gagttcgccc ctgattctca ttccaaatga
      541 gccgcggggc aagtttctca ctttgagtga gctcgcatga gggcgagtct cactctgagc
      601 gagctgggcg tctcaactgg tttgagctgg ggagtggaaa agccgaggct tatcagcgac
      661 ggttctcagc ttgagtgagc cggccgaatc gctgcgcggc cgggttcaga aggggaagaa
      721 gatcgggatg gccgccatgc cggcggccca ggtgatcagg ttcagcggcg cgccgatccg
      781 cacgaagtcc agatagcgat agccgcccat ctgatagacc agaacgttgg tctgatagcc
      841 gaacggcgtg gcgaaggcgg cgctggcggc catcatcacc gccaccagga agggtcgcgg
      901 attgacgccc aggctctcgg ccagggccac agccatcggc gtcagcaaca ccgcgacggc
      961 ggcgttggac aggatctcgg tcaggaacag ggtggcgcca tagatcagcg ccagggcgaa
     1021 cagcggtccc agaccgcgca gccatccgac gagggcgtcc gtggtcaggg ccgccagacc
     1081 ggtcagttcc agggaaaggc caaccaccac catgccggcg atcagcagca ggatttcggg
     1141 gcgcaggccc ttataggcgt cctcgacgct gatcacgccg gtcaccacca gcaacaccgc
     1201 cccggcgaag gccgccgccg cgatcggcag caggcccagg gccgcgaccg tgaccacgag
     1261 ggtgaaaacc cccagggccg cgccggcctt caccggatcg aaaggcgcgt cgacgggata
     1321 gagcgcggga tcctcgccgg cggg
//
LOCUS       DAOMEO010001032         1344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9950_length_1344_cov_1.757952, whole genome shotgun sequence.
ACCESSION   DAOMEO010001032 DAOMEO010000000
VERSION     DAOMEO010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9950_length_1344_cov_1.757952"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>678
                     /locus_tag="PLH31_18050"
     CDS             <1..>678
                     /locus_tag="PLH31_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HQR91130.1"
                     /translation="EDDPETLATLKRMGFSSPERVAATIRSWHHGHIAATRTERGREL
                     FTRLAPRLLDAANATGAPDAAFNRFADFFASLSSGVQIQSLFLAQPRLFELIVEVMAF
                     APRLAATLARRPTALDALLDPAFFGPMEMPEANWDPEDFEGAMNAARRLFRDQSFRIG
                     VRVMSGTAGARDVGAAFAGLGDLIVGGLAPAALAETVRLGGDFPGEVAVVALGKAGSR
                     EMTAKSDL"
     assembly_gap    679..688
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <689..>1344
                     /locus_tag="PLH31_18055"
     CDS             <689..>1344
                     /locus_tag="PLH31_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HQR91131.1"
                     /translation="SSIKAWGAESFYARFTQRLTSALSAPTGEGTLYEVDLKLRPSGT
                     KGPVAVSFAAFEDYYDREAETWELLALTRARVMWASSPAFKVRAEAAIEAALRRPRDP
                     VKTAVDVLEMRELMEQERPGKGVWDLKLTPGGLVDIEFVAQFLQLKYAADGGPLAQNT
                     GEALAALRAAGLADDAALASLETAWRLEQDLSQLIKVALEDGGDPEAEPKAFKTLLAR
                     "
BASE COUNT          183 a          450 c          483 g          218 t
ORIGIN      
        1 cgaggatgac cccgagaccc tggccacgct caagcgaatg ggcttttcca gccccgagcg
       61 cgtcgccgcc accatccgca gctggcacca cggccatatc gccgccaccc gcacagagcg
      121 gggccgcgag ctgttcacgc gcctggcgcc gcgcctgctg gacgccgcca acgctacggg
      181 cgcgccggac gccgccttca accgcttcgc cgacttcttc gcctccttga gttccggcgt
      241 gcagatccag tcgctgttcc tggcccagcc gcgtctgttc gagctgatcg tcgaggtcat
      301 ggcctttgcg ccgcgcctcg ccgccaccct ggcccgccgg cctacggctc tggacgccct
      361 gctggatccg gccttcttcg ggccgatgga aatgcccgag gccaattggg atccagagga
      421 tttcgagggc gcgatgaacg ccgcgcgccg cctgttccgc gaccagagct ttcgcatcgg
      481 cgtgcgggtg atgagcggga ccgccggggc ccgcgacgtc ggcgcggcct ttgccgggct
      541 gggcgacctg atcgtcggcg gcctggcgcc ggcggccctg gccgagaccg tccggctggg
      601 cggcgacttc ccgggcgagg ttgcggtggt ggccctgggc aaggccggct cgcgcgagat
      661 gaccgccaag tctgacctnn nnnnnnnngt cgtcgatcaa ggcctggggc gccgaaagct
      721 tctatgcccg cttcacccag cgtctgacct cggccctgtc ggcgccgacc ggggagggca
      781 cactctatga agtggacctg aagctgcggc cttcaggcac caaggggccg gtcgcggtca
      841 gctttgccgc ctttgaagat tattacgacc gcgaggccga gacctgggag ctgctggccc
      901 tgacccgcgc ccgggtgatg tgggccagct cgccggcctt caaggttagg gccgaggcgg
      961 ccatcgaggc ggccctgcgc cgtccgcgcg atccggtgaa gactgctgtc gacgtgctgg
     1021 agatgcgcga actgatggag caggagcggc cgggcaaggg cgtctgggac ctgaagctga
     1081 cgcccggcgg gctggttgat atcgagttcg tcgcccagtt cctgcagctc aagtatgcgg
     1141 ccgatggtgg ccctctggcg cagaataccg gcgaggccct ggcggcgctg agggcggcgg
     1201 gtctggctga tgatgcggct ttggcgtcgc tggagacggc atggcggctt gagcaggacc
     1261 tgtcgcagct gatcaaggtg gcgctggagg atggcggcga tcccgaggcc gagccgaagg
     1321 cgttcaagac gttgctggcc cgcg
//
LOCUS       DAOMEO010001033         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9959_length_1343_cov_1.975155, whole genome shotgun sequence.
ACCESSION   DAOMEO010001033 DAOMEO010000000
VERSION     DAOMEO010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9959_length_1343_cov_1.975155"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLH31_18060"
     CDS             <1..210
                     /locus_tag="PLH31_18060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HQR91132.1"
                     /translation="EVTSATALTAAQLAGVQAALASALGKTPELSTRVDASLLGGVKV
                     RVGSRLFDASLRSKLDSLKFALKRA"
     assembly_gap    257..266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            276..>1343
                     /gene="atpA"
                     /locus_tag="PLH31_18065"
     CDS             276..>1343
                     /gene="atpA"
                     /locus_tag="PLH31_18065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQR91133.1"
                     /translation="MDIRAAEISAILKSQIANFGEEAAVSDVGQVLSVGDGIARIYGL
                     DNVQAGEMVEFPKAGVKGMALNLERDNVGCVIFGQDQAIKEGDEVRRLGEIVDVPVGR
                     GLLGRVVNPLGEPIDGKGPIVSTERRRVDVKAPGIIPRKSVHEPVQTGLKSIDTLIPV
                     GRGQRELIIGDRQTGKTAVAIDTILNQKAANAGKDESAKLYCVYVAIGQKRSTVAQIV
                     KTLEEHGALEYTIVVVASASEPAPLQYLAPFSGCAMGEWFRDNGLHGLIIYDDLSKQA
                     VAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNEDNGSGSLTALPIIETQAND
                     VSAYIPTNVISITDGQIFLETD"
BASE COUNT          245 a          469 c          394 g          225 t
ORIGIN      
        1 gaggtcacct cggcgaccgc actgaccgcc gcccagctgg ccggcgtgca ggccgccctg
       61 gcttcggccc tgggcaagac ccctgaactc tcgacccgcg tcgacgcttc ccttctgggc
      121 ggcgtcaagg tgcgcgtcgg ctcccgcctt ttcgatgctt cgctccgttc gaagctcgat
      181 tccctgaaat tcgccctgaa gcgcgcctaa acctttataa gcgcgtcaaa agataaaaat
      241 cgcagagagc ccaagtnnnn nnnnnnagta cagccatgga catccgcgcc gccgagattt
      301 cggccatcct caagtcgcag atcgccaatt tcggcgaaga agccgctgtt tccgacgtcg
      361 gccaggtgct gtcggtcggt gacggcatcg cccgcatcta cggtctggac aacgtccagg
      421 ccggcgaaat ggtcgagttc cccaaggccg gcgtgaaggg catggccctg aacctcgagc
      481 gcgacaacgt gggttgcgtg atcttcggtc aggaccaggc catcaaggaa ggcgatgaag
      541 tccgtcgtct cggcgagatc gtcgacgttc cggtcggccg tggcctgctg ggccgcgtcg
      601 tcaacccgct gggcgagccg atcgacggca agggcccgat cgtctcgacc gagcgtcgcc
      661 gcgttgacgt caaggctccg ggcatcatcc cccgcaagtc ggtgcacgag cccgtgcaga
      721 ccggcctgaa gtcgatcgac accctgatcc ccgtcggccg cggccagcgc gagctgatca
      781 tcggtgaccg tcagaccggc aagaccgccg tcgccatcga caccatcctg aaccagaagg
      841 ccgccaacgc cggcaaggac gagagcgcca agctctattg cgtctatgtc gccatcggcc
      901 agaagcgttc gaccgtcgcc cagatcgtca agacgctcga agagcatggc gccctggaat
      961 acaccatcgt cgtcgtcgcc tcggcctccg agccggcccc gctgcaatac ctggcgccgt
     1021 tctcgggctg cgccatgggc gagtggttcc gcgacaacgg cctgcacggc ctgatcatct
     1081 atgatgacct gtccaagcaa gccgtcgcct atcgccagat gtccctgctg ctgcgtcgcc
     1141 cgccgggccg cgaagcctat ccgggcgacg tcttctacct gcactcccgc ctgctggaac
     1201 gcgccgccaa gctgaatgaa gacaacggtt cgggctcgct gacggccctg ccgatcatcg
     1261 aaacccaggc caacgacgtt tcggcctata tcccgaccaa cgtgatctcg atcaccgacg
     1321 gccagatctt cctggaaacc gac
//
LOCUS       DAOMEO010001034         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9984_length_1341_cov_1.812597, whole genome shotgun sequence.
ACCESSION   DAOMEO010001034 DAOMEO010000000
VERSION     DAOMEO010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9984_length_1341_cov_1.812597"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..596)
                     /locus_tag="PLH31_18070"
     CDS             complement(12..596)
                     /locus_tag="PLH31_18070"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQR91134.1"
                     /translation="MTLDPDILADLAVEITPKAYVRVTPMAHAATPLGAGFGVTRFAS
                     PTKAFKVIYLGQDLTTSVAETLVRDRFQGRATRKLLDVEAATWGATEVTASAPLTLID
                     LRTTGLVRLGVSTEAARGKAQGQGRKLRQAVYDQTDAHGLVYSSRLTGRTCICVYDRA
                     LPGALGASPVIEVTRLAGFLDALQALNVTLIATP"
     gene            complement(593..>850)
                     /locus_tag="PLH31_18075"
     CDS             complement(593..>850)
                     /locus_tag="PLH31_18075"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HQR91135.1"
                     /translation="WQKQGAVIGLLVGVRKHAFPTEQFVDGRPVSGLGPVVEAIGEPR
                     TAWLWLREPNPELGGAAPLARLKAGATDKVVEVARSNFAGA"
     assembly_gap    851..860
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<861..1216)
                     /locus_tag="PLH31_18080"
     CDS             complement(<861..1216)
                     /locus_tag="PLH31_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91136.1"
                     /translation="MAADLIGMFAQRNPESSKGELSEVVKLVAEFSATATKLSPSARK
                     RLIARKAQFKKFIAEIAAEPEAAPQVLKLVAKAPAEVSQGAGLGPVLSAEEGKARIAS
                     YATPAKVEDWAGPLAGP"
BASE COUNT          194 a          443 c          461 g          233 t
ORIGIN      
        1 tgggggcgct gttagggcgt agcgatcagg gtgacgttga gggcctgcag ggcgtccagg
       61 aagccggcca gccgagtgac ttcgatgacc ggcgatgcgc ctaacgcgcc tggcagggcg
      121 cggtcgtaga cgcagatgca ggttcggccg gtcaggcgcg agctgtagac caggccgtgg
      181 gcgtcggtct ggtcgtagac cgcttgcctg agcttgcggc cctgcccttg cgcctttccg
      241 cgtgcggctt ctgtcgagac acccagccgc accaagcccg tcgtgcgcaa atcaatcaga
      301 gtcagcggtg cgctggcggt gacttcggtc gcgccccagg tcgcggcctc gacatccagg
      361 agcttgcggg tcgccctgcc ctggaagcgg tcgcggacca gggtttccgc cacgctcgtg
      421 gtcaggtcct ggcccaggta gatcaccttg aacgccttgg ttgggctggc gaagcgcgtc
      481 acgccaaacc ccgcccccag gggcgtcgcc gcgtgggcca tcggcgtcac gcgcacatag
      541 gccttcggcg tgatctcgac ggcgaggtcg gccaggatgt cgggatcgag cgtcacgcgc
      601 cggcgaagtt cgaccgggcg acctcaacga ccttgtcggt cgccccggcc ttcaggcgcg
      661 ccaacggggc ggcgccgccg agctcaggat tgggttcgcg cagccatagc caggcggtgc
      721 gcggctcgcc gatcgcctcg acgaccgggc cgaggcccga gacgggccgg ccgtcgacga
      781 attgttcggt cgggaaggcg tgtttacgca cgcccacgag caggccgatc accgcgccct
      841 gcttttgcca nnnnnnnnnn ggacccgcaa gcgggcccgc ccaatcctcg accttggccg
      901 gcgtcgcata gctcgcgatc cgcgccttgc cctcctcggc cgacagcacc gggcctagac
      961 ccgcaccttg gctcacttcg gcgggcgcct tggcgaccag cttgaggact tgcggcgcgg
     1021 cttccggttc ggcagcgatt tcggcgatga acttcttgaa ctgggccttg cgcgcgatca
     1081 gtcgtttgcg ggcggacggc gagagtttcg tcgccgtggc gctgaactcg gcgaccaact
     1141 tgacgacctc gctaagctcg cctttgctgg actccggatt gcgctgggca aacatgccta
     1201 tcaggtctgc ggccacaggg ctggccatct tgccagggcc tcgagacgtc gagcggcgag
     1261 cgtgggcggc ggtcatggga cgatcctctt cgatcacgac gttagagata atcctaaacg
     1321 tcccatttgt ccagtttgtg c
//
LOCUS       DAOMEO010001035         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9985_length_1341_cov_1.772162, whole genome shotgun sequence.
ACCESSION   DAOMEO010001035 DAOMEO010000000
VERSION     DAOMEO010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9985_length_1341_cov_1.772162"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLH31_18085"
     CDS             complement(<1..790)
                     /locus_tag="PLH31_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR91137.1"
                     /translation="MNTPLSRLAPNAQGAIWLLLSALTFVTMTTLVKHLSGDYPSHLQ
                     NFYRQTGSLIVAIPFMLRNPRQVLWVPRSTMPALFLRSLLATIGMILLLYTYEAMPMA
                     EANALSFTRPLWVVLLAALFLRETIGPSRIAAVVIGFVGVVIIMRPWGAGVTVGWPHL
                     AALISALFLAGTITGVKSLTRDLSASSILVWSSIMGELLSLPFALADWRWPNPGDLIP
                     LFLIGLLSAANQVLFIKGMAVGEAAVLAPIDYARLVLSVIAGVVV"
     gene            complement(787..>1341)
                     /locus_tag="PLH31_18090"
     CDS             complement(787..>1341)
                     /locus_tag="PLH31_18090"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQR91138.1"
                     /translation="DLRVLRRSRLIMARVAPLPLDGLSDPLRASIDRGKATRMLSNTL
                     PVQIWAHRPAAANAWLGVMAEIHERGVLPDRLRELMRLKIASITTCPVCQIARKSDDV
                     SEADIVACSDWSDPRFSPPEQAALRFAELFAGDYFSMGDEVYDHLREHFSEAQIVELH
                     LFSGLMLAGGRMTYVLKGYEDNPA"
BASE COUNT          265 a          403 c          479 g          194 t
ORIGIN      
        1 acacgaccac gcctgcgatc accgacaaga ccagccgcgc atagtcgatg ggcgccagga
       61 cggcggcttc gccgaccgcc atccccttga tgaacagcac ttggttggcg gcgctgagta
      121 ggccgatcag gaagagtgga atcagatcgc cagggttggg ccagcgccag tcagcgaggg
      181 cgaagggtag ggacagaagt tcgcccatga tgctggacca gaccaggatc gagctcgcgg
      241 acaggtcgcg ggtcagggac ttcacgcccg tgatcgtgcc ggccagaaac agagccgaga
      301 tcagcgcggc caggtgcggc cagccgactg tgacccccgc gccccatggc cgcatgatga
      361 tgacgacacc gacaaagccg atcaccaccg ccgcgatccg gctggggccg atcgtctcgc
      421 gcaggaacag cgccgccaac agcacgaccc aaagcggccg ggtgaacgac agggcgttgg
      481 cctcggccat gggcatggct tcataggtgt agagcagcag gatcatgccg atggtcgcca
      541 gaagcgagcg caggaacagg gcgggcatcg tcgagcgcgg cacccacaga acctgtctgg
      601 gattgcggag catgaagggt atggcgacga tcagcgaacc ggtctggcga tagaagttct
      661 ggaggtgcga ggggtagtcg ccgctgaggt gcttcaccag ggtggtcatg gtgacgaacg
      721 tcaaggccga gagcagcagc cagatcgcgc cctgggcgtt cggcgccagg cgcgagaggg
      781 gcgtgttcac gcggggttgt cctcatagcc cttcagcaca taggtcatgc ggccgccggc
      841 cagcatcagg ccgctgaaca ggtggagttc gacgatctgc gcttcgctga aatgctcgcg
      901 cagatgatcg tagacctcgt cgcccatgga gaaatagtcg ccggcgaaca gttcggcgaa
      961 ccgcagggcg gcctgttcgg gcgggctgaa gcgcgggtcg gaccagtcgc tgcaggcgac
     1021 gatgtcggcc tcgctgacgt cgtcggattt gcgggcgatc tggcacaccg ggcaggtcgt
     1081 gatcgaggcg atcttgagcc gcatcagttc gcgcagccgg tcgggcagaa cgccacgctc
     1141 gtgaatctcg gccatcacgc cgagccaggc gttggctgcg gccggccgat gcgcccagat
     1201 ctggaccggc agggtgttgg atagcatccg cgtcgccttg ccccgatcga tcgaggcgcg
     1261 aagcggatcg gacagaccat cgaggggcag gggggcgacg cgggccatga tcagccgcga
     1321 tcttcgcaga actcgaagat c
//
LOCUS       DAOMEO010001036         1340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_9992_length_1340_cov_2.128405, whole genome shotgun sequence.
ACCESSION   DAOMEO010001036 DAOMEO010000000
VERSION     DAOMEO010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9992_length_1340_cov_2.128405"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLH31_18095"
     CDS             complement(<1..190)
                     /locus_tag="PLH31_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91139.1"
                     /translation="MKPFAASLPASARCSLFGAALAVFSLVSAQPAGAQLVSAAAQTF
                     DIPSQEASSALVQFCLQAD"
     gene            complement(263..1234)
                     /locus_tag="PLH31_18100"
     CDS             complement(263..1234)
                     /locus_tag="PLH31_18100"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQR91140.1"
                     /translation="MTILFAKRPRTAEDWVARMGNPVVGARMQAAFLAWLESDPEHLR
                     RYELAKAEQAALEDLRGTFAMDLARLRRSERHVASPRRFVLAGGLAVAALAVAAVVLI
                     PSPEARLYESAPGQIMDVALDDGSRVTLDADSAIRVSLTGDVRRVILERGAAYFDVAH
                     DATHPFQVAVGDRRVIVTGTRFVTALTGDGAHISLLQGRVVVGTRDAAEKAAVQGALA
                     LAPGERAVFHPGQPAIEKARVDVEMTTAWRKRRLVFQDASLSDVVVAAARYSERPLVV
                     ADPELARVKVTAVLPLEGEGTLVARMTALLPIETRQTADGRVLIRAQ"
BASE COUNT          274 a          455 c          419 g          192 t
ORIGIN      
        1 aatcggcttg cagacagaac tgaaccaagg ccgaggaggc ttcctgagac gggatgtcaa
       61 aggtttgggc ggccgcgctg acaagctgcg ccccagcggg ttgcgcggaa accagcgaaa
      121 agaccgcgag ggctgcgccg aacaggctgc atcgcgcgct agcgggcagc gatgcagcga
      181 acggtttcaa accttgcggc ccccagcgag acaatccaca aacccgttag cgcgctggcg
      241 tgacagctct gcaatcggca gcctactggg cccgaatcag cacccggcca tcagcggtct
      301 gccgcgtctc gatagggagc aaggccgtca tgcgggcgac caaagtcccc tcgccttcca
      361 gcggcaacac ggcggtcacc ttgacacgcg ccaactcagg atcagcgacg accagcggcc
      421 gctctgaata gcgcgcggca gcgacgacca cgtcagacag cgatgcgtcc tggaaaacca
      481 gacgacgctt acgccaggct gtcgtcatct ccacgtcgac gcgggccttc tcgattgcag
      541 gctggccggg gtgaaagacg gcccgctcgc caggcgccag agccagagcg ccctgcaccg
      601 cagccttttc ggccgcatct cgcgtcccga cgacgacgcg cccctggagc agagaaatgt
      661 gggcgccgtc cccggtcagg gcagtcacga accgggtgcc tgtaacgatc acgcgccggt
      721 cgccgacagc cacctggaag ggatgggtgg cgtcatgagc cacgtcgaaa taggccgcgc
      781 cgcgttcaag gatcacccgc cgcacatcgc ccgtcagaga aacgcgaatg gctgagtccg
      841 cgtcgagggt gacgcgagag ccgtcgtcca gcgcgacgtc catgatttgg ccaggtgcgc
      901 tttcgtagag acgcgcttcc ggcgacggga tcagcacaac cgcagcgaca gcgagggcgg
      961 cgaccgccag cccgccagcg agcacgaaac gacggggcga ggccacatgg cgctcacttc
     1021 ggcgcaatcg ggccagatcc atcgcaaagg tcccgcgcag gtcctccagc gcggcctgtt
     1081 cggctttggc cagctcgtat cgcctcaaat gttccggatc ggattccagc caagcgagga
     1141 aggccgcctg catgcgcgca ccaaccactg gattacccat tcgggccacc cagtcctccg
     1201 ccgtccttgg ccgcttggcg aacagaatgg tcaagcggtc aagcatagac aacgagcgtc
     1261 cttttgtatc gagcggcccc gatcctcgcg gcggtcgctt catacttcac aacgatacag
     1321 gggctcgaca cctcaatcat
//
LOCUS       DAOMEO010001037         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10000_length_1339_cov_3.224299, whole genome shotgun sequence.
ACCESSION   DAOMEO010001037 DAOMEO010000000
VERSION     DAOMEO010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10000_length_1339_cov_3.224299"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..>1339)
                     /locus_tag="PLH31_18105"
     CDS             complement(360..>1339)
                     /locus_tag="PLH31_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR91141.1"
                     /translation="IAGVTLMGVYGLGLGLASALLLGAVLAPTDPVLASDVQVGPPRS
                     GEDGEARFALTSEAGLNDGFAFPFVHLAVALAATGGVLKPDLLTHWLAMDVLWRIAAG
                     IAVGWAVGKGLGWITFKAPRARLSGTGDGLVALGATLFAYATAEAAHGYGFLAVFVAA
                     LTLRAAERDHAFHEAMHDFAEQVERLLMMLVLVLLGGAIASGLLAPLTWKDAALGLAL
                     LFIVRPLAGWIGMIGAPHSRRERAFVSFFGIRGIGSFYYLAWGLNHGEFDGWARLWAI
                     TGFIVLCSILIHGVTATPLMKTIDTWRRGPGRPDHEEPVDAAVAEDRQV"
BASE COUNT          252 a          474 c          441 g          172 t
ORIGIN      
        1 gacgcgctct ccagtgttcg acataaggcg cggcgaaggt ccaggcgagg gtcgccgcca
       61 gcaacaggac gatcggaatc gccagcttca gattcaagtc cgcctcccca ggcttttttt
      121 cgaggttagc gctggtcgcg gcgccgctca attcctcaaa cgtaaggcga gggcccgacc
      181 ggagaaagta acgcatctcc ggccggggag ggtcgtcgac gggtggggcc catcgacgct
      241 gatcggtttg cgacgaggac aagaggcttg caatggcgcc gcgactatgt cgcagccgca
      301 aacccaaggt tcccggcgga tcgcgccgtt tggtcgcgcc gggcggcgat cctgcgtgct
      361 taaacctgcc tgtcctcagc aacggccgcg tcaactggct cttcgtggtc cggacggcct
      421 ggcccgcgcc gccaggtatc gatcgttttc atcagcggcg tcgccgtcac gccgtgaatg
      481 aggatggagc acaggacgat aaaccccgtg atcgcccata ggcgcgccca gccgtcaaac
      541 tcgccgtgat tgaggcccca ggccagatag tagaaggagc caatgccgcg aatgccgaag
      601 aacgacacaa aggcccgctc gcggcgcgag tggggcgcgc cgatcatccc gatccagccg
      661 gccagcgggc ggacgatgaa cagcagcgcc aggcccaggg ccgcgtcttt ccacgtcagc
      721 ggggccagca ggccgctggc gatggcgccg cctagcagga ccagcaccag catcatcagc
      781 aggcgctcca cctgctcggc gaaatcatgc atcgcctcgt ggaacgcatg atcgcgctcg
      841 gccgcccgca gggtcagcgc cgcgacgaac acggccagga agccatagcc atgggccgcc
      901 tcggcggtgg cgtaggcaaa cagcgttgcg cccagcgcca ccagaccatc gcccgtgcct
      961 gacagccgcg cgcgcggcgc cttgaaggtg atccagccca ggcccttgcc caccgcccag
     1021 cccaccgcga tgccagccgc gatccgccac aggacgtcca tcgccagcca gtgggtcagg
     1081 agatcgggct tcagaacccc gccggtcgcc gccagggcga cggccagatg cacgaaggga
     1141 aaggcaaagc cgtcattgag cccggcctcg gaggtcaggg cgaaccgcgc ctcgccgtcc
     1201 tcccccgaac gcggcggccc gacctggacg tcggaggcca gcaccggatc ggtcggggcc
     1261 agcacggcgc ccagcagcag ggccgaggcc aggcccaggc ccaggccata gacgcccatc
     1321 aacgttaccc cggcgatcg
//
LOCUS       DAOMEO010001038         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10002_length_1339_cov_2.792835, whole genome shotgun sequence.
ACCESSION   DAOMEO010001038 DAOMEO010000000
VERSION     DAOMEO010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10002_length_1339_cov_2.792835"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..568
                     /locus_tag="PLH31_18110"
     tRNA            492..568
                     /locus_tag="PLH31_18110"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:526..528,aa:His,seq:gtg)
     gene            complement(775..>1339)
                     /locus_tag="PLH31_18115"
     CDS             complement(775..>1339)
                     /locus_tag="PLH31_18115"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR91142.1"
                     /translation="RFNFPNKFLVYQHDTPDKYLFKKDKRAYSHGCMRVLNPFTYGEK
                     LLSLALPEGHYTQAKLESMVGNSEININFPKPLWVHLTYQTAFVDENGKLQFRDDIYG
                     TDAKMIGILKGSERKVADLAVERPANQSSKPVRMPVGMYGGDRSYSSGPNFFEMLFGG
                     GRTPEVEPPTRRGLGPRYNREGRIVTR"
BASE COUNT          224 a          418 c          433 g          264 t
ORIGIN      
        1 atgcgcagcc agttcgacac cctgaccgcc caggccaagg aattgagcga gcacgcccag
       61 aaggtcgcga ccgagaccgc cgagccgatc aaggaaaagt tcgccagttt cggcaaagct
      121 gcctgatggc cgcctgacag ccgcgagaag gccgccaagg ccttcggcgg cgaccagcgc
      181 cacggccctg atggccgtgg cccacggacc gggctaaatg ccccggctaa atgaccaagg
      241 cgcgcggttc agccgcgcga tgcagtcaca ttacctagcc cgggcgtcgc gcctgggctt
      301 ttttgttccg ctgcgtcgcc gtcgcgggcc tggccggcag ggtgcccgtc ctcaagcgcc
      361 ctgtcgggaa gcccctgatc cgcaagttcc ctgtcccaaa ggcttattca aattggccgg
      421 ccaaaatgct tgccaaagag gggggcggct tctaggttcc ggctcgctcg gccggatggc
      481 cgggaacccg tgcagctgta gctcagttgg ttagagcgct ggtctgtgga tccagaggtc
      541 ggtggttcga gcccacccag ctgtaccaaa ctccaccctt catatttccg ttctgacggt
      601 atcgcggtcg ctgccggctt cgtcgcgact ggcgcggtcg ttgatgactt gatcgtggca
      661 atcggcagcc cttggcggcc gaacggtcgg gcgcgcactt cccctagccg tccggacgca
      721 aaaacgccgg cgaatttctt cgccggcgtt tggtgtagtc ggaagttgtt gagcctagcg
      781 cgtaacgatc cggccctcgc gattatagcg cggcccaagg ccacggcggg tcggcggttc
      841 gacttccggg gtccgtccgc caccgaacag catttcaaag aagttcgggc cgctcgagta
      901 gctgcggtcg ccgccataca tcccgaccgg catccgcacc ggcttggacg actggttggc
      961 cggccgctcg acggccaggt cggcgacctt gcgctcgctg cccttgagga tgccgatcat
     1021 cttggcgtcg gtgccgtaga tgtcgtcgcg gaattgcagc ttgccgttct cgtcgacgaa
     1081 ggccgtctga taggtcaggt gaacccagag cggcttcggg aagttgatgt tgatctcgct
     1141 gttgccgacc atgctttcca gcttggcctg ggtgtagtgg ccttccggca aggccagcga
     1201 cagcagcttc tcgccgtagg tgaaggggtt cagaacccgc atgcagccgt ggctgtaggc
     1261 gcgcttgtcc ttcttgaaca aatatttgtc cggcgtatcg tgctggtaga ccaggaactt
     1321 gttcgggaag ttgaagcgg
//
LOCUS       DAOMEO010001039         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10004_length_1339_cov_2.556075, whole genome shotgun sequence.
ACCESSION   DAOMEO010001039 DAOMEO010000000
VERSION     DAOMEO010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10004_length_1339_cov_2.556075"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLH31_18120"
     CDS             <1..875
                     /locus_tag="PLH31_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR91143.1"
                     /translation="ELAINGWAMEARLYAENPTAGFLPSTGPLDRLRLPKEVRVDTGV
                     YEGGEVTPFYDPMIAKLIVHAPTRQAAAAKLAAAARQVQVWPVRTNAAFLARAAADGD
                     FVAGRVDTGFIERGIEALVPTAEPSAAIQAAAALALVPAQTERAWTDLPGFRANAPAQ
                     RQVAVSIAGSIHLAEAASSAKGCVASVGGRDILFLDGEAWPIGPGRVDAISGGGASDG
                     AILSPMPGRVIAVDVEEGQAVTRGQKLLTLEAMKMEHGMVAPFDGVVMMLSVKAGDQV
                     GEGAVLARIEARAD"
     gene            875..1324
                     /locus_tag="PLH31_18125"
     CDS             875..1324
                     /locus_tag="PLH31_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR91144.1"
                     /translation="MAGRHFEDWTVGDRLAHEIRRTVTETDNLLFSVMTHNPQPLHLD
                     AEAAKASEFGRILVNGTFTFALMIGLSVGDTTLGTLVANLGYDTLVMPAPVFIGDTMH
                     AETEVTALKDSRSRPTAGLVTFSHRLLNQRDEVVCSCLRTALVSRRP"
BASE COUNT          201 a          462 c          456 g          220 t
ORIGIN      
        1 acgagctcgc gatcaacggc tgggccatgg aggcgcgcct ctacgccgag aacccgaccg
       61 ccggcttcct gccgtccacc ggcccgctgg accggttgcg gttgcccaag gaggtgcgcg
      121 tcgacaccgg cgtctacgag ggtggtgagg tcaccccctt ctacgacccg atgatcgcca
      181 agctgatcgt ccatgcgccc acacgtcagg ccgccgccgc gaaactagcc gcggccgccc
      241 gccaggttca ggtctggccc gtccgcacca acgccgcctt cctggcccgc gcggccgccg
      301 acggcgattt cgtggccggc agggtggata ccggcttcat cgagcgcggc atcgaggccc
      361 tggtcccgac cgccgagccc tccgccgcca tccaggcggc cgccgccttg gcgctcgtcc
      421 cggcgcagac ggagcgcgct tggacggacc tgccgggctt tcgcgccaac gctccggcgc
      481 agcgccaggt cgccgtctct atcgccggtt ccatccacct ggccgaagct gcgtcgagcg
      541 ccaagggatg cgtcgcttcg gtcggcggac gcgacatcct gttcctcgac ggcgaggctt
      601 ggccgatcgg tccgggacgc gtggacgcca tcagcggcgg gggcgcttcc gacggcgcga
      661 tcctcagtcc catgccgggg agggtcatcg cggtcgatgt tgaagagggc caggcggtca
      721 cacgcggaca gaagctcctg acgctggagg ccatgaagat ggagcacggc atggtcgccc
      781 cgttcgacgg cgtggtcatg atgttatcgg tcaaggccgg cgaccaggta ggggagggcg
      841 cggttctcgc ccggatcgag gcgagggcgg actgatggct ggacgccact tcgaggattg
      901 gacggtcggt gatcggttgg cgcacgagat ccgtcgcacc gtgaccgaga ccgacaacct
      961 cctgttttcg gtcatgaccc acaatccgca accgctgcat ctggacgccg aggcggccaa
     1021 ggccagcgag ttcggtcgca tcctggtcaa cggcaccttc accttcgccc tgatgatcgg
     1081 cctttcggtg ggcgatacga cgctggggac tttggtcgct aacctcggat acgacacgct
     1141 ggtcatgccg gcgccggtct ttatcggcga caccatgcat gccgagacgg aggtgacggc
     1201 gctcaaggac agccgctccc ggccaaccgc cggtctggtc accttctcgc acaggctgtt
     1261 gaaccagcgc gacgaggtgg tctgttcgtg ccttcggacc gctctggtga gccgccggcc
     1321 atgaggttgc gatcgcttc
//
LOCUS       DAOMEO010001040         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10006_length_1339_cov_2.409657, whole genome shotgun sequence.
ACCESSION   DAOMEO010001040 DAOMEO010000000
VERSION     DAOMEO010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10006_length_1339_cov_2.409657"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLH31_18130"
     CDS             complement(<1..370)
                     /locus_tag="PLH31_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQR91145.1"
                     /translation="MTSPTIPHGPLRSFGRIKSRPVKPRQQALLDTLLPEIAVPEAPF
                     EPRDLMPEATEAWLEIGFGGGEHMAAQAGRHPHALIIGAEPFVNGVASAVRHIEERAL
                     RNVRIHEGDARDVIGWLPDAC"
     gene            complement(581..>1339)
                     /gene="metK"
                     /locus_tag="PLH31_18135"
     CDS             complement(581..>1339)
                     /gene="metK"
                     /locus_tag="PLH31_18135"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQR91146.1"
                     /translation="AVLMPMPIHLAHRLTERQSELRKDGRLPWLRPDAKSQVSIRYVD
                     GKPHSIDTIVLSTQHHPEVSHAVLTEAVIEEIIKPVVPKGMLTADTRYLVNPTGRFVV
                     GGPMGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAGL
                     ADRCAYAIGVAQPVSFHVDLHGTGKVDPAVLEKILPQLIGGATPRSIREHLQLNKPIY
                     ARTAAYGHFGRTPDNEGGFSWEKTDLVGELKGLA"
BASE COUNT          203 a          428 c          492 g          216 t
ORIGIN      
        1 ggcaggcgtc cggcagccag ccgatcacgt cgcgggcgtc gccttcgtgg atgcggacgt
       61 tcctgagggc ccgctcctcg atgtggcgca cagcgctggc cacgccgttg acgaagggct
      121 cggcgccgat gatcagggcg tgagggtgac ggccggcctg ggcggccatg tgctcgccgc
      181 cgccaaagcc gatctccagc caggcttccg tcgcctcggg catcaggtcg cgcggctcga
      241 acggcgcctc gggtacggcg atctccggca gcagggtgtc gagcagcgcc tgctggcgcg
      301 gcttcaccgg tcgcgacttg atgcggccga acgagcgcag cggcccgtga ggaatggtgg
      361 gggaggtcat gaggcggctc ttaccgcgag taggatcgcg tgcgaagcgc gaacaccaaa
      421 acatcgcctt cctcgccctt gtggcgagga cccatggcga ggcttggctc tgaatggaag
      481 aaggcgctcc gccagcgtgg tcgcgcgatg acgatctcaa cgctggcgga aacatgggtc
      541 ccaggcacaa ggcctgggaa ggcagatgag gttggcgttg ttaagccagg cccttcagct
      601 cgccgaccag atccgtcttc tcccacgaga agccgccctc gttgtccggc gtgcggccga
      661 agtggccgta ggcggcggtg cgggcgtaga tcggcttgtt gagctgcagg tgctcgcgga
      721 tcgagcgcgg ggtggcgccg ccgatcagtt gcggcaggat cttttccagc accgccggat
      781 caaccttgcc cgtgccgtgc aggtcgacgt ggaacgacac cggctgggcg acgccaatcg
      841 cataggcgca gcggtcggcc aggccggcgg ccacgacgtt cttggccagg tagcggcagg
      901 cataggcggc cgagcggtcg accttggtcg gatccttgcc cgagaacgcg ccgccgccgt
      961 gcggggccgc gccgccgtag gtgtcgacga tgatcttgcg gccggtgagg ccgcagtcgc
     1021 ccatcggccc gcccaccacg aagcgtccgg tggggttgac cagatagcgc gtgtcggcgg
     1081 tcagcatgcc tttcggcacc accggcttga tgatttcctc gatcaccgcc tcggtcagca
     1141 cggcgtgcga gacctcggga tgatgctggg tcgacagcac gatggtgtcg atcgaatgcg
     1201 gcttgccgtc gacgtagcgg atcgacacct gcgacttggc gtccggacgc agccacggca
     1261 ggcggccgtc cttgcgcagt tcggactggc gctcggtcag gcggtgcgcg aggtggatcg
     1321 gcatcggcat cagcaccgc
//
LOCUS       DAOMEO010001041         1336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10047_length_1336_cov_2.138173, whole genome shotgun sequence.
ACCESSION   DAOMEO010001041 DAOMEO010000000
VERSION     DAOMEO010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10047_length_1336_cov_2.138173"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLH31_18140"
     CDS             complement(<1..872)
                     /locus_tag="PLH31_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQR91147.1"
                     /translation="MKLTFKPLAGLMLAATLAGAPLGAVAFAQEAAPAVAAAPAEAAP
                     APEAAPAPEAAPAEAAPAAAAPVPDKGDNAWMLTSTVLVLLMILPGLALFYGGLVRSK
                     NMLSVMMQVSTVAIIGFVAWVLWGYSFAFTDGGSWDSYVGGLGRLFLKDVTPASNVAT
                     FSTGVVIPELSFIAFQATFAAITAALVVGALVERMKFAAIVAFAVLWPLLSYYPIAHM
                     VWWWPGPDAIAGDPTAPVKAGLIWSFGALDFAGGTVVHINAGIAALVGAIILGKRQGF
                     GKEPMPPHSLTLTLV"
     gene            complement(901..1239)
                     /locus_tag="PLH31_18145"
     CDS             complement(901..1239)
                     /locus_tag="PLH31_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQR91148.1"
                     /translation="MKLIIAIVKPFKLDEVRDALVAAGVEGLTVSEVKGYGRQKGQTE
                     IYRGAEYQVNFVPKVKLEAVVDDASAAKAVEAVKAAAATGKIGDGKIFVLNVEDAVRI
                     RTGETGPAAL"
BASE COUNT          224 a          444 c          458 g          210 t
ORIGIN      
        1 acaagggtca gggtcagcga gtgcggcggc atcggctctt tgccaaagcc ctggcgcttg
       61 cccaggatga tggcgccgac cagagccgcg ataccggcgt tgatgtggac cacggtgccg
      121 ccggcgaagt cgagggcgcc gaagctccag atcaggccgg ccttgactgg agcggtcgga
      181 tcacccgcga tggcgtccgg gcccggccac caccagacca tgtgggcgat cgggtagtag
      241 gagagcagcg gccacagcac cgcgaaggcg acgatggcgg cgaacttcat gcgctcgacc
      301 agggcgccga ccacgagggc ggcggtgatc gcggcgaagg tcgcctggaa cgcgatgaag
      361 ctgagctcgg ggatgacgac gccagtcgag aacgtcgcaa cgttgctggc cggggtcacg
      421 tccttgagga acagcctgcc aaggccgccg acatagctgt cccagctgcc gccgtcggtg
      481 aaggcgaagc tgtagcccca cagcacccag gcgacgaagc cgatgatggc gacggtcgag
      541 acctgcatca taaccgacag catgttcttg gagcgaacca ggccgccgta gaacagggcc
      601 aggccgggaa ggatcatcag caggaccagg acggtcgagg tcagcatcca ggcgttgtcg
      661 cccttgtccg gaaccggcgc agcggcggcg ggcgcggctt cggcgggcgc ggcttcaggc
      721 gctggagcag cttcgggcgc cggcgcggcc tcggcgggcg cggcggcgac ggccggggcc
      781 gcttcctggg cgaaggccac ggccccaagc ggggctccag ccagtgtcgc ggcgagcatc
      841 agcccggcca acggtttgaa ggtcaatttc atcgtcggtt atcccctgtt cccgatggcg
      901 ttaaagagcg gccgggccgg tttcaccggt ccggatgcgc acggcgtctt ccacgttcag
      961 aacgaagatc ttgccatcgc cgatcttgcc cgtggccgcc gcggccttca cggcctcgac
     1021 cgccttggcc gcggacgcat cgtccacgac cgcttccagt ttcaccttgg gtacgaagtt
     1081 cacctggtac tccgccccgc ggtagatttc ggtctggccc ttctggcggc cgtaaccctt
     1141 gacttccgac accgtcagac cctcgacgcc agcggcgacg agcgcgtctc ggacctcgtc
     1201 gagcttgaag ggtttgacga tcgctatgat cagtttcatc cgccttgctc cggcccgccg
     1261 atactgcggg ctccccttgc ttggttgccg aacgcgtcgc gtccggatga tcaccgccga
     1321 agccgccatt acgcgc
//
LOCUS       DAOMEO010001042         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10062_length_1335_cov_2.237500, whole genome shotgun sequence.
ACCESSION   DAOMEO010001042 DAOMEO010000000
VERSION     DAOMEO010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10062_length_1335_cov_2.237500"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLH31_18150"
     CDS             <1..985
                     /locus_tag="PLH31_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQR91149.1"
                     /translation="GIINAYGTNAKVGEGDWIVVEADESDGSFLKLKSTVAIVTNIDA
                     EHLDHWGTFDAVKKGFQDFIENIPFYGFAAVCTDHPEVQALAARIENRRLVTYGTNPQ
                     AEVRVSNIAMGPDGSTFDIIVSPLAGEPVRYDGLKMPMAGHHNVLNATAAVAVARELG
                     VDADAIRAGLASFGGVKRRFTTTGVANGIRVIDDYGHHPVEIAAVLKAARAVTPNGKV
                     IAVVQPHRFTRLRDLMTEFSSCFNDADTVIVADVYTAGESPIEGVDKDHLVEGLKKFG
                     HRRALPLENPTALPRLIASEATSGDLVVLLGAGDITTWAYALPGQLEALAK"
     gene            997..>1335
                     /locus_tag="PLH31_18155"
     CDS             997..>1335
                     /locus_tag="PLH31_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HQR91150.1"
                     /translation="MSWKADLPAVRGKLLFDEPLGPFTWFRVGGPAEVLFLPADADDL
                     RDFLRALPAQVPVQALGVGSNVIIRDGGVEGVVIRCAGRAFGEVTVDLEENLIIAGPA
                     ALDASVARAAA"
BASE COUNT          210 a          477 c          443 g          205 t
ORIGIN      
        1 cggcatcatc aacgcctatg gcaccaacgc caaggtcggc gagggcgact ggatcgtcgt
       61 cgaggccgac gagagcgacg gcagcttcct gaagctgaag tcgaccgtgg ccatcgtcac
      121 caatatcgac gccgagcacc tggaccactg gggcaccttt gacgcggtca agaagggttt
      181 ccaggacttc atcgagaaca tcccgttcta cggttttgcg gcggtctgca ccgaccatcc
      241 ggaggtccag gccctggccg cgcggatcga gaaccgccgg ctggtgacct acggcaccaa
      301 cccccaggcc gaggtgcggg tctccaatat cgccatgggc cccgacggct cgaccttcga
      361 catcatcgtc tcgcccctgg ccggagagcc ggtgcgctat gacggcctga agatgccgat
      421 ggccggccac cacaatgtgc tcaacgccac cgccgccgtg gccgtggccc gcgagttggg
      481 cgtcgacgcc gacgccatcc gcgccggtct ggccagcttt ggcggggtca agcgccgctt
      541 caccaccacg ggcgtcgcca atggcatccg cgtgatcgac gactacggcc accacccggt
      601 cgagatcgcc gccgtgttga aggccgcccg cgccgtgacc ccgaacggca aggtcatcgc
      661 cgtggtccag ccgcaccgtt tcacccgcct tcgcgacctg atgaccgagt tcagctcgtg
      721 cttcaacgac gccgacaccg tgattgtcgc cgacgtctac accgccggcg aaagccccat
      781 cgagggcgtc gacaaggacc atctggtcga gggcctcaag aagttcggcc accgccgggc
      841 cctgcccctg gaaaacccca cggccctgcc gcgcctgatc gccagcgagg ccaccagcgg
      901 cgatctcgtc gtgctgctgg gcgccggcga catcactact tgggcctatg cgctgccggg
      961 gcagctggaa gcgctggcca agtgagcggg ggcgaagtga gctggaaggc tgacctcccc
     1021 gctgttcgcg gcaagctgct gtttgacgag cccctggggc cgttcacctg gttccgcgtc
     1081 ggcggcccgg ccgaggtgct gttcctgcca gccgacgccg acgacctgcg cgacttcctc
     1141 agggccctgc cggcgcaggt tccggtccag gcgctgggcg tgggctccaa cgtcatcatc
     1201 cgcgacggcg gggtcgaggg cgtggtcatc cgctgcgccg ggcgggcctt tggcgaggtc
     1261 acggtcgatc tcgaggaaaa cctgatcatc gcgggcccgg cggcgctgga cgcctcggtc
     1321 gcccgggcgg cggcc
//
LOCUS       DAOMEO010001043         1335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10072_length_1335_cov_1.542969, whole genome shotgun sequence.
ACCESSION   DAOMEO010001043 DAOMEO010000000
VERSION     DAOMEO010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10072_length_1335_cov_1.542969"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PLH31_18160"
     CDS             <1..1295
                     /locus_tag="PLH31_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR91151.1"
                     /translation="LPEFYVSELGLSLGAVGAAFLIVRLIDIVFDPLIGGIMDRTRTQ
                     WGRFRPWLVAGSPLVMIGAFALFMAQPGIGPLYLWISLIVVYAGYSMMTLSHTAWAAV
                     LSTDYQQRSRVYGWWQAGNVVGMILVLTLPPIIALAFRGDHAAGIRSMGWFVVALIPL
                     TVLLATRAVGERQAPVTRHRSGLPQYFALLKRGSTRRLLLADLLMGLAPGIAGALFFF
                     FFERIKGFDKTEAGILLLVYFVAALAGAPLWPMLAKRIGKHQALAVASVIYAAVQVCA
                     VLTPAGNSLAGMLILILAGLPYSAAPLLVRAMMADIGDEERLASGADRTGLLYALVSG
                     TVKLGHALAIGAFPLLALFGFDPKLPTAAGETALVALYAVVPAAMGLVVAAVMLRYPL
                     DETRVAEIQRQLAVRDAATPSPQNPTPDPHVAFTAPAE"
BASE COUNT          175 a          451 c          416 g          293 t
ORIGIN      
        1 atctgcctga gttctatgtc agcgagcttg gcctatcgct tggcgcagtc ggtgcggcct
       61 tcctgatcgt tcgcctgatc gacatcgtgt tcgaccccct catcggcggg atcatggacc
      121 gcacgcggac ccagtggggc cgcttccggc cctggctggt ggcgggctca ccgctggtga
      181 tgatcggggc gtttgcgctg tttatggcgc agcccggcat cggcccgctc tatctctgga
      241 tcagcctgat cgtggtctat gcgggctatt cgatgatgac cctgtcgcac acggcctggg
      301 cggccgttct gtcgacagat taccagcagc gttcgcgagt ctatggttgg tggcaggccg
      361 gtaacgtcgt gggcatgatc ctggtcctga ccctgcctcc gatcatcgcg ctggccttca
      421 ggggcgacca tgccgcaggt atccggtcga tgggttggtt cgtcgtcgcg ttgatccccc
      481 tgaccgtgct tctcgcgacc cgcgcggttg gcgagcgcca ggctccagtg acccggcatc
      541 gatcaggctt gccgcagtac tttgcgctgc tgaagcgcgg ctcgacccgc aggctcctcc
      601 ttgccgacct gctgatgggc ctggcgccag gcattgccgg ggcgctgttc ttcttcttct
      661 tcgaacgcat taagggcttc gacaagaccg aagctggcat cctgttgctg gtctattttg
      721 tcgccgccct ggccggggcg ccgctttggc cgatgctggc caagcggatc ggcaaacacc
      781 aggccctggc ggtggcctct gtgatctatg ccgccgtcca agtttgcgcc gtcttgacgc
      841 ccgccggcaa ttccctggcg ggaatgttga tcctgatcct tgccggcctg ccctactccg
      901 ccgcacccct gctggttcgg gccatgatgg ccgacatcgg cgacgaggag cgactggcca
      961 gcggcgcgga ccggaccggc ctgctctatg cgcttgtcag cggtacggtg aagctgggcc
     1021 acgccctggc tatcggcgcc tttccgctgc tcgcgctgtt tggttttgac ccgaagctgc
     1081 ccacggcggc gggtgaaacc gccctcgtgg ctctctatgc cgtcgtaccc gccgccatgg
     1141 gccttgtggt cgccgccgta atgctgcgct atcccctaga tgagactcgt gtcgctgaaa
     1201 tccagcgcca gcttgccgtt agagacgctg cgacgccctc gccccagaac ccgacgccgg
     1261 acccgcatgt cgcctttaca gcccctgccg aatgatcccc agcatccgct tttcacgctc
     1321 atcgagatca tggcg
//
LOCUS       DAOMEO010001044         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10075_length_1334_cov_2.425332, whole genome shotgun sequence.
ACCESSION   DAOMEO010001044 DAOMEO010000000
VERSION     DAOMEO010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_1334_cov_2.425332"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLH31_18165"
     CDS             complement(<1..577)
                     /locus_tag="PLH31_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR91152.1"
                     /translation="MQHILEELDRRRAQARAGGGEKRVASQHAKGKLTARERIDLLLD
                     EDSFEEFDMFVEHRCADFGMQDQKIPGDGVVTGWGTINGKVVYVFSKDFTVFGGSLSG
                     AHAAKIVKVQRQAMKVGAPVIGLFDAGGARIQEGVDSLAGYADIFMENVLSSGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFV"
     gene            complement(712..>1334)
                     /locus_tag="PLH31_18170"
     CDS             complement(712..>1334)
                     /locus_tag="PLH31_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HQR91153.1"
                     /translation="TPKSPAKSPLILPSPALAQLVADEWEAQHKVIDSTIMPATRLAF
                     TAIDRIAETRAAVAAEVAAYAGSDLLCYRAEHPTPLVERQTREWQPVLDWAGRTLGVS
                     FAPVTGIIHTPQPPQTLIAVEALALSLDDFSLAGVAYAAGLLGSTVLALALRAGEMSG
                     QQALDLSRLEETFQADIWGQDDEARLRAHNLAIEALALERWFSALR"
BASE COUNT          214 a          452 c          459 g          209 t
ORIGIN      
        1 tcacgaacat gtagctggtg tccttcacca tgaagatgaa gtcggtcatg gccgggctgt
       61 agacatcgcc gccggcgcag gggcccatga tcacgctgat ctgcgggatc acgcccgaag
      121 acagcacatt ctccatgaag atgtcggcat agccggccag gctgtcgacg ccttcctgga
      181 tgcgcgcgcc gccggcgtcg aacaggccga tcaccggcgc gccgaccttc atggcctggc
      241 gctgcacctt gacgatcttg gccgcgtggg cgcccgaaag agagccgccg aacaccgtga
      301 aatccttgga gaagacgtag acgaccttgc cgttgatcgt gccccagccg gtgaccacgc
      361 cgtcgccggg gatcttctgg tcctgcatgc caaagtcggc gcagcggtgt tcgacgaaca
      421 tgtcgaactc ttcgaaggaa tcctcgtcca gcagcaggtc gatgcgctcg cgggccgtca
      481 gcttgccctt ggcgtgctgc gacgcgaccc gcttctcgcc gccacccgcg cgcgcctgcg
      541 cgcgacgacg atccagttcc tcgagaatgt gctgcacgga tggactcccc tgggcttgcc
      601 agcctcgtga tcacgggggc cgacttttcg gatcgagggg taaagccgcg cggtcgggct
      661 ggcaagccag cccttgcggc agcgcagcaa tgctctcgcc gctgatgatg ctcagcgcag
      721 cgccgagaac caccgctcca gggccagggc ttcgatggcc agattgtggg cccgcagccg
      781 cgcctcgtcg tcctggcccc agatatcggc ctggaaggtc tcctccagcc gcgacaggtc
      841 cagagcctgc tggccgctca tctcgcccgc gcgcagggcc agggccagaa cggtcgagcc
      901 cagcaggccg gccgcatagg ccaccccggc caggctgaag tcatccagcg acagcgccag
      961 ggcctcgacg gcgatcaggg tctgcggggg ctgcggcgtg tggatgatgc cggtgaccgg
     1021 tgcaaagctc acgcccagcg tccggcccgc ccagtccagc accggctgcc attcgcgggt
     1081 ctggcgctcg accagagggg tggggtgttc agcgcggtag cacagcaggt ccgagccggc
     1141 ataggccgcg acctcggcgg cgacagcggc ccgggtctcg gcgatgcggt cgatggcggt
     1201 gaaggccagg cgcgtggccg gcatgatcgt cgaatcgatg accttgtgct gcgcctccca
     1261 ctcgtcggcg accagctggg ccagggccgg actgggcagg atgagcggcg acttggccgg
     1321 cgacttgggc gtgc
//
LOCUS       DAOMEO010001045         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10087_length_1333_cov_2.316119, whole genome shotgun sequence.
ACCESSION   DAOMEO010001045 DAOMEO010000000
VERSION     DAOMEO010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10087_length_1333_cov_2.316119"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1333)
                     /locus_tag="PLH31_18175"
     CDS             complement(<1..>1333)
                     /locus_tag="PLH31_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQR91154.1"
                     /translation="VAAARPDAIMATGRSDFPNQVNNVLCFPFIFRGALDVGASEINE
                     AMKVAAVEAIAELARAEASEVVASAYGGVAPMFGAQYIIPKPFDPRLILQIAPAVARA
                     AMDSGVATRPIADFDAYRQELELFVYRSGQLMRPVFERARKAPLKVAYAEGEDERVLR
                     GVQTVLDEGLAHPVLIGRRDVIAAKVKEMGLRMDLGGRVQVIDPAADTELFAPLIERY
                     QALVGRRGVPPMAAERRVTGRRTVAASMLLQAGHVDAALVGGSGDWWQHMTYALPIIP
                     KRDDVSRVYAMSSLILQTGTLFFCDTHVNVDPTADQIAEVTLLAAEAVRRFGLTPKAA
                     LLSHSSFGASNSPTARKMREALSLVRERAPELEVDGEMHADAALSQHLRDRLVADSPL
                     KGSANLLVMPTLDAANISLTLLSAATESLLVGPILLGMSQPLHVLTPSVTAR"
BASE COUNT          209 a          447 c          477 g          200 t
ORIGIN      
        1 gcgcgccgtg acgctgggcg tcagtacgtg caggggctgg ctcatgccca gcaggatcgg
       61 gccgacgagc agggattccg tcgctgcgct gagcagggtc aggctgatat tggccgcatc
      121 cagggtcggc atgaccagca gattggccga gcccttcagc gggctgtcgg ccaccaggcg
      181 gtcgcgcagg tgctggctga gggcggcgtc ggcgtgcatc tcgccatcga cttccagctc
      241 cggcgcgcgc tcacgcacca ggctcagggc ctcgcgcatc ttgcgggcgg tcggtgaatt
      301 gctggcgccg aagctggaat gcgagagcag ggcagccttg ggcgtcaggc caaaccggcg
      361 cacggcctcg gccgccagca gggtaacctc ggcgatctgg tctgcggtcg ggtcgacatt
      421 gacgtgggtg tcgcagaaga acagcgtgcc cgtctgcagg atcagcgacg acatcgcata
      481 gacccggctg acatcgtccc gcttggggat gatcggcagg gcataggtca tgtgctgcca
      541 ccagtcgccg ctgccgccca ctagggccgc gtccacatgg ccagcctgca gcagcatcga
      601 ggcggcaacc gtgcggcggc ccgtgacacg gcgctcggcg gccatcggcg gtacgccgcg
      661 gcggccgacc agagcctggt agcgctcgat caatggcgcg aagagttcgg tgtcggcggc
      721 ggggtcgatc acctggacgc gaccgcccag atccatgcgc aggcccatct ccttgacctt
      781 ggcggcgatc acgtcacggc ggccgatcag gaccgggtgg gccagccctt cgtcgagcac
      841 ggtctggacg ccgcgcagca cgcgctcgtc ctcgccctcg gcataggcga ccttgagcgg
      901 cgccttgcgg gcccgttcga acaccggccg catcagctgg cccgagcgat agacgaaaag
      961 ctccagctcc tggcgatagg cgtcgaaatc ggcgatcggc cgcgtggcga cgccgctgtc
     1021 catcgccgcc cgcgccacgg ccggggcgat ctgcaggatc aggcgcggat cgaaaggctt
     1081 ggggatgatg tactgcgcgc caaacatcgg cgcgacgccc ccataggcgc tggccaccac
     1141 ttcggaggct tcggcccggg ccagttcggc gatggcctcg acggccgcca ccttcatggc
     1201 ctcattgatc tcgctggccc cgacatccag cgcgccccgg aagatgaagg ggaagcacag
     1261 gacgttgttg acctggttgg ggaagtcgct gcggccggtg gccatgatcg cgtcgggacg
     1321 cgctgcggcc acc
//
LOCUS       DAOMEO010001046         1332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10097_length_1332_cov_2.387627, whole genome shotgun sequence.
ACCESSION   DAOMEO010001046 DAOMEO010000000
VERSION     DAOMEO010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_1332_cov_2.387627"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLH31_18180"
     CDS             <1..569
                     /locus_tag="PLH31_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91155.1"
                     /translation="YWNFELRWRPKTVTKHQAEIAKILQESGWVSPGLTGPKLYMVSY
                     RDCPECVRFKAEAFPALHKAGVDTRVIEIARADENGAPKSTPVERATVAELWVNRSWA
                     LAQRWNATPVAAWTAPGVKPADGDIARTAVIEAGRANVEKLAPLLKDNGVGSGWPLMV
                     WWTKDGQMRACACEAPQTNRIVRKDLGA"
     gene            566..>1332
                     /locus_tag="PLH31_18185"
     CDS             566..>1332
                     /locus_tag="PLH31_18185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR91156.1"
                     /translation="MRALGWRDGCRAAAGLMVGVIAISGAALAQPAATTPAPLAALAR
                     SGAPQLTGRILVADATRILSDERAGANGDAPLWPWGSVSKQVAAAIAMTAVDRGELAL
                     DDPIAKRWNAVPTPTIGAVTVRQLLRHTAGLANPEDTPQTDGIPAFYLRRAVDAERDD
                     AVTFCAGPVKRPPETRFEYNNCDTIVLASVLGSAVRGGYEQLLAGRIALPLGLKSLRL
                     AKANERPIIANARGKAVAPVNLATFGAAGALLGTPEDL"
BASE COUNT          215 a          446 c          470 g          201 t
ORIGIN      
        1 gctactggaa tttcgagctg cgctggcggc ccaagacggt caccaagcac caggccgaga
       61 tcgccaagat cctgcaggag tcgggctggg tgtcgccggg gctgacggga cccaaactct
      121 acatggtcag ttaccgagac tgcccggaat gcgtgcggtt caaggccgag gcgtttccag
      181 cgcttcacaa ggcgggcgtc gacacccggg tgatcgagat cgctcgcgct gacgaaaatg
      241 gcgcgcccaa atccacgccg gtcgagcgcg ccaccgtggc cgagctctgg gtcaatcgca
      301 gctgggccct ggcgcagcgt tggaacgcga cgcccgtcgc ggcctggacc gcgccgggtg
      361 tcaaacccgc cgacggcgac attgcgcgca cggcggtgat cgaggctgga cgggccaatg
      421 tcgagaaact ggcccccttg ctgaaggata acggggtcgg ttcgggctgg cccctgatgg
      481 tgtggtggac caaggacggc cagatgcggg cctgcgcctg cgaggctcca cagaccaacc
      541 gcatcgtgcg gaaggatctg ggagcatgag ggccctgggt tggcgcgacg gctgcagggc
      601 ggcggcaggc ctgatggtcg gtgtcatcgc catcagcggc gctgcgctgg cgcagccggc
      661 cgcgacgact cccgcccctc tggcggcgct tgcccggtcg ggcgctcccc agctgacggg
      721 ccgaatcctg gttgccgatg cgacacggat cctgtccgat gaacgggccg gggccaatgg
      781 cgacgcgccg ctctggccgt ggggctcggt cagcaagcag gttgccgcgg cgatcgcgat
      841 gacggcggtg gatcggggcg aactggctct cgacgatccg atcgccaagc gctggaacgc
      901 cgttccgacg ccgaccatcg gcgcggtgac cgttcgccag ctcctgcgcc acaccgccgg
      961 cctggccaat cccgaggata cgcctcagac cgacggcatt cctgccttct atctgcggcg
     1021 cgccgttgac gcggaacggg atgacgccgt caccttctgc gccggaccgg tcaagcgccc
     1081 gcctgaaacc cgctttgagt ataataactg cgacaccatc gtgctcgcca gcgtgcttgg
     1141 atcggccgtg aggggcggct acgagcaact tctggccggc cggatcgcgc tgccgcttgg
     1201 gctgaagtcg ctccgcctgg ccaaggccaa tgagcggccg atcatcgcaa acgccagggg
     1261 caaggccgtg gccccggtca atctggcgac cttcggcgcc gccggcgcgc tactgggaac
     1321 gcccgaggat ct
//
LOCUS       DAOMEO010001047         1330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10125_length_1330_cov_2.554510, whole genome shotgun sequence.
ACCESSION   DAOMEO010001047 DAOMEO010000000
VERSION     DAOMEO010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_1330_cov_2.554510"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLH31_18190"
     CDS             complement(<1..759)
                     /locus_tag="PLH31_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91157.1"
                     /translation="MPDTRSDPCADSADGAALILAELKTSQGGAKGLAAAGGWVFLIN
                     DTNDFLAWQYGVSRWTPEDRSAVRNLLTERTRILADRCYQMVISPEKSVVYAEWLPGG
                     LDRLSIDPARPAVAMAQMFPNEVLYLAPRLEELKRLGLLFFRGDTHVNWLGAYHVYRE
                     TILALAARGAAVGTPIGFAQLGHYIAGMEGDILMQLPEAARPEFERGAVLGRVGAMLE
                     VTISHVLPESKRRARQVPTPGGYVPGGGGRETIIM"
     assembly_gap    844..853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<854..>1330)
                     /locus_tag="PLH31_18195"
     CDS             complement(<854..>1330)
                     /locus_tag="PLH31_18195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR91158.1"
                     /translation="VGKNVVLNATLWLQGSGTIVEIEDNCIIEGMIRVVRGEGGRIRI
                     GEGTTFGAVGISLHEAGEINIGKDCLFSTDIHMDVSDMHPIYDGTTRERINPAKSVQI
                     GDHVWLGQRVLLLKGATIGSGTVVGANSTVVGDLPNNVIAVGSPARVVRENIVWAR"
BASE COUNT          242 a          463 c          390 g          225 t
ORIGIN      
        1 catgatgata gtctcacgcc cgccgccgcc aggaacatag ccgcccggcg tcggaacctg
       61 gcgggcccgc cgtttcgatt cgggcagaac gtggctgatc gtgacctcca gcatggcgcc
      121 aacccgcccc aacacagcgc cgcgctcaaa ctcgggcctg gctgcttcag gcagctgcat
      181 gaggatatcg ccctccatgc cggcaatgta gtgccccagc tgggcgaagc cgataggcgt
      241 tccaacggcc gccccccgcg ccgccagagc caggatcgtc tcccgataaa cgtgataggc
      301 gcccaaccag ttgacgtgcg tatcgccgcg aaagaacagc agacccagcc gcttcagctc
      361 ttccaggcgc ggcgccagat acagaacctc gtttggaaac atctgcgcca tggccacggc
      421 cggacgggcg ggatcgatcg aaagcctgtc gagcccaccc ggcagccatt cagcatagac
      481 gacgcttttc tccggcgaga tgaccatctg atagcaacga tcagccagaa tccgggtccg
      541 ctcggtcagg aggttcctca cggccgagcg gtcctccggc gtccagcggc tgacgccata
      601 ctgccaggcc aggaagtcgt tggtgtcgtt gatcaggaag acccagccgc ccgcggcggc
      661 cagccccttg gctcccccct gcgaggtctt gagctccgcc agtatcaggg ccgcgccatc
      721 ggcgctatcg gcgcacggat cgcttcgcgt gtccggcatc tcagcgcttt cccaagccaa
      781 ggcgctgacg ccagccgccc gacgcgccgg aggttgggag ggcctgttca acgaagggct
      841 cgtnnnnnnn nnntgcgcgc ccaaacgata ttctcccgta cgacccgggc cggggagcct
      901 acggcgatga cattgttggg caggtctccg accacggtcg aattggcgcc aaccaccgtg
      961 ccggacccga tcgtcgcgcc cttgagcagc agcacccgct gccccagcca gacatggtcg
     1021 ccgatctgga cggacttggc gggattaatg cgctcacgcg ttgtcccatc atagatgggg
     1081 tgcatgtcgc tcacgtccat gtggatatcc gtcgagaaca ggcagtcctt gccaatattg
     1141 atttcgccag cctcgtgaag cgaaatcccc accgcgccga aggttgttcc ctcgccgatc
     1201 cggatccgcc cgccttcgcc ccgcacgacg cggatcatgc cctcgatgat gcagttgtct
     1261 tcgatctcga cgatcgttcc ggagccctgc agccatagag tggcgttgag cacgacattc
     1321 ttgccaaccc
//
LOCUS       DAOMEO010001048         1329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10136_length_1329_cov_2.361068, whole genome shotgun sequence.
ACCESSION   DAOMEO010001048 DAOMEO010000000
VERSION     DAOMEO010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_1329_cov_2.361068"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLH31_18200"
     CDS             complement(<1..403)
                     /locus_tag="PLH31_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91159.1"
                     /translation="MEDLFRSYWWLIFPLSWFVYGGFQSWLSYRANRDTLELLKSYAA
                     SGREPPPELVARLNKRWNYDLDDDRYDGPRRHRRERGWYHVVLFGVLCLGFTYAATSD
                     LYEAGPAFLIVAFVMGALSLASLVSVLTGRGP"
     gene            complement(427..609)
                     /locus_tag="PLH31_18205"
     CDS             complement(427..609)
                     /locus_tag="PLH31_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91160.1"
                     /translation="MLDLFHSFWWLLFPLSWVAVAGWNAWLAHKARRDAMDLIQTYVR
                     SGRDAPPELLARLNRA"
     gene            complement(657..>919)
                     /locus_tag="PLH31_18210"
     CDS             complement(657..>919)
                     /locus_tag="PLH31_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQR91161.1"
                     /translation="CGASLFGKARKFRKVTSGAARDRVICIGDETRDIEAARALGLAS
                     GAVTWGAAKRAILAEHGPTIVFESPSEIISHLVASRAAIGGD"
     assembly_gap    920..929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<930..1301)
                     /locus_tag="PLH31_18215"
     CDS             complement(<930..1301)
                     /locus_tag="PLH31_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQR91162.1"
                     /translation="MVIFDFDGTLADSAAWMMKTVNPMARRFGFRTVTDEEIDMLRGR
                     GTREVIRYLGISPWKLPMIASHGKKLMAAECESIPLFPETAGMLKALHDAGLRLAVVS
                     SNSERTIRKVLGPELSGLIDQF"
BASE COUNT          258 a          448 c          409 g          204 t
ORIGIN      
        1 tcgggcctcg gcccgtcagc accgacacca gcgaagccag gctcagcgcg cccatcacga
       61 aggctacgat cagaaaggcc ggaccagcct cataaaggtc ggatgtcgcg gcataggtga
      121 agcccaggca cagcacgcca aacagcacca catggtacca tccccgttca cggcgatgac
      181 gccgggggcc gtcgtagcgg tcgtcatcga gatcgtagtt ccagcgcttg ttcaggcgcg
      241 ccaccagctc cggcggcggc tcccgcccgc tggcggcgta ggatttgagc agttcgagcg
      301 tgtcgcggtt ggcgcgatac gacagccagc tctgaaaccc accgtacacg aaccaactca
      361 acggaaagat cagccaccag tacgaacgaa acaggtcttc catcgatttg cccctccgat
      421 agacgcctag gcccggttca acctggccag caactccggc ggcgcgtcgc gaccgctgcg
      481 cacataggtc tggatcaggt ccatcgcgtc gcgacgcgcc ttatgcgcca gccaggcgtt
      541 ccagccggcg acggcgaccc agctcagcgg gaacagcagc caccagaacg aatgaaacag
      601 gtccagcatc gtcatccctc ccatccgcgc gaggcgttcg cgccggcttc tgaaggttag
      661 tcgccgccga tggccgccct ggatgcgacg agatgagaaa taatttcgga cggggattca
      721 aagacgatcg tcgggccgtg ctccgccagg atcgcccgtt tggcggcgcc ccaggtcacc
      781 gcgccgctgg ccagacccag ggcgcgagcc gcttcaatat cgcgggtctc gtcgccaatg
      841 cagatcaccc ggtcgcgcgc ggcgccggag gtcaccttgc gaaacttccg cgccttgcca
      901 aacagcgagg cgccgcaggn nnnnnnnnng aactggtcga tcaggccgga aagctcaggc
      961 cccagcacct tgcggatcgt gcgctcgctg ttggagctga ccacagcaag tcgcagtccg
     1021 gcgtcgtgca acgccttcag catgccggcg gtctcgggaa acagcgggat ggactcgcac
     1081 tcggccgcca tcagtttctt gccgtgcgag gcgatcatcg gcagcttcca gggcgagatg
     1141 cccagatagc ggatgacctc ccgggtgccg cgcccccgca gcatgtcgat ctcttcgtcg
     1201 gtgacggtcc tgaagccgaa gcgccgggcc atgggattga cggtcttcat catccacgcc
     1261 gcgctgtcag ccagcgtgcc gtcgaagtca aagatgacca tgtcataaga cgggacgggg
     1321 taggctccc
//
LOCUS       DAOMEO010001049         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10147_length_1328_cov_1.812255, whole genome shotgun sequence.
ACCESSION   DAOMEO010001049 DAOMEO010000000
VERSION     DAOMEO010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10147_length_1328_cov_1.812255"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLH31_18220"
     CDS             complement(<1..232)
                     /locus_tag="PLH31_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HQR91163.1"
                     /translation="MNPDDQNLRGWEFWIDRGGTFTDIVARRPDGSLVTHKLLSENPE
                     HYADAAVAGVRALLPAGAPIDAVKMGTTVATNA"
     gene            complement(429..1004)
                     /locus_tag="PLH31_18225"
     CDS             complement(429..1004)
                     /locus_tag="PLH31_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQR91164.1"
                     /translation="MDSLLVSTLVVAIAEIGDKTQLLAMILATRFKKPVPIIFGILVA
                     TLANHALAATAGYWVADILSGQGFKWAIAVSFIAMAAWALIPDKADDEDGSSAGRYGV
                     FVTTTIAFFLVEMGDKTQIATVALGAKFHSIFWVALGTTLGMMIANIPAVYLGEAATK
                     VVPLKYVRIGAALIFLGLGVWAAVEAAGLSR"
BASE COUNT          226 a          446 c          446 g          210 t
ORIGIN      
        1 gggcgttggt ggcgacggta gttcccatct tgaccgcgtc gatgggcgcg ccggccggca
       61 gcagggcccg cacgcccgcc acggcggcgt cggcatagtg ttcggggttc tccgacagca
      121 gcttgtgggt gaccagcgac ccgtccggcc gccgcgccac gatgtcggtg aaggtgccgc
      181 cgcgatcgat ccagaactcc cagccgcgca ggttctgatc gtcgggattc atgaggtggc
      241 ggcgctccag ttgctcctga gcctcttacc ccttccgctc ttcccggcga aggccgggac
      301 ccagattcat ccagagagtt tggggtgggc gcgccatcgg cctcgacaag gtccaccacg
      361 cccagggttg gatctgggcc ccggcgttgg cttcgccgcc ctgcgggtcg ccggggagag
      421 cgggtgtgtt agcgggagag ccccgccgcc tcgaccgccg cccacacgcc cagccccaag
      481 aagatcagcg ccgccccgat ccgcacgtac ttgagcggca cgaccttggt cgccgcctcg
      541 cccagataca ccgccgggat attggcgatc atcatgccca aggtggtgcc cagggcgacc
      601 cagaagatcg agtggaactt ggcccccaaa gccaccgtgg cgatctgcgt cttgtcgccc
      661 atctcgacca gaaagaaggc gatggtggtg gtgacgaaca cgccgtagcg accggcgctg
      721 gagccatcct cgtcatcagc cttgtcgggg atcagggccc aggcggccat ggcgatgaac
      781 gagaccgcga tcgcccactt gaacccctgt ccgctcagga tatcggccac ccaatagccg
      841 gccgtggcgg ccagggcgtg gttagccagg gtggcgacca ggatgccgaa gatgatcggc
      901 acgggcttct tgaaccgtgt ggccaggatc atggccagca gctgggtctt gtcgccaatc
      961 tcggcgatgg ccacgaccag ggtcgaaacg agcagggagt ccatgggatc aaaaccttcc
     1021 gggccgggca cacgccaata gcgtcgacgt cccacccagc ccgggaggat atcggcgcca
     1081 ttggtctcgc catggaagcg gatgatcgct tccttccccg cgccatggcc tcaagagacc
     1141 aagtgtgttg acggcggggc ctgctgatgg atgcaggcgg ctactcccca aaggtgcgcg
     1201 gcgtttagcg gggcacacgg caagggtcaa gctcctcccc ctctggggga ggtggcccag
     1261 agggtcggag ggggccagca cggcataagc cgcattagcc ccctcagtcg ctccgcgaca
     1321 gctccccc
//
LOCUS       DAOMEO010001050         1327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10160_length_1327_cov_1.711478, whole genome shotgun sequence.
ACCESSION   DAOMEO010001050 DAOMEO010000000
VERSION     DAOMEO010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10160_length_1327_cov_1.711478"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLH31_18230"
     CDS             complement(<1..128)
                     /locus_tag="PLH31_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HQR91165.1"
                     /translation="MLRTALGADIAGYLDDPGVVEVMLNPDGRLWIDRLAGGLEDTG"
     gene            complement(313..756)
                     /locus_tag="PLH31_18235"
     CDS             complement(313..756)
                     /locus_tag="PLH31_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQR91166.1"
                     /translation="MKPPPRIKQTFRLPAELSRDLADYARRRRVAQGDVVQAALSSHL
                     SPDGADRLEAALARRLDRMTRQIDRLERHIMISNETLALFVRFWLTAVPPLPDSAQAA
                     AQAKGRERFEGFVETLGRRLAKGRSFADEIVQDVPATTSADPDEE"
     assembly_gap    831..840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<841..>1327)
                     /locus_tag="PLH31_18240"
     CDS             complement(<841..>1327)
                     /locus_tag="PLH31_18240"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQR91167.1"
                     /translation="LGTATEIRDARNYAGHRLSPWLGHLMVSRQETARPLLTPGEIMQ
                     LPPSDAIVLVSGLFPVRATKARYYEDRQLKARLLPPPTEPTQREEVGAQSDDWSALAP
                     LPFAPTAARTSGDDAIDGDADDSANAGIRREPEIPAHEAIAPETPLPVREFDALDDEP
                     DD"
BASE COUNT          227 a          413 c          440 g          237 t
ORIGIN      
        1 ccggtatcct ccaagccgcc cgcgagccgg tcgatccaca gacggccatc agggttgagc
       61 atgacttcga cgacgcccgg gtcatcgaga tacccggcga tgtcggcgcc cagcgcggtc
      121 cgcaacatcc gtgcgccgcg tgcgaaactc tcggaccgga aagcaactgt ggtcacgaac
      181 gtcgtcccgt tttcgcgtct cgccggaggc gagacagtca acgggtcgag caagaagcgg
      241 cgatatcgta ctgattcaat aggcttgagg gcgtagtagg gctgtagcgt acaatggcag
      301 ggaaacggcg gttcactcct cgtcgggatc tgcgctcgtg gttgccggaa cgtcctgaac
      361 gatctcgtcg gcgaagctgc gccccttggc gaggcgtcgc cccagggttt caacaaagcc
      421 ctcgaagcgt tcgcggccct ttgcctgagc tgccgcttgg gctgagtcgg ggagcggcgg
      481 cacggccgtc agccagaagc gcacaaaaag agccaatgtt tcgttgctga tcatgatgtg
      541 gcgctcaagc cggtcgatct ggcgggtcat gcgatcgagc cgtctcgcca gcgccgcctc
      601 caggcggtcg gcgccgtctg gtgataggtg ggatgacaat gcggcttgca ccacgtcgcc
      661 ctgggcgacc cgccgacgcc gggcatagtc agccaaatcc cggctcagct cagcgggaag
      721 gcggaatgtc tgtttgattc tgggaggcgg cttcacaata cgatcccatc atcaggatcg
      781 agagaagcct ggcgcgccat gccacgcatt cgcgcctgca gcgtctgagc nnnnnnnnnn
      841 gtcgtcaggt tcgtcgtcca gggcgtcgaa ctcgcgcacg ggcagcggtg tctccggcgc
      901 tattgcctca tgcgcgggaa tttccggctc ccgccggatt ccggcattgg cagaatcgtc
      961 ggcgtcgcca tcgatggcat catcccctga ggtccttgcc gcggtcggcg caaatggcag
     1021 cggcgccagc gcgctccaat catcgctctg cgcgccaacc tcctcgcgct gggtcggttc
     1081 ggtgggagga ggcagtagtc gcgccttgag ttggcggtcc tcatagtagc gcgccttcgt
     1141 cgcccggacc gggaagagtc ctgaaacgag cacgatcgca tcgctcggcg ggagctgcat
     1201 gatttcgccc ggggtgagca gcggccgcgc tgtttcctga cgcgaaacca tcaaatggcc
     1261 gagccagggc gacagacgat ggccggcata gttgcgggca tcgcggatct cggtcgcggt
     1321 tcccaag
//
LOCUS       DAOMEO010001051         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10178_length_1325_cov_2.306299, whole genome shotgun sequence.
ACCESSION   DAOMEO010001051 DAOMEO010000000
VERSION     DAOMEO010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_1325_cov_2.306299"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="PLH31_18245"
     CDS             complement(<1..101)
                     /locus_tag="PLH31_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQR91168.1"
                     /translation="MTAAPLIAPSILASDFAKLGAEVAAIEAAGADWV"
     gene            complement(162..>1325)
                     /locus_tag="PLH31_18250"
     CDS             complement(162..>1325)
                     /locus_tag="PLH31_18250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQR91169.1"
                     /translation="LEPRERAFARALAMATLRRLGPIDRALSAKLQKEPPPRVRNLLR
                     LGVVQAFYLDVPAFAAVATSVEMAGINKTSRPFKGLVNAVLRGLLRDGPPSDDPEQLA
                     PPWLFARWSGAWGLETARAVALEIANEPAADLSLKDPSLAESWAARLEGEILPGGTLR
                     ASRKGDVAAWPGFEEGGWWVQDAAAAIPARLLNVKPGETAVDLCAAPGGKSLQLAAAG
                     ARVVAVDRSAARLERLADNFKRMNLTAEVVAADAAAWDDARTFDAVLLDAPCSATGTF
                     RRHPDVLWAARPGDVASLAAVQSRLLDAAADRIKVGGRLVYCVCSLEPEEGEAQVEAF
                     LTRRPDMRLDPVTSGEAGAPAASLTARGTLRLLPHQLDGGQDGFFAARFVRHG"
BASE COUNT          182 a          457 c          515 g          171 t
ORIGIN      
        1 acccagtcgg cgccggccgc ctcgatcgcg gcgacctccg cgcccagctt ggcgaagtcg
       61 gaggcgagga tggacggggc gatgagcggt gcggcggtca tgcctttggc ttagccggcg
      121 tcgcgacgtg ggacaagcga gcgcatggtc gcgcctgccc tctaaccgtg cctgacgaac
      181 ctggccgcaa agaagccgtc ctgaccgcca tccagctgat ggggcagcag acgcagggtc
      241 ccgcgcgccg tcaggctggc ggccggcgcc ccggcctcgc ccgaggtgac cggatccagg
      301 cgcatgtcgg gccgccgggt caggaaggcc tcgacctggg cctcgccctc ttcgggttcc
      361 agcgagcaga cgcaatagac caggcgtccg ccgaccttga tcctgtcggc ggcggcgtcg
      421 agcagacgcg attggacggc ggcgaggctg gccacgtcgc cgggtcgcgc ggcccacagc
      481 acatcagggt ggcggcggaa ggtgccggtt gccgagcagg gcgcgtccag cagcacggcg
      541 tcgaaggtgc gggcatcgtc ccaggccgcg gcgtcggcgg ccacgacctc ggcggtgagg
      601 ttcatgcgct tgaaattgtc ggccagacgc tccagccggg cggccgagcg gtcgacggcg
      661 acgacgcgcg cgccggcggc cgccaactgc aggctcttgc cgccgggcgc ggcgcacagg
      721 tcgacggcgg tctcgccggg tttgacgttc agcagtcggg cggggatggc ggcggcggcg
      781 tcctgcaccc accaaccgcc ttcctcgaag cccggccagg cggcgacatc gcccttgcgg
      841 ctggcgcgca gcgtcccgcc gggcaggatc tcgccctcca ggcgcgcggc ccaggattct
      901 gcgaggctgg ggtctttcag ggagaggtcg gcggccggtt cgttggcaat ctccagggcg
      961 acggcgcggg cggtctccag accccaggcg ccgctccagc gggcgaacag ccacggcggg
     1021 gccagctgtt cgggatcgtc ggacggcgga ccgtcgcgca gcaggccgcg taacacggcg
     1081 ttgaccaggc ccttgaacgg gcggctggtc ttgttgattc cggccatctc gacgctggtg
     1141 gcgacggccg cgaaggcggg cacgtccaga tagaaggcct gcacgacgcc caggcgcagc
     1201 aggttgcgca cgcggggcgg cggctccttc tgaagctttg ccgacagggc gcggtcgatg
     1261 gggccgaggc gccgcagcgt ggccatggcc agggcgcggg caaacgcgcg ctcgcgcggc
     1321 tccag
//
LOCUS       DAOMEO010001052         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10210_length_1322_cov_3.236780, whole genome shotgun sequence.
ACCESSION   DAOMEO010001052 DAOMEO010000000
VERSION     DAOMEO010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10210_length_1322_cov_3.236780"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLH31_18255"
     CDS             <1..423
                     /locus_tag="PLH31_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR91170.1"
                     /translation="HAMATADATFSFSEVKLGLTPATISPYVIAALGPRTAKLLFATG
                     RVFDADAAWSYGLVDEVFDDVAGLEVARDALIEEMVACAPGAIGDAKALVNDFTDQKL
                     DKGLIEETAKRIARRRVSAEGQEGVRAFLARRKPSWTE"
     gene            484..>1322
                     /locus_tag="PLH31_18260"
     CDS             484..>1322
                     /locus_tag="PLH31_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQR91171.1"
                     /translation="MISSVLIANRGEIARRIIRTAKELGVETIAVYSEADADAPFVME
                     ADSAILIGAAPARESYLDPEKILAAARQMGAEAIHPGYGFLSENADFAQAVLDAGLVW
                     IGPPPSAIRAMGLKDAAKKVMIAAGVPTTPGYLGDDQSGERLAKEAAAIGYPVLIKAV
                     AGGGGKGMRKVLAAADFAEALASCQREAAASFGDQRVLLEKYVTRPRHIEVQVFGDSH
                     GNVVHLFERDCSLQRRHQKVIEEAPAPGMDEATRQAVCAAAVKAAKAVNYVGAGTVEF
                     IADA"
BASE COUNT          207 a          456 c          458 g          201 t
ORIGIN      
        1 cacgccatgg ccacggccga cgcgacgttc agcttctcgg aggtcaagct gggcctgacc
       61 ccggcgacca tcagccccta tgtgatcgcc gccttgggcc cgcgcacggc caagctgctg
      121 ttcgccaccg gccgggtgtt cgacgccgac gcggcctgga gctatggcct ggttgacgag
      181 gtgttcgacg atgtcgccgg cctggaagtc gcccgcgacg ccctgatcga ggaaatggtc
      241 gcctgcgccc ccggcgcgat cggcgacgcc aaggccctgg tcaacgactt caccgatcag
      301 aagctcgaca agggcctgat cgaagagacc gccaagcgca tcgcgcgtcg ccgcgtgtcc
      361 gccgaagggc aggagggcgt gcgcgcgttc ctggcgcgcc gcaagccgtc ctggacggaa
      421 tagcccggat ctttcgggcc ccgccgtttt ccactccgtc ccagatgtag gacttccttc
      481 gccgtgatct cttccgtcct catcgccaat cggggcgaaa tcgctcgtcg catcatccgc
      541 accgccaagg agctgggtgt tgagacgatc gccgtctact cggaagccga cgccgacgcg
      601 ccgttcgtga tggaggccga ttccgccatc ctgatcggcg cggcgccggc ccgggagagc
      661 tatctggacc cggaaaagat cctggcggcg gcaagacaga tgggcgccga ggcgatccat
      721 ccgggctatg gcttcctgtc cgagaacgcc gacttcgccc aggcggtgct ggatgcgggt
      781 ctggtctgga tcggcccgcc gcccagcgcc atccgcgcca tgggcctgaa ggacgccgcc
      841 aagaaggtca tgatcgcggc gggcgtgccc acgacgcccg gctatctggg cgatgatcag
      901 tcgggcgagc gcctggccaa ggaagccgcc gccatcggct atccggtgct gatcaaggcc
      961 gtggccggcg gcggcggcaa gggcatgcgc aaggtgctgg cggcggccga cttcgccgag
     1021 gccctggcct cgtgccagcg cgaggcggcg gccagctttg gcgaccagcg cgtgctgctg
     1081 gagaagtatg tcacccggcc gcgccatatc gaggtgcagg tgttcggcga cagccacggc
     1141 aatgtcgtcc atctgtttga gcgcgactgc tcgctgcagc gccgccacca gaaggtgatc
     1201 gaggaggccc cggcccccgg catggacgag gccacccgcc aggcggtgtg cgccgccgcc
     1261 gtcaaggccg ccaaggccgt aaactatgtg ggcgccggca cggtggagtt catcgccgac
     1321 gc
//
LOCUS       DAOMEO010001053         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10213_length_1322_cov_2.424625, whole genome shotgun sequence.
ACCESSION   DAOMEO010001053 DAOMEO010000000
VERSION     DAOMEO010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_1322_cov_2.424625"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLH31_18265"
     CDS             complement(<1..448)
                     /locus_tag="PLH31_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HQR91172.1"
                     /translation="MIRLILNILWFIMGGWLSGLLWLLGGFLLAITIVGLPYTGAAWR
                     IAGFAFWPFGKEIVSRELVAGKGDLGTGPLGFVLNVIWFIFAGWYIALSHLVIAFAEA
                     ITIIGIPFAIKDFQLALIALAPIGREVVEARSFTTLAIRCRAATSPR"
     gene            645..>1322
                     /locus_tag="PLH31_18270"
     CDS             645..>1322
                     /locus_tag="PLH31_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQR91173.1"
                     /translation="MGLFRKKRRPIELPTGVAPTLMEVKFRGCTFWMPEHWVITRQLD
                     DGKDYEPWVLDYFLQILKPGMTVLDVGASWGAFALPAARKVGSTGNGRVLLESAKASG
                     VANMQLLLVGVSDRLGTEYLRRQTVHNNFQMEPPTDGIPADLDAFDIAPVVPLDLLRA
                     DFGKVDVLKIDIEGMEYRAFMGARALMAEQKPITFLEYSPRFQSEISGADGSALLTFF
                     LDLGYGVE"
BASE COUNT          256 a          437 c          424 g          205 t
ORIGIN      
        1 ctctcggcga cgtcgctgcc ctgcacctta tagccaaggt tgtgaaggac ctcgcctcca
       61 ccacctcgcg gccgatcggg gccagggcga tcagggccaa ctggaagtcc ttgatggcga
      121 agggaatgcc gatgatggtg atcgcctcgg caaacgcgat gaccagatgg ctcagcgcga
      181 tgtaccagcc ggcgaagatg aaccagatga cgttgagcac aaagcccagc ggaccggtgc
      241 ccaggtcgcc cttgcccgcc acgagctcgc gactgacgat ctccttgccg aatggccaga
      301 aggcgaaacc ggcgatgcgc caggccgcgc ccgtataggg caggccgacg atggtgatgg
      361 ccagcaggaa gccgccgagc agccacagca ggcccgaaag ccagccgccc atgatgaacc
      421 acaggatgtt gaggatcagt cggatcacga tggcggctcc cagcccttga ccaagatcct
      481 agaggtggcg cgtgtcggcc gacttgtcga gaaccctgcg ggcgggttac ggcgaattca
      541 ccgcctcccc caaccctggg gagtatggcg cgccggccaa aatcggctag ttacctgcaa
      601 gcacaggacg ccggggcaaa tccgcgtcgc cgacgaggac gatcatgggc ctgttccgca
      661 agaagcgtcg accgatcgaa ctgccgaccg gcgtggcccc gaccctgatg gaagtgaagt
      721 tccgcggctg cacgttctgg atgcccgagc actgggtgat cacccgccag ctggatgacg
      781 gcaaggacta cgagccctgg gtgctcgact acttcctgca gatcctgaaa cccggcatga
      841 ccgtgctgga cgtcggggcc agctggggcg ccttcgccct gccggccgcc aggaaggtcg
      901 gctccaccgg taacggccgg gtgctgctgg aaagcgccaa ggccagcggc gtcgccaaca
      961 tgcagctatt gctggtgggc gtgtccgacc gcctgggcac cgaatatctg cgccgccaga
     1021 ccgtgcacaa caacttccag atggagccgc ccaccgacgg catccccgcc gacctggacg
     1081 ccttcgacat cgcccctgtc gtgccgctgg acctgctgcg cgccgatttt ggcaaggtcg
     1141 acgtgctgaa gatcgacatc gagggcatgg agtaccgcgc cttcatgggc gccagggccc
     1201 tgatggccga acagaagccg atcaccttcc tggagtattc gccgcgcttc cagagcgaaa
     1261 tttccggcgc ggacggatcg gccctgctga ccttcttcct cgacctcggc tatggcgtgg
     1321 aa
//
LOCUS       DAOMEO010001054         1320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10232_length_1320_cov_2.599209, whole genome shotgun sequence.
ACCESSION   DAOMEO010001054 DAOMEO010000000
VERSION     DAOMEO010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_1320_cov_2.599209"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLH31_18275"
     CDS             complement(<1..741)
                     /locus_tag="PLH31_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HQR91174.1"
                     /translation="MVAERPVLGLPRLPALPRGGVLTRAIGANIKSHVEREWFGSPPH
                     RLALSLPKPRGLAARPHDPRPVDPEHGRKILAGIMTLSGGALRLGEAGDPFDTPSPSR
                     RFALALHRFDWLPDLVAAGPDGARRALRLIDDWRRVFGRWNTFSWSGECLERRVFHLA
                     CATRVLAAEASDAEIADLSMDLARQARQLLKASDAPERVLERAVTATLAGCVLAEKPG
                     DQLIDQGLRIIAAQIDRIVLGDGGHATRS"
     gene            complement(750..>1320)
                     /gene="rpe"
                     /locus_tag="PLH31_18280"
     CDS             complement(750..>1320)
                     /gene="rpe"
                     /locus_tag="PLH31_18280"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQR91175.1"
                     /translation="DWVHVDVMDGHFVPNITLGPDIVKAIRPHASIPFDVHLMISPAD
                     PYLEAFRAAGADIISIHPEAGPHLHRSLKRIRELGAKAGVVFNPSTSIDHVEWILEDV
                     DLLLVMSVNPGFGGQSFIPGQLRKVEALRNMVDKAGLDIIIEVDGGVTPATAPQCVSA
                     GATALVAGSAVFKGGPGAYADNIRALKGG"
BASE COUNT          217 a          427 c          486 g          190 t
ORIGIN      
        1 cgagcgcgtg gcgtggccgc cgtcgcccag aacgatacgg tcgatctggg cggcgatgat
       61 ccttagccct tggtcgatca gctgatcacc gggcttctcg gccagcacac agccggccag
      121 agtggcggtg accgcgcgtt ccagcacccg ctcgggggcg tcagaagcct tgagcagctg
      181 gcgcgcctgg cgggccagat ccatcgacag gtcggctatt tcggcgtcgg aggcctcggc
      241 ggccaggacg cgggtggcgc aggccaggtg gaacacccga cgctccaggc actcgccgct
      301 ccagctgaaa gtgttccagc gtccgaacac ccggcgccag tcgtcgatca ggcgcagagc
      361 ccggcgggcg ccgtcagggc cggcggcgac caggtcgggc agccagtcga agcgatgcag
      421 ggccagggcg aagcgccggc tcgggctggg ggtgtcgaag ggatcgcccg cctcgcccaa
      481 ccgcagagcg ccgccagaca gggtcatgat cccggccagg atcttgcggc catgctctgg
      541 atcgaccggg cggggatcgt ggggacgcgc ggcgaggccc cgcggcttgg ggagggatag
      601 cgccagccgg tgcggcggcg aaccaaacca ttcgcgctcg acatggctct tgatattggc
      661 gccgatcgct cgcgtcagga cgccgccgcg cggaagcgcc gggagccttg gcaagccgag
      721 aacggggcgt tcggcgacca tcagggcgat cagccgccct tcagcgcgcg gatgttgtcg
      781 gcataggcgc cgggcccgcc cttgaacacc gccgagccgg cgactagggc tgtggccccg
      841 gcggagacgc attggggcgc cgtggccggg gtgacgccgc cgtcaacctc gatgatgata
      901 tccaggcccg ccttgtcgac catgttgcgc agggcttcca ccttgcgcag ctggccggga
      961 atgaagctct gaccgccgaa gccggggttc accgacatga ccagcagcag atcgacatcc
     1021 tccaggatcc actcgacatg gtcgatgctg gtcgagggat tgaagaccac cccggccttg
     1081 gcgcccagct cgcggatacg cttgagcgaa cggtgcaggt gcggaccggc ctcgggatgg
     1141 atgctgatga tgtcggcccc ggcggcgcgg aaggcctcga gataagggtc cgccgggctg
     1201 atcatcaggt ggacgtcgaa ggggatgctg gcatgaggcc ggatcgcttt gacgatgtcg
     1261 gggcccagcg tgatgttggg cacgaagtgg ccatccatca cgtcgacatg cacccagtcg
//
LOCUS       DAOMEO010001055         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10266_length_1316_cov_2.697859, whole genome shotgun sequence.
ACCESSION   DAOMEO010001055 DAOMEO010000000
VERSION     DAOMEO010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10266_length_1316_cov_2.697859"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1316
                     /locus_tag="PLH31_18285"
     CDS             <1..>1316
                     /locus_tag="PLH31_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR91176.1"
                     /translation="IHEKIRANYDRIPAGIAEPLIQTRGINDVPSLVLTLSPKPGAAG
                     QWTDQALYELAGKLRTEVAKVDNVGLTFIVGGRPEEIRIAPDPARLAQHGVSLGALMD
                     TVRQATRAFPAGQIRGGGQAIDVTAGRSLTSSTDIGLLALPSASGQAVYVRDVADVIQ
                     GPREDQARAWRYASVDGVWSQAPAASLAISKRKGANAVVVSQAVLARVEALKGSLLPD
                     SLDVAVTRDYGATANEKANELLFHLGLATLSIVVLIGLAIGWREAAVTAVVIPTTILL
                     TLFASNLMGYTINRVSLFALIFSIGILVDDAIVMIENIARHWAMGDGRSRIDAAVDAV
                     AEVGNPTVVATLTVVSALLPMLFVSGLMGPYMAPIPVNASAAMVFSFFVAVVIAPWLM
                     VRFARKTLAADGHGGGHDSEGKLGALYRQVAGRVIATRASAWTFLI"
BASE COUNT          196 a          479 c          423 g          218 t
ORIGIN      
        1 gcatccacga gaagatccgc gccaactacg atcgcatccc ggccggcatc gccgagccgc
       61 ttatccagac gcgcggcatc aatgacgtgc ccagtctggt gctgaccctg tcgcccaagc
      121 ccggggccgc cggccaatgg actgatcagg cgctttatga gctggccgga aagctgcgca
      181 ccgaggtcgc caaggtcgat aatgtcggcc tgaccttcat cgtcggcggc cggcccgagg
      241 aaatccgcat cgcccccgat ccggctcgcc tggctcagca cggcgtgtcg ctgggcgccc
      301 tgatggacac tgtgcgccag gccacccgag cctttcccgc cggccagatc cgcggcggcg
      361 gccaggcgat agacgtcacg gccggccgca gcctgaccag cagcaccgac atcggcctgc
      421 tggccctgcc ctcggccagc ggccaggcgg tctatgtccg cgacgtcgcc gacgtgatcc
      481 agggtccgcg cgaggatcag gcccgcgcct ggcgttacgc cagcgtcgac ggtgtctgga
      541 gccaggcgcc cgccgccagc ctggcaatct ccaagcgcaa gggcgccaac gctgtcgtgg
      601 tctcccaggc cgtgctggcc cgggtcgagg ctctgaaagg ctcgctcctg cccgacagcc
      661 tggacgtcgc cgtcacccgc gactatggcg caacggccaa tgagaaggcc aatgagctgc
      721 tgttccatct gggcctggcc accctgtcga tcgtggtgct gatcggcctg gccatcggct
      781 ggcgcgaggc ggcggtgacc gccgtggtca ttcccacgac catcctgctg accctcttcg
      841 cctccaacct gatgggctac acgatcaatc gggtcagcct gttcgccctg attttctcga
      901 tcggcatcct ggtcgacgac gccatcgtca tgatcgagaa cattgcccgc cactgggcca
      961 tgggcgacgg ccgaagccgg atcgacgcgg cggtcgacgc cgtggccgag gtgggcaacc
     1021 ccaccgtggt cgcgaccctg accgtggttt cggccctgct gccgatgctg ttcgtctcgg
     1081 gcctgatggg cccctatatg gcgccgatcc cggtcaacgc ctcggcggcc atggtcttct
     1141 cgttcttcgt cgccgtggtc atcgcgccct ggctgatggt gcgctttgcc cgcaagaccc
     1201 tggccgcaga cggtcacggc gggggccatg acagcgaagg caagctgggc gcactctacc
     1261 ggcaggtcgc aggccgcgtc atcgccaccc gcgcaagcgc ctggaccttc ctgatc
//
LOCUS       DAOMEO010001056         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10275_length_1315_cov_2.639683, whole genome shotgun sequence.
ACCESSION   DAOMEO010001056 DAOMEO010000000
VERSION     DAOMEO010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10275_length_1315_cov_2.639683"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..692)
                     /locus_tag="PLH31_18290"
     CDS             complement(249..692)
                     /locus_tag="PLH31_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR91177.1"
                     /translation="MTDQAIQIGELSRRTRCNIETIRYYERIGLMPAPPRRGRYRSYG
                     AEDVGRLGFVRRARELGFTLDEVRALLRLASGGEASCAEVRTLAASHLTDVRARIADL
                     RRMERVLADSVRACDAGQDSGCPLIQSLYAERGQSSDSLDTKIPM"
     gene            1187..>1315
                     /locus_tag="PLH31_18295"
     CDS             1187..>1315
                     /locus_tag="PLH31_18295"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HQR91178.1"
                     /translation="MSHVQIEARSTLTCPHCGHRATETMPTDACQYFYDCLGCGAVL"
BASE COUNT          173 a          451 c          453 g          238 t
ORIGIN      
        1 accacggtcg ggcgcgccgc ttagacgcca gcgcggcctt caccggatgg gtactgggca
       61 agatagctcg gccgagtttc tgcgcagcct gccccgcaac gccccggaaa cggagagcgg
      121 ccggcgggcg tgctcggtcc cgccgcgtgc cgcggctcct acgggttcgc ccctggcggg
      181 cgccttcagg caccgccggc cgctcgacgg cgcgtcccgc ctggcctgcg tccaggctcc
      241 ttgcagcctc acatggggat tttagtgtca aggctgtcgc tcgactgccc gcgttccgca
      301 tatagggact ggatcagcgg acagcctgag tcctgtccag cgtcacaggc tcgcaccgag
      361 tccgccagga ctcgctccat gcgcctgagg tcggcgatcc gcgcgcggac atccgtcagg
      421 tgcgacgcgg ccagggtcct cacctcggcg caggacgctt ctccgccgct ggcgaggcgc
      481 agtagggcac gcacctcgtc cagcgtgaag cccagttcgc gcgcccggcg cacgaagccg
      541 agccggccga cgtcttcggc gccatagctg cgatagcgtc cccgccgcgg cggggctggc
      601 atcaggccga tccgctcgta gtagcggatc gtctcgatat tgcagcgtgt ccggcgtgag
      661 agctcgccga tctggatcgc ctggtcggtc atcggagcat gactttacgt ttagggcttg
      721 agtctgtagc cgctacagat ggcatcctcg ttctcatggc gccaggagac gatgacatga
      781 cggcgagtag cgggcttcga gcacgaccgg acggagatgg cgggaagcct gccgagaccg
      841 ccctgagctg ggccgccgtt gtggctgcgg cgggtgcggt cttcgcctgg gccgcctgct
      901 gcgtcttgcc gatgtccctc gctctagcag ggctaggcct cggaagtttt ggatggatcg
      961 ccggtcagcg gacttggatc acgttcgccg cgctgggcgt ggtcggcgtg ggttgggttc
     1021 tgacttggcg gcgtgcccga atgtgtcgca ccgatgccgc ctgtcgcgca ccgtcgcggt
     1081 tggggaccgc catgttgggc gtggccacgg ccctctgcct cctcgccctg acctggcagc
     1141 ccattgttga gccctgggcg ctgtccctca tccggagcgc ccgcggatga gccacgtgca
     1201 gatcgaggcc cgctcgaccc ttacctgtcc gcactgcggt caccgggcga ccgagactat
     1261 gccgaccgac gcctgccagt atttctacga ctgcttaggt tgcggcgccg tcctg
//
LOCUS       DAOMEO010001057         1315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10277_length_1315_cov_2.523810, whole genome shotgun sequence.
ACCESSION   DAOMEO010001057 DAOMEO010000000
VERSION     DAOMEO010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_1315_cov_2.523810"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1315
                     /gene="fusA"
                     /locus_tag="PLH31_18300"
     CDS             <1..>1315
                     /gene="fusA"
                     /locus_tag="PLH31_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQR91179.1"
                     /translation="VDFTIEVERSLRVLDGAVTVLDGNAGVEPQTETVWRQADKYKVP
                     RIVFVNKMDKIGADFDKSVESIRDRLGAKAVPIQFPIGSETNLKGLVDLVRMKAVIWD
                     NDALGANFRDEEIPADLMDKAVEARAYLVENAVEMDDEAMEAYLGGEEPSEEVIKKCL
                     RKAVLEGAFYPILCGSAFKNKGVQTLLDAVVDYLPSPLDIPPTKGIDFKTEEEIVRLA
                     SDEEPLSVLAFKIMDDPFVGSLTFCRIYSGKLETGMGLLNATRDKRERVGRMLLMHSN
                     NREDIKEAFAGDIVALAGLKDTRTGDTLCDPLKSPVILERMEFPAPVIEIAVEPKSKA
                     DQEKLGVALQKLAAEDPSFTVSTDFESGQTILKGMGELHLDIKIDILKRTYKVEANIG
                     APQVAYRESLGRKVDIDYTHKKQTGGTGQFARVMITFEPGEPGSGF"
BASE COUNT          272 a          418 c          407 g          218 t
ORIGIN      
        1 cgtcgacttc accattgaag tcgagcgttc gctgcgcgtg ctcgacggcg ccgtgacggt
       61 gctggacggc aacgccggtg ttgagccgca gacggaaacc gtctggcgtc aggccgacaa
      121 gtacaaggtt ccgcgtatcg tcttcgtcaa caagatggac aagatcggcg ccgacttcga
      181 caagtcggtc gaatcgatcc gtgaccgtct gggcgccaag gccgtgccga tccagttccc
      241 gatcggttcg gaaaccaacc tgaagggctt ggtcgacctg gtccgcatga aggctgtcat
      301 ctgggacaac gacgccctgg gcgccaactt ccgcgacgag gaaatccccg ccgacctgat
      361 ggacaaggcc gtggaggccc gcgcctatct ggtcgagaac gccgtcgaaa tggacgacga
      421 ggcgatggaa gcctatctgg gcggcgaaga gccctcggaa gaagtgatca agaagtgcct
      481 gcgcaaggcc gtccttgagg gtgcctttta tccgatcctc tgcggctcgg ccttcaagaa
      541 caagggcgtg caaacgctcc tggacgccgt tgtcgactac ctgccgtcgc cgctggacat
      601 cccgccgacc aagggcatcg acttcaagac cgaagaagag atcgtgcgtc tggcctcgga
      661 cgaagagccg ctgtcggttc tggcgttcaa gatcatggac gaccccttcg tgggctcgct
      721 gaccttctgc cgcatctatt cgggcaagct ggaaaccggc atgggcctgc tgaacgccac
      781 gcgcgacaag cgcgagcgcg ttggccgcat gctgctgatg cactccaaca accgcgaaga
      841 catcaaggaa gcctttgccg gcgacatcgt cgccctggcc ggcctcaagg acacccgcac
      901 cggtgacacc ctgtgcgatc cgctgaagtc cccggtgatc ctggagcgca tggaattccc
      961 ggcgcccgtc atcgaaatcg ccgtcgagcc caagtcgaag gccgaccagg agaagctggg
     1021 cgtcgccctg cagaagctgg ccgccgaaga tccgtccttc accgtctcga ccgacttcga
     1081 aagcggccag acgatcctga agggcatggg cgaactgcac ctggacatca agatcgacat
     1141 cctgaagcgg acctacaagg tcgaagccaa tatcggcgcg ccgcaggttg cctatcgcga
     1201 aagcctgggc cgcaaggtcg acatcgacta cactcacaag aagcagaccg gcggtacggg
     1261 ccagttcgcc cgcgtcatga tcacctttga gccgggcgag ccgggttcgg gcttc
//
LOCUS       DAOMEO010001058         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10290_length_1314_cov_2.072280, whole genome shotgun sequence.
ACCESSION   DAOMEO010001058 DAOMEO010000000
VERSION     DAOMEO010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10290_length_1314_cov_2.072280"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLH31_18305"
     CDS             complement(<1..350)
                     /locus_tag="PLH31_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR91180.1"
                     /translation="MKMNAQLSASASAARAHQEMFAYWASLRRGAALPARADLNPLGI
                     KRMLPTVSLIDVVGEPRDYRLRLAGTGLYGVYGREITGKGLSDVYNTAAADYWRKELD
                     KVVDGRRPGVGVHS"
     gene            complement(546..965)
                     /locus_tag="PLH31_18310"
     CDS             complement(546..965)
                     /locus_tag="PLH31_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HQR91181.1"
                     /translation="MDDQSDLLDMTAGIVSAYVGNNTVTASDVPALIRQVHSALAAVG
                     GPAEVEPAPPKEPAVPVKRSIAPDHLVCLEDGRKFKSLKRHLRTKYDMSPEEYRAKWN
                     LPKDYPMVAPNYAKARSELAKSMGLGQGGRKPPRGGK"
     gene            complement(1165..>1314)
                     /locus_tag="PLH31_18315"
     CDS             complement(1165..>1314)
                     /locus_tag="PLH31_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91182.1"
                     /translation="VLRYWNWSLSSALTPALLKAIREGDEAAVDEYSVPQRAAMLALS
                     RDFGR"
BASE COUNT          257 a          425 c          399 g          233 t
ORIGIN      
        1 atgctgtgga cgcccacgcc gggacggcgg ccatcaacca ccttgtccag ctccttgcgc
       61 cagtagtcgg cggcggcggt gttgtagacg tctgacagac ccttgccggt gatctcgcgg
      121 ccatagaccc cgtagaggcc ggtgccggcc agacgcaggc gatagtcacg cggctcgccc
      181 accacgtcga tcaggctgac ggtcggcagc atgcgcttga tgccaagcgg attcaggtcc
      241 gcgcgcgccg ggagggcagc cccccgtcgg agcgacgccc agtaggcgaa catctcctgg
      301 tgagcgcggg ccgccgaggc cgatgcgctc aactgcgcgt tcatcttcag attccttaac
      361 aagccgttgg aatcacgacg attcactatc gcttaaggac cgaatcgcga gcaagcaaaa
      421 acgacgccgc cagccaagat ttcaacaaag agtcaaccaa caagcgaaac gcccgccaac
      481 cgactcggct gacgggcgtc gcttatatct ggccgcgcaa acggcaagcg cttgatccga
      541 ctcggctatt tcccgccgcg cggcggtttg cggccgccct ggcccaggcc catcgacttg
      601 gccagctcgg accgcgcctt ggcgtagttg ggcgccacca tcgggtagtc cttgggcagg
      661 ttccacttgg cccggtactc ttcggggctc atgtcgtact tggtgcgcag gtggcgcttg
      721 agcgacttga acttgcgacc gtcctccaga cagaccagat gatccggcgc gatggagcgc
      781 ttgaccggca ccgccggctc cttgggcggc gcgggctcga cctcggcggg accgccgacg
      841 gccgccaggg cgctgtggac ctgccggatc agcgccggaa catccgacgc ggtcaccgta
      901 ttgttgccga cataggccga gacgatgccc gccgtcatgt cgaggaggtc cgattggtcg
      961 tccatctgat ctaatccgcc gccgctctag tttgttcttg tctgattccg taatcagtca
     1021 tcgcgtgaat acgcgaaaac agccagacgc ggcaatatcc agaaatgcag aataaacacc
     1081 aactgatcca gcacgtcgca cacggaggtt gagtttatca acgctccgca acaattggcc
     1141 gaatgcgttc aatgatattt gcggttaacg gccaaaatcg cgtgatagcg ctagcatcgc
     1201 ggcgcgctgg ggcaccgaat attcgtccac agccgcctcg tcgccttcgc ggatggcttt
     1261 caacagggcc ggcgtcaggg cagaggacaa cgaccagttc cagtagcgca gcac
//
LOCUS       DAOMEO010001059         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10309_length_1311_cov_2.794586, whole genome shotgun sequence.
ACCESSION   DAOMEO010001059 DAOMEO010000000
VERSION     DAOMEO010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10309_length_1311_cov_2.794586"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PLH31_18320"
     CDS             <1..1308
                     /locus_tag="PLH31_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR91183.1"
                     /translation="RFNDWAWAPRADGQRHRYFHPFEAPFSTDGTSLTPYWLLQDEAT
                     RAPFAEAMTIQARVAEAQRAPKRPHEGNFSGPLNYAYHFDAAKLANVLAERARELGVR
                     HLQGLLTGVALDETGAIASVSCQQHGTLEADLYIDCTGFRAELIGQALNSPFKSVRST
                     LFADRALACKIVQDGPETPIQSFTVATAHQAGWIWDIGLNGARGVGCVYSSDHMADDQ
                     AEAALRGYVGDGVEITPRSLAFDAGYRQKPWIKNCVAVGLSAGFLEPLESTGVVLIEA
                     AVAIIAELFPHNGPIAAPARRFNELMTARYDNIITFLKLHYCLSQRTEPFWRDNVDEA
                     SIPERLIDLLEQWRYRPPTRYDFTLDLETFAFFNYQYILYGMGFKTDLSPGRAEFPDV
                     AGAEKLFAKIRGFGERATHDLPSHRDLIAQINQFGFDRSLEPA"
BASE COUNT          213 a          464 c          413 g          221 t
ORIGIN      
        1 cgcttcaacg actgggcctg ggcgcccagg gccgacggcc agcgccaccg ctacttccat
       61 ccgttcgagg ccccgttctc gaccgatggg accagcctga ccccttactg gctgctgcag
      121 gacgaggcca cccgagcgcc gttcgccgag gccatgacca tccaggcccg cgtcgccgag
      181 gcccagcggg cgcccaagcg tccgcacgag ggcaacttct ccgggccgtt gaactacgcc
      241 tatcatttcg acgccgccaa actggccaat gtgctggccg agcgcgcgcg ggagctgggg
      301 gtgcggcatc tgcagggcct gctgaccggc gtggccctgg acgagaccgg cgccatcgcc
      361 tcggtctcct gccagcagca cggaacgcta gaggccgacc tctatatcga ctgcaccggc
      421 tttcgcgccg agctgatcgg ccaggcgctg aattcgccgt tcaagtcggt acgctcaacc
      481 ctgttcgctg accgggccct ggcctgcaag atcgtccagg acgggcccga gacgccgatc
      541 cagagcttca cggtcgcgac cgcccaccag gccggctgga tctgggacat cggcctgaac
      601 ggcgcgcggg gggtgggctg cgtctattcc agcgatcaca tggccgacga tcaggccgag
      661 gcggctctgc gcggctatgt cggcgacggc gtcgagatca ccccgcgctc gctggcgttc
      721 gacgccggtt atcgccagaa gccgtggatc aagaattgcg tcgccgtggg cctgtcggct
      781 ggcttccttg agccgctgga atccaccggc gtggtgctga tcgaggcggc ggtggcgatc
      841 atcgccgagc tcttcccgca caatggcccg atcgccgcgc cggcccggcg gttcaacgag
      901 ctgatgacgg cgcgctacga caacatcatc accttcctga agctgcacta ttgcctcagc
      961 cagcggaccg agccgttctg gcgcgacaat gtcgatgagg cttcgatccc cgagcgcctg
     1021 atcgatctgc tggaacaatg gcgctatcgg ccgcccaccc gctacgattt caccctggat
     1081 ctggagacct tcgcgttctt caactaccag tacatcctgt acggcatggg cttcaaaacc
     1141 gacctgtcgc cggggcgagc tgagtttccc gacgtggccg gggcggaaaa gctgttcgcc
     1201 aagatccgcg gctttggcga gcgcgccacc cacgatctgc ccagccaccg cgacttgatc
     1261 gcccagatca accagttcgg cttcgaccgc tcgctggagc ccgcctgatc g
//
LOCUS       DAOMEO010001060         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10311_length_1311_cov_2.544586, whole genome shotgun sequence.
ACCESSION   DAOMEO010001060 DAOMEO010000000
VERSION     DAOMEO010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10311_length_1311_cov_2.544586"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLH31_18325"
     CDS             <1..276
                     /locus_tag="PLH31_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HQR91184.1"
                     /translation="SLDGGVAAALLCLFMSSASAFYVEKVAQLGATYGSISAVIIFLI
                     WLSWSVNAIFLGGALATEVEIALDERPRALVEGPRGVPLKTPSTPES"
     gene            complement(248..478)
                     /locus_tag="PLH31_18330"
     CDS             complement(248..478)
                     /locus_tag="PLH31_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HQR91185.1"
                     /translation="MSEPILVDARGHRCPVPTLRLRRALEAAPAGGQVRLIADDPLAR
                     IDVPHFAASIGATVISTTEEGGAISFLVSKAS"
     gene            complement(471..>967)
                     /locus_tag="PLH31_18335"
     CDS             complement(471..>967)
                     /locus_tag="PLH31_18335"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HQR91186.1"
                     /translation="SHKEDGERIYDLLPRRWRIRDPGLSTVGRLDKDTSGLILITDDG
                     DYLHRVISPKRHVPKTYLATLDRPLKGSEGAIFSSGELMLEGEEKPLLPAELAVIDPH
                     HARLTITEGRYHQVRRMFAAVGNHVLELHRERIGGLVLPSDLEPGQHRILTAAEAEKV
                     FGDE"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<978..1169)
                     /locus_tag="PLH31_18340"
     CDS             complement(<978..1169)
                     /locus_tag="PLH31_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQR91187.1"
                     /translation="MTKALMARLDRLLANLGYGSRKDVQALISSGKVMLDGRVLKDVG
                     TKVAVTADLPERMSIRGRPV"
     gene            complement(1166..>1311)
                     /locus_tag="PLH31_18345"
     CDS             complement(1166..>1311)
                     /locus_tag="PLH31_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR91188.1"
                     /translation="AASLRKGGDLWLVANRHLPYEAILNEQFAKVRLAGETNGYKVYE
                     ARK"
BASE COUNT          205 a          426 c          429 g          241 t
ORIGIN      
        1 tccctcgacg gcggcgtcgc ggcggcgctg ctctgcctgt tcatgtcttc ggccagcgcc
       61 ttttatgtcg agaaggtcgc gcaactgggc gcgacctatg gctcgatttc ggcggtgatc
      121 atttttctga tctggctgtc gtggagcgtg aacgccatct tcctgggcgg cgccctggcg
      181 acggaggtcg aaatcgcgct ggatgaacgc ccgcgcgccc tggtcgaggg gccgcgcggc
      241 gtgccactca agacgccttc gacaccagaa agctgatggc gccgccttcc tcggtcgttg
      301 aaatcaccgt tgcgccgatg ctggcggcga agtgcggcac gtcgatgcgc gccagggggt
      361 cgtcggcgat cagccgcacc tgcccgcccg ccggcgccgc ttccagggcc cgccgcaacc
      421 gaagcgtcgg caccggacag cgatgccctc gggcgtcgac gaggatcggt tcactcatca
      481 ccgaacacct tttcggcctc ggcggcggtc aggattcgat gctgcccggg ttccagatcg
      541 gagggcaaca caaggccgcc aatgcgttcc cggtgaagtt cgagcacgtg gttgccgaca
      601 gccgcgaaca tgcgccgcac ctggtgatat cgcccttcgg tgatggtcag gcgggcatga
      661 tgcggatcga tgacggccag ctctgccggc agcagcggct tttcctcgcc ttccagcatc
      721 agctcgcccg atgagaaaat cgcaccttcc gaacccttta gcggccgatc cagcgtcgcg
      781 agataggtct tgggcacatg gcgtttgggc gagatcaccc tgtggagata gtcgccatcg
      841 tcggtgatca gaatcaggcc cgaggtgtcc ttgtccagtc gcccgacggt cgacagcccc
      901 gggtcgcgaa tgcgccagcg acggggcaga aggtcataga tccgctcgcc gtcttccttg
      961 tgcgagcnnn nnnnnnngac agggcggccc cggatgctca tgcgctcggg cagatcggcg
     1021 gtgacggcga ccttggtccc gacgtctttc agaaccctgc cgtccagcat aaccttgccg
     1081 ctcgagatca gcgcctggac gtccttgcgg ctgccgtagc ccagattggc cagcagccgg
     1141 tcaaggcggg ccatcagggc cttggtcact tgcgggcctc atagaccttg taaccattgg
     1201 tctcgcctgc gagacggacc ttggcgaact gctcgttcag aatggcctcg tagggcaggt
     1261 gccggttggc cacgagccac aggtcaccgc ccttgcgcag gctggcggcg g
//
LOCUS       DAOMEO010001061         1310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10321_length_1310_cov_2.243825, whole genome shotgun sequence.
ACCESSION   DAOMEO010001061 DAOMEO010000000
VERSION     DAOMEO010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10321_length_1310_cov_2.243825"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLH31_18350"
     CDS             complement(<1..464)
                     /locus_tag="PLH31_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQR91189.1"
                     /translation="MALKVAIVGLPNVGKSTLFNALTQTASAQAANYPFCTIEPNTGE
                     VAVPEPRLDALAKVAGSKEIIPARITFVDIAGLVRGASKGEGLGNQFLANIRDCDAVA
                     FVARCFEDGDIVHVEGRIDPISDLDIIETELMLADLESLEKRVPALEKRAKTG"
     gene            584..>1310
                     /locus_tag="PLH31_18355"
     CDS             584..>1310
                     /locus_tag="PLH31_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQR91190.1"
                     /translation="MSIRPVVKIVNGVPASDGDGVRLTRMLGTAEAQMFDPFLMLDCF
                     DNDQASDYMGGFPDHPHRGFETVTYMLEGRMRHRDNTGREGIIGPGGIQWMRAGRGIV
                     HSEMPEQSEGRMRGFQLWVNLPARLKMSAPGYSEFESDAIPVEARDGGVSVKVISGAT
                     EGGTAGPIGGGAVDALYFDVALPAGTRFEEPVGGDRNAMVAVYEGQVRVAGGKIDALS
                     GVFLGAGDRVVVEAVTDARVLLLA"
BASE COUNT          211 a          402 c          479 g          218 t
ORIGIN      
        1 ccggtcttgg cgcgcttttc cagggccgga acgcgctttt ccaggctttc aaggtcggcc
       61 agcatcagct cggtctcgat gatgtcgaga tcgctgatcg ggtcgatgcg gccttccacg
      121 tggacgatgt cgccgtcctc gaagcagcgg gccacgaagg ccacggcgtc acagtcgcgg
      181 atgttggcca ggaactggtt gcccaggccc tcgcccttgg acgcgccgcg caccaggccg
      241 gcgatgtcga cgaaggttat ccgggccggg atgatttcct tggagccggc gaccttggcc
      301 agggcgtcaa gacggggctc gggcaccgcg acctcgccgg tgttgggctc gatcgtgcag
      361 aacggatagt tggcggcctg ggccgacgcc gtctgggtca gggcgttgaa cagggtggac
      421 ttgccgacat tgggcaggcc gacgatggcg actttaaggg ccatggaact ctcgtgaatt
      481 tcgttggccg cgacctagca cggatcgata cggaacgcga agggcggggg cttgcaagaa
      541 ctgtaaccgg ttccgatatt gattgcggaa aaggagcagc gccatgagca ttcgacccgt
      601 cgtcaagatc gtgaacggcg tccccgcctc ggatggcgac ggcgtgcgcc tgacccgcat
      661 gctgggcacg gcggaggccc agatgttcga cccgttcctg atgctggact gtttcgacaa
      721 cgaccaggcg tccgactaca tgggcggctt tccggaccac ccgcatcgcg gcttcgagac
      781 cgtgacctac atgcttgaag gccggatgcg ccaccgcgac aataccggcc gcgaggggat
      841 catcggcccc gggggcatcc agtggatgcg ggccggcagg ggcatcgtcc attccgaaat
      901 gcccgagcag agcgaaggcc gcatgcgcgg ctttcagctg tgggtgaacc tgcccgccag
      961 gctaaagatg agcgcgcccg gctattcgga attcgagagc gacgccatcc cggtcgaggc
     1021 gcgcgacggc ggcgtctcgg tcaaggtgat ctctggcgcc acggagggcg gcacggccgg
     1081 cccgatcggc ggcggagcgg tggatgccct ctatttcgac gtcgcgctgc ccgccgggac
     1141 gcggttcgag gagcccgtcg gcggcgaccg caacgccatg gtcgcggtct atgagggcca
     1201 ggtccgcgtg gcgggcggca agatcgacgc gctgagcggg gtgttcctgg gcgccggcga
     1261 cagggtggtg gtcgaggcgg tcaccgacgc gcgcgtgctg ctgctggccg
//
LOCUS       DAOMEO010001062         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10332_length_1309_cov_2.697767, whole genome shotgun sequence.
ACCESSION   DAOMEO010001062 DAOMEO010000000
VERSION     DAOMEO010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_1309_cov_2.697767"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLH31_18360"
     CDS             <1..689
                     /locus_tag="PLH31_18360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR91191.1"
                     /translation="TGVKDADFAQIDLWSCAMARAGYSAVPGQAIADLSIDFTDLETY
                     LARLSSGVRRDMRRKLRSRGKVRIELRADLGKDLPRAMALYYQTLARADAQLETLTPA
                     FFKAVADRLPGRAAFALYYVEDDLLAFNLLLEDGEVLLDKFFCMEAERGRAHNLYFLS
                     WFFNLEHCLARGLKRYQSGQANLGDKLRLGSRLTPAATYFKHRNGLLNGALRMLAPLV
                     MGDPLEGLAA"
     gene            686..1105
                     /locus_tag="PLH31_18365"
     CDS             686..1105
                     /locus_tag="PLH31_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91192.1"
                     /translation="MTRELQTPPRRRFAPAILFAILPIISLAYQITAKTSANKLAGAQ
                     FDWAWLAAAARLPSVQLLVALEIAAFAAWMTVLAEMPLSAAFPLSAVSYVLIIAASAV
                     VFHEPISLLQVVGSLAILLGVLLIGRGAKTVELAADA"
     gene            1102..>1309
                     /locus_tag="PLH31_18370"
     CDS             1102..>1309
                     /locus_tag="PLH31_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR91193.1"
                     /translation="MTSIALKTLLFDWRRFAPAAVAVGFSGLLVLLQAGLILGVFSLA
                     SVCVTKSSADLWVGFPGVQSIDLGR"
BASE COUNT          186 a          443 c          420 g          260 t
ORIGIN      
        1 tgacgggcgt caaggacgcc gacttcgctc aaatcgacct gtggtcatgc gccatggcca
       61 gggccggcta cagcgccgtt ccggggcagg ccatcgccga cctctccatc gatttcaccg
      121 acctcgaaac ctatctggcg cggctgtcca gcggcgtgcg ccgcgacatg cgccgcaagt
      181 tgcggtcgcg cggcaaggtc cggatcgagc tgcgtgcgga cttgggtaag gatctgcccc
      241 gggccatggc gctctattat cagaccctgg cccgtgccga cgctcagctg gagacgctga
      301 cgccagcctt cttcaaggcc gtggccgacc gcttgccggg tagggcggcg ttcgcgctct
      361 actatgtcga ggacgacctg ctggccttca acctgctgct cgaagacggc gaggttctgc
      421 tcgacaagtt cttctgcatg gaggccgagc gcggccgcgc tcacaacctc tatttcctga
      481 gctggttctt caatctcgag cactgtctgg cgcggggcct gaagcgctat cagagcggcc
      541 aggccaatct gggcgacaag ctgcgcctgg gcagccggct gacacccgcg gcgacctatt
      601 tcaagcatcg caacggcctg ctcaacggcg cgcttcgcat gctagcgccg ttggtcatgg
      661 gcgatccgct ggaaggtctg gccgcatgac ccgggaactc cagactccgc cccgccgccg
      721 cttcgcccct gccatactgt tcgccatcct gccgatcatc agtctggcct atcagatcac
      781 cgccaagacc tcggccaaca agctggcggg cgcgcaattc gattgggcat ggctggccgc
      841 cgccgcgcgc ctgcccagcg ttcagctact ggtagcgctt gagatcgccg ccttcgcggc
      901 ctggatgacg gtcctggccg agatgccgct gagcgccgcc tttccgctgt cggcggtcag
      961 ctacgttctg atcatcgccg ccagcgcggt cgtctttcat gagcccatca gcctgctgca
     1021 ggtggtgggc agcctggcga tcctgctcgg cgtcctgctg atcgggcgcg gggccaagac
     1081 ggtcgaactg gccgccgacg catgacctcc atcgcgctta agaccctgct gttcgactgg
     1141 cgccgctttg ccccggcggc tgtggcggtg ggcttttccg ggcttctggt gctgttgcag
     1201 gcggggttga tcctgggcgt gttctcacta gccagcgtct gtgtgaccaa atcctcggcc
     1261 gatttgtggg tcgggtttcc cggtgtgcag agcatcgacc tgggtcgcc
//
LOCUS       DAOMEO010001063         1309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10336_length_1309_cov_2.462520, whole genome shotgun sequence.
ACCESSION   DAOMEO010001063 DAOMEO010000000
VERSION     DAOMEO010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10336_length_1309_cov_2.462520"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(754..>1309)
                     /gene="rnc"
                     /locus_tag="PLH31_18375"
     CDS             complement(754..>1309)
                     /gene="rnc"
                     /locus_tag="PLH31_18375"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQR91194.1"
                     /translation="EFIGDRVLGLLAAEALAERFPQAKEGELAPRLNALVSRETCARV
                     ARAAELGPALRLSASSSKIGGRETDSILAGGCEALMAALYRDGGLDAARRVFLDLWSA
                     EFDRAAQGKPRDPKTALQEWAQSQGKPLPTYAVKDRTGPDHAPVFTVQVVVAGVDPVF
                     ATGRSRQDAEKAAAKALLDREGAE"
BASE COUNT          213 a          441 c          444 g          211 t
ORIGIN      
        1 cagaccggcg atcgtctcgc cgtgagattt aaggatggct cccttcggcg cgaaggttgg
       61 gcggtgcggg acgcgctcga ttatatcctc cccctaacgg gggaggtgtc ggcgaagccg
      121 acggaggggg aagtgactgc tgactcattg cggtcttccc cctccggcct tcggccgcct
      181 cccccgctag ggggaggatc ttcgctctct atcctctgcg ttcagcgaat cgaagccagc
      241 aaaatcagtt acttccaact ttctcgccct tctgtatccc ccgccccaaa acctgatgga
      301 tactgcttgc tgtccccgtc gcagggggag ggcgcttggt accggcctga tgcggcgatg
      361 gggatggagc tgagcggggc cgcgacgccc tggctgcgga agcgtccgtg cgggctgcct
      421 cagaagggcg gaacaatctg gaagcgacca cggccgagcg agcgcgggtt gaagagggcg
      481 gggatcagaa gccgcctatc gcacatcgtc ggcgtcgaga cggattagcc gatcgacggg
      541 atgcgcgccc gtccgggggt taatcgggaa tcagagctga aactcacgct cgtgtgcccc
      601 gatcctcacg gtcaagaaca gtttctgcga agcggacagg tcgagccgaa acgcacaaaa
      661 cacatcacgg tcatccgggt tcgccccgga ccgcttcgcg cctccccatc cccttcagcg
      721 gcgaatgcca cgtgaggcta cgagcccgtg tccctactcc gccccttccc gatccagcag
      781 cgccttggcg gcggcctttt cggcgtcctg gcgcgagcgg ccggtggcga agacgggatc
      841 gacgccggcc accaccacct gcacggtgaa caccggcgca tgatcgggac cggtgcggtc
      901 cttgacggca taggtcggca ggggcttgcc ctggctttgc gcccattcct gaagcgcggt
      961 cttgggatcg cgcggcttgc cttgcgcggc gcggtcgaac tcggcgctcc acaggtccag
     1021 gaacacccgg cgcgcggcgt caaggccgcc atcgcggtag agcgccgcca tcagggcctc
     1081 gcagccgccg gccaggatcg agtcggtctc acgaccgccg atcttgctgg acgaggccga
     1141 caggcgaagg gccggaccaa gctcggcggc gcgggccacg cgggcgcagg tttcgcggct
     1201 aaccagggcg ttgaggcgcg gggccagttc gccctccttg gcctgcggaa accgctcggc
     1261 cagggcctcg gcggccagca gccccagcac gcggtcgccg atgaactcc
//
LOCUS       DAOMEO010001064         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10351_length_1308_cov_1.981644, whole genome shotgun sequence.
ACCESSION   DAOMEO010001064 DAOMEO010000000
VERSION     DAOMEO010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10351_length_1308_cov_1.981644"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLH31_18380"
     CDS             <1..1008
                     /locus_tag="PLH31_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HQR91195.1"
                     /translation="ARIVENPERLRLLLVITVADIRAVGPGVWNGWKGQLLRELYNAT
                     EAVFRGGRGSDAAASVQRHQEAAAEAARAALVADDPAAKGWAAAMEDAYFGAFKLDEL
                     KDHAALARRAAIQGGAAAEGQVTPGSNAAEIVVAAKDRRGLFADLALAISSLGGNVVG
                     ARVFTSRQGQALDVFYVQDVTGAPLGCENPRALRRLADALEAAGRGETMSFEPRRGAE
                     LSRTAAFTIAPSVVLDNDSSDDATVVEASGRDRPGLLHALAQTLASNGLSIQSAHIDG
                     YGERAVDAFYVQTAEGGKLSETRKLNTLKADLLAALEQNEAGAPNARPGLKRARASVA
                     R"
     gene            1045..>1308
                     /locus_tag="PLH31_18385"
     CDS             1045..>1308
                     /locus_tag="PLH31_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91196.1"
                     /translation="MAKDNGARKLDAAKVVLTLDHLRIRVGERFPDSGLAQVCEQLTV
                     TARTTAQRARRLSRPYLGLRFLVLAVVLAAIVAEAVLIARLDWL"
BASE COUNT          189 a          446 c          473 g          200 t
ORIGIN      
        1 gcccgtattg tggaaaaccc cgagcggctg cgcctgctgc tggtgatcac cgtggccgat
       61 atccgcgccg tcgggccggg ggtgtggaac ggctggaagg gccagctgct gcgcgagctc
      121 tacaacgcca ccgaggcggt gttccggggc gggcggggca gcgacgccgc cgccagcgtc
      181 caacgccatc aggaggccgc cgccgaggcc gcgcgggccg ccctggtcgc tgacgacccc
      241 gccgccaagg gctgggccgc cgccatggag gacgcctatt ttggggcctt caagctggac
      301 gagctgaagg atcatgcggc gcttgcccgc cgcgccgcca tccagggcgg agctgccgcc
      361 gaggggcagg tgacgcccgg ctccaacgcc gccgagatcg tggtcgccgc caaggatcgg
      421 cgcggccttt ttgctgatct ggcgctcgcc atctcgtcgc tgggcggcaa tgtggtgggc
      481 gcgcgggtct tcacctcgcg ccagggtcag gccctggatg tcttttacgt gcaggacgtc
      541 accggcgccc cgctgggctg cgagaacccc cgggctctgc gccgcctggc cgacgccctg
      601 gaagcggccg gtcgcggcga gaccatgagc ttcgagcccc ggcgcggcgc cgagttgtcg
      661 cgcacggcgg ccttcacgat cgctcccagc gttgtgctgg acaacgactc gtccgacgac
      721 gccaccgtcg tggaagcttc gggccgcgac cgtcccggtc tgctgcacgc cctggcccag
      781 accctggcca gcaacggcct gtcgatccag tccgcccata tcgacggcta tggcgagcgc
      841 gccgtggatg cgttctatgt gcagacggcc gagggcggca agctcagcga aacgcgcaag
      901 ctcaacaccc tgaaagccga tttgctggcc gcgctggagc agaacgaggc cggcgcacca
      961 aacgcgcggc cggggttgaa acgggcgcgg gccagcgtgg cgcgctagac gtctgatcca
     1021 gaacgtgggg ataagggggc ggctatggcc aaggataacg gggcgcgaaa gctggacgcc
     1081 gccaaggtcg ttctcaccct tgatcacctg cgcattcgcg tcggggaacg atttccagac
     1141 tctggcctgg cccaggtctg cgagcagttg accgtcaccg cgcggacgac ggcccaacgg
     1201 gcccggcggc tgtcacggcc ttatctgggt ctgcgctttc tggtgttggc cgtggtgctg
     1261 gccgccattg tcgccgaggc ggtgctgatc gcacgcctgg actggctg
//
LOCUS       DAOMEO010001065         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10366_length_1306_cov_3.083133, whole genome shotgun sequence.
ACCESSION   DAOMEO010001065 DAOMEO010000000
VERSION     DAOMEO010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_1306_cov_3.083133"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..345
                     /locus_tag="PLH31_18390"
     CDS             31..345
                     /locus_tag="PLH31_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HQR91197.1"
                     /translation="MATIGTFTTTTNGFVGSIRTLSLNVKAKLVRVENPSDKGPHFRI
                     FAGANVELGAAWQKTARDTDRDYLSVKLDDPSFPAPIYATLIEVEGEEGLQLIWSRPN
                     RD"
     gene            426..623
                     /locus_tag="PLH31_18395"
     CDS             426..623
                     /locus_tag="PLH31_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91198.1"
                     /translation="MTITTNLTGAKVHRSDDIAVWPDGTWAEIGDVWSGEYTFMSDDY
                     EVVPIKDHARLDALGIADEVL"
     gene            890..1054
                     /locus_tag="PLH31_18400"
     CDS             890..1054
                     /locus_tag="PLH31_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91199.1"
                     /translation="MSIDQYIEELRAELRNAVDAVERREIEAELEMALAERELIWAEL
                     EGLLSGEPPF"
BASE COUNT          250 a          440 c          401 g          215 t
ORIGIN      
        1 gcggcccgag aaaaccaagg gaatgagaca atggcgacca tcggcacctt caccaccacc
       61 accaacgggt tcgttggctc catccggacg ctctccctca acgtcaaggc caagctggtc
      121 cgcgtcgaga atccgtccga caagggcccc cacttccgca tcttcgccgg cgcgaatgtc
      181 gagctcggcg cggcctggca gaagactgcc cgggacacgg accgcgacta cctctccgtc
      241 aagctcgacg acccttcgtt cccggccccg atctacgcca ccctgatcga ggtggaaggc
      301 gaggaaggcc tccagctgat ctggtcccgg ccgaaccggg actgagcctc cgaccagagg
      361 gccccgcctt gcggcggggc cttcagcctt tttggtagcg aacggccgca gccaggagga
      421 cacacatgac catcaccaca aatctcacag gcgcaaaggt ccaccgtagt gacgacatcg
      481 cggtctggcc tgacggaaca tgggccgaga tcggcgatgt ttggagcggc gaatacacct
      541 tcatgtcgga tgactacgaa gtggttccga tcaaggatca cgctcgcctg gatgccctcg
      601 gcatcgccga cgaggtgctc tagccggctc ccacggtgtc ccagccggaa gcaggcatcg
      661 aacctgcttc cggcttgtcg ggtgcacaac cgggagcggg gatggctgct cagatcgtcc
      721 caggatgcca tggcggcccg aaggcctcaa ggacgagcgg ttcccccaaa atgctggcgc
      781 atttcggctc ccccactcgc cggctccggc ggtcgcttcg cgctccttga tccctccggc
      841 ccgccatgac cgccggtgtc gtctttcacc aacatcaagg agacgaccaa tgtcgatcga
      901 tcagtacatc gaggaactgc gcgccgagct gcggaacgcc gtcgacgccg tggagcgccg
      961 tgagatcgag gccgagctgg agatggctct cgcggagcgc gaactcatct gggccgaact
     1021 ggagggcctg ctcagcggcg agcctccctt ctagggagct ttcgccatgt cgcgccgtcc
     1081 agggcgccac gccgttcggc gacggcgctt cggagggctc gtcgttgcac aaatgcgttg
     1141 aatgctgtat atgacagcaa aggcagcaaa tacaggagat cgccatgccg cgttcaggtg
     1201 ggtcctattc cacgagcgaa ttgtcccgga agtccggcga tatcatcgcc gaggcgctgc
     1261 gccggccggt gatgatcacc cagcgcaaca agccgcgcct cgtgct
//
LOCUS       DAOMEO010001066         1306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10370_length_1306_cov_2.100719, whole genome shotgun sequence.
ACCESSION   DAOMEO010001066 DAOMEO010000000
VERSION     DAOMEO010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10370_length_1306_cov_2.100719"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLH31_18405"
     CDS             complement(<1..427)
                     /locus_tag="PLH31_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQR91200.1"
                     /translation="MSSTASRPPSPAAPDTGAAADEPLYEARRQIYPQSVQGRFRKIK
                     WILLAITLAIYYLLPFVRWDRGPDAPHQAVLIDFPARRFYFFFLEIWPQEFYYVAGLL
                     ILAALALFLMNAVAGRIWCGYLCPQTVWTDLFLAVERLIE"
     gene            complement(510..>1306)
                     /gene="ccoP"
                     /locus_tag="PLH31_18410"
     CDS             complement(510..>1306)
                     /gene="ccoP"
                     /locus_tag="PLH31_18410"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HQR91201.1"
                     /translation="ELNNPLPRWWLWTFYACIVWSVAYWLVYPAWPLISSTTNGFLGW
                     NSRTAVEEQLSDLKALRATSSARLDAASLTEIEKSPELLALARAEGRVAFADNCAPCH
                     GAGGGGAKGFPNLNDDDWLWGGSLPQIEATLLHGIRSGDDAGHQGNMPGFAGVLSRPQ
                     MSDAAEYVRSLAGLVPATDPAAQRGAPVFKANCAVCHGEDGKGKVDLGSKNLTDNIWL
                     YGGDKATIMQTIAQGRGGVMPAWGQRLPTSTIKALTVYVHTLGGGQ"
BASE COUNT          238 a          409 c          457 g          202 t
ORIGIN      
        1 cctcgatcag gcgttcgacg gcaaggaaga ggtctgtcca gacggtctgg gggcaaagat
       61 agccgcacca gatgcgccca gccactgcgt tcatgagaaa cagggccagc gccgcgagga
      121 tcagaagacc ggcgacgtaa tagaattcct gcggccagat ttccagaaag aagaaataga
      181 accggcgggc ggggaaatcg atgagcacag cctgatgcgg tgcgtccgga ccccggtccc
      241 accgcacgaa aggcagcaga tagtagatgg ccagcgtgat cgccagcagg atccacttga
      301 tcttgcggaa acggccctgg acactctggg gatagatttg ccgccgcgcc tcatagaggg
      361 gctcgtcggc cgcagcgccg gtgtcgggcg ctgcgggaga gggggggcgg gaggcggtgg
      421 aggacatggc gtttcgcggc gaaccggctc tctcacggat tgagagactg attcaccgtc
      481 caggtcggtc aacctgaacg gatcaggctc tattgacccc cgcccagcgt gtggacgtag
      541 acggtgaggg ccttgatggt ggaggtgggc aggcgctggc cccaggccgg catgaccccg
      601 ccgcgcccct gcgcgatggt ctgcatgatg gtggccttgt cgccgccata cagccagatg
      661 ttgtcggtca ggttcttgga cccgaggtcc accttcccct tgccgtcctc gccgtggcag
      721 acggcgcaat tggccttgaa caccggcgcg ccccgctgtg cggcgggatc ggtggccggc
      781 acgaggccgg cgagcgagcg gacatattcc gccgcgtccg acatctgcgg ccggctcagc
      841 acgccggcaa agcccggcat gttgccctga tggccggcat cgtcgccgga gcggatgccg
      901 tgcagcaggg tggcctcgat ctggggcagg ctgccacccc acagccagtc gtcgtcattg
      961 aggttcggga agcccttggc cccgccgcca cccgcgccgt ggcagggcgc gcaattgtcg
     1021 gcaaaggcga ccctgccttc ggcccgcgcc agtgccagaa gctccgggct cttctcgatc
     1081 tcggtcagcg aggccgcatc aagccgggcg gaggaggtgg cccgcagggc tttcaggtcg
     1141 ctgagctgct cctctacggc cgtccgcgag ttccagccaa ggaacccgtt ggtggtcgag
     1201 gagatgagcg gccaagcggg atagaccagc cagtaggcca ccgaccagac aatgcaggca
     1261 tagaaggtcc acagccacca gcgcggcagc ggattgttga gttcgc
//
LOCUS       DAOMEO010001067         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10409_length_1303_cov_2.180288, whole genome shotgun sequence.
ACCESSION   DAOMEO010001067 DAOMEO010000000
VERSION     DAOMEO010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10409_length_1303_cov_2.180288"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLH31_18415"
     CDS             complement(<1..306)
                     /locus_tag="PLH31_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR91202.1"
                     /translation="MQNLFSLEGRVAVVTGGSRGIGAMIAKGFLTYGAKRVYITARKA
                     GACDAAAEELSQYGECVSLPGDISTMEGIEGLASRIKEREDKLDILVNNAGAAWGAPF
                     "
     gene            complement(319..1254)
                     /locus_tag="PLH31_18420"
     CDS             complement(319..1254)
                     /locus_tag="PLH31_18420"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQR91203.1"
                     /translation="MMNRVLAHTGAKYPIVQAPMGWIARYPLASAVSRAGGLGIIETS
                     SGETENCKAEIRKMAETGLPFGVNLPILFLRDDSMLKFVCESGVKFVTTSAGSPAKFI
                     GPLKDAGIVVYHAVPTVDAAVKCAEAGIDGLVVEGAEGGGFKNPEEVSTLVLLQAIRA
                     RVDVPLIAAGGICDGRGMAAAFALGAEAIQMGTRFVSSVESPVHDNYKAAITGGKETG
                     TWILNKKASPCIRALKSERTKAIYDEGLMPPDALKGILGVYFGGDMEAAPALAGQTVG
                     LIDEVKTAQQIIDETVAQFHDITGRLGALAATRGF"
BASE COUNT          204 a          430 c          441 g          228 t
ORIGIN      
        1 aaagggcgcg ccccaggctg cgccggcgtt gttgaccagg atgtcgagct tgtcctcacg
       61 ttccttgatc cgcgaggcaa ggccctcaat gccctccatg gtcgagatgt cgcccggcag
      121 cgagacgcat tcgccatatt gcgacagctc ttcagcggct gcgtcgcagg cgccggcctt
      181 gcgggcggtg atgtagacgc gcttggcgcc ataggtgagg aagcccttgg cgatcatggc
      241 gccgataccg cgcgagccgc cggtgacgac cgcgacgcga ccttcgagcg aaaacagatt
      301 ttgcatggtc ttgatccttt agaagccgcg cgtggcggcc agggcgccta ggcgcccggt
      361 gatgtcgtgg aactgggcga cggtctcgtc gatgatctgc tgggccgtct tgacctcgtc
      421 gatcagaccg acggtctggc cggccagggc cggagccgcc tccatgtcac cgccgaaata
      481 gacgccgagg atgcccttca gagcgtcggg cggcatcagg ccctcatcat agatggcctt
      541 ggtgcgctcg gacttcaggg cgcggatgca ggggctggcc ttcttgttga ggatccaggt
      601 gccggtttcc ttgccgcctg tgatcgcggc cttgtagttg tcgtgcaccg ggctctcgac
      661 cgagctgacg aagcgggtgc ccatctggat cgcctcggcc cccagggcga aggcggcggc
      721 catgccgcgc ccgtcgcaga taccgccggc cgcgatcagc ggtacgtcga cccgggcgcg
      781 gatggcctgc agcaggacaa gcgtcgagac ctcttccggg ttcttgaagc cgccaccctc
      841 ggcgccctcc accaccaggc cgtcgatgcc ggcctcggcg catttgaccg ccgcatcgac
      901 ggtgggcacg gcgtgataga ccacgatgcc ggcgtccttc agcggaccga tgaacttggc
      961 cgggctgccg gccgaggtgg tgacgaactt gaccccgctc tcgcagacga acttcagcat
     1021 ggagtcgtcg cgcaggaaca ggatcggcag gttcacgccg aacggcaggc ccgtctccgc
     1081 catcttgcgg atctcggcct tgcagttttc ggtctcgccc gacgaggtct cgatgatgcc
     1141 cagaccgccg gcccgcgaca ccgccgaggc cagcggatag cgggcgatcc agcccatcgg
     1201 ggcctggacg atggggtact tggcgcccgt atgggccaga acgcggttca tgatcatctc
     1261 gctcatggga tcagcagcac gcggccaacg gcctggcggc ttt
//
LOCUS       DAOMEO010001068         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10410_length_1303_cov_2.100962, whole genome shotgun sequence.
ACCESSION   DAOMEO010001068 DAOMEO010000000
VERSION     DAOMEO010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10410_length_1303_cov_2.100962"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLH31_18425"
     CDS             complement(<1..451)
                     /locus_tag="PLH31_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQR91204.1"
                     /translation="MIARIQSTYKTLGPTSRLIADFILSQPDAVMRMSVTELSEQTGS
                     SQGSVVNFCQNLGLSGFQQLKLSLAQAVVQPVQYIQEDLARTDDVETICRKMFHAGIQ
                     ALRDSMSVLDPKSVAAAVEAIRKAKRIEIYGIGSSAPIAEDAHYRMLR"
     gene            856..>1303
                     /locus_tag="PLH31_18430"
     CDS             856..>1303
                     /locus_tag="PLH31_18430"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HQR91205.1"
                     /translation="MILIPADGGTEAGTKADYQPLFNAISRMSGLRFDIRVGQSYGAV
                     VEALCNGTADIAFVGPVTYIQANKRRCAELLAVAVEKGQSIYYAGLFTRAGSPITSVK
                     GLKGKRVAFGDVNSTSSFVYPMAMVLDAGLDPVRELNAVRMTGSHGN"
BASE COUNT          252 a          385 c          397 g          269 t
ORIGIN      
        1 tgcggagcat ccggtagtgg gcgtcttcgg cgatcggggc cgaagatccg atgccataga
       61 tttctattcg cttggccttg cggatcgcct caacggctgc ggcgacggac ttgggatcaa
      121 gtacgctcat cgagtctcgc agggcctgga tcccggcgtg gaacatcttg cggcagatcg
      181 tctcgacatc gtcggttctg gccagatcct cctgaatata ctgcactggc tgtacgacag
      241 cctgggcaag cgagagcttc agctgttgaa accctgaaag tccaaggttc tggcagaagt
      301 tgaccacgct gccctggctc gatccggtct gttcggagag ctccgtaacc gacatccgca
      361 tgacggcgtc cggctgactt aatatgaagt cggcgatcag ccgggaggtg ggccccaggg
      421 ttttgtaagt cgactggata cgggcgatca cgttgtggac cggcgtatgg ccggtgcttc
      481 gcggccgcga cttcgctgga ttaggccttt ggtcggacac ggatgcctgc tttcaaccaa
      541 gtaggggtca catcgatagg atggctacat cgcgcggcga gagtattcga cctaggcgag
      601 tcgccccggt ccgtcttgca cgggatccat atcaaaagat ccgatcacta tgactcccgg
      661 aaggacgggt atgaaatata ctcattctca gaaatttcaa atctgagaaa gcttcacggg
      721 ggcgtcacaa gcgtgcgtta aggctgtgac cggctcagcc ggccctggag tactagcatg
      781 cgacgcttga tattggtgct ccttctgttg gccctggcgc ccaccgcagc cacggcaacc
      841 caacggccgc tgaacgtgat cctgatcccc gccgacggcg gtaccgaagc cggcacaaag
      901 gccgactatc agccgctgtt caacgccatc tcgcgcatga gcggactgcg attcgatata
      961 cgggtgggcc aatcctacgg cgctgtagtc gaggccctct gcaacggcac agccgacatc
     1021 gcgttcgtgg gaccggtgac ctatatccag gccaacaagc gtcggtgcgc cgaactgctg
     1081 gccgtcgcgg tcgagaaggg tcagtcgatc tactatgccg gcctgttcac ccgggccggt
     1141 tccccgatca cctctgtcaa gggcctcaaa ggcaagcgtg tcgcgttcgg cgatgtgaac
     1201 tctacctcat ccttcgtcta cccgatggcg atggtcctgg atgcggggct tgatcctgtc
     1261 cgcgagctca acgccgttcg tatgacggga agccacggca acg
//
LOCUS       DAOMEO010001069         1301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10423_length_1301_cov_2.708668, whole genome shotgun sequence.
ACCESSION   DAOMEO010001069 DAOMEO010000000
VERSION     DAOMEO010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10423_length_1301_cov_2.708668"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLH31_18435"
     CDS             complement(<1..237)
                     /locus_tag="PLH31_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91206.1"
                     /translation="MSAPHLLVHEKKDTVGVVVVEGLTAGTDMLCVVTHDNSAFNLTS
                     KMDVPIGHKVALTDIKAGDTIWKYGQDIGVAKADI"
     gene            427..>1301
                     /locus_tag="PLH31_18440"
     CDS             427..>1301
                     /locus_tag="PLH31_18440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HQR91207.1"
                     /translation="MARLSKWIAAGIAGLALSVGGAVAQSFPTRPITVIVPYSAGGPS
                     DAIARLLGQNMSATLGQPIVIENVVGAGGTTGAGRLAKASPDGYTLLIHHVALAASAA
                     LYTNLTYDTNKAFETVGLVNYGPMILITKKDYAANNIAELIAKVKADGAKTTIAHAGV
                     GSNSYLCAQLIQKALDVKMTDVGYRGTGPAMNDLMGGQVDMQCEQSTTAVPQVKGNTV
                     KGFAVTSAKRLDVVKDLPTLQEAGLKGFDFVIWHGIYAPKGTPADVVAKLNAALQKAL
                     NDPAIQQKFTDVGTSV"
BASE COUNT          257 a          407 c          399 g          238 t
ORIGIN      
        1 gatgtccgcc ttcgcgacgc cgatgtcctg gccgtacttc cagatcgtgt cgcccgcctt
       61 gatgtccgtg agcgcgacct tgtggccgat cggcacgtcc atcttggaag tgaggttgaa
      121 ggcggaattg tcgtgggtga caacgcacag catatccgtg ccggccgtca ggccttccac
      181 caccacgact ccaaccgtat cttttttctc gtgaaccaac aggtgcggcg cactcacgtt
      241 tgcctcctcg cctcttgcga cttgccgttt gcgatcaagt cttctataag acgtgagata
      301 gatgagttaa acggatggcg gtgtcaagcg gagttccgca cccattgtcc aaaactcacg
      361 aaaaatgccg atgacccaaa ggcttaagcg ggcatcgcag aggaaacgcc aaacaggagg
      421 ttgccgatgg cgcgtttgtc gaagtggatt gcagcgggca ttgcagggct cgctctgagc
      481 gtcgggggcg cggttgcgca gtccttcccg acacggccga tcacggtgat cgtgccctat
      541 tccgcaggcg gacccagcga tgccattgcg cgtctgctcg ggcagaacat gtcggccacg
      601 ctgggccagc ccatcgtgat cgagaatgtg gtcggtgccg gcggcacgac gggcgccgga
      661 cgcctcgcga aggcctcgcc ggacggctac acgctgctga tccatcacgt cgccctcgcg
      721 gcgagcgcgg cgctctacac caacctcacc tacgacacca acaaggcgtt cgagacggtg
      781 ggcctggtca attacggccc gatgatcctc atcaccaaga aggattacgc cgccaacaac
      841 atcgccgagc tgatcgccaa ggtgaaggcg gacggcgcca agaccaccat tgcccatgcg
      901 ggtgtcggct cgaattccta tctctgcgcc cagctgatcc agaaggcgct ggatgtgaag
      961 atgaccgatg tcggctatcg cggcacgggt ccggccatga acgacctgat gggcggtcag
     1021 gtggatatgc agtgcgagca gtcgaccacc gcggttccgc aggtcaaggg caacaccgtt
     1081 aagggctttg ccgtcacctc ggccaagcgg ctcgacgtgg tgaaggacct tccgacgctt
     1141 caggaagccg gcctgaaggg cttcgacttc gtgatctggc acggcatcta cgcgcccaag
     1201 gggaccccgg ccgacgtggt ggccaagctc aatgccgctc tccagaaggc gctgaacgat
     1261 cccgccatcc agcagaagtt caccgatgtg ggcaccagcg t
//
LOCUS       DAOMEO010001070         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10429_length_1300_cov_2.946185, whole genome shotgun sequence.
ACCESSION   DAOMEO010001070 DAOMEO010000000
VERSION     DAOMEO010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10429_length_1300_cov_2.946185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1300
                     /locus_tag="PLH31_18445"
     CDS             <1..>1300
                     /locus_tag="PLH31_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91208.1"
                     /translation="GDSTAAEALTRVSGLSLVSGRFVYVRGLGERYSSALLNGSPLPS
                     PEPLQRVVPLDLFPSSILAGATVQKSYSPNYPGEFGGGLIDLQTIAVPDKAFFSFEIG
                     TGGNTETTLNKGLTYYGSDTDFLGYDDGTRKKPPELRAALATGKRISSGNFTTAELTR
                     VGQSLVNAPLNVIQVKDKIPLNFSTDISAGQSYDLGWGHLGVVAIGGFENSWRTRQGK
                     QQEGLEQNGVLETRTDYDFMATSNNVVVNGLFGLGLEWGENQIKWTNLYIHSTTKSAR
                     QRQGFDELAGQQVLDSYTEWFERSLLDTQIAGKHEFGAWNFDWRGSYAKSERDAPYEK
                     FIRYREDNGVYYHSASQEQNSTSFSELDDTVASAGFDVQYTLPLSSGRDAIFSGGYAY
                     MDNQRDAERRDFRFQGGAIPATVQQERVDFLLSDYNIAQGF"
BASE COUNT          250 a          436 c          390 g          224 t
ORIGIN      
        1 gggtgacagc accgccgccg aagccctgac gcgcgtcagc ggcctgagcc tggtgtccgg
       61 ccggtttgtc tatgttcgcg gcctgggcga gcgctactcg tcggccctgc tgaacggctc
      121 gccgctgccc agccccgagc cgctgcagcg cgttgttccg cttgacctgt tcccgtccag
      181 catcctggct ggcgcaacgg tccagaagag ctactcgccc aactatccgg gcgaattcgg
      241 cggcggtctc atcgacctgc agacgatcgc cgtgccggac aaggccttct tcagctttga
      301 gatcggcact ggcggcaaca ccgagacgac cctgaacaag ggcctgacct actacggttc
      361 ggacaccgac ttcctgggtt atgacgacgg cacccgcaag aagcctccag aactgcgcgc
      421 ggcgctggcc accggcaagc gtatcagctc gggcaacttc accacggccg aactgacccg
      481 cgtcggtcag agcctggtca atgcgccgct gaacgtgatc caggtcaaag acaagatccc
      541 cctgaacttc tccaccgaca tcagcgccgg ccagtcctat gatctgggct ggggccacct
      601 gggcgtggtc gccatcggcg gcttcgaaaa cagctggcgc acgcgtcagg gcaagcagca
      661 ggaaggcctg gaacagaacg gcgttcttga gactcgcacc gactacgact tcatggccac
      721 cagcaacaac gtggtggtga acggtctgtt cggtctgggc cttgaatggg gtgaaaacca
      781 gatcaagtgg accaacctct acatccacag caccaccaag tcggcgcgtc agcgccaggg
      841 ctttgacgag ctggccgggc agcaggttct ggactcctac accgagtggt tcgagcgctc
      901 gctgctcgac acgcagatcg ccggcaagca cgagtttggc gcctggaact ttgactggcg
      961 cggttcatac gccaagtccg agcgcgacgc gccgtacgag aagttcatcc gctatcgcga
     1021 agacaatggc gtttactacc acagcgcctc gcaggagcag aactcgacca gcttcagcga
     1081 actggacgac accgtcgcca gcgccggctt cgacgttcag tacaccctgc cgctgtcctc
     1141 gggccgggac gccatcttca gcggcggcta cgcctatatg gacaaccagc gcgacgctga
     1201 acgtcgtgac ttccgcttcc agggcggtgc cattccggcg actgttcagc aggagcgtgt
     1261 cgacttcctg ctgtcggact acaacatcgc ccagggcttc
//
LOCUS       DAOMEO010001071         1300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10432_length_1300_cov_2.340562, whole genome shotgun sequence.
ACCESSION   DAOMEO010001071 DAOMEO010000000
VERSION     DAOMEO010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_1300_cov_2.340562"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..295)
                     /gene="trbK-alt"
                     /locus_tag="PLH31_18450"
     CDS             complement(32..295)
                     /gene="trbK-alt"
                     /locus_tag="PLH31_18450"
                     /inference="COORDINATES: protein motif:HMM:NF039468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative entry exclusion protein TrbK-alt"
                     /protein_id="HQR91209.1"
                     /translation="MKPPRPGRALLVLGLGVVVVALLLWLSPKATGNAGPSAQPAPSG
                     KADPLRADLLRCNELGAAALDDAACKEAWAENRRRFLGAREAR"
     gene            complement(332..1075)
                     /gene="trbJ"
                     /locus_tag="PLH31_18455"
     CDS             complement(332..1075)
                     /gene="trbJ"
                     /locus_tag="PLH31_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HQR91210.1"
                     /translation="MNARLRSLLAAGAAAVTLSALTVPAQAQITVWDPTNYTQNVLQA
                     ARSLQQVTNQITSLQNEAQSLINQARNLTSLPHSSLQQLQQSVARTQQLLNEAQGIAY
                     DVQQIDRAFQTSYAPARASSSDQALFASARQRWETSVKALQDAMRVQAGVVGNIETNR
                     TQMSALVGASQSATGALQAAQAGNQLLALQAQQLADLTAAVAAQGRAQSLEAAQRAAA
                     ADQGREQLRRFLTPGPGYQPTDVTMFHDR"
     gene            complement(1072..>1300)
                     /locus_tag="PLH31_18460"
     CDS             complement(1072..>1300)
                     /locus_tag="PLH31_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91211.1"
                     /translation="KRDYYCQSRRGNRLFELGLGEVALAFTAASSKADQGLISRLMAE
                     QGAPGFAKAWLEARGAAWAADLIHPQQETMP"
BASE COUNT          181 a          393 c          515 g          211 t
ORIGIN      
        1 aggaagcggt cgatgacgcc cgtgccttcc atcagcgggc ctcccgcgcg ccaaggaagc
       61 gacgacggtt ctcagcccaa gcttccttgc aggccgcgtc atcgagtgcg gcggcgccca
      121 gctcgttgca gcggaggagg tcggcgcgga gcggatctgc cttgccggag ggcgcgggct
      181 gagcgctcgg tccggcgttg ccagtcgcct ttggggagag ccatagaagc agcgcaacga
      241 ccacgacgcc gaggccgagc acgagaaggg ctcggcccgg cctcggtggc ttcatcccaa
      301 tcgcgaacgg gcgggagagg cgcatgatgc gtcagcgatc gtggaacatg gtgacgtcgg
      361 tcggctgata gccggggccg ggggtcagga agcggcgaag ctgctcgcgg ccctgatcgg
      421 cggcggcggc ccgttgggcg gcttcgagac tttgggcgcg gccttgggcg gcgacggcgg
      481 ccgtcaggtc ggccagctgc tgggcctgca acgcgaggag ctggttgccg gcctgggcgg
      541 cctgcagcgc tccggtcgcc gactggctgg cgcccaccag ggccgacatc tgggtgcggt
      601 tggtctcgat gttgccgacg acgccggcct gcacgcgcat ggcgtcctgc agcgccttca
      661 ccgaggtttc ccagcgctgg cgggccgagg cgaaaagcgc ctggtcggag ctggaggccc
      721 gggccggcgc gtagctcgtc tgaaacgccc ggtcgatctg ctggacgtcg taggcgatgc
      781 cttgcgcctc attcagaagc tgctgggtgc gggccaccga ttgctggagc tgttgcagcg
      841 aggaatgggg caggctggtc aggttgcggg cctggttgat caggctttgc gcctcgttct
      901 gcagcgaggt gatctggttg gtgacctgct ggagcgagcg cgccgcctgc aggacgttct
      961 gggtgtagtt ggtcggatcc cagacggtga tctgggcctg ggcgggaacg gttagcgccg
     1021 agagggtcac ggccgcggcg ccggcggcca ggagggagcg aaggcgggca ttcatggcat
     1081 ggtctcctgc tgggggtgga tcagatctgc ggcccaggcg gcgccgcgcg cttcgagcca
     1141 ggccttggcg aagcctgggg cgccctgttc ggccatgagg cggctgatca ggccttgatc
     1201 ggccttgctg gacgcggcgg tgaaggcgag cgcaacctcg cccaagccca gttcgaacag
     1261 gcgattgccg cggcgcgact ggcagtaata gtcgcgcttg
//
LOCUS       DAOMEO010001072         1298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10450_length_1298_cov_2.555913, whole genome shotgun sequence.
ACCESSION   DAOMEO010001072 DAOMEO010000000
VERSION     DAOMEO010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10450_length_1298_cov_2.555913"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLH31_18465"
     CDS             <1..495
                     /locus_tag="PLH31_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR91212.1"
                     /translation="ATLHRRQHGGGQERGRRAGTENVAGIAAFGAAADAAVRDLASMV
                     ELGLWRDALAERVKAEGAVVLGEGADRLPQTLCFAGEGFGSEIQVMNLDLAGVMASAG
                     SACSSGKVKASRVVEAMGRPDLAPYALRVSGGWASTQDDWIKCGDAWIAAWKRIDARR
                     REMA"
     gene            495..>612
                     /locus_tag="PLH31_18470"
     CDS             495..>612
                     /locus_tag="PLH31_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91213.1"
                     /translation="MAAVQETVEAVAALEKYEHGFTTDIDQEFAPKGLSEDIV"
     assembly_gap    613..622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <623..>1298
                     /locus_tag="PLH31_18475"
     CDS             <623..>1298
                     /locus_tag="PLH31_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQR91214.1"
                     /translation="AYRRWLELEEPDWAKVNYPKIDYQDSYYYAAPKTKEGPKSLDEV
                     DPEILAVYAKLGIPLREQEVLAGVEGAPRYAVDAVFDSVSVVTTFKKELAAVGVIFCS
                     MSEAIREHGDLVKQYLGSVVPTSDNYFACLNSAVFSDGSFVYIPPGVRCPMELSTYFR
                     INAKDSGQFERTLIIADKGAYCSYLEGCTAPMRDENQLHAAVVELVLLDDAEIKYSTV
                     QNWYPGD"
BASE COUNT          231 a          413 c          428 g          216 t
ORIGIN      
        1 gccaccctgc atcgccgtca gcacggcggc ggtcaggagc gcggtcgccg ggccggcacc
       61 gagaacgtcg cgggcatcgc ggccttcggc gcggcggccg atgcggccgt ccgcgacctt
      121 gcctcaatgg tcgaactggg cctttggcgc gacgccctgg ccgaacgcgt caaggcggaa
      181 ggcgcggtcg tgctgggcga gggcgcggat cgcctgcctc aaaccctgtg ctttgctggc
      241 gaaggctttg gctccgagat ccaggtgatg aaccttgatc tggcgggcgt gatggccagc
      301 gccggcagcg cgtgctcgtc gggcaaggtc aaggccagtc gcgtggtgga agccatgggc
      361 cgtccggatc tggctcccta cgccctgcgg gtcagcggtg gctgggccag cacgcaagac
      421 gactggatca aatgcggcga cgcctggatc gccgcctgga agcgtatcga cgcacggcgt
      481 cgggagatgg cgtaatggcc gcagttcaag aaaccgtcga agccgtcgcc gcgctcgaaa
      541 agtacgagca cggcttcacc accgatatcg atcaggagtt cgcccccaag ggcctctccg
      601 aagacatcgt gcnnnnnnnn nncgcctatc gccgctggct tgagctggaa gagcccgatt
      661 gggccaaggt caactatccc aagatcgact accaggactc ctattactac gccgccccca
      721 agaccaagga gggccccaag agcctcgacg aggtcgatcc cgagatcctg gccgtctatg
      781 ccaagctcgg catcccgctg cgcgaacagg aggttctggc cggcgtcgaa ggagcccctc
      841 gatacgccgt ggacgcggtg ttcgacagcg tcagcgtggt gaccaccttc aagaaggagc
      901 tggcggcggt cggggtgatc ttctgctcga tgagcgaggc gatccgcgag cacggcgatc
      961 tggtgaagca atatctgggc tcggtagtgc cgacctcgga caactatttc gcctgcctca
     1021 acagcgcggt cttttctgac ggctcgttcg tctacatccc gccgggcgtg cgctgcccga
     1081 tggagctgtc gacttatttc cggatcaacg ccaaggacag cggccagttc gagcgcaccc
     1141 tgatcatcgc cgacaagggc gcctattgct cttacctcga gggctgcacg gccccgatgc
     1201 gcgacgagaa ccagctgcac gccgccgtgg tcgaactggt cctgctggac gacgccgaga
     1261 tcaaatattc gacggtgcag aactggtatc cgggcgat
//
LOCUS       DAOMEO010001073         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10455_length_1297_cov_2.847826, whole genome shotgun sequence.
ACCESSION   DAOMEO010001073 DAOMEO010000000
VERSION     DAOMEO010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10455_length_1297_cov_2.847826"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..400)
                     /locus_tag="PLH31_18480"
     CDS             complement(29..400)
                     /locus_tag="PLH31_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HQR91215.1"
                     /translation="MTLGETSALDLAARRLERAVAQLEQRLAARAAVAVASATASSAS
                     SSDLFDGEAARLKAELDAARERERALEDAGAQASAALGRAIAEIRAALGDEGPVNDDH
                     PEDDCDLSDETDELFDDPREA"
     gene            620..>1297
                     /locus_tag="PLH31_18485"
     CDS             620..>1297
                     /locus_tag="PLH31_18485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQR91216.1"
                     /translation="MALRVAINGFGRIGRLVLRSIVEHARRDIEVVAINDLGPVETNA
                     HLLRYDSVHGRFPGVVTSGEDWIDVGLGKIKVTAIRDPAQLPHKDLNVDIAFECTGLF
                     TTKEKASAHLTAGAKRVLVSAPCDGADQTIVFKVNDDVLSAADLVVSNGSCTTNCLAP
                     IAKVIHDLVGIERGYMTTIHSYTGDQPTLDTMHKDLYRGRAAALSMIPTSTGAAKALG
                     LVLPALKG"
BASE COUNT          223 a          469 c          403 g          202 t
ORIGIN      
        1 tcacctgaat ggtcagttgc gccatgggtc aggcctcccg cggatcatcg aacagctcgt
       61 cggtctcgtc ggagagatcg cagtcgtcct cgggatgatc gtcattgacc ggtccctcat
      121 cgccaagcgc cgcgcggatc tcggcgatag cgcgaccgag ggccgccgag gcctgggcgc
      181 cggcgtcctc cagagcccgc tcgcgctcgc gggcggcgtc cagctcggcc ttcaatcgcg
      241 cggcctcgcc gtcgaacagg tcggacgacg atgcagacga cgccgtcgcc gaagcgaccg
      301 caacggccgc ccgcgcggcc agacgctgct caagctgagc cacggcgcgc tccagtcgac
      361 gcgcggcgag gtcgagggcg ctcgtttcgc ccagggtcat cggcgggttc caatcatgcg
      421 gtgcaacata tagatcaggg ccagcttcgc cacccgctgc ggcgacaata gggtgatgaa
      481 atcgccttgg cagtaatccg acaggcattg ttaccggcgc cgggcttgac ttccccgtcc
      541 ctgcccgcaa aacgcgcctg caaaatcagg gaacgttggt ccctctgggc cgcgtccgat
      601 cgttaggaga aagaccacca tggctcttcg cgtcgccatc aacggctttg gccgtatcgg
      661 ccgccttgtc ctgcgctcca tcgtcgagca cgcgcgtcgt gacatcgagg tcgtggcgat
      721 caacgatctg ggcccggtcg agaccaacgc ccacctgctg cgctacgaca gcgtccacgg
      781 ccggttcccg ggcgtcgtga cctcgggcga ggactggatc gatgtgggcc tgggcaagat
      841 caaggtcacc gcgatccgcg atccggccca gctgccgcac aaggacctga acgtcgacat
      901 cgcgttcgag tgcacgggcc tgttcaccac caaggaaaag gccagcgccc acctgaccgc
      961 cggcgccaag cgcgtgctgg tcagcgcccc ctgcgacggc gccgaccaga ccatcgtgtt
     1021 caaggtcaat gacgacgtgc tgagcgccgc cgacctggtg gtctccaacg gctcgtgcac
     1081 caccaactgc ctcgccccca tcgccaaggt catccacgac ctggtcggca tcgagcgcgg
     1141 ctacatgacc accatccaca gctacaccgg cgaccagccg acgctggata cgatgcacaa
     1201 ggatctgtac cgcggccgcg ccgccgccct gtcgatgatc cccacctcga ccggcgccgc
     1261 caaggccctg ggcctcgttt tgccggccct gaagggc
//
LOCUS       DAOMEO010001074         1297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10458_length_1297_cov_2.494364, whole genome shotgun sequence.
ACCESSION   DAOMEO010001074 DAOMEO010000000
VERSION     DAOMEO010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10458_length_1297_cov_2.494364"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLH31_18490"
     CDS             <1..140
                     /locus_tag="PLH31_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein MotB"
                     /protein_id="HQR91217.1"
                     /translation="NDDRIYQVSGKANSEPLYADDPTLAGNRRISIVLLREKPVLPDG
                     L"
     gene            242..1084
                     /locus_tag="PLH31_18495"
     CDS             242..1084
                     /locus_tag="PLH31_18495"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HQR91218.1"
                     /translation="MTQHFPTRQLRFFLTAPTPCPYLPGREERKVFAHLPLSDGPTVN
                     DSLTQVGFRRSQNIAYRPACETCRACQSARAPAGDYIFSRSERKVLSRNGDLERHLVE
                     SEATLEQFELLRRYLLTRHADGGMAEMTWPDYVAMVEDTAVRTHLIEYRSRSQDRGPG
                     DLIACVLVDVLADGLSLVYSFYDPSLVKRSLGSFIILDHMVQAQQTGLPYVYLGYWVP
                     GSEKMAYKARFSPLEILKPGGWALMSARERGARTPRALATPREADDGELDMSDAQPAI
                     LQDL"
     gene            complement(1087..>1297)
                     /locus_tag="PLH31_18500"
     CDS             complement(1087..>1297)
                     /locus_tag="PLH31_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR91219.1"
                     /translation="VSAIAPGAFASDMNKVARDHADAVAKAIPAGRIGSEEDMAGAAI
                     YLASRAGDYVMGGAVTVDGGVSFAR"
BASE COUNT          215 a          449 c          399 g          234 t
ORIGIN      
        1 tcaatgacga ccgcatctat caggtttcgg gcaaggccaa ttccgagcca ctctatgccg
       61 acgaccccac tctggcgggt aaccgacgga tctcgatcgt tctgctgcgt gaaaagccgg
      121 tcttgcccga cggtctgtga ccgtatgccg cgccaggctt cagcgccgct tgcgcaaatg
      181 accggcatgc cgcctaatca cgtgatggcg agaggccgat aagaagggga cgggcgcttc
      241 cgtgacgcag cattttccga cgcgacagct tcggttcttc ctcacggcgc cgaccccttg
      301 cccctatttg ccgggccggg aggagcgaaa ggtctttgct catcttccat tgtccgacgg
      361 tcccacggtc aatgacagcc tgacccaggt cgggttccgt cgctcccaga acatcgccta
      421 tcggcccgcc tgcgaaacct gccgcgcctg ccagtcggcc cgcgcgccgg cgggcgacta
      481 catcttttcg cgctctgaac gcaaggtgct gtcccgtaat ggtgacctgg agcggcacct
      541 ggtcgagtcc gaggccacgc tcgagcagtt cgagttactg cgccgctatc tgctgacccg
      601 tcacgccgac ggcggcatgg ccgaaatgac ctggcccgac tatgtggcca tggtcgagga
      661 cacggcggtc cgcacccatc tgatcgaata tcgcagccgc tcacaggatc gcggccccgg
      721 cgacctgatc gcctgcgtgc tggtcgacgt gctcgctgac ggcctgtcgc tggtctacag
      781 cttctatgac cctagtctgg tgaagcgcag cctgggctcg ttcatcatcc tggaccatat
      841 ggttcaggct cagcagaccg gcctgcccta tgtctatctg ggctattggg tgccaggcag
      901 cgagaagatg gcctacaagg cccgcttttc gccgctggaa atcctcaagc ccggcggctg
      961 ggccctgatg tcagcccgtg agcgcggcgc tcgtacgccc cgcgccctgg ccaccccacg
     1021 cgaggccgac gacggcgagc tggacatgag cgacgcccag cccgccatcc tgcaggacct
     1081 atagcgctac cgcgcgaaac tgaccccgcc gtccacggtg acggcgccgc ccatgacata
     1141 gtctccggcg cgcgaggcca gatagatagc cgcgccggcc atgtcttctt ccgagccgat
     1201 acggccggcg ggaatggcct tggccaccgc gtcggcatgg tcgcgggcca ccttgttcat
     1261 gtccgaggcg aaggcgccgg gcgcgatggc gctgacc
//
LOCUS       DAOMEO010001075         1296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10469_length_1296_cov_2.120870, whole genome shotgun sequence.
ACCESSION   DAOMEO010001075 DAOMEO010000000
VERSION     DAOMEO010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10469_length_1296_cov_2.120870"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLH31_18505"
     CDS             complement(<1..188)
                     /locus_tag="PLH31_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91220.1"
                     /translation="MSGIDDPQATQAGLAQAETPVVEPVRGPAPAVTVAPAKTGIVSL
                     GLEWWPVVAFVASAAMLAT"
     gene            complement(188..793)
                     /locus_tag="PLH31_18510"
     CDS             complement(188..793)
                     /locus_tag="PLH31_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HQR91221.1"
                     /translation="MLRRLYDWVMGLAASRHAPKSLFAISFAESSFFPIPPDVMLAPM
                     VLARPDRAWHYATICTIASVLGGILGYAIGFFLQDLGMWMMAATGHAGGLAEFQCWYG
                     KYGVWVILAKGLTPIPYKLVTIASGLAAFSFPMFIVASVITRGARFFLVAWVVKKFGP
                     ALLPVIERRLALFAGGLFVLLVLGLMASHFLGGSSGGACAV"
     gene            1015..1269
                     /locus_tag="PLH31_18515"
     CDS             1015..1269
                     /locus_tag="PLH31_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HQR91222.1"
                     /translation="MSDTPPDRLATNPDSPFYNAELLERGVGIRFKGVEKTNVDEYCV
                     SEGWVRVTAGKTMDRKGNPMTIKLQGEVVPYFRDIHDAES"
BASE COUNT          264 a          426 c          419 g          187 t
ORIGIN      
        1 gtagccagca tggcggcgct ggcgacaaaa gcgacgaccg gccaccactc caggccgagg
       61 gagacgatcc cggtcttggc cggagcgacg gtcacagcag gagccggacc gcgtacgggc
      121 tcgacgaccg gcgtttcagc ctgagccaag cccgcctggg tcgcctgggg atcgtcgata
      181 ccgctcatca taccgcgcaa gcgcctccgc tgcttccacc caggaaatgg ctcgccatga
      241 ggccaagtac aaggagcacg aagaggccgc cggcgaacag ggcgaggcgc cgctcgatca
      301 cgggcagcag tgcgggacca aacttcttga ccacccaggc gaccaggaag aaccgcgcgc
      361 cgcgtgtaat cactgaagcg acgatgaaca tcggaaagct gaaggcggcc agacccgagg
      421 cgatggtcac cagcttgtag gggatggggg tcaggccctt ggccaggatc acccagacgc
      481 cgtacttgcc ataccagcac tggaactcgg ccaagccccc ggcatggccg gtggcggcca
      541 tcatccacat gcccaggtcc tgcaggaaga agccgatcgc atagcccagt atgccgccca
      601 gcacggaggc gatcgtacag atcgtggcgt agtgccaggc tcggtccggc cgcgcgagca
      661 ccatgggcgc cagcatcaca tccgggggaa tggggaagaa ggagctctcg gcgaacgaga
      721 tggcgaaaag gctcttgggg gcgtggcgcg aggcggccaa tcccatgacc cagtcgtaca
      781 ggcgacgcag catgcgagct ccaggctagt tgatgcgcgt tgctagcagg cgaagcggaa
      841 cttgtccccg gcgatgatcg gcggcgccgt ttccagtccg cgccttcacg cgttttgcct
      901 tcaccctcgg ctgccggcga gggaacgcga cgtcgcggcg cttggttttc ccgcgacgga
      961 tggctatcac cccgtccaag ggctcccgcc caccgaaatt cgaaagccga tccgatgagc
     1021 gacacccctc ccgaccgcct ggccaccaat cccgacagcc ccttctataa cgccgagctt
     1081 ctcgaacgcg gcgtcggcat ccgcttcaag ggtgtcgaga agaccaatgt cgacgaatac
     1141 tgcgtcagcg aaggctgggt gcgggtgacc gccggcaaga cgatggatcg caagggcaac
     1201 ccgatgacca tcaagctgca gggcgaggtg gttccctact tccgcgacat ccacgacgct
     1261 gagtcctgat cgtggcgctg cgtcgcctta tcgatc
//
LOCUS       DAOMEO010001076         1294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10484_length_1294_cov_2.963680, whole genome shotgun sequence.
ACCESSION   DAOMEO010001076 DAOMEO010000000
VERSION     DAOMEO010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10484_length_1294_cov_2.963680"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLH31_18520"
     CDS             complement(<1..491)
                     /locus_tag="PLH31_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQR91223.1"
                     /translation="MTGLLSLTTFAPLAGVAVLLAVRAIRGDNAATDNLAKWITLATT
                     LVTFALSIVMVAQFDAANPGFQFVEDFAWFAGLHYRMGVDGISILFVLLTAFLLPICI
                     LASWTSIDKRIVEYMICFLVLETLVIGVFCALDLILFYLFFEGGLVPMFLIIGIWGGK
                     RRV"
     gene            complement(502..>1294)
                     /locus_tag="PLH31_18525"
     CDS             complement(502..>1294)
                     /locus_tag="PLH31_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQR91224.1"
                     /translation="AAFAAGGENSIAMFAFVIGLLVAGLTAFYSWRLAFFTFNGHARW
                     GHDDHGHDAHDDHAAHASDDHGHDDHGHGHDHKPHESPLIMLFPLVVLSIGAVLAGFV
                     FVDYFVGHHQEEFWRGAIYNAPTNHVLHDAHEVPTWVKWAPLVASVLGLLGAFYVYIL
                     KEGLGAKLAARNGPLYVFFYNKWFFDELYQATFVRFAKFLGDLFWKGGDQKIIDGFGP
                     DGVSAVSYEVGRRTGKLQTGYVYHYAFVMLLGVAGLLGFALYAFR"
BASE COUNT          290 a          399 c          416 g          189 t
ORIGIN      
        1 tagacgcgac gcttgccgcc ccagatcccg atgatcagga acatcggcac caggccgcct
       61 tcgaagaaca ggtagaagag gatcaggtcc agggcgcaga acacgccgat gaccagggtc
      121 tccagcacca ggaagcagat catgtactcc acgatccgct tgtcgatcga cgtccagctg
      181 gccaggatac agatcggcag caggaacgcc gtcagcagga caaacaggat cgagatcccg
      241 tccacgccca tgcggtagtg caggcccgca aaccaggcga agtcttccac gaactggaag
      301 ccggggtttg ccgcgtcaaa ctgcgcgacc atcacgatcg acagcgcgaa ggtcaccagg
      361 gtggtcgcca gggtgatcca cttggccaga ttgtcggtgg ccgcgttgtc gcccctgatc
      421 gcgcgaaccg ccagaaggac ggccacgcca gcgagcgggg cgaaggtggt gaggctgaga
      481 aggccggtca tcgatccgtc ctcaacggaa ggcgtaaaga gcgaagccaa gcaggccggc
      541 gacgccgagc agcatgacaa acgcatagtg atagacataa ccggtctgga gcttgccggt
      601 ccggcggccg acctcgtagc tcacggcgct gacgccgtct ggcccgaagc cgtcgatgat
      661 cttctggtcc ccgcccttcc agaacaggtc gccgaggaac ttggcgaacc gcacgaaagt
      721 ggcctgatac agctcgtcga agaaccactt gttatagaag aacacgtaca gcgggccatt
      781 gcgggccgcg agcttggcgc ccaggccttc cttcaggatg tagacgtaga aggcgcccag
      841 cagacccagc accgaagcaa ccagcggcgc ccatttcacc caggtcggaa cctcgtgggc
      901 gtcgtgcagc acatggttgg tcggggcgtt gtagatggcg ccgcgccaga actcttcctg
      961 gtggtgaccc acgaagtagt cgacgaagac gaaaccggcc aggacggcgc cgatcgacag
     1021 caccaccaac gggaacagca tgatcagcgg gctctcatgc ggcttgtggt catgcccgtg
     1081 gccatgatcg tcgtgaccgt gatcatccga agcgtgggca gcatggtcgt cgtgcgcatc
     1141 gtggccatga tcgtcatgtc cccagcgcgc gtggccgttg aaggtaaaga aggccaggcg
     1201 ccaggaatag aaggcggtca ggccggccac cagcaggccg atcacgaagg cgaacatggc
     1261 gatcgaattt tcgccgcccg cggcgaaggc cgcc
//
LOCUS       DAOMEO010001077         1292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10503_length_1292_cov_2.362975, whole genome shotgun sequence.
ACCESSION   DAOMEO010001077 DAOMEO010000000
VERSION     DAOMEO010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10503_length_1292_cov_2.362975"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLH31_18530"
     CDS             <1..190
                     /locus_tag="PLH31_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQR91225.1"
                     /translation="KVSPHVLRHAFATHLLEGGADLRVIQTLLGHADIGTTQIYTHVA
                     GDHLAQVVKSKHPLGRKG"
     gene            260..481
                     /locus_tag="PLH31_18535"
     CDS             260..481
                     /locus_tag="PLH31_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HQR91226.1"
                     /translation="MSDETRDSNGTILADGDNVTLIKDLKVKGSGGVTLKRGTLVKNI
                     RLTGDTDEIEANVEKVRGLVLRVEFVKKA"
     gene            complement(486..1142)
                     /locus_tag="PLH31_18540"
     CDS             complement(486..1142)
                     /locus_tag="PLH31_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91227.1"
                     /translation="MILALLLMRHAAAVMPRARREWAAGMHAEMLAIPTPAEALVFAA
                     GCVFAAYQQRISPVRIALAIGRFGVTAVTLLTAGVHIIFLLYWLAIINDLKTHGMDSW
                     AGKFPIFQGLSAAEALHYISLKPSWHVGALIAITAAFAISACSLAHRRFKAVVVAAGT
                     GLSINTANALAMQATDGPYLVHHEIAWLYSLAFVLLVLAALVFRSADKRLTPSAPLAV
                     "
BASE COUNT          256 a          399 c          440 g          197 t
ORIGIN      
        1 aaaggtcagt ccgcatgttt tgcgccacgc cttcgcgacc cacctgctgg agggcggcgc
       61 tgacctgcgg gtcatccaga ccctgctggg acacgccgac atcggcacga cccagatcta
      121 tacccacgtg gccggcgacc acctggccca ggtggtgaag tccaagcatc cgctgggccg
      181 caagggctga cggcagcttg gccttgcctg aaccggcggc gctctttagg aagcccccag
      241 aaaatgtaag gacttcccca tgtcggacga aacccgcgac agcaacggca cgatcctggc
      301 cgatggcgac aacgtcaccc tgatcaagga tctcaaggtc aagggctcgg gcggcgtcac
      361 gctgaagcgc ggcacgctgg tcaagaatat ccgcctgacc ggcgacaccg acgagatcga
      421 ggccaatgtc gaaaaggtgc gcggcctggt gctgcgcgtg gagttcgtca agaaggcctg
      481 accgctcaga ccgccaaggg cgcgctgggc gtcagcctct tgtcggcgct ccggaacacg
      541 agagccgcca ggaccaacag gacgaaagcc agggaataga gccatgcgat ctcgtgatgg
      601 accagataag gtccatcagt ggcctgcata gccagggcgt tggcggtgtt gatagatagg
      661 cccgtgcccg ccgcgacaac cacggccttg aaccggcgat gggccaggga gcaggccgag
      721 atggcgaatg ccgccgtgat ggcgatcaaa gcgccgacat gccaagacgg cttcaggctt
      781 atgtagtgaa gcgcctctgc cgcgctgagg ccctgaaaga tcgggaactt gccggcccaa
      841 ctgtccatac cgtgggtctt caggtcgttg atgatggcca gccagtaaag caggaagatg
      901 atgtggaccc ccgcagtcag gagggtgact gcggtgacgc cgaagcgtcc gatggcgagg
      961 gcgatgcgca cgggcgagat cctttgttga taggccgcga agacacagcc ggccgcgaag
     1021 accaaagctt cggcgggggt ggggatggcc agcatttcgg cgtgcatacc ggccgcccat
     1081 tcgcggcgcg ctcgaggcat tacggcggcg gcgtggcgca ttagcagcaa ggcgaggatc
     1141 atctggccac ctgccgcgct ggcgcgaatt tatctgccgg ttcggcggtc tcgacggcgc
     1201 gctgcagggc cagcgccgca ccctcgtccg tcagccgata ggcgtggcga ggaggacggc
     1261 ccggctgcaa gggctggcgc cattctgcct cc
//
LOCUS       DAOMEO010001078         1291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10514_length_1291_cov_2.402913, whole genome shotgun sequence.
ACCESSION   DAOMEO010001078 DAOMEO010000000
VERSION     DAOMEO010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_1291_cov_2.402913"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1291
                     /locus_tag="PLH31_18545"
     CDS             <1..>1291
                     /locus_tag="PLH31_18545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQR91228.1"
                     /translation="SALERIRRLANAAYEGIVVVQDGRINDANAAFCELVGAPLHELT
                     GKPLLGGILNFNGGEASPDGMRREGVLQPVEGGREIPVEVFSRLMDDGARTETSGLTV
                     LAVRDLRERRSAEEKIRYLAEHDGLTGLLNRNSLQARLTAAIERVEASGETLSVICID
                     LDHFKEANDQHGHLAGDALLVETARRLQDAISAPSFVARLGGDEFIVVQVSGGDQPTA
                     AAELSGRLLDALANPVMFEGQDLAMGASLGVSLFPDDGRTAESLMANADMALYRAKEG
                     GRGIYRFFKREMDDSIRERRALARDLKQGIADGELVVYYQPLARASDGEVCGFEALVR
                     WRHPTRGMIPPLDFIPVAEENGLIAPLGEWVLRRACADAAAWEKPLRIAVNLSPLQLN
                     NPALPALVHEVLISTGLSPHRLELEITESALFKDYQRA"
BASE COUNT          201 a          449 c          429 g          212 t
ORIGIN      
        1 ctcggccctt gaacgcattc gccgcctggc caacgccgcc tatgagggca tcgtcgttgt
       61 tcaggacggc cggatcaacg acgccaacgc cgccttctgc gaactggtcg gcgcgccgct
      121 ccacgaactc acgggcaagc ccctgcttgg cggcattctg aacttcaacg ggggtgaggc
      181 ctcgcccgac ggcatgcgcc gcgaaggcgt gctgcaacca gtcgagggcg gccgcgagat
      241 cccggtcgag gtcttttcgc gcctgatgga cgacggcgcc cgcacggaga catctggcct
      301 caccgtactg gcggttcgcg acctgcgcga gcgtcgctca gccgaggaaa agatccgcta
      361 tctggccgag catgatggtc tgaccggtct gctcaatcgc aactcgctgc aggcgcgcct
      421 gacggccgcc atagagcgcg tcgaggcctc gggcgagacc ctgtcggtga tctgtattga
      481 tctggaccac ttcaaggaag ccaacgacca gcatggccac cttgcgggcg acgccctgct
      541 ggtcgagacc gcgcgccgtc tgcaggatgc catttcggcg ccgtccttcg tcgcgcgcct
      601 gggcggcgac gagttcatcg ttgtgcaggt gagcggcggc gaccagccga cagccgccgc
      661 cgagctgtcg ggccgactgc tcgacgccct ggccaatccc gtgatgttcg aaggccagga
      721 cctggccatg ggcgccagcc tgggcgtcag cctcttcccc gacgacggcc gcaccgccga
      781 gtcgctgatg gccaatgccg acatggcgct gtatcgcgcc aaggagggcg gccgcgggat
      841 ctatcgcttc ttcaagcgcg agatggacga ctcgatccgc gagcgtcgcg ccctggcccg
      901 cgacctcaag cagggcatcg ccgatggcga gctggtggtc tactaccagc ccctcgcccg
      961 cgcctctgac ggcgaagtct gcggattcga agccctggtg cgctggcggc atcccacgcg
     1021 cggcatgatc ccgccgctcg atttcattcc ggtggccgag gaaaacggtc tgatcgcgcc
     1081 gctgggcgag tgggtcctgc gccgcgcctg cgccgacgcc gccgcctggg agaagccgtt
     1141 gcggatcgcg gtgaacctgt cgccgctgca gctgaacaac cccgccctgc ctgctctggt
     1201 gcatgaggtg ctgatctcga cgggcctgtc gccgcaccgg cttgagctgg aaatcaccga
     1261 aagcgcgctg ttcaaggact atcagcgggc c
//
LOCUS       DAOMEO010001079         1288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10554_length_1288_cov_1.738037, whole genome shotgun sequence.
ACCESSION   DAOMEO010001079 DAOMEO010000000
VERSION     DAOMEO010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_1288_cov_1.738037"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLH31_18550"
     CDS             <1..279
                     /locus_tag="PLH31_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR91229.1"
                     /translation="VFADGGYGGDKLKGRLTKVGQWTLQIIKRSDRATGFEVLPRRWV
                     VDRTFAWLGRCRRLAKDYEKTIASAEARIFVANLRILTRRVARYCYPS"
     gene            849..1288
                     /locus_tag="PLH31_18555"
                     /pseudo
     CDS             849..1288
                     /locus_tag="PLH31_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512244.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          218 a          389 c          422 g          259 t
ORIGIN      
        1 gtcttcgccg atggcggcta tggcggcgac aaactgaagg ggcgcttgac caaggtcggc
       61 caatggaccc ttcagatcat caagcgctcc gatcgcgcca ccggcttcga ggttttgccg
      121 cggcgctggg tcgttgatcg caccttcgca tggctcggac gctgccgaag gctcgccaag
      181 gactacgaaa aaaccatcgc cagtgctgag gcgcggatct tcgtcgcaaa tctccgtatc
      241 ctcacaaggc gcgtcgcaag gtactgctat ccttcataga gttctgagtc gggaactgag
      301 ggttcgtgag ccttctctgt atctgttctt cgcgctgtac cgcatggagc cggaaagtag
      361 gctcgcatat agtccggtgg gcgatggcga cttacgtcac tccgcttgag gcgcaattca
      421 cacgggcgat tgaccgcccg tggatggcct acgtcgccat cgtcctcgac gacaccagcc
      481 tgcccgaaaa agggcagcca ttcggtgggg caacggtatt ttcaaacggg agaggttggt
      541 gcgcttttgc ggagaatggt gttgaggata acgaggaact gatcccaagc tttgatggtg
      601 cagtcttttc gcaggaatgg aaggatgcgc tatgggccag gttctccatg ggtgcgccac
      661 gacgacagag gcggtccgtc gagcgatacc gcatagtcat gtctggacga cgcccgctga
      721 tcaagggttt gattcctgca atgggcgatc gcgggtgcgg taatgtctcc ggcctgttga
      781 tgcagtctta tgaccgctgg ccccgatggt gtccgctgat ctgttggctt gatccagaga
      841 gcgagctcga ggttcccgtc gtcctgggac tgcgcctgtt cctgccggag agctggacca
      901 acgatgttga acgcatggtc gccgcggcgt gccggagctt ttctaggcgc cgcgctccaa
      961 gcccgggggc gcccttcagg agatcgaccg gtcatggcgc aaggcgtgcg cttcggcctg
     1021 gtgctggccg atgccggcta agacctgtcc gcgtccttcc gacagggcct gagcgcccgc
     1081 ggcctcgcct gggccgtggg catcccgaat caccagaagg tctatggcgc cgatgtcgcg
     1141 ctcgtcttcc ccgtcgccgg gcgcggccgg ccccgcaaga accagatccc cgaccagctt
     1201 tccgtgcccg ccgaggcggt gctggcccga gcccgatggc gcaagggctc ctggcggcgg
     1261 gcaccaaggg acccttgagt gcgcgctt
//
LOCUS       DAOMEO010001080         1287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10561_length_1287_cov_2.267857, whole genome shotgun sequence.
ACCESSION   DAOMEO010001080 DAOMEO010000000
VERSION     DAOMEO010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10561_length_1287_cov_2.267857"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PLH31_18560"
     CDS             <1..954
                     /locus_tag="PLH31_18560"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HQR91230.1"
                     /translation="NIGLFSLFTALYAARWIFYFDGARQIFGHSVVSMFFGCIPMGLA
                     TIINGFLAFGVPHWGESAVQIAQVLWWADAAMALACGIAIPFMMFTRQTHTIDQMTAV
                     WLLPVVAAEVAAVSGGLLAPHLADTHHQLTVLITSYALWACSVPLAMSILVILVLRMA
                     VHKLPHASMAASSWLALGPIGTGALGLLVMGGAAPAIFAANGLGDYGAAARGIGLIGG
                     VLLWGYGFWWAAMAALITARYLREGLPFNLGWWGYTFPIGVYAVATLKLSTLLPLAAF
                     AIIGGGLVILLAIMWLVVGARTVRGAWRGDLFVAPCLKSGQ"
     gene            990..>1287
                     /locus_tag="PLH31_18565"
     CDS             990..>1287
                     /locus_tag="PLH31_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91231.1"
                     /translation="MAQTLSDSVTHRRLFLAGAGLLAATAFAPRSWAQAGADLRLPGG
                     PSTRPLTDAFPGKRGMILQRTRPPLLETPMSVFDQGVFTPNDQFFVRWHWANVPT"
BASE COUNT          163 a          466 c          409 g          249 t
ORIGIN      
        1 aatatcgggc tcttcagcct gttcaccgcg ctctacgcgg cgcgctggat cttctacttt
       61 gacggcgccc gccagatctt cggccactcg gtcgtgtcga tgttcttcgg ctgcatcccg
      121 atggggctgg ctacgatcat caacggcttc ctggcgtttg gcgtcccgca ctggggagag
      181 agcgcggttc agatcgctca ggtcctgtgg tgggccgatg cggccatggc gctggcctgc
      241 ggcatcgcca ttcccttcat gatgttcacg cggcagaccc acaccatcga tcagatgacg
      301 gcggtctggc tgctgcccgt tgtcgccgcc gaggtggccg ccgtcagtgg cggcctgctg
      361 gcgccccatc tggccgacac acatcaccaa ctgaccgtgc tgatcaccag ctacgcgctc
      421 tgggcctgct cagtgccgtt ggcgatgagc atcctggtca tcctggtgct gcgcatggcg
      481 gtgcacaagc tgccgcacgc cagcatggcc gcctccagtt ggctggcgct tggccccatc
      541 ggcaccggcg cgcttggcct gctggtcatg ggcggcgcgg cgccggcgat cttcgccgcc
      601 aacggactgg gcgactatgg cgccgcggcc cgcggcatcg gcctgatcgg cggcgtgctg
      661 ctctggggct acggcttctg gtgggcggcg atggcggccc tgatcaccgc acgctacctg
      721 cgtgaaggcc tgccgttcaa tctcggctgg tggggctaca ccttccccat cggcgtctat
      781 gcggtggcca ctctcaagct ctccaccctc ctgccgcttg ccgccttcgc catcatcggc
      841 ggtggcctgg tgatcctgct ggcgatcatg tggctggtgg tcggcgcccg caccgtgcgc
      901 ggggcctggc gcggcgacct cttcgtcgcc ccttgcctca agagcggcca atgacctagc
      961 ctgtcacccc tctcaacgcg gaggccctaa tggcccagac cctcagcgac tcagtaaccc
     1021 atcgccgcct gttcctggcc ggcgctggcc tactggcggc cacggccttc gcgccccgga
     1081 gttgggccca ggcgggcgct gatctgcgct taccgggcgg tccatcgacc cggccgctca
     1141 ccgacgcctt ccccggcaag cgcggcatga ttctgcagcg tacccggcct ccgctgctag
     1201 agacgccaat gagcgtcttc gaccagggcg tgttcacccc caacgaccag ttcttcgtac
     1261 gctggcactg ggcaaacgtg ccgaccg
//
LOCUS       DAOMEO010001081         1286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10575_length_1286_cov_2.471974, whole genome shotgun sequence.
ACCESSION   DAOMEO010001081 DAOMEO010000000
VERSION     DAOMEO010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10575_length_1286_cov_2.471974"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1286)
                     /locus_tag="PLH31_18570"
     CDS             complement(66..>1286)
                     /locus_tag="PLH31_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HQR91232.1"
                     /translation="LFGFFWTYLEAGVDGLSGLVIASGDVGLFVYGLLNRLLIVTGLH
                     HILNNVVWFILGDFNGATGDLNRFAAGDPTAGAFMSGFFPVMMFGLPAACLAMLHTAR
                     PERRKAVGGMLGSLALTSFLTGVTEPIEFSFMFLAPVLYAVHALLTGLSMVIMNLLDV
                     KLGFGFSAGLFDYVLNFNKATRPLMLIPVGLVYGAIYYGVFRWVIIRFDLKTPGREPD
                     DAIAAPVARSAGGRGEDFLIALGGAANLASVDACTTRLRLIITGEGSVDEPRLKALGV
                     RGVVRPSERALQVVLGPIADQVASEIRAAMGGAGARTASPTPVAATPAVTGDKAQAER
                     LVAALGGSRNIETLGSCTSRLRVVVLDPLAVDETALKSLGARGVARIGERTIHVVLGP
                     QADALAEAIRLLPA"
BASE COUNT          234 a          432 c          451 g          169 t
ORIGIN      
        1 ggcggcggct ttggcggaag tgatcgcgga cctgggatat ccgtctcctc ttgcggggaa
       61 cgatatcagg ccggcaacag cctgattgct tcggccaggg cgtctgcctg cgggcctagc
      121 accacgtgga tggttcgctc gccgatccgg gccacgccac gcgcgccgag gctcttcagc
      181 gcggtttcat cgaccgccag cgggtccagc accacgacgc gcaggcggct ggtgcaactg
      241 cccagtgtct cgatattgcg cgaaccgccg agggccgcca cgaggcgctc ggcctgggcc
      301 ttgtcgcccg tcacagctgg cgtggcggcg accggcgtcg gcgaggcggt cctggcgccg
      361 gcgccgccca tggccgcgcg gatctcgctg gcgacctgat cggcgatggg gcccagcacg
      421 acctgcagcg cccgctccga gggccgcacg acaccccgta cgccgagggc cttcaggcga
      481 ggctcgtcaa cggagccctc gccggtgatg atcaggcgca ggcgcgtggt gcaggcgtcg
      541 acggaggcga gattggccgc gccgcccagg gcgatcagga aatcctcgcc gcgcccgccg
      601 gccgaacgcg cgaccggcgc ggcgatggcg tcgtcaggct cgcgaccggg ggtcttcagg
      661 tcgaagcgaa tgatcaccca gcggaagacg ccgtaataga tcgcgccata gaccagaccc
      721 accgggatca gcatcagcgg ccgggtggcc ttgttgaagt tcagcacata gtcgaacagc
      781 cccgccgaga agccaaagcc cagcttcacg tccagcaggt tcatgatcac catggacagg
      841 cccgtcagca gggcgtgaac cgcgtacagc accggggcca ggaacatgaa gctgaactcg
      901 atgggctcgg tcacgccggt caggaacgag gtcagggcca gcgagcccag catgcccccc
      961 accgccttgc ggcgctcggg acgggcggtg tgcagcatgg cgaggcacgc ggcgggcagg
     1021 ccaaacatca tcaccgggaa gaagccgctc atgaacgccc cggcggtcgg gtcgccagcc
     1081 gcgaaacggt tcaggtctcc ggtcgcgccg ttgaagtcgc ccaggatgaa ccagaccacg
     1141 ttgttgagga tgtggtgcag gccggtaacg atcagcaggc ggttgagcag gccatagacg
     1201 aacaggccca cgtcgcccga ggcgatgacc aggccgctga gaccgtcgac gccggcctcc
     1261 agataggtcc agaaaaagcc gaacag
//
LOCUS       DAOMEO010001082         1284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10599_length_1284_cov_1.950366, whole genome shotgun sequence.
ACCESSION   DAOMEO010001082 DAOMEO010000000
VERSION     DAOMEO010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10599_length_1284_cov_1.950366"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLH31_18575"
     CDS             complement(<1..217)
                     /locus_tag="PLH31_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HQR91233.1"
                     /translation="MSSIRLRLFVTLALVTALVWGGAVVWVEIQTRAEVQRVLDRRLM
                     ESARMVSSLMQAGSVRPGRTLSAINAAG"
     gene            complement(214..867)
                     /locus_tag="PLH31_18580"
     CDS             complement(214..867)
                     /locus_tag="PLH31_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR91234.1"
                     /translation="MRLLAIEDDPVLADGLQVGLSACGWTVEVVGRVEDALAALESSA
                     FDAVVLDLGLPDGDGLSVLRFVRERDMDVGVVVLSARDDSRDRIVGLDAGADDYVGKP
                     FDLDELAARLRAVRRRLLGRSTPALRHGGVTIDPKSRLAWREEGDLPLSRREFAILDA
                     LMERPAQVLSRSQLEERLYGWQEEIGSNAVEVHIHYLRAKLGAGFIRTVRGVGYTLQ"
     gene            930..>1284
                     /locus_tag="PLH31_18585"
     CDS             930..>1284
                     /locus_tag="PLH31_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HQR91235.1"
                     /translation="MRFFHRLLPAALALLSFALPGSALGQAPLSPDKAFALTVTREAD
                     GDLAFAWDIAPGHYLYRDHTVASTPGGKESLPLELAAGETKDDPGFGVVDVWHDAGRA
                     TLSAQTLTQAGEPTSV"
BASE COUNT          222 a          452 c          406 g          204 t
ORIGIN      
        1 tccctgctgc gttaatcgcc gaaagcgtgc gccccgggcg cacgctgccg gcctgcatca
       61 gggacgaaac catgcgggcc gactccatca ggcgccggtc cagcacccgc tgcacttcgg
      121 cccgggtctg gatttcgacc cagacgaccg cgccgcccca gaccagggcg gtcacgagcg
      181 cgagagtgac gaacagacgc aggcggatag agctcattgc agggtgtatc ccacgccgcg
      241 gacggtgcgg atgaagccgg ctcccaactt ggcccgcaga tagtggatgt gcacttccac
      301 ggcgttggag ccgatctcct cctgccagcc gtacaggcgt tcttccagct gactgcgcga
      361 gaggacctgt gcgggccgtt ccatcagggc atccaggata gcgaactcac gtcttgaaag
      421 gggcaggtcc ccctcttcac gccaggccag acgcgacttg ggatcgatgg tcaccccgcc
      481 gtggcgcaag gcgggggtcg aacgtccgag caggcgacgc cgcaccgcgc gcaatcgcgc
      541 ggccagctcg tcgagatcga acggtttgcc gacataatcg tccgcaccgg cgtccagacc
      601 cacaatgcgg tcccgactgt catcgcgcgc cgacaagacc accacgccga catccatgtc
      661 gcgctctcga acgaaacgca gcaccgaaag gccgtcgccg tcaggaagac ccaggtccag
      721 cacgaccgca tcgaacgccg agctttccag cgccgcgagg gcgtcctcga cccgcccgac
      781 gacctcgacc gtccagccgc aggcgctgag cccgacctgg aggccgtcgg cgagaacggg
      841 atcgtcttct atggcgagca atctcatgcg gtgtgacgtg caagctccgg cttaagcctg
      901 acttaagctc gcttgccgac gagatggcca tgaggttttt ccaccgactg ctgcccgccg
      961 ctctcgctct tctgtccttc gccctgccgg gttcggcctt gggtcaggct ccgctttcgc
     1021 ctgacaaggc cttcgccctg acggtgacgc gtgaggctga cggcgacttg gccttcgcct
     1081 gggacattgc gcctggccat tatctctatc gtgaccacac cgtcgccagc acgccgggcg
     1141 ggaaggagtc tctgccgctg gaactggccg ccggcgaaac caaggacgat ccgggcttcg
     1201 gcgtcgtcga cgtctggcac gacgcgggtc gcgcgaccct gtcggcgcag acgttgacgc
     1261 aggccggcga gccgacgtcg gtca
//
LOCUS       DAOMEO010001083         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10611_length_1283_cov_2.649837, whole genome shotgun sequence.
ACCESSION   DAOMEO010001083 DAOMEO010000000
VERSION     DAOMEO010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10611_length_1283_cov_2.649837"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLH31_18590"
     CDS             <1..583
                     /locus_tag="PLH31_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQR91236.1"
                     /translation="LGQGVAILKDLAAHPATARHVAFKIARHFVADDPPPALVARLEQ
                     SFLKSGGRLDRVALTLVEAPEAWEPAATKFKTPYEFLISSWRLIGAQPSAFERITVPL
                     IGLGQRGFSPPSPKGWSDMAGEWCSPDGLIKRLQWSQAFAAVVADKLDPNEAASAALG
                     ARLTAPTAQAVARAETRREAIALLLMSPEFQRR"
     gene            580..>1283
                     /locus_tag="PLH31_18595"
     CDS             580..>1283
                     /locus_tag="PLH31_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQR91237.1"
                     /translation="MTDRLMMNRRRVLASAAGLGLSVTFLGRPALADTDGALDKRKLV
                     VVICRGGMDGLSVSPPVGDADYQGLRRGIAFKPEDVLKLDGTFGLHPALTSVHALALK
                     GQARIAPAVATPDRARSHFEAQDVLESGSAQVYATTSGWLNRALEALPASRKAQGLYV
                     GATTPLILRGKVQAASWSPGKGVDETARLPTLLQDLYKTDPLMGPAFARGLETESMAQ
                     VAMTAMATPVEPLVAAG"
BASE COUNT          179 a          486 c          430 g          188 t
ORIGIN      
        1 gctcggccag ggcgtggcca tcctgaagga cctggccgct catccggcca cggcgcggca
       61 cgtcgccttc aagatcgccc gccacttcgt cgccgacgat ccgccgcccg ccctggtggc
      121 gcggctggag cagagcttcc tcaagagcgg cggccggctg gaccgtgtgg ccctcaccct
      181 ggtcgaggcc ccggaagcct gggagccggc ggcgaccaag ttcaagacgc cctacgagtt
      241 cctgatctcg tcctggcggc tgatcggcgc ccagccctcg gccttcgagc ggatcaccgt
      301 gcccctgatc ggcctgggcc agcgcggctt ttcacccccc tcgcccaagg gctggtcgga
      361 catggccggc gaatggtgct cacccgacgg cctgatcaag cggctgcagt ggagccaggc
      421 cttcgccgcc gtggtcgccg acaagctgga tcctaacgag gccgcctcgg ccgccctggg
      481 cgcccgactg accgccccca ccgcccaggc cgtggcgcgg gccgaaaccc gtcgagaggc
      541 catcgccctg ttgctgatga gtccggagtt ccagcgccga tgaccgatcg cctgatgatg
      601 aaccgtcgcc gcgtgctggc ctccgccgca ggccttggcc tgtcggtgac cttcctgggc
      661 cgccccgccc tggccgacac cgacggcgcc ctggacaagc gcaagctggt cgtcgtcatc
      721 tgccggggcg gcatggacgg cctctcggtc agtccgccgg tcggtgacgc ggactatcag
      781 ggcctgaggc gcggcattgc cttcaagccc gaggatgtcc tgaagctgga cggaaccttc
      841 ggcctgcacc ccgccctgac ctcggttcac gccctggcgc tgaagggcca ggcccggatc
      901 gcgccagccg tggcgacgcc cgaccgcgcc cgctcgcatt tcgaagccca ggatgtgctg
      961 gaaagcggct cggcccaggt ctatgcgacc acgtcaggct ggctgaaccg ggcgctggaa
     1021 gccctgcccg cctcccgcaa ggcgcagggt ctgtatgtcg gcgccaccac gcccctgatc
     1081 ctgcgcggta aggtgcaggc ggccagctgg tcccccggca agggcgtgga cgagaccgcc
     1141 cgcctgccca ccctgctgca ggacctctac aagaccgacc ccctgatggg tccggccttc
     1201 gcccggggtc tggaaaccga gagcatggcc caggtcgcca tgaccgccat ggccacgccg
     1261 gtcgaaccgc ttgtggcggc ggg
//
LOCUS       DAOMEO010001084         1281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10642_length_1281_cov_1.692496, whole genome shotgun sequence.
ACCESSION   DAOMEO010001084 DAOMEO010000000
VERSION     DAOMEO010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_1281_cov_1.692496"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLH31_18600"
     CDS             <1..427
                     /locus_tag="PLH31_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HQR91238.1"
                     /translation="RETHAKAKALRKALTPPEAMLWSRLRSRQTDGPRIRRQHAVGPY
                     IADFYCSAARLVIEIDGWGHNLGDQPQHDVRRDAWMEAQRLTVVRYPASEVLADPTGV
                     ADGIWDTCIDLMRERQTPHAPSVTSAIRTAPPPSQAGQE"
     gene            complement(430..>1281)
                     /locus_tag="PLH31_18605"
     CDS             complement(430..>1281)
                     /locus_tag="PLH31_18605"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQR91239.1"
                     /translation="QEQSDPDPARPAQGPPPADHLGRPCRHRRYPDGQWWATFLAVRP
                     YGDDLYNTGRETFLLPVEWKDGWPVILENGKTIPYAATAPNLPAPKVLPPPTTGAFKA
                     VETFKGPDLPLNWMTIRIPTERWWTVGDGALTLQARPDKLTDQPSFWGRRQQHINAAA
                     QVTVDFKPQADGDRAGLTALQSDEFYYFLGVVRSGGKPVLRLERRAGASDPVDGVVVA
                     ETPLAGAGPVDLKITARAGEYHFAYAVKPGDWKVLKAGADGTMLSTRKAGGFVGAMFG
                     VYANGAK"
BASE COUNT          199 a          436 c          440 g          206 t
ORIGIN      
        1 aagagaaact cacgccaagg ccaaagccct ccgcaaggct ctaactccgc cagaggccat
       61 gctctggtcg cgcttgagat cacgccagac ggacggcccg cgcattcgcc gccagcacgc
      121 ggtcggcccc tacatcgccg acttctattg ctcggccgct cgtctggtga tcgagatcga
      181 cggatgggga cacaacctgg gagatcagcc tcagcatgac gtgcggcgag acgcgtggat
      241 ggaggctcag aggctgacag ttgtgcgcta tccggcctca gaggtcctag cagaccccac
      301 cggcgtggcc gatgggatct gggatacgtg cattgatctg atgcgagagc ggcaaactcc
      361 gcacgccccc tccgtcacgt cggctatccg caccgcgcca ccgccctcgc aggcggggca
      421 ggaatagagc tatttcgccc cgttggcata caccccgaac atcgccccga cgaagccgcc
      481 ggccttccgg gtcgacagca tcgtgccgtc ggcgccggcc ttgagcacct tccagtcgcc
      541 cggcttcacc gcataggcga agtgatactc tccggcccgc gcggtgatct tcaggtcgac
      601 cggaccagcg cctgccaggg gcgtctcggc caccaccacg ccgtcgacgg ggtcgctcgc
      661 tccggccctg cgctccagtc gcaggaccgg cttgccgccc gagcgcacca cgcccaggaa
      721 gtagtagaac tcgtcgctct gcagcgccgt caggccggcg cggtcgccgt cggcctgggg
      781 cttgaagtcg accgtcacct gggcggcggc gttgatgtgc tgctggcggc ggccccagaa
      841 cgagggctgg tcggtcagct tgtcgggccg cgcctgcagg gtcagggcgc cgtcgccgac
      901 ggtccaccag cgctcggtcg gaatgcggat ggtcatccag ttcagcggca ggtccggtcc
      961 cttgaaggtc tccacagcct tgaaggcccc ggtcgtcggc ggcggcaaga ccttgggcgc
     1021 gggcaggttc ggggccgtgg cggcataggg gatggtcttg ccgttctcca ggatcaccgg
     1081 ccagccgtcc ttccactcga ccggcagcag gaaggtctcg cggccggtgt tgtagaggtc
     1141 gtcgccatag ggccgcacgg ccaggaaggt ggcccaccac tggccgtcgg ggtatcgacg
     1201 atgtcggcat ggccggccga ggtgatcggc agggggcggt ccctgggcag gccgcgctgg
     1261 gtcaggatcg gattgttctt g
//
LOCUS       DAOMEO010001085         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10645_length_1280_cov_3.229388, whole genome shotgun sequence.
ACCESSION   DAOMEO010001085 DAOMEO010000000
VERSION     DAOMEO010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10645_length_1280_cov_3.229388"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>209)
                     /locus_tag="PLH31_18610"
     CDS             complement(<1..>209)
                     /locus_tag="PLH31_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQR91240.1"
                     /translation="AERIGIPHYVLDYESRFKEQVIEEFADAYLRGETPIPCVRCNQT
                     VKFRDLLDVARDLGAEAMATGHYVQ"
     assembly_gap    210..219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<220..471)
                     /locus_tag="PLH31_18615"
     CDS             complement(<220..471)
                     /locus_tag="PLH31_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQR91241.1"
                     /translation="MAAMDADILDHALSDRAVEMARDAVGLPAGARVVVAMSGGVDST
                     VTAALLARAGYDVVGVTLQLYDHGAAIAKKGACCAGQDIL"
     gene            complement(506..>1280)
                     /locus_tag="PLH31_18620"
     CDS             complement(506..>1280)
                     /locus_tag="PLH31_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQR91242.1"
                     /translation="TAMRSAQAKINGLIDQTKLVSNRLDMQETGVNQMADAVGSAKGA
                     IENAIAAGNAATLMQQLEAAFTNTVQGLNTKSNGRYVFGGAKTDTPPTSATTMADLTI
                     AAQTSDLFHNDQYIATNRIDEQTTVQTGLLADDLGTDIFEAFKQIQSYVEANGPFTGK
                     LTENQTQFLNGMRATFSAAYSDAVNSQGKNGLVQKRFENAGVELQDQADTLTGMVGGI
                     VDVDMAEAVTRLEAAQLAVQASAQVFASLQSSSLLNVLK"
BASE COUNT          186 a          394 c          440 g          250 t
ORIGIN      
        1 ctgcacgtaa tgccccgtcg ccatcgcctc ggcgcccagg tcgcgggcca cgtccagcag
       61 gtcgcggaac ttgacggtct ggttgcagcg cacgcacggg atgggcgtct cgccgcgcag
      121 ataggcgtcg gcgaactcct cgatcacctg ttccttgaac cggctttcgt agtcgagcac
      181 gtagtgcggg atgccgatgc gctcggcggn nnnnnnnnnc aggatgtcct ggccggcgca
      241 gcaggcgccc ttcttggcga tcgccgcgcc gtggtcatag agctgaaggg tcacgcccac
      301 cacgtcatag ccggcgcgag ccagcagggc ggcggtgacg gtggagtcca cgccgccgga
      361 catggccacg accacccgcg cgccggcggg caggcccacg gcgtcgcggg ccatctcgac
      421 cgcgcggtca gaaagggcat gatccagaat gtcggcgtcc atcgcggcca tatagcgact
      481 taagggccgc tttgcgaggt ttggcctatt tcagcacgtt cagcagcgac gagctctgca
      541 ggctggcgaa cacctgggcc gaggcctgga ccgccagctg ggcggcctcc aggcgtgtga
      601 ccgcttcggc catgtcgaca tcgacgatcc cgccgaccat accggtcagg gtgtcggcct
      661 gatcctgaag ctcgacaccg gcgttctcga agcgcttttg cacgagaccg tttttgccct
      721 gcgaattgac cgcgtcgctg taggcggcgc tgaacgtcgc tctcatgcca ttgaggaact
      781 gggtctggtt ttcggtcagc ttgccggtga acgggccgtt ggcctcgaca tagctctgga
      841 tctgcttgaa ggcctcgaaa atgtcggtcc ccaggtcgtc ggccagcagg ccggtctgga
      901 cggtggtctg ctcgtcgatc cggttggtgg cgatgtactg gtcgttgtgg aacaggtccg
      961 aggtctgggc cgcgatggtc aggtcggcca tggtggtggc cgaggtgggc ggcgtatcgg
     1021 tcttggcgcc gccgaacacg tatcgaccat tggacttggt gttcaggccc tggaccgtat
     1081 tggtgaacgc cgcttccagc tgctgcatca gggtggcggc gttgccggcg gcgatggcgt
     1141 tttcaatggc gcctttggcg ctgcccaccg cgtcggccat ctggttgacg ccggtctcct
     1201 gcatgtccag acgattggag accagcttgg tctggtcgat cagaccattg atcttggcct
     1261 gggcgctccg catggcggtg
//
LOCUS       DAOMEO010001086         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10647_length_1280_cov_2.811429, whole genome shotgun sequence.
ACCESSION   DAOMEO010001086 DAOMEO010000000
VERSION     DAOMEO010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10647_length_1280_cov_2.811429"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..679)
                     /locus_tag="PLH31_18625"
     CDS             complement(131..679)
                     /locus_tag="PLH31_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQR91243.1"
                     /translation="MSDAITAIPLTRIDGQTDTLANHSGKVLLIVNVASKCGLTPQYE
                     GLEALYRDKQANGLEVLAFPANNFNGQEPGADAEIATFCKTSYDVTFPLYAKISVKGE
                     DIHPLYAELTAAKPEAVGDGPMRERLAGYGIATGGPGEIVWNFEKFLVGRDGRVLDRF
                     APDVDAADPRLIDSVEKALSAD"
     gene            800..1198
                     /locus_tag="PLH31_18630"
     CDS             800..1198
                     /locus_tag="PLH31_18630"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HQR91244.1"
                     /translation="MTRAPFDIIREFLDAWSTGPEPLEKAIRDLFRADTEWINMGLSK
                     TVGPDQALAMGAKFEAAMGYAKITVETLHIAANGNVVLTERMDRLIAADGREIGAFAL
                     AGIFELDHDGKLIRWRDYTDPSAVAAIRGH"
BASE COUNT          229 a          425 c          393 g          233 t
ORIGIN      
        1 atttcgccga cgcctccgac gcctgggccg cccaggtctc cggcccccac gccaagatca
       61 cggcgaaggt gcgctgatga tcgccgggga aggcgcgcaa gccctccccg gcggtcgcca
      121 tttgtcggcg tcagtcggcc gacagcgcct tctcgaccga gtcgatcaag cgcggatcgg
      181 cggcgtcgac gtcgggcgcg aagcggtcga gcacccggcc gtcacgccca accaggaact
      241 tctcgaaatt ccagacgatc tcgccgggcc cgcccgtcgc gatgccatag cccgccagac
      301 gttcgcgcat cggtccgtcg ccgaccgcct cgggcttcgc cgccgtcagt tcggcataga
      361 gcgggtggat gtcctccccc ttgacgctga tcttggcgta gagcgggaac gtcacgtcgt
      421 agctggtctt gcagaaggtc gcgatctcgg cgtcggcgcc cggctcctgg ccgttgaagt
      481 tgttggcggg gaaagccaac acctccagac cgttcgcctg cttgtcgcga tagagcgcct
      541 cgagcccttc atactggggc gtcaatccgc acttcgacgc cacattgacg atcagcagaa
      601 ccttgccgga atggttggcc agggtgtcgg tttgaccgtc gatacgggtc aggggaatgg
      661 cggtaatcgc gtcgctcatg tcggctccat catgtctgga tggcggggcc atctctgtgg
      721 cgcagcttac caaatcgccc cggcgaccgc gctcggaaac ccaggttttg ccccctgaaa
      781 atcgaaggaa catcatcttg tgacccgcgc cccgttcgac atcatccgcg agtttctcga
      841 cgcttggtcc accgggcccg aaccgctcga aaaggcgatc cgagacctat ttcgggccga
      901 caccgaatgg atcaacatgg gcctgtcgaa gacggtcggt cccgatcagg ctctggccat
      961 gggcgccaag ttcgaggccg ccatgggata cgccaagatc acggtcgaga ccctgcatat
     1021 cgccgccaac ggcaatgtcg tcctgaccga gaggatggac cggctgatcg ccgccgacgg
     1081 gcgagagatc ggcgccttcg ccctggccgg cattttcgaa ctggatcacg acggcaagct
     1141 gatccgctgg cgagactata ccgacccatc cgccgtcgcc gcgattcgcg gacactgagg
     1201 ggctggatcg accttacgtt tgatggacat ttccatcaga atgctattct gtatctgaaa
     1261 aatatcgcgc ccgcgaggcc
//
LOCUS       DAOMEO010001087         1280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10651_length_1280_cov_1.807347, whole genome shotgun sequence.
ACCESSION   DAOMEO010001087 DAOMEO010000000
VERSION     DAOMEO010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_1280_cov_1.807347"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLH31_18635"
     CDS             complement(<1..162)
                     /locus_tag="PLH31_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQR91245.1"
                     /translation="MSERRAYLYKGVGETVGVVTLDGRPERLIATWDGDDPLDVEGVR
                     GVARIRKIEK"
     gene            complement(159..755)
                     /locus_tag="PLH31_18640"
     CDS             complement(159..755)
                     /locus_tag="PLH31_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HQR91246.1"
                     /translation="MASATAVQPALVLASASPRRLDLLAQVGVTPDRVDPADIDETPL
                     RDETPRLHALRLARDKARAVASRSPGDFVLAADTVVAVGRRILPKAETEEQAIRCLAL
                     LSGRNHKVLTAVAVIAPDGREASRLVETRVQVKQLSDIEKADYLAGGEWRGKAGGYAV
                     QGVAGGFIIDLHGSYTGVVGLPLYETLNLLRGLGWSGK"
     gene            complement(761..979)
                     /gene="infA"
                     /locus_tag="PLH31_18645"
     CDS             complement(761..979)
                     /gene="infA"
                     /locus_tag="PLH31_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQR91247.1"
                     /translation="MAKEELLEFPGTVSELLPNATFRVTLENAHEIIAHTAGKMRKNR
                     IRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            complement(1077..>1280)
                     /locus_tag="PLH31_18650"
     CDS             complement(1077..>1280)
                     /locus_tag="PLH31_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HQR91248.1"
                     /translation="VSLTPEAQHRAVELARDRAVDIEYWPTHDPTLVDGSREARLEAY
                     REVRDGLAAAIKRRFGAPSTFGG"
BASE COUNT          209 a          441 c          424 g          206 t
ORIGIN      
        1 cttttcgatc ttgcggatgc gggccacgcc gcgaacacct tcgacgtcca gcggatcatc
       61 accgtcccag gtggcgatca gccgctcggg gcgaccatcc agggtgacga cgccgacggt
      121 ctcgccgacg cctttataga ggtatgcgcg tctctcactc atttgccact ccagcccagg
      181 ccgcgcagca ggttcagggt ctcgtacagc ggcaggccga ccacgccggt ataggagcca
      241 tgcaggtcga tgatgaatcc gcccgccacg ccctggacgg catagccgcc ggccttgccg
      301 cgccattcgc cgccagccag atagtcggcc ttctcgatat cggagagctg cttgacctgc
      361 acccgcgtct cgaccagcct cgaggcctcg cggccatcag gggcgatcac ggccacggcg
      421 gtcagcacct tgtgattgcg gccagacaga agcgccagac accggatcgc ctgctcctcg
      481 gtctcggcct tgggcagaat gcgccgaccc acggcgacca cggtgtcggc ggccaggacg
      541 aagtcacccg gcgaccggct agcgaccgcc cgcgccttgt cccgcgccag gcgcagggca
      601 tggagccgcg gggtttcgtc gcgcaggggc gtttcatcga tgtcggcggg atcaacccgg
      661 tcgggcgtga cgccgacctg cgccagcagg tcaaggcgcc ggggactcgc gctggccaga
      721 accagcgccg gctggacggc cgtagcggaa gccatcgccc ctacttgaag cggtaggtga
      781 tacggccctt ggtcaggtcg tagggggtca tctcgaccag caccttgtcg ccggccagca
      841 cgcggatgcg gttcttgcgc atcttgccgg cggtgtgggc gatgatctcg tgggcgtttt
      901 ccagcgtcac gcgaaaggtc gcgttgggca gcagttcgct gaccgtaccg ggaaactcga
      961 gaagttcttc cttagccatc aggcctctca attggagcaa aacagatagc cgcgaggacg
     1021 ccccgaccgg atggtcgaga cgtgagccgt cgcggcgagg cgcctctata gcgcattcac
     1081 cccccgaacg tcgatggcgc gccgaaacgt ctcttgatgg cggccgcgag gccgtcgcgg
     1141 acctcgcgat aggcctccag ccgcgcctct cgcgagccgt ccaccagggt gggatcgtgg
     1201 gtcggccagt actcgatatc gaccgcccgg tcccgcgcca gctcgacggc gcgatgctga
     1261 gcctcgggcg tcagcgagac
//
LOCUS       DAOMEO010001088         1278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10672_length_1278_cov_2.311529, whole genome shotgun sequence.
ACCESSION   DAOMEO010001088 DAOMEO010000000
VERSION     DAOMEO010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10672_length_1278_cov_2.311529"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..233)
                     /locus_tag="PLH31_18655"
     CDS             complement(87..233)
                     /locus_tag="PLH31_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91249.1"
                     /translation="MKRIFSTAALLLLASASAAYAAAPDAVTKAVQACCAFGAACCNG
                     GPCC"
     gene            complement(282..554)
                     /locus_tag="PLH31_18660"
     CDS             complement(282..554)
                     /locus_tag="PLH31_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91250.1"
                     /translation="MLLSALLLFAAPAMPSAAAMSQREFCEEMPCHDRGKKAPCPEAC
                     LIACQVIIAPEALIVRSAEIGSAPIMPMISSLLPGRVLAPELPPPR"
     gene            complement(669..1064)
                     /locus_tag="PLH31_18665"
     CDS             complement(669..1064)
                     /locus_tag="PLH31_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91251.1"
                     /translation="MPQGANSAQPHHVDLHVGALIRTRRKALGISQSELADSLGLTFQ
                     QVQKYERGSNRVSSSKLYEIALKLETPLASFFEGLETPVGEGPFAGGLTDFLNQRGAH
                     ELVAAFTVMSPALRRQLVSLAMAMAETDD"
BASE COUNT          236 a          376 c          424 g          242 t
ORIGIN      
        1 ctgtcgagac ttcggtgctc tgagcctgaa ggcaaaacgc ccgcctattt cgaggcgggc
       61 gtcctgtcta gccggcgtgg gctaattcag cagcaaggac cgccgttgca gcatgccgcg
      121 ccaaaggcgc agcacgcttg gacggctttt gtcacggcgt ccggcgcggc ggcataggcg
      181 gccgaggcgg aagccagcag cagcaaggct gcggtcgaga agatgcgttt catgatgatt
      241 ttccgatttc agatggagag cgttttcgtc tgaactcggt gtcaccgggg gggcggaagc
      301 tccggcgcca ggacgcgccc cggaagtagt gaagagatca tcggcatgat gggcgcagaa
      361 ccgatctccg ctgaacggac gatcagggct tcgggcgcga tgatgacctg gcaggcaatc
      421 aggcacgcct cggggcaagg agccttcttg ccccggtcat ggcagggcat ttcctcgcag
      481 aactcgcgtt gcgacatcgc ggcggcggaa ggcatggctg gcgcggcgaa caacagcagg
      541 gcactcagga gcatcagccc ccgcgccaac cagtttggtc ctgtcgccct tagcgtcatg
      601 cccaccttct tagaccattg gcgcatgcgg ggaagccgtc ttggccctta agggcgcggc
      661 ttggacgcct agtcgtcggt ctcggccatg gccatggcca agctcaccag ctgccgacgt
      721 aatgctggcg acatcactgt gaaagccgcc accagttcgt gggcgccgcg ctgatttagg
      781 aaatcggtca ggccgccggc aaagggtccc tcgccgactg gcgtttccaa gccttcgaag
      841 aatgacgcca gcggcgtctc aagcttcaag gcgatctcat agagcttgga tgaacttacg
      901 cggttagacc cccgctcgta cttttggacc tgctgaaagg tcaggcccag cgagtccgca
      961 agctcagact gtgaaattcc caaagccttg cgccgcgtcc tgatcaaggc ccccacatgt
     1021 agatcgacat ggtggggctg cgcgctattg gcgccttggg gcacaagctt cttcattttg
     1081 ttggtcttgg ctcgatgggg atggcggggc tgttggtagg taagtcgccg gggcctattc
     1141 gtcgagcgca gggattggcg ggcgccaccg tgagaccaga tcaaggtgct cctcgcgcct
     1201 ggtcgagcga gcaacattga ggcggatgta gagcccctgg gcgctgtgag atcgcaccgc
     1261 agcgtcccat tgctggcg
//
LOCUS       DAOMEO010001089         1278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10673_length_1278_cov_2.259199, whole genome shotgun sequence.
ACCESSION   DAOMEO010001089 DAOMEO010000000
VERSION     DAOMEO010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10673_length_1278_cov_2.259199"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLH31_18670"
     CDS             complement(<1..337)
                     /locus_tag="PLH31_18670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517397.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HQR91252.1"
                     /translation="MVGILEDKDRIFTNLYGLHDWGLEGAKTRGCWNGTKDILDAGRD
                     WIIDNMKNSGLRGRGGAGFGTGMKWSFMPKEVKDGRPHYLVVNADESEPGTCKDREIM
                     RHDPHLLIEG"
     gene            complement(341..607)
                     /locus_tag="PLH31_18675"
     CDS             complement(341..607)
                     /locus_tag="PLH31_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91253.1"
                     /translation="MIDDSDFNTRKADYLETIRGLGRRERGIGFVACLLGALLLIWGR
                     VVAGAPDWAVVLGLVTIAAGWLLFAYVILRRTRYVRAHPFDPQS"
     gene            complement(604..831)
                     /locus_tag="PLH31_18680"
     CDS             complement(604..831)
                     /locus_tag="PLH31_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91254.1"
                     /translation="MNADAVLQKKRLTAMLAIDAVCFVLAGAAIVGHVAMGIGWLLPA
                     FLVLVAAGLGAQIWFVLGWMRAVKREEGASS"
     gene            complement(831..>1278)
                     /locus_tag="PLH31_18685"
     CDS             complement(831..>1278)
                     /locus_tag="PLH31_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQR91255.1"
                     /translation="VMFQLQPVGKVAFVQLCGTTPCQLRGALDLRQVLKDKIGEAHHV
                     SADGKFSWEEVECLGACCNAPMAAINDYYYEDLTPESLAQILDDFAAGKSPQPGSYEG
                     RKASEPKDSIKTLTDPKLYDGSLAKKIKIPNLPEKPKKAPATEPTA"
BASE COUNT          231 a          423 c          399 g          225 t
ORIGIN      
        1 agccttcgat caggagatgc ggatcatgcc gcatgatctc ccggtccttg caggtgcccg
       61 gctcggattc gtcggcgttg accaccagat agtgcggacg accgtccttc acttccttgg
      121 gcatgaacga ccacttcatg ccggtgccga agcccgcgcc gccccggccg cgcaggccgg
      181 agttcttcat gttgtcgatg atccagtcgc gacccgcgtc caggatgtcc ttggtgccat
      241 tccagcagcc acgcgtcttc gcgccctcga ggccccaatc gtggagaccg tagaggttcg
      301 tgaaaatgcg gtccttgtct tcgaggatac cgaccatgac tcagctttgc ggatcgaatg
      361 gatgggcgcg cacataccgc gtccgacgaa ggatgacata ggcgaaaagc agccaaccgg
      421 cggcaatcgt cacaagaccc agaaccacgg cccaatccgg cgcgccggcg acgacccggc
      481 cccagatcag cagtagcgcg cccaggaggc aggccacgaa gccgatgccg cgttcacggc
      541 gaccaaggcc ccgaatggtc tcaagatagt ccgccttgcg cgtgttgaag tcgctgtcgt
      601 cgatcatgac gaagccccct cttcccgctt cacggcgcgc atccagccca ggacgaacca
      661 gatctgagcg ccaaggccgg cggcgaccag aaccaggaaa gccggcaaca gccagccaat
      721 ccccatggcc acgtgaccga cgatcgccgc gccggccagc acgaagcaga ccgcgtcgat
      781 ggcgagcatc gcggtcagtc gcttcttctg gaggacggcg tcggcattca tcaggcggtt
      841 ggctccgtcg ccggggcctt cttgggcttt tcggggaggt tcgggatctt gatcttcttg
      901 gccagcgagc cgtcatagag cttgggatcg gtcagggtct tgatcgaatc cttgggctcg
      961 gaggccttgc gaccctcata gctacccggc tgcggcgact tgcccgcggc gaagtcgtcg
     1021 agaatctggg ccaggctctc cggcgtcagg tcttcgtaat agtagtcgtt gatggcggcc
     1081 atcggggcgt tgcagcacgc gcccaggcac tcgacctctt cccagctgaa cttgccgtcg
     1141 gccgagacat gatgggcttc gccgatcttg tccttcagca cctggcgcag gtccagcgcg
     1201 ccgcgcagct ggcagggcgt ggtgccgcac agctgcacga aggcgacctt gccgaccggc
     1261 tgcagctgga acatcacg
//
LOCUS       DAOMEO010001090         1276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10696_length_1276_cov_2.434070, whole genome shotgun sequence.
ACCESSION   DAOMEO010001090 DAOMEO010000000
VERSION     DAOMEO010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_1276_cov_2.434070"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLH31_18690"
     CDS             complement(<1..535)
                     /locus_tag="PLH31_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR91256.1"
                     /translation="MTRVAFVTGGTRGIGRAICERLVADGMKVAAGYSGNEEAAAACA
                     KDLGVMVVKGNVGSFEDCAAAVKKVEAELGPIDVLINNAGITRDGMLHKMTYEQWSEV
                     IRVNMDSAFNMTRPVIEGMRERNWGRIINISSINGQKGQMGQTNYSAAKAGLIGFTKA
                     LALENAKKGVTVNVICPG"
     assembly_gap    640..649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<650..>1276)
                     /locus_tag="PLH31_18695"
     CDS             complement(<650..>1276)
                     /locus_tag="PLH31_18695"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQR91257.1"
                     /translation="WQITRGDQDSFAVASQNKAEAAQKAGKFDAEIVPVTIKGRKGDT
                     IVDKDEFIRHGVTIDAISGLRPAFNKEGSVTAANASGLNDGAAALVLMSADEAKKRGL
                     TPLARIASWANAGVEPEIMGTGPIPASKKALEKAGWTVADLDLIESNEAFAAQSLCVV
                     QELGLDTAKVNVNGGAIAIGHPIGASGARILTTLLHEMKRSGAKKGLAT"
BASE COUNT          185 a          408 c          407 g          266 t
ORIGIN      
        1 agccggggca gatcacattg accgtcacgc ccttcttggc gttttccagg gccagggcct
       61 tggtgaagcc gatcaggcca gccttggcgg ccgaatagtt ggtctggccc atctggccct
      121 tctggccgtt gatcgagctg atattgatga tccggcccca gttgcgctcg cgcatgccct
      181 cgatgaccgg acgggtcatg ttgaaggctg agtccatgtt gacccggatc acttcggacc
      241 actgctcgta ggtcatcttg tgcagcatgc cgtcacgggt gatgccggcg ttgttgatca
      301 gcacgtcgat cggacccagt tcggcctcga ccttcttgac ggcggccgcg cagtcttcga
      361 acgagccgac attgcccttc acgaccatca cgcccagatc cttggcgcag gcggcggcgg
      421 cttcttcatt gcctgaatag ccagccgcga ccttcatgcc gtcggccacc aggcgctcgc
      481 agatcgcgcg gccaatgccc cgggtgccgc cggtcacgaa tgcaactctc gtcatactgt
      541 ctctcccagt ttctttttat ggtcccggtg atcaacgcgc cggaaaaggc ccggcgtgtt
      601 gatcaggggc aatttttggc tcagacggcc tcgacgcacn nnnnnnnnng gtcgccagac
      661 ccttcttggc gcccgagcgc ttcatctcgt gcagcagggt ggtgaggatg cgtgcgcccg
      721 aagcgccgat cgggtgaccg atggcaatgg cgccgccatt gacgttgacc ttggcggtgt
      781 cgagacccag ctcctgaacc acgcacagcg actgggcggc gaaggcttcg ttggattcga
      841 tcaggtcgag gtcggcgacc gtccagccgg ccttttccag ggccttcttg ctggccggga
      901 tcggacccgt gcccatgatt tccggctcga cgccggcgtt tgcccacgag gcgatgcggg
      961 ccagcggggt caggccgcgc ttcttggctt cgtcagcgct catcagcacc agggcggcgg
     1021 cgccgtcatt gaggcccgac gcattggcgg cggtgacgga gccttccttg ttgaaggccg
     1081 gacgcaggcc cgaaatggcg tcgatcgtca cgccgtggcg gatgaactcg tccttgtcga
     1141 cgatggtgtc gcccttgcgg cccttgatgg tgaccggcac gatttcggcg tcgaacttgc
     1201 cggccttctg cgccgcttcg gccttgttct ggctggcgac ggcgaagctg tcctggtcgc
     1261 cgcgcgtgat ctgcca
//
LOCUS       DAOMEO010001091         1275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10706_length_1275_cov_2.662295, whole genome shotgun sequence.
ACCESSION   DAOMEO010001091 DAOMEO010000000
VERSION     DAOMEO010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10706_length_1275_cov_2.662295"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLH31_18700"
     CDS             <1..699
                     /locus_tag="PLH31_18700"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQR91258.1"
                     /translation="PYTHSGVLASALAMDKAKAKAVLAAAGVTVPGGGLFSRHDVARD
                     HVMQPPYVVKPNAEGSSVGVFIVLEGANRPPVELSAPTWTYGEEVMVEPYVRGLELAV
                     AVLGESNGPRALAVTDIRSSTGFYDYDAKYSEGGSIHVLPAPIPDSVRDRAMRMAELG
                     HTALGCQGVTRSDFRYDDINDLLVLLEVNTQPGMTPTSLAPEQADHVGISFDQLVLWI
                     VEDAYARCSAGGIA"
     gene            668..>1275
                     /locus_tag="PLH31_18705"
     CDS             668..>1275
                     /locus_tag="PLH31_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQR91259.1"
                     /translation="MPAVVRGGSPKPRRPRAEAPASPNKARSAAPKGAQPAAKLRAAK
                     GVGLSPAFALGVAGAALGLGLVVMLATGHRAERLVTAARQGVDSEFATAGFKLKTLHV
                     QGASAQADADIRRAAGLYLDQPTLGLDLAEIRQRVEAVGWVKQAKVVRLLPDTVVIAV
                     EERPALAVWQSGGRMRVIDGEGRVISEADAARFPQLPLVVGQG"
BASE COUNT          196 a          416 c          434 g          229 t
ORIGIN      
        1 ccctacaccc actcgggcgt tctggcctcg gcgctcgcca tggacaaggc caaggccaag
       61 gccgtgctgg ccgctgcggg tgtcaccgtg cccggcggcg ggcttttcag ccgccacgac
      121 gtcgcacgcg accatgtgat gcagccaccc tacgtggtga agcccaatgc cgagggatct
      181 tcggttgggg tgttcatcgt tctggaaggc gccaaccgtc cgccggtgga attgtctgct
      241 cccacatgga cttacggcga ggaggtcatg gtcgagccct atgtgcgcgg gctggagctg
      301 gcggtcgccg tgctgggtga gtcaaatggt cccagggccc tggcggtgac cgatattcgc
      361 tcgtccacag gtttctatga ctacgacgcc aagtactcgg aaggcggttc catccacgtg
      421 ctgccggccc caatccctga cagcgtaagg gatagggcca tgcggatggc cgagcttggc
      481 cacacagctc ttggttgcca aggcgtaacc cgatctgact tccgttatga cgacattaac
      541 gaccttctgg tccttctaga ggtcaatacg caacccggca tgacaccgac ttcgctcgca
      601 cccgagcagg ccgaccacgt tggaatttcg tttgatcaac tagtgctctg gatcgtggag
      661 gacgcttatg cccgctgtag tgcggggggg atcgcctaag cctaggcgcc ctcgggccga
      721 agcgcccgca agtccgaaca aggctcgttc ggcggcgccc aagggggcgc agccggccgc
      781 taaactgcgc gccgccaagg gcgtgggctt gtcgcccgcg ttcgccttgg gggtcgccgg
      841 cgcggcgctt ggccttgggc ttgtggtgat gctcgcgacc ggtcatcggg ccgagcgcct
      901 cgtcacggct gcgcgccagg gcgtggacag cgaattcgcg accgccggct tcaagctgaa
      961 gaccctgcac gttcagggcg cgtccgctca ggccgacgcc gatattcgcc gtgccgcggg
     1021 cctctatctc gatcagccca ccctgggcct cgacctcgcc gagatccgcc aacgggtcga
     1081 ggctgtgggc tgggtcaagc aagccaaggt cgtgcgcctg ctgcccgaca ccgtggtgat
     1141 cgccgttgag gagcgccccg cgctggccgt ctggcagagc ggcggccgca tgcgggtcat
     1201 cgacggcgaa ggccgggtga tctccgaggc cgacgccgcg cgtttcccgc aattgccgct
     1261 ggtggtgggg caggg
//
LOCUS       DAOMEO010001092         1272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10742_length_1272_cov_2.163517, whole genome shotgun sequence.
ACCESSION   DAOMEO010001092 DAOMEO010000000
VERSION     DAOMEO010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10742_length_1272_cov_2.163517"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLH31_18710"
     CDS             <1..445
                     /locus_tag="PLH31_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQR91260.1"
                     /translation="EALHDTNRCRALFATHYHELATLEERLDHVSNLSLRAKEWNGDL
                     VFLHEAAPGPADRSYGVQVAKLAGVPAPVVARAREVLDRLETKDESPAKLDDLPLFAM
                     SQAMQAPTKPAPSVIDTALKDLDVDGMSPREALEALYRLKGLLKT"
     gene            526..>1272
                     /locus_tag="PLH31_18715"
     CDS             526..>1272
                     /locus_tag="PLH31_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HQR91261.1"
                     /translation="MPRRLRPTPLEHVVDGHALRARLSAAALDSLGDEAAQRARAIEI
                     LKQALFRGRMIAKERLENGAGGLETSRLLSGVTDEVITALYDFTTVHVFRARNPTEGE
                     RLCLLAVGGYGRRTLAPFSDIDLLFLRPYKTTPHAESVIEFMLYALWDLGFKVGHASR
                     TIEECVRLSREDFTIRTTILEARRLTGDERLAADLKKRFQDEVMKGTGAQFVAAKLKE
                     RDDRQARAGASRYMVEPNVKEGKGGLRDLHT"
BASE COUNT          216 a          449 c          407 g          200 t
ORIGIN      
        1 cgaggccctg cacgacacca accgctgccg cgccctgttc gccacccact atcacgagct
       61 ggcgaccctg gaggagcggc tggaccacgt ctcgaacctg tccctgcggg ccaaggagtg
      121 gaacggcgat ctggtgttcc tgcacgaggc cgcgcccggt ccggccgacc gctcctacgg
      181 cgtgcaggtg gccaagctgg ccggtgtgcc ggcgcctgtc gtggcgcggg cccgcgaggt
      241 gctggatcgc ctggagacca aggacgaatc accggccaag cttgacgacc tgccgctgtt
      301 tgcgatgagc caggccatgc aggcgcccac gaagccggcg cccagtgtca tcgatacggc
      361 gctcaaggac ctggatgtcg acggaatgtc cccgcgcgaa gccttggagg cgctatatcg
      421 ccttaagggt ctcctcaaga cctgactttt atagtgaacg cgtgatggtc gccaaaaccg
      481 gcctccactt ttgactatca cgcttgggaa aaccatatcc ccatcatgcc ccgtcgcctt
      541 cgccccaccc cgctggaaca tgtcgtcgac ggtcacgccc tgcgtgcgcg tctgtcggcc
      601 gccgccctcg acagtctcgg cgatgaggcc gcccagcgcg cccgcgccat cgagatcctg
      661 aaacaggctc tgtttcgcgg ccggatgatc gccaaggagc ggctggagaa cggggccggg
      721 ggcctggaaa cctcgcgcct gctcagcggc gtcaccgacg aggtgattac cgccctctac
      781 gatttcacca ccgtccacgt attccgggcc cgcaacccga ccgagggcga gcgcctgtgt
      841 ctgctggccg tgggcggcta tggccggcgg actctcgcgc cgttcagcga catcgatctg
      901 ctgttcctgc ggccttacaa gaccacgccc cacgccgaga gcgtgatcga gttcatgctc
      961 tatgcgctgt gggacctggg cttcaaggtc ggccacgcct cgcgcacgat cgaggaatgc
     1021 gtgcgcctgt cgcgcgaaga cttcaccatc cgcaccacca tcctggaggc ccgccgcctg
     1081 acgggcgacg agcgcctggc cgccgacctg aagaagcgct tccaggacga ggtcatgaag
     1141 ggcaccggcg cccagttcgt ggctgccaag ctgaaggagc gcgacgaccg ccaggcccgg
     1201 gccggggcca gccgctacat ggtcgagccc aacgtcaagg agggcaaggg cggcttgcgc
     1261 gacctgcaca cc
//
LOCUS       DAOMEO010001093         1271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10752_length_1271_cov_3.137336, whole genome shotgun sequence.
ACCESSION   DAOMEO010001093 DAOMEO010000000
VERSION     DAOMEO010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10752_length_1271_cov_3.137336"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLH31_18720"
     CDS             complement(<1..863)
                     /locus_tag="PLH31_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HQR91262.1"
                     /translation="MSSFEAQALLLDDPRVHPAEDALQQLGHALMNEVLDVFSETALE
                     DFQSTICESLIGAFHSAAQRIEREADKARDDLNRFARDFDGSEIADTEMQAATQKARA
                     ADVATLAIEMVRDAAAASYTTSTGDVWSPWKGSAKASRTTAAQIEAREAIRSAKARKH
                     SATDPGAIIVAFRAAPQADTADDANRIFDALNWALVQWPQMALATTGAKGGEKIAIKW
                     AKQKNVTLVLAKADFDRHGRAAPFRANDELIALEPVCVLTLTNTLNPERGAGMQPFGP
                     ALNLGQKAGEKG"
BASE COUNT          194 a          396 c          452 g          229 t
ORIGIN      
        1 cccttctcgc cggccttttg gccgagattg agggccggtc cgaaaggctg cattccagcg
       61 ccccgttcgg gattgagtgt gttcgtcagg gtgagcacgc acaccggttc gagggcgatc
      121 agttcgtcgt tggcccggaa cggcgccgca cggccgtgtc ggtcgaaatc agccttggcg
      181 agcaccagcg tcacgttctt ctgcttggcc cacttgatgg cgatcttctc accgcccttg
      241 gcgcccgtgg tggccagcgc catctgcggc cattggacga gagcccagtt cagggcgtcg
      301 aagatgcggt tcgcgtcgtc ggcggtatcg gcctgggggg ctgcacggaa ggccacgatg
      361 atcgcgccgg ggtcggtcgc ggaatgcttg cgggccttgg cggaacggat ggcttcacga
      421 gcctcgatct gggcggccgt agtgcgcgaa gccttcgcag aacccttcca ggggctccag
      481 acgtcgccgg tcgatgtcgt atagctggcg gccgctgcgt ctcggaccat ctcaatggcc
      541 agggtggcga catcggcggc gcgggccttt tgggtggcgg cctgcatctc ggtgtcggcg
      601 atctcggagc cgtcgaagtc gcgggcgaag cggttgagat catcccgggc cttgtcggct
      661 tcacgctcga tgcgctgggc tgcggaatgg aaggcgccga tcagggactc gcagatggtc
      721 gactgaaaat cctccagggc agtctcggag aagacgtcga gcacttcgtt catcagggcg
      781 tggccgagct gttggagagc gtcttctgcg gggtggactc gggggtcgtc gagcagcagg
      841 gcctgggcct cgaagctgct catggcgcca gttacgggag agaaggctgt aagttgcatt
      901 gggagaaatc ccttttcgga gtggggtgcg gagcccatcg gctcgttgac cgtctcgcgc
      961 cgacggccgg cgtcccccac aagggctttg agcgcagcgt cccttgtggt gggcgccggg
     1021 cggtggcgag gtcacaacaa gccagagggg ttcgggccct catcgacagg acggatccac
     1081 agcctcttgc actcgctccc gcatcccagg cgccgtgggc tcggggtcca ggtcctgcga
     1141 tgacccgccc actcccagac gcgccaaccc ccgggcctcg ccgaacgaag cgcgggcctc
     1201 cccaagattg aaggggggtc agccgacacc gagaacactg cgcctctccg ccgtcagcac
     1261 aggctgaagc c
//
LOCUS       DAOMEO010001094         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10869_length_1261_cov_2.721393, whole genome shotgun sequence.
ACCESSION   DAOMEO010001094 DAOMEO010000000
VERSION     DAOMEO010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10869_length_1261_cov_2.721393"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="panC"
                     /locus_tag="PLH31_18725"
     CDS             complement(<1..667)
                     /gene="panC"
                     /locus_tag="PLH31_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQR91263.1"
                     /translation="MPQASPTIVRTVAELRAQVGAWKAAGDPVALIPTMGALHEGHLT
                     LVKLGQTRARRTVASVFVNPRQFAPHEDFDAYPRGEAADAEKLASAGCDLMFAPSAAE
                     MYAPGFSTAISVSGVSEPLEGAARPQFFGGVATVVAKLLIQCQADYAVFGEKDYQQLQ
                     VIKRLVRDLDLPVEIISAPTARAEDGLALSSRNAYLSADERQIAAALPKALRSAAQAV
                     AEGG"
     gene            872..>1261
                     /locus_tag="PLH31_18730"
     CDS             872..>1261
                     /locus_tag="PLH31_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HQR91264.1"
                     /translation="MADTRVIVVGNEKGGAGKSTIAVHLVTALLYGGAKVAVIDLDLR
                     QCTAMRFFENRRAWLENKKLPLPEPIQLRLSEDDIALAAAPESEQIERFETAFARGLE
                     EADFVLIDTPGGDTAISRLAHGRADLIV"
BASE COUNT          216 a          418 c          440 g          187 t
ORIGIN      
        1 gtccgccctc tgccaccgct tgggccgcgg aacgcagcgc cttgggcaag gcggcggcaa
       61 tctggcgctc gtcagctgaa aggtaggcgt tgcgcgacga cagggccagg ccgtcctcgg
      121 cgcgcgcggt aggggcggag atgatctcga cgggcagatc caggtcgcgg accagccgct
      181 tgatgacctg cagctgctga tagtccttct cgccaaacac ggcgtaatcg gcctgacact
      241 ggatcagcag cttggcgacc acagtggcga caccgccaaa gaactgcggc cgggcagcgc
      301 cctccagcgg ttcggaaacg ccagagacac tgatcgcggt cgaaaagccc ggggcgtaca
      361 tctcggccgc cgagggtgcg aacatcaggt cgcagccggc cgaggccagt ttttcggcgt
      421 cagcggcctc gccgcgcgga taggcgtcga aatcctcgtg cggggcgaac tgccggggat
      481 tgacgaacac gctggccacg gtgcgccgcg cccgggtctg acccagcttg accagggtca
      541 ggtgcccctc gtgcagggcg cccatggtgg ggatcagggc gaccgggtct ccggcggcct
      601 tccaggcgcc gacctgggcg cgcagctcgg cgacggtccg gacgatagtc ggggaagctt
      661 ggggcatcga cggcctcctg tgcaaacagg cgttggctta tggccgaggg gcgaggcggc
      721 gtctactgcg ccgcacaaga cgtttcaggc cctggttccc cctgcgacaa aagggcctgt
      781 ggaacaaagc gatgacctct cggctgagtc attgcgtgca tgttaacctt tcaaggaccg
      841 tgcgagtcgc ggcgaatgaa ggagcgggtt catggccgat acgcgcgtga tcgtcgtcgg
      901 gaacgaaaag ggcggggcgg gcaagtccac catcgccgtg cacctcgtga cggccctgct
      961 gtacggcggc gccaaggtgg cggtgatcga cctggacctg cgccagtgca ccgcgatgcg
     1021 cttcttcgag aaccgccgcg cctggctgga aaacaagaag ctgccgctgc ccgagccgat
     1081 ccagcttcgc ctcagcgagg acgacatcgc cctggccgcc gcgcccgaga gcgagcagat
     1141 cgagcgcttc gagaccgcct tcgcccgcgg cctggaagag gccgacttcg tcctgatcga
     1201 cacccccggc ggcgacaccg ccatctcgcg cctggcccat ggccgcgccg acctgatcgt
     1261 c
//
LOCUS       DAOMEO010001095         1261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10871_length_1261_cov_2.488391, whole genome shotgun sequence.
ACCESSION   DAOMEO010001095 DAOMEO010000000
VERSION     DAOMEO010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_1261_cov_2.488391"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>1261)
                     /locus_tag="PLH31_18735"
     CDS             complement(44..>1261)
                     /locus_tag="PLH31_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91265.1"
                     /translation="GVARDVTDRVAAERELIEAKAAAEAASRAKSEFLANMSHEIRTP
                     LNGVIGIIDALSRTPLSAEQTEMVNLVRDSGVTLERLVSDFLDVSKIEAGQLQLEVRP
                     FDLEQTLRPCIEVMRYRAEDKGLTFTVERQPCARGRFLGDGTRLGQIIGNLLSNAVKF
                     TETGGVTLRVDMVETPDHALLTLTVEDTGVGFDAEHARRLFSRFSQADETISRRFGGT
                     GLGLSICRSLAQMMGGEISAASEPGVGSRFVVSLPLPREQTLADYDARDSQVAPATAD
                     LHGRPLRVLLAEDHPTNQRVVQLILASQGAELVTVADGIEAVGAFADGAFDVVLMDMQ
                     MPRMDGLTATRAIRALEAAKGAAPTPIIMLSANAMAEHRGEALAAGADLHVAKPITAA
                     NLLAGIQTAIGAV"
BASE COUNT          188 a          426 c          448 g          199 t
ORIGIN      
        1 cgggccgctg ctgtggttca agcgcaagcg ctggctgtag gcgtcaaacc gccccgatcg
       61 ccgtctgaat ccccgccaga agattggcgg cggtgatcgg cttggccaca tgcaggtcgg
      121 cgccggcggc cagggcctcg ccccggtgtt cggccatggc gttggcgctg agcatgatga
      181 tcggcgtcgg ggccgcgccc ttggcggcct ccagggcgcg gatggcgcgg gtggcggtca
      241 ggccgtccat gcgcggcatc tgcatgtcca tcagcaccac gtcgaacgcg ccatcggcaa
      301 aggcccccac cgcctcgatc ccgtcggcca ccgtgacaag ctcggcgccc tggctggcca
      361 ggatcagctg gaccacgcgc tggttggtgg ggtgatcctc ggccagcagc acgcgcaggg
      421 ggcggccatg gaggtcggcc gtggcgggcg cgacctggct gtcgcgcgcg tcgtagtcgg
      481 ccagggtctg ttcgcggggc aggggcaggg aaaccacgaa gcggctgcct acgccgggct
      541 cggacgccgc ggaaatctct ccgcccatca tctgggccaa cgagcggcag atcgacagcc
      601 ccagaccggt gccgccaaac cggcggctga tggtctcatc ggcctggctg aagcgactga
      661 acaggcggcg ggcatgctcg gcgtcgaagc ccacgcctgt gtcctcgact gtcagggtca
      721 gaagcgcatg gtccggggtc tcgaccatgt cgacacgcag cgtgacgccg ccggtctcgg
      781 tgaacttgac ggcgttggac agcaggttgc cgataatctg gccaaggcgc gtgccatcgc
      841 ccaggaaccg gccccgcgcg cagggctggc gctcgaccgt gaaggtgagc cccttgtctt
      901 cggcgcgata gcgcatgacc tcgatacagg gacgcagggt ctgttccaga tcgaacggcc
      961 tgacttccag ctgcagctgg ccagcctcga tcttggagac gtccaggaag tctgacacca
     1021 gccgctccag cgtcacgccg gagtcgcgca ccaggttgac catctcggtc tgctcggcgc
     1081 tgagcggcgt gcgcgacagg gcgtcgataa tgccgatgac gccgttcagc ggggtgcgga
     1141 tttcgtggct catattggcc aggaactcgg acttggcgcg actggccgcc tcggccgccg
     1201 ccttggcctc gatcagttcg cgctcggccg ccacccggtc ggtgacgtcg cgcgccacgc
     1261 c
//
LOCUS       DAOMEO010001096         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10879_length_1260_cov_2.462241, whole genome shotgun sequence.
ACCESSION   DAOMEO010001096 DAOMEO010000000
VERSION     DAOMEO010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10879_length_1260_cov_2.462241"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..788
                     /locus_tag="PLH31_18740"
     CDS             123..788
                     /locus_tag="PLH31_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQR91266.1"
                     /translation="MAQFDLTTPWGRLKTYLNYLWNDHAYLRVGFSNAHWVSPELVRA
                     NQPWPFQLAWWKKRGIRTIVNLRGGFDGSFYALEKDACERLGLNLVDFTITSREVPIR
                     ERVRGAKELFETLDYPALIHCKSGADRAGIMSVLYAHYRLGLPIREAMKELSPRYLHI
                     KAGNTGVLDYVFEQYLERGEPKGLSFTDWVESDDYDPVAMKKTFRAGMLGKLLTDKLL
                     RRE"
     gene            complement(916..1140)
                     /locus_tag="PLH31_18745"
     CDS             complement(916..1140)
                     /locus_tag="PLH31_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HQR91267.1"
                     /translation="MPDKQLLHLVIGGELKDVADIEFRDLSQVEFVGAYASYADAHKA
                     WKAKAQATVDNAHARYFIIHAHKLMDPSLG"
     gene            complement(1171..>1260)
                     /locus_tag="PLH31_18750"
     CDS             complement(1171..>1260)
                     /locus_tag="PLH31_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQR91268.1"
                     /translation="PVPLVPSLVCANATLAPLILNRVGHIDLP"
BASE COUNT          239 a          374 c          423 g          224 t
ORIGIN      
        1 tcggacgtca gggccaagct gtcaagcaca cgggcgcatc accgcgtggg gaaatgatcg
       61 tgcgttttgc attaaacggc ggtccatcct acttaggcgc ggcacttcag aggatcgccc
      121 gtttggccca gttcgacctg accacgcctt gggggcgcct gaagacctat ctcaactatc
      181 tgtggaacga ccacgcgtac ttgcgtgtcg gcttctccaa cgcccactgg gtcagccccg
      241 aactggtgcg cgccaaccag ccctggccgt tccagctggc ctggtggaaa aagcggggga
      301 tcaggaccat cgtcaacctg cgcggcgggt tcgacgggag cttctatgcg ctggagaagg
      361 acgcctgcga gcggctgggt ctgaacctcg tcgacttcac catcacgtcg cgcgaggtcc
      421 cgatccgcga gcgggtgcgc ggcgcgaagg aactgttcga gaccctggac tatccggccc
      481 tgatccactg caagtcgggc gcggaccggg cgggcatcat gagcgtgctc tacgcccact
      541 atcgcctggg cctgccgatc cgcgaggcga tgaaggagct gtcgccgcgc tatctgcaca
      601 tcaaggccgg caataccggc gtgctggact atgtcttcga gcagtatctg gagcgcggcg
      661 agcccaaggg cctgagcttc accgactggg tcgagagcga cgactacgat cccgtggcga
      721 tgaagaagac cttccgggcg gggatgctgg ggaaactgct gactgacaag ctgctgcggc
      781 gggagtaggc gcggaaaaga tgctcccctt ttgggggagc tgtcgcggag cgactgaggg
      841 ggctaatgcg gcataagccg agctggcccc ctccggccct ctgggccacc tcccccaaag
      901 ggggaggatc tgaaatcaac ccaggctcgg atccatcagc ttgtgcgcat ggatgatgaa
      961 gtaccgcgcg tgggcgttgt cgaccgtggc ctgggccttg gccttccagg ccttgtgggc
     1021 gtcggcatag ctggcatagg cgccgacgaa ctcgacctgg gagaggtcgc ggaactcgat
     1081 gtcggcgaca tccttcagtt cgccgccgat gacgagatgg agaagctgct tatcgggcat
     1141 gatggggcac gttcctgcga ggaaatatct ttatggcagg tcgatatgcc cgacccggtt
     1201 aaggatcaat ggcgcaagcg tcgcattggc gcaaaccagg ctcggaacca gcggaacagg
//
LOCUS       DAOMEO010001097         1260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10882_length_1260_cov_2.034855, whole genome shotgun sequence.
ACCESSION   DAOMEO010001097 DAOMEO010000000
VERSION     DAOMEO010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10882_length_1260_cov_2.034855"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..479)
                     /locus_tag="PLH31_18755"
     CDS             complement(75..479)
                     /locus_tag="PLH31_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HQR91269.1"
                     /translation="MNADQKSVAQRCLDSAYAGSRTFPEILNLLHGADIEGYTVDYRR
                     GVTTYYAPDGSSVELANPGVAPGPVEAAFNADIIISGIRDAQTNATGYTYKAFCEKVV
                     AGGCAGYHVSLPGRRAVYFGRTAETHVEHFPA"
     gene            complement(516..971)
                     /locus_tag="PLH31_18760"
     CDS             complement(516..971)
                     /locus_tag="PLH31_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR91270.1"
                     /translation="MTNEISELSDHLGFWLRLVSNHASASFARKLEAMDVTVAEWVMM
                     RELYGQQPMAPSRLAARMGMTRGAISKLADRLLAKRLIARTESSEDGRAHTLNLTAEG
                     RHLTPVLARLADENDEEVFGRLSTEEQRHLRQILRALAERRGLKHPPTA"
BASE COUNT          201 a          398 c          422 g          239 t
ORIGIN      
        1 gaagatcgcg gcctgagcgg tctctggcac tgcgcatccc ctctcccgca cagcggggga
       61 gggttctttt gcggtcaggc cgggaaatgc tcgacgtgcg tttcggccgt gcggccgaaa
      121 tagacggcac gccggccggg aagggagacg tggtagcccg cacagccgcc cgcaacgacc
      181 ttctcgcaga aggccttgta ggtgtagccg gtggcattcg tctgcgcgtc ccggatgccc
      241 gagataatga tatcggcgtt gaacgccgcc tcgaccggac cgggggccac gcccggattc
      301 gccagttcga cgctggaccc gtccggggca taataggttg tgacgcccct ccggtaatcg
      361 acggtgtagc cctcgatgtc cgcgccgtgc aggagattga ggatttcggg aaatgtccgc
      421 gatccggcat aggcgctgtc cagacagcgt tgggcgacgc tcttttgatc ggcgttcatc
      481 agtttctccc cggtcgatgc ggttgctgag ggtggtcagg cggtcggtgg gtgcttcagg
      541 ccgcgccgct ccgcgagggc gcgcaggatc tggcggaggt ggcgttgctc ttccgtcgac
      601 agacgcccga acacctcttc atcattctcg tccgccagcc tcgccaggac cggagtcaga
      661 tgcctaccct ccgcggttag gttcagcgta tgggcgcggc cgtcctcgct gctttcggtc
      721 cgggcgatca ggcgctttgc gaggagacga tccgcaagct tgctgatcgc gccgcgcgtc
      781 attcccatcc tggcggctag gcggctcgga gccatcggct gctgcccgta gagttctcgc
      841 atcatcaccc attcggccac cgtcacgtcc atggcctcca gcttgcgtgc gaagctggct
      901 gacgcatggt tggaaacgag ccgaagccag aacccgagat gatcggacag ctctgaaatc
      961 tcgttggtca tctgccgccc accggtaagg ttgactagga aactataacc atattgtttc
     1021 ctagtcaact agcgggattg atcgacccga agcgccagtg tccgcgcggc agggatcggg
     1081 tgtgaaatgg acgcagggcg cgagccgctg tgccagccac cctccgtccg ccgattgtcg
     1141 cgggcggagg ccggtcagcc ggtcggggtc tgtccctcgc cctgcgcgaa cagggcgtcc
     1201 agcccctcgc gataggtggg gtagcgcagg atcacgccga ggcggtcctt cagcacggcg
//
LOCUS       DAOMEO010001098         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10907_length_1258_cov_2.338321, whole genome shotgun sequence.
ACCESSION   DAOMEO010001098 DAOMEO010000000
VERSION     DAOMEO010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10907_length_1258_cov_2.338321"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /locus_tag="PLH31_18765"
     CDS             complement(<1..>1258)
                     /locus_tag="PLH31_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HQR91271.1"
                     /translation="FMDPPPAAAYGEARSLLQRIQALDDHGDLTTHGKALAEMPLPPR
                     IAHMVVRAAASGQARRGAEVAALLTEQGLGGRETDLRRRLENLGRDRSPRAKDAQALA
                     DRWARAAGKPSGATPLDEGLMLAEAYPERVARARGKPGEFQLAGGRGVYMEPTDPLAR
                     ETWLAVGELGGGEARDRILLAAAVDEVALREAFADRLTAEDRLETGPGGKVRAKRLLR
                     LGKLVLEERLIDKPDPALIASALLAQVRVEGLSALRLGDGVQGLRDRVAFLRGLDGDV
                     WPDLSDAALLERLDQWLEPLLHGRSSLSALDENTLDGAIRSLIPWDAQQKIDAALPAR
                     FKAPTGTTVAIDYAAEAGPRIDIRVQELFGLTEHPSIAGGKIPLVLSLLSPGHKPIQI
                     TRDLPGFWKGSWREVKVEMKGRYLRHI"
BASE COUNT          182 a          456 c          445 g          175 t
ORIGIN      
        1 gatgtgccgg agatagcgcc ccttcatctc gaccttcacc tcgcgccacg agcccttcca
       61 gaaccccggc agatcccgcg tgatctggat cggcttgtga cccggcgaca gcagcgacag
      121 caccagcggg atcttgccgc ccgcgatgct gggatgctcg gtcaggccaa acagttcttg
      181 gacccgaatg tcgatgcgcg gcccggcctc ggcggcgtag tcgatagcga cggtggtgcc
      241 ggtcggggcc ttgaagcggg ccggcagggc cgcgtcgatc ttctgctggg cgtcccacgg
      301 gatcagcgag cggatcgcgc catccagcgt gttctcgtcc agggccgaca gggacgagcg
      361 gccatgtagc aagggctcca gccactgatc cagccgctcc agcaaagcgg cgtccgacag
      421 gtcgggccag acgtcgccgt ccaggccgcg caggaaggcg acgcggtcgc gcagcccctg
      481 aacgccatcg cccagccgca gcgccgatag cccctccact cgaacctgag ccagcaaggc
      541 gctggcgatc agcgccgggt cgggcttgtc gatcagccgc tcctcaagca ccagcttgcc
      601 gaggcgcagc agccgcttgg cgcggacctt gccgccgggg ccggtttcca gtcggtcctc
      661 ggcggtgagg cgatcggcga aggcctcgcg tagggcgact tcgtcgacgg cggcggccag
      721 caggatgcgg tcgcgggcct cgccgccgcc cagttcgccc acggccagcc aggtctcacg
      781 ggccagagga tcggtcggct ccatatagac gccgcgcccg ccggccagct ggaactcgcc
      841 cggcttgccg cgcgcccggg ccacgcgctc gggataggcc tcggccagca tcaatccttc
      901 gtccaaaggt gtggcgccgg acggcttgcc cgccgcccgc gcccagcggt cggccagcgc
      961 ttgggcgtcc ttggcccggg gggagcggtc acggccgagg ttctccagtc gccggcgaag
     1021 atccgtctcg cgaccgccaa ggccctgctc ggtcagcagg gcggcgactt ccgccccacg
     1081 ccgcgcctgc cccgacgcgg cggcgcggac caccatgtgg gcgatgcgcg gcggcagcgg
     1141 catctcggcc agggccttgc catgggtggt gaggtcgcca tggtcgtcga gagcctgaat
     1201 ccgctgcagc aatgatcgcg cctcgccata ggcggcggcg ggcggcggat ccatgaag
//
LOCUS       DAOMEO010001099         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10916_length_1257_cov_2.336938, whole genome shotgun sequence.
ACCESSION   DAOMEO010001099 DAOMEO010000000
VERSION     DAOMEO010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10916_length_1257_cov_2.336938"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1257
                     /locus_tag="PLH31_18770"
     CDS             <1..>1257
                     /locus_tag="PLH31_18770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HQR91272.1"
                     /translation="GTKGRNARETARYINAAQKWAREKTRLGIPLLMHDEALHGYVAR
                     DATSYPQSIALASSFDPELVERVFSMAAREMRARGSNMALAPVVDVARDPRWGRIEET
                     YGEDPHVCAEIGLAAIRGFQGKTLPLAPDKVFVTLKHMTGHGQPENGTNVGPAQIAER
                     TLRENFFPPFERAVTELPVRSVMPSYNEIDGVPSHASRWLLTDILRTEWGYQGSVQSD
                     YFAIKELANRHKMTDSLADTAVMAMNAGVDVELPDGEAYALLPQLVKEGRVSMAQIDA
                     AVARVLTMKFEGGFFENPYADEKTAEAKNNTPEAIALAREAARKSVVLLKNDKGVLPL
                     NGKAIKRMALLGTHARDTPIGGYSDEPKHVVSLLEGLTAEAKAQGFALDYAEAVRITE
                     RRVWAADEVKLVDPAVNARLIAEAVE"
BASE COUNT          233 a          441 c          407 g          176 t
ORIGIN      
        1 acggcaccaa ggggcgcaac gcccgggaaa ccgcccgcta catcaacgcc gcccaaaagt
       61 gggccaggga aaagacccgc ctgggcatcc ccctgctgat gcatgacgag gccctgcacg
      121 gctatgtggc tcgcgacgcc accagctatc cgcagtcgat cgccctggcc tccagcttcg
      181 atcccgagct ggtcgagagg gtgttctcca tggccgccag ggagatgcgc gcgcgcggct
      241 ccaacatggc cctggcgccc gtggtcgacg tggcccgcga cccgcgctgg ggccggatcg
      301 aggagaccta tggcgaagac ccgcacgtct gcgccgagat cggcctggcc gccattcgcg
      361 gcttccaggg caagaccctg ccgctggccc ccgacaaggt gttcgtcacc ctcaagcaca
      421 tgaccggcca tggtcagccc gaaaacggca ccaatgtggg cccggcccag atcgccgaac
      481 gcaccctgcg cgagaacttc ttcccgccct tcgagcgcgc ggtgaccgag ctgccggtgc
      541 ggtcggtgat gccgtcctac aacgagatcg atggggtgcc ctcgcacgcc agccgctggc
      601 tgctgaccga catcctgcgc accgagtggg gctatcaggg ctcggtccag agcgactatt
      661 tcgcgatcaa ggaattggcc aaccgccaca agatgaccga cagcctggcc gacaccgccg
      721 tgatggcgat gaacgccggc gtcgatgtcg agctgcccga cggcgaggcc tatgccctgc
      781 tgccgcagct ggtgaaggaa ggccgcgtct cgatggccca gatcgacgcc gccgtggccc
      841 gcgtcctgac catgaagttc gagggcggct tcttcgaaaa cccctatgcc gacgagaaga
      901 ccgccgaggc caagaacaat acgccagaag ccatcgccct ggcccgtgaa gccgcccgca
      961 agtcggtggt tctgctgaag aacgacaagg gcgtgctgcc gctgaacggc aaggcgatca
     1021 agcgcatggc gctgctaggc acccacgccc gcgacacccc gatcggcggc tattcggacg
     1081 agcccaagca tgtggtctcg ctgctggaag gcctgaccgc cgaggccaag gctcagggct
     1141 ttgctctgga ctacgccgag gctgtgcgga tcaccgaacg ccgcgtctgg gccgccgacg
     1201 aggtcaagct ggtcgacccg gccgtcaacg ccaggctgat cgccgaagcc gtcgagg
//
LOCUS       DAOMEO010001100         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10917_length_1257_cov_2.205491, whole genome shotgun sequence.
ACCESSION   DAOMEO010001100 DAOMEO010000000
VERSION     DAOMEO010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10917_length_1257_cov_2.205491"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLH31_18775"
     CDS             complement(<1..797)
                     /locus_tag="PLH31_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR91273.1"
                     /translation="MHDHLEHTAIRTDLGAIFVSLELSRSIWLITSLSPGAGEKLSRH
                     SVPAGDVGGLLARLAQLKHKAADRLDREFPFIVIQEAGLDGFWIHRVLESEGIESHVV
                     DAASVLVSRRRRQAKTDRIDGETLVRTLLAYKRGEPRVCSMVRPPSPEEEDRRRICRE
                     RKTLLAERIKHTNRIKGLLCAQGIFDYDPLLRNRRARLDGLQTGDGRTLPARLKAQIG
                     RELDRIELLLDQLKAVEVERDALIQPAEETAPTPAVMLAQLKGIGSQ"
BASE COUNT          217 a          384 c          407 g          249 t
ORIGIN      
        1 atctgcgagc ctatgccttt caactgcgcc agcatcaccg ccggcgtcgg cgcggtctcc
       61 tcggcaggct ggatgagagc atcgcgctcg acctcgacgg ccttcagctg atccaacagc
      121 aattcgatcc gatccagttc ccggccgatc tgcgccttta gccgcgcggg caaggttcga
      181 ccgtcaccgg tctgtaggcc gtccagtcgg gcacgccggt tccgaaggag tggatcatag
      241 tcgaagatgc cctgtgcaca gagcagtccc ttgatcctgt tcgtgtgctt gatgcgctcg
      301 gcgagcagcg tcttccgctc acgacagatc cgccggcgat cttcctcctc gggggagggc
      361 ggtcggacca ttgaacaaac ccggggctcg ccgcgcttgt aggccaaaag cgtacgcacc
      421 agcgtctcgc cgtcgatccg atccgtcttg gcttggcgcc gccggcgcga caccaggacc
      481 gacgcggcat cgacgacatg gctttcaatg ccttcgctct ccaggacccg atggatccag
      541 aacccgtcaa gaccggcctc ttggatgacg atgaagggga attccctatc tagacggtcg
      601 gccgctttgt gcttgagctg agcgagccgg gccagcaaac caccgacgtc gcctgcggga
      661 actgagtgcc gggatagttt ttcgcctgcg ccaggcgaga gggaggtgat cagccagatc
      721 gatcggctga gctccagcga gacaaagatc gcgccgaggt cggtgcggat ggcggtgtgt
      781 tctaggtggt catgcatcgg gcgagctccg gcgggttgga catagcgccc tcactctgcc
      841 ggtggccgag cggagcgcta ccccgcccca tgggatcttc atttttgctt tgagtatttc
      901 gcgaccaccc gccctcggag ggaaaaatcg aacatgggta ttgtcgggcg agtgggcgtt
      961 gttagtctgc aacctgctgt tggggtgagg ttgggtccgt gaaggctgat gagtttaaga
     1021 cctggcttca ggccgccgct ggcgtggtca gcccttaacc gccaaggcca ttcaacaccg
     1081 ccgggggagg ctcaagcggg tcgagcgctc gctggccgcc atgggatttc ctgacgcgga
     1141 tattgatgaa ctgatttcgc ggggggaact gcccgccatg ctcgccaagt tgagcagcta
     1201 tattggctcc cgcgagggcg cgccgccggt aagtcttgtc cctcaggcgg agaatcc
//
LOCUS       DAOMEO010001101         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10919_length_1257_cov_1.931780, whole genome shotgun sequence.
ACCESSION   DAOMEO010001101 DAOMEO010000000
VERSION     DAOMEO010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10919_length_1257_cov_1.931780"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>334)
                     /locus_tag="PLH31_18780"
     CDS             complement(<1..>334)
                     /locus_tag="PLH31_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HQR91274.1"
                     /translation="VAPQPVAEVVEFTPPTPAPVAEPTLFETATRTSPSVFNLDGFST
                     SEDVALSALDVPFTEPEAPRFGNLSLLVSLGVLGLAMFGGGVLWILTVASGPGFSQIK
                     MFGYGASMI"
     assembly_gap    335..344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<345..1227)
                     /gene="spmX"
                     /locus_tag="PLH31_18785"
     CDS             complement(<345..1227)
                     /gene="spmX"
                     /locus_tag="PLH31_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-family localization factor SpmX"
                     /protein_id="HQR91275.1"
                     /translation="MKPRHQVSRAAVDLIKRFEGYRMKAAQLPDGRWTIGYGHTLTAR
                     GGATVSEKDAEALLLYDLISVAHAVNEQTYAPLNQNQFDALVCFAFNIGVDNFMRSGV
                     LRRINEGSLLQAACAMEMWRKADFEGERIVIDALVRRRSAEKALFLTPADGVWIAAPS
                     PVLRPKVDYDASCSVPKQTPAAVMTRFEGDRAVVERKEGAPLVIVVPDEEGPSASEQA
                     AAAVSARLEAILADNPVPVAPTPTPTPTPAPTPVRDDLGLPEPPVPAAAPPAAVLYED
                     FEETVSAPFRLTPEPAAP"
BASE COUNT          215 a          396 c          448 g          188 t
ORIGIN      
        1 gatcatgctg gcgccatagc cgaacatctt gatctgcgag aagcccggac ccgaggcgac
       61 ggtgaggatc cacaggaccc cgccgccaaa catcgccagg cccagcacgc ccaggctgac
      121 cagcaggctg agattgccga aacgcggggc ttccggctcg gtaaacggaa cgtccagggc
      181 gctgagggcc acgtcttcgc tggtcgagaa gccgtcgaga ttgaacaccg agggcgaagt
      241 ccgcgtcgcg gtctcgaaca gggtgggctc ggcgacggga gccggcgtcg gcggggtgaa
      301 ttcaacgacc tcggcaaccg gctgcggcgc gaccnnnnnn nnnnccgggg ctgcaggctc
      361 cggcgtcaga cggaacggcg cgctgacggt ctcctcgaag tcctcataga gcacggcggc
      421 gggcggcgcg gcggccggta ccggcggttc cggtagtccc agatcgtcgc gaaccggggt
      481 cggagccggc gtcggcgtgg gcgtcggcgt cggcgcgacc ggaacaggat tgtcggcgag
      541 gatcgcctcc agacgcgcgc tcacggcggc ggcagcctgc tcgctggcgc tggggccttc
      601 ttcgtcagga acgacgatga ccagaggcgc gccctccttc cgttcgacca cagcgcggtc
      661 gccctcgaag cgggtcatca ccgcggccgg cgtctgcttg ggcacagagc agctggcgtc
      721 gtagtcgacc ttgggccgca ggacggggct gggcgcggcg atccacacgc catcggccgg
      781 cgtcaggaac agggccttct cggccgagcg gcgacggacc agggcgtcga tgacgatacg
      841 ctcgccctcg aagtcggcct tgcgccacat ctccatcgcg caggcggcct gcagcagcga
      901 gccttcattg atccggcgca ggacgcccga gcgcatgaag ttgtcgacgc cgatattgaa
      961 ggcgaaacag accagcgcat cgaactggtt ctgattgagg ggcgcgtagg tctgttcgtt
     1021 gaccgcatgg gccaccgaga tcagatcgta gagcaggagg gcttcggcgt ccttttccga
     1081 aacggtcgcc ccaccgcggg cggtgagggt atggccgtag ccgatggtcc agcgcccatc
     1141 gggcagctga gcggctttca tccggtagcc ttcgaatctc ttgatgagat cgacggcggc
     1201 gcgggagacc tgatgacgcg gtttcatcgc taggaatgac ccagtttgag acagacg
//
LOCUS       DAOMEO010001102         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10923_length_1257_cov_1.709651, whole genome shotgun sequence.
ACCESSION   DAOMEO010001102 DAOMEO010000000
VERSION     DAOMEO010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10923_length_1257_cov_1.709651"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLH31_18790"
     CDS             complement(<1..249)
                     /locus_tag="PLH31_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91276.1"
                     /translation="MATSTEMKIALLLSSALTLLAVIPATAGQPPRPARDPDIAISHR
                     DRVYAAEQFSNTVSVTDPVDNRLLGFIKLGDPAPGNLSP"
     gene            complement(233..658)
                     /locus_tag="PLH31_18795"
     CDS             complement(233..658)
                     /locus_tag="PLH31_18795"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HQR91277.1"
                     /translation="MSLRQTPSKRLGGWSLALAAALAATPLAASTPHSEFMSANDAAM
                     IKMMADMDIKPSGDVDRDFVNMMIPHHQGGIDMAIAQLRYGKNEQLRRLAQEIIVAQQ
                     QEIAAMRLAVGQPLPPSSPSPTTPARHGSTSAAHHGHEH"
     gene            828..1226
                     /locus_tag="PLH31_18800"
     CDS             828..1226
                     /locus_tag="PLH31_18800"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91278.1"
                     /translation="MPRPVILGTLVGLCLVAAESAGAQTPAPPLKIGQTIKGPSLVDE
                     ADMTLYVFANDTRGKSACNDQCARNWPPLEAKVAAVAIGDYAPITRDDGSKQWAFKGR
                     ALYRFARDQAPGDIKGDGLLNGAWSIAKPK"
BASE COUNT          218 a          399 c          429 g          211 t
ORIGIN      
        1 cggactgaga ttgcccggcg ccggatcgcc cagcttgatg aagcccagga gacggttgtc
       61 gacgggatcg gtgaccgaga cggtgttgga gaattgctcg gcggcataga cgcgatcgcg
      121 atgcgagatg gcgatgtccg ggtcgcgcgc gggcctgggc ggctgaccgg cggttgcggg
      181 aatgacggcc aagagcgtca gggccgagct cagcaggagg gcgatcttca tctcagtgct
      241 cgtggccatg gtgcgcagcg ctcgtcgagc cgtgacgcgc tggcgtcgtc ggcgagggac
      301 tggagggcgg cagaggctgg cccacggcca ggcgcatggc cgcgatctcc tgctgctggg
      361 cgacgatgat ctcctgggcc agccggcgca gctgctcgtt cttgccatag cgcagctggg
      421 cgatggccat gtcgatgccg ccctgatggt gggggatcat catgttcacg aagtcgcggt
      481 ccacgtcgcc tgagggcttg atgtccatat ccgccatcat cttgatcatg gccgcatcat
      541 tggcgctcat gaactcgctg tgcggcgtgc tggccgccag cggcgtggcg gccagcgctg
      601 cggcgagggc cagggaccat cctcccaagc gtttcgaggg cgtttgacgc aaggacatgg
      661 aaggtctccg gacggcgaga cgatcaagcg cctcgatcgt gagacgctct caagacgccg
      721 attattcccg cgacatcgac ggcgacgaaa aaaacgcgcg ctcggaataa tcgtcggtgg
      781 cgggcgtcta acggttcagg tcgatcccga ccgctggagt tcgcaccatg ccaagacccg
      841 tcatccttgg aaccttggtc ggcctgtgcc tcgtcgccgc cgaaagcgcc ggcgcccaga
      901 caccggcgcc gcccctcaag atcggccaga ccatcaaggg gccgtctctg gtcgatgaag
      961 ccgacatgac cctctacgtc ttcgccaacg acacacgcgg caagtcggcg tgcaacgatc
     1021 aatgcgcgcg caactggccg ccgctggagg ccaaggtcgc cgcggtcgct atcggcgact
     1081 atgccccgat cacgcgagac gatgggtcca agcaatgggc cttcaagggc cgcgccctct
     1141 atcgcttcgc ccgggatcag gcgccgggcg atataaaggg tgacggtttg ctgaacggcg
     1201 cctggtcgat cgccaagccc aaatagtctg actaccgggc gcttcgcccc gcctccc
//
LOCUS       DAOMEO010001103         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10960_length_1254_cov_3.824020, whole genome shotgun sequence.
ACCESSION   DAOMEO010001103 DAOMEO010000000
VERSION     DAOMEO010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10960_length_1254_cov_3.824020"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLH31_18805"
     CDS             complement(<1..733)
                     /locus_tag="PLH31_18805"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HQR91279.1"
                     /translation="METDRTGTSVGRFVYGRRYLAHPDAVEIDPVELKLAPRTYETAA
                     LGGVFGALRDASPDYWGRRVIDKHAGKPQLGEIDYLLHSADDRAGALGFGLNAEPPAP
                     RRDFNQTVALEKLQRIADALIADEEVAPDAANQQIQDLLLVGTSMGGARPKAVVEDEG
                     ALWIAKFNRQDDRWNSARVEHAMLTLARACGLEAADSKVVLAGGRDVLMVRRFDRERV
                     AAGYTRARMISALTLLRTEDSHRARD"
     gene            complement(808..1149)
                     /locus_tag="PLH31_18810"
     CDS             complement(808..1149)
                     /locus_tag="PLH31_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91280.1"
                     /translation="MPARNTLLLAPPYPVELALTELGANLRTARLRRNLTVEEVAQKI
                     GAGRRAVSDAERGKPTTSVAIYAALLWAFDLLADMHSLADPARDQEGQVLARSKARIR
                     ARRGEALDNDF"
BASE COUNT          204 a          395 c          434 g          221 t
ORIGIN      
        1 gatcccgcgc ccgatggctg tcctccgtcc gcaggagagt cagggcgctg atcatccggg
       61 cgcgggtgta gcccgccgcc acgcgctcgc ggtcaaagcg gcggaccatc aggacgtctc
      121 gccctcccgc aaggaccacc ttgctgtcgg cggcttccag accgcaggcc cgggcaagcg
      181 tgagcatggc gtgttcgacc cgggcgctgt tccagcggtc atcctggcga ttgaacttgg
      241 cgatccagag agcgccctcg tcctcgacga cggccttggg cctggccccg cccatggagg
      301 tgccgaccag aagcagatcc tggatctgtt ggttggcggc gtccggcgcg acctcttcgt
      361 cggcaatcag ggcgtcggcg atacgctgca gcttctccag cgcgacggtc tggttgaaat
      421 cgcggcgcgg cgccggcggc tccgcattga ggccgaagcc cagcgcgccg gcgcggtcgt
      481 cggcggagtg cagcaggtag tcgatttctc cgagttgcgg cttgccggca tgtttgtcga
      541 tgacgcggcg gccccaatag tcggggctgg cgtcgcgcag ggcgccgaac acgccgccga
      601 gcgcggcggt ctcgtaggtg cggggtgcga gcttcagctc gaccggatcg atttcaacgg
      661 cgtctggatg cgccaggtag cgacggccat agacgaaacg tccgacggac gtgccggtcc
      721 tgtcggtctc catgacaaag cgaccggcgg tgatcggctc aacggcccct ggaagggtga
      781 tatggacaaa ggcctctgcc atcggcctta gaaatcgttg tccaaggctt caccgcgccg
      841 cgcgcgaatc ctggccttgg agcgtgcaag aacctgtcct tcctggtcgc gagctgggtc
      901 ggccaggctg tgcatgtcgg ccagaaggtc gaaggcccaa agcagggcgg catagatcgc
      961 gacactggtt gttggcttgc cacgttcggc gtccgacaca gcgcggcgac ccgctccaat
     1021 cttttgggcg acctcctcga cggtcaggtt tcgacgcagg cgtgctgtcc gcaggttcgc
     1081 cccgagttct gtcagggcaa gttccacggg atagggcggt gcgaggagca gcgtgttgcg
     1141 ggcgggcatg ggcgttcaag agccagttag gcgacctatt gtgcgtcaaa gagcaaacca
     1201 caatttcgca gagcctgcaa ggcgcacgct cttgaacgcg cttaagaccg ctta
//
LOCUS       DAOMEO010001104         1254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_10965_length_1254_cov_2.154295, whole genome shotgun sequence.
ACCESSION   DAOMEO010001104 DAOMEO010000000
VERSION     DAOMEO010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10965_length_1254_cov_2.154295"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..400)
                     /gene="cpdR"
                     /locus_tag="PLH31_18815"
     CDS             complement(44..400)
                     /gene="cpdR"
                     /locus_tag="PLH31_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle two-component system response
                     regulator CpdR"
                     /protein_id="HQR91281.1"
                     /translation="MARILLAEDDDSLRGFLARALERAGFEVQACADGEEAVQHLDHP
                     WDLLLTDIVMPGMDGIEVARQAAARDPDLRIMFITGFAAVALSAQDRAPAGAKVLSKP
                     VHLRDLVAEVEKMMAA"
     gene            565..>1254
                     /locus_tag="PLH31_18820"
     CDS             565..>1254
                     /locus_tag="PLH31_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HQR91282.1"
                     /translation="MSAWEPIGTRNQPASAVASPVDPLACPAHDVLRGAPEPAPPPTA
                     LVFASPHSGSIYPADMMAAARLDAEAIRSSEDAFVDQIIAGAPDLGAAVIRARLARAY
                     IDLNREPWELDPAMFEDELPEYARSRSARVAAGLGAIARVAAEGRPIYARKLTFAEAQ
                     ARVELAHRPYHDALDRLLAAARSAHGCAILLDWHSMPAAAARGHRARGGGPCDIVLGD
                     RFGAACSPKLTA"
BASE COUNT          193 a          441 c          420 g          200 t
ORIGIN      
        1 tatagcggcg gtgtttgcga tgcgcaagcc gcttgtcggc cttttatgcc gccatcatct
       61 tttcgacctc ggcgaccagg tcgcggaggt gaacgggctt tgacagcacc tttgccccgg
      121 ccggcgcgcg atcctgggcc gagagggcca cagcggcgaa gccagtgatg aacatgatgc
      181 gcagatcggg gtcgcgcgcg gccgcctgac gcgccacctc gataccgtcc atgccaggca
      241 tcacgatgtc ggtcagcaga agatcccacg gatgatcaag atgctgaaca gcttcctcgc
      301 catcggcgca ggcctggacc tcgaaccccg cgcgctcaag ggcgcgcgcc aagaaacccc
      361 ggagggagtc gtcgtcttcg gcgagaagga tgcgagccat gtcggtcttt tttccgcaga
      421 tcgtcggcgt tgggaaccca ccctaacccg ccgactgtaa atcggggatg aactctgcgc
      481 actgcgacag gaccgcccaa ctttcccggg gaggtttgtc cgaaggtgtt tgaccccgcc
      541 gggcgcgcct tgcgaatatg gcccatgagc gcttgggagc caatcgggac gagaaaccag
      601 ccggcgagcg ccgttgcgtc gcccgtcgat cccctcgcct gcccggcgca tgatgtcctg
      661 cgcggcgcgc ccgagcccgc gccgccgccg accgctctgg ttttcgcctc gccgcactcg
      721 ggcagcatct atcccgccga catgatggcc gccgcgcgcc tggacgccga ggcgatccgc
      781 agttcggaag acgccttcgt cgaccagatc attgccgggg cgcctgatct cggagcggcg
      841 gtgatccggg cgcggctggc ccgggcctat atcgatctca accgcgagcc ctgggagctg
      901 gacccggcga tgttcgaaga tgaactgccc gaatacgcca gaagccgctc ggcccgggtc
      961 gccgcgggtc tgggcgccat cgcccgggtg gcggccgaag gtcggcccat ctacgcccgc
     1021 aagctgacct tcgccgaggc ccaggcgcgg gtcgagctgg cccaccggcc ctatcacgac
     1081 gccctcgacc gcctgctggc ggcagcgcgg tcggcccatg gctgcgcgat cctcctcgac
     1141 tggcattcga tgccagccgc ggcggcgcgc ggtcacagag cgcggggcgg cggcccctgt
     1201 gatatcgtgc tgggcgaccg gtttggcgcg gcctgctcgc ccaagctgac agcc
//
LOCUS       DAOMEO010001105         1248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11026_length_1248_cov_3.007544, whole genome shotgun sequence.
ACCESSION   DAOMEO010001105 DAOMEO010000000
VERSION     DAOMEO010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11026_length_1248_cov_3.007544"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..>1248)
                     /locus_tag="PLH31_18825"
     CDS             complement(232..>1248)
                     /locus_tag="PLH31_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR91283.1"
                     /translation="HIARAESLSAVESARVLVSSDELPARTRTALSNFLRDLDRWRAL
                     AATTPHQRVLEAVLEESGYTDALRLDKGPTSQTRLENLKELVQSMSAFDTLESYLEHV
                     SLVMDLDKGAGDEAVWVMTLHSAKGLEFPLVFLPGWEEGVFPSQRSMDDKGTKGLEEE
                     RRLAYVGITRAREEARISFVANRQVYGRWTSQLPSRFVDELPLAHVEAASETGYYGGG
                     PGMQQTAASRWDDAGQSSFGGSGYASPGWKRAQDRMAGGGNSWSNPRTGGSGGRSAPI
                     EGEGRLVAVSAPSKASAYQRGDRVFHVKFGYGKVSAVEGNKLSVAFDKAGDKKVIDSF
                     VEKA"
BASE COUNT          189 a          440 c          411 g          208 t
ORIGIN      
        1 gcaaaacaca catcacggtc atccgggttc gccccggccg cttcgcgcct ccccactaag
       61 ctcagcgagt gatcgcgtga ggctgagaac ctgtagctgc tcccctctgg gggagctgtc
      121 gcggagcgac tgagggggcc atcgcagcca tctcacagct agccccctcc ggccctacgg
      181 gccacctccc ccaaaagggg aggctctacg tgacggagtg ctcagatgcg ctcacgcctt
      241 ctcgacgaag ctgtcgatca ccttcttgtc gccggccttg tcgaaggcca ccgacagctt
      301 gttgccttcg acggccgaca ccttgccgta gccgaacttg acgtggaaca cgcggtcgcc
      361 gcgctgatag gcgctggcct tggacggcgc cgagacggcg accagacggc cttcgccctc
      421 gatcggggcc gagcggccgc ccgagccgcc ggtgcgggga ttgctccagg aattgccgcc
      481 gccggccatg cggtcctggg cgcgtttcca gccggggctg gcatagccgg aaccgccgaa
      541 cgagctttga cctgcatcgt cccagcgcga ggccgccgtc tgctgcatgc ccggaccgcc
      601 gccataatag ccagtctcgg acgccgcctc gacatgggcc aggggcagtt cgtcgacaaa
      661 ccgcgagggc agctggctgg tccagcggcc atagacctgc cggttggcca caaagctgat
      721 ccgcgcctct tcgcgggccc gggtgatgcc gacataggcc aggcggcgct cttcctcgag
      781 gcccttggtg cccttgtcgt ccatgctgcg ctggctgggg aagacgcctt cctcccagcc
      841 gggcaggaag accagcggga actccaggcc cttggccgag tgcagggtca tgacccagac
      901 cgcttcgtcg ccggcgccct tgtccaggtc catgaccagg gagacgtgct ccagatagct
      961 ttccagggtg tcgaaggcgc tcatcgactg gacgagttcc ttcaggttct ccagccgcgt
     1021 ctggctggtg ggacccttgt cgaggcgcag ggcgtcggta tagccgctct cttccagcac
     1081 cgcttccagc accctctggt gcggggtggt ggccgccaag gcgcgccagc gatccaggtc
     1141 gcgcaggaag ttgctcagcg ccgtgcgcgt gcgggccggg agctcgtcgc tgctgaccag
     1201 aacgcgcgcc gactccaccg ccgacaggct ctcggcccgg gcgatgtg
//
LOCUS       DAOMEO010001106         1247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11049_length_1247_cov_2.350671, whole genome shotgun sequence.
ACCESSION   DAOMEO010001106 DAOMEO010000000
VERSION     DAOMEO010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_1247_cov_2.350671"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1247)
                     /locus_tag="PLH31_18830"
     CDS             complement(<1..>1247)
                     /locus_tag="PLH31_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQR91284.1"
                     /translation="PSWDCLPYDRVGPSAGVAAQRMATLSRLARGLGSAKPAILVISA
                     PALLQRVPDKSVLMRASYSAKVGGSVDIKDLERYFAVNGYVRASTVSEKGEFAIRGGV
                     IDVYPSAAEEPVRLDLFGDTLESIRAFDPETQRSTRQLKEIDLLPVSEALLDADGISR
                     FRRGYVAKFGAPGDDPLYNTVSEGGRRAGLEHWLPLFYERMATLFDYLPDGALVGVDH
                     QASEARDERLAMIEDAYQARASADRKSAYRPLEPSALYLTAAEWEEALSDRPHRRFTP
                     FQPQGLDVVDMGAKLGRVFAAERAQDSVNLFEATAAHASALAASGKRVLFASWSEGSS
                     ERLGTMLADHGLKKITYAGYWQAAKANDPKVPQRVVLPLDHGFETENLAVISETDILG
                     DRLARPRKKRRAGVPPFANRLRL"
BASE COUNT          202 a          414 c          441 g          190 t
ORIGIN      
        1 gcaatcggag ccggttcgcg aacggtggaa cgccggcgcg gcgcttcttg cgcggcctcg
       61 ccaggcggtc gcccaggatg tcggtctcgg agatgaccgc caggttttcg gtctcaaagc
      121 cgtggtccag cggcaggacc acgcgctgcg ggaccttggg atcattggcc ttggccgcct
      181 gccagtagcc ggcataggtg atcttcttca ggccatggtc ggccagcatg gtccccaggc
      241 gctcggacga gccctccgac catgaggcga acagcacgcg cttgccgctg gccgccagag
      301 cgctggcgtg ggcggccgtg gcctcgaaca gattgacact gtcctgggcg cgctcggcgg
      361 cgaacacccg gcccagcttg gcgcccatgt cgacaacgtc caggccctgg ggctggaacg
      421 gcgtgaaccg gcggtgggga cggtcgctga gcgcctcctc ccactcggcg gcggtcagat
      481 agagggcgct aggctccagc gggcgatagg ccgatttgcg atcggcgctg gcccgggcct
      541 gataggcgtc ctcgatcatc gccaggcgtt cgtcgcgcgc ttcggaggcc tgatgatcga
      601 cgccgaccag ggcgccgtcg ggcagatagt cgaacaaggt cgccatccgc tcgtagaata
      661 gcggcagcca gtgttcgagg ccggcgcggc ggccgccctc ggagacggtg ttgtagagcg
      721 gatcatcgcc cggcgcgccg aacttggcga catagccccg gcgaaagcgc gaaatgccgt
      781 cggcgtcgag caaagcctcg ctgaccggca ggagatcgat ctccttcagt tgcctggtcg
      841 agcgctgggt ttccggatca aaggcgcgga tgctctccag cgtgtcgccg aacaggtcca
      901 gccgcacggg ctcctcggcg gccgagggat agacgtcgat gacaccgccc cggatcgcga
      961 actcgccctt ttccgagaca gtggaggcgc ggacatagcc gttgaccgcg aagtagcgct
     1021 ccagatcctt gatatcgacg ctgccgccga ccttggctga atagctggcg cgcatcagta
     1081 ccgacttgtc aggcacccgc tgcagcaggg ccggcgcgga gatgaccagg atcgccggct
     1141 tggccgagcc aagccctcgg gccagtcgcg acagcgtcgc catgcgctga gccgcgacgc
     1201 ccgccgaggg gccgacccgg tcatagggca ggcagtccca cgacggg
//
LOCUS       DAOMEO010001107         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11067_length_1246_cov_2.829555, whole genome shotgun sequence.
ACCESSION   DAOMEO010001107 DAOMEO010000000
VERSION     DAOMEO010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11067_length_1246_cov_2.829555"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>848
                     /locus_tag="PLH31_18835"
     CDS             171..>848
                     /locus_tag="PLH31_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQR91285.1"
                     /translation="MVNVPEARPAPRPPHIVDTLIAERAPRLTASPLWPLLRRPLYAV
                     LGYDKARRMADTIAGIGGLAALEHVSALLDLKVDVRGLENVPTRGRLVVVGNHPTGIA
                     DGVAVHDALKQVRPDTVFYANADAHRVCPRFDEVLIPVEWVEAKRTRDRMRLTLQMTR
                     QAMEAERALMIFPAGRLAVKDEEGLLSDPPWAASAVSIARKYEAPIIPIHVAGPWSTL
                     FHLFDRRH"
     assembly_gap    849..858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <859..1040
                     /locus_tag="PLH31_18840"
     CDS             <859..1040
                     /locus_tag="PLH31_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR91286.1"
                     /translation="LRDITLFHELLNKTGRRFALIVGRAIDPESLAADTGEATTTLKR
                     YVERTLPKHPEGALT"
     gene            1052..>1246
                     /locus_tag="PLH31_18845"
     CDS             1052..>1246
                     /locus_tag="PLH31_18845"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQR91287.1"
                     /translation="MPPLYLADEAATQDLGRALARALRPGDAVCLTGPLGAGKSTLAR
                     ALIRALTTADEEVPSPTFTLV"
BASE COUNT          207 a          458 c          375 g          196 t
ORIGIN      
        1 agccataggc gccggccgcc agaacagcca gaaccagaaa ggcgatcagc ccgcgcatca
       61 gcttcttcaa ggtccaaact cccgccgtac cctctgaccc taagctcaac cctcgcgcgc
      121 cggacgtcaa ttttcctgac atcgcccgcg acggcgccta agctctcacc atggtcaacg
      181 ttccagaggc gcgaccggcg ccccggcccc cgcacatcgt cgacacgctc atcgccgagc
      241 gtgcgccccg gctgacagcc tcgcccctgt ggccgctcct gcgccggccg ctctatgccg
      301 tgcttggcta tgacaaggcc cggcgcatgg ccgacacgat cgccgggatc ggcggtcttg
      361 cagcgctgga gcacgtctcg gccctgctgg atctgaaggt cgatgtgcgg ggtctggaaa
      421 acgtcccgac gcgcggtcgg ctggtcgtgg tcggtaacca ccccaccggc atcgccgacg
      481 gcgtcgcggt tcatgacgcc ctgaagcagg tccgcccgga caccgtgttc tatgccaacg
      541 ccgacgctca ccgcgtctgc ccaaggtttg acgaggtgct gatcccggtc gaatgggttg
      601 aggccaagcg aacgcgcgac cggatgcgcc tgaccctgca gatgacccgc caggcgatgg
      661 aggccgagcg cgccctgatg atcttcccgg cgggacgcct ggcggtgaag gatgaggagg
      721 gcctgttgtc cgatccgccc tgggccgcat cggcggtgtc gatcgctcga aaatatgaag
      781 cgccaatcat ccccatccac gtcgccggtc cgtggtcgac cctgttccat ctgttcgaca
      841 ggcgacatnn nnnnnnnnag ctgcgcgaca tcaccctttt ccatgagctg ctcaacaaga
      901 ccgggcgacg attcgcgctg atcgtcggcc gggccatcga tcccgaaagc cttgccgcgg
      961 atacggggga agccacgacc accctgaaac gctatgtcga acggaccctg ccaaagcatc
     1021 ccgaaggcgc cctgacttga gctctctccg cctgccgccc ctctacctcg cagacgaggc
     1081 cgccacccag gatcttggcc gcgccctggc ccgcgcgcta cggccgggcg acgccgtctg
     1141 cctgacgggt ccgctggggg ccggcaagtc gaccctggcc cgggctctga tccgcgccct
     1201 gaccacggcc gatgaagagg tccccagccc gaccttcacc ctcgtg
//
LOCUS       DAOMEO010001108         1246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11070_length_1246_cov_2.392107, whole genome shotgun sequence.
ACCESSION   DAOMEO010001108 DAOMEO010000000
VERSION     DAOMEO010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_1246_cov_2.392107"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1246
                     /locus_tag="PLH31_18850"
     CDS             <1..>1246
                     /locus_tag="PLH31_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HQR91288.1"
                     /translation="MQSNGGLTDARAFRGKDAILSGPAGGVVGMARTAAQAGFDRVIG
                     FDMGGTSTDVCHYAGAYERAFETVVAGVRMRAPMMNIHTVAAGGGSICSFDGARLRVG
                     PASAGAVPGPAAYRRGGPLTVTDCNVRLGKLRPEFFPAVFGPNADQPLDALAVTRGFD
                     ALAADILTATGEAMSPEAIAEGFLTIAVENMAKAVRQISIQRGYDVTRYVLACFGGAG
                     GQHACLVADALGMTKVMIHPFAGVLSAYGMGLADLRLIREATVEQTLEEATDLAEQAR
                     VLSVEAEALLRAQAVPLVSIETLATLRVKYAGTDTPLVVPLANEAGVRAAFEEMHQRR
                     FGFTSPTTALVVETLSVEAIGHSDAGGAPDFGSGAVGDPLPLTTVDVRMAGKVRATPV
                     FDRETLAVGAEIKGPAIIREATG"
BASE COUNT          176 a          413 c          450 g          207 t
ORIGIN      
        1 catgcagtcg aacggcggtt tgaccgacgc gcgggccttc cggggcaagg acgccattct
       61 gtcgggtccc gccggcggcg tggtcggcat ggcgcggaca gcggcgcaag ccggcttcga
      121 ccgcgtgatc ggcttcgaca tgggcggcac ctcgaccgac gtctgccact atgccggcgc
      181 gtacgaacgg gcctttgaga ccgtggtggc cggcgtgcgg atgcgcgcgc cgatgatgaa
      241 catccacacc gtggcggccg gcggcggctc gatctgcagc ttcgacggcg cgcgcctgcg
      301 ggtgggaccg gcgtcggccg gcgctgtgcc aggtcccgcc gcctatcgcc ggggcgggcc
      361 gctgacggtc accgactgca acgtcaggct gggtaagctg cggccggaat tcttcccggc
      421 ggtgttcggc cccaatgccg atcagccgct ggatgcgctg gcggtgacgc gcgggttcga
      481 cgcgctcgcc gccgacatcc tgaccgccac cggcgaggcc atgagccccg aggccatcgc
      541 cgagggcttc ctgaccatcg ccgtcgagaa catggccaag gccgttcggc agatctcgat
      601 ccagcgcggc tatgacgtca cccgctatgt gctggcctgc ttcggcgggg cgggcgggca
      661 gcacgcctgc ctggtggctg atgctctggg catgacgaag gtgatgatcc atccgttcgc
      721 cggggtattg tccgcctacg gaatgggcct ggccgatctg cgtcttatcc gcgaggcgac
      781 ggtcgagcag acgctggagg aggcgaccga tctggctgag caggcaaggg tgctctctgt
      841 cgaggccgag gccttgctgc gcgcccaggc cgtgccgttg gtctcgatcg agaccctggc
      901 gacccttcgc gtcaaatatg ccgggaccga cacgccgctg gtcgtgccgc tggcgaacga
      961 ggccggcgtc cgcgcggctt ttgaagagat gcaccagagg cggttcggct tcacctcacc
     1021 aacgacggcc ctggtggtcg agacgttgtc ggtggaggct atcggccatt cggatgccgg
     1081 aggcgcacca gatttcggct ctggcgccgt tggtgatccc ttgcccctga cgaccgttga
     1141 cgtccgcatg gccggaaagg tccgcgccac gccggtgttc gaccgcgaga ccctggccgt
     1201 tggcgctgaa atcaagggcc ccgccataat ccgcgaggcg actggc
//
LOCUS       DAOMEO010001109         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11098_length_1244_cov_2.450799, whole genome shotgun sequence.
ACCESSION   DAOMEO010001109 DAOMEO010000000
VERSION     DAOMEO010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_1244_cov_2.450799"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLH31_18855"
     CDS             complement(<1..484)
                     /locus_tag="PLH31_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR91289.1"
                     /translation="MSKLPDLEGLAIFAKVVELRSFAGAAEELGLSKATVSKAVTRLE
                     TRLGARLFNRTSRRLALTDAGQSLVEPATRVLVQGEEAENAAMAQSAAPRGLVRLAVP
                     MSFGLSDVAPILPAFLAAYPEVSVDLHLSDATVDLVGMGFDAALRIAALPDSSLVARR
                     L"
     gene            621..>1244
                     /locus_tag="PLH31_18860"
     CDS             621..>1244
                     /locus_tag="PLH31_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQR91290.1"
                     /translation="MIDRRPFDRLGGADHGWLKAKHHFSFASYYDPKNMNWGALRVWN
                     DDEIAPNTGFPPHPHSDMEIITYVRSGAITHQDNLGNTGRTEAGDVQVMSAGTGIRHS
                     EYNLEPEATRIFQIWIEPNRTGGAPSWGAKPFPKGDRSGKFVTLASGFDNDGDVLPIR
                     TDARVLGATLNAGESTTYDLGKSRHAYLVPAVGSVTVNGVQLNARDGA"
BASE COUNT          241 a          426 c          394 g          183 t
ORIGIN      
        1 gcaggcggcg ggccaccaga gatgaatccg gcagggcggc gatgcgcagg gcggcgtcaa
       61 agcccatgcc caccagatcc accgtggcgt cgctgagatg caggtcgacc gaaacctcgg
      121 gataggcggc caggaacgcc ggcaggatcg gcgcgacgtc ggacaggcca aacgacatcg
      181 gaaccgccag ccgcaccagg ccgcgcggcg cagccgactg ggccatggcg gcgttctcgg
      241 cctcctcgcc ctggaccagc acgcgggtgg cgggctcgac caggctctgg ccggcgtcgg
      301 tcagggccag ccgtcgtgag gtgcggttga acagccgcgc gcccagccgc gtttccagcc
      361 gggtgacagc cttggacacc gtggccttgg acaggcccag ctcctcggcg gcgcccgcga
      421 aggaccgcaa ttcgaccacc ttggcgaaga tcgccaggcc ctcaagatca ggaagtttgc
      481 tcattttgga aacaatggat ttcaatagtt tctatttagg ttccaatcct gatcgctatc
      541 ttcacctcaa gcaagcggcg tcgatgaccg gcgcgctttc aagaacgagc ccggcgaccg
      601 ggctccagga ggttaaggcc atgatcgatc gcagaccgtt cgacaggctt ggcggcgccg
      661 accacggttg gctgaaggcc aagcatcact tctcgttcgc cagctactat gaccccaaga
      721 acatgaactg gggcgccctg cgggtttgga acgacgacga aatcgccccc aacaccggct
      781 tcccgcccca cccgcactcg gacatggaaa tcatcaccta tgtccgcagc ggcgcgatca
      841 cccaccagga caacctgggc aatacgggcc gcaccgaggc gggcgacgtt caggtgatga
      901 gcgccggcac cggcatccgt cactccgaat ataatctgga gccggaagcc acgcggatct
      961 tccagatctg gatcgagccc aaccgcaccg gcggtgcgcc gtcctggggc gccaagcctt
     1021 tccccaaggg cgaccgttcg ggcaagttcg tgaccctggc cagcggcttc gacaatgacg
     1081 gcgacgtcct gccgatccgc accgacgccc gcgttctggg cgccaccctg aacgccggcg
     1141 aaagcacgac ctatgacctg ggcaagagcc gccacgccta tctggtgccg gcggtaggtt
     1201 cggtcaccgt caatggcgtc cagctgaacg cccgcgacgg cgcg
//
LOCUS       DAOMEO010001110         1244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11099_length_1244_cov_2.417157, whole genome shotgun sequence.
ACCESSION   DAOMEO010001110 DAOMEO010000000
VERSION     DAOMEO010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11099_length_1244_cov_2.417157"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..686
                     /locus_tag="PLH31_18865"
     CDS             183..686
                     /locus_tag="PLH31_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR91291.1"
                     /translation="MRFDTLKILLVDDNQHMRLLLTEMLSAIDVRHIFEAVDGAEALH
                     MMRRVPIDIVITDLKMGLLGGIDFVNLLRRSPDSPNPFAPVLMVTGHSTLRRVQEARD
                     AGVNEFLTKPVTGRSLIHRLTLLIDQPRPFIRCDGYFGPDRRRRVTSDYKGPWRRDED
                     AVSIDFG"
     gene            756..1004
                     /locus_tag="PLH31_18870"
     CDS             756..1004
                     /locus_tag="PLH31_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91292.1"
                     /translation="MTVQRLISAVALAALGLSLQGCLAAAAVGTVAGTAIGVTGAVVG
                     GAAKVGGKAVVATGKAVIPGESAKDREKREYEEWKRSN"
     gene            complement(1008..>1244)
                     /locus_tag="PLH31_18875"
     CDS             complement(1008..>1244)
                     /locus_tag="PLH31_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQR91293.1"
                     /translation="GPMQDAGWVADLDGLDSALKAVATELDHGLLNDRPGLESPTLER
                     LCLYFAERLTPAFPGLSRVVISRPTIGESCALSL"
BASE COUNT          226 a          414 c          386 g          218 t
ORIGIN      
        1 tcgaccgctt ttgagacctt tggtcagtga cctttgctta aggtggtctg acaactgaaa
       61 acattcaggt ttttgggttg accgcataga ttgccaagct gtaatgtttc atccagcgcg
      121 aacatcacgg cctcttctct cgcaatagtt gcgtcagcgc gcgtcaaggc tctagaacac
      181 gcgtgcgttt cgacactctg aaaatcctgc tcgtcgacga caatcagcac atgcggctgc
      241 tgttgaccga aatgctcagc gccatcgacg tgcggcacat cttcgaggcc gtggacggcg
      301 ccgaggcgct gcacatgatg cggcgggtgc cgatcgatat cgtcatcacc gacctgaaga
      361 tgggcctgct gggcgggatc gacttcgtca acctgctgcg ccgctcgccc gacagcccca
      421 atcctttcgc tccggtgctg atggtcacgg gtcattcgac attacggcgc gtgcaggagg
      481 cgcgcgacgc cggggtcaac gagttcctga ccaagccggt gaccggccgc agcctaatcc
      541 atcgcctgac cctgctgatc gatcagcccc gcccgttcat ccgctgcgac ggctatttcg
      601 gccccgaccg gcggcgacgc gtcacctcgg actacaaggg gccctggcgg cgggacgaag
      661 acgcggtctc gatcgacttc ggctgaccgg tcgctcatca ggtcttaaga cctgttagcc
      721 atcatggcgc cgctgttcac cgacaagagc gcgccatgac cgttcaacgc ctgatctccg
      781 ccgtcgcgct cgccgcgctt ggcctgtccc tgcaaggctg cctcgctgca gcggccgtgg
      841 gcaccgtagc cggcaccgcc atcggcgtca ccggcgccgt ggtcggcggc gccgccaagg
      901 tcggcggcaa ggccgtggtc gccaccggca aggccgtgat ccccggcgaa agcgccaagg
      961 atcgcgagaa gcgagaatac gaagagtgga agcgctcgaa ctgaggccta caggctgagc
     1021 gcgcagctct cgccgatcgt cgggcgcgag atcaccacgc gggacagacc tggaaaggcc
     1081 ggcgtcaggc gctcggcgaa atagagacac aaccgctcca gggtcgggct ttccagacca
     1141 gggcgatcat tgagcaggcc atgatccagc tcggtcgcca cggccttcag cgcggagtcc
     1201 agaccgtcga ggtcggccac ccagccggcg tcctgcatcg gccc
//
LOCUS       DAOMEO010001111         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11122_length_1242_cov_2.510531, whole genome shotgun sequence.
ACCESSION   DAOMEO010001111 DAOMEO010000000
VERSION     DAOMEO010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_1242_cov_2.510531"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..682
                     /locus_tag="PLH31_18880"
                     /pseudo
     CDS             170..682
                     /locus_tag="PLH31_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113068.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
     assembly_gap    683..692
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <693..>1242
                     /locus_tag="PLH31_18885"
     CDS             <693..>1242
                     /locus_tag="PLH31_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQR91294.1"
                     /translation="ADRLLSDDQLSQTLKICGGLVQPMPSALSEPQASEARVKRLYGV
                     DTLDGFGGLSPAEIGALGLIAAHLEMTQAGRLPALRAPRRAADADVMAIDPATRGSLE
                     IDRTQTGDRNGSLLAAIDRTVTAGGARLLASRLARPLLDPAAIDQRLDAVEWFVEHRA
                     LRERVREVLKGSGDMARALSRLA"
BASE COUNT          183 a          438 c          417 g          194 t
ORIGIN      
        1 ctgcctcttc aagcgcagtt gtcatggaat ccatgacagc tgaactggtc gtcaacggcc
       61 ccctcgctca tgccgcccct ctcaatggcc gaacaaacct tcagcacaaa acaggaacat
      121 tctacatggc gcttctgcga agcagcgtca aacgttaagt catggcgtca tgaacgcccc
      181 cgcctccgct tccgtccaga cctatgacgc caccggcgcg acgccggtga tgcagcagtt
      241 cttcgagatg aaggcgcggc agccggacgc cctgatcttc tttcggatgg gcgatttcta
      301 tgagctgttc ttcgacgacg cgatccgggc ggcggcggcg ctgggcatct cccagacgtt
      361 tcggggtacg cacaatagcc agccgatccc gatggccggg gtgccgcagc atgcggccga
      421 ggcctacctc tccaagctga tccgcctggg cttcaaggtc gccgtctgcg agcagatgga
      481 ggaccccgcc gaggcgaaaa agcgcggctc caagtcgatc gtccgccgcg acatcgtgcg
      541 ggtggtcacc cccggcaccc tgaccgagga cggcctgctg gacgctcgcg gcgccaatcg
      601 cctggcggcc gtcgctgtgc gcgccgggca ggccgctgtg gcctcggtcg agctgtcgac
      661 cggcgaggtc gaggtgttct cgnnnnnnnn nngccgaccg gctgctgtcc gacgaccagc
      721 tgtcccagac cctgaagatc tgcggcgggc tggtgcagcc catgccgtcg gccctgtcgg
      781 agccgcaggc gtccgaggcg cgggtcaagc gcctctatgg ggtcgacacc ctggacggtt
      841 tcggcggcct gtcgccggcc gagatcgggg cgctgggcct gatcgccgcc catctggaaa
      901 tgacccaggc cggacgcctg ccggccctgc gcgcgccgcg gcgggcggcc gacgccgacg
      961 tcatggccat cgacccggcc acccggggca gtctggagat cgaccgcacg cagaccggcg
     1021 accgcaatgg ctcgctgctg gccgccatcg accgcaccgt caccgccggc ggcgcgcgcc
     1081 tgttggcctc gcggttggcc aggccgctgc tggaccccgc cgccatcgac cagcgcctgg
     1141 acgcggtgga atggttcgtc gagcaccggg ccctgcgcga gcgggtccgt gaggtgctga
     1201 agggttcggg tgacatggcc cgcgccctgt cgcgcctggc gc
//
LOCUS       DAOMEO010001112         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11123_length_1242_cov_2.294019, whole genome shotgun sequence.
ACCESSION   DAOMEO010001112 DAOMEO010000000
VERSION     DAOMEO010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11123_length_1242_cov_2.294019"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /gene="ssb"
                     /locus_tag="PLH31_18890"
     CDS             complement(<1..174)
                     /gene="ssb"
                     /locus_tag="PLH31_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR91295.1"
                     /translation="MAGSVNKVILVGNLGADPEIRSLGSGDRVANLRIATSESWRDRT
                     SGERKEKTEWHRVV"
     assembly_gap    300..309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(311..706)
                     /locus_tag="PLH31_18895"
     CDS             complement(311..706)
                     /locus_tag="PLH31_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91296.1"
                     /translation="MRRPILIAASVAAAGLALAGCEKQIKAPFEKGVCFHVVTNKDKT
                     LRFNPVERNVPSMEQCAATLEGMRIRFVRMGSANRTLTGSYQGSFIFVEKEGIYLGQT
                     YDGARFMSLVRTGDGRLAVPGAIREVPAP"
     gene            complement(720..>1242)
                     /locus_tag="PLH31_18900"
     CDS             complement(720..>1242)
                     /locus_tag="PLH31_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91297.1"
                     /translation="SDDTKLDPARPLRCFVGGQFVGETTLSECAKKNGVATGALDVGV
                     DETGALAAADQAGTVLTPLPPPTVATPPPSTIAPGPAPSPTATASTTPLAACWRYSGA
                     EWRRLPGDITLSACVQTLFAGRCERPGGATYGRWGEETLRLVPGRVEASFDNRSFHTV
                     TDQGPNCSIPAAG"
BASE COUNT          224 a          391 c          400 g          217 t
ORIGIN      
        1 cacgacccgg tgccattcgg ttttttcctt gcgctcgccc gatgtgcgat cacgccagct
       61 ttccgacgtg gcaatacgca gattggcgac gcggtcaccg gaacccaggc tgcggatttc
      121 gggatcggcc ccgaggttac ccaccagaat gaccttgttg acgctgcccg ccatcacttt
      181 gaccttgttc acgcccggcg acccttgcgc cggaatctga tcaagacgtt aacggagggc
      241 cggggtcaac tcaatttgtt ctcaatacgt tcctgtggac gacgggcggt tgagcctaan
      301 nnnnnnnnng ttagggcgcc gggacctcgc ggatcgcgcc cggaaccgcc agacggccgt
      361 ctccagtcct caccagagac atgaagcgag cgccatcata ggtctggccc agatagatcc
      421 cttccttctc cacgaagatg aagctgccct gatacgaccc cgtaagcgtc cgattggcgc
      481 tgcccatgcg aacaaagcgg atgcgcatgc cttccagtgt cgccgcgcac tgctccatgg
      541 atggaacgtt gcgctccacc ggattgaacc gaagcgtctt gtccttgttg gtcacgacgt
      601 ggaaacagac acccttttcg aagggggcct tgatctgctt ttcgcaaccc gccagcgcaa
      661 ggccggctgc ggcgacggag gcggcgatca ggatcggacg gcgcatggcg atactcgtct
      721 tagccggcgg ccgggatcga gcagttcggt ccctggtcgg tcacggtatg gaaggaacga
      781 ttgtcgaagg aggcttcaac acggccagga accaaccgaa gggtttcctc gccccaccgg
      841 ccgtatgtgg cgccgccggg tcgctcgcag cgaccggcga acaatgtctg aacgcaggcg
      901 ctcagcgtga tgtccccggg gagacgacgc cattcggccc cggaatagcg ccaacacgcc
      961 gccagcggcg tcgtggacgc ggtcgcagtc ggcgacggag cgggtccagg tgcgatggtc
     1021 gaaggcggcg gggttgcgac ggtcggcggc ggcagcggcg tcagaaccgt gccagcttga
     1081 tcggccgcgg ccagggcgcc ggtttcgtca acgccaacat ccagcgcgcc cgtcgcgacg
     1141 ccgttcttct tggcgcattc ggaaagggtg gtctcgccga cgaactgccc gccgacgaaa
     1201 caacggagcg ggcgcgcagg gtcaagtttg gtgtcgtcgg aa
//
LOCUS       DAOMEO010001113         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11124_length_1242_cov_2.273799, whole genome shotgun sequence.
ACCESSION   DAOMEO010001113 DAOMEO010000000
VERSION     DAOMEO010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11124_length_1242_cov_2.273799"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLH31_18905"
     CDS             complement(<1..234)
                     /locus_tag="PLH31_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91298.1"
                     /translation="MIRALLCSSALSTVLISGAVMAEEQRAPETDVAGRTGATALAEL
                     VVTATRTAQPIEKVGASITVLTSEAIKASQATTA"
     regulatory      complement(373..561)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            720..>1242
                     /locus_tag="PLH31_18910"
     CDS             720..>1242
                     /locus_tag="PLH31_18910"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQR91299.1"
                     /translation="MRILALGLTVSMSILAACATPPGAGGSVPLAAFFDCARDRGVLI
                     SAHRAVLDDSTPENSLAGIRATGAAIRPALLEIDVTRTRDGALVLMHDDTLNRTTNGR
                     GRVADHTLAQLRATRLIDGAGKVTDAPPPTLREALDAAGKVGSIVTLDFKSSGADGET
                     LTRQVIAETRAARA"
BASE COUNT          207 a          423 c          403 g          209 t
ORIGIN      
        1 ggctgtggtg gcctgactgg ccttgatggc ctcggacgtc agaacggtga tggacgcgcc
       61 gaccttctcg atcggctgag ccgtacgcgt ggcggtgacc accagttcgg ccagggcggt
      121 ggcgccggtc cggccggcga cgtcagtttc tggcgccctt tgctcttcgg ccatgacggc
      181 cccggagatc aagacggtgg agagtgcgct ggagcacaga agcgctcgga tcatggtctt
      241 tccctttgaa tgacccgcgc acgtcctcaa gccccaaacg caaggatgcg cccacggcga
      301 gaggccgcga cgcggcctga ctgacatgtc gtttcgcggg aaatcccgtt cgttcggcgc
      361 accccggccg gacgatgaac gactgcgccg ggcaggtctc ctggctcacg ggtctatgct
      421 tggcgcgtcg ccttcccagg accggggtcc cagtggcatg tgacgcgtaa gctcgccgct
      481 tacagttgcg ggggcagccg gggttttgaa ccccgttccc tattcatcct cttgcgagga
      541 acctgacgcg aaatcagccc tatagggtgg gccgcatacc gtcaatgtgg agcgacccga
      601 cggcggaaaa cgacacgcca aacgccaata gccctggaat ctcgccttcc tggcccggca
      661 cgccgtcggt ctgctagaca gcccaagtcc gatcgactct cgaatctgga gtgaactgca
      721 tgcgcatcct ggctcttggc cttaccgttt cgatgtcgat cctggctgcc tgcgcgacac
      781 cacccggcgc cgggggcagc gtgcccctcg cggccttttt cgactgcgct cgggacaggg
      841 gcgtgctgat ctcggctcac cgcgcggtgc tggacgacag cacgccggaa aactccctcg
      901 caggcattcg cgccacaggc gcggccatcc gccctgccct ccttgagatc gatgtcacca
      961 gaaccaggga cggcgcattg gtcctgatgc atgacgacac gctcaatcgc accaccaacg
     1021 gccgaggccg cgtggccgat cacaccctgg cgcagttgcg ggcgacgcgc ctgatcgacg
     1081 gcgccggcaa ggttacggat gccccgcccc ccaccctgcg cgaggcgctg gacgcggcgg
     1141 gcaaggtggg atcgatcgtg acgctggact tcaagagttc gggcgcagat ggcgagacgc
     1201 tgacccgaca ggtcatcgcc gaaacccgcg ccgcacgcgc cg
//
LOCUS       DAOMEO010001114         1239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11164_length_1239_cov_1.845439, whole genome shotgun sequence.
ACCESSION   DAOMEO010001114 DAOMEO010000000
VERSION     DAOMEO010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11164_length_1239_cov_1.845439"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="ftsQ"
                     /locus_tag="PLH31_18915"
     CDS             <1..307
                     /gene="ftsQ"
                     /locus_tag="PLH31_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HQR91300.1"
                     /translation="GADQAAGAILPAVAARPRLRDRLEALVRVDDRRWDLRLKDGSLI
                     QLPAIDEESALIQLDQLDQRQRILDLGFARIDLRDPEMVAVRPRDAALPGQTGAGGA"
     gene            335..>1239
                     /gene="ftsA"
                     /locus_tag="PLH31_18920"
     CDS             335..>1239
                     /gene="ftsA"
                     /locus_tag="PLH31_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQR91301.1"
                     /translation="MSRLEDRKQARDGLKATLVRQPAVAAVDLGASKVTCFIMKADGI
                     HRENRTLTTAGVGYVQSRGVRGGAIVNLDEAAQAIAQAVERAETVAGVNVQGVSICTA
                     GGQLASNRVHTQVSLGARPIADGDLSRAIASALAQVKISGRKPIHLLPIAWSVDGQRG
                     IRDPRAMFGRALGLELLVVSINENVFHTLAHCVERAHLSFEGVVAAPFASALAALEED
                     EMDLGAVCIDMGGGSTSVAVFNNGALCHVDSLAVGGGHVTQDIARGLQTSVAGAERIK
                     TLHGSAIASANEDREMIEAPPRGDDP"
BASE COUNT          190 a          426 c          423 g          200 t
ORIGIN      
        1 gggcgccgac caggccgccg gcgcgatcct gccggccgtc gccgcccgtc cacgcctacg
       61 cgaccgtctg gaagcgctgg tgcgcgtcga tgatcgccgc tgggatctgc gcctgaagga
      121 cggctcgctg atccagcttc cggcgatcga cgaagaatct gccctgatcc agctggatca
      181 gttggaccag agacagagaa tcctcgatct ggggtttgcg cgcatcgacc tgcgtgatcc
      241 cgagatggtg gcagtgagac ctcgcgacgc ggctctgcct gggcagaccg gggccggcgg
      301 ggcgtagcgt gatggtgttg gggtgacgat agcgatgtcg cgactggaag atcggaaaca
      361 ggcccgtgac ggcttgaagg cgacgctcgt tcgtcagcct gcggtcgcgg ctgtcgatct
      421 gggcgcgtcc aaggtcacct gcttcatcat gaaggccgac ggcattcatc gcgaaaaccg
      481 caccctgacc acggccggcg tcggctatgt gcagtcgcgc ggcgtacgcg gcggggccat
      541 cgtcaatctg gacgaggcgg cgcaagccat cgcccaggcc gtcgagcgcg ccgagaccgt
      601 ggccggcgtc aacgtccagg gcgtcagcat ctgtacggcc ggcggtcagc tggccagcaa
      661 ccgcgttcac acccaggttt cgctgggcgc ccggcccatc gccgacggcg acctttcgcg
      721 cgccatcgcc tcggccctgg cccaggtcaa gatttccggt cgtaagccaa tccacctgct
      781 gcccatcgcc tggtcggtcg acggccagcg cggcattcgc gatccgcgcg ccatgtttgg
      841 tcgcgccctg ggcctggaac tgctggtcgt ctcgatcaac gagaacgtct tccacactct
      901 ggcccactgt gtcgagcgcg cccacctgtc cttcgagggc gtggtcgccg cgcccttcgc
      961 ctcggccctg gcggccctgg aagaggacga gatggaccta ggcgccgtct gcatcgacat
     1021 gggcggcggc tcgacctcgg tggcggtgtt taacaatggc gccctgtgcc acgtcgacag
     1081 cctggctgtc ggcggcgggc acgtgacgca ggatatcgcg cgcggcctgc agacctcggt
     1141 cgccggcgcc gagcgcatca agaccctgca cggcagcgcc atcgcctcgg ccaatgaaga
     1201 ccgcgagatg atcgaagccc cgccgcgcgg cgacgatcc
//
LOCUS       DAOMEO010001115         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11172_length_1238_cov_2.769231, whole genome shotgun sequence.
ACCESSION   DAOMEO010001115 DAOMEO010000000
VERSION     DAOMEO010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11172_length_1238_cov_2.769231"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLH31_18925"
     CDS             complement(<1..457)
                     /locus_tag="PLH31_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91302.1"
                     /translation="MNLDFSPEDVAFRDEVRAFIAENYPAGLREKQEEGEEMAKEDFL
                     SWHRVLAKKGWVAPAWPEKFGGPGWTSVQRYIWSEETARADCVPILPFGINMVGPVIY
                     TFATPEQKERFLPQTLSGDIWWCQGYSEPGAGSDLASLKTKAERFTGDDG"
     gene            complement(591..>1238)
                     /locus_tag="PLH31_18930"
     CDS             complement(591..>1238)
                     /locus_tag="PLH31_18930"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HQR91303.1"
                     /translation="SGGATVCRLAGVQGAGLEAFDLAVDRPTPNAVDKAAMSEKECAA
                     TMAFGMEALAKQPDLLVPGVIAAETDMIASSIALALFGGEASDWTDAPDLVVAAVERT
                     REEGGLDDPLQVLRQLGGRETAAVAGAILAARVQKIPVLLDGYAACAAAAVLYQIDAA
                     LLDHCLAAHVGTAKGHGRLLAAIGKRPLLTLDIGEEEGVGGASALALVKLACEVR"
BASE COUNT          210 a          417 c          402 g          209 t
ORIGIN      
        1 tgccgtcgtc gccggtgaag cgctcggcct tggtcttcag gctggccagg tcggagcccg
       61 cgcccggctc ggaatagccc tggcaccacc agatgtcgcc cgacagggtc tgcggcagga
      121 accgctcctt ctgttcgggc gtggcgaagg tgtagatcac cgggccgacc atgttgatgc
      181 caaagggcag gatcggcacg cagtcggcgc gggcggtctc ttccgaccag atgtagcgtt
      241 gaaccgacgt ccagcccgga ccgccgaact tttcaggcca ggccggcgcg acccagccct
      301 ttttggcgag gacccgatgc catgacagga agtcctcctt ggccatttcc tcaccctctt
      361 cctgcttttc acgcaggcca gcagggtaat tctcggcgat gaaggcgcgg acttcgtcgc
      421 ggaaagcgac gtcttctggc gaaaagtcga gattcatggc gggctcccgg tttcggcctc
      481 tttgatgagg ggccgattga tctgggcgct acgatagatg tcgaacggac gtttggaaag
      541 atgacgttag cgtcaacgga agcgaatatg atcctcgttc agttagcgcc ctagcgaacc
      601 tcgcaagcca gcttcaccaa ggccagagcg ctggccccgc ccacgccctc ctcctcgccg
      661 atatcaagcg tcagcagcgg ccgtttgccg atggccgcca gcaatcggcc gtggcccttg
      721 gccgttccca catgggcggc gagacagtgg tccagcaggg ccgcatcgat ctgataaagg
      781 acggcggcgg cggcgcaggc ggcgtagccg tccagcagaa ccgggatctt ctggacccgc
      841 gccgcgagga tggcgccggc cacggcggct gtctcgcgtc cgcccagttg gcgcagcacc
      901 tgaaggggat catccagccc cccctcctct cgggtccgct caacggcggc gaccaccaga
      961 tccggcgcat cggtccagtc gctggcctcg ccaccgaaaa gggcgagcgc gatcgacgag
     1021 gcgatcatgt cggtctcggc ggcgataacg cccggaacca gcaggtcggg ctgcttggcc
     1081 agggcctcca tgccaaaggc catggtggcg gcgcattcct tttcgctcat cgcggccttg
     1141 tcgacagcgt tgggcgttgg ccgatccacg gccagatcaa aggcctccag gcccgcgccc
     1201 tgaacgccgg ccaggcggca caccgtcgcg ccgccgga
//
LOCUS       DAOMEO010001116         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11173_length_1238_cov_2.763314, whole genome shotgun sequence.
ACCESSION   DAOMEO010001116 DAOMEO010000000
VERSION     DAOMEO010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11173_length_1238_cov_2.763314"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1238)
                     /locus_tag="PLH31_18935"
     CDS             complement(<1..>1238)
                     /locus_tag="PLH31_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR91304.1"
                     /translation="GVTLTLAAIGFDLALPYAAGHLVDAVVEKPRGDPAAWSALWVFV
                     GVYLAFAVVRNISHRFWIPLAARNMEEMTNEGFARVQAFSSDWHADSFAGATVRKLTR
                     AMWGYDSVTDAVTIWLGPGIVVLVGLSVMMLFRQPLAGVISLVVIAAYLAANIIVIER
                     YVRPHNLRSNALDSKIGGALADAVTSNPTVKSFGAEDRETARIAQVTAQWRQAVMITW
                     GRFTDVWLGQNLLLVLLQAGLTGSMVWGWSRGTASPGDVAFAITAFMLMSGYLRNLGE
                     NIRMLQKGLDDAEDVAAWDRLAPQIADAPGAPAFRVGPGAIAFEDVTFRYKAAGAPLY
                     DRFSLTIAPGERVALVGATGSGKSTFVKLIQRLHDLQGGRIVIDGQDVSVVTQGSLRR
                     AIAVVPQDPALFHRTLSENIA"
BASE COUNT          211 a          433 c          424 g          170 t
ORIGIN      
        1 gcgatgtttt ccgagagcgt gcggtggaac agggccgggt cctggggcac caccgcgatg
       61 gcgcggcgca gggagccctg cgtcaccacc gagacgtcct ggccatcgat gacgatccgg
      121 ccgccctgca ggtcgtgcag gcgctggatc agcttgacga aggtcgactt gcccgagcct
      181 gtcgccccca ccagggccac gcgctcgccc ggcgcgatgg tcagcgagaa gcggtcatac
      241 agcggcgcgc cggcggcctt gtagcggaag gtcacgtcct cgaaggcgat ggcgccgggg
      301 ccgaccctga aggccggcgc gccgggcgcg tcggcgatct gcggcgccag gcgatcccag
      361 gcggccacgt cctcggcgtc atccagaccc ttctgcagca tacggatgtt ttcacccaga
      421 ttgcgcagat agccgctcat cagcatgaag gcggtgatgg cgaaggccac gtcgccgggg
      481 ctggccgtgc cccgcgacca gccccagacc atcgagccgg tgaggccggc ctgcagcagg
      541 accagcagca ggttctgacc cagccacaca tcggtgaacc ggccccaggt gatcatcacc
      601 gcctggcgcc actgcgccgt gacctgggcg atgcgggcgg tctcccggtc ttcggccccg
      661 aagctcttga cggtcgggtt ggaggtcacc gcatccgcca gggccccgcc gatcttcgag
      721 tccagggcgt ttgagcgcag gttgtggggc cgcacatagc gctcgatcac gatgatgttg
      781 gcggccagat aggcggcgat caccaccagc gagatcaccc ccgccagggg ctggcgaaac
      841 agcatcatca ccgacaggcc gaccagcacc acgatgccgg ggcccagcca gatggtcacc
      901 gcgtcggtga cgctgtcata gccccacatg gcgcgcgtca gcttgcgcac cgtggctccg
      961 gcgaagctgt cggcgtgcca gtccgacgag aaggcctgca cccgggcgaa gccctcgttg
     1021 gtcatctctt ccatgttgcg ggcggccagc gggatccaga accggtgcga gatattccgc
     1081 accaccgcaa aggccagata gacgccgacg aacacccaca gggccgacca ggcggccggg
     1141 tcgccgcgcg gcttttcgac caccgcgtcc accagatggc cggccgcata gggcagggcc
     1201 aggtcgaaac cgatggccgc cagggtcagg gtcacgcc
//
LOCUS       DAOMEO010001117         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11185_length_1237_cov_3.056684, whole genome shotgun sequence.
ACCESSION   DAOMEO010001117 DAOMEO010000000
VERSION     DAOMEO010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11185_length_1237_cov_3.056684"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLH31_18940"
     CDS             complement(<1..887)
                     /locus_tag="PLH31_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR91305.1"
                     /translation="MTSPTSSKSSQQHIMGVYNRAPLAFERGRGARLFSTDGDEYLDC
                     VAGIATDGLGHAHPVLVEALKTQGEKLWHVSNIYRIPEQEELADKLCAHSFADVVFFT
                     NSGTEAIECALKTARKYHTANGQPERTEIFGFDGSFHGRSYAAVNASGNPSYVEGFGP
                     RLPGYSQLTFGDHDALKAAIAQPTTAAIIVEPVQGEGGARSIPEHCLVSLRSLCDEHG
                     VLLIYDEVQCGMGRTGKLFAYEWAPGGEPHIMAVAKALGGGFPVGACLATTEAAKGMT
                     VGVHGSTFGGNPLAMAVGKA"
     gene            complement(1024..>1237)
                     /locus_tag="PLH31_18945"
     CDS             complement(1024..>1237)
                     /locus_tag="PLH31_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HQR91306.1"
                     /translation="TFYLVDKLPEPFRTASHYNPFFYLIDGFRYGFIGHADGSILVGV
                     VTTAALVVALFLWCWQLFRTGYRLKT"
BASE COUNT          218 a          390 c          431 g          198 t
ORIGIN      
        1 gccttgccca cggccatggc cagcgggttg ccgccgaagg tcgagccatg gacgcccaca
       61 gtcatgccct tggccgcctc ggtggtggca aggcaggcgc cgaccgggaa gccgccgccg
      121 agcgccttgg ccaccgccat gatgtgcggc tcgccgccgg gcgcccactc gtaggcgaac
      181 agcttgccgg tccggcccat gccgcactgc acttcgtcat agatcagcag gacgccgtgc
      241 tcgtcgcaca gcgaacgcag gctgaccagg cagtgctcgg ggatcgaccg cgcgccgccc
      301 tcgccctgca cgggctcgac gatgatcgcc gccgtggtcg gctgggcgat agcggccttc
      361 agggcgtcgt gatcgccaaa ggtcagctgg ctgtagcccg gcagacgggg accaaagccc
      421 tcgacatagc tgggattgcc cgaggcgttg accgccgcat aggagcggcc gtggaacgag
      481 ccgtcgaagc cgaagatctc ggtccgctcg ggctggccat tggcggtgtg atacttgcgg
      541 gccgtcttca gcgcgcactc gatcgcctcg gtgcccgaat tggtgaagaa cacgacgtcg
      601 gcgaagctgt gggcgcacag cttgtcggcc agctcttcct gttcggggat gcgatagatg
      661 ttggagacgt gccagagctt ctcgccttgc gtcttcagcg cctcgaccag caccgggtgg
      721 gcgtggccca gaccgtcggt ggcgatgccg gccacgcagt ccagatactc atcgccgtcg
      781 gtcgagaaca ggcgcgcgcc tcggcctcgc tcaaacgcca gcggggcgcg gttgtagacg
      841 cccatgatgt gctgttgcga ggacttcgaa gacgtcgggc tggtcaagac cgcctcccaa
      901 aaaggaaaga agcccccgcg ctgttcgcac ggggaccgga aggccgcagg ttgtcgccgt
      961 ggaacgacgc tctgtcaatg aaagtacggt gaatttagcc gcttgttgct cgcgggtgac
     1021 ccttcaggtc ttgaggcggt agccggtgcg gaacagctgc cagcaccaca ggaacagcgc
     1081 gaccaccaga gcggccgtgg ttacgacgcc gaccaggatc gagccgtcgg catggccgat
     1141 gaagccgtag cggaagccgt cgatcagata gaagaacgga ttgtagtggc tggcggtgcg
     1201 gaacggctcg ggcagcttgt cgaccagata gaaggtg
//
LOCUS       DAOMEO010001118         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11187_length_1237_cov_2.780880, whole genome shotgun sequence.
ACCESSION   DAOMEO010001118 DAOMEO010000000
VERSION     DAOMEO010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11187_length_1237_cov_2.780880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..921)
                     /locus_tag="PLH31_18950"
     CDS             complement(154..921)
                     /locus_tag="PLH31_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR91307.1"
                     /translation="MTDHTLAGRTVLVTGASSGIGAHLALAAAQAGASVALAARRTDR
                     LADLVSQIQAAGGKAQAVAMDVEDEASVIAAYDAIEAGLGGVDSVIANAGISVQGSAL
                     DIAIDDFDKVLAVNTRGMFLTAREGARRMIKAGCPETGRGRMLLVASIGAHKVLPGLT
                     AYCTSKAGTAMMGKSLAREWARKGINVNVLCPGYIRTELNSDWFDQEGGQKLVASFPR
                     KRLMAESDLAPMALHLLSDASATITGGVFTVDDGQSL"
BASE COUNT          175 a          436 c          442 g          184 t
ORIGIN      
        1 ggagctgggg cccgaatgga cgccccggcc tttcgagctg aaggccatcc agctggccgg
       61 gcgcatggcc gaccgcatcg ccgtgcccgg cgcgccgccg gctctggctt gccagcggat
      121 ggggctgccg gcggactggc tgtatcgccg cccttagagc gactgaccgt catccaccgt
      181 gaacaccccg ccggtgatcg tggccgaggc gtccgacagc aggtgcaggg ccatgggcgc
      241 caggtcgctt tccgccatca ggcgcttgcg cgggaagctg gcgaccagct tctggccgcc
      301 ctcctggtcg aaccagtcgc tgttcagctc ggtgcggata tagccggggc acaggacgtt
      361 gacgttgatg cccttgcggg cccattcgcg ggctaggctc ttgcccatca tggcggtccc
      421 ggccttggac gtgcagtagg ccgtcagccc cggcaacacc ttgtgcgccc cgatcgaggc
      481 caccagcagc atccggccgc gaccggtctc gggacagccc gccttgatca tccgccgggc
      541 gccctcgcgg gcggtcagga acatgccgcg ggtattgacc gccagcacct tgtcgaagtc
      601 gtcgatggcg atgtccaggg ccgagccctg gacgctgatc ccggcattgg cgatcacgct
      661 gtccaccccg cccagaccgg cctcgatggc gtcataggcg gcgatcaccg aggcctcgtc
      721 ctcgacgtcc atggccacgg cctgggcctt gccgccggcc gcctggatct ggctgaccag
      781 gtcggcgagc cggtcggtgc gccgcgcggc aagggctacc gaagctcccg cctgggccgc
      841 cgccagggcc agatgagcgc cgatgccgga ggaggcgccg gtcaccagca cggtgcggcc
      901 ggcaagggtg tggtcggtca tggtcagggc tctccggaac aggatccgga aggtggcggg
      961 gctggcgcgc cgtgtcgagg gcgccgttgg ggagcccttc tccccttgcg ggagaaggtg
     1021 gcgcgcagcg ccggatgagg ggttgaaaga cctgtcggaa aggcccgtgc gacccctcac
     1081 ccgacccgct gtcgcgggcc accctctccc gcaaggggga gagggaaatc tagtacggca
     1141 ggtcgtccgg cgtaggctga acgggctcct ccaccactgg cggattggcc acggcctcca
     1201 gctccgccgc cacccggcca aactcggccg ccagggt
//
LOCUS       DAOMEO010001119         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11188_length_1237_cov_2.671743, whole genome shotgun sequence.
ACCESSION   DAOMEO010001119 DAOMEO010000000
VERSION     DAOMEO010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11188_length_1237_cov_2.671743"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1237)
                     /gene="dnaE"
                     /locus_tag="PLH31_18955"
     CDS             complement(<1..>1237)
                     /gene="dnaE"
                     /locus_tag="PLH31_18955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQR91308.1"
                     /translation="DMRKLFSDLPEACDNTLDIARRCAFLVKKRDPILPSFPTGAGRS
                     EPEELTYQAREGLRARLADNVLAVDEQVYWDRLDFELGIIIKMGFPGYFLIVSDFIKW
                     AKSHGIPVGPGRGSGAGSLVAWVLTITDLDPLRFGLLFERFLNPERVSMPDFDIDFCQ
                     ERREEVIVYVQEKYGRDRVAQIITFGSLQARAVLRDVGRVMQLPLGLVDRLCKMVPNN
                     PAAPVTLAQAIELEPRLKQAKKEDGAVSACLDVALQLEGLFRNASTHAAGVVIGDRPL
                     TQLTPLYKDPRSDLPATQFNMKWVESAGLVKFDFLGLKTLTVLDRAVKHLKKRGAEVD
                     LGKLPFDDAKTYELLASGQTVGVFQLESQGMRDTLRKMRCGSIEEITALISLYRPGPM
                     DNIDTFVDCKFGRKPVDTLH"
BASE COUNT          227 a          377 c          412 g          221 t
ORIGIN      
        1 gtgcaaggtg tccacgggtt tgcggccgaa cttgcagtcg acgaaggtgt cgatgttgtc
       61 catcgggcct ggccgataga gcgagatcag cgcggtgatt tcctcgatgg agccacaacg
      121 catcttgcgc agggtgtcgc gcatgccctg gctttccagc tggaacacgc cgaccgtctg
      181 gcccgaggcg agaagctcgt aggtcttggc gtcgtcgaag ggcagcttgc ccagatcgac
      241 ctcggcgccg cgcttcttca ggtgctttac ggcccggtcc agcaccgtca gggtcttcag
      301 gcccaggaag tcgaacttca ccagcccggc gctctcgacc catttcatgt tgaactgggt
      361 ggcgggcaga tccgagcgcg gatccttgta aagcggggtc agctgggtaa gcggccggtc
      421 gccgatgacc acgccggcgg cgtgggtcga ggcgttgcgg aacaggcctt ccagctgcag
      481 ggctacgtca agacaagccg agacggcgcc gtcttccttc ttggcctgtt tgagccgcgg
      541 ctccagttca atggcctggg ccagggtcac cggcgcggcc ggattgttcg ggaccatctt
      601 gcaaagccgg tcaacgagac ccagcggcag ctgcatcacc cgtcctacgt cgcgcagcac
      661 ggcgcgggcc tggagcgagc cgaaggtgat gatctgggcc acacggtcgc ggccatactt
      721 ttcctgaacg tagacgatga cctcttcgcg tcgctcctga cagaagtcga tatcgaagtc
      781 gggcatcgag acccgctcgg gattcaggaa ccgctcgaac agcaggccaa agcgcagggg
      841 atcaagatcg gtaatggtca gcacccaggc gaccagcgat ccggcgcccg aaccccggcc
      901 aggaccgacg ggaatgccgt gcgacttggc ccacttgatg aagtccgaca cgatcaggaa
      961 gtagccgggg aagcccatct tgatgatgat gccgagctcg aagtccagtc ggtcccaata
     1021 gacctgctca tcgacagcca gcacgttgtc ggcgagcctg gccctgaggc cttctcgcgc
     1081 ctgataggtc agttcttcag gctcgctgcg gccggccccg gtggggaagc tgggcaggat
     1141 ggggtcgcgc ttcttgacca ggaacgcgca acgacgggcg atgtcgaggg tgttgtcgca
     1201 ggcttccgga agatccgaga acagcttgcg catgtcg
//
LOCUS       DAOMEO010001120         1237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11195_length_1237_cov_1.983080, whole genome shotgun sequence.
ACCESSION   DAOMEO010001120 DAOMEO010000000
VERSION     DAOMEO010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11195_length_1237_cov_1.983080"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLH31_18960"
     CDS             complement(<1..345)
                     /locus_tag="PLH31_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HQR91309.1"
                     /translation="MKHQLLLSAASLALVLAAAPAFAAETATPATANTEVDAVIVIGQ
                     GQSRQVQTLKDDSISLEAAGTSPLKAIDKLPGVTFQSADAFGAYEWSARISIRGFNQN
                     QLGFTLDGVPLGD"
     gene            585..>1237
                     /locus_tag="PLH31_18965"
     CDS             585..>1237
                     /locus_tag="PLH31_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR91310.1"
                     /translation="MAQQYIFQMQGLTKAFPGGKKVFENIWLSFYSDAKIGVVGVNGS
                     GKSTLLKIMAGLDDQFSGEAKAAEGIRRGYLPQEPVLDPTKDVWGNVIADCEDKQIFD
                     RYNAAANKLGEEYSDELMEEMTKLQEIIDARDLWDIDSKVEMAIDALRCPANDANIEN
                     LSGGEKRRIALARLLLSKPDIMLLDEPTNHLDAESVAWLQHHLENFPGCVILVTHDR"
BASE COUNT          234 a          403 c          393 g          207 t
ORIGIN      
        1 gtcgcccagc ggcacgccgt ccagggtgaa gcccagctgg ttctggttga agccgcggat
       61 cgagatgcgt gccgaccact cataggcgcc gaaggcgtcg gccgactgga aggtgacgcc
      121 cggcagcttg tcgatggcct tcagtgggct ggtgccggcg gcttccagcg agatcgagtc
      181 gtccttcagg gtctggacct ggcggctctg gccctggcca atcacgatca cggcgtcgac
      241 ttcggtgttg gcggtggccg gcgtggcggt ctcagcggcg aaggccggcg cggcggccag
      301 gacgagcgcc agcgaggccg cgctcagcag cagttgatgt ttcattgaat tatccccaga
      361 ccggcgctcg ggagcggcac cgtattagcg cccggttaac agcgctccgc gacgggccgg
      421 cgacgaattg gtgtcgcttg gtcgacggtc tggtgacgga atggtgaagc ttgctccacg
      481 ctgtcctgcg actgaaacgc ccgccgacat tgcgcaaacc gcccctgacc gctatttcag
      541 cgcccgtcct cccccgaact tcgacagcga gatcaacgcc ccccatggcc cagcaatata
      601 ttttccagat gcaaggcctg accaaggcgt tcccgggcgg caagaaggtg ttcgagaaca
      661 tctggctgtc gttctattcc gacgccaaga tcggcgtcgt cggcgtcaac ggttcgggta
      721 aatcgaccct cctgaagatc atggccggcc tggacgacca gttctcgggc gaggccaagg
      781 ctgccgaggg tatccgccgg ggctacctgc cgcaggagcc cgtgctggac ccgaccaagg
      841 acgtctgggg caatgtcatc gccgactgcg aggacaagca gatcttcgac cgctacaacg
      901 cggcggccaa caagctcggc gaggagtatt ccgacgagct gatggaggag atgaccaagc
      961 tccaggagat catcgacgcc cgcgacctgt gggacatcga ctccaaggtt gaaatggcca
     1021 tcgacgccct ccgctgcccg gccaacgacg ccaatatcga aaacctgtcg ggcggtgaaa
     1081 agcgccgcat cgccctggcg cgcctgctgc tcagcaagcc agacatcatg ctgctggacg
     1141 aacccaccaa ccacctcgac gccgagtctg tagcctggct gcagcaccac ctggaaaact
     1201 tcccgggctg cgtcatcctg gtgacccacg atcgcta
//
LOCUS       DAOMEO010001121         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11207_length_1236_cov_2.392041, whole genome shotgun sequence.
ACCESSION   DAOMEO010001121 DAOMEO010000000
VERSION     DAOMEO010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_1236_cov_2.392041"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PLH31_18970"
     CDS             <1..1217
                     /locus_tag="PLH31_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HQR91311.1"
                     /translation="DGDVFDYANDQAELDRQIGARNPADVEGYRRFHAYSEELYKKGY
                     LELGAEPFLDIRSMISAAPALMKLQAWRSVYDKVSSFVKDEHVRQALSFHSLLVGGSP
                     FATSSIYALIHALERRGGVWFPRGGTGALIRGLVRLLEDLGGTIRLNAPVQKITTKDG
                     RVTGVIHHDGAFEAFDTVASNADVMHTYRELLVDDPRGKKVGKALEKKRWSPSLFVIY
                     FGLKAEHPEIRHHTICFGPRFRELIGEIYNKDALADDFSLYLHSPCATDPGMAPPGCT
                     SFYVLSVVPNMETAPIDWAKEGPGYRDKILNYLEERYIPNLSRDLVTSRIFTPDDFTT
                     ELNAWNGAAFSLEPLLTQSAFFRTHNRDDVIPNLYFVGAGTHPGAGIPGVVGSAKATA
                     GLMIDDFQTRAA"
BASE COUNT          227 a          412 c          371 g          226 t
ORIGIN      
        1 aagatggcga cgtgttcgac tatgccaacg accaggccga gcttgatcgc cagatcgggg
       61 cgcgcaaccc cgctgacgtc gagggctatc gacgtttcca cgcctattcc gaagagctgt
      121 acaagaaggg ttatctcgag ctcggcgccg agccgttcct cgacatccgc agcatgatct
      181 ccgcggcgcc cgccttgatg aagctgcagg cctggcgcag cgtctatgac aaggtctcgt
      241 cgttcgtgaa ggacgagcat gtccgtcagg cgctctcgtt ccacagcctg ctggttggcg
      301 gcagcccgtt cgcgacctca tcgatctatg ccctgatcca cgccctggaa cggcgcggcg
      361 gcgtctggtt cccgcgcggc ggcacgggcg cgctgatccg cggtctggtg cgcctgctgg
      421 aagatctggg cggtacgatc cgtctcaacg ccccggtcca gaagatcacc accaaggacg
      481 gccgcgttac aggcgtgatc catcacgacg gcgcgttcga agcgttcgac accgtcgctt
      541 ccaacgccga cgtcatgcac acctatcgcg aactgctggt cgatgacccg cgtggcaaga
      601 aggtgggcaa ggccctggag aagaagcgct ggagcccctc gctgttcgtg atctatttcg
      661 gcctcaaggc tgaacacccc gaaattcgcc accacacgat ctgctttggt ccgcgcttcc
      721 gggagctgat tggcgagatc tacaacaagg acgctctggc ggacgatttc tcgctctacc
      781 tgcattcgcc ctgcgccaca gatccgggga tggccccgcc cggatgcacc agcttctacg
      841 tcctgtccgt ggtgccgaat atggaaactg ccccgatcga ctgggccaag gaagggcccg
      901 gctatcgcga caagatcctg aactatctgg aagagcgcta catccccaac ctgtcgcgcg
      961 atctggtcac cagccgcatc ttcacgccag acgatttcac gaccgagctc aacgcctgga
     1021 acggcgcggc cttctcgctg gaaccgctgc tgacccagag cgccttcttc cgcacccaca
     1081 atcgcgatga cgtgatcccg aacctctatt tcgtcggcgc tggcacccat ccgggggcgg
     1141 gcattccggg cgtggtcggt tcggccaagg ccacggccgg cttgatgatc gacgatttcc
     1201 agacgcgggc ggcgtgagcg acctagaggc gcaaag
//
LOCUS       DAOMEO010001122         1236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11211_length_1236_cov_1.652837, whole genome shotgun sequence.
ACCESSION   DAOMEO010001122 DAOMEO010000000
VERSION     DAOMEO010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_1236_cov_1.652837"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLH31_18975"
     CDS             complement(<1..257)
                     /locus_tag="PLH31_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91312.1"
                     /translation="MNAIIRGKPDNLDEIGLRFDRATLTTPVYLNSVPKAGTHLIRNI
                     LRMFVAPEQHWRREYIQHAILRQYGPEAFSTAKPMISWGHML"
     gene            complement(345..>1236)
                     /locus_tag="PLH31_18980"
     CDS             complement(345..>1236)
                     /locus_tag="PLH31_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91313.1"
                     /translation="QNLAQGNITQNVADNCAADGIPSTHTGAGSSATVTSGGGKGALE
                     AETSKAVTYGVIWTPSFADLNVAVDYFEIEVNDEITRLGAARIVAGCYRSLTFATDPL
                     CNLFTRTPGSNLINSVTNNYLNIAEQANRGIDLTVRYGQELPWDSKLTVDLQTTWQLE
                     DTTALFAGTTIDENGFVGDPDWTGQLNFRLTKGDWTGFWGIDMVGKASDAEDYADKNA
                     AGTTFYKIHTEFTAYHSLSLQKKFDDWSILGGVANVFSEEPPAVSLGQGNYSTVGSSV
                     LASQYDYFGRRLFVNITARF"
BASE COUNT          218 a          374 c          408 g          236 t
ORIGIN      
        1 agcatgtgcc cccagctgat catcggcttg gcggtcgaaa acgcctccgg cccatactgg
       61 cgcagaatgg cgtgctggat gtattcgcgc cgccagtgct gctcgggcgc gacgaacatc
      121 cgcaggatat tgcggatcag gtgggtgccg gccttgggta cgctgttcag atagaccggc
      181 gtcgtcagcg tcgcccgatc aaagcgcagg ccgatctcgt ccaggttgtc gggtttgccc
      241 cgaatgatgg cgttcacgcg agcaggttcc ccgacacaga tggcgataca gaaacggcgg
      301 cgggtcgccc cgccgccgtc atgttagtcc aagagtctaa gcgactagaa gcgcgccgtg
      361 atgttcacga acaggcggcg gccgaaatag tcatactgcg acgccaggac cgaggaaccc
      421 accgtgctgt agttgccctg gcccagcgac acagccgggg gctcctcgct gaacacgttg
      481 gccacaccgc ccaggatcga ccagtcgtcg aacttctttt gcagcgacag gctgtgatag
      541 gcggtgaact cggtgtggat cttgtagaag gtcgtgccgg cggcgttctt gtcggcatag
      601 tcttcggcgt cggacgcctt gccaaccatg tcgatgcccc agaaaccggt ccagtcgccc
      661 ttggtcaggc ggaagttcaa ctggccggtc cagtcgggat caccgacaaa gccgttctcg
      721 tcgatcgtcg tgccggcgaa cagagcggtg gtgtcctcca gctgccaggt cgtctgcaga
      781 tcgaccgtca gcttggagtc ccagggaagc tcctggccat agcgaaccgt caggtcgatg
      841 ccgcggttgg cctgctcggc gatgttgaga tagttgttgg tgacgctgtt gatcaggttc
      901 gagcccgggg tgcgggtgaa cagattgcac agcggatcgg tcgcgaaggt cagcgagcga
      961 tagcagcccg cgacgatccg cgcggcgccc aggcgcgtga tttcgtcgtt cacttcgatc
     1021 tcgaagtagt cgacggccac gttcagatcc gcaaacgacg gcgtccagat caccccgtag
     1081 gtcacggcct tggaggtctc tgcctcaaga gcgcccttgc cgccgccgga ggtgacggtc
     1141 gccgacgagc ctgcacccgt gtgggtggac ggaatgccgt cagccgcgca gttgtcggcg
     1201 acattctgcg tgatgttgcc ctgcgccagg ttctgg
//
LOCUS       DAOMEO010001123         1231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11266_length_1231_cov_3.218537, whole genome shotgun sequence.
ACCESSION   DAOMEO010001123 DAOMEO010000000
VERSION     DAOMEO010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11266_length_1231_cov_3.218537"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLH31_18985"
     CDS             <1..759
                     /locus_tag="PLH31_18985"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQR91314.1"
                     /translation="RRTAQLLDAQRLADGCGRPVAFDFRTADVAAGGEGAPLAPIYHA
                     ARARASGLAAPVAALNVGGVANITLIDADGTLLAFDTGPGNGMIDLMLQGRDLGRFDE
                     NGRLAAAGTVDEAALRMLLSGAYFDASAPKSLDRYDFTLDAVEHLSAEDAAATLVAFT
                     AEAVVKAFDKGARPSALIVCGGGRHNPEIMRVLTARAPVPVMTAEAVDWRGDAIEAEA
                     FAYLAARTALGLPISFPGTTGVAAPMTGGRIVQP"
     gene            complement(797..>1231)
                     /locus_tag="PLH31_18990"
     CDS             complement(797..>1231)
                     /locus_tag="PLH31_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR91315.1"
                     /translation="GEFDAGIGELADAATALDWLQTSNPAASQTWVAGFDFGAYIGMQ
                     LLMRRPETDGFISVSPPTNMYDFSFLAPCPASGLFLTGSADSITPPVEVERVVSKLRT
                     QKGIIIDYEVIDKASHFWTEHLPFVEKSVGDYLDKRIEMNPI"
BASE COUNT          188 a          416 c          424 g          203 t
ORIGIN      
        1 cgccgcaccg cacaactgct ggacgcccaa cgcctggccg acggctgcgg ccgtccggtc
       61 gccttcgact tccgcaccgc cgatgtcgcg gcgggtgggg agggcgctcc gctggccccg
      121 atctatcacg cggcccgcgc tcgggcttcg ggtctggccg cgccggtggc ggcgctgaac
      181 gtcggcggcg tggccaacat caccctgatc gacgccgatg gaacgctcct ggccttcgac
      241 accggccccg gcaacggcat gatcgacctg atgctgcagg gccgcgatct gggccgcttc
      301 gacgagaatg gacgactggc cgcggccggg acggtcgatg aggccgcgct ccggatgctg
      361 ctgtcgggag cctatttcga cgcgtctgcg cccaagtctc tggatcgcta cgatttcacg
      421 ctcgatgcgg tcgagcacct gtcggccgag gacgccgccg ccaccctagt ggccttcacc
      481 gccgaagccg tggtcaaggc gttcgacaag ggcgcgcgac cgagcgccct gatcgtctgc
      541 ggcggcggcc ggcacaatcc cgagatcatg cgcgtactga ccgcgcgggc tcccgtcccg
      601 gtcatgaccg ccgaggccgt cgactggcgc ggcgacgcga tcgaggctga ggctttcgct
      661 tatctggcgg cgcgcaccgc gttaggcttg ccgatcagct tcccgggcac cacgggcgtc
      721 gccgcgccga tgaccggcgg gcgcatcgtt caaccctgaa ctaaaaaggc ctccccgaag
      781 ggaggccttt gaacgctcag atcgggttca tctcgatccg cttgtccaga tagtcgccga
      841 cgctcttttc cacgaagggt aggtgctcgg tccagaagtg gctggccttg tcgatgactt
      901 cgtagtcgat gatgatgccc ttctgggtgc gcagcttgga gaccacgcgc tcgacttcga
      961 ccggcggggt gatgctgtca gccgagcccg tcaggaacag gcccgaggcc gggcagggcg
     1021 ccaggaagct gaaatcgtac atgttggtag gcggcgagac gctgatgaaa ccatccgtct
     1081 ccgggcggcg catcagcagt tgcatgccga tataggcgcc aaagtcgaag ccggcgaccc
     1141 aggtctggct ggcggccggg ttggaggtct gcagccagtc cagcgcggtg gcggcgtcgg
     1201 ccagctcgcc gataccggcg tcgaactcgc c
//
LOCUS       DAOMEO010001124         1228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11306_length_1228_cov_2.078431, whole genome shotgun sequence.
ACCESSION   DAOMEO010001124 DAOMEO010000000
VERSION     DAOMEO010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11306_length_1228_cov_2.078431"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLH31_18995"
     CDS             complement(<1..578)
                     /locus_tag="PLH31_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQR91316.1"
                     /translation="MSMTHLFAAAAAVALMSGAAFAQNAPAVTGQAAAPAPAAASPVV
                     AKGDLIETATASGQFSIFLKAVSSVNLTSVLKTNQNLTLFAPTDAAFAALPAGELEKL
                     MLPDNGPMLQKVLTYHLINTKVDSSKIKGAKGEVKTVQGSSVMLDGSGDTAMVDGATI
                     VQADVMATNGVLHVVDKVLMPKDMPGAQAAAAT"
     gene            complement(664..1014)
                     /locus_tag="PLH31_19000"
     CDS             complement(664..1014)
                     /locus_tag="PLH31_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQR91317.1"
                     /translation="MIAAWPKFAPWLLLIASNVFMTFAWYGHLKNKSLTLPLAILSSW
                     LIALPEYMLAVPANRLGHQVYSTAQLTTGQEAITLIVFTLFSYFYLGEALKWTTFLGF
                     ALIIAGAALVFFFR"
BASE COUNT          224 a          376 c          404 g          224 t
ORIGIN      
        1 gtggcggcgg cggcctgagc gcccggcata tccttgggca tcaacacctt gtcgaccacg
       61 tgcaggacgc cgttggtggc catgacgtcg gcctggacga tggtggcgcc gtcgaccatc
      121 gccgtgtcac cgctgccgtc cagcatgacc gacgagccct ggacggtttt cacttcgccc
      181 ttggcgccct tgatcttgga cgagtccacc ttggtgttga tcaggtgata ggtcagcacc
      241 ttctgcagca tcgggccatt gtcgggcagc atcagctttt caagctcgcc ggccggcagg
      301 gccgcaaagg ccgcgtcggt cggcgcgaac agggtcaggt tctgattggt cttcagcacg
      361 ctggtcaggt tcaccgacga gacggccttc agaaagatgc tgaactggcc cgacgccgtg
      421 gcggtttcaa tcaggtcacc cttggcgaca acaggcgatg cggcggctgg cgcaggcgct
      481 gcggcctggc cggtcacggc gggcgcgttc tgggcgaaag ccgcgcccga catcagggcg
      541 acggcggcgg cggcagcgaa aaggtgggtc atcgacatgg cgaggttcct cgtgttccag
      601 gctgcctcta cgcttggccc ccggcagagg ttgccgacga tcacgggctc gtgaacacct
      661 gccctacctg aaaaagaaca ccagggcggc ccctgcgatg atcagggcga agcccaggaa
      721 ggtcgtccat ttcagcgctt cgcccaggta gaaatacgag aacaaggtga acacaatcag
      781 ggtgatagcc tcctgccccg tcgtcagctg ggcggtggaa tagacctgat gacctagccg
      841 gttggcgggc acggccagca tgtattccgg cagggcgatc agccagctgc tcagaatggc
      901 cagcggcagg gtcaggctct tgttcttcag gtgaccgtac caggcgaacg tcatgaacac
      961 gttcgaggcg atcagcagca gccagggtgc gaacttgggc catgcagcga tcatgggtcg
     1021 accttcctga gggtgggcgg gattccctca ggctagagca acagcggctt tgaggaccag
     1081 agcccggggc tgcgcaaaca tcgccgtttt atgacgcagt gacgacgggc tttgcctgaa
     1141 tcttgggcgc cttgcccatt tcctttccaa atccctattg cgcgaccgca cgtcgcggac
     1201 gtcccccgat agcgtaacca tcgccggg
//
LOCUS       DAOMEO010001125         1228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11311_length_1228_cov_1.595055, whole genome shotgun sequence.
ACCESSION   DAOMEO010001125 DAOMEO010000000
VERSION     DAOMEO010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11311_length_1228_cov_1.595055"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>401
                     /locus_tag="PLH31_19005"
     CDS             <1..>401
                     /locus_tag="PLH31_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQR91318.1"
                     /translation="GEKAGKVTGVRILGATTPQEEEFYADVVFLCASAFNSAWIMMNS
                     TSSRFPNGFGNGSDQLGRNVMDHHLGVGASGEAPEFADQYYSGRRPNGIYVPRFRNLG
                     DAATKRTDYLRGFGYQGGASRAGWDRDLGRR"
     assembly_gap    402..411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <412..918
                     /locus_tag="PLH31_19010"
     CDS             <412..918
                     /locus_tag="PLH31_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HQR91319.1"
                     /translation="VMGHGKCGGCKAALSQAGPWTIGLGGFGEILPYEDNRLTLNHET
                     KDVFGLPTLSINVTMRENEIAMRRDMQTAAAEMLEAAGFKNVKGRDGGYAPGLGIHEM
                     GTARMGRDPKTSVLNAHNQVHECKNVYVTDGAAMTSASCVNPSLTYMALTARAVDHAV
                     QARKRGDL"
     gene            918..>1228
                     /locus_tag="PLH31_19015"
     CDS             918..>1228
                     /locus_tag="PLH31_19015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HQR91320.1"
                     /translation="MLNRRDTLRGLAPTIGVGASGWAERALAAAAPAPALTWTPTALT
                     PDQARVLDVVAELIIPKTDTPGAREAGVPQFIDRALANYYEKDQADRLRAGLTRMDAD
                     A"
BASE COUNT          217 a          408 c          409 g          184 t
ORIGIN      
        1 atggcgagaa ggcgggcaag gtgacgggcg tgcgcatcct gggcgcgaca acgccccagg
       61 aagaggagtt ctatgctgac gtggtgttcc tgtgcgcctc ggccttcaac tcggcctgga
      121 tcatgatgaa ctcgaccagc tcgcggttcc ccaacggctt tggcaatggc agcgaccagc
      181 tgggccgaaa cgtgatggac caccatctgg gcgtgggcgc atcgggcgag gcgcccgagt
      241 ttgccgacca gtactattcc ggccgccgac ccaacggcat ctatgtgccg cgcttccgca
      301 acctgggtga cgcggccacc aagcgcaccg actatctgcg aggcttcggc tatcagggcg
      361 gcgcatctcg cgcgggctgg gaccgcgacc tgggccggcg cnnnnnnnnn ngtcatgggt
      421 catggcaaat gcgggggttg caaggcggcg ctcagtcagg ccgggccctg gaccattggc
      481 ctgggcggct ttggcgaaat cctcccctac gaggacaacc gcctgaccct gaaccacgag
      541 accaaggacg tcttcggcct gccgaccctg tcgatcaatg tcaccatgcg cgagaacgag
      601 atcgccatgc gccgcgacat gcaaacggcg gcggccgaga tgctggaggc ggcgggcttc
      661 aagaacgtca agggtcgcga tggcggctac gcgcctggcc ttggcatcca tgaaatgggc
      721 accgcgcgca tgggccggga tcccaagacc tcggtgttga acgcccacaa ccaggtgcat
      781 gagtgcaaga acgtctatgt caccgacggc gcagccatga cctcggcgtc ctgcgtgaac
      841 ccgtccctga cctatatggc cctgaccgca cgcgcggtcg atcacgccgt ccaggcgcgc
      901 aagcggggag acctgtgatg ctgaaccgac gcgacaccct tcggggcctg gccccgacga
      961 tcggggttgg cgcctcaggc tgggccgagc gcgcccttgc ggccgccgcg ccagcgccgg
     1021 ccctgacctg gacccccaca gctttgacgc ccgatcaggc ccgcgtgctg gatgtggtgg
     1081 ccgaactgat cattccgaag acggacacgc ccggcgcccg cgaggccggg gtgccccagt
     1141 tcatcgaccg ggccctggcc aactattacg agaaggatca agccgataga ttgcgggccg
     1201 gtctgacgcg catggacgcc gacgccca
//
LOCUS       DAOMEO010001126         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11338_length_1226_cov_1.802733, whole genome shotgun sequence.
ACCESSION   DAOMEO010001126 DAOMEO010000000
VERSION     DAOMEO010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11338_length_1226_cov_1.802733"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1226)
                     /locus_tag="PLH31_19020"
     CDS             complement(<1..>1226)
                     /locus_tag="PLH31_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQR91321.1"
                     /translation="KTVVAKGVAVAMPELAHAVHVEVGGLEPGRDYWYRFVAGRERSL
                     TGRARTTPAQGATVERVRFAVAGCQNYEQGYYTAHRRLAEENPDFVFCYGDYIYEGRG
                     SRVWNSARGPVENVRQHLGGEIYSLDDYRRRYALYKMDADLQAAHAAAPWFVVWDDHE
                     TDNNWVADLDQDGVDPKIFNLRRQAAVQAYFENMPLRPSSFPIGTTIQLYRRASFGGL
                     MDLNLLDTRQYRSNQPCGDKFGTCAEIEAAKAEVLGLKQEKWLLDGLSASKARWNVLA
                     QQIMVMDLDRDPGAGSAVNPDSWAGYRTPRNRLLKQIRDRRIANPIVLTGDEHQNYAG
                     ELHLDGANPEGAPIGTEFVATSISSGGDGVDQREDMARIQAVNPQLKFNNSQRGYVIC
                     DVTPERWTTEFKVLDA"
BASE COUNT          188 a          404 c          410 g          224 t
ORIGIN      
        1 tggcgtccag caccttgaac tcggtggtcc agcgctcggg cgtcacgtcg cagatcacat
       61 agccgcgctg gctgttgttg aacttcagct gcggattgac ggcctggatc ctggccatgt
      121 cctcgcgctg atcgacgccg tcgccgcccg acgagatcga ggtggccacg aactcggtcc
      181 cgatcggcgc gccctcggga ttggcgccgt ccagatgcag ctcgccggca tagttctggt
      241 gctcgtcgcc ggtcaggacg atggggttgg cgatcctgcg gtcgcggatc tgtttgagca
      301 ggcgattgcg cggcgtgcga tagcccgccc aggaatccgg attgaccgcc gagcccgcgc
      361 cgggatcacg atccaggtcc atgaccatga tctgctgggc cagcacgttc cagcgcgcct
      421 tggaggccga aaggccgtcg agcagccact tttcctgctt cagacccagc acctcggcct
      481 tggctgcttc gatctcggcg caggtcccga acttgtcgcc gcacggctga ttgctgcgat
      541 actggcgggt gtcgagcagg ttcagatcca tcagtccgcc gaagctggcg cgacgataga
      601 gctggatcgt cgtaccgatc gggaagctgc tgggccgcag cggcatgttc tcgaaatagg
      661 cctggacggc ggcctgccgg cgcaggttga agatcttggg atcgacaccg tcctggtcga
      721 ggtccgcgac ccagttgttg tcggtctcgt ggtcgtccca gaccacgaac cagggcgcgg
      781 cggcgtgggc ggcctgcagg tcggcgtcca tcttgtagag ggcgtagcgg cggcgatagt
      841 cgtccaggct atagatctcg ccgcccagat gctggcgaac gttctcgacc ggtccccggg
      901 cgctgttcca gacgcggctt ccgcgacctt catagatgta gtcgccatag cagaacacga
      961 agtcgggatt ctcctcggcc aggcggcggt gggcggtgta atagccctgc tcatagttct
     1021 ggcagccggc cacggcgaag cgcacccgct ccacggtcgc gccctgcgcc ggcgtcgtac
     1081 gtgcgcggcc ggtcaggcta cgctcccggc ccgccacaaa gcgataccag tagtcgcggc
     1141 cgggctcgag accgcccacc tcgacatgca cggcgtgcgc cagttcgggc atcgccacgg
     1201 ccacgccctt ggcgaccacg gtttta
//
LOCUS       DAOMEO010001127         1225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11343_length_1225_cov_2.481197, whole genome shotgun sequence.
ACCESSION   DAOMEO010001127 DAOMEO010000000
VERSION     DAOMEO010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11343_length_1225_cov_2.481197"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..906
                     /locus_tag="PLH31_19025"
     CDS             31..906
                     /locus_tag="PLH31_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQR91322.1"
                     /translation="MALPVAAQAADIKMAITVDDLPAHSALPTGETRVSIARDFLAAL
                     KEAKAAPIYGFINGVQTEREPGSAQVLKIWRDAGHPLGNHGWAHLNLAQIDVATFEAE
                     LTRNEPLLKSLAAGSEWRWFRFPFLSEGDTPEKRAAVRELLKARGYRIASVTTSFGDY
                     AWNEPYARCLAKGDHKAIADLETSFLKAAELSLAHSRALSATLHGQDIPYVLLMHLGA
                     FDARMGPRLLALYKSLGVSFVSLQEAQAHPFYAADLTASPAAAPVTFESAMTAKGLPV
                     PPLSLNLAALDGVCR"
     gene            962..>1225
                     /locus_tag="PLH31_19030"
     CDS             962..>1225
                     /locus_tag="PLH31_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQR91323.1"
                     /translation="MSQASFDGMIALPGGVFQMGSDEHYPEEAPAHPVAVDPFWIDET
                     PVTNAQFARFVAATGHVTLAEIPPDPKLYPGMDPAFAHPASAVF"
BASE COUNT          197 a          460 c          374 g          194 t
ORIGIN      
        1 atcctgcgcg ccctgctcgc cgcctgcctg atggccctgc ccgtcgccgc ccaggcggcc
       61 gacatcaaga tggcgatcac ggtcgacgac ctgccggccc acagcgccct gccgactggc
      121 gagacccgcg tcagcatcgc cagggacttc ctcgccgccc tgaaggaggc caaggccgcc
      181 ccgatttatg gcttcatcaa cggcgtccag accgagcgcg agcccggctc ggcgcaggtg
      241 ctgaagatct ggcgcgacgc cggccacccg ctgggcaatc acggctgggc gcacctgaac
      301 ctggcccaga tcgacgttgc gacctttgag gccgaactca cccgcaacga accgctgctg
      361 aagagcctcg ccgccggatc cgaatggcgc tggttccgct tccccttcct cagcgagggc
      421 gacacgccgg agaagcgcgc cgctgtccgc gagttgctga aggcccgcgg ctaccgcatc
      481 gccagcgtga ccacgagctt tggcgactat gcctggaacg agccctatgc ccgctgcctg
      541 gccaagggcg accacaaggc catcgccgac ctcgaaacca gcttcctgaa ggccgccgaa
      601 ctgagcctgg cccattcgcg cgccctgtcg gcgacgctgc atggccagga catcccgtat
      661 gtgctgctga tgcacctggg cgcgttcgac gcccgcatgg ggccgcgcct gctggctctc
      721 tacaagtcgc tgggcgtcag tttcgtctcg ctgcaggagg cccaggcgca tccgttctat
      781 gcggccgacc tcaccgcctc gccagccgcc gcgcccgtca ccttcgagag cgctatgacg
      841 gccaagggcc tgcccgttcc gcccttgtcg ctgaacctcg cggctctgga cggggtctgc
      901 cgctagaaaa ctagtggcgc gaggcgtgac aacatccctc gcgacgccct agaactgggg
      961 gatgagccag gcttccttcg acggcatgat cgccctgccc ggcggagtct tccagatggg
     1021 ttcagacgaa cactatccgg aggaggcccc ggcccatccc gtggccgtcg acccgttctg
     1081 gatcgacgaa acgccagtca ccaacgccca gttcgcacgg ttcgtcgcag cgacgggtca
     1141 tgtgaccctg gccgagatcc cgcccgatcc aaagctctat cccggcatgg acccggcctt
     1201 cgcccatccg gcctcggcgg tattc
//
LOCUS       DAOMEO010001128         1223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11369_length_1223_cov_2.186644, whole genome shotgun sequence.
ACCESSION   DAOMEO010001128 DAOMEO010000000
VERSION     DAOMEO010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11369_length_1223_cov_2.186644"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLH31_19035"
     CDS             <1..221
                     /locus_tag="PLH31_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQR91324.1"
                     /translation="DLRLNEPRYASLPNIMKAKKKEIVSKTVADYGVDIAPRLKVLKV
                     TEPAKRSAGIKVETAADLVSKLNTAGVL"
     gene            222..1163
                     /locus_tag="PLH31_19040"
     CDS             222..1163
                     /locus_tag="PLH31_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQR91325.1"
                     /translation="MAVLVVADNDNAHLRDGTHKTVTAALKLSGDVDVLVLGHGAKAV
                     ADAAAKISGVRKVLLADSAALGHGVAEAQADAVLALAGNYDAILVPATSGGKNFAPRV
                     AAKLDVAPISEIVEVVSADTFVRPIYAGNALETVQSSDAKKVITVRPTAFAAAEEGGS
                     APVENASGADADKTRFVSEEMVKSDRPELAAAKIVVSGGRAMGSAEEFQKVIEPLADK
                     LGAAVGASRAAVDAGYAPNDYQVGQTGKVVAPALYVAIGISGAIQHLAGMKDSKIIVA
                     INKDSDAPIFQVADFGLVADYKTAVPELMDALAAAGK"
BASE COUNT          220 a          427 c          398 g          178 t
ORIGIN      
        1 ccgatctccg cctcaatgag ccgcgctacg cctcgctgcc caacatcatg aaggccaaga
       61 agaaggagat cgtcagcaag acggtcgccg actacggcgt cgacatcgcg ccgcgcctga
      121 aggtcctgaa ggtcaccgag cccgccaagc gctcggccgg catcaaggtg gaaaccgccg
      181 ccgacctcgt ttccaagctc aacactgcgg gagtgctcta gatggctgtt ctcgtcgtcg
      241 ccgataacga caacgcgcac ctgcgcgatg gcacccacaa gaccgtcacc gccgccctga
      301 aactgtcggg cgacgtggac gtgctggtcc tgggtcacgg cgccaaggcc gtggccgatg
      361 cggccgccaa gatcagcggc gtccgcaagg tgctgctggc cgacagcgcc gccctgggtc
      421 atggcgtggc cgaagcgcaa gccgacgccg tgctggccct ggccggcaac tacgacgcga
      481 tcctcgtgcc ggccacctcg ggcggcaaga acttcgcgcc gcgcgtcgcc gccaagctgg
      541 acgtcgcccc gatcagcgag atcgtggaag tcgtctcggc cgacaccttc gtgcgtccga
      601 tctatgccgg caacgccctc gaaaccgtcc agtcgtcgga cgccaagaag gtgatcaccg
      661 tccgtcccac cgccttcgcg gcggcggaag aaggcggctc ggccccggtc gagaacgcct
      721 ccggcgcgga cgccgacaag acccgcttcg tcagcgaaga gatggtcaag tcggaccgtc
      781 cggaactggc tgcggccaag atcgtcgtct cgggcggtcg cgccatgggc tcggccgaag
      841 agttccagaa ggtgatcgag cccctggccg acaagctggg cgccgccgtc ggcgcctcgc
      901 gcgcggcggt cgatgcgggc tacgccccca acgactatca ggtcggtcag accggcaagg
      961 tggtggctcc ggccctgtat gtcgccatcg gcatctcggg cgccatccag cacctggccg
     1021 gcatgaagga cagcaagatc atcgtcgcca tcaacaagga cagcgacgcc ccgatcttcc
     1081 aggtcgccga tttcggcctg gtggctgact acaagaccgc tgtgccggaa ctgatggacg
     1141 cgctggcggc cgccgggaag taggcttgcg cccacaagac cctgaaaagg cccggaggcg
     1201 actccgggcc tttttgtttg ggc
//
LOCUS       DAOMEO010001129         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11379_length_1222_cov_2.989717, whole genome shotgun sequence.
ACCESSION   DAOMEO010001129 DAOMEO010000000
VERSION     DAOMEO010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11379_length_1222_cov_2.989717"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLH31_19045"
     CDS             <1..740
                     /locus_tag="PLH31_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQR91326.1"
                     /translation="ALVVVRKVLSWVIRLTPIGTIGLLGNAVAQYGWTTLGQLGAFTG
                     AIYVGLGLVLFVVYPVLLAANGLNPLKFFAGAWPAIQLGFVSRSSVGTLPVTEAVTEQ
                     SLGVPRAYSAFAVPLGATTKMDGCAAIYPAISAIFVAQFFGLQLGLSDYFLIVFVSVI
                     GSAATAGLTGATVMLTLTLSTLGLPLEGVGLLLAIDPILDMGRTAVNVAGQALVPTIV
                     AKREGILDLEVYNAGRTPADPAIQAAE"
     gene            808..>1222
                     /locus_tag="PLH31_19050"
     CDS             808..>1222
                     /locus_tag="PLH31_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQR91327.1"
                     /translation="MVSKASEGGRFGPSRRSMLAGAVALAGFSVTAPARAASRASDRI
                     EALLARMTIEEKAGQLSCFADMIRPPIGDINPQVNIRNAQTLLAETRAGRIGTLMNGL
                     GTQGALDAQKAAVEGSRLKIPLLFAADVIHGFRTVF"
BASE COUNT          163 a          424 c          410 g          225 t
ORIGIN      
        1 cggcgttggt tgtcgtgcgc aaggtactat cgtgggtgat ccgcctgacg ccgatcggca
       61 ccatcggcct gctgggcaac gccgtggcgc agtatggctg gaccacgctg ggccagctgg
      121 gggcctttac cggcgcgatc tatgtcggcc tgggcctggt tctgttcgtc gtctacccgg
      181 tcctgctcgc cgccaacggc ctgaacccgt tgaagttctt cgccggcgcc tggcctgcga
      241 tccagctggg cttcgtctcg cgttcgtcgg tcggcaccct gccggtgacc gaagccgtga
      301 ccgagcagag cctgggcgtg ccgcgcgcct attcggcttt tgccgtgccg ctgggcgcga
      361 ccaccaagat ggatggctgc gcggccatct atccggccat ctcggcgatc ttcgtggccc
      421 aattctttgg cctgcagctg ggcctatcgg actatttcct gatcgtcttc gtgtcggtga
      481 tcggctcggc cgccacggcg ggcctcaccg gcgcgaccgt gatgctgacc ctgaccctgt
      541 cgacgctagg cctgccgctg gagggtgtgg gtctcctgct agccatcgac ccgatcctcg
      601 acatgggccg cacggccgtc aacgtcgcgg gccaggctct ggtgccgacc atcgtcgcca
      661 agcgcgaggg cattcttgac cttgaggtct acaacgccgg ccgcacgccc gccgacccgg
      721 cgatccaggc cgccgaatag gcgagcgcgg ttgcgccttt tgcctttagc ggcgaggttg
      781 ccggcttgag gcgcacaaag ggaatcgatg gtgtcgaagg cgagtgaagg cggccggttc
      841 gggccgagcc ggcggtccat gctcgccggg gccgtagccc tcgccgggtt ctccgtcacg
      901 gccccggccc gcgcggccag ccgggcgtcc gaccggatcg aggcgcttct cgcccgcatg
      961 accatcgagg agaaggccgg ccagctgtcg tgcttcgccg acatgatccg cccgccgatc
     1021 ggcgacatca atccgcaggt caatatccgc aacgcccaga ccctgctggc cgagacccgc
     1081 gccgggcgta tcggtactct gatgaacggc ctgggcacgc aaggcgcgct cgacgcccag
     1141 aaggccgctg tcgaaggctc gcggctgaag attccgctgc tgttcgctgc cgacgtgatc
     1201 cacggcttcc gcacggtgtt cc
//
LOCUS       DAOMEO010001130         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11391_length_1221_cov_2.890223, whole genome shotgun sequence.
ACCESSION   DAOMEO010001130 DAOMEO010000000
VERSION     DAOMEO010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11391_length_1221_cov_2.890223"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1221)
                     /locus_tag="PLH31_19055"
     CDS             complement(<1..>1221)
                     /locus_tag="PLH31_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91328.1"
                     /translation="EAGVLARLAGDNHVRVAPDTEYRLKARAPEPLNALRPVVIGAGP
                     CGLFAGLILAQMGFKPIILDRGKVVRERTKDTWGLWRKSELNPESNVQFGEGGAGTFS
                     DGKLYSQIKDPRHLGRKVLEEFVKAGAPAEILTEAHPHIGTFRLVTMVESMRETIEAL
                     GGEYRWQHRVDELDIETAADGSRRVKGLKLSTGDYLEADRVVLAVGHSARDTFQRLYD
                     QGVHIEAKPFSVGVRIEHPQSWMDRARFGSHAGHPDLGAADYSLSHHCKNGRTVYSFC
                     MCPGGTVVAATSEPGRVVTNGMSQYSRNERNANSGFVVGIDPERDYPGHPLAGIAFQR
                     KWESLAYVAGGSTYAAPAQKVGDFLAGRPSTDLGEVAPSYKPGITMTDLSACLPAFAV
                     EAMREALPVFGRQI"
BASE COUNT          191 a          423 c          411 g          196 t
ORIGIN      
        1 cgatctggcg gccaaacacc ggcagggcct cgcgcatggc ctcgaccgcg aaggccggca
       61 ggcaggccga caggtcggtc atggtgatgc ccggcttata cgaaggcgcc acctcgccca
      121 gatcggtcga ggggcggccg gccaggaagt cgccgacctt ctgggccggc gcggcatagg
      181 tcgagccgcc ggccacatag gccaggcttt cccatttgcg ctggaacgcg atgccggcca
      241 gcggatggcc cggatagtcg cgctccgggt cgatgccgac cacgaagccg gaattggcgt
      301 tgcgctcatt gcgcgaatac tggctcatgc cgttggtcac cacgcggccc ggctccgagg
      361 tcgccgccac caccgtgccg cccgggcaca tgcagaagct atagaccgtg cggccgttct
      421 tgcagtggtg cgacaggctg tagtcggccg cgccgaggtc cggatggcca gcatgactgc
      481 caaaccgcgc ccgatccatc catgactgcg gatgctcgat ccgcaccccg acagagaacg
      541 gcttggcctc gatatggacg ccctggtcgt agagcctctg gaaggtgtcg cgggcgctgt
      601 ggccgacggc cagaaccacc cggtcggcct ccagatagtc gccggtcgag agcttcagac
      661 ccttcaccct gcgcgagccg tcggcggcgg tctcgatgtc gagctcgtcg acccggtgct
      721 gccagcgata ttccccgcct agggcctcga tggtctcgcg catggactca accatggtca
      781 ccagccggaa ggtgccgatg tgggggtggg cctcggtcag gatctcggcg ggggcgccgg
      841 ccttaacgaa ttcctccaga accttgcggc ccagatgacg ggggtccttg atctggctgt
      901 agagcttgcc gtccgaaaac gtgcccgccc cgccctcgcc gaactggacg ttggactcgg
      961 ggttcagctc gctcttgcgc cacaggcccc aggtgtcctt ggtccgctcg cgcaccacct
     1021 tgccccggtc caggatgatc ggcttgaagc ccatctgggc caggatcagg ccggcgaaca
     1081 aaccgcaggg gcctgccccg atcaccaccg gacgcagggc gttcagaggc tcgggcgcgc
     1141 gggccttcag gcgatattcg gtgtccggcg ctacgcgcac atggttgtcg ccggccaggc
     1201 gcgccagcac cccggcctcg t
//
LOCUS       DAOMEO010001131         1219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11422_length_1219_cov_1.970790, whole genome shotgun sequence.
ACCESSION   DAOMEO010001131 DAOMEO010000000
VERSION     DAOMEO010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_1219_cov_1.970790"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLH31_19060"
     CDS             complement(<1..544)
                     /locus_tag="PLH31_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQR91329.1"
                     /translation="MRLIERYLFRQLLGPTLFAAAALAALALLARSLSEFDILVTQRQ
                     SALVFLKIIMLALPQLLNIVLPIALFVAALVALNRLHTEQEIVVCFSGGMSRWRVISP
                     AMRLAVFAALFSLFIGLWVQPWAARQIRETAFAVKTDVASTLVQPGQFTEPGPGLTVY
                     AQSIDRDNLIHNLFIHQERPD"
     gene            790..>1219
                     /locus_tag="PLH31_19065"
     CDS             790..>1219
                     /locus_tag="PLH31_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HQR91330.1"
                     /translation="MRIEFVPADLHAGATAAVAVLAHEAAALSPEARVIDEATGGALA
                     RAIAGGRFTGAKGQILDLVAPHGLEAARVVLVGVDGVGAVEPEAVEAAAAHAFQAVKA
                     SGVTELVLKLGADAETAARAAFGVKLAAYRFDKYRTTEKTE"
BASE COUNT          219 a          390 c          431 g          179 t
ORIGIN      
        1 cgtccgggcg ctcctggtga atgaacaggt tgtggatcag gttatcgcgg tcgatcgact
       61 gggcgtagac ggtcagaccg gggcctggtt ccgtgaactg tccgggctgg accagggtcg
      121 aggccacatc ggtcttcacc gcgaaggcgg tctcgcgaat ttgacgggcg gcccagggct
      181 ggacccatag cccgatgaac agagagaaca gggccgcgaa caccgccagt cgcatcgccg
      241 gcgagatcac ccgccagcga ctcatgccgc ccgagaaaca gaccacgatc tcctgctcgg
      301 tgtgcagccg gttcagggcc accagggccg cgacgaacag ggcgatgggc aggacgatat
      361 tgagcagctg gggcagggcc agcatgatga tcttgaggaa aaccagcgcg ctctggcgct
      421 gggtgaccag gatgtcgaac tccgacaggc tccgcgccag cagcgcaagc gccgctaggg
      481 cggccgccgc aaagagcgtc ggacccagca actggcggaa gagatagcgt tcgatcaggc
      541 gcattaaaaa gggggttcgg gcgggtggat tcgtcaagcg cgaaccccag ggaagagcgc
      601 tgttctacac gggcgcttcg ctaccgcaca accgaagaca gcgccctgtg cgacgccgga
      661 actcttttct ctcgacgggg cttggactat gccgcctgat caaggcgaga cggccgatca
      721 ccgggccgcg ttgcgagtcg cggtgcgatt cgggatggtt aaggcgccaa gccacttcag
      781 gagcgtgcga tgcgtatcga attcgtcccg gccgaccttc atgccggcgc cacggccgcc
      841 gtcgcggtcc tggcccatga ggctgcggcc ctgtcgcccg aagcccgggt gatcgacgag
      901 gccaccggcg gcgccctggc gcgcgccatc gccggcggac gctttaccgg cgccaagggc
      961 cagattctgg atctggtcgc gccgcacggc ctggaagccg cgcgcgtggt gctggtgggc
     1021 gtcgatggcg tcggcgcggt cgagccagag gccgttgagg ccgccgccgc ccatgccttc
     1081 caggccgtga aggcctcggg cgtgaccgaa ctggtcctga agctgggcgc cgacgccgag
     1141 acggctgcgc gcgcagcctt cggcgtcaag ctggccgcct atcgcttcga caagtatcgc
     1201 accaccgaga agaccgaga
//
LOCUS       DAOMEO010001132         1218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11438_length_1218_cov_1.879622, whole genome shotgun sequence.
ACCESSION   DAOMEO010001132 DAOMEO010000000
VERSION     DAOMEO010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_1218_cov_1.879622"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..695)
                     /locus_tag="PLH31_19070"
     CDS             complement(240..695)
                     /locus_tag="PLH31_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91331.1"
                     /translation="MKTISKTLIAGAITAAMVLPASGAFAGEKTNKAILGAVLGGLAG
                     AALGDGDKGAVVIGAVAGAALGASTAKDKDDRRYNSNYRTRPAYNGYSQGYSQGGYQV
                     RDPRYQRDSRYGQGGYGQTGYGQTRYGQAGYGQTGYDARYDTGYANYRR"
     gene            complement(804..>1218)
                     /locus_tag="PLH31_19075"
     CDS             complement(804..>1218)
                     /locus_tag="PLH31_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR91332.1"
                     /translation="RRLASTPTFNSHEWSLLTGVVTGALSLVLIVPAFLAGGAAHGSS
                     AWTLFWGVSLAVALGASVIGNGLWNAASRLLPLSLSGQLIVFETLFALLYGFLHEGRW
                     PRPLESAAIALMLAGVLWSVSLHRPSGVASNMTGT"
BASE COUNT          222 a          374 c          431 g          191 t
ORIGIN      
        1 agatttagca gtgatgatga agcgtaaaga agaagttact tcggggctaa ataaaggccg
       61 actgaaagcg cgatgggtta agaagccgct ccgctctcac ggcgctatag cagatttcgc
      121 agggcgaaca gcggtcgcgg cgaccttgcg taaagattgt ctcgcgtcag gcgcgaaaaa
      181 gcgccgcccc ctgatgaagg gagcggcgca agtcttcgcc gcaggatcgg gatcagggcc
      241 tagcgacggt agttggcgta gccggtgtcg tagcgggcgt cgtagccagt ctggccatag
      301 ccggcctggc cgtagcgggt ctggccatag ccggtctggc catagccccc ctggccgtag
      361 cggctgtcgc gctgatagcg cgggtcgcgg acctggtagc cgccctgcga atagccctgc
      421 gaatagccgt tataggctgg acgggtgcga tagttgctgt tgtagcgacg gtcgtccttg
      481 tccttggcgg tcgaggcgcc gagcgccgcg cccgcaacgg cgccgatgac caccgcgccc
      541 ttgtcgccat cgcccagagc cgcgcccgcc aggccgccga gaacggcgcc caggatggcc
      601 ttgttggtct tctcgccggc gaaggcgccg gaagcgggca ggaccatggc ggcggtgatg
      661 gcgccagcga tcagggtctt ggaaatggtc ttcatggcag tgctcctttc gagaagcgtc
      721 gccaggtccg ggagacgtcc ggtgcggcgc tgttgatacc cgttctaggg ggcgccgctt
      781 gaacccgatc tgaacgttgc ggttcaggtt ccagtcatgt ttgaggctac cccggacggc
      841 cggtgcagcg ataccgacca cagcaccccg gccagcatca gggcgatggc ggcgctctcc
      901 agcgggcgcg gccagcggcc ttcgtgcagg aagccgtaaa gcagggcgaa cagggtttcg
      961 aacacgatca actggccgga aaggctgagc ggcagcaggc ggctggcggc gttccacagg
     1021 ccgttgccga tcaccgaggc gccaagggcc accgccagac tgacgcccca gaacagggtc
     1081 caggcgctgg acccatgcgc cgctccgccg gcgaggaagg ccggaacgat cagaaccagc
     1141 gacagcgcgc cagtgaccac cccggtcagc agcgaccatt catggctgtt gaaggtgggc
     1201 gtcgaggcca gccggcgg
//
LOCUS       DAOMEO010001133         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11445_length_1217_cov_2.595525, whole genome shotgun sequence.
ACCESSION   DAOMEO010001133 DAOMEO010000000
VERSION     DAOMEO010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11445_length_1217_cov_2.595525"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLH31_19080"
     CDS             complement(<1..1145)
                     /locus_tag="PLH31_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HQR91333.1"
                     /translation="MSGPVVGEEGNAGCILAAGGAFAVLFGTAWLLEKVMPTSIASTI
                     AWVALVVFLVLVWKYGFKGRRNALLGSAAFGGIDDVRALASNKGDLLIGRSFRTGKLL
                     RYDGPAHLLTMAPTRSGKGVGTIIPNLLTIDRSVICIDPKGENARVTARTRATKGKVW
                     CLDPFGVSGQPAARYNPLDRLDPESLDLAEDAMTLADALVHDSAGQGGEAHWNEEAKA
                     LIAGLILYGVVHEDRERRTLATVRDYLTLAPKDFADLLARMQDSHGAGGLIARAANRH
                     LGKSDREAAGVLSSAQRHTHFLDSPRIVASTSASDFTFAGLKEGVSTVFLCLPPDRLD
                     AYSRWLRLLIAQALTDMARSAVKPARPVLFLLDEFAALGRLEPVERA"
BASE COUNT          222 a          364 c          437 g          194 t
ORIGIN      
        1 atggcgcgct cgacgggttc gagcctgccc agggcggcga actcatcgag caggaaaagg
       61 acgggccgcg ccggtttgac tgccgagcgg gccatgtcgg tcagtgcctg agcgatcagc
      121 aggcgcagcc agcgcgaata ggcatcgaga cgatcggggg gcagacagag aaacaccgtg
      181 gaaaccccct ctttcaaccc tgcaaaggtg aagtcagagg ccgatgtgct ggcgacgatg
      241 cgggggctgt cgagaaaatg ggtatggcgc tgggccgagg acaacacccc tgcggcttcg
      301 cgatcggact tgccgaggtg gcggttggcg gcgcgggcta tgaggccgcc ggcgccgtgg
      361 ctgtcctgca tgcgggcgag cagatcggca aaatccttgg gtgcgagcgt gagatagtcg
      421 cgcaccgtcg ccagggtgcg gcgctcgcga tcctcgtgga ccacgccata gaggatcagg
      481 cccgcgatca gcgccttggc ttcctcgttc caatgcgcct cgccgccttg ccctgcggaa
      541 tcgtggacca gagcatcggc cagcgtcatg gcgtcttcag ccagatcgag gctttccggg
      601 tctagccggt cgagcggatt atagcgggcg gcgggttgcc cggagacgcc gaagggatcg
      661 aggcaccaga ccttgccctt ggtggcgcgg gtgcgggcgg tgacgcgggc gttctcgccc
      721 ttagggtcga tgcagatcac cgagcggtcg atggtcagca gattgggaat gatggtgcct
      781 acgcccttgc cgctgcgcgt aggggccatg gtcaggagat gggcggggcc gtcatagcgc
      841 agcagcttgc cggttctgaa ggatcgacca atcaggagat cgcccttgtt gctcgcaagg
      901 gcgcgcacgt catcgattcc accgaaggcg gccgagccga gcagcgcgtt gcggcgaccc
      961 ttgaaaccat atttccagac cagcacgagg aagacaacga gcgccaccca ggcgatggtg
     1021 ctggcgatcg aggtcggcat caccttttcg agcagccagg ccgtgccgaa caggacggca
     1081 aaggctccgc cggcagcaag gatacagcct gcgttgccct cttctccgac aaccggccca
     1141 ctcatttttg cgggtccaga gggcgggtga accagatgag gcgcagccaa gggacgggct
     1201 ggccggggcg gccttcc
//
LOCUS       DAOMEO010001134         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11448_length_1217_cov_2.512909, whole genome shotgun sequence.
ACCESSION   DAOMEO010001134 DAOMEO010000000
VERSION     DAOMEO010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11448_length_1217_cov_2.512909"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLH31_19085"
     CDS             <1..819
                     /locus_tag="PLH31_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQR91334.1"
                     /translation="LDKAMEAVKSAPETAILNRADVKLRAPIQPPPQMRDCSCFELHL
                     RQCYAAGREMRAKAKGVEVIADTGPSAAEQRILDLFAKQPIYYKANRFSVIGTEETVI
                     WPSYSRSMDFELEYGCYIKKAAKDVPVEKARDYIFGYTIYNDFSARDAQAVEMIGQLG
                     PAKGKDFDTGNAMGPCIVTADEMPDPYALTMIARVNGEEWGRGSSSTMHWKFEDLIAY
                     ISRSETLYPGEFLGSGTVGNGCGLEAQRFLKPGDEVELDVEGIGVLRNRIVRPA"
     gene            complement(816..>987)
                     /locus_tag="PLH31_19090"
     CDS             complement(816..>987)
                     /locus_tag="PLH31_19090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR91335.1"
                     /translation="IDEAAARAEMVGSIPAGRFGRPSELGDACAFLCSDRAGYITGQN
                     LQLDGGAYGGLI"
     assembly_gap    988..997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<998..1193)
                     /locus_tag="PLH31_19095"
     CDS             complement(<998..1193)
                     /locus_tag="PLH31_19095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HQR91336.1"
                     /translation="MVAITSAMVKTPKFPMALSTGPRAGLTAFCKALAGEVAGDGVTV
                     NCILPEKIETDRLAQMTRSLA"
BASE COUNT          224 a          387 c          404 g          192 t
ORIGIN      
        1 ctcgacaagg cgatggaggc ggtcaagtcc gcgcccgaga cggcgatcct gaaccgcgcc
       61 gacgtcaagc tgagggcgcc gatccagccg ccgccgcaaa tgcgcgattg cagctgcttc
      121 gagctgcacc ttcgccagtg ctatgcagcc gggcgtgaaa tgcgggccaa ggccaagggc
      181 gtcgaggtga tcgccgacac cgggccgtcg gcggccgagc agcgcatcct cgacctcttc
      241 gccaagcagc ctatctacta caaggcaaac cgcttttccg tgatcgggac cgaagagacg
      301 gtgatctggc cgtcctattc gcgcagcatg gatttcgagc tggagtacgg ctgctacatc
      361 aagaaggcgg ccaaggacgt gccggtcgag aaggcccgcg actacatctt cggctatacg
      421 atctacaacg acttcagcgc ccgcgacgcc caggcggtcg agatgatcgg tcaactcggg
      481 cccgccaagg gcaaggattt cgacaccggc aacgcgatgg gaccttgcat cgtcacggcc
      541 gatgagatgc ctgatcccta tgccctgacc atgatcgccc gggtcaatgg cgaggagtgg
      601 ggtcggggct catccagcac catgcactgg aaattcgaag acctgatcgc ctacatctcg
      661 cggtccgaga cgctttatcc cggcgagttc ctgggttcgg ggacggtcgg caacggctgt
      721 ggtctggagg cccagcggtt cctcaagccc ggcgacgagg tcgagctgga cgtcgaaggg
      781 atcggcgtgc tgcgcaaccg gatcgtaagg cccgcctaga tcaggccgcc ataggcgccg
      841 ccgtccagtt gcaggttctg accggtgata tagccggcgc gatccgaaca caggaaagcg
      901 caggcgtcac cgagctccga aggacgtccg aaccggccgg cggggatcga gccgaccatc
      961 tcggcgcggg ccgcggcctc gtcgatcnnn nnnnnnngcg ccagggatcg ggtcatctgc
     1021 gccagtcgat cggtctcgat cttctcgggc aggatgcagt tgaccgtgac gccgtcgccc
     1081 gcgacctcgc cggccagggc cttgcagaag gcggtcagcc ccgcgcgggg tccggttgac
     1141 agggccatcg ggaatttcgg ggtcttgacc atggccgagg tgatggcgac cacgcggccg
     1201 aaacccctct ggcgcat
//
LOCUS       DAOMEO010001135         1217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11449_length_1217_cov_2.391566, whole genome shotgun sequence.
ACCESSION   DAOMEO010001135 DAOMEO010000000
VERSION     DAOMEO010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11449_length_1217_cov_2.391566"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="mnmE"
                     /locus_tag="PLH31_19100"
     CDS             complement(<1..925)
                     /gene="mnmE"
                     /locus_tag="PLH31_19100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQR91337.1"
                     /translation="MAQTIFALATAAGRAALAVVRVSGPATRNVVAALAGKVPPPRQA
                     ALRRLKHDGVALDEALVLFFQGPGSYTGEDAAEFHVHGGRAVVEALLDALAALGLRLA
                     EPGEFTRRAFENGKLDLAQAEGVADLIDAETEGQRKQALGQIGGALSQRYDRWRDLLV
                     HALAMLEAAVDFPDEDLPEEVAARARPGLETLAAEIDAALSDVSRGRRIRDGYRIALV
                     GAPNAGKSTVLNGLAERDAAIVTEIAGTTRDIIEVPLVLGGYRVLLADTAGLRETTDV
                     VEAEGVRRARAWAHDADLRIWVVDGFHVEHLA"
     gene            complement(925..1170)
                     /locus_tag="PLH31_19105"
     CDS             complement(925..1170)
                     /locus_tag="PLH31_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HQR91338.1"
                     /translation="MKMTIEIDCTPVEARTFLGLPDVTALNDHMVKEMQTRMDANMAM
                     LAPEELMKNWMAFGTGAQDQFRKLMTAAAGAAVGKRD"
BASE COUNT          184 a          434 c          403 g          196 t
ORIGIN      
        1 ccgccagatg ttccacgtga aaaccatcaa cgacccagat ccgcaggtcg gcatcgtgcg
       61 cccaggctcg ggcgcggcga acgccttccg cttccacaac atctgttgtc tcgcgcaggc
      121 cggccgtgtc ggccagcagg acgcgatagc cgcccagcac cagcggaact tcgatgatgt
      181 cgcgcgtggt gcccgcaatc tccgtgacaa tggccgcatc gcgctcggcc agcccattga
      241 gcaccgtgct tttaccggcg ttcggcgcac cgaccagggc gatgcgataa ccgtcgcgga
      301 tacggcgtcc acgggaaaca tcggacaagg cggcgtcgat ctcggccgcc agcgtctcca
      361 gtccaggtcg ggcgcgcgcc gcgacctctt ccggcagatc ctcgtctgga aagtcgaccg
      421 cggcttccag catggcgagg gcatggacca gcaggtcccg ccagcggtcg tagcgctggc
      481 tcagtgcgcc gccgatctgg ccgagcgcct gtttgcgctg accctccgtc tcagcgtcga
      541 tcaggtcggc cacgccctcg gcctgcgcga ggtcgagctt gccgttctcg aacgcgcggc
      601 gggtgaattc gccgggctcg gccagacgga ggcccagggc cgccagggcg tccagcagcg
      661 cctcaacgac ggcccgcccc ccgtgcacgt gaaactcggc ggcgtcctcg ccggtgtagc
      721 tgccgggtcc ctggaagaag aggacgagcg cttcgtccag cgcgacgccg tcatgcttca
      781 ggcgtcgaag cgcggcctga cgcggcggcg ggaccttgcc cgccagcgcc gccacaacgt
      841 ttcgcgtggc ggggccggat acgcgcacca cggccaaagc cgcccgtccg gccgccgtgg
      901 ccagggcgaa gatcgtctgc gccatcagtc gcgcttgccg accgccgcgc ccgccgccgc
      961 tgtcatcagc ttgcgaaact ggtcctgggc gccggtcccg aaggccatcc agttcttcat
     1021 cagctcctcg ggcgccagca tggccatgtt ggcgtccatg cgcgtctgca tctccttgac
     1081 catgtggtcg ttcagggcgg tgacatcggg gaggcccaga aaggtgcgcg cctccacggg
     1141 ggtgcagtcg atctcgatgg tcatcttcat ggcggttccc tcaggtcagg gcacgatatt
     1201 gttatgggcc aggcccg
//
LOCUS       DAOMEO010001136         1214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11481_length_1214_cov_2.509060, whole genome shotgun sequence.
ACCESSION   DAOMEO010001136 DAOMEO010000000
VERSION     DAOMEO010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11481_length_1214_cov_2.509060"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLH31_19110"
     CDS             <1..274
                     /locus_tag="PLH31_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HQR91339.1"
                     /translation="GVSVSVLMREALGLVDARRRRPMPKADPALLRELGRIGGNLNQV
                     ARWLNSASATGSHPDIDALVVATKLVAIERALTMLANPPGPDQPPC"
     gene            392..>989
                     /locus_tag="PLH31_19115"
     CDS             392..>989
                     /locus_tag="PLH31_19115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HQR91340.1"
                     /translation="MVVRDPLPEVMRGNAALIEAEIDACPHQWTYRSGVLSFTREDAP
                     TERQQGEVMDAFERLAFAGLERDQWDILWVRHTHEDRVELHFVTPRMEFSSGRSLNIA
                     PPGYQKAFDALRDVENKRHGWADPEDPARARDVVSLVELARRGQAREVIHDWLLDRIT
                     EGKVQDRSSMVAALNEAGFQIPRAGKDYITALDADTNER"
     assembly_gap    990..999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1029..>1214
                     /locus_tag="PLH31_19120"
     CDS             1029..>1214
                     /locus_tag="PLH31_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91341.1"
                     /translation="MSERLSAQAIEPATANQTSEAKADDLPDSTLTNFKAACERLSSS
                     ALSTIEADMANLVLANRR"
BASE COUNT          224 a          403 c          398 g          179 t
ORIGIN      
        1 cggcgtgagc gtatccgtcc tgatgcgcga ggccttgggc ctagtggacg cgcgccggcg
       61 ccgtcccatg cccaaggctg atcccgcgtt gctgcgcgaa ctcgggcgca tcggcggaaa
      121 tctcaatcag gttgcccgct ggctgaatag cgccagcgca accggctcgc acccggacat
      181 cgacgcgctc gtcgtggcga ccaagcttgt cgccatcgag cgcgccctga cgatgctcgc
      241 aaacccgccc ggaccggatc agccgccatg ctgatcaagt tcttcaaaaa cggggcgtgg
      301 cgcgggaccg gtcggctatc tgatcgcccg cgaagtcgtc gcctacagcg agaaccgcga
      361 cgcgctgcgt gacgcatcag gccggacggt gatggtggtc cgtgaccctc tgcctgaagt
      421 gatgcgcggc aacgcggcgc tcatcgaagc cgagatcgac gcatgtcctc atcagtggac
      481 ctaccgctcg ggcgtcctgt ccttcacgcg ggaggatgcg ccgaccgagc gccagcaggg
      541 cgaggtgatg gacgccttcg agcgactggc gttcgccggc ctggagcgtg accaatggga
      601 tatcctctgg gtgcgacaca cccacgagga ccgggtcgag ctccacttcg tgactccccg
      661 gatggagttc tccagcgggc gcagcctgaa catcgcgccc ccgggctacc aaaaggcgtt
      721 cgatgcgctt cgcgatgtcg agaacaagcg ccacggctgg gccgatccag aggacccggc
      781 gcgggctcgc gatgtcgtca gtctggtcga actggcgcgg cgcggccagg cccgagaggt
      841 catccacgac tggcttctcg accgcatcac cgagggaaag gtccaggacc gttcgagcat
      901 ggtcgccgcc cttaacgagg ccggctttca gatcccacgc gccggcaagg actacatcac
      961 cgcgctcgac gccgacacca acgagcgccn nnnnnnnnnc cgtgaagatt ggaccagagc
     1021 ggcagccgtt gagcgagcgc ttgagcgctc aggcgatcga gccggcgacg gcgaaccaaa
     1081 ccagcgaagc gaaggccgac gacttgccgg actcgacgct gacgaacttc aaagccgctt
     1141 gcgagcgttt gagcagcagc gcgctgagta caatcgaggc cgatatggcc aacctcgtgc
     1201 tcgcgaaccg gagg
//
LOCUS       DAOMEO010001137         1211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11523_length_1211_cov_2.562284, whole genome shotgun sequence.
ACCESSION   DAOMEO010001137 DAOMEO010000000
VERSION     DAOMEO010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_1211_cov_2.562284"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLH31_19125"
     CDS             complement(<1..341)
                     /locus_tag="PLH31_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HQR91342.1"
                     /translation="MKFATALLLVAIMGGDALAGSLTVTVRGANGKPVSDAVVTVHPG
                     GQPSRGPIKFPWPYRVQQQNIQFSPFVLIVPAGAEVAFPNLDKVRHHVYSFSPGNKFE
                     LKLYGREENRIV"
     assembly_gap    354..417
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            428..>1211
                     /locus_tag="PLH31_19130"
     CDS             428..>1211
                     /locus_tag="PLH31_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HQR91343.1"
                     /translation="MAGTLGCAAGATAAEIETGGKLLLTGGVSSLEGGAGGGLATWAV
                     IAGNETNNGVGGKVHATYVDVSDYTLRAAGAAVGLHDRIELSVARQEFDTGATGAKLG
                     LGKGFTFKQDIVGVKLRVLGDAVYEQDTWVPQVSIGAQYKKNNQDAIVRAIGGRKDSG
                     VDYYVAATKLVLDKSLVLSGAMRLTKANQTGLLGFGGDKNDSYTAQFEGSAGLLLSKR
                     LAVGAEYRTKPSNLGFAREDDWVDLFAAYAINKTVSVTAAYVD"
BASE COUNT          211 a          358 c          396 g          182 t
ORIGIN      
        1 acgatacggt tctcttcccg cccgtacagc ttcagctcga acttgttgcc cggcgaaaag
       61 gaatagacgt ggtggcgcac cttatcgagg ttggggaacg ccacctcggc cccggcgggc
      121 acgatcagca cgaagggcga gaactggatg ttctgctgct gaacgcggta aggccagggg
      181 aacttgatcg gcccccggct gggctggccg ccgggatgaa ccgtgaccac ggcgtctgaa
      241 accggctttc cattggcgcc gcgcaccgtg acggtcaggc tgcccgccag ggcgtcgccg
      301 cccattattg caaccaggag aagtgcggta gcgaatttca tgccgaacat ctannnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc
      421 ggcggtcgtg gccggaaccc tgggatgcgc cgccggcgcg accgcggccg agatcgaaac
      481 cggcggcaag ctgttgctga ccggcggcgt cagttcgctg gaaggcggcg cgggcggcgg
      541 cctggccacc tgggcggtca tcgcgggtaa cgagaccaac aacggcgtcg gcggcaaggt
      601 ccatgcgacc tatgtcgacg tctccgatta caccctgcgc gccgcgggcg cggctgtcgg
      661 cctccatgac cgcatcgagc tgtcggtcgc ccgccaggag ttcgataccg gcgccacggg
      721 cgccaagctg ggcctgggca agggcttcac cttcaagcag gacatcgtgg gcgtgaagct
      781 gcgggtgctg ggcgatgcgg tgtatgagca ggacacctgg gtcccgcagg tgtcgatcgg
      841 cgcgcagtac aagaagaaca atcaggacgc cattgtccgc gccatcggcg gcaggaagga
      901 cagcggcgtc gactactatg tcgccgccac caagctggtg ctcgacaaga gcctcgtctt
      961 gagcggcgcg atgcgcctga ccaaggccaa ccagaccggc ctgctgggct tcggcggcga
     1021 caagaacgat agctacacgg cccagttcga aggttcggcc gggttgctgc tcagcaaacg
     1081 cctggcggtg ggcgccgaat atcgcaccaa gcccagtaat ctgggcttcg ccagggaaga
     1141 cgactgggtc gacctgtttg ccgcctatgc gatcaacaag acggtgtcgg tgacggccgc
     1201 ctatgtcgat c
//
LOCUS       DAOMEO010001138         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11585_length_1207_cov_2.157986, whole genome shotgun sequence.
ACCESSION   DAOMEO010001138 DAOMEO010000000
VERSION     DAOMEO010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11585_length_1207_cov_2.157986"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLH31_19135"
     CDS             <1..713
                     /locus_tag="PLH31_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQR91344.1"
                     /translation="FFTGHWDHLGVGQPDAKGDTIYNGAVDNATGIAAMLEMGRVFAK
                     GPRPDRSLVFLAVTAEEKGLLGSEYYAANPLYPLAKTVGVLNTDALDPAGPARDFTIS
                     GNAKLGLLDELIAHGKKVGRTYTPDPATEAGYFYRSDHFPFAKRGVPAISFGSGEDLV
                     EGGVAAGKAFADAYRVNAYHQPADEWNASWRLDGMTADISLIQGLGADLANSTRWPEW
                     GEGSEFKGTRDATKAERK"
     gene            complement(724..852)
                     /locus_tag="PLH31_19140"
                     /pseudo
     CDS             complement(724..852)
                     /locus_tag="PLH31_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            890..>1207
                     /locus_tag="PLH31_19145"
     CDS             890..>1207
                     /locus_tag="PLH31_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HQR91345.1"
                     /translation="MTASVFDLFKLGVGPSSSHTMGPMTAAGLFVGRLREAGTLGAVA
                     RVETRLYASLALTGRGHATDRAVILGLMGFVPADLDPDAGEAALVETRANQWLTLGGE
                     TGMA"
BASE COUNT          194 a          430 c          398 g          185 t
ORIGIN      
        1 tgttcttcac cggccactgg gaccatctgg gcgtgggcca gcccgacgcc aagggcgaca
       61 cgatctataa cggcgccgtc gacaacgcga ccggcatcgc cgccatgctg gaaatgggcc
      121 gggtgttcgc caagggtcca cgccctgacc gctcgctggt cttcctggcc gtgaccgccg
      181 aggaaaaggg cctgctgggc tcggaatatt acgcggccaa tccgctgtat ccgctggcca
      241 agaccgtggg cgtgctcaac accgacgccc tcgaccccgc cggtccggcc agggacttca
      301 ccatctcggg caacgccaag ctcggtttgc tggacgagtt gatcgcccat gggaaaaagg
      361 tgggccgcac ctatacgccc gaccctgcca ccgaggccgg ctacttctat cgctcggacc
      421 atttcccctt cgccaagcgt ggcgtgccgg ccatctcgtt cggctcgggc gaggatctgg
      481 tcgagggcgg cgtcgcggcc ggcaaggcct tcgccgacgc ctatcgcgtc aacgcctatc
      541 accagcccgc cgacgagtgg aacgccagtt ggcgcctgga cggcatgacc gccgacatca
      601 gcctgatcca gggcctgggc gcggatctgg ccaactccac ccgctggccc gaatggggcg
      661 agggttcgga gttcaagggt acgcgcgacg cgaccaaggc tgaacggaag tagaccgcac
      721 ctctaacttg tcatcccgga agcctcgcag aggctgtccg ggacccaggg gccgacgcgc
      781 tgcggttgcc cctgggtccc ggctctccgc ttcgctgcgg ccgggatgac aaacatattt
      841 tgaaccggac agcgctcacc gccacgcggg agagcgggat gccatcacca tgacagcctc
      901 ggtcttcgac ctcttcaagc tcggcgtcgg tccgtcgagc agccacacca tgggcccgat
      961 gacggccgcc gggctgttcg tcggccgcct gcgtgaggcc ggaacgctgg gcgcggtcgc
     1021 gcgggtcgaa acccgcctct acgcctccct ggccctgacc ggacggggcc acgccaccga
     1081 ccgcgccgta attctgggcc tgatgggctt cgtccccgcc gacctcgatc ccgacgccgg
     1141 cgaggcggcg ctggtcgaga cgcgggccaa ccagtggctg accctgggcg gcgagacggg
     1201 gatggcc
//
LOCUS       DAOMEO010001139         1205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11614_length_1205_cov_2.638261, whole genome shotgun sequence.
ACCESSION   DAOMEO010001139 DAOMEO010000000
VERSION     DAOMEO010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11614_length_1205_cov_2.638261"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLH31_19150"
     CDS             <1..272
                     /locus_tag="PLH31_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HQR91346.1"
                     /translation="PTVERDGMLLMVDDDRFPLRPGEVPVERSVRFRTLGCYPLTGAV
                     ESTASTLSEIIQETLLTTTSERQGRAIDKDAGGAGMEKKKQEGYF"
     gene            272..518
                     /locus_tag="PLH31_19155"
                     /pseudo
     CDS             272..518
                     /locus_tag="PLH31_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676564.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <529..>1205
                     /locus_tag="PLH31_19160"
     CDS             <529..>1205
                     /locus_tag="PLH31_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQR91347.1"
                     /translation="VGYDQARYDAIVADYAAFALSLGITAFTPMPISGFKGDNITEPS
                     DNTPWYSGPTLMQHLETVEVGIATDQAKPLRMAVQWVNRPNLDFRGFSGQISSGSVKP
                     GDAIRVLPGGKTSTISRITTLGADLDEAVAGQSVTLTFADEIDCSRGSVIAAADAPPE
                     VSDQFEATIVWMDDDPLIVGRSYWLKLGTQLVSATVHQPKYQVNVNTMEHLAAKTLEL
                     NAIGVAEL"
BASE COUNT          223 a          400 c          385 g          187 t
ORIGIN      
        1 gcccgaccgt ggagcgcgac ggcatgctgc tgatggtcga tgacgaccgc ttcccgctgc
       61 gccccggcga agtccccgtc gagcgctcgg tccgcttccg cacgctgggc tgctacccgc
      121 tgaccggggc ggtcgagagc acggcctcga ccctgtcgga gatcatccag gagaccttgt
      181 tgaccacgac ctccgagcga cagggccgcg cgatcgacaa ggatgcgggc ggcgccggca
      241 tggaaaagaa gaagcaggaa gggtatttct gatgacgacc ggcccttccg gcgcgcggga
      301 cagcgtctat cagaccgacg cgctgatcgc cgaagacatc gacgcctatc tcgaggcgca
      361 ccagcacaag acgatgctgc ggttcatcac ctgcggcagt gttgatgacg gcaagtccac
      421 gctgatcggg cgactgctgt acgacagcaa gatgatcttc gaggaccaac tggcggctct
      481 tgaagccgac tcccggcggg tgggcaccca gggtcaggnn nnnnnnnngt cggctacgat
      541 caggccaggt acgacgccat cgtcgccgac tacgccgcct tcgcgctgag cctcgggatc
      601 accgccttca cgcccatgcc aatttccggc ttcaagggcg acaacatcac cgagcccagc
      661 gacaacacgc cctggtatag cggcccgacg ctgatgcagc accttgagac cgtcgaggtg
      721 ggcatcgcga ccgatcaggc caagccgctg cgcatggccg tgcagtgggt gaaccggccc
      781 aatctggatt tccgcggctt ctccggtcag atcagtagcg gctcggtcaa gccgggcgac
      841 gcgatccgcg tgctgccggg cggcaagacc tcgaccatca gccgcatcac caccctgggc
      901 gccgatctgg acgaggcggt ggcgggccag tcggtcacgc tgaccttcgc cgacgagatc
      961 gactgctcgc gcggcagcgt catcgcagcc gccgacgctc cgcccgaagt gtcggaccag
     1021 ttcgaggcga ccatcgtctg gatggatgac gatccgttga ttgtcgggcg gtcctactgg
     1081 ttgaagttgg gaacccaact cgtctccgcc accgttcatc agcccaagta ccaggtgaac
     1141 gtcaacacga tggagcacct ggccgccaag acgctggagc tgaacgccat cggcgtggcc
     1201 gagct
//
LOCUS       DAOMEO010001140         1204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11628_length_1204_cov_2.901654, whole genome shotgun sequence.
ACCESSION   DAOMEO010001140 DAOMEO010000000
VERSION     DAOMEO010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11628_length_1204_cov_2.901654"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1204)
                     /locus_tag="PLH31_19165"
     CDS             complement(<1..>1204)
                     /locus_tag="PLH31_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HQR91348.1"
                     /translation="VARVRAETGLTIRVLTGEEEARYAALGVLAGAPSATGVVGDLGG
                     ASLELIRLEPTGAGIGVTLPLGPFSLGLSEGFDGERVRRLCAQRLAPVAAAFRTDSFH
                     AVGGAWRNLALLHMRLSGYPLHVVHQYEISASEALDAARLVSHQSRNSLERIEGMSKK
                     RSETLPYAAVVLESLIDSLALKRIEISAFGVREGLLFEAMPPRVRGLDPLIEGCASLG
                     ARQGVADELGEGLNAWIAPAFISLPPLFDDGRDAVLTSAACRLADLGARLHPDHRADL
                     VFEQVLRAPIAGQRHPERAFLAAAAYARHSTAFNPPELEVLEHLLTPARLKRARALGA
                     AIRLGCDLSGRSAPLLSRSRLSIDRTQLTLTAEPGYADLLLGEQTSKRAGAVAQILQR
                     GELPVQVQE"
BASE COUNT          172 a          417 c          459 g          156 t
ORIGIN      
        1 ttcctgtacc tggacgggca gttcaccgcg ctgcaggatc tgggcgaccg ctccggctcg
       61 cttgctggtc tgctcgccca gcagcaggtc tgcatagccc ggctcggcgg tcagggtcag
      121 ctgggtcctg tcgatcgaga ggcgtgagcg cgacagcagg ggcgcactgc ggcccgagag
      181 atcgcagccc agccggatgg ccgcgcccag ggcccgggcg cgcttcagac gggccggggt
      241 gagaaggtgc tcaagcacct ccagttcggg cgggttgaac gcggtggaat ggcgggcgta
      301 ggcggcggcg gccaggaagg cgcgttccgg gtggcgctgg ccggcaatgg gcgcgcgcag
      361 cacctgttcg aacaccaggt cggcgcgatg gtcggggtgc aggcgagcgc ccagatcggc
      421 caggcggcag gcggccgagg tcaacaccgc gtcgcgtccg tcatcgaaca gcggcggcag
      481 gctgatgaac gccggggcga tccaggcgtt caggccttcg cccagctcgt cggcgacgcc
      541 ctgccgggcg cccagcgagg cgcagccctc gatcagcggg tccaggcccc gcacccgggg
      601 cggcatggcc tcgaacagca ggccctcgcg cacgccaaag gccgagatct cgatgcgctt
      661 gagcgccagg ctgtcgatca ggctttccag caccacggcg gcgtagggca gggtctcgga
      721 gcgcttcttg ctcatgccct cgatgcgttc cagcgagttt cgggactggt gcgagaccag
      781 gcgcgcggcg tccagcgcct cgctggcgct gatctcgtac tggtggacca cgtgtagcgg
      841 atagcccgac agccgcatgt gcagcagggc caggttgcgc caggcgccgc ccacggcgtg
      901 gaagctgtcg gtcctgaagg cggcggccac gggggccagg cgctgggcgc acaggcggcg
      961 cacgcgctcg ccgtcgaagc cttccgacag gcccaggctg aacggaccca ggggcagggt
     1021 gacgccgata cccgcgccgg tgggctccag gcgaatcagc tccaggctgg cgccgcccag
     1081 gtcgcccacc acgccggtgg ccgacggcgc gccggccagg acgcccagcg cggcgtagcg
     1141 cgcctcttcc tcgccggtca ggacgcggat ggtcaggccc gtctcggccc tgacccgggc
     1201 cacg
//
LOCUS       DAOMEO010001141         1204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11632_length_1204_cov_2.543081, whole genome shotgun sequence.
ACCESSION   DAOMEO010001141 DAOMEO010000000
VERSION     DAOMEO010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11632_length_1204_cov_2.543081"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLH31_19170"
     CDS             <1..237
                     /locus_tag="PLH31_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQR91349.1"
                     /translation="IGFEDVAINEDVSKVSVIGVGMRSHAGVAQSMFQALAEKNINIQ
                     VISTSEIKISVLIDAAYTELAVRALHAAYGLDQL"
     gene            292..756
                     /locus_tag="PLH31_19175"
     CDS             292..756
                     /locus_tag="PLH31_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91350.1"
                     /translation="MSDVFKFDPAAQTVTFQGEAGLELLYDLLLRAKFGDGYEKPLLV
                     SPWLAALLKQLDKALPDDGQWFPERPGQPIFDTDDLLAMGDAVIEEGHTVGWWSMTEP
                     ERRDYLRETIAAPHPLTDLQVEFIEADIDAALEQARRLVQDAGEPLAMPGHG"
     gene            787..>1204
                     /locus_tag="PLH31_19180"
     CDS             787..>1204
                     /locus_tag="PLH31_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR91351.1"
                     /translation="MTDLRRLTLGLLYGLIAGAFWGGVFLAPLLLVDFTPLQMTAGRY
                     LAYGLASLVLLAPTALAVFGRMTGRDWRDLALLSLLGNLIYYVGLAVAVQNAGVAAAS
                     LIIGLLPVTVTLVGARPGEGVPLRRLAAPLALLAAGV"
BASE COUNT          168 a          410 c          419 g          207 t
ORIGIN      
        1 atcggttttg aggacgtggc catcaacgag gacgtctcga aggtctcggt gatcggggtc
       61 ggcatgcggt cgcacgccgg cgtcgcccag tcgatgttcc aggccctggc cgagaagaac
      121 atcaacatcc aggtgatctc gacctcggag atcaagatca gcgtcctgat cgacgcggcc
      181 tataccgagc tggccgttcg cgccctgcac gcggcgtacg ggctggatca gctgtaggcc
      241 aaacgtcgcc gagtcgcgtt atcaggcgat agcccgacca agagatcccc catgtccgac
      301 gtcttcaagt tcgaccccgc cgcccagacc gtcaccttcc agggcgaggc gggcctggag
      361 ctgctgtacg acctgctgct gcgcgccaag ttcggcgacg gctacgagaa gccgctgctg
      421 gtcagcccgt ggctggcggc gctgctcaag caactggaca aggccctgcc cgacgacggc
      481 cagtggttcc ccgagcgtcc gggtcagccg atcttcgaca ccgacgacct gctggccatg
      541 ggcgacgcgg tgatcgagga aggccacacc gtcggctggt ggtcgatgac cgagccggag
      601 cgccgcgact atctgcgcga gaccatcgcc gcgccccatc cgctgaccga cctgcaggtc
      661 gagttcatcg aggccgatat cgacgcggcc ctcgaacagg cccgccgtct ggtccaggac
      721 gccggcgagc cgctggccat gccggggcac ggctagttcc cctatattcg gggcatggcc
      781 cacgatctga ccgaccttcg ccgcctcacg ctcggcctgc tgtatggcct gatcgccggc
      841 gccttctggg gcggggtgtt tctggcgccg ttgctgctgg tcgacttcac gccgctgcag
      901 atgaccgctg ggcggtatct ggcctatggc ctggcgtcgc tcgtgttgct ggcgcccacc
      961 gccctggcgg tgttcggccg gatgacgggc cgcgactggc gggacctggc gctgctgagc
     1021 ctgctgggca acctgatcta ttatgtcggc ctggccgtcg cggtgcagaa cgccggggtg
     1081 gcggcggcct cgctgatcat cgggctgttg ccggtgacgg tgaccctcgt gggcgcgagg
     1141 cccggcgagg gcgtgccgct ccggcggctg gccgccccgc tggccctgct ggccgccggg
     1201 gtgg
//
LOCUS       DAOMEO010001142         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11651_length_1203_cov_1.799652, whole genome shotgun sequence.
ACCESSION   DAOMEO010001142 DAOMEO010000000
VERSION     DAOMEO010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11651_length_1203_cov_1.799652"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLH31_19185"
     CDS             <1..333
                     /locus_tag="PLH31_19185"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQR91352.1"
                     /translation="LAVLRLLLGLTGNLDLGLHFPAAQTLAGLTAMTSNFLINNSLTY
                     RDRRLRGLDLAKGYVRFCVFCGLGLVANVAVATMMTEHGATWWMAGVGGAVIGAAWNY
                     LTTSIAVW"
     gene            327..>1203
                     /locus_tag="PLH31_19190"
     CDS             327..>1203
                     /locus_tag="PLH31_19190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR91353.1"
                     /translation="MVKWLRALRRPELLLLAITVWRLWAAAAIPLTEDEAYYRLWSQH
                     LALGYFDHPPMIAWWIAAGTRILGDTALGVRLLAVLACSATSLLLAQIVRCLGGSQQA
                     ASRAMIWYQATLLVGLGGLLATPDAPTTLLWAVVVWLALKAEVGSPRLWLAAGLAAGL
                     ALLSKYSSLFLPVGIALWLIVRPEGRRILRTPWPWIAGLLALTLFGLNIAWNATHGWL
                     SFSKQFGRAAVHGFSPKHLVELIVIQALLLNPLIAGFAAKGLARLWRTRQAPLLGLLA
                     PTLIAAPFAAYLVLHS"
BASE COUNT          176 a          393 c          365 g          269 t
ORIGIN      
        1 ctggccgtgc tgcgtcttct actgggtttg accgggaacc tcgatcttgg cctgcacttc
       61 ccagcggctc agaccctggc ggggctcaca gccatgacct ccaacttcct catcaacaac
      121 agccttacct accgagatcg gcgcctgcgc gggttggatt tggcgaaggg ctacgttcgg
      181 ttctgcgtgt tctgtggcct gggcctggta gccaatgtcg ctgtcgccac gatgatgacc
      241 gagcacggag ccacctggtg gatggcaggc gtcggcggcg ctgtgatcgg cgctgcctgg
      301 aactatctga ccacttcgat cgccgtatgg tgaagtggct gcgcgcattg cgtcgacccg
      361 aactcctgct gctggcgatc accgtttggc ggctctgggc ggccgccgcg atccccctga
      421 ccgaagacga ggcttactac cgactttggt cgcaacacct cgcccttggc tatttcgacc
      481 atccgccgat gatcgcgtgg tggatagccg cggggactcg aatccttggc gacaccgctc
      541 tgggcgtgcg cctcctggca gtcttagcct gcagcgctac aagcctactc ctggcgcaga
      601 tcgtcaggtg tctgggcggg agccagcaag ccgccagccg tgcgatgatc tggtaccagg
      661 cgacgctcct tgtcggactg ggtggcctcc tggcgacgcc ggacgcaccg acgaccctgt
      721 tgtgggccgt cgtcgtttgg ctcgccctga aggcagaggt cgggagcccc cgtctttggc
      781 tcgccgcagg gctggctgct gggctcgccc tattgtcaaa atattcgtcc cttttcctac
      841 ccgtcggcat cgctctctgg ctgatcgttc gtccggaagg gcggcgaata ttacgaaccc
      901 catggccctg gatcgctggc ctgctggctc taacgctctt tggcttgaac atcgcttgga
      961 atgcaacgca tggctggctg agcttcagca agcagtttgg ccgggccgcc gtacatggtt
     1021 tttcgcccaa gcatctggtc gaactgatcg tgatccaggc gcttctgctc aaccccttga
     1081 ttgcgggctt cgcggccaag ggactggcgc gcctttggcg cacccgccaa gcgcctttac
     1141 tgggcttatt ggctccaacc ctaatcgccg cgccctttgc tgcctacctc gtcttgcaca
     1201 gtc
//
LOCUS       DAOMEO010001143         1198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11714_length_1198_cov_2.228346, whole genome shotgun sequence.
ACCESSION   DAOMEO010001143 DAOMEO010000000
VERSION     DAOMEO010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_1198_cov_2.228346"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..548
                     /locus_tag="PLH31_19195"
     CDS             159..548
                     /locus_tag="PLH31_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR91354.1"
                     /translation="MIEPGDFDMIRFEASAGRAAKLLKALANERRLRLLCQLVDGERS
                     VGQLRPAAGLSQSALSQHLAVLREEGVVATRREGLTVWYRIENPAALQVVAALAEVFC
                     PSPQPENGQLENGRLENVLLETVQGET"
     gene            548..>1198
                     /locus_tag="PLH31_19200"
     CDS             548..>1198
                     /locus_tag="PLH31_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR91355.1"
                     /translation="MDLGALIAAGLGGALIGLLLAVFGGGGSVLATPLLLYVVGVRDP
                     HVAIGTSAAAVSVNALVGLILQARTGAVKWPCALTFAGAGLAGALLGAELAKRLDGGL
                     LLLGFSAVMAAIAVSMLLPRKDAGNPDVRLTPTMTLKLAPMGLGTGLAAGFFGIGGGF
                     LIAPGLMAATGMTLANAAASSLASVALFGAATSLSYAAAGLVNGPLFLALIAGGAVG"
BASE COUNT          167 a          393 c          432 g          206 t
ORIGIN      
        1 cgccgcccgg gtgtcggggt cgacgagcag atagctgacg gtgttggtcg ccgtatcgaa
       61 gaaggcctgc accgacaggg gctcaagagc gagaggagct tgggtcatgg ccatatatta
      121 gacgttgcta aattagattc aagtgatata tagaccccat gatcgagcca ggcgatttcg
      181 acatgatccg gttcgaggcc agcgccggac gcgccgccaa gctgctcaag gccctggcca
      241 acgagcgccg gctgaggctg ctgtgccagc tggtcgatgg cgagcggtcg gtgggacagt
      301 tgcggcccgc cgccggcctg tcgcaatcgg cgctctcgca acacctggcg gtgctgcggg
      361 aggaaggcgt cgtggccacg cgccgcgaag gcctgacggt ctggtacagg atcgagaacc
      421 cggcggccct tcaggtggtg gcggccctgg ccgaggtctt ctgcccctca ccccaacctg
      481 aaaatggcca acttgaaaat ggccgcctcg aaaacgtact tctcgaaacc gtacagggag
      541 agacctgatg gaccttggcg ccctgatcgc ggccggcctt ggcggcgcgc tgataggcct
      601 tctgctggcg gtgttcggcg gcggcggctc ggtgctggcc acgcctctgc tgctctatgt
      661 cgtcggggtg cgagatccgc atgtcgcgat cggcacctcg gcggcggccg tcagcgtcaa
      721 cgccctggtc ggcctgatcc tgcaggcccg caccggcgcg gtgaaatggc cctgcgccct
      781 gaccttcgcg ggcgcgggcc tggccggggc cctgctgggc gccgagctgg ccaagcgcct
      841 ggacggcggc ctgctgctgc tgggcttttc ggcggtgatg gccgccatcg cggtctccat
      901 gctcctgccg cgcaaggacg ccggcaaccc ggacgtgcgc ctgaccccga ccatgaccct
      961 gaaactggcg ccgatgggcc tggggaccgg tctggcggct ggcttcttcg gcattggcgg
     1021 cggcttcctg atcgcgccgg gcctgatggc ggccacgggc atgaccctgg ccaacgcggc
     1081 ggcctcgtcc ctggcctcgg tggcgttgtt tggggccgcg acgagcctca gctacgccgc
     1141 cgccggtctg gtgaacgggc cgctctttct ggccctgatc gcgggcggcg cggtcggg
//
LOCUS       DAOMEO010001144         1197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11723_length_1197_cov_2.378284, whole genome shotgun sequence.
ACCESSION   DAOMEO010001144 DAOMEO010000000
VERSION     DAOMEO010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11723_length_1197_cov_2.378284"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLH31_19205"
     CDS             complement(<1..369)
                     /locus_tag="PLH31_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR91356.1"
                     /translation="MSSVALLVAPLAAHSGEPASIMTAERQKIVSQTLPFSDRQDFDF
                     AERGFLGTRADPLIKRADGQVAWNLAAYDFLKGEAPATVNPSLWRQAQLLARHGLYQV
                     SDSVWQVRGFDLANITFIKGD"
     assembly_gap    411..420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            531..1154
                     /locus_tag="PLH31_19210"
     CDS             531..1154
                     /locus_tag="PLH31_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR91357.1"
                     /translation="MDRDARAQAVLDAAVMVFAEKGLNAATMQDIADRVCMAKILIYR
                     LYPSRQALIDALFSDILTAIETIAAEPWGGYGSGMAALIELGRARPALFLFLLREARG
                     GPETLPWATACDALLSGLTEPFVAAPPEASDDLQAACTHAARTFLPFVIQTWIAGIER
                     NDGMTDAARIKWFGDMVRAWRMATREALGLPDAPEPAVLASRPPRGS"
BASE COUNT          200 a          411 c          369 g          207 t
ORIGIN      
        1 gtcccccttg atgaacgtga tgttggccag gtcaaaaccc ctcacctgcc agacgctgtc
       61 agacacctga tagaggccgt gccgggccag cagttgggcc tgtcgccaga gactggggtt
      121 caccgtggcg ggcgcctcgc ccttcaggaa gtcataggcc gccagattcc aggccacctg
      181 cccgtcggcg cgcttgatca gcgggtcggc gcgggtgccc aggaagccgc gctcggcgaa
      241 gtcgaagtcc tggcgatcgg agaacggcaa cgtctggctg acgatcttct ggcgctcggc
      301 agtcatgatc gaggcgggtt cgccactgtg agcggccagc ggcgccacca gaagcgcaac
      361 tgatgacacg acaagcgccg ctttctggtt aagtttgaat ttgaacaccg nnnnnnnnnn
      421 ttctcgtttg aaactttata gtttcattct acatatcacc gaagatgatt gcagcaaacc
      481 aagctcgagc gcccacgccc cgcggccgtc ctggtcagcc caagccgcgc gtggatcgcg
      541 acgcgcgcgc ccaggctgtg cttgacgccg ccgtgatggt cttcgccgaa aagggcctta
      601 acgccgcgac gatgcaggac atcgccgacc gggtctgcat ggccaagatc ctcatctatc
      661 ggctctatcc gtctcgccag gccctgatcg acgccctctt cagcgatatt ctgacggcga
      721 tcgaaaccat cgccgccgag ccctggggcg gctacgggtc aggcatggcg gcgttgatag
      781 aactggggcg cgcccggccc gcactcttcc tctttcttct ccgcgaggcg cgaggcggcc
      841 ctgagacctt gccgtgggcg acggcttgcg acgccctgct ctcgggcctg accgagccct
      901 tcgtggccgc tccccccgaa gcgtccgacg acctgcaggc cgcctgcacg cacgccgcgc
      961 gcaccttttt gcccttcgta atccagacct ggatcgcggg catcgagcgc aacgacggca
     1021 tgaccgacgc cgcccgcatc aagtggttcg gcgacatggt ccgcgcctgg cgcatggcga
     1081 cgcgtgaagc cctgggcctg cctgacgcgc ccgagcccgc cgtcctggct agtcggccgc
     1141 ctcgcggttc ttgagccagg cgcgagccac aaggccggcg atgatccaga cgacaac
//
LOCUS       DAOMEO010001145         1196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11738_length_1196_cov_3.078002, whole genome shotgun sequence.
ACCESSION   DAOMEO010001145 DAOMEO010000000
VERSION     DAOMEO010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11738_length_1196_cov_3.078002"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLH31_19215"
     CDS             <1..1152
                     /locus_tag="PLH31_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91358.1"
                     /translation="AVAERDAAQAALFQSQKLEAIGQLTGGVAHDFNNLLTVIIGALD
                     VVDRNPADEARRNRMIAAAMAAAKRGEKLTQHLLAFARRQPLRPEVCRIDRLIAESED
                     LLRRALGDAYSFHLRLGGGVRNALVDSSQFEAALLNLVVNARDATPAGGEVTIETRPV
                     TLPKPVGELPAGQYLRIAVRDTGSGMDAETAARAVEPFFTTKPPGEGTGLGLSQVYGF
                     ARQSGGSVDIETRPGQGATIAMLLPIAAATDPDASEAAFDHVAGQVPARVLLVEDDPQ
                     VAELVDAMLHDLGHTVVRAGGVDEALDRLNADKTIELVLSDVIMPGGRSGVDLAEHLA
                     TARPGLPVVLCSGYTGGDQGRAQAGAWPFLSKPFSLDSLARVLTEARVR"
BASE COUNT          170 a          441 c          415 g          170 t
ORIGIN      
        1 gccgtggccg agcgcgacgc ggcccaggcg gcgctgttcc agtcgcagaa gctggaggcc
       61 atcggtcagc tgaccggcgg cgtggcccat gacttcaaca accttctgac cgtgatcatc
      121 ggcgccctgg atgtggtcga ccgcaacccc gccgacgagg cccgccgcaa caggatgatc
      181 gctgcggcca tggccgccgc caagcggggc gagaagctga cccagcacct gctggccttc
      241 gcccgccgcc agcccctgcg gcccgaggtg tgccgcatcg accgcctgat cgccgagagc
      301 gaggacctgt tgcgccgcgc cctcggcgac gcttacagct tccacctgcg gctgggcggc
      361 ggcgtgcgca acgccctggt cgattccagc cagttcgagg ccgccctgct gaacctggtg
      421 gtcaatgccc gcgacgccac cccggccggc ggcgaggtga cgatcgagac ccgtcccgtc
      481 accctgccca agccggtcgg cgaactgccg gccgggcaat atctgcggat cgccgtccgg
      541 gacaccgggt ctggcatgga cgccgaaacg gccgcccggg ccgtcgagcc cttcttcacc
      601 accaagccgc ccggcgaggg cacaggcctt ggcctgtcgc aggtgtatgg cttcgcgcgg
      661 cagagcggcg gctcggtgga tatcgagacc cgccccggcc agggcgcgac tatcgccatg
      721 ctgctgccga tcgccgcggc gaccgatccg gacgccagcg aggccgcttt cgaccacgtg
      781 gccggccagg ttccggctcg cgtgctgctg gtcgaggacg atccccaggt ggccgagctg
      841 gttgacgcca tgctgcatga cctgggccac acggtggttc gggccggcgg cgtcgacgag
      901 gccctcgacc gcctgaacgc ggacaagacc atcgagctgg tgctcagcga cgtgatcatg
      961 cccggcgggc gcagcggcgt ggacctggcc gaacacttgg cgacggcccg ccccggcctc
     1021 ccggtggtgc tgtgctccgg ctacaccggc ggcgaccagg gccgcgccca ggccggcgcc
     1081 tggcccttcc tgagcaagcc gttctcgctg gacagcctgg ccagggtgct gaccgaggcg
     1141 cgcgtgagat agagggctcg ggcggtctct ccaaaccgac cctttgcatt ctcatt
//
LOCUS       DAOMEO010001146         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11774_length_1194_cov_2.230904, whole genome shotgun sequence.
ACCESSION   DAOMEO010001146 DAOMEO010000000
VERSION     DAOMEO010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11774_length_1194_cov_2.230904"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1194)
                     /locus_tag="PLH31_19220"
     CDS             complement(<1..>1194)
                     /locus_tag="PLH31_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQR91359.1"
                     /translation="RQHVWPRGKVLGGSSSINAMLYVRGQQADYDGWRQLGCEGWAWD
                     DVLPWFRKSQNQERGACDLHATGGPLNVSDMRDGNPISDALIEACDQAGIPRYDDLNG
                     KDQEGATWYQVTQKNGARCSAAVAYLHPAMKRANLQVETGALASRVIFEGRRAAGVEF
                     VQNGVKRTARARAEVILAGGAVNSPQLLQLSGIGNGELLRQHGVAMVCDLPGVGENLQ
                     DHYLTGVRYRLKAGAASINAMSKGGRLAAEALKYVLFRKGLLTLSAAHVAAFCKSRPD
                     LAGPDIQFHILPATMDLDKLFNQQKMELEGAPGMTIAPCQLRPESRGHIRIKSADPSD
                     YPAIFANYLSDPLDQEVAIAGLKWARKIGEQPAIARYVDHEMNPGPGFESDEMLLEYA
                     RASGST"
BASE COUNT          176 a          400 c          411 g          207 t
ORIGIN      
        1 gtcgagcccg aggcgcgggc atattccagc agcatctcgt cgctctcgaa gcccggaccc
       61 gggttcatct cgtgatcgac atagcgggcg atggcgggct gctcgccgat cttgcgggcc
      121 catttcaggc cggcgatggc gacttcctgg tccagcggat cggacaggta gttggcgaag
      181 atggccggat agtcggacgg atcggccgac ttgatgcgga tatggccccg gctttcggga
      241 cgcagctggc agggggcgat ggtcatgccc ggcgcgcctt ccagctccat cttctgctgg
      301 ttgaacagtt tgtccagatc catggtggcc ggcaggatgt ggaactggat gtccggccct
      361 gcgagatccg gcctggattt gcaaaacgcg gcgacgtggg ccgccgacag ggtcagcagg
      421 cccttgcgga acagcacata tttcagcgcc tcggcggcca ggcgaccgcc cttgctcatc
      481 gcgttgatcg acgcggcgcc ggccttgagg cgatagcgga cgccggtcag atagtggtcc
      541 tgcaggttct cgccgacgcc ggggaggtcg cacaccatgg cgaccccgtg ctggcgcagc
      601 agctcgccat tgccgatgcc cgacagctgc agcagctgcg gcgagttcac cgccccgccc
      661 gccagaatga cctcggccct ggccctggcg gtgcgcttga cgccgttctg gacgaactcg
      721 acgccggcgg cgcgcctgcc ttcaaagatc acgcggctgg ccagggcgcc cgtctcgacc
      781 tgcaggttcg cacgcttcat ggccgggtgc agataggcga cggcggccga gcagcgcgcg
      841 ccgtttttct gggtcacctg gtaccaggtg gcgccttcct gatccttgcc gttcaggtcg
      901 tcatagcggg gaatgccggc ctggtcgcag gcctcgatca gggcgtccga gatcgggttg
      961 ccgtcgcgca tgtcggagac gttcagcggg ccgcccgtgg cgtgcaggtc gcaggccccg
     1021 cgctcctggt tctgggattt gcggaaccac ggcagcacat cgtcccaggc ccagccctcg
     1081 cagcccagct gccgccagcc gtcatagtcg gcctgctggc cgcgcacata cagcatggcg
     1141 ttgatcgagg acgagccgcc cagcaccttg ccgcgcggcc acacatgctg gcgc
//
LOCUS       DAOMEO010001147         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11776_length_1194_cov_1.913960, whole genome shotgun sequence.
ACCESSION   DAOMEO010001147 DAOMEO010000000
VERSION     DAOMEO010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11776_length_1194_cov_1.913960"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..809)
                     /locus_tag="PLH31_19225"
     CDS             complement(72..809)
                     /locus_tag="PLH31_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HQR91360.1"
                     /translation="MFDETTPRLGLPYVVAAQAQKHIPINEGLARLDALVQLAVESRV
                     VAAQPASPVAGGVWILPAAATGAAWAGQPTGTLMRFEAGAWEAVPLAEGVLTWVKDEN
                     QMLAFDGAAWIPLSTTFKSLTAAASPGLANTRLEIREEETTVSGATTSTAMVIPARAI
                     VLAVSTRTTVAVTGATSYSCGVAGEAGKFGSSLGVAKNASNVGVVGPTAYYADTPVLL
                     TSAGGNFTGGKVRVAVHLLRFDAPAAV"
     gene            complement(915..>1194)
                     /locus_tag="PLH31_19230"
     CDS             complement(915..>1194)
                     /locus_tag="PLH31_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQR91361.1"
                     /translation="NYLRPSVVKAGLDPENLPVSDPSAMNFGSGGNQEAKAWRDIWGC
                     GQGIGAIDDVLSAGDLVAKFAAEYEQAKAELAAKTALTSGHHLAFAAE"
BASE COUNT          181 a          436 c          394 g          183 t
ORIGIN      
        1 gccagcacgg cataagccgc attagccccc tcagtcgctc cgcgacagct ccccccagag
       61 ggggagcagc tttagaccgc cgctggcgca tcaaaccgca gcagatgcac cgctacccgc
      121 accttgccgc cggtgaaatt gccgccggcc gaagtcagca acaccggcgt atcggcgtag
      181 taggccgtcg gcccgaccac cccgacattg ctcgcgttct tcgcaacgcc cagcgacgag
      241 ccaaactttc ccgcctcgcc agccacgccg cagctgtacg acgtcgcccc cgtcaccgcg
      301 accgtcgtcc gggtcgagac ggccagcacg atggcccgcg ccggtatgac catcgccgtc
      361 gaggtggttg cgcccgagac cgtcgtctcc tcctcgcgga tctccagccg ggtattggcc
      421 aggcccggcg aggcggcggc ggtgaggctc ttgaaggtgg tcgacagcgg gatccaggcc
      481 gcgccgtcga aggccagcat ctggttctcg tccttgaccc aggtcagcac gccctcggcc
      541 aggggaacgg cctcccaggc gccggcctcg aaccgcatca gggttccggt cggctgaccg
      601 gcccaggccg cgccggtcgc ggcggccggc aggatccaga cgccgcccgc gacgggactg
      661 gccggctggg cggcgaccac acggctttcc accgccagct gaacaagggc gtccagccgg
      721 gcgaggcctt cattgatggg gatgtgtttc tgggcctggg cggcgaccac atagggcagg
      781 cccaggcgcg gggtggtttc gtcgaacatc ggagcatcct tcaaggcgtt gaactcgcct
      841 ctaggatcac cccgctgaca gagccgggga ccgaccgtgg tcgatccccg tcccttcccc
      901 gctccagcgt gtcgctactc ggccgcgaag gccagatggt ggcccgatgt cagggccgtc
      961 ttggcggcca gttcggcctt ggcctgctcg tactccgcgg cgaacttggc gacgaggtcg
     1021 ccggcgctca gcacgtcgtc gatggcgccg atgccctggc cgcagcccca gatatcgcgc
     1081 caggccttgg cttcctggtt gccgcccgag ccgaagttca tggccgacgg atcgctgact
     1141 ggcaggttct ccgggtccag gcccgccttg accaccgagg gacgcagata gttg
//
LOCUS       DAOMEO010001148         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11803_length_1192_cov_2.095866, whole genome shotgun sequence.
ACCESSION   DAOMEO010001148 DAOMEO010000000
VERSION     DAOMEO010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11803_length_1192_cov_2.095866"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLH31_19235"
     CDS             complement(<1..926)
                     /locus_tag="PLH31_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91362.1"
                     /translation="MIWNIIDRRERLCGFKCSLSLGLVIALVGGLAACEKYDPIEGDP
                     ERALLVEADILYDGKPLRLAMRTDCQVPKAYMSSVPIQAYPDVVAASLPDGSVIAFSG
                     MTYICRAARMEAGRQIQALPGGRRATDTDTILKGARALPPLTATWFSAKVRPEIFDEY
                     LTPTPTAGRYRPVAFRYRQVTPSIEEPTPQRVLEETLPAWRAMGPNERRGARYCGGKV
                     ESFPLTGFASKAGLLSAGRGFAAYSYDNNPHGRRFFPDIGPEGSFEETGGIERPGATA
                     AAVQLQERRARRERLKTFVRGPNGWSAESIQV"
BASE COUNT          234 a          399 c          343 g          216 t
ORIGIN      
        1 atcacttgaa tggactcggc gctccagccg ttggggccac ggacaaatgt tttcagccgc
       61 tcacgccgcg cccggcgctc ctgcagctgc acagctgcgg ctgtcgcccc cggacgttcg
      121 atccctccgg tttcctcaaa acttccctca ggccctatgt cggggaagaa ccgacgaccg
      181 tgagggttgt tgtcatatga ataggccgca aagccccgcc ccgccgacag caaacccgct
      241 ttggaggcga agcccgtcaa cggaaaactt tccacctttc cgccgcagta acgggccccg
      301 cgcctttcgt ttggccccat cgccctccat gcgggaagtg tttcctccag aactcgctgc
      361 ggcgttggct cttcaataga aggcgtgacc tgacgatatc gaaacgcgac aggtcgatag
      421 cgacccgcgg tcggcgtcgg agtcagatac tcgtcaaaga tttcgggcct gacctttgcg
      481 gaaaaccatg tggcggtcag cggaggaagc gctcgcgcgc ccttcaaaat ggtatcggtg
      541 tcagtggcgc gacgccctcc gggaagggcc tggatctgac ggccagcttc catccgcgct
      601 gctcgacaga tgtaagtcat accgctgaag gcgatgacac tgccgtcggg caagcttgcg
      661 gcgaccacgt ctggataggc ttggatcggg acgctcgaca tgtaggcttt cggaacctga
      721 cagtcggtcc gcatggcaag acgaagaggc ttgccgtcgt agaggatgtc ggcctccacc
      781 agcagcgccc tctcggggtc accctcgatg gggtcgtatt tttcgcaagc cgccagcccc
      841 ccgactaggg cgatgaccaa gccgagagaa agcgagcact tgaacccaca gaggcgctcg
      901 cgccgatcga taatgttcca gatcatccct tagagtggga aaatgaggac cgcagggcaa
      961 cgtctgtttc ccacctacaa cagactctcg ccacgtccga tcttgtccag cgtcccccgc
     1021 ccgccgctag actccccacc ttccacctgc tacacgccgc gcctcgctcc aggactccgt
     1081 cctgcgggcg cgttcgtcgt tatcggttcg agagcggcga aatcaaatcc gtggaaggga
     1141 cgccaagccc gcaccacggc tcaccagcga gggctcaccg ccctcacgag ag
//
LOCUS       DAOMEO010001149         1190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11824_length_1190_cov_2.320705, whole genome shotgun sequence.
ACCESSION   DAOMEO010001149 DAOMEO010000000
VERSION     DAOMEO010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11824_length_1190_cov_2.320705"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLH31_19240"
     CDS             <1..582
                     /locus_tag="PLH31_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HQR91363.1"
                     /translation="AVRTGDLDIAQDYGVSIALDDALEVGFLEVLRSADPAYSPVPKL
                     DPGKSATYRTPDGYAVDVLTTSRDAGDAETSRLAALKTDATPLRFMDFLLRDTIDAVV
                     LHEDGILIRVPAPARYAVHKLMISRKRSQANPKRRKDLEQAKALIHALSADDPFALRE
                     AYAEARGRGEVWRKLLDEAVTLLPETARVALDA"
     gene            639..>928
                     /locus_tag="PLH31_19245"
     CDS             639..>928
                     /locus_tag="PLH31_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR91364.1"
                     /translation="MREVAYADIASLVGQEIGVSDWVEISQERVNLFAEATGDHQWIH
                     VDVERATKEIGGPIAHGYLTLSLIPMLGAGILRVTGVTRGINYGCDKVRFTG"
     assembly_gap    929..938
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <939..1100
                     /locus_tag="PLH31_19250"
     CDS             <939..1100
                     /locus_tag="PLH31_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR91365.1"
                     /translation="MVRVGKKVRMRQKLLSVEAKAGGLQMKNECTIEIEGEERPACVA
                     ETISIIYGA"
BASE COUNT          213 a          403 c          385 g          179 t
ORIGIN      
        1 gccgttcgca ccggcgatct cgacatcgcc caggactatg gcgtgtcgat tgccctggac
       61 gacgcgttgg aggttggctt cctggaggtg ctgcgtagcg ccgacccggc ctactcgccg
      121 gtccccaagc tggaccccgg caagagcgcg acctaccgca cgcctgatgg ctacgcggtc
      181 gatgtgctga ccaccagccg cgatgcaggc gacgccgaga cctcaagact ggccgcactg
      241 aagaccgacg caacccctct gcgcttcatg gactttctgc tgcgcgacac gatcgacgcc
      301 gtcgttctgc acgaggacgg catcctgatc cgcgtgcccg ccccggcccg ctacgccgtc
      361 cacaagctga tgatctcgcg caagcgcagt caggccaatc ccaagcgccg caaggacctg
      421 gaacaggcca aggcgctgat ccacgccctc tcggccgacg accccttcgc cctgcgcgag
      481 gcctatgccg aggcgcgcgg acgcggcgag gtctggcgca agctgctgga cgaggcggtc
      541 acgctgctgc ccgaaaccgc tcgcgtcgcg ctggacgcgt gacagccggc gcgcggcccg
      601 ctacgacttt caaaacaact gtttgaaggg agcaagccat gcgcgaagtc gcctatgcgg
      661 atatcgccag cctggtcggt caggaaatcg gcgtttcgga ctgggtcgag atcagccagg
      721 agcgggtcaa cctgttcgcc gaggccaccg gcgaccacca gtggatccac gtggatgtcg
      781 agcgcgccac gaaggaaatc ggcggcccga tcgcccacgg ctatctgacc ctgtcgctga
      841 ttccgatgct gggcgccggc atcctgcggg tcaccggcgt gacgcgcggc atcaactatg
      901 gctgcgacaa ggtccgcttt accggcatnn nnnnnnnnat ggtccgggtc ggcaagaagg
      961 tgcgcatgcg ccagaagctg ctctccgtcg aggccaaggc cggcggcctg cagatgaaga
     1021 acgaatgcac catcgagatc gagggcgagg aacgccccgc ctgcgtggcc gagaccatct
     1081 cgatcatcta cggcgcatag agatcctagc gtcccgccac ggcccgcaag gcggcggccg
     1141 tggcggggtc ggccggcagg aaggtctcga tcgtcagttc cgaaatggtc
//
LOCUS       DAOMEO010001150         1189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11842_length_1189_cov_2.318342, whole genome shotgun sequence.
ACCESSION   DAOMEO010001150 DAOMEO010000000
VERSION     DAOMEO010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11842_length_1189_cov_2.318342"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLH31_19255"
     CDS             <1..665
                     /locus_tag="PLH31_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQR91366.1"
                     /translation="LAIFAALASTIATQGLGMLATYGKFVLGFYLTMGVLWLLLFIAG
                     ALVLGKRVVPLFGVIRDPTLLAFSTASSEAAYPRILDSLPKVGVRRRIVSFVLPLGYS
                     FNLDGSMLYCTFATMFIMQAHGVELSVSQQIFMLLLLMVTSKGIAGVPRASLVVIMAT
                     LTYFGLPEAWIALVLGVDHLLDMGRSATNVVGNSVAAAVVAKWEGELDDMPDEDPEPA
                     KA"
     gene            735..1142
                     /locus_tag="PLH31_19260"
     CDS             735..1142
                     /locus_tag="PLH31_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91367.1"
                     /translation="MSRKIAGLTAIAALMAAAAPALAEDRACFNSREVKNWSAMGDQT
                     LLLRVRTSEYYRIDLSRPVKHIRSPAALLALVSREQSQICNAGDLDPRIMLGPRFSLG
                     LPVAGLHRLTEAEQAAMGETNLPGRHYRRRNQD"
BASE COUNT          174 a          430 c          367 g          218 t
ORIGIN      
        1 cgctggccat cttcgcggcc ctggcctcga ccatcgccac ccagggcctg ggcatgctgg
       61 ccacctatgg caaattcgtg ctgggcttct acctgaccat gggcgtgctg tggctgctgc
      121 tgttcatcgc cggcgccctg gtgctgggca agcgggtggt tccgctgttt ggcgtgatcc
      181 gcgaccccac cctgctggcc ttctcgaccg ccagctcgga agccgcctat ccgcgcatcc
      241 tcgacagtct gcccaaggtg ggcgtgcgcc gccgcatcgt ctcgttcgtg ctgccgctgg
      301 gctattcgtt caatctcgac ggttcgatgc tgtactgcac cttcgcgacc atgttcatca
      361 tgcaggcgca cggggttgag ctgtcggtgt cgcagcagat cttcatgctg ctgctgctga
      421 tggtgacgtc caagggcatc gccggcgtgc cgcgcgcatc cctggtggtg atcatggcca
      481 ccctcaccta tttcggccta cccgaagcct ggatcgccct ggtgctgggc gtcgaccacc
      541 tgctggacat gggccgctcg gccaccaacg tggtcggcaa cagcgtcgcc gccgccgtgg
      601 tcgccaaatg ggagggcgag ctggacgaca tgcctgacga ggatccagag cccgccaagg
      661 cctgacgact caaggttgac ttgcggcgga aggggtccat gctcgattgc accctttccg
      721 ccggagttcc cgccatgtct cgcaagatcg ccggtctgac cgccatcgcc gcgctgatgg
      781 ccgccgccgc gccagccctc gccgaagatc gcgcctgctt caacagtcgc gaggtcaaga
      841 actggtccgc catgggcgac caaaccctgc tgctacgcgt gcgcacgtcg gaatattacc
      901 ggatcgacct cagccggccg gtcaagcaca tccgctcgcc cgccgccctc ctggcgctgg
      961 tcagccgcga gcagagccag atctgcaacg ccggcgacct ggacccccgc atcatgctcg
     1021 ggccgcggtt ctcgctgggc ctgcctgtcg ccggcctgca ccggctcacc gaggccgagc
     1081 aggccgccat gggcgagacc aacctgcctg gccggcacta tcgccgccga aatcaggact
     1141 gagacgacct cgccattgac ttgagcgcgc gtcgggccaa tctcctccc
//
LOCUS       DAOMEO010001151         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11862_length_1188_cov_2.325684, whole genome shotgun sequence.
ACCESSION   DAOMEO010001151 DAOMEO010000000
VERSION     DAOMEO010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11862_length_1188_cov_2.325684"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..>1188)
                     /locus_tag="PLH31_19265"
     CDS             complement(347..>1188)
                     /locus_tag="PLH31_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91368.1"
                     /translation="PEVFSYASFARGYKGGGYNLDRTQSSNGLQSGGQGVLPIYDTSF
                     AGEFVDSYEVGLKNTLFNRSVLLNVTGFYQKFTDFQLNTFLGTSFAVRSIPEVVSQGL
                     DADFLWFTPVRGLTVQSGFTWAKTEYGDQKIPNDPTNALALLPGSTLSLAPEYSASGS
                     LTYERPVGDNYKARFNVGAKYSSEYNTGSDLFPPKLQESFTVVNARIGFGAASEAWTV
                     ELWGQNILDEEYTQVAFNGFLQGGSGLSATNTTYVPANDTITYDAFLGAPRTYGVTLR
                     AKF"
BASE COUNT          213 a          352 c          410 g          213 t
ORIGIN      
        1 gagccggtcg ggtgcgggcg ctgggccatg gcgcgcacat cgtccatggc gcgcgcggcg
       61 gagaagaccg cggtcggcgc ggacgtatcg acgggctggg gcgttctctg ggccagggcg
      121 ccgagagcca caccgagcgt caggcagatc cagaagccca gtatcctgat cgtcttcacg
      181 gtctgctccc ggtccggcga atccccatca agaggttgcg tcgtgtcggg caggatcgga
      241 agtccaaaag aaaaagggcg acccggttgc ccggatcgcc ctttcttcat ccgaaagcca
      301 aggaccctgc tcgctttgga gagcgggtcc ctggatcccc tcccggttag aacttcgccc
      361 gcagcgtcac gccgtaggtg cgtggcgcgc cgaggaaggc gtcataggtg atcgtgtcat
      421 tggccggcac gtaggtcgtg ttggtggcgc tcaggcccga cccaccttgc aggaagccgt
      481 tgaacgcgac ctgggtatat tcctcgtcca ggatgttctg accccacagt tcgacggtcc
      541 aggcctcgct ggcggcgccg aagccgatgc gggcgttgac cacggtgaag ctttcctgaa
      601 gcttcggcgg gaacaggtcc gaaccggtgt tgtattccga cgagtacttg gcgccgacat
      661 tgaagcgggc cttgtagttg tcgcccaccg gacgctcata ggtcagcgaa cccgaggccg
      721 aatattccgg cgccagcgac agggtgctgc cgggcagcag ggccagggcg ttggtcggat
      781 cgttcgggat cttctgatcg ccatactcgg tcttggccca ggtgaagccg ctctggacgg
      841 tcaggccgcg aaccggggta aaccacagga agtcggcgtc caggccctgc gaaaccactt
      901 ctgggatcga gcgaacggcg aacgaagtgc ccaggaaggt gttcagctgg aagtcggtga
      961 acttctggta gaagcccgtg acgttgagca gaaccgaacg gttgaacagg gtgttcttca
     1021 ggccgacttc gtagctgtcg acgaactcgc cggcgaacga ggtgtcatag atcggcagca
     1081 cgccctggcc gccgctttgc aggccgttgg acgattgggt gcggtcgagg ttgtagccgc
     1141 cgcccttgta gccgcgcgcg aacgaggcgt agctgaacac ttcaggcg
//
LOCUS       DAOMEO010001152         1187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11870_length_1187_cov_2.380742, whole genome shotgun sequence.
ACCESSION   DAOMEO010001152 DAOMEO010000000
VERSION     DAOMEO010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11870_length_1187_cov_2.380742"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1187)
                     /locus_tag="PLH31_19270"
     CDS             complement(<1..>1187)
                     /locus_tag="PLH31_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR91369.1"
                     /translation="FIGFRGVGADVTAARAAQERISRLAHFDVLTQLPNRLSFLQALS
                     KAWTDHADPGADASGGCAVLCLDLDYFKGVNDSLGHPVGDALLIEVAERLRDTVADTG
                     LVARLGGDEFAVVIAPAPAPDILGDLSRMIVETLSQPYEIKGHNILIGASIGIAIAPA
                     DAADPDSLLKNADLALYRAKGDGRGAYRFFEVSMDVWAQERRALEIDLRAALKNEELK
                     VFFQPLIGCREHNITGFEALLRWQHPRRGLVPPSDFIECAEQWGLIGKIGEWVLNEAC
                     RQAASWPAPLTVAVNLSPNQFASGELVGQVAHALKLSGLDPSRLELEITEGLLLSDTA
                     RTMEQLAALKALGVKIAMDDFGTGYSSLAYLWRFPFDKIKLDRSFVAEMSDNAAITDI
                     VRT"
BASE COUNT          207 a          379 c          410 g          191 t
ORIGIN      
        1 atggtgcgga cgatgtcggt gatggcggcg ttgtcgctca tctcggccac gaacgagcgg
       61 tcaagcttga tcttgtcgaa cgggaaccgc cacagatagg caaggctgga atagccggtg
      121 ccgaaatcgt ccatggcaat cttcacaccg agcgccttga gcgccgccag ctgttccatc
      181 gtccgggcgg tgtcgctcag gagcagaccc tcggtgattt cgagctccag gcgcgacggg
      241 tccagccccg aaagcttcag cgcgtgggcg acctggccga caagctcgcc cgaggcgaac
      301 tggttgggcg acagattgac ggccacggtc aggggcgccg gccacgaggc ggcctgccga
      361 caggcttcgt tgagcaccca ctcgccgatc ttgccgatca ggccccactg ttccgcgcat
      421 tcgatgaagt cgctgggcgg gaccagtccg cgacgcgggt gctgccagcg cagcaaggcc
      481 tcgaagcctg tgatgttgtg ctcgcgacag ccgatcagcg gctggaagaa gaccttcaat
      541 tcttcattct tcagggcggc gcgcaggtcg atttccagag cccggcgctc ttgcgcccag
      601 acatccatcg acacctcgaa gaagcgatag gccccgcgac cgtcgccctt ggcgcgatag
      661 agggccaggt cggcgttctt cagcaggctg tcgggatctg cggcgtcggc cggagcgatg
      721 gcgatgccga tgctggcgcc gatcaggatg ttgtggccct tgatctcgta gggctgggac
      781 aaggtctcga cgatcatgcg ggacaggtcg cccaggatgt ctggcgccgg ggcgggagcg
      841 atcaccacgg cgaactcgtc gccgcccagt cgggcgacca ggccggtgtc ggccaccgtg
      901 tcgcgcaggc gctcggcgac ctcgatcagc agggcgtcgc ccaccgggtg gcccaggctg
      961 tcattgacgc ccttgaaata gtcgagatcc aggcacaaca ccgcgcaacc cccagaggcg
     1021 tctgcaccag ggtcggcatg gtcggtccag gccttgctga gcgcctgcag gaaggacagt
     1081 cggttgggca gctgggtcag gacgtcgaaa tgggccaggc gcgagatgcg ctcctgcgcc
     1141 gcccgggcgg cggtgacatc ggcgcccacg ccccggaaac cgataaa
//
LOCUS       DAOMEO010001153         1186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11882_length_1186_cov_2.288240, whole genome shotgun sequence.
ACCESSION   DAOMEO010001153 DAOMEO010000000
VERSION     DAOMEO010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11882_length_1186_cov_2.288240"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..898
                     /locus_tag="PLH31_19275"
     CDS             134..898
                     /locus_tag="PLH31_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HQR91370.1"
                     /translation="MTSPPPDLDAWRAAMTKPAGPLGGLARKAQVKINQIIPEKVHAA
                     ITTAMEGLTRGILTGADFATGSPQHGLSLDEKRAKAAAAIDGWKKTASVEGGITGAGG
                     FLAAAADFPLLMSFKIKLLFEIAAIFGHDGTDLDERLYILHIFELAFSDAGHRARVLA
                     AMDNWDARRAERPDTLETFDWRAFQQQYRDHIDLAKLAQMLPVVGAPVGAIVNWRLVE
                     SLGHTAVMAYRMREEGLVSGPATGIAIAPSPDGRGD"
BASE COUNT          200 a          403 c          416 g          167 t
ORIGIN      
        1 ggatgagggg ttgatggcga gggccgccgc aacgggcgga tatgcggggt tcggcgccgg
       61 cggtgcgacc cctcatccgt cggcctgcgg ccgccacctt ctcccgcaag gggagaaggg
      121 ttatctcagg cccatgacct caccaccccc cgacctcgac gcctggcgcg ccgccatgac
      181 caagcccgct ggccccctcg gcggcctcgc gcgcaaggcg caggtgaaga tcaaccagat
      241 catccccgag aaggtccacg ccgcgatcac cacggccatg gagggcctga cgcggggtat
      301 tctgaccggg gccgacttcg ccaccggctc gccgcagcac ggcctgagcc tggacgagaa
      361 gcgcgcgaaa gccgccgccg ccatcgacgg ctggaagaag accgccagcg tcgagggcgg
      421 gattaccggg gccgggggct ttctggcggc ggcggccgac ttcccgctgc tgatgagctt
      481 caagatcaag ctgctgttcg agatcgccgc catcttcggc catgacggga ccgacctgga
      541 tgagcggctc tatatcctgc acatcttcga gctggccttc tcggacgctg gccaccgggc
      601 ccgggtgctg gcggccatgg acaactggga cgcccgccgg gcggagcgcc ccgacacgct
      661 ggagaccttc gactggcggg ccttccagca gcagtaccgc gaccacatcg acctggccaa
      721 actggcccag atgctgcccg tggtcggcgc gccggtcggg gcgatcgtca actggcggct
      781 ggtcgagagc ctggggcaca cggcggtgat ggcctaccgg atgcgggagg aggggctggt
      841 ctcggggcca gcgacgggga ttgcaatcgc gccgtccccc gatgggcgcg gggattagaa
      901 accctctccc agagggagag ggaggggccc gccgcgcagc ggtgggaggg tgaggggttt
      961 gcgaagccct tccagacaag gcacgatccc aggcgcgaca acggtttgcg gtgtaacccc
     1021 tcaccctccc acgcaagcgt gggtccctcc ctctccctct gggagagggt cgatggaacg
     1081 gggaccggca cgggcgtcca gcaaacttat ccgacgcgtt caaacctccc atattcttga
     1141 cccacctcgt cgcgaggaaa gggcgcaagg ccagcgacca ttgcgg
//
LOCUS       DAOMEO010001154         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11899_length_1185_cov_2.342478, whole genome shotgun sequence.
ACCESSION   DAOMEO010001154 DAOMEO010000000
VERSION     DAOMEO010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11899_length_1185_cov_2.342478"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..382)
                     /gene="nirD"
                     /locus_tag="PLH31_19280"
     CDS             complement(29..382)
                     /gene="nirD"
                     /locus_tag="PLH31_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HQR91371.1"
                     /translation="MNAPVKQPVWIDVGAVTDVPRRGARRVTSPRGDIAIFRTGDGEV
                     FALMDACPHKGGPLSQGIVHGRAVACPLHNWSIDLASGEPMGADKGKGCTPVVPLEVR
                     DGRLLLSLALVEADG"
     gene            complement(379..>1185)
                     /locus_tag="PLH31_19285"
     CDS             complement(379..>1185)
                     /locus_tag="PLH31_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HQR91372.1"
                     /translation="RYINERVHANIQKDGTYSVVPRMWGGMTTPDELRAIADVADKFA
                     IPAVKVTGGQRIDLLGVKKDDLPAVWADLNAAGMVSGHAYAKGLRTVKTCVGSDWCRF
                     GTQDSTGLGIRLEKFLWGSWAPAKVKLAVSGCPRNCAEATCKDFGVVCVDSGFEIHIG
                     GAAGLHIQGTKVLTHVASEDEAVWVIAAAMQLYREEGWYLERVYKWMSRVGLEAIQAQ
                     VTDPEARKALYGRFVYSQRFARLDPWAERVAGRHNEEFTPMSRRMEFAPA"
BASE COUNT          188 a          405 c          403 g          189 t
ORIGIN      
        1 ggtcttgacc gaaagggcgg ccgcgccgtc agccatcggc ctcgaccaag gccaggctca
       61 gcagcagacg gccatcgcga acctccagcg gcacgaccgg cgtgcagccc ttgcccttgt
      121 cggcgcccat cggctcgccg ctggccaggt cgatcgacca gttgtgcagc gggcaggcca
      181 ccgcccgacc atggacgatg ccttggctca gcggcccgcc cttgtgcgga caggcgtcca
      241 tcagggcaaa gacctcgcca tcgccggtgc ggaagatggc gatgtcgccg cgcgggctgg
      301 tcacccggcg ggcgccgcgc cggggcacgt cggtgacggc gccgacgtcg atccagacag
      361 gctgtttgac gggggcgttc atgcgggagc gaactccatc cgacggctca tgggagtgaa
      421 ttcctcgtta tggcgaccgg ccacgcgctc ggcccacggg tcgaggcgag cgaagcgctg
      481 ggagtagaca aagcggccgt agagcgcctt gcgcgcttca ggatcggtga cctgggcctg
      541 gatggcctcc agccccacac gcgacatcca cttgtagacg cgctcgaggt accagccctc
      601 ttcgcgatag agctgcatgg cggcggcgat cacccagacg gcctcgtcct cggacgcgac
      661 atgggtcagc accttggtgc cctggatgtg caggccggcc gcgccgccga tgtggatctc
      721 aaacccgctg tcaacgcaga cgacgccgaa gtctttgcag gtggcctcgg cgcaattgcg
      781 cggacagccc gatacggcca gcttgacctt ggccggcgcc cacgagcccc acaggaactt
      841 ctccagacgg atgcccagac cggtggagtc ctgggtgccg aaccggcacc agtcgctgcc
      901 gacacaggtt ttcaccgtgc gcaggccctt ggcgtaggcg tggccgctga ccatgccggc
      961 ggcgttcagg tcggcccaga cggccggcag atcgtccttc ttcacaccca gcaagtcgat
     1021 gcgctggccg cccgtcactt tgacggccgg gatggcgaac ttgtcagcga cgtcggcgat
     1081 ggcccgcagt tcgtcggggg tggtcatgcc gccccacatg cgtggcacga cgctgtatgt
     1141 gccgtccttc tggatgttgg catggacccg ctcgttgatg tagcg
//
LOCUS       DAOMEO010001155         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11928_length_1183_cov_2.515071, whole genome shotgun sequence.
ACCESSION   DAOMEO010001155 DAOMEO010000000
VERSION     DAOMEO010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_1183_cov_2.515071"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="PLH31_19290"
     CDS             complement(<1..>1183)
                     /locus_tag="PLH31_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91373.1"
                     /translation="EEVVVTGFRKSLADSTNAKRESVGFSDTVFAEDIGKFPDANIAE
                     SLNRIPGVQLTREINGEGLNVAIRGLGTNFTKVLLNGAQAAVASSGRTDSQNQNREVD
                     LDLYPTELFTRLDVSKTPTAGQVEGGVAGVVNMRSARPFDYKAAGQLTYSIQAGKNNQ
                     ADDISPRGSLIASKTWETNVGDFGILAGAAFARNKSTTTGFETIGWSNANMSCAGCNT
                     TGGNGFNWASTVPAGVTGNGLTPGQTVDNALLASLNPGTSLVQLSDALIPRLGRDSWS
                     SGERNRDSLIVSLEWRPNDALNLYFDTLYSKASREFERIDMNWVGRNSNFMVPVGVKV
                     DSNNVVTEGAFLNSQFFLEYRPYDEDVEVLNLNPGFTWRPNDWLKLDGQVNWSRSVFF
                     RE"
BASE COUNT          176 a          359 c          421 g          227 t
ORIGIN      
        1 cctcgcggaa gaagaccgag cggctccagt tgacctggcc gtccagcttg agccagtcgt
       61 taggacgcca ggtgaagccc gggttcaggt tcagcacttc gacgtcttcg tcatagggac
      121 ggtattcgag gaagaactgc gaattgagga aggcgccctc ggtgacgaca ttgttgctgt
      181 cgacctttac gcccaccggg accatgaagt tggagttgcg gcccacccag ttcatgtcga
      241 tccgctcgaa ctcgcggctg gccttcgagt acagggtgtc gaaatagagg ttcagggcgt
      301 cgttggggcg ccattccagc gaaacgatca gcgagtcgcg gttgcgctcg cccgagctcc
      361 agctgtcgcg gcccaggcgc gggatcaggg cgtcggacag ctgaaccagg ctcgtgcccg
      421 ggttcaggga cgccagcagg gcgttgtcga ccgtctggcc gggcgtcagg ccattgccgg
      481 tcacgccggc cggcacggtc gaggcccagt tgaagccatt gccgccggtg gtgttgcagc
      541 ccgcgcagct catgttggcg ttcgaccagc cgatggtctc gaagcccgtc gtggtcgact
      601 tgttgcgggc gaaggccgcg ccggccagga tgccgaagtc gccgacattg gtttcccagg
      661 tcttggacgc gatcagcgag ccgcgcgggc tgatgtcgtc ggcctggttg ttcttgccgg
      721 cctggattga ataggtcagc tggccggcgg ccttgtagtc gaacggccgg gcgctgcgca
      781 tgttgaccac gccggccacg ccgccctcga cctgtccggc cgtcggggtc ttggagacgt
      841 ccaggcgggt gaacagttcg gtgggataga ggtcaaggtc gacctcgcgg ttctggttct
      901 ggctgtcggt gcggcccgac gaggccacgg cggcttgcgc gccgttcagc agcaccttgg
      961 tgaagttggt gcccaggccg cggatggcga cgttcaggcc ctcgccgttg atttcgcggg
     1021 tcagctgcac gccggggatc cggttcagcg actcggcgat gttggcgtcc gggaacttgc
     1081 cgatgtcctc ggcgaagacg gtgtcgctga aaccgacgct ctcgcgcttg gcgttggtcg
     1141 agtcggccag gctcttgcgg aagccggtga cgaccacctc ctc
//
LOCUS       DAOMEO010001156         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11930_length_1183_cov_2.337766, whole genome shotgun sequence.
ACCESSION   DAOMEO010001156 DAOMEO010000000
VERSION     DAOMEO010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_1183_cov_2.337766"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PLH31_19295"
     CDS             complement(<1..111)
                     /locus_tag="PLH31_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HQR91374.1"
                     /translation="MTLFDSPDFEGHEGVHTFFDEKTGLKSIIAIHSTARG"
     gene            complement(172..>1183)
                     /locus_tag="PLH31_19300"
     CDS             complement(172..>1183)
                     /locus_tag="PLH31_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQR91375.1"
                     /translation="SGCAPLTQRLDLGPLGFRGPRFEGEDFVSFDGARLGLKTWLPDG
                     QPEWVIVGLHGMNDYSNAYHLAADWWTGQGIATYAFDVRGFGRSPERGVWAPVGMVIE
                     DIRACVTLVRERHPGAKVALAGISMGGGLAIAAMTDPDPPVVDRLLLFAPAVWGWSEQ
                     PLPNRASLWVTAHMVGGWVVEPPDWLVRKVMPSDNLDELRRMGKDPLMIWGARSDTVY
                     GLVGLMEKAWRSTDRITVPTAWFYGVNDKIIPKEPTVEAAARLKPSDRTACYEKGWHL
                     LLVDQQAETVWRDAVSFLRDPAAPWPSGAPPIPASVKLIEAMAPRPLKGGAKSSAKSS
                     GL"
BASE COUNT          201 a          409 c          394 g          179 t
ORIGIN      
        1 gccgcgcgcg gtggaatgga tggcgatgat cgacttcaga ccggtttttt cgtcgaagaa
       61 ggtatggacg ccttcgtgtc cctcgaaatc gggggaatcg aacaaggtca tgccgcaggt
      121 ctcgcctcag acagaaagaa tgcgccgggg tttacgctgc gcggcgggcg gtcacaaccc
      181 cgaagacttg gccgatgact tggcgccgcc cttgaggggc ctgggcgcca tggcctcgat
      241 cagcttgacg ctggccggga tcggcggcgc gcccgacggc cagggcgcgg cggggtcgcg
      301 caggaagctg accgcgtcgc gccagacagt ctcggcctgc tggtcgacca gcagcaggtg
      361 ccagcccttt tcgtagcagg ccgtccggtc ggaaggcttc agtctggccg ccgcctcgac
      421 cgtcggttcc ttgggaatga tcttgtcatt gaccccgtag aaccaggccg tcggaaccgt
      481 gatcctgtcg gtcgagcgcc acgccttctc catcaggcca accagcccat agacggtgtc
      541 ggaccgcgcg ccccagatca tcagcgggtc cttgcccatg cggcgcagct cgtcgagatt
      601 gtcgctgggc atcaccttgc ggaccagcca gtcgggcggc tcgaccaccc agccgccgac
      661 catgtgcgcg gtgacccaaa ggctggcgcg attgggcagc ggctgctcgg accagcccca
      721 gacggccggg gcgaacagca gcaggcgatc caccacaggc ggatcaggat cggtcatggc
      781 ggcgatggcc aggccgccgc ccatgctgat cccggccaag gctaccttcg cccccggatg
      841 ccgctcgcgg accagggtca cgcaggcgcg gatatcctcg atcaccatgc cgaccggcgc
      901 ccagacgccg cgctcaggcg agcggccaaa gccgcgcaca tcgaaggcgt aggtggcgat
      961 cccctgcccc gtccaccaat cagcggccag gtgataggcg ttggaatagt cattcatgcc
     1021 gtgcaggccg acgatcaccc attccggctg tccgtccggc agccaggtct tcaggcccag
     1081 ccgcgcgccg tcgaagctga cgaagtcctc gccctcgaag cgcggcccgc gaaagcccag
     1141 cggccctagg tcaagcctct gggtcagggg cgcgcaaccg ctg
//
LOCUS       DAOMEO010001157         1183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11935_length_1183_cov_1.910461, whole genome shotgun sequence.
ACCESSION   DAOMEO010001157 DAOMEO010000000
VERSION     DAOMEO010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_1183_cov_1.910461"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..>1183
                     /locus_tag="PLH31_19305"
     CDS             88..>1183
                     /locus_tag="PLH31_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin-coupled sensor protein"
                     /protein_id="HQR91376.1"
                     /translation="MSANPKLDQRMSFMRFDERDRSALRALRPLIDSEIANALNKFYG
                     QVRAFPEVRSKFRDDGHIAAAERAQANHWLRIATADYGPDYMRDVERIGRAHVVAGLE
                     PRWYMGGYAVVSEELVRAVVGKRARSLFANAKSDADVADSLVALNKAVFLDMELAISS
                     YLTLLEEQRATLEAERDAAEQAQAQAVKAIGEALSRLAAGDLSARVDGGLAPEFQSLK
                     DDFDRAAAALGEAMKAVERATSGIRAGADDIARNADDLAQRTEQQAATLEQTAAAVDQ
                     LTATVGKTALSAREVSARVGEASAEAERSGVVVTRAAEAMTKIEGSSNQVSQILGVID
                     EIAFQTNLLALNAGVEAARAGDAGRGFAVVA"
BASE COUNT          212 a          384 c          386 g          201 t
ORIGIN      
        1 atgtgcgcct gtcgcgtcag gcttcaatca tgattaacaa attgcgttca cgtctgatgc
       61 cgtaagtttc gagtcggagc caatgacatg agcgcgaacc ccaagctaga tcagcgcatg
      121 tccttcatgc ggttcgacga gcgcgaccgc agcgcgctgc gcgcgctcag gcccctgatc
      181 gactccgaga tcgccaacgc cctgaacaag ttctacggcc aggttcgcgc cttcccggaa
      241 gtacgaagca aattccggga cgatggtcac atcgctgcag ccgaacgggc ccaggccaat
      301 cactggcttc gaatcgccac ggccgactac ggccctgact atatgcgcga tgttgaacgc
      361 atcggtcgcg cgcacgtggt cgccgggctt gagcctcgct ggtatatggg cggttacgcc
      421 gtggtgtccg aagagctggt ccgcgccgtg gtcggcaagc gggccaggag tctgttcgcc
      481 aacgccaagt ccgacgccga tgtggcagat agcctggtgg cgctgaacaa ggccgtgttc
      541 ctcgacatgg aactggcgat ttccagctat ctgaccctcc tcgaagaaca gcgggcgact
      601 ctggaggccg aacgcgacgc cgctgaacag gctcaggccc aggcggtgaa ggccatcggc
      661 gaagccctgt cccgtctggc ggccggcgac ctgtcggcgc gggtcgatgg cggcctggct
      721 ccggagtttc agtctttgaa ggacgatttc gatcgcgcgg ccgccgccct tggcgaagcg
      781 atgaaagcgg ttgagcgcgc cacctcgggt atccgcgccg gcgctgacga tatcgctcga
      841 aacgccgatg atctggccca gcgcaccgag caacaggccg cgacgctgga gcagacggcc
      901 gctgccgtgg accagctgac cgccacagtt ggcaagaccg ccctcagcgc tcgtgaagtc
      961 tcagcccggg tgggcgaggc cagcgccgag gccgaacgct ctggcgtcgt cgtgacgcgc
     1021 gccgccgagg cgatgaccaa gatcgaaggc tcgtccaacc aggtttctca gatcctgggc
     1081 gtcatcgatg agatcgcgtt ccagaccaac ctgctggcgc tcaatgccgg cgtcgaagcc
     1141 gcccgggccg gcgacgcggg tcgcggtttc gctgttgtcg ccc
//
LOCUS       DAOMEO010001158         1182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11942_length_1182_cov_2.795918, whole genome shotgun sequence.
ACCESSION   DAOMEO010001158 DAOMEO010000000
VERSION     DAOMEO010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11942_length_1182_cov_2.795918"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLH31_19310"
     CDS             <1..465
                     /locus_tag="PLH31_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQR91377.1"
                     /translation="EWNEDEKRVDFSHNPFSMPQGGLEALETQDPLTIRAYQYDIVCN
                     GYELCSGAIRNHKPEIMLKAFEVAGYGAEVVEEQFGGMLNAFRFGAPPHGGLAPGIDR
                     IVMLLAEQVAIREVIAFPLNQQGQDLLMNAPAEAQDRQYKELHIRSAPPIKV"
     gene            complement(531..>1182)
                     /locus_tag="PLH31_19315"
     CDS             complement(531..>1182)
                     /locus_tag="PLH31_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR91378.1"
                     /translation="TRADMRGAEMRGANFAGANFTDARLSGVESSGANLTNANLTRAD
                     FSSSELHGANMVGANLSKANFSSAELTGSNLISVNARGADFQNAELNAANLTGARFDQ
                     ANLRNVELTGATLRGAIFHGADLRGADFSGAVLGGADLSGARGLDQDQLDEACGDGST
                     RLPRGLSVRSCHGDRRHIRVVVDFEHAKAQAAAARAAAAAARAVPKPPAPPKPPKY"
BASE COUNT          190 a          387 c          421 g          184 t
ORIGIN      
        1 gagtggaacg aagacgaaaa gcgcgtcgac ttctcgcaca acccgttctc gatgccgcag
       61 ggcggtctgg aggccctgga gacccaggat ccgctgacca tccgcgccta tcagtacgac
      121 atcgtctgca acggctatga gctgtgctcg ggcgcgatcc ggaaccacaa gccggagatc
      181 atgctcaagg ccttcgaagt ggccggctat ggcgccgagg tggtggaaga gcagtttggc
      241 ggcatgctga acgccttccg cttcggcgcc ccgccgcacg ggggcctggc ccccggcatc
      301 gaccggatcg tcatgctgct ggccgagcag gtggccatcc gcgaggtcat cgccttcccg
      361 ctgaaccagc agggccagga cctcttgatg aacgccccgg ccgaggcgca agatcgccag
      421 tacaaggaac tccacatccg ctcggcgccc cccatcaagg tctaaagcga atagaaaaag
      481 gccccggtct cgcgaccggg gcctttgaag ttttcggggg agcgtcgaac ctagtatttc
      541 ggcggtttgg gcggggccgg gggcttggga accgcccgcg ccgcagccgc cgcggctcgg
      601 gccgccgcgg cctgggcctt ggcgtgctcg aagtcgacca cgacccggat gtggcgccgg
      661 tcaccgtggc aggagcgcac cgacaggccg cgcggcaggc gggtggagcc atcgccgcag
      721 gcctcgtcca gctggtcctg gtccaggccc cgggcgccgg acaggtcggc cccgccgagg
      781 accgcgccgg agaaatcggc gcccctcagg tcggcgccgt gaaagatcgc cccgcgcagg
      841 gtggcgccgg tgagttcgac gttgcgcaga ttggcctggt cgaaccgggc gccggtcagg
      901 ttggcggcgt tcagctcggc gttctgaaag tcagcgcccc gggcgttgac cgagatcagg
      961 ttcgagccgg tcagctcggc gcttgagaaa ttggccttgc tcagattggc gcccaccatg
     1021 ttggcgccgt gcagttcgga ggacgaaaag tcggcgcggg tcaggttggc gttggtcagg
     1081 ttggcgccgc tgctctcaac acccgacagg cgggcgtcgg tgaaattggc cccggcgaaa
     1141 ttggcgccgc gcatctcggc tccccgcatg tcggcgcggg tc
//
LOCUS       DAOMEO010001159         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_11965_length_1181_cov_2.600355, whole genome shotgun sequence.
ACCESSION   DAOMEO010001159 DAOMEO010000000
VERSION     DAOMEO010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11965_length_1181_cov_2.600355"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..>590
                     /locus_tag="PLH31_19320"
     CDS             267..>590
                     /locus_tag="PLH31_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HQR91379.1"
                     /translation="MLRLPVAALIIACMATSALAQEREPEERQRLLDLAYTLGESHAL
                     RQTCEGVNDQLWRSRMVRLTEVEQADQGFDAQLRERFNTGFAARQGEFPLCDTASRKA
                     EQQSAR"
     assembly_gap    591..600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            765..>1181
                     /locus_tag="PLH31_19325"
     CDS             765..>1181
                     /locus_tag="PLH31_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HQR91380.1"
                     /translation="MVLLVLAILLVFNVIKIVPQGREFTVERFGRYTRTLKPGISFLT
                     PFFETIGRRVNMMEQVLDVPQQEVITKDNVSVKVDAIVFIQVMDAAQAAYKVDNLMYA
                     ITQLAQTNLRTVVGSMELDEVLSQRDQINTRLLSTID"
BASE COUNT          214 a          395 c          353 g          209 t
ORIGIN      
        1 gttcggccag acgagacgcc gcttcggtcg cctgatgctg ggtctcgtaa tgtccgaacc
       61 gcagatgctc gcccacgatg gaccagtagt tcccgacccg cacaacgcca taatgcactt
      121 cagccatgat cgacctcctt gcagtgaaac caccgttgcg ccgatccatg gctatcacaa
      181 ggcctcaaac gccacgcgat cgccatggac tcgcgcgaag ggcgcgtcgt cgtgacatcg
      241 caacacgaga cgggaatact gtcgtcatgc ttcgcttgcc tgtcgccgcc ctgatcatcg
      301 cctgcatggc cacctccgcc ctcgcccagg agcgggagcc ggaagagcgc cagcgcctgc
      361 tcgacctggc ctatacgctg ggcgagagcc acgccctgcg gcagacctgc gagggcgtga
      421 atgaccagtt gtggcgctcg cggatggttc gcctgaccga ggtggagcag gccgaccagg
      481 gcttcgacgc ccagctgcgc gaacggttca acacgggctt cgcggcgcgt cagggcgaat
      541 tccccctgtg cgacaccgcc agccgcaagg ccgagcaaca atcggcccgc nnnnnnnnnn
      601 ggcccgcaag ggtcaggccc tggcgctcaa gctctcgcag tccatgcgcg tggtccagcg
      661 cggcgagaca gcgccggatt ccatggcgga gatcgtcgcc ccgcgctaac ctacgcctgc
      721 tggttgtgta gggagcgctc ctgttgaccc catttgtctt tccgctggtc cttctggttc
      781 tggcgatcct gctggttttc aatgtcatca agatcgtgcc gcagggccgc gagttcacgg
      841 tcgagcggtt cgggcgttac acccgcacgc tgaaaccggg gatcagcttc ctgactccgt
      901 ttttcgagac gatcggccgt cgggtgaaca tgatggagca ggtcctcgac gttccccagc
      961 aggaggtcat caccaaagac aacgtctcgg tgaaggtcga cgccatcgtc ttcatccagg
     1021 tgatggacgc cgcccaggcc gcctacaagg tcgacaacct gatgtacgcc atcacccagt
     1081 tggctcagac caacctgcgc accgtagtcg gctcgatgga gctggacgag gtgctgagcc
     1141 agcgcgacca gatcaatacc cgcctgctgt cgaccatcga c
//
LOCUS       DAOMEO010001160         1177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12010_length_1177_cov_2.628342, whole genome shotgun sequence.
ACCESSION   DAOMEO010001160 DAOMEO010000000
VERSION     DAOMEO010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12010_length_1177_cov_2.628342"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..285
                     /locus_tag="PLH31_19330"
                     /pseudo
     CDS             1..285
                     /locus_tag="PLH31_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516122.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
     gene            325..582
                     /locus_tag="PLH31_19335"
     CDS             325..582
                     /locus_tag="PLH31_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91381.1"
                     /translation="MTIQVDIAKQNALAELVAHVRAGEDVVLTSDGQAIAVARPSEPQ
                     RVRKRTPGAWAHLGQLQDPDLFLRPDPDMEQAADGPIFPET"
     gene            579..959
                     /locus_tag="PLH31_19340"
     CDS             579..959
                     /locus_tag="PLH31_19340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR91382.1"
                     /translation="MTLLIDTHVLIWWLTEPSRLSSACLDRLGSGDVSVLVSAVSAYE
                     IEFKRCADPLLQRLPADLAHAVFAEGFDWLPITPEDAIIAGQLPRHHRDPWDRFLIAQ
                     GIRSQCPIMTRDRAFEAYEIELLW"
     gene            complement(966..>1177)
                     /locus_tag="PLH31_19345"
     CDS             complement(966..>1177)
                     /locus_tag="PLH31_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQR91383.1"
                     /translation="QSIIMTTIFWGGRGLGYFGDASRVDLWMIVAAIWVLQLIWSPLW
                     LSKFSMGPLEWVWRRLAYGKGLARA"
BASE COUNT          190 a          405 c          364 g          218 t
ORIGIN      
        1 gaagtggccg tcgccaccct gggcacggtc tatgcggtca gcgatccgga cggcgccacc
       61 acggccctgt agacggtgct gtccagccag tggtcgctgg cctcggccct gtcgttcctg
      121 gcctggtacg tcttcgcccc gcagtgcgcg cccaccctgg gcgtggtcaa gcgcgagacc
      181 aacagctggc tgtggccgac cgtgatgttc gtctacatgg tggccctggc ctatctggcg
      241 gcgtttgcga cctatcacac ggccgtggcg ctgggggcgg ggtaggggat cgtccggcgt
      301 caggccttgt gctaggctgg gcgcatgacg atccaggtcg acatagccaa acagaacgcc
      361 ctggcggaac tcgtcgccca tgttcgggcg ggcgaggacg ttgttctcac gtctgacggg
      421 caggccatcg cggtcgccag gccctccgag ccccagcgcg ttcgcaaacg cacgccgggc
      481 gcctgggcgc accttggcca acttcaggat cctgaccttt tcctgcgtcc tgatccggat
      541 atggaacaag ccgccgacgg cccgatcttt ccggagactt gacgcttctg atcgacacgc
      601 acgtgttgat ctggtggctc accgagccgt cgcggctctc gtctgcctgt ctggatcgtc
      661 tcggatcagg cgatgtgagc gttttggtca gcgccgtcag cgcctatgag atcgaattca
      721 agcgttgcgc cgacccgctg ctgcaacgct tgcccgccga tctcgcgcac gctgtcttcg
      781 cggaagggtt tgattggctg ccgatcacgc ccgaagacgc gatcatcgca ggccagttgc
      841 ctcgccatca tcgcgatccc tgggaccggt tcctgattgc ccagggcatc aggagccaat
      901 gtcccatcat gaccagagac cgcgcgtttg aggcctatga gattgagctg ctctggtagc
      961 ccgctctacg cccgcgccag ccccttgccg taggccagcc gccgccacac ccactccagc
     1021 ggacccatcg aaaacttcga cagccacagc ggcgaccaga tcagctgcag cacccagatc
     1081 gctgcgacga tcatccacag gtccacccgg ctggcgtcgc cgaaatagcc cagcccccgg
     1141 ccgccccaga agatcgtggt catgatgatc gactggg
//
LOCUS       DAOMEO010001161         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12020_length_1176_cov_2.522748, whole genome shotgun sequence.
ACCESSION   DAOMEO010001161 DAOMEO010000000
VERSION     DAOMEO010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12020_length_1176_cov_2.522748"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLH31_19350"
     CDS             complement(<1..1126)
                     /locus_tag="PLH31_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HQR91384.1"
                     /translation="MGLSPGQKAADALALVPDLVTADHDPQADRAALEALSDWCVRFS
                     PAVAIDGDDGLFLDITGTDHLWGGEAAMLADLLARLKRWGVPARAAIADTPGAAWALA
                     RFGEDGEIAPPGGQRALLADLPVAALRLDEMATAQLPRLGLFRIAQLLGLPRAQLARR
                     FGLHLTLRLDQALGAAGEALVFRRPANPWFDRLAFAEPISAPDDLARVVGDVTALICR
                     RLEAEGQGARRFEVVFHRLDGRAMPVRAGLARPGRDAKRLTRLLLPKLDVIDPGFGIE
                     VVTVFAGDVEPLTAVQARLDAEAGTGLDETLAPLVDRLINRLGDNRVWRADPHESHVP
                     ERSVIRRAPLASPPGQTWDRERPRPVRLFKRPEAITAMAKL"
BASE COUNT          167 a          444 c          421 g          144 t
ORIGIN      
        1 gcagcttggc catcgccgtg atcgcctcgg gccgcttgaa cagccgcacc ggtctggggc
       61 gctcgcggtc ccaggtctgg ccagggggcg aggccagcgg cgcgcggcgg atcaccgagc
      121 gctccggcac gtggctctca tgcggatcag cccgccagac gcgattatcg cccagccggt
      181 tgatcagccg gtcgaccagt ggggccaggg tctcgtccag ccctgttccc gcttcggcat
      241 ccagccgcgc ctggacggcg gtcaggggtt cgacatcgcc ggcaaacacc gtgaccacct
      301 cgatgccgaa accgggatcg atcacgtcca gcttggggag cagcagccgg gtcagccgtt
      361 tggcgtcccg tcccggccgc gccagcccgg cccgcaccgg catggccctg ccgtccaggc
      421 ggtggaagac cacctcaaaa cgccgcgcgc cctggccctc ggcctccagc cgtcggcaga
      481 tcagcgcggt gacgtcgccg accacccggg ccagatcgtc cggcgcgctg atcggctcgg
      541 cgaaggccag gcggtcgaac cacggattgg ccggacgtcg gaacaccaga gcctcgcccg
      601 ccgcgcccag ggcctgatcc agacgcaggg tcagatgcag gccaaaccgc ctggccagct
      661 gggcgcgggg caggcccagc aattgcgcga tccggaacag gcccagacgc ggcaactggg
      721 cggtcgccat ttcatccagc cgcagggccg ccacgggcag gtccgccagc agcgcgcgct
      781 ggccgcccgg cggggcgatc tcgccgtcct cgccaaaccg cgccaacgcc caggccgcgc
      841 caggcgtgtc ggcgatcgcc gcccgggccg gcacgcccca gcgctttagc cgggccagca
      901 ggtcggccag catcgccgcc tcgccgcccc acaggtggtc ggtcccggtg atgtcgagga
      961 acagaccgtc atcgccgtcg atggccacgg ccggcgagaa ccgtacgcac cagtcgctca
     1021 gggcctccag cgccgcgcgg tcggcctggg ggtcgtggtc ggcggtgacc aggtccggga
     1081 ccagcgccaa ggcgtcggcg gccttctggc cggggctgag acccagggcc tgggcggctt
     1141 cgtcgatggc ggccagccgg cgggttccgg attcgg
//
LOCUS       DAOMEO010001162         1176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12021_length_1176_cov_2.426405, whole genome shotgun sequence.
ACCESSION   DAOMEO010001162 DAOMEO010000000
VERSION     DAOMEO010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12021_length_1176_cov_2.426405"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..267
                     /locus_tag="PLH31_19355"
     CDS             10..267
                     /locus_tag="PLH31_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91385.1"
                     /translation="MRSAAWLAGALATSAAVVIGSALAVVFAATVVVIGFMGSALFGL
                     VALALRARRTVKAETGGDPSLIEARNVGGHSWVAYGWNERP"
     gene            363..653
                     /locus_tag="PLH31_19360"
     CDS             363..653
                     /locus_tag="PLH31_19360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HQR91386.1"
                     /translation="MIGVVATLKVQVDKTAEFEKVFLDLASKVKANEPGCLMYQLTKS
                     KTEAGTYKVLELYASMDDLKAHGGTDYFKAGGAAMGPFMAGRPEIEYLDAVE"
     gene            659..>1176
                     /locus_tag="PLH31_19365"
     CDS             659..>1176
                     /locus_tag="PLH31_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91387.1"
                     /translation="MDLAFSPEDLAFQQEVRDWIATAYDDDLRRKMSQSKNGYLDKAG
                     QVKWQKKLFERGWAAPDWPVEWGGCGFTASQRYIFNMELSLAGTPNPSPMGLKMCAPV
                     ILAFGTEEQKKQHLPPILSSDIWWCQGYSEPGSGSDLASLQMKAERDGDDYVLNGSKI
                     WTTHAQWADWMF"
BASE COUNT          200 a          355 c          398 g          223 t
ORIGIN      
        1 ggttcggctc tgcgtagcgc cgcctggctg gctggcgcgc tggcgacctc agccgccgtc
       61 gtgatcgggt cggctctggc cgtcgtgttt gccgccaccg ttgtggtcat cggttttatg
      121 ggcagcgcgc tgttcgggct cgtggcgctg gcgctccggg cgcgtcgcac cgtcaaggcc
      181 gagaccggcg gcgatccctc cctgatcgaa gcgcgcaatg tcggcggcca ttcctgggtc
      241 gcctacggct ggaacgagcg gccctaagct ctcacggttc ctttctgcct tccccaacgt
      301 cgcccttgcg gcgcgcgtcc gtgtggtcga tgctgctttc aaacaagcgt tgggaggcga
      361 acatgatcgg tgtggtagcg accttgaagg tccaagtcga caagaccgct gagttcgaaa
      421 aggtgttcct ggatctggcg tccaaggtga aggccaacga gccggggtgc ctgatgtacc
      481 agttgaccaa gtccaagacc gaagccggga cctacaaggt gctggagctc tacgcctcca
      541 tggacgacct gaaggcccac ggcggcaccg actatttcaa ggccggcggc gcagcgatgg
      601 ggccgttcat ggccggtcgt cccgagatcg aatatctgga cgcggtggag tagggaccat
      661 ggatctcgcc ttttcacctg aggatctggc cttccagcag gaagtccgcg actggatcgc
      721 cacggcctat gacgatgatc tgcgtcgcaa gatgagccag tccaagaacg gctatctgga
      781 caaggccggc caggtgaaat ggcagaagaa gctgttcgag cgcggctggg cggcgccgga
      841 ctggccggtg gagtggggcg gttgcggctt taccgcctcg cagcgctaca tcttcaacat
      901 ggaactgtcg ctggctggca cgcccaatcc ctcgccgatg ggcctgaaga tgtgcgcgcc
      961 ggtgatcctg gcgtttggca ccgaagagca gaagaagcag cacctgccgc cgatcctgtc
     1021 gtccgatatc tggtggtgcc agggttattc cgagcccggc tcgggctcgg acctggccag
     1081 cctgcagatg aaggctgagc gggacggcga cgactatgtg ctgaacggct ccaagatctg
     1141 gaccacccac gcccagtggg ccgactggat gttctg
//
LOCUS       DAOMEO010001163         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12060_length_1174_cov_2.362824, whole genome shotgun sequence.
ACCESSION   DAOMEO010001163 DAOMEO010000000
VERSION     DAOMEO010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12060_length_1174_cov_2.362824"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLH31_19370"
     CDS             complement(<1..422)
                     /locus_tag="PLH31_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91388.1"
                     /translation="MRLAWRGYRGSRSGKGGGALIGLGILFAFACLGGVLLALAVHGH
                     AVPIHPLAVLIADLLCAIVLTLMLSQTLGASAEALYERGDLDLLFSSPIAPEKVLLVR
                     SLGVATGVATIFMVFAAPLLLPSILYGHPGWLGVFGVL"
     gene            complement(422..1168)
                     /locus_tag="PLH31_19375"
     CDS             complement(422..1168)
                     /locus_tag="PLH31_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR91389.1"
                     /translation="MTEALIVRGLNKTFAKPAVVDLDLTVHAGELYALLGPNGAGKTT
                     TLRMVAGLTRPDTGEISVYGVDARQDPAGAKALVAWAPDEAMIYDRLTPLEYLEFVAG
                     LWNVDPALARTRAEELLAKLGLADEARRRCEGFSRGMKQKVALAGALIHDPRLLILDE
                     PLTGLDAAAARLVKDMLQARVDAGGTVILTTHILEVAERMADRIGIIAGGKLLAEGRL
                     DELRAQAGRAGGSSTLEDVFLQLTAESASA"
BASE COUNT          202 a          431 c          392 g          149 t
ORIGIN      
        1 atcagcacgc caaacacccc cagccacccc ggatggccat acaggatcga cggcaacaga
       61 agcggggcgg cgaagaccat gaagatggtc gcgaccccgg tggcgacgcc cagcgagcgc
      121 accagcagca ccttttccgg cgcgatcggc gaggagaaca gcaaatccag atcgccgcgc
      181 tcatacagcg cctcggccga cgcccccagg gtctgggaca gcatcagggt caggacgatc
      241 gcacacaaca ggtcggcgat cagcacggcc agcggatgga tcggcaccgc gtggccatgc
      301 acggccaggg ccagcagcac cccgcccagg caggcgaagg cgaacaggat ccccaggccg
      361 atcagggcgc cgcccccctt gcccgagcgg ctgccgcgat agccgcgcca agccagccgc
      421 atcacgcgga cgcgctctcg gcggtcagtt gcaggaacac gtcctccaga gtggaactgc
      481 cgcccgcccg gccggcctgg gcgcgcagct cgtccagccg gccctcggcc agcaacttgc
      541 cgccggcgat gatgccgatg cggtcggcca tccgctcggc cacttcgaga atgtgggtgg
      601 tcaggatcac cgtgcccccg gcgtcgaccc gggcctgcag catgtctttc accagccgcg
      661 cggcggcggc gtccaggccc gtcaggggct cgtccaggat cagcaggcgc ggatcgtgga
      721 tcagggcgcc ggccagggcg accttctgct tcatgccgcg cgaaaagccc tcgcagcggc
      781 ggcgggcctc gtcggccagg cccagcttgg ccagcagctc ttcggcccgg gtcctggcca
      841 gggcaggatc gacgttccag aggccggcga cgaactccag atattccagc ggcgtcaacc
      901 ggtcatagat catcgcctcg tcgggcgccc aggccaccag ggccttggcg ccggccgggt
      961 cctggcgggc gtcgacgcca tagacgctga tctcgcccgt gtcgggcctg gtcaggccgg
     1021 ccaccatccg cagcgtcgtc gtcttgcccg cgccattggg ccccagcagg gcatacagct
     1081 cgcccgcatg gacggtcagg tccagatcga cgacagcggg cttggcgaag gtcttgttca
     1141 gaccccgcac gatcaaggct tcggtcaacg ctgc
//
LOCUS       DAOMEO010001164         1174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12063_length_1174_cov_2.186774, whole genome shotgun sequence.
ACCESSION   DAOMEO010001164 DAOMEO010000000
VERSION     DAOMEO010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_1174_cov_2.186774"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLH31_19380"
     CDS             complement(<1..574)
                     /locus_tag="PLH31_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR91390.1"
                     /translation="MKLYDSRRAPNPRRVRWFMAEKGIDDVEIVVLSIMGGDHKSPDY
                     RAKVGISHMPALELDDGTVITESVAICRYLESVYPKPNMFGRDPKETAVIEMWQRRAE
                     MMIVTPLALAVRHTHPRMAALEAQVPEVGENNLVGARRALKMLDRRLAESPFIAADRV
                     TIADVVTVTGVDFGRMIDFRPDAELVHVNRW"
     gene            complement(642..1174)
                     /locus_tag="PLH31_19385"
                     /pseudo
     CDS             complement(642..1174)
                     /locus_tag="PLH31_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517544.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
BASE COUNT          213 a          385 c          382 g          194 t
ORIGIN      
        1 gccagcgatt gacgtgaacc agctccgcgt cgggccggaa atcgatcatt cggccaaaat
       61 cgacgccggt taccgtcacg acgtcggcga tggtcacgcg atcggccgcg ataaacgggc
      121 tctcagccag ccgacgatcc agcatcttca aagcgcgccg ggcgccgacc aggttattct
      181 cgccaacctc ggggacctgg gcctccagcg ccgccatgcg tggatgggta tggcgcacgg
      241 ccagagccaa gggcgtgacg atcatcatct cggcgcgccg ctgccacatc tcgatgactg
      301 cagtttcctt cggatcgcgc ccgaacatgt ttggcttagg gtagacgctc tccagatatc
      361 ggcagatcgc cacggactcg gtaatgacgg tcccgtcgtc gagctccagg gccggcatgt
      421 gcgagatacc gaccttcgcc ctgtagtcgg gcgacttgtg atcaccgccc atgatcgaca
      481 ggaccacgat ctcgacgtcg tcgatgccct tctcggccat aaaccagcgc acccggcggg
      541 ggttgggggc gcggcggctg tcatagagct tcatacgaaa atcctcccgg cttttgaccg
      601 ggaggatgct tggttttcgc agcggaagga aaggcccttc cctaaagccc gaacagcgcc
      661 cggggcatca ggccgatcag cgaaccggtc aggcaggtgg ccaggaagcc ggccatcatc
      721 gccttccaga ccaggcccag cacctcggcc cggcgctcgg gcaccaggac gctgaagccg
      781 gcgacgttca tgccgaccga accgatgttg gcgaagccgc acagggcgta ggtcatgatc
      841 atccgggtgc gctcatccag cagggcctcg ccggtctggg ccagctggat gaaggcggtg
      901 aactcggtga ggatcagctt gacgcccagc aggccgcgcg aagaccacgc ccaggccgcg
      961 ggcgatgctc agcggctggc cgccgacatc gggcagcgcg cccaggccct tgtcgaccat
     1021 ggtggccagg gccacgaaga cgatcagggt ggccccgacg ttcagcgcga tctgcagacc
     1081 gtccgtggtg cccttcatca cggcgtcgat cgagctgcca taggtcttgt cgtcggcggc
     1141 gaggtcgagg tcggagctct tttccatcgg gtcg
//
LOCUS       DAOMEO010001165         1173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12078_length_1173_cov_1.945438, whole genome shotgun sequence.
ACCESSION   DAOMEO010001165 DAOMEO010000000
VERSION     DAOMEO010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12078_length_1173_cov_1.945438"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLH31_19390"
     CDS             <1..1117
                     /locus_tag="PLH31_19390"
                     /inference="COORDINATES: protein motif:HMM:NF014722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HQR91391.1"
                     /translation="ATKGSGQTPWSRQGDGRLTLKGGMREILAASLLEALGVPTSRAF
                     SLVETGEDLQRGDEPSPTRSAVLTRLSHSHIRFGTFQRQAYFERPDLITVLVDHAVET
                     YYPHLADAPDRPAVLMRAVVEASARLLAGWMAAGFVHGVLNTDNMVITGESFDYGPWR
                     FLPRNDPNFTAAYFDHSGLYSFGRQPEAVFWNLQQLAACLTLVTDQAGLLDALNGFSD
                     AYRDALRAAMLRRLALKPRDLATDMNLVSAAFKALAAGGDTLRWEPFFFDWFGGSASE
                     GRAMAGPRAALYGEEAFADFRRRLAEFEPDRPERLDHPIFAQPDPEELLIEHVEALWA
                     PIASQDDWSPLHAKLDRIEAARRAYGFGDDGAENNAV"
BASE COUNT          177 a          397 c          390 g          209 t
ORIGIN      
        1 ggccaccaag ggctcgggcc agacgccgtg gtcgcggcag ggcgacgggc ggctgaccct
       61 gaagggcggc atgcgcgaga tcctcgcggc cagtctgctg gaagccctgg gtgttcccac
      121 ctcaagggcg ttttcgctgg tcgagacggg cgaggacctg cagcgcggtg acgagcccag
      181 cccgacgcgg tcagctgtgc tgacccggct gtcgcacagc cacatccgtt tcgggacctt
      241 ccagcgccag gcctatttcg agcggccgga cctgatcacg gtgctcgtcg atcacgcggt
      301 cgagacctat tatccgcatc tggccgacgc gcccgatcgc cccgccgtgc tgatgcgggc
      361 ggtggtggag gccagcgcca gacttctggc cgggtggatg gcggcgggct ttgtccatgg
      421 cgtgctcaac accgacaaca tggtcatcac cggcgagagt ttcgactatg gcccgtggcg
      481 gttcctgccg cgcaatgatc cgaacttcac cgccgcctat ttcgaccata gcggcctcta
      541 cagctttggc cgtcagcccg aggcggtgtt ctggaacctg cagcaactgg ccgcctgcct
      601 gaccctggtc actgaccagg ccgggcttct ggacgcgcta aacggctttt ccgacgccta
      661 tcgcgacgcc ctgcgcgcgg cgatgctgcg gcgcctggcg ctcaagccgc gtgatctcgc
      721 gaccgacatg aatttggtca gcgcggcctt caaggcgctt gcggcggggg gcgacaccct
      781 gcgctgggag cccttcttct tcgactggtt cggcggatcg gcctcggaag gccgcgccat
      841 ggccggcccc cgcgccgccc tctatggcga ggaggccttc gccgatttcc gccgccggct
      901 ggcggagttt gaacccgaca ggccggaacg tctcgaccac ccgatcttcg cgcaaccgga
      961 tccggaggaa cttctcatcg agcatgtcga ggccctctgg gcgcccatcg cgtcccagga
     1021 cgactggagc ccgctccatg ccaagcttga ccggatcgaa gccgcgcggc gggcctatgg
     1081 ctttggcgat gacggcgcgg aaaacaacgc agtctgaact tgtctgtacg ggtccggaac
     1141 aattgtcatg gttcgggctt acggcttctg cag
//
LOCUS       DAOMEO010001166         1172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12086_length_1172_cov_2.676813, whole genome shotgun sequence.
ACCESSION   DAOMEO010001166 DAOMEO010000000
VERSION     DAOMEO010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_1172_cov_2.676813"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLH31_19395"
     CDS             <1..1121
                     /locus_tag="PLH31_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQR91392.1"
                     /translation="ARLTRELDKLKSLGVTNLRVLGSGERSPAKVAMDPTFRGPGEDY
                     SADLLTGLDFLLAEMARRDMKAVIYLNNFWDWSGGMPAYLNWTGNGTWFQQGDPAHPW
                     PEYPDYSARFYGDAKANAMFRHFIRAVVGRTSTISGKPYRDDPTIMAWQLANEPRPGG
                     TEAFGVRNLPTYYRWIDETAGYIKTIDPNHLVSTGSEGAMGCLEREACVVEAHKPASI
                     DYITLHVWPNNWSWIDFKNQSGTYEAGEARCRVTRHIALAKGLNKPLVIEEFGLIRDD
                     RNFTPGAAATYKDRFYTTIFDLTLADMKAGGPVAGTNFWAWNGEGRAQQPDAWFKRGD
                     TSLVGDPPQEEQGLFGVFDADVSTLAVVEAHARAVASL"
BASE COUNT          216 a          396 c          376 g          184 t
ORIGIN      
        1 gcgcccgcct cacgcgcgag ctcgacaagc tcaagagcct gggcgtcacc aatctgcggg
       61 tgctgggctc gggcgagcgc tcgccagcca aggtggcgat ggatcccacg ttccgggggc
      121 cgggcgagga ctacagcgcc gacctgctga cgggtctcga cttcctgctg gccgaaatgg
      181 ccaggcgcga catgaaggcc gtgatctatc tcaacaactt ctgggactgg tcgggcggca
      241 tgccggccta tctgaactgg accggtaacg gcacgtggtt ccagcaaggc gacccggccc
      301 atccgtggcc ggaatatcct gactattcgg cccgcttcta cggcgacgcc aaggccaatg
      361 cgatgttccg ccacttcatt cgcgccgtgg tgggccgcac cagcacgatc tccggcaagc
      421 cctatcgcga cgacccgacg atcatggcct ggcaactggc caacgagccg cgtcctggcg
      481 gcacggaggc cttcggggtc aggaacctgc cgacctatta tcgctggatc gacgagacgg
      541 ccggctacat caagaccatc gatcccaacc acctggtttc aaccggcagc gaaggcgcca
      601 tgggctgcct ggagcgcgag gcctgcgtgg tcgaggcgca caagccggcc tccatcgact
      661 acatcaccct gcacgtctgg cccaacaact ggagctggat cgacttcaag aaccagtcgg
      721 ggacctacga ggccggcgag gccaggtgcc gtgtcacccg ccacatcgcc ctggccaagg
      781 ggctcaacaa gccgctggtg atcgaggagt tcggcctgat ccgcgacgac cgcaacttca
      841 cgcccggcgc tgcggccacc tacaaggacc gtttctacac gacgatcttc gacctgaccc
      901 tggccgacat gaaggccggc ggcccggtgg ccggcaccaa cttctgggcc tggaacggcg
      961 aaggccgcgc ccagcagccg gatgcctggt tcaagcgggg cgacaccagc cttgttggcg
     1021 atccgccgca ggaggaacag ggcctgttcg gggtgttcga cgccgatgtc tcgaccctgg
     1081 ccgtggtcga ggcccacgcc agggcggtgg cctcgctcta gagcattttc cgacgaagtg
     1141 gacgccggtt cgtcgtcaga aaatgcgtta aa
//
LOCUS       DAOMEO010001167         1170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12109_length_1170_cov_3.121973, whole genome shotgun sequence.
ACCESSION   DAOMEO010001167 DAOMEO010000000
VERSION     DAOMEO010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12109_length_1170_cov_3.121973"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1170
                     /locus_tag="PLH31_19400"
     CDS             <1..>1170
                     /locus_tag="PLH31_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQR91393.1"
                     /translation="VTQLEGYILYSRGVALTRLVLVALLVILLALFTPWRMTLLAIVE
                     FALYLALFAAAEIAARDPDPDRAFRRLCWQSDVLMFLLVSNACWLAVQIRLYDGPIAQ
                     VEAGLLAICVLLFAALRVHITPISYVIGVVPPALTLIWTAIDWSSPRLLDHYTLAAAL
                     FVAAVLLVTSRQQASDRTLTQALRDLTRKNVALTQAIEEAKAASRAKTDLLAVASHEI
                     RTPLNAVLGFAQILRDKDLTPEQADLLSGVLEGGEQLTRLLDGILDLTHVENGQARLN
                     PVPVDLRRMLLGVMRVWSVHARGLGVDFRFEDADPALSFVVRADAARIEQTLINLISN
                     AMGAVASGGRVTVRLAGTPRGQDLGVLIEVGDTGPAVPLEERPRLFEAFDQTARGR"
BASE COUNT          159 a          407 c          382 g          222 t
ORIGIN      
        1 gtcacccagc ttgaagggta tattctgtac tctcgcgggg tcgccctgac ccggctggtc
       61 ctcgtggccc tgctggtcat tctgctggcg ctgttcactc cctggcggat gaccctgctg
      121 gcgattgtgg agtttgctct ctacctggcc ctgttcgccg ccgcggagat cgccgcccgc
      181 gatcccgatc ctgaccgcgc cttccggcgc ctgtgctggc agtcggacgt gctgatgttt
      241 ctgctggtca gcaacgcctg ctggctggcg gttcagatcc gcctgtatga cggcccgatt
      301 gcccaggtcg aggcggggtt gctggccatc tgcgtgctgc tgttcgccgc cctacgggtc
      361 cacatcacgc cgatcagcta cgtcatcggc gtcgttccgc cggccttgac cctgatctgg
      421 accgcgatcg actggtccag ccccagactg ctggatcact acaccctggc ggcggcgttg
      481 ttcgtggccg ccgtgttgct ggtcacctcc cgccagcagg ccagcgaccg taccctgacc
      541 caggccctgc gcgacctcac ccgcaagaac gtggccctga cccaggccat cgaggaagcc
      601 aaggccgcca gccgcgccaa gaccgacctt ctggctgtgg ccagtcatga gatccgcacc
      661 ccgctgaacg ccgtgctggg cttcgcccag atcctgcgcg acaaggacct gaccccggaa
      721 caggccgacc tgctgagcgg ggtgctggaa ggcggcgagc aactgacccg gctgctggac
      781 ggcattctcg acctcaccca tgtcgaaaac ggacaggcgc gcctcaatcc cgttccggtg
      841 gatcttcgcc ggatgcttct gggggtgatg cgggtctgga gcgtccatgc gcgcggtctt
      901 ggcgtggact tcaggttcga ggacgccgat cctgcgctct ccttcgtcgt gcgggccgac
      961 gcggctcgca ttgagcagac cctgatcaat ctgatctcca acgccatggg cgcggtcgcg
     1021 tccggcggtc gggtgacggt gcgtctggcc ggcacacccc ggggccagga cctgggcgtc
     1081 ctgatcgagg tgggcgatac tggcccggcc gtgccgctgg aagagcgccc gcggctgttc
     1141 gaggccttcg accagaccgc gcgaggacgt
//
LOCUS       DAOMEO010001168         1170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12115_length_1170_cov_2.319283, whole genome shotgun sequence.
ACCESSION   DAOMEO010001168 DAOMEO010000000
VERSION     DAOMEO010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12115_length_1170_cov_2.319283"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..>1170
                     /gene="mobQ"
                     /locus_tag="PLH31_19405"
     CDS             229..>1170
                     /gene="mobQ"
                     /locus_tag="PLH31_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HQR91394.1"
                     /translation="MAIFHFSVKVISRATGASAVASAAYRSASRLHDERLDRDHDFTG
                     KSGVVHSEVMLPDGAPEHLSDRATLWNAVEAGEKRKDAQLSREVEFAIPREMDQAQGI
                     ELARDFVQREMVDRGMVADLNVHWDIGLDGLAKPHAHVMLTMREVGREVGREAGEIGF
                     GAKVRDWNRTELVEHWREAWADHVNERLAQLDIDARIDHRSLDAQGIDLEPQNKIGPA
                     AGRRADEGLEADRLDEHHEIARANGERIIADPRIALDAITKQQATFTQRDLAMFVHRH
                     SDGKEQFDRAMSAVQASPDLVALGQDGHGHDRFTSREM"
BASE COUNT          208 a          372 c          399 g          191 t
ORIGIN      
        1 cctggccttg tcttcgaggg ttttgagttc ggcgtccaag tctcggggtt tgcgcatcgc
       61 agtctccatg agcgcgggat gccctgagga taaagcagcc gatccgagaa ggcttcaggg
      121 tcgccggaac acactgggac ggcccggtcc ctcccgagat tttaatcttg gagggcgcgc
      181 ttatacgtcg tgccgacgta ggctcgggat agtaagtggt ctgtcgccat ggcgatcttc
      241 cacttctccg tcaaagtcat cagccgcgcc accggggcca gcgccgtggc ctcggccgcc
      301 tatcgctcag cctcgcggct gcacgacgag cggctggatc gggatcacga cttcacgggc
      361 aagagcggcg tcgtccattc cgaggtcatg ttgccggacg gtgcgcccga gcatctgtcg
      421 gaccgcgcga cgctgtggaa cgcggtcgag gcgggcgaaa agcgcaagga cgcccagctc
      481 tcccgcgagg tcgagttcgc catcccccgc gagatggatc aggcccaagg gatcgaactg
      541 gctcgcgatt tcgtccagcg tgagatggtc gatcgcggca tggtcgccga tctcaatgtg
      601 cattgggaca tcggcctcga cggactggcc aagccccatg cccacgtcat gctgacgatg
      661 cgtgaggttg ggcgcgaggt tgggcgcgaa gccggcgaga ttgggttcgg cgccaaggtc
      721 cgcgactgga accgcaccga gctggtcgag cactggcggg aagcctgggc cgaccacgtc
      781 aacgagcgcc tggcgcagct cgacatcgac gcgcggatcg atcaccgcag cctggacgcc
      841 cagggcatcg accttgagcc gcagaacaag atcggcccgg ccgccggccg cagggccgac
      901 gaaggtctgg aggccgatcg cctcgacgaa caccacgaga tcgcgcgggc caatggcgag
      961 cggatcatcg ctgatccgcg catcgctctg gacgcgatca ccaaacagca ggcgaccttc
     1021 acccagcgcg atctggcgat gttcgttcac cgccattcgg atgggaagga gcagttcgac
     1081 cgggccatga gcgcggtgca ggcttcgccc gatctggtcg ccttggggca ggacgggcac
     1141 ggccatgacc ggttcaccag ccgcgagatg
//
LOCUS       DAOMEO010001169         1169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12123_length_1169_cov_9.833034, whole genome shotgun sequence.
ACCESSION   DAOMEO010001169 DAOMEO010000000
VERSION     DAOMEO010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12123_length_1169_cov_9.833034"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..958)
                     /locus_tag="PLH31_19410"
     CDS             complement(17..958)
                     /locus_tag="PLH31_19410"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HQR91395.1"
                     /translation="MQRNTKIAGIDVGKSWLDVAIHGQEAARRFANDETGLTAMIAWL
                     AEQGVVRAGLEATAGYERAARSELEAAGLEVIVHQPLEVRLFARLKRWKAKTDRLDAA
                     LIAAATAQVDTVRAAQDPRLVELAQRLTAYEQITDQAAELKTFMEHVDLPDLVEALGQ
                     QIQSLSRLKAKLAAEILARIKACADLLARYRLLLSLPGVGPIVAASLVIRMPELGAMR
                     RGQPAALLGVAPFDRQSGQWKGQSFIGGGRSRPRRLLYLAAIAAKRCDPGFKAFAQRL
                     LKAGKPPKVAVVAVMRKLIEAANLVLTRQQPWTTQTA"
BASE COUNT          182 a          393 c          387 g          207 t
ORIGIN      
        1 tgagcaacta tgttgtttat gcggtttggg tggtccaggg ctgctgtctg gtcagaacga
       61 ggttggcggc ctcgatgagc tttcgcatga cggcgacgac ggcgaccttt ggcggctttc
      121 cggctttgag cagccgttgg gcgaaggctt tgaagccggg gtcgcaacgc ttggcggcga
      181 tggcagcgag ataaagcagt cggcggggtc ggcttcgacc gccgccgatg aagctttggc
      241 ccttccattg acccgattgc cggtcgaacg gggccacgcc gagcaaggcg gccggttggc
      301 cgcgacgcat ggccccaagc tcaggcatgc ggatcaccag gctggcggcg acgatcggtc
      361 cgacgccagg cagcgacagc agcagacggt agcgcgccag gagatcggcg caggccttga
      421 tgcgagcgag gatctcggcg gccagcttgg ccttgagccg cgacaggctc tggatctgct
      481 ggcccagcgc ctcgaccaga tcaggcagat cgacgtgctc catgaaggtc ttcagctcgg
      541 cggcttggtc agtgatctgc tcgtaggcgg tcaggcgttg ggccaattcg accaggcgcg
      601 ggtcctgggc ggctcggacc gtgtcgacct gggccgtcgc cgccgcgatc agagccgcgt
      661 ccagtcggtc ggtcttggcc ttccagcgct tgagccgcgc gaacagccgc acctccagcg
      721 gctggtggac aatcacttca aggccagcgg cctccagctc agaccgcgcc gcccgctcgt
      781 agccggccgt cgcctccagc cccgcgcgga ccacgccttg ctcggccagc caggcgatca
      841 tcgccgtcag gcctgtttcg tcgttggcga accgacgcgc cgcctcctgg ccgtggatcg
      901 ccacgtccag ccaagacttg cccacatcga tcccggcgat tttcgtgtta cgctgcactc
      961 gttccatcga cccatccttg tggcttcgga cccatccgtc cctgcaacca tccgggtcat
     1021 gaaacgtcgg cggcggccct gctcaccctc agcgcctaac gctcaggggt tatcggcccc
     1081 tccgccaact gcccgcttgg cgttggcgcg ccaagcgggc gtcccggaag actggcgcag
     1141 cttacgacaa atgctcacag acaaggggt
//
LOCUS       DAOMEO010001170         1168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12146_length_1168_cov_1.918239, whole genome shotgun sequence.
ACCESSION   DAOMEO010001170 DAOMEO010000000
VERSION     DAOMEO010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12146_length_1168_cov_1.918239"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..593)
                     /locus_tag="PLH31_19415"
     CDS             complement(348..593)
                     /locus_tag="PLH31_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HQR91396.1"
                     /translation="MTRPAKTALALGKIDLEEKTTDLRLKLKGKLAVDLADYQRAFAQ
                     ANDQSLELDQLVPHILAAFIDADKGFQAWRKAQPASS"
     gene            complement(945..>1168)
                     /locus_tag="PLH31_19420"
     CDS             complement(945..>1168)
                     /locus_tag="PLH31_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HQR91397.1"
                     /translation="LFSVGAAAAQDAGNRSSGALVINSAASGVSNSAQQVGQQITGRD
                     LNRQPTLRVRAGWPVRVLVSKDMILAPYP"
BASE COUNT          199 a          351 c          406 g          212 t
ORIGIN      
        1 ggaccgatct tcttgccgcc attcacaacg gcactgcgat ttccgagagc ctcccgcgcc
       61 aacggggggc tctcgccgtt taggggtctc gtctaggtca cgatgaagtc ctctggggcg
      121 accgagcgcg cacggagggt ccgcgacgcg actcagtcga cgacagcgtt cagtgagtct
      181 gacgattcct aaaggcttgg aattgcaccc ttttgggcaa aaagggccga ttcgggcggt
      241 tgacgtcttt ccggaaacga gtccgccaag ctgcgactca gcgcgctagg gattgtggcg
      301 cccgcacacc ccgaccccac ctgttgagag atgcggcggc agaaccacta tgatgaggcg
      361 ggctgcgcct tgcgccaggc ctggaagcct ttgtccgcgt cgatgaaggc cgcgaggatg
      421 tgagggacca gctggtcgag ctccagcgac tggtcgttcg cctgagcgaa cgcgcgctga
      481 tagtcggcca gatcaacggc gagcttgccc ttcagcttca aacgcagatc ggttgtcttc
      541 tcctcgagat cgatcttgcc cagggcgagg gctgtcttgg cggggcgcgt catgggcgtc
      601 tccttgggtt gcctgcagga agcggtcgat cgctgtgaaa gcgaaatcgg tgagcttgag
      661 attgagggcg tcggtctggc ggcggatccg aacacaaagc ggctttgaca gccggacaga
      721 gacccggtcc ttggcgccgc ggacggcggg aaacgtcacc gcctcaccgc cgtcgagcgc
      781 ttcgaccagc tcgcggaagg cgcgctcata gatcagccgc acacagcccc tgggattctt
      841 gcgggtgttc aggcgctcgg cggtgtcgag catgtcctgg tgaaggcctt ccggaaggaa
      901 gaggccgagc ttttctgccg atggggtcat cgcaacactc acgcttacgg gtacggggcg
      961 aggatcatgt ccttgctgac gagcacgcgg acgggccaac cggcccggac gcgcagggtg
     1021 ggctggcgat tgaggtcgcg cccggtgatc tgctggccaa cctgctgggc ggaattggac
     1081 accccgctgg ctgccgagtt gatgacgagg gcgccggagc tcctgttgcc ggcgtcctgg
     1141 gctgcggcgg ccccgacgct gaacagcg
//
LOCUS       DAOMEO010001171         1167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12156_length_1167_cov_2.604317, whole genome shotgun sequence.
ACCESSION   DAOMEO010001171 DAOMEO010000000
VERSION     DAOMEO010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12156_length_1167_cov_2.604317"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLH31_19425"
     CDS             complement(<1..398)
                     /locus_tag="PLH31_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR91398.1"
                     /translation="MTPPQGASPVGDVSEVPFKPLPMKGPDVSVDYRPDGSLVITSNH
                     APGEGPRSIAHLLAQKAGEHPDRPYLKQREPGHGPWRGVTYGQALRAVEGIAQWLLDQ
                     GLTSDDSVMILSGNSIEHALMTLGAYTAGVP"
BASE COUNT          184 a          372 c          407 g          204 t
ORIGIN      
        1 ggaaccccgg ccgtataggc ccccagggtc atcagggcgt gctcgatgga attgcccgac
       61 aggatcatca cgctgtcgtc ggacgtcagg ccctggtcca gcagccactg ggctatgcct
      121 tcgacggcgc gcagggcctg gccgtaggtg acgccgcgcc acggcccgtg gcccggctcg
      181 cgctgcttca gatagggccg gtcgggatgc tcgccagcct tctgggccag caggtgggcg
      241 atagagcgcg gcccctcgcc gggcgcgtgg ttggaggtga tgaccagcga accgtcgggc
      301 cgatagtcga cactgacgtc cggtcccttc atcggcagcg gcttgaaggg aacctcgctg
      361 acgtcgccca cgggcgacgc gccttggggc ggcgtcatgg tttctctccc gggtaggtta
      421 tcgttgttct gttggatcgt gacgttaacg gaaccggggc ggacggcaag gatgacgctg
      481 ggggagggct gggcgcacgg cttcgccccc tcgcgcggaa accgccgagt ggatgggaag
      541 ggctgcggag cgtccgggcc tcgcccggat gagtgtgtag tgaataccgt ttcgacgaag
      601 acgaacgctc cgcgcggtct gcttccagaa gccttcgagg cgcggggttt taacccgctc
      661 tcgatattcg gcctccggcg ggcggagggg ctggctagcc ccgtccggac cgcatgagcg
      721 atttcagccc atgcctgctg cgtgccgccg gaccatcgcc gtgggattga tcctgaaccc
      781 cgatccgaag cgcccgcctt ccctggaggg acaggccacg cctcgcaaac cgggcgaaca
      841 gggcccagga cgacagccga cgggcgcttc ccgctcgacc acccctgaca gccgctacgg
      901 tcgccggcct agcgcccttc ccgtgctgtc aggtgcaagc agtatgcggg cggttttgga
      961 gcgggggata caaaagggcg agaaagttgg aagtgattga tttcactgac ttcgattctc
     1021 tattcgtaga ggataggtcg cgaaaacaag tcctccccct agcgcgggag gtgtcgcccc
     1081 tgacttgatc cggggcgacg gaggggaagt gctgctgagt cattgcggtc ttcccccacc
     1141 ggcctccggc cgcctccccc ttcaggg
//
LOCUS       DAOMEO010001172         1166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12179_length_1166_cov_2.055806, whole genome shotgun sequence.
ACCESSION   DAOMEO010001172 DAOMEO010000000
VERSION     DAOMEO010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12179_length_1166_cov_2.055806"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="PLH31_19430"
     CDS             complement(<1..97)
                     /locus_tag="PLH31_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91399.1"
                     /translation="MDFNDSLEEAAYREKARAWLAQNAAEHRAQFG"
     gene            complement(110..>1166)
                     /locus_tag="PLH31_19435"
     CDS             complement(110..>1166)
                     /locus_tag="PLH31_19435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91400.1"
                     /translation="DGRCGPAVVRGVLDDPARSFDQDLWAAVAEQGWLGACIPEEFNG
                     LGLGRVELCAIAEELGRAVAPIPFASTVYFLAEGVMLAGSEAQKAAVLPRIAAGELIG
                     CLATSEGPGVVNASNLACRVEGGKLTGVKIPVTDGDVADMALVLASEGGKAGLFLVDL
                     KSKGVARETLKSLDPTRGVAKLTFDGVTAERVGEAGAGFDLVQAIMDRAAVLLAFEQL
                     GGSDKCLEMAKDYALGRYAFGRVIAGYQAIKHKLADMYVKNEVARSNAYYGAWALNVD
                     APELPIAASAARIAASDAYWFASKESIQVHGGMGFTWDVDCHLYYRRSRQLSLVAGAP
                     RVWKERLVSQLEQKNAA"
BASE COUNT          186 a          414 c          382 g          184 t
ORIGIN      
        1 cgccgaactg cgcgcggtgc tcggcggcgt tctgcgccag ccaggcgcga gccttctcgc
       61 gataggcggc ttcttcgagg gaatcgttga agtccatgat tgtctctccc tatgccgcgt
      121 ttttctgttc gagctggctg accagccgtt ctttccagac ccgcggcgcg ccggccacga
      181 ggctgagctg gcgcgagcgc cggtaataga ggtggcagtc gacatcccag gtaaagccca
      241 tgccgccatg gacctggatg ctttccttgc tggcgaacca gtaggcgtcg gacgccgcga
      301 tgcgcgcggc cgaggcggcg atcggcagtt ccggcgcgtc cacgttcagg gcccaggccc
      361 cgtaataggc gttggagcga gcgacctcgt tcttgacgta catgtcggcc agcttgtgct
      421 tgatcgcctg atagccggcg atgacgcggc caaaggcgta gcggcccagg gcatagtcct
      481 tggccatctc caggcacttg tcggacccgc ccagctgttc gaaggccagc agcacggcgg
      541 cgcgatccat aatcgcctgg acgaggtcga aaccagcgcc cgcctcgccc acccgctcgg
      601 cggtcacgcc gtcgaaggtc agcttggcca cgccgcgcgt gggatccagg ctcttgagcg
      661 tctcgcgcgc cacgcccttg gacttcagat cgaccaggaa cagacccgcc ttgccaccct
      721 cgctggccag gaccagggcc atgtcggcca cgtcgccgtc agtgaccggg atcttcacgc
      781 ccgtcagctt gccgccttcg acccggcagg ccaggttcga ggcattgacc acgcccggac
      841 cttccgaggt cgccaggcag ccaatcagtt caccggcggc gatgcgcggc aggacggcgg
      901 ccttctgggc ctcgctgccg gccagcatca caccctcggc caggaaatag accgtcgagg
      961 cgaacgggat cggcgccacg gcgcggccca gttcctcggc gatggcgcac agctccaccc
     1021 ggccaaggcc caggccgttg aactcttcgg ggatgcaggc gcccagccaa ccctgctcgg
     1081 cgacggcggc ccacaggtcc tgatcgaagg agcgggccgg gtcatcgaga acgccgcgca
     1141 ccacggccgg accgcagcgt ccgtcg
//
LOCUS       DAOMEO010001173         1165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12188_length_1165_cov_2.892793, whole genome shotgun sequence.
ACCESSION   DAOMEO010001173 DAOMEO010000000
VERSION     DAOMEO010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12188_length_1165_cov_2.892793"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..>826)
                     /locus_tag="PLH31_19440"
     CDS             complement(163..>826)
                     /locus_tag="PLH31_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQR91401.1"
                     /translation="AVYGGPHAQNVRKIWQSPSERTYLEAGYLVFRLDNRGTANRSAK
                     FARALDRRLGTVEVDDQLVGANYLKSLPYVDAEKLGVVGWSYGGFMTLMMLTAENTPF
                     KAGAGGAPPTEWGLYDTAYTERYMGKPDENKDGYARSDILGRLDRMKPGSLLLLHGMA
                     DDNVIFENSTRLIAELQKKAIPFEMALYPGERHSAPGSKTKGLSVLKTHLEFFGRKLK
                     GQ"
     assembly_gap    827..836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<837..>1165)
                     /locus_tag="PLH31_19445"
     CDS             complement(<837..>1165)
                     /locus_tag="PLH31_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQR91402.1"
                     /translation="YEVSYAKPGKPKALTPAGGWWTAKVAPNGAFAGTYNDPKTPPQT
                     ALYAPDGKRVRWIEENRLAEGHPYWPYASTLTTPEYGTLKAADGEALHYALLKPADFD
                     PSKKYPA"
BASE COUNT          168 a          352 c          399 g          236 t
ORIGIN      
        1 cttcatcagt gatcttggtc atggcgctct cctcacatcc gcgctgagga gaatagaaac
       61 tgctttgcgt cttggggctg tgacaaaagg tcggcccggg cgcgcctttt gacgcagcct
      121 ccgttcgcgg cggggtcgcc gcgaacggat cagttgaagg gtctactggc ccttcagctt
      181 gcggccaaag aactccagat gggtcttcag cacgctgaga cccttggtct tgctgccagg
      241 cgcgctgtgg cgctcgccgg gatagagggc catttcgaag gggatggcct tcttctgcag
      301 ctctgcgatc aggcgtgtgc tgttttcgaa gatgacattg tcgtcggcca tgccgtgcag
      361 cagcagcagg ctgccgggct tcatcctgtc caggcgaccc aggatgtcgg accgcgcata
      421 gccgtccttg ttctcgtccg gcttgcccat gtagcgctcg gtataggcgg tgtcgtacag
      481 gccccattcg gtgggcggcg cgccgccggc gccggccttg aacggggtgt tttcggcggt
      541 cagcatcatc agggtcatga agccgccata cgaccagccg accacgccca gcttctcggc
      601 gtccacatag ggcaggcttt tcagatagtt ggcgccgacc agctggtcgt cgacctccac
      661 cgtgcctagg cggcgatcca gggcccgcgc gaacttggcc gagcggttgg cggtgccgcg
      721 gttgtcgagc cggaagacca gatagccggc ttccagatag gtgcgctccg acgggctctg
      781 ccagatcttc ctgacgttct gggcgtgcgg gccgccatag acggcgnnnn nnnnnnatgg
      841 ccggatactt tttcgacggg tcgaaatcgg ccggcttcag cagggcgtag tgcagggcct
      901 cgccgtcggc ggccttcagg gttccgtatt ccggcgtggt cagggtggag gcgtacggcc
      961 agtagggatg accctcggcc agccggttct cctcgatcca gcgcacgcgc ttgccgtcgg
     1021 gcgcgtagag ggcggtctgg ggtggagtct tggggtcatt gtaggtgcca gcaaaggcgc
     1081 cgttgggggc caccttggcc gtccaccagc cgccggccgg cgtcagggcc ttgggcttgc
     1141 ccggtttggc gtaggacacc tcata
//
LOCUS       DAOMEO010001174         1163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12237_length_1163_cov_2.555054, whole genome shotgun sequence.
ACCESSION   DAOMEO010001174 DAOMEO010000000
VERSION     DAOMEO010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_1163_cov_2.555054"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLH31_19450"
     CDS             complement(<1..843)
                     /locus_tag="PLH31_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR91403.1"
                     /translation="MSAPSHPELKAPAGSAGLLALSIISALVAIVAAGLSQDPIFRFQ
                     VEIFALGAIVAAFALNAGLASGSLREDPDRYSDNVIKAGVIATMFWCLAGLAAGTLIA
                     AQLSWPRIFYFPELGFLNFGRLRPLHTSAVIFAFGGNALIATSFWVVQRTCKAKLAGG
                     IAPWFVFWGYQLFIVLAATGYLAGATQSKEYAEPEWLTDIWLTIVWVAYLLVFLGTIW
                     KRKEPHIYVANWFYLAFIVTVALLHLVNNAAVPVSPLSHKSYGVFAGVQDALVQWWYG
                     HNAVG"
     gene            966..>1163
                     /locus_tag="PLH31_19455"
     CDS             966..>1163
                     /locus_tag="PLH31_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91404.1"
                     /translation="MSPVSVRAWRNLQKLLGLTTVISWALALWQAAPRLMAGPLCTAA
                     QDPLGLAGPHCPACVVAVLSTL"
BASE COUNT          223 a          373 c          381 g          186 t
ORIGIN      
        1 gccgacggcg ttgtggccgt accaccactg gaccagggcg tcctgaacgc cggcgaagac
       61 gccataggac ttgtggctca gcgggctgac cggcaccgca gcgttgttca ccaggtgcag
      121 cagggccacg gtgacgatga aggccagata gaaccagttg gccacataga tatgcggctc
      181 cttgcgcttc cagatcgtgc ccaggaagac cagcagatag gcgacccaga cgatggtcag
      241 ccagatgtcg gtcagccatt cgggctcggc gtactccttg ctctgggtgg cgccggccag
      301 atagccggtc gccgccagca cgatgaacag ctgatagccc cagaacacga accacggcgc
      361 gataccgccc gccagcttgg ccttgcaggt gcgctgaacc acccagaagg acgtcgcgat
      421 cagggcgttg ccgccgaatg cgaagatcac cgcagaggtg tgcagcgggc gcaggcgtcc
      481 aaagttcaga aagcccagtt cgggaaagta gaagatccgg ggccaggaca gctgggccgc
      541 gataagcgtc ccggccgcca gaccggccaa gcaccagaac atggtggcga tcacgccggc
      601 cttgatgacg ttgtcgctat aacggtcagg gtcttcgcgc agacttcccg acgccaggcc
      661 tgcgttcagg gcgaaggcgg cgacgatcgc gcccagggcg aagatctcga cctgaaacct
      721 gaagatcgga tcctggctca agcctgcggc gacaatcgcg acaagggcgc tgatgatcga
      781 cagggcgagc aatccagcgg agcccgccgg cgctttcaat tcagggtggg acggggcgga
      841 catcgagact ccgttcagcg aacttgcccc tgcctctcac gatcccgtcg cgaccacttt
      901 gatccttgtc aaagccaccc cggttgcagg gggcgatgat gacggtcgct actctggagg
      961 tcctgatgtc gcctgtttct gtccgcgcct ggcgcaacct ccaaaagctt ctgggcctga
     1021 cgaccgtgat cagctgggcg ctggccctgt ggcaagcggc gccgcgcctg atggcgggac
     1081 cgctttgcac tgcggcccag gatcccctgg gactggcggg gccgcactgc cccgcttgcg
     1141 tcgtggccgt tctgagcacc ctg
//
LOCUS       DAOMEO010001175         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12254_length_1162_cov_2.851852, whole genome shotgun sequence.
ACCESSION   DAOMEO010001175 DAOMEO010000000
VERSION     DAOMEO010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12254_length_1162_cov_2.851852"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLH31_19460"
     CDS             <1..556
                     /locus_tag="PLH31_19460"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQR91405.1"
                     /translation="APPMNGRRIVASPFAGTVIQVNVLEGQSVKAGAALAVLFSRDAL
                     TVGSELSQARAELGVAEAAARRSRTLAAEGIIAGARVEEANARAAQARARVSERQRLI
                     SSAGGAGARPGEFILRAPISGRIAQLNIAPGAGLEAMAPALTIDRSDKLWLEARLSPD
                     LVDRVKVGGAARVGGVTGRIIAVG"
     gene            560..871
                     /locus_tag="PLH31_19465"
     CDS             560..871
                     /locus_tag="PLH31_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91406.1"
                     /translation="MDPRTRSVVVRAEIAGDGRLAPGQTASLTLLGPVQPGAVQIPRS
                     AVIQGARGAMVFVREAKGYRSAPVTVIGAAGQFAVITGLAPGARVAVSGVSQLKSALD
                     Q"
     gene            874..>1162
                     /locus_tag="PLH31_19470"
     CDS             874..>1162
                     /locus_tag="PLH31_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR91407.1"
                     /translation="MFQKLISFALSQRLLAIVLTVALVGLGLNAYLKLPVDAFPDISP
                     TQVKIILKAPGMTPEEVESQVITPLEMELLGIPRQSMLRATAKYAIADLTID"
BASE COUNT          167 a          392 c          409 g          194 t
ORIGIN      
        1 tgcgcctccg atgaatggcc gccggatcgt ggcctcgccc ttcgctggca cggtgatcca
       61 ggtcaatgtg ctggaggggc aaagcgtcaa ggcgggcgcc gctctggctg tcctgttcag
      121 ccgcgacgcg ctcaccgtgg ggtcggagct cagtcaagcc cgggccgagc tcggcgtggc
      181 cgaggcggcg gcgcggcgct ccagaaccct ggcggccgaa gggattatcg ccggagcgcg
      241 ggtcgaagaa gccaatgccc gcgccgccca ggcaagggcc agggtctcgg aacgtcaacg
      301 cctgatcagc agcgccggtg gggccggagc ccgaccgggc gagttcattc tgcgcgctcc
      361 gatcagcgga cgcatcgccc agctgaacat cgcgcccggc gccgggcttg aggccatggc
      421 gcccgccctg accatcgacc gcagcgacaa gctgtggctg gaggcccggc tgtctcccga
      481 ccttgtcgat cgcgtgaagg ttggcggcgc ggcgcgggtc ggcggcgtga cgggacgcat
      541 catcgcggtc ggatagtcgg tggatccgcg cacccgttcc gttgtcgttc gggccgagat
      601 cgccggcgat ggccggctgg cgccgggaca gacggccagt ctgaccttgc tggggcctgt
      661 tcaacccggc gctgtccaga tcccgcgttc ggcggtcatc cagggcgcgc gtggcgcgat
      721 ggtcttcgtc cgggaggcca agggctatcg cagcgccccc gtcaccgtca tcggcgcggc
      781 ggggcagttc gccgtcatca ccggtctggc tcccggtgcg cgcgtggccg tcagcggtgt
      841 ctcgcaactc aagtcggctt tggaccagta gtgatgttcc agaagctgat ttcctttgcc
      901 ttgtcgcagc ggctgctggc gatcgtcctg accgtggcct tggtgggcct tgggctcaac
      961 gcctatctca agctgccggt cgacgccttc ccagacatct cgcccaccca ggtgaagatc
     1021 atcctcaagg cgcccggcat gacgcccgaa gaggtcgaga gccaggtcat cacgccgctc
     1081 gagatggagc tgctgggcat tccgcgtcag agcatgctgc gggccacggc caagtacgcc
     1141 atcgccgacc tgaccatcga tt
//
LOCUS       DAOMEO010001176         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12261_length_1162_cov_1.948509, whole genome shotgun sequence.
ACCESSION   DAOMEO010001176 DAOMEO010000000
VERSION     DAOMEO010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_1162_cov_1.948509"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLH31_19475"
     CDS             <1..369
                     /locus_tag="PLH31_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HQR91408.1"
                     /translation="DALRLAALAPEMMISVPVSSTEHLATLVKGGLEARRILAWTGTR
                     AENPALWASLREAGVEPMFGTLGAPGRRADDRYAADGDPSEYRGLAKAGVAVIGTDAP
                     KVVRAMLAEVAGAPSTCKLP"
     gene            492..986
                     /locus_tag="PLH31_19480"
     CDS             492..986
                     /locus_tag="PLH31_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91409.1"
                     /translation="MRRSFVIIALGAVLGGPAFAAPKVATVRVSIGQELARKTQIMDA
                     REFDYLSRELKRSVETRLDRSNALDPEGGELDLVIEDARPNRPTWREMSAKPGLSYQS
                     FGVGGARVSGEYPSASGGRTPVAYSWYETDIRWAQYGSTWSDADQAFDRLAARLAKNQ
                     FSDR"
     gene            1068..>1162
                     /locus_tag="PLH31_19485"
     CDS             1068..>1162
                     /locus_tag="PLH31_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91410.1"
                     /translation="MTDRRSSWSLAAFAAPCLPLAALGLPLVVYLP"
BASE COUNT          203 a          369 c          383 g          207 t
ORIGIN      
        1 gacgccctgc gtctggccgc cttggcgccc gaaatgatga tctccgtacc agtgtccagc
       61 accgaacatc tggcgaccct ggtcaaggga gggctggaag cccgccgcat cctggcctgg
      121 accgggacac gggcagagaa cccggcgctt tgggcgagcc ttcgcgaggc cggcgtcgag
      181 cccatgttcg gaacccttgg cgcgcccggg cgtcgggcgg acgaccgcta tgcggccgat
      241 ggtgacccaa gcgaatatcg gggcctggcc aaggcgggag tcgctgtgat tggcacggat
      301 gcgcccaagg tcgtcagggc gatgctcgcc gaggtcgctg gcgccccctc gacctgcaag
      361 cttccctgag cctgctcgcg gttaccgcag aacctgcgaa caaacgtttg gaatgctccc
      421 gtcggggtct ggcgttttgg tccggttagc cgcacagttt cgaacaatgg atcagatccg
      481 gagttccgac gatgcgacgc tcgtttgtga tcattgcttt gggcgccgta ctcggaggtc
      541 ccgccttcgc cgctccaaag gttgcaacgg tgcgcgtctc gatcggacag gaactggcca
      601 ggaagaccca gatcatggat gcgcgtgagt tcgactatct gagccgcgaa ctcaaacggt
      661 ccgtcgaaac aaggctggat cgttcgaacg cgttggatcc cgagggcggc gaactggatc
      721 tcgtcatcga ggacgcacgg cccaatcgcc cgacgtggcg ggaaatgagc gccaagcctg
      781 gactgtccta ccagagcttc ggcgtcggcg gagcccgcgt cagcggtgaa tatccatccg
      841 cgtcaggcgg ccgcacgcca gtggcctata gctggtacga gacagatatt cgatgggccc
      901 agtacggctc cacctggagc gacgccgacc aggccttcga caggctcgcg gcccgcctgg
      961 ccaagaatca attcagcgat cgctagagca gcgttccagg gcggccgcgc ccagtgagtt
     1021 caagaggcgg gagccaaccc gtcgtgtttg ttaggggact acgcgcaatg accgatcgac
     1081 gatcgtcctg gtctctggcg gccttcgccg caccctgcct gccgctcgcg gcgctgggtt
     1141 tgccattggt cgtctatctg cc
//
LOCUS       DAOMEO010001177         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12285_length_1160_cov_3.709502, whole genome shotgun sequence.
ACCESSION   DAOMEO010001177 DAOMEO010000000
VERSION     DAOMEO010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12285_length_1160_cov_3.709502"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PLH31_19490"
     CDS             <1..1160
                     /locus_tag="PLH31_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HQR91411.1"
                     /translation="QDKSFFFLEMNTRLQVEHPVTELITGLDLVEQMIRSAWGEKLAF
                     EQKDLKINGWAIESRIYAEDPYRKFLPSIGRLVRYAPPAEGQHDGYTVRNDAGVREGD
                     EISMFYDPMISKLCTWAPTRIAAVDGMGRALEDFHIEGLGQNIPFLNAVMDQERFRSG
                     QLSTNYIKDEFPDGFGGTEPTAAQIDILTVVGAAMQRVYAARALTNVTGLNGPVRDEW
                     VVAVGHAKRRVKLSGDENLTVELLDEGRTLSLGEVDWRPGKPVFRGALGGAAFTVQVA
                     PAAEGFTIRHRAAKARVLVLTPRSADLHDKLPEKQAADTSRLVLSPMPGLVVSMDVAA
                     GQQVREGEVVCVLEAMKMQNIIRAERDGVVKAVNAKGGDPVAADEVLVEFA"
BASE COUNT          198 a          382 c          404 g          176 t
ORIGIN      
        1 gccaggacaa gagcttcttc ttcctggaga tgaacacccg cctgcaggtc gagcacccgg
       61 tcaccgagct gatcacgggg ctggacctgg tcgagcagat gatccgctcg gcctgggggg
      121 aaaagctggc cttcgagcag aaggacctga agatcaacgg ctgggccatc gagagccgca
      181 tctatgccga ggatccctac cgcaagttcc tgcccagcat cggccggctg gtgcgctacg
      241 ccccgccggc ggagggccag cacgacggtt atacggttcg caacgacgcc ggggtgcgcg
      301 aaggcgacga gatctcgatg ttctacgacc cgatgatctc caagctctgc acctgggccc
      361 cgacccgcat cgccgccgtc gacggcatgg gccgggcgct ggaagacttc cacatcgagg
      421 gtctgggtca gaacatcccg ttcctcaatg cggtgatgga ccaggagcgg ttccgctcgg
      481 gccagctgtc gaccaactac atcaaggacg agttccccga cggctttggc ggaaccgagc
      541 ccacagccgc ccagatcgac atcctcaccg tggtcggcgc ggccatgcag cgggtctatg
      601 ccgcccgggc gctgaccaat gtcaccggcc tcaacggccc ggtgcgcgat gaatgggtgg
      661 tggcggtcgg ccacgccaag cgccgggtca agctgtcggg cgatgagaac ctgacggtcg
      721 agctgctcga cgagggccgc acgctgagcc tcggtgaggt cgactggcgt ccgggcaagc
      781 cggtgttccg gggcgctctg ggcggagcgg ccttcaccgt tcaggtggct ccggcggccg
      841 agggcttcac catccgccac cgcgcggcca aggcccgggt gctggtgctg accccccgct
      901 cggccgatct gcacgacaag ctgcccgaga agcaggccgc cgacacctcc aggctggtgc
      961 tctcgccgat gccggggctg gtggtctcga tggacgtcgc cgccggccag caggttcgcg
     1021 aaggagaggt ggtctgtgtg ctggaggcca tgaagatgca gaacatcatc cgcgccgagc
     1081 gcgacggcgt ggtcaaggcc gtcaacgcca agggcggcga ccccgtcgcg gccgatgaag
     1141 tgctggtgga gttcgcctga
//
LOCUS       DAOMEO010001178         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12286_length_1160_cov_2.936652, whole genome shotgun sequence.
ACCESSION   DAOMEO010001178 DAOMEO010000000
VERSION     DAOMEO010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12286_length_1160_cov_2.936652"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLH31_19495"
     CDS             complement(<1..1137)
                     /locus_tag="PLH31_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQR91412.1"
                     /translation="MSAPQSARPVPPVAKKEPKRIEQLGRVRVDDYAWMKDDNWQKVL
                     RDPSLIKADVKEHLTAENAYTKAMLASTEPLQTAMFEEMKGRIKQDDASVPAPDGAWE
                     YYTRFEIGAQHPIHARKPRAGGPEQVLLNEETESKGKAFYQVGAAGHSPDHKLYAFAV
                     DEQGSEVYRIHVKDLATGAVLESPVESTTGDFCFSPDSQWLFWTFRDDNGRPARIYRR
                     PARGGAKDDVLIYDEPDDGFFIGVGTVSSEKFIVISCGNQETSEALLIPASDPTAKPV
                     VVEPRTVGLRYELDHWNDHFVIRTNADGAVDWKLVTAPEATPGKAHWKDWVAHTPGRL
                     IMGMTAFKNHFARLEKVDAVNRIVITAAGGEEHVELVHGEVPEAE"
BASE COUNT          175 a          366 c          394 g          225 t
ORIGIN      
        1 ttccgcttct ggaacctcgc cgtggacgag ctcgacgtgc tcctcgccac ccgctgcggt
       61 gataacgata cgattgacgg cgtccacctt ctccagccgc gcgaagtggt tcttgaacgc
      121 agtcatgccc atgatcaggc ggccgggggt gtgggccacc cagtccttcc agtgcgcctt
      181 gccgggcgtg gcctcgggcg cggtgaccag cttccagtcg acggcgccgt cggcgttggt
      241 gcggatcacg aagtggtcgt tccagtggtc cagctcgtag cgcaaaccca ccgtgcgggg
      301 ctcgacgacg acaggcttgg cggtcgggtc gctggccggg atcagcaggg cctcgctggt
      361 ttcctggttg ccgcagctga tgacgatgaa cttctcggac gacaccgtac cgacgccgat
      421 gaagaagcca tcgtccggct cgtcatagat cagcacgtcg tccttggcgc cgccgcgcgc
      481 ggggcggcga tagatcctgg ccgggcggcc gttgtcatcc cggaaggtcc agaacagcca
      541 ctggctgtcg ggcgagaagc agaagtcgcc ggtggtgctc tcgaccgggc tttccagcac
      601 agcgccggtg gccagatcct tgacgtggat gcgataaacc tccgagccct gctcatcgac
      661 agcgaaggca tagagcttgt ggtcgggcga atggccggcg gcgccgacct ggtagaaggc
      721 cttgcccttg ctctcggtct cttcgttcag cagaacctgc tcaggcccgc cggcgcgcgg
      781 cttgcgcgca tggatcggat gctgcgcgcc gatctcgaag cgggtgtaat attcccaggc
      841 gccgtcaggg gccgggacgc tggcgtcgtc ctgcttgatg cggcccttca tctcctcgaa
      901 catcgccgtc tgcagcggct ccgtcgaggc cagcatggcc ttggtgtagg cgttctctgc
      961 ggtcaggtgt tccttgacgt cggccttgat cagcgagggg tcgcgcagca ccttctgcca
     1021 gttgtcgtcc ttcatccacg catagtcgtc gacccgaaca cggcccagct gctcgatgcg
     1081 cttgggttcc ttcttggcga cgggcgggac ggggcgagca gattggggtg cggacatgga
     1141 gattccggga atgaaaacgg
//
LOCUS       DAOMEO010001179         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12290_length_1160_cov_2.204525, whole genome shotgun sequence.
ACCESSION   DAOMEO010001179 DAOMEO010000000
VERSION     DAOMEO010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_1160_cov_2.204525"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1160)
                     /locus_tag="PLH31_19500"
     CDS             complement(<1..>1160)
                     /locus_tag="PLH31_19500"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQR91413.1"
                     /translation="DCASHFVITADEGRRGGKRVPLKANIDEALESCPWVSKVLMVRW
                     TGADVPLVKNRDIVWQDVREAVSAECAPEPMNAEDPLFILYTSGSTGKPKGVLHTTGG
                     YLAWASWTFWAVFDYKPGEVFWCTADVGWVTGHSYVVYGPLANGGTSLIFEGVPNYPT
                     PSRFWEVIDKHRVEIFYTAPTALRALMREGDEHLTKNDLSSLRLLGSVGEPINPEAWL
                     WYHRVVGKEKLPIVDTWWQTETGGMLITPLPGATPLKPGSASKPLPGVKPQLVDAEGG
                     VLDGATDGNLVITDSWPGQMRTVYGDHGRFFETYFSTYPGKYFTGDGCRRDADGYYWI
                     TGRVDDVINVSGHRLGTAEIESALVAHETVAEAAVVGYPHDIKGQGVYAYVTL"
BASE COUNT          184 a          389 c          393 g          194 t
ORIGIN      
        1 agggtcacat aggcatagac gccctggccc ttgatgtcgt gcggatagcc gaccacggcg
       61 gcctcggcga cggtctcgtg agcgaccagg gcgctctcga tctcggccgt gcccagccgg
      121 tggcccgaga cgttgatcac gtcatccacg cggccggtga tccagtagta gccgtcggcg
      181 tcgcgccggc aaccgtcgcc ggtgaagtac ttgccaggat aggtcgagaa ataggtctcg
      241 aagaaccggc catggtcgcc atagactgtg cgcatctggc cgggccagct gtcggtgatg
      301 accagattgc cgtcggtcgc gccgtccagc acgccgcctt cggcgtcgac cagctgcggc
      361 ttgaccccgg gcaggggctt ggaggccgag ccgggcttca ggggcgtcgc gccgggcaga
      421 ggcgtgatca gcatgccgcc ggtctcggtc tgccaccagg tgtcgacgat gggcagcttc
      481 tccttgccga ccacgcggtg gtaccagagc caggcctcgg ggttgatcgg ctcgccgacg
      541 ctgcccagca ggcgcaggga tgacaggtcg ttcttggtga ggtgctcgtc gccctcgcgc
      601 atcagcgccc gtagcgccgt gggggcggtg tagaagatct cgaccctgtg cttgtcgatc
      661 acctcccaga accggctggg ggtgggatag tttggcacgc cctcgaagat caggctggtc
      721 ccgccattgg ccagcgggcc gtagaccaca tagctgtggc cagtcaccca gcccacatcg
      781 gcggtgcacc agaacacttc gcccggcttg tagtcaaaga cagcccagaa cgtccacgaa
      841 gcccaggcca ggtagccgcc cgtggtgtgc agcacgccct tgggcttgcc cgtcgacccc
      901 gaggtgtaaa ggatgaacag cgggtcctcg gcgttcatcg gttcgggcgc gcactcggca
      961 gagacggcct cgcgcacgtc ctgccagacg atgtcgcggt tcttgaccag cggcacgtcg
     1021 gcgccggtcc agcgcaccat cagcaccttg ctcacccagg ggcagctctc cagcgcctcg
     1081 tcgatattgg ccttcagcgg cacgcgcttg ccgccgcgcc ggccctcgtc ggcggtgatc
     1141 acgaaatgcg aggcgcagtc
//
LOCUS       DAOMEO010001180         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12293_length_1160_cov_1.920362, whole genome shotgun sequence.
ACCESSION   DAOMEO010001180 DAOMEO010000000
VERSION     DAOMEO010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_1160_cov_1.920362"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>1160
                     /locus_tag="PLH31_19505"
     CDS             41..>1160
                     /locus_tag="PLH31_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91414.1"
                     /translation="MADIEAAIIRPETPGPVRQAARAVLESAIPTQRPVTSAAMRSAV
                     QGSGVFLEAHLARTAATERGAIATAAIPSAPSDMKAALLVFRAVVSTWLARMPAVESK
                     PQGALSEPSLASRPDPSRVTYTPSSLAHSAADQVDGSADPLRRDAMGANVGPAPPAPP
                     GAPRAPAPGAPVQPSTLGTSASPATASAQALLVTDSPEVPPPPGQDALARPAPAAAPR
                     AAPTLPSQPFLMVGMIEDDAIEPLPLADKEGQEVARDLRSGLPLPPARGPSKPPPPYA
                     GGPTSAQAAVPSDLPQGLTPADLARQLLKGADGAIARQELLQIASLPEPRAEAERTLE
                     TRGSRWVFDLPFHTPQGVAVAHFEISRDGGGGGTEPGRE"
BASE COUNT          167 a          434 c          390 g          169 t
ORIGIN      
        1 ggctcacgcg gcgcccgttc agtctggatt ggcggcggcg atggccgata tcgaggccgc
       61 gatcatcagg cctgaaacgc ccggacctgt gcgtcaggct gcgcgggccg tgctcgaaag
      121 cgccatcccg acccagcgcc ccgtcacctc ggctgcgatg cgctcggctg tgcagggctc
      181 cggcgtcttc ctcgaagccc atctggcgcg caccgcggcg acggaacgcg gagccatcgc
      241 cacggcggcc attccatcag cgcccagcga catgaaggcc gcgctcctgg tctttcgcgc
      301 cgtcgtttcc acatggctgg ccaggatgcc ggcggttgaa tcgaaaccgc agggcgcgct
      361 ctctgagccc tccctggcgt cgagacccga ccccagccgg gtgacctata ccccaagcag
      421 cttggcgcac tcggccgccg atcaggtcga tggctcggcc gatcctctgc gtcgcgacgc
      481 catgggggca aatgttgggc cggcgccgcc tgcgccccct ggcgcccccc gtgcgccagc
      541 tccgggagcg cccgtacagc catcgacctt ggggacatcg gcgtccccgg cgacggcgtc
      601 ggcgcaggcg ctcctggtga cagacagtcc tgaggttcct ccgcccccag gacaggacgc
      661 cttggcaagg cccgcgcccg ccgcagcgcc acgtgcggcg cccaccctgc cgtctcagcc
      721 cttcctgatg gtggggatga tcgaggatga cgcaatcgag cctctgccgc tggccgacaa
      781 ggaagggcag gaggtcgctc gcgatctccg gtccggtctg ccattgccgc cggcgcgtgg
      841 gccttccaag ccgccgccgc cctatgccgg aggaccgaca tcggcgcagg ccgccgtccc
      901 atcagatctc ccccaaggcc tgacgccggc tgacctggcg cggcaattat tgaagggcgc
      961 cgacggcgcc attgcccgcc aggagctcct gcagatcgcc tcgctgccag aaccgcgggc
     1021 cgaagcggag cgaacactcg agacccgagg ctcgcgctgg gtgttcgacc tgccgttcca
     1081 tacccctcag ggcgtcgctg tcgctcactt cgagatcagc cgcgatggcg gcggcggggg
     1141 gacggaaccg ggtcgcgagg
//
LOCUS       DAOMEO010001181         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12315_length_1158_cov_2.271985, whole genome shotgun sequence.
ACCESSION   DAOMEO010001181 DAOMEO010000000
VERSION     DAOMEO010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12315_length_1158_cov_2.271985"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..595
                     /locus_tag="PLH31_19510"
     CDS             158..595
                     /locus_tag="PLH31_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HQR91415.1"
                     /translation="MTVGRRILGLPFLAFGALILSLTATPARADPCEGRLPSRAGEPF
                     SGTVRYVGDGDGLCVGRSGDPDSWIEVRLADFDAPELNASDGRRSKTLLEQVAMGQPV
                     TCEARCGRSGRVIVYDRVIAVCRVRGRAIGDLLRALRAPEGGN"
     gene            complement(819..>1158)
                     /locus_tag="PLH31_19515"
     CDS             complement(819..>1158)
                     /locus_tag="PLH31_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91416.1"
                     /translation="EAQLTTTFLDVVIGRKTFYRAEGGLLVWVLPHFDPAYRQLTVDD
                     LLFNNNADVLILDRESRSLGSGRAVDAALRVPAAGDGGGQPRIGVGERAGRSGRSHDR
                     SGESAYIRLR"
BASE COUNT          193 a          409 c          362 g          194 t
ORIGIN      
        1 agcgcaaggc gcgcgtcgat gatctcgagc ccgaagcgct gatcaccggc cactttaact
       61 tcgacgccga cgagctgagc gtccaccacg cgctcgcccg catcgtcgac atgctggagg
      121 ccgactatgg cctgaaaatt ccacgtgagg agccatgatg accgtgggac gccgcatcct
      181 cggcctgccg ttcctggctt ttggagccct catcctgtct ctgacggcga ccccggcgcg
      241 cgcggacccg tgtgaaggcc gcctcccctc gcgagctggc gagccgttca gcggcacagt
      301 ccgctacgtc ggcgatggcg atggcctgtg cgtcggccgc tccggcgacc ccgacagctg
      361 gatcgaggtg cgcctggccg acttcgacgc gcccgaactc aacgcatcgg acggacgccg
      421 gtcaaagacg cttctggagc aggtcgccat gggacagccc gtgacctgcg aagcgcggtg
      481 cggccgcagc ggccgggtca ttgtctacga tcgcgtcatc gccgtctgcc gggtccgcgg
      541 ccgcgccatt ggagacctcc ttcgcgcgct tcgcgcgcca gagggcggaa actgacgctc
      601 aggtgagcgc agcggacaag ctgggaatac gaagcgtaag agcgcccgca tctcctccgg
      661 agtgtcgtaa gccggatcgc ccgcgcaccg catcgcgatg cccggccgcc ttgcgaaccc
      721 aacggccctg ccgatcctga tccttttgat ccgcagcctc gcttgcggcg gcgagcgcct
      781 ggttctgctc ccggatgagc cggcgctcct cgccttcgct atcgaaggcg aatatacgct
      841 gactctccag atcgatcgtg agatcgtccc gagcgaccag ctcgctctcc cacaccgatc
      901 cgaggctgcc cgcctccatc accggccgcc ggaacgcgca gcgcagcatc aaccgccctg
      961 ccgcttccga ggcttctgct ctcccggtcg aggatgagga cgtcggcgtt gttgttgaac
     1021 aacagatcat cgaccgtcag ttgccgatag gccggatcga agtgcggcag cacccagacc
     1081 agcagtccgc cttcggcgcg gtagaaggtc ttgcgtccga taacgacgtc aagaaacgtc
     1141 gtggtgagct gcgcttca
//
LOCUS       DAOMEO010001182         1158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12316_length_1158_cov_2.236627, whole genome shotgun sequence.
ACCESSION   DAOMEO010001182 DAOMEO010000000
VERSION     DAOMEO010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12316_length_1158_cov_2.236627"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLH31_19520"
     CDS             complement(<1..537)
                     /locus_tag="PLH31_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR91417.1"
                     /translation="MSLALATLLYEWRRYMAAVVALAFSGLLVLAQVGMFVGIGKGFT
                     AQIDRARADIMVLGPGAKALFGGPSGLPRRMIPLVYSHPEVTQVAPLDGSGGRFQNIL
                     TPEEQAKIEARTKAAAKGGKQAGGEGGGRKTEFVNVPVIDAIPGYVTVPTDYTQSMVE
                     ALRQPYAVAVDQTALKRLG"
     gene            781..>1158
                     /locus_tag="PLH31_19525"
     CDS             781..>1158
                     /locus_tag="PLH31_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HQR91418.1"
                     /translation="MFRPENVQALAGLALTLGLCWLVSENRKRFPWKLAIGAVIVQVG
                     LVLLLFGLPQAQALLRGVNGAVEGLSASTQAGTMFIFGFLAGGEQPYPVSNAGLGFIF
                     AFRVLPVILVVCALSALLWHWKIL"
BASE COUNT          170 a          387 c          388 g          213 t
ORIGIN      
        1 gcccaggcgc ttgagggcgg tctggtcgac ggccacagcg tagggctggc gcagcgcctc
       61 gaccatcgac tgggtgtagt cggtcggtac ggtgacatag cccggaatgg cgtcgatcac
      121 ggggacgttg acgaactcgg tcttgcgacc gccgccctcg ccgccagcct gcttgccgcc
      181 cttggcggcg gccttggtgc gggcctcgat cttggcctgc tcctcgggtg tcaggatgtt
      241 ctggaagcgc ccgccggacc cgtcgagggg ggcgacctgg gtcacctcgg gatggctgta
      301 gaccagcggg atcatgcggc gcggcaggcc ggacggtccg ccaaacagcg ccttggcccc
      361 ggggccaagc accatgatgt cggcgcgggc gcggtcgatc tgggccgtga agcccttgcc
      421 gatgcccacg aacatgccga cctgggccag gaccagcagg cccgagaacg ccagggcgac
      481 gacggccgcc atgtatcgac gccactcgta caacaacgtg gccaaggcca gcgacatgcg
      541 aacaatgctc ccccaatcaa gggttgcagc aatgaacggc catcagcgaa cggccaagtt
      601 aaatcggggt aaggcggagg actggctctt ttcgggcatc ctgccgccgc cacggcataa
      661 acccctcgtc gggcgtgcga gcacgcaagc cgcttgcctc caacggtaaa cttggtgtca
      721 atctcgcccc ttggctgacg tcgcgggggc gtcgcccagt tcaaaggttc ttgtctcttg
      781 atgtttcgtc ccgagaacgt tcaggccctc gcgggtctgg cgctcaccct cggcctctgc
      841 tggctggtct ccgagaatcg caagcgcttc ccgtggaagc tcgccatcgg cgccgtcatc
      901 gtccaggtcg ggcttgttct gctgctgttt ggcctgccgc aggcccaggc gctgctgcgc
      961 ggcgtcaatg gcgcggtcga ggggctgtcg gcctcgaccc aggccggcac catgttcatc
     1021 ttcggcttcc tggcgggcgg cgagcagccc tatccggtca gcaatgccgg tctgggcttc
     1081 atcttcgcct tccgcgtcct gccggtgatt ctggtggtct gcgccctctc ggcgctgctg
     1141 tggcactgga agatcctc
//
LOCUS       DAOMEO010001183         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12337_length_1157_cov_1.724138, whole genome shotgun sequence.
ACCESSION   DAOMEO010001183 DAOMEO010000000
VERSION     DAOMEO010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12337_length_1157_cov_1.724138"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLH31_19530"
     CDS             <1..368
                     /locus_tag="PLH31_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR91419.1"
                     /translation="IASLAAFAPLPDAPAYSAAKAGLLSYGVALREALRGHGIQVCIA
                     CPGYVTTGMSDQHLGHRPGEISADDAAGRILHALGRDQAVSGFPLGLYWVSRLSLLAP
                     AWVQRLGLQNKDAARCAFP"
     gene            494..>1157
                     /locus_tag="PLH31_19535"
     CDS             494..>1157
                     /locus_tag="PLH31_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91420.1"
                     /translation="MSVTERSLPAIVTNFLAGAKPPKGLGRGAEAYIANLQFRKNITR
                     ISFIALVLVPTVIATLFYGLLAAPRYVSDVKFIVRSVSTQRATGLDMLFRTFGIAKTV
                     DDANVIQKYLQSRDAMQGVIDAGIDVKAIFQRPEADWWSAYPYVWRRDSHESLFDYFA
                     SHVTVGEDSTKGIIELQVETFRPADSLELSKTLLRLAEGMVNKMNERAQRDAMGAANR
                     EVN"
BASE COUNT          197 a          374 c          362 g          224 t
ORIGIN      
        1 tcatcgcctc gctggccgcc ttcgcgccct tgccggacgc acccgcttat tccgccgcca
       61 aggccgggct gctgtcttat ggcgtggcgt tgcgcgaagc cttgcggggc catggcattc
      121 aggtctgcat cgcctgcccc ggctatgtca ccaccggcat gagcgaccag catctgggcc
      181 accggccggg cgagatttcg gccgatgacg ccgccgggcg gatcctgcac gcgctggggc
      241 gggatcaggc cgtttcgggg ttcccactcg gcctctattg ggtctcccgc ctctccctat
      301 tggcgccggc ctgggtgcag cggctgggct tgcaaaacaa ggatgcggct cgatgcgctt
      361 tcccttagga gcggcgtgac cgcaaaattg cttcattatc gtgagatcgc cgctgtggcg
      421 gaacggatgc aggcagctcg gcaatctgct aggttggccc atgttcgaat ggccggcgga
      481 gacaggctaa aggatgagcg taaccgagcg gtccttgccg gcgatcgtga ccaatttcct
      541 cgccggcgcc aagccgccta agggacttgg ccgcggggcg gaagcctata tcgccaatct
      601 ccagttccgg aagaacatca cgcgcatcag ctttatcgcg ctggttctgg tgcctaccgt
      661 gatcgctacc cttttctacg gactgctggc ggcgccgcgc tatgtctccg acgtgaagtt
      721 catcgtgcgc agcgtctcga cccagcgggc caccggcctc gacatgctgt tccgcacctt
      781 cggcatcgcc aagaccgtgg acgatgccaa tgtcatccaa aaatacctcc aatcccgcga
      841 tgccatgcag ggcgtgatcg acgccggcat cgacgtgaag gcgatcttcc agcggcctga
      901 agccgactgg tggtcggcct atccctatgt ctggcgccgg gacagccacg agtccctgtt
      961 cgactatttc gccagccatg tgacggttgg cgaggacagt acgaagggca tcatcgaact
     1021 gcaggtcgag actttccgcc ctgcggattc gctggaactg tccaagacgc tgctgcgcct
     1081 cgccgagggc atggtcaaca agatgaacga gcgcgcccag cgcgatgcga tgggcgcggc
     1141 caatcgcgaa gtgaact
//
LOCUS       DAOMEO010001184         1156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12343_length_1156_cov_2.136240, whole genome shotgun sequence.
ACCESSION   DAOMEO010001184 DAOMEO010000000
VERSION     DAOMEO010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12343_length_1156_cov_2.136240"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>319
                     /locus_tag="PLH31_19540"
     CDS             <1..>319
                     /locus_tag="PLH31_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91421.1"
                     /translation="TALSWPDSSTPWTRLLYALFNGGLVLFGAVIGRRIFTVLGAFGV
                     AVYLGYLAFELFEDSLLFPLSLSALGLALVAAGVWWQRHEDAIQAHIWACLPKAWHAE
                     APRG"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(375..>1156)
                     /locus_tag="PLH31_19545"
     CDS             complement(375..>1156)
                     /locus_tag="PLH31_19545"
                     /inference="COORDINATES: protein motif:HMM:NF036694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HQR91422.1"
                     /translation="RSKSETYNKDEKALEDYLITAGLDDASLHLASGEVRTGADLATV
                     VELARQLRATLNNLHPRYNKAVAEQAVLALAVAAGVLDDPDHATAAVARVATRLDALA
                     DDTERGWVGTADETGLRLSRTVRGVLETNLLDAAFLASPEARRLESIARQLQGVFEGT
                     AVLRRKAEEHPVFGAVDLFDAVTDTGRKGLSLQRYKGLGEMNPEQLWETTLDINARSL
                     LQVKVKEVDAADDLFNRLMGDLVEPRREFIQENALKASVDV"
BASE COUNT          160 a          380 c          398 g          208 t
ORIGIN      
        1 accgcgctgt cctggcccga tagcagcacg ccctggacgc ggctcctgta tgcgctgttc
       61 aacggcgggc tggtgctgtt cggagcggtg atcggacggc gcatcttcac cgtcctgggc
      121 gccttcggcg tcgcggtcta cctgggctat ctggcgtttg agctattcga ggacagcctc
      181 ttgtttcctt tgtccctcag cgcgctgggg ctggcgctgg tggccgccgg cgtctggtgg
      241 caacggcacg aagacgcgat ccaggcccac atctgggcct gcctgcccaa ggcttggcac
      301 gcggaagctc cccgaggccn nnnnnnnnna tcgacgcagt caaagcctcc ccgcgccggt
      361 gagcgaggga gtcctcacac gtcgacgctg gccttcaggg cgttttcctg gatgaactcg
      421 cggcgcggtt ccaccaggtc gcccatgagg cggttgaaca ggtcgtcggc ggcatccact
      481 tccttcacct tcacctggag cagggagcgg gcgttgatgt cgagcgtggt ctcccacaac
      541 tgctcggggt tcatctcgcc caagcctttg tagcgctgga gcgagaggcc tttgcggccg
      601 gtgtcggtga ccgcgtcgaa cagatccacg gcgccgaaca ccggatgttc ctccgccttg
      661 cggcgcagga ccgcggtgcc ctcgaacacg ccctgcaact ggcgggcgat gctctccagg
      721 cggcgggctt ccggcgaggc caggaaggcg gcatccagca ggttggtttc cagcacgcca
      781 cgcacggtac gcgacaggcg caggccggtc tcgtcggcgg tgccgaccca gccgcgctcg
      841 gtgtcgtcgg ccagggcatc caggcgggtc gccacgcggg cgacggcggc ggtggcatga
      901 tcgggatcgt ccagcacgcc ggcggcgact gccagggcca gcacggcctg ttcggcgacg
      961 gccttgttat agcgcggatg cagattgttt agcgtggcgc gcaactggcg ggccagttcc
     1021 accactgtcg ccagatcggc gcccgtgcgc acctcgccgg acgccagatg cagggaggca
     1081 tcgtcaaggc cggcggtgat aaggtagtcc tctagcgcct tctcgtcctt gttgtaggtc
     1141 tcggacttgc tgcgcg
//
LOCUS       DAOMEO010001185         1154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12371_length_1154_cov_2.327571, whole genome shotgun sequence.
ACCESSION   DAOMEO010001185 DAOMEO010000000
VERSION     DAOMEO010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12371_length_1154_cov_2.327571"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLH31_19550"
     CDS             complement(<1..609)
                     /locus_tag="PLH31_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HQR91423.1"
                     /translation="MLYALHEAAYYASSPLREAARVARDFWGSPLNPAANTDFGRRIH
                     AGADLFTNVTRRYGKPAWNIDTIKVGQVDVRVRPTVVWESPWARVIQFDRDMADMRRA
                     GKFSLDPAVLIVAPLSGHYATLLRGTVEAFLPDHAVFIVDWVNAREVSMLEGRFDFHD
                     YIDHIIQMLAVLGPRPNVVAVCQPGPPVLAAAALMAEDNHPSR"
     gene            complement(710..>1154)
                     /locus_tag="PLH31_19555"
     CDS             complement(710..>1154)
                     /locus_tag="PLH31_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQR91424.1"
                     /translation="TTEKALNGKWSAVFFGFTYCPDVCPGTLQGLAAATDILGPKKAE
                     DLQIVFISVDPERDDAKQMKAYVSADYVPKTTLGLTGTPQQVAAAAKAYRVYFAKVGD
                     GPGYTIDHSTAIYLMDPKGRFSTVIPYNLPPEDIARRINDAMREG"
BASE COUNT          204 a          348 c          405 g          197 t
ORIGIN      
        1 ccgcgagggg tggttgtcct cggccatcag ggcggcggcg gccaggaccg gcggtccggg
       61 ctggcagacg gccacgacat tggggcgcgg gcccagcacg gccagcatct ggatgatgtg
      121 gtcgatatag tcgtggaagt cgaaccggcc ttccagcatc gagacttcgc gggcgttgac
      181 ccagtcgacg atgaacaccg catggtcggg caggaaggcc tcgaccgtac cgcgcaacaa
      241 ggtcgcgtag tgacccgaca gcggcgcgac gatcagcacg gcggggtcga ggctgaactt
      301 gccggcccgg cgcatgtcgg ccatgtcgcg gtcgaactgg atgacccggg cccaggggct
      361 ttcccagacc acggtggggc gcacccgcac atcgacctgg ccgaccttga tggtgtcgat
      421 gttccaggcc ggcttgccgt agcggcgcgt gacattggtg aacaggtcgg cgccggcatg
      481 gatgcggcgg ccgaaatcgg tgttggcggc ggggtttagc ggcgagcccc agaagtcgcg
      541 ggcgacacgg gccgcttcac gcagggggga ggaggcgtaa taggccgctt catgaagggc
      601 gtagagcatg gcgcatccaa tatgttgcgt cgcagcataa catatcgaag cctaagaaag
      661 tccatccaag aaacgtgcgg cccgtcgcct cagtccgcct aaactgagat cagccctcgc
      721 gcatggcgtc gttgatgcgc cgggcgatgt cttccggcgg caggttgtag gggatcaccg
      781 tcgagaaccg gcccttggga tccatcagat agatcgcggt cgagtggtcg atcgtatagc
      841 cgggtccatc ccccaccttg gcgaaataga cgcgataggc tttcgccgcc gccgccacct
      901 gctgcggggt tcctgtcaga cccagggtcg ttttgggcac atagtcggcc gagacatagg
      961 ccttcatctg cttggcgtcg tcgcgctcgg gatcgaccga gatgaagacg atctgcagat
     1021 cttcggcctt cttggggccc aggatatcgg tggccgccgc caggccctgc agggtgccgg
     1081 ggcagacgtc cgggcaatag gtgaagccga agaacaccgc gctccacttg ccgttcaggg
     1141 ccttctccgt ggtc
//
LOCUS       DAOMEO010001186         1151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12417_length_1151_cov_2.594891, whole genome shotgun sequence.
ACCESSION   DAOMEO010001186 DAOMEO010000000
VERSION     DAOMEO010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12417_length_1151_cov_2.594891"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..674
                     /locus_tag="PLH31_19560"
     CDS             342..674
                     /locus_tag="PLH31_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HQR91425.1"
                     /translation="MATIGTFIKRGDDLHGAIRTLTLDVDLVMRPVPKTRDGPDFLIY
                     AAGTAKGPNGIDTQLGAGWKRTCDQNCLHIDVELDDPAFPGPLSATLVEDHVAPSGER
                     FSMVWARA"
     gene            909..>1151
                     /locus_tag="PLH31_19565"
     CDS             909..>1151
                     /locus_tag="PLH31_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91426.1"
                     /translation="MTKDLESPPGQLDPSDKSGTSEEAWIVGSNPEQAIRMRSLSVYL
                     SNGGALKDVRLVEGEDERWTMWVRLINRPGEYRLNLF"
BASE COUNT          228 a          376 c          349 g          198 t
ORIGIN      
        1 ggtcaggacg tgccccaaat aaggcctttt tctgtataaa agtcactttg gaaccgccca
       61 aacggcccgg cgtttctccc gaggagatcg ccaccacctc ctccggttga gccccgggtc
      121 cttggcgatc ttcatcgctg gacgcctact cggcgccggc tgacgccgcg ccccgatccc
      181 acagcgcgcc gtcttagact tgcttccccg gcctcttggc ctctgtgcgg gacaaggaaa
      241 cacactttgg cagcgcaccc gctcggggtg cgttgtcgac tccgggggat gggccatcgg
      301 gaccgcgacc agcgcgtctc gtcacatgca ggagatctct gatggcgacc atcggtactt
      361 tcatcaaacg tggcgacgat ctgcacggcg cgatccgcac cctgaccctg gatgtcgacc
      421 tggtgatgcg ccctgtcccg aagaccaggg acggccccga cttcctgatc tacgccgccg
      481 gcaccgccaa aggcccgaac ggcatcgaca cccagctcgg agccggctgg aagcggacct
      541 gcgatcagaa ctgcctgcac atcgacgtcg aactcgacga tccggccttc ccgggaccgc
      601 tctccgccac cctggtcgaa gaccacgtcg cgccgagcgg tgaacgcttc agcatggtct
      661 gggcgcgcgc ctaaacgggc aaaacaagga cgggggcgtt gcggggaccg ccccgctgga
      721 gggggtgacc agagacccta gaggctcggg tccaggggga gggcgctccc cccaaaactc
      781 aacctaaagg tcgggtttga tgaagaataa gaccgttcaa tctgcaatag ggcggacttg
      841 ggtttgctga tattttgaag tggcctagcc ggacaatcga gcgccagtta atcgggggga
      901 tgctttcatt gaccaaagac ctcgaatccc ctccgggcca acttgatccc agcgacaaga
      961 gcgggaccag cgaagaagcc tggatcgtag ggtccaaccc cgaacaggcc atccgtatgc
     1021 ggagcctctc agtctatctc agcaatggcg gcgccctgaa ggatgtccgc ctggtcgaag
     1081 gcgaggacga acgctggacc atgtgggtgc ggctgatcaa ccggccgggc gaatatcgcc
     1141 tcaacctctt c
//
LOCUS       DAOMEO010001187         1150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12431_length_1150_cov_2.394521, whole genome shotgun sequence.
ACCESSION   DAOMEO010001187 DAOMEO010000000
VERSION     DAOMEO010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12431_length_1150_cov_2.394521"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="sufC"
                     /locus_tag="PLH31_19570"
     CDS             complement(<1..483)
                     /gene="sufC"
                     /locus_tag="PLH31_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQR91427.1"
                     /translation="MLNIQNLHARVEDKAILKGVTLDVPAGEVHAIMGPNGAGKSTLS
                     YVLTGRGGYEVTEGSTSLNGEDLLALDANERAAKGLFLSFQYPLEIPGVPALTFIRTA
                     LNAQRRARGEDEIAAPAFLKLAREKAASLKIDFEMLKRGLNVGFSGGEKKRMEIFQMA
                     M"
     gene            complement(476..778)
                     /locus_tag="PLH31_19575"
     CDS             complement(476..778)
                     /locus_tag="PLH31_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91428.1"
                     /translation="MIPVRFGLNDKEYKYARQLAYQAAHGTWINPYGDEAPLIDRSAK
                     LLANGNADAAAERALLIELLKLAAYSPEHEWEAPALTGKPTTFAIQTLEKIMAFNA"
     gene            complement(775..>1150)
                     /locus_tag="PLH31_19580"
     CDS             complement(775..>1150)
                     /locus_tag="PLH31_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQR91429.1"
                     /translation="IVAKGISAGKSTSTYRGLVSAHPKAKGARNFTQCDSLLIGKTCA
                     AHTIPYIEARNGQSVFEHEATTTRLSDDQLFYCQQRGLNQEEAVALLVNGFVKDVLQQ
                     LPMEFAVEAQKLVAISLEGSVG"
BASE COUNT          181 a          361 c          382 g          226 t
ORIGIN      
        1 catcgccatt tgaaagattt ccatccgctt cttctcgccg cccgagaagc ccacgttcag
       61 gccgcgcttg agcatctcga agtcgatctt cagcgaggcg gccttctcgc gggccagctt
      121 caggaaggcc ggcgcggcga tctcgtcctc gccccgcgcg cggcgctggg cgttgagggc
      181 ggtgcggatg aaggtcaggg ccggaacgcc cggaatttca agtggatact ggaaagacag
      241 gaacaggccc ttggcggccc gctcattggc gtccagggcc agaagatcct cgccattcag
      301 gctggtcgag ccctcggtga cctcatagcc gccacggccg gtcaggacgt aagacagggt
      361 cgatttgccc gccccgttcg gccccatgat ggcgtgcacc tcgccggccg gaacgtccag
      421 cgtgacgccc ttcaggatag ccttgtcttc gacgcgggcg tggaggttct ggatattaag
      481 cattgaaagc catgatcttt tcgagcgtct ggatagcgaa cgtggtcggc ttgccggtca
      541 gggccggcgc ctcccattca tgttcggggc tgtaggcggc gagtttgagc agttcgatca
      601 gcagggcgcg ctccgcggcc gcgtcggcgt tgccgttcgc cagcagcttg gcgctgcggt
      661 caatcagcgg ggcctcatcg ccatacggat tgatccacgt gccgtgcgcc gcctgatagg
      721 ccagctgacg cgcatacttg tattccttgt cgttcagccc gaagcggaca gggatcatcc
      781 cacgctccct tccaggctga tcgccaccag cttctgggcc tcgacggcga actccatcgg
      841 cagctgctgc agcacgtcct tgacgaagcc gttgaccagc agggctacgg cctcttcctg
      901 gttcaggccg cgctgctggc aatagaacag ctgatcgtcc gacaggcggg tggtggtggc
      961 ctcgtgctcg aacaccgact ggccgttgcg ggcctcgata taggggatgg tgtgggccgc
     1021 gcaggtcttg ccgatcagca ggctgtcgca ctgggtgaag ttgcgggcgc ccttggcctt
     1081 ggggtgagcc gacaccaggc cgcgataggt gctggtcgac ttgccggcgc tgatgccctt
     1141 ggcgacgatc
//
LOCUS       DAOMEO010001188         1149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12448_length_1149_cov_2.247715, whole genome shotgun sequence.
ACCESSION   DAOMEO010001188 DAOMEO010000000
VERSION     DAOMEO010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12448_length_1149_cov_2.247715"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..767
                     /locus_tag="PLH31_19585"
     CDS             9..767
                     /locus_tag="PLH31_19585"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HQR91430.1"
                     /translation="MDGLKPGNRQAGRQQGQQQQSELGAMIQEQQRLRDRTYREGRPG
                     QQGQQGQRGQRGQQGQQGQDGQEGEGAFGDLQQGQQELRRRLGQMLEQLKRQQQGQQG
                     QGQQGQGQQGQGGEGADPSGRAGEAFGRAEQAMRDAEGALGEGNGQGALDAQGRALQA
                     LRQGAQAMAEGQQGDQNGPGPGGTPGESAERTDPLGRPMRTQDYGDDFSVKVPDEVDA
                     QRARQVLEELRRRLEQTERPQQELDYIERLLRNF"
     gene            complement(846..1076)
                     /locus_tag="PLH31_19590"
     CDS             complement(846..1076)
                     /locus_tag="PLH31_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013267843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91431.1"
                     /translation="MPIQVRLAVLLAERGVKSKDLAEHVGITEANLSLLKQGKVKGVR
                     FETLERICQYLDCQPGDLLRYEPGPVAVEPGT"
BASE COUNT          211 a          377 c          417 g          144 t
ORIGIN      
        1 aggcgatgat ggacggcctc aagcccggca atcgccaggc cggccgccag cagggccaac
       61 agcagcagag cgaactgggc gccatgatcc aggagcagca gcgcctgcgc gaccgcacct
      121 atcgcgaagg ccgccccggc cagcaaggac agcagggcca acggggtcag cgcggccaac
      181 agggccagca gggacaggac gggcaggagg gcgagggcgc cttcggcgac ctccagcagg
      241 gccagcagga gttgcgtcgg cgtctcggcc agatgctgga gcagctcaag cgccagcagc
      301 agggtcaaca gggtcaggga caacagggac aaggccagca ggggcagggc ggcgagggcg
      361 ccgatccgtc cggccgcgcc ggagaggcct tcggtcgggc cgagcaggcc atgcgcgatg
      421 ccgaaggggc actgggcgag ggcaatggcc agggcgcgct cgatgcccag ggccgcgccc
      481 ttcaggcgct gcggcagggt gcccaggcca tggccgaggg ccagcagggc gaccagaacg
      541 gccccggccc tggcggtacg ccgggtgaga gcgccgagcg caccgatccc ctcggccgcc
      601 ctatgcgaac ccaggattat ggcgacgact tctcggtgaa ggtgcccgac gaggtggacg
      661 cccagcgcgc ccgccaggtg ctggaagaac tgcgccgccg cctggaacag accgagcggc
      721 cgcagcagga actggattat atcgagcgcc tgctgcgcaa tttctgaggg ctggacgcga
      781 gatctcgggc gggatcggga tcgcctcccg gtctatccag acgcgcgcgg cagcgacccc
      841 ttcgcttagg tgccgggttc gaccgccacc ggccccggtt cgtagcgcag caggtctccg
      901 ggttggcagt cgagatactg gcagatgcgc tcgagcgtct cgaagcgaac gcctttcacc
      961 ttgccctgct tgagcaggga caggttggcc tcggtaatcc ccacatgttc ggcgagatcc
     1021 ttggatttga cgccgcgctc cgcaagaagc accgccagcc ggacttggat cggcatgggt
     1081 gtccctcaca cgaattgctg gttctcgtcg gacaaagcga tggcttcgcc catgatccag
     1141 gccatgacg
//
LOCUS       DAOMEO010001189         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12462_length_1148_cov_2.757548, whole genome shotgun sequence.
ACCESSION   DAOMEO010001189 DAOMEO010000000
VERSION     DAOMEO010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12462_length_1148_cov_2.757548"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLH31_19595"
     CDS             <1..287
                     /locus_tag="PLH31_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91432.1"
                     /translation="ELPTLINGHKLKVQFDLRNVLNLIDRDWGKVEEYGDSVTLARAA
                     CVSSTGAALTTANNATCPAYVYSSVPSSVTKVRNVEQSLWTMQISLRYEF"
     gene            452..>1148
                     /locus_tag="PLH31_19600"
     CDS             452..>1148
                     /locus_tag="PLH31_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQR91433.1"
                     /translation="MTPTVPAEWAPHRAMWVGFPSHPELWREDLPVAQKEVADLARAL
                     AGPGAERVRLMVVGDEAEAAARALLADTTVEIVRGTFGDIWLRDTGPIYVETAQGPIA
                     AGFEFNGWGGKFEMEGDDIVAEQIAAASGTPLTRNGFVLEGGSLDHDGLGTVLSTRQC
                     LLNENRNGDFDEAKAFAALSRALGVKKVLWLGDGLLNDHTDGHIDNLARFVAPGVVAC
                     PMAFGTDDPNAEVY"
BASE COUNT          194 a          390 c          376 g          188 t
ORIGIN      
        1 aggaactgcc gaccctgatc aacggccaca agctgaaggt ccagttcgac ctgcgcaacg
       61 tgctgaacct gatcgaccgc gactggggca aggttgaaga gtatggcgac agcgtcaccc
      121 tggcgcgcgc ggcctgcgtc agctcgacgg gcgcagccct gaccaccgcc aacaatgcga
      181 cctgcccggc ctatgtctat tcgagcgtcc ccagctcggt gaccaaggtc cgcaacgttg
      241 aacagtcgct gtggacgatg cagatctcgc tgcgttacga gttctaaccc acgcgtcacc
      301 acgacccaca ggaagggcgg ccccgcaacg ggccgccctt tttgttttgt cgcccccatc
      361 tgctagaaaa cgtctttgct agaacaccgc cttgaccccc ggtccgccag gggccatttg
      421 cgccgtccgt tttcatccag gcttgcgaca catgaccccg accgttcccg ccgagtgggc
      481 cccgcaccgc gcgatgtggg tgggctttcc cagccatccc gagctgtggc gcgaagacct
      541 gccggtcgcc cagaaggaag tggcggacct tgcccgcgcc ctggctggcc ccggcgccga
      601 gcgcgtgcgc ctgatggtgg tgggcgatga ggccgaagcc gccgcccggg ctctgctggc
      661 cgacaccacg gtcgagatcg tgcggggcac gttcggcgac atctggctgc gcgacaccgg
      721 cccgatctat gtcgagaccg cgcagggccc gatcgccgcc ggcttcgagt tcaacggctg
      781 gggcggcaag ttcgagatgg agggcgacga catcgtcgcc gaacagatcg cggcggcctc
      841 gggcacgccc ctgacccgca acggctttgt gctggaaggc ggctcgctgg accatgacgg
      901 cctgggcacg gtgctgagca cccgccagtg cctgctcaac gaaaaccgca atggcgactt
      961 tgacgaggcc aaggcctttg ccgcgctgtc cagggcgctg ggcgtcaaga aggtgctgtg
     1021 gctgggcgat ggcctgctga acgaccacac cgacggccat atcgacaacc tggcccgctt
     1081 cgtcgcgccg ggcgtggtgg cttgcccgat ggcgttcggg acggatgatc ccaatgcgga
     1141 agtctatg
//
LOCUS       DAOMEO010001190         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12469_length_1148_cov_1.957914, whole genome shotgun sequence.
ACCESSION   DAOMEO010001190 DAOMEO010000000
VERSION     DAOMEO010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12469_length_1148_cov_1.957914"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLH31_19605"
     CDS             complement(<1..405)
                     /locus_tag="PLH31_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286303.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HQR91434.1"
                     /translation="MLNLREYQRRPASLADFLPWAALVAPGVVLNKDGALQRTAGFRG
                     PDLDSATPAELVSVSARLNGALRRLGSGWAVFVEAQRDPAQAYPDSDFPDPLAGLVDA
                     ERRADFLAAGAHFESDYFLTLTWLPPAERASRI"
     assembly_gap    630..639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<640..895)
                     /locus_tag="PLH31_19610"
     CDS             complement(<640..895)
                     /locus_tag="PLH31_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HQR91435.1"
                     /translation="MEDAASVPGFLAPVRRALTEPMLLAGAPRSVALLNGTLAAAIGL
                     GLRLWLVGLVVWAVGHGLAVWAARRDPLIVEVARRHVRIPA"
     gene            complement(895..>1148)
                     /locus_tag="PLH31_19615"
     CDS             complement(895..>1148)
                     /locus_tag="PLH31_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HQR91436.1"
                     /translation="SAQAAGSSMPWEAPLQKILESIEGPVAKIIAVIIIIVTGLTLAF
                     GDSSGGARKLIQIVFGLSIAFAASSFFLSFFSFGGGALV"
BASE COUNT          235 a          375 c          384 g          144 t
ORIGIN      
        1 gatccggcta gcgcgctcgg ccggcggcag ccaggtcagg gtcaggaagt agtcgctttc
       61 gaaatgcgcc ccggccgcga ggaagtcggc gcgccgttcg gcgtcaacca ggccggccag
      121 cgggtcgggg aaatcgctgt cgggataggc ttgagccgga tcgcgctggg cttcgacgaa
      181 caccgcccaa cccgacccca gccgacgcag cgccccgttc aaacgcgccg acaccgagac
      241 caattcggcc ggggtcgcgg aatccagatc cggcccccga aacccggccg tgcgctgcag
      301 ggcgccgtcc ttgttcaaca ccacgccggg cgcgaccaag gcggcccagg gcaggaagtc
      361 ggccaggctc gcggggcggc gttgatattc gcggaggttc agcatgatcg caggcttcac
      421 tccatcgacg ccagagatgt ccagggctgt catgaagggc atgtacccaa ccttggaccc
      481 aaagtcgtcg tcatcttcag cggcaaagtt cccttcgtac ttgataaagg aggtcatcca
      541 ggtcgcgtaa gagccatggg agtcatcgaa agacagcagt tgggaacaac ttggtacata
      601 catcgttggt gcaggtgaga gggcaggtgn nnnnnnnnng cgcgggaatg cggacgtggc
      661 ggcgcgcgac ctcgacgatc aacgggtcgc gacgcgccgc ccagaccgcc aacccgtggc
      721 cgacagccca gaccaccagc cccacgagcc aaagccgcag gccaagcccg atggcggcgg
      781 caagggtgcc gttgagcagc gccacggacc ggggcgcgcc ggccagcagc atcggctcgg
      841 tcagagcccg gcgaaccggg gccagaaagc cgggcacgga agcggcgtcc tccattagac
      901 cagcgccccg ccgccaaacg agaagaacga caggaagaag gaactggccg cgaaggcgat
      961 cgacaggcca aacacgatct ggatcagctt gcgagcgccg ccggaactgt cgccgaacgc
     1021 cagggtcagg ccggtgacga tgataatgat caccgcgatg atcttggcga ccggcccctc
     1081 gatcgactcc aggatcttct gcagaggcgc ttcccacggc atggacgagc ccgccgcctg
     1141 ggccgagc
//
LOCUS       DAOMEO010001191         1147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12479_length_1147_cov_2.233516, whole genome shotgun sequence.
ACCESSION   DAOMEO010001191 DAOMEO010000000
VERSION     DAOMEO010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12479_length_1147_cov_2.233516"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLH31_19620"
     CDS             <1..1062
                     /locus_tag="PLH31_19620"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQR91437.1"
                     /translation="LMGRYYYRAQNGLAVVSPQGVSNEPKLGQGNAAEQAIIVADAIG
                     HHYRPQGPSDAVPTAPLSVAVALADKLDTLVGFFAIDEKPTGSKDPYALRRSSLGVIR
                     TILENGVRLPLRSAISAAVDAGMAAEQPVIAKLASLDDSQQSEARELLEHHGLDYMRA
                     LSRETGITSGINLHPSVFIYSSFSPGAFAKAFSDHRIFAKSYNLPDKQAELLAFFADR
                     LKVLLKDQGKRHDLVDAVFALGDDDLVRIVARVEALDAFLKTDDGKNLLAGYKRASNI
                     LKAEEKKGWKAEGERGLLDDASAPEAALYDALRMVEPALAAALAKEDFTGAMPLVPGS
                     PRSGRTRLARLSWRSGTDD"
BASE COUNT          221 a          348 c          359 g          219 t
ORIGIN      
        1 ttgatgggtc ggtactacta tcgcgcccag aatggcctcg cggtcgtaag tccccaaggt
       61 gtatcgaatg agcccaagct tggccaagga aacgctgcag agcaggcgat catcgttgcg
      121 gatgcaatcg gtcatcatta tcggccccaa gggccgtctg atgccgtgcc gaccgcccca
      181 ttgagcgtgg cggtggcgct ggccgacaag ctggacacgt tggtcgggtt cttcgcaatc
      241 gacgagaagc cgacgggatc aaaggacccc tatgcgcttc gtcgatcatc gctgggagtc
      301 atccgaacga tcttagagaa tggtgttcgc ctgccactgc gtagcgctat ttccgctgct
      361 gtcgatgcag gaatggccgc tgaacaaccg gtgatcgcca agctagcgtc gctagatgat
      421 agccagcaat cagaggcccg tgagttactt gagcaccacg ggctcgacta catgcgagct
      481 ttatcccgtg agactggaat cacgtcggga attaatctgc acccgtcagt cttcatatat
      541 tcgtccttca gccctggagc cttcgctaag gcattttcgg atcaccggat tttcgcgaag
      601 agctataatc tgccagacaa gcaggctgaa ttgctcgcct tcttcgccga ccgcctgaag
      661 gtcctgctca aagaccaagg caagcgccac gacctcgtcg acgccgtgtt cgctctgggc
      721 gacgacgacc tcgtgcgcat cgtcgcccgg gtcgaggcgc tggacgcctt cctgaagacc
      781 gacgacggca agaacctgct ggccggctac aagcgcgcct ccaacatcct caaggccgag
      841 gagaagaagg gctggaaagc cgagggcgag cgcggcctgc tggacgacgc ctcggcgccg
      901 gaagcggccc tctacgacgc cctgcgcatg gtcgagccgg ccctggcggc ggctttggcc
      961 aaggaagact tcaccggcgc gatgccgttg gtgccagggt ctcctcgatc tggccgtact
     1021 cgcttggcgc gcctgtcgtg gcggtctgga acagatgatt gaggcccggc agggcgacga
     1081 cggtgacatc ccggctctgg gtcagggcag ccttgatggc cggcaggatc cgtttggccg
     1141 agacctg
//
LOCUS       DAOMEO010001192         1146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12495_length_1146_cov_2.821265, whole genome shotgun sequence.
ACCESSION   DAOMEO010001192 DAOMEO010000000
VERSION     DAOMEO010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12495_length_1146_cov_2.821265"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="hisC"
                     /locus_tag="PLH31_19625"
     CDS             <1..1079
                     /gene="hisC"
                     /locus_tag="PLH31_19625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQR91438.1"
                     /translation="PVRAALETVQAYKSGMTLAEAGRRAGRHDLSKLASNENLLGPSP
                     TIAAAVQSALASPEIYPDPYCETLRDALGSHLSVDPARIVVSPGSEALIDYLFRAVIA
                     PGETILMSSPTFPAYEIFAKCAEATIVDVPRLANFDLDVEAFKVEAAKGPKLLVLCTP
                     NNPTGNALTGVDIGEILAVTPRSTLVFVDEAYREYHEAFNTFALLEAWGGPWVSARTF
                     SKAYGLAGMRVGYGIASSAQLVGYLDRLRPPFNVTSISQAAALAALADREHLERTVAL
                     TIAERGRVEAALDAMGIEHTESEANFVFLRSPAGPEATTARLLQEGLIIRPTPVAGGW
                     VRITIGRPADNDRLIAALPAALAR"
BASE COUNT          170 a          424 c          359 g          193 t
ORIGIN      
        1 cgcccgtccg cgcggcgctg gaaaccgtcc aggcctacaa gtccggcatg accctggccg
       61 aggcgggtcg tcgcgcgggc cgccacgacc tgtccaaact ggccagcaac gagaacctgc
      121 tgggccccag cccgaccatc gccgccgccg tgcagtctgc gctggcctcg cccgagatct
      181 atcccgatcc ctattgcgag accctgcgcg acgccctggg ctcgcacctc tcggtcgatc
      241 cggcccgcat cgtggtgtcg ccgggctcag aagccctgat cgactatctg ttccgggccg
      301 tgatcgcgcc gggcgagacg atcctgatgt cgagcccgac cttcccggcc tacgagatct
      361 tcgccaagtg cgccgaggcg accatcgtcg acgtgccgcg cctggccaat ttcgacctcg
      421 acgtcgaagc cttcaaggtc gaggccgcca agggccccaa gctgctggtc ctgtgcacgc
      481 ccaacaatcc gaccggcaac gccctgaccg gcgtcgatat cggcgaaatc ctggcggtga
      541 cgccccggtc gaccctggtc ttcgtggacg aggcctatcg cgagtatcac gaggccttca
      601 acaccttcgc cctgctggag gcctggggcg ggccgtgggt ttcggcccgc accttctcca
      661 aggcctatgg tctggccggc atgcgggtgg gttacggcat cgcctcgtcg gcgcagctgg
      721 tcggctatct ggatcgcctg cgtccgccct tcaacgtcac ctcgatcagc caggccgcag
      781 ccttggccgc cctcgctgat cgcgaacacc ttgagcgcac cgtcgccctt acgatcgccg
      841 aacgtggccg ggtcgaggcg gcgctggacg ccatgggcat cgagcacacc gagagcgagg
      901 ccaactttgt gttcctgcgc tcgccggctg gcccggaggc cacgaccgcg cgtttgctgc
      961 aggaagggct gatcattcgc ccgacgcccg tcgccggcgg ctgggtccgt atcaccatcg
     1021 gccgaccggc cgacaatgac cgcctgatcg ccgccctgcc ggccgcgctg gcgcgctgag
     1081 ttctgggagc taaaccatga ccatgaccgc ccaaaacccg ctcggccttg atggtttcga
     1141 gttcgt
//
LOCUS       DAOMEO010001193         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12511_length_1145_cov_2.631193, whole genome shotgun sequence.
ACCESSION   DAOMEO010001193 DAOMEO010000000
VERSION     DAOMEO010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12511_length_1145_cov_2.631193"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="PLH31_19630"
     CDS             complement(<1..>1145)
                     /locus_tag="PLH31_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR91439.1"
                     /translation="GFDYTVKAATQVERQLEKYRADGVAERTVISVPRFGQSQFNTAN
                     GVLVLNSAGDRKQSADQIAAELNKTLSKITGVRAVASVRGAFQRGGGGGNSVDLIAVG
                     NDYVELANWLKPILAEAQANPGLSRPRIDYEPTSPRLSVQIDRDKAGTLGVSAQSIGR
                     ALETMFGSRRATTYIKNGQEYDVILQSTLEQRRSIADLDQLQVRTQSGALVPLSTVVT
                     TELRGDTPDRPRVDRLRSVTLTTQLAEGYTVADAVAFLQEQAEARPTPGVSIKWGGQA
                     KDYLEASGAVGVAFGLALLLVFLVLAAQFESWIHPAVIMLTVPLAVLGGLFGLLITGS
                     SINTYSQIGLIILVGIAAKNGILIVEFANQLRDEGLKVKEAVIEAAAL"
BASE COUNT          196 a          366 c          381 g          202 t
ORIGIN      
        1 agggcggccg cctcgatcac cgcctccttc accttcaggc cttcgtcgcg cagctggttg
       61 gcgaactcga cgatcaggat gccgttcttg gcggcgatgc cgaccaggat gatcaggccg
      121 atctgactgt aggtgttgat gctcgatccg gtgatcagca ggccaaacag gccgcccagc
      181 accgccaagg gtacggtcag catgatgacg gccgggtgga tccagctctc gaactgcgcc
      241 gccagcacca ggaagaccag cagaagcgcc aggccgaagg cgacgccgac ggcgccggaa
      301 gcttccagat agtccttggc ctgaccgccc cacttgatgc tgacgccggg ggtgggccgc
      361 gcttcggcct gttcctggag gaaggccacc gcgtcggcga cggtatagcc ttcggccagc
      421 tgggtcgtca gggtcaccga acgaagacga tcgacacgcg ggcgatcagg cgtatcaccg
      481 cgaagctccg tggtgaccac ggtcgacagc ggcaccaggg cgcccgattg cgtgcgcacc
      541 tgtagctggt ccagatcggc gatgctgcgc cgctgttcca gggtggactg caggatgacg
      601 tcgtattcct ggccgttctt gatgtaggtg gtagcccgac gggagccgaa catggtctct
      661 agcgcccggc cgatcgactg agcggacacc cccagcgtac cggccttgtc tcgatcaatc
      721 tgcaccgaaa gacgcggcga ggtgggctca tagtcgatgc ggggacgaga caagcccggg
      781 ttggcctggg cttcggccag gatgggcttg agccagtttg ccagctcgac ataatcattg
      841 ccgaccgcga tcagatcgac gctgttgccg ccgccgccgc cgcgttggaa ggcgccccgc
      901 acgctggcca cggcgcgcac gccggtaatc ttgctgagcg tcttgttcag ttcggctgcg
      961 atctggtcgg cgctctgttt cctgtcgccg gcgctattga gcaccagcac gccgttagcg
     1021 gtattgaact gactctggcc aaagcgcggc accgagatga cggtgcgctc cgccacgccg
     1081 tcggcgcgat acttctcaag ctggcgctcg acctgggtgg cggccttgac ggtatagtca
     1141 aagcc
//
LOCUS       DAOMEO010001194         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12527_length_1145_cov_1.611009, whole genome shotgun sequence.
ACCESSION   DAOMEO010001194 DAOMEO010000000
VERSION     DAOMEO010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12527_length_1145_cov_1.611009"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="PLH31_19635"
     CDS             complement(<1..123)
                     /locus_tag="PLH31_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012289022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine monophosphate synthase"
                     /protein_id="HQR91440.1"
                     /translation="MTFAPRMARGIALPNLLFFTDPARVPDPDVVAERLPRGAGV"
     gene            179..843
                     /locus_tag="PLH31_19640"
                     /pseudo
     CDS             179..843
                     /locus_tag="PLH31_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
     gene            complement(840..>918)
                     /locus_tag="PLH31_19645"
     CDS             complement(840..>918)
                     /locus_tag="PLH31_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91441.1"
                     /translation="ALAREDLEALLAMAQPGDAIEIAAD"
     assembly_gap    919..928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<929..>1145)
                     /locus_tag="PLH31_19650"
     CDS             complement(<929..>1145)
                     /locus_tag="PLH31_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91442.1"
                     /translation="GPTTALPIKPMAPDDGWCDAPGDPAYNRPVKLPYPASAERLWRD
                     DRVYDLVGILAHNDDPPVPGMGSAIFLH"
BASE COUNT          182 a          379 c          388 g          186 t
ORIGIN      
        1 cacgcccgcc ccacgcggca ggcgctcggc gacaacgtcc ggatccggaa ctcgcgctgg
       61 atcggtgaag aaaagcaggt ttggcagggc gatcccgcgc gccatccttg gcgcgaaggt
      121 catggccgtc ctagacaggg cttccagttc atacatcgat gtcaagcggt cgccctccat
      181 gtccgacgcc ctccaatcaa gccccctggc cgccatccgc gcccggatcg ccgccgctga
      241 aaaggctgcg ggccgtgtgc cgggttcggt cacgctggtg gcggtctcca agacccagcc
      301 ctgggaccat atcgccccgg ttctggccgc tgaccagcgg gtgttcggcg aaaaccgcgt
      361 tcaggaggcc atggagcgct ggacccggcg gatgttcgct ggcacctcgt cggtcatttg
      421 cagaccaaca aggtccgcga ggcggtcggc ttcttcgacg tgatcgagac cgtggaccgc
      481 gaaaaactgg cccgggccct cgccgacgag atcgacaagc agggcaaggc cccgcgcctc
      541 tatgtgcagg tcaatatcgg ccaggaaccg cagaaggcgg gcgttgctcc ggccgaggcc
      601 gacgccttca tcgccctttg ccgcgcgctt ggcctgacga tcgaggggtt gatgtgcatc
      661 ccgcccgccg aggatgatcc ggctccgcat ttcgccgccc tgcggaagat cgccgcccgt
      721 aacgggatcg aaaggctgtc gatgggcatg agcgacgatt tcgaggtcgc gattgccgaa
      781 ggggctacgt cggtgcgggt cggctcggcg atctttggat cgcgacgcta cccgccagcc
      841 tagtccgccg cgatttcgat cgcatccccc ggctgcgcca tcgccagcag ggcctcaaga
      901 tcttcgcggg ccagggccnn nnnnnnnnga tgcaggaaga tcgccgagcc catgcctgga
      961 accggcggat catcattatg cgccaggatt ccgacgaggt cgtagacgcg gtcgtcgcgc
     1021 catagccgct cggcgctggc cgggtagggc agcttgaccg gccggttata ggccgggtcg
     1081 cccggcgcgt cgcaccagcc gtcgtcgggg gccattggct tgatggggag ggcggtggtc
     1141 gggcc
//
LOCUS       DAOMEO010001195         1144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12534_length_1144_cov_2.674013, whole genome shotgun sequence.
ACCESSION   DAOMEO010001195 DAOMEO010000000
VERSION     DAOMEO010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12534_length_1144_cov_2.674013"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLH31_19655"
     CDS             <1..1045
                     /locus_tag="PLH31_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQR91443.1"
                     /translation="AILIGGVLWFIDKQRFEATDNAFVQADTVQISPQVSGAIIEILV
                     ADNQRVEVGQVVARIDPATFQARLDQAIANAQALDAAVCAVDDKALLEQALIAQRAAG
                     VSSAQADAGRARADLERYDSLANQGWVSQQKVQTSRASATQSAAAVASAQAALEAERR
                     AAQSLGSARAQTAAQASAAQAAVAQARLDLDRTVLRAPVAGVIGARAVRPGQLVQPGA
                     VLMSIVPLGQSYVVANFKETQVSRLRVGQSVQIKADAFGKQAIHGKVDSFAPATGQEF
                     ALIPVENAVGNFTKITQRLPVKIAVDPSRLGGALRPGLSVEVKVDVRDRSGPSFAEAG
                     LAPAQVARQGAAR"
BASE COUNT          165 a          418 c          405 g          156 t
ORIGIN      
        1 ggcgatcctg atcggcggcg tgctttggtt catcgacaag cagcgctttg aggcgaccga
       61 caacgccttc gtccaggccg acaccgtgca gatcagccca caggtgtctg gcgcgatcat
      121 cgagatcttg gtcgcggaca accagcgggt cgaggtcggc caggtggtgg cgcggattga
      181 ccccgcgacg ttccaggccc ggctggacca ggccatcgcc aacgcccagg ccctggacgc
      241 cgccgtgtgc gcggtcgacg acaaggcctt gctggagcaa gccctgatcg cccagcgggc
      301 ggcgggcgtc tcgtccgccc aggccgacgc cggacgcgcc agagccgacc tggagcgcta
      361 tgacagcctg gccaatcagg gctgggtctc gcagcagaag gtgcagacgt cgcgcgccag
      421 cgccacccag tcggccgccg ccgtggccag cgcccaggcg gcgctcgagg ccgaacgtcg
      481 cgcggcccag tctctgggtt cggcccgggc ccagacagcg gcccaggcct cggcggccca
      541 ggccgctgtc gcccaggcgc ggctggatct ggaccgcacc gtgctgcggg cgccggtcgc
      601 aggcgtgatc ggcgcccgcg cggtgcggcc gggccagctg gtccagcccg gcgcggttct
      661 gatgtcgatc gtgccgctgg gccagagcta tgtggtcgcc aatttcaagg aaacccaggt
      721 ctcgcgcctg cgcgtgggtc agtcggtcca gatcaaggcc gacgcctttg gcaagcaagc
      781 catccacggc aaggtcgaca gcttcgcgcc cgccaccggt caggagttcg ccctgatccc
      841 agtcgagaac gcggtgggca atttcaccaa gatcacccag cgcctgccgg tcaagatcgc
      901 ggttgatccc tcccggctgg gcggcgccct gcggccgggc ctgtcggtcg aggtcaaggt
      961 cgatgtgcga gaccgctcag gcccctcctt cgccgaggcg ggcctggcgc cggcccaggt
     1021 ggcccgccag ggcgccgcgc gctaggcgcc ggcgatgacc gacgccgccc cgcccgctcc
     1081 cgggggccta gccccggccg ttccggcaaa gaccctgagc caggccgact ggaccaagct
     1141 gttc
//
LOCUS       DAOMEO010001196         1144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12537_length_1144_cov_2.202938, whole genome shotgun sequence.
ACCESSION   DAOMEO010001196 DAOMEO010000000
VERSION     DAOMEO010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12537_length_1144_cov_2.202938"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1144
                     /locus_tag="PLH31_19660"
     CDS             <1..>1144
                     /locus_tag="PLH31_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR91444.1"
                     /translation="NRIGNGVVGDVQVQLFGKLVRSDLARLRGAHSGSYVSSVLYDAS
                     LIREAATSGVVNYTREFLTVIGALAVMFSLDPLLAAGVLVIAPVASLIMRRFARKTTK
                     AAKGAMGETSNLSTAIMESLDGVKIVKMENREAYEEARVAAVVDRRQHHLIKGSNARA
                     MAAPATETFTTLITAGVFVYAGWRATTGDITAGAFFAFLSALLMAAQSLRQVANLQTV
                     FSEGLTAARRLFEALDVAPTITDPADARALPPGDSAIALERVSFSYGGEIAALTEVSL
                     EVRRGETVALVGPSGGGKSSILNLIPRFYDVTAGAVTIDGHDVRAVTLASLRDRIALV
                     TQEPFLFDDTIRANIAYARPDATEAQIIAAARQAAAHDFITALPDGY"
BASE COUNT          169 a          416 c          376 g          183 t
ORIGIN      
        1 aaccgcatcg gcaatggcgt ggtcggcgac gtgcaggtgc agctgtttgg caagctggtg
       61 cgctcggacc tggcccggct gcgcggcgcc cattcaggat cctatgtctc ttcggtgctg
      121 tacgacgcca gcctgatccg cgaggcggcg accagcgggg tggtcaacta cacgcgcgag
      181 ttcctgaccg tgatcggcgc cctggcggtg atgttcagcc tcgacccgct cctggccgcc
      241 ggcgtgctgg tgatcgcccc ggtcgccagc ttgatcatgc ggcgcttcgc caggaagacg
      301 accaaggccg ccaagggcgc catgggcgag acctcgaacc tgtcgaccgc gatcatggag
      361 agcctggacg gggtcaagat cgtcaagatg gagaaccgcg aggcctatga ggaggcccgc
      421 gtcgccgccg tggtcgatcg ccgccagcat cacctgatca agggctccaa cgcccgggcc
      481 atggccgccc cggccaccga gaccttcacg accctgatca ccgccggggt cttcgtctat
      541 gccggctggc gggccacaac gggcgacatt accgccggcg ccttcttcgc ctttctcagc
      601 gcgctgctga tggccgccca gtcgctgcgt caggtggcca acctgcagac ggtgttcagc
      661 gaaggcctga ccgccgcccg ccggctgttt gaggctctgg acgtggcccc gaccatcacc
      721 gatcccgccg acgcccgggc tctgcccccg ggcgacagcg ccatcgcgct cgagagggtc
      781 agcttctcct atggcggcga gatcgcggcc ctgacggagg tttcgcttga ggtccggcgc
      841 ggcgagaccg tggccctggt cggcccgtcc ggcggcggca agagctcgat cctcaacctg
      901 atcccgcgct tctatgacgt caccgccggc gcggtgacca tcgacggcca tgacgtgcgc
      961 gcggtgaccc tggcctccct gcgcgaccgc atcgccctgg tgacccagga gcccttcctg
     1021 ttcgacgaca ctatccgagc caacatcgcc tatgcgcggc cggatgcgac ggaggcgcag
     1081 atcatcgccg ccgcccggca ggccgccgcc catgacttca tcaccgccct gccggacggc
     1141 tatg
//
LOCUS       DAOMEO010001197         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12581_length_1141_cov_2.069982, whole genome shotgun sequence.
ACCESSION   DAOMEO010001197 DAOMEO010000000
VERSION     DAOMEO010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12581_length_1141_cov_2.069982"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>646)
                     /locus_tag="PLH31_19665"
     CDS             complement(<1..>646)
                     /locus_tag="PLH31_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HQR91445.1"
                     /translation="EEQLDLVSDGKLDWKQFLRDFWKDFAAAVGEIAELRVTNVLDAL
                     NDALGPHIFPDKGDGSNPRICPTCGTGQLSLKVGKFGAFIGCSNYPECRFTRQLATSS
                     DGEGEAEAADRELGINPETGRSVWLKNGRFGPYVEEPPAAESGDKPKRSSLPKGWLPA
                     AVDLEKALRLLSLPREVGMHPDDGKKITAGLGRFGPFVLHEGTYANLENPEDVFD"
     assembly_gap    647..656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<657..>1141)
                     /locus_tag="PLH31_19670"
     CDS             complement(<657..>1141)
                     /locus_tag="PLH31_19670"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HQR91446.1"
                     /translation="GRLYELIWKRMIASQMESARIERTTVDIESHDGQTGLRATGQVV
                     LFPGYLTVYEEGRDDDGDEDSARLPMITEGAAAKVIDARADQHFTEPPPRYSEASLVK
                     KMEELGIGRPSTYASVLTVLRDREYVKMEKNRFIPEDKGRLVTAFLEQFFRRYVEYDF
                     T"
BASE COUNT          168 a          362 c          385 g          216 t
ORIGIN      
        1 gtcgaacacg tcctcggggt tttccagatt ggcgtaggtg ccctcgtgca gcacgaaggg
       61 cccgaagcgg ccaaggccgg cggtgatctt cttgccatcg tcggggtgca tcccgacctc
      121 gcggggcagg gacagtaggc gcagggcctt ttccagatcg acggcggccg gcagccagcc
      181 cttgggcagg cttgatcgct tgggcttgtc gccgctctcc gccgccggcg gctcttcgac
      241 ataggggcca aagcggccgt tcttcagcca caccgagcga ccggtttccg ggttgatccc
      301 cagctccctg tcggcggctt cagcctcgcc ttcgccgtcc gacgaggtgg cgagctggcg
      361 ggtgaagcgg cattcgggat agttggagca gccgataaag gccccgaact tgccgacctt
      421 cagcgacagc tgcccggtcc cgcaggtggg gcagatgcgc gggttggagc cgtcgccctt
      481 gtcggggaag atgtgcgggc ccagcgcgtc gttcagggcg tccagcacgt tggtaacgcg
      541 cagttcggcg atctcgccga cggcggcggc gaagtccttc cagaagtcgc gcaggaactg
      601 cttccagtcc agcttgccgt ccgacaccag gtccagctgc tcttcgnnnn nnnnnnggtg
      661 aagtcatatt cgacatagcg gcggaagaac tgctccagga aggcggtgac caggcggccc
      721 ttgtcctccg ggatgaagcg gttcttctcc atcttcacat attcgcggtc gcgcagcacg
      781 gtcaggaccg aggcgtaggt cgacggacgg ccgatcccca gctcttccat cttcttgacc
      841 aggctggctt ccgaatagcg cgggggcggt tcggtgaagt gctggtcggc gcgggcgtcg
      901 atcaccttgg cggcggcgcc ttcggtgatc atcggcaggc gggcgctgtc ctcatcgccg
      961 tcgtcgtcgc gaccttcttc gtacaccgtc agatagccgg ggaagagcac cacctggccg
     1021 gtggcgcgca ggccggtctg accgtcgtgg ctttcgatat ctaccgtggt gcgctcgatg
     1081 cgggcgcttt ccatctgcga ggcgatcatc cgcttccaga tcagctcgta caggcggccc
     1141 a
//
LOCUS       DAOMEO010001198         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12627_length_1138_cov_2.249307, whole genome shotgun sequence.
ACCESSION   DAOMEO010001198 DAOMEO010000000
VERSION     DAOMEO010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12627_length_1138_cov_2.249307"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..>314
                     /locus_tag="PLH31_19675"
     CDS             132..>314
                     /locus_tag="PLH31_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQR91447.1"
                     /translation="MSAAPSPVFSVLDLAPVPMGSTVGDALRNSLDLARHAERLGYHR
                     YWLAEHHNMSGIACSAT"
     assembly_gap    315..330
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <331..>1138
                     /locus_tag="PLH31_19680"
     CDS             <331..>1138
                     /locus_tag="PLH31_19680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HQR91448.1"
                     /translation="PGIASAATAVVIGHVAAGTSTIRVGSGGIMLPNHAPLMVAEAFG
                     TLAALYPGRIDLGLGRAPGTDQATMRALRRYMGAVDSFAQDVVELQAWFQPAAPDQSV
                     RAVPGEGQDVPLWLLGSSTYSAQLAAALGLPFAFAAHFAPDQLMDAITLYRRHFKPSA
                     ALAAPYIMICIGVCAAETDDEARRLSSSTQQQFLALRRGRPGLLPPPVDDITQIASQA
                     ELDGLEHTFRYSAVGSPETVRRKIESVLALTGANELMAASQIHDHAARVRS"
BASE COUNT          180 a          379 c          341 g          222 t
ORIGIN      
        1 aagtcccgtc attcgcgcca ccgtccggcg ctttccgcag aattcgcgct ttcagtccga
       61 gatatgcgcc ctgctgtgtc atctgacgcc caaggcttga ggttggcctg aggagacacg
      121 atgttgtctc aatgtcagca gcgccctctc ccgtcttttc cgtcctcgat ctcgcgcccg
      181 tcccgatggg ctccacggtc ggcgacgccc tccgcaacag tcttgacctc gccagacatg
      241 ccgaaaggct tggctatcac cgctactggc tcgccgagca ccacaacatg agcggcatcg
      301 cgtgctcggc cacgnnnnnn nnnnnnnnnn ccgggaatcg ccagcgccgc gaccgccgtg
      361 gtgatcggcc atgtggcggc ggggacgtcg acgattcggg tgggttcggg cggaatcatg
      421 ctgcccaatc acgcgcccct gatggttgct gaggcgtttg gcaccctggc cgccctttat
      481 cccggtcgta tcgatctggg tttgggacgc gcgccgggca cggaccaggc caccatgagg
      541 gccttgcgcc gctatatggg cgcagttgat tcgtttgccc aggatgtggt ggagcttcaa
      601 gcctggttcc agccggcagc gcccgatcag tcagtgcggg ccgtacccgg ggaaggtcag
      661 gacgttccgc tgtggttact aggctccagc acctacagcg cccaactggc agcggcgctg
      721 ggcctgccct tcgctttcgc cgcccatttc gcaccggacc agctgatgga tgcgatcacc
      781 ctgtatcgcc gccatttcaa gccttcagcc gccctggcag caccctatat catgatctgc
      841 atcggcgtgt gcgcggccga gacagacgat gaggcaaggc ggctgtcatc ctccacccaa
      901 caacagtttc tggcgcttcg tagaggtcgg cccggcctgc tgccgcctcc cgtggatgac
      961 attacccaga tcgcctcgca ggctgagctt gacggccttg agcatacctt ccgctattct
     1021 gcggtgggct cgccagagac agtccgcaga aaaatcgagt cggtgctggc cctgaccggc
     1081 gccaatgagc tgatggcggc ctcacagatc cacgatcatg cggcccgggt gcgcagct
//
LOCUS       DAOMEO010001199         1138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12632_length_1138_cov_1.828255, whole genome shotgun sequence.
ACCESSION   DAOMEO010001199 DAOMEO010000000
VERSION     DAOMEO010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_1138_cov_1.828255"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLH31_19685"
     CDS             <1..449
                     /locus_tag="PLH31_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91449.1"
                     /translation="FGDKRNLTLLSDGVAASALGLTLKQQQSLSAIPRTVSVTAANVD
                     ELWSARKRYFFKPAGGHGGKAVYRGDKMTRSVWEQVQQGDYIAQELATPSQRRIRIDG
                     EIKALKLDVRLYTFKGSPLLTASRVYQGQTTNFRTAGGGFAPVFVT"
     gene            complement(501..>690)
                     /locus_tag="PLH31_19690"
     CDS             complement(501..>690)
                     /locus_tag="PLH31_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HQR91450.1"
                     /translation="ELAIAKLPDALKAPLVLTAFDGLSHLEAAAILGVSAKSVETRVY
                     RARKILATTLDPEAFRDH"
     assembly_gap    691..700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<701..1085)
                     /locus_tag="PLH31_19695"
     CDS             complement(<701..1085)
                     /locus_tag="PLH31_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR91451.1"
                     /translation="MTAATPTDEALACQAAKGDERAFALLVGRHKEALYRLLRRYTGD
                     ADEAYEAVHEAFVAAWAALGKYDPSRAFAPWLRTIAINKARDRGRKMAVRRFFFGAAA
                     PDDALAQAIDPGLATDDQLRQQQSMK"
BASE COUNT          208 a          349 c          357 g          214 t
ORIGIN      
        1 tcttcggcga caagcgaaac ctgaccctgt tgtccgacgg cgtcgccgcc agcgctttag
       61 ggctgacgct caaacagcag cagagccttt cggccatccc gcgcaccgtc tcggtgacgg
      121 ccgcgaacgt cgatgagctc tggtcggcgc gaaaacgata ctttttcaaa ccggcgggcg
      181 gccatggcgg aaaggctgtc taccgcggcg acaagatgac ccgcagcgtt tgggaacagg
      241 ttcagcaggg cgactatatc gcccaggaat tggccacgcc gagccaacgc cgtattcgga
      301 tcgacggtga gatcaaggcg ctcaagctcg acgtccgcct ctacaccttc aagggatctc
      361 ccctcctgac cgcctctcgt gtctatcagg ggcagaccac gaatttcaga acggccggcg
      421 gtggatttgc acccgttttc gttacttgat ccttgctggc ggccagcggg agatggccat
      481 cagaccgttt gcgacctgag ctaatgatcg cggaaggctt cgggatccag ggtcgtcgca
      541 aggatcttgc gagcgcgata gacccgggtc tcaaccgact tggctgaaac accaaggatc
      601 gcggcggctt ccagatgcga taggccgtca aaggcggtga gcacgagagg agccttcaga
      661 gcgtcaggaa gctttgcaat cgccagctcc nnnnnnnnnn ccttcatcga ttgctgttgg
      721 cgcaattgat catcggtcgc gaggcctgga tcaatcgctt gggcgagcgc atcgtccggc
      781 gctgcggctc cgaaaaagaa ccggcgcacg gccatcttgc gcccgcggtc tcgcgccttg
      841 ttgatcgcga tggtgcgcag ccagggggca aaggcgcggg aggggtcgta ttttcccagt
      901 gcggcccacg ctgcaacgaa cgcctcgtgc acggcctcgt aggcctcgtc ggcatcgccg
      961 gtgtagcgcc gcagcagtcg gtagagcgct tctttatgtc ggcccaccag taacgcgaag
     1021 gcgcgctcat cacccttcgc ggcttggcac gccagagcct cgtcggtggg cgtcgctgca
     1081 gtcactgcga ctgttcggtg aggttcttca cgaccgtgtc gtcaaagcgc gaggtctg
//
LOCUS       DAOMEO010001200         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12638_length_1137_cov_3.515712, whole genome shotgun sequence.
ACCESSION   DAOMEO010001200 DAOMEO010000000
VERSION     DAOMEO010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12638_length_1137_cov_3.515712"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLH31_19700"
     CDS             <1..245
                     /locus_tag="PLH31_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91452.1"
                     /translation="LAPSTPVRLAVAALFAAPAALAGYHATHGLVAMAVPTEGWRQAF
                     AILGGLVIGFTALARLSLPRGRVEPRAARELRMSRH"
     gene            867..>1137
                     /locus_tag="PLH31_19705"
     CDS             867..>1137
                     /locus_tag="PLH31_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HQR91453.1"
                     /translation="MATIGTFKKSGNELNGEIVTLSVQAKGVRIVPETNRSGDNAPSH
                     RVYVGRAEIGAAWSKTSNEGRDYLGLKLDDPSFTAPIYANLFDDED"
BASE COUNT          172 a          394 c          381 g          190 t
ORIGIN      
        1 ccttggcgcc gtcgaccccg gtgagactgg ccgtcgccgc tctattcgcg gctcccgccg
       61 ccctcgcggg ctaccatgcc acgcatggcc tggtcgcaat ggcagttccg acggaaggat
      121 ggcggcaagc ctttgcgatc ctcggcggac tcgtcatcgg gttcaccgcg ctggcccgcc
      181 tgagcctgcc tcgcggtcgg gtcgaaccca gggccgcgcg cgaattgcgc atgtcccggc
      241 actgatgggg ggctccggac gcgttgagcc gccagactgg ctgaagcggt gcaggcgctc
      301 tgggccgggc gttggggatg ggggaaacct gatctgcggt gcagggccat gctccagagt
      361 cggcaaagcg ggcggtgtcg tggaacgatc ctgcttgggc gctcgcgctc ccgggggatg
      421 ttggcggctc ggggcggcgc ttcaccggcc gaaccccggg taagaaggcg ggtcgggcag
      481 cgttcggcgc gttgacccgg cacgccgatt tgtacgctgg actggccgga acttctacgc
      541 cggacgtttc gccttgcttg gctgctcgga gcttcgccag gtctgtcgtc ggatcggctg
      601 tcaaagcacg ccccgtccgc ggtctcttga tgggctgatc tggccgaagg acggcttcgc
      661 ctcgagcgcc tcagccgcaa cgtcgttcgt ccgactttct tccccggccc tttgggcctc
      721 ctcgcgggac aagaaagtct cccgaccgac atcctccgct gcgctgcggc cctaacgggt
      781 gcgtcgtcgc tcgccttgcg gcccttcgat caccatcgag gccgcgatgg gcgcggtgct
      841 cgagacacca tccaaggaga acgaccatgg ccaccatcgg caccttcaag aagagcggca
      901 acgagctcaa cggcgagatc gtcaccctga gcgtccaggc caagggcgtc cgcatcgtgc
      961 ccgagaccaa ccgcagcggc gacaacgcgc ccagccaccg ggtctatgtc ggccgggccg
     1021 aaatcggcgc cgcctggagc aagacctcca acgagggccg cgactatctg ggcctcaagc
     1081 tggacgatcc cagcttcacc gctccgatct acgccaacct cttcgacgac gaggacg
//
LOCUS       DAOMEO010001201         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12654_length_1136_cov_2.738205, whole genome shotgun sequence.
ACCESSION   DAOMEO010001201 DAOMEO010000000
VERSION     DAOMEO010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_1136_cov_2.738205"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..944)
                     /locus_tag="PLH31_19710"
     CDS             complement(252..944)
                     /locus_tag="PLH31_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91454.1"
                     /translation="MKSACFDPFPFPDPSEALKARLRALGEELDATRKTVLADHADLT
                     LTGLYNLLEKVRAGTALTPIEQDQKTRGRVLILKELHDQIDAATADAYGWPRDLTDEQ
                     ILERLVALNAERAREEAAGHVRWLRPDYQIPRFAKGAAAKTSELDLGEAPAAGDDLPD
                     FPKDAADRPAAVLSVLDHAAAPLDVAAIARSFNKGGKRIEQAVVKALPALVRYGHVTA
                     LKDGRYAGRKAA"
BASE COUNT          174 a          365 c          394 g          203 t
ORIGIN      
        1 ggcgtcggtc ccggatcttc gcgctgcgcg ctcgtccggg atgacaaccg tttttaggcc
       61 atatgagaag cgccccccgg gaaaccgggg ggcgttttcc gtttgggggc cctccccttc
      121 tccccttgcg ggagaaggtg gctcgcgtca gcgagacgga tgaggggttg aaaaccctgt
      181 ccggcacggt ctgcgcgacc cctcatccgt cggctgcgcc gacaccttct cccgcagggg
      241 gagaaggacc ttcacgccgc cttgcgcccc gcatagcggc cgtctttcag ggcggtgacg
      301 tggccgtagc ggaccagggc cggcagggcc ttgaccacgg cctgttcgat gcgcttgccg
      361 cccttgttga acgaccgggc gatggcggcg acgtccagcg gggcggcggc gtggtccagc
      421 accgacagca cggcggcggg gcggtcggcg gcgtccttgg ggaagtcggg tagatcgtcg
      481 cccgccgccg gggcctcgcc cagatccagc tcgctggtct tggccgccgc ccccttggcg
      541 aagcgcggga tctgatagtc gggccgcagc cagcgcacgt gaccggccgc ctcctcgcgg
      601 gcccgctcgg cgttcagggc caccagccgc tccaggatct gctcatcggt caggtcgcgc
      661 ggccagccat aggcgtcggc cgtggcggcg tcgatctggt cgtgcagttc tttcaggatc
      721 agcacccggc cgcgcgtctt ctggtcctgc tcgatcggcg tcagggccgt gccggcccgg
      781 accttttcca gcaggttgta caggcccgtc agggtcaggt cggcatggtc ggccagcacg
      841 gtcttgcgcg tggcgtccag ctcctcgccc agggcgcgca ggcgggcctt gagggcttct
      901 gacgggtcgg gaaaggggaa cgggtcgaag caggcacttt tcacgtagac cggcctatct
      961 tcgagcaaac cgcctgacga gattgcccaa tgagtgtgaa cgcgagaaga gagtatcgct
     1021 agtatagcgg catcttcgct agcaacgacg atcaatttat tgtccggaag aatcgaaatg
     1081 tctagaaact gaaaaacacg atgcttagtc gtttcgacag tcgcgaggta gcggcc
//
LOCUS       DAOMEO010001202         1136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12655_length_1136_cov_2.654024, whole genome shotgun sequence.
ACCESSION   DAOMEO010001202 DAOMEO010000000
VERSION     DAOMEO010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12655_length_1136_cov_2.654024"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLH31_19715"
     CDS             complement(<1..372)
                     /locus_tag="PLH31_19715"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HQR91455.1"
                     /translation="MTDETLIPASPSEDDYVRQVTQATPLPLLPENDPFALFSEWLAE
                     AGKKEPNDPNAVAVSTVDADGMPDSRMVLLKDFDQRGFVFYTNTQSAKGVELNAHPKA
                     ALLFHWKSLRRQVRIRGLVEPV"
     gene            472..780
                     /locus_tag="PLH31_19720"
     CDS             472..780
                     /locus_tag="PLH31_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91456.1"
                     /translation="MRHLLVAAWIEMTGATIIAGTVLAIDVVRQRPFTGWTDAVLSLS
                     PSVMIGAFLVLLAGARLRRALAGLADPRPAPPTAWATAGVFIVAMAVLLALASALIRR
                     "
     gene            830..>1136
                     /locus_tag="PLH31_19725"
     CDS             830..>1136
                     /locus_tag="PLH31_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQR91457.1"
                     /translation="MSARPASSLTLSAARALLGVAPGAGERELRAAYREAAKRAHPDR
                     PGGDAALFREVLAAYRLLQDQPPPRAIHFPPACTRPAAEAAQATLEIDVATAVEGGAM
                     "
BASE COUNT          169 a          404 c          369 g          194 t
ORIGIN      
        1 gaccggctcg accaggccgc ggatccgcac ctggcggcgc agggatttcc agtgaaacag
       61 cagcgccgcc ttggggtggg cgttcagctc aacccccttg gcgctctggg tgttggtgta
      121 gaagacaaag ccgcgctgat cgaaatcctt cagcagcacc atgcgcgaat ccggcatgcc
      181 gtcggcgtcg accgtcgaca ccgccaccgc gttgggatcg ttgggctcct tcttgccggc
      241 ctcggccagc cactcggaaa acagcgcaaa cggatcgttt tccgggagaa ggggcaaggg
      301 cgtcgcctgc gtcacctggc ggacatagtc gtcctcgctg ggcgaggcgg ggatcagggt
      361 ttcgtcggtc atgggcggga tatagacccg ccccggccac gccgccacag cagcttgacg
      421 cgctttgccc gataatgcac ccataggtcg tatgagctag ggacatcccg catgagacat
      481 cttctggtcg cggcctggat cgagatgacc ggcgcgacga tcatcgctgg aaccgtgctg
      541 gcgatcgacg tcgtgcgcca gcgccccttc accggatgga cagatgcggt cctgtcattg
      601 tcgccgagcg tgatgatagg ggcctttttg gtcttgctcg ccggcgccag acttcgtcgg
      661 gcgctcgccg gcctggccga cccgcgaccc gcgccaccca ccgcctgggc gacagccggc
      721 gtcttcatcg tcgccatggc tgtgcttctc gccctggcgt cggccctgat ccggcgctga
      781 gagtcttaac ttaacccggc gttgaccaag tcccggcagg gtcgacggga tgtctgcacg
      841 gcccgcctct tccctgaccc tttccgctgc ccgcgccctg ctcggcgtgg cgcccggcgc
      901 cggcgagcgc gaactgcgcg cagcctatcg cgaagcggcc aagcgcgctc accccgaccg
      961 tcctggcggc gatgcggccc tgtttcgcga agtgctggcc gcctaccgtc tgctgcagga
     1021 ccagccgccg ccccgcgcga tccatttccc gccggcctgc accaggcccg ccgctgaagc
     1081 cgcacaggcg acgctggaaa tcgatgtcgc caccgccgtc gaaggcggcg ccatgg
//
LOCUS       DAOMEO010001203         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12676_length_1135_cov_2.311111, whole genome shotgun sequence.
ACCESSION   DAOMEO010001203 DAOMEO010000000
VERSION     DAOMEO010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12676_length_1135_cov_2.311111"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLH31_19730"
     CDS             <1..656
                     /locus_tag="PLH31_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR91458.1"
                     /translation="AALLAAACDAGPGERVIEAGCGVGGALLSAAARRPDSRFHGVER
                     DPQAAELARGNAALNDMANRVEITTGDIEAGFRALDLPVFDAAISNPPFFDDPGALRA
                     PAPAKTGAWMADGGLAAWSGFLLKAVREGGTITIIHRADRLADILALLAPKAGSFRIR
                     PVQPFADAPAKRVIVRAIKTGKAPLVLLPALVLHERDGGQHSAAAEAILRGETDLGW"
     gene            complement(781..1135)
                     /locus_tag="PLH31_19735"
                     /pseudo
     CDS             complement(781..1135)
                     /locus_tag="PLH31_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518582.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
BASE COUNT          158 a          402 c          407 g          168 t
ORIGIN      
        1 atgcggcgct gctggcggcg gcctgcgacg ccgggccggg cgagcgggtg atcgaggcgg
       61 gctgcggcgt cggcggcgcg ctgctgtcgg cggcggcccg tcgtccagac agccgattcc
      121 acggggtgga acgcgatccg caggccgccg agctggcgcg cggcaatgca gccttgaacg
      181 acatggccaa ccgcgtcgaa atcaccaccg gcgacatcga ggccggcttt cgcgccctgg
      241 acctgccggt gttcgacgcg gcgatcagca atccgccgtt cttcgacgat cctggcgcgt
      301 tgcgagcccc cgccccggcc aagaccggcg cctggatggc cgacggcggg ctggcagcct
      361 ggtccggctt cctgctcaag gccgtgcgcg agggtgggac catcacgatc atccaccgcg
      421 ccgaccgcct ggccgacatc ctggccctgc tggcgcccaa ggccgggtcg ttccggatcc
      481 ggccagtcca gcccttcgcc gacgccccgg ccaagcgggt gatcgtgcgg gcgatcaaga
      541 cgggcaaggc gccgctggtg ctgctgccgg ctctggtgct gcacgagcgg gatgggggtc
      601 agcactcggc ggcggcggag gcgattctca ggggcgagac ggatctgggg tggtgaaacc
      661 ctccccctga agggggaggc ggccggaggc cggtggggga agaccgcgcc gagttcgcag
      721 cacttccccc tccgtcgcct gcggcgacac ctcccccgcc agggggagga tctgtgaacg
      781 ctaggttccc acgacaaagc tcaccgtggt ggtcgtcgag ccgccgatat tcagcgtctg
      841 caccgtcttc gccccctcga cctgatagtc gccagccgtg cccgtcacct gcttgaaagc
      901 gtccagcgtc atccgtacgc ccgtcgcgcc cacagggtgc cccgcgccga tcaggccgcc
      961 agagggattg atcggcagcc gaccgcccat ggcgatgtct ccggcctcga cagccttcca
     1021 gctgtcgccg ggcgcggtca ggcccagatg gtcgatggcc atgtattcgg tggcggtgaa
     1081 tcagtcgtgg gtttcgaccg cgtcgaggcc cgagacatcc ggcgcgatcc cggcc
//
LOCUS       DAOMEO010001204         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12694_length_1134_cov_2.074143, whole genome shotgun sequence.
ACCESSION   DAOMEO010001204 DAOMEO010000000
VERSION     DAOMEO010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12694_length_1134_cov_2.074143"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLH31_19740"
     CDS             complement(<1..182)
                     /locus_tag="PLH31_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91459.1"
                     /translation="MRRLISGLSLAALLASPAAAQDLLNALFSDHAVVQRDRPLPVFG
                     TATPGEAVTVAFAGKTA"
     gene            complement(322..>385)
                     /locus_tag="PLH31_19745"
     CDS             complement(322..>385)
                     /locus_tag="PLH31_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91460.1"
                     /translation="FEIMVGPNSVDLKKATLTVV"
     assembly_gap    386..395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<396..>1134)
                     /locus_tag="PLH31_19750"
     CDS             complement(<396..>1134)
                     /locus_tag="PLH31_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQR91461.1"
                     /translation="EAVEVAKNADVVVMVLGDNEQTSREAWADNHLGDRNDLDLIGQQ
                     NDLARAIFDLGKPTVVFLLNGRPLSINLLAERADAIIEGWYLGEQTGTAGADILFGRA
                     NPGGKLPVSIARNVGQLPVFYNRKPTSRRGYLFDEKTTPLYPFGFGLSYTTFDISAPR
                     LAKASIAQGETVKVEVDVTNTGKMAGDEVVQLYVRDVAASVTRPLLELKHFKRVTVAA
                     GAKTTVTFEIAPSDLWFWNLEMKRVVEP"
BASE COUNT          180 a          365 c          392 g          187 t
ORIGIN      
        1 gcggtcttgc cggcaaaggc gacggtgacg gcctcgcccg gcgtggcggt tccgaacacc
       61 ggcagcggcc ggtcgcgctg gaccaccgca tggtcagaga acagggcgtt cagcaggtcc
      121 tgggcggcgg cgggactggc cagcagggcg gccagactca ggcccgagat caggcgacgc
      181 atggtgttac tccctccgaa tagatcctcc ccctctgggg gaggtggccc agggggccgg
      241 agggggccag cacggccttg gccgagatga ccccctcagt cgctccgcga cagctccccc
      301 agaggggagc agcttgccga cctagaccac cgtcagcgtg gccttcttca ggtccaccga
      361 gttgggtccg accatgatct cgaacnnnnn nnnnncggct cgaccacgcg cttcatctcg
      421 aggttccaga accacaggtc cgatggcgcg atctcgaagg tgacggtggt cttggcgccg
      481 gccgcgacgg tgacgcgctt gaagtgcttc agctccagca gcggacgggt caccgaggcc
      541 gccacgtcgc ggacatagag ctgcaccacc tcgtcaccgg ccatcttgcc ggtgttggtc
      601 acatcgacct cgaccttgac cgtctcgccc tgggcgatgc tggccttggc caggcgcggg
      661 gccgagatgt cgaaggtggt gtaggacagg ccaaagccga acggatagag cggtgtggtc
      721 ttttcgtcga acagatagcc ccggcgcgag gtgggcttgc ggttgtagaa gaccggcagc
      781 tggccgacat tgcgggcgat gctgacgggc agcttgccgc ccggattggc gcggccaaac
      841 aggatgtcgg cgccggccgt tccggtctgc tcgcccaggt accagccctc gatgatggcg
      901 tcggcgcgct cggccaggag gttgatcgac agcggacggc cgttgagcag gaacaccacg
      961 gtgggcttgc ccaggtcgaa gatcgccctg gccagatcgt tctgctgacc gatcaaatcg
     1021 aggtcgttgc ggtcgcccag gtggttgtcg gcccaggcct cgcggctggt ctgctcgttg
     1081 tcgcccagca ccatgaccac cacgtcagcg ttcttggcca cctcgacggc ttcg
//
LOCUS       DAOMEO010001205         1133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12716_length_1133_cov_1.778293, whole genome shotgun sequence.
ACCESSION   DAOMEO010001205 DAOMEO010000000
VERSION     DAOMEO010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12716_length_1133_cov_1.778293"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLH31_19755"
     CDS             complement(<1..424)
                     /locus_tag="PLH31_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR91462.1"
                     /translation="MSPSDEARPATPVRTSFIAAYTLAQIGAFVSFMPLLQVLLPLKA
                     EAIDPANKALLLSQVAFYGALVASVANLLAGAISDRTTSRFGRRRPWMVVGALGAAGA
                     YLLIMAARTPMALIGGIVLFQLMFNLMFSALVAVLPDRV"
     gene            631..>1133
                     /locus_tag="PLH31_19760"
     CDS             631..>1133
                     /locus_tag="PLH31_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR91463.1"
                     /translation="MASVTIYDVAARAGVSIKTVSRVMNKEPNVRPAMRDRVLEAANA
                     LDYSPNLSARSLAGSRSFVIAVFVDAALTIDHWKSERGADYLSRIQLGATLQCRDAGY
                     HLLIELIDHDAPRVRQEVAALLAALKPDGVILTPPSSDNETVLGLLDKAGTPYVRLGP
                     ERPQGLGP"
BASE COUNT          208 a          396 c          362 g          167 t
ORIGIN      
        1 gcacacggtc gggcaacacg gccaccaagg ccgagaacat caggttgaac atcagctgga
       61 acagaacaat gccgccgatc agggccatcg gcgtcctggc ggccatgatc agcagatagg
      121 cgccggccgc gcccagcgca ccgaccacca tccaggggcg acgccgacca aagcgcgagg
      181 tggtgcgatc gctgatggcc ccggccagca ggttggcgac actggccacc agggcgccat
      241 agaaagccac ctggctgagc aacagcgcct tgtttgccgg atcgatggct tcggccttca
      301 ggggcagcag cacctgcaac agcggcataa agctgacgaa ggccccgatc tgggccaggg
      361 tataggcggc gatgaaactg gtcctgaccg gcgtggccgg ccgggcttcg tcgctcggcg
      421 acatcgtctc tcccctcgcg cggtctgcgc cgcgtctggg cggagactgg ccctccggat
      481 cagccgacac aaggccgccg acgccgcatt cgaccgcccc acgccaactg cgagcaaatt
      541 tttctcgctg caagcgtcgc tagagccgca acaaatgctc taggctgcgc tcatgggttt
      601 gagtctcatc cagcaaggca tggcaggccg atggcctcgg tgacgatcta cgacgtcgcc
      661 gcgcgagccg gcgtctccat caagaccgtt tcccgggtca tgaacaaaga gcccaatgtg
      721 cggcccgcca tgcgcgaccg cgtgctcgag gccgccaacg ccctggacta cagccccaac
      781 ctgtcggccc gcagcctggc gggatcacgc tcgttcgtga tcgccgtgtt cgtggacgcc
      841 gccctgacca tcgatcactg gaaaagcgag cgcggcgccg actatctgag ccgtattcag
      901 ctgggcgcga cgctccagtg ccgcgacgcc ggctatcacc tgctgatcga actgatcgat
      961 cacgacgcgc cgcgggtccg tcaggaggtc gccgccctgc tggcggccct gaagccggac
     1021 ggcgtcatcc tgaccccgcc aagctcggac aacgagacgg ttctgggact gctcgacaag
     1081 gcggggacgc cctatgtgcg cctgggtccc gagcgccccc agggccttgg gcc
//
LOCUS       DAOMEO010001206         1132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12739_length_1132_cov_1.810585, whole genome shotgun sequence.
ACCESSION   DAOMEO010001206 DAOMEO010000000
VERSION     DAOMEO010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12739_length_1132_cov_1.810585"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLH31_19765"
     CDS             complement(<1..633)
                     /locus_tag="PLH31_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012521160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQR91464.1"
                     /translation="MAEIFAFFRLFFDSEMLNPHGICLLWRPELLITHAVSDFLIGSA
                     YFSIPIALAVFLRQRPDVKFSWVVWMFIAFILLCGVTHFMMVWTLWNADYGIEALLKA
                     ATAAVSLVTAIALWPLLPKVVAMPSTALLQARIEERDAALAEARAAMNAMVEMREHER
                     RQSLLLNELNHRVKNTLAAVQSIAIQTLNTATSLEAGRDLFVDRLISLSAT"
     gene            800..>1132
                     /locus_tag="PLH31_19770"
     CDS             800..>1132
                     /locus_tag="PLH31_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQR91465.1"
                     /translation="MNRRELLALAGALGLAPSLASAQDADPAATARDAWLYALPLIEM
                     AAARARILSAPGAAVNVLRHDRELADHTKRAVTTPNNDTLYSTAFVDLTQGPVILTIP
                     AMGTRYYSV"
BASE COUNT          212 a          378 c          373 g          169 t
ORIGIN      
        1 ggtggccgag agcgagatca ggcggtccac gaaaagatcg cggcccgcct ccagcgaggt
       61 ggcggtgttc agggtctgga tcgcgatcga ctgcacggcg gccagggtgt tcttcacgcg
      121 gtggttcagc tcgttgagca gcagcgactg ccgccgctcg tgctcgcgca tctcgaccat
      181 ggcgttcatc gccgcgcggg cttcggcgag ggcggcgtcg cgctcttcga tccgcgcctg
      241 cagcagggcg gtcgaaggca tggcgaccac cttgggcagc aggggccaca gcgcgatcgc
      301 ggtgaccagc gagaccgccg ccgtcgcggc cttgaggagc gcctcgatcc cgtagtccgc
      361 gttccacagc gtccagacca tcatgaaatg cgtgacgccg cacagcagga tgaaggcgat
      421 gaacatccac accacccagc tgaatttcac gtccggtcgc tggcgcagga acacagccag
      481 ggcgatgggg atcgagaagt aggcgctgcc gatcaggaag tcggatacgg cgtgggtgat
      541 cagcagttcg ggacgccaca gcaggcagat gccatgcgga ttgagcatct cggagtcgaa
      601 aaacaggcgg aaaaacgcga aaatctcggc catgcccggc tcccaaggtc gacgccaacg
      661 ccttcccgca acgtgcgact cgtcgaaggt gatccattcc cgcgcgtgga acaatggcca
      721 agaccagggc cgcaatagcc gagacagggc ctcctgcgcc gcgctaacct gcctcaaaca
      781 catgttgggg cgaaacgtca tgaaccgtag agaactgctg gccctggctg gcgcgctggg
      841 tcttgcgccg tcgctggcga gcgcccagga cgccgatccg gcggccaccg cgcgcgacgc
      901 ctggctctac gccctgccgc tgatcgagat ggccgccgcg cgggcgcgga tcctctcggc
      961 tcccggggcg gcggtcaatg ttctgcgtca tgaccgcgag ctggccgacc acaccaagcg
     1021 cgcggtgacg acgcccaaca acgacacgct ctattcgacg gccttcgtcg acctgaccca
     1081 ggggccagtg atcctgacca ttcccgccat gggaacccgc tactactccg tc
//
LOCUS       DAOMEO010001207         1131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12749_length_1131_cov_2.554833, whole genome shotgun sequence.
ACCESSION   DAOMEO010001207 DAOMEO010000000
VERSION     DAOMEO010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12749_length_1131_cov_2.554833"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..>1131)
                     /locus_tag="PLH31_19775"
     CDS             complement(233..>1131)
                     /locus_tag="PLH31_19775"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR91466.1"
                     /translation="GGGGSVGRGGGPAAEAVMAQPAGTVQGRIRMTEQGEMIARRFGD
                     QPTARRNLDGLAAAVLMASDRPVVRPDPKVEAAMTALAQASFEGYRALVYDDPAFEDF
                     FWSATPIGEIVGLNIGSRPASRTASRKIEDLRAIPWVFSWSQSRFMLPGWYGFAAGVE
                     RAGLSVQQLQDLAGGFDFFASLLSNMELALAQSHMDIAARYVALSSDPANATRIFETI
                     KVEHEAARRLALTIRGGTSLLDNQPELAESVALAGLSVDPLNHLQLELLSRRRGGDQD
                     EAVRLAIQLTVAGIAAGLRNTG"
BASE COUNT          203 a          387 c          376 g          165 t
ORIGIN      
        1 ggctcgacgc cccgaaagtt tgcgcaggag cgagctcaaa agagccgcaa cgagacggcc
       61 cgaccaagtc gttccgaacc gtaatgatac cggtgtcatc atgaggtgag cccgtggcgc
      121 caccggtgtc atcgcgcaga taatcagaaa ccgacggttt aggcaagcgg tctgcagtac
      181 ttgaaaggct cagctaggcc gttgaaatca tgaagaattc aacgattgat gattaaccgg
      241 tgttgcgcag gcccgcggcg atcccggcca cggtcagctg aatcgccagc cgaaccgcct
      301 catcctggtc tccgccccgc cgccgagaca gcagttccag ctgcaggtgg ttgagcggat
      361 ccaccgaaag gccggccagg gcgacggact cggccagctc cggctggttg tccagcaggc
      421 tggttccacc ccggatggtc agggccagac gccgggcggc ctcatgctcg accttgatgg
      481 tctcgaagat ccgggtcgca ttggccgggt cggacgacag cgccacatag cgtgcggcga
      541 tgtccatgtg gctctgggcc agggccagct ccatgttcga cagcagggag gcgaagaagt
      601 cgaagccgcc ggccaggtcc tgcagttgct gcaccgacag ccctgcccgc tcgaccccgg
      661 cggcgaagcc gtaccagccg ggcaacatga accgcgactg cgaccagctg aacacccacg
      721 gaatggcccg caggtcctcg atcttgcggc tggcggtccg gctggcgggg cgcgacccga
      781 tgttgaggcc gacgatctcg ccgatcggcg tggccgacca gaagaagtcc tcgaaggccg
      841 gatcgtcata gaccagcgcc cgatagccct cgaacgacgc ctgggccagg gcggtcatcg
      901 ccgcctcgac cttcggatcg ggacgcacga cgggccggtc gctggccatc agcacggccg
      961 cggccaggcc atccagattg cggcgcgcgg taggctggtc gccaaagcgc cgggcgatca
     1021 tctcgccctg ctcggtcatt cggatgcgcc cctgaacggt gccggccggc tgggccatca
     1081 ccgcctcggc ggcggggccc ccgccccggc cgacgctgcc gcccccgccg t
//
LOCUS       DAOMEO010001208         1131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12756_length_1131_cov_2.087361, whole genome shotgun sequence.
ACCESSION   DAOMEO010001208 DAOMEO010000000
VERSION     DAOMEO010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12756_length_1131_cov_2.087361"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLH31_19780"
     CDS             complement(<1..511)
                     /locus_tag="PLH31_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR91467.1"
                     /translation="MQDANQNGIRKIVIVGGGSAGWICAAMASHYFHKSGVEVELIES
                     EEIGTIGVGESTIPQLIRSLGIDEQDFIQKTQASFKLAIRFENWRKKGETYYHPFGQI
                     GGPIGRHEFYQCWLRAKANGHPSNLQDFAPATVMADQWKFTPPFRAQKTLLSGASYAL
                     HIDAKLVAQY"
     gene            complement(536..>1131)
                     /locus_tag="PLH31_19785"
     CDS             complement(536..>1131)
                     /locus_tag="PLH31_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR91468.1"
                     /translation="HEPRQIRFTPGRRLKAWNKNCVALGLAAGFLEPLESTSIALIET
                     GIEKIKQLFPDRTFNQGVLDEFNDMSKLEMERVRDFIILHYKANQRNDTAFWRDCQAV
                     EIPDTLKHKIELFRERGHFIRYRWEMFQPASWLAIYTGFDILPRTWDPSIEGLDIESL
                     RRGMTEMRKAVKAAVDAAPTHGDFLDKYARPAPLAAQ"
BASE COUNT          174 a          344 c          372 g          241 t
ORIGIN      
        1 ggtattgggc caccagcttg gcgtcgatgt gcagggcgta gctggcgccg ctgagcaggg
       61 tcttctgggc gcggaacggc ggcgtgaatt tccactggtc ggccatcacc gtggccggag
      121 caaagtcctg caggttagag ggatggccat tggccttagc gcgcagccag cactggtaga
      181 actcgtgccg accaatgggc ccgccgatct ggccgaaggg gtggtagtag gtctcgccct
      241 ttttccgcca gttctcgaaa cggatcgcga gtttgaagct ggcctgggtc ttctggatga
      301 agtcctgctc gtcgatgccc aggctgcgga tcagctgcgg gatggtcgac tcgcccacgc
      361 cgatggtgcc gatctcctcg gactcgatca gctcgacctc gacgccgctc ttgtggaaat
      421 agtggctggc catggccgcg cagatccagc cggcgctgcc gcccccgacg atcacgatct
      481 tgcggatccc gttctgattg gcgtcctgca tgtccgtccc tgtcttttct tgtctttatt
      541 gcgcagcgag cggcgcgggc cgcgcgtatt tgtcgaggaa gtcgccatgg gttggcgcgg
      601 cgtcgaccgc cgccttcacg gccttgcgca tctcggtcat gccccggcgc agcgactcga
      661 tgtccaaccc ttctatgctg gggtcccagg tcctgggcag gatgtcgaag cccgtataga
      721 tcgccagcca gctggccggc tggaacattt cccagcgata gcggatgaag tggccgcgct
      781 cgcggaacag ctcgatcttg tgcttgaggg tgtcgggaat ctccacggcc tgacagtcgc
      841 gccagaaggc cgtgtcgttc ctctggttgg ccttgtagtg caggatgatg aagtcgcgga
      901 cgcgctccat ctccagcttc gacatgtcgt tgaactcgtc gagcacgccc tgattgaagg
      961 tgcggtccgg gaagagctgc ttgatcttct cgatgccggt ttcaatcagg gcgatgctcg
     1021 tgctctccag cggctcgagg aatcccgccg ccaggcccag ggcgacgcag ttcttgttcc
     1081 aggccttcaa acggcggccc ggggtaaagc ggatctgacg cggctcgtgc a
//
LOCUS       DAOMEO010001209         1130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12772_length_1130_cov_2.309767, whole genome shotgun sequence.
ACCESSION   DAOMEO010001209 DAOMEO010000000
VERSION     DAOMEO010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_1130_cov_2.309767"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLH31_19790"
     CDS             <1..583
                     /locus_tag="PLH31_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91469.1"
                     /translation="PLAGGPVPGQVTPPVQVAQVAPVARPQIQTLAEPRKRLAASTVK
                     TAKAPPPLLGLARSSERPIRLTKVEDIEIQPFAPPGQQTLFKRLSVDASALMGKISTS
                     AKDAQKGRWTLFAASSGKALSMNVVRDTVNGWRGAGLSQDRLAGFGTHQVGLGWRKDG
                     KQISLSATRRKLRAQDYSERDIVYGVSLSIKPR"
     gene            complement(618..914)
                     /locus_tag="PLH31_19795"
     CDS             complement(618..914)
                     /locus_tag="PLH31_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQR91470.1"
                     /translation="MSATHLDDLKQQMEAAVAAQDFETAGKLRDQIESVMHGNMVRGV
                     EPTAQASYFQRQVPGKMGLGTDQQVYAPPPGWTPPKKPDPMTAGHSRGARRRKS"
     gene            complement(970..>1130)
                     /locus_tag="PLH31_19800"
     CDS             complement(970..>1130)
                     /locus_tag="PLH31_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HQR91471.1"
                     /translation="AGGSVVGAWVATKLDRLAQTLGEVIDQSITEQRPANLVADEIAR
                     ARIAAARG"
BASE COUNT          176 a          403 c          372 g          179 t
ORIGIN      
        1 accgctggcc ggcggtcccg tcccgggcca ggttacgccg ccagttcagg tggcccaggt
       61 cgccccggtc gccaggcccc agatccagac cctggccgaa ccccgcaagc gcctcgccgc
      121 cagcaccgtg aagacagcca aggcgccccc gccgcttctg ggtctggcgc ggtcgtctga
      181 gcggccgatc cggctcacca aggttgaaga tatcgagatc cagcccttcg ccccgcccgg
      241 tcagcagacg ttgttcaagc gcctgtcggt cgacgcctcg gcgctgatgg gcaagatcag
      301 cacctcggcc aaggacgccc aaaaggggcg ctggaccctg ttcgccgcca gttcgggcaa
      361 ggcgctcagc atgaatgtcg ttcgcgacac cgtgaacggc tggcgcgggg ccggtctgtc
      421 gcaggatcgt ctggccggct ttggcaccca ccaggtcggc ctgggctggc gcaaggacgg
      481 caagcaaatc tcgctcagcg ccacgcggcg caagctgcgc gcccaggact attccgagcg
      541 cgacatcgtc tacggcgtca gcctgtccat caagcctcgc tgagatctgg cccggacgcc
      601 cgggccgaaa gcctggatca agatttgcga cgccgcgctc cgcgcgaatg gccggcggtc
      661 atcgggtcgg gcttcttggg cggcgtccat cccggaggcg gcgcatagac ctgctgatcc
      721 gtgcccaggc ccatcttccc cggaacctga cgctggaaat acgaggcctg ggctgtcggt
      781 tcgacgccgc gcaccatgtt gccgtgcatg acgctctcga tctggtcgcg cagcttgccg
      841 gccgtctcga agtcctgagc ggcgacggcc gcctccatct gttgcttcag atcgtcaagg
      901 tgggtggcgg acatggcgcg gctccctggc tggatggtcc agaagagaac gcgcaaacgc
      961 ctcgaaaggc tatccccgcg ccgcagcgat ccgcgcccgg gcgatctcgt cggccaccag
     1021 attggcggga cgctgctcgg tgatggactg gtcgatgacc tcgcccagcg tctgggccag
     1081 gcgatccagc ttggtcgcca cccaggcgcc gaccactgac ccgccggctt
//
LOCUS       DAOMEO010001210         1129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12783_length_1129_cov_2.536313, whole genome shotgun sequence.
ACCESSION   DAOMEO010001210 DAOMEO010000000
VERSION     DAOMEO010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12783_length_1129_cov_2.536313"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..962
                     /locus_tag="PLH31_19805"
                     /pseudo
     CDS             168..962
                     /locus_tag="PLH31_19805"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            1047..1129
                     /locus_tag="PLH31_19810"
                     /pseudo
     CDS             1047..1129
                     /locus_tag="PLH31_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          192 a          385 c          369 g          183 t
ORIGIN      
        1 cgctacgctg ctgcgaccgt ttgtctcact cgtgggggcg cgtcgcgcat gacacggtaa
       61 aggaccccta cgccgcagag ttgagcgtcg agatcaaaat gtcctccgac gacaacgctc
      121 tggccgacgt aaggacgacc atggcccgag ccggcttttc gtcggccatg gccgacgcag
      181 tgccaatcct gatcagctat gtggaccgcg agcagcgcta tcgcttcgtc aatcgcgcct
      241 atgagcgctg gttcgagacc gaccgctcgc agatcgaagg gcgaaccctg ctcgaggtgc
      301 tcgggccgct ggcctatgag cgtcttcgcg atcatgtgac ccgcgccctg gccggagaaa
      361 gcctgatctt cgagggcgaa attccctatc gcagcgccgg catgcgtcat gtcgaagccc
      421 gctatgcgcc cgatgtggcg cccgacggct cgatcgccgg ctactatgtg ctggtcaccg
      481 acatctcggc ccgcaaaagt gtcgaggcgg aagtcgcgcg actgctcgac agcgagcgcc
      541 gccgcgtcgc cctgctggat ctgggccgca tgctgcgcga ggaagccgat ccagtcgcgg
      601 cagccaaccg ggcgtgcgaa ctgatggccc gccagctggg cgcggcccgg gccggctatg
      661 ccgaggttca gaccgacggg atatcgtcgc tgcacatcgg tgactgggtc gccgccggcg
      721 cgccgagcct gcttggaagc ctgctcgacc tcgatgcctt cggcccgcag atggcggctg
      781 agctgcgcgc ggggcgacag gtggtcatcc cggacgtgaa gcaggacccc cgcaccgacc
      841 accccgtggt cgtaaaggcc tatgaggcct tgcacatcca gagcttcgtc accgcgccgc
      901 tgatcaggga ccggcggatg gcggcctata tctatctggc ccaagacacc gcccgcgcct
      961 ggaccgacca cgagatcgcc ttcgtcgctg acgtggccga gctgatctgg atcgcggtgg
     1021 agcgcgcccg ctccgaagag gccttgaagc gggccgagga aaccgagcgc ctgctgatcc
     1081 gcgaagtcga ccaccgcgcc aagaatgtcc tgaccgtcgt ccagtccct
//
LOCUS       DAOMEO010001211         1128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12796_length_1128_cov_2.798695, whole genome shotgun sequence.
ACCESSION   DAOMEO010001211 DAOMEO010000000
VERSION     DAOMEO010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_1128_cov_2.798695"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLH31_19815"
     CDS             <1..967
                     /locus_tag="PLH31_19815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQR91472.1"
                     /translation="TLGMCEIMIQTLLDWQADPEIDLVMIDHTGEKAFCAGGDIRMLA
                     ESGAGDGVAARRFFHTEYRLNHLMFHYDRPIVAIMDGVVMGGGVGISMPAHYRIATER
                     TTFAMPETGIGLFPDVGGGWYLPRLPGKAGLWLALTGARIKGADCLRLGIATHFVEFG
                     AVEGLKKAIVADHRRIDETLKMFRADAGKAPLASIEQDLNRLFVGDSVEQIFEFLEMD
                     SSDWGKTQLAALKTKSPQTLKVAFRQLELGAAAEDFAEHMAMEYRIGARVVQKHDFIE
                     GVRAVIVDKDNAPKWSPPTLEGVTEAMLDEIFAPLAAEDEWTPLT"
BASE COUNT          197 a          371 c          379 g          181 t
ORIGIN      
        1 gaccctgggc atgtgcgaga tcatgatcca gaccctgctg gactggcagg ccgatcccga
       61 gatcgatctg gtgatgatcg accacaccgg cgagaaagcc ttctgcgccg gcggcgacat
      121 ccgcatgctg gccgagagcg gggcgggcga cggcgtcgcg gcgcgccggt tctttcacac
      181 cgaataccgg ctcaaccacc tgatgttcca ctacgaccgc cccatcgtgg cgatcatgga
      241 cggggtggtg atgggcggcg gcgtcgggat ctcgatgccg gcccactacc ggatcgccac
      301 cgagcgcacg accttcgcca tgccggaaac cggcatcggc cttttccccg atgtgggcgg
      361 cggctggtac ctgccgcgcc tgccgggcaa ggcggggctg tggctggccc tgaccggggc
      421 gcggatcaag ggcgccgact gcctgcgcct gggcatcgcc acccattttg tcgagttcgg
      481 cgctgtcgag ggcctgaaga aggcgatcgt cgccgatcac cgtcgcatcg acgagacgtt
      541 gaagatgttc cgcgccgacg ccggcaaggc cccgctggcc agtatcgagc aggatctcaa
      601 ccgcctgttc gtcggcgaca gcgtcgagca gatcttcgag ttcctggaaa tggattccag
      661 cgactggggc aagacccagc tggccgccct gaagaccaag tcgccccaga ccctgaaagt
      721 ggccttccgg caactggagc ttggcgcagc ggccgaggac ttcgccgagc acatggccat
      781 ggagtaccgg atcggcgccc gcgtggttca gaagcacgac ttcattgagg gcgtccgcgc
      841 ggtgatcgtc gacaaggaca atgcgcccaa atggtcgccg ccgactctcg aaggtgtgac
      901 cgaagcgatg ctcgacgaga ttttcgcgcc gctggcggcc gaagatgaat ggacgccgct
      961 gacgtaaggg atgtcgtagg ctaacctagg cgcaagaaga acactgccgg tcggaaggaa
     1021 tgccatgcgt aagccgcttc tcagtctcgt cgccgtgctc gggctcggcg cgtgcgccac
     1081 ggcgcccatg ggaccgccac cgccgcctcc gccgccctcg ggccctgt
//
LOCUS       DAOMEO010001212         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12810_length_1127_cov_2.672575, whole genome shotgun sequence.
ACCESSION   DAOMEO010001212 DAOMEO010000000
VERSION     DAOMEO010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_1127_cov_2.672575"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLH31_19820"
     CDS             <1..1077
                     /locus_tag="PLH31_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR91473.1"
                     /translation="ASKAAQAQSRMKRLAKMEPVATTIEERVAPFTLPSPPRPLPPPL
                     IRLERANVGYEEGRSILKNLNLRMDLDDRIGLLGVNGAGKSTFAKMIAGALGVQSGEF
                     HRDRKMRVGWFHQHQIEAMDPVDTPLEIIRRAMPDASESSRRSKLAQFGLGFEKQETT
                     VANLSGGERARLLLNMVAMDAPHMLILDEPTNHLDIDSRRALLDALNEYEGAVILITH
                     DRSLMELVADRLWLAADGTVKPFDGDMDDYAKFVLDRAKQAVKPTQVAREPAKAEATA
                     AAPKKTVAIEPLKRKIDAAEQVVTRTTRQVAELEAQLADPQLYKNPTKVAELTKRRDA
                     AQAKLDEAEATWMGLAEELAAAEA"
BASE COUNT          210 a          397 c          363 g          157 t
ORIGIN      
        1 gcctccaagg ccgcccaggc tcagtcgcgc atgaagcgtc tggccaagat ggagccggta
       61 gccacgacga tcgaggagcg cgtcgcgccc ttcaccctgc cctcgccgcc gcgccccctg
      121 ccgccgcccc tgatccgcct ggagcgggcc aatgtcggct atgaagaggg tcgctcgatc
      181 ctcaagaacc tcaacctgcg catggacctg gacgaccgca tcggtctgct gggcgtcaat
      241 ggcgccggca agtcgacctt cgccaagatg atcgccggcg ccctgggcgt gcagtcgggc
      301 gaattccatc gcgaccgcaa gatgcgggtc ggctggttcc accagcacca gatcgaggcg
      361 atggaccccg tcgacacccc gcttgagatc atccgccggg ccatgccgga cgcctcggaa
      421 tcctcgcgcc gctcgaaact ggcgcagttt ggtctgggct tcgagaagca ggagactacg
      481 gtcgccaacc tgtcgggcgg cgagcgcgcg cgcctgctgc tgaacatggt ggcgatggac
      541 gcgccgcaca tgctgatcct cgacgagccg accaaccacc tggacatcga cagccgccgc
      601 gccctgctgg acgctctgaa cgagtatgag ggcgcggtga tcctgatcac ccacgaccgc
      661 tcgctgatgg agctggtcgc cgaccgcctg tggctggccg ccgacgggac cgtgaagccc
      721 ttcgacggcg acatggacga ctatgccaag ttcgtgctgg accgcgccaa gcaggccgtg
      781 aagcccaccc aggtggcccg cgagccggcc aaggcggaag caaccgccgc cgcgccgaag
      841 aagacggtcg ccatcgagcc gctgaagcgc aagatcgacg ccgccgagca ggtggtcact
      901 cgcaccaccc gccaggtggc tgaactggaa gcccagctgg ctgatccgca gctctacaag
      961 aacccgacca aggtggccga actgaccaag cgacgcgacg cggcccaggc caagctcgac
     1021 gaagccgagg cgacgtggat gggccttgcc gaagagctgg cggctgcgga ggcctagggc
     1081 caatcgacat tcagccgacc attgtccttt attcaccgtc attcccg
//
LOCUS       DAOMEO010001213         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12815_length_1127_cov_2.457090, whole genome shotgun sequence.
ACCESSION   DAOMEO010001213 DAOMEO010000000
VERSION     DAOMEO010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12815_length_1127_cov_2.457090"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..266
                     /locus_tag="PLH31_19825"
     CDS             3..266
                     /locus_tag="PLH31_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003167295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HQR91474.1"
                     /translation="MPRTIKKPELRQMVPLADSTIWEMEQRGEFPKRFRLTPRCVVWD
                     LSEVQAWLEKRRRTPVTAAKAPDVRLRRTRPVHDGPGAARPHL"
     gene            complement(525..1061)
                     /locus_tag="PLH31_19830"
     CDS             complement(525..1061)
                     /locus_tag="PLH31_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91475.1"
                     /translation="MAEPYFPPLEVAGQTFAFDHLEPFVLEMATQSRPNGVKIDVRFS
                     NHCFSETFDAARHDDAVAVWDGPRRRVFCPIRYGLSQALPNILKGLPTAHVYQTPEAN
                     FLRIGVRNDGGAGDYRVFFRVKRGAGAGIDLKLFVESAYSPEPAQALAIAHMSKVRFA
                     VLIDKTLKGEKLKFHRKR"
BASE COUNT          193 a          371 c          371 g          192 t
ORIGIN      
        1 ccctgccgcg aacgatcaag aagccggaac tgcgccagat ggtgccgctc gcagacagca
       61 ccatctggga aatggagcag cgcggggagt tccccaaacg ctttcggctg acgccgcgat
      121 gcgtggtctg ggatctgtcg gaggtccagg cctggctgga gaaacgccgc cgcacccccg
      181 tcacggcggc caaggccccg gatgtgcgcc tgcgccgaac acgcccggtc cacgacggcc
      241 cgggcgcggc ccggccgcac ctgtgaggcg cgtgcgcttc agaaatcagc gagatggtca
      301 tagaatgctt catccgggga cggttctgac ccggagaccg ggacaggggc gtccagggct
      361 gatcattgat ttcttcaccc ccggggctta tatgtcctcc cgaggccaga tcggttgaaa
      421 cagcctcacc cgcctcttcg gaggtccacg cgtgagcgag atagggattt cggtcccgga
      481 agcggctacc gagtgacttc tgataagggc gcctccgggc gcccttatct tttccggtga
      541 aacttcagct tctcgccctt cagcgtcttg tcgatcagca cggcgaagcg aaccttgctc
      601 atgtgggcga tggcgagcgc ctgcgccggt tcagggctgt aggcgctttc cacgaagagc
      661 ttgagatcga tgccggcgcc ggcgccgcgc ttgacccgga agaagaccct gtagtcccct
      721 gctccgccgt cgttgcgcac gccgatccgc aggaagttcg cctccggcgt ctgatagacg
      781 tgagcggtcg gcaggccctt gaggatattc gggagcgctt gcgacagccc gtagcggatc
      841 ggacagaaga cgcgccgcct cgggccgtcc cagaccgcca ccgcgtcgtc gtgccgggcg
      901 gcgtcgaagg tttcgctgaa gcagtggttg ctgaaccgga catcgatctt cacgccgttc
      961 ggacgcgact gggtggccat ctccagcacg aagggctcga gatggtcgaa cgcgaacgtc
     1021 tgccccgcga cctccagcgg cggaaaatac ggctcggcca tggcctacag tcccaacagg
     1081 tccggcgacc acttgcgcag tccccggcgc atggccgtgc gcctggt
//
LOCUS       DAOMEO010001214         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12843_length_1125_cov_3.053271, whole genome shotgun sequence.
ACCESSION   DAOMEO010001214 DAOMEO010000000
VERSION     DAOMEO010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12843_length_1125_cov_3.053271"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="PLH31_19835"
     CDS             <1..>1125
                     /locus_tag="PLH31_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR91476.1"
                     /translation="ARLLSDPGKGGARAIALPDGRLLAAQGRSVQGLPTFREAFALSM
                     EDVGGQSAALRGQAANGALLDVAVQPLAEGALLAVAGSPSRNVANMDRQVMEGAFNLL
                     APLAVGIALALMLMMQSRKVEVAHREFVDSEQRFRLAVEAARCGIWEWDLRGDQVFLS
                     DVTGVMFGWGGGGIVSGQEMLDRISIDHRERVRQALANAAMYGAFDVSFRVPATQQGG
                     RSIWVDARGQGFGKPGEDGYVRIIGVALDVTEERLAQARAQAAENRLRDAIESVSEAF
                     VLWDRQGRLLMCNRNYRSVFNLEPKLLKPGAPRAEVNRFAALAIKQDHPAPDGAKGVR
                     EAELMDGRWIQISERRTAEGGLVMTAADITAIKNQEEARR"
BASE COUNT          167 a          404 c          391 g          163 t
ORIGIN      
        1 cggcccgtct gctcagcgat ccaggcaagg gcggggcccg cgcgatcgcc ctgcccgacg
       61 gtcgactgct ggcggcccag ggccgatcgg tacagggcct gcccaccttc cgcgaggcct
      121 tcgccctgtc gatggaagat gtcggcggcc aaagcgccgc cctgcgtggt caggccgcca
      181 acggcgccct gctggacgtg gcggtgcagc ccctggccga gggcgcactg ctggccgtcg
      241 cgggctcgcc gtcgcgcaac gtcgccaata tggaccgtca ggtcatggag ggcgccttca
      301 acctgctggc gcccctggcc gtcggcatcg ccctggcgct gatgctgatg atgcagagcc
      361 gcaaggtcga ggtcgcccac cgcgaattcg tcgacagcga gcagcgtttc cggctcgccg
      421 tcgaagccgc ccgctgcggc atctgggaat gggacctgcg cggcgaccag gtgttcctgt
      481 ctgacgtcac cggcgtgatg ttcggctggg gcggcggcgg catcgtctcg ggccaggaga
      541 tgctggaccg catctcgatc gatcaccgtg agcgcgtgcg ccaggccctg gccaacgccg
      601 ccatgtacgg cgccttcgac gtctctttcc gcgtacccgc cacccagcag ggcgggcgct
      661 cgatctgggt cgacgcccgg ggccagggtt tcggcaagcc cggcgaggac ggctatgtcc
      721 ggatcatcgg cgtcgccctg gacgtcaccg aggaacgcct ggcccaggcc cgcgcccagg
      781 ccgccgagaa ccgtctgcgc gacgccatcg agagcgtgtc ggaagccttc gtcctgtggg
      841 accgccaggg gcggctgctg atgtgcaacc gcaactatcg tagcgtcttc aaccttgagc
      901 ccaagctgct gaagccgggc gcgccgcgcg ccgaggtcaa ccgcttcgcg gccctggcca
      961 tcaagcagga ccatcccgcc cccgacggcg ccaagggcgt gcgcgaggcc gagctgatgg
     1021 atggtcgctg gattcagatc agcgagcgcc gcacggccga aggcggcctg gtcatgaccg
     1081 ctgccgacat caccgccatc aagaaccagg aagaagcccg ccgcc
//
LOCUS       DAOMEO010001215         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12844_length_1125_cov_2.859813, whole genome shotgun sequence.
ACCESSION   DAOMEO010001215 DAOMEO010000000
VERSION     DAOMEO010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12844_length_1125_cov_2.859813"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="PLH31_19840"
     CDS             <1..130
                     /locus_tag="PLH31_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91477.1"
                     /translation="EAALFARNITDEDNLKGAIDFNNLTAFVNEPRVIGLSLNVKM"
     gene            complement(185..490)
                     /locus_tag="PLH31_19845"
     CDS             complement(185..490)
                     /locus_tag="PLH31_19845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HQR91478.1"
                     /translation="MYGLIGQMMAAPGKRDELITILGEGTQAMPGCLSYIVAKDATNA
                     DAIWITEVWTDAEHHTASLKLPSVQAAITRARPMITGFGQRFETIPVGGVETPKTQA"
     gene            complement(603..956)
                     /locus_tag="PLH31_19850"
     CDS             complement(603..956)
                     /locus_tag="PLH31_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HQR91479.1"
                     /translation="MALRSWLLALTTVLLLGCESPGASAPSSPPPMTRAPVAEGSMCG
                     GIAGFQCAEGLSCQMQAEQCRVIADASGICRKRPTMCTREYRPVCGCDGKTYGNACTA
                     AAKGVSVAAPGECKP"
BASE COUNT          203 a          369 c          340 g          213 t
ORIGIN      
        1 ggaagcggcg ctgtttgccc gcaacatcac cgacgaggac aacctcaagg gcgcgatcga
       61 cttcaacaat ctgaccgcct tcgtcaacga gccgcgcgtc atcggcctgt cgctgaacgt
      121 gaagatgtag tccaccccga caccgggaaa cgacgaaggg ccgcggagca atctgcggcc
      181 cttttcacgc ctgggtcttg ggagtttcga cgccgccaac cgggatggtt tcaaagcgct
      241 ggccaaaccc ggtgatcatc ggtcgggccc gcgtgatggc cgcctgcacc gacgggagct
      301 tcagagatgc ggtgtggtgc tcagcgtccg tccagacctc tgtgatccag atcgcatcgg
      361 cgttggtcgc atccttggcc acgatatagc tgaggcatcc cggcatggcc tgcgtgccct
      421 cgcccagaat ggtgatcaac tcgtcccgtt tgccgggcgc ggccatcatc tgaccgatca
      481 atccatacat cgatgcttgt tccctacttg tggcggccag aggcgcgccg actgtcacga
      541 cagcgacccc ggccgccaga acgtttcgcc gatctatctc gaaatctctc aagcttgccc
      601 cctcatggct tgcactcacc gggggccgcg acgctgacgc ctttggcggc cgccgtgcag
      661 gcgttgccat aggtcttgcc gtcgcagcca cagaccgggc gatactctcg ggtgcacatc
      721 gtgggacgct tgcggcaaat gcccgacgcg tcggcgatca cccggcactg ctcggcctgc
      781 atctggcaag atagcccttc agcgcactgg aagccggcga tcccgccgca catactgccc
      841 tcggcaaccg gtgcgcgggt catcggcggc ggtgaagacg gtgcgctggc gcccgggctt
      901 tcgcagccga gcaaaagaac cgtggtcaag gccagtagcc aggaacgcaa agccatcggt
      961 catctccgta gatcgggtgt tctcgcccaa cgcatgatga cgcgcccagc gcccggcgac
     1021 agacaaattt cacggaacgg ataatacgag acgccggctc tcttgcttgg tgaagttttt
     1081 tcgtctgtcc tgcccgcctg ttcgggagtc ggggtttttc atgat
//
LOCUS       DAOMEO010001216         1125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12853_length_1125_cov_1.933645, whole genome shotgun sequence.
ACCESSION   DAOMEO010001216 DAOMEO010000000
VERSION     DAOMEO010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12853_length_1125_cov_1.933645"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLH31_19855"
     CDS             complement(<1..458)
                     /locus_tag="PLH31_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR91480.1"
                     /translation="MDKRDLAIRRIVIVGGGTAGWMTAAGLSAVLGDSAVSIELVESA
                     EIGTVGVGEATVPHIRNFNAKLGLDEADFMRKTQATYKLGIEFRDWGKKGESYIHPFG
                     AFGQAIGGVAFHQHWLRARARGQADDIQAYSLPIVAARLNRFAPPSSDQRS"
     gene            828..>1125
                     /locus_tag="PLH31_19860"
     CDS             828..>1125
                     /locus_tag="PLH31_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91481.1"
                     /translation="MDSGKRPSAQRGQNSFMSGLKCGASAVALGAAIFAVPAYAQAQV
                     KTDDAAVVDEVVVTSIRQSLKSSQALKQSSDIIGDSITAEDIGALPDRSVTEALQ"
BASE COUNT          220 a          356 c          348 g          201 t
ORIGIN      
        1 gagcgctgat cggaagaggg cggagcgaac cggttcagcc gcgcggccac gatgggcagg
       61 ctgtaggcct ggatgtcatc ggcttggccc cgcgccctgg cccgcagcca gtgctggtgg
      121 aaggccacgc cgccgatcgc ctgaccaaag gcgccaaagg ggtggatata gctctcgcct
      181 ttcttgcccc agtcgcgaaa ctcgatgccc agcttgtagg tcgcctgggt cttgcgcatg
      241 aagtcggcct cgtcgaggcc cagcttggcg ttgaaattcc ggatgtgtgg cacggtggcc
      301 tcaccaaccc caacggtgcc gatttccgca gactcaacca gctcgataga gacagccgag
      361 tcgcccagca ccgcgctcag gcccgcagcg gtcatccatc cagcagtgcc gccgcccacg
      421 atgacgatcc gtcgaatcgc gaggtcacgc ttgtccatcc caatgtcctt ccgccccgtc
      481 tgcgcgggtc tttcatcaaa ataacaccaa gacaaagtcg aaattgacaa cgctgtctta
      541 ggcgggcatc gttctgtgac cggacggcaa gttccggctt gggcagccaa atcatcgggg
      601 ggaaaccgga ggtcgagcag catttgggtg cgtgtgtcgt acaggcgacg aaccccggct
      661 gaccggccct ttcagtctct cccccatgca cagctacgtg agccgaccct tcgtggcaac
      721 gcttgacagc cggtgctctg tcatatgatc tgaccaataa tggtcacggt aacatgacca
      781 atggtagcgc taacttgcgc tagccaaaaa tcggggaggt gagacacatg gactctggga
      841 agaggccgtc cgcgcagcgc ggacaaaatt cattcatgtc gggtctgaaa tgcggcgctt
      901 cggccgtggc gttgggcgcg gcgatcttcg ctgtcccggc ctatgcccag gcgcaggtca
      961 agaccgacga cgcggcggtt gtcgatgagg tcgtggtcac cagcatccgc cagagcctga
     1021 agagctcgca ggcgctgaag cagtcgtccg acatcatcgg cgactcgatc actgccgaag
     1081 acatcggcgc cctgccggac cggtcggtca ccgaggccct gcagc
//
LOCUS       DAOMEO010001217         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12868_length_1124_cov_2.523854, whole genome shotgun sequence.
ACCESSION   DAOMEO010001217 DAOMEO010000000
VERSION     DAOMEO010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_1124_cov_2.523854"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="lpdA"
                     /locus_tag="PLH31_19865"
     CDS             complement(<1..1104)
                     /gene="lpdA"
                     /locus_tag="PLH31_19865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQR91482.1"
                     /translation="MAEYDYDVLVIGAGPGGYVAAIRAAQLGLKVACAEGRETLGGTC
                     LNVGCIPSKALLHGSEKFDEAVNGTFDTYGIKTGPVTLDLTAMQGQKLDSVKQLTGGI
                     EFLFKKNKVTWLKGYANFEDAHTVTVAGQNVSAKNVIIATGSSVTPLPGVTVDNDAGV
                     IVDSTGGLALDRVPNHMVVIGGGVIGLELGSVWRRLGAKVTVVEFLDQLLPGMDGDVR
                     KEAAKIFKKQGMDLKLGTKVTGVSVSGKTATLTVEPSKGGEASTIEADCVLVAIGRRP
                     NVDGLGLDKIGLEVNARGQIETDHDFATKVPGVWAIGDVIPGPMLAHKAEDEGIAVAE
                     NIAGLTGIVNHDLIPGVVYTFPEFAGVGLTEEAA"
BASE COUNT          188 a          385 c          329 g          222 t
ORIGIN      
        1 cgcggcttcc tcggtcaggc caacgcctgc aaactcgggg aaggtgtaaa ccacaccggg
       61 gatgaggtcg tgattcacga tgccggttag gcccgcgatg ttctcggcca cggcgatgcc
      121 ttcatcttcg gccttgtgcg ccagcatcgg gccggggatc acgtcgccaa tggcccagac
      181 gcccggaacc ttggtggcaa agtcatgatc ggtctcgatc tgcccgcgtg cgttcacttc
      241 cagcccgatc ttgtcgaggc caaggccatc gacattggga cgacgcccga tggcgaccag
      301 cacgcagtca gcctcgatcg tcgaagcctc gccgcccttc gaaggctcga cggtcagcgt
      361 cgcggtcttg ccgctcaccg aaacgccggt caccttggtg ccaagcttca ggtccatgcc
      421 ctgcttcttg aaaatcttgg ccgcttcctt gcgcacgtca ccgtccatgc cgggcagcaa
      481 ctggtcgagg aattccacga ccgtcacctt ggcacccaga cgacgccaga ccgagcccag
      541 ctcaagcccg atcacgccgc cgccgatcac caccatgtgg ttcggcacgc ggtccagcgc
      601 cagtccgccg gtgctgtcca cgatcacgcc tgcatcgttg tcgacggtca cgccgggcag
      661 cggggtcacg cttgaaccgg tggcgatgat cacgttcttg gcgctgacgt tctggcccgc
      721 cacggtcacc gtgtgcgcgt cttcgaagtt cgcgtaaccc ttcagccagg tcaccttgtt
      781 cttcttgaac aggaactcga tgccgccggt cagttgcttg actgaatcca gcttttgacc
      841 ctgcatcgcg gtcaggtcga gcgtcaccgg gccggttttg atgccgtagg tgtcgaacgt
      901 gccgttcacg gcttcgtcga acttttccga cccgtgcagc agcgccttgg atggaatgca
      961 gccgacgttg aggcacgtgc cgcccagcgt ctcgcgccct tcggcacagg cgactttcag
     1021 ccccagctgt gccgcccgga tggcagcgac ataaccgccg ggaccggcac cgatgacaag
     1081 aacgtcgtaa tcgtattcag ccattttccg gcctccgttg cgcg
//
LOCUS       DAOMEO010001218         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12879_length_1123_cov_2.827715, whole genome shotgun sequence.
ACCESSION   DAOMEO010001218 DAOMEO010000000
VERSION     DAOMEO010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12879_length_1123_cov_2.827715"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLH31_19870"
     CDS             complement(<1..597)
                     /locus_tag="PLH31_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR91483.1"
                     /translation="MAGHSKFKNIMHRKGRADAARSKLFSKLSREITVAAKAGLPDPN
                     MNPRLRLAVNNAKAESLPKDVIDRAIKKSQMGDAADYAEIRYEGVAAGGVGIIVEVLT
                     DNRNRAAANVRSYFTKLGGNMGATGSVTFNFDHVGQIGYPAKAASEDEMMEAAIEAGA
                     DDVESDMSDEGEGHTVYTAFESLNEVAAALEARFGPASN"
     gene            complement(736..1074)
                     /locus_tag="PLH31_19875"
     CDS             complement(736..1074)
                     /locus_tag="PLH31_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HQR91484.1"
                     /translation="MDAISNARRLRRSQTLAESKLWTIVRGRRLHGFKFRRQVPVDRY
                     FADFLCREASLIVELDGASHDEREMHDALRTEVLERCGFRVLRFDNERVLTDPGGVAD
                     AIGAALRLQA"
BASE COUNT          184 a          341 c          384 g          214 t
ORIGIN      
        1 gttggaggcc gggccgaacc gggcttccag ggcggcggcc acctcgttga ggctttcaaa
       61 ggcggtatag accgtgtggc cctcgccctc gtccgacatg tccgactcga cgtcgtcggc
      121 gccagcctcg atggcggctt ccatcatctc gtcttcgctg gcggccttgg ccggatagcc
      181 gatctggccc acatgatcga agttgaaggt caccgagccg gtcgcgccca tattgccgcc
      241 cagcttggtg aagtaggagc gcacattggc ggcggcgcgg tttcggttgt cggtcagcac
      301 ctcgacgatg atgccaacgc cgccggcggc cacgccttcg tagcggattt ccgcatagtc
      361 ggccgcgtcg cccatctgcg acttcttgat cgcacggtcg atgacgtcct tgggcaggct
      421 ttcggccttg gcgttgttga cggcgaggcg caggcgcggg ttcatgttgg ggtcgggcag
      481 gccggccttg gccgccacgg tgatttcccg cgacagcttg gagaacagct tggagcgcgc
      541 ggcgtcggcg cggcccttgc ggtgcatgat gttcttgaat ttcgagtggc cggccattcg
      601 cggggctctt ctctgatctg acgaaggtgg agtgggcgcg cttctagcct ttggagggtg
      661 gttttgtcac ccaaaaggaa aacctctccc agagggagag gaggggcccg ccgcgcagcg
      721 gtgggaggtg aggggttacg cctgcagccg gagcgccgcg ccgatagcgt cggcgacgcc
      781 gcccggatcg gtcagaacgc gctcattgtc gaaccgcagc acccgaaacc cgcagcgctc
      841 cagaacttcg gttcgcaggg cgtcatgcat ctcccgctcg tcatgcgagg cgccatccag
      901 ctccacgatc aggctggcct cgcgacacag gaagtcggcg aaatagcgat caaccggaac
      961 ttggcgtcgg aatttgaagc cgtgcaggcg gcgaccacgg acgatcgtcc aaagcttgga
     1021 ttcggcgagg gtttggctgc gtcggagacg gcgggcgttc gagatagcgt ccatgatccg
     1081 cgaacatgca atcaaccttt gcgtgcatca acggcagtaa tgc
//
LOCUS       DAOMEO010001219         1122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12906_length_1122_cov_2.190253, whole genome shotgun sequence.
ACCESSION   DAOMEO010001219 DAOMEO010000000
VERSION     DAOMEO010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12906_length_1122_cov_2.190253"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLH31_19880"
     CDS             <1..651
                     /locus_tag="PLH31_19880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQR91485.1"
                     /translation="MTRTMSLISRDACSIRSIMIFSTVGLLSFGLFQAGWMPGPTLAR
                     DLGPVWFWISLILMIIGHDAWFYWTHQLIHDRRLFRTFHRRHHRSNNPSPFTAYSFDL
                     GEAAINALFVPLWMMIVPTQWPAAGLFMLHQIVRNTLGHSGYELFPARKDGRPLLSWL
                     TTVTHHDLHHAQAGYNYGLYFTWWDRLMGTEHPDYLARFAAVTAGKRQATSSPSPA"
     gene            complement(626..>1122)
                     /locus_tag="PLH31_19885"
     CDS             complement(626..>1122)
                     /locus_tag="PLH31_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91486.1"
                     /translation="AKLTAKVAGSLENPTYDLDLNLIRKPDADRFGKRSLALGASWNE
                     REAKLRSSLSRAFGFGLLPRANYVSVSMNMDASRAIAVPGQIPVARANVRSALTLDGK
                     PVGSIALGGGSMGDEGVDLSLTRPFDIPAEFTVSLRAAALNGGEDWRLQDGRFMVKLV
                     KANW"
BASE COUNT          186 a          375 c          358 g          203 t
ORIGIN      
        1 atgacgcgga caatgtcgct gatatcgcgc gacgcctgct cgatccgctc catcatgatc
       61 ttctcgacgg tagggctctt gagctttggc ctgttccagg ccggctggat gccgggcccg
      121 accctcgcgc gggatcttgg gcccgtatgg ttctggatca gcctgatcct gatgatcatc
      181 ggccatgacg cctggtttta ctggacgcac cagctgatcc acgaccggcg gctgtttcgg
      241 acctttcacc ggcggcatca ccggtcgaac aatccctcgc ccttcaccgc ctacagcttc
      301 gatctgggcg aggcggcgat caatgcgctg ttcgtgccgc tgtggatgat gatcgtaccc
      361 acccagtggc cggcggcggg cctgttcatg ctgcaccaga tcgtccgcaa cacattgggc
      421 cacagcggct acgaactgtt cccggcgcgc aaggacggtc gacctctgct ctcctggctg
      481 accacggtga cgcatcacga cctgcaccac gctcaggccg gctacaatta cgggctctat
      541 ttcacctggt gggatcgcct gatgggcacc gagcacccgg actatctggc gaggttcgcc
      601 gccgtgacgg cgggaaagcg gcaggctacc agttcgcctt caccagcttg accatgaagc
      661 gaccgtcctg cagccgccag tcttcgccgc cattgagcgc ggcggcgcgc aggctgacgg
      721 tgaactcggc ggggatgtcg aagggccggg tcaggctgag atcgacaccc tcgtcgccca
      781 tcgaaccgcc ccccagggcg atggagccga ccggcttgcc gtccagggtc agggccgagc
      841 gcacattggc ccgcgccacc ggaatctgac ccggaacggc gatggcgcgg ctggcgtcca
      901 tgttcatcga gacgctgaca tagttggcgc ggggcagcag gccgaaaccg aacgcccggg
      961 acaacgacga gcgcagcttg gcctcgcgct cgttccacga cgcccccagg gccagcgatc
     1021 gcttgccaaa gcggtctgcg tccggtttgc ggatcaggtt gaggtcgaga tcgtaggtcg
     1081 ggttctcaag cgaaccggcc accttggccg tcagcttggc gg
//
LOCUS       DAOMEO010001220         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12912_length_1121_cov_2.388368, whole genome shotgun sequence.
ACCESSION   DAOMEO010001220 DAOMEO010000000
VERSION     DAOMEO010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12912_length_1121_cov_2.388368"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLH31_19890"
     CDS             <1..680
                     /locus_tag="PLH31_19890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQR91487.1"
                     /translation="SVVNGRSVYDVRKRMGQRLAIETGVEADVVVPVPDSGVPAAIGY
                     AQESGLPFDLGIIRNHYVGRTFIQPTQGVRELGVRMKHSPNRAVLEGKRVILIDDSIV
                     RGTTSLKIVRMVREAGAKEVHLRSASPPIKWPDFYGIDMPEREQLLAANKSLEEMARF
                     LEVDSLGFLSVDGLYQALEAGPRDPVTPQFTDHYFTGDYPTRLTDREVAEGRNEANDK
                     QLSLLSA"
     gene            690..>1121
                     /locus_tag="PLH31_19895"
     CDS             690..>1121
                     /locus_tag="PLH31_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HQR91488.1"
                     /translation="MSGLSKLLANLKIDGYIILLFGMVVLASILPVRGEIAGGLSLVV
                     KLAIALLFFLHGAKLSREAVVAGVTHWRLHLTILAFTYVLFPILGVMAAKTGVLSPTM
                     AAGMVFLACLPSTVQSSIAFTSIARGNVAAAVCAASASNLLG"
BASE COUNT          177 a          394 c          352 g          198 t
ORIGIN      
        1 acagcgtcgt gaatggccgc tcggtctatg acgtgcgcaa gcgcatgggc cagcgcctgg
       61 ccatcgagac cggcgtcgag gccgacgtcg tcgtgcccgt acccgacagc ggcgtgccgg
      121 ccgccatcgg ctatgcgcag gagagcggcc tgcccttcga cctgggcatc atccgcaacc
      181 actatgtggg ccgcaccttc atccagccga cgcaaggcgt ccgcgaactg ggcgtgcgca
      241 tgaagcacag ccccaaccgc gccgttctgg aaggcaagcg cgtcatcctg atcgacgact
      301 cgatcgtacg cggcaccacc tcgctgaaga tcgttcgcat ggtccgcgag gccggcgcca
      361 aggaggttca cctccgctcg gccagcccgc cgatcaagtg gccggacttc tacggcatcg
      421 acatgcccga gcgcgaacag ctgctggcgg ccaacaagtc gctggaagaa atggcgcggt
      481 tcctggaagt cgacagcctg ggcttcctgt cggtggacgg cctctatcag gcgctggaag
      541 ccggtccgcg tgatccggtc accccccagt tcaccgacca ctatttcacc ggcgactatc
      601 ccacccgcct gacggaccgc gaagtcgccg agggccgcaa cgaggccaac gacaagcaac
      661 tgtcgctgct cagcgcataa cccggcgtga tgagcggact ttccaagctt ctcgcgaacc
      721 tgaagatcga cggttacatc atcctgctgt ttggcatggt ggtgctggcc tcgatcctgc
      781 cggtgcgcgg cgagatcgcc ggcggcctga gccttgtcgt caagctggct atcgccctgc
      841 tgttctttct gcacggcgcc aagctgtcgc gcgaggccgt cgtcgcgggc gtgacgcact
      901 ggcggctgca cctgaccatc ctggccttca cctatgtcct gttcccgatc ctgggtgtga
      961 tggccgcgaa gaccggcgtg ctgtcgccga ccatggcggc ggggatggtg ttcctggcct
     1021 gcctgccctc gaccgtgcag tcgtccatcg ccttcacctc gatcgcccgg ggcaatgtcg
     1081 ccgccgccgt ctgcgcggct tcggcctcga acctgctcgg c
//
LOCUS       DAOMEO010001221         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12913_length_1121_cov_2.193246, whole genome shotgun sequence.
ACCESSION   DAOMEO010001221 DAOMEO010000000
VERSION     DAOMEO010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_1121_cov_2.193246"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>469
                     /locus_tag="PLH31_19900"
     CDS             149..>469
                     /locus_tag="PLH31_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR91489.1"
                     /translation="MSASADIAAKGRKTIFITGAASGIGLASAKRFAAAGWFVGLSDI
                     DVAGLKAALLAIGPANGATYRLDVRDREAWDNALGEFGRLTDGKLDALLNNAGVARFG
                     SLEDL"
     assembly_gap    470..479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <480..948
                     /locus_tag="PLH31_19905"
     CDS             <480..948
                     /locus_tag="PLH31_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR91490.1"
                     /translation="INIKGVIHGARAGLALLKAGGGRLINVASCAGLYGSPKLAVYSA
                     TKFAVRGLSEALDIEYAAHGVSVACVMPWFIETPILNAGASGSNENMADALKKGGLEV
                     YPVEDAAEVVFKSATGKELHYLVGKRAKQMRFATSHMPGAVRKQLRSRPLMAS"
     gene            complement(908..>1121)
                     /locus_tag="PLH31_19910"
     CDS             complement(908..>1121)
                     /locus_tag="PLH31_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91491.1"
                     /translation="GKGMASIGGVAYAANAVATPVAQPAQTGAGMGGPLGQATEPASA
                     PAKPGVEKTSADSVRTPSAAASATAS"
BASE COUNT          184 a          382 c          381 g          164 t
ORIGIN      
        1 gatcaagaag aacggccccg aggccgagac cgacgatcag acggactgat ctggacgtcc
       61 gcgcccgcgt ccttcacggg cgcgggcctt gacccttccc cctctccggc gtctgatcgc
      121 tgataataaa cgagggggag gatcgccaat gagcgccagc gccgacattg ccgccaaggg
      181 ccgcaagacc atcttcatca ccggggccgc cagcggcatc ggcctggcca gcgccaagcg
      241 cttcgccgcg gccggctggt tcgtggggtt gtccgacatc gacgtcgcgg gactgaaggc
      301 cgcgctgctg gccatcggcc cggccaatgg cgcgacctac cggctcgacg tccgcgaccg
      361 cgaggcctgg gacaacgccc tgggcgaatt tggccgactg acggacggca agctggacgc
      421 cctgctcaac aatgccggcg tggcccggtt cggatcgctg gaagacctcn nnnnnnnnnc
      481 atcaatatca agggcgtcat ccacggcgcg cgggccggcc ttgcgctgct gaaggccggc
      541 ggcgggcgat tgatcaacgt ggcctcctgc gcaggcctct atggctcgcc caagctggcg
      601 gtctattcgg ccaccaagtt cgccgtgcgc ggcctatctg aagccctcga catcgaatac
      661 gccgcccacg gggtgagcgt ggcctgcgtg atgccgtggt tcatcgagac gccaatcctc
      721 aacgccgggg cgtcaggcag caacgagaac atggccgacg ccctcaagaa gggcggactg
      781 gaggtctatc ccgtcgagga cgccgccgag gtggtgttca agtccgccac cggcaaggag
      841 ctgcactacc tggtgggcaa gcgggccaaa cagatgcgct tcgccaccag ccacatgccc
      901 ggcgcggtca ggaagcagtt gcgctcgcgg ccgctgatgg cgtcctgacg ctgtcggccg
      961 aggttttctc gaccccgggc ttggccggag ccgaagccgg ctcggtggcc tgacccagcg
     1021 gaccgcccat gccggcgcca gtctgagcgg gctgggcgac tggcgtggcg acggcgttgg
     1081 ccgcataggc gacgccgccg atggacgcca tgcccttgcc g
//
LOCUS       DAOMEO010001222         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12919_length_1121_cov_1.667917, whole genome shotgun sequence.
ACCESSION   DAOMEO010001222 DAOMEO010000000
VERSION     DAOMEO010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12919_length_1121_cov_1.667917"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1121
                     /locus_tag="PLH31_19915"
     CDS             <1..>1121
                     /locus_tag="PLH31_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR91492.1"
                     /translation="AAFAHWRGHHGAFGFARGGVTYTTAETHQIIADRLGESAVYGGR
                     ATGIGPRYCPSIEDKVVRFADKTSHQVFLEPEGLDDTTVYPNGISTSVSEETQLMFLR
                     TIPGLEQVEVKRYGYAIEYDYVDPRELFPTLETKRLPGLYLAGQINGTTGYEEAGAQG
                     LVAGLNAALAAQGREPAVFARDEAYIGVMIDDLVTRGVTEPYRMFTSRAEFRLTLRAD
                     NADQRLTDRGIVLGVVGPARAEAWTDKKAKLEAARAFARSVSLTPPEALKAGFKVKED
                     GERRNIFAMLAYPDVTLDRLAEVWPEVSTWNMAVREQIEIEAAYAGYLDRQRADAESF
                     RKEEDLRLPADLDYRAVGSLSNEVREKLARVKPLTLGQA"
BASE COUNT          192 a          379 c          366 g          184 t
ORIGIN      
        1 ctgcagcttt cgcgcattgg cgtggccatc atggcgcttt cgggtttgct cgcggcggcg
       61 tcacctacac gacggccgaa acccaccaga tcatcgccga ccgtctcggc gagtccgctg
      121 tctatggcgg ccgcgccacg gggatcggcc cgcgctactg cccgtcgatt gaggacaagg
      181 tcgtccgctt cgccgacaag accagccatc aggtgttcct ggaacccgag ggtctcgacg
      241 acaccacggt ctatccgaac ggcatctcga catctgtttc ggaagaaacg cagctgatgt
      301 tcctgcgcac gatccccggc ctggagcagg tcgaggtcaa gcgctacggc tatgcgatcg
      361 aatacgacta tgtcgatccg cgcgagctgt tcccgaccct ggagaccaag cgtctgccgg
      421 gcctgtatct ggccggccag atcaatggca ccacggggta tgaagaagcc ggcgcgcagg
      481 gcctggtcgc gggcctcaat gctgctttgg ccgcgcaggg cagggagccc gccgtgttcg
      541 cgcgggacga ggcctatatc ggcgtgatga tcgacgacct ggtcacccgt ggcgtcaccg
      601 agccctatcg catgttcacc agccgggccg agttccgcct gaccctgcgc gccgacaatg
      661 ctgaccagcg cctcaccgac cggggcatcg tcctgggcgt cgtcgggccg gcgcgcgccg
      721 aggcctggac tgacaagaag gccaagctgg aagcggcccg cgccttcgcc cgctcggtca
      781 gcctgacccc gccggaagcg ctgaaggccg gcttcaaggt caaggaagac ggcgagcgcc
      841 ggaacatctt cgccatgctg gcctatcccg acgtgaccct ggatcgtctg gctgaggtgt
      901 ggcccgaggt ttccacgtgg aacatggcgg tgcgcgagca gatcgagatt gaagccgcct
      961 atgccggcta tctcgaccgc cagcgcgccg acgccgaatc cttccgcaag gaagaggacc
     1021 tgcgcctgcc ggccgatctc gactatcgag ccgtgggcag cctttccaac gaggtccgcg
     1081 agaagctggc gcgggtgaag cctctcaccc tgggccaggc g
//
LOCUS       DAOMEO010001223         1120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12935_length_1120_cov_1.920188, whole genome shotgun sequence.
ACCESSION   DAOMEO010001223 DAOMEO010000000
VERSION     DAOMEO010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_1120_cov_1.920188"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLH31_19920"
     CDS             complement(<1..847)
                     /locus_tag="PLH31_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HQR91493.1"
                     /translation="MPQTLDLFPIGNCAVSALIDRAGRFVWSCAPRVDSDPVFSALLS
                     DVEPQGSGATGVWDVDVEDSVSTTQAYLRNTPILRTEITDADGARIEIIDFAPRYQQF
                     GRIFRPTAFIRLIRPVAGTARITIRLRPTAQWGARLAERTSGSNHIRYLCSDLTMRLT
                     TDCPVSHILEERTFRLEQPIALFLGPDEGFDADIGSTCERMLRETTAYWMTWVRGLAI
                     PLEWQSAVIRAAITLKLCVHEETGAIVAALTTSIPEHSDSGRNWDYRYCWMRDAYYVV
                     QALNRL"
BASE COUNT          202 a          328 c          398 g          192 t
ORIGIN      
        1 ccagccggtt cagggcctgg accacgtagt aggcgtcgcg catccagcag tagcggtagt
       61 cccagttgcg accgctgtcg gaatgctcgg gaattgaggt ggtcagcgcc gcgacgatgg
      121 cgcccgtctc ttcgtggaca cagagcttga gggtgatcgc cgcacggatg acagctgact
      181 gccactccag cggaatggcc agaccccgga cccaggtcat ccagtaggcg gtggtttcgc
      241 gcagcatgcg ctcgcaggtc gacccgatgt cggcgtcaaa accttcgtcg ggaccaagga
      301 acagggcgat gggctgctcc aggcggaagg tgcgttcttc gaggatgtgc gacaccgggc
      361 agtccgtggt cagccgcatc gtgaggtccg agcacaggta gcggatgtgg ttggaccccg
      421 aggtccgctc ggccagccgc gccccccact gggcggtcgg gcgcaggcgt atcgtgatcc
      481 gcgccgtgcc agccacaggc cggatgaggc ggataaaggc cgtgggtcga aagatccggc
      541 caaactgctg gtagcgtggc gcgaaatcga tgatctcgat cctggcgccg tcggcatcgg
      601 tgatttcggt gcgcaggatc ggcgtgttgc gaagataggc ctgggttgtt gacaccgagt
      661 cctcgacatc gacatcccag acgccggtcg ccccgctgcc ctgcggctct acatcactga
      721 gcagcgcgct gaacacggga tcagagtcga cgcgaggggc gcaagaccag acgaagcgcc
      781 cagcacggtc gatgagcgcg ctcacggcgc agttgccgat ggggaagagg tcgagagttt
      841 ggggcacgcg gtctccggat tgtggcgaga cctaaggtcg gcctgacgac acaagggaaa
      901 ccgcttgtgt ggcttcccgg ttcctgctgg cagaaaagaa cgctgtggac cagtgagggt
      961 tcgcgtttca cgatactcgc gttgacggac gctgcttcca ccagccattt gaggcggcgc
     1021 ggacacatcg cgcacggtgt gcggcgagat tcattggcgg tgagcagttt gtcgagacta
     1081 acctgcgcgc tggccgccgg agcgctatgg cttgcggcgg
//
LOCUS       DAOMEO010001224         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12948_length_1119_cov_2.479323, whole genome shotgun sequence.
ACCESSION   DAOMEO010001224 DAOMEO010000000
VERSION     DAOMEO010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12948_length_1119_cov_2.479323"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..1119)
                     /locus_tag="PLH31_19925"
                     /pseudo
     CDS             complement(352..1119)
                     /locus_tag="PLH31_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
BASE COUNT          179 a          344 c          372 g          224 t
ORIGIN      
        1 atgggcgacg cgaccatcac cctttagcca acgcccaaag tcgcactgac agtccgatgc
       61 aatccgcgag gcgtcgagcg tcttttgcga gctgatggca atacggagat tgaccttcca
      121 gtcggaatgc gcggccaggg ctgtatctaa atccatgttc ttgggtctcc ttcatcggcc
      181 agtaacggcg agcccgatga aggaagactt agagcccatg cttaacgccg ccctggaaac
      241 ccgatccggg gttgaccttg caaagtcttg gcgtgggcgc ccgaaagggt ctggagacta
      301 tttcttctcg accacgggct tgcccatcgc cgctgccttg gccgtgatat ctgcgttctt
      361 gaagctggcc tcgctgaacc atacgcggtt ggcgatatcc tgggcctggt cgcgggtctg
      421 ggcgacctcg agatcaggcg cgatgccggt cttctggatc gagcgacccg agggggtgaa
      481 gtacttggcc gtggtcagct tcagggcgcc gtcggcgccg ccccgcaggg ggatcacggt
      541 ctggaccgaa cccttgccaa agctggtcag cccgaccagt tcggcgcgct tgcggtcctg
      601 cagggcgccg gcgacgattt cagcggccga ggccgagccc tgattgatca gcaccaccac
      661 gggcaggccg ttcagcaggt cgccggggcg ggcgttgtag cgctggatgt tgcgcgggtc
      721 gcgaccgcgc tggctgacca cctcgccgcc gtccaggaac acgtcggcca cgcccaccgc
      781 ctggtcgagc agaccgccgg ggttgttgcg catgtccagc acaaggccct tcatcttcgg
      841 gttcttggcc ttcaggtcgt tgatcgacga gaccagggcg tcggtggcct tttcgttgaa
      901 gccgggcagg cgcacatagc catagtcgcc ttccatgcgg gcgatggcgg ccttgggctt
      961 gatgacttcg cggatcagct tgacgtcgaa ggggtcggtc ttttcgcggg cgatggtcag
     1021 ggtgacaggt tcgccggcca cgccgcgcat ctgtttgacg gcctcattga cggtcaggcc
     1081 cagcacgctc tggctgttga cggcggtgat atagtcgcc
//
LOCUS       DAOMEO010001225         1119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12952_length_1119_cov_1.936090, whole genome shotgun sequence.
ACCESSION   DAOMEO010001225 DAOMEO010000000
VERSION     DAOMEO010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12952_length_1119_cov_1.936090"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..335
                     /locus_tag="PLH31_19930"
     CDS             9..335
                     /locus_tag="PLH31_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HQR91494.1"
                     /translation="MEAVDPTVFRLAIFVLAIFVGYYVVWSVTPALHTPLMAVTNAIS
                     SVIIVGALLAAAAHGGAGEATTSSVWISKGAGALAAAFAAVNIFGGFLVTNRMLAMYK
                     KKDKAK"
     gene            332..628
                     /locus_tag="PLH31_19935"
     CDS             332..628
                     /locus_tag="PLH31_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91495.1"
                     /translation="MSGFAALTLAALIVLIIAAGVSMVLPRRKSAGNGAAFAPAFVLI
                     AIGLSANAVGLQAGTSGAADILRWLSPFAVGMGAALLLSNLRADKAPPSGGTSE"
     gene            625..>1119
                     /locus_tag="PLH31_19940"
     CDS             625..>1119
                     /locus_tag="PLH31_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQR91496.1"
                     /translation="MNANIAAILYIVSGVLFILALRGLSSPVTSQAGNRNGMIGMAIA
                     VGTTLATLWSQGALDIVTLGLILGGVAVGGGVGAVIARKVPMTSMPQLVAAFHSLVGM
                     AACLVAVAAIYTPAAYGIVGEDGHIHVNSLIELSLGLAIGAITFTGSVVAFAKLNGNM
                     GGAPI"
BASE COUNT          151 a          411 c          359 g          198 t
ORIGIN      
        1 aggctcccat ggaagccgtc gaccccaccg tgttccgcct ggcgatcttc gtgctcgcca
       61 tctttgtggg ctattacgtc gtgtggagcg tgacgcccgc cctgcacacg ccgctgatgg
      121 ccgtgaccaa cgccatttcc tccgtgatca tcgtgggggc cctgctggcc gccgccgccc
      181 atggcggcgc gggcgaggcc accaccagct cggtctggat ctccaagggg gccggggccc
      241 tggccgcagc cttcgcggcg gtcaacatct tcggcggctt cctggtcacc aaccggatgc
      301 tggccatgta caagaagaag gacaaggcca agtgagcggc ttcgccgccc tgaccctggc
      361 ggccctgatc gtgctgatca tcgccgccgg cgtgtcgatg gtgctgcctc ggcggaaatc
      421 cgccggcaat ggcgccgcct ttgcgcccgc cttcgtcctg atcgccatcg gcctcagcgc
      481 caacgccgtc ggcctgcagg ccggaaccag cggcgcggcc gacatccttc gctggctctc
      541 gccctttgcc gtcggcatgg gcgcggcgct gctgctctct aatctgcgcg ccgacaaggc
      601 gccgccgtcc ggggggacct ccgaatgaac gccaatatcg ccgccatcct ctacatcgtc
      661 tcgggggtgc tgttcatcct ggccctgcgc ggcctgtcca gcccggtgac cagccaggcg
      721 ggcaatcgca acggcatgat tggcatggcc attgccgtcg gcaccactct ggccaccctg
      781 tggagccagg gcgctctgga catcgtaacc ctgggtctga tcctcggcgg cgtcgccgtc
      841 ggtggcggcg tgggcgcggt gatcgcccgc aaggtgccga tgacctcgat gccgcaactg
      901 gtcgccgcct tccacagcct ggtcggcatg gccgcgtgcc tcgtggccgt ggccgccatc
      961 tacaccccgg ccgcctatgg catcgtcggc gaggatggcc acatccacgt caacagcctg
     1021 atcgagctat cgctgggcct ggccatcggc gccatcacct tcaccggctc ggtcgtggcc
     1081 ttcgccaagc tgaacggcaa catgggcggc gcgccgatc
//
LOCUS       DAOMEO010001226         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_12991_length_1117_cov_1.984934, whole genome shotgun sequence.
ACCESSION   DAOMEO010001226 DAOMEO010000000
VERSION     DAOMEO010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12991_length_1117_cov_1.984934"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="PLH31_19945"
     CDS             complement(<1..>1117)
                     /locus_tag="PLH31_19945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR91497.1"
                     /translation="PPMCLVIAPTRELAIQVNRELTWLYADAGAVVVNCVGGMDARRE
                     QRALNWGAHVVVGTPGRLRDHIERGHLDLSELKVAVLDEADEMLDMGFREDLEFILDA
                     APAERRTLLFSATLAREIVQLAKSYQNDALRIDTVGRNEPHRDIEYRAVRVAPNEIEH
                     AIVNLLRYFESPGALVFCNTRESVRAMHSKLRERGFAVVGLSGELSQRERADALQALR
                     DGHARVCVATDVAARGLDLPDLGLVIHAELPVNKATLLHRSGRTGRAGKKGVSALVVP
                     YTRRRKAEQLLMAAGVEGHWGGAPTADEIRVKDTERLLDDPIFDEVAVEEDTALAEAM
                     LAKRTPLEIASALIRTRRAKLPAPEEIYDDPRHGADPG"
BASE COUNT          157 a          358 c          422 g          180 t
ORIGIN      
        1 ggcccggatc ggcgccgtga cgcgggtcgt catagatttc ttccggagcc ggcagcttgg
       61 cgcggcgcgt gcggatcagg gcggaggcga tctcaagcgg ggtgcgcttg gccagcatgg
      121 cctcggccag agcggtgtct tcctcgaccg cgacctcgtc aaagatcgga tcgtccagca
      181 ggcgctcggt atccttgacg cggatctcgt cagcggtcgg ggcgccgccc cagtggcctt
      241 cgacgccggc ggccatcagc agctgctcgg ccttgcgacg acgggtgtag ggcacgacca
      301 gggccgaaac gcccttctta ccggcccggc cggtgcggcc cgaacggtga aggagggtgg
      361 ccttgttgac cggcagttcg gcgtggatga cgaggcccag gtccggcagg tccagaccgc
      421 gcgcggcgac gtcggtggcg acgcagacgc gggcatggcc gtcgcgcagg gcttgcaggg
      481 cgtcggcgcg ttcacgttgc gacagctcgc ccgacaggcc gaccacggcg aagccgcgct
      541 cgcgcagctt gctgtgcatg gcgcggacgc tttcgcgggt gttgcagaac accagggcgc
      601 cggggctttc gaaatagcgc agcaggttga cgatcgcgtg ctcgatctcg ttcggcgcga
      661 cccgcacggc gcgatattcg atgtcgcggt gcggctcgtt gcggccgacg gtgtcgatgc
      721 gtagggcgtc gttctgatag ctcttggcca gctgcacgat ctcgcgcgcc agggtggccg
      781 agaacagcag ggtgcggcgt tcggccgggg cggcgtcgag gatgaattcc aggtcttcgc
      841 ggaagcccat gtcgagcatc tcgtcggcct cgtcgaggac ggcgaccttc agctccgaca
      901 ggtccaggtg gccgcgctcg atgtggtcgc gcagacggcc gggggtgccg accaccacat
      961 gggcgcccca gtttagcgcg cgttgctcgc ggcgagcgtc catgccgccc acgcagttga
     1021 cgaccacggc gccggcgtcg gcgtacagcc acgtcagctc gcgattgacc tggatggcca
     1081 gttcgcgggt cggggcgatg accaggcaca tgggcgg
//
LOCUS       DAOMEO010001227         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13011_length_1116_cov_2.129123, whole genome shotgun sequence.
ACCESSION   DAOMEO010001227 DAOMEO010000000
VERSION     DAOMEO010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13011_length_1116_cov_2.129123"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLH31_19950"
     CDS             <1..949
                     /locus_tag="PLH31_19950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR91498.1"
                     /translation="TRMLDETITAYGQVEFNPSAVRTLTATFEAQVAKIHVTVGEAVQ
                     AGQPIVTLMASPTSRLDLDRLAREASVTSAEHARLQRLRADGLAADAEVTAAAAAAAT
                     AREASANLRRLTGGGQITLRAPVSGVIDSLPASPGALVSAGGAVAGLGSTSQLNARLG
                     IEVEDAQRIRPGAAIRLTGLHEDGAAVAARVASVDRRVEPSTRLAAVIVSLPPGSPVL
                     PGEALRGQIVLSTLPNATVAPRVAVLYDGDRPYVFVVVRNVASRRPVTVGLEQADVVQ
                     LTSGVRPGERVVVDGGPALSDGMMTREGPPPATATAAKQ"
BASE COUNT          158 a          403 c          376 g          179 t
ORIGIN      
        1 aacccggatg ctggacgaga ccatcacggc ttacggccag gtggagttca acccctccgc
       61 cgtacggacc ctcacggcga cctttgaggc ccaggtggcc aagatccacg tcacggttgg
      121 cgaagccgtg caggccggtc agccgatcgt gaccctcatg gccagcccga cgagcaggct
      181 cgatctggat cggctcgccc gtgaggccag cgtgacgtcc gccgaacatg cgcgtcttca
      241 gcgacttcgc gccgacgggc tcgccgcgga cgcggaagtc accgccgccg ctgctgctgc
      301 ggccacggcc agggaggcca gcgccaacct gcgccggctg accggtggag gccaaatcac
      361 gcttcgcgcg cccgtcagcg gcgtcatcga cagccttccc gcgagccccg gcgcgctggt
      421 cagcgcgggc ggggcggtgg ccggtctcgg ctcgaccagt cagttgaacg cgcgcctcgg
      481 gatcgaggtc gaggacgcgc agcgcatccg tcccggtgcc gcgatccgtc tcaccggcct
      541 gcacgaagac ggagccgctg tcgcggcccg cgtcgcttcg gtggaccgtc gggttgaacc
      601 ctctacccgc ctggccgccg tgatcgtctc cctgccgccc ggcagcccgg ttctgcctgg
      661 cgaggcgctc agggggcaga tcgtcctcag tacgctgccg aacgccaccg tcgcgccacg
      721 agtggctgtc ctctatgacg gagatcgacc ctatgtattc gtcgtggtcc ggaacgtcgc
      781 ctcgcgtcgg ccggttacgg tcggcctgga acaggccgat gtggttcaac tgaccagcgg
      841 cgtccgtccg ggtgaacggg tggtcgtcga cggcggacct gccctgtctg acggcatgat
      901 gacccgagag ggcccgcccc ccgcgacagc aacggcagcc aagcaatgag ccgctggctg
      961 agcggccagg tccgcttcat cattctggcc tttgcgttgt tggcgctgtc agggtccgcc
     1021 gctgccctgc gactgcccgt gagcctgttc ccgcacatcg acttcccacg cgtcgtggtc
     1081 acgctggacg ccggcgaccg tcctgtacag gacggt
//
LOCUS       DAOMEO010001228         1116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13025_length_1116_cov_1.612630, whole genome shotgun sequence.
ACCESSION   DAOMEO010001228 DAOMEO010000000
VERSION     DAOMEO010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13025_length_1116_cov_1.612630"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLH31_19955"
     CDS             complement(<1..234)
                     /locus_tag="PLH31_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HQR91499.1"
                     /translation="MTASPFDDIRALAYSPPAANAAPALGQSDRLADISSWLTAWTGK
                     TPPAVNRPVVALYAGARQGVGPRGYARDRLEAVA"
     gene            complement(283..444)
                     /locus_tag="PLH31_19960"
     CDS             complement(283..444)
                     /locus_tag="PLH31_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91500.1"
                     /translation="MIKTQRQGRADYIVEKASGAASAASGQARRADDAAKADLIAERD
                     GACQALLAR"
     gene            522..707
                     /locus_tag="PLH31_19965"
     CDS             522..707
                     /locus_tag="PLH31_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQR91501.1"
                     /translation="MATPCVKVCAVDGASGYCLGCRRTLAEIAGWGRLSDQDRADIMA
                     ALPDRPDPVASLMASQG"
     gene            complement(711..1040)
                     /locus_tag="PLH31_19970"
     CDS             complement(711..1040)
                     /locus_tag="PLH31_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91502.1"
                     /translation="MKTIIIAAVIASALTGVAQAAPKVSDNQFLQAARCRGLASSEGL
                     GRLDTAAIDQLLRTEGQTRTLAVKASANNKINAAQAQGDKADGAKKEKLLAERQEACA
                     TWMQGGQ"
BASE COUNT          160 a          381 c          389 g          186 t
ORIGIN      
        1 agcgacagcc tccagacggt cgcgcgcata gccgcgcgga cccacgccct gacgcgcccc
       61 ggcatacagc gccaccaccg gccggttcac ggcgggcggc gtcttgccgg tccaggcggt
      121 gagccaggac gagatgtccg ccagtcggtc ggactgaccc aaggcgggcg cggcgttggc
      181 ggccggaggg ctgtaggcca gggcgcggat atcgtcgaac ggtgaagcgg tcatagggcg
      241 ggctttggcg cccgcgtccg gtcttgtaaa gtcggttggc gcctaacggg ccagcaaggc
      301 ctggcaggcg ccatcgcgct cggcgatcag gtcagccttg gcggcgtcgt cagcccgccg
      361 cgcctggcca ctcgcggcgg atgcggcgcc cgaagccttc tcgacaatat agtcggcgcg
      421 cccctggcgt tgcgtcttga tcaaggcgct gaacgcggcg gcgtccccgg cgccgaggct
      481 ggaggccttg gccagggcct ggcaacgcgc cgccccgccc catcgccacg ccgtgcgtga
      541 aggtctgcgc cgtggacggc gcaagcggtt actgcctggg ctgtcgtcgc acgctggcgg
      601 aaatcgccgg ctggggccgc ctgagcgatc aggatagggc cgacatcatg gccgctctgc
      661 ccgatcgccc cgaccccgtg gccagcctga tggcgagcca gggttagagc ttactgaccg
      721 ccctgcatcc aggtggcgca ggcctcctgc cgctcagcca gcagcttctc cttcttggcg
      781 ccgtccgctt tgtcgccctg cgcctgagcg gcgttgatct tgttattggc cgaggccttg
      841 acggccaggg tgcgggtctg cccctcggtc cgcaggagct ggtcgatcgc ggcggtgtcc
      901 agccggccca gcccttccga tgacgcgagg ccgcgacagc gcgccgcctg caggaactgg
      961 ttgtcggata ccttcggtgc ggcctgagcg acgcccgtca gcgccgaagc aatgacggcg
     1021 gcgatgatga tcgttttcat tgtgcgttcc ctcgataagc aactctttgc ccggcgcaga
     1081 gatacttcca atgcaaacat agctgaagtt aattcg
//
LOCUS       DAOMEO010001229         1115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13045_length_1115_cov_1.926415, whole genome shotgun sequence.
ACCESSION   DAOMEO010001229 DAOMEO010000000
VERSION     DAOMEO010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13045_length_1115_cov_1.926415"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLH31_19975"
     CDS             <1..330
                     /locus_tag="PLH31_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQR91503.1"
                     /translation="RMKAAAEGEDDALSADIAFHVSILNATKNPFYRELHELVNTALR
                     ISIRFTNRIKGRTASIPSHEDVAEAIIARDADRAATAMRLIIVDVLELIRAASPSTES
                     EAARREA"
     gene            468..>827
                     /locus_tag="PLH31_19980"
     CDS             468..>827
                     /locus_tag="PLH31_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR91504.1"
                     /translation="MPYRRTGRSGLDLPAISLGLWQNFGGADVFETGRAILRRAFDLG
                     VTHFDLANNYGPPYGSAEENFGRVMATDFKAHRDELVISTKAGWDMWPGPYGGIGGSR
                     KYLIASCDQSLKRMGLDY"
     assembly_gap    828..837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <838..>1115
                     /locus_tag="PLH31_19985"
     CDS             <838..>1115
                     /locus_tag="PLH31_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR91505.1"
                     /translation="IFYSHRVDPTTPLEETMGALAHLHRTGKALYVGISSYSPELTRK
                     AHAILKAEGVPLLIHQPSYSMLNRWIEDALLDTLEDLGVGCIAFSPLA"
BASE COUNT          190 a          413 c          328 g          174 t
ORIGIN      
        1 cggatgaagg ccgccgccga gggcgaggac gacgccctgt cggccgacat cgccttccac
       61 gtctcgatcc tgaacgcgac caagaacccg ttctatcgcg agctgcacga gctggtgaac
      121 accgcgctgc gtatctcgat ccgctttacc aaccgcatca agggccgcac cgcctcgatc
      181 ccgtcgcacg aggacgtggc cgaggcgatc atcgcccgcg acgccgacag ggccgccacc
      241 gccatgcggc tgatcatcgt cgacgtgctg gaactgatcc gcgccgcctc gccttccacc
      301 gagtctgaag ccgcgcgccg cgaggcctag ataaccggac ttgccgcgcg cctgtcgcgc
      361 gctagattga caacgttgtc agaaatacga cgggtcgccg caccgcacgg cccgcaggag
      421 ggacgctcgc cgtgacctac accgccgcca ccaaccgcta tgacgccatg ccctatcgcc
      481 ggaccggccg cagcgggctg gaccttccgg ccatctcgct gggcctgtgg cagaacttcg
      541 gcggcgccga tgtgttcgag accggccgcg ccatcctgcg ccgcgccttc gacctgggcg
      601 tcacgcactt cgacctcgcc aacaactacg gcccgcccta cggctcggcg gaagagaact
      661 tcgggcgcgt gatggcgacc gatttcaagg cccatcgcga cgagctggtc atctccacca
      721 aggccggctg ggacatgtgg cccggcccct atggcgggat cggcggctcg cggaaatatc
      781 tgatcgccag ttgcgaccag agcctcaagc gcatggggct ggactatnnn nnnnnnncat
      841 cttctattcg caccgtgtcg atcccaccac accgctggaa gagaccatgg gcgccctcgc
      901 ccacctgcac cgcacgggca aggccctgta cgtcggcatc tcctcctatt cgcccgaact
      961 gacccgcaag gctcacgcca tcctgaaggc cgagggcgtg ccgctgctga tccaccagcc
     1021 gtcctattcg atgctcaatc gctggatcga ggacgccctg ctggacacgc tggaagacct
     1081 gggcgtcggc tgcatcgcct tctcgcccct ggccc
//
LOCUS       DAOMEO010001230         1114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13054_length_1114_cov_3.168083, whole genome shotgun sequence.
ACCESSION   DAOMEO010001230 DAOMEO010000000
VERSION     DAOMEO010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13054_length_1114_cov_3.168083"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLH31_19990"
     CDS             <1..666
                     /locus_tag="PLH31_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR91506.1"
                     /translation="HPDFVDGRIDTGFIEARLDELVERNFSDEPTMAAISWRLETFME
                     ADVKRDPWESAPSRLLGFRMNGGRAPMDLPMAVNGNEVPLRVALVGGPGEDWSWDIKH
                     RDGRVFGDVGRLPTSHGDSPIYVFEGGDVQEFEFGYQGGGAHSAVSDGAILSPMPGKI
                     VSVAVEAGQSVIKGQTLLTLEAMKMEHALAAPFDGVVAELSAVAGGQVSEGVVLAKLE
                     PAA"
     gene            677..1075
                     /locus_tag="PLH31_19995"
     CDS             677..1075
                     /locus_tag="PLH31_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HQR91507.1"
                     /translation="MALHIIKLVVGCDTIEDLLAWHGSGEPWIMHTRMTPKRIDEVLD
                     GGSLYRVFKGQVLCRQKILAIDTVGEGAAKRCEVTIDPPIIRVVPQPRRAFQGWRYLP
                     PEDAPADLTESEAADMPLELARQLRELGAW"
BASE COUNT          181 a          341 c          400 g          192 t
ORIGIN      
        1 caccccgact tcgtcgacgg ccggatcgac accggcttca tcgaggcgcg tctggatgag
       61 ctggtcgagc gcaacttctc ggacgagccg accatggccg cgatcagctg gcggctggaa
      121 acgttcatgg aagcggacgt gaaacgcgat ccgtgggaga gcgcaccctc gcgtcttctg
      181 ggtttccgga tgaatggcgg ccgcgcgcct atggacctac cgatggccgt gaacggcaat
      241 gaggtcccgt tgcgggtcgc cctggtcggc ggtcccggcg aagactggtc gtgggacatc
      301 aagcatcgag acgggcgggt ctttggagac gtcggtcgct tgccgacgtc gcatggcgat
      361 agcccgatct atgtcttcga gggcggcgac gttcaggagt tcgagttcgg ttatcagggg
      421 ggcggagccc attcggcggt gtctgacggc gcgatcctgt cgcccatgcc gggcaagatc
      481 gtctcggtgg ccgtggaagc cggccagagc gtcatcaagg gccagaccct gctgaccctg
      541 gaagccatga agatggagca cgccctggcg gcgccgttcg acggtgtcgt cgccgaactc
      601 tcggcggtgg ccggcgggca ggtcagcgag ggcgtggtgc tggccaagct ggaaccggcg
      661 gcttagtcac ccgtccatgg ctctgcacat tatcaagctg gttgtcggct gcgacacgat
      721 cgaagacctg ctcgcctggc acggatctgg cgagccttgg atcatgcaca cccgtatgac
      781 gcccaagcgc atcgacgagg tgctggacgg cgggtcgctg tatcgcgtgt tcaaggggca
      841 ggtgctgtgt cgccagaaga tcctggccat cgacacggtg ggcgagggcg cggccaagcg
      901 ctgcgaggtg acgatcgatc cgccgatcat ccgcgtggtt ccccagcccc ggcgcgcctt
      961 tcagggctgg cgctatctgc cgccggagga cgcaccggcc gatctgaccg agagcgaggc
     1021 ggcagatatg ccgctggagc tggcgcgcca gctgcgcgaa ctgggcgcct ggtagacgcc
     1081 tcgcctcagc cgacggccat gttgtcgatc agac
//
LOCUS       DAOMEO010001231         1113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13078_length_1113_cov_1.913989, whole genome shotgun sequence.
ACCESSION   DAOMEO010001231 DAOMEO010000000
VERSION     DAOMEO010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_1113_cov_1.913989"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLH31_20000"
     CDS             <1..227
                     /locus_tag="PLH31_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91508.1"
                     /translation="RATPVKAPACSTKGKTGKALKAAKARCAALAKKGGSKGKAVAKS
                     KKGSKAKATPARKGKATAKKAPVRKKPNLR"
     gene            complement(224..628)
                     /locus_tag="PLH31_20005"
     CDS             complement(224..628)
                     /locus_tag="PLH31_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQR91509.1"
                     /translation="MAEFGLDPAFVATSAALGDLPLCAARLQLDARYPWIVLIPRQPG
                     LREIEDLSPADRVALMDEIVMAGAAVRALGFQVDKLNVGALGNVTPQLHVHVIGRRLG
                     DPAWPGPVWGHSAAEPAALEAAAAAARAALGL"
     gene            complement(621..>1113)
                     /locus_tag="PLH31_20010"
     CDS             complement(621..>1113)
                     /locus_tag="PLH31_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR91510.1"
                     /translation="ALGGAPHAFEDYEIGERIDHVDGMAIEEAEHAMATRLWQNTAKV
                     HFNQFERARDPSGRRLVYGGVVISTAKALSFNGLQNAGLILAINGGRHVSPYFAGATV
                     FAWSEVLDKADLGDGVGALRLRLVATKDRPCDDFPGKDEAGVYNEHVILDFDYWAAVP
                     KRG"
BASE COUNT          193 a          404 c          376 g          140 t
ORIGIN      
        1 cccgcgcgac tccggtcaag gctcccgcct gctcgaccaa gggcaagacc ggcaaggcgc
       61 tgaaggccgc caaggcccgc tgcgccgccc tggccaagaa gggcggctcg aaaggcaagg
      121 ccgtcgccaa gagcaagaag ggctccaagg ccaaggcgac cccggcccgc aagggcaagg
      181 cgacggccaa gaaagccccc gtccggaaga agccgaacct gcgctagagg cccagcgccg
      241 ccctggccgc tgcggccgcc gcttcgaggg cggcgggctc ggcggcgctg tggccccaga
      301 ccggccccgg ccaggccgga tcaccgagcc ggcggccgat gacatgcaca tgcagttgcg
      361 gggtgacatt gcccagcgcg ccgacattga gcttgtcgac ctgaaagccc agcgcccgca
      421 ccgcggctcc ggccatgacg atctcgtcca tcagggcgac ccgatccgcc ggcgacaggt
      481 cctcgatctc gcgaagacct ggctggcgcg gaatcagcac gatccacgga tagcgggcgt
      541 ccagttgcag tcgggccgcg cacagcggca ggtcgcccag cgccgccgag gtggcgacga
      601 acgccgggtc cagcccgaac tcagccacgt ttcggaaccg cggcccaata gtcgaagtcg
      661 aggatgacgt gctcgttata gacgcccgcc tcgtccttgc ccgggaagtc gtcgcagggc
      721 cgatccttcg tcgcgaccag ccgcaggcgc agggcgccca cgccgtcgcc caggtcggcc
      781 ttgtccagca cctcgctcca ggcaaacacc gtggccccgg cgaaataggg gctgacatgg
      841 cggccgccat tgatcgccag gatcaggccg gcgttctgca ggccgttgaa cgacagggcc
      901 ttggccgtcg agatcaccac cccgccataa acgaggcggc gtccagacgg atccctggcg
      961 cgctcgaact ggttgaagtg gaccttggcg gtgttctgcc acagccgggt ggccatggcg
     1021 tgctcggcct cctcgatggc catgccgtcg acatggtcga tccgctcgcc gatctcgtaa
     1081 tcctcgaacg cgtgcggcgc gccgcccagg gcg
//
LOCUS       DAOMEO010001232         1111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13110_length_1111_cov_2.936553, whole genome shotgun sequence.
ACCESSION   DAOMEO010001232 DAOMEO010000000
VERSION     DAOMEO010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13110_length_1111_cov_2.936553"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLH31_20015"
     CDS             complement(<1..280)
                     /locus_tag="PLH31_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91511.1"
                     /translation="MNKILLSLTAGSALGAASVIPAVASAPAINQRQAMLERRIDQGL
                     RNGSLSRGEAYRLNTQLRETARLESRYRRGGLSGWERADLDRRFDRISA"
     gene            complement(413..>1111)
                     /locus_tag="PLH31_20020"
     CDS             complement(413..>1111)
                     /locus_tag="PLH31_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR91512.1"
                     /translation="EDGSGLRIDVADTGIGIAAEMQPRLFDKFVQADSSTTRQFGGSG
                     LGLAICRELATLMGGGIAVQSREGQGSTFTVVLAIPRGGEMTKAAAVVEDVQPKVSNR
                     PRVLAADDNPTNQKVIAAVLAPLGAEVELVADGAACVEAWKRGRHDIILMDIHMPVMD
                     GVEAAQTIRALERAEGRRRTPIVAVTANALAHQVEGYLAAGMDGHVAKPIEVTKLYAA
                     IEAAMTAARLADAA"
BASE COUNT          162 a          359 c          397 g          193 t
ORIGIN      
        1 gggcgctgat gcggtcgaag cgacggtcga ggtcggcgcg ttcccagccg ctcaggccgc
       61 cgcgacgata gcgcgactcc aggcgggcgg tttcgcgcag ctgagtgttg aggcggtagg
      121 cttcgccgcg gctcagcgag ccattgcgca ggccctggtc gatccggcgc tccagcatgg
      181 cctgacgctg attgatggcc ggggcggacg caactgcggg gataacgctg gcggcgccga
      241 gggccgagcc cgccgtgagc gaaagcagga tcttgttcat cggtcggttc ctctcaaaga
      301 tatttccgtc gcccgtgtga cgacgttgac ctcaagaaac cacgcgccgc ctgaagcggt
      361 tctgagccgc tcgttgtctg gcgttcaggt ttggaaaact ctggagggcg cttcaggccg
      421 cgtcggcgag gcgcgcggcg gtcatggcgg cctcgatggc cgcatagagc ttggtgacct
      481 cgatcggctt ggcgacgtga ccgtccatgc cggcggcgag gtagccctcg acctgatggg
      541 ccagggcgtt ggccgtgacc gccacgatcg gggtgcggcg tcgaccttcg gcgcgttcca
      601 gcgcgcggat ggtctgggcg gcctcgacac cgtccatgac cggcatgtgg atatccatca
      661 ggataatgtc gtgcctgccg cgcttccagg cttcgacgca ggccgcgccg tctgcgacca
      721 gctcgacctc ggcgcccagc ggagccagaa cggcggcgat gaccttctga ttggtcggat
      781 tgtcgtcggc ggccagcacg cgaggacggt tgctgacctt gggctggaca tcctcgacga
      841 cggcggcggc cttggtcatc tcgccgccgc ggggtatggc cagcacgaca gtaaaggtcg
      901 agccttggcc ttcgcggctc tgtaccgcga tgccgccgcc catcagggtg gccagttcgc
      961 ggcagatggc caggcccagg cccgatccgc caaactgccg ggttgtcgag ctgtcggcct
     1021 gcacgaactt gtcgaacagg cgcggctgca tctcggcggc gatgccaatg cccgtgtcgg
     1081 cgacgtcgat ccgcagaccc gagccgtcct c
//
LOCUS       DAOMEO010001233         1103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13241_length_1103_cov_2.264313, whole genome shotgun sequence.
ACCESSION   DAOMEO010001233 DAOMEO010000000
VERSION     DAOMEO010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13241_length_1103_cov_2.264313"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLH31_20025"
     CDS             <1..668
                     /locus_tag="PLH31_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQR91513.1"
                     /translation="APPTLPLQSNPHMHLFEASLAWAEQDGDDPEWRDLADMIAELAL
                     SRFIAADTGALREFFDDKWGSAPGVEGRIWEPGHQYEWGWLLLRWGKLAARPDATAAA
                     LRMIDLAERVGCDAERGVAVNAMLDDMSAHDNGARLWPQTERIKAGVLAAEITGDDRW
                     WGVAVEGAEGLLQYLRTPVPGLWRDKLKPDGAFIEEAAPASSFYHIVCAIAELDRAVT
                     AAS"
     gene            complement(669..>1103)
                     /gene="nadE"
                     /locus_tag="PLH31_20030"
     CDS             complement(669..>1103)
                     /gene="nadE"
                     /locus_tag="PLH31_20030"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673835.1"
                     /note="catalyzes the formation of nicotinamide adenine
                     dinucleotide (NAD) from nicotinic acid adenine
                     dinucleotide (NAAD) using either ammonia or glutamine as
                     the amide donor and ATP; ammonia-utilizing enzymes include
                     the ones from Bacillus and Escherichia coli while
                     glutamine-utilizing enzymes include the Mycobacterial one;
                     forms homodimers; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQR91514.1"
                     /translation="DLLEDILSTEISPELVPGEQVQATESFVGPYALQDFNLYYLTRY
                     GMSPSKIAFLAWTAWHDASVGGWPVGTPDAARKAYDLPEIRRWLELFLRRFFANQFKR
                     SAMPNGPKISSGGALSPRGDWRMPSDAAADIWLAELQANTPG"
BASE COUNT          170 a          374 c          389 g          170 t
ORIGIN      
        1 ccgcgccacc gaccctgccg ctgcagtcca atccgcacat gcacctgttc gaggccagtc
       61 tcgcctgggc cgagcaggac ggcgacgatc cagaatggcg cgacctggcc gacatgatcg
      121 ccgaacttgc cctgtccagg ttcatcgccg ccgacactgg cgctttgcgc gagttcttcg
      181 acgacaaatg gggctcggcc ccgggggtcg agggtcgcat ctgggagccc ggccaccagt
      241 acgagtgggg ctggctgttg ctgcgctggg gcaagctggc cgctcgtccc gacgccacgg
      301 cggcggcgct gcggatgatc gatctggccg agcgggtcgg ctgcgacgcc gaacgtggcg
      361 tggccgtcaa cgccatgctc gacgacatga gcgcccacga caacggcgcc cgcctttggc
      421 cccagaccga gcgcatcaag gccggcgtcc tggctgccga gatcaccggc gacgatcgct
      481 ggtggggcgt ggcggtcgag ggggccgagg gtctgctgca gtatctgcgc acgcccgtgc
      541 cgggcctgtg gcgcgacaag ctgaagcccg acggcgcctt catcgaggaa gccgccccgg
      601 cgtcctcgtt ctaccacatc gtctgcgcca tcgccgagct ggacagggca gtcacggccg
      661 ccagctgatc agcctggcgt gttggcctgc agttccgcca gccagatgtc ggcggcggcg
      721 tctgacggca tccgccagtc gccgcgcggc gacagggccc cgcccgacga gatcttcggg
      781 ccgttgggca tggccgagcg cttgaactgg ttggcgaaga accgacgcag gaagagctcc
      841 agccagcgcc tgatctcggg caggtcatag gccttgcgcg ccgcatccgg cgtgccgact
      901 ggccagccgc cgacgctcgc atcgtgccag gcggtccagg ccaggaaggc gatcttggac
      961 gggctcatgc cgtagcgggt caggtagtag agattgaagt cctgcagggc gtaggggcca
     1021 acgaagctct cggtggcctg cacctgctcg cccggcacca gttcgggcga gatctcggta
     1081 ctgagaatgt cttccagcag gtc
//
LOCUS       DAOMEO010001234         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13278_length_1101_cov_1.959847, whole genome shotgun sequence.
ACCESSION   DAOMEO010001234 DAOMEO010000000
VERSION     DAOMEO010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_1101_cov_1.959847"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1101
                     /locus_tag="PLH31_20035"
     CDS             <1..>1101
                     /locus_tag="PLH31_20035"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006275306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HQR91515.1"
                     /translation="AVKFYTQQGNFDLVGNNIPVFFIQDAIKFPDLIHAAKQDPDREF
                     PQAQTAHDTFWDYISLTPESMHMVMWIMSDRTIPRSYRMMEGFGVHTFRLVNAAGAST
                     FVKFHWRPKLNAQSVVWDEAVKINGADPDFHRRDLWEAIDTGDFPEWQFCLQTFSQEE
                     ADALPFDVLDPTKVIPEEVIPLRVVGRMVLDRNPDNFFAETEQVAYCASHVVPGIDFT
                     NDPLLQGRLFSYQDTQLKRLGSPNFHQIPINQPKCPFHNLQRDGHMRMDVPKGQVNYE
                     PSSLGPDVARESQASGYRTFPNLETGEQLRVRPEAFGDHYTQARMFFFSQTKPEQDHI
                     VAALIFELSKCDVARVREAMLARLINIDQTLAL"
BASE COUNT          211 a          370 c          316 g          204 t
ORIGIN      
        1 gcggtcaagt tctacaccca gcagggcaat ttcgatctcg tcggcaacaa catcccggtg
       61 ttcttcatcc aggacgccat caagttcccg gatctgatcc acgcggccaa gcaggatccc
      121 gatcgcgagt ttccgcaggc ccagaccgcc cacgatacct tctgggacta catctcgctg
      181 acgcccgaga gcatgcacat ggtcatgtgg atcatgtccg accggacgat cccgcgcagt
      241 taccgcatga tggagggctt cggggtccac accttccgtc tagtcaacgc ggcgggcgcc
      301 tcgaccttcg tcaagttcca ctggcggccc aagctgaacg cccagtcggt ggtctgggac
      361 gaggccgtga agatcaatgg cgctgatcca gacttccacc gccgggacct gtgggaggcc
      421 atcgatacgg gcgacttccc tgagtggcag ttctgtctgc agaccttcag ccaggaggag
      481 gccgacgccc tgcccttcga cgtgctggat ccaaccaagg tcattcccga ggaagtcatc
      541 ccgctgcgcg tcgtggggcg catggttctg gaccgcaacc cggacaattt cttcgccgag
      601 accgaacagg tggcctattg cgcctcgcat gtcgttcctg gcatcgactt caccaatgac
      661 ccgctgctgc aggggcgcct gttctcttac caggacaccc agctgaaacg ccttggctcg
      721 cccaatttcc accagatccc gatcaaccag cccaagtgcc cgttccacaa cctgcagcgc
      781 gacggccata tgcgcatgga tgttccaaag gggcaggtga actatgagcc gtccagcctg
      841 ggtcccgatg tggcgcgcga gtctcaggcg agcggctaca ggaccttccc caacctcgag
      901 acgggggagc agctcagggt gcgacccgag gccttcggcg accattacac ccaggcccgg
      961 atgttcttct tcagccagac caagcctgag caggaccaca tcgtcgcggc cctgatcttc
     1021 gagctttcca aatgcgacgt ggcgcgtgtg cgcgaggcga tgctggcccg gctgatcaat
     1081 atcgaccaga ccctggccct g
//
LOCUS       DAOMEO010001235         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13290_length_1100_cov_3.332057, whole genome shotgun sequence.
ACCESSION   DAOMEO010001235 DAOMEO010000000
VERSION     DAOMEO010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13290_length_1100_cov_3.332057"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1100
                     /locus_tag="PLH31_20040"
     CDS             <1..>1100
                     /locus_tag="PLH31_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQR91516.1"
                     /translation="AEGAQSKKAPAALTADSTPLPEFEPLTGVRKILFTGVGGTGVTT
                     VASILAMAAHIDGRSGSVVDMTGLAQKGGSVFSHVKIGETEDTVVGGRVPAASTDVLI
                     ACDLLVAASPEGLSLYAKDRTRAFGNSDFAPTADFVTSRDVKFDSSAMARRVKGATKS
                     FDACPAQHLAESQFGDAIFANMIMVGFAWQRGVIPLSSRALYRAIKLNGVEADANLQA
                     FELGRRIAHDPSAAGPKVEAVATPETMSLDDLIAKRTAELTAYQNAAYAQRYAAKVAK
                     VRSAEIAVSGPSGDLADALPLTRAAAVNLYKLMAYKDEYEVARLYTDGRFAAELAGTF
                     KGGKARVWLAPPIIGAKGPDGKPKKIAFGGWML"
BASE COUNT          181 a          388 c          366 g          165 t
ORIGIN      
        1 gccgagggcg cgcagtcgaa aaaggccccc gccgccctga cggccgactc cacgcctctg
       61 cccgagttcg agccgctgac cggcgtgcgc aagatcctgt tcacgggtgt gggcggcacc
      121 ggcgtgacca ccgtcgcctc gatcctggcc atggccgcgc acatcgacgg gcgctcgggc
      181 agcgtggtcg acatgacggg tctggcccaa aagggcggct cggtgttcag ccacgtcaag
      241 atcggcgaga ccgaggacac cgtggtcggc ggccgcgtgc ccgccgccag caccgacgtg
      301 ctgatcgcct gcgacctgct ggtcgccgcc tcgccggaag gcctgtcgct ttacgccaag
      361 gaccgcacgc gagccttcgg caacagcgat tttgctccca ccgccgactt cgtcaccagc
      421 cgcgacgtca agttcgactc aagcgccatg gctcgccggg tgaagggtgc gaccaagagc
      481 ttcgacgcct gtccggccca gcacctggcc gagagccagt tcggcgacgc gatcttcgcc
      541 aacatgatca tggtcgggtt cgcctggcag cgcggggtga tcccgctgtc cagccgcgcg
      601 ctctatcgcg ccatcaagct gaacggggtc gaggccgacg ccaacctgca ggccttcgag
      661 ctgggtcgcc gcatcgcgca tgatccttcg gccgcgggtc ccaaggtcga agcggtcgcc
      721 acgcccgaga cgatgtcgct ggatgacctg atcgccaagc ggaccgccga gctgacggcc
      781 tatcagaacg ccgcctacgc ccagcgctac gccgccaagg tggccaaggt ccgctcagcc
      841 gagatcgctg tcagcggccc aagtggcgat cttgccgacg ccctgccgct gacccgcgcg
      901 gcggcggtca atctctacaa gctgatggcc tacaaggacg agtatgaggt tgcccgtctg
      961 tatacggatg gtcgcttcgc ggcggagctg gccgggacct tcaagggcgg caaggccagg
     1021 gtctggctgg ccccgccgat catcggcgcc aaggggcccg acggcaaacc caagaagatc
     1081 gcctttggcg gctggatgct
//
LOCUS       DAOMEO010001236         1100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13292_length_1100_cov_3.053589, whole genome shotgun sequence.
ACCESSION   DAOMEO010001236 DAOMEO010000000
VERSION     DAOMEO010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13292_length_1100_cov_3.053589"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..>1100)
                     /locus_tag="PLH31_20045"
     CDS             complement(40..>1100)
                     /locus_tag="PLH31_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQR91517.1"
                     /translation="TVALVIGLVVGWLVKPGAGFNIDPATLDPGIATGYLAKAHEGEG
                     MAAYLLHLIPDTFFGAFADGNLLQVLVIAILTGFACTRMGDFGDRVADVLEDVAKLFF
                     GIIHVVVRLAPLGAFGAMGFTIGKYGLEALVQLGALVATFYLTALIFVLVVLGGIAAA
                     AGFSIFRFMAYIREELLIVLGTSSSESVLPQMMEKLERLGARKSVVGLVIPTGYSFNL
                     DGTNIYMTLATLFLAQATNTHLDLWQILSLLGVAMLTSKGASGVTGAGFITLAATLAV
                     VPDIPIAALAVLVGVDRFMSECRALTNLVGNGVATLVVARWDGALDREQLARELQRGP
                     ASTTVLVDGEDAESRHQG"
BASE COUNT          204 a          385 c          356 g          155 t
ORIGIN      
        1 ggtgctggcc accgcggcct atgtggcgat cagagcgctc tatccctgat ggcgcgattc
       61 ggcgtcttcc ccgtcaacca ggacggtggt cgaggccggc ccccgctgca gttctcgcgc
      121 cagctgctcg cgatccagcg cgccgtccca gcgggccacc accagggtcg ccacgccgtt
      181 gccgaccaga ttggtcaggg cccggcactc gctcatgaac cggtcaacgc ccaccagcac
      241 ggccagggcg gcgatcggga tatccggcac gacggccagg gtggcggcca gggtgatgaa
      301 ccccgccccg gtcacgccgc tggcgccctt ggacgtcagc atggccaccc ccagcaggct
      361 cagaatctgc cagagatcca gatgggtgtt ggtcgcctgg gccaggaaca gggtggccag
      421 ggtcatgtag atattggtgc cgtccagatt gaagctgtag ccggtcggga tcaccagtcc
      481 gaccaccgac tttcgggcgc ccagccgctc cagcttctcc atcatctggg gcaggaccga
      541 ctcggacgaa ctggtgccca gcacgatcag cagttcctcg cggatatagg ccatgaacct
      601 gaagatcgag aacccggccg ccgccgcgat gccgcccagc accaccagca cgaagatcag
      661 ggcggtcaga tagaaggtcg ccaccagcgc gccaagctgc accagcgctt ccaggccgta
      721 cttgccgatc gtgaagccca tggcgccgaa ggcgcccagc ggcgcgagcc gcaccaccac
      781 atggatgatt ccgaagaaca gcttggccac gtcctccagg acatcggcca cgcggtcgcc
      841 gaagtcgccc atccgcgtgc aggcaaagcc cgtcaggatc gcgatcacca gcacctgcag
      901 cagattgccg tcagcgaagg ccccgaagaa ggtgtcgggg atcaggtgca gcagataggc
      961 ggccatgccc tcgccctcgt gggccttggc cagatagccg gtggcgatgc cgggatccag
     1021 cgtcgccggg tcgatgttga agcccgcgcc gggcttgacc agccagccga cgaccaggcc
     1081 gatcaccagg gcgacggtcg
//
LOCUS       DAOMEO010001237         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13341_length_1097_cov_2.190019, whole genome shotgun sequence.
ACCESSION   DAOMEO010001237 DAOMEO010000000
VERSION     DAOMEO010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13341_length_1097_cov_2.190019"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLH31_20050"
     CDS             complement(<1..168)
                     /locus_tag="PLH31_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR91518.1"
                     /translation="MIVFLLSALDQTIVATAMPRIVAQLSGLDLYPWVTTAYLLASTV
                     MVPIYGKLSDIY"
     gene            complement(334..>656)
                     /locus_tag="PLH31_20055"
     CDS             complement(334..>656)
                     /locus_tag="PLH31_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91519.1"
                     /translation="QTEIAPDDLASFRRQLVLKSLSAICELPNAARAKVMEGVAPADL
                     FCRIGALSGDGALEAFDAIVWAIAPVPETSDDRALPLILDALAKGDAARARRIFLNHE
                     QAHL"
     assembly_gap    657..666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<667..1072)
                     /locus_tag="PLH31_20060"
     CDS             complement(<667..1072)
                     /locus_tag="PLH31_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR91520.1"
                     /translation="MAGDVAALFDLRRRQLAIIQDSQDAWSAIDRDSRLVPEIETEAS
                     TEAGPGQNPAERLVRELLRDVRLLGWSPGARIGGNRDLLARYGVTVGTLRQAVRMLEQ
                     NSVVQMGRGRSGGLVVTLPDPAKAIDRALDYLR"
BASE COUNT          210 a          352 c          359 g          166 t
ORIGIN      
        1 gtagatgtcc gacagcttgc cgtagatcgg caccatcacc gtcgaggcca gcaaataggc
       61 ggtcgtcacc cacggataga gatccagccc cgacaattgc gccacgatcc gcggcatggc
      121 cgtggcgacg atggtctggt cgagcgccga cagcagaaaa acgatcatca aggcgatcag
      181 cgtcacccgc cgctcaagag gcgagaagtc ttgcggttgc gcgtcgactg acggatcagg
      241 cgcaaggggg ggagcctgcg tcgttgcggg aaatcatagc ttgaaactcg aagggtcagg
      301 gtttgaggaa ccgcgggcgc ggaccactac gggtcagaga tgcgcctgct cgtggtttag
      361 gaagatgcgc cgcgcgcgcg ccgcgtcacc cttggcgaga gcgtcgagga tgagaggaag
      421 cgccctgtcg tcggaggttt caggaaccgg ggcgatcgcc cagacgatag cgtcgaacgc
      481 ctcaagcgcg ccgtcgccgg acagtgcgcc aatgcggcaa aacagatccg cgggcgcgac
      541 gccctccatg actttcgcgc gagccgcatt tggcagctcg cagatcgcgc tcagcgactt
      601 gagcaccaat tgccggcgaa aggacgccag atcatcgggc gcgatttccg tctggcnnnn
      661 nnnnnnggcg caaatagtcc agcgcgcgat cgatggcctt ggcggggtct ggcaaggtga
      721 cgaccagacc gcccgatcgc ccgcgcccca tctgcacgac cgagttctgc tccagcatgc
      781 ggacagcctg gcgcagcgtg cccacggtga cgccatagcg ggccagcaaa tcccggttcc
      841 caccgatccg ggcgcccggc gaccagccga gcagacgcac gtcccggagc aactctcgaa
      901 cgagccgttc ggcggggttc tggccgggtc ctgcttcggt cgacgcctcg gtctcgattt
      961 cgggcactag gcggctgtcg cggtcgatcg cgctccaagc atcctgactg tcctggataa
     1021 tggccagctg ccgacgccgc agatcgaaca aggcggcgac atcaccggcc atctgggcct
     1081 cgaccatctc cagcagt
//
LOCUS       DAOMEO010001238         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13358_length_1096_cov_2.155620, whole genome shotgun sequence.
ACCESSION   DAOMEO010001238 DAOMEO010000000
VERSION     DAOMEO010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13358_length_1096_cov_2.155620"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLH31_20065"
     CDS             complement(<1..240)
                     /locus_tag="PLH31_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HQR91521.1"
                     /translation="MRLFMVLPVLAGGLLLASCATISEEACLQGDWAGIGFKDGEAGH
                     PQSRLDEHAKACAKTGVAPDPAPYFAARAQGLRLYC"
     gene            complement(324..896)
                     /locus_tag="PLH31_20070"
     CDS             complement(324..896)
                     /locus_tag="PLH31_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQR91522.1"
                     /translation="MNILNGKQHRNRARAGREAFEPEVHHETHPATQIALGALLAGGA
                     ILTALAVGRRHEAVLDDDMYAVEFAEMHEPVAHQPKPLTSLILPPLFIAMTLSGLRTW
                     NAPASPARTRALTLWSLTQGFNALWLGLGAKRLGGQVTAATASLAASTAYAIEARKVG
                     GGTAPDLGWLGVANALTAQLWRRPQGPTLH"
BASE COUNT          178 a          374 c          377 g          167 t
ORIGIN      
        1 gcaatagagc ctcagcccct gggcgcgggc cgcgaaatag ggcgcggggt cgggcgcgac
       61 gccggtcttg gcgcaggcct tggcgtgctc atcaagacgg ctctgcggat gcccggcttc
      121 gccgtccttg aagccgatgc cggcccagtc gccctgcaga caggcctctt cactgatcgt
      181 cgcgcagctg gccaacagca ggccgccagc cagaaccggt aacaccatga acagacgcat
      241 gacaaaactc ccccaggaca ttgtccagga gctaaagctt ggccgtatga accgtcaatg
      301 aactgcggag cggcaggcgc ggtctagtgc agggtaggac cctggggccg ccgccacagc
      361 tgggccgtca gggcgttcgc cacccccagc cagcccagat ccggcgccgt gccgccaccc
      421 accttgcgcg cttcgatcgc ataggccgtg gaggccgcca gcgaagcggt cgccgcggtg
      481 acctggccgc ccagacgctt ggcgcccagg cccagccaca gggcgttgaa gccctgggtc
      541 aggctccaca gcgtcagcgc ccgggtgcgg gcggggctgg ccggcgcgtt ccaggtgcgc
      601 agacccgaca gggtcatggc gatgaacagc ggcggcagga tcaggctggt cagcggcttg
      661 ggctgatggg cgacaggctc gtgcatctcg gcgaactcga cggcgtacat gtcgtcgtcc
      721 agcaccgctt catgtcgccg gcccacggcc agagccgtca ggatggcgcc gccggccagc
      781 agggcgccca gcgcgatctg ggtggcaggg tgggtctcgt ggtgcacttc gggctcgaag
      841 gcctcgcgtc cggcgcgggc ccgattccgg tgctgcttgc cattcaggat gttcatgccc
      901 ggactcctcg ctaacctggc tgcctaactg ttcggaacag gcttggttcc gaggcgacag
      961 cgccggaccc atgatggatt tgatcgcctc gcaaagccag cgccctgatc agcggttgcg
     1021 cggcgtgcgc agcagaccat cgatcagggc ctccatggcg ccgagattga gcaacgccgc
     1081 ctgccggatt tcctcg
//
LOCUS       DAOMEO010001239         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13366_length_1096_cov_1.602305, whole genome shotgun sequence.
ACCESSION   DAOMEO010001239 DAOMEO010000000
VERSION     DAOMEO010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13366_length_1096_cov_1.602305"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..798
                     /locus_tag="PLH31_20075"
     CDS             118..798
                     /locus_tag="PLH31_20075"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HQR91523.1"
                     /translation="MVRTLSNDNRQSTETEMIEVYAFATPNSVKVPIALEEMGLAYAL
                     NPVNVRKGEQKETAFVALNPNGKVPVLVDDVEDERFVLTESAAILVHLAEKTGKLLPR
                     DAAGRARVFEQLFFHASVLSPAFGNAGFFKRSSPEPQPIAEARFTGEAERILGLLDAK
                     LVDQAFAAGDDFTIADIAHFGWLWRRQFPGLTLDARPNLSRWYDNVAARPAVQRAIAR
                     VEALVPAA"
     gene            844..>1096
                     /locus_tag="PLH31_20080"
     CDS             844..>1096
                     /locus_tag="PLH31_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91524.1"
                     /translation="MSNFDAYKDSFANARLTRSESGVLEVALHTEGGKLVFNGHTHEQ
                     FVELFHQIGSGADNRVVILTGSGDAFMDAISPEGFDFFSP"
BASE COUNT          209 a          372 c          305 g          210 t
ORIGIN      
        1 ggaacccgtc tctaccaccc gtacgaaagc ggccatcgca tgcagtctgt ccatattgct
       61 tattctctat aggaataggc aatatcacgt caaggcttct gccacaatgc gctgggaatg
      121 gtcagaactc tgtccaacga caacagacag tcgacggaga cagagatgat tgaagtttac
      181 gccttcgcca cgcccaacag cgtcaaggtg ccgattgcgc tggaagagat ggggctggcc
      241 tacgcgctga acccggtcaa cgtccgcaag ggcgagcaga aggaaacggc cttcgttgct
      301 ctcaacccga acggcaaggt gccggtgttg gtcgatgatg tggaggacga acggttcgtc
      361 ctgacggaaa gcgccgccat cctcgtccat ctcgcggaga agaccggcaa actgctcccc
      421 cgcgacgccg cgggccgggc ccgtgtcttt gagcagctct tcttccatgc ctcggtcttg
      481 agccccgcct tcggcaatgc cggtttcttc aaacgttcgt cgccggagcc gcagccgatc
      541 gccgaggccc gcttcaccgg cgaggcggaa cgcatcctcg gtcttctcga tgcgaagctg
      601 gtcgatcagg ccttcgccgc tggtgatgat tttacgatcg ccgacatcgc ccactttggc
      661 tggctgtggc gtcgccagtt tccaggcctg acgctcgacg cccgcccgaa cctctcccgc
      721 tggtacgaca acgtcgcggc gcgcccagcc gtccagcgtg ccatcgcccg cgtcgaggcc
      781 ctcgtgcccg ccgcctgatc cttcccgaac atcgatccaa ttcaagacaa caggagaacc
      841 atcatgtcca acttcgacgc ctacaaagat tccttcgcca acgcccgctt gacccgctcg
      901 gaaagcggcg ttcttgaagt cgcgctgcac accgagggcg gcaagctcgt cttcaacggc
      961 catacccatg agcagttcgt cgagctcttc caccagatcg ggtcgggcgc cgacaaccgc
     1021 gtcgtcatcc tgaccggctc aggcgacgcc ttcatggatg cgatcagccc cgagggcttc
     1081 gatttcttct cgccgc
//
LOCUS       DAOMEO010001240         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13368_length_1096_cov_1.541787, whole genome shotgun sequence.
ACCESSION   DAOMEO010001240 DAOMEO010000000
VERSION     DAOMEO010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13368_length_1096_cov_1.541787"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLH31_20085"
     CDS             complement(<1..299)
                     /locus_tag="PLH31_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HQR91525.1"
                     /translation="MAMAEPQDPKKGDKSKVSAEGRRGGGWLSRIAPGVRGAFAKRET
                     PENLWVKCPDTGEMIFRSDLEAALWVTPAGRHMRIGPEARFKFTFDDGVYETLPTP"
     gene            complement(304..>593)
                     /locus_tag="PLH31_20090"
     CDS             complement(304..>593)
                     /locus_tag="PLH31_20090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQR91526.1"
                     /translation="VTGVKSADAADVAPAVERLRKASGLPVAVGFGIRTPEQAAAVAR
                     VADAAVVGSALVDEIESAAQLNENVTERVLLKASELAKAVQSARVGNEVRL"
     assembly_gap    594..603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<604..1094)
                     /gene="trpA"
                     /locus_tag="PLH31_20095"
     CDS             complement(<604..1094)
                     /gene="trpA"
                     /locus_tag="PLH31_20095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQR91527.1"
                     /translation="MTKDRIDRRFAALKAENRAGFVTYVMAGDPDAATTLSVLKGLPG
                     AGADLIELGFPFSDPMAEGPTIQRAAQRGLKSGMTLQGTLDLVAAFREGDQDTPVILM
                     GYLNPVLNRGFDSFAAAAAKAGVDGLIIVDCPPEEADPLSDALEAEGIALIRLAAPTT
                     DDAR"
BASE COUNT          171 a          364 c          379 g          172 t
ORIGIN      
        1 ggggtgggca gggtctcata gacgccgtcg tcgaaggtga acttgaagcg ggcttcaggg
       61 ccgatccgca tgtggcgacc agccggggtt acccacaggg cggcctccag gtcggagcgg
      121 aagatcattt cgcccgtatc gggacatttg acccagaggt tctcgggcgt ctcgcgcttg
      181 gcgaaggcgc cgcgcacgcc gggggcgatt cgcgaaagcc acccgccgcc gcgtcggcct
      241 tcagccgaga cctttgattt gtcgcccttt ttcgggtcct ggggttcagc catagccata
      301 gccttaaagc ctcacttcgt tcccgactcg cgccgactgc acagccttag ccagctccga
      361 cgccttgaga agaaccctct cggtcacgtt ttcgttcaac tgggcggcgg actcgatttc
      421 atccacaagc gccgagccga ccacggccgc gtcggcgacg cgggccacgg cggcggcctg
      481 ctcgggggtg cggatgccga aaccgaccgc gaccggcaga cccgacgcct tgcgcaggcg
      541 ttccacggcg ggggccacat cggcggcgtc ggccgacttc acgcccgtca cgcnnnnnnn
      601 nnncgggcgt cgtccgtcgt gggggcggcc agacggatca gggcgatgcc ctcggcctcc
      661 agggcgtcag atagcgggtc ggcctcttcg ggcgggcagt cgacaatgat caggccatcg
      721 acccccgcct tggcggcggc ggcggcgaag ctatcaaagc cccggttcag cacggggttg
      781 agatagccca tcaggatgac cggggtgtcc tggtcgccct cgcggaaggc ggcgaccaga
      841 tccagcgtgc cctgcagggt catgccggac ttcaggccgc gctgggcggc gcgctggatg
      901 gtcgggccct cggccatcgg atcggagaag ggaaagccca gctcgatcaa atcagcgccg
      961 gcccccggca ggcccttgag cacggaaagc gtcgtggcgg cgtcgggatc gccggccatc
     1021 acataggtca cgaagccggc gcggttctcg gccttcagcg ccgcgaagcg acggtcgata
     1081 cggtctttgg tcacgc
//
LOCUS       DAOMEO010001241         1095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13377_length_1095_cov_2.442308, whole genome shotgun sequence.
ACCESSION   DAOMEO010001241 DAOMEO010000000
VERSION     DAOMEO010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13377_length_1095_cov_2.442308"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLH31_20100"
     CDS             <1..465
                     /locus_tag="PLH31_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQR91528.1"
                     /translation="GAIVTCDLISPQASALDELKRLAPFIDYFMPSAAEAKALTGQDD
                     LFAAADVFRGLGIGGCLIKNGGQGSVMVLDDIRTTLPAYAITPVDTTSCGDSYCSGFI
                     AALDRGWPPLEAARFATATSALVAQGLATLGKLESFEATELAMGQMTLNGAG"
     gene            465..>1095
                     /locus_tag="PLH31_20105"
     CDS             465..>1095
                     /locus_tag="PLH31_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQR91529.1"
                     /translation="MAAMDEGALRVRLAQVMKAMDERGLNRGTSGNVSARCGAAMLVT
                     PSGVPASQLTPEQMVLVQPDGSTAPGALKPSSEWRMHQGLLDRRPDINAVVHCHSRHA
                     TILACAGRPIPAVHYMVAVSGGTSVPVAPYATFGSSELAEAVVEALDGRYAALMANHG
                     QIVVAPSLDFALAIAEEIEEQAAVYWGTLAIGGPTLLAEDEMHTILQRFK"
BASE COUNT          163 a          379 c          381 g          172 t
ORIGIN      
        1 ggcgcgatcg tgacctgcga cctgatctcg ccccaggcct cggcgctgga cgagctgaag
       61 cgcctggcgc ccttcatcga ctatttcatg cccagcgcgg ccgaggccaa ggccctgacc
      121 ggccaggacg acctgttcgc cgccgccgat gtgtttcgcg ggctggggat cggcggctgc
      181 ctgatcaaga acggcggcca gggctcggtg atggtgctgg acgatatccg caccaccctt
      241 cccgcctacg ccatcacgcc ggtggacacc accagctgcg gcgactccta ttgctcgggc
      301 tttatcgcgg ccctcgatcg cggctggccg cctctggagg ccgcgcggtt cgccacggcg
      361 acctccgccc tcgtggccca ggggctggcg acgctgggca agctggaaag cttcgaggcc
      421 accgaactgg cgatgggcca gatgaccttg aacggagcgg gctgatggcg gcgatggacg
      481 agggcgccct gcgggttcgc ctggcccagg tgatgaaggc catggacgag cggggcctca
      541 atcgcggcac gtcgggcaat gtctcggccc gttgcggcgc cgccatgctg gtgacgccca
      601 gcggtgtgcc ggccagtcag ctgacgcccg agcagatggt gctggtccag cccgatggct
      661 ctaccgcccc gggggcgctg aagccctcca gcgaatggcg gatgcatcag gggctgcttg
      721 atcgacggcc cgacatcaac gccgtggtcc attgccactc gcgccacgcc accatcctgg
      781 cttgcgcggg ccggccgatc ccggcggtgc actacatggt cgccgtcagc ggcgggacca
      841 gcgtgccggt cgcgccctac gccaccttcg gatcgtcgga gctggcggag gccgttgtcg
      901 aggcgctgga cggccgctac gccgccctga tggccaatca cggccagatc gtcgtggcgc
      961 ccagcctgga tttcgccctg gccatcgccg aggagatcga ggagcaggcg gcggtctatt
     1021 ggggaaccct ggccataggc ggtccaaccc tgctggcgga agacgagatg cacaccatcc
     1081 tgcagcgctt caaat
//
LOCUS       DAOMEO010001242         1095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13378_length_1095_cov_2.393269, whole genome shotgun sequence.
ACCESSION   DAOMEO010001242 DAOMEO010000000
VERSION     DAOMEO010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13378_length_1095_cov_2.393269"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1095
                     /locus_tag="PLH31_20110"
     CDS             <1..>1095
                     /locus_tag="PLH31_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQR91530.1"
                     /translation="CGLPKKMALELFKPFIYARLDAKGLSGTVKQSKRMVEREQPQVW
                     DILEEVIREHPVLLNRAPTLHRLGIQAFEPKLIEGKAIQLHPLVCAAFNADFDGDQMA
                     VHVPLSLEAQLEARVLMMSTNNILSPANGRPIIVPSQDIVLGLYYLSVARDGEPGEGK
                     IFADLGEIEAAMDAGVVSLHAKIKSRFTEVDNDGVARRRVIDTTPGRMKIAALLPRHP
                     QVGHRLIEKALTKKEIGNLIDIVYRHCGQKATVIFADQIMGLGFKEAAKAGISFGKDD
                     IIIPQRKEAIVAETRTLAEEYEQQYADGLITKGEKYNKVVDAWAKATDRVADEMMAEL
                     QMKHKDENGREKEINAIYMMAHSGARGSQA"
BASE COUNT          230 a          353 c          338 g          174 t
ORIGIN      
        1 agtgcggcct gccgaagaag atggctctcg agctgttcaa gccgttcatc tatgcgcgcc
       61 tggacgccaa gggcctgtcg ggcaccgtca agcagtccaa gcgcatggtc gagcgcgaac
      121 agccccaggt gtgggacatc ctcgaagagg tgatccgcga gcacccggtc ctgctgaatc
      181 gcgcgccgac gcttcaccgt ctgggcatcc aggcgttcga gcccaagctg atcgagggca
      241 aggccatcca gctgcatccg ctggtctgcg ccgccttcaa cgccgacttc gacggcgacc
      301 agatggctgt ccacgtgccg ctgtcgctgg aggcgcagct cgaagcgcgc gtgctgatga
      361 tgtcgaccaa caacatcctg tcgcccgcca acggtcgccc gatcatcgtg ccgtcgcagg
      421 acatcgtcct gggtctgtac tatctgtcgg tcgcccgcga cggtgagccg ggtgaaggca
      481 agatcttcgc tgacctcggc gaaatcgaag ccgccatgga cgccggcgtc gtcagcctgc
      541 acgccaagat caagtcgcgc ttcaccgaag tggacaatga tggcgtggcc cgtcgccgcg
      601 tgatcgacac caccccgggc cggatgaaga tcgccgccct gctgccgcgt cacccgcagg
      661 tgggtcaccg cctgatcgaa aaggccctga ccaagaagga aatcggcaat ctgatcgaca
      721 tcgtctaccg ccactgcggt cagaaggcga cggtcatctt tgccgaccag atcatgggtc
      781 tgggcttcaa ggaagcggcc aaggccggca tctccttcgg caaggacgac atcatcatcc
      841 cgcagcggaa agaggcgatc gtcgccgaaa cccgcacgct ggctgaagaa tatgagcagc
      901 agtacgccga cggcctgatc accaagggtg agaagtacaa caaggtcgtt gacgcctggg
      961 ccaaggccac cgatcgcgtc gccgacgaga tgatggccga gcttcagatg aagcataagg
     1021 acgagaacgg ccgcgagaag gaaatcaacg ccatctacat gatggcccac tccggcgctc
     1081 gtggttcgca agccc
//
LOCUS       DAOMEO010001243         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13392_length_1094_cov_2.886429, whole genome shotgun sequence.
ACCESSION   DAOMEO010001243 DAOMEO010000000
VERSION     DAOMEO010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13392_length_1094_cov_2.886429"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="PLH31_20115"
     CDS             <1..>1094
                     /locus_tag="PLH31_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQR91531.1"
                     /translation="AARRESAAEKQLSVSGLSPAQTIAWFGGGQPATAEGAMALASAY
                     QASGRNQDAVDLIRRTWRDEIFEADAQRSMAARFGAMLTADDYARRADILLYGQQGPA
                     TRELVALLPADQQAAARARIAFRQNASNATALFDSLSASDQASPGVVFERAAYLRRRG
                     LDSFALQTVRNFPSPPTEEAAAGIWRERRALVGSALRSGDSQGAYAAAANTGLRVGAD
                     AAEAEFYAGWIALARLKQPDAAARHFAAIAAIGASPITKGRALYWQGRAAEAQGDPIA
                     AQAFYAEGARHITTFYGQLAAEKAGMTELKLDRDPVITQADRARFHGRELVRAARMLA
                     DIGARDQFRSLVLFIDDNLPNAEETALLVD"
BASE COUNT          165 a          417 c          358 g          154 t
ORIGIN      
        1 cgccgcccgt cgcgagagcg ccgccgaaaa gcagctttcc gtctccggcc tcagccccgc
       61 ccagaccatc gcctggttcg gcggcggcca gcccgcgacc gccgaagggg ccatggccct
      121 cgcatcggcc tatcaggcca gcggccgtaa ccaggacgcc gtcgacctga tccgccgcac
      181 ttggcgggat gaaatcttcg aggccgacgc ccagcgcagc atggcggcgc gatttggtgc
      241 gatgttgacc gccgatgact atgcccgtcg cgccgacatc ctgctctatg gacaacaggg
      301 cccagcgacg cgcgagcttg tcgcgctgct gccggccgac cagcaagctg cggcgcgcgc
      361 ccgtatcgcc ttccgccaga acgccagcaa cgccacggcg ctgttcgaca gtctcagcgc
      421 cagcgaccag gcgtcgcccg gcgtcgtgtt tgaacgcgcc gcctatctgc gccgtcgcgg
      481 tctggacagc ttcgccctgc agacggtccg caactttccc tcgcccccga ccgaggaagc
      541 cgccgccggg atctggagag aacgccgcgc gctggtcggc tcggccctca ggtcgggtga
      601 cagccagggc gcctatgcgg ccgccgccaa taccgggctg cgtgtgggcg cggacgccgc
      661 cgaggccgag ttctacgccg gctggatcgc gctggcccgc ctcaagcaac ccgacgcggc
      721 ggcgcgccac ttcgccgcca tcgccgcgat cggcgcctcg ccgatcacca agggccgcgc
      781 cctgtattgg cagggccgcg cggccgaggc ccagggcgac cccatcgcgg cccaggcctt
      841 ctatgccgaa ggcgcccgtc acatcaccac cttctatggc cagctggccg ccgagaaggc
      901 tggcatgaca gagctgaagc tcgaccggga ccccgtcatc acccaggccg accgcgcgcg
      961 tttccatggc cgggaactgg tgcgcgccgc gcggatgctg gccgatatcg gggccaggga
     1021 tcagtttcgc tcgctggtgc tgttcatcga cgacaacctc cccaacgccg aagagacggc
     1081 gctgctggtc gatt
//
LOCUS       DAOMEO010001244         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13410_length_1093_cov_3.080925, whole genome shotgun sequence.
ACCESSION   DAOMEO010001244 DAOMEO010000000
VERSION     DAOMEO010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13410_length_1093_cov_3.080925"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1093
                     /locus_tag="PLH31_20120"
     CDS             <1..>1093
                     /locus_tag="PLH31_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91532.1"
                     /translation="NSGLTVTYANNGGDKPSITTNKDLNDPSMGWVWDGGRVNIQNEK
                     RVTATEGAHFDLTIGDEAGMNFKIGAAYDDVSRRITAIDNSARWQTFTCGGGGVTPQS
                     AVPAPVPGCDGRAGSAVPQASLASYIKPGPIGFVTLNREKFFADTNYDAFNAAAPFSN
                     SAATGAASGFIRESTTGAYIEFNGVTQVLERDLRINGGVRYYNTEQTIEGPQTVNGVT
                     SFLSRVTKYDGMLPSFNAAWNLKDDIILRMSASRTLTRANPSAMLPGTTFSDPSAQQA
                     NQGNPNLQPYLSNNFDIGGEWYTGGAGYVGVALFRKELTGFTISGNNTIPFLQLGVPY
                     DQLTNLQQQAITNRGGPNNATVTVSQQVNA"
BASE COUNT          218 a          404 c          320 g          151 t
ORIGIN      
        1 gaactcgggc ctgacggtga cctacgccaa caacggcggc gacaagccgt cgatcacgac
       61 caacaaggac ctcaacgatc cttcgatggg ctgggtctgg gatggcggcc gcgtcaacat
      121 ccagaacgag aagcgcgtca ccgccaccga aggcgcgcac ttcgatctga cgatcggcga
      181 cgaggcgggc atgaacttca agatcggcgc ggcctatgac gacgtcagcc gccggatcac
      241 cgcgatcgac aacagcgcgc gctggcagac cttcacctgc ggcggcggcg gcgtcacccc
      301 gcagagcgcg gttccggctc cggtccccgg ctgcgacggc cgcgcaggct cggctgttcc
      361 gcaggccagc ctggccagct acatcaagcc cggcccgatc ggcttcgtga ccctgaaccg
      421 cgaaaagttc ttcgccgaca ccaattacga cgccttcaac gccgcggcgc cgttctccaa
      481 ctcggccgcc acgggcgcgg cctcgggctt catccgcgag tcgaccaccg gcgcctatat
      541 cgagttcaac ggcgtcaccc aggtgctgga gcgcgacctg cgcatcaacg gcggcgtgcg
      601 ctactacaac accgagcaga ccatcgaagg tccgcagacg gtgaacggcg tcacctcgtt
      661 cctcagccgc gtcaccaagt acgacggcat gctgccctcg ttcaatgcgg cctggaacct
      721 gaaggacgac atcatcctgc ggatgagcgc ctcgcgcacc ctcacccgcg ccaacccgtc
      781 ggccatgctg ccgggcacca ccttcagcga tccttcggcc cagcaggcca accagggcaa
      841 ccccaacctg cagccctatc tgtcgaacaa tttcgacatc ggcggcgaat ggtacacggg
      901 cggcgcgggc tatgtgggcg tggccctgtt ccgcaaggaa ctgaccggct tcacgatcag
      961 cggcaacaac accatcccct tcctgcaact gggcgtgccc tacgaccagc tgaccaacct
     1021 gcagcaacag gcgatcacca accggggcgg ccccaacaac gcgacggtca cggtcagcca
     1081 gcaggtcaac gcc
//
LOCUS       DAOMEO010001245         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13411_length_1093_cov_2.690751, whole genome shotgun sequence.
ACCESSION   DAOMEO010001245 DAOMEO010000000
VERSION     DAOMEO010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13411_length_1093_cov_2.690751"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..642)
                     /locus_tag="PLH31_20125"
     CDS             complement(298..642)
                     /locus_tag="PLH31_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91533.1"
                     /translation="MTQGLREDLFAELFDNMLTLWEATDFTGCDTWFDRSQRVEAAFG
                     AVEHRIDLVAMNYAVHRAIAPDARFDWPLRQLVDRDEARQSFQANMMRVLMGAAGVWQ
                     KDNLEIARRYFG"
     gene            complement(656..>1093)
                     /locus_tag="PLH31_20130"
     CDS             complement(656..>1093)
                     /locus_tag="PLH31_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQR91534.1"
                     /translation="KVRFEFGAAALTSKVIDGAFPDYLRVIPRDNAKILTLDNDLFAK
                     AVDRVATISAEKSRSVKLAIEPGRITLTVRNMEAGQAVEEVEVDYDGEPFEIGFNARY
                     LLDVCGQIGGPIAEFRFADPASPTLVVDPTDPGVKYVLMPLRV"
BASE COUNT          181 a          352 c          373 g          187 t
ORIGIN      
        1 cccccgccgc cgcaatggtc gctggccttg cgccctttcc tcgcgacgag gtgggtcaag
       61 aatacgggag gttttcaggg ggctgcgtcg attgtctgga cgcccataca agtcctcccc
      121 ctagcggggg aggcgatcgc gtagcgatcg gagggggaag tgctgctgaa tcaatgcggt
      181 attcccccac cggcctccgg ccgcctcccc cttcaggggg aggacttagg gcgtctatcc
      241 ccgtgcctat ccggggacgg cgcgatcccg atccccgtag ctgggccccg cgctcaccta
      301 cccaaaatac cgccgcgcga tctccagatt gtccttctgc cagaccccgg cggcgcccat
      361 cagcacgcgc atcatgttgg cctggaagct ctgacgcgcc tcgtcgcggt cgaccagctg
      421 acggagcggc cagtcgaagc gggcgtccgg cgcgatggcg cggtggacag catagttcat
      481 ggcgaccagg tcgatgcggt gctcgacagc gccaaacgcc gcctcgacgc gctgggagcg
      541 gtcaaaccag gtgtcgcagc ccgtaaagtc ggtcgcttcc cagagggtga gcatgttgtc
      601 gaacagctcg gcgaacaggt cttcgcgcag cccctgggtc atcggccaag cgggatcaga
      661 cccgcagcgg catcagcacg tatttgacgc ccggatcggt cggatcgacc accagggtgg
      721 ggctggccgg gtcggcgaag cggaactcgg cgatgggacc gccgatctgg ccgcagacgt
      781 ccagcaggta gcgggcgttg aaaccgatct cgaagggctc gccgtcgtaa tcgacctcga
      841 cctcttcgac ggcctggccg gcttccatgt tgcgcacggt cagggtgatg cggcctggct
      901 cgatggccag cttcaccgaa cggctcttct cggccgaaat ggtggcgacg cggtcgacag
      961 ccttggcgaa caggtcgttg tccagggtga ggatcttggc gttgtcgcgc gggatgacgc
     1021 gcagatagtc ggggaaggcg ccgtcgatga ctttcgaggt cagggcggcc gcgccaaatt
     1081 cgaagcgcac ctt
//
LOCUS       DAOMEO010001246         1091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13456_length_1091_cov_1.820463, whole genome shotgun sequence.
ACCESSION   DAOMEO010001246 DAOMEO010000000
VERSION     DAOMEO010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13456_length_1091_cov_1.820463"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1082)
                     /locus_tag="PLH31_20135"
     CDS             complement(378..1082)
                     /locus_tag="PLH31_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6118 family protein"
                     /protein_id="HQR91535.1"
                     /translation="MDQELGSEAPADPAAAAFEALRREVALLSVALGGLAAERRTTPD
                     YSETLGEIAQGVSLAVARIATLASSPALSLSPEQMAREITQASEAARLQDRAAVHQAR
                     DILADVARDLRGWIDTARLADLQNLRLAQAAAAGLVVGAVLCATLPALVAQAAPEDWA
                     WPEKRAAGVLKRDMASAGERLLTVADPQGWRAMQTARSIFDDNRAVITRCARTADKAQ
                     KPSRCVILLKPTRRPG"
BASE COUNT          174 a          375 c          351 g          191 t
ORIGIN      
        1 catccttgaa ggcttcctga aaccgctgct cagtgaccgg acccaaaatc acggcctaga
       61 ccctttcatt gtagagatcc gaggcgcacg cgcgtcgcgg ggttgctctg tcgatcgcgc
      121 gacgacccgt ctcaaagtta agagttgtga gccggaagcc tgctgacaag tctttgctga
      181 ccaaatcgtc ctctgggata gaaaaagaaa gtctcggcgg gtttgcgagg gggcggccat
      241 gtgccccggc cctgtgggaa cgtctcagct tggcgagggc aggggtgtcg gtcgagcgct
      301 gccgcgaccc gggcagacgc tgtttggccg cagatgaggc ccgatctcgt tttccgccct
      361 catgccttgg gcacggttca tcctggtcgc ctggtgggtt tgaggaggat cacgcaacgg
      421 cttggctttt gagccttatc ggccgtgcga gcacagcgcg tgatgaccgc ccggttgtcg
      481 tcaaagatcg accgcgccgt ctgcatcgcc cgccaccctt gaggatcagc gaccgtcaac
      541 aaccgctcgc ccgccgacgc catgtcgcgt ttgaggacgc cggccgcacg cttctccggc
      601 caggcccagt cttctggcgc ggcctgggcg acgagcgccg gaagtgtggc gcagagcacc
      661 gcacccacaa ccaggcccgc ggcggcggcc tgcgccagcc gcaggttctg caggtcggcc
      721 agccgggcgg tgtcgatcca accgcgaaga tcgcgcgcca cgtccgcgag gatgtcccgc
      781 gcttgatgca cggccgcgcg atcctgaagg cgcgccgcct cgcttgcctg ggtgatctcg
      841 cgcgccatct gctctggact gagagatagg gccgggctgg acgccagcgt ggcgatcctc
      901 gcgaccgcaa gactgacgcc ctgggcaatc tcgcccaggg tctcgctgta gtcgggcgtc
      961 gtcctccgtt cggcggccag accgcccaag gcgacgctca acaacgcaac ctcccgccgc
     1021 aatgcctcga acgccgccgc cgccggatcg gcgggcgctt ctgatccaag ctcctgatcc
     1081 atcccttcct c
//
LOCUS       DAOMEO010001247         1090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13470_length_1090_cov_2.303382, whole genome shotgun sequence.
ACCESSION   DAOMEO010001247 DAOMEO010000000
VERSION     DAOMEO010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13470_length_1090_cov_2.303382"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLH31_20140"
     CDS             <1..559
                     /locus_tag="PLH31_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQR91536.1"
                     /translation="LVALALEGLTVVRLKGGDPFLFGRGGEELAACREAGVECHVVPG
                     VTAALAASAGAGAPLTHRGSAQAVTFVTGHAAHGDPDLDWVALARPNQTVVVYMGVST
                     AGLIAQRLIAAGRAASTPALVVENASRADERRILTTLAGLAVAAEGLKGPALLMVGEA
                     MAMADVAPVDAVVSQFQSFERSSRS"
     gene            556..918
                     /locus_tag="PLH31_20145"
     CDS             556..918
                     /locus_tag="PLH31_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HQR91537.1"
                     /translation="MKAITANRLLDGEVVFWKSGQWVEAFGDAQLFDNDDAAVDAAVA
                     TGKGQPTVVVDVYPIDLTPSEGHWAPVSYRERIRALGPTNEPTHGKQADGGESIDALR
                     HAAGAARSSGRVDLIRRK"
     gene            922..>1090
                     /locus_tag="PLH31_20150"
     CDS             922..>1090
                     /locus_tag="PLH31_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HQR91538.1"
                     /translation="MYQYDIIDREFLIDRSNEFRGQVQRRLAGEMTEDQFKPLRLMNG
                     LYLQLHAYMLRV"
BASE COUNT          155 a          385 c          371 g          179 t
ORIGIN      
        1 gctggtcgct ctggcgcttg aggggctgac ggtcgtgcgt ctgaagggcg gcgacccgtt
       61 cctgttcgga cgcggcggcg aagagctggc cgcctgtcgc gaggccggcg tcgagtgcca
      121 cgtcgtgcct ggcgttaccg ccgccctggc cgcctcggcc ggcgctggcg cgcccctgac
      181 ccatcgcggt tcggcgcagg ccgtcacctt tgtcaccggc catgccgctc acggggatcc
      241 ggacctggac tgggtcgccc tggcgcgacc caaccagacc gttgtcgtct atatgggggt
      301 ctcgaccgcc ggcctgatcg cccagcgcct gatcgccgct ggtcgtgcgg cctcgacccc
      361 ggcgcttgtc gtcgagaacg ccagccgcgc cgacgagcgc cgcatcctca ccaccctggc
      421 cggtctggcc gtcgcggcgg agggtctgaa aggccctgcg ctgttgatgg tgggcgaggc
      481 catggcgatg gctgacgtcg cgcccgtcga cgctgtcgtt tcccagtttc aatcgttcga
      541 gcggagctcg cgctcatgaa agccattacc gccaaccgcc tgctcgacgg cgaggtcgtg
      601 ttctggaagt ccggccagtg ggtcgaggcc ttcggcgacg cccagttgtt tgacaatgac
      661 gacgccgccg tcgatgccgc cgtcgccacg ggcaagggcc agcccaccgt ggtggtcgat
      721 gtctatccca tcgacctgac cccgtccgag ggccactggg cgccggtcag ctatcgcgag
      781 cgcatcaggg cgctgggccc caccaatgaa cccacccacg gcaagcaggc cgacggcggc
      841 gagtccatcg acgccctgcg ccacgcggcg ggcgcggccc gctccagcgg ccgtgtcgat
      901 ctgatccgca ggaagtaagc gatgtaccag tacgacatca tcgaccgcga gttcctgatc
      961 gaccgctcca acgagttccg gggtcaggtg cagcgccgcc tggccggcga aatgaccgag
     1021 gaccagttca agccgctgcg gctgatgaac ggcctgtatc tgcaactgca cgcctacatg
     1081 ctgcgggtgg
//
LOCUS       DAOMEO010001248         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13486_length_1089_cov_2.699226, whole genome shotgun sequence.
ACCESSION   DAOMEO010001248 DAOMEO010000000
VERSION     DAOMEO010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13486_length_1089_cov_2.699226"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /gene="trpS"
                     /locus_tag="PLH31_20155"
     CDS             complement(<1..480)
                     /gene="trpS"
                     /locus_tag="PLH31_20155"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQR91539.1"
                     /translation="MTDQTAAAYAGPARVLSGVQPSGALHLGNYLGALVKFARLQHEI
                     DTFIFVADLHAITVWQEPAALAQQTREIAAAYLAAGLDPAKATIFPQSAVPEHAELAW
                     IFQCVARLGWLDRMTQFKEKSGKNKERSSVGLYTYPVLQAADILIYKATHVPVGEDQK
                     "
     gene            complement(642..>1089)
                     /locus_tag="PLH31_20160"
     CDS             complement(642..>1089)
                     /locus_tag="PLH31_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HQR91540.1"
                     /translation="FFARGDTKSPMRFALVSVAVNIGLGLALFPVMGVPGLAAATSAA
                     SWINVLQMVLTLGKRGDYTPSLHAWGKIVRVLAASVALGLILAAMSHWRAAIEAPILG
                     LGLASAKELAILATCAVAAGLYPLLLLLSGGVTIAEAKTVLRRGRR"
BASE COUNT          181 a          359 c          398 g          151 t
ORIGIN      
        1 cttctggtcc tcgcccaccg gcacgtgcgt ggccttgtag atcaggatgt cggcggcctg
       61 cagcaccgga taggtgtaga ggccgacgct cgagcgctcc ttgttcttgc cgctcttttc
      121 cttgaactgg gtcatgcggt ccagccagcc cagacgggcc acgcactgga agatccaggc
      181 cagttcggcg tgctcgggca cggccgactg cgggaagatg gtggccttgg ccgggtcgag
      241 accggcggcc agataggcgg cggcgatctc gcgggtctgc tgggccaggg cggccggctc
      301 ctgccagacg gtgatggcgt gcaggtcggc cacgaagatg aaggtgtcga tctcgtgctg
      361 caggcgggcg aacttcacca gcgcgcccag atagttgccc aggtgcaggg cgccggaggg
      421 ttgcacgccg gagagcacgc gggcggggcc cgcataggcg gcggcggttt ggtcagtcat
      481 ggcttcagcc ttggatcttg aaacttggaa tggcgagcgc ggcagcgcga accgcgccga
      541 aacgcggaga tcagggtcag cgggagccgc gccatcgcgg cgcgaaggga ccgatcaaag
      601 ccatggacgc tggttaccgc tccggacgcc gcagggcaag cctagcgccg gccgcgccgc
      661 agcacggtct tggcttccgc gatcgtcacc ccgcccgaga gcagcagcag caacggatag
      721 agccccgccg ccacggcgca tgtcgccagg atcgccagct ccttggcgga ggcgaggccc
      781 agccccagga tcggcgcctc gatggcggcg cgccagtgcg acatcgccgc caggatcagg
      841 cccagggcga cgctggccgc cagcacgcgg acgatcttgc cccaggcgtg aaggctgggg
      901 gtgtagtcgc cgcgcttgcc cagggtcagc accatctgca gcacattgat ccacgaggcg
      961 gcgctggtcg cggcggccag gcccggcacg cccatgaccg ggaacagggc caggcccagg
     1021 ccgatgttca cggccacgga aaccagggca aagcgcatcg ggctcttggt gtcgccccgc
     1081 gcgaaaaac
//
LOCUS       DAOMEO010001249         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13506_length_1088_cov_2.397870, whole genome shotgun sequence.
ACCESSION   DAOMEO010001249 DAOMEO010000000
VERSION     DAOMEO010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13506_length_1088_cov_2.397870"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLH31_20165"
     CDS             complement(<1..217)
                     /locus_tag="PLH31_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HQR91541.1"
                     /translation="MARSDVEGFEIAFHSSLSEPVTIAGVPRMIAVLNGTLTAVLALG
                     LQVPLIGIPLGLAIHGACYWLNKRDPYF"
     gene            complement(217..564)
                     /locus_tag="PLH31_20170"
     CDS             complement(217..564)
                     /locus_tag="PLH31_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HQR91542.1"
                     /translation="MKNAGSSQARGRASTALALLAGAAVLLAVSPAWAAGAGTAMPWE
                     GPLDTIMQSLSGPVAKAVGIIAIVLTGLGFAFAEGGSALRKGIGIVFGLAIAFTATTF
                     ISTFFNMTAGAAF"
     gene            complement(898..>1088)
                     /locus_tag="PLH31_20175"
     CDS             complement(898..>1088)
                     /locus_tag="PLH31_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HQR91543.1"
                     /translation="ADNPERACDDANIIAQEQLMAVESKIAQLEALRAELRRMVATGC
                     QGFASDCRVIETLSGSAS"
BASE COUNT          197 a          337 c          357 g          197 t
ORIGIN      
        1 agaagtaggg gtcgcgcttg ttcagccagt agcaggcgcc gtggatggcg aggccgagcg
       61 gaatcccgat cagagggacc tgcaggccca gggccaagac ggcggtcagg gtgccgttga
      121 gcaccgcgat catgcgcggc acgccggcga tcgtcaccgg ctccgacagc gagctgtgga
      181 aagcgatctc aaacccctcg acgtccgacc gagccatcag aacgccgccc cggcggtcat
      241 gttgaagaag gtggagatga aggtcgtggc cgtgaaagcg atcgccagac cgaagacgat
      301 gccgatccct ttgcgcaggg ccgagccgcc ttcagcgaag gcaaagccca gcccggtgag
      361 cacgatcgcg atgatgccga cggccttggc gaccggtccc gacagcgact gcatgatggt
      421 gtcgagcggg ccttcccagg gcatggcggt tcccgcgcca gcggcccaag ccgggcttac
      481 cgccagaagg actgccgcgc cggcgaggag ggccagggcg gtcgaagcgc gcccccgagc
      541 ttggctagaa ccggcgtttt tcatcgcatc tgacccttga ttgcgtccgc ccgcctttac
      601 cgggcgtcac aaccgtgagt cgcggaatag gcgaggtcaa gggctttttt tgataaaaag
      661 gtgcgattag ggctttttct ctagcaaagc tacaaccctg agaggcgatc ttccggccac
      721 gcttcagcgc cctgaatcga gcaggcaagc gcgcgcattt cccccgtgtt gctggtctgt
      781 ttccaactga taagcggcga ctttgcggca catgcccgag ttgtgtgggc tgatgattcg
      841 gctctgggat ggcgcccgat tcttcgacgg catgacgtcg tctcgctcgg attgtgttta
      901 gctggcgctg ccggacaagg tttcgatcac ccggcaatca ctggcaaacc cctggcaccc
      961 cgtcgcgacc atgcggcgga gttcggcgcg gagcgcctca agttgggcga tcttgctctc
     1021 caccgccatc agctgctctt gggcaatgat gttggcgtcg tcgcaggccc gctcaggatt
     1081 gtccgcca
//
LOCUS       DAOMEO010001250         1088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13508_length_1088_cov_2.008712, whole genome shotgun sequence.
ACCESSION   DAOMEO010001250 DAOMEO010000000
VERSION     DAOMEO010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13508_length_1088_cov_2.008712"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLH31_20180"
     CDS             <1..657
                     /locus_tag="PLH31_20180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR91544.1"
                     /translation="TRLAVGPDRLATWPLFSADPTAPQARPWRPGETLQIGALGRLHP
                     AKGYDVLITALVRLRDSGFSPPCHFEVRLAGEGAERARLQAMIDESDLRNITLPGFCD
                     DPREFLAGLHLYLQPSRREGLCIAAHEAMQAGLPTIVSSVGEMPYSVQHGVTGLVVPP
                     DDAGALAKALRRLLSGPHQLGPMGLAARTRVLERFGEERFAQTGAEIFARLSVAGASR
                     "
     gene            654..>1088
                     /locus_tag="PLH31_20185"
     CDS             654..>1088
                     /locus_tag="PLH31_20185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004615384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR91545.1"
                     /translation="MSLSSWLLRKALTPLIRRGGLRLALPSGERLAFGDMSAPVVARL
                     TDERALWSLLLDPDLRTGELFTDGRLVMEQGSIYDFLSLILDNDGERNPSPIVQAFDR
                     FRTATQLWRQRNGPDRSRRNVAHHYDLGDDFYALFLDPDWQYS"
BASE COUNT          166 a          349 c          343 g          230 t
ORIGIN      
        1 acgcgtctgg ctgttgggcc cgatcgcttg gcgacttggc cacttttctc cgctgatccg
       61 acagcgccgc aggcgcgccc ctggcgcccc ggcgaaacgc tccagatcgg cgctctgggg
      121 cggctacatc cggccaaagg gtatgacgtg ctgatcacgg cgcttgtccg gctgcgcgat
      181 agtggctttt caccgccctg ccactttgag gtgcgtctgg ccggcgaggg cgcagaacgc
      241 gcgcgcctgc aggccatgat cgatgagtcg gacttgagaa atataacctt gccaggcttt
      301 tgcgatgatc cgcgcgaatt cctggcgggc cttcatctct atctccagcc gtcgcgtcgc
      361 gagggtctgt gcatcgccgc gcacgaggca atgcaggcag gactgccgac gattgtctcg
      421 tcagtcggtg agatgcccta cagcgttcag catggcgtta ccggactggt cgtcccgcca
      481 gacgatgccg gcgccttggc caaggctctg cggcgcctgc tctctggccc acaccaattg
      541 ggtcccatgg gcctagcggc acggacccgg gttctcgaac ggttcggtga ggaacgcttt
      601 gcccagaccg gcgccgagat cttcgccagg ctctctgtag ccggggcgtc gcgatgagcc
      661 tttcatcgtg gttgctgcgc aaggcgctga cgccgttgat ccgtcgcggg gggctgcggc
      721 tggctttgcc ctcgggtgaa cgtctggcgt ttggggacat gagtgcgcct gttgttgcgc
      781 gattgaccga tgagcgcgcg ctctggagct tgctactaga tcctgacctg cgcaccggcg
      841 agctatttac cgacggtcgc ttggtcatgg agcaggggtc gatctacgac tttctcagtc
      901 tgatcctgga caatgacggc gagcgaaacc cttcaccgat cgtccaggcc ttcgatcggt
      961 ttcgcacggc gacccagctt tggcggcaac gcaatggccc agaccgatcc cgccgcaacg
     1021 tcgcccacca ctatgatctg ggcgatgact tctatgccct ctttctcgat cccgactggc
     1081 aatattcc
//
LOCUS       DAOMEO010001251         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13531_length_1086_cov_2.918526, whole genome shotgun sequence.
ACCESSION   DAOMEO010001251 DAOMEO010000000
VERSION     DAOMEO010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13531_length_1086_cov_2.918526"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..425)
                     /locus_tag="PLH31_20190"
     CDS             complement(84..425)
                     /locus_tag="PLH31_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HQR91546.1"
                     /translation="MALDPHFPSAPSPGGVTFFERRELDQLMRLYGRMVAAGEWRDYG
                     IAGLSDHAVFSIFRHAAEAPLYRVEKRPALARKQGAWAVIGQGGMILKRGHDLAQVLK
                     VFEKGKFSVVD"
     gene            complement(956..>1086)
                     /locus_tag="PLH31_20195"
     CDS             complement(956..>1086)
                     /locus_tag="PLH31_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HQR91547.1"
                     /translation="PWAGRSKLYRLPPATGDDLETVVIVQGAKGGRILGVLETAER"
BASE COUNT          180 a          379 c          338 g          189 t
ORIGIN      
        1 gcctttacgt tctagagccc tttccgacct gatggcttca tctgggtcgg gcgtttcaca
       61 cgcatcagaa aatgcgccag gcctcagtcc accaccgaga acttgccctt ctcgaacacc
      121 ttcagcacct gggccaggtc gtggccgcgc ttgaggatca tgccgccctg gccgatcacc
      181 gcccaggccc cctgcttgcg agccagggcg gggcgctttt caacccggta gaggggggcc
      241 tcggcggcgt gtcggaagat cgagaacacg gcgtggtcgg agaggccggc aatcccgtag
      301 tcacgccatt cgcccgcggc gaccatgcgg ccatacagac gcatgagctg gtccagctcg
      361 cgccgctcga agaaggtcac gccgccgggc gagggggcgg acggaaagtg cggatccaac
      421 gccatcggac gaatcctaag cctgaatcgg cgcccttggc gagcgtggcg tcgcggccac
      481 gattggcctc aatgctacac gacggtcacg ccgcgccgcg aaaggacctt atttcggtcg
      541 ttcggccgcc tgtttgggcc gcgataaatt cagtgtcggc tgatcggaca cctcgcggtc
      601 caggaccctg aacagccccc ccagcccccc ttgatcgtgg gccggtcggc cgacagacta
      661 tacccctccc aatgagtccg cgcggtcctt ccccccaccg cgcgggcttt ttgctgtctg
      721 gacagcggcg agatcaaagc ttggccaatg gccttgaaac caccttggtt tggtcgcggt
      781 cgcgcctgtt tggcccgcga taaagcaagg gtcagccgac cggcttcggg tcggggccct
      841 gaacagcccc cccagccctc cccggtttcg aaccggtccg ctgacgatca agagcccctc
      901 tccaaaagag cccgcgcgat tgccccctcg gtcgcgcggg tttttgctgt tggggtcagc
      961 gctcggcggt ttcgagcacg ccaaggatcc ggccgccctt cgcgccctgg acgatcacga
     1021 ccgtctccag atcatcaccc gtagccgggg gcaggcggta gagcttggat cgacccgccc
     1081 aggggc
//
LOCUS       DAOMEO010001252         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13532_length_1086_cov_2.876819, whole genome shotgun sequence.
ACCESSION   DAOMEO010001252 DAOMEO010000000
VERSION     DAOMEO010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13532_length_1086_cov_2.876819"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..434)
                     /locus_tag="PLH31_20200"
     CDS             complement(45..434)
                     /locus_tag="PLH31_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQR91548.1"
                     /translation="MREHAYAVYMMTDSRRGVLYVGVTSNLVSRAQQHRDGEVSGFTR
                     QHGCKSLVWFETHEDIAAAIHREKLIKRWRRSWKITLVETRNPEWVDLVPGLLGEERL
                     PWETMAEFQVALEPDAIGLFPRKDPYP"
     gene            complement(441..>1086)
                     /locus_tag="PLH31_20205"
     CDS             complement(441..>1086)
                     /locus_tag="PLH31_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQR91549.1"
                     /translation="PVAKLGDSKKLRRGQLVIAIGAPLGFEATVTTGVVSALGRSLRG
                     ERGRLIEDLIQTDAALNPGNSGGPLVNSAGEVVGIATAVIAGYQGLCFAVAVNTATYV
                     IAELMAHGFVRRGSIGLIAQQAPIPAGLARATGVTQPYAVFVAHVDTGGPAAKAGVKE
                     GDLLICAGDHVLTGLDDLLRALDHHSIGKAILFTLIREGRLMTVSITPRIRRRG"
BASE COUNT          176 a          370 c          361 g          179 t
ORIGIN      
        1 ggtcccggat cggcgcgctg cgcgcttgtc cgggatgaca gtctttaggg gtaggggtct
       61 ttccgtggga acagtccgat cgcgtcaggc tccagcgcca cctggaactc agccatggtc
      121 tcccagggaa gtcgttcctc gcccagcaac cccggaacca gatccaccca ttcaggattg
      181 cgcgtctcga cgagggtgat tttccagctt cgccgccatc gcttgatgag cttctcgcgg
      241 tgtatggccg ccgcgatatc ttcgtgcgtc tcgaaccaga ccagcgattt gcacccatgc
      301 tggcgcgtaa atccgctgac ctcaccgtcg cgatgctgct gcgcgcgaga caccagattt
      361 gacgtcacgc cgacatagag cacgccacgg cgactgtcgg tcatcatgta gacggcatag
      421 gcgtgctccc gcatcgccgc ctaaccccgc cgccggatcc tcggcgtgat cgacaccgtc
      481 atcagccggc cttcgcggat cagggtgaac aggatggcct tgccgatgct gtggtgatcc
      541 agcgcccgca acagatcgtc caacccggtc agcacgtgat cgccggcgca gatcagcagg
      601 tcgccttcct tgacgccggc cttggccgcg ggtccgccgg tgtccacatg ggccacgaac
      661 accgcatagg gctgggtgac gccggtggcg cgggccaggc ccgccgggat cggggcctgc
      721 tgggcgatca ggccgatgga gccgcgccgc acgaagccgt gggccatcag ctcggcgatc
      781 acataggtgg cggtgttcac ggcgacggcg aagcacagcc cctgatagcc ggcgatcacg
      841 gcggtggcga tgcccaccac ctcgccagcc gaattgacca ggggcccgcc gctgttgccg
      901 ggattgaggg cggcgtcggt ctggatcagg tcctcgatca ggcggccgcg ttcgccgcgc
      961 aaggaccggc ccagcgccga gacgacgccg gtggtcacgg tggcctcaaa gcccagcggg
     1021 gcgccgatgg cgatcaccag ctggccgcgc cgcagcttct tggaatcgcc gagcttggcg
     1081 acgggc
//
LOCUS       DAOMEO010001253         1084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13573_length_1084_cov_1.971817, whole genome shotgun sequence.
ACCESSION   DAOMEO010001253 DAOMEO010000000
VERSION     DAOMEO010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13573_length_1084_cov_1.971817"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLH31_20210"
     CDS             <1..999
                     /locus_tag="PLH31_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR91550.1"
                     /translation="VGFDEAAYALSLEGGYEYDTTTVRFVYNSMTTPRQWFDYDMTSR
                     QRTLRKTQEIPSGHDPARYETRRLNAKASDGTEVPIFVLMKKGTRLDGSAPLLLYGYG
                     SYGISMEASFSIRNFSLVDRGWIWATACPRGGSEKGWSWFLDGKKDKKKNTFTDFIAC
                     AEHLHAAGYGKPERTVAYGGSAGGLLMGAITNMRPDLFAGIIAAVPFVDVVNTMSDTT
                     LPLTPPEWPEWGNPLEDAAAYDYIASYSPYDNIAAKPYPAILATGGLSDPRVTYWEPE
                     KWVAKLRPATTSGKPVLLKINMEAGHGGASGRFDFLKEIALDYAFAVWAVDKGWEA"
     gene            1002..>1084
                     /locus_tag="PLH31_20215"
     CDS             1002..>1084
                     /locus_tag="PLH31_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQR91551.1"
                     /translation="MVIVRPSTDADLPAITAIYAWNVLNGLG"
BASE COUNT          200 a          361 c          345 g          178 t
ORIGIN      
        1 gtcggcttcg acgaggccgc ctatgcgctc agcctcgagg gcggctacga gtacgacacc
       61 accacggttc gcttcgtcta caactcgatg accacgcccc ggcagtggtt cgactacgac
      121 atgaccagcc gccagcggac cctgcgcaag acccaggaga tcccgtcagg ccacgatccg
      181 gccaggtacg agacccgccg cctgaacgcc aaggcgtcgg acggtacaga ggtgccgatc
      241 ttcgtgctga tgaagaaggg gacccggctg gatgggtcgg ctccgctgct gctgtacggc
      301 tatggcagct atggcatctc gatggaggcc agcttctcta tccgcaattt cagcctggtg
      361 gatcgcggct ggatctgggc cacggcctgc ccgcgcggcg gctcggaaaa gggctggagc
      421 tggttcctgg acggcaagaa ggacaagaag aagaacacct ttaccgactt catcgcctgc
      481 gccgagcacc tgcacgcggc cggctatggc aagccggagc gcaccgtggc ctatggtggc
      541 tcggccggcg gcctgctgat gggcgccatc accaacatgc ggcccgacct gttcgccggg
      601 atcatcgcgg cggtgccgtt cgtcgacgtg gtcaacacca tgagcgacac taccctgccc
      661 ctgaccccgc ccgaatggcc cgaatggggc aatccgctgg aggacgcggc ggcctatgac
      721 tacattgcca gctacagccc ctatgacaat atcgcggcca agccctatcc ggcgatcctg
      781 gccaccggcg gtctctccga cccgcgcgtc acctattggg aacctgaaaa gtgggtggcc
      841 aagctgcggc cggccaccac cagcggcaag ccggtgctgc tgaagatcaa catggaggcc
      901 ggacatggcg gggcctcggg ccggtttgat ttcctcaagg aaatcgcgct cgactacgcc
      961 ttcgcggtct gggccgtcga caagggctgg gaggcctagg cttggtcatc gtccgcccat
     1021 caaccgacgc cgaccttccg gcaattacgg cgatctatgc ctggaacgtc ctgaacggcc
     1081 tggg
//
LOCUS       DAOMEO010001254         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13606_length_1081_cov_2.457115, whole genome shotgun sequence.
ACCESSION   DAOMEO010001254 DAOMEO010000000
VERSION     DAOMEO010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13606_length_1081_cov_2.457115"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLH31_20220"
     CDS             complement(<1..320)
                     /locus_tag="PLH31_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR91552.1"
                     /translation="MGEQEFCIDVKTVREIRGWTPATPIPHAPSYLRGVINLRGAIMP
                     VIDLRNRLGLGFTVPETRHVIVVVECQDRLAGILVDAVSETFTVSRDHLQPAPDLGVE
                     ELRF"
     assembly_gap    323..399
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(571..690)
                     /gene="cydX"
                     /locus_tag="PLH31_20225"
     CDS             complement(571..690)
                     /gene="cydX"
                     /locus_tag="PLH31_20225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HQR91553.1"
                     /translation="MWYFAWMLGLGLAVAFGVLNGVWYEFNLSDDGDEGLSEP"
     gene            complement(704..>1081)
                     /locus_tag="PLH31_20230"
     CDS             complement(704..>1081)
                     /locus_tag="PLH31_20230"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQR91554.1"
                     /translation="AWLDNYARHSWMILAPVLGFVGTAVALLGLRMDRAALSFSGSTL
                     ATLGIISTVGLSMFPFILPSAIDPRSSLTAWNASSSHLTLFIMLIVTVIFLPLILIYT
                     AWVYKVLWGRSSTTALTTNPDLY"
BASE COUNT          204 a          308 c          333 g          159 t
ORIGIN      
        1 atgaagcgca gctcctcgac gcccagatca ggcgccggct gcagatgatc gcgcgacacg
       61 gtgaaggtct cggagacggc gtcgaccagg atgccagcca ggcggtcctg gcactcgacg
      121 accacgatca cgtggcgggt ctcgggaacg gtgaagccca ggcccaggcg gttgcgcagg
      181 tcgatgaccg gcatgatcgc gccgcgcaga ttgatgaccc cgcgcagata ggacggtgcg
      241 tgcgggatcg gcgtcgccgg agtccagccg cgaatttcgc ggacggtctt cacgtcgatg
      301 cagaactcct gttcgccgat gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcttggtcgg tcatatcgat
      421 taccccggga tcgaaggtgt caggcgtgtc tcgggttcgc gtattcgacg cgctccagat
      481 cacgcatcca caagacgcga ttggcccttt aacaacggtt aacgctgacg ccgaagccgc
      541 gcgccggatt gtcgcaatcg cgtgaacggc tcagggctca gacagacctt cgtcgccatc
      601 gtccgacagg ttgaattcgt accagacgcc gttcagcaca ccgaaggcga cggccaggcc
      661 caggcccagc atccacgcga aataccacat cgttgcgtct ccctcagtag aggtcggggt
      721 tggtggtcag ggccgtcgtg ctgctgcgtc cccacagcac cttatagacc caggcggtgt
      781 agatcaggat cagcggcagg aagatcaccg tgacaatcag catgatgaac agcgtcaggt
      841 ggctggacga ggcgttccag gccgtcaggc tggagcgcgg atcgatggcg ctgggcagga
      901 tgaacgggaa catcgaaagg ccaacggtcg agatgatgcc cagggtcgcc agggtcgatc
      961 cggagaacga cagcgccgcc ctgtccatcc gcaggcccag cagggcgaca gcggttccga
     1021 caaagcccag cacaggggcc aggatcatcc agctgtggcg cgcatagttg tccagccagg
     1081 c
//
LOCUS       DAOMEO010001255         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13610_length_1081_cov_2.017544, whole genome shotgun sequence.
ACCESSION   DAOMEO010001255 DAOMEO010000000
VERSION     DAOMEO010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_1081_cov_2.017544"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLH31_20235"
     CDS             <1..205
                     /locus_tag="PLH31_20235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HQR91555.1"
                     /translation="TGRDALFLLDGAEPGDLAGFSRCILLFDGRDESALAQARGRWAS
                     FKKAGHPVSYWRQGAERGWEKQA"
     gene            202..540
                     /locus_tag="PLH31_20240"
     CDS             202..540
                     /locus_tag="PLH31_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor I78"
                     /protein_id="HQR91556.1"
                     /translation="MKTLMVVGAALAAAVLAVSCSSTPAPAPMPAPETPVAPPRAAIA
                     PPQPKDQCGMVEARAYVGKNRTELPAAVDPTRWRVACTTCPVTMDFRPDRLNILFDAD
                     TGTVKEVKCG"
     assembly_gap    704..713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <714..>1081
                     /locus_tag="PLH31_20245"
     CDS             <714..>1081
                     /locus_tag="PLH31_20245"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HQR91557.1"
                     /translation="GLVAGAHAGDSVLINFVLTPSNILQTWLCAWCTRRFLGPEIDLG
                     RPKHMIAFVGLAGFAVPVVVGTLASLYHTATRGGDVWANATIWVLGDILGVLIVTPCL
                     LVLVKAKTYLAERGLTREGL"
BASE COUNT          167 a          351 c          354 g          199 t
ORIGIN      
        1 gacaggcagg gacgcgctgt ttctgctgga tggcgccgaa cccggtgatc tggcgggctt
       61 ctcacgttgc atcctgctgt tcgacggccg cgatgagtca gcgctggccc aggcccgcgg
      121 gcggtgggcg agtttcaaga aagccgggca tccggtgtca tactggcgtc aaggcgccga
      181 gcgcggatgg gagaagcagg catgaagacg ctgatggtcg tcggggcggc gctggctgcg
      241 gctgttcttg cggtgtcctg ttccagcact ccggccccgg cgcccatgcc agcacccgag
      301 acgccggtgg cgccgccgcg cgccgcaatc gccccgcccc agccgaaaga tcagtgcggc
      361 atggtcgagg cccgggccta tgtcggcaag aaccgcaccg aactgcccgc cgccgtcgac
      421 cccacccgct ggcgcgtggc ctgcacgacc tgtcctgtga ctatggactt ccgaccggat
      481 cgtctgaaca tactgttcga cgccgacacg ggcacggtga aagaggtcaa gtgcggctag
      541 acaggcaaga catcggaaaa acgatgctcg tctcactgat tgcgggactg ggctttgccg
      601 tcttggccta tctctgcgtc gagattcccc gcgcctacaa ccaggccacc ccgatctggc
      661 tgtcgaacgg ctttgtggtg gcgtgcctgc tggccgtcga cacnnnnnnn nnncggattg
      721 gtggccggcg cccatgcggg cgactcggtt ctgatcaatt tcgttctgac ccccagcaac
      781 atccttcaga cctggctatg cgcgtggtgc acgcgccgtt tcctggggcc cgagatcgat
      841 ctgggccgtc ccaagcacat gatcgccttt gtggggcttg caggcttcgc cgtccccgtc
      901 gtcgtgggaa ccctcgcttc gctctatcat accgctaccc ggggcggaga cgtctgggcc
      961 aacgccacca tctgggtgct gggagacatt ctgggcgtcc tgatcgtgac gccgtgcctg
     1021 ctggtgctgg taaaggccaa gacctacctg gccgagcgcg gactgacacg cgagggtctg
     1081 c
//
LOCUS       DAOMEO010001256         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13611_length_1081_cov_2.014620, whole genome shotgun sequence.
ACCESSION   DAOMEO010001256 DAOMEO010000000
VERSION     DAOMEO010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13611_length_1081_cov_2.014620"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..363)
                     /locus_tag="PLH31_20250"
     CDS             complement(73..363)
                     /locus_tag="PLH31_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91558.1"
                     /translation="MPTETDIFEQLRQELRVERYGYSLRQALASVGVEIDEGALYPML
                     RRLEGQGLLSSEWREEDKRKKRFYRLSSEGEAVLARLADEWRAINDTLQPLL"
     gene            505..1077
                     /locus_tag="PLH31_20255"
     CDS             505..1077
                     /locus_tag="PLH31_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91559.1"
                     /translation="MRRRALALLLTAGLALASLPAAARAPLDIAALRMATAGPWEGKL
                     EYLDYSANRWFGIPMTVLIEDQGDGVTLIRKAQFDDGPRVGIVRITTVELFDPATGKE
                     TSASFRKGRETSLDTTTLRLPAPAADATHWTIVAETTGKDDNRPARLRQTLVRDGDVM
                     TSLKEVDFLDDDGEAWISRNRTTLKRVVGG"
BASE COUNT          176 a          367 c          356 g          182 t
ORIGIN      
        1 gctgggcggc gggcaggagg gcggcgacgg cgcccagata gcggtcgagc atatccatga
       61 tccgtcctct cctcaaagca ggggttgcag ggtgtcgttg atggcgcgcc attcgtcggc
      121 caggcgggcc agcacggcct cgccctcgct cgacaggcgg tagaaacgct tcttgcgctt
      181 gtcttcctcg cgccactcgc tggacagcag gccctgccct tccagccggc gcagcatggg
      241 atagagcgcg ccttcgtcga tctcgacgcc caccgaagcc agcgcctgac gcaggctgta
      301 gccgtagcgc tcgacccgaa gctcctggcg gagctgttca aagatgtcgg tttccgttgg
      361 catggtgtgc gccgtatcgt gtgcgccaca tggtgtgtga catacactgt gtgtcacaca
      421 gtgaattggg agtcaaacgc ctcgcatccc gatgttcgcc aagggcgtat ctttaggtga
      481 agctcgcccc cggagatccg attcatgcgc cgccgcgccc tcgccctttt gctgaccgcc
      541 ggcctggcct tggccagcct gcccgccgcc gcccgggccc cgctggacat cgccgccctg
      601 cgcatggcca cggcgggccc gtgggagggc aagctggaat atctcgacta ttcggccaac
      661 cgctggtttg gcatcccgat gacggtgctg atcgaggacc agggcgacgg cgtcaccctg
      721 atccgcaagg cccagttcga cgatggcccg cgcgtgggca tcgtgcgtat cactacggtc
      781 gagctgtttg atccggccac cggcaaggaa acctcggcca gcttccgcaa gggccgcgag
      841 acaagcctgg acaccaccac cctgcgcctg cccgctccgg ccgccgacgc gacccattgg
      901 acgatcgtcg ccgagaccac cggcaaggac gacaaccgcc ccgcccgcct gcgccagact
      961 ctcgtccgcg acggcgacgt gatgacgagc ttgaaggagg tcgacttcct ggatgatgac
     1021 ggcgaggcgt ggatctcgcg gaacaggacg acgctgaagc gggtggttgg gggctgagcc
     1081 g
//
LOCUS       DAOMEO010001257         1081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13616_length_1081_cov_1.740741, whole genome shotgun sequence.
ACCESSION   DAOMEO010001257 DAOMEO010000000
VERSION     DAOMEO010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13616_length_1081_cov_1.740741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLH31_20260"
     CDS             complement(<1..1072)
                     /locus_tag="PLH31_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91560.1"
                     /translation="MLTRTKQAISGNESGGRYDAVGPDTGSGKRAYGKYQVMDFNVGP
                     WTQEALGRPMTPQEFLASPDAQEAVADFKLGQYIKQYGSPEAAARAWFAGPGGMNNTA
                     AKDVLGTTVGGYGQKFAQAYGAGATGPQPAQGSDMPPADASGTPAPPQAQPDSDWPSV
                     RDFVKSQYPGAKVLGFRDGSIAYDKQGRVRIKRADGQDDAVDVPKAKAPGAEKGLFGD
                     SLTGRALTALRTLTPDQQDYAAAYAILSKPQVISDGAGGQTIIQPMDLSMYPKPAYGA
                     GRGVQTPAGGSAPTPIPGGGTIQNIPGKGKPLDNSARDDLTKASGGVLELTELVKSFD
                     SSFGGYMLDSLGDADNFRKRNLP"
BASE COUNT          163 a          360 c          357 g          201 t
ORIGIN      
        1 ccgggaggtt gcgcttgcgg aagttgtcgg cgtcgcccag cgagtcgagc atatagccgc
       61 cgaaagacga atcgaacgac ttcaccaact cggtcaattc aagcacgccg ccgctggcct
      121 tggtcagatc gtcgcgagcc gagttgtcga gcggcttgcc cttgcctggg atgttctgaa
      181 tggttccgcc gccgggaatg ggggtcgggg ccgagccgcc cgcaggcgtc tggacgccgc
      241 gccccgcgcc ataggccggc ttcgggtaca tcgacagatc catcggctgg atgatcgtct
      301 ggccgcccgc gccgtcgctt ataacctgcg gcttggacag gatggcgtag gccgccgcgt
      361 agtcctgttg gtcgggagta agggtgcgca gcgcggtcag cgcgcggccc gtcagagagt
      421 cgccgaacaa gcccttctcc gcgccgggcg ccttggcctt cggcacatcg acggcgtcgt
      481 cctgtccatc cgctcgctta atgcgaacac gcccttgctt gtcgtacgcg atagaaccgt
      541 ccctgaagcc caagaccttc gcgccgggat attgggactt cacaaagtca cgcacggacg
      601 gccagtcact gtccggttgg gcctggggag gagcaggcgt gcccgaggcg tcggcaggcg
      661 gcatgtcgga gccttgggcg ggctgaggcc ctgtagcgcc cgcgccatag gcctgtgcga
      721 acttctgccc gtatccgcca accgtcgtgc ccagaacgtc cttggccgca gtgttgttca
      781 tcccgccggg gccagcaaac cacgcgcggg ccgcagcctc ggggctgccg tactgcttga
      841 tgtactggcc gagcttgaag tctgcgaccg cttcctgcgc atccgggctt gcaaggaact
      901 cctgcggcgt catcggtcgg cccaaggctt cctgcgtcca cgggccgacg ttgaagtcca
      961 tcacctgata cttgccgtag gcgcgtttcc cgctgccggt atccgggccg acggcgtcat
     1021 agcgaccgcc gctctcgttt ccagaaatgg cctgcttcgt gcgggtaagc acgtcgccct
     1081 g
//
LOCUS       DAOMEO010001258         1080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13640_length_1080_cov_1.674146, whole genome shotgun sequence.
ACCESSION   DAOMEO010001258 DAOMEO010000000
VERSION     DAOMEO010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13640_length_1080_cov_1.674146"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..>1080
                     /locus_tag="PLH31_20265"
     CDS             314..>1080
                     /locus_tag="PLH31_20265"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQR91561.1"
                     /translation="MARTTRDAKSPAQQPSSQTSSLSSPSSQTQSQSEAEPPPPLTYE
                     VLTSDDAKRDALQLVADSIAQQRQVASLAVIFHPACFVAVVVGLGAMFGALLEAGGGV
                     EAIANRLLARFGEKRALWALTAIGVVVGVPLFFDVALIILAPLLCALTLRSKRPIVWF
                     ALPTLAGLAVMHALLPPHPGPVAAAELLKADYGQIALWGFVCGLPAVLVAGPFYAWIA
                     YRKQGQEGYVLPPSMDTGMPRQATIGFGVALTAMLAPL"
BASE COUNT          173 a          367 c          328 g          212 t
ORIGIN      
        1 cgggcggaca cgcgatataa caagtcgact attccctgac gtgggccgac tgttccgggc
       61 tgtagcgcac gtgtgccacg cggatattgt cttttttttt ctattcactt ggggcctgtt
      121 tatctctcgg gtggacaaag tgcgaggctg cttgttttgg gtggcagctg aggctcgcat
      181 tttcatttgc cattttggga gcggagctcc aaactcacgt accagctcac tcaacaccgt
      241 cgagtgggtt gcaggcatgg cttcgtcaac ctcaacctca aacaccacaa ccacaaccac
      301 cttggtcgcg gccatggcgc gcaccacccg cgacgcaaag tcgccagccc agcagccaag
      361 ctcacaaaca tcatcattat catcaccatc ctcacaaaca caatcacagt cagaagcgga
      421 gccgccgcca ccgctcacgt acgaggtcct caccagcgac gacgccaagc gcgacgcgct
      481 gcagctcgtc gccgacagca tcgcgcagca gcggcaggtc gcgtccctgg ccgtcatctt
      541 ccacccggcg tgcttcgtcg ccgtcgtcgt cgggctgggc gcgatgttcg gggccctgct
      601 tgaggccggg ggcggggtgg aggcgatcgc caacaggctg ctggcgcggt ttggcgaaaa
      661 gcgcgcgctt tgggccctga ccgcgatcgg tgtggtggtg ggcgtgccgc tgttcttcga
      721 cgtggccctg atcatcctgg cgccgttgct gtgcgccctg accttgagat ccaaacgccc
      781 gatcgtctgg ttcgccctgc cgaccctggc gggcctggcg gtgatgcacg ccctgctgcc
      841 gccccacccc ggcccggtcg ccgccgccga attgctgaag gccgactacg gtcagatcgc
      901 cctctggggc tttgtctgcg gcctgccggc tgtgctggtc gccggaccgt tctatgcctg
      961 gatcgcctat cgcaaacagg gccaggaagg ctacgtcctg ccgcccagca tggacaccgg
     1021 catgccgcgt caggccacca tcggctttgg cgtggccctg accgcgatgc tggcgccgct
//
LOCUS       DAOMEO010001259         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13657_length_1079_cov_1.833984, whole genome shotgun sequence.
ACCESSION   DAOMEO010001259 DAOMEO010000000
VERSION     DAOMEO010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_1079_cov_1.833984"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PLH31_20270"
     CDS             <1..162
                     /locus_tag="PLH31_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR91562.1"
                     /translation="GAQGLAWRILEAYNRLQTARVFAALLTLALLGVALHALMELAER
                     RLLAIWRGR"
     gene            223..903
                     /locus_tag="PLH31_20275"
     CDS             223..903
                     /locus_tag="PLH31_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91563.1"
                     /translation="MRILVLIAALVCVAPAAHAEEARENRYGPRPSRPTTSLNANTGY
                     AGPILGWAGKQDAIVSRAPAPQAVNEPWRQPYVAPQARQPMAAAQAQAPMSEPWRNLG
                     ARPQAGTPAPQALPPMSEPWRNLGARPQASAPAQAAPATMAAYSPPPQAVTPSMARTY
                     SVGRQYGLQPDALPPLQPNGMVFIAPSEEAPRLKDDEPRHGSAEWLAAGARGDEDEDR
                     DNARDGDR"
     gene            903..>1079
                     /locus_tag="PLH31_20280"
     CDS             903..>1079
                     /locus_tag="PLH31_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91564.1"
                     /translation="MQSKLHDLIALADEPSSTKRRELLRGVTDLFFTGDNHDPVQMGL
                     FDDVMSQLASEMEEV"
BASE COUNT          175 a          409 c          346 g          149 t
ORIGIN      
        1 ggcgcccaag gcttggcctg gcggattctc gaggcctaca atcggctgca gaccgctcgg
       61 gtgttcgccg ccctgctgac cctggccctg ctgggcgtgg ccctgcacgc cctgatggag
      121 ctggccgaac gtcgactgct cgccatttgg cgcgggcgtt aggtccggat taagcggaac
      181 ggactatggc tggaggctcg gagtcctccg tcaggccacc ttatgcgtat cctcgttctc
      241 atcgccgccc tcgtttgcgt cgcgcccgcc gcgcacgcag aggaagcacg cgagaaccgc
      301 tacggcccac gacccagccg accgacgacc tccctgaatg ccaacacggg ctatgccggc
      361 ccgattctgg gctgggccgg caagcaggac gccatcgtgt cccgcgcccc ggcgccgcaa
      421 gccgtgaacg agccctggcg ccagccctat gtcgcgcctc aggcgcgtca gccgatggct
      481 gctgcgcagg cccaggcgcc gatgtccgaa ccctggcgca atctgggagc acggccgcag
      541 gccggaacgc cggcgccgca agctctgccg ccgatgtcgg agccctggcg caacctgggc
      601 gcccggccgc aggcgtccgc cccagcccag gccgcgcccg cgaccatggc ggcctattcg
      661 cctccgcccc aggccgtgac gcctagcatg gcccgcacct attcggtggg tcgccagtac
      721 ggcctgcaac ccgacgccct gccgccgctt caacccaacg gcatggtgtt catcgccccg
      781 tccgaagaag ccccacgcct gaaagacgac gaaccccgcc acggctcggc cgaatggctg
      841 gccgccggcg cgcgcggcga cgaggacgaa gatcgcgaca acgccaggga cggcgatcgc
      901 tgatgcagtc caagcttcac gacctgatcg ccctggccga cgagccgtcc agcaccaagc
      961 gtcgcgaact gctgcgaggc gtcaccgacc tgttcttcac cggcgacaac cacgacccgg
     1021 tccagatggg cctgttcgac gacgtcatga gccagctggc cagcgagatg gaagaggtc
//
LOCUS       DAOMEO010001260         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13683_length_1078_cov_1.793744, whole genome shotgun sequence.
ACCESSION   DAOMEO010001260 DAOMEO010000000
VERSION     DAOMEO010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13683_length_1078_cov_1.793744"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1021
                     /locus_tag="PLH31_20285"
     CDS             71..1021
                     /locus_tag="PLH31_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQR91565.1"
                     /translation="MAKTGDQDRDALIAQASQWLARLDAGRTTELELNAWRDADPRRA
                     ATFAEVASAWSRLDVLRSDEARPAPGINRRAWLTGGGATLAASLAAGVYLGRDVLLRD
                     RVATGVGERRTLALPDGSSVELNTDTEIFWRYDRQRRRLWLARGEAALTIARDTLRPF
                     ELVTAQALTRLAAGQFNARLRPAGLDLVVLAGRAVIETAYATAEAGVAGADDARRVLE
                     VSDSKIAVVDAPEDRVQNVQAWRRGEIVFEGQALSVAVEEYNRYLTRKLVIGDTRAAN
                     LKLGGRFLTGDPDSFLQALRTTFGLRIVEDGPSRILLKSR"
BASE COUNT          170 a          359 c          388 g          161 t
ORIGIN      
        1 aggggcttcg gctcttgacc aaggccctcg ccgcgggtga tgggcgcagt atcgagaaac
       61 gaccgagttc atggccaaga cgggcgatca agacagagac gcgctgatcg cccaggcttc
      121 gcaatggctg gcgcggctgg acgccggccg cacgacagag ctggaactga acgcctggcg
      181 tgacgccgat ccgcgccgcg cggcgacctt cgcggaggtg gccagcgcct ggagccggct
      241 ggatgtgctg cgaagtgatg aagcccggcc cgcgcccggg atcaaccgac gcgcctggct
      301 gacgggcggc ggcgcgactc ttgccgccag cctcgcggcc ggcgtctatc tggggcggga
      361 cgtcctgctg cgcgatcgcg tcgcgaccgg cgttggcgaa cgtcgcaccc tggccctgcc
      421 cgacggcagt tcggtcgagc tgaataccga caccgaaatc ttctggcgct atgaccggca
      481 aagacgccgg ctgtggctcg cgcgcgggga ggccgccctg acgatcgcca gggacacgct
      541 gcgccccttt gaactggtca cggctcaggc cctgacgcgt ctggcggcgg ggcagttcaa
      601 tgcgcggctg cgcccggcgg ggctggacct cgtggtcctg gccggacgcg cagtgatcga
      661 gaccgcctac gccacggccg aggcaggcgt ggccggggcc gatgatgcgc gccgggttct
      721 ggaggtcagc gacagcaaga tcgccgtcgt cgatgcgccg gaagacaggg tccagaacgt
      781 gcaggcctgg cggcggggcg agatcgtgtt cgagggacag gccctgtccg tcgcggtcga
      841 ggagtacaac cgctacctga cccgcaagct ggtcatcggc gacacccgcg cagccaatct
      901 gaagctgggc ggccggttcc tgaccggcga tcccgacagc ttcctgcagg cgctgcgcac
      961 gacgttcggt ctacggatcg tcgaggacgg accgtcgcga attcttctta aatctcgata
     1021 ggggtttttc ccgcgccccc cgtcttgccc tgcgagacgc cgatcacggc gatcaacg
//
LOCUS       DAOMEO010001261         1077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13700_length_1077_cov_1.920744, whole genome shotgun sequence.
ACCESSION   DAOMEO010001261 DAOMEO010000000
VERSION     DAOMEO010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13700_length_1077_cov_1.920744"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /gene="ccsA"
                     /locus_tag="PLH31_20290"
     CDS             <1..>1077
                     /gene="ccsA"
                     /locus_tag="PLH31_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQR91566.1"
                     /translation="ILALVMSVAQSGLSAVAGALRLPMLAGAGRGAAVATFGATGIAF
                     ACLIWAFVTSDFSVANVAANSHTDKPLLFKVAGAWGSHEGSMLLWCVVLTGYGAAMAL
                     FGSTLPPRLRAYAVAVQGALGVMFLAYTVHPPFLYAGYVGFSVVYSLAMAALIDGRID
                     AAWARWVRPWTLAAWSLLTIGITLGAFWAYYELGWGGWWFWDPVENASFMPWLIGAAL
                     LHSAIVMEKRGALPGWTAFLALAAFTFSMLGAFLVRSGVLTSVHAFAVDPTRGVLLLI
                     MMGIAAGAGFLLFALRAPALNSGGLFAPVSRESAIVLNNIVLSTATATVLLGTLYPLI
                     REAIDGEAVSVGPPFFNLTFTPLMV"
BASE COUNT          109 a          374 c          378 g          216 t
ORIGIN      
        1 atcctcgcgc tggtcatgtc ggtcgcgcag agcggcctgt cggcggtggc cggcgccttg
       61 cggctgccca tgctggctgg cgcgggacgc ggcgcggcgg tggcgacgtt cggcgcgacc
      121 ggcatcgcct tcgcctgcct gatctgggcc ttcgtcacct ctgacttctc ggtcgccaac
      181 gtcgcggcca attctcacac cgacaagccg ttgctgttca aggtggccgg cgcctggggc
      241 agccatgaag gctcgatgct gctgtggtgc gtggtgctga caggctacgg cgcggccatg
      301 gccctgttcg gttcgaccct gccgccgcgc ctgcgggcct atgccgtggc ggtgcagggc
      361 gcgctgggcg tgatgttcct ggcctatacg gtgcatccgc ccttcctcta cgccggctat
      421 gtcggctttt cggtggtcta cagcctggcg atggccgccc tgatcgacgg ccgcatcgac
      481 gcggcctggg cccgctgggt gcggccctgg actctggcgg cctggagcct gctgaccatc
      541 ggcatcacgc tcggcgcctt ctgggcctac tacgagctgg gctggggcgg ctggtggttc
      601 tgggacccgg tcgagaacgc cagcttcatg ccgtggctga tcggcgccgc tctgctgcac
      661 tcggccatcg tcatggaaaa gcgcggcgcc ctgccgggct ggacggcctt ccttgccctg
      721 gcggccttta ccttctcgat gctgggcgcc ttcctggtgc gctcgggcgt gctgacctcg
      781 gttcacgcct ttgcggtgga tcccacgcgc ggcgtgctgc tgctgatcat gatgggcatc
      841 gcggcaggcg cggggttcct gctgttcgcc ctgcgcgccc cggccctgaa ctccggcggc
      901 ctgttcgcgc cggtcagccg cgagagcgcc atcgtgctca acaacatcgt gctgtcgacc
      961 gccacggcga ccgtgctgct gggcacgctc tatccgctga tccgcgaggc catcgacggc
     1021 gaggcggtgt cggtggggcc gccgttcttc aacctgacct tcaccccgct gatggtg
//
LOCUS       DAOMEO010001262         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13809_length_1071_cov_2.348425, whole genome shotgun sequence.
ACCESSION   DAOMEO010001262 DAOMEO010000000
VERSION     DAOMEO010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13809_length_1071_cov_2.348425"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLH31_20295"
     CDS             complement(<1..772)
                     /locus_tag="PLH31_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HQR91567.1"
                     /translation="MAVSGEQPIIIKKVKKVVGGGHHGGAWKVAYADFVTAMMAFFLL
                     MWLLNSTSPEQKQGIADYFAPASISSTTSGSGGILGGTSLGDDGAKADGKLSVIQQMA
                     PEAPEQTDKEGQSSTTASLDSASEEALQDAMAKKQQDSFASAAQSLRQSLQEMPELAE
                     LSKQIMIDQTPEGLRIQLIDQEGRSMFKENSKEPNDRAKILLRAVAKIINQLPNRVTV
                     SGHTSANALGRKSDSDWALSATRADAARLVLRNAGVNDD"
     gene            887..>1071
                     /locus_tag="PLH31_20300"
     CDS             887..>1071
                     /locus_tag="PLH31_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQR91568.1"
                     /translation="MKTAFIGLGVMGYPMAGHLKAAGHEVSVFNRSPDKAQRWAAQHG
                     GAAFETIEEAVAGCELVA"
BASE COUNT          165 a          326 c          357 g          223 t
ORIGIN      
        1 ggtcgtcatt gacgcctgca ttgcgcagca ccaggcgcgc ggcgtcggct cgcgtggccg
       61 acagcgccca gtcactgtcg ctcttgcgac ccagggcgtt ggcgctggtg tgacccgaga
      121 cggtaacccg gtttggcagt tggttgatga tcttggccac cgcgcgcaac aggatcttgg
      181 cgcgatcatt gggctccttc gagttctcct tgaacatcga acggccttcc tgatcgatca
      241 gctgaatccg gagcccctcc ggggtctggt cgatcatgat ctgtttggaa agctcggcca
      301 actccggcat ttcctgtagc gactggcgaa gggactgggc cgccgaggcg aagctgtcct
      361 gctgcttctt ggccatggcg tcttgcagcg cctcttcgct ggctgagtcg aggctagcag
      421 tcgttgagct ctggccttcc ttgtcggtct gttcgggggc ctcgggcgcc atctgctgga
      481 tcaccgacag cttgccgtcg gccttggccc cgtcgtcgcc cagagaggtg ccgcccagga
      541 tgccgcccga accgctggtc gtcgacgaga tgctggcggg cgcgaaatag tccgcaatgc
      601 cctgcttttg ctcggggctg gtggaattca gcagccacat cagcaggaag aacgccatca
      661 tcgcggtcac gaagtcggca taggcgactt tccacgcgcc gccatggtgg ccgccgccga
      721 ccaccttctt gaccttcttg atgatgatcg gctgttcgcc gctgacggcc atcgtgctta
      781 agcctgctta accctgttcg ctggaagttg ccctgcggac gttaagatgg ggttgcgaag
      841 tgggcgcggg cgggcgaaat cgctggctcc ttgaggagtt ggcgacatga agacggcgtt
      901 tatcggcctg ggcgtgatgg gctacccgat ggcgggccac ctgaaggccg ctggtcacga
      961 ggtttccgtc ttcaatcgca gcccagacaa agcgcagcgc tgggcggcgc agcacggcgg
     1021 cgcagccttc gagacgattg aagaagctgt cgcgggctgc gagctggtcg c
//
LOCUS       DAOMEO010001263         1069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13843_length_1069_cov_2.235700, whole genome shotgun sequence.
ACCESSION   DAOMEO010001263 DAOMEO010000000
VERSION     DAOMEO010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_1069_cov_2.235700"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..>1069)
                     /locus_tag="PLH31_20305"
     CDS             complement(40..>1069)
                     /locus_tag="PLH31_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013480864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQR91569.1"
                     /translation="TDLWRATAPIDGAKYGVWPTGGAWLCKHLWDRYDYGRDTAYLAD
                     VYPLLKGAALFFLDALVVDPRSGRLVTNPSISPENDHGHGSSLVAGPTMDQAIVRDLF
                     HSCIASARILGQDTDLAAQFAAARDKLAPYRIGKDGQLQEWQDDWDADAPEQDHRHVS
                     HLYGLFPADQISLDTTPDLATAARKTLETRGDLSTGWAIAWRLNLWARLGRGDHAHGI
                     LTLLLGPERTYPNLFDAHPPFQIDGNFGGTSGMVEMILQSRNGAVHLLPALPSAWPTG
                     AMTGLKARGALDVDVWWKDGRLERARLTAVKAGRHRVVVGGQSLVVDMRQGETVELGL
                     QGGELRRV"
BASE COUNT          161 a          357 c          385 g          166 t
ORIGIN      
        1 cggctgacac cttctcccgc aaggggagaa gggtggtttc tacacccgcc tcagctcacc
       61 gccctgcagg cccagctcaa ccgtctcgcc ctgccgcatg tcgaccacca gcgactgccc
      121 gccgaccacc acgcggtgac ggccggcctt gaccgccgtc agccgggcgc gctccaggcg
      181 tccgtccttc caccacacat cgacatcaag agcgccgcgc gccttcaggc ccgtcatggc
      241 gccggtcggc caggccgagg ggagggccgg gagcaggtgg acggcgccgt tgcggctttg
      301 caggatcatt tcgaccatgc cgcttgtccc gccgaaattg ccgtcgatct ggaacggcgg
      361 atgggcgtcg aacaggttgg gataggtgcg ctcgggaccg agcagcaggg tcaggatgcc
      421 gtgcgcatga tcgccccggc ccagccgggc ccacaggttc agccgccagg cgatggccca
      481 gccggtcgac aggtcgccgc gggtctcaag ggtcttgcgc gcggcggtgg ccaggtcggg
      541 cgtggtgtcc aggctgatct ggtcggcggg aaacaggcca tacaggtgcg agacgtggcg
      601 gtgatcctgc tcgggggcgt cggcgtccca gtcgtcctgc cattcctgaa gctggccgtc
      661 cttgccgatc cggtagggcg cgagcttgtc gcgggcggcg gcgaactggg cggccaggtc
      721 ggtgtcctga cccaggatcc tggccgaggc gatgcagctg tggaacaggt cgcgcacgat
      781 cgcctggtcc atggtcggtc ccgcgaccag ggaggagccg tgcccgtgat cgttctccgg
      841 cgagattgac gggttggtga ccagcctgcc gctcctggga tccaccacca gggcgtcgag
      901 gaagaacagg gccgcgccct tcagcagcgg atagacgtcg gccagatagg cggtgtcgcg
      961 gccgtagtcg taacggtccc aaagatgctt gcacagccag gccccgcccg tgggccagac
     1021 gccatatttg gcgccgtcga tgggcgcggt ggcgcgccac aggtcggta
//
LOCUS       DAOMEO010001264         1068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13866_length_1068_cov_1.855874, whole genome shotgun sequence.
ACCESSION   DAOMEO010001264 DAOMEO010000000
VERSION     DAOMEO010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13866_length_1068_cov_1.855874"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLH31_20310"
     CDS             <1..664
                     /locus_tag="PLH31_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91570.1"
                     /translation="VAPWAADQTRRAIGPDRDMRLRKGEANRTDKALGRDPACPCPVG
                     VRMQKMIAALALAAMMSAGLANGALAATRSAKSAKAETPPDQRVWTIAKSEDTYALRF
                     ALPRAVDPVFAATCQPSAQLLQIAVEVPSDKKVRSGDGVPLSLSNGKRRLELAATAFQ
                     GAGDGLLVVEAAVTLDARVLELFRDGDTLTAKMPGSTQTFPLAGARSRLVDFERVCLL
                     KR"
     gene            788..>1068
                     /locus_tag="PLH31_20315"
     CDS             788..>1068
                     /locus_tag="PLH31_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91571.1"
                     /translation="MFRTLFAAGLLAATCLPAAAQEKLVWATTASDDGATLIFGVPET
                     DNAMISFTCNKGNPLVLVSSMIGSKGLKVDDAAKVILIAGKVKKEFAGKA"
BASE COUNT          188 a          338 c          343 g          199 t
ORIGIN      
        1 ggtcgcgcct tgggccgccg accaaacccg tcgcgcgatc gggccggacc gtgatatgcg
       61 attgcgcaag ggcgaggcaa acagaaccga taaggcgttg ggacgcgatc ctgcttgtcc
      121 ttgcccggtg ggagtgcgta tgcagaagat gatcgccgcc ctggctcttg cggccatgat
      181 gtctgcgggg ctcgccaatg gcgccctggc cgcgaccagg tccgccaaat ccgccaaggc
      241 cgagacgccg ccggatcagc gcgtgtggac cattgccaag tccgaagaca cctatgccct
      301 gcgtttcgcg ctgccgcggg ccgtcgatcc ggtgttcgcc gcgacgtgcc agcccagcgc
      361 acagcttctt cagatcgccg tcgaggtgcc gagcgacaag aaggtgcggt cgggcgacgg
      421 cgtgccgctg tccctgtcca acggcaagcg ccgcctggaa ctggccgcga cggctttcca
      481 gggcgcgggc gatggcctcc tggtggtgga agcagcggtg accctcgacg cgcgggtgct
      541 ggaactgttc cgcgacggcg acacgctgac cgcaaagatg ccgggttcca cccaaacctt
      601 tccccttgcc ggagcccgct cccgtcttgt cgacttcgag cgcgtctgcc tgctcaagcg
      661 ctgaacaaac cgcgattaat cttgagcgga gcgcggtttg actggtcatc ttaacctaga
      721 attaaggacg ctcttttcgt cttcggcgcg atgcgtatga tcctgcccaa atgatcgggg
      781 agatcgcatg ttccgcaccc tttttgctgc cggcttgctg gctgccacct gcctgcctgc
      841 tgccgctcag gaaaagctcg tctgggcaac cacggcgagc gacgatggcg caacgctgat
      901 ctttggcgtg ccggaaaccg acaacgccat gatttccttc acctgcaata agggcaatcc
      961 cctggtgctg gtcagttcga tgatcgggtc caaaggcctg aaggtggacg acgcggccaa
     1021 ggtgatcttg attgccggca aggtgaagaa ggaattcgcc ggcaaggc
//
LOCUS       DAOMEO010001265         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13899_length_1066_cov_2.580613, whole genome shotgun sequence.
ACCESSION   DAOMEO010001265 DAOMEO010000000
VERSION     DAOMEO010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13899_length_1066_cov_2.580613"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..>1066
                     /locus_tag="PLH31_20320"
     CDS             72..>1066
                     /locus_tag="PLH31_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQR91572.1"
                     /translation="MARPTKMSLRITLVFSVGVLLLVMGLGITLSGWVVIQADAQRQA
                     KSQARALLDSYGQSIGKDVGLSIKNAQTAAATVESLVADPALVNRDQIGGMIRHLVEA
                     NPGFVGMTPVFDANALDGRDAEFVSHPMSDANGRFVPYYYWTPEKTIALDKLVMTTEA
                     GIDSWYTKPIAENRDLITPPYLYPVGGKKILMTSAVAVIRRDASPIGVVTTDIALTDL
                     VARAEALRPFGEGRVSIVAGSVWVANADPALLGAPVADKPLMALLEQSNAGRYAQGEI
                     KGPKGMMLRAAAPVTLPGVADTWHIVVEAPMSAVMAGANKALTIMVLAGLAFMAAA"
BASE COUNT          179 a          358 c          349 g          180 t
ORIGIN      
        1 atggttacgc ttaggaacac cttcagagat tttcgcgaca ttgatcgtga ttaatccgca
       61 ggagatccgg cgtggcccgt ccgacgaaaa tgtcacttcg catcaccttg gtcttctcgg
      121 tgggcgtgct gctgctggtc atggggctgg gcatcacgct ctcgggctgg gtggtgatcc
      181 aggccgacgc ccagcgtcag gcaaagagcc aggcgcgcgc cctgctcgac agctatggcc
      241 agagcatcgg caaggacgtg ggcctgtcga tcaagaacgc ccagaccgcc gccgcgaccg
      301 tcgagtcgct ggtcgccgat ccggccctgg tgaaccgcga ccagatcggc ggcatgatcc
      361 gtcatctggt cgaggccaat cccggctttg tgggcatgac cccggtgttc gacgccaacg
      421 ccctggacgg ccgcgacgcc gagttcgtca gccatccgat gtcggacgcc aacggccgct
      481 tcgtgcccta ctattactgg accccggaaa agaccattgc gctggacaag ctggtgatga
      541 ccaccgaggc cggcatcgac tcctggtaca ccaagcccat cgccgaaaac cgcgatctga
      601 tcaccccgcc ctatctctat ccggtcggcg gcaagaagat cctgatgacc tcggccgtgg
      661 cggtcatccg gcgtgacgcc agcccgatcg gcgtggtgac caccgatatc gccctcaccg
      721 acctggtggc gcgggccgaa gccttgcgcc cgtttggcga agggcgggtg tcgatcgtcg
      781 cgggctcggt ctgggtagcc aacgccgatc cggccctgct gggggcgccc gtggccgaca
      841 agcccctgat ggcgctgctg gaacaatcga acgccggccg ctacgctcag ggcgagatca
      901 agggtcccaa gggaatgatg ctgcgcgccg ccgcgccggt caccctgccg ggcgtggccg
      961 acacctggca catcgtggtc gaggcgccga tgtcggcggt gatggccggc gccaacaagg
     1021 ccctgacgat catggtcctg gccgggctgg ccttcatggc cgccgc
//
LOCUS       DAOMEO010001266         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13912_length_1066_cov_1.764590, whole genome shotgun sequence.
ACCESSION   DAOMEO010001266 DAOMEO010000000
VERSION     DAOMEO010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13912_length_1066_cov_1.764590"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..852)
                     /locus_tag="PLH31_20325"
     CDS             complement(34..852)
                     /locus_tag="PLH31_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadD"
                     /protein_id="HQR91573.1"
                     /translation="MCRKRALLATVLAPALLMLGAPAQAGAAKTPPAAVDPAASRKAT
                     PAQRAEMRRADPLARAAFWGGEFETDGRDAEAGVALAQALRALGRYDDAAETAGKVLM
                     VQPDNIDALLESARAHIARGQGFYAIEPARRAAALAPRDWRPQSLLGVAYEQAQRDDE
                     ASIAHRQAVALGPNEAVPNANYAMHLASQGDLAGAEAQLRRAAVMPASSIQIRQNLAL
                     VIGLQGRIAEAEKLARQDLPPESVANNLAWLRAATSQPRPAGQSRSWEAMKSGG"
BASE COUNT          133 a          358 c          413 g          162 t
ORIGIN      
        1 gcctgttgcg gcgtacttcc ggggtcaaag gcctcagccg ccggacttca tcgcctccca
       61 cgaccgggat tgacctgcgg gcctgggctg gctggtggcg gcgcgcagcc aggccaggtt
      121 gttggctacc gattccggag gcaggtcctg acgggccagc ttttcggcct cggcgatgcg
      181 accctgcagg ccgatcacca gggccaggtt ctgacggatc tggatgcttg aggcgggcat
      241 gaccgccgcg cggcgtagct gggcctcggc gccggccagg tcgccctggc tggccagatg
      301 catggcgtag ttggcgtttg gcacggcctc gttggggccc agggccacgg cctggcggtg
      361 agcgatgctg gcctcgtcgt cgcgctgggc ctgctcatag gccacgccca gcagcgattg
      421 cggccgccag tcgcgggggg ccagggcggc ggcgcgccgg gccggctcga tggcgtagaa
      481 gccctggccg cgggcgatat gggcgcgggc cgattccagc agggcgtcga tattgtcggg
      541 ctggaccatc aacaccttgc cggcggtctc tgcggcgtcg tcatagcggc ccagcgcccg
      601 cagggcctgg gccagggcga cgcctgcctc ggcgtcacgg ccgtcggtct cgaactcgcc
      661 gccccagaag gcggcgcggg ccaggggatc ggcgcggcgc atctcggccc gctgggccgg
      721 cgtggccttg cgcgaggcgg cagggtcgac ggcggcgggc ggggtcttgg ctgcgccggc
      781 ctgggcgggc gcgcccagca tcagcagggc cggggcgaga acggttgcga ggagcgcgcg
      841 cttgcgacac atggaagaca agtctccgag agttggccgc atcctcgccg cgcggctctc
      901 aaggaatcgt taagcataaa tcccaagggc cgcgacatgt tggacagctc cccgatcctg
      961 gcttccgcaa cggagggcgg cgcgccgatc catccgctgt tcgaggccga gcttgagacg
     1021 ttcctggccg cgcgccgccc ttcggtccgg gtgctggccg cccagg
//
LOCUS       DAOMEO010001267         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13920_length_1065_cov_2.291089, whole genome shotgun sequence.
ACCESSION   DAOMEO010001267 DAOMEO010000000
VERSION     DAOMEO010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_1065_cov_2.291089"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLH31_20330"
     CDS             complement(<1..242)
                     /locus_tag="PLH31_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQR91574.1"
                     /translation="MISHRERHLIERVGWLRAAVLGANDGIVSTASLIVGVAAASQGR
                     GEILVVGVAGLVAGAMSMASYLHLVPCNLNLKTRGF"
     gene            484..>1065
                     /locus_tag="PLH31_20335"
     CDS             484..>1065
                     /locus_tag="PLH31_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQR91575.1"
                     /translation="MEQLNILLVGSGGREHALAWKIAQSPLCGRLVAAPGNPGIAALA
                     EVRAIKVTDVEGLVALAQEIAADLVVVGPESALEVGLADRLAALNIPCFGGSQRAARL
                     ETSKAFTKDFCARHDLPTAAYGVFTDAQGASAFLDTLQAPFVIKADGLAAGKGVVIAA
                     DRAQADAAVLDMLGGRFGTAGARVVIEEFMHGEE"
BASE COUNT          163 a          353 c          375 g          174 t
ORIGIN      
        1 ctgaatcccc ttgttttaag gttcaagtta caaggtacaa ggtgcaagta actggccatc
       61 gacatggcgc cggcgaccag acccgccacc ccgaccacga ggatctcgcc ccgcccctgc
      121 gaggcggcgg cgacgccgac gatcaggctg gcggtcgaga cgatcccgtc attggcgccc
      181 aggacggcgg cccgcagcca gccgacgcgt tcgatcagat ggcgttcgcg gtggctgatc
      241 atgaggttgt atcctggccg gatcgatcgg gctgcaccca cagcatctgc ggcacggtgc
      301 ggtcgcgatc caggatcatg tgcctggtgt ttgcaagggt catgcaggcg tctcccggag
      361 gcctcaagcc aagcccttta ggtcatgctg cgccttgacc tgtgtcaaac gtccgtcaac
      421 ggggcagggc gcgagggggt aaaaccttga ccccgccgcc ggcacgcgac acaccccggc
      481 ctcatggaac agctgaacat cctcctcgtc gggtccggcg ggcgcgagca cgccctggcc
      541 tggaagatcg cgcagtcgcc gctgtgcgga cgcctcgtgg ccgccccggg caatccgggg
      601 atcgcggcgc tggccgaggt gcgcgcgatc aaggtcaccg atgtcgaggg gctggtcgcc
      661 ctggcccagg agatcgccgc ggatctggtc gtggtgggtc cggaatcggc gcttgaggtt
      721 gggctggccg accgtctcgc cgcgctgaat atcccctgtt tcggcggcag ccagcgcgcc
      781 gcgcggctgg agacctccaa ggccttcacc aaggacttct gcgcccgcca tgacctgccg
      841 acggcggcct atggcgtgtt taccgacgcg cagggcgcgt cggctttcct cgacaccctg
      901 caagccccgt tcgtgatcaa ggccgacggt ctggcggccg gcaagggcgt ggtgatcgcc
      961 gctgaccggg cccaggccga cgccgccgtg ctcgacatgc tgggcgggcg gtttggcacg
     1021 gccggggcgc gggtggtgat cgaggagttc atgcatggcg aggag
//
LOCUS       DAOMEO010001268         1065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13924_length_1065_cov_1.855446, whole genome shotgun sequence.
ACCESSION   DAOMEO010001268 DAOMEO010000000
VERSION     DAOMEO010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13924_length_1065_cov_1.855446"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLH31_20340"
     CDS             complement(<1..379)
                     /locus_tag="PLH31_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR91576.1"
                     /translation="MTSTLKVPPVRRSQSDRRQQSESELLRAAAELIVERGMAAATFE
                     NIGARAGYSRGLATQKFGSKQGLIEALIARLQSRLQQLLEAGQLDTLDGLDAILGFVD
                     IYLRNLADDGEMRAYFVLMAGAVA"
     gene            464..>1065
                     /locus_tag="PLH31_20345"
     CDS             464..>1065
                     /locus_tag="PLH31_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR91577.1"
                     /translation="MTIPVDIANTIVDPKAYADGDRIDQAFAQLRRESPLEVAQPDGF
                     DPFWVVTRHADILEVERQNELFHNGDRATIVTNIEADKKVREMMGGSPHLVRSLVQMD
                     NPDHFAYRRVTQGALLPQNLRTLETRIREIARGFIDRMAAQGDRCDFAREVAFLYPLH
                     VIMEVLGVPEADEPRMLKLTQELFGSADPDLNRDGKVATDV"
BASE COUNT          196 a          371 c          326 g          172 t
ORIGIN      
        1 cggccacggc cccggccatc agcacgaaat aggcgcgcat ctcgccgtca tcggccaggt
       61 tgcgcagata gatgtcaacg aagcccagga tcgcgtccag cccgtcgagc gtgtccagct
      121 ggccggcctc cagcagctgt tgcagccgcg actgcagacg cgcgatcagc gcctcgatca
      181 gcccctgctt ggacccgaac ttctgggtcg ccagcccccg gctgtagccc gcccgcgcgc
      241 cgatgttctc gaaggtggcg gccgccatgc cgcgctcaac gatcagctcg gccgccgctc
      301 gcaacagttc gctctctgac tgctgacgcc ggtcggactg gctgcggcga acgggtggaa
      361 ccttaagggt cgatgtcatc ttgcggaggc tacttatttg ttgaacaact agcaaatatc
      421 aaaggccgcc gcagagccgc ccggaagcgc caggagcccg tgcatgacca tccctgtgga
      481 tatcgcaaac accatcgtcg atccaaaggc ctatgccgac ggcgaccgca tcgaccaggc
      541 gtttgcgcag ctgcggcgcg agagcccgct ggaggtggcc cagcccgacg gctttgaccc
      601 cttctgggtg gtcacgcgcc atgccgacat cctggaggtc gagcgccaga acgagctgtt
      661 ccataacggt gaccgcgcca cgatcgtcac caatatcgag gccgacaaaa aggtccgcga
      721 gatgatgggc ggttcgcccc acctggtgcg ctcgctggtc cagatggata accccgacca
      781 ctttgcctat cgccgcgtaa cccagggcgc gctgctgccg cagaacctgc gaacccttga
      841 gacgcggatt cgcgagatcg cccgcggctt catcgaccgc atggccgccc agggcgaccg
      901 ctgcgacttc gcccgcgaag tagccttcct ttatccgcta cacgtgatca tggaggttct
      961 gggcgtgccc gaggccgacg agccgcgcat gctgaagctg acccaggaac tgttcggcag
     1021 cgccgacccc gatctgaacc gcgacggcaa ggtcgccacg gacgt
//
LOCUS       DAOMEO010001269         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13941_length_1064_cov_2.848365, whole genome shotgun sequence.
ACCESSION   DAOMEO010001269 DAOMEO010000000
VERSION     DAOMEO010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_1064_cov_2.848365"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..948
                     /locus_tag="PLH31_20350"
     CDS             19..948
                     /locus_tag="PLH31_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HQR91578.1"
                     /translation="MLISHEMLMPLQPLLESAYTVHRLWDYPDHLAFLDGPGRGVRAI
                     VHAGEMSLARDMLSEMPRLGLIACVSVGYDGVDVPWCKAHGISVTHSTGLNASDVADH
                     AVGLMLAAWRGIVEGDHRLRAGRWTHAERMSPRHGLRGRKVGIVGLGHIGEAVARRVE
                     AFEMKVAWWAPRAKETDWPRAESLLALARDSDVLMVCARPDASNRHMISRAVIEALGP
                     QGLLVNVARGSLIDEDALIEALKAGTLGMAALDVFEQEPAKPERWADVPHTVLTPHTA
                     GATLDSIPAMVSLTMENLRCYFNGEPLASPVGD"
     gene            952..>1064
                     /locus_tag="PLH31_20355"
     CDS             952..>1064
                     /locus_tag="PLH31_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR91579.1"
                     /translation="MARVLGLGGVFFKAADPAAVREWYARVLGFTVHDWGGA"
BASE COUNT          154 a          353 c          383 g          174 t
ORIGIN      
        1 cccgtcgaaa agcgccacat cctgatctcg cacgagatgc tgatgccgct gcagcccttg
       61 ctggagagcg cctatacggt ccaccggctt tgggactatc ccgaccatct ggcgttcctt
      121 gacgggccgg ggcgtggcgt gcgggccatt gtccatgccg gcgagatgag cctggcgcgc
      181 gacatgctgt cggaaatgcc caggcttggc ctgatcgcct gtgtcagcgt cggctatgac
      241 ggcgtcgacg tgccatggtg caaggcgcat ggcatctcgg tgacccactc cacggggctg
      301 aacgccagcg acgtggccga ccatgcggtg ggcctgatgc tggcggcctg gcgcggcatc
      361 gtcgagggcg accatcgtct gcgggccggg cgctggaccc acgccgaacg catgtcgccc
      421 cgccacggcc tgcgcggccg caaggtgggt atcgtgggcc ttggccatat cggcgaggcc
      481 gtggcgcgcc gcgttgaggc cttcgagatg aaggtcgcct ggtgggcccc gcgcgccaag
      541 gaaaccgact ggccccgcgc cgaaagcctg ctggccctgg cccgcgacag cgacgtgctg
      601 atggtttgcg cccggcccga cgcctccaac cggcacatga tctccagggc tgtgatcgag
      661 gcgctgggac cgcagggcct gctggtcaat gtggcgcgcg gctcgctgat cgacgaggac
      721 gccctgatcg aggccctgaa ggccggtacc ctgggcatgg ccgcgctgga cgtcttcgag
      781 caggagcccg ccaagccgga gcgctgggcc gatgttcccc acaccgtcct gacgccgcat
      841 accgccgggg cgacgctcga cagcattccg gccatggtca gtctgacgat ggaaaacctg
      901 cgctgctatt tcaacggcga gccgctggcc tcgccggtcg gagactgaac catggccagg
      961 gtgctgggcc tgggtggagt gttcttcaag gccgccgacc cggccgccgt gcgtgagtgg
     1021 tatgcgcggg tgctgggctt tacggtccac gactggggcg gggc
//
LOCUS       DAOMEO010001270         1064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13942_length_1064_cov_2.690783, whole genome shotgun sequence.
ACCESSION   DAOMEO010001270 DAOMEO010000000
VERSION     DAOMEO010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13942_length_1064_cov_2.690783"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..412)
                     /locus_tag="PLH31_20360"
     CDS             complement(248..412)
                     /locus_tag="PLH31_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HQR91580.1"
                     /translation="MKVLLTRFVKEERGSTALEYGLIAAGLSIAIVTVLAQIGLTLAR
                     LKAATAIAGN"
     gene            complement(636..>1064)
                     /locus_tag="PLH31_20365"
     CDS             complement(636..>1064)
                     /locus_tag="PLH31_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91581.1"
                     /translation="VAYLLSVGVVAGLAYAAIAFGARAKARAARGWPIVNGRLVRPAG
                     RSSWGELGGGLRYSYDCAGQSYEGGRVRFLDWPSVLWGDARELRARLLRDEVEVRVNP
                     RLADDAVLDVTPPPARFGWIGLGIAAGSLLLMGVLRMLAH"
BASE COUNT          200 a          360 c          376 g          128 t
ORIGIN      
        1 gcagggcgaa gcccaccagt agcgcgatca cggcggcgtt gggaatgatg cgccggagca
       61 ggtcggtcgc gatcacgagc gccagcgcga ggggaaacag ccccaggatc agcgcgtctg
      121 ccaccatccg taccatgcgc gtccggcctc gaaccgtcag ggggtcacgc gcggcagacg
      181 ccggatcgcg tgagaaaaag agcaaaggac cggccccggt tcgccggacc ggagttcgga
      241 gacagggtca gtttccggcg atggcggtgg cggccttcag gcgcgcgagg gtcagcccga
      301 tctgcgccag aacggtgacg atggcgatgg acaggcccgc cgcgatcagc ccgtattcga
      361 gcgccgtgga accgcgctct tccttcacga agcgggtcaa cagaactttc attcgccctt
      421 ccctcggccg cagccttgtc cgggcatgcg gcagcccgga tgacagcagg ctagggcgac
      481 gtcccttgcg cagaggtaaa gatcggggag agcggggcgg cgaccatgag tcagcgtgcc
      541 caaagtgtta acgcggcggc gtccggcggc ataagacggt cagcggtgtg gccgagcgag
      601 ccctatctac ggccttgagc atgcagccga aatggtcagt gagccagcat ccggagcacg
      661 cccatcagga gcaggctgcc ggccgcaatg ccgaggccga tccagccgaa gcgggcgggc
      721 ggcggggtca cgtccagcac ggcgtcgtcc gcaaggcggg gattgacgcg cacctccacc
      781 tcgtcccgca gcaggcgggc acgcagttcg cgcgcatctc cccacagcac cgatggccag
      841 tcgagaaagc ggacgcggcc gccttcatag gactggccgg cacaatcata gctgtagcgc
      901 aggccgcccc ccagttcccc ccaggaggac cggcccgccg ggcgcacgag acgcccgttc
      961 acaatgggcc agccccgcgc ggcccgcgcc ttggcgcgcg cgccgaaggc gatggcggcg
     1021 taggcgaggc ctgcgaccac gcccacggac aaaagatagg ccac
//
LOCUS       DAOMEO010001271         1063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_13970_length_1063_cov_1.966270, whole genome shotgun sequence.
ACCESSION   DAOMEO010001271 DAOMEO010000000
VERSION     DAOMEO010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13970_length_1063_cov_1.966270"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLH31_20370"
     CDS             <1..250
                     /locus_tag="PLH31_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91582.1"
                     /translation="DAIVKAAHRTPFTASDTYSEVDAVKLKAVVMREAATLVGKGKVV
                     SVEITKQIARKQIPHPSGPKRAAAAASGGHAAAAAAHH"
     gene            505..>1063
                     /locus_tag="PLH31_20375"
     CDS             505..>1063
                     /locus_tag="PLH31_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQR91583.1"
                     /translation="MSSWLILAIAAGLLAVLYGAVQTASLMRASPGNAKMQEIAAAIQ
                     EGAQAYLNRQYTTIALVGVVVIALLAFFFKSWEQPVGFAAGFAGMLISVRANVRTAQA
                     SSESLAKGLSMAFTSGAVTGMLVAGFALIGVAGYFALLTGPGGYAGTDRVVVDSLVSL
                     GFGASLISIFARLGGGIFTKGADVGG"
BASE COUNT          151 a          360 c          352 g          200 t
ORIGIN      
        1 tgacgccatc gtcaaggcgg cccatcgtac gcctttcacg gcatccgaca cctatagcga
       61 ggttgacgcc gtcaagctca aggccgttgt gatgcgcgag gccgccaccc ttgtgggcaa
      121 gggcaaggtc gtctcggtgg agatcaccaa gcagatcgcc cgcaagcaga tcccgcatcc
      181 gtccggcccg aagcgcgccg cggctgccgc gtccggcggc catgccgccg ccgctgccgc
      241 gcaccactag gctcgcttcg aacaatcgtt tcatggcgcc cggcggcctg cacgcccacg
      301 ggttgcgcac ccctgtccca tttggcactg tccacgcctt ggcgcgtggt gcgcgccaat
      361 tcagggagca tcgagggggc atgaggccat aaggcccgcc cgtcgatccg gacgatctcg
      421 cgatctgatc acttgatcgg tcgcgtgtgt cgcgaccgga cggcgtcccc ctcgtgattg
      481 gaagcgcgaa ggggcaattt acggatgagt tcttggctta tactggccat agcggccggg
      541 ctgctggcgg tgctgtatgg cgccgtgcag acggccagct tgatgcgggc ctcgcccggc
      601 aatgcgaaga tgcaggagat cgccgcggcg atccaggaag gcgctcaggc ctatctgaac
      661 cgccagtaca ccaccatcgc cctggtcggc gtcgtggtca tcgcgcttct ggcgtttttc
      721 tttaagtcct gggagcagcc cgtcggcttc gccgcaggct ttgccggcat gctgatctcg
      781 gtccgcgcca atgtgcgcac ggcccaggcc tcgtccgaga gcctggccaa gggcctgtcg
      841 atggccttca cctccggcgc cgtaaccggc atgctggtgg ccggcttcgc cctgatcggc
      901 gtggcgggct atttcgccct gctgaccggt cctggaggct atgccggcac cgaccgcgtg
      961 gtggttgatt cgctggtctc gctgggcttc ggcgccagcc tgatctcgat cttcgcccgt
     1021 ctgggcggcg gcatcttcac caagggcgcc gacgtgggcg gcg
//
LOCUS       DAOMEO010001272         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14046_length_1059_cov_2.143426, whole genome shotgun sequence.
ACCESSION   DAOMEO010001272 DAOMEO010000000
VERSION     DAOMEO010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14046_length_1059_cov_2.143426"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="PLH31_20380"
     CDS             complement(<1..>1059)
                     /locus_tag="PLH31_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HQR91584.1"
                     /translation="RGYMDYVGVRRYGADGKPSGEVRFVGLFTAEAYETPAHDVPLIR
                     RKVAHVLASAGKDPDSHNGKRLRNILETWPRDELFHADEAVLLAMALGVLHLYDRPRV
                     RLFTRRDPFDRFISALLFVPRERYDSGVRERAGKLLAEAFGGRVSAYYPSFSDAPLAR
                     VHFIIGVTPGDHREPDMAALEDAIAETARTWEDRFEAAIREGGAPGRVAQTLARYAGA
                     FPPGYRDQYDAAEALADIEVVDALAEDEAVRVRAFRRPGDTATTFRFKLYRPGAAAPL
                     ADVLPILEHMGLKAMIEDGHKVSPTSAEGQSTAVWVHEFMLVDEQGEHLSFADVKLAF
                     EAAFVAIWTGRAESDGFNR"
BASE COUNT          168 a          342 c          392 g          157 t
ORIGIN      
        1 gcggttgaag ccgtcgctct cggcgcggcc cgtccagatg gcgacgaagg cggcctcgaa
       61 ggccagtttc acgtcggcga aggatagatg ctcgccctgc tcgtcgacga gcatgaactc
      121 gtgcacccag acagctgttg actggccctc tgcgctggtt ggcgagacct tgtggccgtc
      181 ctcgatcatg gccttcaggc ccatatgctc gaggatcggc aggacgtcag ccaggggcgc
      241 ggccgcgccc ggtcgataaa gcttgaagcg gaaggtcgtc gcggtgtcgc cggggcggcg
      301 gaaggcgcgc acacgcaccg cctcgtcctc ggccagggcg tcgaccacct cgatatcggc
      361 cagggcctcg gcggcgtcgt attggtcgcg atagccgggc ggaaaggcgc cggcgtatcg
      421 ggccagggtc tgggccacgc gtcccggggc gccgccctcg cggatcgccg cctcgaagcg
      481 gtcttcccag gtgcgggcgg tctcggcgat ggcgtcctca agggcggcca tgtccggctc
      541 gcgatggtcg cctggcgtga cgccaatgat gaagtggacg cgcgccaggg gggcgtcgga
      601 gaagctggga taataggccg agacgcggcc gccaaaggcc tcggccagca gcttgccggc
      661 ccgctcgcgc acgccggaat catagcgctc gcgcggcacg aatagcaggg ccgagatgaa
      721 gcggtcgaaa ggatcgcggc gggtgaacag ccggacgcgc gggcggtcat agaggtgcag
      781 gacacccagc gccatggcca gcagcactgc ctcgtcggcg tggaacagct cgtcgcgcgg
      841 ccaggtctca aggatgttgc ggagacgctt gccgttgtgg ctgtcgggat ccttgccagc
      901 gctggccagc acatgcgcca ccttgcggcg gatcagcgga acgtcgtgag cgggggtctc
      961 ataggcttcg gcggtgaaca ggcccacgaa ccggacttcg cccgagggct tgccgtcggc
     1021 gccgtaccgg cgcaccccga catagtccat gtagccgcg
//
LOCUS       DAOMEO010001273         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14049_length_1059_cov_1.959163, whole genome shotgun sequence.
ACCESSION   DAOMEO010001273 DAOMEO010000000
VERSION     DAOMEO010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14049_length_1059_cov_1.959163"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLH31_20385"
     CDS             <1..916
                     /locus_tag="PLH31_20385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR91585.1"
                     /translation="APAPGLLSWAWINPISRLILPLLAFGLALGAVLYAAERAVIRWI
                     IYLQRIAAIYARGRFTVRPLQAERAPPEIRELAATLDDMAGAIVARDASLMDSLAEKD
                     ALMREIHHRVKNNLQIITSLLNMQQRALTDPAAKAAMNDTRQRITALAQIYRALYQGP
                     DLKRVDLRPFMEELTAQLLAGEMSGSGAVRTEVHADPLVIDPDRLAPIALFAVEAITN
                     AQKHAFAERGGILSVNFHVRGDEAELSIGDDGPGANQGPGEGVGRTLMSAFARQLRGK
                     VTFETSDQGGLLVRLVFPTPQAPPSAGT"
     gene            986..>1059
                     /locus_tag="PLH31_20390"
     CDS             986..>1059
                     /locus_tag="PLH31_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin EcnA/B family protein"
                     /protein_id="HQR91586.1"
                     /translation="MRKLIVLAAIAASLSVAACNTVEGA"
BASE COUNT          180 a          374 c          337 g          168 t
ORIGIN      
        1 ggcgccggcg ccgggcctgc tgtcctgggc ctggatcaat ccgatctcgc gcctgatcct
       61 gccgctcctg gccttcggcc tggccctggg ggcggtgctt tacgcggccg agcgcgccgt
      121 gatccgctgg atcatctatc tgcagcgcat tgcggcgatc tatgccaggg gtcgctttac
      181 cgtacggccc ctgcaggccg agcgggcgcc gcccgagatc cgcgaactgg ccgccaccct
      241 cgacgacatg gccggggcga tcgtggcgcg cgatgcgtcc ctgatggaca gcctggcgga
      301 gaaagacgcg ctgatgcgcg aaatccatca ccgggtgaag aacaacctgc agatcatcac
      361 ctcgctgctc aacatgcagc agcgggccct gaccgatccg gccgccaagg cggcgatgaa
      421 cgatacgcgc cagcggatca cggccctggc ccagatctac cgcgcgctct atcagggacc
      481 cgacctcaag cgggtggatc tgcggccttt catggaagag ctgacagccc agctgctggc
      541 cggcgaaatg tccggatcag gggcggtgcg caccgaggtc cacgccgatc ccctggtcat
      601 cgaccccgac cgcctggcgc cgatcgccct gttcgccgtt gaggccatca ccaacgccca
      661 gaagcacgcc tttgccgaac ggggcgggat tctgtcggtg aacttccatg tgcgcggcga
      721 tgaagccgaa ctgtccatcg gcgatgatgg accgggcgcc aatcagggcc cgggcgaagg
      781 cgtcgggcga accctgatga gcgctttcgc gcgacagctt cgcggcaagg tgaccttcga
      841 aaccagcgac cagggcggcc tgcttgtgcg cctggtgttc cccacgccac aagcgccgcc
      901 ttcggccgga acctagcttc gcccggggcg ttgtctgact cgaacaggtt ccatgcccca
      961 tcgggccaaa acaccggaga acaccatgcg caaattgatc gtactcgccg ccatcgccgc
     1021 cagcctttcc gtcgccgcct gcaacaccgt cgaaggcgc
//
LOCUS       DAOMEO010001274         1058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14056_length_1058_cov_2.721834, whole genome shotgun sequence.
ACCESSION   DAOMEO010001274 DAOMEO010000000
VERSION     DAOMEO010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_1058_cov_2.721834"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1058)
                     /locus_tag="PLH31_20395"
     CDS             complement(<1..>1058)
                     /locus_tag="PLH31_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR91587.1"
                     /translation="AAGLWNFFMPPHSGQTHVDDTFVFEGVQLTNLEYSLIAEQLGKV
                     GFASEVFNCSAPDTGNMEVLMRYGTLAQKERWLRPLMNGEIRSAFLMTEPDVASSDAT
                     NIETRIERDGDHYVINGRKWWSSGVGDPRCKVAIVMGKTDPDNASRHAQQSQVLVPLD
                     APGIEIVRMLPVFGYDDAPHGHAEVILKNVRVPIEDALLLGEGRGFEIAQGRLGPGRI
                     HHCMRTIGTAEVALEKMCKRLMSRKAFGKYISDHSVWEERVADARIDIEMCRLLCLKA
                     ADMMDKAGNKSARLEIAMIKVAAPRLALKIIDDAIQAHGGGGVTTDYGLAKAYSGIRT
                     LRLADGPDEVHQRTIARM"
BASE COUNT          150 a          337 c          374 g          197 t
ORIGIN      
        1 ccatgcgggc gatggtgcgc tggtgtactt cgtcggggcc gtcggccagg cgcagggtgc
       61 ggatgccgct gtaggccttg gccaggccgt agtcggtggt gaccccgccg ccgccgtggg
      121 cctggatggc gtcgtcgatg atcttcaggg ccaggcgcgg agccgcgacc ttgatcatgg
      181 cgatctccag acgggccgac ttgttgccgg ccttgtccat catgtcggcg gccttcaggc
      241 acagcaggcg gcacatctcg atgtcgatgc gggcgtcggc gacgcgctct tcccacaccg
      301 agtggtcgga gatgtacttg ccgaacgcct tgcggctcat caggcgcttg cacatctttt
      361 caagcgccac ctcggcggtg ccgatggtgc gcatgcaatg gtggatgcgg ccggggccca
      421 ggcggccctg ggcgatctcg aagccgcggc cttcgcccag cagcagggcg tcctcgatcg
      481 gcacgcggac gttcttgagg atcacctcgg cgtggccgtg cggggcgtcg tcatagccga
      541 acaccggcag catgcggacg atctcgatgc cgggcgcgtc cagcggcacc agaacctggc
      601 tctgctgggc gtggcggctg gcgttgtccg ggtcggtctt gcccatcacg atggcgacct
      661 tgcagcgcgg atcgcccacg cccgaggacc accacttgcg gccgttgatc acatagtggt
      721 cgccgtcgcg ctcgatgcgg gtctcgatat tggtggcgtc cgaggaggcg acgtcgggct
      781 cggtcatcag gaaggcgctg cggatctcgc cgttcatcag ggggcgcagc cagcgctcct
      841 tctgggccag cgtgccgtag cgcatcagca cttccatgtt gccggtgtcg ggcgccgagc
      901 agttgaagac ttccgaggcg aaacccacct tgcctagctg ttcggcgatc aggctgtatt
      961 ccagattggt cagttgcacg ccctcgaaga cgaacgtgtc gtccacatgg gtctggccgc
     1021 tgtgcggcgg catgaagaag ttccacaggc cggcggcc
//
LOCUS       DAOMEO010001275         1058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14057_length_1058_cov_2.448654, whole genome shotgun sequence.
ACCESSION   DAOMEO010001275 DAOMEO010000000
VERSION     DAOMEO010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14057_length_1058_cov_2.448654"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLH31_20400"
     CDS             <1..685
                     /locus_tag="PLH31_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQR91588.1"
                     /translation="EDGLPPVATVFSLSPIWLLPAALPALKARGMTRLVAFSSTSRFT
                     KQVSEVPEERAVAASLAQAEAALEQFCTSHGVAWTLLRPTLIYDEGHDGNVSRLASLI
                     RRFGVLPLSGKGEGLRQPVHAQDLAIGALAAATAAAAENRAYDLVGGETLTYHAMATR
                     VFEGLDRRPAVFSLPPALFSLMLKAAAPFLPGATAAMGARMGVDLTFDSRDAVRDFGW
                     APRAFHPKF"
     gene            complement(690..1031)
                     /locus_tag="PLH31_20405"
     CDS             complement(690..1031)
                     /locus_tag="PLH31_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR91589.1"
                     /translation="MREDGKIDYVEWPAADLPATKGFYEKAFGWTFTDYGPDYAAFDG
                     QGADGGFFRPEAAPPKPLVILYAHDLEAMEARVTAAGGVISTPIFSFPGGRRFHFTDP
                     AGNELAVWSET"
BASE COUNT          141 a          368 c          377 g          172 t
ORIGIN      
        1 ggaagacggc ctgccgccgg tggcgacggt gttttccctg tcgccgatct ggctgctacc
       61 cgccgccctg ccggcgctca aggcccgggg catgacccgt ctggtggcct tttcctcgac
      121 cagccgcttc accaagcagg tctcggaggt tcccgaggaa cgcgccgtgg ccgccagcct
      181 ggcgcaggcc gaggcggcgc tagaacagtt ctgcaccagc cacggcgtgg cctggaccct
      241 gctgcggccg accctgatct atgacgaggg ccatgacggc aatgtcagcc ggttggccag
      301 tctgatccgc cggttcgggg tgctgcccct gtcgggcaag ggcgagggcc tgcgccagcc
      361 ggtccacgcg caggacctgg ccatcggcgc cctggcggcg gcgaccgcag cggcggccga
      421 gaaccgggcc tatgacctgg tcggcggcga gaccctgacc taccacgcca tggcgacgcg
      481 ggtgtttgag gggctggacc ggcggccggc ggtgttttcg ctgcccccgg ccctgttcag
      541 cctgatgctg aaggccgccg cgccgttcct gcccggggcc acggcggcca tgggcgcgcg
      601 gatgggcgtg gacctcacct tcgacagccg cgacgccgtc cgcgatttcg gctgggcccc
      661 gcgcgccttt cacccgaagt tctgaggcct caggtctcgc tccacacggc cagctcgttg
      721 ccggccgggt cggtgaagtg gaagcggcga ccgcctggaa agctgaagat cggggtgctg
      781 atcacgccgc cggcggccgt gaccctggcc tccatggcct ccagatcatg ggcgtacagg
      841 atcaccagcg gcttgggcgg cgcggcctcg ggcctgaaga agccgccatc ggcgccctgg
      901 ccgtcaaagg cggcatagtc ggggccgtag tcggtgaagg tccagccgaa ggccttctcg
      961 tagaagccct tggtggcggg caggtcggcc gcgggccact cgacatagtc gatcttgccg
     1021 tcttcgcgca tggtcgcgat ctccagattt gttccact
//
LOCUS       DAOMEO010001276         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14099_length_1056_cov_2.817183, whole genome shotgun sequence.
ACCESSION   DAOMEO010001276 DAOMEO010000000
VERSION     DAOMEO010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14099_length_1056_cov_2.817183"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLH31_20410"
     CDS             complement(<1..461)
                     /locus_tag="PLH31_20410"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HQR91590.1"
                     /translation="MALRLEPGLKLVVATHNPGKVPEIAALLDGRFELVSAGALGLPE
                     PEETETTFVGNALLKARHAADLSGMIALADDSGLSVAALDGSPGVYSARWAGPGKDFA
                     QAMAKVEERLEETGSDDRAAWFTSALAIAWPGGPSVVVQGEVHGTLAFPPRG"
     gene            487..>1056
                     /locus_tag="PLH31_20415"
     CDS             487..>1056
                     /locus_tag="PLH31_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR91591.1"
                     /translation="MRLLYSALSPFARKVRIVAHENGFTDAIMLETVAPFTDESLRAT
                     NPLSQVPTLILDDGEILFDSSVICDHLDAEGGAGLIPAGGRDRLRALTLQALADGMGS
                     AAVAVVRERMRPEDDQRPELIARQTAAIVAGLDLLESEGLANDRWAIAEIAVAAQLAY
                     FDARKVLGWRDGRPALSAWFEAASRREAMV"
BASE COUNT          170 a          381 c          344 g          161 t
ORIGIN      
        1 ccgcgcggcg ggaaggccag ggttccgtgg acctcgccct gcaccaccac ggatgggcca
       61 cccggccagg cgatcgccag ggccgatgtg aaccaggcgg cgcgatcatc cgagccggtc
      121 tcctcgagac gttcctcgac cttggccatg gcctgggcaa aatccttgcc cgggccagcc
      181 cagcgcgctg aatagacacc gggcgagccg tccagcgccg ccaccgacag ccccgagtcg
      241 tcggccaggg cgatcatccc cgacaggtcg gcggcgtggc gcgccttcag cagcgcgttg
      301 ccgacaaagg tggtctcggt ctcttccggc tcaggcaggc ccagggcccc ggccgagacc
      361 agctcaaacc gcccatccag cagggcggcg atctcgggga ccttgccggg attgtgcgtg
      421 gccactacga gcttaagccc aggctccagt ctcagcgcca tgatcggctc cttgaggaaa
      481 acaccaatgc ggctccttta ctccgccttg tcgccgtttg cgcgcaaggt tcgcatcgtg
      541 gcccacgaaa acggcttcac cgacgcgatc atgcttgaga ccgtggcccc cttcaccgac
      601 gagagcctcc gcgcgaccaa cccgcttagc caggtgccga ccctgatcct cgacgacggc
      661 gagatcctgt tcgactcctc agtcatctgc gatcaccttg acgccgaagg cggcgcgggg
      721 ctgatcccgg cgggcggcag ggatcgtctc cgcgccttga cgctgcaggc cctcgccgac
      781 ggtatgggtt cggccgccgt cgccgtggtc cgtgagcgga tgcggccgga agacgaccag
      841 cgccccgaac tgatcgcgcg ccaaacggcc gcgatcgtcg cgggcctcga cctgctggaa
      901 tccgaggggc tcgccaacga tcgatgggcg atcgcggaaa tcgccgtggc cgcgcaactg
      961 gcctatttcg acgcccgcaa ggtcctcggc tggcgcgacg gccgacctgc cctgagcgct
     1021 tggtttgaag ccgcgagccg gcgcgaagct atggtt
//
LOCUS       DAOMEO010001277         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14111_length_1056_cov_1.585415, whole genome shotgun sequence.
ACCESSION   DAOMEO010001277 DAOMEO010000000
VERSION     DAOMEO010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14111_length_1056_cov_1.585415"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..>395)
                     /locus_tag="PLH31_20420"
     CDS             complement(164..>395)
                     /locus_tag="PLH31_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91592.1"
                     /translation="ALLTGHGLLKRPLGFVAAFGFVLGAALFAGDLAMRQYQGRSLFA
                     MAAPTGGTTLILSWLVLAIAAAWPQPPLKPEA"
     assembly_gap    396..405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(612..>1056)
                     /locus_tag="PLH31_20425"
     CDS             complement(612..>1056)
                     /locus_tag="PLH31_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91593.1"
                     /translation="ELIFFEGPPSPDGGKEASLRLVDMPGYGYASAPKAKIASWTTLI
                     HKFLQGRASLARVYVLIDSRHGFKDVDTQVLTTLDKSAVSYQAVLTKCDQVKKSELEQ
                     TLLAVTETLRKHPAAFPDILVTSSRTGEGMGELRAAMVRLLRERA"
BASE COUNT          194 a          323 c          342 g          187 t
ORIGIN      
        1 aatggtttcc tcgtcgtact gctgcatatg cggcagggct tcggagagta tccgggcctg
       61 atcctgaggg ctgatatcgg gcagggcgat catggaaaaa tctcgtgtgg cggctggcga
      121 tgtttagcgg atcggcatga caggcgcaaa gccgaagaca attttaggct tcaggtttta
      181 acggcggttg gggccatgcc gccgcgattg cgagcaccag ccagctcagg atcagcgtgg
      241 tgccgccggt tggcgccgcc atcgcaaaca gactccgtcc ctgatattgg cgcatcgcca
      301 gatcgcccgc gaacagggcc gcgcccaata caaacccgaa ggccgccacg aagccgagcg
      361 ggcgcttcag caggccgtgg cccgtcagca gggcgnnnnn nnnnnggccc cgatgacagc
      421 gctggcatgg aacagcagca ttgaagacgc cgaccccagc cgcgccgcat cgggctggtg
      481 cgcggcggcg gcggcgagaa tcacaccggc cgcgcccatc aggccggcgc agacggcggc
      541 aatgcgaagc agggcggttt ggatcatcgg gtgtctttcc gtttgggcat gatgttatcc
      601 gctttactgg atcacgcccg ctccctcaaa agccgcacca ttgcggcgcg cagttcgccc
      661 atgccttcgc cggtgcgcga cgacgtcacc aggatgtcgg gaaaggcggc gggatgcttg
      721 cgcagcgtct cggtgaccgc gagcagggtc tgttcgagtt cgcttttctt aacctggtcg
      781 catttggtca gcaccgcctg atagctcacc gccgacttgt ccagcgtggt caacacctgc
      841 gtatcgacgt ccttgaagcc gtggcgcgag tcgatcagca catagacgcg ggcgagactg
      901 gcacggcctt gcagaaattt atggatcagc gtggtccatg acgcgatctt cgctttcggc
      961 gccgacgcat aaccatagcc cggcatatcg acgaggcgca ggctggcctc cttgccgccg
     1021 tcgggcgaag gcgggccctc gaagaaaatc agctcc
//
LOCUS       DAOMEO010001278         1055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14126_length_1055_cov_1.859000, whole genome shotgun sequence.
ACCESSION   DAOMEO010001278 DAOMEO010000000
VERSION     DAOMEO010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14126_length_1055_cov_1.859000"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLH31_20430"
     CDS             <1..254
                     /locus_tag="PLH31_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQR91594.1"
                     /translation="WRARFSLDVEPLGPVHVQIALTGACTRVGVWAERPDAMARLQAG
                     EAALSAALREAELSPEVAFHAGSPTVAATEPGHFVDQAS"
     assembly_gap    287..296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            302..529
                     /locus_tag="PLH31_20435"
     CDS             302..529
                     /locus_tag="PLH31_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQR91595.1"
                     /translation="MAVALFYEGVDAPKVVASGQGWLGDKIVETAREHGVPIEENPAL
                     AQALSTIEIDEEIPEALYRAIAEILSFLLKP"
     gene            complement(590..>1055)
                     /locus_tag="PLH31_20440"
     CDS             complement(590..>1055)
                     /locus_tag="PLH31_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQR91596.1"
                     /translation="EMLEEIGTVENIPAYVQGVKDKKYKLMGFGHRVYKNFDPRAKVM
                     QKTCYEVLEQLGINDPLLQVAMELEKVALSDPYFIDRKLYPNIDFYSGITLRAMGFPT
                     NMFTVLFALARTVGWISQWKEMFEDPTRKIGRPRQLYTGATQRDYLPLEKRG"
BASE COUNT          167 a          326 c          348 g          204 t
ORIGIN      
        1 cctggcgcgc ccgcttctcg ctggatgtcg agccgctggg gccggttcat gtgcagatcg
       61 cgctcacggg cgcctgcacg cgcgtggggg tctgggccga gcgtccggac gccatggcgc
      121 gccttcaggc cggagaggcg gctttgagcg ccgcccttcg cgaggccgag ctgtcgccgg
      181 aggtcgcgtt ccacgcgggc tcgccgaccg tggctgcgac agagccaggc cactttgtgg
      241 atcaggcctc atgaccgggt cgatcggtgg cgtgcggggc cgcggcnnnn nnnnnngcac
      301 gatcgcggtc gccctattct acgaaggcgt cgatgcgccc aaggtcgtgg cgtccggcca
      361 gggctggctt ggcgacaaga tcgtcgagac ggcgcgcgaa cacggcgttc ccattgagga
      421 gaacccggcc ctggcccagg ccctgtcgac catcgagatc gacgaggaaa tccccgaggc
      481 tctctatcgc gcgatcgccg agatcctcag ttttctgctc aagccgtagc cggcaaagaa
      541 aaagccccgt cctgcgacgg ggcttttcca gatcctttcg gatcgatctt tagccgcgct
      601 tttcgagcgg caggtagtcg cgttgcgtcg cacccgtata aagctggcgc gggcggccga
      661 tcttgcgggt gggatcctcg aacatttcct tccactggct gatccagcca acggtgcggg
      721 ccagggcgaa cagcacggtg aacatgttgg tcgggaagcc catcgcgcgc agggtgatgc
      781 ccgaatagaa gtcgatgttc ggatagagct tgcggtcgat gaagtaagga tcgctgaggg
      841 cgaccttttc cagctccatg gccacctgca gcagcgggtc gttgatgccc agctgctcaa
      901 gcacttcgta gcaggtcttc tgcatgacct tggcgcgggg gtcgaagttc ttgtacacgc
      961 ggtggccaaa gcccatcagc ttgtacttct tgtccttcac gccctgcaca taggctggga
     1021 tgttctcgac cgtgccgatt tcctcgagca tctcg
//
LOCUS       DAOMEO010001279         1054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14142_length_1054_cov_2.533534, whole genome shotgun sequence.
ACCESSION   DAOMEO010001279 DAOMEO010000000
VERSION     DAOMEO010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14142_length_1054_cov_2.533534"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1054)
                     /locus_tag="PLH31_20445"
                     /pseudo
     CDS             complement(1..1054)
                     /locus_tag="PLH31_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611538.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          182 a          340 c          308 g          224 t
ORIGIN      
        1 gcggatgata gggcgcgccg cgaacgtgtc ccatgttctg tgcatccagc cagtttgcaa
       61 gctcacccga tacgtagctg gaaccattgt cgctgagcag gcgcggctta tggacaaccc
      121 gcgcctgatc gcagcctgag gccctaagag caagatccag cgtgtcggtc acgtcctgcg
      181 ccttcatcgt cgtgcacagc ttccacgcga tgatgtaacg cgagaagtcg tccagcaccg
      241 tgctgagata gaaccagccc cagccagtca ccttgagata ggtgaagtcc gtctgccaga
      301 gctggttggt cgccgtcgtc ttgtccttga actcgttggc cgccttcatg acgatgtagg
      361 ctgggctggt gatcaggtca tgcgccttaa gcagccgata gaccgaagct tctgacacaa
      421 agtaattggc cgtatcggta aagcgcgtgg ccagttcccg tggcgatagc tcgggctcat
      481 ccagggccag ttgaacgatc cgttcgcgta tggcgtcagg gattcggttc cagacgcgct
      541 tcggccttgg cgccttgtct tccagcgcct cgggcccgcc ggtctggagc cgatcgtacc
      601 agcggtagaa ccgtgaaggc tggatcccca gcttctccac cgtttggcgc accggcagat
      661 gcgattgctc aaccagccgg atgatctcaa gcttctcgga ggcgggatat ctcattcctc
      721 gtctccccca tccgcgatca tgcttttttt aagcaggcgc aattcgagcg cctgctcagc
      781 gacgactgcc ttgagctgct gcgcttcacg ccgcagatcc tttacctcgc cgctggtcgc
      841 ggcccgcgcc gtatcgccca ccagacgccg cttgccggcc tccaggaact ccttggacca
      901 ggcgtaatag aggctctcgg caatgccttc gcggcgacac agctcggcga tgctggtctc
      961 gccccgcagg ccgtccagaa cgatccggat cttctcttcg ggcgaatgcc gcttgcgggt
     1021 tgcccggcgg atgttcttca cgatccgctc ggac
//
LOCUS       DAOMEO010001280         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14182_length_1052_cov_2.198596, whole genome shotgun sequence.
ACCESSION   DAOMEO010001280 DAOMEO010000000
VERSION     DAOMEO010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_1052_cov_2.198596"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLH31_20450"
     CDS             <1..406
                     /locus_tag="PLH31_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQR91597.1"
                     /translation="IEFYYKEDKLNDPMVSEEHITAFNWAATQEIDDMMAMALRVNDY
                     LSGLFSGVGITLVDFKIEFGRIYEGDFSRVILADEISPDSCRLWDSITNEKMDKDRFR
                     RDLGNVIESYTEVARRLGIMKEMPTVIQGGMH"
     gene            571..>807
                     /gene="purS"
                     /locus_tag="PLH31_20455"
     CDS             571..>807
                     /gene="purS"
                     /locus_tag="PLH31_20455"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQR91598.1"
                     /translation="MKAKVQVFLKPGVLDVQGKAVEAALHGLGWTDVQNVRVGRTIEF
                     DVADGDADKAQAEVKAMCDKLLANTVIETYRVEFA"
     assembly_gap    808..817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(863..>1052)
                     /locus_tag="PLH31_20460"
     CDS             complement(863..>1052)
                     /locus_tag="PLH31_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR91599.1"
                     /translation="EAMFLRGQAEGVIRQGLNADAAALMVGALLLGLSTQMLVDPGLD
                     LEPIRETSLMTLRVSFSA"
BASE COUNT          217 a          328 c          315 g          182 t
ORIGIN      
        1 catcgaattc tactacaagg aagacaagct caacgacccg atggtgtcgg aagagcacat
       61 caccgccttc aactgggccg ccacccagga gattgacgac atgatggcca tggccctgcg
      121 ggtgaacgac tatctgtcgg gcctgttcag cggcgtcggc atcacgctgg tggacttcaa
      181 gatcgagttt ggccgcatct atgaaggcga cttcagccgc gtgatcctgg ccgacgagat
      241 cagccccgac agctgtcgcc tgtgggactc gatcaccaac gaaaagatgg acaaggaccg
      301 cttccgtcgc gacctgggca atgtcatcga aagctatacc gaggtggccc gccgcctggg
      361 tatcatgaag gaaatgccca ccgtcattca gggcggcatg cactaagaac acaccttctc
      421 ccatagggag aaggaggggc ccacgccttt ggcgtgggag gatgaggggt taaaccccgt
      481 ccggttggcc ccgtaacccc tcaccctccc atgctgcgca tgggcccctc cctctcccta
      541 tgggagaggg tgaaaaaggt ttgaagcgct atgaaagcca aggtccaagt gttcctcaag
      601 cccggcgttc tcgacgttca gggcaaggcc gtcgaagccg ccctgcatgg gctgggctgg
      661 acagatgtgc agaacgttcg cgtcggccgc accatcgagt tcgacgtcgc tgacggtgac
      721 gccgacaagg cccaggccga ggtcaaggcc atgtgcgaca agctgctggc caacaccgtc
      781 atcgagacct accgggtcga gttcgcgnnn nnnnnnnatc gagagctacg cgatcaccct
      841 cgactaagcg tctttcggcg tttcaggcgc tgaagctcac gcgcagggtc atcaggctgg
      901 tttcccggat cggttcaaga tcgagaccgg gatccaccag catctgggtg ctgagcccca
      961 gcagcagggc cccgaccatc agggcggccg catcggcgtt gagcccctgg cggatcacgc
     1021 cctcggcctg ccctctcagg aacatggcct cc
//
LOCUS       DAOMEO010001281         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14193_length_1052_cov_1.397192, whole genome shotgun sequence.
ACCESSION   DAOMEO010001281 DAOMEO010000000
VERSION     DAOMEO010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_1052_cov_1.397192"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLH31_20465"
     CDS             complement(<1..397)
                     /locus_tag="PLH31_20465"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR91600.1"
                     /translation="MARDSAVSEKRPLRVGVTGIGVMGSNHARVLAELPGAELVAIAD
                     PDQAQAARVAQFLGCQAVGDHNDLLALGLDAVIVAAPTHLHHPIALDVINAGCSVLVE
                     KPVASTVEQGREIVSLARTKGVTLMIGHVE"
     gene            complement(561..>1052)
                     /locus_tag="PLH31_20470"
     CDS             complement(561..>1052)
                     /locus_tag="PLH31_20470"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQR91601.1"
                     /translation="IGANTGYAWGSGTGAADAYGVNPDGWLGGGQIGYNFQFQNNVLI
                     GLEADVDAANISDSNGPVSSKVDYFGTVRARLGYAFDRVLPYVTGGLAWGNNKVTANG
                     VSQSNTSTGWAAGAGIEYALTNNWTARAEYLYLDLGKSGFQSVGDSGVTASTARLGVN
                     YKF"
BASE COUNT          194 a          340 c          342 g          176 t
ORIGIN      
        1 gctccacatg gccgatcatc agggtcacgc ccttggtgcg ggccagggag acgatctcgc
       61 gcccctgctc caccgttgat gcgaccggct tctccaccag gactgagcag ccggcattga
      121 tgacgtcgag ggcaatgggg tggtgcagat gcgtgggggc ggcgacgatc accgcatcaa
      181 ggccgagcgc cagcaggtca ttgtggtcgc ccaccgcctg acagcccagg aactgcgcga
      241 ctcgggccgc ctgcgcctga tcgggatcgg cgatcgccac cagttccgcg ccaggcaatt
      301 cggccagcac gcgggcatga ttcgacccca tgacgccgat gccggtgaca ccaacgcgca
      361 ggggacgctt ctcggacaca gccgaatcac gagccatttc agccctctta gtcatgacgt
      421 tcgggctggc tcgtatcacg ccaatgcgca ccaaagcgag gcagggtggc ccgggaagcg
      481 gggcggggcg ttcaacaatt gcatcgccgt gttaccaccc cggcccgcgc gaggccggag
      541 agccagaggg atggggcggc ttagaacttg tagttcacgc ccaatcgggc ggtggatgcg
      601 gtcacgcccg agtcgccaac ggactgaaaa ccgctcttgc cgagatcgag atagagatat
      661 tcggcgcggg cggtccagtt gttggtcagc gcgtactcga tacctgcgcc tgccgcccag
      721 ccggtcgagg tgttggattg gctcacgcca ttggccgtga ccttgttgtt gccccaagcc
      781 aaaccgccgg tgacataggg cagaacccga tcaaaggcgt agcccagccg cgcgcgcacc
      841 gtgccgaaat agtccacttt cgaggacacc ggcccattgc tgtcgctgat gttggcagca
      901 tccacatcgg cttcgaggcc gatcagcaca ttgttctgga actggaagtt gtaaccgatc
      961 tgtccgccgc cgagccaacc gtcggggttg acgccgtaag cgtccgcagc cccggtgccg
     1021 gagccccagg catagccggt gttggcgccg at
//
LOCUS       DAOMEO010001282         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14206_length_1051_cov_1.969880, whole genome shotgun sequence.
ACCESSION   DAOMEO010001282 DAOMEO010000000
VERSION     DAOMEO010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_1051_cov_1.969880"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="PLH31_20475"
     CDS             <1..>1051
                     /locus_tag="PLH31_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018081110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQR91602.1"
                     /translation="FIEIWNLVFMQFEQLAGGERLSLPKPSIDTGMGLERVAAVLQGV
                     HDNYDIDLFKALIAASVELTGVKAEGPQAPSHRVIADHLRSSSFLLADGVTPSNEGRG
                     YVLRRIMRRAMRHAYLLGANEPLMHRLAPTLVAEMGQAYPELRRAEASIVETLRQEEE
                     RFRVTLGRGMGLLDEATANLSSGGVLDGEVAFKLYDTYGFPLDLTQDAVRAKGITVDT
                     DGFDVAMDRQRTMARANWSGSGQQGAAAAWFGIKERTGATDFVGYDTTETTGVVKAIL
                     ADGAEVDAASAGQTVEVLLDRTSFYAESGGQAGDTGVIEAHGRESRVLDTQKQAGELY
                     VHRVELAGDLKVGDGV"
BASE COUNT          169 a          371 c          351 g          160 t
ORIGIN      
        1 gttcatcgag atctggaatc tggtcttcat gcagttcgag cagctggccg gcggcgagcg
       61 cctgtcgctg cccaagccct cgatcgacac cggcatgggt ctggagcgcg tcgccgccgt
      121 cctgcagggc gtgcacgaca actatgacat cgacctgttc aaggccctga tcgccgccag
      181 tgtcgaactg accggcgtca aggccgaggg cccccaggcc ccgtcgcacc gggtgatcgc
      241 cgaccacctg cgctcgtcct cgttcctgct ggccgacggc gttacgcctt cgaacgaagg
      301 tcgcggctac gtcctgcgcc ggatcatgcg ccgcgccatg cgccacgcct atctgctggg
      361 cgccaatgag cccctgatgc accgcctggc gccgacccta gtggccgaga tgggccaggc
      421 ctatcccgaa ctgcgccgcg ccgaggcctc gatcgtcgag accctgcgcc aggaggaaga
      481 gcgcttccgc gtcaccctgg gtcgcggcat gggcctgctg gatgaagcca ccgccaacct
      541 gtcttcgggc ggggtgctgg atggcgaagt cgccttcaag ctctacgaca cctacggctt
      601 cccgctggac ctgacccagg acgccgtgcg cgccaagggc atcacagtcg acaccgacgg
      661 cttcgacgtg gccatggacc gccagcgcac catggcccgc gctaactggt cgggctcggg
      721 ccagcagggc gcggccgccg cctggtttgg catcaaggaa cgcacgggcg cgaccgactt
      781 cgtgggctac gacaccaccg agactaccgg cgtcgtgaag gcgatcctgg ctgatggcgc
      841 cgaggtcgac gctgcgtcgg ccggtcagac cgtcgaggtc ctgctggatc gcaccagttt
      901 ctacgccgag agcggcggcc aggctggcga cacgggcgtg atcgaagccc acggccgcga
      961 gagccgggtg ctagacaccc agaagcaggc cggcgagctc tatgtccacc gcgtcgaact
     1021 ggccggcgac cttaaggtcg gcgacggcgt c
//
LOCUS       DAOMEO010001283         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14229_length_1050_cov_1.819095, whole genome shotgun sequence.
ACCESSION   DAOMEO010001283 DAOMEO010000000
VERSION     DAOMEO010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_1050_cov_1.819095"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLH31_20480"
     CDS             complement(<1..553)
                     /locus_tag="PLH31_20480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR91603.1"
                     /translation="MSKLAVVAAGGTGGHMFPAQALAEALAARGWRVVLATDDRGALY
                     ADKFPAEERLALSAATAKAGDPIGMLRAGFVVAQGVLQARAAFKRLEPAVVVGFGGYP
                     ALPALLGALSQGRPTVIHEQNAVLGRVNRFLAPRVNEVACAFPILEKATPAVKARAHV
                     VGNPVRPPVRALFDVPYLPPEVQL"
     gene            complement(555..>1050)
                     /locus_tag="PLH31_20485"
     CDS             complement(555..>1050)
                     /locus_tag="PLH31_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQR91604.1"
                     /translation="QKFLSPDQADTHQISRAAEAIHAGGLFGRGPGEGVMKRHVPDLH
                     TDFIYSVAAEEYGLVFSLGLISLFAFVVVRGLMKAMKLNDTFEQVAAAGLFVLVGQQA
                     MINIAVNLNMIPTKGMTLPFISYGGSSMLAMGLTLGLALALLRKRPGAYGPSNEFMQG
                     GAYQ"
BASE COUNT          169 a          345 c          383 g          153 t
ORIGIN      
        1 gcagctgcac ttccggcggc aggtagggca cgtcgaagag ggcgcgcacg ggcgggcgca
       61 cggggttgcc gacgacgtgg gcgcgggcct tcacggcggg cgtggccttt tccaggatcg
      121 ggaaggcgca ggccacctcg ttgacccggg gggccaggaa acggttgacc cggcccagca
      181 ccgcgttctg ctcgtggatc accgtggggc ggccctggct gagggctccc agcagggcgg
      241 gaagcgcggg atagccgcca aagcccacca ccacggccgg ttccaggcgc ttgaaggcgg
      301 cgcgggcctg cagcacgcct tgggccacca caaagccggc cctgagcatg ccgatggggt
      361 cgccagcctt ggcggtggcg gccgagaggg ccaggcgttc ctcggcgggg aacttgtcgg
      421 cgtacagcgc gccgcgatcg tcggtggcca gcaccacgcg ccagcctcgg gcggccaggg
      481 cctcggccag ggcctgggcg gggaacatgt gaccgccggt tcctccggcg gcgacgacgg
      541 cgagcttgct catcttactg gtacgcgccg ccctgcatga actcgttgga cgggccatag
      601 gcgccggggc gcttgcgcag cagggccagg gccaggccca gggtcagtcc catcgcgagc
      661 atcgaagatc ccccatagct gatgaatggc agcgtcatgc ccttggtcgg gatcatgttc
      721 aagttcaccg cgatattgat catggcctgc tggccgacca gcacgaacaa accggcggcg
      781 gccacctgtt cgaaggtgtc gttcagcttc atggccttca tcaggccgcg caccaccaca
      841 aaggcgaaca ggctgatcag gcccagcgag aagaccaggc catattcctc ggccgcgacc
      901 gaatagatga agtcagtgtg caggtcgggc acgtggcgct tcatcacgcc ctcgcccggt
      961 ccgcgcccga acaggccgcc ggcgtggatg gcctcggcgg cgcggctgat ctgatgggtg
     1021 tcggcctggt cggggctgag gaacttctgc
//
LOCUS       DAOMEO010001284         1049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14240_length_1049_cov_1.935614, whole genome shotgun sequence.
ACCESSION   DAOMEO010001284 DAOMEO010000000
VERSION     DAOMEO010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_1049_cov_1.935614"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLH31_20490"
     CDS             <1..591
                     /locus_tag="PLH31_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR91605.1"
                     /translation="GFGKAIYALPDSYRRLSDGEDFDIGGRTWRVVTGQGHSPDHACL
                     WCPELDVLISGDQVLPRISSNVSVFPTEPEADPLSDWLRSLAKVKATVPDSVLVLPAH
                     NDPFHGLHARIDHLINGHERGLGRLQGKLEEPRRAIDVFGALFARPIGPDLLGMATGE
                     ALAHLNCLIGRGVVKRETDAEGVAWYAATEVTETTV"
     gene            623..>1049
                     /locus_tag="PLH31_20495"
     CDS             623..>1049
                     /locus_tag="PLH31_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQR91606.1"
                     /translation="MPVPPDRRLVFLLNAGNRRVQRWIEAAMAAKGGLTAAHSGVLFF
                     LGQRDGALIGEAAEALDLAPSAMTGLIDRMTKVDLVERRADPADGRAMRLYLTDKGRQ
                     AREYAKGGLDGINATLTEGFSDEEIAVVARWLMSLQTKFP"
BASE COUNT          161 a          359 c          365 g          164 t
ORIGIN      
        1 ggcttcggaa aggcgatcta cgccctgccc gacagctatc gccggctgtc cgacggcgag
       61 gacttcgaca tcggcggccg gacctggcgg gtggtgacgg ggcaggggca ttcgcccgac
      121 cacgcctgcc tgtggtgccc ggaactggac gtcctgatca gcggcgacca ggtgctgccg
      181 cgcatctcgt ccaatgtctc ggtgttcccg accgagcccg aggccgaccc gctgagcgac
      241 tggctgcgct cgctggccaa ggtcaaggcg acggtgcccg acagcgtgct ggtgctgccg
      301 gcccacaacg atccgttcca cggcctgcat gcccgcatcg accatttgat caacggccac
      361 gagcgaggcc tgggccggct tcagggcaag ctggaggagc ccaggcgggc catcgacgtg
      421 ttcggggccc tgttcgcccg gccgatcggt ccggacctgc tgggcatggc gacgggggag
      481 gccctggccc acctcaactg cctgatcggt cggggcgtgg tgaagcgcga gacggacgct
      541 gagggcgtgg cctggtacgc cgcgaccgag gtcacggaaa caaccgtttg atatttcgcg
      601 cgcgaagtat tagcgtcgcg ccatgcccgt tccgcccgat cgccgtctcg tctttctgct
      661 caacgccggc aaccggcgcg ttcagcgctg gatcgaggcg gcgatggcgg ccaagggcgg
      721 gctgacggcg gcccattccg gcgtgctgtt ctttctgggc cagcgcgacg gggccctgat
      781 cggcgaggcc gccgaagctt tggacctggc cccctcggcc atgaccggcc tgattgaccg
      841 gatgaccaag gttgatctgg ttgagcgccg cgccgacccc gccgacggcc gcgccatgcg
      901 gctgtacctc accgacaagg gccgccaggc gcgtgaatac gccaagggcg gcctggacgg
      961 catcaacgca accctgaccg aaggcttctc cgacgaggag atcgccgtgg tcgcccgctg
     1021 gctgatgagc ctgcaaacca agttcccca
//
LOCUS       DAOMEO010001285         1048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14255_length_1048_cov_2.804632, whole genome shotgun sequence.
ACCESSION   DAOMEO010001285 DAOMEO010000000
VERSION     DAOMEO010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14255_length_1048_cov_2.804632"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLH31_20500"
     CDS             complement(<1..326)
                     /locus_tag="PLH31_20500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQR91607.1"
                     /translation="MSPASPSTRCALTAPNRTPRRFRSRDWFDNPDHIDMTALYLERF
                     MNYGITPEELRSGKPIIGIAQTGSDISPCNRIHLDLVTRVRDGIRDAGGIPMEFPVHP
                     IFENCRR"
     gene            complement(280..>1048)
                     /locus_tag="PLH31_20505"
     CDS             complement(280..>1048)
                     /locus_tag="PLH31_20505"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQR91608.1"
                     /translation="RYDPATGAQQSYKAPDQVTFVAPIAGEEGFIAGLKSGLHSFDPE
                     TGDFAFLGEIEPPELDNRPNDSTVDAHGRLWFGTMHDGEEAASGALYRLTSAGETVKQ
                     DDGICITNGPCLSPDGKTFYHTDTLEKTIWAYDVDADGDLTNKRELCRIDIENAWPDG
                     SVVDSQGYLWTALWGGASVLRISPDGQVVDRIALPAPNVTKPCFGGPRLKTLYFTTAR
                     KGLSDETLAAWPEAGGLFAVDVDVAGQPQYEVRLDRP"
BASE COUNT          157 a          315 c          388 g          188 t
ORIGIN      
        1 cgacggcagt tctcgaagat cgggtggacc gggaactcca tcggaatgcc gccggcgtcg
       61 cggatgccgt cgcgcacgcg ggtcaccagg tccagatgga tgcggttgca cggcgagatg
      121 tcgctgccgg tctgggcgat gccgatgatc ggcttgccgc tgcgcagctc ctcgggcgtg
      181 atcccgtagt tcatgaaccg ctccagatag agggcggtca tgtcgatatg gtcgggattg
      241 tcgaaccagt cgcgcgagcg gaaccggcgg ggcgtgcggt taggggcggt caaggcgcac
      301 ctcgtactgg ggctggccgg cgacatcgac atcgacggcg aacaggccgc cggcttcggg
      361 ccaggcggcg agggtctcgt cgctaaggcc cttgcgggcg gtggtgaaat agagggtctt
      421 cagacgcggg ccgccaaagc acggcttggt gacattgggg gcgggcaggg cgatgcggtc
      481 gacgacctgg ccgtccggcg agatgcgcag caccgacgcg ccgccccaca gggcggtcca
      541 gagatagccc tggctgtcga ccaccgagcc atccggccag gcgttttcga tgtcgatgcg
      601 gcacagctcg cgcttattgg tcaggtcgcc atcggcgtcg acgtcatagg cccagatcgt
      661 cttttccagc gtgtcggtgt ggtagaaggt cttgccgtcc gggctcaggc acgggccgtt
      721 ggtgatgcag atgccgtcgt cctgctttac cgtctcgcca gcgctggtca ggcgatagag
      781 ggcgccgctg gcggcttctt cgccgtcgtg catggtgccg aaccacagcc ggccgtgggc
      841 gtcgacggtc gaatcgttgg ggcggttatc cagttctggg ggctcgattt cgcccaggaa
      901 ggcaaagtcg cccgtctcgg gatcgaagct gtgcaagccg ctcttcagcc ccgcgatgaa
      961 gccctcctcg ccggcgatgg gcgcgacgaa ggtcacctgg tcgggggcct tatagctctg
     1021 ctgcgcgcca gtggcagggt cgtagcgg
//
LOCUS       DAOMEO010001286         1047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14278_length_1047_cov_2.485887, whole genome shotgun sequence.
ACCESSION   DAOMEO010001286 DAOMEO010000000
VERSION     DAOMEO010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_1047_cov_2.485887"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLH31_20510"
     CDS             <1..988
                     /locus_tag="PLH31_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR91609.1"
                     /translation="AGFDIHPTLSIGAVNAEGGEDAPEATELLRRAELAVEAASAAGR
                     GGAAAYGRSMETDGLSRLALEADLRGAIARGEITPYFQPVVRLSTGALSGFEALARWL
                     HPRRGMLPPDEFLPLIEEMGLMSELGEHMMRTAGAQLAAWRRSHPAVGALTVSVNLST
                     GEIDRPGLVHDVAEVLRVNNLPRGALKLEVTESDIMRDPERAAVILKTLRDAGAGLAL
                     DDFGTGFSSLQYLTRLPFDTLKVDRYFVRTMGSNPGSAKIVRSVVKLGQDLDLEVVAE
                     GVENAEMAAALQALGCDYGQGFGYAPALSPQEAEVYLNEAYVDGAAPVKARG"
BASE COUNT          146 a          371 c          373 g          157 t
ORIGIN      
        1 ggccggcttc gacatccatc cgaccctgtc gatcggggcc gtcaacgccg agggcggcga
       61 ggacgccccc gaggccaccg aactgctgcg gcgcgccgaa ctggccgtcg aggcggcctc
      121 ggccgccggt cgcggcggcg cggccgccta tggccgctcg atggagaccg acggcctctc
      181 ccgcctggcg ctggaggccg acctgcgcgg cgccatcgcg cgcggcgaga tcacccccta
      241 tttccagccc gtcgtgcgcc tgtcgaccgg cgcgctgtcg ggcttcgagg ccctggcccg
      301 ctggctgcac ccgcgtcgcg gcatgctgcc gcccgacgaa ttcctcccgc tgatcgagga
      361 aatgggcctg atgagcgagc tgggcgagca catgatgcgc accgccggcg cgcagctggc
      421 cgcctggcgc cgcagccatc cggccgtggg cgccctgacc gtcagcgtca atctgtcgac
      481 cggcgagatc gaccgcccgg gcctggtgca tgacgtggcc gaggtgctgc gggtcaacaa
      541 cctgccgcgc ggcgccctga agctggaagt caccgaaagc gacatcatgc gcgaccccga
      601 gcgggccgcc gtgatcctca agaccctgcg cgacgccggc gccggcctgg ccctcgacga
      661 tttcggcacc ggcttctcgt cgctgcagta tctgacgcgc ctgccgttcg acaccctgaa
      721 ggtcgaccgc tatttcgtcc gcaccatggg cagcaatccg ggctcggcca agatcgtccg
      781 ctcggtggtg aagctgggtc aggacctgga cctggaagtc gtcgccgaag gcgtcgagaa
      841 cgccgagatg gcggctgcgc tgcaggcgct gggctgcgac tatggccagg gctttggcta
      901 tgcgccggcc ttgtcgccgc aggaggccga ggtttacctc aacgaggcct atgtggatgg
      961 cgcggcgccg gtgaaggcgc ggggttagtc ttcttagatc cccccccact gggggaggtg
     1021 gcccagaggg ccggagggtg ctaactg
//
LOCUS       DAOMEO010001287         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14304_length_1046_cov_2.243189, whole genome shotgun sequence.
ACCESSION   DAOMEO010001287 DAOMEO010000000
VERSION     DAOMEO010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14304_length_1046_cov_2.243189"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1046)
                     /locus_tag="PLH31_20515"
     CDS             complement(<1..>1046)
                     /locus_tag="PLH31_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HQR91610.1"
                     /translation="IVAASLPQIQAGVGASADQVSWIQTAYLIPEVVMIPLSGYLSRL
                     WGTQRLYLASCAGFIIMSVLTGLSSSIDMMILTRALQGFIGGAMIPTVFAVAFTAFPP
                     ERRVTASVIMGLIVTLAPTVGPTLGGHLTEWLSWRWLFFINVPIGLVVLFGVARWGDF
                     DKGDPSLRHGFDWFGLAAMAAFLMSLQFVLEEGAKDGWFDDDVILGLSVLALVAGAIF
                     VWRQLSYRNPIVELRAFANRNFSIGLLMTAISGASLFGGTFLLPQFLSRVRNYSASEV
                     GTTMVISGLSMFATGPIAGRLVRRSDPRIPLFVGFMLAGWGMYMAHGVTTEWGFWEFA
                     GVQACRGVGVMIAM"
BASE COUNT          223 a          359 c          329 g          135 t
ORIGIN      
        1 tcattgcgat catgaccccc accccgcgac aagcctgaac cccggcgaat tcccaaaaac
       61 cccactccgt ggtcacgccg tgggccatgt acatgcccca gccggccagc atgaagccca
      121 cgaacaacgg gatgcgcgga tctgacctgc gcaccagccg gccggcgatc ggccccgtgg
      181 cgaacatcga caggcccgaa atgaccatcg tcgtcccgac ctccgacgcg gaatagttcc
      241 tcacccggct caggaactgg ggcagcagga aggtgccgcc aaacaggctg gcgccggata
      301 tggccgtcat caacagcccg atcgagaagt tgcgattggc gaaggcgcgc agctccacga
      361 tcggatttcg gtagctgagt tgccgccaga cgaaaatcgc gccggcgacc agggccagga
      421 ccgacaggcc cagaatgaca tcgtcatcga accagccgtc cttggcgcct tcctccagca
      481 caaattgcag gctcatcagg aaggccgcca tggcggccag gccgaaccag tcgaagccgt
      541 gcctgaggct ggggtcgccc ttgtcgaaat cgccccagcg cgccacgcca aacagcacga
      601 ccaggccgat gggcacgttg atgaagaaca gccaccgcca cgacagccac tcggtcagat
      661 ggccgcccag ggtgggtccg acggtgggcg ccagggtcac gatcaggccc atgatgacgc
      721 tggccgtgac ccggcgctcg ggcgggaagg ccgtgaaggc cacggcgaac acggtcggga
      781 tcatggcgcc gccaatgaag ccctgcagcg cccgcgtcag gatcatcatg tcgatcgacg
      841 aggacagacc cgtcagcacg ctcatgatga tgaagcccgc gcacgaggcc agatacaggc
      901 gctgggtccc ccataggcgc gacagatatc ccgacagggg gatcatcacg acctcgggga
      961 tcagataggc ggtctggatc cagctgacct ggtcggcgct ggcgcccacc ccggcctgga
     1021 tctgcggcag cgaggcggcc acgatc
//
LOCUS       DAOMEO010001288         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14329_length_1045_cov_2.917172, whole genome shotgun sequence.
ACCESSION   DAOMEO010001288 DAOMEO010000000
VERSION     DAOMEO010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14329_length_1045_cov_2.917172"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..>1045)
                     /locus_tag="PLH31_20520"
     CDS             complement(52..>1045)
                     /locus_tag="PLH31_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HQR91611.1"
                     /translation="LDHRIKNLLAVVQSVAAQSARKSASLDVFLKTFAARLKSMSSAH
                     DLLSAARWRGATLARIAAAELGGVAPTQTRWDGPELFLTPRAASALSLTLHELAVNAV
                     KYGALSSENGKVEVVWRRSPEGGFALEWLETGGPPATEPTKRGFGATLIEDVVGRELG
                     GRARMEYKRGGLSALIQASPEALADGPPPEPEKAVPERIVETLIAADENVRPGAIAGL
                     KVLIVEDSLLLALELEAGLEDAGVVVVGCAAELSEALSMLDQVFDVAVLDADLNGQSV
                     APVAEILRREGRPFVFATGYADKAAPMGFDAPIVRKPYNVHQIARAIADVTGRH"
BASE COUNT          163 a          364 c          354 g          164 t
ORIGIN      
        1 tttagagcat ttttcgatcc gataaccgtt tcacacttat cggaaaatgc tctagtgccg
       61 ccccgtcaca tccgcgatgg cccgcgcgat ctggtgcaca ttgtagggct tgcgaacgat
      121 cggcgcgtcg aagcccatgg gcgcggcctt gtcggcatag cccgtggcga acacgaacgg
      181 gcggccttcg cggcgtagaa tttccgccac cggggccacc gactggccgt tgaggtcggc
      241 gtccagcacc gccacgtcga acacctggtc cagcatcgac agggcctcgc tgagctcggc
      301 cgcgcagccg accaccacga ccccggcgtc ttcgagaccg gcttccagct cgagcgccag
      361 caacagagag tcctcgacga tcaacacctt gaggccggct atggccccgg ggcggacgtt
      421 ctcgtcggcg gcgatgaggg tctcgacgat ccgttcgggg acagctttct caggttcggg
      481 cggcgggccg tcggccaggg cctcgggcga ggcctggatc agggcgctga gaccgccgcg
      541 cttgtattcc atgcgagcgc gcccgcccag ttcgcggccc acgacatctt cgatcagggt
      601 ggcgccaaaa ccgcgcttgg tcggctcggt cgccggcggt ccgccggttt ccagccactc
      661 cagcgcaaag ccgccctcgg gcgagcgacg ccagaccacc tcgaccttgc cgttctcgct
      721 cgacagcgcg ccgtacttga ccgcgttgac cgccagttcg tgcagggtca gcgacagggc
      781 cgaggccgcg cgcggggtca gaaacagctc ggggccgtcc catcgcgtct gggtcggcgc
      841 cacgccgccc agctcggcgg cggcgatccg cgccagggta gccccacgcc agcgggcggc
      901 gctcagcaga tcgtgggccg agctcatgga cttcagccgg gcggcgaagg tcttcaggaa
      961 gacgtccagc gaggccgact tgcgggcgga ctgggcggcc accgattgca cgacggccag
     1021 caggttcttg atgcggtggt ccagc
//
LOCUS       DAOMEO010001289         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14339_length_1045_cov_1.520202, whole genome shotgun sequence.
ACCESSION   DAOMEO010001289 DAOMEO010000000
VERSION     DAOMEO010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14339_length_1045_cov_1.520202"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>1045
                     /locus_tag="PLH31_20525"
     CDS             53..>1045
                     /locus_tag="PLH31_20525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR91612.1"
                     /translation="MTQASEAVARSEARYRLLTENALDVIVHSDLKGRVNYISPSVLA
                     VLGYAPEELTGERPVEFVHPDYVDAVMAISRAQVNGQSDRFPDRIEYLAYRKDGALIW
                     LESRPTLAMDPVSGEKIGLTDVVRDITARKVLEQELREARATAEAAAAAKGEFLANMS
                     HELRTPLTAVIGFANLVDDQPELAADTRRHVARIVDGGRSLLATINDILDFSKLDAGH
                     MEIRPRQVDVGHLAREILDIFTLQASAKGLTLASQGLDDLPPNLWLDPERLRQVLLNL
                     VGNAVKFTETGGVTLSLAHDPTSERLAVSVIDTGQGIAEQDLKLLFMRFSQVDAS"
BASE COUNT          185 a          340 c          339 g          181 t
ORIGIN      
        1 cagcctgccc gtagccgcct tccagaggca gaggcgttac attcttgatc gcatgaccca
       61 ggccagcgag gccgtcgcgc gcagcgaagc ccgctaccgt ctgctgaccg agaacgctct
      121 cgacgtcatc gtgcacagcg acctgaaggg tcgggtgaac tatatctccc catcggtcct
      181 tgccgtgctg ggctacgcgc cggaagaact gacgggggag cgtcccgtgg agtttgtcca
      241 tcccgactat gtagatgcgg tgatggcgat cagccgggcc caggtgaacg gtcagagcga
      301 ccgttttccc gatcgcatcg aatatctggc ctaccgcaag gatggcgcgc tgatctggct
      361 ggagtcgcga cccaccctgg cgatggatcc cgtcagcggc gagaagatcg gcctgaccga
      421 cgtggtccgc gacatcaccg cgcgcaaggt gctggagcag gaactgcgcg aggccagggc
      481 cacggctgag gcggcggcgg cggccaaggg cgagttcctg gccaatatga gccatgagct
      541 gcgcacgccg ctgacggcgg tgatcggctt cgccaacctt gtcgacgatc aacccgagct
      601 ggcggctgac acccgtcgcc acgtcgcccg gatcgtcgac ggcggccgtt cgctgctggc
      661 caccatcaat gacattctgg atttctccaa gctggacgcc ggccatatgg aaattcggcc
      721 gcgtcaggtt gatgtcggcc atctggcgcg cgagattctg gacatcttca cgctacaggc
      781 ctcggccaag gggctgaccc tggccagcca gggactggat gacctgccac ccaatctctg
      841 gctcgatccg gagcgcttgc ggcaggtgct gctgaacctt gtgggcaacg ccgtcaaatt
      901 caccgagacc ggcggcgtga ccctgagcct ggcccatgac ccgacgagcg agcgtctcgc
      961 ggtcagtgtc atcgacaccg gccagggcat cgccgagcag gacctcaagc tgctgttcat
     1021 gcggttttcg caggtcgacg cctcg
//
LOCUS       DAOMEO010001290         1043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14372_length_1043_cov_1.780364, whole genome shotgun sequence.
ACCESSION   DAOMEO010001290 DAOMEO010000000
VERSION     DAOMEO010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14372_length_1043_cov_1.780364"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1043
                     /locus_tag="PLH31_20530"
     CDS             <1..>1043
                     /locus_tag="PLH31_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQR91613.1"
                     /translation="TAVLGLADIGPLAAKPVMEGKAVLFKKFAGIDVFDIEINEKHDL
                     DKLVDIIASLEPTFGGINLEDIKAPDCFYIERKLRERMKIPVFHDDQHGTAITVGAAI
                     LNGLKLVGKDISKVKLVTSGAGAAALACLGLLIKLGMPRKNIFVTDLAGVVYEGRTEL
                     MDEDKIQFAQKTSARTLAEVIEGADIFLGLSAGGVLKPAMVQKMAVKPLILALANPNP
                     EITPEDVKSVRDDAIMATGRTDYPNQVNNVLCFPYIFRGALDAGASTITDEMEIAATH
                     ALAELAQAEQSDVVAAAYVGELLAFGPEYLIPKPFDPRLMIKIAPAVAQAAADSGVAL
                     RPIQDMDAYREKL"
BASE COUNT          221 a          312 c          299 g          211 t
ORIGIN      
        1 cacggcagtc ctggggctgg ccgacatcgg cccacttgcc gccaagcctg tgatggaagg
       61 caaggccgtc ctgttcaaga agtttgccgg tatcgatgtc tttgacatcg aaatcaatga
      121 aaagcatgac ctcgacaaac tggtcgacat cattgcctca ctggagccga ctttcggcgg
      181 tatcaacctg gaagacatca aggcacccga ctgcttctac attgaacgca agttgcgtga
      241 gcgcatgaag atcccggtct tccatgacga tcaacacggg accgccatca cggtcggtgc
      301 ggccatcctc aatggcttga agctcgtggg caaggacatc agcaaggtca agctcgtgac
      361 ctccggcgcg ggtgccgccg ccctggcctg cctgggtctg ctgatcaagc ttggcatgcc
      421 ccgcaagaat atctttgtga cggatctcgc aggtgtcgtc tatgagggcc gcaccgagct
      481 catggacgag gacaagatcc agtttgccca gaagacatcg gcccgtacgt tggcagaagt
      541 cattgagggc gccgatattt tcctgggtct ttcggccggc ggtgtgctca agcctgcgat
      601 ggtccagaaa atggctgtca agcccctgat tctggctctg gccaatccca accccgagat
      661 cacgccagaa gacgtcaaat ccgtgcgtga cgatgccatc atggcaactg gccgtaccga
      721 ctatccaaac caggtcaaca acgtcctgtg cttcccctat atctttcgtg gcgcgctgga
      781 tgccggcgca tcgaccatca ccgacgagat ggagattgcg gccacccatg ccctggccga
      841 gctggctcag gccgagcaaa gcgatgtggt ggcggcggcc tatgtcggcg aattgctggc
      901 tttcggaccc gaatacctga tacccaagcc ttttgatccg cgcttgatga tcaaaatcgc
      961 gcctgcggtg gcacaagctg ctgcagacag tggcgtcgcg ctgcgcccga ttcaggacat
     1021 ggatgcctac cgtgaaaagc tgc
//
LOCUS       DAOMEO010001291         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14387_length_1042_cov_1.719352, whole genome shotgun sequence.
ACCESSION   DAOMEO010001291 DAOMEO010000000
VERSION     DAOMEO010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14387_length_1042_cov_1.719352"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..>1042)
                     /locus_tag="PLH31_20535"
     CDS             complement(125..>1042)
                     /locus_tag="PLH31_20535"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQR91614.1"
                     /translation="NFRNTTNAYTGARFDTPVADGALTAFWVMPQVRLPDDRAAIEDN
                     RIAWNRERSDLQFAGLFWTRDLGQRRSLDLYVYGLTESDDADQQTRNLRLATPGVRWR
                     QSPAAGLWDVEVEAAAQVGQVRRSASPADTQDVDVGAGFLHLEAGRTFGHHWSPRLAA
                     QFDYVAGDGGQGDYGRFDTLYGARRFEFGPAGLYGAVSRANLVSPGIRLEVKLNSKLD
                     GFVAVRGLWLEDARDSLGATGVIDPSGDSGRFGGTQIEARVRYKLTPDVTLETGGARL
                     FKGRALTDAPNAPSPDDTTYGYVDVTYAF"
BASE COUNT          177 a          343 c          364 g          158 t
ORIGIN      
        1 cctggtgcag ttggagctgc cggttggtca ccgcccagat gagaaaggcg agggccagca
       61 gcgacacgag gaaactgagc gccagcagga ggccaatggt caccgacatg gggtgaggac
      121 tccgttagaa ggcataggtc acgtccacat agccgtaggt ggtgtcgtcg ggcgacggcg
      181 cgttcggagc gtcggtcagg gcgcggccct tgaagagccg ggcgccgccg gtctccagag
      241 tgacgtcagg ggtcagcttg tagcggaccc gcgcctcgat ctgggttccg ccaaagcggc
      301 cgctgtcgcc cgacgggtcg atgacccccg tcgcgcccag actgtcgcga gcgtcttcca
      361 gccagaggcc ccgcaccgcg acgaagccgt ccagcttgct gttgagtttt acctccagac
      421 gaatgccggg cgacacgagg ttggcgcgtg agaccgcgcc gtagagaccg gcggggccga
      481 actcgaagcg tcgtgcgcca taaagggtgt cgaaccggcc atagtccccc tgtcccccgt
      541 cacccgcgac atagtcgaac tgggccgcca gtcgcggcga ccagtggtgc ccgaacgtgc
      601 ggcctgcctc cagatgaagg aagccggccc cgacatccac atcctgggtg tccgcgggcg
      661 aggcggagcg acggacctgg ccgacctggg ccgccgcctc gacctcaaca tcccatagcc
      721 cggccgccgg cgactggcgc caacggacgc cgggcgtcgc cagccggagg tttcgggtct
      781 gctggtccgc gtcatcgctt tcggtcaggc cgtagacata gaggtcgagc gaccgccgct
      841 ggccgaggtc gcgagtccaa aacaggccgg cgaactgcag gtcggatctc tctcgattcc
      901 acgcgatccg gttgtcctcg attgcggccc tgtcgtccgg caaccgcacc tgcggcatga
      961 cccagaaggc cgtcagggcg ccgtcggcca cgggcgtgtc gaaacgggcg ccggtatagg
     1021 cgttggtggt gttgcgaaaa tt
//
LOCUS       DAOMEO010001292         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14429_length_1040_cov_1.791878, whole genome shotgun sequence.
ACCESSION   DAOMEO010001292 DAOMEO010000000
VERSION     DAOMEO010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14429_length_1040_cov_1.791878"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLH31_20540"
     CDS             <1..244
                     /locus_tag="PLH31_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR91615.1"
                     /translation="TLVAAPLVTGPSAPLVTTVLNGRAGMPAFKDDINDADLAGIITY
                     VRTSWGNKAKPVTAAEIAANRAKAKAAQKARGLQAH"
     gene            269..>652
                     /locus_tag="PLH31_20545"
     CDS             269..>652
                     /locus_tag="PLH31_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HQR91616.1"
                     /translation="MTRFATLATALALASGIAGAASAQEIVRGGNPQSPIASVVTVPA
                     SFDTIYVSGMTPPVVDDKATGVAKYGDTKTQTIGVIKRLQDALKTQGADLGDVVMMRV
                     LLVGDPAKGGKMDFAGMMEGYKMFFG"
     assembly_gap    653..662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <663..762
                     /locus_tag="PLH31_20550"
     CDS             <663..762
                     /locus_tag="PLH31_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91617.1"
                     /translation="QPNKPSRITSQISGLVADGMLVEIEVQAAKKK"
BASE COUNT          184 a          360 c          318 g          168 t
ORIGIN      
        1 caccctggtg gcggcgcctc tggtcacggg tccctcggcg cccctggtga ccacggtcct
       61 gaatggccgg gccggaatgc ctgccttcaa ggacgacatc aacgacgccg atctggccgg
      121 catcatcacc tatgtgcgca cctcctgggg gaacaaggcc aagccggtga cggccgccga
      181 aatcgccgcc aaccgcgcca aggccaaggc cgcccagaag gcgcgcggcc tgcaagctca
      241 ctgatcgacc cttcaaaagg agaagaccat gacccgtttc gccaccctcg ccaccgccct
      301 cgccctggcc tccggtatcg ccggcgccgc ttcggcccag gaaatcgtcc gcggcggcaa
      361 tccgcagtcg ccgatcgcca gcgtcgtcac cgtgccggcc agctttgaca ccatctatgt
      421 cagcggcatg accccgccgg tcgtcgacga caaggccacg ggcgtggcca agtacggcga
      481 caccaagacc cagaccatcg gcgtcatcaa gcgcctgcag gacgcgctca agacgcaggg
      541 cgccgatctg ggcgacgtgg tgatgatgcg ggttctgctg gtcggcgatc cggccaaggg
      601 cggcaagatg gacttcgccg gcatgatgga aggctacaag atgttcttcg gcnnnnnnnn
      661 nnacagccca acaagccctc gcgcatcacc agccagatct ctggcctggt ggccgacggc
      721 atgctggtcg agatcgaagt ccaggccgcc aagaagaagt agcgcgtccg gccccgctgc
      781 ggcgggttcg acccaggatc agggaggccg gcgcctgcgc tggcctccct ttttcatgcc
      841 cgcttgccgg tcggcggcgt gcggcgaata atctttgtga caacgttgtc aaataattcg
      901 agccgggcta tacaggtcgt gcccttccag ggcgtttcct cccaatgact cggccgcctt
      961 ctcctcctgg agggcggccg tctttttgtc cggcccatgc cggccatgat gcttgactca
     1021 agagacggtc cggccgaaac
//
LOCUS       DAOMEO010001293         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14503_length_1036_cov_3.045872, whole genome shotgun sequence.
ACCESSION   DAOMEO010001293 DAOMEO010000000
VERSION     DAOMEO010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14503_length_1036_cov_3.045872"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="PLH31_20555"
     CDS             <1..101
                     /locus_tag="PLH31_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91618.1"
                     /translation="KKIISESGLNVIAANDLSDGAEKIVAAVKGAR"
     gene            102..986
                     /gene="sucD"
                     /locus_tag="PLH31_20560"
     CDS             102..986
                     /gene="sucD"
                     /locus_tag="PLH31_20560"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQR91619.1"
                     /translation="MSILIGSETKVICQGITGAQGTFHSEQAIAYGTKMVGGVTPGKG
                     GQTHIGLPVFDTVGEAKEATGADASVIYVPPPFAADSILEAIDAEIGLIICITEGIPV
                     LDMVKVKKALQGSRSRLIGPNCPGVLTPNQCKIGIMPGGIFSAGSVGVVSRSGTLTYE
                     AVFQTTNAGLGQTTAVGIGGDPVKGTEFIDVLELFLADEATKSIVMIGEIGGSAEEEA
                     AQFLADEAKRGRKKPMVGFIAGRTAPPGRHMGHAGAIISGGKGGAEDKIAAMEAAGIR
                     VSPSPAKLGETLLEVLKG"
BASE COUNT          193 a          358 c          326 g          159 t
ORIGIN      
        1 gcaagaaaat catctcggaa agcggcctga acgtcatcgc cgccaacgat ctgtccgacg
       61 gcgccgaaaa gatcgtcgcc gctgtgaagg gcgcccgcta aatgtcgatc ctcatcggtt
      121 ctgaaaccaa ggtcatctgc cagggcatca ccggcgccca gggcacgttc cactccgagc
      181 aggccatcgc ctacggcacc aagatggtcg gcggcgtcac ccccggcaag ggcggtcaga
      241 cgcacatcgg cctgcccgtg ttcgacacgg tcggcgaagc caaggaagcc acgggcgccg
      301 acgcctcggt gatctacgtg ccgcccccct tcgcggccga ctcgatcctg gaagccatcg
      361 acgccgagat cggcctgatc atctgcatca ccgaaggcat tccggtgctg gacatggtca
      421 aggtcaagaa ggcgctgcag ggctcgcgct cgcgtttgat cggtcccaac tgccccggcg
      481 tcctgacgcc caaccagtgc aagatcggca tcatgccggg cggcatcttc tcggccggca
      541 gcgtcggcgt cgtgtcgcgt tcgggcaccc tgacctatga agcggtgttc cagaccacca
      601 atgccggtct cggccagacc acggccgtcg gcatcggcgg cgacccggtc aagggcaccg
      661 agttcatcga cgtgcttgag ctgttcctcg ccgacgaagc caccaagtcg atcgtcatga
      721 tcggcgaaat cggcggctcg gccgaagagg aagccgccca gttcctggcc gacgaagcca
      781 agcgcggccg caagaagccc atggtcggct tcatcgccgg tcgtacggct cctcccggtc
      841 gtcacatggg ccacgccggc gcgatcatct cgggtggcaa gggcggcgcc gaggacaaga
      901 tcgcggcgat ggaagctgcg ggcatccgcg tctcgccgtc gccggccaag ctgggcgaaa
      961 ccctgcttga ggtcctcaag ggctaaagac ataccgaaac cccgcccgcg cggggcccag
     1021 gttttttgcg accgcc
//
LOCUS       DAOMEO010001294         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14506_length_1036_cov_2.673802, whole genome shotgun sequence.
ACCESSION   DAOMEO010001294 DAOMEO010000000
VERSION     DAOMEO010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14506_length_1036_cov_2.673802"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1036)
                     /locus_tag="PLH31_20565"
     CDS             complement(<1..>1036)
                     /locus_tag="PLH31_20565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR91620.1"
                     /translation="QGRNGEAVLSDVWGLSTRQAALALRLAEGQTRAQAAKATGISEA
                     SAKKQLDVVYATLGVSSAGAMARVVTESRALALLAEATHQDAHAGDEFTEPLGLFARA
                     DGSQVAYSDHGPRAGRPVLVIHSSSASRAIPMRLVAALQARGFRPLALDRPGFGLTDP
                     VVDRDAYRADPFSGACEDVVRLCDHLKLKRLDLIGRGGAQVVLALTRLHPDIAGGVVL
                     VNPDPPTRERAESRGLLTGVKNAFVRHPEGIEALTRLFVTHLSQGHFERMMTRSVADS
                     PPDAAFMRDPRNLADYARGYRLFLTGRIAGYVDEQIAMTRWSSPRRPDAASWRVLMGA
                     HDVLHDPVET"
BASE COUNT          173 a          357 c          349 g          157 t
ORIGIN      
        1 tgggtctcta ctgggtcgtg cagaacgtcg tgggccccca tcagcaccct ccaggacgcg
       61 gcgtccggcc gccgcgggct ggaccagcgc gtcattgcga tctgttcgtc gacatagccc
      121 gcaatccggc cggttaagaa caaccggtag ccccgcgcgt agtcagccag attgcgcgga
      181 tcgcgcatga aggccgcatc cggcgggctg tcggcgacac tgcgggtcat catgcgctcg
      241 aaatggcctt gagacagatg ggttacgaac agccgcgtca gggcctcgat gccctcggga
      301 tggcgaacga aggcgttctt gacgccggtc agcaagcccc ggctctcggc ccgctcgcgg
      361 gtaggcggat caggattgac cagcacgacc cctccggcga tgtctgggtg cagacgggtc
      421 agggccagga ctacttgagc cccgccgcgg ccaatcaggt ccagccgctt cagcttcaag
      481 tgatcgcaca gccggaccac atcctcgcag gcgccgctga acggatcggc gcgataggcg
      541 tcgcggtcaa ccaccggatc ggtgaggcca aatccaggac ggtccagggc cagcggccgg
      601 aacccgcgcg cctgcagggc cgccaccagc cgcatgggaa tggcccgcga ggccgaactg
      661 ctatgaataa ccagcaccgg ccgtcctgcg cgcgggccgt gatcgctgta ggccacctga
      721 gagccgtcgg cacgggcgaa gagacccagg ggttcggtga actcgtcacc ggcatgggcg
      781 tcctggtgcg tcgcctcggc cagcagggcc aaagcgcggc tttcggtaac cacccgagcc
      841 atggcgccgg cgctgctcac gcccaaggtg gcgtacacta catccagctg cttcttggcc
      901 gaggcttcgg agatgcccgt ggccttggcc gcttgagccc gggtctggcc ttcggcgagg
      961 cgaagcgcca gggctgcctg ccgcgtgctc aggccccaca catccgatag caccgcttcg
     1021 ccgtttcgcc cctggg
//
LOCUS       DAOMEO010001295         1034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14549_length_1034_cov_2.768131, whole genome shotgun sequence.
ACCESSION   DAOMEO010001295 DAOMEO010000000
VERSION     DAOMEO010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14549_length_1034_cov_2.768131"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLH31_20570"
     CDS             <1..522
                     /locus_tag="PLH31_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR91621.1"
                     /translation="YGFDQALADLRLQFGVVRALPLFVLGVCLARAVEMDWPSEKAAR
                     VLLYGGVVVLVGWQTLDRYALPDVLWFDLPSLAAIGCIILGAGRLPVKRPRAWIEEGA
                     KLSFALFITHIFVGVIYWSAIHKLIDTVPIGIGWQWLMWLAGFPLAYGGAWLFHTYVD
                     QPVQDRLAPWLRR"
     gene            complement(529..>1034)
                     /locus_tag="PLH31_20575"
     CDS             complement(529..>1034)
                     /locus_tag="PLH31_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR91622.1"
                     /translation="ADQEALIAHLGLTDYDLVGYSLGARTAVRMLTRGARPAKLVLGG
                     MGDSGLMAAGARAAMFEDSIRHGEAAKDPRAGRRVQAMMAASGLVPEAMLGVLTSFVA
                     TSEAQLRALDLPTLVIAGVADDDNGSAEGLAAMMGNARAVRVAGDHLSAVMEPALAAQ
                     IASFLAA"
BASE COUNT          135 a          368 c          354 g          177 t
ORIGIN      
        1 tacggcttcg atcaggccct ggccgatctg cggctccagt tcggcgtcgt gcgggccctg
       61 ccgctgttcg tgctgggcgt gtgcctggcc cgcgcggtcg agatggactg gccctcggaa
      121 aaggcggccc gggtgctgct ctatggcggc gtcgtggtgc tggtcggctg gcagaccctg
      181 gaccgctacg ccctgccgga cgtgctgtgg ttcgacctgc ccagcctcgc cgccatcggc
      241 tgcatcatcc tgggcgcggg ccgcctgccg gtgaagcgtc cccgggcctg gatcgaggag
      301 ggcgccaagt tgtcgttcgc cctgttcatc acccacatct tcgtcggggt gatctactgg
      361 agcgccattc acaagctgat cgacaccgtg ccgatcggca tcggctggca gtggctgatg
      421 tggctggccg gcttcccgct ggcctatggc ggggcatggc tgtttcacac ctatgtggac
      481 cagcccgtgc aggaccggct ggcgccgtgg ttgaggcggt agcccgtttc aggccgccag
      541 aaagctcgcg atctgcgccg ccagggccgg ttccatcacc gcgctgaggt gatccccggc
      601 cacgcgcact gcccgcgcat tgcccatcat cgccgcaaga ccctcggccg agccattgtc
      661 gtcgtcggcg acgccggcga tcaccagggt cggtaggtca agggcgcgca gctgcgcctc
      721 ggacgtggcc acaaacgagg tcagcacgcc cagcatcgcc tcggggacga ggccgctggc
      781 cgccatcatc gcctgcaccc gccggcctgc acgcgggtct ttggcggcct cgccgtggcg
      841 gatagagtcc tcgaacatcg ccgcgcgtgc gccggccgcc atcaggccgc tgtcgcccat
      901 gccgccgagc accagtttcg ccggccgggc gccgcgggtc agcattctca ctgccgtccg
      961 cgcgcccagc gaatagccga cgagatcata atcggtcaga cccagatggg cgatcagcgc
     1021 ctcctggtcg gcgg
//
LOCUS       DAOMEO010001296         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14575_length_1033_cov_2.320041, whole genome shotgun sequence.
ACCESSION   DAOMEO010001296 DAOMEO010000000
VERSION     DAOMEO010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14575_length_1033_cov_2.320041"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLH31_20580"
     CDS             complement(<1..1032)
                     /locus_tag="PLH31_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HQR91623.1"
                     /translation="MTAAALSKVLKGVEITLIESDQIATVGVGEATIPPINTFNQILG
                     IDEADFMRSTKASFKLAIEFVDWTAPGSRYLHPFGGFGLDIEAIKFHQFWLKAQSQGS
                     TQPLDDFNLSATAAKLGKFMLPSKDPGQVLSSLKYAFHFDAVLYAGYLRDYAEKRGVI
                     RREGKVVDVCLRGEDGFIASVTTDGGEVIEADLFIDCSGFRGLLIEQALDTGFEDWGH
                     WLPNDRAVAMPCDTGGDGLTPYTRATADKAGWRWRIPLQHRTGNGYVYSSQHLSDEDA
                     VARLRATLDGPARAEPNFIKFRAGRRKQIWVKNCVAIGLASGFLEPLESTSIHMIQAG
                     ITKLLALFPD"
BASE COUNT          166 a          323 c          361 g          183 t
ORIGIN      
        1 gtccgggaac agggccagca gcttggtgat gccggcctgg atcatgtgga tgctggtgct
       61 ttccaggggc tccaggaagc ccgaggccag gccgatggcg acgcagttct tgacccagat
      121 ctgcttgcgg cgaccggccc ggaacttgat gaaattgggc tcggcgcggg ccgggccgtc
      181 cagggtggcg cgcaggcggg cgacggcgtc ctcgtcgctg aggtgctggc tggaatagac
      241 atagccgttg ccggtgcggt gctgcagcgg gatgcgccag cgccagccgg ccttatcggc
      301 cgtggcccgg gtatagggcg tcaggccatc gccgccggtg tcgcacggca tggccacggc
      361 gcggtcattg ggcagccagt ggccccagtc ctcgaagccg gtgtccagcg cctgctcgat
      421 cagcaggccc cgaaagcccg agcagtcgat gaacaggtca gcttcgatca cctcgccgcc
      481 gtcggtggtg accgaggcga tgaagccgtc ctcgccgcgc aggcagacgt ccaccacctt
      541 gccctcgcgc cggatcacgc cgcgcttttc ggcatagtcg cgcagatagc cggcatagag
      601 gacggcgtca aaatggaagg cgtatttcag gctcgacagc acctggccgg ggtccttgct
      661 gggcagcatg aacttgccaa gcttcgccgc cgtggccgac aggttgaagt cgtcgagggg
      721 ttgggtggat ccctggcttt gcgccttcag ccagaactgg tggaacttga tggcctcgat
      781 atccaggccg aaaccgccga acgggtgcag gtagcgtgag ccgggggctg tccagtcgac
      841 gaactcgatg gccagcttga agctggcctt ggtcgaccgc atgaagtcgg cctcgtcgat
      901 gcccaggatc tggttgaagg tgttgatcgg cgggatcgtc gcctcgccca ccccgacggt
      961 ggcgatctgg tcgctctcga tcagcgtgat ctcgacacct ttcagcacct tggacagcgc
     1021 cgccgccgtc atc
//
LOCUS       DAOMEO010001297         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14576_length_1033_cov_2.304703, whole genome shotgun sequence.
ACCESSION   DAOMEO010001297 DAOMEO010000000
VERSION     DAOMEO010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14576_length_1033_cov_2.304703"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLH31_20585"
     CDS             <1..446
                     /locus_tag="PLH31_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HQR91624.1"
                     /translation="AFNAVGIIGASQINRLLLRRTTPDHVLMRASLASLVFAFLLVGA
                     AWTGWGGQWSVLPLLFLSLSTYGLMGGNTMAGALSVDPKRAGSTSALMGSASFGAGAI
                     ASWVSGLLHDGSPRPMAGVMFICLVGSALAIFLIALPKGAAKPDA"
     gene            complement(443..823)
                     /locus_tag="PLH31_20590"
     CDS             complement(443..823)
                     /locus_tag="PLH31_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HQR91625.1"
                     /translation="MAIWTPRTWTGLAALSGLCAVALGAFAAHGIDAPKPAEWLRTGS
                     LYQMTHALAVLGAFALHRTGARGMGLAAGLFLGGTLLFSGSLYAMALGGPRLLGAITP
                     LGGLAFMIGWALLAWRAFTLPATD"
     gene            936..>1033
                     /locus_tag="PLH31_20595"
     CDS             936..>1033
                     /locus_tag="PLH31_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91626.1"
                     /translation="MALTWGDDYPIHQTPEPVAYAGTDRNFYDRYF"
BASE COUNT          148 a          350 c          359 g          176 t
ORIGIN      
        1 tcgccttcaa cgccgtcggc atcatcggcg ccagccagat caaccggctg ctgctgcgcc
       61 ggaccacgcc cgaccacgtg ttgatgcgcg ccagcctcgc ttcgctggtc tttgccttcc
      121 tgctggtcgg agcggcctgg accggctggg gcgggcagtg gagcgtgctg ccgttgctgt
      181 tcctgtcgct gtcgacctac ggcctgatgg ggggcaacac catggcgggg gcgctgagcg
      241 ttgatcccaa gcgtgcaggc tcgacctcgg ccctgatggg ctcggcctcg tttggcgccg
      301 gggccatcgc gtcctgggtc agcggcctgc tgcatgacgg ctcgccgcgc ccgatggccg
      361 gcgtgatgtt catctgcctg gtgggatcgg ccttggcgat cttcctgatc gctttgccca
      421 agggcgccgc caagcctgac gcctagtcgg ttgcgggcag ggtgaaggcg cgccaggcca
      481 gcagggccca tccgatcatg aaggccaggc cgcccagcgg tgtgatcgcg cccaacagac
      541 gcggcccgcc cagggccatg gcgtagagcg atccggagaa cagcagggtt ccgcccagga
      601 acagcccggc ggccagcccc atgccccgcg cgccggtccg gtgcagggcg aaggcgccca
      661 gcaccgccag ggcgtgggtc atctgatata gcgagccggt gcgcagccat tcagccggct
      721 tgggcgcgtc gatcccgtgg gcggcaaagg cgcccagggc cacggcgcac agtccgctca
      781 gcgcagccag gcctgtccag gtcctgggag tccagatggc catggggttt acgctccgtc
      841 acggttgcaa tcgttcggcg cgaccttaac gtcgttcatc tgcccgtaaa gccgccccgt
      901 cagagggcca ggcgcgggca gtttgggagg acggcatggc gctcacctgg ggtgacgatt
      961 acccgatcca tcagacgccg gagcctgtgg cctatgccgg cacggatcgc aatttctatg
     1021 accgctattt ctt
//
LOCUS       DAOMEO010001298         1033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14579_length_1033_cov_2.065440, whole genome shotgun sequence.
ACCESSION   DAOMEO010001298 DAOMEO010000000
VERSION     DAOMEO010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14579_length_1033_cov_2.065440"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLH31_20600"
     CDS             <1..466
                     /locus_tag="PLH31_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR91627.1"
                     /translation="VLVDSSIQPPEKRWKGPPRGEGFGSRVYPTLVEALARFRLAPPQ
                     TCENLYIADYIARRSLKEVEGGWTWKFDPAIWQRLSMPDLGELLTRIACPAALMWGDR
                     SNLMHAETLGYMIDQMPKDVLLVPIPDADHHVMIDQPLAFVAGLRGLLANWR"
     gene            463..>1033
                     /locus_tag="PLH31_20605"
     CDS             463..>1033
                     /locus_tag="PLH31_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HQR91628.1"
                     /translation="MTRPGGESSNMVMVYMPDRGVGDLMWHLPTLRAIAARALEGKVL
                     LAARPSSRARELLAGEPCVEAVDYLEYRSGAFRHLVEILDFYRLCRRRRPRAVWILEK
                     IDRPAIGAALAGVRERRGFGLGHGQERWLTHGPFLPRAMRPAHRIEKLAAFEEAHGLK
                     VASREPALVPAPAALEVVEHCFGDRPKPWI"
BASE COUNT          149 a          352 c          353 g          179 t
ORIGIN      
        1 cgtcctggtc gattccagca tccagccgcc cgagaagcgt tggaaggggc cgccgcgtgg
       61 cgaggggttt ggctccaggg tctatccgac gctcgtcgag gcattggccc gcttccgtct
      121 ggccccgccc cagacctgcg agaacctcta tatcgccgac tacatcgcgc gccgctctct
      181 gaaggaagtt gaaggcggct ggacctggaa attcgatccg gccatctggc agaggctctc
      241 gatgccggat ttgggtgaac tgcttacccg tatcgcctgc cccgccgccc tgatgtgggg
      301 ggatcggtcg aaccttatgc acgccgaaac cctgggctat atgatcgatc agatgcccaa
      361 ggacgttctg ctcgtgccga tccccgacgc cgaccaccat gtgatgatcg accagcccct
      421 ggcctttgtg gccgggctgc gcggcctgct cgccaactgg cgatgaccag gccggggggc
      481 gagtcttcga acatggtgat ggtctacatg cccgaccggg gcgtgggcga cctgatgtgg
      541 catctgccca ccctgagggc catcgccgcg cgagcgctgg aaggaaaggt gcttctcgcc
      601 gcccgcccgt ccagccgggc gcgcgagctg ctggccggcg agccgtgcgt cgaggccgtc
      661 gactatctcg agtaccgttc cggcgcgttt cgccatctgg tcgagatcct tgatttctac
      721 cgcctgtgcc gacggcgccg gccgcgcgcg gtctggatcc tggaaaagat cgaccgcccg
      781 gccatcggcg cggccctggc cggcgtgcgc gagcggcggg gctttggtct gggtcacggt
      841 caggagcgct ggctgaccca tggacccttc ctgccgcgcg ccatgcggcc cgcgcaccgg
      901 atcgagaaac tggcggcctt cgaggaagcg cacggcctga aggtggccag ccgcgagccg
      961 gcgctcgtgc ccgcgccagc cgcgctggag gttgtcgagc actgcttcgg cgaccggccc
     1021 aagccgtgga tcg
//
LOCUS       DAOMEO010001299         1032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14617_length_1032_cov_1.602866, whole genome shotgun sequence.
ACCESSION   DAOMEO010001299 DAOMEO010000000
VERSION     DAOMEO010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14617_length_1032_cov_1.602866"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLH31_20610"
     CDS             complement(<1..236)
                     /locus_tag="PLH31_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQR91629.1"
                     /translation="MSDVHTINRSAERIGGGIGGGLPLLALPLLIGAGIWAFTSADAH
                     GAGYNALGAVLITLAALISGGFYSMQPNEAYVLTL"
     gene            364..>1032
                     /locus_tag="PLH31_20615"
     CDS             364..>1032
                     /locus_tag="PLH31_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR91630.1"
                     /translation="MARYLFTTWEGGGHVQPLLLAARELKDRGHTIMILSDACNAADA
                     AALALPFQPWTTAPSQTGKQREDDRLKDHEADNPLEVIQRLIDRVMAGPALAYARDTL
                     AMIDTFKPDAIVSQELLLGVMAAAEKRALPMALFAANIWSLPTLPDVPPFGAGMPPAA
                     SDEDRAMHAMVTQMSRGLFQIGLPALNAARTELGLSPLDDLFAQLDGARSILIATSRA
                     FDFAP"
BASE COUNT          187 a          328 c          324 g          193 t
ORIGIN      
        1 agcgtcagga cataggcttc gttgggttgc atcgaataga agccgccgct gatcagagcc
       61 gccagggtga tcagcacggc gccaagcgca ttgtagcccg caccatgggc atcagcactt
      121 gtgaaggccc agatgcccgc tccgatcagg agcggtagtg cgagaagcgg taggcctcca
      181 ccgataccgc cgccaatacg ctctgctgac cggttgatgg tgtgaacgtc tgacatcgca
      241 gcccccatga gatatgaaaa tgatatcatt ttgatcgctc ttggcaagat gaagcgggac
      301 cgtgtaggcc cgtttgcctg agcggtgggg cccgagcgat ggggcctgag cgatggggat
      361 ggaatggcgc gttatctttt caccacctgg gagggcggcg gacatgtgca gcccctgttg
      421 ctggctgcgc gcgagctgaa ggatcgcggt catacgatca tgatcctcag cgacgcgtgc
      481 aacgcggcgg acgcggccgc cctggccctg cccttccagc cctggacgac cgcaccgagc
      541 cagactggga agcagcgcga ggacgatcgc ctgaaggacc atgaggccga caatcccctg
      601 gaggtgattc aacggcttat cgatcgcgtc atggccggtc cggccctggc ctatgcgcgg
      661 gatacgctcg ccatgatcga caccttcaag ccagacgcta tcgtcagcca ggagctcttg
      721 ttgggtgtca tggcggccgc cgaaaagcgg gccctgccca tggccttgtt cgccgccaat
      781 atctggtcgc ttccgacctt gcccgacgtt ccccctttcg gcgcaggcat gccccccgcc
      841 gccagtgacg aagatcgcgc catgcacgcc atggtcaccc agatgagtcg ggggcttttt
      901 cagatcggcc tgccagccct gaacgctgcc cgaaccgaac ttggcctatc gccgctggat
      961 gatctgttcg cgcagctcga tggggcgcgt tcgatcctga tcgccaccag ccgcgcgttc
     1021 gatttcgcgc ct
//
LOCUS       DAOMEO010001300         1031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14625_length_1031_cov_2.413934, whole genome shotgun sequence.
ACCESSION   DAOMEO010001300 DAOMEO010000000
VERSION     DAOMEO010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14625_length_1031_cov_2.413934"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>368
                     /locus_tag="PLH31_20620"
     CDS             <1..>368
                     /locus_tag="PLH31_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012279482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HQR91631.1"
                     /translation="MAMDAVEDEDGNVTMVPVMVDGAPVVSQAALRLRDELVTHLACL
                     PAVPGVLDAVLEALGPEQVAEITGRSRRVIHRDGRRVVERRSASAAKAETDAFMSGKK
                     RVLVFSDAGGTGRSYHADLG"
     assembly_gap    369..378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <379..>1031
                     /locus_tag="PLH31_20625"
     CDS             <379..>1031
                     /locus_tag="PLH31_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HQR91632.1"
                     /translation="RSYHASLDARNQQRRVHYLVEPGWRADAAIQGLGRSHRTNQAVA
                     PLFRPVTTDIHGEKRFLSTIARRLDSLGALTRGERRTAGNGLFRAEDNLESPWAHRAL
                     QAFYVALHFGNVEAMDRETFEAKTGLRLMDGDGELRASDDMPAMNTFLNRLLALRIAD
                     QNSLFSAFDEILTSILERAAASGALDRGMEDIVADDLSVLDEEVIRTDAVTGAETRL"
BASE COUNT          163 a          333 c          368 g          157 t
ORIGIN      
        1 catggcgatg gacgcggtcg aggacgagga cggtaacgtc accatggtcc cggtgatggt
       61 cgatggcgcg cctgtcgtca gccaggcggc tctgcgactg cgcgacgaac tcgtgaccca
      121 tctggcctgc ctgccggcag tgccgggcgt gctggacgcc gtgctcgagg cgctgggtcc
      181 tgagcaggtc gctgagatca ccgggcgcag ccgacgggtg atccaccgcg atggccgccg
      241 cgtggtcgag cggcgcagcg cctcggcggc caaggccgag accgacgcct tcatgagcgg
      301 caagaagcgc gtgttggtgt tctccgacgc gggcgggacc gggcgcagct atcacgctga
      361 tctgggcgnn nnnnnnnngg cggagttatc atgccagcct tgatgccagg aaccagcagc
      421 gccgggtcca ttatctggtc gagccgggct ggcgagccga tgcggcgatc cagggcctgg
      481 gccgttcgca ccgcaccaac caggccgttg cgccgctgtt tcgaccggtg accacggaca
      541 tccacggcga gaaacggttt ttgtcgacca ttgcgcggcg gctcgacagc ctgggcgccc
      601 tgacccgggg cgagcgccgg acagcgggca acggcctgtt ccgggccgag gacaatctcg
      661 aaagcccttg ggcgcaccgc gcgctgcagg ccttctacgt cgcgctgcac ttcggcaacg
      721 tcgaggcgat ggaccgggag accttcgagg ccaagaccgg cctgcgcttg atggacggcg
      781 acggcgagct gcgggcgtct gacgacatgc cggcgatgaa caccttcctc aatcggttgc
      841 tcgcccttcg catcgccgat cagaacagcc tgttctcggc gttcgacgag atcctgacca
      901 gcatcctaga gcgcgcggcg gcctccggag cgctcgaccg ggggatggag gacatcgtcg
      961 ccgacgacct gtcggtgctc gacgaagagg tcatccgcac ggatgcggtg accggggcgg
     1021 aaacccgtct g
//
LOCUS       DAOMEO010001301         1031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14640_length_1031_cov_1.719262, whole genome shotgun sequence.
ACCESSION   DAOMEO010001301 DAOMEO010000000
VERSION     DAOMEO010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_1031_cov_1.719262"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /gene="mdh"
                     /locus_tag="PLH31_20630"
     CDS             complement(<1..892)
                     /gene="mdh"
                     /locus_tag="PLH31_20630"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQR91633.1"
                     /translation="MARAKIALIGAGMIGGTLAHIAAREELGDIVLFDISEGTPQGKA
                     LDLAEASAVFGKDIALKGANDYADIAGADVVIVTAGVPRKPGMSRDDLLGINLKVMKA
                     VGEGIKQHAPNAFVICITNPLDAMVWALQQFSGLPKEKVIGMAGVLDSARFAYFLAEK
                     TGVSVEDIHAWTLGGHGDDMVPMVRHSTVGGLPLPELVKQGWMTQEELDGIVKRTRGG
                     GGEIVALLKTGSAFYAPAESAIAMATSYLKDKKRVLPCATFLTGQYGLKDLYVGVPVV
                     IGAGGAERVIEFETNDEEKAM"
BASE COUNT          169 a          334 c          338 g          190 t
ORIGIN      
        1 acatcgcctt ttcctcgtcg ttggtttcga actcgatgac ccgttccgcg ccgccagcgc
       61 cgatcacgac aggcacgccg acatacagat ccttcaggcc gtactggccg gtcaggaagg
      121 tggcgcacgg caggacgcgc ttcttgtcct tcagatagct ggtggccatt gcgatggccg
      181 attcagccgg agcgtagaag gccgagccgg tcttcagcag ggccacgatc tcgccgccgc
      241 cgccgcgggt gcgcttgacg atgccgtcga gctcttcctg ggtcatccag ccctgcttga
      301 ccagttccgg cagcggcagt ccgccgaccg tcgagtgacg gaccatcggc accatgtcgt
      361 cgccgtggcc gcccagcgtc caggcgtgga tgtcttcgac cgacacgccg gtcttctcgg
      421 ccaggaaata ggcgaagcgc gccgagtcca ggacaccggc catgccgatg accttttcct
      481 tgggcaggcc cgagaactgc tgcagggccc agaccatcgc gtcgagcggg ttggtgatgc
      541 agatgacgaa ggcgttgggg gcgtgctgct tgatgccttc gccgacggcc ttcatgacct
      601 tcaggttgat gcccagcagg tcgtcgcggc tcatgcccgg cttgcgcggc acgccggcgg
      661 tgacgatcac gacgtcggcg ccggcgatgt cggcatagtc attggcgccc ttcagggcga
      721 tgtccttgcc gaacaccgcc gaggcttcgg ccaggtcgag ggccttgccc tggggcgtgc
      781 cttcgctgat gtcgaacagg acgatgtcgc ccagctcttc gcgagccgcg atgtgagcca
      841 gggtgccgcc gatcatgccg gcgccgataa gggcgatctt cgcgcgagcc atagggtctc
      901 cgtcaggact gtggatggaa attcttgcgg cggtctagac ccatgaggcc cgagcctcaa
      961 gcccccttcc ccgtttcgct gcagtgcggc aaaacagtct gaacaacgat aaggatgcgc
     1021 agggatgagc a
//
LOCUS       DAOMEO010001302         1031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14643_length_1031_cov_1.598361, whole genome shotgun sequence.
ACCESSION   DAOMEO010001302 DAOMEO010000000
VERSION     DAOMEO010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14643_length_1031_cov_1.598361"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..335
                     /locus_tag="PLH31_20635"
                     /pseudo
     CDS             1..335
                     /locus_tag="PLH31_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519232.3"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
     assembly_gap    336..345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <346..491
                     /locus_tag="PLH31_20640"
     CDS             <346..491
                     /locus_tag="PLH31_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR91634.1"
                     /translation="TEVWGYNAGVTTHTLETHVYRLRQKIEPDPAMAKILITEMGGYR
                     LQP"
     gene            complement(573..>1031)
                     /gene="secB"
                     /locus_tag="PLH31_20645"
     CDS             complement(573..>1031)
                     /gene="secB"
                     /locus_tag="PLH31_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQR91635.1"
                     /translation="DDNAAAQSGIRIMAQFIRDFSFESPHAPDSLRSGTAQPAIDMGV
                     EMNARGRTDGLFEVDLKLSARATREDQPVFHVEVVYGGLFHISGVPEAALEEVLLIEC
                     PRFLFPFARRVIADITSEGGFPPFLIDPIDFAGVYAARKAQAEGVVVGNA"
BASE COUNT          196 a          332 c          329 g          164 t
ORIGIN      
        1 cggccgcgag gcctgccggc tgatgcgcaa gaacggtgtg atggccccgg tgatcatgct
       61 gacggcggcc gccagcgaca gcgacaccat cctgggcctg gaatccggcg ccaacgacta
      121 tgtcaccaag ccctttcgct ttggcgtgct tctggcccgc atccgcgccc agctgcgcag
      181 ctacgaggct tcggaagacg cgcttttccg catcggtccc tacgagttcc gcccttcggc
      241 caagcttctg gtcgacgaga agcagaggaa ggtgcggttg accgagaagg aaaccaatat
      301 cctgaaatac ctctaccgcg ctggctcaaa gcctgnnnnn nnnnntgacc gaggtctggg
      361 gctacaacgc cggggtgacg acccacaccc tggaaaccca cgtctatcgc ctgcgccaga
      421 agatcgagcc cgatccggcc atggccaaga tcctgatcac cgagatgggc ggctatcggc
      481 ttcagcctta gcgacggacc gcgatagaaa aagaaaaacc ccggccgcat cgctgcgccg
      541 gggtttctca attctggcgg gtcgaaaacg cctcaggcgt tgccgaccac cacgccttcg
      601 gcctgggcct tgcgggcggc atagacgccg gcgaagtcga tcgggtcgat caggaagggc
      661 gggaagccgc cttcgctggt gatgtcggcg atcacccggc gggcgaaggg gaacaggaag
      721 cgcgggcact cgatcagcag cacttcctca agcgccgctt ccggcacgcc ggaaatgtgg
      781 aacaggccgc catagaccac ttccacatgg aacaccggct gatcttcgcg cgtggcgcgg
      841 gccgacagct tcaggtccac ctcgaacagg ccgtcggtgc gaccgcgggc gttcatctcg
      901 acgcccatgt cgatggccgg ctgggccgtg cccgaacgca ggctgtcggg cgcgtgcggg
      961 ctctcgaacg agaaatcccg gatgaactgg gccatgatgc ggatgcccga ttgagcggct
     1021 gcattgtcgt c
//
LOCUS       DAOMEO010001303         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14707_length_1028_cov_2.132580, whole genome shotgun sequence.
ACCESSION   DAOMEO010001303 DAOMEO010000000
VERSION     DAOMEO010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14707_length_1028_cov_2.132580"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLH31_20650"
     CDS             complement(<1..738)
                     /locus_tag="PLH31_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR91636.1"
                     /translation="MSDKRRPWAQDLLWRGEALGFDLFIALFRLLGVDAASSLGGWIG
                     RTFGPLSGAHKVAERNLKLAFPEKDAAWRAETLVAQWDGLGRSFAEFPLMDKILPSTG
                     RVEVVGKERLTEIAEKKIPVVFVSGHLSNWEVMPAAIVDSGVICEMTYRAANNPYVDK
                     RIKDSRFRYGVRLFAPKGGDGARELLEGMKEGRSVALMNDQKFNNGVAAPFFGHDART
                     APGPTRLAIRFGTVLQPMSVQRIKGARF"
     gene            857..>1028
                     /locus_tag="PLH31_20655"
     CDS             857..>1028
                     /locus_tag="PLH31_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91637.1"
                     /translation="MASMGNDIDVHLGKRLRRRRRLLGLTQQQLAGTVGVRFQQIQKY
                     ECGANRISAARLW"
BASE COUNT          183 a          361 c          308 g          176 t
ORIGIN      
        1 gaaccgcgcg cccttgatcc gctgcacgga catcggctga aggaccgtgc caaaacggat
       61 cgccagccgg gtcggcccgg gcgcggtgcg cgcgtcatgg ccaaagaacg gggccgccac
      121 gccgttgttg aacttctggt cgttcatcag ggcgaccgac cggccttcct tcatgccttc
      181 cagcaactcc cgcgcgccat ctcccccctt gggcgcaaac agccgcacgc cataacggaa
      241 gcggctgtcc ttgatccgct tgtcgacata ggggttgttg gccgcgcgat aggtcatctc
      301 gcagatcacc ccgctgtcga cgatggcggc cggcatcact tcccagttcg acaggtggcc
      361 agaaacgaac accaccggaa tcttcttctc ggcgatctcg gtcaggcgct ccttgcccac
      421 cacctcgacc cggcctgtgg acggcaggat cttgtccatc agcgggaact cggcgaatga
      481 gcgcccaagc ccgtcccatt gcgcaacgag cgtctctgcg cgccaggcgg cgtccttttc
      541 cgggaaagcc agcttcagat tgcgctccgc aaccttgtgc gcgccgctga gcgggccaaa
      601 cgtgcgaccg atccagccac cgaggctgga cgccgcatcc acgcccagca ggcggaacag
      661 ggcgataaag aggtcaaagc ccagcgcctc accgcgccag agcagatcct gggcccaagg
      721 ccgacgctta tccgacatgc cttcttagta ggctggcttg cacgctgcgc gcaacgccac
      781 ttcgcccggc acgcgacttg cggccaacaa gcacgaccgt tccgtttcgg gacgctcgat
      841 actctagcgg gggggcttgg cttccatggg taacgacatc gacgtgcatc tgggcaagcg
      901 tctgcgccgt cgtcgccgtc ttctgggtct gacccagcag caactggccg gcacggtggg
      961 cgttcgattc cagcagatcc aaaagtacga gtgcggcgcc aatcgcattt cggccgctcg
     1021 cctctggc
//
LOCUS       DAOMEO010001304         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14796_length_1023_cov_3.788223, whole genome shotgun sequence.
ACCESSION   DAOMEO010001304 DAOMEO010000000
VERSION     DAOMEO010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14796_length_1023_cov_3.788223"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PLH31_20660"
     CDS             <1..>1023
                     /locus_tag="PLH31_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR91638.1"
                     /translation="LVQHRDDWIDNAFTGKKDALGGFDEKAARLQVLFTPTEALSALF
                     NVHARDLDGTSATFRANVLTAGSNKLNANYDRNTVYFDAGGGNPQKYKGWGTSANIGY
                     DFGDVTLTSITAYEETHGSSRGDIDGGNLTGPGFIPFPSESTDGIKKLDQVTQEIRLA
                     SDTDGPLSWQVGGFYFDTSYQIRTDPGFAPATTLEQENTSWAVFGQASYKATEALTLT
                     GGLRYTSDDKDLRVVSGPNPVAPVSVSDEKVSWDLSAFYSVAENVSVYGKVASGFRGP
                     SIQGRDIAFFSPASVAQSETIMSYEIGLKSELMDRRVRLNGALFTYTIDDPQFSAVGG
                     GSNSNRL"
BASE COUNT          201 a          354 c          296 g          172 t
ORIGIN      
        1 ctggtccagc atcgcgacga ctggatcgac aacgccttca ccggcaagaa ggacgccctc
       61 ggcggctttg acgaaaaggc cgcgcgcctg caggtgctgt tcacgccgac cgaagcgctc
      121 agcgccctgt tcaacgtcca tgcccgtgat ctggacggca cttcggcgac cttccgcgcc
      181 aacgtgctga ccgcaggatc caacaagctc aacgccaatt acgatcgcaa taccgtctat
      241 ttcgacgccg gtggcggcaa cccgcaaaag tacaagggct ggggcacttc ggccaatatc
      301 ggctacgact ttggcgacgt taccctgacc tcgatcacgg cttacgaaga gacccacggc
      361 tccagccgcg gcgacatcga cggtggcaat ctgaccggtc cgggcttcat tcccttcccg
      421 tcggaaagca ccgacggcat caagaagctg gaccaggtca cccaggaaat ccgcctggcg
      481 agcgacactg atggccctct gagctggcag gtcggcggct tctatttcga caccagctac
      541 cagatccgca ccgacccggg ctttgcgccg gcgaccacgc ttgagcagga aaacacctcc
      601 tgggccgtgt ttggtcaggc ctcctacaag gcgaccgaag ccctgaccct gacgggcggc
      661 ctgcgctaca ccagcgacga caaggacctg cgcgtcgtct cgggccccaa cccggtcgcg
      721 ccggtctcgg tctctgacga aaaggtaagc tgggacctca gcgcgttcta tagcgtcgcc
      781 gagaatgtca gcgtctacgg caaggtcgcc tcaggcttcc ggggcccgtc gatccagggc
      841 cgcgacatcg ccttcttctc gccggcctcg gtcgcccagt ccgaaacgat catgtcgtac
      901 gaaatcggcc tgaagagcga actgatggat cgccgcgttc gcctgaacgg cgcgctgttc
      961 acctacacga tcgatgatcc gcaattcagc gcggtgggcg gcggctccaa ctccaaccgc
     1021 ctg
//
LOCUS       DAOMEO010001305         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14813_length_1023_cov_1.763430, whole genome shotgun sequence.
ACCESSION   DAOMEO010001305 DAOMEO010000000
VERSION     DAOMEO010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14813_length_1023_cov_1.763430"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PLH31_20665"
     CDS             <1..>1023
                     /locus_tag="PLH31_20665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQR91639.1"
                     /translation="ADVVKIEKLPGGDDSRNYRDPLVNGVSAPFMIMNRNKRGIAVNL
                     KLEEGRAVLLRMVAQADVLIENFRGGTLDKLGLGYEVLSKANPGLIYCAITGYGRNGP
                     YASKGGFDLIAQGFAGLMSVTGEPGRPPVKTGNPVSDMNAGILAALGITSAYVHKLKT
                     GQGQIVDTSLMDAALQQTYWHAAVFFATGNSPGPTGSAHLLTAPYQAFQTSDGWINIG
                     GANQSNWERITDVLGHPEWREDPRYATNSDRMANLDSLTQAMNAVLVTRTRAEWSAAL
                     DAAGVPAGPVHTIGEALSHPQTLARGMVLDLVHPEAGPTKAIGCPIHFSATPTQVTRP
                     APLLGE"
BASE COUNT          188 a          354 c          320 g          161 t
ORIGIN      
        1 gtgccgatgt tgtgaagatc gagaagctgc ccggcggcga cgactcgcgc aactaccgcg
       61 acccgctcgt caacggcgtg tcggcgccct tcatgatcat gaaccgcaac aagcgtggca
      121 tcgcggtcaa cctcaagctc gaggaaggcc gagctgtgct gctgcgcatg gtggcacagg
      181 ccgatgtgct gatagagaac ttccgtggcg gcacgctgga caagctgggc ctcggctatg
      241 aggtgctgtc caaggccaac ccgggcctga tctactgcgc catcacaggt tatggacgca
      301 acggccccta tgccagcaaa ggggggttcg atctgattgc gcagggcttt gccggcctga
      361 tgtccgtgac gggtgagccc ggcaggccgc cggtgaaaac cggcaacccg gtctcagaca
      421 tgaatgccgg catcctggcc gcgctcggca tcacctcggc ctatgtgcac aagctcaaga
      481 ccggtcaagg ccagatcgtc gacacctcgc tgatggatgc cgccctgcag cagacctact
      541 ggcatgcggc ggtctttttc gcgacgggca attcgcctgg gccgaccggc tcggcccact
      601 tgctgacggc gccctatcag gccttccaga ccagcgacgg ctggatcaac atcggcggcg
      661 ccaaccagtc caactgggag cgcatcaccg acgtgctggg ccatcccgag tggcgcgagg
      721 acccacgcta tgccaccaac tcggatcgca tggccaacct cgacagtctg acccaggcca
      781 tgaatgccgt gctcgtcacc cggacgcggg ccgagtggag cgccgcgctg gacgcagctg
      841 gtgtaccagc cggtccggtg cacaccatcg gcgaggcctt gagccacccg caaaccctgg
      901 cgcgcggcat ggtgctggat ctggtccacc ccgaagccgg cccgaccaag gccatcggct
      961 gcccaatcca tttctcggcc acgcccacac aggtcacgcg ccctgccccg ctgctgggcg
     1021 aac
//
LOCUS       DAOMEO010001306         1022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14837_length_1022_cov_2.089969, whole genome shotgun sequence.
ACCESSION   DAOMEO010001306 DAOMEO010000000
VERSION     DAOMEO010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14837_length_1022_cov_2.089969"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..505
                     /gene="yajC"
                     /locus_tag="PLH31_20670"
     CDS             197..505
                     /gene="yajC"
                     /locus_tag="PLH31_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQR91640.1"
                     /translation="MPGGLSAQLMQIAPILLMVVLFYFMLIRPQQKRAKEHQIMLSNL
                     KRGDNVVLSSGMLGKIVRVEDKEVGLEIAQGVTVKVVKGMITEVRTKGEPAPANDPKS
                     "
     gene            562..>1022
                     /locus_tag="PLH31_20675"
     CDS             562..>1022
                     /locus_tag="PLH31_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQR91641.1"
                     /translation="MLTLSRWKIIVVTLAVIFGLVFTLPNLLPQRALDALPGWVPKQK
                     LNLGLDLQGGSYLLMEVDTAALRRERLTNLVEDVRTSLRGEQIAFGELREAGGVISVR
                     IADAAKVNDAANLLRQSVGSPLVGAVGGRDISVATRDNQRLEISFVAEAAKA"
BASE COUNT          183 a          332 c          328 g          179 t
ORIGIN      
        1 ggcgtcgtcg gcgcggccgg cgccaggcgc tccagggcgt cggcgatgcg ggcgagcagg
       61 gcgttttggg cgtcgatcat ggcccggtcc taaccttgcc gcgccgtcat tgcaaaagag
      121 gaggtgaacg catagcttcc gccgacttgg cgagacccca tatgggggtc ccgaccgcag
      181 aaattttgga gacgtcatgc ccggtggcct gagcgcgcag cttatgcaaa tcgcccccat
      241 ccttctgatg gtggttctgt tctacttcat gctgatccgt ccgcagcaga agcgggccaa
      301 agagcatcag atcatgctgt ccaacctgaa gcgcggcgac aatgtcgtgc tgtccagcgg
      361 catgctcggc aagatcgttc gtgtggaaga caaggaagtc ggcctcgaga tcgctcaggg
      421 cgtcaccgtg aaggtcgtca agggcatgat caccgaggtc cgcaccaagg gcgagcccgc
      481 cccggccaac gaccccaaga gctgatttcc caaactcgcc gaccacagac gccgcaaggc
      541 gtcgtccaga gcctgtaatc catgctgaca ctgtcccgct ggaagatcat cgtcgtcacc
      601 ctggcggtga tcttcggcct cgtcttcacc ctgccaaacc tgctgccaca acgggcgctt
      661 gatgctctgc ccggctgggt tcccaagcag aagctcaatc tgggcctcga cctgcagggc
      721 ggatcctacc tgctcatgga ggtggatacg gccgcgctgc gtcgcgaacg tctgacgaat
      781 ctcgtcgagg acgtccgcac cagcctgcgg ggcgagcaga ttgcgtttgg cgagctgcgt
      841 gaagcgggcg gcgtgatcag tgtgcgcatc gccgacgccg ccaaggtcaa tgacgccgcc
      901 aacctgctgc ggcagtcggt gggctcgccc ctggtcggcg ccgtcggcgg tcgcgacatc
      961 tcggtcgcca cgcgcgacaa ccagcgtctg gagatcagct tcgtcgccga ggccgccaag
     1021 gc
//
LOCUS       DAOMEO010001307         1021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14854_length_1021_cov_2.928571, whole genome shotgun sequence.
ACCESSION   DAOMEO010001307 DAOMEO010000000
VERSION     DAOMEO010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14854_length_1021_cov_2.928571"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLH31_20680"
     CDS             complement(<1..247)
                     /locus_tag="PLH31_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQR91642.1"
                     /translation="MTGTIEADYIVVGAGSAGCVLAARLSENGRFKVLLLEAGGDDRP
                     TKNLSQFASNLMIHIPVGYAQTLKDPKVNWLYTTEPDP"
     gene            483..>1021
                     /locus_tag="PLH31_20685"
     CDS             483..>1021
                     /locus_tag="PLH31_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR91643.1"
                     /translation="MDLISQTVVDGKAGPGAGTTYPTLPDIDLADIFRFAKGQPFADY
                     ARMRVEAPVMWHPEPYGGPGFWAVTRHDDVMKVNGDPTTYSSQRGGILMAMGPPDRRH
                     ALLFRASMDTMINLDAPHHLQLRREHMPYFTPSYLRGITEAVKGEVTRLLDAMEPLLE
                     GGATIDMVEHFSSVLPLFTL"
BASE COUNT          161 a          339 c          330 g          191 t
ORIGIN      
        1 ccgggtcggg ctcggtggtg tagagccagt tgaccttggg gtccttcagg gtctgggcgt
       61 agccgaccgg gatgtggatc atcaggttgg aggcgaactg cgagaggttt ttcgtgggcc
      121 tgtcgtcgcc gccggcttcc agcagcagca ccttgaagcg cccgttctcc gacagtcgcg
      181 cggccagcac gcagccggcc gaaccggcgc cgacaacgat gtagtccgcc tcgatggtcc
      241 cggtcatcgt cttctcccaa gggcgcgctt gggcgcgctc catttttgtg tttcgagccc
      301 ttcccattcg gaaggcccgg ggccgcccgc gtgtgcccgc gtgatcgacg ttcactcaca
      361 atgcgcggga cttggcggaa cgcaaagcct caccttgcgt cagttgccgt cgcggcgccg
      421 ttggcgcgcc atttttaaag gctatcgtcc tcgtcaaaga acgatgtttc cgggagagat
      481 gcgtggatct gatcagccag acagtggtgg acggcaaggc cggccccgga gcgggaacca
      541 cctatccgac cctgccagac atcgatctcg ccgacatctt ccgcttcgcc aagggccagc
      601 cctttgccga ctatgcgcgg atgcgggtcg aggcgccggt gatgtggcac cctgagccct
      661 atggcgggcc gggcttctgg gcagtgacgc gccacgatga cgtgatgaag gtcaacggcg
      721 acccgacgac ctattcctcg cagcgcggcg gcatcctgat ggccatgggc ccgcccgacc
      781 gccgtcacgc cctgctgttc cgggcgtcga tggacaccat gatcaatctc gacgcaccgc
      841 atcacctgca gctgcgtcgc gagcacatgc cctatttcac gccgtcctat ctgcgcggca
      901 tcaccgaggc cgtgaagggc gaggtgaccc gcctgctgga cgccatggag ccgctgctgg
      961 aaggcggggc gaccatcgac atggtcgagc acttctccag cgtgctgccg ctgttcaccc
     1021 t
//
LOCUS       DAOMEO010001308         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14868_length_1020_cov_2.449741, whole genome shotgun sequence.
ACCESSION   DAOMEO010001308 DAOMEO010000000
VERSION     DAOMEO010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14868_length_1020_cov_2.449741"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLH31_20690"
     CDS             <1..810
                     /locus_tag="PLH31_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HQR91644.1"
                     /translation="LAGVDTRALTRKIRETGMPHGVIAHSPDGVFDLDALVAKAKAWS
                     GLEGLDLAKDASTTQTFTWDEGLWSWPEGYAKLDKPKYEVVVLDYGVKRNILRALAHV
                     GARATVVPANTSAEDILARNPDGVLLSNGPGDPAATGEYAVPEIQKLVASGKPVFGIC
                     LGHQMLALALGAKTVKMEQGHHGANHPVKDLTTGKVEIVSMNHGFTVDSASLPAPVTE
                     THVSLFDGTNAGIQLTDKPVFSVQHHPEASPGPTDSLYLFERFAGLMDAAK"
BASE COUNT          183 a          354 c          334 g          149 t
ORIGIN      
        1 ctggccggcg tcgacacccg cgccctgacc cgcaagatcc gcgagaccgg catgccgcat
       61 ggcgtgatcg cccacagccc ggacggcgtg ttcgacctgg acgccctggt cgccaaggcc
      121 aaggcctggt caggtctgga aggcctggac ctggccaagg acgcctcgac cacccagacc
      181 ttcacctggg acgagggcct gtggtcgtgg ccggaaggct acgccaagct ggacaagccc
      241 aaatacgagg tcgtggtgct cgactacggc gtcaagcgca acatcctgcg ggccctggcc
      301 catgtcggcg cccgcgccac ggtggtgccg gccaacacct cggccgaaga cattctggcc
      361 cgcaatcccg acggcgtgct gctgtccaac ggcccgggcg acccggccgc gaccggcgag
      421 tacgcggtgc ccgagatcca gaagctggtc gccagcggca agccggtgtt cggcatctgc
      481 ctgggccacc agatgctggc cttggcgctt ggcgccaaga cggtgaagat ggaacagggc
      541 caccacgggg ccaatcaccc ggtgaaagac ctgaccaccg gcaaggtcga gatcgtctcg
      601 atgaaccacg gcttcacggt cgatagcgcc agcctgccgg ccccggtcac cgagacccat
      661 gtctcgttgt tcgacggcac caacgccggc atccagctga ccgacaagcc ggtgttcagc
      721 gtccagcatc acccggaagc ctcgccgggt ccgacggaca gcctctatct gttcgagcgc
      781 ttcgccggcc tcatggacgc ggcgaagtag accttcgctg agagaacggc gggatttgaa
      841 aatcccgccc gatccggcaa tctctcccaa actcggggga gggcgccatg acggccgaag
      901 tgaaaaccga tgaactcggc gcgccggcca cggggccgcg cgggcggctc gaggcggtcg
      961 acatgttgcg gggtctggtc atggtcgtca tggccctgga ccatctgcgc gacttctttc
//
LOCUS       DAOMEO010001309         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14870_length_1020_cov_2.037306, whole genome shotgun sequence.
ACCESSION   DAOMEO010001309 DAOMEO010000000
VERSION     DAOMEO010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14870_length_1020_cov_2.037306"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..301)
                     /locus_tag="PLH31_20695"
     CDS             complement(29..301)
                     /locus_tag="PLH31_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91645.1"
                     /translation="MTNSMWKAALCAGVSLVCLPSIASAQSGSQPDPKPTTGLEEIIV
                     TAQRREERLQDVPVSVTAFGAKELERRQITDVRALTENAPSITFTA"
     gene            complement(488..>1020)
                     /locus_tag="PLH31_20700"
     CDS             complement(488..>1020)
                     /locus_tag="PLH31_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR91646.1"
                     /translation="VPIGTSGEVVCRPLMPDIIFKGYWRRPEDTLKVMGNLWFHTGDI
                     GKFDENGFFYFVDRKKDYLRRRGENISSFEMETSILTHPEIEQVAVHAVPSNLQEDDL
                     KVTAKLKPGAVLSEEELCRWLIERVPYYAVPRYIEFRAELPVNPQGRVLKFQLRDEGA
                     TPTTWDIETTDIKPSR"
BASE COUNT          186 a          317 c          315 g          202 t
ORIGIN      
        1 agatcggcgc catccaatcc accaccgatc atgcggtgaa ggtgatcgac ggcgcattct
       61 cggtcagggc gcgaacgtcg gtgatctgac gacgctccag ctccttcgcg ccaaaggccg
      121 tcaccgagac cggcacgtcc tgcagccgtt cttcccggcg ctgggccgtg acgatgatct
      181 cttcgagacc ggtggtcggt ttcgggtcgg gctgagaccc gctctgggcg ctagcgatcg
      241 agggaagaca gaccagcgat acccccgcgc aaagcgcggc tttccacatg gagtttgtca
      301 tgatcggccc tccccgagcc gctgcgctcg gcggccactg gaacgagccc taagggactc
      361 ccgcggccgc ctgttggcga gagaggagcg cgatcacgag tctaagtcaa tcgatttagt
      421 ctgatgtcaa aatatcattc tatattcgat atgactcatg acgccttgcg gcgacggaaa
      481 gccagcgtca tcggctgggt ttgatgtcgg tggtttcgat atcccaggtg gtcggggtcg
      541 ctccttcgtc gcgaagctgg aacttgagca cccgcccctg cggattgacc ggcagctcgg
      601 cccggaactc gatgtagcgt ggcaccgcgt agtagggcac gcgctcgata agccagcggc
      661 acagctcctc ctcggacagg acggccccgg gcttgagctt ggcggtcacc ttcaggtcgt
      721 cttcctggag gttcgacggc acggcatgaa cggcgacctg ttcgatctcg ggatgagtca
      781 ggatcgaggt ctccatctcg aagctcgaga tgttctctcc gcgccgtcgc aaatagtcct
      841 tcttgcggtc gacgaaatag aagaagccgt tctcatcgaa cttgccgatg tcgccggtgt
      901 ggaaccacag gttccccatg accttcagag tgtcctcggg ccgccgccag taccccttga
      961 agatgatgtc gggcatcagc gggcggcaca ccacctcgcc ggacgttccg atcgggacct
//
LOCUS       DAOMEO010001310         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14893_length_1019_cov_2.354772, whole genome shotgun sequence.
ACCESSION   DAOMEO010001310 DAOMEO010000000
VERSION     DAOMEO010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14893_length_1019_cov_2.354772"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLH31_20705"
     CDS             <1..871
                     /locus_tag="PLH31_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91647.1"
                     /translation="DREAQQGRPLDKKALEALFREFGHPLAVAGRYGPQRALIGAELY
                     PFYIFAVKVALAIAAFVAVIPAVFGAVTSHGDVSRAIGHAIHAFIPSALTLIGAITII
                     GAMIERGWIKTGEFGQWRVSDLPHISTKKQWFRTTRFEALFEVIFMTFFALWWAGVVD
                     FPGSRGPFGHDGELQITLAPILTQLHTPILLLAIGQALSGLILVTRPAWIAPRAVAEI
                     VLSIAGIALAAVLWGQMPLVTFAGPGASAEDFVTLRHVADNIFRGCVLVTVAICAGKI
                     LIEGWRLLRLTFR"
BASE COUNT          133 a          363 c          342 g          181 t
ORIGIN      
        1 ggaccgggag gcccagcagg ggcgtcccct cgacaagaag gcgctggagg ccctgttccg
       61 agagtttggc catccgctgg ccgtggctgg acgctatggc ccccagcgcg ccctgatcgg
      121 ggcggagctc tatcccttct acatcttcgc ggtgaaggtc gccctggcca tcgcggcgtt
      181 cgtggcggtg atcccggcag tgtttggcgc cgtcaccagc catggcgatg tctcgcgcgc
      241 catcggtcac gcgatccacg ccttcattcc cagcgccctg acgctgatcg gagccatcac
      301 catcatcggg gcgatgatcg agcgcggctg gatcaagacc ggcgagttcg gccagtggag
      361 ggtcagcgac ctgccgcaca tctcgacgaa gaagcagtgg ttccgcacca cgcgcttcga
      421 ggccctgttc gaggtcatct tcatgacctt cttcgccctg tggtgggccg gcgtcgtgga
      481 ctttcccggc agccgggggc cctttggcca tgacggcgaa ctgcagatca cgctggcgcc
      541 gatcctgacc cagttgcaca cgccgatcct gctgctggcc attggtcagg cgctgtccgg
      601 cctgatcctg gtgacccgcc cggcctggat cgcgccgcgc gccgtcgccg agatcgtgct
      661 gtcgatcgcc ggaatcgccc tggccgccgt cctgtggggc cagatgccgc tggtgacatt
      721 cgccgggccg ggcgcctcgg ccgaggactt cgttaccctt cgccatgtcg ccgacaacat
      781 cttcaggggc tgcgtgctgg tcacggtcgc catctgcgcc ggcaagatcc tgatcgaggg
      841 ctggcggctg ctccggctca cgttccgata gacgggcccg gctgagctgt caggcggcga
      901 ccggggctat ttccttggct tggaggcggt gtcgaccacg gccccgacgc cggccccgac
      961 caccgcgccg accggtccgc cgaccacggc gccagccgcc gcgccggtgg ctgcccgct
//
LOCUS       DAOMEO010001311         1019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14895_length_1019_cov_2.231328, whole genome shotgun sequence.
ACCESSION   DAOMEO010001311 DAOMEO010000000
VERSION     DAOMEO010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14895_length_1019_cov_2.231328"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLH31_20710"
     CDS             <1..233
                     /locus_tag="PLH31_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR91648.1"
                     /translation="AIAAGSTMPPAGAGSRQTIGSVIFTGAIFATLLTLFVVPVFYNL
                     LARFTKSPEWTSRQIEDYEAREKSGEGQSSPA"
     gene            248..>1019
                     /locus_tag="PLH31_20715"
     CDS             248..>1019
                     /locus_tag="PLH31_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HQR91649.1"
                     /translation="MPDGSMTAKRQEIALLRRIEERILWLASWTIHNANHLRESRDGL
                     KVGGHQASCASMTTLMTALYMKALRPQDRVAVKPHASPVFHAIQHLFGRQSLDNLKRF
                     RSLGGAQSYPSRTKDIDDVDFSTGSVGLGVAMTAFASLAQDYLAARGAVKPEAMGRMI
                     SLLGDAELDEGNIYEALIEACKHDVRNTWWIVDYNRQSLDATTQDRMFTRYGEIFEAA
                     GWTVETLKWSKRQREVFAKPGGEALRIWIETAPNDLYSA"
BASE COUNT          188 a          340 c          331 g          160 t
ORIGIN      
        1 gcgcgatcgc ggccggaagc acgatgccgc ccgcaggggc gggcagccgc cagacgatcg
       61 gctcggtgat cttcacgggc gcgatcttcg ccaccctgct gaccctgttc gtggtgccgg
      121 tgttctacaa cctgctggcg cgcttcacga agtctccgga gtggacgtcg cggcagatcg
      181 aggactatga agcccgagag aaaagcggtg aggggcaatc ctcaccggca taaaccggtg
      241 aggaagcatg ccagacggct cgatgaccgc gaagcggcag gagattgcgc tccttcgccg
      301 tatcgaggaa cgcattctct ggctggcctc gtggacgatc cacaacgcca atcacctgcg
      361 tgaaagccgg gacggcctga aggtgggcgg ccatcaggcc tcctgcgcct caatgaccac
      421 cctgatgacc gcgctctaca tgaaggcgct gcgaccccag gaccgcgtgg cggtcaagcc
      481 tcacgccagc ccggtgttcc acgccatcca gcacctgttc ggccgccaga gcctggacaa
      541 cctcaagcgc ttccggtcgc tgggcggggc gcagtcctat ccctcgcgca ccaaggacat
      601 tgacgacgtc gacttctcga ccggctctgt gggcctgggc gtggccatga ccgccttcgc
      661 ctcgctggcg caggactatc tggctgcgcg gggtgcggtg aagcccgaag ccatggggcg
      721 gatgatctcg ctgctgggcg atgccgagct ggacgagggc aacatctacg aagccctgat
      781 cgaggcctgt aagcacgacg tccgcaacac ctggtggatc gtcgactaca atcgccagag
      841 cctggacgcg acgacccagg accggatgtt cacccgctat ggcgagatct tcgaggccgc
      901 cggctggacc gtcgagacgc tgaaatggtc caaacgccag cgcgaggtct tcgccaagcc
      961 cggcggcgag gcgctgcgca tctggatcga gaccgcgccc aacgacctct attcggccc
//
LOCUS       DAOMEO010001312         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14921_length_1018_cov_2.410177, whole genome shotgun sequence.
ACCESSION   DAOMEO010001312 DAOMEO010000000
VERSION     DAOMEO010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14921_length_1018_cov_2.410177"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLH31_20720"
     CDS             complement(<1..761)
                     /locus_tag="PLH31_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQR91650.1"
                     /translation="MRTHDADVIIAGAGMAGSTLALALASGGLKPLLIDPQPFEAQLA
                     PTFDGRSSAIAYSSFRQWRTVGAGETLAPHAQRIEQILVTDGRTPSASARGASPAFLR
                     FDSSEIADRSDGEPLGYLIENRQIRAALSQAVTEKAIPVIAPMAATNLEVDAEGARLT
                     LADGRVLSAPLVVSAEGRGAALRKAAGIGEIGWGYGQSGVVVTVKMEHPHEGVAHEYF
                     LPSGPFAILPLTENRASLVWTESTARAEALRGARP"
     gene            890..>1018
                     /locus_tag="PLH31_20725"
     CDS             890..>1018
                     /locus_tag="PLH31_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQR91651.1"
                     /translation="MSVTLDLSRVSGAVPATKPLINLSGLTRPQMIAALTESGVAEH"
BASE COUNT          152 a          356 c          357 g          153 t
ORIGIN      
        1 tcgggccgcg cgccgcgaag agcctcggcc cgggcggtgc tctcggtcca gaccaggcta
       61 gcccgatttt cggtcagagg caggattgcg aacgggccgc tgggcaggaa gtactcatgg
      121 gccaccccct cgtgcggatg ctccatcttc accgtgacca cgacgccgct ctggccgtag
      181 ccccagccga tctcgccgat gcccgccgcc ttgcgcaggg ccgcgccccg gccctcggcg
      241 ctgaccacca gcggggcgct cagcacccgg ccgtcggcca gggtcagacg agccccttca
      301 gcgtcgactt cgaggttggt cgcggccatc ggcgcgatca ccgggatggc cttctcggtc
      361 acggcctggg acagggccgc gcggatctgg cgattctcga tcagatagcc caggggctcg
      421 ccgtcggagc ggtcggcgat ctcgctggaa tcaaagcgca ggaaggccgg cgaggcgccc
      481 cgcgcgctgg cgctcggcgt gcggccgtcg gtgaccagga tttgctcgat ccgctgggcg
      541 tgcggggcca aggtctcgcc cgcgccaacg gtgcgccact ggcgaaagct ggaataggcg
      601 atggccgacg agcggccgtc gaaggtcggg gccaactggg cctcgaatgg ttgtgggtcg
      661 atcaacaggg gtttcaaacc gcctgaggcc agggccaggg ccagggtgga tccggccatg
      721 ccggctcccg cgatgatcac atcggcgtcg tgcgtgcgca tgaggcggat atgggcctga
      781 ggggcggggg tgtctagagc cctgcgacgg acggcctttt ggaaaaaccc ccgccaagtg
      841 ctatatgccg ccctcgaccc cataaatcct cccgaccccg gatcgcccct tgagcgtcac
      901 cctcgatctg tcgcgcgtct caggcgccgt ccccgcgacg aagccgctga tcaacctgtc
      961 gggcctgacc cggccgcaga tgatcgccgc cctgaccgag tcgggcgtgg ccgagcac
//
LOCUS       DAOMEO010001313         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14922_length_1018_cov_2.331256, whole genome shotgun sequence.
ACCESSION   DAOMEO010001313 DAOMEO010000000
VERSION     DAOMEO010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_1018_cov_2.331256"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1018
                     /locus_tag="PLH31_20730"
     CDS             <1..>1018
                     /locus_tag="PLH31_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002519095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HQR91652.1"
                     /translation="AAPSDMDAEALERALFLCRKRIEKQVQAQNLSGFYICSLSAKSL
                     IYKGMFLAEAIDEFYPDLKDERFAANVAIFHQRYSTNTFPEWRLAQPFRMLAHNGEIN
                     TIKGNINWMKSHEIKMAADAFGAFGDDVKPVIQPGGSDSASLDNTFEVLVRAGRDAPM
                     AKTLLMPEAASPNLKPSHTALYSYCNAVMEPWDGPAALCATDGRWVVAGKDRSGLRPL
                     RVAYTDDGLVIMGSEAGMCGVAESRITRKLAIAPGRMIAIDLAEGRLYGEDEIIDELA
                     GRHPYTEWLGNMVDLEKEIAPGPEPRSYGREELTRRQAAAGFSLEDLEMILAPMVEDG
                     KEAVG"
BASE COUNT          187 a          349 c          320 g          162 t
ORIGIN      
        1 ggccgcgccc agcgatatgg acgccgaagc tctggagcgc gccctgttcc tgtgtcgcaa
       61 gcggatcgaa aagcaggtcc aggcccagaa tctgtcgggt ttctacatct gctccctgtc
      121 agccaagtcg ctgatctaca agggcatgtt cctggccgag gcgatcgacg agttctaccc
      181 cgacctgaag gacgagcgct tcgccgccaa tgtggcgatc ttccaccagc gctattcgac
      241 caacaccttc cccgaatggc gcctggccca gcctttccgg atgctggccc acaatggtga
      301 gatcaacacc atcaagggca acatcaactg gatgaagtct cacgagataa agatggcggc
      361 cgacgcgttc ggcgctttcg gggacgatgt taagccggtg atccagccgg gcggttcgga
      421 ttccgccagc ctggacaata ccttcgaggt gctggtccgg gccggccgcg acgcgcccat
      481 ggccaagacc ctgctgatgc ccgaggccgc cagccccaac ctcaagccca gccacaccgc
      541 gctctattcc tactgcaatg ccgtgatgga gccgtgggac ggcccggccg ccctgtgcgc
      601 caccgacggc cgctgggtcg tggccggcaa ggaccgctcg ggcctgcggc cgctgcgcgt
      661 ggcctatacc gacgatggcc tggtgatcat gggctcggaa gcgggcatgt gcggcgtcgc
      721 cgagagccgc atcacccgca agctggccat cgcgccgggc cggatgatcg ccatcgatct
      781 tgccgaaggc cgcctctatg gcgaggacga gatcatcgac gaactggccg gccgccaccc
      841 ctataccgag tggctgggca acatggtcga tctcgaaaag gagatcgccc ccggtcccga
      901 gccccgcagc tatggccgcg aggaactgac ccgccgccag gccgccgccg gcttcagcct
      961 tgaagacctg gaaatgatcc tggcccccat ggtcgaggac ggcaaggagg ccgtcggc
//
LOCUS       DAOMEO010001314         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14948_length_1017_cov_2.252599, whole genome shotgun sequence.
ACCESSION   DAOMEO010001314 DAOMEO010000000
VERSION     DAOMEO010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14948_length_1017_cov_2.252599"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PLH31_20735"
     CDS             complement(<1..>1017)
                     /locus_tag="PLH31_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR91653.1"
                     /translation="MVPALEQIIKRGGSLGVKDIVLGMPHRGRLNVLAAVMGKPYHVI
                     FHEFQGGTSLPSDVEGSGDVKYHMGASSDREFDDNKVHLSLTANPSHLEIVNPVVIGK
                     ARAKQAFTLREQPDAGRGHVLPLLLHGDAAFAGQGVVAECFTLSGLKGYRTGGTIHFI
                     VNNQIGFTTSPRYSRSSPYPSDVALMVEAPIFHVNGDDPEAVVYAAKVATEYRQMFGK
                     DVVVDMFCYRRFGHNEGDDPTMTSPLMYAKIKGHPSTQELYSKRLIGEGVITQGDVDG
                     WINEFEAFLDKEFDAGKTYKANKADWLDGKWAGLATPADEERRGSTAFPRARLLELGR
                     QITTI"
BASE COUNT          162 a          320 c          352 g          183 t
ORIGIN      
        1 gatcgtcgtg atctggcgac ccagctccag caggcgggcg cgcgggaagg ccgtcgagcc
       61 gcgacgctcc tcatcggcgg gggtggccag gcctgcccac ttgccgtcca gccagtcggc
      121 cttgttggcc ttgtaggtct tgccggcgtc gaattccttg tcgaggaagg cctcgaactc
      181 gttgatccag ccgtcgacat cgccctgggt gatcacgccc tcgccgatca ggcgcttgga
      241 atacagctcc tgggtcgagg ggtggccctt gatcttggcg tacatcagcg gcgaggtcat
      301 cgtcgggtcg tcaccttcgt tgtgaccgaa ccggcggtag cagaacatgt cgaccacgac
      361 gtccttgccg aacatctggc gatattcggt ggcgaccttg gccgcgtaga ccacggcttc
      421 gggatcgtcg ccgttgacgt ggaagatcgg ggcctcgacc atcagcgcca catccgaggg
      481 atagggcgag ctgcgcgaat agcgcgggct ggtggtgaaa ccgatctggt tgttgacgat
      541 gaagtggatg gtgccgcccg tgcggtagcc cttcaggccc gacagggtga agcactcggc
      601 caccacgccc tggccggcaa aggcggcgtc gccatgaagc agcagcggca gcacgtggcc
      661 gcggccggcg tccggctgtt cgcgcagggt gaaggcctgc ttggcgcggg ccttgccgat
      721 cacgacaggg ttgacgattt ccaggtgcga cgggttggcc gtcagcgaca ggtggacctt
      781 gttgtcgtcg aactcacggt ccgacgaggc gcccatgtga tacttgacgt cgcccgaccc
      841 ctcgacgtcc gagggcaggc tggtgccgcc ctggaactcg tggaagatca cgtgataggg
      901 cttgcccatc acggcggcca gcacgttcag gcggccgcgg tgcggcatgc ccaggacgat
      961 gtccttcacg cccagcgaac cgccgcgctt gatgatctgc tccagcgccg ggaccat
//
LOCUS       DAOMEO010001315         1017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14961_length_1017_cov_1.539501, whole genome shotgun sequence.
ACCESSION   DAOMEO010001315 DAOMEO010000000
VERSION     DAOMEO010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_1017_cov_1.539501"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PLH31_20740"
     CDS             complement(<1..>1017)
                     /locus_tag="PLH31_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91654.1"
                     /translation="ILDKVMASIRRERTKEVAAEKAKIRAEVTDEMMAEPGQAALHLL
                     RKGKLYSGETPEVLVGAKLDRAALVEMLGEAGIANLPPGIFREEGIDPDVLAQAVGLG
                     NGVELVDRLMQIQAEQQAMRAKGDNRSMLAARIAEETQKRLTDSLGDPLNDGSIEAEA
                     MAAVHSDKQGALMSTELKVLARKAKQSGAISLSDIEDWAAQTISEMSVYQATQHAKYQ
                     RAERMAGQKVQRALIKGDFVAAFKAKQDQMVNFALYREARKAADDVESIRKLADRYGS
                     AATVKSMDQASLEQIHQVLEDYDFKKRSGTLLAERTTFAMWAAEQAAAGFEVIEPPRL
                     KGAG"
BASE COUNT          140 a          358 c          298 g          221 t
ORIGIN      
        1 aacccagcgc ccttgaggcg cggaggctct atcacctcga aacccgccgc cgcttgttcc
       61 gccgcccaca tggcgaaggt ggtgcgttcc gccagcaggg tcccgctgcg cttcttgaag
      121 tcgtagtctt ccagtacctg atggatctgc tccagagagg cctgatccat gctcttgacg
      181 gtcgcggccg agccgtagcg atccgccagc ttgcggatgc tttcgacgtc gtcggccgcc
      241 ttccgggcct cgcggtacag cgcgaagttg accatctggt cttgcttcgc cttgaaggct
      301 gccacgaagt cgcccttgat cagcgcccgc tggaccttct gcccggccat gcgttcggcg
      361 cgctgatact tcgcgtgctg cgtcgcctga tagaccgaca tttcggagat cgtctgggct
      421 gcccaatcct cgatatcgga gagcgagatc gcgcccgact gcttggcttt gcgcgccagc
      481 accttcagtt cggtggacat cagcgcgccc tgcttgtcgg aatgcacggc cgccatggct
      541 tctgcttcga tgctcccgtc gttcagcggg tcgcccaggc tgtccgtcag ccgcttctgt
      601 gtctcctctg cgatccgggc cgccagcatg gagcggttgt cgcccttggc gcgcatggcc
      661 tgctgttcgg cctgaatttg catcagccgg tcgactagct cgacgccgtt tccgaggccc
      721 acggcctgag ccagtacgtc cggatcgatc ccctcttcac ggaagatgcc cggcggcaga
      781 ttggcgatcc ccgcttcgcc cagcatttcc accagcgcgg cacgatccag cttcgcgccg
      841 accagaacct ccggcgtctc cccggaatag agcttgccct tgcgcagcag gtgcagcgcg
      901 gcctgtcccg gttcggccat catttcgtcg gtgacttcgg ccctgatctt ggccttctct
      961 gccgcgacct ctttcgtccg ctcgcgccga atcgaagcca tgaccttgtc gagaatg
//
LOCUS       DAOMEO010001316         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_14971_length_1016_cov_2.261186, whole genome shotgun sequence.
ACCESSION   DAOMEO010001316 DAOMEO010000000
VERSION     DAOMEO010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14971_length_1016_cov_2.261186"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1016)
                     /locus_tag="PLH31_20745"
     CDS             complement(5..>1016)
                     /locus_tag="PLH31_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HQR91655.1"
                     /translation="GLALRPMLEAFTPADLVAWAQGLGQETFVGASGRVFPKVMKASP
                     LLRAWLARLEGQGVTLRTQAEWLGWDPDGALMFRTPNGLEAVRCRVTILALGGASWAR
                     LGSDGAWTPLLAAKGSGLAPFQAANCGFDVAWSAVFRDRFAGEPLKNVALRLGQAEGR
                     GDAMIAGYGLEGGAVYALGAAPREAIAATGRAVVEIDLRPDIPLETLAARLSRPRGGQ
                     SLANFLRKAAHLSPVEVNLLRQAHGLDLPAEPSALAAAIKAAPITLSGVQPLDRAISS
                     AGGVTLDSLSGLELKAAPGVFVAGEMLDWEAPTGGYLLQACFATGVAAARQVLARLER
                     HE"
BASE COUNT          159 a          385 c          348 g          124 t
ORIGIN      
        1 aagatcattc atgccgttcc agccgcgcca ggacctgccg agccgccgcg acgccagtgg
       61 cgaaacaggc ctgcagcaga tagcccccgg tgggcgcttc ccagtccagc atctcgccag
      121 cgacgaacac gccgggcgcc gccttcaatt ccagcccgct gagcgagtcc agcgtcacgc
      181 cgccagccga cgagatcgcc cggtccagcg gctggacgcc gctcagggtg atcggcgcgg
      241 ccttgatcgc cgccgccagg gccgagggct cggccggcag gtccaggcca tgggcctggc
      301 gcagcagatt gacctcgacc ggcgacaggt gcgcggcctt gcgcaggaaa ttggccaggg
      361 actggcctcc gcgcggtcgc gacagtcggg cggccagggt ctcgagcgga atgtctggcc
      421 gcaggtcgat ctcgaccacc gcccggccgg ttgcggcgat agcctcgcgc ggcgcggcgc
      481 ccagagcgta gaccgccccg ccctctaggc cgtagccggc gatcatggcg tcgccccgtc
      541 cctcggcctg acccagccgc agggcgacgt tcttcaaagg ctcgccggcg aagcgatccc
      601 gaaagacagc gctccaggcg acgtcgaatc cgcaattggc ggcctggaac ggcgccagac
      661 ccgagccctt ggcggccagt aggggcgtcc aggcgccgtc cgagccgagc ctggcccagc
      721 tggcgccgcc cagggccagg atcgtgacgc ggcatcgtac ggcctcaagg ccgtttggcg
      781 tgcggaacat caaggcgcca tcggggtccc agcccagcca ttcggcctgt gtgcgcagcg
      841 tgacgccctg cccctccagc cgcgccagcc acgcccgcag caggggcgag gctttcatca
      901 ccttgggaaa cacccggccg ctggccccga cgaaggtctc ctgccccagg ccctgcgccc
      961 aggcgaccag atcggcgggc gtgaaggcct ccagcatggg gcgcagcgcc agaccc
//
LOCUS       DAOMEO010001317         1013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15030_length_1013_cov_3.147182, whole genome shotgun sequence.
ACCESSION   DAOMEO010001317 DAOMEO010000000
VERSION     DAOMEO010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_1013_cov_3.147182"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..>1013)
                     /locus_tag="PLH31_20750"
     CDS             complement(262..>1013)
                     /locus_tag="PLH31_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR91656.1"
                     /translation="DPKAGRLSTASADRLLAAVAEQNLNLHWILETHAHADHLSAADH
                     LRAVTGARIVIGEQITQVQKGFGDLFEADDVDPDGGVFDLLVRDGDQIPLGGRMIEVL
                     HTPGHTPACVSYRIDDAIFVGDTLFMPDYGTARADFPGGDAATLYRSIRKILALPPRT
                     RIFIGHDYLPAGRETFAWETTVAEQRAANVHVHDGVEETDFVAMRQARDATLAAPTLI
                     LPALQVNIRAGALPPASPSGRVFLKLPVNAL"
BASE COUNT          171 a          344 c          341 g          157 t
ORIGIN      
        1 cttgggtatc agagctgaaa gacgctcgct tggcccgatc ctcacgcaag aacagtttct
       61 gcgaagcgga caggtcgagc cgaaacgcaa atcatcatca cggtcatccg ggttcgcccc
      121 ggccgcttcg cgcctcccca cctaactcag cggtttgccg cgtgaggctg agaccgcatg
      181 tccgatgccc cctggggagc tctcccaaag cgacagaggg gcgccccgac gcgaccatga
      241 tccggagccc ctcccctgcc cctacagcgc gttcaccggc agcttcagaa acacccgacc
      301 actgggcgag gccggcggga gggcgccggc gcggatgttc acctgcaggg ccggcaggat
      361 cagggtgggg gccgccaggg tggcgtcgcg ggcctggcgc atcgccacga agtcggtctc
      421 ttccacgccg tcatgcacat ggacattggc cgcccgctgc tcggcgacgg tggtttccca
      481 cgcgaaggtc tcgcgcccgg ccggcagata gtcgtgaccg atgaagatcc gcgtccgggg
      541 cggcagggcc agaatcttgc ggatcgagcg atagagcgtg gccgcatcgc cgccggggaa
      601 gtccgcccgc gccgtgccgt agtccggcat gaacagggtg tcgcccacga agatggcgtc
      661 gtcgatccgg tagctgacac aggccggggt atggcccggc gtgtgcaaca cctcgatcat
      721 gcgtccgccc agcgggatct ggtcgccgtc cctgaccaac aggtcgaaga caccgccgtc
      781 cggatcgacg tcgtcggctt cgaacaggtc gccaaagccc ttctggacct gggtgatctg
      841 ttcaccgatc acgatgcggg cccccgtcac ggcgcgcagg tggtcagccg ccgaaaggtg
      901 gtcggcatgg gcgtgggttt ccagaatcca gtgcaggttg aggttctgct cggccaccgc
      961 cgccagcagc cgatcggccg aagcggtcga cagccgaccc gccttggggt cga
//
LOCUS       DAOMEO010001318         1013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15035_length_1013_cov_2.049061, whole genome shotgun sequence.
ACCESSION   DAOMEO010001318 DAOMEO010000000
VERSION     DAOMEO010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15035_length_1013_cov_2.049061"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>1013
                     /locus_tag="PLH31_20755"
     CDS             47..>1013
                     /locus_tag="PLH31_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQR91657.1"
                     /translation="MREKLFIGPKVRRLRLALGWKLEPCATRLGLSVSYLSQIEANQR
                     PVTARVLLDMMRVFEVDATALDAADDTRLIADLREAIADQQDQEQAPGLGELKQAVAN
                     TPNLARSYLALHHAYRRLDERLKATEEAVSLSETVAASALLPYEEVRDFFHYKNNYIH
                     SLDVAAEALSEKLGLASDVSAETALERHLGASLGITVARSMEPGLLRRFDRVSGTLTL
                     GAADAATTRTFQMAYQIVSEGLSDAVESELRAAGFRSESAAKVCRTALFNYAAGALLL
                     PYERFRAAARAARHDIEHLSLTFQTSLEQVCHRLSTLQRPGARGVP"
BASE COUNT          178 a          319 c          334 g          182 t
ORIGIN      
        1 ggtgtgtctt gtgtggtaaa attctgtaaa acggtaaaat tgtaaaatgc gcgagaagct
       61 gttcatcggt cccaaggtgc gtcgactgcg tctggctctc ggctggaagc tggaaccctg
      121 cgccacacgc ctgggtctgt ccgtcagcta tctgtcgcag atcgaggcca atcagcgacc
      181 ggtgacggcg cgggtgctgc tcgacatgat gcgcgtgttt gaggtcgacg ccacggcatt
      241 ggacgccgcc gatgacacgc gcctgatcgc cgatctgcgg gaggccatcg ccgaccagca
      301 ggaccaggag caggctccgg gcctggggga gctcaagcag gccgtggcca atacgcccaa
      361 cctcgcccga tcctatctgg ccctgcacca cgcctatcgc cggctggacg aacgcctgaa
      421 ggccacagaa gaggcggtgt cgttgagcga aaccgtcgcg gccagcgccc tgctgcccta
      481 cgaagaggtc cgtgacttct ttcactacaa gaacaactac atccatagtc tcgacgtcgc
      541 cgctgaggcc ctgtcggaga agcttggcct ggccagcgac gtttcagccg agaccgcgct
      601 ggagcgccat ctaggcgcca gtcttgggat cacggtggcc cggtcaatgg aacctggcct
      661 cttgcggcgt tttgaccggg tgagcggaac cctgaccctt ggcgcggcgg acgcggcgac
      721 aacccgcacc ttccagatgg cgtatcagat tgtctccgaa gggctgtcag acgcggtgga
      781 gtccgagctt cgcgcagcgg ggtttcgcag tgagagcgcc gccaaggtgt gtcgtacggc
      841 gctgttcaac tacgccgccg gcgcgctcct gctgccctat gagcggttcc gcgcggcggc
      901 gcgggcggcg cgacatgaca tcgagcattt gagcctgacg ttccagacca gtctggaaca
      961 ggtgtgtcac cgcctcagca ccttgcagcg cccgggcgcc cggggggtgc cgt
//
LOCUS       DAOMEO010001319         1010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15105_length_1010_cov_1.921466, whole genome shotgun sequence.
ACCESSION   DAOMEO010001319 DAOMEO010000000
VERSION     DAOMEO010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15105_length_1010_cov_1.921466"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLH31_20760"
     CDS             complement(<1..733)
                     /locus_tag="PLH31_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR91658.1"
                     /translation="MCNRLSAPAFACLIAILAAIGVFGGNHAHAAPLASDRIAVTVSG
                     QGPDVVLIPGLASSGAVWDDTVKQLSGRYRVHVVQVAGFAGAPVAGNAEGPVLEPLVE
                     AVDGYIKSARLKSPAVIGHLMGGLTGLMLARRHPEALGRLMIVDSLPFYAVLFSPAAT
                     VETVRPQAVAMRDSMTTMSPETFAAQQAMTMPRLVKSPEGLKSALAWSKASSQSVVAR
                     AFHDVLTTDMRGELAAVKTPTTMLYP"
     gene            complement(794..988)
                     /locus_tag="PLH31_20765"
     CDS             complement(794..988)
                     /locus_tag="PLH31_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR91659.1"
                     /translation="MKNRLKVLRAERDWSQADLAERLEVSRQTINALETGKYDPSLPL
                     AFKIARLFSQPIETIFQDEA"
BASE COUNT          169 a          319 c          369 g          153 t
ORIGIN      
        1 aggggtagag catggtggtc ggggtcttta ccgccgctag ctcgccgcgc atgtcggtgg
       61 tcagcacgtc atgaaaggcg cgggccacga ccgattgcga cgaggccttc gaccacgcca
      121 gggcggattt caggccctcc ggcgacttga ccaggcgggg catggtcatg gcctgctggg
      181 cggcgaaggt ttcgggcgac atggtggtca tgctgtcgcg catggcgacg gcctggggcc
      241 tgacggtctc gaccgtggcg gcgggcgaga acagcactgc atagaacggc aggctgtcga
      301 cgatcatcag gcggcccagc gcctcggggt gacgacgggc cagcatcaga ccggtcaggc
      361 cgcccatcaa gtggccgatc accgccggag acttcagcct ggccgacttg atatagccgt
      421 cgaccgcctc gaccagcggc tccagcacgg gcccctcggc attgccggcg accggcgcgc
      481 cggcgaaacc ggcgacctgg acgacgtgga cgcgatagcg gcccgacagc tgcttgacgg
      541 tgtcgtccca caccgcgccg gacgaggcca ggccggggat cagcaccaca tcggggccct
      601 gcccgctcac cgttaccgca atgcggtcgg aggccagcgg ggccgcgtgg gcgtggtttc
      661 cgccaaagac accgatggcg gcgaggatgg cgatcaggca cgcgaaagcg ggcgccgaaa
      721 ggcggttgca catggcgaca atccttgttc gagggggacg gcggcggggt atccccgccg
      781 cgttcaggag aggctacgcc tcgtcctgga agatggtttc gatgggttgg ctgaagagac
      841 gggcgatctt gaaggccagc ggcaggctgg gatcatattt gcccgtctcc agggcgttga
      901 tggtctgacg cgagacctcc agccgctcgg ccagatcggc ctggctccag tcgcgctcgg
      961 cgcgcagcac cttgaggcga tttttcaccg gtacctccag cgcacgaggg
//
LOCUS       DAOMEO010001320         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15123_length_1009_cov_2.835430, whole genome shotgun sequence.
ACCESSION   DAOMEO010001320 DAOMEO010000000
VERSION     DAOMEO010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15123_length_1009_cov_2.835430"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="bamD"
                     /locus_tag="PLH31_20770"
     CDS             <1..374
                     /gene="bamD"
                     /locus_tag="PLH31_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQR91660.1"
                     /translation="YYLRQGQTLAGIGRFKTVIDRHQTTSHTPEALFRLVESYLTLGL
                     ADEAKRNGAVLGYNFPGDRWYSDAYRLLNDAGLKPAVEPLTPGAKKNLLDRLVRDKNA
                     TLAPPGEKPTKKDGMRGLLGL"
     gene            467..>1009
                     /locus_tag="PLH31_20775"
     CDS             467..>1009
                     /locus_tag="PLH31_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR91661.1"
                     /translation="MLIGLSIRDVVLIESLDLAIGPGLTALTGETGAGKSIILDALGL
                     ATGARADAGLVRRGAAGQASATAIFALPADHPAFAYLDDKGLDYGRDEDLVLRRQLSP
                     DGRSRAFVNDQATSVGVLKDLGALLLEVHGQHETVGLLDPRTHRTLLDAFGAVSVSAV
                     ASAWSGWRAAREKAEALRALA"
BASE COUNT          157 a          373 c          320 g          159 t
ORIGIN      
        1 gctattacct gcgtcagggc cagaccctgg ccgggatcgg ccgcttcaag accgtgatcg
       61 accgtcacca gaccacctcg catacgcccg aagccctgtt ccgtctggtc gaatcctatc
      121 tgaccctggg cctggccgat gaggccaagc gcaacggcgc ggtgctgggc tacaacttcc
      181 cgggcgatcg ctggtactct gacgcctaca ggttgctgaa tgacgccggc ctcaagcccg
      241 ccgtcgagcc gctgaccccg ggcgccaaga agaacctgct ggatcgtctg gtgcgcgaca
      301 agaacgccac cctggcccct cccggtgaaa agccgaccaa gaaggacggc atgcggggcc
      361 ttctggggct ctagccccgg ctcttcgcgg ccttgtggaa cacatgtcga acatcgaagc
      421 gtctccgcgc tttgaaaaac gctgattctg ggcgaatctc cgcgacatgc tgattggtct
      481 ctcaattcgc gacgtcgtgc tcatcgagag cctggacctg gccatcggtc cgggcctgac
      541 cgccctgacc ggtgaaaccg gcgcgggcaa gtcgatcatc ctcgacgccc tgggcctggc
      601 gaccggggca cgggccgacg ccggcctcgt tcgacgcggc gcggcgggcc aggcctcggc
      661 caccgccatc ttcgccctgc cggccgacca ccccgccttc gcctatctgg acgacaaggg
      721 cctggactat ggccgcgacg aggacctggt cctgcgccgc cagctgtcgc ccgacggccg
      781 ctccagagcc ttcgtcaacg accaggccac cagcgtcggc gtgctgaaag acctgggcgc
      841 cctgctgctg gaggtgcacg gccagcacga gactgtcggc ctgttggatc cccgcaccca
      901 ccgcaccctg ctggacgcgt tcggcgccgt gtccgtctcg gccgtcgcct cggcctggag
      961 cggctggcgc gccgcgcgcg aaaaggccga ggccctgcgc gccctggcc
//
LOCUS       DAOMEO010001321         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15129_length_1009_cov_2.174004, whole genome shotgun sequence.
ACCESSION   DAOMEO010001321 DAOMEO010000000
VERSION     DAOMEO010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15129_length_1009_cov_2.174004"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PLH31_20780"
     CDS             complement(<1..116)
                     /locus_tag="PLH31_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HQR91662.1"
                     /translation="MSLRDFGVLVLICLVWAGNNIISKIVVSHWGVPPLAYAA"
     gene            282..>1009
                     /locus_tag="PLH31_20785"
     CDS             282..>1009
                     /locus_tag="PLH31_20785"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR91663.1"
                     /translation="MATEHFDVLIVGAGLSGIGAGYHLQTHCPGKTYAILEGRAAIGG
                     TWDLFRYPGIRSDSDMYTLGYSFKPWKAAKAIADGPSILNYVRETASEHGIDRHIRYG
                     HHVKRASWSSETATWTVEAEHVGHSVSVTCNFLYMCAGYYDYAAGYTPDFAGVESFGG
                     QIVHPQHWPKGLDYAGKRVVVIGSGATAVTLVPEMAKTASHVTMLQRSPTYVVSRPAE
                     DGVANWLRSKLPAMTAYGITRWKNV"
BASE COUNT          199 a          343 c          311 g          156 t
ORIGIN      
        1 gcagcatagg ccagcggcgg cacgccccag tgggacacca cgatcttgga gatgatgttg
       61 ttgccggccc agaccagaca gatcaggacc agcacaccga aatcgcgtag ggacatgccc
      121 ccgcaatacc cgtcgaagac gaccgtgcgc aatgaaaacc gacacccggc cgtcgcgtcg
      181 cagttgacgc taggttgaaa cggtcgttcg ctggtgaaca ctgatcagat accaagacgt
      241 ccgggacagc cggaccgatc catcaggggg cggaaaacgg catggcgacc gagcatttcg
      301 acgttttgat cgtgggcgcg ggcctgtcgg gcatcggggc gggctatcac ctgcagacgc
      361 actgcccggg caagacctac gccatcctcg aaggccgcgc ggcgatcggc ggcacctggg
      421 acctgttccg ctatcccggc atccgctccg acagcgacat gtacaccctg ggctattcat
      481 tcaagccgtg gaaggccgcc aaggccatcg ccgatggccc ctcgatcctg aactatgtgc
      541 gcgagaccgc cagcgagcat ggcatcgacc gccacatccg ctacggccac cacgtcaagc
      601 gggccagctg gtcgtcggaa accgccacct ggacggtcga ggccgagcat gtcggccaca
      661 gcgtcagcgt gacctgcaac ttcctctata tgtgcgccgg ctattacgac tacgccgccg
      721 gctatacgcc cgactttgcc ggcgtggaga gcttcggcgg ccagatcgtg catccccagc
      781 attggcccaa gggcctcgac tatgctggca agcgcgtggt ggtgatcggc agcggcgcca
      841 ccgccgtcac cctggtgccg gaaatggcca agaccgccag ccacgtcacc atgctgcagc
      901 gttcgcccac ctatgtggtc tcccgtccgg ccgaggatgg cgtcgccaac tggctgcgca
      961 gcaagctgcc ggccatgacc gcctatggca tcacgcgctg gaagaacgt
//
LOCUS       DAOMEO010001322         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15133_length_1009_cov_1.901468, whole genome shotgun sequence.
ACCESSION   DAOMEO010001322 DAOMEO010000000
VERSION     DAOMEO010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_1009_cov_1.901468"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLH31_20790"
     CDS             <1..404
                     /locus_tag="PLH31_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR91664.1"
                     /translation="LGDALDARPAQLSGGMAMRVSLARALVTRPRLLLLDEPFAALDE
                     ITRRALADDVLVLASELSPALIFVTHNVEEAVYMAGRVVVMTPGPGRIAGEVAIKGPL
                     IRPAGFRTTEAFRQAVEAVSDLLAQAGARAA"
     gene            407..>1009
                     /locus_tag="PLH31_20795"
     CDS             407..>1009
                     /locus_tag="PLH31_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR91665.1"
                     /translation="MLSAFLSPLVLIGLLLAGWEIACARLAVPAYLLPPPSAIGTALI
                     EGWPLLLASAWGTLSTALLALVIASLLACALALAVSLSTLLEDAVRPIAVTLQVTPLV
                     AIAPLVTIWAGIDHPERAVIGLAAVVAFFPIFSGALAGLKAVDPDLARLFDLYGATPA
                     QRLWRLRLPSAVPALLEGHKVASGLAVIGAVVAEFVAGSGG"
BASE COUNT          108 a          377 c          334 g          190 t
ORIGIN      
        1 gcctgggcga cgccctcgac gcgcgccccg cccagttgtc gggcggtatg gccatgcgcg
       61 tctcgctggc ccgcgccctg gtcacccgcc cccgcctgct gctgctggac gagcccttcg
      121 cggcgctgga cgagatcacc cgccgcgccc tggccgatga tgtgctggtc ctggcgtcgg
      181 agctttcgcc ggccctgatc ttcgtgaccc ataatgtcga ggaagcggtc tacatggccg
      241 gccgcgttgt ggtcatgacc cctggccctg gccgcatcgc cggagaggtg gccatcaagg
      301 gccctctgat ccgccccgcc ggattccgga caactgaagc gttccgacag gctgtcgaag
      361 cggtgtctga cctgctggcc caggccggag cccgcgccgc gtgaggttgc tttcggcctt
      421 cctctcgccg ctcgtactga tcggtctgtt gctggcgggc tgggagatcg cgtgcgcccg
      481 ccttgccgtt ccggcctacc tgctgccccc gcccagcgcg atcggaacag ccctgatcga
      541 aggctggccg ctgcttctgg cctcggcctg gggcacgctg tcgacggcct tgctggccct
      601 ggtcatcgcc agccttctgg cctgcgccct ggccctggcc gtcagcctga gcaccctgct
      661 ggaagacgcc gttcgcccca tcgccgtcac cctgcaggtc acaccgctgg tcgccattgc
      721 gccgctggtg acgatctggg ccgggatcga ccaccccgag cgcgccgtga ttggactagc
      781 cgccgtagtc gccttctttc cgatcttctc cggcgccctg gccggcctga aggccgtgga
      841 cccggatctg gcgcgcctgt tcgaccttta tggcgccaca ccggcccagc gcctgtggcg
      901 gctgcgcctg ccctctgcgg tccccgccct gctggaaggg cacaaggtcg catcaggcct
      961 ggctgtcatt ggagccgtgg tggctgagtt cgtggccgga tcgggcggc
//
LOCUS       DAOMEO010001323         1009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15138_length_1009_cov_1.736897, whole genome shotgun sequence.
ACCESSION   DAOMEO010001323 DAOMEO010000000
VERSION     DAOMEO010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15138_length_1009_cov_1.736897"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PLH31_20800"
     CDS             <1..157
                     /locus_tag="PLH31_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR91666.1"
                     /translation="AALHLWLINRGVLIAPFHNMMLVSPVTEDASIDRLVAAVDGFAG
                     ALEGLSA"
     gene            166..>457
                     /locus_tag="PLH31_20805"
     CDS             166..>457
                     /locus_tag="PLH31_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQR91667.1"
                     /translation="MVADPQECRDFLAANPQVRFVDVFFTSMTGVPRGKRLRVHELQA
                     SYDYGRFLPGSVLVVDTTGADCEDTGLVWEDGDADRRARPVPGTLTLAPWLGD"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <468..>1009
                     /locus_tag="PLH31_20810"
     CDS             <468..>1009
                     /locus_tag="PLH31_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HQR91668.1"
                     /translation="LDGTPNDLDPRHVLQRVLDRYAADGLTPVAACELEYYLVDLERT
                     ADGDLQPPRSALTGQRPHGIEVYGLPELAAIAPFLRELWDTADVIGVPLEGAISEFAP
                     SQVELTLKHKPDALRACDDALLYKRMAKGVALRHGCEATFMAKPWADRAGNGFHVHVS
                     FNDDAGNNLCADEDPEGSPM"
BASE COUNT          149 a          346 c          347 g          157 t
ORIGIN      
        1 cgccgcgctt catctatggc tgatcaatcg gggcgtgctg atcgcgccct tccacaacat
       61 gatgctggtg tcgccggtga ctgaagacgc gtcgatcgac cgactggtcg cggcggtcga
      121 cgggttcgcc ggggcgctcg agggtctatc cgcatgacgt cccccatggt cgccgatccc
      181 caggaatgcc gcgacttcct cgccgccaat ccgcaggtgc ggttcgtcga cgtgttcttc
      241 accagcatga ccggcgtgcc gcgcggcaag cgcctgcggg tgcatgagct gcaggcgagc
      301 tatgactatg gccgcttcct gcctggctcg gtgctggtgg tcgacaccac cggggccgat
      361 tgcgaggaca cagggctggt ctgggaagac ggcgacgccg accgccgcgc gcggcccgtg
      421 cccggcacgc tgacgctggc gccctggctg ggcgacgnnn nnnnnnngct ggacgggacg
      481 cccaacgacc tggacccgcg ccatgtgctg caacgggtgc tggaccgcta cgcagccgat
      541 ggcctgacgc cggtggcggc ctgtgagctg gaatactacc tggtcgatct ggagcgcacc
      601 gccgatggcg atctgcagcc gccccgctcg gccctgaccg gtcagcgtcc gcacggcatc
      661 gaggtctatg gcctgcccga gctggcggcc atcgcgccgt tcctgcgcga gctgtgggac
      721 accgccgatg tgatcggcgt gccgctggaa ggcgcgatct cggagttcgc gcccagccag
      781 gtggagctga ccctcaagca caagccggac gccctgcggg cctgcgacga cgccctgctg
      841 tacaagcgca tggccaaggg cgtggccctg cgacacggct gcgaggccac cttcatggcc
      901 aagccgtggg cggatcgcgc gggcaacggc ttccacgtcc atgtcagctt caacgacgat
      961 gctggaaaca acctctgcgc cgacgaggac ccggagggct cgcccatgc
//
LOCUS       DAOMEO010001324         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15153_length_1008_cov_2.462749, whole genome shotgun sequence.
ACCESSION   DAOMEO010001324 DAOMEO010000000
VERSION     DAOMEO010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15153_length_1008_cov_2.462749"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLH31_20815"
     CDS             complement(<1..227)
                     /locus_tag="PLH31_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HQR91669.1"
                     /translation="MTISKIPTIRFYEEIGLMPTALRAANDRRIYGQEGVQRLAFIRH
                     ARQLGFSVEAIRNLLDLSDHPERHCGEANRLA"
     gene            278..>1008
                     /locus_tag="PLH31_20820"
     CDS             278..>1008
                     /locus_tag="PLH31_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HQR91670.1"
                     /translation="MHGEVMSQSCSPTPAVSSCCGADVKFDGATPAYRRALRGVIAIN
                     LVGFVVVLIGGLLQGSVSLSANALDFLADSATYAISLWAIGRSVAIRSGAALFKGVSL
                     GAVALSVAGYAVWRAWTGAPPSGQAISALGIFGALANLLAAALLVRFRDGDANVRSVW
                     LCTRNDLIQCLAVVATGALVWLTNSRWPDLIVGLLLAGVFLSSAYQIVRQARAERRAA
                     TSEPAPRPSASAWRGLVRPTVLTRRS"
BASE COUNT          135 a          331 c          337 g          205 t
ORIGIN      
        1 gccagtcggt tggcttcgcc gcaatggcgt tcagggtggt cggacagatc cagaaggttg
       61 cggatcgcct cgaccgagaa ccccaactgc cgggcgtgac ggatgaaagc caaccgctgg
      121 acgccctcct ggccatagat ccgccggtcg ttggcggcgc ggagcgcggt cggcatcagg
      181 ccgatctctt catagaatcg gatggttggg atcttggaaa tggtcatggc gtctcctgcg
      241 tcccaaaata agcgcttgct cctctagtgg ctagaggatg cacggtgagg tcatgagcca
      301 gtcctgttcc cccacccccg ccgtttcgag ctgctgcggc gccgacgtca aattcgacgg
      361 cgcgacgcca gcctaccgtc gcgccctgcg cggcgtgatc gccatcaacc ttgtcgggtt
      421 cgtcgtggtc ctgatcggcg ggctactaca agggtcggtg tcgctgtcgg ccaacgcgct
      481 cgacttcctc gccgatagcg ccacctacgc catcagcctt tgggcgatcg gtcgttcggt
      541 ggcgatccga agcggcgcgg ccctctttaa gggcgtcagc ctgggcgccg tggcgctgtc
      601 ggtcgcgggg tatgcggtct ggcgggcctg gacgggagcg ccaccgtccg gtcaggcgat
      661 ctcggccttg ggcattttcg gggccttggc caatctcctg gcggcggcct tgctcgttcg
      721 cttccgggac ggcgacgcca atgtccggtc ggtctggctg tgtacgcgca acgacctgat
      781 ccagtgtctc gcggtcgtcg ccaccggcgc tctggtttgg ctgaccaatt cacgttggcc
      841 cgacttgatc gtcggcttgc tcctggctgg tgtcttcctc agttcggcct accagatcgt
      901 tcgccaggcg cgcgccgaac gtcgcgccgc gacgagcgag cccgcgccgc gaccaagcgc
      961 ttcggcgtgg aggggcctgg ttcgcccgac agtcttgacc cgtcgttc
//
LOCUS       DAOMEO010001325         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15154_length_1008_cov_2.394544, whole genome shotgun sequence.
ACCESSION   DAOMEO010001325 DAOMEO010000000
VERSION     DAOMEO010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15154_length_1008_cov_2.394544"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLH31_20825"
     CDS             <1..389
                     /locus_tag="PLH31_20825"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQR91671.1"
                     /translation="DIEWADLGGNSTVIVGQAGIASGSIDYLGTIRARVGYAFDRVLP
                     YITGGVAYGKTDFGTIYGTSTSETNWGWVIGAGVEYAFTNNITAKLEYLYIDLDGSTY
                     TIPSTLGSIQADTNTSALKVGVNYKF"
     assembly_gap    489..498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            674..>1008
                     /locus_tag="PLH31_20830"
     CDS             674..>1008
                     /locus_tag="PLH31_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91672.1"
                     /translation="MKSYLLAGAALGILVSGPALAADLGRPVPAPVKAVAAAPPVFSW
                     TGFYVGGNAGYSWGSGQGAVGGVDPDGWLAGGQIGYNYQFQNNVLVGLEADIQGGDIS
                     GSTAGLDSKL"
BASE COUNT          155 a          336 c          316 g          191 t
ORIGIN      
        1 ccgacatcga gtgggccgac ctcggcggca acagcaccgt gatcgttggc caggctggca
       61 tcgcttccgg ctcgatcgac tatctcggca cgatccgcgc tcgcgttggc tatgccttcg
      121 accgcgtgct gccctacatc accggcggtg tggcttacgg caagaccgat ttcggcacca
      181 tctatggcac cagcacctcc gagaccaact ggggctgggt gatcggcgcg ggcgtcgagt
      241 acgccttcac caacaacatc actgccaagc tcgagtacct gtacatcgat ctcgacggct
      301 ccacctacac catcccgtcc accctgggtt ccatccaggc cgacaccaac acctcggccc
      361 tcaaggtcgg cgtgaactac aagttctgat cctcggtttc tccgagatcg cgaaccccgg
      421 gcgaaagccc ggggtttttt tatgtctgcg cgttgctgca gtccggagga ctcggcggcg
      481 ttttcggcnn nnnnnnnngt attgggcccg cttcgctccc caacccctgt ctctcatgga
      541 acggaccccg cgccgcgggg ttgaggggtc catggagaca ccttgccgtc gccaccgctt
      601 tgccacgtcc gggtcatcgg accgccgggg tggtgcggca acgttctcgc tgttcaacag
      661 tgaggtgcgt gcaatgaagt cgtatcttct ggccggggcg gcgctgggga ttctcgtttc
      721 cggaccggcc ctggcggccg atctcggacg gcctgtcccg gcgcccgtga aggcggtcgc
      781 ggcagcaccc cccgtctttt cctggaccgg attctatgtg ggcggcaatg ccggctacag
      841 ctggggctcg gggcagggcg ccgttggcgg cgtcgatccg gacggctggc tggccggcgg
      901 ccagatcggc tacaattacc agttccagaa caacgtgctg gtgggccttg aggccgatat
      961 ccagggcggc gacatcagcg gcagcaccgc cgggctcgac agcaagct
//
LOCUS       DAOMEO010001326         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15155_length_1008_cov_2.365163, whole genome shotgun sequence.
ACCESSION   DAOMEO010001326 DAOMEO010000000
VERSION     DAOMEO010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15155_length_1008_cov_2.365163"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..423)
                     /locus_tag="PLH31_20835"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="PLH31_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
     gene            complement(482..772)
                     /gene="groES"
                     /locus_tag="PLH31_20840"
     CDS             complement(482..772)
                     /gene="groES"
                     /locus_tag="PLH31_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002516095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQR91673.1"
                     /translation="MKFRPLGDRVLVKRVEEETKTKGGIIIPDTAKEKPQEGEVVAVG
                     PGARNDKGDVVALDVKAGDRILFGKWSGTEVKVDGQDLLIMKESDVLGVVEA"
BASE COUNT          162 a          293 c          323 g          230 t
ORIGIN      
        1 tgtccatggc cttggcgatc atgtcgccaa cttccttgtc gccgttggcc gagatggtgc
       61 caacctgagc gatttcggtg ttggtggtga ccttcttcga cgacttcttg atctcgtcga
      121 cgacgatgtg cacggccttg tcgatgccgc gcttcaggtc catcgggttc atgccggcgg
      181 cgaccgactt gaggccttcc tgaacgatgg cctgggccag gacggtggcg gtggtggtgc
      241 catcgccggc cttgtcgttg gtcttgctgg cgacttcgcg gatcatctgc cgaaggactt
      301 ttcgatcacg acgttgcggc ccttggggcc gagggtcacc ttcactgcat tggcgaggat
      361 gttgacgccg cgcagcatct tgtcgcgcgc gtccgaggag aaataaacgt ctttagcggc
      421 catgttggag cccttctaaa agagaaactt gaggtggaaa gtcgttcgga cggaggaggg
      481 cttaggcctc gaccacgccc agaacgtcgc tttccttcat gatcagcagg tcttgaccat
      541 cgaccttcac ttccgtgccc gaccacttgc cgaacaggat gcgatcgccg gccttcacgt
      601 ccagagcgac gacgtcgccc ttgtcgttgc gggcgccggg gccgacagcg acgacttcgc
      661 cttcctgcgg cttttccttg gcggtgtcgg ggatgatgat cccgcccttg gtcttggttt
      721 cttcttcgac gcgcttcacg aggacgcggt cgccaagggg acgaaatttc atggttgttc
      781 tccgtcggga cggttatcga taaaggggtc gttgacgcgg gaaacgctgc tgttagcagt
      841 cgcagccctt gagtgccaac gcgaggctga agtaggtccg aggccctccg gagtcaagga
      901 ctcgggagcg taatagcgag gtcgccgaca tgctggatca catcacgctg ggcgtggccg
      961 acttcgaccg gtcggtcgcc ttttacgaca aggccctggc cccgctgg
//
LOCUS       DAOMEO010001327         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15184_length_1007_cov_2.704832, whole genome shotgun sequence.
ACCESSION   DAOMEO010001327 DAOMEO010000000
VERSION     DAOMEO010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15184_length_1007_cov_2.704832"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1007)
                     /locus_tag="PLH31_20845"
     CDS             complement(<1..>1007)
                     /locus_tag="PLH31_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQR91674.1"
                     /translation="DIYPDEPRVDTKGSYVIENKTAGPLKEIHVRFDRDLKVRGLSIQ
                     GAWAKKTYDRFNYRIFAFDTPMVPGERRKMSFITERSQKGFPNSGAETRVVANGTFIN
                     NLEIAPILGMARDGLLVDRAKRRKYGLPAERRMARLGDVASMQFNGLRKDADFVSSDI
                     TVTTDADQTPIAPGYKVSEEVWSLRDDIKGPVKGEGDRRTVRFVTEAPILPFLSIQSA
                     RYAVSRETYKGIDLAIYYDPQHAWNIDRMKASMKGSLDYMQAEFSPYQFRQMRFLEFP
                     DYAQFAQAYAGTMPWSEGLFFISDYRDPEKIDMITYVGAHEIGHQWWAHQVIGADQQG
                     GS"
BASE COUNT          165 a          318 c          329 g          195 t
ORIGIN      
        1 gagccgccct gctggtcggc gccgatcacc tggtgggccc accactggtg gccgatctcg
       61 tgggcgccga cataggtgat catgtcgatc ttctcggggt cgcgataatc ggagatgaag
      121 aacagcccct ccgaccacgg catggtgccg gcataggcct gggcgaactg ggcgtagtcc
      181 ggaaactcca ggaaccgcat ctggcggaac tgatacgggc taaattcggc ctgcatatag
      241 tccagcgagc ccttcatgct ggccttcatg cggtcgatgt tccaggcgtg ctgggggtcg
      301 taatagatcg ccagatcaat gcccttgtag gtctcgcgcg acaccgcata gcgggctgac
      361 tggatcgaca ggaacggcag gatcggcgcc tcggtgacga agcgcaccgt gcggcgatcg
      421 ccctcgccct tcaccggacc cttgatgtcg tcccgcagcg accagacctc ttcggagacc
      481 ttgtatcccg gcgcgatcgg cgtctggtcg gcgtcggtgg tgacggtgat gtccgacgac
      541 acgaaatcgg cgtccttgcg caggccgttg aactgcatcg aggccacatc gcccagcctg
      601 gccatgcggc gctcggccgg caggccatat ttgcggcgct tggcgcggtc gaccagcagg
      661 ccgtcgcggg ccatgcccag gatcggcgcg atctccagat tgttgatgaa ggtcccgttg
      721 gccaccacgc gggtttcggc gccgctgttg gggaagccct tctgcgagcg ctcggtgatg
      781 aagctcatct tgcgccgctc gcccgggacc atcggcgtgt cgaaggcgaa gatccggtag
      841 ttgaaccggt cataggtctt cttcgcccaa gccccctgga tcgagagccc cctgaccttc
      901 aggtcgcggt cgaaccggac atggatttcc ttcagcggcc cggccgtctt gttctcgatg
      961 acataggagc ccttggtgtc gacgcgcggc tcgtccggat agatgtc
//
LOCUS       DAOMEO010001328         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15186_length_1007_cov_2.434874, whole genome shotgun sequence.
ACCESSION   DAOMEO010001328 DAOMEO010000000
VERSION     DAOMEO010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15186_length_1007_cov_2.434874"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="PLH31_20850"
     CDS             <1..>1007
                     /locus_tag="PLH31_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQR91675.1"
                     /translation="KAYVVQSGDTMFAIARRFTVTAAALAKENDVEIDAPIRRGQRLV
                     LPEGFKDKGPTKTAIAAPPYSATTTTLAGSAPTARAVQTPAPTQVAPTQVAPTQLAST
                     RPASPVASSASVTTTTTSVTGSVVRVAGPRQIHTVKSGDTLTAIARRFDMSVKDLAAA
                     NDLDPDKPLKLGARIKGPATTARAYVVQSGDTLTAITKRFSVTLKALAAENNLKTTAS
                     VKKGQKIVLPDGYKDRGPIKTTTTRPAAPSTTLAQGPAPARVEPQVTTPSAPIPYRPS
                     PSTPRASGPVAAMPVSPPPATSVRPIIETSAAPTEAEIIASGKGRFSWPLRGDIISTF
                     GV"
BASE COUNT          177 a          398 c          312 g          120 t
ORIGIN      
        1 aaggcctatg tggttcagtc cggcgacacg atgttcgcca tcgcgcgccg ctttaccgtc
       61 acggccgccg ccctggccaa ggaaaatgac gtcgagatcg acgcgccgat ccggcgtggc
      121 cagaggctgg tgctgcccga gggcttcaag gacaagggcc cgaccaagac cgccatcgcc
      181 gccccgccct acagcgcgac gacgaccaca ctggccggct cggcgcccac cgcgcgcgcc
      241 gtccagacgc cggccccgac ccaggtggcc ccgacccagg tggccccgac ccagctggca
      301 tcgacgcggc ccgccagccc ggtggccagt tcagcaagtg tcacgacgac gacgaccagc
      361 gtcaccggtt cggtggtccg cgtggccgga ccgcgccaga tccatacggt aaagtcgggc
      421 gacaccctga cggcgatcgc ccggcgcttc gacatgagcg tcaaggacct ggccgccgcc
      481 aacgacctcg atcccgacaa gcccctgaag ctgggcgcca ggatcaaggg gccggcgacc
      541 acggccagag cctatgtcgt ccagagcggc gacaccctga ccgccatcac caagcgcttc
      601 agcgtcaccc tcaaggcgct ggccgccgag aacaacctga agaccacggc ctctgtgaag
      661 aaggggcaga agatcgttct gcccgatggc tacaaggacc gcggtccgat caagaccacg
      721 acgacccggc cagccgcccc gtcaacgacc ctggcccagg ggcccgctcc ggcccgcgtc
      781 gagcctcagg tcacaacgcc ctcggcgccg atcccgtatc gtcccagccc ctcgacgccg
      841 cgcgccagcg gtccggtcgc ggccatgccg gtcagtccgc cgccggccac ctcggtgcgt
      901 ccgatcatcg agaccagcgc cgcgccgacc gaggccgaga tcatcgcctc gggcaagggc
      961 aggttctcct ggcccctgcg cggcgacatc atctccacct tcggcgt
//
LOCUS       DAOMEO010001329         1007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15196_length_1007_cov_1.712185, whole genome shotgun sequence.
ACCESSION   DAOMEO010001329 DAOMEO010000000
VERSION     DAOMEO010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15196_length_1007_cov_1.712185"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>660
                     /locus_tag="PLH31_20855"
     CDS             107..>660
                     /locus_tag="PLH31_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91676.1"
                     /translation="MTHNIDRARLMLMLNDLRLPAIKQNWPDFAERADKEGWPAARFL
                     AALAEHEIAERDRRRLARHLAEAQLLPGKTLDSFDFDVVPMISKAHVMAICAGDSWID
                     KGANLILIGGPGGGKTHLASAVGLALVENGWRVLFTRTSDLVQRLQVARRELALEAAI
                     ARLDRFDLLILDDLAYVSKDQAETS"
     assembly_gap    661..670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <671..927
                     /locus_tag="PLH31_20860"
     CDS             <671..927
                     /locus_tag="PLH31_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR91677.1"
                     /translation="ERRSTLITANQPFGAWGKVFPDPAMTLAAVDRLVHHATIFEINV
                     DSYRRRAALERKKKGAGRPQSYATINDLEAVSLPDNQNET"
BASE COUNT          199 a          339 c          280 g          179 t
ORIGIN      
        1 gcacccgctt cgcgccagat cccgcctcgc tgcccgaggt cgttgtccat ctcatgccgc
       61 tgatcgccta tgaggccctg cttggcaccg acatcggaga agcagcatga cccacaacat
      121 cgaccgggcc cgactcatgc tgatgctcaa cgatctccgc ctgcccgcaa tcaagcagaa
      181 ttggccggat tttgccgaac gggcggacaa ggagggctgg cctgccgcac gcttcctcgc
      241 ggccctcgcc gaacacgaga tcgccgaacg cgaccgccgc cgcttggcac gccatcttgc
      301 cgaggcccag ttgctgccgg gaaagaccct cgacagcttc gacttcgacg tggtgccgat
      361 gatctccaag gcgcacgtga tggcgatctg tgccggtgac agctggatcg acaaaggcgc
      421 caatctgatt cttatcggcg gacctggcgg cggcaagacg catctggcct ccgccgtcgg
      481 tctggcgctg gtggaaaacg gctggcgcgt gctgttcacc cgcacgtccg acctggtgca
      541 gagacttcag gtggcgcggc gcgaactggc attggaggcg gccatcgcca gactggatcg
      601 attcgatctg ctgatcctcg atgacctcgc ctatgtcagc aaggatcagg ctgagacctc
      661 nnnnnnnnnn acgaacgccg ctccaccctg atcaccgcca atcagccatt cggggcctgg
      721 ggcaaggtct tcccggatcc cgccatgacg ctggccgcag ttgatcgcct cgtccatcac
      781 gccacgatct tcgaaatcaa cgtcgacagc taccgccgtc gtgccgcact cgaacgcaag
      841 aaaaaaggcg ccggacggcc gcagagctac gcgacaatca acgatctcga agccgtgtcg
      901 ctgcccgaca atcaaaacga aacttagcgc ttgtcagcga caatcagacc gaacaacatc
      961 gttcccgttg cggtcacagt ttctcatcct gattgacgca ttttctc
//
LOCUS       DAOMEO010001330         1004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15265_length_1004_cov_1.789252, whole genome shotgun sequence.
ACCESSION   DAOMEO010001330 DAOMEO010000000
VERSION     DAOMEO010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15265_length_1004_cov_1.789252"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLH31_20865"
     CDS             <1..414
                     /locus_tag="PLH31_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR91678.1"
                     /translation="GHAGGSRLLSRGHSRPLKVRDCATLTDAVIATTDADACFDGAER
                     GAWLQVRAAAKLARLGCDAYAYAMVAMGTMDMVIEAGLKSWDIEAAIPLIQGAGGMVT
                     DWRGQPVGQNGGQIVISGDRRPLEEALVSLRRSAK"
     gene            complement(389..>1004)
                     /locus_tag="PLH31_20870"
     CDS             complement(389..>1004)
                     /locus_tag="PLH31_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR91679.1"
                     /translation="KPWIALGHSMGGCLTTLALAHGEPRFSACVLSAPMLGLNTGKRP
                     PRASRALAWLMARTGKAGDYTPGEQSDPFAHSFATDALTHDQARYERYRAQLLANRDL
                     ALGGATWGWVDFAFSACAWLRRSPGVEAISIPVTIVGAGLDSRVLNPDLRSIAQRIPK
                     GRYLELDGAFHEILIETDEHRARFWAAFDETVDAFAATSPTAAD"
BASE COUNT          168 a          342 c          351 g          143 t
ORIGIN      
        1 ggccatgccg gcggttcacg cctgctgtcg cgcggtcaca gccggcccct caaggtccgc
       61 gactgcgcga ccctgaccga cgccgtcatc gccaccaccg acgccgacgc ctgcttcgac
      121 ggggccgagc gcggcgcctg gctgcaggtg cgcgccgccg ccaagctggc ccgcctgggc
      181 tgcgacgcct atgcctacgc catggtcgcc atgggtacga tggacatggt catcgaagcc
      241 ggcctgaaat cctgggacat tgaagcggcc atccccctga tccagggcgc cggcggcatg
      301 gtcaccgact ggcgcggcca gccggtcggc cagaacggcg gacagatcgt gatctccgga
      361 gatcgcaggc cgctggaaga ggcgctggtt agtctgcggc ggtcggcgaa gtagccgcga
      421 aggcgtccac tgtctcgtca aaggccgccc agaaccgggc ccggtgctcg tcggtctcga
      481 tcagaatctc gtggaaggcg ccgtccagct ccagataacg ccccttggga atacgctggg
      541 cgatggatct gagatccggg ttcagcacac ggctgtccag ccctgcaccg acgatggtca
      601 ccgggatcga gattgcctca acgcccggtg agcggcgcag ccaggcgcag gcggagaagg
      661 caaaatccac ccagccccag gtcgcgccgc ccagagcaag gtcgcgattg gccagaagtt
      721 gcgcgcgata gcgctcgtag cgggcctgat cgtgggtcag ggcgtcggtg gcgaagctgt
      781 gggcgaaagg atcgctctgc tcgcccggtg tgtagtcgcc cgccttaccg gtgcgggcca
      841 tcagccaggc cagggcccgc gacgcgcgag gcggcctttt gcccgtattg aggcctagca
      901 tcggcgccga cagcacgcag gccgaaaagc ggggctcgcc atgggccagg gccagggtcg
      961 tcaggcaccc gcccatcgaa tgccccagag cgatccaggg cttg
//
LOCUS       DAOMEO010001331         1003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15278_length_1003_cov_2.665612, whole genome shotgun sequence.
ACCESSION   DAOMEO010001331 DAOMEO010000000
VERSION     DAOMEO010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15278_length_1003_cov_2.665612"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1003)
                     /locus_tag="PLH31_20875"
     CDS             complement(<1..>1003)
                     /locus_tag="PLH31_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR91680.1"
                     /translation="GLEVSVRPGEGFRPYSDDGRSLGVAAVQNVVGVLPGQDRELPAV
                     LVMSHYDSVHNSPGAADDALGAAAALEIARALKAGPTPARDVIFLFTDAEEAGLLGAD
                     AFFARDPTAQRVGVVLNMEARGDAGRAAMFQTGPENGALLALMAREAKGPSANSLAST
                     VYERMPNDTDFTHSVKAGLPGLNFALIDNQLAYHTPLSTPERLDQGSLQHMGDQVLPI
                     TRAVADGQSLPARSPNAIYSDVLGFFLISYSTAVGWGLLGLAAVMIGFVSWRALSGGA
                     TSPLDIVRGVAGAILLLLASALVLHLLGRLLNVGDGQKLYDLLGRFDLLLLGAGALGV
                     "
BASE COUNT          171 a          352 c          350 g          130 t
ORIGIN      
        1 gacgccgagc gcgccagcgc ccagcagcag aaggtcaaag cggcccagca gatcgtacag
       61 cttttgccca tcgccgacgt tcagcaggcg gcccagcagg tgcaggacca gagcgctggc
      121 cagcagcagc aggatggccc cggccacgcc cctcacgatg tcgagcggcg aggtcgcgcc
      181 gccgctcagt gcgcgccagc tcacgaagcc gatcatcacg gcggccagcc ccagcagccc
      241 ccagccgacg gccgtcgaat agctgatcag gaaaaagccc agcacatcgg aatagatcgc
      301 attggggctc ctggccggca ggctctggcc atcggccacc gcccgggtga tcggcagcac
      361 ctggtcgccc atgtgctgca gactgccctg gtccagccgc tccggcgtcg acagcggcgt
      421 gtgataggcc agctgattgt cgatcagggc aaagttcagc cccggcagtc cggccttcac
      481 cgaatgggtg aagtcggtgt cattgggcat gcgttcatag acggtcgagg ccagggaatt
      541 ggccgagggg cccttcgcct cgcgggccat cagggccagc agggcgccgt tctcgggacc
      601 ggtctggaac atggccgccc ggccggcgtc gccccgagcc tccatgttca gcaccacccc
      661 gacccgctgg gcggtaggat cgcgggcgaa gaaggcgtcg gcgcccagca ggcccgcctc
      721 ttcggcgtcg gtgaacagga agatcacgtc gcgggccggc gtcggacccg ccttcagggc
      781 gcgggcgatc tccagagcgg cggccgcgcc cagggcgtcg tcggcggcgc cgggggaatt
      841 gtggacggaa tcgtagtggc tcatcaccag cacggcgggt agctcgcggt cctgtcccgg
      901 caggacgcca accacgttct gcacggcggc cacgcccagc gagcggccgt cgtcggaata
      961 gggccggaag ccttcaccgg gccggaccga gacctcaagg ccc
//
LOCUS       DAOMEO010001332         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15329_length_1002_cov_1.193242, whole genome shotgun sequence.
ACCESSION   DAOMEO010001332 DAOMEO010000000
VERSION     DAOMEO010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15329_length_1002_cov_1.193242"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLH31_20880"
     CDS             complement(<1..715)
                     /locus_tag="PLH31_20880"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR91681.1"
                     /translation="MDLFAREFPMPRGQLRSLHIAERLSGRDICGRLGAPAQAVAQQL
                     LDALLLPIPKAAEEDPTPVEAPLNPSQDEAVRHEGPALLLEAGPGTGKTRTLVARVRH
                     LLDHNVDPENILVLTFSNRAAAELVDRISLVSPKAAATMWIGTFHSFGLDLVRRFSDR
                     LERPGEPRLLDTSEAVMLMEREFLQLDFEHYRDLNDPTRLIAELLRAVSRAKDEVVCP
                     IRYRELADDMPGGSQSIERA"
     gene            730..>1002
                     /locus_tag="PLH31_20885"
     CDS             730..>1002
                     /locus_tag="PLH31_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR91682.1"
                     /translation="MRDQERVFEVVLRRLVKDKPVRGRSPDGAPAPQPGVVFWDWYNS
                     DAFRLSQSPRHLEWPPWVGATGDGSIVELRGVSPGRFNRRQRRAPRC"
BASE COUNT          180 a          299 c          343 g          180 t
ORIGIN      
        1 tggcgcgctc gatcgactgg gagccgccag gcatgtcgtc ggccaattca cgatagcgga
       61 tagggcagac gacctcgtcc tttgcgcgtg agacggcgcg gagcagttcg gcgatcaggc
      121 gtgtgggatc gttcaggtcg cgataatgct caaagtcgag ctgaaggaac tcgcgttcca
      181 tcaacatgac ggcttcggac gtatcgagta ggcgcggctc gccgggacgc tcaagcctgt
      241 cgctgaagcg acgaacaagg tcaagcccga aggagtgaaa ggtgccgatc cacattgtgg
      301 cggcggcctt ggggctcacc aagctgatgc gatccacgag ctccgcggct gcgcggttcg
      361 agaacgtcag aacaaggatg ttttcgggat cgacgttgtg gtccaggaga tggcgcacgc
      421 gcgcgacgag cgtcctggtc tttcccgtgc cgggtccggc ttccagcaac aaagccggtc
      481 cctcatggcg aaccgcctcg tcctgggagg gattgagcgg cgcctcgaca ggcgtgggat
      541 cctcttcggc ggctttgggt atcggcagca gcagcgcgtc caacaattgc tgcgcgaccg
      601 cttgggcagg tgctccgaga cgtccgcaga tgtcgcggcc agacaaccgt tctgcaatat
      661 gaagcgagcg gagttggccg cgcggcatcg ggaactcgcg cgcgaacaga tccattgtga
      721 cttcgcgcca tgcgcgatca ggaacgcgtc ttcgaagtcg ttctgcgtcg cctcgtgaaa
      781 gataaaccgg tcaggggccg gagcccagac ggcgcgcccg ctccacagcc tggggtcgtc
      841 ttttgggact ggtacaacag tgatgccttc cgtttgagcc agagcccgcg ccatctcgag
      901 tggccgccat gggttggcgc caccggcgac ggttcgatcg tggagctgcg cggcgtgtcg
      961 ccgggccgct tcaatcgccg tcaacgacgg gctccaaggt gc
//
LOCUS       DAOMEO010001333         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15335_length_1001_cov_2.393235, whole genome shotgun sequence.
ACCESSION   DAOMEO010001333 DAOMEO010000000
VERSION     DAOMEO010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15335_length_1001_cov_2.393235"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLH31_20890"
     CDS             complement(<1..366)
                     /locus_tag="PLH31_20890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR91683.1"
                     /translation="MLKKISGNRAAIIGFALLYLAFCISYIDRAAMSLALTQVGKEFS
                     LKPDELGAVLSIFFLGYALMQIPGGWLADRFGSKYVVIVTIAAWSVFTILTSQAWSLV
                     SLLAIRFIFGLAEGGFPSAS"
     gene            complement(436..>1001)
                     /locus_tag="PLH31_20895"
     CDS             complement(436..>1001)
                     /locus_tag="PLH31_20895"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQR91684.1"
                     /translation="ADDAFAVLRASLFLSMFEATYRAQPHMLGANLKANVEEGLGYSL
                     SDYARAAAAQTRIYRSYQSFFDRYDVLVSPTITLSPRPWSEPYPLEIDGVATRSYFHW
                     LALAYAVTLAGHPAISLPLGVDEAGMPFGLQIVGPRGGDARLLGIARALESAFADDRR
                     LCRPSPDLPMLRAARPLREAADFMTWE"
BASE COUNT          210 a          315 c          335 g          141 t
ORIGIN      
        1 gctggctgag gggaagccgc cctccgccaa cccgaaaatg aaccggatgg ccagcagcga
       61 gaccagagac cacgcctggc tggtgaggat ggtgaacacc gaccaggcgg cgatggtgac
      121 gatcaccaca tatttcgagc cgaagcggtc cgccagccag ccaccgggga tctgcatcag
      181 cgcatagccg agaaagaaga tactaagcac ggcgcccagt tcatcgggct tcaggctgaa
      241 ctccttgccc acctgcgtca gcgccagcga catggcggcc cggtcgatat aggagatgca
      301 gaaggcgaga tagagcagcg cgaagccgat gatggcggcc ctgttgccgg aaattttctt
      361 caacatggaa ggcctcggaa gtttgttatt ggctgaacag cgcgtccgtt ggccggacgt
      421 cgcgtaaacg ggtgcctatt cccaagtcat gaaatccgcc gcttcccgca gcggccgcgc
      481 ggcgcgcagc atcggcagat cgggcgacgg gcgacagagc cgacgatcat cagcgaaggc
      541 ggattccagc gccctggcaa tgccgagcag ccgggcatcc ccaccgcgcg gccccacgat
      601 ctggaggccg aagggcatgc ccgcctcatc cacgcccagc ggcaggctga tggccgggtg
      661 gccggccagc gtcaccgcat aggccagcgc cagccaatgg aaatacgagc gggtcgcaac
      721 cccgtcgatc tccagcggat agggctccga ccacggacgc ggactgagcg tgatggtcgg
      781 gctgaccagg acgtcatagc ggtcgaagaa gctctgatag gatcggtaga tgcgggtctg
      841 ggcggcggcg gcgcgagcat aatcggagag gctgtagccc agcccctcct ccacattcgc
      901 cttcagattg gcccccagca tgtggggttg ggcgcgatag gtggcctcga acatggacag
      961 gaacagcgac gcccgcagca ccgcgaaggc gtcgtccgcg c
//
LOCUS       DAOMEO010001334         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15336_length_1001_cov_2.359408, whole genome shotgun sequence.
ACCESSION   DAOMEO010001334 DAOMEO010000000
VERSION     DAOMEO010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15336_length_1001_cov_2.359408"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLH31_20900"
     CDS             <1..629
                     /locus_tag="PLH31_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="12-oxophytodienoate reductase"
                     /protein_id="HQR91685.1"
                     /translation="KALGFDAIELHGAHGYLIDQFFWEGTNVRTDGFGGPTIGERSKF
                     AAEILKAVRVAVGPDYPVIIRLSQWKQQEYTARLATTPKEMEAWLQPLADAGADIFHC
                     SQRRFWEPEFEGSDLNFAGWAKKLTGAPTITVGSVGLSGEFIAAFGGEGSQPASIDGL
                     LERLDRGEFDLVGVGRALLQDPEWVVKIHEGRTNELKSFEREALGVLY"
BASE COUNT          158 a          331 c          332 g          180 t
ORIGIN      
        1 ccaaggccct gggcttcgac gccatcgaac tgcacggcgc gcacggctat ctgatcgacc
       61 agttcttctg ggagggcacc aacgtccgta ccgacggctt tggcggcccg accattggcg
      121 agcggagcaa gttcgccgcc gagatcctga aagccgtccg cgtcgccgtt ggccccgact
      181 atccggtgat catccgcctg tcgcagtgga agcagcagga atacactgcc cgtctggcga
      241 cgacgcctaa ggaaatggaa gcctggctgc agcccttggc cgacgccggc gccgacatct
      301 tccactgcag ccagcgccgc ttctgggagc cggagtttga aggcagcgac ctgaacttcg
      361 ccggctgggc caagaagctg accggcgccc cgaccatcac cgtgggctcg gtgggcctgt
      421 cgggcgagtt catcgcggcc ttcggcggtg agggcagcca gcccgcctcg atcgatggtc
      481 tgctggagcg cctggatcgc ggcgagttcg acctggtcgg cgtgggccgc gccctgctgc
      541 aggatccgga gtgggtcgtg aagatccacg agggtcgcac caacgagctg aagagcttcg
      601 aacgcgaagc cctgggcgtt ctttactagg gaccatcccc cctgttccgg ggctgtccgt
      661 caggacggtc ccggcccatg gttgcgttac gggttgagcc gcgctggagc cgctcacccc
      721 ggggaggcgc gtcatgtcct ggttgcccta tctcgccggc ctgttcgcga tgtcggccct
      781 gatcctgctg atcatcggcg cgctgctgaa ggactgaggg cgctccaagt cctccccctt
      841 cagggtaggg ctatcgcata tgagtttgcg cccagaaaag ttgtcatccc ggacaagcca
      901 ggcgaagccg gcgccgatcc gggaccgact tggaacacgg cgctcaggcg tcggtcccgg
      961 atcttcgcgc tgcgcgctcg tccgggatga caaccgtttt t
//
LOCUS       DAOMEO010001335         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15338_length_1001_cov_2.070825, whole genome shotgun sequence.
ACCESSION   DAOMEO010001335 DAOMEO010000000
VERSION     DAOMEO010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15338_length_1001_cov_2.070825"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLH31_20905"
     CDS             complement(<1..894)
                     /locus_tag="PLH31_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HQR91686.1"
                     /translation="MPRKTKTTAAADKPAQPQFSAELLEQLIPGPVTPAELEGIFQQF
                     KKSVLERALGAEMSHHLGYAPGQAKPEGAAANHRNGKSAKTVLTDVGALRIDVPRDRE
                     GSFEPQLIGKHERRLTGFDDKIIAMYARGMTVREIQGFLAEMYSVDVSPDLISSVTDA
                     VMSEVTAWQSRPLESMYPVVFFDALRVKIREDAVVRSKAVYLALGVLPDGSRDILGIW
                     IENTEGAKFWMKVFNDLKTRGVHDILIAVTDGLKGMPEALQAVFPATTLQTCIVHLIR
                     GSLDFASWKDRKGLAAAIKPIY"
BASE COUNT          169 a          318 c          308 g          206 t
ORIGIN      
        1 gtagatcggc ttgatggctg cggccaggcc cttgcggtcc ttccatgacg caaagtccag
       61 gctgccccgg atcagatgga cgatgcaggt ctgcagcgtc gtggccggga acaccgcttg
      121 cagtgcctcg ggcatgccct tgaggccatc ggtcacggcg atcaggatgt catggacccc
      181 gcgcgtcttc agatcgttga acaccttcat ccagaacttg gccccctcgg tgttctcgat
      241 ccagattccc aggatgtcgc ggctaccatc gggcagcacg cccagggcta gatagacggc
      301 cttggagcgc acgacagcat cttcgcggat tttgacccgc agcgcgtcaa agaacaccac
      361 cgggtacatc gactccagcg ggcggctctg ccaggccgtg acctcgctca tcaccgcgtc
      421 ggtgacactg ctgatgagat cgggcgagac atcgaccgag tacatctcgg caagaaaacc
      481 ctggatctcg cgcaccgtca tgccgcgtgc gtacatggca atgatcttgt cgtcaaagcc
      541 cgtcaagcgg cgctcatgct tgccaatgag ctgtggctcg aacgagccct cgcggtctcg
      601 gggaacgtcg atgcgcaagg cgccgacgtc ggtcagcacg gtcttggcgc tcttgccgtt
      661 gcggtgattg gccgcagccc cttcgggctt ggcctggccg ggcgcgtagc ccaggtggtg
      721 actcatctcg gcgcccagcg cgcgttcaag aaccgacttc ttgaactgct ggaagatgcc
      781 ctcaagctcg gctggcgtta ccggccctgg gatgagctgc tccagcagct cggctgaaaa
      841 ttgcggctgt gccggcttgt ccgccgctgc ggtggtcttc gtcttgcgtg gcattcatgc
      901 tccttgttga catgctatgc ctcacacaca aagtttctga caggctcgtc tggtgagtcg
      961 cttgcggccc tgatttgcgg gaaatcccgc cgcattgggc c
//
LOCUS       DAOMEO010001336         1001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Caulobacter sp. isolate AMR_MDS_5026
            NODE_15340_length_1001_cov_1.819239, whole genome shotgun sequence.
ACCESSION   DAOMEO010001336 DAOMEO010000000
VERSION     DAOMEO010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867185
            Sequence Read Archive: SRR5352525
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Caulobacter sp. (wastewater metagenome)
  ORGANISM  Caulobacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
            Caulobacteraceae; Caulobacter.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:05:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,156
            Genes (coding)                    :: 4,015
            CDSs (with protein)               :: 4,015
            Genes (RNA)                       :: 26
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 85 of 141
            Pseudo Genes (incomplete)         :: 51 of 141
            Pseudo Genes (internal stop)      :: 16 of 141
            Pseudo Genes (multiple problems)  :: 9 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Caulobacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15340_length_1001_cov_1.819239"
                     /isolate="AMR_MDS_5026"
                     /isolation_source="Mine wastewater"
                     /db_xref="taxon:78"
                     /environmental_sample
                     /geo_loc_name="Canada"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-11-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1001)
                     /locus_tag="PLH31_20910"
     CDS             complement(<1..>1001)
                     /locus_tag="PLH31_20910"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQR91687.1"
                     /translation="EKTEKKPSVTVVNVAAADPVKADKVYEGLNALADAILFSRDLVS
                     EPANILHPEEFAARAKSLESLGLEVEILGEAEMAKLGMGSLLGVGQGSVRESQLVIMK
                     WMGAADKSAQPIAFVGKGVTFDTGGISIKPADGMEDMKWDMGGAAAVTGVMHALAGRK
                     ARVNAIGVLGLVENMPDGNAQRPGDVVTSMSGQTIEVINTDAEGRLVLADALRYTQER
                     FKPKFMIDLATLTGAIIISLGHDYAGLFSNNDGLSEKLLAAGKAERENLWRMPLPAAY
                     EKQIESPIADMKNIGGRPAGSITAGLFLQKFVNGVPWAHLDIAAVAWRKPSTDPTVP"
BASE COUNT          150 a          328 c          334 g          189 t
ORIGIN      
        1 cggcacggtg ggatcggtcg aaggcttgcg ccaggcgacg gcggcgatgt ccaggtgcgc
       61 ccatggcacg ccattgacga acttttgcag gaacaggccg gcggtgatcg aaccggcggg
      121 gcggccgccg atgttcttca tgtcggcgat cgggctttcg atctgcttct cgtaagcagc
      181 cggcagcggc atccgccaca ggttctcgcg ctcggccttg cccgccgcga gaagtttctc
      241 ggacaggcca tcgttattgc tgaacaaacc ggcgtagtca tgacccagcg aaatgatgat
      301 cgcgccggtc agggtggcca ggtcgatcat gaacttcggc ttgaaccgtt cctgcgtgta
      361 ccggagggcg tcggccagga cgaggcgacc ctcggcgtcg gtattgatga cctcgatggt
      421 ctggcctgac atcgaggtga ccacgtcgcc gggacgctgg gcgttgccgt cgggcatgtt
      481 ctcgaccagg cccagcacgc cgatggcgtt gaccctggcc ttgcggccgg ccagggcgtg
      541 catgacgccg gtcacggcgg ccgcgccgcc catgtcccac ttcatatctt ccatgccgtc
      601 ggccggcttg atgctgatgc cgccggtgtc gaaggtgaca cccttgccga cgaaggcgat
      661 gggctgggcg gacttatcgg ccgcgcccat ccacttcatg atgaccagct ggctctcgcg
      721 cacgctgccc tgccccacgc ccagcaggct gcccatgccg agtttcgcca tctcggcctc
      781 gcccaggatc tcgacctcca gacccaggct ctccaggctc ttggcgcgag cggcgaactc
      841 ttcggggtgc aggatattgg ccggctcgga gaccaggtcg cggctgaaca ggatggcgtc
      901 ggccagggcg ttgagccctt catagacctt gtcggccttg accggatcgg cggcggcgac
      961 gttgaccacg gtgaccgacg gcttcttctc ggtcttctcg g
//
